LOCUS       DAMJWP010000001        86192 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.001, whole genome shotgun sequence.
ACCESSION   DAMJWP010000001 DAMJWP010000000
VERSION     DAMJWP010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 86192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 86192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86192
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.001"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            110..301
                     /locus_tag="PC700_000001"
     CDS             110..301
                     /locus_tag="PC700_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HDF2579336.1"
                     /translation="MKKVALILAIIGALNWGAIGILGTDLIGSIFGGTYEMVSRIIYF
                     IVGLAGLYLIPSLMSDDRE"
     gene            456..1865
                     /locus_tag="PC700_000002"
     CDS             456..1865
                     /locus_tag="PC700_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2579337.1"
                     /translation="MFIINELNKIVNEEVISFHMPGHKKGQVYKKLGYKDILKNLYKM
                     DTTEILGTDNLHFPEGIIKNSQKKASRVFKSDYTCYLVNGSSCGIQSAIMSVCSPKSK
                     IITNRDCHQSVINGCILGDIDIEYVPCEISEETNVLKGISISNVIDIIDKNLDAKAVL
                     LTYPTYYGMTYDLEYICNYAHSKKMVVIVDEAHGAHLGLSERLPKTALEQGADIVVQS
                     THKTLPAFTQSSMIHIQGDRVDIDKILNMLRITESSSPSYLLLSSLELAVDIYENKGK
                     ELMEELLNNIRIFKDKVDSNINIYDTNDKTKIFISSKNMGLTGYELENILRNKYNIQV
                     ELSNYYGVLLICTIGNTAEDFSSLETALNNVVKEDFKFEKLENKKYPVVIPKKILTPR
                     EAFYKTKKSVKIYDSIGKICGECIVPYPPGISVISPGEVISKEIIDYLMFCHSKGMIV
                     SGLKDVDLNFIQVIDLEDI"
     gene            1996..2685
                     /locus_tag="PC700_000003"
     CDS             1996..2685
                     /locus_tag="PC700_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HDF2579338.1"
                     /translation="MKGKLIIIESGSDASGKATQTEILYERLKKEGYMIKKVEYPNYE
                     SPSSALVKMYLKGDFGKNASDVDPYIASTFFTADRYASFKTDWEEFYNEGGIIIADRY
                     TTSNMVHQASKMDICDREEYLNWLADYEFNLFKIPQPDCVIFLDVPIEFSKKLMENRK
                     NKITGEEKKDIHESDIEYLEKSYNNALYIADKYDWKKINCVLDNKLRSIESIHNEIYK
                     IVLDSIKPMER"
     gene            2688..3623
                     /locus_tag="PC700_000004"
     CDS             2688..3623
                     /locus_tag="PC700_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDF2579339.1"
                     /translation="MYFENIIGQDFAKKYLTNSIKKNKLNNAYMFEGMDGIGKKKFAD
                     ELSKLLLDYENLENSPDYVLIKPDGNSIKIAQIRNLQSDIVIRPHKDYKIYIINNAEK
                     MTVEAQNALLKTLEEPPNYAIIILVTNNKESLLETIKSRCDIIKFSPIPIEDLKRYLI
                     NTGIEEERAQLLAIFSRGSIENALNLSQSSEFSMMREDIQQYIQIMLDKNIVEILNIP
                     NNMEKYRGKIIALLDMMINYFRDIILLKENVNKNMLINVDKLVFIQNMSGKISYSQLS
                     KIIDIIEDAKSKIKSNCNFNISIQVMSLNIYEVIK"
     gene            3620..4513
                     /locus_tag="PC700_000005"
     CDS             3620..4513
                     /locus_tag="PC700_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HDF2579340.1"
                     /translation="MIKIVGIRFKSAGKIYYFDPVDFNIEQDMDVVVETARGLEYGKV
                     VVGPKDMDESELVSPLKPIIRIATEEDKKVYLENKEKAKETFELCQQKIKEHELTMFL
                     IDCEYTFDRNKLIFYFTAEGRIDFRELVKDLAAIFKTRIELRQIGVRDEAKSIGGLGP
                     CGRKLCCSSWLGDFQPVSIKMAKDQSLSLNPTKISGICGRLFCCLKYEHDVYSEAIEK
                     MPVVGALVQSDDGKGKVVEVNPLLEQIKVEFQDKTVKIYTREDIKILREPKKCDGCGG
                     KCKDNDGLDEATLRELKKLED"
     gene            4591..5337
                     /locus_tag="PC700_000006"
     CDS             4591..5337
                     /locus_tag="PC700_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HDF2579341.1"
                     /translation="MQLKDSERIDDLQLKGLKIIQDTNGFCFGIDAVLLANFTKIKKD
                     AKVVDLGTGTGIIPILIAGKSEAKKIIGVEIQEDVYEMATRSIKLNGLEERVEIINED
                     IKYIDKVLDINGYHVVTSNPPYMHIDGIKNPNDKKAISRHEVRCNLEDVIRASSRLVM
                     PRGKFFMIHRPTRLVDIITLGRKYKLEPKVIQFVHPRPQKAPNLVLVQFVKDGRPELK
                     ILDPLYVYGEDGNYTKELKAIYNNKDIGEL"
     gene            5338..6171
                     /gene="rsmI"
                     /locus_tag="PC700_000007"
     CDS             5338..6171
                     /gene="rsmI"
                     /locus_tag="PC700_000007"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDF2579342.1"
                     /translation="MSGKLYICPTPIGNLEDITYRTLRTLKEVDLIAAEDTRHSIKLL
                     NHFEISKPLTSYHEHNKDSKGDYLINKLIDGENIALISDAGMPGISDPGEEIIKQAIQ
                     NNIEIEVLPGATAFVTALVGSGMDTHRFVFEGFLDRDKKVRKAQLEELKEESRTIIFY
                     ESPHRLKDTLKDMLKILGNRNISINRELTKKYQEIIRENIETTIKIFEEKEVRGEFVL
                     IVEGFHGEKSKKENYDGLTDREYVLKLIESGMNKKDAIKIVCKDRKLKKDVVYKQVLD
                     L"
     gene            6194..6898
                     /locus_tag="PC700_000008"
     CDS             6194..6898
                     /locus_tag="PC700_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HDF2579343.1"
                     /translation="MEIGKIIIESLKREDTHKIFIELDNKHELEKIIPKISCMKSVGE
                     CKYHVVNCFEHSINALKELEIVLNDKDFFPIHLRKHVSNYLNTYIEEGINKLHVLKLG
                     TFLHDIGKPDSMTLDETGRVHFTNHEKIGAQIIDNMGIKLELSTETYRLISKYVRYHM
                     ILLSLYKKNDLSRKELINVFNLVDEDTIGVIMLGYADIVSTVKLLGKSGEVSVLKTYM
                     EYILTNYLYKSNYTHV"
     gene            complement(7086..7514)
                     /locus_tag="PC700_000009"
     CDS             complement(7086..7514)
                     /locus_tag="PC700_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HDF2579344.1"
                     /translation="MDVTDYRIIEILQDDGRISMKDLGKIVGLTSPAVSERVKRLEES
                     GVIEGYKAIVNPDSLGRVIKAFIHISLPSNGYTEFIESAAKDPRIVECHHITGDDCLL
                     LKVIVKDMYELENVIDTIKKIGSTKTSVILSTPIQAKSIL"
     gene            complement(7551..8981)
                     /locus_tag="PC700_000010"
     CDS             complement(7551..8981)
                     /locus_tag="PC700_000010"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDF2579345.1"
                     /translation="MRNLTLLTDLYQLTMLNGYFEKNIHEDIVVFDMFFRKNACDGGY
                     TIVCGIDQVVEYIDNLHFSDEDLEYLKNLNLFSDKFLKFLKEFKFTGDIYAVEEGTIM
                     FPNEPLITVKAPLYQAQLIETALLTIVNFQSLIATKASRVCFAAQGDPVFEFGLRRAQ
                     GPDAGIYGARAAVVGGCAGTANVLAGKMFDIPIIGTQAHSWVQKFDNELEAFQAYADV
                     YPDKCLLLVDTYDVLNSGVPNAIKVFKNISEKGYKPMGIRLDSGDLAYLSKEAKKQLD
                     NAGFSDISITASNDLDEYTITSLKAEGATINSWGVGTKLITSFDSPSLGGVYKLAASC
                     EKGVLEPKIKISENPEKINNPGYKKVIRIYNEDNKAEADLIMLHDEVIDESKPLEIFH
                     PTYTWKTKVFTNYKVKELLKPLYIKGRCKYNKKAVLEIKNHVQYELSTIWEQYKRLSK
                     PHIYKVDLSRNLWYLKTQMIDSKKVL"
     gene            9113..9697
                     /locus_tag="PC700_000011"
     CDS             9113..9697
                     /locus_tag="PC700_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein A"
                     /protein_id="HDF2579346.1"
                     /translation="MIIIGKIWFYMVSIGIIGSLISKNMGELNKVILSETSKGIEFAI
                     SLAGIMALWMGIMNIAKESGLIEKIGQKLNPIMRRLFPSIPKGHKAMSYIVMNIALNM
                     LGAGNGATAFGLKAMSELQTLNNKKDTATNDMIMFMVINISSIQIIPFTIIKLRMDMG
                     AQNPSEIIFTTLFATIISTAVAIITCKFFQRRYR"
     gene            9713..10234
                     /locus_tag="PC700_000012"
     CDS             9713..10234
                     /locus_tag="PC700_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HDF2579347.1"
                     /translation="MEIFSNLLIPIIILYIVVYGKYKKIDVYDSFVKGAIDGLKAAWD
                     ILPYIIGIFLAIGIFKTGKGLDMLEWLFTPIANMMSIPKELIGLISVKPLSGSGALGM
                     YSELANRVGIGSLVEKMGATIVGSSETIFYTMAIYYGSLKIKNTRHTLSCAMISHVAG
                     VIAAVFICYVIFV"
     misc_binding    10300..10546
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10630..12567
                     /gene="metG"
                     /locus_tag="PC700_000013"
     CDS             10630..12567
                     /gene="metG"
                     /locus_tag="PC700_000013"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDF2579348.1"
                     /translation="MSKPSFYVTTPIYYPSGGLHIGHTYSTVAADTIARFKRFCGYDV
                     KFLTGTDEHGEKIQKKAIEQGMSEIEYLDGMIKDIKALWNTMDISYDDFIRTTEKRHT
                     DIIQKIFTKLYEQGDIYKGEYEGRYCTPCESFWTESQLLEGNKCPDCGRETYLVKEES
                     YFFRLSKYEDRLKELFKDDSFCFPAARKNEMVANFLDKGLEDLSVTRTTFDWGIKVPF
                     DEKHVIYVWVDALCNYITALGYMTDNDEEFKKYWPANVQIVGKEIVRFHTIIWPALLM
                     ALGLEVPKQVFGHGWILFADDKMSKSKGNVVYPEPIIERYGIDTLKYFLLREFAFGQD
                     GSYTHRNFVTRINYDLANDLGNLISRTVAMVEKYNNGIIPTAKVSTDFDADLKEQAVS
                     TRENFEAEMDKMQFHEALESVWKLVRRTNKYIDETMPWALAKDETKKDELDTVLYNLC
                     ESIRIIATLINPIMNETANKIYEHIGIKGQDDITTWESTKTFGLIGENVKVFKGEPLF
                     PRLDVEKEIEELTKMFSGKPPVEEKPLEHKEEITIDDLDKIELRVGKIISCEKHPKAN
                     KLLVSQVKIGPETRQIVSGIAEYYKPEDLVGKEVTVVCNLKPVKLRGVESQGMILAAG
                     DDGDPYVLPFTQGAKDGCEVR"
     gene            12597..13367
                     /locus_tag="PC700_000014"
     CDS             12597..13367
                     /locus_tag="PC700_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDF2579349.1"
                     /translation="MLFDSHAHLNDESFDEDRDELIGSLKDKGVDLVVNPGADIETSI
                     TAIELAKKYDFIYSAVGVHPHDVSKLDDTAIETLRKLATENEKVVAIGEIGLDYYYDY
                     SPREEQKEWFKKQIELANELKLPIIIHDRDAHGDTFEIIKNTKNPEIGCVLHCYSGNV
                     ELAREYVKMGCYISIPGTVTFKNNKKTREVVREIPLERLFIETDSPYMSPEPHRGKRN
                     NPSQVSFVADKIAQEKGICYEEVCRVTKENAKKFFNIK"
     gene            13704..14135
                     /gene="phnG"
                     /locus_tag="PC700_000015"
     CDS             13704..14135
                     /gene="phnG"
                     /locus_tag="PC700_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HDF2579350.1"
                     /translation="MYTVDSTKILINMNLEKLEKIYSMIKDTSSISIIKEPNLATVMV
                     RANESVKNTTFNLGEILVTECSVKVDESLGYGIVSENNNKKAIYLAVIDAVLHSNNSK
                     FDELKNYINKSVYEENLRYEQEIIDEFSLINKTKVQFNAMD"
     gene            14150..14725
                     /gene="phnH"
                     /locus_tag="PC700_000016"
     CDS             14150..14725
                     /gene="phnH"
                     /locus_tag="PC700_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HDF2579351.1"
                     /translation="MVNYTQEIYRKMLDSASFPGKLNNIDNINIENNTKLLNGAIGIA
                     ITLLDQEVTFYIENYTQKDIKNIRALTVSNQIDYRKSDYIFLDINSEVDILQIKIGSF
                     EYPDESATIVHQVNDLSTYSQTKYIKLDLSGPGIKSKNSIYINGIREEFIERLSTINK
                     DYPIGIDLILVDKKGEIAFIPRSSKLSWEVL"
     gene            14728..15813
                     /locus_tag="PC700_000017"
     CDS             14728..15813
                     /locus_tag="PC700_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HDF2579352.1"
                     /translation="MAYVAVKGGEEAILQAKNILEFYRVKGDSLPIDVKQIEEQMSYA
                     VDKVMSEGSLYNKELSALAIKQAQGDILEASFYVRALRSTMPRITYSEPINTEEMRIK
                     RRISATFKDIPGGQYLGATRDYENRILNVELLEEDEKKNRDFRDSFINELETVDEIPE
                     FTKISNIFRKENLLQEEVCENKLEEVIDDITRNSLRFPVSRSAKLQALTRGESGVVSG
                     FAYAIVRGFGDEHPYISELRTGNVFIKVKHPIYEDEEIIIGDMLVTECEIVSKASQNK
                     TENSKTNSEDKNKMSLGYGLCMGNNENKAISMAILDKSLEPNSEKLAQDEEFVLLHID
                     GIDSLGFISHFKLPHYVDFKADVERIK"
     gene            15833..16702
                     /locus_tag="PC700_000018"
     CDS             15833..16702
                     /locus_tag="PC700_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HDF2579353.1"
                     /translation="MIYNYGFLDENTKKEIRRKILKAISIPGYQVPFGSRELPIAKGW
                     GTGGLQLTLSLIGKNDVVKVIDQGSDDSTNACNIRSFIASVSNVETTKDTLEATLIQT
                     RHRIPEEKLKSNQILIFQVPIPETLRIVEPSEVETRRMHSEEDYSRMWVYLYEDIVRF
                     NDISIAVEYPCKVNDRYLMNPSPIPRFDIKKLNMSDNLFLFGAGREKRIYAIPPYTKV
                     EPLEFEDYKFEEEKFEGKYCSLCNSTNTFLDEVYDSDTNEKYYSCSDTSYCEKVRLKN
                     NSIDVTIGGTWNE"
     gene            16695..17561
                     /locus_tag="PC700_000019"
     CDS             16695..17561
                     /locus_tag="PC700_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2579354.1"
                     /translation="MSKINVSKRLENIDKQVLIGRNINLIYGDGCEKCFESTGYESNN
                     ICKYCGSIVACNNVNLELYEGEVLGIVGESGSGKSTLLKILFFQEHANSGEVYISHYN
                     KDTNILELSDQKKRYIKNHFMGIVYQNPHLGLNLDFSSGGNIAEKLLMANLYNVEKIR
                     DRGKELLIKTNIPVERIDHKPKYFSGGMQQRVQIAKALSNNPPILLLDEVTTGLDVSV
                     QAEVLDLIREIQRELKISMIVVSHDFDVIKMLADRTIVMKNGSVIESGLTDQIMEDPQ
                     HPYTQQLINSLL"
     gene            17602..18315
                     /locus_tag="PC700_000020"
     CDS             17602..18315
                     /locus_tag="PC700_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2579355.1"
                     /translation="MKETILEVNNFSKDFELHALNKTITACSNISFEVSKGEFLGIIG
                     KSGAGKSTILKSIYKTYIPTTGEIIFNSEIYGNVDLAKIGDREMINLRKKEIGYVSQF
                     LKTLPRITAIELVVHSLIESGFEKDCSYDMAKDILNQFEIKENLWDAYPNNFSGGEKL
                     RLNLAQAMVKKPRLLLLDEPTASLDNQSKIYVKEKMLELKSQGTTMIGIFHDIEFMET
                     VIDKTFTMAKGTISESGVA"
     gene            18316..19485
                     /locus_tag="PC700_000021"
     CDS             18316..19485
                     /locus_tag="PC700_000021"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HDF2579356.1"
                     /translation="MIKLIHNANIVLEDMILENGHLIIEGDTIKKVSNDRIDYAFDFN
                     EIDEIIDAKNLYVIPGIIDIHSDAIEKEIEPRPSTLLPFNMAFYELDKKLPANGITTV
                     YHSISLGDGVGVRSIDNSLKMIKNIDSYKNIDSKSINHKVHLRYEVLYYEGLEKVLEL
                     LDENKIDYLSIMDHSPGQGQYTNPTFYKEYATKVWGVTENYVDTWLDDLVNLHDNLDW
                     NKIANIIGIAKTKNINVASHDDDTLEKMDFIKNIGINVSEFPITLEVAKHSKKLGVLT
                     CLGAPNIVRGKSHNNNLKAMDAILEDCCDIVCSDYLPSAMIKSMCIVAERINNLNKAV
                     SLFTSNPAKAVGIYDERGSIKENKKADLVLVDIDSEYPKVLNTIVNGKTVYKREV"
     gene            19489..20325
                     /locus_tag="PC700_000022"
     CDS             19489..20325
                     /locus_tag="PC700_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDF2579357.1"
                     /translation="MLTELHCHTSISDNNFTTEEIIKKAAEKNINILAITNHDTLSGL
                     DEALVLGEHYGITIIPGIEISAYDYKNRKRVHILGYNIDLNSQEIKNLCNPMVCDRHK
                     ASVKMVNKIIDLGYKISLEDVKKYSSKTGIFKQHIMKALIDKGYTDNIYSSLYKELFH
                     RGNGKVYVSLKYVDYRDAIKAIKNSGGICVLAHPGQMDNFSAIEEMVKVGLDGIEVYH
                     PSHNKELEKESLSYAKKYNLVVTGGSDYHGFYSQHNYELGSKSLNKEDLFKFRLALGG
                     VL"
     gene            20326..21321
                     /locus_tag="PC700_000023"
     CDS             20326..21321
                     /locus_tag="PC700_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2579358.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHGGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSNIDKKVKEVLDVVELSGYEKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIRSLHRKLGITTIMVTHDQEEALCLADKMVVMNRGEIIQVGTP
                     KEVYKNPETPFVADFIGTINFIDDGINKIAIRPEDIKVESNRDSKDKDIKVGEILDIE
                     FRGFNYRITVEYRSKQMKLDVVSKVAEQMKLCIGSKINFKIPKEGIVQYKSEGCA"
     gene            21339..23027
                     /locus_tag="PC700_000024"
     CDS             21339..23027
                     /locus_tag="PC700_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HDF2579359.1"
                     /translation="MITSKNKRRISDSTIQKYFLIFSVIVLIVFILFPLVSLLKNAFM
                     DTSGNFIGISNFSKYVENPSLIASFKNSIFVSTVSSLISLCLAFIYSYAITRTNIKFK
                     DFFKTMGMFPLFAPTMLYGISLIYLFGNKGIFTTMGLEVPLYGPLGIIISQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFCKITIPGIKYSLLSSFFVSFILAFTDF
                     GAAKVVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTLDKMASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIVCSFITLLIFILIFVSVIGSVIKLWPYNLSFTMEHY
                     TFKGILGSGIKTYLSSLKISTLTAIFGTIFVFFSAYIIEKVDKFPKLRQTAYLLSMIP
                     MALPGLVLGISYITFFNSASNPLNFLYGSTAILVIVNVVHFYSVCFITSNSSLKMLDK
                     EYELVAKSINIPFYKVFFNITVPMSITSILEIVVYYFVNSMVTVSALIFLYIPQTQTA
                     SISILKLDEIGYIGPSAAMAVLVLLTNIIVRLLYEFVTKKLKNNTQKWQQKDVA"
     gene            23039..24106
                     /locus_tag="PC700_000025"
     CDS             23039..24106
                     /locus_tag="PC700_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2579360.1"
                     /translation="MKFKKLVSLGVLSTLLLSVITGCSTDSSGSDSKSDSKTKGDLTV
                     YTAIEEDSIEPYLATFKEKYPDIKLNIVRASTGDITARLLAEKDNPQADVVWGVAATS
                     LLVADDQGMLEPYAPKGSEEIESAFKDDKEVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPSSSGTGYLTVSALIQIMGEEKAWQYMDKLHENIGVYT
                     QSGSAPATSAASGEYPIGISFGYRGIKLKEEGYPVEVVFPKEGSGWDIEANALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNYAVTTISTGNPIPEGFPKKPLEQMIDNDLKSAAK
                     NREDILNKWISKYDGKTEKES"
     gene            24592..24708
                     /locus_tag="PC700_000026"
     CDS             24592..24708
                     /locus_tag="PC700_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein"
                     /protein_id="HDF2579361.1"
                     /translation="MDDDINMIAIRPEDIRVEFNRDFKEIIDLKLRPYRYKN"
     gene            24872..25939
                     /locus_tag="PC700_000027"
     CDS             24872..25939
                     /locus_tag="PC700_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2579362.1"
                     /translation="MKFKKLVSLGILSTLLLSVITGCSNVSSGSNLKSNNKTKGELTV
                     YTAIEEDSIDTYLATFKEKYPDIKLNVLRLSVGGITARLLAEKDNPKADVLWGVAATS
                     LIILDDKEMLEPYAPKGFDKVNPAFKDDKKVPSWVGIDAWMTGITVNTKELKAKNISA
                     PNSYEDLIKPEYKDLISMPSPASSGTGYLTVSELIQIMGEEKAWQYMDKLHKNIGVYT
                     QSGSAPAILAASGEYPIGISFGNRGIKLKGEGYPVEVVFPKEGSGWEIESNALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNFAVTTIDTGNPVPEGFPKNPLEQMIDNDLKSAAR
                     NREAILNKWIEKYDGKTEKES"
     gene            26131..26661
                     /gene="rnmV"
                     /locus_tag="PC700_000028"
     CDS             26131..26661
                     /gene="rnmV"
                     /locus_tag="PC700_000028"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HDF2579363.1"
                     /translation="MIKEIIVVEGRDDVTAVKRAIDAELITTGGFGFPKGVMQRIKAA
                     NERRGVIIFTDPDFAGEKIRKKIASEVPGCKHAFLPREEAKKDGDIGIENATPQSIIA
                     ALNKVRTENTEKRDEFKQVDLIRNGLIGNEEASLRRDALGKILGIGYGNAKQFLNRLN
                     NYGVEREEFIDALKQI"
     gene            26832..27701
                     /gene="rsmA"
                     /locus_tag="PC700_000029"
     CDS             26832..27701
                     /gene="rsmA"
                     /locus_tag="PC700_000029"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDF2579364.1"
                     /translation="MDRLSSHNATKEVVQKYNFKFSKSLGQNFLIDSNIIDKILSGAR
                     ITRGDNIIEVGPGIGTLTREMGKIAEKVVAIEIDRNLIPILKDTLSDLDNTEVVNQDI
                     LKVDIQELVKDKLNGGPVKLVANLPYYITTPIVMKFLEEDIPVTDIVVMVQKEVADRM
                     NAIPGTKDYGALSIAVQYYCDTEIVAKAPRHMFIPQPNVDSTVIGLHVRDKRKYDVHN
                     EDIFFKTVKASFGQRRKTLLNSLGGLGFLNKDEIREILKEANIDEKRRGETLSIEEFS
                     VLSNIINTKVSSK"
     gene            27767..29038
                     /locus_tag="PC700_000030"
     CDS             27767..29038
                     /locus_tag="PC700_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2579365.1"
                     /translation="MGSKRKFKRDYIILEAKDINFRYKERVLPKAFAKVEVNDEKAII
                     ALYVENLKYVKDGYKAVAIKDDHGLIDLGNIILNEQGRGEFVLDLEDSDDEIKGIALL
                     YERNIPLIGFKGNKLENYEDILFQEVEEEEYEEYEEYEELEEDEEIEDNEESDRDEDS
                     EEEYIIEEYDEDEYEDYWEDDEDIEEGELKARTEVEDSGDEEIEEFNNYKEIENYNRV
                     KVQEEYIDIEYDEEEEEEEEVFEVNREEDRFVSSKPTKSKKVQKDQNNIEKIKRANGV
                     SQGESGNKQTSTLLMPRQIKKGLKLFKEVKPFVTDYIDKTRWWKIEINPTTLCGYTMP
                     YLGYLNVLNYTMYSDAVLHSYKYRHYLFGVQYDEYNKRKHYIYAIPGNRNEQPDNGST
                     GFLRYQACDNRTNSLGYWLCFIDSRQRNIVR"
     gene            29189..30304
                     /locus_tag="PC700_000031"
     CDS             29189..30304
                     /locus_tag="PC700_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF2579366.1"
                     /translation="MINEQRILNEFLELVQIDSLSLKEGNVAKVLVKKLEEIGCSVVI
                     DNAGEKANGETGNIIATLKGNKEGKKILFSSHMDTVTPGIGVKPIVDEANGIIKSDGT
                     TILGSDDKAGIAAILEGLRYINENNIEHTDIQVVFSICEECGLVGAKNLDYGKIDSEY
                     AFILDSGGSPGEIIVKAPAQDVINVKILGKTAHAGLEPEAGISAIMVAARAIENMNLL
                     RIDEETTANIGIINGGTATNIVTGEVNIVAEARSLKENKLDVQTKHMVETFEKAAKDF
                     GAQIEIDVNRAYTPIDVSEDSEIIKLAKKAFSNLGIEGHTESTGGGSDTNILSKNGIE
                     AITLGIGMKNAHTLSEHIAIKDLYDSAKMVVEIIKEA"
     gene            30490..31386
                     /locus_tag="PC700_000032"
     CDS             30490..31386
                     /locus_tag="PC700_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDF2579367.1"
                     /translation="METRYEEANKITIQSILWNVVLTIIKVIAGVIGNSSAMIADGLH
                     SASDIISSIGVLIGNYVSSRPGDREHNYGHEKAETLVSFVLSILLIFVSITIGIEAIK
                     SLFNLDALSVPSILPLVVSVISILIKEYQYRITIKVAKKINSPALKADAWHHRSDALS
                     SVAAFIGIGGSILGFKPLDPIASIVVAIFVAKVGISILISSVNELMDVSVDEEEIKEL
                     KFIVADTEGVKNLGDIKTRKHGAMAYVDLTICVDENLTVKQGHDIATKLEKHIIKHME
                     FVKGITVHVEPCTNCQGNKCNN"
     gene            31456..32253
                     /locus_tag="PC700_000033"
     CDS             31456..32253
                     /locus_tag="PC700_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2579368.1"
                     /translation="MSKKRKLKKKAAILIGVVVFFIIYFISFRIGINMEKDKSEVPKK
                     VEKEEEDNRSLRTQIKEKKKVYISDKNIENIKIEEDMWDEIKLFFSDFKKVRNTTSSY
                     EAVYEGYSDDGVKFSTDLNFFRVYTVNKEEYYKVPVSSKDAFKKLLDESIYTSFDLLS
                     HYKDWKEVTITHGKEIKKVHRWKFDDLAHKMISKRVVGKVQPEKSKERSDYNFTINIK
                     GDNFDVKVDTMGKDYVKVTSKKNVSYYEVHTSLFDYLKNEIFEIGKK"
     gene            complement(32309..33661)
                     /locus_tag="PC700_000034"
     CDS             complement(32309..33661)
                     /locus_tag="PC700_000034"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDF2579369.1"
                     /translation="MNIHFIGIGGISMSALAEICINKGYQVSGSDSNESYLLDKLRDQ
                     GATIYIGQKKDNISDDVNMVVYTAAVHPDNEELVAAKEKNKLVMNRATFLGQIMREYK
                     NSIAVSGTHGKTSTTSMLSTIFEYADLDPTILVGGNLSMIGGNVKIGNSNHFITEACE
                     YVDSFLNFNPKISIVLNVEEDHLDYFSGIDEIKASFNKFGKLLPPEGYFIINGDDENI
                     DDILYDVKATIIKYGRDSDNDAVIKDIHFDNSGHGIFRIEYEGRDLGEFELSVYGLHN
                     IYNASSAIMAALVSGIDLETIRKNIKIYKGVGRRFETKGYYKNALVVDDYAHHPTELK
                     ATLAAAKKLKKSTLWCIFQPHTYTRTKSLLGEFSEAFYAADKVIITDIYAAREKDPGD
                     IHSKDLVEKLYQNNVDAIYIKEFEDIVKYLRENVKDNDLVITAGAGPIYKVADLLVEK
                     "
     gene            33880..34758
                     /gene="purR"
                     /locus_tag="PC700_000035"
     CDS             33880..34758
                     /gene="purR"
                     /locus_tag="PC700_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HDF2579370.1"
                     /translation="MKFKRTERIGAIVKILSDNPNKIYTLSYFTNQFNAAKSTISEDL
                     LVVKNVFEKLHLGKVITISGAAGGVKYIPKTSIAENAEFLMELCERICDKSRILSGGF
                     LYLIDLIYDPTIAAKIGKIFASNIEYVDADYVVTMETKGIPMALMTAKAMNLPLVIIR
                     KDIKVSEGPTLSMTYVSGNSSKVESMSLPRKALKPDSKVIIIDDFMRGGGTIKGMVDL
                     MNEFGAEVIGTGVFISTTNPSEKMVKDYISLIQLDVNGDKIVVEPNLKTFKDEYRNED
                     LDEEEDSEFEFEIDED"
     gene            34914..35192
                     /gene="spoVG"
                     /locus_tag="PC700_000036"
     CDS             34914..35192
                     /gene="spoVG"
                     /locus_tag="PC700_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HDF2579371.1"
                     /translation="MKITDVRVRKLTEEGKMKCIVSITFDNLFVVHDIKVIEGHNGLF
                     IAMPSRKVGEGNFRDIAHPINAEMRQVLEDAVLQAYHEALVQWEVAAE"
     gene            35402..36781
                     /gene="glmU"
                     /locus_tag="PC700_000037"
     CDS             35402..36781
                     /gene="glmU"
                     /locus_tag="PC700_000037"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDF2579372.1"
                     /translation="MNFKAIILAAGKGTRMKSKYPKVIHKVCGKEMVNHIIDVSKKSG
                     VKDTVVILGHEAEVVKEKLAEEIIIAMQTEQLGTGHAVKMAKEYINDEDTIVVLCGDT
                     PLIKEETLKRLFEYHIENKYHATVLTTRVGNPTGYGRIIRDKKGDLLKIVEQKDANSE
                     EKMISEINSGIYCFNGKSLREALDLLNNNNSQGEYYLTDTAKIMRDKGLKVGAFAGST
                     IEELMGVNSRVELSKAEEIMRRRINESHMVNGVTIIDTNSTYIESDVMIGNDTIIYPG
                     VMLQGKTRIGSDCIIGMNSSITNSEIGDGTEIKNSTIIDSKVGENSNVGPYAYLRPKS
                     DLGNNVKIGDFVEVKNAIIEDGSKASHLSYIGDAHVGKNVNIGCGVVFVNYDGKNKFK
                     SVVKDNAFIGSNSNLVAPVVVEEKGYIATGSTITHDVPDGALAIARERQVIKEGWVEK
                     KNQKDDQSK"
     gene            36876..37826
                     /locus_tag="PC700_000038"
     CDS             36876..37826
                     /locus_tag="PC700_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HDF2579373.1"
                     /translation="MNTSGSEIKIIAGNSSKELAQKIADYIGVSVLDCEVGTFSDGEI
                     CVNMNETVRGCDVFVVQSTNSPVNDNLMELLILIDALKRASAGRITAVIPYYGYARQD
                     RKAKARDPITAKLVANLITAAGADRVLTMDLHAAQIQGYFDIPLDHLLGGTILANYFN
                     EKKIEDLVVVSPDLGSVTRSRKFANTLNGEVPIAIIDKRRPKANVCEVMNLIGDVKGK
                     NVILLDDMIDTAGTIVNAANALKEFGAKDVYACCTHGVLSGPAIERIANSEISELIVL
                     DTIQLPEEKRIDKIKIKTVAPLFGDAIRMIFSNESVSKLF"
     regulatory      38666..38751
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            38913..39434
                     /locus_tag="PC700_000039"
     CDS             38913..39434
                     /locus_tag="PC700_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF2579374.1"
                     /translation="MKLKKNKKGFTLVELLVVIAIIGILAVVAVPALFSNINKAKVAS
                     VESDYSSVKSAALSYYSDTNKIPVTPDGQTGLSVLETYMESLPDKADIGGKYKLIKVG
                     NKLVLQIGTNDEGVTLTEAQSAKLLSDIGENKIYTSVTADNLGNPLTSNTKVDNKVLY
                     IVLIDNTVMDSTK"
     gene            39585..41261
                     /locus_tag="PC700_000040"
     CDS             39585..41261
                     /locus_tag="PC700_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HDF2579375.1"
                     /translation="MAKKVRIGDKLVEKGYITEDQLKWALSEQKNSGKRLGEFLVQEG
                     LIDSNLLISVLKELLDIESIFLEGTEIDTLATKMVPENICKRYTVFPFKIDGNKICLA
                     MSDPQDREAVQDVRRMSGKDVEIFISSTEDINKAIGHAYAHSEINKAMTEYNKNRTGG
                     VRETVILEEDVNAAPIVRLVNNILENAVRMEASDIHIEQSENYMRVRFRIDGMLREYM
                     RMNSAPYKAVISRIKIMSDINISEKRIPQDGRIYLKVDNKPIDFRVSTMPTNRDEKIA
                     MRVLDKSNFMVSKEVLGIDEHGSKIYDELINTPYGLILVVGPTGSGKTTTLYSMLNQL
                     NTENRNLLTIEDPIEYELPGVNQSQINEKAGLTFASGLRAFMRQDPDIIMVGEIRDTE
                     TAEIAIRASLTGHLVLSTLHANTAVGAISRLLDMDVESFLITSSVLGVISQRLTRKIC
                     EHCKVSYEADIGEKKALGIDVNESVTIYRGKGCERCNNTGYKGRLGIFEMLEITPEIK
                     ELIDSSANQREILKMARKQGMVSLKEDIVKKVLNGKTTVEEMIRIILMTD"
     gene            41273..42481
                     /locus_tag="PC700_000041"
     CDS             41273..42481
                     /locus_tag="PC700_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDF2579376.1"
                     /translation="MKSFKYTVIKEDGKKQTDVIEANDFNEARNLLRKRNLRVIEIKE
                     SKNKNIRLSSRKRKKDLGADQISHFCRQFAIIVSSGINSISGLETLARRSTNITLREE
                     INRIVAEIKVGSTIADSMLSSKSKFPKLLGAMVATGEATGKLDEVLKSMASFYASEHR
                     VKQKLRNAATYPLIVLISSFIMIFIFTTFMVPKMINSITTVGATLPLITRIVMGFGMF
                     MKKFWIIVLLVIILFIYQFKKYLKTSVGRAHKDRVINKIPVLGKGINCMVATRFSRAL
                     YLFVSTGYPLVQGLDYIIDSVNNTMAEKILASAKDGITRGEGLAENLERYTYFDSVLV
                     QMIAIGEQTGELENISRQMAEFYEYESEIYLNRMASMIEPVLIIAVGIIVAILVVSIF
                     MPMLSIYDAM"
     gene            42496..44208
                     /locus_tag="PC700_000042"
     CDS             42496..44208
                     /locus_tag="PC700_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HDF2579377.1"
                     /translation="MNKVYVSYYGDSISIVEGRYKHDKDKFYVKNFNVFSIEDIAQDF
                     SKEKYALLRYALENVKLKSRHVVFCLNTRDVILKPQKLPNMDKKDLDGFMKLEMDEMM
                     ALESDEYVFSYEVSSETHDNSDGDESSLNLIIGAIQKEEVNAIVEIMHEFDLVLDRID
                     TLSTAYLRMLKNLDYNDIMVTNISDNSTIINIYKKDTLFISDNIPIKITAKDMDMQLL
                     NVVEESKGLMNYYSSRNFGKVTDTILILGKRYSNKLIYETFSSAFSSYVSQGLTEIMD
                     ISDMITGDIDEDDINSIVELLGSMIEPKGKRDFENINFLPMDILRKQIKAKQMKNILK
                     IAPIVILILAIPYLCLIFSNNLANKELENVNGEIRNIESAYYQIGNIEKMIQSKSEEI
                     KVYDMLIGKKVKWENLLDDIDKNTPSKVELTSLSTTYQNLGTNNKADNSKSNKTTTDK
                     SSNNETKSSTKSDSNGDKTDNDLNEDKTTNVSEALTGNSETNSNNTDKDDKTPLYDKI
                     PNVINIEGNAKDSSYVGQFLYKLKGLYYFKDVKLHSIKSNAEDSNYTFSMTLYLKEGV
                     LTNE"
     gene            44201..45109
                     /gene="pilO"
                     /locus_tag="PC700_000043"
     CDS             44201..45109
                     /gene="pilO"
                     /locus_tag="PC700_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HDF2579378.1"
                     /translation="MNKDIFKKDIKDIFKMEVSLKGILIKVIILLAILSYGYFGLYPQ
                     YQKYIATKNSVKQATEKLNIYKEKTALMPKYEKQLEEVESELKEKSKNLLYDMQDGLF
                     LIGLDKKMKAYNIDLTSYDVGNVKEYEHFYGISTTISVRGDYRNVKKLMAYLEEQKNV
                     TQILDYTMTSYIEPEKEEEETTNNKDLIKNVNINKDTKIYWVEGENKWHLYKDCPLIK
                     EGEILKNDKLLDRSTNYLCNTCVLRAATEKEAKKEVVAPKAEGVVDATFKFIMYTKEN
                     TTMILDTDNPSSWRPGKYNPFKSSAK"
     gene            45136..45702
                     /locus_tag="PC700_000044"
     CDS             45136..45702
                     /locus_tag="PC700_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF2579379.1"
                     /translation="MSLYKNNEKGLTLLEVIIAVFILTIVLSISYKVFNGITSAVKKQ
                     QIITDAQVNINLINKYLNRDLENCKELTKTGSGNNYEYNIEMPDNVVKYEVSIETKKN
                     TEVYSVTRIQNNTIDTENEVREEIIYNQPLVQNNKEMKETPFKIEKQTGKSIYTVSIY
                     YNESVQESHKNSNLNNKTYTFDVMSRIG"
     gene            45739..46266
                     /locus_tag="PC700_000045"
     CDS             45739..46266
                     /locus_tag="PC700_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF2579380.1"
                     /translation="MSKKNSERGFSLIEVLVAMAIMGIVLFAFFNIINTNNKANTKND
                     TDITSLNYVQSEIENLREKIKSGEFDFDSLDKLEDGTVVYEKLIDKSKKVVYDKVLSE
                     GDVSLYDTPYEKITTIKDEDGNLIDKGNITNKIKTIVEDKSGQIYKIAVTGKSMNDYS
                     SKKEVKIVTEIFKDK"
     gene            46287..47825
                     /locus_tag="PC700_000046"
     CDS             46287..47825
                     /locus_tag="PC700_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2579381.1"
                     /translation="MRKWNKFKSERGAALVLVLIVVALLSIVGLIFSNQIANRIKSTK
                     TTNEGIQAKYLAETCVENSIDKAYEKLYDELEKMDNEFKSENQEKSISRSKLRNISDE
                     DFNNQDEKNIEAERLGYMNNINFYLNKASSDLEKASMELKKLYDLDMLDYRDIEYVDA
                     NIISHRDSILEICKNYTSGDISKINEYILKEDIDSTTLIEAKLVNNDILLKMFLEENK
                     IENEHLNSAFSHTYKALDNISLAMQNMIEYRHTFHIDEPKVEVSNGIPDSQQYYELIQ
                     NPIINSMEYIWNSKWDTLENLLEILPNQTQGFNSLRVHLRNNVRKFEKLSDNISSGKK
                     NTAKNFLKYKELLYEISDQCNQLKSMSYEKIPVKYDNMALITTFDYIQNELLAEIKCR
                     LKELKPQEIDKTEGITIKIPFYKADYDMTKEGWPKLKENGSGAELSLMVTGDKDGIKE
                     VEVTDGKKNIIGLGVEENSNSKYKVDAIVNFNLNIDTNVVGNYDIKDKILINHDISSY
                     KKVN"
     gene            47925..49007
                     /locus_tag="PC700_000047"
     CDS             47925..49007
                     /locus_tag="PC700_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HDF2579382.1"
                     /translation="MYIYDLLEQGIRLNASDIHITVGTNPVARVKGGFVKLSEQILTS
                     EVTMQMAKDIAGESIFKVIEEHGEADFSASLKTGERFRVNAYRQKGNYAIAIRTITAE
                     IPTFEKLGLPESIKSFTEKHKGLVLVTGPTGSGKSTTLASMINIINEKQQKHIITLED
                     PIEYVHHHKQSLVNQREVGTDTESFHSALRAILRQDPDVILIGEMRDPETVSIALTAA
                     ETGHLVFSTLHTVGAAKTIDRIVDMFQPSQQQQIKTQLSTVCEGVVSQQLLPTADGKG
                     RIAAIELMFATPAIKNLIREGKTYQIPNMIQTGVKVGMKTMDQDLMELYKNGKITKDM
                     ALSRCTDQEFMTRMIGGVNYNGYYNR"
     gene            48988..49770
                     /locus_tag="PC700_000048"
     CDS             48988..49770
                     /locus_tag="PC700_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDF2579383.1"
                     /translation="MDIIIVNLYVFIVGIVFGSFFNVCIFRIPEGISISRPPSHCMSC
                     NTRLKPIDLIPVLSYLMSGRKCRYCYEKISSRYAIVELITGILFLTVFMLYGISTSTI
                     YYLVLVSLLIIITFIDIDHFIIPDKILIFGAIFSMIFNLLFKEIPVKDSILGVFICGG
                     SVWIIVLLIEFVIKKECMGGGDIKLFAMLGLYMGVKNGLLTALLSVYVGAVYGICVII
                     ISRIKGKEYNSVIPYGPFISIGALITILCGKQLLELYFSMVI"
     gene            49872..50522
                     /locus_tag="PC700_000049"
     CDS             49872..50522
                     /locus_tag="PC700_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDF2579384.1"
                     /translation="MEYIIIISLFFAHLINKSLCVFCDREKKNLYNTFFIYIITVVLS
                     VLIYLKFGISVECVQYMVLIPFLIIISIVDYYTIYVYDITIVSGIIVQGIIFLAFFNI
                     AQFDYINHIFGLLIGFIIPYIIAKLTKGLGYGDVGLYSLCCFALGNNYGFYVIVLSFI
                     LAFLFFIILYITKIFKDVDIRKQIPFTPFISLSTILIIFTEYNIFNFYYNLLYKFI"
     gene            50536..51096
                     /locus_tag="PC700_000050"
     CDS             50536..51096
                     /locus_tag="PC700_000050"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDF2579385.1"
                     /translation="MYVVVGLGNPGKKYEKTRHNVGFDVIDILAKEYNISVTKIKHKA
                     LIGEGRVGTEKVLLVKPQTYMNLSGETLIDIYKYYKVDLSNIVVVYDDIDLEVGKIRI
                     RKKGSGGTHNGMKSITKCLGSNDFPRVRVGVSKPEAGQDLADFVLSRFRKEESDNINE
                     ALEKAADAIDSIIRENIDMSMNKYNG"
     gene            51113..54499
                     /gene="mfd"
                     /locus_tag="PC700_000051"
     CDS             51113..54499
                     /gene="mfd"
                     /locus_tag="PC700_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDF2579386.1"
                     /translation="MNDVFLYPLQNSKEYKDIINCIKNTKGSLLVNGLLPVQKPHISY
                     SIFNDLSRQMIFITSSDLEAKKVYEDLSFYMEDKVEYLGFQDIYFYHLDAKDRNEEAK
                     KLKVLLKLANKEKIILVTSIEAVLRKYIPKQVLLDSVSHYKVGDSLDLEKLTEKLVSL
                     GYERVSKIEGFGQFSIRGGIIDVFSLEYTNPIRMELFDDEIDSIRTFDVYSQKSIDKL
                     QQFSITPSREFIYPEKTTDALVKLKKETTKNTDEGTFQNIDYISSKTYFEGAENYIDY
                     IYPEENKSIFTYLADDAIVFINDITRLKERCENYINEFRENYKLNLERGLAIKNQGKL
                     LYHYTDLEYLVKDKSVVLNSLLPKSINNFSIKSIINFESREVPTFNGKVDLLVEELNR
                     LKYNGYKIILATNTLERANKLGKDLLDKGLETTISKDRDIEIKSSQVIIVPAHINSGF
                     QYKSIKFVVITDNEMIGVYKRASKTSNKKVKKGKKIESFLDLSVGDYVVHENSGVGRY
                     TGIEQITVNAIKKDYMKIVYQGGDNLYVPIDQMDKVQKYIGAEVEKVKLNKLGTNEWT
                     KAKAKVKKEIEDMTKDLIELYAKREKIQGYKFSKDTPWQAEFESLFPYQETEDQLKAI
                     EETKKDMESSKVMDRLVCGDVGYGKTEVAIRSIFKACMDQKQVAVLVPTTILAQQHYN
                     TFKERFENYPLRVEVLSRFKTPKQQKQIIEDAKKGLVDILIGTHRIISKDINLPNLGL
                     VVIDEEQRFGVKHKEALKKIKSTVDVLTLSATPIPRTLHMSLSGIRDMSVIEEPPQER
                     HPVITYVTESKESVIQDEIERELSRGGQVFFVYNRVEHIEEMASMIQKLVPDARVAVA
                     HGRMTSKSLENIILGFLNKDYDVLVCTTIIETGMDISNANTMIIYDADKMGLAQLYQL
                     RGRVGRSSRQGYAYLLYEKDKTLSEIAEKRLKAIREFTEFGSGFKIAMRDLEIRGAGN
                     ILGSQQHGHMAVIGYDLYVKMLNDAIKKVKGEPIVEEIDVEIDLSVNAYIPDNYIKDE
                     LIKIEMYKKIASIENKEDMLDIQEELEDRFSDIPKPVQTLLTIAYIKSLCKILKIEKI
                     RQLKDEILLVPITKYRTKQKIGYNIVTELEELLEKMCKVK"
     gene            54530..55525
                     /locus_tag="PC700_000052"
     CDS             54530..55525
                     /locus_tag="PC700_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2579387.1"
                     /translation="MKKVITLVIAMILVVSVTACSSSKGETVATVEGTKISSDEFKKT
                     IALYKDSMEQTYGKDIWDKEVEKGVKYKDKFKDLILDQLITTEVIYSQAKKDNLLPKK
                     EDVEKSFKELKDAMGKDEKYKEQLKKLGIDDEFLRDQQEKDLAMQNYQSNFAKKTKIS
                     DEEMKKYYDTHKDEFKKDEVEASHILLKTVDDNNKPLSDKEKAEAKKKAEEALKEVKS
                     GEDFAKVAKKYSQDTSASDGGKLGFFSRGQMVAEFEDAAFSMKKGEVSDLVETQYGYH
                     IIKVTDRINEQTSFEDAKETIKDQLLKNKYQEQIEKLTKEAKVEKDEKVINKITI"
     gene            55649..56188
                     /gene="spoVT"
                     /locus_tag="PC700_000053"
     CDS             55649..56188
                     /gene="spoVT"
                     /locus_tag="PC700_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HDF2579388.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRKTLRIREGDPLEIFTAKDGEV
                     ILKKYSPIGELNEFSQEYAETLAEVLGYGVLITDLDSIIAVSKLPKKDYKEKSISDEL
                     EAIISERTVKYSKDKNILPLFKEDQKEYTSQVIMPIISASGDCIGSIVVVSKDKDTIA
                     ESEEKSLKIAANFLGKQVQ"
     gene            56308..57921
                     /locus_tag="PC700_000054"
     CDS             56308..57921
                     /locus_tag="PC700_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDF2579389.1"
                     /translation="MNNNKNKDSFLKGALILGLAGVIVKIMGGFFRIPLGNMIGSEGM
                     GYYQAAYPVYTLFLTLATAGFPTAVAKLVSEKVAIGNFKGANKIFKVSHTVLFITGII
                     SFCILFFGADYIVTNVMKNPGALYSMKAIAPALLFVPAMSAYRGYFQGRQDMTKIAVS
                     QVAEQFFRVVLGLTLAYLLMKSLGQTFGAAGAISGATIGSIASMLYLVFAYMLGRKER
                     RAEIDASQRFKDERVSYIFKKLLTVAIPITIGASVMPLVNMIDNVIVIRRLMEAGFTY
                     KVANSMFGQLTGMAMATINLPAVITTAMSMSLVPAISKAYALGNKSKARKDTKSAVKV
                     TLLIVLPCAFGMASLAIPIMGLLFPHEPSSVGTILFTLTPCVLFLGLIQTLTGIIQGM
                     GKPIVPVIALCVGMLCKIVISYTLTGIPDINVLGSAFGTVTAYFVAAMINLLYVKKHM
                     NVNFSKKEFIIKPFITVMTMFIMVKLSYGALVGFLGNSISTIIAICVGGIVYVVVILG
                     IGGIKKEEILTMPKGDKLYKLLKKVKLIR"
     gene            58028..59476
                     /gene="mazG"
                     /locus_tag="PC700_000055"
     CDS             58028..59476
                     /gene="mazG"
                     /locus_tag="PC700_000055"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HDF2579390.1"
                     /translation="MGKISIVGLGPGDYSLISQGVLESLINSPKVFFRTEKHPIIDKL
                     KETIQYTSLDYFYEKEENFDNVYLKISEFIIEESAKGDLVYAVPGHPRVAEKTVSIIE
                     RLANEKHIAVEILPSMSFVDAMFNYLAIDPSEGFKLVDAFEIENSYIDLDTSMIITQV
                     YDKFIASNVKLKLMEHYDYNQEVCIVNGAGVKDLESKKFIKLCELDRNENFFDYLTSL
                     YIPKSSKKMYNTVHDLEIIVNTLRSPSGCEWDKKQTHQSLKNSVIEEAYELCNAIDNN
                     DIDEMVEELGDVLLQVIFHCQIGNEEGYFDLKEVVNGICKKLIHRHPHIFSGAKLDMS
                     KFEKTWEELKKEEKGESTITDGLRRIPKHLPALMKASKVQHKAALVGFDWDNVEDVFK
                     KIEEEYQELLDEHRVGNIKYIKEELGDLLFSIVNLARFLDIDSGEALNCTTEKFINRF
                     DFIEKSAMNLNKKLEDMTLEEMDEFWNQAKKQ"
     gene            59565..59843
                     /locus_tag="PC700_000056"
     CDS             59565..59843
                     /locus_tag="PC700_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003153447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HDF2579391.1"
                     /translation="MNKAELVSKMAEKSGLTKKEAEAALNAFMSSVQDALVNNEKVQL
                     VGFGTFETRERAARQGRNPRDPEQVIDIPASKAPVFKAGKGLKDIING"
     gene            59915..60157
                     /locus_tag="PC700_000057"
     CDS             59915..60157
                     /locus_tag="PC700_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HDF2579392.1"
                     /translation="MRLDKFLKVSRIIKRRTVAKDACDKGIVTINGKVAKSSSEVNIG
                     DTLEIQFGEKKMKFKINEVKEHVLKNDAKEMYEILE"
     gene            60219..60473
                     /locus_tag="PC700_000058"
     CDS             60219..60473
                     /locus_tag="PC700_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HDF2579393.1"
                     /translation="MEQNITLKDRSKLVISGVEHIYSFNDKRVELKTSVGEMVIEGEN
                     LDMSKLSIDENIISIDGTINSMVYAKPKKPQESFLKKVFR"
     gene            60474..61007
                     /locus_tag="PC700_000059"
     CDS             60474..61007
                     /locus_tag="PC700_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2579394.1"
                     /translation="MIPFTQDVSIFYATIYGGILIGVLFDFYRGLRGNFKFINYFAII
                     FDVLFWFLATVIIFVTINLTEFFDLRYYHFVALFIGFILYYNTISKIVLSIINKIIRF
                     VRNSFKKVTHYIVSFLNNLYYVIIYSLHLLFDIIFYIPNIFIATRKSIKRKSNKKLKN
                     KKKSKPKKKKNKTKKRV"
     gene            61013..61297
                     /locus_tag="PC700_000060"
     CDS             61013..61297
                     /locus_tag="PC700_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HDF2579395.1"
                     /translation="MNLRKKFSGQIIVISLFLGISIFSMMTGFVFEYTKAKEYKKEIA
                     SLNKQLKKTEIQINSLKKDEKSYEGDLEDIARKRLNMVKPNETVYVDINR"
     gene            61410..62330
                     /locus_tag="PC700_000061"
     CDS             61410..62330
                     /locus_tag="PC700_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HDF2579396.1"
                     /translation="MKVGAIDIGTNSMRLLIADYRDGNFINRKKFVNTTRIGYGVDSK
                     GYIKEDAIKRNIKALKEFSDKCNDEKCEKIYCMGTSALRDSKNGDYFVKLAKEETGID
                     VDIVTGIEESNLGFKGVLEGIQGDDTIIVIDIGGGSTEFIVGDSEGIKFNKSENVGAL
                     RMTERFLKHDIIEDEEFEAMSSFIYDEIDKTLNHIKDRGLKTLIGIGGTITSLSAMNQ
                     ELEIYSMEKIHNSKIHKKEIEEILQKLIKMTLSDKKNLKGLQEKRADIITAGVKILNI
                     IMEKLEIEEITVSEYDNLEGLICQKSKKMY"
     gene            62420..64792
                     /gene="spoIIE"
                     /locus_tag="PC700_000062"
     CDS             62420..64792
                     /gene="spoIIE"
                     /locus_tag="PC700_000062"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HDF2579397.1"
                     /translation="MQRSVAVVKNKTVKPNVIKLISGYIDFTTAAFAVMGFLLSRSII
                     VDSVAPLGIAFFICASKIDKYKIPVFISTLLGILLSSNDFGNVAKYAVCLTIFMAMNK
                     KLKLSDSIAKVAFIGAIILLPISMGQAFFSNRYVYGLLIAGVEAIIMFISIYVFSYGI
                     GLMANVNNRMSVRTEEAISISLLLTFSIMGIGDISLFGISIRTILSTVLILLTSIVGG
                     ATMGASSGVIIGVASILNNMTSAVYMGIYSFSGLISGAFNKINKYFCILGYILSWTII
                     YLYTSGITSNMMQLRDILLGCLIVLVLPERLFNKIEKLIKSNVASNEIVYDYIMRSKN
                     LTNSRLNSIYKTYDDLADTFDKIREKDKVLDQRDIANVIDMIHNDECKSCSMRRMCWE
                     SRFNHTYTMIYNILEKIEEKGELSLNDIPKNFRKECMKPESIVKISNHYYKMFVLDYD
                     WSVKFSESRKLIANQIRSISKSIKSLSQDLEGDIMLDIEKEKNIYEQLERYDITVDKV
                     SYLTKSNNEFEISIEKKTCHDGCMCEDKIVNIISDLVGENMSVRKIGCHCLGGKCKAT
                     FVKSQKYKAVTEVSAMSRDGHILCGDNYTYMEINDGKYMMAISDGMGKGKKAYEESSA
                     TIDILEKMIDAKIKDEIVIDTINNMLLLKSSEEMFSTLDLGILDLKRGCLETIKMGAC
                     STYIKREDGEVDLISSSSLPVGILSDVKIDRKNVKVKEGDYVIMVSDGIVDAGRNNNL
                     GDNWLIYFLKNIETTNPKEISNLILDRALELQALQIEDDMTVLVTKICTK"
     gene            65143..67035
                     /gene="pepF"
                     /locus_tag="PC700_000063"
     CDS             65143..67035
                     /gene="pepF"
                     /locus_tag="PC700_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDF2579398.1"
                     /translation="MNRKLCKAIIPLFISIFCMLIISIKADQGYQKNNDRAIAVMNNN
                     DTEDDAEKKQEVDYEKTCWNLQSLFKSDDQWKKELKSFDKDTKELKNYIGKVTKSPTH
                     LSFALNIKEKLDIRLNKLSAYVKLKQDTNKNSYKYLDMSDSMSKSYGNYMGICSDLEL
                     EILKLSNKDYKKIISNKNINRKYGAYLRDIRRNKVHYLDDKSEDILSKVSSISSLPSQ
                     VYELFRNMDRKTNLTPAQYASELESVDRENRKKAYQDELITYNDNINTIAGLITGQVK
                     KNIFYSQERGYESSLEMYLESDNVDEKVYNNLIRTVNSNMDSLHKYIDLRKKVLKLDK
                     VHSYDMFVPMVKAVDNNINYEKAQSIVYSALSPLGKEYNDVVYKAFNEKWIDVYSHEN
                     KVSGGYCLSVYENHPYILLNYNNSLGSVSTLSHELGHAVYEYLSSKNQNYFNSNPSIF
                     THEVASTTNEALLYEMLIKEAKGNDEKAYYITQYLDLIKDTLYTQTMYAEFEKTIHEL
                     VEKGKSVNALVLNDVWGQLLKKYYGQSYELDQLSKVGWARIPHFYNSFYVYKYATGCS
                     AGVSFAQDILKNGSDNYMSFLKKGSSDYPINLLKSSGINLTNTKPIENTIKKFDSLVA
                     ELEKLL"
     gene            67149..68114
                     /gene="galU"
                     /locus_tag="PC700_000064"
     CDS             67149..68114
                     /gene="galU"
                     /locus_tag="PC700_000064"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDF2579399.1"
                     /translation="MQVKVKKAVIPAAGLGTRFLPATKAQPKEMLPIVDKPTLQYIIE
                     EAVASGIEEILIITGRNKKSIEDHFDKSVELELDLEKKGKKELLEVVQNISNMINIHY
                     IRQKEPKGLGDAIYCARHFIGDEPFAVMLGDDIVDNDVPCLKQLTDAYEEYRTTILGV
                     QKVNQEDTNKYGIIEAKNIEGRVYKVKDMVEKPESGKAPSNIAILGRYIITPEIFDIL
                     KDLPPGKGGEVQLTDALKILSKKEAMYAYNFEGKRYDVGDKLGFLEATVDFALKKEDL
                     KEDFIKYLKHVCSEFDKNNNVLYNNTDEKIDLIEVNEARELEIQK"
     gene            68614..70218
                     /gene="rho"
                     /locus_tag="PC700_000065"
     CDS             68614..70218
                     /gene="rho"
                     /locus_tag="PC700_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDF2579400.1"
                     /translation="MRRIFLILDDKVLEGLGSKTLVELREIAKELKIKSITTYKKNEL
                     IEIINSKSKNEEKVNEETKEKNEDIKEDIKNRKENIERESIEKEVEYKSPTKSYNKKE
                     IDTQNISQNQENRNQRNDYNKTTSVNDSNKSSNISNRMVRNNNKNYYMPKQVDESKIV
                     DEFNTSKEDEVVGVLEILPDGFGFLRGSNYLSTEGDVYVSPSQIRRFNMKTGDKIKGI
                     TRHPKSGEKFRALLYVQKINDENPDTAIQRNAFETLTPIFPEERLTLETNRNEIATRI
                     IDLISPIGKGQRGLIVAPPKAGKTVLLKSVANSIAKNHPNVELIVLLIDERPEEVTDM
                     KESIEGDVIYSTFDQVSSHHVKVAEMVLNRAQRLVEHGKDVVILLDSITRLARAYNLT
                     ISPTGRTLSGGIDPGALHGPKKFFGAARNIRQGGSLTILGTALVETGSRMDDVIFEEF
                     KGTGNMELHLDRKLAEKRIFPAIDIYKSGTRRDDLLLDDEEKTALWRLRREMSNNSVM
                     EITDKVIELIKRTKDNKEFVKSIKNL"
     gene            70371..70571
                     /gene="rpmE"
                     /locus_tag="PC700_000066"
     CDS             70371..70571
                     /gene="rpmE"
                     /locus_tag="PC700_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HDF2579401.1"
                     /translation="MQKEIQPKYNPVEVRCACGNTFVAGSTKDEIKVEICSECHPFYT
                     GKQKNIEKGGRIDKFKKRFKMD"
     gene            70716..71609
                     /locus_tag="PC700_000067"
     CDS             70716..71609
                     /locus_tag="PC700_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HDF2579402.1"
                     /translation="MAKQAVGGQAVIEGVMMQSKDKRAVAVRKSDGEIALKEDRIKSW
                     VRDKNIDKIPFVRGSFVMIDTMIQGIKSLNFSSEFFMEEAEEDKFDLFIKKIFKDKAN
                     DIIIIFSLVIAMLLSAGLFIFIPTLVGGAFSKVMPNDFMLNLIEGIIRIAILFAYIVL
                     ISRSKDIERVFQYHGAEHKSIYCYENDLELTVENARKFKRLHPRCGTNFLFIVMAVSI
                     ILFAFFGWPNPILRIFMRIICVPIVAGLSYEVIRVLGKYDNGFTKIIAYPGMMLQYFT
                     TKEPDDEQLEVALEALKAVVD"
     gene            71627..72475
                     /gene="prmC"
                     /locus_tag="PC700_000068"
     CDS             71627..72475
                     /gene="prmC"
                     /locus_tag="PC700_000068"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDF2579403.1"
                     /translation="MTIKDIIIKYSDKLKDISDTPRLDTELLLQKTLGVDRLYIHLNL
                     NKELTEEQKTKFMGFAEERLNGRPIAYIVENREFMGLDFFVKEGVLIPRPDTETLVEE
                     IIEICREKKDVSILDIGTGSGAITISLAKYIENSKIMSFDISETALEIAKKNAIINEV
                     GEKIKYINSDLFTAISDSNIKFDIIVSNPPYIKKQDIETLHKQVKDYEPYNALEGGED
                     GLDFYRRITEQGKKYLNKCGILAYEVGHNQAEDVINIMKSNGYKKIYTKKDIQGIDRV
                     VIGYNI"
     gene            72519..73583
                     /gene="prfA"
                     /locus_tag="PC700_000069"
     CDS             72519..73583
                     /gene="prfA"
                     /locus_tag="PC700_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDF2579404.1"
                     /translation="MLKKLEVLEDTYKDLSEKIGDPDVINDQKVWQKYIKEHADLEPI
                     VMKYREYKSVLDSIKESKEILQEESDEELRELAKMELAEMEEKVAPLEEEIKILLLPK
                     DPNDDKNVIVEIRGGAGGDEAALFAGDLFRMYSRYAERRRWKIELLSASDTGVGGYKE
                     VSFMIKGKGAYSRLKYESGVHRVQRIPSTESGGRIHTSTSTVAVLPEVEDVEVEINPN
                     DLRIDVFRSSGNGGQSVNTTDSAVRVTHIPTGEVVSCQDGKSQLKNKEQALKILKARL
                     YDKALAEQHKDIAAERKSQVGTGDRSERIRTYNFPQGRISDHRINLTLYKLDAFLDGD
                     IDEMIDALITVDQTEKMTAI"
     gene            73712..74392
                     /locus_tag="PC700_000070"
     CDS             73712..74392
                     /locus_tag="PC700_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HDF2579405.1"
                     /translation="MILKVTIIGLLAGVIGTGLGGVISAIFKREVDKYLSFFMGLSGG
                     IMLAVVVFDLMKESMDKMGIINTVIFTFVGALITMYIKTKLDVSGNMASGYLIFISIL
                     LHNLPEGLAIGSSFMSTESLGITLAIVIGLHNIPEGLAMALGLVCNKMKLSKVILFTV
                     IAGLPMGLGSFLGVYFGGVFTSLIGVFLATAGGTMMYVVLEEIFPHSKSVYCIIGFLL
                     GTMIVNYI"
     gene            74421..75461
                     /locus_tag="PC700_000071"
     CDS             74421..75461
                     /locus_tag="PC700_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDF2579406.1"
                     /translation="MKTIISNIDINNIDYEEIKIQAKLLREGKTVIFPTETVYGLGAN
                     ALDENAVKKIYEAKGRPSDNPLIVHIYEKEEVYDLAKDISDKAKLVIEKLWPGPITII
                     LNKKDIIPYKTSGGLDTVAIRMPSNVIARAIIKEAGIPIAAPSANISGRPSPTKAKHV
                     YEEMNGRVDGIVLGGDSNFGLESTVLDLTEETPMILRPGSITKEVLEELLGEVKLDPS
                     LSKKEDNQKAKAPGMKYKHYSPNADVYIISGKRENVATKINDMIKSNRDKGLKTGVMC
                     ISRNREFYDGEVIDLGNSLEEVASNLFDALIEMDKKGVDVIYSEEFPKTGVGQAIMNR
                     LLKSAGYKVIKA"
     gene            75478..75927
                     /locus_tag="PC700_000072"
     CDS             75478..75927
                     /locus_tag="PC700_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HDF2579407.1"
                     /translation="MNILIVCTGNTCRSPMAEAILRKAIKESGRSIEEYSISSAGIST
                     ANGMGASENSIEVLKEIGIDLSNHRSKVITKKLIDESDIILTMTKSHKEILVQAVPKC
                     KEKVYTFKGFANKNEEDISDPFGGNLDIYRSTMREIMYSVNEIVKKI"
     gene            75944..76396
                     /gene="rpiB"
                     /locus_tag="PC700_000073"
     CDS             75944..76396
                     /gene="rpiB"
                     /locus_tag="PC700_000073"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HDF2579408.1"
                     /translation="MKIGLGCDHGGYNLKKEIISYLESKGIECVDYGTNNATDSVDYP
                     VYGEIVANSVINKEVDYGILCCGTGIGISLAANKVPGIRCAVVSDVFSAKMSKAHNDA
                     NMLSLGERVLGKGLALEIVEAWINTDFEGDRHARRVNMIKSIEEKHNK"
     gene            76432..77061
                     /gene="upp"
                     /locus_tag="PC700_000074"
     CDS             76432..77061
                     /gene="upp"
                     /locus_tag="PC700_000074"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDF2579409.1"
                     /translation="MSKVVETNHPLIQHKLTLMRDKNTGSKDFRELLTEIAMLMGYEI
                     TKDIPLKDVEIETPIQKTSSKVVAGKKLAIIPILRAGLGMVDGLVSLMPAAKVGHVGL
                     YRDPETLKPVEYYCKLPQDIGERDIIVVDPMLATGGSAVAAIDLLKSKGAKSIKLANL
                     VAAPEGIAEVQKYHDDVDIYVASVDERLNEHGYIIPGLGDAGDRLFGTK"
     gene            77361..78176
                     /locus_tag="PC700_000075"
     CDS             77361..78176
                     /locus_tag="PC700_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HDF2579410.1"
                     /translation="MKNCIAVRGAGDLATAVIHKLHNSGFKVVALEIEEPLAIRRQIS
                     FCEAVYEKKVTVEGVECIFCNTEKEIEEVLSQHKVALIIDPKGEKLSKLNPIVLVDGI
                     LAKKNLGTTKDMAPLTIALGPGFCAGEDVDVVIETMRGHNLGRIITNGRAEKNTGVPG
                     AIKGFSKERVMHSQYDGIMENVCKIGDIVEKGDLLCYINNYEKKHEVRATISGVLRGL
                     LKDGAKVNKNLKILDIDPRKEEVKNAFTISDKARCIAGGVLEAILNNGIYPQN"
     gene            78402..78839
                     /locus_tag="PC700_000076"
     CDS             78402..78839
                     /locus_tag="PC700_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HDF2579411.1"
                     /translation="MRPSWDEYFMEIAEVVKKRSTCIRRQVGAVIVRDKQILTTGYNG
                     SPRNLEHCENIGCKRQELNIPSGERHELCRALHAEQNAIIQAAHNGISVDGATLYVTT
                     RPCVLCAKMCINAGILKIVYEGDYPDDMSTELLKEAGIELIKF"
     gene            79024..79251
                     /locus_tag="PC700_000077"
     CDS             79024..79251
                     /locus_tag="PC700_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HDF2579412.1"
                     /translation="MSKRNTHTEIAKLFSLISQIGLMIVISILGCTFLGKFLDSKLNT
                     TPVLTIIFLLLGVGGAFSGVYKTLIVYTRRK"
     gene            79256..79642
                     /locus_tag="PC700_000078"
     CDS             79256..79642
                     /locus_tag="PC700_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HDF2579413.1"
                     /translation="MDIKLLNQVRSVTKGIVIFDVVLIVLMLITSTLSKPVLLGLIFG
                     SIIAMLNFRLLAISLEKSVTFPAGKAQAYASAQYAIRLFIMAVVLIASATVPHLNIIG
                     TALGLLSTKFVILSKNFINKLKRKEA"
     gene            79645..80349
                     /gene="atpB"
                     /locus_tag="PC700_000079"
     CDS             79645..80349
                     /gene="atpB"
                     /locus_tag="PC700_000079"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDF2579414.1"
                     /translation="MNQAHYYLIGNFKISETILVSWGIMAFLAIASYFLTRNLKKVPT
                     SKVQIFLEFAVGGLAKMVTDVMGKNTVKRMPYMIPYIGSLFLFFACSNLSPLLGLRSP
                     TTDLDTTAAWAMITFFMIYFAGVKFKGLSYFKELAQPLAIMLPINIIGELARPISLSF
                     RPFGNILGGTIIMGLFYQLLGFISSLIPGLSIPIGEILIPVPLHLYFDLFAGLLQAYI
                     FIMLTMVFISSAGELD"
     gene            80418..80678
                     /gene="atpE"
                     /locus_tag="PC700_000080"
     CDS             80418..80678
                     /gene="atpE"
                     /locus_tag="PC700_000080"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HDF2579415.1"
                     /translation="METAIVAAASAIGAGIAVATGIGAGIGQGIAAAKAAEAVGNQPE
                     AKGDITSTLLLGVAIAESSAIYGLVISIILLFVNPFFKYLGM"
     gene            80801..81298
                     /gene="atpF"
                     /locus_tag="PC700_000081"
     CDS             80801..81298
                     /gene="atpF"
                     /locus_tag="PC700_000081"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDF2579416.1"
                     /translation="MEKALVGITWEFVFQIVNTFIIFLLLRKLLFKPVLNIIESREND
                     IKSDLAEGEKAKNEGLALKKEYESKINFAKDEGQEIIKQATIRAEQKSDDIVNTAKKD
                     ALDIKEKANKDIEQERQKVINEIKNDISNIALLAASKVIEKDLDKSKHEELIENFIKE
                     VGEAK"
     gene            81295..81840
                     /locus_tag="PC700_000082"
     CDS             81295..81840
                     /locus_tag="PC700_000082"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDF2579417.1"
                     /translation="MINVIANRYAEALFQLGEEENSTDVLFKELEKVVDMMTKVSKDF
                     YKVLKSPLVSKSEKKNLVEIIFSKEVSSNIKNFLKVLVDKDRISYLEDIELAYKELLN
                     KKNNVIDGVAISAIPMSETDIKELEVKLSNKYNKNVTIENVVDKTILGGVLVRIGNEQ
                     IDGTVKTRLDKMKEKLSEVIS"
     gene            81857..83359
                     /locus_tag="PC700_000083"
     CDS             81857..83359
                     /locus_tag="PC700_000083"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003405060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDF2579418.1"
                     /translation="MNLKPEEISSIIKQQIKNYENKVELTDTGSVLTVGDGIASVYGL
                     EKAMSGELLEFPGEIYGMALNLEEEVVGAVILGDDSEIKEGDIVKRTGRIVEVPVGEA
                     LIGRVVNSLGQPIDGKGPIAYTKTRPVESEAPGIIDRRSVYEPLQTGIKSIDSMIPIG
                     RGQRELIIGDRQTGKTSIVIDTILNQKGKDVICIYVAIGQKRSTIAQLVSSLEKGGAL
                     DYTIVVSATASESAPLQYIAPYAGAAMGEEFMYNGKHVLIVYDDLSKQAVAYREMSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDELGGGSMTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLQPELFYSGVRPAVDPGISVSRVGGSAQIKAMKKVAGTLKLAYSQYREL
                     AAFSQFGSDLDEDTKKRLAQGERIVEILKQGEHQPIKVENQVMIIYAVINNHLEDIPI
                     DNIARFESELYAFVDNNYPEISRKILGGEDFTHDLTDAINEFKEKFVVEV"
     gene            83387..84265
                     /gene="atpG"
                     /locus_tag="PC700_000084"
     CDS             83387..84265
                     /gene="atpG"
                     /locus_tag="PC700_000084"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HDF2579419.1"
                     /translation="MAGAGIKAIKTRMGTVESTKQITKAMELVASSKLRKAKEKAEDS
                     REYFNTLYNTVKDIAQTTRGIRNEFLKEREVKNKCYVVVAGDRGLAGGYNSNVLKESV
                     SHMKGKKETVITVGKKAHEFFSKRNYNIAKDINSVEACGYEDATEISQTIIDLYKKGE
                     VDEVYIAYTKFKSALSQEVEILKLLPLVFEKDTDEQGMAVKEEKQNRSKVQYIPSAEA
                     VLSYIVPQFISGTVYGGILESFASEQGARRTAMESATDNANEMLSNLELSYNRARQSA
                     VTQEITEIVGGVEALK"
     gene            84278..85672
                     /gene="atpD"
                     /locus_tag="PC700_000085"
     CDS             84278..85672
                     /gene="atpD"
                     /locus_tag="PC700_000085"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDF2579420.1"
                     /translation="MANVGKVVQIVGAVLDVKFDSEQSLPNLLNALVIKLGDKEIVAE
                     VAQHIGDDTVRCIAMSATDGLVRGMEVVDTGGPISVPVGDETLGRIFNVLGKPVDGKP
                     APKSAPKLPIHRPAPAYDELETTAEILETGIKVVDLLAPYLKGGKIGLFGGAGVGKTV
                     LIQELINNIAKQHGGISVFSGVGERTREGNDLYGEMSESGVINKTALVFGQMNEPPGA
                     RMRVALTGLTMAEHFRDEQGQDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLA
                     TEMGALQERITSTKKGSITSVQAVYVPADDLTDPAPATTFSHLDAKTVLSRQISSLGI
                     YPAVDPLESTSRILDPSIVGKEHYEVARGVQSILQRYKELQDIIAILGMDELSDEDKL
                     IVARARKIQRFLSQSFTVAEQFTGNPGQYVPVKETVRGFKEILEGKHDDLPESAFLFV
                     GTIEDAVRKAKGSM"
     gene            85675..85935
                     /gene="atpC"
                     /locus_tag="PC700_000086"
     CDS             85675..85935
                     /gene="atpC"
                     /locus_tag="PC700_000086"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HDF2579421.1"
                     /translation="MASEFSLKVVTPSNTFYDGDVEMIVVRTTKGDKGVLKGHIPFVS
                     GIPVGTLKIKKEGSFKEAKIAEGFINVTQDKTVILTESAEWI"
BASE COUNT        34744 a         9291 c        15830 g        26327 t
ORIGIN      
        1 attttttgtc aaaaaaaaca aaaaatacgt atttattttt tcatttcggg aaaactaaac
       61 acaggaatta tggactttat aaaaatttaa ttcttgagga gtgatataag tgaaaaaagt
      121 tgctttaatt ttagctataa taggtgcgct taactggggt gcaattggta ttctaggtac
      181 agacttaata ggtagtatct ttggtggaac atatgaaatg gtaagtagaa taatatactt
      241 cattgtaggt ttagctggat tatacttaat accaagttta atgtctgatg atagagaata
      301 atttatgctt ttatgttaaa tacctcattc taaatgttta gaatgaggta tttaatttta
      361 taaaaatatt aatttgtttc ttttttaatg gtaaaataga taagaattga agttttaaaa
      421 agaagtatta tgtaaaaaag gagtactatt aatatatgtt tattattaat gaattaaata
      481 agattgtaaa tgaagaagta atatcttttc atatgcctgg tcataaaaag ggacaggttt
      541 ataaaaagtt aggatataaa gatatactaa aaaatttgta taaaatggat acaactgaga
      601 tattaggaac agataattta cactttccgg aaggaattat aaaaaattca caaaaaaaag
      661 catcaagagt atttaaaagt gattatactt gctatttagt aaatggtagt agctgtggaa
      721 tacaatctgc aataatgtca gtatgtagtc ctaaaagtaa aataataaca aatagagact
      781 gtcatcagtc tgttataaat ggatgtattt taggagatat agatatagag tatgtgcctt
      841 gtgagataag tgaagagaca aatgtcttaa aagggatatc tataagtaat gtgattgata
      901 taatagataa aaacttggat gcaaaagcag tactgcttac ttatcctaca tattatggaa
      961 tgacatatga tttagaatac atatgtaatt atgcacatag caaaaagatg gtagtgatag
     1021 tagatgaagc acatggtgcg catttagggc ttagtgagag gcttcctaaa acagctcttg
     1081 agcaaggtgc agatatagtt gtacagagta cacataaaac tcttccagca tttacacaat
     1141 cgtcaatgat acatatacag ggagaccgag ttgatataga taaaatattg aacatgttaa
     1201 gaattacaga atcttcaagc ccttcatatt tattattatc ttctctggaa ttagcagtag
     1261 atatatatga aaataaagga aaagaattaa tggaagagct tttaaataat attagaattt
     1321 tcaaagataa agttgattca aatattaata tatatgacac aaatgataaa acaaaaatat
     1381 ttatatcatc taaaaacatg ggtttaacag gttatgaact agaaaatatt ttgagaaaca
     1441 aatataacat acaagtggag ttatctaatt attatggagt tcttttaata tgtacaatag
     1501 gaaatactgc tgaggatttc tctagtttag aaacagcatt aaacaatgtt gtaaaggaag
     1561 actttaaatt tgaaaagtta gaaaataaaa aataccctgt tgttattcca aagaaaatat
     1621 taacacctag agaagcattt tataaaacta aaaaaagtgt taaaatatat gacagcatag
     1681 gtaaaatttg tggtgaatgt atagttccat acccacctgg aataagtgta atatctcctg
     1741 gtgaagtaat aagtaaagaa ataatagact atctaatgtt ttgtcattct aaaggcatga
     1801 ttgtgagtgg attgaaagat gtagatttaa attttataca agtaatagat ttagaagaca
     1861 tttaaaaatt atgatataaa agtagataga agtagatagt ttaaaattgt gaagagaaat
     1921 aaatagactg cttatataga gctatctaat aaaagtacta aaaaagtaaa aagtgtttgt
     1981 atggaggata tgaaaatgaa aggtaagtta ataattatag agagtggttc agatgcaagt
     2041 ggaaaggcta ctcagacaga aatattatat gaaagattaa aaaaagaagg ctatatgatt
     2101 aaaaaggtag aatatccaaa ttatgaatct ccatcatctg ccttagtgaa aatgtaccta
     2161 aaaggagact ttggtaaaaa tgcatcagat gtagacccat atatagcatc aacttttttt
     2221 acagctgata ggtatgcatc atttaaaacc gattgggagg agttttacaa tgaaggtgga
     2281 ataattatcg cagatagata tacaacctct aatatggtac atcaggcctc taaaatggat
     2341 atttgtgata gagaagagta tttaaattgg ttagctgatt atgaatttaa tttatttaag
     2401 ataccacaac ctgattgtgt tatattttta gatgtgccta ttgaatttag taaaaagctt
     2461 atggagaata gaaaaaataa aattacaggg gaagaaaaaa aggatattca tgaaagtgac
     2521 atagagtatt tagagaaatc ttacaacaat gctttatata tagcagataa atatgattgg
     2581 aaaaaaataa attgtgtttt agataacaaa ttaagaagta ttgaaagtat acataatgaa
     2641 atttataaaa tagttcttga ttcaataaaa cctatggaga gataaatatg tattttgaaa
     2701 atattatagg gcaagatttt gcaaagaaat atttaacaaa ttctataaag aaaaataaac
     2761 ttaataatgc gtatatgttt gaaggaatgg atggaatagg aaaaaagaaa tttgcagatg
     2821 aattatcaaa actattactt gattatgaaa atctcgaaaa tagtccagac tatgtactca
     2881 taaaaccaga tggaaatagt ataaaaatag ctcaaatcag aaatttacaa tcagatatag
     2941 taataagacc tcataaagac tataaaatat atataatcaa taatgctgaa aaaatgactg
     3001 tggaagctca aaatgcactt ttaaagacgc tagaggaacc tccaaactat gctataataa
     3061 tcttagtcac aaacaacaag gaatctttat tagaaactat aaaatctaga tgtgatataa
     3121 taaagttctc acctatacca attgaagact taaaacgtta tttgataaat acaggtatag
     3181 aagaagaaag agctcaatta ttggctattt tttcgagagg aagtattgag aatgctctaa
     3241 atctatctca gtcttctgag ttttcaatga tgagagaaga tatacaacag tatattcaga
     3301 taatgttaga taaaaacata gtggagattt taaatatacc aaacaatatg gaaaaatata
     3361 gaggtaagat tatagcttta ttagatatga tgataaacta ttttcgagat atcatcctat
     3421 taaaagaaaa tgtaaacaag aacatgttaa taaatgtaga taagctagtt ttcattcaaa
     3481 atatgagtgg aaaaattagt tattctcaat tgtccaagat tattgatata atagaagatg
     3541 caaagagtaa aataaaaagc aattgcaatt tcaatataag catacaagtt atgtctttaa
     3601 atatatacga ggtgattaaa tgataaagat agtaggtata aggtttaaaa gtgctgggaa
     3661 aatatattat tttgaccctg ttgattttaa tatagagcaa gatatggatg tagttgtaga
     3721 aactgcaaga ggcttggagt atgggaaagt agtcgttggt ccaaaagata tggatgaaag
     3781 cgaattagtt tctcctttga agccaattat aagaatagct acagaagaag ataaaaaagt
     3841 atatctagaa aataaggaaa aggcaaaaga aacttttgag ttgtgccaac aaaaaataaa
     3901 agaacatgag ttaacaatgt ttttaataga ttgtgagtat acgtttgata ggaataagtt
     3961 gattttttac tttacagcag aaggaagaat agattttaga gaattggtaa aagacttagc
     4021 tgctatattt aaaactagaa tagaacttag acaaataggt gtcagagatg aagctaaatc
     4081 tataggaggt ctagggcctt gtggtagaaa actttgttgt tcatcttggt taggtgattt
     4141 tcaaccagtt tcaataaaaa tggcaaaaga ccaaagtcta tctctaaatc caacaaaaat
     4201 atctggaata tgtgggagat tattctgttg cttaaaatat gagcatgatg tatatagtga
     4261 agcaatagaa aaaatgcctg ttgtaggtgc attggtgcaa tcagatgatg gaaaaggaaa
     4321 agttgtagaa gtgaatcctt tattagaaca aattaaagtg gaatttcaag acaagacagt
     4381 aaagatatac acaagagaag atataaaaat acttagagag cctaaaaaat gtgatggctg
     4441 tggtggtaaa tgtaaggata atgatggatt agatgaagcc acactaagag agctaaagaa
     4501 attagaagat taaatttaag gtagggcttt tcctaccttt tttaaagcaa ttaatgctta
     4561 aagttattaa tatttgagga gaatttatat atgcaattaa aagattcaga aagaatagat
     4621 gatttacaat taaaaggcct taaaattata caagatacta atggtttttg ttttggtata
     4681 gatgcagttt tattagccaa ttttactaaa ataaaaaaag atgccaaagt agtagatttg
     4741 ggtactggaa ctggaattat acctattttg atagctggaa aaagtgaagc taaaaaaatt
     4801 attggcgtgg aaatacaaga agatgtatat gaaatggcaa ctcgttctat aaaattaaat
     4861 ggattagaag aaagagttga gattataaat gaagatataa aatacataga caaagtctta
     4921 gacataaatg gttaccatgt agtcacatca aatcctccat atatgcatat tgatggaata
     4981 aaaaatccaa atgataaaaa agcaatatca agacatgaag taaggtgcaa tttggaagat
     5041 gtaattagag cttcatccag attggttatg cctagaggaa aattcttcat gatacataga
     5101 ccaacaagat tggttgatat aataacttta gggagaaaat ataaattaga accaaaggtt
     5161 attcaatttg tgcatccaag acctcaaaaa gcaccgaact tagttttggt tcaatttgta
     5221 aaagatggaa gaccagagct taaaatattg gacccattgt atgtttatgg agaagatggg
     5281 aattatacaa aagaattaaa agcaatatat aataataaag atataggaga gttgtaaatg
     5341 agtgggaaat tgtacatatg tccaacacca ataggaaacc tagaagatat aacatataga
     5401 actttaagaa cattaaaaga agtagacttg attgcagcag aagatacaag acatagtata
     5461 aaacttttaa atcattttga aatctcaaaa ccactaacta gttatcatga acataataag
     5521 gattcaaagg gagattattt gataaataaa ctaattgatg gtgaaaatat agctctgata
     5581 agtgatgcag gtatgcctgg tatatctgac ccaggagaag agataataaa acaagctata
     5641 caaaataaca tagagataga agtattgcca ggtgcaacag cctttgtaac ggctttagtt
     5701 ggctctggta tggacacaca tagatttgtt tttgagggct ttttagatag agataaaaaa
     5761 gttaggaaag cacaattgga agaattaaag gaagaaagta gaactataat tttctatgaa
     5821 tcaccacata gattgaagga tacactaaaa gatatgctta aaattcttgg taataggaat
     5881 atttctataa atagagaact tacaaaaaaa tatcaagaaa taataagaga aaatatagaa
     5941 actaccataa aaatatttga ggaaaaagaa gtaagaggag agtttgtatt aatagtagag
     6001 ggttttcatg gtgagaagtc aaagaaagaa aattatgatg ggcttacaga tagggaatat
     6061 gttttaaaac taattgaaag tggtatgaac aaaaaagatg caataaaaat agtttgtaaa
     6121 gatagaaagt taaagaaaga tgtggtttat aaacaagttt tagatttata aaacctgaat
     6181 ctgaggtgat tttatggaaa tagggaaaat tataatagag agtttaaaga gggaagatac
     6241 acataaaata tttatagaat tagataataa gcatgaatta gaaaaaataa ttcctaaaat
     6301 aagctgtatg aagtctgtag gtgaatgtaa atatcatgta gtaaattgtt ttgaacattc
     6361 tataaatgca ttaaaagaat tagaaatagt attaaatgat aaggattttt ttccaataca
     6421 tctaagaaaa catgtgtcaa actatttaaa cacatatatt gaagaaggaa taaataagtt
     6481 acatgtatta aagttgggaa catttttaca tgatataggt aagcctgata gtatgacctt
     6541 agatgaaacc ggaagagttc atttcacaaa tcatgaaaaa ataggtgctc aaataattga
     6601 caatatggga ataaagttag agttatcaac agagacatat agacttataa gtaaatatgt
     6661 aagatatcat atgatacttt tgtctctata taagaaaaat gatttgagta ggaaagaatt
     6721 aattaatgta tttaacttag tagatgagga cactataggt gtaataatgc ttgggtatgc
     6781 agatatagtg tctacagtaa aattattggg caagagtgga gaagtcagtg tgttaaagac
     6841 atatatggaa tatattttaa caaattactt gtataagagt aattatacac atgtataaat
     6901 ttagttaata taattgagtt ttagatgatt aattgaagtt tgaaatatac ggatttcaaa
     6961 aacataaaag aagttgtctt tgttgtaaaa ttatgaaaga caactttttt tatatcaaaa
     7021 attgaaattt tattaaggat acttaatggt ctattaatta ataaaccatt agtatttaat
     7081 ttatattaca atattgattt tgcttgtatt ggcgttgata gtataacaga ggtcttagta
     7141 gaaccaattt tctttatagt atcaatcaca ttttctagct cgtacatatc ttttacaatt
     7201 acttttaaaa gtaagcaatc atcacctgtt atatggtgac attctacaat cctagggtcc
     7261 tttgcagctg actcaataaa ttctgtatat ccattgcttg gaagagaaat atgaataaat
     7321 gcctttataa ctctgcctaa tgaatctggg ttgacaatag ctttatatcc ttctataaca
     7381 ccagactctt ctaatctttt gactctttct gaaactgcag gagaagttaa accaactatt
     7441 tttcctaagt ccttcataga aattctccca tcatcctgta aaatttctat gattctgtaa
     7501 tctgtaacat ccataaataa acagtccccc ttaaataaaa ataattaata ctataaaact
     7561 tttttagaat ctatcatttg tgtttttaaa taccaaagat ttctagataa atcaacctta
     7621 taaatgtgtg gttttgaaag tcttttgtat tgttcccaga tagtacttaa ttcatattgt
     7681 acatgatttt ttatttcaag aacagctttt ttattatatt tgcatctgcc ttttatatac
     7741 aatggtttaa gaagctcttt aactttgtaa tttgtaaata cctttgtttt ccatgtataa
     7801 gtaggatgga agatttctaa aggtttagac tcatctataa cttcatcatg taacataatc
     7861 aaatctgcct ctgccttatt gtcttcgtta tatattctta taactttctt gtaaccagga
     7921 ttatttattt tttctggatt ctcagaaatc tttatcttag gttcaagaac tcctttttca
     7981 caagatgcag ctaatttata aactcctcca agagaaggtg aatcgaaaga tgtaatgagc
     8041 tttgtaccta ctccccatga atttatagta gctccttctg ctttaagtga tgttatggta
     8101 tattcgtcta ggtcattaga tgcagtgatg ctgatatctg aaaagccagc gttatctaat
     8161 tgttttttag cttctttaga taaataagct aaatctccag agtcaagtct tatacccata
     8221 ggtttgtatc ctttttcaga aatatttttg aatactttta ttgcattagg aactccactg
     8281 tttaatacat cataagtatc tacaagtagt aggcatttat ctggataaac atcagcatat
     8341 gcttggaatg cttcaagttc attatcaaat ttttgtaccc aactgtgagc ttgtgtacca
     8401 attataggta tatcaaacat tttccctgct agtacgtttg cagttccagc acaaccacca
     8461 actacagcag ccctagctcc atatatccct gcatctggac cttgtgcacg acgtaatcca
     8521 aattcaaata cagggtctcc ttgagctgca aagcaaactc tagaggcttt agtagcaata
     8581 agagactgaa aattaactat tgttaacaat gctgtttcaa taagttgagc ttgatataaa
     8641 ggtgccttaa cagtaattaa tggctcatta gggaacatga tagttccttc ctcaacagca
     8701 tatatatctc ctgtgaattt aaattctttt aaaaatttta aaaacttgtc agaaaataaa
     8761 tttaagtttt ttaagtactc taaatcttca tctgaaaaat gaagattatc tatgtattca
     8821 acaacctgat caataccaca aactatagta taccctccat cacaagcgtt ttttctaaaa
     8881 aacatatcaa atacaacaat gtcttcatga atattctttt caaaatagcc atttaacata
     8941 gtaagctgat ataaatcagt aagaagtgtt agatttctca ttatttctct ccttaattat
     9001 tattaaatat taaaaattgt aaaaactccc acacttatac taaaacataa tatagtacaa
     9061 ctttcaagta taaagtatta cctaactatc ataaaattat ttaaagagga ggatgattat
     9121 aataggaaaa atatggtttt atatggtatc gataggaata ataggaagct tgatttctaa
     9181 aaatatgggt gaattaaata aagttatatt aagtgaaaca tcaaaaggta tagagtttgc
     9241 aataagtcta gctggtatca tggcactctg gatggggata atgaatatcg caaaagaatc
     9301 aggtttaatt gaaaagatag gtcaaaaact aaatcctata atgagaagat tatttccttc
     9361 tattccaaag ggtcataaag caatgtctta tatagttatg aatattgctt taaatatgtt
     9421 gggtgctgga aatggtgcaa cagcatttgg tttaaaagca atgagtgagc ttcaaacttt
     9481 gaataacaag aaagatacag ccactaatga tatgattatg tttatggtaa tcaatatatc
     9541 atctatacaa ataattccat ttactataat caaattaagg atggatatgg gggctcaaaa
     9601 tccaagtgaa ataattttta cgactttatt tgcaaccata atatctacag cagtagcaat
     9661 aataacatgt aaattttttc aaagaagata tagataaagg ggatgtagat acatggagat
     9721 attttcaaac ttacttatac caataataat tctgtacata gtagtttatg gtaaatataa
     9781 aaaaatagat gtgtatgata gttttgtaaa aggagcaatt gatggattaa aagcagcatg
     9841 ggatatccta ccatatataa taggtatatt tttggcaata ggaatattca aaactggtaa
     9901 aggtcttgat atgttagaat ggctatttac accaatagca aatatgatga gtataccaaa
     9961 agaattaata ggtctaataa gtgtaaaacc tttatctgga agtggtgctt tagggatgta
    10021 cagtgaactt gcaaatagag ttggaatagg aagtttagta gaaaaaatgg gtgctacgat
    10081 tgtaggttct tctgagacta ttttttacac aatggcaata tactatggaa gtctgaagat
    10141 aaaaaatact agacatacat tatcatgtgc tatgattagc catgttgcag gtgtcatagc
    10201 agctgttttt atttgctatg taatatttgt ataatttgtt gacaatatgg gaaaaatttt
    10261 ataatatagt gattatatag aaacaaatag taatacaaat tacaagctat gaaaagaaga
    10321 gtaaataaag gttttttcta tcagagagct aggttaggtg aaagctagtg tcaaaagctt
    10381 tattgaagat ggtcttggag ctttttatct gagcaagtgt gctagggtaa aacggttgga
    10441 cacgttatag tctactaagg tatctataaa atgtttctac atttcatgtg aaacataact
    10501 attatagatg aattagggtg gtaccgtgaa taatctcgcc cctatgttaa ctcataggag
    10561 gcgatttttt attatttaaa tgaaagaatc atatgattac gaacgataaa aaattaggag
    10621 gtaaataaca tgagcaaacc gagtttttat gtaacaacac caatatacta tccaagtgga
    10681 ggattacaca taggacatac ttattcaaca gtagctgcag atacaatagc tagattcaag
    10741 cgtttctgtg gatatgatgt aaagttctta acaggaacag atgagcatgg tgaaaaaatt
    10801 caaaagaaag ccatagaaca aggaatgtca gagatagagt atcttgatgg aatgataaaa
    10861 gatataaagg ccttatggaa tactatggat atatcttatg atgattttat aagaactaca
    10921 gaaaaaagac atacagacat aatacaaaag atatttacta aactatatga gcaaggtgat
    10981 atatataaag gtgaatatga aggtagatac tgtactcctt gtgagagttt ctggacagaa
    11041 tctcaattac tagaaggaaa taaatgccct gactgtggaa gagaaacata tttagtaaaa
    11101 gaagaatctt atttctttag attatctaaa tatgaagaca gattaaaaga gttatttaaa
    11161 gatgatagtt tctgttttcc tgctgctaga aaaaatgaaa tggtggcaaa ctttttagac
    11221 aaaggattag aagatttaag cgtaactaga acgacttttg actggggtat aaaagttcct
    11281 tttgatgaaa aacatgtaat ttatgtgtgg gtagatgctt tatgtaacta tataacagca
    11341 ttaggttata tgactgataa tgatgaagaa tttaaaaaat attggcctgc caatgttcaa
    11401 atagtaggta aagaaatagt gagattccat acaataatat ggccagcgct tttaatggct
    11461 ttaggtctag aagttccaaa acaagtattt ggacatggat ggatactatt tgctgatgat
    11521 aaaatgagta aatcaaaagg taatgtggtt tatccagaac ctataataga aagatatgga
    11581 atagacacac ttaagtactt cttattaaga gagtttgcat ttgggcaaga tggaagttat
    11641 actcatagaa attttgtaac gagaataaat tatgatttag caaatgattt aggaaactta
    11701 ataagtagaa ctgtagctat ggttgaaaaa tacaataatg gtataatacc aacagcaaaa
    11761 gtttcaactg attttgatgc tgacttaaaa gaacaagctg tttcaactag agaaaacttt
    11821 gaagctgaaa tggataaaat gcaattccat gaagcattag agagtgtatg gaaattagtt
    11881 agaagaacta acaaatatat agatgaaact atgccttggg cactagctaa agatgaaacg
    11941 aagaaagatg aattagacac agttttatat aatttatgtg aatctataag aataatcgca
    12001 acactaataa atcctataat gaatgagact gctaataaaa tatatgaaca tataggaata
    12061 aaaggtcaag atgatataac tacatgggaa agtacaaaaa catttggtct tattggagaa
    12121 aatgtaaaag tatttaaagg tgaaccttta ttcccaagat tagatgtaga aaaagagata
    12181 gaagaattaa ctaaaatgtt ctctgggaaa cctccagtag aagaaaaacc tttagagcat
    12241 aaagaagaaa ttactattga tgatttagac aaaatagagc ttagagtagg gaaaataata
    12301 agctgtgaaa aacatccaaa agcaaataaa ttattagtat cacaagttaa gattggacca
    12361 gaaacaagac aaatagtatc aggaatagct gaatactata agccagagga tttagtagga
    12421 aaagaggtta cagtggtatg taacttaaaa ccagttaaat taagaggtgt agaatctcaa
    12481 ggtatgatat tagcagcagg tgatgatgga gacccatatg ttttaccatt tacacaaggg
    12541 gctaaagatg gatgcgaggt tcgttagtag gtaattaaat agtaaggaga ttaaagatgt
    12601 tatttgactc acatgcacat ttaaatgatg aaagttttga tgaagataga gatgaactta
    12661 ttggttcatt aaaagataaa ggtgtagatt tggttgtaaa tccaggagca gatatagaga
    12721 cgtcgataac agcaattgaa cttgcgaaaa aatacgactt tatatattca gcagttgggg
    12781 tacatccaca tgatgtatca aaactagatg atacagcaat agaaactcta agaaaacttg
    12841 caactgagaa tgaaaaagtt gtagctattg gagagattgg attagattat tattatgatt
    12901 attctccaag agaagaacaa aaagagtggt ttaagaagca aattgaatta gctaatgagc
    12961 taaaacttcc aataataata catgatagag atgcacatgg agatactttt gagataataa
    13021 agaatactaa aaatcctgaa ataggatgtg tacttcattg ttatagtgga aatgtagagt
    13081 tagctagaga atatgttaag atgggttgtt atatatcaat tccaggcacg gttactttta
    13141 agaataataa aaaaactaga gaagtagtaa gagaaatacc tctagaaaga ttattcatag
    13201 agactgattc accatatatg tcaccagagc cacatagagg aaagagaaac aatccttctc
    13261 aagtatcttt tgttgctgat aagatagccc aggaaaaagg gatatgttat gaagaagtat
    13321 gtagagttac aaaagaaaat gcaaagaaat tttttaatat aaaatagaca aattaaatct
    13381 tataatgata tatataataa gtcattataa gtataggatg agagtgcctc aaaatgagtt
    13441 ttttagttca tgaggcactc tttttttatg aaatcaaata tattttcatc atttcaacaa
    13501 tgaaaaagaa gtttatcata ttttgagaca gtttgttttt atttgtttaa ttcatatttt
    13561 aaaatatatt ttcttaacat tgttaaaaaa gaatttacaa gatattcatt agaattaact
    13621 caactctaat attcacttaa cttaagtggt ttattatatt attaaagaga agtaaacgaa
    13681 aggtagatta aggaggctaa attttgtata cagttgatag tactaagata ttgataaata
    13741 tgaacttaga gaaactagaa aagatatact caatgataaa agatacatca agtatttcta
    13801 taattaaaga acctaacttg gctacagtaa tggtaagagc taatgaaagt gtaaaaaata
    13861 caacatttaa tttaggagag atattagtta ctgaatgtag tgtaaaggta gatgaaagct
    13921 tgggttacgg aatagtttca gaaaacaata ataagaaagc catttactta gcagttatag
    13981 atgcagtttt acatagcaat aacagtaaat ttgatgagtt aaagaattac ataaataaaa
    14041 gtgtatatga agagaactta aggtatgaac aagagattat tgatgaattt agcttgatta
    14101 ataaaacgaa agttcaattc aatgctatgg attaaatagg aggagaatca tggtaaatta
    14161 tacacaagaa atatatagaa agatgctaga tagtgcttcg tttcctggga aattaaataa
    14221 tatagataat ataaatatag aaaataatac aaaactattg aatggagcaa ttggtatagc
    14281 aatcacttta ttagaccaag aagttacctt ctatatagaa aattatactc aaaaagatat
    14341 aaaaaatata agagcgctta cagtgtcaaa tcaaatagat tatagaaaat cagattatat
    14401 atttttagat attaattcag aagtggatat attacagatt aaaataggtt cttttgaata
    14461 tcctgatgaa agtgcaacta tagtacatca agtaaatgat ttatcaacgt actctcaaac
    14521 taagtatata aagcttgatt tgagtggtcc tggtataaaa tctaaaaata gtatatatat
    14581 aaatggaatt agagaagaat ttatagaaag attatctacc ataaataaag attatccaat
    14641 aggaatagat ttgattttag tagataaaaa aggagaaatt gcatttatac caaggtcatc
    14701 aaaattaagt tgggaggtat tgtagaaatg gcttatgtag cagttaaagg tggagaagaa
    14761 gccatattac aggctaaaaa tatacttgag ttttataggg taaaaggaga tagcttgcct
    14821 atagatgtaa agcagattga ggaacaaatg tcttatgctg tagacaaagt aatgtctgaa
    14881 ggaagtttat acaacaagga actatcagca ttggctataa aacaagctca aggtgatata
    14941 ttagaagcct ctttctatgt aagagcttta aggtcaacaa tgcctagaat tacatattca
    15001 gaacccataa atacagaaga aatgagaata aaaagaagaa tatcagctac ttttaaagat
    15061 ataccaggag gtcagtatct tggggcaact agagactatg aaaatagaat attaaatgta
    15121 gaattattag aagaagatga aaaaaagaat agagatttta gagatagttt tataaatgaa
    15181 ttagaaacag tagatgaaat ccctgaattt acaaaaatat ctaatatatt tagaaaagaa
    15241 aatttattac aagaagaagt ttgtgaaaac aaattggaag aagttattga tgatattaca
    15301 agaaatagtt taagatttcc agtttctagg tcagcaaagt tacaggcatt gactagaggt
    15361 gaaagtggag tagtgtcagg ttttgcttat gctattgtaa gaggttttgg tgatgaacat
    15421 ccatatataa gtgaacttag aactggtaat gtatttataa aagtaaaaca tcccatctat
    15481 gaagatgaag aaataataat aggtgatatg ctggttactg aatgtgaaat agtttcaaaa
    15541 gcatcacaaa ataagactga aaattctaaa actaattcgg aagataaaaa taagatgtct
    15601 ttaggatatg gtctttgtat ggggaataat gagaataagg ctatatctat ggccatacta
    15661 gacaaatctt tggagccaaa tagtgaaaaa ctagcacaag atgaagaatt tgtattgctt
    15721 catatagatg gaatagattc attaggattt atatctcact ttaagcttcc tcactatgta
    15781 gattttaaag cggatgtaga aagaataaaa tagtttgtgg aggtaagttt aggtgatata
    15841 taactatggt tttttagacg aaaacacaaa aaaagaaata agaagaaaga tattaaaagc
    15901 aatatcaata cctggttatc aagttccctt tggttcaaga gagttgccta tagctaaggg
    15961 atggggtact ggaggtttac aattaacact atcattaatt ggtaaaaatg atgtagtaaa
    16021 agtaattgac caaggaagtg atgattctac aaatgcttgt aatataagga gctttattgc
    16081 aagtgtaagt aatgtagaaa ccacaaaaga tacattagaa gctacattga ttcaaacgag
    16141 acatagaata ccagaagaaa aacttaaatc taatcaaata ctaatatttc aagtaccaat
    16201 accagaaacc cttagaatag tagaacctag tgaagtagaa acaagaagaa tgcattcaga
    16261 agaagattat agtaggatgt gggtatatct ttatgaagat atagttagat ttaatgatat
    16321 atctatagca gttgaatatc catgtaaagt aaatgataga tatttaatga atccatcacc
    16381 tatacctaga tttgatataa aaaaattaaa tatgtcagac aatttattct tgtttggtgc
    16441 aggtagagaa aaaagaattt atgctattcc tccatataca aaggtagaac ctttagagtt
    16501 tgaggattat aagtttgaag aggaaaagtt tgaaggaaaa tactgtagtt tatgtaacag
    16561 tacaaataca tttttagatg aagtatatga cagtgacact aatgaaaagt actatagttg
    16621 ctcagataca agttattgtg aaaaagttag attaaaaaat aacagtatag atgtgacaat
    16681 aggaggtact tggaatgagt aaaataaatg tctctaaaag attagaaaat atagacaagc
    16741 aagtattaat aggaagaaat atcaatctta tttatggaga tgggtgtgaa aagtgttttg
    16801 aaagcacagg atatgaaagc aataatatat gtaagtattg tggttctata gttgcatgta
    16861 ataatgtaaa tttagaactt tatgaaggag aagtccttgg aattgtaggt gaatctggtt
    16921 caggaaaaag cactttatta aaaatactgt ttttccaaga acatgcaaat agtggagaag
    16981 tatatatatc ccattacaat aaagacacaa atatactaga gctttcagac caaaaaaaga
    17041 gatacataaa gaatcatttt atgggtattg tgtatcaaaa tccccacctt ggtttaaatt
    17101 tagactttag tagtggtggt aatatagcag aaaaattatt gatggcaaat ttatataatg
    17161 ttgaaaaaat aagagataga ggcaaagaac ttttaataaa aactaatata cctgtagaaa
    17221 gaatagatca taaaccaaaa tactttagtg ggggaatgca acaacgagtt caaatagcaa
    17281 aagctctttc aaataaccct ccaatattgc tattagatga ggttacaaca ggtttagatg
    17341 tttctgtgca agcggaagta ttggatttaa taagagaaat ccaaagagaa cttaagattt
    17401 ctatgatagt tgtatcccat gactttgatg ttataaagat gcttgctgat aggacaattg
    17461 taatgaaaaa tggaagtgtg attgagtctg gtcttacaga ccaaataatg gaggacccac
    17521 aacacccata tactcagcaa cttataaatt cattgctata aatatcgttt gtaaataagc
    17581 aaaatggggg tttttaataa aatgaaagaa actatacttg aagtaaataa ttttagtaaa
    17641 gactttgagt tacatgcatt gaacaagact ataacggcat gtagtaatat aagttttgaa
    17701 gtttcaaaag gtgagttttt aggaataata ggtaaatctg gagcaggtaa gtctaccata
    17761 ttaaaaagta tctataaaac atatatacca acaactggag aaataatttt taattcagaa
    17821 atatatggta atgtagacct ggctaaaatt ggagaccgag aaatgataaa tcttagaaag
    17881 aaagaaatag gatatgtatc acagttttta aagacacttc cacgaataac tgcaattgaa
    17941 ctggtagtac attctttaat tgagagtggg tttgaaaaag attgttcgta tgatatggca
    18001 aaagatatac ttaaccaatt tgaaataaag gaaaatttat gggatgcgta tccaaataat
    18061 ttttctggtg gagaaaaatt aagattaaat ttagctcaag ctatggttaa aaagcctaga
    18121 cttttgttac ttgatgaacc aactgcatca cttgataatc aatctaaaat atatgtaaaa
    18181 gaaaaaatgt tggaacttaa aagtcaagga actacaatga taggaatttt tcatgatata
    18241 gagtttatgg aaactgttat agataagacc tttactatgg caaaagggac tataagtgaa
    18301 agtggggtag cctagatgat aaaactaatt cataatgcaa atattgtatt agaagatatg
    18361 attttagaaa atggtcattt aatcatagag ggagatacaa taaaaaaagt atcaaatgat
    18421 aggattgatt acgcatttga ctttaatgag atagatgaaa taatagatgc aaaaaactta
    18481 tatgtaatac ctggtattat agatattcac agtgatgcca ttgaaaagga aatagagcca
    18541 agaccaagta cattattacc atttaatatg gcattttatg aactagataa aaaactacct
    18601 gcaaatggaa taactacagt ttatcattct atatctttag gggatggagt aggagttaga
    18661 agtattgata actctttaaa gatgattaaa aatattgatt catataaaaa tatagatagt
    18721 aagagtataa atcataaagt tcatttaaga tatgaagtgt tgtattatga aggtttagaa
    18781 aaggttttag aactattaga tgaaaataaa atagactact tatcaataat ggaccattct
    18841 ccaggacaag gtcagtatac aaatccaaca ttttataaag agtatgctac taaagtgtgg
    18901 ggagttacag aaaattatgt agatacatgg ttagatgatt tagtaaatct gcatgataat
    18961 ttagattgga ataaaatagc taatattata ggtatagcca aaactaaaaa tataaatgtt
    19021 gcatcacatg atgatgatac attagaaaaa atggatttca taaaaaatat aggcataaat
    19081 gtatctgaat ttcctataac tcttgaagtt gcaaaacatt caaaaaaatt gggtgttcta
    19141 acttgtttag gtgcaccgaa tattgtaaga ggtaaatctc ataataacaa tttaaaagca
    19201 atggatgcta tattggaaga ttgttgtgac attgtgtgtt cggattatct tccatcagct
    19261 atgataaagt ctatgtgtat agttgcagaa agaataaata atttaaataa agcagtcagt
    19321 ttatttacct caaatcctgc taaagcagtt ggtatatatg atgaaagagg tagtataaaa
    19381 gaaaataaaa aagcagattt agttttagta gatattgata gtgaatatcc aaaggtttta
    19441 aatactattg taaatggaaa aacagtttat aaaagggagg tataaaaatt gctaacagag
    19501 ttacattgtc atacaagtat atcagataat aattttacta ctgaagaaat aataaaaaaa
    19561 gcagcagaaa agaatataaa tatcttagca ataacaaatc atgacacatt atctggattg
    19621 gatgaagctc ttgtgcttgg agagcattat ggaataacaa taataccagg tatagaaatt
    19681 tcagcctatg attataaaaa tagaaaaaga gttcatattc ttggatataa catagattta
    19741 aacagtcaag aaataaaaaa tttgtgcaat ccaatggtat gtgacagaca taaagcatct
    19801 gttaagatgg taaataaaat tatagattta ggatacaaaa tatctttgga agatgtaaag
    19861 aaatattcat caaagactgg aatatttaaa caacatataa tgaaagctct tatagataaa
    19921 ggatatacag ataatatata ttcaagtcta tataaagaat tgtttcatag aggaaacggc
    19981 aaggtatatg tatctttaaa atatgtggat tacagagatg caataaaagc tataaaaaat
    20041 tctggtggaa tttgtgtgtt agctcacccg ggtcaaatgg ataattttag cgctattgaa
    20101 gagatggtaa aagtaggatt agatggaata gaagtatatc atccatctca caacaaagaa
    20161 ctagaaaaag agtcgctttc ttatgctaag aagtataatc tagtggtcac aggaggctca
    20221 gattaccacg gattttattc tcaacataat tatgaacttg gaagcaagag cttaaataaa
    20281 gaagatttat ttaagtttag gttagcttta ggaggggtat tataaatgtc ttttttacaa
    20341 gtaaaaaatg taggtaaaag ttatggacaa gttaaggtgt taaaagacat atctattgat
    20401 attgaaaaag gagagtttat ttgtttacta ggtccaagtg gttgtggaaa aagtacactg
    20461 cttagaataa ttgcagggct tgaggataaa catggtggaa aaataataat aaatgataag
    20521 gatatgacta attcaccacc tgaaagtaga aattttggta tagtttttca atcatatgca
    20581 ttatttccaa atatgaatgt ttacaaaaac atagcctttg gactagaaaa taaaaacata
    20641 agtaaatcta atattgataa aaaagttaaa gaagttctag acgttgtaga attaagtgga
    20701 tatgagaaaa aatatccatc acaattatca ggtggacaac aacaaagggt tgcattagct
    20761 cgtgcgattg ctttagagcc agacttttta cttcttgatg aacctttatc tgcattagat
    20821 gcaaaagtaa gacttaaact tagggagcag attagaagtt tacatagaaa actaggcatt
    20881 acaactataa tggtaacgca tgaccaagaa gaagctcttt gtttagctga taagatggtt
    20941 gttatgaata gaggagaaat aatacaagtt ggaacgccaa aggaggtata taaaaatcca
    21001 gaaacacctt ttgtagctga ttttatagga acaataaatt ttatagatga tggtattaat
    21061 aagatagcta ttagaccaga agatataaaa gtagagtcta atagagattc taaagataag
    21121 gatataaaag tgggagaaat tttagatatc gaatttagag gatttaacta tagaattaca
    21181 gttgagtatc gttctaagca aatgaaatta gatgtagttt ccaaagttgc agaacaaatg
    21241 aaactgtgca taggttctaa aataaacttt aagataccta aagaaggaat tgtacaatat
    21301 aaatcagagg gatgtgctta atagctaggg gtgaataaat gataacatca aaaaataaaa
    21361 gaagaataag tgatagcaca atacaaaaat atttcttgat tttttcggta attgtactaa
    21421 tagtatttat attatttcca ttagtatcat tattaaaaaa tgcatttatg gatacaagtg
    21481 gaaattttat aggaatttca aactttagta aatatgttga aaatccaagt cttatagcat
    21541 catttaaaaa ttcaattttt gtatctacag tctcatcttt aatatcacta tgtttagcat
    21601 ttatatattc atacgcaata acaagaacaa atataaagtt taaagatttt tttaaaacta
    21661 tgggaatgtt tccacttttt gcaccaacta tgttatatgg tatttcactt atataccttt
    21721 ttggaaataa gggaatcttt acaaccatgg gtctggaagt accactatat ggtccattag
    21781 gaataattat ttctcagatt ctatttacat ttccacaagc ttttttaata ttatctgtag
    21841 cactttcgat ggctgattat agactttatg aagcagcaga tagcttgggt gctagtgaat
    21901 ttaagaaatt ttgcaaaata acaattcctg gtataaagta ctcattactt agttcattct
    21961 ttgtatcctt tatacttgca tttacagatt ttggagcagc taaggtagtc ggaggaaact
    22021 acaatgtact ggctactgac atatataaac aagtagttgg acaatttaac ataccaatgg
    22081 gagctactgt ttctatggta atgttaattc ctgtactaat agcatttaca ttagataaga
    22141 tggcaagtaa gaaacaaggt atgaccataa ctgcaaagtc tatgccatat aagattaagg
    22201 aaaataagtt tagagataga gtatttttaa tagtatgtag ttttataaca ttgctaattt
    22261 ttattttaat attcgtttca gttataggtt cagttataaa gttatggcca tataatttaa
    22321 gctttacaat ggaacactat acatttaaag gtattttggg aagtggaata aaaacatact
    22381 taagcagttt gaaaatatca actttaacag caatatttgg aactatattt gtatttttta
    22441 gtgcttatat aatagaaaag gtggataagt ttcctaaatt aagacaaaca gcttaccttt
    22501 tatcaatgat accaatggcc ttacctggct tagttttagg tatatcatat ataacttttt
    22561 ttaactcagc aagtaatcca ttaaattttt tatatggaag tacagcaata ttagttatag
    22621 taaatgtagt tcatttttat tcagtttgtt ttataacctc aaattcatct ttaaaaatgt
    22681 tggataaaga gtatgaatta gttgcaaaat ctataaatat tccattctat aaagtatttt
    22741 ttaatataac agttccgatg tctataacat caatacttga aatagtagta tattattttg
    22801 taaattcaat ggttactgtt tcagcattaa tatttttata tatacctcaa actcagactg
    22861 catcaatatc aatactaaag ttagatgaga taggatatat aggaccatca gctgctatgg
    22921 cagtattagt gctattaaca aatataatag taagactttt gtacgaattt gtaacaaaga
    22981 aattaaaaaa taacacacaa aaatggcagc aaaaagatgt tgcatagggg aggaaaaaat
    23041 gaaatttaaa aagctagttt ctttaggagt attatcaaca cttttattaa gtgtaataac
    23101 aggatgtagt actgattcat caggttcaga ttcaaagtct gatagtaaga caaaagggga
    23161 tttaacagtt tacacagcaa ttgaagaaga tagtatagag ccatatttag ctacatttaa
    23221 agagaaatat ccagatatta aattaaatat tgttagggca tcaacaggag atataacagc
    23281 aagattatta gcagaaaagg ataatcctca agcagatgtt gtatggggag ttgcagccac
    23341 aagcttattg gttgcagatg accaggggat gttagaacca tatgctccaa aaggttctga
    23401 agaaatagag tctgcattta aagatgataa agaagttcct tcttgggttg ggatagatgc
    23461 ttggatgaca ggtataacag taaacactaa agaattaagt gataagaaca tcccagttcc
    23521 tagttcttac gaagatttaa taaagccaga atataaaggt cttatatcta tgcctaatcc
    23581 atcttcttct ggtacagggt acttaacagt atcagccctt atacaaatta tgggggaaga
    23641 aaaagcatgg cagtatatgg ataagttgca tgagaatatt ggtgtataca ctcaatctgg
    23701 ttcagctcca gctacatcag cagcttcagg agaatatcct attggtatat cttttggata
    23761 tagaggtata aaattaaaag aggaaggcta tccagtagaa gtagtatttc caaaagaagg
    23821 ttctggatgg gatatagaag caaatgctct tgtgaaaaaa gataatataa aagaagcatc
    23881 aaaagtattt ttagattggg ctataagtaa agatgcaatg aatgaatact ctaagaacta
    23941 tgcagttact actatttcta caggaaatcc aataccagaa ggattcccta aaaagccatt
    24001 agagcaaatg atagataatg atttaaaatc agcagcaaaa aatagagaag atatactaaa
    24061 taaatggata tcaaaatatg atggtaagac agaaaaagag agttaagtaa aactatacaa
    24121 atggagtaaa aagaaaaaat ttttttaagt agattttatt ataataatat aagttgagaa
    24181 taatataggt caagaaaagt gaatattcta taaaaatgta tagagtaagt ataccattca
    24241 gtttgaatgg tatatttttt atttttagag attaatttag aaatagatat actacagttt
    24301 aaaatagtat attttaattt cattattaaa gtatagatgt tatatataaa gtaaataaga
    24361 tattaaataa ttataaacta aagtaaataa aatattgagc gattattaaa aagatatata
    24421 tatatatttt aatttgagca gtttgttata tattataaaa aaagagaaaa ataaaaggta
    24481 aattgtttct ggataactta aaattggaag taagaatatt taactagaga agaattattt
    24541 aagttcaggt tagctctagg agtagctgat tttataggag caatagattt tatggatgat
    24601 gatataaaca tgatagcaat tagaccagaa gatataaggg tagaatttaa tagagatttc
    24661 aaagaaatta tagacctcaa actcagaccg tatcgatata aaaactaaag ctagatgata
    24721 tagtgttgta tataggacca tcagctgtta tggaagtatt agtgctttta acaaatataa
    24781 tagtaaaact tttgtatgaa ttgataataa aaaaattaaa aaccaagaca taaaaatggc
    24841 aacaaaaaga agttgaataa gggaggtaaa aatgaaattt aagaagttag tctctttggg
    24901 aatattatca acacttttat tgagtgtaat aactggatgt agtaatgttt catcaggttc
    24961 aaacttaaag tctaataata agacaaaagg agagttaaca gtttatacag caattgagga
    25021 agatagtata gatacatact tagctacatt taaagagaaa tatccagata ttaaattgaa
    25081 tgtacttaga ttatcagtag gaggtataac agcaagatta ttagcagaaa aagataatcc
    25141 taaagcagat gtgttatggg gtgttgcagc tacaagtttg ataattttag atgacaaaga
    25201 aatgctagag ccatacgctc cgaaaggctt tgataaggta aatcctgcat ttaaagatga
    25261 taagaaagtt ccttcttggg ttggaataga tgcttggatg actggtataa cagtaaatac
    25321 taaagaatta aaagctaaaa acatatcagc tcctaattct tatgaagatt taataaagcc
    25381 agaatataaa gatcttatat ctatgcctag tccagcttct tcaggaacag gatatttaac
    25441 agtatcagaa ctcatacaaa ttatgggtga agaaaaagca tggcagtata tggataaatt
    25501 gcataaaaat attggtgtat acactcagtc tggttcagcg ccagctatat tagcagcttc
    25561 aggagaatat cccattggta tatcttttgg aaatagaggt ataaagttaa aaggtgaagg
    25621 ttatccagta gaagtagtat ttccaaaaga aggttctgga tgggagatag aatcaaatgc
    25681 acttgtgaaa aaagataata taaaagaagc atcaaaagta tttttagatt gggctataag
    25741 taaagatgca atgaatgagt attctaagaa ttttgcagtt actacaattg acacgggaaa
    25801 tccggtgcca gaaggatttc caaagaatcc attagaacag atgatagata atgatttaaa
    25861 atcagcagca agaaatagag aggctatact aaataaatgg atagaaaaat atgatggaaa
    25921 gacagaaaaa gagagctaag ccttacaata aaatttagta caaaaagcgc ctagaatata
    25981 agttttacat ctaggctctt ttgatatatt tatttaaaat gcatatttct atataaaaat
    26041 aaatagttta gtaccttgat taaattaaat aaaaacctta aaataaagag atgtaaagaa
    26101 aaactaaaaa cgaataaaag gtgaatttat atgataaaag aaataatagt agtagagggt
    26161 agagatgacg ttacagcagt taagagagca atagatgcag aacttataac tacaggtgga
    26221 tttggatttc caaaaggtgt aatgcagcgt ataaaagctg ctaatgaaag acgtggtgtt
    26281 ataatattta cagacccaga ttttgcaggt gaaaaaataa gaaaaaaaat agcatcagaa
    26341 gttccaggat gtaaacatgc atttctacca agagaggaag ctaaaaagga tggagatata
    26401 ggtattgaga atgctactcc tcaaagtata atagcagctc tgaataaagt tagaactgaa
    26461 aatacagaaa aaagagatga atttaagcaa gttgacttaa ttagaaatgg tcttataggt
    26521 aatgaagaag catcacttag aagagatgca cttggaaaaa tacttggaat tggatatgga
    26581 aatgcaaaac aatttttaaa tagattgaat aattatggag ttgaaagaga agaatttata
    26641 gatgcattaa aacaaatcta aacgtaatgt ttaatgtatg aaaaatatgt atataaaata
    26701 aattgtggaa ttacggttaa tttaagataa attatttata atatatacct tattttaaat
    26761 caatgtaaat acatataaat aagtataatt aataaaatta aatatcaaaa taagttaaaa
    26821 aggagtaaat aatggataga ctatcttcac ataatgcaac aaaggaagta gtacaaaaat
    26881 ataattttaa attttcaaag tctttaggtc aaaatttctt gatagatagt aatataattg
    26941 ataaaatctt atctggagct agaatcacaa gaggagataa tatcatagaa gtaggacctg
    27001 gtataggaac tcttactcgt gagatgggta aaatagcaga aaaagtagtg gctatagaaa
    27061 tcgatagaaa tttaattcct atattgaaag ataccttgtc agatttagat aatacagagg
    27121 ttgtaaatca agatatacta aaagtagaca tacaggaatt agtaaaagat aaattaaatg
    27181 gtggaccagt taagcttgta gcaaatcttc catattatat aactactcca atagttatga
    27241 aatttttaga ggaagacata cctgtaacag acatagtagt catggttcaa aaagaagttg
    27301 cagatagaat gaatgctatt ccgggtacta aagattatgg ggcattatct atagctgttc
    27361 aatactattg tgatacagag attgtggcaa aagctccaag acatatgttt ataccacaac
    27421 caaatgtaga ttccacagtt ataggtttac atgtaagaga taaaagaaaa tatgatgttc
    27481 ataatgaaga catatttttt aaaacagtaa aagcatcctt tggacaaaga agaaaaactc
    27541 ttcttaattc gttgggaggt cttggttttc ttaataaaga tgaaataaga gaaatattaa
    27601 aagaagctaa tatagacgaa aaaagaaggg gtgaaactct tagtatagaa gagttttcag
    27661 ttctttcaaa tataataaat acaaaagtat cttctaaata gaaggtactt tttttaattt
    27721 tgttaacata tattgtttag agaaactgtg ttaagggggg attgcagtgg gttctaaaag
    27781 aaagtttaaa agagactata taatactaga agctaaagac ataaacttta gatacaaaga
    27841 acgagtactg ccaaaagctt ttgcaaaagt tgaagtaaat gatgaaaaag caattattgc
    27901 attatatgta gaaaatctaa aatatgttaa agatgggtat aaagcagtag caataaaaga
    27961 tgaccatgga ttaatagact taggaaatat aattttaaat gaacaaggaa gaggtgaatt
    28021 tgttttagat ttagaagatt ctgatgatga gataaaaggt attgcattat tgtatgaaag
    28081 aaatatacct ttaattggat ttaagggaaa taagcttgaa aattatgagg acatattatt
    28141 tcaagaggta gaggaagaag aatatgaaga atatgaagaa tatgaagagt tggaagaaga
    28201 cgaagagata gaggataatg aggaaagtga tagagacgaa gatagtgaag aagaatatat
    28261 tatagaagaa tatgatgaag atgagtatga agactattgg gaagatgatg aagatattga
    28321 agaaggagag ttaaaagcaa gaacggaagt agaagattct ggtgatgagg aaatagaaga
    28381 gttcaataat tataaggaaa tagaaaatta taatagggtt aaagtacagg aagaatatat
    28441 agacatagaa tatgatgagg aagaagagga agaagaagaa gtttttgaag taaatagaga
    28501 agaagatagg tttgtttcct caaaacctac taaatcaaag aaagttcaaa aagatcagaa
    28561 taatattgaa aaaataaaaa gagctaatgg agtaagtcaa ggagagagtg gaaataaaca
    28621 aaccagtaca ttacttatgc caagacaaat aaaaaaaggt ttgaaattat ttaaagaagt
    28681 aaaacctttt gtaacagatt atatagataa gacaagatgg tggaaaatag agataaatcc
    28741 tacaacactg tgtggatata caatgcctta tttaggatat ttaaacgtat tgaattacac
    28801 tatgtatagt gatgcagttt tacattcata taaatacaga cactatttat ttggtgttca
    28861 gtatgatgaa tataataaga gaaaacatta tatttatgct atcccaggaa atagaaatga
    28921 acagccagac aatggaagca caggattttt aagatatcaa gcttgtgata atagaacaaa
    28981 tagtttagga tattggctat gttttataga ttctagacaa agaaatatag ttaggtaaaa
    29041 ataataaaaa tgaaaaaatt tagactcaat tcttatttta ctgaaaaaat taatgaaatg
    29101 tagtacaaaa ctttaaaaac tattgataat atttaaatat tacactacaa tatatcttga
    29161 aatacctatt atattatgga ggaaaattat gataaacgag caaagaattt taaatgagtt
    29221 tcttgaatta gtacagatag atagtttatc tttaaaagag gggaatgttg ctaaagtatt
    29281 agttaaaaaa ttagaagaaa taggttgttc agtagtaata gacaatgctg gtgaaaaagc
    29341 aaatggagaa actggtaata taatagctac attaaaagga aataaagaag gaaagaaaat
    29401 attatttagt tcacatatgg atacagtaac tccaggtata ggggtaaaac ctatagtgga
    29461 tgaagcaaat ggaataataa aaagtgatgg tactacaata ttaggttctg atgataaagc
    29521 aggtatagca gctatattag aaggcttaag atatataaat gaaaataaca tagaacatac
    29581 tgatatacaa gttgtattct ctatatgtga ggaatgtgga ttagttggag ctaaaaattt
    29641 agactatgga aaaatagatt ctgaatatgc atttatatta gatagtggtg gttctccagg
    29701 tgaaataata gtaaaagctc ctgcacaaga tgtaataaat gttaagatat taggaaagac
    29761 tgctcatgca ggtcttgaac ctgaagctgg aataagtgct ataatggtag ctgccagagc
    29821 aatagaaaat atgaatttac ttagaataga cgaagaaaca actgctaaca taggaattat
    29881 aaacggtgga acagctacaa atatagtaac aggagaagtt aatatagttg cagaagccag
    29941 aagtctaaaa gaaaataaat tagatgttca aactaaacac atggtagaga cttttgaaaa
    30001 agctgcaaaa gattttggag ctcaaattga gatagatgtc aatagagcat atacaccaat
    30061 agatgttagt gaggattctg aaataataaa acttgcaaag aaggcttttt caaacttagg
    30121 tatagaaggt catactgaat ctacaggagg aggtagcgat acaaatatat taagtaagaa
    30181 tggtatcgaa gctataactt taggaattgg tatgaaaaat gctcatacac taagtgaaca
    30241 tatagctata aaagacttat atgactctgc taaaatggta gtggagataa taaaggaagc
    30301 ataaatttaa ataatagtgt ataatatata tatcgcctag cttaagctag gtgatttttc
    30361 attttataga agattgataa tctctattga aagcaaagag tatacatttg catataagag
    30421 agtgtttaat ttatgttcag atgtaggttc ttaataatat ttagattgtt tcataaggga
    30481 ggaataaata tggaaacaag gtatgaagaa gcaaataaaa taacaataca atcaatactt
    30541 tggaatgtag tattaacaat aattaaggtt atagctggtg ttattggtaa ttctagcgct
    30601 atgattgcag atggattaca ttcagcatca gatattataa gttctatagg agtactaata
    30661 ggcaattatg tatcttctag acctggagat agagagcaca attatggtca tgagaaagca
    30721 gagacattag tatcatttgt gttatcaata ttgcttattt tcgtttctat aacaataggt
    30781 atagaggcaa ttaaatcttt atttaactta gatgcattaa gtgttccatc aatattacca
    30841 ttagtagtat cagttatttc aatcctgata aaagagtatc aatatagaat aacaataaaa
    30901 gttgctaaaa agataaattc accagcctta aaagctgatg cttggcatca taggtcagat
    30961 gctttatctt cagtagctgc ttttataggt ataggtggtt ctattttagg gtttaagcca
    31021 ctagacccaa ttgcgtcaat tgtagtggct atatttgtag ctaaagtagg aattagtata
    31081 cttataagtt ctgttaatga attgatggat gtttcagttg acgaagaaga gataaaagaa
    31141 ttaaaattta ttgtagcaga tacagaaggt gttaaaaatc ttggtgatat aaagactaga
    31201 aaacatggag ctatggcata tgtagatttg actatatgtg tggatgaaaa tttgacagtt
    31261 aagcaaggtc atgatattgc taccaagctt gaaaaacata taataaaaca tatggaattt
    31321 gtaaaaggca taacagttca cgttgaacca tgtactaatt gccaaggaaa taagtgtaat
    31381 aattaattaa aacttaaaaa aaagtgtaaa aagttatata attgttttat gattataaac
    31441 tctaggggtg gttaaatgag caagaaaaga aaactaaaga aaaaagctgc aatattaata
    31501 ggagtagttg ttttctttat aatatacttt atatccttta gaataggaat aaatatggaa
    31561 aaggataaaa gtgaagtgcc aaagaaggta gaaaaagaag aagaagataa taggtcttta
    31621 aggactcaaa taaaagaaaa gaaaaaggtt tatatatcag acaaaaatat agaaaacata
    31681 aaaattgaag aagatatgtg ggatgaaata aagttatttt tctcagattt taagaaagta
    31741 agaaatacta cttcttctta cgaagcagtt tatgaaggtt attctgatga tggagttaaa
    31801 ttttctactg accttaactt ttttagagta tatactgtaa ataaagaaga atattataaa
    31861 gtaccagtta gttctaagga tgcttttaag aaacttttag atgagagtat atatacttca
    31921 tttgaccttt taagtcatta taaagattgg aaagaggtta ctataactca tggaaaagaa
    31981 attaaaaaag ttcatagatg gaaatttgat gatttagcac ataagatgat ttcaaagagg
    32041 gttgtaggaa aagttcaacc tgagaaaagt aaggaaagaa gtgactataa ttttactata
    32101 aatataaaag gtgataattt tgatgtaaaa gttgatacaa tgggaaaaga ttatgtaaaa
    32161 gttacttcta agaaaaatgt atcttactat gaggttcata caagtttatt tgattacctt
    32221 aaaaatgaga tatttgaaat tggaaagaaa taagacttta agaaggctat caaattagat
    32281 atttgatagc ctaaattatt tttatatatt atttttcaac caacaagtct gcaaccttat
    32341 aaattggacc agcaccagca gttattacta aatcattatc cttaacattt tcacgtaagt
    32401 attttactat atcttcaaat tcttttatat agatggcatc tacattgttt tgatataatt
    32461 tctcaaccaa gtccttagaa tgtatatctc cagggtcttt ttccctagca gcatatatat
    32521 ctgttatgat aactttatca gcagcataga atgcctctga aaattcacct aaaagagatt
    32581 ttgttctagt ataagtatgt ggctggaaga tacaccataa agttgatttt tttaattttt
    32641 tggcagcagc taaagttgct tttaactcag ttggatgatg tgcataatca tctactacta
    32701 aagcattctt ataataccct ttagtttcaa atcttcttcc gacaccttta taaattttta
    32761 tattttttct tatagtctct aagtcaatac cagaaacaag agctgccatt atagcagaag
    32821 aagcattgta tatgttatga agaccatata cagaaagttc aaattctcct aaatctcttc
    32881 cttcatattc tattctaaat attccatgac cactattgtc aaagtgaata tcttttatta
    32941 cagcatcatt gtcagaatct ctaccgtatt ttataatagt agctttaaca tcatacaata
    33001 tgtcatcaat attttcatca tcgccattta ttatgaaata gccttctggg ggtaataatt
    33061 ttccaaattt gttaaaagaa gccttgattt catctattcc agagaaataa tcaagatggt
    33121 cttcttcaac atttaaaact atagatatct ttgggttaaa attaaggaaa ctatctacat
    33181 attcacatgc ttcagttata aagtggttgg aattacctat cttaacatta ccacctatca
    33241 tacttaagtt accaccaact agtatagttg ggtctaagtc tgcatattca aagattgtag
    33301 ataacataga tgttgtagaa gtttttccat gtgttccaga aactgctata gaatttttat
    33361 attccctcat gatttggcct aaaaaagtcg ctctgttcat taccaattta tttttttctt
    33421 tagcagccac aagttcttca ttgtctggat gaactgctgc tgtataaaca accatattta
    33481 catcatctga tatattatct tttttctgac caatataaat agttgcccct tggtccctaa
    33541 gtttgtctaa taggtatgat tcgtttgaat ccgaacctga aacttgataa cctttattga
    33601 tacatatttc agctaatgca ctcatgctga taccgccaat tcctataaag tgtatattca
    33661 taataaaacc acctttcttt ttaattaagt cctacaatat gaactattta tgtggtataa
    33721 taatataatg ttcaatattt tataattaat atgcatataa tattatatgt attttatttt
    33781 tgaatataat aaaacataag taatattata ccacaataag aattgttatg cattagttag
    33841 tttaacgtta tataaaggta cttgtaggag gaaataataa tgaaatttaa aagaacagag
    33901 agaattggag caatagttaa aatactgtct gataatccaa ataaaatata tacattaagt
    33961 tattttacaa atcaatttaa tgcagcaaaa tcaacaataa gtgaagattt attagttgta
    34021 aaaaatgtat ttgagaagct acatttaggt aaagttataa ctatctcagg agcagcagga
    34081 ggagtaaaat atatacctaa aacttctata gcagaaaatg cagagttctt aatggaatta
    34141 tgtgagagaa tatgtgacaa gtctagaata ctatctggtg gttttttata cttgattgac
    34201 ctaatttatg accctacaat agctgctaag ataggaaaga tattcgcatc aaatattgag
    34261 tatgtagatg cagattatgt tgtgacaatg gaaacaaaag gtatacctat ggcacttatg
    34321 actgcaaaag ctatgaattt acctctagtt ataataagaa aagatataaa ggtatcagaa
    34381 ggtcctacac ttagcatgac atatgtgagt ggaaacagct ctaaagttga aagtatgagt
    34441 ttgccaagaa aagctcttaa accagatagt aaagttataa ttatagatga tttcatgagg
    34501 ggtggaggta ctataaaagg aatggtagac cttatgaatg aatttggtgc agaggttata
    34561 ggtactggag tattcatatc tacaacgaat ccttcagaaa aaatggtaaa agattatatt
    34621 tcattgattc aattggatgt taatggagat aaaatagtag tagaaccaaa cttaaagact
    34681 tttaaagatg aatatagaaa tgaagattta gacgaagaag aagattcaga atttgaattt
    34741 gagatagatg aagattaata aaaaaaatat ttagaaaaat atgtaaaaat aaaaagagga
    34801 tatccctagt tgttcataga attatttaaa aaacagttta tattaattta ttccagcgat
    34861 aataatttta atataaatta atttctgtca aaatcaaagg gggatatttt ggtatgaaga
    34921 taactgacgt aagagtaaga aaattaacag aagaaggtaa gatgaaatgt atagtttcaa
    34981 taactttcga taatttgttt gtagtgcatg atataaaagt tatagaaggc cataatgggc
    35041 tatttatagc aatgcctagc agaaaagtag gcgaaggaaa tttcagagat atagctcatc
    35101 caataaatgc agaaatgaga caagttttag aagatgcagt tttacaagca tatcatgaag
    35161 cattagttca atgggaagta gctgctgaat aataaatata taaaatggag agagcccgaa
    35221 catttaggct ctttttaaat gtagaatttc tattaaaata tgtacttttt agtaaaaaag
    35281 tgctatatta ttagtgataa tgtatatgtt gtatacaaat tgttagataa atgtattata
    35341 atgtatattg attaagagaa taatacgaat aaaattaaat tagattgaat ggagtgtatc
    35401 aatgaacttt aaagctataa tccttgctgc tggcaaagga acaagaatga agtctaaata
    35461 cccaaaagtt atacataaag tatgtggaaa agaaatggtt aaccatatta tagatgtttc
    35521 aaaaaaatca ggggtaaaag atactgtcgt gattttagga catgaagctg aggtagttaa
    35581 agaaaagctt gcagaagaaa ttataatagc catgcaaaca gaacaacttg ggacaggaca
    35641 tgctgtaaag atggctaaag aatatattaa tgatgaagat acaatagttg tactttgtgg
    35701 agatactcca cttataaaag aagagacact gaagagatta tttgaatatc atatagaaaa
    35761 taaatatcat gctacagttc ttacaacaag agttggaaat ccaacaggtt atggtagaat
    35821 aataagagat aagaagggtg accttcttaa aatagtggaa caaaaagatg caaattcaga
    35881 agaaaagatg ataagtgaga taaactctgg aatttattgt tttaatggaa agagtcttag
    35941 agaagcacta gatttgctaa ataataacaa ttcacaaggt gaatattatt taactgatac
    36001 tgctaaaata atgagagata aaggattaaa agttggtgca tttgcaggtt caactattga
    36061 agaacttatg ggcgttaatt caagagtaga attatcaaag gcagaagaaa ttatgagacg
    36121 aagaataaat gaatctcata tggtaaatgg agtaactata atagacacta actctaccta
    36181 tatagaatct gatgttatga taggcaatga tactataata tatccaggtg ttatgttaca
    36241 agggaaaaca aggataggtt cagattgtat aattggaatg aactcatcta taacaaattc
    36301 agaaataggt gatggtacag aaataaaaaa ttcaactata attgatagta aggttggaga
    36361 aaattctaat gttgggccat atgcatactt aagaccaaag agtgacttag gaaataatgt
    36421 aaaaataggc gattttgtag aagtaaaaaa tgctattata gaagatggat caaaagcatc
    36481 tcacctttct tatataggag atgcacatgt cggtaaaaat gtaaatattg gatgtggagt
    36541 agtatttgta aactatgatg gtaaaaataa atttaaatca gtagtaaaag acaatgcgtt
    36601 tataggttca aattctaatt tagttgcacc agtagttgta gaagaaaaag gatatatagc
    36661 aacaggttca acaataacac atgatgtccc agatggtgct ttggcaatag caagagaaag
    36721 acaagttatc aaagaaggat gggtagaaaa aaagaatcaa aaagacgacc aaagtaagta
    36781 attatgtagt ttattatttt ggctaaaaaa tttagctcga aattattata aattatattc
    36841 attagtattt taaaattttc aggagggtta cataaatgaa tactagcgga agcgagatta
    36901 aaattattgc aggtaattca tcgaaagagt tagcacaaaa aatagctgac tatataggtg
    36961 tatcagtact agattgtgaa gtaggtacat ttagtgatgg tgaaatatgt gtaaacatga
    37021 atgagacagt aagaggttgt gatgtgtttg tagtgcaatc tactaacagc ccagtaaatg
    37081 ataacttaat ggagttatta atcttaattg atgcgttaaa gagagcatca gcaggtagaa
    37141 taacagcagt cattccttac tatggatatg caagacaaga cagaaaggct aaggcaagag
    37201 atccaatcac tgctaaatta gttgcaaatt taataactgc tgctggagca gacagagtat
    37261 taacaatgga tttacatgca gctcaaatac aaggatattt tgatatacca ttagaccact
    37321 tactaggtgg aacaatatta gcaaattatt ttaatgaaaa gaaaatagaa gatttggtag
    37381 tagtatctcc tgacttagga agtgttacta gatcaagaaa atttgctaat actttaaatg
    37441 gagaagttcc aatagctata atagataaaa gaagaccgaa agctaatgta tgcgaagtta
    37501 tgaacttaat aggtgatgtt aaaggtaaaa atgttatact attagacgat atgatagata
    37561 cagcaggaac aatagttaat gctgcaaatg cacttaaaga gtttggagca aaagatgttt
    37621 atgcttgttg tactcatggt gtgttatcag gtccagctat tgaaagaatt gctaattcag
    37681 aaataagtga gttaatagtt cttgatacta tacaacttcc tgaagagaaa agaatagata
    37741 agataaagat aaaaacagta gctcctttat ttggagatgc tataagaatg atattctcta
    37801 atgaatcagt aagtaaatta ttctaattta ttgtatttat gagagttgtt taaaaggata
    37861 taaacctttt gagcaactct tttttgattt tatacttatg gaatgttatt tttttcttgt
    37921 tagaaaggta tatattattg taaaaagaga ttatagttta aaattatgta tatttaagta
    37981 aaataaactt aataaagggc atattattca aaatattatg aatataaaaa atattacaaa
    38041 aaagtattga aaaaattcag attatgtagt attatgtaaa tataaataaa gaaagaggtg
    38101 tttccaatgg gaaggttaga agatgaaccc ctaataatta aatgattcag tcacaatcta
    38161 aagtgaatat aaaaatctat tgaaagttta tttataagaa ttgaaaatag tatctattct
    38221 atctttagtg tttgtgagag ggttctggat tatataagat tactgtaaag agtaattttg
    38281 cacaataagc atagctgtaa atgaagagtt tcgtttatat aatgattgtg gctgaatcat
    38341 aactataata gatagaatgt ttataataga tgattttata aaatactata ttgtaatagc
    38401 ttttagcttt caaataaata ttagcaaagg atggaaatga caatgataaa actaaatgat
    38461 aatttataaa tatctcctta cgatatataa taattgtagg gagatgtttc ttttttttgt
    38521 agagtataat ttttctagaa attctgtttc aatatgtaaa aagttggtat atataaatag
    38581 tataaacagt gttgctaaaa tatggtagat attatataat ataagtggat atgtatatac
    38641 attatgagtt atataatttt gaatttaata aaatagaaac gttgatttat gttctgtaat
    38701 gtgggcacct tagagcatat tgagttagtg gtgcaaccgg ctatgaaatt gtatttattt
    38761 atagatacta tcattttcat agcttttttt atttaaaaaa tggttatgtg ttagaaataa
    38821 atttggttag tacgtttaaa taattttact tagattttta accagatata ttataagtaa
    38881 gtattaatta aaaaatttaa gggggaataa aaatgaagtt aaaaaagaat aaaaaaggtt
    38941 tcactttagt ggaattattg gtagtaattg caattatagg tatattagca gtagtggcag
    39001 ttccagcttt atttagtaat ataaacaagg ctaaggtagc aagtgttgag tctgattata
    39061 gttcagttaa gagtgcagca ttatcttatt attcagatac taataagata ccagttacac
    39121 cagatggtca aactggttta agtgttttag agacttatat ggagtctctg cctgataaag
    39181 ctgatatagg tggaaaatat aaattgatta aagttggtaa taaattggta ttacagatag
    39241 gtacaaatga tgaaggagtt acattaacag aagcacaatc agcaaaatta ttgagtgata
    39301 taggtgaaaa taaaatatat acaagtgtta cagcagataa tttgggaaat ccattaacaa
    39361 gtaatacaaa agtagataat aaagttctat atatagtact tatagataat actgtgatgg
    39421 actcaacaaa atagttaaaa ctatattttt ataaaaatat tgtaaattta atgaaaaata
    39481 taatataaaa gacaggaaga aaatcttcct gtttttttat actattttct ttaatgttca
    39541 attatgaatg atgatataat tgagatagta aaggagtgaa acctgtggct aaaaaagtta
    39601 gaataggaga caaactggtc gaaaaaggat atataacaga agaccagctt aaatgggcac
    39661 tatcagaaca aaaaaacagt ggaaaaagac taggagaatt tctagtacaa gaagggctta
    39721 tagatagtaa cttactaata agtgtattaa aagaactctt ggatatagaa agtatatttt
    39781 tagaagggac agaaatagac actttagcta caaagatggt tccagaaaat atatgcaaaa
    39841 gatatactgt atttccattt aaaattgatg gaaataaaat atgtctagcc atgtcagacc
    39901 cacaagacag agaagcagtt caagatgtaa ggcgtatgtc tggtaaagac gtagagatat
    39961 ttatatcaag tacagaagat ataaacaaag caataggtca tgcttatgca cattcagaaa
    40021 taaataaggc aatgactgag tacaacaaaa atagaactgg tggagtaaga gaaacagtaa
    40081 tacttgaaga agatgtaaat gctgctccaa tagtacgatt agtaaataac atactagaaa
    40141 atgctgttag aatggaagca agtgatatac atatagaaca aagtgaaaat tacatgcgtg
    40201 ttaggtttag gatagatgga atgttaagag aatatatgag gatgaactca gcaccatata
    40261 aggcagtaat cagccgtatc aagataatgt cagatataaa tatatctgaa aagagaatac
    40321 cacaggatgg tagaatctat ttaaaagtag ataataaacc catagatttt agggtttcta
    40381 caatgccaac taacagggat gaaaagatag ctatgagggt tcttgataag agtaatttta
    40441 tggttagtaa agaagtacta ggaatagacg aacatggctc taagatatat gatgaattga
    40501 taaacactcc ttatggtctt atattggttg ttggtcctac tggaagtggg aaaaccacta
    40561 cattgtattc aatgttaaat caactaaata cagaaaatag aaatctatta acaatagaag
    40621 accctataga gtatgaatta cctggtgtaa atcaatctca aataaatgaa aaggcagggc
    40681 ttactttcgc cagcggactt agagctttta tgcgtcaaga cccagatata ataatggttg
    40741 gagagataag agatactgag acagcagaaa tagcaattag agcttctctt acaggacatt
    40801 tagttttaag tacattacat gcaaatacag cagttggagc aatttcacgt cttttagata
    40861 tggatgtgga aagtttttta ataacttcat ctgttttagg tgtaatatct caaagactta
    40921 cacgaaaaat atgtgagcat tgtaaggttt catatgaagc agatattgga gaaaaaaagg
    40981 ctcttggtat tgatgtaaat gaatcagtaa ctatttatag aggaaaaggt tgtgaaaggt
    41041 gtaataatac tggatataaa ggacgtctag gtatatttga aatgcttgaa attactccag
    41101 aaataaaaga gttaatagat tcatctgcaa atcaaagaga aattttaaag atggctagaa
    41161 aacaaggcat ggttagctta aaagaggata tagttaaaaa ggttttaaat ggaaagacaa
    41221 cagtagaaga aatgattaga ataattttga tgactgatta aaggatgata ttatgaaaag
    41281 ttttaaatac acagtaataa aagaggacgg aaaaaagcaa actgatgtga ttgaagccaa
    41341 cgattttaat gaagctagaa atcttcttag aaagagaaat cttagagtta ttgaaatcaa
    41401 agaaagcaaa aataaaaata taagattatc tagtagaaag agaaaaaaag acttgggagc
    41461 tgaccagata agccactttt gtagacagtt tgctattata gtatcttctg gtataaatag
    41521 tatttcagga ttagaaacat tagctagaag gtcaactaac attacattaa gagaagaaat
    41581 taatcgtata gtggcagaaa taaaagtagg ttctactatt gcagattcaa tgctttcatc
    41641 aaaatcaaag tttccaaagt tacttggtgc tatggtagct acaggtgaag ctacaggtaa
    41701 attagatgaa gtattaaaaa gtatggcttc attttatgca agtgaacaca gggttaaaca
    41761 aaagcttaga aatgctgcaa catatccatt aatagttttg atttcttcat ttataatgat
    41821 ttttatattt acaacattta tggtgcctaa gatgattaat tcaattacaa cagtaggagc
    41881 aactcttcca cttataacaa gaattgttat ggggtttggt atgtttatga agaagttttg
    41941 gataatagta cttttagtta ttatattatt tatctatcag tttaaaaaat atttaaaaac
    42001 gtcagttgga agggctcata aagatagagt tataaacaag atacctgttt taggaaaagg
    42061 aataaattgt atggtagcta cacgattttc aagagcattg tatctatttg tatcaacagg
    42121 atacccatta gttcaagggc ttgattatat tatagatagt gtaaataaca ccatggcaga
    42181 gaagatttta gcatcagcta aagatggaat aacaagagga gaaggcttag cagaaaattt
    42241 agagagatat acgtattttg actctgtgtt ggtacagatg atagctatag gggagcagac
    42301 aggggaatta gagaatataa gtagacagat ggcagaattt tatgagtatg aatccgagat
    42361 ttatttaaat aggatggcct caatgataga gcctgtttta ataattgcag ttggtattat
    42421 agttgcgata ctggttgtat caatatttat gccaatgctt agtatatatg atgccatgta
    42481 ggagggaaag gacaaatgaa taaggtttat gtatcatatt atggcgattc tatttccata
    42541 gttgagggca gatataaaca cgataaggat aagttttatg taaaaaattt taatgtgttt
    42601 tcaatagagg atattgcaca agatttttct aaggaaaagt atgctctatt gagatacgca
    42661 ttagaaaatg ttaagttaaa atcaagacat gtagtttttt gtttaaatac aagagatgta
    42721 attttaaaac cccaaaaact tcctaatatg gacaaaaagg atttagatgg atttatgaag
    42781 ctagagatgg acgaaatgat ggctttggag tcagatgaat atgtattttc gtatgaagta
    42841 tctagtgaaa cacatgataa ttcagatggc gatgaatcaa gcttaaatct tatcattgga
    42901 gctatacaaa aggaagaggt aaatgcaatt gtagagataa tgcatgaatt tgatttagta
    42961 ttagatagaa tagatacatt atcaacagct tacttaagaa tgcttaaaaa tttagactac
    43021 aatgatataa tggtaaccaa tataagtgac aatagtacaa ttataaatat atataagaaa
    43081 gatactttat ttattagtga taatatacct ataaagataa ctgctaaaga tatggacatg
    43141 caattattaa atgtagtaga agaatcaaaa ggtcttatga attactattc gtcaagaaac
    43201 tttggtaagg ttacggatac tatattaata ttaggaaaaa gatattccaa taaattaatt
    43261 tatgaaactt ttagctctgc attttcaagc tatgtttctc aaggccttac tgagataatg
    43321 gatatatctg atatgataac tggagatata gatgaagatg atataaattc aattgtagag
    43381 cttttaggaa gtatgataga acctaaaggg aaaagagatt ttgaaaatat aaacttttta
    43441 ccaatggata tacttagaaa acagataaaa gctaaacaaa tgaaaaacat actgaaaatt
    43501 gctccaatag ttattttgat tcttgctata ccttatttat gtttaatttt ttcaaataat
    43561 ttagcaaata aggaacttga aaatgttaat ggtgaaataa gaaatattga atctgcatat
    43621 tatcagattg gaaatataga aaaaatgata cagagtaaaa gcgaagaaat aaaggtatat
    43681 gatatgttaa ttgggaaaaa ggtaaagtgg gaaaatttat tggatgatat agataaaaat
    43741 actccaagta aagttgaact aacaagttta tcaacaactt atcaaaatct tggaactaat
    43801 aataaagctg ataattcaaa aagtaataag actacaacag ataaatcaag taacaatgaa
    43861 acaaaatcaa gtacgaaaag tgatagtaat ggagataaaa cagataatga tttaaatgaa
    43921 gataagacta caaatgtgtc agaagcatta actggaaata gtgagactaa tagtaataac
    43981 acggataaag atgataaaac tccattgtat gataagatac caaatgttat aaatatagaa
    44041 ggaaatgcta aagattcctc ttatgtaggt caatttttat ataaattaaa gggattatac
    44101 tatttcaagg atgttaaatt acatagcata aaaagtaatg cagaagattc taactataca
    44161 tttagtatga cattatacct gaaagaaggt gtattaacta atgaataaag atatatttaa
    44221 aaaagatata aaagatatat ttaaaatgga agttagtcta aaagggattc ttataaaggt
    44281 tataatttta ttagctattt tatcatatgg ttattttgga ctttaccctc aatatcagaa
    44341 atatattgct acaaaaaatt ctgtaaaaca ggctactgaa aaattaaata tatataaaga
    44401 gaaaactgca cttatgccaa aatatgaaaa acaacttgaa gaagttgaaa gtgaattaaa
    44461 agaaaaaagc aaaaatcttt tatatgatat gcaagatggt ctttttttaa taggtttaga
    44521 taaaaaaatg aaggcttata atattgactt aactagttat gacgttggaa atgttaaaga
    44581 atacgaacat ttttatggta tatcaacaac aataagtgtt agaggtgatt atagaaatgt
    44641 caaaaaatta atggcttatt tagaagaaca aaaaaatgtt actcaaatac tagattatac
    44701 aatgacttca tatatagaac cagaaaaaga agaggaagaa acaacaaaca ataaggattt
    44761 aattaaaaat gtgaacataa ataaagatac aaaaatatac tgggtggaag gcgaaaataa
    44821 gtggcattta tataaggatt gtccattgat aaaagaagga gagattctaa aaaatgacaa
    44881 acttttagat aggtctacta attatctatg taatacttgt gtacttagag ctgcaacaga
    44941 aaaagaagca aaaaaagaag tcgtagctcc aaaggcagaa ggtgtagtag atgctacatt
    45001 taaatttata atgtacacta aagagaatac tacaatgata cttgatacag ataatccatc
    45061 ttcatggaga ccaggaaaat ataatccatt taagtctagt gctaaataaa gctaagtaag
    45121 ctttaaagat aggtgatgag tttgtacaaa aataatgaaa aaggtcttac tttattagaa
    45181 gtaataatag ctgtttttat actgacaata gttttaagta tttcttataa agtgttcaat
    45241 ggtataacat cagcagtaaa aaagcaacag attattacag atgctcaagt aaatattaat
    45301 ctaattaata agtatctaaa tagagatttg gaaaactgta aagagctaac taaaactggt
    45361 tcaggtaata attatgaata caatatagag atgccagata atgttgtaaa atatgaagtt
    45421 tctatagaaa ctaaaaaaaa tacagaggta tattctgtaa cgagaatcca aaacaataca
    45481 attgatacag aaaatgaagt tagagaagag ataatctata atcaaccact agttcaaaac
    45541 aataaagaaa tgaaggaaac accatttaaa atagaaaagc aaactggtaa atctatttat
    45601 actgtaagta tatactataa tgaatcagtg caagaaagtc ataaaaatag caatttaaat
    45661 aacaaaactt atacatttga tgttatgtca agaatagggt aataagaatt tattaaaaaa
    45721 taagtaggtt gataaaatat gagtaaaaag aatagtgaaa gaggattttc cttaattgaa
    45781 gtgttagtag ctatggctat tatgggaatt gtactatttg cattttttaa tattatcaat
    45841 actaataaca aagcaaacac taaaaatgac acagatataa cttccttaaa ctatgtccaa
    45901 agtgaaatag aaaatctaag agaaaagata aaaagtggag aatttgattt tgatagttta
    45961 gataaactag aagatggaac tgtcgtatat gaaaaattaa tagataaatc aaaaaaagtt
    46021 gtatatgata aagttcttag tgaaggtgac gtgagcttat atgatactcc atatgaaaag
    46081 attacaacaa taaaagatga agatggtaat ttaatcgaca aaggaaatat tacaaataag
    46141 ataaagacta ttgtagaaga taaaagtggt cagatatata aaatagctgt aactgggaaa
    46201 agcatgaatg actattcaag taaaaaagag gttaaaattg taactgaaat atttaaagat
    46261 aaataatttt acttgggggt gagattttga ggaagtggaa taaatttaaa agtgaaagag
    46321 gggcagcttt agttttagtt ttgattgtag tagcattgct ttctattgtt ggattgatat
    46381 tttcaaatca gatagctaat agaataaaat ctacaaagac tactaatgaa ggtatacaag
    46441 caaagtattt agcagagaca tgtgtggaaa atagtattga taaagcatat gaaaaattgt
    46501 atgatgagct tgagaaaatg gataatgagt ttaaaagtga aaatcaagaa aaatccatat
    46561 ctaggtcaaa attaagaaat atatcagatg aagattttaa taatcaagat gagaaaaata
    46621 ttgaagcaga aagacttgga tatatgaata atataaattt ctatttaaat aaagcttctt
    46681 cagacttgga aaaagctagt atggaattga aaaagctata tgatttagat atgttggact
    46741 atagagacat tgaatatgta gatgcaaata ttatttcaca tagagattct atcttagaaa
    46801 tttgcaagaa ttatacttct ggagatatta gtaaaataaa tgaatatatt ctgaaagaag
    46861 atatagactc aactacattg attgaggcaa agttagtgaa taatgatatt cttttgaaaa
    46921 tgtttttaga agaaaataaa attgaaaatg aacatttaaa ttcagccttt tcacatactt
    46981 ataaggcatt agataacatt tctttggcga tgcaaaatat gattgagtat agacatacgt
    47041 ttcatataga tgagccaaaa gtagaagtat ctaatgggat tccagattct caacaatatt
    47101 atgaactaat acaaaatcct ataataaata gtatggagta tatctggaat agtaaatggg
    47161 atacattaga aaacctattg gaaattttac caaatcaaac tcaaggattt aattctttaa
    47221 gagttcattt gagaaataat gtaagaaaat ttgagaagtt aagcgataat atatctagtg
    47281 gtaaaaaaaa tacagcaaaa aattttttaa agtataaaga gttattgtat gaaatatcag
    47341 accaatgtaa tcaactaaaa agtatgtctt atgagaaaat acctgttaaa tatgataata
    47401 tggctttgat aacaactttt gactatatac aaaatgagct tttagcagag ataaaatgca
    47461 gattaaaaga attaaaacca caagaaattg ataaaacaga aggtattact ataaaaatac
    47521 ctttttataa ggctgactac gacatgacaa aagaaggatg gcctaaatta aaagaaaatg
    47581 gttcaggggc ggagttaagc ttaatggtaa ctggtgataa agatggaata aaagaagtag
    47641 aagttacaga tggtaaaaaa aatattattg gtttaggtgt tgaagaaaat tcaaattcaa
    47701 agtataaggt tgacgccatt gtaaacttta atctaaatat agatacaaat gtagttggaa
    47761 attatgatat aaaagataaa attttgatta atcatgatat aagttcatac aaaaaagtaa
    47821 actaaatata agaagcaact tagtataaat aaataataaa ataattttat tatttactac
    47881 taaaaaaata agtaaactaa tagaagatat ggagagtaaa tattatgtat atttatgatt
    47941 tattagagca aggtataaga ctaaacgcat cagatataca cataacagtt ggaacaaatc
    48001 ctgtagctag agtaaaaggt ggatttgtaa aactaagtga acaaatattg acatcagaag
    48061 taacaatgca aatggcaaaa gacatagcgg gggaatctat ttttaaagta atagaagaac
    48121 atggagaagc tgatttttca gcttctctaa aaacaggaga aagatttagg gtaaatgcat
    48181 ataggcaaaa aggaaattac gcaatagcaa taaggactat aacagcagaa attccaacat
    48241 ttgaaaaatt aggccttcca gagagtataa aatcttttac tgaaaagcat aaaggattag
    48301 ttttagttac aggtccaaca ggaagtggta aaagtacaac acttgcaagt atgataaata
    48361 taataaatga aaaacaacaa aaacatataa ttacacttga agacccgata gaatatgtac
    48421 atcatcataa acaaagttta gtaaatcaaa gagaagtagg aactgataca gaaagttttc
    48481 attcagccct tagagctatt ttgcgtcagg acccagatgt aatactaata ggagagatga
    48541 gagaccctga aactgtttca atagcattaa cagcagcaga aacaggtcat ttagtatttt
    48601 caacactaca tacagtaggg gcagctaaga caattgatag aatagtagac atgtttcaac
    48661 catcccaaca gcaacaaata aaaacacagt tatctacagt ttgtgaaggt gtggtatcac
    48721 agcagttact tcctacagca gatgggaaag gcagaatagc agcaattgaa ttgatgtttg
    48781 ctactccagc aataaaaaac ttgataaggg aaggcaaaac atatcaaatt ccaaacatga
    48841 tacaaactgg agttaaagta ggtatgaaaa ctatggacca agatttaatg gaactttata
    48901 aaaatggaaa gattacaaaa gatatggcac ttagtaggtg tactgaccaa gagtttatga
    48961 caagaatgat aggtggagta aactacaatg gatattataa tcgttaatct atatgtattt
    49021 atagtgggta tagtgtttgg aagtttcttt aacgtctgta tttttaggat tccagaaggt
    49081 atatctattt ctagacctcc ttcccattgt atgagttgta atactagact aaagccaata
    49141 gacttaatac cagtgctaag ctatttgatg agtggcagaa agtgtagata ttgttatgag
    49201 aaaatttcta gtagatatgc tatagtggaa ttaattacag gaattttatt tttaacagta
    49261 tttatgttat atggaatatc tacatcaact atttattatt tggttcttgt gtctttactg
    49321 attataatta catttataga tattgaccac tttataatac cagataaaat attgattttt
    49381 ggagctatat tttctatgat ttttaacctt ttatttaaag aaattccagt taaggattca
    49441 attctaggtg tttttatttg tggaggaagt gtctggatta tagttctttt gattgaattt
    49501 gtaataaaaa aagagtgtat gggtggagga gatatcaaac tatttgcaat gctaggcctt
    49561 tacatgggtg ttaaaaatgg tcttttgaca gccttactaa gcgtttatgt aggagcagtc
    49621 tatggaatct gtgtaataat tataagtaga ataaaaggaa aggaatataa ttctgttata
    49681 ccatatggac cttttatatc tattggagca ttaataacaa tcttatgtgg aaagcaatta
    49741 cttgaacttt atttttctat ggttatatag atgatagtca agttaaataa agttttaatt
    49801 taaaaaaatg aattaaagta tttaataagg acaatattat tattaaccct attactaaag
    49861 tgctacaaac tatggagtat ataataataa tttccttatt ttttgcacat ttgataaata
    49921 aatctctatg tgttttttgt gatagagaaa agaaaaattt atataatacg ttttttattt
    49981 acataataac tgtagtttta tctgtattaa tatatctaaa atttgggatt agtgtagaat
    50041 gtgtacaata tatggtttta attccatttt taataattat atccattgta gattactaca
    50101 caatatatgt atatgatata acaatagtta gtggtataat agttcaaggt ataatttttt
    50161 tggccttttt caatatagct caatttgatt atataaatca tatatttgga ctattgatag
    50221 gctttattat accttatatt attgctaaac taacaaaagg attaggctat ggagatgtag
    50281 gtctctatag cttatgttgc tttgcattgg gaaataatta tgggttttat gtgatagttt
    50341 tatcatttat tttagcgttt ttgtttttta ttattttata cataactaaa atatttaaag
    50401 atgtagatat aagaaagcaa attcctttta caccttttat ttccttgtca acaattttaa
    50461 taatatttac agagtataat atatttaatt tttattataa tttgttatac aaatttatat
    50521 aacaggaggt aagaaatgta cgtagtagta gggttaggta atccaggaaa gaagtatgag
    50581 aagactagac ataacgttgg atttgatgtt atagatattc ttgctaaaga atacaatatt
    50641 agtgttacta aaataaagca taaagctttg attggtgaag ggagagtagg tactgaaaaa
    50701 gtattattag ttaaaccaca aacttatatg aatttaagtg gagaaacatt aatagatata
    50761 tataaatatt ataaggttga tttaagtaat atagtagtag tatatgatga tatagattta
    50821 gaagttggaa agatacgtat aagaaaaaaa ggaagtggag gaactcataa tggcatgaaa
    50881 tctataacta agtgcctagg ttcaaatgac tttccaagag tgagagtagg tgtatctaaa
    50941 ccagaagctg gtcaagactt agcagacttt gtactttcta gatttagaaa agaagaatct
    51001 gataacataa atgaagcatt agaaaaagcg gcagatgcta ttgactcaat aataagagaa
    51061 aatatagata tgtctatgaa caaatataat ggatagtgag agggagattt aaatgaatga
    51121 tgttttttta tatcctttgc aaaattcaaa agagtataaa gatataataa actgtataaa
    51181 aaatacaaaa ggctcattat tggttaatgg acttttacca gtacaaaaac ctcatatatc
    51241 gtattctata tttaatgatt tatcacgaca aatgattttt ataacgagta gtgatttaga
    51301 agcaaagaaa gtatacgaag atttaagttt ttatatggaa gataaagttg aatatttagg
    51361 ttttcaagat atttattttt atcacttgga tgcaaaggac agaaacgaag aagcaaagaa
    51421 attaaaagta ttgttaaaac tagcaaataa agaaaaaatc attttagtaa cttctattga
    51481 ggcagtttta agaaaataca taccaaaaca agttttgctc gatagtgtat ctcattataa
    51541 ggtgggagat agcttagact tagaaaagtt aactgagaaa ttagtttcat tgggatatga
    51601 aagagtttca aaaatagagg ggtttggtca atttagtata agaggtggaa ttattgatgt
    51661 attttcactt gaatatacaa atccaattcg tatggaatta tttgatgatg agatagattc
    51721 catacgaact tttgacgtat attcacaaaa atcaattgat aaattgcaac aattctctat
    51781 aactccatct agagaattta tttatcccga aaaaactaca gatgcattag ttaaattaaa
    51841 aaaagaaaca actaaaaata ctgatgaagg tacatttcaa aatatagatt atatatcaag
    51901 taagacttat tttgaaggtg cagagaacta tatagattat atttatccag aagaaaacaa
    51961 aagtatattt acttatttag cagatgatgc aatagtattt attaatgata taacgagact
    52021 taaagaaaga tgcgaaaatt atataaatga gtttagagaa aattataaat taaatcttga
    52081 aagaggtctg gctattaaaa accaaggtaa attgttatat cactatacag atttagagta
    52141 cttagtaaaa gacaagtctg tggtattaaa ctcgctattg ccaaagtcta taaataactt
    52201 ttctataaaa tctataataa attttgaaag tagagaagtt cctacattta atggaaaggt
    52261 agatttgctt gtagaagaat taaatcggct gaaatataat ggatacaaaa taattttagc
    52321 aacaaatacc ttagaaagag caaataaatt aggaaaagat ttattggaca aaggcttaga
    52381 aacgaccatt tctaaagata gagatattga aattaaatct tcacaagtta taattgtacc
    52441 tgcacacata aacagtggtt ttcagtataa atctataaag tttgttgtta taacagataa
    52501 cgaaatgata ggtgtttata aaagagcgtc taagactagc aataaaaaag ttaaaaaagg
    52561 aaagaaaata gaatcctttt tagatttaag tgttggagac tatgtagttc atgaaaatag
    52621 tggtgtaggt agatacacag ggattgaaca aattacagtc aatgctatta aaaaagatta
    52681 tatgaaaata gtatatcaag gtggagataa tctatatgtg ccaatagacc agatggacaa
    52741 agtacaaaaa tatattggtg cagaagtaga aaaagttaag ttaaataagc ttggaacaaa
    52801 tgagtggacg aaagcaaaag caaaagttaa aaaagaaatt gaagatatga caaaagactt
    52861 gattgagctt tatgcaaaac gtgaaaaaat acaagggtat aagttttcaa aagatacacc
    52921 ttggcaggca gagtttgaat cgttgtttcc atatcaagaa acagaagacc aattaaaggc
    52981 tatagaagaa actaaaaaag atatggaatc aagtaaggta atggataggc tagtatgtgg
    53041 agatgttggt tatggaaaaa cagaggtagc tattagaagt atttttaaag cttgtatgga
    53101 ccaaaaacaa gttgctgtat tggttccaac aacaatattg gcccaacaac attataatac
    53161 attcaaagag aggtttgaaa attatccttt gagagtagaa gttttaagta ggtttaaaac
    53221 accaaaacaa caaaaacaga ttattgaaga tgctaaaaaa ggattagtag atattttaat
    53281 tggaacacat agaataatat ccaaagatat aaatttacca aacttaggtc ttgtagtcat
    53341 tgatgaagaa caaagatttg gagtaaaaca taaagaagct ttaaagaaaa ttaaatctac
    53401 agtagatgta cttacacttt cagcaactcc tataccaaga acattacata tgtctttaag
    53461 tggaataaga gacatgagtg ttatagaaga acctccacaa gaaagacatc ctgtaataac
    53521 atatgttaca gagagtaagg aaagcgtaat tcaagatgag atagagagag aattaagtag
    53581 aggtggacag gtattttttg tatacaatag agttgaacac attgaagaaa tggcaagtat
    53641 gattcaaaaa ctcgtaccag atgctagagt ggctgttgca catggaagaa tgacttctaa
    53701 atcattagaa aatataatcc taggattttt aaataaagat tatgatgtac tagtctgtac
    53761 tacaataatt gaaactggta tggacatatc aaatgcaaat acaatgatta tatatgatgc
    53821 agataaaatg ggattggctc aactttacca attgagaggt agggttggaa gaagttcaag
    53881 acaaggatat gcatatcttt tgtatgaaaa agataaaact ttaagtgaga tagcggagaa
    53941 gagattaaaa gctatcagag aatttacaga gtttggttct ggttttaaaa tagcaatgag
    54001 agacctcgaa ataagaggag caggaaatat tttaggttct caacaacatg gtcatatggc
    54061 agttataggt tatgatttgt acgttaaaat gcttaatgat gcaattaaaa aagtcaaagg
    54121 tgaacctatt gtggaggaaa ttgatgttga aatagattta agtgtaaatg catacattcc
    54181 agataattat atcaaagatg agttgattaa aattgaaatg tataagaaaa tagcttctat
    54241 tgaaaacaaa gaagacatgt tggatataca agaagaactt gaggatagat tctctgatat
    54301 acctaaacca gtccagacac ttcttacaat agcttatata aaatcgttat gtaagatatt
    54361 aaaaatagaa aagataagac aattaaaaga tgaaatatta ttagttccaa taacaaagta
    54421 tagaactaaa cagaaaatag gttataatat agttacagaa ttagaagaat tattagaaaa
    54481 gatgtgcaag gtaaaataac ttgtaaattt aagaagggat gttttaaatt tgaaaaaagt
    54541 aataacattg gtaatagcta tgattttggt agtatctgtg actgcttgca gttctagtaa
    54601 aggagaaact gtggcaacag ttgaaggtac aaagatatca tcagatgaat ttaaaaaaac
    54661 tatagctctt tataaagatt ctatggaaca aacttatgga aaagatatat gggataagga
    54721 agttgaaaag ggtgtaaaat acaaggacaa gtttaaagat ttaattcttg accaacttat
    54781 aactacagaa gtaatatatt ctcaagctaa aaaagataac ttacttccta aaaaagagga
    54841 tgttgaaaaa agttttaaag aattaaaaga cgctatgggt aaagatgaaa aatataaaga
    54901 acaacttaag aaattaggta tagatgatga gtttttaaga gaccaacaag agaaagattt
    54961 agcaatgcaa aactatcaaa gtaactttgc taaaaagact aaaatatcag atgaagaaat
    55021 gaaaaaatac tatgatacac ataaagatga atttaaaaaa gatgaggtag aagcttctca
    55081 tattttattg aaaacagtag atgataataa caagccactt tcagataagg aaaaagctga
    55141 agctaagaaa aaggctgaag aggctcttaa agaagttaaa tcaggtgagg attttgcaaa
    55201 agtagcaaaa aaatattcac aagatacttc agcaagtgat ggtggtaaat taggattctt
    55261 ttcaaggggg caaatggttg ctgaatttga agatgctgct ttttctatga aaaagggtga
    55321 agtatctgat ttagttgaaa ctcaatatgg ataccacata attaaagtga cagacagaat
    55381 aaatgagcaa acatcatttg aagatgctaa agaaactata aaagaccaac tgttgaaaaa
    55441 taaatatcaa gaacaaatag aaaaattaac taaagaagct aaggtagaaa aagatgaaaa
    55501 agtaataaat aaaatcacaa tctaagactt aaaaaaagag taatatgctt aaaaaagcta
    55561 tatctggata agatatagct ttttttgtat atttttctta acaatggtaa aaataaactt
    55621 aaagtaattt atggagggat taaaaaatat gaaagcaaca ggtatagtta gaagaataga
    55681 tgatcttgga agggtagtta tcccaaaaga gataagaaag acattaagaa ttagagaagg
    55741 agacccttta gagatattca cagcaaaaga tggagaagtc atacttaaaa aatactctcc
    55801 aataggtgaa ttaaatgaat tttcgcaaga atatgctgaa acattagctg aagtactagg
    55861 atatggagtg ttgataacag acttagattc tataatagct gtgtctaagc ttcctaaaaa
    55921 agattataaa gaaaaaagta taagtgatga gttagaagca ataataagtg aaagaacagt
    55981 aaaatattca aaagataaaa acattttacc attattcaag gaagaccaaa aagaatatac
    56041 tagtcaagtt atcatgccta taattagtgc atctggtgac tgtataggtt caattgtagt
    56101 tgtttcaaag gataaagata caattgcaga aagtgaagaa aaatctttaa aaattgctgc
    56161 taacttttta ggaaaacaag ttcaataatt ttatcataaa gaccttttac atgaaggtct
    56221 tttgaagttt ttgtgtaaaa tattgaatat agcattaaaa atatggtata ttaaagtttg
    56281 ttatataaac agtatggagg ttatgttatg aacaataaca aaaataaaga ctcctttcta
    56341 aaaggagccc ttatacttgg gctagcaggt gtaatcgtaa aaataatggg tggatttttt
    56401 aggattcccc ttggaaacat gattggttca gaaggaatgg gatactatca ggctgcttat
    56461 cctgtgtata ctctattttt aacattagct actgctggat ttccaacagc tgtggcaaaa
    56521 ttggtttcgg aaaaagttgc aattggaaat tttaaaggtg caaataagat atttaaggta
    56581 tcacatacag ttttattcat taccggtata atatcatttt gtatattatt ttttggagcc
    56641 gactacatag taacaaacgt aatgaaaaat ccaggtgcgt tatactctat gaaagccatt
    56701 gctccagcat tactatttgt tcctgcaatg tctgcatata gaggatattt tcaagggaga
    56761 caagatatga ctaagattgc tgtgtctcaa gttgcagagc aattttttag agttgtatta
    56821 ggtcttactt tggcctactt gcttatgaag agtctaggtc agacttttgg tgcagcaggt
    56881 gctatatcag gggctacaat aggttcaata gcatctatgc tttatttggt gtttgcttat
    56941 atgctaggga gaaaagagcg tagagcagag atagatgcaa gccaaagatt taaagatgaa
    57001 agagtgtctt atatttttaa aaagctttta actgttgcca ttcctataac aataggagca
    57061 tcagtaatgc cacttgtaaa tatgatagat aatgttattg taataagaag acttatggaa
    57121 gcaggattta catacaaagt ggcaaattca atgtttggtc aattaacagg tatggcaatg
    57181 gccacaataa atttaccagc agtcataaca actgcaatga gtatgagctt agttccagca
    57241 atttctaaag catatgcatt aggaaataag tcaaaagcta gaaaagatac taagagtgca
    57301 gttaaggtaa ctttacttat agtattgcct tgtgcgtttg gtatggcttc tttagcaata
    57361 ccaataatgg gtcttttatt tccacatgaa ccatcttctg taggaactat tttgtttact
    57421 cttactccat gtgtattatt tttaggatta atacagacat tgactggtat aattcaggga
    57481 atgggaaaac caatagtacc tgtaattgct ttatgtgttg gtatgctatg taaaattgta
    57541 ataagttata ctttaacagg tataccagat attaatgtac tgggttcagc atttggtaca
    57601 gttacagcat actttgtggc agccatgatt aacttactat atgttaaaaa acacatgaat
    57661 gtaaactttt ctaaaaaaga atttataata aagccattta taacagttat gacaatgttt
    57721 ataatggtta agttgagtta tggtgcttta gttggattct taggaaacag catatctaca
    57781 ataattgcaa tatgtgttgg tggaattgta tatgtagtag taatacttgg aataggtgga
    57841 attaaaaaag aagagattct aactatgcca aaaggtgata aattatataa attgttaaaa
    57901 aaagttaaac ttataagata atataggaat atatatgcaa ataaaggaaa tactatacaa
    57961 atagtaaatc tccttatttg tataataata taaaaataaa gtgaaatttg ctaataggag
    58021 tgatgttatg ggtaagataa gtattgtagg tcttggacca ggagactatt ctcttataag
    58081 ccaaggtgtg ttagaatctt taataaatag ccctaaagtt ttttttagaa cagagaaaca
    58141 tccaataatt gataaactta aagaaactat acaatacact tctttagact acttttatga
    58201 aaaagaggaa aattttgata atgtttattt gaaaatatct gaatttatca tagaagaatc
    58261 agcaaagggt gacctagtgt atgctgttcc tggtcatcca agagttgcag agaagactgt
    58321 aagtataata gagagattag ccaatgaaaa gcacatagca gtagaaatat tgccttctat
    58381 gagctttgta gacgctatgt ttaactattt ggctatagac ccatctgagg gatttaaact
    58441 ggtagatgct tttgaaatag aaaattcata tatagacttg gatacaagca tgataataac
    58501 tcaggtatat gataaattta tagcatctaa tgtaaagtta aaacttatgg aacattatga
    58561 ctataatcaa gaagtatgta tagtaaatgg agcaggtgtc aaagatttag aaagtaaaaa
    58621 gtttataaag ctttgtgaat tagacagaaa tgaaaacttt tttgactatt taactagctt
    58681 atatataccc aaaagttcta aaaagatgta taatacagta catgacttag aaataatagt
    58741 taatacactt agaagtccat ctggatgtga atgggataaa aaacaaacac atcaatcatt
    58801 aaaaaattct gttatagagg aagcatatga actttgtaat gcaattgata ataatgatat
    58861 agatgaaatg gtagaagagt taggtgatgt tcttttacag gttatattcc attgtcagat
    58921 aggaaatgag gagggctact ttgatttaaa agaagtagta aatggcatat gtaaaaaatt
    58981 aattcataga catcctcata tatttagtgg tgcaaaatta gatatgtcta agtttgagaa
    59041 aacttgggaa gaattaaaga aagaagagaa aggcgaaagt actataacag atggccttag
    59101 aaggatacca aaacatttac cagctcttat gaaggctagt aaagttcaac ataaagcagc
    59161 acttgtcggc tttgattggg acaatgtaga agatgtattt aaaaaaattg aagaagaata
    59221 tcaggaatta cttgacgaac acagggtcgg gaatataaaa tacataaagg aagagctagg
    59281 cgatttatta ttttcgatag taaatctagc tagattttta gacatagact caggagaagc
    59341 cttgaactgt actacagaaa aattcataaa tagatttgac tttatagaaa aaagtgcaat
    59401 gaacttgaat aaaaaattag aagacatgac acttgaagag atggatgagt tttggaatca
    59461 ggctaaaaaa caataaaatt gtggaaggaa atttgtcaaa aatctagaac ttttatcaca
    59521 aagatagaag tctatattat aaacattttt aggaggtaaa tatcgtgaat aaagctgaat
    59581 tagtatcaaa gatggcagaa aaaagtggat taacaaagaa ggaagcagaa gctgcgttaa
    59641 acgcatttat gagttctgtt caagatgcac tagtaaataa tgaaaaagtt caattagttg
    59701 gatttggaac atttgagaca agagaaagag ctgctagaca aggaagaaat ccaagagatc
    59761 cagagcaagt tatagatata ccagcttcta aagcaccagt tttcaaagct ggaaaaggat
    59821 taaaggatat aataaatgga taattagaaa tcttgtgaaa aagtgtggat aaatttgcca
    59881 cactttttct atagaaccaa ggaaggtgat aattatgaga ttagataaat ttttaaaagt
    59941 atctaggatt ataaaaagaa gaactgttgc taaagatgca tgtgataaag ggattgtcac
    60001 tataaatgga aaagttgcta aatcttcttc agaggtgaat attggagata cattagaaat
    60061 acaatttgga gagaagaaga tgaagtttaa gataaatgaa gtaaaagagc atgtattaaa
    60121 aaatgatgca aaagaaatgt acgaaatttt agaataaaat taatagtcct ctagtatatt
    60181 aatagtagta ttattgaaat atttactggg gggatattat ggaacagaac ataactttga
    60241 aagatagatc aaagctggtt atttcgggag tcgagcacat atactctttt aatgacaaaa
    60301 gagtagagct caaaacttct gtaggcgaga tggtaattga aggcgaaaac ctagatatga
    60361 gcaagctaag tatagacgaa aatataatta gcatagatgg aactataaat tctatggtat
    60421 atgcaaagcc taaaaagcca caagagagtt tcttgaaaaa ggtatttaga tagatgatac
    60481 cttttactca agatgtaagc attttttatg ctactattta tggtggaata ctcatagggg
    60541 tgttgtttga tttttataga gggctaagag ggaattttaa attcatcaat tattttgcta
    60601 taatctttga tgtattgttt tggtttctag ctacggttat aatattcgta acaataaacc
    60661 ttacagaatt ttttgattta agatattatc attttgtggc tttgtttatt ggttttattt
    60721 tatactataa caccataagt aaaattgttt tatcaattat caataaaata attcgttttg
    60781 tgagaaattc ttttaaaaaa gttacacatt acatagtcag ttttttaaat aatttgtatt
    60841 acgttataat ctattcacta cacctactat ttgatattat attttatata ccaaacatat
    60901 ttatagcaac aagaaaaagt ataaaaagaa aatcaaataa aaaattaaag aacaagaaaa
    60961 aatcaaaacc taagaagaaa aaaaataaaa ctaaaaaaag ggtgtaggaa aaatgaactt
    61021 aagaaaaaaa ttttcaggac agattattgt tatcagttta tttttaggaa tatcaatttt
    61081 tagtatgatg acaggatttg tatttgagta tacaaaagct aaggaatata aaaaagaaat
    61141 agcaagtcta aataaacaat taaaaaagac tgaaatacaa ataaacagct taaagaaaga
    61201 tgaaaaatca tatgaaggag acttagagga tatagcaaga aaaagattga atatggtaaa
    61261 accaaatgaa acagtttatg tagacataaa tcgttgatta aaacttgcta ataagtttac
    61321 agatgtattg attttaaaat ttaagattta ataaaaaagt cattcagtta aggatggctt
    61381 tttgttttta ttgaggaggg aatttcataa tgaaagtagg agctatagat attgggacaa
    61441 actcaatgag attactgata gcagattata gagatgggaa ttttataaac agaaaaaaat
    61501 ttgttaatac aactagaatc ggttatggtg tggatagtaa aggatatata aaagaagatg
    61561 ctatcaaaag aaatataaaa gcattaaaag aattttctga taagtgtaat gatgaaaagt
    61621 gtgaaaaaat ttattgtatg gggacatctg ctcttagaga cagtaaaaat ggagattact
    61681 ttgttaaatt agctaaagaa gaaactggaa tagatgttga tatagttacg ggaatagaag
    61741 aatcaaattt gggctttaag ggtgttttag aaggaataca aggtgatgat actattattg
    61801 ttatagatat tggaggaggt tcaacagaat ttattgtagg tgattcagaa ggaattaaat
    61861 ttaataaaag tgagaatgta ggtgctctta ggatgacaga gaggtttttg aagcacgata
    61921 ttattgaaga tgaagagttt gaagctatga gttcatttat atatgatgag attgacaaaa
    61981 cgttaaatca tataaaagac agaggattaa aaactcttat aggtatagga ggtacgatta
    62041 catcattatc agcaatgaat caagaattag agatttactc tatggaaaaa atacataata
    62101 gtaaaataca caaaaaagaa atagaagaaa ttctacaaaa attaataaag atgacattaa
    62161 gtgataaaaa gaacttaaaa gggctacaag aaaaacgtgc ggatattata actgctggtg
    62221 taaaaatatt gaatataata atggaaaaat tagaaataga agaaataacc gtaagtgaat
    62281 acgataattt ggaaggctta atatgtcaaa agtcaaaaaa aatgtattaa tattttttga
    62341 tttttaaaac cgattatgac aaaaaaaatt actcctcttt gttacaattc ttaatataaa
    62401 caaaagagga ggttttttta tgcaaagaag tgtagcagtt gtgaagaata agactgtaaa
    62461 accaaatgtc ataaaattaa taagcggata tattgatttt actacggcgg cattcgctgt
    62521 gatgggattt ttacttagta ggtctattat agtagactct gtagctcctt taggaattgc
    62581 cttttttata tgtgcatcaa agattgataa gtacaagata cctgtattca tatcaacatt
    62641 attaggaata ttgttatcat ctaatgattt tggaaatgta gcaaaatatg ctgtgtgcct
    62701 tacaatattt atggctatga ataaaaaatt aaaattatca gattcaattg ctaaggttgc
    62761 atttataggt gcaataatac tacttccaat ctccatggga caagctttct tttcaaatag
    62821 gtatgtatat ggtcttctta tagcaggagt agaagcaata ataatgttta tatctatata
    62881 tgtgttttct tatgggatag gtttaatggc caatgtcaac aataggatgt ctgtacggac
    62941 agaagaagct atatcaataa gtttactgct aacctttagc ataatgggaa taggagatat
    63001 atctctgttt ggaatatcaa taaggaccat tttatcgacc gtattgatat tgctgacctc
    63061 aatagtaggt ggagctacca tgggagcatc tagtggtgta attattggtg tagcatccat
    63121 tttaaataat atgacatcag ctgtttatat ggggatatat tctttttcag gattaataag
    63181 tggagcattt aataaaataa ataaatattt ctgtatacta ggatatattc taagttggac
    63241 aataatatac ctatatactt ctggtattac ttctaatatg atgcaattaa gagatatact
    63301 attaggttgt ttgatagtac tagtattgcc agagagatta tttaataaga ttgaaaaatt
    63361 aataaaaagt aatgtggctt caaatgaaat cgtatacgat tatattatga gaagcaaaaa
    63421 cctaactaat agcagattga acagtattta taaaacttat gatgaccttg cagatacatt
    63481 tgataagata agagaaaaag acaaagtgct agaccaaaga gatatagcta atgtaataga
    63541 tatgattcat aatgatgaat gtaaaagttg tagtatgaga agaatgtgct gggagtcaag
    63601 atttaatcat acatacacta tgatatataa cattcttgaa aaaattgagg agaaagggga
    63661 gttaagtcta aacgatatac ctaaaaattt tagaaaggaa tgtatgaaac cagagtcgat
    63721 agtgaaaata tcaaatcact attacaaaat gtttgtatta gattatgatt ggagtgtgaa
    63781 gttttcagaa agtagaaagt taatagcaaa tcagataaga agtatttcaa aatccattaa
    63841 atccttatct caagatttag aaggtgacat aatgctagat atagaaaagg aaaaaaatat
    63901 atatgaacag ttagaaagat atgatataac agttgataaa gtaagttact taacaaaaag
    63961 taacaatgag tttgaaatat caatagaaaa gaaaacttgt catgatggat gtatgtgtga
    64021 agataaaatt gtaaatataa tttcagacct agtaggtgaa aatatgtctg ttagaaaaat
    64081 aggatgtcac tgtttaggag gtaaatgcaa ggcaacattc gttaaatctc aaaaatataa
    64141 ggctgttaca gaagtttcag caatgtcaag ggatgggcat atcctttgtg gagacaatta
    64201 cacatacatg gagataaatg atggtaaata catgatggct ataagtgatg gtatgggaaa
    64261 gggtaaaaaa gcatatgaag aatcttctgc cactattgat atactggaaa aaatgataga
    64321 tgcaaaaatt aaagatgaga tagtcattga tactataaat aatatgttgt tgttaaaatc
    64381 ttcagaagag atgttttcaa ctctggattt gggaatttta gatttaaaac gaggttgttt
    64441 agaaactata aagatgggag cgtgttctac ttatataaaa agagaggatg gagaagttga
    64501 cttaatatca tcatcatcat tacctgtggg tatattatca gatgtaaaaa ttgatagaaa
    64561 aaatgtaaaa gttaaggaag gagactatgt tataatggtt tctgatggaa ttgtagatgc
    64621 aggaagaaat aacaatttag gagacaattg gttaatatac ttcttaaaga atatagagac
    64681 tacaaatcca aaggaaattt caaatttaat attggataga gcattagagt tacaagcgtt
    64741 acaaatcgaa gatgacatga ctgttttagt tacgaaaata tgtacaaaat aaggtttaca
    64801 agattatgtg tataagatgt ggataacttg tgtataatat gttaatagac ttttgaaaaa
    64861 agtatagtta ttaacaaaaa taacatataa atcaacaaaa tttgttgata agtatggcag
    64921 aaatgttaat aaaatgtaaa aaaagaaaaa acaaaagcta ttatcaaaag agattcatgt
    64981 tgaaataact aaatttctaa gtaaagaaaa cctgtgaata tgttgattat tatgtggata
    65041 actattaatt cgatatttat ttatatagta tgaataattg aataatatat ctaaaaaaag
    65101 ccatcctatt attaagtaaa tttattgaaa ggatggcttt ttatgaatag aaaattgtgc
    65161 aaagcaataa tacctttatt tatttctatt ttttgcatgt taataataag tataaaagct
    65221 gaccaaggat atcaaaagaa taatgacaga gcaattgctg tcatgaataa taatgatact
    65281 gaagatgatg ctgaaaaaaa acaagaagta gactatgaaa aaacatgttg gaatttgcaa
    65341 tctttgttca aaagtgatga ccaatggaaa aaagaactta agagttttga taaagatact
    65401 aaagagttga aaaattacat aggtaaagta acaaaatcgc caacacattt gtcttttgcc
    65461 ttaaacatta aggaaaaact tgatataaga ctaaataaac tttctgccta tgtaaaatta
    65521 aaacaagata ctaataaaaa ttcatataag tacttagata tgagtgatag tatgagtaaa
    65581 tcatatggga attatatggg gatttgttca gatttagaat tagaaatact aaaattatca
    65641 aacaaagatt acaagaaaat tatttcaaat aaaaatataa ataggaaata tggtgcatat
    65701 ttaagagata taagaaggaa taaagtacat tatttggatg ataaatcaga agatattcta
    65761 agcaaagtat cttcaataag tagtcttcca agccaagtat atgaattatt tagaaatatg
    65821 gatagaaaaa ctaatttgac accagcgcaa tatgcaagtg aattagagtc agtagataga
    65881 gaaaatagaa aaaaagctta tcaagatgaa cttataactt acaatgataa tataaataca
    65941 attgcaggtt taattactgg tcaagttaaa aagaatattt tttattctca ggaaagaggt
    66001 tatgagtctt ctttagaaat gtacttggaa tctgataatg tagatgaaaa agtatacaat
    66061 aatttgataa ggactgtaaa tagtaatatg gacagtctac acaaatatat agatttaaga
    66121 aaaaaagtat taaaattaga taaggttcat tcatatgata tgtttgtgcc tatggttaag
    66181 gctgttgata ataatattaa ttatgaaaag gcacaaagta tagtatattc agcgttgtca
    66241 cctttaggaa aagaatacaa tgatgtagta tataaagctt tcaatgaaaa atggatagat
    66301 gtttattcac atgaaaataa agtaagtggt ggatactgct tatctgttta tgaaaatcat
    66361 ccatatatac ttttaaatta taataattca ttaggttcag tttcgacttt atcacatgag
    66421 cttggacatg ctgtatatga atacttaagt tctaagaatc agaattattt taattcaaat
    66481 ccgtctatat ttactcacga agttgcatct acaacgaatg aagcattact ttacgagatg
    66541 cttataaaag aagctaaagg caatgatgaa aaagcttatt atataacaca gtatttagac
    66601 ttgataaaag atactcttta tacacaaact atgtatgctg aatttgaaaa aactatacac
    66661 gagttagtag aaaaaggaaa aagtgtaaac gcattagtat taaatgatgt ttggggacaa
    66721 cttcttaaaa aatattatgg acaaagctat gaattagacc aattatctaa ggtcggatgg
    66781 gctagaatcc cacattttta taatagtttc tatgtctata aatatgctac aggttgtagt
    66841 gcaggagtaa gttttgccca agacatccta aagaatggtt cagataacta tatgagcttt
    66901 cttaaaaagg gaagttcaga ttatccaata aacttgttaa aatcaagtgg cataaattta
    66961 actaatacaa aacccataga aaatacaatc aaaaaatttg atagtttagt agcagaactt
    67021 gaaaagctat tataaacatt attgtaatcc acatggtaaa tatatcgaat taatgataaa
    67081 atatactaaa agtttaaagt cctatcaaga taggactttt tatttattaa ttagtgggag
    67141 atgattctat gcaagttaaa gttaaaaagg ctgtaatacc agcagcaggt ttaggaacta
    67201 gatttttgcc agctacaaag gcacaaccaa aagagatgtt accaatagtt gataagccaa
    67261 ctctacaata cataatagaa gaagctgtgg cctcaggaat agaggaaata ctaataataa
    67321 caggtagaaa taaaaaatct attgaagacc actttgataa atctgtagaa ttggaacttg
    67381 atttagagaa aaaaggtaag aaagaattgt tggaggtagt acaaaacata tctaacatga
    67441 ttaacataca ttatataaga caaaaagagc caaaaggatt gggagatgca atttattgtg
    67501 cgaggcattt tataggtgat gagccgtttg cagtaatgtt aggggatgat attgtagata
    67561 atgatgtacc ttgtctaaaa caactaacag atgcatatga ggaatataga actacaatat
    67621 tgggtgttca aaaggtaaat caagaagata ctaataaata tgggataata gaagcaaaaa
    67681 atatagaagg tagagtttac aaggtaaaag acatggtaga aaagccagag tcaggaaaag
    67741 ctccttcaaa tattgctatt ttaggtagat atataataac tcctgaaatc tttgatattc
    67801 ttaaggattt accaccaggt aaaggtggag aggtacaact tacagatgct ttaaaaatat
    67861 tatctaagaa agaagctatg tatgcataca attttgaagg caagagatat gatgttgggg
    67921 acaagctagg atttttagaa gctactgttg actttgctct aaagaaagaa gaccttaagg
    67981 aagattttat aaaatatttg aaacatgtat gtagtgaatt tgataaaaat aacaatgtgt
    68041 tatacaataa tacagatgaa aaaatagatt taattgaagt caatgaagct agagaacttg
    68101 aaattcaaaa ataattaatg aaaggtgatg tatggtggca aaatcaagtc agaaaagaat
    68161 taatagaata atagctgttg ggattgtggg aatcctcgta ataacgagta taacagctgt
    68221 tttgagtatg attgcaacta tgtaataaga taaatgacag cagttgatat acataaatat
    68281 aaaaaccata tataacttga tgtagtcaaa aacttacaat attttagtta tatatggttt
    68341 ttagaattta taaatgtaaa aaaaaatttt tataatagtt gcatatttta aaggaaaata
    68401 ttattataag atataaatat agtgataaat ttataaaaac gtgctaagat tggacttgaa
    68461 atacagtaat atagtaggta ataatgtttt aaattttatt tattgaccat aatatataaa
    68521 taatgatatt attagactta actgtttaaa taaaactttt ctttttcatg ccttcaattt
    68581 ccaaaataag tatgtatata aatgaatata tagatgagga ggattttttt gattttagat
    68641 gataaagtat tagaaggatt aggtagtaag accttagttg agctacgaga gatagctaaa
    68701 gaattaaaaa taaaatcaat tactacatat aagaaaaatg agttaattga gattataaat
    68761 tccaaaagta aaaatgagga aaaagtaaac gaagagacta aagaaaaaaa tgaagatatt
    68821 aaagaagaca taaagaatag aaaagaaaat atagagcgtg aaagtataga aaaagaagtt
    68881 gaatataaga gccctaccaa atcatacaac aagaaagaaa tagataccca aaatatttcc
    68941 caaaaccaag aaaatagaaa tcaaagaaat gactataata agacaacgag tgtaaatgat
    69001 agtaataagt catcaaatat atctaataga atggtcagaa ataataataa aaactattat
    69061 atgcctaaac aagttgatga atctaagata gtagatgaat ttaacacatc aaaagaggat
    69121 gaagtagtag gtgtacttga aattctgcca gatggatttg gttttttaag aggttcaaat
    69181 tatttgtcta ctgaaggaga tgtttatgtt tcaccatctc aaataagaag atttaatatg
    69241 aaaacaggag acaagattaa gggaataact agacatccaa aaagtggaga gaaatttaga
    69301 gcacttttat atgttcaaaa aataaatgat gaaaaccctg acacagctat acaaagaaat
    69361 gcgtttgaga cattaacacc aatatttcca gaagaacgac ttacactgga aactaataga
    69421 aatgaaatag ctacaagaat tatagattta atttcaccta taggaaaggg acaaagagga
    69481 ttgatagtag cacctccaaa ggctgggaaa acagttcttt taaaaagcgt tgcaaatagc
    69541 atagctaaaa atcatccaaa tgttgaactt atagtactcc ttatagatga aagaccagag
    69601 gaagttactg atatgaaaga atctattgaa ggagacgtta tatattcaac ttttgaccaa
    69661 gtatcaagtc atcatgtcaa ggttgcagag atggtgttaa atagagctca aagattagtt
    69721 gaacatggta aagatgtagt aattttacta gatagtataa ctagattggc aagggcatat
    69781 aaccttacaa tatctcctac aggtagaact ttatctggag gtattgaccc tggtgcactt
    69841 catggaccta aaaagttctt tggtgcagca aggaatatta gacagggtgg ttctttgaca
    69901 atattaggaa cagcattggt tgaaactggt tctaggatgg atgatgtaat atttgaggaa
    69961 tttaaaggta caggaaatat ggagctacat ttagatagaa aattagcaga aaaaagaata
    70021 ttccctgcaa tagatattta caagtctggt acaagaagag atgatttatt gcttgatgat
    70081 gaagaaaaga ctgctctttg gagattaaga agagaaatga gtaataattc agtaatggaa
    70141 attacagata aagtcattga acttataaag agaactaagg acaataaaga gtttgtaaaa
    70201 tcaataaaga atttgtaaaa tcaataatac aaatgcaaat atggattata taaactaata
    70261 gaaaaatcac ttgaatggca taataagttg tgctataata atgtagttga taatgtaaaa
    70321 agaaagttaa tataattaat ttttttaaat aaagagaaga ggtgactata atgcaaaagg
    70381 aaatacaacc aaaatacaat ccagttgaag tgcgttgtgc ttgtgggaac acatttgttg
    70441 ctggttcaac taaggacgaa ataaaagttg aaatatgttc agagtgccat ccattctata
    70501 caggaaaaca aaagaacata gaaaaaggtg gaagaatcga caaattcaag aaaagattca
    70561 aaatggacta atttttataa aagtggagtt ggaggaagcc aactcctatt ttattacaaa
    70621 aaaacattca tattctactt taaattgtct ttttgtatta taataataaa tatgtaaaat
    70681 tgagtattct aaatacaata aaatgaggtg agtggatggc aaaacaagct gtaggtgggc
    70741 aagctgttat tgaaggtgta atgatgcagt caaaagacaa aagagcggta gctgttagaa
    70801 aaagtgacgg agaaatagca ctaaaagaag atagaataaa aagttgggta agagataaga
    70861 atatagataa gatacctttt gttagaggtt cttttgttat gatagataca atgattcaag
    70921 ggataaaaag tttaaacttt tcttcagagt tttttatgga agaagctgaa gaggataaat
    70981 ttgatttatt tataaagaaa atttttaaag ataaggcaaa tgatataatt ataatatttt
    71041 cattggtcat agcaatgtta ctttctgctg gattattcat ttttatacct acattggtag
    71101 gaggagcttt ttctaaagta atgcctaatg attttatgct taatttaata gaaggaatca
    71161 taaggatagc tatattattt gcatatatag tattgatttc acgaagtaag gatatagaaa
    71221 gagtctttca atatcatgga gcagaacata aatctatata ttgctatgaa aatgatttag
    71281 aacttacagt agagaatgct agaaaattta aaagattaca tcctagatgt ggtacaaatt
    71341 tcctatttat agttatggct gtatcaataa tattgtttgc attttttggt tggccaaatc
    71401 caattcttag aatatttatg agaattatat gtgtgcctat agtagcaggt ttatcttatg
    71461 aggtaattag agttcttggg aaatatgata atggatttac taaaattatt gcatatccag
    71521 gaatgatgtt gcaatatttt acaacaaaag agcctgatga tgagcaatta gaggttgctt
    71581 tggaagcatt gaaagcagta gtagattaga gaaaaggata tttgttatga cgataaaaga
    71641 tataattatt aaatattcag ataaactaaa agatataagt gatactccaa gattagatac
    71701 agagttattg ttacaaaaaa cattgggtgt tgatagatta tatattcact taaatctgaa
    71761 taaagagcta actgaagaac aaaaaacaaa atttatgggt tttgcagaag aaagattaaa
    71821 tggaagacca atagcatata tagttgaaaa tagggagttt atgggattag atttttttgt
    71881 taaggaaggc gtattgattc caagaccaga tacagaaact ttagtagaag aaataattga
    71941 aatatgtaga gaaaaaaaag atgtaagtat attagatata ggaactggtt ctggagctat
    72001 tacaataagt ttagcaaagt acattgaaaa ttctaaaatc atgtcttttg atatatcaga
    72061 gacagcatta gaaatagcta aaaaaaatgc tattataaat gaagttggtg aaaaaataaa
    72121 gtatattaat tctgatttat ttacagcaat aagtgatagt aacataaaat tcgatatcat
    72181 agtttcaaat ccaccatata ttaaaaaaca agatatagaa actctgcaca aacaagttaa
    72241 agattatgaa ccatataatg ctcttgaagg tggagaagat ggattagact tttatagaag
    72301 aataacagaa caaggtaaga aatatttaaa taagtgtgga atattagcat atgaagttgg
    72361 tcataatcaa gctgaagatg taataaatat tatgaaaagt aatggatata aaaaaatata
    72421 cacaaagaag gatattcaag gcattgatag agttgttata ggttacaata tatgatataa
    72481 tcaacaattg atatgttaca tttaatcgag gtgaaagtat gttaaaaaaa ttagaggtat
    72541 tggaagatac atataaagac ttaagtgaaa aaataggtga tccggatgta ataaatgatc
    72601 aaaaagtttg gcagaaatat ataaaagaac atgctgactt ggaacctatt gttatgaaat
    72661 acagagagta taaaagcgtt ttagatagta taaaagaatc aaaagaaatt ttacaagaag
    72721 agtcagatga agaattaaga gaattagcta aaatggagtt agctgagatg gaagaaaaag
    72781 tagcacctct ggaggaagaa ataaaaatac tattattgcc taaagaccct aatgatgata
    72841 agaacgttat agttgaaatt agaggtggag ctggaggaga tgaagcagct ctatttgcgg
    72901 gtgatttatt tagaatgtac tcaaggtatg ctgaaagaag aagatggaaa atagaattat
    72961 tgagtgcaag tgatactgga gttggtggat ataaagaagt atcttttatg ataaaaggta
    73021 aaggtgctta ttcaagatta aagtatgaat ctggagttca tagagttcaa agaataccat
    73081 caactgaatc tggtggaaga atacacacat caacttctac tgttgcagta ttaccagaag
    73141 ttgaagatgt agaggttgaa ataaatccaa atgatttaag aatagatgtt ttccgttctt
    73201 cgggtaatgg aggacaaagt gtcaatacta ctgactctgc tgttagggta acacatatac
    73261 ctacaggtga agtagtatct tgtcaagatg gaaaatcaca attaaaaaat aaagagcaag
    73321 ctttaaaaat attaaaagct agactttacg ataaagcatt ggcagaacaa cacaaagata
    73381 tagcagcaga aagaaaaagc caagtcggaa caggagacag gtcagaaaga ataagaacat
    73441 ataatttccc tcaaggtaga ataagtgacc atagaataaa tttaacttta tataagttag
    73501 atgcattttt agatggagat atagatgaaa tgatagatgc tttaattact gttgaccaaa
    73561 cagaaaaaat gactgcaata taagtaatgt tttaaagaag ctgtttcttc ctagaaatta
    73621 ttttctattt tgaaacagct attttgcatt ttaggtcatg ttatcatatt ctattgtata
    73681 taattgttat atacaagtat aggagggtta tatgatacta aaagtaacaa taattgggct
    73741 tttggcaggt gttataggga caggattagg aggagtaata tcagccattt ttaaaagaga
    73801 ggtcgataaa tatctaagtt tttttatggg attatctggt ggaataatgt tggctgtagt
    73861 ggtgtttgat ttaatgaaag aatccatgga caaaatgggg ataatcaata cagttatatt
    73921 tacgtttgtt ggagctctca taactatgta cataaaaaca aaattagatg tgtctggaaa
    73981 tatggcatct ggatatctta tattcataag tatattacta cataatttac cagaaggact
    74041 agccattggt tcttctttta tgtcaactga aagtttaggt attacacttg ctatagttat
    74101 aggtctacat aatataccag aagggttagc tatggcatta ggcttagttt gcaataaaat
    74161 gaaactaagt aaagttattt tgtttactgt aatagctgga ttacctatgg gtctaggaag
    74221 ttttttaggt gtttattttg gaggagtatt tacttctctt ataggagtat ttcttgctac
    74281 agcaggaggt actatgatgt atgttgtatt ggaagaaatt tttccacatt caaaaagtgt
    74341 gtattgtata attggtttct tgttaggtac aatgattgta aattatatat aaaattaaaa
    74401 tgaaagcagg catacaaaag atgaaaacta ttataagtaa tattgatata aataatattg
    74461 attatgagga aataaaaata caagcaaaac ttcttagaga aggtaaaaca gttatttttc
    74521 caactgagac agtatatggg ttgggtgcaa atgcattaga tgaaaatgca gtaaagaaga
    74581 tttatgaagc taaaggaaga cctagtgaca atccattgat agtccatata tatgaaaaag
    74641 aagaagtata tgacttagcc aaagatataa gtgataaagc taaacttgtt atagaaaagc
    74701 tttggccagg acctataaca attatattaa ataaaaaaga tataattcca tacaaaacta
    74761 gtggagggct tgatacagtt gcaataagga tgccttctaa tgttattgcc agggcaataa
    74821 taaaagaagc tggtatacct attgcagctc cttctgcaaa tatctcaggt cgcccatctc
    74881 caacaaaagc aaaacatgta tatgaagaga tgaatggaag agtggatgga atagttttag
    74941 gaggagacag taattttgga ttggagtcaa ctgtattgga tttaacagaa gagactccta
    75001 tgatactgag accaggaagt ataactaaag aagttttaga ggaacttctt ggagaagtaa
    75061 agcttgaccc atcattaagt aaaaaagaag ataatcaaaa agctaaagct cctggaatga
    75121 aatataaaca ttattctcca aatgcagatg tatatataat atctggaaaa agagaaaatg
    75181 tagcaacaaa gattaatgat atgataaaat ctaatagaga caaaggtcta aagacaggtg
    75241 taatgtgtat cagtagaaac cgagagtttt atgatggaga agtaatcgat ttaggaaact
    75301 ctcttgaaga agttgcaagt aatctgtttg atgctcttat agaaatggac aaaaagggcg
    75361 ttgatgtcat atattcagaa gagttcccta agactggagt tggacaagca attatgaata
    75421 gactactgaa gtcagcagga tacaaagtga taaaggctta agagggagtt gtaaaatatg
    75481 aatatactta ttgtgtgtac aggtaatact tgtagaagcc ctatggctga ggctatacta
    75541 aggaaagcaa ttaaagaatc tggaagaagt attgaagaat attcgatatc atcagcaggt
    75601 atatctacag caaatggaat gggtgcttct gaaaattcta tagaagtact aaaagaaatc
    75661 ggtatagact tatcaaatca tagaagtaaa gtaattacta aaaaattaat tgatgaatca
    75721 gatataatac taacaatgac taaatctcat aaagaaattt tagtacaagc agttccaaaa
    75781 tgcaaagaaa aagtgtatac ttttaaaggg tttgcaaata aaaatgagga agatatatct
    75841 gacccatttg gtggaaattt ggatatttat agaagcacta tgagagaaat aatgtattct
    75901 gtcaacgaaa tagtaaaaaa aatatgaaac ggaggataaa caaatgaaga taggattagg
    75961 ctgtgaccat ggaggatata atttaaaaaa agaaataata agctatttag aaagtaaagg
    76021 tatagaatgt gtagattatg gaactaataa tgctacagat tctgttgatt atcctgttta
    76081 tggagaaata gttgcaaatt cagttataaa taaagaagta gattatggaa tattatgttg
    76141 tggtacaggg ataggaatat ctttagctgc taataaggtt cctgggataa gatgtgcagt
    76201 tgtatcagat gtattttctg caaaaatgtc aaaagcacat aatgatgcaa atatgttatc
    76261 attaggcgaa agagttttag gaaaaggtct agcacttgag atagttgaag catggattaa
    76321 tacggatttt gaaggtgata gacatgctag aagagtaaac atgattaaat caatagaaga
    76381 aaaacataat aaataataaa aattacagtt ttatcaggag gaattatcaa aatgagcaaa
    76441 gtggtagaga caaaccatcc attaatacaa cataaattaa ctttaatgag agataaaaat
    76501 actggttcta aagactttag agagctttta actgaaattg caatgttaat ggggtatgaa
    76561 ataacaaaag atataccatt aaaagatgta gaaatagaaa ctcctattca aaaaacatca
    76621 tctaaagtag ttgcaggtaa aaaattagca ataattccaa ttttaagagc aggtcttgga
    76681 atggtagatg gattagtaag cttaatgcca gcagcaaaag ttggacatgt aggactatat
    76741 agagacccag aaacattaaa accagttgaa tattattgca agcttcctca agacatagga
    76801 gaaagagata taatagtagt tgaccctatg cttgcaacag ggggttctgc tgtagcagct
    76861 atagatttac ttaagtctaa aggagctaaa agtataaaat tagctaattt agtagctgca
    76921 ccagaaggta tagcagaagt gcaaaaatat catgatgatg ttgatatata tgttgcatca
    76981 gttgacgaaa gattaaatga acatggatat ataattcctg gtcttggtga tgctggagat
    77041 agattgtttg gaactaaata aaaataaata actataaata tttattaggg actttcttat
    77101 atagtgtata taattttatg taagaaggtt cttttttata tattttttct caaaatagga
    77161 attatgaatt gataaatttc tcgaattgag aataaaaagt ttttaccaat taaaaaagat
    77221 tgcttatttt acaaggtttt atagaatagt agtctatttt aatgtggcat gttagttgct
    77281 tacgattaag attataggta ttaagtgtat tagttaaatt gagaattgaa tattaataat
    77341 attattggga gtggtaatac atgaagaatt gtatagcagt aagaggggct ggagatttag
    77401 caacagcagt tattcataaa ttacacaaca gtggttttaa agtagttgcg ttagaaatag
    77461 aagagccatt agctataaga aggcaaattt ctttttgtga ggctgtttat gaaaaaaaag
    77521 taactgttga aggtgtagaa tgtatatttt gcaatactga aaaagaaata gaagaagttt
    77581 tatctcaaca taaagtagcc ttgattatag acccaaaagg agaaaaatta tctaagttaa
    77641 atccaatcgt tttggttgat ggaatactcg ctaagaaaaa cttaggtaca actaaagata
    77701 tggcaccact tacaatagct ttaggtcctg gattttgtgc aggagaagat gtagatgttg
    77761 ttatagagac aatgagaggt cataatctag gaagaataat aactaatggt agagcagaaa
    77821 aaaatacagg agtaccagga gcaataaaag gtttttctaa agagagagtt atgcattcac
    77881 agtatgatgg tataatggaa aatgtatgta agataggtga tatagttgaa aagggagacc
    77941 tactttgtta tattaataat tatgaaaaaa aacatgaagt tagagcaaca atatctggag
    78001 tccttagagg tttattaaag gatggagcaa aggtgaacaa aaatttaaaa atactagaca
    78061 tagaccctag aaaagaagaa gttaagaatg cttttacaat atcagataaa gctagatgta
    78121 tagctggagg ggttttagaa gccatactaa acaatgggat ttatccccaa aattaattta
    78181 tattagataa agctattaat attttattaa gatgatataa taaatttaat agattaaaga
    78241 gagcatatga cgggtattta aaatttaaat ggtaaaaatg ctcttttttt attggacatt
    78301 caattaacat gtgtatataa tataaaaata agtatttatt taggttgaaa tctaataaat
    78361 atataatacc agtacataag caaagaggag aaaagtaaat tatgagacca tcatgggatg
    78421 aatacttcat ggaaattgca gaagtagtga aaaagcgttc aacttgtatt agaagacaag
    78481 taggagctgt aattgtaaga gacaaacaaa tattaacaac tggatataat ggttctccga
    78541 gaaatcttga acactgtgaa aatattggat gtaagaggca ggaattaaat attccctcag
    78601 gagaaaggca tgaattatgc agagcattac atgctgaaca aaatgcgata atacaagctg
    78661 ctcataatgg tattagtgta gatggggcaa ctttgtatgt tactacaaga ccttgtgttt
    78721 tgtgtgcaaa aatgtgcata aatgcaggta tattgaaaat tgtttatgaa ggtgattatc
    78781 cagatgatat gtctacagaa ttattaaaag aagcaggaat agaactcata aaattttagt
    78841 ggataaaatt tatatgacaa taaatgtcaa attatgacac tgaaatacaa tggtatcaaa
    78901 tgattaaaag aaagtatttg aaaaatcatc agattagtag taagatatat tttataatta
    78961 aataaattta tgagatagat taattacatg ttatttattt tataataatg agggatacta
    79021 tttatgagta aaagaaatac acatacagaa attgcaaagt tgtttagttt aataagtcaa
    79081 ataggattga tgattgtaat atcaatatta ggatgtactt tcttaggaaa atttttagat
    79141 agtaaattga atactactcc agttctgaca ataatatttt tattgttggg tgttggaggt
    79201 gcttttagtg gtgtttataa aactctaata gtatatacac gaaggaagtg atatattgga
    79261 tataaaatta cttaaccagg taaggtcagt aacaaaaggt atagtaatat ttgatgtagt
    79321 tttaattgtg ctaatgttga taacatcaac attaagtaaa ccagtattat taggacttat
    79381 atttggctct ataatagcta tgttaaattt tagattattg gcgatatcac tggaaaaatc
    79441 agtgacattt cctgctggga aggcacaagc ttatgcatca gcacaatatg ctattagatt
    79501 atttataatg gcagttgttc taattgcatc agctacagta ccacatttaa atataatcgg
    79561 aacagcattg ggattattga gcacaaagtt tgtcatatta tcaaaaaact ttataaataa
    79621 attaaagagg aaggaggcat aagtatgaat caagcgcatt attatctcat aggaaacttt
    79681 aagattagtg aaacaatact agtaagttgg ggaataatgg cctttttagc gatagcatct
    79741 tattttttga ctagaaactt aaaaaaagtt ccaactagta aagttcaaat attcttagaa
    79801 tttgcagtag gtggtcttgc taaaatggtt acagatgtta tgggtaaaaa tacagtaaaa
    79861 agaatgccat atatgatacc atacatagga agcttgtttt tatttttcgc atgttcaaat
    79921 ttaagcccat tacttggatt aaggtcacca acaaccgact tagacacaac tgctgcatgg
    79981 gcaatgatta cattttttat gatttatttt gcaggagtta aatttaaggg gttatcatac
    80041 tttaaagaat tggctcaacc acttgccata atgttaccaa tcaatattat aggtgagtta
    80101 gctagaccaa tatcattaag tttccgtcca tttgggaata ttttaggtgg aacaataata
    80161 atgggtctgt tttatcaact tttaggtttt atatctagtt taataccagg attatcaata
    80221 ccaattggag aaatattaat accagtccca ttacacttgt actttgactt atttgcagga
    80281 ttgttacaag catatatatt tataatgtta acaatggtat ttatatcaag tgcaggagag
    80341 ttagattaag aaatcaactt atattaagat aaaaataaaa taatttaaaa aattaaagtt
    80401 ttaaggagga aataattatg gaaacagcaa tagtagcggc agcatcagca ataggagcag
    80461 ggatagcagt tgcaacaggt ataggtgcag gtataggtca aggtatagca gcagctaaag
    80521 ctgcagaagc agtaggaaac caaccagaag ctaaaggtga tataacatca actttattac
    80581 taggagttgc tatagcagag tcttcagcaa tatatggttt ggttatatca ataatacttc
    80641 tattcgtaaa tccattcttt aaatacttag gaatgtagat ttagtcctta aatattttga
    80701 gataggttta taggtaaatc atctcaaact tataatcatg agatgaactt tatcatctcg
    80761 tgattataag tagttatgat ttatggaaag gaggataatt atggaaaaag cactagtagg
    80821 tataacgtgg gaatttgtgt tccaaattgt taatactttt ataatatttt tacttctgag
    80881 aaagctttta ttcaagccag tattaaatat aattgaatca agagaaaatg atattaaaag
    80941 tgatttggca gaaggcgaaa aagcaaaaaa tgaaggttta gctctgaaaa aagaatatga
    81001 gtcaaaaatc aattttgcca aggatgaagg tcaagaaatc ataaaacaag caactataag
    81061 agcagagcag aaatcagatg atatagtaaa tactgcaaag aaagatgctc ttgatataaa
    81121 agaaaaagca aacaaagata tagaacaaga aagacaaaag gttataaatg aaatcaaaaa
    81181 tgatatatct aacatagcac tacttgcagc atctaaagtt attgaaaaag atttagataa
    81241 atctaaacat gaagaattga tagaaaactt tataaaagag gtaggggaag ctaaatgata
    81301 aatgtaatag caaatagata tgccgaagcc ctatttcaat taggagaaga agaaaattca
    81361 actgatgtct tattcaaaga actagaaaaa gtagtagata tgatgactaa agttagtaaa
    81421 gatttctaca aagttttaaa atcaccatta gtttcaaaat cagaaaagaa aaacttggta
    81481 gaaattattt ttagtaagga agtaagttct aacataaaaa actttttaaa agttttagta
    81541 gataaagata gaatatcata tttagaagat atcgaattag cttataaaga attattaaat
    81601 aagaaaaaca atgtaattga tggagtagca ataagtgcta ttccaatgag tgaaacagat
    81661 attaaagaac ttgaagtcaa actttcaaat aaatacaaca aaaatgttac tatagaaaat
    81721 gttgttgaca agactatact agggggagtt ctagttagaa ttggaaatga acaaatagat
    81781 ggcactgtga aaactcgtct tgataagatg aaagaaaagc tatctgaagt aatatcttaa
    81841 gaatggcggt gaacccatga acttaaaacc tgaagaaata agttctataa ttaaacagca
    81901 aataaaaaat tatgagaata aagttgagtt gacagataca ggtagtgttt taacagttgg
    81961 ggatggtata gcgagtgtat atggattaga aaaagctatg tctggtgagt tgctagagtt
    82021 cccaggtgaa atatatggta tggcacttaa cttggaagaa gaagtagttg gagctgttat
    82081 ccttggagat gacagtgaaa taaaagaagg cgatatagtt aagagaactg gaagaatagt
    82141 tgaagttcca gttggagaag ctttaatagg tagagttgta aactcactag gtcagccaat
    82201 agatggaaaa ggacctatag cgtacacaaa aactagacca gttgaatctg aggcacctgg
    82261 tataatagat agaaggtctg tttatgaacc attacaaaca ggaatcaaat caatagattc
    82321 aatgatacca attggtagag gtcaaagaga gttaataata ggtgacagac aaactggtaa
    82381 aacatctata gttattgaca ctatactaaa tcaaaaagga aaagatgtta tctgtatata
    82441 tgtagcaatt ggacaaaaac gttctactat agctcaatta gttagctctt tagaaaaagg
    82501 tggagctttg gattatacaa tagtagtttc agcaacagca tctgaatcag caccacttca
    82561 atatatagca ccatatgctg gagcagctat gggtgaagaa tttatgtaca atgggaaaca
    82621 tgtacttata gtgtatgatg atttaagtaa acaagctgtt gcatatagag aaatgtcatt
    82681 gttacttaga agaccaccag gacgtgaggc atatcctgga gatgtattct atttacattc
    82741 aagattactt gaaagagcag ctaagttatc tgatgaattg ggtggaggtt caatgactgc
    82801 cttgcctata atagaaactc aagctggtga cgtttctgca tatataccaa caaatgttat
    82861 atctataaca gatggtcaaa tatacttaca accagagtta ttctattcag gtgtaagacc
    82921 agcagttgac cctggtatat cagtatcaag ggttggtggt tctgcgcaaa ttaaagcaat
    82981 gaaaaaagtt gcaggaacat taaaacttgc atattcacaa tatagagaac ttgcagcatt
    83041 ctcacagttt ggttctgact tagatgaaga tactaaaaag agacttgctc aaggtgaaag
    83101 aatcgttgag atattaaaac aaggtgagca tcaaccaata aaagttgaaa atcaagttat
    83161 gataatatat gctgttataa ataatcattt agaggatatt ccgatagata atatagctag
    83221 atttgaatca gaattgtatg catttgtaga taataattat ccggaaatat caagaaaaat
    83281 attaggtgga gaagacttca ctcatgattt aacagatgca ataaatgaat ttaaagaaaa
    83341 gtttgttgtt gaagtataat cctttttaaa aaaggaggta aattaattgg caggagctgg
    83401 aatcaaagca atcaaaacac gtatgggaac tgttgaaagt acaaagcaaa taactaaagc
    83461 aatggaatta gttgcatctt ctaagcttag aaaagcaaaa gaaaaggctg aagattcaag
    83521 agaatatttt aatactttat acaatacagt aaaagatatt gcccaaacaa cacgtggaat
    83581 tagaaatgaa tttttaaaag aaagagaagt aaaaaataaa tgttatgtag tagtagctgg
    83641 agacagagga cttgcaggtg gatacaattc caatgtatta aaggaatctg tctctcatat
    83701 gaaaggtaaa aaggaaacag tgattactgt tggaaagaaa gcacatgaat ttttttctaa
    83761 aagaaactac aatatagcaa aagatataaa tagtgttgaa gcttgtggat atgaagatgc
    83821 gacagaaatt tcacaaacta ttattgacct gtataaaaaa ggtgaagtag atgaagttta
    83881 tattgcttat acaaaattta aatctgcttt atcacaagaa gttgaaattt tgaaattgct
    83941 tccattagtc tttgaaaaag atacagacga acaaggaatg gcagtaaaag aagaaaaaca
    84001 aaatagatca aaagtacaat atataccatc agcagaagca gttttaagtt atattgtacc
    84061 tcagtttata tcaggtacag tatatggtgg aattttggaa tcttttgcat cagagcaagg
    84121 tgctagaaga actgcaatgg aatcagccac agacaatgcg aatgaaatgc tatctaattt
    84181 agagttaagt tataacagag caagacagtc agctgtaact caagaaataa cagagatagt
    84241 tggtggagtt gaagctctaa aatagggagg ttaggaaatg gcaaatgtag gtaaagtagt
    84301 acagattgta ggagctgtac tagatgttaa gtttgatagt gaacaaagcc tacctaactt
    84361 attaaatgcg ttagtaataa agttaggaga taaagaaata gttgcagaag ttgctcaaca
    84421 tataggtgat gatactgtaa gatgtatagc tatgagtgca acggacggac ttgtaagagg
    84481 tatggaagtt gtagatacag gagggccaat aagtgtccct gttggagatg agactttagg
    84541 tagaatattc aatgttcttg gaaaaccagt tgatgggaaa ccagctccaa aatctgcacc
    84601 taaattacct atacatagac cagcaccggc gtatgatgaa ctagaaacta cagcagagat
    84661 actagaaact ggtattaagg tagttgactt actagctcca tatctaaaag gtggaaaaat
    84721 aggtctattt ggaggagcag gagtaggaaa gacagttcta atacaagagc ttataaacaa
    84781 tattgctaag caacatggtg gtatttctgt attttcagga gtaggagaaa gaacaagaga
    84841 aggtaacgac ctttatggcg aaatgagtga gtctggagtt ataaataaaa cagctctagt
    84901 atttggtcaa atgaatgaac cacctggagc gagaatgaga gttgctttaa ctggacttac
    84961 aatggcagaa cattttagag atgagcaagg acaagacgtt ttacttttcg ttgataatat
    85021 attccgtttc acacaagctg gttcagaagt ttcagcactt ctaggacgta tgccatcagc
    85081 tgttggttat cagccaacat tagctactga aatgggtgca cttcaagaga gaataacatc
    85141 aactaagaaa ggttcaataa catctgttca agcagtatat gtaccagcag atgacttaac
    85201 tgacccagca ccagctacaa cattctctca cttagatgca aaaacagttt tatcaagaca
    85261 aatatcatca ctgggtatat atccagcagt tgaccctctt gaatctacat caaggattct
    85321 tgaccctagt atagtaggaa aagaacatta tgaagtagct agaggtgtac aatcaatact
    85381 tcaaagatat aaagaacttc aagatataat tgctatactt ggtatggatg aattatctga
    85441 tgaagataag ttaatagttg cacgtgctag aaagatacaa agattcttat ctcaatcatt
    85501 cacagttgct gagcaattta caggaaatcc aggtcaatat gttcctgtta aagagactgt
    85561 aagaggattt aaagaaattc ttgaagggaa acatgatgac ttaccagagt cagcattctt
    85621 atttgtagga acaatagaag acgctgttag aaaagcgaag ggaagtatgt agtcatggcc
    85681 agtgaatttt cattgaaagt tgtaactcca agtaatactt tttatgatgg agatgttgaa
    85741 atgatagtag ttagaactac aaaaggagac aaaggggtgt tgaaaggtca tataccattt
    85801 gtttcaggta tacctgtagg aactttaaag ataaaaaaag agggaagttt taaagaggcg
    85861 aaaatagcag aaggattcat taatgtaact caagataaaa ctgtcatatt gacagaatct
    85921 gctgagtgga tttaataaat taatttagag tataaaaagt cattttgacc aaaaaaactt
    85981 ttgggtaaaa tggctttttt gtgttttggt gttaataaaa taacgataaa attgtaagtt
    86041 tttagtgata ataaattcac aaaatgttta aatttactgc caaattatgt tgtaattaat
    86101 aagataaaga agatttagat agcttttatg taagtgaaaa agtcttcata taagaaatac
    86161 atataaaatt tttataaaat ttaaaaaatt tg
//
LOCUS       DAMJWP010000002        73913 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.002, whole genome shotgun sequence.
ACCESSION   DAMJWP010000002 DAMJWP010000000
VERSION     DAMJWP010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 73913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73913
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.002"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            225..863
                     /locus_tag="PC700_000087"
     CDS             225..863
                     /locus_tag="PC700_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2579422.1"
                     /translation="MSNQIKDFDITNDMFQESIPITTKTILEDMPSNDELNHVFSKGC
                     ERKMKKRKIVLITLLLIGVLLLGSILYNLFLVKAANISMLKESWNFDIPIPNKEIEVF
                     DTQDSINGDGQSYFIQGFSEKNFKKVFNLKGGIVVSKDNINEIEKYIDKFKRDSVNIN
                     KSNKNKIEEDFKKYKLEVKKDDKYIYKRNYENYVVLIIKKDEQKLYSLIWNQ"
     gene            complement(1341..3017)
                     /locus_tag="PC700_000088"
     CDS             complement(1341..3017)
                     /locus_tag="PC700_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF2579423.1"
                     /translation="MSKEKIKVYLYTRVSTSIQIDGYSLEAQKSRMKAFALYNDYEIV
                     GEYEDAGKSGKSIEGRVQFTRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIM
                     QDFGVNLICVEDGIDSSKDAGKLMISVLSAVAEIERENIRVQTMEGRIQKAKEGKWNG
                     GFAPYGYQLIDGKLIINEEEAIAIRTIFDQYVNTSIGANGLSKYLENHGIRKIPRQNG
                     KNPLFDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYRQVEQDDYLVAEGIHEAI
                     IPDELWQAAQVKLKAQAKKYEHVNKGKNMRTHLLSGIVKCPICGAGMFGNKSIKYKKD
                     GTKYKDFYYYGCKHRLMNRGHKCTYNKQIREELLDDAVAEVIIKLVSNPKFASMIQEK
                     INMKVDTSAIENEIDNYQNELRKSHSTKYKLIEEIDNLDVEDKHYKRRKNDLDDRLYR
                     MYDKIEELESQLIEAKAKKETIEVEKLTGDNIYKVLIYFDKLYKVMNDVERRQLIEAL
                     ISEIQIYEEKQPNGQWLKSITFKLPIIDEDLNISLENDEQVETVAKIIKQ"
     gene            complement(3118..3270)
                     /locus_tag="PC700_000089"
     CDS             complement(3118..3270)
                     /locus_tag="PC700_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2579424.1"
                     /translation="MDNDWNGLADLIANLITKYAGVLDLENLPNPTPVKNTEDKNKFD
                     IAKSTD"
     gene            complement(3759..4169)
                     /locus_tag="PC700_000090"
     CDS             complement(3759..4169)
                     /locus_tag="PC700_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004634151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2579425.1"
                     /translation="MAKEYYLYIRGQKVKVSEDIYIVYWREKEHEKYLEQVDRKNHLL
                     FFSALDHDGHFTDNIVDESVDVEKIVETQMMIEAVRSAITRLNAEERDIIERLYFNDE
                     TVRSVAKLKSITHPALIKRRNKILEKLKKFIEEL"
     gene            complement(4693..6012)
                     /locus_tag="PC700_000091"
     CDS             complement(4693..6012)
                     /locus_tag="PC700_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2579426.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLVFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLELYSKSSEIKAFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKTRLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKALQDEEFFIFKIKIFS"
     gene            complement(6009..6662)
                     /locus_tag="PC700_000092"
     CDS             complement(6009..6662)
                     /locus_tag="PC700_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2579427.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYNDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            complement(6760..8136)
                     /locus_tag="PC700_000093"
     CDS             complement(6760..8136)
                     /locus_tag="PC700_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2579428.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKIFQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSSKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDVHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFVGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIARQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFIIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(8136..8792)
                     /locus_tag="PC700_000094"
     CDS             complement(8136..8792)
                     /locus_tag="PC700_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2579429.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQKKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(8802..10079)
                     /locus_tag="PC700_000095"
     CDS             complement(8802..10079)
                     /locus_tag="PC700_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2579430.1"
                     /translation="MIKNAIAYITRKRNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIGENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNKSENNKIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKHIIKIMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMLNITTLGQ
                     SYLILISIIVLSVVFASSLILIKKPKEILSKIS"
     gene            complement(10098..10988)
                     /locus_tag="PC700_000096"
     CDS             complement(10098..10988)
                     /locus_tag="PC700_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2579431.1"
                     /translation="MDRIKNIINGSTKKDKYKIRDRFRHLFQSVWFLITNSYFEGFKT
                     GKIYGGNLKKICVPGMNCYSCPGAKGSCPIGSLQAVIGNSKYKISYYIVGILFFIGAL
                     VGRFVCGWLCPFGLIQDLLHKIPFFKKVETFKFDKHLRKLKYIILLVFVIILPLFLVD
                     ILGQGSPYFCKLICPIGMLEGGLPLVLLNKSMRNAIGFLYYWKGIILIVTLLLSIIIY
                     RPFCKYICPLGAIYSIFNPISIFRYRLDKDKCINCGKCKKVCQMNIDPVENCNHLECI
                     RCGRCKNACPVDAISYGVRK"
     gene            complement(10981..11076)
                     /locus_tag="PC700_000097"
     CDS             complement(10981..11076)
                     /locus_tag="PC700_000097"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDF2579432.1"
                     /translation="MAMIYYGHSNGETKVVLDKAIRICMECIGIG"
     gene            complement(11155..12312)
                     /locus_tag="PC700_000098"
     CDS             complement(11155..12312)
                     /locus_tag="PC700_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HDF2579433.1"
                     /translation="MKKIVSRGACLLLTLCLSLSTVGCKKSNESNKIKEGQSISSEEA
                     GMSTNEKDKNETFKPSDYTLKTKKEYVYEYLGLKFKLSDKFKKYMDDKKIAMLDDQSP
                     IDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELERIGTIGIFEKNTSEEKISK
                     ITKCDTHTKIGVSSDGKYDCYLSTNSGAESNLLDELKRTEIQIIDKKERPKNGFVLSE
                     KTDLENTEAFNKESVKDLRKLSTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKEI
                     PDLVEVQNEMKSKGVNIVGVVTDTVDDNGENKEAIEKSKLIHEKTKASYPFLMPDKTN
                     FNGRLNGIQAMPETFFVDSNGNIVGDTYSGAKSSKEWKQVIEKELEKIKNK"
     gene            12646..12996
                     /gene="mobC"
                     /locus_tag="PC700_000099"
     CDS             12646..12996
                     /gene="mobC"
                     /locus_tag="PC700_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HDF2579434.1"
                     /translation="MANRIRNERLEIKLTEEEKALFEEKRKLARCRNMSHFIRKCVLE
                     KEIYQVDLEPFRDLQGLLSNATSNINQIAKRVNSTGIIYKDDINDMKKQIEYFSKELW
                     QIHSLLLNRTSGGD"
     gene            13002..14333
                     /locus_tag="PC700_000100"
     CDS             13002..14333
                     /locus_tag="PC700_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDF2579435.1"
                     /translation="MAITKIHPIKSTLNLAISYIVNGKKTDEQILVSTHKCHQETAHT
                     QFLRTRNDVGTNGTVLARHLIQSFLPGEASPKMAHQIGLELCKKILKDEYEFVLSTHI
                     DKGHIHNHIIFNNVNMVTGKCYQSNKKSYHKIRYQSDKLCKENNLSVIDEFYESYKRK
                     YKTNGKSWYENEQSKRGTSWKSRLQFDIDRLIKQSKYWEEFLKKMAELGYEIKYGKHI
                     AFKPKDKTRFTRAKTIGEDYTEERLKERITENQSITTPPVKRRIGNIINMNANAKVKE
                     SKGYEYWATKHNLNTMAESVIFIREHGINSIKQLDKYIQKSAEERQDLQDKMKEIDKD
                     MQLLSDTMEQVHTVKKYRAYYKEYKANPSDKAFFEEHKAEITHYEIALSKLKKSYSKL
                     PNSKDILDKLNKLQEKKNTLIQEYSSTKSTMDELYQIRKNYGIYMGKEMER"
     gene            complement(14369..15175)
                     /locus_tag="PC700_000101"
     CDS             complement(14369..15175)
                     /locus_tag="PC700_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009354872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-like beta-propeller repeat protein"
                     /protein_id="HDF2579436.1"
                     /translation="MEISKKELRKISRKFRILASNVMNSYYGEQNANLEEMINYIEQT
                     LLIKEYLHSLEYDVDGLDDLLNTISNSYGRKVLNLGNDSNKRTYLLYRAFSFITGNNL
                     PTYTFGWYYAGSNKYQDMAKAFGDRMVYPFVLEVENYMKDISTDMGYDENNALYNINV
                     NSSGVQVNIAEHGSTINADQENKFNTEEVIKAINNVEDLIKGMEDDNSKSILNQNLDN
                     IKNEIKQKHPKKEVLTTCLKSMHFITTSVALIPDLALGIKMLASLIGISI"
     gene            complement(15165..15626)
                     /locus_tag="PC700_000102"
     CDS             complement(15165..15626)
                     /locus_tag="PC700_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HDF2579437.1"
                     /translation="MANDIKFEISVPADEEGYVQLKCPSCSEKFMLTVEDIEAESTID
                     IWCPNCGLTHENYLDDDIYELAERIVENQVAEMLNDFSKNLEKSFRNSKSIKFKRGKE
                     IKKEAELPIGRKTGDYEEKKYLCCGKTARISSIKKLEGSYCPFCGEMIDGD"
     gene            complement(15654..16721)
                     /locus_tag="PC700_000103"
     CDS             complement(15654..16721)
                     /locus_tag="PC700_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009354804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HDF2579438.1"
                     /translation="MSGKLYIAAAGSGKTRKIIEDSLNTHKKILILTYTITNEQQIIS
                     RIKNKVGVIPNNITVSTWFSFLLKDGIRPYQGSRFKERIEGIIFVNGQNNTYVKKTSN
                     KYFAKDNRVYTDKLSECVVELNDKCNGKVFERISGMYDCIYVDEVQDMVGYDLEILKC
                     LIASRIEVIMVGDPRQCTYSTHSSKKYKKYSCGKIDEFLKNECRKLDVRIDETTLNCT
                     YRNNDIICKFANSLYPERTSTNFVRRAEDESDGVMIIDKADVEKYLQKFNAMQLRENK
                     RTSVNNEYPYYNFGESKGLEFQRVLIYPTQPMWDWIINNNTSLKEQSKARLYVAVTRA
                     ENSVVIVRKDARPCSLPVVKL"
     gene            complement(16718..18340)
                     /locus_tag="PC700_000104"
     CDS             complement(16718..18340)
                     /locus_tag="PC700_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2579439.1"
                     /translation="MSIRKLKIKNFKCFSDWFIVDFENGINILVGNNGTGKSTILEAI
                     NLVLTGTYHGKNIRNELTQYLFNKNIVENYIREVNEGVNVEPPKIIIEAYFDAEMPSF
                     LGDGNSEKTKDTPGICLSISFDEKYSNEYKMLCKNKITSLPIEYYEVSWMSFARENIT
                     ARSIPIKSAMIDTTSYTPTSGSDIYISRIVKNLLDDNEIINISQAHRLMKEQFSRDEN
                     IANINRKLTETSNNELTISVDFGTKTSWESSLVTQLNEIPYNNIGKGTQCIVKTQLAL
                     SDHKIENKQIVLLEEPECHLSYSNLNILLDKVNESVKEKQIIVTTHSSFVSNKLGLEN
                     LILLNRGKTLRLGDLSNETNLFFKRIAGYDTLRLLLSNKAILVEGDSDELIVQRAYMD
                     SHNGKLPINDGIDIISVGISFLRFLEIAKLLELRVSVITDNDGNIDALNRKYSDYLGD
                     NCCENINIYFDDSTHEYNGSLENYNYNTLEPCIYRSNSMSILNEILNKSYEDPDDLLK
                     YMKGHKTEVALKIFESDKAIEFPQYIKEGILI"
     gene            complement(18543..18773)
                     /locus_tag="PC700_000105"
     CDS             complement(18543..18773)
                     /locus_tag="PC700_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2579440.1"
                     /translation="MRVFSYKRLFKKLIDLDMTNNELMEKAKVSKSTFYKMKNGQNVT
                     TDVLLRICNALDCDIEDIMECVIIEDVRRMGV"
     gene            complement(18913..19572)
                     /locus_tag="PC700_000106"
     CDS             complement(18913..19572)
                     /locus_tag="PC700_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006627214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF2579441.1"
                     /translation="MDYKTMRDRVEDMLSDNHKDFVKAIISMEKGINDESALDKLYDA
                     YMDNDSLNLLHEEFDYMIEDLRGQGQIKDLLYVREEKDNLINIVGNIVGEVDVIEREN
                     KNGETFKVANFSVVSKDDEGNKLYHNCSVYGEKSDIPKDFKQGDFVKLFGQIRTSIDD
                     NGKEHSNIRILSSKLLKAKEQMKGQEEKKESILGAIKKYQAEDKEKPKEKKEASKEAE
                     R"
     gene            complement(19615..28344)
                     /locus_tag="PC700_000107"
     CDS             complement(19615..28344)
                     /locus_tag="PC700_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDF2579442.1"
                     /translation="MRINDFHNILELIKQDVLYNEAEYLKLLKVVGNNQRYDFRSQLS
                     IYDKNPEATACAKFDYWRERFNRTVMRGQKGIPILEDYGTYKKVDYIFDISQTVSKNR
                     DVNEVNLWRFDKEAHQDVLKEMIKSEGYEESESTLENIFSLSRLYGDEKIDTLMNELR
                     VADEDRISFTKFVRDSVSYAVASRFKLDYPMDYELLRENFQRFDSISLMSLGESVSDI
                     SGKIIDETIQKSKELELQKEVLIGKEAGYNKIKEELEEVEENVFRRDDQERNENERVL
                     RNGEYGRDNRKNQGEYAKQLGGTDGLYKEVSESDLRSDEAELPFTERGAEPLRDAFGS
                     IQGEEADRTPDGYSETGSRVYESREAKVDSSLEDRGREQSAVWGDDFSSQRNDHQGSS
                     GDLKDNTDKGIIEAEKASFSLPENSYGQISLTIPLTEKDIDTVLINGGNHDGGRLPVI
                     AEFSKGKSIEELGEYLKDTFKGGNGFYIDEREVSSWYSDKGIHLAYGTSAREDNTQIL
                     SWSDAASRINELLDRGEFATNVELLEAQDYERDRISESLWYLTHDLSEEGIEQGYFNS
                     FERGGGFPQETKRLSEALKNPEYLKETIKEYSRFLEGYKESRNVLRYHYHKVDSLYQK
                     LQELELQRKEYSTNLTELPKVKLFITEDEVLESLSRGSGVDRGKERITKFFKENHTLQ
                     EKANFLKDEYGIGGHSHVVSGAMGSDEWHDAKGFKLQKNDCNDVFLTWTSVAKHIDEL
                     LSKKLYFEEKETESTAEIEEVKEPQYYSKNDPGNLMPDEILERVPEIYAQEDVDLADK
                     QVHAAYIIPFRSNWTWYMTEYDRESGDAFGLVLGIEPEWGYFNLEELKELNAQRLILE
                     DFPKTFRELKDIELIKQMDEQELQFVFNGELSFEDKVELEAPEETEEATKRGTVQATL
                     FDYLKEREEVELNEKEDSLLEEFAVKAGDTVYFNHEEYKVREISKNEITGRYDLWLDP
                     VRSGNHQIPIVDFTDNEDLLNKISLERPNFIVGDEVKYKDKNHTITRFDDMRKNLNTV
                     TVKDNTEYFGGMITSFDVIPYRLESDLERVFENLTYKQPEQTTEETQIRKAEAHNFKI
                     TEETLPEKLSPSERLNNNLEAISMLNRVESGQRELDSTAQEVLARYVGWGGLSEVFDE
                     SKEGQWKEARAFLNENLSHAEYEAARESTLTSFYTPKTVIDGIYKTLSDMGFKQGNIL
                     EPSMGIGNFIGNIPDEMNKSKFYGIELDSVSGRIGKLLYPESEVQIKGLEETSFSNNF
                     FDAVIGNIPFGEYKVNDREYNKNNFLIHDYFFAKSIDKVRNGGIIAFITSSGTMDKKD
                     ESVRRYLAARAEFLGAIRLPNDTFKGIAGTEVTSDIIFLKKRDSIRERDEDWIHLAED
                     ENGLTYNKYFVDHPEQVLGTMREVSGRFGNTLACLPKENADLKELLTKASEEISKGST
                     YEEIELLDDEITSIPATDDVKNFSYTVIDDEVYYRENSLFIKKEITDKNKEKIKDYLE
                     LNTALKDVIYKQKEDYSDDEVKKAQEKLNEVYDNFSKKHGFINNLSNTRALKEDSNFP
                     LVSSIEILDEEENVKAKGDIFSKRTITKAKVIDHVDTSLEALVLSVSEKGYVDFEYME
                     SLTGKDRPTLIEELRGEIYLNIREEQNFYRPLSFNLEDGDLPFACANGSNSYKYGYVT
                     KDEYLSGNIRDKIAIVDSYLAKLRQTERELPHLGFVENGKEKELISYEMNRLEYQKAE
                     LTKVLPKELEASEINVRLGATWIPIKDIEKFIFETLKTPGYARWDIKVKFSNITSEWN
                     VEGKSRDKGNDLAEMTFGTSRVNAYKLIEDALNLKETKVFDQIINPDGSKTSVLNKKE
                     TMLAGQKQELLKEEFKNWIFNDQERRNRLVKLYNERFNSIRNREYDGSNLSFEGMNTE
                     IDLRPHQRNAIARSLYGGNTLLAHVVGSGKTFEMVASAMESKRLGMCSKSLFVVPNHL
                     TGQIGREFMQLYPSANIMVADKKDFEPKNRKRFIGKIATGEYDAVVIGHTQFEKIPMS
                     KEYQEKHIQDQINEIVNYVEEYKHDRNQNFTVKQLEKTKKKLETRLEKLNDDFKKDDV
                     ITFEELGVDMLFVDEAHNYKNLYLYTKMRNVAGIGQSEAFKSSDMFMKCRYMDEMTGG
                     KGIVFATGTPVSNSMTELYTMQRYLQYESLKKNHLEHFDSWASTFGETQSSFELSPEG
                     TGYRVKTRFSKFYNLPELMSMFKEVADIQTADMLNLPTPEANYEVIKTEPSEEQKEIL
                     KSLSERADDVRNRVVEPDVDNMLKITNDGKKLALDQRLINPLLPDNPDSKVNVCVKNV
                     FAIWDKTKENRSTQLLFSDMSTPKGDGEFNIYDDIREKLVAMGIPKEEIAFIHEANSD
                     KQKDELFAKVRKGEVRILMGSTQKMGAGTNVQNKLIALHDLDVPWRPADLEQRAGRIV
                     RQGNENEKVNIYRYVTENTFDAYLWQTIENKQKFISQIMTSKTPVRVAEDVDESSLNY
                     AEIKALATGDPKIKEKMDLDNEVTKLKMLEANYKSNRYRLEDKVAKNYPEEIARTEKL
                     IEAVKKDISNVEPKAEGEEKFTSITIAGEKITDKKLAGERLLEAISKVKINESKVIGK
                     YRNMDLEVSYNFFTNSHNFSLNGVAKHLGELGTSADGNITRLDNALEKMPEKLNMLEE
                     KLISTKEQLENAKEELKKPFEKADELKTKVLRLAELNKLLDMGEVEEKKNDNPLVEDV
                     KRAIIDFCNREYEENHSYDEFHTLYPDLKHIGIAYTNTPDERHGIQYELNLEEKTWTQ
                     YIDDTPIKTESFDYENKGENEALRNMKNEIELSTFSDLVYVDSEDLRVATGLDIDDEG
                     NFYDPFAKDLDNDGIPDRYDSDFKDSDYFESTYDVEDNLHSKEETTQKSDDKPSILGQ
                     IRAYQEESKTEEKQKIKEQEFLR"
     gene            complement(28427..29230)
                     /locus_tag="PC700_000108"
     CDS             complement(28427..29230)
                     /locus_tag="PC700_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004804477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HDF2579443.1"
                     /translation="MSKINVEGSEISIIAIDNRDYISLTDMVRNIENGSALIEKWLRN
                     KNTIEFLGIWEEMYNTNFNSPEFEGIKNEAGFNRFILSVKQWVEKTNSIGIVARAGRY
                     GGTYAHKDIAFEFASWVSPYFKLYLIKEFERLKEEEQKKLGWDIKRNLAKINYRIHTD
                     AIKNKLVPDKLSKEKINFIYASEADILNVSLFGITAKEWRDENPDLKGNIRDYADISQ
                     LVCLSNLENLNAVFINEGMSQSERLEKLNAIAIEQMKILSESESIKKLK"
     gene            complement(29315..31021)
                     /locus_tag="PC700_000109"
     CDS             complement(29315..31021)
                     /locus_tag="PC700_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HDF2579444.1"
                     /translation="MKLVIAEKPSVAISIAKVIGANKKKDGYYEGNGYRVSWCVGHLI
                     QMANPDAYDEKYAKWNMADLPIIPSDYKYEVAKATKKQFNILKKLMNDKEVDTVINAC
                     DAGREGESIFRLVYNQVNCKKKMKRLWISSMEDSAIKEGFDNLTDGKDYDNLFESAQA
                     RAIADWLVGMNISRLYSCLYQQNYSVGRVQTPTLAMIVKRDDEIANYQKEKYYTVELS
                     MNGFTLSTDRIDDEVAAEQLISLVGDKIEITDVIQKEKITKPDLPFDLTTLQRECNKY
                     FGYSAKQTLDYTQSLYEKKLITYPRTDSRCLTEDMIVSTVNNILGKNDFDTERIKVVF
                     NSKKVTDHHAIIPTISSVKEDVSELPLSEAKVYFLISDKFHASVGYPLIENTTKIVAS
                     FDGFEFTSSGKVIKDEGFSKYLKEYKSKKSEDAVLPDVSVGDVLSVENKEVKEKFTQP
                     PKHFTEDTLLKSMEIAGNDALEKGVEVERKGLGTPATRAGIIENLIFKRFVERDKKNL
                     IATHKGISLVTIVADTFKSAETTAKWEMELSDIAQGKSSKKEFLDAIENEIKEAVLTY
                     RK"
     gene            complement(31116..32657)
                     /locus_tag="PC700_000110"
     CDS             complement(31116..32657)
                     /locus_tag="PC700_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HDF2579445.1"
                     /translation="MKTSLKKNNKFWTVALAVIVSISCILGLWTVVYAQEQKSEAPTK
                     NEAVQTEVEVNVRYIFEDEKVFKEEKIKAEKGQLIDSGDLPMLPDDMKFIDEFLFYEV
                     KGDGKDEIIRKVAKMEVKDKETQTEEEKPKQDECTQTEDKGTQTELSKDDISKIEKEA
                     KELQDKLDKLNCELKDKDKLSDKQKNKIKDLEDEIDSLKEKMKKDKGNKDLSEDMKKE
                     IDKLTEKVKELEKKATETNKAPVTSQSVTPISPISGIKTSSGISPQTPQTSVSSTGKG
                     SSDSVSSGNTTKDTGKGKTEVKEKEKEVRYPNKLTAKAPANNSQDSSMGSASKSVNTN
                     KGVASSPSKARASVTENKDNANKEYPIHHGDSSDNKETDQYSADARQFITFQTKNGKT
                     FHLIINHDEDSENVMLLTEVSEDDLLNMVEKKEAPKQEVVKEEPVKEEVKPEKKDEKS
                     NLGTYIILLLVVGGALGAGYYFKVVKKKEDKELEALEEEDDDFFSESESEEEINDADE
                     VEDEE"
     gene            complement(32632..32871)
                     /locus_tag="PC700_000111"
     CDS             complement(32632..32871)
                     /locus_tag="PC700_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2579446.1"
                     /translation="MKKELTTVKNRIKKLKDKKALIDEELEPLFIREEELENEEIIAI
                     CRKNNITISDLIAKVNRQKAEMKKEKTNENQFEKE"
     gene            complement(32883..35015)
                     /locus_tag="PC700_000112"
     CDS             complement(32883..35015)
                     /locus_tag="PC700_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDF2579447.1"
                     /translation="MGKKLKKDFKERHKASLEREMFHSKTDTIPEESKLKHNDDYRGK
                     IVHEKERFQDKVHEKTSKVSADNEKSYGTSKRNEKYRTSDKGDVNSAIETGRSDYITE
                     VKDGKIYDPLGKDLDNDGIIDRYDNDFRDSNYFESTYDVEDNLSHKEKSLENSFKNHK
                     TQKSRYKRKNYSESLYTRKKDDAPKGNKADDKKAGKDAVSGKVYSSLSKDQKKKLKKD
                     MVKVSALSGLAKGSETVRDYLSHGSDENQGVEAGEKTADTSSKLIHGIKKYSDKKRAK
                     RGYDLTNKDYKIRKRKSKLEFRDAKKELKKTDEYKRASAYKRFQKKNQVKAAISRENK
                     SRLRDRIKEGLIGTLKSSKDMIIRKAKGLMLIFIGIIILGTFVINFAGTGMTGFMNST
                     SSVLTTSYLSKPNVLSEINQKFSDMEGELQSEVDYVKENYPGYDEYILNNTEYIGHNV
                     HELLSYITSRCGEVKSVSEVESILKELFESMYDLEYREEIEIRYRTVTETYTDEDGNE
                     YTESHEEPYEYKKLIVTLHKKEMDSIIRKVFANYPDNLKHYEALFLAQGNMREAFGNS
                     DLISANGGIGGGKEYEASTEVQKKIVNAAYITPSPGAGWCAMWVSQVYQNAGLGYIGG
                     NACDMYRNYTFTSDRSKLKVGMLVAVESSSSGSSAGLTYGHVGIYIGDGKVIDNIGRI
                     RVTTLDDWIATFCKHHPVGFGFPPNVKK"
     gene            complement(35019..37448)
                     /locus_tag="PC700_000113"
     CDS             complement(35019..37448)
                     /locus_tag="PC700_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2579448.1"
                     /translation="MNKKRKIQQIKLMQNEKALRSQKRDLKKSNCKSKKDKGGILDLI
                     FKKEPKRYTVEDTIPYLRLLKSGICQLDEKHFSKSIAFQDINYQLALDEDRDLIFNQF
                     ANFLNSFDPSISIEFSYINQLGRNEEMKSAIQIPDKKDGFDDIRFEFREMLKSQIVKG
                     NNGLKKSKYVTFTVEADNLEQATSKLERLEIDILSSLKSMGVRAESLNGEERLKILHD
                     VLNPNKTFKFSYKDLKKRESTKTYIVPDEFNFTPSRYFKFGKFIGATSHFQILASELS
                     DRMLSEFLDIDDNINISFHIRAIDQSEAIKMVKRKNTDIDKMKIEENKKAVRSGYDMD
                     ILPSDLITYGEDVKSLLKDLQTRDERMFVVSIVFMNFARTVQKLDNTIAQISSIANKH
                     NCKLKRLDHSQEQGLVSVLPLGVNKIEIDRGLTSSSTAVFMPFTTEELFINSSNSLYY
                     GLNALSHNLIMADRKKLKNPNGLILGTPGSGKSFSAKREMANAILVTDDDVIICDPEG
                     EYGNLVRQFKGEVIKVSSKSKDYLNPLDINMNYGDGDAPLKDKANFIMSMLELVVGGS
                     GLTAEEKSVIDRCLPKIYEKYFEHPTPDNMPILQDLYDMLKGQEEKVGKKLATEMEIY
                     VSGSLNVFNHRSNVDLNKKLLCFDIKELGSQLKKIGMLVIQDQVWNKVSQNRGNKATR
                     YYIDEFHLLLKDEQTASYSVEIWKRFRKWGGIPTGITQNVKDLLMSKEIENIFDNTDF
                     VLMLNQASGDREILARKLKISLPQLRYVTNSNEGEGLLFFGNTIVPFIDKFPKDTILY
                     QKMTTKPEEVR"
     gene            complement(37360..37749)
                     /locus_tag="PC700_000114"
     CDS             complement(37360..37749)
                     /locus_tag="PC700_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HDF2579449.1"
                     /translation="MAYVPIPKDLNRVKTKVAFNLTKRQLIGFTLAGLVGIPVYLFMR
                     KFVPNDIAVIFLIVSTLPIFFITLFEKDGLTFEKYFKYIYLHKFYQPQKRVRKEVYLE
                     QEKKNSANKTHAKRKGIEKSKAGLKEK"
     gene            complement(37753..38013)
                     /locus_tag="PC700_000115"
     CDS             complement(37753..38013)
                     /locus_tag="PC700_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2579450.1"
                     /translation="MNKRKTVFIVVIGAGIMAIIDRIRLHNKVEELEEKTEDIGRCHN
                     DFYLIQQRYNKNTDEQLATIQEEIGSVYEHFEELSKGKEDGR"
     gene            complement(38031..38894)
                     /locus_tag="PC700_000116"
     CDS             complement(38031..38894)
                     /locus_tag="PC700_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2579451.1"
                     /translation="MFGIFDKIEEFFKELLLGGIQANLESMFLDINDKVGAVATDVGK
                     TPMGWNGDVFAFIKSINDSVIIPIAGLIITAVLCIELINMVMQKNNMHDTDTFEFFKY
                     IIKMWIAVWLVSHAFEFSMAVFDVAQHMVNKAAGVINTSAVISGDQIVKMVDGLKDKG
                     LGELVMILFETSLIKVAIQVISIVIMLVVYGRMFEIYVYSSISAIPFATMGNKEWGQI
                     GTNYIKGLFAIGLQGLFLMVCLGIYAVLVKTIQITDIHTSTFTILGYAVLLGLMMLKS
                     GTLAKSVLNAH"
     gene            complement(38914..39129)
                     /locus_tag="PC700_000117"
     CDS             complement(38914..39129)
                     /locus_tag="PC700_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002838961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family protein"
                     /protein_id="HDF2579452.1"
                     /translation="MEFFAQAVNVLKILVMAVGAGLGAWGVINLMEGYGNDNPGAKSQ
                     GIKQLMAGGGIVLIGLKLIPLLANVLK"
     gene            complement(39133..39444)
                     /locus_tag="PC700_000118"
     CDS             complement(39133..39444)
                     /locus_tag="PC700_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF2579453.1"
                     /translation="MKQEMININANLVAEPTFSSFEKEGEEVEVVNFTLVKKYGKGKE
                     YINCAAYGEKAEKAEDFEKGDLIHVFGYFKKREKEGKTYKNFVVKSYNKIEKKEENEE
                     E"
     gene            complement(39608..39841)
                     /locus_tag="PC700_000119"
                     /pseudo
     CDS             complement(39608..39841)
                     /locus_tag="PC700_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG"
     gene            39983..40156
                     /locus_tag="PC700_000120"
     CDS             39983..40156
                     /locus_tag="PC700_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2579454.1"
                     /translation="MDYMTLKEASEKWGVSSRQINYYCTDGRIPGAVKMAGVWLIPKE
                     AEKPKDRRYNESR"
     gene            40161..40871
                     /locus_tag="PC700_000121"
     CDS             40161..40871
                     /locus_tag="PC700_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2579455.1"
                     /translation="MLHIAICDDEKDFVQYLTDLLNQYSKETGRDIKITPYYDGMELI
                     EKYDPTIDLIFLDIQMKVVDGLHAAERIRQMDETVGIIFLTTLTQYGLEGYKYHATNY
                     IIKPMKYARLKSEMDKFLERSQKEDTPSLVITNDTGKYKVPLKSIRYVETYNRNLMFH
                     TEQENIICYKSMKEVERELCDKDFVRCHTSYIVNLFYVKGIKKLDIELITGEIIPISQ
                     PKRKEFMERLTDYWGDML"
     gene            complement(41142..41303)
                     /locus_tag="PC700_000122"
                     /pseudo
     CDS             complement(41142..41303)
                     /locus_tag="PC700_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569186.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            41914..42099
                     /locus_tag="PC700_000123"
     CDS             41914..42099
                     /locus_tag="PC700_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2579456.1"
                     /translation="MLKKYWIKCPICNGKTRVQVFHNTVLKDFPLFCPKCKLTHIIDV
                     EKLEIVIKNTEKQTFII"
     gene            42116..43099
                     /locus_tag="PC700_000124"
     CDS             42116..43099
                     /locus_tag="PC700_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004607971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDF2579457.1"
                     /translation="MKRLPKYTPAEVRNDPYGFTYKEMSEVIGENEAKALYEELYKQL
                     PRKKNLSMLVKNICKSSDTEKYVYELKDNKYIETVFIKRRDGGTVCVSTQVGCPVGCI
                     FCESGRNGFVRNLTSSEIVQQIILLRRKVNRIVFMGMGEPLFNYDNLIKAIHILRDRY
                     GLNFPTDGITISTVGPVDQLKKLREEHLKIQLTISLHAATQSARNRIIPHMRIYAIED
                     VVKQALSYSERHNRKIVFAYLLLPGINDRPSDVRQLAKWFRGKKVMINVLQYNPTSNS
                     RIKAPQKREIVAFKHQLEQAGLEVTMRVSHGREINAACGQLANTYNKFKKK"
     gene            43573..44010
                     /locus_tag="PC700_000125"
     CDS             43573..44010
                     /locus_tag="PC700_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003508282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2579458.1"
                     /translation="MELSSSDKERIQHQYDALAKKTLVGEAKSHRRTLAKRAAREVTF
                     SDLSESELAQLFTTDEYESDYFRFQVSGFDVLVKNELLAEALNALPERKRDIILLSYF
                     LDMSDAEIGELLNVVRTTVFRHRKSALAKIKQYLEGKADDEYR"
     gene            43994..44236
                     /locus_tag="PC700_000126"
     CDS             43994..44236
                     /locus_tag="PC700_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2579459.1"
                     /translation="MMNTVRKSENLLPFPVISAAANGDTTAMCAILKHYEGYIAKLCT
                     RTLKDDAGNTYSYVDEEMRNRLQVRLITRTLAFHVG"
     gene            44695..44868
                     /locus_tag="PC700_000127"
     CDS             44695..44868
                     /locus_tag="PC700_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-encoded TnpW family protein"
                     /protein_id="HDF2579460.1"
                     /translation="MDKKQTITTERKIGKITYLVQALPSEKATDTIHKKIEKLIVKDL
                     QKKPGNPGFLASE"
     gene            44946..46619
                     /locus_tag="PC700_000128"
     CDS             44946..46619
                     /locus_tag="PC700_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016289898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF2579461.1"
                     /translation="MLQSNKITALYCRLSQEDMQAGESGSIQHQKMILQRYADEHHFL
                     NTKFFVDDGFSGVSFEREGLQAMLQEVEAGRVATVITKDLSRLGRNYLKTGELIEIVF
                     PENGVRYIAINDGVDTAREDNEFTPLRNWFNEFYARDTSKKIRAVKQAQAQKGERVNG
                     EYPYGYIPDPNNRHHLIPDPETAPIVKQVFAMFVSGVRMCEIQKWLAENKVLTIGALR
                     YQRTGQARYQRAMIAPYTWPDKTLYDILARQEYLGHTITAKTHKVSYKSKKTRKNEEE
                     QRYFFPNTHEPLVDEETFELAQKRIATRHRPTKAAEIDIFSGLLFCAGCRHKMYYQQG
                     VNIEPRKFSYSCGAWRNRARTGSECTSHYIRKNVLLDLVLEDMRRVLRYVKEHEQDFI
                     CKATEYGDMEARKALAQQQKELFKAQARMTELDTLFRKLYEDNALGRLTDERFVFLTS
                     GYVDEKKSLAARIDELQQQIATVTERKRDISRFIQIVGKYSDIQELTYENVHEFIDRI
                     LIHELDRETNTRKIEIHYSFVGQVDTEQEPTQVVNHDRRNMVDVKSIAI"
     gene            46643..47098
                     /locus_tag="PC700_000129"
     CDS             46643..47098
                     /locus_tag="PC700_000129"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2579462.1"
                     /translation="MKEYTLIIKGEMDFIILSPQVLSSLITQIHNSPERKVVVSIESI
                     MPPKFTDYLLRVINSNRFSNERFRYRYILENPVTKKGMYEILRQQLSRTNTERFPCFQ
                     TIQLTDTFQGNVELDMECNDLFFWACKDTAAKFVYTFPDGREETLVIEY"
     gene            complement(47146..48798)
                     /locus_tag="PC700_000130"
     CDS             complement(47146..48798)
                     /locus_tag="PC700_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008978103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF2579463.1"
                     /translation="MSRKKQVYEKITALYCRISLDDGGDNESMSISNQKLMLRDFAEK
                     HGMFQYEYYVDDGYTGRNFNRPSFQRMIADIEAGKIGCVITKDLSRLGRNYIEAGSYI
                     EIFFPKHNVRYIAVTDGVDSLTRQEMDITPFKNILNDMYSRDISKKVLAGRMTRSRQG
                     KFCGGQPPLGLMRDPEDKGHLILDPETAPVIRKIYDMALDGWGCMRIAKQLMDDKIPI
                     TRVKSNTECDVNYYSWGSARISHILRNPFYKGAHLVCRTHQKGIRSNTYDIIPREDWE
                     VIEGCHEAIVTPEEWEQVQDIIDRRPTIMKGNACPFYNLFHGLVYCATCGKSMQVRYE
                     KVGRTGKNRFTGEMREPIDRAYYICQTYNRLGKNACTSHKIEARDLYNLVLKDIQELA
                     AQAMKDADAFYQRLSSRMERRYLVDASQTEKERKRLEDRNQEIDGMFLSLYTDKAKGV
                     LTEQRFMKLTAALEQEQEANQKRLHDLAVMQSRADAQESEVRTFIKEIRRYAAIAELD
                     ESVLNRLISKILIGEVKKVDGQKVQEVRIVYNFVGEIPEIAA"
     gene            complement(48871..49038)
                     /locus_tag="PC700_000131"
     CDS             complement(48871..49038)
                     /locus_tag="PC700_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008978104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2579464.1"
                     /translation="MEKSKNQKNKVRFVVVREFSGEKSMREAFEQLIERQTCEHFEEW
                     RHQREMAEKAA"
     gene            complement(49306..50664)
                     /locus_tag="PC700_000132"
     CDS             complement(49306..50664)
                     /locus_tag="PC700_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence-associated protein E"
                     /protein_id="HDF2579465.1"
                     /translation="MSAAEALAPEQSVDEVREGLSVTEKGQPANTIGNCRTVFSHDPL
                     LRDAIRLNLLTDRVDIVRDLGWRRNTSALTDTDVKYLLLYFEQNYGLTSEKKMTAALS
                     IVANENCYHPIQDVLNGLVWDGTPRIRSCLHHFLGAEVSDYVEEMLKHFLLGAIRRVF
                     SPGSKYEEMLCLVGGQGAGKSSFFRLLAIRDEWFSDDLKKLDDDRVFLKLQGHWIIEM
                     SEMLATSSAKSIEEIRSFISRQKETYRTPYEAQPKDRLRQCVFGGSSNTLDFLPLDRA
                     GNRRFLPIMIYPEKAEVHILEDEDASRAYLLQVWAEAMTIYRSGKYSMKFSKSIQRQL
                     VEVQKDFMPEDTEAGQIQGFLEHYTGSMVCSKQLFKEALGHTYDEPKRWQLHNINEIM
                     NTVVTGWKPFSNPRMFAGYGRQKGWERDVSGNELPGNEDGFVELSEEECRQLELPKEW
                     IA"
     gene            complement(50624..51310)
                     /locus_tag="PC700_000133"
     CDS             complement(50624..51310)
                     /locus_tag="PC700_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDF2579466.1"
                     /translation="MKTGCLFLCLEVRKSNVFEAVKQAVPTRQAAESYGIQLGRNGMT
                     CCPFHDDKHPSMKVDRRFHCFGCQADGDVIDFTARLFGLNKKDAALKLAEDFSVGFDA
                     KGHDPPRRKPGKRKISEELRYRQAEQKCFRVLCDYLHLLERWEKEYAPQTPEEAWNPL
                     FVEALQKKPYTEYLLDILLSGSMEERACVVAEYGKEVRKIEQRISEFTASHPAGRHER
                     SRSFSAGAER"
     gene            complement(51326..52570)
                     /locus_tag="PC700_000134"
     CDS             complement(51326..52570)
                     /locus_tag="PC700_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/arginine repetitive matrix protein 2"
                     /protein_id="HDF2579467.1"
                     /translation="MKLTRHNGRSGKHGTYNPRHNDRRFDVENSEHIDAERARQNVYW
                     DCYRGFTTHEFRENPEQPDFSFEEIERMYYNEHYGGHVEAQNARNEKTRHTERNRTVE
                     DLLKNNKTCPEESIYQIGTMGESVPPDTLFSIVNEFYEEFERRFGSHIHILDWALHLD
                     EGTPHIHERHVFDCENRYGELCPQQEKALEELGIPLPNPEKPKGRNNNRKQTFDAVCR
                     TILFDIARRHGLHLDQEPSYGGRDYLEKQDYILMKQKEQLAAQEQKLEELTLKIEDVE
                     TLLDDVSDAAYDKAVEVVTDTVRQETHKEDIRLVEESKKWVLSPERKAPKKEREYAAE
                     RLDGVITKIKNAMQHALAKIQRTLMQPEVKQAGKEQVKKKAKESIMDILAKAKINADR
                     DNRERWEREGRIAPTKKNDIEL"
     gene            complement(52998..53303)
                     /locus_tag="PC700_000135"
     CDS             complement(52998..53303)
                     /locus_tag="PC700_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDF2579468.1"
                     /translation="MRKLSKYEKETIINWNEAENLASVYTFNASLKRRLADFSRKYPL
                     LCRLERSTPEGSVTYVLDKSRLSIRLVPPYSEERKAAASAYAKEHGFQVVGAEEKIA"
     gene            complement(53287..53580)
                     /locus_tag="PC700_000136"
     CDS             complement(53287..53580)
                     /locus_tag="PC700_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2579469.1"
                     /translation="MTLSMKEKKILYAYGCPSHHNTVTRLKWLTALTVDPEAKRRMLG
                     LARKVETEVGENWYEDFYHHLRMEMDEYRRLKRSLRVLKSYTDYEEDLYEEAV"
     gene            53946..54794
                     /locus_tag="PC700_000137"
     CDS             53946..54794
                     /locus_tag="PC700_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2579470.1"
                     /translation="MYAKLTIPERLKDLRVVDKHLTLEQLAEQTGLSKSALGKYETDD
                     YKDISPFAIATLADFYGVSTDYLMGLTENKNHPNIELQSLHLSDDMVELLSSGKINNR
                     LLCELATHPNFRRLMIDMEICIDQIANMRVEQMNLVLEATRQTVMNKYAPGEDDLYMR
                     TLELGQVQESDFYSHVMHDDLDSIVRDIREAHRKDKTTADPQPTLDDVKEKFEQAIGQ
                     GSDTEMLIHEFCDKLQIPFEKISSEDFSAFLRILSLSKQLKSSNNMRGKASPIPPNRK
                     ARRKRK"
     gene            complement(54916..55197)
                     /locus_tag="PC700_000138"
     CDS             complement(54916..55197)
                     /locus_tag="PC700_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015574404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2579471.1"
                     /translation="MILEEMYNGRFYPCETVVADSPRFKQAVKASAALMDTLSERLSK
                     EDYALVEELREQVAIAQCEENESHFKYGFSAGLLVQQEAHAQVSQKDKE"
     gene            complement(55211..55588)
                     /locus_tag="PC700_000139"
     CDS             complement(55211..55588)
                     /locus_tag="PC700_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnpV protein"
                     /protein_id="HDF2579472.1"
                     /translation="MEHLPKKIHHNGISYTLVGDYYIPDLKLSEEIRPIGRWGRIHKA
                     FLQEHRPGQYNELLLSGKLWTYLADLNEQADDRLACIISQMQAAEGVTEELKARDQLA
                     WVGGMNSIRSRVEEIILSEMIFV"
     gene            complement(55686..57248)
                     /locus_tag="PC700_000140"
                     /pseudo
     CDS             complement(55686..57248)
                     /locus_tag="PC700_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565768.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
     gene            complement(57245..57730)
                     /locus_tag="PC700_000141"
     CDS             complement(57245..57730)
                     /locus_tag="PC700_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HDF2579473.1"
                     /translation="MINEEIARKTLNMEINAGKVTGKVLLNLLKKLMKEAEKFGGLEK
                     LVNANGNEVKLKDMVKKGQLEEIPVEEAELKELKKELNRYGVKFSVMKNKETGKNSVF
                     FQAKDMKVMDKAFKHALSKSEKKAERKESIHKNIEKFKEMAKNSISKEKIKNKQKEQS
                     L"
     gene            complement(57732..58754)
                     /locus_tag="PC700_000142"
     CDS             complement(57732..58754)
                     /locus_tag="PC700_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2579474.1"
                     /translation="MYLFDEQPIVANKTLAREIGLNEALILQQINYWIEINKKSGKNY
                     HDGKYWTYNSIRAWQENDFDYMSFDTVKRTFSKLEKAGYLLVGNYNKDPRDKTKWYTI
                     NNDKLEELYMDMDLKKQKIQTEALKNSMPNALGQNAPMDKGKISQCNNADCTNAQVQN
                     ALMQKDNLHQPLPENTTNNTTNNTTNNSAYISIHPSIKEKNQLFSENMKGGRIDISYK
                     NIYQNCLEELRHQTGYYEHMSIGNKSVAEEFDEILKILADVMVLDDESFITINQTKIS
                     VQVVKERFRKLNSGHLEYLVGVLRENEYRIRNIRAFVLTAAYNAPSGIDAYYSALVSY
                     DMRGGY"
     gene            complement(58840..59121)
                     /locus_tag="PC700_000143"
     CDS             complement(58840..59121)
                     /locus_tag="PC700_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2579475.1"
                     /translation="MRWILKIILFPISIALSILIAFLSFLLGIGTAILYLLMMFCIFG
                     AIASFLQKEVTIGIEALILGFLLSPYGIPMVGTAVIAVLTGVKEAIRSI"
     gene            complement(59140..60774)
                     /gene="rlmD"
                     /locus_tag="PC700_000144"
     CDS             complement(59140..60774)
                     /gene="rlmD"
                     /locus_tag="PC700_000144"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDF2579476.1"
                     /translation="MLSKNEMYIVDIVDIGQGGVGVGKYNGITVFVEGGLIHDKVKVR
                     INKVKKNYAEGDIVEIIEKSPFRVKRVCSDNLRDCGGCQIQDLDYNKQLEMKTNEVKQ
                     VLSRIGKLEDVEVHDTIGMDNPYRYRNKAQFPIQKNNGIPIIGFYKKKTHDVISTEKC
                     VIQHEINDKIVKIIKTYIRAYKVSIYDEKTHTGLIRHLVTKIGFTTKEVMVVLVANGN
                     KLPYLKELASVLKENIPGFKTLVVNVNQKDTNAILGRENIVIYGDGKINDYIGDLVFE
                     ISPLSFFQVNPVQTKVLYDKALEYANLNDTDTVFDIYCGIGTISLFLAQKAKKVYGIE
                     IIDDAIKDAKINAKLNNIHNAEFYVGKAEEIVPKMYREGKTANVVVVDPPRKGCDEKV
                     LETIVSMNPERIVYVSCNPSTLARDLDYLNVHGFKCVEAQPVDMFPHSTHVETVALLS
                     KLDVDKHIDVEIKLDELDLTSAESKATYAQIKEYVLEKFGLKVSTLYIAQIKKKCGIE
                     LREHYNKSKKDNQAIPQCTPEKEEAIMDALRHFKMI"
     gene            complement(60949..62709)
                     /gene="pyk"
                     /locus_tag="PC700_000145"
     CDS             complement(60949..62709)
                     /gene="pyk"
                     /locus_tag="PC700_000145"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDF2579477.1"
                     /translation="MLNNVKKTKIVCTLGPASQSEEILTQLMQNGLNVCRFNFSHGSH
                     EEHKERIDMAKKVREKLNKPVAILLDTKGPEIRTGNFEDPEVFLEEGQKFTITMKDIM
                     GTKEMCTVSYKGLAEDVKSGDSILIDDGLVGLRVKEINGEDIVCVVENSGIVKNHKGV
                     NVPGVKINLPAITPKDISDIEFGISQGIDYIAASFVRKASDVLAIREVLENNNATDIQ
                     IISKIENQEGVENLDEILKVSDGIMVARGDLGVEIPTEEMPIVQKMMIKKCNELAKPV
                     VTATQMLDSMIRNPRPTRAEVTDVANAIYDGTDAIMLSGETAAGKYPVEAVKMMATIA
                     KRTEETLDYDRLLKENGTNNVTVTDAISHATCTTAVDLNASAIITSTSSGYTARMVSK
                     FRPKSPIIATTNNEKTMNKLALTWGVYPVKSSVAGNTDEVIEKAIEAARQADYIDNGE
                     LVVITAGVPVGVSGTTNLIKVHVISEEIVQGIGVGTQTVEGKVRIIKNGNACVDFNEG
                     DILVSTATDAEMNNYIEKAGAVVTENGGMTSHTAIVGINLDIPVIVSATDITNLVKDG
                     EVVTVDASRGVVYRGSTRVL"
     gene            complement(62761..63720)
                     /gene="pfkA"
                     /locus_tag="PC700_000146"
     CDS             complement(62761..63720)
                     /gene="pfkA"
                     /locus_tag="PC700_000146"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDF2579478.1"
                     /translation="MKTIGLLTSGGDAPGMNAAIRAVVRSAIYYGCKVYGINRGYKGL
                     LEEDLTEMNLSSVGDIIHRGGTILKSSRCEEFKTEEGRLKAVKILKKYKIDCLVVIGG
                     DGSFAGAQKLSDLGFPAIGIPGTIDNDLAYTDYTIGFDTAMNTIIDAIGKIRDTSSSH
                     ERVNIVEVMGRHCGDLALYAGLAGGAETIIVPEVEITVDEVALRLKTTQKRGKRHSII
                     VLAEGVGSASDLEKELKKESGADLRVTVLGHVQRGGSPTVSDRILASRLGVRAVELLL
                     DGKSARVVGIKENKIIDLEISEALAQKKVFDKEAYEMAKILSI"
     gene            complement(63929..67498)
                     /locus_tag="PC700_000147"
     CDS             complement(63929..67498)
                     /locus_tag="PC700_000147"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDF2579479.1"
                     /translation="MNKDFAHLHVHTEYSLLDGFSRVKKLIKRAKELNMSSIAITDHG
                     CMFGVIDFYKTAIKEGIKPIIGCEVYTAARGLRDKDPNYDKYQGHLVLLAKNMEGYKN
                     LIKIVSTSYVEGFYYKPRVDMEELKKHNKGIIALSACLAGDVARALMNRNYEKAKQFA
                     LDYRDIFGEENFFLEIQDHNLPEQKEVNSGLVKLSKETGIPLVATNDVHYVNKEDSKI
                     HDVLMCIQMGKTLNDPNRMRFGSDEFYLKSREEMEELFPYALEAIDNTVKIADMCNVE
                     FDFNTIHLPKYDVPEGYTPETYLRELCFNGLKERYDNPSNEILERLNYELDVIEKMGY
                     VEYFLIVWDFINFSKENNIIVGPGRGSAAGSIVAYTLKITDIDPIKYSLLFERFLNPE
                     RISMPDIDIDFCYERREEVIDYVKRKYGDDHVAQIITFGTMGAKAAIRDVGRVLDIGY
                     NKVDKVAKEIPFALGMTIDKALDTNPNLKELYDQDPETKEIINISKQIEGMLRHASTH
                     AAGVVISKNPVDEYVPLYKHQDAITTQFTMTTLEELGLLKMDFLGLRTLTVIRDTLDL
                     IEKNRVIKNYTEKIDFSKMDYDDPKVYEMLSSGNTLGVFQLESAGMRSFMKQLKPDNF
                     EDIVAGISLFRPGPMDSIPAYIKNKSNPKDVTYLHEKLKPIMEVTYGCLVYQEQVMQV
                     VRDLGGYSYGRSDLVRRAMSKKKMDVMEEERQYFIHGKFDEDGNIEIAGCIRNGVEEE
                     IANKIFDDMIDFARYAFNKSHAAAYGVLAYETAYLKTYYPVEFMAALITSVMGNTDKV
                     VEYIRECKALEIDVLPPDINKSFSKFSVEGDNIRFGLAAVKNVGVNIINNIIAERESN
                     GLFVDLVDLVKRLDQKDTNKRVIESLIKCGAFDNISENRASLMAGYESLLESVSMDRK
                     KNVQGQISLFDAFGDSEEDSDFQQIYSLPKRQEYEERERLNLEKEVLGMYVSGHPLSQ
                     FEKELQEKTSMDNGKLNSLKEEEESYLQMNETDVIMGGMIVTKTIKTTKRNEIMAFIE
                     LEDLYGAIEIIVFPQLLQKYNVILNEDNIIYVKGTLSIKEGENAKLIAREIKDINDDT
                     DFADRNYNSGRTRNVENIKPESGKKLYLKIDSMSDTDTTNNIVEIAKQYPGNDSIYLY
                     PMDENINGKRKAYKMTGALTCICDEFINNLEELLPKDNIKVKA"
     gene            complement(67655..68602)
                     /gene="whiA"
                     /locus_tag="PC700_000148"
     CDS             complement(67655..68602)
                     /gene="whiA"
                     /locus_tag="PC700_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HDF2579480.1"
                     /translation="MSFSTETKNELARVISGNECCNIAELSALVKSGGSIQIVGYKKL
                     NLKITTELNSIARKVFKLLKKNFNINTTISVNKNQMLKRNNSYVLMVTSEMGSEMLLK
                     KLGILEDKEGFFTINKISENLIKHEGCKRAFIRGAFLGGGSISDPEKNYHMEFVTNNE
                     EFADSLKELINSLGFNSKIVARKNNHVVYIKESEQISDLLSIIGGHHALLSLQNTKIV
                     KEMRNNVNRIVNCETANLSKTVNAAVRQVENIKLIQETIGISSLPENLQEIAKIRIEY
                     EDMTLKELGEMLEPPIGKSGVNHRLRKIEEIATDLKKKS"
     gene            complement(68619..69044)
                     /locus_tag="PC700_000149"
     CDS             complement(68619..69044)
                     /locus_tag="PC700_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF2579481.1"
                     /translation="MREEVSAGGVVLFGNTILLLRKFNGDWVLPKGKVEEGENNQEAA
                     LREVSEETGVKADILKYLGEIHYTFKENWDENRAVHKTVFWYLMQAKNMDTIPQKEEG
                     FIDAKFIHLDRVVDLARYDDEKEIIKVALQEIKKRLKKN"
     gene            complement(69113..70219)
                     /locus_tag="PC700_000150"
     CDS             complement(69113..70219)
                     /locus_tag="PC700_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HDF2579482.1"
                     /translation="MAKLLIIVGILGWIALIVSLFIYKKAKNEKVKLFQRSIVENREP
                     NVVVIGGGTGQSVFLRGLKHTTQNITAIVTVADDGGGSGVLREDLGMLPPGDIRNCLL
                     ALANIEPTMNEVMQYRFTEGLLKGQSFGNLFLAAMNGLYGNFEKAVYKLSEIFAITGR
                     VLPVTLEDVNLVAKLENGNIINGESSIPEESKIQKSSIDKIFLNPKDVKPLKDVIASI
                     YDADIIIMGPGSLYTSIIPNLLVEGIVDAIKSSVAPKVYIANIMTQPGETEGYNVLEH
                     VNAIVKHTDENLIDYVIANNEILPEEMLDLYKQDGAEQVLLDKKQKDKLKEMGIKTVE
                     KNLIEIKNNYIRHDAKYISNIVIELALNHNYNKS"
     gene            complement(70231..71088)
                     /gene="rapZ"
                     /locus_tag="PC700_000151"
     CDS             complement(70231..71088)
                     /gene="rapZ"
                     /locus_tag="PC700_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDF2579483.1"
                     /translation="MKFVIVTGLSGSGKSETMRALEDMGFYCVDNLPPALITKFAELC
                     YQPNSSIDKVALGIDIRGRKFFEALHESLNYLEKENYEYEMVYLDCNDDVLLKRYKMT
                     RRNHPLAKDMQIPEGIKMERKIMEPLKELSTCIIDTTNMKPKDLKEEIKKIYSSGEDN
                     PNLTISVVSFGFKHGILADADLVFDVRFLPNPYYVEELRAKTGDDKEVRDYVMNSKIS
                     EEFYVKLLDMIHFLVPQYIDEGKQHLVIGVGCTGGRHRSVTITNLIAEDLSNKGYRVV
                     KKHRDSMLR"
     gene            complement(71119..71832)
                     /locus_tag="PC700_000152"
     CDS             complement(71119..71832)
                     /locus_tag="PC700_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDF2579484.1"
                     /translation="MKILADYHTHTLYSHGKGTIEDNVKVAISKGIKTIGISDHSYKH
                     IAYGVKKKDILKMREEVDYLNDKYNDINILLGMECNILDDKGNIDMDDKFIRDFDYIM
                     AGYHFGSTPTSLRSLLNHCNNYIFKTEKAKEYNTKAVINAISNNDIFIITHPGDKGDV
                     YIEEVAKTAKKTNTRLEINSSHSFLNVNQLNKIKGLENKFIIGSDAHCPERVGDFKLA
                     MKTISDSNLDISLVENIII"
     gene            complement(71836..72750)
                     /gene="murB"
                     /locus_tag="PC700_000153"
     CDS             complement(71836..72750)
                     /gene="murB"
                     /locus_tag="PC700_000153"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDF2579485.1"
                     /translation="MNNQDIYINLLNILSKEDIKVDEPMKKHISFRVGGPADILVRPR
                     TEEQLKNVLKLVKEESIPYLIIGNGSNILIKDGGIRGVVIELADNFNSYEINDTRMTA
                     QSGALLSVLGKALQKQELKGFEFASGIPGTLGGALAMNAGAYGGEMKDIVKSVRLMDM
                     EGNIFELSNEQMEFGYRKSIISKNGYIALSAELELQEGNYDEIKSLMDDLATRRITKQ
                     PLNFASAGSTFKRPTGYFAGKLIEETGLRGLTLRGAQVSEKHCGFVVNQGEASAKDIL
                     DLIYVIKSAVYAKFGVMLEEEVKILGED"
     gene            complement(73023..73793)
                     /locus_tag="PC700_000154"
     CDS             complement(73023..73793)
                     /locus_tag="PC700_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDF2579486.1"
                     /translation="MSKGFLTPGETAQATISAGIKKANISTQNAILLGLFGGAFIGFG
                     ALGSMIVSQTFGKIDPGVASFLSAMVFPVGLMLVVVAGAELFTGNTLMAIALMDKKIT
                     LGKMLRNWGLVYFANLVGSILLVALVFYSSTLTGDAATKAIAVAEAKVNSTIASMFLK
                     AILCNILVVLAVWMATASQDIMSKLASCWTVIMLFVLCGFQHSVANMFFIPMGMALGA
                     NITMVQFITNLVFVTLGNIVGGSIVVGGIYYLCYVKNS"
BASE COUNT        20884 a        15677 c        11023 g        26329 t
ORIGIN      
        1 aaataaacat ctcttaaaat gaaattctgt aaacataaat gattttttag tttgaaggat
       61 tagattaaca aaattataaa tttgcaaata agctatatta aaaaatataa taatttttta
      121 tataacttat tcttttataa cttttaattt taaaagagga gaatgtatat aagagtattc
      181 aatgtactag aaaaaatcaa aagaaatatt agaagaaatg gatattgagt aatcaaataa
      241 aagattttga tattacaaat gatatgtttc aagaaagcat tcctatcaca acaaaaacta
      301 ttcttgaaga catgccttca aatgatgaat taaatcatgt tttttcaaaa ggttgtgaac
      361 gaaaaatgaa aaagagaaaa attgtattaa tcacactact attaataggt gttttgttat
      421 taggaagtat cctatataat ctttttttag taaaagcagc taatatttca atgctaaagg
      481 aatcttggaa ttttgatata ccaataccta acaaagaaat tgaagtattt gatactcaag
      541 atagtattaa tggagatgga caaagttatt ttattcaagg attttctgaa aagaatttca
      601 aaaaagtttt taatttaaaa ggtggtatag ttgtaagcaa agataatatt aatgaaatag
      661 aaaaatatat tgataaattt aaaagagatt ctgttaacat taataaaagt aataaaaata
      721 aaattgaaga agattttaaa aaatataagt tagaagttaa aaaagatgat aagtatatat
      781 ataagagaaa ttatgaaaac tatgtagttt taattataaa aaaagatgaa caaaaattat
      841 acagtttaat atggaatcag taggtacata tgtattatta agaattataa ataataaaaa
      901 taaattttag tcaatctaca aaaacctttt aaattgtctg gaaatcacct cctcatttgt
      961 tataaaaaca tacaacaaat gaagaggtga ttttatggtt tttaaaaaaa taattataag
     1021 tggatatgtg ctttcacatg tttatcaaca tttgtaatgc ctatatttgc taatgaatta
     1081 taatataaaa atattaaata atattcaact atagattatg taaattatcc tatgaaaaaa
     1141 ataagttgta taaaatctgg caactgtaga ttgacgattt cttttaaaat tacaattata
     1201 ataagtttta tagaattaaa aataaaactt tagagatttt tgataacatt gtagactaaa
     1261 tttgccaccg aaattacttt acagtggcaa atcggtggca aatacaatat attattagta
     1321 tataatctat ataaacatta ctattgcttg attatcttag ctacagtttc aacttgctca
     1381 tcattttcca aacttatatt aagatcttca tcaatgatag gtagcttaaa agtaatggat
     1441 tttagccatt gtccattagg ctgcttttct tcataaattt gaatttctga aatgagtgct
     1501 tctattaact gcctgcgttc aacatcgttc atgactttat aaagcttgtc aaaataaatc
     1561 aatactttat atatgttatc accagtaagt ttctcaacct ctatcgtttc tttttttgct
     1621 ttagcttcta ttaactgact ttctaattcc tcaattttat cgtacatacg ataaagccta
     1681 tcatctaaat cattttttct tctcttataa tgtttatcct ctacatctaa attatctatt
     1741 tcctcaataa gcttatactt tgtagaatga ctttttctta gttcattttg gtaattatct
     1801 atctcatttt caatagcaga tgtatcaacc ttcatgttaa ttttttcttg tatcatagat
     1861 gcaaatttag ggttgcttac taacttgatt attacctcag caacggcatc atctaacaat
     1921 tcttctctaa tttgtttatt ataggtacat ttatgtcctc tattcataag tctatgctta
     1981 caaccatagt aatagaagtc tttatacttc gtcccatctt ttttatattt tatagattta
     2041 ttcccaaaca ttccagctcc acatatcgga cactttacta ttcctgataa taagtgtgtc
     2101 ctcatgttct tgcccttatt tacatgttca tactttttag cttgtgcttt cagtttaact
     2161 tgtgcagcct gccacaattc atctgggatt attgcttcat gaatgccttc tgctactaaa
     2221 taatcgtcct gctctacttg tctgtattca ttccttgtac catgaacttt ttctaaagtt
     2281 cttcttccaa atgctatttt cccgttatac acaggattct ttaatatttt tcttataaga
     2341 cctgcgtcaa acaatggatt tttcccattt tgtcttggta ttttccttat accatgattt
     2401 tctaaatatt tagaaagacc attagctcca atacttgtat ttacatattg atcaaaaata
     2461 gttcttattg caattgcttc ttcctcattt attatcaatt tcccatcaat aagttgataa
     2521 ccatacggag caaatccacc attccattta ccctcttttg ccttttgaat gcgaccttcc
     2581 attgtttgga cacgaatgtt ttctctttct atttcagcca ctgccgataa aacagaaatc
     2641 atcaatttac ccgcatcttt agatgaatca attccatctt caacgcaaat aagatttact
     2701 ccaaaatcct gcattatttg taaagtcgaa agtacatcag cagcatttct tgcaaatctc
     2761 gatagtttaa atacaagaac gaaagacact ccatcctttc cggattttat atcttccatc
     2821 atacgagtaa actgaactct accttctata gattttccag acttacctgc atcttcgtat
     2881 tcaccaacaa tctcataatc attatagaga gcaaaagcct tcattcttga tttttgtgcc
     2941 tctaaagaat aaccatctat ctgtatggat gtagatactc ttgtatagag atatactttt
     3001 attttttctt ttgacatagc attaacctca atatccttat tatatattta tatattttta
     3061 ccaataaagg tttggacttt aatctcaagt atattatagc acttttattt ctctttctca
     3121 atccgtgctt ttagctatat caaatttatt cttatcctct gtatttttta ctggagtagg
     3181 atttggaaga ttctctaaat ctaaaactcc agcatatttt gtaatcagat ttgctatcaa
     3241 atcagccaat ccattccagt cattatccac taatacacct cctccacttt aaattcttta
     3301 taaccaaata ttatttttct tactaagttt tatgacattg gtaggtgcat tggctgctac
     3361 ataggaatct cacctccgcc ccttgtttca gacgaggcag cttaaactat tgaagtatca
     3421 ttatcaccac ttacatcatc gaaataagct gccacgctta tcttttatgt attcctatct
     3481 atcgctcatt ttcttctaac ctcggcatta tgccggtatt catttgaacc gattttcatc
     3541 agcagaaaag tcttggcgta ggtcataact ctcagtaagt agataaagtc ctaccttggt
     3601 ctattcagtt gtcaaagatc aatatttgaa agggactgtt ttctttacat tttcctattt
     3661 gccataagca ggcgtatctc ctactcaaga aagtaaagga ggtcaaactc cccttcactt
     3721 tacataagga aaatttgccc cctttggtta ccaaagtttt aaagttcttc gataaatttt
     3781 ttcagctttt caagaatttt gtttcttctt ttgattaaag ccggatgtgt aatacttttg
     3841 agctttgcta ctgaacgaac agtttcatca ttgaaataca agcgttcaat aatatctctt
     3901 tcttctgcat tgagccttgt tatagcactt cttactgctt caatcatcat ctgtgtttca
     3961 acaatctttt ctacatcaac actttcatca acaatattat ctgtaaagtg tccgtcatga
     4021 tccaatgctg aaaaaaagag caagtggttt ttcctgtcca cctgctctaa atacttctcg
     4081 tgttcttttt ctcgccagta gactatatat atatcttcac tgacttttac cttttgtcct
     4141 ctgatataaa gataatactc ttttgccata aagtttcctc catctctttt ttatctgctt
     4201 gtttttcaga taaaaaagga ggacttctgc atcttggcat aagaagtcct ctgaaatgaa
     4261 agaataaaaa tatttcttta tatgaacttg attgtttcaa ttcgcacctg aaacaatcat
     4321 ctactataca tattataaaa aagaggaatg tattgaattc taaatatttc taacattaaa
     4381 cgtttagatg cttccatatt tatttcctcc attttatcaa taatctaaat aaccatcata
     4441 ttcatctact acttatataa ttgagtttta ttaaactttt taagtttcta ttctatcaaa
     4501 tatttaaaca gtctctgcat ccgaaaagac taactcatat agttttacta acaatatttt
     4561 cattagcgtg ttcaaatcct gatttcttgt atcaattatt ttctgttcac tttcaataag
     4621 aaaaaagcct ttacttccat ggctaataat aagtctctat ttttcaataa ctattgcaaa
     4681 attatatctt gtttaagaaa atattttaat tttaaagata aaaaattctt catcctgcaa
     4741 tgctttatat tctatattgt aattattcag aatattactt acaatatata aacctaatcc
     4801 attactattt tccttattta aatcaaattt aacatcaaat attttatcca tattcgaaat
     4861 tttattgtta ccgtatgaat tctctatata taaccaatca ttaactatcc caatattaat
     4921 taccccattt acatcagtat attttaccgc attactaatc aaattagata gaataatctt
     4981 taaagctgtt tttcctatat aaacattctc atctaatata taattattga cagttatttt
     5041 cttttgattt gctaatattt catacttttt taatatgtct tctaatgtat catttatttt
     5101 caaatattct tcatcattat tcaaattttc tatagaatga actgataata tttgagaaat
     5161 attttttgtt aaactatcaa cgatttcaat acattcatta atataaatat ctctttcttt
     5221 atattttcca atattgtatt tcatgttttc cagtattatt ttaagactag caagaggagt
     5281 tttaagttca tgggatgcac ctttaaaaaa gtcatatttt aacttttcta attttaaaat
     5341 ttctttattt ttaaattcca aatcgtcaat tgttcttaat aaagtagaat acaaatcatt
     5401 aatttgttgt tttaattctc caacttcatc attagagcta acttttaaac gcgttttttt
     5461 atcaagtttc atcattttat cagtaacaat ttttatttct tgtatattat tttttattga
     5521 ttttgcataa attaaagaaa taatagcaga aaataaaatt gatattaata aagaatatgg
     5581 taaaaattta aggcttaaat ctttcgcatc tttttgcata tcagctgtag aaacaaattg
     5641 tagatttgtt tttttaccat cattaagctt tatttctctt tcttcaatta tcaaagaatt
     5701 actatcactt tctaaattaa catttatatt atctttgatt tgtatttcat tcttattatt
     5761 tttttctttt ataaatgctt ttatttcact actcttagaa taaagctcca aagtttgttc
     5821 tatatatttt atttcttttc cattcatatt actagaaatt tcatttgcca tattatgaat
     5881 cttttctttt ctcgtctcca aatatgtttt aggaaagata aaaaaaacta atgaatgaac
     5941 taatataatt attattccaa gaacagaaaa tatttgtatg aacatttttg gaaatatctt
     6001 taattttttc attttctctc caatttatat cctacatttc ttatagtagt tatacagtct
     6061 aattgtaatt tttttctaag ttcttttata tatacatcta ttactcgatc ataaggagtt
     6121 tcttcgctat ctttccatac ataatcaata atttgcattc ttgttaacac ttgtccatta
     6181 ttatccaata aacattttaa tacttctagt tcttttgcat ttacatctat ttcttcacca
     6241 tttattttag ctgtatagct attaaaatta accgatagat cattatattc aaacttctct
     6301 aaatgtccat aattcttctt aattaaagaa tctattctag cctttaagac cggcaatgaa
     6361 aatggctttt ctacatatcc gtctactaaa ttagtaaatg catcaatttt atattcttca
     6421 tcactaaatg cagtcaagat tagaattggt aaattgcttt tctttctaat ttctttcaac
     6481 acttctaaac catttataaa aggtatctga atgtctaaga taaccaaatt tatatcgcta
     6541 ttaaattttg acaaggcttc tcttccatct ttagcttgaa taacagtata tccaaattct
     6601 gaaagatatt cacttattcc ctctcttatc atattatcat cttcaacagt taatattttc
     6661 atatatacct ccacttaagc ctctacactt atattataca gcatattatt tgatatttaa
     6721 aagccaaaaa atttttggtg ctaaaaagca ccgaaaattt taattattgt caattaataa
     6781 ttcttttgga ttccttctta atatattgaa tgaagaaata atcaatgata caagaagtac
     6841 taaactcata aatataacta cataaattat gaatttaggt aatacattta tatctaaact
     6901 tgataatgtc ttgttaaatc cttcagcttc tgccccacca cctaattggc tagatgcaga
     6961 ttgtctagct atttgtttag ctatatcacc agtaacctta ttcaatatat tattcccaag
     7021 cttatcagct gtatattgag ctaagaaata agaaccaacg aatgcgggga ttgatataaa
     7081 taccatctca attaggaatt gaccaaaaat ttctaatttt gatataccta atgatagtaa
     7141 tactgctatt tctttctttc tagcattcat ccataagaat aataataatg aaactacaac
     7201 tcctgcaaat attaatgagc caacaaataa tttatttgaa attgagtaga tacctgatat
     7261 agattgttgt aatgcagggt aatttgatga acttttaatc aaattatatt ctctccaatt
     7321 tatatctaat ttcccaagat cttttattac actatcaaga ttcttatcac cttttacaaa
     7381 gaatgttgca tcttggtata cagctgtatc ttctgtatta ccataaactt tagcagctgt
     7441 atgaacatca gtgattaatg tattttcgta tagttcttgt gctgcactta ctccaccgct
     7501 attatgccca tcgaatagac ctttaatttc tacttctact gtttcatctg cacctttctc
     7561 attatcagca tcaaataaat tagattttat ttttatctta tctccaactt taagattatt
     7621 tttttcagct aaatctttat gcattaagat tttattttta tcttcatttt ctaaatgctt
     7681 tccttctact aatttatatg cttctgatac aaattttgtt tcttttgatg agtcgttaac
     7741 cccagttaac ataactgttc ttttaaaatt cttcgctctt tcaggtgatt gattcgcaag
     7801 agtctcttga gtttcaataa tatcataatc aactaaatct gcaacactgt ttattctttt
     7861 tacataggag tctatactat ctgtttgaaa tatcttttta atatcttcac cttttacatt
     7921 tcccccacct ctaggtgttc ctggatttac ttgtctattt atctccatag aaaaactatt
     7981 tgttatatta gcaaatgttt cttttgaagc tctgtccgta gcatctttaa tagataaact
     8041 aatgagactt aaagttgaca tagataaaat aactaatata ataattaatg attttaagct
     8101 ttttctagtt acataagcaa atgcattttt tatcattatt caacctccaa attcatttta
     8161 tttacttttt tcaacttttt accacttagt tctaaaatga tatccgcaga atctgctact
     8221 tccttactat gtgttacaac tattacacat ttatttctat ctttagctaa tgtctttaat
     8281 atattaatta tttctccagc agtaacactg tctagattac cagtaggctc atcagctagt
     8341 attattggag catctgatac caatgctcta gcaatagcta ctctttgttg ctgtccacca
     8401 gataatttca taacatttct ttttatttgt tttttatcta aacctagttc aaacaagata
     8461 ctttcatctg ctgatttatt tactaatcta atattttcaa ttggtgataa ataatctatt
     8521 aaattatagt tttgaaatac taaagatata ttattttttc tatgattact atatcctttc
     8581 ttttgtatat cttcattctt aaacaatatt tttcctgttt gaggtttatc aagtccagca
     8641 agtaaggaaa gaagtgtaga ttttcctgtt cctgactttc ctactatcgc ataaaacttc
     8701 ccaagttcaa atttttgatt tactcctgac aaaacttttt ctttagaatt tgcataacta
     8761 tatgctacat tctttatttc taatatatcc attattttct cctaacttat ttttgataat
     8821 atttctttag gcttcttgat taatattaat gaagaagcaa atacaactga taaaacaata
     8881 atacttatta atattaaata actttgtcca agtgttgtta tatttaacat aaaactacta
     8941 ttatttatta aacttccacc ggaaatcatt gagtcctctg agttaatgaa tccatctaca
     9001 attactttta gtaatacatt tcctaaaaat aaggaagata ttatacttgg tatcgatatg
     9061 aatattaact cgaatataaa ttgcattata atttgtatct tagatgttcc aatagataaa
     9121 aatataccta tttcataaat tctttctctt aaccatagaa tcaagattaa tgaaagaaca
     9181 accatcccac ctaacataat agaataagtc attattttta ttatatgttt tattccactc
     9241 actgactcta aagactcttc aaacgcatta gaatcttttt caacaaaata ctttgactca
     9301 tcaattttaa gctctttcaa tttgtttaag gctaagtctg tagattctgc actaccagaa
     9361 tacattaaaa ttttatttgc gattttatta ttttctgatt tatttaatat ctcttggctt
     9421 gttgaataat ctacaaacac catattttca ctaaaatcag aagataatcc tgtatatgtt
     9481 tcctgttttt taccagaaaa gatccctata attttaaatt tatgactttt tatttttcca
     9541 cttttttcaa tatctagtaa ttcaagatca acttcatcac ctaattttag attgttttgt
     9601 ttagcaaatt cttcatgaac aataattgaa ttcttatcat tttctcctat atttttgcct
     9661 tctttaattg taaatactcc actactaaat aaaatatttc ttttagtatt atttgtagct
     9721 tcgagtgaaa caacattctt aaattcgtca gataaatctt ctctatttat tctttgttct
     9781 ccgctaacta ctttagcatc ttttagtttt gctaatccat catattgaat tattttttct
     9841 tcaatttctt ttaatttttc aatatcttta aattgattaa cattaaaata tttaccatct
     9901 ttttttgtta ttgatattga agaattagaa ctttcatata aagccttttc tatttcattg
     9961 cttgatttca ttattgttaa acaagaatac aagcaagaaa gaactatagt taatatgata
    10021 aaaattatta aagttctatt tctctttctt gtaatatatg ctatagcatt ttttatcact
    10081 ttcctaacta ctcctctcta ttttcttact ccataactaa ttgcatccac tggacaagca
    10141 tttttacatc gtccacatct gatacactcc agatgattgc aattctcaac tggatctata
    10201 ttcatttggc aaactttttt acattttcca caattaatac acttatcctt atccaatcta
    10261 tatctaaata ttgaaatagg gttaaaaatt gagtaaatag ctccaagtgg acatatatat
    10321 ttacaaaaag gtctatatat aattatagaa agtaacaaag taactattag aatgattcct
    10381 ttccaataat acaagaaacc tatcgcattt ctcatacttt tatttaataa aacaagtggt
    10441 aatcctcctt ccagcatacc aatcggacaa ataagtttac aaaaatacgg agaaccctga
    10501 cctaaaatat caaccaaaaa taaaggtaat attattacaa atacaagtaa tattatgtat
    10561 ttcaattttc tcaaatgttt atcaaactta aatgtttcaa cttttttaaa aaagggaatc
    10621 ttatgtagca aatcttgaat caatccaaat ggacacagcc atccacaaac aaatcttccc
    10681 accaatgctc ctataaaaaa caaaatccca actatataat aactaatctt atattttgaa
    10741 tttccaatta ctgcctgtag tgatcctatt ggacaagaac cttttgctcc cgggcatgaa
    10801 taacaattca tacctggaac acaaattttc tttaaattcc caccgtagat tttaccagtc
    10861 ttaaaccctt caaagtaaga attagtaatt aaaaaccaaa cggattgaaa taaatgccta
    10921 aaacgatctc ttattttata tttatctttt tttgtactac catttataat atttttaatt
    10981 ctatccaatt ccaatacact ccatacaaat tcttatcgcc ttatccaaga caactttagt
    11041 ttctccatta ctatgaccat agtaaatcat cgccatacta ataaagataa ttaataaaga
    11101 caaggcacgg ctttttaaaa acttattaac catataccaa tcaccgcttt acttttattt
    11161 attttttatt ttttccaatt ctttttcaat tacctgcttc cattcctttg aactctttgc
    11221 cccagaataa gtatcaccta caatatttcc attactatca acaaagaaag tctctggcat
    11281 agcctgtata ccattcaatc taccattaaa gtttgtctta tctggcatta agaaaggata
    11341 tgaagccttt gtcttttcat gtattaactt tgacttttca attgcttcct tgttttcacc
    11401 gttatcatca acagtatctg taacaactcc aacgatattt acacccttgc ttttcatttc
    11461 attctgaacc tcaaccaaat caggaatttc ctttacacaa gctgtacacc aagttgcaaa
    11521 tacatttacc atagtaagat catacttctc aaaatcttta cttgtaaaat ccttgccatt
    11581 tatgtccttt gttgatagtt tacgtaaatc tttaaccgat tctttgttaa atgcttctgt
    11641 attttctaaa tcagtttttt ctgatagcac aaaaccattt ttaggacgtt cttttttatc
    11701 tataatttga atctcagttc tttttaattc atccagaaga ttactttcag ctccgctatt
    11761 tgtgcttaaa tagcaatcgt attttccatc acttgaaact cctattttag tatgagtatc
    11821 acattttgta attttagata ttttttcttc tgatgtattt ttttcgaata taccaatagt
    11881 cccaattctt tcaagctcat tttgccaatt tttatatcca tcgcccatct tttctataac
    11941 tgcattcttt tgttcttctg tcattttttc aaatgtcaaa atagcatact ttagttcttt
    12001 atctatagga ctttgatcat ctaacatcgc tatcttttta tcatccatat actttttaaa
    12061 tttgtctgaa agtttaaatt taagacctaa atattcataa acatattctt tttttgtttt
    12121 taaagtataa tctgatggct taaatgtttc atttttatcc ttctcattag ttgacatacc
    12181 tgcctcttca gaacttatag attgtccttc tttaatttta ttgctttcat tacttttttt
    12241 acacccaact gtacttaagc tcaaacataa cgtaagtaat aggcatgctc ctcttgatac
    12301 tatttttttc ataaattttt cctcctttat tcctccttta tttttacaca atagcagttt
    12361 ataactactt tcaataatat tctacacacc ttttatgaaa cgttgatgaa atataatttt
    12421 ttatttttcc aaactatggt ataataatat aaatattatg cttaaagttg ttcttacacg
    12481 attagtatat gatcgattag tatatgagca agcacagtaa gtgtacggac acttacgaaa
    12541 aaattgatga agcagccctc tgggatatgc ttcttttttt atcaattttt aacttgttag
    12601 ggtataccct aagaccccga aatacattca aaggaggatt tacctatggc aaatagaata
    12661 cgaaatgaaa gactggaaat taaactaact gaagaagaaa aggctctttt tgaggagaaa
    12721 cgaaagctcg caaggtgcag aaatatgagc cactttatcc gcaaatgcgt tttggaaaag
    12781 gaaatatatc aagtggattt agaaccgttt agagatttac agggcttact gtccaatgct
    12841 acaagcaaca tcaatcagat tgcaaagcgt gtaaattcca ccggcataat ctataaggac
    12901 gatataaacg atatgaaaaa gcagattgaa tatttctcaa aagagttgtg gcaaatacat
    12961 tctctgcttc ttaacagaac ttctggaggt gattaaatct tatggctata acaaaaatac
    13021 atcctataaa atcaaccctt aatcttgcca tttcatatat tgttaatgga aaaaaaacag
    13081 atgagcaaat cttagtaagc actcataaat gccatcaaga aactgctcat acccagtttt
    13141 taagaacacg aaatgatgtt ggaacaaacg gaactgttct tgccagacac cttattcaat
    13201 cctttttacc cggagaagca agtcctaaaa tggcacatca aatcggtctt gaactgtgta
    13261 aaaagatatt aaaggacgag tacgaatttg tcttatctac ccacatagat aaagggcata
    13321 tccacaacca tatcatcttc aataatgtga atatggtaac aggcaagtgc tatcaatcca
    13381 acaagaaaag ctaccacaaa atcaggtatc aaagcgataa gctatgcaaa gaaaacaacc
    13441 tttctgtgat tgatgagttt tacgaaagct ataagagaaa gtacaaaaca aatggcaagt
    13501 catggtatga aaatgagcaa tctaagagag gtacttcttg gaaaagcaga cttcaatttg
    13561 acattgatcg tcttataaaa cagtctaagt attgggaaga atttctaaaa aaaatggcgg
    13621 agcttggtta tgaaataaag tatgggaaac acatagcctt taaaccaaaa gataagacaa
    13681 gatttacaag agctaaaacg attggtgagg actatacaga agaaagatta aaagagcgta
    13741 ttacagaaaa tcaatctatt acaactccac ctgtcaaaag acgaatcgga aatatcatca
    13801 atatgaatgc caatgccaaa gtgaaagaga gcaaaggcta tgaatattgg gcaaccaaac
    13861 ataacttaaa tacaatggct gagtcagtta tttttatcag agaacatggc attaactcca
    13921 ttaagcagct tgataaatac attcaaaaaa gtgctgaaga aagacaagac ttacaagata
    13981 aaatgaaaga aattgacaag gatatgcagc tactttccga tacaatggag caggttcata
    14041 ctgttaaaaa atatcgagcc tactacaaag aatataaagc aaacccttcc gataaagcat
    14101 tttttgagga gcataaggct gaaattacac actatgaaat agctctttca aagctaaaaa
    14161 aatcctattc aaagctgccg aattcaaagg atattttaga taaacttaat aaactgcaag
    14221 aaaaaaagaa tacccttatt caagagtatt cttctacaaa atctactatg gacgaacttt
    14281 atcaaatacg aaagaactat ggaatttaca tgggtaagga gatggaacga taactctaat
    14341 attatatagc tttcttattt ttataatatt aaattgaaat gcctatcaat gatgcaagca
    14401 tttttatacc taaagctaaa tcaggaatta aagctacgga agtagtgata aaatgcatac
    14461 tttttaaaca agtagtcaat acttctttct tcggatgctt ctgcttaatt tcatttttta
    14521 tattatctaa attctgatta agtattgatt tggagttatc atcctccata cctttaatta
    14581 agtcttctac attgttaatt gccttgatta cttcttcagt attaaattta ttttcttggt
    14641 cagcgttaat cgtgcttccg tgttccgcaa tattgacttg aactccagaa ctattaacat
    14701 tgatattata tagagcatta ttttcatcgt accccatatc ggtagaaata tctttcatat
    14761 aattttcaac ctcaagtaca aacggataga ccattctatc gccaaacgcc tttgccatat
    14821 cttgatattt attactgcca gcataatacc agccaaaagt atatgtaggt agattatttc
    14881 ctgttataaa ggaaaatgct ctatacagta gatacgtcct tttattacta tcattgccaa
    14941 gattcaaaac tttacgtcca taagaattac ttatcgtatt taataagtca tctaagccat
    15001 caacatcata ttctaaacta tgcaaatact cttttatcaa caatgtttgc tctatataat
    15061 ttatcatttc ttccaaattt gcattctgtt ctccataata tgaattcata acatttgaag
    15121 ctaatatcct aaattttcta ctgatttttc taagttcttt tttactaatc tccatctatc
    15181 atctctccac aaaacgggca ataactacct tctaattttt ttatactact tattctagcg
    15241 gttttgccac aacacaaata tttcttttct tcataatctc cagtttttct tcctatcgga
    15301 agttctgctt cctttttaat ctcttttcca cgtttaaact ttatactttt actattccta
    15361 aaactttttt caagattctt tgaaaaatca tttagcattt cagcaacttg attttcaact
    15421 attcgttctg ctaattcata aatatcatcg tctaaataat tctcgtgtgt taaaccacaa
    15481 ttaggacacc aaatatctat tgtgctttca gcctcaatat cttccaccgt taacataaat
    15541 ttttcactac aacttggaca ttttaattga acatatccct cttcatccgc tggaacagaa
    15601 atttcaaatt ttatatcatt agccatttaa atttcatctc ctcacaataa aagttataat
    15661 ttcaccactg gtagactaca aggtcgtgca tctttacgta caataacaac actattctca
    15721 gcacgtgtaa cagcaacata taatctagcc ttactttgtt cttttagaga tgtgttattg
    15781 ttaataatcc aatcccacat cggttgagtc ggatatataa gtactcgttg gaattccaag
    15841 ccttttgatt ctccaaaatt ataatatgga tattcattat tgacggatgt ccttttattt
    15901 tctctcaact gcattgcatt aaatttctgc aaatacttct ctacatctgc tttatcaatt
    15961 atcataacac catctgattc atcttcagct ctacgcacaa aatttgtaga tgttctctct
    16021 ggatacagac tattcgcaaa tttacatata atatcattgt ttctataagt acaattaagt
    16081 gtcgtttcat caattcttac atctaatttt ctgcactcat tttttaaaaa ctcatctatt
    16141 ttcccacaac tatatttttt atattttttt gatgagtgag ttgaatatgt gcattgtcta
    16201 ggatccccga ccattatcac ttcaattctt gaagctatta aacactttaa tatttccaaa
    16261 tcatacccca ccatatcttg gacttcatca acatatatac agtcatacat tccactgatt
    16321 ctttcaaaaa cttttccatt acatttatca tttaactcta caacacactc tgataattta
    16381 tcagtatata ccctgttatc tttggcaaaa tatttgttgg atgttttctt tacgtacgta
    16441 ttgttctgtc catttacaaa tataattcct tcaattcgtt ccttaaatct acttccttga
    16501 taaggtctaa taccatcttt taacaaaaaa gaaaaccatg tagaaactgt aatattatta
    16561 ggaattacac caactttatt cttaattcgg ctaattattt gttgctcatt tgtaatagta
    16621 tatgttagga ttagtatttt tttatgagta ttaagagaat cctctataat ttttcttgtt
    16681 tttccagatc cagcagcagc tatatataat ttcccactca tattagaata ccctccttta
    16741 tatattgtgg aaattctata gctttatcac tttcaaaaat ctttaaagca acttctgttt
    16801 tatgcccctt catatattta agtaaatcat ctggatcttc atatgattta ttcaatatct
    16861 cgtttaaaat agacatggaa ttcgatctat atatacacgg ttccaaagta ttatagttgt
    16921 aattttccaa agaaccatta tattcatggg tactatcatc aaaatatata tttatattct
    16981 cacaacaatt atctcctaaa taatcgctat attttctatt tagtgcatca atatttccat
    17041 cattatcggt tatcactgac acacgtaatt ctagcaattt tgcgatttcc aaaaatctta
    17101 aaaaagaaat tccaaccgat attatatcta ttccatcatt aataggcaat ttaccattat
    17161 gagagtccat atatgctctt tgaacaatta gttcgtccga atcaccttcg actaaaattg
    17221 ctttatttga taataataat cgtaacgtat cataacctgc aattctttta aaaaataaat
    17281 ttgtttcatt tgataaatca cctagccgta gtgtttttcc tcgatttagt aaaattaaat
    17341 tttcaagtcc taatttattt gatacaaaac tactatgcgt agtaactatt atctgttttt
    17401 cttttactga ctcatttact ttatctagga gaatatttaa gtttgaatat gacaaatggc
    17461 attcaggttc ttctaaaagc acaatctgct tattttctat tttatgatca cttaatgcca
    17521 attgtgtttt aacaatacat tgtgtacctt taccaatgtt attatatggt atttcattta
    17581 attgggttac caagctgctt tcccatgaag ttttcgtacc gaaatctaca gaaattgtca
    17641 attcgttatt tgatgtttcc gttaattttc tatttatatt agcaatattc tcatctctag
    17701 aaaattgttc tttcataagc ctatgcgctt gcgatatatt aataatttca ttatcatcta
    17761 acagattctt tactattctc gatatgtata tatctgaccc actggtagga gtatagcttg
    17821 tcgtatcaat cattgcagac ttaattggaa tgctccttgc agttatattt tctctggcaa
    17881 aagacatcca acttacctca taatattcaa ttggcaacga tgttatcttg ttcttacaaa
    17941 gcattttata ctcattgcta tatttttcat caaaactaat tgataaacaa atcccagggg
    18001 tgtctttagt tttttcagaa tttccatcac ctaaaaaact tggcatttct gcatcaaaat
    18061 acgcttcaat aattatttta ggaggttcta cattcactcc ttcgttgact tctctaatat
    18121 agttttccac tatattctta ttgaaaaggt actgagtaag ttcattccta atatttttcc
    18181 catgataagt tccagttaaa accagattaa tagcttccaa aattgtagat ttacctgttc
    18241 cattattgcc aactaatata ttaatcccat tttcaaaatc aactataaac caatctgaaa
    18301 agcatttaaa attctttatt tttaatttcc taattgacat tttatccccc ttctttatct
    18361 aatataaaaa tcatgatttt agaatataaa tattgataac tatataaata cagttatgag
    18421 tttcctgttt aatcacaatc ataaattttt tatgctacat cccttaatat tttttcaagc
    18481 aataatttaa caataagttt tatgatcctc tagctcctga aaataaatct atgactgtaa
    18541 acttagactc ccattctcct tacatcttca ataattacgc actccataat atcttcaata
    18601 tcacaatcca aagcattaca aattctaagc aaaacatccg tagttacatt ctgtccattc
    18661 ttcattttgt aaaatgtact tttactcact tttgcctttt ccatcaattc attatttgtc
    18721 atatctaaat caatgagttt tttgaacaat cttttataac taaaaaccct cattcttatc
    18781 ctccgttcaa gaaaactata tctagtatat tataccacaa aatatacttt tttcataaat
    18841 atattctctt taatcgaact tgcataaata aaaaagagcc ggtgcagtcc taagaccaca
    18901 ccgaccgtaa atttatctct cagcttcttt gcttgcttct ttcttttctt tcggtttttc
    18961 cttatcttca gcctgatatt ttttgatagc tccaagtata gattctttct tttcttcctg
    19021 tcctttcatc tgttcctttg cctttaacag ttttgaagaa agtatcctga tattgctatg
    19081 ctccttgccg ttatcatcaa tggaagttct gatctgccca aagagtttga caaaatctcc
    19141 ctgcttaaag tcctttggaa tatcactctt ttctccataa acggagcagt tgtgatataa
    19201 cttatttccc tcatcatctt tagatactac ggagaagttt gctaccttaa aggtttctcc
    19261 gttcttattt tccctttcaa ttacatcaac ttctccaacg atatttccga caatattgat
    19321 aagattatcc ttttcttccc gaacataaag caggtctttt atctgcccct gtccccttaa
    19381 atcctcaatc atatagtcaa actcctcatg cagcaaatta aggctatcat tgtccatata
    19441 agcgtcatat agcttatcta aagcactttc atcattgata cccttttcca tgcttataat
    19501 cgctttaaca aagtctttat gattatcact taacatatct tcaactctat ctctcattgt
    19561 tttgtaatcc atgtttttat ctcctttcat ggtaaaaggg aggttgccct ccctttatct
    19621 taaaaattcc tgctctttta ttttttgttt ttcttctgtt ttactttctt cctgataggc
    19681 tcttatctgt cctaagatag acggcttatc atctgatttt tgtgtggttt cttctttgct
    19741 atgaagattg tcctccacat cataagttga ctcaaaataa tcactgtcct taaaatcact
    19801 atcatatcta tccggaatac cgtcattatc aaggtctttt gcaaacggat catagaagtt
    19861 accctcatcg tcaatatcaa gtcccgttgc tactcttaaa tcttcagaat caacataaac
    19921 caaatcacta aatgtagata actcaatttc atttttcata tttcttagag cttcattttc
    19981 tcccttattt tcataatcaa agctctctgt tttaataggc gtatcatcaa tgtactgtgt
    20041 ccaagttttc tcctctaaat taagctcata ttgaatacca tgtctttcat ccggtgtatt
    20101 ggtataggcg ataccgatat gctttaagtc aggatataga gtatgaaact catcataact
    20161 gtgattttct tcatactctc tgttacagaa gtcaatgatt gctcttttta catcttccac
    20221 aagaggatta tcatttttct tttcttccac ctctcccata tcgagcagct tatttaactc
    20281 tgcaagtctt aataccttag ttttcaactc atctgccttt tcaaaaggct tttttaattc
    20341 ctctttggca ttttcaagct gttcttttgt acttatgagc ttttcttcaa gcatatttag
    20401 tttctccggc attttctcaa gggcattatc cagtcttgtg atattaccgt ccgcacttgt
    20461 tccaagctct cctaaatgct ttgcaacacc gtttaagctg aaattatgag aattagtaaa
    20521 gaagttatag cttacctcta aatccatatt tctatacttt cctataacct tgctttcatt
    20581 gatttttacc tttgaaatag cttcaagtaa tctttctccg gctaatttct tatcggtaat
    20641 tttttctccc gcaatggtaa tagaagtaaa cttttcttca ccttccgctt taggctctac
    20701 attggatata tcttttttga cagcttctat gagcttttct gttcttgcga tttcttcagg
    20761 atagtttttt gccaccttat cctctaatct gtaacggtta gacttatagt ttgcttcaag
    20821 cattttaagt tttgtaacct cattatccaa atccatcttt tctttaatct ttggatcacc
    20881 cgttgccaac gctttaattt cggcgtaatt taagctgctt tcgtccacat cttctgccac
    20941 acgaacagga gtcttactgg tcataatctg tgaaatgaac ttttgcttat tctctatcgt
    21001 ctgccaaagg taggcatcaa aggtattctc tgtaacataa cgatagatat ttaccttctc
    21061 attttcattt ccctgcctta caattctacc cgcacgctgt tcaagatcag ctggtctcca
    21121 aggcacatcc aaatcatgaa gtgcaatcag tttattttgc acattcgttc cggctcccat
    21181 cttctgcgtt gaacccatta agatacgaac ttcgcctttt cttacctttg caaagagttc
    21241 atccttttgc ttatcagaat tcgcttcatg gataaaggcg atttcttctt tcggtattcc
    21301 cattgctaca agtttttctc taatatcatc atagatatta aattctccat ctccttttgg
    21361 agtagacata tcggagaaaa gtagctgtgt tgacctattt tcttttgtct tatcccaaat
    21421 agcaaataca ttttttacgc atacatttac tttactatca gggttatcag gaagtagggg
    21481 attgattaaa cgctgatcta aggcaagttt cttaccgtca ttggtaatct taagcatatt
    21541 atcaacatcc ggctctacaa ccctatttct gacatcatcc gctctttcag aaaggctctt
    21601 taggatttcc ttttgttctt cagaaggctc agttttaata acttcataat tagcttcagg
    21661 tgtaggaagg ttaagcatat ccgcagtctg aatatctgct acttccttga acatactcat
    21721 tagttcaggc aagttataga actttgaaaa tctcgttttt accctatatc ctgttccttc
    21781 aggagatagt tcaaaggagc tttgtgtttc tccaaaagtg gaagcccaag aatcaaaatg
    21841 ctctaagtga tttttcttta aactctcata ctgaagatac ctttgcattg tataaagctc
    21901 tgtcattgag ttacttacag gtgttcctgt ggcaaaaact atgccttttc ctcctgtcat
    21961 ttcatccata tatctgcatt tcataaacat atcggaggac ttaaaggctt ctgactgccc
    22021 gatacctgca acatttctca ttttggtata gagataaagg ttcttgtaat tatgtgcctc
    22081 gtcaacaaag agcatatcaa ctcccagttc ctcaaaggta attacatcgt ctttcttaaa
    22141 atcatcattt aatttctcaa gccttgtttc cagttttttc tttgtctttt caagctgttt
    22201 tactgtaaag ttctgatttc tgtcatgctt gtattcttct acatagttta cgatttcatt
    22261 aatctgatct tggatgtgct tttcctgata ttccttactc atcgggattt tttcaaactg
    22321 cgtatgcccg ataacaaccg catcatattc ccctgtggca atctttccga taaatctctt
    22381 tctgttcttc ggttcaaagt cttttttatc agccaccata atattagccg acggatatag
    22441 ctgcataaat tcacgaccga tttgtcctgt taagtgattt ggaacaacaa acaaagactt
    22501 actgcacatt ccaagtcttt tactttccat tgcggaagct accatctcaa aggtctttcc
    22561 actgcctacc acatgagcaa gcaatgtgtt tcctccataa agacttcttg ctatggcatt
    22621 tctttgatga ggtcttaaat caatttctgt attcattccc tcaaaagaaa gattgcttcc
    22681 atcatattct ctgtttcgga ttgagttaaa acgctcatta tacagtttta caagacggtt
    22741 tcttctttcc tgatcgttaa atatccagtt cttaaattct tcttttaata gctcctgctt
    22801 ttgtcctgca agcatggttt cttttttatt taagacagaa gttttagaac cgtccggatt
    22861 tataatctgg tcaaatacct ttgtttcttt aagatttagg gcatcttcaa tcagcttata
    22921 ggcatttacc cttgatgtac caaaggtcat ttctgcaagg tcatttcctt tatccctgct
    22981 ttttccctct acattccatt cacttgtaat atttgaaaat ttaaccttaa tatcccatct
    23041 tgcatatccc ggagttttta aagtttcaaa gatgaatttt tcaatatctt taatcggtat
    23101 ccaagtcgca ccaagacgca cattgatttc acttgcttct aattcctttg gaagaacttt
    23161 ggtaagctct gctttttgat attccaaacg attcatttca tagcttatca gctctttttc
    23221 ttttccattc tcaacaaagc caagatgagg aagctctcgt tccgtttgtc ttaactttgc
    23281 aaggtagctg tctacaatgg caatcttatc tctaatattt ccgcttaggt actcatcctt
    23341 tgttacatag ccgtacttat atgaattact gccgtttgca caagcgaaag gcaaatctcc
    23401 atcttcaagg ttaaaggata atggtctata aaagttttgt tcttctctga tgtttagata
    23461 aatttctcct ctaagttctt ctatcaaagt tggtctgtct ttacctgtaa ggctttccat
    23521 atactcaaaa tccacatagc ctttttcaga taccgataag acaagagctt caagggaagt
    23581 gtccacatgg tctatgactt tggcttttgt aatggttctc tttgagaaaa tatccccctt
    23641 tgccttgacg ttttcttctt catcaagaat ttcaatggaa gatacaagag ggaaattgct
    23701 atcctctttt aaggctctgg tattgcttaa attatttata aagccatgct tctttgaaaa
    23761 gttgtcatag acttcattaa gcttttcttg agctttcttt acctcatcat cggaatagtc
    23821 ctctttctgc ttgtagataa catctttaag ggcagtattc agttcaagat agtccttaat
    23881 cttttcctta ttcttatccg ttatctcttt cttgatgaaa agggagtttt ctctgtaata
    23941 gacttcatca tcaataacgg tataggaaaa gtttttgaca tcatccgttg caggaattga
    24001 agtgatttca tcatctaaaa gctctatttc ttcataagtt gatcctttgg atatttcttc
    24061 acttgctttt gtcagaagct cttttaagtc ggcattttct ttcggcaggc acgcaagtgt
    24121 gtttccaaat cttcctgaaa cttcacgcat tgtgccgagt acctgctcag gatgatctac
    24181 aaaatatttg ttataggtaa gaccattttc atcttcagca aggtgaatcc agtcctcatc
    24241 tctttctctg atactgtccc ttttctttag gaatataata tctgaggtta cttctgttcc
    24301 ggctatgccc ttaaaggtat catttggaag tctgattgcc cctaaaaact ctgctcttgc
    24361 tgcaagatag cgtcttacac tttcatcctt tttatccatt gttccacttg atgtgataaa
    24421 ggcgataata ccgccgtttc tgactttatc aatggatttc gcaaagaaat aatcatggat
    24481 aaggaagtta tttttgttat actccctgtc atttacctta tattctccaa agggtatatt
    24541 gccgataacc gcatcaaaga agttattgga aaaggaggtt tcttccagtc ctttaatctg
    24601 cacttcactt tcagggtata gtagttttcc aattcgaccg cttaccgagt caagttctat
    24661 gccataaaac ttggacttat tcatttcatc agggatattg cctataaagt ttccgatgcc
    24721 cattgatggt tctaaaatgt ttccctgttt aaatcccata tccgaaagcg tcttatatat
    24781 tccgtcaatt acggttttcg gtgtataaaa gctcgttaaa gtagattctc ttgcagcttc
    24841 gtattctgca tgtgataagt tctcatttag aaaagccctt gcttctttcc actgaccttc
    24901 cttgctttca tcaaagactt cggaaagtcc gccccagcct acataccttg ctaaaacttc
    24961 ctgagctgta ctgtcaagct ctctttgtcc gctctccact ctgttaagca tggaaattgc
    25021 ttcaaggtta ttatttaacc tttcgcttgg acttaacttt tcagggagcg tttcttctgt
    25081 aattttaaag ttatgagctt ccgctttcct tatttgagtt tcctcagtag tctgctctgg
    25141 ctgtttatat gtcaggtttt caaataccct ctcaaggtcg ctttccagac gataaggaat
    25201 gacatcgaag cttgttatca tcccgccaaa atactcggtg ttgtccttca ccgttaccgt
    25261 atttaggttc tttctcatat catcaaagcg tgttatggta tggtttttgt ccttgtattt
    25321 aacttcatca ccgacaataa agttcggtct ttcaagactt attttattca gcaagtcctc
    25381 attatctgta aagtctacaa tcggaatttg atggtttcca cttcttaccg gatcaagcca
    25441 caaatcatat cttcctgtga tttcattttt agaaatttct ctgactttat attcttcatg
    25501 attaaaatag acggtatcgc ctgctttaac tgcaaactct tctaaaaggc tgtcctcttt
    25561 ttcattcagt tctacttctt ctctttcttt gaggtaatca aataaggtag cttgaactgt
    25621 gccacgcttt gtagcttctt ctgtttcttc aggtgcttca agctctactt tatcttcaaa
    25681 gctaagttct ccattaaaga caaactgaag ctcctgctca tccatttgtt ttataagctc
    25741 tatatcttta agttctctaa aggtctttgg aaaatcctct aaaatgagcc tctgggcatt
    25801 taattctttc agttcctcaa ggttaaaata tccccattca ggctctattc caagaacaag
    25861 tccaaaggca tcaccgcttt ccctgtcata ctccgtcata taccaagtcc aatttgaacg
    25921 aaaaggaata atatatgctg catgaacctg cttatccgct aaatctacat cctcctgtgc
    25981 gtaaatctct ggcactcttt caagaatttc atcaggcatt agatttccag gatcattttt
    26041 agaataatat tgcggttctt ttacttcttc tatctctgcc gtactttctg tttccttttc
    26101 ctcaaaataa agttttttag aaagcagctc atcaatatgc tttgcaacac ttgtccaagt
    26161 aaggaacaca tcattacaat cattcttctg taatttaaaa ccctttgcat cgtgccattc
    26221 atcacttccc attgcacctg acacaacgtg agaatgtcct ccgattccat attcatcttt
    26281 taggaaattg gctttttcct gcaaagtatg attttcttta aaaaacttgg tgattcgttc
    26341 ttttcctcta tcaacgccgc ttcctcttga aaggctttca aggacttcat cttctgtaat
    26401 aaagagcttt accttcggaa gttctgtcag attggtacta tattcctttc gttgcagctc
    26461 aagttcttgc aacttctgat aaagactatc taccttgtga taatgatacc ttaatacatt
    26521 tctgctttct ttgtatcctt ctaaaaacct gctatattct ttgattgttt ctttcagata
    26581 ttcaggattt tttaatgctt cagataatct tttcgtttct tgcggaaagc caccacctct
    26641 ttcaaaagag ttgaaatatc cttgctctat tccttcttcg cttaaatcgt gcgttagata
    26701 ccataaggat tctgaaattc tatccctttc atagtcctgt gcctctaaaa gttctacatt
    26761 tgtagcaaac tcacctctgt caagaagttc atttatccta cttgctgcat cactccaact
    26821 taaaatttgt gtattatcct ctcttgccga tgttccataa gctaaatgaa tacctttatc
    26881 cgaataccag gaagatactt ctctttcatc aatgtaaaat ccgtttccgc ctttaaaggt
    26941 atctttgagg tattctccca attcttctat gctcttgcct tttgaaaact cagcaataac
    27001 aggaagccta ccgccatcat gatttcctcc gttaataagg acggtatcta tatccttctc
    27061 agttagcgga atagtaaggc ttatctgtcc ataagaattt tcgggtaaag aaaaagaagc
    27121 cttttcagct tctattatcc ctttatccgt attgtctttt aaatctccac tacttccttg
    27181 atggtcattt ctttgagagc tgaaatcatc gccccatact gcggattgtt ctcgtcctct
    27241 atcttccaag ctgctatcaa ctttggcttc tctgctctca taaactctac tgcctgtttc
    27301 tgaatatcca tcaggtgtcc tgtcagcttc ttctccttgt atagatccaa aagcatctcg
    27361 aagtggctct gctcctcgct ctgtgaaagg taattcagcc tcatcactgc gtaggtcgga
    27421 ttcggatact tccttataaa gtccgtctgt tcctccaagc tgtttagcgt attctccctg
    27481 attttttcta ttatctcgtc cgtactctcc atttcggaga actcgctcgt tttcatttct
    27541 ttcctgatca tctcgtcgaa atacattttc ttctacctcc tctaattctt ctttaatttt
    27601 attatatccc gcttcttttc ctattaaaac ttctttttga agctcaagct ctttgctttt
    27661 ttgaatggtt tcatcaataa tctttccgct aatatccgat acactttcac caaggctcat
    27721 taaggatatg ctgtcaaatc tttgaaaatt ttctcttaaa agctcataat ccatcggata
    27781 atctaactta aatcttgaag ctaccgcata gcttaccgag tcccttacaa acttggtaaa
    27841 tgatattcta tcctcatccg ctactctaag ttcattcatt aaggtatcta ttttttcatc
    27901 accgtagagt ctgcttaaag aaaagatgtt ttcaagtgtg ctttcacttt cctcatagcc
    27961 ctcgcttttt atcatttcct ttaagacatc ttgatgggct tctttatcaa atctccaaag
    28021 attgacctcg ttgacatccc tgttttttga aactgtctgg cttatatcaa agatatagtc
    28081 caccttttta tatgtgccgt aatcctctaa aattggtatc cctttctgcc ctcgcataac
    28141 cgttcggtta aagcgttccc tccagtagtc gaacttggca caggctgtcg cttcaggatt
    28201 tttatcatat atacttaatt gacttctaaa atcatacctt tgattgtttc cgacaacttt
    28261 taggagcttt agatattctg cttcattata caaaacatct tgttttatta gctccaaaat
    28321 gttatgaaaa tcatttattc gcatttttac ctccttcttt tttcaaacaa aaaaggcggt
    28381 tagattttac tctaatcacc ttcgctatat accaaatatt attactctat ttcaattttt
    28441 taattgattc actttcagat agtatcttca tttgttcaat agctattgca tttaattttt
    28501 ccaatctttc agattggctc atgccttcat ttataaatac cgcattaaga ttctcaagat
    28561 tagaaaggca gacaagctgt gatatatccg cataatctct aatattgcct tttaaatccg
    28621 gattttcatc acgccactcc ttagctgtta ttccaaatag agaaacatta agaatatccg
    28681 cttcacttgc ataaataaaa ttgatttttt cttttgaaag tttatccggt acaagtttgt
    28741 ttttaatggc atctgtgtga attctataat tgatttttgc caaatttctc ttaatatccc
    28801 atcctaactt cttttgttct tcttccttta ggcgttcaaa ttccttgatt aaatataatt
    28861 taaagtatgg cgatacccaa gaagcaaact caaacgctat atctttatga gcataagttc
    28921 caccatatct tcctgctctc gcaacgatac cgatagaatt agttttttcc acccattgct
    28981 tgaccgataa tataaaacgg ttaaatcctg cttcattttt aattccctcg aattcggggg
    29041 aattaaaatt cgtgttatac atttcttccc aaatacctaa aaactctatt gtattcttgt
    29101 ttctgagcca cttttcaatc aaagcagaac cgttctcaat atttctaacc atatccgtta
    29161 aagaaatata atctctattg tctatggcaa taatgctgat ttctgaacct tcaacattta
    29221 ttttagacat ataatcacct ctcaatcatt aaattgatta ctttgcatta tagcattttt
    29281 agagcatttt ttccacgctg aaattagtgc tgtcttactt gcgatatgtc aaaaccgctt
    29341 cttttatctc attttcaatc gcatctaaaa attctttctt agaggattta ccctgagcaa
    29401 tatcagataa ttccatttcc cactttgctg tcgtttctgc cgacttaaag gtatctgcta
    29461 caatcgttac aaggctgatt cctttatgcg ttgcaattag attcttctta tctctttcga
    29521 caaatctctt aaagattaga ttttcaataa ttcctgccct tgttgccgga gtgccaagac
    29581 cttttctttc cacctctaca cctttttcta aggcatcatt tcccgcaatc tccatagact
    29641 ttaagagcgt atcttcagta aagtgtttcg gaggttgggt aaatttttct ttaacctctt
    29701 tattttcaac gctcaaaaca tcaccaacac ttacatcggg caatacagca tcttcactct
    29761 tttttgattt gtattcttta aggtatttgc taaagccttc atccttaatt accttacctg
    29821 aacttgtaaa ttcaaaaccg tcaaatgaag ctacaatctt tgttgtattt tcaataagcg
    29881 gatagcctac acttgcatga aatttatcgg atataaggaa gtaaactttt gcttcactta
    29941 aaggtaattc agaaacatct tcttttactg aactgattgt cggtatgatt gcatgatggt
    30001 ccgtaacctt tttagagtta aatactacct tgatacgctc tgtatcaaag tcatttttac
    30061 ccaaaatgtt gttgactgta ctcacaatca tatcctctgt taaacatctg ctgtctgttc
    30121 tggggtaagt aatcagtttc ttttcataca ggctttgagt atagtcaagt gtctgcttgg
    30181 cactatatcc aaaatattta ttgcactccc tttgaagcgt agttaggtca aaaggtaaat
    30241 caggctttgt aattttttct ttttgaataa catcagtaat ttcaatctta tcgcctacta
    30301 aacttataag ctgttcagca gcaacttcat catcaattct gtctgtggag agtgtaaatc
    30361 cattcataga aagctctact gtgtaatatt tttctttctg ataatttgct atctcatcat
    30421 ctctttttac aatcatggca agggtaggtg tctgcactct gccaacactg taattttgct
    30481 gatacagaca agagtagagc ctactaatat tcattccgac taaccaatcc gcaatagctc
    30541 ttgcctgtgc cgattcaaag agattatcat agtcttttcc gtctgttagg ttatcaaaac
    30601 cctctttaat ggcactatct tccattgaag aaatccaaag gcgtttcatc ttctttttac
    30661 aattcacttg attatataca agtctaaaaa tactttctcc ctcacgccct gcatcgcacg
    30721 cattgataac cgtatcaacc tccttatcat tcatcagctt tttaaggatg ttaaactgct
    30781 tcttggttgc ctttgctact tcatacttgt agtcgctcgg aataatcggt aaatcagcca
    30841 tattccactt ggcgtacttt tcatcataag catccggatt tgccatttga attaggtgtc
    30901 caacgcacca gctcacccta tatccgtttc cttcataata tccgtctttc tttttatttg
    30961 ctccaataac ctttgcaatg gagattgcaa cactcggctt ttctgcaata actaacttca
    31021 tctgtgtttt cctcctgtta taaatcaaaa tagggcgaaa gatgttactc tcccgcccta
    31081 actttgcacc caaaataata tgtttttagg tgcgtttatt cttcatcttc tacttcatct
    31141 gcatcattta tttcttcctc gctttcagat tcagaaaaga aatcatcatc ttcctcctct
    31201 aaggcttcaa gttctttatc ttctttcttt ttcacaacct taaaatagta gcctgctcct
    31261 aatgcaccac ctactacaag aagcaaaata atataggttc ctaaattact cttttcgtcc
    31321 ttcttctcag gctttacttc ttccttaaca ggttcttctt ttactacttc ctgctttggt
    31381 gcttcttttt tctccaccat gttaagcaaa tcatcttcag atacttccgt aagaagcatt
    31441 acattctcac tatcttcatc atgattgatg attaaatgaa aagtcttacc gtttttcgtt
    31501 tgaaaggtaa taaactgcct tgcatctgcc gaatactgat ccgtttcttt gttatcgctg
    31561 ctatctccat gatgaatcgg atactcctta tttgcattat ctttattctc cgtaacggat
    31621 gctctcgcct ttgacggaga tgaagctact cccttgttgg tgtttacact cttacttgca
    31681 gaccccattg atgaatcctg actgttattc gcaggagctt ttgccgtcaa cttattggga
    31741 tagcgaactt ccttttcttt ctcctttact tccgttttgc ctttaccggt atcctttgta
    31801 gtattacctg agcttaccga atccgatgag cctttaccgg tactacttac agaagtctgt
    31861 ggtgtttgag gagaaatacc tgaacttgtc ttaattccgg aaataggact aatcggtgta
    31921 actgattgtg atgttacagg agctttattt gtttcagttg cctttttctc aagctctttc
    31981 accttttccg tcagcttatc tatttccttt ttcatatctt ctgataagtc cttatttccc
    32041 ttatctttct tcattttttc tttcaggcta tcaatttcat cttcaaggtc tttaatttta
    32101 ttcttttgct tatcacttaa tttgtctttg tccttaagtt cgcaatttaa cttatcaagt
    32161 ttatcctgaa gctcttttgc ctctttttct atttttgaaa tatcgtcttt agaaagctct
    32221 gtctgcgttc ccttatcttc tgtctgagtg cattcatcct gctttggctt ttcttcttcc
    32281 gtctgcgttt ccttatcctt aacctccatc tttgctacct tacggataat ctcatctttt
    32341 ccatctccct ttacctcata aaacagaaat tcatcaatga atttcatatc atctgggagc
    32401 attggaagat ctccgctatc aataagttgt cctttctcag cctttatctt ttcttcctta
    32461 aagacctttt catcttcaaa gatatacctt acatttactt caacttccgt ttgaacagct
    32521 tcgttttttg ttggagcttc agatttttgc tcctgtgcat aaacaaccgt ccaaagacct
    32581 aaaatacaac taatacttac aattaccgca agtgctaccg tccaaaactt attattcttt
    32641 ttcaaactgg ttttcatttg ttttctcctt tttcatttct gctttttgtc tgtttacctt
    32701 tgctatcaaa tcgctgattg tgatgttgtt ctttctgcaa atggcaatga tttcttcatt
    32761 ttcaagctct tcttcacgaa tgaataaagg ctccaattct tcatcaatca gagccttttt
    32821 atcctttaac ttctttattc tgttttttac tgttgtaagt tcctttttca aattgttacc
    32881 tcctatttct ttacatttgg cggaaaaccg aatcctacgg gatggtgctt acaaaatgtt
    32941 gctatccaat catctaaggt agttactctt attcgaccga tgttatcaat cacttttcca
    33001 tctccaatgt aaatacctac atgaccatag gtaagccctg cactgcttcc gctactgctg
    33061 ctttcaactg ccacaagcat tcccactttt agctttgacc tgtctgatgt aaaggtatag
    33121 tttcggtaca tatcacaggc attcccgcct atatatccaa gtcctgcatt ttgatagacc
    33181 tgtgataccc acatggcaca ccagcctgca cccggagatg gcgtaatgta tgcagcatta
    33241 acaatcttct tttgtacttc cgtagatgcc tcatactctt ttccaccgcc gatacctcca
    33301 tttgcactga taaggtcaga attaccaaag gcttctctca tattgccttg tgcaaggaac
    33361 aaggcttcat agtgctttag attatcggga tagtttgcaa agacctttcg gataatactg
    33421 tccatctctt ttttatgaag cgttacgatg agttttttat actcataagg ttcttcgtgg
    33481 ctttcggtat attcattgcc atcttcatcg gtataggttt ccgtaaccgt cctgtatctg
    33541 atttcaattt cttccctata ctcaaggtca tacatggact caaataattc ttttaatatg
    33601 gattctactt ccgatacact ctttacctct ccgcaccttg atgtaatgta ggataaaagc
    33661 tcatggacat tatgaccgat gtattctgta ttatttaaga tatattcatc atatccagga
    33721 taattttctt tcacatagtc aacttcactt tgaagctcac cctccatatc tgaaaatttc
    33781 tgattgattt cacttaggac atttggcttt gataaatagc ttgttgtaag cacagagctt
    33841 gtagagttca taaagcctgt cattcccgtt cctgcaaagt taatcacaaa agtccctaag
    33901 ataatgatac ctatgaaaat aagcattagt ccttttgctt ttcggataat catatccttt
    33961 gagcttttca gtgttccgat aagtccctct ttaattcgat ctctaagcct tgacttattc
    34021 tcacgagaaa ttgctgcctt tacctgattt ttcttttgaa atcttttata tgcacttgct
    34081 cttttatact catccgtctt tttcagttcc tttttggcat cacgaaattc caactttgac
    34141 ttacgctttc tgattttata gtccttattt gtaaggtcat agcctctttt agctcttttc
    34201 ttatcagagt acttctttat gccatgaatg agcttggagc ttgtatctgc tgtcttttct
    34261 cctgcttcca ctccttgatt ttcatcactt ccatgagaca agtaatctct tacagtttca
    34321 cttcctttag caagtcctga aagggcagat acctttacca tgtctttttt tagctttttc
    34381 ttttggtctt ttgataggct gctataaact tttccgctga cagcatcttt tcccgccttt
    34441 ttatcatcag ccttatttcc ctttggtgca tcgtcctttt tccttgtata aaggctttca
    34501 gaatagttct tcctcttgta cctgcttttt tgagtcttat gatttttaaa ggaattttct
    34561 aatgattttt ctttatggga aagattatcc tccacatcat aggtcgattc aaaatagttg
    34621 ctgtctctaa agtcattatc gtatctgtct ataatcccgt cattatcaag gtcttttcct
    34681 aaaggatcat agatttttcc atccttaacc tccgtaatat aatctgatct tcccgtttca
    34741 attgcggaat ttacgtctcc cttatctgaa gttctatatt tttcgtttct cttggatgtt
    34801 ccataagact tttcattatc agcacttacc ttacttgttt tttcatgcac tttatcctga
    34861 aaccgttctt tctcatgaac gattttacct ctgtaatcat cgttatgttt tagcttactt
    34921 tcttcaggta tcgtatctgt tttactatga aacatctctc tttcaaggct tgccttatgc
    34981 ctttccttaa aatccttttt cagcttcttt cccataggct acctcacttc ttccggcttg
    35041 gtagtcatct tctgatataa aattgtatct ttcggaaact tatcaatgaa aggcacaatg
    35101 gtatttccaa agaataagag tccctcacct tcatttgaat tagtaacata tctgagctgt
    35161 ggaagtgaga ttttaagttt tcttgcaagt atctccctat ctcctgatgc ttgattaagc
    35221 attaagacaa agtctgtatt gtcaaagata ttttctatct ccttactcat aagtaggtct
    35281 ttgacattct gcgtaatacc tgttggaata cctccccatt tacgaaatct tttccaaatc
    35341 tctacggaat aggatgctgt ctgctcatct tttaaaagca aatggaactc gtcgatatag
    35401 taccttgtag ccttgtttcc tctgttttgt gacaccttat tccacacctg atcctgaata
    35461 acaagcatac cgattttctt taattgactt ccaagctcct taatatcaaa acaaaggagc
    35521 ttcttattta aatccacatt tgacctgtga ttaaagacat tgagagaccc cgatacatag
    35581 atttccatct ctgttgccag cttcttaccg accttttctt cctgcccttt taacatatcg
    35641 tataggtctt gaagtatcgg catattgtca ggagtaggat gttcaaaata cttttcatag
    35701 attttaggaa ggcacctatc tataacggac ttttcttctg ctgtaagacc actaccgcct
    35761 actacaagct caagcatact catgataaag tttgccttgt ctttcagcgg tgcatcccca
    35821 tcaccgtagt tcatgtttat atcaagagga ttcaggtaat ccttcgactt actgctgacc
    35881 ttaatgactt ctcccttaaa ctgccttacg aggtttccat actctccttc cggatcgcag
    35941 ataatcacat catcatctgt tactaaaatc gcatttgcca tttctctttt ggcactaaag
    36001 gacttaccgc tgcccggagt tccgaggatt agaccgtttg ggttctttaa tttctttcta
    36061 tctgccataa tcaggttatg gcttagggca tttagtccgt agtaaagact attacttgaa
    36121 ttgataaaaa gctcctctgt ggtaaatggc ataaatactg ccgttgatga gcttgtcaat
    36181 cctctatcta tttcaatctt atttacacca agaggcagca cgctcactaa accttgttcc
    36241 tgagaatggt caagtctttt cagcttacag ttatgcttat ttgcaatgga gcttatttga
    36301 gcaattgtgt tatcgagctt ttgcactgtc cttgcaaagt tcataaagac gatacttacc
    36361 acaaacattc gctcatctct tgtctgcaag tcttttaaga ggctctttac atcctcacca
    36421 taggtaatta agtccgatgg aagaatgtcc atatcataac cgcttcttac cgccttctta
    36481 ttttcctcaa ttttcatctt atcaatatca gtgttttttc gttttaccat cttaatggct
    36541 tccgattggt cgattgccct aatatgaaag gaaatattga tgttatcgtc aatatccaaa
    36601 aactcggata acatcctatc tgaaagctca ctggcaagga tttgaaaatg acttgttgct
    36661 ccgatgaatt tgccgaactt aaagtatcta ctcggtgtaa agttaaactc atcaggcaca
    36721 atatatgtct ttgtgctttc ccttttcttt aggtctttgt aggaaaattt aaatgtttta
    36781 tttggattta aaacatcatg aagtatcttt agcctttctt ctccgtttag gctttctgct
    36841 cttactccca tacttttaag actcgataat atatctatct ccagtctttc aagttttgat
    36901 gttgcctgtt ctaaattatc cgcttccact gtaaaggtta catactttga ttttttcaat
    36961 ccgttatttc cctttacaat ctggctttta agcatctctc taaattcaaa gcgtatatcg
    37021 tcgaaaccgt cctttttatc cggtatctga attgccgact tcatttcttc gtttcgtccg
    37081 agctgattga tatatgaaaa ctcaatgctg atacttggat caaaagagtt taggaagttt
    37141 gcaaattgat taaaaatcaa gtccctatct tcatctaagg caagctgata gttaatgtcc
    37201 tgaaaggcta tacttttact gaagtgcttt tcatcaagct ggcatattcc gctttttaaa
    37261 agccttagat agggaatggt atcttctacc gtatatcttt tcggttcttt cttaaagata
    37321 aggtcaagga ttcctccctt atcttttttt gacttgcaat tacttttctt taagtcccgc
    37381 ttttgacttc tcaatgcctt ttcgttttgc atgagtttta tttgctgaat ttttcttttc
    37441 ttgttcaagg taaacctcct ttctcactct cttttgcggt tgataaaact tatgaagata
    37501 aatgtactta aagtatttct caaatgtcag tccgtctttt tcaaatagtg tgatgaaaaa
    37561 gataggaagc gtggatacga taaggaatat gacagctatg tcatttggca caaacttcct
    37621 cataaataaa tagactggta ttccaaccag tcctgcaagt gtgaagccta tgagctgcct
    37681 tttcgttagg ttaaaagcaa cctttgtctt taccctattt aagtctttag ggattggtac
    37741 atatgccata tcttacctcc catcttcttt accctttgac agctcctcaa agtgttcata
    37801 tacagagccg atttcttcct gaatagtcgc aagctgttcg tctgtgttct tgttatatct
    37861 ttgctgtatt aggtaaaaat cgttatggca gcgaccaatg tcctctgttt tttcttccaa
    37921 ttcttccacc ttattatgaa gcctgatcct atcaattatc gccataatac ctgctccaat
    37981 tactactata aatactgttt ttcttttgtt catattaccc ttcctttctt ttagtgtgca
    38041 tttaatacgc ttttggccag tgttccgctc tttaacatca ttaaccctag caaaaccgca
    38101 tatccaagta tcgtaaaggt acttgtgtgt atatctgtta tctgtattgt cttaactaat
    38161 actgcgtata ttccaagaca aaccattaaa aagagtcctt gtaaacctat ggcaaacaat
    38221 cctttgatat agtttgttcc aatctgtccc cactctttgt ttcccattgt ggcaaatgga
    38281 atggctgaaa tggatgagta aacataaatc tcaaacattc ttccgtaaac cacaagcata
    38341 atcacaattg aaatgacctg tattgccacc tttataagcg aggtttcaaa gagtatcatg
    38401 acaagctctc caagaccttt atcctttaat ccatctacca tttttactat ctgatctccg
    38461 gagataacgg cagaagtatt gattacccct gccgctttat ttaccatgtg ctgtgccaca
    38521 tcaaagaccg ccattgaaaa ctcaaatgca tgtgatacta accatacagc aatccacatc
    38581 ttgatgatgt acttgaaaaa ttcaaaagta tctgtatcgt gcatattgtt cttttgcatt
    38641 accatattga taagctcgat acaaaggact gctgtgatga ttagccctgc tatgggaata
    38701 atgacggaat cgttaatgct tttaataaag gcaaatacat ctccgttcca ccccatagga
    38761 gtttttccta catctgttgc aactgcacca actttatcgt tgatgtcaag aaacatggac
    38821 tctaagtttg cttgaatacc gcccagtaga agctctttaa agaattcttc tatcttgtcg
    38881 aatattccaa acatctaagc tctccttttc ttattacttg agtacatttg caagaagcgg
    38941 aatcagctta agtccgataa ggacaatgcc tcctcccgcc ataagctgct tgatgccctg
    39001 agatttcgct cccggattat cattaccata tccttccata aggttaataa ctccccatgc
    39061 tccaagtcca gcacctactg ccataactaa aatctttaat acatttactg cctgtgcgaa
    39121 aaattccata atttattcct cctcattttc ttctttcttc tcaatcttgt tatatgactt
    39181 cactacaaag tttttgtaag tctttccctc tttttcacgc ttcttaaagt agccgaatac
    39241 atgaatcaaa tcgccttttt caaagtcctc tgctttctct gctttttctc cgtaggctgc
    39301 acaattgatg tactccttgc cctttccata tttttttaca agcgtaaagt ttacaacttc
    39361 tacttcttca ccctcttttt caaaacttga gaaagtaggt tctgccacta agttggcgtt
    39421 gatgttaatc atttcttgtt tcatcttttt aaatttctcc ttttcatttc aataaaaaaa
    39481 acgactggag tttttctttt ccaatcgctg atactcttac tatttagttt tcgtttaagt
    39541 gggatttctg atccttatca tcttgcctcc tgattttgaa taaaaaaaca gcaaatcttt
    39601 ttaagactta ctgcattctt gtaataaccg tctctctatt taattttgct tttccttttc
    39661 gcttcatata gctttctatg tcaaacaaat tcttcttgtc ataatcctca agcagcttat
    39721 aattcttgtg ctttgtaata tcgtatttat ctgaaagaaa aggtctgaca cctctaagct
    39781 gaaatataca ttttccaccg tccattacag taatctcatc ttgactcatc agttctttgc
    39841 cacaatgtca agtgatgaat taaaaaaagc tatcaaagaa atccttcttc gatagcagcg
    39901 tattcaacat ctattcacat ttcctgcctt gcgttcttcg agctgatgaa atataataag
    39961 aaatgttgtc ggaggtgcga atatggacta catgacgctc aaagaggcaa gcgaaaaatg
    40021 gggcgtttcg tcccgacaga taaattatta ctgcaccgat ggacgcatcc ccggtgctgt
    40081 caaaatggcc ggtgtctggc tgatccccaa ggaggcagag aagccaaagg acagacggta
    40141 taacgaaagc aggtgataca gtgctgcata tagcaatttg tgatgatgaa aaagactttg
    40201 ttcagtattt gaccgattta ctgaaccaat acagcaagga aacaggacgg gacataaaga
    40261 ttaccccgta ctatgacggt atggagctga ttgaaaaata cgatccaacc atagacctga
    40321 ttttccttga tattcaaatg aaggtggtgg acggacttca tgctgccgag cgtatccggc
    40381 aaatggacga aaccgtgggc attatttttc tcactactct tacacagtat gggcttgagg
    40441 gatataaata ccatgcgacg aactacatca ttaagcccat gaaatatgcc cggctcaaat
    40501 cagaaatgga caaattcttg gagcgcagcc agaaagaaga taccccctct ctggtgatta
    40561 ccaacgatac cggcaaatat aaggtgccgc taaaatcaat tcgatatgtt gaaacatata
    40621 accgcaatct gatgttccat accgagcagg aaaatattat ctgctataag agcatgaaag
    40681 aagtggaacg ggagctttgc gacaaagact ttgtgcgctg ccataccagc tacattgtca
    40741 atctgttcta tgtgaaaggc atcaagaagc tggacattga attgattaca ggcgagatca
    40801 ttcccatcag ccagccgaaa cggaaagaat ttatggaacg gctgacggac tattgggggg
    40861 atatgctgta atgtggttat atcaagtgct cgacataaca gcaacgctga cagaatcttt
    40921 gggactgtat ttattcagta tttgtttttg caaagaacca cggtatcgtg cttctgtaaa
    40981 tagatggctt ataacaggta taagtttttt gtgtgcgtat acattgacat ggttttctga
    41041 acttggcgca ctcaaagcgc cttttcttct tacgatttat cttgtccttt tgaaagtttg
    41101 ttatagagat tctgtctatc aatgtgctgt tgctgaagaa ctgaattata ttgcaacttg
    41161 ttctcgatat tcgcaaggta gcgcggggaa atatataact cgtcgcttac ctttttgcgg
    41221 ctctcgccgt attcatttct cgcggctttt atagctgccc cgaaagcctt aaagtcgtac
    41281 aatggtacgg gtctttttgc cattttcatt caccctgtta cattttacag ttcacctttc
    41341 tttttggata tgtccacaca attcatatca ttaggttaaa tacttcctgt tgtgtattga
    41401 cagtagactg attttctgat aaaataaaat ttatgtaaaa ggaggcggcg tttatgttgc
    41461 atagcatgcg tattagataa gcattgaaaa aaaggatttt cccattttca acatgggctt
    41521 ttttgtcatg cttattttgc ggatgctata caaaaaacta acgacgtata gatatagtat
    41581 agacatagtg caagctatgc cttagttttg ttgtactgct ctgtgcattt tttgggtagg
    41641 gaaaccaccg gctgtgccgg tggaggtacc ccgtaaaaaa gctttagctt caagcatcga
    41701 gcgaagcgag ctgctaacag caaattatca gtgaagaaag acttgcatta gaacagcggc
    41761 tgcccgttta cgggctgcgg ggcaaatgat gcgggtgaaa taaaattcag tgcgtcttga
    41821 gacgcatgcc ggtaataggc ccttataggg ccaactcaag ccaccggctt agccggtggt
    41881 tatgacttgt gtaaaaagga cgagtgaaat ataatgctta aaaaatattg gataaaatgt
    41941 ccgatttgta acggaaagac gagagttcaa gtatttcata atactgtatt aaaagatttt
    42001 cctcttttct gccctaaatg caaattgacg catatcattg atgtagaaaa attagagatt
    42061 gtaatcaaaa atacagaaaa acaaactttt attatttaga agaaaggata taaaaatgaa
    42121 acgtttacct aaatatacgc ctgcggaagt acggaatgat ccatacggat ttacttacaa
    42181 agaaatgtcg gaagttattg gcgagaatga agcaaaagcc ttatatgaag aattatataa
    42241 gcaattacca cgcaaaaaaa atctatcaat gttggtaaaa aatatttgca aaagcagtga
    42301 tactgaaaag tatgtttacg aactgaaaga caacaaatac attgaaacgg tttttatcaa
    42361 gcggcgagat ggtggaactg tttgcgtgag cacacaagtc ggttgtcctg ttggttgtat
    42421 tttttgtgag tccgggcgaa atggctttgt tcgtaatcta acatcgtcag aaatcgtaca
    42481 acagattata ttgttgcgtc gaaaagtaaa ccgtatcgtt tttatgggta tgggagagcc
    42541 tttattcaat tatgacaact tgataaaagc aatccatatt ctccgagata gatatgggct
    42601 caactttcca accgacggca ttaccatatc aacagttggt ccggtcgatc aattaaaaaa
    42661 attgcgcgag gaacatctta aaattcagtt gacaatatct ttacacgcag caacacaatc
    42721 tgcaagaaat cgtattattc ctcacatgcg catatatgct attgaagatg ttgttaagca
    42781 agccttatcc tattctgaaa ggcataatcg caaaattgtc tttgcgtatt tgcttttacc
    42841 gggtataaat gaccggccct cagatgtaag acaacttgca aaatggtttc ggggcaaaaa
    42901 agttatgatt aacgtgttac aatacaaccc aacaagcaat tcaagaatta aagcaccaca
    42961 gaaacgggaa atagttgcat tcaaacatca attagagcaa gcaggacttg aagttactat
    43021 gagagtttct catggcagag agattaacgc ggcttgtgga cagttagcta acacatataa
    43081 taaattcaaa aaaaaatgat taaaagtgcc agacgcatag acgcagagcc gataacgcat
    43141 taggtcaaaa aacctatgtg ttatcggctt ttttatttaa tattgcgcaa aagccgctgt
    43201 tccctattat agaatggatt gcgggtagtg tattaaagtc gccctttttt aaggatacgg
    43261 cggtatcggg gaggtatcgc cgtgtccatt tttatttact gtaacccgcc taatgctttg
    43321 cggtcaaaaa aagctatgct ccgcaagcgg gatattccgg ctatgaatgt gaactgtcaa
    43381 tacatttagc tactgcttac atttcctttg cgcccgtccg cgtcttgcgg acgggcgcat
    43441 tttgcttttt tcaacttttt tctgaaaagc gcactcaaga gccatctccc gaacgggtac
    43501 ggagcgaaag gggcagagag gacaagccct taactcccgt cctctactcc acgcgggaag
    43561 gaggtgaact ctatggagct atcttcttcc gacaaggaaa gaatacaaca tcagtacgac
    43621 gcattagcaa agaaaacttt ggtcggcgaa gcgaaaagcc accgccgcac tcttgcgaaa
    43681 cgcgcagcac gcgaagttac tttttcggat ttgagcgaaa gcgaactcgc gcagcttttc
    43741 acaacggacg aatacgaaag cgattatttc cgttttcaag tgtccggctt tgatgtactc
    43801 gtcaaaaatg aactgcttgc cgaagccctt aacgctttgc ccgaaaggaa acgcgacatt
    43861 atccttttgt cctacttctt ggatatgagc gacgcggaaa ttggcgaact gctgaatgtt
    43921 gtacgcacga cggttttccg gcacaggaaa tccgcgcttg cgaaaatcaa acagtatttg
    43981 gagggaaaag cagatgatga ataccgttag gaagtctgaa aatctgttgc cgttccctgt
    44041 catttccgca gcggcaaacg gcgacacaac cgccatgtgc gcgatcttga agcactacga
    44101 gggttacata gcgaaacttt gtacccgcac gctgaaagac gacgcgggca atacctattc
    44161 ctatgtggac gaggaaatgc gtaacaggct gcaagtgcgc cttattaccc gcacccttgc
    44221 ttttcatgta ggataatctt ttagcccatg cggggagcgt gtcccctttc cacgcttccc
    44281 gttatgggct atttgtcgtt ccgcaaaagc atatccgctg ttgatgtgct tttgcaggcc
    44341 gacaaagcct attgttcttt gacaaagaaa gcggcctttg gtgcgcagca taacaggcaa
    44401 acgggtacat tccgtctgta ccgagccagt cggcgggtac gccatgacag cttttcccca
    44461 taggggaaaa gccgagcgag aactaccgcg ccgtaaaaca ggtttagcag cttgtgggcg
    44521 acgacataca aggcggcaca atgatactcc cgcgttgaac accctcaaag tattgcgcgg
    44581 cgcacccata agcatgggcc ggggtgaaac tcccgtgagg ttgcagctaa caaccgcccg
    44641 tttttgcctt ttgacgaata tagtttatcc atacaggaaa aggaggtttt tctaatggat
    44701 aaaaaacaga caattacaac cgaacgcaaa atagggaaaa tcacctatct tgttcaagcg
    44761 ttgccgagtg aaaaggcaac cgatacaatc cataaaaaga ttgagaaact cattgtaaag
    44821 gatttgcaga aaaagcccgg aaatccggga tttttagcgt ccgagtagtg cattaggcgg
    44881 cgggatatgg tataatgata gcaacacatt atacctgttt attcggctgt cggaaaggag
    44941 gactaatgtt acagtcgaat aaaatcaccg ccctttactg ccgtttaagt caagaggata
    45001 tgcaagccgg agaaagcgga agcatacagc accaaaaaat gatacttcaa cgctatgcgg
    45061 acgaacacca ttttttgaac acaaagtttt ttgtggacga cggattttcc ggcgtgagtt
    45121 ttgagcgcga ggggctgcaa gcgatgttgc aggaagtgga agccggacga gtggcgacgg
    45181 tcattaccaa agacctttcc cgtcttggca gaaactatct gaaaacgggc gaactcatag
    45241 agattgtatt tcccgaaaac ggagtacgct atatcgcgat caacgacgga gttgacacag
    45301 cgcgggagga taacgagttt acccccttgc ggaactggtt taacgagttt tacgcccgcg
    45361 acacaagcaa gaaaatccgc gcagttaaac aggcacaggc gcaaaaaggc gagcgcgtca
    45421 acggggaata tccatacggc tatatcccag acccgaacaa ccgccaccac cttatacccg
    45481 acccggaaac cgcgccgatt gtcaaacagg ttttcgctat gtttgttagc ggcgtgcgta
    45541 tgtgcgaaat ccaaaaatgg cttgcggaaa acaaagtctt gacgattgga gcgttgcgct
    45601 atcagcgtac aggacaggcg cggtatcagc gggcaatgat cgccccctat acttggccgg
    45661 acaaaacgct ctatgacata ttagcaaggc aggaatattt agggcatacc ataaccgcga
    45721 aaactcacaa ggtatcctac aagtcgaaaa agacccggaa gaacgaagaa gaacaacgct
    45781 atttcttccc aaacacccat gagccgcttg ttgacgagga aacctttgaa cttgcgcaaa
    45841 agcggattgc tacccgccac cgcccgacaa aagcagcgga gattgatatt ttttccggct
    45901 tgctgttttg cgctggttgc agacataaga tgtattacca acagggcgta aatatcgagc
    45961 cgcgcaagtt ttcctattct tgcggcgcat ggcgcaacag ggcaaggaca ggcagcgagt
    46021 gtacctctca ttatatccgc aaaaacgtac ttcttgattt agtgctggaa gatatgcggc
    46081 gggttttgcg gtatgttaag gaacacgaac aggactttat ctgtaaagct accgagtacg
    46141 gcgacatgga agcgagaaag gcattagcgc agcagcaaaa ggaacttttc aaagcacagg
    46201 cgcgtatgac cgaacttgac acgcttttcc gcaagctgta tgaggacaac gcattaggca
    46261 gactgacaga tgaacggttt gtgtttctaa cttccggcta tgtggacgaa aagaaatccc
    46321 ttgccgcaag gatagacgag ttacaacagc agatcgcaac cgttaccgag cgaaaaaggg
    46381 atatatcaag gtttattcag attgtcggga aatacagcga catacaggaa ttgacctatg
    46441 aaaacgtcca tgagtttatc gaccgtattt tgatacatga attagaccgg gaaaccaaca
    46501 cccggaaaat cgaaatccat tatagctttg tcggacaggt tgataccgag caggagccga
    46561 cgcaagttgt caaccatgac cgccgcaaca tggtagatgt aaaaagtatc gctatctaac
    46621 cccagcaaaa aaggggcctg aaatgaaaga gtacacgcta attatcaagg gagaaatgga
    46681 ttttatcatc ttatctccgc aggtgctttc ttccctgatt actcaaatcc ataacagccc
    46741 ggagcgaaaa gttgtggtaa gtattgagag catcatgcca ccaaagttta ccgactatct
    46801 gcttcgggta atcaatagca accgctttag caatgaacgg ttccgctatc gctacatttt
    46861 agaaaatcct gttacaaaga aagggatgta tgagatttta cggcaacagc tttcccgcac
    46921 caatacggag cgatttcctt gttttcagac catacaattg accgacactt tccagggtaa
    46981 tgtggagctg gacatggagt gcaacgatct gttcttttgg gcttgcaaag atacagcagc
    47041 aaagtttgtt tacacgttcc cggatggacg ggaggaaaca ctggtgattg aatactgaat
    47101 gagaaaagcc ccatccctca atggttgaga gatggggcaa actatttacg ctgcaatctc
    47161 cgggatttcc ccgacaaagt tataaacaat tctgacttcc tgcaccttct gaccgtccac
    47221 cttcttaacc tcgccgatca ggattttgct gataagccgg ttcagtacgg actcgtccag
    47281 ttccgcaatg gcggcatagc gccggatttc cttgataaag gttcggactt cgctttcctg
    47341 tgcgtccgcc cggctctgca tcaccgccag atcgtgcagg cgtttctgat tggcttcctg
    47401 ctcctgttcc agcgccgccg tcagcttcat aaaccgctgc tcggtgagga cgcctttggc
    47461 cttatcggtg tacaggctca aaaacatccc gtcaatttcc tgattccggt cttccagccg
    47521 tttccgttcc ttctctgtct gggaagcgtc caccagatac cggcgctcca tccggctgct
    47581 gagccgctgg tagaacgcat cggcgtcctt catggcctgc gccgccaatt cctgtatatc
    47641 cttcagcacc agattgtaca aatcccttgc ctcaatcttg tggctggtac aggcgttctt
    47701 gcccagccgg ttgtaagtct ggcagatata gtatgccctg tcaatcggct cccgcatttc
    47761 gccggtgaag cggttcttcc cggttctgcc gaccttttca taccgcacct gcatggactt
    47821 cccgcaggtg gcgcagtaga caagcccgtg gaacaggtta tagaaggggc aggcgttgcc
    47881 tttcataatg gttggcctgc ggtcaatgat gtcctgtacc tgttcccatt cctccggcgt
    47941 cacaatcgct tcgtggcagc cctcgatgac ctcccaatcc tcacggggga taatgtcata
    48001 agtgttggag cggatgcctt tctggtgtgt ccggcagaca agatgtgcgc ctttataaaa
    48061 gggatttctc aggatatggc tgatccttgc gctcccccat gaatagtagt tcacgtcaca
    48121 ttctgtattg ctttttactc tggtgatggg gattttatca tccatgagct gctttgcgat
    48181 ccgcatacag ccccagccgt ccagtgccat atcatagatt tttcggatca ccggcgcagt
    48241 ctccgggtca aggatcagat gccctttgtc ctccgggtca cgcatcaggc caagaggcgg
    48301 ctgcccgccg cagaactttc cctgccggga acgggtcatg cgtcccgcca gcaccttttt
    48361 ggaaatatcc cggctgtaca tatcgttcag gatattctta aagggcgtga tgtccatttc
    48421 ctgacgggtc aggctgtcca caccgtccgt gacggcgata tagcgtacat tgtgtttggg
    48481 gaaaaagatt tcgatatagc tgccagcttc gatataattc ctaccgagcc tcgataggtc
    48541 tttcgtgatg acgcagccga ttttccccgc ctcaatgtca gcgatcatgc gctggaacga
    48601 ggggcggttg aaattgcgac ccgtatagcc gtcatccaca taatactcat actggaacat
    48661 cccatgcttt tctgcgaagt cccggagcat gagtttctgg ttgctgatgc tcatgctctc
    48721 attgtcgccg ccgtcatcca gagaaatacg gcagtagaga gcggtgattt tttcatagac
    48781 ttgttttttc ctgctcatag cgttctcctt ctatgaacag ggacacgata ccattatagt
    48841 accatgtccc cgtccctgaa tcaaccgttt ttacgcagct ttttctgcca tttcccgctg
    48901 atgacgccat tcctcgaagt gttcgcaggt ctggcgctca atgagctgct caaaggcttc
    48961 ccgcatggac ttctccccgg agaactcccg caccaccaca aaacggactt tattcttctg
    49021 atttttgctt ttttccatag gctacctcca tagagagaca ggccgaacgg cagcaccggc
    49081 aacgaagtcc ggcaacgaac ctctaaaaac ctgtaaacta ccgtctggcc tgcaaatctt
    49141 actttgtgaa ttttcttgaa aaatctcgtt gctttcgttg ccggagataa tgataaatat
    49201 taaaatatcc cttatttact ggctttttcc gtatccagac agggaaatcc ccggcaacga
    49261 acccggcaac caagtggcaa ccatcccggc aacagaccgc cattttcagg cgatccactc
    49321 cttcggaagc tccaactggc ggcattcttc ctcgctcagt tccacaaatc catcttcgtt
    49381 gccgggcagt tcgttgccgg aaacgtcccg ttcccagccc ttctgcctgc cgtatccggc
    49441 gaacatccgg ggattggaga atggcttcca gcccgtcacc actgtattca tgatctcgtt
    49501 gatattgtga agctgccacc gcttcggctc gtcataggtg tggcccaatg cctccttaaa
    49561 aagctgtttg gaacagacca tgctgccggt ataatgttcc agaaagccct gtatttgacc
    49621 ggcctccgtg tcctccggca tgaaatcctt ctgcacctcc acaagctgcc gctggatgga
    49681 cttgctgaat ttcatggaat acttcccgct gcggtagatg gtcatggcct ccgcccagac
    49741 ctgcaacagg taggctctgg aagcgtcctc gtcctccaaa atgtgaacct ccgctttctc
    49801 cgggtaaatc atgatgggaa ggaaacgccg gttcccggcc cggtcaaggg gcaggaagtc
    49861 cagcgtgttg gatgaaccgc cgaacacgca ctgccgcagc cggtctttgg gctgggcctc
    49921 atagggtgtg cggtaggttt ccttctggcg actgatgaac gagcggattt cctctatgct
    49981 tttggcgctg ctggtggcca gcatctctga catctcaatg atccagtggc cttgcagctt
    50041 caaaaatacc cggtcatcgt ccagcttttt caggtcatcg gagaaccact cgtcccggat
    50101 cgccagcagg cggaagaagc tggacttgcc agccccctgt ccgcccacca gacagagcat
    50161 ttcctcgtat ttggagccgg gagaaaacac ccgccggatg gctcccagca ggaagtgttt
    50221 taacatttcc tctacataat cgctgacttc tgcacctaag aagtgatgga gacaggagcg
    50281 gatgcggggc gtcccgtccc acacaaggcc gttcagcacg tcctgtatgg gatggtagca
    50341 gttctcattc gccacgatgg agagcgccgc cgtcattttc ttctcgctgg tcaggccgta
    50401 gttctgctca aaatagagaa gcagatactt cacatccgta tccgtcaggg cgctggtgtt
    50461 cctgcgccag cccaaatccc gcacgatgtc cacccggtca gtcaggaggt tcagccggat
    50521 ggcgtcccgc aggagagggt catgggagaa caccgtccgg cagttgccga tggtgttggc
    50581 gggctggccc ttctccgtga cagaaaggcc ttcccgcacc tcgtcaacgc tctgctccgg
    50641 cgctaaagct tcggctgcgc tcatgacggc ctgccgggtg gctggcggta aactctgata
    50701 ttcgctgctc aatcttcctc acctcttttc catactcagc gaccacgcag gcccgttcct
    50761 ccatactgcc ggacagcaga atatccagca gatactccgt gtacggcttt ttttgcaggg
    50821 cctccacaaa cagcgggttc catgcttcct ccggcgtctg cggggcatat tccttctccc
    50881 agcgttccag cagatgcaga taatcgcaca acacccggaa acatttctgt tccgcctgcc
    50941 ggtagcgcag ttcctcgctg atttttcgct tgcccggctt cctgcggggc gggtcatggc
    51001 ctttagcgtc aaagccgacc gagaaatcct ccgccagctt caaggccgca tctttcttgt
    51061 tcagcccgaa cagacgggcg gtaaaatcaa tcacatcccc gtccgcctga cagccgaagc
    51121 agtggaaccg gcggtcaacc ttcatgctgg gatgtttgtc atcatggaag ggacagcagg
    51181 tcatcccgtt cctgccgagc tgtatcccat aagattccgc agcctgcctt gtggggacag
    51241 cctgcttcac cgcttcaaac acattcgatt ttctcacctc cagacagaga aaaaggcatc
    51301 cggttttcac agaaaatcag atgccttaca actctatatc gttctttttg gtcggggcga
    51361 ttcgcccctc acgttcccaa cgctcccggt tatcccggtc agcattaatt tttgcctttg
    51421 ccagaatatc cataatggat tctttggctt tcttcttgac ctgctccttc ccggcttgct
    51481 tgacttccgg ctgcatcagc gtccgctgga ttttcgccag agcgtgctgc atggcgtttt
    51541 tgattttggt aatgacccca tccagacgct cagcggcata ctcacgttcc ttctttggag
    51601 ctttgcgctc cggcgaaagc acccattttt tcgattcctc caccagacga atatcttcct
    51661 tgtgcgtctc ctgccggacg gtatcggtca ccacctccac cgccttgtca taggccgcat
    51721 cggaaacatc atctaacagt gtctccacgt cctcaatttt gagcgtcagt tcttccagct
    51781 tctgctcctg cgccgccagt tgctccttct gtttcatcag gatataatcc tgtttttcca
    51841 gatagtcccg cccgccgtag gacggctcct gatccagatg tagcccatgc cgtctggcga
    51901 tgtcaaacag aatcgtccgg caaaccgcat caaaggtctg cttgcggttg ttgttccggc
    51961 ctttgggctt ctcagggttt gggagaggga tgcccagttc ttccagcgcc ttttcctgct
    52021 ggggacacag ttccccgtag cggttctcac agtcaaagac gtgccgctca tggatgtggg
    52081 gcgtcccctc gtccagatgg agcgcccagt ccaggatgtg gatatgggaa ccgaagcggc
    52141 gctcaaattc ctcataaaac tcattgacga tggaaaaaag cgtgtccggc gggacggatt
    52201 ctcccatcgt gccgatctgg tagatgcttt cctccgggca ggtcttgttg tttttcagca
    52261 agtcctccac ggtgcggttg cgctccgtgt gccgggtttt ctcattccgg gcgttctggg
    52321 cctccacatg gccgccgtaa tgctcgttgt agtacatccg ctcgatttcc tcaaagctga
    52381 aatccggctg ctccgggttc tcccggaact cgtgtgtcgt aaagccccgg tagcagtccc
    52441 agtagacatt ttgccttgcc cgttctgcgt caatgtgttc gctgttctcc acatcgaacc
    52501 ggcggtcatt gtggcgggga ttataggtgc catgcttgcc ggagcgccca ttgtgtcgtg
    52561 tcagtttcaa acagatttcc tccttcctgc gaagatgttc tggggccggg gaaggggcgg
    52621 cgaagccgca aatcctgcga attttgcgtc aggtaatacc cagtacgagt tgacgcaggc
    52681 atcaactctc cctgggcaag gcgtttcacc cttgacccga taatggctgc cgcccttaac
    52741 ctgccaatgg gcgttgcccc ttgaccccag cagtgcgctg ccgcccctgc accccggcac
    52801 aaagggctgg ctgccctctg tactcccgca ccggcgaagc tggattccac cgcagaagcg
    52861 gtgtttgcca gtttcaccgg ctccgtatcc gcacatttgc gcccataagc ggggaatgga
    52921 tacggaatac gcctgcgggg attgccggtc aaaacagcgg gtgcagaccc ggcatttttt
    52981 ccgtaaacgc cccggtttca ggcgattttt tcttccgccc ctaccacctg aaagccatgc
    53041 tcttttgcgt aggcgctggc ggcggctttc cgttcctcgc tgtacggcgg caccagccgg
    53101 atcgacagcc gggacttatc cagcacatag gtcacgctgc cctccggcgt gctgcgctcc
    53161 aaccggcaca gcagcgggta cttccggctg aaatcagcca gcctgcgctt caagctggcg
    53221 ttgaaggtgt agacgctggc aaggttttcc gcctcgttcc agttgataat ggtttctttc
    53281 tcatacttag acagcttcct catacagatc ctcctcataa tcagtgtaag atttcagcac
    53341 acgcaggctg cgcttgaggc ggcggtactc gtccatctcc atccgcagat ggtggtaaaa
    53401 atcctcgtac cagttttcgc ccacctccgt ctccaccttc cgggccagcc ccagcatccg
    53461 gcgtttggcc tccgggtcaa ccgtcagtgc cgtcagccat ttcagccgtg tcaccgtatt
    53521 gtggtggctg ggacagccgt aagcgtagag aatcttcttt tctttcatac tcagtgtcat
    53581 aatgatttcc tccgtttctt tgctgcggag ccatgcgccc cgcatgattg ataaacttgt
    53641 ttctgtctgc cggtctgcct gcaagccgct cctgcccgga atttctcccg gcgtatcggc
    53701 ctctttggtg cgctcgattt ctgtcctggc gtcacttccc cggaggccat accccttgca
    53761 gccggtcatt cgattttcaa ggttctgtgc ctgtctgcaa gaaccagttt accgaaaaaa
    53821 gaggcccttt cccgtatatc caaaacgtcg gaaaaacgct tgaaatccgc atatttccac
    53881 gcttatggaa tcgtgctata atgggtgtaa tcactgaaaa acgataacga gagagggggc
    53941 tttggatgta cgcaaagctg acaatcccgg agcggctcaa agacctgcgg gtggtagata
    54001 agcacctgac gctggaacaa ctggcggagc agaccggcct gtctaaatcc gcgctgggaa
    54061 aatacgaaac cgatgactac aaggacatca gcccgtttgc gattgcaacg ctggcggact
    54121 tttacggcgt gtccaccgac tatctgatgg ggctgacgga aaataaaaat cacccaaaca
    54181 tagaacttca atctctgcat ctgagtgatg atatggtgga acttttgagc agcggcaaaa
    54241 tcaacaaccg gcttctctgc gaactggcta cccacccgaa tttccggcgg ctgatgatag
    54301 atatggaaat ctgcatcgac cagatcgcca atatgcgggt ggagcagatg aatctggtgt
    54361 tggaggccac ccggcagacc gtcatgaaca agtatgcacc cggagaagat gacctctata
    54421 tgcggacgct ggaactgggg caggtgcagg aaagcgattt ttacagccac gtcatgcacg
    54481 atgatctgga cagcatcgtc cgggacatcc gggaagcaca ccgcaaagac aagaccaccg
    54541 ccgacccgca gcccacgctg gatgatgtga aggaaaaatt tgagcaggcc atagggcagg
    54601 gcagcgatac agaaatgctg atccacgagt tctgcgataa gttgcagata ccctttgaga
    54661 agatttcctc ggaggacttc tcggcgttcc tgcggatact gagcctgtcc aagcagctca
    54721 aaagctccaa caatatgagg ggaaaggcca gccccattcc tcccaaccgc aaggccagac
    54781 gtaagcggaa gtaaaatttc catatccgga agcggcaaaa aagccatccg ccgattgatt
    54841 ggcaaatggc tttcgtgaag ctgtattctg ttgtagtggt tgtgcccagt ttgggaacag
    54901 tcactattga agatgctatt ctttgtcttt ctgcgatacc tgtgcatggg cttcctgctg
    54961 aacaagcagc cccgcagaaa agccatattt gaagtggctt tcgttttcct cacactgggc
    55021 gattgccacc tgctcccgca gttcttcaac cagtgcatag tcctctttgc tcagacgctc
    55081 cgataaagtg tccatcaggg cggcgctggc tttgactgcc tgcttgaacc tcggtgaatc
    55141 agcgaccacc gtttcacagg gataaaatct gccgttatac atttcttcca gaatcatgca
    55201 cccacctccc tcagacaaaa atcatctcag acaggatgat ttcctctacc cggctgcgga
    55261 tgctgttcat gcccccgacc catgcaagct ggtcacgggc tttcagttcc tcggtgacgc
    55321 cctccgcagc ctgcatctgc gagatgatac acgccagtcg gtcatcggcc tgctcgttca
    55381 gatcggcaag atacgtccag agttttcccg acagaagcag ttcattgtac tggccgggac
    55441 ggtgttcttg cagaaacgcc ttgtgtatcc gaccccaccg cccgatgggg cggatttcct
    55501 ccgacaactt tagatcgggg atatagtagt ctcccaccag cgtatagctg atgccgttgt
    55561 gatggatttt ctttggtaaa tgctccatgt tcgacctcct gtattcagtt ttttcagaat
    55621 ccagttgatc gtgtccctgt tcatgtgaaa ttcaaggcaa ctgaactctt cacgaacaag
    55681 tatggcatat ccttaccagt cttttgatag tttaaaccaa agctcttttg atttgacctt
    55741 gtttccgacg tgttatacag gtcgatagtt tcttttccca gtgtttcaga caattctttt
    55801 agcgtagttt tctccttgcc accaagaaac aaggtactat cacagttacc tacgattgta
    55861 tcagcgttat ccttgtagat tgcctttagc tgagattgtg cttgaagtat gatacttgct
    55921 gaaatctctc ttgaacggat tgttgcaatt aacttctcaa acttaggaat taagccgata
    55981 ttagcgaact catcaagtag acacctcaca tggacaggta atcgtccgcc atatacatca
    56041 tcggctttat cacataaaag attaaataac tgtgagtaca taatggatac tacaaagtta
    56101 aatgtatcat cggtatcgga gataataaca aatagtgctg tttttctatc tcccagtgta
    56161 tcaagctcaa gttcatcgtc actcatcaag tccctaagtt cctgaatatc aaagggagca
    56221 agtcttgcac cacaagaaat aagaatcgat ttggcagttt ttcccgcagc caatttgtac
    56281 tttttatatt gcttcactgc aaagtgtgta ggttctttct tctccagtgc ttcaaagagc
    56341 ctatctatcg gattcatata ggtttcatta tcttctctga cttctgaagc atcaatcata
    56401 tcaagcagag ttgcaaagtt cttttcttct cttggagctt catagaagat atagccaata
    56461 agtgctgtat agtagagttt ctcagctttc acccaaaaat cttcacctga tttttctccc
    56521 tctcccttgg tgtttgcgat aattgtctgt actaatttga gtatgtcctt ttcacttcta
    56581 agataagcaa agggattgta tttcatagac tttttgaagt taatggtatt taggattttt
    56641 atctcatatc cgttatcttc aagcatctta ccgcactcaa ggactatagt tcctttagga
    56701 tcggtcacac aatatgacga gtgcatctgc ataaggttcg gttttacata aaatctcgtt
    56761 tttccacttc ctgatccacc gattacaagt acattcttat ttcttgcata ctttggattt
    56821 gccggtctgc cattcatggt taatcgttct gtttgcgtaa gtaggatatt gttttgaaac
    56881 ttttcatcca tatatggctc tatatctttt ttcgttcccc atcttgctga gccatactct
    56941 ttcccctgtc taaacttttt agcatttttc cctttggtat atacgataat tttgattaaa
    57001 acagctacta ccactcccat taaaatatcc gtaggatgaa tgcttggaag aaagctcatg
    57061 gtgttaagtt ccagtatacc ttgaaatatt ttgtctatta catcgccacc agtatatgct
    57121 cttacatgat gagaaaagat attgccaaca tagaaaaatg caagataggg aatgttctgc
    57181 tttagaaact ttgccttatc ttgcacctta aataagcctt tgatgtcttt tagtatctta
    57241 tctatcatag gctttgctcc ttttgtttat ttttgatttt ctcttttgaa atagagtttt
    57301 tagccatctc tttgaacttc tcaatattct tgtgaataga ttctttcctt tctgctttct
    57361 tttctgattt tgaaagagcg tgcttaaaag ctttatccat cactttcata tctttggctt
    57421 gaaagaatac agagttttta cctgtctctt tatttttcat cactgaaaat ttaacaccgt
    57481 atctattcaa ctctttttta agttctttaa gctcagcttc ttccactggg atttcttcaa
    57541 gctgtccctt tttaaccata tcttttagct ttacttcatt tccgttggca ttgaccagtt
    57601 tttcaagtcc tccgaatttc tccgcttctt tcatcaattt ttttaataag tttaatagaa
    57661 cttttccagt aactttacca gcatttatct ccatatttaa ggtctttctt gctatttctt
    57721 cattgatcac atcaatatcc tcctctcata tcatagctta caagagccga ataataggca
    57781 tctattccac ttggtgcatt ataagcggcg gtcaggacaa aagctcttat atttctaatc
    57841 ctatattcat tttctcttaa tactcctact aagtactcta aatgtcctga attaagtttc
    57901 ctaaatcttt cctttactac ttgtacagat atctttgttt ggttgatagt aatgaaagat
    57961 tcatcatcta ataccattac atctgccagt atctttaata tttcatcaaa ctcttcagcc
    58021 acactcttat tcccaatact catatgttca taatatcctg tttggtgtcg caattcttct
    58081 aaacaatttt gatatatatt tttatagctt atatctatcc tccctccctt catattctct
    58141 gaaaataatt gattcttttc tttgatggaa ggatggatgg aaatatatgc tgaattattt
    58201 gtagtattat ttgtagtatt atttgttgta ttctctggta atggttggtg caaattatcc
    58261 ttttgcatta gtgcattttg aacctgtgca ttagtgcaat ctgcattatt gcattggctg
    58321 attttgccct tatccattgg tgcattttgc cctaatgcat tgggcataga attttttaat
    58381 gcttctgttt gtattttttg ttttttcaaa tccatatcca tatataattc ttcaagttta
    58441 tcattattaa ttgtatacca ttttgtttta tctcttggat ccttattgta atttccaact
    58501 aaaagataac ctgctttttc taactttgaa aaagttcttt ttactgtatc aaagctcata
    58561 tagtcaaagt cattttcttg ccatgctcga attgaattat atgtccagta tttaccatca
    58621 tgataattct ttcctgattt tttatttatt tctatccaat aattaatctg ctgtagtatg
    58681 agtgcttcgt ttaatcctat ttctctagca agagttttat tcgcaactat aggttgttca
    58741 tcaaataaat acataactca tcaaacactc ctttcttttt ttcaaacaaa aagacgatag
    58801 ttatttctat cgtctaaaat catatataaa tatcattttc taaatagagc gtattgcttc
    58861 tttcactccc gttaaaacgg ctataacagc tgttccaacc attggtattc cgtatgggct
    58921 taacaaaaat cctaatatca atgcttctat ccctatggta acctcttttt gtaaaaaaga
    58981 tgctattgct ccaaatatgc aaaacatcat caacaggtaa agtattgctg ttcctatgcc
    59041 aagtaaaaat gaaagaaatg caatgagaat acttaacgct atactaattg gaaataagat
    59101 aatttttaat atccatctca tatatactct ccatttatac taaatcattt taaaatgtct
    59161 taaagcgtcc attatagctt cttctttttc aggcgtacac tgtgggatag cctgattgtc
    59221 ttttttagat ttattataat gctctctcag ttcaattcca catttcttct taatctgtgc
    59281 aatataaagt gtagaaacct ttagcccaaa tttttctaat acatactcct taatttgagc
    59341 atatgttgct ttgctctctg cacttgtcaa atcaagctca tccagcttaa tttcaacatc
    59401 tatatgctta tcgacatcaa gtttggacaa taacgctacc gtctccacat gtgtagaatg
    59461 agggaacata tctacaggtt gtgcctcaac acatttaaat ccgtgaacat ttagatagtc
    59521 taaatccctt gctaaagtag aaggattaca agaaacatag acaattcttt ccggattcat
    59581 tgaaacaata gtctctaata ccttttcgtc acatcccttt ctaggtgggt ctacaactac
    59641 tacattggca gtcttaccct ctctatacat cttaggtaca atttcctctg cctttccaac
    59701 ataaaattca gcattatgta tattgtttaa tttagcattt atcttagcat cttttatggc
    59761 atcatctata atttctattc cataaacctt tttagccttt tgtgccaaaa ataaagatat
    59821 tgtacctatc ccacagtaaa tatcaaaaac agtatcagta tcatttaaat ttgcatattc
    59881 aagagcctta tcatacaata ctttagtttg gacagggttt acttggaaaa atgataatgg
    59941 tgaaatctca aacactaagt cacctatata atcgtttatt tttccatctc catatataac
    60001 tatattttct cttcctagaa tagcattcgt atctttttga tttacattta caaccaaagt
    60061 cttaaatcca ggtatatttt ccttaagcac tgaagctaat tctttaagat atggaagttt
    60121 gtttccattt gctactaata caaccattac ttctttagtt gtaaaaccta tttttgttac
    60181 taaatgccta atcaaccccg tgtgagtctt ttcatcatat atacttacct tataggccct
    60241 tatatatgtt tttattattt taacaatttt atcatttatc tcatgttgta ttacgcattt
    60301 ttctgtcgat attacatcat gagtcttctt tttatagaag ccaataattg gaataccatt
    60361 attcttttgt attggaaatt gtgctttatt tctatatcta tatggattat ccattcctat
    60421 agtgtcatga acttctacat cctctaactt acctattctc gaaagtactt gtttaacttc
    60481 atttgttttc atttcaagtt gtttattata atctaaatct tgtatctgac accctccaca
    60541 atccctaaga ttgtcactac acactctttt caccctgaat ggagactttt ctattatctc
    60601 aacaatatct ccctcagcat aatttttctt tactttatta atcctaactt taactttatc
    60661 atgtattaat ccgccctcta caaaaactgt aataccattg tactttccta ctcctactcc
    60721 gccttgacca atgtctacta tatctacaat atacatctcg tttttcgaaa gcataaatca
    60781 ccctccaatc aatctattat atttaaatct ttacaaactt ttttctttaa atttatatta
    60841 aaatattgct attaattaaa ctatattttt tactaagcta caatatttta ttttaccaaa
    60901 aaaaaaggag cttataaagc cccttagtaa ataaatttat tatttttctt ataaaactct
    60961 agttgaacct ctatatacca cgcctcttga tgcatccaca gttacaactt ctccatcttt
    61021 tactagattt gttatatcag ttgcagacac aataactggt atatctaaat ttatacctac
    61081 aatagctgta tgtgatgtca ttccaccatt ctcagttact acagctcctg ctttttctat
    61141 ataattgttc atttcagcat cagtagcagt agaaacaagt atgtcgcctt cattaaagtc
    61201 tacacaagca tttccattct ttattatacg aactttacct tctaccgttt gagtacctac
    61261 acctatacct tgcacgattt cttcgcttat tacatgaacc tttattaaat tagtagttcc
    61321 acttactcct actggtactc cagcagttat aactactaat tccccattat ctatataatc
    61381 agcttgtcta gctgcctcaa ttgccttttc tataacttca tctgtatttc ctgcaacaga
    61441 agacttaact ggatatactc cccatgtaag agctaattta ttcatagtct tttcattatt
    61501 tgtagtagct attattggag attttggtct aaatttagat accattctag cagtatatcc
    61561 tgaagaagta gatgttatta tagctgatgc atttaaatct actgctgttg tacaagttgc
    61621 atggcttatt gcatctgtta cagtaacatt gttagtacca ttttccttta gtaatctatc
    61681 ataatctaaa gtctcttctg ttctttttgc tattgtagcc atcattttta ctgcctctac
    61741 aggatatttt ccagcagcag tttctccaga taacattatc gcatctgttc catcatatat
    61801 agcattggca acgtctgtaa cttctgctct tgttggtctt ggatttctta tcatagaatc
    61861 aagcatttga gtggcagtaa caactggctt tgctaattca ttacattttt ttatcatcat
    61921 tttttgaact attggcatct cttcagttgg tatctcaaca cctaagtcac ctctagccac
    61981 cattatacca tcagaaactt ttaatatttc atctaggttc tctacacctt cttggttttc
    62041 tattttagat attatttgta tatcagttgc attattgttc tctaatactt ctcttattgc
    62101 tagtacatca gatgcctttc ttacaaaaga agcagctata taatctattc cttgacttat
    62161 tccaaactct atatcactta tatctttagg agttattgct ggtaaattta tcttaacacc
    62221 aggtacattt actcctttat gatttttaac tattcctgaa ttttcaacaa cacaaactat
    62281 atcttctcca tttatttctt ttactcttaa gccaactaaa ccatcatcta ttagtataga
    62341 atctcctgat ttaacgtctt cagcaagacc tttatagctt actgtacaca tttctttagt
    62401 ccccataata tctttcatag ttatagtgaa cttttgccct tcttctaaaa atacctctgg
    62461 gtcttcaaag ttacctgttc taatttctgg acctttagta tctaataata tagcaactgg
    62521 cttatttaat ttttctctaa ctttctttgc catatcaatt ctttctttat gttcttcatg
    62581 cgaaccgtga gaaaagttaa atctacacac atttagacca ttttgcataa gttgagttaa
    62641 aatttcctca ctttgtgatg ctggacctag tgtacaaaca atttttgtct tctttacatt
    62701 atttaacata tttgctcctc ctgagatatt attccacaga tttattttta atactaaaac
    62761 ttatatagat aatatttttg ccatctcata agcttcttta tcaaatactt tcttttgagc
    62821 taaagcttca cttatttcta aatcaattat tttgttttct tttattccta caactctagc
    62881 tgattttcca tctaataata attctactgc tcttactcca agtctacttg ctagtattct
    62941 atcagataca gttgggcttc ctcctctttg gacatgcccc aatacagtga ctcttaaatc
    63001 tgctcctgac tcttttttaa gttccttttc taaatcacta gcacttccga ctccttctgc
    63061 taaaacgatt atactatgtc tcttacctct tttttgagta gtctttaatc ttagagcaac
    63121 ttcatctact gtaatttcta cttctggtac tattatagtt tccgctcctc ctgcaagacc
    63181 tgcatataaa gctaaatctc cacagtgtct tcccataact tctactatgt taactctttc
    63241 atgagatgat gatgtatctc ttatcttacc tatagcatct ataatagtat tcattgcagt
    63301 atcaaatcct atagtataat ctgtatatgc taaatcgtta tctatagttc ctggtatgcc
    63361 tatagctggg aatcctaagt cactaagttt ttgggctcct gcaaatgaac catctccacc
    63421 aattacaact aagcaatcaa ttttatactt tttaagaatt tttactgctt ttaatctacc
    63481 ttcttctgtt ttaaattctt cacatctgga agattttaat atagttcctc ccctgtgtat
    63541 aatatctcca acagaagata agttcatttc agttaaatct tcttctagta atcccttata
    63601 tcctctattg attccatata ctttgcatcc ataatatatt gctgatctta caaccgctct
    63661 aatcgcagca ttcattcctg gagcatctcc tccacttgtt agtaatccta tagttttcat
    63721 tttttacctc cttggatttt atgtcaattt tttattataa tcattgttat tgtattctaa
    63781 tggacttttt ttgtccctat atcacttttt aatcccatca agactaaaaa aattaaatgt
    63841 ccttaaatgt ccattttgtg atattatatc acaaaacgga caatattttt catattatta
    63901 taatatattt agatttttat ataaaaaatc aagcttttac ttttatgtta tctttaggaa
    63961 gtaactcttc taagttattt ataaattcat cacatataca agtcaatgcc ccggtcattt
    64021 tatatgcttt acgtttacca tttatatttt catccatagg gtacaagtat atactgtcat
    64081 tacctggata ttgtttggct atttctacaa tattattagt tgtatcagta tcagacatac
    64141 tatctatttt caaatacaat tttttaccac tttctggctt aatattttct acatttctag
    64201 ttcttcctga attgtagttt ctatctgcaa agtcagtatc atcatttata tcttttatct
    64261 ctctagctat aagttttgca ttttcgcctt cttttatact taatgttcct tttacatata
    64321 ttatattatc ttcattcaaa atgacattat acttttgtaa taattgagga aaaactatta
    64381 tttctatagc accatacaaa tcttctaatt caataaatgc cataatttca tttcgttttg
    64441 ttgttttaat agttttagtt acaatcattc cacccattat aacatctgtt tcattcattt
    64501 gtaggtatga ctcttcttcc tcttttaatg aatttaattt tccattatcc atagatgttt
    64561 tttcttgtag ttctttttca aattgtgata aaggatgtcc acttacatac attcccaaga
    64621 cttctttttc aaggttaagt ctctctcttt cttcatactc ttgcctttta ggtaaactgt
    64681 atatttgttg aaaatcacta tcttcctcac tatctccaaa agcatcaaat aaagaaattt
    64741 gaccttgaac atttttcttt ctatccatag atacactttc taataaactt tcataaccag
    64801 ccatcagact agctctattt tcacttatat tatcaaaagc tccacatttt attagacttt
    64861 ctattactct cttatttgta tctttttggt ctaatctttt gactaaatct actaaatcta
    64921 caaataatcc attagattct ctttcagcga ttatattgtt tattatattt acaccaacat
    64981 tttttacagc tgctagacca aatcttatat tgtcgccttc aactgaaaac tttgaaaaac
    65041 ttttgtttat atctggaggt aatacatcta tttctagtgc tttacattct cttatatact
    65101 caactacctt atcagtattt cccattacac tggttatcaa tgctgccata aattcaactg
    65161 gataatatgt ttttagataa gctgtctcat atgctaaaac cccatatgca gctgcatgtg
    65221 atttattaaa tgcatatcta gcgaagtcta tcatatcatc aaatatttta ttagctattt
    65281 cctcttctac accatttctg atacaccctg caatttctat attaccatct tcatcaaatt
    65341 taccatgaat aaaatattgt ctttcttctt ccatcacgtc catctttttc ttactcatgg
    65401 ctcttctaac taggtcactt cgtccgtaac tataaccacc taaatctcta acgacctgca
    65461 taacctgctc ttgatatacc aaacaaccat atgtaacttc cattataggc tttaattttt
    65521 catgtagata tgttacatct tttgggttgc ttttattttt tatatatgca ggtatagaat
    65581 ccattggacc tggtctaaat agggatattc ccgctacaat atcttcaaaa ttgtctggtt
    65641 ttagctgttt cataaaactt ctcatgccag cactctccaa ttgaaacacc cctagtgtat
    65701 ttcctgatga aagcatttca tatactttcg ggtcgtcata atccatcttt gaaaagtcta
    65761 ttttttctgt gtagtttttt ataactctat ttttttctat taaatcaaga gtatctctta
    65821 taactgtaag agttcttagc ccaagaaaat ccatctttaa aagcccaagt tcttcaagtg
    65881 ttgtcatagt aaactgtgtt gtgattgcat cttgatgttt gtacaaaggt acatattcat
    65941 ctacgggatt ttttgatata acaactcctg ctgcatgagt agaagcatgt cttagcattc
    66001 cctctatctg ttttgatata tttattattt cttttgtttc cgggtcttgg tcatacaatt
    66061 ctttaagatt aggatttgta tctagggcct tatctattgt cattcctaaa gcaaatggta
    66121 tttccttggc taccttgtca actttattgt agcctatatc caataccctt ccaacatctc
    66181 ttattgcagc ttttgcaccc attgttccaa aagttattat ctgggctaca tggtcatcac
    66241 catattttct ctttacataa tctataactt cttctcttct ctcataacaa aaatctatat
    66301 ctatatctgg catggatata cgttctggat ttaaaaaacg ctcaaataat agagagtact
    66361 ttattgggtc tatatcagtt atcttaagtg tatatgctac tatcgaacca gccgcactac
    66421 ctctacctgg tcctactata atattatttt ctttagaaaa atttataaaa tcccaaacaa
    66481 ttaaaaaata ctctacataa cccatttttt caattacatc taattcataa tttaatctct
    66541 ctagtatctc attgctagga ttatcatatc tctcttttaa tccattaaaa cacaactccc
    66601 ttaaataagt ttctggtgta tatccttctg gaacatcata cttaggaaga tgtatagtat
    66661 taaaatcaaa ctccacatta cacatatcag ctattttcac agtattgtca atagcctcta
    66721 atgcgtatgg aaataattct tccatttctt ctcttgattt taagtaaaac tcatcactac
    66781 caaatctcat tctatttggg tcatttaatg tttttcccat ttgaatacac ataagaacat
    66841 catgtatttt cgaatcttct ttatttacat agtgaacatc attagtagca actagtggta
    66901 taccagtctc ttttgataat tttactaaac cagagtttac ttctttttgc tcaggtaaat
    66961 tgtgatcttg aatctccaaa aagaaatttt cttctccaaa tatgtctctg tagtcaagag
    67021 caaattgttt tgctttttca taatttctat tcattaaagc acgtgctaca tctcctgcaa
    67081 ggcatgcaga aagtgctata atccctttgt tatgtttttt aagttcttcc atgtcaactc
    67141 taggcttata gtaaaaacct tctacgtatg aagttgaaac tatttttatt aaatttttat
    67201 atccttccat attcttagct aacagaacta aatgtccttg atatttgtca tagtttgggt
    67261 ctttatctct caatccacgt gcagctgtat agacttcgca ccctataatt ggctttattc
    67321 cttcttttat agctgttttg tagaaatcta taacaccaaa catacaacca tggtcagtta
    67381 tagctattga gctcatgttt agttccttag ccctttttat aagttttttc accctcgaaa
    67441 acccatcaag taaactatac tcagtatgta catgaagatg agcaaaatct ttgttcatta
    67501 aatcacctcc tgctttataa ttttaatttt accacaaata tatgtcctaa aacaaaaaca
    67561 gagactttta tctcaaaata ggaattaact tcaacatata ccattatatg ttataaatta
    67621 atgcttattc tgagattgtc ccctcaattt attgctatga tttttttttc aaatctgttg
    67681 caatttcttc tatttttcta agtctatgat tgactccaga ttttcctatt ggtggttcta
    67741 acatctctcc aagttctttg agagtcatat cctcatactc gattctaatc ttagctatct
    67801 cttgtaaatt ttctggcaaa ctgctaattc ctatagtctc ttgtatgagc ttaatatttt
    67861 ctacttgtct tactgctgca tttactgttt tagaaaggtt agctgtctca caatttacta
    67921 tcctgtttac attatttctc atttctttta ctattttagt attttgaaga cttaaaagag
    67981 cgtggtgtcc tcctataata ctcaacaaat ctgatatttg ttcactctct ttgatataaa
    68041 caacgtgatt attttttcta gccacaattt tactattaaa acctaaagag tttataagtt
    68101 cttttagaga atctgcaaac tcctcattat tagttacaaa ttccatatga tagttctttt
    68161 ctgggtcgct tatagaacct ccaccaagaa aagctcctct tatgaaagct ctcttacatc
    68221 cttcatgctt aattaagttc tctgaaattt tgtttatagt aaaaaagcct tctttatctt
    68281 caagtatacc taactttttt aataacattt ctgaacccat ttcgcttgtt accattaata
    68341 cataactgtt atttctcttt agcatttgat tcttatttac tgagattgta gtattgatat
    68401 taaaattctt ttttaataat ttaaatactt tacgggctat tgaatttaac tctgttgtta
    68461 tctttagatt tagcttctta tatccaacga tttgtatact tccccctgat ttaactagag
    68521 ctgataattc agctatatta caacattcat tcccagatat aactcttgct agttcatttt
    68581 tggtttcagt tgaaaaagac atatctatca cctattaact aatttttctt taaccttttt
    68641 tttatttctt gtaaggcaac ctttattatc tccttttcat catcatacct agctaggtct
    68701 accactctat ctagatggat aaatttagca tcaataaagc cttcctcttt ttgtggaata
    68761 gtatccatat tctttgcttg catcaaatac caaaaaactg ttttatgaac tgctctattt
    68821 tcatcccagt tttctttgaa tgtatagtgt atttcaccta aatatttcaa tatatctgct
    68881 tttactccag tttcttcact tacttcacgt aatgctgcct cttggttatt ttccccttct
    68941 tctacttttc ccttgggtaa cacccaatct ccattgaact tccttagtaa aagaatcgta
    69001 ttgccaaaca ggactacacc accagcacta acctcttctc tcatcgatgt tcactcctta
    69061 aaactaacat aatgataagg atttctacac aattttttaa aatccttttt tactaacttt
    69121 tgttgtaatt atgatttagg gctaactcaa taactatgtt agatatatac tttgcatcat
    69181 gtcttatata attatttttt atttcaatta aattcttctc tacggttttt atacccatct
    69241 cttttaattt atctttttgc tttttatcta acaagacttg ctctgctcca tcttgtttat
    69301 ataagtcaag catttcttct ggcaatatct cattatttgc gattacataa tctattaaat
    69361 tctcatcagt gtgctttact atagcattaa catgctctaa tacattgtac ccttctgtct
    69421 cacctggttg agtcattata tttgctatat atacttttgg agctacagac gatttaatag
    69481 cgtctacaat cccctctaca agtaaattgg gtattatact cgtatataag cttccagggc
    69541 ccataattat aatgtcagca tcgtaaatag atgcaataac atcctttaat ggtttgacat
    69601 cttttggatt taaaaaaatt ttatcaatag agcttttttg aatttttgac tcttcaggta
    69661 tactagattc tccatttatt atattgccat tttctaattt ggcaactaaa tttacatctt
    69721 ccaaagttac tggtaaaact cttcctgtta tagcaaaaat ttcacttaat ttatatacag
    69781 ctttttcaaa gtttccatac aacccattca tagcagccaa aaacaaattt ccaaaactct
    69841 gaccttttaa aagcccttct gtaaacctat attgcataac ttcattcatt gttggctcta
    69901 tatttgcaag tgctaataga cagtttctta tgtctcctgg tgggagcatt cctaagtctt
    69961 ctctcaatac acctgaacct ccaccatcat cagcaacagt tacaattgca gttatatttt
    70021 gcgtagtgtg tttaagacct cttaaaaata ctgattgacc agtacctcca cctatcacaa
    70081 ccacatttgg ttctctattt tcaacaatag acctttgaaa tagttttact ttttcatttt
    70141 ttgctttttt atatataaat aacgatacaa ttaatgcaat ccaacctaaa attcctacaa
    70201 taataagtaa tttagccatg tttgcctcca ttatcgcaac atagagtctc tatgtttctt
    70261 tacaactcta tatcccttat tagataaatc ctccgctata agatttgtta tagtaactga
    70321 cctatgtctt ccacctgtgc atccaacacc aattactaga tgttgttttc cttcatctat
    70381 atattgaggt actaaaaaat gaatcatatc taaaagtttc acataaaatt cttcactgat
    70441 tttagaattc ataacataat ctctaacttc tttatcatca ccagttttag ctcttaactc
    70501 ttcaacataa tagggatttg gaagaaatct tacatcaaat actaaatctg catcagcaag
    70561 tataccatgt ttaaatccaa aagatactac agaaatagtt aggtttggat tatcttcacc
    70621 agaagaatat attttcttta tttcttcctt aaggtcttta ggtttcatgt tagttgtatc
    70681 aattatacat gtagataact cctttaaagg ttccattatc tttctttcca tttttatacc
    70741 ttctggaatc tgcatatctt ttgccaatgg atgatttctt cttgtcattt tatatctctt
    70801 taacaataca tcatcattac agtccaaata taccatttca tattcataat tttctttttc
    70861 taaataattt aagctttcat gaagcgcttc aaaaaacttt ctccctctta tatctatccc
    70921 tagagcaact ttatcaatac ttgaatttgg ttgataacat aattctgcaa atttagttat
    70981 caaagcagga ggtagattat ctacacaata aaatcccata tcctctaaag ctctcattgt
    71041 ctcacttttt ccagatcctg aaagacctgt aactataaca aatttcattg ctaactcctc
    71101 ccttttatct tatgtttatt atattataat gttttctact aacgatatat ccagattaga
    71161 gtcagatata gtcttcatag ctagtttaaa atcaccaact ctttctggac aatgtgcatc
    71221 tgagcctatt ataaatttat tttctagccc ttttatttta tttaattgat ttacattcaa
    71281 aaaagaatgg ctactattta tttcaagtct agtatttgtc ttttttgctg tctttgctac
    71341 ttcttctata tacacatcgc ctttatctcc tggatgagta attataaaaa tatcattatt
    71401 acttatcgca tttattacag cttttgtatt gtattctttt gctttttcag ttttaaaaat
    71461 atagttatta caatgattta ataagcttct taatgatgtt ggagtagaac caaaatgata
    71521 tccagccata atataatcaa aatctctaat aaatttatca tccatatcta tattgccttt
    71581 atcatctaat atgttacact ccatacctaa aagtatattt atatcattgt acttatcatt
    71641 taaataatct acttcttctc tcattttgag aatatctttt ttctttacac catatgcaat
    71701 atgtttatag ctatggtcag atattcctat tgtttttatt cctttagaaa tggctacctt
    71761 tacattatct tcaatagtcc ctttgccatg actatataat gtatgtgtat gataatcagc
    71821 cagtattttc atttgttaat cctctccaag tattttgact tcttcttcca acataacccc
    71881 aaacttggca tatacagcac tttttataac atatattaaa tccaaaatat ctttagcact
    71941 tgcttctcct tgattgacta caaatccaca atgcttctca gatacttgtg ctcctcttaa
    72001 agtaagacct ctcaatccag tttcttctat taatttacct gcaaaatatc ctgttggtct
    72061 cttaaatgta cttcctgcac tcgcaaaatt taatggctgt ttagttattc ttcttgtcgc
    72121 taaatcatcc attaaacttt ttatttcatc ataatttcct tcttgtaatt ctagttctgc
    72181 tgataaagct atatatccgt ttttcgatat tatacttttt ctatacccaa attccatctg
    72241 ttcatttgat aattcaaata tattgccttc catatccatc agtcttactg acttcactat
    72301 gtccttcatt tcaccaccat atgctccagc attcatggct agtgctcctc ccaatgtacc
    72361 aggtattcct gatgcaaact caaacccttt taattcttgc ttttggagag ctttacctaa
    72421 aactgataat aaagctcctg actgagcagt catcctagta tcatttatct cataagaatt
    72481 gaaattatca gctaattcta ttaccactcc tcttattcca ccatctttaa ttaaaatgtt
    72541 tgaaccattt ccaattatta aatacggaat agattcttct tttaccaact taagtacatt
    72601 ttttaattgt tcttcagttc taggtctaac taatatatct gctggtcctc ctaccctaaa
    72661 tgaaatatgt tttttcatag gttcatcgac ttttatatcc tctttactta atatatttaa
    72721 taaatttata tatatatctt gattattcat tgtatttcct cccaaattat actgaacttt
    72781 agctgtcatt attatttaaa ttacattagt atatagtatt aactatattc aataatatat
    72841 gattttgctt atcatatgga ttttatattt taagcttctt aattacaaac tatattcaat
    72901 aaatatataa taaagtaaaa aaaaaaaaat aacaacatat aaaagaatat aaataattta
    72961 aaaaaagcat caatatcact gatatcaatg ctttaaaagg caatttaatt cgtattattt
    73021 atctaactat ttttgacata gcaaagataa taaatccctc caactactat ggaacctcca
    73081 acgatatttc caagcgttac aaataccaaa ttagttataa attgaaccat agttatattt
    73141 gctcctaatg ccattcccat tggtataaaa aacatgtttg caacactatg ttggaatccg
    73201 cataatacaa ataacattat tactgtccaa catgaagcca attttgacat tatatcttga
    73261 gatgctgtag ccatccatac agctaaaacg actaaaatat tacaaagtat tgcttttaaa
    73321 aacattgatg ctatagttga attaacctta gcttctgcca cagctatagc ttttgtagct
    73381 gcatctcctg tcaatgtaga agaataaaac acaagtgcaa ctagcagaat tgaacctact
    73441 aaatttgcaa aataaaccag tccccagttt cttaacattt ttcctaatgt tatttttttg
    73501 tccataagtg caatagccat taaggtgttt ccagtgaata attctgctcc tgccaccact
    73561 acaagcataa gacctactgg aaagaccata gcacttaaaa agctagctac ccctgggtct
    73621 atcttgccaa aagtttgact aactatcata ctccccaatg ccccaaaacc tataaatgct
    73681 cctccaaata aacctaatag aattgcattt tgtgtcgaaa tgtttgcttt tttaatgcct
    73741 gcacttatag ttgcttgtgc agtttcccca ggtgtcaaga atcctttact cataaatcct
    73801 tcccccttaa aaattatact ttcccttgtc tttttatggc aataattata taataacaat
    73861 atgatatata ataaatttta ttaacataaa aaatatacta tatacttata tat
//
LOCUS       DAMJWP010000003        67573 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.003, whole genome shotgun sequence.
ACCESSION   DAMJWP010000003 DAMJWP010000000
VERSION     DAMJWP010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 67573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67573
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.003"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(121..894)
                     /locus_tag="PC700_000155"
     CDS             complement(121..894)
                     /locus_tag="PC700_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HDF2579487.1"
                     /translation="MKNVANATHYLNMDTKELFNLYNKNKNVDIRNILIERHLYLARL
                     LAKKYINKGVDFEDIYQVASLALIYAIDRYDVEKGFEFSSFATPTIVGEIKKYFRDKV
                     WTLRVPRRIQELSKKISDAKIKLEQENKKHPKVKDIADYIGVSEEDVLEAMEASYGYQ
                     PMSLDSSSNDDSEDKDITLIDKIGKEEGNFGSIEYEDFINKFIETLNELEVKIFKDRF
                     FFDKTQSSIAKELGISQMTVSRLEKKVVEKLKKEYEKNL"
     gene            complement(894..1280)
                     /locus_tag="PC700_000156"
     CDS             complement(894..1280)
                     /locus_tag="PC700_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2579488.1"
                     /translation="MEITTNPDYVSIIRLTASGIANKMGFPIDDIEDIKVAVSEACTN
                     AIKHSKDDVFNIVFNILDNAINIEIQDNGKGYDVSSISTPDLENPKESGLGLFIIKTL
                     MDDVDIESEHNQGTKIKMTKYLGVDI"
     gene            complement(1305..1622)
                     /locus_tag="PC700_000157"
     CDS             complement(1305..1622)
                     /locus_tag="PC700_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HDF2579489.1"
                     /translation="MSMNIDSNLDSQNKFWNVCLDGELDVSTADKLKEHLHALIEKNM
                     LDVKINLKDLDYIDSTGLGAMIGVLKKLKINEKEIYIVNPKSNVKKIFTITGLDKIFK
                     VEG"
     gene            complement(1634..1894)
                     /locus_tag="PC700_000158"
     CDS             complement(1634..1894)
                     /locus_tag="PC700_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HDF2579490.1"
                     /translation="MNAWGWQIGAVLVGSSALIVAIYLAKTLNSINKVVEKAYKIVDY
                     NERHIQDIVENASSISKGIDDIVYVINKILSIGNIFKIIKRK"
     gene            complement(1894..2190)
                     /locus_tag="PC700_000159"
     CDS             complement(1894..2190)
                     /locus_tag="PC700_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDF2579491.1"
                     /translation="MCNKRSCKKGGFLLLGAILGFIFGMFFAPKKGSELRKETKEKFN
                     DVKENPKEVLHETFNDVKERIINLVDDDNNEEDIKISEEDIVISKSFDDEGDVN"
     gene            complement(2259..4685)
                     /gene="gyrA"
                     /locus_tag="PC700_000160"
     CDS             complement(2259..4685)
                     /gene="gyrA"
                     /locus_tag="PC700_000160"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HDF2579492.1"
                     /translation="MEENNKILPIEIAEEMKKSYIDYSMSVIAGRALPDVRDGLKPVH
                     RRILYSMSELNLTPDKPYRKSARIVGDVLGKYHPHGDIAVYYAMVRMAQDFSTRALLV
                     DGHGNFGSVDGDSPAAMRYTEAKMSKLSLELLRDIEKETVDFKPNFDESLKEPSVLPA
                     RYPNLLVNGSNGIAVGMATSIPPHNLAEVIDATVYLIDNPECSVDDLIKFVQGPDFPT
                     AAIIMGKESIAEAYRTGRGKVKVRSRAFIEELPKGKQQIIVTEIPYQVNKAKLVERIA
                     ELVKEKRIEGISDLRDESNRNGMRIVIELKRDANANIVLNNLYKHSQMEDTFSIIMLA
                     LVDGQPRVLNLKQILYHYIKHQEDVVTRRTKFELNKAEARAHILEGLKIALDNIDAVI
                     SLIRASKTGQEAKIGLIEKFKLTEIQAQAILDMRLQRLTGLERDKIEAEYEDLIKKIN
                     RLKEILADERLLLNVIKNEITIIKENYSDERRTEIRHAEGEIDMRDLISDEEIAITLT
                     HFGYIKRLPSDTYKSQKRGGRGISALTTREEDFVRHLVTTTTHSRLLFFTNKGRVFKL
                     NAYEIPEGKRQAKGTAIVNLLQLSADEKIATLIPIDGNDENEYLLLATKKGIVKKTKR
                     EEFKNINKSGLIAIGLRDDDELIGVELTDGKQEVLLVTKEGMSIRFDENDIRYMGRTA
                     MGVKGITLSKEDFVVSMNLCSKGTDVLVVSKNGFGKRTNIEEYRSQIRAGKGIKTYNI
                     SEKTGTIVGADMVNEDDEIMIINSDGVLIRIRVNEISLFGRVTSGVKLMKTNDEVNVV
                     SIAKINIEEE"
     gene            complement(4705..6606)
                     /gene="gyrB"
                     /locus_tag="PC700_000161"
     CDS             complement(4705..6606)
                     /gene="gyrB"
                     /locus_tag="PC700_000161"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003226808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDF2579493.1"
                     /translation="MKQEYGASQIQVLEGLEAVRKRPGMYIGSTSPRGLHHLVYEVVD
                     NSIDEALQGYCSDIYVSINEDGSVLVKDNGRGIPVEIHPKTGKSTLETVLTNLHAGGK
                     FGGGGYKVSGGLHGVGVSVVNALSKWMVAEVYLNGKIYKQTYEKGLPTSKLEVVGESQ
                     DKGTMIQFMPDETIFDEIEFKYETLEYRLRELSFLNKGIKIVFEDKREGQEKRKEFHY
                     TGGLVEYIKYLNKSRTGIHDDIVYIDKKVDDCFVELAMQYTDGYTENIYSFANNINTH
                     EGGSHLSGFKAALTKTVNDYAKRNKFLKENDVNLLGEDIREGLTAVVSVKLPEPQFEG
                     QTKTKLGNSFMRGIVDSVTVDELGSFLEENPSTARIIVDKALRAQRAREAAKKARELT
                     RRKSVLESTSLPGKLADCAEKDPSKSEIFLVEGDSAGGSAKQGRDRNSQAILPLRGKI
                     LNVEKSRLDRILSSDEIKNMITAYGCGIGEDFDIDKARYHKIIIMTDADVDGAHIRTL
                     LLTFFFRYMRPLIDEGYVYAAQPPLYKVTKQKKEHYVYSDKELNILLDEIGRNGVELQ
                     RYKGLGEMNAEQLWETTMNPETRTLLQVTVEDAAIADEVFSMLMGDKVAPRKEFIEEN
                     ARFVRNLDI"
     gene            complement(6607..7722)
                     /gene="recF"
                     /locus_tag="PC700_000162"
     CDS             complement(6607..7722)
                     /gene="recF"
                     /locus_tag="PC700_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDF2579494.1"
                     /translation="MKLKSLQLVNFRNYKKLHLEFNGKVNLLVGKNGQGKTNIVESIY
                     MLSFGKSFRTNKDKEMVRFNSENLYIGGSFSKYNKYSLIELIIGKDKKGIRINKVPLQ
                     KIQELLGNLNVVIFSPEDLRLVKEGPKERRAFIDKEISQIIPKYYKYLTNYNKTLSQR
                     SRVLKNIHVDEALLDVYDDTLAKYGSYIYILRRDFIKKIANISENMHMNLTNGVERLS
                     IRYKNQINITDEDTIDTVYNKFLAKLSSNRPNDIESKTTRYGIHKDDLNIFINDLDAR
                     LFGSQGQQRTASISLKLSEIELIKNEVEEYPVLILDDVFSELDEARQKLLVNNLSNVQ
                     MFITSAEVSHKKIFDEKNVTIFNIENGDVISIENGGN"
     gene            complement(7740..7946)
                     /gene="yaaA"
                     /locus_tag="PC700_000163"
     CDS             complement(7740..7946)
                     /gene="yaaA"
                     /locus_tag="PC700_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HDF2579495.1"
                     /translation="MTEITIESEYIKLDQFLKLAEIASTGGHAKFLIQEGLVTVNDEI
                     ELRRGKKIKSGDIVKIEGTKIKVL"
     gene            complement(8083..9189)
                     /locus_tag="PC700_000164"
     CDS             complement(8083..9189)
                     /locus_tag="PC700_000164"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDF2579496.1"
                     /translation="MKIICNQKILANRIGIAQKAINGKTTIELLKGILISTEEGQLKL
                     TGYDAEIGIETYVQAEIIEKGDVVVDARLFGDIIRKLPDSFVEIETDSENNIYINCVN
                     SRFKIKGYAAKEFPKLPELNEEDLYSIPQEILKNMIKQTVFAISQDQTKPVLMGELLE
                     IVDRNLNLVAIDGYRLAVKSCSVDSLTENIKVIIPGKTLIDVNSLLSGEDNVKVGFNE
                     KNAIFIINDTKIITRLLEGDFIDYKKLLPREHNSRVKLNTKELLNSIERASLLSQSEK
                     NNLIKLSIRDKVMAITSNTEKGNVYEEVEIDLDGDYLDIAFNSRYFIEGLKNIDNEEI
                     FIEFTTNVNPCIIKPTDDVNYIYLLLPVRISSNI"
     gene            complement(9431..10753)
                     /gene="dnaA"
                     /locus_tag="PC700_000165"
     CDS             complement(9431..10753)
                     /gene="dnaA"
                     /locus_tag="PC700_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDF2579497.1"
                     /translation="MMDIVSLWDKTLQLIKGDLTSVSFNTFFKNIVPLKIHLNDLILL
                     APSDFNKDILENRYLHLIEDAISQLSLKKYNIKFVLSEKEVADLNSDSTDLNYRVLYP
                     NLNPKYTFDTFVIGNSNRFAHAACVAVAESPAKAYNPLFLYGGVGLGKTHLMHAIGHH
                     IVSQKKDSKVVYVSSEKFTNELINSIKDDKNEEFRNKYRNVDVLLIDDIQFIAGKERT
                     QEEFFHTFNTLHEANKQIIISSDRPPKDIPTLEDRLRSRFEMGLITDIQAPDFETRIA
                     ILRKKAQLERIDVPNEVMSYIAKNIKSNIRELEGALTRVVAYSSLSNRVISFDLATEA
                     LKDIITTSKNEEINVLRIKEKVSSVFNLKMEDFNSKKRTRSIAYPRQIAMYLTRELTD
                     LSLPKIGEEFGGRDHTTVIHAHDKVSKDIEESEEIKTKIDKIISDLKG"
     gene            11409..11546
                     /gene="rpmH"
                     /locus_tag="PC700_000166"
     CDS             11409..11546
                     /gene="rpmH"
                     /locus_tag="PC700_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDF2579498.1"
                     /translation="MSKRTYQPKKRQRSKEHGFRKRMKTSNGRNVLKRRRAKGRNRLT
                     H"
     gene            11624..11968
                     /gene="rnpA"
                     /locus_tag="PC700_000167"
     CDS             11624..11968
                     /gene="rnpA"
                     /locus_tag="PC700_000167"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDF2579499.1"
                     /translation="MDFNRTKGLKKDSDFRKVYKHGKSFANKYLVIYILKNKSDYSRV
                     GISVSKKVGKAITRNRVRRLIKEAYRLNIDEKIKPGYDIVFIARVSSKDATFKDIDKS
                     IKNLVKRTDISI"
     gene            12000..12251
                     /gene="yidD"
                     /locus_tag="PC700_000168"
     CDS             12000..12251
                     /gene="yidD"
                     /locus_tag="PC700_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDF2579500.1"
                     /translation="MNKIIYILKEISRYLSNLCIYLVRFYQKYISPLKGPTCRFYPTC
                     SQYSIEAFKKYGFVKGMYLTIRRLLKCHPFHPGGYDPLK"
     gene            12283..12990
                     /locus_tag="PC700_000169"
     CDS             12283..12990
                     /locus_tag="PC700_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDF2579501.1"
                     /translation="MNIIGNFLGLILKVIFEFVNHYGISIILFTILVKIILLPLTIKQ
                     TKSTKAMQDIQPRIKEIQEKYKNKPEKQNEEIVKLYGEAKINPLSGCLPLLIQFPILI
                     GLFSVLREPVAHGVFANKAAFLAADNGFLWIKSLTSPDYVLAVFSGASAYVMQKVMTP
                     KDQLQGSMKVMTYVMAGMSFYWGFIFPAGLTLYWTVSNLFSIAQYYLIMNPLKAKLAA
                     NSKEEKVNEKKPKINKK"
     gene            12959..13588
                     /locus_tag="PC700_000170"
     CDS             12959..13588
                     /locus_tag="PC700_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HDF2579502.1"
                     /translation="MRKSLKLIKSKSKEEAISKAIAELNLKAEDIEVEILENPSKGFL
                     GFIGAKDGTYEIFVIEREVDVAKNFIEVMLKNANVDAKVNVSQKDNLIMVDIEGKEAA
                     SLIGRRGETLDSIQFLTGLALNKINKDSHTRVLVDIENYRSKREESLIRYANKVAREV
                     AKTRKTKKLDYMNPYERRIIHSALQNDKYVITYSEGTDPYRRLVIECKR"
     gene            13700..15079
                     /gene="mnmE"
                     /locus_tag="PC700_000171"
     CDS             13700..15079
                     /gene="mnmE"
                     /locus_tag="PC700_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDF2579503.1"
                     /translation="MFIDDTIAAIATAPGEGGIGILRISGERALKVAEEIFKSMSGKS
                     IEEYNKRTLIYGNILDNENIIDEVLLAYMKGPNSYTGEDVIEINCHGGFISVKKILEL
                     ILSKDVRLAEAGEFTKRAFLNGRIDLSQAEAVIDVIKAKTDIAHEVAQNQLEGSLSKK
                     IRELRDKVTEILAHVEVAIDYPEEDIEHITYQTLKEKTDELKKEIKKLYDTAESGKIL
                     REGLKTVIVGKPNVGKSSLLNSILGENRAIVTDIPGTTRDVIEEFVNIKGIPLKIVDT
                     AGIRDTDDIVEKIGVEKSKESFTSADLIVMVLDASRKLSEEDIEILEKLKDKQTIVLL
                     NKNDLKQEIEEEKILKYVENNSIIKISALQQEGIEELQDKIESMVYKGSIKNNSSLVV
                     TNSRHKDALSKAYKSATDALIALEQSMPFDFVEVDLKNIWDYLGYINGDTVTEDLLDN
                     IFHNFCIGK"
     gene            15174..17069
                     /gene="mnmG"
                     /locus_tag="PC700_000172"
     CDS             15174..17069
                     /gene="mnmG"
                     /locus_tag="PC700_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDF2579504.1"
                     /translation="MIKFEAGKYDVIVVGAGHAGCEAALATARMGYKTLIITMSLDSI
                     ALMPCNPSIGGTGKGQLVKEIDALGGQMGLNIDKTYIQSRMLNTAKGPAVHSLRAQAD
                     KFKYHEEMKKTLEDEPNLDIAMDEVVEILHEGNVVIGVGTKLGCSFKSKAVILATGVY
                     LNSKIYMGEVAFYEGPNALGYAKYLTDSLVELGLRMRRFKTGTPARVHRDSIDFSVMS
                     LQEGDEKVTPFSFMNENIEKKQEPCYLTRTTEETQKVILDNLKRSAMYSGVIESTGPR
                     YCPSIEDKVVRFSDKTSHQLFIEPEGLNTKEMYIQGISTSLPFEVQLDMYKTIKGLEN
                     CKIMRPAYAIEYDCVDPTQLKISLEIKGVENLFSAGQFNGTSGYEEAAAQGLMAGINA
                     VRKIEGKEPFVLDRSEAYIGVLLDDLVTKGTNEPYRMMTSRAEYRLYLRQDNADMRLT
                     QKGYDIGLVKKDRYERFLNKKAAVDKEFERLKNERVTPKEVNSLLEEKGATPIKVGIS
                     LYEFLKRPEVTYELLEELGKGAGEDVSREVKEQCVIITKYEGYIEKQLKQIDQFKKLE
                     NKKLDEKINYSSIEGLRLEARQKLDDIKPISIGQASRISGVSPADISVLLIYLEQVRR
                     TRGGKGE"
     gene            17062..17781
                     /gene="rsmG"
                     /locus_tag="PC700_000173"
     CDS             17062..17781
                     /gene="rsmG"
                     /locus_tag="PC700_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDF2579505.1"
                     /translation="MNNIELLKNGIEGFNIDTNDSMLEKFKIYREILVEWNKKMNLTG
                     IEDEKEVYIKHFLDSISAVKNGYIKNGMSIIDVGTGAGFPGIPLKICLDSLELTLLDS
                     LNKRINFLEEVSRVLELDNITFIHGRAEDFGKNEDYREKYDVATARAVAGLPILMEFC
                     VPFVKVGGYFICLKGPNANLELEESQKAIDVLGLEYIEKIDVELPEVDLNHNILVFKK
                     VMETPVKYPRKAGRPAKNPIK"
     gene            17956..18741
                     /gene="noc"
                     /locus_tag="PC700_000174"
     CDS             17956..18741
                     /gene="noc"
                     /locus_tag="PC700_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HDF2579506.1"
                     /translation="MEDKRVMEIPIEDVVPNPYQPRKIFSQVSLEELSNSIKVYGIIQ
                     PITVRAKDGKYELIAGERRLRAAKLAELKTIPAIINNMNDESSAVLALLENLQREDLN
                     FIEEAIGYENLIKEHAFTQQQLAEKLGKNQSTVANKLRILKLPNDIKMKLIENNLTER
                     HARAFLKLPSEDLMQSVLDKVIKNELTVKKTEKLIQDVLEETKVQEEPDKKQNIKGAM
                     SIRIYINTIKQAFDAISNTGIDAKYNEIDKGDYMEVVVKIPKK"
     gene            18876..19649
                     /locus_tag="PC700_000175"
     CDS             18876..19649
                     /locus_tag="PC700_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDF2579507.1"
                     /translation="MGKVIAVFNQKGGVGKTTTNVNLSASLGTLGKKILVLDLDPQGN
                     TTSGYGINKNEVENTIYEIMLDGLHIKEAIISTEFENVDVVPSATELSGAEIELTSKT
                     NREYILKNSIKAVIDEYDYIFLDCPPSLGMLTINCLTAVDSVLIPIQCEYYALEGVSQ
                     LMETIKLVKSRLNADIEIQGVVLSMFDGRANLSIQVVEEVKKYFKGSVYTTLIPRNVR
                     LAEAPSHGKPVIYYDKRCRGSVAYLELAEEFIDLEEEEW"
     gene            19651..20514
                     /locus_tag="PC700_000176"
     CDS             19651..20514
                     /locus_tag="PC700_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDF2579508.1"
                     /translation="MENKSKRSNRLGRGLSALIPEIKGETSEKEIVNIDIDKIYPNEV
                     QPRKQFDEEKIKVLSDSIKNYGVLQPIVVKMDENNKYMIIAGERRFRASKLANKNQIP
                     AIIKDIDMKDIMEIALIENLQREDLNSIEEALAYKSLIEHYNVTQEEISEAVGKSRPH
                     ITNTLRLLNLGQDVIEMIDSGRITAGHGKALLRIADKDLQLQIAKKIEEEELSVREVE
                     NIAKKISENKQEEPKKSKPKDVFILDVEDKLRNIFGTKVNISKGKKKGKIEIEYYNDD
                     DLNSIVSMLLE"
     gene            20547..21689
                     /locus_tag="PC700_000177"
     CDS             20547..21689
                     /locus_tag="PC700_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDF2579509.1"
                     /translation="MIYLDNAATTYPKPERVYNAVLDCMKNYCANPGRAGHKLAMRAA
                     REIYDTRENIAKLFNVSNPMNIVFTSNATDSLNLAIKGVLQEGDHVITTSMEHNSVIR
                     PIKALEKRGIENTVVKCDYEGFLDYEDLEKSIKSNTKLIVTTHASNVCGTLIDIKKVG
                     EIAKKHNILFLVDASQTAGVYDIDVNECNIDMLAMPGHKCLFGPQGTGILYVREGLNL
                     NILKEGGTGSKSEEIVQPELFPDKYESGTHNTPGIAGLNQGILFIFERGINNIRQHEE
                     ELCQYMIDKLEEVSDIKIYGPKDSKKRASVIALNIGDMDSGEVTFLLDSDYNIATRSG
                     IHCSPLAHTTLGTLKQGAVRFSIGYFNTKDEIDKAVEALKKISKNK"
     gene            complement(21823..22398)
                     /locus_tag="PC700_000178"
     CDS             complement(21823..22398)
                     /locus_tag="PC700_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HDF2579510.1"
                     /translation="MKNKKIMLVLSILSISIFAVGCTNAQNGSDTSKKETKSNTNVEQ
                     PKEENNTEKEVPNNKAKPTPKEETKTQSATIYSFDVDKTDLIENKVDLNKIDENTLFE
                     ELQKLKVVPESAKLNSFTTKDIDGVKTGILDVSSDFTKSNLGSDAETLMLDSVARTYI
                     KNMNVEQIKITVDGSNYESGHIVLEEGDYLK"
     gene            22658..23950
                     /locus_tag="PC700_000179"
     CDS             22658..23950
                     /locus_tag="PC700_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF2579511.1"
                     /translation="MEILSLGEKIKKLRKEKNMTLKELAGDRITAAQISHIERDKSHT
                     SYELLEYLGDKLDVSVEYLLETKEMQSKKITDNLILQSEIYIKENELNKAEDQIKEVL
                     DICKRYDLMENYGRCNFLMANINLKKSNYNDAIENFEKALYFFIKNSDSENILKCYLN
                     IGKIYMQEDFYKGAISHFNFAEEILSENKFEDVNIHKELYSKMAYCYIKLDNPNMSLK
                     YIEKINEIDNRNDRKEDVDILVLKANNMLEIGKYEESRDYFKKALKILESEDNKTGLA
                     NVYLTICDVYRKIGETDRVLEYSQKVYDIKKNDEDEYMMSGLFKIIEAYIDKEDYDLA
                     RKYCKIALASSIKNKNKFNEYKALRFYANMYKNQNEVTLAIEYLIKCIKIVSDLGNNR
                     ILANLYIDLGQLYSNISKEKELEYYQKGVFMYKNLEIM"
     gene            24055..24657
                     /gene="yedF"
                     /locus_tag="PC700_000180"
     CDS             24055..24657
                     /gene="yedF"
                     /locus_tag="PC700_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HDF2579512.1"
                     /translation="MSIKIDARGLACPKPVINTKRELDNLEEGVVVTIVDNETAKENI
                     LKLAKSLSCEANIVDEKENFISIEIRKGKNILDKQNVVDNKESELDNTCIFISSDKMG
                     LGNDELGKVLIKGFIYTLTESKPYPKYVLLVNGGVKLSAENEETIENLKILENMGVEV
                     LSCGTCLDYYNLKDKLQVGTVTNMYTIVETLKNASNTISI"
     gene            24730..24993
                     /locus_tag="PC700_000181"
     CDS             24730..24993
                     /locus_tag="PC700_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HDF2579513.1"
                     /translation="MNQMYIVSFNSTHHAIRSEKLFGENSLKVMALPTPREITASCGI
                     SIKFSFEDMEKIKTILVENNVDIKGIYCISKLENGSKEVEKLD"
     gene            25008..25193
                     /locus_tag="PC700_000182"
     CDS             25008..25193
                     /locus_tag="PC700_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HDF2579514.1"
                     /translation="MPMDLKIGDIVELKKQHACGCKEFEIIRTGMDIKIKCTKCSRLI
                     MLDRETLEKRVKKLIKK"
     gene            25296..26492
                     /locus_tag="PC700_000183"
     CDS             25296..26492
                     /locus_tag="PC700_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2579515.1"
                     /translation="MAVKYAKRMQGLQGSEIRELLKLTQQPQIISFAGGMPAPELFPV
                     EEMKKVSVAVLEENGRSAMQYTTTEGYEPLREKIAARMNDKNKTNVNKDDILVTSGSQ
                     QGLDFAGKVFIDEGDVILCESPSYIGAINAFKSYQPKFIDVPTDSDGMIMEELEKILE
                     TTDRIKMIYVIPDFQNPTGRTWPLERRKKFMEIVNKFEIPVIEDNPYGDLRFEGETLP
                     SLKSMDTKGLVIFLGTFSKIFCPGYRLGWTCASPEILSKFNFAKQGADLQASTISQME
                     VSKFMDMYDLDAHVDKIKAVYVKRRDVMLKTMEEEFPEGLVFTHPEGGLFTWVELPSN
                     LNAKELMPKCLEKNVAYVAGGGFFPNGGRENTFRLNYSNMPEEKIIEGIKNIAAVLKE
                     AMGVEA"
     gene            26706..26984
                     /locus_tag="PC700_000184"
     CDS             26706..26984
                     /locus_tag="PC700_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDF2579516.1"
                     /translation="MRNYELVYVVKPNSDEEVREAILNKVKEVVATDGEIVKVDTWGT
                     KKLAYPIAKFTEGFYVLVNFKSAVDVPKEIDRNLKINENVIRHMIVVA"
     gene            27021..27455
                     /gene="ssb"
                     /locus_tag="PC700_000185"
     CDS             27021..27455
                     /gene="ssb"
                     /locus_tag="PC700_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF2579517.1"
                     /translation="MNQVVLVGRLTRDPELRYIPGTGTPVASFTIAIDRDYVKKDGSK
                     ETDFIPVEVMGKSAEFCANYITKGRLVALQGSIRVDNYQTQSGEKRTFTKVSTRSVQA
                     LDSKNKSENSYRESAPAFEPSFEPQGLDPQGFQAIDDDDIPF"
     gene            27474..27701
                     /locus_tag="PC700_000186"
     CDS             27474..27701
                     /locus_tag="PC700_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDF2579518.1"
                     /translation="MMNKKRRKKKRVCQFCADKNAKIDYKSTQRLQKYITERGKILPR
                     RISGTCAKHQRELTVAIKRARNIALLPYTLD"
     gene            27844..28164
                     /locus_tag="PC700_000187"
     CDS             27844..28164
                     /locus_tag="PC700_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDF2579519.1"
                     /translation="MKIDKGSEITRNIKIIEWMKTEILMSVSDLFNLLFKGVKPLDEA
                     IQDTLANIIMITYLLAKRLGISFRDVDYKVKEKIKIGIDEDHSVERWYGDLSNLKKHM
                     DNRE"
     gene            28172..29128
                     /locus_tag="PC700_000188"
     CDS             28172..29128
                     /locus_tag="PC700_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HDF2579520.1"
                     /translation="MNNKIRLSKAMSIIVLAIIIALVIAYIPMLGIFSMLAAVPYVII
                     GAITDRKYSLISILVTFCVLILFADISYALNICIMYSLPGIVIGKMLKRSFEQEDSNK
                     FEPIYGGTIIFVLSMLVYFFVLKTFLNVNLLDEVSKMISEIVNIQKNSFSATELKVFD
                     KMKPDEIVSYFTNMLPMMLFLQGLLSAFVTYYLSVFFIKRITKMNIRFPKFADFYLPG
                     NAILTTFLLYLLVLFIQIIGSKLYTELIMTNLQLVFNLMFVIQGIAVCIYFLKKWIRQ
                     GPNKIMFFSGIILCLFGFMGISFVGMVDSIIDFRKVRSYKST"
     gene            29139..31133
                     /locus_tag="PC700_000189"
     CDS             29139..31133
                     /locus_tag="PC700_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HDF2579521.1"
                     /translation="MSNKQTFKLNMPEINLYIIVIGISSIILLYYNLYVGCLFFCIFV
                     YMVFHNWRTTNIRRHEWTEYIQNLSLDIDETTKKAIINLPIPLCILEFDGNISWYNGK
                     FYDMIGQKDLLDKNIEDIVKNLNLRKVLNENKEMYTEINYKEKEYTIIYNVIKNDQEK
                     NPKYLMILYWIDKTEYLKVKQNYDDEKNAMMLIQVDGYDEVLKSAAEDKRALINVEVE
                     KILSALELNSNGALRRTSKDKFFLVMHKKELKKLEAEKFSILDTIRHIDYGNNLPVTI
                     SIGIGIDGDTLNENLKLATGALDLALGRGGDQAVVKTKDKFVFYGGKSKAVEKKTKVK
                     SRLIGHALREVIQQSDQVYIMGHKYPDMDAMGAAVGVYDICKSCNKTANIVLQSVNES
                     IEIFINKINENNYYKKLFIGKEEAIDNCTKNTLVVVVDTHRPNYTECEELLKLSEKVV
                     VIDHHRRGVEFINDAVLLFHEIYVSSTCEMVTELVQYMDEDVTINKLTAEGLLAGISL
                     DTKNFAFKTGVRTFEAASYLRKVGADTIEVKKFFNSDVKDFIIKAEIIQSTKIINNRI
                     CLAYSSTEIDSINVIIAQTADELLNIKEVEASFVLGEKDDTIFISARSLGQINVHVLM
                     EKLGGGGHIDIAGAQLKNVSLKEAYKMVNKIIEEYLEEEE"
     gene            31134..31583
                     /locus_tag="PC700_000190"
     CDS             31134..31583
                     /locus_tag="PC700_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDF2579522.1"
                     /translation="MKVILLKDVKGTGKKGEMKEVSDGYARNFLFPKKMAVQADSVAI
                     KELNEKNKSKEIKAQKEYEEAVLLGKQMEEINIEIYSKAGEGGRLFGSITSKEIAEQL
                     KKQKDIDVDKRKILLDEPIRSLGSTFVEIKIHQKVTTKIRVDVKEKQ"
     gene            31602..32930
                     /gene="dnaB"
                     /locus_tag="PC700_000191"
     CDS             31602..32930
                     /gene="dnaB"
                     /locus_tag="PC700_000191"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDF2579523.1"
                     /translation="MEDMTRIPPHSVESEQSILGSILLDKDAIITVTETIKPDDFYKE
                     AHKIIYECMITLSNKGEPIDLITLTEELRKQGHLNDIGGISYITSLSTIVPTTSNVKY
                     YADIVKEKSVLRKLIKASNEIINLGYSGATKIEDVLEQAEKSIFDISQEKTSDDFKSI
                     NLVLMDAYDMIEKLYTNKSDVTGITTGFKDLNKKINGLQRTDLILIAARPAMGKTAFS
                     LNLVQNAALKGDASVAVFSLEMSKEQLVQRMLSSQSSVELKKIKTGTLNDNDWPRIID
                     AMAVLSDAKIHIDDTPGIKISELRSKCRKLKIEKGLDLVLIDYLQLMEGEGNNESRQQ
                     EISKISRSLKILAKELNCPVVALSQLSRAPEQRADHRPMLSDLRESGAIEQDADIVMF
                     LYRDEYYHADSESKNIGEVIIAKNRHGETGSVELVWLGEVQRFGDKLRDL"
     gene            33135..33443
                     /locus_tag="PC700_000192"
     CDS             33135..33443
                     /locus_tag="PC700_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2579524.1"
                     /translation="MEKISLKYIYPNIIKVLDEINLFRVIDNNLRESIVVYANNVDNQ
                     YHINMTNTNFGNIINICKLEKLLDVDKFMEKVIKYEKEIIEKEEFSKIEEYMLNIGEY
                     "
     gene            33525..34814
                     /locus_tag="PC700_000193"
     CDS             33525..34814
                     /locus_tag="PC700_000193"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDF2579525.1"
                     /translation="MKTVAIVGSQWGDEGKGKVIDYLATQADVVVRGQGGNNAGHTLV
                     VEGKKYALHLIPSGVLNPNTVNIIGNGIVFDPKGFLEELEMFKTDNISTENIKISDRA
                     HVIFPYHKELDALSEEARGDLKIGTTKKGIGPCYMDKTERSGIRICDLMDKDKFAIKL
                     KAQIDAKNKIVKNIYGKEELFDFETIYNEYLGYAEQIRKYVADTSVIVYDAVRAGKKV
                     LFEGAQGTLLDLDLGTYPFVTSSHPTSGGFAIGAGIGPNMIKDVVGIVKAYTTRVGEG
                     PFVTEQINETGDKIREQGHEFGVTTGRPRRCGWFDAVIVKYAARVNGLTSISFMLLDV
                     LTGFDKIKVCTSYKMGDKIITDFPASLDDLAKCEPVYEELDGWNEDITQIDNFDDLPE
                     NAKKYVAKIEELVGVSVDMVSVGPNRAQTIIRRNIFA"
     gene            35140..35215
                     /locus_tag="PC700_000194"
     tRNA            35140..35215
                     /locus_tag="PC700_000194"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35173..35175,aa:Lys,seq:ttt)
     gene            35222..35296
                     /locus_tag="PC700_000195"
     tRNA            35222..35296
                     /locus_tag="PC700_000195"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35255..35257,aa:Glu,seq:ttc)
     gene            35301..35376
                     /locus_tag="PC700_000196"
     tRNA            35301..35376
                     /locus_tag="PC700_000196"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35334..35336,aa:Val,seq:tac)
     gene            35410..35484
                     /locus_tag="PC700_000197"
     tRNA            35410..35484
                     /locus_tag="PC700_000197"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35442..35444,aa:Thr,seq:tgt)
     gene            complement(35867..36859)
                     /locus_tag="PC700_000198"
     CDS             complement(35867..36859)
                     /locus_tag="PC700_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2579526.1"
                     /translation="MNEDKIRKILAKYAKRRDDNELLLEHRKNEVYNRIPEIKSIDDE
                     ISKIGLSLAKIVLLNPKSKDEIVKKTKENIESLKVKKEKLLSESNIPLDYLEIKFQCI
                     SCKDKGFLPNGEKCSCLKQEIVNEAYKMSNLDRILSQENFSNFNLNIFSPKKGSDGEI
                     SPRENMLNNLSICENFVHDFKKDNSENLLFYGSTGLGKTYMCNCIAKELLDKGNVVIY
                     QTSFRILDILEDYKFRRDTNNQISEDNYKNLFDCDLLIIDDLGTELNNSFTSGEIFNI
                     VNTRLVAGKKIIISTNLTPSQIGNTYTQRTLSRILDKFRILEFTGDDLRWERFK"
     gene            complement(36868..37926)
                     /locus_tag="PC700_000199"
     CDS             complement(36868..37926)
                     /locus_tag="PC700_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDF2579527.1"
                     /translation="MFFKESNEIDLGEITIPNIFIDIFMPMADGLYVKVYLLGYRQAC
                     DITSNPKFDNNSIAKNLNIPLSDVLSAWKFWEEKKIIKIHDNGEYDNFNYSIEFLDLK
                     NFYIENILSNNSSIKSNTDKVVSTSENPSIRKMFNSINKIVGRYLDPSEKMSIMDIMN
                     KYNMSPDMILCAYEYVKDKTGTSKPVKYIEGIIRNWYDSNLYTPKDVEESFLVRSERY
                     ILYKTIFNELGFSRQPSKSEKELMDTWFDKFNMDIDLIINACSKSKNISNPSISYING
                     IIKNWNEKNIKNLNDLKQKEEERIVKENINKKQINTTQNNNTYKKTKFHNFNETFTQY
                     TSDELDEIIKKSQKEKFK"
     gene            38051..39514
                     /locus_tag="PC700_000200"
     CDS             38051..39514
                     /locus_tag="PC700_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HDF2579528.1"
                     /translation="MVIKFNQKVRRTLSVAILFMVLIIGCFIEKDDSLETYKEKTNSK
                     INQYNMDVIFDDETKRLMCNQNVDYINNTKSNIDKIYFHIYPNAFSKKDFAPFEKSEM
                     TRAYPNGFNEGYIDIKNILNNNSKMEYKIKGDKNDILEIELGKELKPNERMSLDIKYN
                     VKIPNSIGRFGYGENTINVTNWFPIACVNDERGWNLKSYETIGDPFYSETSDFHVKLL
                     IPNKYKIAHTGKLIDDKHDNKKMLYEIEAENVRDFAFILSSKFDVNKVDSKGVAINTY
                     NLNKKLSKKATEVAKDSINIFSELFGKYPYKEFSVVASDFFIGGMEYPMLVMIDQSLY
                     NEKNEFLLEYVIAHETAHQWWYSAVGNDEISEPWLDEALTEYSTIVYFEQKYGKDMAN
                     KLIKTMEIQTKSYLSENIFKPANEYKNSTEYSLNVYTKGAIAFNEIRKEVGDKVFFET
                     LNEYYNKYKHKNANGRAFVELWNSKGVDINKIISECK"
     gene            39616..40413
                     /locus_tag="PC700_000201"
     CDS             39616..40413
                     /locus_tag="PC700_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2579529.1"
                     /translation="MLKYCSVGSGSSGNCHYIGYKNTNILVDAGLSGKRITTGLKDIG
                     VDADKLKGIFITHEHVDHIKGAGILSRKFDIPVFANIKTWCSMKDKLGDIKDKNMKVF
                     ENDKTYSLGDIIVRPFSIEHDASDPVGYNFYTESDEKMSIATDIGCITQNIKKHLYKS
                     KLVVLESNYDPNMLMMGSYTYALKKRVMSSTGHLSNEDAANFCVELINEGTESILLAH
                     LSKENNFPELAYETSKGVLASNDIVVGQDVKLDVLSRNDVSNVYEMK"
     gene            40426..42528
                     /locus_tag="PC700_000202"
     CDS             40426..42528
                     /locus_tag="PC700_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC/EAL
                     domain-containing protein"
                     /protein_id="HDF2579530.1"
                     /translation="MLKRNFENVEDNKILYSIKQGMILAIPAIMTGSTALVILNLPIK
                     AYQEYLSGLFNGEVTNILNFINDSTLGIISLIILLTISYSYGKIYGSKYTVLVPIVAM
                     CSFLVFSHGNELNSYIEIFKTKWLFTSIIVSMTSSVLFVKLTESFVTSVKFHTEGADA
                     DFNMVVSAIVPFIIVVFLFSIFRVIMISLIGSSNFQDIFYNLFSNVFNKMGTNLMSAL
                     LFIFLMHFMWFFGIHGSNVLDTVAKNLFENSMAININLVNNNQLPTEIFTKTFFDTMV
                     LLGGCGSLLCLVIAIFLSEKRINVRKLAKIASIPALFNINEMLVFGIPIVFNYIMFVP
                     FVITPIILTITSYVAMSTGIVPCTVSAVEWTSPIFLSGYMSTGSIRGSLLQVFNLCVG
                     VMIYIPFIKMSQNRYTYMFKNNIENLTNLLKRYELTGEQPNLLNHNGKIGSISKMLAS
                     DLKFAMKRGEIELFYQPQVNYNGNVVGAEGLLRWKHSIGGFIYPPLVIILAKEEGFLD
                     ELGEYIINKACIDLKKSEKLLRNPVKFSVNISPDQLDDPKLPEQIKNIISSNDINPNM
                     LGIEITEQIALSGSQIIIERINAIHNLGIKLIMDDFGMGHSSLIYLQNNNFDIVKLDG
                     SLVKEILTNKRSSEIIMTIVNLSKNLDFDIVVEYVENIDQRDKLYGLGCEIYQGYYYS
                     KAIPFEEFVEYANQEKEI"
     gene            42596..43075
                     /gene="rlmH"
                     /locus_tag="PC700_000203"
     CDS             42596..43075
                     /gene="rlmH"
                     /locus_tag="PC700_000203"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDF2579531.1"
                     /translation="MNISIVVVGKIKEKYLKLGIDEFKKRLSKYCKLEIIELDDEKAP
                     ENLSVKEMEIIKDKEGKKILGKIKHNSYVIVLAIDGKGLSSEELAKTMSDLAVRGNSS
                     LCFIIGGSLGLSDEVLGRADYRLSFSKMTFPHQMMRLILLEQIYRAYRINNGEPYHK"
     gene            43224..43535
                     /locus_tag="PC700_000204"
     CDS             43224..43535
                     /locus_tag="PC700_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2579532.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMESAAKENGIESQIWAIPESTIQN
                     EIEKCDVLLLGPQVRYVLPKAQEIAKPYNIPVEVINMMHYGTVNGEAVLNRAIELNNT
                     K"
     gene            43583..43900
                     /locus_tag="PC700_000205"
     CDS             43583..43900
                     /locus_tag="PC700_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2579533.1"
                     /translation="MDEKIIEISFNIIGYAGEGKGLAFEAIKEAKNGNIEKAKELLKE
                     SKEVINKAHRYQTELIQNEASGNRTEISVILIHAQDHLMNGMNFQQLAEEIVDLHLKL
                     QDK"
     gene            44113..44814
                     /locus_tag="PC700_000206"
     CDS             44113..44814
                     /locus_tag="PC700_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2579534.1"
                     /translation="MLRNQPLYIQVVDILKQKILNNEYKIDENIPTERELEQEFGVSK
                     ITIRKAIEILESEGYVEKKSGKGTKVISNSLFNKLSKAESFSSILLSKGHKLSKKTIS
                     IEKIINDTNSQVFSIFGEKCSKITRMYYLDSKPYIFFTHYIPFDENLTVESFNNESSL
                     YMYLYRRNINITRFEDEFFIEESELEVSKALNLAKDILLGRKRKTYDENDNIVELSFA
                     RYNTEVQNYVIKYEI"
     gene            45038..46339
                     /locus_tag="PC700_000207"
     CDS             45038..46339
                     /locus_tag="PC700_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2579535.1"
                     /translation="MEKFASFLERKIMPVAAKISNQRHMRAVRAGIIATLPLTIVGSF
                     FTILLNIPIDAYMDFIAPFKATLDIPFRYTVGFLSLYATFGIASSLAKSYDLDTTGVG
                     MLAVMAFLVSTIVPTQVVEPVGGVIEAGRWMDMAKLNSPSLFGAIVTSLITVEIYRFM
                     KEKNITIKMPAGVPVEVSNSFTALLPTAVILMLFWVIRHILGFDISVALSAMLSPLKG
                     FLSGNSLGGGLLTVFLITLFWVLGIHGPAIMGPVIRPIWDMSIAENITAFTEGVSAHA
                     MPNIFTEQFLQWYVWIGGAGATLALVVMFMFSKSQYLKSLGRLGFLPGLFNINEPIIF
                     GAPIVMNPILGIPFIIGPIIMTILSYVLTITGVIPMMVARLPFAMPAPIAAVMSTNWS
                     ILAGILVLINFVISFAVYYPFFKVFEKQQLQREQEEMSENL"
     gene            46476..47570
                     /locus_tag="PC700_000208"
     CDS             46476..47570
                     /locus_tag="PC700_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDF2579536.1"
                     /translation="MERRLGISVYPEHSNMEDDKAYIKMVSKYGFKRIFMCMLSITRP
                     KEEVKKHFKEIITYARELGFEVILDIAPNIFDILQISYDDLTFFSELNASGIRLDYGY
                     DGSKEAMISFNPYDLKVELNMSNDVAYLDNIITYKPNVNNIYGCHNFYPQRGTGLPYD
                     FFVRCSNRFKKYGIRTAAFVNSKYATIGPWNINDGLCTLEMHRNLDIATATKHLFATG
                     LIDDVIVGNAYASEDELKRMSEVNEYQVVFNVVAKEKTSEVEKIIMVNEQHFRRGDIT
                     NQVARSTEVRKKYAKENFPKHDNKNIFKRGDVVIGNDDFGKYKGELQLILQDCTDERK
                     NKVGRIKEDEIILLDFIEPWSKFKIILEGE"
     gene            47572..48705
                     /locus_tag="PC700_000209"
     CDS             47572..48705
                     /locus_tag="PC700_000209"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HDF2579537.1"
                     /translation="MKIDILIKCGKTVDKSIIDIAILDDKIIEVKNHIDDSKYQAKQI
                     INLDGEKFISAGWIDIHTHCYERLDLYKDYPDEVGIKSGVTTVVDAGTTGALDIGEFY
                     EDTKKYKTNVYALINIAKQGITSQDELSNMMNIDEYELKNAVRKYKDFVVGIKARMSK
                     SVVISNDVEPLKVAKRIKNELNLPMMVHFGSSPPTIEDIFDYMEKGDILTHIYNGKPN
                     GILRGNEVKKEIIEAKERGIILDVGHGTESFSMDIAMKSKDAGIFPDTISTDIYIKNR
                     INGPVYNLSTTMEKFIYMGYSLEDIIDKVTKNAADAISLKNKGLIKEGYDADLTIFDV
                     VNEEKELQDSLNKSVITSTSIKPRAVVVNGEYLNIGNSIEENE"
     gene            48723..49832
                     /locus_tag="PC700_000210"
     CDS             48723..49832
                     /locus_tag="PC700_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HDF2579538.1"
                     /translation="MSSIFEKYSVRRVINASGKMTILGVSTVDKEVIEAIGEGCKNFL
                     IIEELVNKTGEYIANLLGCESALIVSSASAGIAQSVGAMIAKDDMSLVYNVNNPKKQV
                     KREIIIPKGHNVDYGVPVEVMIRLGGGEVVEAGYANMCNKEHIIREINENTAAIMYIK
                     SHHCVQKSMLSVLDAVQVAKEYNLPLIVDAAAEEDLNVYYELGADLVIYSGAKAIEGP
                     SSGLVIGKKKYIDNVKLQSKGIGRAMKIGKENIVGLTCAIERYISNDKVTLKEMEDKL
                     NPFIDKINTIKGVSASITRDSAGREILRGEINFNEDIINKSTQQIINELRSGEIAIYT
                     RDYKANEGKIEIDIRSVTGSDLDNIYNRIKNIVES"
     gene            49897..50637
                     /locus_tag="PC700_000211"
     CDS             49897..50637
                     /locus_tag="PC700_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HDF2579539.1"
                     /translation="MNCIPQYYKNRVCLNVLAGSIKNAKEVYDACDGHVLVGVLSKNY
                     STVEAAIEDMKKYSKEIDNAISVGLGAGDPNQSNMVSQISKELQPQHINQVFTGVATS
                     RALLGQNDSIINGLVSPTGKPGFVKISTGPLSSKEKDGIVPIETAIAMMKDMGGSSIK
                     FFPMGGLKTKDEYIEVAKACAKHNFYLEPTGGIDLDNFKEIVQIALDAGVEKVIPHVY
                     TSIIDKETGDTKVEDIGVLYKIMKELLG"
     gene            50642..51634
                     /locus_tag="PC700_000212"
     CDS             50642..51634
                     /locus_tag="PC700_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDF2579540.1"
                     /translation="MNILAFGEIMMRLSVPDYKFLTQTNELNYIITGTGLNILSGLKN
                     FGYNTYMLTKLPNNNVGKASSANIRKLGVKDDFITYGGNHIGVYFLENGYGERPSEVT
                     YLNRLNSSFCESKLQDYDIDKCLEEMDIVHICGIAMQLTDSVKKIALELARKSYEKGI
                     KVFFDFNFRQSLNPERTYDDLVNDYKKILPYCHGLFASYRDINKILNIDSLNIKSSSE
                     LIKKEYDIDIVAGTMRRFDDMNNRFIKGYISNDDGYFESREYKLEIYDRVGAGDGYVA
                     GIIYSYLENMKSEDIVEFGVASSVLAHTTYGDNSLVSTGHVLRLMNNEKIDILR"
     gene            complement(51821..52702)
                     /locus_tag="PC700_000213"
     CDS             complement(51821..52702)
                     /locus_tag="PC700_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2579541.1"
                     /translation="MNTKQLEYFISVAENLSFTKTAEKFYISQTAVTQQIKALEEQIN
                     VTLFTRSKRHVELTPAGKVFLSEARTIIKNINDAIAKTQQFAHGFFGTLSIGTLIGYE
                     KNKLQQYLKEFSNTYPNISMDICTNEITELLNLVKNNSMDLAFVINPENQPLKDFEYK
                     TVERYSLVALLPPGHPLYNEDSIDLIELQHDKFIFVKETGDEYGQKSMIQNRYREAGF
                     VPNVVQRSNNLNTIESLVSSNMGISILPSFCVTDTMIERDIAIVPIKEVENRIEVVVV
                     WNKKNTNPALEKFISII"
     gene            52975..54363
                     /locus_tag="PC700_000214"
     CDS             52975..54363
                     /locus_tag="PC700_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2579542.1"
                     /translation="MEDKFYAKGNGNNGYIKNLEVCSFNNLDGTCGMFQMALYKRDEK
                     YYLYGCCFGGNKKNGVMISDITDPYNPQFIKHFQMLDPKEYPTTTTPKIQIADDLMIV
                     AMSCGSGPGALVDQAKLANIKCEAGIRIYSLKEDPLNPKFLGYWDCGLKHVMGVHRFM
                     YNGGRYVHLSSDCVGFEGLIYRVIDIINPNNPVEIGKWWRPDQYADGYPNRTFDAGAP
                     HCPEFMDKGWLHGPPFVRDGKAYCGYGGAGLVVLDVEDLTRPRCLGELPFMPAFSSRL
                     AGARTHTALPLPGRDLVVVQNEGERFQFFKPDNITDVQAMNNIHMVDVSDPTKPTLIA
                     QFPYPEVPKDFPYPNFNVAGLGKAGPFGPHNLHEPMDNKPWLEQRGDRVYCCYFHAGL
                     RVYDVSDPYYIKELAYFIPPNPNKTPEESYFPGFPGPRLAVTEDLIVDDRGYIIIDAL
                     DDGFYILKMKED"
     gene            54391..55344
                     /locus_tag="PC700_000215"
     CDS             54391..55344
                     /locus_tag="PC700_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDF2579543.1"
                     /translation="MKILGISFGTKNGNNDTMCKVALKGAKEAGAEVEFIQMSSINIK
                     HCTGCCACVKTLLSGKGSMCVLKDDFEWLLDKMKDADGIVVSDPIFEEGASGLFHTIM
                     DRFGPRADRGNNIIGTKVAEAIGGKIPDSRMLNDKVISFMGIGGSDWGTRVQCEHAML
                     ALIPMWKVIDNAWFPWAKELVMDDTRLVQVHQIGLNIVEAAKDFEKASYQGEEGVCPH
                     CHNRLFYLESGTKKAICALCGIVGELDTISGKTIFSFPEEQLGHAHDTLPGKFIHGDD
                     IKKMEGHYAEVRKTQEFKERKTSYNFIEPLTKEKQAGVSLG"
     gene            55404..55628
                     /locus_tag="PC700_000216"
     CDS             55404..55628
                     /locus_tag="PC700_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2579544.1"
                     /translation="MNEKPSFKQIEIILAVIFLVLTYYKIGLTPAFIVIFIPLYLSYF
                     AVSMIVNQIAIKLLEIRQAREATNYNIGNG"
     gene            55621..56133
                     /locus_tag="PC700_000217"
     CDS             55621..56133
                     /locus_tag="PC700_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HDF2579545.1"
                     /translation="MDRKKKEILTLSMGIALLPPLWAVLAPYIGIKTGAVALICAGLY
                     VTNGNKQKDGLKIMFGFWCGDLWAVLAILIMGYMNFNQNLELFLTLSILGFFAVVIAS
                     LFEKIIFLPSWLCGWAIGLTIMTGENIINLQDICIQIAVAMAVGVWYVGAGVDLFTIY
                     ILSKDKKKGN"
     gene            56165..57019
                     /locus_tag="PC700_000218"
     CDS             56165..57019
                     /locus_tag="PC700_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HDF2579546.1"
                     /translation="MAMGFEFKIMRSLIYVGLAKEEYRPKLMDWLYRHHIPDSISTFG
                     PYCTKYAFYQAYPTPNEGERFGARKMQLTEHYWLVDEHMPEMANRIMTEYMPMDVLRW
                     QGCIPDVENKRVHENAESGDAGRAVGGDNGCPPFIFAFVPINWEEDFRGKGRTVQDGP
                     NYRWQFMIKYPDGISKEEGEKWFYDEVVPYFTNCCYVNRFVSSKIMINYGATAFDRVS
                     ELWFEGEEEWYKAVVEETKSFIKKPEWAQEEEFPYLKPQFNIASVFLGDIATMDAYSQ
                     YRGYIPMR"
     gene            57586..59673
                     /locus_tag="PC700_000219"
     CDS             57586..59673
                     /locus_tag="PC700_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2579547.1"
                     /translation="MVVDKEIVSILQILLNSTLITTNGLQEESNSSKRQITYRINKIN
                     DMLKVKNVPLICLRADKDIIVRKETKKAIKEILEKNYSKNTYYFSKTERLLYMYLMLF
                     INMEDISINHFINSIKVSRSTVNLDFKDLIPELEKKNIKVKNNRINGYYIVGNEMEIR
                     RVLIKNIIETLSNEKSSRVFDIFIKEYNLDSFEEAKKIILKLIKKYEITFVEDRLIEF
                     IYIFIFLKARMYSTRIVTQENVDIPNISVMSSMKEYKFAKELLEVYGSEDKIKPYNIM
                     YISAWILGISVGNYEEDTEDKDVISKIVNKMMTKFGYLSGVYYISQEKIFKQLYSHFR
                     PAYYRLLFKLPIYNPICEKVKEEYKLVYRIVSNAMKEFCGLFGEEIQEEELAYLTMHF
                     ATLFSNKKEFDGFRKRIALIVCSNGVGSSAILHAELTNLFPDLHFIPMSESVGLENIS
                     EPVDIVFTTNYNAEGLKTDVPVIKVSPVMTTKEKYKITREVYIQLGDIYLRQPKIDEV
                     MDIIKKYANVTSESILSSELLAYFTQIENFTSKEGEGPMLSEITNETLVKIKVKAKNW
                     EEAIRQSASVLVENNKVTEGYVDAMINSAKASGPYIVITKHVALPHARPEAGSKEIAI
                     GIATLDSPIEFGNNENDPVKYVFCLSAIDNNSHLRAMSELVELLEEDEFFKVLDSAKE
                     AKEIIDFIKTHEL"
     gene            59688..59975
                     /locus_tag="PC700_000220"
     CDS             59688..59975
                     /locus_tag="PC700_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2579548.1"
                     /translation="MYKILIACRAGVGSSLMLKIKTQQIIKENNFPIEVEHGSLDSLN
                     GFNGDAVETLIDVAEELKNKNLKFDIIGIHNIVDRNEIKTALEKFLASKEG"
     gene            60002..61273
                     /locus_tag="PC700_000221"
     CDS             60002..61273
                     /locus_tag="PC700_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HDF2579549.1"
                     /translation="MEFIISLLSTPAVLLGVVACIGLILQKKSAVEVFTGSSKTLIGF
                     LIFGIGASAMTGALQNFNKLFQHGFGITGVIASPEAATALAQGTYGFAVSCTLILGFI
                     LNLVFARITKFKNIFFTTGHSLFFSCVLVLIMKAHGFDNTITILIGGTILGFMSAALP
                     QFCQPFMRELTGGDEQAIGHFNMIGYGLSGYIGRLFSKHKDDTTETIEFPKWLSIFRD
                     FLMGLSIVMLILFYIATLKAGQAFTQEIAGSTHWLVFPLIEAFTFVAGMSILMSGVRM
                     FLAEITAAFVIISEKYIPGSRPALDVPTVFPYAPNAVIIGFISAYAAGLLAIAIMASF
                     PSIFPVVIIPAAHICFFSGGTAAIFGNTSGGWRGAIAGSFVVGLLLAFLPVILYPVYG
                     TLGIEGATFPNIDYNVVGSILHNILQFIKPI"
     gene            61331..61930
                     /locus_tag="PC700_000222"
     CDS             61331..61930
                     /locus_tag="PC700_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF2579550.1"
                     /translation="MENTLSEFLDNISNELSEFIASIDETSINKACELILEAEKNHGR
                     VHVTGIGKPGHVSGYISSLLSSTGTSAYILHGTEAVHGSSGQVVEGDVVIAISNSGET
                     QELKATLKTLKVNGAKIIGVSGNESSFLKNISDIFLFAGVKQEGDCLNKAPRASILAE
                     TIVLQSLSVVLQYAKGLNTQQYLKWHPAGSLGKSIREGI"
     gene            61935..62840
                     /gene="dapA"
                     /locus_tag="PC700_000223"
     CDS             61935..62840
                     /gene="dapA"
                     /locus_tag="PC700_000223"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDF2579551.1"
                     /translation="MKYKGIICAMITPFDEKQNINSQATCKLIDYLIEKGIYGLFILG
                     TNGECHVLTDDEKVEFAKIVINHTNNRVPVFVGTGGNSTREVIDLSKRMEAIGASALS
                     IITPYFVTPTQQELILHYKTIANSVNLPIIMYNMPGKTGINIEPESVRELSKIKNIVG
                     IKDSSGKLDNMKAYIEVTQNENFSVFSGSDSLILDSLKAGSQGAVAATTNFLTEIGIS
                     IYENFMKGDLEEAQKAQSSIEELRRILKLGTIPSVMKKTIVLNGINVGTARLPVTEPT
                     GEVLEEIKRVVENYRTILNKSSENR"
     gene            62946..63578
                     /locus_tag="PC700_000224"
     CDS             62946..63578
                     /locus_tag="PC700_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketohydroxyglutarate aldolase"
                     /protein_id="HDF2579552.1"
                     /translation="MKKMNITKRMSECGALAIVREENLNRACEIAEGCIKGGITVIEM
                     SYTLNNAGEIIQGLNKKYGEILCVGAGTVLDSETARHAILHGAQFIIAPNYNEGVAKI
                     CNRYQIPYAPGCTSLTEAVDALSLGATFIKAFPISDFYGPKLVKVFKTPIPDMPVLAS
                     GGINLENLHIWLENGVDVCGFGGLLTKGTIEDITENAKKIREIIKNIREI"
     gene            63993..66164
                     /locus_tag="PC700_000225"
     CDS             63993..66164
                     /locus_tag="PC700_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HDF2579553.1"
                     /translation="MKKIIYVLFTIELLIVSLLGLNIVKDNEINNILYNDTTSISVMF
                     KDYKKLNKNYSKWIKDIADENNVAISKYVFNNNEKLSIYTTDTSLNNNIKLKSGKFPN
                     TNSNEFISNINTNNSNNSNQVGKFSTMSKDISILIRDFGKISEVGESGILYISTQNED
                     TINKILRELNVDRSIFVARLHDRYVNSNLYLNPSIIRDLILIILCFLATIIHYSISVS
                     REASILRLNGYSKLDIIARVIKNIFRIMILSSVTALLIYIIYILKLNIGVRACLYFAM
                     FSIICILINILISILIVGFNSRSSKYVLSLNGKKSYGFVNIMHFTLKIVFVSFLILSI
                     NNCILNYNMLQTQLGNLSEWDKAKNVYNLTLKDTGEQSLGKEEVIKNAKIKGFYKNLV
                     EEKDAFLIDSTNFEKLEDGSYMYEINSKGKNPEVSQYGKNIKINKNYLKVNPIYSNGK
                     EIFNLIDYDDKTINLLVPKKLQVHENEIKKEFRELFYFEKIEVENMYNKELNRELNKT
                     KKSDLNVNIIYVDNNQSYFTYNSLVMDDNRNLIDDPIAIIDIDNIDDSFYLSYISRCV
                     YFNSKKLDALADISNIIEAQGVEAHIQSLHAVYNEYGLEINKLEKYLNSEIFTIIIIA
                     ISNLMITYNIVASYYERNKYKLYIKKIFGYSTTARSMLLIVSLILTNIIPIGIISTRV
                     DLSNNIILFGLLILVIEVIVSIVLDKIISNSSFNKIIKGEH"
     gene            66169..66786
                     /locus_tag="PC700_000226"
     CDS             66169..66786
                     /locus_tag="PC700_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2579554.1"
                     /translation="MISLINISKKFKDKSIIDKFNLEISKGEFVAITGNSGSGKTTLL
                     NIMGLLEKPNSGDIIINNIKNPNSKQIRILQRDFYGYLFQNYALIENENVENNLKIAL
                     KYQKNINHNEKINYALESVGLRKYNKKKIYELSGGEQQRVALARVILKECEVIFADEP
                     TGNLDKKNRDIVFDILKNLNKNGKTIVFVTHDLELSEQADRVIKL"
     gene            66975..67319
                     /locus_tag="PC700_000227"
     CDS             66975..67319
                     /locus_tag="PC700_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HDF2579555.1"
                     /translation="MENLPNCPKCNSEYTYEDGTLLVCPECSHEWALGLGAEEDSNII
                     KDVNGNVLNDGDSVTVIRDLKVKGSSSSIKIGTKVKNIRLIHDSSDGHDIECKIDGFG
                     AMKLKSSVVKKA"
BASE COUNT        25674 a         8140 c        11258 g        22501 t
ORIGIN      
        1 atataaatta acctactgtt taattttcaa tgttcgtgtt tatttcttgt tccttttttc
       61 aaggacaagt aatactatac catctttaaa acagtaggtc aatacttttt ttatattttt
      121 ttataaattt ttttcatatt cttttttcag cttctctact acctttttct ctagtctaga
      181 aacagtcatc tgtgatatcc ctagttcttt agctatacta gattgtgttt tatcaaagaa
      241 aaatctatct ttaaaaattt taacttctaa ttcatttaaa gtttcaataa acttattaat
      301 aaaatcttca tactctatac ttccaaagtt accttcttct tttccaatct tatcaataag
      361 agttatatct ttatcctctg aatcatcatt acttgatgaa tccaaagaca ttggttgata
      421 tccataagaa gcctccatag cctctaaaac atcttcttca ctaacgccta tataatcagc
      481 tatatctttt acctttggat gcttcttatt ttcttgttct aatttaatct tcgcatcact
      541 tatctttttg cttaattcct gtattcttct tgggactcta agtgtccata ccttatctct
      601 aaaatacttt tttatttctc ctactatagt tggtgtcgca aagctggaga actcaaatcc
      661 tttctcaaca tcatatctat caatagcata aattaatgcc aatgaagcaa cttgatatat
      721 atcttcaaaa tcaacacctt tgtttatata cttcttagct aacaatcttg ctaaatacag
      781 atgtctttca ataagaatat ttctaatgtc tacattttta tttttattat ataaattaaa
      841 tagctctttt gtatccatat ttaggtaatg tgtagcatta gctacatttt tcattaaata
      901 tcaactccta aatatttagt catttttatt tttgtccctt gattatgttc tgattcaata
      961 tctacatcat ccattaaagt tttaattata aacaatccaa gaccactctc ttttggattc
     1021 tctaaatctg gtgtacttat tgagctaaca tcatatcctt ttccattatc ttgaatttct
     1081 atattaattg cattatctaa aatattaaat actatattaa acacatcatc tttactgtgt
     1141 tttattgcat ttgtacatgc ttcagatact gctaccttta tatcttctat atcatctatt
     1201 ggaaaaccca tcttattggc aattccagat gctgttaatc ttattatact aacataatct
     1261 ggatttgtag taatttccat ctttatagtc tcacaagcca aagtttatcc ctccacttta
     1321 aatattttat ctagacctgt tatagtaaaa atctttttta cgttactctt aggatttact
     1381 atgtatatct ctttttcatt tatttttaat ttttttaata cacctatcat agctcctaag
     1441 cctgttgagt ctatataatc taaatcttta agattaatct ttacatctaa catatttttt
     1501 tctattaaag catgtaaatg ttcctttagt ttatctgctg tagaaacatc aagttctcca
     1561 tcaagacaaa cattccaaaa tttattttgt gaatctaaat ttgaatcaat attcatagac
     1621 atgtaaaaac ctcctatttc cttttaataa ttttaaatat atttcctata ctcaatattt
     1681 tattaattac ataaactata tcatctattc cctttgatat tgatgatgca ttttcaacaa
     1741 tatcttgtat atgtctttca ttataatcta caatcttata tgccttttca acaaccttat
     1801 ttatgctgtt tagagtttta gctaaataaa ttgctactat aagagcagag cttcctacta
     1861 acactgctcc aatttgccac ccccatgcat tcattagtta acatctccct catcatcaaa
     1921 gcttttactt attactatat cttcttctga aatctttata tcttcttcat tattatcatc
     1981 gtccactaaa tttattattc tctctttaac atcattaaat gtttcatgta gaacttcttt
     2041 tggattttct ttgacatcat taaacttctc ttttgtttct tttcttagtt cagaaccttt
     2101 tttaggtgca aaaaacatac caaatataaa ccctaaaata gctcctaata ataaaaatcc
     2161 acctttttta caacttcttt tattgcacat aacaattact cctttcaatt ttaaattttt
     2221 atctaactac attatacaaa aagaatagga tatttatcct attcttcttc aatatttatt
     2281 ttggcaatcg aaactacatt gacttcatca ttcgtcttca ttaatttaac accacttgta
     2341 actcttccaa acagtgatat ttcattgact cttattctaa taagaactcc atcagaattt
     2401 ataatcatta tctcatcatc ttcgttgacc atatctgcac ctacaattgt accagttttt
     2461 tcagatatat tataagtttt aattccttta ccagctctta tttgacttct atactcttct
     2521 atatttgttc tctttccaaa accattctta cttacaacta acacatctgt acctttactg
     2581 caaaggttca tagacacaac aaaatcttct ttacttaaag ttataccttt tacacccatt
     2641 gctgttctac ccatatatct tatatcattt tcatcaaatc ttatagacat accttcttta
     2701 gttactaaaa gtacttcttg ttttccatct gtaagttcta ctccaataag ctcatcatca
     2761 tctcttaaac ctatagcaat aagaccagat ttatttatat ttttgaactc ttctctctta
     2821 gtctttttaa caataccttt tttcgttgca agtaataaat attcattttc atcattacca
     2881 tcaataggta ttagagtagc aattttctca tctgctgaca attgaagtaa attcactata
     2941 gcagtacctt tagcctgtct cttaccttct ggtatttcat atgcatttaa tttaaatact
     3001 ctacctttat ttgtaaagaa taatagtcta ctatgagtag ttgtagttac caagtgcctt
     3061 acaaaatctt cttctctagt tgtaagtgct gaaatacctc ttccacccct tttttgactc
     3121 ttataagtat cagatggaag cctttttata tatccaaagt gagtaagagt tattgctatt
     3181 tcttcatcac ttataagatc tctcatatct atttcgcctt cagcatgtct tatttctgtt
     3241 cttctctcat cagagtaatt ttcttttatt attgtaattt cattctttat tacatttaaa
     3301 agtaatcttt catcagctaa aatttctttt aatctattta tcttcttgat taaatcttca
     3361 tattcagctt ctatcttatc tctttctaaa cctgtaagtc tttgaagtct catatctaat
     3421 atagcttgtg cttggatttc agttaatttg aatttttcta ttaaacctat cttagcttct
     3481 tgcccagtct ttgaagctct tatcaagctt ataacagcat ctatattatc taaagcaatc
     3541 tttaatcctt ctaaaatatg tgctcttgct tcagctttat ttagttcaaa tttagttctt
     3601 cttgtaacaa catcttcttg atgtttaata taatgatata atatttgttt aagatttaaa
     3661 actcttggct gaccatctac aagtgcaagc attattatac taaaagtatc ttccatttga
     3721 gaatgtttat acaaattatt taatactata ttagcattag catccctctt taattctata
     3781 acaattctca taccatttct attactttcg tctcttaggt ctgatatacc ttctattctc
     3841 ttttctttaa ctaactctgc tattctttca accagtttag ccttatttac ttgataaggt
     3901 atttctgtaa ctattatttg ctgttttcct tttggtagtt cttctataaa agctctagac
     3961 ctaactttaa cttttcctct tccagttctg tatgcttctg ctatactttc ttttcccatt
     4021 ataattgcag ctgtagggaa atctggtcct tgaacaaatt ttattaaatc atctacacta
     4081 cactctggat tatctatcaa atatacagtt gcatcaatta cttctgctaa attatgtgga
     4141 ggtattgaag ttgccatacc aacagctata ccatttgagc catttactaa taaattagga
     4201 tatctagctg gcaatactga aggctctttt aacgactcat caaagtttgg tttaaagtct
     4261 acagtttcct tttcaatatc tctcagtagt tctaatgata atttactcat tttagcttct
     4321 gtataacgca tagcagctgg tgaatcccca tcaacagaac caaagttacc atgaccatct
     4381 actaaaagtg ctctagttga aaaatcttgt gccattctta ccatagcata ataaacagca
     4441 atatctccat gaggatggta cttacctaaa acgtccccaa caatacgagc tgacttcctg
     4501 tatggtttat ctggagttaa atttaactca ctcattgaat ataatattct tctatgaact
     4561 ggctttaaac catctctaac atcaggaaga gcacgtccag ctataacact cattgaataa
     4621 tcaatatacg attttttcat ttcttccgct atttcaatag ggagtatttt gttattttct
     4681 tccatgtata ctaatcccct ctctctatat atctaaattt ctaacaaatc ttgcattttc
     4741 ttctataaat tcttttcttg gagcaacctt atcacccata agcattgaaa atacttcatc
     4801 tgcaattgca gcatcttcta cagtaacttg taataaagtt cttgtctcag gattcatagt
     4861 tgtttcccat aattgctctg cattcatctc tccaagacct ttgtaccttt gaagttcaac
     4921 accatttctt ccaatttcat ctaataagat atttaactct ttatctgagt aaacataatg
     4981 ttccttcttt tgctttgtca ctttatataa aggtggctgt gctgcataaa catacccctc
     5041 atctataaga ggtctcatat atctaaagaa gaatgttaat aataaagttc ttatatgggc
     5101 tccatctaca tcagcatcgg tcataattat aattttatga tacctagcct tatctatatc
     5161 aaaatcttct ccaataccac aaccataagc tgttatcata ttttttattt catctgaaga
     5221 tagtatccta tctagtcttg atttctcaac attaagtatt ttccctctta atggaagtat
     5281 agcttgacta tttctatctc taccttgttt agctgaacct cccgctgaat ccccttcgac
     5341 taagaatatt tcacttttag atggatcttt ttctgcacaa tctgcaagtt ttccaggtaa
     5401 agatgtactt tctaatacac tttttctcct tgttaattct cttgcttttt ttgcagcttc
     5461 tctagctctt tgtgctctta aagctttatc aactatgatt cttgctgttg atgggttttc
     5521 ttctaggaaa gaccccagtt catcaactgt tacactatca actatacctc tcataaagct
     5581 attacctagc tttgttttag tttgaccttc aaattgaggt tctggtaatt taactgatac
     5641 tactgctgta agaccttctc ttatgtcttc accaagtagg ttaacatcat tttcctttaa
     5701 aaatttattt ctcttagcat aatcatttac agttttagta agagctgctt taaatccact
     5761 taaatgagaa ccaccttcgt gtgtatttat attgtttgca aaagaatata tgttctctgt
     5821 ataaccatct gtatattgca tagctagttc aacaaaacaa tcatcaacct ttttatctat
     5881 ataaactatg tcatcatgta taccagttct agatttattt aaatacttaa tatattctac
     5941 caaaccacct gtatagtgaa attcttttct tttttcttgt ccttctcttt tatcttcaaa
     6001 tactattttt attcctttat tcaagaaaga aagttctcta agcctatact ccaatgtttc
     6061 atatttaaac tctatttcat caaatattgt ctcatctggc ataaattgaa tcattgttcc
     6121 tttatcttga gattctccaa ctacttcaag cttagatgtt ggtaatcctt tctcatatgt
     6181 ctgcttatat atctttccgt ttaaataaac ctctgctacc atccattttg acaatgcatt
     6241 aactacagaa acaccaactc catgaagacc tccagatact ttatatcctc cgcctccaaa
     6301 ctttcctcct gcatgaagat tagttaatac tgtttctaaa gttgatttac cagttttagg
     6361 atgtatttca acaggtatac ctcttccgtt atcttttact aaaacactgc catcttcatt
     6421 aatagataca tatatgtctg aacaatatcc ttgtaaagcc tcatctatac tattatctac
     6481 aacttcataa actaagtgat gtaaacctct agggcttgta ctaccaatat acatacctgg
     6541 tctttttcta acggcttcta atccctctaa tacttgtatc tgacttgcac cgtattcttg
     6601 tttcatttaa tttcctccat tctctatgct aataacatcg ccattttcaa tattaaatat
     6661 tgtaacattt ttttcatcaa atattttttt atgtgaaact tcagcacttg ttataaacat
     6721 ttgaacattg cttaaattat ttactaaaag tttttgtctt gcttcatcta actcactaaa
     6781 aacatcatct aaaattaata ctggatattc ttcaacttca ttttttatta attctatctc
     6841 tgataacttt aatgatattg aagcagttct ttgttgacct tgtgaaccaa aaagtcttgc
     6901 atctaaatca tttatgaata tatttaagtc atctttgtgt ataccatatc tggtagtctt
     6961 agattctata tcattaggtc tattcgatga caacttggct aaaaatttat tatatactgt
     7021 atctatagta tcttcatcag ttatatttat ttgattttta tatcgaattg ataatctttc
     7081 aactccatta gtcaaattca tatgcatatt ttcagatata ttagctatct tttttataaa
     7141 gtctctacgc aaaatataaa tataacttcc atactttgct aacgtatcat catatacatc
     7201 taataaagct tcatcaacat gtatattttt aagcacccta ctcctttgag aaagtgtttt
     7261 attataattg gtaagatatt tataatattt aggtataatc tgactaatct ctttgtctat
     7321 aaatgccctt ctttctttag gaccctcttt aaccaacctt aaatcttcag gagaaaatat
     7381 aacaacattt aagttgccaa gtagttcttg tatcttttgt aaaggaactt tatttattct
     7441 tatccccttt ttatcttttc caataattag ctctattaga ctatatttat tatattttga
     7501 aaaacttcct ccaatgtata agttttcact attaaatcta accatttctt tatctttgtt
     7561 agtcctaaat gattttccaa aagaaagcat ataaatagat tctactatat ttgtctttcc
     7621 ttgaccattt ttaccaacaa gtagatttac tttcccatta aattccagat gcaacttttt
     7681 ataatttcta aaattaacaa gttgcaaact tttaagtttc acaaaaagtt ctcctttttt
     7741 tacaatactt ttattttagt tccttctatt ttaactatat ctcctgattt tatctttttt
     7801 cctcttctta attctatttc atcatttact gtcactaaac cttcttgaat taaaaatttt
     7861 gcatgacctc ctgttgatgc aatctctgct aatttaagaa attgatccaa ctttatatat
     7921 tctgattcta tagttatttc agtcataata gttcctcctt gtcactattt taaacaaatt
     7981 cctattgata cactaaaagt atattatacc ataaatatgc ttataaaagg agttgtctaa
     8041 gattagaaaa attttctaca aaagacaact ccttcatgtc tgctatatat ttgatgatat
     8101 tcttactgga agcagtaagt aaatataatt aacatcatct gttggtttaa ttatacaagg
     8161 atttacattt gtagtaaatt ctataaatat ttcctcatta tctatatttt tcaaaccttc
     8221 gataaaatat ctagaattga aagctatatc taggtaatct ccatctaaat ctatttcaac
     8281 ttcttcatac acatttcctt tttctgtgtt agaagtaatt gccattactt tatctcttat
     8341 agataacttt ataagattat ttttttctga ttgagacaat aaagacgctc tctctatact
     8401 atttaaaagt tccttagtgt ttaatttaac tctgctgtta tgttctcttg gcaataattt
     8461 tttataatca ataaaatctc cttcaagcaa tcttgtaata attttcgtat cattaattat
     8521 aaaaatagca tttttttcgt taaacccaac tttaacatta tcctctccag aaagcaaact
     8581 atttacatct ataagtgtct ttccaggaat tatgactttt atattttcag ttaaactatc
     8641 aactgaacaa ctttttacag ctaatctata tccatcaatt gctactaaat ttaagtttct
     8701 atctacaatt tctaaaagtt ctcccattaa aacaggtttt gtttggtcct gtgagatagc
     8761 aaatacagtt tgtttaatca tattttttag aatttcttga ggtatgctat ataaatcttc
     8821 ttcatttaat tctggtaatt taggaaattc ttttgctgca taacctttaa ttttaaatct
     8881 tgaatttaca caatttatgt aaatattatt ttctgaatca gtttctatct caacaaagga
     8941 atctggtaat tttctaatta tatctccaaa tagccttgca tctacaacaa catcaccttt
     9001 ttctatgatt tctgcttgta cataagtttc tattcctatt tcagcatcat atccagtaag
     9061 ttttaattga ccttcttctg tagatataag tatacctttt aataattcta ttgttgtttt
     9121 tccattaata gctttttgag caattccaat tctatttgct aggatttttt gattacaaat
     9181 tattttcaat gtggataacc tcctctggct ttttatatat aaaaataagt taaaatcata
     9241 gtagtaatag tagtaggtgt tgttgataat gttaataagt atgtataaca ttataaatac
     9301 aaagatatta acatgtgtat aagatgttaa tatctttgtt attttagact tgataatttg
     9361 ttaataattt ttgttcaaaa aaagtattaa caggttattc aagagatata aacatacaat
     9421 tgtgtataaa ttatcccttc aaatctgata taattttgtc tattttagtc ttaatttctt
     9481 cactttcttc tatatcctta gatactttgt catgagcatg aattactgtt gtatggtctc
     9541 tacccccaaa ctcttcacct attttaggaa gggataaatc agtaagctcg cgcgttaaat
     9601 acatagctat ctgtcttgga taagctattg aacgtgttct ttttttagaa ttaaaatctt
     9661 ccatctttaa attgaataca gaagatactt tttcttttat tctaagaaca tttatttctt
     9721 catttttaga agttgttata atatctttta aagcttctgt agcaagatca aaagatatta
     9781 ccctattgct aagagaagaa taagcaacca ctctagttaa tgcaccttct agttccctta
     9841 tgtttgactt aatatttttt gctatatatg acataacctc atttggcaca tctatacgtt
     9901 ctagttgagc cttttttcta agtatagcaa ttcttgtctc aaagtcaggt gcttgaatat
     9961 cagttataag tcccatttca aatctcgatc ttagcctatc ttctagtgta ggtatgtctt
    10021 ttggcggtct atcactagat ataattattt gtttgtttgc ttcatgaaga gtattgaatg
    10081 tatggaaaaa ctcttcttga gttctttctt ttcctgctat aaattgaata tcgtcgatta
    10141 gtaatacatc tacgtttcta tatttattcc taaattcttc gtttttatcg tcttttatag
    10201 agtttataag ttcattagta aatttttctg aagaaacgta aactactttt gagtcttttt
    10261 tttgactaac aatatgatga cctatagcat gcattaggtg agtcttacct aatccaactc
    10321 ctccatacaa aaaaagtggg ttataagctt tagctggaga ttctgcaact gcaacgcatg
    10381 cagcatgtgc aaatctattg ctattaccaa taacgaatgt atcaaatgtg tattttggat
    10441 ttagattggg gtaaagaact ctataattta aatctgtgct atcagaattt aaatcagcaa
    10501 cctctttttc gctcaataca aatttaatat tatatttttt taatgacagt tgactaatag
    10561 catcttcaat taaatgtaaa tatctattct ctaagatatc tttattaaaa tcactaggag
    10621 ccagtaagat taaatcatta agatgtattt ttaatggtac gatgttttta aaaaaggtat
    10681 taaaacttac tgaagttaag tcacctttta ttaattgtag ggttttgtcc cataaagaaa
    10741 ctatatccat aatataaacc tccgaacaga ataattaata ttttccacaa agttattaac
    10801 agaaaagcaa aaatatatat aaattttaca gaataactaa atttatggat aatggtgttg
    10861 ataaattaat aatagtataa attattaaaa aaatatgaaa agatataaac aatatgtaga
    10921 taaagttata aataacataa acatacaata aatggtgatt ataactagag tattgtcagt
    10981 aatcttaaaa gttatacaca aaagtatcca caagctgtga ataatttcta ataagttgat
    11041 aacatttatg cacattatca acaagtatat ttataatatc aaaatatatg tcaaacttca
    11101 atgatttgta actaatttat ccacaatatt aactaattgt tgataaaaat ataaacatgt
    11161 taaaattact ttatttgtgt atataattga aaaaaataat ttagaataaa attaaataga
    11221 tttttgctag tattttcaat aaaaaaaata cataatatat atatggtaga ttttgtgata
    11281 taagcgtccc aaaaactcgt tgacaacagt agtaaaagta tttataatta tatgtgttat
    11341 gtttactttg agggtaaagt tattattttt ataaacggaa aataaaatct gaaaggcggt
    11401 gcaaaataat gagtaaaaga acttatcaac caaaaaagag acaaagaagt aaagaacatg
    11461 gtttcagaaa aagaatgaag acgtctaacg gaagaaacgt gttaaagaga agaagagcta
    11521 aaggaagaaa tagattaact cattaattac aaaagggccg ctgcaaaggt ggcctttttg
    11581 tgtaaataat tacaattcat agctgtgttt atggaggtta actatggact ttaataggac
    11641 taaagggttg aaaaaagact ctgattttag aaaagtatat aaacacggca aatcttttgc
    11701 aaacaaatat ttagtaatat atatacttaa aaataaatca gattatagta gagtaggtat
    11761 ttctgtttca aaaaaagtag gaaaagctat tactaggaat agagtaagaa gattaataaa
    11821 agaagcctac agattaaata ttgatgaaaa aattaagcct ggatatgata tagtatttat
    11881 agcaagggta tctagtaagg atgctacttt taaagacata gacaagtcta taaaaaattt
    11941 agttaaaaga acggatattt ctatttaatt gtatgctata aatatggagg ttaatgactt
    12001 tgaataaaat aatttatata ttaaaggaaa taagtaggta tttatcgaat ttatgtattt
    12061 atttagtgag attttatcaa aagtatatat ctcccttaaa gggacctacc tgtagatttt
    12121 atccaacttg ctcacaatat tcaatagagg cgtttaaaaa atatggattt gtaaaaggta
    12181 tgtatttaac gataagaaga cttttaaaat gtcatccttt tcatccagga ggttacgacc
    12241 ctctaaaata aaagagagta actattagga ggtataaaaa aattgaatat aataggaaat
    12301 ttcttaggat tgattcttaa ggtaatattt gaatttgtta atcattatgg tatttctata
    12361 atacttttta cgatacttgt aaagattatt ttattaccat taacaattaa gcagactaag
    12421 tcaacaaagg caatgcaaga tatacaacct cgtataaaag agatacaaga aaaatataaa
    12481 aataagccag aaaaacaaaa tgaagaaatt gttaagttat atggagaagc taaaattaat
    12541 ccactatctg gttgtttacc tttattaata cagtttccta tattaatagg attatttagc
    12601 gttttgagag aaccagttgc tcatggcgtt tttgcaaaca aagctgcatt tttagcagct
    12661 gacaatgggt ttttatggat aaaaagctta acatcaccag attatgtatt agcagtattt
    12721 tcaggagcaa gtgcatatgt catgcaaaaa gtaatgacac ccaaagatca gttacaaggc
    12781 tctatgaaag taatgacata tgtaatggca ggtatgtctt tctattgggg atttatattc
    12841 ccagcaggac ttacattgta ctggacagta agtaacttat tctcaatagc tcaatattac
    12901 ttaataatga atcctttaaa agctaaactg gctgctaact ctaaggagga aaaagttaat
    12961 gagaaaaagc ctaaaattaa taaaaagtaa gagtaaagag gaagctataa gtaaagctat
    13021 agcagagttg aatttaaaag cagaggatat agaagtagaa atacttgaaa atccaagtaa
    13081 agggttttta ggttttatag gtgctaaaga tggtacttat gaaatttttg ttatcgagag
    13141 ggaagtagat gtagctaaaa attttataga ggtaatgttg aaaaatgcaa atgttgatgc
    13201 caaagtcaat gtatctcaaa aagataattt aatcatggtt gatattgaag gcaaagaagc
    13261 tgcaagttta attggtagaa gaggggaaac tttagattct attcaatttt tgactggatt
    13321 agctcttaat aaaataaata aagattctca tacaagagta cttgtagata tagaaaatta
    13381 tagatcaaaa cgagaagaat ctttaataag atatgctaat aaagttgcta gagaagtagc
    13441 gaaaacaaga aaaactaaaa aattagatta tatgaatcca tatgagagaa gaataataca
    13501 ttcagctctt caaaatgaca aatatgtaat tacatatagt gaaggaaccg atccatatag
    13561 aagattagta attgagtgta aaagataaag tcaacctcct aaacattagg gggttttatt
    13621 ttttaatatt attaaatcat taatgattgg ttaaatgtaa acctatatgt aaaaaattat
    13681 gaattaagag gtgataaatt tgtttataga tgatacaatt gcagcaatag ctacagcacc
    13741 tggagaaggt ggtataggta tacttagaat aagtggagaa agagctttga aggttgcaga
    13801 agaaatcttt aaatctatgt cgggaaagag tattgaagaa tacaataaga gaacattaat
    13861 atatggaaat atattggata atgaaaatat aattgatgaa gttttattgg catatatgaa
    13921 aggaccaaac tcctatacag gagaagatgt tatagaaatt aattgtcatg gtggatttat
    13981 ttcggtaaag aagatattag aattaatact atcaaaagat gttagattag cagaggctgg
    14041 tgagtttact aaaagagcat tcttaaatgg aagaatagat ttatctcaag cagaggctgt
    14101 aattgatgtt ataaaagcca aaactgatat agctcatgaa gttgctcaaa atcaattaga
    14161 gggttcttta tcaaagaaga taagagaatt gagagataaa gtcactgaga tattagctca
    14221 tgtagaagtt gctatagatt atcctgagga agatattgag catataacat atcaaactct
    14281 taaagaaaaa actgatgaac ttaaaaaaga gattaaaaaa ttatatgata cagctgaaag
    14341 tggaaaaata cttagagaag gcttaaagac ggtaattgtt gggaaaccaa atgtaggtaa
    14401 atcttcacta cttaattcta ttttaggaga gaatagagca atagttactg atataccagg
    14461 aactactaga gatgttatag aagagtttgt aaacataaaa ggtatacctt taaaaattgt
    14521 tgacacagca ggtataaggg atactgatga tatcgttgaa aaaataggtg ttgaaaagtc
    14581 taaagaatca tttactagtg cagatttgat agtaatggtt ttagatgcat ctagaaagtt
    14641 gtcagaggaa gatatagaga tattagaaaa acttaaggat aaacaaacaa tagtgctgtt
    14701 aaataagaat gatttgaaac aagagattga agaagaaaaa atacttaaat atgtagaaaa
    14761 taatagtata ataaaaatat cagctttaca acaagaagga atagaagaat tgcaagataa
    14821 aatagagtct atggtatata aaggtagtat caaaaataat tctagtttag tagtaacaaa
    14881 ttcaagacat aaagatgcac ttagtaaagc atataaatct gcaacagatg ctttaattgc
    14941 tttggaacaa agtatgccat ttgactttgt agaagtcgat ttaaaaaata tatgggacta
    15001 tcttggatat attaatggtg atacagtaac agaggattta ctagataata tattccataa
    15061 tttctgtatt ggtaagtaat gtacaaaagt aaatttgttt ttattataga gatttttaaa
    15121 aaatttgaag tgtttctcat gaaacattaa ttttgaaaag aaaggaatta taaatgataa
    15181 agtttgaagc aggaaaatat gatgttatag tagtcggagc aggacatgct ggatgtgaag
    15241 ctgctttggc tactgcaaga atgggatata aaactttgat aataacaatg tctttggatt
    15301 ccatagcatt aatgccttgt aatccatcaa ttggagggac tggtaaaggt caattagtta
    15361 aagaaataga tgccttaggt ggtcagatgg gattaaatat agacaagaca tatatacaaa
    15421 gtagaatgtt aaatactgcc aaaggtccag cggtacactc tttaagagca caagcagata
    15481 aatttaaata tcatgaagaa atgaaaaaga cattagaaga tgaaccaaat cttgatatag
    15541 ctatggatga agttgtagaa atacttcatg aaggaaatgt tgtaatagga gttggaacta
    15601 aactaggatg ttcatttaaa tctaaagctg taatattggc cactggagtt tatctgaact
    15661 caaagatata tatgggtgaa gttgcatttt atgaaggtcc taatgcattg gggtatgcaa
    15721 aatatcttac agatagctta gttgagcttg gtcttagaat gagaagattt aagacgggaa
    15781 ctccagcaag agttcataga gatagtatag acttctctgt aatgtcttta caagagggcg
    15841 atgaaaaagt aactccattc tcatttatga atgaaaatat agaaaaaaaa caagaacctt
    15901 gttatttaac aagaactaca gaagaaactc aaaaagttat actggataat ttaaaaaggt
    15961 ctgctatgta tagtggagta atagaaagta ctggtccaag atactgtcct tctatagaag
    16021 ataaagtagt aagatttagt gataaaacct cacatcaatt atttatagaa ccagaagggt
    16081 taaatactaa agaaatgtat atacaaggaa tttcaactag tttacctttt gaagttcaat
    16141 tagatatgta caaaactata aagggacttg aaaattgtaa gataatgaga cctgcatatg
    16201 ctattgaata tgattgtgtt gacccaactc aactaaaaat aagtttggaa ataaagggtg
    16261 tcgagaactt atttagtgca gggcaattta atggaacttc tggatatgag gaagcagcag
    16321 ctcaagggct tatggctggt ataaatgcag ttagaaagat agaaggaaaa gagccgtttg
    16381 tattagaccg ttcagaagct tatataggtg tcttattaga tgaccttgtt actaaaggaa
    16441 ctaacgagcc ttacagaatg atgacatcaa gagcagaata tcgactatat ttaagacaag
    16501 ataatgcaga tatgagatta actcaaaaag gttatgatat aggattagtt aaaaaagata
    16561 gatatgaaag gttcctaaat aaaaaggctg ctgtagataa agaatttgaa agattaaaaa
    16621 atgaaagagt tacaccaaaa gaagtaaata gtttgttaga agaaaaagga gcaacaccaa
    16681 taaaggttgg aatatctcta tatgaatttt taaagagacc agaggttact tacgaattgc
    16741 ttgaagaatt agggaagggc gctggagaag atgtttcaag agaagttaaa gaacagtgtg
    16801 taataataac taaatatgaa ggttatatag agaaacaatt gaaacaaata gaccaattta
    16861 aaaaacttga gaataaaaag ctggatgaga aaataaatta ttcatctata gaaggtctta
    16921 gattagaggc tagacagaaa ttagatgata taaaacctat ttcaataggg caagcatctc
    16981 gtatatcggg tgtttctcca gctgatatat cagttttact tatataccta gaacaagtca
    17041 gaagaactag aggtggcaaa ggtgaataat atagaacttc tgaaaaacgg gattgaaggt
    17101 tttaatatag atacgaatga cagtatgtta gaaaaattta aaatctatag agagatatta
    17161 gttgaatgga ataaaaagat gaatcttact ggaatagaag atgaaaaaga agtgtatata
    17221 aaacattttt tagactctat ttcagctgta aagaatggat atataaaaaa tgggatgtct
    17281 ataatagatg ttggtacagg tgctggtttt cctggaatac cattgaaaat atgtttagat
    17341 agcttagaac ttactttgtt agattcgtta aataaaagaa taaacttttt agaggaagtt
    17401 agtagagtac tagagttaga taatattaca tttatacatg gaagagctga agattttggt
    17461 aaaaatgaag actatagaga aaaatatgat gttgctacgg caagagctgt tgctggatta
    17521 ccgattctta tggaattttg tgtaccattt gtaaaagttg gagggtattt tatttgctta
    17581 aaaggtccga atgctaattt agagctagaa gaatcacaaa aggctattga tgttttagga
    17641 ttggagtata ttgaaaaaat agatgtagag ttaccagagg tagatttaaa tcataatata
    17701 ttagttttta aaaaggtgat ggaaacacca gttaagtacc ctagaaaagc tggtagacca
    17761 gctaaaaatc ctattaagta atgaaaaaag taatgaaaaa attattattg gtaagcttaa
    17821 ttatttctta ggaaataatt aagcttaccg taaaaaaaca aaaataaagg aattattaaa
    17881 gtagatgcag aaataaattt gtatgtacat atagagaaca aaaataattt atatctaaaa
    17941 taagagaggt atgttatgga agataaaaga gttatggaga tacctataga agatgtagtt
    18001 ccaaatccat atcaaccaag aaaaatattt tcacaagttt cattggagga attgagcaat
    18061 tctataaagg tatatggtat aattcaacct ataacagtta gagcaaaaga tggcaaatat
    18121 gaactaattg caggagaaag aagattaaga gcagctaaat tagcagagct taaaaccata
    18181 ccagcaatta taaataatat gaatgatgaa tcttctgctg ttctagcgtt attagaaaat
    18241 ttgcaacgag aagacttaaa tttcattgaa gaagcaattg gttatgaaaa cttaataaaa
    18301 gaacatgctt ttactcaaca gcaattagca gaaaaattgg gcaaaaatca atctactgta
    18361 gcaaataagc ttagaatatt aaagcttcca aacgatataa aaatgaagct aatagagaat
    18421 aatttaacag aaagacatgc tagagcattt cttaaactac caagtgaaga tttaatgcaa
    18481 agtgtgttag acaaagtcat aaaaaatgaa ttgactgtta aaaaaacaga aaaattgata
    18541 caagatgtat tagaagaaac taaagttcaa gaagagcctg ataaaaaaca gaatataaag
    18601 ggtgctatga gtataagaat ttacataaat actataaagc aggcctttga tgctatatca
    18661 aatacaggaa ttgatgctaa gtataatgaa atagataagg gagattatat ggaagttgtt
    18721 gtgaaaatcc ctaaaaaata gagattactg cttaccagta atcttttttt gtaatataag
    18781 ccttgtaata taaattgatt tatagacatt atttatactt aaaaaacacc tattcaacat
    18841 ttaaaattaa tttaattctt aaagggggga acatcatggg caaagttata gctgtattca
    18901 atcaaaaggg tggagtcggg aaaactacaa caaatgtaaa tttaagtgct agcttaggaa
    18961 ctttgggaaa gaaaatatta gtattagatt tagacccaca aggaaataca actagtggat
    19021 atggtattaa taaaaacgaa gtagaaaata caatctatga aattatgtta gatgggttac
    19081 atattaaaga agcaataata agtactgaat ttgagaatgt agatgtagtt ccatcagcta
    19141 ctgaactatc aggtgcagag atagagctta catctaaaac taatagagag tatattttaa
    19201 aaaattccat aaaagcggta atagatgaat atgattatat atttttagat tgtccaccct
    19261 cactggggat gcttacaata aattgtttaa ctgctgtaga tagtgtttta attccaatac
    19321 aatgtgaata ttatgcttta gaaggtgtta gtcaattaat ggagactata aaattagtaa
    19381 aatcaagatt aaatgctgat atagaaattc aaggtgttgt tttaagtatg tttgatggaa
    19441 gagctaattt atcaatacaa gtagttgaag aagttaagaa atactttaaa ggtagtgtat
    19501 atacaacttt aattccaaga aatgtaagac ttgcagaagc tccaagtcat ggaaaacctg
    19561 taatttatta tgataaaaga tgtagagggt ctgtagctta tttggagcta gcagaagaat
    19621 ttatagattt agaagaggag gaatggtagt atggagaata agtctaaaag aagtaataga
    19681 ttaggtagag gtcttagtgc attgatacca gaaattaaag gagaaacttc agaaaaagaa
    19741 atagtcaaca tagatataga caaaatatat ccaaacgaag ttcaacctag aaagcaattt
    19801 gatgaagaaa agataaaagt attgtcggac tctataaaaa attatggtgt tttacagcca
    19861 atagtggtta aaatggatga aaataataaa tatatgataa tagctggaga aagaagattt
    19921 agagcatcaa aacttgcaaa taaaaatcaa atacctgcta taattaaaga tatagatatg
    19981 aaagatataa tggagatagc cttaattgaa aacttacaga gggaagattt aaattcaata
    20041 gaagaggctc tagcatataa gagtttaata gagcattaca atgtaactca ggaagaaata
    20101 tcagaagctg ttgggaagag tagacctcat ataactaata cactaagact tttaaatctt
    20161 ggacaagatg ttatagaaat gatagacagt ggtagaatta cagcaggcca tggtaaggct
    20221 ttattgagaa ttgcagataa agatttgcaa cttcaaatag cgaaaaagat agaagaagaa
    20281 gaactttctg taagagaagt cgaaaatata gctaaaaaaa tatctgaaaa taaacaagaa
    20341 gaacctaaaa aatctaaacc taaggatgta tttattttag atgttgaaga taaacttaga
    20401 aatatatttg gaacaaaggt gaatatttct aaaggaaaga agaaaggtaa aatagagata
    20461 gaatactata atgatgatga tttaaatagt attgtatcaa tgcttcttga atagaacaaa
    20521 aatataaaac agagaggaag attgttatga tttatttaga taatgctgca actacatatc
    20581 caaaaccaga aagagtgtat aatgctgttt tagattgtat gaaaaattat tgtgcaaatc
    20641 caggtagagc aggccataaa cttgctatga gagctgctag ggagatatat gatacaagag
    20701 agaatatagc taagttgttc aatgtaagta atcctatgaa tatagtattt acatctaatg
    20761 caacagattc tttaaattta gcgataaaag gtgtattaca agaaggagac catgtaataa
    20821 caacgtctat ggagcataat tcagtaataa gacctataaa agcattggaa aaaaggggta
    20881 ttgaaaatac tgtagtaaaa tgcgattatg agggattttt agattatgaa gatttagaaa
    20941 aatcaataaa gagtaatact aaactgattg taacaacaca cgcatctaat gtatgtggaa
    21001 ctttaataga tataaaaaaa gttggagaaa tagctaaaaa acataatata ctatttttag
    21061 tagatgcatc acaaacagca ggtgtttatg atatagacgt aaatgaatgt aatatagata
    21121 tgttggcaat gccaggtcat aaatgcttat ttgggcctca aggaacaggt atattatatg
    21181 taagagaagg gttgaactta aatatattaa aagaaggtgg aacaggtagt aaatcagaag
    21241 aaatagttca accagagtta ttcccagata agtatgaatc aggaactcat aatactccag
    21301 gtatagcagg tcttaaccaa ggtatactat ttatatttga aagaggaatc aacaatataa
    21361 gacaacatga agaagaatta tgtcaatata tgatagacaa actagaggaa gtatcagata
    21421 taaagatata tggaccaaag gatagtaaaa aaagagcatc agtaatcgct ctaaatatag
    21481 gagatatgga ttctggagag gttactttct tattagatag tgattataat atagcaacta
    21541 gatcaggaat acattgttca ccactagcac ataccacttt aggtacttta aaacaaggtg
    21601 cagtaaggtt cagtatagga tatttcaaca caaaagatga gatagataaa gcagtggaag
    21661 ctttgaaaaa aatttcaaaa aataaatagc atgagattta ataatataga aataaaaaaa
    21721 ggggatattt caaaataaaa atttgaatca tctccttttt tgtacaaaaa agaaaagggg
    21781 ctcctaagaa cctctaatct atttgataat tgtttcaaga aattacttta agtaatctcc
    21841 ttcttcaaga actatatgac cactttcgta attagaacca tcaacagtaa tttttatttg
    21901 ttcaacattc atatttttta tgtaagtacg agcaactgaa tcaagcatta aagtttctgc
    21961 atcactacct aagtttgact ttgtaaagtc agagctaaca tctaatatac cagtttttac
    22021 accatcaatg tcttttgtag taaatgaatt taattttgca gattctggaa caactttaag
    22081 tttttgaagt tcttcaaata aagtgttctc atcaatttta tttaaatcaa ctttattttc
    22141 aattaaatct gtcttatcta catcaaaaga gtaaatagta gctgattgag ttttagtttc
    22201 ttcttttgga gtaggtttag ctttgttatt aggaacctct ttttcagtat tgttttcttc
    22261 ttttggttgt tcaacatttg tattgctctt agtttctttt ttagatgtat cacttccatt
    22321 ttgagcatta gtacaaccaa ctgcgaatat agatatagat aatatactta acactaacat
    22381 tattttctta tttttcataa aaatttctcc tctcttaaaa actattctta tatataatta
    22441 tagtcgttat gagcaaatat atgaatacaa aatagtaaca aatttaaaaa atgatttatt
    22501 taaattaata aaactcaaaa taaactaaat tttagtcatt tttatgggaa atcatttaat
    22561 gtacagtgaa aatattgtaa aataaaagta gtgtttttaa aataaatata agttataatt
    22621 atattataaa aaaatgtctt caaaaggtgg gaatagtatg gaaattttga gcttaggaga
    22681 aaagattaaa aaattaagaa aagaaaaaaa catgacatta aaagaattag ctggagatag
    22741 gataacagca gcacaaataa gccatataga gagggataaa tctcatacta gttatgaact
    22801 gttggaatat ctaggcgata agctagatgt aagtgtagaa tacttattag agaccaagga
    22861 aatgcaatca aaaaagataa ctgataattt aatactacag agcgaaattt atataaaaga
    22921 aaatgaatta aacaaggctg aggatcaaat aaaagaagtt ttagatatat gtaagagata
    22981 cgatttaatg gagaactatg gcagatgtaa ttttttaatg gctaatatta atttgaaaaa
    23041 atctaattat aatgatgcta tagaaaactt tgaaaaggca ttgtacttct ttataaaaaa
    23101 tagtgatagt gaaaatatac ttaagtgtta tttaaacata gggaaaatat atatgcagga
    23161 agacttttat aaaggtgcta taagccactt taattttgca gaggaaattt tgtcagagaa
    23221 taaatttgag gatgttaaca tacacaagga actatatagt aagatggcat attgttacat
    23281 aaaactagat aatccaaata tgtcattgaa gtacatagaa aagataaatg aaatagataa
    23341 tagaaatgat agaaaagaag atgtcgatat tttggtgtta aaagcaaata atatgcttga
    23401 gattggaaaa tatgaagaat caagagatta ctttaaaaaa gcactaaaaa tacttgagag
    23461 tgaggataat aaaactggat tagcaaatgt gtatttaaca atttgtgatg tttacagaaa
    23521 aataggtgaa acagatagag tacttgaata ttcacaaaag gtatatgaca taaagaaaaa
    23581 tgatgaagat gaatacatga tgtcaggatt atttaagata atagaagcat atatagataa
    23641 agaagattat gatttagcta gaaaatattg taagatagcc ttagcttcat caataaaaaa
    23701 taaaaataaa ttcaatgaat ataaagcatt gagattctat gctaatatgt ataaaaatca
    23761 gaatgaagta actcttgcaa ttgaatatct gattaaatgc ataaaaatag tatcagattt
    23821 aggcaacaat agaatattgg ccaatttata tatagactta ggtcaattat attcaaatat
    23881 atcaaaagaa aaagagttag aatattatca aaaaggtgta tttatgtaca aaaatttaga
    23941 aataatgtag ggtaaataaa cctataaggg tatagaaaca atctataaca agcaagataa
    24001 tatctatgat aaaatataaa ttgtatgaat atttttaagg agatgaatca gacaatgagt
    24061 ataaaaatag atgcgagagg gcttgcttgt cctaaaccag ttataaatac aaaaagagaa
    24121 ttggataacc tagaagaagg tgttgtagtt acaatagttg ataatgaaac agctaaggag
    24181 aatatattaa aacttgctaa atctttaagt tgtgaagcaa atattgttga cgaaaaagag
    24241 aattttatat ctatagaaat tagaaaaggt aaaaatatac tagataaaca aaatgttgta
    24301 gataataaag aaagtgaatt agacaataca tgtatattta tatcttcaga taagatggga
    24361 cttggaaatg acgaattagg aaaagtgtta ataaaaggat ttatatatac attaacagaa
    24421 tctaaacctt atcctaagta tgtactttta gttaatggag gagtaaaatt aagtgctgaa
    24481 aatgaagaaa ccatagaaaa tctaaaaata cttgagaata tgggtgtaga agttttatct
    24541 tgtggtactt gtttagatta ttataattta aaagacaaat tacaagtagg tacagttaca
    24601 aatatgtata ctatagttga gacattaaag aatgcatcaa ataccatttc tatataatgt
    24661 atgtttgttt aataatataa aactaataat aaagattaac tcaaatagat agtcagaaag
    24721 gattaagtaa tgaatcaaat gtatatagtg tcatttaatt cgacacatca tgcgataaga
    24781 tcagaaaagt tatttggtga aaatagtcta aaagtaatgg ctcttccaac accaagagaa
    24841 ataacagcaa gttgtggaat atcaattaaa ttttcttttg aagatatgga aaagataaaa
    24901 actattttgg ttgaaaataa tgtagatata aaaggaattt attgtataag taaattagaa
    24961 aatggttcaa aagaagtaga gaagttagat taggaggcta attatttatg ccaatggatt
    25021 taaaaatagg tgatattgtt gaattaaaaa agcaacatgc atgtgggtgt aaagagtttg
    25081 aaatcataag aacgggaatg gatataaaga taaaatgtac taaatgttca aggctaataa
    25141 tgcttgatag agagacctta gaaaagagag taaaaaagct aataaaaaag tgataaaaat
    25201 ttaacactta aaaaatgtca aataccgtat attaatatcc aaaattttgg tataataaga
    25261 attgaagaaa acattttcat gggggtgctt taattatggc agttaaatat gcaaaaagaa
    25321 tgcaaggatt acaaggatca gaaatacgtg agcttttaaa acttactcaa caaccacaaa
    25381 taatatcttt tgctggtgga atgccggctc cagagttatt tccagttgag gaaatgaaaa
    25441 aagtatcagt tgcagtgctt gaagaaaatg gaagatcagc tatgcaatat actacaacag
    25501 aaggatatga accattaaga gaaaaaatag cagctagaat gaatgataaa aacaaaacta
    25561 atgtaaacaa agatgatata ttagttacaa gtggttctca acaaggtcta gattttgcag
    25621 gaaaagtatt tattgatgag ggtgatgtaa ttttatgtga aagtccatct tacataggag
    25681 ctataaacgc atttaaatct tatcaaccta aattcataga tgttccaaca gattctgatg
    25741 gaatgataat ggaagaatta gaaaagatac tagaaacaac tgatagaata aaaatgattt
    25801 acgtaatacc tgacttccaa aatccaacag gaagaacttg gccacttgaa agacgtaaaa
    25861 aattcatgga aatagtaaat aaatttgaaa taccagtaat agaagataat ccatatggag
    25921 atttaagatt tgaaggagag actttaccat ctttaaaatc aatggataca aaaggattag
    25981 taatattctt aggaactttc tctaaaatat tctgcccagg atacagatta ggatggactt
    26041 gtgcttctcc agaaatttta tctaaattta actttgcaaa acaaggtgca gacttacaag
    26101 catcaactat atctcaaatg gaagtaagca aatttatgga tatgtatgac ctagatgcac
    26161 atgttgacaa aataaaagca gtttatgtta aacgtagaga tgtaatgctt aagactatgg
    26221 aagaagagtt cccagaagga ttagtattta ctcatccaga aggtggatta tttacatggg
    26281 tagagcttcc aagtaattta aatgctaaag aattaatgcc aaaatgttta gagaagaatg
    26341 ttgcttatgt tgctggagga ggattcttcc ctaatggtgg tagagaaaat actttcagac
    26401 ttaactattc aaatatgcca gaagaaaaaa taatagaagg tataaaaaat atagcagctg
    26461 tattaaaaga agctatgggt gtagaagctt aattaaaaat aataacttat atataataca
    26521 agggatggtt gttgccatcc cttgtttgtt tgaaaagtat ataattcacc ttatactaaa
    26581 ctttgtccaa aatttaaatt gacagaaata agaataaatg ataaaatgtg taggtatgag
    26641 attttagtct catttctctg ctctaagagt gtagagccgt taagtccaaa gggaggtgaa
    26701 ttatagtgag aaattatgaa ttagtttatg tggtaaagcc aaattctgat gaagaagtaa
    26761 gagaggctat actaaacaaa gttaaggaag ttgttgcaac tgacggagaa atagttaaag
    26821 ttgatacttg gggaactaaa aaattagctt atccaatagc taagtttaca gaaggtttct
    26881 atgtattagt taacttcaaa tcagcagtag acgttcctaa agagatagac agaaacttaa
    26941 agataaatga aaacgtaata agacacatga tagttgttgc ttaataactg gttttattat
    27001 aaaaaatagg tggtgttttt atgaatcaag ttgtattagt gggaaggttg actagagacc
    27061 ctgaattaag atacatccca ggtacaggaa caccagtggc atcctttaca atagccattg
    27121 atagagacta tgtaaaaaaa gatggatcta aagaaactga ttttatacct gttgaagtaa
    27181 tggggaaatc tgctgaattt tgtgctaact acataactaa aggtagatta gtagcacttc
    27241 aaggatctat aagagtagac aattatcaga cacaatcagg tgaaaagaga acttttacaa
    27301 aagtaagtac tagatcagta caagctttgg atagtaaaaa taaatcggaa aattcatata
    27361 gagagagtgc tccagctttt gagccaagct ttgaacctca aggtttagac ccacaaggtt
    27421 ttcaagctat agatgatgac gatataccat tttaaaaagg agggaaaaaa gtcatgatga
    27481 ataagaaaag acgtaagaaa aaaagagttt gtcaattttg tgctgacaaa aacgctaaaa
    27541 tagattacaa aagtactcaa agattacaaa agtatataac tgaaagagga aagatattac
    27601 caagaagaat atctggaact tgtgctaagc atcaaagaga attaacagtt gctataaaaa
    27661 gagcgagaaa tatagcactt ttaccatata cattagacta aaaaaatgag ccttgtgctc
    27721 attttttatt atatgtattt atatatctaa atgaggcgag ctataaagct cgtttttttg
    27781 tttatatact taaatatatt attatttatg atataatatt atatattaag gagggttgta
    27841 aacgtgaaaa tagataaagg ttctgagata acgagaaaca taaagataat agaatggatg
    27901 aaaacagaaa tattaatgag tgtaagtgat ttgtttaatt tattgtttaa aggggtaaaa
    27961 ccattagatg aagcaattca agatacttta gcaaatataa taatgataac atatctctta
    28021 gcaaagagat taggaattag ttttagagat gtggattata aagtcaagga aaaaataaaa
    28081 attggtatag atgaggacca tagcgttgaa agatggtatg gagatttatc taatttaaaa
    28141 aaacatatgg ataataggga gtgatattaa attgaataat aaaattagac tatctaaggc
    28201 aatgtctata atagttttag ctataataat cgctttagta atagcctata ttccaatgct
    28261 aggaatattt agtatgttag cagctgttcc atatgtaatt ataggtgcta ttactgatag
    28321 aaagtattct cttatatcta ttttggttac attttgtgta ctgattctat ttgcagacat
    28381 ttcttatgca ttaaatatat gtataatgta ttctttacca ggtatagtaa taggaaagat
    28441 gctaaagaga agctttgaac aagaggacag taataaattc gaaccgatat atggtggaac
    28501 cataatattt gtactttcaa tgcttgtata tttttttgta ttaaagacat ttttaaatgt
    28561 gaatctatta gatgaagttt ctaagatgat atcagaaatt gtaaatattc aaaaaaatag
    28621 tttttctgca actgaactta aagtgtttga taaaatgaag ccagatgaga ttgtaagtta
    28681 ttttacaaat atgctaccaa tgatgttatt tttacaaggg ttgttatcag cgtttgtcac
    28741 atattatttg agtgtatttt ttataaaaag gattacaaag atgaatataa gatttcctaa
    28801 atttgctgat ttttatttac caggaaatgc tatactcaca actttcttac tttatttatt
    28861 agtcttattt attcaaatta ttggttcaaa attatataca gaattaataa tgactaattt
    28921 acagctagtt tttaatttaa tgtttgtaat tcaaggtata gcagtttgca tttatttctt
    28981 aaagaaatgg ataagacagg gaccaaataa aattatgttt ttttcaggga taattttatg
    29041 tttatttgga tttatgggaa tatcttttgt tggtatggta gatagtataa ttgactttag
    29101 aaaggtaaga agctataaat ccacttagga ggatagaaat gagtaacaaa caaaccttta
    29161 aattaaatat gccagaaata aatttatata taattgttat tggtatttct agtataattt
    29221 tgctttatta caacttgtat gtagggtgtt tatttttctg tatttttgta tatatggttt
    29281 ttcacaattg gagaactact aatattagac gtcatgaatg gacagagtat attcaaaatt
    29341 tatctttaga tatagatgaa acaactaaaa aagccataat aaatcttcca ataccattat
    29401 gtattttaga atttgatgga aatatatcct ggtataatgg aaagttttat gatatgattg
    29461 gtcaaaaaga tttacttgat aaaaatatag aggatatagt taaaaatctt aatttaagaa
    29521 aagtattaaa tgaaaataaa gagatgtaca cagaaataaa ctataaagaa aaagaatata
    29581 caataattta taatgttata aaaaatgatc aagaaaaaaa tcctaaatat ttgatgatat
    29641 tatactggat agacaagact gaatatttaa aagtaaaaca aaattatgat gatgaaaaaa
    29701 atgcaatgat gctaatacaa gtagatggat atgatgaagt attaaaaagt gcagcagaag
    29761 ataagagagc tttaattaat gttgaagtag agaaaatact ttctgcttta gaattaaatt
    29821 caaatggtgc attgagaaga acttcaaaag acaaattctt cttggtaatg cataaaaaag
    29881 agctaaaaaa attggaagct gagaagtttt ctattttaga tacaataaga catattgatt
    29941 atggaaataa tcttcctgtt actataagta taggaattgg tatagatgga gatacattaa
    30001 atgagaacct aaaacttgct acgggtgctc tcgatttagc acttggtaga ggtggtgatc
    30061 aggctgttgt aaaaacaaaa gataaatttg tgttttatgg aggaaaatct aaagctgtag
    30121 aaaagaaaac caaagtaaaa tccagattaa taggtcatgc tttaagagag gttatacaac
    30181 aaagtgacca agtttatata atggggcata aatatcctga tatggatgct atgggagcag
    30241 cagttggtgt ttatgatata tgtaaatcct gtaacaaaac tgcaaacata gttttacaat
    30301 ctgtaaatga atctatagaa atatttataa ataaaataaa tgaaaataat tactataaaa
    30361 agctgtttat tggaaaagag gaagctattg acaactgtac taaaaataca ttagttgtag
    30421 tagttgatac tcatagacca aattatactg aatgtgaaga actgttaaag ctatcagaga
    30481 aggtagtagt tatagaccat catagaagag gtgtggagtt tataaatgat gcagtgcttt
    30541 tattccatga gatatatgta tcctcaacgt gtgaaatggt tacagagtta gttcagtata
    30601 tggatgaaga tgtaacaata aataaactta cagctgaagg gcttttagca ggtataagtc
    30661 tagatactaa aaactttgca ttcaaaacag gtgttaggac ttttgaagct gcttcttacc
    30721 ttagaaaagt tggagcagat actatagagg taaagaaatt cttcaattct gatgtaaaag
    30781 atttcataat aaaagctgaa ataattcaaa gtactaaaat aattaataat agaatatgtt
    30841 tagcatattc aagtactgag atagatagta taaatgttat aatagctcaa actgctgatg
    30901 agttattaaa tatcaaagaa gtagaagctt cctttgtatt aggtgaaaaa gatgatacta
    30961 tatttataag tgcgaggtct ttagggcaaa taaatgtaca tgtacttatg gaaaaattag
    31021 gaggcggggg acacatagat atagctggag cacagttaaa aaatgtttct ctaaaagaag
    31081 cttataagat ggtaaataaa ataatagagg agtatttgga ggaggaagaa tagatgaaag
    31141 ttatattatt aaaagacgta aaaggaactg gtaaaaaggg agaaatgaaa gaagtaagtg
    31201 atggatatgc aagaaatttc ttatttccta aaaagatggc tgtgcaagca gatagtgtag
    31261 caataaaaga attaaatgaa aagaataaat ctaaagaaat aaaagcacaa aaagagtatg
    31321 aagaagcagt tttattaggt aagcaaatgg aagaaattaa tatagaaata tattcaaaag
    31381 caggagaagg tggaagatta tttggttcaa taacttctaa agaaattgct gagcaattaa
    31441 agaaacaaaa agacatagac gtagataaaa gaaaaatatt attagatgaa ccaataagat
    31501 ctttaggttc aactttcgta gaaataaaaa tacatcaaaa agtaactact aaaataagag
    31561 tggatgtaaa agaaaaacag taataactag aggtgattaa gatggaagat atgacgagaa
    31621 ttcctcctca tagtgtagaa tcagaacaat caatattggg ttctatactt ctagataaag
    31681 atgctataat aacggttaca gaaactataa agcctgatga tttctataaa gaggctcaca
    31741 aaataatata tgaatgtatg ataactttaa gtaataaagg tgagcctata gacttgataa
    31801 cattaacaga agaactaaga aaacaagggc atcttaatga tattggagga atcagttata
    31861 ttacaagtct ttctactata gtaccgacga cttcaaatgt aaaatactat gctgatatag
    31921 taaaagaaaa gtctgtactt agaaagctta taaaagcttc taatgaaata ataaatctag
    31981 gatatagtgg agcaactaag attgaagatg ttttagaaca agctgaaaag agtatatttg
    32041 atatatcaca ggagaaaact agtgatgatt ttaaatctat aaacttagtt ttgatggatg
    32101 cctatgatat gatagaaaaa ttatatacta acaagagtga tgtcactggt ataactactg
    32161 gttttaaaga tttaaacaaa aaaataaatg gtcttcaaag gactgattta atattaattg
    32221 cagctagacc agcaatgggt aaaactgcct tttctttaaa cttggttcaa aatgcagcct
    32281 taaaaggtga tgcctctgta gctgtattta gcttagagat gtctaaggaa caattagtac
    32341 aacgtatgtt atcttcacaa tctagtgtag aattaaaaaa aataaagact ggaacactta
    32401 atgataatga ttggcctaga attatagatg ctatggcagt attatcagat gccaaaatac
    32461 acatagatga tactcctggt ataaaaatat cagaactaag gtcaaagtgt agaaagttaa
    32521 aaatagaaaa aggactagat ttagtgctta ttgattatct tcaacttatg gaaggtgaag
    32581 gtaataatga aagcagacaa caagagatat ctaagatatc aaggtcacta aaaatattag
    32641 caaaagaatt aaactgtcct gtagttgcac tatctcagtt atcacgtgca ccagaacaaa
    32701 gagcagacca tagacctatg ttatctgatt taagagagtc tggagcaata gaacaggatg
    32761 cagatatagt tatgttctta tacagggacg aatattatca tgctgattct gagagtaaaa
    32821 atataggaga agtaataata gcaaaaaata gacacggtga aactggttct gtggaacttg
    32881 tttggcttgg tgaagttcaa agatttgggg ataagttgag agacctataa tctaacgaat
    32941 atccacagaa cgtcctttct ttctacagga aaaatgtgga tacatgtcac taaatatgtc
    33001 caaacattga aatttagcta aaaaactatc cacactatcc acagatagcc tgttgataag
    33061 tgtgaataag tagataaagc aagcttttgg cttgctttat ctgtgtataa atattaatta
    33121 ttaggaggaa taatatggaa aaaataagct taaaatatat atatcccaat ataattaaag
    33181 tacttgatga aataaactta tttagagtaa ttgacaataa tttaagagag tctattgttg
    33241 tttatgctaa taatgtggat aatcagtatc atataaacat gactaataca aactttggaa
    33301 acataattaa catatgtaaa ttagaaaaac ttttagatgt ggataaattt atggaaaaag
    33361 ttataaaata tgaaaaagaa ataatagaga aagaagagtt ttcaaaaata gaagaatata
    33421 tgttaaatat tggagaatac taaaaaagtt gaaaaaaata cgaatgtttg atagaataag
    33481 taagtaaagt gttcgagagt atataaaata tagaggtgaa ttacatgaaa acagttgcaa
    33541 ttgttggttc tcaatgggga gatgaaggta aaggtaaagt tatagattat cttgctactc
    33601 aagcagatgt agtagtaaga gggcaaggtg gaaataatgc aggacataca ttagttgtag
    33661 aaggtaaaaa atacgcatta catctaattc catctggagt tttaaatcca aatacagtga
    33721 atataatcgg aaatggaata gtgtttgacc ctaaaggatt tttagaagaa ttggaaatgt
    33781 ttaaaacgga taatataagt acagaaaata taaaaataag tgatagagca catgtcatat
    33841 tcccatatca taaagaacta gatgcattgt cagaagaagc cagaggcgat ttaaagatag
    33901 gtacaacaaa aaaaggtatt ggaccttgtt atatggataa aacagagaga tctggtatta
    33961 gaatatgtga cttaatggat aaagataaat ttgctataaa attaaaagct caaatagatg
    34021 ctaaaaataa aatagttaaa aatatatatg gaaaagaaga attatttgat tttgaaacta
    34081 tatataacga atatctagga tatgcagaac aaataagaaa atatgtggca gatacgtctg
    34141 ttattgtata tgatgcagta agagcaggta aaaaagtatt gtttgaagga gcacaaggga
    34201 ctttattaga tttagattta ggaacttatc catttgttac atcttctcat ccaacatctg
    34261 gtggatttgc aattggtgca ggtataggac caaatatgat aaaagacgtt gttggtatag
    34321 taaaagcata tactacaaga gttggagaag gaccgtttgt tacagagcaa ataaatgaaa
    34381 ctggagataa gataagagaa caaggacatg aatttggggt aactactggt agaccaagaa
    34441 gatgtgggtg gtttgatgca gttatagtta agtatgcagc tagagttaat ggattgacta
    34501 gtatttcatt tatgttactt gatgtattaa ctggatttga taagataaaa gtttgtacgt
    34561 cttataaaat gggcgataag ataataacgg atttcccagc ttcattagat gatttagcaa
    34621 aatgtgaacc tgtatatgaa gaattagatg gatggaatga agatataact caaatagata
    34681 actttgatga ccttccagaa aatgcaaaga aatatgtagc aaaaatagaa gaactagttg
    34741 gagtaagtgt agatatggtt tctgttggtc caaatagagc tcaaactata ataagaagaa
    34801 atatatttgc ttaatatttt atcaattaaa taatttaaaa tagttgaaga agagtatctc
    34861 aagataatgt ttatagttat tagagatgct cttttatatt tgacttacat tgatttttta
    34921 ttaagttaaa gctacttata catattctag ctgaaattaa agttgaattt atgtagatac
    34981 atataggttt tttaaaaaga attttgttga aaataaatgg ctaaagatga ttttaattat
    35041 attctaaaaa aagtaaaaaa aactattgac taaaaattgt atagacgcta taatataact
    35101 tgtgagtgaa agaaacgacg aaaaaagtcg aaaaaatgtg gtctattagc tcagtcggta
    35161 gagcacctga cttttaatca gggtgtcccg cgttcgagtc gcggatagat caccaaaata
    35221 tggctcgttg gtcaagaggt taagacaccg ccctttcacg gcggtaacag gggttcaatt
    35281 cccctacgag tcaccagtat gggactatag ctcagctggg agagcacctg ccttacaagc
    35341 agggggtcac aggttcgagc cctgttagtc ccaccaaatt ttgaattaga gataaatact
    35401 agattatatg ctggtgtggc tcaacggtag agcagctgac ttgtaatcag caggttgtag
    35461 gttcgattcc tatcaccagc tccacaaagt acataaattc aaattaatat aattttctat
    35521 gttacaggat acttttaagt atcttttttt aatttcattt ttattgtatt aaaaatgaaa
    35581 ttaaacatac aacaaaattt tgaattactt gcaatgtgat agttttaaat ataaaattta
    35641 gaatcattct agcatagtat tatgtaatat agcaaattta atttatagta tcaataaata
    35701 tagactacta agaatgtaaa tgatttgtta gattaagata cattagatat gtaaatagaa
    35761 aaataaaaaa tatgttttaa tttatgtggc ataagtttta aacagagaag caagtttgct
    35821 cttacctctc tatttaaaaa taaaaaaatg ttttttaagg gctattttat ttaaatcttt
    35881 cccatctcaa atcatctcca gtaaattcta aaatcctaaa cttatctaag attctagaga
    35941 gagttctttg agtgtaagta tttcctattt gagatggtgt taagttggtt gaaataatga
    36001 tttttttacc agcaacaagt cttgtattta caatattgaa gatttcccca ctagtaaaag
    36061 aattattaag ttcagttcca aggtcatcta ttattaataa atcacaatca aacaagtttt
    36121 tataattatc ttcacttatt tggttatttg tgtctcttct aaatttataa tcttcaagaa
    36181 tatctaaaat tctaaaagaa gtttgataga ttacaacatt tcctttatct aacaattcct
    36241 ttgcaataca attgcacata taggtctttc ctagtccagt agaaccataa aacagtagat
    36301 tttcactatt atccttctta aaatcatgaa caaaattttc gcagatactt aaattattta
    36361 gcatattttc tctaggagat atttccccat cagagccttt cttaggagaa aatatattca
    36421 agttaaaatt cgaaaaattt tcctgactta aaatcctatc taaattagac attttataag
    36481 cttcatttac aatttcttgt ttgaggcaag aacatttttc tccattaggt aaaaatcctt
    36541 tatctttaca agaaatacat tgaaacttta tttccaagta atcaagaggt atgttacttt
    36601 cagataaaag tttttctttc tttactttta aagactctat attctcttta gtcttcttta
    36661 caatttcatc tttagatttg ggatttaaaa gtactatctt agctaaactg agacctattt
    36721 tagaaatttc atcatctata gattttatct cagggattct attatatacc tcattttttc
    36781 tatgttctaa taagagttca ttatcatctc ttctcttagc atatttagca agtatttttc
    36841 ttattttatc ctcattcatt taatcaccta cttaaacttt tctttttgac tttttttaat
    36901 aatctcatcg agctcatcag aggtatactg agtgaatgtt tcgttgaaat tatgaaactt
    36961 agtttttttg taagtattgt tgttttgagt agtattaatc tgcttcttat taatattctc
    37021 cttaactata cgttcttcct ctttttgctt taaatcattt aaatttttta tatttttttc
    37081 attccaattt ttaattatac catttatata agatatactt gggtttgaaa tattcttaga
    37141 cttagaacat gcatttatta tcaaatctat atccatatta aatttatcaa accaggtgtc
    37201 cataagttct ttttctgatt ttgatggttg tcttgaaaat cccaattcat tgaaaatcgt
    37261 tttatataaa atatatcgtt cacttcttac caaaaagctt tcttcaacat cttttggcgt
    37321 atataaattt gaatcatacc aatttctaat aatgccttca atgtatttaa ctggctttga
    37381 tgtaccagtt ttatctttga cgtattcata tgcgcaaagt atcatatcgg ggctcatatt
    37441 gtatttattc attatatcca taatactcat cttttcactt gggtcgagat atcgcccaac
    37501 tattttattt atagaattaa acatttttct aatacttgga ttttcagaag tagatacaac
    37561 cttatctgta ttagatttta tagatgaatt attgcttaat atattctcaa tatagaaatt
    37621 ttttaaatct aaaaattcta ttgaataatt aaaattatca tattctccat tgtcatgtat
    37681 ttttataatc ttcttttctt cccagaattt ccaagcagat aacacatcag acaatggtat
    37741 atttaggttt ttggctattg aattattgtc gaacttagga ttagaagtaa tatcacatgc
    37801 ttgtctatat cctaaaagat aaacttttac gtataacccg tccgccattg gcataaatat
    37861 atctataaag atattgggaa tagtgatttc acctaaatct atttcattgc tttctttaaa
    37921 aaacatttta tcacctcaga ttaaattata tcatttttta tattatattt gtctgtaaac
    37981 aaataaaagg tcacaagatt agcatgtaaa tattttctaa aagtattcat tttgaaaagg
    38041 aggagatatt ttggttataa aattcaacca aaaagtaaga agaacattat cagtagcgat
    38101 tctatttatg gtattaataa taggttgctt tatagaaaag gatgacagtt tagaaacata
    38161 caaagaaaaa actaattcaa aaataaatca gtataatatg gatgttatat ttgatgatga
    38221 gactaagagg ttaatgtgca atcagaatgt tgactacata aataatacaa aatcaaacat
    38281 agataaaata tatttccata tatatccgaa tgctttttcc aagaaagatt ttgctccttt
    38341 tgaaaaaagt gaaatgacaa gggcttatcc taatggattt aacgaaggat atatagatat
    38401 aaagaatata ttaaataata atagtaaaat ggagtataaa attaaaggag acaaaaatga
    38461 catattagaa atagaattag ggaaagagtt aaaaccaaat gaaagaatgt cattagatat
    38521 aaaatataac gtaaaaatac caaattcaat tggaagattt ggatatggag aaaatactat
    38581 aaatgttact aattggtttc ctatagcttg tgtaaatgat gaaaggggat ggaatttaaa
    38641 gagttatgaa actattggcg acccatttta tagtgagact agcgattttc atgtgaaact
    38701 attgattcca aataaatata aaattgctca tacaggaaaa cttattgatg ataaacatga
    38761 taataagaaa atgctatatg aaatagaagc tgaaaacgtt agagattttg catttatact
    38821 aagtagtaaa tttgatgtaa acaaagtaga ttcaaaggga gtagctatca atacatataa
    38881 tttaaataaa aagctttcta aaaaagcaac tgaagtggca aaagattcaa taaatatatt
    38941 tagtgaatta tttggaaagt atccttacaa agaattttct gttgtagcaa gtgacttttt
    39001 tatagggggg atggaatacc ctatgcttgt tatgatagac caaagtttat ataatgaaaa
    39061 aaatgaattt ttactagaat atgttattgc ccatgagaca gcacatcaat ggtggtattc
    39121 tgctgttgga aatgatgaaa taagtgaacc ctggttagat gaagcactta ctgaatattc
    39181 tacaatagtt tattttgaac aaaagtatgg taaagatatg gcaaacaagc ttataaaaac
    39241 tatggagata cagacaaaaa gttatttgag tgaaaatata tttaagccag caaatgagta
    39301 taaaaattct actgaataca gcttgaatgt ttatacaaaa ggcgcaatag catttaatga
    39361 aataagaaaa gaagttggag ataaagtatt ctttgagact ttaaatgaat actataataa
    39421 atataagcat aaaaatgcta atggaagagc ttttgtggaa ctatggaata gtaaaggtgt
    39481 agatataaat aagattataa gtgaatgtaa gtaaatagtg aatgtaagta aattttgtgc
    39541 agagtattaa attttattta atactctgtt tttttggtat aatataattt ataaggcaga
    39601 aaaagaggtg aaggaatgct aaagtattgt tcagtaggga gtggtagtag tgggaattgc
    39661 cactatatag ggtataaaaa tactaatata ctagtagatg caggattaag tggcaagaga
    39721 ataactacag gtcttaaaga cattggtgta gatgcagata aattaaaagg aatatttata
    39781 acacatgagc atgttgacca tataaaagga gcaggtatat tatccagaaa gttcgatata
    39841 ccagtatttg ctaatataaa aacatggtgt tctatgaagg acaaattagg tgacatcaaa
    39901 gataaaaata tgaaagtatt tgaaaatgat aaaacctact cattaggtga tatcatagtg
    39961 agaccttttt caatagaaca tgatgcgtct gatccagttg gatataattt ttatacagag
    40021 agtgatgaaa aaatgtctat agctactgat ataggatgta taactcaaaa tataaaaaaa
    40081 catctatata aatctaaatt agtagtattg gaatcaaatt atgaccctaa tatgcttatg
    40141 atgggttcat atacatatgc tttaaagaaa agagtaatgt caagtactgg tcatttatcc
    40201 aatgaagatg cagctaattt ctgcgttgaa cttattaatg aaggtacaga atctatatta
    40261 ttagcacatt taagtaaaga aaataatttt cctgagttag catatgagac ttcaaaagga
    40321 gtattagcat caaatgatat agtagttgga caagatgtaa aactagatgt actatcacga
    40381 aatgatgttt ctaatgttta tgagatgaag tagaggtgaa taactttgct aaagagaaat
    40441 tttgaaaatg ttgaggataa taaaatttta tactctataa agcagggtat gattttagca
    40501 atacctgcta ttatgactgg ttcaacggct cttgtaattt tgaatcttcc aataaaggct
    40561 tatcaagaat atttatcagg cttgtttaat ggagaggtta caaatattct taactttata
    40621 aatgattcta ctttaggaat tatttcatta ataatattat taacaataag ctatagttat
    40681 ggaaaaatat atgggagcaa gtatacagta cttgttccta tagttgcaat gtgctctttt
    40741 ttagtatttt cacatggtaa tgagcttaat tcttatatag aaatatttaa gacaaagtgg
    40801 ttatttacat ctataattgt ttctatgaca tcttctgtat tgtttgtaaa acttacagaa
    40861 tcttttgtta caagtgtaaa gtttcataca gaaggggctg atgcagactt taatatggta
    40921 gtttcggcta tagttccttt cataatagta gtatttttgt tttctatttt tagagtgata
    40981 atgataagtc taattggcag ttcaaacttt caagatatat tttataattt attttcaaat
    41041 gtttttaata aaatgggaac aaaccttatg agtgctctgt tgtttatttt tttaatgcac
    41101 tttatgtggt tttttgggat tcatggtagt aatgtattag ataccgtcgc aaaaaattta
    41161 tttgaaaata gtatggcaat taacataaat ttagttaata ataatcaatt accaacagaa
    41221 atttttacta agaccttttt tgatacaatg gttttattgg gaggatgtgg ctctttacta
    41281 tgtttagtaa ttgcaatttt tcttagtgaa aaacgtataa atgtaagaaa gctagctaaa
    41341 attgcttcaa taccagctct ttttaatatc aatgagatgc tagttttcgg gataccaatt
    41401 gtttttaact atattatgtt tgtacctttt gtaattactc ctataatctt aaccattaca
    41461 agttatgtgg ctatgtctac aggtattgta ccatgtacag tttcagctgt agaatggacc
    41521 tcacctatat ttttaagtgg atatatgtct acaggttcaa taagaggtag tttgttgcaa
    41581 gtatttaatt tatgtgttgg tgttatgatt tatattccgt ttataaaaat gtcgcaaaat
    41641 agatatacat atatgtttaa gaataatata gaaaatttaa caaacttatt aaaaaggtat
    41701 gaattaacag gtgaacagcc taatcttcta aaccataatg gtaaaatagg ttcaatttca
    41761 aaaatgttgg catcagactt gaagtttgct atgaaaagag gagaaataga acttttttat
    41821 caaccacaag tcaattataa tggaaatgtt gtgggagcag agggattact tagatggaaa
    41881 cattcaatag gagggttcat ttatccacca ctagttataa ttttggctaa agaagaaggg
    41941 ttcttagatg agcttggaga gtatattatt aataaagcat gtattgattt aaagaagagt
    42001 gaaaagctat tgagaaatcc tgttaagttc tctgtaaata tatctccaga ccaacttgat
    42061 gaccctaagt taccggagca aataaaaaat atcattagta gtaatgatat aaatcctaat
    42121 atgttaggta tagaaataac agagcaaatt gcattatcag gttcacaaat aattattgaa
    42181 cgtatcaatg cgatacataa cttgggaata aagttaatta tggatgattt tgggatgggg
    42241 catagctctc ttatatatct tcaaaataat aactttgata tagttaaatt ggatggttca
    42301 ttagttaaag agatacttac aaataaaaga agtagtgaaa ttataatgac tattgtaaat
    42361 ctttcaaaaa atttagattt tgatatagtt gttgagtatg ttgaaaatat agaccaaagg
    42421 gataagttat atggtttagg atgtgaaata tatcaaggat attattatag caaagcaata
    42481 ccttttgaag agtttgtaga atatgcaaat caagaaaaag agatataatt ttataagagc
    42541 ttgtaaattc tagtaatgca tagatttgta ataaaattaa gtttaggaga aatatatgaa
    42601 tataagtata gtagtagtag gtaaaattaa agaaaaatat ttaaaattag gtattgatga
    42661 gttcaaaaaa aggctttcta agtattgtaa gttagaaatt atagagttgg atgatgaaaa
    42721 agctccagag aatttgagtg ttaaggaaat ggaaattata aaagacaaag aaggaaagaa
    42781 aattttaggg aagataaagc ataatagtta tgttatagtc ttggctattg atggtaaggg
    42841 tttatcctct gaagaacttg ctaagactat gagtgattta gcagttagag gtaatagtag
    42901 tctctgtttt ataataggag gttctttagg gctttctgat gaggttttag gaagggctga
    42961 ttataggtta tccttttcta agatgacttt tcctcatcag atgatgagat tgatattgtt
    43021 agaacagatt tatagggctt ataggataaa taatggagaa ccgtatcata aataaatgat
    43081 acggtttttg ctaatttgac acttaataat attaaaataa caaaaaagta ttgaaagtat
    43141 attgcattta aggtagaatt aaataaaata ttataacatt ataatgttta tatagttgtt
    43201 aataaaaatt tggaggaaaa tatatgataa aaataatgtt ggcctgttca gcaggaatgt
    43261 ctacaagttt attagttact aaaatggaaa gtgcagctaa agaaaatggt atagagtcgc
    43321 aaatttgggc aataccagaa tcaactatac aaaatgaaat agaaaaatgt gatgtattat
    43381 tactaggacc acaagttaga tatgtattac ctaaggctca agaaattgct aagccatata
    43441 atataccagt agaggttata aatatgatgc actatggaac agttaatgga gaagctgttt
    43501 taaatagagc gatagaactt aacaacacta agtaatataa attataatgt atttttaata
    43561 tataaattgg gagaatcaaa ggatggatga aaagattata gaaatatctt ttaatataat
    43621 aggttatgca ggtgaaggta aagggttagc ttttgaagca ataaaagagg ctaagaatgg
    43681 aaatatagaa aaagcaaaag aattattaaa agagtctaag gaagttataa ataaagcaca
    43741 tagatatcaa acagaattaa tacaaaacga agctagtgga aacaggactg aaataagtgt
    43801 catacttata catgcacagg accacctaat gaatggtatg aattttcaac agttagctga
    43861 agaaatcgtt gatttacatt tgaaattaca agataaataa aaattaaaga gaataaatta
    43921 agagagttct taaaagatac attatgtgta gcacactaat tgttagatta ttgattcaat
    43981 atttagtgag taatacatta ggattatttt gaggactctt tttatattta gtttaaagtt
    44041 aataaatatt tattattaat aatataagat tatatgttat aatattataa catataattt
    44101 aagaggtgaa tcatgttaag aaatcaacca ttatatatac aagttgtaga tatattaaag
    44161 caaaaaatat taaataacga atataaaatt gatgaaaata ttccaacaga aagagaatta
    44221 gagcaagaat ttggagtcag taaaattact attagaaaag ctattgagat attagaatca
    44281 gaagggtatg tagagaaaaa aagtggaaaa gggacaaaag taataagtaa tagtcttttt
    44341 aataagctat caaaagcaga gtctttttca agtatacttt tatctaaagg tcataagtta
    44401 tctaaaaaga ctatttcaat tgaaaaaata ataaatgata caaattcaca agtatttagt
    44461 atatttggag aaaaatgtag caagattact agaatgtatt acttagatag taagccatat
    44521 atatttttta ctcattatat tccttttgat gaaaatctaa ctgttgaaag ttttaataat
    44581 gaaagttctt tatatatgta cttatatagg agaaatataa atataactag atttgaagat
    44641 gaatttttta tagaagaatc agaactagag gtatcaaagg ctctaaatct agctaaagac
    44701 atattattag gaagaaaaag aaagacctat gatgagaatg ataatatagt tgagctatca
    44761 tttgcaagat ataatacaga agtacaaaac tacgtaatta aatacgaaat ataataagaa
    44821 tcctgagttt attgaaatga taatatgctc aggatttttg tttagttgta aaaacgaaaa
    44881 atattataac attataatgt ttacaaccaa acattataat gttataatat ttttaataaa
    44941 taagtgatat tatcaattac ataaaaatta gtttatgtta tgaaatcgtt attgcattat
    45001 aaataaagga taatatcaat aatacagggg gataactatg gaaaaattcg caagtttttt
    45061 agagcgcaag attatgcctg tagcagcgaa gataagtaat cagagacata tgagagctgt
    45121 aagagcaggt ataatagcaa cattaccact aacaatagtg gggtcatttt tcacaatatt
    45181 attaaacata cctattgatg cttatatgga ttttatagct ccatttaaag caacattaga
    45241 tataccattt agatatactg taggattttt atctctatat gctacttttg ggatagcatc
    45301 atcacttgct aaaagctacg atttagatac aacaggtgtt ggtatgcttg cagttatggc
    45361 atttttagtt tctacaatag ttccaactca agtagttgaa ccagtaggtg gagttataga
    45421 agctggtaga tggatggata tggcaaaact taattcgcca tcattatttg gagctatcgt
    45481 aacatcatta attacagttg aaatatatcg ttttatgaag gaaaagaata ttacaattaa
    45541 gatgccagca ggagttccag tagaggtttc aaattcattt actgcattat taccgacagc
    45601 agtaatttta atgttattct gggtaataag acatatttta ggatttgata taagtgttgc
    45661 actaagcgct atgttatcac ctttaaaagg attcttatca ggaaatagtt taggtggtgg
    45721 gctactgaca gtattcctaa taactttatt ctgggtactt gggatacatg gtccagctat
    45781 aatggggcca gtaataagac ctatatggga catgtctata gcagaaaata taactgcatt
    45841 cacagaagga gtatcagctc atgcaatgcc aaacatattt acagaacaat ttttacagtg
    45901 gtatgtatgg atagggggag caggggctac tcttgcatta gttgtaatgt ttatgttttc
    45961 taagtcacaa tatctaaaaa gtttaggtag attaggattt ttaccagggt tattcaacat
    46021 aaatgaacct ataatatttg gagcaccaat agttatgaat ccaatactag ggataccatt
    46081 tataattgga cctataataa tgacaattct atcatatgtt cttactatta caggggttat
    46141 acctatgatg gtagcaagat taccatttgc aatgccagca ccaatagcag cagtaatgag
    46201 tactaactgg agtatactag caggtatctt agtattaatc aactttgtga tatcatttgc
    46261 tgtttattat ccattcttca aagtttttga aaaacaacaa ttacaaagag aacaagaaga
    46321 aatgagtgaa aatttataaa aatacataga tatagaggtt ttgtactata taaaatatag
    46381 aaaaagctta taagttatta agggtgaagg taggaggaat gacctttgcc tttaatttta
    46441 tatattttaa ataatgtaat ttgaggagga gaaagatgga aagaagatta ggaatatcag
    46501 tatatccaga gcatagtaac atggaagatg ataaggcata tataaaaatg gtttcaaaat
    46561 acggatttaa aagaatattt atgtgcatgc tttcaattac aagacctaag gaagaagtta
    46621 aaaaacattt taaagagatt ataacctatg caagagaatt aggatttgaa gtaatccttg
    46681 atatagctcc aaacatattt gatatattac aaatatctta tgatgattta acattcttta
    46741 gtgagttaaa tgctagtgga ataaggcttg actatggata tgatggaagt aaagaagcta
    46801 tgatttcttt caatccatat gatttaaaag tggaactaaa tatgagtaat gatgtagcat
    46861 acttggataa tataattact tacaagccaa atgtaaataa tatatatggt tgtcacaatt
    46921 tttatcctca aaggggtaca ggcttgccat atgatttttt tgtaagatgc tcaaatagat
    46981 ttaaaaagta tggaataaga actgctgctt ttgtcaattc aaaatacgca acaatcggac
    47041 cttggaatat aaatgatgga ctgtgtacgc ttgaaatgca tagaaactta gatattgcta
    47101 cagcaactaa gcacttattc gcaacaggat tgattgatga tgtaattgta ggaaatgcat
    47161 atgcaagtga agatgaatta aaaagaatgt ctgaagtaaa tgaataccaa gttgtattta
    47221 atgtagtagc taaagaaaaa acttctgaag tagaaaagat aataatggta aatgagcaac
    47281 actttagaag aggagatata actaatcagg tagcaagatc tacagaagta agaaaaaaat
    47341 atgctaaaga aaatttccct aaacatgata ataagaacat ctttaaaaga ggagatgtag
    47401 tgattggtaa tgatgacttt ggaaagtata aaggggaatt acagttgata cttcaagatt
    47461 gtacagatga aagaaaaaat aaggtgggta gaataaagga agacgaaata atacttttag
    47521 actttattga accttggagt aaatttaaga ttattttaga gggagagtaa agtgaagata
    47581 gacatattaa taaaatgtgg aaaaacagta gataaaagta taattgatat agctatatta
    47641 gacgataaaa taatagaagt aaaaaatcat atagatgatt ctaagtatca ggcaaaacaa
    47701 attataaact tagatggaga gaaatttatt agtgcgggtt ggatagatat acatacacac
    47761 tgctacgaaa gactagacct ttataaggat tatccagatg aggttggaat aaaaagtgga
    47821 gttactactg tagtagatgc aggaacaaca ggagctttag atataggtga attttatgaa
    47881 gatactaaaa agtataagac caatgtatat gctttaataa atattgctaa gcagggaata
    47941 acatctcaag atgaactttc aaatatgatg aatattgatg aatatgaact taaaaatgca
    48001 gttagaaaat acaaagattt tgttgttgga atcaaagcta gaatgagtaa aagtgttgta
    48061 atttcaaatg atgttgaacc tttaaaagta gctaaaagaa taaaaaatga acttaatcta
    48121 cctatgatgg ttcactttgg gagttctcct ccaacgatag aggatatatt tgattatatg
    48181 gaaaaaggag atatattaac ccatatatat aatggaaagc caaatggaat acttagaggc
    48241 aatgaggtta aaaaggaaat catagaagct aaagaaagag gtataattct tgatgtaggt
    48301 catggaaccg aaagttttag tatggatatt gcaatgaaat ctaaggatgc aggaattttt
    48361 ccagacacta taagtacaga tatatatatt aaaaatagaa taaatggtcc agtgtataat
    48421 ctatctacaa caatggaaaa gtttatatat atgggctatt cacttgaaga tataatagat
    48481 aaagttacta aaaatgcagc agatgcaata agtttaaaaa ataaaggttt aataaaagaa
    48541 ggatatgatg ctgatttaac tatatttgat gtggttaatg aagaaaaaga acttcaagat
    48601 tctttgaata agtcagtaat cacaagtacg tctataaagc caagagcagt agtagtcaat
    48661 ggagaatatt taaatatagg caactctata gaagagaatg agtagaaggg agaaactatt
    48721 gtatgagtag tatatttgaa aaatactctg taagacgtgt tataaatgct tcaggcaaaa
    48781 tgactatact aggagtatca acagtagata aggaagttat tgaagcaata ggtgaagggt
    48841 gtaaaaattt ccttataata gaagaacttg taaataaaac aggtgaatat atagcaaatt
    48901 tattagggtg tgaaagtgca ctaatagttt ctagtgcatc tgctgggatt gcacagagtg
    48961 tgggagcaat gatagcaaaa gatgatatga gtctagttta caatgtaaat aatcctaaga
    49021 aacaagttaa aagggaaata ataattccaa aggggcataa tgttgattat ggagttccag
    49081 tagaagtaat gataagactt ggaggaggag aagttgttga ggccggctat gctaatatgt
    49141 gtaacaagga acatataata agagagataa atgaaaatac agcagcaatt atgtatatta
    49201 aatctcatca ttgtgttcaa aagagtatgc taagtgtatt agatgcagta caagttgcta
    49261 aagaatataa tttacctttg atagtagatg cagcagcaga agaagattta aatgtatatt
    49321 atgaattggg agcagaccta gtgatataca gtggtgctaa ggctatagaa ggtcctagct
    49381 cggggttagt aataggtaag aaaaaataca tagataatgt aaagcttcaa tcaaagggta
    49441 taggaagagc tatgaaaata ggaaaagaaa atattgtagg tctaacatgt gcaattgaga
    49501 gatatataag taatgataaa gtaactttaa aagaaatgga ggacaagcta aatccattta
    49561 tagacaagat aaatactata aagggagtga gtgctagtat aactagagat tcagcaggta
    49621 gagaaatact tcgtggagaa attaacttta atgaagatat aataaataag tcaacacaac
    49681 aaattataaa tgaacttaga agtggagaga tagctatata cactagagac tataaggcta
    49741 atgaaggcaa gattgagatt gatataagaa gtgtaactgg aagtgattta gataatattt
    49801 ataataggat aaaaaatata gtagaaagtt agagtgtaga gtaagtgaca ttataatgat
    49861 ttaataaatt gaattttacc aatggagggg ataaatatga attgtatacc acaatattat
    49921 aaaaatagag tttgtttaaa tgttcttgca ggaagcataa aaaatgctaa agaagtatat
    49981 gatgcctgtg atggacatgt actagtagga gtactatcaa agaattatag cactgtagaa
    50041 gcagctattg aagatatgaa aaaatactca aaggaaatag ataatgcaat atctgtgggt
    50101 cttggagctg gagacccaaa tcagtcaaat atggtatctc agatttctaa agaacttcag
    50161 ccacaacata taaatcaagt gtttacaggt gtcgctacat ctagagcatt gcttggtcaa
    50221 aatgatagta taataaatgg attagtttct ccaactggta aaccaggatt tgtaaaaata
    50281 tccacaggtc cattaagttc aaaagaaaaa gatggtatag ttcctataga aacagcaata
    50341 gctatgatga aagatatggg aggaagctca attaagtttt ttccaatggg agggcttaaa
    50401 acaaaggatg aatatataga agttgcaaaa gcttgtgcaa agcataattt ttatttagaa
    50461 ccaacagggg gtatagactt agataacttc aaagagatag ttcaaattgc acttgatgct
    50521 ggtgtagaaa aagttatacc tcatgtttat acatcaataa ttgataaaga aacaggtgat
    50581 acgaaagtgg aagatatagg agtactttat aaaataatga aagaattatt gggatagatt
    50641 tatgaatata ttagcttttg gtgaaataat gatgagactt tcagtacctg actacaagtt
    50701 cttaacacaa accaatgaac taaattatat aattacaggc acgggattaa atatattatc
    50761 tggacttaaa aactttggat acaataccta tatgttgaca aagttgccta ataataatgt
    50821 aggtaaagca tcaagtgcaa atataagaaa gcttggagta aaagatgact ttatcacata
    50881 tggagggaat catataggag tgtattttct tgaaaatggg tatggagaaa gaccttctga
    50941 agtgacttat ttgaataggt taaatagttc attttgtgag agtaaattac aagattatga
    51001 tattgataaa tgtttagaag aaatggatat agtccatatt tgtggtattg ctatgcaact
    51061 tacagattca gttaaaaaga ttgctttaga actagctaga aaatcttatg aaaaggggat
    51121 aaaagtattc tttgatttta attttagaca atcattaaat ccagaaagga cttatgatga
    51181 tttagtaaat gattataaga aaatactacc atattgtcat gggctgtttg ctagttatag
    51241 agatataaat aagatactta atatagatag tctaaatata aaatctagtt cagaattgat
    51301 taaaaaagag tatgatatag atattgtagc tggaactatg agaagatttg atgatatgaa
    51361 taatagattt ataaagggat atatatcaaa tgatgatggt tattttgaaa gtagggagta
    51421 taagttagaa atatatgaca gagttggtgc tggggatgga tatgtggcag gaattatata
    51481 ttcatatttg gaaaatatga agagtgaaga tatagttgag tttggggttg ctagttcggt
    51541 gttagctcat acaacttatg gggataattc tttagtaagc acaggtcatg tacttagact
    51601 gatgaacaat gaaaaaatag atatattgag ataatacatt tgtatataat aaaattttta
    51661 tcttttttag acacactttt gacttgctta tatttgttaa acagagttat ttttaacgat
    51721 tataaatatt aaattaaaag ttagataata aaatagtaga aaatagcatt aaccatataa
    51781 aaatggttaa tgctattttt atagttaata ttgattaatt ttatattata gaaatgaact
    51841 tttctaatgc tggatttgta ttttttttat tccatacaac cacaacttca attctatttt
    51901 ctacttcttt tataggtact attgcaatat cacgttctat cattgtatct gtaacacaaa
    51961 atgaaggtaa aatggatatt cccatgttag aagataccaa tgattcaata gtattaagat
    52021 tatttgaacg ttgtaccaca ttaggtacaa accccgcttc acgatatcta ttttgtatca
    52081 ttgacttttg tccatactca tcccctgttt cttttacaaa aataaattta tcatgttgta
    52141 attcaattaa atcgatagaa tcctcattgt aaagtggatg accaggaggt aaaagagcta
    52201 ccaaagaata tctttctaca gttttatatt caaaatcttt taatggttga ttttctggat
    52261 taataacaaa agcgaggtcc atagaattat tttttactaa gtttaaaagt tcggttattt
    52321 cattagtaca aatatccata gatatattag ggtatgtatt agaaaattct tttaagtatt
    52381 gttgaagttt gtttttttca tatcctatta aggttccaat gctcaaagtt ccaaaaaaac
    52441 catgtgcaaa ttgttgtgtt tttgcaatag catcattgat attttttatt atagtacgtg
    52501 cctcagataa aaaaactttt ccggctggtg ttagttcaac atgacgttta cttcttgtaa
    52561 atagagttac atttatttgt tcttctaaag cttttatttg ctgtgtaaca gcagtttgag
    52621 aaatataaaa tttttctgct gttttggtaa aacttagatt ttctgctaca gatataaaat
    52681 attctaattg tttagtattc aaataatcac ccctatttaa acttgattgt ttgtaaacct
    52741 tatttatcta taattaaagc ttattatagt ataagaaaat ttacttttaa agaatattag
    52801 aaaatatttg tataaaaata tatgttttaa gtatatcttt acattagatt ttataaattt
    52861 attaataaga ataacttata tattccaatg aaaacgatat tggatttaag taagtttgtc
    52921 tcataaaatg aaagtatatc aattgataat actttaaata agaagggaga aaaaatggaa
    52981 gataaatttt atgcaaaagg caacggaaat aacggatata ttaaaaatct tgaagtttgt
    53041 tcctttaata acttagatgg aacttgtgga atgtttcaaa tggctctgta caaaagagat
    53101 gaaaaatact atttatatgg atgctgtttt ggaggaaata aaaaaaatgg agtaatgatt
    53161 agcgatatta cagaccctta taatccacaa tttataaaac attttcaaat gttagaccct
    53221 aaagagtatc ctacaacaac aactccaaaa attcaaatag cagatgattt aatgatagta
    53281 gcaatgagtt gtggaagtgg accaggagca cttgttgacc aagctaaatt agcaaatatt
    53341 aagtgtgaag caggaattag aatatacagt ttaaaagaag accctttaaa tcctaagttt
    53401 ttaggatatt gggattgtgg cttaaagcat gtaatgggtg ttcatagatt catgtacaat
    53461 ggtggaagat atgtacattt atcaagtgat tgtgttggct ttgaaggtct gatttatagg
    53521 gtcatagata taataaatcc taataatcca gtggaaatag gtaaatggtg gagaccagac
    53581 caatatgcag atggatatcc aaatagaact tttgatgcag gagcacctca ttgcccagaa
    53641 tttatggata aaggatggct tcatggacct ccatttgtaa gagacggaaa agcatattgt
    53701 ggttatggag gagctggttt agttgtatta gatgttgaag atttaacaag accaagatgc
    53761 ttaggtgaat tgccatttat gcctgcattt tctagtagac ttgcaggtgc aagaactcat
    53821 acagcattac cattgccagg aagagattta gtcgttgttc aaaatgaggg agaaagattc
    53881 cagttcttta aaccagataa cattacagat gttcaagcta tgaataatat acatatggtt
    53941 gatgttagtg acccaacaaa accaacatta attgctcaat tcccatatcc tgaagttcca
    54001 aaagatttcc cttatcctaa ctttaatgtt gcaggattag gaaaagcagg gccatttggt
    54061 ccacataatc ttcatgaacc aatggataat aagccatggt tagagcaaag aggagataga
    54121 gtatattgct gttatttcca tgcagggcta agggtttatg atgtatcaga cccatattat
    54181 atcaaagagc tagcatattt tataccacca aatccaaata aaacaccaga agaatcttat
    54241 ttcccaggat tcccaggacc acgcttggca gtaacagaag atcttatcgt tgatgataga
    54301 ggctacatca tcatagatgc tttagatgat ggattctata tattaaaaat gaaagaagat
    54361 taaataaata ctaaagaggg agagttgaaa atgaaaatac taggtatttc ttttggaaca
    54421 aaaaatggaa ataatgacac aatgtgtaaa gttgcactta aaggtgccaa agaagcaggg
    54481 gctgaagtag aatttattca aatgtcatct attaacatta aacattgtac tggatgttgt
    54541 gcgtgcgtta aaactttatt atccgggaaa ggtagtatgt gtgtattaaa agatgatttt
    54601 gaatggttgc ttgacaaaat gaaagatgca gatggtatag tagtttcaga ccctattttt
    54661 gaagaagggg catcaggatt attccatact ataatggacc gttttggacc cagagcagat
    54721 agaggaaata acattatagg aacaaaagtt gcagaagcta taggaggaaa aattccagac
    54781 tcaagaatgc ttaatgataa agtaatatca tttatgggaa taggaggttc tgactggggt
    54841 acaagagttc aatgtgaaca tgctatgcta gctctcatac caatgtggaa agttatagat
    54901 aatgcttggt tcccatgggc aaaggaatta gtaatggatg atacaaggct tgttcaagtt
    54961 catcaaattg gtttaaatat agttgaagca gcaaaagatt ttgaaaaagc ttcatatcaa
    55021 ggagaagaag gtgtatgtcc acattgccac aatagattat tctaccttga gtcaggtact
    55081 aaaaaggcaa tttgtgctct atgtggtata gttggagagt tagatactat aagtggaaaa
    55141 actatattta gttttccaga agagcaatta ggtcatgctc atgatacttt accaggtaag
    55201 tttatacatg gtgatgatat taagaaaatg gaaggtcatt atgcagaagt tcgtaaaaca
    55261 caagaattta aagaaagaaa aacatcttat aattttatag agccattaac taaagaaaag
    55321 caagctggag tttcattagg ataataatta aaatactaag actctttttt aagggatgtt
    55381 agatgttata ggaggcttat tgtatgaatg aaaagccaag ttttaaacaa attgaaatta
    55441 ttttagcagt tatttttttg gtacttacat attacaagat tggattaact ccagcattta
    55501 ttgttatatt tataccatta tacttaagtt attttgcagt atctatgatt gtaaatcaaa
    55561 tagcaattaa gctattggag ataaggcaag ctagggaagc tactaattac aatataggaa
    55621 atggatagaa agaaaaaaga aatattaaca ctgtctatgg ggattgcttt actacctcct
    55681 ttatgggcag tattagcacc ttatatagga ataaaaactg gagctgtagc actaatctgt
    55741 gcaggattat atgtaactaa tggtaataaa caaaaagatg gattaaaaat aatgtttggt
    55801 ttttggtgtg gagatttatg ggcagtgtta gccattttaa ttatgggata tatgaatttc
    55861 aatcaaaacc tagaattatt tttaacactt agtatattag gtttttttgc agttgtaatt
    55921 gcatccttat ttgaaaaaat aattttccta ccatcatggc tttgtggttg ggctataggt
    55981 ctaactatta tgacaggtga aaatattatt aatttacaag atatttgtat tcaaattgct
    56041 gtagctatgg cagtaggagt atggtacgtg ggtgcaggtg tagatttgtt tacaatatat
    56101 attttgagta aagataagaa aaagggaaat tgaaagcatg tgaatatact ttgattatag
    56161 gagaatggct atgggttttg aatttaaaat aatgagaagt ttaatatatg taggacttgc
    56221 caaggaagaa tatagaccta agttaatgga ctggttatat cgtcaccata ttccagatag
    56281 tattagcact tttggaccat attgtactaa atatgccttt tatcaagcat atcctacacc
    56341 aaatgaaggt gagcgttttg gtgcacgtaa gatgcaacta acagaacatt attggcttgt
    56401 agatgaacat atgcctgaga tggcaaatag aattatgaca gaatatatgc ctatggatgt
    56461 tctacgttgg caagggtgta taccagatgt agaaaataaa agggttcatg aaaatgcaga
    56521 aagtggagat gcaggacgtg cagtaggtgg agataatgga tgcccaccat ttatatttgc
    56581 ttttgttcca ataaactggg aagaagactt tagaggaaaa ggacgtactg tacaagatgg
    56641 accaaactat cgttggcaat ttatgattaa gtatccagat ggtatctcta aagaagaagg
    56701 agaaaaatgg ttctatgatg aggtagtgcc atactttaca aactgttgct atgttaatcg
    56761 ttttgtcagt agtaaaataa tgattaatta tggagcaact gcttttgacc gtgtatcaga
    56821 actatggttt gaaggggaag aggaatggta taaagctgtg gttgaagaga caaagtcgtt
    56881 tattaaaaaa ccagaatggg cacaagaaga ggagttccca tatttaaaac cacaattcaa
    56941 tatcgcatca gtattcttag gtgatatagc aactatggat gcatactcac agtatcgtgg
    57001 atatatacca atgagataaa aattatagtt tgtgataagt agaggaagta cttactctta
    57061 ctgctagaag aagacttatt gtacacttaa aagaatgaaa tagataagtg tcaatttatt
    57121 atatgagaga aactttaact ttcttggttt aaaatattaa agtcataagg aacatttatt
    57181 attaaatact ttatttatat gaattaattg aagaaaaagg atattaaact acaagttaaa
    57241 aaataaattt aatatattag aattataaat aaggcacatt aattttattt taaattaatg
    57301 tgccttttat ttatggtaat ttatgcataa ttagtatcgc tttgattctt taaataaatt
    57361 ttttttggtt tctttacata aggaaccctt ttttatttat aaaatctata agtgtaatat
    57421 agtagctatt ttagataagt ttaatttttg tctcaatcta aaacaaaagt tatacttaaa
    57481 tgaaattgaa aacgataaaa ttctcaaata aaataagtat tgtggaggtg atgaaagaat
    57541 ccaatgataa tatgaagact aatcttaata agaaagtagg ggttaatggt agtggacaaa
    57601 gaaattgtat caattctcca gattttatta aatagcacat tgattacaac taatggatta
    57661 caagaggagt ctaattcatc aaagagacaa attacttata gaataaataa aatcaatgac
    57721 atgcttaaag taaaaaatgt accacttata tgtttaagag ctgataaaga tattatcgtt
    57781 agaaaagaaa caaaaaaagc aattaaagaa attttagaaa aaaactattc taagaatact
    57841 tattatttta gtaagacaga aagactttta tatatgtatt taatgctttt tattaacatg
    57901 gaggatattt caattaatca ttttataaat tctataaaag taagtaggtc tactgtaaat
    57961 cttgatttca aggatttgat accagagctt gaaaagaaga acattaaagt taagaacaat
    58021 cgtataaatg gatattacat agttggcaat gagatggaaa ttaggagagt gcttattaaa
    58081 aacattatag aaactttgtc taatgagaaa agttcaagag tatttgatat ttttattaaa
    58141 gaatataatt tagatagttt tgaagaggct aaaaaaatta tattaaagct aataaaaaaa
    58201 tatgaaatta cttttgttga agatagatta atagaattta tatatatatt tatctttcta
    58261 aaagcaagga tgtattcaac tagaatagtt actcaggaaa atgtagatat tcctaatatt
    58321 tcagttatga gttcaatgaa ggagtataaa tttgctaagg aattattaga agtatatggg
    58381 agtgaggata agataaagcc ttataatata atgtatatta gtgcttggat tttaggtata
    58441 tcggtaggaa attatgaaga agatacagaa gataaggatg taatttcaaa aattgtaaat
    58501 aaaatgatga caaagtttgg atatttaagc ggagtttact atataagcca agaaaaaata
    58561 tttaaacaat tatattctca ttttagacct gcctactata gacttttatt taaattacct
    58621 atctataatc cgatttgtga aaaagtaaaa gaagaatata aattagttta tagaattgta
    58681 agtaatgcaa tgaaagagtt ttgcggatta tttggagaag aaattcagga agaagagtta
    58741 gcatatctta caatgcactt tgcaacactt ttttcaaata aaaaagaatt tgatggtttt
    58801 agaaaaagaa tagcattaat agtttgttca aatggcgttg gtagttcagc tattttacat
    58861 gcagaactta caaacttatt tcctgactta cactttatac caatgtcgga atcagttgga
    58921 ttagaaaata taagtgaacc tgtagatatc gtatttacta ctaattataa cgcagagggt
    58981 ttaaaaacag atgtaccagt aattaaagta agccctgtta tgaccacaaa agaaaaatat
    59041 aagattacac gtgaagtgta tattcaatta ggtgatattt atttaagaca acctaaaata
    59101 gatgaggtta tggatataat aaaaaaatat gcaaatgtaa cctctgaaag tattctttca
    59161 agtgagctat tagcttattt tacacagata gagaatttta catctaagga aggagagggg
    59221 cctatgctaa gtgagattac taatgaaaca ctagtaaaaa taaaggtaaa agctaagaat
    59281 tgggaggaag ctattagaca gtcagcttct gtattagttg aaaataacaa agtaactgaa
    59341 gggtatgtag atgctatgat taactcagca aaagcatctg gaccatacat agtaattaca
    59401 aaacatgttg cacttccaca tgctagacca gaagctgggt caaaagagat tgcaataggc
    59461 attgctactt tagatagtcc tattgagttt gggaataacg aaaacgaccc tgtaaaatat
    59521 gtattttgtc taagtgcaat agacaataat agccatttac gtgctatgtc tgagctagtt
    59581 gaactattgg aagaagatga gttttttaag gttttagata gtgctaaaga ggctaaagaa
    59641 attattgatt ttataaaaac acacgaatta taaaggagga tttaattatg tataaaattt
    59701 taatcgcatg tagagcagga gttggctcaa gtttaatgtt aaaaataaag actcaacaaa
    59761 ttattaagga aaataacttt ccaattgaag tagaacatgg ttcattggat tcactaaatg
    59821 gattcaatgg agatgctgta gaaacattaa ttgatgttgc tgaagaatta aaaaataaaa
    59881 atttgaagtt tgatataatt gggattcaca atattgttga ccgtaatgaa ataaagacag
    59941 cactagaaaa attccttgct tcaaaagagg gataaattta atatgataag gaggaatatt
    60001 tatggagttt attattagtt tgctgagtac acctgctgtt ttgcttggcg tagttgcatg
    60061 tataggattg attcttcaaa agaaatcagc cgttgaagta tttacagggt cttcaaaaac
    60121 acttatcggt tttttgatat ttggtattgg tgcctctgct atgacaggag cacttcaaaa
    60181 tttcaataaa ctgttccaac atggctttgg aattactggt gttatagctt caccagaagc
    60241 agctacagca cttgcacaag gtacatatgg ttttgcagtt tcatgtacat taattcttgg
    60301 attcatatta aatttagtat ttgcacgtat tacaaagttt aagaatattt tctttacaac
    60361 aggtcatagt ttattctttt cttgtgtatt agttttaatc atgaaagcac atggatttga
    60421 taatacaatc actattttaa taggtggaac tatcttaggg tttatgtctg ctgcactacc
    60481 tcaattctgt caaccattta tgcgtgaatt aacaggtggc gacgaacaag ctattggtca
    60541 ctttaacatg attggttatg gtttatcagg ttatattggg cgtttatttt caaaacataa
    60601 agatgataca acagagacta tagaatttcc taagtggtta tcaatattca gagacttctt
    60661 aatgggtcta tccatagtta tgttaatctt attctatatt gcaacattaa aagctggtca
    60721 agcatttact caagaaattg ctggttcaac acattggttg gtttttccgc ttatagaggc
    60781 atttacattt gtggcaggta tgtcaatatt gatgtcaggt gttcgtatgt tcttagctga
    60841 aataactgca gcatttgtaa tcatttcaga aaagtatatt ccaggttcac gtcctgcgct
    60901 agatgttcca acagtatttc catatgcacc aaatgcagtt attattgggt ttatatcagc
    60961 atatgctgct ggtctattag caattgctat tatggcatca tttcctagta tatttcctgt
    61021 agtaattatt ccagctgctc acatatgttt cttctcagga ggtacagctg caatatttgg
    61081 caatacttca ggaggatgga gaggggctat tgctgggtca tttgttgttg ggttattact
    61141 tgcattttta ccagttatac tatatccagt ttatgggact cttggtatag aaggagcaac
    61201 tttccctaat attgattaca acgtagttgg ttcgatatta cataatattt tacaatttat
    61261 aaaacctatt taaactaaat tatataaaag tatagtcata gttactatga tttcaaggag
    61321 gatacatctt atggaaaata cattatctga atttttagat aatatatcta atgaattgtc
    61381 agaatttatt gcctcaatag atgagacatc tataaataaa gcatgcgaat taattcttga
    61441 agctgaaaaa aatcacggac gtgtacatgt gactggtatt ggtaaaccag gacatgtatc
    61501 aggatatatt tcatcattgc tttcatctac tggaacatca gcatatatat tgcatggtac
    61561 agaagcagta catgggtcaa gtggtcaagt agttgaagga gatgttgtta ttgcaatatc
    61621 aaatagtggt gaaactcaag agttaaaagc aactcttaag actctaaaag taaatggagc
    61681 aaaaataatt ggtgtaagtg gtaatgagag ttcattttta aaaaatattt ctgatatatt
    61741 cctgttcgca ggcgttaaac aggaaggaga ttgtcttaat aaagctcctc gtgcttcaat
    61801 tttagctgaa acaatagtat tacaatcatt aagtgttgta ttacaatatg ctaaaggtct
    61861 taatacacag cagtatttga aatggcatcc agcaggaagt cttggaaagt ctattagaga
    61921 gggaatataa ttttatgaaa tataaaggga ttatatgtgc aatgataaca ccatttgatg
    61981 aaaagcaaaa tattaattca caagcaacat gtaaattaat tgattatttg attgaaaagg
    62041 ggatttatgg attatttatt ttgggtacta atggtgaatg tcatgtgtta acagatgatg
    62101 aaaaagttga atttgcaaag attgttatta atcatacaaa taatcgtgta cctgtttttg
    62161 ttggaacagg tggtaattca actcgtgaag ttatagattt atccaagaga atggaagcga
    62221 ttggagcaag tgcattatct attattacac catactttgt tacaccaaca caacaggaat
    62281 taatcttaca ttacaaaact attgctaatt cagttaattt acctattata atgtataata
    62341 tgccaggtaa gactggaatt aatattgaac cagagtcagt acgtgaatta tcaaagataa
    62401 agaatattgt tggaattaag gatagtagtg gtaaacttga caacatgaaa gcttatattg
    62461 aagtaacgca gaatgagaat tttagtgtgt tttcaggctc agattcactt attctggata
    62521 gtctaaaagc tggtagtcaa ggagctgttg cagcaacaac aaacttcctc acagagattg
    62581 gtatttctat ctatgaaaac tttatgaaag gggatttgga agaagctcaa aaagctcaaa
    62641 gtagtataga agaacttcga cgtatactaa aacttggaac aattccatct gttatgaaga
    62701 aaactattgt cttgaatgga attaatgttg gaactgcacg acttcctgta actgaaccaa
    62761 ctggggaagt acttgaagaa attaaacgag tagttgaaaa ttataggaca atacttaata
    62821 aaagtagtga gaatagataa tataaatagg gaaattacac tggtatataa ttaatatata
    62881 tattataaaa catgactaaa attaatgaat aagtaaagtg gtttgatgag tatagaggtg
    62941 ataatatgaa aaagatgaat ataaccaaaa gaatgtcaga atgtggggct cttgcaattg
    63001 taagagaaga aaatcttaac cgtgcatgtg aaattgctga aggatgtata aaaggtggta
    63061 taacagttat agaaatgagt tataccctaa ataatgctgg agaaataatt caagggctta
    63121 ataaaaagta tggtgaaata ctttgtgtgg gagcaggaac agttttggat agtgaaacag
    63181 ctcgtcatgc tatattacat ggagctcaat ttattattgc accaaattac aatgaaggtg
    63241 tagcgaagat ttgcaataga tatcagattc cttatgctcc aggatgtaca tcattgactg
    63301 aggcagtaga tgcactaagt ttaggagcga cttttataaa agctttccca atatcagatt
    63361 tctatggacc taaactagta aaggtattta agacaccaat acctgatatg ccagttttag
    63421 caagtggagg aattaatctt gaaaacctac atatatggtt agaaaatgga gttgatgtat
    63481 gtggatttgg tggtttatta actaaaggaa ctattgagga tattactgaa aatgccaaaa
    63541 agattcgtga aatcattaaa aatataagag aaatttaaca tggaggatga attagttttc
    63601 tattgacgag atatccacac attaattcct aagttagaag aaatattttc tagttgaggt
    63661 aaagtttata ttgaacttaa attaaatttt gttttaaata agatattatt ttagttgatt
    63721 gctaaaaata ctagaatcta tattaatgaa ttatactaat tttgtaccac tattaaaata
    63781 ttgtttgcta aaaataaaaa ataaccttaa aacagttgat tataaaaaat taactgtttt
    63841 aaggttattt ttatctttgt gacgaaatag acacttgcca tttagaatgt gacaatatag
    63901 tagttgttta gaaaaggtac aaagcattat actttaacta aagtaaaata tattttgctt
    63961 ttagaataaa aacaattagg agggagctat aattgaaaaa gataatatat gtattattta
    64021 caattgaact attaatagta tctttattag gtctaaatat agttaaagat aatgaaatta
    64081 ataatatttt atataacgat acaacaagta tatctgtaat gtttaaagat tataaaaagt
    64141 taaataaaaa ttatagtaag tggattaaag atattgcaga tgaaaataat gtggctatat
    64201 caaagtatgt ttttaataat aatgaaaaat tatctatata tacgactgat acaagtttga
    64261 ataacaatat aaagttaaaa tcaggaaaat ttcctaatac taattcaaat gaatttatta
    64321 gtaatataaa cactaataat agtaataata gtaatcaagt aggtaaattc tcaactatga
    64381 gtaaagacat atcaatttta attagggatt ttggtaaaat aagtgaagtt ggagaatcag
    64441 gaatcttata tatttcaact caaaatgaag atacaataaa taaaatatta agagaactaa
    64501 atgtagacag aagtattttt gtagcaagat tacatgatag atatgttaac tcaaatttat
    64561 acttaaatcc ttcaataatt agagatttaa tactaattat cctttgtttc ttggctacaa
    64621 taatacatta ttcaattagt gtgagtagag aggcttctat tttaagatta aatggatact
    64681 ctaagctaga tataattgct agggttataa aaaatatatt tagaattatg attttatcat
    64741 ctgtcacagc attattaata tatataatct atattttaaa attaaatata ggtgttagag
    64801 catgcttata ctttgctatg ttttcgatta tatgtatttt gataaatatt ttgatatcta
    64861 tattaatagt tggttttaat agtagaagtt ctaaatatgt gctatctcta aatggaaaaa
    64921 aatcttatgg ttttgtaaat ataatgcatt tcacattgaa aattgtattt gtatcatttc
    64981 ttatattatc cataaataac tgtattttaa attataatat gctacaaact cagttaggaa
    65041 atctatctga gtgggataaa gcaaaaaatg tttataatct tactcttaaa gatacaggcg
    65101 aacaatcttt aggaaaggaa gaagtaatta aaaatgccaa gattaaaggt ttctataaaa
    65161 atttggtaga agaaaaagat gctttcttaa tagattcaac taattttgaa aaacttgaag
    65221 atggaagtta catgtatgaa ataaattcaa agggtaaaaa cccagaagta agtcaatatg
    65281 gtaaaaatat aaaaataaat aaaaactatc ttaaagttaa ccctatttac tcaaatggca
    65341 aagaaatatt taatctaatc gattatgacg ataagactat aaatttatta gttcctaaaa
    65401 agttacaagt acatgaaaat gagattaaaa aagagtttag agaattattt tattttgaaa
    65461 aaatagaagt agaaaatatg tacaataaag agttaaatag agagttaaat aaaacaaaga
    65521 agtcagattt aaatgttaat ataatatatg tagataataa tcaaagctat tttacttata
    65581 actcccttgt tatggatgac aataggaatc tcattgatga cccaatagca ataattgaca
    65641 tagataatat tgatgattct ttttatcttt cttatatatc tagatgtgta tattttaatt
    65701 ctaagaaatt agatgccctt gcagatataa gtaacattat tgaagctcaa ggtgtagaag
    65761 cgcatatcca aagtttacat gctgtttata atgagtatgg tttagaaata aataaactag
    65821 aaaaatattt gaatagtgaa atttttacta taataataat agcaatatca aacttaatga
    65881 ttacctacaa tatagtagct agctactatg aaagaaataa atataagtta tatattaaaa
    65941 aaatatttgg ttattcaact acagctagaa gtatgttgtt aatagtctca ttaatattaa
    66001 caaacattat tccaataggc attatatcaa caagagtaga tttaagtaat aatattatct
    66061 tatttggact tctgatatta gttatagaag taattgtatc tattgtatta gacaagatta
    66121 tctcaaatag ttcatttaat aaaattataa aaggagaaca ttaaaaagat gatttcatta
    66181 ataaatatat ctaaaaaatt taaagataaa tctataatag acaaatttaa cctagaaatt
    66241 tctaagggcg agtttgtagc gataacagga aatagtggtt caggtaagac aactttactt
    66301 aatataatgg gattattaga aaaacctaat tctggtgata ttataataaa taatataaaa
    66361 aatccaaata gtaaacagat aagaatttta caaagggatt tttatggcta tctatttcaa
    66421 aattatgctt taattgagaa tgaaaatgtt gaaaataact tgaaaattgc tttaaagtat
    66481 caaaaaaata tcaaccataa tgaaaaaata aattacgcac ttgagtctgt aggtcttaga
    66541 aaatacaata aaaaaaagat atacgaattg agtggaggag aacaacaaag ggtagcatta
    66601 gcaagagtaa tattaaaaga atgtgaagta atatttgcag atgaacctac aggtaattta
    66661 gataaaaaaa atagagacat agtatttgat atacttaaaa atctaaataa gaatggtaaa
    66721 actatcgtat ttgtaactca tgatttagag ttatcggagc aggcagatag agttataaaa
    66781 ttgtaataat atcaactata ataaatataa gaaagagatt acagctaaat caagtttaat
    66841 aataatacga atagaattta aacattaagt aaattagtag tttgtaaata taagtataat
    66901 atgatatcat aaaggctcaa aagaaatttt atataataag aaatttaaaa ataacaaatg
    66961 gaggacatat aaagatggaa aatttaccaa attgcccgaa atgtaattca gaatatactt
    67021 atgaagatgg aactctttta gtttgtccag aatgctctca tgagtgggca ttaggattgg
    67081 gagctgaaga agattcaaat attatcaaag atgtaaatgg aaatgtatta aatgatggag
    67141 attctgttac agtaattaga gaccttaaag taaaaggaag ttcatcatct ataaagattg
    67201 gaacaaaagt gaaaaatatc cgtttaatcc atgactcatc agatggacat gatatagaat
    67261 gtaagataga tggttttgga gctatgaagc taaagtcttc tgttgttaaa aaagcgtaat
    67321 attataaata agctgtagtg tttattgttt ttcagtacag tataaaacca taagaatcta
    67381 ttaagtaata aaagtttgtt tatttatctt tatattttct tatggtttta ttaaatataa
    67441 attttattga acaccaaaat attctctcct ctacaaataa gccaatctta tttaaattaa
    67501 tatactttgt actatttata ctgaaattgt gaatttaaat gctatgttat ttaggtttgt
    67561 atagttaatt tat
//
LOCUS       DAMJWP010000004        63450 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.004, whole genome shotgun sequence.
ACCESSION   DAMJWP010000004 DAMJWP010000000
VERSION     DAMJWP010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 63450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63450
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.004"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            115..990
                     /locus_tag="PC700_000228"
     CDS             115..990
                     /locus_tag="PC700_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine DNA glycosylase"
                     /protein_id="HDF2579556.1"
                     /translation="MNVYEKGNGVILEGVTDFDPVHIFECGQCFRWHKQEDGSYTGVA
                     RGRILNVKKENDKIYLNNTNLKEFNSIWYNYFDLGTDYTEIKNKLKNMDEYLNKATEF
                     GWGIRILRQDGWEMLISFIISSNNRIPMIQRAIENLSRKFGKYIGEYEGNEYYAFPTP
                     EELNKASQEEIRACQTGFRDKYIKSTTQAVIENNDEVSEYTNLSTEDCRKELLKFNGV
                     GPKVCDCIALFGMQKYDSFPVDVWVKRVMQEFYIDEDMSLPKMRTYGIDKFKEMSGFA
                     QQYLFYYARELGIGK"
     gene            1008..2498
                     /gene="cls"
                     /locus_tag="PC700_000229"
     CDS             1008..2498
                     /gene="cls"
                     /locus_tag="PC700_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDF2579557.1"
                     /translation="MGVIGTIFLFYLIISYLAGAIISVIILLENRDPAKTMSWLLMFI
                     IFPGVGLMIYAISGRNIRKRKLFKTQKLANNIKEKKLFDTLEKITEIVELEKESIKQN
                     KLLRDEEDGSYRKRVINMLLKTGMFPFTKNNKVDVFVDGNEKFKRLIEDIREAKDHIH
                     LEYFIIKDSEIGRVLKEELIKKAKEGIKIRILYDDVGCWRFWFNRKFFREMREVGIEI
                     AAFLPTKFPIIGGKLNYRNHRKIVVIDGIIGYTGGINIGDEYLGKNDKFGYWRDTHIR
                     IKGISVYMLQMTFLIDWYYTTKEVLVTKNYFPSVGNVGESMIQVVASGPDSDWEDIHY
                     AYFSAICQARKNVYIETPYFIPDESLLKAIKSAALSGVDVRIIFPKIADHKIVNIASY
                     SYFEEILRAGGKVYLYNKGFIHSKVVIIDDKIASAGTANMDLRSFMLNFEVNAFIYDE
                     EVIRVMTDDFFEDLSHCEELNLEVFKNRNIIQKIKESVARLFSPIL"
     gene            2691..2975
                     /locus_tag="PC700_000230"
     CDS             2691..2975
                     /locus_tag="PC700_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDF2579558.1"
                     /translation="MKIRPLADRVVIKKVEAEEKTASGIVLPGAAKEQPQIAEVVEVG
                     PGGIVEGKEIKMELTVGDKVIFQKYSGTEVKIEGQEYTILRQSDVLAVIE"
     gene            3008..4636
                     /gene="groL"
                     /locus_tag="PC700_000231"
     CDS             3008..4636
                     /gene="groL"
                     /locus_tag="PC700_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729227.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDF2579559.1"
                     /translation="MAKEIKFSEETRRALEAGVNKLADTVKVTLGPKGRNVILDKKFG
                     SPLITNDGVTIAKEIELEDRFENMGAQLVKEVATKTNDVAGDGTTTATVLAQAIIREG
                     LKNVTAGANPILLRKGIQKAVIVAVEELKNQSRIVETQEAISQVASISAGDEEVGKLI
                     AEAMEIVGKDGVITVEESQTMNTELDAVEGMQFDRGFVSAYMVTDVDKMEAVLNDPYI
                     LITDKKISNIQELLPVLEQIVQQGKKLLIIAEDVEGEALSTLVVNKLRGTFDVVAVKA
                     PGFGDRRKEMLQDIAILTGAQVISEELGYDLKEADLSMLGRASSVKVTKESTTIVDGS
                     GDKKAIEDRVTQIKHQVEQTTSDFDREKLMERLAKLAGGVAVVKVGAATEVELKERKL
                     RIEDALNATRAAVEEGIVAGGGTAFVSVIPAIGTLIESLEGEVKLGAQIVKKALEEPL
                     RQIAINAGLEGAVIVQNVVNSEAETGFDALNEKYVNMIEAGIVDPTKVSRSALQNAAS
                     IASTFLTTEAAVADLPEKEDAGMPGMGGGMPGMM"
     gene            4898..5638
                     /gene="larB"
                     /locus_tag="PC700_000232"
     CDS             4898..5638
                     /gene="larB"
                     /locus_tag="PC700_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HDF2579560.1"
                     /translation="MDLRKLLEEVKNENIDIDFALEKLKDLPYEDLGYANIDHHRELR
                     NGYPEVIYCEGKSDEHILGIIDKMNQKSSNILGTRCRKETFLKIKEIYNHAEYEEASK
                     ILKIQNHDIENIGKGKIVIATGGTSDIPVADEAYHTAKFLGNDVDRIYDVGVAGIHRL
                     LNKRHKIDSARVIVAVAGMEGALASVIGGLVDVPVIAVPTSVGYGANFGGLAALLAML
                     NSCASGISVVNIDNGFGAGYLASMINKL"
     gene            5722..6456
                     /gene="larC"
                     /locus_tag="PC700_000233"
     CDS             5722..6456
                     /gene="larC"
                     /locus_tag="PC700_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HDF2579561.1"
                     /translation="MSEKILYFDIISGISGDMTLASLLNLGVPKEIFLEEIHKLRMSD
                     EFNINISSKTENGIVGTKVEVITKEKHTHRNLVDIFEVIDKSTLNENVKSKAKKIFMV
                     IGEAEAKVHGTTIDKIHFHEVGAIDSIVDIVGASILVDLLCVDKVCASTVSVGSGFVK
                     CEHGIIPIPAPATIEILKGVPIKLNNVNGECTTPTGAAIIKVLCDEFVDELEFSPKQI
                     GYGIGHKKFEVPNILRTVLGEKKKKN"
     gene            6486..6905
                     /locus_tag="PC700_000234"
     CDS             6486..6905
                     /locus_tag="PC700_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF111 family protein"
                     /protein_id="HDF2579562.1"
                     /translation="MSSEIYSYLYEKILDEKALDIYTESIYMKKNRPATKVSILCNES
                     DLEKFIKILLLETSTFGVRYNAYNREILERKFVKLDTKYGVVTVKLGYYKGKLVKVTP
                     EYNECKTIAKNEDIPIKKVFADINFRIKEEFSENLLT"
     regulatory      6965..7066
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            7287..8822
                     /gene="guaA"
                     /locus_tag="PC700_000235"
     CDS             7287..8822
                     /gene="guaA"
                     /locus_tag="PC700_000235"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDF2579563.1"
                     /translation="MKHELVLVIDFGGQYNQLIARRVRENNVYCEILPCTASIERIKE
                     KNPKGIIFTGGPNSAYLEDSPTISKEIFELGVPILGICYGIQIMSHVLGGVVRKGNKQ
                     EKEYGKTSITYGKSSLFEGITTNSVWMSHTDLIEKVPEGFTIVANTNDCPVAAMENVE
                     RNLYGVQFHPEVEHCLEGDKILTNFLYNICKVKGDWTTDSFIEDKIKELKEKIGDKKA
                     LCALSGGVDSSVAAVLIHKAIGDNLTCIFVDHGLLRKNEGNDVERIFREKFDINLIRV
                     NAEDRFLSKLKGVSEPEAKRKIIGEEFIRVFEEESNKLGKMDFLVQGTIYPDVIESGH
                     GNAATIKSHHNVGGIPEDVDFQEIVEPLRELFKDEVRKIGLELGIEEGLIFRHPFPGP
                     GLGIRVIGDVTKEKCDILREADAVYMDELRKAGLYREIWQAFATLPDVKTVGVMGDER
                     TYAYLVGLRAVTSSDGMTSDWYKMPYDVLERISNRIINEVDGVNRVVYDITSKPPGTI
                     EWE"
     gene            9361..10791
                     /locus_tag="PC700_000236"
     CDS             9361..10791
                     /locus_tag="PC700_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2579564.1"
                     /translation="MILNQIFIEDGGIMRNKIIIGITFSLVLLMSGCASKQTAEINVL
                     ATTDLHGVIPYELTSYVKEERKKDKNLTLVDAGDFFDSGEVFGSSMDSYFSARYKDGD
                     NNTESYIETPISKDMKEVGYDVAVLGNHEFISNNKFYLDNMISDFEKQKINILSANTY
                     KKNGDSYTKPYIIKNINTPEGNVKLGILGLTIKEVGERKQWKDGKLVDSKSLELKDQE
                     GYNGELYMNDLVEDAKKWVGIMEKEKPDIIVAVAHTGEKPKKPRNPGNRIQDLAQQVD
                     GIDAIVAGHNHVQIEQHDYKNKSGENVIVTEPGKHGEYISKINFKLEKNNDKWNIVDK
                     SSKLIKFEKNQESDKFGEFISSINKVSKDKKEIRLSEVKTPEWDKIYVFKSGTSADDI
                     YKTIGYKWRNISSPKGDDMVQIVVMNNNKPVSYIYGEAEVMPINIKCDESEYKNNVVT
                     IYPNKNDKFKIEKGKQEYSINLKHIQ"
     gene            complement(10939..11814)
                     /locus_tag="PC700_000237"
     CDS             complement(10939..11814)
                     /locus_tag="PC700_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2579565.1"
                     /translation="MINIRYLEILEAIEQTGTFTGAAKKLHITQSAVSHAIAELEQQA
                     ETSLFDRLPRGVCLTRCGISLLDEARSILTACRNLDKKISRLEECTPINIVSSITIAS
                     FLLPPILSRLKNSFPSLKVNVRVESANSAMDILKSGEADICFWEGIEPQGAFRTILLG
                     SYKLCVACAPTFPLSEQTLSLRQLCTFPLLLREQGSAIRDTLDSVLSLSNQKAYPVWE
                     SVNSFALIKAAEAGLGITILPENLLLDSLLHKKLRLIELDEIDMENKMLAILHKDKNI
                     TQPLEIILDNISNVL"
     gene            11934..12497
                     /locus_tag="PC700_000238"
     CDS             11934..12497
                     /locus_tag="PC700_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HDF2579566.1"
                     /translation="MNQSRKIYFWLLIINLFISTFTFGGGYVVVPMIRRYFVNQKQFF
                     TEDDLMSMAAVAQSTPGAIAINLSALAGYRVAGTLGVVISCTAAIIPPLVILGLVSAF
                     YTTFISNAIVTAVLKGMQAGVAALIVDLIIDMCSMILKQRSLFLSTMIPIAFFANFVM
                     SINVIFILSVCCFVCVMQVFYKKRENK"
     gene            12494..13060
                     /locus_tag="PC700_000239"
     CDS             12494..13060
                     /locus_tag="PC700_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HDF2579567.1"
                     /translation="MNTIWNLFFIFLKIGLFSIGGGYAIIPLIQEKVVNQTGWISEKM
                     FTDIITISQMTPGPLAVNTSTFVGLQIGGFWGAVAATLGCVLCGVVISLFLYNFFQKH
                     QNSTYIFEILNGLKSASLGLIISAAATIIILALFGTNKVYFNSIFEMLNWTALIIFFV
                     ILLIVRKWKINPIVIIIISGILGIFFYL"
     gene            complement(13189..13425)
                     /locus_tag="PC700_000240"
     CDS             complement(13189..13425)
                     /locus_tag="PC700_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2579568.1"
                     /translation="MLECFIAEDSKIKDGVNIPTLVFKTLHDRYFKTQYFDSYYNSLK
                     CKKKLVEINNVHNSYYIDSEKFCQEAYDWFVENQ"
     gene            complement(13735..17163)
                     /gene="uvrA"
                     /locus_tag="PC700_000241"
     CDS             complement(13735..17163)
                     /gene="uvrA"
                     /locus_tag="PC700_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF2579569.1"
                     /translation="MKINNYKNQSIITNPKKFENKYQDLPKTPIELLKVVQSLVIHGD
                     QGKLYGISFNKQQSDEELLRTIPQMLKRIFEINSNPLTIPRNPKQRLVGMCRDYSLLL
                     VSLLRYRGFEARMRAGFANYFENELTYEDHWLVEYYDTLTKRWIRIDAQIDDIQKNYF
                     QINFDTHDVGKTDGFLTGSEAWIRCRQGHAHPDDFGYNKNWKGWHSVKGNLLHDFNNM
                     IGLELLPWDLWTELSSKKYNQLTRAEKNLLDEMAEILSSGNIKIEDLNLLIEKLPEDY
                     LKSIFSQLKILGISEIKELGNPLELEKKFKFAKAINKSIKNSLCHNKSSIYLKGGRQN
                     NLKDVEVTIPKNQITVITGVSGSGKSSLAFDTIYEEGKRRYFENLGNGAKLSEQLQKP
                     EFDLLQGLTPTIAIEQKKGSQNPRSTVGTLTSIWDYLRMLFVSIGKSYCPYCKTPLEK
                     KNNTKNYCPHCQTIFSKINTSTFNANSHTGACHDCNGLGFTYQVNPQLIVKDPTISIL
                     DGATYYFGKLRGKKPNGNWMVGELYAIAKDKNIDLDIPWNELPRDFIDAILYGTDDKI
                     YEFSFESKGRESKIRRPASGAINHIQRLFRESSSENNTLHQYMNKIPCNTCGGELLCI
                     EARFTTIKGYRFPELTKMTIEQLWNWLCELPNQLQKNELSLVNDILTELKIRVSYLLK
                     VGLSYISTDRTAPTLSGGELQRVRLSSQLGSELVGLTYILDEPSIGLHPRDHNLIIKM
                     IEELRDKGNTVIVVEHDKDTILSADYIIDVGPNAGTKGGFIIAEGTTQEIIKNPNSIT
                     GKYLSTYNKISSQNKIIPSKWLSLKGCHANNLKNIDVEIPLNCMCSITGVSGSGKSSL
                     VFHSLLPALEEKLKQKSIPNKNYTALTGFDAIDDFILMDQTPIGKSSRSTPATYINIF
                     DEIRSLFTETPQAKQKLLDESYFSFNSKKGQCPNCQGLGKTKIILQYMADQWVTCNEC
                     QGKRYQKEILSIQYKGKTIADILDMEVAEAKTFFSDCSDIYRKLSLLDEVGLGYLKLG
                     QNTLGLSGGESQRIKLAKELGTKTKKRMLYILDEPTTGLHFKDIENLLITFRKLVNEQ
                     HSLLIIEHNTEVIRASDWIIDIGPDSGINGGEIVASGTPDEIKINPNSITGHFI"
     gene            17482..19716
                     /locus_tag="PC700_000242"
     CDS             17482..19716
                     /locus_tag="PC700_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2579570.1"
                     /translation="MKLSRYKKNSLVLSIILCSLIISILVITVFNTVKLHNISNKQSK
                     NYLNDVSTQIVMNVDSKIKFILSDLRIMADFIKQYEGDSRYEYLSKRKTSYKYYDVGI
                     IDLEGKAEFLSGKKFDLKNTASYKKGIEGKEYSEVIESMGFVLYSVPIFNDNHEVSSI
                     LIGVSNKESMDEILNIDSFNGEGTIEIINSQGKTLFIGKNNELIKDLSYKYKDSADEP
                     WAQKMLNDFKNDKSGNITITSSKGVQCLLTYHPITRGLNDWHFLLIVPEDVVLGELNK
                     LNSFTIIMTFITTCIIGIITLILYIIRRKYVNQIENIAYLDSITNGINSTKFSMLVKP
                     LISESPDSTYMMIAMNIKDFKLINDCFGSEKGNMTLKHLYNILYKNIDENEEFVCRHD
                     ADLFYLLIKNRPVLEALELLYKIEADTNYFNKNRENPYFLRLSVGIYTIENHNEDLIT
                     IQDHANTARKSFNKTHQSDFSFYSDIERKRLISEKEISNLMEKALENKEFFMCLQPKI
                     DIKSGKISGAESLVRWKNSEKGIIYPSNFIPLFEKSGFICKLDLYILEETCKLISKWI
                     KEGKEVLRISVNVSRQHLNDKLFLEKYKHICNKYNVPTCLIDLELTESIFLENPEAID
                     IIEDIHSNGFKCSIDDFGFGYSSLGILKDFKVDIIKLDRSFFVSKNNIDRGKVVIKSI
                     IELSKRLGMKVTAEGIEELEQVEFLKNVGCDYIQGYVFSKPLLISDFENFAYENNKIK
                     NIHY"
     gene            19972..22002
                     /locus_tag="PC700_000243"
     CDS             19972..22002
                     /locus_tag="PC700_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2579571.1"
                     /translation="MISGRMLLIMNFLKGKNKTSYKDISKQLSIEERKVRYDINNLNI
                     VLRSLNKSVIQKMNKGVLIIPDDFQVVCINNNEYVFSLTERVSIMKIISMFKIDNLNL
                     EKLSKGFQVSRSSIKNDLNVLSSELERNQITITYDKSFKISGEEDLIYKQRLNILKSY
                     SYLFGKEKSDLSVFEKYLVNVIEKIFKGIYLIDIYNWSMNLLLQMGWTLNDESFTWYV
                     SNIFLFTWYIHSDEKHPLESASLSEPCFENKFDQEFETIIQKKLTKEQIFLLKSFVFF
                     TNKYASLNEDMDLISTEIIVQDLIYNMSEILEISFKEDMILYKGLLNHIAPLIERIKQ
                     NIQIYEELLHVIPDKYQYVLEATKVSIQKNRVLSQIKNENEIILLAIHFLGSIHRNER
                     NNYKNILLVCGFGYGVIAMLKDSLVNDYQINIVESIPSYKLQDYKEWESIDFIITTSK
                     IVIDLPKTIIEINPFLQEEDYLKFEEKGIKRKNVLTNYMSIKKRLDFLEEDAQKRVLS
                     IICQELGYSDERILFRQLNLSDLIGIDTIRIIDEDMKWEDAVKISSNILANCDFVSAQ
                     YAENIIDILKNVGFYAVKDNEFALLHGNDSSLVKVSSISLLICKEAVSFGDKKVKIIF
                     CLASRDKKEQIPAIINLTKMVYKTNFIAMLESAKTKEEAEVLIHKYEKEVTV"
     gene            21999..22451
                     /locus_tag="PC700_000244"
     CDS             21999..22451
                     /locus_tag="PC700_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2579572.1"
                     /translation="MNKLVNVKCSFKIHANSKEEAIVSLVDVVSKEGYLIDKNQFLED
                     VLKREETLSTYIGHGIGLPHSQSVGVKNSCITIGKLDIPIEWTEEGEKVDLIFLISVT
                     KDNENNLHLKILSKLARLLMHESFRNQIRCSDEQTVYNLIKEKIEEED"
     gene            22453..23508
                     /locus_tag="PC700_000245"
     CDS             22453..23508
                     /locus_tag="PC700_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HDF2579573.1"
                     /translation="MRILKFLQKHLMTATSYMIPFVVAGGILFALSVTLSGQAAVPET
                     GWLAKLNQIGAAGLALFIPILGGYIAFSMADKPGLAPGMIGAYLAKEVNAGFIGGIIA
                     GFIAGFVVLQLKKIKLAPTMRTLGSIFIYPLIGTLITGGIIVFLIGEPIASFMTWMTN
                     WLNGMSGVSKIPLGGILGGMIASDMGGPINKVAATFAQTQVDTLPYLMGGVGVAICIP
                     PIGLGLATLLFPKKFSKEERDSGKASLLMGCVGITEGAIPFATSDPVRVIPCIMAGSI
                     IGNIMAFLLGCLNHAPWGGLIVLPVVDNRLGYIASVLTGAVVVAVLMKLVKKDVKEDE
                     EIEEDLDESIELIFEEL"
     gene            23575..23883
                     /locus_tag="PC700_000246"
     CDS             23575..23883
                     /locus_tag="PC700_000246"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HDF2579574.1"
                     /translation="MKIVAVTSCPSGVAHTYMSAESLELSAKKFGIEIKVETQGSSGI
                     DNELSLKDIDEATCVILTNDVEIRNMERFKGKKVVRMSVSEIIKKSDALIKKIKDTFQ
                     "
     gene            23987..25252
                     /locus_tag="PC700_000247"
     CDS             23987..25252
                     /locus_tag="PC700_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HDF2579575.1"
                     /translation="MKKLPIKTVVQRLLDLQEEGKSATLLGIGPMSPNLLQASFELAK
                     DDDFPLMFIASRNQVDADELGGGYVNGWNQETFTKDIKKIADKVGFDGLYYLCRDHGG
                     PWQRDKERNDHLSVDEAMELGKKSYLADIEAGFDLLMIDPTKDPFEIGKVIPLDVVLE
                     RTVELIEYCENERKRLNLPDIGYEVGTEETNGGLTSTETYETFITRLKVELDNRGLPM
                     PTFIVGQTGTLTRKTEQVGTFNFRNAYDLAQMAKKYGVGLKEHNGDYLDDVTLLEHIP
                     SQIIATNVAPQYGTEETRAYLKLAEVECKLEKEGLVDKTSNIRHVLLVNAIECGRWRK
                     WVVGEQKNLTTEEIFKNEVLSNEILDIAGHYTFNNDDVKKEIEVLYDNLSKNNIDGQR
                     FVVDHIKRPLRDYAECYNLKGVTTRILNK"
     gene            25340..26056
                     /locus_tag="PC700_000248"
     CDS             25340..26056
                     /locus_tag="PC700_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDF2579576.1"
                     /translation="MINLIFDVDDTLYNQLTPFYTAYNKVFSSIKDISIEDLYMSSRK
                     YSDEVFHMTENGEMPIKEMHIYRIMKAFEELGNSITEKDAQSFQDEYIYQQSQITLIP
                     EVERILNFSKERNINLGIITNGPSNHQRMKLKQLNIENWVDKSNIFISSEVGFSKPDT
                     NIFRVAENVMNLDRENTYYVGDSYRNDVLGAKKAGWKSIWLNHRGHEVEELFYKPDFV
                     ILEHKDLISLFIKICSYKNM"
     gene            complement(26251..26544)
                     /locus_tag="PC700_000249"
     CDS             complement(26251..26544)
                     /locus_tag="PC700_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HDF2579577.1"
                     /translation="MRNMIENLIKNNTYIDDKVIMLSMLSSAKASANMYLNSALTSST
                     PELRAIYSASLTQMVEGHTALTELSINKGWVKPYDKPIQQLTCSYKESQNVID"
     gene            complement(26550..26765)
                     /locus_tag="PC700_000250"
     CDS             complement(26550..26765)
                     /locus_tag="PC700_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2579578.1"
                     /translation="MRTLSETEKISLAAVIKMESDGLLMQRAINMLISDEDLKRQSES
                     SILATEGRIKAIQQFIVENEILISEEV"
     gene            complement(27310..27822)
                     /locus_tag="PC700_000251"
     CDS             complement(27310..27822)
                     /locus_tag="PC700_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2579579.1"
                     /translation="MLEVIKNRRSIRTYIDKNIEEDKITEILKSAMQAPSSKNAQPWE
                     FIIVDDKELLKELSKSQHRAKHIEFAPLCIVVLGNRDKFLKPGKWIQDLGACTQNLLL
                     EVTNQGLAACWAGVFPKNKVVNKVKQILDLPVKLVPYALISIGYSEEKNEFIDRFDEN
                     KIHRNVYKNR"
     gene            28272..28748
                     /gene="purE"
                     /locus_tag="PC700_000252"
     CDS             28272..28748
                     /gene="purE"
                     /locus_tag="PC700_000252"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDF2579580.1"
                     /translation="MKVAVVMGSKSDYPKLEEGIKLLEKYGIEVVARALSAHRTPEQL
                     SIFLKEIEDDTDVIIAAAGKAAHLPGVIASQTLIPVIGLPIKSSTMDGLDSLLSIVQM
                     PKGIPVATVTIDLGLNAALLALQIMTLKYPKLKEDLKSYREEMAQKVLEDDKNLRG"
     gene            28754..29452
                     /locus_tag="PC700_000253"
     CDS             28754..29452
                     /locus_tag="PC700_000253"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDF2579581.1"
                     /translation="MLLYEGKAKQVYSTDNENEYVVYYKDDATAFNGEKKAEISSKGI
                     LNNKISTIIFEMLKENNINTHFIKSLSDREMLVKKVEILPLEVIVRNIAAGSICKRVG
                     LEEGVVFDEPIFEISYKNDAYGDPMLNDDYAVAMKLATREELKFLREETLKINELLKA
                     FFLKLNLKLVDFKIEFGKDSEGNIILADEVSPDTCRLWDVNTNEKLDKDRFRKDLGDL
                     VEGYTEVLSRMNNK"
     gene            29464..30831
                     /gene="purF"
                     /locus_tag="PC700_000254"
     CDS             29464..30831
                     /gene="purF"
                     /locus_tag="PC700_000254"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDF2579582.1"
                     /translation="MCGVLGIYSNKDVTKELYYSLYSMQHRGQESCGLALLDDGEIKY
                     KKDMGLVGDVFKENELSKLKGNIGIGHVRYSTAGGSHVSNCQPLVGSCRKRQLAIAHN
                     GNLVNANYLKDMLEEDGYMFQTNSDTEVILYILARYYKGDIVESLKVTMDYIKGAYAL
                     VIMSQEELVAVRDPHGFRPLVLGKKGDEYIFASENCAIDILGGEVIRDVEPGEIIVVK
                     DGELKSYFYSENYKPVKKSCIFEHIYFARNDATIDNVNAYEFRIKCGERLAQNETVKA
                     DMVVPVPDSGWPGAIGYANASGLKISEGLVKNRYVGRTFIKPTQEEREIAVKIKLNPL
                     STIIKGKSIILVDDSIVRGTTSKQLVKSLREAGAKEIHLRITSPPVAYSCYYGIDTPN
                     RSKLIASSNNVEEMREYIGCDSLKFLDIEGMLDATEHKSTFCKACFDGEYPVKKIDKE
                     ELLSC"
     gene            30825..31901
                     /locus_tag="PC700_000255"
     CDS             30825..31901
                     /locus_tag="PC700_000255"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDF2579583.1"
                     /translation="MLTYKESGVDIDEGNRAVDLIKGKIKGTYDGNVVGDLGNFSGLY
                     SLKDFVGMKEPVLLASTDGVGTKLKIAQMMDKHDTVGIDLVAMCVNDLICQGAKPLFF
                     LDYIALGKLVPEHIEKIVGGIADGCKMSGCALIGGETAEMPGMYGEDDYDLAGFSVGI
                     ADKEKIVSGNNVKSGDVLVGISSSGVHSNGFSFIRKIFLETYNYKMEQYVEELGMTVG
                     EALLTPTKIYVKLALDVLAKHDIKAIAHITGGGLIENITRVIPKGLGLDINKKSWEKP
                     PIFKMIEGFNAVDERELHKSFNMGIGLVLIVDKENADDVVNFINNRENDNAAYVDKKY
                     SELLEDKAYIIGEVVDSHEGVELC"
     gene            31895..32488
                     /locus_tag="PC700_000256"
     CDS             31895..32488
                     /locus_tag="PC700_000256"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDF2579584.1"
                     /translation="MLNIGVLISGGGTNLQAVIDGTESGEIKGQVKVVISSKQGAYGL
                     ERAKNHNIKAICETDEDKIIEILKENKIDLVVLAGYLKIISPKLVNEFRNKMINIHPS
                     LIPSFCGAGFYGEKVHQGVIDYGAKVTGATVHFVDEGADTGPIIMQDVVKVNQDDDAK
                     TLAKRVLEVEHRILKESISLFCENKLKLQGRRVFINE"
     gene            32481..34013
                     /gene="purH"
                     /locus_tag="PC700_000257"
     CDS             32481..34013
                     /gene="purH"
                     /locus_tag="PC700_000257"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDF2579585.1"
                     /translation="MSKRALISVTDKTGVVEFAKELNKLDYEIISTGNTFKTLKENGV
                     NVMQVEDVTNFPEILDGRVKTLNPYIHGGILYKRDKESHVETVNEHKIHSIDLVAVNL
                     YDFEGTLKAGKSHDEIIENIDIGGPSMIRSAAKNYKDVIVVVDIKDYDSIIEKLKTDT
                     MTLEDRKKLSYKAFSTTGRYDALISSYFAGEVGDTYPDILNLTFQKEQTLRYGENPHQ
                     NGFLYSQSNAKNPILNYEQLGGKELSFNNLNDLHGCLEVMREFKDSEEVVSVAIKHAN
                     SCGVGLGKDAFEAYTKCYEADKVSIFGGIVGITSTIDKATAEKLNEIFLEIVVAYDFE
                     PEALEVLKQKKNLRILKLAKIEDSLQPYEMKYLDGKLLIQDRNNILAEKSENVTKAKP
                     TDAQLKDMEFGMRVVKNMKSNAIAIVKNGQTLALGCGQTSRIWALKNALENNKDKDFT
                     GAVLASDAFFPFDDCVTLAHEYGISAVVQPGGSIKDKDSIEACDKYDMVMVFTGIRHF
                     KH"
     gene            34024..35274
                     /gene="purD"
                     /locus_tag="PC700_000258"
     CDS             34024..35274
                     /gene="purD"
                     /locus_tag="PC700_000258"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDF2579586.1"
                     /translation="MKILVVGGGGREHAICWKLSKEKNVEKVYCAPGNAGITNVAECV
                     NIGDTNIEELLKFAKENEIGLTIVGPEVPLVMGIVDEFEKEGLRVFGPNKKCAQLEGS
                     KAFSKEFMIKHNIPTAKYKEYTNLEEAISEIDSFGYPVVIKADGLAAGKGVVIPENRE
                     DAIATLKEMMSDKKFGAAGDKIVIEEFLKGIETSILAFVDNDTIVPMASAKDHKKVNN
                     YEQGPNTGGMGTFSPSEIYTEELANKVKETVLEKTLEGFKKDGLNFKGILFVGLMITE
                     DGEKVLEYNVRFGDPETQSVLFRLETDLHEIMETILDNKLKDIEINYSDDEAVCVMLT
                     SGGYPDSYEKGKIITGLENLDDDIVVFHSGTKMLDGNLVTNGGRVIGITAKSTTVKDA
                     AEKVYENIKKINFEGMHYRTDIGR"
     gene            35299..39105
                     /locus_tag="PC700_000259"
     CDS             35299..39105
                     /locus_tag="PC700_000259"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HDF2579587.1"
                     /translation="MLNTENKDSMVRRVLVEKREGFDLEAKALKKDLVESLHIDNIEN
                     LRILNRYDVEGISEEVYENAAKTIFSEPNLDVVYYEEIPKLNDERVFAIEFLPGQYDQ
                     RGDWAAQCVQIVNQGIRPAINTAKVYILSGKITDEEFSKIKDYCINPVDSREASLEKP
                     ETLKMETEIPTTVEVLDGFIDLDENGLRTFVSEKGLAMTLGDLQHVQKYFKDTEKRNP
                     TITEIKVLDTYWSDHCRHTTFMTEIENVKIEDGKFNDIVKEAYQMYLNSRDNVYVNRH
                     KDICLMDIATVAVKELKKNGKLNDLDESEEINACSINVDVEVDGKMEKYLVMFKNETH
                     NHPTEIEPFGGAATCLGGAIRDPLSGRSYVYQAMRVTGSADPRTTLEDTLPGKLMQRK
                     ITTEAAHGYSSYGNQIGLTTGQVAEVYDENFVAKRMEIGAVIAAVPKENVVRERPEAG
                     DVIVLLGGKTGRDGCGGATGSSKEHSEESILTCSAEVQKGDAPNERKIQRFFRNKEVA
                     QMIKRCNDFGAGGVCVAIGEIADSLDINLDLVPKKYDGLDGTELAISESQERMAVAIK
                     KENKDKFIQLAVEENLEATHVATVTDTGYLRMFWNGKAIVDINREFLDTNGVKQTTDV
                     HVTKVDEENTFFSSNEIVKDVKCASMKDKFTKVLSDLNVCSQKGLVEMFDNTIGGNTV
                     LMPFGGKYQATPTQGMVAKIPVLGGETNTSTIMTYGYNPKVGKWSPFHGALYAVVESV
                     CKLVAIGGNYSTTRLTFQEYFEKLGNNPEKWGKPFSALLGAFYAQSKFEIPAIGGKDS
                     MSGTFKDIEVPPTLVSFAVDTVDAKKVVSPEFKKADSKVVMLCVNKAENDVVDFEELK
                     RNLDKVRELIHGNKVLSTYALGFAGVGEAISKMAFGNKIGFKFSEEAEKTFTDDKLFE
                     ASYGNIVLELANDDLCMLEGYNYVVLGSTTKEASIFIKGEELALDELYKAHCSTLEPI
                     FPTKTEEVKSKIETISYISQGEAKKSSLSIATPRVFIPAFPGTNCEYDSARAFERVGA
                     NANIRVFKNLTYKDIEDSIDTIVNEIKSSQIIMLPGGFSAGDEPDGSGKFIATVFRNP
                     RVQEAINEFLTQKDGLMLGICNGFQVLIKLGLVPYGEIRVPSESAPTLTYNNIGRHQA
                     KIARTRISSNKSPWLAQTNVGDIHNIAISHGEGKFVASEDVMRELIANGQVATQYVDF
                     NNEATYDIEFNPNGSFYAVEGITSADGRVFGKMGHSERIGEEVFKNIIGEKDQKIFES
                     GVKYFR"
     gene            39311..40183
                     /gene="rfbD"
                     /locus_tag="PC700_000260"
     CDS             39311..40183
                     /gene="rfbD"
                     /locus_tag="PC700_000260"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HDF2579588.1"
                     /translation="MKILITGSNGQLGKELVSQLEAINQSINQPKYVILATTRNDLDI
                     SNQTNIDNFILHNKPDVIVNCAAYTKVDACEDNIEIAYKINALGVRNLAIASEKVNAK
                     LVHISTDYVFNGFSKYPYREDNKTEPNSVYGKSKLMGEKFVEQFSHKYFILRTAWLYG
                     NGNNFVKTMIKLSLENKEVNVVNDQFGTPTSTVDLAKIIIRIMETEHYGVYHATCEGE
                     CSWYDFAKKIFELKNIDIKVNPIKSNEFKCKAQRPLYSVLDNFMLKLIGLNSFRKWEE
                     SIEEYLERYDINEK"
     gene            40292..41173
                     /gene="rfbA"
                     /locus_tag="PC700_000261"
     CDS             40292..41173
                     /gene="rfbA"
                     /locus_tag="PC700_000261"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDF2579589.1"
                     /translation="MKGIILAGGSGTRLYPITKCISKQTLPIYDKPMIYYPMSVLMLA
                     GIQDILIISTPRDIKAFEELFGDGSSLGLKITYAIQEKANGIAEAFIIGEEFIGNDRV
                     SLVLGDNIFYGYGFSERLENAVKRKGATIFGYHVSNPNSFGVVEFDKDFNVLSIEEKP
                     TVPKSNYAIPGLYFYDNDVVEIAKLVEPSDRGELEITSINSEYLKRGKLKVELLGRGM
                     AWLDTGTHKGLLDASNYVEVVQTRQGLYIACLEEIAYTKGYISREQLMKLAKPLIKTE
                     YGQYLLKLASEDMQYGR"
     gene            41210..41767
                     /gene="rfbC"
                     /locus_tag="PC700_000262"
     CDS             41210..41767
                     /gene="rfbC"
                     /locus_tag="PC700_000262"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HDF2579590.1"
                     /translation="MGKINVIKTKIEGLYIVEPNVFCDERGYFMESYNKKDFIEQGLD
                     MCFIQDNESSSRKGVLRGMHFQVRYPQGKLVRVTEGEVFDVAVDLRKDSQTFGKWEGV
                     ILSDKNKKQFYIPEGFAHGFLVLSEKAVFNYKCTNYYYPEFEHGFIWNDKQISIEWPL
                     KGIEEIFLSEKDKKQKSFKDICIRR"
     gene            41805..42788
                     /gene="rfbB"
                     /locus_tag="PC700_000263"
     CDS             41805..42788
                     /gene="rfbB"
                     /locus_tag="PC700_000263"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDF2579591.1"
                     /translation="MKKILITGGAGFIGSNFVHYMLNKYVDYFIVNLDLLTYAGNLET
                     LIEIQDSSNYKFVKGDISNREQMYKLFEEERFDIVVNFAAESHVDRSVENPDLFIKTN
                     IIGTQVLLDASIKYGVKRYHQISTDEVYGDLPIDRKDLFFTEQSPINPSSPYSASKAS
                     ADLLVSSYYRTYGLLTTISRCSNNYGPYHFPEKLIPLMILNALENKQLPVYGNGENVR
                     DWLHVYDHCTAIDLIIHKGNIGEIYNIGGHNERSNLEVVKMILNLLDKSEELISYVND
                     RPGHDLRYAIDATKIENELGWKAKYDFDLGIKETVKWYIENESWWKAVLSV"
     gene            42827..43495
                     /locus_tag="PC700_000264"
     CDS             42827..43495
                     /locus_tag="PC700_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HDF2579592.1"
                     /translation="MNEISSLINILALTQLSGSNTTNQCNCTYNSSSGFDMIMMTLLK
                     ALSQNNQQTSNGYNLSSNSENLFDELGSVVNNTASRFIDVDTKDKSVKSRIENAIEQA
                     SKKYNVDSNLIKAIIKVESDFNPNTVSSAGAKGLMQLMPENCRDLGVTDPFNIEQNID
                     AGTRHIKEYIDMFGGSIEMGLMAYNGGPGRMKSRGVESISDLYKMPKETQNYIPKVMK
                     YYRG"
     gene            43504..43932
                     /locus_tag="PC700_000265"
     CDS             43504..43932
                     /locus_tag="PC700_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HDF2579593.1"
                     /translation="MGINIDKLNISPSINMEVKKLKESNKFSDKLNSLKQSSSLEDIK
                     RNLESVKKAGERLVLTQNYGDISIYKNAVKEYLKSVVDNMYSLDKNSSFWEHQYYKNV
                     KIVDSKLEDMTSKLISEEKENIDIVSTVDMIQGLLIDMYR"
     gene            43956..44270
                     /locus_tag="PC700_000266"
     CDS             43956..44270
                     /locus_tag="PC700_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HDF2579594.1"
                     /translation="MNSKKEDIYELNFGQLIEEKSGVIEENQNVFNAKVNVAVSIGNT
                     TETIKNILDLKEGSIIKLSKNVDEKLDIYANDRLFAYGESIITNEKLSVRLSKIEDSD
                     VN"
     gene            44422..44697
                     /gene="flgM"
                     /locus_tag="PC700_000267"
     CDS             44422..44697
                     /gene="flgM"
                     /locus_tag="PC700_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HDF2579595.1"
                     /translation="MDIKNVSSNIVVNNYLKNTSGVKKVEEVKKINKTDKIELSKFAK
                     EMASVSNMSDENIRNKKVDEIKNALENGTYKIKPKEIAKKMIEDMRG"
     gene            44702..45118
                     /locus_tag="PC700_000268"
     CDS             44702..45118
                     /locus_tag="PC700_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HDF2579596.1"
                     /translation="MITDLKVIIYEEKNIISKMMELLKEQFDYIVEKDLENLNMLNPK
                     LEDISRELASIEIKRRQLFGDDISMSEVVENSNDRYLKEIYTDLKHILALAKNQQESN
                     DSLIKKELIFTKKMINFIKPADKQTATYNSYGNIRK"
     gene            45136..46446
                     /gene="flgK"
                     /locus_tag="PC700_000269"
     CDS             45136..46446
                     /gene="flgK"
                     /locus_tag="PC700_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HDF2579597.1"
                     /translation="MAGLFSILNTANSGMNAHQKAIQTISHNISNLDTDGYSRQRTEF
                     ATNSPMYMPSLSNSIARGQLGTGVHVTDVTRARNSFYDYQFRAEAHKYGNTVSKYDYY
                     NTIETIINEPSDYGISAGIDDFFKGWESLAKDINSDSKKSLVVENASNLATLISESYK
                     KLNKLKGDIDDNINSEIKQINDMLKSLEDLNKSIDVISGSGSTPNDLLDERDRILDDL
                     SFKLDLENSDVKNMLSDGKLELNELKNADGTWKTGVSGTLQGLFEMHGKIDTYKSDLK
                     DVSDGLAKQINDVYNTSAGITVTDFFITSNVDGEDIIKVNPAIKSNPNELKLTADEAS
                     KIAKLKDEKIDIGVAGGKVSTISDHYKAFAESVGLDSQKVNQDEVNQRKIINNIDNSR
                     MSVSGVSLDEEMTELIKIQRSYQASAKVMSTAVQLLDVVINGII"
     gene            46465..47397
                     /gene="flgL"
                     /locus_tag="PC700_000270"
     CDS             46465..47397
                     /gene="flgL"
                     /locus_tag="PC700_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HDF2579598.1"
                     /translation="MRVSTGMMSSNYLNSLQDNLQRLDKVNRQVNTTKEINKLSDNPY
                     KAIKILNSKSEIKTMETYVENCKDTGNWLETTDTALDQLGKLLSDIKKTLVASGDGAY
                     SDDELKTLSNETNEKMKEIANALNATHEGKYIFSGSNTGVPPVECIDNADGSVSLKFN
                     SSLNLNKLNDSLSMDIAQGISVDYNVKLSSLGFDPTKTDPFEELNNISKKLANPDADA
                     IKELTTTCLGDMEQLIENTVNVRSIYGTKANTVEAMKEKNDDSLIQLKDILSQNEEID
                     YGEKLVQLKAAELTYQASLQTGGKLFNVSILDYI"
     gene            47413..47805
                     /locus_tag="PC700_000271"
     CDS             47413..47805
                     /locus_tag="PC700_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HDF2579599.1"
                     /translation="MMKVTLKKGILGFENLKEYELLDIEDNDSLKEFNSTEEEGIGFV
                     VVSPFEIINEYEIVLNQETIEKLEVKSPKDVVLFNIITLGQTFEESTVNMKAPIVINI
                     KTNFGMQIILQDDKYSIRHPLLRGDNGC"
     gene            47799..48011
                     /gene="csrA"
                     /locus_tag="PC700_000272"
     CDS             47799..48011
                     /gene="csrA"
                     /locus_tag="PC700_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HDF2579600.1"
                     /translation="MLVISRKKDEAVLIGESIEVKIVGVDGNNVKLAISAPNNISILR
                     KEIYEKVKSENIKATNKNIKILKSLK"
     gene            48033..48428
                     /gene="fliS"
                     /locus_tag="PC700_000273"
     CDS             48033..48428
                     /gene="fliS"
                     /locus_tag="PC700_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HDF2579601.1"
                     /translation="MYGENPYNSYKQNAVFMASKEQLLLMLVDGAVKYTKIARAAILD
                     KNTQKAHRELVRVQDIFTELMVTLDQNAGQWAKDMYKVYDFVRYELSRANIRKDIQVI
                     DNVLPVIEQIKDTWHEADKKSKEERSRYK"
     gene            48428..48784
                     /gene="fliS"
                     /locus_tag="PC700_000274"
     CDS             48428..48784
                     /gene="fliS"
                     /locus_tag="PC700_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HDF2579602.1"
                     /translation="MELNLAELDKLSKEELLLMLVDGTVKYTNISKEALLNNDYLKAH
                     NELIRVQNIFTELMTTLDQDAGQWAKDMYKVYEFIKSELAIADENKDIKIIDDILPIV
                     KQIRDTWHEVYNQLKI"
     gene            48805..50328
                     /gene="fliD"
                     /locus_tag="PC700_000275"
     CDS             48805..50328
                     /gene="fliD"
                     /locus_tag="PC700_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HDF2579603.1"
                     /translation="MSSISPIRVTGLSGNFDMEGIIEASMIRDKEKVDKAKQEQQIVK
                     WKQEIYRNVIQESKDLYDKYLSVNSPNSIVSEKAYSSTRITSSDESIIVAKGSAGAEK
                     INYQFAVSQMAEPAKFTIKLNSSEPIVRQFPPNASGASSLTIGDVNIPISEQDTTSTI
                     VSKINSLCADNDIKASYSEMTGELIISRKQTGSSSDINLKVIGNDNLAQQIANDNGIT
                     FANDASGNKVASVYGKNLEADVTDEHGRVTHISKEQNSFNIDNIDYNVNSKGTAKLTS
                     VTDTEEAVKNMQAFVDDYNKLMDKVYGLVTTKKPKDYPPLTDAQKEDMTTEEIEKWEK
                     KAKEGILRNDDELRGFVEDIQSAFFGDGKNIIALRKLGINESENYNKKGQISFNADTF
                     SKALIDDSDKVYKTLAGYSSNYDDKGMFEKLKDIVYEYSGSSTSKLPKKAGIEKTASA
                     SENVYSKQIAEQERNISRLVEKMNDKEKRLYAKYSALESLLNQYSSQMNYFSQAQGN"
     gene            50331..50666
                     /gene="fliT"
                     /locus_tag="PC700_000276"
     CDS             50331..50666
                     /gene="fliT"
                     /locus_tag="PC700_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HDF2579604.1"
                     /translation="MDKLKEKLNLYKDISLQIINLIEKEEYIKISSKLGERQEIINSV
                     SEIDRNDFIQLYNRMELIEIDSRIRDILQGQLLEVKKELHEYKLTKQVNTMYYNLNRE
                     KVNIFNKKV"
     gene            50768..51640
                     /locus_tag="PC700_000277"
     CDS             50768..51640
                     /locus_tag="PC700_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HDF2579605.1"
                     /translation="MRVNTNVSALIANNQMGRNVNAQSKSMEKLSSGVRIKRAADDAA
                     GLAISEKMRAQIKGLDQAGRNVQDGISVVQTAEGALEETGNILQRMRTLSVQSSNETN
                     TAEERQKIADELLQLKDEVERISSSIEFNGKKLLDGSSTEIRLQVGANFGTNVAGTTN
                     NNNEIKVALVNTSSIMSKAGITSSTIASLNADGTSGTDAAKQMVSSLDVALKELNTSR
                     AKLGAQQNRLESTQNNLNNTIENVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLA
                     QANQQPQGVLQLLG"
     gene            51733..53874
                     /locus_tag="PC700_000278"
     CDS             51733..53874
                     /locus_tag="PC700_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF2579606.1"
                     /translation="MLLSIVMIVKNEEKILEKTLKSLTTLRSSIESELIIVDTGSTDD
                     TINISRKYTENVYFHNWNNDFSSMRNISISYAKGEWLLILDADEILIDSSTIVKFFKD
                     GLDKKFNSASIQLKNLYSYDKKLYGYCSVLRLFKNIDFRYHGKVHEQPIYKNPIFNNV
                     ADFEHYGYLFEDEEIRLNKVKRNEKLLFEELKENDNSPYTNYQLGKNFIILGKYHEAL
                     YYLEKSYELYSKLESAPGYLITSLVKTYLYLGKHKKCEKLCLKYIKKDMNNIDIYYYI
                     AQAQVDLGKYENSIDSYKRYIYLLDNYEISTQANSLFSDTDTVGLRDEAIITLIKIYY
                     KLEKYESVISEYNNIEDMEKKKNVYFSLFMSLYKLNRFEDIKNYYKEVSNSKIEKNSF
                     YKNIEEVINNIKDDEKEHVYKILCDIEGNYGILNQIRINKCVSIDKCKEILNEEKEII
                     YAYIIKVAFEDKIDLLDIFYEMEYVWIEKYLKYLLALERSFNLVLYKYIISRPNTSDI
                     KKIRVYKAISKVVLESISLSDEKYKEIFYLYIMYSYKYIRYIYRNLSDFELIKFVCND
                     LDKFTIEFKHLLDIKNKVNDANEKLEYIHSMRKLLNEYPFYNKIIKILISELEENIKE
                     SEEFKDLKDSFIISIENMIETGKINDAKSLVDDYSKTFMDDVKILNIKGILYMFENKL
                     DKADFMLKKALSLDVENEDTIYNIEYLKSLR"
     gene            53898..55775
                     /locus_tag="PC700_000279"
     CDS             53898..55775
                     /locus_tag="PC700_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF2579607.1"
                     /translation="MNTPIVVNEKSKDIIKDWQYTYTNEDIKFTSIIILTYNQIEYTK
                     LCIESIRKFTPKNKYEIIIIDNNSSDGTVEWLKGQSDIKSVYNNKNLGFPKGCNQGIE
                     LATGENILLLNNDVIVTPNWLYNLNKALWSQDNIGAVGPITNACSYYQQIDVDYKSIE
                     DMFMFAHKINTSNINLWKYRLKLVGFCMLIKKSVLDNVGVLDELFTPGNFEDDDLSFR
                     MLESGYKLLLCQDTFIHHFGSVSFKENRNKYTDIMYINRRKFETKWGFNPEYSGMIRT
                     DLISKIDDSLEANINVLEIGCGIGATLLEIKNIYKNASIYGIEISESAGKLAKYSADV
                     LIADIEKVKLDYEKDFFDYIILGDVLEHLTDPWKIVNELKQYLKKDGCLISSIPNIMH
                     VSVLRNLVLGNFTYEDSGILDKTHLRFFTLNEITKMFNDSNYIINSVTATSMPLSEID
                     NRFIDTMCKESCKEMRQQYESYQYIVKATKSIDTKRYSSISMVNLKYALMRIDNELDV
                     DSNLSYIFDNYNITEPCFVDDIEYLVDTFVINKATLLNRLGVEAFNRGLGDFSINMFM
                     TVLEMDRDDIDSIYNITSVLCELEEFDTAYNLIKNSSKKVQSESDIQDILYFIEGKRY
                     E"
     gene            55768..57882
                     /locus_tag="PC700_000280"
     CDS             55768..57882
                     /locus_tag="PC700_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDF2579608.1"
                     /translation="MNNDKICFITCVNDERLYEECLVYINSLDIPKNFSIETVALRGA
                     DSIASAYNKAMRETDSKYKVYLHQDAFIINKNFIYDILEIFKKNTDVGMIGVAGVKDI
                     PVNGIWWDSDKKAGKVYDSHTGKMDIYEFSNFEECFNEVMAIDGLIMITQYDVFWRED
                     KFDGWHFYDLSQSMEFRQNKYKVIVPKQNKPWCMHDSGVANINNGYDEYRIKFVKEYI
                     NLDLYRENFYEFDKKKASTILKSNKHVMDCMKEELTEQLRLKNYKDVLDIVSKIAHFS
                     VFNHTGLYSCEKCERALLECANNLPEIENTKLFTKKDLKRRKVLHVLSEGYSTGGHTR
                     LVKNWIEIDKDSVHSVVTTWQSDTLPDWLLKAIDDSGGEVISLDLISDDYLKRAVALR
                     KIAYDWADIVILYVHMWDTIPVVAFGVDGKIPILHVNHYDSTFWLGTSIADIVIDFSE
                     ASQLITLDKRGIDKSEILPMPLKEKEVFSKSEVRKRYNLEDDVTIILTIASERKYNSV
                     NDINYFKIAKTLIENTDNTIIIVVGVDTENLYWKTLSNVTDNRVICFGTTANIDDFYK
                     ISDIYLESFMVGSHTSKLDAILYDLIPVKFKNEVSPIFTNMWNFIDKFDYKNIKEVID
                     MIRRYNTSEFDRDKIKEKQSEIKHFVIDKHCIEIKNYIDKIYKEANHHKVKYDLYKND
                     EIEDYQLFVALYGYMVKNKNMS"
     gene            57891..58886
                     /locus_tag="PC700_000281"
     CDS             57891..58886
                     /locus_tag="PC700_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HDF2579609.1"
                     /translation="MNNIHINKFEKSCEKNWDYFVENKSINGTFLQTRNFLNYHEEGK
                     FTDHSLIIYKGEEILAVVPACETYENGERVFFSHLGSTFGGIIIRSGFNDIRHVNAIT
                     NKLETYLQEQSFNKVYLKNTSRIFCKEDTSLLEYFLFKNNYMFYDELSMFLELSKYKK
                     DILANFRYSKRRYFKYSLKNNLTFKRLYSDEDIVCFFNLLSNCLDSKGAKVIHSVEEL
                     LEFKNNRLKDIVEFYAVYIDDIMICGSMVFNFQERVYHTQYLASNSTYNKYYPMIFLY
                     YNLIKDALERNFKYLSFGISTLNRGKYLDEGLATFKEGFGSSAICNRTYYKNINK"
     gene            58978..60210
                     /locus_tag="PC700_000282"
     CDS             58978..60210
                     /locus_tag="PC700_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HDF2579610.1"
                     /translation="MKRLMILGSIKYFENIVMSAKKEGIYTIVCDNRIDTPAKKICDE
                     AIDVDVFDFDKIKSIAIDKKIDGILTGFTDSLMKPYVYIANELNLPCVISCNQLESVT
                     NKAIMKKYFKNNAIPTTDYCIIESLKDLEKIQGLNFPLVIKPVDGYGSRGVYFVSNIE
                     ELIEKFEYSKEASQDGQVIIEEFYESEEIQGLAWVHNGESHVFYIGDRELVNIHTGRA
                     GKPDRLLYPSKYCFQYEEEIKSIYQNITQAFDIKNGPLYVQMLVGAYGIKVSEVMPRL
                     PGGCDYMAISQITDLDIGSLFANFSVNNEVNFDEIKKYNMHLSKCVYALPIYIRPGTI
                     KKINNIDKIENLDYVTQFLLLVKEGDTIGLYEDERQDCGRVYGIANNIFDANIKKKCI
                     HEMIEIFDENNENMIENF"
     gene            60230..61174
                     /locus_tag="PC700_000283"
     CDS             60230..61174
                     /locus_tag="PC700_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HDF2579611.1"
                     /translation="MKLITHEQIMSLNISQKQGYEWIKESFLTKKDCQLPPKISMVEE
                     GHVFYNVMPSLNHNHMIGGIKMVTRLPGRNPSLNSDIFLYNMKDAKLKAILDGNYITA
                     FRTAAVAGLAIDTFAKKDFNTISIMGLGVTASAIMNMLSYTLKDKNINIKLLKYKDQA
                     ESFIKKYEKNEQFSFEICDTHEELIVDSDVVISTVTYTDEYLSEFKWFKKGALLLPVH
                     VMGFGNVDYLFDKVYLDDYNHLKHLENIDRYKYSCEFYNVLSKQAKGRENDDERIISY
                     NIGLSIHDILFANKIYELCETLNCEEIELKEPKEKYWI"
     gene            61268..61675
                     /locus_tag="PC700_000284"
     CDS             61268..61675
                     /locus_tag="PC700_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdtA/QdtA family cupin domain-containing
                     protein"
                     /protein_id="HDF2579612.1"
                     /translation="MDKYNLIKFLSIGNEYIGNLVALESNKAIPFDIKRVYYIYDVPN
                     NVKRGFHAHKNLEQVLVCLNGSVKIKCYDGVKESIFNLHKNDVGLYIGTGVWHEMFDF
                     DEKTVLISIASEHYNEEDYIRDYKEFLKYLNNM"
     gene            61762..62853
                     /locus_tag="PC700_000285"
     CDS             61762..62853
                     /locus_tag="PC700_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HDF2579613.1"
                     /translation="MLVKFNNLLKQFQTHKEEYEQKIKDVMESGYYILGNEVRHFEEE
                     FASYVGSKYCVGLASGLDALIIAFRALGISEGDEVIVQSNTYIASVMGISINGATPVF
                     VEPNEYYNIDSSKIEEKINSKTKAILVVHLYGQASKMKDIQELCDKYNLYLIEDCAQS
                     HGAKYDGKMTGTFGDIGCFSFYPSKNLGAFGDAGAIVTSNEEIADKVKILRNYGSEKR
                     YYNKFVGYNSRLDEIQAGLLRVKLKYLNEIEIEKSKIVDRYLNEINNPKVILPKIENN
                     CTHVWHLFVLQVEKRDTFMEYLKKNGIESIIHYPIPPHLSEAYAYLGYKKGSFNIAEN
                     YSDKVVSIPLFVGMEDEEVSYIIKIINEY"
     regulatory      63162..63252
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT        25083 a         6679 c        11347 g        20341 t
ORIGIN      
        1 ataaattttg aaattactta tctttgtgtt aaaataagta aaatacacat attaaagtta
       61 agtttttagg caaataaaca aaatgaaaat ataaatatta aggagatttg gtatatgaat
      121 gtttatgaaa aaggtaatgg agttatacta gaaggagtaa cagattttga cccagtacat
      181 atatttgagt gtggtcaatg ttttagatgg cacaaacaag aagatggctc atacactgga
      241 gttgcaaggg ggagaatatt aaatgtcaaa aaagagaatg ataaaatata tttaaataat
      301 acgaatttaa aggagtttaa cagcatttgg tataattact ttgatttagg aactgattat
      361 acagaaataa agaataagct aaaaaatatg gatgaatatt tgaataaggc tactgaattt
      421 ggatggggaa taagaatatt aagacaagat ggatgggaaa tgcttatatc atttataatt
      481 tcctctaata atagaattcc tatgatacaa agagcgatag aaaatttatc aagaaagttt
      541 ggtaagtata taggtgaata tgaaggtaat gaatactatg cattcccaac accagaagaa
      601 ctaaataagg cctcacaaga ggaaataaga gcatgtcaaa caggttttag agataagtac
      661 ataaaaagta ctacacaggc agttatagag aataatgatg aggtatctga atatacaaat
      721 ttaagtacag aggattgtag aaaagagctt ttaaaattta atggagtagg accaaaagta
      781 tgtgattgta ttgcattatt tggtatgcaa aaatatgatt cttttccagt tgatgtttgg
      841 gttaagagag ttatgcaaga attttatata gatgaagaca tgagtttacc taaaatgaga
      901 acttatggaa tcgataaatt taaagaaatg tctggatttg ctcaacagta cttattttac
      961 tatgcaagag aactcggtat aggaaaataa attaaggggg agaaattatg ggagtaatag
     1021 gaacaatatt tctgttctat ttaattatat catatctagc tggagccata atttctgtga
     1081 ttatattgct tgaaaataga gaccctgcaa aaacaatgtc ttggctattg atgtttatta
     1141 tatttccagg tgtaggttta atgatatatg ctatttcagg tagaaatatt agaaaaagaa
     1201 aattatttaa gactcaaaaa ctagctaata atataaaaga aaagaaacta tttgatacat
     1261 tagaaaagat aacagaaata gttgaattag aaaaagaatc tataaaacaa aataagcttt
     1321 taagagatga agaggatggt agttatagaa aaagggtaat aaatatgttg ttaaagactg
     1381 gtatgtttcc ctttactaaa aataataaag tggatgtatt tgtagatgga aatgaaaagt
     1441 ttaaaagact tatagaagat ataagagaag ctaaagacca tattcattta gagtacttta
     1501 tcatcaaaga tagtgaaata ggtagggtgt taaaagaaga acttataaaa aaggctaaag
     1561 aaggcattaa gataagaatt ttatatgatg atgtaggatg ctggagattt tggtttaata
     1621 gaaaattttt ccgagaaatg agggaagtag gtatagaaat tgcagcattt ttacctacaa
     1681 aatttcctat aataggaggg aaattaaact atagaaatca cagaaaaatt gttgttattg
     1741 atggaataat tggatataca ggtggaataa atataggaga tgaatattta ggcaaaaatg
     1801 ataagtttgg atattggaga gatactcata ttaggattaa gggtatttct gtatacatgc
     1861 ttcaaatgac atttctgatt gattggtatt atacgacaaa agaagtatta gtgactaaaa
     1921 attatttccc aagtgtaggg aatgttggtg aaagtatgat acaagttgtt gcgagtggtc
     1981 cagacagtga ctgggaagat attcattatg cttatttttc tgcaatatgt caggctagaa
     2041 aaaatgttta tatagaaact ccatatttta ttccagatga aagtctacta aaagctataa
     2101 agagtgctgc tcttagtgga gttgatgtga gaataatatt tcctaaaatt gcagaccaca
     2161 agatagtaaa tatagcatct tattcatatt ttgaagaaat attaagagca ggtggtaaag
     2221 tttatttata taataaagga tttatacatt ctaaagtcgt aataatagat gataaaattg
     2281 cttctgcagg tacagctaat atggatttaa gaagttttat gcttaacttt gaggtaaatg
     2341 catttatcta tgatgaagaa gttattaggg taatgacaga tgatttcttt gaggatttaa
     2401 gtcactgtga agaacttaat ttggaggtat ttaagaatag aaatatcata caaaaaatca
     2461 aagaatctgt agcaagatta ttttcaccaa tattataaaa aattattaaa aaaggtattg
     2521 aaaattatgt gtgattggta tattataata attgttagca ctctattaga tagagtgata
     2581 ataaataaaa agtatatata taaatctaca tatataagta gtattaattt aaaaataact
     2641 aaaaacatag gcgaactagt ctatacttaa tattttagga ggcgtgaata atgaaaataa
     2701 gaccattagc tgacagagta gtaattaaaa aagtagaagc agaagaaaaa actgcaagtg
     2761 gaatagtttt accaggagca gctaaagagc aacctcaaat agctgaagtt gtagaagttg
     2821 gaccaggtgg aatagttgaa gggaaagaaa taaaaatgga attgacagtg ggagataagg
     2881 ttatattcca aaaatattct ggaacagaag ttaagataga aggacaagaa tacacaatac
     2941 taagacagag tgatgtatta gctgttattg aataaatata gaataaattt attaggaggg
     3001 atttaaaatg gctaaagaaa ttaaattttc agaggaaaca agaagagctt tagaagctgg
     3061 tgtaaataaa ttagcagata cagtaaaagt aacattagga cctaaaggaa gaaatgttat
     3121 attagataaa aaatttggtt ctccacttat aactaatgat ggagtaacta tagcaaaaga
     3181 gatagaacta gaagatagat ttgaaaatat gggtgctcaa ttagttaaag aagtagcaac
     3241 taaaactaat gatgtagctg gagatggtac tacaactgct acagttttag cacaagctat
     3301 aataagagaa ggtttaaaga atgtaacagc aggggctaac ccaatacttt taagaaaagg
     3361 aatacaaaaa gcagtaatag tagcggtaga agaattaaaa aatcaatcaa gaatagtaga
     3421 aacacaagag gctatatctc aagttgcttc tatatctgct ggtgatgaag aagttggaaa
     3481 attaatagca gaagctatgg aaatagtagg taaagatgga gttataactg ttgaagagtc
     3541 tcaaactatg aatactgaat tagatgctgt tgaaggtatg cagtttgata gaggatttgt
     3601 ttctgcatat atggttacag atgtagataa aatggaagca gttttaaatg acccatatat
     3661 attaattact gataagaaaa tatctaacat acaagaatta ttaccagttc ttgaacaaat
     3721 agttcaacaa ggtaaaaagt tattgataat agctgaagat gtagaaggtg aagcattatc
     3781 tacattagta gtaaataaat taagaggaac atttgatgta gtagcagtta aagctccagg
     3841 ctttggagat agaagaaaag aaatgcttca agatatagca atacttacag gtgctcaagt
     3901 aatatcagaa gaattaggtt atgatttaaa agaagctgat ttatctatgc taggtagagc
     3961 ttcatctgtt aaggtaacta aagaaagtac tacaatagta gatggttctg gagataaaaa
     4021 agctatagaa gatagagtaa ctcaaattaa acatcaagta gagcaaacta cttcagattt
     4081 tgatagagag aaattaatgg aaagattagc taaacttgct ggaggagtag ctgttgtaaa
     4141 agttggagct gctacagaag tagagttaaa agaaagaaaa ttaagaatag aagatgctct
     4201 taatgcaact agagcagctg tagaagaagg aatagtagct ggtggaggaa ctgcttttgt
     4261 tagtgttata ccagcaatag gaacactaat tgagagttta gaaggagaag ttaaactagg
     4321 tgctcaaata gttaaaaaag cgttagaaga gccattaaga caaatagcta taaatgctgg
     4381 tcttgaaggt gctgtaattg tgcaaaatgt tgttaattct gaagcagaaa ctggatttga
     4441 cgcattgaat gaaaaatatg taaatatgat agaagctggt atagttgacc caactaaagt
     4501 tagtagaagt gctttacaaa atgctgcatc aatagcaagt actttcttaa ctactgaagc
     4561 tgctgttgct gaccttcctg aaaaagaaga tgcaggaatg ccaggaatgg gtggcggaat
     4621 gcctggtatg atgtaattta aaaaatttaa aatataaatt ttaatataaa gaactatttc
     4681 gacttagatt ttaaactcta agttgaaata gttcttttgt atatatctaa tttttgtata
     4741 tctataaatt caatacacat atttagataa tcagtatcta gtttgtaata atattttaga
     4801 gtagtattgt tattttttag gttatgattt tagatatata ttaattaagg tataattgtt
     4861 gatagagaat aaatcaaatt taaaaggatg cgataatatg gacttaagaa aattacttga
     4921 agaagttaaa aatgaaaata ttgatataga ttttgcgtta gaaaagttaa aagatttacc
     4981 ttatgaagac cttggatatg caaatataga ccatcataga gaattaagaa atgggtatcc
     5041 ggaagttatt tattgtgaag gtaagagtga tgagcatatt ttagggatta ttgataaaat
     5101 gaatcaaaaa agttctaaca tattggggac aaggtgcaga aaagaaactt ttctgaaaat
     5161 taaagaaatc tataatcatg ctgagtacga agaggcgtct aaaattctta agattcaaaa
     5221 tcatgatata gagaatatag gaaaaggtaa aattgtgatt gctacagggg gaacttctga
     5281 tataccagta gcagacgaag cttatcatac agctaagttt ttaggtaatg atgttgatag
     5341 aatatatgat gttggagtgg caggtataca taggctttta aataaaagac ataaaataga
     5401 tagtgctaga gtaatagtag ccgttgcagg tatggaaggt gctcttgcaa gtgtaattgg
     5461 tggactggtt gatgttccag taatagctgt tccaacttct gttggatatg gagcaaattt
     5521 tggaggatta gcagcattac tagctatgtt aaatagctgt gcatctggaa tatctgttgt
     5581 aaatattgat aatggatttg gagcaggata tttagcatca atgataaata aactgtaaat
     5641 acattgatat aaattaaaca aaactatata ataaaattta atttctatta tatttttact
     5701 attaaaattg aggagacata tatgagtgaa aaaatacttt attttgatat aataagcggt
     5761 atatcagggg atatgaccct tgcaagttta ttaaatttag gtgtgcctaa ggaaattttt
     5821 ttggaggaaa ttcataaact aagaatgagc gatgaattta atataaacat cagctcgaaa
     5881 actgaaaatg gaatagtagg tacaaaagta gaagtaatta caaaagaaaa acatacacat
     5941 agaaatttag tggatatatt tgaggttata gataaaagca ctctaaacga aaatgtaaaa
     6001 tctaaagcaa aaaaaatatt tatggtaatt ggagaagcag aagcaaaagt tcatggaact
     6061 acaatagaca agatacattt tcatgaggta ggggctatag attcaattgt agatatagta
     6121 ggagcttcta tattagttga cttactttgt gtagataaag tttgtgctag tacagtgtct
     6181 gttggttcag gatttgtaaa atgtgaacat ggaattatac ctatacctgc acctgcaact
     6241 atagaaattt taaaaggtgt gccaattaaa ttaaataatg taaatggaga atgtacaact
     6301 ccaacaggag ctgcaatcat aaaagtcttg tgtgatgagt ttgtagatga gcttgaattt
     6361 tctccaaagc aaattggata tggtataggt cataagaagt ttgaagtacc aaatatactc
     6421 agaactgtat taggtgaaaa aaaaaagaag aattaattta tgaaattagt gcaaacattg
     6481 atgatatgtc ttcagaaatc tattcttatt tgtatgaaaa aatattagat gagaaagcat
     6541 tggatattta tacagagagt atttatatga agaagaatag accagcaaca aaagtatcaa
     6601 ttttatgtaa tgaatcagat ttagaaaagt ttattaaaat attactttta gaaactagta
     6661 cttttggagt gagatataat gcatataata gagaaatttt agagagaaaa tttgtaaaat
     6721 tagatacaaa atatggagtg gttacagtaa aactagggta ttataaagga aaactagtca
     6781 aggttactcc agaatataat gaatgtaaaa ctatagcaaa aaatgaagat attcctataa
     6841 aaaaagtgtt tgctgatata aattttagaa taaaagaaga atttagtgaa aacttattga
     6901 cataagtaat aaattttgat aaattaatat tcagccttat taaataaaag taaacagaaa
     6961 aaaatatata aaatataaac tcactcgtat atgctcagaa atatggtctg agagtctcta
     7021 ccaagatacc gtaaatatct tgactatgag tgaaattatt ataccaaaag acctagttaa
     7081 gtaaatgtgt aggctgagtt atactcccta tgtgttggct ataagataaa ggtattttgg
     7141 aagcctgtat agtaatttca cttttgctag aaattatgga agttactata caggtttttt
     7201 gtgtaaaaag atttaatata atttagactg tattgtgtta aaataaatat ataaaaacta
     7261 tattatacaa ggagagatgt gaatttatga aacatgaatt agtacttgta atagattttg
     7321 gaggacaata taatcagttg atagctagaa gagttagaga aaacaacgta tattgtgaaa
     7381 tacttccatg tactgcaagc atagaaagaa taaaagaaaa aaatcctaaa gggataatat
     7441 ttacaggcgg tccaaatagt gcgtatttag aagattctcc aacaatatca aaagaaatat
     7501 ttgaactagg agtaccaata cttggaatat gctatggtat tcaaataatg tctcatgtat
     7561 tgggtggagt tgtaagaaaa ggtaataaac aagaaaagga atatggaaaa acatctataa
     7621 cttatggaaa atcatcatta tttgaaggaa taactacaaa tagtgtatgg atgagtcata
     7681 cagatttaat agaaaaagtt ccagaaggat ttactatagt tgcaaatact aatgattgtc
     7741 cagtagcagc tatggaaaat gtagaacgaa acctatatgg agttcaattc catccagagg
     7801 tagaacactg tttggaaggg gacaagatac ttacaaactt cttatataat atttgtaaag
     7861 taaaaggtga ctggactaca gattcattca tagaagataa aataaaagaa ttaaaagaaa
     7921 aaataggcga taaaaaagct ttatgtgctt taagtggagg agttgattct tctgttgctg
     7981 cagttcttat tcataaagca attggggaca acttaacttg tatatttgtt gaccatggtt
     8041 tacttagaaa aaatgaagga aatgatgtag aaagaatatt tagagagaaa tttgatataa
     8101 atttaataag agttaatgca gaagatagat tcttatctaa attaaaagga gtttccgaac
     8161 cagaagctaa gagaaaaata ataggtgaag aatttataag agtatttgaa gaagaatcaa
     8221 ataaattagg aaaaatggat ttcttagtac aaggaacaat ctatccagat gttatagaaa
     8281 gtggacatgg aaatgctgct actataaagt ctcatcataa tgttggtgga atcccagaag
     8341 atgtagactt ccaagaaatt gtagaaccat taagagagtt atttaaggat gaagttagaa
     8401 aaataggatt agagcttggt atagaagaag gattaatatt tagacatcct ttcccaggac
     8461 caggtctagg aataagagtt ataggtgatg taactaaaga aaaatgtgat atattaagag
     8521 aagctgatgc tgtttatatg gacgagctta gaaaagcagg tttatacaga gaaatatggc
     8581 aagcttttgc aacattacca gatgttaaga ctgttggagt catgggtgat gaaagaacat
     8641 atgcttattt agttggttta agagcagtta cttcttctga tggaatgact tctgactggt
     8701 ataagatgcc ttatgatgta ttagaaagaa tttctaatag aataataaat gaagttgatg
     8761 gagtaaatag agtagtttat gatataactt ctaaacctcc tggaactatt gaatgggaat
     8821 aaaatataga atgccagaaa tccacatgaa tacaggattt gaggtctctc ttacttctat
     8881 attatactgg tttgatacta tcaatatttg ccagtatctt atggattaat aagcaaaaaa
     8941 catcctgctg aatggtaaat tcggcggggt gtttttattt tgttttgaaa ctcctttgct
     9001 ctgaaactcg aagtatttaa cactgatgat gttgttatct tttagttcct tttgtgtcat
     9061 gctctgaaag acaaattgat acaataaaaa agtaatttct taatcattga tggagatatg
     9121 tatttatgtt actcttgatg gtaatttgag taatattgtt catagctttt tatattcact
     9181 taattagaaa accatttggt ataatcataa ataaacttga tgatataata gaaaagattt
     9241 ataattatta gaagtatata aaagtatttt atgtctatga aatgtatgtc tataaaacat
     9301 atagatgact tttgtatatt aatgtatgaa tattgatatt atagaagaat tgagttgtca
     9361 atgatactaa atcaaatatt tatagaagat ggagggatta tgagaaataa aataattata
     9421 ggaattactt tttcacttgt attactaatg tctggatgtg cttcaaaaca aacagcagaa
     9481 ataaacgtat tagcaactac tgatttacat ggagttatac cttatgagtt aacttcttat
     9541 gtaaaagaag aaagaaaaaa agataaaaat ctaactttag tagatgcagg agattttttt
     9601 gatagtggag aagtgtttgg ttctagcatg gatagttatt ttagtgctag atataaagat
     9661 ggtgataata acactgaaag ttatattgag acgcctattt caaaagatat gaaagaggta
     9721 ggttatgatg ttgcagttct tgggaatcat gaatttatat ccaacaataa attttatcta
     9781 gataatatga tatctgattt tgagaaacaa aaaataaata ttttatcggc aaatacgtat
     9841 aaaaaaaatg gagatagtta tacaaaacca tacataataa aaaatattaa tacacctgaa
     9901 ggaaacgtaa aactaggaat tttagggctt actataaaag aagttggtga gaggaaacag
     9961 tggaaagatg gaaaacttgt tgactctaag tctttagaac taaaagacca agagggctac
    10021 aatggtgaat tgtatatgaa tgatttagta gaagatgcta aaaagtgggt tggtataatg
    10081 gaaaaagaga agcctgacat tatagttgct gtggcacata caggtgagaa acctaagaaa
    10141 cctagaaacc caggaaatag aatacaagac ttagctcaac aagttgatgg aattgatgct
    10201 atagttgcag gtcataatca tgttcaaatt gagcaacatg attataaaaa taaatcagga
    10261 gaaaatgtta tagttactga gccaggaaag catggggaat atatttctaa gattaatttt
    10321 aaattagaaa aaaacaatga taaatggaat atagttgaca aatctagtaa attaattaaa
    10381 tttgaaaaaa atcaagaaag tgataaattt ggagaattta tttcttcaat taataaagta
    10441 agtaaagata agaaagaaat aagattatca gaagtaaaga ctcctgaatg ggataaaata
    10501 tatgttttta aatctggaac atctgcagat gatatatata aaacaatagg atataaatgg
    10561 agaaatatat catctccaaa gggtgatgac atggtgcaga ttgttgtaat gaataataat
    10621 aagcctgtta gctatatata tggtgaagct gaagttatgc caattaatat aaaatgtgat
    10681 gaatcagagt ataaaaataa tgttgtaact atttatccta ataaaaatga taaatttaaa
    10741 attgaaaaag gtaagcaaga atatagtatt aatttaaaac atatacaata gaaactttta
    10801 tcttatttaa gtttagattg tacttaaaat aaagtgtgta ttattttaaa aagtgatgat
    10861 aattaaaatc acaacattaa ggaatagtac acacttttta tgctaaaaaa tagaatgtta
    10921 aaaaattaaa cctttaaatt aaagtacatt gcttatatta tccaatatta tttccaaagg
    10981 ttgtgtaata tttttatcct tatgtaatat tgctaacatt ttattttcca tatctatctc
    11041 atctaattca atcaatcgca attttttatg taagagtgaa tctaataata aattttctgg
    11101 caaaatggtt atacctaacc ctgcttctgc agctttaata agtgcaaaag aattaacact
    11161 ttcccaaaca ggataagctt tttggtttga aagagataat acactgtcca aagtatcgcg
    11221 aatagcactt ccttgttcac gtaacaataa cggaaacgtg cataactgtc gtagagataa
    11281 ggtttgttca gataaaggaa aagttggagc acatgcaaca cataacttat aggaacctaa
    11341 taaaattgta cggaatgccc cctgtggttc tataccttcc caaaaacata tatcagcttc
    11401 tccacttttt aaaatatcca tagctgaatt agcactttcg acacgtacat tcactttaag
    11461 agatggaaat gaatttttta gtcgacttaa aattggtgga agtaaaaaag aggcaattgt
    11521 aatgctagaa acaatgttta taggtgtaca ttcttctaaa cgactgattt ttttgtctaa
    11581 attgcgacaa gcagttaaga tactacgtgc ttcatctaaa agagaaatgc cacagcgagt
    11641 aagacatacc cctcgtggta atctgtcaaa tagtgatgtt tcagcttgtt gttcaagttc
    11701 agctatagca tgagatacag cagattgtgt gatatgtaat ttttttgcag cacctgtaaa
    11761 agtgcctgtt tgttcaattg cttctaaaat ctctaaatat cgtatgttta tcataataaa
    11821 tatcctcact atacatgaat aaaattaatt aattccatta aattctatca ttttacagat
    11881 tgcaaagcaa caggtatact gaataatatg aagtgaaaag aaggaggaat attttgaatc
    11941 aaagcagaaa aatatatttt tggcttttaa ttattaactt gtttattagc acattcactt
    12001 ttggcggagg gtatgtagtt gtacctatga ttagacgtta ttttgtaaat caaaagcaat
    12061 tctttacaga agatgatttg atgagtatgg cagcagttgc acaatcaact cctggagcta
    12121 ttgcaattaa tttatctgct ttggcaggtt atcgagtagc tggtacttta ggggttgtca
    12181 ttagttgtac agcagctata atacctccac tcgttattct tggattagta tcagcatttt
    12241 acaccacttt tatatctaat gccattgtaa ctgcagtatt aaaaggaatg caagctggtg
    12301 ttgctgcact aattgtggat ttgattattg acatgtgctc tatgatatta aaacaacgtt
    12361 ctttgttttt atcaactatg atacctattg ctttctttgc aaactttgtg atgagtataa
    12421 atgtaatttt tatattatct gtttgctgtt ttgtatgtgt aatgcaagtt ttctataaga
    12481 agagggaaaa taaatgaata caatttggaa cttatttttt atatttttaa aaataggctt
    12541 gtttagtatt ggtggtggtt atgcaatcat acctttaatc caagagaaag tagtaaatca
    12601 aactggatgg ataagtgaga aaatgtttac agatattata actatttcac aaatgacacc
    12661 agggccactg gctgttaata cttctacttt tgttggactt caaattggag ggttttgggg
    12721 agcagttgca gccactttag gttgtgtatt atgtggtgta gtaatatctc tatttttata
    12781 taattttttt caaaagcatc agaactctac ttatatattt gaaatattaa atggtctaaa
    12841 atcagcttca ttaggtttaa ttatctcagc tgcggctaca attataatcc ttgcattatt
    12901 tgggacgaat aaagtgtact tcaattctat atttgagatg ctaaactgga ctgcactaat
    12961 aatctttttt gttatactac tgatagtacg taagtggaaa attaatccta ttgtgattat
    13021 aataataagt ggtatactag gaatattttt ttatttataa gtttttggta agtaaaggat
    13081 atctattttt ttatatctta aatccaaata gccaataaag taaggctatt tggattttta
    13141 ataaattctg aaaagataat cagaaaccca tataacattt aaattatatc actggttttc
    13201 tacaaaccaa tcataagctt cttggcaaaa tttctcacta tctatatagt aactattatg
    13261 aacattatta atttcaacaa gtttcttttt acattttagt gaattataat agctatcaaa
    13321 atattgggtt ttaaaatatc tatcatgaag tgttttaaag acaagagtag gtatgttgac
    13381 tccatctttt atcttactat cttctgcaat aaagcattct aacattgtat taattatctt
    13441 tgtactattt ttccaaatat gaggattttt cttttcaaat attttataaa attcattatc
    13501 atagacttca ttataacctt tatatttagg tggttttact gttatgtgaa aattaggaag
    13561 aaacttaaag aaaaaatata ataattttta tgttctgttt tatatattat tcatatttta
    13621 tcacagataa tatactaaag ttaatttttt tattgtacaa ctattataat aattcttcta
    13681 taatataaga cattctataa ttttatattt aagttcatgt gtatttaatt gtttttatat
    13741 aaaatgtcca gtaatactat ttggatttat ttttatttca tcaggagttc cagaagcaac
    13801 aatttcccct ccatttattc cactgtctgg acctatatcg ataatccaat cagaagcacg
    13861 tataacctct gtattgtgtt caataattaa aagtgaatgc tgttcattta caagttttct
    13921 aaaagtaatc aacaaatttt ctatatcttt aaaatgaagt ccagtagtag gctcatccaa
    13981 tatgtacagc attcgttttt tagtttttgt ccctaattct tttgctaatt ttatcctttg
    14041 agactctcct ccgcttaatc ctaatgtatt ttgtccaagc tttagatatc caagtcccac
    14101 ttcatctaat aaggagagtt ttctatatat atcagagcaa tcactaaaaa atgtttttgc
    14161 ttctgctact tccatgtcaa gtatatctgc aattgttttt cccttgtatt gtatagacag
    14221 tatttctttt tgatatctct taccttgaca ttcattacaa gttacccatt ggtctgccat
    14281 atattgtaga ataatttttg ttttacctaa tccttgacag tttgggcatt gccccttttt
    14341 gctattaaag ctaaaatagc tttcatccaa taatttttgt tttgcttgtg gagtttcagt
    14401 aaatagagaa cgaatttcat caaaaatatt gatatatgta gcaggtgtag aacggctaga
    14461 ttttccaata ggtgtttggt ccattaagat aaaatcgtca atagcatcaa atccagtaag
    14521 tgcagtataa tttttatttg ggatagattt ttgtttcaat ttttcttcta gagcaggaag
    14581 taaactgtga aatacaagag aactcttacc agaaccacta acacctgtaa tagagcacat
    14641 acagtttaag ggaatttcta catcaatatt ttttaagtta tttgcatggc atccctttaa
    14701 gcttaaccat ttacttggta taattttatt ttgtgaactt atcttattgt atgtactcaa
    14761 atatttgcct gttatagaat ttggattttt tataatttct tgggttgtac cttctgcaat
    14821 gatgaagccc ccttttgtac cagcatttgg tcctacatca ataatataat cagcagacag
    14881 tattgtatcc ttatcatgtt ccactactat aacagtatta cccttgtctc ttaattcttc
    14941 tatcattttg atgattagat tatggtctct tggatgtaaa ccaatagatg gttcgtctaa
    15001 aatataggtt aatcctacta attcacttcc tagctgactt gataatcgta ctctctgcag
    15061 ttctcctcca gataaagttg gagctgttct atctgttgat atatagctaa gacctacctt
    15121 taatagataa ctaactcgaa tttttaattc tgttaagata tcatttacca aagatagctc
    15181 atttttttga agttgatttg gtaattcaca caaccaattc cataactgtt ctatcgtcat
    15241 ttttgttagt tcagggaagc gataaccttt gattgtagtg aatctagcct caatgcacag
    15301 caattcgcca ccacatgtat tgcaaggaat tttgttcata tattgatgca aggtattatt
    15361 ttcagaacta gattctctaa aaagtctttg aatgtggttg attgctcctg aagcaggcct
    15421 tctaatcttt gattctcgtc cctttgattc aaagctaaat tcatatatct tatcgtctgt
    15481 accatataag attgcatcta taaaatctct tggcaattca ttccaaggta tatctaggtc
    15541 tatattttta tcttttgcta ttgcatataa ttcacctacc atccaattac catttggctt
    15601 tttccccctc aattttccaa aatagtaagt agctccatct aatattgaaa tagttgggtc
    15661 ttttacaatt aattgaggat taacttgata ggtaaaaccc aatccattac aatcgtgaca
    15721 agctccagta tgggagttag cattgaaggt tgaagtattt atcttagaaa aaatagtttg
    15781 gcaatgtgga caataatttt ttgtattatt ctttttttct aatggagttt tgcaatatgg
    15841 acagtaactt tttccgattg atacaaataa cattcttaag taatcccaaa tgcttgtaag
    15901 agtaccaaca gtagaacgag gattttggct tccttttttc tgttcgatag ctatagtagg
    15961 ggttaatccc tgaagtaaat caaattccgg tttttgaagc tgttctgaaa gttttgctcc
    16021 attacctaga ttttcaaaat aacgtctttt tccttcttca taaatcgtat cgaaagctaa
    16081 agaagatttt ccactaccac ttacacctgt aataacagtt atttgatttt ttggaattgt
    16141 aacctcaaca tcctttaaat tgttttgcct tcctcctttt agatagatag agcttttatt
    16201 atgacaaagt gaatttttaa ttgatttatt tattgctttt gcaaatttga atttcttttc
    16261 tagttcaaga ggattgccta attcttttat ttccgaaata cctaatattt ttaactgaga
    16321 aaagattgat ttcaaataat cttcaggcaa tttttctatt aacaaattaa ggtcttctat
    16381 ttttatattt ccgcttgata atatctctgc catttcatct aataaatttt tttctgctct
    16441 tgttaattga ttgtattttt tggaacttaa ttctgtccat aaatcccatg gaagcaattc
    16501 taaaccaatc atattattaa agtcatgtag aagattaccc tttacagaat gccaaccttt
    16561 ccaattttta ttatatccaa agtcatctgg atgagcatgt ccttgtcgac atcttatcca
    16621 ggcttctgaa ccagttaaaa aaccatctgt ttttccgaca tcatgagtgt caaaattaat
    16681 ttgaaaataa tttttttgta tgtcatctat ttgtgcatct attcttatcc atctctttgt
    16741 tagagtatca tagtattcta caagccagtg gtcttcataa gtaagctcat tttcaaaata
    16801 attagcaaaa cctgctctca ttctagcctc aaaacctcga tatcttagga gcgatacaag
    16861 aagtaaagaa taatctctgc acattccaac aagcctttgt tttgggtttc ttggaatagt
    16921 tagtgggtta gaatttattt caaatattct ttttaacatt tgaggaattg tacgaagcaa
    16981 ttcctcatca gattgttgct tattaaagga tataccatat aattttcctt ggtctccatg
    17041 aattactagg ctttgaacca cttttaataa ctcaattgga gtctttggta aatcttgata
    17101 tttattttca aattttttag gatttgtgat gatgctttga tttttatagt tatttatttt
    17161 catttatatt tcacctctct aaatggatga tataccctga catggtgtca tatgcaagga
    17221 caatttagat tttttggcca aaatttaaaa ataaataaaa aatgatacag tgataaattg
    17281 taatacaaat atagtttcaa attgcatata atataattat ttaataaaac cattatctat
    17341 ggaatgttaa tgaaatgtta agatggcatt cttgaatatt aagaaataga atactataat
    17401 atagtgtgaa ggattcttag taaaaaaatt tgtaagtgtt aatatacatt aaaaaatagg
    17461 tttataattt ggaggaaaaa aatgaaatta tcaaggtata agaaaaatag tttagttctg
    17521 tctataattt tatgttcact tattatttca atccttgtaa ttactgtttt taataccgta
    17581 aaactacaca atatatctaa taaacaatct aaaaactatt taaacgatgt atctacacag
    17641 attgtaatga atgttgattc taaaatcaaa tttattttat ccgacttaag aataatggca
    17701 gattttatta agcaatatga aggtgatagt cgatatgagt atttatctaa aagaaagact
    17761 agttataagt attatgatgt tggaataata gatttagaag gtaaagctga gtttttaagt
    17821 ggaaaaaagt ttgacttaaa gaatactgct tcctataaaa aaggaattga aggtaaagaa
    17881 tactcagaag taatagagtc aatgggtttt gttctttata gtgtacctat atttaacgat
    17941 aatcatgaag tgtctagtat tttaataggt gtatccaata aagaaagcat ggatgagatt
    18001 cttaatatag atagctttaa tggagaagga actattgaaa ttattaatag tcagggaaaa
    18061 actcttttca taggaaaaaa taatgagtta ataaaagacc ttagctataa gtataaggat
    18121 tctgcagatg aaccttgggc acaaaagatg cttaatgatt ttaaaaatga taaatcagga
    18181 aatattacaa taacctcatc aaaaggtgta caatgcttat taacttatca tccaattaca
    18241 agaggtttaa atgattggca ttttcttctc atagtacctg aagatgttgt acttggtgaa
    18301 cttaataagt taaattcttt tacaataata atgacattta taactacatg tattattgga
    18361 attataacat taattttata tattataaga agaaaatatg taaatcaaat agaaaatatt
    18421 gcatatttag atagtataac aaatggtata aattcaacta agtttagtat gctagtcaaa
    18481 cctttaattt cagaatcacc tgatagtact tatatgatga tagcaatgaa tataaaagat
    18541 tttaagctaa taaatgattg tttcggtagt gaaaaaggaa atatgacact aaaacatctt
    18601 tacaatattt tgtataaaaa cattgatgaa aatgaagaat ttgtgtgccg tcatgatgca
    18661 gaccttttct atttgcttat taagaataga ccagtgttag aagccctgga gttattatat
    18721 aaaatagaag ctgacacaaa ttattttaat aaaaataggg aaaatccata ctttttaaga
    18781 ctatcagtag gtatttacac aattgaaaat cataatgaag atttaattac aatacaagac
    18841 catgcaaata cagcaagaaa aagctttaat aaaactcatc aaagtgattt ttctttttat
    18901 agtgatatag aaagaaaacg attaatatct gaaaaagaaa tttcaaattt aatggaaaaa
    18961 gcccttgaaa ataaagaatt ttttatgtgc ttacaaccta aaattgatat aaaaagtgga
    19021 aaaattagtg gagcagagtc tcttgtgcgt tggaaaaatt cagaaaaggg cataatttat
    19081 ccaagtaatt ttattcctct tttcgaaaaa agtggattta tatgtaagtt agatttatat
    19141 atactagaag aaacatgcaa attgatttct aagtggataa aagaaggtaa agaagtgttg
    19201 agaatttctg ttaatgtatc acgtcaacat ttgaatgata aattgttttt agaaaaatat
    19261 aagcatatat gcaataaata caatgtacca acatgtctga tagatttaga acttaccgaa
    19321 tctattttct tagaaaatcc agaagctata gatataatag aggatattca ttctaatgga
    19381 tttaaatgtt ctatagacga ttttggattt gggtactcat cattagggat attaaaggat
    19441 tttaaagttg atataattaa gttagaccgt tcattctttg ttagcaaaaa taatatagac
    19501 cgtggtaagg tagttattaa gtctattatt gaacttagca aaaggctagg tatgaaagta
    19561 actgctgaag gaattgaaga attagaacaa gttgagttcc taaaaaatgt aggatgtgat
    19621 tatattcaag gttatgtttt ttctaaacca ttattaattt ctgattttga aaattttgca
    19681 tatgaaaata ataaaattaa aaatatccat tattaaatgt acaagataaa aaatttaggc
    19741 aagttatata attaaaaata taacttgtct tttttattta tatatttaat aaaaatatat
    19801 ttgcagattt tgtaaaaata attttcaaat gttgtaagtt gtggctaaag ctttattgta
    19861 ttattctgaa tatagaaaga tagttactgt cttagataaa aactgaaaca tacataataa
    19921 tataaaattt gctatattaa taactctaat tattttggaa ggagggaaaa tatgataagt
    19981 gggagaatgt tactcatcat gaattttcta aaaggaaaaa ataagacaag ttacaaggat
    20041 attagcaaac aacttagtat agaagaaaga aaagttagat atgatataaa taatttaaat
    20101 attgttttgc gttcattaaa caagtcagta atacaaaaga tgaacaaagg tgtgttaatt
    20161 attcctgatg acttccaagt agtatgtata aataataacg aatatgtatt ttccttaact
    20221 gagcgagtat ctattatgaa aattatatca atgtttaaaa tagataatct aaacttagaa
    20281 aaattgagta aaggatttca ggtttctaga agttcaatta aaaatgacct taatgttttg
    20341 tcaagtgaat tagaaagaaa ccaaataact attacttatg ataaatcttt taaaataagt
    20401 ggagaagaag accttatata taagcaaaga ttgaacatat taaaatcgta ttcttatctt
    20461 tttggtaagg aaaaatctga tttaagtgta tttgagaagt atttagtgaa tgtaattgaa
    20521 aaaatattca aagggattta tttgattgat atatataatt ggtctatgaa tttattattg
    20581 caaatgggat ggactttaaa tgatgaatct tttacatggt atgtgtcaaa cattttcctt
    20641 tttacatggt atatacattc agatgaaaaa catcctttag aatctgcttc attaagtgag
    20701 ccatgttttg aaaataagtt tgaccaagaa tttgaaacaa ttattcaaaa aaagttgact
    20761 aaggaacaaa tttttctgtt aaagagtttt gtatttttta caaataaata tgcaagttta
    20821 aacgaggata tggatttgat tagtacagaa attatagttc aagacctaat ttacaatatg
    20881 tcagaaatat tagagattag ctttaaagaa gatatgattc tttataaagg attactcaat
    20941 catattgccc cattgataga gagaatcaag cagaatatac agatttatga ggaattactt
    21001 catgtaatac cagataaata tcaatatgta cttgaagcta ctaaggtttc tattcaaaaa
    21061 aacagagttt taagtcaaat aaagaatgaa aatgaaataa tacttttggc aatacatttc
    21121 ttaggaagta tacatagaaa tgagaggaat aactacaaaa atatattgtt agtttgtgga
    21181 tttggatatg gtgtaattgc tatgttaaag gatagtttag taaatgatta ccagataaat
    21241 atagtagaga gtattccatc atataagtta caagattata aagaatggga aagtattgac
    21301 ttcattataa caacatcaaa gatagtaata gacttgccta aaacaattat tgagataaat
    21361 ccatttttac aagaagagga ttatttgaag tttgaagaaa aagggataaa aagaaagaac
    21421 gtcttaacta attatatgtc aataaaaaaa aggttggact ttttagaaga agatgctcaa
    21481 aaaagagtat taagtattat atgtcaagaa cttggataca gtgatgagag aatattattt
    21541 agacaactta atttaagtga tttaataggg attgatacca taaggataat tgatgaagat
    21601 atgaaatggg aagatgctgt aaaaattagt tcaaacatac ttgctaattg tgattttgta
    21661 tcagcacaat atgctgagaa tattatagat attttaaaaa atgtgggttt ttatgctgta
    21721 aaagataacg aatttgcttt actacatggc aatgatagtt cattagtcaa agtaagttcg
    21781 ataagtttat taatatgtaa agaagcagtt agttttggag ataaaaaggt taaaatcata
    21841 ttttgtctag caagcagaga taaaaaagag caaattcctg caataattaa tctaactaaa
    21901 atggtttata agactaattt tattgctatg ttagaaagtg caaagacaaa agaagaagca
    21961 gaagttttaa ttcataaata tgagaaggag gtcactgtat gaataaatta gttaatgtaa
    22021 aatgttcatt taaaatacat gctaattcaa aagaagaagc tattgtatcg ttagttgatg
    22081 tagtatcaaa agaaggatat ctaatagata aaaatcaatt tttggaagat gttttaaaga
    22141 gagaagaaac attgtcaact tatattggac atggtatagg tcttcctcat agtcaaagtg
    22201 taggtgttaa aaactcttgt attacaatag gaaagcttga tattcctatt gaatggacag
    22261 aagaaggaga aaaagtggat ttaatatttt taatatcagt aacaaaagac aatgaaaata
    22321 atcttcattt aaagatttta tcaaaattag cacgtttatt gatgcatgaa agttttagaa
    22381 atcaaattag atgttcagat gaacaaactg tttataatct tataaaagaa aaaatagagg
    22441 aggaagatta aaatgagaat tttaaagttt ttgcaaaaac atcttatgac agcaacatct
    22501 tatatgattc catttgtggt tgcaggagga atattatttg ctttatcagt aactctaagt
    22561 ggacaagctg ctgttcctga aacaggctgg ttggctaaac tgaatcaaat tggagctgct
    22621 ggcttggctt tattcattcc tatattaggt ggatatatag catttagtat ggcagataaa
    22681 ccaggattag ctccaggtat gataggtgca tatttagcaa aagaagtaaa tgcaggtttt
    22741 ataggtggta tcatagcagg atttatagca ggatttgttg ttttacaatt aaagaaaatt
    22801 aaattagctc ctacaatgag aactttaggt tctatattta tttatccttt gattggtaca
    22861 ttgattacag gtggtataat tgtattttta attggagaac ctatagcttc ttttatgaca
    22921 tggatgacaa actggttaaa tggaatgtct ggtgtttcta aaattccttt aggtggaatc
    22981 ttaggaggaa tgatagcctc agatatgggt ggtcctataa ataaggttgc tgctactttt
    23041 gcccaaactc aagttgacac acttccatat ttaatgggtg gagttggcgt tgcaatttgt
    23101 atacctccaa ttggtttagg attagctaca ttattgtttc caaaaaaatt tagcaaggaa
    23161 gaaagagatt caggtaaagc ttcgctatta atgggatgtg ttggaattac agaaggagct
    23221 attccatttg caacatcaga tccagttaga gtaatacctt gtattatggc tgggtcgatt
    23281 ataggaaata ttatggcttt tttattagga tgtttaaatc atgcaccctg gggaggtctg
    23341 atagtattac ctgtagttga caataggttg gggtatattg catcagtatt aactggagca
    23401 gtagttgttg cagtattaat gaaattagta aaaaaagacg tgaaagaaga tgaagaaatt
    23461 gaagaggatt tagatgaatc aattgaatta atatttgaag aattataaat ataaatggaa
    23521 ggaatttgta taaaaaagta aatataaaac tataaactta aaggagataa gaatatgaaa
    23581 attgtagcag taacatcttg tcctagtgga gtagcacata cttacatgtc ggctgaatca
    23641 cttgaattat cggccaaaaa atttggtata gaaataaagg ttgaaactca aggaagctct
    23701 ggaatagata atgaattgtc acttaaagat attgatgaag caacttgtgt aatattgact
    23761 aatgatgttg agattagaaa tatggaacgt tttaagggaa agaaggtagt aagaatgagt
    23821 gtatctgaga taattaagaa atctgatgca ttaattaaga aaataaaaga tacttttcaa
    23881 taaaaattga tttattttac atataactca aagatgtttt taaagaaatt tttattaaaa
    23941 gcatctttga aaatatctta aaaatagaat aaaaggagat atagtcatga aaaaattacc
    24001 aataaagact gtcgtacaaa gattactaga cttacaagaa gaggggaaaa gtgcaactct
    24061 tttaggaatt ggtcctatgt caccaaactt gttacaagca agttttgaac tagcaaaaga
    24121 tgatgatttt cctttaatgt ttatcgcaag tagaaatcaa gtagatgcag atgaactagg
    24181 tggcggttat gtaaatggtt ggaatcagga aacttttaca aaagatatta agaagattgc
    24241 cgacaaagtt ggatttgatg gtttatatta tttatgtaga gaccatggtg ggccatggca
    24301 aagagataaa gagagaaatg accatttgtc tgttgatgaa gcaatggagt taggaaaaaa
    24361 atcttattta gcggacattg aagcaggttt tgacctattg atgattgacc ctacaaaaga
    24421 cccatttgaa attggaaaag taattccact agatgtagta ctagaaagaa ctgtagaatt
    24481 aattgagtat tgtgaaaacg agagaaaaag acttaatcta ccagacatag gttatgaggt
    24541 tggtacagag gaaaccaatg gaggtcttac ttcaactgag acgtatgaaa catttataac
    24601 tagactaaag gttgaacttg ataatagggg tttgccaatg ccaacattta tagttggtca
    24661 aactggtact ttaactagaa agacagaaca agtaggaact tttaatttta gaaatgctta
    24721 tgatttagct caaatggcta agaaatatgg agttggttta aaagagcata atggcgatta
    24781 cttagatgat gtaactttat tagaacatat accatctcaa attattgcta caaatgtagc
    24841 tcctcaatat ggtactgaag aaactagggc atatttaaaa ttagcagaag ttgagtgtaa
    24901 attagaaaaa gagggcctag ttgataaaac ttctaatatc agacatgttc ttcttgttaa
    24961 tgcaattgaa tgtggacgtt ggagaaaatg ggtagtagga gaacaaaaaa atctaactac
    25021 tgaagagata tttaagaatg aagttttatc taatgaaata ctagatattg caggacatta
    25081 tacatttaat aatgatgatg ttaaaaaaga aatagaagtg ctttatgata atttaagtaa
    25141 gaataatatt gatggtcaaa gatttgttgt agaccatatt aaacgtcctt tgcgtgatta
    25201 tgcagaatgc tataacttaa aaggtgttac tacgaggatt ttaaataaat aataggactt
    25261 atagtggtga gtattgttat actaggtatt tatagttata attatactta ccactattaa
    25321 atttaggagg actaatattt tgattaattt aatttttgat gtagatgata ctttatataa
    25381 ccaattaacg cctttttaca ctgcatataa taaagtgttt tcttcaataa aggatatttc
    25441 tattgaagat ttatatatga gtagtagaaa gtatagtgat gaggtatttc atatgactga
    25501 aaatggagaa atgccaatca aagaaatgca tatttatagg ataatgaaag cttttgagga
    25561 gttgggaaat tctattacag aaaaagatgc acaaagtttt caagatgagt atatatatca
    25621 gcaatcacaa attacactaa taccagaagt tgagaggatt ttaaattttt ctaaagaaag
    25681 aaatattaat ctaggaatta taactaatgg cccatcaaac catcaaagaa tgaagttaaa
    25741 gcagttaaat attgaaaatt gggttgataa aagtaatatt tttatttcta gtgaggttgg
    25801 attttctaag ccagatacta atatttttag agtagctgag aatgttatga atttagatag
    25861 agaaaataca tattatgtgg gtgattctta tagaaatgat gtattaggtg ctaaaaaagc
    25921 tggttggaag agtatatggt tgaaccatag aggacatgag gtagaagagt tgttttataa
    25981 acctgatttt gtaattttgg aacataagga tttgatatct ttatttataa aaatatgtag
    26041 ttataaaaat atgtagtaaa aaaatgttaa taatagaatt gtataaaata attaataata
    26101 actatataaa attatatgtt tagattgtga gcaatctatt ttatgttaaa aatagattat
    26161 ttagaagaag gggaaatctt aaaaaaattt gaatatcccc tttttattat ttaattaaaa
    26221 tgaaatagtt agaaatatat tttatttagt ttagtcaata acattttggg attctttata
    26281 actacatgtt aactgttgaa taggcttatc atatggttta acccatcctt tattaatact
    26341 taattctgta agtgctgtgt gaccttctac catttgagtt agtgaagctg aatatatagc
    26401 tcttaattca ggtgtactac ttgttaatgc agaattaaga tacatattgg cagatgcttt
    26461 agcagatgaa agcatactta acattattac tttatcgtct atataagtat tgttcttaat
    26521 tagattttct atcatatttc tcatgttatc tatacctcct cagaaattag tatttcattt
    26581 tcaactataa attgttgtat agctttaatt cttccttctg ttgctaatat actagattct
    26641 gattgtcttt ttaggtcttc atctgatatt aacatattta ttgctctttg cataagtaat
    26701 ccgtcagact ccatttttat aactgctgct aaagatattt tttcagtttc tgaaagtgtt
    26761 ctcatttaat catctcctat attgtaattt atgctttaat agtatgtctt tcttttcaag
    26821 gaaataacca ttacactttt ttacagtatt ttatattctc tttatatcaa agtttagtca
    26881 acttaatata ttaatataat tttgtaggat atttatcatt aaatatctat aaaataaatt
    26941 atttgaatat tcataaatca aaatttattt gtatgtactt cttcaagagt caatatgaat
    27001 tttttcaaaa aattaatttt gatgataatc attaagggct atatttttcc taaagttgtg
    27061 attatcatta ttaatttact aaaatacttt aatgaaaaat agctaacttt aattgtagga
    27121 tattcttaat atatttgaaa taaataaaaa tattgaagga tataatattc aattggtata
    27181 taaataataa atgatagtta agatttacct tagaggctaa ttaggtgttc agtcaaaatt
    27241 aatgccttta gaatacaaat gtattctaaa ggcattttta ctatgtattt tcatttatat
    27301 tactaaacat tatctatttt tatatacatt tctatgtatt ttattttcat caaatcggtc
    27361 tataaattca tttttttctt cactatatcc aatagatata agtgcataag gaactaattt
    27421 tactggtaag tctaaaattt gtttaacttt atttactact ttatttttag gaaatactcc
    27481 tgcccaacaa gcagctagac cttgattagt aacttctaat aataaatttt gtgtgcaagc
    27541 acctaaatct tgtatccatt taccaggttt taagaattta tctctatttc caagcactac
    27601 tatacataat ggtgcaaatt ctatatgctt tgctctatgc tgtgactttg agagctcttt
    27661 cagcagttcc ttatcatcta ctataataaa ttcccaaggt tgagcatttt ttgatgaagg
    27721 tgcttgcata gctgatttta gtatttcagt tattttatct tcttctatat ttttatctat
    27781 atatgttctt attgaacgtc tattttttat aacttctaac atacagattc tcctttaaat
    27841 aaaattattt ctatttatat aatatcatta ctttaagttt tagctattac ttatataaag
    27901 aataaacttt gtgttggttt tatctaagta aaagtaacat tcagaactaa aaaacaagac
    27961 tataaatcca agatattaat taaaaaagaa gtaataaaaa ataatataca agatttaaaa
    28021 aagagtattg agcttaaaat ttaattagaa aaccaatttt gagataatct gttttttatt
    28081 ttaacaaaaa aacataaata aagttcgata cgtagcgaat gaaaaaaata caagaaatat
    28141 tttttttaaa aaatttcaaa aaagagttga taatatatgt atattaatat aaaatttaag
    28201 gtgtataatt cgtataataa tacgaatgat ttgtagtgta aaataactta taaacggaga
    28261 ggggaagtct aatgaaagta gcagtagtta tgggttcaaa atcagattat ccaaaattag
    28321 aagaagggat aaaactgctt gaaaaatatg gaatagaggt tgtagcaaga gcattatcag
    28381 ctcatagaac accagaacaa ctttcaatat tcctaaaaga aatagaagat gatactgatg
    28441 taataatagc agcagcagga aaagcagcac acttaccagg tgtaatagct tcacaaacat
    28501 taattcctgt aataggatta cctattaaat catcaactat ggatggacta gactcacttt
    28561 tatcaatagt tcaaatgcca aaaggaatcc cagttgcaac agttacaata gacttaggtt
    28621 taaacgcagc tttattggca ttacaaataa tgactttaaa atacccaaaa ttaaaagagg
    28681 atttgaaatc atatagagaa gagatggcac aaaaggtgtt agaagacgac aagaatttaa
    28741 gggggtagca gaaatgttat tatatgaagg aaaagcaaaa caagtatatt ctacagataa
    28801 tgaaaatgaa tatgttgttt attataaaga tgatgcaaca gcatttaatg gagaaaaaaa
    28861 agcagaaata tcttcaaaag gtatactaaa taataagata tcaacaataa tctttgaaat
    28921 gttaaaagaa aataatataa atactcactt tataaaaagt ttatcagata gagaaatgtt
    28981 agttaaaaaa gtagagattt taccattaga agtaatagta agaaatattg ctgctggttc
    29041 tatctgtaaa agagttggtc ttgaagaagg tgtagttttc gatgagccaa tttttgaaat
    29101 aagttacaaa aatgatgctt atggtgaccc aatgctaaat gatgattatg cagtggcaat
    29161 gaagttagca actagagaag agttaaaatt cttaagagaa gaaactttaa aaataaacga
    29221 gttactaaaa gcatttttct taaaattaaa cctaaagtta gttgacttta aaatagaatt
    29281 tggtaaggat tctgaaggaa atataatatt ggcagatgaa gtatcaccag acacttgtag
    29341 actttgggat gtaaatacaa atgaaaagtt agataaggac agatttagaa aagaccttgg
    29401 agaccttgta gaaggttata cggaagtact ttcaagaatg aataataaat aggaggcaaa
    29461 tgtatgtgtg gagttttagg gatttactct aataaagatg taactaaaga actatattat
    29521 tctttatatt ctatgcaaca cagggggcaa gaaagctgtg gtttagctct tttagatgat
    29581 ggagaaatta agtacaaaaa agatatggga ttagttggag atgtttttaa ggaaaatgaa
    29641 ttatctaagt taaaaggaaa tatagggatt ggacatgtta gatattcaac agctggagga
    29701 agtcatgttt ctaactgtca acctttagtt ggaagttgta gaaaaagaca attagccata
    29761 gctcataatg gaaatctagt taatgcaaat tatttaaaag atatgctaga agaagatgga
    29821 tatatgttcc aaactaattc agatacagaa gtgattttat atatacttgc tagatattac
    29881 aaaggggata tagtagaaag tttaaaagtg acaatggatt atataaaagg tgcatatgct
    29941 cttgtaataa tgagtcaaga agaattggtt gcagttagag acccacatgg attcagacct
    30001 cttgtacttg gtaaaaaggg tgatgaatat atatttgcat cagaaaattg tgcaatagat
    30061 atacttggcg gagaagttat aagagatgta gagccaggag agataatagt agtcaaagat
    30121 ggagaactaa aatcatattt ttattcagaa aattataaac cagttaagaa aagttgtata
    30181 tttgaacaca tatactttgc aagaaatgat gcaactatag acaatgtaaa tgcatatgaa
    30241 tttagaatta aatgtggtga gagattagct cagaatgaaa ctgtaaaggc agatatggta
    30301 gttccagttc cagattcagg ttggccagga gctattggtt atgcaaatgc tagtggatta
    30361 aaaataagtg aaggcttagt taaaaataga tatgtaggaa gaacatttat aaaacctaca
    30421 caagaagaaa gagaaatagc agtaaaaata aaattaaatc ctctttcaac aataataaaa
    30481 ggaaaatcta taattttagt agacgactca attgtaagag gaactacatc taaacaatta
    30541 gtaaaatctt taagagaagc aggagcaaaa gaaattcatc ttagaataac ttctcctcca
    30601 gtagcttact cttgttacta tggaatagat actccaaatc gctctaaatt aattgcatca
    30661 agtaacaacg tagaagaaat gagagaatat attggatgtg atagtttaaa attcctagat
    30721 atagaaggaa tgttagatgc aacagaacat aagtctactt tctgtaaagc ttgttttgat
    30781 ggagaatacc cagttaagaa aattgataag gaggaactac tttcatgtta acatataaag
    30841 aatcaggtgt agatatagat gaaggaaata gagcagtaga ccttataaaa ggtaaaataa
    30901 aaggaactta tgatggaaat gtagttggag atttaggtaa cttcagtgga ttatacagtt
    30961 taaaagattt tgtaggtatg aaagaaccag ttttgcttgc atctacagat ggtgttggga
    31021 ctaagttaaa aatagctcag atgatggaca agcatgatac tgttggaata gatttagttg
    31081 caatgtgcgt gaatgattta atttgtcaag gagcaaaacc attatttttc cttgactata
    31141 tagctcttgg gaaattagtg cctgagcata tcgaaaaaat agttggtgga attgcagatg
    31201 gttgtaaaat gtctggttgt gcactaattg gtggagaaac tgctgaaatg cctggaatgt
    31261 atggagaaga tgactatgat ttagcaggat tttctgtagg aatagcagac aaagaaaaga
    31321 ttgtttctgg aaacaatgtt aaatcaggag atgtattggt tggtatatct tcaagtggag
    31381 tacacagcaa tggattttct tttataagaa agatattttt agaaacttac aattataaaa
    31441 tggaacaata tgttgaagaa ctaggaatga cagttggaga agcactttta acaccaacta
    31501 aaatatatgt taaattagct ttagatgtat tagctaaaca tgacataaaa gctatagctc
    31561 acataactgg tggagggctt atagaaaata taacaagagt tatacctaaa gggttaggac
    31621 tagatatcaa taaaaaatct tgggagaagc ctcctatatt taaaatgata gaaggtttta
    31681 atgctgttga tgagagagaa cttcataaga gttttaacat gggtattggg ctagttttaa
    31741 tagtagataa agaaaatgct gatgatgttg taaactttat aaataataga gaaaatgaca
    31801 atgctgctta tgtagataaa aaatacagtg aattattaga agataaagcg tatataattg
    31861 gtgaagtagt agatagtcat gaaggtgtag aattatgcta aatataggag ttttaatatc
    31921 tggtggagga acaaatttac aagcagttat agatggcact gagtctggag aaataaaagg
    31981 tcaggttaaa gtagttatct caagtaagca aggtgcttat gggctagaga gagctaaaaa
    32041 tcataacata aaagcaattt gtgaaactga tgaagacaag ataatagaga ttcttaagga
    32101 aaataaaata gatttagtag ttttggctgg ttatttaaaa ataatcagcc caaagctggt
    32161 taatgagttt agaaacaaaa tgataaacat acatccatca ttaattcctt cattttgtgg
    32221 agctgggttc tatggagaaa aagttcatca aggtgttata gattacggag ctaaagtaac
    32281 tggtgctaca gttcattttg ttgatgaagg tgctgatact ggaccaatca taatgcaaga
    32341 tgtagttaag gtaaatcaag atgatgatgc aaagacactt gcaaaaagag tgttagaagt
    32401 tgaacatagg attttaaaag aaagtataag tcttttttgt gaaaataaat taaaacttca
    32461 aggtaggaga gtgtttataa atgagtaaga gagctttaat aagtgtaact gacaagactg
    32521 gtgtagtgga atttgctaag gagttaaata agttagatta tgaaattata tctacaggaa
    32581 atacatttaa aacattaaaa gaaaatggtg ttaatgtaat gcaagttgaa gatgttacaa
    32641 attttccaga aatattagat gggagagtaa aaacattaaa tccatatata catggtggta
    32701 tactttataa aagagataaa gaatctcatg tagaaactgt gaatgagcat aaaatacatt
    32761 caatagattt agtagctgta aatctatatg attttgaagg aactttaaaa gctggaaaaa
    32821 gtcatgatga aataattgaa aatatagata ttggtggacc atctatgata cgttctgctg
    32881 ccaaaaacta taaagatgta atagtagtag tagatataaa agattatgat tctataatag
    32941 aaaaattaaa aacagataca atgacattag aagacagaaa aaaattatca tataaagctt
    33001 tctcaactac aggaagatat gatgcgttaa tatctagtta ttttgctgga gaagttggag
    33061 atacatatcc tgacatatta aatttaactt tccaaaaaga acaaacttta agatatggag
    33121 aaaatccaca tcaaaatggt ttcttatatt cacaatcaaa tgcaaaaaat ccaatcttaa
    33181 attatgagca attaggcggt aaagaacttt cttttaacaa tttaaatgat ttacatggat
    33241 gtttagaagt aatgagagaa tttaaagata gtgaagaagt agtttcagta gctataaaac
    33301 atgctaattc atgtggggta ggtctaggaa aagatgcttt tgaagcatat acaaaatgtt
    33361 atgaagcgga taaggtttca atatttggag gaatagtagg aataacttct actatagata
    33421 aagctacagc agagaaatta aatgaaatat tcttagagat agtagtagca tatgattttg
    33481 agccagaagc tttagaggta cttaaacaaa agaaaaattt aagaatatta aaattagcta
    33541 agatagaaga tagtttacaa ccatatgaaa tgaaatattt agatggaaaa ttattaatac
    33601 aagatagaaa taatatatta gcagaaaaaa gtgaaaatgt aacaaaagca aaaccaacag
    33661 atgcacaact taaggacatg gaatttggta tgagagtagt aaaaaatatg aaatcaaatg
    33721 caattgcgat agtaaaaaat ggtcaaactt tagctttagg ttgtggacaa acatcaagaa
    33781 tttgggcatt aaaaaatgct cttgaaaata acaaagataa agattttaca ggagctgttt
    33841 tagcgtcaga tgcatttttc ccatttgatg attgtgtaac attagctcat gaatatggaa
    33901 tatcagcagt agttcaacca ggtggctcta taaaggataa agattcaata gaagcttgtg
    33961 ataagtatga tatggttatg gtatttacag gaataagaca cttcaaacac taaggaggat
    34021 attatgaaga tattggttgt aggcggtggc ggaagagagc atgctatatg ctggaaacta
    34081 agcaaagaaa aaaatgtaga aaaagtttat tgcgcgccag gtaatgcagg aattacaaat
    34141 gtagcagaat gtgtaaatat aggtgatact aatatagaag aacttttaaa atttgccaaa
    34201 gaaaatgaaa taggtctgac aatagtagga ccagaagtac cactagttat gggaatagta
    34261 gatgagtttg aaaaagaagg attaagagta tttggaccta ataaaaaatg tgctcaactt
    34321 gaaggaagta aagctttttc aaaagaattt atgataaaac ataacatacc tactgcaaaa
    34381 tataaagaat atacaaactt agaagaagct atatcagaaa tagatagttt tggatatcca
    34441 gtagtaataa aagcggacgg attagctgct ggaaaaggtg ttgtaatacc tgaaaataga
    34501 gaagatgcta tagcaacttt aaaagaaatg atgtcagata agaagtttgg agctgctgga
    34561 gataaaatag taattgaaga gtttttaaaa ggaatagaaa cttcaattct agcatttgta
    34621 gacaatgata caatagttcc tatggcaagt gcaaaagacc ataaaaaagt aaacaactat
    34681 gagcaaggtc caaacactgg gggaatggga actttttcac ctagtgaaat atatacagaa
    34741 gaattagcaa ataaagtaaa agagacagta ttagaaaaaa ctttagaagg cttcaaaaaa
    34801 gatggactta attttaaagg tattttattt gttggtctta tgataactga agatggagaa
    34861 aaagtacttg aatataatgt aagatttgga gaccctgaga cacaatctgt tttatttaga
    34921 ttagaaacag atttacatga gataatggaa actatactag ataataagtt aaaagatata
    34981 gaaataaact atagtgatga tgaagctgta tgtgttatgc ttacatcagg agggtatcca
    35041 gacagttatg aaaaagggaa aattataact ggtcttgaaa acttggatga tgatatagta
    35101 gtattccata gtggcactaa gatgcttgat ggtaatttag ttaccaatgg aggaagagta
    35161 attggtataa ctgcaaagtc aactacagtt aaggatgcag cagaaaaagt ttatgaaaac
    35221 ataaagaaga taaactttga aggaatgcac tatagaacag acataggaag ataatttttc
    35281 taaaatggag gataaggtat gttgaatact gaaaataaag actcaatggt tagaagagta
    35341 ttagttgaaa aaagagaagg ttttgacctt gaggctaaag ctttaaaaaa agacttagta
    35401 gaaagtttac acatagataa tatagaaaat ttaagaatat taaatagata tgatgtagaa
    35461 ggaatatcag aagaagttta tgaaaatgca gcaaagacaa tattctctga accaaattta
    35521 gatgtagtat attatgaaga aataccaaaa ttaaatgatg aaagagtatt tgcaatagaa
    35581 tttcttcctg gtcaatacga ccaaagaggt gattgggcag ctcaatgtgt acaaattgta
    35641 aatcaaggga taagacctgc tattaataca gcaaaagttt atatattatc aggcaaaata
    35701 actgatgaag agttttctaa aataaaagat tactgtataa atccagtaga tagtagagaa
    35761 gcatctttag aaaaaccaga aacattaaaa atggaaactg aaataccaac aacagttgaa
    35821 gtattagatg gatttataga tttagatgaa aatggattaa gaacatttgt aagtgaaaaa
    35881 ggattagcaa tgactttagg agacttacaa cacgttcaaa aatactttaa agatacagaa
    35941 aagagaaatc caactataac agaaataaaa gtattagata cttattggtc tgatcattgt
    36001 agacatacta cttttatgac tgaaatagaa aatgtaaaga tagaagatgg taagtttaat
    36061 gatatagtaa aagaagcgta tcaaatgtat cttaactcaa gagacaatgt atatgtgaac
    36121 agacataaag atatatgttt aatggatata gccactgttg cagtaaaaga attaaagaag
    36181 aatgggaaat taaatgacct agatgaaagt gaagaaataa atgcatgtag tataaacgta
    36241 gatgttgaag ttgatggtaa gatggaaaaa tatctagtaa tgtttaaaaa tgaaactcac
    36301 aaccatccaa ctgaaataga accatttggt ggagcagcta cttgtttagg tggagctata
    36361 agagacccat tatcaggaag aagttatgtt tatcaagcaa tgcgtgttac aggaagtgct
    36421 gacccaagaa cgactttaga agatacttta cctggaaaac ttatgcaaag aaaaataact
    36481 acagaagctg ctcatggata tagttcatat ggtaaccaaa taggtcttac aactggacaa
    36541 gttgcagaag tatatgatga aaactttgtt gcaaagagaa tggaaatagg tgcagttata
    36601 gcagcagtac caaaagagaa tgtagtaaga gaaagacctg aagcaggaga tgtaatagtt
    36661 cttcttggag gaaaaacagg aagagatggt tgtggagggg caactggttc atctaaagaa
    36721 catagtgaag agtcaatctt aacttgtagt gcagaagttc aaaaaggaga tgctcctaac
    36781 gaaagaaaaa tacaaagatt ctttagaaat aaagaagttg ctcaaatgat aaaaagatgt
    36841 aatgattttg gagcaggtgg agtttgtgtt gctataggag agattgctga tagtttagat
    36901 ataaacttag acttagtacc taaaaaatac gatggacttg atggaactga gcttgcaatt
    36961 tcagagtcac aagagcgtat ggcagtagct ataaaaaaag aaaataaaga taaatttata
    37021 caattagcag tagaagaaaa cttagaagct acacatgtag ctacagtaac agatacagga
    37081 tatttaagaa tgttctggaa tggaaaagca attgtagata taaatagaga gttcttagat
    37141 acaaacggag taaaacaaac tactgatgtc catgttacta aagtagatga agaaaataca
    37201 ttctttagct ctaatgaaat agtaaaagat gttaagtgtg caagtatgaa agacaagttt
    37261 acaaaagtat tatctgacct taatgtttgt tctcaaaaag gattagtaga gatgtttgat
    37321 aatacaattg gtggtaatac agtacttatg ccatttggag gaaagtatca agcgactcca
    37381 acacaaggta tggttgctaa gataccagta cttggaggag aaactaatac ttctacaata
    37441 atgacttatg gatataatcc aaaggtagga aaatggagtc cattccatgg tgccttgtac
    37501 gcagtagtag aatctgtgtg taaattggtt gctattggag gaaattatag tacaacaaga
    37561 cttacattcc aagagtattt tgaaaaactt ggtaacaatc cagaaaaatg gggtaaacca
    37621 ttctcagcac ttttaggagc attttatgca caaagtaaat ttgagatacc tgctataggt
    37681 ggaaaagaca gtatgtctgg tacatttaaa gatatcgaag ttccaccaac attagtttca
    37741 tttgctgttg atacagtaga tgcgaagaaa gtagtatctc cagaatttaa aaaagcagac
    37801 tcaaaagtag taatgttatg tgtaaacaag gcagaaaatg atgttgtaga ttttgaggaa
    37861 ttaaagagaa acttagacaa agtaagagaa cttatacatg gaaataaagt tttatcaaca
    37921 tatgcattag gatttgcagg tgtaggtgaa gcaataagta aaatggcttt tggtaataaa
    37981 ataggattta agtttagtga agaagctgaa aaaacattta cagatgataa attatttgag
    38041 gctagttatg gaaatatagt acttgaatta gcaaatgatg atttatgtat gttagaagga
    38101 tacaattatg ttgtgcttgg aagtacaact aaagaagcta gtatatttat caaaggtgaa
    38161 gaacttgcat tagatgaatt atataaagca cactgtagta ctttagagcc tatattccca
    38221 actaaaacag aagaagtaaa atcaaagata gagactataa gttatatatc acaaggagaa
    38281 gctaaaaaat catcattaag tatagctact ccaagagtat ttataccagc atttcctgga
    38341 acaaactgtg aatatgactc tgcaagagca tttgaaagag taggagctaa tgcaaacata
    38401 agagtattca agaatttaac ttataaagac atagaagatt ctatagatac aatagtaaac
    38461 gaaataaaat cttctcaaat aataatgtta ccaggtggat ttagtgctgg tgatgagcca
    38521 gatggttctg gtaaatttat agctactgta tttagaaatc ctagagtgca agaagctata
    38581 aatgaatttt taactcaaaa agatggacta atgttaggta tctgtaatgg attccaagta
    38641 ttaattaaat taggattagt tccatatgga gaaataagag ttccaagtga aagtgctcca
    38701 acacttactt acaataatat tggtcgtcat caagctaaga ttgctagaac tagaatttca
    38761 tctaataaat ctccttggtt agctcaaact aatgttggag atattcataa tatagcaatt
    38821 tctcatggag aaggtaaatt tgttgctagt gaagatgtta tgagagaatt aattgctaat
    38881 ggacaagtag caactcaata tgttgacttt aataatgaag ctacttatga tattgagttt
    38941 aatccaaatg gttcattcta tgctgttgaa ggtataacta gtgctgatgg tagagtgttt
    39001 ggtaaaatgg gacattctga gagaattggt gaggaagtat ttaagaatat tataggtgaa
    39061 aaagaccaaa agatatttga atctggagtt aaatatttta gataaagctt tgatgtttaa
    39121 ttgatagcta gaatatataa tttagatatt tagatttaaa gagatgtaat aatatgatga
    39181 taatcattta atattacatc tcttttttat ttatctaaga aataataagt tcaacctaag
    39241 ttaaaataca agctgtgtta taattaatta ctaagtaaaa ttaaatattt attggagggc
    39301 aacataatta atgaaaatat taataacagg gtctaatggt caattaggaa aagaattggt
    39361 tagccaatta gaagcaatca atcaaagtat aaatcaacca aaatacgtta tattagctac
    39421 aacaagaaat gatttggata tatccaatca gactaatata gataatttta ttttgcataa
    39481 taaacctgat gtaattgtaa actgtgcagc atatacaaag gttgatgcat gtgaagataa
    39541 tatcgaaatt gcgtataaaa taaatgcctt aggagtgaga aatttagcaa tagcatcaga
    39601 aaaagttaat gctaagcttg ttcacatatc aactgactat gtattcaatg gtttttctaa
    39661 atatccatat agggaagaca ataaaactga acccaattct gtgtatggaa aaagtaaact
    39721 tatgggagaa aagtttgttg aacaattctc acataagtac tttatattga gaacagcatg
    39781 gttatatggt aatgggaata attttgtaaa gactatgata aaactttctt tagaaaataa
    39841 agaggttaat gtggttaatg accagtttgg aaccccaaca agtacagtag acttagcaaa
    39901 aattataata agaatcatgg aaacagaaca ttatggagtt tatcatgcta catgtgaagg
    39961 tgagtgtagt tggtatgatt ttgccaaaaa gatatttgaa ttaaaaaata tagatataaa
    40021 agtaaatcca attaagagta acgaatttaa atgtaaggct caaagaccac tatattctgt
    40081 attggataat tttatgttga agttaattgg tttaaattct tttagaaagt gggaagaatc
    40141 cattgaagaa tatttagaaa gatatgatat aaatgaaaag taaatgtatt atttttagaa
    40201 aagaaaataa gatattataa aaaatataaa tatttctttt atttatccga taatcataat
    40261 atgacaaaaa caaatttaag gatggaaaaa gatgaaagga attattttag caggagggtc
    40321 tggtacaaga ctatatccta taacaaaatg tatatcaaaa caaacacttc caatatacga
    40381 taaaccaatg atatattacc ctatgtcggt tcttatgttg gcagggatac aagatatatt
    40441 aataatatct acaccaagag atataaaagc atttgaagaa ttatttggag atggaagttc
    40501 actagggctt aaaataacat atgcaattca ggagaaagca aatggaattg cagaagcgtt
    40561 tataatagga gaagaattta taggaaatga cagagtgtct cttgtattgg gggataatat
    40621 attttatgga tatggattta gtgaaaggtt ggagaatgca gttaaaagaa aaggagcaac
    40681 tatatttggg tatcatgtaa gtaatccaaa ttcgtttgga gttgtagaat ttgataagga
    40741 ttttaatgta ttatcaatag aagaaaaacc tacagttcct aaatctaatt atgctatacc
    40801 aggattatat ttttatgata atgatgttgt agagatagca aagctagttg aaccatcaga
    40861 tagaggtgaa ttagaaataa cctctataaa cagtgaatat ctaaaacgag gaaaactaaa
    40921 agtggaactt ctaggaagag gtatggcttg gcttgatact ggtacacata aaggtctatt
    40981 ggatgcttct aactatgttg aagtggttca aacaagacag ggactgtata tagcatgttt
    41041 agaagagatt gcatatacaa aaggatatat aagtagagaa caattaatga aactagccaa
    41101 gccactgata aaaactgaat atggtcaata tctattaaaa ttggctagtg aagatatgca
    41161 atatggtaga taaaatttat tataaatttt attaagggag tttgttataa tgggaaaaat
    41221 caatgttata aagactaaaa tagaaggatt atatatagtc gaaccaaatg tattttgtga
    41281 tgaaagagga tattttatgg aaagctataa taaaaaggat tttatagagc agggtcttga
    41341 tatgtgtttt attcaagata atgagtctag ctcaagaaaa ggtgttttaa gaggaatgca
    41401 ttttcaggtt agatatcctc aaggaaaatt agtcagagtt acagagggag aagttttcga
    41461 tgttgcagtt gatttaagaa aagattcaca gacatttggg aaatgggaag gtgtaatatt
    41521 gtctgataag aataaaaaac aattttatat tccagaaggg tttgcacatg gatttttagt
    41581 tttatcagaa aaagcagtat ttaattacaa atgtacaaac tattattatc cagaatttga
    41641 gcatgggttt atatggaatg acaagcaaat atctattgag tggcctttaa aaggaattga
    41701 agaaatattt ttatcagaaa aggacaaaaa acaaaaaagt tttaaagata tttgtataag
    41761 acgttaataa aaatttatta gaattaacat ttaggagaaa aaatatgaaa aaaatactga
    41821 taactggagg agcaggattt attgggagta actttgtaca ttatatgctt aacaagtatg
    41881 tagattattt tattgttaac ctagacttgt tgacttatgc tggtaattta gagacattaa
    41941 tagaaataca agacagttcc aattataaat ttgttaaagg agatatttca aatagggaac
    42001 agatgtataa gctatttgaa gaagaaaggt ttgatatagt tgttaacttt gccgctgaat
    42061 ctcatgtaga taggtcagtt gagaatcctg atttatttat taaaacaaac attataggaa
    42121 cacaagtact tttagatgca tctataaaat atggagttaa aaggtatcat caaatatcta
    42181 cagacgaagt gtatggtgat ttaccaattg atagaaagga tttattcttt acagaacaat
    42241 ctcctatcaa tccaagttct ccatattcag cttctaaagc atcagcagat ttgttggtaa
    42301 gttcttatta tagaacatat ggattgttaa caactatatc taggtgttcc aacaattatg
    42361 ggccatatca ttttcctgaa aaattgatac ctcttatgat tttaaatgct ttggaaaaca
    42421 aacaattgcc agtatatggg aatggggaaa atgtaagaga ttggttacat gtatatgacc
    42481 attgtactgc tatagactta ataatacata aagggaatat aggggagatt tacaatatag
    42541 gaggtcataa tgaaagaagt aatttagaag tagtaaaaat gatattaaat cttttagata
    42601 aatctgaaga gttaatatcc tatgtaaatg atagacctgg tcatgattta agatatgcta
    42661 ttgatgcaac taaaatagag aatgagttag gttggaaggc aaaatatgac tttgatttgg
    42721 gtataaaaga aactgttaag tggtatatag aaaatgaatc ttggtggaaa gctgttttat
    42781 cagtgtaact ttgaaattta taaaatttag aaaggggcgt tttatattga atgagataag
    42841 ttcactaatt aatatattag cattgactca attatctggt agtaatacta caaatcaatg
    42901 taactgtaca tataatagtt catctggatt tgatatgatt atgatgacct tgttaaaagc
    42961 attatcacaa aataatcaac agacttctaa tggctacaat ttatcgtcta attctgagaa
    43021 tttatttgac gaactcggca gtgtagtaaa taatacagct agtagattta tagatgttga
    43081 tacaaaagat aaaagtgtaa aatcaagaat tgaaaatgct atagagcaag catcaaaaaa
    43141 atataatgtg gattcgaatc ttataaaagc aataattaaa gttgaatcgg attttaatcc
    43201 aaatactgtt tcttctgctg gtgctaaggg tcttatgcaa cttatgccag aaaattgcag
    43261 agacttgggg gttacagacc cttttaatat agagcagaat atagatgctg gaactagaca
    43321 tattaaggaa tacattgata tgtttggagg gagcatagaa atgggattga tggcatataa
    43381 tggtggtcct ggaagaatga aaagtagagg agtagaatca atctctgacc tgtataaaat
    43441 gccaaaagaa acacaaaatt atattcctaa ggttatgaaa tattatagag ggtaggcttt
    43501 attatgggga taaatataga taagctaaac ataagtccaa gtataaatat ggaggttaaa
    43561 aaactaaaag agagtaataa attttctgac aaattaaact ctttaaaaca atcttcttca
    43621 ttggaagata taaaacgaaa tttggaaagt gtaaaaaagg caggagaaag gcttgtactt
    43681 actcaaaact atggcgacat tagtatatat aaaaatgcag taaaagaata cctaaaatct
    43741 gttgtagata atatgtattc acttgataaa aatagtagct tttgggagca tcagtattat
    43801 aagaatgtaa agattgttga tagtaaactt gaggatatga ctagtaagtt aattagtgaa
    43861 gaaaaagaaa acatagatat tgtttctact gttgatatga tacaaggctt gttgatagat
    43921 atgtatagat aaggttatac aaggagaaaa gttttatgaa tagtaaaaaa gaggatatat
    43981 atgaattgaa ttttgggcaa ttaatagaag aaaaatctgg tgttatagaa gaaaatcaaa
    44041 atgtttttaa tgcaaaagta aatgtagctg tttctatagg aaatacaaca gaaacaataa
    44101 aaaatatatt agatttaaaa gaaggctcta taataaaact aagtaagaat gtggatgaaa
    44161 aattagatat atatgcaaat gatagattgt ttgcttatgg agaaagcatt attacaaatg
    44221 agaagttatc tgtaagatta tctaaaatag aagattcaga tgtaaattag tattttaaat
    44281 agtatccctt taggctatga tgtatgagtt tagctaaggg gatattttta taaaaaattt
    44341 aaaatatgcg tgaattatat aaatgttttt attttatgag cgataattaa attataagta
    44401 tgtaattttg gaggtgaaac tatggatatt aaaaatgtaa gttcaaatat tgtagtgaat
    44461 aattatttaa agaatacatc tggtgtaaaa aaagtggaag aagttaaaaa aattaataaa
    44521 acagacaaga tagagctatc taaatttgca aaggaaatgg caagtgtgtc aaatatgagt
    44581 gatgagaata ttaggaataa aaaagtcgat gaaataaaaa atgcattaga aaatggaact
    44641 tacaaaataa aaccaaaaga aattgcgaaa aaaatgatag aggatatgcg aggataaagt
    44701 catgattaca gacttaaaag ttataatata tgaagaaaaa aatataatta gtaagatgat
    44761 ggaattatta aaagaacaat ttgattatat agttgaaaaa gacttagaaa atttaaatat
    44821 gttaaatcct aaactagaag atattagcag ggagcttgca tccatagaaa taaagagaag
    44881 acaacttttt ggtgatgata tttcaatgag tgaagttgtt gaaaatagta atgatagata
    44941 tttaaaagaa atatatacag acttgaaaca cattcttgct ttagctaaaa atcaacaaga
    45001 atcaaatgat tctctaataa agaaagaact tatttttacg aaaaaaatga ttaatttcat
    45061 aaaaccagct gataagcaaa ctgcaactta taattcttat ggaaatatta gaaaataaat
    45121 attaggagga gaaatatggc aggattattt tccattttaa atacagctaa ttcaggaatg
    45181 aatgcgcacc aaaaagcaat acagacaatt tctcataata taagtaatct agatacagac
    45241 ggttattcta ggcaaaggac tgagtttgct acaaatagtc caatgtatat gcctagtttg
    45301 agtaattcaa ttgcaagagg acaattaggg actggagtac acgtaacgga tgtaacaaga
    45361 gctagaaata gtttttatga ttaccaattt agagcagaag ctcataagta tggtaataca
    45421 gtttcaaagt atgactatta taatactata gaaactataa taaatgaacc atcagactat
    45481 ggaatatctg caggtataga cgactttttt aaaggatggg aatcattagc aaaggatata
    45541 aatagtgata gtaaaaaaag tctagtggta gaaaatgcat caaatctagc aactttaata
    45601 agtgaaagtt ataagaagct taataagtta aagggagata tagacgataa tataaactct
    45661 gaaataaaac agataaatga catgttaaaa agtcttgaag atttgaataa atctattgat
    45721 gttatatctg gttctggctc tactccaaat gatttacttg atgaaagaga tagaatatta
    45781 gatgacttaa gctttaaatt agaccttgaa aatagcgatg ttaaaaatat gctttcagat
    45841 ggtaaattag aacttaatga attaaaaaat gcagatggaa cttggaaaac aggtgtatca
    45901 ggaaccttac aaggcttgtt tgaaatgcat ggtaagattg atacatataa aagcgattta
    45961 aaagatgtat ctgatgggtt agctaagcag ataaatgatg tttataatac atctgctgga
    46021 ataacagtaa cagatttttt tataacttca aatgtagatg gtgaagatat cataaaggta
    46081 aatccagcta taaagagtaa cccaaatgaa ttaaaactta ctgctgatga ggcttcaaag
    46141 attgctaagt taaaggatga aaaaatagat attggggtgg ctggtggaaa agtaagtaca
    46201 ataagtgacc actataaagc ctttgcagaa tcagtaggtt tagattctca aaaagtgaat
    46261 caagatgaag tgaatcaacg taaaattata aataatattg ataactcacg aatgagtgtt
    46321 tcaggcgtat ctttggatga agaaatgaca gagcttataa aaatacaacg ctcctatcaa
    46381 gctagtgcaa aagttatgtc aacagctgtg caactgttgg atgttgtaat aaatggaata
    46441 atttaataga agggggaatt tataatgaga gttagtacag gtatgatgtc tagcaactat
    46501 ctaaactcac tacaagataa tctacaaaga ttagataagg ttaatagaca agttaataca
    46561 acgaaagaaa taaacaagct atcagataat ccatataagg caataaaaat attgaactca
    46621 aaaagtgaaa taaaaactat ggagacttat gttgagaatt gtaaagatac agggaattgg
    46681 ttagaaacta ctgatactgc attagatcag cttggaaaat tattatcgga cataaagaaa
    46741 actttggtgg caagtggtga tggtgcatat tcagatgatg agctaaagac tcttagtaat
    46801 gaaactaatg aaaagatgaa agaaattgca aatgccttaa atgcaacaca tgaaggaaag
    46861 tacatatttt caggttcaaa tacaggtgta ccacctgttg aatgtataga taatgctgac
    46921 ggttcagtga gcttaaagtt taattctagt ttaaatctta ataaattaaa tgactctttg
    46981 agtatggata tagctcaagg aataagtgta gattataatg ttaaattgag tagccttgga
    47041 tttgacccaa caaagactga cccatttgaa gagttaaata atatatcaaa gaaattagca
    47101 aatccagatg ctgatgctat aaaagagctg acaacaactt gtttaggcga catggaacaa
    47161 ttaatagaaa atacagtaaa tgttaggtct atatatggga caaaagcaaa tacagtagag
    47221 gctatgaagg aaaaaaatga tgatagtttg atacagttaa aggatatttt gtcacaaaat
    47281 gaagaaatag attatggaga aaaactcgtt caattaaagg cagcagagtt aacttatcaa
    47341 gcttcactac aaacaggagg aaagttattt aatgtatcta tattagatta catatagtaa
    47401 aataacgcag atatgatgaa ggttacatta aaaaaaggta tattaggttt tgaaaattta
    47461 aaagaatatg agcttttaga tatagaagat aatgattctt taaaagaatt taattctact
    47521 gaagaagaag gtataggatt tgttgttgta tctccatttg agattatcaa tgaatatgaa
    47581 atagttttaa atcaggaaac aatagaaaaa ctagaagtga aatctccaaa ggatgttgta
    47641 ctatttaata taattactct tggtcagaca tttgaagaat caactgtgaa catgaaagct
    47701 cctatagtga taaatataaa aactaatttt ggaatgcaaa taattttaca ggatgataaa
    47761 tattcaataa ggcatccatt attgagaggt gataatggat gctagtaatt tcaagaaaaa
    47821 aagatgaagc agtacttata ggagaaagca tagaagtaaa aattgtaggg gtagatggaa
    47881 acaatgtgaa actagctata tcggcgccta acaatataag tatattaaga aaagaaatat
    47941 acgaaaaagt aaagagtgag aatataaaag ctacaaataa aaatataaaa attttaaagt
    48001 cattaaaata ataagcttgg aggaagtaat atatgtatgg agaaaatcct tataattctt
    48061 ataaacaaaa tgctgttttt atggcatcaa aggaacaatt gttacttatg cttgttgatg
    48121 gagcagtaaa atataccaaa atagccagag cagctatctt agataaaaat acacagaaag
    48181 cgcatagaga acttgtaaga gttcaggata tatttacaga actaatggtt acattagacc
    48241 aaaatgcagg acaatgggca aaggatatgt acaaagttta tgattttgta agatatgaac
    48301 tttcaagagc caatattaga aaagacatcc aggtaataga taatgtgttg cctgtaatag
    48361 aacaaattaa agacacatgg catgaagctg ataaaaaaag taaagaagaa agaagtaggt
    48421 acaaataatg gaattaaatt tagctgaatt agataagtta tcaaaagagg aattactatt
    48481 gatgcttgta gatggcacag taaaatatac caatatatcc aaagaagctc ttttaaataa
    48541 cgactatttg aaagcacata atgaacttat aagagttcaa aatatattta cagaactaat
    48601 gactacatta gaccaagatg caggtcaatg ggcaaaggat atgtataaag tttatgaatt
    48661 tataaaatct gaattagcaa tagctgatga aaataaagat attaaaataa ttgatgatat
    48721 attgccaata gtaaaacaaa ttagagatac atggcatgaa gtttacaatc aactaaaaat
    48781 ataattagat ggagggataa aggtatgtca agtataagtc ctataagagt tacaggtctt
    48841 tcaggaaatt ttgatatgga aggcataatc gaagctagta tgattagaga caaggaaaaa
    48901 gttgataaag caaaacaaga acaacaaatc gttaaatgga agcaagaaat atatagaaat
    48961 gttatacaag aatcaaaaga tctttatgat aaatatctaa gcgtaaattc tcctaatagt
    49021 atagtaagtg aaaaagcata ctcttctaca agaataacca gttctgatga aagtattata
    49081 gtagcaaaag gctcagctgg tgcagaaaaa ataaattatc aatttgcagt ttctcaaatg
    49141 gctgaaccag caaaatttac tattaaatta aattcaagtg aacctattgt tcgacagttc
    49201 cctccaaatg ccagtggagc tagttcttta actataggag atgtaaatat accaatatct
    49261 gaacaagata ctacaagtac tattgtaagt aagataaact ccctttgcgc agataatgat
    49321 ataaaggctt cttatagtga gatgacaggt gaattgatta tttcgagaaa acaaactggt
    49381 tcgtcatcag acattaattt aaaagtaatt ggaaatgaca atttagctca gcaaattgct
    49441 aatgataatg gtatcacatt tgcaaatgat gctagtggaa acaaagtggc aagtgtatat
    49501 ggaaaaaatc tagaagctga tgtaactgat gaacatggaa gagtaactca tataagtaaa
    49561 gaacaaaatt catttaatat agataatatt gactataatg taaattcaaa aggaactgca
    49621 aagttgactt ctgtcactga tactgaagaa gctgttaaaa atatgcaagc atttgtggat
    49681 gattataata aactgatgga caaggtctat ggtttagtta ctactaaaaa accaaaagat
    49741 tatccgcctc ttacagatgc ccaaaaagaa gatatgacaa ctgaagaaat agaaaaatgg
    49801 gaaaagaaag ctaaagaagg tatacttaga aatgatgatg agttaagagg ttttgttgaa
    49861 gatattcagt ctgcattttt tggagatgga aaaaatatta ttgcattaag aaaactaggt
    49921 atcaatgaaa gcgaaaatta caataaaaaa ggtcaaatat catttaatgc agatactttt
    49981 tcaaaggctc ttatagatga tagtgataag gtatacaaaa cactagcagg ttattcttcg
    50041 aattatgatg ataagggaat gtttgaaaag ctaaaagata ttgtatatga atattctgga
    50101 agttcaactt ctaaacttcc taaaaaagca ggtatagaaa aaactgcttc tgctagtgaa
    50161 aatgtatatt caaaacaaat tgcagagcaa gaaagaaata taagcaggtt agttgaaaaa
    50221 atgaatgata aagagaaaag actttatgct aaatattcag ccttagaatc tttgttgaat
    50281 cagtattctt cccaaatgaa ttatttctca caagcacagg gtaattaatt atggataaat
    50341 taaaagaaaa attaaattta tataaagata tatctttaca aattataaat ttgattgaaa
    50401 aggaagaata tataaagata agtagtaagt taggtgaaag acaagagatt ataaatagtg
    50461 taagtgaaat tgatagaaat gattttatac agctttataa tagaatggaa ttaattgaaa
    50521 ttgatagtag gataagagat attttacaag gacaattatt agaagtaaaa aaagaattac
    50581 atgagtataa gcttactaaa caagtgaaca caatgtatta taacttaaat agagaaaaag
    50641 taaatatttt taataaaaaa gtataaagtt atgtaccatc ttgtccgatt atataaatga
    50701 agaattaata aaaaagatta aaagtagaaa tgacaaggat gtcaactata ctaaggaggg
    50761 taaaataatg agagttaata caaatgtaag tgctttgata gcaaataacc aaatgggaag
    50821 aaacgtaaat gcacaaagta agtctatgga gaagttatct tctggtgtta gaattaagag
    50881 agctgctgat gatgctgctg ggcttgctat atctgagaaa atgagagctc aaataaaagg
    50941 tttagaccaa gcaggaagaa acgttcaaga tggtatatct gttgtacaaa cagcagaagg
    51001 tgcattagaa gaaactggta atatactaca aagaatgaga actttatcag tacaatcttc
    51061 aaatgaaact aatactgctg aagaaagaca aaagatagct gatgagttgt tacaattaaa
    51121 ggatgaagtt gaaagaatat caagctcaat tgagtttaat ggtaagaaat tacttgatgg
    51181 aagttctaca gaaataagat tacaagttgg agctaatttt ggaacaaatg ttgcaggaac
    51241 aactaacaac aacaatgaaa taaaagttgc tctagtaaat acttcaagta taatgagtaa
    51301 agcaggtata acaagttcta caatagcaag cttaaatgca gatggaactt ctggaacaga
    51361 tgcagctaaa caaatggtgt ctagcttaga tgtagctctt aaagaattaa atacatcaag
    51421 agcaaaatta ggggcacaac aaaatagact agaatcaact caaaacaact taaataatac
    51481 tatagaaaat gttacagcag ctgaatcaag aataagagat acagatgttg cttcagaaat
    51541 ggttaactta tctaaaatga atatattagt tcaagcatca cagtcaatgc ttgctcaagc
    51601 taatcagcaa ccacaaggag ttttacaatt attaggataa aataattttg tatattggat
    51661 atagaagaaa gggtaaggct ttgctttact cctttctttt tttacaaata tacttttgaa
    51721 aggaaaatat atatgttact tagtatagtt atgattgtta aaaatgaaga aaaaatactt
    51781 gaaaaaactt taaaatcatt aaccacatta agaagttcta tagagagtga acttataata
    51841 gttgatacag gttctacaga tgatactata aatatatcta ggaaatacac ggaaaatgtg
    51901 tactttcata attggaataa tgatttttct agtatgagaa atatatcaat atcctatgct
    51961 aaaggtgaat ggttacttat attagatgca gatgaaatac taattgattc ttcaactatt
    52021 gtgaaatttt ttaaagatgg tcttgataaa aaatttaatt cggcctcaat acaattaaaa
    52081 aatctttact catatgataa aaaattatat ggatattgct cagtgcttag actttttaaa
    52141 aacatcgatt ttagatacca tggtaaagta catgaacaac caatatacaa aaaccctata
    52201 tttaataatg ttgctgattt tgaacattat ggatatttgt ttgaagatga agaaataaga
    52261 ttaaataaag taaaaagaaa tgaaaaactg ttatttgaag aattaaaaga gaatgataat
    52321 tctccatata caaattatca attagggaaa aattttatta tattaggaaa atatcatgag
    52381 gctttatact atcttgagaa atcttatgaa ttatattcta aactagaatc ggcacctggt
    52441 tacttgataa caagtcttgt aaaaacatat ttatatttag gaaagcataa gaagtgtgaa
    52501 aaattgtgtc taaagtatat taagaaagac atgaataata ttgatatata ttattatatt
    52561 gcacaagcac aagtagattt agggaagtat gaaaatagta tagattcgta taagagatat
    52621 atttatttgt tagataatta tgaaataagt acacaagcaa atagtttatt ttcagacact
    52681 gatactgtag ggcttaggga tgaagctatt attacactga taaagattta ttataaactt
    52741 gaaaaatatg agtctgtaat ttcagagtac aataacattg aggatatgga aaagaagaag
    52801 aatgtgtact ttagcttatt tatgtcccta tacaaattga acagatttga agatataaaa
    52861 aactattata aagaagtttc aaattctaaa attgaaaaaa atagttttta taaaaatata
    52921 gaagaagtta taaataatat taaagatgat gaaaaagagc atgtatataa aatcctttgt
    52981 gacattgaag gtaattatgg cattttaaat cagataagga taaataaatg tgtatctata
    53041 gataagtgta aagaaatact caatgaagaa aaagaaataa tttatgcata tattataaaa
    53101 gtagcttttg aagataagat tgatttacta gatatttttt atgaaatgga atatgtatgg
    53161 atagagaaat acctaaaata tcttttagca ctagaaagaa gttttaattt agtactatat
    53221 aagtacataa ttagtcgtcc gaatacatca gatataaaaa agataagagt atataaagct
    53281 atatctaaag tagttttaga aagtataagt ttaagtgatg aaaaatataa ggagattttt
    53341 tatctttata taatgtattc ttataagtac ataagataca tatatagaaa tttaagcgat
    53401 tttgaattaa taaaatttgt ttgcaatgat ttagataagt ttactataga atttaagcat
    53461 ctacttgaca ttaaaaataa agtaaatgat gctaatgaaa aattagagta tatacatagt
    53521 atgaggaaat tactaaatga gtatcctttt tataataaaa taataaaaat acttataagt
    53581 gaattagaag aaaatataaa agaaagtgaa gaatttaagg atttaaaaga tagttttatt
    53641 attagtatag aaaatatgat tgaaactggc aaaataaatg atgcgaaatc attggtagat
    53701 gactattcaa aaacatttat ggatgatgta aaaattttaa atataaaggg aattttatat
    53761 atgtttgaaa ataagcttga taaagctgat tttatgttaa aaaaggcatt atcattagat
    53821 gtagaaaatg aagatactat atataatata gaatacttaa aaagtttaag ataagtcaaa
    53881 aatgggagaa ttgaagaatg aatacaccaa tagtggttaa tgaaaaatct aaagatataa
    53941 taaaagattg gcaatatacg tatacaaacg aagatataaa atttacaagc ataataatat
    54001 tgacatataa tcaaatagaa tatactaaat tatgtattga aagtattagg aagtttaccc
    54061 caaagaataa atatgaaata ataataattg acaataactc ttctgatgga acagttgagt
    54121 ggttaaaggg acaatctgac ataaaatctg tatataataa caaaaaccta ggatttccaa
    54181 aaggatgcaa tcaaggtatt gagttggcta ctggtgaaaa catattgttg ttaaataatg
    54241 atgtaatagt tactccaaat tggctttata atttgaataa agctttatgg tctcaagata
    54301 atataggtgc tgtaggtcct attacaaatg catgttcgta ctatcaacaa attgatgttg
    54361 attataaatc aattgaggat atgtttatgt ttgcacacaa aataaataca tctaatatta
    54421 acctatggaa atatagatta aagttagttg gattttgcat gttaataaaa aaatctgtgc
    54481 tggataatgt aggggtttta gatgaattat tcacacctgg aaattttgaa gatgatgatt
    54541 tatcttttag gatgctagag tcaggatata agctattatt atgccaggat acttttatac
    54601 accattttgg aagtgtatct tttaaagaaa atcgtaataa atatacagat ataatgtata
    54661 ttaatagaag aaaatttgaa actaagtggg gatttaatcc agaatatagt ggaatgataa
    54721 gaacagattt gatttctaaa atagatgata gtttggaggc aaatattaat gttttagaaa
    54781 taggctgtgg tataggggct actcttttag aaataaaaaa tatatataaa aatgcatcaa
    54841 tatatggaat agaaattagt gagtcagcag gtaaacttgc aaagtatagt gcagatgtac
    54901 ttatagcaga tatagaaaaa gtaaagttgg attatgaaaa agattttttt gattatataa
    54961 tattgggaga tgtacttgaa catttaactg acccatggaa gatagtcaat gaactgaaac
    55021 aatatctaaa gaaggatggt tgtttgatat ctagtatacc aaatattatg catgtttctg
    55081 ttttaagaaa ccttgtgtta ggtaatttta catatgaaga ttcaggaatt ttagacaaga
    55141 ctcatttaag gttttttaca ttaaatgaga taacaaaaat gtttaatgat agtaattaca
    55201 taataaatag tgttacagct actagtatgc cactttcgga aattgataat agattcattg
    55261 atacaatgtg taaggaaagc tgtaaagaaa tgagacaaca gtacgaatct tatcaatata
    55321 tagtaaaagc cactaaaagc atagatacta aaagatattc tagtattagt atggttaatt
    55381 taaaatacgc tttaatgaga attgataatg aattagatgt agatagtaat ttaagttata
    55441 tatttgataa ttataatata actgaaccat gttttgttga tgatattgaa tatttagttg
    55501 atacgtttgt gataaataaa gcaactctat taaatagatt aggagttgag gcatttaaca
    55561 gaggtttagg agattttagt ataaacatgt ttatgacagt attagagatg gatagagatg
    55621 atattgattc aatatataat ataacaagtg tattatgtga attagaagaa tttgatactg
    55681 cttacaactt aattaaaaat agtagcaaaa aagtacagag tgaaagtgac atacaagata
    55741 tactttattt tattgagggg aagagatatg aataatgata agatatgttt tataacctgt
    55801 gtaaatgatg aaagacttta tgaggaatgt cttgtatata taaatagcct agacattcct
    55861 aaaaacttta gcatagaaac agtagcttta agaggagccg attctattgc ctctgcttat
    55921 aataaagcta tgagagaaac agattctaag tataaggtat acttacatca agatgctttt
    55981 ataattaata aaaatttcat atatgacatt ttagagatat ttaaaaaaaa tacagatgta
    56041 ggaatgattg gtgtagctgg agtaaaagat atacctgtga atggaatttg gtgggattct
    56101 gataaaaaag cagggaaagt atatgatagc catacaggta aaatggatat ttatgaattt
    56161 agtaactttg aagaatgttt taatgaagtt atggctatag atggattaat tatgattact
    56221 caatatgatg tattttggag agaggataag tttgatgggt ggcattttta tgatttgtca
    56281 cagagtatgg aatttagaca aaataaatac aaagttattg taccaaaaca aaataagcct
    56341 tggtgtatgc atgattctgg agtagcaaat ataaataatg gatatgatga atataggata
    56401 aaatttgtaa aagagtacat aaatcttgat ttatatagag aaaattttta tgaatttgat
    56461 aagaagaaag caagtaccat acttaagagt aataagcatg taatggattg tatgaaagag
    56521 gagcttacag aacaattaag attaaagaat tataaggatg ttttagatat tgtttctaaa
    56581 attgctcatt tttctgtttt taatcatact ggcttgtatt cttgtgaaaa atgtgagaga
    56641 gcattgctag aatgtgcaaa taatttacca gaaatagaaa atacgaaatt atttacaaaa
    56701 aaagatttaa aaagaagaaa agtattacat gttttaagtg aaggatattc aacaggaggg
    56761 catacaagac tagttaaaaa ttggattgag attgacaaag attctgttca ttcagttgta
    56821 actacatggc aatcagatac actgccagac tggttattaa aagcaataga tgatagtggt
    56881 ggcgaagtta tatctttgga tttaatttca gatgattatt taaaaagagc tgtagctttg
    56941 aggaaaatag cttatgactg ggctgatata gttattttat atgttcatat gtgggataca
    57001 atacctgttg ttgcttttgg agttgatggg aaaataccta ttttacatgt taatcactat
    57061 gatagtactt tttggttagg gacaagtatt gcagatattg tcattgattt tagcgaagct
    57121 agtcaattaa ttacattaga taaaagaggc atagataaat ctgaaatatt accaatgcca
    57181 ttaaaagaaa aagaagtatt ttcaaaatct gaagttagaa aacgatataa tttggaggat
    57241 gatgtgacaa taatattgac tattgctagt gaaagaaaat acaatagtgt aaatgatatt
    57301 aattacttta aaattgctaa aacattgatt gaaaatacag ataatacaat tattattgtt
    57361 gtaggagttg atacagagaa tctctattgg aaaactttaa gtaacgttac agataataga
    57421 gttatatgtt ttggaacaac agcaaatata gacgattttt ataagatttc ggatatatac
    57481 ctagagtctt tcatggtagg gagtcatact tcaaaattag atgcaatttt atatgattta
    57541 attcctgtta agtttaaaaa tgaagtttca ccaattttta caaatatgtg gaattttata
    57601 gataagtttg attataagaa tataaaagag gttatagaca tgataagaag gtacaatact
    57661 agtgaatttg acagggataa gataaaagaa aaacaatcag aaataaaaca ttttgttata
    57721 gataaacact gtattgaaat aaaaaactat atagacaaaa tatataaaga ggctaatcat
    57781 cataaagtta agtatgattt atataaaaat gatgaaatcg aagactatca gctttttgtt
    57841 gcattatatg gttatatggt aaaaaataaa aacatgtcat aggtgataaa atgaacaata
    57901 ttcatatcaa taaatttgaa aaaagctgtg aaaaaaactg ggattatttt gtagaaaata
    57961 aatctataaa tggtacattt ctgcaaacta gaaatttcct taattatcat gaagaaggaa
    58021 aattcactga ccactcatta ataatatata aaggtgaaga aatactagca gtcgtacctg
    58081 catgtgaaac ttatgaaaat ggtgaaagag tatttttttc tcatttagga agcacttttg
    58141 gaggtattat aataagaagt ggttttaatg atattaggca tgtaaatgct attacaaata
    58201 agttagaaac atatttacag gaacaaagtt tcaataaagt atatttaaaa aatacatcaa
    58261 gaatattttg taaagaggat acttcattac tagaatattt tctatttaag aataattaca
    58321 tgttttatga tgaattaagt atgtttttag aattaagtaa gtataaaaaa gatatattag
    58381 ctaattttag atatagtaaa agaagatatt ttaaatattc tttgaagaat aatcttacct
    58441 ttaaaagatt gtatagtgat gaggatatag tttgtttttt taatttactt tctaattgtt
    58501 tagatagtaa aggagctaaa gtaatacatt cagtagaaga acttttggag tttaaaaata
    58561 atagattaaa agatattgta gaattttatg cagtatatat tgatgacatt atgatttgtg
    58621 ggagcatggt atttaacttt caagaaagag tttatcatac tcaatatcta gcatcaaatt
    58681 cgacatataa taaatattat ccaatgatat ttttatacta taatctaata aaagatgctt
    58741 tagagagaaa ttttaagtat ctttcttttg gaattagcac tctaaataga ggcaaatact
    58801 tagacgaggg tttagctaca tttaaagaag gttttggttc aagtgcaatt tgcaacagaa
    58861 catactataa aaatataaat aagtgataat catgttttat agagttttta gagtatagta
    58921 aatatctagt actctaatat tacataaaaa taattagttt ataaagaagg tttgtatatg
    58981 aaaagattaa tgattttagg ctcaattaag tattttgaga atattgttat gtcagctaaa
    59041 aaagagggaa tatacacaat tgtttgtgat aatagaattg atactcctgc gaaaaagatt
    59101 tgtgatgaag caatagatgt tgatgtattt gattttgata aaataaaaag tatagctata
    59161 gataaaaaga tagatggaat tttaactgga tttacagatt ctttaatgaa accatatgtt
    59221 tatattgcaa atgaactaaa tttaccatgt gtgatttctt gcaatcaact tgaatctgta
    59281 actaataagg ctattatgaa aaaatatttt aaaaataatg caatacctac aactgattat
    59341 tgtattattg aaagtctaaa agatttagaa aaaatacaag gattaaattt tccacttgta
    59401 ataaaacctg ttgatggtta tggttcaaga ggtgtatatt ttgtatcaaa tatagaagaa
    59461 ttaatagaaa aatttgaata ttctaaggaa gcctcacaag atggacaagt aataattgag
    59521 gaattttatg aaagtgagga aatacaagga ctagcttggg ttcataatgg agagtcacat
    59581 gtattttata ttggagatag agagcttgta aatatacata caggaagagc tggaaaacca
    59641 gatagattac tttatccatc aaagtattgt tttcaatatg aggaagaaat aaaatctata
    59701 tatcaaaata taacacaggc ttttgatata aaaaatggac ctctttatgt acaaatgtta
    59761 gtgggagcat atggaataaa ggtatctgaa gtaatgccaa gattacctgg aggttgtgac
    59821 tatatggcta tttctcaaat aacagattta gacataggaa gtttatttgc aaactttagt
    59881 gtgaataatg aagtaaattt tgatgaaata aagaaatata atatgcattt atcaaaatgt
    59941 gtatatgcat taccaatata cataagacct ggaacaataa aaaagataaa taatatagat
    60001 aaaatagaaa acttagatta tgtcactcaa tttctattat tagtaaaaga aggagataca
    60061 ataggattat atgaggatga gagacaggat tgtggaagag tttatggaat agcaaataat
    60121 atatttgatg caaatataaa gaagaaatgt attcatgaaa tgatagaaat atttgatgaa
    60181 aataatgaaa atatgataga aaatttttaa ataagggagt atgatagata tgaagctaat
    60241 aactcatgaa caaattatgt cattaaacat ttctcagaaa caaggatatg aatggataaa
    60301 agaatctttt ttaacaaaaa aagattgtca attaccacca aaaataagca tggtagaaga
    60361 aggtcatgta ttttacaatg taatgccgtc cttaaatcat aaccatatga ttggtggaat
    60421 aaaaatggtt acaagattgc cagggagaaa tccaagttta aattcagata tatttctata
    60481 caatatgaaa gatgctaaat taaaggctat tttggatggt aattatatta cagcttttag
    60541 aactgctgct gttgctggtt tggctattga tacttttgct aaaaaagatt ttaatactat
    60601 ttctattatg ggtttaggtg taactgcttc tgctataatg aatatgcttt cgtatactct
    60661 taaagataaa aatataaata taaagttact taaatataaa gaccaagcag agtcatttat
    60721 aaaaaagtat gaaaaaaatg aacaatttag ttttgaaata tgtgatacac atgaagaact
    60781 tattgtagat tcagatgtag ttatttctac tgtgacatat acagatgaat atttatcaga
    60841 atttaaatgg tttaaaaaag gtgctttatt attacctgta catgttatgg gatttggaaa
    60901 tgttgattat ctatttgata aagtatattt agatgattat aatcatttga aacatctaga
    60961 aaatatagat agatataaat attcttgtga attttataat gttttaagta aacaagctaa
    61021 aggaagagaa aatgatgatg agagaataat atcttacaat ataggtttgt caatacatga
    61081 tatattattt gcaaacaaaa tttacgaatt gtgtgaaaca ctaaattgtg aagagataga
    61141 attaaaagaa ccaaaagaaa aatattggat atagtttatt tcattcatat ttacaataat
    61201 ttattgtaaa tatgaatatg aagtaatata ttgttagaaa attagcatga ataggataga
    61261 taaatttatg gataaatata atttaattaa gttcttatct attggaaacg aatatatagg
    61321 aaatttagtt gcattagaat caaataaagc aatacctttt gatataaaga gagtttatta
    61381 catatatgat gtaccaaaca atgtaaagag aggatttcat gcacacaaaa acttggaaca
    61441 agtactagtt tgcctaaatg gaagtgtgaa gataaaatgt tatgatggtg taaaagagag
    61501 tatatttaat ttacataaaa atgatgttgg attgtatata ggaacgggag tgtggcatga
    61561 gatgtttgac tttgatgaaa aaactgtttt aatttctata gcatctgaac attacaatga
    61621 agaagattat attagagact ataaagaatt tttaaagtat ttaaataata tgtagttatt
    61681 atacagttag tgatgtattt tcttaattaa ataaatttaa gatagccata tagagaataa
    61741 atagattagg atgtgaattt attgttggtt aaatttaata atttattaaa acagttccaa
    61801 actcataaag aagaatatga acagaagatt aaggatgtaa tggaaagtgg atattatatc
    61861 cttggaaatg aagtaaggca ttttgaagaa gaatttgcta gctatgtagg tagcaaatat
    61921 tgtgttggtt tagccagtgg tttagatgca cttataattg cttttagagc attaggaata
    61981 agtgaaggtg atgaagtaat cgtgcaatca aatacatata ttgctagtgt tatgggaatc
    62041 agcataaatg gagcaacacc agtatttgta gaaccaaatg aatattataa tatagattca
    62101 tctaagatag aagaaaagat aaatagcaaa acaaaagcta tattagtagt tcatttatat
    62161 ggtcaagcaa gtaaaatgaa agatatacaa gaattatgtg ataagtataa cttgtattta
    62221 attgaagatt gtgctcaatc tcatggagct aaatatgatg gaaaaatgac aggaacattt
    62281 ggtgatatag gttgttttag tttttatcca agtaaaaatt tgggtgcatt tggagatgca
    62341 ggagctatag taactagtaa tgaggaaata gctgataaag tgaaaattct tagaaactat
    62401 ggttcagaaa agcgatatta taataaattt gtcggatata attcaagatt ggacgaaatt
    62461 caggcaggtt tattaagagt aaaacttaaa tatctaaatg aaattgaaat tgaaaaatca
    62521 aaaatcgtag atagatatct taatgaaatt aataatccca aagtcatact tccaaagata
    62581 gaaaataatt gtactcatgt atggcactta tttgttttac aagttgaaaa acgagataca
    62641 tttatggaat acttaaagaa aaatggtata gagtctataa tacattatcc gataccacca
    62701 catctatctg aagcatatgc ttatttggga tataaaaagg ggagttttaa tatagctgag
    62761 aactattcag ataaagttgt tagtatacct ttatttgtcg gtatggaaga tgaagaagtt
    62821 agttatataa ttaaaattat aaatgaatat taaactaatc ataataatat caaaaataaa
    62881 ttttctatat atcatttatt taaatttcgt taattgacat ttttaaatat tgatatattg
    62941 tacaaataaa attgaaatat atggaagaat atagaataaa gatgagattt gtctaatatt
    63001 tttataaatt aaaaatattt tcaaagagtt tattataaaa ttgtagacta tagtgtttat
    63061 aatctttgta attattggta actaaattta aaaaataaaa tatttaatta tacacaaaaa
    63121 agatatttaa aaaatttgtt atttattgta tacttaagtt aaaactaaat aggcaaatct
    63181 agagaaatct agtgacgcaa agctataggg gctaaggttt gtatacacaa actatgtcag
    63241 ccagttgcca aaaagagttc taggtatatt gtatacgtag gaagagtcta ggatgactgc
    63301 ctttttggtg gtcattttgt tatggaaaaa atcacattat gtagtaaaaa acacctataa
    63361 taacatatta taccaaatat aatttaaaat aaagataaat tatctataaa agttactatt
    63421 ttacaaaaaa aggcaacttt ataaagaaat
//
LOCUS       DAMJWP010000005        56856 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.005, whole genome shotgun sequence.
ACCESSION   DAMJWP010000005 DAMJWP010000000
VERSION     DAMJWP010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 56856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56856
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.005"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(140..3475)
                     /locus_tag="PC700_000286"
     CDS             complement(140..3475)
                     /locus_tag="PC700_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDF2579614.1"
                     /translation="MGNFDFLQKKKNFNSFNTACLEAEKSILVSPSTCATITRRALEL
                     AVKWLYANDSDLVLPYQDNLSTLIHNNSFIELIDYDMLPLLKYIVKLGNLSVHTSANI
                     ERGEAILSLNNLHQFVSWIDYCYSDEYTAEDFNEDLLLHGEEKRTRPDELKNLYERLS
                     SKDKKLEEVIKENEKLRKSLTEKRKVNTVNYDFKIDEVNEFETRKRYINIELKLAGWE
                     FGKDIGEEIEVQGMPNESGVGYVDYVLYGENGKPLAVVEAKRTSKDPKIGQQQAKLYA
                     DCLEKRYSQRPVIFLTNGFDMYIWDDYSDRKVYGFYKKSELQLMIDRRKSKKSLSSVT
                     INDEISNRYYQKEAIRAVCEALENKQRKTLLVCATGTGKTRIAISIVDVLSRHNWIKN
                     ILFLADRKALVKQAKKSFTKLLPNLALCNLLDSKDSPEDARMIFSTYPTMMNAIDDTK
                     SKDGKRLFTPGHFDLIIIDESHRSIYKKYKSIFDYFDSYLMGLTATPKDEIDKNTYSV
                     FDMENGVPTYAYEYNKAVEDGYLVDYTSIEFKTKIMEDGIKYDELSDEEKEEYENTFN
                     DDESIGDEIGNNAVNEWLFNSDTIDLVLNKLMTEGLKIEGEEKIGKTIIFAKNTKHAR
                     AIVERFNKLYPKYGGNFVKAVDYSINYVDSIIDDFSDKNKLPQIAVSVDMLDTGIDIP
                     EILNLVFFKKVRSKTKFWQMIGRGTRLCPDLLGVDMDKERFLIFDFCNNFEFFKFNPK
                     GFEGNKAETLTEKLFNIKVSMVKELQDIKYIEGKYAELRKELLEELITSVKALNEDSY
                     IVRMNLSYVHKYKNENVWSNIGAVAQNEIREYISPLITSYSDDELAKRFDVVMYNIQL
                     AYLQNNNASKGIRHVMVTAEKLSKLGTIPQIQQQKYTIEKAMTEDFWEDSDIFEVEEV
                     RISLRELIKYLEKSSQKIYYTSFEDMIVAEDRNDSVYNANNLKNYKKKVEYYLNSHKD
                     ELAIFKLRNNKKITKQDVETLEEILLKQLGNCDDYKKEFGDTPVSQLVRKLVGLDREA
                     ANEAFSEFLNNKSFNTKQIHFVKLIVDYVVKNGFIEDNKVLMEDPFRTVGSIIDLFEN
                     HIEERNKLIKTINKIKENASEIG"
     gene            complement(3525..3953)
                     /locus_tag="PC700_000287"
     CDS             complement(3525..3953)
                     /locus_tag="PC700_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2579615.1"
                     /translation="MVIRNKLSWNSIEEVNRDFGSCLYDLQNFKILYDKEEMPELWAR
                     YIKEGFKSYMVSFLELTKAMLYYKSIDLNIKSKNFYDYLLACEYHNLLPKNSSIVIET
                     LRKLRNDDSHGYDIPQFEDMYELFTENEEVFVSIRNSCKK"
     gene            complement(3943..4323)
                     /locus_tag="PC700_000288"
     CDS             complement(3943..4323)
                     /locus_tag="PC700_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDF2579616.1"
                     /translation="MRNIPNEIKNSIINVFPKENTLAIIIFGSYGTDKHTSESDIDIA
                     WIPIKKVPITELATKTEALRNILDIDVDLKIVTDNYTIALKKAILEGEVIYESKDFKD
                     YINKFYFENSDVIDILDWRDMYGN"
     gene            complement(4356..4664)
                     /locus_tag="PC700_000289"
     CDS             complement(4356..4664)
                     /locus_tag="PC700_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2579617.1"
                     /translation="MNQIELDLYHDMVNIYKEADLQCNYKPTRFLQLINNKGALLAAK
                     ELINKEGATEGFTRLWECKRLDLSLEVLVLKEKYKELFTDEEREICINRLKDYGYKFN
                     "
     gene            complement(4697..5881)
                     /locus_tag="PC700_000290"
     CDS             complement(4697..5881)
                     /locus_tag="PC700_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDF2579618.1"
                     /translation="MVDEAKILDVVSISRENVKKFDGERSYLSTGNLDFNKISNLEIV
                     TYENKPSRANQTVNIGEVIFAKMKDTKKTLVINKTNKNIIVSTGFYVLKPSKEILPQY
                     LYHYLNSSYFLNQKNRLSKGATQSALNNEGLANIKIRMYNLKVQEKVVRVLDKAQELI
                     DKRKEQIEVLDELVKSRFIEMFGTPSKNEKNWEISEIGKYLDVLTDYHSNGSYETLRD
                     NVTLLDTKGYALMVRTTDLENNNFEKGVKYIDEHAYNYLEKSKVFGGEVIINKIGSAG
                     KVYLMPFLNKPVSLAMNQFMLRFNEDKVNHIFLYNLLLTSYMESKIKEKVRGAVTKTI
                     TKDAVRKINIIVPPIRLQNQFANFVKQVNSLKFEMETSLKELEDNFNSLMQKAFKGEL
                     FN"
     gene            complement(5885..7348)
                     /locus_tag="PC700_000291"
     CDS             complement(5885..7348)
                     /locus_tag="PC700_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HDF2579619.1"
                     /translation="MITGELRSKVDKIWETFWTGGLTNPLEVIEQFTYLLFIKGLDEV
                     ETKNEAEATLLGVSFEKIFDDEHQHLRWSKFSNEGNSEKMYEIVQNEVFPFIKKLHGN
                     KESAYAKYMGDAIFKIPTPLMLSKIVDGISNLELSKDRDTKGDLYEYLLSKVATAGTN
                     GQFRTPRHIIDMIVRLIKPTPEDIIVDPAAGSAGFLVSSQQYLRDNHSSLFLVQGLKE
                     HFNNGMFYGFDMDRTMLRIGAMNMMLHGVDNPNIEYKDSLSEVNTDKDKFTLVLANPP
                     FKGSLDYEAVSADLLKVTKTKKTELLFLALFLRVLKIGGRCACIVPDGVLFGSTGGHK
                     SIRKEIVDNHKLEAIISMPSGVFKPYAGVSTAIMIFTKTGTGGTDKVWFYDMKADGYS
                     LDDKRNEIKDSDIEDIIKRFDNLDGEVDRKRTEQSFFVDVEEIRENGYDLSINKYKEV
                     VYEEVVYDAPSVIIGRVKVLEKEIVDGLEELERMLEV"
     gene            complement(7858..8727)
                     /locus_tag="PC700_000292"
     CDS             complement(7858..8727)
                     /locus_tag="PC700_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HDF2579620.1"
                     /translation="MALVTTKQILLDAQEGHYAVGAFNVENMEMVMAVIEAAEELKSP
                     VILQTTPSTIKYAGLDYYLANVKVASEKASVPVAMHLDHGSSFGLAMQALRVGYTSIM
                     IDGSHESYEDNISISKSVVDACMPSDIPVEAELGKVGGKEDDLDGGDGGAYTDPLEAK
                     EFVERTGVSSLAVAIGTAHGLYKGEPKLDLDRLSEIREVVSVPLVLHGGSGIPDEVIK
                     ESIKRGICKVNYATELRIAYSNGVKDVLNSDPEVIDPKKYGKKGLESVKDFVKSRMEV
                     CGCVGKAIGTVKC"
     gene            complement(8747..10663)
                     /locus_tag="PC700_000293"
     CDS             complement(8747..10663)
                     /locus_tag="PC700_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HDF2579621.1"
                     /translation="MGKKMILGVTGCPTGIAHTFMAEEALKNSARELGCDIKVETNGA
                     IGVENKLTAKDIEMADAIIVACDKNVDMDRFNGKPVIEVPVKEGIHKANELIQRCIDG
                     KVAIRKGNLSSSKIAEEGNLSFGQKLYKDLMNGVSHMLPLVVAGGVLTAISFLWGIYS
                     FDPNSEQYNQIAATLKSVGGYSMNLMVPVLAAFIAQSISGRAGMLAGLVGGMISFDTG
                     SGFLGGIISGFLAGYLVKLFVHLLRKLPRQLEGLKSIFIIPIVSVGLVGVAMLLLGGP
                     CSALNNAMMNFLSGLQNSSPIILGIVIGCMSAFDMGGPVNKAAYVTGTMLLGQGNYLF
                     MAGVSAACITPPLVIAIASTLFKNRFTEEDRAAGLINYILGSTHITEGAIPFAAKNPL
                     KVLPVLMIASSISSILTYIMKIEVPAPHGGFLVLGLVNKPLLWVGCIFVGSLVGAVLY
                     MMVTPKVVNEKNTIENTNATKDGHKNESAESENVSNNISLYSEETVILDVKGKNKTDV
                     IDEMIGILDKSGVLLDKKKFKEEILKREEISSTGFGMGIAIPHAKTDAVKVPRVAVGV
                     SKEGFDFESEDGSLAHIIFMISATDNGDNLHLKTLSQLSSKLMNEEFLNELLNSKTSR
                     EIVSKLNNEEIKSV"
     gene            complement(10666..11586)
                     /locus_tag="PC700_000294"
     CDS             complement(10666..11586)
                     /locus_tag="PC700_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HDF2579622.1"
                     /translation="MIYTLTTNPAIDMNISTNGIKRKLVNRTSNAVYSPNGKGLNVTF
                     VLGHYGIESKILGFFGGFSGKYIVEESEKRGFDVLPTWVEDTTRINIFLNDGSDEFKF
                     VNSGSYVNEKQKLDMLDKIQSLADISYLSISGSLPPGIDDNYYEDIFKICKNKSIKTI
                     LDISSPKLKELLQYNPYLIKPNDEEIKDIFGIIVRDEEDIKDVLKLLHLKGAQNILLT
                     LGEKGSYFYNGKSIYYASAQPVTVVSSACAGDSALAAFLSIWLENPEHIEEALKRSAA
                     TGASVAESNGIGNLENVEDYIKNIRVRKVV"
     gene            complement(11688..12440)
                     /locus_tag="PC700_000295"
     CDS             complement(11688..12440)
                     /locus_tag="PC700_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2579623.1"
                     /translation="MIIELNKSNSENLTKTELEIIKFINENESILPELSIVEIAHETY
                     SSPATVSRAIRKCGLNGFNELRYRLTVKDENDDIQNMGDIINKSFIEAQRVIEQISLT
                     TLINIIKTINKSSKIYILARGLTEYVAKEFSLKLQLLDFNVVFINDPNIMKIKSKSIK
                     KDELIIIFSLNGNTIELVESAQNANLVGAKVITCCCSESAKLIKYSDLYIVGYKHEHI
                     SISEYEVSSRLPLYIISRIIIDYMVRYNNSEN"
     gene            complement(12737..13354)
                     /locus_tag="PC700_000296"
     CDS             complement(12737..13354)
                     /locus_tag="PC700_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2579624.1"
                     /translation="MNVIKKFIFVILIINILGCGISLILQSSLGSDAITLLNEGIHIK
                     LGISYTIAGLIYNGTLLLIALLLNKKSLGLGSCVYVLVAGIFIDLYIYLLLDFNISSL
                     GIIIRIMSFIIGHILMCSGFAMLIKFDIGMSPLDAILIYIEGKFKYPYKVLKTIADLI
                     FLILGVYLGGNLGFGTIFSILCTGTTISIVSKMISVNKKRYYSSI"
     gene            complement(13442..14899)
                     /locus_tag="PC700_000297"
     CDS             complement(13442..14899)
                     /locus_tag="PC700_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDF2579625.1"
                     /translation="MIKLSKFPKDFLWGGAVAANQLEGAWNEGGKGWCLADVHMYDKD
                     RDISKHYESDMTVDKIKFAMKDNNGYYPKRNGIDFYHTYKEDLKLLKELGLKCFRFSI
                     NWARIFPNGDDLEPNEEGLKFYDNLINEVIDNGMEPIVTLLHYETPLNLVLKYKGWSN
                     RKVVDFFVRYAELILNRYKDKVKYWIVINQINLMYHESFNSVAFCVDQVDDVEEAKYQ
                     AIHHQFMATALTKKLGKEINSDMMIGMMMADCTSYPEDCDPENVVFALKRNRIQYFFT
                     DVSFRGEYPAYIKRFFKENNINIVMEEGDDKVLKENTMDFLALSYYYSTTVSASKNTM
                     NPIDNVKNQYLKANPWGWAIDPKGLYNTLSQYYDRYQKPIMIAENGFGMHDKFEDGKV
                     HDSYRIDYLGKHIEQIGEAIADGTEILAYCMWSPIDIVSCSSQEMEKRYGFIYVDIDN
                     NGNGSKERYKKDSFYWYKNVIRTNGEELKEKVDSF"
     gene            complement(14921..15274)
                     /locus_tag="PC700_000298"
     CDS             complement(14921..15274)
                     /locus_tag="PC700_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2579626.1"
                     /translation="MTTEEHVIDEELVEVAMQIILRAGEARTEIKHALNDLERFDYKN
                     ADLKLAKAKEFMTEAHRAQTNIIQGEASGEKRAHSLLFAHAQDTLMTIFSELNITMSL
                     VGIVKSIEYKVNNSK"
     gene            complement(15303..16550)
                     /locus_tag="PC700_000299"
     CDS             complement(15303..16550)
                     /locus_tag="PC700_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2579627.1"
                     /translation="MGRLFMNKFIDLLSEKFTPVVNKITKNIWVQSVQSTIMKVLPMV
                     FVGSLVTIVSVLKNYISFLPDLSPINQYTFGLLGLFIAFLLPMEIMKNKKFESMSVVA
                     GLAGAGLMLMMIRPEITNEGAIFNFNRFGGEGMLVALVAGLFSGLIMSLFGSFSFFGE
                     DSALPDFVSKWFDSMLPIAVIMFIGWGLIYGLNFDMFEIIVRIFKPVSDIAQTLPGFV
                     LLNFLVVFLYSLGISGWVLSPITYAIQLSAIAANAAAVASGGLATNIYTYEVVQTGWI
                     TLGGWGATLPLVLMMCLSRAGRIKAIGRACIVPSIMNINEPVMFGAPVAWNPFLMLPM
                     WIGTVVISITTYLSMSMGFVTIPSKVFNLWYLPFGISTFMVNQDVRGLIVLAINIVLL
                     FMIWYPFFKAYEKEELKLESQEA"
     gene            complement(16558..16875)
                     /locus_tag="PC700_000300"
     CDS             complement(16558..16875)
                     /locus_tag="PC700_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2579628.1"
                     /translation="MKNIKVLLACGSGASTGFMATSMRKSAKKKGIEADIKARSESEI
                     DKYLPEIDCLLLGPHLDYMKDEIQKKADEYNVPVACISKTAYGRLDGVKALEEALELI
                     LNK"
     gene            17123..19036
                     /locus_tag="PC700_000301"
     CDS             17123..19036
                     /locus_tag="PC700_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2579629.1"
                     /translation="MKTSKKELFLNFIKTQHDWIDSSTLANYLNVSTRSIRKYVNEIN
                     STGEFILSSKKGYKVNLNNNCQTKVDSSENISPDNRLNLILKELIVNSNGINIFDLSE
                     ELFVSPATIEGDIVKANKFIGSYNLKIKQSKFLLKLIGNESAKRKLMSSIIFKETGSD
                     FLSLFDVQKIYQEYNLTKLKENIIYILKKYNLFINEYAINNILLHLMITIDRIKKNNY
                     IESVEVVNYIDNNNDISISRDISNFLESEYNLTLTSAELYYLVFQLTNKTTVLNYHEM
                     DTKSLPNYIDEHFVKLTKKIIKNVYDLYFIDLSDDEFVVKFTLHVKNLISRAKNNQVL
                     RNQIPQKLKDSYPLIYDISVYICNQIQILENIEIDEDEISYISLHVGSFFDRQKLLED
                     KILCALITPNYYDLQFKIVRDLEKRFNESIEIIQIFSDTHNLDFDNKVDMVITTLPIS
                     DRCPIPFVYVNPYLNRKDYDNIQSKFIQIKDRKNILTVQNHLEMYFSESLFMKNIYLD
                     SAKDYIKFMGNILYKNKYVKPNYIDDVLIREKMSSTAFNNNVAIPHSMKMDALKTGVC
                     LIVNDKPVKWGEEKVQIIAMIPINEKEKEKFNYIFESFIEILSEWNNVKELTKADNYS
                     SFMNRIAYLIQNI"
     gene            complement(19159..20313)
                     /locus_tag="PC700_000302"
     CDS             complement(19159..20313)
                     /locus_tag="PC700_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDF2579630.1"
                     /translation="MKILISIDSLKGSLSSIEAGNAIKKGILKVKEDAQVKILPLADG
                     GEGTVDALVQGMNGKKETIEVTGPIAKKVDATYGLLKNTSTAIIEIAQASGLTLVPTE
                     LRNPLYTTTYGVGEIIKEAINKGYRNFIVGIGGSATNDAGIGMLQALGFEFYDENNKL
                     VGLGGKVLNEIRHIKIENRLKELDECKFKIACDVNNPLFGKNGAAYIYGSQKGATSEI
                     IEELDNGLRNFSKVVKNYLSKDVANVEGAGAAGGLGFAFLAFLNSKLESGIKIILEEI
                     KLEEELKDADFVITGEGRLDNQTAMGKAPIGVAKLAKKYGVKVIGLAGATTEDAVKCN
                     EEGIDAYFSIVNRAMTIEEAMDKATASENMTATTTQIFNLITSIQQSKNM"
     gene            complement(20595..23210)
                     /gene="mngB"
                     /locus_tag="PC700_000303"
     CDS             complement(20595..23210)
                     /gene="mngB"
                     /locus_tag="PC700_000303"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HDF2579631.1"
                     /translation="MKKNVHIVPHMHWDREWYFSTEESRILLVNNMEEIMDMLENNHN
                     YPYYVMDGQTAILEDYLAVKPECKERIKKLVQEGRLIIGPWYTQTDEMVVGGESILRN
                     LLYGIKDCDEFGEYMKIGYLPDSFGQSAQMPQILNGFDIKYSMFWRGCSERKGTNKTE
                     FNWKSDDGSSVLVQILPLGYAIGKYLPMNEDELRTRMDKYLPVLDKGATTDHIILPNG
                     HDQMPIQKNIFEVIYKLKECYPERKFFLSRYENIFKELEKNKDIDTIKGEFLDGKYMR
                     VHRSIFSTRMDIKSANARIESKITNILEPLASIAYSLGFEYHHGLIELIWKEIMKNHA
                     HDSIGCCCSDKVHQEIMNRFFLAEEKVDQLITFYKRRIVDAMSCEMALDKLVAFNLMP
                     YNRNEIVNAQVITKMKAFEIFDKDNNKLDFEVVHKEVIDAGLIDRQIVHYGNYDSFMV
                     YTINFKDKIPAMGYKAYMIKEVEHMLEKAYEVCDMVDNDFYTIQVNENGTLKISDKKL
                     NKTFENVLLMENGGDEGDEYDFSPLPNEKLIFNDNIVANSTIKKNRFNNEIKINYRLK
                     VPKDIQARKNNRADSFIDFDIVINVPNDKAIINVVFNINNQARDCRVRTYIPTNIASK
                     FSVSDNQFGHIKRSVYDDAMEVWQQEEWSERPDSIYPMLTFVGLSDKEHGVAVLTNST
                     REFEIVGKKFDTIAITLFRSVGFLGKEEMARRPGRPSGIKLPTPDSQMIGDIKIDLAI
                     VTHEKSTLEANVANMAKEYLTKIQTYNKIPYNAMKLNQSEIEVDYSYSLLKETNKDLV
                     LSVVKKAEKEEALVVRFYNSKEETKSANVVFNKNIKTAKWTNLNEKELESISIDNNSV
                     NVECLMNQVKTILVK"
     gene            complement(23354..25279)
                     /gene="mngA"
                     /locus_tag="PC700_000304"
     CDS             complement(23354..25279)
                     /gene="mngA"
                     /locus_tag="PC700_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HDF2579632.1"
                     /translation="MEFKKITNENLVFLKQKIKTKEDAIKFLARKLYDEGKLTSYEEY
                     LEAVMYRETLSPTGFERGLAIPHGKSKSVKEAAFAAMTLETPIENWESVDSNNKVELI
                     FLIAVPESEGGNTHIQILSELVTRLSNEDYMNMLLNSKTKKELFNNLDIKEKKEQIKE
                     EIRIQNESKKIVLAITACPAGIAHTYMSAEALIKAGKEIGVDVYVEKQGANGMVDPHT
                     PDIIKKADAIIYATDVAPKNTERFEHLPNIKTSVAAPLKRAKEIIYEALEVAQKQGKG
                     DYIEKSSDISYCEKSSWKKDVKSAILTGVSHIIPLIVAGGMILAFAVLASQAFGLQEL
                     YNQEGSWLWQLRKLSGGMLGTLMVPVLSAYMSYSLAEKPGLGPGFAAGLAADLIGSGF
                     LGGMLGGLLAGYIVKFLKKNIPAKGTLAGFVSFWVYPVVGSLVVGLLMLFVIGKPVAA
                     LNNGLINWLNGMTGINAILLGAIIGAMVSFDLGGPINKAAYTFAIGAIASGNFIPYAI
                     FASVKMVSAFGVTIATVLGRDLFSESEKEIGKQTWILGLAGITEGAIPFMIEDPIHVI
                     PSLVAGSAITGSIVAFFNIGLQVPGAGIFSLALLEGSMSGFIIASIWFFAAVLGAIIS
                     AILLIITRKQKNQRKIH"
     gene            complement(25409..26134)
                     /locus_tag="PC700_000305"
     CDS             complement(25409..26134)
                     /locus_tag="PC700_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2579633.1"
                     /translation="MEFYERAKKYEYKFTDTEDMIIEYMRKNKEEIVKLSIQSLADKF
                     FTVPNTIVRLCKKMEYDGFSQLKNNLKEELKNEDKEPKNGFESDIKKTLELIDYEKIN
                     KVADIIQKSRSIIVYGIGQNIPLCEILVKEIRGGHDNITYYSQRHEVLHDIGKLGEQD
                     MFFALSLSGETKEVIEAAKTAVNKGCKVVALTHICENQLQQIADISLYCYSTIKQVNG
                     YNISDKVPIMMIMRAIIEQYWLR"
     gene            complement(26397..27176)
                     /locus_tag="PC700_000306"
     CDS             complement(26397..27176)
                     /locus_tag="PC700_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HDF2579634.1"
                     /translation="MHKMKKLVVRADDIGYSEGVTLGIISSYKNGIVTSAGIMVNMPF
                     SRQAIEMVKNDDVCFGLHVNLVIGEPCCKEHIKIKNLLDEGGKFVSSKIRRTQLLTGK
                     EVFNYEEVYEEVCAQIETYKLYMSKLPEYIDIHGIEEDVSISAMCDAAKEYGIPSCPY
                     YLDSCISMPSKYPQNDFYKLNKSFVKLFEEKYINLDKDITLMVTHPGFIDYSLIKSSS
                     LIQERVYEHAMLCDKEVKMWLKRNNIELVDFKDIFKSNNYI"
     gene            complement(27169..28596)
                     /locus_tag="PC700_000307"
     CDS             complement(27169..28596)
                     /locus_tag="PC700_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2579635.1"
                     /translation="MMQQLSLLGKTMLVPIAVMPAAGILGLLFGPNMLNVPAISNISN
                     IVFSNVDYLFLLGAVSAYSKSKDKTSVMVAAVVAYMIFKSNLELLNDTLNAGVFGGII
                     VGACIAWTYNKTYKVKVPAFLSFFAGEKCVITLSPVVALILSYIFSLLWIYPQAAMNQ
                     FGFWLGGAGAIGIFLFLFCNRALIPTGLHQVLNAYILFEMGEYTTSTGEVIRGEIPRF
                     MAGDPNAGFFWTGFYIIMMFSIPAISYAIYKTAQDKKKQEVKGAMTAGALTSFMATVT
                     EPIEFSFLFASPKLYIIHSFYSGLGGVVLYLLGARLGSFNGASIIDYVLSFSYGDKAW
                     LVIPVGVVFFVLYYVTFKYIIIKDNVQTPGHEVEIEIGDKVSEKEKNLKLSHGNFSYM
                     AKQIIKNCGGYENIVTLNNCMTRLRLEVKDATILNDDNIKKTGAKGVIKLSNTSVQII
                     IGTDVVKVKDEMEMQLDELRKQADA"
     gene            28987..29709
                     /locus_tag="PC700_000308"
     CDS             28987..29709
                     /locus_tag="PC700_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2579636.1"
                     /translation="MAKNKFSEIYEVLKEEILDGKYTSNMMLPTELQLIERFSCSRNT
                     VRRAISQLNTEGYVQSIKGKGVVVLENSCSNDFFLNMHNFKGVESIVEDKKVNTATSV
                     LHFSKILIDNKLSKKTGFKVGSEVYYLHRLRYIDNIPKILDINYFLCSIVKDLDVSIA
                     QGSIYKYIEECIGTKIVSSRKIFKIEKATELELKTLPLNDYNCVGVIKNSVYTDDGKL
                     FEYTESKHTPETFVFMDVTQRY"
     gene            29906..31573
                     /locus_tag="PC700_000309"
     CDS             29906..31573
                     /locus_tag="PC700_000309"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDF2579637.1"
                     /translation="MKNWWKKATVYQIYPKSFKDSNNDGIGDINGIIEKLDYLYSLGV
                     DLLWLTPMYVSPQRDNGYDIEDYYNIDPKYGTMSDFEKLLKEAHKRDIKIMMDMVLNH
                     TSTEHKWFKESKKSKDNPYRDYYFWKDAKPDGSVPNNWISRFSGTAWKYDETTNQYYL
                     HLFEETQADLNLENEKVREECYKVLEFWADKGIDGFRLDVVNLLSKTPGLPDDPITGP
                     KGDGRTHYADGPRIHEYLHNMNQKVFKPKNIVTVGEMSSTTPEECINYTRENREELSM
                     VFSFHHMKTDYKGGAKWTNEMFSLDKLKKAQSDFQYKMYEGKGWNALFYSNHDQPRAL
                     SRFGDDRFFRQESAKMLAITLFGLQGTTYIYQGEEIGMTNAYFTKIDEYDDAESKNAY
                     YHMIKSGIDEKEALLILQQKSRDNARTPMQWDNTIYKGFSNHKPWLKVNNDNISVENE
                     LNDSRSVFYTYQRLIKYRKQYDIFTEGTYRLLDENHKNLFVYEREYKNQNLLVISNFT
                     HDNVVYKLPETYLNKLNYTILETNYTRDIIENKMEIKPYESIMIYYK"
     gene            complement(31669..32988)
                     /locus_tag="PC700_000310"
     CDS             complement(31669..32988)
                     /locus_tag="PC700_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2579638.1"
                     /translation="MEKGIKLGFWSIVLLGINAIIGSGIFGLPGDAYTDIGPASILVL
                     VFCMLLAVSIALCFAEAGSWFDTDGGPYLYAKEAFGDFVGFEVGFMKWIVSMIAWATM
                     ANFFAVTLSSVWPQAAEPLIKNIIIGILVVGLGIINFMGMKQSKHLNNIMTIGKLLPI
                     VLFIAVGLFFIKGSNFTPFVIIQKGQSASSAFVAVSITLFYAFTGFESLAVAAKDMEN
                     PKKNVPKALVMVMFVVSVIYMLILGISIGVLGNGLAGSATPVADAAIKMLGPIGGYII
                     TIGTIVSVGGINIASSIFTPRSAAALVEQGLMPKSIRKTNKNGAPYIAIIVSVIGTLL
                     IAWSGSFTTLSQISVVSRFIQYIPTCLAVLILRKKYADKDVNFRIPGGAIIPIFAVII
                     SILLLIKAGIDEPMKIVWGLGGMIIVVPIYFYMTKVYSKKYNDVEVK"
     gene            complement(33029..33775)
                     /locus_tag="PC700_000311"
     CDS             complement(33029..33775)
                     /locus_tag="PC700_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HDF2579639.1"
                     /translation="MKKLVKKKYPIIGISGNLLIDEGGMFPGYERAYVNNDYIQSVVM
                     CKAIPYIVPIVYDDEIIKEQVSNIDALILSGGQDVNPLIWKEEPHNKLGAISPKRDSF
                     DMKLLKHALDMKKPVLGICRGEQIINVTEGGSLYQDLSLIEGAYIKHNQQHLSNVPTH
                     TVQIKEGTKLYEILGEKEVLVNSFHHLVVNKVAPGYIVSATSKDGLIEAIEKEGSEFV
                     IGIQWHPEMMTRDYDNMKKIFMAIVKEASK"
     gene            complement(34019..36028)
                     /locus_tag="PC700_000312"
     CDS             complement(34019..36028)
                     /locus_tag="PC700_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2579640.1"
                     /translation="MKTIAVVTDGLSKLECFLNKNLEMIFKDKVMIKNYYLNKITEKE
                     LIKDDIILVMIDDRVLKIKKYVEDVSKIITINRSIKQKDIYKLFSLPEEIDVLVVNDN
                     KHTVLETISNLYNIGINHLNFIPYNPNEEYRNIKIAITPGESSLVPKYIEEVIDLGHR
                     YIDSSTFIQIIAKLKLDDKEITERLIKYTDEIISLYSGINTKYKELTIKADELNSIIN
                     LSNLGMAMVSDKGNIIICNNSLRDILDIQSNIIGKNIDELENENIKKIFSLSKAHDEV
                     IKFNNKYLNVNKYNIESFGRVTGYYFCIQEITYIRKLEQNLSKKLREKGQVARYTFDD
                     IKTVSTKMERTKKLGMKIAESDYTILITGESGTGKELMAQSIHNASPRHSQSFIAINC
                     AAMPENLLESELFGYEEGAFTGALKGGKKGLFEQANNGTIFLDEIGDMPIYLQSKLLR
                     VLQENQVMRVGGENVIDIDVRVIAATNKNLLEMIKGDRFRADLYYRINVLPINIPPLR
                     ERKEDIEVMLKHFMKRKRAISQGVQDIINAYEWPGNIRELKNTAMYINIMSSEDVILI
                     NDLPHNLLNITEDFSKEISILKDRTSIEKVKLVMECLKNANNLNKNIGRNGIVNSINE
                     LGYSITEGEVRGILNMLKELDLIICESGRKGSELNRRGKNILNSI"
     gene            complement(36466..37899)
                     /locus_tag="PC700_000313"
     CDS             complement(36466..37899)
                     /locus_tag="PC700_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDF2579641.1"
                     /translation="MVLRKDFLWGGATAANQCEGAWNLDGKGPSCSDMCTGGSQKVSK
                     RITRVIEKGTFYPSHEAIDFYHRYKEDIALFAEMGFKVFRFSIAWTRIFPTGMEKEPN
                     EAGLKFYENVIDECLKYNIEPLITISHYEIPFAITEKYNGWVSREVIDLYMNYCETIF
                     KRYKGKVKYWLTFNEINSATMPMGGYLSQGILNEGTTDFINQVDIPQLRFQGLHHQFV
                     ASAKAVKLAHEIDSNYQVGCMQIFATMYPYTCNPDDAVKTQRDSRVMNYFCGDVQVRG
                     EYPTYMNRYFGENNIEIKMEKGDLEILKEGCVDFYTFSYYMSTCVSSDSKEDNTSGNI
                     LGGVKNPYLKSSEWGWQIDPEGLRYALNEIYDRYRIPMMVVENGLGAYDKKESNGVIN
                     DDYRIEYLKAHIEQMKEAVEDGVDLFGYTPWGCIDLVSASTGEMTKRYGFIYVDKYDD
                     GSGDLSRIKKKSFDWYKQVIESNGERL"
     gene            complement(37986..39902)
                     /locus_tag="PC700_000314"
     CDS             complement(37986..39902)
                     /locus_tag="PC700_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2579642.1"
                     /translation="MRRYEQLSKEIIENVGGKDNISSLSHCITRLRFKLKDESIANDD
                     VLKKMDGIVTVMKSAGQYQVVIGNHVPDVYEVVCDLAGLGGDTIDNSDSDAPKGLLNK
                     FIDIISGVFQPILGVLCAAGMIKGFNAVFTALGLYGETDGAYIMLNAIGDAMFQYMPI
                     ILGYTAAKKFKLKPFIGMLIGAVLCYSGIQLDTLASIGQPLYNLFTGTIFESPVYITF
                     LGLPVIAMNYTSTVVPVIIIVLLAAKLEKVFAKIIPDVVKNFFVPMAVLLVSLPLGFM
                     VIGPIATYASNIVGNGFLALFNFSPILCGVLTGLLWQVLVIFGLHWGLVPIAMSNLMT
                     MGFDTILVGAFVPSFAQTSVVAAMYFKLKDKKIKELCIPAVISGICGVIEPAIYGITL
                     PRKTPFIYSCVGGAVAGAVMGIMNVKSYVMGGLGIFGIPNYINPTTGDMKGVYATIIA
                     IIVAIVVGFVLTLFFWKDDSIVEISKDNNGQGVVEKNKREVVYSPIKGEVKALEQIQD
                     AAFSTGILGKGVAITPKEGKVVAPFDGTIMTLFPTKHAIGIISDNGCELLIHIGLNTI
                     QLEGKHFKSFVNQGDKIKKGQTLITFDIESISKEGYCLETPVVVTNYSDYVDIIENTQ
                     KHVNNSQELITVLA"
     gene            complement(39976..40836)
                     /locus_tag="PC700_000315"
     CDS             complement(39976..40836)
                     /locus_tag="PC700_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2579643.1"
                     /translation="MIIEKILNNNVIITTDENHKEIVVMGRGLAYKKRTGEHISKDKI
                     DKIFKLSDPNISDKFKELIADIPIRYMELSDEIILYAKEKLGKRLNDSIYISLTDHMY
                     TAIERAREGVSVKNVLLWDIKRFYKSEFKIGLEALDYIEKKFEIRLSEDEAGFIALHI
                     VNAQMDQSIKTIYEITQIIQEISNMVKYHYRIVFDEDSVYYHRFITHLKYFAERIVSN
                     NLHENNEDDLLNVIKVKYKNAYKCIEKLDEFIHKKYNYDLTDEEKLYLTIHVERVVSK
                     SIKSDINPNN"
     gene            complement(41187..42374)
                     /locus_tag="PC700_000316"
     CDS             complement(41187..42374)
                     /locus_tag="PC700_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2579644.1"
                     /translation="MIESIKKRAYEIENELGKEIEEVCNFIFNNPELGEEEYISSKYL
                     VEKMKEYGFDTVYPYCNMETAFRAELGDNDGPTIAFLAEYDALPGYGDNKEPAHACGH
                     NWIAASTLGACIVLSKLKENFKGKIVLIGTPAEETTGGKCDLVKAGAFDDVDVSYQMH
                     IEAFNNINCKALAIDSLEFSFEGVSAHAASHPHMGVNALDAVQLTFAGINALRQHVKS
                     DVRIHGIVSNGGEAPNIVPEKAACKFFVRAAERSYLDEVTKKVINCAKGAELMTGAKL
                     SYRYFENSFDNIINNKVLQKITKNNLIESGITDILEGKDGPVGSTDIGNVSQVCPTMY
                     TEIALDISPMVYVHEKEFLNYANSEEAYDKLHKAVKAMVGCALEIYLEDGLLDEIKKN
                     HLN"
     gene            complement(42388..43779)
                     /locus_tag="PC700_000317"
     CDS             complement(42388..43779)
                     /locus_tag="PC700_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HDF2579645.1"
                     /translation="MSSLKKKKFTMPQTLTIIFSLIVLMAILTWIVPSGNFERVDIDG
                     RSVVVAGTYEKAPSNPQGITDVFTAPINGFIDAAEVVGFVLIVGGAFGIVNKTGAIEA
                     VIAHTVNKMKKFQFLIIPISMILFGLGGTTFGMSEETLPFYMIFIPLMTSMGYDSLTA
                     VATVFIGATAGFGAATTNPFSVGIAQALSQIVPGSGIEFRVVMFIIYMAISIGFVMMY
                     ANKVKKDPKKSLVHDISLNQESMVNSDTNIKEFTKREAMVIAIFTIGMAIMIYGVLRL
                     EWYITEIAMIFTAIGIISGIASGLKQDEIVNSFISGAGDLITAGLCIAFARGIVIIAE
                     NGFIIDTILNSAANLLNGLPKTIFINLTFLIEGLIAFLIPSASGLASLTIPVLAPLGD
                     LVDVSRQMIVTAYQFGIGVTNLITPTSGVLMGALAVANIPWSKWVRFVIPLMVVLTIL
                     VMVFLTIGLYLGF"
     gene            complement(44374..46074)
                     /locus_tag="PC700_000318"
     CDS             complement(44374..46074)
                     /locus_tag="PC700_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2579646.1"
                     /translation="MHYIAWHFNKNGGNNTMKDKLQVLSGAMMIPVILLVVAGVFIGL
                     GSAFANMENVSALGLSGLIKEGSFINVFFKVINDLGFMVMRYLPIFFVTGIAFGLSKK
                     EKGWGALGGIVLFIGMHTVISTLLASNGINSDTVTAEAFMASGLGEAEAYSRSSLYGS
                     FLGIFSYDCSIFGAIIAGFVASAVHNKFVDIELPSALSFFSGPRFSMIMMFIASIVLG
                     TAMYFIWPPVGLALSKLGNWIGSSGLLGTFTFGAMDKALLPFGIHHLIAFPIEYTRVG
                     GTMEVGGAIYEGVNNIRLAQMGDPDTLSYITRNFTTGRLLIHFAILPAAALAMYKLAD
                     NANKKKAISILIPAIVTAMLVGVTEPIEYTFLFVAPLLYFVAYVPLAGLTYVFTEMFN
                     VSIMGESFRNMFPNLLQPQKVDALPLLFLIPIFFIVTYIIFTWAIKKFDIKTPGRSSE
                     EVKLYSKKEYRERQSVDAGKTAYNNAVEEEVDDKDTKLVHSIIEGLGGSNNIKNVTNC
                     ATRLRVELNSIDGFYEDGFWVNELGASGVVKKKNSVQVIFGPRVITIASKLKAVLGVD
                     "
     gene            complement(46046..47449)
                     /locus_tag="PC700_000319"
     CDS             complement(46046..47449)
                     /locus_tag="PC700_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2579647.1"
                     /translation="MIVNKELIIDYLKSNQENIIKDIRSLVEIPSIRDESTTDINQPF
                     GIEIRNAFDKLIQIAKDKDFVVKDFDGYAIHIEYGEGEEVVGVLNHIDVVPIYNKELW
                     KSKPFKVCQKDNYLYGRGVNDNKGPLIGILYALLFLRELNEKPKRKIRLIVGGAEETT
                     WECMEHYFSVNEQPKFAFSPDGNFPIVNGENGILYFNLRKKIDKDKFRNHNLVDIKSN
                     KEDGFVCDKIEAVFKTDDKKDLVESLAYYTEIEELEEGKVLVRYTGERALSRNPHRSY
                     NCAFSLAKDLEKIKKLNDKGVIIRDILNSYFTDDNHGKKLGIYKEDVDMGVSTICIMS
                     IFLEKNELNMKIDFRYPKGISWEFITNRINEIGKKENLIVDIYKDLKLLYVKPDSELI
                     NKLSSAYKQGFGKEAELFTKGAASYARVLKNGVAFGPTIEGDNPNSHQANENISIDTL
                     YKAIEVYIYALYSLAFQ"
     gene            complement(47713..48204)
                     /locus_tag="PC700_000320"
     CDS             complement(47713..48204)
                     /locus_tag="PC700_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2579648.1"
                     /translation="MFNIFKKKENKIEKIYSPFKGNLVNLEDVPDAVFAQKMMGDGVA
                     IIPNEGKLYSPVDGEIVNVFDTKHAIMIKSNDNTNILIHVGLETMSLEGKPFDVKVKA
                     GDKVKAKDLIMEVDLDFITSNDLNIITPVVIMDDADSVKRTVKDRIEPGNVDINSIIM
                     EVE"
     gene            complement(48230..49042)
                     /locus_tag="PC700_000321"
     CDS             complement(48230..49042)
                     /locus_tag="PC700_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2579649.1"
                     /translation="MRYKLICTDMDGTLMGKGFEVSEENIKALKEAMEKGIKVALVTG
                     RPYNAMKYFTSVLGDDIYIISTNGTYFKLMGYEYKKVLSKEAIKKIYTIGEKYNLNKH
                     FKGCKIVISNNEIGEEHPYRLINSKNKEEDRIEIIENASCETLLEKADNEILKCILFS
                     ENVDSLREAKEEFKKQEDLEVVSSGKINFEVMSKGTSKGIAVKKFCDILGIDSKEVIC
                     IGDNENDISMIKFAGLGIAMGNATDEVKSMADFVTDTNVNDGVAKALRKILS"
     gene            complement(49055..50206)
                     /locus_tag="PC700_000322"
     CDS             complement(49055..50206)
                     /locus_tag="PC700_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDF2579650.1"
                     /translation="MINFYQPSRINFGQGTLSELPRIIGKYGKKCLLVTTPNVEPLDK
                     LYKRVTLNLIEKGIEVVHFDRVEPNPTVELINEGFELAKKESVDVVLAVGGGSSIDTA
                     KVLALTFGLEKIDWDYMFSTYTNPFKIYDKVSEKELPLIAVSTTSGTGSQVTQAAVIS
                     RGSEKNTIFHQNCFSKECIVDPELMLTLPERITASTAFDAFTHAFESYINPNANDFTE
                     IMSEKSMELVINYLPKVMEDKNNIEYRTKLAVADTLGGSSLANAGAAAPHPLSEIIGG
                     VTHIPHGEALAIVFPKFAEKFYKDNIEKFAKVARMLNQELEKVEDDEAAKTLGNEIES
                     FLKRVGLKTKMSEFNVTKEQLEQITSCPVLGFLPFGKKEDLVEILEKSY"
     gene            50476..51117
                     /locus_tag="PC700_000323"
     CDS             50476..51117
                     /locus_tag="PC700_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HDF2579651.1"
                     /translation="MKYLIDSANLDEIQKCLKYGFKGITANPSMYLKEKVNFHEFIET
                     CTNLNPEILTAEVIGNNLYELINSCDKILNINKNTIIKINFSEVGLELINILNSKGVK
                     TACTLIFNVNQASLAINAGADYLFPFIGRNDENGNDGIKNLTEICNLISINGYNTKVV
                     AASIKNVFHLTQASLCGCDYAAVTYDLFKKASFNQLTVDGANTFERDWSKVNK"
     gene            51143..52687
                     /locus_tag="PC700_000324"
     CDS             51143..52687
                     /locus_tag="PC700_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2579652.1"
                     /translation="MNKRLLDIAELLLNSSDYITVDTIAEELKVSNKTIRNDLVILDE
                     WLLEFNLSLDKKTGSGVIILGNEDIKLKVIRDINDKSNCIYAYSPEDRKRYILSKLFI
                     KNSKFRIRDICNELHVSRATVHKDLVVIQNFLQNFKVTLVRKTNNGVYLEGKEKDIRK
                     AVFELATVNKSYTELKEILFSNSNECKDTPSTKIFKELFNYDFEKLSQITLHTLQVEK
                     SNLSDEYYINFLIHIAICIKRISIKRYINLSEGFFRELLEHEYFEKSKILCANLSEAF
                     DVEFIDEEVCYILLHIEGLLKSIKKTTTLEPLKNEEITEYNLSEAIAVHWGSILNLNL
                     KDDLILIKSLANHLKSVLHRINYGFTITNPILNDIKRTFPYTYKAAKESNTVIKQLMN
                     YEVNDDEIGYLALYLISAIDRSKSPLNTILICHCSDGVSNLLVQKLSFEFNQINIVKS
                     IPLSSISFTNFDDVDLILTTAPVDFEHNAELININALLSKNDISRLSTIIKKLYSEKN
                     KILSYK"
     gene            complement(52904..53530)
                     /locus_tag="PC700_000325"
     CDS             complement(52904..53530)
                     /locus_tag="PC700_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2579653.1"
                     /translation="MKKEMIKDYLLITISAFLMALAVNFFFAEHTLAPGGITGLSVVL
                     STFLSMPVENISLGISMPLLVMGLLFLGKGFGIKTLYITLMGPLFLKVIPQTHITDNL
                     LVAGVIGGLLVGTAIGIAIIRGCSTGGTDLVAMLINKVIKFLKLPVILFMLDGFIIVA
                     SGIISKNFMISIYSLISLLLIIKTIGFVTTKFGKNTEEINNINNACAQ"
     gene            complement(53665..54381)
                     /locus_tag="PC700_000326"
     CDS             complement(53665..54381)
                     /locus_tag="PC700_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentose-5-phosphate 3-epimerase"
                     /protein_id="HDF2579654.1"
                     /translation="MKHIKIAAGLAHVNYGHISAVVKEAMDAGVDYVHSDAADMHDLK
                     NMQLMGGHQIIEAIRPVTDKPIECHIYTRDCDRLFIEKIAAAGCNLLIIPAEHFLGAP
                     LAYIINYCREFGMKVGLTIGCYTPLCFVDEAIYDIDRLQIVVHGVDETDGKDNWGWRK
                     SAVDLVKRARKMIDEKNPKCELAIDGGLRADNMEPLIECNPDVVILSSAIFKDKDGIT
                     AGVKNCRKAIDEAATKFGLE"
     gene            54796..56010
                     /locus_tag="PC700_000327"
     CDS             54796..56010
                     /locus_tag="PC700_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDF2579655.1"
                     /translation="MRNCIIAQSGGPTAVINASAVGIYEKNLETKYFDKVYFGINGIE
                     GILNKNIIDSDSLNKDNVLNLKQTPSSGLGSCRYKLADYKINSYDYDRVINILDEYEI
                     DTLFYIGGNDSMDTVMKFSQYCKNTTCKIKFIGIPKTIDNDLLGTDNCPGFGSAAKLI
                     STLIMETYLDFSIYKNNGIFIVETMGRDTGWLAASSSLARLNDTPLVDLIYLPEIDFY
                     EDKFIEDVRKIFKEKNKAYIVASEGIRDKYGNFICEQKTSAHDKFGHSQLGGVSNYLK
                     NLIVSSSITTRVKTLELGVIQRCAMHLASDTDLKQAAEVGAYGIELSKKGYTGVMAII
                     NRTCNNPYKYEVSHTDISKIANKTKYFPKEWINEECNYITDEALNYLYPLIQGKPDIK
                     YKNGLPYFSIIY"
     gene            56629..>56856
                     /locus_tag="PC700_000328"
     CDS             56629..>56856
                     /locus_tag="PC700_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2579656.1"
                     /translation="MMKIAVITTEFLKEFVDNSIKKLNINAEIEIYIYRDFSHVGDLY
                     LEIEDRFDGFAVSGPIPKKAITKKAGTIKKPL"
BASE COUNT        18526 a        10186 c         6219 g        21925 t
ORIGIN      
        1 attgcataat tattcaatac ctctctttgt atatttacat tatccatatc tagttatcta
       61 tttcaaccca atattagact acctactaat aaatttattc tctacataat taacaataat
      121 tttgcactaa ataacatcat tagcctatct ctgatgcatt ttctttaatt ttatttatag
      181 ttttaatcaa tttatttctc tcttcaatat gattttcaaa taaatcaatt atacttccaa
      241 cagttctaaa tgggtcttcc atcaacactt tgttatcctc tataaatcca ttcttgacta
      301 cataatcgac tataagttta acaaaatgta tttgcttagt attaaaactc ttattattta
      361 aaaactctga aaaagcttca tttgcagctt ctctatccaa tcccactagt tttctcacta
      421 attgacttac tggagtatct ccaaactcct ttttataatc atcacaatta cctaactgct
      481 ttaagagtat ttcctccaac gtttcaacat cttgctttgt tatcttttta ttatttctta
      541 atttaaatat tgctaattca tctttatggc tatttaaata atactctacc ttctttttat
      601 aattcttaag attgtttgca ttatatacag aatcatttct atcttctgct acaatcatat
      661 cttcaaaact tgtataatat atcttttgac tagacttttc aagatactta ataagctctc
      721 ttaatgatat acgtacttct tctacctcaa atatatctga atcttcccaa aaatcctctg
      781 tcattgcttt ttcaatagta tatttttgtt gctggatctg tggtatagtc ccaagtttcg
      841 aaagtttttc tgctgtaacc attacatgcc ttatcccttt acttgcatta ttattttgta
      901 agtatgcaag ttgtatatta tacataacta catcaaatct tttagccaat tcatcatcac
      961 tatatgaagt tatgagtggt gatatatatt ctcttatctc attttgtgct actgctccaa
     1021 tattgctcca aacattttca tttttatact tatgtacata acttaaattc atcctaacta
     1081 tataactatc ctcatttaaa gcttttacag aagttataag ttcttctaac aattcttttc
     1141 taagttctgc atacttacct tctatatatt taatatcttg aagttcttta accatactta
     1201 cctttatatt aaataatttt tcagtaagtg tttctgcttt atttccctca aatcctttag
     1261 gattaaattt aaaaaattca aaattattac aaaaatcaaa tattaagaat ctttccttat
     1321 ccatatcaac acctaataaa tctggacata atctagttcc tctacctatc atttgccaaa
     1381 acttagtctt acttctcact ttcttgaaaa aaacaagatt taggatttca ggtatgtcaa
     1441 ttcctgtatc aagcatgtct actgaaactg ctatttgagg taatttattc ttatcagaaa
     1501 aatcatctat tatagaatct acataattta tactatagtc aactgctttt acaaagttcc
     1561 caccatactt tggatacagt ttattaaatc tttcaactat tgccctagca tgctttgtat
     1621 tctttgcaaa aattatagtc ttaccaatct tttcttctcc ttctattttc aacccctctg
     1681 tcattagctt atttaaaact aaatctattg tgtcagaatt aaatagccac tcatttactg
     1741 cattattccc tatttcgtct ccaatactct catcatcatt aaatgtattt tcatattctt
     1801 ccttctcttc atcagaaagc tcatcatact taattccatc ttccataatc ttagtcttaa
     1861 actcaatact agtataatct actaaatatc catcctcgac tgctttatta tactcataag
     1921 catatgtagg tactccattt tccatgtcaa aaactgaata agtattttta tctatctcat
     1981 cctttggtgt agcagtaaga cccattaagt aagaatcaaa ataatcaaag attgacttat
     2041 actttttata aatacttcta tgcgattcat ctattataat taaatcaaaa tgaccaggtg
     2101 taaacaatct ctttccatcc ttagacttcg tatcatcaat tgcattcatc attgttggat
     2161 atgtagaaaa tatcatccta gcatcttctg ggctgtcttt gctatccaat aaattacata
     2221 acgccaagtt aggtaatagc ttagtaaaac tttttttagc ttgctttacc aatgcttttc
     2281 tatctgctag aaataatata tttttaatcc aattatgtct tgaaagtaca tcaactatag
     2341 atatagcaat tcttgttttt cctgtaccag tagcacatac caaaagagtt ttgcgttgct
     2401 tattttcaag agcctcacat acagccctaa tagcttcttt ttgataatat ctattggata
     2461 tttcatcatt tattgttaca gaacttaaag acttcttact ctttcttcta tctatcataa
     2521 gttgtaattc tgatttttta taaaatccat aaacttttct atctgaatag tcatcccaaa
     2581 tatacatatc aaaaccattc gtcaaaaata ttactggtct ttgactatat ctcttttcca
     2641 aacaatctgc atataattta gcttgttgtt gaccaatttt cgggtctttg ctcgttcttt
     2701 tcgcctcaac tactgctaat ggcttaccat tttcaccata aagaacataa tctacatatc
     2761 ctacaccact ttcattaggc ataccttgta cttctatttc ctcccctata tcttttccaa
     2821 attcccaacc tgcaagcttt aattctatat ttatatatct ttttcttgtc tcaaattcgt
     2881 ttacttcgtc tatcttgaaa tcgtagttta cagtattaac ttttctcttt tcagttagtg
     2941 actttcttaa tttctcattt tcttttatta cttcttcaag ctttttatct tttgaactta
     3001 atctctcata aagattttta agctcgtctg gtctcgtcct tttctcttcc ccatgaagta
     3061 ataaatcttc attaaagtct tctgctgtat attcatctga ataacaatag tctatccaac
     3121 taacaaattg atgcaaattg ttaagtgaaa gtattgcttc tcctctctct atgttcgcac
     3181 tagtatgtac agataaatta cctaatttaa ctatatattt taataaaggt aacatatcat
     3241 agtctataag ttctataaaa ctattattat gaatcaacgt actaagatta tcttgataag
     3301 gcaatactaa atcactatca ttagcatata gccattttac tgcaagctct aaagctcttc
     3361 ttgttatagt tgcacaagta gatgggctaa ctagtatact tttttctgct tctaagcaag
     3421 cagtattaaa actattgaaa tttttcttct tttgtaaaaa gtcaaaatta cccaaggctt
     3481 ttctccccct tttgtccctt agttaaaata aatctctcta aaaactattt cttacagctg
     3541 tttcttatac taacaaatac ttcttcattt tctgtaaaca attcgtacat atcttcaaac
     3601 tgtggtatat catatccatg agaatcatca tttctcaatt ttctaagtgt ttctataact
     3661 atagaagaat ttttaggtaa taaattgtga tactcacaag ctaaaaggta gtcataaaag
     3721 tttttagatt ttatgtttaa atcaattgat ttataataca acattgcttt tgttaattct
     3781 aaaaatgaga ccatatagct tttaaaacct tcttttatgt atctagccca taactcaggc
     3841 atttcttcct tatcatataa gattttgaag ttttgcaggt catataagca agaaccaaaa
     3901 tccctattta cttcttctat tgagttccaa gataatttgt ttctaattac catacatgtc
     3961 cctccaatct aagatatcta ttacatcact gttttcaaaa tagaatttat ttatataatc
     4021 cttaaaatct ttagattcat aaataacttc accttctaaa atagcttttt taagtgctat
     4081 tgtataatta tctgtaacaa tttttaggtc tacatcaatg tctaatatat ttcttagagc
     4141 ttctgtctta gttgcaagtt ctgtaattgg tacttttttg ataggaatcc aagctatgtc
     4201 tatatcgctt tctgatgtat gtttatctgt tccataacta ccaaatatta tgattgctag
     4261 agtattttct tttggaaata catttattat tgagttttta atctcatttg gtatatttct
     4321 catttaatca cctcttgttt attaggtaat attgcttaat tgaatttata tccataatct
     4381 tttaatctat ttatacatat ttctctttct tcatcagtaa acaactcttt atacttttct
     4441 tttaatacta atacttctaa tgataaatct aatctcttac actcccatag ccttgtaaat
     4501 ccttcagtag ctccttcttt attgattaat tctttagcag ctaataaggc tcctttgtta
     4561 tttatcagtt gtaaaaatct tgttggctta tagttacatt gtaaatcagc ttctttatag
     4621 atatttacca tgtcatgata taaatctagc tctatttgat tcatagattt tattccccca
     4681 ttttaatact tgtatttcag ttgaacaatt ctcctttaaa agctttttgc attaatgagt
     4741 taaagttatc ttctagttct tttaagctag tttccatttc aaacttcaaa gagtttactt
     4801 gtttaacgaa gttggcaaat tgattttgaa gtctaatagg aggtacaata atatttattt
     4861 ttctaacagc atctttagtt atcgtttttg ttacagctcc tcttactttc tcttttattt
     4921 tactttccat ataggatgtt agtaaaaggt tatataagaa tatatgattc actttatctt
     4981 cattaaatct aagcataaat tgattcatag ctaaagatac tggtttattt aaaaatggca
     5041 ttaaataaac tttacctgca ctacctatct tatttattat aacctcacca ccaaacacct
     5101 ttgatttttc aagataatta tatgcatgtt catcgatata cttaacacct ttttcaaagt
     5161 tattattttc taaatctgta gttcttacca ttaaggcata accttttgta tctaaaagag
     5221 ttacattatc tcttaaggtt tcataactac catttgaatg ataatcagtt aaaacatcta
     5281 aatatttccc tatttctgaa atttcccagt ttttttcgtt ctttgatggg gtaccaaaca
     5341 tctcaataaa tcttgattta actaattcat ccaatacttc tatttgctct tttcttttat
     5401 ctattaactc ttgagccttg tctaaaacac gcactacctt ttcttgcact tttaaattat
     5461 acatgcgtat ttttatatta gctaatcctt cattatttaa agctgattgt gtagcacctt
     5521 tacttaacct atttttttga tttagaaaat aactactatt taagtaatgg tataaatatt
     5581 gaggcaatat ttcttttgat ggttttaaaa cataaaagcc agtcgatact attatatttt
     5641 tattagtttt attaataact agtgttttct ttgtgtcctt catttttgca aatataactt
     5701 ctcctatatt tacagtttga tttgctctag aaggtttatt ttcataagta actatttcta
     5761 gattgcttat cttgttaaaa tctaagtttc cagtactaag atatgagcgt tctccatcaa
     5821 acttttttac attttccctg cttatactaa caacatctaa aatttttgct tcatcaacca
     5881 tactctacac ctctaacatt ctttctagtt cctctaatcc atccactatc tctttctcta
     5941 acaccttaac cctacctata ataacactag gagcatcata aactacttcc tcatacacta
     6001 cctctttata cttattaata gataaatcat acccattctc tcttatctct tccacatcaa
     6061 caaaaaagct ttgctctgtt ctttttctat ctacttcccc atcaagatta tcaaatctct
     6121 taataatatc ctctatatca ctatccttta tctcatttct cttatcatcc aaagaataac
     6181 catcagcttt catatcatag aaccatacct tatcagttcc acctgtccct gttttagtaa
     6241 atatcattat agctgtagat acccctgcat atggcttaaa tacaccactt ggcattgata
     6301 ttatagcttc caatttatga ttatcaacta tttcttttct tatcgattta tgtccacctg
     6361 ttgaaccaaa caatactcca tctggcacta tacatgcgca tcttccacca atttttaaaa
     6421 ctcttaaaaa aagtgccaag aataataact ctgttttctt tgtcttagtc acttttaata
     6481 aatctgctga cactgcttca taatctaaac ttcccttaaa aggaggattt gctagcacca
     6541 atgtaaactt atctttatct gtattaacct ccgataaaga atccttatac tctatattag
     6601 gattgtcaac gccatgcaac atcatattca tagcacctat tctaagcata gttctatcca
     6661 tatcaaatcc atagaacata ccattattaa aatgctcctt cagcccttgc actaagaaaa
     6721 gacttgaatg attatccctt aagtactgtt gtgatgatac taaaaaacct gccgaacctg
     6781 ctgccgggtc tactataata tcttctggtg ttggtttaat aagtcttact atcatatcta
     6841 ttatgtgtct tggagtccta aattgaccat tagtacctgc tgtagccact ttggataata
     6901 aatactcata caaatctccc tttgtatctc tatccttact taattctaaa ttactaatac
     6961 catcaactat tttagataac ataagaggcg taggaatttt aaatattgca tctcccatgt
     7021 actttgcata agctgattct ttatttccat gtaatttttt tatgaaagga aacacttcat
     7081 tttgtactat ttcatacatt ttttctgagt ttccttcatt agaaaactta ctccatctta
     7141 aatgttgatg ttcatcatca aatatctttt caaaagaaac acccaaaaga gttgcttctg
     7201 cttcattttt tgtttctact tcatccaatc ctttaataaa taaaagatat gtaaattgtt
     7261 ctataacttc taatggatta gttaatccac cagtccaaaa tgtctcccaa atcttgtcta
     7321 ctttactcct taattctcct gtaatcatat atgactttcc ctccagtgtt cataaaataa
     7381 tttaaatata tattttagtc gtacgcctta atagttagtg tcttatgtta aatccaagtt
     7441 tttactacac aattattaaa ttatttttcc catttacata ttttagctaa aaaactaatg
     7501 caatactctt attttatgat tattatatat accatacaaa attattttat catatatcca
     7561 tcatagaagt catctacctg gataactctt accatattat aactttttaa tattaattta
     7621 aacttagaaa gaaatttttt atttaattat atcatcttcc ttatttcaaa tttctcaact
     7681 acaatcctaa tcaaaaaata aaataagatg ctgtctcaaa atagatataa tcttcttttt
     7741 tattgtcaga atgatgaaaa tatatttgat ttcacacaaa aaagagtgcc tcatgaacta
     7801 aaaagttcat tttgagacac cccttaattt aatcttactt attctttccc tttattatta
     7861 acacttcact gtccctattg cttttcctac acatccacaa acttccatcc tactcttaac
     7921 aaaatcttta actgactcta aacctttttt accatatttc ttagggtcaa ttacctctgg
     7981 gtcagaatta agcacatcct taaccccatt gctataagca atccttaatt cagttgcata
     8041 attaactttg caaatacctc tttttataga ttccttaata acctcatctg gtattccaga
     8101 cccaccatgt aatactaatg gaactgatac aacctctcta atttcagata atctatctaa
     8161 atcaagcttt ggctctccct tatatagtcc atgtgccgta cctattgcta ctgccaaaga
     8221 tgatacccct gttctctcta caaactcttt tgcttctaat gggtctgtat atgctccacc
     8281 atcaccacca tctaaatcgt cttccttacc tccaactttg cctaattcag cctcaacagg
     8341 tatatctgaa ggcatacatg catctacaac agatttagat attgaaatat tatcttcgta
     8401 actttcatga gaaccatcaa tcataataga agtatatcca actctaagtg cctgcatagc
     8461 tagtccaaag cttgaaccat ggtctaagtg cattgccact ggaacacttg ccttctctga
     8521 tgctaccttt acattcgcta aataataatc tagccctgca tactttattg ttgaaggtgt
     8581 tgtttgcaat attactggtg atttaagttc ttcagcagcc tctattactg ccataaccat
     8641 ctccatattt tcaacattaa atgcacctac tgcataatgc ccttcttgtg catctaataa
     8701 tatttgttta gttgtcacta atgccataat ttctcctcct atatgtttat actgatttga
     8761 tttcttcgtt atttaatttt gatactattt ctctacttgt tttgctattt aataactcat
     8821 ttaaaaattc ttcattcatt aattttgaag atagttgaga taatgttttt aagtgtaaat
     8881 tatcaccatt atctgtagct gatatcataa atataatatg tgccaagctt ccatcttctg
     8941 attcaaagtc aaacccttct tttgatacac ctactgctac acgtggtacc tttactgcat
     9001 ctgtttttgc atgtggtatc gctatcccca taccaaaacc tgtagatgat atttcttctc
     9061 ttttaaggat ttcttcttta aacttctttt tatctaaaag aacaccagat ttatcaagta
     9121 ttccaatcat ttcatcaatt acatctgttt tattttttcc cttaacatct aaaattactg
     9181 tttcttcgct atataaagag atattattag atacgttttc cgattctgca ctttcatttt
     9241 tatgtccatc ttttgttgca tttgtatttt ctatagtatt tttctcattt actactttag
     9301 gtgtaaccat catataaagt acagcaccta ctaatgaacc tacaaatata caccctaccc
     9361 ataaaagtgg cttattgact aatcctagaa ccaaaaaccc tccatgtgga gctggtactt
     9421 ctattttcat tatataagtt aaaattgatg atattgatga tgcaatcata agtactggca
     9481 acacttttaa tggatttttt gctgcaaaag gtattgcccc ctctgttata tgagtactac
     9541 ctagtatata attgattaat cctgctgctc tatcttcttc tgtaaatcta tttttaaata
     9601 aagtagatgc tattgctatt accaatggtg gtgttataca agctgcagat acacctgcca
     9661 taaataaata atttccctgt cctagaagca tagttccagt tacatatgct gctttattta
     9721 ctggacctcc catatcaaat gctgacatac atcctattac tattcccaaa attataggac
     9781 ttgaattttg taaccccgat agaaagttca tcatagcatt gttaagtgct gaacatggtc
     9841 caccaagtaa caacattgct acacccacaa gacctacgct gacaatagga attataaaaa
     9901 tcgatttaag accttctaat tgtcgtggta attttcttaa caaatgtaca aatagtttaa
     9961 ctaagtaacc tgctaaaaat cctgatatga ttccacctaa aaaccccgaa cctgtatcaa
    10021 atgatatcat tcctccaaca agtcctgcca acataccagc tcttcccgat atagattgag
    10081 ctataaatgc tgccaataca ggaaccatca agttcataga atatccacca acagatttta
    10141 atgtagctgc tatttgatta tattgttctg aatttgggtc aaatgaataa atcccccata
    10201 aaaatgatat agcagtaagt actccacctg ctactactaa aggtaacatg tgagatacac
    10261 cattcattaa gtctttatac aacttttgac caaacgataa atttccttct tctgcaattt
    10321 tactgcttga taagtttcct tttctaatag ctacttttcc atcaatacac ctttgtataa
    10381 gttcattagc cttatgtata ccttctttga caggtacttc tattaccggc tttccattga
    10441 atctgtccat atcaacattt ttatcacaag caactattat tgcatctgcc atttcgatat
    10501 cttttgctgt taacttattt tctacaccta tagctccatt cgtctcaact tttatgtcac
    10561 accctaattc ccttgctgaa ttttttaatg cttcttctgc cataaaagta tgagctattc
    10621 cagttggaca acctgttacc cctagaatca ttttctttcc catctttata ccacctttct
    10681 gactcttata ttttttatat aatcttccac attctccaaa ttacctatac cattactttc
    10741 agccacactt gctcctgttg ctgctgacct ttttaatgct tcttcaatat gttctggatt
    10801 ttccaaccat atacttaaaa aagcagctaa tgcagaatca ccagcacatg cagagctgac
    10861 tactgtaact ggttgagcac tagcataata aattgatttt ccattataaa aataagaacc
    10921 tttttctcct aaagttagca atatattttg agcacctttt aaatgcagta atttaagtac
    10981 atccttaata tcttcttcat ctcttacaat aattccaaat atatctttga tttcttcatc
    11041 atttggtttt atcaaatatg gattatactg tagtaactcc tttaattttg gtgaacttat
    11101 atcgagtatt gtctttatac ttttattttt acaaatttta aatatatcct catagtaatt
    11161 atcatcaata ccaggaggta gacttccact tattgataag taacttatat ctgctaatga
    11221 ctgtatctta tctagcatat ctagtttttg tttttcgttt acatatgacc cagagtttac
    11281 aaatttaaat tcatcacttc catcatttaa aaatatattt atacgagttg tatcttctac
    11341 ccaagtaggt aatacgtcaa atcctctctt ttcgctttct tcaactatat attttcctga
    11401 aaatcctcca aaaaatccta atatcttaga ttctatacca taatgaccta gtacaaaagt
    11461 tacatttaat ccctttccat taggagaata tacagcattt gatgtacgat ttactaattt
    11521 tctctttatc ccattagtag atatattcat atctattgca ggattcgtag ttaatgtata
    11581 tatcattgtc ttcccccttg cttgtgctgt gtatgttttc attataataa aaaagtctat
    11641 gaaaatcttt tcacaaatag aaaatcgatt ttcatagact tttcaattta attttcagaa
    11701 ttattatatc tgaccatata atctataatt atcctagata taatatacaa gggcaatctg
    11761 gaagatactt catattcaga aattgatata tgttcatgtt tgtatcctac aatatataaa
    11821 tccgaatact ttataagttt tgcactttca ctacaacagc aagttataac cttagctcca
    11881 actaaattgg cattctgtgc tgattctaca agctctatgg tatttccatt caatgaaaat
    11941 atgattataa gttcgtcttt ttttattgat ttagatttta ttttcattat gttggggtca
    12001 tttataaaaa caacattaaa atcaagtaat tgcaatttaa gactaaactc ttttgctaca
    12061 tattcagtaa gccctcttgc caaaatatat atcttactag atttatttat agtttttatt
    12121 atattgatta gtgtagttaa agatatttgt tctataactc tctgtgcttc tataaaggat
    12181 ttatttatta tatcccccat attttgtata tcatcatttt catcttttac tgttaatcta
    12241 tacctaagtt cattgaatcc atttaaacca cattttctaa ttgctcttga aacggttgct
    12301 ggagatgaat atgtttcatg agcaatctct actatagata attctggtaa tattgattca
    12361 ttttcattta taaatttaat tatttcaagt tccgtcttcg ttagattttc agaattactt
    12421 ttgtttagct caattatcat atgttatctc cccttatata agttttattt gtctaaattt
    12481 atcgagtaat ataatatatt acctcttaac tacttatttt tatatacaat taagatttag
    12541 tcaatgtcta tatatgtaaa tttataatat aataatttta tgcttcaatt aattatgtaa
    12601 ttattagagt attttgtaat aaaaattgga gttactcaat tactgaaact gagtaactcc
    12661 aattcttaca gcctcaaatt aactcgaatt attgaaaatg attcataaac attatcctat
    12721 actctcactt atcaatttat atacttgagt aatacctttt tttattaaca gaaatcatct
    12781 tactaacaat tgaaattgta gttccagtac ataatattga aaatatagtt ccaaacccta
    12841 aatttccgcc taaatataca cctaaaatta aaaatattaa atctgctata gtttttaaaa
    12901 ctttatatgg atacttaaat ttgccctcaa tatagattaa tatagcatct aaagggctca
    12961 ttccaatgtc aaattttata agcattgcaa atccactaca cataagtata tgtccaatta
    13021 taaagctcat tattctaatg ataatcccta aactacttat attaaaatct aataataaat
    13081 atatatacaa atctataaat attcctgcta ctaatacata aacacagctt cctaatccca
    13141 aacttttttt attcaataac aatgctataa gtaatagagt tccattatat attagccctg
    13201 ctattgtata acttatacct aactttatat gtataccctc atttaaaagt gtaattgcat
    13261 cactaccaag gcttgactga agtataagag atataccaca tcccagtata tttataatta
    13321 aaattacaaa tataaatttt tttatgacat tcataattaa taaaccacct tatttataat
    13381 tataatccct cttataaaac tgtattaata acatagtatt tataagaggg attatttata
    13441 tttaaaaact atctactttt tcttttaatt cttctccatt agttcttatt acatttttat
    13501 accaataaaa actatctttt ttgtaacgtt ctttgcttcc atttccattg ttatctatat
    13561 ctacatatat aaatccatat cttttttcca tttcttgaga tgaacaactt actatatcta
    13621 ttggcgacca catacaataa gctaatattt cggttccatc agctatagct tcacctattt
    13681 gttctatatg ttttcctaaa taatctattc tataactatc atgaactttt ccatcttcaa
    13741 atttatcatg cattccaaat ccattttcag ctatcattat tggcttttga tatctatcat
    13801 aatattgaga taaagtattg tataatcctt tagggtcaat tgcccacccc caaggatttg
    13861 cttttaagta ttgatttttt acattatcaa ttggattcat ggtattttta cttgctgata
    13921 ctgttgtaga gtaatagtat gatagagcta gaaaatccat agtattctct tttaacactt
    13981 tatcatcacc ttcttccata actatattta tgttattttc tttgaagaat ctcttaatat
    14041 aagctggata ctcccctcta aaacttacat cagtaaaaaa atattgtatt ctatttcttt
    14101 ttaaagcaaa tactacattt tcagggtcgc aatcttctgg ataagatgta caatctgcca
    14161 tcatcatccc tatcatcata tctgaattta tttcttttcc aagcttctta gttaaagctg
    14221 tagccatgaa ttgatgatga atagcttgat atttggcctc ttctacatca tctacctggt
    14281 ctacacaaaa tgcaactgaa ttaaatgatt catgatacat taaatttatt tggtttataa
    14341 ctatccaata tttaacttta tctttgtatc tatttaatat taattctgca tatctaacaa
    14401 agaaatccac tacttttcta ttagaccatc ctttatattt taatactaaa ttcaaaggag
    14461 tctcataatg gagtagtgtt acaataggtt ccataccatt atctattact tcatttataa
    14521 gattatcata aaactttaat ccctcttcat taggctctaa atcatctcca tttggaaata
    14581 ttctagccca atttattgaa aatctaaaac actttaaccc tagttctttc aaaagcttta
    14641 aatcttcttt gtatgtatga taaaagtcta ttccattacg cttaggataa tacccattat
    14701 tatctttcat agcaaacttt attttatcta cagtcatatc agactcgtaa tgtttagata
    14761 tatctctatc tttatcatac atatgtacat cagctaagca ccaccctttg ccaccttcat
    14821 tccaagcacc ttctaattga ttagctgcta cagctccacc ccacaaaaaa tctttaggaa
    14881 acttactcaa tttaatcact ccttatttta tttatttaaa tcatttacta ttatttacct
    14941 tatattctat agactttact attcctacta aagacatagt tatatttaac tcgctgaata
    15001 ttgtcataag agtatcttga gcatgtgcaa ataaaagtga atgtgctctt ttttctccac
    15061 tagcttcccc ttgtataatg ttagtttgag ctctatgtgc ttctgtcata aactcttttg
    15121 ctttagctag ttttaaatca gcatttttat aatcaaacct ttctaaatca ttaagagcat
    15181 gtttaatttc tgttctagcc tcacctgcac gaagtattat ttgcattgct acttctacta
    15241 attcttcatc tattacatgt tcttcagttg tcatatttat aatttccttt caacatctaa
    15301 atttaagctt cttgactctc taattttagt tcttcttttt cataagcttt aaaaaatgga
    15361 taccatatca tgaatagcaa tactatattt attgctaaaa caataagccc tctaacatct
    15421 tgatttacca taaaagtaga tataccaaaa ggaagatacc ataaattaaa caccttactc
    15481 ggaattgtca caaatcccat actcattgat aagtaagtag ttattgatat tactactgtt
    15541 cctatccaca taggtaacat taaaaatggg ttccatgcta ctggagctcc aaacattact
    15601 ggttcattta tgttcattat agaaggaacg atacaggctc ttccgattgc ttttattctt
    15661 ccagctctag ataaacacat cattagcact agtggtaatg ttgctcccca cccacctaaa
    15721 gttatccaac ctgtttgaac aacttcataa gtatatatat tagttgcaag ccctcctgat
    15781 gctacagctg ctgcattagc tgcaattgca ctaagctgaa ttgcataagt tattggggat
    15841 aatacccatc cacttatacc taaagaatat aaaaatacta ctaagaagtt taatagtaca
    15901 aatccaggca atgtttgtgc tatatctgat actggtttaa atatcctaac tattatttca
    15961 aacatatcaa agttaagtcc ataaattagt ccccaaccta taaacattat tactgctata
    16021 ggtaacatac tatcaaacca cttacttaca aaatcaggaa gtgctgaatc ttcaccaaaa
    16081 aatgagaaag acccaaataa agacattatt aatccggaga ataatcctgc aactagtgct
    16141 acaagcattc cttctcctcc aaatctattg aagttaaata tagctccttc attcgtaatc
    16201 tctggacgaa tcatcattaa cattaatcca gcacctgcaa gaccagctac tacactcata
    16261 ctttcaaatt tcttattctt cattatttcc ataggtaata aaaatgctat aaatagtcct
    16321 aaaagtccaa atgtatattg atttattgga cttaaatcag gtaagaaact tatgtaattc
    16381 ttcaagacag aaactatagt tactaaagaa cctacaaata ccataggcaa aactttcatt
    16441 atagttgatt gaactgattg tacccatata ttcttagtta tcttatttac aacaggagta
    16501 aacttttcac ttaacaaatc tataaattta ttcataaaca atctccccat agatatttta
    16561 tttgtttaat attaattcta atgcttcttc taaagccttt acaccatcta atcttccata
    16621 tgctgtttta gatatacatg ctactggaac attatattca tcagcttttt tttgtatttc
    16681 atctttcata taatctaaat gtggtcctaa taataaacaa tctatttctg gtaaatattt
    16741 atctatctct gactcacttc tagcttttat atctgcttct atgccttttt tctttgcact
    16801 ctttctcata ctagttgcca taaaacctgt acttgctcct gagccacatg ccaacaacac
    16861 ttttatattc ttcataacaa tacctccagt atttttattc attaatttat ttcttaatga
    16921 atttttattt gatttgattg tatttatatt gtacttccta aataatttcc tttcacataa
    16981 atatatttcc taataatagg aaatcattta ctcttaacca gacacataaa tatatagtat
    17041 aatgtacatt ataatacgta cattcaatta tataaatatc atatcataca gattttattt
    17101 taaaactatg agagggaatc taatgaaaac atctaaaaaa gagttatttt taaacttcat
    17161 aaaaacgcaa catgattgga tagattcatc cacattagct aattatctta atgtatcaac
    17221 acgttcgatt agaaaatatg taaatgaaat taattctact ggtgaattta tattatcctc
    17281 taaaaaagga tataaagtaa atctcaataa taactgtcaa accaaggttg atagttcaga
    17341 aaatatatca cctgataaca ggttaaattt aattttaaaa gaacttatag ttaatagtaa
    17401 tggaattaat atatttgatt taagtgaaga attgtttgtt agtccagcta ctattgaagg
    17461 ggatatagtc aaagctaata aatttatagg aagttataat ttaaagataa agcaatcgaa
    17521 atttttattg aaactaattg gaaatgagtc tgcaaagcgc aaattaatga gctctataat
    17581 atttaaagaa acgggaagtg attttttaag cttatttgat gtacaaaaaa tatatcaaga
    17641 gtataatttg accaaactga aagaaaatat aatatatata ttaaaaaaat acaatttgtt
    17701 tataaatgag tacgctataa ataacatact tcttcattta atgataacta tagacagaat
    17761 aaaaaagaat aactacatag aatctgtcga agtagtaaac tacatagata ataataatga
    17821 tataagcatt tcaagagata tatcaaattt tttagagtct gaatacaatt taactttaac
    17881 tagtgcagaa ttatactact tagtatttca gttaacaaac aaaaccacag tcttaaatta
    17941 tcatgaaatg gatactaagt cattgccaaa ttatatagat gaacattttg ttaaattaac
    18001 taaaaaaata attaaaaatg tatatgactt atactttata gatttatctg atgatgaatt
    18061 tgttgtaaaa tttactcttc atgtaaaaaa tttaatcagt agggctaaaa ataatcaagt
    18121 tttgagaaat caaatacctc agaagttaaa agattcctac cctctgatat atgatatatc
    18181 tgtttatatt tgtaatcaaa ttcaaatttt agaaaacata gaaatagatg aagatgaaat
    18241 atcgtatata tctcttcatg taggttcatt ttttgataga caaaaattac tagaagataa
    18301 aattctctgt gcattaatta ctccaaatta ctatgattta caattcaaaa tagtaagaga
    18361 cctcgagaag agatttaatg aatccataga aattattcaa attttttcag atactcataa
    18421 tcttgatttt gacaataagg tagacatggt tattactact ttaccaatta gtgatagatg
    18481 ccctatacct tttgtttatg taaatcctta cctaaacaga aaagattatg ataatatcca
    18541 aagtaaattt attcagatta aagatagaaa aaatatactt actgtacaaa atcatttaga
    18601 aatgtacttt agtgaatctt tgtttatgaa aaacatatat ttagatagtg ctaaagatta
    18661 cataaaattt atgggaaata ttctgtacaa aaataaatat gtcaaaccca attatataga
    18721 tgatgttctt ataagagaaa aaatgtcttc aactgcattt aataacaatg tagcaattcc
    18781 tcatagcatg aaaatggatg ctttaaaaac tggagtatgt ttaattgtta acgataaacc
    18841 agttaaatgg ggtgaggaaa aagttcaaat tatagctatg atacctataa atgaaaaaga
    18901 aaaagaaaaa ttcaattaca tatttgaaag ctttattgaa atattatctg agtggaataa
    18961 tgttaaagaa cttactaagg cagataacta cagtagtttt atgaatagaa tagcttatct
    19021 tattcaaaat atctaaatcc attaatttat ttccaatata tgtttcttaa gttattaaca
    19081 caaaatagat aagctgtcac actaaaaagt ctaaattaat atgtgacagc tttttaccta
    19141 atatactttg ctttaatact acatattttt tgattgttgt atactagtta taagattgaa
    19201 tatttgtgtt gttgtagctg tcatattctc actagcagtt gctttatcca ttgcttcttc
    19261 tattgtcata gctctattta ctatagaaaa ataagcatct ataccctctt cattacattt
    19321 aactgcatct tctgttgtgg ctccagccaa acctattact tttaccccat atttttttgc
    19381 cagttttgca actcctattg gagccttgcc catagcagtt tgattatcca atctaccttc
    19441 accagttatg acaaaatctg catcttttaa ctcttcctct aatttaattt cttctaatat
    19501 tatttttatt ccagattcta attttgaatt taaaaatgct aaaaaagcaa aacctaatcc
    19561 tcctgctgct cctgctcctt ctacattagc tacatcttta cttaagtaat ttttaactac
    19621 tttggaaaaa tttctaagcc cattatctaa ctcctcaatt atttctgatg ttgcaccttt
    19681 ttgagaccca tatatgtatg cagctccatt tttcccaaac agcggattat ttacatcaca
    19741 ggctatttta aacttacatt catctaattc ctttaatcta ttttctattt ttatatgtct
    19801 tatttcattt aaaacttttc cacctaatcc tactaacttg ttattttcat cataaaactc
    19861 aaaccctaag gcttgaagca tacctatccc agcatcatta gtagcacttc cacctattcc
    19921 aactataaaa tttctatagc ctttatttat tgcttccttt attatttccc caacaccata
    19981 agttgtagtg tataaaggat ttctaagttc agtcggaacc aatgttaatc ctgatgcttg
    20041 agctatttct attattgctg tacttgtatt ttttaataat ccatatgttg catccacttt
    20101 tttagctatt ggtccagtaa cttctatagt ctctttttta ccattcatcc cttgaactaa
    20161 agcatctact gtcccttcac caccatcagc aagtggcaat atctttactt gcgcatcttc
    20221 tttaaccttc aatatcccct tttttattgc atttcctgcc tctattgaag ataaacttcc
    20281 tttcaaagaa tctatagaaa ttaatatttt catatttatc tactcctttt tatttactct
    20341 ttattttaaa tatataattt tataagctta atttatcttt tattttatac tctatatttc
    20401 actttgtata caacttatac ccttaatatt cttttatctt ataaaaaagg gagtgtctca
    20461 aaatgaactt tttagttcat gagacactct ttttgtataa aattaaatat atgtttatta
    20521 tttcaacaat gataaggagg attatctcta ttttaagata attccttttt agcactatct
    20581 aatcattttt agcactattt aaccaatata gttttaactt ggttcattag gcactctaca
    20641 tttacagagt tattatctat tgatatagat tctaattctt tttcatttag gttagtccat
    20701 ttagctgttt taatattttt gttaaatact acgttagctg attttgtttc ttctttagaa
    20761 ttgtaaaatc taactactaa tgcctcttct ttttcagcct tttttacaac acttaacaca
    20821 aggtccttat tagtttcctt aagtaaacta tatgaataat ctacttctat ttctgattga
    20881 ttcaatttca tagcattata tggtatttta ttataagttt gtatttttgt taaatactct
    20941 ttagccatat ttgcaacatt tgcttctagt gtagattttt catgagtgac tattgctaag
    21001 tctattttta tatctcctat catttgagaa tctggagtag gcaactttat tcctgatgga
    21061 cgacctgggc gtcttgccat ttcttcttta cctaggaatc ctacacttct aaataatgtt
    21121 atagctattg tgtcaaattt tttccctact atttcaaact ctcttgtact gtttgtaaga
    21181 acagctactc catgctcttt gtcagacaat ccaacgaatg ttagcattgg atatatagaa
    21241 tctggtcttt cactccattc ttcctgttgc caaacttcca tagcatcatc atacacactt
    21301 ctctttatat gtccaaactg attatcagac acagaaaact ttgaagctat atttgttggt
    21361 atataagttc taaccctaca atctcttgct tggttattta tattgaatac tacatttatt
    21421 atagctttat catttggaac atttataact atatcaaagt caataaatga atctgctcta
    21481 ttattttttc tagcttgtat atcttttggt acttttaatc tataatttat ctttatctca
    21541 ttattaaatc tatttttctt tatagtggaa tttgctacta tattatcgtt aaatattaat
    21601 ttctcatttg gaagtggtga aaaatcatac tcatctcctt catctcctcc attttccatc
    21661 aataatacat tttcaaaagt tttatttaat tttttatcag atatctttaa tgtcccgttt
    21721 tcattaactt gaatagtata aaagtcatta tctaccatgt cacatacttc ataagctttt
    21781 tctaacatat gttcaacttc ttttatcata tacgccttat accccattgc aggtatttta
    21841 tctttaaagt ttattgtata taccataaat gaatcatagt ttccataatg aacaatttga
    21901 cggtctatta atcctgcatc tattacttct ttatgtacca cttcaaaatc caacttatta
    21961 ttatctttat caaatatttc aaatgctttc attttagtta taacttgggc gttcactatt
    22021 tcattcctat tgtaaggcat taagttaaat gctactaact tatctaaagc catttcacaa
    22081 ctcattgcat caactattct tcttttgtaa aatgttatta attggtctac tttttcctca
    22141 gctaagaaaa atctattcat tatttcctgg tgaactttat ctgaacaaca acatccaatg
    22201 ctgtcatgag catgattttt cattatttct ttccaaatta actctattaa tccatgatga
    22261 tattcaaatc ctagagaata tgcaatagaa gctagtggct ccaatatgtt ggttatcttt
    22321 gattctattc tagcatttgc tgattttata tccattcttg ttgagaatat acttctatga
    22381 accctcatat atttcccatc taaaaattcc ccttttattg tatctatatc cttatttttt
    22441 tctagctctt taaatatgtt ttcatatcta cttaagaaaa attttctctc tggataacat
    22501 tcttttaatt tatatataac ttcaaatata ttcttttgaa ttggcatttg gtcatgtcca
    22561 tttggaagta ttatatggtc agtagttgca cccttatcaa gaaccggtaa atatttatcc
    22621 attcttgttc ttagctcatc ttcattcatt ggtagatatt ttcctattgc ataacctaat
    22681 ggaagtattt gaaccaaaac agaagaccca tcatcactct tccaattaaa ctctgtttta
    22741 ttagttcctt ttctttcaga acatcctctc caaaacattg agtattttat atcaaaccca
    22801 tttagtattt gcggcatttg agcactttga ccaaatgaat ctggtaaata tcctattttc
    22861 atatattctc caaattcatc acaatctttt attccataca gtaaatttct aagtatggat
    22921 tctccaccaa ctaccatttc atcagtttga gtataccaag gacctattat tagccttcct
    22981 tcttgaacta atttttttat tctctcctta cattcaggct taactgctaa ataatcttct
    23041 aatatagcag tttgaccatc catcacataa tatggatagt tatgattatt ttctaacata
    23101 tccattattt cttccatatt atttacaagt aatattctag attcctcagt tgagaagtac
    23161 cattctctat cccaatgcat atgaggaact atgtgtacat tttttttcat tgcttatcct
    23221 ccaaaactta tctgataata ttttctgtct aattaaaaat ccaataaaaa tactactgag
    23281 tttacacaat tttttaatat ttattttatc actataatta aacctttgat ttaaatatct
    23341 aataacgtat cttttaatgt atctttcttt gatttttttg tttcctagtt attattaata
    23401 atattgctga tattatagct ccaagtactg ccgcaaagaa ccatattgat gctataataa
    23461 atcctgacat acttccctct aaaagcgcca atgagaatat ccccgctcca ggcacttgaa
    23521 gacctatatt gaagaatgct actatagacc cagttatggc tgaaccagct actaatgatg
    23581 gtataacatg gattgggtcc tctatcataa atggtattgc accttcagtt attccagcca
    23641 atcctaatat ccaagtttgc tttcctattt ccttttctga ttctgaaaat aaatctctgc
    23701 ctaaaacagt agctattgta actccaaaag ctgagaccat cttaactgat gcaaatattg
    23761 cataaggtat aaaatttcca cttgctattg ctcctattgc aaatgtatag gctgctttat
    23821 ttattggtcc acctaagtca aatgaaacca tagctcctat tattgcacct agtagtattg
    23881 catttattcc agtcatccca tttaaccaat ttataagacc attgttaagt gctgctacag
    23941 gctttcctat tacaaataac atcaataaac ctactaccaa agaaccaact actggatata
    24001 cccaaaaact tacgaatcca gctaatgtgc ctttggctgg tatattcttc tttaaaaatt
    24061 ttactatata accagcaagt aaacctccta acatacctcc aagaaatcca ctacctatta
    24121 aatcagctgc tagacctgct gcaaatcctg gtccaagacc tggtttttca gctagtgagt
    24181 aagacatata tgctgataat actggaacca tcaatgtacc taacatccct ccagaaagct
    24241 ttcttaattg ccataaccaa gaaccttctt gattgtatag ttcttgtaaa ccaaatgctt
    24301 gagatgctaa tactgcaaat gcaagtatca tacctcctgc cacaataagt ggtattatat
    24361 gagatactcc tgtaagtatt gctgatttta catctttttt ccatgatgat ttttcacaat
    24421 atgatatatc agatgatttt tctatataat cacctttacc ttgtttttgt gctacttcta
    24481 aagcttcgta tattatttct tttgcccttt ttagtggagc tgctactgac gtttttatgt
    24541 ttggcaaatg ttcaaatctt tctgtatttt taggtgctac atctgttgca tatattattg
    24601 catctgcctt ttttattata tctggtgtat gagggtccac cataccatta gctccttgct
    24661 tttctacata cacatccaca cctatttctt tgccagcttt aattaatgct tctgctgaca
    24721 tatatgtatg tgctatacct gcagggcatg ctgttattgc taatacaatt ttttttgatt
    24781 cattttgaat cctaatctct tcttttattt gttctttctt ttcttttata tctaagttat
    24841 taaataactc tttcttagtt tttgaattta acaacatatt catataatct tcattagaca
    24901 gtctagtaac taactcagaa agtatttgta tgtgtgtatt tccaccttca ctttctggta
    24961 ctgctattaa aaatattagc tctactttat tatttgagtc tacgctttcc caattttcta
    25021 ttggtgtctc taaggtcata gctgcaaatg cagcttcttt aactgactta gattttccat
    25081 gaggaattgc aagccctctt tcaaaacctg ttggagataa agtttctctg tacattacag
    25141 cttctaaata ttcctcataa gatgttaact tgccttcatc atatagcttt cttgctaaga
    25201 actttattgc gtcttccttt gtttttattt tttgttttaa aaaaacaagg ttttcatttg
    25261 tgattttctt aaattccaaa attacgacct ccttaaatca tttaatgtta tgaatatagc
    25321 ataaaataaa tcatgaaaat gtttttttga aattatacac acaatttctt cttaaaaaat
    25381 aaggatttgt gtatttatac acaaatctct atcttaacca gtattgttct attattgctc
    25441 tcataatcat cataattgga accttatctg atatattgta cccatttact tgtttaattg
    25501 ttgaatagca atataaactt atatctgcaa tttgttgcaa ttgattttca catatatgtg
    25561 ttagagctac taccttacaa cccttattaa ctgctgtctt tgctgcttct ataacttctt
    25621 tcgtttctcc acttaaacta agcgcgaaaa acatatcttg ttcacctaat tttcctatat
    25681 catgaagtac ttcatgtctt tggctatagt aagttatatt atcatgccca cctctaattt
    25741 cttttactaa tatctcacat aaaggaatgt tttgacctat tccataaact ataatactcc
    25801 tagatttttg aattatatct gctactttat ttattttttc ataatctatt agctctaatg
    25861 tcttttttat atctgattca aaaccatttt ttggctcttt atcctcattt tttaactctt
    25921 cttttaaatt gttttttagt tgagagaaac catcatactc cattttttta catagcctaa
    25981 ctatggtatt tgggacagta aaaaatttat ctgctaaaga ttgtattgat aattttacta
    26041 tttcttcttt gttttttctc atatactcaa ttatcatgtc ttctgtatct gtaaatttat
    26101 actcatattt ttttgctctt tcataaaatt ccatagtttc tcctccatct attttattat
    26161 tcataagtaa aattcaaaaa tattagctaa aattttcgtt aatataatta taatttaata
    26221 tttttaattt attaatgcat actactcttt ttattataca taaaaaatgt gtacttccta
    26281 tattgataga gatgattcag gtttttattt tgatacatct ctatcaatat ttttttagaa
    26341 aatacacatt tttttcaaat acttattttt aattaaaact ttaggtaatc tattctttat
    26401 atataattat tagacttgaa aatatcttta aaatcaacca attcaatatt atttcttttt
    26461 aaccacattt taacttcctt atcacatagc attgcatgtt catatactct ctcttgaata
    26521 agagaagatg attttataag actataatct ataaatcctg gatgagtaac catcaatgtt
    26581 atatctttat ctaaatttat atatttttct tcaaataatt taacaaaaga tttatttaac
    26641 ttgtaaaaat cattctgagg atatttactt ggcatgctaa tacatgaatc taaataatag
    26701 ggacaacttg gtataccgta ttcttttgca gcatcacaca ttgctgatat tgatacatct
    26761 tcttctatac catgtatatc aatatactca ggcaatttac tcatatacaa cttgtaagtt
    26821 tcaatttgag cacaaacttc ttcatatact tcttcataat taaaaacctc tttacctgtc
    26881 agtagttgcg ttcttcttat tttgctagaa acaaattttc caccttcatc taacaaattc
    26941 tttatcttaa tatgttcttt acaacaaggt tctcctatta ctaaattaac atgtaaacca
    27001 aaacatacat catcattttt taccatttct atagcttgtc ttgaaaaagg catattcacc
    27061 atgattccag cacttgtaac aataccgttc ttataagaag atattatccc tagtgtaaca
    27121 ccttctgaat atcctatatc atctgcccta actactaatt tcttcatttt atgcatctgc
    27181 ctgctttctc aactcatcaa gttgcatttc catctcatcc tttactttaa caacatccgt
    27241 tcctatgatg atttgaactg atgtattact caatttaatt acccctttgg ctcctgtttt
    27301 tttaatatta tcatcattca aaatagtggc atccttcact tctaaacgta acctcgtcat
    27361 acaattatta agagtcacta tattttcata accaccacaa tttttaatta tctgcttagc
    27421 catatatgaa aaattaccat gagaaagttt taaattcttt tctttttcac taactttatc
    27481 acctatctct atctcaactt catgtcctgg tgtttgtaca ttatctttta ttattatata
    27541 tttaaatgtt acataatata atacaaagaa tacaactcct acaggtataa ctaaccacgc
    27601 tttatctcca taactaaaac ttaagacata gtcaattata cttgctccat taaatgaacc
    27661 caagcgagca cccagtaaat atagtacaac tccacctaga ccagaataaa aagaatgaat
    27721 tatatataac tttggagatg caaataaaaa cgaaaattct attggttcag ttactgttgc
    27781 cataaaagaa gtcaatgctc cagcggtcat tgcacctttt acttcttgct tctttttatc
    27841 ttgtgctgtt ttatatatag cataagaaat agcaggtatt gaaaacatca taattatgta
    27901 aaatcctgtc cagaagaatc ctgcatttgg gtctcctgcc ataaatctag gaatttctcc
    27961 tcttataact tctcctgttg atgttgtata ttcacccatt tcaaataata tgtaagcatt
    28021 taaaacttga tgtaacccag ttggtattag tgcacggtta cagaataaaa acaagaatat
    28081 tcctattgca ccagctcctc ccaaccaaaa accaaattga ttcattgcag cttgtggata
    28141 tatccataat aaactaaata tatatgataa aattaaagca accactggtg atagtgtaat
    28201 tacacatttt tctcctgcaa agaagcttaa aaatgctgga actttaactt tatatgtttt
    28261 attgtaagtc catgctatac aagctccaac aattatccca ccaaatacac ctgcatttaa
    28321 tgtatcattc aataactcta aatttgactt aaatatcata tatgcaacta cggctgcaac
    28381 cattacactt gtcttgtctt tagactttga ataagcacta actgctccta ataaaaataa
    28441 gtagtccaca tttgaaaata ctatattaga tatattagat atagctggca cattaagcat
    28501 atttggacca aataataatc ctaatatacc tgctgctggc attacagcta ttggcaccag
    28561 catagtcttt cctaaaagtg acagttgttg catcattttg tttaagaacc tcttcatatc
    28621 caatcccctt tctatttcgg tttaattttt tgtgaaaacg atttattcac attttatatt
    28681 ttaaatatta tatttgttta aacaaattgt caatattttt tattcatttt atttttatca
    28741 tttagtttca tatctaaatc cttttaagta tattgctata tctagcctat tttttaaatt
    28801 tatttttttt atttttaaaa tatgtttaac catgtatttg aaacaaccca caaaatagtt
    28861 caaatttatc ctttttatgg tttatataat tctattttca ctatacatgt ctaaacacat
    28921 ttgatataat ttgttcatat cagttcaata atattactaa agacctttaa ggagggatag
    28981 gggtatatgg ctaaaaataa attttcagag atatatgaag tattgaaaga ggaaatactt
    29041 gatggtaaat atacaagtaa tatgatgctg cctactgaac ttcaacttat tgaaaggttt
    29101 tcatgtagca gaaacactgt acgtagagca atttctcaat taaacacaga aggatatgtt
    29161 caaagtataa aaggcaaggg tgttgtagtg ttagaaaata gttgttctaa tgattttttt
    29221 ctaaatatgc acaatttcaa aggtgtagaa tcgattgtag aagataaaaa ggttaatact
    29281 gctactagtg ttctccactt cagcaaaatc ttaattgata ataagttatc taaaaagaca
    29341 ggttttaaag ttggtagtga agtatattac cttcacagac taagatatat tgataacata
    29401 cctaaaattc tcgacattaa ctattttcta tgtagtatag ttaaagacct tgatgtttct
    29461 atagcacaag gctctattta taaatatata gaagagtgta taggtacaaa aattgtttca
    29521 tccagaaaga tatttaaaat tgaaaaagct acagagcttg agttaaaaac gctacctcta
    29581 aatgattata attgtgttgg tgttattaaa aatagtgtat atactgatga tggaaagctc
    29641 tttgaatata cagaatcaaa gcatacacct gaaacatttg tattcatgga tgttactcaa
    29701 agatattaaa aaaattatgt ttaattaaac ataatttttt tacgtttaga cactttatcc
    29761 catactaacc tcactattct atatacaatt atatgtttat acaaataatt atttattgta
    29821 tattttagct ttaatattag tttaatactt gacaatttgt ttaaacatat tataattaac
    29881 ttataaatta caggaggtat caattatgaa aaattggtgg aaaaaagcaa ctgtctatca
    29941 aatatatcca aaaagtttca aagatagcaa caatgatggt ataggtgata taaatggtat
    30001 tatagaaaaa ttagattatc tgtattcttt aggtgttgat ttattatggt tgactcctat
    30061 gtacgtttct cctcaaagag ataatggata tgatatagaa gattattata atatagaccc
    30121 aaaatatggg acaatgagtg actttgaaaa actcttaaaa gaagctcata aaagagacat
    30181 aaaaataatg atggatatgg tcctaaacca tacatctact gaacataaat ggtttaaaga
    30241 atctaaaaaa agcaaagaca atccatatcg tgattattat ttctggaaag atgctaaacc
    30301 tgatggttct gttcctaata actggatatc cagatttagt ggaactgctt ggaaatatga
    30361 tgaaactact aaccaatatt atttacattt atttgaagaa actcaagctg atttaaattt
    30421 ggaaaatgaa aaggttcgtg aagaatgtta caaagtacta gaattttggg ctgataaagg
    30481 tattgatggt tttcgtttag atgtagtcaa cctactctcc aaaacacctg gattacctga
    30541 tgaccctata acagggccaa aaggagatgg aagaactcat tacgctgatg gtcctcgtat
    30601 acacgaatat ttacataata tgaatcaaaa agttttcaaa cctaaaaata tagttactgt
    30661 aggtgaaatg tcatctacta cacctgaaga gtgtatcaac tatactagag aaaatcgaga
    30721 agaactatct atggttttta gcttccatca tatgaaaaca gattataaag gaggcgcaaa
    30781 atggactaat gaaatgttct ctctggataa attaaaaaaa gcacaatctg actttcaata
    30841 taaaatgtat gaaggaaagg gatggaatgc tctattttac tctaatcacg accaacctag
    30901 agctttatct cgatttggag atgatagatt ttttcgtcaa gaaagtgcta agatgttagc
    30961 cataacacta tttggtctac aaggtactac atacatatat caaggtgaag aaataggtat
    31021 gacaaatgcc tactttacta aaatagatga atatgatgat gctgaaagta agaatgcata
    31081 ctatcatatg attaaatctg gtattgatga aaaggaggca ttgcttatac ttcaacaaaa
    31141 atccagagat aatgcccgta ctcctatgca atgggataat actatttata aggggttttc
    31201 taatcataaa ccgtggttaa aagttaataa tgacaatatt agtgtagaaa atgagcttaa
    31261 tgattctcgt tcagtatttt atacttatca aagattaatc aaatatcgta aacaatatga
    31321 tatttttaca gaaggtactt atagattgtt agatgaaaat cataaaaatc tatttgttta
    31381 tgaaagagag tataaaaacc aaaatcttct tgttataagt aattttactc atgataatgt
    31441 agtatataaa ttacctgaaa cttatttgaa taaattaaac tacacaatac ttgaaaccaa
    31501 ttacactaga gatataattg aaaataaaat ggaaataaaa ccttacgaaa gtataatgat
    31561 atattataaa taaagttatt tatataaaaa ttggtataac accagggtgt tataccaatt
    31621 ttactaaatt caaaataaca tttatagaat atctatagat tacaataatt atttgacttc
    31681 tacatcatta tatttctttg aatatacttt agtcatatag aaatatatag gaactacaat
    31741 tatcatccca cctaatcccc aaactatctt cataggttca tcaatacctg cttttatcag
    31801 taataatata cttataataa ctgcaaatat tggtataatt gctcctccag gtattctaaa
    31861 atttacatct ttatcagcat acttctttct caatattagt acagctaaac atgtaggaat
    31921 atattgaata aatctagata caacacttat ttgagataat gttgtgaatg aacccgacca
    31981 tgctattagt aaagttccta tgacagatac tataattgct atataaggag ctccattttt
    32041 attagttttt cttatagact taggcattaa tccttgttca accaaagcag cagcagacct
    32101 tggtgtaaat atggatgatg caatatttat acctccaaca gatactatag ttcctattgt
    32161 aattatatac ccacctatag gacctagcat cttaatggca gcatctgcaa caggagtagc
    32221 tgaccctgcc aagccattac ccaacacccc tatacttatt cccagtataa gcatatatat
    32281 tactgatact acaaacatta ccattaccaa tgctttaggt acatttttct tagggttctc
    32341 catatctttt gctgcaacag ctaaagattc aaatcctgta aaagcataaa ataaagttat
    32401 agatactgct acaaaagctg atgacgcact ttgacccttt tgtattatta caaatggggt
    32461 aaaattagaa ccctttataa agaataatcc tacagctata aataaaacta ttggtaataa
    32521 ttttcctata gtcatgatat tatttaagtg cttagattgt ttcattccca taaaatttat
    32581 aatcccaagt cctacaacta atataccaat tattatattt ttaataagtg gttctgctgc
    32641 ttgtggccat acagatgata gtgttacagc aaaaaaattt gccatagtag cccaagcaat
    32701 catactaact atccacttca taaatccaac ttcaaatcca acaaaatctc caaatgcctc
    32761 ttttgcataa agataaggcc ctccatcagt atcaaaccaa ctacctgctt cagcaaaaca
    32821 taaagcaata gaaacagcta aaagcataca aaaaactaat actaatatac ttgctggacc
    32881 tatatctgta taagcatcac caggtaaccc gaatatacct gaccctatta ttgcattgat
    32941 tcctaatagt acaatactcc aaaaacctaa cttaatgcct ttttccattg ttattccccc
    33001 caaagccaac ttaatatatt tatatttttt atttagatgc ttctttaact attgccataa
    33061 atattttttt catattatca taatctctag tcatcatttc tggatgccat tgtatcccta
    33121 ttacaaactc acttccttcc ttttctatag cttcaattaa accgtcttta gatgtagctg
    33181 aaacaatata tcctggtgct actttgttga caactaagtg atgaaaacta ttaactaaaa
    33241 cttctttttc tcctaatatt tcatataatt ttgtcccctc ctttatctgt actgtatgtg
    33301 taggtacatt agatagatgt tgctgattgt gtttaatata agcaccttca attaaagata
    33361 aatcttgata caaactacct ccttcagtta catttataat ttgttctcct ctacatatac
    33421 ctaaaactgg ttttttcata tctaaagcat gttttaataa tttcatatca aaactatctc
    33481 tctttggtga tattgccccc aacttattat gaggttcttc tttccatatg agtggattta
    33541 catcttgtcc cccagatagt ataagcgcat ctatattaga tacctgctcc tttataatct
    33601 catcatcata tacaatcggt acaatatatg gtatagcttt acacataact actgattgta
    33661 tataatcatt atttacataa gccctttcat agccaggaaa cattcctcct tcatcaatta
    33721 gtaaattacc tgatattcct attattggat atttcttttt aaccaatttc ttcacccctt
    33781 aacttatttg tgaacaataa aaaatgctac ttctcttaaa gagcaagtag cataccaaga
    33841 ttttatttat ttgaacatta taattcattt tagaaatact ttttttgaat acaagtaata
    33901 taaataactt tatttactta tattttattt taatcctgtt tagtttgact tttttaaatt
    33961 gttttaagta caactaatag gtaaaatagg catttagatt ttccttttta tcaacctatt
    34021 aaatactgtt taatatattt tttccccttc tatttaattc acttcccttt ctaccagatt
    34081 cacatattat caaatccagc tcttttaaca tattaagtat tccccttacc tcaccttctg
    34141 ttatactgta gcctaattca tttattgaat taacaatacc atttctacct atatttttat
    34201 taagattatt ggcatttttt aaacactcca ttactaactt tactttctct atagatgttc
    34261 tatcctttaa tatgcttatt tctttactaa aatcttcagt tatatttaat aagttatgag
    34321 gtaaatcatt tattaaaata acatcctcag aactcataat gttaatatac atagcagtat
    34381 tttttaattc tcttatatta cctggccatt catatgcatt aataatgtct tgaacacctt
    34441 gactaattgc tctcttcctt ttcataaaat gctttaacat aacttctata tcttcttttc
    34501 gctctcttaa aggtggtata tttatgggca atacatttat cctatagtat aaatctgctc
    34561 taaatctatc tccttttatc atttctaata aatttttatt tgtagccgca attactctaa
    34621 catctatatc tattacattt tctcctccaa ccctcattac ttgattctct tgtaatactc
    34681 taagtaattt gctttgtaag tatataggca tgtctcctat ttcatccaaa aatatagttc
    34741 cattgttagc ctgttcaaat aatccttttt tacctccttt taaagctcct gtaaaagcac
    34801 cttcttcata tccaaaaagt tcactttcaa gtaaattttc tggcatagct gcacagttta
    34861 tcgctataaa tgactgacta tgtcttggtg atgcattatg aatagactga gccataagtt
    34921 cttttcctgt tccactttct cccgttataa gaattgtata atcagattca gctattttca
    34981 tacctaattt cttagttctt tccatcttcg ttgaaactgt ttttatatca tcaaaagtat
    35041 atctagcaac ttgtcccttt tctctaagct tctttgatag gttttgctcc aattttctta
    35101 tataagttat ttcttgaata caaaaataat acccagtaac ccttccaaaa ctctctatat
    35161 tatacttatt tacgtttaag tacttattat taaattttat aacttcatca tgtgccttac
    35221 tcaaactaaa aatcttttta atattctcat tctctaattc atctatattt ttccctataa
    35281 tattactttg aatatctagt atatccctta aactattatt gcaaataatt atgtttccct
    35341 tatctgatac cattgccata cctaagtttg ataaatttat tatagaattt aattcatctg
    35401 cttttatagt aagttctttg tatttagtat ttattcctga atataaacta attatttcat
    35461 ctgtatactt aattagcctc tcagtaattt ctttatcatc cagtttgagt tttgctatta
    35521 tttgaataaa cgttgaacta tcaatatacc tatggcctaa atcaataact tcttctatat
    35581 attttggaac taaacttgac tctcctggcg ttatagctat cttaatattt ctatattctt
    35641 cattgggatt gtatggtata aaattcaaat ggtttattcc tatattgtat aaattagata
    35701 ttgtttccaa tacagtatgc ttattatcgt ttactactaa tacatctatt tcttctggca
    35761 atgaaaataa cttatatata tccttttgct ttatgctcct atttatagtt attatctttg
    35821 agacatcttc tacatatttt tttattttta atactctatc atctatcatt accaatatta
    35881 tgtcatcttt tattaattcc ttctctgtta ttttatttaa ataataattt tttatcatta
    35941 ctttatcttt aaatatcatt tctagatttt tatttaagaa acattctaat ttagacaacc
    36001 catcagttac tactgctatt gttttcattt gattactcct tttaaaatat actctactct
    36061 tttataatat acatctgatt ttcactttaa tagcacaaaa aattgtacat ttagtatact
    36121 aacatattcc tttattagtt ttatgtccct aaatatcata gttgcaatat ctatcatatt
    36181 tcttaaactt ctataaagca aaaattgtgc caaattaata attttcttaa ttttattcta
    36241 aagttcctca atagtattta cagacttagc acttatagct tataagtctt tatattatta
    36301 atctattttt ataggttaat tgtacaaatc atatatcact tttacctatt aaatgcatta
    36361 taaaaaagat ggaataacga ttcgtcactc cacctttttt gtaataaaaa attgtatata
    36421 taaacactat ttactactct tctaagtaca aacagtatct acatactata acctttcacc
    36481 attagattct attacttgtt tataccagtc aaaactcttt ttcttaatcc tagacaagtc
    36541 tccacttcca tcatcatact tatccacgta tataaaccca tatctcttgg tcatctcacc
    36601 agtagatgca cttactaaat ctatacatcc ccatggagta tatccaaata aatcaacacc
    36661 atcttctaca gcttccttca tttgctctat atgtgctttt aaatactcta tacgatagtc
    36721 atcatttatc actccattag attctttttt atcataagct cctaaaccat tttcaactac
    36781 catcataggt attctatatc tatcataaat ttcatttagt gcatatctaa gcccttcagg
    36841 gtctatttgc catccccatt cactagactt aagatatgga tttttcactc cacctaatat
    36901 gttgccactt gtattatctt ctttagaatc actacttaca catgtagaca tataataact
    36961 aaatgtataa aaatctacac aaccttcttt taaaatttct aaatctcctt tttccatttt
    37021 tatttcaata ttattttctc caaaatatct attcatataa gttggatatt cgccacgcac
    37081 ttgtacatct ccacagaaat aattcattac tctactatct ctttgagttt taactgcatc
    37141 atctggatta caagtataag gatacattgt tgcaaatatt tgcatacatc caacttgata
    37201 atttgaatct atttcatgag ctaatttaac agccttagca cttgctacaa attgatgatg
    37261 taacccttga aaacgaagtt gaggtatatc tacttggttt ataaaatctg tagtcccttc
    37321 atttaatatt ccttgtgata agtagcctcc cataggcatt gtagctgaat ttatttcatt
    37381 aaatgtcagc caatatttta cttttccttt atatctttta aatattgttt cacagtaatt
    37441 catatacaag tctattacct ctcttgaaac ccatccattg tatttctctg ttattgcaaa
    37501 tggtatttca tagtgagata ttgttattaa tggttctata ttatatttta aacattcatc
    37561 tatcacattt tcataaaatt taagtcctgc ttcatttggt tctttctcca ttccagttgg
    37621 aaatattcta gtccatgcta ttgaaaatct aaatacctta aatcccattt ctgcaaataa
    37681 agctatatct tctttgtatc tgtgataaaa atctattgct tcatgacttg gatagaatgt
    37741 tcctttttct ataactctcg ttattctttt acttactttt tgagaacccc cagtacacat
    37801 atctgaacaa gaaggacctt tcccatctaa attccatgct ccttcacatt gatttgctgc
    37861 tgttgctcca ccccataaaa aatcttttct aagtaccatt actctacctc caataattta
    37921 tttatagtaa atttatttta aaataaaaat acatatatct aatttttaat taataaaact
    37981 ctcttttaag ctaaaacagt tatcaattcc tgactattat ttacatgctt ttgtgtattt
    38041 tctataatat ccacataatc agaataatta gtaacaacta ctggtgtttc taagcaatat
    38101 ccttcttttg atatgctttc tatatcaaaa gttattaatg tctgaccttt tttaatctta
    38161 tccccttgat taacaaaaga tttgaaatgt ttaccttcca actgaatagt atttaaccct
    38221 atatgtatca atagttcaca accattatca gatataattc ctattgcatg ttttgttgga
    38281 aataaagtca ttatagtccc atcaaatggt gccactactt ttccttcttt aggtgttatt
    38341 gctacacctt ttcccaatat acctgttgaa aacgctgcat cttgtatctg ctccaaagcc
    38401 tttacttcac cttttattgg gctatatact acttctcttt tgtttttttc tacaacgcct
    38461 tgcccattat tatctttaga aatttctact atagaatcat ctttccaaaa aaataatgtt
    38521 aaaacaaatc ctactacaat agctactatt attgctatta tagttgcata aacacctttc
    38581 atatctccag ttgttggatt tatataatta ggtataccaa atattccaag tccacccatt
    38641 acataacttt ttacattcat tatccccatt acagctcctg ccacagcacc acctacacaa
    38701 ctatatatga atggtgtttt tcttggtaaa gttattccat atattgcagg ctctattaca
    38761 ccacatatcc ctgaaataac tgcaggtata cacagctctt ttatcttctt atcttttaat
    38821 ttaaagtaca ttgctgctac tactgatgtt tgtgcaaaag atggtacaaa tgctcccact
    38881 aatattgtgt caaaccccat agtcataagg tttgacatag ctattggtac taatccccaa
    38941 tgtagaccaa atattacaag cacttgccac aataaaccag ttaatacacc acataaaatt
    39001 ggtgaaaagt taaataaagc taaaaatcca tttccaacta tattagaagc ataggtagct
    39061 ataggcccta taaccataaa tcccaatggt aaagaaacta ataaaactgc cattggtaca
    39121 aagaaattct ttactacatc tggtattatt ttagcaaaca ctttctccaa cttagctgct
    39181 aataatacta ttataattac tggtactact gttgaagtgt agttcattgc tattactggt
    39241 aatcctaaga aagttatata tactggtgat tcaaatattg ttccagtaaa tagattgtat
    39301 aaaggttgac ctatagatgc tagagtatct aattgaatac ctgaatagca taaaactgca
    39361 cctataagca ttccaataaa tggttttaat ttgaatttct tagcagctgt atatccaaga
    39421 attattggca tatactggaa cattgcatct ccgattgcat ttaacataat atatgcacca
    39481 tcagtttctc catacaaacc caatgctgta aatactgcat taaatccttt tatcatacca
    39541 gcagcacaca gaactcctaa tattggttga aaaactcctg atattatgtc tataaactta
    39601 ttaagtaatc cttttggcgc atctgaatct gaattatcta ttgtatctcc tcctaatcca
    39661 gccaaatcgc acacaacttc ataaacatct ggaacatgat taccaataac tacttggtat
    39721 tgacctgcac ttttcattac agtaactata ccatccattt tttttagcac atcatcattt
    39781 gctattgatt catcttttaa tttaaaacgt agcctcgtta tacagtgaga taaactgcta
    39841 atattatcct tccctcctac attctcaatg atttcttttg atagttgttc atatcttctc
    39901 attttttctc ctccattttc tttttgaaat atgaaggttt ggccaactta atgtcaccac
    39961 ccaatatata taaacctaat tattaggatt tatatctgac ttaatacttt tacttaccac
    40021 tctttcaacg tgaatagtta agtataattt ttcctcatct gttaaatcgt agttgtattt
    40081 tttatgtata aattcatcca acttttctat gcatttataa gcatttttat atttaacttt
    40141 tattacattt agtaaatcat cttcattatt ttcatgtaaa ttattactaa caattcgttc
    40201 tgcaaaatac tttaagtgag taataaatct atgataataa actgaatctt catcaaatac
    40261 tattctgtaa tggtatttaa ccatatttga aatttcttgt attatttgtg taatctcata
    40321 tatagttttt atactttgat ccatttgtgc attaactata tgcagtgcta taaatccagc
    40381 ttcatcttct gatagtctaa tttcaaattt tttttctata taatccaatg cttctaaccc
    40441 tattttaaat tcactcttat aaaatctttt tatatcccaa agtaatacat tttttacact
    40501 tactccttca cgagctcttt ctattgcagt atacatatgg tctgttaaag aaatataaat
    40561 actatcattt aatcttttcc ctagtttttc tttagcatat aaaattattt catctgataa
    40621 ttccatatat cttataggaa tatctgctat tagttcttta aatttatcag atatatttgg
    40681 gtctgaaagt ttgaatatct tatcaatttt atcctttgat atatgctctc ctgtacgttt
    40741 tttataagct aaacctctcc ccataactac tatttcctta tgattttcat cagttgtaat
    40801 aattacatta ttattgagta ttttttcaat aatcattgtg atgccccctt tattagcatt
    40861 aagttaaaat aaatcttttt taattattta gaatatttaa aatgtaattt attacataaa
    40921 aaaaaaccta tttattacac attaaataga ctcccatgag tcctgagtaa taaataggtt
    40981 tagcttgtat ttaaacaatc actatccgtt tgtattttat tttaattatt atttattata
    41041 tgttagcaaa tttatttttt agtgtcaact ttttctcaat attttaattc taaataaatt
    41101 atataaattg catctgcaaa gtactatttt tataaataaa caaaataaaa aaggtattac
    41161 ttgctttaag taatacccat ttatttttaa tttaagtggt ttttcttaat ctcatcaagt
    41221 agtccatctt ctaaataaat ttctaatgca catcctacca ttgcctttac tgctttgtga
    41281 agtttatcat aagcttcctc agaatttgca taatttagaa attctttttc atgaacatat
    41341 accattggac ttatatctaa ggctatttct gtatacatag tagggcaaac ttgacttaca
    41401 ttacctatat ctgtagaacc tactggacca tctttacctt ctaatatatc tgttatacct
    41461 gactcaatca aattattctt tgttatcttt tgaagtactt tgttatttat tatgttgtca
    41521 aaagaatttt caaagtatct ataagaaagc ttagctcctg tcattaactc tgcacctttt
    41581 gcacagttta taactttttt agtcacttca tctaaataac ttctttcagc agctcttaca
    41641 aaaaacttac aagctgcttt ttctggaact atatttggag cctctcctcc atttgaaact
    41701 attccatgta ttctaacatc tgattttaca tgttgtctta gtgcatttat cccagcaaaa
    41761 gtaagttgta ctgcatctag tgcattaact cccatatgag ggtgactagc tgcatgtgct
    41821 gatacccctt caaaactaaa ctctaatgaa tctattgcca aagccttaca gtttatatta
    41881 ttaaaagctt ctatatgcat ttgataactt acatcaacat catcaaaagc tcctgcctta
    41941 actaaatcac attttcctcc tgttgtttct tcagctggtg ttcctattag tacaattttt
    42001 cctttaaaat tttcttttaa cttacttaat actatacaag ctcctagtgt agatgctgca
    42061 atccagttat gcccacaagc atgagctggt tctttattgt ctccatatcc aggaagtgca
    42121 tcatactcag ctaaaaatgc tatggtaggt ccatcattat ctcctaattc agctctaaaa
    42181 gcagtctcca tattacaata tggataaact gtatcaaatc catattcctt cattttctct
    42241 actaagtatt ttgatgatat gtattcttct tcccctaatt ctggattgtt aaatataaaa
    42301 ttacaaactt cttcaatttc ttttccaagt tcattctcta tctcataagc tctcttcttt
    42361 atactctcta tcataactta cctcccacta gaatcctaga tataatccta ttgtcaaaaa
    42421 taccattaca agaattgtta atactaccat taaaggaatt acgaatctaa cccactttga
    42481 ccaaggaata ttggctactg ctagtgctcc cattaaaaca cctgatgtag gtgttattaa
    42541 gttagttaca cctataccaa attgataagc tgtaactatc atttgtcttg atacatctac
    42601 taagtcgcca agtggtgcaa gtacaggtat tgtaagtgat gcaagccctg atgctgatgg
    42661 tattaaaaat gctattaatc cttctattaa gaaagttaaa tttataaata tagtttttgg
    42721 taatccattt aataaatttg ctgcagaatt taaaattgta tctattataa atccattttc
    42781 tgctattatt acgattccac gtgcaaatgc tatacataac cctgctgtaa ttaaatctcc
    42841 tgctccactt ataaatgaat taactatctc atcttgttta agccctgatg ctattcctga
    42901 tattatacct atagctgtaa atatcattgc aatttcagtt atgtaccact ctagtcttaa
    42961 aactccatat atcataatcg ccatacctat tgtgaaaatg gctattacca tggcttctct
    43021 ttttgtaaat tcctttatat ttgtatcact attaaccatt gattcttgat ttagtgatat
    43081 atcgtgtact aaagattttt tagggtcttt tttaacttta ttggcataca tcataacaaa
    43141 tcctatagat atagccatat atattataaa cataacaact ctaaactcta ttccagaacc
    43201 tggtactatt tgtgataatg cttgtgctat tcctacagag aaaggattcg tagtagctgc
    43261 tccaaatcca gctgttgctc ctataaatac tgtggccact gctgtcaatg aatcatatcc
    43321 catactggtc ataagtggaa taaatatcat ataaaaaggt agtgtctctt cactcattcc
    43381 aaacgtcgtc ccacctagtc caaatagaat cattgaaatc ggtattatta aaaattgaaa
    43441 cttcttcatt ttatttacag tatgagctat aacagcttct atagcccctg ttttattaac
    43501 tatcccaaaa gctcctccaa ctattaaaac aaatccaact acttcagctg catctataaa
    43561 tccgtttata ggtgctgtaa atacatcagt aattccttgt ggatttgatg gtgctttttc
    43621 ataagttcca gctacaacaa ctgaccttcc gtctatatct actctttcaa aatttccact
    43681 tggcactatc caagtaagaa ttgccattaa cactattagc gaaaatatta ttgtaagtgt
    43741 ctgtggcata gtaaactttt tcttttttaa actgctcatt tttaatctcc ccacttttta
    43801 aattaaatat tttattattg catcccttta tcctattttg tatagatgta aaaaagctca
    43861 acatggatac atgttgagct aaatgtgcta aatacaacat acctgcatct tccctttatc
    43921 ttgtttaaga tagctctcca tcaaaattcg ggataatttt gatgacagta ctgtacctat
    43981 taaatacagt cccagcacta gctttggaca ctagcacttc ggcacttact ccttttatat
    44041 tgcctcattg gtttccaacc tcttcaatat taatcttagt aaatgcaacc tctacctcaa
    44101 tctgttcgtt agatgaggta attcttatta atttttattg attcaaagta attgtagcat
    44161 aatatgtcga gattgtaaat attttcagta taataattct tactttatga tgtcattcca
    44221 tttttcacaa ataatttttt ctccatataa tttaaatact gtagtatatt ttaaatacac
    44281 acaaactttc cacaaatttt taattattat aataccttta taataaaaaa taaaagccat
    44341 gtaaacttat caatttacac agctttatat acattaatct actccaagta cagctttaag
    44401 ctttgatgct attgtaataa ctcgtggacc aaatataact tgaactgagt ttttcttttt
    44461 aacaactcca cttgcaccca attcatttac ccagaaacca tcttcataga atccatcaat
    44521 tgaatttagc tctaccctaa gtctagttgc acagtttgta acatttttta tattattact
    44581 tcctcctaaa ccttctatta tagaatgtac aagctttgta tctttatcat ctacttcctc
    44641 ttctactgca ttgttgtatg ctgttttacc agcatctaca gattgtcttt ctctatattc
    44701 tttcttacta taaagcttaa cttcttctga acttcttcct ggtgttttta tatcaaactt
    44761 cttaattgcc catgtaaata ttatataagt aactataaaa aatattggta tcaaaaataa
    44821 taatggtaac gcatccactt tttgtggttg taataaatta ggaaacatat ttctaaatga
    44881 ttctcccatt atagaaacat taaacatttc tgtaaataca tacgtcaatc ctgccagtgg
    44941 tacatatgct acaaaataca ataatggtgc aacaaatagg aatgtatact ctattggttc
    45001 cgttacccct accaacatgg ctgttactat tgctggtatc aatatactta ttgctttttt
    45061 cttattagca ttatcagcca acttatacat agctaaagct gctgctggta atattgcaaa
    45121 atgtattaat aaacgacctg ttgtgaagtt acgagttata taagatagtg tatctgggtc
    45181 tcccatttga gctaatctta tattattaac accctcgtaa attgctccac ctacttccat
    45241 tgttccacct actcttgtat attctatagg gaatgcaatt aagtgatgta taccaaatgg
    45301 taataatgct ttatccattg ctccaaaagt aaatgttcct aataatccac tagaacctat
    45361 ccaattacca agtttagata atgccaaccc tactggtggc catataaagt acattgctgt
    45421 acccaatact attgatgcta taaacatcat aatcattgaa aatcttggac ctgagaaaaa
    45481 agataatgca ctaggaagtt ctatgtcaac aaatttgtta tgtactgcac tagctacaaa
    45541 tccagctatt atagctccaa atatactaca atcatatgaa aagattccta agaaagaacc
    45601 atataaagaa cttcgactat atgcttctgc ctctcctaat ccagatgcca taaacgcttc
    45661 tgctgtaact gtatctgaat ttataccatt tgatgctaac aatgtactta taactgtgtg
    45721 cattcctata aataaaacaa ttcctcctaa tgcaccccat cctttttcct ttttagataa
    45781 tccaaaagct atacctgtaa caaagaatat tggtaagtat ctcattacca taaatcctaa
    45841 atcgtttatt actttaaaga atacatttat aaatgaccct tcttttatta gtccgctaag
    45901 ccctaatgca gaaacgtttt ccatatttgc aaaagctgac cctaatccta taaatactcc
    45961 tgctacaaca agtaatataa caggtatcat cattgctcca gaaagtactt gtaatttatc
    46021 cttcatagta ttatttcctc catttttatt gaaatgccag gctatataat gcatatatat
    46081 atacttctat tgctttgtat aatgtatcta tacttatatt ttcatttgcc tgatgtgaat
    46141 ttggattatc tccctctatt gtgggtccaa atgcaactcc attttttaac actcttgcat
    46201 aagatgcagc tccttttgta aaaagttcgg cttcttttcc aaaaccttgt ttatatgcac
    46261 ttgataattt atttattaat tcactatctg gttttacata cagtaatttt aaatccttat
    46321 atatatcaac tattaaattt tcttttttcc caatttcatt aattctatta gttataaact
    46381 cccaacttat acccttagga tatctaaagt ctattttcat attcaattca tttttctcta
    46441 agaagatact cattatacat attgtagaaa ctcccatatc tacatcttct ttatatatcc
    46501 ctaatttttt tccatgatta tcatctgtaa agtatgaatt taaaatatcc cttattataa
    46561 cgcctttatc atttagcttt ttaattttct ctaaatcttt tgctaaacta aatgcacagt
    46621 tatatgacct gtgtggattt ctcgataaag ccctttctcc tgtatatctt actaatactt
    46681 taccttcttc tagttcttct atctctgtat aataagctaa tgattctact aaatctttct
    46741 tatcatcagt tttaaaaaca gcctcaattt tgtcacatac aaatccatct tctttgtttg
    46801 actttatatc aactaaattg tgatttctaa atttatcttt atctattttc tttcttagat
    46861 tgaagtataa aataccattt tctccattta ctattgggaa gtttccatct ggagaaaatg
    46921 caaattttgg ctgttcattg acactaaagt aatgctccat acattcccaa gtcgtctctt
    46981 cagctccacc cactatcaat ctaatctttc tcttcggctt ttcattaagt tctcttaaga
    47041 aaagtaatgc atataaaatc cctatcaaag gtcctttgtt atcattaact cctctaccat
    47101 aaagatagtt atctttttga catactttaa aaggtttact cttccaaagt tctttattat
    47161 atattggcac cacatctata tgattaagca cccctactac ttcttctcct tctccatatt
    47221 ctatatgaat tgcatatcca tcaaagtctt ttacaacaaa atctttgtct tttgctattt
    47281 gaataagctt atcgaaggca tttcttattt ctatcccaaa tggttgattt atatctgttg
    47341 tactttcatc tctaattgaa ggtatttcaa ctaaacttct aatatccttt atgatatttt
    47401 cttgattgga ttttaaataa tcaataatta gttctttgtt tactatcatc ttaattattt
    47461 atcacccctt tatattaatt ttacaaagca tttcagttca tatctacatt ataaattttg
    47521 aatattattt tttcaatatt tcaaatactt ctctaaatta ccataatgat acggtaaaca
    47581 atcaacagat tatgacaaaa tcatactata cctattacta atattttaac ttatctatta
    47641 cgtctttttt aatgtaattt tattccaaaa atagatataa aaagatgtag ccttagctac
    47701 atctttttat atctattcaa cttccattat tatgctatta atatctacat ttccaggttc
    47761 tatcctatct tttactgttc tcttaacaga atcagcatca tccattataa ctacaggagt
    47821 tattatattt aaatcatttg acgttataaa atctaaatct acttccatta ttaaatcttt
    47881 agcttttacc ttatctcctg cctttacctt aacatcaaac ggctttcctt ctagtgacat
    47941 tgtctctaaa cctacatgta ttaatatatt tgtattatca ttactcttaa tcattattgc
    48001 atgctttgta tcaaatacat tgactatttc accatcaaca ggagaatata acttaccttc
    48061 atttggtatt attgctactc catctcccat catcttttgg gcaaaaactg catctggaac
    48121 atcttctaag ttcactaaat ttcccttaaa agggctatat attttctcaa tcttattttc
    48181 ttttttctta aatatattaa acataacaca ctctccatat tataaaattt tatgataata
    48241 tttttcttaa agcttttgct acaccatcat ttacattagt atcagttaca aaatctgcca
    48301 tagattttac ttcatctgta gcatttccca tagcaatacc aagacctgca aatttaatca
    48361 tggatatatc attttcatta tcacctatac aaataacttc tttgctatct atacctaata
    48421 tatcacaaaa ttttttaact gcaatacctt ttgaagtacc tttactcata acttcaaaat
    48481 ttatctttcc agaacttact acttctaaat cttcttgttt tttaaattct tcctttgctt
    48541 ctctcaaaga atctacgttt tctgagaaaa gtatacattt taaaatttca ttatcagctt
    48601 tttctaataa agtttcacaa cttgcatttt ctattatttc aattctgtct tcttctttat
    48661 ttttactatt tattaatcta tatggatgtt cttcacctat ttcattgttg gatataacta
    48721 ttttacagcc tttaaaatgt ttgtttaaat tatacttttc acctattgta tagattttct
    48781 ttatagcttc tttgctcaaa accttcttat actcatatcc cattaattta aaatatgtcc
    48841 cattagttga tataatatat atatcatccc ctaaaacact tgtaaaatat ttcatagcat
    48901 tataaggtct tcccgtaact aaggctacct tgataccctt ttccatagct tcttttagtg
    48961 ctttaatatt ttcttctgat acttcaaatc ctttccccat caaagtacca tccatatcag
    49021 tacaaattaa tttatatcgc atttgtatca catcctagta agatttttct aatatttcaa
    49081 caaggtcttc tttctttcca aaaggtaaaa atcctaaaac tggacagctt gttatttgtt
    49141 ctaattgttc ctttgttaca ttaaactcag acatttttgt cttaagtccc actctcttta
    49201 aaaaactttc tatttcattt cctaaagttt tagctgcttc atcatcttcc actttttcta
    49261 attcttggtt tagcattctc gctactttgg caaatttctc tatattatcc ttatagaact
    49321 tttctgcaaa ttttgggaac actatagcca aagcctcacc atgaggaata tgtgtaactc
    49381 ctcctattat ttcacttagt ggatgtggtg ctgctgctcc agcatttgcc agtgaacttc
    49441 ctcctaatgt atctgctact gctaactttg ttctgtattc tatattattt ttatcttcca
    49501 ttactttagg tagatagtta atgactaatt ccatagattt ttcagacatt atctctgtaa
    49561 agtcattagc atttggattt atataacttt caaatgcatg agtaaaagca tcaaatgctg
    49621 ttgatgcagt aattctctct ggcagagtta acataagctc tgggtcaact atacattctt
    49681 ttgagaaaca attttgatga aatattgtat ttttctcgct tcctctcgat attactgctg
    49741 cttgtgtaac ttggcttcct gttccagaag tagttgatac agctattaat ggtaattctt
    49801 tttcacttac cttatcgtat attttaaatg gatttgtata tgtactaaac atataatccc
    49861 aatctatttt ttctaaacca aaagtcaatg ctaatacttt tgcagtatct atacttgaac
    49921 ctcctccaac agctaaaaca acatctacag attctttttt agctaattcg aacccttcat
    49981 ttattaattc tacagttggg tttggctcta ctctatcaaa gtgtacaact tctatacctt
    50041 tttctattaa attcaatgta actcttttat ataacttgtc taatggctct acatttggag
    50101 tagtaactaa caaacatttc tttccatatt ttcctattat tctaggtagt tcacttaaag
    50161 ttccttgtcc aaaatttatt cttgatggtt gataaaagtt aatcatattt ttcctccctt
    50221 aaattattga cttcatattc aaattataca tatcatagga tttttaaggc aataccttca
    50281 ctgttaactc ttatttacta aagcttatgg caattattag acaataaatg ttttattatt
    50341 accatatcat tatgtaaatt ttattaagta cccatgttat aattttataa tcaatcttat
    50401 tgtataattg acttaataat aaaaatagag tatatacact tttttatgac aaatagctta
    50461 aaggagaatt aaattatgaa atatttgata gacagtgcta atcttgatga aattcaaaag
    50521 tgtttaaagt atggatttaa gggaataaca gctaatccat ctatgtatct taaagagaag
    50581 gtaaacttcc atgaatttat tgagacatgt actaatttaa atccagaaat attaactgct
    50641 gaagtcattg gaaataattt atacgaatta attaattcat gtgataagat attaaatata
    50701 aataaaaaca ctataataaa aataaatttc tctgaagttg gcttagaact tataaacata
    50761 ttaaatagta aaggtgtaaa aactgcatgt accttgattt tcaatgtaaa tcaagcatca
    50821 ttagctataa atgcaggtgc tgattaccta ttccctttta taggtagaaa tgatgaaaat
    50881 gggaatgatg gtataaaaaa ccttacagaa atatgtaacc ttatatcaat aaatggatat
    50941 aatactaaag ttgttgcagc ttctattaaa aatgtattcc acttaactca agcatctctt
    51001 tgtggatgtg attatgcagc tgtaacatat gatttattta aaaaagcttc ttttaaccag
    51061 cttacagtag atggtgccaa tacttttgaa agagattggt ccaaggttaa taaatagtaa
    51121 ctaagattta ggagggtttg cattgaacaa aagattatta gatattgctg aattattact
    51181 caacagctca gactatatta ctgtagatac tattgccgaa gaattaaaag ttagtaataa
    51241 aactattcgt aatgaccttg ttattttaga tgaatggctt ttagagttta atttatccct
    51301 agataaaaaa actggttcag gggttattat tctaggtaat gaagatataa aacttaaagt
    51361 tattagagat atcaatgata aatcaaattg tatatatgca tactctcctg aagatagaaa
    51421 aaggtatatt ctaagtaaat tatttattaa aaattctaag tttaggattc gtgatatttg
    51481 caatgaacta catgttagta gagctactgt tcataaagac ttggtagtta ttcaaaattt
    51541 tttgcaaaac tttaaagtga ctctagttag aaaaaccaat aatggagtat atttagaggg
    51601 aaaagagaaa gatattcgaa aagcagtttt tgaacttgct actgttaata aaagttatac
    51661 tgaacttaaa gaaatattat tctcgaactc taatgaatgc aaagataccc catcaacaaa
    51721 aatatttaag gagctattta actatgattt tgagaaatta tctcaaatca ctctacacac
    51781 cttacaagta gaaaaaagta accttagtga tgagtattat ataaacttct taatacatat
    51841 agctatatgt attaagagaa tttcaattaa aagatatatt aacttatctg aaggtttttt
    51901 tagagaatta ttagaacatg aatactttga aaaatcaaaa attttatgtg ctaacctaag
    51961 tgaagctttt gacgtagaat ttattgatga agaggtctgt tatatacttc ttcatataga
    52021 aggattactt aaatcaataa agaaaactac tactcttgaa cctcttaaaa atgaagaaat
    52081 tacagagtat aatctatctg aagcaatagc tgttcattgg ggaagtatac taaatttaaa
    52141 cttaaaggat gatttaatat taataaagtc tcttgctaat caccttaaat cagttctaca
    52201 cagaataaac tatggattta ctattacaaa tcccatatta aatgatataa agagaacatt
    52261 tccttatact tataaggctg ctaaagagtc taatactgtt atcaaacagt tgatgaatta
    52321 tgaagtcaat gatgacgaaa taggatatct tgctttatat ttaatatcag caatagatag
    52381 aagcaaatct cccctaaata ctattttaat atgtcactgt agtgatggtg tatctaattt
    52441 attagtccaa aagttaagtt ttgaatttaa tcagattaat atagttaaat ctatacctct
    52501 atcttctatt tcatttacga actttgatga tgtggactta atactaacca ctgctcctgt
    52561 agactttgaa cataatgctg aactgataaa tataaatgct cttttaagta agaatgatat
    52621 ttccagactt agtacaatta taaaaaaact atattctgaa aaaaataaaa tattatctta
    52681 taaataaaca aaaataggct tgtctcaaca tagagataaa cctatttttc attgtccaaa
    52741 tgataaaaat atatttgatt tcatagaaaa agtgcctcat gaactaaaaa attcattttg
    52801 agacaccttc attttttata tttaatcttt ttatttaagc ttttatattt tttaaacttt
    52861 tatatttttt aaacttttat atttaaagta aattttatta tttttattga gcgcaagcat
    52921 tatttatatt attaatttct tcagtattct taccaaactt tgtagttaca aatcctattg
    52981 tttttattat gagtaataag gatatcaatg aatatataga tatcataaaa ttcttactta
    53041 ttattccaga tgctactatt ataaatccat ctagcataaa taatataact ggcaacttca
    53101 gaaacttaat taccttatta ataagcattg caactaaatc agttccacca gtactacagc
    53161 cccttattat tgctatccct atagcagttc ctaccaataa cccacctatc actcctgcta
    53221 ctaatagatt gtcagttatg tgagtttgtg gtattacttt taagaataat ggacccataa
    53281 gagttatata taatgttttt attccaaaac ctttccctaa aaataataat cccattacta
    53341 aaagtggcat agatattcct aatgatatat tctcaacagg cattgataag aatgtgctaa
    53401 gtacgactga taatcctgtt atccctcctg gagctaatgt atgttctgca aagaaaaaat
    53461 taactgctaa tgccattaaa aatgctgata ttgttatcag taaataatct tttatcattt
    53521 cttttttcat ttaataacct cctatgatta tattgtttca gtataaaaaa tcaattaaat
    53581 tagtctagct tcctgtagtt tttcataaaa ttaatgcttc cttaaaacat taagaaagca
    53641 tctcttttat catagtttat tatcttattc taaaccaaac tttgtagctg cttcatctat
    53701 agcttttcta caattcttaa cacctgctgt tataccatct ttatccttga atattgctga
    53761 agataatatt acaacgtctg gattacattc tattaatggc tccatattgt ctgctcttag
    53821 tccaccatct attgctaatt cacacttagg attcttttcg tctatcattt ttctagctct
    53881 ttttactaaa tcaacagcac tctttctcca tccccagtta tcttttccat cagtttcatc
    53941 aacaccatga acgactattt gcaatctatc tatatcatat atagcttcat caacaaaaca
    54001 tagtggtgtg taacatccaa ttgttaatcc tactttcata ccaaattctc tacagtaatt
    54061 tattatgtat gcaagaggag cgcctaaaaa atgttctgct ggtattatca acaagttaca
    54121 tcctgctgct gctatttttt ctataaataa tctatcacaa tctcttgtgt atatatggca
    54181 ttctattggt ttatctgtca ctggtcttat tgcttctatt atttgatgac cacccataag
    54241 ttgcatattt tttaagtcat gcatatctgc tgcatctgaa tgtacgtagt ctacacctgc
    54301 atccattgct tctttaacta cggctgatat atgaccgtaa tttacatgtg ctaatcctgc
    54361 tgctattttt atgtgtttca tatttgtata cctccatatt ttactttaat tagtattaaa
    54421 tattagtttt tgatattttt taatccagta ttacttttat ttagcattac ttttattgct
    54481 gtatatattt cttttatgaa cttattctgc ttttgttaac tgtgaactta ttttgctttg
    54541 taaattaata ttacactgat atattttttt tcacaacact ttgactactc cttaaaagta
    54601 cctttatatt gtggtaaatt atagacatta attgtatata aaaagttagt tttttaccac
    54661 attcttatgg catttattgt ctatactaac tcaattgata tagctagttc atttaatata
    54721 taatcagact ataaatataa atttactcga taaaaagtac cattcataac aatattaatg
    54781 aaagggagat gtaaaatgag aaactgtatt attgcccaat ctggaggccc tacagctgtt
    54841 atcaatgctt ctgcagttgg tatttatgaa aaaaatttag aaactaaata ctttgataaa
    54901 gtatattttg gtatcaatgg gattgaaggt atattaaaca aaaatataat agattcagac
    54961 tccttaaaca aagataatgt attaaattta aagcaaacac cttcgtctgg tcttggttct
    55021 tgtagataca aactggctga ttataaaata aattcttatg attatgatag agttataaat
    55081 atactagatg aatatgaaat agatacatta ttttatatag gcggaaatga ttctatggac
    55141 acagtgatga aattttctca atactgcaaa aacactactt gtaaaataaa atttataggg
    55201 attcctaaaa caatagataa tgatttatta ggaactgaca actgtcctgg atttggttct
    55261 gctgcaaagc ttatttctac actaataatg gaaacatact tagatttttc tatatacaaa
    55321 aataatggta tatttatagt agaaaccatg ggtagagata ctggatggtt agcagcttct
    55381 tcttcactag ctagacttaa tgatactcca cttgttgatt taatatacct tccagaaatt
    55441 gatttctatg aagataagtt tatagaagat gttagaaaaa tatttaaaga aaaaaataag
    55501 gcttatatag ttgcatcaga aggtataagg gataaatacg ggaactttat atgcgaacaa
    55561 aaaacttctg ctcatgataa atttggtcat tcccaattag gtggagtaag caactatcta
    55621 aagaatctta tagtatcatc atcaattaca acaagagtaa aaaccttaga acttggtgta
    55681 atacaaagat gtgctatgca tttagcttca gatactgatt taaaacaagc agctgaagtt
    55741 ggtgcatatg ggatagaact atctaaaaaa ggatatacag gagttatggc tatcattaat
    55801 agaacatgca ataatcctta taagtatgaa gttagtcata ctgatatttc taaaatagct
    55861 aacaagacaa aatattttcc taaagagtgg ataaatgaag aatgtaatta tataactgat
    55921 gaagccctaa attatctata tccacttatt caaggtaaac cagatatcaa atataaaaat
    55981 ggactacctt atttttcaat tatttactag gaaattatta taatttgata tacactcaat
    56041 tctatcgctc ttaaatacct aagtggctgt taaataaaat tataaattgt tatatgacag
    56101 tcacttaatc gtttaattat ttaactgttt agttttttaa ttttttaatc ttttttaata
    56161 ctaatacgat ttgttgtaat aatatctcac acccattttc attttggata gattataatt
    56221 agttagacaa taaatatttt gtcatgatat tattatatat agtgatacaa atagaaatac
    56281 ttttatcatg actagaaaaa caaagattac acaaaggatt taaaaatcaa acaacttcaa
    56341 ttaaaataaa tggttaaaat aacttctaaa tcaactacat aaacaataaa agaacataaa
    56401 gtagttaaat ctacaattat aaaattggta agactataat aacttaaata aaattaaagt
    56461 taacaaagac tattaaagtt agcaaatatt cctctaccta aataattgct ttttataaag
    56521 agttatttat tttaatttat tttaattgaa atacgatttt aactagaaca atagattaat
    56581 gtaatattta atataaaata attataataa ttgaaaagga agtaatctat gatgaaaata
    56641 gcagtcataa caaccgaatt tttaaaagag tttgtagata attctataaa aaagttaaat
    56701 attaatgcag aaattgaaat atatatctat agagattttt ctcatgttgg agatttatat
    56761 ttagaaatag aagataggtt tgatggattt gcagttagtg gccccatccc caaaaaagct
    56821 attacaaaaa aggctggaac tattaaaaaa cctcta
//
LOCUS       DAMJWP010000006        55468 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.006, whole genome shotgun sequence.
ACCESSION   DAMJWP010000006 DAMJWP010000000
VERSION     DAMJWP010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 55468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55468
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.006"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            185..901
                     /locus_tag="PC700_000329"
     CDS             185..901
                     /locus_tag="PC700_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2579657.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIVDIMMPKMDGITLTMRLRENYNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVGNKEQKVESDDSVFAIGGLELNENTKEVNIDG
                     KYIKTTPIEFKILSLLMRNAGRVFSADEIYERVWNENAINTDTVMVHVRNIREKIEID
                     PKNPKYLKVVWGVGYKIEKI"
     gene            876..3209
                     /locus_tag="PC700_000330"
     CDS             876..3209
                     /locus_tag="PC700_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2579658.1"
                     /translation="MDTKLKKFKDKYIVTIIVFILMLSASLGMISQYSTIQAGAKGGA
                     KNPFEQESFVDSIYKGSYVLYHNMKEEQEGKMIQPSKLFINEETMNYIKNVSKEKDTY
                     DGSETYYTDDSINADFNENFENWEHFIKASGKNLKYYLVDKESNFEFFNDNKELGVLD
                     TTKNDASKENSDENQSKNTEMNDEERQKQITDIKENYRFYVVMNFDKDGKVNIVDSYG
                     VNQKVINQMLLSKAREDLMDTSLGGNSISYKLSPIKNITLVYAVPKVISKGDSVEYGY
                     GNTYFDDGIISYISNVENNSYYKISGMILKIILAIVVIVALAFPYRKSKELLGFDTLL
                     RIPLEILFIALAIIQGLVDLYPTVIISNTLSGNYVEYLINNDISAKMASILVSLMNLT
                     YWFVIFSIIFVFIILLKHILNVGIREYFTKHTVTGMFFVWLKKAGKTVINQIRTINLK
                     EKPNKTIVTILGINLLIIVIMCSMWFFGIILALIYSVVLFKILSDYSKKTIYEYNQLL
                     DVTKKISDGNLDVNMEKDLGFFNPIKDELGNIQTGFKKAVDEEVKSQKMKTELISNVS
                     HDLKTPLTSIITYIDLLKDESITDENRKLYIDTLDRKSQRLQHLIEDLFEVSKANSGD
                     VYLNIVNVDIVSLMKQTLLELDDKLADSSLIVKNNFSNEKIILPLDSQRTFRVFENLI
                     INISKYAMPNSRVYIDILETDNKVNIMLKNMSASEIDFSVDDIMERFVRGDKSRNTEG
                     SGLGLAIAKSFIELQGGKMNISVDGDLFKVTITFNKN"
     gene            complement(3526..6204)
                     /gene="mngB"
                     /locus_tag="PC700_000331"
     CDS             complement(3526..6204)
                     /gene="mngB"
                     /locus_tag="PC700_000331"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HDF2579659.1"
                     /translation="MKKTKVHVVPHSHWDREWYFTTSRSKIYLMKDFQDILDILEEKE
                     DFKYFTMDAQASLLDDYLKWRPQDEYRIRDLVQRRRLVIGPWYTQTDQLVISGESIIR
                     NLYYGIDRCNEFGEPMRVGYVPDSFGQSAQMPQIYRGFGIDSSLFWRGVSDDMVDTTE
                     FIWKGSDGSKVLAVQIPFGYYYGGNIPEDDSDLKEYLKDIIGKLKVKASTNNVYFPNG
                     FDQAPIRKNLPDILKKANEIDNENEYEISNIENYIDSVKNERNEFTELKGEFLNAKHM
                     RIHKSIFSSRSDLKIMNNKLENYITNVLEPLLTLSYSLGNEYPHLVIKDIWKLMFENA
                     AHDSIGSCNSDTANEDVYTRYKQARDLSINLLDLHMRLISTQIKNNNEEITLTVFNGF
                     PEVRNEVVEFDTYIPGQDFVIKNKNGEILNYIIKEKEDITNYILTQTIRLNPSKKIYI
                     PKTVYRAKIALSVKDIPPMGYTQLVFELDTKNKCEIETSNCKEIENKYYKITANKNGS
                     LEILDKESSKTYKNQAVIEDNGDDGDSYNYSPPRKDIYVSSLDSVSSVEVLKSNIQEE
                     MILKYSLTIPASLEERSIGKVSVKMPVTMKITLEPKEQIIKFNVNIKNNQALSHRVRV
                     LFNTEIASKFSIADQQFGIIQRPTVLEKDLALWKRDNYSWQEKPITIEPMQSFVTLED
                     GQRGIALITGCVREYQIVGDNLDTIALTLFRSFGYMGRENLLYRPGRASGEKIIATPD
                     AQLLKELNFDFGLYIYNNKFDEANVANTAKRFLTPIQIYEYADFLNGRLLFAFNDEKQ
                     IYENEFSLMNVNNSNFTVSAIKKEEKGDGIVVRIFNGMKNEDAKGSLNINKNVNDAYS
                     AMLDEIYDNNSKLKVNTKTVEVNSLSHCKVQTIVIK"
     gene            complement(6279..7385)
                     /locus_tag="PC700_000332"
     CDS             complement(6279..7385)
                     /locus_tag="PC700_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDF2579660.1"
                     /translation="MSKKNKSLLSELKGHLMTGISYMLPLVIGSSLVVAIPKLTALAM
                     GITSLDAYKNVSGFYHILYLMEQVGWTGIGLLNTVLAGFIAFSIGEKPAMGAGFIGGL
                     IASNTNAGFLGAVIAGFFAGYVCKFLKKKIKISGSASGMMPLIIMPLITVGLTGFLMS
                     VVLAGPLGNINTSLNAWVAEMCQNGTNSVVLALILGAMIGFDLGGPVNKAAWMAGNAL
                     LLEGIYLPAILVNCAIVIPPLGYGLATFIRKSKFSNALAETGKGNIVMGIIGITEGAI
                     PFTLTNPLKLVPVNMIGCALGVGLSALLGVHAIMPPVGGLYGFISIGSGWAYLVGAIF
                     GALVIAILSTTLVDFNEDETTENTEFDEIELDIM"
     gene            complement(7499..7807)
                     /locus_tag="PC700_000333"
     CDS             complement(7499..7807)
                     /locus_tag="PC700_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDF2579661.1"
                     /translation="MKIVAITSCIAGLAHTPMAAKALEQAASKMGHEIKVEQQGAMGR
                     VNTITKEEAQDADFILIASDQTISGMERFDGKKVIKVKIGIALKKPEAVLTKCIEAIS
                     "
     gene            complement(7810..8262)
                     /locus_tag="PC700_000334"
     CDS             complement(7810..8262)
                     /locus_tag="PC700_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2579662.1"
                     /translation="MVLQEKYIFIDKDLKSKEEVLNFIADSAYILGIATNKEALLKDL
                     LKRENEFPTGIKDGFAIPHTKSVNVKEASILYIKTTNELEWKTLDDSKVRYVFSLLAP
                     HKNEGNIHLKTLSKLATCLMEDDFTSEVKNASDASHLVKYITNKMKEE"
     gene            8496..10424
                     /locus_tag="PC700_000335"
     CDS             8496..10424
                     /locus_tag="PC700_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2579663.1"
                     /translation="MHKRLLTLFKLLNESDDKITCKTLSNHLKVSERTIRNDITSING
                     TLEKNGAIIKIKKGEGYYIDILNLALYQQYLALISDDIMDSSEIPDSPIERNQYILKY
                     ILYNNTYIKLEDLANSLYVSKFTILNDIKRIKPILSKYNLILVSKPYYGVKVEGKEID
                     IRRCISNNMINRNFENYIIGITDREIELFNNVDLIELQRVVLSEINKFNINFLDFNLK
                     NFIIHLAITISRILDGYCLDNVLDVVLTDFQSNTTVENIFNYIESKYTIIISKADRVY
                     LYNHFITKSSLLDNVSNRVDTKIIEYVEEILEVINNQYTFDLRNDSVLFDDLVLHFKS
                     ILNSKSYNLNKVNPLINTIKSNYPLAFEITLNAIEKVFKNSIYSLTEDEIGYVSLHIG
                     AGIERFFQNNIKCKNVVLVCGSGYGSSRLLEVQLNKVFHDKINILQCLSFNQFLASEL
                     SDVDIIISTIPLNHDSIPIVLVDLKLLKKDIENISKSITNNSHIYSNLLDNFFDKNLF
                     IVNPKIKDKDELIKLMCNKLTQSEIVFPSFAESVFYRESLSSTNIDDFLAIPHPMELS
                     SIRTKICISILNEPIYWSEDSTVKLIMMLAINKDDYIKINSIYDILLKIIHDNDIRDS
                     ISNCNDFDNFLSIIKSIV"
     gene            complement(10684..11445)
                     /locus_tag="PC700_000336"
     CDS             complement(10684..11445)
                     /locus_tag="PC700_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2579664.1"
                     /translation="MEYTVQKLSKLAGISTRTLRYYDEIGLLKPLKINSSGYRIYGQN
                     EVNKLQQILFYRELGINLENIKNIINSPNFDSLSALKEHHNKLLAKRKQIDLLIENVT
                     KTIALKEGKYTMTDIEKFEGFKEKMIDENEKNYGTEIREKYGKDIIDTSNKKLKNMSK
                     QDYENWQNLNIEIISKLKKAFKTGDASSELAQEVAKLHHKWLSYTWNTYSKEAHSSLA
                     QMYVYDERFTSYYDREQPGLAKFLRDAIVIYTRDK"
     gene            11664..12140
                     /locus_tag="PC700_000337"
     CDS             11664..12140
                     /locus_tag="PC700_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDF2579665.1"
                     /translation="MNLKRSNEKREEYRRMYSDKESFLSLIQNFTSVSIAKELTLKNI
                     ELETSFICEYKGKEVDIIYKVFSKSGKVSHYIVLEFQTEMDTEIVPRLKSYREQIWKS
                     FIMKKSLEEIEDKNFKLPKVIPVVLYSGPERLSRKKGILDIVESISEFSNNSSMKN"
     gene            12238..12390
                     /locus_tag="PC700_000338"
     CDS             12238..12390
                     /locus_tag="PC700_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate translocator"
                     /protein_id="HDF2579666.1"
                     /translation="MRNNKSKVLLGILCLATGMAVILSMLLPGWIWSALTALILIGCG
                     ALLFFC"
     gene            complement(12550..12738)
                     /locus_tag="PC700_000339"
     CDS             complement(12550..12738)
                     /locus_tag="PC700_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDF2579667.1"
                     /translation="MAKVCSVCDKGKVSGNQVSHSNKHNKRTWSANLRSVRAIIDGAP
                     KRVKVCTRCLRSGKIERA"
     gene            13099..13449
                     /locus_tag="PC700_000340"
     CDS             13099..13449
                     /locus_tag="PC700_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDF2579668.1"
                     /translation="MSAKVTNQYGNIEIDNQVIAQVVYRAAMESYGLVGLAYKSKGIV
                     ELLKGENATKGVRVEEVEEDAIAIELYVIIQYGTNISTVANNIIDRVKYVVEKMTGVR
                     VTRIDINVQGIRVK"
     gene            13468..15111
                     /locus_tag="PC700_000341"
     CDS             13468..15111
                     /locus_tag="PC700_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HDF2579669.1"
                     /translation="MIQYIDGKRLREMFISGANNLQNNKELVDKLNVFPVPDGDTGTN
                     MSLTISYALKELAKVENDNISDIGKALSKGSLMGARGNSGVILSQIIRGIAKSIEGKS
                     KLSTEDLAKAFKNGSDTAYKAVIKPIEGTILTVVRESGEFAIKTAKKEKDVVKFLSML
                     VKESNSSLERTPDLLKNLKEAGVVDSGGKGLVLIYEGMLASIKGDNIEIKNVDLDTNI
                     STSMDFAKSTTSTDDIKYCYCTEFILESSKVEDTKIRDIMMAYGDSLAVVGDDGVIKV
                     HVHTNDPGNVLQEALKYGQLLTIKIENMKLQHENTLLDVEEKKENDSEPLEEEKEFGF
                     IATSMGEGLANIFKDFGVDHIIEGGQTMNPSTEDFMNAIKHINAKNIFIFPNNSNIIM
                     AANQAKELSDKNIIVIPTKNTPQGFAALVTFNGELSEDENKEAMMNALNSVKSGQVTF
                     AVRDTVMNEIDVKEGNIIGIAEGNLLSAGDYVDEVTSNLIEKLVDEDTAIITLFFGED
                     VTESQANELRTSLEEKFEDVDVELYYGGQPLYYYLISVE"
     gene            15192..17249
                     /gene="recG"
                     /locus_tag="PC700_000342"
     CDS             15192..17249
                     /gene="recG"
                     /locus_tag="PC700_000342"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDF2579670.1"
                     /translation="MDLYKDVQYVKGIGPKKADKLNKLGIFTLKDLLYYFPRQFEDRN
                     NLKKIAQLEDGEKVTIKAVISSINTFSPKEGMTLTKIDVKDETGSAKLVFFNKSYIKN
                     TFRPGDSILVFGKVKKKFNNLELTSCELEYLTNSPKNTCRFMPVYQLTYGVTNKEIMS
                     IIRTVLEDKELIIQEYMPQRIIEKYRLCSIDFAVRNIHSPSSKESLKIALYRIVFEEL
                     LILQLGLFVFKSGRNKEDGIKFETSKDLKKIISALPFKLTKAQNRALDEIIQDMNLEK
                     IMNRLVQGDVGSGKTVVALLALANCVLNGYQGALMAPTEILAGQHYISLTESLKDFGI
                     NVGLLIGSLTKKQKDTVLEQIRNNEIDILIGTHALIEDKVEFNNIGLVITDEQHRFGV
                     MQRSKLSLKGANPDILVMTATPIPRTLALILYGDLDISIIDELPPGRQPIETIAIEKS
                     KRDRAYNNLVRREVESGRQVYIVCPLVEESEAIEAKSAVELVEELRAEYFHDLRLGLL
                     HGKMKSSEKDEVMERFKNKEIDILVSTTVIEVGVNVPNATLMIIENAERFGLAQLHQL
                     RGRVGRGSHKSYCVLIYDSKTDVCRQRMAIMEETNDGFKISEKDLEIRGPGEFFGTRQ
                     HGLPELKVANLFKHIKILKLAQQEARYILGEDNNLQLKENMALKKEIIDKFKDTLKEI
                     SLN"
     gene            17308..17862
                     /gene="rsmD"
                     /locus_tag="PC700_000343"
     CDS             17308..17862
                     /gene="rsmD"
                     /locus_tag="PC700_000343"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDF2579671.1"
                     /translation="MRVISGKARGLKLNTPKNEDVRPTTDRVKESLFNMINSYIMESE
                     VLDLFAGTGSLGIECLSRGAKSCTFVDISKESIDIVKSNVKKARVESESIILNLDFKT
                     AIDKLKLQNSKFDIIFMDPPYYKNMFIEAIEKIDNSNLLNEDGIIVVEHDTNDLFPDK
                     ICKLEKTRDKKYGNTTLTFYKMEA"
     gene            17864..18361
                     /gene="coaD"
                     /locus_tag="PC700_000344"
     CDS             17864..18361
                     /gene="coaD"
                     /locus_tag="PC700_000344"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDF2579672.1"
                     /translation="MENKPRKAIFAGSFDPITNGHLDIICRASKLFDELQIGVLNNPN
                     KKGLFSFDERVKLIEKSTSHLNNIKVVTFDGLLINYCQENGIGALVRGVRSGADVDYE
                     LQMAHMNRELNPDIETIILPSCTKYSFISSSLIKEVLLFDADIKNLVPKIVLEELKKK
                     TSGGN"
     gene            18365..18889
                     /locus_tag="PC700_000345"
     CDS             18365..18889
                     /locus_tag="PC700_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2579673.1"
                     /translation="MKIDLEMIELFEQLEEIFRSASTIPFSHKCTVDKDEVLAIINDI
                     RTLIPEEVTQAVWINKERNKIISQAKQDATNIVEQAKKEAERIQQEYQDNIEELKKNS
                     EDVVKAYVESSEPVVQADQRAKDIVDRAERIANEIRIGSIEYAEDVLSSVEYNLREIL
                     EEVKRDKAELRPKK"
     gene            19233..21152
                     /locus_tag="PC700_000346"
     CDS             19233..21152
                     /locus_tag="PC700_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2579674.1"
                     /translation="MKERAKKILDFIIKNNNVTSQELQERFNVSKRTIYYDILAINEQ
                     LGKSGNIKNVKHKFIFEGNLCDARKIISTEEDKFLDSDYRKTFILNKILLGEKISIEK
                     LTNEMLLSKNTVVQTITDVKKYLQTMGLRLEYKGKYKIIGDEYVIRELFLIIVQENVL
                     EINSISKEVSSFDTKGHIKLTDYSLLNLTKFVEFLNKRIRDGKTLYSYKYLNEAKKIS
                     YFSNCKELLCEEANENEQAYICTYISSLPSLNSEVKEDVVEEYVDKLIDKFEVNTAIK
                     LESKHEFKKNILRHLHSSYNRIRFKFPIRNPMLDETKYKHESLYKIIKSIIENEEEFP
                     VFEGIREEEIGFIAAYFGGYLRGSRDNGLRRNKVLLVCPNGLMVSKSLEIQLYKYIPT
                     IEIVGIVSIKQLKEVNVYYDYIITTIDIQNVNNVIVVNPLLTSSDVQLLMNKLISVKE
                     NEKYFNLELIIQAIRKNGVINNEEALKADLLNIIHKIDEGEMYQPMLKELINAERVNI
                     IKSVRDWKEAIKIASKPLLEDNSIEELYIENMIKSVEKYGPYIVLADRFALPHASSKE
                     GVNKLAMSLLIVEDEVDLLGKPVNIFMVLAAVDNTTHIRALASLSEMMYEEENVKLII
                     NGDKSSILELINKQN"
     gene            21165..21452
                     /locus_tag="PC700_000347"
     CDS             21165..21452
                     /locus_tag="PC700_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2579675.1"
                     /translation="MKILAVCGFGVGSSMVLKMTIQKALKQLGIECEVENTDINSARG
                     ADCDCIFTSQELLNELKGTCNVDVYPVKKYMDLAEVTEVVQTFLSDKNDKK"
     gene            21585..22844
                     /locus_tag="PC700_000348"
     CDS             21585..22844
                     /locus_tag="PC700_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDF2579676.1"
                     /translation="MLDFISNNILRNPSMLIGLIAAFGLVLQKKKASDVIKGSLLAAF
                     GIIIMETGTGMLVSSIAPINGAFQSISGGAVTKGLSDVTFTASYGGIIGLAMFAGLAI
                     HLMIARFTPIKTIFLTGHMLYWFPFIFVASGVEAGLNGGVLIAFAAVLSALYWSIMPW
                     LLRKYVFDVIGDDSFTLGHPSGCLALISGFVAKLVGNKEKSTEDINIPQSLSFFREIS
                     ISGGMAVFLMFIVVGIVIPGAFKDSNEFIGTYAIKQGLSFGAGLIIMLHGVRMLINQI
                     MPAFQGISEKVVPNALPAFDCPILFNYKPNAVLIGFLVAMLTSTAMILICNYFNVFNL
                     LLIPLVITSFFECGAAAVIGESQGGIRGAIIGTAIAAIFMVVLVGISAVIYSGTIQNW
                     ILIFGGNDLSLFGIISKFIGSLFGGIL"
     gene            22847..23587
                     /locus_tag="PC700_000349"
     CDS             22847..23587
                     /locus_tag="PC700_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF2579677.1"
                     /translation="MKFKYIDEIRRLIDIIENEEKVNMEKAVQILVEAIESKNAIFSF
                     GASHAGILTEELFYRAGGCVLINPVFAKSLMLDTAPITHTSKMERLEGYGTALADKTP
                     IKKGDVVIVHSVSGRNPVSIEFAMKAKENKAIVICITNLSYSKSVSSRHSSGKKLYEV
                     CDLVIDNHGQKGDACVEIDGLNQKVSPTSTVIATTVMNSIIAQATQELVNKGLKNPPI
                     FYSANIDGGDELNKKIFDEYKSVIHYEY"
     gene            23910..25220
                     /locus_tag="PC700_000350"
     CDS             23910..25220
                     /locus_tag="PC700_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDF2579678.1"
                     /translation="MKKENKMEGIKVGMLKKLCSLAMVAIMTTTLITGCSSGNGKDSK
                     KDGEKEKLVLWMPPVENNMKEVWDPILDKFEEKNNCEVDLQIIPWDNYSEKFATAISA
                     GEGPDVGYMYAEMYPQFIESGAVEDLTNYATKEDKEQSIYIKTSEMMGGMYGMPFQAA
                     NPGVLYYNKDILEKLGEKPPKTWEDFKRICKKATQDTDGDGKIDQWGLAQGWGAKTFG
                     NLNWNWYPYLWQAGGDIFNDDLKSVKFNDKSGLEAANFLKELQAYVPEDSLSKDSNEM
                     IESVFGPGKAAFTIMLSSAATSVFDESFPDLNWGFVTGLEDKKAATFGAVDHLSLMSS
                     AKNKELAYKLIQHMLSVESMTEFHKAIPRAPITKGEPYQGDERFKEMVENDKNVYRPL
                     VVGPHGVEIYEYLWKELQTMISGDKTPKQALDDAAKYSNDLLAQ"
     gene            25318..26235
                     /locus_tag="PC700_000351"
     CDS             25318..26235
                     /locus_tag="PC700_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDF2579679.1"
                     /translation="MENLVREFEENSKRKKTKLNIKPYMYILPLGIILVAFYVIPIIM
                     SIYFSFTKYNIISPATFIGLENYKKLFTDETLKVSIINTIKFTVVVVPCQTILSLILA
                     VWITGKGNSKIASFAKGAIFIPVLSSMVLIGMVWRALLNGEGSIIYQVLGAFGIESSK
                     LLGDSKTALPTLMFISMWKNIGYFMVIYISAIMNLPKHCYEVAKVDGATKLQEFIKIT
                     VPLLKPTTIMVVFLGSIWSLQVFDLIYTVTGGGPGISTMSIVMHAFNLNFKNFNSGYA
                     MTVANVLFLLIAVVSILQNKLVKRDNSDF"
     gene            26251..27060
                     /locus_tag="PC700_000352"
     CDS             26251..27060
                     /locus_tag="PC700_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDF2579680.1"
                     /translation="MKTARRLLSDGLLLFIACASLVPFIYMLIISLKITYNSYSLDIS
                     FSTVTLQNYIDIFTKKGFAQYFFNTAIVSFSGVLLNLVFSTLAGYSFAKMDFKGSDKL
                     FLFMIMTLIIPSQVTMIPLYIIMKHLGWINSYLALIMPIPTAFGVFIMRQAILGVPKE
                     LLESAKIDGCSDFRILIQIVLPLIKPALITLAIFTFMGAWNEFMWPLIATTKDAMRTL
                     TVGLSTLKTFQITNYGQMMAGATITFLPPFIFYLILQSKFVEGVSLSGIKG"
     gene            27117..28865
                     /locus_tag="PC700_000353"
     CDS             27117..28865
                     /locus_tag="PC700_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HDF2579681.1"
                     /translation="MEISYVLKDCTFKHNKYSVIEAETWKEKDLDIVVAKGEKFAFQI
                     MLKATEEFNCTIDKSSTISWKGLENRVRLALNVPNSLENNFSINILGYVQDDTKAFVN
                     DAILRDKDVLVEQYLPQTFWIEGQVPEDFEENNLNIGIDIFKSFGYEDEEKVCTIDVP
                     VKVRNVVLKPLDKSKFFLDLWQHPSCLARMYKVELWSDIHFEIVDNYLKELASLGEKV
                     ATVIVSDYPWAGQSCYKVYKNPSNLYEYNMVSVSKGLDGKIKCNFESMDRYISIADKY
                     KMSKEIDLFGLLGNWCAGEFGNPVEGYKDPIRVRYFDENDKVFKFINNTNDLKEYIGL
                     VLNHLIECGLWDRVRIIADEPNNPEVVKECIEFINSTVGTHQVKYKSATHDQNFLDRA
                     KDEIDDMSINLKLTIQNYKDIENLKKKINDKGGILTWFVCCFPEKPNSFLSSPFVENR
                     IIGWYTYYFGLDGFLRWDYNLWTEDPWKDSSYKFPIWKAGDMFFVYPGKDLKPVRSVR
                     MENLRFGIQDFELFTMLEKEKGREYIEVELMQELLNKKENAEIKGFGDIELGYSLDNW
                     GYDKVKKYVLELLDRV"
     gene            29128..30150
                     /locus_tag="PC700_000354"
     CDS             29128..30150
                     /locus_tag="PC700_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HDF2579682.1"
                     /translation="MFGGINIKRIGIIIMFFLSIAFIGFYLAIRGNSDTVKMQRGINI
                     GNALESPKDFPWDVKMSNKFFDDIKDAGFDTVRIPVRFSDYTSDSDNFKIDEEFFKKI
                     DKYVDYALDKDLIVVLDLHHFEEIMKEPRVHKEEFLKIWQQIANRYQKYDKKLVFELL
                     NEPKENLSSQLLNEYIEEAIKIIRKTNPKRTIIVGPYNFYQIDYLNELNVPKDSNIVV
                     SFHYYEPNDFAFQGNIYHNGFEHLSNITWEGTNEQMDYLKKRFDIVENWANKNKVKIF
                     LGEFGVTKEAPEASRRAWIKAVREEAEKRNFSWAYWELASGFGIYNQIEGTWDRDILS
                     ALIEKR"
     gene            30155..32461
                     /locus_tag="PC700_000355"
     CDS             30155..32461
                     /locus_tag="PC700_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF2579683.1"
                     /translation="MKKNSKKILITLTIITNIVYILWRIFYTVPKEEGMFALICAIIL
                     LFVEIMGMMEMFVHYYGMSNIEYPEKPVISEELYPHVDVFIATYNESVDLVRKTVNGC
                     IHMQYPDKKKVHIYICDDGNREEMRILAEKMGVNYITRTEREGAKAGNLNNAMQHTNS
                     PLIATFDADMIPMHDFLIATVPYFLKNEQAKKDGEKEEYEKVGFVQTPQSFYNPDLFQ
                     FNLHSEGRIPNEQDYFYRDIQLARNRTNSVIYGGSNTVISREALEEVDGFYTYSITED
                     FATGILIQSKGYRCYAIPEVHASGLSPTDLKSLIKQRERWARGCIQTGRRLNILFRRG
                     LGFWQKISYISSITYWYASIKRFVYIMAPILFSVFNVIVVKCTLLQVLVFWLPMYILS
                     SLSLKIFSQNIRNTRWTNIYETIMFQSLMPAVILETFAISKNKFSVTNKNKLEENRMY
                     KFLQGIPYFIYMVLSIIGILKMFVAIFKMSSMTYSVVLFWLIGNLFNLVMATLFISGR
                     QQLRKSERYIAEIDFKLKQNSYVLSSKTIDISENGFAFLLENPEYISPEEEFEVEFRE
                     KSGNEMYIANMKAKIVNVVEVNSKWKYAAYITHIEDSEIDNWMCIVHDRIPTLPMTIS
                     NQLGFFDDLQINVKKRIEKIRTLSRRSPRINMNFQMDIKNTGKLRIVNFNYQYVLLNF
                     ENKNIYPKEIALEINENIVLECDLCEGKIDERGLLYKVNNIDSIMQNFFLRDEMMDWI
                     LQNKTIPVSNPSEKKEKSIDEFEPMEYI"
     gene            32433..33548
                     /locus_tag="PC700_000356"
     CDS             32433..33548
                     /locus_tag="PC700_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2579684.1"
                     /translation="MSLNPWNIFRRDEMKTLKVFFCSAKYLILVFIFFINTTFISSAT
                     SDKILDLSFKKIETDLSSRITYEDTGVKIETDSSKSDKERYLYIYQNIKENWSMYNNF
                     YIEIQNKNKSSQKINLSIQSKNMLEFRLKEGSEIFLEDKNIIYSDKIKEGCIEVPGEF
                     EGKIYVNFNSLINEKSNVVLDSSMLSNIVSWGITFIPSDEEHNIVIIKKISLLSEEKL
                     SFLHNIKITGNEEVQIPVLGQSISQYEVLGLKRDSKIKYSLMGKQDNVSISQNGKLTL
                     SNKAEPGQIILQVNVDDKFKIGRKIALTESWSINKKDKDGVPYTLVSPEQSPTVQYMK
                     KINFMNNIITFVRILFVSLVIICFGIYLYWKKCSKTK"
     gene            33566..34984
                     /locus_tag="PC700_000357"
     CDS             33566..34984
                     /locus_tag="PC700_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HDF2579685.1"
                     /translation="MLFSSLIFLFYFLPITLVLYYVFRFNRTIQNMILLAVSLFFYAW
                     GEPKFVVIMIVSIIMNYIFGLLVDRYRESKIKVKVFLVLMCVYNIGVLFIFKYLAFAL
                     RNISELINTELTIPSIVLPIGISFFTFQGMSYVIDVYRRHGEVQKNPFYVGLYIAFFP
                     QLIAGPIVRYESVAEQILNRKETWNKFSIGTCRFITGLGKKVLISNNMAIVADYIYTM
                     NSQGEIAASLAWLGSIAYTMQIFFDFSAYSDMAIGLGLMFGFKFEENFNYPYISKSIS
                     EFWRRWHISLGMWFKSYIYFPLGGSRVANKDIMIRNMFIVWLFTGIWHGAEWTFVIWG
                     ILNFVFLIIERFIMFEKIENHNFIKHIYALLVVNFGWVLFRSPNLKEAYNYFKAMLGG
                     NGVIWSDYTYMFLKEYWVFFIFAFIFSVPIAKKINKFVVEQAKYSMIFNVFYPISAIL
                     LFFISVTYLVTGSYNPFIYFNF"
     gene            34999..36207
                     /locus_tag="PC700_000358"
     CDS             34999..36207
                     /locus_tag="PC700_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2579686.1"
                     /translation="MNRFFKVRLITAIMFLIILFAFSIFNICYSGKDMIQEIKNSVAE
                     KSSLKDTISSIDNTVNDKVIYKNTFIETYGYLQKLMGKNEFSKFSVVKDTSGKMHYTY
                     FAKAPNPVGVIADRVRKFSEEMEKQNTKLVYLMTPDKYIKGVTKFPKGIPYSYSNETA
                     DNFLNQLKKYNVDYIDFRENILKSGIPKDDLFFKTDHHWKVETSFWAFGELVEQLNKK
                     YNMNLDENHYYRDKENYNSIVYPKSFLGSMGRKTGILYGGIDDFTLIYPKFKTNYTYY
                     TDSKSQKFELKGRFEESLILSYPFNADLDLMDGQSDKYFTYLLGNRPLVKIKNIENPD
                     GLKVLFVKDSLIVPTAAFFSSVCSSIDMIDPRYYDGDILEYAKSHDYDFVFISVYPQN
                     LTKEFFPFCK"
     gene            complement(36288..37676)
                     /locus_tag="PC700_000359"
     CDS             complement(36288..37676)
                     /locus_tag="PC700_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HDF2579687.1"
                     /translation="MESSFLSQFLMITDFKTIFFIILLLGTFFIVRQFEKKKIKFSLR
                     TIYATVLGLILGIIIQAFAGFPNDTTQVTWLQEVSKWYSLFGSGFMDLLKMLVVPLVF
                     LSIIKVIINMKDNNLGTLTFKSLGMLLATTAIAAIVGIIVANIMKLGVGANMPTLVEN
                     QELKEINSLVDTLRGLLPSNPVMAMANGNIVATIIFATFIATSIKRLSKKHFETIKPF
                     IDFIEASYKIIVSVSMTVIKFMPYAVVALLANTITSQGISSIVSVLQFILALYISVAI
                     LFTIHLIFITLNGLNPITYIRNSTEALLLAFTSRSSLGTLPVTIESLVQNHGVDEGVS
                     SFTASLGANMGMNGCAGIYPALMAVTLANMAGVNMDMSLYVMLLIVITISSFGIAGLP
                     GTATMAVSVVISGVGLGSYFPLVGGILAIDPILDMGRTMINVNGAMVSSITVGKSLGK
                     IDKEVFGKKNIS"
     gene            38018..39352
                     /locus_tag="PC700_000360"
     CDS             38018..39352
                     /locus_tag="PC700_000360"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HDF2579688.1"
                     /translation="MRVVIIGGVAAGMSAAAKLKRIKPEYEVVVYEKTEIVSFGACGL
                     PYFVGGFFDDADELLARTPEKLREAGIDLNIFREVVEVDSESKKIKVKNIKTDEIYED
                     YYDKLMIATGARSIMPPIKNIKLKNVSTLKSLYDGEYLKKLLSNEDNKRVTIIGAGFI
                     GLEAVEACKKLGKDVDVIQLEDRILPQVFDKEMTDVLEEEINRHNVNLHLDEMVVELC
                     GEDKVEKVITNKGEIDTDVVIIATGVRPNTEFLSNTNIKMLKNGAIVVDEYGRTSVED
                     IYSAGDCATIKNIVSNENVYVPLATGANKLGRIVGENLAGREVSYQGSLSSSCIKIMD
                     MEAASTGITEKQAKDLGINVKSKFISDYNQTHYYPGRNKIYVKLIYDADTKVILGGQV
                     AGFKDAVQRANVLAAAIFGKMTTSQLGMLDLCYAPPFARTWDVLNVAGNVSK"
     gene            complement(39481..40398)
                     /locus_tag="PC700_000361"
     CDS             complement(39481..40398)
                     /locus_tag="PC700_000361"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HDF2579689.1"
                     /translation="MIDLTLLGCGGNVPMPNRFLSSVFINYKGRKILIDCGEGTQVSM
                     KLKKCGFKDIDLICITHLHGDHIFGLLGLLSTIGNSGRTSDLTIVGPVGIVDCIRSMR
                     NLVEYVPYTLKIIENPQGNFSLDNKVLRNLEISTISLEHSIECLGYSFNFRRNPKFDI
                     DKATKNEVPKILWNKLQEGQNIVLNSKQYTPDMVLGELRKGVKISLTTDTRPIESIPD
                     FIKDSDLFICEAMYGDDLDISKAVRNKHMTFREAANLAKLGNVKQLLLTHFSPSLDIP
                     SMYLENATNVFENTILGEDRLSLRLNFDE"
     gene            40606..41490
                     /locus_tag="PC700_000362"
     CDS             40606..41490
                     /locus_tag="PC700_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HDF2579690.1"
                     /translation="MKVLKKITILSLITLTLVGCSNGEDKKVVEGSKKIQNSKKDEQP
                     IIVHKDANILKDRYEPPKGFKRVDVEKGSFESFLRNTKLKNYGEKVKYYDGRIKESKG
                     IYDSVFDVDIGNRDLHQCADAIMLMRAEYLYQNKLYDEISFDFVDGFKAKYSKWAQGY
                     RISVKDSGSSWYKATEYSTSYESFRKFMDIVFAYSGTLSLEKELEPVKVEDMKIGDVF
                     IVGGSPGHASIIMDMAENEKTGKKVFMIAQSYMPAQQTQLLINRKNPNLSPWYSLDFE
                     GDLTIPQWTFKRDQLKRF"
     gene            41703..43544
                     /locus_tag="PC700_000363"
     CDS             41703..43544
                     /locus_tag="PC700_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HDF2579691.1"
                     /translation="MKIRRYLILVIIILITTSQSFISYGLERFEEITIFHTNDIHGRY
                     AEGDDHIQIGNLATLKKETPNSILVDAGDCLHGLPIVNMDRGKSAIELIKCAGYDYIT
                     PGNHDFNYGKDRLFELSKSANLGENTLKFLSSNIFENGKKVFDSNDIKEIDGIKIGFF
                     GLSTQETKNKTGFKNVEGLEFKNPIESATEQVNELKGKGANVIVAICHIGTNSLSKPN
                     SIDIANEVQGIDLIIDGHSHTKFENGKQVGDTLIVSAGQYLENIGEIKLTLDTNDVEN
                     IKIENKSVRLIGKEEALKYKLDYVVSKKVDEIKEQQEKILDKVIGATTNTLDGSYENV
                     RTKETNLGNLISDILLDKTKADISLFNGGNIRDTIEKGNITRRDIVDVFPFSNTIVTK
                     ELTGAQIKDVLEHGVKLYPEKSSAFLQVGGISYYFNPKQKEGERITSIQKEGKPLDLN
                     KKYVVATNDYIASGGDEFPCFSKEPILKEFGNLESAVIEYIEYKKEISKNVDGRIGIK
                     SKDELISKNHESDLILSENEKNINDNEENLSNNIKSSDSDKNKSLDKTSARESSNQGI
                     SKREAKEKSPKTGDLGFSNSIIIFIVSSTLICLLNFNQKELKDKKSK"
     gene            43848..44555
                     /locus_tag="PC700_000364"
     CDS             43848..44555
                     /locus_tag="PC700_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2579692.1"
                     /translation="MNNILLLEDDKSLNRGISFKLKKEGYNVFSAFGIEEAKNIFAKE
                     EICLIISDIGLPDGSGFDFCEEIRKKSDVYIIMLTALDEEVDIVTGYDLGADDYITKP
                     FSLMVLISKVNALMKRVNTVKNYTLLVCDDLFFYYIENKLIIRTDDKEEEIILSKTET
                     KLLKYLMENSMQTLTKEQLLESLWDSSGNFVDDNTIAVNIRRLRQKVEKNPSEPKYIK
                     TVRGVGYMWGERSIKKC"
     gene            44549..45859
                     /locus_tag="PC700_000365"
     CDS             44549..45859
                     /locus_tag="PC700_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2579693.1"
                     /translation="MLKNMNLKDGLSVKDDYIMRRVIVYSVLSVLIVTLVFILFEYSR
                     LHKLYEHQIKNTQNIAGILIEKYPNDEIDIVNSIYNSDYSKVSTGEKAFKKFGYGLEN
                     KMSNDKNFSLYLKSFFKESFFIFLMMFIVVIGIIYYFIRYINKRLSKIYFIVDKMAQE
                     NYLKDTTESEETGYLVEYNEYFKEGTFSKINNCLYELNRSLKIKFIKLEKEKESVKSL
                     VTDISHQLKTPLASLKLYNTLLIEEKLEEDEANEFLLTNKASINKLENLINSLVNISR
                     LEISMINIKKEDNDIKNTILNAVKSVTPKAKSKSITLNLNEFNSRIIPHDKKWTEESI
                     FNVLDNAVKYTQVNGEINISVEETANFFKIIIEDNGIGIEKNEFNNIFKRFYRGSADE
                     IESVEGSGVGLYLSRKILEEQGGNIIVSSKVGIGSKFSLFLTTM"
     gene            45976..46659
                     /locus_tag="PC700_000366"
     CDS             45976..46659
                     /locus_tag="PC700_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2579694.1"
                     /translation="MKILYTENLSKHYGKGENLVRALDNVNLEINEGEFVAIIGKSGS
                     GKSTLLHMIGGLDIPTSGKVYIDNKNIFTLKEEELAVFRRRKIGFIFQSYNLIPSLNV
                     WENVVLPIGLDGREVDESFIKELLKSLGLENKHDVLPNTLSGGQQQRVAIARALATRP
                     AIILADEPTGNLDSKTSDEVMSILKSMSKKYSQTLVMITHDDSIAQMADRVIFIEDGR
                     VSKVGDKND"
     gene            46652..49039
                     /locus_tag="PC700_000367"
     CDS             46652..49039
                     /locus_tag="PC700_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2579695.1"
                     /translation="MINTTKIAKSNLKQNKSKSILIIITIILSTLMLSSIGIYIVDAG
                     AYQKENTIKYSGNYQGILANVDEKQADILSNHADVELTGEMNGVGVEKLEDDSNISLA
                     YMNEDALKLNSFELIKGKLPTKENEIVLDSGALKALGYKNKDLGEKIKISYNDYKNDK
                     KIEKEFVISGILKTSEISEAGKYYYAIISELYMRNTRDMSQEDFNIYVKFNDKSNLSI
                     EQAKEKLDKIANDIGLDTINTAVNENYINALKPDMETIMGGGFVGLVIVLSSILVIYN
                     IFYISIVTKVQEFGKLRAIGATKKQIKNIVFKEGFILAGIAIPIGIILGYVLANIIIK
                     SFMDIDVKSSRLPVILSVVVISFISVVLSLLKPMKVASKVSIVDAVRYTGNKISNKSK
                     RKGYKNINLKRLSHANLERNKKRTYMTLASLILSGTIFITVSTALESFDAEKMAREHF
                     PYDIEVRLSGYEMNSDKNPKNNLNILQMDNPLSKDFFNQIKNMEGIKRIESARSVKIG
                     MEDYDVEFKYDLLQSINENDVKSLNKNLIDGKINLERLQTGDEIVITHVDTAKEMGVK
                     AGDKIRLTLYDGDKKIKKEFKVQAIAMGVPSFGIGKDFIDRTLKYDSTSSLGIYTEEG
                     KYQEIKDSIKKIAKNNGFLETDFIDSRIESNKAVISFIKIMGYTLTGIIGVIGFMNLV
                     NTMITSIVTRKKELGMLQAIGLTNKQLVKMLNSEAMYYTSGMMIGSILFGGILGYIAV
                     IFLKKTGLSYATYSLPIVPILLMIVCILIAQFITTYLIGRSFNKESLIDRVRYSE"
     gene            49318..50460
                     /locus_tag="PC700_000368"
     CDS             49318..50460
                     /locus_tag="PC700_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDF2579696.1"
                     /translation="MNKKINYAPGPTETRENVRLARAEKTTNPDIDIDFVEFYKKTCE
                     KFGNIVGTKNDVYILSGEGILGLEAACASLTEKGDRVLVIDNGIYGEGFKDFVTMYGG
                     EYVLFSSEYTKSIDIDELKAFLDEDSNFKYATVVHCDTPTGVLNDVSKICPLLKEYGI
                     LTVVDSVAGMVGERLSVDESKIDIILGGSQKAISAPAGLTIVGISQDAKNCIKNRKTD
                     VIGFYCNLNIWEGYYEKKYFPYTMPISDIMGLDKALDNILEEGIENVLDRHEKIAYSV
                     RKSIEEYGLELFLEEGYSNTVTAIKIPESIGALKLTDYMLKNYNTLVATSLNQYMDTI
                     LRIGHMGENANLNKIEHVLNVLDKSLSALGFKGNENLLNLFNKYYF"
     gene            50479..51003
                     /locus_tag="PC700_000369"
     CDS             50479..51003
                     /locus_tag="PC700_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF2579697.1"
                     /translation="MEVTAKQKFTTKDMVETSLLIALVFIATKFINIRLPISINGGLV
                     HLGTAMLFISAIVFGSKKGALSGAIGMSLFDLISGWTLWAPFTFIVRGVMGYLLGKIA
                     WANGNNGNNFLINVIGICVSSIWMLFGYYITEVILYGNFIVPLTSIPGNLMQVLIGLI
                     IALPISKVLKKCIK"
     gene            51103..51921
                     /locus_tag="PC700_000370"
     CDS             51103..51921
                     /locus_tag="PC700_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2579698.1"
                     /translation="MLSVTRYSIKNVRLKPWVKFIWYFETKSNILLNNKLLPTDSIDI
                     ILNLSDVMEYKIENQDYTASNMHFNGIRDKHGFIIQHGNIRVIGISFYPFGLYPFLKI
                     PISEFNRQIVDLEAVSELFAKKLEESLNPTQSVEKIVLYLEEMLLSILEEDLISNKSV
                     KLLNSFIYDNRYSNIKAFCDNTNINIKTLERICLKYTGYTPKILKRIYRFKMASSQLI
                     YNYKDNELFDFIYENEYYDQAHFIKEFKKFSGTSPIKFIGENKTIKENIKYNYL"
     gene            51999..52310
                     /locus_tag="PC700_000371"
     CDS             51999..52310
                     /locus_tag="PC700_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDF2579699.1"
                     /translation="MKIITEIVEFNVEENLSKEEFIEIVNDLELKFHSIQSGFIDTEL
                     LYDEKSQKWIMIQHWESIEDLKSASSKMFKDSSTEKFRKALKNQTVKMNIIPQIKSWK
                     L"
     gene            complement(52613..54616)
                     /locus_tag="PC700_000372"
     CDS             complement(52613..54616)
                     /locus_tag="PC700_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HDF2579700.1"
                     /translation="MKSQKRFKILDIDVYLQPFESDIKKRMKHYEDMKSKTLKNYKDF
                     SSFANAHLYYGFHQTNDGFFYREWAPNADSLSLIGDFNNWNRKSHPLRKIGSGNWEIF
                     IPGQNSLPHKSEVKVQVTANGKTFDRIPLYIKRVIQKDFGGFNGQIWQPKTPFIWTDN
                     NFDLKNITSPLIYECHIGMSTESESIGTYNEFTEKILPKIKKAGYNTIQLMAIMEHPY
                     YASFGYQVSNFYAISSRFGTPEDLKNLINTAHSMGIAVLLDLVHSHAVKNTLEGINEF
                     DGSEHQFFHSGSKGNHPAWGTKLFNYGKPEVIHFLLSNIKFWLNEYHFDGFRFDGVTS
                     MLYHNHGLGVSFDSYEKYFSMNTDIEAITYLQFANELIKEIKPNSISIAEDMSGMPGM
                     CIPIKDGGIGFDYRLAMGVPDFWIKTISNLSDEDWDLGKMWYELTTRRPGEKNIGYCE
                     SHDQALVGDKTIIFWLADKEMYWNMEINSNNHVINRAISLIKLIKLITFSLAGEGYLN
                     FMGNEFGHPEWIDFPREGNNWSYKYARRQWSLSEDNNLKYKQLLNFDKSMLELTKYSD
                     IFIQNSHELIHMDNDKKILVYSKGKYLFIFNFHPNLIQSVDISKFQNTNYKTILNTDM
                     VEFGGNTKKENLRDYDVYFSSTPPSSKMPIASRTAIVLLDNGI"
     gene            complement(54769..55407)
                     /gene="recX"
                     /locus_tag="PC700_000373"
     CDS             complement(54769..55407)
                     /gene="recX"
                     /locus_tag="PC700_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDF2579701.1"
                     /translation="MGIITKIEQQKRNDDRVNIYVDDKFFIAIFKELVYTFNLKKGNE
                     INEEELKPILDDEMYIKAKNKALNILSHADQSEKKLKEKLSSEFDENIIERVIDFLKS
                     YNLVNDSVLAQKIVNTNVNLNKCGKNRIKQNLYNKGINRSTINEAVSELDKDTEFENA
                     MYLAKKRYERVKKEDKKKIYQKISQHLSYKGFDYDIIKRVLNKLLNFDEYDI"
BASE COUNT        21291 a         6226 c         9155 g        18796 t
ORIGIN      
        1 ttaagtatat aaaacaaaat gaaaaaatat taagtttaaa ctagaaggag gtttctaaga
       61 tggacattaa atacaaataa ccagaacaaa agatataaat atatatataa atggaaaaac
      121 ttctatcata ttgtataatc atatatgtaa agaaaattta taaattaata taaaggagat
      181 taaaatgaat tcatataata ttttggtggt agaagatgaa aaggagatcg cagatgctat
      241 agagatatat ctattaaatc aaggatataa tgtttttaag ggatacaatg gattagaagg
      301 tcttaaggtt atagaaaacc aagaaataca tttagctata gttgatataa tgatgcctaa
      361 aatggatggt ataacactta caatgaggct tagagaaaac tataattttc cagtaataat
      421 gctatctgca aaatcagaag aagttgacaa aataatgggg cttaatatag gagcagacga
      481 ttatgtaaca aaacctttta agccattaga actattggct agagttaatt cacaacttag
      541 aaggtacact aagtatctaa acatggttgg aaataaagag caaaaggtag aaagtgatga
      601 tagtgtgttt gcaataggcg gattagaatt aaatgaaaat acaaaagaag ttaatattga
      661 tggaaaatat ataaaaacaa ctcctataga atttaaaata ctaagtttac ttatgagaaa
      721 tgcaggaaga gttttttctg ctgatgaaat atatgaaaga gtttggaatg aaaatgctat
      781 aaatacagat acagttatgg tacatgtgag gaatataagg gaaaagatag aaattgaccc
      841 aaaaaatcct aaatatttaa aggtggtgtg gggtgttgga tacaaaattg aaaaaattta
      901 aagataaata tatagttact attatagttt ttatacttat gttatcagca tcattaggta
      961 tgattagtca atattctact atacaagcag gtgctaaagg tggagcaaag aatccatttg
     1021 aacaggaaag ctttgtagat agtatatata aaggaagtta tgttttatat cataatatga
     1081 aggaagaaca agaaggtaag atgatacaac catcaaagct atttatcaat gaagaaacta
     1141 tgaactatat aaaaaatgta agtaaagaaa aagatactta tgatggttca gaaacttatt
     1201 atacagatga tagtattaat gcagatttta atgaaaactt tgaaaattgg gagcatttca
     1261 ttaaagcgtc aggaaagaat ttaaagtact atttagttga taaagaaagc aattttgaat
     1321 tttttaatga taataaagaa ttaggagtac ttgatacaac aaagaatgat gcttcaaaag
     1381 aaaattctga tgaaaatcag tcaaaaaata cagaaatgaa tgatgaggaa agacaaaagc
     1441 agattacaga tataaaggaa aactatagat tctacgttgt aatgaatttt gataaagatg
     1501 gaaaagtaaa tattgtggat tcttatggag taaatcaaaa agttattaat caaatgttgt
     1561 tatctaaagc aagagaagat ttaatggata caagtttagg aggaaattca atttcatata
     1621 agttatctcc aataaaaaat ataacattag tatatgctgt acctaaagta ataagtaaag
     1681 gtgatagtgt agagtatggt tatggaaata cttattttga tgatggtata atttcatata
     1741 taagcaatgt agaaaataat tcttattata agatttctgg aatgatttta aaaattatat
     1801 tagctatagt tgttattgta gctttagcat tcccatatag aaaatctaaa gagttgctag
     1861 gatttgatac attattaaga ataccgttag agatactctt tattgctctt gctattatac
     1921 aggggcttgt agatttatat cctactgtta taataagtaa taccttgagt gggaattatg
     1981 tagagtacct tataaacaat gacataagtg ctaaaatggc tagcatattg gtaagtttaa
     2041 tgaatttaac ttattggttt gtaatattct caataatatt tgtatttata atacttttaa
     2101 aacatatttt aaatgttgga ataagagagt attttacaaa acatactgtg acaggaatgt
     2161 tttttgtatg gttaaaaaaa gcaggaaaaa cagttattaa tcaaattaga acaataaatc
     2221 taaaagaaaa gccaaataaa actatagtta caattttggg aataaattta ttgattatag
     2281 ttataatgtg tagtatgtgg ttctttggaa taatactcgc attaatatat tcagtagtat
     2341 tatttaagat actgtcagat tactctaaaa agacaatata tgaatataat caacttttag
     2401 atgttacaaa gaaaatttca gatggaaact tagatgtaaa tatggaaaaa gatttaggat
     2461 tttttaatcc tataaaagac gaattaggaa atattcaaac tggatttaaa aaagctgttg
     2521 acgaagaggt taagagtcaa aagatgaaaa cagagcttat atcaaatgta tcacatgact
     2581 taaaaacacc tctaacatct ataatcactt atatagattt attaaaagat gaaagtataa
     2641 cagatgaaaa tcgtaagttg tatattgata cattagacag aaagtcacaa agattgcaac
     2701 atctaataga agatttgttt gaagtaagca aggcaaatag tggagatgtt tatttgaata
     2761 tagtaaatgt ggatattgta tctctaatga agcagacact tttagaatta gatgacaaac
     2821 ttgcagattc atctctaata gtaaagaata acttttctaa tgaaaaaata atattaccat
     2881 tagatagtca gcgtacattt agagtatttg aaaatcttat aataaatata agcaaatatg
     2941 caatgccaaa ttcaagagtg tatattgata ttttagaaac agataataaa gtaaatatta
     3001 tgcttaaaaa tatgtctgca tctgagattg attttagtgt agatgatata atggagagat
     3061 ttgtaagagg agataaatca cgtaacacag aaggttctgg tcttggttta gcaatagcaa
     3121 aaagttttat agaattacag ggtggtaaga tgaatataag tgtggatggt gatttattta
     3181 aagttacaat tacttttaac aaaaattaga ttagacttct ttaataatta taaagtctat
     3241 agaataaaag agtctggaaa tatttatgtg tattctttaa ataaaaattt ataggtattg
     3301 ttttaaaata atttgtaagt attatttcaa aacagcttat aaatgttatt ttaaaacaga
     3361 atgttgattt atttaaaaaa ggtgaatcct aatatacaag gttcaccttt ttattttaca
     3421 aaatgttatt tagtgttaca gagcttataa atagcataat atctgggttt actattaaca
     3481 tagtactagc aatttatttt gaaatcttgt ataagctatt ttatattatt taattactat
     3541 tgtttgtact ttacaatgtg ataaagaatt tacctctaca gttttagtat ttactttaag
     3601 cttgctatta ttatcataga tttcgtcgag cattgcacta taagcatcat tgacattttt
     3661 attgatgtta agacttccct tagcatcctc atttttcata ccattgaaga ttctaactac
     3721 aattccatct cctttttctt ctttctttat tgcacttact gtgaaatttg aattatttac
     3781 attcatcaaa ctaaattcat tttcatatat ttgtttttcg tcattgaatg caaatagaag
     3841 tctaccattt aaaaaatcag catattcata tatttgtata ggtgttaaga atctcttagc
     3901 tgtattagct acatttgctt catcaaattt attgttatat atgtaaagac caaagtcaaa
     3961 gtttaactct ttaagtaatt gtgcatctgg tgttgctatt attttttctc cagaagctct
     4021 tcctggtcta taaagtaaat tttctcttcc catatatcca aatgacctga atagtgttaa
     4081 tgctattgta tctaaattat caccaactat ttgatattct cttacacaac cagttattaa
     4141 tgctattcct ctttgtccat cctctaaagt aacaaaactt tgcattggtt cgatagttat
     4201 aggtttttct tgccatgagt aattgtctct tttccataat gcaaggtctt tttctaatac
     4261 tgttggtctt tgtattattc caaattgttg atctgctata gaaaacttag atgctatttc
     4321 tgtattaaat aaaactctta ctctatgaga tagtgcttgg ttatttttta tattaacatt
     4381 aaacttaatt atttgttctt taggttctaa ggtaattttc attgttactg gcattttcac
     4441 agaaacttta cctatagacc tttcttccag tgaagctggt atggtcaatg aatatttaag
     4501 tatcatttct tcttgaatat ttgattttag cacttctact gaagatacag aatctaaaga
     4561 tgaaacataa atatcttttc ttggaggaga atagttgtat gaatctccat catctccatt
     4621 gtcttcaatt acagcttggt ttttataagt cttactagat tctttatcta atatttctaa
     4681 tgaaccattt ttattggctg ttattttgta atatttgttt tctatttctt tgcaattact
     4741 tgtttctatt tcacatttat ttttagtatc caattcaaaa actagttgag tatatcccat
     4801 tggtggtata tcttttactg aaagtgcaat ttttgctcta tatacagttt tgggtatata
     4861 tattttttta cttgggttta atcttattgt ttgagttaat atataatttg taatatcttc
     4921 tttttctttt attatataat taagaatttc tccattttta ttctttatca caaaatcttg
     4981 acctggaata taagtgtcaa attctacaac ttcatttctg acttctggga atccattgaa
     5041 tactgttaat gttatttctt cattattatt tttgatttga gtacttataa gtctcatatg
     5101 caaatctaat aaatttatac tcaaatctct ggcttgtttg tatcttgtgt atacatcctc
     5161 atttgctgtg tcactattac aagaacctat gctgtcatga gctgcatttt caaacataag
     5221 tttccatata tcttttataa ctagatgagg gtattcattt ccaagtgaat aacttaatgt
     5281 taataatggt tcaagtacat ttgtgatata attttctaat ttgttattca ttattttaag
     5341 gtctgacctt gaggagaata tggatttgtg tattctcata tgtttagcat ttaaaaattc
     5401 acctttaagc tctgtaaatt catttctttc attttttact gaatctatat agttttctat
     5461 attacttatt tcgtattcat tttcattatc tatttcatta gcttttttta atatatcagg
     5521 aaggtttttt cttattggtg cttggtcaaa tccatttgga aaatatacgt tattagtaga
     5581 agctttaacc ttaagttttc caattatatc ttttaaatat tcttttaaat ctgaatcatc
     5641 ttctggtata ttacctccat agtaataacc aaatggtatt tgaactgcta atacttttga
     5701 accatcagaa cctttccata taaactctgt tgtatctacc atatcatcag aaactccacg
     5761 ccaaaataat gaactatcta ttccaaaacc tctgtatatt tgtggcattt gagctgattg
     5821 cccaaaagaa tctggtacat atccaaccct cataggttca ccaaattcat tgcatctatc
     5881 tattccatag taaaggtttc taattataga ttctcctgat ataaccaatt ggtctgtttg
     5941 agtataccaa ggtccaataa ctaatcttct tctttgaact aaatctctta ttctatattc
     6001 atcctgaggt ctccatttta aataatcatc taagagagaa gcttgtgcat ccatagtaaa
     6061 gtatttaaag tcctcttttt cttccaatat gtctaatata tcttgaaaat ctttcattaa
     6121 atatattttt gaccttgatg ttgtaaaata ccattctcta tcccaatgac tgtgtggaac
     6181 tacatgtact tttgtctttt tcataaaaat cctcccaaaa tttaattgag aattgagagt
     6241 acactcaatt ctcaattatc taaaattaag tttttaattt acattatatc taactctatt
     6301 tcatcaaatt ctgtattttc cgtagtttca tcttcattga agtctactaa agttgttgat
     6361 aatattgcta ttactaaagc tccaaatatt gcacctacta agtaagccca tccagaacct
     6421 attgatataa atccatataa tccaccaact ggaggcatta tagcatgtac acctaacaaa
     6481 gctgataatc ctacacccaa agcgcatcct atcatattaa caggaactaa ttttaatgga
     6541 tttgttaaag tgaaaggaat tgcaccttct gttattccaa ttatacccat aactatattg
     6601 ccttttcctg tttcagctaa tgcattggaa aattttgatt ttcttatgaa tgtagctaaa
     6661 ccatatccta gtggaggtat aactattgcg cagtttacca atatcgctgg tagatagatt
     6721 ccctctaaca ataatgcatt tcctgccatc caagctgctt tatttactgg tccacctaaa
     6781 tcgaatccta tcattgcgcc taaaattaaa gccaatacca ccgaatttgt tccattttga
     6841 cacatttcag ctacccaagc atttaatgaa gtatttatgt ttcctaaagg tcctgctaat
     6901 acaacactca ttaaaaaacc agttaatcca actgttataa gtggcattat tataagtggc
     6961 atcattcctg atgctgaccc agatatttta atttttttct ttaaaaactt acaaacatac
     7021 ccagcaaaga aacctgctat tactgctcct aagaatcctg cattagtatt acttgctatg
     7081 agcccaccaa taaaacctgc acccatagct ggtttctcac ctattgaaaa tgctataaat
     7141 cctgctaata ccgtatttaa tagacctatg ccagtccaac caacttgttc cattaagtaa
     7201 agaatatgat aaaaaccaga tacattttta taagcatcta aacttgttat acccattgct
     7261 aaagctgtaa gctttgggat agctacaact aaagatgagc ctattacaag tggcaacata
     7321 tatgagatac ctgtcattaa gtgtccctta agttcactta aaagagattt atttttttta
     7381 ctcattttta atcctcctat ataagtttgt tatatttttt gataatatgt tataattaca
     7441 ttagacaatt ttttaatttt ttatttttct cattctatat tagagtgaga tttttttatt
     7501 aagaaatagc ttctatacac tttgttaaga cagcttctgg tttttttaac gctataccta
     7561 ttttgacttt tatcactttt ttaccatcaa atctttccat tccagatata gtttggtcag
     7621 atgctataag tataaagtcc gcatcttgtg cttcttcttt tgttatagta tttactcgtc
     7681 ccatagcacc ttgttgttct actttgattt catgacccat ttttgatgca gcttgttcta
     7741 aagcttttgc tgccataggt gtgtgtgcta atcctgctat acatgatgtt attgctacta
     7801 ttttcataat tattcctcct tcattttatt agttatatat tttactaaat gacttgcatc
     7861 actagcattt ttaacttcac ttgtaaaatc atcctccatt aaacatgtgg ctaatttaga
     7921 tagagttttt aaatgtatat ttccttcatt tttatgtggg gctaacaaac taaatacata
     7981 tctaacctta ctatcatcta aagttttcca ctctaactcg ttagttgttt taatatagag
     8041 tatggaagct tcctttacat ttacactttt tgtatgaggt atcgcaaatc catcttttat
     8101 tcctgttgga aattcatttt ctctttttag aaggtctttt aaaagagcct ctttattagt
     8161 tgcaatacct agtatataag cactatctgc aataaaattt aatacttctt ctttgctttt
     8221 taagtcttta tctataaata tatatttttc ttgtagtacc atatattttt ccactccatt
     8281 cgtaatgtct taacttatat ttatattgta atcattatcc taattaaatt aaatataact
     8341 aatttccact tcgacatgga aatttatatt atgataataa ctaatttcca tatagaagtg
     8401 gaaagaaatt attataatac aattttatta gagtaattgt attatatgag tgtaaatagt
     8461 ataatttaga agaaattcta taagggggga tatttatgca caagagattg ctcacgttgt
     8521 ttaaattatt aaatgaaagt gatgataaaa ttacttgtaa aactttaagt aatcatttaa
     8581 aagtgagtga gagaactata agaaatgata ttacttctat aaatggaact ttagagaaaa
     8641 atggtgctat tattaaaata aaaaaaggag aaggatatta tatagatatt cttaatttag
     8701 cattatacca acagtatcta gcattaatat ctgatgatat tatggattca agtgaaatac
     8761 cagattctcc tatagaaaga aatcaatata ttttaaaata tattctctat aacaatacat
     8821 atataaaact agaagattta gctaatagct tgtatgtgag taaatttaca atactaaatg
     8881 acataaaaag aataaagcct attttgtcaa agtataattt aatattagtt tctaagccat
     8941 attatggagt taaagttgaa gggaaggaaa tagatattcg aagatgtatt tctaataata
     9001 tgataaatag gaattttgaa aactatataa ttggaattac agatagagaa atagaattat
     9061 ttaataatgt agatttaata gagcttcaaa gggtagtgtt aagtgaaatt aataaattta
     9121 atataaattt tctagatttt aatctaaaaa actttattat ccatttagct attactataa
     9181 gtagaatact tgatggttac tgcttagata atgttttaga tgttgtttta acagattttc
     9241 aatcgaatac aactgttgaa aatatattta attatattga gagtaaatat actataatta
     9301 tttcaaaggc agacagagtt tatctatata atcattttat aactaaaagt tctcttttgg
     9361 ataacgtttc caatagggtg gatactaaaa ttatagagta tgttgaagaa attttagaag
     9421 taatcaacaa tcaatatact tttgacctta gaaatgatag tgtattattt gatgatttag
     9481 tactgcactt taaatcaatt ctcaattcta aatcttataa tttaaataag gtgaatcccc
     9541 ttataaatac aataaagagt aattatcctt tagcatttga aataacttta aatgctatag
     9601 aaaaagtttt taaaaatagt atttactctt taacagaaga tgaaattgga tatgtatccc
     9661 tgcatatagg tgctggaata gagagatttt ttcaaaataa tattaagtgt aaaaacgtag
     9721 tattagtatg tggttcaggg tatggttcaa gtaggttatt agaggttcaa ttaaacaaag
     9781 tttttcatga taaaataaat atacttcaat gtctatcatt taatcaattt ttagctagtg
     9841 aattgtcaga tgtagatata ataatatcga caattccttt aaatcatgat agtattccta
     9901 ttgtattagt ggatttaaaa ctattaaaaa aagatataga gaatatatct aagtcaatca
     9961 ctaataattc ccatatttat agtaatcttt tggacaattt ctttgataaa aatttattta
    10021 ttgttaatcc taaaatcaaa gataaagatg agcttataaa gttgatgtgc aacaaattaa
    10081 ctcaaagtga aattgtattt ccatcatttg ctgaatctgt attttacaga gaaagtttat
    10141 cttctaccaa tatagatgat tttttagcta tacctcatcc tatggaatta agctctataa
    10201 gaactaagat ttgtatttcc atattgaatg aaccaatata ttggagtgaa gattctactg
    10261 ttaaattaat catgatgtta gctataaata aagatgatta tataaaaata aactccattt
    10321 atgatatcct tttaaagata attcatgaca atgatattag agatagtata tctaattgta
    10381 atgattttga taattttctt tccataatca agtctatagt atagattttt atattacata
    10441 agttttaaaa actttaataa aatatttttt gtttttagca taaatagtat aagtaagttt
    10501 tgtataattt gttattattt tgtattgatt ataattatta tacaataaag attctgtctc
    10561 aaaatagaga gagaaacttg tttttcattg ataaaatgat aaaaatatat ttgatttcat
    10621 acaaaaaaga gtgtctcatg aactaaaaag ttcattttga gacaccacct ttattatttt
    10681 atattattta tctcttgtat atattactat agcatccctt aaaaacttag ctaatccagg
    10741 ttgttctcta tcataatatg atgtaaaacg ttcatcatat acatacatct gtgctagaga
    10801 ggaatgagct tctttagagt atgtgttcca agtgtaactc aaccatttat gatgtaattt
    10861 tgcaacttcc tgagccagtt cactagaggc atcaccagtt ttaaaagctt ttttaagttt
    10921 actaattatt tcgatattta ggttttgcca attttcataa tcttgcttag acatattttt
    10981 aagcttttta tttgatgtat ctataatatc tttaccatat ttctctctaa tttcagttcc
    11041 ataatttttt tcattttcat caatcatctt ttctttaaaa ccttcaaatt tttctatatc
    11101 tgtcatagtg tattttccct ccttcagagc tatagtttta gttacatttt ctattagtaa
    11161 atcaatttgc tttcttttgg ctagtaattt attatggtgt tcttttaacg cacttaaact
    11221 atcgaagttt ggtgaattta taatgttttt tatattttct aagttaattc ctaattctct
    11281 ataaaaaagt atttgttgaa gtttattaac ttcattttga ccatatatac gataacctga
    11341 agaatttatt ttaagtggct taagaagacc tatttcatca taatatctaa gtgttctagt
    11401 acttatacca gctagtttac ttaatttttg aactgtatat tccataagta atacctccta
    11461 taaattaaat atacactttt acgcaacgta aatgtcaata tattaaaata taaaaattta
    11521 tatcttttaa taaaaatata aaaaaatatc tttactggat attttacaca caactgtcaa
    11581 tactcactaa tgaaaattaa aaaataaaat ttatagacta tctttactaa aatatagaag
    11641 gtctatagcc taggaggaac tgtatgaatt taaaaagaag caatgaaaaa cgagaagagt
    11701 acagaagaat gtattcagat aaagaaagtt ttttaagttt gattcaaaat tttacaagtg
    11761 tctcaatagc aaaagaatta actcttaaaa atatagagtt agaaacatct tttatatgtg
    11821 aatataaagg aaaagaagtt gatataattt ataaggtatt ttctaaaagt ggcaaagtat
    11881 cacattatat agtgttagaa tttcaaacag aaatggatac tgaaattgtc ccaagattga
    11941 aatcgtatag agaacaaatt tggaaaagtt ttattatgaa aaaaagcctt gaagaaatag
    12001 aagataaaaa ttttaaactt cctaaagtta taccagtggt tttatatagt ggacctgaaa
    12061 ggttaagtag aaaaaaagga attttggata ttgtagaatc tatatcggag ttcagtaata
    12121 attctagtat gaaaaattaa aattacataa tgtgtttatt ggtctaaaat ggaaaactgt
    12181 cgcataaact tgaatatgaa aatatttata gatacaggtt ctatgtggag gtgttccatg
    12241 agaaataaca agtctaaggt attgctagga atactttgtc tagcaacagg aatggctgta
    12301 attctgtcaa tgttgcttcc tggctggatt tggtcagcac ttaccgcatt aatacttatt
    12361 ggatgtggag cattattatt tttttgttaa aatagtttta actataaagt gtggggtgag
    12421 tattgatgaa agtagtaaca ataaaattac caaaatggat ttcaagtata atcctaaaat
    12481 ttatgagaaa gagtaaaaat gattaaatta aaaagcctac ttacggaagt aggcttttaa
    12541 acgtggttac taagctcttt caattttacc agaacgtaaa catctagtac aaacttttac
    12601 tctcttagga gctccatcta taatcgctct tacgcttctt aagttagctg accatgttct
    12661 tttattatgt ttatttgagt gtgatacttg gtttccagaa accttacctt tatcacatac
    12721 gctacatact tttgccatct acgcacacct ccttaaaatg ataactattt aaagactaaa
    12781 aacatattta tattatcata aataacaaaa atttgcaata gaaatatata tttgccttac
    12841 acatattgaa gaatttctta ttatcatata aaattatata tataatttta tataaagtaa
    12901 atatatttgg aaaataatta ttatttttct ttgaatatat atactataga agaaatttta
    12961 tccaaaaact tttctaggtt taaattatac catatttata gtatgatatg attataattt
    13021 tatataataa ataagtaaaa atagtaaaaa ataaattaag aaatgttttt aataaaaata
    13081 agcttaaggg ggtcaaccat gagtgcaaag gtaacgaatc aatatggaaa tatagaaata
    13141 gataatcaag taatagctca agtcgtatat agagctgcaa tggaaagtta tggcttagta
    13201 ggtttagcat ataagtctaa aggaatagta gagctattaa aaggcgagaa cgcaactaaa
    13261 ggtgtaagag ttgaggaagt agaagaagat gccatagcaa tcgaacttta tgttataata
    13321 caatatggta caaatatatc tactgttgcc aataatataa tagatagagt aaagtatgta
    13381 gttgaaaaaa tgactggcgt aagagttact agaattgata ttaatgtaca aggaataaga
    13441 gttaagtaat ttcataggag gaaacaaatg attcaatata tagatggtaa aaggttaagg
    13501 gagatgttta tttcaggtgc aaacaatctc caaaacaata aagagttagt tgacaagtta
    13561 aatgtattcc ctgtaccaga tggagataca ggaactaata tgtcattaac aatatcttat
    13621 gccttaaaag aattagcaaa agttgaaaat gataatatat cagatatcgg aaaagcatta
    13681 tctaaaggtt ctttaatggg agctagagga aactcaggag ttatactttc acaaataatc
    13741 agaggtatag ctaaatctat agaaggaaaa agtaaattat caacagaaga tttagcaaaa
    13801 gcttttaaaa atggttcaga cacagcatat aaagctgtaa taaaaccaat tgaaggaact
    13861 atacttactg tagttagaga aagtggagag tttgcaataa aaactgcgaa aaaagagaaa
    13921 gatgtagtaa agtttttaag tatgcttgtg aaggaatcaa attcttcatt agaaagaact
    13981 ccagaccttc taaagaatct aaaagaagct ggtgtagttg actctggtgg aaagggtctt
    14041 gtacttatat atgaaggaat gttagcttct ataaaaggcg ataacataga aataaaaaat
    14101 gttgacctag atactaatat ttcaacaagt atggattttg caaagagtac tactagcaca
    14161 gatgatataa aatattgcta ttgtacagaa tttattttag aaagttctaa ggtagaagat
    14221 actaaaataa gagacataat gatggcttat ggtgatagtc tagcagtagt tggagatgat
    14281 ggagtcataa aggtacatgt acacactaat gacccaggaa atgtacttca agaagctcta
    14341 aagtacggtc aattattaac tataaaaata gaaaatatga agttacaaca tgaaaataca
    14401 ttactggatg tagaagaaaa gaaagaaaat gatagtgaac ctttagaaga agagaaagag
    14461 tttgggttta tagctacttc tatgggtgaa ggtttggcaa atatatttaa agatttcgga
    14521 gttgaccata taatagaagg tggtcaaact atgaatccaa gtactgaaga ttttatgaat
    14581 gctataaagc atataaatgc taaaaatata tttattttcc caaataacag caatataata
    14641 atggctgcta atcaggcaaa agaattgagt gataaaaata taatagttat acctacaaaa
    14701 aatacgcctc aaggatttgc tgcattagta acttttaatg gagaattaag cgaagatgaa
    14761 aataaagaag ctatgatgaa tgctttaaac tcagttaaat caggtcaagt aacttttgct
    14821 gttagggata cagtgatgaa tgaaattgat gttaaagaag gtaatataat tggaattgca
    14881 gaaggaaatt tattatcagc aggagattat gtggatgaag taacatcaaa ccttattgaa
    14941 aagttggttg atgaagatac tgctataata acattattct ttggtgaaga tgttacagaa
    15001 tctcaagcaa atgaactacg tacttcttta gaagaaaagt ttgaagatgt agatgtagag
    15061 ttatattatg gaggacaacc tctttattat tacttaattt cagttgaata atgattataa
    15121 gaacctgtat gaccaagtag cttttataaa attggttgta caggattttt gtgtagtagg
    15181 tgatatattt gttggattta tataaagatg ttcaatatgt taagggaata ggacctaaaa
    15241 aagcagataa attaaataaa ctgggtatat ttacactaaa agacctattg tattattttc
    15301 caagacaatt tgaagataga aataatctaa agaaaatagc acaattagaa gatggtgaga
    15361 aagttacgat aaaagcagta atatcaagta taaatacatt tagcccaaaa gaaggtatga
    15421 ctttaacaaa gattgatgta aaagatgaga caggctcggc taaattagtg ttttttaata
    15481 aaagttatat aaaaaatact tttcgtccag gagattcaat attagttttt gggaaggtta
    15541 agaaaaaatt taataactta gaacttacat catgtgaatt agaatatctt actaattcac
    15601 ctaagaatac atgtagattt atgcctgtgt accagcttac atacggtgtt acaaataaag
    15661 aaattatgag tataattaga acagtacttg aagataaaga attaattata caagaatata
    15721 tgccacaaag aattatagaa aaatataggc tatgtagtat agattttgca gttagaaaca
    15781 tacattcacc aagtagtaag gaatcattaa aaatagcttt atatagaatt gtatttgaag
    15841 aattacttat actacaacta gggttgtttg tatttaaaag tggcagaaat aaagaagatg
    15901 gaataaagtt tgaaacaagt aaagacttaa aaaaaataat aagtgcttta ccatttaaac
    15961 tgactaaagc tcaaaacagg gcattagatg aaattataca agatatgaat ttagagaaaa
    16021 taatgaatag attggtacaa ggtgatgtag ggagtggtaa aacagtagta gctctcttag
    16081 cacttgcaaa ttgtgtttta aatggttatc agggcgcttt gatggcacct acagagatat
    16141 tagcagggca acattacatt tcacttactg agtctctaaa agattttgga ataaatgtag
    16201 gtctattaat aggtagtctt acaaaaaaac aaaaggatac agtattggaa caaataagga
    16261 ataatgagat tgatatatta ataggtacac atgcactaat tgaagataag gttgaattta
    16321 ataatattgg tttagttata acagatgaac agcatagatt tggtgttatg caaaggagca
    16381 aactttcatt gaaaggagca aatcctgata tattagtaat gacagcaaca ccaataccaa
    16441 gaactttagc acttatactc tatggagatt tagatatttc tattatagat gaacttcctc
    16501 caggaagaca gccaatagaa acaattgcta ttgaaaaaag taagcgagat agagcatata
    16561 ataaccttgt aagaagagag gttgaatcag gaagacaagt gtacattgta tgtcctttgg
    16621 tagaagaaag tgaagctata gaagctaaat cagcagttga acttgtagag gaattaagag
    16681 ctgaatattt ccacgattta aggcttggat tgcttcatgg taaaatgaaa tcgagtgaaa
    16741 aagacgaagt aatggaacgt tttaaaaata aagaaataga tattttagtt tcaacaacag
    16801 ttatagaagt tggtgtcaat gtcccaaatg caacattaat gataatagaa aatgcagaaa
    16861 gatttggact tgcacaatta catcagctta gagggcgagt tggaagaggt agtcataaat
    16921 cgtattgtgt actaatttat gattctaaaa cagatgtatg tagacaaaga atggctataa
    16981 tggaagagac caatgatggt tttaagattt cagagaagga tttagaaata agaggacctg
    17041 gagaattttt tgggacaagg caacatggtc ttccagaact taaagtagca aacttattta
    17101 aacatataaa aatattgaag ttagcacaac aagaagctcg atatatattg ggtgaagata
    17161 ataatctaca attgaaagaa aacatggcac tcaaaaaaga aattatagat aaatttaaag
    17221 atacattgaa agaaatttca ttaaattaat tttaaattat gttaaaatat tactaatgta
    17281 ataatgtgct gaagggagat tgtcatattg agagtaattt caggaaaagc gagaggatta
    17341 aaactaaata ctccaaaaaa tgaagatgta aggccaacta cagatagagt aaaagagtct
    17401 ctatttaata tgataaattc atatataatg gaaagtgaag ttctggattt atttgctggg
    17461 actggttctt taggaataga gtgcttatct aggggtgcca agtcatgtac ttttgtagat
    17521 atcagtaagg aaagcataga tattgttaag tccaatgtaa aaaaggcaag agttgaaagt
    17581 gaaagtatta ttttaaacct tgattttaag actgcaatag ataaacttaa attacaaaat
    17641 agtaaatttg acataatttt tatggaccct ccgtattata aaaatatgtt tatagaagct
    17701 attgaaaaaa ttgataattc taatctattg aacgaagatg gaataatagt tgtagaacat
    17761 gatacgaatg atttatttcc agataagata tgtaaattgg agaaaactag agataagaaa
    17821 tatggaaata ctacattaac tttttataag atggaggcat aatatggaaa ataaacctag
    17881 aaaagctata tttgcaggaa gttttgaccc aattactaat ggacatttag atattatttg
    17941 cagggcttcg aagttatttg acgaattaca aataggagta ttaaataatc ctaataaaaa
    18001 gggtctattt agctttgatg aaagagtaaa acttatagaa aaaagtacaa gccatttaaa
    18061 taatataaaa gttgtgactt ttgatggttt attaataaat tactgtcagg aaaatgggat
    18121 tggagcatta gttagaggtg tgagaagtgg tgctgatgtt gattatgaac ttcagatggc
    18181 acatatgaat agagagctta atccagatat agagactata attttaccaa gttgtacaaa
    18241 atattcattt ataagttcat ctttaataaa agaggttttg ctctttgatg ctgatataaa
    18301 aaatttggtt ccaaagattg ttttggaaga attaaagaaa aaaactagtg ggggtaattg
    18361 atatatgaag atagatttag aaatgattga actatttgag cagttagagg agatatttag
    18421 aagtgcttct acaatccctt tctcacataa atgcacagtg gataaagatg aggttttagc
    18481 aataattaat gatataagaa ctttaatacc agaagaagta acacaggcag tatggattaa
    18541 taaagaaaga aataaaataa taagtcaagc taagcaagat gctacaaata tagttgaaca
    18601 agcgaaaaaa gaagctgaga gaatacaaca agaatatcaa gataatatag aggaattaaa
    18661 gaaaaattct gaagatgtag taaaggcata tgtagaatcc agcgaacctg ttgttcaagc
    18721 agaccaaaga gcaaaagata tagttgatag agctgagaga atagccaatg agataagaat
    18781 tgggtcaatt gaatatgcag aggatgtgct ttctagtgtt gagtataatt taagagaaat
    18841 attagaagaa gtaaaaagag ataaagctga acttagacct aaaaaataaa ttttaaataa
    18901 ataaaaatgg agctatcaca caaaaaattc caattgatgt gtgatagctc tcttttaata
    18961 tagcaatttt ttgttatata attgtgcttt ttagattact tatatcttat tttgatatta
    19021 taatgtagct taatctttta tttgttgaaa tcaacttatt tatttgtata aataatatag
    19081 gttattttca aattttttta ataattttac cactgtcttc ttttagatta gggttagact
    19141 gtgcaaaatt ataaaataat actaaatcat gtgcaattat ttggtatggc actacatgta
    19201 gaaaatatag ataataaaat taaggaggta gtatgaagga aagggctaaa aaaatattag
    19261 attttattat taaaaataac aatgtaacat cacaagaact acaagaaagg tttaatgttt
    19321 caaagagaac tatttattac gatattctag ctataaatga acaactagga aaaagtggta
    19381 atataaaaaa tgtaaaacac aaatttatat ttgaaggtaa tttatgtgat gcaaggaaaa
    19441 taatttcaac tgaagaagat aaatttttag atagtgatta cagaaaaact tttatattaa
    19501 acaagatatt acttggcgaa aaaatatcaa tagaaaaact aacaaatgaa atgttacttt
    19561 caaaaaacac agtagtacaa acaataactg atgtaaaaaa ataccttcaa acaatggggt
    19621 taagattaga gtataaggga aagtacaaaa ttataggtga tgaatacgtt attagagagc
    19681 tgtttctgat aattgttcaa gaaaatgtac tggaaattaa ttctataagt aaggaagtaa
    19741 gtagtttcga tacaaaagga catataaaac ttactgatta ttctttatta aatctaacta
    19801 agtttgtgga atttctaaat aaaaggataa gagatgggaa aacattatac tcatataaat
    19861 atctaaatga agctaaaaaa attagttact ttagtaattg taaagaactt ttatgtgaag
    19921 aagccaatga aaatgaacaa gcctatatat gcacttacat aagttcactt cctagcttaa
    19981 atagtgaagt taaggaagat gttgttgaag aatatgtaga taaacttata gataagtttg
    20041 aagtaaatac agcaataaaa ttagaaagta agcatgagtt taagaaaaat attttaaggc
    20101 acctccactc ttcttacaac agaatacgat ttaagtttcc tattagaaat ccaatgttag
    20161 atgagactaa gtataaacat gagtcacttt ataaaattat aaaatcaatt atagaaaatg
    20221 aagaagagtt tcctgtattt gaggggataa gagaagaaga gataggattt atagcagcgt
    20281 attttggagg gtatttaaga gggtctaggg ataatggcct aagaagaaat aaagtcttac
    20341 tagtttgtcc taatggtctt atggtatcaa agagtttgga aatacaacta tataaataca
    20401 taccaaccat agaaatagta ggtatagtgt ccattaagca actaaaagaa gtaaatgtat
    20461 actacgatta cataattaca actatagata tacaaaatgt aaacaatgta attgttgtaa
    20521 atcctctttt aacatcatca gatgttcaat tacttatgaa caaactaata agtgtaaaag
    20581 aaaacgagaa gtatttcaat cttgaattaa taatacaagc tataagaaaa aatggagtta
    20641 tcaataatga agaagcacta aaagctgatt tattaaacat aatacacaag atagatgaag
    20701 gagagatgta tcaaccaatg ttaaaggaat taattaatgc agaaagggtc aatataataa
    20761 aaagtgttag agattggaaa gaagcaataa aaatagcctc aaagccattg ttagaagata
    20821 actctataga agagttatat atagaaaata tgataaagtc agttgaaaaa tatggaccat
    20881 atattgtttt agctgataga tttgctctac cacatgcaag ttctaaagaa ggagtaaaca
    20941 agcttgctat gtctttacta atagtagaag atgaagttga tttattagga aaaccagtaa
    21001 atatatttat ggtattagct gctgtagata acactactca cattagagca ttagcatcac
    21061 tatcagagat gatgtacgag gaagaaaatg taaaactaat aattaatggt gataaaagtt
    21121 caatactaga acttataaat aagcaaaatt aggaggaatg aaaaatgaag atattagcag
    21181 tatgtggttt tggtgtaggt tcatcaatgg tattaaaaat gacaatacaa aaggctctaa
    21241 agcagttagg tatagaatgt gaagtagaaa atacagatat aaattcagct agaggtgctg
    21301 attgtgactg tatattcaca agtcaagaat tgttaaatga attaaaagga acttgtaatg
    21361 tagatgtata tccagttaaa aagtacatgg atttagcaga agtcacagaa gttgttcaaa
    21421 cttttttaag tgataaaaat gataagaaat agtataagtt ttaaaaagta aatttaagtt
    21481 aatcagaata aaataaaaac ttactaaatt caaaaatagt aagaaagaga ggagggattt
    21541 agtggtagaa aacttaaaca gttgacctat cactaaaaaa tataatgctt gattttattt
    21601 caaataatat tttacgtaat ccatctatgt taattggatt aatagcagca tttggtcttg
    21661 tactacagaa aaagaaagca tctgatgtta taaaggggtc actacttgca gctttcggaa
    21721 ttataattat ggagactgga acaggtatgt tagttagttc aatagcacca attaatggag
    21781 ctttccaaag tataagtggt ggtgcagtaa caaaaggttt aagtgatgtt acttttactg
    21841 caagttatgg tggaattata ggtttagcaa tgtttgcggg tttagctatt cacctaatga
    21901 tagcaagatt tactcctatt aagacaatct tcttaacagg tcacatgctt tattggttcc
    21961 cattcatatt tgtagcttca ggagttgaag ctggattaaa tggtggagta ttaatagctt
    22021 ttgcagcagt attatcagca ctctattggt caataatgcc ttggttatta agaaaatatg
    22081 tgtttgatgt tataggagat gattctttta cattaggtca tccatctggt tgtttagcac
    22141 ttatatcagg atttgttgct aaattagtag gaaataaaga aaaatctaca gaagatataa
    22201 acatacctca aagtttatca ttttttagag aaatatctat aagtggtgga atggcagtgt
    22261 ttttaatgtt tatagttgtt gggatagtaa taccaggtgc gtttaaagat agtaatgagt
    22321 ttattggaac gtatgcaata aagcaaggtc tttcttttgg agcaggtctt ataataatgc
    22381 ttcatggggt aagaatgcta ataaatcaaa taatgccagc atttcaaggt atatctgaaa
    22441 aagttgtgcc aaatgcacta cctgcttttg actgtccaat attatttaat tataaaccaa
    22501 atgctgtatt aataggattc ttagttgcta tgctaacatc tacagctatg attttaatat
    22561 gtaactactt taatgtattt aatttgctac ttataccact tgttataact tcattctttg
    22621 agtgtggagc agcagcagta ataggagaat cacaaggtgg tatacgtgga gctataatag
    22681 gtacagctat agctgcaata tttatggtag ttttagtagg aatatctgct gtaatttact
    22741 caggaactat tcaaaattgg atactaatat ttggaggtaa cgacctttca ttatttggta
    22801 taattagtaa gtttataggt tcactatttg gaggaatatt atagatatga aatttaaata
    22861 catagatgag ataagaagat taattgatat aatagaaaac gaagaaaaag tcaatatgga
    22921 aaaagcagta caaatccttg ttgaagcaat agaaagtaaa aatgctatat tttcatttgg
    22981 agcttcacat gcaggaatat taacagaaga attattctat agagcaggtg gctgtgttct
    23041 tataaatcca gtatttgcaa aatctctaat gttagataca gcgcctataa ctcacacaag
    23101 taagatggaa agattagaag gatatggtac agctctagct gataaaactc caattaaaaa
    23161 aggagatgta gttatagttc attcagtatc tggtagaaat ccagtaagta tagagtttgc
    23221 tatgaaagct aaggaaaata aggcaatagt aatatgtata actaatttaa gttactcaaa
    23281 aagtgtatct tcaagacatt cttcaggaaa aaaactttat gaagtatgcg atttagtgat
    23341 agataatcat ggtcaaaagg gagatgcctg tgtagagata gatggattaa accaaaaagt
    23401 tagtccaact tctactgtaa tagcaactac tgtgatgaac tctattatag ctcaagctac
    23461 tcaggaatta gttaataaag ggctaaagaa tcctccgatt ttctatagtg ccaatattga
    23521 tggtggagat gagttaaata agaagatttt tgatgaatat aaaagtgtaa ttcattatga
    23581 atattaaggt taaaaaaata tgttaatgaa ctttgaagtt tatcaacaaa gaatattaat
    23641 aatatataat aaatgctatc atttaaaagc tgtaggtaaa aatctacagc tttttattat
    23701 tgtagattaa aaagatgcgt aattaaattt taaattggag aaaataaatt ggaaggtatc
    23761 aattataaat attagaaacg ttgtcataga aaaaaactat attaaaaaaa agtacataga
    23821 aaaaactgat tagacaataa atacatgctt tgataatatt ttaaatggag tgaaaatgtg
    23881 tagaattata atgaaaaaag aaaataaaaa tgaaaaaaga aaataaaatg gagggaataa
    23941 aagtgggtat gttaaaaaaa ttatgttcac tagctatggt agcaattatg actacaacac
    24001 ttataacagg gtgttcaagt ggaaatggaa aagattcaaa aaaggatggc gaaaaagaaa
    24061 aactagttct ttggatgcca ccagtagaaa ataatatgaa ggaagtttgg gaccctattt
    24121 tagataaatt tgaggaaaaa aataactgtg aagtagattt acagataatt ccttgggata
    24181 attattctga aaaatttgca acagctataa gtgctggcga aggtccagat gtaggatata
    24241 tgtatgcaga aatgtatcca cagtttatag aaagtggagc agtagaagat ttgactaact
    24301 atgcaactaa agaggataaa gagcaatcta tttatataaa aacatctgaa atgatgggtg
    24361 gaatgtatgg tatgccattc caagcagcaa atccaggggt attatactat aacaaggata
    24421 ttttagagaa attaggagag aaacctccta agacatggga agattttaaa agaatttgta
    24481 aaaaagcaac acaagatact gatggtgatg gaaagataga ccaatgggga ttagctcagg
    24541 gatggggagc taaaactttt ggtaacttaa actggaactg gtatccttat ttatggcaag
    24601 ctggtggaga tatttttaat gatgatttaa aatcggttaa atttaatgat aagagtggtt
    24661 tagaagcagc taatttctta aaagaattac aagcatatgt gcctgaagat tctttatcta
    24721 aagatagtaa tgaaatgata gaaagcgtat ttggaccagg gaaagcagca tttacaataa
    24781 tgttatcttc tgctgcaaca agtgtatttg atgaatcatt cccggattta aattgggggt
    24841 ttgtaactgg actagaagat aagaaagcag caacttttgg agcagtagac catttatctt
    24901 taatgtcatc tgctaaaaat aaagaacttg catacaagtt aatacaacat atgttaagtg
    24961 ttgaatcaat gacagaattt cataaagcaa taccaagagc accaataaca aaaggtgaac
    25021 catatcaagg tgatgaaaga ttcaaagaaa tggtggaaaa tgacaaaaat gtatatagac
    25081 cattagtagt aggacctcat ggagttgaaa tatatgaata tttatggaaa gaacttcaaa
    25141 ctatgatttc aggagataaa actccaaaac aagcattaga tgatgctgca aaatattcaa
    25201 atgatttatt agcacaataa aagtttaaag ctgtaatatg ttgtaaagga ctttacagat
    25261 taaaattagc attgtattta taaatacaat gctaatattt atattggaga ggataaaatg
    25321 gaaaacttag ttagagagtt tgaagaaaat tcaaaaagaa aaaaaacaaa attaaatatt
    25381 aaaccatata tgtatatatt gccattggga ataattttag tggcgtttta tgtgatacca
    25441 ataataatgt caatatactt tagttttaca aagtataata ttattagtcc agccactttt
    25501 ataggtttag aaaattataa aaaattattt acagatgaaa cattaaaagt atctattata
    25561 aatactatta aatttactgt tgttgtagta ccttgccaaa ctatattatc attgattttg
    25621 gcagtttgga ttacaggaaa aggaaatagt aaaatagctt catttgctaa aggtgctata
    25681 tttataccag tattatcatc tatggttttg ataggtatgg tatggagggc tttattgaat
    25741 ggagaaggtt ctattatata tcaggtatta ggtgcctttg gaatagaatc atctaaactt
    25801 ttaggagata gtaagacagc attacctaca cttatgttta tatctatgtg gaaaaatata
    25861 gggtatttca tggtaattta tatttcagct ataatgaatt taccaaaaca ctgctatgaa
    25921 gtagcgaagg ttgatggggc tacaaaactt caagaattta taaaaataac tgtaccttta
    25981 ttgaaaccaa ctacaataat ggttgtattt ttagggtcga tatggtcatt acaagtattt
    26041 gacttaattt atactgtgac aggtggaggt ccaggtatat ctactatgag tatagttatg
    26101 catgcattta acttaaactt taaaaacttt aattctggat atgcaatgac tgtagcaaac
    26161 gtattatttt tgttaatagc agtagtttcg attttacaaa ataagcttgt aaaacgagac
    26221 aattcagatt tttagaaagg taggtataat atgaaaacag caagaagatt attaagtgat
    26281 ggtttacttt tatttatagc gtgtgcaagt ttggtacctt ttatatacat gttgataata
    26341 tcattgaaga taacatataa ctcatatagt ttggatatat ctttttcaac agtaactctt
    26401 caaaactata tagatatctt tactaaaaaa ggatttgcac aatatttttt taatacagca
    26461 atagtatctt tttcaggtgt attattaaat ttagtattta gtacattagc aggatattct
    26521 tttgcgaaga tggattttaa aggtagtgat aagttatttt tatttatgat aatgacttta
    26581 attatacctt cccaagttac aatgatacca ctatatataa taatgaaaca cttaggatgg
    26641 ataaatagtt atcttgcact tattatgcca ataccaactg catttggcgt atttataatg
    26701 agacaagcta ttttaggagt accaaaagag ttattagaat cagcaaagat agatggttgc
    26761 tcggatttta gaattttaat acaaatagta ttgcctttaa tcaaaccagc attaattaca
    26821 ctagcaatat ttacttttat gggagcatgg aacgagttta tgtggcctct tatagctaca
    26881 actaaagatg caatgagaac attaacagtt ggattatcta ctcttaagac attccagata
    26941 actaattatg gacagatgat ggcaggggct acaataacat ttttacctcc atttattttc
    27001 tatttgattt tacaaagtaa atttgttgaa ggtgtgtctt tgtctggtat aaaaggttaa
    27061 atagtctaat ttaataacat aaaagaaact tatataaata tggaggaatt taaactatgg
    27121 aaatttcata tgtactgaaa gattgcacat tcaaacacaa taaatactca gttatagaag
    27181 ctgaaacttg gaaagagaag gatttggata ttgtagtagc taaaggtgaa aaatttgcat
    27241 ttcaaattat gctaaaagca acagaagaat ttaattgtac aattgataaa agcagtacta
    27301 tatcatggaa aggactggaa aatagagtaa gattagcatt aaatgtgcct aatagtttag
    27361 aaaataattt tagtataaat atattaggat atgttcaaga tgatacaaag gcatttgtga
    27421 atgatgctat tttaagggat aaagatgtgt tagtagagca atatttgcct caaacatttt
    27481 ggattgaagg tcaagtacca gaagattttg aagaaaataa ccttaatata ggtattgata
    27541 tatttaaaag ctttggatat gaagatgaag aaaaagtttg tacaattgat gttcctgtaa
    27601 aagtaagaaa tgtcgtgcta aagcctttag ataaaagcaa gttcttttta gatttatggc
    27661 aacatccaag ttgtttagct agaatgtata aagttgagtt atggtctgat atacattttg
    27721 aaatagtcga taactattta aaagaattag catctttagg agaaaaagtt gcaacagtta
    27781 tagtttcaga ttatccatgg gcagggcaaa gttgttataa agtttataaa aatccatcaa
    27841 acctatatga atataacatg gtaagtgtat caaaaggatt ggatggaaag attaaatgta
    27901 actttgagag tatggatagg tatataagca tagctgataa gtataaaatg tctaaggaga
    27961 tagatttatt tggtctatta ggtaattggt gtgctggtga gtttggaaat cctgtagaag
    28021 gttataagga ccctataaga gtaagatatt ttgatgaaaa tgataaagta tttaaattta
    28081 taaacaatac aaatgattta aaagaatata taggattagt attaaatcat ttaatagagt
    28141 gtggattatg ggatagagta aggattatag ctgatgaacc aaataatcca gaagtagtca
    28201 aagagtgtat agaatttata aattctactg taggtactca tcaagttaaa tataaatcag
    28261 caactcacga ccaaaacttt ttagatagag ctaaagatga aattgatgat atgtcaataa
    28321 atttaaaact tactatacaa aattataaag atatagaaaa tcttaagaaa aaaatcaatg
    28381 acaagggtgg gatattgact tggtttgtat gctgttttcc agagaaacca aatagttttt
    28441 taagttctcc ttttgtagaa aatagaataa taggatggta tacttattac tttggtcttg
    28501 atggattttt aagatgggac tataatttat ggacagaaga cccttggaaa gattcaagtt
    28561 acaaattccc tatttggaaa gctggagata tgttctttgt ttatcctgga aaagatttaa
    28621 agccagttag aagtgtcaga atggagaatt taagatttgg aatacaggac tttgagttat
    28681 ttacaatgtt agaaaaagaa aagggcagag agtatataga agttgagctt atgcaagaat
    28741 tattaaataa aaaagaaaat gctgaaatta aaggttttgg tgacatagaa ttaggatatt
    28801 cacttgataa ttggggatat gataaagtta aaaaatatgt attagagttg ttagacagag
    28861 tataaaaaaa tataagagtg tctcaaaatg aactttttag ttcatgaggc actctttttg
    28921 tatgaaatca atatagtttc attgtttcaa caatgaaaaa taggattact tatattctaa
    28981 gatagccctt tagtttttta gttgtattca atgttttaaa actaactata aacgctataa
    29041 agtttataaa tgacaataat tatatgtaac atgatgtact ttaatttgct ataataggga
    29101 ttgaataata ttatatggaa gataacattg tttgggggga ttaatataaa aagaataggt
    29161 attattataa tgtttttttt aagtatagct tttattggat tttatcttgc aataagaggt
    29221 aacagtgata cagttaagat gcaacgaggg ataaatatag gtaatgcact agaatcacct
    29281 aaggattttc cgtgggatgt aaaaatgtca aataagtttt ttgatgatat aaaagatgca
    29341 ggttttgaca cagtacgtat accagtaagg ttttctgatt atacgtcaga ttctgataat
    29401 tttaaaattg atgaagaatt ttttaaaaag attgataaat atgtagatta tgcattggat
    29461 aaagacttaa tagttgtgtt ggatttacat cattttgaag aaattatgaa agaaccaaga
    29521 gttcataaag aagagttttt aaaaatatgg caacagatag ctaataggta tcaaaaatat
    29581 gataaaaaat tggtatttga attattaaat gaacctaaag aaaatttatc ttcacaatta
    29641 ctaaacgaat acattgaaga agcaataaag ataattagaa aaactaaccc taaaagaact
    29701 attatagtgg gaccatataa tttctatcaa attgattatt tgaatgaatt aaatgttcca
    29761 aaagattcta atatagtagt ttcttttcac tattacgaac caaatgattt tgcatttcaa
    29821 ggaaacattt atcataatgg atttgagcac ttgagcaata ttacttggga aggaacaaat
    29881 gaacaaatgg attatttaaa aaaaaggttt gatattgtag aaaattgggc aaataaaaat
    29941 aaggtaaaaa tattcttggg tgaatttgga gtaacaaaag aagcaccaga agcttcaaga
    30001 agagcttgga taaaagctgt aagagaagaa gctgaaaaac gaaacttttc atgggcttat
    30061 tgggagcttg cttctggatt tggtatatac aatcaaattg aagggacttg ggatagagac
    30121 atattaagtg cattaataga aaagaggtga ttttatgaag aaaaattcaa aaaagatact
    30181 tataacttta actattatta ctaatatcgt atatatattg tggagaattt tttacacagt
    30241 acctaaagaa gaagggatgt ttgccttaat ctgtgctata atacttcttt ttgtagagat
    30301 aatgggtatg atggaaatgt ttgtacatta ttatggtatg tcaaatattg agtatcctga
    30361 aaaaccagtc ataagtgaag aactttatcc acatgtagat gtattcattg caacatataa
    30421 tgagtctgtt gatttggtga gaaaaactgt aaatggatgt attcatatgc agtatccaga
    30481 taagaaaaag gtacatatat acatatgtga tgatggaaat cgtgaagaaa tgcgtattct
    30541 agcagaaaaa atgggtgtaa attacataac tagaactgaa agagaaggag caaaagctgg
    30601 gaatttaaat aatgcaatgc aacatacaaa ctctcctttg attgctacgt ttgatgcaga
    30661 tatgatacca atgcatgact ttttaattgc tacagtacca tactttttga aaaatgagca
    30721 agctaaaaaa gatggagaaa aagaggagta tgaaaaggtt ggttttgtgc aaactccaca
    30781 gagtttttat aatccagact tgttccaatt taatctgcac tctgagggaa ggattccaaa
    30841 tgagcaagat tatttttaca gagatattca attggctaga aataggacaa attctgtaat
    30901 ttatggtggt agtaatacag ttatttcaag ggaagcactt gaagaagttg atggatttta
    30961 tacttattct attacagaag attttgcaac aggtatctta attcaaagta aggggtatag
    31021 atgttatgct attccagaag tacatgcatc aggtttatct cctacagatt taaaaagtct
    31081 tattaagcaa cgtgaaagat gggcacgtgg atgtatacaa actggtagac gtctaaatat
    31141 tttgtttagg agaggtttag gtttttggca gaaaataagt tatatttctt caattactta
    31201 ttggtatgca agtataaaac gctttgtata tataatggca cctattttat tttctgtgtt
    31261 taatgtgatt gtagtaaaat gtacattact tcaagtattg gtattttggt tgccaatgta
    31321 tattttaagt agtttatctc ttaaaatatt ttctcaaaat ataagaaata ctagatggac
    31381 caatatatat gaaactataa tgtttcagtc tttaatgcca gcagtaatac ttgaaacgtt
    31441 tgcaatatct aaaaataaat tttcagtaac aaataagaat aaattagaag aaaatagaat
    31501 gtataagttt ttacagggaa taccttattt tatttacatg gtcttatcta tcataggaat
    31561 tttaaaaatg tttgtagcaa tatttaaaat gagttcaatg acatattcag ttgtattatt
    31621 ttggttgatt ggaaaccttt ttaaccttgt tatggctaca ttatttatat cagggagaca
    31681 acagttaaga aagtctgagc gttatattgc tgaaattgat tttaagttaa agcaaaactc
    31741 ttatgttctt tcttcaaaaa cgattgatat atcagaaaat gggtttgcat ttttgctaga
    31801 aaatccagaa tatatttccc cagaagaaga gttcgaggta gagtttagag aaaaatcagg
    31861 aaatgaaatg tatatagcta atatgaaagc aaagattgtt aatgttgtgg aagtaaattc
    31921 aaagtggaaa tatgcagctt atattacaca tattgaagat agtgaaatag ataattggat
    31981 gtgtattgtt cacgatagaa tacctacttt accgatgaca atatctaatc aactaggttt
    32041 ctttgatgat ttgcaaatta atgtaaaaaa acgtattgaa aagattagaa cattatcacg
    32101 aagaagtcca agaatcaata tgaattttca gatggatata aaaaatacag gtaaattaag
    32161 aatagtaaat tttaattatc aatatgtatt attaaatttt gagaataaaa atatttatcc
    32221 taaagaaata gcattggaaa ttaatgaaaa tattgtgtta gaatgtgatt tatgtgaggg
    32281 taaaatcgat gaaagaggtc tcttatataa agtaaataac attgacagca taatgcaaaa
    32341 tttcttttta agagatgaaa tgatggattg gatattacaa aataagacaa ttcctgtttc
    32401 aaatcctagt gaaaagaagg aaaaaagtat agatgagttt gaacccatgg aatatattta
    32461 ggagagatga aatgaagact ttaaaagtgt ttttttgttc tgcaaaatat cttattttgg
    32521 tgtttatttt ttttataaat actactttta taagttcagc gacaagtgat aaaattttag
    32581 atttatcctt taaaaagata gagactgatt taagttctag gattacatat gaagatacag
    32641 gtgtaaaaat agaaacagat agttctaaaa gtgataagga acgttattta tatatttacc
    32701 aaaatataaa agaaaattgg agtatgtata ataattttta tattgaaatt caaaataaaa
    32761 ataaaagttc ccagaaaata aatttaagta tacaaagtaa gaatatgctt gaatttaggt
    32821 taaaagaagg tagtgaaata tttttggaag ataaaaatat aatatattca gataaaataa
    32881 aagaaggatg catagaagta ccaggtgaat ttgaaggcaa aatttatgtg aactttaata
    32941 gccttataaa tgagaaaagt aatgttgtgc tggactcaag tatgctttca aacattgtta
    33001 gttggggaat tacatttatt ccatctgatg aagaacataa tattgttatt ataaaaaaga
    33061 tatctttatt gtctgaagag aaattaagtt ttttacataa tattaaaata actggcaatg
    33121 aagaagtaca aataccagta ttgggtcaaa gtataagtca gtatgaggta ttaggtctta
    33181 aacgtgattc taaaattaag tattctctta tgggaaaaca agataatgtg agcatatctc
    33241 aaaatggtaa attaacatta agtaataaag ccgaacctgg acaaattatt ttacaagtta
    33301 atgttgatga taagtttaaa attggaagga aaatagcatt aactgaatct tggagtatta
    33361 ataaaaaaga taaggatggt gttccatata cattggtatc tcctgagcaa tcaccaacag
    33421 tacaatatat gaaaaaaata aactttatga ataacatcat aacgtttgtt cgaattttat
    33481 ttgtatctct agtaataata tgctttggaa tatatttata ttggaaaaaa tgttcaaaaa
    33541 ctaagtaaag agaggaggtt agattgtgtt attttcaagt ttaatttttt tattttattt
    33601 tttacctata actttggttt tatattatgt atttaggttt aaccgaacga tacagaacat
    33661 gatattgctt gctgttagtt tattttttta tgcttgggga gagccaaaat ttgttgttat
    33721 catgatagtt tccataataa tgaactatat atttggttta ctagtagata gatataggga
    33781 aagtaaaata aaagtaaaag tttttttagt tttgatgtgt gtatataata taggtgtttt
    33841 atttatattt aaatatctag cttttgcgct aagaaatatt agtgaactta ttaatactga
    33901 actaactata cctagtattg ttttacctat aggaatttca ttttttacat ttcagggaat
    33961 gtcttatgtt atagatgtat atcgtagaca tggagaagtc caaaagaatc ctttttatgt
    34021 gggactgtat atagcatttt tcccacaatt aatagcaggt cctatagtta gatatgagag
    34081 tgtggcagaa caaattctca atagaaaaga aacttggaat aaattttcta ttgggacttg
    34141 caggtttata acaggtctag gcaaaaaagt attaatttca aataacatgg ctatagtagc
    34201 agactatatt tacactatga atagtcaagg agaaatagct gcatcacttg catggcttgg
    34261 ttcaatagcc tatactatgc aaatattctt tgatttttct gcttattcag atatggctat
    34321 aggtttaggt cttatgtttg gttttaaatt tgaagagaac tttaattatc catatatatc
    34381 aaaatcaatt agtgagtttt ggagaagatg gcacatttct ttgggaatgt ggtttaagag
    34441 ctatatatac tttccgcttg gaggttctag agtagcaaat aaagatatta tgattagaaa
    34501 tatgtttata gtgtggttat ttactggaat atggcatgga gcagaatgga catttgtaat
    34561 ttgggggatt ttaaactttg ttttcttgat tatagagcga tttataatgt ttgaaaagat
    34621 tgaaaatcac aattttataa aacatattta tgcgttatta gtagttaact ttgggtgggt
    34681 attgtttagg tcacctaatt taaaagaagc atataattat ttcaaagcta tgcttggagg
    34741 caatggagtt atatggagtg attatacata tatgttttta aaagaatact gggttttctt
    34801 tatatttgca tttattttta gtgtacctat tgcaaagaaa atcaataagt ttgttgttga
    34861 acaagctaaa tatagtatga tatttaacgt attttatcca atctcagcta ttttattgtt
    34921 ctttatttct gtgacatatt tagtaactgg aagctataat ccatttattt attttaattt
    34981 ttagttggga ggaaaagtat gaatagattt tttaaagtaa gattaataac tgcaattatg
    35041 ttcttaatta ttttattcgc tttctctatc tttaatattt gttattctgg aaaagatatg
    35101 atacaggaga taaaaaattc agttgctgaa aaaagtagtt taaaagatac aatatcatct
    35161 attgataaca cagtaaatga caaagtaata tataaaaata cgttcattga aacttatggc
    35221 tacttacaaa agttaatggg taaaaatgag ttttcaaaat ttagtgttgt aaaagatact
    35281 tctggaaaga tgcattatac atattttgct aaagctccaa atccagttgg tgtgatagca
    35341 gatagggtaa gaaaattttc tgaggaaatg gaaaagcaaa acactaaatt agtttactta
    35401 atgactccag ataagtatat aaaaggagtg acaaaatttc caaaaggaat accttattct
    35461 tatagcaatg agacagcaga taatttttta aatcaactaa aaaagtataa tgtggactat
    35521 attgatttta gagaaaatat tttaaagagt ggtattccaa aagatgattt attttttaaa
    35581 acagaccatc actggaaagt agaaacttct ttttgggcat ttggagaatt agttgagcaa
    35641 cttaataaaa aatacaatat gaatttagat gaaaatcatt actatagaga taaagaaaat
    35701 tataactcaa ttgtatatcc taagtcattt cttggttcta tgggaagaaa gactggtatc
    35761 ttatatggtg gtatagatga ttttacatta atttatccta aatttaagac taattatact
    35821 tattatacag attctaaatc tcaaaaattt gaattaaaag gaaggtttga agagagttta
    35881 atattatcat atccttttaa tgcagacctt gatttaatgg atggtcaatc agataagtat
    35941 tttacatatt tattggggaa tagaccatta gttaaaataa aaaatataga aaatccagat
    36001 ggattaaaag tgctgtttgt taaagattct ctaattgtac caacagcagc atttttttcc
    36061 tctgtttgtt catctataga tatgatagac cctagatatt atgatggaga tatactagaa
    36121 tatgcaaaat cccatgacta tgactttgtt tttatatctg tatatcccca aaatcttaca
    36181 aaagaatttt ttccgttttg taagtaaatt tctaaagaaa ttttcttata aaataaaaaa
    36241 taaatggcta actagttaaa agttagccat ttatcttaag tgttactcta agaaatgttt
    36301 tttttaccaa atacttcttt atctattttt ccaagtgatt ttcctacagt aattgatgat
    36361 accatagcac cattaacatt tatcatggta cgtcccatat ctagtatagg gtctattgca
    36421 agtataccac caactaatgg aaaataagaa ccaagaccaa ctccagagat aacaactgaa
    36481 acggccattg tagcagttcc tggaagacca gctattccaa acgaactaat tgttataaca
    36541 attaacaaca taacataaag gctcatatcc atgtttactc cagccatatt ggcaagtgtt
    36601 acagccatta aagcaggata tatacctgca caaccattca tacccatatt tgcacctaaa
    36661 cttgctgtaa agcttgatac accctcatca actccgtgat tttgtactag actttctatc
    36721 gttacaggca atgttcctaa actagaacgt gaagtaaatg ccagaagtaa agcttcagtt
    36781 gaatttctta tataagtaat gggatttaga ccatttaaag ttataaaaat taaatgtatg
    36841 gtaaataata ttgccactga tatataaaga gctaatataa actgtaaaac agatactatt
    36901 gagctaatac cttgtgaagt tattgtattt gcaagcagag caaccacagc ataaggcata
    36961 aatttaataa cagtcataga cacacttaca attattttgt aagatgcttc tataaaatct
    37021 ataaatggtt ttattgtttc aaagtgtttt ttacttaatc tctttattga ggtagctata
    37081 aatgtagcaa atattattgt agctacaata tttccatttg ccatagccat tacaggatta
    37141 gatggtagta atcctcttaa agtatccaca agagagttta tttctttaag ttcttgattt
    37201 tcaactaaag taggcatatt agctcctaca cctaacttca ttatatttgc aacgataatt
    37261 ccaacaattg cagctatagc agtagtagca agaagcatac ctaaagattt aaatgtcaga
    37321 gttccaagat tgttatcttt catatttata attactttta taatggataa gaaaactaaa
    37381 ggtactacta acatttttaa taagtccata aatccagaac caaataggct ataccacttg
    37441 cttacttctt gtagccaagt gacttgagtg gtatcattag gaaaaccagc aaaagcttgt
    37501 attatgattc ccaatatgag tcctagtact gtagcatata ttgttctcaa tgaaaatttt
    37561 atttttttct tttcaaattg tctaacaata aagaatgtac ctagaagtaa tattatgaaa
    37621 aaaatcgttt taaaatctgt aatcataaga aattgtgata aaaatgaact ttccaatatt
    37681 ttttctcctt ttatttaaaa ataaattttt ctaagtttaa atgataccaa tatattaaaa
    37741 aaatgtcaat attatatcac aatttactta aatagagtaa aataaattga ttttgaaaaa
    37801 tgtgattaag tatagaaaaa tagggtataa taaatgttta aagaatttga attgtggtaa
    37861 gaaaataaaa aatagctttt ataatatttg atttcaaatt aaaatatgtt aaataaataa
    37921 ctaaaaattg ttatgaaaaa tgtttacata gaatatatat caaatgaaaa gcttaaaata
    37981 agtgaagaaa ttaactagac atcaaaggag agataaaatg agagtagtaa taattggtgg
    38041 tgttgcagct ggtatgagtg ctgcagcaaa attaaaaaga ataaaacctg agtatgaagt
    38101 tgttgtttat gaaaaaactg aaatagtatc ttttggtgct tgtggattac catactttgt
    38161 tggtggtttt tttgatgatg ctgatgaatt attagcaaga acaccagaga aacttagaga
    38221 agctggaatt gatttaaata tatttagaga agtcgttgag gttgatagtg aatctaagaa
    38281 aataaaagta aaaaatataa aaacagatga aatatatgaa gattactatg ataaattgat
    38341 gatagcaact ggagcaagaa gtattatgcc tcctataaaa aacataaaat tgaagaatgt
    38401 ttctacatta aaaagtttat atgatggaga atatctaaaa aaacttttaa gtaatgaaga
    38461 taataaaaga gtgacaataa taggagctgg ttttataggg ttagaagcag ttgaggcatg
    38521 taagaaatta ggtaaagatg tagatgtaat tcagttagaa gatagaatat tgccacaagt
    38581 atttgataag gagatgacgg atgtattaga agaagaaata aatagacata atgttaattt
    38641 gcacttggat gaaatggttg tagaactttg tggagaagat aaagtagaaa aagttataac
    38701 aaacaaaggg gaaatagata cagatgtagt aataattgct actggtgtga gacctaatac
    38761 cgagtttttg tcaaatacta atataaaaat gctaaaaaat ggagctatag tagttgatga
    38821 atatggaaga acttctgtag aagatatata ctctgctgga gattgtgcaa ctataaagaa
    38881 tatagtaagt aatgaaaatg tatatgttcc tttagctact ggagctaata aattaggtag
    38941 gatagtagga gaaaacctag caggaagaga agtatcttat caaggttcac tatcttcaag
    39001 ttgtataaaa attatggata tggaggcagc atcaactgga attacagaaa agcaagctaa
    39061 agatttaggc ataaatgtca aatctaaatt tatttctgat tataatcaaa ctcattatta
    39121 tccaggaaga aataaaatat atgtaaaact tatttatgat gcagatacaa aagttatttt
    39181 aggcggacaa gtggctgggt ttaaggatgc tgtccaaaga gcaaatgtat tggcagctgc
    39241 tatctttgga aaaatgacca caagtcaact tggaatgtta gatttatgtt atgccccacc
    39301 atttgcaaga acatgggatg ttttaaatgt agctggaaat gtgtctaaat aatatttaga
    39361 ttatagctaa tatagttgac aagcaattaa gggaatgaat ttacctaaac tatttttatc
    39421 atggtaaaat tcatccctaa atttttattc caaattttta gaaataagga ttccattaaa
    39481 ttattcatca aaatttaaac gcaagcttaa tctatcttct ccaagaatag tattttcaaa
    39541 aacattagta gcattttcta aatacatact aggtatgtct aaagaaggac taaaatgggt
    39601 cagtaaaagt tgtttgacat ttccaagttt tgcaagatta gcagcttctc taaaagtcat
    39661 atgtttgttt ctaacagctt ttgatatatc taaatcatca ccatacatgg cttcacatat
    39721 aaataaatca ctatctttta tgaaatctgg tattgattct ataggtcttg tatcggttgt
    39781 aagacttatc ttaactccct ttctcaattc tcctaagacc atatctggag tgtattgttt
    39841 tgaatttaaa actatatttt gaccttcttg gagtttgttc cataatattt taggaacttc
    39901 gtttttagtt gccttatcta tatcaaattt agggtttctt ctaaaattaa aactgtatcc
    39961 taaacactct atagagtgtt ctaaactgat tgttgaaatt tctaaatttc ttaaaacttt
    40021 attatctaaa gaaaaattgc cttgagggtt ttctataatt tttaaagtgt atggtacata
    40081 ttctactaaa tttctcatag aacgtataca atcgactatt ccaactggac caactatagt
    40141 taaatcactt gtacgaccac tattcccaat tgttgataaa agtcctaaaa gtccaaatat
    40201 atggtcacca tgtaaatggg ttatacaaat caagtctata tctttaaagc cacatttttt
    40261 tagcttcata gatacctgag taccttcacc acagtcaatt agtatttttc tacctttata
    40321 gtttataaat acagaggata gaaatctgtt tggcataggt acatttccac cacatccaag
    40381 taaagttaaa tctatcattt tttccccttt ctaaaattaa cacaaatctg ttgtttgttt
    40441 ttattatgat atcctattat aattatttta acattaaata taacaataaa tgtgtatttg
    40501 agtcaagaaa tattatcaaa taaagttttt atatagcaat gtataaaaat ttggagaacg
    40561 aaaagtgaaa attatattta ggagtattta aaggaggaaa atttaatgaa agtattaaag
    40621 aaaattacta ttttgtcatt gattacatta accttagttg gttgtagtaa cggagaagat
    40681 aaaaaagtgg ttgaaggttc taagaaaatt caaaattcta aaaaagatga acagcctata
    40741 atagtacata aagatgcaaa tatattgaaa gataggtatg agccaccaaa aggatttaaa
    40801 agagttgatg tagaaaaagg tagttttgaa tcatttctta ggaatactaa gctaaaaaat
    40861 tatggagaga aagtaaagta ttatgatgga agaattaagg aatcaaaagg tatttatgat
    40921 agtgtatttg atgttgatat tggcaataga gatttgcatc aatgtgcaga tgcgattatg
    40981 cttatgagag cagaatactt gtatcagaat aaactatatg atgaaataag ttttgatttt
    41041 gttgatggtt ttaaggcgaa gtattcaaaa tgggcacaag gatatagaat ttcagtaaaa
    41101 gatagtggtt caagctggta taaagcaact gaatattcaa catcttatga gagtttcaga
    41161 aagtttatgg atatagtgtt tgcatattca ggaacgttat ctcttgaaaa agaattggag
    41221 cctgtaaaag ttgaggatat gaaaatagga gatgtgttta tagttggtgg aagtccagga
    41281 catgcaagta taattatgga tatggcagaa aatgaaaaga ctggtaaaaa ggtatttatg
    41341 atagcccaaa gctatatgcc tgcacaacaa acgcaacttc ttattaatcg taaaaatcca
    41401 aatttgagtc cttggtatag tttagatttt gaaggtgatt tgacaatacc tcaatggact
    41461 tttaaaagag accagttgaa aagattttaa aataataatt ttatataata aaagaaaaat
    41521 atagatgtga aaaaatgtaa ttagattaaa actaattgga tatggacaaa attaaattga
    41581 tagataaata ttaaaaatta cactaatcaa tcttactata gtttaatatt tatctaaaaa
    41641 gttttatgta aaatataaat tttatattaa agctaaaact aatttgagga ggctaattta
    41701 tattgaagat tagaaggtat ttaattttgg taattataat tctaattaca acaagtcaga
    41761 gttttatttc ttatgggcta gagcgatttg aagaaataac catttttcac actaatgata
    41821 tacatggaag atatgctgaa ggtgatgacc atatacaaat aggaaatcta gccacattga
    41881 aaaaagaaac tccaaactca attttagtag atgcaggaga ttgtttgcat ggacttccaa
    41941 tagtaaatat ggatagaggg aaaagtgcaa ttgaattaat taaatgtgct ggatatgact
    42001 atattactcc tggaaatcat gactttaatt atggaaaaga tagactgttt gagttatcta
    42061 aaagtgctaa tttaggagag aatactttaa aatttttatc atcaaatatt tttgaaaatg
    42121 gaaaaaaagt ttttgatagt aatgatataa aagaaataga tggaataaaa ataggatttt
    42181 ttggactctc aacccaggaa acaaagaaca aaactggatt taaaaatgtg gaaggattag
    42241 agtttaaaaa tcctatagaa agtgcaacag aacaagtaaa tgaattaaaa ggaaaaggtg
    42301 ccaatgtaat agttgcaata tgtcatattg gtacaaatag tttaagtaag ccaaatagta
    42361 tagatatagc aaatgaggtt caaggaattg atttgattat tgatggacac agccacacta
    42421 agtttgaaaa tggtaaacaa gtgggtgata cattgattgt aagtgcagga caatatttag
    42481 aaaatattgg cgagataaaa ctaactttgg atactaatga tgtagaaaat ataaaaatag
    42541 aaaataaatc agttagatta attggaaaag aagaagcatt aaagtataag cttgactacg
    42601 ttgtaagtaa aaaggttgat gaaataaaag aacaacaaga aaaaatatta gataaagtta
    42661 taggtgcaac tacaaatact ctagatggtt catatgaaaa tgtaaggaca aaagaaacta
    42721 atttgggtaa tttaataagt gacattttac tagataaaac taaggctgat atttctttat
    42781 ttaatggagg gaatattcga gatacgatag aaaaaggcaa tataacaaga agagatattg
    42841 tagatgtatt tccttttagc aatactatag ttaccaaaga acttacagga gcacagatta
    42901 aagatgtatt ggagcatgga gtaaaacttt atccagaaaa aagttcagcc tttttgcaag
    42961 tgggaggaat ttcatactac tttaatccta aacaaaaaga aggcgaaaga ataacaagta
    43021 ttcaaaaaga aggaaagcca ttagatttaa ataaaaagta tgtagttgca acgaatgatt
    43081 atatagcctc aggtggagat gaatttcctt gtttttcaaa agagccaata ctaaaggaat
    43141 ttggaaattt agagagtgca gtcattgaat atatagagta caaaaaagaa atatcaaaaa
    43201 atgtagatgg aagaattggt attaaatcta aagatgagtt aataagcaag aatcatgaga
    43261 gtgatttgat attaagtgaa aatgaaaaaa atatcaatga taatgaagag aatctatcta
    43321 ataatataaa aagctctgat agtgacaaaa ataaaagctt agataaaaca agtgctagag
    43381 aaagttctaa tcaaggaata tctaaaagag aagctaaaga aaaatctcct aaaacaggag
    43441 atttaggttt ttcaaatagt ataattatat ttatagtttc aagtacattg atatgtttat
    43501 tgaattttaa tcaaaaagag ttaaaggata aaaagtcaaa ataaaaatta atgagcaaaa
    43561 ttagtagata gtaatttaat tttagataat agtaaacagc aaaggaaatt agttatataa
    43621 atactaattt cctttgtttt atgctttaac atgattattt ttaaataaaa ttgtatttta
    43681 tgaaaaataa ttacactaaa tggcatagta ttatggggat ttttagtttt tattcaagta
    43741 aatgaaatca tattaacaac ttctgatata tttacaaatt gataaaaaaa tgattttatt
    43801 agtatgatat aatttagcta gtatatatga tgatggagga tatagggatg aataatatat
    43861 tacttttgga agatgacaaa tctttaaata gaggaattag ttttaaatta aaaaaagaag
    43921 gatacaatgt ttttagtgca tttggtatag aagaagcaaa aaatattttc gcaaaagaag
    43981 agatatgctt aattatttcc gatataggtc tacctgatgg tagtggtttt gacttttgtg
    44041 aggagattag aaagaaaagt gatgtttaca taataatgtt gacagctctt gatgaagagg
    44101 tggatattgt cacaggatat gatttaggtg cagatgatta tatcacaaag ccttttagct
    44161 tgatggtatt aatatcaaaa gtgaatgcgc ttatgaaaag agtaaataca gtaaaaaatt
    44221 atactctatt agtttgtgat gatttattct tttattatat agaaaataaa ttaattataa
    44281 gaacagatga taaggaagaa gagataattt taagtaagac agagacaaaa ctattaaaat
    44341 atttgatgga aaattctatg cagacattga ctaaagaaca actgttagaa tctttatggg
    44401 atagtagtgg taattttgta gatgataata cgattgcagt aaatataaga agacttagac
    44461 aaaaggttga aaaaaatcca tctgaaccta aatatataaa aacagtaaga ggggttggat
    44521 atatgtgggg agaaaggagt ataaaaaaat gctaaaaaat atgaacctca aagatggttt
    44581 aagtgtgaaa gatgattata taatgagaag agttattgta tattctgttt tatctgtact
    44641 aatagtaacc cttgtattta tactttttga atatagcaga ttacataaat tgtatgaaca
    44701 tcaaataaaa aatactcaaa atattgctgg aatattgatt gaaaaatatc ctaatgatga
    44761 aatagatata gtaaattcta tttataattc cgattatagt aaggtgtcta ctggagaaaa
    44821 ggcatttaag aaatttggct atggattgga aaataaaatg tcaaatgata aaaattttag
    44881 tttatactta aaatcttttt ttaaggaatc attttttatt tttttaatga tgtttatagt
    44941 agttataggt ataatatatt attttataag atacataaat aaaagattga gtaaaattta
    45001 ttttatagta gataagatgg cacaagaaaa ctacttgaag gacactactg agtcagaaga
    45061 aactggttat ctagtagaat ataatgagta ttttaaagaa ggtacattta gtaagataaa
    45121 taactgtcta tatgagctta atagaagttt aaagataaag tttattaaat tagaaaaaga
    45181 aaaagaaagt gtaaaatcat tagtaacaga tatttctcat caattaaaaa ctcctttagc
    45241 atccttaaaa ttatacaata cattacttat agaagaaaaa ttagaagaag atgaagccaa
    45301 cgagttctta ttaaccaata aggcttctat aaataaatta gaaaatttaa taaattcttt
    45361 agtgaacata tcaaggttag aaattagcat gataaacata aaaaaagaag ataatgatat
    45421 aaaaaatact attctaaatg cagtaaaaag tgttacccct aaggctaaga gcaaaagtat
    45481 aacattaaat ttaaatgaat ttaacagtag aattatacca catgataaaa agtggactga
    45541 ggagagtatt tttaatgtat tagataatgc tgtaaagtac acacaggtaa atggagaaat
    45601 aaatatatct gtagaagaaa ctgctaattt ctttaaaatt ataatagagg ataatggcat
    45661 agggattgaa aaaaatgagt ttaataatat atttaaacgt ttttatagag gttctgctga
    45721 tgaaattgaa tctgtagaag gtagtggggt aggattgtat ttaagtagaa agattttaga
    45781 agaacaaggt ggaaatatta tagtttcttc aaaagtaggg atagggagta agttttcttt
    45841 gtttcttaca actatgtaat attaaatgaa aggaaaatgt aagcctctac atttattata
    45901 taagtataaa cagctccaga aataaaaaat taataaaata tggggtaata taacttagaa
    45961 tgaggggata attttatgaa aatactatat actgaaaatt tatcaaagca ttatggaaaa
    46021 ggagaaaact tagtaagagc tttggacaat gttaacttag aaataaatga aggtgaattt
    46081 gttgcaataa taggaaaatc aggttctgga aaaagtacac ttttacatat gattggaggt
    46141 cttgatatac ctacatctgg gaaggtgtat atagataata aaaatatatt tacattaaaa
    46201 gaagaagaac tagctgtgtt tagaagaaga aaaatagggt ttatatttca aagctataat
    46261 ttaattccat ctcttaatgt atgggaaaat gtagtgttgc caatcggtct agatggtaga
    46321 gaagtagatg aatcttttat aaaagaactt ttgaaatcat taggacttga aaataaacat
    46381 gacgttttac caaatacatt atcaggtggt caacaacaaa gggtggcaat agcaagagca
    46441 ttagctacaa gaccagctat aatacttgct gatgaaccaa ctggaaacct agattctaaa
    46501 acatctgatg aggttatgag tatattaaaa tctatgtcta agaagtacag tcaaactcta
    46561 gttatgataa ctcatgatga cagtatagcc caaatggctg atagagttat attcatagaa
    46621 gatggcaggg tgtcaaaggt tggtgataaa aatgattaat actactaaaa tagcaaaaag
    46681 taatttaaaa caaaataaat caaaaagtat tctaataata atcactataa tactttctac
    46741 tttaatgctt tcttctattg gaatttatat tgttgatgca ggagcatatc aaaaagaaaa
    46801 tactataaag tattcaggta attatcaagg tatattagca aatgtagatg aaaaacaggc
    46861 tgatatattg agtaatcatg cagatgttga attaactggt gaaatgaatg gagtaggtgt
    46921 tgaaaaactg gaagatgatt caaatatttc tctagcatac atgaatgaag atgctttgaa
    46981 attaaatagt tttgaactta tcaaaggaaa attgccaaca aaagagaatg aaattgtgtt
    47041 agatagtgga gctttaaagg cacttggtta taaaaataaa gacttaggtg aaaagataaa
    47101 aatatcttat aatgactata aaaatgataa aaaaattgaa aaagagttcg ttataagtgg
    47161 aatcttaaaa actagcgaaa tatctgaagc aggtaaatat tattatgcta ttatatcaga
    47221 attatatatg agaaatacta gagatatgtc acaagaagat tttaatattt atgtaaaatt
    47281 taatgataaa agtaatttat caatagagca agcaaaagaa aaattagata aaattgctaa
    47341 tgatatagga cttgatacta ttaatactgc tgtaaatgaa aattatataa atgctttaaa
    47401 accagatatg gaaacgatta tgggtggagg ttttgtagga ttagtaattg tattatctag
    47461 tattttagta atatacaata tattttatat ttcaatagtt acaaaagttc aagaatttgg
    47521 aaaacttaga gcaattggtg caactaagaa acaaataaaa aatatagtgt ttaaggaagg
    47581 gtttatatta gccggaatag caataccaat aggaataatt ttaggctatg tacttgcaaa
    47641 tattataata aaatcattta tggatataga tgtaaaatca tcacgattac cagttatatt
    47701 atcggttgtg gtaataagct ttatatctgt tgttttatca cttttaaaac caatgaaagt
    47761 agcatcaaaa gtatccattg ttgacgcagt aagatataca ggaaataaaa taagtaataa
    47821 gagtaagaga aaaggatata aaaatataaa tctaaagaga ttatcacatg ccaatttaga
    47881 aagaaataaa aaacgtactt acatgacatt ggcatcactt atacttagtg gaactatatt
    47941 tataacagta tcaactgctt tagaaagttt tgatgctgag aaaatggcaa gagaacactt
    48001 tccttatgat atagaagtta gactatctgg ttatgaaatg aacagtgata aaaatcctaa
    48061 aaataatttg aatattttac aaatggataa tcctctaagt aaagattttt ttaatcaaat
    48121 aaagaacatg gaaggaatta aaagaataga atctgcaaga agtgtaaaaa ttggtatgga
    48181 ggactatgat gttgaattta agtatgacct tttacaaagt ataaatgaaa atgatgtaaa
    48241 atcattaaac aagaatctaa tagatggaaa aataaatctt gaaagactgc aaactggaga
    48301 tgaaatagta attactcatg ttgatacagc aaaagaaatg ggagtaaaag caggagataa
    48361 aataagatta actttatatg atggagataa aaagataaaa aaagaattta aagttcaagc
    48421 aatagctatg ggggttccaa gttttggtat agggaaggat tttattgata gaactttaaa
    48481 atacgattca actagtagct tgggaatata tacagaagaa ggaaagtatc aagaaatcaa
    48541 agatagtatc aagaagattg ctaaaaataa tggattttta gagactgatt ttatagatag
    48601 tagaattgag tcaaataagg ctgttatttc atttataaag ataatgggat acacgctaac
    48661 aggcataata ggtgtgattg gatttatgaa tcttgtcaac acaatgataa ctagcatagt
    48721 tactagaaaa aaagaacttg gtatgcttca agctattggt cttactaata aacaattagt
    48781 gaaaatgctt aattcagagg caatgtatta cacttctgga atgatgatag gaagtatttt
    48841 atttggaggt atattagggt atattgctgt tatatttcta aaaaagactg gattatctta
    48901 tgcaacatat tctttgccaa tagtaccaat cttgttgatg attgtgtgta ttcttatagc
    48961 tcaatttata acaacttatt tgataggacg aagttttaat aaagaatctc ttattgatag
    49021 agtaagatac agtgaataaa atgcaaatat ttgtggttag tttaactagc aaaaagcata
    49081 taaaaaatat agttaaagag attcataatt aaataggtta aagcatagta cagagcaaac
    49141 aatttaaacg gatgtttgct ctgtatttta ttatgtaatc aataatatat ctaatatact
    49201 gatattattc atgttagaaa atgtatcggt acaattctaa aaaagtataa aattgtacca
    49261 tataaaatga aaattttgta taataatcgt atagaaaata aataggagct gatatatatg
    49321 aataagaaga ttaattatgc accaggacca actgaaacta gggaaaatgt aagattagca
    49381 agagcagaaa aaacaacaaa tccagatata gatattgatt ttgttgaatt ttataaaaag
    49441 acatgcgaga agtttggcaa tatagttgga acaaaaaacg atgtttacat attgagtggt
    49501 gaaggaatat tagggcttga agcagcatgt gcatcactta ccgaaaaagg agatagagta
    49561 cttgtaattg acaatggtat atatggagaa ggttttaaag attttgtaac aatgtatggt
    49621 ggggagtatg tgcttttttc aagtgaatat actaaaagta tagatattga tgaattgaaa
    49681 gcatttttag atgaggatag taattttaaa tatgcaacag tagtgcattg tgatactcca
    49741 actggcgtat taaatgatgt aagcaaaata tgtccattac ttaaagaata tggaatatta
    49801 actgtagttg attctgtagc aggtatggta ggagaaagat taagtgtgga tgaatctaaa
    49861 atagatataa tattaggtgg ttcacaaaaa gctatttctg caccagcagg acttactata
    49921 gttggaataa gtcaagatgc aaaaaattgt ataaaaaata gaaaaactga tgtaataggt
    49981 ttttattgta atttaaatat atgggaaggc tattatgaaa aaaaatactt cccttataca
    50041 atgccaataa gtgacataat ggggcttgat aaagcacttg ataatatatt agaagaagga
    50101 atagaaaatg tattagatag acatgaaaag atagcttata gtgttagaaa atctattgag
    50161 gaatatggtc tagaactatt tttagaagaa ggatattcta atacagtcac agctataaaa
    50221 attcctgaaa gtataggagc tttaaaactt actgattata tgcttaaaaa ttataataca
    50281 cttgttgcaa catcactaaa tcaatatatg gatactattt taagaatagg tcatatggga
    50341 gagaatgcaa atctaaataa aatagagcat gtactaaatg tcttggataa aagtttaagt
    50401 gcattaggat tcaaaggaaa tgaaaatttg ttgaatttat ttaataagta ttatttttaa
    50461 tagatagggg gatatagtat ggaagttaca gctaagcaaa aatttacaac gaaagacatg
    50521 gtggaaacct cactattaat agcattagta tttattgcaa ctaaatttat aaatataaga
    50581 ttaccaatat ctataaatgg tgggcttgtg catttaggaa cagcaatgtt gttcatatca
    50641 gcaatagtat ttggaagtaa aaaaggtgct ttatcaggag ctattggtat gtcacttttt
    50701 gatttaatat ctgggtggac tttatgggca ccgtttacct ttatagttag aggagttatg
    50761 ggttatttac ttggaaagat tgcatgggca aatggtaata atgggaataa ttttttaatc
    50821 aatgtaatag gaatatgtgt ctcatcaatt tggatgttat ttggatatta tataacagaa
    50881 gttatacttt atggtaattt tatagtacct cttacttcaa taccaggtaa cttaatgcaa
    50941 gtattgatag gtcttataat agcgcttcct atttctaagg tattgaaaaa atgtataaaa
    51001 taaatataaa gtgaactaaa atttatgata gcataaatta tactgtataa tattaagatg
    51061 gagtacataa ttaaaaaatt ttagtataga ggttagttca caatgttatc agttacaaga
    51121 tattcaatca aaaatgttcg tttaaagccg tgggtaaagt ttatttggta ttttgaaaca
    51181 aaatcaaata tactgttaaa taataagtta cttcctacag acagtataga tattattctt
    51241 aatctttctg atgttatgga atataaaatt gaaaatcaag actatactgc tagtaatatg
    51301 cattttaatg gaataaggga taaacatgga tttattattc aacatggaaa tatacgtgtt
    51361 atagggattt ctttttatcc atttggtctc tatccatttt taaagatacc aatttcagag
    51421 tttaacagac aaattgttga cttagaagca gtatcagaac tatttgccaa aaaattggaa
    51481 gagtcattaa atccaacaca atcagtagaa aaaattgttt tatatttaga agaaatgtta
    51541 ttatccatat tagaagaaga tttaatttca aataaatctg taaagcttct aaattcattt
    51601 atatatgata atagatatag caatataaaa gcattttgtg ataatacaaa tataaatatt
    51661 aaaacattgg aaagaatctg tttaaaatat acagggtata cacctaagat tttaaagaga
    51721 atttacagat ttaaaatggc aagtagtcaa cttatttata attataaaga taatgaactt
    51781 tttgatttta tttatgaaaa tgagtactat gaccaagcac actttataaa agaatttaaa
    51841 aaattttcag ggacgtcacc aattaaattt attggtgaga ataagacgat taaagaaaat
    51901 ataaagtaca actatttata attatactgt cgatttttta caatacctcc aatttatttt
    51961 ttgatatgat ataggaaaat aaattggagg ttttacatat gaaaattatt acagaaatag
    52021 ttgagtttaa tgtggaagaa aatttatcaa aagaagagtt tatagaaatt gtaaatgact
    52081 tagaattaaa atttcattct attcaatctg gttttattga tactgagctt ttatatgatg
    52141 aaaagtctca aaagtggatt atgatacaac attgggaatc tatagaagac ctcaagagtg
    52201 cttctagtaa aatgtttaaa gattcttcaa ctgagaaatt tagaaaagct ttaaagaatc
    52261 aaactgttaa gatgaatata ataccacaaa ttaaaagttg gaaattatag aactgaatgt
    52321 aaaagatagt taaatagtat caaactaaat tagccaaata acattttgta tttaaattgt
    52381 tatttggctt ttagtgttta ctttaatatg agtgttatat actagaaaaa ccaacaaact
    52441 gttagagtgt ggattataat aatgtcatat ttgggagcta aaaggcttaa tactgatttg
    52501 tcaatatgtc tttttagcat gttgaacagg tatattgcca tgctttcatt attttatttt
    52561 tttgccttat actggactac atttgcagtt tcctaatata tttggctcat atttaaatgc
    52621 cattatctaa aagtactatt gctgtacgag aggcaatagg cattttagaa gaaggaggag
    52681 ttgagctaaa gtatacatca taatctctta agttttcttt ttttgtgttt cctccaaact
    52741 ctaccatatc agtatttaat atcgttttgt agtttgtatt ttgaaacttt gaaatatcaa
    52801 ctgattgtat tagattaggg tggaaattaa atataaataa atatttgccc ttactgtaca
    52861 ccaaaatctt tttatcatta tccatatgta taagttcatg actattttgg ataaaaatat
    52921 cactgtattt tgtaagttcg agcatagatt tatcaaaatt taataattgt ttatacttta
    52981 agttgttatc ttctgaaagg ctccattgtc ttctggcata cttatagctc caattgttgc
    53041 cttctcgtgg aaaatcaatc cactcaggat gtccaaattc atttcccata aaatttagat
    53101 atccttcacc tgctagagaa aaagttatta atttaattaa tttaataaga gatattgctc
    53161 tattgataac atggttattt gagtttattt ccatattcca gtacatttcc ttgtcagcta
    53221 gccaaaaaat aattgtttta tcgccaacta atgcttggtc atgggattca caatatccta
    53281 tatttttttc tccaggtcgt ctagtagtaa gttcatacca catttttccc aaatcccagt
    53341 cttcatcaga taagttagag atagttttta tccaaaaatc aggtactccc attgcgagtc
    53401 tatagtcaaa tccaattccg ccgtctttta tagggataca cataccaggc attccactca
    53461 tatcttctgc aatacttatc gaattaggtt taatttcttt tattaattca tttgcaaatt
    53521 ggagatatgt tatagcttca atatctgtat tcatactaaa atatttttca taactatcaa
    53581 atgaaactcc aagaccatgg ttatgatata acattgaagt cacaccatca aacctaaagc
    53641 catcaaaatg gtactcattt aaccaaaatt ttatatttga aagcaaaaaa tgtattactt
    53701 caggtttacc atagttaaaa agttttgtac cccaagcagg atgatttccc tttgaacctg
    53761 agtggaaaaa ttgatgttca cttccatcaa actcatttat tccttctaaa gtattcttta
    53821 cagcatgaga atgaactaaa tccaataaaa cagcaatccc cattgaatga gcagtgttta
    53881 tgaggttttt taagtcttca ggagtaccaa acctagatga gattgcataa aagtttgaaa
    53941 cttggtatcc aaaagatgca taatatggat gttccataat agccataagc tgaatggtat
    54001 tgtatccagc tttttttatc ttaggaagta ttttttcagt aaattcatta taagttccaa
    54061 tagattcgct ttcagtagac atacctatat gacattcata gattagtgga gaagttatgt
    54121 tttttaagtc aaaattattg tcagtccaga taaatggagt ttttggttgc catatttgtc
    54181 cattaaatcc accaaaatct ttttgaatta ctctttttat atataatggt atacggtcaa
    54241 atgtttttcc atttgcagta acttgtacct ttacctctga tttgtgaggt aagctatttt
    54301 gaccaggaat aaatatttcc caattaccag acccaatttt tcttagagga tgagattttc
    54361 tattccaatt attaaagtca ccaattagtg acaaactatc tgcatttgga gcccattccc
    54421 tatagaagaa tccatcattt gtctgatgaa atccatagta aagatgagca tttgcaaaag
    54481 aactaaaatc tttatagttt ttcaatgttt ttgatttcat atcctcataa tgtttcattc
    54541 gcttttttat atcagattca aatggttgta ggtatacatc aatatctaaa attttaaatc
    54601 ttttttgact tttcattgta tttctcctcc ataataaata ttacatttaa aaaataatat
    54661 ttatctcata ctatcttctc tgtataattg atatatatta tataatccta ataaaaagct
    54721 taagccacca atgattaatt tcataattta cctccctagt atttatactt atatatcgta
    54781 ttcatcaaaa tttaaaagtt tgtttaaaac tcttttaata atatcataat caaagccttt
    54841 ataggaaaga tgttgagaaa ttttttgata tattttcttt ttatcttctt tctttactct
    54901 ttcatacctt ttttttgcta gatacatagc attttcaaat tcagtgtcct tgtctaattc
    54961 agatactgct tcattgattg tacttctatt gataccttta ttatataaat tctgcttaat
    55021 tctgtttttg ccacatttat ttaggttaac atttgtgtta acaatttttt gagctaatac
    55081 actgtcattt acgagattat aacttttaag aaaatctatg accctctcaa ttatattttc
    55141 atcaaattct gatgatagtt tttcttttaa ttttttttca gattggtcag catgagataa
    55201 aatatttaat gctttatttt tagcttttat atacatttca tcatctaaaa taggttttaa
    55261 ttcttcttca tttatttcat tccctttttt taaattaaat gtataaacaa gttctttaaa
    55321 tatagcaata aaaaatttat catctacata aatattaact ctatcatcat ttcttttttg
    55381 ttgttctatt tttgtaatta tacccataag tcctcctttt ttatatgtaa ttatattaac
    55441 aaaagcaact aataaaattt aacaaaaa
//
LOCUS       DAMJWP010000007        55295 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.007, whole genome shotgun sequence.
ACCESSION   DAMJWP010000007 DAMJWP010000000
VERSION     DAMJWP010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 55295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55295
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.007"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            63..1940
                     /locus_tag="PC700_000374"
     CDS             63..1940
                     /locus_tag="PC700_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2579702.1"
                     /translation="MVLGSKSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTG
                     AYEGTINYNQDPEYLNVWELQGITINSKNNPKTLNGQDLEKMGLNLKDYNVTQECIIE
                     DITSRKDVNKYLRKTSAPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGI
                     ISTPLATTYNAPILLVEKNNVPNSVKSELKRLNPKDIIIIGDENAISKTTANQIKSTV
                     NASQTRLNGSNRYETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPI
                     ILTDKDSITDNTYKWLKSEDLQNAYFIGGPQMISTNVINKVNGITKDSVTNNRVYGAD
                     RHETNANVIKKFYTEDEIEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYH
                     KDNLEAKSANKVYKIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGS
                     APGNSSGGMIEKDYNLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLKPD
                     LFTSIHYNGSTNKQGHGVEVFYKLKDKNGGTTKTVATNILNRILEKFKLTNRGIKTRV
                     LPSDSTKDYLYVLRSNDMPAVLVECAFLDNENDMSLINSSAKVKEMGTQIGKGIEDSL
                     K"
     gene            2168..4081
                     /locus_tag="PC700_000375"
     CDS             2168..4081
                     /locus_tag="PC700_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2579703.1"
                     /translation="MEYLFEKSSLKEYGNEALDKYRELLDKTDNNSRCILLLVPNNNT
                     KIRYERALRLNYSEELKITTYISFVKKELVKYWPLIIEKCDEINTKVVSPTFISNSLS
                     EYLIVQKVKEKRIQEGYFEDITGTNKSIANSIMTNINKSAFNLIDFDFIGEKIYSSKK
                     NKDSIYRFSYTQMDEIISYYINSLLSNSILDNALSIYLYNQYLLNDDFYLKNLFTEVR
                     YIIVDSLESSSNAEVDFITEALDNTLQSYIYFDYSRDYSVFNNVDIDYINEKIISKIK
                     PKEENIKKKEIRIEDLYLLPANIELNQSSQLYNEMLDLISEKVISLIEENVSPRDIAI
                     ISPINNSILEHQIRDSLIEKNIDVFSTKKDKKAVDYPYGNALVVATCIFYGYLDFIKD
                     DEYLSFLETLLEINRIKAFKIFNETRHLSIDNIDEHSQYRDILQYIEEKKKSDIKIHE
                     FLTQFYIDKMLNLKEGKKNVGLCKKIIAESESFSESLSLLRMKKEKIFIEALKSTIND
                     YYSVVDIEELKSKDKIVITTPYSYISSNIDRSIQIWVDIGSNAWNMKIEKDISNLIVL
                     RKSFEEKKIYTNEMEEYYKKYYLYNTVYNLLLNAKKIYAFKSEYAINGYIQESILYSI
                     LLKISHKGDKNYD"
     gene            4074..6293
                     /locus_tag="PC700_000376"
     CDS             4074..6293
                     /locus_tag="PC700_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDF2579704.1"
                     /translation="MININYREDQIPIINYDNGTMAVPAVPGAGKTFIITNLVAKLLL
                     EQKHKGGKILILTYMNSAVNNFKGRIRKILEENKIDDTNSYEVMTIHSLAVKIIKEKP
                     EVVMLSEDFNIADDLQKSIILNECISRFRFEGGERAFRWFLKEQKDERWKEIALEAWE
                     RGFFEFAGNAISELKYKGISPDKLEEILSRGHKGMLKIILPIYKLYDRKLKQNGLLDY
                     DDILILAEKTLSLDEGLRKKFQTRYKYIFEDECQDSNEIQGNIIKIISSENKNLVRVG
                     DINQSITGTFSSSDPKFFKEFINSADFCYRMDMSNRSSKDILDLANTLVKYVTKEFRQ
                     EECRHALEDMQIKTVPNGMGYKENPNPEHYNINVKWYESWKIEIEQTARYVKGIKNKY
                     PDKSIGILVPFNEQVTQVAKELRENNLTFEELGPNSLNKRKVINNIAYIIDFMLHCDD
                     VEKLIVVLNKVFIDTDNEEEIKYFLEELKNYSTEEIIYNFEFSKLDNDLIEFKEESDI
                     YLSFKRGIDIIKQILEYPIIRLDLLVLFIGKKLNLEKEDKAVLDYISFYIKYLISENI
                     NMDLEDVYNLLFDVKNRVFNHIIDVVYEINGYEPEPGSITLCNYHKSKGMEWDCVFLL
                     GLVEYNFPDNINQKFQSDKWYLKEKYKNPMAIIKSEVEAILKGSISTDYAHKTKIDSI
                     NEKIRLLYVGITRAKEMLVLSGSAYRDESDIGNKRKEQKPCIYLSRLNQHIIEKRSN"
     gene            6402..7232
                     /locus_tag="PC700_000377"
     CDS             6402..7232
                     /locus_tag="PC700_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HDF2579705.1"
                     /translation="MNDKLKYFSYSQNSINTYKSCPLKFKYKYIDRINWKNDDVGSRE
                     YYETLKTGRDFHLICERYFSNIPLGIYFNEDDKNSKKFLKWMENIKKVVPIEKGKTYL
                     PEYEVRMTLNGDIIQAKYDLVVIGNNNIEIWDWKTESKKIDYKHVENRIQTIVYMFLA
                     KEVIPKVLKMDINVKDINMRYYQPEFDDLPITILYDEEKHEANRNKIQNYISMIKNTN
                     YEEHTYEDNLYNDIEKVYNKEERMCYRNKKHCKYCEFNKLCNGKEIDYSILEAEIYGT
                     "
     gene            7222..10689
                     /gene="addB"
                     /locus_tag="PC700_000378"
     CDS             7222..10689
                     /gene="addB"
                     /locus_tag="PC700_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HDF2579706.1"
                     /translation="MGLRFVLGRSGSGKSTYILDEIKKEAQKNETTSIILLVPEQYTF
                     EAENRVSKLFLGKEKDKYLRVRVLSFKTLSNIVFSQVGGLTDVNINSSGKAMMVYRAI
                     EDVSEELNVFSKSKSQSGFVSSITDMISEMKQYNISPEMLENISGELDNETLSLKLKD
                     ISKIYNSFEGKLHENYVDAQDMLTSLASKIELSSYLDGACVYIDEFTGFTPNQYNVIK
                     SILNKSKSVNISLTVDDINYIGYSKSDMFSRTKFTYSKLTQLCNEEGIKILPQVNLNT
                     GVIKRFEKVKELQHLERFYNAYPYKIYSNPTENIKIKEFNNLYSEVEEIAREIVHLVR
                     DKNVRYRDITIATRDLNRYDFLVHSIFNEYNIPNFIDKKREAKSNPIVILIISALEMK
                     NRRYGYETMFRYLKSGLIGIDNDDINLLENYVLANGIKGKKWFDEKWDYRITQSLSGQ
                     ESEFELELKEKINEIKNRVLEPIVILQEKLRGKNRVKEICRYIYEFLLDINMPETIEN
                     LIVNFKDKGELDVANQYSQVWDIVVDILDQMVELMGDEIISLEKFIKLITLGFDEYEL
                     GLVPPSIDQVLVSSVDRMKNPDTKYLYLVGTTDGVFPLITKDSGILSDNDRESLGNKG
                     IEVDIDSKTRSFEEQFLVYKALTSTSKNLTITYPISDHEGKTLRPSIIISRLKKIFPN
                     IENKSYLVEENKSTDKDILKKITVKSPTFNELINVIKNYDSDDYNTEEINSIWLDIYR
                     YYLKDEIYSSITKKVIKGLSYTNQVHKIEEKKIRSLYKSNSLSISRLEKYAECPFAYF
                     IQYGLKAKKRKEYSFTPPDLGTFIHNILDRFSKELLQDNLTWRDIDEKYIELKIGIIV
                     DEIILKIPGYILNSSERYKYLAYRLKNMLTTAITIISQQIKQGSFEPIDYEVKFGDNG
                     KYPPIKMVLENGQEVSLIGQIDRVDEFEEGENKYIRIIDYKSGNKSISLTEIYYGLQL
                     QLLVYLDAILESAKDEDININPAAILYCRINNPIAKFNEDKDDEEIQEAILKELRMKG
                     LVVKDSHIVKEMDKSLIDGERKNSLVIPVGLTKDGNVGKSTSAISYEDFKLLRKYVRH
                     AIKDLCEEMLSGEIRIAPYKHKDGTSCDFCDYSTICQFDSTIKDNKYKNLNNKSNEEI
                     IKMMKGDVN"
     gene            10689..14516
                     /gene="addA"
                     /locus_tag="PC700_000379"
     CDS             10689..14516
                     /gene="addA"
                     /locus_tag="PC700_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HDF2579707.1"
                     /translation="MSSPKWTKEQLEVIGSRECNLLVAAAAGSGKTAVLVERIIQMIT
                     SRENPIDIDKLLVVTFTNAAASEMRERIGDAIGKALDENPENKHLQNQLVLLNKSSIT
                     TIHSFCLDVIKSNFHRINLDPNFRIGDQTECAILKQEAIEEVFEDLYEERDEGFLNLV
                     ESYAERGGDKEVQDIILGIYSFAMASPEPKKWLIDSAERFNIDENFDFSQSIWARAIL
                     DTVKIEINGLCLNMERALKEVESIEELETFAEKLSVEYKKIANISQACNKSWDEAYKK
                     MASMSFENYVKGVKRISKDAPSYIKESKEKAKTIRDKTKKSLESIVSATFNKDNDSIR
                     EEIKYLYNIVKPISSVVLRFEEEYSNKKREKGIIDFNDIEHFALNILTDIDEKGNIVP
                     SDIAVGYRNKFYEIFIDEYQDSNLVQEVLLKAVANTETPNRFMVGDVKQSIYRFRQAK
                     PELFLQKYNNYNDKKGSSHRKIMLYKNFRSREEVVDAVNYIFENIMNENIGEIEYTEK
                     ERLNLGANFNVDTDEKSIIGGATEIHLIQKDNKLDDDIINDKDDRINNKENEIEEEEK
                     LDNIQLEARMVGNIIKDLMKVNEDGKIQKVYDKGIDGYRPVEFRDIVILLRATSAWAP
                     VFADELMNMDIPTYADVGVGYFDTIEIKTILSLLQIIDNPMQDIPLISVLKSPIFGFT
                     PEDLIDIRVQSKDKIFYEVLKSTAEYDGFTDSQNETESEFIPSEECINKSKDFLIKLK
                     EFKEKSMYMSTDEFIWYLYTRTGYYAYVGALPGGSQRQANLKVLFERAKQFEETSLKG
                     IFNFVNFIEKLKKSSSDMGSAKTLGENANVVRIMSIHKSKGLEFPVVICSAMGKNFNT
                     QDFKKSILYHHNLGYGPQFVDYERRISFPSIAKEALKSKINIENLSEEMRVLYVAFTR
                     AKEKLIITGSTRNIQDSIKRWSNGIESLDTISQYEILKGKNFLDWIMPCVLRHRDLSN
                     LLEEVGLDAVFNVEHNSKWYGKLWNKNDILVEKKSDEEKESIEEILEKIDVDSPDSDY
                     YSEIEKKLNYIYPYEFSTRKPATISVTEIKKIQNNYEEELINTIFEQKVILKKPLFIQ
                     NEEEREKISGTERGTIVHLVMEVLDLKNVSSVNDIKSQIRGFVSKGIITEKQASIVNP
                     YKIYKFFASNIGKRMLNAEIINREKSIYAQVNMKDIYIYEKLINNDDKKLYDNESVML
                     RGIVDAYFEEDNQIVLVDYKTDFVNEENINQIIEKYKKQLDLYADIIETLTGKSVKEK
                     CIYLFGVDEAVCY"
     gene            14562..15773
                     /locus_tag="PC700_000380"
     CDS             14562..15773
                     /locus_tag="PC700_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HDF2579708.1"
                     /translation="MRFIHTSDWHLGKSLEGHSRIEEQAKFCEEFIKIVESNEIDMVI
                     IAGDVYDTSNPPAQAEKLFYQTVSRLANNGQRCVLIISGNHDNPERLSAITPLAHEQG
                     ILIYGYPLSATIEAKYKGFEITYATQGCTKLNINGENIVIATLPYPSEKRLNEVFSSE
                     DEFEKQKNYSEKVGDIFRSLEENFRSDTINIAVSHIFVIGGESTESERPIQLGGSFLV
                     ERKDLPEKAQYTALGHLHKQQKASERLNAYYSGSPLQYSKDERAYTKGAYIVDIKAGE
                     KPIIEDVYFNNYKPIEVFKCNGIEEALDICEQNQDREIWSYFEINTDEIISQNEIKKM
                     KELLKDIIEIKPIITSCYEQESVDIKEKSMAELFREFYSFSKGVEPKGELMDLFLDII
                     SEEGESADETN"
     gene            15760..19287
                     /locus_tag="PC700_000381"
     CDS             15760..19287
                     /locus_tag="PC700_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2579709.1"
                     /translation="MRPIRLELTGLNSYIDKQVIDFEKLIERGLFGIFGTTGSGKSTI
                     LDAITIAMYGNISRNTKEYINSVCDKAIISYEFEIGSKNTRRRYIVDRTIARSKTGTK
                     TSNARLIEVLNDNIQNVLADKVVEVNEKVAQVVGLTANDFTRSVVLPQDKFNEFLRLS
                     RADRRDMLERIFNLEKYGRSLGEKVKKRKNIQLQNLKDLKSKLSQYDGITEEVYNNIN
                     QELIELKNLEKDKNNALDLAQKSYEESKTVYEEQLKLEKNELRKNELDLKNSEIKEKI
                     ALVENDSNAKKVDPYISSVQNLEKKIDEDSFVVSNLEKKLAILNQELEVTKNRYEKIN
                     KIKNEEVPKLSEEKIRLQQAIKLEEELVLLDRELKDLKESGINLNKTKVELEKVKQVS
                     ESRKDAVTKSIKEVEGKIDKVNISAELKQKIFLAYEYEKDYNNVLEEKNQKLNKLEEI
                     LKETENINLKVRYIDKDKNDVNRNLENLSLHLDVLLKKCPGKSADLLLKSEYVTELRS
                     KANNTKENEIKKSSIQDELKIILESKFNTEREVNLLNEKLENNRKNRDDLEKELEELK
                     YLNLASELRRELKENMPCPVCGSKHHENHNITKYDENISFVKEKLEKLEKEKISIRNN
                     IEELNAKVSGYLSIEKMKTKELEDVKGKLGGIPSSQLLKKLDEEQRKLALLKSNIQEW
                     EKEKESTENKVTLAKEEKNKIEKEELKIRESLNNYKKLTRDLNIEIEGLENKCKKLKQ
                     EYLGLKTITKVSNLLSKVKEIRENEKELELLSTGHSNLLKNRDSLDIKIRENESKLHE
                     IEIELIKARELYVEKKLSRDNKYKEVINITKGDLAKNLLHNVEENICKILEQEESSKK
                     KLEEQRLEYEKNIAEKHNIDGRLKTAKEQYKDQKSTLNKLLAENKFESIYAVKRALLD
                     TDAVKKLIEEILEHEEEQKLLSFKIKSSKEKLNGRSIKKEYFEQLKDEIYNLKVEIGN
                     ISKDIGANQNQLITLKDSLDKINDFNKQLKVVEHNVDLLEELDKCVQGNKFVEYVSTN
                     QLKYIALEASKRLDGITKGRYALEIDSTLNFVMRDNFNGGERRSIDTLSGGETFLTSL
                     SLALALSSQIQLKGSAPLEFFFLDEGFGSLDSELLEIVIESLERLHSNNLSVGIISHV
                     EELKNRVPVKLLVSSSEAGIGSKVKIEYS"
     gene            19418..20671
                     /locus_tag="PC700_000382"
     CDS             19418..20671
                     /locus_tag="PC700_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDF2579710.1"
                     /translation="MLESPNNLIQIIFLIVLLIGSAFFSASETALMSLSKIRIRYMQD
                     EGVKGAKLVSSLIENPNKLLSSILVGNNVVNIAATSISTSLFIGLMGEKGVALATAVM
                     TVLVLIFGEITPKTIAANNSEKVSLLVSKPIKAIIFILRPIVWIFNIITNIIFKLFGI
                     TNKGAKSFITEEELKTMVNVSHEEGVLEMEEREIINNVFEFGDMQAKNAMVQRIDMVA
                     IDMEDSYDEIIQVFKTEKLSRMPVYEETIDDIVGILNIKDIIFLSDEEIESFDIKNYM
                     REPFFTYEFKKITQLLEEMKLEKSQMAIVVDEYGGTSGLLTIEDLVEVIVGDIEDEYD
                     EEEDEIQVIKEDEYIVDGSTKIGDVNELIGVNLESEEFDSIGGFIIGHLSRLPEENEV
                     IEVDNIRFCIESIEKNRIKKIRIYT"
     gene            20820..21871
                     /gene="ytvI"
                     /locus_tag="PC700_000383"
                     /pseudo
     CDS             20820..21871
                     /gene="ytvI"
                     /locus_tag="PC700_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437952.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
     gene            21962..23188
                     /gene="pepT"
                     /locus_tag="PC700_000384"
     CDS             21962..23188
                     /gene="pepT"
                     /locus_tag="PC700_000384"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDF2579711.1"
                     /translation="MKQKVVERFLKYVSFDTTSNSQCENCPSSEGQRVLAKYIVEELK
                     TMGVDDVSLDENSYIMATLKGNTDGVDTIGFISHLDTIEDVSGKDIKPRIIENYDGKD
                     IVLNEALNVITYVKDSPELEEFKGDDLIVTDGTTLLGSDDKAGIAEIVTAIEYLINHP
                     EIKHGDIKIGFTPDEEIGRGADLFDVEKFGAKYAYTLDGGIVGELECENFNAANATIT
                     IHGRNVHPGSAKNKMVNAIHIAAEISEMFPADERPETTEGYEGFWHLNSIGGNVENVS
                     MAYIIRDHCREKFENRKSIMIENIEKINKKYDNRVELDLKDSYYNMKEKIEPVMFIVD
                     IAKEAMEELGIKPRLVPVRGGTDGARLSFNGLPCPNIFTGGLNFHGKNECIPVSSMEK
                     ATKLIVRIAEKYAERV"
     gene            23395..24447
                     /locus_tag="PC700_000385"
     CDS             23395..24447
                     /locus_tag="PC700_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp18"
                     /protein_id="HDF2579712.1"
                     /translation="MKIKSKKIIAFALSLGIMLPSCVYADTISKNQNAQVDMNNYLQG
                     RKSPKEEKLIGEDRFDTAIKISQSGWNNGSERVFLVNSNSLPDALASTPLASKLDAPI
                     LLTNKNSIPYNVTEEIKRLNPAEVILIGSEGAISSSVKESLEDMEVLVSRIGGADREE
                     TSLLLTRQLDDTGDLGVSKVAVVNGYNGLADATSISSPAASDNTAIIYTGKDSIRSEA
                     KNFITQNSTKTYIVGGEYSISKKLEGQLVNSERLAGTDRKDTNAKVLEKFYGKSSKVN
                     NMYFAKDGSGREADLVDGLAAGVLASKTKSPVVLASGSLSSAQKSFIKKVKADKFVQV
                     GGGKNSKPYAEALALQ"
     gene            complement(24783..25352)
                     /gene="yfcE"
                     /locus_tag="PC700_000386"
     CDS             complement(24783..25352)
                     /gene="yfcE"
                     /locus_tag="PC700_000386"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HDF2579713.1"
                     /translation="MKIGIMSDTHGSLLYFEKALNVLSDCDVLLHGGDVLYHGPRNDI
                     PEGYNPKKFIETLNKLENIVIVKGNCDADVDQMVIEHPIQSPYVMSQFGEIRIILNHG
                     YIESEEEIIDKAKKMGGDILVLGHTHVKKLYMDDNLIVINPGSTSIPKDGSHSVAIID
                     IIKTDDEDELELDINLIDINTGNIININD"
     gene            complement(25377..26177)
                     /locus_tag="PC700_000387"
     CDS             complement(25377..26177)
                     /locus_tag="PC700_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HDF2579714.1"
                     /translation="MKRIITINTNQLNKSLETKELLTRKLINAGFEVYSDIYPDTELI
                     ISIGGDGSFLRTVRDFDFPEIPIMGINTGHLGFFPDILPDKIDSFIEAYTKKDYIIQE
                     MSLLNAEVYTTTSGSNMLAVNEVVIRGDKSRTIHLNLSLDNKHIQNFSGDGMIISTST
                     GSTAYNYSAGGSIVDINLELMQITPLHPINTNAYRCFTSSIICSNESVIKIAPEYRFE
                     DSVLIVVDGVEHRFRQIENIKVSISDAKIKLLRMSNYEFWHRVSEKFL"
     gene            26312..27184
                     /locus_tag="PC700_000388"
     CDS             26312..27184
                     /locus_tag="PC700_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2579715.1"
                     /translation="MFSIRNITKKLGKFKLNNINLELKEGDIVGIIGPNGSGKTTLIK
                     IIMGIIDADEGEIELCNETIENSPISFKNNIGFVYDSLQFYPHLKVKEFRKIVSLFYK
                     NFDREKFDEYLNKFDIEENMHIENLSKGQSEKLMLSSALSHNAKLLILDEPTAGIDPI
                     VRTEIMQYLQDFVKNGSSSVIISTHNTDNLIKIADYLVFINRGNQIFTVKKELIEQEY
                     KIIRANKAELEAIKESIVGVKEYKYYNEALVKVGDSLKVKSLLIEIDKHKVKNPTIEE
                     LMYYYVNEVRGCSI"
     gene            27172..27858
                     /locus_tag="PC700_000389"
     CDS             27172..27858
                     /locus_tag="PC700_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HDF2579716.1"
                     /translation="MFNLIRKDLIIGISSDGIRNLKYILLFFVFYFFLNSISYYTVSI
                     VVSYLIFINTFECDYENDSRIFIRSMPVSIEDIVYSKYLLGVGLIISVTIIVSLLSKL
                     TSLVFFRNMVLNDVFFSVNIFLAILTILLPLIFKFGYGKMKVCGFIVSILIYFVYGSL
                     LKMISMIVYQVKHFNYSKVGGVYLSNYVTDIANTKYINLYSMTFLTIIIFIISMYFSI
                     KISKKNKFNY"
     gene            27910..28452
                     /locus_tag="PC700_000390"
     CDS             27910..28452
                     /locus_tag="PC700_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2579717.1"
                     /translation="MKKFIKVSFFILGIFTLFIAINKLVYRDMVSVKDYEINHLEEIV
                     FDFDSRRSRDIGTIVRFKISNNSKYSYKLNSAKMKFENCVENKKGMNKCSAYVNVDLF
                     NEKNSITESTLKYGIKPNNQGYVDFVIPKGLNFDYRYFNESGMSVEYKGEYIVNIPMA
                     KGLYLVVGKNNDTWNANIME"
     gene            28531..28998
                     /locus_tag="PC700_000391"
     CDS             28531..28998
                     /locus_tag="PC700_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HDF2579718.1"
                     /translation="MSISNVREYFKQFGKEDSILEFEQSSATVELAAEAAGVIPARIA
                     KTLSFKIGDDAILIVTAGDAKIDNKKYKAEFNCKAKMLTPEEVLEFTGHAIGGVCPFG
                     LKNSIKVYLDDSMKRFDTVFPACGSSNSAIELTCEEMEKFSKSEKWVDVCKNW"
     gene            29706..30842
                     /locus_tag="PC700_000392"
     CDS             29706..30842
                     /locus_tag="PC700_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HDF2579719.1"
                     /translation="MDLNSKKYQMLKELYVSFAENEVKPLATELDEEERFPYETVEKM
                     AKAGMMGIPYPKEYGGEGGDTVGYIMAVEELSRVCGTTGVILSAHTSLGSWPIYQYGN
                     EEQKQKFLKPLASGDKLGAFGLTEPNAGTDASGQQTTAVLDGDEYILNGSKIFITNAI
                     AGDIYVVMAMTDKSKGNKGISAFIVEKGTPGFSFGVKEKKMGIRGSATSELIFEDCRI
                     PKENLLGKEGQGFKIAMSTLDGGRIGIAAQALGLAQGALDETVKYVKERVQFGRPLSK
                     FQNTQFQLADMEVKVQAARHLVYQAAINKDLGKPYGVEAAMAKLFAAETAMEVTTKAV
                     QLHGGYGYTRDYPVERMMRDAKITEIYEGTSEVQRMVISGKLLK"
     gene            30901..31683
                     /locus_tag="PC700_000393"
     CDS             30901..31683
                     /locus_tag="PC700_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDF2579720.1"
                     /translation="MNIVVCIKQVPDTTEVKLDPNTGTLIRDGVPSIINPDDKAGLEE
                     AIKLKEEMGAHVTVITMGPPQADMALKEALAMGADRGILLTDRAFAGADTWATSSALA
                     GALKNIDFDIIIAGRQAIDGDTAQVGPQIAEHLNLPSITYAEEIKTEGEYVLVKRQFE
                     DCCHDLKVKMPCLITTLKDMNTPRYMKVGRIYDAFENDVVETWTVKDIEVDPSNLGLK
                     GSPTSVFKSFTKSVKPAGTIYNEDAKTSAGIIIDKLKEKYII"
     gene            31702..32712
                     /locus_tag="PC700_000394"
     CDS             31702..32712
                     /locus_tag="PC700_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDF2579721.1"
                     /translation="MGNVLVVIEQRENVIQTVSLELLGKATEIAKDYDTKVSALLLGS
                     KVEGLIDTLAHYGADEVIVVDDEALAVYTTEPYTKAAYEAIKAADPIVVLFGATSIGR
                     DLAPRVSARIHTGLTADCTGLAVAEDTKLLLMTRPAFGGNIMATIVCKDFRPQMSTVR
                     PGVMKKNEPDETKEAVINRFKVEFNDADKLVQVVQVIKEAKKQVKIEDAKILVSAGRG
                     MGGKENLDILYELAEIIGGEVSGSRATIDAGWLDKARQVGQTGKTVRPDLYIACGISG
                     AIQHIAGMEDAEFIVAINKNPEAPIFKYADVGIVGDVHKVLPELISQLSVAKEKGEVL
                     AN"
     gene            32731..33528
                     /locus_tag="PC700_000395"
     CDS             32731..33528
                     /locus_tag="PC700_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HDF2579722.1"
                     /translation="MSTSDVKVYENVAVEVDGNICTVKMNRPKALNAINSKTLEELYE
                     VFVDINNDETIDVVILTGEGKAFVAGADIAYMKDLDAVAAKDFSILGAKAFGEIENSK
                     KVVIAAVNGFALGGGCELAMACDIRIASAKAKFGQPEVTLGITPGYGGTQRLTRLVGM
                     AKAKELIFTGQVIKADEAEKIGLVNRVVEPDILIEEVEKLAKIIAKNAQLAVRYSKEA
                     IQLGAQTDINTGIDIESNLFGLCFSTKDQKEGMSAFVEKREANFIKG"
     gene            33626..34471
                     /locus_tag="PC700_000396"
     CDS             33626..34471
                     /locus_tag="PC700_000396"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HDF2579723.1"
                     /translation="MKLAVIGSGTMGSGIVQTFASCGHDVCLKSRTQGAIDKCLALLD
                     KNLTKLVTKGKMDEATKAEILSHVSSTTNYEDLKDMDLIIEASVEDMNIKKDVFKLLD
                     ELCKEDTILATNTSSLSITEIASSTKRPDKVIGMHFFNPVPMMKLVEVISGQLTSKVT
                     FDTVFELSKSINKVPVDVSESPGFVVNRILIPMINEAVGIYADGVASKEEIDEAMKLG
                     ANHPMGPLALGDLIGLDVVLAIMNVLYTEFGDTKYRPHPLLAKMVRANQLGRKTKIGF
                     YDYNK"
     gene            34558..35733
                     /locus_tag="PC700_000397"
     CDS             34558..35733
                     /locus_tag="PC700_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDF2579724.1"
                     /translation="MREVVIASAARTAVGSFGGAFKSVSAVELGVTAAKEAIKRANIT
                     PDMIDESLLGGVLTAGLGQNIARQIALGAGIPVEKPAMTINIVCGSGLRSVSMASQLI
                     ALGDADIMLVGGAENMSMSPYLVPSARYGARMGDAAFVDSMIKDGLSDIFNNYHMGIT
                     AENIAEQWNITREEQDELALASQNKAEKAQAEGKFDEEIVPVVIKGRKGDTVVDKDEY
                     IKPGTTMEKLAKLRPAFKKDGTVTAGNASGINDGAAMLVVMAKEKAEELGIEPLATIV
                     SYGTAGVDPKIMGYGPVPATKKALEAANMTIEDIDLVEANEAFAAQSVAVIRDLNIDM
                     NKVNVNGGAIAIGHPIGCSGARILTTLLYEMKRRDAKTGLATLCIGGGMGTTLIVKR"
     gene            35928..36830
                     /locus_tag="PC700_000398"
     CDS             35928..36830
                     /locus_tag="PC700_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDF2579725.1"
                     /translation="MFKKENQRYNLISYTNEEEMTLKEVLLDKLNFSVRSLSKMKKEK
                     SVLVNGVYKKPSLKVYSGDLIEVKIDEEKANFEPQDLNLQIIYDDFDIIMVNKPPFMV
                     VHPTKSHYDKTIANGISYYIDNQKENVKIRFVNRLDMNTSGLVIVAKNAYAHHTLSTA
                     MSENKVEKKYITVVDGIIKENEGTIDEPIYRPTEDSIKRIIDERGQSSVTHYKVIERL
                     ENATVLEVSLETGRTHQIRVHMAHIGHGIIGDELYGYVDEELINRQALHAYKLEFEQP
                     RTKEKLKFKADIPEDMKELISKLR"
     gene            36844..37848
                     /locus_tag="PC700_000399"
     CDS             36844..37848
                     /locus_tag="PC700_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HDF2579726.1"
                     /translation="MIIHKFIIHVLDKNSDVPILNDFEGKVNQEVDGFFQKAIKRIAK
                     DEDLRKGVFKDYNDNLIKNCCEQIIYDESTFLKNSKEIASYLFDVMKINAALESCDLA
                     ICLYTIKDEKSVAILKLDYKKLYTHSIEFVDDKFNIQMVSNEIGIPETLRQKQGALIS
                     LSGINDEFHLRLLDKDAEKEGSESKFVTEFLNAKKIDDDKYKTKVFKNTAENWITNAL
                     SNDIKQAEDVRSILNYTLKEKHEVDINDFVDNSIKDDELKDSFKEHMEEKGLDESFSI
                     DKKWVEKKLKKRSIKTDNGFDIKGNLTDFEDPMKYTVKQNQDGTIDIIIKNVTFYEEK
                     "
     gene            complement(37992..38219)
                     /gene="acpP"
                     /locus_tag="PC700_000400"
     CDS             complement(37992..38219)
                     /gene="acpP"
                     /locus_tag="PC700_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDF2579727.1"
                     /translation="MFEKIKEIIAEQLGVDNLDEITMEASLMDDLEADSLDAVEIIMA
                     LEDEFGIEIPDEEAENFKCIGDICKYIEENK"
     gene            38391..38816
                     /locus_tag="PC700_000401"
     CDS             38391..38816
                     /locus_tag="PC700_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2579728.1"
                     /translation="MKYVGLLLTSVGMFLLIAVNFYYNSITLDMQRIEDYVMETNLIL
                     EDVAEKESYVSNEKEDYISRLMHVKKGIENSKTSFLIERYKEYKIKSIESLIYTISEE
                     KKDYLDEVDRYNKLGEKEINKLINKNFLEVTYLSITTYI"
     gene            38864..39070
                     /locus_tag="PC700_000402"
     CDS             38864..39070
                     /locus_tag="PC700_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2579729.1"
                     /translation="MTKLEDNMERFFGGCEGFFGNKMLIIIVIVFLLLCTDILEDLLC
                     DDNIWIWVILIVLLLFNFDDGCCC"
     gene            39252..39431
                     /locus_tag="PC700_000403"
     CDS             39252..39431
                     /locus_tag="PC700_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2579730.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFGDSWAEFDIMAWIPF
                     LIVLLILCCCGGCLE"
     gene            39465..39665
                     /locus_tag="PC700_000404"
     CDS             39465..39665
                     /locus_tag="PC700_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2579731.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFEDCWRDIDIMSWIPF
                     LILLLITCSCGGFFDGEVGCGC"
     gene            39918..40934
                     /gene="ccpA"
                     /locus_tag="PC700_000405"
     CDS             39918..40934
                     /gene="ccpA"
                     /locus_tag="PC700_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator CcpA"
                     /protein_id="HDF2579732.1"
                     /translation="MKGNITIKDVAKQAGVSISTVSRVINDSKPVTDEVKQKVLEVIK
                     ETGYIPNPLARSLVTKKSQLIGVIVPEVSDSFVNEVLNGIEEVAKMYDYDILLANTYS
                     DKEQELKSINLLRAKQVEGIVMISWIVEQEHINYIQNCGIPATYISKTARNYDIYTVS
                     TSNEEATFDMTEHLIKKGHEKIAFIMTSKDDTVLEMERLAGYEKALSNNNIELDKSLI
                     KYGGTDYESGYNSMKELLDDGIIPHAAFVTGDEAAIGAINAICDAGYKVPEDISVAGF
                     NDVKIARMYRPKLTTVYQPLYDMGAVAIRMVIKLINKELIENKKIELPYRIVDRESVT
                     ERKK"
     gene            complement(41057..41455)
                     /locus_tag="PC700_000406"
     CDS             complement(41057..41455)
                     /locus_tag="PC700_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2579733.1"
                     /translation="MKKLDTESLEKMAKQKNIDKDKIEKMADSYKGKSENELMEELIK
                     IGKNLDGRDEVVSKFKAFLDENQRKKLDNIMEKISDAENQRDTKPAKTKKAKPTKGKS
                     NSPAPKNTPGGQQKKSKGLFKKTKKSNPHE"
     gene            41667..42572
                     /locus_tag="PC700_000407"
     CDS             41667..42572
                     /locus_tag="PC700_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2579734.1"
                     /translation="MNNLLFLIGVIALSNGNISIFDDKNKSKGNNRVRKNKKNANDKL
                     SQSTHGNLRFDANDIKRGLRLMDLSEEDLEMGMEIITRTKKYMSRDERKILIKIESVL
                     DLVRGIKKLNNIDMVDTEDETDFFRKMDSDDKKNMMIKEIIDVFPEKRKNSVEKAIGM
                     KKKIDLFAELFLPDDFGEGGFSLSSLANINNLGSMNNLKLLGSLLRGDDSNNDDEDNN
                     DELNYEDEELDCEDKELDDDELTDEGEMEDNEDELVEDKNTGYENIENDEDIVYDEEL
                     YNINEQESEYNYNEKNTKTRAKKKR"
     gene            42687..42867
                     /gene="ssrS"
                     /locus_tag="PC700_000408"
     ncRNA           42687..42867
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PC700_000408"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            43050..44267
                     /gene="cdeC"
                     /locus_tag="PC700_000409"
     CDS             43050..44267
                     /gene="cdeC"
                     /locus_tag="PC700_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeC"
                     /protein_id="HDF2579735.1"
                     /translation="MQDYKKNKRRMMNQPMSTMNEEEVYTDEINSEDMRGFKKSHHHN
                     GCNTDNKCECHDECNPCNPCNPCKPNPCNPCKPNPCDDKCECHDKCKCDCEPCEMDSD
                     ECFENKCGPECCNPISPRNFSVSNAVPFAIEANRIFDTMQFQTFTDATGPNGEPLTFE
                     TEVVEVFGSIPSAGQASVTIEKICLSNDGIVIDTGMTTLEDFDLDPLGDIVGRNCETT
                     FEFAVCGERNAECCRQGKGKSVAYKQRGLTVAVRNLVLELRGRCGCTEFVALAFPAVR
                     AGGGCKRRVDYVEFTFNTLSAPICLPADGRAVTLRQEYQTNLTVDCIGKSILKLECNE
                     CCEPFYELIIPNDIDLVLCLQETVSTLISEQIVVLASPNPIQPRLVDTFSKVCDFSQC
                     GPNHGSGKPSCHR"
     gene            complement(44368..45690)
                     /locus_tag="PC700_000410"
     CDS             complement(44368..45690)
                     /locus_tag="PC700_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDF2579736.1"
                     /translation="MKVPYLVLSTVILFISNFIVRIFGFLYKIFLSRALGETGLGIYH
                     MIFNFLMICLAVTTTGIPTALSCLVAKRKALNDRHNTNALFISTLYISFFVALIISIV
                     ASFNSSFLSLKFLKDAKLNLFILAVCPAIVIITLSNVLRGYYYGIKNVKIPAIGQIIE
                     QIGKILFVFLLVMYINNKSMNCYIALLGISIGELSNIIFMLICLWRDSSFDNRYIISI
                     KDFYNSSMETLKMSIPITCNRMSNILLQSISSMMIPSRLALSGMTYQQSLSMYGIVSG
                     MVMPFIFLPFTVGSALIVNLIPTISQEMALRKRKSVIKKIKYSILLTLFVGILSSIFF
                     YFFGKDLCILVFKNTLAGEYLKAMFLVPLFMSLNQTLSGILHSIRKELASSINTITGM
                     LIQLIALYVFLPIPGLNIYAYIYTMTIVSIFTCLLHTIVLFKSLKSIR"
     gene            complement(45741..46535)
                     /locus_tag="PC700_000411"
     CDS             complement(45741..46535)
                     /locus_tag="PC700_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HDF2579737.1"
                     /translation="MFYDYHMHSSFSTDGKSTMEEMVKKSIELGLEEICFTDHVDYDV
                     YADDSFSIVYEDYFKSLETLQSKYKDKISIKKGIEFGVQTQLMDTYKKEAHQYPLDFI
                     ICSIHAIDTMDLYLGNYFKDKTQYEVYENYYLYLYNIVKNYKDYSVLGHLDLIKRYAP
                     YDTILDDRLFSDIIEETLKQAIYDGKGIEINTSCYRYNLPDLTPSKYILQMYKDLGGE
                     IITTGSDSHHISQVACEFDYIYSLLKNMGFKYVSKFNKLKPEFIKL"
     gene            46645..48090
                     /locus_tag="PC700_000412"
     CDS             46645..48090
                     /locus_tag="PC700_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2579738.1"
                     /translation="MEKYRIDLNEKKSTKYIQIFNHIKELIINKELVEHEKLPPIRKL
                     SNFLQVNNTTIVKVYELLENEGYVYKIIGSGTFVSSSNSYKSGIDNRKNKIIVQREDL
                     IHFDNGNPSNDMFPIDSFKNAVNMALSKDGSSIFEYDEGLGSEELREKMVEYLSDGNI
                     KTTKENIQIISGAQQGIDIVCKGLISYSDVVFMEEPSYNGAIEVFKSRGAKIISIPML
                     DDGIDIGILKLKLEKIKPRLIYIMPNFQNPTGISYSTYKKKKLIELAEEHDFYIIEDD
                     FISDFVFESKDNRTIRSYDDKNRVVYIKSFSKILMPGLRIGIVEMPNELLKKVLWAKY
                     SSDISTPGLIQKSMYYYMNNFDWKNYLSHIEKIYTNKYKLSKNLINDKLSGKLKVRKS
                     CGGINFFLELPRGYTSQAFTNFMLNKGVSMLPGTYFFDNLVDDRFFRINIARPSMEEL
                     EKGISVISDNIDEFFCEYKNKLDIKSNKLFY"
     gene            48119..48328
                     /locus_tag="PC700_000413"
     CDS             48119..48328
                     /locus_tag="PC700_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HDF2579739.1"
                     /translation="MFDKKKLDRINELAKKNKEGILSADEIKEREILRKEYLENFRAH
                     FRSRLDSVKVVSPEEYEQYMKNNKN"
     gene            48348..49742
                     /locus_tag="PC700_000414"
     CDS             48348..49742
                     /locus_tag="PC700_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDF2579740.1"
                     /translation="MNLKYEFKNAWDFERKENTIEQIMQYSRNYMEFLSKSKTERLSV
                     KEIIKLAKENNYISIDEAMEKGSISCGDKIYVINKEKAVALFVIGKNYIEKGMKIIGS
                     HIDSPRLDLKPNPLYQESNLGFFKTHYYGGIKKYQWTALPLALHGIVILNDGTKVDIS
                     IGEEDSDPVFCVTDLLIHLAGDQMQKKLSEGISGEALNILIGNIPLEDEEKEPITANI
                     LKILNEKYNIVEEDLLSAEIEVVPAGKARDLGFDRSMVLGYGHDDRVCSYAAVKAILE
                     TEQPEFTSVALCVDKEEIGSKGNTGMHSKFFENTVAELIALEGDYCDIKVRRALANSK
                     VLSADVSAGYDPNFGEAYEKRNSAYMGNGVVLTKYTGSRGKSGCNDANAEFMSEVKRI
                     FNKGNVVWQTAELGKVDQGGGGTIAHILANQGAEVIDCGVGVLNMHAPHEIVSKVDIY
                     EMYKGYKAFFNINL"
     gene            49982..50734
                     /locus_tag="PC700_000415"
     CDS             49982..50734
                     /locus_tag="PC700_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2579741.1"
                     /translation="MDEKRQKKEMDLEVNSFVPLYQQLYDNIKKQIASGIYKPGDKLP
                     SEGDLCKEFNISRITVRNALNELVKEDILCKKRGKGTYVTIPERIEATCAGNSFTNSC
                     HRINAKPSTKIISVLIKKADKQVAEALSIELEEKVICIKRLRLIDEVPVIFEVDYFRI
                     DYMFLLKEELEDKSLMEVISNNISTLPKRVENIFEVKHSNKEYSDYLKCASNMPLLKV
                     RQSVYTENNDVLYYNEQFIRSDKYKYAVSAEI"
     gene            50760..51173
                     /locus_tag="PC700_000416"
     CDS             50760..51173
                     /locus_tag="PC700_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HDF2579742.1"
                     /translation="MKKIILASHGDFSKGLLNAVKMIVGDLADCVSSYGLYPGQSASD
                     FALELEKTISEDIECEYIILSDLYGASVCTAMLRLTNLHNVKLFSGMNLNMVLELLTR
                     FSDELTVEDIEQLVEESRRGIQSVTLQIKEEEEVF"
     gene            51177..51920
                     /locus_tag="PC700_000417"
     CDS             51177..51920
                     /locus_tag="PC700_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane biogenesis protein"
                     /protein_id="HDF2579743.1"
                     /translation="MKKKGILSVFKSKKPIIAMIHLKGDTPEDIFERAKKEITIFEEN
                     GVDGIMLENYYGNYYDLERILEYVSKANLSIPYGVNCLNVDTMGFELATKYNASYIQV
                     DSVVGHVKPRDEATLEEFFKLQRSKCPAYLIGGVRFKYQPVLSENDVEEDLKIGMTRC
                     DAIAVTENATGQETSMEKIELFRKNLGDFPLVIAAGVTLENAKKQLELGDMAIIGSYF
                     KDNYKDFGNVSVEHVKTFMDEIKKIREEL"
     gene            51920..52390
                     /locus_tag="PC700_000418"
     CDS             51920..52390
                     /locus_tag="PC700_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2579744.1"
                     /translation="MIKLVRVDHRLIHGQVAFTWTKFLSTDCILIASDELLKDELRMA
                     GLKMAKPSNVKLVMKSIADSIKALNSGVTDKYNLLILCESVEDVYRLAKEVKAIKSIN
                     LGGTKSDDNRENISKAVHVSKDDIKMIKELDSEGVNVFVQLVPDDDATNVMKLI"
     gene            52407..53165
                     /locus_tag="PC700_000419"
     CDS             52407..53165
                     /locus_tag="PC700_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2579745.1"
                     /translation="MEFTQVILITLIAFFAYMHSFVGSTMHNRPIVVAPLVGLALGNL
                     HTGIVIGSTLELVFMGAFPVGASNPPDFVSGSIIATAFVILTGQDVSAAVVLAVPIAT
                     LVLLIDNFLMTVVLTWGAHIADRYAEEGNIEGVERVQLLFGIGNKLILAIIVGIGFSL
                     GVPVIEKILSFIPSYVTHGMDVAAGVIPAIGFAMLARMMLNKKTVAFLLLGFILVAYL
                     NITVTGVALFGLAIALIYVNFVGEKEVIVDDNEF"
     gene            53149..53991
                     /locus_tag="PC700_000420"
     CDS             53149..53991
                     /locus_tag="PC700_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF2579746.1"
                     /translation="MTTSSKKLDTISPDSKITRKDFWKCFRRSLTLDSSWNYERMQNI
                     AYAYMMAPIIRRLYKDDKEKKSKALKRHLEFMSVTPHISTLLVGISGAMEEENAKNKE
                     FDANSINAVKSSLMGPVSGIGDSFFWGTLKLIAAGVGIALASQGNIMGPILFLLIINV
                     PHFIIRYICLDKGFKYGTQFFKDVSSSSIVSKVMEAASMLGLMVIGGMTASNVMLKLS
                     VNVGSGEWAEPIQTYLDQIMPCMLPAMIFGIMYWLLGKKVKTTTILISVMIICIVLAA
                     IGIV"
     gene            complement(54151..55056)
                     /locus_tag="PC700_000421"
     CDS             complement(54151..55056)
                     /locus_tag="PC700_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2579747.1"
                     /translation="MDFKQLEVFVAVAKHQSFSKAARELFLTQPTVSAHIQNLERELE
                     TVLINRSNKVITLTKSGEILYEHAIYILNNCKRAIYDIKEYSGKIEGIIDIACSSIPE
                     TYILPDFMKSFSMSYPDVKFSISHYDSQYAISEILNERISFGLVGSKINNPQIEYLDL
                     LDDELVLITPSDFKIDNKNNCIDIGELAYLNFIMRKEGSGTRNLILNTLSKNNFPVSK
                     LNVIAHVESNEAIKEMVRLGLGVSFISYISAIDYLNAGKIKCYKIKGVDFTRKFFFIY
                     SKKKTFSPLEDKFLNRLCEYFEIII"
BASE COUNT        22479 a         5765 c         9804 g        17247 t
ORIGIN      
        1 ctactgaatc taatgctatg gaaaataacg ctgatttaaa tataaactta gagaaaaaaa
       61 gtatcgtttt aggtagcaaa tcaaaagtta gtgtcaaatt taaagaaaaa ccagatgcag
      121 atagcattac attaaagtat aaatgctatg acatgccatt ggatacaact ctaaattaca
      181 atcaatcaac tggggcatat gaaggaacta tcaattataa ccaagaccca gaatatctaa
      241 atgtttggga actacaaggg ataacaataa acagcaaaaa taatcctaaa actttaaacg
      301 gacaagacct agaaaagatg ggattaaatt taaaagacta taatgtaaca caggaatgta
      361 taattgaaga cataacttct agaaaagatg taaataaata tttgagaaaa acttctgcac
      421 ctattacaga acttacagga agtgatagat atgaaacagc agttaaaata agtaaagagg
      481 gctggaaaaa tggttcagat aaagtagtta taataaatgg agatgtaagt atagatggca
      541 ttatatcaac tccgctggca accacatata atgctccaat acttttggtt gaaaaaaaca
      601 atgtgcctaa tagtgtaaaa tcagaattaa agcgcctaaa ccctaaagat ataattataa
      661 ttggagatga gaatgctatt tctaaaacta ctgctaatca aattaaatcg actgtaaatg
      721 ctagtcaaac acgtttaaat ggttctaata gatatgagac atctttattg atagcaaagg
      781 aaatagataa aaatcatgat gtggaaaaag tatacataac aaatgctaat ggcggagaag
      841 tggatgcact tactatagca gcaaaagcag gtcaagacaa gcaaccaatt atattaactg
      901 ataaagatag tattacagac aatacatata aatggttaaa gagtgaggat ttacaaaatg
      961 cttattttat aggtggtcct caaatgatat caacaaatgt tataaataag gtaaatggaa
     1021 taactaagga tagtgttact aataatagag tatacggagc agatagacac gaaacaaatg
     1081 caaatgtaat aaaaaaattc tatacagagg atgagataga ggctgtttta gtagctaaat
     1141 cagatgtact tgttgatgct ttagcagcag gtccattggc tgcgaactta aaatctccaa
     1201 tacttataac accaaagacg tatgtatctg cataccataa agataattta gaagctaaat
     1261 cagctaataa agtatacaaa ataggtggag gattgacttc taaggtaatg agctctatag
     1321 catcatcatt atctaaacac aatacgactc caacagaacc aggaaatagt gggggcaaga
     1381 cagttatgat tgacccaggg catggtggtt cagcacctgg aaattcatct ggaggaatga
     1441 ttgaaaaaga ttacaattta aatacttcac ttgcaacaac tgaatattta cgttcaaagg
     1501 gattcaatgt aataatgaca agagacacag ataagacttt atctcttgga aatagaactg
     1561 ctctatctaa ttcattgaaa ccagatttat ttacaagtat acattataat ggctcaacta
     1621 ataaacaagg tcatggtgta gaagtatttt ataagcttaa agataaaaat ggggggacta
     1681 ctaaaactgt agctaccaat atattaaata gaattttaga gaaatttaaa cttacaaata
     1741 gaggtataaa aacaagagta cttcctagtg attctacaaa agattattta tacgttttaa
     1801 gaagtaatga tatgccagct gtacttgtag aatgtgcatt tttggataat gaaaatgata
     1861 tgagtttaat aaactcatct gcaaaagtaa aagaaatggg tacacaaata ggtaaaggaa
     1921 tagaagattc attaaaataa atggaaataa aaatatttaa aaacttaatg taaatttata
     1981 ctaaagtcaa aaaatgaaat ataagtgtat caatagtaag actgtatgta catttgcaca
     2041 tgtgaatgca agtatatata cagtcttttt ttgtagttaa atgcaataat ttgattgtgt
     2101 tttgtaaaat acacaatatg atataattag ataatataca cttaatttaa ttgaggaagg
     2161 tgattatatg gaatatttat ttgaaaaaag tagtttaaaa gaatatggaa atgaagcttt
     2221 agataaatat agagaattat tagataaaac tgataataat tcaaggtgta ttcttttatt
     2281 agtaccaaac aataacacaa agatacgata tgaaagggct ctaagattaa attatagtga
     2341 agaactgaaa ataacaacat atataagttt tgtgaagaaa gaattagtta aatactggcc
     2401 actaataata gaaaaatgtg atgaaataaa tacaaaggtt gtatctccaa catttatatc
     2461 aaatagttta agtgaatatt taatagtgca aaaagttaag gaaaaaagga tacaagaagg
     2521 atattttgaa gatattacag gaaccaataa atccatagca aatagtataa tgactaacat
     2581 aaataaatct gcatttaatt taattgattt tgattttatt ggagaaaaaa tatattcatc
     2641 aaagaaaaat aaagatagta tatatagatt ttcatacact cagatggatg aaattatatc
     2701 atattatata aattcgttac tatcaaattc aattttagat aatgcattaa gcatatattt
     2761 atacaatcaa tacctactta atgatgattt ttatttaaaa aacttattta cagaagtgag
     2821 atatataatt gtggatagtc ttgaaagctc aagtaatgca gaagttgatt ttataacaga
     2881 agcattggat aatacattac aaagttatat atattttgat tatagtaggg attattctgt
     2941 atttaataat gtggatatag actatataaa tgagaaaata atatcaaaaa ttaagcctaa
     3001 agaagaaaat attaaaaaga aggagattag aatagaagat ttatacttat tacctgcaaa
     3061 tatagaatta aatcaatcaa gtcaattata caatgaaatg ttagatttaa taagtgaaaa
     3121 agttatatct cttatagaag aaaatgtatc tccaagagat atagcaatta tatctcctat
     3181 aaacaatagt atattggagc atcaaattag agattctttg atagaaaaaa atatagatgt
     3241 ttttagtact aagaaggata aaaaagctgt agattatcca tatgggaatg cattggtagt
     3301 ggctacatgt atattttatg gatatttaga ttttataaaa gatgatgaat acttaagctt
     3361 tttagaaact cttttagaaa taaataggat aaaagcattt aagattttta atgaaacaag
     3421 acatttaagt attgataata tagatgaaca tagtcagtat cgtgatattt tacagtatat
     3481 tgaagaaaaa aagaagagtg atataaaaat acatgaattt ttaactcaat tttacataga
     3541 taaaatgtta aatttaaaag aaggtaaaaa gaatgttggg ttatgtaaaa agataatagc
     3601 tgaaagtgag tcttttagtg aaagtttaag tctacttagg atgaaaaaag aaaagatatt
     3661 tatagaagct ttaaaaagta caataaacga ttattactct gttgtcgata tagaagagtt
     3721 gaaatccaaa gacaaaatag taataactac tccttattca tatatatcat caaatataga
     3781 tagaagtata caaatatggg tagatatagg aagtaatgct tggaatatga aaatagaaaa
     3841 agatataagt aatttaattg tacttagaaa atcttttgaa gaaaaaaaga tttatacaaa
     3901 tgaaatggaa gaatattata aaaaatatta tctatacaat acagtatata atttgttatt
     3961 aaatgcaaag aaaatctatg catttaaaag tgaatatgcg attaatggtt atatccaaga
     4021 gagtatatta tatagtatac ttttaaaaat ctcacataaa ggagataaaa attatgatta
     4081 atataaatta tagagaagac cagataccaa taataaatta cgataatggt actatggcag
     4141 taccagcagt accaggtgct ggaaaaacat ttataataac aaatttagta gctaaactat
     4201 tacttgaaca gaaacataag ggtggtaaaa tattaattct tacatatatg aatagtgctg
     4261 taaataattt taaaggtaga ataagaaaga ttcttgaaga gaataaaata gatgatacta
     4321 attcgtatga agtaatgact atacacagtt tggctgtaaa aataataaag gaaaagccag
     4381 aagttgtaat gttaagtgag gattttaaca tagctgacga tttgcaaaaa agtattatat
     4441 taaatgaatg tataagtaga tttagatttg aaggtggaga aagagctttt agatggtttt
     4501 taaaggagca aaaagatgaa cgctggaaag aaatagctct tgaagcctgg gaaagaggtt
     4561 tttttgaatt tgcaggaaat gcaataagtg aacttaagta taagggaatt tctccagata
     4621 aacttgaaga aatattgtca agaggacaca aaggaatgct taaaattatt ttaccaatat
     4681 ataaattgta tgatagaaaa ttaaagcaaa atggattgct agattatgat gatatattaa
     4741 ttttagcaga aaaaacttta agcttagacg aagggcttag aaagaagttt caaactagat
     4801 acaaatatat atttgaagat gagtgtcagg actcaaatga aattcaaggt aatataataa
     4861 aaattatatc tagtgaaaat aaaaacctag ttagagttgg agatataaat cagagtataa
     4921 caggaacatt ttcatcatca gaccctaaat tttttaaaga atttataaat agtgcagatt
     4981 tctgttatag gatggatatg tcaaatcgaa gttcaaaaga tatacttgat ttagcaaaca
     5041 ctcttgtcaa gtatgttaca aaggaattta gacaagaaga atgtagacat gctcttgaag
     5101 atatgcaaat aaaaactgtg ccaaatggaa tgggatataa agaaaatcct aatccagaac
     5161 attataatat aaatgtaaag tggtatgaga gttggaaaat tgaaatagag caaactgcta
     5221 gatacgttaa gggtataaaa aataaatatc cagataaaag tataggtata ttggttccat
     5281 ttaatgaaca agtgacacaa gttgctaaag aacttagaga gaataatcta acctttgaag
     5341 aattaggacc aaattctttg aataaaagga aggttataaa caacatagca tatattattg
     5401 attttatgtt acattgtgat gatgtagaaa agttaatagt tgtacttaat aaagttttta
     5461 tagatactga taatgaagaa gaaataaagt attttttaga agaattaaaa aactattcta
     5521 ccgaagaaat tatttataat tttgaattta gtaagttaga taatgattta atagaattta
     5581 aagaagaaag tgacatatat ttaagtttta aaagaggaat agatattata aaacagatat
     5641 tagaatatcc aataataagg ttagacctct tagttttgtt tattgggaaa aaacttaatt
     5701 tagaaaaaga agataaagct gtactggatt atatatcttt ttatataaag tatctaatat
     5761 cagaaaatat aaatatggat ttagaagatg tatataattt attgtttgat gtaaagaata
     5821 gggtgtttaa tcatataata gatgtggtat atgagataaa tggatatgaa ccagagcccg
     5881 gtagtataac actatgtaac tatcataagt caaaggggat ggaatgggat tgtgtgtttt
     5941 tactaggtct tgttgaatat aatttccctg acaatattaa tcaaaagttt cagagtgata
     6001 aatggtatct aaaagaaaaa tataaaaacc ctatggcaat tattaaatca gaagttgaag
     6061 ctatattaaa aggaagtatt tcaacagatt atgctcataa aacgaagatt gattctataa
     6121 atgaaaagat tagactttta tatgtaggga ttactagagc aaaggaaatg ttagtacttt
     6181 caggaagtgc ttatagagat gaaagtgata tagggaataa gagaaaagag caaaaaccat
     6241 gtatatattt aagcagactt aatcaacata ttattgaaaa aaggtcaaat taatatataa
     6301 taagttatta aaagattgct tgaaatatat ctaaaagggg ataaaacaat atgtacagta
     6361 agtaactact aaaaaataaa gttaagagag aagtgatatg agtgaacgat aaattaaaat
     6421 atttttctta tagtcaaaat tcaatcaaca catataaatc atgtccattg aaatttaaat
     6481 ataaatatat tgatagaata aattggaaaa atgatgatgt tggtagtaga gagtattatg
     6541 aaactttaaa aacaggaaga gattttcatt tgatttgtga aagatatttt agcaatatac
     6601 ctcttggaat ctattttaat gaggatgata aaaatagtaa aaagttttta aagtggatgg
     6661 aaaatataaa aaaagttgtt cctattgaaa aaggaaaaac gtatttgcca gaatatgaag
     6721 taaggatgac cttaaatggg gatataatac aggcaaagta tgatttagtt gtaattggaa
     6781 ataacaatat agaaatatgg gattggaaaa ccgagtctaa aaagattgac tataaacacg
     6841 tagaaaatag aatacaaact attgtttata tgtttttagc aaaagaagta atacctaaag
     6901 ttttaaaaat ggacataaat gttaaagata taaatatgag atattatcaa cctgaatttg
     6961 atgacttacc aattaccatt ttatatgatg aagaaaaaca tgaagcaaat agaaataaaa
     7021 ttcaaaatta tataagtatg ataaaaaaca ctaattatga agaacataca tacgaagaca
     7081 atctatacaa tgatatagaa aaggtatata ataaagaaga acgtatgtgc tatagaaata
     7141 aaaagcattg caagtattgt gaatttaata agttatgtaa tggcaaagaa atagattata
     7201 gtattttgga ggcggaaatt tatgggactt aggtttgtat taggtagaag tggaagtgga
     7261 aaatccacct atatactaga tgagattaaa aaagaagctc aaaaaaatga aacgacatca
     7321 attatacttt tagttccaga acagtacaca tttgaggctg aaaatagagt aagtaagcta
     7381 ttcttaggaa aagaaaaaga taagtatcta agagttaggg tacttagctt taagacacta
     7441 agtaatatag ttttttctca agtaggagga ttgacagatg taaatataaa ttctagtgga
     7501 aaagcaatga tggtatatag agctattgaa gatgtaagtg aagaattaaa tgtgttttca
     7561 aaatcaaaat cgcagtctgg ttttgtaagc tcaattacag atatgatatc agaaatgaaa
     7621 caatacaata taagccctga aatgttagaa aatatatctg gagaattgga taatgaaact
     7681 ttaagtttaa aattaaaaga tataagtaaa atatataatt cttttgaagg gaaattgcat
     7741 gaaaattatg tagatgctca agatatgtta acttcattgg caagtaaaat tgaattaagc
     7801 agttatttag atggagcttg tgtttatata gatgaattta caggttttac accaaatcag
     7861 tacaatgtca taaagagtat attaaataag tctaaaagtg ttaatatctc tttgactgta
     7921 gatgatatca actatattgg gtacagtaaa tctgatatgt tttctagaac gaaatttact
     7981 tattcaaaat tgactcaatt gtgcaacgaa gaaggcataa aaatattgcc acaagtaaat
     8041 ttaaacacag gtgttataaa aagatttgaa aaagttaagg aattacagca cctagaaaga
     8101 ttttataatg cttatccata taaaatatat tcaaatccaa ctgaaaatat aaagataaaa
     8161 gagtttaaca atttatattc agaggtggaa gagatagcca gagaaattgt acatttagtt
     8221 agagataaga atgtaagata tagagatata accattgcta caagagattt aaatagatat
     8281 gattttttag tacactctat atttaatgaa tataatatac caaactttat agataagaag
     8341 agagaggcaa aaagtaatcc aatagttatt ttaattattt cagctttaga aatgaagaat
     8401 agacgatatg ggtatgaaac tatgtttagg tatttaaaaa gtggacttat aggaattgat
     8461 aatgatgata taaatttact agaaaattat gtgttggcta atgggattaa aggtaaaaaa
     8521 tggtttgatg aaaaatggga ttatagaata acacaaagtc tttcaggtca agaaagtgaa
     8581 tttgaactgg aattaaaaga aaaaataaat gaaataaaaa atagagtatt agagcctata
     8641 gtaattttac aagaaaaatt aagaggaaaa aatagagtaa aagaaatctg tagatatata
     8701 tatgaatttt tattagatat aaatatgcca gaaactatag aaaatttaat agtaaatttt
     8761 aaggataaag gggaacttga tgttgcaaat caatactcac aagtttggga catagtagtg
     8821 gacatattag accaaatggt ggaacttatg ggtgatgaaa taatttcttt agaaaaattt
     8881 ataaaattaa taaccttagg ctttgatgaa tatgaattag gactggttcc tcctagtata
     8941 gaccaggtac ttgtaagtag tgtggataga atgaaaaatc cagacacgaa atatttatac
     9001 ctagttggaa ctactgatgg tgtgtttcca ctgattacaa aggatagtgg aatattgagt
     9061 gataatgata gggaaagtct tggaaacaaa ggaatcgaag ttgatattga tagtaagact
     9121 aggagttttg aagaacagtt tcttgtatat aaagctctta cttcgacatc taagaattta
     9181 accataacat atccaatatc agaccatgaa ggtaaaacgc ttagaccatc aataattata
     9241 tcaagactta agaagatatt tccaaatata gaaaataaaa gttatttagt ggaagaaaat
     9301 aaaagcactg ataaagatat acttaaaaaa ataacggtta aatcccctac atttaatgaa
     9361 cttataaacg taataaaaaa ttatgacagt gatgattaca atacagaaga gattaatagt
     9421 atttggttag atatatatag gtactattta aaagatgaaa tctatagttc tataacaaaa
     9481 aaagttataa aaggtcttag ttatactaat caagttcata agattgaaga gaaaaaaata
     9541 aggtcattgt ataaaagtaa ttctcttagc atatctaggc ttgaaaaata tgctgaatgt
     9601 ccatttgctt attttataca atatggtcta aaagcaaaaa agcgtaaaga gtattcattt
     9661 acaccaccag atttaggaac ttttattcat aatattttag ataggttttc aaaagaactt
     9721 ttacaagata atttaacttg gagagatatt gatgaaaaat atatagaatt aaagataggt
     9781 ataatagttg atgaaataat tttaaagata ccaggatata tattaaatag ttctgagagg
     9841 tataaatacc ttgcatatag attaaagaat atgttgacaa ctgctataac tataattagt
     9901 cagcaaataa aacaaggttc atttgaacca atagattatg aagttaaatt tggcgacaat
     9961 ggaaaatatc cacctataaa aatggtgtta gaaaatggac aagaagtaag tttaattgga
    10021 caaatagata gagttgatga atttgaagaa ggagaaaata aatatataag aataatagat
    10081 tataagtcag gcaataagtc cataagtcta acagaaatat attatggtct acaattacaa
    10141 ttacttgtgt atttagatgc aatccttgaa agtgcaaagg atgaagatat aaatataaat
    10201 ccagctgcaa tactatattg tagaataaat aatccaatag caaaatttaa tgaagataaa
    10261 gatgatgaag aaatacaaga agcaatatta aaagaactta gaatgaaggg gcttgtagta
    10321 aaagactctc acatagttaa agagatggat aaatcattaa tagatggaga aaggaaaaac
    10381 tctttagtaa taccagtagg tcttactaag gatggaaatg taggtaaaag tacttcagca
    10441 ataagctatg aggatttcaa actgcttaga aaatatgtaa ggcatgctat aaaggattta
    10501 tgtgaagaaa tgttaagtgg agaaatacgt atagctccat ataaacataa agatggaaca
    10561 tcatgtgact tttgtgatta ctctactata tgtcagtttg attcaactat aaaagataac
    10621 aagtataaaa atttaaataa taagagtaat gaagagataa taaaaatgat gaaaggagat
    10681 gttaattagt gagttctcct aaatggacaa aagagcaatt agaagttata ggaagtagag
    10741 aatgcaattt attggttgct gctgctgctg gttcaggaaa aacagccgtt ctagttgagc
    10801 gtataattca aatgataaca agtagagaaa atccaattga tatagataag ttattagttg
    10861 ttacatttac taatgctgct gcgtcagaaa tgagagagag gataggggat gctataggaa
    10921 aagccttaga tgaaaaccca gaaaataaac atttacaaaa ccaacttgtt ttattaaata
    10981 aatctagtat tacaacaata cactcattct gtttggatgt tataaaatca aactttcata
    11041 gaataaactt agaccctaat ttcagaatag gagaccaaac tgaatgtgct attttgaaac
    11101 aagaagcaat agaggaagta tttgaagatt tatatgaaga aagagatgag gggtttttaa
    11161 atttagttga aagttatgca gaaagaggag gagataaaga agtacaagat attatcttgg
    11221 gaatatattc atttgcaatg gcttctcctg aacctaaaaa atggttgatt gactcagcag
    11281 aacgatttaa tattgatgaa aattttgatt tttcacagtc tatttgggct agagctatat
    11341 tagacactgt aaaaatagaa attaatggtc tttgtctaaa tatggaaagg gcattaaaag
    11401 aagtggaatc aatagaagaa ttagaaactt ttgcagaaaa gttatccgtt gaatataaaa
    11461 aaattgctaa catttcacaa gcatgtaata aatcttggga tgaggcatat aaaaagatgg
    11521 caagtatgtc ctttgaaaat tatgtaaaag gagtaaaaag aatttcaaaa gatgctcctt
    11581 cgtatataaa agaatcaaaa gaaaaggcaa aaactataag agataagact aagaagtctt
    11641 tagaaagtat agtaagtgct acattcaata aagataatga ttctattaga gaagaaataa
    11701 aatatcttta taacatagtt aaaccaatat ctagtgttgt actgagattt gaagaggaat
    11761 atagcaataa gaaaagagaa aaaggaataa tagattttaa cgatatagag cattttgcat
    11821 taaatatact tacagatata gatgaaaaag gtaatattgt accttctgat atagccgttg
    11881 gatatagaaa caagttttat gaaatattta tagatgaata tcaggatagt aatctagtgc
    11941 aggaagtact gcttaaagct gttgcaaata cagaaactcc aaatagattt atggttggtg
    12001 atgtaaaaca gagtatatat agatttagac aagcaaagcc agaattattt ttgcaaaaat
    12061 ataataacta taatgataaa aagggaagtt cccatagaaa aataatgctt tacaaaaatt
    12121 ttagaagtag agaagaagtt gtagatgctg taaattatat atttgaaaat ataatgaatg
    12181 agaatatagg agaaattgaa tatacagaaa aagaaagact aaatttaggt gcgaatttta
    12241 atgtggatac agacgagaaa tctattattg gtggagcaac ggagatacac ctaatacaaa
    12301 aagataataa acttgatgat gatattataa atgataaaga cgatagaatt aataacaaag
    12361 aaaatgaaat agaggaagaa gaaaaattag ataatataca acttgaggct agaatggttg
    12421 gaaacattat caaagattta atgaaagtca atgaagatgg aaaaattcaa aaggtctatg
    12481 ataagggaat agatggatat agacctgtgg aatttagaga tatagttatt cttttaagag
    12541 ctacatctgc atgggctcca gtgtttgcag atgaattgat gaatatggat ataccaacat
    12601 atgctgatgt tggagtgggg tattttgata cgattgaaat aaaaacaata ttgtcattac
    12661 ttcaaataat agataatcca atgcaagata tacctcttat atctgtatta aaatcaccta
    12721 tatttggatt tacaccagag gatttgattg atattagagt tcagagtaaa gataaaattt
    12781 tttatgaggt tttaaaaagt acagcagagt atgatggatt tacagattct caaaatgaaa
    12841 ctgagtctga gtttatacca agtgaagagt gcataaacaa aagtaaagat tttttaatta
    12901 agctaaagga atttaaagaa aagtcaatgt acatgagtac agatgaattt atatggtact
    12961 tgtacacaag gactggatac tatgcttatg ttggagcatt gccaggtgga agtcaaagac
    13021 aagctaattt aaaagtattg tttgagagag caaaacaatt tgaagaaacc agtcttaaag
    13081 gaatattcaa ctttgtaaat tttatagaga aattaaaaaa atctagttct gatatgggaa
    13141 gtgcaaaaac acttggtgag aatgcaaatg ttgttagaat aatgagtatt cataaaagta
    13201 aaggtctaga atttccagta gtcatatgct ctgcaatggg taaaaatttc aatactcaag
    13261 attttaaaaa gagtatttta tatcatcata atttagggta tggacctcaa tttgtagact
    13321 atgagagaag gatttctttt ccaagtatag ccaaagaagc attaaaaagc aaaataaata
    13381 tagaaaattt atcagaagag atgagagttt tatatgttgc atttacaaga gctaaagaaa
    13441 aattgattat aactggttct actagaaata tacaagatag tataaagaga tggtcaaatg
    13501 gaattgaaag tttagataca atatcacaat acgagatatt aaaaggtaaa aattttttag
    13561 attggataat gccttgtgta ttaagacata gggatttatc taatctacta gaagaagtag
    13621 gattagacgc agtttttaat gttgaacata attcaaaatg gtatggaaag ttgtggaata
    13681 agaatgatat tttagtggaa aagaagagtg atgaagaaaa agaaagtatt gaagaaattc
    13741 ttgaaaaaat agatgtagat agtccggaca gtgattatta cagtgaaata gaaaaaaaat
    13801 taaactatat atatccatat gaatttagta caagaaaacc tgctactata tctgttacag
    13861 aaataaaaaa gatacaaaat aattatgaag aagagttaat aaatactata tttgaacaaa
    13921 aagtaatatt aaaaaagcct ctatttattc aaaatgaaga agaaagagaa aaaataagtg
    13981 gaacagaaag aggtacaata gtccatttgg ttatggaggt tttagactta aaaaatgtaa
    14041 gtagtgtaaa tgatataaaa tcacaaataa gaggttttgt ttcaaaaggt ataataacag
    14101 aaaaacaagc tagtatagta aatccatata aaatatataa attttttgcc tctaacatag
    14161 gaaaaagaat gttgaatgct gaaattataa atagagaaaa atcaatttat gcacaagtaa
    14221 atatgaaaga catatatatt tatgaaaagc ttataaacaa tgatgataaa aaactttatg
    14281 ataatgaaag tgtcatgtta agaggtatag ttgatgctta ttttgaagaa gataatcaaa
    14341 tagttttggt ggattataaa acagattttg taaatgagga aaatataaat caaataatag
    14401 agaaatataa aaaacagttg gatttatatg cagatattat tgaaacttta actggtaagt
    14461 cagtaaaaga gaaatgtata tatttatttg gagttgatga agctgtttgt tattagtttt
    14521 tatatataaa ttaaacctaa aagatagagg aaggggaaaa aatgagattt attcatacat
    14581 cagattggca tctagggaag tccctagaag gccattctag aattgaggaa caagcaaagt
    14641 tttgtgagga atttataaaa atagtagaat caaatgaaat agatatggtt ataattgctg
    14701 gagatgtata tgatacttct aatcccccag cacaagcaga aaaactattt tatcagactg
    14761 tatcaagact tgccaataat ggtcagaggt gtgtattaat aatttcagga aatcatgata
    14821 atccagagag actttctgca ataacacctc tagcacatga acaggggata cttatatatg
    14881 gttatccatt aagtgctacg atagaagcta agtataaagg gtttgaaata acatatgcaa
    14941 ctcagggatg tacaaaactg aatataaatg gagaaaatat tgtgattgct acattacctt
    15001 atccaagtga aaaaagatta aatgaagttt tttcaagtga agatgagttt gagaaacaaa
    15061 aaaattattc tgaaaaggta ggagatatat ttagaagttt agaagaaaat tttagaagtg
    15121 atactataaa tatagccgta tcacatatat ttgtaatagg tggagagagt actgaatcag
    15181 aaagacctat acaattaggt ggaagttttt tagttgaaag aaaagatttg ccagaaaagg
    15241 ctcaatatac tgcactaggt catttacata aacaacaaaa agcttccgaa cgtttaaatg
    15301 catactattc tggttctcca cttcaatata gcaaggatga aagagcgtat accaaagggg
    15361 catacatagt ggatataaaa gctggagaaa aaccaataat tgaagatgta tattttaata
    15421 attacaaacc tatagaagta tttaaatgta atggaataga agaagcacta gatatatgtg
    15481 agcaaaatca agatagagag atttggtcat attttgaaat aaatactgat gagataatat
    15541 cacagaatga aattaaaaaa atgaaggaac tattaaaaga cataatagaa ataaaaccaa
    15601 taataacatc ttgttatgaa caagaaagtg tagatataaa agaaaagtct atggcagagt
    15661 tgtttagaga gttctattct tttagtaaag gtgtagagcc aaaaggagaa cttatggatt
    15721 tatttctaga tataataagt gaagaaggtg aatcagcaga tgagaccaat tagattagaa
    15781 cttacaggac ttaatagcta tattgataaa caagttatag actttgaaaa attaattgaa
    15841 agaggtttgt ttgggatatt tggaactact ggaagtggaa aatctacaat cttagatgca
    15901 ataactattg ctatgtatgg aaatatatcc agaaatacaa aagaatatat aaacagtgtg
    15961 tgcgataagg caattatatc atacgagttt gagataggaa gcaaaaacac tagaagaaga
    16021 tacattgtag atagaaccat tgctagaagc aaaacaggaa caaagacctc taatgctaga
    16081 ttaatagaag tattgaatga caatatccaa aatgttttag ctgataaagt tgtggaagta
    16141 aatgaaaaag tagcacaggt tgtaggatta acggcaaatg attttactag gtcagtagtt
    16201 ttaccacaag acaaatttaa tgagtttcta agattatcac gtgcagatag aagagacatg
    16261 ttagagagaa tttttaatct tgagaaatat ggaagaagtc ttggagaaaa agtgaaaaag
    16321 agaaaaaata tacagttaca aaatttaaaa gatttgaaat caaaattaag tcaatatgat
    16381 ggaattacag aggaagtata caacaatata aaccaagaat taatagagtt aaaaaatttg
    16441 gaaaaagata agaataatgc tttagaccta gctcaaaaat cttatgaaga aagtaaaaca
    16501 gtatatgaag aacaattaaa gttagaaaaa aatgaactta gaaaaaatga gcttgattta
    16561 aaaaatagtg agataaaaga aaagattgct cttgtagaaa atgatagtaa cgctaaaaag
    16621 gtagacccat acataagttc agttcaaaat ctagaaaaaa agatagatga agatagcttt
    16681 gtagtaagta atttagaaaa gaaattagca atattaaatc aggagcttga agttacaaaa
    16741 aatagatatg aaaaaataaa caaaattaag aatgaagaag tccctaaatt gagtgaagaa
    16801 aaaataagat tgcaacaagc aataaaatta gaagaagagt tagttttatt agatagagag
    16861 ttaaaagatc taaaagaaag tggaattaat ttaaataaga ctaaagttga attagaaaaa
    16921 gtaaagcagg tgtcagagtc cagaaaagat gcagttacta aatctataaa agaagttgaa
    16981 ggaaagattg ataaagtaaa tataagtgca gaattaaaac aaaaaatatt tctagcatat
    17041 gaatacgaga aagattacaa caacgtattg gaagaaaaaa atcaaaagct aaataaacta
    17101 gaagaaatac tcaaagagac agaaaatata aatttaaaag ttagatatat tgataaagat
    17161 aagaatgatg taaatcgtaa tttagaaaat ttatcacttc acttggatgt tcttcttaaa
    17221 aaatgtcctg gtaagagtgc tgatttactt ttaaaatctg aatatgttac tgaattaaga
    17281 agtaaagcaa ataatactaa agaaaatgaa attaagaaaa gcagtataca agatgaatta
    17341 aagataatac ttgaaagtaa gtttaatact gaaagggaag taaatttact aaatgaaaaa
    17401 cttgaaaata atagaaagaa tagagatgac ctagaaaaag aattagaaga actaaagtat
    17461 ttaaatttag cctctgaact tagacgtgaa ttaaaagaaa atatgccttg tccagtttgt
    17521 gggtcaaaac atcatgaaaa tcataatata actaagtatg atgaaaatat atcttttgtc
    17581 aaagaaaaac tggagaaatt agaaaaagaa aaaataagta taagaaacaa tatagaggaa
    17641 ttaaatgcta aagtaagtgg gtatttatct atagaaaaaa tgaagactaa agaacttgaa
    17701 gatgtaaaag gaaaactagg ggggattcca tctagtcaat tattaaaaaa acttgatgaa
    17761 gaacaaagaa aacttgcatt gttaaaatct aatattcagg agtgggagaa agaaaaagag
    17821 agtactgaaa ataaagtaac attagctaaa gaagaaaaaa ataaaattga aaaagaagaa
    17881 ttaaaaataa gagagagtct gaataattat aagaaattaa caagagattt aaacatagaa
    17941 atagaaggtt tagaaaataa atgtaaaaaa cttaaacagg aatacctagg attaaagacg
    18001 attactaaag tctctaatct attgtcaaaa gtaaaagaaa taagggagaa tgaaaaagaa
    18061 ttagaattac taagtactgg gcattcaaat ttattaaaaa atagagactc actagatatt
    18121 aaaataagag aaaatgagag taaactacat gaaatagaga tagaattaat aaaggctaga
    18181 gaattatatg tagaaaagaa attgagtaga gataataagt ataaagaagt tattaacata
    18241 acgaaaggtg atttagctaa aaatctttta cataatgttg aagaaaatat atgtaagata
    18301 ttagaacaag aagaatcaag taagaaaaaa ttggaagagc aaagattaga atatgaaaaa
    18361 aacattgctg aaaagcacaa tatagatggt agacttaaaa ctgctaagga acaatataaa
    18421 gaccaaaaat caacactaaa taaactatta gcagaaaata aatttgaaag tatatatgca
    18481 gtaaaaagag ctttattaga tactgatgca gtaaaaaaat tgattgaaga aatactagag
    18541 catgaagaag aacaaaagtt attatcattt aagataaaat catcaaaaga aaaattaaat
    18601 ggaagaagca taaagaagga gtactttgaa cagttaaaag atgaaattta taatttgaaa
    18661 gtagaaatag gaaatatatc caaagatatt ggagctaatc aaaatcagtt aataacacta
    18721 aaagactctt tagataagat aaatgatttt aataaacaac ttaaggtagt tgaacacaat
    18781 gtggacttat tggaagaatt agataaatgt gtgcaaggaa ataaatttgt agaatatgtt
    18841 tctacaaacc aattaaaata tatagcattg gaagcatcaa aacgattgga tggaataaca
    18901 aaaggaagat atgcacttga aattgactca acactaaact ttgtaatgag agataacttt
    18961 aatggaggag aaagaagaag cattgatact ctatctggtg gagagacatt cttgacatct
    19021 ctatcattag cacttgctct ttcatctcaa atacaattaa agggaagtgc accattagaa
    19081 tttttctttt tagatgaagg ttttggttca ttagatagtg aacttttaga aattgttata
    19141 gaatccttgg aaagattgca tagtaataat ctaagtgtgg gtataataag tcatgtcgag
    19201 gaacttaaaa atagagttcc tgttaagcta ttagtttcat ctagtgaggc tggaataggt
    19261 tcaaaagtaa aaattgaata tagttagaaa atatatggta attgaatata ttatctaata
    19321 tttccaaata atatgtaatt gtgatataat aaaaactaat attaaagtta tttataatta
    19381 atattaatat aaaaacatgt aagtaggagt gtggtaagtg ttggaatcgc ccaataattt
    19441 gattcagatt atttttttaa tagtattact tatagggtct gcatttttct cagcatctga
    19501 aacggcctta atgtcgttga gtaaaatcag aatcagatat atgcaagatg aaggagttaa
    19561 aggagctaag ttagtaagtt cattaataga aaatccaaat aagcttttaa gttctatatt
    19621 ggttggaaat aacgtcgtaa atatagcagc aacatccata tcaacatctt tgtttatagg
    19681 attaatggga gaaaaaggag tggcacttgc tacagctgtt atgacagtat tagtattaat
    19741 atttggtgag attactccaa aaacaatagc agcaaataat tcagaaaagg tttctctatt
    19801 agtatctaaa cctataaaag caattatatt tatattaaga ccaatagtat ggatatttaa
    19861 tataattaca aatattatat ttaaattatt tggtataaca aataaaggag ctaaatcatt
    19921 cataactgaa gaagagctaa aaactatggt aaatgtaagt catgaagaag gcgtccttga
    19981 aatggaagag agagaaatca taaataatgt ttttgaattt ggagatatgc aagctaaaaa
    20041 tgcaatggta caaagaatag atatggtagc tatagatatg gaagatagtt atgatgagat
    20101 aatacaggta tttaaaactg aaaaattaag tagaatgcct gtgtatgaag aaactataga
    20161 tgatattgta ggaatactta atataaaaga tattatattt ttatctgatg aagaaataga
    20221 gtcatttgat attaaaaatt atatgagaga accttttttc acgtatgagt tcaaaaagat
    20281 aacacaactt cttgaagaga tgaaacttga aaaaagtcag atggctatag ttgtagatga
    20341 gtatggtgga acatctggac ttttaactat agaagattta gtagaagtca ttgttggaga
    20401 tatagaggat gaatatgatg aagaagagga tgaaatacaa gttataaaag aagatgagta
    20461 tattgtagat ggaagtacta aaattggtga tgttaatgaa cttattggtg taaacttgga
    20521 atctgaggag tttgattcca taggaggttt tataattgga cacctaagca gattgccaga
    20581 agaaaatgaa gtaattgaag ttgataatat aagattttgt atagaaagta tagaaaaaaa
    20641 tagaattaaa aaaataagaa tatatacata atgtaataat gttcttaaat ttattagaag
    20701 tgtataaata taaaagatat cttttatatt tatacacttc tttttatttt gtattcaaat
    20761 aaaactagta tgaaatggac aaaataaaaa tataaattag aagaaaggag atttttaaga
    20821 tgagcatact agaaaataat tttttataca aattaaaaaa caacactatc tttttttgta
    20881 tatatacatt gttatttatt tttatttaca aagcgtttcc atacatagct ccattttttc
    20941 taggtgctat aatagcactt atgattaatc ctatatcaca aaagttagaa aataagtttc
    21001 gtataaataa aggtatctct actcttgttc taagttttct tgcagtggct atagtcagta
    21061 cagttactac aataatagta ataaattcaa tgaaagaatt aatgggattt ttaaataata
    21121 tatctgctaa tcctgaggat ataagtaata ctattatgta ttttttaaat aaaataaatg
    21181 actttatgaa atcatttcaa gaaatagcca attttgatgt agaacaatta gtaaacaaat
    21241 tcagtggtga agtaatgcaa attacaaaaa atcttcttac tagtatactt ggattggcta
    21301 cttctattcc atatattata atatttataa ttaccttgtt tattgctacc tactttatag
    21361 caaaagacct tgataagatt gaaaatagtt tttataatat gtttactgtt gatgttagaa
    21421 agaaggtaaa aaacgtaaag aaagaggcag ggctttcact tgttggatat ataagggcct
    21481 atacaatact tatggctata acattttttg caatctgggg aagttttgct ttatttggat
    21541 tgaaatacgg tctaatagta gggtttgtag ggctttaatg gatttaattc cattcctagg
    21601 tataataaca atatatcttc cagtaattgt ttattatttt cttataaaaa attattttat
    21661 agctataagt atgactgtga tattttttgt tttgtctttg attagagaaa tacttgaacc
    21721 taaactagta tcagtaaatg ttggactaaa tccattagct accctagcag cgatttttat
    21781 tggaatccaa gtaaaaggaa taattggagt aattttttgt ttgggtttgg tatgtatgca
    21841 tgatatactt aagaaggtgg atatttttta attataataa ttgatagatt tttttaaaaa
    21901 taaaatttta ttcacaggta aataaaaaaa tattaatctt attaagaaag agggtatata
    21961 cgtgaaacaa aaagtagtgg aaagattttt aaaatatgta tcatttgata caacatctaa
    22021 ttctcagtgt gagaactgtc catcttcaga aggtcaaaga gttttagcta agtatatagt
    22081 tgaagagcta aaaacaatgg gtgttgatga tgtgagttta gatgaaaata gttatataat
    22141 ggctacttta aaaggaaata ctgatggtgt ggatactata ggattcatat cacatttaga
    22201 tacaattgaa gatgttagtg gaaaagatat taaaccaaga ataatagaaa attatgacgg
    22261 aaaagacata gttttaaatg aagcgttaaa tgttattacg tatgtaaagg attctccaga
    22321 gttggaagaa tttaaaggtg acgatttaat agtcactgat ggaacaactc tacttggctc
    22381 tgatgataaa gctggtatag ctgagatagt gactgcaata gagtatctaa taaatcatcc
    22441 agaaattaag catggagata taaaaatagg atttacacca gatgaagaaa ttggtagagg
    22501 tgctgattta tttgatgttg aaaaatttgg agcaaaatat gcatatactt tagatggagg
    22561 aatagttgga gaacttgaat gtgaaaattt taatgctgca aatgcaacta ttactataca
    22621 tggtagaaat gttcatccag gttcagctaa aaataaaatg gtaaatgcta tacatatagc
    22681 tgctgaaata tcagaaatgt tcccagcaga tgaaagacca gaaacaacag aaggatatga
    22741 aggattttgg catttaaatt ctataggtgg aaatgtcgag aatgtaagta tggcttatat
    22801 tattagagac cattgtagag aaaaatttga aaatagaaaa tctattatga tagagaatat
    22861 agaaaaaata aataagaaat acgataatag agtggaattg gatttaaaag attcttatta
    22921 caatatgaaa gaaaagattg agccagttat gtttatagtt gatatagcta aagaagctat
    22981 ggaggaatta ggtataaaac cacgtttagt tcctgtaaga ggtggtactg atggagcaag
    23041 attatctttt aatggattac cttgtccaaa tatatttact ggtggattaa acttccatgg
    23101 gaaaaatgag tgtataccag ttagttctat ggagaaagct actaaactaa ttgtaagaat
    23161 agctgaaaaa tatgctgaaa gagtttagtt aaaaagtatt taaaaattaa gcatttaaag
    23221 ttataatagc tatatcattc tttataagtc aagctgatta taagcttgtg ttgtatttaa
    23281 aatggtatag ctatttttgt aattattttt ataaaagata attattttta gtttagggta
    23341 tcaataaaaa gtatggatat caataggtat taacatttaa aagggggaat tgtattgaaa
    23401 attaaatcta aaaaaataat agcatttgca ctttcacttg ggattatgtt gccaagttgt
    23461 gtatatgcag acactattag taaaaatcaa aatgcacagg tagatatgaa caattatctt
    23521 caaggtagaa aatctccaaa ggaggaaaaa cttattggtg aagatagatt tgatacagcg
    23581 ataaaaatat ctcaaagtgg ttggaataat ggttcagaaa gggtgtttct agttaactca
    23641 aattcattgc cagatgcatt ggcttcaact cctttggcaa gtaaattgga tgcaccaata
    23701 ctgcttacaa ataaaaatag tataccatat aatgtaacag aggaaataaa aaggcttaat
    23761 ccagcagaag ttattttaat tggaagtgaa ggagctattt catcaagtgt aaaagaatct
    23821 cttgaagata tggaagtatt agtcagtaga atcggtggag cagatagaga agaaacatct
    23881 ttacttctta ctagacagct agatgatact ggtgatttag gtgtctcaaa agtggcagtg
    23941 gttaatgggt ataatggact ggcagatgca acttcaatat caagcccagc tgcaagtgat
    24001 aatacagcta taatatatac aggtaaggat agcataagaa gtgaggcaaa gaattttatt
    24061 acacaaaact caactaagac atacatagta ggtggggagt acagtataag taaaaaactt
    24121 gaaggacagt tagtaaactc agaaagacta gcaggaacag atagaaaaga tactaatgcg
    24181 aaagttttag aaaagtttta tggaaaatca agtaaggtta ataatatgta ttttgcaaaa
    24241 gatggaagtg gtagagaagc tgatttagtt gatgggcttg ctgctggagt attagcatca
    24301 aaaacaaagt cacctgtagt acttgcaagt ggaagtttaa gtagtgcaca gaaaagtttt
    24361 ataaaaaaag taaaggctga taaatttgta caagttggtg gaggtaaaaa tagtaaacca
    24421 tatgcagaag cattagcctt acagtaatat tgtgtaaatc aaaattaata tataataata
    24481 gcaagtaaac caagttgaac ttcaatatat ataataaaat aagtaaacta agttatattt
    24541 cagtataata tataatgaaa taagtaaact aagttatact ccaatataat atataataga
    24601 aacaagtaaa ctaagttata tttcaatata gcatataata aaacaagtaa accagattct
    24661 atttaattag aagtttaagg tattgtactt aaacttctaa ttgagataga aataaaatgg
    24721 tttacttgtt tttattatga gtttaataaa ttaaagctta ttttattttt attaaaaatt
    24781 tactaatcat tgatatttat tatatttcca gtattgatgt caattaagtt tatatcaagt
    24841 tcaagctcat cctcatcatc agtttttatg atatctataa ttgcgacaga atggcttcca
    24901 tcttttggga tagaagtact tcctggattt attactatta aattatcatc catgtatagt
    24961 ttttttacat gagtatgtcc taaaactaat atgtcaccac ccattttctt tgctttgtca
    25021 ataatttctt cttctgattc tatataacca tgattcaata taattctaat ttcaccaaat
    25081 tggctcatga cataaggact ctgaatagga tgttctataa ccatctggtc aacatctgca
    25141 tcacagttcc cttttacaat cacaatgttt tctaatttat ttaatgtctc aataaatttt
    25201 ttaggattat atccttctgg aatatcattt ctaggtccat gatataagac atctccacca
    25261 tgtaataaaa catcacaatc agataataca tttaacgctt tttcaaagta aagtaagcta
    25321 ccatgagtat cactcattat tcctattttc atattaaaaa tcctcctaag tacagtctat
    25381 aaaaattttt cagatactct atgccaaaac tcatagtttg acattctaag taattttatt
    25441 tttgcatcag atatactaac ttttatattt tcgatttgtc taaatctgtg ctcaactcca
    25501 tcaacgacaa tcaatacaga atcttcaaat ctatattctg gtgcaatctt tataacagat
    25561 tcatttgaac atattatact tgatgtaaag cacctatatg cattggtatt tatcggatgt
    25621 aaaggtgtaa tctgcataag ttcaagattt atatcaacaa tactacctcc agcagaatag
    25681 ttataggctg ttgaacctgt ggaagtagaa ataatcatac catctccact aaaattttgt
    25741 atatgtttat tatcaaggct taaatttagg tgtatagtcc tagacttgtc tcctcttata
    25801 acaacctcat ttacagcaag catattagaa ccactagtag ttgtatatac ttccgcatta
    25861 agaagtgaca tttcttgaat aatataatct ttttttgtat aagcttcaat aaagctatca
    25921 atcttatctg gtaaaatatc aggaaaaaac cccagatgac ctgtatttat tcccattatg
    25981 ggaatctcag gaaaatcgaa atctctaact gttcttaaaa aagagccatc accaccaatt
    26041 gatataataa gctctgtgtc tggataaata tctgaataca cttcaaagcc agcattgata
    26101 agttttctag ttaagagttc tttagtctca agggacttat ttaattggtt tgtatttata
    26161 gttataattc ttttcatata ttcacctctt catactttgt attataacat agttttataa
    26221 ttcttaattt ataaatttat aaatgacctt tgtagtgaat gtgttaaaat atgtataggt
    26281 gggtataaaa aaatgcataa gaggtgaaga tgtgtttagc ataaggaata tcacaaaaaa
    26341 attaggaaaa ttcaagttaa ataatataaa cttagagtta aaggaagggg atattgttgg
    26401 gattatagga ccaaatggtt cgggaaaaac gactttaata aaaattataa tggggattat
    26461 tgatgcagat gaaggtgaaa ttgaactttg taatgaaact attgaaaatt ctccaataag
    26521 ctttaaaaac aatattggtt ttgtatatga ttctttacaa ttttatccac atttaaaggt
    26581 taaggaattt agaaaaatag tttctttatt ttataagaat tttgatagag aaaaatttga
    26641 tgaatacctt aataagtttg acatagaaga aaatatgcat attgaaaatt tatctaaagg
    26701 tcaatctgag aagcttatgt tatcaagcgc acttagtcat aatgctaagt tattgatttt
    26761 ggatgaacca actgctggta tagacccgat tgtaagaact gaaataatgc agtacttaca
    26821 agattttgtg aaaaatggaa gtagtagtgt aataatttct actcataata ctgataattt
    26881 aattaaaatt gctgattatc ttgtttttat aaatagagga aatcagattt ttactgttaa
    26941 gaaagaatta atagaacaag aatataagat aattagggca aataaagcag agttagaagc
    27001 aattaaagaa agtatagttg gtgttaaaga gtataaatat tataatgagg cacttgttaa
    27061 agttggagat agcttgaaag taaaaagcct attgatagaa attgataagc ataaggtcaa
    27121 aaatcctact atagaagaac taatgtatta ttatgtaaat gaggtgagag gatgttcaat
    27181 ttaataagaa aagatttaat aataggaatt agttcagatg gtatccgtaa tttaaagtac
    27241 atattgctat tttttgtgtt ttattttttc cttaattcaa tttcatatta tacagtaagt
    27301 attgttgtta gttatttgat atttataaat acatttgagt gtgattatga aaatgattca
    27361 aggattttta taaggagtat gccagttagt atagaagata tagtttactc aaaatattta
    27421 ttaggtgtag gactaataat ttctgtaaca ataattgtgt ccttactaag taaattgaca
    27481 tcactagtgt tctttagaaa tatggtttta aatgatgtat ttttttctgt aaatatattt
    27541 ttagcaattt tgacgatttt actgccttta atttttaagt ttggatatgg aaaaatgaaa
    27601 gtttgtggtt ttatagtaag tattttaatt tactttgtat atggaagtct tttgaagatg
    27661 attagtatga ttgtatatca agtaaagcat tttaattata gtaaagttgg aggagtatat
    27721 ctttcaaatt atgtaactga tatagctaat actaagtata taaatttata tagtatgaca
    27781 tttttaacaa taataatttt tataatatca atgtattttt ctattaagat ttcaaaaaag
    27841 aataagttta attattaaaa ttttaattaa tattatagca caatagcaca aaatataagg
    27901 agactaaaaa tgaagaagtt tataaaagtt tcatttttta ttttggggat ttttacacta
    27961 ttcatagcta taaataaatt agtatataga gatatggtaa gtgtcaaaga ttatgaaata
    28021 aatcatttag aggaaattgt ttttgatttt gatagcagga gaagtagaga tatcggtact
    28081 attgtaagat ttaaaataag caataatagt aaatatagtt ataaattaaa cagtgctaaa
    28141 atgaagtttg aaaattgtgt ggaaaataaa aagggaatga ataagtgttc tgcatatgta
    28201 aatgtagatt tatttaatga aaaaaattct ataactgaaa gtactctgaa atatggaata
    28261 aaaccaaata atcaaggtta tgttgatttt gtaattccaa aaggactaaa ttttgattat
    28321 aggtacttta atgaatctgg tatgagtgta gaatataaag gagaatatat tgtcaatatt
    28381 ccaatggcaa aaggacttta tctagtagtt ggaaaaaata atgatacatg gaatgctaat
    28441 attatggaat aatttgaaaa ataaatcaat tatagatata attaagaata atatgaaatt
    28501 tataaaaatt cagactagga ggggtattta atgtcaataa gtaatgtaag agagtatttt
    28561 aaacaatttg gcaaggaaga cagtatatta gaatttgaac aatctagtgc tactgtggaa
    28621 cttgcagctg aagcagctgg ggttattcca gcaagaatag ctaagacact ctcatttaaa
    28681 ataggagatg atgcaatttt aattgtgaca gctggtgatg ctaagattga taataagaag
    28741 tataaggctg aatttaattg taaggctaaa atgttgacac cagaagaagt cttggagttt
    28801 actggacatg ctattggtgg agtctgtcca tttggtttaa aaaattctat aaaagtctat
    28861 ttagatgatt caatgaagag atttgataca gtatttcctg catgtggcag tagtaactct
    28921 gcaatagaac ttacatgtga agaaatggag aagttttcta agagtgaaaa atgggtagat
    28981 gtttgtaaga attggtaaaa aaatatagta aactatagta ggaattagta aaaaactacc
    29041 tctgtataca atctatacag aggtagtttt ttgtgtaaat atttataaat ctaagaggaa
    29101 aatctaattt aaactaataa tccaaaataa agttaaaaaa ttctgatttt ttttcgaaac
    29161 aataaaaaaa tgacaaaaaa gtggaaaaaa tgtaataaat attgttaaaa aaataattag
    29221 aaattattga aaaattatcc tttgagtgtt actattatag atgtaagata tttaaaaata
    29281 aaaaatagtc agataaaaat acgattattt agctctatta aaataatttt tatacgataa
    29341 aaatcttctg tacatggcta aaaaacagat gtacagacaa tatttaatgt ttaatatacc
    29401 ctaatataaa tatcgtaaac aaattatttt atctgtgacc agtgtgaaag aaatcttatt
    29461 ctaacttgaa atttcacaaa attccaaaaa gcgagatttt ttttaagtat agttgatttt
    29521 aaattaacaa tttatgatta aattaatgtc ataattggtt agtataatca catataaata
    29581 tagagcgtag tttgattaag gttataaatt tctcgacgta tattcaaata ccttattata
    29641 gatgttgccg agattttaat taataaatga aaataaaata tttataaaat actgaggagg
    29701 ctattatgga tttaaattct aaaaaatatc agatgcttaa agagctatat gtaagcttcg
    29761 ctgaaaatga agttaaacct ttagcaacag aacttgatga agaagaaaga tttccttatg
    29821 aaacagtgga aaaaatggca aaagcaggaa tgatgggtat accatatcca aaagaatatg
    29881 gtggagaagg tggagacact gtaggatata taatggcagt tgaagaattg tctagagttt
    29941 gtggtactac aggagttata ttatcagctc atacatctct tggctcatgg cctatatatc
    30001 aatatggtaa tgaagaacaa aaacaaaaat tcttaaaacc actagcaagt ggagataaat
    30061 taggagcatt tggtcttact gagcctaatg ctggtacaga tgcgtctggc caacaaacaa
    30121 ctgctgtttt agacggggat gaatacatac ttaatggctc aaaaatattt ataacaaatg
    30181 caatagctgg tgacatatat gtagtaatgg caatgactga taaatctaag gggaacaaag
    30241 gaatatcagc atttatagtt gaaaaaggaa ctcctgggtt tagctttgga gttaaagaaa
    30301 agaaaatggg tataagaggt tcagctacta gtgaattaat atttgaggat tgcagaatac
    30361 ctaaagaaaa tttacttgga aaagaaggtc aaggatttaa gatagcaatg tctactcttg
    30421 atggtggtag aattggtata gcagcacaag ctttaggttt agcacaaggt gctcttgatg
    30481 aaactgttaa atatgtaaaa gaaagagtac aatttggtag accattatca aaattccaaa
    30541 atacccaatt ccaattagct gatatggaag ttaaagtaca agcggctaga caccttgtat
    30601 atcaagcagc tataaataaa gacttaggaa aaccttatgg agtagaagca gcaatggcaa
    30661 aattatttgc agctgaaaca gctatggaag ttactacgaa agctgtacaa cttcatggag
    30721 gatatggata cactcgtgac tatccagtag aaagaatgat gagagatgct aagataactg
    30781 aaatatatga aggaactagt gaagttcaaa gaatggttat ttcaggaaaa ttattaaaat
    30841 agataaatat taaaatatac acaataggtc gatattaaaa tagatatgga ggaaggattt
    30901 atgaatatag tcgtttgtat aaaacaagtt ccagatacaa cagaagttaa actagatcct
    30961 aatacaggta ctttaattag agatggagta ccaagtataa taaaccctga tgataaagca
    31021 ggtttagaag aagctataaa attaaaagaa gaaatgggtg ctcatgtaac tgttataaca
    31081 atgggacctc ctcaagcaga tatggcttta aaagaagctt tagcaatggg tgcagataga
    31141 ggtatattat taacagatag agcatttgca ggtgctgata cttgggcaac ttcatcagca
    31201 ttagcaggag cattaaaaaa tatagatttt gatattataa tagctggaag acaggcgata
    31261 gatggagata ctgcacaagt tggacctcaa atagctgaac atttaaatct tccatcaata
    31321 acatatgctg aagaaataaa aactgaaggt gaatatgtat tagtaaaaag acaatttgaa
    31381 gattgttgcc atgacttaaa agttaaaatg ccatgcctta taacaactct taaagatatg
    31441 aacacaccaa gatacatgaa agttggaaga atatatgatg ctttcgaaaa tgatgtagta
    31501 gaaacatgga ctgtaaaaga tatagaagtt gacccttcta atttaggtct taaaggttct
    31561 ccaactagtg tatttaaatc atttacaaaa tcagttaaac cagctggtac aatatacaat
    31621 gaagatgcga aaacatcagc tggaattatc atagataaat taaaagagaa gtatatcata
    31681 taatttaagg ggggataaag aatgggtaac gttttagtag taatagaaca aagagaaaat
    31741 gtaattcaaa ctgtttcttt agaattacta ggaaaggcta cagaaatagc aaaagattat
    31801 gatacaaaag tttctgcatt acttttaggt agtaaggtag aaggtttaat agatacatta
    31861 gcacactatg gtgcagatga ggtaatagta gtagatgatg aagctttagc agtgtataca
    31921 actgaaccat atacaaaagc agcttatgaa gcaataaaag cagctgaccc tatagttgta
    31981 ttatttggtg caacttcaat aggtagagat ttagcgccta gagtttctgc tagaatacat
    32041 acaggtctta ctgctgactg tacaggtctt gcagtagctg aagatacaaa attattatta
    32101 atgacaagac ctgcctttgg tggaaatata atggcaacaa tagtttgtaa agatttcaga
    32161 cctcaaatgt ctacagttag accaggggtt atgaagaaaa atgaacctga tgaaactaaa
    32221 gaagctgtaa ttaaccgttt caaggtagaa tttaatgatg ctgataaatt agttcaagtt
    32281 gtacaagtaa taaaagaagc taaaaaacaa gttaaaatag aagatgctaa gatattagtt
    32341 tctgctggac gtggaatggg tggaaaagaa aacttagaca tactttatga attagctgaa
    32401 attataggtg gagaagtttc tggttctcgt gccactatag atgcaggttg gttagataaa
    32461 gcaagacaag ttggtcaaac tggtaaaact gtaagaccag acctttatat agcatgtggt
    32521 atatctggag caatacaaca tatagctggt atggaagatg ctgagtttat agttgctata
    32581 aataaaaatc cagaagctcc aatatttaaa tatgctgatg ttggtatagt tggagatgtt
    32641 cataaagtgc ttccagaact tatcagtcag ttaagtgttg caaaagaaaa aggtgaagtt
    32701 ttagctaact aatttcttgg ggagggtatt atgagtacaa gtgatgttaa agtttatgag
    32761 aatgtagctg ttgaagtaga tggaaatata tgtacagtga aaatgaatag acctaaagcc
    32821 cttaatgcaa taaattcaaa gactttagaa gaactttatg aagtatttgt agatattaat
    32881 aatgatgaaa ctattgatgt tgtaatattg acaggggaag gaaaggcatt tgtagctgga
    32941 gcagatattg catacatgaa agatttagat gctgtagctg ctaaagattt tagtatctta
    33001 ggagcaaaag cttttggaga aatagaaaat agtaaaaaag tagtgatagc tgctgtaaac
    33061 ggatttgctt taggtggagg atgtgaactt gcaatggcat gtgatataag aattgcatct
    33121 gctaaagcta aatttggtca gccagaagta actcttggaa taactccagg atatggagga
    33181 actcaaagac ttacaagatt ggttggaatg gcaaaagcaa aagaattaat ctttacaggt
    33241 caagttataa aagctgatga agctgaaaaa atagggctag taaatagagt cgttgagcca
    33301 gacattttaa tagaagaagt tgagaaatta gctaagataa tagctaaaaa tgctcagctt
    33361 gcagttagat actctaaaga agcaatacaa cttggtgctc aaactgatat aaatactgga
    33421 atagatatag aatctaattt atttggtctt tgtttttcaa ctaaagacca aaaagaagga
    33481 atgtcagctt tcgttgaaaa gagagaagct aactttataa aagggtaaaa atctaattaa
    33541 aaatatattt gctgttaact agattaaata gtattttaat ctagttaaca gaatataaat
    33601 actaaaataa caggaggtta taactatgaa attagctgta ataggtagtg gaactatggg
    33661 aagtggtatt gtacaaactt ttgcaagttg tggacatgat gtatgtttaa agagtagaac
    33721 tcaaggtgct atagataaat gtttagcttt attagataaa aatttaacta agttagttac
    33781 taagggaaaa atggatgaag ctacaaaagc agaaatatta agtcatgtta gttcaactac
    33841 taattatgaa gatttaaaag atatggattt aataatagaa gcatctgtag aagacatgaa
    33901 tataaagaaa gatgttttca agttactaga tgaattatgt aaagaagata ctatcttggc
    33961 aacaaatact tcatcattat ctataacaga aatagcttct tctactaaga gaccagataa
    34021 agttatagga atgcatttct ttaatccagt tcctatgatg aaattagttg aagttataag
    34081 tggtcagtta acatcaaaag ttacttttga tacagtattt gaattatcta agagtatcaa
    34141 taaagtacca gtagatgtat ctgaatctcc tggatttgta gtaaatagaa tacttatacc
    34201 tatgataaat gaagctgttg gtatatatgc agatggtgtt gcaagtaaag aagaaataga
    34261 tgaagctatg aaattaggag caaaccatcc aatgggacca ctagcattag gtgatttaat
    34321 cggattagat gttgttttag ctataatgaa cgttttatat actgaatttg gagatactaa
    34381 atatagacct catccacttt tagctaaaat ggttagagct aatcaattag gaagaaaaac
    34441 taagatagga ttctatgatt ataataaata agaatttggg aattaaaagt ttaaataaaa
    34501 ttgtttaaaa aacaatttcg gatatatgaa aaatctaatt taatgggggt aatgaatatg
    34561 agagaagtag taattgccag tgcagctaga acagcagtag gaagttttgg aggagcattt
    34621 aaatcagttt cagcggtaga gttaggggta acagcagcta aagaagctat aaaaagagct
    34681 aacataactc cagatatgat agatgaatct cttttagggg gagtacttac agcaggtctt
    34741 ggacaaaata tagcaagaca aatagcatta ggagcaggaa taccagtaga aaaaccagct
    34801 atgactataa atatagtttg tggttctgga ttaagatctg tttcaatggc atctcaactt
    34861 atagcattag gtgatgctga tataatgtta gttggtggag ctgaaaatat gagtatgtct
    34921 ccttatttag taccaagtgc gagatatggt gcaagaatgg gtgatgctgc ttttgttgat
    34981 tcaatgataa aagatggatt atcagacata tttaataact atcacatggg tattactgct
    35041 gaaaacatag cagagcaatg gaatataact agagaagaac aagatgaatt agctcttgca
    35101 agtcaaaata aagctgaaaa agctcaagct gaaggaaaat ttgatgaaga aatagttcct
    35161 gttgttataa aaggaagaaa aggtgacact gtagtagata aagatgaata tattaagcct
    35221 ggcactacaa tggaaaaact tgctaagtta agacctgcat ttaaaaaaga tggaacagtt
    35281 actgctggta atgcatcagg aataaatgat ggtgctgcta tgctagtagt aatggctaaa
    35341 gaaaaagctg aagaactagg aatagagcct cttgcaacta tagtttctta tggaacagct
    35401 ggtgttgacc ctaaaataat gggatatgga ccagttccag caactaaaaa agctttagaa
    35461 gctgctaata tgactattga agatatagat ttagttgaag ctaatgaggc atttgctgcc
    35521 caatctgtag ctgtaataag agacttaaat atagatatga ataaagttaa tgttaatggt
    35581 ggagcaatag ctataggaca tccaatagga tgctcaggag caagaatact tactacactt
    35641 ttatatgaaa tgaagagaag agatgctaaa actggtcttg ctacactttg tataggtggt
    35701 ggaatgggaa ctactttaat agttaagaga tagttttaga ttataatatt tacaaattaa
    35761 atatttaatt atgaatttaa cctgtatgaa taactatttg ttattcttac aggtttttat
    35821 agttaaaaat aattattatc tattaaacaa aaatagtgta aaatatataa tatcatgatt
    35881 ttgaagatat aaaaagcagg aagatataaa agaaagtagg taaaagtttg tttaaaaaag
    35941 agaatcaaag atataatctt atatcgtata caaatgaaga agaaatgact ttaaaagaag
    36001 ttttattgga caagctaaat ttctcagtta ggtcattatc aaaaatgaag aaggaaaaga
    36061 gtgttttagt aaatggagta tataaaaaac caagtttaaa ggtgtatagt ggtgatttaa
    36121 tagaagtaaa aatagacgag gaaaaagcaa attttgaacc tcaagattta aatttacaga
    36181 taatttatga tgattttgat ataattatgg tgaataaacc tccatttatg gtagttcacc
    36241 caacaaaaag ccactatgat aaaacaatag caaatggtat tagttactat atagataatc
    36301 aaaaagaaaa cgtaaaaata agatttgtaa ataggttaga tatgaatact tcagggttag
    36361 ttatagtagc aaaaaatgct tatgcacatc atactttatc tacagcaatg agtgaaaata
    36421 aagttgaaaa aaagtatata acagtagttg atggaattat aaaagaaaat gagggaacta
    36481 tagatgagcc aatttataga cctacagaag attcaataaa gaggataata gatgaaagag
    36541 gacagtcatc tgttactcac tataaagtaa tagaaagact agaaaatgca actgtattag
    36601 aagtaagctt agaaactggt agaacacatc aaataagagt acatatggct catataggtc
    36661 atggaataat aggtgatgag ttgtatggtt atgtagatga agagctaata aataggcaag
    36721 cacttcatgc atacaagcta gagtttgaac aaccaagaac aaaagaaaaa ttgaaattta
    36781 aagcagatat accagaggat atgaaagagt taatatcgaa attaagataa tggggtgata
    36841 tatatgataa tacataaatt tataatacat gttttggaca aaaatagtga tgttccaata
    36901 ttaaatgact ttgaaggcaa agttaatcaa gaagtagatg gttttttcca gaaagctata
    36961 aaaagaattg caaaagatga ggatttaaga aaaggtgttt ttaaagatta taatgacaat
    37021 ttaattaaaa actgttgtga acaaataata tatgatgaaa gtactttttt gaaaaattct
    37081 aaagaaattg catcttactt gtttgacgtt atgaaaatta atgctgcact agaatcttgt
    37141 gatttggcaa tttgcttata cactattaaa gatgaaaaga gtgtagcaat actgaaatta
    37201 gattataaaa agttgtacac tcattcaatt gaatttgtgg atgataaatt taatattcaa
    37261 atggtttcaa atgaaattgg tatcccagag actttaagac aaaagcaagg agctttgatt
    37321 agtttaagtg gtataaatga tgagtttcat ctaaggcttt tagataagga tgctgaaaaa
    37381 gaagggtcag aatctaagtt tgtaacagaa tttttaaatg ctaaaaaaat agatgatgat
    37441 aagtacaaga ccaaggtatt taaaaataca gctgaaaatt ggataactaa tgctcttagt
    37501 aatgatataa agcaagctga agatgtaaga agtatattaa attatacact gaaggaaaaa
    37561 catgaagttg atataaatga ctttgttgat aattcaatta aagatgatga gttaaaggat
    37621 agctttaaag agcatatgga agaaaaaggt cttgatgaaa gttttagtat agataaaaaa
    37681 tgggttgaga aaaagcttaa aaaaagaagt ataaaaacgg ataatggttt tgacataaaa
    37741 gggaatctaa ctgattttga agacccaatg aagtatactg taaagcaaaa tcaagatgga
    37801 actatagata taataataaa gaatgttaca ttttatgaag aaaagtaatt tatatactta
    37861 gtatataaat taaatataag agcgtgtatt ttatatattg taaataaatg taatctagtc
    37921 tgtgcgaact ctaatataat ttattatgta gaagtttaca cagactattt atatttgtag
    37981 acaaaacgat actatttgtt ttcttcgata tatttacata tatcaccaat acatttaaag
    38041 ttctcagctt cctcgtctgg tatttctatt ccaaattcat cttcaagggc cattataatt
    38101 tcaacagcat ctaatgaatc agcttctaag tcgtccatta aagatgcttc catagttatc
    38161 tcatctaaat tgtctacccc taattgttct gctattattt cttttatttt ctcaaacatt
    38221 ttaagcctcc taaagcagtt ttaattaatt ttaatgttag tttaatataa tatcaagaat
    38281 ttttcaatat ggaatctata aaaaataata aaattttata aatgtagtaa taaagtatta
    38341 tttgatgaat aaactttatt gataacaaat ataacttagg aggaaatgct atgaaatatg
    38401 taggactatt actaacttca gtaggaatgt ttttacttat agcagtaaat ttttattata
    38461 attcaataac tttagatatg caacgtatag aggattatgt aatggagacc aatttaatcc
    38521 ttgaagatgt agctgaaaag gagagttatg tatctaatga aaaagaagac tacatatcaa
    38581 ggttaatgca tgtaaagaaa ggaatagaaa attcaaaaac atccttcttg atagaaagat
    38641 ataaggagta caaaataaaa tcaatagaaa gtttaatcta cactatatca gaggagaaaa
    38701 aagattatct ggatgaagtt gatagataca ataaacttgg tgaaaaagaa ataaataaac
    38761 taatcaataa aaacttttta gaagtaactt atctgtctat tactacatat atttaattaa
    38821 agagacaaaa cttagtctct taatgattca aggaggatta gttatgacaa aattagaaga
    38881 caatatggaa cgtttctttg gaggatgcga aggatttttt ggtaataaaa tgttgataat
    38941 aatagttata gtattcttac tattatgcac agacatttta gaagacttat tatgcgatga
    39001 caatatatgg atatgggtta tattaatagt attgttatta tttaacttcg atgatggttg
    39061 ttgttgctaa aaaatattga ttattaaata ggtaaaacca gtaacttata caagttatgg
    39121 ttttaccttt tttagttcaa attaaaaaat ttaaaaaaat agtaaaaata cattagcaaa
    39181 atggaaacca aatgcatagt atatgttagg agcgaaaagc tcaacaaaat atcaacagga
    39241 ggtaacgtac aatgttagat agattctttg gagaagaagg atgttttggt ggaggaggat
    39301 ggtggataat agtactattc ttcctattct tagcttttgg agatagctgg gcagaatttg
    39361 acataatggc atggatacca ttcctaatag ttttattaat actttgctgc tgtggtggat
    39421 gcttagaata agaacacagt tattaaaaaa aggagggctt tgttatgtta gatagatttt
    39481 ttggagaaga aggatgcttt ggtggaggag gatggtggat aatagtacta ttcttcctat
    39541 tcttagcttt tgaagattgc tggagagaca ttgacataat gtcatggata cctttcttaa
    39601 tattattgct aataacttgt tcatgtggtg gattctttga tggtgaagta ggttgcggct
    39661 gctaatactt aaatcattag aataaaaaaa tgctggtatt ttataccagt atttttttat
    39721 tttatttttt aatatgattt aaattaagaa tgaatattat taaatttgtt gaatataggg
    39781 tataatataa aatataatgt atttaaatgt acttgctttt atggtaaaat aaaaacttat
    39841 ataaatgtag ttaaaatagg aaccttcaaa aatttggttc agttttagat aaatttagta
    39901 aaataaggag gatgaagatg aaaggcaata taacgataaa agatgttgct aaacaagcag
    39961 gagtgtcaat atctactgta tctagagtta taaatgattc aaaacctgta actgatgaag
    40021 tcaaacaaaa agttttagag gttataaaag agactggata tataccaaat ccacttgcta
    40081 gaagcttagt aacaaagaag agtcaattaa taggggtaat agttccagaa gtttcagatt
    40141 cttttgttaa tgaggtgtta aatgggatag aagaagttgc taaaatgtat gactatgata
    40201 ttcttttagc gaatacatac tctgataagg aacaagaact taagagtata aatctattga
    40261 gagcaaaaca agtggaaggt atagttatga tttcatggat agttgaacaa gaacatatca
    40321 actatataca aaattgtgga ataccagcga catatataag taaaactgct agaaattatg
    40381 atatatatac agtaagtact agcaacgaag aagctacttt tgatatgaca gagcatctta
    40441 taaagaaagg tcatgaaaag atagctttta taatgacgag taaagatgat actgttttag
    40501 aaatggaaag acttgctggt tatgagaaag cactttcaaa taacaatata gaattagaca
    40561 agagtttgat taagtatggt ggaactgatt atgagagtgg atacaatagt atgaaagaac
    40621 tattagatga tggaataata cctcatgcgg cttttgtaac aggtgatgag gctgccatag
    40681 gtgctataaa tgctatatgt gatgctggat ataaggttcc agaagacata tctgttgcag
    40741 gatttaatga tgttaagata gctagaatgt atagacctaa acttactaca gtatatcaac
    40801 ctctatacga tatgggagca gtagcaataa gaatggttat aaaattaata aataaggaat
    40861 taattgaaaa taagaaaata gaattacctt atagaattgt tgatagagaa agtgttacag
    40921 aaagaaaaaa ataaaactat taaaatcaat caggtagtgc ctaaggcact acctgatttt
    40981 ttatgtcaat aatatcaaat ttaataatag attaactaaa aataattatc taattactta
    41041 aatagtttat ttttatctac tcatgagggt tagacttttt agttttctta aataaacctt
    41101 tagatttttt ttgttgacca cctggtgtat tttttggtgc aggtgaattt gatttacctt
    41161 tagtaggttt agccttctta gttttagctg gtttagtatc tctttgattt tctgcatctg
    41221 agattttttc cattatatta tctagttttt tgcgttgatt ttcatctaaa aatgctttga
    41281 attttgatac tacttcatct cttccatcta agttcttacc tatttttata agttcctcca
    41341 taagttcatt ttctgatttt cctttgtaac tatcagccat cttttctatt ttatccttgt
    41401 caatattttt ttgtttagcc attttttcta atgactctgt atctaatttt ttcataaaaa
    41461 aacccctttc tataaatata tattgaatta tattcatact atttatatat agaaataata
    41521 ttgaagtggt gaattgtgag tgatatataa tataaataaa aattcttata gataatatat
    41581 atgcactgag ctttaaaaat ggaacattaa aatatgaaaa acatacttta atatagggca
    41641 attatataat ataaggggga attgtcttga ataacttact atttttgatt ggagtgattg
    41701 cattgagtaa tggtaatatc tctatatttg atgataaaaa taaatcaaaa ggaaataata
    41761 gagttagaaa aaacaaaaaa aatgcaaatg ataaactttc acagtctaca catggcaatt
    41821 taaggtttga tgcaaatgat ataaaaagag gtttaaggct catggattta agtgaagaag
    41881 atttagaaat gggaatggaa ataataacaa gaactaaaaa atacatgtca agagatgaaa
    41941 gaaaaatact aataaaaata gaaagtgttt tagatttagt tagaggaata aaaaaattaa
    42001 ataatataga tatggtagat acagaagatg aaacggattt tttcagaaaa atggatagtg
    42061 atgataaaaa gaatatgatg ataaaggaaa ttatagatgt attcccagaa aaaagaaaaa
    42121 attcagttga aaaagctata ggaatgaaaa agaagataga tttatttgca gaattatttt
    42181 taccagatga ctttggtgaa ggtggattta gtttgagctc tttggcaaat ataaacaatc
    42241 ttggtagtat gaataatctc aaattgttag gaagtctttt aagaggtgat gatagtaata
    42301 atgatgatga agacaataat gatgagttaa attatgaaga cgaagaatta gattgtgaag
    42361 acaaagaatt ggatgatgat gagttaacag atgaaggtga aatggaggat aatgaagatg
    42421 aattagtaga agataagaac acaggctatg aaaatataga aaatgatgaa gatatagtat
    42481 acgatgagga actatacaac attaatgagc aagaatccga atacaattat aatgaaaaaa
    42541 atactaaaac tagagctaag aaaaaacgat aattaactaa atacataaag aaaaaaggaa
    42601 gaaaaagtaa tgatagaata agattatctc aattactata taaaaccact ttaaaaaatt
    42661 caaaatttat tatataataa taatatgaaa tcctgaaaca tacgataagg cttttttaaa
    42721 ttcaacctac aaatgaaata cgggagaccg tgtctggtaa attataacat gcctagttag
    42781 tttggacgtt aaaggttact ggcaagtcac ccacctggga gtgcctaggg tgtgaattta
    42841 cttagccacc ggtgtttcgg gacaaaaaaa gtgctactat ttcaaatagt agtacttttt
    42901 ttattaatat gcatttttct actaaatacg cctaattatt gataaattca aaaagcaagt
    42961 aacaattaag attataaaag catagtatta attaggcttc aaaaagccaa agacttaata
    43021 agaatcgctt acagggaggg caatgaaaaa tgcaagatta taaaaaaaat aaaagaagaa
    43081 tgatgaatca gccaatgtct acaatgaatg aagaagaagt gtatacagat gaaataaatt
    43141 cagaagacat gagagggttt aaaaaatcac accatcataa tggatgtaat actgataata
    43201 agtgtgagtg ccatgatgag tgtaatccat gtaacccatg taatccatgt aaacctaacc
    43261 catgcaatcc atgcaaacct aatccatgtg atgacaaatg tgaatgccat gacaagtgta
    43321 aatgtgattg tgaaccatgt gaaatggatt cagatgaatg ttttgaaaac aaatgtggac
    43381 cagaatgttg taatccgata tctccaagaa acttctctgt atcaaatgca gtgccatttg
    43441 caatagaggc taatagaata tttgatacta tgcaattcca aacatttaca gatgcaacag
    43501 gtccaaatgg agaaccatta acttttgaaa cagaagtagt agaagtattt ggttcaattc
    43561 caagtgcagg tcaagcaagt gtaactatag aaaaaatatg cttaagtaat gatggaatag
    43621 ttatagacac aggaatgaca actttagaag atttcgattt agacccatta ggagatatag
    43681 taggaagaaa ctgtgaaaca acttttgaat ttgcagtttg tggagaaaga aacgctgagt
    43741 gctgtagaca aggaaaaggc aaatcagtag cttataaaca aagaggatta actgtagcag
    43801 ttcgtaattt agtactagag ctaagaggta gatgtggatg tacagagttc gttgcattag
    43861 ctttcccagc agttagagca ggaggtggat gtaagagaag agttgattat gtagaattta
    43921 cttttaacac actttcagca ccaatatgct tgccagctga cggaagagct gttactttaa
    43981 gacaagaata tcaaactaac ttaactgtag attgtatagg aaaatctata ttaaaattag
    44041 aatgcaacga atgttgtgaa cctttctatg aattaattat accaaatgat atagatttag
    44101 tactttgctt acaagaaaca gttagcacat taataagtga acaaatagta gttttagcat
    44161 caccaaatcc aatccaacca agacttgttg atactttctc taaagtatgt gatttttcgc
    44221 aatgtggacc taatcatgga agtggaaagc caagttgcca cagatagata ggtaagatat
    44281 atagacagtt taaattataa ataaaaatca tctaaggaag gctaccaaag tgtataattt
    44341 acattggtag ccttcacgtt tttataacta gcgtatagat tttagtgatt taaaaagcac
    44401 tatagtatgt aatagacaag taaagattga tactatcgtc attgtgtata tgtatgcata
    44461 aatattaagt cctggaattg gaagaaatac atataaagct attagttgaa ttaacatgcc
    44521 agtaatagta tttatacttg aagctaattc ttttcttatt gaatgtaaaa tgccagatag
    44581 agtttgattt agggacataa atagtggaac taaaaacata gcttttagat attctcctgc
    44641 aagtgtattt ttaaatacta atatacataa atcttttcca aagaaataaa aaaatattga
    44701 tgatagtatg cctacaaata aagtaagaag tatggagtat ttgatttttt taattacact
    44761 cttacgcttt cttagagcca tttcctgtga aatagttggt attaaattta caataagcgc
    44821 tgaaccaaca gtaaaaggta aaaatataaa gggcattacc atcccactga caataccata
    44881 catacttaaa gattgttgat aagtcattcc tgataatgct aatcttgatg gtatcatcat
    44941 agaacttata gattgcaaaa gtatgtttga cattctatta caagttatgg gaatagacat
    45001 ttttaatgtt tccatagaag aattataaaa atcttttatg cttataatat acctattgtc
    45061 aaatgatgaa tctcgccata agcaaataag cataaatata atattgctca actcaccaat
    45121 agatatacct aatagagcta tataacaatt catagattta ttatttatgt acataacaag
    45181 taaaaaaaca aataatattt taccaatttg ttcaatgatt tgtccaatag cagggatttt
    45241 tacattcttt atgccatagt aatatcctct tagtacgttt gaaagagtta ttataactat
    45301 agcaggacaa acagctagta taaatagatt taatttagca tcttttaaaa atttaagtga
    45361 taaaaaagaa ctattgaaag atgcaactat agatataatt agtgctacga aaaatgatat
    45421 atataaagtt gatataaata gagcattagt attgtgtcta tcgtttaaag cttttctctt
    45481 tgcaactaga caactaagtg cagtaggtat acctgttgtt gtaacagcta agcaaatcat
    45541 caaaaagtta aaaatcatat gatagatacc taaacctgtt tcacctagtg ctcttgataa
    45601 gaatatttta tataaaaaac caaatattct tacaataaaa ttggatataa ataaaataac
    45661 tgttgatagt actaaataag gtactttcaa aaaaatcacc tccttatctt ttaaatatat
    45721 tcacaaggag gtgtaaaaat ttataatttt ataaattctg gtttcaattt attaaattta
    45781 gatacatatt taaatcccat attttttagt aatgaatata tgtagtcaaa ctcacatgca
    45841 acttgactta tatgatgtga atctgaacct gtggttatga tttcccctcc caagtctttg
    45901 tacatttgaa gtatatactt agatggggtt aggtctggaa gattatatct ataacaagag
    45961 gtatttatct cgataccttt gccatcataa atagcttgtt taagagtttc ttcaattata
    46021 tcagaaaata gtctatcatc taaaatagtg tcataaggtg catacctttt aatcaaatct
    46081 aaatgaccta agacagagta atctttataa ttcttaacaa tattatataa ataaagatag
    46141 taattttcat aaacttcata ttgagtttta tctttaaaat aattaccaag gtataagtcc
    46201 attgtatcta tagcatggat agaacatatt ataaagtcca atggatattg atgtgcttcc
    46261 tttttgtatg tatccattaa ttgagtttga actccaaatt caattccttt ttttattgat
    46321 attttatctt tgtatttgct ttgaagagtc tctaaactct taaaataatc ttcgtagaca
    46381 attgaaaaag aatcatcagc atatacatca tagtcaacat ggtctgtgaa acaaatttcc
    46441 tccaaaccga gttcaataga tttttttacc atttcttcca ttgtagattt tccgtctgta
    46501 gagaaacttg agtgcatgtg atagtcgtaa aacataaata tttccccttt ttataaaaat
    46561 tttggtatta tatatacata ctaaataata tcaaatatat ataaaagcat caataaaata
    46621 taaatacaat gagcggtgat aaatttggaa aaatacagaa tagatttaaa tgagaaaaaa
    46681 tcaactaaat atatacagat atttaatcat ataaaagaat tgataataaa taaagagtta
    46741 gtagaacatg aaaaattgcc tccaataaga aaactatcaa attttttaca agtaaataac
    46801 actactatag ttaaggtata cgagctttta gaaaatgaag gatatgtata taaaattata
    46861 gggagtggta cttttgtatc aagttcaaat tcttataaaa gcggtataga taacaggaaa
    46921 aataagatta ttgtgcaaag agaagattta attcattttg acaatgggaa tccatctaat
    46981 gatatgtttc ctatcgattc atttaaaaat gctgtaaata tggcattatc taaagatggt
    47041 agttcaatat ttgaatatga tgaaggtctt ggctctgaag aattaagaga aaaaatggta
    47101 gagtatctaa gtgatggaaa tataaaaact actaaggaaa atattcaaat aatatcaggt
    47161 gctcaacaag gtatagatat agtttgtaaa ggacttataa gttattcaga tgttgttttt
    47221 atggaagaac cttcttacaa tggagcaatt gaagtgttta aaagtagagg tgcaaaaata
    47281 atatctatac ctatgttaga tgatggaatt gatataggta ttttaaaatt aaagttggaa
    47341 aaaataaaac caagacttat atacattatg cctaattttc aaaatccaac tggaatatct
    47401 tactcaacat ataaaaaaaa gaaattgata gaattggcag aagaacatga tttttatata
    47461 atagaagatg attttataag tgattttgtt tttgaatcaa aggacaatag aacaattaga
    47521 agttatgatg ataaaaatag agtagtatat ataaaaagct tttcaaaaat acttatgcca
    47581 ggtcttagga taggaattgt agaaatgcca aatgagttgt taaaaaaagt tttatgggct
    47641 aaatattcat cagatatatc aacaccaggt ttaattcaaa aatcaatgta ttactatatg
    47701 aataattttg attggaagaa ttatttgagt catatagaga agatatatac taataagtat
    47761 aaattgtcaa aaaacttgat taatgacaaa ttgagtggta aattaaaagt tagaaaatct
    47821 tgtggaggaa taaacttttt tttagaatta ccaagaggat atacatctca agcatttaca
    47881 aattttatgt taaacaaagg agtgtcaatg cttccaggaa cttatttttt tgacaatcta
    47941 gtggatgata gattctttag gataaatata gctcgcccaa gcatggaaga actagaaaag
    48001 ggaatatctg taataagtga taatatagat gagttttttt gtgaatataa aaacaagtta
    48061 gatataaaaa gcaataaatt attttattaa ataaatagag gttttttagg aggaaaaaat
    48121 gtttgacaag aagaaattag ataggattaa tgaattagca aagaaaaata aagagggaat
    48181 cctttctgct gatgaaatca aagaaagaga aattttaagg aaagaatatt tagaaaactt
    48241 tagagctcac tttagatcaa gactagatag tgtcaaagta gtttcaccag aagaatatga
    48301 gcaatacatg aaaaacaata aaaattagaa cttagtagga ggaaaatatg aacttaaagt
    48361 acgaatttaa gaatgcttgg gattttgaaa gaaaagaaaa tactattgaa caaattatgc
    48421 aatattctcg caattatatg gagtttttaa gtaagtcaaa gactgaaaga ctttcagtaa
    48481 aagaaattat aaaattggct aaagaaaata actatatttc aatagatgaa gctatggaaa
    48541 aaggaagcat aagttgtggt gataagattt atgttataaa taaagagaag gcagtagcac
    48601 tttttgtaat aggtaaaaat tatatagaaa aaggtatgaa gattatagga agccatatag
    48661 attcaccaag attagattta aaaccaaatc cactgtatca agagagtaat ctaggttttt
    48721 ttaagactca ttattatggt ggaataaaaa aataccagtg gacagctctt ccacttgctt
    48781 tacatggtat agttatatta aatgatggaa caaaagtgga catatctatt ggggaagaag
    48841 atagtgaccc agtattttgt gtaactgatt tattgattca tttggctgga gaccaaatgc
    48901 aaaagaaact atcagaagga attagtggag aagctttaaa tatattaatt gggaatattc
    48961 ctttagaaga tgaagaaaaa gaacctataa cagcaaatat attaaagatt ttaaatgaga
    49021 aatacaatat agttgaagaa gacttattaa gtgctgaaat tgaagtagtt ccagctggta
    49081 aggctagaga tttagggttt gataggtcta tggtgttggg atatgggcat gatgataggg
    49141 tatgttcata tgctgctgta aaagctatac tagaaactga acaaccagaa tttacttcag
    49201 ttgcattatg tgtagataaa gaagaaattg gttctaaagg gaatacaggt atgcactcaa
    49261 agttttttga aaatactgtg gctgaactga ttgctttaga aggagattat tgtgatatta
    49321 aagtaagaag agctttagca aatagtaagg tattatcagc tgatgtttct gctggatatg
    49381 acccaaattt tggagaagca tatgaaaaga gaaattcagc atatatggga aatggagtag
    49441 ttttaactaa gtatacaggt tcaagaggaa agagtggatg taatgatgct aatgctgaat
    49501 ttatgtcaga agtaaagaga atttttaata aaggaaatgt agtatggcaa actgctgaac
    49561 ttggaaaagt agaccaagga ggaggcggaa ctatagccca tatattggca aatcaaggtg
    49621 cagaggttat agactgtggt gttggagttt taaatatgca tgcaccacat gagattgtct
    49681 ctaaagtaga catatatgaa atgtataagg gatataaggc attttttaat ataaatttat
    49741 aagtaaaaat ataaatgtaa taagtgaata aaggctgttg tataaacaat ttaatctgtt
    49801 gtacaacagt cttattttta tgtaaaaata gcaatttgtc acactagaga aaataaaaga
    49861 aaatactgtc aaattaaaaa ttatttaaaa aaaaattgac aacgatttca tcttgttata
    49921 atatgtaata aagaacttgt tatataatgt tataaaaatg ttacataggg aggtgagcaa
    49981 aatggatgaa aagagacaaa aaaaagaaat ggatttagaa gtaaattcct ttgttccttt
    50041 atatcaacaa ctatatgaca acataaaaaa acaaatagca tctggcatat ataaaccagg
    50101 agataaactt ccatctgaag gagacttatg taaagaattt aatataagtc gtataactgt
    50161 gagaaatgca cttaatgaac ttgtaaaaga agatatatta tgtaaaaaac gtggtaaagg
    50221 gacttatgta acaataccag agagaataga agcaacatgt gcaggaaata gttttactaa
    50281 ttcttgtcat cgtattaatg caaaaccaag taccaaaata atctctgttt taataaaaaa
    50341 agcagataag caagtagctg aagctttaag tattgaacta gaagagaagg taatatgtat
    50401 taaaagactt agattaatag atgaagtacc tgttattttt gaagttgact attttagaat
    50461 agattacatg tttttattaa aagaagaatt agaagataaa tctttaatgg aggttatatc
    50521 taataatata agcacattac ctaaacgagt agaaaatata tttgaagtaa aacattcaaa
    50581 caaagaatat tctgactatc ttaaatgtgc aagtaatatg ccactattga aagtaagaca
    50641 atcggtgtat acagaaaata atgatgtctt atattacaat gagcaattta taagaagtga
    50701 taagtacaaa tatgcagtat cagcagaaat ataatctata tttagtagga ggatgaagta
    50761 tgaaaaagat aatattagca tctcatggag atttttcaaa agggctttta aatgcagtaa
    50821 aaatgatagt aggagattta gcagattgtg ttagttcgta tggtttgtac cctggacaaa
    50881 gtgcttcaga ttttgcttta gaattagaaa aaacaatatc agaagatata gaatgtgaat
    50941 atataatttt atctgactta tatggagcaa gtgtatgtac agcaatgtta agattaacta
    51001 atctacataa tgttaagtta ttttctggaa tgaatttgaa tatggtatta gaacttctta
    51061 caaggttttc tgatgaatta acagttgaag atatagaaca attagtggaa gaatctagaa
    51121 gaggaataca aagcgtgaca ttacaaatta aagaagaaga agaggtattt tagtttatga
    51181 aaaaaaaggg aatattatca gtatttaaaa gtaaaaaacc aattatagca atgatacact
    51241 taaaaggaga tactccagaa gatatctttg aaagagctaa aaaagagatt acaatctttg
    51301 aagaaaatgg tgtagatgga ataatgttag aaaattacta tggtaattat tatgatttag
    51361 aaagaatact tgaatatgta tcaaaagcta atttgtcaat accatatgga gtaaattgtt
    51421 taaatgttga tactatggga tttgaactgg caacaaaata taatgctagt tatatacagg
    51481 ttgattctgt agtaggtcat gttaaaccta gagacgaagc tactttagaa gaatttttta
    51541 aattacaacg ttcaaaatgc ccagcgtatt taattggagg cgtacgtttt aaatatcagc
    51601 cagtattatc agaaaatgac gttgaagaag atttaaaaat aggaatgaca agatgtgatg
    51661 ctatagcagt tacagaaaat gctacaggtc aagaaacatc aatggaaaaa atagaactgt
    51721 ttcgtaaaaa tttaggagac tttccattgg ttatagctgc tggagttaca ttagaaaatg
    51781 caaaaaaaca gcttgaatta ggagatatgg caataatagg aagttacttt aaagataatt
    51841 acaaagattt tgggaatgtt agtgtagaac acgtaaaaac attcatggat gaaatcaaaa
    51901 aaattaggga ggaattataa tgattaaatt agtaagagta gaccacagac ttatacatgg
    51961 acaagtagca tttacatgga caaagttttt aagcacagac tgtatattga tagcaagtga
    52021 tgaattatta aaagatgaat taagaatggc agggcttaaa atggctaaac catctaatgt
    52081 taagttagta atgaaaagca tagcagattc tataaaagca cttaactcag gtgttactga
    52141 taaatataac ttattgatac tttgtgaatc tgtagaagat gtttatagac ttgctaaaga
    52201 ggtaaaagct attaaatcta taaaccttgg tggaacaaaa tcagatgata atcgtgaaaa
    52261 tatatctaaa gcagtgcatg tatcaaaaga tgatataaag atgattaaag agttagattc
    52321 agaaggcgta aatgtatttg tacaattagt tcctgatgat gatgctacaa atgtaatgaa
    52381 attaatataa ttttaggggg atgaaaatgg aatttacaca agttattttg ataactctaa
    52441 ttgcattctt tgcatatatg catagttttg taggttctac aatgcataat agacctatag
    52501 ttgttgcacc acttgttggt ttagcactag gtaatcttca tacaggtata gtaataggtt
    52561 caacattaga attagtattt atgggtgcat ttcctgttgg ggcaagtaat cctcctgatt
    52621 ttgtgtctgg ttctattatt gccacagcgt ttgtaatatt aacgggtcaa gatgtatcag
    52681 cagcagttgt acttgcagtt cctatagcaa ctctagtatt gcttatagat aacttcttaa
    52741 tgacagtagt acttacatgg ggagctcata ttgctgaccg ttatgcagaa gaggggaata
    52801 ttgaaggcgt agaacgtgtg caattattat ttggtatagg aaataaatta atattagcaa
    52861 taatagtagg tataggattt tcattaggtg ttccagtaat agaaaaaata ctttcattta
    52921 taccatcata tgtaactcat ggaatggatg ttgcagcagg cgtgattcca gcaataggtt
    52981 ttgccatgtt agctaggatg atgcttaata agaaaacagt agcattttta ttattaggat
    53041 ttatattggt tgcttaccta aacataacag taacaggagt tgcattgttt ggattagcaa
    53101 tagctttaat atatgttaac tttgtaggag agaaggaggt aatagtagat gacaacgagt
    53161 tctaagaagt tagatacaat ttcacccgat agtaaaataa cgaggaaaga cttttggaaa
    53221 tgctttagaa ggtcattgac attagattca tcttggaact acgaacgtat gcaaaatata
    53281 gcatatgcat acatgatggc accaataatc cgtagattgt ataaagatga taaggaaaag
    53341 aaatctaagg ctttaaaaag acatttagaa tttatgtcag ttactcctca tataagtact
    53401 cttttggtag gtatatctgg agctatggaa gaagaaaatg caaagaataa agaatttgat
    53461 gctaatagta taaatgctgt taaatcaagt ttaatgggtc cagtttctgg gataggagat
    53521 tcatttttct ggggaacatt aaagcttata gcagctggag ttggtatagc tttagcttct
    53581 cagggtaaca taatgggacc gattttattc cttttaataa taaatgttcc tcattttatt
    53641 atacgctata tatgtctaga taaaggattt aagtatggaa ctcaattctt taaagatgtg
    53701 agtagttcaa gtatagtttc aaaagttatg gaagcagctt caatgttagg gcttatggta
    53761 attggaggta tgactgcatc taatgtaatg ttaaaactta gtgttaatgt aggtagtgga
    53821 gaatgggcag agcctattca aacttactta gaccaaataa tgccatgtat gcttccagct
    53881 atgatattcg gtattatgta ttggttatta ggaaaaaagg taaagacaac tacgatatta
    53941 atttcagtta tgattatttg tatagtatta gctgctattg gaatagtata aataaaaaaa
    54001 tcccaagttc atttgaacga gggatttttt gactaaatta aataaaaaag aaagatggtt
    54061 cataataatt tataagcatt tatgtgatag ttataattag cttattaatt agttataaac
    54121 ccaaaattaa gcactacttt attaaagtta ttatattata atttcgaaat actcacataa
    54181 tctgtttaga aatttatctt ctaatgggga aaaagttttc tttttagagt aaataaagaa
    54241 aaattttcta gtaaaatcaa caccctttat tttataacat tttattttac ctgcattgag
    54301 ataatcaata gctgaaatgt acgatataaa ggagactcca agacctaatc taaccatttc
    54361 cttaatagcc tcatttgatt ctacatgagc tattacattt aatttactta ctggaaagtt
    54421 atttttacta agtgtgttta gtattaagtt tcttgtacct gacccttctt ttctcataat
    54481 aaaatttaga taagccaatt ctcctatatc gatacaatta tttttattat caattttaaa
    54541 atctgaagga gtgataagaa caagttcatc atctaataag tctaggtact ctatttgagg
    54601 attattgatt ttagaaccaa ctaatccaaa gcttattcgt tcatttaaaa tttctgatat
    54661 agcatattga gaatcataat ggcttatgga aaattttaca tctggataac tcatagaaaa
    54721 actcttcata aaatcaggta aaatataagt ttcaggtatg gaactacatg ctatatctat
    54781 aataccttct atttttcctg aatattcttt tatatcataa attgcacgtt tgcaattatt
    54841 caatatatat atagcatgtt cataaagaat ttcaccagat tttgtgagtg taataacttt
    54901 gttgcttcta tttataagaa cagtttccaa ctctctctct aaattctgta tgtgtgcact
    54961 tacagtaggt tgagttaaaa aaagttctct tgcggccttt gaaaaactct gatgttttgc
    55021 aacagcaaca aatacttcaa gttgtttaaa atccattatt taatcctcct taaattatct
    55081 aaaactatta taacatattt gataaattaa tagtcaaagg tgatttttaa aatagaaaac
    55141 ctttaaatat tcatggagtt tttgattgtt taggaataat ttggtataat attgtaaggt
    55201 acttacaata aaaatatcgt aaattgaatt ttcaaaattc aactttaaag tgttttttat
    55261 tacagaaaaa ttacaattag cttcttaatc ttatt
//
LOCUS       DAMJWP010000008        50327 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.008, whole genome shotgun sequence.
ACCESSION   DAMJWP010000008 DAMJWP010000000
VERSION     DAMJWP010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 50327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50327
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.008"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(78..683)
                     /locus_tag="PC700_000422"
     CDS             complement(78..683)
                     /locus_tag="PC700_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF2579748.1"
                     /translation="MQNTAKRGNMISTRTLVVMSILSAISYLLMFIQAPIPGIFPDFL
                     KIDLSDIPAIFGGMSLGPLVGFGIVVVKNVLQAITATTTGGIGEFANILIGGSYVMIL
                     CYSYKRSGDLKGVLIGFVLGTISMTIIGSLVNYFVMLPLYGQLMGLDAIIGLGSAINP
                     QVHDLFTFVIWMIAPFNVLKAVIISLVTLPLYKKMGNIIKK"
     regulatory      complement(808..925)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(985..2151)
                     /locus_tag="PC700_000423"
     CDS             complement(985..2151)
                     /locus_tag="PC700_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2579749.1"
                     /translation="MIFIKNGKINTITNGIIHGDILIDEGKIIEIGEDLIAPLDVEVI
                     DASNKLVFPGFIDAHTHLGLWEDGIGFEGADGNEETDPITPQLNPIDGINPMDRTFKE
                     AFEGGITSVCTTPGSANVMGGQCIAIKTCGKRIDKMVIKNPVASKIAFGENPKSCYGQ
                     DDKSPQTRMAIAALLRENLKKAEEYLEDIDMYESHDDEDCEKPEYDIKMESLIPVLRR
                     EIPFKAHAHRADDMFTAIRIAKEFNLKLTLDHCTEGHLIVDELVEEGFPVIVGPSLSE
                     RSKFELRNLTFNTAGILSNAGLDVCIMTDHPVIPVQYLPICAGIAVKHGMKEEKAIES
                     ITINPAKTLGIEDRVGSIEVGKDADLVIWDNSPLEIQSNVLYTIINGKVVYEKK"
     gene            complement(2299..4440)
                     /locus_tag="PC700_000424"
     CDS             complement(2299..4440)
                     /locus_tag="PC700_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDF2579750.1"
                     /translation="MDINQILKKEFNLRDEQINNTLKLIDEGNTIPFIARYRKEMTGE
                     MSDVTLREFYEKLMYLRNLQSRKDDVVRLIDEQGKLTDEITQNIEKAKTLQEVEDIYA
                     PYKQKKRTRATIAKEKGLENLALSILENNLDNIEIEAKNYLDEEKEVLSIEDALKGAR
                     DIIAELVSDDAKIRKYIRELALREGMIVSKSATDEKSVYDMYYDYNEAVKSMAPHRVL
                     AINRGEKESFLKVKLEINNDKVLNYIINEYVNDKNFKNKEEIVSSIEDSYKRLIFPSI
                     EREIRNHLTEIAQERAISVFGKNVKSLLLQPPVKDKVVMGFDPAFRTGCKIAVVDKNG
                     KLLDYTTVYPTDPQNDVEGAKKVLKGLIEKYDIDIISIGNGTASRESETFVSEMIKEI
                     DSEVQYVIVSEAGASVYSASELANEEHPDINVSIRGAISIARRLQDPLAELVKIDPKS
                     IGVGQYQHDLNKKRLEEVLDGVVEDSVNSVGVDLNTASYSLLEHVAGISKAIAKNIIA
                     YREENGDFTSRAQLKKVKRLGPQAFTQCAGFMRILEGKNPLDNTGVHPESYDICKKMI
                     EIIGYSLDDVKNKNIGEIDEKIKEIGLRELSEKLEVGQVTLKDIIAEIKKPGRDPREE
                     GIKPILRTDVLKIEDIQEGMTLKGTIRNVVDFGAFVDIGIKNDGLVHKSEMSNSFVKD
                     PMSIVTVGDIVDVKVIGIDLNKKRVALSMKK"
     gene            complement(4584..5691)
                     /gene="prfB"
                     /locus_tag="PC700_000425"
     CDS             complement(join(4584..5615,5617..5691))
                     /gene="prfB"
                     /locus_tag="PC700_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153309863.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDF2579751.1"
                     /translation="MLKLQEYRHSMEQMASNLEELRVSLDIASLMKNIQINEEKMNQQ
                     DFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIGLEEDDDSVERDIEKS
                     IESMEEKLSEMKIKTLLNGEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKG
                     YKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHRLVRISPFDPSGKRHTSFA
                     SIDVTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQN
                     ERSQHLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQIRSYVFQPYKLV
                     KDHRTNAEFGNVDSVMNGNIDLFINEYLKMNKIV"
     gene            complement(5706..8381)
                     /gene="secA"
                     /locus_tag="PC700_000426"
     CDS             complement(5706..8381)
                     /gene="secA"
                     /locus_tag="PC700_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDF2579752.1"
                     /translation="MSFMDNLFNMADKKELKKFNKTVDIIDSLEPKFESMADSELKNM
                     TNIFKERLANGESIDDILPEAFAVVREVSKRVLGLRHYRVQMIGGIVLHQGRIAEMKT
                     GEGKTLVATAPVYLNALTGKGVHVVTVNDYLAKRDRDQMAKIYEFLGMSVGVIIHGQN
                     PKVRKEQYDCDITYGTNNEYGFDYLKDNMVIHKEQRVQRGLNYAIVDEVDSILIDEAR
                     TPLIISGPGDKSTHLYSDANTFVLTLKPDDYELEEKDKAVSLTASGIQKAEVYFNVDN
                     ITDISHTELYHHINQALRAHVIMKKDVDYVAKDGEIVIVDEFTGRLMFGRRYSEGLHQ
                     AIEAKEGLKIQRESKTLATVTFQNYFRMYKKLSGMTGTAKTEEEEFKAIYKMDVFQVP
                     TNKLMVREDLPDSVYKSEIGKFNAVAQEIIERHKVNQPILVGTVSIEKSELLSQILKK
                     KGIKHEVLNAKHHDKEAEIIAQAGRLGAVTIATNMAGRGTDIVLGGNPDFLTKREMRR
                     NGFKEEIVNRVDTPIEGIPVKGNEILFEAREEYEKLFEKFKQQTQEEQKQVVEAGGLA
                     IIGTERHESRRIDNQLRGRAGRQGDPGSSRFYIGLDDDLMRLFGSDRISGIVDKIGLE
                     EDMPIEHRILSKSIEGAQKKVEGKNFGIRKHVLQYDDVMNKQREIIYAERKRVLEGED
                     LQEQIQSMTHSIIEEAVTLYTQDKGFDEEGFKEHMYNLFLPKGSIEIPEIEKLNPVEI
                     TEKVYEIAMKIYTSKEEQVGYERMREVERVILLQAVDNHWIDHIDAMDQLRQGIGLRA
                     VGQQDPVIAYKMEGFDMFDEMNKHIKEDTVRYLFNITIETPVERKAVVDVENLSSPSD
                     GTLPTSKTVKKDEKVGRNDLCPCGSGKKYKNCCGR"
     gene            complement(8513..9064)
                     /gene="raiA"
                     /locus_tag="PC700_000427"
     CDS             complement(8513..9064)
                     /gene="raiA"
                     /locus_tag="PC700_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDF2579753.1"
                     /translation="MNIIISGKQIELTDAIKNKIETKLSKLDNYIHPDTDVKVTVSAR
                     KARQKIEVTIATVNGPIIRAEDMQENLYTAIDVVYDKLSKQLKKYKKRLQDKHKDNKS
                     IRFQDSEINLADEQEDYGDENQELVIQRRKKFSVKPMSEEEAVLQMELIGHDFYMFKN
                     IDSDEIAVVYKRNNGGYGIIEHE"
     gene            complement(9617..10291)
                     /locus_tag="PC700_000428"
     CDS             complement(9617..10291)
                     /locus_tag="PC700_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HDF2579754.1"
                     /translation="MLEIIGMSVEDAKIIEDCGADRIELVSALTEGGLTPSFGLIESV
                     VNSVKIPVNVMIRHHAKSFVYSKEDISIMQKDIGVVKEIGANGVVFGVLDKNNNIDEK
                     NLNVLLKCCDNLDVTFHRAIDESNTIDSVKILKDYDKITNILTSGGKGSIVDNIQMIK
                     NMMLSSNHIKILLGGGLNFNNIEKIKELTKASNFHFGTAIRINNSPFEDIDRQKLKQL
                     VNIISR"
     gene            complement(10294..11346)
                     /locus_tag="PC700_000429"
     CDS             complement(10294..11346)
                     /locus_tag="PC700_000429"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HDF2579755.1"
                     /translation="MNIISNKIDELKEDLLSDIIDIVKIPSVKGESENGFPFGEKVGE
                     ALNKALEISKKLGFKVRNLDNYIGYAEHGDSDDYVCVIGHVDVVHEGDGWKHQPYKGE
                     ETNGRIYGRGVLDNKGPIMSALYGLYAIKELNLKLDKRVRIIFGTNEESGFEDIPYYL
                     EKEKTPIMGFTPDCKYPVVYGEKGMAKIRIKSKINYEEDVYLGFIENMSENVLVTYKE
                     LNIENSNTILDIKVKYDFSYKLKDVLDEIKASFPNSIDIEVISNFNPVYFDKESNLVK
                     KLQLAYERVTSLDGTPVTTNGGTYAKVMPNIVPFGPSFPGQKGIAHNPDEYMDIEDII
                     LNAKIFANAIYELAKE"
     gene            complement(11394..12353)
                     /locus_tag="PC700_000430"
     CDS             complement(11394..12353)
                     /locus_tag="PC700_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HDF2579756.1"
                     /translation="MWGMIATWRMALEGITEASEHLEKGMEAGDAVELAVRRVEDYPF
                     YKSVGYGGLPNENCEVELDAAFMDGNTLDIGAVAGIRDYSNPVSIARKLSYEKVNNFL
                     VGIGAEDYAHKNGFERKNMLTDRAKLHYKKRKKETLDKGLSPYAGHDTVGMISLDKTG
                     KMCAATSTSGLFMKKRGRIGDSALSGSGFYVDSSIGGATATGLGEDLMKGCISYEIVR
                     LMKEGKHPQEACDIAVNELDKQLIKRRGKAGDLSLVAMNNKGEWGVATNIVDFTFSVV
                     TKEESPAVYISNRVDGKTIHKVISNEWMEAYEARIKAPIEVSE"
     gene            complement(12449..12967)
                     /locus_tag="PC700_000431"
     CDS             complement(12449..12967)
                     /locus_tag="PC700_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HDF2579757.1"
                     /translation="MKKIVMILDQIQAGAGGKEKSNIPPAGKSSPLGPGVMMEQFLNE
                     SKVIATLFCGDEFFVNNEKEVTSKMIAMVKKLNPDVVICGPSFNYENFSKMSAILSKN
                     INDKTDIPAFAAMSEENIDVINEYKNDICIVKTPKKGGIGLNDSLNNICKLAKAIANK
                     EDITLMKEEFCY"
     gene            complement(12978..14312)
                     /gene="celB"
                     /locus_tag="PC700_000432"
     CDS             complement(12978..14312)
                     /gene="celB"
                     /locus_tag="PC700_000432"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDF2579758.1"
                     /translation="MNRLERILMPLAEKIGKNKLLIAIRDGFLISSPLLIIGSLFLLV
                     ANFPIKGWNEFVGQIFGPDWAVKLQQPTVATFEVMTLLAVLGIGYSYAKQIDVDPIAS
                     AAVAIVAFFIVTPFVIPYTPEGTESVYMVTGIPLGWMGSKGMFVGMITAITSVKLFAA
                     VVKKGWTLKMPDGVPPTVSKSFAALIPSAIVMVVFFLVKIAFEATPYGSIHEFIFKFL
                     QMPLLKLGNSLGAMAIAYIFLHLFWFFGINGSSVVGAVYNPILKILSAENLAAFQAGS
                     KIPNIITGQFQDMFATFGGAGSTLSLVIAMLLICKSKRIKSMGKLSILPGIFGINEPL
                     IFGLPIMLNPILLIPFAIVPTINIIIAYFCMSAGLVPLTNGVQLPWTTPIIFSGFLTT
                     GWQGAVLQLVLLILGIFMYIPFIKMLDKQYLREENETVEEEDDDISFDDLVL"
     gene            complement(14351..14677)
                     /locus_tag="PC700_000433"
     CDS             complement(14351..14677)
                     /locus_tag="PC700_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2579759.1"
                     /translation="MKRKVYLFCSFGMSTSLLADKMQKVADEHNLPIEVEAFPIAEID
                     KIVEEKNPDCILLGPQVKYMLKELKPKFEAQGKLIDIINEVDYGTMNGEKVLKLAIKL
                     IKSKQA"
     gene            complement(14702..15010)
                     /locus_tag="PC700_000434"
     CDS             complement(14702..15010)
                     /locus_tag="PC700_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2579760.1"
                     /translation="MEEIVLKIIIHAGNAKSMLYEALDYAKENDFKKADELIENANEE
                     ILKAHKVQTELIQKEAGGDKSDISILLIHSQDHLMTCMSERNLIKEMIMLRKEIQKLK
                     "
     gene            complement(15151..17064)
                     /locus_tag="PC700_000435"
     CDS             complement(15151..17064)
                     /locus_tag="PC700_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2579761.1"
                     /translation="MICLNKRQENLLTTLLLKEEFINVNKIANDFECSERTIRNDCKY
                     IDEWLSTFCNAYIKRKPNIGIKFSGDGTDKILVNQKLRGEVKRVNSELHKQVEILNLL
                     LCNNKKVTLNDLSNKLYINKNIVREEISKLSVALNNYNLNVSTKKGSGIYIEGDEKDI
                     RIMLVSFLFKYIDKYRLNIDEIEYFHIVDIIIVKNIMLTIEEDMDIRLTDISFKQLML
                     FLLINIIRIRLGNSLKVSNTKKELKLKMLILDIEDELKSNLSIVLNYEEKLFIESLIL
                     GMNKQINSNNIKKSLYLNEELASYTKKIINLVSEESGINFNNDILLYEQLICHLNVTM
                     HQMKSNVYLENPLLDNIKTKFCFLFTVISSSIEIELKGKQITEDEIGYLTLHFQTSLE
                     RKYSKRESNKKASIVCPFSFGVSMLLKVKIEKRFDNIKIIETLREEDLKRDNFNKTID
                     FIIAFQEYENIQKPIFITTPLFTDEDENKLKEFTNKIKEKDTSYKLMNRLMMSDYLIR
                     ELEHDDIYEAITYLTNLLIEKHYAEKEYLQSIITREKSYPTNVGKGILFPHGDMKYIK
                     QSVLCFARLKTPIKIRNGKDIKIILLLAYKKDDDRKVFRELFKEISNLTEDENMLDIL
                     SKCDIEKVKDILI"
     gene            complement(17396..18178)
                     /locus_tag="PC700_000436"
     CDS             complement(17396..18178)
                     /locus_tag="PC700_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HDF2579762.1"
                     /translation="MKICNDNFIKNFKNMINKCLDFIYPENISCIICDKSIKKTNTYS
                     ICKSCFKEMNFIQDGCMKCGKPIIRHSIEKEFIEECSYCFNKDFYFDKSISCIEYNDV
                     TKKMVLGLKYNQKTFMAKYIAQIMKEKLYLENIKFDYILFVPLHKKRLNKRGFNQAQK
                     IAFNLSKIVNIPLLDCISRKKYTRMLYKLNKKERKEELKNVFVVKENVKLINNKNILL
                     IDDIFTTGLTTNEISKLLKLSGANKVFVLTLLTKANDNYIME"
     gene            complement(18181..20400)
                     /locus_tag="PC700_000437"
     CDS             complement(18181..20400)
                     /locus_tag="PC700_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HDF2579763.1"
                     /translation="MEKLEGMISEIVFKNEDNGYTIAHLVNENDEIVVVGCMPTLAIG
                     ESIEVEGKWVNHKIYGTQFEVNSFMPVTPSSLEGIYVYLSSGMIHGIGEKMAKRIIDK
                     FGVDTLEVIQNSPEKLQEVEGIGSKKVKQIVKSYEEDRELRNIIIQLSPFGITPNYCL
                     KIYKKYKSSAIEVINKNPYQLAEDIRGIGFKVADSIASKIGIDKNSKDRICQGILYTL
                     NKSLSNGHTYLPEHVLIQDSEKLLELNGEIIKECVMMLVYNQKIHIEKVNNENLIYLM
                     PYYLAENGVCSQIVKLSQYEFEDLKIDIDNEINVLEEDKKIKLAEKQILAVKESVNSG
                     VLIITGGPGTGKTTTINAIIDIFENNGKSVTLAAPTGRAAKRMSETSNKEAKTIHRLL
                     EMGFSTDDDLTFFKDEEDPINSDVIIVDEVSMVDIILMYNLLRAIKLGTRVILVGDSD
                     QLPSVGAGNVLKDMINSNIINVVKLNEIFRQAQESMIIVNAHKINNGEPLYLNTKGKD
                     FFFIRKSTNEEILNEIIGLVNERLPKFYKVDKLKDIQVLSSMRKGELGVTNLNIELQK
                     YLNKKEKFKVEESFSKRLFRVGDKVMQVKNNYTKKWETEDQKESGEGIYNGDIGYVYH
                     IDKDKKTIYILFDQTKIVSYLYDELDEIDHSFCTTIHKSQGSEFPVVVLPIAWAPPML
                     LSRNLLYTAVTRAKKLVVLVGDVKYLEYMIKNNRVNQRYSNLGYKLNKFKQEGLLIE"
     gene            complement(20664..21761)
                     /locus_tag="PC700_000438"
     CDS             complement(20664..21761)
                     /locus_tag="PC700_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2579764.1"
                     /translation="MSGKVNIKTVISFAGAYVATVIGSGFATGQEILQFFTFYGYAGI
                     IGGIISMVLFSWFGAEVIDKGRELKLKEPIKIYQVYCGKYLGTFFEWFGPLFLFGTLV
                     IMIAGAGATLSEYYGLNPYVGRIGMAIVSLITVSLGLTRLSKILGNIGPIIIIFTLLV
                     GAISLFSNIDALSNAGNMVDSLNIKTATSNGYFSGVLYTCYNVIIVITFLTGMGASAV
                     NKKDAVWGGIVGGVALMAAAIMMNLALLSNIGNIYTKEIPALYLADKISPIIGILFSV
                     VLLLGIYTTAVPLLWSVTNRFVEDDHPKFKIITIVVSILACIGGLLPFDKLVGTLYPY
                     TGYMGILILLCMLYRRITKTEGYKENKSEIS"
     gene            complement(22077..23270)
                     /locus_tag="PC700_000439"
     CDS             complement(22077..23270)
                     /locus_tag="PC700_000439"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDF2579765.1"
                     /translation="MARHLFTSESVTEGHPDKICDQISDSILDALLEKDPQSRVACET
                     TVTTGLVLVAGEISTSAYVDIPKLVRETVREIGYTRAKYGFDCDTCAVITSIDEQSGD
                     IAMGVDEGLESKTGEEIEEEIEKVGAGDQGIMFGFACNETPELMPLPISLAHKLSRRL
                     TEVRKTGLVDYLRPDGKTQVTVEYEGSKAVRVHTVLISAQHCETVSNDKIREDLINHV
                     IKEVIPAELLDEETKIYINPTGRFVIGGPQGDTGLTGRKIIIDTYGGYSRHGGGAFSG
                     KDPTKVDRSAAYAARYVAKNIVAAGLADKCEIELAYAIGIARPLSIFIDTFGTGKVSE
                     EKLVELVNKHFDLRPGAIIRDLDLRKPLYKKVAAYGHFGRTDIDLPWERTDKVEQLRK
                     DALGE"
     regulatory      complement(23407..23510)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            23699..24208
                     /gene="yyaC"
                     /locus_tag="PC700_000440"
     CDS             23699..24208
                     /gene="yyaC"
                     /locus_tag="PC700_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HDF2579766.1"
                     /translation="MYLAKVNYSDDDVIQMLSSVLKTIINENTIVVCIGTDRAIGDTL
                     GPLVGTILKNSNFKYPVYGTLDNPIHALNIYESLDTIKNTHIQGNFLAIDACLGSQSN
                     IGNIQIREGPILPGKGVGKKLPQIGNYSIVGIVDKIDENNKLSFNNIRLSFILDLAET
                     IALALLVST"
     gene            complement(24319..24747)
                     /gene="fabZ"
                     /locus_tag="PC700_000441"
     CDS             complement(24319..24747)
                     /gene="fabZ"
                     /locus_tag="PC700_000441"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDF2579767.1"
                     /translation="MLNIDEIKKLIPHRYPFLLVDKITELEVGKRAVGIKNVTVNEPF
                     FQGHFPEYPLMPGVLIVEALAQVCGVAMMSVEENKGKLGVFAGIDKVRIKREVRPGDT
                     LTMEIEMTTLRKNIAKADAKAYVGEELVCKGELMFALVEK"
     gene            complement(24763..25782)
                     /locus_tag="PC700_000442"
     CDS             complement(24763..25782)
                     /locus_tag="PC700_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HDF2579768.1"
                     /translation="MAGADIGIDLGTANVLVYVSGKGIVLEEPSVVAIDKRTDSVLAV
                     GEEAKKMIGRTPGNIVAIRPLRDGVISDYDVTEKMLKSFIDKIVDKKGFGRFFMPRIM
                     VCVPTGVTEVEKRAVEDATRQAGAREVYIIEEPIAAAIGAGVDISQPNGNMIIDIGGG
                     TVDIAVISLGGAVVSESIKVGGDRFDDAIVKYMKKQHNLLIGERTAEKIKFEIGSAFK
                     REEEKYMKITGRNLITGLPSSITINSTEMLEALRECVEQIVVATHAVLEKTPPELAAD
                     IGDVGIIMTGGGSLLYGLDKIIEQRTGITVTIADEPLSCVAKGTGIALGSIDLLETGG
                     SFKRK"
     gene            complement(25979..26239)
                     /gene="spoIIID"
                     /locus_tag="PC700_000443"
     CDS             complement(25979..26239)
                     /gene="spoIIID"
                     /locus_tag="PC700_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HDF2579769.1"
                     /translation="MRSHIEERAIVVAKYILEKNTTVRQTAKTFGVSKSTIHKDVTER
                     LKEINPSLAKEVKNVLDKNKSERHIRGGLATKLKYEKEHKKM"
     gene            complement(26337..27005)
                     /locus_tag="PC700_000444"
     CDS             complement(26337..27005)
                     /locus_tag="PC700_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HDF2579770.1"
                     /translation="MKKKLLEKDGFYLSLFVCVCLLAVGGVWFTNNNVDKLASNKGIM
                     ENANKDSEEEIHLIEKDKKDAIPTATDSKQNLEKAKSKEENKSSTTKLNYIGDKVIRG
                     YSEKEPSYSKTLDVWETHKGVDISCTKGKEVKSLLNGIVVDVFNDEEYGQSVKIKSDN
                     NIVVVYSNLDKNVSVKKEQKVTEGQSLGTVGSTSQIESEEGIHVHLEAYSGEKSIDPM
                     SLIK"
     gene            complement(27071..28135)
                     /gene="spoIID"
                     /locus_tag="PC700_000445"
     CDS             complement(27071..28135)
                     /gene="spoIID"
                     /locus_tag="PC700_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HDF2579771.1"
                     /translation="MKNPLVVLLGFVTCSVLVPSLITLVSYKNVELTEKPESPVSINK
                     TIKKSDIEDKGNKEEKSTVSYETVNKKAPIINVYNHITGKTEKMDMENYLCGVLAGEM
                     SSEFDIEALKAQSVAARTYVVYKQEHGKSSKHKNAVVCTDYKHCQEYKSYDTLKKLNG
                     EEWIKNKYSKIQEAVRGTKGQIITYNDKAILPLYFSTSSGKTENSEEVFSAKYPYLKS
                     VESPYDKYSPKFASTLKISNTDFVKSLRRAYSTIVIDVNNLSKQVSITKRSDAGTVEK
                     IKLGNKELTGKDIRTVFKLNSANFDIKFGEGYIDFVVKGYGHGVGMSQWGAEGMAEEG
                     YKYYDILSHYYTDTKIKDIY"
     gene            complement(28222..29475)
                     /gene="murA"
                     /locus_tag="PC700_000446"
     CDS             complement(28222..29475)
                     /gene="murA"
                     /locus_tag="PC700_000446"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDF2579772.1"
                     /translation="MAKIIVKKSNPLKGSVKIDGAKNAVLPIIAATLLANGKSTLNGV
                     PNLRDVHVISDLLRHVGAEVEYKENTLTVDASNIKTCEAPYELVRKMRASFLVMGPLL
                     ARFNSTKISMPGGCAIGTRPIDLHLKGFKALGAKIEMDHGFVEAATEKLVGNKLYLDF
                     PSVGATENIMMAASLAEGTTIIENAAEEPEIVDLANFLNEMGADVKGAGTNTIKIKGV
                     KELKGAEHNVIPDRIEAATYMVAAAMTKGDITVENVLMEHLKPVVAKLREAGCEITEM
                     DNSVRVVGPKVLKPIDIKTLPHPGFPTDVQAQFMAMLTVANGTGVVIETVFENRFMHV
                     AEFNRMGANIKIDGRSAVVNGVDELHGAAVNATDLRAGAALILCGLIAEGETQIGEIY
                     HIQRGYVDIDKKITALGGQIEIVED"
     gene            complement(29496..30233)
                     /locus_tag="PC700_000447"
     CDS             complement(29496..30233)
                     /locus_tag="PC700_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HDF2579773.1"
                     /translation="MKKVKLAISFFILMIIGMTISYADTSINTRYNRLSKALEISEAN
                     FKFYNVKINSVINHNLSNKEFHNIFIEIINNLKLDKKGINWNQKGSGDKIQLCATIKG
                     SDKEISIKGINKNNKESYIVVDILDNKVYKHKEVIYSNVENVLNKYSSQVDVSACIVG
                     EYTKRLQNNKYDDILQKILYNMSAKQIDEVKDVNFLSITAYSKVLNDYELEYLGEKIN
                     LNIGIKYNEDDGKTLIYIATPIIKLDY"
     gene            complement(30703..31998)
                     /locus_tag="PC700_000448"
     CDS             complement(30703..31998)
                     /locus_tag="PC700_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase"
                     /protein_id="HDF2579774.1"
                     /translation="MTNDFKKEKKEDYFVNLKAISTEVLQEKMQDNAWVIVDTRLNDA
                     YNGWKLDGVKRGGHIKRAVDFSANWLNVYSDRKDEVLEQALKTKRIDLDKNIVLYDAN
                     GKDALVVADYLSKKGYKYLYKYDIKQWADDENLPMERYKNYQMIVPAFIIKDILDGKI
                     PETFEDSKNIKMIEASWGEESYTKGHIPTSVHVNTDIIEPPPTWMLDNDDNLTKFALD
                     YGLTKDDTVIVSSITPMASYRLAVILRYIGVKDVRVLNGGTNSWLSAGYELEFISNPK
                     HSCTNFGADIPVNSQLVVTTSELRQKLKEKNKFILVDNRTWDEHIGKVSGYTYYDKKG
                     RIPGALYGHSGSNSVSLEEYRNIDNTMRNKSEILEMWDKENIDVNKQLIFMCGSGWRA
                     AEVLTYANVIGVENTSLYSDGWMGWSLDNSNLIEVGEHK"
     gene            complement(32451..34283)
                     /gene="glmS"
                     /locus_tag="PC700_000449"
     CDS             complement(32451..34283)
                     /gene="glmS"
                     /locus_tag="PC700_000449"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDF2579775.1"
                     /translation="MCGIVGYLGSRKAAEVIVEGLSKLEYRGYDSAGVAVNSSNEEEL
                     NIRKFKGRLSVLAEDLEKNPIDGNLGIGHTRWATHGEPSDVNSHPHFNQAKTIAVVHN
                     GIIENYMEIKEELISEGVKFESQTDTEVIAHLVDKYYEGNLLDAVYKTISKLRGAYAL
                     GVICKEHGNELVAVRKDSPLVVGVGEGENFIASDIPALLKYTRDVYFLENGEVVHLKD
                     ENVTVYDSNRNLVEKEVFHVTWDVEAASKGGYDYFMSKEIHEQPTGVRETLERRLDDN
                     GNIILDSINISKEDLEKINKVYIVACGTAYNAGLLGKYAIEKFVNIPVITDIASEFRY
                     SDPFVDENSLVILVSQSGETADTLAVLRDSKAKGARILSITNVVGSSIARESDDVFYT
                     WAGPEVAVASTKAYTTQITSLYMIALDFAIKKGTITREFYDSMISKMKEIPSKIQEIL
                     DNEEYIKEVAKTVVSSEHAFYLGRGIDYSLAMEGSLKLKEISYIHAEAFAAGELKHGT
                     IALIEKGTPVIAIATQEKLFEKMVSNMEEVRARGAYVVAIAQSHNKDVEKAADKIIYI
                     PNSDDILSPILAVVPMQLLAYHVSVLRGCDVDKPRNLAKSVTVE"
     gene            complement(34550..35896)
                     /locus_tag="PC700_000450"
     CDS             complement(34550..35896)
                     /locus_tag="PC700_000450"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDF2579776.1"
                     /translation="MRKYFGTDGVRGVANTELTCDLAYKLGRAGGFVLAQGDHRVKVV
                     VGKDTRISGDMLEASLIAGLMSVGCDVITVGIIPTPAVAYLTRKYGADCGVVISASHN
                     PVEYNGIKFFNKNGYKLDDEIELKIEEYIDDIDKIDCLPIGENVGRKLHEHCAQRDYV
                     DYLKSIISTDFKGLKVVLDCANGASYKVAPIVFDELGASVISINSSPDGNNINYKCGS
                     THPEQLQRAVLEHNADLGLAYDGDADRLIAVNEKGQIVDGDHIMILSALNLKKNNKLA
                     QDTLVVTVMSNIGLTIAAKENGINLSTTAVGDRYVLEDMVKNGYNLGGEQSGHMIFLD
                     YNTTGDGVLSSLILANIILQEKKPLSEIASIMSQYPQVLVNATIKNENKNKYMEYPEI
                     KTEIERIESILNGNGRVLIRPSGTEPLVRVMLEGKEEGQIKELATNLANLIQEKLS"
     gene            complement(36066..36623)
                     /locus_tag="PC700_000451"
     CDS             complement(36066..36623)
                     /locus_tag="PC700_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HDF2579777.1"
                     /translation="MSTARVICAGFGGQGVMSMGQLLTYAGMLEGKEVSWLPSYGPEM
                     RGGTANCAVTVSNEPVGSPLITDDATAAILLNIPAFEKFKDDVIPGGKIIVNSSLIKE
                     KVDRTDVDVYYIPANELAAELGNDKVANMIMLGAYLKVSDTVDIESVLEAFKKVFGPR
                     KEKFVPLNREALQKGMDATCSKATN"
     gene            complement(36624..37376)
                     /locus_tag="PC700_000452"
     CDS             complement(36624..37376)
                     /locus_tag="PC700_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HDF2579778.1"
                     /translation="MAVVFKKTEGLQDTQTHYCPGCTHGIIHRLVGEVLEELGVLGDA
                     VGVVPVGCSVLGYKYFNCDTQEAAHGRAPAAATGIKRVHPENTVFTYQGDGDLASIGT
                     AEIVHAAARGEKITTIFVNNTTYGMTGGQMAPTTLVGQRATTAQSGRNAETQGYPIRV
                     SEMLATLTGAVFVERVAVDTPAHVRQAKKAIKKAFQVQQAGLGFGIVEVLSTCPTNWG
                     LAPNDALQWLRDNMIPYYPLGNFKNVEVEEVK"
     gene            complement(37376..38455)
                     /locus_tag="PC700_000453"
     CDS             complement(37376..38455)
                     /locus_tag="PC700_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HDF2579779.1"
                     /translation="MAKILMKGNEAFGKAAIEAGCKYFFGYPITPQSELPEYLSRELP
                     KIGGAFVQAESEVSAINMVYGGAGAGARVMTSSSSPGVALKQEGITYAVGAEVPCVVL
                     NVMRGGPGLGSIQPSQADYFMSTRGGGNGDYRTPVFAPATVQEAVDMIMEAFDVADYY
                     RSPVMVVADGMIGQMMEPVEFRAPEKKRELPPKDWATVGTKGKRKPNVINSLYLEPEV
                     LEDHCWHLQEKFDAMEKNEVQYEMYKTEDAEFVFAAYGTTSRVVKSAIDILREEGIKA
                     GLIRPKVLWPFPFEAFNQIPNARNILTVEMSMGQMVEDVKMAVEGKLPVYFHGRPGGM
                     TPTPAEIVEKAKKIIAGELVAGGAR"
     gene            complement(38484..38699)
                     /locus_tag="PC700_000454"
     CDS             complement(38484..38699)
                     /locus_tag="PC700_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2579780.1"
                     /translation="MAKGKVSFNQERCKGCGLCVEACPVKIIQLDSNVINKKGYNPAT
                     VFEMEKCIGCASCATMCPDVVITVERD"
     gene            complement(38742..39419)
                     /locus_tag="PC700_000455"
     CDS             complement(38742..39419)
                     /locus_tag="PC700_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2579781.1"
                     /translation="MITNDKRIRIITGHYGSGKSEFAMNYVVKLRDMVSGKVAIADLD
                     VVNVYFRTREKKELMKSLGIQPIDSSINAPTLDLPAVSAEVMSPMVDHSYNSVIDLGG
                     DNVGARVIGRFSHLLKEGDYDMLFVINANREKTQTSEEVIQYIKEIEKSSKLKVTGLI
                     NNTHLIRFTTIDDVLRGQKVAKEVSEKCNIPIRYVACLENLVEQLPKDLEGEIFPIKL
                     YMREDWM"
     gene            complement(39409..40488)
                     /gene="buk"
                     /locus_tag="PC700_000456"
     CDS             complement(39409..40488)
                     /gene="buk"
                     /locus_tag="PC700_000456"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HDF2579782.1"
                     /translation="MSKIFKILTINPGSTSTKIAVFDNEDLVFEKTLRHSSEEIGKYE
                     KVSDQFEFRKQVIEEALKEGGVKTSELDAVVGRGGLLKPIKGGTYSVSAAMIEDLKVG
                     VLGEHASNLGGIIAKQIGEEVNVPSYIVDPVVVDELEDVARISGMPEISRASVVHALN
                     QKAIARRYAREINKKYEDINLIVAHMGGGVSVGAHKNGKIVDVANALDGEGPFSPERS
                     GGLPVGALVKMCFSGKYTQDEIKKKIKGNGGLVAYLNTNDAREVEERIEAGDEKAKLV
                     YEAMAYQISKEIGASAAVLKGDVKAILLTGGIAYSKMFTEMIADRVKFIADVKVYPGE
                     DEMIALAQGGLRVLTGEEEAQVYDN"
     gene            complement(40510..41412)
                     /gene="ptb"
                     /locus_tag="PC700_000457"
     CDS             complement(40510..41412)
                     /gene="ptb"
                     /locus_tag="PC700_000457"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HDF2579783.1"
                     /translation="MRSFEEVIKFAKERGPKTISVACCQDKEVLMAVEMARKEKIANA
                     ILVGDIEKTKEIAKSIDMDIENYELIDIKDLAEASLKSVELVSQGKADMVMKGLVDTS
                     IILKAVLNKEVGLRTGNVLSHVAVFDVEGYDRLFFVTDAAMNLAPDTNTKKQIIENAC
                     TVAHSLDISEPKVAAICAKEKVNPKMKDTVEAKELEEMYERGEIKGCMVGGPFAIDNA
                     VSLEAAKHKGINHPVAGRADILLAPDIEGGNILYKALVFFSKSKNAGVIVGAKAPIIL
                     TSRADSEETKLNSIALGVLMAAKA"
     gene            complement(41716..42897)
                     /locus_tag="PC700_000458"
     CDS             complement(41716..42897)
                     /locus_tag="PC700_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2579784.1"
                     /translation="MINRLKNNTKIKIISFLSAIALWMYVMAVVDPEETKGFEDIPVS
                     ISNMSDLKDKNLVIYPDEKLTADVYIKGSLSKVQSVKKEDIHVYGTIEDPIQGQKEVY
                     LKANIPQGVTLEFKHDVLVVNLEKNIKEKRDIKVEVSGNSKRNINKIELSKEFVDVSG
                     PRTLVKEVKSVRANLDVGNETDDFSKKLQLIPLNSDGTKVEGVTLSSSFVTAKIELLK
                     EKIVPIRVSLNDNKDNDVLKNYKLSKEQVTIKGKKEDIDKISYIKTKPIDLADLANGE
                     EKEVSLEIPEGITLEDSSITIKLNDTKQLSAEFLYNAGDIELRNIPTNIDNPNVISDV
                     DIKVVVESTEDLSKLNKKDISLYIDLSQGPEEDSKYKIKYETTYQFKKINIEPNIVEV
                     E"
     gene            complement(42894..43733)
                     /locus_tag="PC700_000459"
     CDS             complement(42894..43733)
                     /locus_tag="PC700_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HDF2579785.1"
                     /translation="MLDINSFLNGFFNIILRMSIYDLIDISIVAYIFYKIFMFIKDTR
                     AEQVFKGIIFLLLATQLSNTFKLHTVYWISLKALDYGVIAALIIFQPEFRAGLEHIGR
                     AKFNLFGKNVNTSEETLNRNIEEIVEALYSLSRQKIGALIIMERETRISDIINTGTII
                     DAEISRQLLINIFIPNTPLHDGAVVIRDSKVKAAACFLPLTESKDLSKDLGTRHRAGI
                     GVSEVSDCITLIVSEETGGVSIAKAGKLYRDISRERMMNILRSNLKTNTETRSFFKGG
                     IFK"
     gene            complement(44302..45834)
                     /locus_tag="PC700_000460"
     CDS             complement(44302..45834)
                     /locus_tag="PC700_000460"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003228330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HDF2579786.1"
                     /translation="MMKKPVALIIMDGFGYNKDVKGNAIAESKTPNLDRIKKEYPNTL
                     INASGLDVGLPDGQMGNSEVGHTNIGAGRIVYQDLTRITKSIKDGDFFANKVLCEAMD
                     NAKENSLHVMGLLSDGGVHSHIDHLKAIIKMAKDKGVQKVYVHAFTDGRDTDPQSALE
                     YAKEVQASMDEIGVGEFATVSGRYYAMDRDKRWERVELAYNAMVRGIGEKANSIEEAI
                     QNSYDDGKNDEFIMPTVIMKDDKPVGSIKENDSIIFFNFRPDRARQITRALVCEEFDG
                     FKREDIKNFFVCLTEYDITIENVHIAFGPQSLANTLGEYLAKNGKTQLRAAETEKYAH
                     VTFFFNGGVEEPNKGEERLLIPSPKVATYDLKPEMSAYELTDKALDKLGEDKFDFIVL
                     NFANPDMVGHTGSIEAAIKAVETVDTCVGKLIDKIVELGGSAIITADHGNAEYMLDPE
                     TGKTVTAHSINPVPFIVVGQEYESAKLLDGGRLSDIAPTVLDMMKLEKPEEMTGHSLI
                     SK"
     gene            complement(45843..46586)
                     /locus_tag="PC700_000461"
     CDS             complement(45843..46586)
                     /locus_tag="PC700_000461"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDF2579787.1"
                     /translation="MRKPIIAGNWKMHKTIKEALEFVNEVKDKVNSDKVEAVICAPFT
                     LLKDLKEATKGTNIKIGAQNMHFEEKGAFTGEVSPLMLKEIDMDYVVIGHSERRQYFN
                     ETDETVNKKVLKALEVGIDPILCVGETLEQREAGKTKDVCKVQVEKALENVLKDDLSK
                     VVVAYEPIWAIGTGKTATAEDANDVISYIREVIKGLYGELANEVRIQYGGSVKPSNVA
                     EIMGQSDIDGALVGGASLASNDYLDLVNF"
     gene            complement(46612..47814)
                     /locus_tag="PC700_000462"
     CDS             complement(46612..47814)
                     /locus_tag="PC700_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDF2579788.1"
                     /translation="MSMLNKKTIEDIDVCGKKVLVRCDFNVPLQDGVITDENRLNGAL
                     PTIQYLISKGAKVILCSHLGKPKGEAKPELSLAPVAKRLSEMLGKEVVFAADDNVVGE
                     NAKKATEKMENGDVVLLENTRYRKEETKNEENFSKELASLAEIFVNDAFGTAHRAHCS
                     TVGAGEFLQERVCGYLIQKELKFLGEAVANPVRPFTAILGGAKVSDKLAVINELLEKV
                     DNLIIGGGMAYTFLKAQGYEVGTSLLEIDKVEYAKEMMEKANNKGVNLLLPVDVVMAD
                     HFAPDATPIVTEDANVKEDYMGLDMGPKTIANFVKTIKESKTVVWNGPMGVFEFENFA
                     NGTLSVARAMAELTDATTVIGGGDSAAAVNQLGFGDKMTHVSTGGGASLEFLEGKELP
                     GIAALDNK"
     gene            complement(48036..49043)
                     /gene="gap"
                     /locus_tag="PC700_000463"
     CDS             complement(48036..49043)
                     /gene="gap"
                     /locus_tag="PC700_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDF2579789.1"
                     /translation="MVKVAINGFGRIGRLALRKMMEQQDKFEVVAINDLTDAKMLAHL
                     FKYDTAQGRFNGEIEVKEGAFVVNGKEIKVTAERNPADLPWAELGVDIVLECTGFFTS
                     KDKAEAHIQAGAKKVVISAPATGDLKTIVFNTNSDILDGSETVISGASCTTNCLAPMA
                     KVLNDKYGIEKGLMTTIHAYTNDQNTLDGPHPKGDLRRARAAAGNIVPNTTGAAKAIG
                     LVIPSLKGKLDGAAQRVPVVTGSITELVCTLGKNVTVEEINAAMKEASNESFGYTEEM
                     LVSSDIIGISYGSLFDATQTKVMEVDGKQLVKVVSWYDNEMSYTSQLIRTLGYFAQLA
                     K"
     gene            complement(49103..50125)
                     /locus_tag="PC700_000464"
     CDS             complement(49103..50125)
                     /locus_tag="PC700_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2579790.1"
                     /translation="MKNLLEIQKKLIPQAIELMERRYSILRQISLSEPIGRRTLSNVL
                     EISERIIRSETEFLKEQGLIDVAVSGMTITKEGSDLLDKLKDIMSDIMGLSKLQDRVR
                     EKLGIKRVILVPGSCDENGSLLKDVTRYGCEYFLGILKDGDIVSITGGSTMLEFSNVI
                     KTDKRYPNSTIVPARGSMGTDVEMQSNNIVASISKKLHSNCKLLHIPDELDESAMKTL
                     SQVPEIKTTLEYIKRTDILVFSIGRADVMAKRRKLPEDKVDEILSKNAVGEAFGYYFN
                     KDGEIVYKLNTVGIDLETVKQVKETIAIFAGTEKVEALLAISNMHKNMVLVTDEESAY
                     KILSLL"
BASE COUNT        14859 a         9669 c         5448 g        20351 t
ORIGIN      
        1 tcaatcacaa tataggactg aagttataaa taaattatgg ctattcaata catttaattt
       61 tatattttct tgtctaatta tttctttatt atatttccca tttttttata aagtggcaat
      121 gttactaaag atattataac agccttcagc acattaaatg gagctatcat ccaaattaca
      181 aaagtgaata aatcatgaac ttgaggattt atagctgaac ctaaacctat tatagcatct
      241 agtcccataa gttgaccata aagtggcaac attacaaaat aatttactaa tgaaccaatt
      301 atagtcatag atattgttcc taatacaaaa ccaatcagta caccttttaa atctccactt
      361 ctcttatatg aataacaaag tatcattaca taagaaccac caattaatat atttgcaaat
      421 tctcctattc ctccagttgt tgttgctgtt attgcttgta acacattttt tactacaact
      481 attccaaatc caactaatgg tcctagagac attcctccga atattgctgg aatatctgat
      541 aagtcaattt ttaagaaatc tggaaatata cctggtattg gtgcttgaat aaacattaat
      601 aaataagata ttgcagataa aattgacatt actactaaag ttcttgttga aatcatattg
      661 cctctttttg ctgtgttttg cataattatt ttccccctaa tcaatatgtt tatatttttt
      721 aggaaaaaca aaaaactcga agaccctatc ttcgagtttt tatggatata gtttaaacaa
      781 actaaaataa acttatttat cttccataaa tatcttctct catccagact ttactgtcgg
      841 ctttggaatc tcaccaaatc aaccacctaa gtagttcgcg ggctataccg ccggtaggga
      901 atttcaccct gccccgaaga tcttctttga ttttttaatt ttccttacac tcttatatta
      961 gttaattttt taataattgt caagttattt tttctcatac actactttgc catttattat
     1021 agtgtaaagt acattacttt gaatctctag tggagaatta tcccaaatta ctaggtctgc
     1081 atctttacca acttctatag agcctactct atcttctatt cctaaagtct tagctggatt
     1141 tatcgtaatt gattctattg ctttttcttc tttcatacca tgttttactg caatacctgc
     1201 acatattgga agatattgaa ctggtattac tggatggtct gtcattatac atacatcaag
     1261 acctgcattt gaaagtattc ctgctgtatt aaatgtcaaa tttctaagct caaatttaga
     1321 cctttctgac aaagatggcc ctactattac aggaaaccct tcttccacta attcatctac
     1381 aataagatga ccttctgtac aatggtctaa tgtaagcttt aaattaaatt cttttgcaat
     1441 tcttattgct gtaaacatat catctgctct atgagcatgt gccttaaaag gaatttctct
     1501 tcttaaaact ggtattaaac tttccatttt tatatcatat tcaggttttt cacagtcttc
     1561 atcatcatga ctctcataca tatcaatatc ctctaaatat tcttctgctt tttttaggtt
     1621 ttctctaagt aaagcagcta tagccattct agtttgagga gatttatcat cctgaccata
     1681 acagctcttt ggattttctc caaaagcaat tttagatgca actggatttt ttattaccat
     1741 tttatcaatt ctttttccac atgtctttat agctatacat tgaccaccca taacatttgc
     1801 acttccaggg gttgtacaaa cagaagttat tccaccttca aaagcttctt taaaagttct
     1861 atccattgga ttaattccat ctataggatt taattgtggt gttattgggt cagtctcttc
     1921 atttccatct gctccttcaa accctatacc atcttcccat aaacctaagt gagtatgagc
     1981 atctataaat ccaggaaaca caagtttatt actagcatct attacctcta catctaaagg
     2041 agctatcaaa tcttctccaa tttctattat ttttccttca tctataagta tatctccatg
     2101 aattatccca tttgttatgg tatttatttt accatttttt ataaaaatca ttcattttcc
     2161 tcctgatttt caagactatc gttcttattt taatatattt tcttactata tacttttaca
     2221 aatttttaac atttatacac tcgagtaatc tcatttacaa atatagaatt taagtactat
     2281 aaccatacat aaacttatct attttttcat tgataaggca actctttttt tgtttaagtc
     2341 aattccaatg acttttacat ctactatatc acctactgta acaatactca tagggtcttt
     2401 cacaaaacta ttactcattt ctgatttatg aactaatccg tcatttttta ttccaatatc
     2461 tacaaatgca ccaaagtcta caacatttct tattgtacct tttaaagtca taccctcttg
     2521 tatatcttca atttttaaga catcagttct aagtataggt tttatacctt cttctcttgg
     2581 gtctcttcct ggttttttga tttcagctat gatatctttt aacgtaactt gtcctacttc
     2641 cagtttttca cttaattccc tcaagccaat ttcttttatt ttttcatcta tttcacctat
     2701 attcttattc tttacatcat ctaaagaata tccaattatc tcaatcattt ttttacatat
     2761 atcataactt tcaggatgca cacctgtatt atctagtgga tttttaccct ctaagattct
     2821 cataaatcca gcacattgag taaatgcttg aggtcctagt ctttttactt ttttcaattg
     2881 tgctcttgat gtaaaatctc cattttcttc tctatatgct attatatttt ttgctatagc
     2941 tttacttatt cctgcaacat gttctagtag ggagtatgat gcggtattta aatctactcc
     3001 aacactattt acagaatctt caacaactcc atctagcact tcttctaatc tctttttgtt
     3061 taaatcatgc tgatattgac caacaccaat acttttaggg tctattttta caagctctgc
     3121 taatgggtct tgtaatcttc tagctataga tatagctcct cttattgata catttatatc
     3181 tggatgttcc tcatttgcaa gttcagatgc tgaataaaca gatgctcctg cttcactaac
     3241 tatcacatac tgcacttcgc tatctatttc ttttatcatt tctgatacaa aagtttctga
     3301 ttctctagat gcagtaccat ttccaattga aattatatct atatcgtatt tttcaatcaa
     3361 tccttttaaa actttctttg ctccctctac atcattttga gggtctgttg gatatactgt
     3421 tgtataatct agtagtttac catttttatc tactacagct attttacatc cagttctaaa
     3481 tgcagggtca aatcccataa ctactttgtc tttaactggt ggttgaagca ataaactttt
     3541 aacattcttt ccaaatacac ttatagctct ttcttgagct atttctgtta aatgatttct
     3601 tatttctctt tctattgatg ggaaaattaa tcttttataa gaatcttcta ttgaacttac
     3661 gatttcttct ttatttttga aatttttatc atttacatat tcgtttatta tgtaatttaa
     3721 aactttatca ttatttattt ctaacttaac ctttaggaag ctttcttttt ctcctctgtt
     3781 tattgccaaa actctatgtg gagccatact ttttactgct tcgttataat cataatacat
     3841 atcataaacc gatttctcat cagtagcact tttgctaact atcatacctt ctcttaaagc
     3901 taattctctt atatactttc taatcttagc atcatctgag actagttctg caattatatc
     3961 tctagcacct tttagagcat cttcaataga aagaacttct ttctcttcat caagataatt
     4021 ctttgcctct atttctatat tatctaaatt attctctagt attgacaatg ctaaattttc
     4081 taatcctttt tcttttgcta ttgttgctct agttcttttc ttttgtttat atggagcata
     4141 tatatcttct acttcttgta atgtttttgc tttttctata ttttgtgtta tttcatctgt
     4201 taatttccct tgttcatcta ttagtctaac tacatcatct tttctacttt gaagatttct
     4261 caaatacatt aatttttcat aaaattctct tagtgtaaca tcactcattt cgccagtcat
     4321 ttcttttcta tatctagcta taaatggtat tgtatttcct tcatcaataa gttttaatgt
     4381 attatttatt tgttcatctc taagattaaa ctcttttttt aagatttgat ttatatccaa
     4441 tttattcact cctaatgtta gtttttcata agtataacaa aacagaacga aggctgaggg
     4501 ttatcgtcct cagcctaagt gctgtaagtg catattaagc actaaccttc ttatacatat
     4561 atcttaatta cagtgcatat ttattaaact attttattca tttttaagta ttcattaata
     4621 aacaagtcta tatttccatt cattacacta tctacatttc caaactcagc atttgttcta
     4681 tggtccttaa ccaatttata tggttgaaaa acatatgacc taatttgact accccaagtt
     4741 atttgtgagt atttgccttg tatatcttct attttttcct tttgctctaa ctcttttaat
     4801 tctatcaatt tcgccattaa aagtctcata gctctgtcct tatttagatg ctgagacctt
     4861 tcattttggc actgaactac tacaccagta gggatatgtg ttattctaac tgctgaatca
     4921 gtagtattta catgttgccc tccagctcca gaagctctat atgtgtctat ctttaaatct
     4981 gacggattaa tttctacttc tatattttca tctaactctg gtgttacatc tatagatgca
     5041 aatgaagtat gtcttttccc tgatgggtca aatggagata ttctcactaa tctatgaact
     5101 cctttttcac tctttaaata cccataagca tttgttcctt ctacaagaat tgtagctgtc
     5161 tttatccctg cttcagggtc tgatattata tctaaaagct taactttata acccttactt
     5221 tctgaccatc ttatatacat tctcaaaagc atttgagccc aatcttgagc atctaagcca
     5281 ccagtaccag catttataga taatatagca ttatttttat catactctcc atttagaaga
     5341 gtctttattt tcatttcaga taatttttct tccattgatt ctatggactt ttctatatct
     5401 ctttctacag aatcatcatc ttcttctaaa cctatttcaa ttaatacttc tatatcttct
     5461 aataagcttt ttaagctttc atattcttct aatgtctctt ttaaagattt attttcttga
     5521 agtactcttt gagcgacttc attatcattc caaaagtctt gttgattcat cttttcttca
     5581 ttaatttgta tatttttcat caatgaagct atgtcaaaga gaaaccctca attcttctaa
     5641 atttgaagcc atctgctcca tactatgtct atactcttgt aactttaaca tttaatcctc
     5701 cgagattatc ttccacaaca gtttttatat tttttaccac ttccacaagg gcataagtca
     5761 tttcttccaa ccttttcatc ttttttaaca gttttacttg ttggaagtgt tccatctgat
     5821 ggagaagata gattttcaac atctacaact gcttttcttt ccactggagt ttctattgtt
     5881 atattaaata aatatcttac agtgtcttct ttgatatgtt tattcatttc atcaaacata
     5941 tcaaaacctt ccattttata agcaataact gggtcttgct gacctacagc acgaagacca
     6001 ataccttggc gtaattggtc catagcatct atatggtcta tccaatggtt atcaacagct
     6061 tgaagtaaga ttactctctc aacttctctc attctctcat agccaacttg ttcttctttt
     6121 gaagtatata tcttcatagc tatttcataa actttttctg ttatttcaac tggattaagt
     6181 ttttctattt ctggtatttc tatgctacct tttggcaaga acagattata catatgttct
     6241 ttgaatcctt cttcatcaaa tcctttatct tgtgtataaa gagtaactgc ttcttctatt
     6301 atagaatgag tcattgattg tatttgttct tgtaaatctt caccttctaa tacacgtttt
     6361 ctctctgcat atattatttc tctttgttta ttcataacat catcatattg aagtacatgc
     6421 tttcttattc caaagttttt accttcgact ttcttttgtg ctccttcaat agacttagat
     6481 aatattctat gttctatcgg catgtcttct tcaagaccta ttttatctac aataccagat
     6541 attctatcac taccaaacaa tctcataagg tcatcatcaa gacctatata gaatcttgaa
     6601 ctccctgggt caccttgacg accagcacga cctctcaact ggttatctat tcttcgagat
     6661 tcgtgtcttt ctgtacctat tatagcaagt ccacctgctt ctacaacttg tttttgttct
     6721 tcttgtgttt gttgcttaaa cttttcaaat agtttttcat actcttctct agcttcaaat
     6781 agaatttcat taccttttac aggtatacct tctattggag tatctaccct atttactatt
     6841 tcctctttaa atccatttct tctcatttct ctcttagtta agaaatctgg gtttccacct
     6901 aaaactatat ctgtaccacg gccagccata tttgtagcta ttgtaacagc gcctaatcta
     6961 ccagcctgtg ctattatctc tgcctcttta tcatgatgct tagcatttaa tacttcatgt
     7021 ttaatgcctt ttttctttaa tatttgtgat aataattctg atttttctat cgatactgta
     7081 cctacaagta ttggctgatt aactttatgt ctttcaatta tttcttgtgc tacagcatta
     7141 aatttaccta tttcactttt gtatacagaa tctggtaaat cttctcttac cataagctta
     7201 ttggtaggta cttggaaaac gtccatttta taaattgctt taaactcttc ttcttctgtt
     7261 tttgctgtac cagtcatacc agaaagtttt ttatacattc taaagtagtt ttggaatgtt
     7321 acagtagcta gtgttttaga ttctctttgt attttaagac cttctttagc ctctattgct
     7381 tgatgtaaac cttctgaata tcttctacca aacataagtc tacctgtaaa ctcatctact
     7441 attactattt ctccatcttt agcaacataa tcaacatctt tcttcattat tacatgtgct
     7501 cttaaagctt gatttatatg atggtataat tctgtatgag atatatctgt tatattatca
     7561 acattaaaat acacttctgc tttttgtata cccgatgctg ttagagatac agccttatct
     7621 ttttcttcta attcataatc atctggtttt aaggttaaaa caaatgtgtt tgcatctgaa
     7681 tataagtgag ttgatttgtc tccaggtcca gatatgataa gtggtgttct agcctcatct
     7741 attagtatag agtcaacctc atccactata gcataattta atcctctttg aactctttgc
     7801 tctttgtgta ttaccatatt gtcctttaga tagtcaaatc catattcatt atttgtacca
     7861 taagttatat cacaatcata ttgttctttt ctaacttttg gattttgacc gtgtataata
     7921 acgcctacac tcattcctaa aaactcatag atttttgcca tttggtctct atcacgtttt
     7981 gcaaggtaat catttactgt tacaacatgt acaccttttc cagtaagcgc attcaaataa
     8041 acaggagcag ttgcaaccaa agttttacct tcacctgttt tcatttctgc tattcttcct
     8101 tgatgtagta ctatacctcc aatcatttga actctgtagt gtctaagtcc taacactctc
     8161 ttagaaactt ctctaactac tgcaaaagct tctggaagta tatcatctat actttcacca
     8221 tttgctaatc tttctttaaa tatatttgtc atatttttaa gttccgagtc tgccatagat
     8281 tcaaatttag gttctaatga atctattata tcaacagttt tattgaactt ttttaattct
     8341 tttttatctg ccatgttgaa taaattatcc ataaagctca tattttacat ctctcctcgt
     8401 tctttattgt actattattc attttaccat aaaaactata cttttaatat taatttaaaa
     8461 agctatcgat aatttattta tcgatagcta atatgattaa atctaaattg ctttattcat
     8521 gttctattat accatatcca ccattattac gcttataaac tactgctatt tcatctgaat
     8581 ctatgttctt gaacatataa aaatcatgtc ctattaattc catctgtaat acagcttctt
     8641 cttcactcat tggcttaaca ctaaatttct tacgtctttg aattactaat tcttgatttt
     8701 catccccata atcctcttgt tcatcagcta gatttatctc tgaatcttgg aatcttatac
     8761 tcttgttgtc tttatgttta tcttgtaatc tctttttata tttttttaat tgtttactta
     8821 atttatcata tacaacatct atagctgtgt ataaattttc ctgcatatct tctgctctaa
     8881 ttataggacc atttacagta gctattgtta cttctatttt ttgtcttgct tttctcgcac
     8941 ttactgtaac ttttacatca gtgtctggat gaatgtaatt atctaactta cttagtttag
     9001 tttcgatttt attttttatt gcatcagtta attcgatttg ttttccagat ataattatgt
     9061 tcataaatac atctcccttc aattatgtca gtataagaaa atttatactt taataatata
     9121 ttctacacga actctcataa tcctcttttt attatttact tttatgattt tttctattca
     9181 attgatagaa ttatttatca tttttagtta cattgatact tatgtggatt aatattatta
     9241 caaacacctg tggataatgt ggataaaact gttaataact cttgtttttg tttatttaaa
     9301 gctattttta ctcctatttc tatccacaaa aatctccatt aattgtttat taatatgtga
     9361 attcatgtca taagatggta attttaggaa attatgtgta ttatatgcca atataccttt
     9421 ataatttttt aataactata aaccctctaa aacctccata atctatcttt tctttcatta
     9481 tttatattta aaactactta aaagttataa agcatactca acaataattt catatttaat
     9541 acctattaac attcatatac ccaaattata attaacttac tctctttatt taaactaagt
     9601 attttatatt catatactat cttgaaatta tatttacgag ttgtttaagc ttttgtctat
     9661 ctatatcttc aaaaggactg ttatttattc taatcgctgt accaaaatga aaattacttg
     9721 ctttagttaa ttcctttatc ttttctatat tattaaaatt aagacctcct cctaaaagaa
     9781 tttttatgtg attagaacta agcatcatat tctttatcat ttgtatgtta tcaactatac
     9841 ttcctttccc tcctgatgtt aatatattcg taattttatc ataatccttc aaaattttaa
     9901 cactatctat tgtatttgat tcatctatgg ctctatgaaa tgtaacatca agattatcac
     9961 aacatttcaa taaaacattt agatttttct catctatatt attgttttta tccaatacac
    10021 caaatacaac tccattagca ccaatctctt taaccacacc tatatctttt tgcattatac
    10081 ttatatcttc tttactgtat acaaaacttt tcgcatgatg tcttatcatt acgttcacag
    10141 gtatctttac actgttaaca acactttcta taagaccaaa acttggtgtc aatccacctt
    10201 cagttaaagc actaactaac tctattctat ctgcaccaca atcttctata attttagcgt
    10261 cttctactga cattcctatt atttctaaca taactactcc tttgctaatt catatattgc
    10321 atttgcaaat atctttgcat ttaatattat atcttctata tccatgtatt catcaggatt
    10381 atgagctatt cctttttgtc ctggaaatga aggtccaaac ggaactatat ttggcattac
    10441 ttttgcatac gtacctccat tagttgtcac gggagttcca tctaatgatg taaccctttc
    10501 ataagctagc tgaagctttt taacaagatt actctcttta tcaaaatata caggattaaa
    10561 attagaaatt acctctatat cgatactatt tggaaaacta gcttttattt catctaatac
    10621 atctttcaat ttataactaa aatcgtattt tacctttata tctaatatag tattactatt
    10681 ttctatattc agctctttat atgtaacaag cacattttca gacatatttt ctataaaacc
    10741 taggtataca tcttcttcat aattgatttt actttttatt ctaatctttg ccatgccttt
    10801 ttcaccatat acaacaggat atttacaatc tggtgtaaat cccataatag gtgttttttc
    10861 tttttctaaa taatatggta tatcttcaaa tccactttct tcatttgtac caaatattat
    10921 tctaactctc ttatctaatt ttaagtttaa ttctttaata gcatataatc catataaggc
    10981 agacattata ggtcctttat tgtctagtac tcctcgtcca taaattcttc cattagtttc
    11041 ttctccctta taaggctgat gcttccaacc atctccttca tgtacaacat ctacatgtcc
    11101 aattacacaa acataatcat cactatctcc atgctctgca taaccaatat aattatccaa
    11161 gtttctaacc ttaaatccca atttttttga tatttctaat gctttattaa gtgcctcccc
    11221 tactttttcg ccaaaaggga agccattttc tgactcccct ttgacactag gtatttttac
    11281 aatatctata atatcactta ataaatcttc ttttaactca tctattttat tacttatgat
    11341 attcataaaa atctccttat tataaaactc tattttaatt atgtaacact actttactca
    11401 cttacttcta tcggtgcttt tattctagcc tcatatgctt ccatccactc atttgatata
    11461 accttatgta tagtctttcc atctactcta tttgaaatat atacagcagg agattcttct
    11521 ttagttacta ctgaaaaagt gaaatcaact atatttgtag ctactcccca ttcaccttta
    11581 ttattcatgg caactaatga taaatcacca gcttttcctc ttcttttgat aagctgttta
    11641 tcaagttcat ttactgcaat atcacatgct tcttgtggat gctttccttc tttcataagc
    11701 ctaactatct cataagatat acatcccttc attaaatctt ctcccaaacc agtagctgta
    11761 gctcctccta tactactatc aacatagaat cctgaaccag atagtgcaga atctcctatt
    11821 ctacctcttt tcttcataaa caatccactt gttgatgtag ctgcacacat ttttccagtt
    11881 ttgtctaaag atatcatacc aacagtatca tgaccagcat atgggcttaa tcctttgtct
    11941 aaagtctctt tttttctctt tttataatgt aacttcgctc tatcagtcaa catatttttt
    12001 ctttcaaatc catttttatg agcataatct tcagcaccta ttccaactaa aaaattattt
    12061 accttctcat aacttaactt cctagctatg ctgacaggat tagaataatc tcttatacca
    12121 gcaactgcgc caatatctaa tgtgttacca tccataaatg cagcgtcaag ttctacctca
    12181 caattttcat ttggaagtcc accatatcca accgatttat agaatggata gtcttcaact
    12241 cttcttacag caagctctac agcatcacct gcttccatgc ctttttctaa atgttctgat
    12301 gcctcagtta ttccttctag ggccattctc catgtcgcta tcattcccca catgttaaaa
    12361 ttcccctcct taaattatat agattttact aatgtatgct tattataaat agtataatat
    12421 ttaaacttgc taataattaa tactcttatt aataacaaaa ttcttctttc atcaaagtta
    12481 tatcttcttt attcgctatt gcttttgcca atttacatat gttatttaat gaatcattaa
    12541 gaccaattcc accttttttg ggagtcttaa ctatacatat gtcattttta tattcgttta
    12601 tcacatctat attttcttct gacatagctg caaaagctgg tatatctgtt ttatcattta
    12661 tatttttaga cagtattgct gacatcttag aaaagttttc gtaattaaag cttggtccac
    12721 agattactac atcagggttt aattttttta ccatagctat catcttactc gttacttctt
    12781 tttcattatt tacaaaaaat tcatccccac aaaataaggt agctattacc ttagattcat
    12841 ttaaaaattg ctccatcatt acacctggtc ctaatgggct tgattttcca gcaggaggta
    12901 tattactttt ctcttttcct ccagctccag cttgtatttg atccaatatc ataactattt
    12961 tcttcataat cttcctccta taatactaaa tcatcaaaag atatatcatc atcttcctct
    13021 tcaacagttt cattttcttc tctcaagtat tgcttatcta gcatttttat aaatggtata
    13081 tacataaata ttcctagtat aagaagcact aattgtaaga ctgcaccttg ccatcctgtt
    13141 gttaaaaaac cagagaatat aattggagtt gtccacggta actgaactcc atttgtaaga
    13201 ggcactaatc ctgctgacat acagaagtaa gctattatta tatttattgt tggaactatt
    13261 gcaaatggta tcaatagtat tgggttaagc attattggta gaccaaatat caatggctca
    13321 tttataccaa atattcctgg taaaattgat agttttccca ttgactttat acgtttactt
    13381 ttacatatta ataacatagc tatgactaat gatagtgtac tacctgctcc accaaatgtc
    13441 gcaaacatat cttggaattg acctgttatt atgtttggta tttttgaacc agcctgaaaa
    13501 gcagctaagt tttcagctga tagaattttt aatattggat tatacacagc cccaacaaca
    13561 ctacttccat ttattccaaa gaaccagaat aaatgtaaga atatataagc gattgccata
    13621 gcacctaaac tatttcctaa ttttaataat ggcatttgta aaaacttaaa tataaattca
    13681 tgtatacttc catatggtgt agcttcaaaa gctatcttaa ctaaaaagaa aactaccata
    13741 actattgcac ttggtattaa tgctgcaaat gattttgata cggttggagg aactccatct
    13801 ggcattttca atgtccatcc tttttttaca actgcagcaa ataattttac agaggttatt
    13861 gctgttatca tacctacaaa cattccctta gagcccatcc aacctagtgg tatacctgtt
    13921 accatgtaga ctgattctgt tccttctggt gtatatggta ttacaaacgg tgttactata
    13981 aaaaaggcaa ctattgcaac tgcagctgat gctattggat ctacatctat ttgctttgca
    14041 taagaatatc caattcctaa aactgcaagt aaagtcatta cttcaaatgt tgctacagtt
    14101 ggttgttgca attttacagc ccaatctgga ccaaaaattt gacctacaaa ttcattccat
    14161 ccttttatag gaaaatttgc tactaataaa aataatgaac ctattattaa taatggtgat
    14221 gatattaaaa aaccatctct aattgcaatt aataatttat ttttacctat tttttcagcc
    14281 aatggcataa gtattctttc caatctattc atctatccac cctcatttcc tattattttt
    14341 tactataatt ctacgcttgt ttagatttta ttagctttat agcaagtttt aatacttttt
    14401 ccccattcat agttccataa tcaacttcat tgattatatc tataagtttt ccttgagctt
    14461 caaactttgg ttttaactcc tttagcatgt atttaacttg tggtcctaat aatatacaat
    14521 ctggattttt ttcttcaact attttgtcaa tttctgctat cggaaatgcc tctacttcta
    14581 ttggtaaatt gtgttcatct gctaccttct gcattttatc tgcaagtaaa cttgttgaca
    14641 taccaaagct acaaaataaa tatacttttc ttttcattct tttaaatccc ccttaatttt
    14701 tttattttaa tttttgaatt tcttttctta acataatcat ttcttttata aggtttctct
    14761 cactcataca agtcattaaa tggtcttgag agtgaatcaa taaaatagat atatctgatt
    14821 tatctccccc agcttccttt tgtataagtt cagtttgtac tttatgtgct tttaaaattt
    14881 cttcatttgc attttcaatt aattcatctg cttttttaaa atcgttttcc tttgcatagt
    14941 caagtgcttc ataaagcata gattttgcat ttccagcatg aattataatc tttaaaacta
    15001 tttcttccat tttttcctcc taaattgctt ctctactttt agattattta taccttacaa
    15061 acatataata tcttataaaa acaaaaaaac atgcacaact ttttccatca catggaaaat
    15121 tttgtgctta tccatagttc attatgaatt ttatataaga atatctttta ccttttcaat
    15181 gtcacattta cttagaatat ctagcatatt ttcatcctct gttaaattag atatttcttt
    15241 aaataattct ctaaatactt ttctatcatc atctttttta tatgctaata aaagtataat
    15301 ctttatatcc tttccatttc taatttttat tggtgttttt aaccttgcaa aacataaaac
    15361 agattgtttt atatatttca tgtctccatg agggaataat attccttttc caacattggt
    15421 tggatatgac ttttctcttg ttataataga ttgtaaatac tctttttctg cataatgttt
    15481 ttcaattaat aaattagtta aatatgttat agcttcataa atatcatcat gttctaactc
    15541 tcttattaaa taatcagaca tcattaatct gttcataagt ttatatgagg tgtctttttc
    15601 ttttattttg tttgtaaact ctttaagttt attctcatcc tcatcagtaa ataatggtgt
    15661 tgttataaaa attggttttt ggatattttc atactcttga aatgcaatta taaaatctat
    15721 agtcttatta aagttatctc tttttaaatc ttcctctctt aatgtttcaa taatttttat
    15781 gttatcaaat ctcttttcta tctttacttt taatagcata cttactccaa aactaaatgg
    15841 acatacaata cttgcctttt tatttgattc ccttttgctg tattttcttt ctagagaggt
    15901 ttgaaagtga agtgtaagat aaccaatttc atcttccgtt atttgttttc cttttaattc
    15961 aatttctatt gaacttgata ttactgtaaa taaaaaacaa aatttagttt ttatgttatc
    16021 taaaagtgga ttttctaagt aaacattgct tttcatttga tgcattgtaa cattcaaatg
    16081 acatattaat tgttcataaa gtaatatatc attattaaaa tttattcctg attcttctga
    16141 tactaaattt ataatttttt ttgtataact tgctaattct tcatttagat ataaagattt
    16201 cttaatatta ttactattta tttgtttgtt catacccaat attaaagatt ctataaacaa
    16261 tttttcttca taatttaata ctatggataa attagatttc aattcatctt ctatatccaa
    16321 tataagcatt tttagcttaa gctctttctt agtatttgat acttttaaag aatttcccag
    16381 ccttattcgt attatattta taagcaaaaa tagcatcaat tgcttgaatg atatatctgt
    16441 aagtcttata tccatatcct cctctatagt aagcattatg ttttttacaa ttattatatc
    16501 tactatatgg aagtactcta tttcatctat gtttaatctg tatttatcta tgtatttaaa
    16561 taagaatgat actaacatta ttcttatgtc tttctcatcc ccttcaatat atataccact
    16621 gcctttttta gtcgagacat ttaaattata attatttaat gctactgata atttacttat
    16681 ttcttctctg acaatatttt tatttatgta caacttatta gataaatcat ttaaagtaac
    16741 ttttttatta ttacaaagta gtaaatttaa tatctcaact tgcttatgaa gctcactatt
    16801 tactctttta acttcacctc gcaacttctg atttaccagt attttgtctg ttccatctcc
    16861 actaaattta attccaatat taggttttct tttgatataa gcattacaaa aagtagataa
    16921 ccattcatca atatatttgc aatcattcct tattgtcctt tctgaacact caaaatcatt
    16981 ggcaatctta ttaacattta taaactcttc ttttaatagc aatgttgtta ataaattttc
    17041 ttgtctttta ttcaaacata tcacctcttt atgactcaac ctaaatttat tatatataaa
    17101 aaagagctat acataaaggt atgctagctc tcttttaata tgatatttag ctgacctggt
    17161 ttttgacccc acactcgcct actggagggt tcgcttgggt tcgccgcact actactctct
    17221 ctcgatttta ttatcggtag gtcttacttc taagccgcac catgatcatt aacccaattt
    17281 ttagtgttaa cttacgtgga tccctttgcc tgcgactggc ttggactttc aaccacagac
    17341 ctaaatatca cttattcatt ttaatatttt aaactaaaat tgtcaatata taagattatt
    17401 ccataatata attatcattt gcttttgtaa gtagagtcaa tacaaaaacc ttattagcac
    17461 ctgataactt taaaagttta gagatttcgt ttgttgttag accagtagta aatatgtcat
    17521 ctattaaaag tatattttta ttatttatta atttaacatt ttctttaaca acaaatacat
    17581 tttttaattc ttcttttcgt tctttcttat tcaatttata tagcattctt gtatattttt
    17641 ttctacttat gcaatctaat aatggaatat ttactatttt acttaaatta aatgctattt
    17701 tttgtgcctg attaaaacct cttttattta gccttttttt atgaagtgga acaaataata
    17761 tataatcaaa ttttatgttt tctaaatata atttttcttt cattatttga gctatatact
    17821 tagccataaa ggttttttgg ttatacttca gccctaacac catctttttt gtcacatcat
    17881 tatattcaat acaagatatt gatttatcaa aataaaagtc tttattaaaa caataactac
    17941 actcttcaat aaattctttt tcaattgaat gacgtattat tggttttcca cacttcatgc
    18001 aaccatcttg tataaaattc atttctttaa aacaactttt acatattgaa taagtatttg
    18061 ttttctttat ggatttatca caaattatgc aacttatatt ctcaggatat ataaaatcca
    18121 aacatttatt aatcatattt ttaaaattct taataaaatt atcattgcat attttcatat
    18181 ttattctatc aacaaccctt cttgtttaaa tttatttagc ttatatccta aatttgaata
    18241 cctttgatta actctattat tttttatcat atattcaaga tatttcacat ctccaactaa
    18301 tacaactaat ttttttgctc ttgttactgc tgtatataat aaattacggc taagtaacat
    18361 aggaggagcc cacgctattg gcaatactac aactggaaat tcacttccct ggcttttatg
    18421 tattgttgta caaaaactat ggtcaatttc atctaattca tcatataggt atgatacaat
    18481 tttagtctgg tcaaataata tataaatagt tttcttatct ttatctatgt gataaacata
    18541 tcctatatct ccattataga taccttctcc actttctttt tggtcttctg tttcccattt
    18601 ttttgtataa ttatttttta cttgcattac cttatcgcca actctaaata atcttttaga
    18661 aaaactttct tcaactttaa atttttcttt tttgttaaga tacttctgta actctatatt
    18721 gagattagta actcccaatt caccttttct cattgaagat aaaacttgta tatcttttaa
    18781 tttatcaact ttataaaatt taggtaatct ttcattaacc aatcctatta tttcatttaa
    18841 aatttcttca ttagttgatt ttcttataaa aaagaaatct ttaccttttg tatttagata
    18901 aagtggttct ccattgttta tcttatgggc atttacaatt atcatacttt cttgcgcttg
    18961 tctaaatatt tcatttaatt taactacatt tataatatta gaattaatca tatccttaag
    19021 tacatttcca gcacctacag aaggtaattg gtcactatct ccaactaaaa ttactcttgt
    19081 tcctaacttt atagctctaa gtaaattgta cattaagata atgtctacca ttgatacctc
    19141 atcaacaatt attacatctg aatttattgg gtcttcttca tccttaaaaa aagttaaatc
    19201 atcatctgtt gaaaatccca tttctagcaa cctatgtata gtttttgctt ctttattaga
    19261 agtttcactc attctctttg ctgctcttcc cgttggagct gccaaagtca cacttttacc
    19321 attattttca aatatatcta tgatagcatt aatggtagtt gttttaccag ttcctggtcc
    19381 accagttata attaatactc cactattaac agattctttg actgctaaaa tttgtttttc
    19441 tgctaactta attttcttat cttcttctaa aacattaatc tcgttatcta tatctatttt
    19501 taagtcttca aattcatact gtgataattt tactatttga ctacatactc cattttcagc
    19561 taaatagtat ggcataaggt atataagatt ttcattgttt actttctcta tatgtatttt
    19621 ttgattataa actagcatca ttacacactc ttttattatt tctccattta gctccaataa
    19681 tttttccgaa tcttgtatta gaacatgctc tggtaaatat gtatgcccat tacttaaaga
    19741 tttatttaaa gtgtataaga ttccctgaca aattctatct ttagagtttt tatctatacc
    19801 aattttactt gctatactat cagctacttt aaatccaatt cctcttatat cttcagcaag
    19861 ttggtatggg tttttattta taacttctat tgcactgctt ttatattttt tatatatttt
    19921 taggcagtaa tttggtgtta ttccaaaagg agatagctgt attattatat ttctcaattc
    19981 tctgtcttct tcataacttt ttactatttg ttttactttt ttactcccta taccttctac
    20041 ttcttgtaat ttttctggtg aattttgtat cacctctaac gtatctacac caaacttatc
    20101 aattattctt tttgccattt tttcaccaat accatgtatc attccagaag ataagtaaac
    20161 ataaattcct tctaaggatg atggcgttac tggcataaaa ctattcactt caaattgagt
    20221 cccataaatc ttatgattta cccactttcc ttcaacttct atgctttctc caattgccaa
    20281 agtaggcata catcctacaa ccacaatttc atcattttca tttaccaggt gagctattgt
    20341 ataaccatta tcttcgtttt taaagactat ttcacttatc attccttcta atttttccat
    20401 ttttatctcc taaaaatact aataatcgct ccatttaaat gttagtataa ttttatatat
    20461 taaaactatg ataatcgtag aaaagtttta tatttttata cttaacgtaa tttccaacta
    20521 ttatacattt tatcagaaac aaaaaaaatc tgcatccata aaatatggta ccatgaaata
    20581 caggtaccat atttaaatag ctattttatt ttaattactc tatatttaga gtataaatta
    20641 aattccataa gtatattaca gtactaactt atttcagatt tattctcctt gtacccctca
    20701 gttttagtaa ttcttctata aagcatacat agtaaaatta aaattcccat gtaacctgta
    20761 tatggatata gtgtacctac taacttatca aatggcagta aacctcctat acaagctaat
    20821 atacttacca ctattgttat aattttaaat ttagggtggt catcttctac aaatcggtta
    20881 gttactgacc ataacaatgg tacagctgta gtgtatattc ctagtaaaag tactactgag
    20941 aataaaatac ctattattgg agatatctta tcagctaagt aaagtgctgg tatctctttt
    21001 gtgtatatat ttcctatgtt tgaaagtaat gccaaattca tcattatagc tgctgccata
    21061 agtgctactc cacctactat accaccccat acagcatctt tcttatttac tgcagaagct
    21121 cccattcctg tcaagaatgt tattactatt attacattgt aacaagtata aagtactccc
    21181 gagaaatatc catttgatgt agcagtttta atatttaaac tatcaaccat atttccagca
    21241 tttgataaag catcaatatt gctaaataaa ctaattgctc ctactaaaag tgtaaatatt
    21301 attattatag gtcctatatt acctagtatt ttagaaagcc ttgtcaaacc taatgaaaca
    21361 gttattaggg aaacgattgc cataccaatc ctacctacat atggatttaa tccatagtac
    21421 tctgatagtg tagctcctgc tcctgcaatc attattacta atgttccaaa taaaaacaat
    21481 ggaccaaacc attcaaaaaa tgttccaaga tattttccac aatatacttg ataaattttt
    21541 attggctcct taagtttcaa ttctcttccc ttatctataa cttctgctcc aaaccatgaa
    21601 aatagtacca tagagattat tccacctata atacccgcat atccataaaa cgtaaaaaat
    21661 tgcagtattt cttgaccagt tgcaaatcca gaacctatta cagtagcaac ataagctcca
    21721 gcaaaactta taaccgtttt aatatttact ttgcctgaca tacactactc ccccccaatt
    21781 aaaattattt ttaaagtgta agtttattaa gattatatta gactcattat tgtgatattt
    21841 caatatattt ttaattattt aacattagtt aacacattct taaccatcct ttaacatata
    21901 tcccataata tgtataaatt tagtagagta ttatataaaa tgtattgacc agattaaata
    21961 ctgtttattc attatatgat gataaattaa catttattaa aaatatttaa ctcataataa
    22021 aaaaactgtt agaaagtaat cgtaaaatta cattctaaca gctttttagt taaaaattat
    22081 tcacctaaag catcttttct taactgctca accttgtcag ttctttccca aggtaaatct
    22141 atatcagttc taccaaagtg accataagca gcaacctttt tataaagagg ctttcttaaa
    22201 tctaagtctc ttattatcgc tcccggtctt aaatcaaagt gtttatttac taattcaaca
    22261 agtttttctt ctgaaacctt gcctgtccca aaagtatcta taaatataga taatggtcta
    22321 gctataccta tagcatatgc aagttctatc tcacatttgt ctgcaagacc agccgcaaca
    22381 atattttttg caacatatct agctgcataa gcagcagatc tatcaacttt tgtagggtct
    22441 tttcctgaga aagctcctcc accatgtcta gaatatccac cataagtatc tattattatt
    22501 tttcttcctg taagacctgt atctccttgt ggtcctccta taacgaatct tcctgttgga
    22561 tttatataaa ttttagtctc ttcatcaagt aattctgctg gtataacttc cttaataaca
    22621 tgattaatta aatcttctct tattttatca tttgatactg tttcacaatg ttgagctgat
    22681 ataagaactg tatgtactct tacagcttta cttccttcat attcaacagt aacttgagtt
    22741 tttccatctg gtcttaaata atcaactaaa cctgtttttc taacttctgt aagccttctt
    22801 gataatttat gtgccaaaga tatcggaagt ggcattaatt ctggagtctc attacatgca
    22861 aatccaaaca ttataccttg gtctccagct ccaacttttt ctatttcttc ttctatctct
    22921 tctccagtct tactttctag accttcatca actcccatag ctatatctcc agattgttca
    22981 tcaatagaag ttataactgc gcaagtatca caatcaaatc catattttgc tcttgtatat
    23041 ccaatttctc ttactgtttc tcttactaat ttaggaatgt ctacataagc agatgtactt
    23101 atttctcctg ctactaaaac taaaccagta gtaactgtag tttcacaagc tactctagat
    23161 tgtgggtctt tttctaataa tgcatctaat attgaatctg atatttggtc acatatctta
    23221 tcaggatgtc cttctgtaac tgattctgac gtaaataaat gtcttgccat ttttattccc
    23281 ccttatttat tcttaatttt tattataggt tttaccttat ttttaatttt agaaatttac
    23341 tcaaaataaa aaaacctctc ctttataaga agaggttaat aatcgtaatt acatactaat
    23401 taactgacct catctttcaa gacttttgtc ttgtaggaat tagcaccttt tcccatttgg
    23461 gtggttgctg ggcttcacag ggcctatccc tcagcctctc ttgataaggt aatcaagatt
    23521 taagttttgt tttactttac tagcttacta tttttttaaa attttgtcaa tatttttagt
    23581 catatttttt ctattccttt ttttgtaaga caaataaaat tttctatcta tctgaaattt
    23641 aaagttaggt ctttttaatg ctttttattc atatattttt ttgaaggggg tgttcctatt
    23701 gtatttagct aaagtaaact acagcgatga tgatgttata cagatgctaa gttcagttct
    23761 aaaaactata attaatgaaa atacgattgt tgtttgcata ggaacagaca gggctatagg
    23821 tgatacttta ggccctttag ttggaacaat acttaagaac agtaacttta aataccccgt
    23881 ttacggtaca ctagataacc ctattcacgc cttaaatata tatgagtctt tagatactat
    23941 aaaaaataca catatacaag gtaacttctt ggctatagat gcttgcttgg ggtcgcagag
    24001 taacataggt aatatacaga ttagagaagg acctatcctg cccggaaaag gtgttggtaa
    24061 aaaactacct caaataggaa attattctat agtaggaatt gtagataaaa ttgatgaaaa
    24121 taataaactt tcatttaata atatacgcct tagctttata ttagatttag ctgaaacaat
    24181 agccttggct ttattagtat ccacctaatc taaatataaa ctaaatgata aaattcttta
    24241 tataaaaagg tagatatttt agagtatctg attaaatact ccaaattatc taccttttta
    24301 cttttatatt tcaagttttt atttttctac taaagcaaac attaattcac ctttacagac
    24361 aagttcttca cctacatatg ccttagcatc tgccttagct atattttttc taagagttgt
    24421 catttcaatt tccatagtta atgtatctcc tggtcttact tctctcttga ttctaacttt
    24481 atctatacct gcaaatacac ctagttttcc tttgttttcc tctacagaca tcattgccac
    24541 tccacatact tgtgctagag cttctactat tagtactcct ggcatcaaag gatattctgg
    24601 aaagtgacct tggaaaaatg gttcattaac tgttacattc tttataccaa cagctctttt
    24661 cccaacttct agctctgtaa tcttgtctac taataaaaat ggatatctat gtggtattaa
    24721 tttttttatt tcatctatat ttaacattct aaactctcct aattattttc ttttgaaaga
    24781 cccaccagtt tcaagtaagt ctattgaacc taaagctatt cctgtacctt ttgcaacaca
    24841 agataatggt tcatctgcta ttgtaacagt tattcctgtc ctctgttcta ttattttatc
    24901 taatccatat aataatgaac ctcctccagt cattattata ccgacatctc ctatatctgc
    24961 tgctaactca ggaggcgttt tttccaaaac agcatgagta gcaactacta tttgctcaac
    25021 acattctcta agtgcttcca acatttctgt tgaatttata gttatagaac taggaagtcc
    25081 tgtaattaaa tttcttcctg ttatcttcat atatttttct tcttctctct taaatgcaga
    25141 acctatttca aacttgattt tctctgctgt tctttctcct ataagaagat tgtgttgttt
    25201 tttcatatat ttaactatag catcatcaaa tctatctcca ccaaccttta tagattcact
    25261 tacaacagct cctcctagag atattacagc tatatctaca gtacctccac caatatctat
    25321 aatcatatta ccattaggct gtgatatgtc tacaccagct cctatggcag ctgctattgg
    25381 ttcttctatg atatatactt ctctagctcc agcttgtctc gtagcatctt ctactgctct
    25441 tttttccact tctgtgacac ctgttggtac acataccata attcttggca taaaaaatct
    25501 tccaaatccc tttttatcaa ctatcttatc tataaaagat tttaacatct tctcagttac
    25561 atcataatct gaaattacac catctcttaa aggtcttata gcgactatat tacctggtgt
    25621 tctccctatc atctttttag cttcttctcc cacagctaaa acagaatctg ttcttttgtc
    25681 tatcgccaca acagatggtt cttctaaaac gattccctta ccactaacat aaacaagcac
    25741 atttgcagta cctaaatcta tgcctatatc agctccagcc attttttcaa ctcctttatt
    25801 tataatatta gattatatta taagcctatt ttaatactcc tataataaag catatacaaa
    25861 aatctcgctt taatcaagag aaaataaatc ttttgagagt attattattt tgctatttat
    25921 aattacttaa tatatttctt tttataattt aattcaataa aaaaagtagg tttttaacct
    25981 acattttttt atgttccttc tcatatttta atttcgttgc taatccccct ctaatatgtc
    26041 tctctgattt atttttgtcc agcacatttt taacttcttt ggcaagggat ggatttattt
    26101 ccttcaatct ttctgtaaca tccttatgaa ttgtactttt acttactcca aatgtcttgg
    26161 cggtttgtct tactgtagtg tttttttcaa gtatatattt tgctaccact atggcccttt
    26221 cctctatatg agatctcaaa gattaccccc ctcacataat agctttaatg atatatatgt
    26281 aaaaacacca aaaaatataa caagcttaac caaatatgat taaacttgtt atacttttac
    26341 ttaattagac tcattgggtc tatagatttt tctccactat acgcctctaa atgaacatgt
    26401 ataccttctt cactttctat ttgagaagta ctacctacag tacctagaga ttgtccttct
    26461 gttaccttct gttctttctt aacactaaca tttttatcta gatttgaata tactactaca
    26521 atgttattgt cactttttat tttgactgat tgcccatatt cttcatcatt aaatacatct
    26581 actactattc cgtttaatag agattttact tctttgcctt tagtacaact aatatctaca
    26641 cctttatgag tttcccaaac atcaagagtt ttagaataac taggttcttt ttctgaatat
    26701 cctcttatta ctttatctcc aatgtaattt aattttgttg tactcgattt attttcctct
    26761 tttgacttag ctttttctaa gttttgtttt gaatccgttg cagtagggat agcatctttc
    26821 ttgtcttttt caattaaatg tatttcctct tcactatctt tattagcatt ttccataatt
    26881 cctttattag aagccaattt atctacatta ttatttgtaa accaaactcc acctactgct
    26941 aataaacaaa cacatacaaa caaagataaa taaaaaccat ctttttctaa cagctttttc
    27001 ttcattttgc acctccaaat atatttctac atttggatta ttgacgtgtt tttggcattt
    27061 tatacaatta ttagtatata tcttttattt ttgtatctgt gtagtaatgt gataatatat
    27121 cataatattt atagccctct tctgccattc cttctgctcc ccactggctc ataccaacac
    27181 catgaccata tccttttacc acgaaatcta tgtatccttc tccaaatttt atgtcaaagt
    27241 ttgcagagtt caatttaaaa acagttctaa tgtcttttcc agtcaattct ttatttccta
    27301 attttatttt ttctacagtt ccggcatcac ttcttttagt tattgaaact tgtttactta
    27361 aattattcac atctatcaca atagtgctat atgcccttct aagactttta acaaaatctg
    27421 tgttacttat tttaagtgtt gacgcaaatt taggggaata cttatcataa ggactttcca
    27481 cagactttaa ataaggatat tttgcagaaa atacttcttc actattttct gtttttcctg
    27541 aagatgtaga aaaataaaga ggaagtatcg ccttatcatt ataagttatt atttgacctt
    27601 tagttcctct aactgcttct tggattttgg aatatttatt cttaatccat tcttcaccat
    27661 ttaatttttt aagagtatca taactcttat attcttgaca atgtttataa tcagtacata
    27721 ctactgcatt tttatgttta ctagatttac catgttcctg tttatatact acatatgttc
    27781 tagctgctac agattgagct tttaaagctt ctatatcaaa ctctgaagac atctcaccag
    27841 ctagtactcc acataaatag ttctccatgt ccatcttttc ggttttccct gttatatgat
    27901 tatatacatt gataattggt gcttttttat ttacagtttc atagctcact gtactttttt
    27961 cttctttatt acctttgtct tcaatatcac tttttttaat agttttgttt atagatactg
    28021 ggctctctgg tttttcagtt aattctacat ttttataaga aactaaagtt ataagtgatg
    28081 gaactaacac agagcaagtc acaaatccta acaaaacaac caatgggttc ttcatatact
    28141 ctcctcctta gcttttaaaa ccttgtattt aagtatatga acaaccctat taatttataa
    28201 ctttcttcta taaaccaatt attaatcttc tactatttcg atttgaccac ctagtgctgt
    28261 tatcttctta tctatatcaa cataacctct ttggatatgg tatatttctc ctatttgagt
    28321 ttctccttct gctataagac cgcataaaat aagggctgcc cccgctctta aatcagtagc
    28381 gtttactgct gcaccgtgta gctcatctac accattaaca accgcacttc ttccatctat
    28441 ttttatatta gcacccattc tattaaattc agcaacatgc ataaatctat tttcaaaaac
    28501 agtttctata actacaccag ttccattggc tacagtaagc atagccataa attgtgcttg
    28561 aacatctgta ggaaatcctg gatgtggtaa agtttttata tctattggtt ttaatacttt
    28621 tggacctaca actcttacag aattgtccat ttctgttatt tcgcaaccag cttctcttaa
    28681 tttagctaca actggtttta aatgctccat taaaacattt tcaacagtta tatcaccttt
    28741 tgtcatagct gctgcaacca tataagttgc tgcttctatt ctgtctggaa ttacattatg
    28801 ttctgctcct tttaactctt taacaccttt tatttttata gtattagtac ctgcaccttt
    28861 aacatctgct cccatttcgt tcaagaagtt tgctaaatct actatttctg gctcttctgc
    28921 tgcattctct attattgtag tcccttctgc taaagaagct gccatcatta tgttttcagt
    28981 tgctccaaca gatgggaaat ctaaatataa tttatttcct actaactttt ctgttgctgc
    29041 ttcaacaaaa ccatggtcca tttctatctt tgcacctaag gctttaaatc cttttaaatg
    29101 taaatctata ggtcttgttc ctatagcaca tcctcctggc atagaaattt tcgtactatt
    29161 aaatctagca agcaatggtc ccataaccaa aaaagaagct ctcatctttc ttacaagctc
    29221 atatggagct tcacaagttt ttatattgct tgcatcaaca gtaagtgtat tttctttata
    29281 ttcaacttct gctcctacat gtcttaataa atctgatata acatgaacat ctcttaaatt
    29341 tggtactcca tttaatgttg attttccgtt tgctaataaa gtcgctgcta ttattggtaa
    29401 aactgcattt tttgcaccat ctattttaac actaccttta agtggattac tcttttttac
    29461 tattatctta gccatatttt ttgatctcct cataattaat aatctaattt tataattggt
    29521 gtggcaatat atatgagtgt ttttccgtca tcctcattat atttaattcc tatatttaaa
    29581 ttaatttttt cccctaaata ttctaattca taatcattta atactttact atatgctgtt
    29641 atagataaaa agtttacgtc tttaacttca tctatttgct tagcactcat attatataat
    29701 attttttgta aaatatcatc atatttgttg ttttgtaacc tttttgtgta ttctccaaca
    29761 atacaagcag acacatctac ttgagatgaa tatttattta acacattttc tacgttacta
    29821 taaataacct ctttatgttt atatactttg ttatccaata tgtcaactac tatgtaactt
    29881 tctttgttat ttttatttat tccttttata gaaatttctt tatcgctacc ttttatagta
    29941 gcacataatt gtattttatc gccagaccct ttttgattcc aatttattcc ttttttatcc
    30001 aactttagat tgttaattat ttctataaaa atattatgaa attctttatt tgataaatta
    30061 tgatttataa cagaattaat tttaacatta taaaatttaa aatttgcttc tgatatttca
    30121 agagctttac ttaatctatt gtatctagta tttatacttg tatctgcata agatattgtc
    30181 attcctataa tcattaatat aaaaaagctt atagctaatt ttactttttt catagtaaaa
    30241 aatccccctt aaatatgttt ttctgtataa tagaattatt aacaaattta agagagatat
    30301 actttctcaa tatgtatttt tatacatttt tgtatttttc atctaataat ctctttcaaa
    30361 ctcttcaatc tcaattttct ctatccttct cctggacttg ccaattcctt atatgtatca
    30421 aatacatctt gcattaattc ttatatatta tcttctgtaa tataaattac ttagtctata
    30481 cttaaatcta taatctataa tctataatct ataatctata atctataatc tataatctat
    30541 tcatatttta ccagcaaaat aaacagataa tactttttta tcaatttcca tagctccaaa
    30601 tgaacatacc taagtacact tcccaataca caatttacac actcttccta ataaatcaaa
    30661 aacacctttg ttttaaacaa ctctattgta ctttcaaaaa ctttatttgt gttctcccac
    30721 ttcaattaaa tttgaattat caagactcca gcccatccat ccatcactat aaagggaggt
    30781 attttccact cctatgacat ttgcatatgt taatacttca gcagcacgcc atccacttcc
    30841 acacataaat ataagctgtt tattcacatc tatattttcc ttgtcccaca tttctaaaat
    30901 ctcagattta tttctcattg tattatcaat attacgatat tcttctagtg aaacactatt
    30961 actacctgaa tgtccatata aagctcctgg tatacgccct tttttatcat aatatgtata
    31021 tccagatact tttcctatat gttcatccca agtacgatta tctactagta taaacttatt
    31081 tttttctttt agtttttgtc ttaattcaga tgtagtaaca actaattgtg agttaactgg
    31141 tatatctgct ccaaaattag tacatgaatg ttttggatta ctaataaatt ctaattcata
    31201 tcctgctgat aaccatgagt ttgttccacc attcaaaact cttacatctt ttactcctat
    31261 atatctcaat ataacagcta gtctataaga tgccataggt gtaatacttg aaactattac
    31321 agtatcatcc tttgttagtc catagtctaa agcaaactta gttaagttat catcattatc
    31381 aagcatccat gttggaggag gttctataat atcagtattt acatgaacac ttgtaggtat
    31441 atgccctttt gtatacgatt cttctcccca actcgcttca atcattttta tattctttga
    31501 atcttcaaat gtctcaggaa tttttccatc taatatatct tttattataa aagcaggaac
    31561 tatcatttga taatttttat atctttccat tggaagattt tcatcgtctg cccattgttt
    31621 aatatcatat ttatatagat atttatatcc ttttttagat aaataatctg ctacaaccaa
    31681 agcatctttt ccatttgcat catacaatac tatattttta tctaaatcaa ttcttttagt
    31741 tttcaatgct tgttctagca cttcatcttt tctatctgag tatacattta accaattagc
    31801 agaaaaatct actgctcttt ttatatgccc acctcttttt actccatcta acttccagcc
    31861 attataagca tcatttaagc gagtatcaac tataacccaa gcgttatctt gcattttttc
    31921 ttgtaaaact tcagtagata ttgccttaag attaacaaaa taatcttctt tcttttcttt
    31981 tttaaaatca tttgtcataa tttatactcc caatttaaaa aaatttttat ttgaaattat
    32041 tttactaaaa taaagtaatt ttcttatact ctattagctt cattatattc ttttataaat
    32101 agttccttat attcttattt taaactactc actttatctt atcacaaggt tactaatacc
    32161 ttgtatttta atttttctca taagctatta tttttttatg tctgatattg actatatgtt
    32221 tattaagtac tcactatatt ttatatgaac tatatttatc gttccctata cctggtatgg
    32281 tctatatatt tatcatttta cagatgattt aatatcaatc tttttataaa atttattata
    32341 gcaaaaacaa aacaaattta atatttatct aaaaaactca tcctataaaa tacaaataac
    32401 aggatgagtt tttttcaata aatattatct atttaatttt taatctgttt ttattcaact
    32461 gttactgatt tagctagatt tcttggctta tctacatcgc atcctcttag tacagagaca
    32521 tgatacgcaa gtaattgcat tggtacaact gctaatattg gtgataaaat atcatctgaa
    32581 tttggtatat agattatttt atcagcagct ttctcaacat ccttattatg gctttgagct
    32641 attgctacta cataagctcc tcttgctctt acttcttcca tattagaaac cattttttca
    32701 aataactttt cttgagtagc tatggcaatt acaggagttc ctttttctat taaagctatt
    32761 gttccatgct ttagctctcc tgctgcaaaa gcttctgcat gtatatatga aatctctttt
    32821 aatttcaatg aaccttccat tgctaagcta tagtctatac ctcttcctaa atagaatgca
    32881 tgttcagaac taacaactgt tttagcaact tcttttatat attcttcatt gtctaatatt
    32941 tcttgtattt ttgatggtat ttctttcatc ttagaaatca tactatcata aaattctcta
    33001 gttattgttc cttttttgat agcaaagtct aaagctatca tgtataaaga agttatttga
    33061 gtagtatatg ccttagttga tgcaactgct acttcaggtc ctgcccaagt atagaataca
    33121 tcatctgatt ctcttgctat agatgaacct acaacgttag ttattgaaag tattcttgct
    33181 cctttagcct ttgaatctct taatactgct agagtatctg ctgtctcacc agattggcta
    33241 actaatatta ctaatgaatt ttcatctaca aatgggtcgc tatatctaaa ttctgatgct
    33301 atatcagtta ttactggtat atttacaaat ttttctatgg catacttacc aagtaatcca
    33361 gcattatatg ctgttccaca agccactata taaactttat ttattttttc taagtcttct
    33421 ttagaaatat ttatactgtc tagaattata tttccattat catcaagtct tctttctaaa
    33481 gtttctctaa ctcctgttgg ttgctcatga atttcttttg acataaagta gtcatatcca
    33541 cctttagacg cagcttctac atcccaagtt acatgaaata cttctttttc aacaagattt
    33601 ctattagagt cataaactgt tacattttcg tcttttagat gaactacttc tccattttct
    33661 aagaaataaa catctctagt atattttaat aatgcaggta tatctgatgc tataaagttt
    33721 tcaccttcac caacaccaac tacaagagga ctatcttttc ttactgcaac taattcattt
    33781 ccatgttctt tacatataac acctaatgca taagctcctc ttaattttga tatagttttg
    33841 tatacagcat ctaataaatt accctcatag tatttatcta ctaaatgtgc tattacctct
    33901 gtatctgttt gagattcaaa cttaacacct tcagaaatta attcttcttt tatttccata
    33961 tagttttcta ttatcccatt atgaacaact gctattgttt tggcttgatt aaaatgagga
    34021 tgtgagttta catcagatgg ttctccatga gttgcccatc tcgtatgccc tatacctaaa
    34081 tttccatcaa taggattctt ttctaagtct tctgcaagta cagaaagtct acctttaaac
    34141 tttctaatat taagttcttc ttcattagaa ctattaactg ctactcctgc tgagtcataa
    34201 cctctgtact ctagcttaga aagcccttct actataactt ctgctgcctt tctacttcct
    34261 aaatatccaa ctattccaca cattatttgt gtcctcctaa aaatattatt tatatatttt
    34321 aagagttagg tcatataagt ttactttggc ttccctttgt ccataggatt gctcctatta
    34381 tttaaagaag tctttcgatg gtaaacacac cgcagagcat ccgccgacaa ttcgattaac
    34441 tctgacctcg tcaacttagt tcctatatta ctcaataatt atgtctataa ctaagttctg
    34501 gcgcttagaa aggtgtcctc agtatttgta tctgagaata ccctttaaat tacgataatt
    34561 tttcttgtat aagattagct aagtttgttg ctaattcttt tatttgacct tcctctttac
    34621 cctctaacat aactctaact aatggctcag ttcctgatgg tcttatcaaa actctaccat
    34681 ttccgtttaa aatagattct attctttcta tctcagtttt tatttctgga tattccatat
    34741 atttgttctt attttcattt tttattgttg catttactaa tacttgtggg tattggctca
    34801 ttattgatgc aatttctgat aaaggtttct tttcttgcaa gattatattt gcaagtatta
    34861 gagaactaag cactccatct ccagtagtgt tgtaatctaa aaatatcata tgtccagatt
    34921 gctctccacc aagattatat ccatttttaa ccatatcttc taaaacatat ctgtctccaa
    34981 ctgctgtagt agataaattt attccatttt ccttagcagc tattgtcaac cctatattac
    35041 tcataactgt aactaccaaa gtatcttgcg ccaatttatt atttttcttt aaatttaagg
    35101 cactcaatat cattatatgg tctccatcaa caatttgacc cttttcattt actgcaatta
    35161 atctatcagc atctccatca tatgcaagac ctaagtcagc attatgttcc aaaacagccc
    35221 tttgaagttg ttctggatgt gtagagccac atttatagtt tatattattt ccatctggtg
    35281 aactatttat tgaaattaca cttgctccta gttcatcaaa cactattggt gccactttat
    35341 atgacgcacc attagcacag tccaaaacta cttttaatcc cttaaagtca gtgcttatta
    35401 ttgattttag gtagtctaca tagtctcttt gtgcacaatg ttcatgtaat tttcttccta
    35461 cattttcacc aattggaaga caatcaattt tgtctatatc gtctatatat tcttctattt
    35521 tcaattcaat ttcatcatct aatttatatc cattcttatt aaagaatttg attccattat
    35581 attcaacagg attgtgagat gcagatataa ccacaccaca atcagcacca tattttctag
    35641 ttaaatatgc cactgcaggt gttggtatta ttccaacagt aataacatca catccaacag
    35701 acataagtcc tgctattaaa gatgcttcca acatatcacc tgatattcta gtatccttac
    35761 ctactactac ttttactctg tggtctcctt gagctaagac aaatccacca gctctaccaa
    35821 gtttatatgc taaatcacat gttagttctg tatttgctac tcctctaact ccatctgttc
    35881 caaaatactt tctcactaaa gattctcctt tcataattga aaagcttgta atttgtatta
    35941 tatttttata gttcataaaa tatcattata ggcttaattt cagtcataaa aacattttaa
    36001 tacttatcag caaaaaagac aatagaaata attctattgt cttttttttg acatcttttt
    36061 ctttattagt tagttgcttt agaacatgta gcatccatac ctttttgtaa agcttctctg
    36121 tttaatggaa cgaatttttc ttttctaggt ccgaaaactt ttttgaatgc ttctagtaca
    36181 gattctatat ctacagtatc agatactttt aagtaagcgc ctaacattat catattagca
    36241 actttatcat ttcctaattc agctgctaat tcatttgctg gtatataata aacatctaca
    36301 tctgttctat caactttttc ttttattaaa gaactattta ctattatttt tccaccagga
    36361 ataacatcat ctttgaattt ttcaaatgct ggtatattta ataatatagc tgcagttgca
    36421 tcatctgtta taagtggaga accaactggc tcattagata cagttactgc acagtttgct
    36481 gtacctccac gcatttctgg tccataagat ggtaaccaag aaacttcttt tccttctagc
    36541 attcctgcat aagtaagtaa ttgtcccata gacataacac cttggcctcc gaagcctgca
    36601 catattactc tagctgtaga catttacttc acctcctcaa cttcaacatt tttgaaattt
    36661 cctaatggat agtatggtat catgttgtct cttaaccatt gtaaagcatc gtttggagct
    36721 agtccccagt ttgtaggaca agtagataat acttctacta ttccaaatcc taatcctgct
    36781 tgttgaactt ggaatgcttt ctttatagct tttttagctt gtctaacatg agctggagta
    36841 tcaactgcta ctctttctac aaatacagct cctgttaatg tagctaacat ttcacttact
    36901 cttattggat aaccttgagt ttcagcattt cttcctgatt gagcagtagt agctctttgt
    36961 ccaactaatg tagttggagc catttgtcca ccagtcatac cataagttgt attgtttaca
    37021 aatatagtag ttattttttc tcctctagca gctgcatgaa ctatttcagc agtaccaatt
    37081 gaagctaagt ctccatcacc ttgataagtg aatactgtat tttcaggatg aactcttttt
    37141 attcctgtag cagcagctgg cgctctacca tgagcagctt cttgagtgtc acagttaaaa
    37201 tatttatatc ctaaaactga acatccaact ggaacaacac ctacagcatc acctaatact
    37261 cctagttctt ctaatacttc accaactaat ctatggatta taccatgtgt acatcctgga
    37321 cagtagtgag tttgagtatc ttgtaatcct tcagttttct taaatacaac agccattatc
    37381 tagcacctcc tgcaaccaat tctccggcaa taattttttt agctttttca actatttctg
    37441 ctggtgttgg agtcattcct cctggtcttc catggaagta aactggtaat ttaccttcta
    37501 ctgccatttt aacatcttcc accatttgtc ccatactcat ttcaacagtc aatatgtttc
    37561 tagcattagg aatttggttg aatgcttcga atgggaatgg ccataatact ttaggtctta
    37621 taagacctgc ttttattcct tcttctctta atatatcaat tgcgcttttt actactcttg
    37681 aagtagttcc ataagctgca aatacaaatt cagcatcttc tgttttgtac atttcgtatt
    37741 gtacttcatt cttttccatt gcatcaaatt tttcttgtaa atgccaacaa tggtcttcta
    37801 gtacttctgg ttctaagtat agagagttta ttacattagg ttttcttttt cctttagttc
    37861 caacagttgc ccaatcttta ggaggcaatt ctcttttctt ctcaggagct ctaaattcaa
    37921 ctggctccat catttgacct atcataccat ctgcaactac cataactggt gatctgtaat
    37981 aatctgcaac gtcaaaagct tccattatca tatcaacagc ttcttgaact gtagctggtg
    38041 cgaatactgg agttctgtag tctccatttc ctccacctct tgtagacata aagtagtcag
    38101 cttgtgaagg ttgtatactt ccaagtcctg gaccacctct cattacatta agtacaacac
    38161 aaggaacttc tgctcctact gcatatgtta taccttcttg ctttaaagca actcctggtg
    38221 aagatgaaga agtcataact cttgctcctg cacctgctcc accataaacc atgtttatcg
    38281 cagaaacttc tgattcagct tgaacgaacg ctccacctat tttaggtaac tctcttgata
    38341 aatattctgg taattcactt tgtggagtta ttgggtaacc gaagaaatat ttgcaaccag
    38401 cttctatagc tgcttttcca aatgcttcgt tacctttcat aagtatctta gccattaaaa
    38461 ttccccctta aatattaatt aaattaatct ctttctactg ttataactac atctggacac
    38521 atagttgcac aacttgcaca tcctatgcat ttttccattt caaaaacagt tgctgggtta
    38581 tacccttttt tgtttattac atttgagtct aattgtatga tttttactgg acatgcttca
    38641 acacacaatc cacaaccttt acaacgttct tgattaaaag atacttttcc tttagccatt
    38701 tacctaagcc tccttaaact ttctatatat ttgagctcaa actacatcca gtcttctctc
    38761 atatataatt ttataggaaa aatttctcct tctaaatctt ttggtaactg ttcaacaaga
    38821 ttttctaaac atgcaacata tcttatcggt atattacatt tttcagaaac ttcttttgca
    38881 actttctgac ctcttaaaac atcatcaata gtagtaaacc taataagatg agtattatta
    38941 attagacctg ttacctttaa tttagaagat ttttctattt cttttatata ttggataact
    39001 tcttcagaag tttgagtttt ctccctattt gcatttatta caaaaagcat atcataatcg
    39061 ccttctttta acaaatgact aaatcttcct ataaccctag cacctacatt atctccacct
    39121 aaatctataa cagaattgta tgaatggtct accataggag acattacctc tgctgatact
    39181 gctggtaaat ctaatgttgg agcattaatt gagctatcta ttggttgtat acctaaggat
    39241 ttcattaact ctttcttttc tctagttcta aagtatacat ttaccacatc taaatcggct
    39301 atagcaactt tgccagaaac catatctctt aacttaacaa catagttcat cgcaaattca
    39361 cttttaccac ttccatagtg accagttatg attcttattc ttttatcatt agttatcata
    39421 aacttgagcc tcttcttcac cagttaaaac tctaagtcca ccttgagcta atgcaatcat
    39481 ttcatcttca cctggataaa cttttacatc tgctataaat ttaactctat ctgcaatcat
    39541 ttctgtaaac atttttgaat atgcgattcc accagttaat aatattgctt ttacatctcc
    39601 cttaagaact gcagcactag ctcctatttc tttagagatt tgatatgcca tagcttcata
    39661 tactaattta gctttttcat cgccagcttc aattctttct tcaacttctc tagcatcatt
    39721 agtgtttaag tatgcaacta atccgccatt accttttatt ttctttttaa tttcatcttg
    39781 agtatatttt ccactaaagc acatttttac taatgcacct actggtagtc caccacttct
    39841 ttctggagag aaaggtcctt ctccatctaa tgcgtttgca acatctacta ttttaccatt
    39901 tttatgagct ccaacagaaa ctcctccacc catgtgtgca actataagat ttatatcttc
    39961 atatttcttg tttatttctc tagcatatct tcttgctatt gccttttgat ttaaagcatg
    40021 tactacactt gctctactta tttcaggcat accagaaatt ctagcaacat cttctaattc
    40081 atctacaaca acagggtcta ctatgtatga aggaacattt acttcttcac ctatttgttt
    40141 tgctattatt ccacctagat ttgaagcgtg ttctcctaaa actcccactt ttaaatcttc
    40201 aatcatagca gcacttactg aataagtacc accttttata ggtttaagaa gtcctcctct
    40261 acctactaca gcatctaatt cagatgtttt tactccacct tcttttagag cttcttctat
    40321 tacttgttta cgaaattcaa attggtcaga caccttctca tattttccta tttcttctga
    40381 agaatgtctt aaagtttttt caaatactaa atcctcatta tcaaatacag ctattttagt
    40441 tgatgtcgaa ccaggattta ttgttaagat tttaaatatt ttgctcattt gcctatttcc
    40501 cccttaaaat tatgcctttg ctgccattaa aacacctaaa gctattgagt ttagtttagt
    40561 ttcttcactg tctgctctag aagttaatat tattggtgct ttagccccaa ctataactcc
    40621 tgcatttttt gattttgaga agaatactaa agctttatat aatatgttac caccttcaat
    40681 atctggggct aataatatat cagctcttcc tgctacagga tgatttatac ctttatgttt
    40741 agctgcttct aaagatactg cattatcaat tgcaaaaggt ccaccaacca tacaaccttt
    40801 gatttctcct ctttcataca tttcttctag ttctttagct tcaactgtat ctttcatttt
    40861 tggatttact ttttcttttg cgcatattgc agcaactttt ggttcactta tatctaatga
    40921 atgtgctact gtgcaagcat tttctatgat ttgcttttta gtatttgtat caggagctaa
    40981 gttcatagct gcgtcagtta cgaaaaataa tctatcatat ccctctacat caaatactgc
    41041 tacgtgactt aatacatttc cagttctaag acctacttct ttatttaaaa ctgcttttag
    41101 tattattgat gtgtctacta agcctttcat taccatgtcg gcttttcctt gtgaaactaa
    41161 ttcaacagat tttagagatg cttctgctaa atcttttata tctatcagtt cataattttc
    41221 gatatccatg tctatgcttt ttgcaatttc tttagtcttt tctatatctc ctactaaaat
    41281 ggcatttgct attttttctt ttctagccat ttcaactgcc attaaaactt ctttatcttg
    41341 gcaacatgct actgatatag ttttaggtcc tctttctttt gcaaacttaa ttacttcttc
    41401 aaaacttctc atgcatttcc acctcatctt gaattttttg aaatagaact ttttatatct
    41461 caattaaatt ataactcgtt cagcaacgtt ttcctagaga taattgaaat atttaattca
    41521 aaatatttat ttaaatctat ttatcagtct tttttcatat atctagcgct ttttagattt
    41581 aacaaatatc tcattgtcct tattttataa tataactaaa ataaacaaga tgcaacaaaa
    41641 acataaaatt aacaaactta attttatagg tttttttgtc gcatcttgta acaactaaat
    41701 attcgaaata tttgttcatt ccacttcaac aatattaggt tctatattta tttttttaaa
    41761 ttgatatgtt gtttcatatt ttattttata tttactatct tcctcagggc cttgtgataa
    41821 atcaatatat aatgaaatat cttttttatt taacttactc aaatcctccg ttgactctac
    41881 aacaactttt atatctacat cagaaattac atttggatta tctatatttg ttgggatatt
    41941 tcttaattca atatctcccg cattatataa aaattctgca ctcaattgtt ttgtatcatt
    42001 taatttaatt gttatagagc tatcctcaag agttataccc tctggaattt ctaagctaac
    42061 ttctttttct tcaccattag ccaaatcagc taagtcaata ggttttgtct taatataact
    42121 tattttgtct atatcttctt tctttccttt tattgtaact tgttccttac ttaatttata
    42181 gtttttcagt acatcattat ctttattatc atttagactt actcttattg ggacaatttt
    42241 ttccttcaat agttctatct ttgcagtcac aaaggatgaa cttaatgtta ctccttcaac
    42301 cttagttcca tcactattta atggtattaa ttgcaatttt ttagaaaaat catctgtctc
    42361 attaccaaca tctagattag ctcttacact tttgacttct ttaactagag ttcttggacc
    42421 tgatacatcg acaaattcct tactaagttc aattttattt atatttcttt ttgaatttcc
    42481 acttacttct acctttatat ctcttttttc ttttatgttt ttttcaagat ttactactaa
    42541 aacatcatgc ttaaattcta gagtaacacc ttgaggaata tttgctttta aatatacttc
    42601 cttctgtcct tgtattgggt cttcaatggt tccatataca tgtatatctt ctttcttaac
    42661 actttgtacc tttgataaac taccttttat atatacatct gctgttaatt tttcgtctgg
    42721 atatataaca agatttttat cctttaaatc actcatatta cttatagata caggtatatc
    42781 ttcaaaccct ttagtttctt cagggtcaac tacagccata acatacatcc ataatgcaat
    42841 tgcacttaaa aatgatatta tttttatttt ggtattattt tttagtctat ttatcattta
    42901 aatataccac ctttgaaaaa acttcttgtt tctgtatttg tttttaaatt actacgtaat
    42961 atattcatca ttctttctct agaaatatct ctatataact tacctgcttt agcaattgat
    43021 acgccacctg tttcctctga tacaatcaga gttatacagt cagacacctc acttacccct
    43081 attcctgctc tatgtctagt acccaaatct ttacttaaat ccttactttc cgtcaatggc
    43141 aaaaaacatg cagctgcttt taccttagaa tctctaatta caacagctcc atcgtgtaat
    43201 ggtgtatttg gtataaatat atttattaat agttgcctag aaatttcagc atcaataata
    43261 gttcctgtat ttattatatc acttattcta gtttctctct ccattatgat aagagctcct
    43321 attttttgtc tagaaagtga atataaagct tctacaattt cttctatatt tctattgaga
    43381 gtttcttcag aagtatttac attctttcca aataaattaa actttgctct tcctatatgt
    43441 tcaagtcctg ctctaaattc tggttgaaaa attataagag cagctataac tccataatct
    43501 aaagctttaa gagatatcca ataaacggta tgcaatttaa aggtgttact aagttgagtt
    43561 gctaataaaa gaaatattat acctttaaac acttgttctg ctcttgtatc ttttataaac
    43621 ataaatattt tgtaaaaaat atacgctact atagatatgt caattaaatc atatatactc
    43681 attcttaata ttatattaaa aaatccattt aaaaaagaat ttatatctaa cacagtttta
    43741 tcctcctgtt tgataacttc ttaattattt acactaagta ttatactata taaatctaac
    43801 tctatacaaa ctatttaact tttatgtaaa taattctagt tttatatttg gagattgtta
    43861 aataatctct aaacacatta aaaatatcaa cttaataaat acatatttct tattaaagtt
    43921 agtttttact caaataactc aaaccatcaa ggcgcgaata caaaatagta ctcgcgcctt
    43981 aatgcagttt tatgttctaa aatatcttaa tatatctttg tttaatatat attaattatt
    44041 attatttata ttgttattgt ctatcttgtt attatttata tcattattta cattattatc
    44101 tatattataa ttttttattt tataaattgt ctatcttgta aattttataa aatatacctt
    44161 catctacacc ttacaatatc cagaacttac aaaatctaaa tcttatgtca taaagtctaa
    44221 actttacaaa ataaacctaa actgcattat ttttaaattt atattgtagt ttatatatta
    44281 ttctctactt atcagaatcg actattttga aataagtgaa tgaccagtca tttcttctgg
    44341 cttttctaac ttcatcatat ctaaaacagt cggtgctata tcagataatc ttcctccatc
    44401 caataatttt gcactttcat attcttgacc aactacaata aatggtactg gattaataga
    44461 gtgagccgtt acagtttttc ctgtttccgg gtctaacata tattcagcat ttccgtggtc
    44521 agcagttatt attgcacttc cacctaactc tactatttta tctatcaatt ttcctacaca
    44581 agtatctaca gtttcaacag cctttatagc cgcttctata cttcctgtgt gacctaccat
    44641 atcaggattt gcaaagttta atactataaa gtcaaactta tcttctccta atttatctaa
    44701 agctttatct gtcaattcat atgctgacat ttctggtttt aaatcataag ttgcaacctt
    44761 tggagaaggt atcaatagtc tttcttcacc tttatttggc tcttctactc caccattgaa
    44821 gaagaaagtt acatgtgcat acttttcagt ttcagcagct ctaagttgag tttttccatt
    44881 tttagctaga tactcaccta aagtgttggc taaagattga ggtccaaatg ctatatgcac
    44941 attctctata gttatatcgt actctgttaa gcatacaaag aagtttttaa tatcttctct
    45001 tttaaatcca tcaaactctt cacaaactaa agctctagtt atttgtctag ctctatctgg
    45061 tctaaagttg aagaatataa ttgaatcatt ttcctttata cttccaactg gtttatcatc
    45121 tttcataata actgttggca ttataaactc atcatttttt ccatcatcat aagagttttg
    45181 aatagcttcc tcaatagaat ttgctttttc accaatacct ctaaccatgg cattgtatgc
    45241 aagttctact ctttcccatc tcttatctct atccatagcg tagtatctac cagaaactgt
    45301 agcaaattca cctactccaa tttcatccat actagcttgt acttctttgg catattctaa
    45361 tgcactttga gggtcagtat ctctaccatc agtaaatgcg tgaacataca ccttttgtac
    45421 gcctttatct tttgccattt tgattatagc cttaagatgg tctatatgtg aatgtactcc
    45481 tccatcagac aaaagtccca ttacatgaag agaattttct tttgcattat ccattgcttc
    45541 acataaaact ttattagcaa aaaagtcacc atcttttata gattttgtta ttctagttaa
    45601 atcttgatat actattctac ctgcacctat gttagtgtgt ccaacttctg aatttcccat
    45661 ttgaccatca ggtagaccaa catcaagacc actagcattt atcaatgtgt ttggatactc
    45721 tttttttatt ctatctaaat taggagtttt ggactcagca attgcatttc ccttaacatc
    45781 cttattgtat ccaaacccgt ccataataat taaagcaact ggttttttca tcatttcact
    45841 ccttagaaat taacaaggtc taggtaatca ttactagcta aactagctcc acctactaaa
    45901 gctccatcta tatcactttg acccattatt tcggctacat ttgaaggttt aacacttcca
    45961 ccgtattgta ttctaacttc attagctaat tctccatata atccttttat aacttctctt
    46021 atataagata taacgtcatt agcatcttca gcagttgcag ttttaccagt tccaatagcc
    46081 cagattggct catatgcaac aactactttt gataaatcat ctttaagaac attttctaaa
    46141 gctttttcaa cttgaacttt acaaacatct ttagtttttc cagcttctct ttgttctaaa
    46201 gtttctccaa cacataatat tgggtctatt cctacttcta atgcttttaa aactttcttg
    46261 tttacagttt catcagtttc attgaagtat tgtcttcttt ctgaatgtcc tattacaaca
    46321 tagtccatat ctatttcttt taacataaga ggtgaaactt ctcctgtaaa tgctcctttt
    46381 tcttcaaagt gcatattttg agcacctatc tttatatttg tacccttagt agcttctttt
    46441 aaatccttta ataaagtaaa tggtgcacat ataacagctt ctactttatc agaatttact
    46501 ttatctttaa cttcatttac aaattctaaa gcttctttta ttgttttatg cattttccag
    46561 tttcctgcaa ttataggttt tctcatttta caatctcctc tatatattag tttatttatt
    46621 atctaatgct gcaatacctg gtaattcttt accttctaaa aattctagtg atgctcctcc
    46681 acctgttgat acgtgagtca tcttgtcacc aaatcctaat tgattaactg ctgctgcact
    46741 atctccaccg ccaattacag tagttgcatc agtcaactct gccatagctc tagctactga
    46801 taatgtacca tttgcaaagt tttcaaattc aaatactccc attggcccat tccatactac
    46861 agtttttgat tcttttatag tttttacaaa gtttgctata gtcttaggtc ccatatctag
    46921 tcccatgtaa tcttctttta cattagcatc ttctgttact attggagtag catctggtgc
    46981 aaagtggtct gccataacaa cgtctactgg taataataag ttaacaccct tattattagc
    47041 tttttccatc atttctttag catattcaac tttatctatt tcaagtaaag aagttcctac
    47101 ttcataaccc tgagctttta agaatgtata agccattcct ccacctatta ttagattatc
    47161 aactttttct aataattcgt ttataacagc taatttatca gaaactttag ctcctccaag
    47221 tatagctgta aatggtctta cagggtttgc aacagcttct cctaagaatt ttaattcttt
    47281 ttgaattaag tatccacaaa ctctctcttg taaaaactcg cctgcaccaa ctgttgagca
    47341 atgtgctcta tgagcagttc caaatgcatc atttacaaat atttcagcaa gtgatgctag
    47401 ttctttagag aaattctctt cgtttttagt ttcttctttt ctatatctag tattttctaa
    47461 taatactaca tctccgtttt ccattttttc agttgctttc tttgcatttt ctcctacaac
    47521 attatcgtct gctgcaaata caacttcttt tcctaacatc tcagataatc tctttgctac
    47581 tggtgctaaa gataattctg gtttagcttc tccttttggc tttcctaagt gagagcataa
    47641 tataactttt gcccctttag aaattaaata ttgaatagtt ggtaaagctc cgttcaatct
    47701 attttcatca gtaattacac catcttgtaa tggcacatta aagtcacatc ttactaatac
    47761 tttttttccg cacacatcaa tatcttctat agtttttttg ttaagcattg acataaatat
    47821 ctctcctttt aataaagtaa ttttttattg ccaagtataa tatagtacat acttttaccc
    47881 tattaaatat atcatattat catattttta tatatagtat gtaacattat ttaggtgtat
    47941 tttatatatt ttttcttaaa aaaagtcctg agtccgtagt attaaactac aaactcggac
    48001 tttgtcaatc tacttttcta ttttctcaaa actgactatt ttgctaattg tgcaaagtat
    48061 cctaaagttc ttattaattg agaagtataa gacatttcat tatcatacca agaaacaact
    48121 ttaactaatt gttttccatc tacttccata acttttgttt gagtagcgtc aaataatgaa
    48181 ccgtagctta ttccgattat gtcagaagat actaacattt cttcagtata tccgaaagat
    48241 tcgtttgaag cttctttcat agctgcattt atttcttcaa ctgtaacatt ttttcctaat
    48301 gtacaaacta attcagttat tgaaccagtt acaactggta ctctttgtgc tgccccatct
    48361 aattttcctt ttaatgaagg tattactaag cctattgctt ttgcagctcc tgtagtgttt
    48421 ggaactatgt ttccagcagc agctctagct cttcttaaat caccttttgg atgaggacca
    48481 tctaaagtat tttggtcatt agtgtatgca tgtatagtag tcattaatcc tttttctata
    48541 ccgtatttat catttaatac tttagccatt ggagctaaac agttagtagt acaagaagct
    48601 cctgatataa ctgtttcaga accatctaat atgtcactgt ttgtgttgaa tactattgtt
    48661 tttaaatctc cagtagctgg tgcagatata acaactttct tagcccctgc ttgtatatga
    48721 gcttcagctt tatctttaga tgtaaagaat cctgtacatt ctaatactat atctactcct
    48781 aattcagccc atggtaggtc agcaggattt ctttctgctg ttacttttat ttcttttccg
    48841 tttactacaa aagcaccttc tttaacttct atttctccgt taaatcttcc ttgagctgta
    48901 tcatatttaa ataaatgagc taacatttta gcgtctgtta agtcgtttat tgcaacaact
    48961 tcgaacttat cttgttgctc catcattttt cttaatgcta atcttcctat tcttccaaat
    49021 ccatttatag ctactttaac cattttcgct acctcctata aagtaaatgt atgttttcta
    49081 gctctgtgct aggtcttgac ttttataata atgacaatat cttataagca ctttcttcat
    49141 ctgttacaag aaccatgttt ttatgcatat ttgatatagc taataaggct tctacttttt
    49201 cagtccctgc aaagattgca atagtttctt ttacttgttt aactgtttct aaatcaattc
    49261 caacagtgtt cagtttgtaa actatttctc catctttgtt gaaataatat ccaaatgctt
    49321 ctccaactgc atttttagat aatatctcat caaccttgtc ttctggaagt tttcttctct
    49381 ttgccatgac atctgctcta cctatactaa aaactaaaat gtctgttctt ttaatatact
    49441 ccaaagttgt cttaatctct ggcacttggc taagagtttt cattgcactt tcatccaact
    49501 catctggtat atgtaaaagt ttgcaattag aatgtaattt cttacttatt gaagcaacta
    49561 tattgttaga ttgcatctct acatcagttc ccatacttcc tctagctgga actattgtag
    49621 agtttggata tctcttatca gttttaataa cgtttgaaaa ctccaacata gtgcttcctc
    49681 ctgttattga aacgatatct ccatctttta aaatgcctaa gaaatactcg catccatatc
    49741 tagttacatc ttttagtaaa cttccatttt catcacagct tccaggaact agtattaccc
    49801 tttttattcc aagcttttct ctgactctat cttgtaactt agacaatccc atgatgtcac
    49861 tcatgatgtc cttaagttta tctaacaaat cagaaccttc tttagtgata gtcatcccag
    49921 atacagctac gtctataagt ccttgttctt ttaaaaactc agtttctgac ctgattattc
    49981 tttcactaat ctctaataca tttgataatg ttctcctacc tattggttca cttaaagata
    50041 tttgtcttaa aatagagtat cttctttcca taagttcaat tgcctgtgga atcaatttct
    50101 tttgaatttc taataagttc ttcataacat actccttatt gctttccttt cgggactttt
    50161 tttgtcccta gtgtttattt aagtcccaca agatattttt attttatagg atttgcgtat
    50221 agatttcaat agtttttcaa aaactttttt atttttttat tttataaaat atttctccac
    50281 cattttcaaa tcacagtatt ttttcttttt atataaggct ttttata
//
LOCUS       DAMJWP010000009        44990 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.009, whole genome shotgun sequence.
ACCESSION   DAMJWP010000009 DAMJWP010000000
VERSION     DAMJWP010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 44990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44990
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.009"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(155..934)
                     /gene="hisJ"
                     /locus_tag="PC700_000465"
     CDS             complement(155..934)
                     /gene="hisJ"
                     /locus_tag="PC700_000465"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ"
                     /protein_id="HDF2579791.1"
                     /translation="MKDGHIHSPYCPHGSKDDFEKYVQRAMDVGITEMTFTEHFPYAN
                     GFRDPAPENDSCMSIEDLPKYFNDVKKLKEKYEGKIKINVGSEVDFIEGYEEGIKENL
                     NKYGKELEDSLLSVHIIKIDDDYYCVDYSVEEFQNLIDKLGSIEAVYNKYYETLIKAV
                     NSDLGIHKPKRIGHLNLVRKFNQVFPYDYKGNTILEDLIKLIKEKGYELDYNVSGNRK
                     EYCKEPYIDGYLLELVKKYDIPLVLGSDSHCAEEINKYDII"
     gene            complement(1474..1824)
                     /locus_tag="PC700_000466"
     CDS             complement(1474..1824)
                     /locus_tag="PC700_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter"
                     /protein_id="HDF2579792.1"
                     /translation="MIFDKEIVRYHHGHHDHDHCHEHTHGDICHEHPHDHAHDHDHEH
                     SHEESSESKDEKTLKILLVHWINHNETHEEGFREWVEKARAIGKEETAKSIEKAIEYM
                     EEANKMLLEAKKHM"
     gene            complement(1840..2256)
                     /locus_tag="PC700_000467"
     CDS             complement(1840..2256)
                     /locus_tag="PC700_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HDF2579793.1"
                     /translation="MIIAVIDGMGGGIGAQIVSSLREELPTYVEIYALGTNSIATSSM
                     MKAHANKGATGENAIVVSAKKANIIVAPISVIIPNSMMGEVTCNISEAIADSEALKIL
                     LPIMPENVELVGLEGKPLALLVKDSVNLIKKEFNIK"
     gene            complement(2275..2466)
                     /locus_tag="PC700_000468"
     CDS             complement(2275..2466)
                     /locus_tag="PC700_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HDF2579794.1"
                     /translation="MCESSAFICKSNNELEKVMENVVNIDPCDGKIYLTDLLGEQKII
                     DGIIKEIRLMDHKIIIQEN"
     gene            complement(2617..3432)
                     /locus_tag="PC700_000469"
     CDS             complement(2617..3432)
                     /locus_tag="PC700_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HDF2579795.1"
                     /translation="MNQWRVIHNKSYEGAMNMAIDEAIFTAYKKGHNKPTLRFYTWEP
                     ACLSIGYFQKLEDEIDLDKCRCMNIDYTRRITGGRAVLHDNELTYSIIIGEDNPLIDK
                     SINLSYRYISEGLVKGLNLSGIETDNLNRGERISRENLSAACFNAHASYEVTINNKKV
                     IGSAQSRKDGVLLQHGSIILDFDVEKLFKLIKTKTPELKERAMKFTAKKASGIENEIG
                     RKIDIDILQKNIVKGLAEQFNVEFVEGDLTDYEKQLVKELYEKYKNEEYNKKR"
     gene            complement(3479..4417)
                     /locus_tag="PC700_000470"
     CDS             complement(3479..4417)
                     /locus_tag="PC700_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2579796.1"
                     /translation="MIRLSVGTAIELGILNKKSDIPPTTAYIMIGEKCINKCSFCSQS
                     IESSTRKDKLSRVIWPEFSKEEILDALKAYKGKNIKRICIQSMASEEAHNSVLDFINY
                     ISGKIDMPISLSAKLENDEQIKKFFSVGVDKIGIAIDASNKELYEKIKGNNYDEKLKF
                     ITEMSKSYPNKISTHIIVGMGESHEDIYNLYIYLKENDVTISLFAFTPVRGTKMEKIN
                     QPSIESYRRVQLMSYMINKGYPKEYFKFKNGYLNSIKLDNDILKDINKGYPFEIRGCK
                     DCNRPYYNERPGSTIYNYSRPLNQSEIDLAIREINL"
     gene            complement(4414..5286)
                     /locus_tag="PC700_000471"
     CDS             complement(4414..5286)
                     /locus_tag="PC700_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2579797.1"
                     /translation="MNKNLSEKMKIAFDIKLKNFGNKIEFAYPNQTLALSTTSNQCSL
                     KCAHCNGHYLNNMVPIEEYEEKVQSRNITSFLLSGGCSYEGDVPINTHINTIKNLKEK
                     GYRLNAHLGLMDKDSIVELCKYLDIVSFDLVFDDETIREVYKMKKSKEDYIEVYNTIQ
                     EHTEVAPHICIGLKGGQIKGEYEIIEYLQKNPPNKLTFIVLIPTKGTEYENVEPPELE
                     GVADILCEARINLPDTEINLGCMRPRGVYRKELDQLSIMCGVNRIVLPSRSAKNKAIE
                     MNMTINECKECCVL"
     gene            complement(5311..7239)
                     /locus_tag="PC700_000472"
     CDS             complement(5311..7239)
                     /locus_tag="PC700_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4445 domain-containing protein"
                     /protein_id="HDF2579798.1"
                     /translation="MIKVSFTPNNKEVYCNEGDILLEVARNADIFIDAPCNGNVSCGK
                     CKVKLLNGKVDTEKTRHITDDEWEQGYILACCTKVISDIEIEVPSKVSSSMHGMKIEG
                     SNKKEDREIFERAKKIIEEHNLQFKTNIKKKYIEMEEPNLDDNISDVDRLERYVRNSL
                     GYNEIDFRLDILRKMPTVFRKSDFKVTITYVQKQKKLTIINIEQGNKENSLYGVAIDI
                     GTTSVVVCLVDLYSKEVVDKASSGNAQIKYGADVINRIIYSTKKNGLETLHKAIVEET
                     INPLLKTIYERNGIDKEDVVTLVAAGNTTMTSLFLGVYTDFLRQEPYIPPFLKSPKLM
                     GENVGLFVNDSAYVYLAPSVASYVGGDITAGVLSAGIWSSEENVLFIDLGTNGEIVFG
                     NKDYMMSCACSAGPAFEGGGISCGMRASAGAIEKVIIDKETLEPTLKIIDECAPVGIC
                     GSGIIDLICQMITKGVIDRRGKIYRDLDNKRVRFNEHEIGEYVLAFKEEFDLENDIVV
                     NEVDIDNFIRAKGAIYSGAYTLVDSLGMDFSILDRVYIAGGIGNNLDIENSIIIGLLP
                     DIDREKFTYIGNSSLVGSYLALISKDAKNKLEEIGNQITYVELSVYPSYMDEFISACF
                     LPHTNIEQFPTAKKLLEE"
     gene            complement(7351..7728)
                     /gene="gcvH"
                     /locus_tag="PC700_000473"
     CDS             complement(7351..7728)
                     /gene="gcvH"
                     /locus_tag="PC700_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HDF2579799.1"
                     /translation="MKLLPELKYSKDHEWVKVIDGDVVYIGITDYAQDQLGEILFVET
                     PEVEDTVTKGVDFGVVESSKVASDLISPVNGEVLEVNEKLEDEPECINEDPYENWILK
                     VKLADVAELDTLLSDKEYEAGLE"
     gene            complement(7815..9941)
                     /gene="cdhC"
                     /locus_tag="PC700_000474"
     CDS             complement(7815..9941)
                     /gene="cdhC"
                     /locus_tag="PC700_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/CO-methylating acetyl-CoA
                     synthase complex subunit beta"
                     /protein_id="HDF2579800.1"
                     /translation="MNLYNIIFTGSEQALGAAQAMLAEAIEKNGKEHKVAFPDTAYSL
                     PCIYAATGQKMNTLGDLEGALEVVKSLINRTHLLEHAFNAGLATALAAEVIEALKYST
                     MDAPYSEPCAGHITDPIIRSLGVPLVTGDIPGVAVVLGECPDAESAAKVIKDYQSKGL
                     LTFLVGKVIDQAIEAGVKMGLELRVIPLGYDVTSVIHVVSVAVRAALIFGGLTPGDLN
                     GLLEYTANRVPAFVNAFGPLSELVVSAGAGAIALGFPVITDQTVLEVPMNLLTQKDYD
                     KIVATSLEARGIKIKVTEIPIPVSFAAAFEGERIRKSDMFAEFGGNRTEAWELVVKKE
                     ATEVEDHKIEIIGPNIDEVDADGVLRLPLAVIVKIAGKNMQEDFEPVLERRFHYFLNY
                     IEGVMHVGQRDMAWVRISKDAFDKGFRLEHIGEVLYAKMLDEFESVVDKCEITIITDA
                     EKVSELKGEAIAKYNARDERLASLVDESVDTFYSCNLCQSFAPAHVCVVTPERLGLCG
                     AVSWLDAKATKELDPTGPCQPIEKGECLDDRTGVWNSVNETVNQISQGAVESVTLYSI
                     LEDPMTSCGCFECICGIMPEANGFVVVNREFASVTPVGMTFGELASMTGGGVQTPGFM
                     GHGRHFISSKKFAYAEGGPERIVWMPKELKDYVADKLNATVKEMTGIENFCDMVCDET
                     IADDSEGVLAFLEEKGHPALAMESVM"
     gene            complement(9998..10804)
                     /locus_tag="PC700_000475"
     CDS             complement(9998..10804)
                     /locus_tag="PC700_000475"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDF2579801.1"
                     /translation="MEKFMIIGERIHCISPSIRKALAERDPAPILKRAKEQLEAGAHY
                     IDFNIGPAERDGEEIMTWGIKLLQSEFNNVPIALDTANKKAIEAGLKVYDRTNAKPII
                     NSADAGSRFDLIDIAAEYEAMVIGLCAKEGIPRDNDERMAYCQEILEKGLMLGMEPTD
                     ILFDPLCLVIKGMQEKQVEVLEAIKMMTEMGLLTTGGLSNVSNGCPKHVRPVLDSAFL
                     AMAMANGFSSAIMNPCDPELMKTVKSCDIINGASLYADSFLELNEGGFAF"
     gene            complement(10854..12221)
                     /locus_tag="PC700_000476"
     CDS             complement(10854..12221)
                     /locus_tag="PC700_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HDF2579802.1"
                     /translation="MALKALDIFKLTPKKNCKDCGFPTCMAFSMKVASGAVEVGKCPH
                     MSDDAIAKLSEATAPLMKALKVGAGASEYELGGETVLFRHEKTLVSRNRYAVSFCTCM
                     SDEAVDAKIANMKKVDYVRIGEQMKVEMAVLEYCGDKDAYLKLIDKIKGSGLEVAYIL
                     ACDDAQVVKEAVEVLKDARPMVYGATKENYKDMIEVVKGASLPLGVKAGSLEELYETV
                     ELIQAAGYKELVLDVTGENIKDTYTNAIQVRRTALKEQDRTFGYPSIVFANRLSNSNP
                     MMEVALSSIFTIKYGSIIVIDDISYAKALPLFALRQNIYTDPQRPMRVEPKIYPINNP
                     DENSPVLVTVDFALTYFIVAGDIERSKVPVWLVIPDAGGYSVLTSWAAGKFGGNSISA
                     FIKESKVEEVTNCKDLIIPGKVAVLKGDIEDNLPGWNVVIGPEESMELPKFLKGYQEK
                     ACQTN"
     gene            complement(12252..13196)
                     /locus_tag="PC700_000477"
     CDS             complement(12252..13196)
                     /locus_tag="PC700_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HDF2579803.1"
                     /translation="MAFKMSTQKYSGKISEVEVGIGEKAIKLGGENVLPFYSFDGEVG
                     NSPKIGIQISDVYPESWTDSYKELYKDVANCPVEWAKYVEANTQADFICLKFDGSDPN
                     GLDKSVDECADVAKAVIEAIKLPLVVAGSGNHEKDGKLFEKLAQTLDGHNCLFMSAVE
                     DNYKGVGASAGMAYAHKVGAESSVDINLAKQLNVLLTQLGVKGENIVMNVGCSAVGYG
                     YEYVASTMDRIRLAAFGQNDKTLQMPIITPVAFEVGHVKEAIAPIEDEPDWGCPEERT
                     IAMEVSTAASVLVGGSNAVILRHPKSIETIKELVNALA"
     gene            complement(13216..13986)
                     /locus_tag="PC700_000478"
     CDS             complement(13216..13986)
                     /locus_tag="PC700_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2579804.1"
                     /translation="MGYNIAVAGKGGTGKTSLTGLLIDYLVKDKKGPVLVVDADANAN
                     INEVLGIEVEATIGEIREEVNQREKLGNAFPGGMTKAQYLQFRLNSIIEEGEGYDLLV
                     MGRSEGEGCYCFVNGILREQVNKISGHYKYLVMDNEAGMEHLSRKVTRHVDTLLLVSD
                     CSRRSIQAVARIRDLAEELKLSVGRILLIVNKVPNGVMNDGVKEEIEKHNLELIGVVP
                     MDELIYEYDSTGIPLVNLPEDSKSKVAMKEIFAKLELK"
     gene            complement(14042..15427)
                     /gene="lpdA"
                     /locus_tag="PC700_000479"
     CDS             complement(14042..15427)
                     /gene="lpdA"
                     /locus_tag="PC700_000479"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDF2579805.1"
                     /translation="MKIVVVGGGPGGYVAAIKASMLGADVTVIEKRRVGGTCLNAGCI
                     PTKALLASSGVLNTVKEAKDFGIEIDGTVKPNFTAIMERKNKVVNQLISGIEFLFEKR
                     GVNLVNGFGKLIDKNTIEVTKDDGTVETIKADKIILANGSVPVVPRMFPYDGKVVITS
                     DEVLGLEEIPESMLIVGGGVIGCEIGQFFRALGTEVTIVEMVDQILLNEDKDVAKQLL
                     RQFKKDKIKVITGIGVQTCEVVDGKAVATLSNGKVIEAQYALVCVGRRPNLDNSGVED
                     IGIEMERGKVVVNEHLETNVEGIYAIGDIIDTPFLAHVASKEGIVAVENALGKTKVVD
                     YRAIPRCVYTEPEVAGVGKTEKQLEAEGVEYNVGQFDFRGLGKAQAIGHFQGFVKVIA
                     DKETDKIIGAAVVGPHATDLLTELSLAVHLGLTVEQVGDAIHPHPSLSEGLMEALHDV
                     HGECVHSVPKL"
     gene            complement(15471..16352)
                     /locus_tag="PC700_000480"
     CDS             complement(15471..16352)
                     /locus_tag="PC700_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HDF2579806.1"
                     /translation="MSLLRETLESGKFAVTTEMAPPKGTDLSHLIECAKPLVGRVHAA
                     NVTDFQSAVMRATSLATCKLLKDAGLEPVIQITGRDRNRIAIQGEMLSAGVFGINNLL
                     ALTGDHTSVGDHPQAKGVFDLDSVGILQTAETLMAGTDMAGNKLKGSPDFYLGASVTP
                     EYSPIEVQLLKMQKKIKAGAKFFQTQALYDINTMRKFRELTRDMDCKVLAGIVPLKSP
                     GMAKFMTANVPGIFVPDEQIERLRAAGKENWVSEGIKMAGELIKQLKEEDLCDGVHIM
                     AIGAEENIPAILDAAGL"
     gene            complement(16388..17062)
                     /locus_tag="PC700_000481"
     CDS             complement(16388..17062)
                     /locus_tag="PC700_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HDF2579807.1"
                     /translation="MIISENKPLEEVLGYLKDFDKLVLVGCNQCAATCKSGGEEEVLK
                     MKETLEGEGKKILGYVMLDPACNLLKSKKDLKALKEETKEADAVLSLACGDGTQTIVK
                     NLKDKPVYPANNTLFIGEVQRVGEYEEACKACGDCELGWTGGICPVTMCAKGLMNGAC
                     GGAKNGKCEVNSENDCAWIKIYERLEAIGQLDNLAEIRPPKDYSKQNNPRSLSAKKKK
                     EAAANS"
     gene            complement(17099..17971)
                     /locus_tag="PC700_000482"
     CDS             complement(17099..17971)
                     /locus_tag="PC700_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HDF2579808.1"
                     /translation="MEGMSTKGQIIKGKPVADKISEELIKEVDLLVKEGINPKLTIVR
                     VGARSDDLSYERGALKRCQNIGITTEVLELAEDITQEEYIDVLKRVNDDKNVNGILCF
                     RPLPKHLNEEVIKYVIAPEKDVDCFSPINSAKVMEGDKSGFPPCTPTAVVEILKHYNV
                     DLKGSKVTVLGRSMVVGKPVSMLLLSEHATVTICHSKTKNLSGVAAEADVLIAAIGRA
                     KMVDESFVKDGAVVIDVGINVDEEGNLCGDVDTNAVLDKVSMITPVPAGVGSVTTSIL
                     AKHVVKACKLQNNK"
     gene            complement(18063..18695)
                     /locus_tag="PC700_000483"
     CDS             complement(18063..18695)
                     /locus_tag="PC700_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HDF2579809.1"
                     /translation="MKIADKTCVDFVEVLSSKEAVPGGGGAAALVGAIGMALGSMVCN
                     LTIGKKKYAEYEESVKEILSKAGKLEKDLLKMIDDDAECFLPLSKAYGLPKETEEEKR
                     IKAETMEKALKVACEVPLNIVRVCYEAIKLHEDLVDKGSRLAISDVGVGVQCLRAAIL
                     GGQLNVVININSIQDKEYVNKVKIEVDKLVEDGVKICDEVYAKVEKALGK"
     gene            complement(18800..20476)
                     /locus_tag="PC700_000484"
     CDS             complement(18800..20476)
                     /locus_tag="PC700_000484"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HDF2579810.1"
                     /translation="MGFKSDIEIAQEAKPQDIREVAKKLGLGEDDVELYGKYKAKVDY
                     NLLKRETGKKAKLILTTAINPTPAGEGKTTTTIGVADAFAKLDKNVLVALREPSLGPV
                     FGVKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAANNLLAAMLDNHIHQGNALRIDPK
                     KITWRRCVDMNDRQLRNIVDGMGKKGDGAVRQDGFDITVASEIMAAFCLASDISDLKE
                     RLGNIIVGYSYEGEPVTARQLKANGAMAALLKDALKPNLVQTLEGTPSFVHGGPFANI
                     AHGCNSVIATRMAMHFADYVITEAGFGADLGAEKFLDIKCRMANLKPDAVIIVATVRA
                     LKYNGGVAKADLNNENLEALEKGLPNLLKHVENITQVYGLPAVVAINRFPLDTEAELK
                     LVEDKCKELGVNVALSEVWAKGGEGGIAVAKEVLRLLDEEENNFRFCYEDDLSIKDKI
                     NAIATKIYGADGVDYTPEADKEIANLEKLGFTKVPVCMAKTQYSLTDDQTKLGRPTGF
                     RITVRQATISAGAGFIVALTGEIMKMPGLPKVPAAEKIDVDENGVIAGLF"
     gene            complement(20725..21504)
                     /locus_tag="PC700_000485"
     CDS             complement(20725..21504)
                     /locus_tag="PC700_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2579811.1"
                     /translation="MKIAITGKGGVGKTTFSSMLSRMFAEDGYRVVAVDADPDANLAL
                     ALGFPKEVYESIVPISEMKKLVSDRTAASVGSFGKMFKMNPKVDDIPENFCKEYNGVR
                     LLTLGTVDSGGTGCVCPEHVLLKRLCSHLILQNKDVVVMDMEAGIEHLGRGTAQGVDA
                     FIVVVEPGERSLQTYRKVKKLGHDIGVNKVFVVGNKIRNKEDEEFIIQNLEDGESLGF
                     IYYNQDVIDSDRANQSPYDSSETTKEQIKAIKDKLMSLKDK"
     gene            complement(21814..23733)
                     /gene="cooS"
                     /locus_tag="PC700_000486"
     CDS             complement(21814..23733)
                     /gene="cooS"
                     /locus_tag="PC700_000486"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HDF2579812.1"
                     /translation="MDEKMLTIDLNSQKMIAKAREEGVETMYDRKEGFKAQCGFGLQG
                     VCCRICGMGPCRISPKTPRGLCGADEHTIVGRNFARMVAGGTAAHSDHARDIAHTLAL
                     ADPNGNYKIRDEAKLITLAKEWDVETEGRDIYDVAHEVAEIALMEFGKPFGTLRFIKN
                     APEPRQKIWKEYAIEPRAIDREIATIMHSTHIGCTGDIDSLIHMSLRTSMADGWGGSM
                     IGTRLSDILFGTPVPRRTEANLAVLEENKVNIILHGHEPALSEMIVLASEEPDLVALA
                     KEVGADGINLAGMCCTGNEITMRHGVKIAGDFHQQELAIITGAVEAVIVDVQCIFPAL
                     ARVADCYHTKFVTTSPKAKITGSTYIEFREEQALDDAKAIVKEAILNFKNRDKSKVLI
                     PELKSGATVGYSVEAVVNQLDRVVNSHIDPAGTVKPLTDCLKAGVLRGAAGVVGCNNA
                     KGVSNEAHVTIMKELIKNDIIVVTTGCGASAAAKFGLMESDAAEKYAGKGLATVCKLV
                     GIPPVLHMGSCVDISRILDLVGAAANYLDMDMCDLPVVGIAPEWMSEKAVAIGCYVVA
                     SGIDTYLGIMPPIAGSSRAVDILTSELKDKVGATFTVNTNPKELAATIIEDIEKKRVH
                     FEALVEEKMAEKAEA"
     gene            complement(24340..25251)
                     /locus_tag="PC700_000487"
     CDS             complement(24340..25251)
                     /locus_tag="PC700_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HDF2579813.1"
                     /translation="MIKKLDDILEQLKGGEKIVLSVAAAHDKEVLMAIKDAVERNIIT
                     PILVGNEGKIREISKEIGFDLSGIKIVDKDDIKECAEIAVKLVSSKEADFVMKGLLDT
                     SVILKEVLNKDYGLRTDSLLSHVMVYELEKYHKLLITTDGGMNIAPDYEQKAKILKNS
                     IKAAKALGMETVKVACLAAKEKVNPKMQATVDADMLAKACKEGEFGENVIVEGPLAFD
                     LAVSKEASEIKGFKSEVSGDVDIILVPTIEVGNGIGKAFTYMADSKSAGIIMGAKAPI
                     VLVSRADSHESKLYSIAYGAIVAKNMK"
     gene            complement(25367..25489)
                     /locus_tag="PC700_000488"
                     /pseudo
     CDS             complement(25367..25489)
                     /locus_tag="PC700_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902116.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
     gene            complement(25572..26714)
                     /locus_tag="PC700_000489"
     CDS             complement(25572..26714)
                     /locus_tag="PC700_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF2579814.1"
                     /translation="MDFKIEKYLLKKAEELAFITIKHGGEFKLKSYKVPKGGLDVPIK
                     NEVLVKGIKEKTAQDKLNSMSIADAMIYIIGIDSKFKNNAEYEKFLNALSKDIDLDLK
                     SYMGYMSRKYFEIGEHTDSLIYIKAFITMYPDDLDAMYNYAIVCQEIAKQYQKDMDDK
                     AMNAFLLEAMAKLEKVIDVDENFALGYYHLGYHYYNQGQYLKTKLTWEEALRLGLDAD
                     LVAEVQENLGKMDFKVQYEEGYTLVFQGKFKEGLEKLLPLEEEHMDWWNLLFMIALGY
                     KGMGEIEQAKMYLEKILIIKPNQVDTIVELGLCEAYKNNLDKAIEYFEQAAKIKEDPE
                     ILCNLGMAYLNNGDIDDATYYIERAYELNPQDEITIACLRELGINK"
     gene            complement(26732..28486)
                     /locus_tag="PC700_000490"
     CDS             complement(26732..28486)
                     /locus_tag="PC700_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HDF2579815.1"
                     /translation="MKILLTTLNSKFIHTNLAIRYLKEFVRDLIEVNMKEYTINNDLD
                     YILKDIYKNEYDIILFSTYIWNVGDIVKLCDNLKKIRPNTKIALGGPEVSYDSYEAMK
                     KYDFVDYILYGEGELIFRDLVLHLQGKMKINDVNGLVYRQGNEIIVNKPMELLQDLDE
                     IPSPYENLNPKEYENRIVYYESSRGCPFNCQYCLSSTIPGLRYFSLDRIKSDLKALID
                     ARVSQIKFIDRTFNANRKVAMEIMDFLMKNDNGYTTYHFEVTAYLIDDKMLEFLADCK
                     EGLFQFEIGVQSTNEKTLDAVGRRDDFKKLSHVVQTVASYRNIHQHLDLIAGLPYEDY
                     KSFENSFNDVFNLGIEHLQLGFLKMIKGTGMRKVADEHGFKYKDYAPYEFLYNNYISY
                     EETLKLKDIEDILERYYNSKNFVLSMRYIIGRFYKQSPFKFFEVFAKYFDENGYFDLA
                     QGKNQLYKILMDFYNEKINIDNDVFNDILKYDYISLGKTSNIPQFFNKLDVDDFKNRC
                     HVFLQDNDNLSTYLPSFVDKPAKHIIKYVHFEPFRFNVVDLKNDINTEIREVENVVLF
                     EYDDKKIFEKSKTHKVEI"
     gene            28622..28828
                     /locus_tag="PC700_000491"
     CDS             28622..28828
                     /locus_tag="PC700_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2579816.1"
                     /translation="MELIEKVSINSLSKRDLLLIIKSLEYTNENTQISDFIDLRNNIV
                     KELCFLTDTKEKDFLDYLEKNSTL"
     gene            complement(28833..29756)
                     /locus_tag="PC700_000492"
     CDS             complement(28833..29756)
                     /locus_tag="PC700_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2579817.1"
                     /translation="MNDELLKKVYTSSFSSASRALKDLTIINTKSLIDDKTQRCVYID
                     ENRLRDELIYYRFYGEKIGNINFLNILLPLILSNTNIQKSEDEVIKLIKKYVIYFKKE
                     ELLFDYLLGSVVYNSIMHNLINNSKIEYVELLQSIKDKIIGFSIELDKSDVVKFQMAR
                     IKAIQLIDKYIDLKSEDYDEESILLNVLNVLYDVYMEDRTVENEGINSIKKSILSILG
                     EDSKLNEDNIDFIFSMSEYVVKLRKYKIGVKAYNKSIDPRSLIRLEEGNTIIDPIFNQ
                     ITVMSKTFNDNILSIKINSKSGIYILKFKKV"
     gene            complement(30010..31710)
                     /locus_tag="PC700_000493"
     CDS             complement(30010..31710)
                     /locus_tag="PC700_000493"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDF2579818.1"
                     /translation="MQDFKVAISNCLKEKIEDLSKEEIEALIEVPPNKDMGDYAFPCF
                     KLAKVFRKAPNMIASELAESIEPSGEITKVIQLGGYVNFFVNKSQLAETVIKKVLDEK
                     ENYGHSDFGKDKTVIVEYSSPNIAKPFHIGHIRTTVIGNALYKIYDSQGYKTIRINHL
                     GDYGTQFGKLIVAFKKWGEKEVVESNPIPELLKLYVRFHDEAEQHPEMEDEARAWFNK
                     LENGDEEAQELWQWFRNESLKEFNRVYKLLDIEFDSLAGESFYSDKMNRVIELLEEKN
                     LLKESKGARIVDLEEYKMPPALITKNDGSTLYMTRDLAAAIYRKETYDFDKCIYVVGS
                     QQNLHFQQWFKVIELMGYDWAKDLIHVGFGMVALEEGTMSTRKGRVVFLEDALNQAID
                     KTKEIILAKNPNAKNVDEISKQVGVGAVVFQELSNSRIKDYTFSWERTLSFDGETGPY
                     VQYTHARCCAVLRKAEVEVTSDIDYSLLADEDSAEVLRVIESFNKNILLALKKNEPHI
                     VTRFMLDLAQAFNKFYHDNPILVENLEIRKARLALVLATKQTLENSLKLLGMHAPERM
                     "
     gene            complement(32121..32534)
                     /locus_tag="PC700_000494"
     CDS             complement(32121..32534)
                     /locus_tag="PC700_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HDF2579819.1"
                     /translation="MILVSACLIGINCKYSGDNNENEKVKEYLKGKEFTLVCPEQLGG
                     LSTPRPPAEIVDGKVITKDNKDVTENFVRGANETLKIANLYVCSEAILKEGSPSCGCN
                     LIYDGSFSGKKIPGKGITARLLEEEGIKIKSEKDL"
     gene            complement(32602..34980)
                     /locus_tag="PC700_000495"
     CDS             complement(32602..34980)
                     /locus_tag="PC700_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDF2579820.1"
                     /translation="MNEKSLRVLEYNKIIDLLKKKASSSLGLKYIENLVPNTDFVEVK
                     SMLEETSEAQSIIIKRGSVGLEGIHDIEDKVKRAYIGASLDPGSLIMIADTLRVARRL
                     RNSLSSSDEEDFNYPIIQSLSNSLYVYKDIEDQIYNAIISEVEISDNASSTLRDIRRR
                     IAQKNQSIRSKLNSIISSTTYQKYLQDAIISLRGDRFVVPVKSEYRSQVAGIVHDQSS
                     SGATLFIEPMTIVEMNNELRQLKLGEQEEIERILSEISAMVGEVSEDLISNQEILGRL
                     DFAFSKGKLSIQMRGIEPTLNEDKYLNIKNGRHPLLDKKKVVANTIYLGRDFHTLVIT
                     GPNTGGKTVTIKTVGLFALMTQSGLHIPADYGSSMCVYDNVFADIGDEQSIEQSLSTF
                     SSHMTNIVSILQNVTADSLVIFDELGAGTDPVEGAALAIAVLEDINSVGAKCIATTHY
                     SELKNYALTKPGVENAAVEFDIETLSPTYKLLIGVPGKSNAFEISRKLGLSDYVISRA
                     KEYINTENIALEDVLQNVEKNRIKAVEDREEAERLKEEIEKLKVEYDEKLEKLVSQRD
                     KMIEKAKSEAFSIIRQAKEEVDIIIKELRNLEQERASKEKNRKIEELRKELTSSMGSL
                     QPTVKSMIVPKVSNKEIKDLKPGEEVKVITLNQNGSVVSVDKKRKEAVVQIGIMKMTL
                     PFKSLQKTRKDVSTNVTKSTRNIIRSKSGSVKNEVDLRGLNLEEAIMEVEKYLDDAYV
                     AGLESVTVIHGIGTGVLKAGLQDILRRNRHVKSQRGGQYGEGGAGVTIVKLK"
     gene            35297..36748
                     /locus_tag="PC700_000496"
     CDS             35297..36748
                     /locus_tag="PC700_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HDF2579821.1"
                     /translation="MGNVLEGLKPESVFKNFEKISQIPRGSGNEKGISDFLLSFGKNL
                     GLETIQDESLNIIIRKPATKGYENCPGVVLQGHMDMVCEKEKNVEHDFLKDPIKLRID
                     GDMIYATGTTLGADNGIAVAMGMAILEDNTLEHPALEVLVTVNEEDGMNGADALDPSL
                     IKGQYILNMDSEEEGYLLVSCAGGKTCVVSLPVEYKEVKGDKQGLLVEVTGLLGGHSG
                     MEIVLQRANANKAIARVLSVLNVDYELASVDGGTKHNAIPREAKCVIAVNKADVESAK
                     KQINDILTAFKHEFTTSDPGMTYSVAETSVDKVLTKDCKEKVVQMSCLTPHGVQSVSL
                     DIEGLVESSTNFAIIETKESTIEFLTSVRSSVMSIRDEIADRIRLLAQALGANYDLIA
                     QYPAWEFKKGSKLEKICSETYEKLTGKVPTVMALHAGLECGLLLDKLPHAEAISIGPD
                     MFDVHTPNEHVSIPSVANVWDYVIEILKSMNQY"
     gene            complement(36952..39465)
                     /locus_tag="PC700_000497"
     CDS             complement(36952..39465)
                     /locus_tag="PC700_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDF2579822.1"
                     /translation="MEDCVVNLKKLLLLIIVITSIFTSCIFSFGINAIGNDNRTIRVA
                     YPIQKGLTEIDEQGNYFGYTYEYLLEIAQYTGWNYEFVQVPGEINESITKLMKMLENG
                     EIDIMGGMLYNEPMQKIYNYAGNSYGVANTVLQALYENTDIDNIMSSQDVKKIRIAVI
                     ENSDTRLKELDEYCKMNLMSPELVYCKTSDEQLEVLKSGKADVLLNVSMNPIEGVRTV
                     AKFSSKPFYFITPKGSTDLTQKLNSAINNIEQKDPYFSTTLYEKYFTPENNQMFLSDE
                     ETNYIKDTEKLKVGVLLNKPPFQNADKDTGELRGISIDLLNHICDKTGLEFELVPVKS
                     QEDLEDMAKKHEIDIVAGMIYDYEYSREHNIAMSRPFLSTQYTMMINNKLNENSLNGK
                     RIALAKGIDYYGQFEENIVWYDNLEECIEAVNKGYADYTYGEGYSMQYYMNQPKYKNV
                     RLVPQTYEAKSICFGIIKKDKQELLNIFNKAIISISSEKMQSIVYQNTTYRQDFSLLY
                     IMQQNPIETIFIVASLLICIIGFMAFGLHTRTSMNEKISLELKKHTQLYEMTSEYFFE
                     YDYKQDKLIISMPEKGIETYYGLDKSDKESENGNIENERDKIRREYNKKFFDVIKSQE
                     NGTTEMYSIMLDDTFHWIRITSKIIYNDRKIPMYVIGKIANIDSEKEEKNRLLDKVQR
                     DSLTNIYNAAYIRKLTTQNLLNLSTEKNGALLIIDIDYFKSINDTYGHLVGDKVLIDV
                     AKVLDESFAKDDIVGRLGGDEFSVYINDIINKEELINKCNDVYNHIHLIKLPNGESLS
                     ISMGVVITKRGQEYDELYQIADNALYDSKRQGRNRFKIV"
     gene            complement(39688..40509)
                     /locus_tag="PC700_000498"
     CDS             complement(39688..40509)
                     /locus_tag="PC700_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2579823.1"
                     /translation="MIDINLEKCVGCGMCESDCLVNAIKVKDDKAKVKNILCINCGHC
                     MAICPTDAIEMQGFDKNEVIEYNRESFELEPEKLLNFIKFRRSIRQYKDIEVEEEKIK
                     NIVEAGRYTPTGGNRQPIRYILVKEKLKEVKELAIQGLYNLALDTDDNDPVRSIYKNT
                     FKKMYKRYKENGNDSLFYDAPLLMIVVGDMSLGGSAYVDGGLAASNMELMAYSQGLGI
                     CYNGFFVMASNVEPKIKELLGMSENEAVITSFILGYPDVKYKRTVNRNTAKFEMR"
     gene            complement(41028..41504)
                     /locus_tag="PC700_000499"
     CDS             complement(41028..41504)
                     /locus_tag="PC700_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HDF2579824.1"
                     /translation="MKLTTICYIEKDDKTLMLYRNKKKDDIHEGKYVGVGGKFEQGET
                     PEECVIREVKEETGLTLKSLSYKGLITFPKFKDEEDWYMFLYFSDEFEGELSEKDLND
                     CKEGNLIWVGNDKIFDLNMWEGDRLFLNWAKTGNIFSAKIVYDNGKLKDYNVSFLD"
     gene            complement(41538..42116)
                     /locus_tag="PC700_000500"
     CDS             complement(41538..42116)
                     /locus_tag="PC700_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDF2579825.1"
                     /translation="MREEALEILFKYLETDRMRKHCYAVEAVMRELAKRLEPEKEEEW
                     AVAGLLHDLDSDIVGRKPGEICEGHATTTVELLKKENFGDEEMYRAILGHHDGMGVTR
                     TSLMEKAIYAADPITGFITAIALVYPDKKLTSVKTKSVIKRMKETRFASNVNRDAMRS
                     IEDIGIPFDEFAELSLNAMKNISDVLESAENN"
     gene            complement(42220..44838)
                     /locus_tag="PC700_000501"
     CDS             complement(42220..44838)
                     /locus_tag="PC700_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDF2579826.1"
                     /translation="MKKIELLAPVGSFDSLKAAVQNGANAVYLGGKDFSARASANNFD
                     RNELIEAVKYSHIRGVQVFVTVNTLIKQYEIEDFVEYVKFLYDIDIDALIVQDIGMAR
                     LIKKLLPDFELHASTQMVAHSLEDVKYLQSVGFDRVVLARELNVDEIKWICENTTVDI
                     EVFVHGALCVCYSGQCLMSSMIGNRSGNRGRCAQPCRQKYELIDINTGEVVKSNGDYL
                     LSPRDLSTIEDLDKIIETGVLSLKIEGRMKKPEYVATVVSGYREAINEFVDKKKISIS
                     EEIINNLYTIFNRKFTKGYILGEVGKDIMNSNRPNNQGLYIGKVLDYNRKNKRLRVML
                     ENTLKKGDGINLGGGTIGRIIKDGEITTIAHKGDLIELDFVGEAKRNQLIYKTSDSEL
                     MNRVQATFQQDKEFIKTNIRAKVSIKLGKKPELFISDFCGNKSVVEGDKIVEKAIKVA
                     LSKEKIETQIQKLGNTPYKLKEIEIDLDEGVSMPISVLNQMRRDCIELLDEERIKIKD
                     RKFKKNRLKYQPNRVNKQKKLDTVPKIRVKVKNLEQLEAVLNYNVDLIYYEDINTLSK
                     ALQMASSISIDSSISIDSSISIDSSISMDLDTSSSLGIPKELMGSNTNNIDTLSNTSK
                     IDTLNKNHYNTAVTDKKLIYSAPRIVRNKEYKQFEILDNIKGINKVQIGNWGSMEYFK
                     KKDLNIDCYLNAFNSESINHFKEEGASTVCLSQELNLNEIKQTLKYTDVEIEAVIYGY
                     APMMVTEYCPMGVVVRDCKKDKRVAKCKESSYALRDFTDASYRITQDIFCRSTIYNSK
                     VTCMLDNLYELEEIGIDVFRIDFTVEDSEMVKKVIEAHIEVLSNNYKLGKKATDLYKK
                     LDEIGTVAGHYYKGVE"
BASE COUNT        13532 a         8791 c         4978 g        17689 t
ORIGIN      
        1 ttattatttt acttagattc tattgttatt ttacttagaa cttattgtta ttttatttaa
       61 gttctattac tattttcaat catataaatt tacaaaagac tgtacataaa tttattttat
      121 acaaacttat ctacagtctt ttgtgtgaat atatctaaat aatatcgtat ttatttattt
      181 cctctgcaca gtgagaatca gaacctaaaa ctaatggaat atcgtatttt tttaccaact
      241 ctaataagta tccatctata taaggctctt tgcaatactc ttttctattt ccagaaacat
      301 tataatcaag ttcataacct ttttctttaa ttaactttat taagtcttca agaattgtat
      361 ttcctttata atcgtatgga aatacctgat taaactttct aactaaattt aaatgaccta
      421 ttctttttgg tttatgtata cctaaatctg aatttacagc ttttatcaat gtttcataat
      481 acttattata aacagcttca atacttccta atttgtcgat taaattttga aactcctcta
      541 cactgtaatc tacacaataa taatcatcat caattttaat tatatgtact gataaaagag
      601 agtcttctaa ttcctttcca tatttattta aattctcctt tatcccttct tcgtatcctt
      661 ctataaaatc cacttccgac ccaacattta tcttgatttt accctcatac ttttctttca
      721 acttcttgac atcattaaaa tactttggta aatcttcaat actcatacaa ctatcatttt
      781 caggagcagg gtctctaaat ccatttgcat acggaaaatg ttctgtaaat gtcatttctg
      841 ttattccaac atccattgct ctttgtacat atttttcaaa atcatcctta cttccatgag
      901 gacaataagg tgaatgtata tgaccatctt tcataaataa ttcactcctt ttttaaagaa
      961 aaaatctaat ataaatgcat aaatttttta acttcaaaat aatattttta atcatgctaa
     1021 tttttattat atctaatatt agagtaattt taaactgcct tttaagtatc ttatctttag
     1081 tactattttc aacttaacat taatattatt ttttaactta acattaatac tatctttaaa
     1141 tagtaatact caatatataa ttaatcaaat aactcaaatt gtttgatgaa gcttttgcag
     1201 tatttaaaga atatataatt atttaacatt aaataatcca atcttgtaat aaaatttact
     1261 cattaaagta tattacagaa aatatctcaa aataaacttt ttagttcatg atatactctt
     1321 ttttgcatga aattagatat atttttatca tttcaacaat gaaagggagg cttacatcca
     1381 tcttgagata accccttaga taaaattttt aataattaaa catcataaaa tataaaaatc
     1441 gcttatatca aacgtataag cgattaatta tatttacata tgttttttag cctctaacaa
     1501 cattttatta gcttcttcca tatactctat agccttttca atactctttg ctgtttcttc
     1561 ttttccaata gctctagcct tttcaaccca ctctctaaat ccttcttcat gagtttcatt
     1621 gtgatttatc caatgtacta aaagtatttt taaagttttt tcatccttag actctgatga
     1681 ttcctcatga ctatgttcat ggtcgtggtc gtgagcatgg tcatgtggat gttcatgaca
     1741 tatatctcca tgagtatgtt cgtggcagtg gtcatggtca tgatgtccat gatgatatct
     1801 tacaatctct ttatcaaaaa tcattagtta ttccccctgt tattttatat taaactcttt
     1861 ttttataagg tttacagaat cttttacaag tagtgctaat ggtttacctt ctaatccaac
     1921 taactccaca ttttctggca taataggtaa aagtatctta agtgcctcac tgtctgcaat
     1981 agcttcactt atattacaag taacttctcc catcatagaa ttaggtatta ttacagaaat
     2041 aggtgcaact attatatttg cttttttagc tgatactact atagcatttt cccctgtagc
     2101 tcctttatta gcatgagctt tcatcataga actagttgct atagaattag tccctagtgc
     2161 ataaatttct acatatgtag gtaactcttc tctcaaagaa gaaactatct gggctccaat
     2221 acctccaccc attccatcta tcactgctat tatcatatga aattccccta ttaattaatt
     2281 ttcttgtatt attatcttat ggtccatcaa tcttatttct ttaattatcc catctattat
     2341 cttttgctct cctaataaat ccgttaaata aatctttcca tcacaagggt ctatattaac
     2401 cacattttcc ataacctttt ctaactcatt attactttta caaatgaaag ctgatgattc
     2461 acacatataa atctcctcct aatatgattt ttattaaaaa tcaaaaaaag attcccttag
     2521 gtatttcaat actccaaaga aaccttcatg atttcagttt ttgtatatta ttatacctta
     2581 agtatatcac tttaatttat gtaaagtcaa ttaaagttaa cgctttttat tatactcttc
     2641 attcttgtat ttttcatata gttctttaac taattgcttc tcataatcag tcaaatcgcc
     2701 ctccacaaac tcaacattaa attgttcagc taatcccttt actatgtttt tctgtagaat
     2761 atctatgtct atttttctgc caatttcatt ttctattcca cttgcttttt tagctgtaaa
     2821 tttcatagct ctttctttca attctggtgt cttagttttt attaatttaa acaatttttc
     2881 tacatcaaaa tccagtatta ttgaaccatg ttgtaataaa acaccatctt ttctgctttg
     2941 agcacttcca attaccttct tattattaat agtaacttca tatgatgcat gtgcattaaa
     3001 acatgcagct gataaatttt ccctacttat tctctcgcct ctatttaaat tatctgtttc
     3061 tataccactt agattcaatc ctttaactag accttcactt atatatctat aagataggtt
     3121 gatgctctta tcgattaaag gattatcttc tcctataatt atactataag ttagctcatt
     3181 atcatgtaat acagctcttc cacctgtaat tcttctagta tagtctatat tcatacatct
     3241 acacttatct aaatctatct catcttccaa tttctgaaaa tatcctatac tcaaacaagc
     3301 tggttcccat gtataaaatc taagagttgg cttattatgt cctttcttat atgcagtaaa
     3361 tatagcttcg tcaattgcca tattcattgc tccttcatat gatttattat gtataactct
     3421 ccactgattc atgattatta cgcccccatt tttagtataa atatatccta gaaatcctct
     3481 atagatttat ttctcttatt gctaaatcta tctcactttg atttaaaggt cttgaatagt
     3541 tgtatatagt acttccgggt ctttcattat agtatggtct attacaatcc ttacaacctc
     3601 ttatttcaaa aggatagcct ttgtttatgt cttttaaaat atcattgtca agctttatac
     3661 tatttaagta tccattttta aacttaaaat actcttttgg ataaccttta tttatcatat
     3721 aagacattaa ctgtaccctt ctataacttt ctatgcttgg ttgatttatc ttttccattt
     3781 tagttcctct aactggagta aatgcaaata aactaatcgt tacatcattt tcctttaaat
     3841 aaatatataa attgtatata tcttcatgac tttcacccat accaactatt atatgtgtac
     3901 ttattttatt gggatatgat tttgacattt cagttataaa ttttaacttt tcatcatagt
     3961 tatttccttt tattttttca tatagttctt tatttgatgc atctattgct attcctattt
     4021 tatcaactcc tactgaaaaa aacttcttta tctgttcatc attttctaat tttgcagata
     4081 gtgaaattgg catgtctatc ttaccactta tatagtttat aaagtctaaa acagagttat
     4141 gtgcttcttc acttgccatt gattgaatac atattctctt tatatttttt cctttataag
     4201 cttttaaagc atctaatatt tcttcttttg aaaactctgg ccaaattact cttgataatt
     4261 tatcttttct agtactactt tctattgatt gagaacaaaa actgcattta tttatacatt
     4321 tttctccaat cattatatat gctgtggtag gaggtatatc gctttttttg tttaatatcc
     4381 caagctctat tgctgtccca actgatagtc taatcataaa acgcaacact ccttacactc
     4441 atttattgtc atattcattt ctatagcctt attctttgca gaccttgatg gtaatactat
     4501 tctattgact ccacacatta tagataattg gtctagctct tttctataca ctcctcttgg
     4561 tctcatacaa cctagattaa tttcagtatc tggtaaatta attctagctt cacataaaat
     4621 atctgctacc ccttctaact caggaggttc tacattttca tactcagtcc cttttgttgg
     4681 aataaggact ataaatgtaa gcttattagg aggatttttc tgtaggtatt ctataatttc
     4741 atattcaccc tttatttgtc ctccttttaa accaatacat atatgtggtg ctacttctgt
     4801 atgttcttgt attgtattat aaacttcaat ataatcttct ttactctttt tcatcttata
     4861 aacttctctt attgtctcat catcaaacac taaatcaaaa gaaactatat ctaaatactt
     4921 acacaactct acaatgctat ctttatccat aagacctaaa tgagcattca atctatatcc
     4981 tttctctttt aagtttttaa tggtattaat atgagtattt atagggacat ctccctcata
     5041 actacaaccg ccactcaata gaaagctagt tatattcctt gattgtactt tttcctcata
     5101 ttcttctatt ggcaccatat tgtttaagta gtgaccgttg caatgggcac atttcaaact
     5161 acattgattg cttgtagtac taagagctag tgtctggtta ggataagcaa actctatttt
     5221 atttccaaaa ttctttaatt taatatcaaa tgctattttc attttttcag atagattctt
     5281 attcatagtt tattccttct taagtacatt ttactcttct agtaatttct ttgctgttgg
     5341 gaactgctct atattcgtat gtggtaagaa acatgcagaa ataaattcat ccatataact
     5401 tggatataca ctaagttcaa cataagttat ttgattacct atttcttcaa gtttattttt
     5461 tgcatcttta cttatgagag ctaagtaaga accaacaagt gaactattcc ctatataagt
     5521 aaatttttct ctatcaatgt caggaagtaa ccctataatt attgagtttt ctatgtctag
     5581 gttgtttcct ataccacctg ctatataaac cctatctaat atactaaaat ccatacctaa
     5641 actatctaca agagtgtatg cacctgaata tatagctcct ttagctctga tgaaattatc
     5701 tatatcaact tcattgacta ctatatcatt ttctaaatca aattcttctt taaatgctaa
     5761 tacatactct cctatctcat gctcattaaa tctaactctc ttattatcta aatccctgta
     5821 tattttacct cttctatcta ttacaccttt agtaatcatt tgacagatta aatcaattat
     5881 accagaacca catataccaa ctggtgcaca ttcatcaata atctttaatg ttggctctaa
     5941 agtttcttta tcaataatta ctttctctat tgctcctgct gatgccctca ttccacagct
     6001 tatccctcca ccttcaaaag caggtcctgc tgaacatgca cagctcatca tataatcttt
     6061 atttccaaat acaatctctc cattagttcc taaatctata aataaaacat tttcttcact
     6121 tgaccatatc cctgctgata atacacctgc tgttatatct cctcctacat aacttgcaac
     6181 agatggagct aagtacacat atgcactgtc atttacaaaa agaccaacat tttcacccat
     6241 aagttttgga gattttaaaa atggtggtat gtaaggttct tgtcttaaga agtctgtata
     6301 cacacccaag aataaacttg tcatagttgt attcccggca gccactaatg taactacatc
     6361 ttccttatct attccatttc tttcatatat tgtttttaat agtggattta tagtttcctc
     6421 aactatagct ttatgaagtg tctctaatcc atttttctta gttgaatata taattctatt
     6481 tataacatct gctccatatt ttatctgtgc atttccagaa gatgctttat ctactacctc
     6541 ttttgaatat aggtctacta aacaaacaac tactgaagtt gtaccaatat ctattgcaac
     6601 tccgtataag gaattttctt tattaccttg ttctatattt atgattgtaa gtttcttttg
     6661 tttttgaaca tatgttattg taaccttaaa atcactcttt ctaaatacag ttggcatttt
     6721 tctaagtata tccaatctaa aatctatttc attatatcct aggctatttc taacatatct
     6781 ttccaatcta tctacatcac ttatattatc atctaaattt ggttcttcca tttctatgta
     6841 tttctttttt atatttgttt taaattgaag gttatgttct tcaattattt ttttagctct
     6901 ttcaaaaatt tccctatcct cttttttatt actaccttct atcttcatac catgcataga
     6961 agatgatact ttagaaggaa cttcaatttc tatatctgat attacctttg tacaacaagc
     7021 tagtatataa ccttgctccc actcatcatc tgttatatgt cttgtttttt ctgtatcaac
     7081 ttttccattc aataatttta ctttacactt cccacaagac acgtttccat tacaaggagc
     7141 atctatgaat atatctgcat ttcttgcaac ctctagtaat atatcaccct cattacaata
     7201 aacctcttta ttgtttggtg taaaactcac ttttatcatg ttatccctcc caatttgttc
     7261 tatatatgat aaaaggaaag tatagctaaa tttgcgtgca ctcaaatttt actatacttt
     7321 ccccaattat aataatatct taatctttaa ttattctaat ccagcctcgt attctttatc
     7381 acttaataac gtatcaagtt cagccacatc agctagtttt acttttaata tccagttttc
     7441 atatgggtct tcatttatac attctggctc atcttctaat ttttcattaa cttccaatac
     7501 ctcaccatta acaggagata ttaaatcaga agctacttta gaagactcaa ctactccaaa
     7561 atctactccc ttagttacag tatcttctac ttctggtgtt tcaacaaaca atatttctcc
     7621 taattgatct tgagcatagt ctgttatacc tatatataca acatctccat caattacttt
     7681 tacccactca tgatctttag agtattttaa ttctggtaat aatttcattt cgatagaccc
     7741 ccaatattat attattgatt aaattactta aatcatatta atattatatc atattatttt
     7801 aaattttcca tcaattacat tacactttcc atagctaatg ctggatgacc tttttcttct
     7861 aagaaagcta atactccttc agaatcgtca gctatagttt catcacaaac catatcacag
     7921 aagttttcta ttccagtcat ttctttaact gtagcattta atttatcagc tacataatct
     7981 tttaattctt ttggcatcca aactattctt tctggtccac cttctgcata agcgaatttt
     8041 ttagaagata tgaaatgtct tccgtgtccc ataaatcctg gagtttgaac tcctccacct
     8101 gtcatagaag caagttctcc gaaagtcata ccaactggag taacacttgc aaattctctg
     8161 ttaactacaa caaatccgtt tgcttctggc attataccac agatacattc gaagcatcca
     8221 caagaagtca ttgggtcttc tagtatagaa tataatgtaa cagattcaac tgcaccttga
     8281 gatatttggt ttacagtttc atttactgaa ttccatacac ctgttctatc atctaagcac
     8341 tcgccttttt ctattggttg acaaggacct gttgggtcta attctttagt agccttagca
     8401 tctaaccagc taactgctcc acaaagacca agtctttcag gagtaacaac acaaacgtga
     8461 gctggtgcaa atgattgaca taagttacaa gaatagaaag tatcaacact ttcatcaact
     8521 aatgaagcta atctttcatc tctagcattg tacttagcta ttgcttctcc ttttaattca
     8581 gacacttttt cagcatctgt aattatagtt atttcacact tatcaactac tgattcaaat
     8641 tcatctaaca tcttagcata taaaacttct cctatatgct caagtctaaa tcctttatca
     8701 aatgcatctt tagagattct tacccaagcc atatctcttt gtccaacgtg cattacacct
     8761 tctatatagt tcaagaagta gtggaaacgt ctttcaagaa ctggctcgaa atcttcttgc
     8821 atatttttac cagctatttt aactattaca gcaagcggta atcttaatac accatctgca
     8881 tcaacttcat ctatatttgg tcctattatt tctatcttat ggtcttcaac ttctgttgct
     8941 tctttcttaa caacaagctc ccaagcttca gttctgtttc caccaaactc agcaaacata
     9001 tcgctctttc taattctctc accttcaaat gctgctgcaa atgaaactgg gataggtatt
     9061 tcagtaactt ttatttttat tcctctagct tctaatgaag tagctactat cttatcgtaa
     9121 tctttttgag ttaataggtt cattggaact tcaagaactg tttggtcagt tataactggg
     9181 aatcctaaag ctattgctcc agcacctgca gatacaacta attcacttaa tggtccaaat
     9241 gcattaacaa atgcaggaac tctattagct gtgtactcta ataatccgtt taaatcacca
     9301 ggagttaatc caccgaatat taatgctgct cttactgcaa cagatacaac gtgtataaca
     9361 gaagttacat catatcctag aggtataact cttagctcaa gacccatttt tactccagct
     9421 tctatagctt ggtctataac tttaccaact aagaaagtta gtaaaccttt agattggtaa
     9481 tcttttataa cttttgctgc tgattctgca tctggacact caccaagaac aactgcaaca
     9541 ccaggtatat ctcctgtaac taatggtaca ccaagtgatc tgattatagg gtcagttata
     9601 tgacctgcac atggctcact atatggagca tccatagttg aatattttaa tgcttcaatt
     9661 acttctgcag ctaaagccgt agctaaacca gcattaaaag catgttctag taaatgagtt
     9721 ctatttatta aagattttac tacttctaaa gcaccttcta agtcccctaa agtgttcatc
     9781 ttttgtcctg ttgcagcata tatacaaggt aatgaatatg ctgtatcagg gaaagcaact
     9841 ttatgctctt ttccattctt ttctatagct tcagctaaca tagcttgagc cgcacctaaa
     9901 gcttgttctg accctgtaaa gattatatta tatagattca ttctatttcc ccctttaata
     9961 tttacaccta ttttttatta actataccct aaaaacccta gaaagcaaat ccaccttcat
    10021 ttaactctaa gaaagagtct gcatataaag atgccccgtt gattatgtca caagatttta
    10081 cagttttcat taattctggg tcacatggat tcattatagc tgaactaaat ccattagcca
    10141 ttgccattgc taagaatgca ctatctaaaa caggtctaac atgcttagga catccattag
    10201 atacgttaga taatcctcct gtagttaaaa gtcccatctc agtcatcatt tttatagctt
    10261 ctaaaacttc tacttgtttt tcttgcatac cttttataac taagcataat gggtcaaata
    10321 gtatatcagt tggctccatt cctaacatta agcctttttc taatatttct tggcagtaag
    10381 ccatacgctc atcattatct cttgggatac cttcttttgc acataaacct ataaccattg
    10441 cttcgtattc agctgctata tctattaaat caaatcttga gccagcatca gcagagttta
    10501 ttattggttt tgcattagtt ctatcataaa ctttaagtcc agcttcgata gcttttttat
    10561 tagctgtatc taatgctata ggaacattgt tgaactcaga ttgaagtaat ttgattcccc
    10621 atgtcattat ttcttcgcca tctctttcgg caggtcctat attaaaatct atatagtgtg
    10681 ctcctgcttc taattgctct tttgctcttt ttaagattgg agctgggtct ctttctgcta
    10741 atgcttttct tattgaaggt gatatacaat gtattctctc acctataatc ataaattttt
    10801 ccatttataa tgtccccctt taaatttaat attaatagtt agttaatatt ttattagttt
    10861 gtttggcatg ctttttcttg gtatcctttt aagaacttag gtaattccat agattcctct
    10921 ggtcctataa ctacattcca tcctggtaag ttatcttcta tatcaccttt aagtactgca
    10981 acttttcctg gaatgataag gtctttacaa ttagttactt cttctacctt actttcttta
    11041 atgaaagcag agattgagtt tccaccaaat ttaccagcag cccaagatgt aagaactgaa
    11101 tatccacctg catctggtat tactaaccat actggaactt ttgatctttc tatatcacca
    11161 gcaactatga agtaagttaa tgcaaagtca acagtaacta atactggaga attttcatct
    11221 gggttgttga ttggataaat ctttggttca actctcattg gtctttgtgg gtcagtgtat
    11281 atgttttgtc ttagtgcaaa taatggtaat gccttagcat aactaatatc atctattact
    11341 attatagaac cgtattttat agtgaatatt gatgataaag caacttccat catagggttt
    11401 gaattagata atctatttgc aaatactatt gaaggatatc caaatgttct atcttgctct
    11461 tttaaagctg ttcttctaac ttgtatagca ttagtgtaag tatcttttat gttttctcct
    11521 gttacatcta atactaattc tttatatcca gcggcttgaa ttaattcaac tgtttcatat
    11581 aattcttcta agctaccagc ttttacacct aatggtaaac ttgctccttt aactacttct
    11641 atcatatctt tgtagttctc tttagttgct ccatatacca ttggtctagc atcttttaat
    11701 acttcaacag cttctttaac tacttgtgca tcatcacaag ctaaaatata agctacttct
    11761 aatccactgc cttttatttt atctattaat tttaaatacg catctttatc tccacaatac
    11821 tctaaaacag ccatttcaac tttcatttgt tcaccaattc ttacatagtc aacttttttc
    11881 atgttagcta ttttagcatc tactgcttca tcactcatac aagtacagaa tgaaactgca
    11941 tatctatttc tacttactaa tgttttttca tgtctaaata atactgtttc tccacctagt
    12001 tcatactcag atgcaccagc accaactttt aaagctttca ttaaaggtgc agtagcctct
    12061 gataattttg ctatagcgtc atcagacata tgtgggcatt tacctacttc tacagctcct
    12121 gaagcaacct tcatagagaa agccatacaa gttgggaatc cacaatcctt acagtttttc
    12181 tttggtgtta atttaaatat atctaaagct tttaatgcca ttattttttc cccctaacct
    12241 taacaattta attaagctaa tgcgttaacc aattctttta tagtttctat tgattttgga
    12301 tgacgaagta taactgcgtt tgaaccacct actaaaacac ttgctgcagt tgaaacttcc
    12361 attgctatag ttctttcttc tggacaaccc caatctggct catcttctat tggagctata
    12421 gcttctttaa catgacctac ttcaaaagct actggtgtta tgataggcat ttgtaatgtc
    12481 ttatcatttt gaccaaatgc tgcaagtcta attctatcca tagtagatgc aacgtactca
    12541 tatccataac caactgctga acatccaaca ttcataacta tattttcacc tttaacgcct
    12601 agttgagtta acaatacgtt taattgttta gctaggttta tatcaacaga agattcagct
    12661 cctactttat gtgcataagc cataccagct gatgctccta ctcctttgta gttatcttct
    12721 actgctgaca taaataagca gttatgtcca tccaatgttt gagctaattt ttcaaataat
    12781 ttaccatctt tttcatgatt tcctgaacca gctacaacta aaggtaattt tattgcttca
    12841 attactgcct tagctacatc agcacattca tcaactgact tatctaatcc atttgggtca
    12901 gaaccatcaa attttaaaca aataaaatct gcttgtgtat tagcttcaac atatttagcc
    12961 cactctactg gacaattagc tacatctttg tataattcct tatatgaatc agtccagctt
    13021 tcaggataaa cgtctgatat ttgtatacct atttttggag aattacctac ttctccatca
    13081 aaactataaa aaggtaatac attttcccca cctaatttaa ttgctttttc ccctattcct
    13141 acttcaactt ctgatatttt tccagaatat ttttgagtag acattttaaa tgccatattt
    13201 ttttccccct taagcctatt ttaattctaa tttagcaaaa atttctttca tagccacttt
    13261 agattttgaa tcttcaggta aattaactaa aggaatacct gtagaatcat attcatatat
    13321 caattcatcc ataggtacaa caccgattag ctctaggtta tgcttttcaa tttcttcttt
    13381 aacaccatca ttcataaccc catttggaac tttatttaca atcaaaagta ttcttccaac
    13441 acttaacttt agttcctcag ctaaatctct tattctagca acagcttgta tgctacgtct
    13501 tgagcaatca ctaaccaata aaagtgtatc aacatgtcta gtgacttttc tacttagatg
    13561 ttccatacca gcttcgttgt ccatgactaa gtatttgtaa tgaccagata ttttgtttac
    13621 ttgttctcta agtatcccat ttacaaaaca gtaacatcct tcaccttctg acctacccat
    13681 tactaataag tcatatcctt cgccttcttc gattatagaa tttaatctaa attgaagata
    13741 ttgtgccttt gtcataccac cagggaatgc attgcctaac ttttctcttt gatttacttc
    13801 ttctcttatt tctcctattg ttgcttcaac ttcgatacct aacacttcat ttatgttagc
    13861 attggcatca gcatctacaa ctaatactgg tccctttttg tccttaacca aataatcaat
    13921 taaaagccct gtgagacttg ttttaccagt tccacctttt cctgcaacag ctatattgta
    13981 tcccatttta agacttcccc cttaaatcta gccacagagc cttatgtact ctgtctgtct
    14041 attataattt aggtacagaa tgaacacatt ctccatgtac atcatgaagt gcttccatta
    14101 gcccttcaga taaacttgga tgtggatgta ttgcatcccc tacttgttct actgttaatc
    14161 ctagatgaac agcaagtgac aactctgtca ataaatctgt agcatgaggt cctactactg
    14221 ctgcacctat tatcttatca gtttctttgt cagctataac ttttacaaat ccttggaaat
    14281 gtcctatagc ttgtgctttt ccaagacctc taaaatcaaa ttgacctaca ttgtattcta
    14341 caccttcagc ttcaagttgc ttttcagtct taccaacacc agcaacttca ggttcagtat
    14401 aaacacatct tggaattgct ctataatcta ctaccttagt cttacctaaa gcattttcaa
    14461 ctgctacgat accttcttta gaagcaacat gtgctaggaa aggagtatct attatatcgc
    14521 ctattgcgta tataccttct acattagttt caagatgttc attaactact acttttcctc
    14581 tttccatttc aattcctata tcttctactc cagaattgtc aaggttaggt cttctaccaa
    14641 cacatactaa tgcatattgt gcttctataa cctttccatt agaaagagtt gcaaccgcct
    14701 taccatcaac tacttcacaa gtttgtactc caatacctgt aataacctta attttatctt
    14761 tcttaaattg tctaagtagt tgtttagcta catctttgtc ttcatttaat agtatttggt
    14821 caaccatctc aactatagtt acttctgttc ctaaggctct aaagaactgc cctatctcac
    14881 atcctataac tccaccacct actattaaca tagactcagg tatctcttca agtccaagaa
    14941 cttcatcact agttattaca acttttccat catatgggaa cattcttggt acaactggaa
    15001 cagaaccatt tgctagtatt atcttatctg cttttatagt ttctactgtt ccatcatctt
    15061 tagttacttc tatagtgttt ttatcaatta attttccaaa accattaact aagtttactc
    15121 ctcttttttc aaataagaac tctataccac ttattaattg gtttacaact ttattttttc
    15181 tttccattat ggcagtgaag tttggcttaa ctgtaccatc tatttctata ccaaaatcct
    15241 ttgcttcttt tacagtattc aatactcctg aagaagcaag aagtgcttta gttggtatac
    15301 atcctgcatt taagcaagtt cctccaactc ttcttttttc aataaccgtt acatctgcac
    15361 caagcataga agcttttata gctgctacat atcctcctgg tccaccacct actactacta
    15421 tcttcatcct taattccccc aaacctaata taatctacaa tcttgcaata ctataaacct
    15481 gcggcatcca gtatagcagg tatattctct tctgccccaa ttgccattat atgaacacca
    15541 tcacataaat cttcttcttt taattgtttg ataagttccc ctgccatttt tattccttca
    15601 cttacccagt tttcttttcc agctgctctt aatctttcga tttgttcatc tggaacgaat
    15661 attcctggta catttgcagt cataaattta gccattcctg gtgattttaa aggtactatt
    15721 cctgctaata ccttgcaatc catatccctt gttaattctc taaactttct cattgtattt
    15781 atgtcataaa gtgcttgtgt ttggaaaaat ttagcccctg cttttatctt cttttgcatt
    15841 tttaataatt gaacttctat tggagaatat tctggagtta ctgatgctcc cagataaaaa
    15901 tctggagaac cttttaactt attaccagcc atgtccgtac ctgccattaa agtctcagca
    15961 gtttgaagta tccccacaga atctaaatca aacactccct tagcctgtgg atggtctcct
    16021 acgctagtat ggtctcctgt tagtgccaac aaattgttta tcccaaatac acctgctgac
    16081 aacatttctc cttgtatagc tattctattt ctatctctcc ctgttatttg aataactggc
    16141 tctaatccag catcttttaa taatttacat gtagcaagag aagttgctct cataactgcc
    16201 gattgaaagt ctgttacatt tgctgcatga actcttccaa ctaacggctt tgcacattct
    16261 attaaatgtg aaaggtcagt cccttttgga ggagccattt cagtagtaac tgcaaatttt
    16321 ccactttcta atgtttctct taataagctc atctctaatc ccccttttaa ccttctaaaa
    16381 aaaactttta tgaatttgct gctgcttctt tctttttctt tgcacttaaa cttcttggat
    16441 tattttgctt agaatagtct tttggtggtc ttatttctgc taagttatct aattgtccta
    16501 tagcttctag tctttcatat atctttatcc aagcacaatc attctctgag tttacttcac
    16561 acttaccatt tttagctcca ccacaagccc cattcataag acctttagca cacatagtta
    16621 ctggacatat cccacctgtc caaccaagtt cacagtctcc acaagcctta caggcttctt
    16681 catattctcc aactctttgt acttccccta taaacaaagt attgttggct ggataaactg
    16741 gcttatcttt taaattctta actattgtct gtgtaccatc tccacatgct aaagataaaa
    16801 ctgcatcagc ttccttagtc tcttctttta gagcctttaa atccttttta gactttaaaa
    16861 ggttacaagc agggtcaagc attacatatc ctaagatttt tttgccttca ccttcaagag
    16921 tttccttcat ttttaaaact tcttcttccc caccactttt acaagttgct gcacattgat
    16981 tacaaccaac taaaaccagc ttgtcaaaat cctttaagta ccctagcact tcttctaatg
    17041 gtttattttc agaaattatc atatctactt cccccctaaa ccttccacat atatatctct
    17101 atttattgtt ttgtaattta caagccttaa ctacatgttt tgcaagtata gatgttgtaa
    17161 ctgaaccaac ccccgctgga actggagtta tcattgatac tttgtctaaa actgcatttg
    17221 tatcaacatc tccacataaa tttccttctt catcaacatt tataccaacg tctataacta
    17281 ccgctccatc tttaacaaaa ctttcatcaa ccatcttagc tctaccaata gctgctatta
    17341 gcacgtctgc ctctgctgct acacctgata gatttttagt ttttgaatga catattgtaa
    17401 cagttgcatg ttcacttaat aaaagcattg atactggctt tccaactacc attgatcttc
    17461 caagaactgt taccttactt ccttttaaat caacattata gtgttttaat atctcaacta
    17521 ctgctgtagg tgtacatggt ggaaatccac ttttatcccc ttccattacc ttagcagagt
    17581 ttattggact aaaacaatct acgtcttttt ccggtgcaat aacatatttt ataacttctt
    17641 catttaagtg ttttggaaga ggtctaaaac ataatatacc attaacattt ttgtcatcat
    17701 taactctctt taatacatca atatattcct cttgtgttat atcctcagct aactctaaca
    17761 cttctgttgt tatacctatg ttttgacatc tctttagtgc tcctctttca taagataaat
    17821 catcacttct tgccccaact ctaactattg ttagtttagg atttatacct tctttaacta
    17881 gtaaatcgac ttcttttatt aattcttcac taattttatc agctacaggt tttcctttta
    17941 taatttgtcc tttagttgac attccttcca tacattcaca actcccctac aagcaaactt
    18001 aaattaattt attctatata ccacatatta ataacatagt atctactttc gtcatttaaa
    18061 aattattttc caagagcttt ttcaactttg gcataaactt catcacaaat ttttacacca
    18121 tcttcaacta acttatctac ttctattttt accttattaa catattcttt atcttgtata
    18181 gaatttatat ttataactac atttaattgt ccaccaagta tagcagctct taaacactga
    18241 actcctacac caacatcact tatagcaagt cttgaacctt tatcaactaa atcttcatgt
    18301 aacttaatag cttcatagca aactcttact atgtttaatg gtacttcaca agcaactttt
    18361 aatgcctttt ccattgtttc tgcttttatt cttttttctt cttctgtttc tttaggaagc
    18421 ccgtaagctt ttgataatgg taagaaacat tctgcatcat catctatcat ttttaataaa
    18481 tctttttcta actttcctgc cttacttaat atctctttta cgctttcttc atactcagca
    18541 tacttcttct tccctattgt aaggttacat accatactac ctaaagccat acctattgca
    18601 cctactagtg cagcagctcc cccaccacct ggtactgctt ctttggaaga tagtacttct
    18661 acaaaatcaa cacaagtttt atctgctatt ttcatgataa tcctccttga aaggatgtag
    18721 gaaaatatct caaattattc gagatatttt acgaactatt ttaagatatc tcctactctt
    18781 tatttataaa ttaatttcct tagaataatc cagctattac tccattttca tcaacatcta
    18841 ttttttctgc tgctggaact tttggtaatc ctggcatctt cattatttct ccagtaagtg
    18901 caactataaa tccagctcct gctgaaattg ttgcttgtct tacagttatt ctaaatcctg
    18961 ttggtctacc taatttagtt tggtcatcag ttaaagaata ttgagtctta gccatacata
    19021 ctggaacttt agtaaatcca agtttttcta agttagctat ttccttatca gcttcaggag
    19081 tataatctac accatctgct ccataaatct tagtagctat agcatttatc ttgtctttta
    19141 tagataaatc atcttcatag cagaatctaa agttattttc ttcttcatcc aataatctta
    19201 aaacttcttt agctactgca attccacctt ctccaccttt tgcccatact tcagaaagag
    19261 caacattaac acctaattcc ttacacttgt cttcaacaag tttaagttca gcttcagtat
    19321 caagtgggaa tctattgatt gcaacaactg ctggtaatcc atatacttga gttatgtttt
    19381 caacatgttt taataagttt ggaagtcctt tttcaagagc ttctaggttt tcgttattta
    19441 agtctgcttt agcaactcca ccattgtatt ttaatgctct tacagtagca actattataa
    19501 cagcatctgg ttttaagttt gccattctac attttatatc taagaatttt tctgctccaa
    19561 ggtcagcacc gaaaccagcc tcagttatta catagtctgc aaagtgcata gccattcttg
    19621 tagctataac agagttacat ccatgagcta tatttgcgaa tggtccaccg tgtacaaatg
    19681 atggtgttcc ttcaagagtt tgaactaggt ttggttttaa agcatctttt aataatgcag
    19741 ccatagcacc attagctttt aattgtctag ctgttacagg ttcaccttca tagctgtatc
    19801 caactattat gtttcctaat ctttctttta aatcagatat atcacttgcc aaacagaacg
    19861 ctgccattat ttcagaagca acagttatat cgaatccatc ttgtctaact gctccatcac
    19921 cttttttacc cataccatca acgatatttc taagttgtct atcgttcatg tccacacatc
    19981 ttctccaagt tattttctta gggtctattc taagtgcatt tccttggtga atatggttat
    20041 caagcatagc agctaaaaga ttgttagcag ctcctatagc gtgaaaatct cctgtgaagt
    20101 gtaagtttat atcttccata ggaacaactt gtgcatatcc tccaccagct gctccacctt
    20161 taacaccaaa tactggtcct agagatggtt ctcttaaagc aactaataca tttttgtcta
    20221 gtttagcaaa tgcatcagca acacctatag tagtagtagt ttttccttca cctgctggag
    20281 ttggatttat agctgtagtt agtattaact tagctttttt accagtctct ctttttagta
    20341 gattgtagtc aacttttgct ttgtatttac cataaagttc tacatcatct tcacctagtc
    20401 ctaatttttt tgcaacctct ctaatatctt gaggcttagc ttcttgagct atttcaatgt
    20461 cagatttaaa tcccataact gaatctcctc ccacaaccta aatagattta aaatataatt
    20521 tttgtaaaaa ttgtattaat atgtaaaaat tatattttat aagataatta tacatgtaaa
    20581 attacttaaa aactagctac taagctatta aaatattcaa acttaatagc tagttattta
    20641 tctaattact tacttattac ctatctattg cctatgtcta acctttaacc aacttaaaat
    20701 cccaaatcaa aatttctatc tattttattt atccttaagt gacattaatt tatcttttat
    20761 tgctttaatc tgttctttcg ttgtttcact agaatcatat ggagattgat tagctctatc
    20821 agaatcaata acatcttggt tatagtaaat aaatcctaaa gattctccat cttctaagtt
    20881 ttggattata aattcttcat cttccttatt tctaatttta tttcctacta caaatacttt
    20941 atttacacct atatcatgtc caagtttctt aacttttcta taagtctgta gacttctctc
    21001 tcctggttct acaactacta taaaagcatc tacaccttgg gctgttcctc ttcctaagtg
    21061 ttcaattccc gcttccatat ccataaccac tacatcttta ttttgtagta ttaaatgtga
    21121 gcagagtctc ttcaaaagaa catgctctgg acatacacat ccagtaccac cagaatctac
    21181 ggttcccaat gtaagtaatc ttactccatt gtattcttta cagaaatttt cagggatatc
    21241 gtctacttta ggattcatct taaacatttt tccaaatgaa cctacagaag cagctgttct
    21301 atctgaaact aattttttca tttctgatat aggtactatt gattcatata cttcttttgg
    21361 aaaacctagt gctaaagcta aattagcatc agggtcggca tcaacagcaa caactctgta
    21421 tccgtcttca gcaaacatcc tagagagcat tgaagaaaaa gtcgttttac ctactccacc
    21481 tttacctgtt attgctatct tcataatccc taccaccttt tattactatt tgctttttag
    21541 tagttaattt tacagttaaa tattttttat actctatatt tttatacact aaaaataaac
    21601 tacagctagt ccttacagga accaattaaa tttaccctac tagcttttca aagcagcatg
    21661 tatagtttta ttattcttta atattgttta tattttattt attcagtata ttcattatat
    21721 ttcgatgttc tttactttaa tatttgactt tattcaactt tactcaacca gaaacttctt
    21781 ataaaaacgt ttattctata acttcactat tttttaagct tcagcctttt ctgccatttt
    21841 ttcttcaact aatgcttcaa agtgaacacg ttttttctct atatcttcta ttattgtagc
    21901 tgctaactct tttggatttg tatttacagt aaatgtagct ccaactttat cttttaactc
    21961 actagttaat atatccactg ctcttgaaga accagctatt ggaggcatta ttcctaaata
    22021 agtgtctata ccagaagcta caacatagca tcctatagca actgcttttt ctgacatcca
    22081 ttctggagct atacctacaa ctggaaggtc acacatatcc atatctaagt aattagctgc
    22141 tgctccaact aaatctagta tacgacttat atcaacacaa gaacccatat gtagtactgg
    22201 tgggattcct actaatttac agacagttgc aagaccttta cctgcatatt tttcagccgc
    22261 atcagattcc attaaaccaa acttagctgc tgctgaagct ccacaacctg tagtaactac
    22321 tattatgtcg tttttgatta attctttcat tattgttacg tgagcctcat ttgaaactcc
    22381 tttagcattg ttacatccaa ctacaccagc tgctcctctt aatactccag cttttagaca
    22441 gtctgttaat ggcttaactg ttcctgctgg gtctatatga gagtttacaa ctctgtctaa
    22501 ttgatttact actgcttcta cactataccc aactgttgcg cctgatttta attcaggtat
    22561 aagtactttt gatttatctc tatttttaaa gtttaaaata gcttctttaa ctattgcttt
    22621 agcatcatca agtgcttgtt cttctctaaa ttcaatataa gttgaacctg ttatcttagc
    22681 ttttggagaa gttgttacaa atttagtatg gtaacaatct gcaactctag ctaatgcagg
    22741 gaatatacat tgaacgtcaa ctattacagc ttcaactgct ccagttatta ttgcaagttc
    22801 ttgttgatgg aagtctcctg ctatttttac accatgtctc attgtaatct cattacctgt
    22861 acaacacata cctgctaagt ttattccatc agcaccaact tctttagcta aagctactaa
    22921 gtctggctct tctgatgcta aaactatcat ttctgataat gctggctcat gtccatgtaa
    22981 tattatgtta actttatttt cttctaatac tgctaaatta gcctctgttc ttcttggcac
    23041 tggagttcca aataagatat cgcttagacg agtacctatc atagaaccac cccaaccatc
    23101 agccatagaa gttctaagac tcatgtgtat taaactatct atatcaccag tacatcctat
    23161 atgagtagag tgcattattg ttgcaatttc tctatctata gctcttggct ctatagcgta
    23221 ttctttccat attttttgtc ttggttctgg tgcatttttt ataaatctta atgtcccaaa
    23281 aggttttcca aattccatta atgcaatttc tgccacttca tgtgctacat catagatatc
    23341 tctaccttct gtctctacat cccattcttt agctaaagta attaacttag cttcgtctct
    23401 tattttatag tttccatttg gatcagctaa tgctaatgta tgtgctatat ctctagcatg
    23461 gtctgaatgg gcagctgtcc caccagcaac cattcttgca aagtttctac ctactatagt
    23521 atgctcatct gctccacata acccccttgg agtcttaggg cttattctac atggtcccat
    23581 tccacatatt ctacagcaaa caccttgaag accaaaacca cattgtgcct taaagccttc
    23641 ttttctatca tacattgttt ctacgccttc ttctctagct ttagctatca tcttctggct
    23701 gtttaaatca attgttaaca ttttttcatc catattccta tccccttttt ttgattttta
    23761 aaatccccca ggtgactgac ttcatgtcat aaaataataa aataaatgct ttctaagttg
    23821 attatatact aattttcagt gctttgcttt agatttaaat atttttttaa caaaattaga
    23881 taatacataa aaattttatt ttctacactt aatttaagtt gattttatcg cttgtttttg
    23941 gctatttgtt aatatctaaa cctacttttt tgatacttca gtttaattaa attatgtttt
    24001 gattttttta aatcaaattt gaaatttttc tagtcatttt tatattattg cagattgttt
    24061 gtgtatttat tatctttcac tactgtttgg acttattaca gatttttact ccctaaactt
    24121 aattagtaaa aacttcttag aatagcattt tttggactaa tttttttgaa tttttttatt
    24181 tttcctactt taatattaat tatagcttca aattgaacat ttgtatatat tcatttgaag
    24241 ctataattat tttttatata aaactttttt aatacagcat taatttagac attttgacta
    24301 ttttttctac tttaattcat ttatttcaag cgatttatcc tatttcatat ttttagctac
    24361 aatagcccca tatgcaatag aatataattt agattcatgt gaatcagctc ttgacactaa
    24421 aactatagga gcttttgcac ccataattat acctgctgat ttagagtctg ccatatatgt
    24481 aaaggctttc cctattccat tgccaacctc tattgttgga accaatatga tatctacatc
    24541 tccagatact tcacttttaa acccttttat ctcactagct tcttttgaaa cagctaaatc
    24601 aaaagccaga ggaccttcta caattacatt ttcaccaaat tctccttctt tacaagcttt
    24661 tgctaacata tctgcatcta cagttgcttg catcttagga tttactttct ctttagctgc
    24721 caaacatgca acttttacag tttccattcc taaagctttt gcagctttta tagaattttt
    24781 taatatcttt gccttttgtt cataatcagg agctatattc atacctccat ctgtagttat
    24841 aagtaattta tggtattttt ctaactcata taccataaca tgacttaata aactatcagt
    24901 tctaagacca tagtctttat ttaacacttc ttttaatatg acagatgtat ctaataatcc
    24961 tttcattaca aagtcggctt ctttacttga tactaattta actgctattt ctgcacactc
    25021 tttgatatca tccttatcca ctattttgat accacttaaa tcaaatccta tttcttttga
    25081 tatttctctt attttgcctt catttccaac caatatagga gtaattatat ttctttctac
    25141 agcatccttt attgccatta aaacttcttt atcatgagct gctgctacag agagtacaat
    25201 tttctctcct cccttaagtt gttctaaaat atcatctaat ttttttatca aaagtaaccc
    25261 ctcccttata tatatttaac taaataatac catatttgct ataccttttc tcaatttatt
    25321 ttttatgtta taaagaattt atatgactaa aagacagttt aatattttat tctgggattt
    25381 catcaatagc tcttaaaagc tttgcaggaa caccacctac aatagtattt tttgttacaa
    25441 ctgctccagc agcgataatt gcattatctc caatagtaac tcctggaact cttcatacaa
    25501 aaaagaggct tatctctatt ttgagatagc cccacttaac cgataaaatt aaatacaacc
    25561 taacaatatc cttacttatt tattccaagt tctcttagac aagcaatagt aatttcatct
    25621 tgtggattta attcataagc tctttctata tagtaagttg catcatcaat atcaccatta
    25681 tttaaataag ccattcctaa attgcaaagt atttctgggt cttcctttat cttagccgct
    25741 tgttcaaaat actctattgc tttatctaaa ttattcttat aagcctcaca aagccccaat
    25801 tccactatag tatccacctg attaggcttt attatcaata tcttctcaag atacatctta
    25861 gcctgctcta tttcgcccat acccttatat ccaagtgcta tcataaacag caagttccac
    25921 caatccatat gttcttcttc taatggaagt aacttctcta atccttcctt aaacttacct
    25981 tggaatacaa gtgtatatcc ttcttcatat tgaactttaa agtccatctt gcctagattc
    26041 tcttgtacct cagctactaa atcagcatct aaccctagcc ttaatgcttc ttcccaagtc
    26101 agcttagtct ttaaatattg accttgatta taataatggt agcctaagtg ataataacct
    26161 aatgcaaaat tctcatcaac atcaattacc ttttcaagct tagccatggc ttcaagtaag
    26221 aatgcattca ttgccttatc atccatatct ttttgatact gtttagctat ctcttggcaa
    26281 acaatcgcat agttatacat tgcatctaaa tcatctggat acattgttat aaatgctttt
    26341 atatatatta aagaatctgt atgttcacca atctcaaagt attttctcga catatatccc
    26401 atatacgatt tcaaatccaa atcaatatct tttgataaag catttaaaaa cttctcatac
    26461 tctgcattgt ttttaaactt actatctatt ccaattatat atatcattgc atctgctata
    26521 gacattgaat ttaacttatc ttgagcagtc ttttctttta tcccttttac taatacctca
    26581 ttttttatag gtacatctaa tccgcctttt ggcactttat agctttttaa tttaaactct
    26641 ccaccatgtt ttatagttat aaaagctaat tcttctgctt tttttagcaa atatttttct
    26701 atcttaaaat ccattttatc ctcctaaata cttaaatctc tactttatga gttttagatt
    26761 tctcaaatat ttttttatca tcatactcaa ataagactac attttctact tctcttattt
    26821 ctgtatttat atcatttttt aaatccacaa cattaaatct aaatggctca aaatgaacat
    26881 attttattat atgtttagct ggtttatcaa caaaactagg taaatatgtt gataagttat
    26941 cattatcttg taaaaataca tggcatctat tcttaaaatc atccacatct agcttattaa
    27001 aaaactgtgg tatatttgat gttttaccca gtgatatata gtcatatttt aatatatcat
    27061 tgaatacatc attatctatg ttgatttttt cattatagaa atccatcaat attttatata
    27121 actgattctt tccttgtgcc aaatcaaagt agccattttc atcaaaatac ttggcaaaaa
    27181 cctcaaagaa tttaaaagga ctttgtttat aaaatctgcc tataatatat ctcatagata
    27241 acacaaagtt tttagaattg tagtatctct ccagtatatc ttctatatcc tttagcttca
    27301 aagtttcttc atagcttata taattattgt ataaaaactc atatggagca taatccttat
    27361 atttaaaacc atgctcatct gcaacttttc tcatacctgt ccctttaatc atctttaaaa
    27421 agcctaattg cagatgctct atacctagat taaatacatc attaaaagaa ttttcaaagc
    27481 tcttataatc ttcataaggc agtcctgcaa ttaagtctaa gtgctgatgt atatttctat
    27541 aagatgctac agtttgtaca acatgagaca actttttaaa atcatccctt ctacctactg
    27601 catcaagtgt tttttcattc gttgactgaa cccctatttc aaattgaaaa agaccttctt
    27661 tacaatcagc caaaaactca agcatcttat catctatcaa atacgctgta acttcaaaat
    27721 gataagttgt atatccatta tcatttttca taaggaaatc catgatttcc atagccactt
    27781 ttctatttgc attgaatgtc ctatctataa actttatctg tgatactctt gcatctatta
    27841 aagcttttaa atcactcttt atcctgtcta aactaaaata ccttagacct ggaatagtag
    27901 atgataaaca gtactggcag ttaaaaggac aacctcttga tgattcataa tacactattc
    27961 tattttcata ttcttttgga ttcaagtttt cataaggact aggaatctca tccaaatctt
    28021 gaagtaattc cataggctta ttcactatta tctcattgcc ttgtctatat acaagtccat
    28081 ttacatcatt tattttcatc tttccttgca agtgaagtac taagtctcta aaaattaatt
    28141 ctccctctcc ataaagtata taatccacaa aatcatactt tttcattgcc tcataactat
    28201 cataactcac ttccggtccg cccaaagcta ttttagtatt aggtctaatt ttttttagat
    28261 tatcacaaag tttaactata tctcccacat tccagatata tgttgagaat aatattatat
    28321 catattcatt tttatatata tcctttaaaa tatagtccaa atcgttgttt atcgtgtatt
    28381 ctttcatatt aacttctatc aaatctctta caaattcttt tagatatctt atagccaaat
    28441 ttgtatgaat aaacttggaa tttaatgttg ttaataaaat tttcaaatat ttctcacctc
    28501 tttttaagat tatactgtct ttttgtaccc cttataggtt aaaatagtat atgtaaagtc
    28561 taaataatat ctatataatt aatttattaa tttcaagtaa tgtgaggaaa ggaagttttt
    28621 tatggaatta attgaaaaag tatctatcaa ctctttatca aaaagagatt tactacttat
    28681 tataaagtct cttgaataca ctaatgaaaa tactcaaatt agtgatttca ttgaccttag
    28741 gaataatata gtaaaggaat tatgtttttt aactgacact aaggaaaaag attttttaga
    28801 ttatctagaa aaaaatagca cactctaata acctaaacct ttttaaactt taatatataa
    28861 atacctgatt tagaatttat ttttatgctt aatatattat cattaaaggt tttcgacata
    28921 actgttattt gattaaatat agggtctatt atagtattac cttcttcaag tcttataaga
    28981 cttcttgggt ctatgctttt attatatgcc ttaactccaa ttttatattt tctcaattta
    29041 acaacatatt cggacataga aaaaataaaa tctatattat cttcatttaa tttactatct
    29101 tcacccaaaa tggataatat agattttttt atgctattaa ttccttcatt ttctacagtt
    29161 ctatcttcca tatatacatc atatagaaca tttaaaacat ttaataaaat actttcttca
    29221 tcataatctt cagatttcaa atcaatgtac ttatcaatta attgtattgc tttaatcctt
    29281 gccatctgaa actttacaac atctgatttg tctaattcta tagaaaatcc tattatcttg
    29341 tctttaatac tctgtagtaa ttcgacatac tctattttac tattatttat aagattgtgc
    29401 ataatggaat tatacactac agaacctaat aaataatcaa ataaaagttc ttctttttta
    29461 aaataaataa catacttttt tattagttta ataacctcat cttcgctttt ttgaatatta
    29521 gtatttgata aaattagtgg tagtaaaata tttaaaaaat tgatatttcc tattttttct
    29581 ccataaaatc tatagtaaat aagttcatct ctcagtctat tttcgtctat atacacacat
    29641 ctttgcgttt tatcatctat taaggatttt gtatttatta tagttaaatc tttaagagcc
    29701 ctacttgcac tactaaaaga agatgtgtag acttttttta gtaattcgtc attcataaat
    29761 atatcctttc tgaaaaattg aatgtaaata aattgttatt aaaaaaaaag agacagcccg
    29821 tttaagaagc tgtcatatat agggggatag tagtgtacaa aaatcattgt aatctactat
    29881 ctatatatta ttatacgata ctactttttt atatgtaaag catcagtttt cgacatccct
    29941 atattttaca gttatacaaa ttgcgtacta agttcaattt tattcttttt atttcaacat
    30001 tttatcatgc tacattcttt ccggagcgtg cattcccaac aatttcagag aattttctaa
    30061 agtttgtttt gttgctaaaa ctaatgcaag tcttgctttt cttatttcca aattttctac
    30121 taaaattgga ttgtcatgat aaaatttatt aaacgcttga gctaaatcta acataaatct
    30181 agtcactata tgtggctcat ttttctttag agcaagtaaa atattcttat tgaatgattc
    30241 tataactcta agaacttctg cactatcttc atcagctaat agactataat ctatatcaga
    30301 agtcacttct acttctgctt ttcttaaaac tgcacagcat ctagcatgtg tatactgaac
    30361 atatggtcca gtctcaccat caaaactaag cgttctttcc caagagaagg tatagtcttt
    30421 aattctactg ttagataact cttggaatac tactgcccca acacctactt gcttagatat
    30481 ttcatccaca tttttagcat ttggattttt agctagtata atttccttag ttttatctat
    30541 agcttgattt aaagcatctt ctaaaaatac tactcttcct tttctagtcg acatagtacc
    30601 ttcttcaagt gcaaccatac caaatccaac atgaattaaa tcttttgccc aatcatatcc
    30661 cataagctca attaccttaa accattgttg gaaatgtaag ttttgttgag aaccaactac
    30721 atatatacac ttgtcaaaat cataagtctc ttttctatat attgcagcag ctaaatctct
    30781 tgtcatatat aacgttgaac catcattctt agtaataagt gctggtggca ttttatactc
    30841 ttctaaatca acaattcttg ctcctttaga ctcttttaaa agatttttct cttctaatag
    30901 ttcgataact ctgttcattt tatcagaata aaaactctct cctgctaaag aatcaaattc
    30961 tatatctaaa agtttataca ctctgttaaa ctcttttaaa ctctcatttc taaaccattg
    31021 ccataattct tgtgcttctt cgtcaccatt ttctaattta ttaaaccatg ctcttgcttc
    31081 atcttccatt tctgggtgtt gttcagcttc atcatggaat cttacgtata attttagtaa
    31141 ttctggaatt ggattagact ctactacttc tttttctccc cactttttaa aggccactat
    31201 taacttacca aactgagttc catagtcacc taaatgattt attcttatag tcttgtatcc
    31261 ttgcgaatca tatatcttat ataaagcatt acctataact gtagttctta tgtgccctat
    31321 atggaaaggt tttgctatat ttggagaaga atattcaact attacagttt tatcttttcc
    31381 aaaatcacta tgtccgtaat tttctttttc atctaaaact tttttaatta cagtttctgc
    31441 taattgagat ttatttacaa agaaatttac atatccgcct aattgtatta cttttgttat
    31501 ctctccactt ggttctatac tttccgccaa ttcacttgct atcatgtttg gagcttttct
    31561 aaacactttg gctaatttaa agcatggaaa agcatagtcc cccatatcct tatttggtgg
    31621 aacttctata agtgcctcta tctcttcttt cgataaatct tctatttttt ctttaaggca
    31681 atttgatatt gccaccttaa aatcttgcat tctgttgctc ctctctacat tgttcctggt
    31741 ttatattgtg cttatctctt tatgttaaat caaatttcgt gttttttcaa gtatttcatt
    31801 acagtgtaaa acactttaat aattatgttt tctgtatata ttaaaaatac tataattgca
    31861 gtttttaatc acactattat tgatttttta taagactttt ttctatttta ttgtttcttg
    31921 tactatccag ttgactttat atactttttt taatctttta tcaattttca tacatcaata
    31981 ttaattttat atacatttat tttgctcttt tatctatttt tatacatcaa tattagcttt
    32041 atatactttt tttactcttt tatctatttt tatacatcaa tattagcttt atatactttt
    32101 tttactcttt tatctatttt ctataaatct ttttcactct taatttttat tccttcttct
    32161 tctaacagcc ttgctgttat acctttccct ggtatctttt tacctgaaaa actaccatca
    32221 tatattagat tacatccaca agaggggcta ccctctttta gtattgcttc gctacataca
    32281 tataaatttg ctatttttag agtttcatta gcccctctta caaagttttc tgttacatcc
    32341 ttattatctt tagtaattac ttttccatca actatctcag caggtggtct tggtgtagat
    32401 agaccaccta actgctctgg gcatacaagt gtaaattctt tgccttttaa atattccttg
    32461 actttttcat tttcattatt atcgccacta tatttacaat ttattcctat taaacatgca
    32521 gataccaata tcataaaaat gtgctcctat acaaattctt aatatattct tatacaaatt
    32581 actaatgtat tcttatacaa attattttaa tttaacaatt gttactcctg ctccaccttc
    32641 tccatattga ccacctcgtt gtgattttac atgtctattt cttcttaaaa tgtcttgtag
    32701 ccctgctttt aatactcctg tgcctattcc atgtattact gttacagact ctaaacctgc
    32761 cacatatgca tcatcaagat acttttctac ttccattata gcttcttcta ggtttaaccc
    32821 tctaaggtca acttcatttt ttacactacc agattttgac cttataatgt ttctcgttga
    32881 tttagttaca tttgttgaaa catctttgcg tgttttttgt aaagatttaa aaggtagagt
    32941 catcttcatt ataccaattt gcactactgc ttcttttctc tttttatcta ctgaaactac
    33001 actaccattt tgatttaaag ttattacctt aacttcttca cctggcttta agtcttttat
    33061 ctccttattt gatacctttg gaactatcat actttttact gttggctgaa gacttcccat
    33121 agagctggtc aattcttttc taagttcttc tatcttacga ttcttttctt ttgaagctct
    33181 ttcttgttca agatttctaa gttcttttat tataatatct acttcttctt tagcttgtct
    33241 tataattgaa aatgcttctg atttagcttt ttcaatcatc ttatctcttt gactgaccaa
    33301 tttttcaagt ttttcgtcat actctacttt cagtttttca atctcttctt ttaatctttc
    33361 agcttcttct ctatcttcta ctgctttgat tctattcttt tcaacattct gtaacacatc
    33421 ttctaaagct atgttttctg tatttatata ttctttagca cgtgaaatca cataatcact
    33481 tagcccaagt tttctagaaa tctcaaacgc atttgatttt ccgggaacac ctataagtaa
    33541 cttatatgtt gggcttaaag tttctatatc aaattcaact gctgcatttt ctacacctgg
    33601 tttagtaaga gcataatttt taagctcact atagtgagtg gtagctatac atttagctcc
    33661 tacagaattt atatcttcta atactgctat tgcaagggca gctccttcaa cagggtctgt
    33721 tcctgctcct aactcgtcaa atataactaa agagtctgct gttacgtttt gtaatataga
    33781 tactatattc gtcatatgag atgaaaatgt tgatagactc tgttcaatac tctgttcatc
    33841 tcctatatct gcaaatacat tatcatatac acacatacta cttccataat cagcaggtat
    33901 atgaagtcca ctttgtgtca taagtgcaaa tagacctaca gtttttattg taacagtctt
    33961 accacctgtg tttggacctg ttatcaccaa tgtatggaag tctcttccta aataaattgt
    34021 atttgctact accttctttt tatccagcag tggatgtctt ccatttttta tgtttaaata
    34081 tttatcctca tttaaagttg gttctattcc tctcatctgt atagaaagtt ttccctttga
    34141 aaatgcaaag tccaatcttc caagtatctc ttgattagag attaaatctt cgctcacttc
    34201 tccaaccata gctgaaatct ctgataaaat tctttctatt tcctcttgct ctcctagttt
    34261 caactgcctc aattcattgt tcatctctac tatagtcatt ggctctataa aaagtgtcgc
    34321 accagaagat gactggtcat gcactatacc agctacttgc gacctatatt cagattttac
    34381 aggtactaca aatctatcac ctcttagaga aattattgca tcttgtaagt acttttgata
    34441 agtagttgaa cttattattg aatttaattt tgacctaatg gattgatttt tttgggctat
    34501 tcttcttctg atatctctaa gtgtacttga agcattgtca gatatttcta cttcactaat
    34561 tatagcattg tatatctggt cttctatgtc cttgtacaca tatagagaat ttgatagaga
    34621 ttgtattatt ggataattaa aatcttcttc atcactacta gataagctat ttcttaatct
    34681 tctagccact cttaaggtat ctgctatcat aatcagactt cctgggtcaa gagaagcacc
    34741 tatataagct ctctttacct tatcttctat atcatgaata ccttccaacc ctactgaacc
    34801 tctctttata ataattgatt gagcttcact agtttcttct agcatagatt tcacttctac
    34861 aaaatcagta tttggtacta aattttctat atattttaga cctaaagaag atgaggcttt
    34921 cttctttaac aaatctatta ttttattgta ttctagaact cttaacgatt tctcattcat
    34981 tttctatatt caccatcctc gatattttaa cttttttatt taattttaaa aatgtatgtt
    35041 ttcaagcatt tccaataaaa aatgcaagtt ttatttcttt ttcatttcta aaattattat
    35101 attttttctc ttttagatat attttacaca tattttgtat tatactatgt atgtagctat
    35161 ctatatatta tacataaaca tgctttattt gtaatataat ctgttaaaaa atcttcttta
    35221 atatttttaa aggagtttat tttagcttat aaaaaaatag ctacttatat agataaatta
    35281 aggaggaaca tgagtcatgg gaaacgtatt agaaggatta aaaccagaaa gtgtttttaa
    35341 aaactttgag aaaatatctc aaattcctag aggttctgga aatgaaaaag gcataagtga
    35401 ttttttactt agctttggta aaaatttagg tcttgaaact attcaggatg aaagcttaaa
    35461 cataataata agaaagcctg ctacaaaagg atatgaaaat tgtccaggtg tagttcttca
    35521 aggacatatg gatatggttt gcgaaaaaga aaagaatgtt gaacatgatt ttcttaaaga
    35581 ccctataaaa ttaagaattg atggtgacat gatatatgct actggaacta ctctaggagc
    35641 tgacaatggt atagctgttg ctatgggtat ggctatatta gaagataata ctttagagca
    35701 tcctgcacta gaagttttag ttactgttaa tgaagaagat ggtatgaatg gtgcagatgc
    35761 actagaccct agcttaatta aaggtcaata tatcctaaat atggactccg aggaggaagg
    35821 atacttatta gttagctgtg ctggtggtaa aacttgtgtt gttagtttac ctgttgaata
    35881 caaggaagtt aaaggtgaca aacaaggatt attagttgag gttactggac tattaggtgg
    35941 acactctggt atggaaattg ttttacaaag agcaaatgct aacaaagcaa tagctagagt
    36001 attaagtgta ttgaatgttg attatgaatt agcttctgtt gatggtggta ctaagcacaa
    36061 tgctatacct cgtgaagcta aatgtgtaat agctgttaat aaagctgatg ttgaatctgc
    36121 taaaaaacaa ataaatgaca ttttaactgc atttaaacat gagtttacta cttctgaccc
    36181 tggcatgact tactcagtag ctgaaacttc tgtagacaaa gttcttacta aagattgtaa
    36241 agaaaaagtt gttcaaatgt cttgcttaac tcctcatgga gttcaatcag taagtcttga
    36301 tatagaagga ttagttgaaa gttctactaa cttcgctata attgaaacta aagaatctac
    36361 tattgaattt ttaacttctg ttagaagttc tgttatgtct attagagatg aaatcgctga
    36421 tagaataaga cttttggctc aagctttagg tgctaattac gaccttatag ctcaataccc
    36481 agcttgggaa tttaaaaaag gctcaaaatt agaaaaaata tgcagtgaaa cttatgagaa
    36541 attaactggt aaagttccta ctgttatggc tctacatgct ggtcttgagt gtggattatt
    36601 acttgataaa cttcctcatg ctgaagctat ctctatagga ccagatatgt ttgatgttca
    36661 tactccaaat gaacatgtta gtatcccttc tgtagctaat gtttgggatt atgttattga
    36721 aatattaaaa tctatgaacc aatattaaat ttaagttttg attctaaata gattttaatt
    36781 ttaagtaatc tatataaaat taactctgta aattaatact actgttagta taatatttac
    36841 tttcaatatt aacttataga aataaagcct aatgatagat aataacatgt atcattaggc
    36901 tttattgtaa tttaaatatt attaaacttt attctgtttt aaaattaatt cttatactat
    36961 tttaaatcta tttcttcctt gtctcttaga atcataaaga gcattatctg ctatttgata
    37021 cagttcatca tactcttgac cacgctttgt tataactacc cccatactaa tagataaact
    37081 ttctccattt ggcaacttaa tgagatgaat atgattgtat acatcattac atttattgat
    37141 aagttcttcc ttatttatta tatcatttat ataaacacta aattcatctc cacctaaacg
    37201 acctactata tcatcttttg caaaactttc atcaagtacc tttgcaacat caatcagtac
    37261 tttatctcct accaggtgac cataagtatc attaattgac ttaaaataat caatatctat
    37321 aatcaataaa gctccatttt tttcagttga taaatttaat aaattttgtg tagttaattt
    37381 tctaatatat gctgcattat aaatatttgt taaactatcc ctttgaactt tatccaacaa
    37441 tctattcttt tcctcttttt cagaatcaat attagctatt ttaccaatta catacattgg
    37501 tatttttcta tcattataaa ttatttttga agtaattcta atccaatgaa aagtatcatc
    37561 aagcatgata gaatacattt cagtagtccc attttcttgt gattttatta catcaaaaaa
    37621 ctttttatta tattctcttc ttattttatc tctttcattt tctatattgc cattttcact
    37681 ttccttatca cttttatcca aaccataata agtctcaata cccttttctg gcatagaaat
    37741 tatcaattta tcttgcttat aatcatactc aaaaaagtat tcacttgtca tttcatacaa
    37801 ctgagtatgt tttttgagtt ccaaagatat cttttcgttc atacttgtac gagtatgtaa
    37861 accaaatgcc ataaatccaa taatacaaat taataaactt gctacaataa aaatagtctc
    37921 aattggattc tgttgcataa tataaagcaa cgagaaatct tgtctatatg tagtattttg
    37981 gtagacaatt gattgcattt tttctgatga aatacttata atagctttat taaatatatt
    38041 caataattct tgtttatctt ttttaattat gccaaaacaa atactctttg cttcataagt
    38101 ttgaggaact aggcgcacat ttttatactt aggttgattc atatagtact gcatagaata
    38161 accttctcca taagtgtaat ctgcatagcc tttattaaca gcttctatac actcttctaa
    38221 attatcatac caaactatat tttcttcaaa ctgaccataa taatcaatac ctttggctag
    38281 agctatacgc tttccattta gactattctc atttaattta ttattaatca tcatagtata
    38341 ttgagtagaa agaaaaggcc gagacatagc tatattatgt tcacgagagt actcataatc
    38401 ataaatcatt cctgctacaa tatcaatttc atgtttttta gccatatctt ccaaatcttc
    38461 ttgagatttt acaggcacaa gttcaaattc caaacctgtt ttatcacata tatgatttaa
    38521 caaatcaatt gatatacctc ttaattctcc tgtatcttta tctgcatttt gaaacggtgg
    38581 tttgtttaaa agtactccta cttttaattt ttctgtatct ttaatgtaat tagtttcttc
    38641 atcagataaa aacatttgat tattctctgg cgtaaagtat ttctcatata aagtagttga
    38701 aaaatatggg tccttctgtt caatattatt aattgctgaa ttaagctttt gagttaagtc
    38761 tgtactgcct tttggagtaa tgaaataaaa tggctttgat gagaattttg ctacagtacg
    38821 cacaccttct attgggttca tactaacatt aagaagtaca tctgctttac cactctttag
    38881 tacttctaat tgctcgtcac ttgttttaca atataccaat tcaggtgaca taagattcat
    38941 tttacaatac tcatctaatt ctttaagcct agtatcagaa ttttctatta cagcaatacg
    39001 aatttttttc acatcttgcg aactcattat gttatcaata tctgtatttt catataatgc
    39061 ttgtagtact gtatttgcta ctccataact gtttcccgca taattatata ttttttgcat
    39121 tggctcattg tataacatac ctcccattat atcaatttca ccattttcta acatcttcat
    39181 taattttgta atagattcat ttatctctcc aggaacttga acaaattcat aattccatcc
    39241 agtatactgt gcaatttcta gcaaatattc atatgtatac ccaaaatagt taccttgctc
    39301 atctatttca gttaaacctt tttgaatagg ataagcaaca cgaatggtac gattatcatt
    39361 acctatagca ttgataccaa aagaaaatat gcaagaagtg aatatagatg taattacaat
    39421 aattaataaa agtaattttt ttaaattcac tacacaatcc tccatatagt taagatttac
    39481 agtataaatt actgcataaa cttgtagaca tgaaaaaata gattagtata taaaaggata
    39541 tataaatcta cttagaaatt taaatatcct taatatatac aatatattta aattataact
    39601 catttatata tctattacaa caaatttacc ctgctaaata cctactctat atcccataat
    39661 tttacaagtt gtaatgaagt ttaagttcta tctcatttca aatttagcag tgtttctatt
    39721 tacagttctt ttatatttca catctggata acccaatata aatgaagtga taactgcttc
    39781 gttctcgctc attcctaaca gttcttttat ctttggctct acatttgatg ccataacaaa
    39841 aaatccatta taacaaattc ctaatccttg agaataggcc ataagttcca tattagatgc
    39901 agccaatcct ccatctacat aagcacttcc acctagagac atatcaccta caactatcat
    39961 aagtagtggg gcatcataaa ataaagaatc atttccgttc tctttatatc ttttgtacat
    40021 tttcttaaat gtatttttat agatagaacg tacagggtca ttgtcatcag tatctaatgc
    40081 caaattataa agaccttgta ttgccagttc tttcacttct tttagctttt cttttactaa
    40141 tatgtatctt ataggctgtc tattaccacc tgttggtgtg tatctaccag cttccactat
    40201 gttttttatt ttttcttctt ctacctctat atctttatat tgtcttatac ttcttctaaa
    40261 tttaataaaa ttcaataact tttcgggttc taattcaaaa ctttcacgat tgtattctat
    40321 tacttcattt ttatcaaatc cttgcatttc tattgcatct gttgggcaaa ttgccataca
    40381 gtgaccacag ttaatacaga gaatattttt aacctttgct ttatcatcct taacttttat
    40441 tgcatttaca agacagtctg attcacacat cccacagcct acacattttt ctaaatttat
    40501 gtctatcatt ccaaattatt cctcctttta aaaaatttac tttagtataa aacaaatagt
    40561 tgtctaagtc aatttttatt tttttaacaa tatactagcc ttaactacac tttaatggag
    40621 aaaaatatac ataaaattag aacttaaatt agaatttatt caaaataaaa gataaaagaa
    40681 gtaaatgtct aaaagtgaac tttttagtta atgagacatt ctttattgta tgaaatcaag
    40741 tggatattta tcatttcaac aataaaaaag gcttacctct attttggata tgtccttgtt
    40801 aagtagacag actaaaaaag gctatcttta tgcactgaga tagccccttt tcccaactac
    40861 tattgtttat tttaatatta tatcaataaa tttaatttgc tataacctgt aagttacatt
    40921 ttaatttgat gtaactttaa attacatttt taagtaatat ctttaaatta tattcttaaa
    40981 atatatcatt aaactacatt ttaattaatc taaaaactat atcttaatta atctaagaaa
    41041 cttacattat aatcttttag tttcccatta tcatatacaa tctttgcact aaagatattt
    41101 ccagtcttag cccaatttaa gaacaacctg tcaccttccc acatatttaa atcgaatatt
    41161 ttatcattac caacccaaat taaatttcct tctttacaat catttaaatc tttttcagac
    41221 aattcacctt caaactcatc tgagaaatac aaaaacatat accaatcttc ttcatcttta
    41281 aattttggaa aagttatcaa ccctttatat gacaatgatt taagagtaag accagtctct
    41341 tcctttactt ccctaattac acattcttct ggagtctctc cttgctcaaa ctttcctcca
    41401 actcctacat acttaccttc atgtatatca tctttctttt tatttctgta caacatcaaa
    41461 gtcttgtcat ctttttctat ataacaaata gttgtaagtt tcatgtatct cgccttactt
    41521 tctctttata ttctcaatta attattttca gcactttcaa gcacatcact tatattcttc
    41581 attgcattaa gagaaagttc tgcaaattca tcaaatggaa tccctatatc ttctatactt
    41641 ctcattgcat ctctatttac atttgaagca aatctcgttt ctttcattct tttaataaca
    41701 gacttagtct ttacactagt aagtttttta tctggataaa ctaaagctat agcagttata
    41761 aacccagtta tagggtctgc tgcatatatt gctttttcca ttaagctagt tcttgtaact
    41821 cccattccat catgatgacc aagtattgct ctatacattt cttcgtcacc gaagttttct
    41881 tttttaagca gttctactgt tgttgtagca tgtccttcac atatttctcc tggctttctt
    41941 ccaactatat ctgaatccaa atcatgtaaa agacctgcca ctgcccactc ttcttctttt
    42001 tctggctcta gtcttttggc taattctctc ataacagctt caacagcata acaatgtttt
    42061 ctcattctat cagtttctaa atacttaaat aaaatttcta aagcttcttc tctcattttc
    42121 accaatcccc ctgttttttc acatatatga tatttttata tattaataca cataaattca
    42181 agtctttata tatattcatg tatatttttc atgttatatc tattctacac ctttatagta
    42241 gtgtcctgca actgttccaa tttcatctaa tttcttatac aaatctgtag ccttttttcc
    42301 aagtttataa ttattactca atacctcaat gtgagcttca ataacctttt taaccatctc
    42361 actatcttca actgtaaaat ctatcctaaa tacatctatt ccaatttcct ctaactcata
    42421 tagattgtcc aacatacaag taaccttcga attgtaaatt gtacttctac agaatatatc
    42481 ttgtgttatc ctataacttg catccgtaaa gtctcttaaa gcataactac tttccttaca
    42541 tttagcaact ctcttgtcct ttttacaatc tctaacaact actcccatag gacagtattc
    42601 tgtaaccatc ataggagcat atccatatat tactgcttca atttctacat ctgtatattt
    42661 taaagtttgt ttaatttcat ttaagtttag ttcttgtgac aaacaaactg tacttgctcc
    42721 ctcttctttg aagtggttta tactttcact attgaatgca tttaagtagc aatctatatt
    42781 taagtctttt ttcttaaagt attccataga gccccagtta ccaatttgaa ccttattaat
    42841 tccttttata ttatccaata tctcaaattg cttgtactct ttatttctaa caatccttgg
    42901 tgctgaatag attaattttt tgtcagtgac tgctgtatta tagtggttct tattcaaagt
    42961 atctatttta cttgtatttg acaaagtatc tatgttattt gtatttgaac ccattagttc
    43021 tttaggaata cctaaagacg aagaagtatc taaatccata gaaatacttg aatctataga
    43081 aatacttgaa tctatagaaa tacttgaatc tatagaaata cttgaggcca tttgtaaagc
    43141 ttttgataat gtatttatat cttcataata gattaaatct acattataat ttaatactgc
    43201 ttctaattgc tccaagttct taactttaac tctaatctta ggaactgtat caagtttttt
    43261 ctgtttattt actctatttg gttggtattt taatctattc ttcttaaatt ttctatcttt
    43321 tatctttatt ctttcttcat ctaaaagctc aatacaatcc cttctcattt gatttaacac
    43381 acttataggc atacttacac cttcatccag gtcaatttct atctctttta acttatatgg
    43441 tgtatttcca agcttttgaa tttgtgtttc tattttttct ttacttaaag ccacctttat
    43501 agctttttca actattttgt ctccttctac caccgattta tttccacaaa aatcacttat
    43561 aaaaagctct ggctttttac ctagtttaat actcactttt gcacgtatat tagtttttat
    43621 gaactcttta tcttgctgaa atgtagcttg tactctattc attaattcgc tgtcagaagt
    43681 cttatatata agctgatttc tctttgcttc tccaacaaaa tctaattcta tcaaatcccc
    43741 tttatgtgct attgtagtta tctctccatc ctttattatt cttccaatag tacctccacc
    43801 taagttaatt ccatcacctt tcttaagcgt attttctagc attactctaa gtcttttgtt
    43861 ctttctatta taatctaaaa cttttccaat atatagccct tgattattag gtctattaga
    43921 gttcattata tccttaccaa cttcacctaa tatatatcct tttgtaaact ttctattaaa
    43981 tatggtatac aaattattta ttatttcctc agaaatactt atttttttct tatctacaaa
    44041 ttcattaatc gcttctctat atcctgaaac cacagtagct acatactctg gttttttcat
    44101 tctcccttca atttttaaag ataatacacc tgtttcaatt attttatcca aatcttctat
    44161 agtacttaaa tctctaggac tcaacaaata atctccatta ctttttacta cttctcctgt
    44221 atttatatct atcaactcat acttttgtct acatggctgt gcacacctac ctctatttcc
    44281 agacctattg cctatcatac tactcataag gcattgaccc gagtaacata cacataaagc
    44341 tccatgtaca aaaacttcta tatctacagt tgtattttca caaatccatt ttatctcatc
    44401 aacatttaat tctcttgcta aaacaactct atcaaaccct acactctgaa ggtattttac
    44461 atcttctaag gaatgtgcta ccatttgagt gcttgcatgt agttcaaagt caggtaatag
    44521 tttttttata agtcttgcca ttcctatatc ctgtactatt aaagcatcaa tatctatatc
    44581 gtataaaaat tttacatact ctacaaaatc ttctatttca tactgtttta tcaatgtatt
    44641 tacagtaaca aagacttgca ctccccttat atgagagtat ttaacagcct caatcagctc
    44701 atttctatca aagttatttg cagatgctct agcactaaag tctttaccac ctaaatacac
    44761 agcatttgct ccgttttgaa ctgctgcttt taatgaatca aaagagccta ctggtgcaag
    44821 taattctatt tttttcatat ttttctcctc tatttatact tgttaggtta atataattat
    44881 atctataact taaatatttg tattaaattt atatatttct tactatattt taaattcttc
    44941 aattaattta tatattagtt atttatattt ggtccatttg agttaacata
//
LOCUS       DAMJWP010000010        39586 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.010, whole genome shotgun sequence.
ACCESSION   DAMJWP010000010 DAMJWP010000000
VERSION     DAMJWP010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 39586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39586
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.010"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(94..1695)
                     /locus_tag="PC700_000502"
     CDS             complement(94..1695)
                     /locus_tag="PC700_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF2579827.1"
                     /translation="MIYDLILKNGFIIDGTGNPGFYGDIAIKDNLIAKIDSKINSNTN
                     KEIDCCGKVITPGFIDPHVHEEIVAILDGKFEKFLKQGVTTTINGNCGHSITPYSSEN
                     VYEYMYKNGLLLEEEKKYLIDKNKCWNNFTEYCDLISKSGISVNMGFLLGHGTIRWSV
                     MGGSKDRPPTEKEKNEITDIINDGMKSGAFGISTGLAYIPSKYADIDELVDIARQIKE
                     HDGIYTSHIRDYIGRYNAVKEAIEVGQKSGARVQVSHLSPVEIEAFDEILKARYNGVE
                     IMVDTVPRSSGHCMKKKRVIQFIMAISSSLFELGIDGVMDDLRNEEGRTLILKEAFIL
                     GDRGSIILLNTKDINMEKKSIREIATQKGIDEDKLLLDLLLDGDEELIFCLGGMYRAD
                     FPDKLHDNKIIDNPFVMVGSDCLFSVGGDMSWFELQRRGAFPIFFNMYRKSGVRLEEI
                     VRRVTSLPARQFKIKNRGIIKEGLIADIAVIDMDNYSYSRSEDIDFSKPQLLANGVEY
                     VIVNGKIALENGEITENKCGEVLKR"
     gene            complement(1806..2483)
                     /locus_tag="PC700_000503"
     CDS             complement(1806..2483)
                     /locus_tag="PC700_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2579828.1"
                     /translation="MSKVKSIYNEEYLPFMIRYGRLTLSLGIIAALVPGIILSFGFGI
                     MPPISALLASTMAIVSMSAPNYIIEPVSYSPILGIPGTYMSFLSGNISNMRLPCSIAA
                     QKAAEVESGTEEGSIISTIGIAVSILVNISILTIGVILGGSVLSKIPAEVVEKLNLIL
                     PALFGSVFGQVFLQDKKLGLVAIVISVLTIILSKQGIIPQSLVVLICVFGTILIARAM
                     YKDKLSD"
     gene            complement(2496..3191)
                     /locus_tag="PC700_000504"
     CDS             complement(2496..3191)
                     /locus_tag="PC700_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HDF2579829.1"
                     /translation="MDYLKLANHPLLWIASTVAVSIVVVQSLIFAIKSCKVGKTMGIT
                     DKQIKSAVKSSAISAIGPSMTIFAGMVSLIVTMGGPIAWMRLSFIGSVIFESMSAGFG
                     TDALGITLGSPEMTKLAFTNAVWTMILGSLGWIIFTLLFGHKLDKITNVISSGKKSFI
                     PIISLGAMLGSFAYLNADRVLRFDNGTIACISGMAIMVILCMLEKKKNIKWLREWGLT
                     ISMFSGMIIASVI"
     gene            complement(3243..4442)
                     /locus_tag="PC700_000505"
     CDS             complement(3243..4442)
                     /locus_tag="PC700_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HDF2579830.1"
                     /translation="MKSAYKGYEAYSYLEKGKDISSIEMCKGDKRVKEYLIELDDIQE
                     AKVKSIVDEKIFISLHEHPVLFPEHLERDIFEYNREGKQRCAYEALSRGYYDAIFDNL
                     MDGVCTITSNSGWKWDDILIDLGMRLCDLAHQDFVIKCEKVEDICRAKSEGKVALIPT
                     LEGAAPIENELDRIDILYGFGIRLMGITYSESNALGSGLKEEKDGGLTNFGKKAVERM
                     NKIGMAIDCSHVGVQTTLDVIEYSKDPIILSHVGARNLWNTKRLTPDEVLIACAKKGG
                     VIGVEAAPHTTITEKNKEHSIESFMEHFEYIKNLVGIDHVAFGPDTVYGDHVKLHSVL
                     AAKFDIKKITGTKEHPKVEYVKGLENPTETSKNIVRYLVKHGYSDEEIEKVLGGNILR
                     VLKQVWK"
     gene            complement(4703..6748)
                     /locus_tag="PC700_000506"
     CDS             complement(4703..6748)
                     /locus_tag="PC700_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2579831.1"
                     /translation="MKKHNILFVSTDDKINIDISKQLENIFGEFCNIDNLVYVNRINI
                     ELSSYELVVCSDNDIKEYIHNNIDKNILIVIVHRTINIENINQIISIENDSDVMVIDA
                     YKESADETAKIIRKLGLIHINLIPYYPGCDKSKCEIGIITGSRNSIPQNIKQIIDIGD
                     KVIDINTVIEIFTKLNISIDKLHIIKEKYNEDTVSGYRYYTTMNKTMKSFLEIIDEGI
                     ASVDKLGKFIYCNKVFSNLVGIDQNEIISNNFMDLFSDKVVKKIFFQEDEVNDEVVNL
                     NNKKLIINKVNVYENNERIKSIISIKNISAIQVLEDKIQNKLQSKGFVSKYTFESVVG
                     ESKIIKEKINIARKIATTDFSVLILGENGTGKEIFAQAIHNESLRKNKPFVAVNLSSL
                     SDTLIESELFGYDEGSFTGAIKGGKMGIFERAHTGTIFLDEIGDISLDVQQRLLRVLQ
                     EKEVMRMGGSKIIPIDVRIIAATNKDLKKKIKEGSFREDLYYRINVLHIEIPRLRERK
                     EDIPLISKYFLDEINSNKCFTEESMQALTLYEWPGNVRELKNLIYYIDTIVEEDRVDY
                     EHLPEQFRFEKNNTLVNENFDSIILDFKQSNFFEESICILNSIETWNNKNILLGRNKL
                     QEILKEKDIVLSVDQIRKRIDKLKSHGLLLSGVKKQGSFITDEGKNFISYVKIKGVI"
     gene            complement(7159..7968)
                     /locus_tag="PC700_000507"
     CDS             complement(7159..7968)
                     /locus_tag="PC700_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2579832.1"
                     /translation="MYYVYVNDINIAVYDLNPSAKKTVLFIHGWPLGHKIFEYQTNIL
                     PKLGYRTVSIDLRGFGKSDATSGGYTYSQLADDIYKVVHAIGLKDFTFVGFSMGGAIV
                     LRYMSLFNGYGVSKLVLAAAAAPSFVQRPPEFPYGMTREDVNKLIAQACTDRPEMVTD
                     FGEKVFASNPPESFRRWFNDIGFSASGIGTIGTAVSLRDEELFNDLKCVRVPTGIFHG
                     KLDEICPYEFAVFMNEKIEDSILYTFEYSGHAIFYDELKLFNQEFLQFLEE"
     gene            complement(8052..8585)
                     /locus_tag="PC700_000508"
     CDS             complement(8052..8585)
                     /locus_tag="PC700_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDF2579833.1"
                     /translation="MSDAHYDMHKRNGYSSDEPYPEIKVLGLNKYYAELLMDDYAGVS
                     SEFTSVNQYLYHNFDLDETHRELSEMWINISITEMLHMEILAKTIRLLGGNPVYRGST
                     SSCGAYWNGGFVCYGNSICNRLKLDLHLEHVAINNYYKDISLIEDPYIKAILNRIILD
                     EKLHVSLFEKAIEKYCK"
     gene            complement(8712..10787)
                     /locus_tag="PC700_000509"
     CDS             complement(8712..10787)
                     /locus_tag="PC700_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2579834.1"
                     /translation="MLKGLSFRNARRQAGKYLIYWCSLIGSVALIYAFNALIFSDVMQ
                     ELSSILNNGGSDEFSYMIIIFSFIITFILGWFVSYMMNFILKKRSREISTYMILGVDK
                     KQIVKMLFIENLLLGASALIVGWILGIFVSKVLESIVINMFHGKYSLSPDFSLKAAGM
                     TFLYFCIIYLIAQLKSNKKLVKMKLINLLNYDRYNENVLIHQTKTKSIMFCVSVLCGG
                     MGIFFFSIPSGRLTDVFLGLLLIVFCLIVLFMGFGSIAQNMFEKGMNWKYKKSHLFLY
                     RLLASKINGISIIMGIIATLFTVSIACIGIANSFYSVMNKSVDLQQFDVSIFHPNESY
                     DFSSYEGYLSETKGVKKDYSYTLYTEEKTSFMKVRNNILSKYLKDIGRDLQYKDYTFA
                     ENQFDTFMKYSDYNILRNMLGLNPIKMDKNQFIIHCMPYLADAYKNYIGEKYKLTISD
                     KDLSIAGIYIENFSQYGGYGNGQEYIIVIPDYLIDNMKPLYSLYVAITSEPLDSAYFT
                     EFQKKFDTLENLNSGVVVAGNQGFVSKLRYDNKDYISGKYAMQSTGQAIILILPLFYL
                     ALIICIIGTVILSIQLLSENNIIIKHYGMLRTLGMIDSEVIKILKKHVCLYFSIPLIP
                     AVIFGGILVSVFSYNMFNISFDAPVFFNIQIPILHAVAITLINFVVIYCIYVFITYVA
                     LRKEILHIK"
     gene            complement(10790..11536)
                     /locus_tag="PC700_000510"
     CDS             complement(10790..11536)
                     /locus_tag="PC700_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2579835.1"
                     /translation="MLLRVENIEKYYGNQKILTKALNDVSFQINTGEFVSIMGTSGSG
                     KTTLLNCIATIDTVSAGKIYLENQDITELSEENLTVFRRDNLGFVFQDSNLLDTLTLE
                     ENIALPLTIKKVSPVEITNSVYNIATQLKIEEILSKFPYEVSGGQRQRCAVARAVVTE
                     PKLILADEPTGALDSNSSKTLMELLETINTILEATILLVTHDAISASYTERILFLRDG
                     KIFNELRKGEKSRLTFYHEILDALSLMGGD"
     gene            complement(11550..11945)
                     /locus_tag="PC700_000511"
     CDS             complement(11550..11945)
                     /locus_tag="PC700_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HDF2579836.1"
                     /translation="MKKRILTILCVVLVLFISITGLDSMGFFSRSIIPLRSEYASFIR
                     KNGTITEVKFTGPFNHNNIDLNKNVEDTNKNGDDNELTIKYDIEVKKSLISSNEPTIQ
                     IQDSKESIYVYILNFSDKTIIIKNGKIVD"
     gene            complement(12058..13080)
                     /locus_tag="PC700_000512"
     CDS             complement(12058..13080)
                     /locus_tag="PC700_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2579837.1"
                     /translation="MRFCNFLQDKVLVILLNICCAISLVLYLHLIGIGQGELTLLIAA
                     WVAILFCWICFCYQKQKKEIIKLRRIVDELDKKYLFIEMIEKPTSNIEREYYNIIRLS
                     LKSMTDHVSQIHREMYEYRQFIEQWVHEIKHPVTTSELICENYKNEHTRKIRFQMEEI
                     ERHIERALYYARLGHVEKDYLISEITLKSIVEEVLAKNKLLLIQNNIHINIQDLEYSV
                     YTDKKWIVFILNQIIINSVQYKSDSAEISFFAIKEDKSVKFTIKDNGIGIKESEIYRI
                     FELGFTGSNGRTTNNSTGIGLYLCYELSKKMGIDIDVSSSLGEYTAITLNFPINTAIN
                     MQKQCN"
     gene            complement(13077..13748)
                     /locus_tag="PC700_000513"
     CDS             complement(13077..13748)
                     /locus_tag="PC700_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005924879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2579838.1"
                     /translation="MILTNILIIEDDVFICKELMQFFENNGYSTQTLDTNNCDVLQVI
                     QESTPDLILLDINLPNIDGFSLCRQIRNCSTIPIIFITARDSAIDELNGLTMGGDDYI
                     TKPYNLPLLLARVKNLLKRVDSSDKQKIKYREIILNLISATVEYQNAVIELSRTELKI
                     MYYLFQNVGSIVPRIDLVEYLWDNHIHIDDNALSVNITRLREKLRGIGITDLIITKRG
                     MGYRI"
     gene            complement(13834..15183)
                     /locus_tag="PC700_000514"
     CDS             complement(13834..15183)
                     /locus_tag="PC700_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HDF2579839.1"
                     /translation="MELKNFKDILFKKALSEGFEECEIYYYTGENLSINIYEGEVEKY
                     NLDKSFGLSFRGKVNGKIGYSYTEILDDKAVDMLIKNVKDGVNTIENEDVQFIYEGDK
                     HYNDVKTYSKELENLEADKLIDLALEMERETKAYSDKVVNLSKCTISYSVSSNGISNT
                     KGLNLSNKTNMLIGFVVPIIEDNGQKYDGIGYNRANSIEEIKPCEISKLGVDNALSKV
                     GGKSIPSGKYKTILLNEAMVSLLSTFSGIFNADSAQKGLSLLKNREGDMIASPIVTIV
                     DDPLLENGMASTPFDDEGVATFKKEVVLNGKLITLLHNLKTANKAGVKTTGNGIKSSY
                     SSPISIYPTNFYIDKGDKSLDEIIKDIDEGLMVTSFAGLHSGANSVTGDFSLAAKGFY
                     IKEGKKVFPVEQITVAGNYFDLLKDIEVIGEDLEFPMSSIGSPSVVIKELSVAGKDE"
     gene            complement(15195..16577)
                     /locus_tag="PC700_000515"
     CDS             complement(15195..16577)
                     /locus_tag="PC700_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HDF2579840.1"
                     /translation="MLSKNIASKVLEKCLITGGDFAEIFEEDTINNSISLIDNKVENA
                     IGGRNYGIGIRIFKGLKSIYAYTSDNSLNSLLDVAYKAAVALGKLEDGKSVILNDSIK
                     INNIHNIKIYPNSIGNRDKVSVMKVAYKSAKEFSNDISQVSVSYLDKDQKILIANTEG
                     IYVEDRRIRTRLGISSIASKGNENQTGFEGPGGCKGFEIFEEIDPEYYAKESARVAHT
                     MLHAKNCPAGNMMVAIDNGFGGVIFHEACGHSLEATSVAKGNSVFADKLGQQIASTKI
                     TAIDDGTIPNYWGSMNIDDEGNPTQKNILIENGILKSYMIDKLNGRRMGMNPTGSSRR
                     QNYKFAPTSRMTNTYIAAGEDRPEDIIKSIPDGLYAKKMGGGSVNPVTGEFNFAVSEG
                     YLIKNGEIQEPVRGASLIGKGSDILMNIDMVGNNVKQAQGMCGSSSGSIPTNVGQPMI
                     RVKEITVGGR"
     gene            16821..18035
                     /locus_tag="PC700_000516"
     CDS             16821..18035
                     /locus_tag="PC700_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDF2579841.1"
                     /translation="MNQEYVKGNPIFTNINKVPRQFPYLTDDIDTDVIIVGGGVTGCI
                     CAYYLAKNNIKSVILEKGRIAHGSTSVTTSLLQYELDDNLIDLTEVMTLKDALKAYNL
                     CTSALEELDTFIKLYGNKCDYAKRDTLLYTANKLEIKAIKEEYNLRKENGFDVEYIDE
                     STNPFSFDLKSGLIAKNGGRELDPYKYSHHLIDVSLKNGLQVYENTEVKKVDLSNDKV
                     TAEVSYGYKVHGKKLIVATGYNTSLFTKRNFATKSNTFNIATKPLKNIASWKNNILIR
                     DNCDPYNYLRTTKDNRLIIGGEDVSFDDIENETLANEKYDILEQRLKSMFKDIKDIEV
                     EYKYCGCFASTLDNLGFIGPDNKNNNLWYCLGYGANGILFAILGGIMLSELYLGKQNK
                     NMKLFKVDRFDK"
     gene            18212..20125
                     /locus_tag="PC700_000517"
     CDS             18212..20125
                     /locus_tag="PC700_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2579842.1"
                     /translation="MKYGGIIMKEILVIAPTKGTYEKSIHIVKKNKYTNIDVVFGNLK
                     EGIPLAEKSINHGTRIIISRGGTYNMLKATYNIPIVEIKVDAYDIIKSYKEVKNSNEP
                     FGIIGFNNVIYGFDIIEEILNKKITMIEIEKEEEIYDAIEKYRKKGINTYIGDTTVAH
                     IVKRLNCKGILIESREENILRAIQQAEQILEATKDEQKRRLQIEAMTDFVHDGIITVD
                     KNFKITLFNKSAEKIFGIKKENALYHNVVDVIPNTVLPQVVETMEPQIGEIQNIGSTK
                     IITNRVPIIVDGQIQGAVSTFQDSKEISSFEHTIRRSLLSKGLSAKYTFDNIIHASEK
                     TKDCIELAKKYACYDTPMIITGESGVGKELFCQSIHNYSKRKNAPFVAVNCAAIPPSL
                     IESEFFGYEEGSFTGARKKGKAGVFELAHEGTIFLDEISEIPLELQGRLLRVLQEKQV
                     MRIGGDKVIPIDVKIICASNKDLKNMMRNGLFRQDLYFRINILTLYLPSLKERKEDII
                     KLSEFFIHKYSSKYNKTPLIITPNIKKALLEREYEGNIRELEGMMERAVIVESFDSIL
                     LEKVSNEYTNNIVDKDNSDFECSNLDLKTLEKKYIYKIYNENNRNTTKTCEILKITRS
                     TLWRKLKEIEDDV"
     gene            20396..21370
                     /locus_tag="PC700_000518"
     CDS             20396..21370
                     /locus_tag="PC700_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HDF2579843.1"
                     /translation="MHILKSVKKIPGGLMVCPLLLGALFNTVCPQALEIGGFTTSLFK
                     TGAMSILALFCLCNGAQINIRQAGKPLTKGIVLTATKFIIGAVLGIIVSKFIGPKGII
                     GITPLAIVATMTNSNGGLFAALAGEYGDSTDVGAISILSLNDGPFFTMLAFGVTGLAN
                     VPIVALFAALIPILLGFILGNLDEDIREFLAPGTTLLIPFFAFPLGAALNFNQIITAG
                     LPGVVLGLGVTIITGMGGYFVMKLIRAEHPAVGAATGTTAGNAAGTPEALAAIDPTLA
                     TIAAVSTVQVAAAIIVTAICCPMLVSYLDKREKKKKSIFKTNKNIEVN"
     gene            21391..21666
                     /locus_tag="PC700_000519"
     CDS             21391..21666
                     /locus_tag="PC700_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDF2579844.1"
                     /translation="MNAIIHANEKDNLVTCVRPLVKGEKVSIDGKSYIVNDNIPVFHK
                     MATEDVKKGDVVYKYGEIIGIATVDIKTGDYVHVHNIESTRGRGDKK"
     gene            21679..22842
                     /locus_tag="PC700_000520"
     CDS             21679..22842
                     /locus_tag="PC700_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDF2579845.1"
                     /translation="MKLMGYLRENGQFGIRNHVLIIPTSVCSSETATRIASLVPGAIA
                     IPHQHGCCQIGSDIELTAKTLIGFGKNPNVAAVLVVGLGCDGIQAKELASEIATTGKK
                     VDYVVIQECGGTLKTVSKGTEIVSKMAREVSKEVRVEFGMSEITLALECGGSDPTSGI
                     ASNPSIGVASNLLVDEGGSSILSETTEVIGAEHLLATRFEDEEMKDKFLKFVSDVEKR
                     AIAMGEDLRSGQPTPGNKAGGLSTIEEKSLGCMYKAGNKPFKGALEYADIVPPDKKGL
                     YFMDTPGQDIDSITGMVAGGAQIVIFSTGRGTPTGSPISPVIKITGNSDTYNKMPDNI
                     DINAGRIITDGAKIADIGQEIFNEIIEVCNGKHTKAESLGHREFGIYRISSTF"
     gene            23179..25459
                     /locus_tag="PC700_000521"
                     /pseudo
     CDS             23179..25459
                     /locus_tag="PC700_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439637.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
     gene            complement(25554..26894)
                     /locus_tag="PC700_000522"
     CDS             complement(25554..26894)
                     /locus_tag="PC700_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2579846.1"
                     /translation="MENQEALRHEKIWILLFRYSIPAIIAMMVTSLYNVVDRAFIGSM
                     EGIGSIAIAGLGVTMPVFTLIIAFGMLVSVGASTRLSIKLGERNREEAEKILGNALTL
                     SIIISLIITILGLVFLEDILFILGASKDSIFYAKDYMSVILIGSIFNLVAFSLNNAIR
                     AEGNPKLAARTMIVGCVLNLILDPIFIFVFNLGIKGAAIATVLCQIVVFFWVTYYFIR
                     GKSTLKLKKCNLKLNKDIVKKVFAIGMTPFAMEVAISITHMFTNNSLKVYGGDLAIGA
                     MTALTSILLMFMMPVFGLNQGMQTIISYNYGAKQYERAKKTLILSIIVSVVILSFGFL
                     IVQVFPEVFVGIFNKDSDLMDIAVRGISINLITLPIMGISIVGPVYFQCISKVKHSMF
                     LTLLRQFILFIPLIIVLPIQFNLDGVWLAQPVADFIAMIIVLLFLKREFKTSRV"
     gene            complement(26906..27688)
                     /locus_tag="PC700_000523"
     CDS             complement(26906..27688)
                     /locus_tag="PC700_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2579847.1"
                     /translation="MNCINTKNLNVSYGNTDIIKNLNLNIPKGKITTIIGANGCGKST
                     ILKTIGRIISPNSGKIYINGEDIYKKKSKEIAKDMAVLPQSPQAPSGLTVSELISYGR
                     FPHKSGFGNSSKEDREIVNWSLEVTGIAEFKDRNMEDLSGGQRQRAWIAMALAQETDI
                     LLLDEPTTYLDLAHQLEILKLLDELNKKQGRTIVMVIHELNNAARFADHMIGIKKGKI
                     VCEGDAYDVMTKDNLKELFNIDAEIATDPRTNKPICITYDMV"
     gene            complement(27703..28725)
                     /locus_tag="PC700_000524"
     CDS             complement(27703..28725)
                     /locus_tag="PC700_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDF2579848.1"
                     /translation="MKNIIKINSKTLLVISILMVLIFITFLLSLNMGSFSIEPMDVIK
                     TFLGQGQRNHEIAIFKLRLPRIVIALLVGSALSTAGVILQGVTKNDLADSGILGINSG
                     AALFVVVYIYLMNGNVYEGMSNLTVFTMPIVALIGALFGAFLIYSLAWKGGINSSRLL
                     LIGIGVNIAFTSILTIFQLKFTTQEFNRVMAWTSGSIWGTSWKYVIAVLPFIVIFMGI
                     TIYKSKYIDVLNLGDEVSISLGVDVEKQRRRLIIYAVILSGVSTAVAGSISFLGLIAP
                     HIGRKIIGPKHKRLIPVSALIGTLLLLIADTISRNLLAPIEIPVGIVVSIIGVPYFIY
                     LMLVND"
     gene            complement(28731..29744)
                     /locus_tag="PC700_000525"
     CDS             complement(28731..29744)
                     /locus_tag="PC700_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDF2579849.1"
                     /translation="MTKLSKKNITYLLILVSIVVLVFGIVLSITIGAKDMNLSTVIDS
                     LIKMEDGINMRIVKDVRLPRAIAAALVGGFLAVSGAIMQGITRNPIAEPSVIGITQGA
                     TFAISISLVLQKKLPQLIHGSFSVMMFAFIGASISGLFIYFISSKSRGRVNNVKLALA
                     GVALGTLLISLASAISMYFNLSQQLSFWISGGLVGVKWEGIKLLFVAGGIGFILAIIM
                     APRITILSLGEEVAIGLGQKTNFVRFICIVLVILMTGASVSVSGNIIFIGLIVPQIAK
                     GIVGSDYKYIIPSSLVLGAVLLVYTDILSRMINPPYETPVGSITALIGVPIFIYLVRK
                     GEK"
     gene            complement(29872..30810)
                     /locus_tag="PC700_000526"
     CDS             complement(29872..30810)
                     /locus_tag="PC700_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2579850.1"
                     /translation="MKKIKSLAIFISIITTLVLVTACSDKNTEDKDKSETRVVQSVKG
                     EVKIPSNPKKIVDISGSSEELLLAGYKPVATANVDSYETDKLPSYIREELKGVKIVGH
                     SMMDTMDMEAILEVNPDLIIMSQRQEKIYDQLKEIAPVVMMKDYANDWRSKLTDVSKL
                     FDKEEEAKSWLQKYDEKATKLGKEVIEKNGEKTYLPVLASSGQFMVFSDGGIGTLIND
                     DMKLARPKNMPKQDGITLPMVSMEGLTDIDADHIVVIATEADKKDLENSAIWSQIRAV
                     KDGNVTILDAAPFFSQSYNPIGKELLLESVKNELTK"
     gene            31080..31544
                     /locus_tag="PC700_000527"
     CDS             31080..31544
                     /locus_tag="PC700_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2579851.1"
                     /translation="MVHLVYCDNAGKKGEKVLDKILAGTKTMVIRGAAGRKIPHSRVF
                     EDELLYFMEKGTGKISAKATVKKVENYVKLTEDEISKILNENQPKLNLSEKQKVRWHK
                     KCLCLVEFENVEEITPLDFDHQGNMDDWLIVEKIEDVVVGTSIPYNYEKSKF"
     gene            complement(31656..31973)
                     /locus_tag="PC700_000528"
     CDS             complement(31656..31973)
                     /locus_tag="PC700_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2579852.1"
                     /translation="MDEKMIEISFGIIGFAGDAKGLAFEAIKEAKSGNIDKARECIEK
                     SKESIHKAHKFQTELIQNEANGNKTEISVILIHAQDHLMTAMNFQQLAEEFIDVYERL
                     ESK"
     gene            complement(31986..32717)
                     /gene="chbG"
                     /locus_tag="PC700_000529"
     CDS             complement(31986..32717)
                     /gene="chbG"
                     /locus_tag="PC700_000529"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HDF2579853.1"
                     /translation="MTKIIINADDFGYCEAVNYGIISAHNNGIVRSTSMMANMPGVEH
                     GVGLLKENKTLNCGVHMTLSCGRPLLSNLKTIVDKDGFFIRRITDEIIEKMDCDEIYR
                     ELCAQIDRVKGLGIDISHLDSHHHIHTLVSLKPVVEKIVTKYNLPIRGGFEYNLEYSK
                     VVPLIDSFYKENVSEEYFIKNIDEIMKYDVVDIMSHPAFLDDYILNSTSYAIDRTKEH
                     KILTSKKVKEFLEKNGLVISSYRDI"
     gene            complement(32743..34053)
                     /locus_tag="PC700_000530"
     CDS             complement(32743..34053)
                     /locus_tag="PC700_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDF2579854.1"
                     /translation="MKKGLKIVTIGGGSSYTPELVEGFINRYEELPVKELWLVDIEAG
                     KEKLEIVGNLAKRMVKKAGVDMKINLTLDRREALRDADFVTTQLRVGLLDARIKDETI
                     PLSHGVMGQETNGAGGLFKALRTIPVIFDIIKDVEELCPNAWMVNFTNPTGIITEAVF
                     KYTNFRRYIGLCNVPIHLKNDVAKLFNVESDRISMDFAGLNHMVYGLNVSLDGEDVTK
                     EAIDKFVKADISMQNIKAIDFNAEFVKSLGAIPCPYHRYYYKTKEMLEDELREFKEGK
                     ARGQVVKELEEQLFELYKDEKLDVKPPQLEKRGGAYYSDAACNLISSIYNDKKDIQVV
                     NTLNNGSIRDFKDEQAVEVSSVITKNGPKPISIGYLPDSVHGLVSQIKSFEVLVAKAA
                     VYGDYESALLALCINPLIPSDDLAKTILDEMLEAHKDYLPRFNR"
     gene            complement(34130..35443)
                     /locus_tag="PC700_000531"
     CDS             complement(34130..35443)
                     /locus_tag="PC700_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2579855.1"
                     /translation="MEKFMSFMDKYIVPVAAKIGAQRHLVAVRDAFIVMIPITMVGAL
                     GTLINNLPLEAYKNLMASIFGENWTTFGGDLWWGAIGTMAVFLVIGVAYFLAKSYESD
                     GLQSGLIALSIFFIMAPQIGKIVPEGGTTVVEGWGMIQQTYLGTAALFSSILIGLLST
                     EIFVRLSKVKKLTIKMPDGVPPAVSRSFAKLIPGMLTIMIFTVIGIFIKMLSNGSFLT
                     DILNTYLGAPLSNVADSLGSTMLIAFIIHILWTVGLHGANIALPFTETILMKLGGENA
                     ALAQAGATEGYHVLAGAFFDAFVYLGGSGMVLGLIVALLIAGRRRKEMIVLGGPPAIF
                     NIGEPLIFGLPIVLNPIFMIPFVLAPVICSAVSYLAIDFGLVAPVILPKIPWVTPPIL
                     GGAMATGDWTGGALALFNLILSILIYIPFVIASEKMEANKLKINN"
     gene            complement(35465..35776)
                     /locus_tag="PC700_000532"
     CDS             complement(35465..35776)
                     /locus_tag="PC700_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2579856.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMEDAAKENGIEAKIWAISEVNLKN
                     EIENCDVLLLGPQVRYVLGKATEMAKPYNIPVEVINMMDYGRCNGKAVLDRAVELNSS
                     K"
     gene            complement(35976..36680)
                     /locus_tag="PC700_000533"
     CDS             complement(35976..36680)
                     /locus_tag="PC700_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2579857.1"
                     /translation="MKEPIYKVIENHVRELINSDSLKEGDLIPSEKQLSEEFNVTRMT
                     VRSALNNLVKEGYITRQRGVGSIVIANNIYDNISSVSGFTKEMESKGYKVSNILVSLE
                     IVQADENLSSKLNISLEENVWEIKRVRLANDARVSYMITYMPVKLFPNLNKTHCENSL
                     YNFVEEVCGYKIAMSEREVQAVISNEECMDNLKLEEPEPLLYISQICKLQNSEIFEYS
                     HTYHYGYTLTLNAVVE"
     gene            complement(36775..38136)
                     /locus_tag="PC700_000534"
     CDS             complement(36775..38136)
                     /locus_tag="PC700_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2579858.1"
                     /translation="MKDRINLTEGRITEKLLKLSLPIMGTSFIQMGYNMIDMIWVGKA
                     GSKAVAAVGTAGFFPWLAMAFIMISKVGGEIKVAQSIGANNISTTKSYMKSAIEINII
                     LAIIYTISLIVLNKQLIGFFRLGDLEVITMSRQYLIIVALGMVFYFINPVFTAIFNGL
                     GNSKTPFRINTVGLITNIILDPILIFGWGFAPKLGVAGAAIATVFAQIVVTTCFIYII
                     VKSKEEYFKLRLFKEIETKYYKVLYKLGLPIAIQSGMFTVFSMLLGVIVASWGPVAVA
                     VQKVGSQIEAISWMTAEGLSVALGSFVGQNYGAKKYSRINKGCKIAITVSSVLGIITT
                     LVLVFAGESIFSIFIDESEAIEKGAIYLKILGYSQFFMCLEIITTGAFKGLGRTYIPS
                     IIITILTGARVPLAYFISRPEMLGLNGVWWSITLSSILKGILMISLFISLLKLGKLYK
                     MSE"
     gene            38635..>39586
                     /locus_tag="PC700_000535"
     CDS             38635..>39586
                     /locus_tag="PC700_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2579859.1"
                     /translation="MKINKKFNRVDMQVSILTAIIVIISSSCVYFFNYTLTYKSMIYS
                     LSERSKHIYEYVERNIDKNTFTEINSKEDQSKKSYKSSKILLESVKNTTGVMYLYTAK
                     KTKDGSLVYVVDGLNSSRSDFRNAGDLIEKEIWGDLNKALDNHILLPKKIKSTSWGYI
                     FISYFPIHNDVGKVVGVIGIEFEAKHQYNTFKFISIVTPIIALLTCLISALIAVILFK
                     RISNPTYTDFANTDYLTGLKNRNAFEIDMNNLNNSNLKNLISIISIDLDGLKKINDKF
                     GHQTGDLYIKQASKIIQDVINKKYIVYRIGGDEYIIILKNL"
BASE COUNT        13692 a         6685 c         4826 g        14383 t
ORIGIN      
        1 acgaagcatg atttttattt aaataaatcc caaacttcgt ctttacttac ttagatattt
       61 atttactttc taaatcctat tatttttaaa accttatctt tttaatactt caccacattt
      121 attctcagta atctctccat tttctaacgc aatttttcca tttacaatta catattcaac
      181 accattggct agtaattgtg gctttgaaaa atcaatatct tctgacctag aataactata
      241 gttatccatg tcaataactg ctatatcagc tatcaaccct tctttaataa tacctctatt
      301 ttttatttta aattgcctag ctggtaatga agtaactctt cttacaatct cttccaatct
      361 aacaccagac tttctgtaca tattgaagaa aattggaaaa gctcctcttc tttgtagttc
      421 aaaccatgac atatccccac ccacactaaa caaacaatct gaaccaacca taacaaatgg
      481 attatcaatt attttattat catgtagttt atctgggaaa tcagctctat acattcctcc
      541 taaacaaaag ataagttctt cgtctccatc taaaagtaaa tcgagtaata acttgtcctc
      601 atctattcct ttttgtgtag ctatttcacg tattgatttc ttttccatat taatatcttt
      661 agtgtttaga agtattatac tccccctatc tccaagtata aatgcttctt ttaatataag
      721 tgttcttccc tcttcatttc taagatcatc cattacccca tctataccta attcaaatag
      781 actagatgat attgccataa taaattgaat tactcttttt ttcttcatac aatgcccaga
      841 acttcttggt actgtgtcaa ccattatttc aacaccatta tacctagcct ttaatatctc
      901 atcaaatgct tctatttcaa ctggacttaa gtgagatact tgaaccctag caccactttt
      961 ttgcccaacc tctatggctt cttttactgc attatatctt cctatgtaat ctctaatatg
     1021 agatgtatat ataccatcat gctctttaat ctgccttgca atatcaacaa gttcatcaat
     1081 atcagcatac ttacttggta tatatgccaa tccagtagat attccaaaag caccactttt
     1141 cattccatca tttattatat ctgtaatttc atttttttcc ttttctgtag gaggtctatc
     1201 cttagaacct cccataacac tccatcttat agtcccatgc cccaacaaga atcccatatt
     1261 gacagaaata ccacttttag atataaggtc acaatattct gtaaaattat tccaacattt
     1321 atttttatct ataagatact ttttttcttc ttcaagtaat agaccatttt tatacatata
     1381 ttcatataca ttttcagaag aatatggtgt tatagaatgt ccacaattac catttatagt
     1441 tgttgtaact ccttgtttta gaaacttttc aaatttacca tctagtattg ctactatttc
     1501 ttcatgaaca tgagggtcaa taaatccagg tgttataact tttccacaac aatcaatctc
     1561 tttatttgta ttgctattta tcttagaatc tatttttgct attaagttgt ctttaattgc
     1621 tatatctcca taaaaaccag gattacctgt accatctatt ataaatccat tttttaagat
     1681 taaatcatat atcaaaatat tccctcctta aaaaccaatt tttaaataat cttaatgtaa
     1741 tttttatatt tgaaaatcaa attattatat ttcatatttg aaaattcact attatttcat
     1801 tgtaattagt cactaagctt atctttatac atagctcttg caattaatat agtaccaaat
     1861 acacatataa gaactactag agactgtggt attatacctt gctttgataa tataatagtc
     1921 aatacagata ttacaattgc tactagacct aattttttat cttgtaaaaa tacttgtcca
     1981 aaaactgacc caaatagagc tggtaaaatt aagtttaatt tttctactac ttcagctgga
     2041 attttagata atacgcttcc tcctaaaatt acccctatag ttagtatact tatattcact
     2101 aaaatagata cagctattcc tattgtagat attatcgagc cttcttctgt acctgattca
     2161 acttctgctg ccttttgagc tgctattgaa catggtagcc tcatattaga tatattacca
     2221 gaaagaaaac tcatataagt tcctgggata cctaagattg gtgaataaga aactggttct
     2281 attatgtaat ttggagcact catactgaca attgccattg tacttgctaa taatgctgat
     2341 attggaggca taataccaaa tccaaagctc aaaattattc caggaactaa tgctgctata
     2401 atacctaaag atagtgttag tcttccatac cttatcataa aaggtaaata ttcttcattg
     2461 tatatagatt ttactttact cataaaactc cccccttata ttactgaagc tattatcatt
     2521 ccgctaaaca ttgatattgt aagcccccac tctcttaacc attttatatt tttcttcttt
     2581 tcaagcatac acaatattac cattatagcc attccactta tacatgcaat tgttccatta
     2641 tcaaaacgca acaccctatc agcatttaga tatgcaaatg aacccaacat tgctcctaga
     2701 gatattattg gtataaatga tttcttgcca ctagatataa cattggttat cttatctagt
     2761 ttatgtccaa atagtaatgt gaatattatc catcctaaag aacctaatat catagtccaa
     2821 actgcatttg taaatgcaag ttttgtcatt tcaggagaac ctaaagttat acctaatgca
     2881 tctgttccaa atccagctga cattgattca aaaattacag aacctataaa tgataatctc
     2941 atccatgcta taggcccacc catagttact ataagcgata ccattccagc aaatatagtc
     3001 attgaaggcc caatagctga aatagcacta ctcttaacag ctgactttat ttgtttatct
     3061 gttattccca ttgttttacc aactttgcaa gattttatag caaatataag ggattgtacc
     3121 actactattg atactgctac tgtactcgct atccacaaaa gtggatgatt agctaatttt
     3181 aaataatcca tttataaaac ctccctataa atcctcaaat tttattaata tttctaatat
     3241 atctatttcc aaacctgttt tagtactcta agtatatttc cacctagtac cttctctatt
     3301 tcctcatcac tatatccatg ctttactaaa tatctgacaa tgttttttga agtttctgtt
     3361 gggttctcaa gaccttttac atattctact ttgggatgtt cttttgtacc tgttattttt
     3421 tttatatcaa atttagcagc tagtactgag tgtaatttta catggtcgcc atatactgta
     3481 tctggtccaa aagctacatg gtctattcct actaaatttt taatatactc aaaatgttcc
     3541 ataaatgact ctattgaatg ttctttattc ttctctgtta ttgtagtatg aggtgctgct
     3601 tctacaccta taacccctcc ttttttagca catgctatta atacttcatc tggagttagt
     3661 ctctttgtgt tccataaatt tcttgcccca acatggctta atattattgg gtccttgcta
     3721 tattctatta catcaagtgt agtttgaact ccaacgtgag agcaatctat tgccattcca
     3781 attttattca ttctttcaac agcttttttt ccgaaatttg taagacctcc atctttctct
     3841 tcttttagtc ctgaacctaa tgcattggat tcgctgtatg ttatacccat taaccttata
     3901 ccaaatccat acagtatatc tattctatca agttcatttt ctattggtgc tgcaccttct
     3961 aatgttggta tcaatgctac tttaccttca cttttagctc tgcatatatc ttctactttt
     4021 tcacacttta tgacaaaatc ttgatgagct aaatcacata gtctcatgcc taaatctatt
     4081 aatatatcat cccatttcca ccctgaattt gatgttatcg tacatacacc atccattaaa
     4141 ttatcaaata tagcatcata atatcctctt gataatgctt cataagcgca tctttgttta
     4201 ccttccctat tatattcaaa tatatctctt tctaggtgtt ctggaaataa tactggatgt
     4261 tcatgtaatg atataaatat tttttcatcc actatacttt taaccttagc ttcttgaata
     4321 tcatctaatt caataaggta ctctttaact cttttatctc ctttacacat ctcaatagat
     4381 gaaatatctt ttcctttttc taaataacta taggcttcat atcctttgta agctgacttc
     4441 ataacaacat cctccaagtt ctacaatttt ttatattttt cctatgtagc aattgatatg
     4501 ccaaaatata aattttaatt gtataataaa tcttgcaaaa aataaaaaca gtagtaaggt
     4561 tgatatttac tactgtttta ttaaaaataa tcttcttaga ttatttattc aatatttttg
     4621 aattatctta atcttaaaga taattttatt tatttctatc cattataggg atttttttag
     4681 ggtgttttgc ctttttagtc ccctatatca ctcccttaat ttttacatat gatataaagt
     4741 tctttccttc atcagttata aaactacctt gtttttttac tccagataaa agtaaaccat
     4801 ggctctttaa cttatctatt ctttttctaa tttggtctac acttaaaact atatcttttt
     4861 cttttaaaat ctcttgcagt ttatttcttc ctaataatat atttttatta ttccaagttt
     4921 ctatactgtt caatatacat atactttcct caaagaaatt agattgtttg aaatctaaga
     4981 taattgaatc gaaattttca ttaactaaag tattgttttt ttcaaatcta aattgttctg
     5041 gcaaatgctc gtaatctact ctatcttcct caactatagt atcaatataa taaattaaat
     5101 ttttaagctc tcttacattt cctggccatt catacaatgt taaagcttgc attgattcct
     5161 ctgtaaaaca tttgttgcta tttatttcat ctaaaaaata cttacttata agaggtatat
     5221 cttccttacg ttctctaagt ctaggtatct ctatatgtaa tacattaatc ctataataca
     5281 aatcttctct aaaagaacct tctttaattt ttttctttaa atctttattt gttgcagcta
     5341 taattctaac atctattggt ataattttac ttccgcccat tctcataact tctttttcct
     5401 gtaatactct tagcaatctc tgctgtacat ctaaagatat atcacctatc tcatctaaaa
     5461 aaattgttcc tgtatgcgct ctttcaaata tacccatttt tccacctttt attgctcctg
     5521 taaaacttcc ttcatcatag ccaaaaagtt cactttcaat aagtgtatct gacaaagaag
     5581 atagattaac tgcaacaaat ggcttgtttt ttcttagaga ttcattatgt attgcctggg
     5641 caaatatttc ttttcctgtt ccattttctc caagtatcaa cacagaaaag tctgtagttg
     5701 ctatttttct tgcaatatta attttttctt ttattatttt actctcacct acaacactct
     5761 caaatgtata ctttgataca aaaccttttg actgaagttt attttgaatc ttgtcctcaa
     5821 gcacttgaat tgcacttata tttttaatgc ttataatact ctttattcgt tcattatttt
     5881 cataaacatt tactttatta attatcagtt ttttgttatt taaatttact acttcatcat
     5941 taacttcatc ttcttgaaaa aatatttttt tgacaacctt atcactaaat aagtccataa
     6001 agttattaga tataatttca ttttggtcta ttccaactag attagaaaat actttgttgc
     6061 aatatataaa ttttcctaat ttatcaacag aggcaatacc ttcatctata atctccaaaa
     6121 aactcttcat ggttttattc atagttgtat aatacctata tccacttact gtatcttcat
     6181 tgtatttttc cttaataata tgtaatttat ctatagatat atttagtttt gtaaagattt
     6241 ctataactgt atttatatca attaccttat cacctatgtc aattatttgc tttatgtttt
     6301 gtggtatgct gtttctgctt cccgtaataa ttccaatctc acatttagac ttatcacaac
     6361 ctggataata tggtattaga ttaatatgaa ttaatcctag ttttctaatt atcttggcag
     6421 tttcatctgc tgactcttta taagcatcta taaccataac atcagaatca ttttctatac
     6481 ttattatctg atttatattt tcaatattta tagtacgatg tactattaca ataagtatat
     6541 ttttatcaat attattatgt atatattcct ttatatcatt gtctgaacat actacaagct
     6601 catagcttga cagttcaata tttatcctat ttacatatac caagttatct atattacaaa
     6661 attctccaaa tatattttcc aattgttttg atatatctat atttattttg tcatcagttg
     6721 atacaaataa aatattatgt tttttcatag tcccacctcc tgtttaacga gtatagaaag
     6781 taataatttg gtctattgta gcacttttta aaaaagtttt aaatcttaaa aatcaaattt
     6841 tgtaccattt actattattt tccataataa aataatgtaa gctatctttt ttcacatata
     6901 atttaatttc taaaacggtt agtttgacat tattggaata taaacaaaaa tttaatataa
     6961 attcgttaat atattaatac atttttaaga atgacaagga ataaattata acaaatttta
     7021 ggaatatata ataatagggt ctcagaactt agttacaagt tctaaaaccc tatttgttta
     7081 gtgtaaaaaa catcataaaa tttagcttaa atcttttcac ataatattac tctttttcta
     7141 agtgataata aaatatatct attcctctaa aaactgtaaa aattcctgat taaatagttt
     7201 aagctcatca tagaatatag catgaccact atactcaaaa gtatataaaa ttgaatcttc
     7261 tattttttca ttcataaaaa ctgcaaattc atacggacat atttcatcta atttcccatg
     7321 aaatatacca gtaggcactc ttacacattt taaatcatta aataattctt catctcttaa
     7381 agatacagca gttccaatag tgccaatacc tgatgcacta aaaccaatat cattaaacca
     7441 tcttctaaag ctttctggtg gatttgatgc aaatactttt tctccaaaat cggttaccat
     7501 ttctggtcta tcagtacatg cttgtgctat caatttattt acatcttcac gtgtcatacc
     7561 atatggaaat tctggaggtc tttgtacaaa tgatggtgca gcagctgcag ctaataccaa
     7621 ttttgacact ccatatccat taaataaact catataccta agtacgattg ctcctcccat
     7681 tgaaaaacca acaaaggtaa agtcttttaa tccaatagca tgtaccacct tataaatatc
     7741 gtctgctaat tggctataag tatatcctcc tgatgttgca tcagatttac caaaacctct
     7801 taaatcaatt gaaactgttc tatatccgag ttttggcaat atatttgttt gatattcaaa
     7861 tatcttatgt cctaagggcc aaccatgtat aaaaagtaca gtttttttag cactcggatt
     7921 caaatcatat actgctatgt ttatatcatt cacatataca taatacatat caacagcaac
     7981 tcccttcaag aaaatgtatg atggttattt tatatatatg aataaaatga tgaataacta
     8041 aatttttaaa tttatttaca atacttctct attgcctttt caaataaact tacatgaagt
     8101 ttttcatcta ggattattct atttaatata gcttttatat aagggtcttc aataagactt
     8161 atatctttat aataattatt aatagccaca tgctccaaat gcaaatctag ttttaatctg
     8221 ttacaaatag aatttccata acatacaaaa ccaccattcc aatatgcacc acaagaactt
     8281 gtagaacctc tatatacagg atttccaccc aataatctta tagtctttgc taatatctcc
     8341 atatgtaaca tttctgttat agatatattt atccacattt cacttaactc tctatgcgtt
     8401 tcatctaaat caaaattatg gtatagatat tgatttacac tcgtaaattc acttgatact
     8461 ccggcatagt catccatcaa taattctgca taatacttat ttagacctaa aacttttatc
     8521 tctggatatg gctcatcact tgaatatcca tttcgcttgt gcatgtcgta atgtgcatca
     8581 ctcataatat ctcctccttt aaatttaata cttgatatta attgtatgca tcacgacttt
     8641 ttcatataac ttagataata aaatctatta tattattttc tattataatt tatgtgtcaa
     8701 ttcacccaaa tttatttgat atgcaatatt tctttcctta gtgctacata tgtaataaaa
     8761 acatatatac aatatattac tacaaaatta ataagcgtaa tggctacagc atgtaagata
     8821 ggaatctgaa tattaaaaaa tactggagca tcaaaagaaa tattaaacat gttgtatgaa
     8881 aaaactgata ccaatattcc accaaatatt actgcaggaa ttaatggaat cgaaaaatat
     8941 aaacaaacat gtttctttaa gatttttatt acttcactat caatcatccc caaagttcga
     9001 agcattccat agtgttttat gataatgtta ttttcgctca ataattgaat agagagaatg
     9061 actgtaccaa taatgcatat aatgagtgcc aaataaaaaa gtggtaatat caagataatg
     9121 gcttgaccag tagattgcat agcatacttt ccacttatat aatccttatt atcatatcgt
     9181 aatttagaaa caaagccttg attacctgct actactactc cagaattaag attttctaaa
     9241 gtatcaaact ttttctgaaa ctcagtaaaa taagcactat ccaaaggctc acttgtaata
     9301 gcaacataaa gactatacag aggtttcata ttatcaatta aataatctgg aatgacaata
     9361 atatattctt gtccatttcc atatcctcca tactgtgaaa agttctctat ataaatacct
     9421 gcaatactta agtccttatc tgatattgtc agtttatatt tttctccaat ataattctta
     9481 tatgcatcag caagataagg catacaatgg atgataaatt gatttttgtc catctttatt
     9541 gggtttaaac ccaacatatt tcttagtata ttgtaatcac tatatttcat aaatgtgtca
     9601 aactggtttt ctgcaaaagt ataatcctta tattgtaaat ctcgccctat atctttcaag
     9661 tatttagaaa ggatattatt acgaaccttc ataaaggatg ttttttcttc tgtgtaaaga
     9721 gtataagaat aatccttttt cacacctttc gtttctgata aatatccttc atatgaagaa
     9781 aaatcatatg attcattcgg gtgaaaaata ctcacatcaa attgttgtaa atccactgat
     9841 ttattcatca cagaataaaa tgagttagct attccaatac aagcaattga tactgtaaaa
     9901 agagttgcga taatacccat tataatgctt attccattta tctttgaagc cagtagacga
     9961 tacaaaaata agtgagattt cttatatttc caattcatcc ctttttcaaa catattctgt
    10021 gcaatagatc caaatcccat aaataatacg attagacaaa atacaatcaa caataaacca
    10081 agaaatacat ctgttaatct accactaggt attgaaaaga aaaaaatacc catacctcca
    10141 cacaaaacag acacacaaaa cataatagac ttcgtttttg tttggtgaat taatacattt
    10201 tcattatatc gatcataatt aagcaaatta attaatttca ttttaacaag ttttttatta
    10261 ctttttaatt gagcaattaa atatattatg cagaagtata aaaaagtcat cccagcagct
    10321 tttaatgaaa aatccggaga taaagaatac tttccatgaa acatattaat aacaatagat
    10381 tccaaaacct tagaaacaaa tatccctaga atccatccaa caataagagc agaagcacct
    10441 aataaaagat tctctataaa aagcatttta acaatttgtt ttttatctac tcctaatatc
    10501 atataagtac ttatttctct gcttcttttt ttcaatataa aattcatcat ataactaaca
    10561 aaccagccta aaataaaagt aataatgaac gaaaatatga taatcatata agaaaactca
    10621 tcgcttcctc cattattaag tatactagaa agttcttgca ttacgtcgga aaatatcaaa
    10681 gcgttaaaag cataaatcaa agctacagag cctattaagg aacaccaata aataaggtac
    10741 tttcctgctt gtcgtcttgc attacgaaaa gataatcctt tcaacataat caatctcctc
    10801 ccatcaaaga aagtgcatcc aaaatttcgt gatagaaagt aagtctggat ttttctcctt
    10861 ttctaagttc gttaaaaatt ttcccatccc ttagaaataa aattctctca gtataactag
    10921 ctgaaatagc gtcgtgtgtg acaagaagaa tcgtagcctc taatatggtg tttatagttt
    10981 ccaaaagttc cattaaagtt ttagatgaat tagaatccaa tgcaccagtt ggttcatctg
    11041 ccaaaataag tttgggctct gttacaactg cacgagctac tgcacaacgt tgcctctgac
    11101 caccagatac ttcataagga aacttcgaaa gtatctcttc aattttcaat tgtgtggcaa
    11161 tattatacac actattagta atttcaacag gagaaacttt ttttattgta agtggaagtg
    11221 ctatattttc ttctaatgtc agagtatcta ataaattaga atcctgaaaa acaaaaccta
    11281 aattatcacg acgaaaaaca gttaagtttt cttcggatag ttcagtaata tcttgatttt
    11341 caagataaat ttttccagca cttactgtat caattgtagc aatacaattt aacaaagtag
    11401 ttttaccaga gccagaagtt cccataatac tgacaaactc tcctgtatta atttgaaatg
    11461 acacatcatt caaggccttt gtaagtattt tctgattgcc ataatatttc tcaatgttct
    11521 ctactcgtaa tagcatgtta aagtcctcct tagtcaacaa tttttccatt tttaattatt
    11581 attgtcttat ctgaaaaatt caagatatat acatatatgc tttctttaga gtcttgaatt
    11641 tgaatagtag gttcattaga tgaaattaga gattttttta cttcaatatc gtattttatt
    11701 gttaattcat tatcatctcc atttttatta gtatcttcta catttttatt taaatcaatg
    11761 ttattatgat taaagggacc agtaaacttt acttctgtaa tagttccatt ttttctaata
    11821 aaagaagcat attcagacct tagtggtatg atacttcgtg agaaaaaacc catcgaatcc
    11881 aatcctgtaa ttgaaataaa caaaacgagt acaacacata atatagttaa tatccttttt
    11941 ttcatatgtt tctccctcct ttctattgat attgtagcgt aaatagtaat ctgaatatat
    12001 agaagttact taactttatc ttacagtttt gaaagataaa gaaatagcag cgcattttta
    12061 gttgcactgc ttctgcatat tgatagctgt atttattggg aaattcaatg ttattgctgt
    12121 gtattcacca aggctggagg acacatctat atcaattccc attttcttag acagttcata
    12181 acaaaggtaa agaccaatcc ctgtagaatt gtttgtagtc cttccattac tgccagtaaa
    12241 acctaattca aaaattcgat aaatttcact ttctttgatg ccaataccat tatcttttat
    12301 agtaaactta acactcttgt cttcttttat tgcaaaaaat gaaatttcag ctgaatcact
    12361 tttgtactga acactattga taataatttg atttaaaata aaaactatcc actttttatc
    12421 agtatataca ctatactcca aatcctgtat attaatatga atattatttt gtataagaag
    12481 caatttattc tttgctaata cttcttctac aattgacttt aatgttattt cagaaattaa
    12541 ataatctttt tcaacatggc caaggcgagc atagtacaat gcacgctcaa tatgacgttc
    12601 tatttcttcc atttgaaaac gaatttttcg tgtatgttca tttttataat tttcacatat
    12661 aagttctgat gttgtaacag gatgttttat ctcatgtacc cattgttcaa tgaattgtct
    12721 atattcgtac atttcacgat gtatttgaga aacatgatct gtcatagatt tcaatgataa
    12781 tctaataata ttgtagtatt cacgttctat gttagaagta ggtttttcaa tcatttcaat
    12841 aaacagatat tttttatcca attcgtctac aatgcgacgt aacttaataa tttctttttt
    12901 ctgtttttga taacaaaaac atatccaaca aaataaaatt gcgacccaag cggctataag
    12961 aagagtcaac tctccttgac caatccctat aagatgaaga tatagcacta atgaaattgc
    13021 acaacaaata tttaagagta taactaatac tttatcttga agaaaattac aaaacctcat
    13081 atcctatatc ccatcccccg ttttgtaatg attaagtcag taatacctat accccttaac
    13141 ttttctctaa gccttgtaat gtttacactt aaagcattat catcaatatg aatatgattg
    13201 tcccataaat attctaccaa atctatacga ggcacaatac tccctacatt ctgaaacaaa
    13261 taatacatga tttttagttc agttcttgaa agttctataa cagcattttg gtattctact
    13321 gtagctgaaa taaggttgag tataatctct ctatatttaa ttttctgttt atctgatgaa
    13381 tctactcttt taagtaggtt ctttaccctt gctaaaagca aaggtaagtt atatggtttt
    13441 gtaatatagt catctccccc catagtcaaa ccatttagtt catcaattgc actatctctt
    13501 gctgtaataa aaataatagg tatagtagaa caattcctaa tctgtctaca taaagaaaaa
    13561 ccatctatat taggcaaatt gatatccaag agaattaagt caggtgttga ttcttggatt
    13621 acttgaagta catcacaatt atttgtatcc aatgtttgtg tactatagcc attattttca
    13681 aagaattgca ttaattcctt acagatgaac acatcatctt caattatcaa aatattggtt
    13741 aaaataataa atacctcatt tctttagttt atattgcttt aattttgtaa gtaatatatt
    13801 tttccatata ttacttacaa aattactttt taattattca tcttttccag ctactgatag
    13861 ctctttaata acaactgaag gtgaacctat actactcatt ggaaattcta aatcttcccc
    13921 tataacttca atatctttca acaggtcaaa atagtttcct gctactgtaa tttgctccac
    13981 tggaaatact ttttttcctt ctttgatata aaagcctttg gcagctaaag aaaagtctcc
    14041 tgttactgaa tttgcacctg aatgaagccc tgcaaagcta gtaaccatca atccttcatc
    14101 tatatccttt ataatttcat ctaaagattt atctccttta tctatataaa aattagttgg
    14161 atatatgctt ataggtgatg aatatgaaga ttttattcca tttcctgtag tctttacacc
    14221 tgccttatta gccgttttta agttatgaag taaagttata agctttccat ttaaaacaac
    14281 ttctttttta aatgtagcta ctccttcatc atcaaaagga gtagatgcca taccattttc
    14341 taaaagtggg tcatcaacta ttgtaactat aggagatgct atcatgtctc cttctctatt
    14401 tttcaataaa gataatcctt tttgagctga atctgcatta aatatcccag aaaatgtgct
    14461 aagcaaagat accatagctt cattaagcaa tatagtttta tatttacctg atggaatact
    14521 cttaccacct actttagata aagcattatc aacaccaagt tttgatatct cacaaggttt
    14581 tatttcttca atagaatttg ctctattata tcctattcca tcatattttt gtccattgtc
    14641 ttctatgata ggaactacaa accctataag catattagtt ttattgctta aattaagacc
    14701 tttagtatta gatataccat tacttgatac agaataagat atagtacatt tagataaatt
    14761 taccacctta tctgaataag ctttagtctc tctttccatt tctaaagcta aatcaatcaa
    14821 cttatctgcc tctaaatttt ctagctcttt agaataagtc tttacatcat tatagtgctt
    14881 atctccttca tatataaatt gtacatcttc attttcaata gtgtttactc catcttttac
    14941 attttttatc aacatatcaa cagctttatc atctaaaatt tctgtataag aataacctat
    15001 ctttccattt accttacctc taaaagataa accaaaagat ttatcaagat tgtatttctc
    15061 aacttctcct tcatatatat ttatacttaa attttctcct gtataataat atatttcaca
    15121 ctcttcaaaa ccttcactta gagccttttt aaataatata tctttaaaat ttttaagttc
    15181 cacaattatc cctcctatct tccacctaca gtaatttctt ttacccttat cataggttgg
    15241 cctacattag ttggtatact tcctgatgat gagccacaca tcccctgagc ttgttttacg
    15301 ttattcccaa ccatatctat attcataagt atatcgctac cttttccaat taaactagca
    15361 cctctcactg gctcttgtat ttctccattt tttattaagt aaccttctga aactgcaaaa
    15421 ttaaactctc ctgttacagg atttacagaa ccaccaccca tttttttagc ataaagtcca
    15481 tctggtattg actttataat gtcttctggt ctatcttctc cagcagcaat ataagtgtta
    15541 gtcatccttg aagttggtgc aaatttgtag ttctgtctac gagaacttcc tgtaggattc
    15601 attcccattc gtctaccatt aagtttatct atcatataag acttcaaaat tccattttca
    15661 atcagtatat tcttttgagt tggatttcct tcatcatcta tattcataga accccagtag
    15721 tttggtattg taccatcatc tatagctgta atctttgtgg aagcaatctg ttgacctaat
    15781 ttatctgcaa atacagagtt cccctttgca actgatgtag cttccaaaga atgtccacaa
    15841 gcttcatgaa atattactcc accaaatcca ttatctattg ctaccatcat attacctgca
    15901 ggacaatttt tagcatgcaa catagtatgc gctactctag ctgattcttt tgcataatat
    15961 tctgggtcta tttcttcaaa tatttcaaac cctttacaac ctccaggacc ttcaaatcca
    16021 gtttgatttt cattaccctt agatgcaatt gaacttatcc ctagtctggt tcttattctt
    16081 ctatcctcaa catatatacc ttcagtgttt gcaattaata ttttttggtc tttatccaaa
    16141 tagctaacac ttacttgaga tatatcatta ctaaattctt ttgcactctt atatgcaacc
    16201 ttcataacac ttactttatc tctatttcct attgaatttg gataaatttt tatattatgt
    16261 atgttattta ttttaatact gtcatttaat attacacttt ttccatcttc caatttacct
    16321 aaagcaacag cagctttgta tgctacatcc aacagactat ttaatgaatt gtcacttgta
    16381 taagcatata tactcttcaa tcctttaaat attcttattc ctataccata atttcttcca
    16441 cctattgcat tttctacttt attatcaatt aaactaatag aattatttat agtgtcttct
    16501 tcaaaaatct cagcaaaatc tcctccagta attagacact tttctaatac cttagaagct
    16561 atattttttg aaagcataaa tttacctcct actttaaatt tttaattaac ttttattcaa
    16621 tctttttact tgttaacaaa cattatatac tatattactt aaaataaggt aattatgaat
    16681 atattataca tatgttttat actaaaatgt tatcacattt tatctataca tacccatata
    16741 actagataat taaaatgtat aatattattt gctatagaaa ataatacact catacataat
    16801 aaattttagg agtgaagtat atgaatcaag aatatgtaaa aggaaatcct atttttacta
    16861 atattaataa agttccaaga caatttccat accttactga tgatattgat actgatgtta
    16921 taattgttgg tggtggcgta actggatgta tttgtgctta ctatctagct aaaaacaata
    16981 taaagtctgt cattcttgaa aaaggaagga ttgcacatgg aagcactagc gtgacaactt
    17041 ctctattaca gtatgaatta gatgacaatt tgatagattt aacagaggtt atgactctaa
    17101 aagatgctct aaaagcatat aacctttgta cttcagcact tgaagaatta gatacattta
    17161 taaagctata tggaaataag tgtgattatg ccaaaagaga tactctactt tatactgcca
    17221 acaaacttga aattaaggct atcaaagaag aatataactt aagaaaagaa aatggatttg
    17281 atgttgaata tattgatgaa tccacaaacc cttttagctt tgatttaaaa tcaggattga
    17341 tagcaaagaa tggaggacga gaattagacc catataaata tagtcaccat ttgattgatg
    17401 taagtttaaa aaatggtctt caagtatatg aaaatacaga agtaaaaaaa gtagatttat
    17461 caaatgataa ggttactgct gaagtatcgt atggatacaa agttcatggt aaaaaattaa
    17521 ttgtagctac agggtacaat acaagtttat ttacaaaaag aaattttgca actaagtcaa
    17581 atacttttaa tatagcaact aaaccactta aaaacatagc ttcttggaaa aacaacattt
    17641 taattagaga taactgtgac ccatataatt atctaagaac tactaaagac aatcgattaa
    17701 ttataggtgg tgaagatgta agttttgatg atatagaaaa tgaaacttta gccaatgaaa
    17761 agtatgatat tttagaacaa agattgaaaa gtatgtttaa ggatataaaa gacattgaag
    17821 ttgaatataa atattgtggt tgttttgctt caactttaga taatttaggt tttattggtc
    17881 cagataataa aaataacaat ctatggtatt gcttaggtta tggagcaaat ggtatactct
    17941 ttgcaatatt aggaggtata atgttaagtg aattatatct tggcaaacaa aataaaaata
    18001 tgaaattatt taaagtagat agatttgata aatagatatt gtatattaaa agactataaa
    18061 aagatgaaac tttataattc taaaagttaa ctttgataca gtcttttata atgttccttt
    18121 taaaaatact ttatttataa ttaaatataa agtaaaatgt taaaatataa ctgataaaat
    18181 ttagtcataa gataatattt aagtttattt gatgaaatat ggaggaatta ttatgaaaga
    18241 aatactcgtt attgctccca ctaaaggaac ctatgaaaaa tcaattcaca tagttaaaaa
    18301 aaacaaatat acaaatattg atgttgtatt tggcaatcta aaagaaggta tacctcttgc
    18361 tgaaaaaagt attaatcatg gtactagaat tatcataagc agaggtggaa cttacaatat
    18421 gttaaaagct acttacaata tacctatagt tgaaattaaa gtagatgctt atgatattat
    18481 aaaaagttac aaagaggtaa aaaattccaa cgaaccattt ggaataattg gatttaataa
    18541 tgtcatctac ggtttcgata ttatagaaga aatattgaat aagaaaataa ctatgattga
    18601 aattgaaaaa gaagaagaaa tttatgatgc tatagaaaag tacagaaaga aaggtataaa
    18661 cacatatata ggagatacca ctgttgcaca tattgttaag agacttaatt gtaagggaat
    18721 attaatcgaa tctagagaag aaaacatact tagagccatt caacaagcag aacaaatact
    18781 cgaagcaacc aaggatgaac aaaaaagaag attacagata gaagctatga cagattttgt
    18841 acatgatgga ataataacag ttgataaaaa ttttaaaata acattattta ataagagtgc
    18901 tgaaaaaata tttggtataa aaaaagaaaa tgcactttat cataatgttg tagatgttat
    18961 cccaaacaca gttcttccac aagtagttga gactatggaa cctcaaattg gtgaaataca
    19021 aaatataggg agtacaaaaa ttattacaaa tagagttcca ataattgttg atggccagat
    19081 tcaaggggct gtttcaacat ttcaagatag caaggagata agttcttttg aacatacaat
    19141 aagaaggagc ttattaagca aaggtctatc tgctaaatac acttttgata atataattca
    19201 tgctagtgaa aagacaaaag attgtataga acttgcaaaa aaatatgctt gttatgatac
    19261 tcctatgatt attactggag aatctggagt tggtaaagaa ttattttgtc aaagtataca
    19321 taactatagt aagcgtaaaa atgcaccttt tgttgctgta aattgtgctg ccatacctcc
    19381 atctctaata gagagtgagt tttttggtta tgaggaaggt tcatttacag gtgctagaaa
    19441 aaaaggtaaa gcaggtgtat tcgaactagc acatgaaggt acaatcttcc tagacgaaat
    19501 aagtgaaatc cctcttgaac ttcaaggtcg attattgagg gtattgcagg aaaaacaagt
    19561 tatgagaatt ggtggtgata aagttattcc tatagatgta aaaattatct gtgcttcaaa
    19621 taaagactta aaaaatatga tgcgcaatgg tttatttaga caagaccttt attttagaat
    19681 aaatatacta acattatatc ttccttcatt aaaagaaaga aaagaagata ttataaaatt
    19741 atcagaattt tttatacata aatattcaag caaatacaat aaaacgcctt taattatcac
    19801 tccaaatatt aaaaaggcac ttcttgaacg agaatatgaa ggtaatataa gggaattgga
    19861 aggtatgatg gaaagggctg ttattgtgga atcatttgac tcgattttat tagaaaaggt
    19921 tagcaatgaa tatacaaata atatagttga taaagataat agtgattttg aatgcagtaa
    19981 tttagattta aaaactttag aaaaaaaata tatctataaa atttataacg aaaataatag
    20041 aaatactaca aaaacctgtg aaattctaaa gataactcgc tccactctat ggcgtaagtt
    20101 aaaagaaata gaagatgatg tttaaaaata atacatacct atatatatca aaacgtttta
    20161 tgtttaattt taatacataa ggcgtttatt tgttttcaat aaaaaataca attatatgat
    20221 aaaaagaatc tttgtaagtg aaattctcat caatcatacc tttaattata agtaatctac
    20281 agcatttcaa tcgattatta aaaataatca tgataactaa aaatacaaac tctatatatt
    20341 ggcatagtaa ttgcttttat aatacctata taaaaatgaa agggagtgat actagatgca
    20401 catcttaaaa tcagtgaaaa agataccagg aggacttatg gtctgtccct tgttattggg
    20461 agcactattt aatacagtat gtccacaagc tcttgaaatt ggaggattca caacatcctt
    20521 atttaaaact ggtgcaatga gtatacttgc tctattttgt ctatgcaatg gagctcaaat
    20581 caatatcagg caagctggta agccattgac taaaggtata gtacttacag caacaaaatt
    20641 tataattggt gcagtattag gtattattgt tagtaaattt attggaccta aaggtataat
    20701 tggtattaca cctcttgcca tagttgctac catgactaat tcaaatggag gtctatttgc
    20761 tgctcttgca ggtgaatatg gtgactccac agatgttgga gcaatatcta tcttatctct
    20821 aaatgatggt ccttttttta caatgcttgc ttttggagta actggacttg caaatgtacc
    20881 tatagtagca ctatttgcag cacttatacc aatactactt ggatttatac ttggtaacct
    20941 tgatgaagat atacgagaat ttcttgcacc tggtacaaca ttattaattc ctttcttcgc
    21001 ttttccttta ggagcagcat taaactttaa tcaaattata actgctggac tacctggagt
    21061 tgtacttggt ttaggagtta ctataataac aggtatgggt ggctactttg taatgaaact
    21121 tatacgtgca gaacatccag cagtaggtgc agctacaggt acaacagccg gaaatgcagc
    21181 tggtactcct gaagctttag cagctataga ccctactcta gctacaatag cagctgtttc
    21241 aacagtacag gttgctgctg ctattattgt tacagcaata tgttgtccaa tgttagtttc
    21301 ctatctagat aaaagagaaa aaaagaaaaa atctattttt aaaacaaata aaaatattga
    21361 agtaaattaa tacttaagga ggtaaaatta atgaatgcaa ttattcatgc aaatgaaaaa
    21421 gataatctag ttacatgtgt cagaccttta gttaaaggtg aaaaagtttc aattgatgga
    21481 aagtcttaca tagtaaatga taatatacct gtctttcata agatggctac agaagatgta
    21541 aaaaaaggtg atgttgtata caaatacggt gaaattatag gaattgcaac agttgacata
    21601 aaaactggtg actatgttca tgtacataac attgaaagta cacgtggtcg tggagataaa
    21661 aaataggagg aagttaaaat gaaattaatg ggttatttaa gagaaaatgg tcagtttggt
    21721 attcgtaatc atgttttaat tattccaaca tctgtatgtt caagtgagac agctacaaga
    21781 atagcatcac ttgtgcctgg agcaatagca atccctcatc aacatggatg ttgtcaaatt
    21841 ggttctgata tagagcttac agcaaagaca ttaataggat ttggaaaaaa tcctaatgtt
    21901 gccgcagttt tagtagttgg tcttggatgt gatggtattc aagcaaaaga attagcatct
    21961 gaaatagcta caacaggaaa aaaggtagat tacgttgtta tacaagaatg tggtggaaca
    22021 ttaaaaacag tttctaaggg aactgaaata gtaagtaaaa tggctcgtga agtatcaaaa
    22081 gaagtaagag ttgaatttgg tatgagtgaa attacattag cacttgaatg tggtggttct
    22141 gacccaacaa gtggaatagc ttcaaaccct tcaataggcg ttgcatcaaa ccttcttgta
    22201 gatgaaggtg gaagtagcat actaagtgag actacagaag taattggtgc agaacatttg
    22261 ttggctaccc gttttgaaga tgaagaaatg aaagataagt ttcttaaatt tgtaagtgat
    22321 gtagaaaaaa gagcaattgc tatgggagaa gatttacgta gtggtcaacc tactccaggt
    22381 aataaagctg gtggactttc aacaattgaa gaaaaatcac ttgggtgtat gtataaggca
    22441 ggcaataagc cattcaaagg tgctcttgaa tatgctgata ttgtacctcc tgataaaaaa
    22501 ggattatatt ttatggatac tccaggtcaa gatatagatt ctatcactgg tatggtagca
    22561 ggtggtgcac aaattgtaat cttttcaact ggtagaggta ctccaacagg aagccctatt
    22621 tcaccagtta ttaaaatcac tgggaatagt gatacataca acaaaatgcc agacaatatc
    22681 gatataaatg caggtagaat tataacagat ggtgctaaaa tagcagatat aggtcaagaa
    22741 atatttaatg aaataataga agtctgtaat ggtaaacata ccaaagctga aagtcttggt
    22801 catagagaat ttggcattta cagaatttca tctacatttt agattgtaac aaaatatctt
    22861 ttataaatat aatacttatg tagatacata agctaaaagt tctttctaat cctataaaaa
    22921 ttttctatac tgaaaaggaa ctgtatcaaa attattttga aaacagttcc ttttttaacc
    22981 tcttttactc aaaatattat aaaataagat ttaaacaata aacgtgtctt gattaatctc
    23041 taagaagaca taaataaagc taaatgtaaa gtgagataaa taaaatttaa tatctttgta
    23101 tgaaaagata tttggtataa tatgtctgag agaattctta tttgtaaaca aataaaggat
    23161 ggaggatgaa acaaaagaat gaactttgaa aacacaaaaa ataaaaaaat aatagctact
    23221 ttactattga cactcctttt aatcgtgtca ttaacatgtg tcaattttat tatgaatcta
    23281 aataaagtaa cacataatga aacagaacga tatcttgtag aagtttcaca aagtatttcc
    23341 actacaatta atgtgaaatg taaaagtata tttaggacac tatattctat gggagatact
    23401 tattttgaga ttaatgatag tgaaaaagaa gtcaatagat acttatcaaa aaaattaaaa
    23461 ttatttgaat ttgattggtt aggtattata gattctaatg gaaattttgt aagtgccctt
    23521 aaagatacag gtaatgttaa aggacaagac ttttatagaa aagcccttca aggtaaagaa
    23581 acaattactc ctgttaaatc acaatctggt acatatggaa ttttatattc tgtaccacta
    23641 tacaatggcg ataaaattgt tggagctgtt gctgcttgga atactattga tactatgaga
    23701 aatttattaa gtgtagaaag ctttcatggt gaaggatttt cccaaattat cgaccaagat
    23761 ggaaatttta ttgtaagttc agcaaacaaa aatgctccaa aaaatgtggt aaattttttt
    23821 gacatgatta aacaccaagg aaaacaggtt gataaaaata aacttgaaga aatgaaaata
    23881 aacttaaaaa agaacaaaac tggttctatg tattatacat taaatgatgg tatttctaag
    23941 gcaatgcatt atcttccatt acaagaagaa aacttatacc tcctttcagt tgttccaaca
    24001 aaaacagcaa gtgcacagtt tgatttactt ctaaaaaaag caatcttcat caatatttta
    24061 attgttatac tatttttatt gcttattata ttgattatgt ttataaatca aaagaacagg
    24121 gcacaactta taaaaatcgc ctatgtagac tctgtaacta aaggttacag taaagcacgt
    24181 tttgatattg aagctaaaaa aattatacag tcatcttcat cagaaagcta ttcattggta
    24241 tctataaata ttcagaaatt caaacttatt aatgattctt ttggtagtga agcaggagat
    24301 aaaacattaa agtacataca tgatgtcatt agtaattatc tagaaccagg agaattgctt
    24361 tgtcgagttt ctaatgacca gtttaatgta cttattaaaa ctattcctca aaaagaaatt
    24421 cttaagtaca tagaaaatat caccaatgat attaatagtt tcaataaaaa tgaagaatgt
    24481 aaatatttta tagctctatc tataggtgta tataaaattg atgacccaaa tctaccttta
    24541 gtttcaattc aagaccgtgc aaatgttgca cgaaaaaata ataaaggaaa cattggaaat
    24601 catccacttt atacatgtgt tttttatagt gatttagagc gtttaaaaat gattaaagaa
    24661 aaagaaatgg aaaatagaat ggaggaagca ttaaataata acgaatttat agtatatctt
    24721 cagcctaaaa taagcctcaa agataatact attgtaggtg cagaagcatt agtaagatgg
    24781 caagacccaa ataaaggcct tataccacca gacgagttta ttcctttctt tgaaaaaaat
    24841 ggttttatta caaaactcga ccactatgtt tttgaaaaag tatgtagtat gataagaaaa
    24901 tggatagata atggagaaac tccaatgcct gtttcagtta atttttcacg tgctaattta
    24961 aataatatag attttttaga aaaatacaga atgcttcgta ataaatacaa cattcctgct
    25021 aagctcttag aaattgaatt aactgaaaca cttgtatttg aaaatctcca aaaattaatt
    25081 aatataatag accaaatcca tgaagaaggt tttcaatgtt cactagatga ctttggaagt
    25141 ggatactctt cactcaatat gttaaaagaa ataaaagttg acacatataa aacttgaccg
    25201 tgcttttttt agctcaccta atgcagataa tattgctgaa aatcatgtaa tagaatctat
    25261 agttaatttg gcaaaaaaat taaatatgaa tagtatatca gaaggtgttg aaacaatctt
    25321 gcaaatggat tatcttaaaa aaattaattg tgatatgata caaggatatg ttttttcaaa
    25381 acctctgcct caagaaatat ttgagaaaat gacttttgga aaaaaactag aagaaaccag
    25441 tatggatagt tccgtttaaa taaaataaaa aacttgtctc tataaaataa ttttaatcat
    25501 tttataaaga caagtctttt tattttccaa tttaatttta ttttctaaat ttcttatact
    25561 cggcttgttt taaattccct ttttaagaat aataatacga ttatcatagc aataaaatct
    25621 gcaactggct gtgccaacca aacaccatct aaattaaatt gaattggaag tacaatgatt
    25681 aatggtataa ataaaataaa ttgtcttaaa agagttaaaa acattgaatg tttaacttta
    25741 cttatacact gaaaatacac aggtcctaca atagaaatcc ccattatagg caatgtaatt
    25801 agatttatgc ttatacctct tactgctata tccatcaaat ctgaatcttt attaaatata
    25861 cctacaaata cttctgggaa aacttgtact attaaaaatc caaatgacaa tatgacaact
    25921 gacactatga tagataatat taaagttttc tttgcacgtt catattgttt tgcaccataa
    25981 ttgtatgata ttatagtttg cataccttga ttcaatccaa acacaggcat cataaacatc
    26041 aataaaatag atgtaagtgc tgtcatagcc ccaatagcta aatctccacc ataaaccttt
    26101 agagaattgt ttgtaaacat gtgagttata cttatagcta cttccatagc aaaaggtgtc
    26161 attcctattg caaatacctt ctttactata tctttattca attttaagtt acattttttt
    26221 agttttaaag tggacttccc tcttataaaa taatatgtta cccaaaagaa tactactatt
    26281 tgacatagta ctgttgctat agctgctcct tttataccta aattaaacac aaatataaat
    26341 atagggtcca atatcaggtt taatacacat cctacaatca ttgtccttgc tgctaatttt
    26401 ggattacctt ctgctctaat tgcattatta agagaaaatg caactaaatt aaatatactt
    26461 ccaatcaata tgacactcat atagtctttt gcataaaata ttgaatcttt acttgcaccc
    26521 aaaataaaaa gtatatcttc taagaaaact aatcctaata tagttataat taaagagata
    26581 attatagata atgtcaaagc attacctaat atcttctctg cttcttctct atttctttcc
    26641 cctaatttta tagacaatct tgtgctagca cctactgata ccaacattcc aaaagctatt
    26701 attagagtaa atactggcat agttacacca agacctgcaa tagctataga gccaatacct
    26761 tccatagaac caataaaagc tctatctact acattataaa gtgaagttac catcattgct
    26821 attattgccg gtatcgaata cctaaataat aaaatccata ttttttcatg tcttaatgct
    26881 tcttgattct ccatgttgtc accatttaca ccatgtcata agttatacaa attggtttat
    26941 ttgttcttgg gtctgttgct atttctgcat caatattaaa taattctttt agattatcct
    27001 tcgtcataac atcatatgca tcaccttcac aaactatttt acctttctta atacctatca
    27061 tgtggtcagc aaatcttgct gcattattaa gttcatgtat taccatgaca atagttcttc
    27121 cttgtttttt atttagttca tctaataatt ttagtatctc taattgatgt gccaaatcta
    27181 aatatgttgt aggctcatcc agtaataata tatcagtttc ttgtgccaaa gccatagcta
    27241 tccatgccct ttgtctttgt cctcctgata aatcttccat atttctatcc ttaaattctg
    27301 caattccagt aacttctaaa ctccaattta caatctctct gtcttcttta ctagaattac
    27361 caaatccact tttatgagga aatcttccat aagaaattaa ttctgaaacg gtaagaccac
    27421 taggagcttg agggctttgt ggaagtacag ccatatcctt tgctatttct ttagacttct
    27481 ttttgtagat gtcctctcca tttatatata ttttcccact attagggcta ataattctac
    27541 ctatagtttt aagtattgtc gattttccac aaccattagc ccctatgatg gtagttattt
    27601 ttccttttgg tatatttaaa tttaaatttt ttataatatc tgtgtttcca tatgatacat
    27661 ttagattctt agtatttata cagttcataa caacaccctt tcctaatcat ttaccaacat
    27721 taaataaata aagtacggca ctcctattat agaaactact atacctactg gtatttctat
    27781 aggtgctaga agatttcttg atatagtatc tgctattaaa agtaataatg ttcctatcaa
    27841 tgctgatact ggtattaatc ttttatgctt tggtcctatt atttttctac ctatatgtgg
    27901 agctattaaa cctaaaaatg aaatactacc tgctactgct gtcgatacac cagataaaat
    27961 tactgcatat attataagtc ttcttctttg tttttctaca tctacaccaa gacttataga
    28021 tacttcatca cccaaattta aaacatctat atatttagat ttataaatag ttatacccat
    28081 aaaaatcact ataaatggaa gcacagctat tacatatttc caactggttc cccaaatgct
    28141 tcctgatgtc catgccataa ccctattaaa ttcttgtgtt gtaaatttca attggaatat
    28201 cgtcaaaatt gaagtaaaag ctatgtttac acctatacca ataagtagta atcttgacga
    28261 atttatacca cctttccatg caagagaata tataagaaat gctccaaata aagctcctat
    28321 aagtgcaaca ataggcattg taaatacagt caagttactc ataccttcat atacattacc
    28381 attcataagg tatatataaa ctactacaaa caatgcagca cctgaattta ttcccaaaat
    28441 tccagaatca gctaaatcat tctttgtaac tccttgaagt attacacctg ctgttgataa
    28501 tgcagaacca accaaaagtg ctattactat tcttggtaat cttaatttaa atattgcaat
    28561 ttcatgatta cgctgaccct gccctaaaaa agtctttata acatccattg gttctataga
    28621 aaatgagccc atatttaaac tcaataaaaa agtgataaaa ataagcacca ttaaaattga
    28681 tataacaagt aatgttttag aatttatttt tataatattt ttcatagtac ctatttctcc
    28741 ccctttctaa caaggtatat aaatattggc actcctataa gtgctgttat agaacctact
    28801 ggtgtctcat atggaggatt tatcatccta cttaatatat ctgtatatac aagcaataca
    28861 gctcctaata ctaaagagct cggtattata tacttataat ctgaacctac tattccttta
    28921 gctatttgag gaactataag acctataaaa ataatattac cagacactga cacagatgct
    28981 ccagtcatta atattacaag tactatacat ataaatctaa caaagttagt tttttgtcct
    29041 aatcctattg ccacttcttc tccaagactt aatatagtta ttcttggagc cataataata
    29101 gctaaaataa atccaatacc accagctaca aatagcaatt ttataccttc ccacttcact
    29161 cctacaagcc ctcctgatat ccagaaactt aattgttgag ataggttaaa atacatagat
    29221 attgctgatg ccaaagaaat tagcaatgta ccaagagcga ctcctgcaag agctaatttt
    29281 acattattaa ctcttcctct tgatttagaa cttataaaat atataaataa tccacttata
    29341 cttgctccaa taaatgcaaa catcattaca ctaaaacttc catgtataag ttgaggaagt
    29401 tttttttgaa gtaccagtga tatcgaaatt gcgaatgtag ctccttgtgt aattcctata
    29461 acagatggtt cagctatagg atttctcgtt attccttgca ttattgcccc agaaactgct
    29521 aaaaaccctc caacaagtgc agctgctatt gctcttggaa gtctaacatc ctttactatt
    29581 ctcatattta ttccatcttc catttttatc aaactatcta tgacagttga tagattcata
    29641 tcttttgccc caatagttat ggataataca attccaaata caagtacaac tatacttact
    29701 aatatcaaca aataagttat atttttttta ctcagcttag tcaattatcc tacctccttt
    29761 tcccttttaa tcgtcagtat aatatcatct attaagtatt ttaattttaa gatttattaa
    29821 aaataggtgt gaaaaatgaa tatcattatc tcacacctac taagttaaga attactttgt
    29881 taattcattt ttaactgatt ctaataataa ctcttttcca attggattgt atgattgact
    29941 gaaaaatgga gcagcatcaa gtattgttac attaccatct tttactgctc ttatttgaga
    30001 ccagatagca ctattttcta aatctttttt atctgcttct gtagcaatta caactatatg
    30061 gtctgcatct atatctgtta aaccttccat acttaccatt ggtagtgtta ttccatcctg
    30121 ttttggcata ttcttaggtc tagctaattt catatcatca ttgattagag tacctattcc
    30181 accatcacta aataccataa attgacctga acttgccaac actggtaaat aagttttttc
    30241 tccattcttt tctataactt ccttacctaa ctttgtagct ttttcatcat acttctgtag
    30301 ccaactttta gcttcttctt ctttgtcaaa taactttgaa acatcagtta acttgcttct
    30361 ccagtcattt gcataatctt tcatcataac tacaggagct atttctttta attggtcata
    30421 tattttttct tgtctttgac tcattattat taaatcagga tttacttcta atatagcttc
    30481 catatccata gtatccatca ttgagtgccc aactatcttt acacctttaa gctcttctct
    30541 tatgtaactt ggtaatttat cagtttcata tgagtctaca tttgctgttg caacaggctt
    30601 ataacctgct aataatagtt cttcactact tcctgatata tcaactattt ttttaggatt
    30661 tgatggtatc ttaacctctc ctttaacaga ttgtacaact cttgtctctg atttatcttt
    30721 atcctctgta ttcttatctg aacatgctgt aacaagtact aacgtagtta taatagatat
    30781 aaatattgca agtgatttta tttttttcat gattacttct cctacttgtt tattatactt
    30841 acatgatatt gattatcata agcacatatt tattatagca tttttctatt tttagtcaat
    30901 attattttgt aaaaggataa aaattcctaa ttaatcttaa ttatatatag cttagataag
    30961 gttttcttat attaaacagt atttttttta agaattggac acaaacacaa tgacttttag
    31021 gttaaacaat gataaaatgc aatttataga taatgaattt agtaaggagg attcaaaaca
    31081 tggtacattt agtatattgt gataatgcag gaaaaaaagg agaaaaggtc ttagataaaa
    31141 tcttggctgg aacaaaaaca atggttatta gaggtgctgc tggtagaaaa ataccacata
    31201 gtagagtatt tgaagatgag ttactttatt ttatggaaaa aggaactgga aaaatatctg
    31261 caaaagccac tgttaaaaaa gtagaaaact atgttaaatt aacagaagat gaaatttcta
    31321 aaattttaaa tgaaaatcaa ccaaaattaa atttatctga aaagcaaaaa gttcgttggc
    31381 ataaaaaatg tttatgctta gttgaatttg aaaatgttga agaaataaca cctcttgatt
    31441 ttgaccatca aggaaatatg gatgattggc ttattgttga aaaaattgaa gatgtagttg
    31501 ttggtactag tataccttac aattatgaga agtctaaatt ttaaagtatc aataacataa
    31561 tactatattg ctaaactcta cttaaataaa aaaagagtgc atttaataat tataaaaatt
    31621 atttttatac actctttttt aataataaat ttaaattact tactttctaa tctttcatat
    31681 acatctataa attcttctgc taattgttga aaattcatag ctgtcattaa atggtcttgt
    31741 gcatgaataa gtataacact tatttcagtt ttattaccat tagcttcatt ttgtatcaat
    31801 tctgtttgga atttatgagc tttatgaatt gattctttcg acttttcaat acattctcta
    31861 gctttatcta tattcccgct ctttgcttct tttattgctt caaatgctag ccctttcgca
    31921 tcacctgcaa atccaattat tccaaaagat atctctatca tcttctcatc catttattcg
    31981 acctcctaaa tatctctata gcttgatatg actaatccat tcttttctaa aaattctttg
    32041 acttttttag atgtaagtat cttgtgctct ttcgttctat caatagcata agatgtagaa
    32101 tttaaaatat aatcatctaa aaaagctggg tgagacataa tatccactac atcatacttc
    32161 attatttcgt ctatattttt tataaaatat tcttcactta cattttcctt ataaaaacta
    32221 tctattaaag gaactacttt agaatactct aaattatatt caaatccacc tcttattgga
    32281 agattatact tggttacaat cttctctact actggcttta aacttactaa agtatggata
    32341 tgatggtgac tatctaaatg agatatatct attccaagcc cttttaccct atctatttga
    32401 gcacaaagtt ctctataaat ctcatcacaa tccatttttt caataatttc gtctgtaatc
    32461 ctcctaataa aaaaaccgtc tttatcaaca atagttttta agttagataa taatggtcta
    32521 ccacaactta aagtcatatg aactccacaa tttaaagttt tattttcttt aagtaagcct
    32581 actccatgtt ctaccccagg catatttgcc atcatagaag tagacctaac tataccatta
    32641 ttatgagctg aaattattcc atagttcaca gcttcacaat atccaaaatc atctgcattt
    32701 attataattt tagtcatacc ataacctact ttctatagac aactatctat taaatctagg
    32761 aagataatct ttatgagctt ccaacatttc gtctaatata gtttttgcta agtcatcaga
    32821 tggaatcaat gggtttatac aaagtgccaa aagagcagat tcataatcgc catatacagc
    32881 tgctttagct accaatactt caaatgattt gatttggctt actaatccat gcacagaatc
    32941 tggtaaatat cctatactta ttggttttgg accatttttg gttattacac tactcacttc
    33001 taccgcttgt tcatctttaa aatctcttat agaaccattg tttaatgtat taactacttg
    33061 gatatctttt ttgtcattgt atatagaact tattaaatta caagcagcat cactatagta
    33121 agcccctcct cttttttcaa gttgaggtgg ttttacatct aatttttcat ccttatataa
    33181 ttcaaataat tgttcttcca attctttaac tacttgacct ctagcttttc cctctttaaa
    33241 ctctctcaac tcatcctcaa gcatttcttt agttttatag tagtatctat gatatggaca
    33301 aggtattgca cccaaagatt ttacaaattc agcattaaaa tcaattgcct ttatattttg
    33361 catacttata tctgctttga caaacttatc aattgcttct tttgtaacat cttctccatc
    33421 taaagataca tttagaccat aaaccatatg attaagacct gcaaaatcca tacttattct
    33481 gtcactttct acattaaata gtttggcaac atcatttttt aaatgaattg gcacattgca
    33541 tagacctata tatcttctaa aattagtata tttgaataca gcctctgtta ttattcctgt
    33601 tggatttgta aaattcacca tccatgcatt tggacataat tcttccacat cttttattat
    33661 atcaaatata actggtattg tgcgaagggc tttaaataaa cctccagcac cattagtttc
    33721 ctgccccata actccatgag ataaaggaat agtttcatct tttattctcg catctaaaag
    33781 acctactcta agttgtgttg tcacaaagtc tgcatctctt agagcttctc ttctatctaa
    33841 agtaagattt attttcatat ctactccagc tttttttacc atcctcttag ctagatttcc
    33901 aactatctct aatttttctt ttccagcttc tatatctaca agccataatt cctttacagg
    33961 taactcttca tatctattta taaaaccctc tacaagctca ggagtataac ttgaacctcc
    34021 tccaattgta actattttta gtcccttttt catctcatta taccatcctt acctataatt
    34081 taatatactc tacaaaaccc tatacattta tttaccgaaa taaaactacc tagttgttaa
    34141 ttttcaattt attagcttcc attttttcag acgctatgac aaatggaata tatattaata
    34201 ttgataatat taaattaaat agtgctaaag ctccaccagt ccagtcacct gtagccatcg
    34261 ctccacctaa tataggaggt gttacccaag gtattttagg taaaattact ggtgctacta
    34321 atccaaaatc tattgctaag tatgatacag cactacatat cactggtgca agtacaaatg
    34381 gaatcataaa tataggattt aaaactattg gaagtccgaa tatcaatggt tctcctatat
    34441 taaatattgc aggtggccca cctaatacta tcatttcttt acgtcttctc ccagctatta
    34501 ataaagctac tattaatccc aataccattc cagaaccacc taaatataca aatgcatcaa
    34561 agaatgcacc tgcaagtaca tggtatcctt ccgttgctcc tgcttgagcc aaggcagcat
    34621 tttctccacc taatttcata agtatagttt ctgtaaaagg aagtgctata tttgcaccat
    34681 gaagtccaac agtccaaagt atatgaatta taaatgcaat aagcattgta gaacctagac
    34741 tatcagctac atttgatagc ggtgctccta aataagtatt taaaatatct gttaagaaac
    34801 ttccattaga aagcattttt ataaatattc ctattacagt aaatatcatt atagttaaca
    34861 ttcctggtat taactttgca aatgatcttg aaactgctgg tggaactcca tctggcattt
    34921 ttatagttaa tttctttacc ttagataatc ttacaaatat ctcagttgat aataatccta
    34981 ttaatataga agagaataaa gctgctgtac ctaagtaggt ttgttgtatc attccccatc
    35041 cttcaactac tgtagttcct ccctcaggaa ctattttacc aatttgtgga gccattataa
    35101 aaaagataga taaagctata agcccagatt gtaacccatc actctcataa gattttgcta
    35161 gaaaataagc aacacctatt actaagaata ctgccattgt acctattgct ccccaccaaa
    35221 ggtcgcctcc aaatgtggtc caattttctc caaatatact tgccatcaaa tttttgtatg
    35281 cttccaatgg taagttattt atcaatgttc ccaaggcacc taccatagtt ataggtatca
    35341 ttactataaa tgcatctctt actgctacta agtgcctttg agctcctatt ttagcagcta
    35401 caggcactat atatttatcc ataaatgaca tgaatttttc cattttacat cctccagtac
    35461 aactttattt tgaactattt aactctacag ctctatctaa cacagctttt ccattacatc
    35521 ttccataatc catcatattt ataacttcta ctggaatatt atatggtttt gccatttcag
    35581 ttgcttttcc taatacatat ctaacttgag gccctaaaag taagacatca caattttcta
    35641 tttcattttt taagttcact tctgatatag cccatatttt tgcttctata ccgttttcct
    35701 ttgctgcatc ctccattttt gtaactaata aactagttga cattcctgct gaacaagcta
    35761 acattatttt tatcattttt tcccctccaa attagattca ttaaatattt tttaagtcat
    35821 aaatttttta aactatacat ttaaaatatg cttttctatt aattttttct taatgtatat
    35881 atcttatttt taaatgtata tacatttaat tcttgatatt attatatacc aaatgtatat
    35941 acatttcaag aaaacttttt cattttattt aaattttatt cgacaacagc attcaaagtt
    36001 aatgtatatc catagtgata tgtgtgtgag tactcaaaaa tctcactatt ttgtaactta
    36061 caaatttggc ttatatataa aagtggctct ggttcttcta attttagatt atccatacat
    36121 tcttcattcg atattacagc ttgtacttct ctctctgaca tggctatctt gtatccacac
    36181 acttcttcaa caaaattata aagagaattt tcacaatgag tcttatttaa atttggaaat
    36241 aacttaactg gcatataagt aatcatatat gaaactcttg catcattggc taatcttact
    36301 ctttttattt cccaaacatt ttcttctaaa cttatattca acttacttga taaattttca
    36361 tctgcctgaa ctatttcaag agataccaat atattagata ctttatagcc tttactttcc
    36421 atctctttag taaatccact tacagacgat atattatcat atatattatt tgcaataact
    36481 atactaccta cacctctttg tctagttata tagccttctt taactagatt attaagtgca
    36541 gaccttacag tcattctagt tacattaaat tcttcactca gctgtttttc agaagggatt
    36601 aaatcccctt ctttcaaact gtctgaattt attaactctc taacatgatt ttctataact
    36661 ttataaattg gctctttcat ttaattcact ccttattaat taaatctttt atataataag
    36721 ataattttat atacaaataa agaaaatgtc tatacattta agataattta aattttattc
    36781 agacatcttg tacaactttc cgagttttaa taaagatata aatagactta tcattaatat
    36841 accttttaat atacttgata aggttatact ccaccataca ccatttagac ccaacatttc
    36901 tggtctagat ataaagtaag ccaatggtac tcttgcacca gtcagtatag taattataat
    36961 agaaggtata taggttctgc ctaatccttt gaaagctcct gttgttatta tttctaaaca
    37021 cataaaaaat tgagaatagc ctaatatttt aagataaatt gctccttttt ctattgcctc
    37081 actttcatct ataaaaatag aaaatatact ctctcctgca aatactaata ccaaagtagt
    37141 tattatccca agtacagaag atacagttat agctattttg caacctttat ttattctaga
    37201 atacttcttg gcaccataat tttgtccaac aaaactgcct aatgcaactg atagcccttc
    37261 agccgtcatc caggaaattg cttctatttg tgagcctact ttttgtactg caacagccac
    37321 aggcccccat gatgctacta ttactcctaa tagcattgag aatactgtaa acataccact
    37381 ttgtattgct ataggtaatc ccaatttata cagaacttta taatatttag tctctatttc
    37441 tttaaatagt cttaatttaa aatattcttc tttactttta actattatat atataaaaca
    37501 tgtagtaact actatctgag caaatacagt tgcaatagca gctcctgcaa caccaagttt
    37561 tggagcaaat ccccatccaa aaattaatat aggatcaagt ataatattag ttattaatcc
    37621 tactgtatta atacgaaaag gagttttact atttccaaga ccgttgaaaa ttgcagtaaa
    37681 tactggatta ataaaataaa ataccatacc tagagctact attatcaaat actgtctaga
    37741 catggttata acttctaagt ctcctaatct aaaaaaacct atcaattgtt tattaagtac
    37801 tattaaagaa attgtatata ttattgctag tataatattt atttctattg ctgacttcat
    37861 atatgattta gtagtgctta tattatttgc accaatactt tgtgctactt ttatctctcc
    37921 acctactttt gaaatcataa taaatgccat tgcaagccat ggaaaaaaac cagctgttcc
    37981 aactgcagct actgctttac ttccggcttt accaacccat atcatatcta tcatgttata
    38041 ccccatctgt ataaatgaag tccccattat tggaagtgat agcttaagta atttttctgt
    38101 aattcttcct tctgttaaat taattctgtc tttcattttc cacctctttt atattaaatt
    38161 tatttgctta tatagctaga ttaatcattt tatttgattg agttcactat ccttcaattt
    38221 atattatcat atctcaagat tgcatctcaa ccatacttat gtttaagata aagtatttta
    38281 cctatctctg ataaaattaa tacttatgaa tataaatata gtcatttcat caatacatat
    38341 tataaccctt aacttaactt caattaagta tacatactgt aatcaattag aatattttat
    38401 aatgtatctt ccattaagaa ataacaaata taagtgattt atctataatt ttatttatac
    38461 ataaaatagt atactgcaat aataaaaaaa ctatatttga atactttaag catgtttact
    38521 aattgtaatt tccacacaaa tttgataata tatgaatata ttatggtgaa atttatagat
    38581 atatattaat tatggggaga ttgtgggagg ttcttttcac aagacattta agctatgaaa
    38641 ataaataaaa aatttaatag agttgatatg caggtttcaa tacttactgc aattattgta
    38701 ataatatctt cctcatgtgt atattttttt aattatacat taacttataa gagcatgata
    38761 tactctttaa gtgagaggtc aaaacatatc tatgaatatg tagagagaaa tattgataaa
    38821 aataccttta ctgaaattaa ttctaaagaa gaccaatcaa aaaagtctta taaaagttct
    38881 aaaatcctat tagaatctgt aaaaaatact actggagtca tgtatctata tactgctaaa
    38941 aaaacaaaag atggatcact agtatatgtt gttgatggtt taaattcatc tagaagtgat
    39001 tttagaaatg ctggtgattt gatagaaaaa gagatttggg gagatcttaa caaagcatta
    39061 gataatcata tattgttacc taaaaagatt aaatcaacat cttggggata catatttata
    39121 tcgtattttc ctattcacaa tgatgttggt aaggttgttg gagtaatagg aattgaattt
    39181 gaagcaaaac atcaatacaa tacatttaaa tttataagta tagttacacc aataatagca
    39241 ttattaactt gtctaatatc agcattgatt gcagtgattc tatttaaaag aatatctaat
    39301 cctacatata ctgattttgc taatacagat tatttaacag gcctaaaaaa tagaaatgct
    39361 tttgaaatag atatgaataa cttaaacaat tcaaatctaa agaatctaat atctatcata
    39421 tcaatagatt tagacggttt aaaaaagatt aatgataaat ttggccatca gacaggagac
    39481 ttatatatta agcaagcaag taaaattata caagatgtaa taaataaaaa atatatagtt
    39541 tatcgtattg gaggtgatga atacataatt attctaaaaa atttat
//
LOCUS       DAMJWP010000011        36766 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.011, whole genome shotgun sequence.
ACCESSION   DAMJWP010000011 DAMJWP010000000
VERSION     DAMJWP010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 36766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36766
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.011"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(<1..1800)
                     /locus_tag="PC700_000536"
     CDS             complement(<1..1800)
                     /locus_tag="PC700_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2579860.1"
                     /translation="MKKYEIFIFMSIIVLCFISFPHKVFGQEENCLEDFERWVQENKN
                     NKEEIVYTLSCNMVIDEEFRFYIPYDSNFTIDTNKYKILIKNHGRFIIDDNELNIIGE
                     GGKEGVIHIENGGSISIGINNIIATDGTALYVEEGGDLHIKSSYESVEKIKANGKDAI
                     GIYTENDIRILNKDIEVNGEHAIGVYSKGDVEIEETSIKAYSSNNESGLLRDNNKLAQ
                     SIISESKKVYIIDDYNELIPSIAKDSGYNIVKCCHRGIGVFSDEIKVSKDDKIENIKF
                     PKYMVLETSSGNQLDIDVEWDFTDYYEKLEKGENFDITGKFKTEMLNKEKIIINDDVV
                     PILNVSVIEKKTIDNLELEFQNTKNGYVAVLFYDMPYSASKVFVEYSSDGINWISEEQ
                     EDIRDQAILFFEDFKLRCFRVKVVGGLKEGYSNIVFKPGFIMGGGDNQETPDDEREND
                     DIDGDRGGGGRDDPDRDEDDNNQDGNNGNENNDSNNNQNNNNENNNSDEKQPNTPKDD
                     VESSTDSNENKNPHDIPKDGNKENNSDKEKGFYNKNPITENDDNYDLSSKSVFGTNEH
                     SFPYRESISNKNQDTNKNKSNISDYDGIINNNYK"
     gene            complement(1984..3459)
                     /gene="spoIVA"
                     /locus_tag="PC700_000537"
     CDS             complement(1984..3459)
                     /gene="spoIVA"
                     /locus_tag="PC700_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HDF2579861.1"
                     /translation="MNNNIYEDISKRTQGDIYIGVVGPVRTGKSTFIRKFMEKLVIPN
                     IDNEFKKDRTRDEIPQSGSGKTIMTVEPKFVPADGVEIKIKDTVSLKVRMVDCVGYIV
                     EGALGHEEGGKQRLVSTPWSQEAMTFEKAAEIGTKKVIKDHSTIGIVVLTDGSVTGID
                     RKSYVEPEERVIQELKNLKKPFAVVLNTLSPKSEETSMLRSELEEKYEVPVLPMNVVE
                     MEEEDIEEVMEAVLYDFPLTEIRINLPKWVEGLERNHWIKSSIITTLKQSIIDIGKIR
                     DIEGIIQGFSELEFLEDTGVDNVELGEGVINIDLQTKQDLFYNVLEEKSGFKIEGDYQ
                     LLSLITRLSKVKNEYDKIESALIDAKIKGYGVVAPSLEELSLEEPEIMKQGKQYGIKL
                     KANAPSLHIIKADISTEVSPIVGNQNQGEEMIKYLMEVFEEQPADLWESNMFGKSLHD
                     LVKEQLQSKLYTMPEEIRVKMQKTLQKIVNEGSSNIITILL"
     gene            complement(3631..4926)
                     /locus_tag="PC700_000538"
     CDS             complement(3631..4926)
                     /locus_tag="PC700_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2579862.1"
                     /translation="MSVEVNKPSIWKNFKVLIILFVAGAFIYALPYLKNYYYDTFAQA
                     FNLTNTQMGSLGSIYGLLAMVSYLFGGFLADRISARKLLSGSLIITGISGLVLTLYPP
                     YIVVFLIHALWGITTILTFWPALVKAVRMLASENEQGKAFGFMESGRGITNAIHLSLV
                     LILFGYISSKVSDKSGLTAIIVLYSAINVIVGVLVFFKLKDNEKEVSAKFDKEAFFAV
                     IKMPHTWIITIIMFCSYSANMSFYYFTPYSTEAFGATVVFASAISILAQYCRPVASAA
                     SGLLGDKVGSSKIISYGFILMIAGLLGVIFTPTKSSMIYILLIGCVAIYVSMYAIQGL
                     HYSLLEEGNYSLSISGTAIGIVATLGYLPEVLCPLAAGKILDAFPGVTGYRYYFIVLT
                     IVAIIGLVFTFIWMNMTKERRKELIEMNNKSNENTTKVS"
     gene            complement(5110..6519)
                     /locus_tag="PC700_000539"
     CDS             complement(5110..6519)
                     /locus_tag="PC700_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2579863.1"
                     /translation="MKKKIGFWDLVFMNISALFGIRWIAKSTASSFGLGLGSIPVWLL
                     FAFIFFVPASLICAELAATYPKDGGLGEWVKQAYGEKWGFMVSWLNWTSKIFWYSSFL
                     TFLAINIAYMLGNPDLSNNKMFILALSLIIFWLLSLVSTRGMAFGKFFTNTGALGSTI
                     PAILLIVMAFMSVVILKKAPSASIYTIQNIIPKIDANSLVSISAIIFALSGAETTANF
                     ITEMDNAKKNFPKAILTVAVLIGGIYILGSVAITMILPPDKIAASTGILDALAKVAQD
                     LGIGSWFIRIVAFGITLSVLGALILYIAGPVKMLFGNVREGVFPKQLTVTNKYNIPSN
                     AVIVQAIIVSLLLVGTNLLPSVDNIYNVLVTMTALTALFPYVLLFTSYIRLRKTRPNE
                     ERPYSIAKSDSVCINIARMVLVVTVVGILFSTAPVMETLKDNIIYEIEMIGGGLFVII
                     TGLMLWKNYEKKISNNSGR"
     gene            complement(6571..7710)
                     /locus_tag="PC700_000540"
     CDS             complement(6571..7710)
                     /locus_tag="PC700_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2579864.1"
                     /translation="MSNNMRLERLRKYMNEKSIEIALIFEPDNQFYISGFKAITYSRP
                     IVTVVTQDKIELIVPGLEELHAKEVAKVDNVYVYYEIPEMKEHGISHKHYLDIILNKY
                     PKGTTVGIEKDIVSASFSEYITDKSFAIKDVGSKIFEMRYVKDAKEIEFLKIAGYLSD
                     IGIKGSLENVRVGMSELEFDVAGDNALLKYVSENYPDTYIGFANWTCSGIDRTAQPHL
                     DSNTRILQRGDIVIHSRQVWYENYRAENERTFIIGKPTERQKEVFKIAVEAQQAGLDT
                     IKAGIPARMVDEAARAVVAKYGLELYSNHRIGHGLGLSEHEEPYLRFDNELILEEGMV
                     FSMEPGIYIPGVGGFRHSDTAIVGKNGATIITNYPRSVEELILDI"
     gene            8029..8679
                     /locus_tag="PC700_000541"
     CDS             8029..8679
                     /locus_tag="PC700_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2579865.1"
                     /translation="MTNKELQKKRILMYFIEAAQNIMENEGIENITLRKVADMAGYNS
                     STLYKYFKNLDHLISFASIKYFKDYNLNISRCIENVNDEYKKYIIMWKLFCKHSFDNA
                     QAFYQVFFNLSSDELSYITKQYYDMFPEDLGIHDSDISLMITGESIKERNKILLNNLV
                     DTGYIPAKDLDIINDIIISFYQNLLFLKKNTGKNSGDDELTMKMISSIEYIIKHAK"
     gene            complement(8836..9855)
                     /locus_tag="PC700_000542"
     CDS             complement(8836..9855)
                     /locus_tag="PC700_000542"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDF2579866.1"
                     /translation="MEKVCVLGTGSWGSALALGLAKKGNDVSMWTRKEEQAKKINRTK
                     ENTDYLPGVLFPNNITISTDIEKTIKDCKVIVLAVPSQAVRSTCQKIKPFIKEGQVIV
                     NVAKGLEKGTGLRLSQVCEEELPQNPYVILSGPSHAEEVARDIPTTVVVASKDLKIAQ
                     MIQDLFMSPKLRVYTNPDIVGVELGGALKNIIAFGAGICDGLGYGDNAKAALMTRGIS
                     EMSRLGIAMGANMSTFAGLSGIGDLIVTCTSMHSRNRRAGILIGKGMSLEDTLKEVKM
                     VVEGITATEVAHDVAEKLDIDMPITNAIYSVINKGSNPKEVGIELMMRSKKHEMEEVV
                     LGDDI"
     gene            complement(9886..10500)
                     /gene="plsY"
                     /locus_tag="PC700_000543"
     CDS             complement(9886..10500)
                     /gene="plsY"
                     /locus_tag="PC700_000543"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDF2579867.1"
                     /translation="MEIFSYIIIAVVAYLLGNISTSYIVAKRIAGVDIRTQGSGNAGS
                     TNVLRTLGKRAGAMTFLGDVLKGVMAVLISEFAARLVGIDTLLAGYLAVICVVAGHNW
                     PAVLGFRGGKGVATSLGAMLAVNPVITLMCLAVFILVVAITKYVSLGSVVGIGCSPIF
                     MIMVKNKAGLIVALFLTASVIYNHRANIKRLLNGTERKIGQKKE"
     gene            complement(10535..11854)
                     /gene="der"
                     /locus_tag="PC700_000544"
     CDS             complement(10535..11854)
                     /gene="der"
                     /locus_tag="PC700_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDF2579868.1"
                     /translation="MSRPVVAVVGRPNVGKSTIFNKFAGKRISIVENTPGVTRDRIFA
                     EVEWLDKYFTLVDTGGIEPDSEDIILSQMRNQAMLAMDMSHVILFIVDGKAGITAADK
                     EIAQLLRKTKKPVILVVNKIDSQSQFDNIYDFYELGFGTPFAVSGANSMGFGDLLDEI
                     VENFPAGLDTEYEEDIIRVAITGKPNAGKSSILNKILGEERVIVSPIAGTTRDAIDTY
                     FEKNGQKFLLIDTAGLRRKSKIYETIEKYSVIRAMSAVDRADVVLIVIDALEGVTEQD
                     TKVAGIAHDEGKGCIFVINKWDLIEKDNKTMSNYTKDIKEKFPFMMYAPIVFVSAKTN
                     QRMNKILDTVEYVSNEHSKRISTSALNEVIGEAVMLNQPPSDKGRRLKIYYGTQTDIR
                     PPKITLFINDKDLTHFSYQRYLENKIRENFGFEGTSIKFEYRQKNKK"
     gene            complement(11888..13216)
                     /locus_tag="PC700_000545"
     CDS             complement(11888..13216)
                     /locus_tag="PC700_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HDF2579869.1"
                     /translation="MEVNVKKVNNIISKVYKDSIAEEIGIEVGDLLISVNEQPIHDII
                     EYRFLLSDEYLDVEIQKKDGEVYIYEIEKDYDEDLGVEFTNPIIDQAKSCRNKCMFCF
                     IDQLPEGMRETLYFKDDDSRLSFLQGNFVTLTNMSEEDINNIIKYRISPINISVHTTN
                     PELRQKMISNKFAGKLYGIMKRLADAHIEMNCQIVLCPGINDGKELDRTIKELAQLYP
                     YVNSVAIVPVGITKHRENLVELNIFNDKSASKTIEQIHQIQQKYLEKLGTRFAFLSDE
                     FYILSNSELPGYEEYEGFLQFEDGVGMIRKLKTEIEEYLNILPENILKREKKVSIATG
                     HSAYEFIQSMADAMMDKFKNLQVNVYEIKNKFFGETITVSGLLTAKDLKEQLEDKELG
                     EALYITRSMLKADEEIFLDNIELNQLEELMRIKIIPCLNEGKDFVDKILK"
     gene            complement(13454..13654)
                     /locus_tag="PC700_000546"
     CDS             complement(13454..13654)
                     /locus_tag="PC700_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2579870.1"
                     /translation="MGMDIGLNEYTLAIVTLDKNMKNGGGCPIFYANDEKDLQNKALL
                     MSKCVGGMVHDMTNGTLIIVKH"
     gene            complement(13656..14588)
                     /locus_tag="PC700_000547"
     CDS             complement(13656..14588)
                     /locus_tag="PC700_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HDF2579871.1"
                     /translation="MDRVFFVAVIIGITSRIIMLNLDQKQYPTQPNVLLSQLILAFVA
                     SSLGALLVPALINRSYTSITFLSLAAEQFRQVRANRRNTLQNLEEVQLVKRGAAFIEE
                     IARTYEVRNYMCIVTSFLTIGLYYVILAETKISEGMSLVISSICGLVLAFILKKLLTR
                     KSIGDIADVMPAEISFVDESILKVGDLKGITNIGLKSDREVYINRGVGIQIIPKNKDY
                     ANAGILFDSGQRQAIVYNIYSRLGLYGDYGEPSFVPLPRRNKEDESIMIAYLPVDKNM
                     EKVMEAVKSCPILSSSKGKNLSLKNYKIGKKGSA"
     gene            complement(14633..15565)
                     /locus_tag="PC700_000548"
     CDS             complement(14633..15565)
                     /locus_tag="PC700_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HDF2579872.1"
                     /translation="MEESLLNANLFRHSFIVALVVGILCRGLVLRVTDKQYPSRPQDY
                     LEQIIISGLSASLGAIALPALIDKEFAALTFFAVAIQQFQGLAEQERITLKNIDNEEL
                     VPKGDAYVEEIASTYESRSYISLFSALVSSIVYIVFARKYDLSFFHCTILAIVSGAIV
                     GLIFRRFLRRNSIADIADIVPAKISFEGPILMVNDVIITNIGLESTREKYRNECLAIE
                     VIPRDLGAFGIVNDIGQRQAIIHNLFIHMGIDRDVDEVDIVAISKTNLEKSTVVIPYM
                     PILKDIDVMIDVVKSTPIIETSKGKQSDFSRKTL"
     gene            complement(15620..16909)
                     /locus_tag="PC700_000549"
     CDS             complement(15620..16909)
                     /locus_tag="PC700_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2579873.1"
                     /translation="MEDLNSIYFFIVMLSIAIVILAVRYTITLRKYLKEFIYVSKKVS
                     NNEYHARLNISAKGELGELARNFNHMIKNMDKTLEEVQYKHLQLRSILKSISHGILAI
                     DVNGNILLINDEAKEMIKSEPNQLVEGHNINVVIKEEEILKQILQFIGSKDNETANIT
                     TKDDIVYKIKVDPVYLQNTNNVIIGSIVNIEDITKKVKLENMRSDFVANVSHELKTPL
                     TSISGFVETLKSNENIDVNTRNRFLEIIESESDRLKRLIDDILLLSFVENKDTLLMEN
                     VVVYDVFKEVYEITQHSASEKNIEVLYKCDDKSISILSNKDYIKQVFLNLVDNAIKYT
                     QDNGIVQVVVLKDEEKIVIKVIDNGVGIPKEDVERIFERFYRVDKARSRDVGGTGLGL
                     AITKHIVKSLNGCIKVKSELGIGSEFIVTILQKNFLK"
     gene            complement(16938..17630)
                     /locus_tag="PC700_000550"
     CDS             complement(16938..17630)
                     /locus_tag="PC700_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2579874.1"
                     /translation="MNTKVLVIDDEMHIVELLKFNLEVSNYEVSYSYDGFDGFIKAKE
                     IKPDLILLDWMLPNISGIEVLRKIRSDKDLKNIPVIMLTAKNMENDKVEGLEIGADDY
                     ITKPFSIKELLARISSVLRRYNLTSLGEENNILTTGNLKLDLSKHEVTKGSEKIELTL
                     KEFELLKLLIQNKGKVLSRNYLLDKIWGYEYYGETRTVDVHIRYLRKKIEDEDKSEKY
                     IETIRGVGYKID"
     gene            complement(17645..18406)
                     /gene="pgeF"
                     /locus_tag="PC700_000551"
     CDS             complement(17645..18406)
                     /gene="pgeF"
                     /locus_tag="PC700_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HDF2579875.1"
                     /translation="MMKDYINIKNVQDIKEYVNLSGNCEELDEKLNSVNLVITCKNID
                     AKNREDMLKVCKECDLTFENITSNSQIHSDIVNIVNKDTIGKRRDGDALITNLEKVPL
                     LLFTADCVPISIIDAKNKAIGLAHAGWRGTFSNIGHKTIKLMSECYKTVPEDLVCIIG
                     PSIGPCCYEVSEDLVEKFNTILTNNDEKFYIIKEGSYYLDLWKINEYMLRCSGVKKEN
                     IINLNLCTSCRADKFHSYRKHNKASERIGTVLQIK"
     gene            complement(18403..18864)
                     /gene="nrdR"
                     /locus_tag="PC700_000552"
     CDS             complement(18403..18864)
                     /gene="nrdR"
                     /locus_tag="PC700_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HDF2579876.1"
                     /translation="MQCPYCNYKESKVIDSRHTDLKSIRRRRECESCKKRFTTYEKIE
                     TTPLMVIKKDNSREYFDREKIKYGLLKACEKRPVSIEEIESIVVHIENEINKCFIEEI
                     ETKKIGEMVMDKLKELDEVAYVRFASVYRQFKDINTFVNELKSILIEKGDK"
     gene            complement(18886..19149)
                     /locus_tag="PC700_000553"
     CDS             complement(18886..19149)
                     /locus_tag="PC700_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HDF2579877.1"
                     /translation="MIRVSDIMEKEIINVKNGKRMGFIIDIDMDIHEGKVVSFTIFGD
                     GSRSFFSRGGDEEVIFWNDILKIGCDTIIVNIGSELSLDEFEI"
     gene            complement(19253..20026)
                     /gene="sigG"
                     /locus_tag="PC700_000554"
     CDS             complement(19253..20026)
                     /gene="sigG"
                     /locus_tag="PC700_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HDF2579878.1"
                     /translation="MQVNKVEICGVNTSELPVLKNKQMKELLLQIKNGDEEARQQFVR
                     GNLRLVLSVIKKFNNRGENIDDLFQIGCIGLIKAIDNFDLSQNVRFSTYAVPMIIGEI
                     RRYLRDNNPIRVSRSLKDIAYKALQVRERLIRTNSKEPTVSEIAKELELEVESVVMAL
                     DAIQDPISLFDPVYQDNGDAIFVMDQVQDKKDTDENWLQEISLKEAIKKLNSREKLVL
                     DLRFYKGRTQIEVADEIGISQAQVSRIEKNALKNMRKYV"
     gene            complement(20118..20840)
                     /gene="sigE"
                     /locus_tag="PC700_000555"
     CDS             complement(20118..20840)
                     /gene="sigE"
                     /locus_tag="PC700_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HDF2579879.1"
                     /translation="MLRLKERIISFITMLGIKLKIIKPKGIYYMGGANILPPPLKPEE
                     EMELLQKLETDESVKSILIERNLRLVVYISRKFENTGIDVEDLISIGTIGLIKAVNTF
                     KLNKNIKLATYASRCIENEILMYLRKNNKKKTEVSFDEPLNIDLDGNELLLSDVLGTE
                     NDEIYKIIEEEIDRDLLVMALDRLSDREKQIMELRFGLIDKGIEKTQKEVAGMLGISQ
                     SYISRLEKKIISRLQKEMKKFV"
     gene            complement(20865..21737)
                     /locus_tag="PC700_000556"
     CDS             complement(20865..21737)
                     /locus_tag="PC700_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HDF2579880.1"
                     /translation="MFKGFKGGYMVYIEYYVIENLVINYIIISCTSILTKRYNPKKNK
                     LLGASLGSAYSVAYLYPSMNILFTVPFKIIIMSIITLISFRSKSKKDYVHVALVFYLV
                     NIFISGSTYFIIYFTGISHLKISFLIACTYVSCELLKYIYKDLKMLKYIKELTKTINI
                     NLLEKSFCCKALVDSGNLLKDPISQSDVIIVKSSVLEGFIPEKLLNINYEDIDMKKAQ
                     EIIGSLDDKTSGRVRIIPYKHAGSNKTSIIIGLKADYIEVDEQKIGNIILGISNFNDR
                     EYGAIMNPNILPGF"
     gene            complement(22028..23302)
                     /locus_tag="PC700_000557"
     CDS             complement(22028..23302)
                     /locus_tag="PC700_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDF2579881.1"
                     /translation="MIKLKKVLSLVMVGILSVSILAGCSKKEDSKGKEGKEKLVVWSA
                     PLTDHDADAWKPIFEKFEKENNCEIEFQIVPWDNYAEKYATAISAGEGPDIGYMYAEM
                     FPQFIEMGAVEDLTPYLEKSGTSDNYLYLDDAKMMGGIYGLPIEAANPGVLYYNKDIL
                     EKLGEKPPKTWDDFKRICEKATKDTDGDGKIDQWGLAQGWGSKVFGSLNWNWYPYLWQ
                     AGGDIYNDDLKSVKFNNAEGLKAANFINDMKKYIPEDALSKDSNEMIESVFGPGKAAF
                     TIMLSSAATSTFDKSFPDLNWGYVTSLEDKTVGSFASVDDLTLMSACKNKELGFKLLE
                     YMLSKESMEAFHKEIPRAPVAKGEKYQGDPRFEEMVTRDKHMYRPLTVGPHGVEIYEY
                     LWKQLQMMIAGDITPEQALNESAKYANGLLAQ"
     gene            complement(23586..24743)
                     /gene="ftsZ"
                     /locus_tag="PC700_000558"
     CDS             complement(23586..24743)
                     /gene="ftsZ"
                     /locus_tag="PC700_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDF2579882.1"
                     /translation="MLNFDVELEECAQIKVIGVGGGGNNAVNRMVEAQLKGVEFISVN
                     TDKQALYTSKAEYKVQIGEKLTRGLGAGANPEVGKRAAEESKDEIVKLLQGADMVFVT
                     AGMGGGTGTGAAPVVAGLAKEMGILTVGVVTKPFAFEGKIRMKNAEGGIAELKSKVDT
                     LITIPNDRLLQIVQKNTSMLDAFAVADDVLKQGIQSISDLIAVEGLINLDFADVTTIM
                     KDKGLAHMGIGSASGETRAIDAARQAIQSPLLETSIQGAKGVLLNVTGGPNLGLFEVN
                     EASTLVMESCDPEANVIFGASIKEDLGDEIMITVIATGFEGLQNGALDLDTKPKSSIR
                     SSLNTTVKQAVKEIEEEVIAEEKIEPPKKASIIEEDDDESMEIPTFLRRRR"
     gene            complement(24933..25286)
                     /locus_tag="PC700_000559"
     CDS             complement(24933..25286)
                     /locus_tag="PC700_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HDF2579883.1"
                     /translation="MLWVLGGLAFGVVVGYYIPFTYSMDYSIYMSVAILAIMDSIFGA
                     IKSNFEDNYDNVIFITGFIGNAILAILLTYIGEKLGVSLYYVAVLVFGMRLFNNLSII
                     RRYIISNIMNKKRKN"
     gene            complement(25385..26059)
                     /locus_tag="PC700_000560"
     CDS             complement(25385..26059)
                     /locus_tag="PC700_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HDF2579884.1"
                     /translation="MKKKIVILGAFILGIFISTYIKSLDPNRVYITLGQTESIESEVE
                     NTKKEIKNLKETLKNNKKTLNEYKEAKKNGNESIQTLMQKELEEVKELSGYSTVTGPG
                     ITITMRDSERELKDGQNPNDLIIHDIDILRVLNDLKKAGARAISINGERVLATSKIKC
                     SGATITVNDTTYGQPFIIKAIGDIDLLSAAINSPESYAKSLKDIYGINIKVQENNGNK
                     VNLFAK"
     gene            complement(26064..26762)
                     /locus_tag="PC700_000561"
     CDS             complement(26064..26762)
                     /locus_tag="PC700_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HDF2579885.1"
                     /translation="MKKRFINKSIIIGSVVLGVLVSLQLKIINIENRGMTTFKRGEQL
                     TEELKSLKTEKENLQSEVNSVKKDIETYRGKNNKGEQDILKSEIEKYETLAGYTDVEG
                     TGIKIEINSTKDLGNSDKNDSILYNYDLLLSMINKLNSAQASAISINDQRIVFNTYMY
                     LKEDKLCMNDTVIKEPIIIKAIGDSDTLASALNIKYGIVWEIETYYNAKVKVTKEDNI
                     EINGHSDINKAEGK"
     gene            complement(26785..27525)
                     /locus_tag="PC700_000562"
     CDS             complement(26785..27525)
                     /locus_tag="PC700_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HDF2579886.1"
                     /translation="MKKRRKLNTNNVMIVLVFILMLSFCICIIVKSDLFDVKKIDVIG
                     NKRVTKSNIMKELNINLNENIFAYNFKDMKNKLIKNPYIENVEIKRKLPNKIIISLKE
                     KEIFAVLKDEDNYCYIDKKGNLLEELRGSNESKKDLIVDVDYSVDDNKSIKFKNYKTK
                     ENVFKTLNYLKEEGIYRKINYVNLKKESNIEMLTRSNIKILLSNDDNLDYNISRVSKI
                     LIDLQNKNTNGGTINLNYGKLAVYSPEG"
     gene            complement(27795..29021)
                     /gene="murG"
                     /locus_tag="PC700_000563"
     CDS             complement(27795..29021)
                     /gene="murG"
                     /locus_tag="PC700_000563"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDF2579887.1"
                     /translation="MKVLLSGGGTGGHVYPAIAIANKIRDEHPDAEIIFVGTEKGIES
                     EIVPKYGFELKTVTVQGFKRKIDFDNVKRVFKLFKGLEQSRKIVKKFKPDIVIGTGGY
                     VSGPVLFNASMGKIPAIIHEQNSFPGVTNKILSKTVTKVLTSFEDSHKRFPEAAEDKL
                     VFTGNPVRKEILLSRKNIARKNLSISDEKRMVLCYGGSGGSRKINDAMRLVIKNMVNE
                     DIAFIFATGKSYYDEFMGSISDINLKPYQKVVPYLEDMANALAASDLVIGSAGAISLA
                     EITALGKPSIIIPKAYTAENHQEYNAKSIEKQGAGIAILEKNLTPESLNTAVFKLLGD
                     RELLVDMANASKTIGKPEAIDLIYDEIMKVYNSTQKSTSKKTKKEKVIKEVKEIKKET
                     TPSIEGQAKVIGIKKR"
     gene            complement(29039..30169)
                     /gene="spoVE"
                     /locus_tag="PC700_000564"
     CDS             complement(29039..30169)
                     /gene="spoVE"
                     /locus_tag="PC700_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein E"
                     /protein_id="HDF2579888.1"
                     /translation="MPKENLKKQIDIRMKTEFDGVVFYTTMLLVFVGIVMVFSASFIQ
                     SSFKHNDAYYFLKKNVIYAVLGFIVMIITSRIDYSFWKKNATAIGAIAVVLLLLVLTP
                     LGIEANGAKRWLGIGALTFQPAEIAKFATIILTAKLIEKNYDKIKSLTKGVVPLLVVP
                     GIFFALIILQPNLSTAGTVILVTFVMIFVAGMDMKIVFAMIGSGAALFAALVIAEPYR
                     LSRVTSFLDPFQDPLGKGYQVIQGLYALGSGGLFGLGLGKSKQKYFYIPEPQNDFIFA
                     IIGEELGLIGCIIVIMLFVVLVYRCVRIALKTSNVFACMVVIGIGAQIGIQAALNIAV
                     ATSSMPATGVALPFISYGGTSLTIFMGAVGIVLNISKHVKIN"
     gene            complement(30221..31576)
                     /locus_tag="PC700_000565"
     CDS             complement(30221..31576)
                     /locus_tag="PC700_000565"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDF2579889.1"
                     /translation="MDLKGKKVLLVGLAKTGISTIKHLDKLGASIIVNDIKDENKLRN
                     ILDELKSINDIKYILGHHPEDVDDIDMVVVSPGVPLDLPFILKLKNSGKYIIGEVELA
                     FKLSNNPIFIGITGTNGKTTTTSLVGEIFSRAKRDTYVVGNIGNPVIDTIETSSEESV
                     LVTELSSFQLESIDEFRPKVSAILNITEDHLNRHHTMEKYIEAKANIFMNQTVEDFCV
                     LNYDDEVVKSLADKCNAKVIYFSRTKKVNGGVYLENNDIIIDIDDKIKFLNKDDVSLP
                     GGHNLENCMAAIAIAYVCKIDLEVIKDVLMTFKGVEHRQEFVRNLDNVIYVNDSKGTN
                     PDSTIKAIQSYDRPIILIAGGMDKGSNFDELLETAKSYVKSLVLLGETASNIENCAKN
                     KGFNDIHIVKDMEEAVKTSYEISKSGDIVLLSPACASWDMYESFEVRGKDFKDNVNNL
                     K"
     gene            complement(31595..32563)
                     /locus_tag="PC700_000566"
     CDS             complement(31595..32563)
                     /locus_tag="PC700_000566"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDF2579890.1"
                     /translation="MMLGITELTYTALIAFLIVIIIGPIFIPMLRKFKFGQTVRDDGP
                     QTHLAKNGTPTMGGIIMIVAILITGLTRVKVSHDMAVGLICIAGFGFIGFLDDFIKIK
                     LKRSLGLKAYQKIILQVALSFYVAFYQYTSSSSASQLMIPFTDFVINVGILYIPIMMF
                     IIVAIVNAVNLTDGLDGLASGVTLIVSVFFMLFASSIAGNTEVAVLAAATVGACLGFL
                     GFNSYPARVFMGDTGSMALGGAVVAFSVLTNSVLIIPIIGGIYFAEALSVLIQVGYFK
                     ATRKRFFKMAPIHHHFEQCGWPETRVVFIFWIITVVLAWISIIAVF"
     gene            complement(32604..33977)
                     /locus_tag="PC700_000567"
     CDS             complement(32604..33977)
                     /locus_tag="PC700_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDF2579891.1"
                     /translation="MECLTIKELVLATNGKLIYGDYNDCVSDIVIDSREASAQNAFVA
                     IVGENLDGHTFMKPAYDSGCKTFIKNESNGIKLESSDINLIEVKDTSLALGDISKYYK
                     EKFDIPFIGVTGSVGKTTTRDMIYSAISAKLNILKNEKNLNNHFGVPLTLFNLNKEHE
                     CAVIEMGMSGFNEIKYLVDIVNPKIAVISNIGLSHVEKLGSQEGILKAKMEITSNFDE
                     TNTLIVNGDDKFLSTLKEKEHVYKLKTFGFNKNNDIYCESYTMEEDSLTFICVINGKK
                     EEIFIPTIGEHNIYNAMSAILVGLCLNISLDYIKKGLKNFKGTKMRLDIIKNEKLTII
                     NDSYNASPDSMDAALKILGRYKGRRVAILGDILEMGEISEYGHRLVGKSSMNNTDIII
                     TIGENSVFIGEEAKQLGFNSANIYHFENREDVFNKLNELVKTGDTILVKGSRGMRLEK
                     IVEYLNK"
     gene            complement(34144..36123)
                     /locus_tag="PC700_000568"
     CDS             complement(34144..36123)
                     /locus_tag="PC700_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HDF2579892.1"
                     /translation="MRKVKRISKKRLVLVLILACALFFCLVIRTGYLQLMKGNWLSTK
                     ALEQQTRDIPIEPKRGTIYDRNMKELAVSVTKYTVWCKPVEVEDKKEAAEKVAEILDE
                     DYKDIYALISKKNMALVKVKRWIDDDKASQIRDAKLSGIWVAEDNQRYYPYGNFAPYV
                     LGHTSSDATGISGVEMQYDKKLKGKPGKLIVSTDASGREIPQGMEKYYEPVQGNGLVL
                     SIDEVIQHYTEKAVQKAYELNNAKKVTAIAMNPKTGDILALASKPDYDPNDSRTPIYP
                     YYQEELEKYNDKDKIKGYYQMWRNPAVSDTYEPGSTFKLITSSSALEEGVIKDGEKFT
                     CTGSVTVGGRKIKCWRHYRPHGTQEFKQAVQNSCNPVFVELGSRLGVGKMYDYIESFG
                     LMDKTGIDLPGEAKGILYNEKNVGPVELATISFGQSISVTPIQLITAISSIANGGDLM
                     QPRVVKSYTDNKGNITETVKPKKVRSVISKETSKKMLEIAESVVTEGGGKIAYIPGYR
                     LGGKTGTAQKVIDGKYAPGKYICSFVGIAPCDDPQIVVLAIVDEPTGVSAFGSTTAGP
                     IVKEIMNDSLKYLGVKPVYKEEEKAEYEKKQVKVPDVRNLKIGDAVKALEDAKLKPDL
                     DADIELPEDTKVKDIFPKPGVKVNEDSSITLYFEN"
     gene            complement(36135..36482)
                     /locus_tag="PC700_000569"
     CDS             complement(36135..36482)
                     /locus_tag="PC700_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2579893.1"
                     /translation="MKKNYKIHNLNEYKNKKKNNYKKRNKKKIGAKCILLIAIFSVII
                     LNLCGYSKISEMKYDIYYLQKDLRKKEIVLEELKAKETEKTSIKEIELKAKELLNMDY
                     PKDGQIKYIDVDN"
BASE COUNT        11292 a         6852 c         3864 g        14758 t
ORIGIN      
        1 tttataatta ttatttatta ttccatcata atcacttata tttgacttat ttttatttgt
       61 atcttgattt ttgttagaaa tactttccct ataaggaaaa gaatgttcat tggtaccaaa
      121 tactgatttt gagcttaaat cataattatc atcattttct gtaattggat ttttattgta
      181 aaatcctttt tctttatcag aattattttc tttattaccg tctttaggta tatcatgagg
      241 atttttattt tcattgctat cagtacttga ttctacatca tctttaggag tatttggttg
      301 tttctcatca gaattattat tttcattatt attgttttga ttgttattac tatcattatt
      361 ttcatttcca ttattcccat cttgattgtt atcatcctca tctctatccg ggtcgtctct
      421 acctcctcct ccacggtcac catcaatatc atcattttcc cgttcatcat ctggcgtctc
      481 ttggttgtct ccgcctccca ttataaatcc tggtttaaaa actatatttg aatacccttc
      541 ttttaatcct cctaccactt ttactctaaa acaacgcaac ttaaaatctt caaaaaaaag
      601 tatagcttga tctctaatat cctcctgttc ttcagatatc caattgattc catcgcttga
      661 atattcaaca aaaaccttac ttgcactata cggcatatca taaaataaaa cagctacata
      721 tccatttttt gtattttgaa attccagttc caaattgtct attgtttttt tctctataac
      781 agatacattc aaaataggaa ctacatcatc atttattata attttttctt tatttaacat
      841 ttctgtttta aactttcctg ttatatcaaa attttctccc ttttccaact tttcataata
      901 atcagtaaaa tcccattcta catctatatc aagttgatta ccactagatg tttctaatac
      961 catatatttg ggaaatttta tattttctat tttatcatct ttacttactt ttatttcatc
     1021 actaaataca ccaattcccc tatgacaaca tttcacaata ttatagccag aatctttagc
     1081 tatagagggt ataagctcat tataatcatc aattatataa actttcttac tttctgatat
     1141 aattgattgt gccaacttat tattatctct tagtaaccca ctttcattgt tgctactata
     1201 tgcttttatt gaagtttctt ctatctcaac atcaccttta gaataaacac ctatagcatg
     1261 ttctccattc acttcaatat ccttatttag tattctaata tcattttcag tataaattcc
     1321 tattgcatct tttccatttg cctttatttt ttcaacagat tcgtaggatg atttaatatg
     1381 taagtctcct ccttcttcaa cgtataaggc tgtcccatca gtagcaatta tgttgtttat
     1441 accaatactt atacttccac cattttctat atgtattact ccttcttttc ctccttcacc
     1501 tattatattc aattcattat catcaataat aaatctccca tgatttttta tcaatatttt
     1561 atatttgttg gtatctattg taaaattact gtcataagga atataaaatc taaactcttc
     1621 atctattacc atattacaag aaagagtata aactatttct tctttattat ttttattttc
     1681 ttgaacccac ctttcaaaat cttcaagaca attttcctct tgtccaaaaa ctttatgtgg
     1741 aaaacttata aaacacaaaa ctattatact cataaaaata aatatctcat actttttcat
     1801 cttacactcc tagaaatttt ttagctttga aacaatcctg tcgaaacata tacttcaccc
     1861 actataattt ttccacaatt atcaattatt gtcaatttat ataagtatct ttattaattg
     1921 ttataaataa aaagctactt actaaagtaa acagacgttt cactttaata agtagctata
     1981 tttttataac aaaatagtta taatattaga acttccttca tttacaattt tttgtaaagt
     2041 cttttgcatt ttaactctta tctcttctgg catagtatat aatttacttt gtaattgctc
     2101 ttttactaag tcatgtagtg acttaccaaa catatttgat tcccataaat ctgctggttg
     2161 ctcttcaaat acttccataa gatattttat catctcttca ccttgatttt gatttccaac
     2221 tattggagag acctctgtag atatatctgc ttttataata tgaagtgatg gtgcattggc
     2281 ttttaacttg ataccatatt gttttccttg tttcattatt tctggttctt ccagactaag
     2341 ttcttctaaa gaaggagcta ctactccata tccttttatc ttggcatcta ttaaagcact
     2401 ttctatttta tcatactcat ttttaacttt agatagtcta gttatcagac taagcagttg
     2461 ataatctcct tcaattttaa atccactttt ttcttcaagg acattataga ataaatcttg
     2521 ctttgtctgt aaatcaatat ttataacacc ttctcctagc tctacattat caacacctgt
     2581 atcttccaaa aactctaact cagaaaatcc ttgtataata ccttctatat ctcttatttt
     2641 acctatatct ataatacttt gttttaatgt tgttattatg ctacttttta tccaatgatt
     2701 tctttcaaga ccttcaaccc attttggtaa gttgattctt atttctgtaa gtggaaaatc
     2761 atataataca gcttccataa cctcctctat atcttcttct tccatttcaa ccacattcat
     2821 tggaagaact ggaacttcat acttttcttc caattcactt ctaagcatac tcgtttcctc
     2881 actttttgga cttaacgtat ttaaaaccac tgcaaatggt tttttaagat ttttcaattc
     2941 ttgtataact ctctcttctg gttctacata gctttttctg tcaattccag taactgaacc
     3001 atctgtcaag acaactatcc ctattgtaga atggtctttt ataacttttt tagttcctat
     3061 ctctgctgcc ttttcaaatg tcatagcttc ttgtgaccaa ggagtggata ctaatctttg
     3121 ttttccacct tcctcatgcc ctaaagctcc ttcaactatg tatccaacac aatctaccat
     3181 tcttactttt aaagatactg tgtcttttat ttttatttct acaccatctg ctggtacaaa
     3241 ttttggttct actgtcatta tcgttttacc tgaaccactt tggggtatct catctcttgt
     3301 tctatccttt ttaaattcat tgtctatatt aggtataacc aacttttcca taaattttct
     3361 tataaaagtt gattttcctg ttcttacagg tccaactact ccaatatata tatccccctg
     3421 agtccttttg gatatatctt cgtatatgtt attattcata ttattcctcc tatacatcta
     3481 atcttattaa attatattta gagttaaggg ttaatattct taaaatagaa aaataaagac
     3541 cagcaatatt aatttaattt acatatagaa atttgtaaat ttcaatgtaa attaaattaa
     3601 tattgctggt ccaaatagta gtgtattata ttaactgact ttagtagtat tttcatttga
     3661 cttattattc atctcaataa gttctttacg tctttctttt gtcatgttca tccatataaa
     3721 tgtaaacacc aatccaatta tggctactat agtaagaact atgaagtagt acctatatcc
     3781 agttacaccc ggaaatgcat ccaatatctt ccctgctgct aaaggacaaa gtacttctgg
     3841 aagatatcct aatgtagcaa ctattcctat agcagttcct gatattgata atgaataatt
     3901 accttcttca agtaacgaat agtgtaaacc ttgaattgca tacatagata catatattgc
     3961 aacacatcca atcaatagta tataaatcat agatgatttt gttggtgtaa atattacacc
     4021 taaaagacct gcaatcataa gtataaatcc ataagatata atctttgatg aacctacttt
     4081 atctccaagt aatccagaag cagctgatgc tactggtcta caatactgag ctaatattga
     4141 gattgcagat gcaaacacca cagtagctcc aaatgcttca gttgaataag gcgtaaaata
     4201 ataaaatgac atattagctg aatatgaaca aaacattatt attgttatta tccatgtatg
     4261 aggcattttt attactgcaa aaaaggcttc tttatcaaat tttgctgata cctctttttc
     4321 attatccttt aatttgaaaa atactaatac tccaactatt acatttattg ccgagtataa
     4381 tactataata gctgtaagac cagatttatc acttactttt gatgaaatat acccaaatag
     4441 tatcaatact agagatagat gaattgcatt ggtaattcca cgaccacttt ccataaatcc
     4501 aaaagctttt ccttgttcat tttcagatgc taacattcta acagctttta caagagctgg
     4561 ccaaaaagta agtattgttg ttattcccca taaggcatgg attaaaaaca caacaatata
     4621 tggaggataa agtgttaata caagaccaga tattcctgta ataattaaag aacctgaaag
     4681 caattttctg gctgaaatac ggtctgctaa aaaaccacca aaaaggtatg ataccattgc
     4741 aagtagacca taaatgcttc ctaagcttcc catttgtgta tttgtaagat taaatgcttg
     4801 tgcaaaagta tcataataat aattctttaa atatggtaaa gcatatataa atgctcctgc
     4861 tacaaagagt attatcaaga ctttgaaatt tttccaaatg cttggtttat ttacttcaac
     4921 tgacatactt cctccttatg taaatacctg taaattttca tgataattaa ttattgctta
     4981 ttttattgtt tattcaatat ttcggaatat ttattaatat attaggttaa tctatcaata
     5041 atttatggtg agttatgagc taaaagtaaa ctcttttagc tcaccataat aattatattt
     5101 ttatttatac taacgtccag agttattaga tatctttttt tcatagttct tccatagcat
     5161 taaacctgtt attataacaa aaagacctcc tcctatcatt tcaatctcat agattatatt
     5221 atcttttaaa gtctccataa ctggtgccgt tgagaaaagt atgccaacta cagttactac
     5281 aagtaccatt ctggcaatat tgatacaaac tgaatcactt tttgcaattg aatatggtct
     5341 ttcttcattt ggtcttgtct ttctaagtct aatataagat gtaaaaagca agacatatgg
     5401 aaataatgct gttaatgctg tcatagtaac aagaacattg tatatattat ctactgatgg
     5461 taataagttt gttcctacaa gaagtagact cactattata gcctgaacta ttactgcatt
     5521 tgatggtata ttgtatttat ttgtaacagt caattgctta ggaaatacac cttctcttac
     5581 atttccaaaa agcatcttta ctggacctgc tatataaagt attaatgctc ctagtactga
     5641 taaagttatt ccaaatgcta ctattcttat aaaccaactt cctataccta aatcttgtgc
     5701 aactttagct aatgcatcta gtatccctgt agacgctgca attttatctg gaggaagtat
     5761 cattgttatt gctactgaac ctaagatata gattcctcct attaaaacag ccacagttaa
     5821 tatagcttta ggaaagtttt tcttagcatt atccatttcg gttatgaaat tagcagtcgt
     5881 ttcagctcct gataatgcaa atattatagc agatatagaa actaatgaat tggcatctat
     5941 ttttggtatt atattttgga ttgtatatat cgatgctgat ggagcttttt tgagaataac
     6001 tacagacata aatgccatca ctataagtaa tatagcagga attgtagaac ctaatgctcc
     6061 tgtattcgta aaaaattttc caaatgccat cccacgagta cttactaagg acaacagcca
     6121 aaatattatg agtgaaagtg ctaaaataaa catcttattg tttgataaat ctggatttcc
     6181 aagcatatat gcaatattta tagctaaaaa tgtcaagaaa gaagaatacc aaaatatctt
     6241 tgatgtccaa tttagccatg acaccataaa tccccacttc tctccatagg cttgtttaac
     6301 ccattctcca agtccaccat cttttggata tgttgcagcc aactctgcac atataagtga
     6361 tgcaggaaca aagaatataa aagcaaacaa taaccaaact ggtatagaac caagacccaa
     6421 tccaaaacta gaggcagtag atttagctat ccatctaatc ccaaatagtg ctgatatatt
     6481 cataaatact aaatcccaaa atccaatttt ctttttcact gtaattcctc caatttcaaa
     6541 tttattcaaa ctcatacaca atgtattgat ttaaatatca agtataagtt cttcaacact
     6601 tcttggataa ttagttataa ttgtagcacc attcttacct actattgctg tatctgagtg
     6661 tctaaatcct ccaactccag gtatatatat ccctggctcc atagaaaata ccattccttc
     6721 ttctaatatt aattcattgt caaatcttaa atatggctct tcatgttctg ataaacctaa
     6781 accatgacca attctatgat ttgaatataa ctctagacca tactttgcaa caacagctct
     6841 tgctgcttca tccaccattc ttgctggtat accagctttt attgtatcta atccagcttg
     6901 ttgagcctct actgcaatct taaacacttc tttttgtctt tctgttggtt tacctataat
     6961 aaaagttctt tcattttctg ctctatagtt ttcataccaa acctgtctac tatgtattac
     7021 tatatctcct ctttgaagta tcctagtatt ggaatctaaa tgaggttgtg ctgttctatc
     7081 aatccctgaa caagtccagt ttgcaaatcc tatatatgta tcaggataat tttcagaaac
     7141 atattttagt aaagcattat cccccgcaac gtcaaactcc aattcactca tcccaactct
     7201 aacattttct aaagaccctt ttattcctat atcagatagg taacctgcta tcttcaaaaa
     7261 ttctatttct tttgcatctt ttacatatct catttcaaat attttacttc caacatcttt
     7321 gatagcaaat gacttatcag ttatatactc tgaaaaactt gcagatacta tatctttttc
     7381 tattccaaca gttgttcctt tagggtattt atttaaaatt atatctaagt aatgtttatg
     7441 tgatattcca tgttctttca tttctggtat ttcataatac acatatacat tatcaacttt
     7501 tgcaacttct ttagcatgta attcttctaa tccaggtact attaattcta ttttatcttg
     7561 agttactact gttacaattg gtctagaata agttattgct ttaaatccac taatataaaa
     7621 ttgattgtct ggttcaaata ttaaagcaat ttcaatgctt ttttcattca tgtactttct
     7681 caatctttct agtctcatat tatttgacat aatgcacctc tttcttaata ttcagataat
     7741 ttattcaata caacaaggat aaatatatta ttttaaaatt cttaatattt ttagtacttg
     7801 tcgtatttgc atgtaaatta attttatttg tgattatttt atcataatca tgattatttt
     7861 gtcaatcacg attttatttt taaaattttt ttatattcta gtgattttaa attatctaaa
     7921 atataacgag tatacattgt attttggaat attattcttg atttatggta agtttaatga
     7981 taaaatcacg attatataaa ctatattgat tatttgtagg tgaataaaat gacaaataaa
     8041 gaattacaaa agaaaaggat attgatgtat ttcatagaag ctgctcaaaa tattatggaa
     8101 aacgaaggta tagaaaatat tacacttcgt aaggttgcag atatggctgg atataatagt
     8161 tcaactctat ataaatactt taaaaattta gaccatttaa tatcatttgc atctataaaa
     8221 tattttaaag attataactt gaatatttca agatgtatag aaaatgtcaa tgatgaatat
     8281 aagaaatata taattatgtg gaaattattt tgtaagcatt cttttgacaa tgctcaagct
     8341 ttttatcagg tattttttaa cttgtctagt gatgaactta gctatataac aaaacaatat
     8401 tatgatatgt ttccagaaga tttaggtatt catgatagtg acatatcttt aatgattaca
     8461 ggagaaagta taaaggaaag aaataagata ctattaaaca atcttgtaga tactggatat
     8521 ataccagcta aagatttaga cataatcaat gatataatta tttcttttta tcaaaatcta
     8581 ttattcttaa aaaagaacac aggaaaaaat tctggtgatg atgaattaac tatgaaaatg
     8641 atttcttcta ttgaatacat aataaaacat gctaaataaa attaaaatat ggctgtctca
     8701 aaatagagat aagccttttt tattgttgaa ataataaaaa tatatttaat ttcatataaa
     8761 aaaaagtgcc tcgtaaacta aaaagttcat tttgagacac ccctaaaaca aatttattat
     8821 ttattataca atagtttata tatcatcacc taaaactact tcttccattt catgcttttt
     8881 acttctcatc attagttcta ttccaacttc tttaggattt gaacctttat ttattacaga
     8941 atatatagca ttagttatag gcatatctat atctaatttt tcagcaacat catgtgctac
     9001 ttcagtagca gttatgcctt ctacaaccat ctttacttct tttaaagtat cttctaaact
     9061 cataccttta cctatgagta taccagctct tctatttcta ctatgcatac ttgtacaagt
     9121 aactattaaa tctccaatac ctgaaagacc tgcaaaggta gacatattag ctcccatagc
     9181 aattccaagt ctgctcattt cactaatacc tcttgtcata agggcagctt ttgcattatc
     9241 accataccct aatccgtcac atatcccagc tccaaatgct ataatatttt ttaatgctcc
     9301 tccaagttct actccaacta tgtctggatt cgtataaact ctaagtttag ggctcataaa
     9361 taaatcttgt atcatctgtg ctatttttaa atctttagaa gcaactacta cggtagtagg
     9421 aatatccctt gctacttctt cagcatgaga tggcccagat agtattacat atggattttg
     9481 tggtaattct tcttcacaga cttgagataa tctaagccca gtcccttttt caagaccttt
     9541 agctacatta acaataactt gtccttcttt tataaatggt tttatttttt ggcaagtact
     9601 tcttacagcc tgtgatggaa ctgctaaaac tattacctta caatctttta tagttttttc
     9661 tatatcagta cttatagtta tattgtttgg aaataatact cctggtaaat agtctgtatt
     9721 ttctttagtt ctgtttattt ttttagcttg ttcctctttt cgagtccaca tgctcacatc
     9781 attacctttt tttgcaagcc ctaaagctaa tgcacttccc cagcttccag tacctaatac
     9841 acatactttc tccatgttta acaccacctg agtataatat atttactatt ctttcttttg
     9901 accaattttt ctctctgtcc catttaataa tcgctttata tttgctctgt gattatatat
     9961 tactgatgca gttaagaaca gagctactat taaaccagct ttatttttta ccataatcat
    10021 aaatattggt gaacatccta ttcctacaac agaacccaat gaaacatact ttgttatagc
    10081 taccaccaat ataaatactg ctagacacat tagtgttatt actggattta cagctagcat
    10141 tgcacctagt gaagttgcaa cacctttacc tcctctaaat cctaaaactg ctggccaatt
    10201 atgacctgct acaacacata taactgccag atatccagct aaaagagtat ctattccaac
    10261 caatcttgct gcaaattctg atattaaaac agccattaca cctttaagaa catcacctaa
    10321 aaatgtcatt gctcctgctc tttttccaag tgtcctaagt acatttgtcg aacctgcatt
    10381 accagaacct tgagtcctta tatcaacccc agcaattcgc tttgcaacta tgtatgaggt
    10441 agatatattt cctaaaagat atgcaactac agcaataatt atataactaa atatctccac
    10501 tgaatttccc ccctttagtt atattttaag atatttattt tttatttttt tgtctatact
    10561 caaactttat ggaagttcct tcaaatccaa aattttctct tatcttattc tcaaggtatc
    10621 tttgatatga gaagtgagtt aaatccttat catttataaa caatgtaatt ttaggaggtc
    10681 taatgtcagt ttgagtccca tagtaaattt ttagtcttct acctttgtca gatggtggtt
    10741 gatttaacat tacagcttct ccaattactt cattcagtgc tgaagttgat attctcttag
    10801 aatgttcatt tgacacatat tcaactgtat ctagtatctt gttcattctt tgattagttt
    10861 ttgcagaaac aaatacgatt ggagcataca tcataaatgg aaatttttct tttatatctt
    10921 ttgtgtaatt actcatagtc ttattatctt tctctattaa atcccattta ttgattacaa
    10981 atatgcatcc ttttccttca tcatgtgcta tacctgcaac ctttgtatct tgttctgtta
    11041 caccttctaa ggcatctatt actatcaata ctacatctgc tctatctaca gcactcatag
    11101 ctcttattac actgtatttt tctattgtct catatatttt actttttctt ctaagccctg
    11161 ctgtatctat tagaagaaat ttctgtccat ttttttcaaa ataagtatca attgcgtctc
    11221 tagtagttcc tgcaattgga cttacaatta ctctttcttc tccaagtatc ttattgagta
    11281 tagaactttt tcctgcattt ggtttacctg ttatagctac tcttattata tcttcttcat
    11341 actctgtatc taatcctgct ggaaaatttt caactatctc atctaataaa tctccaaatc
    11401 ccatactatt ggctcctgaa actgcaaaag gcgttccaaa tcccaactca taaaaatcat
    11461 atatattatc aaattgactt tgactatcaa tcttattaac cactaagatt actggttttt
    11521 ttgtctttct aagcaattgt gcaatttctt tatcagcagc tgttattcca gctttaccat
    11581 caactataaa aagtattaca tgtgacatgt ccattgcaag catggcttga tttctcattt
    11641 gagataatat tatatcttcg ctatcaggtt caattcctcc tgtatccact aatgtaaaat
    11701 atttatctaa ccactctact tcagcaaata ttctatctct tgtaactcca ggtgtatttt
    11761 caactattga tatccttttt cctgcaaatt tattaaatat tgtagactta ccaacgtttg
    11821 gtctcccaac tacagctaca actggcctac ttatactcat tttatttctc cttccatctt
    11881 ttgttttcta ttttaaaatt ttatccacaa agtcttttcc ttcattcaaa caagggataa
    11941 tttttattct cataagttct tctaattgat ttaattcaat attatctaag aaaatttctt
    12001 catctgcttt tagcatactt cttgttatat aaagagcttc tcctaattct ttatcttcaa
    12061 gttgttcttt taaatcctta gctgttaaaa gacctgatac agttattgtc tctccaaaaa
    12121 acttgttttt gatttcatat acatttactt gaagattttt aaacttgtcc atcatagcat
    12181 ctgccatact ctgtataaat tcatatgcag aatgaccagt agctattgat actttcttct
    12241 ctctttttag aatattctca ggcaatatat ttagatactc ttcaatctct gtttttaact
    12301 tacgtatcat acctacacca tcttcaaact gtaaaaaacc ttcatattct tcatatccag
    12361 gcaattcact attagatagt atataaaatt catctgataa aaatgcaaat cgagtaccta
    12421 atttttcaag atatttctgt tgtatttgat gtatttgctc tatagtttta cttgcgctct
    12481 tatcattaaa tatatttaat tctactagat tttctctatg ctttgtaatc ccaactggaa
    12541 ctatcgcaac actgtttaca tatggataaa gttgagctag ttcttttatt gttctatcca
    12601 attcctttcc atcatttata cctggacata aaacaatttg acagttcatt tctatatgag
    12661 catctgccaa tctcttcatt ataccatata attttcctgc aaatttattg cttatcattt
    12721 tttgtcttaa ctctggatta gttgtatgca cagatatatt tataggactt attctatatt
    12781 ttataatatt atttatatct tcttcactca tatttgtaag tgtgacaaaa tttccttgta
    12841 aaaaagataa tctcgaatca tcatctttaa aataaagagt ttctctcata ccttctggca
    12901 attggtctat aaaacaaaac atacacttat ttctacagct tttagcttgg tcaattatcg
    12961 gatttgtaaa ttcaacaccc aaatcttcat catagtcttt ttctatctcg tatatgtaga
    13021 cttcgccatc ttttttttgt atttctacat ctaaatattc atcacttagt aagaatctat
    13081 actctattat atcatgtatt ggctgttcat tcacagatat aagtaaatct cctacctcta
    13141 tacctatttc ttctgcaata ctatctttat aaaccttact tataatatta tttacttttt
    13201 ttacatttac ttccaatatt ttcaccactc ttttgtattc atcaatgctt attataatat
    13261 attttaatat gcaatttatt tatttattat attttatgat agcattttta agcatttcat
    13321 gtcataataa cacattttaa ttctaatagt caatataagt aaatagctat aaaatagatt
    13381 agaacaaata atattcaaat ttaagtttct taaaaatttg aatattattt gttcttaaaa
    13441 aaattttata aatttaatgt tttactatga ttaacgtccc atttgtcata tcatgaacca
    13501 ttccacctac acactttgac atcaacaatg ccttattttg taagtctttc tcatcatttg
    13561 cataaaatat tggacaacct ccaccatttt tcatattctt atcaagtgta actatagcta
    13621 gggtatattc atttaaacct atatccattc ccatcttatg cacttccttt ctttcctatt
    13681 ttataatttt ttagtgataa gttttttcct ttagagcttg aaagtattgg gcatgacttt
    13741 acagcttcca ttactttttc catattctta tctactggta aataagcaat cattatactt
    13801 tcatcctctt tatttcttct aggtaatggt acgaatgaag gttctccata atctccatat
    13861 aaacctaacc ttgaatatat attgtatact attgcttgtc tttgaccgga gtcaaataat
    13921 attcctgcat tagcataatc cttattttta ggaattattt gtattcctac acctctatta
    13981 atatatactt ctctatcact ttttagacct atatttgtta taccttttaa atctcccacc
    14041 tttagtatag attcgtccac aaaactaatt tcagcaggca ttacatcagc tatgtctcca
    14101 atactttttc tagtaagaag tttttttaat ataaaggcta acactaatcc acaaattgaa
    14161 cttattacca aactcatccc ttcacttatt ttcgtttctg caagtattac ataatacaat
    14221 cctattgtta aaaaagatgt aactatacac atataatttc ttacttcata ggttctagct
    14281 atctcttcta tgaatgcagc acctctcttt acaagttgaa cttcctctaa attttgcaat
    14341 gtatttcttc tatttgctct cacttgtcta aattgttctg ctgctagaga taaaaatgtt
    14401 atggatgtat aagaacgatt tataagagct ggaaccaata atgctcctaa tgaagaagcg
    14461 acgaatgcta atataagttg agatagtaaa acatttggtt gcgttggata ctgtttttgg
    14521 tctaaattca acattataat tctagatgtt ataccaataa taacagcaac aaaaaatact
    14581 ctatccatta aatcactcct aaaatattta atttgaaatt ttacatgtaa atctacaaag
    14641 tctttctaga aaaatcactc tgctttcctt ttgatgtttc tatgataggt gtacttttca
    14701 ctacatctat catgacatct atatctttga gtattggcat atatggtata accacagtac
    14761 ttttttctaa attggtctta gatatagcaa caatatcaac ctcatctaca tctctatcta
    14821 tccccatatg aataaataga ttatgtatta tagcttgtct ttgccctata tcatttacta
    14881 ttccaaatgc acctaaatct cttggtataa cttctatggc cagacattca tttctatact
    14941 tttctcttgt actttctaga cctatattag ttattattac atcatttacc attaaaatag
    15001 gtccttcaaa acttattttt gcaggaacta tatctgctat atctgctata ctgtttcttc
    15061 ttaaaaatct tctaaaaatc aagccaacaa tagctcctga cacaatagct aaaattgtgc
    15121 aatgaaaaaa gctaaggtca tactttcttg caaaaacgat ataaactatc gaagatacta
    15181 gtgcagaaaa aagacttata taacttctag attcatacgt agatgctatt tcttcaacat
    15241 aagcatctcc ttttggtact aattcttcat tatcaatatt ctttagtgtt attctttctt
    15301 gttccgcaag accttgaaac tgttgtattg ctactgcaaa aaatgtcaag gctgcaaact
    15361 ccttatctat aagtgcaggt aatgctatag cacctaacga agctgaaagc ccagatataa
    15421 ttatctgttc taaataatct tgtggtcttg atgggtattg tttgtctgta actcttaaaa
    15481 ctaatcctct acataatatt cctacaacta atgcaactat aaaagagtgt ctaaataaat
    15541 ttgcattcaa taaactttct tccattttct cagctccttc tcttatatag tttgtgcaaa
    15601 aaacaaaaaa aattcccttc tatttaagga aatttttttg taatattgta actataaatt
    15661 cgctaccaat gccaagttca ctttttactt ttatacatcc atttagtgat ttgactatat
    15721 gctttgttat agccaatcct aaacccgttc ctcctacatc cctacttctt gctttatcta
    15781 ccctataaaa tcgttcaaat attcgttcta catcttcttt tggtatccct acaccattgt
    15841 ctataacttt aattacgatt ttttcttcat cttttaacac tacaacttgt acaatcccat
    15901 tatcttgggt atactttata gcattatcaa ctaggtttaa gaacacttgc tttatatagt
    15961 ctttattgga tagtatactt attgatttat catcacattt atataaaact tctatatttt
    16021 tttcactagc tgaatgttga gttatttcat atacctcttt aaatacatca tatactacaa
    16081 cattttccat aagaagagta tctttatttt ctacaaatga taacagcaat atatcatcaa
    16141 tcaacctttt taatctatct gattcgcttt ctattatttc taagaatcta tttctagtat
    16201 tcacatctat attttcattt gactttagtg tttcaacaaa cccacttata gatgtaagtg
    16261 gtgttttaag ttcatgactt acatttgcaa caaaatcact tctcatattt tcaagtttaa
    16321 cttttttagt aatatcttct atatttacta tcgaacctat tattacgttg ttagtattct
    16381 gaagataaac tgggtctacc tttatcttat atacgatatc atctttagta gtaatattag
    16441 cagtttcatt atctttagaa ccaataaatt gaagtatttg ttttaatatt tcttcttctt
    16501 ttattacaac attaatatta tgaccttcaa ctaattgatt tggttcactc tttatcattt
    16561 cttttgcttc atcatttatc agaagtatat ttccatttac atctatagcc aaaattccat
    16621 gagatatact ctttaatata gatcttaact gtaaatgttt atactgtact tcttctaagg
    16681 ttttatccat attctttatc atatgattaa aatttctagc taattcacca agttctcctt
    16741 tggctgatat gttaagccta gcatgatatt cattattact tacttttttt gagacgtata
    16801 taaactcttt taaatatttc cttaatgtta ttgtatatct tacagctaat ataacaatgg
    16861 ctatggacaa cataacaata aaaaaatata tactatttaa atcttccaca agataacact
    16921 cctaaaatat aaccttttta atctatttta taccctacac ctcttatagt ctctatatat
    16981 ttttccgatt tatcttcatc ttctattttt tttcttaaat atcttatatg aacatctact
    17041 gttctagttt ctccatagta ttcatacccc catattttat ccaaaagata atttcttgaa
    17101 agtacttttc ctttattttg aattaataat tttaacaatt caaattcctt aagagttaac
    17161 tctatctttt cagagccttt ggtaacttca tgttttgata aatctagttt taaattgcct
    17221 gtagtaagta tattgttttc ttcacctaag ctagtcaaat tatatctcct taaaacagaa
    17281 ctaatcctag ctaacaactc ttttatacta aatggcttag ttatatagtc atcagcacct
    17341 atttcaagcc cttcaacctt gtcattttcc atatttttag cagtaagcat tataacagga
    17401 atatttttta agtccttatc acttcttatt tttctcaaaa cttctattcc acttatattt
    17461 ggtaacatcc aatctaaaag tattaaatct ggtttaattt ccttagcctt tataaatccg
    17521 tcaaatccat catatgaata actaacttcg taatttgaca cctccaaatt gaattttaat
    17581 agctcaacta tatgcatttc atcgtctata acaagcactt tagtattcat taaaaagctc
    17641 ctctctattt tatctgtagt accgttccta ttcgttctga tgccttatta tgttttctat
    17701 aagagtggaa tttatctgct ctacaactag tgcataaatt taaatttatt atattttctt
    17761 ttttaactcc actacatcta agcatatact cattaatttt ccataaatct aaatagtaac
    17821 ttccctcttt tattatatag aacttttcat cattatttgt aagaattgtg ttaaattttt
    17881 ctactaaatc ttcagacact tcatagcaac atggtccaat tgatggccca attatacata
    17941 ctaaatcttc aggaactgtt ttatagcatt cagacattaa ttttattgtt ttatgaccta
    18001 tattactaaa agtccctctc caacctgcat gagccagtcc tatggcttta tttttagcat
    18061 ctataattga tataggtaca cagtctgctg taaacagtaa aagtggtact ttttccaagt
    18121 ttgttataag tgcatctcca tctcttcttt tccctatagt atctttattg actatattaa
    18181 ctatatctga atgtatttga gaattactag ttatattttc aaatgtcaaa tcacattctt
    18241 tacatacttt taacatatct tctctatttt ttgcatctat attcttacat gttataacta
    18301 gatttacaga atttaatttt tcatcaagtt cttcacaatt accacttaaa ttaacatact
    18361 ctttaatatc ttgtacattt tttatattta tataatcttt catcatttgt ctcctttttc
    18421 tatcagtata cttttaagtt catttacaaa agtatttata tctttaaatt gtctatacac
    18481 agaagcaaat cttacatagg caacctcatc taactctttc aatttatcca ttaccatctc
    18541 accgattttt ttggtttcaa tttcttctat aaaacattta tttatttcat tttcaatatg
    18601 tacaacaata ctttcaatct cttcaataga aacaggtctt ttttcacatg cttttagcaa
    18661 tccatatttt atcttttctc tatcaaaata ttctctacta ttatcctttt taatgaccat
    18721 taaaggagtt gtttctattt tctcatacgt agtaaacctc tttttacatg attcacactc
    18781 acgtcttctt ctgatagatt ttaaatctgt atgtctagaa tctattacct ttgattcttt
    18841 ataattacaa taaggacatt gcatctatat tcctcctttg tttaactata tctcaaactc
    18901 atctaagctt aattctgaac ctatatttac tattattgta tcacaaccta ttttaagtat
    18961 atcattccaa aatataactt cttcatcccc tcctcttgaa aaaaaacttc tacttccatc
    19021 tccaaaaatg gtaaatgaga ctaccttacc ttcgtgaata tccatgtcta tgtctattat
    19081 aaaccccatc ctttttccat tttttacatt tataatttct ttttccatta tatcagaaac
    19141 tctaatcata ttgtactcac cctttcttta aaaataaata tgtatcgaag taaaaaaaaa
    19201 tgacctatat aggtcattta aattaaaata atgtttacag tccatggatt acttatacat
    19261 attttctcat attttttaaa gcattttttt caattcttga tacttgtgct tgagatatac
    19321 cgatttcatc agcaacttct atctgagttc ttcctttgta aaaccttaaa tctaatacca
    19381 acttttctct actatttaat ttcttaatag cttctttaag tgatatttct tgcaaccaat
    19441 tttcatcagt atctttttta tcttgaacct gatccataac aaatattgca tctccattgt
    19501 cttgatatac tgggtcaaac agagatattg ggtcttgtat ggcatcaagt gccattacaa
    19561 ctgattctac ttctaattct aattcctttg ctatttctga tactgtaggt tctttagaat
    19621 ttgttctaat taatctttct ctaacttgta gtgccttata tgctatatct tttaatgacc
    19681 tacttactct aattgggtta ttgtctctta gatatcttct aatttctcct ataatcatag
    19741 gaacagcata agttgaaaac cttacatttt ggcttaaatc aaagttatct atagctttta
    19801 taagaccaat acaacctatt tgaaataaat cgtctatatt ttctcctctg ttgttaaact
    19861 tttttattac actcaagact aatcttaaat ttcctcttac aaactgttgt ctggcttctt
    19921 catcaccatt ttttatttgt aataaaagtt ccttcatttg cttattttta agaactggaa
    19981 gttctgatgt attaacacca catatttcaa ccttattaac ttgcatatat tttcagtcct
    20041 ctctcaaaag tattcatcta ttgaaattat ttacatttcc aattttttta tactttttaa
    20101 gaagaattaa tttaacatta tacaaatttt ttcatttctt tttgcaacct tgagattatt
    20161 ttcttttcaa gtctagatat gtaggactgt gatattccaa gcattccagc aacttctttt
    20221 tgtgtttttt caataccttt atcaataaga ccaaatctta attccataat ttgcttttct
    20281 ctgtcactta atctgtcaag tgccataacc aacaaatctc tatctatttc ttcttcaatt
    20341 attttgtata tttcatcatt ttctgtaccc aatacatcag ataataaaag ttcatttcca
    20401 tctaaatcta tatttaaagg ttcatcaaat gatacttctg ttttcttttt attatttttt
    20461 cttaagtaca ttaatatttc attttctata catctcgaag cataagttgc caattttata
    20521 tttttattca gtttaaaagt attaactgct tttatcaaac ctatagttcc tattgaaatc
    20581 aaatcttcta catctatacc tgtattttca aattttctcg atatgtagac tactaatcta
    20641 agatttcttt ctattaaaat tgactttaca ctttcatctg tttctagctt ttgtaaaagc
    20701 tccatttctt cttctggttt caatggagga ggaagtatat ttgcccctcc catatagtat
    20761 attccctttg gctttattat ttttaattta atacctaaca tagtaataaa gcttattatt
    20821 ctttctttca atcgtaacat tccttgtccc ccttaaattt tcacctaaaa tccaggcaat
    20881 atatttggat tcattattgc tccatattct ctatcattaa aatttgaaat accaagtatt
    20941 atatttccta tcttttgctc atctacttct atatagtcag cttttaagcc tattattatg
    21001 cttgtcttat tacttccagc atgtttataa ggtattattc taactctacc tgaagtttta
    21061 tcatctaaac ttcctataat ttcttgtgct ttcttcatat ctatatcttc ataattaata
    21121 tttaatagtt tttctggaat aaatccttct aacacactag acttaactat tattacatca
    21181 ctttgactta ttgggtcttt taacaaatta ccactatcta ctaaagcttt acagcaaaaa
    21241 cttttttcta gtaagtttat atttatagtc tttgtaagct ccttaatgta cttaagcatt
    21301 ttcaaatctt tataaatgta ttttaataat tcacaactca cataagtaca tgcaattaag
    21361 aaagatattt ttaagtgact tattcctgta aaataaatta taaaataagt gcttccagaa
    21421 ataaaaatgt ttactagata aaaaactaac gctacatgaa cataatcttt tttgctcttg
    21481 cttctaaatg aaattaaagt tattatgctc atgattatta ttttaaatgg tacagtaaat
    21541 agtatattca ttgatggata caaataagca acagaatagg cacttcctaa actagccccc
    21601 aataacttat tttttttagg attatatctt ttagtaagaa tagaagtaca acttataata
    21661 atataattta ttactaggtt ttctataaca tagtactcaa tatacaccat gtatcccccc
    21721 ttaaaacctt taaacatatt ataagatagt atgtacaaaa ttttatgtca cttttgatgg
    21781 tcgaatccaa aacaaattct atttaaaaag aacaatgata tatgtcttat actttatgtc
    21841 gtcaaaagat atttttttaa tgccaaacag atagactttt ttgtagaatc caatatatct
    21901 aggtagaagt tattcgcttt acttttggta tatttataaa aaaaagacta cataatttaa
    21961 taatatatca agtaaatctt ttacttatat aaaatcaaat tatatagtct gtaaattcaa
    22021 atataaatta ttgtgctagt aatccatttg catattttgc agattcattt agtgcttgtt
    22081 ctggagttat gtctccagct atcatcattt gaagttgttt ccataaatat tcatatatct
    22141 ctactccatg aggtccaact gtcaatggtc tatacatatg tttatctctt gtcaccattt
    22201 cttcaaatct tgggtctcct tggtattttt cacctttagc aactggagct ctaggtattt
    22261 ctttgtggaa agcttccatt gattctttgc ttaacatata ctcaagaagt ttaaatccaa
    22321 gttctttatt tttacatgct gacattaatg ttaaatcatc tactgaagca aaacttccaa
    22381 ctgtcttatc ttctaatgat gttacatatc cccagtttaa atccgggaat gatttatcaa
    22441 atgtacttgt agcagcagag gatagcatta tagtaaatgc agcttttcct ggtccaaata
    22501 cactttcaat catttcatta ctatcttttg ataaagcatc ttctggaata tactttttca
    22561 tatcatttat aaagtttgca gctttcaagc cttctgcatt attaaattta actgatttta
    22621 aatcatcatt atatatatct ccaccagctt gccataaata cggataccag ttccagttta
    22681 agcttccaaa tactttagaa ccccaacctt gtgcaagacc ccactggtcg atttttccat
    22741 ctccatcagt atcttttgtt gctttttcac agattctttt aaaatcatcc catgtttttg
    22801 gaggtttttc tcctaatttt tctagtatat ctttattgta gtacaatacg cctggattag
    22861 cagcttctat aggtaaacca taaataccac ccatcatttt tgcatcatct aagtatagat
    22921 aattatctga agttcctgat ttttctaaat aaggtgttaa atcttctact gcacccattt
    22981 ctataaattg agggaacatc tctgcataca tgtatcctat atctggacct tcacctgcac
    23041 ttattgctgt tgcatatttt tctgcgtagt tatcccatgg tactatctga aattcaattt
    23101 cacagttatt ttctttttca aatttttcaa atatcggctt ccatgcatct gcatcatggt
    23161 ctgttaatgg tgcagaccat acaactaatt tttctttacc ctctttgcct ttagaatctt
    23221 ctttttttga acatcctgca agaattgata ctgatagaat ccctaccata actaaagaaa
    23281 gaaccttttt tagtttaatc atgcttaatc ccctttcaga ctttaatttg tctttttcct
    23341 caaatgttta catttaattc agatttaata ttaacaaata agactactct tgtctaatca
    23401 taaaaaacta tactaactcc tataactata ttttttttct atacctatta gatttaattc
    23461 atactaaaaa cttacttttt aaattttatc aatatttaaa aatattttta agtctagcca
    23521 caaactaact atctaaattg aacaaaataa aaagaagtct attgacttct ttataagctt
    23581 acatattatc ttcttcttct taggaatgta ggtatctcca tgctctcatc atcatcttct
    23641 tcaattatac ttgccttttt aggaggctct attttttcct cagcaattac ttcttcttca
    23701 atttctttta cagcttgttt tactgttgta tttaatgaag aacgtatgct tgatttaggt
    23761 ttagtatcta aatctaaggc accattttgt aatccttcaa atcctgttgc gattactgta
    23821 atcattattt catcacctaa atcttctttt atagaagctc caaatataac atttgcttct
    23881 gggtcacagg attccataac taaagttgat gcctcattaa cttcaaataa tcctaagtta
    23941 ggaccacctg taacattaag aagtactcct ttagctcctt gtattgatgt ttctagaagt
    24001 ggagattgaa tagcttgtct tgcagcatca attgctctag tttcaccact agcactacct
    24061 atacccatat gagcaagtcc cttgtctttc attatagtag taacatctgc aaagtccaag
    24121 tttattaacc cttcaactgc tataaggtct gaaattgatt gtataccttg ttttaataca
    24181 tcatctgcaa ctgcaaaagc atctaacatt gatgtatttt tttgtactat ctgtaaaagt
    24241 ctatcatttg gtatcgttat aagagtatct acttttgatt taagttctgc tattccacct
    24301 tcagcgttct tcattcttat cttaccttca aatgcaaaag gtttggtaac tacacctact
    24361 gtaagtatcc ccatttcttt tgccaagcct gcaaccactg gagcagctcc tgtaccagta
    24421 ccaccgccca ttccggctgt tacaaagacc atatcagctc cttgaagtaa ctttactatt
    24481 tcatctttac tctcctcagc agctcttttc cctacttctg gatttgctcc tgctccaagt
    24541 cctctagtta atttttctcc tatttgaact ttatattcag cttttgatgt atataaagct
    24601 tgtttatctg tatttactga tataaattca acgcctttta gttgagcttc taccattcta
    24661 ttgacagcat tgtttccacc gccacctaca cctattactt taatttgtgc acattcttct
    24721 aattctacgt caaagtttag catcataaac ccccttctct catgattcaa agttatcgta
    24781 tgtattaatt atatcatttt cttcttttat tttgttaacc tttaagtaac atattccaca
    24841 aaaacgtaaa ttttcctttt taaatttctt acttatattg tcaaatatta ctctttaagt
    24901 ataaaataat ttacgtatat attaaatgct ctttaatttt tacgtttttt gttcataata
    24961 ttactaataa tatatcgtct aattatagat aagttattaa acaatctcat accaaaaact
    25021 agtacagcaa catagtaaag agaaactcct aacttttctc ctatataagt caataatata
    25081 gccaatatag cattacctat gaaaccagta ataaaaatta cattatcata gttatcttca
    25141 aaattggatt ttatcgctcc aaaaatagaa tccattattg caagaatagc tactgacata
    25201 tatatggaat aatccataga atatgtaaat ggaatatagt aacctactac tacaccaaat
    25261 gctaatcctc ctaaaaccca caacataaat cttcacctct ttattttact ttgaaacaca
    25321 actacattac aatccttttt attaattttt tttacttttt ctttattttt ttgtattttt
    25381 cgttttattt tgcaaataaa ttcactttat tcccattatt ttcctgaact tttatattaa
    25441 ttccatatat atccttaaga gatttagcat atgattctgg agagtttata gctgcactca
    25501 ataaatctat atctcctata gcttttatta taaatggttg tccatatgta gtatcattta
    25561 ctgtaatagt agctcctgaa cattttatct tacttgttgc tagtactctt tccccattta
    25621 tagaaattgc tcttgctcca gcctttttta aatcatttaa aactctcaaa atatctatat
    25681 catgtataat caaatcattt ggattctgtc cgtcttttaa ctctctctca ctatccctca
    25741 tagtaatagt tatgcctggc cctgtaacag ttgaataacc acttaattct ttaacttctt
    25801 caagctcttt ttgcataagt gtttggatag attcatttcc attctttttt gcttctttat
    25861 attcattcaa agtttttttg ttgtttttaa gagtttcttt taaatttttt atttcttttt
    25921 ttgtattttc tacctcactt tcaatacttt cagtttgacc taatgttatg tatactctat
    25981 ttgggtctag tgattttata taagttgata tgaaaatgcc taatatgaat gctcctaata
    26041 taactatttt ctttttcata ctattatttc ccctctgctt tgtttatatc actatgacca
    26101 tttatttcta tattgtcttc tttggtcact tttactttgg cattataata tgtttctatt
    26161 tcccagacaa taccatactt tatattaagt gccgaagcca atgtatcaga atcaccaatt
    26221 gcttttatta taattggttc ttttatgaca gtgtcattca tacaaagctt gtcttctttt
    26281 aaatacatat atgtattaaa aactattctc tggtcgttta tagatatggc acttgcttgt
    26341 gctgaattta atttatttat cattgacaat aataaatcat aattatatag tatactatca
    26401 tttttatcag agtttcctaa gtcttttgta gaatttatct caatttttat gcctgttcct
    26461 tcaacatctg tatatccagc taatgtttca tatttttcta tttctgactt aagtatgtct
    26521 tgttctcctt tattgttttt gcctctataa gtctctatat ctttcttcac actattaact
    26581 tctgattgta gattctcttt ttctgttttt aaagatttaa gttcttctgt aagttgctct
    26641 cctcttttaa aagttgtcat ccctctattt tctatattaa taattttaag ttgtaaacta
    26701 acaagaacac ccaatacaac acttcctatg attatacttt tatttataaa tctttttttc
    26761 aatatatcac ttccttacaa ttgtctatcc ttctggacta tacactgcca actttccata
    26821 gttaagattt atagtccctc cattagtatt tttattttgc aggtcaatca gaattttact
    26881 taccctagat atattataat ctaagttatc atcattagaa agtaagattt ttatattact
    26941 tcttgtaagc atttctatat tgctttcttt ttttaagttt acataattta tttttctata
    27001 aattccttct tcttttaaat aatttaaagt tttaaataca ttctccttag ttttataatt
    27061 tttaaatttt atagatttat tatcatcaac gctataatct acatctacta tcaagtcttt
    27121 tttactttca ttagaacctc ttagttcttc caataaattt ccttttttat ctatataaca
    27181 ataattatct tcatctttta atactgcaaa tatttctttt tcttttaaac ttataattat
    27241 cttattggga agttttcttt ttatttccac attttctata tatggatttt ttataagttt
    27301 atttttcata tctttaaaat tgtatgcaaa aatattttca tttagattta tatttagttc
    27361 tttcattata tttgatttgg tcaccctctt atttccaatt acatctattt tctttacatc
    27421 aaataaatca ctttttacaa ttatacatat acaaaaagat aacataagta taaaaactaa
    27481 aactatcatg acattattcg tatttaactt tcttcttttt ttcaaatata atcaacccct
    27541 ttggttaata aatacacctt tagagcttgt ttttcctaca aaaaaattat aatatttaaa
    27601 gtcaaatttt tgcattttat tacataacat ccaaataagg tattaaattt tataaataaa
    27661 tcaatatttc atcaaaataa acatagtata ctacatacta tttatatatt taaatctgta
    27721 tacatacaaa taaaccccta atttataata atttagaaaa tctaatatat tataaattag
    27781 gggtaaatat ttacttatct tttctttatt cctattacct tagcttgacc ttctatactt
    27841 ggagtcgttt ctttttttat ttcttttact tccttaataa ctttttcctt ttttgttttt
    27901 ttagaagttg acttttgtgt tgaattgtat actttcatta tctcatcata tataaggtct
    27961 atggcttcag gcttacctat agttttactt gcatttgcca tatcaactaa aagttctcta
    28021 tctcctaaaa gtttaaatac tgctgtattt aggctctctg gagttagatt tttttctaaa
    28081 atagcaattc ctgccccttg tttttctata ctcttggcat tatactcttg atgattttca
    28141 gcagtataag ctttaggtat tattatagat ggctttccaa gagccgttat ctcagctaat
    28201 gaaattgctc ctgcgctacc tataaccaaa tcacttgctg caagtgcatt tgccatatct
    28261 tctaagtaag gcacaacttt ttggtatggt ttaagattta tatcgctaat acttcccata
    28321 aattcatcat aatagctttt gcctgtagca aatataaaag ctatatcttc atttaccata
    28381 ttctttatta caagtctcat tgcatcattt atttttcttg aacctccact accaccatag
    28441 caaagtacca ttcttttttc gtcacttatt gatagattct ttcttgcaat attttttctt
    28501 gataataaaa tttcttttct aactggattt cctgtaaaaa ctaatttatc ttctgctgcc
    28561 tctggaaatc ttttatgcga atcttcaaaa cttgtaagta cctttgtaac tgttttagat
    28621 aaaatcttat tagttacacc aggaaaagaa ttttgctcat gtattatggc tggtattttc
    28681 cccatagatg cattaaataa aacaggtcca ctaacatatc caccagttcc tataactata
    28741 tctggtttaa atttcttaac aatctttctt gattgctcca aacctttaaa aagcttaaaa
    28801 actcttttta cattatcgaa atctattttt cgcttaaaac cttgaactgt tacagtcttt
    28861 aattcaaatc catacttagg aactatttct gactctattc ccttttccgt cccaacaaaa
    28921 attatctcag cgtctggatg ctcatctctt attttgtttg caatagctat agctggatac
    28981 acgtgccctc cagttccacc gcctgataat aaaactttca tcaaatcatc atctcctgtt
    29041 aattaatttt gacatgcttt gagatgttta acactattcc tacagctccc ataaagatgg
    29101 tgagtgaagt tcctccataa cttataaatg gtagtgctac acctgtagct ggcattgatg
    29161 atgtcgcaac tgctatattt aaagctgctt gtattccaat ttgtgcacct atacctataa
    29221 caaccataca agcaaataca ttagaagtct tcaaagctat tcttacacat ctgtagacta
    29281 aaactacaaa tagcataata actattatac aaccaatcaa tcctaactct tctcctatta
    29341 tagcaaatat aaagtcattt tgtggctctg gtatataaaa atacttttgt ttactctttc
    29401 ctaagcctag accaaacaat ccacctgaac ctagtgcata tagaccttgt attacctgat
    29461 accccttacc aagagggtct tgaaaaggat ctaaaaatga tgtaacacga ctaagtctat
    29521 agggttcagc aattactaat gcagcaaata aagcagcccc actccctatc atagcaaata
    29581 caattttcat atccattcca gcaacaaata tcattacaaa agtaactaga attacagtac
    29641 ctgctgttga caaatttggc tgaagtatta tcaacgcaaa aaatatccct ggaactacga
    29701 gaagtggtac aacacctttt gtcaaagatt ttattttatc ataatttttt tcaattaatt
    29761 ttgcagttaa tattattgta gcaaactttg cgatttctgc aggttgaaat gttaaagctc
    29821 ctattcccaa ccatcttttt gctccgtttg cttctatccc taaaggggtc aaaactaaca
    29881 ataataatac tactgctata gctccaattg ctgtagcatt tttcttccaa aaactataat
    29941 ctattcttga tgtaataatc ataactataa atccaagtac agcatatatt acattctttt
    30001 ttagaaaata gtacgcatca ttgtgtttaa atgaagattg tataaaactt gcactaaaca
    30061 ccataactat tcctacaaat actaaaagca tagtagtgta aaacactact ccatcaaatt
    30121 cagtcttcat ccttatgtca atttgttttt tcaaattttc tttaggcatc caactaatct
    30181 acctccattc ttccatttaa gtaagtttag aggtaagaga ttattttaaa ttgtttacat
    30241 tatctttgaa gtcttttcct cttacttcaa aactttcata catatcccaa cttgcacaag
    30301 ctggtgaaag aagcactata tctccacttt ttgaaatttc ataagaagtc ttaactgctt
    30361 cttccatatc ttttactata tgtatgtcat taaatccttt atttttagca caattttcta
    30421 tatttgatgc agtttctcct aataagacca atgatttaac atatgacttt gcagtttcaa
    30481 gaagttcatc aaaattactt cccttatcca tcccccctgc tataagtatt ataggtctat
    30541 cataagactg tatagctttt atagtggaat ctggattagt tccctttgaa tcatttacat
    30601 atattacatt gtctaaattt cttacaaact cttgtctgtg ttccactcct ttaaatgtca
    30661 taagtacatc ctttataact tctaaatcaa ttttgcagac atatgctatt gctatagctg
    30721 ccatacaatt ttctaagtta tgacctccag gtaaacttac atcatcttta tttagaaatt
    30781 tgattttatc atctatatct attattatgt cattattttc taaatatact cctccattta
    30841 cttttttagt tcttgaaaag taaattactt tggcattgca tttatcagct aaagatttta
    30901 ctacctcatc atcatagttt aacacacaga aatcctcaac agtttggttc ataaatatat
    30961 ttgcctttgc ttctatgtat ttttccatag tatggtgtct atttaagtgg tcctctgtaa
    31021 tatttaatat tgcgcttact ttaggtctaa attcatctat actctcaagc tgaaaactgc
    31081 ttagttcagt tactaacaca gactcttcac ttgaagtttc tatagtatct attacaggat
    31141 ttcctatatt accaactaca tatgtatctc gttttgctct tgaaaaaatt tctcctacca
    31201 aactagttgt agttgtcttt ccatttgtac ctgtaatccc tataaaaatt ggattattcg
    31261 atagtttaaa agctaattct acctcaccaa ttatatactt gcctgaattt tttaatttca
    31321 atatgaatgg taggtctagt ggtacacctg gagacactac aaccatatct atatcatcta
    31381 catcttctgg gtgatgtcct aatatgtatt ttatgtcatt tatagatttc aattcatcaa
    31441 gtatatttct caatttattt tcatctttta tatcatttac gattatactt gcacccaatt
    31501 tatctaaatg tttaattgta gatataccag tttttgcaag tcctactaat aaaacttttt
    31561 ttccttttaa gtccattttt cattccccct agccctaaaa tactgctatt atacttatcc
    31621 aagctaaaac aactgttata atccaaaaaa taaatacaac tcttgtttct ggccaaccac
    31681 actgttcaaa gtggtggtgt attggagcca tcttaaagaa tctctttctt gttgccttaa
    31741 aatatccaac ttgaatcaaa actgatagag cctccgcaaa ataaattcct ccaattatag
    31801 gtattataag cactgagtta gttagaactg aaaatgctac aactgcacct cctaatgcca
    31861 ttgaacctgt gtcgcccata aacactctag ccggatatga attaaatcct aagaaaccta
    31921 aacatgctcc aacagttgct gctgctaaaa ctgcgacttc tgtatttcca gcaatagaac
    31981 ttgcaaatag cataaagaat acagaaacta ttaatgttac tcctgatgct aatccatcta
    32041 atccatcagt caagtttaca gcatttacta ttgctacgat tataaacatc attattggta
    32101 tatataatat tccaacattt atcacaaaat ctgtaaatgg tatcataagt tgtgaagcac
    32161 ttgatgatga tgtatactga taaaaagcaa cataaaaaga taatgcaact tgaaggatta
    32221 ttttttgata tgcttttagt ccaagtgatc tttttagctt tatttttata aaatcatcta
    32281 aaaagccaat aaaaccaaaa cctgcaatac atataagacc tactgccata tcatgactaa
    32341 cttttactct tgtaagtcca gttattagaa ttgcaacaat cattataatt ccacccatag
    32401 taggtgttcc gtttttagct agatgtgttt ggggtccatc atctctaact gtctgaccaa
    32461 atttaaattt tctaagcatt ggtataaata ttggtcctat tataattact attaaaaacg
    32521 caatcaatgc ggtgtacgta agctctgtta ttcctaacat cataaaactc ctctcccttg
    32581 tcaattaact cattagttta agcttattta tttagatatt cgactatttt ttctaatctc
    32641 atgcctcttg agccttttac taaaatagtg tctcctgttt ttactaattc atttaacttg
    32701 ttaaacacat cttctctatt ctcaaagtga tatatattgg ctgaattgaa tcctaattgt
    32761 tttgcttctt ctccaataaa cactgaattt tcacctatag ttattataat atcagtatta
    32821 ttcatagatg attttccaac taatctatga ccatactctg aaatttcacc catttctagt
    32881 atatctccaa gtatcgctac ccttctacct ttatatcttc caagtatctt taaagcagca
    32941 tccatagaat ctggactagc attatatgaa tcattaatta ttgttaattt ttcatttttt
    33001 attatgtcta gtctcatttt tgtcccttta aaatttttta aacctttttt tatataatct
    33061 aaagaaatat ttagacacaa accaactaat atagcagaca ttgcattata tatattatgt
    33121 tctcctattg ttggtataaa tatttcctct tttttcccat ttattacaca tataaatgtt
    33181 aaactatcct cttccatagt ataactttca caataaatat cattgttttt attaaagcca
    33241 aatgttttta acttatatac atgttctttt tcttttaatg tagataaaaa cttatcatct
    33301 ccatttacaa ttaaagtatt tgtttcatca aagtttgaag taatctccat ctttgctttc
    33361 aaaataccct cttgtgaacc cagtttttct acatgtgata atcctatatt cgatatgact
    33421 gcaattttag gatttactat atccactaaa tattttattt cattgaatcc agacataccc
    33481 atctctatta cagcacactc atgttcttta tttagattaa ataaagtcaa tggaactcca
    33541 aaatgattat ttaagttttt ttcatttttt agtatattta actttgcaga tatggcagag
    33601 taaatcatat ctctagtagt agtttttccc acactacctg taacacctat aaatggaata
    33661 tcaaattttt ctttatagta ttttgaaata tctcctagag ccaaagatgt atcttttacc
    33721 tctattaaat ttatatccga actttctaat tttattccat tactttcatt ttttataaaa
    33781 gttttacatc cactatcata tgcaggcttc ataaatgtat gcccatctaa attttctcca
    33841 actatggcta caaatgcatt ttgggcactt gcctccctgc tatctataac tatatcactt
    33901 acacagtcat tataatctcc atatattaac ttcccattag ttgcaagtac taactctttt
    33961 attgttaagc actccattat taaactcctc ctttaaaacc ttagctatat tctcttagca
    34021 acaaaaggca atgtataccg ttatatatac attgcctacg ataactctta actttataat
    34081 tatatactat atttctgttt ttttgaagat gtttatgggt atatgttaat tatctgttac
    34141 tttttagttt tcaaaatata gggttatact tgagtcttca ttaactttta ctccaggttt
    34201 tgggaatata tcctttacct ttgtatcttc tggaagttct atatctgcat ctaagtctgg
    34261 ttttaacttc gcatcctcca atgcctttac agcatctcct atttttagat ttcttacatc
    34321 tggcacttta acttgcttct tttcatactc agctttttct tcttctttat atactggttt
    34381 aactcccaaa tattttaaag agtcattcat tatttctttc actattggtc ctgctgtagt
    34441 actaccaaat gcacttactc ctgttggttc gtcaacaata gcaagaacta ctatttgcgg
    34501 gtcatcacaa ggtgctattc caacaaatga acatatatac tttcctggag catacttacc
    34561 atctataact ttttgagctg tacctgtttt accaccaagt ctataccctg gtatatatgc
    34621 tatctttcct ccaccttcag ttacaactga ttctgcaatt tctaacattt ttttagatgt
    34681 ttctttagat ataacacttc ttactttttt tggttttact gtttcagtta tatttccttt
    34741 attgtcagta taagatttta ccactcttgg ttgcattaaa tctccaccat tagctattga
    34801 tgatatagct gttataagtt gtattggagt tactgatata gattgaccaa atgatattgt
    34861 agccaattca actggtccta catttttttc gttatacagt attcccttag cttctcctgg
    34921 taaatctata cctgtcttat ccataaggcc aaatgattct atataatcat acatttttcc
    34981 aacacctaat ctgcttccta attcaacaaa tactgggttg caagagtttt gtactgcttg
    35041 tttaaattct tgtgttccat gaggtctgta atgtctccaa catttaatct ttcttcctcc
    35101 aacagttaca ctacctgtac atgtgaattt ttctccatct ttaattactc cctcttcaag
    35161 agcactggaa gatgtaataa gcttaaatgt agaacctggt tcatatgtat cacttactgc
    35221 tggatttctc cacatttgat aatagccttt tattttatct ttatcattat atttttcaag
    35281 ttcttcttga taatatggat atattggcgt tcttgagtca ttagggtcat aatctggttt
    35341 tgatgctaaa gcaagtatat cacctgtttt agggttcata gctattgcag taaccttttt
    35401 tgcattattt aactcataag ctttctgtac tgctttttca gtatagtgtt gaattacttc
    35461 atctatagac aacacaagac cattaccttg tacaggctca taatattttt ccattccttg
    35521 tggtatttct cttcctgagg catcagtact tactataagt tttccaggct ttccttttaa
    35581 ctttttatca tattgcattt ctacaccaga tatacctgtt gcatctgaag aagtatgtcc
    35641 taacacatat ggcgcaaaat tcccatatgg gtaatatctt tgattatctt ctgctaccca
    35701 tattccactt aatttagcat ctcttatttg acttgcctta tcatcatcta tccatctttt
    35761 tacttttaca agagccatat tttttttgct tattaaagcg taaatatctt tataatcttc
    35821 atccaaaatc tcagctacct tttcagctgc ttctttttta tcttctactt ccacaggttt
    35881 acaccaaaca gtatacttag ttacacttac tgctaattct ttcatatttc tatcatatat
    35941 agttcctctt ttgggttcta ttggtatatc tcttgtttgt tgttctagtg cttttgtact
    36001 caaccaattt cccttcataa gttgtaggta cccagtccta ataacaaggc aaaaaaacaa
    36061 tgcacatgct agaataagaa cgagtaccag ccttttctta cttatcctct ttacttttct
    36121 caaatctatt ccccctagtt atccacatct atatacttta tttgtccatc ttttggatag
    36181 tccatattga gcaattcctt agcctttagc tcaatttctt ttattgatgt tttttcagtt
    36241 tcttttgcct ttagttcctc taatactatc tctttttttc ttaaatcttt ttgtaaataa
    36301 tagatatcgt acttcatttc cgagattttt gaataaccac atagattcaa tattattact
    36361 gaaaaaattg caatcagtaa aatgcattta gctcctattt ttttcttatt tctcttttta
    36421 taattattct tttttttatt tttatattca tttagattat gtattttata attttttttc
    36481 aaattctcac cccgatagta tatagtttaa attttcctct atactatatt tgttattaat
    36541 catatttctt cctctaaaat ttcatcgcta aattaataca ctattacaca atctaattgt
    36601 atttttttct atacacaaca aaaacctata aattcactta tacttttata tgaatctata
    36661 ggttagatat taatttctat ttatttttat cacttctagt atttttagat aatttcttcc
    36721 ataaatataa tatgtaacta actatctact ctctgattcc taagtt
//
LOCUS       DAMJWP010000012        35579 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.012, whole genome shotgun sequence.
ACCESSION   DAMJWP010000012 DAMJWP010000000
VERSION     DAMJWP010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35579
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.012"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            197..442
                     /locus_tag="PC700_000570"
     CDS             197..442
                     /locus_tag="PC700_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HDF2579894.1"
                     /translation="MKYKTTGTCATEIEFEVKENKVTNVNFIGGCDGNLKGLKVLVEG
                     MNIEDVIQKLKGIECKTKPTSCPDQLSLALENYMNIK"
     gene            477..1202
                     /locus_tag="PC700_000571"
     CDS             477..1202
                     /locus_tag="PC700_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HDF2579895.1"
                     /translation="MKPIIGILGNLIIMENGMFPGLERSYVNNDYINAVLKGGGSPVI
                     IPVNTDKEVIKKQIEMVDGVLISGGWDINPQLYGEETREETTFIYPEVDEFDLIAISI
                     ALELKKPILGVCRGLQILNVSLGGTLYQDNNLIEGSYIKHTQSSKRHVATHKVDVKEG
                     SILEGILGKQLLTNSYHHQSVNQLGKGLKAIAYSKDGIIEAIEKEEENFVVGVQWHPE
                     MMVDYCDKMEKLFKYFINICSNL"
     gene            1224..2537
                     /locus_tag="PC700_000572"
     CDS             1224..2537
                     /locus_tag="PC700_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2579896.1"
                     /translation="MNEKNKMGLISIILLGINAVVGAGVFLLPGDAMKSFGVASIFVY
                     IFDMLLVLSMAFCFAEVAGKFNKNGAAYVYTKEAFGDFCGFEVGLMKWVIGCISWGAL
                     IVGFPTSLSAVWAPAGEPHIQKIIIVAMIVGLTIINLLGVSLSKIVQNVITVGKLIPL
                     ILFIGIGIFFIKGVNFTSSTMVPPGAGATEFGAAALLMFYSFTGFESIAVAAEDMENP
                     QKNIPIAIISVIVIASIIYILNQVVCVGILGDSLSSTSTPVADAARICFGNMGAGLVT
                     FGTLVSVGGICMCGAFVNPRSCVALADDKMLPRIFARKDKKGTPYVAIIATMLITIPI
                     ALSGSFAELAAISAVARFIQYIPTSLSVLVFRKKRPELVGTFKTPFGAVIPLIAVCVG
                     TWLLFQASMHQLIMGLGALAIGVPLYFIMKSYNRKVCGENLKKIV"
     gene            2836..3282
                     /locus_tag="PC700_000573"
     CDS             2836..3282
                     /locus_tag="PC700_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2579897.1"
                     /translation="MGGETHFLSFGKDEYKISLEEQENAIESAKASDNSVKLIAFIDD
                     EIVGIATIDSNQKAKGKHVGVLGIVVKEKYWGIGLGKRLMLDLIEWCKSNGITKKITL
                     VTNEENYNAIGLYKKVGFEVESILKKECYYNGVYTDLIGMSLLLGI"
     gene            3371..5356
                     /locus_tag="PC700_000574"
     CDS             3371..5356
                     /locus_tag="PC700_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HDF2579898.1"
                     /translation="MKNLCKISDDYLNSKLKYLKLLSKQYPSISKASTEIINLEAILN
                     LPKGTEHFITDVHGEYEPFVHVLKNGSGVIKRKIEELFSNTIRDSEKKMLATLVYYPE
                     QKLDLIIKQEENIDDFYRINIYRLIELCKYASSKYTRSKVRKLLPENFKYIIEELLHE
                     HVKSEHKEEYYKSIVETIVDIGIAKEFIIAISTVIQKLVVDRLHVIGDIYDRGPRPDI
                     IVDKLIEHHCVDIQWGNHDILWMGAASGEKTCIANALRISARYANLDIVEDIYGINLL
                     PLATFAIEMYKDDPCKEFIPKVNDQSVTTTEKSLMAKMHKAISIIQFKLEGEVIRRRP
                     EFEMEHRLLLNMINYDEGTITLKGKTYKLKDTYLPTIDKKDPYKLTMEERNVIDKLVS
                     SFRGSEKLQKHVSFLFSKGSIYLKANSNLLIHGCVPLNEDGSFMSMNIMGKEYKGKAL
                     MDKMESLAREGFFFKDKAEEKLYGMDIMWYLWTGKCSSLFGKDDMTTFERYFIEEKET
                     HKENKNPYFKLRENEMACKRLFEEFDLELDESHIINGHVPVESKNGESPIKANGKILV
                     IDGGFSRAYQKTTGIAGYTLIYNSRTLQLVSHEPFNSAEEAIANESDILSTTVVVEHK
                     AKRKMVRDTDEGVKIQEEIEDLKLLLMAYKKGLIKEM"
     gene            5580..6548
                     /gene="spoIIIAA"
                     /locus_tag="PC700_000575"
     CDS             5580..6548
                     /gene="spoIIIAA"
                     /locus_tag="PC700_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HDF2579899.1"
                     /translation="MNKLSDEIINSLSTTIREKVEKVSNNNLNIEEIRLRSQKPLILN
                     ANSKDYFYNQKTMTLDLNQQNSYVVTREDVEQTFQIICKYSIHSFMDDIKKGFITLRG
                     GHRVGLVGKAIVEDGQVKNIKHISSLNIRVSREIIGCSDKILSHIIKGKNQINNTLII
                     SPPQCGKTTLIRDIVRNLSNGNEDYGFKGLKVALVDERNEIAGAYLGVPQMDVGIRTD
                     IIETCPKDLGITMLLRSMSPNVIVTDEIGSEKEIKALYTALNGGIGLITTVHGDSIDD
                     IQNRKELNRLLDKELFKKVIILSAKRGAGTIEKIYDLEEKRWYFAN"
     gene            6538..7059
                     /locus_tag="PC700_000576"
     CDS             6538..7059
                     /locus_tag="PC700_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HDF2579900.1"
                     /translation="MQIKIIIIALLVGSSYLIGEQIYKTYTRRHKQLNDLIRVLEILR
                     MDLSFGLYTLEEIFNRIGGNKEFCFWKFFYQISEGLHNEQSKTLEIIISENIDVLSKE
                     TYLGNKEIEELKNLILTLGKSDIESQQRMIDLSIENLKKQTYETKEDINKKGVLYKKL
                     VTFIGIGICIILI"
     gene            7076..7273
                     /gene="spoIIIAC"
                     /locus_tag="PC700_000577"
     CDS             7076..7273
                     /gene="spoIIIAC"
                     /locus_tag="PC700_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HDF2579901.1"
                     /translation="MEISLILKVAGVGILISVLNMILEKTDRKDWAGLTTLAGVIIVL
                     GMVITEISDLFNTVRTMFQLY"
     gene            7289..7675
                     /gene="spoIIIAD"
                     /locus_tag="PC700_000578"
     CDS             7289..7675
                     /gene="spoIIIAD"
                     /locus_tag="PC700_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HDF2579902.1"
                     /translation="MEIMQLIGIAIISTTLCLVIKKDRPEIANFIAIITGVIILLSVM
                     FKLNFIVDSIQDLANKANIPTMYISLIIKLIGIAYLMEFAIQLCKDCGEGNIASKLEF
                     GGKIIVMSMSFPILLSIVEMVVNIIP"
     gene            7760..8965
                     /gene="spoIIIAE"
                     /locus_tag="PC700_000579"
     CDS             7760..8965
                     /gene="spoIIIAE"
                     /locus_tag="PC700_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HDF2579903.1"
                     /translation="MKKRFLPMIIGFLFTFFFVNIFAIMIFANEVPSSEDKEYGETKN
                     SIDKYIDGQLDKLDINEIQDYINKEIVINDVNLKSFVKDLISGEKNILDLFNKDGLKI
                     LMFDEFKASLKVVAVILVLALLSSILKSLENSFSSGAVSQIATYIIFITMVSLTLIGF
                     KDVLQICYDAIDHTVGLMQVIMPILITFLLLIGFPITSTTLNPIFIGGVTFINVFFKN
                     FLFVSITVAFGILIINNLSKNIRLKRFFSFVKQINYVSIGAMFTVYLGLVSIQGLYVT
                     SFDKFSVKTAKFAIGNFIPVVGGFVSDSVDILLSSSQLIKNIFGGIGLILLVGICLLP
                     VIKILSVIVVYKLAAIIVEPVGEDGISNFLNEVANLMIIMLASVIAITVMFFVTVAIL
                     TSISVVSQG"
     gene            9025..9642
                     /locus_tag="PC700_000580"
     CDS             9025..9642
                     /locus_tag="PC700_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HDF2579904.1"
                     /translation="MLEGIKAWIVSVLIGAFIVNIVDMILPSSKIKPYVNLVLNFMFV
                     FIVITPVVGFFSKDMSLEDRILKSMGNYNKQYVDSTNALAKETGNNSLSKGYEDGLKE
                     VLKLKLDEYGYDLEDIELNGANINNIKIKEKNNSAKSNNSSNEENKNNSTKREEENIN
                     SNDKENSKQVFKKGTEYGLNLNEEKLKNDLIKVLDVSIEDIQIDK"
     gene            9654..10208
                     /locus_tag="PC700_000581"
     CDS             9654..10208
                     /locus_tag="PC700_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HDF2579905.1"
                     /translation="MLKNLNDKDKRKVYSLITIAGICVVSLVLLSCFPSSKSDKQVGK
                     TETNKNAEKQVTKKQEKDDLESKLTAILSKIDGAGDVDVMVTFESSEEIQPAFNSNNT
                     TETTEEKDAQGGERTVTTSSENKTMITSNSSDPVVIKTTEPKIKGVIVVASGASDPSV
                     KETLYSAVQTSLQVAGHQVEIYSK"
     gene            10235..10924
                     /locus_tag="PC700_000582"
     CDS             10235..10924
                     /locus_tag="PC700_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HDF2579906.1"
                     /translation="MKFNYKGRGFVIITLTAMLVVVGTVNYQLSKKSLLETSKEFKAY
                     EEAQLQKNTDDSDSSKTEDSSNVDKQGGKESADIDIVDSKASKVKEKATETSKEIKAQ
                     LSSEKNMKKASYILDMKMNREKQRNELVQDLNEMINNPSTTEESRKEASNMKLNIVKI
                     QEKELQIENLLSTKGYEEALVYISDNKVNVVVNEAKLEKKDAAKIFDLVAEQANVKYE
                     NIKLTNNNSNK"
     gene            11034..11396
                     /locus_tag="PC700_000583"
     CDS             11034..11396
                     /locus_tag="PC700_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDF2579907.1"
                     /translation="MEDNKFGQVKISNDVIATIAGLAALEVEGIETTATLTDKLLKNN
                     GVKIQIEEEDVNLDVMVTIKYGMSIPDTAFKVQENVKNTVETMTGLKVSQVNIHIQGI
                     SFKKDKVDKEEAKATKKN"
     gene            11481..11987
                     /gene="nusB"
                     /locus_tag="PC700_000584"
     CDS             11481..11987
                     /gene="nusB"
                     /locus_tag="PC700_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDF2579908.1"
                     /translation="MKKDRAQKSTTREYIMKLIYQININKEDFETLEDKVDNFLKDNS
                     EHIINRYKELALQYSKNTNLKLEDTEIEDVIDKKYINTVCKALKENHDKIDELINKHA
                     KNWTVDRMPKVDVSILRLSVCEILYLDTPNKVSINEAVELAKIYCDDKSPKFINGILG
                     SVVDEIGK"
     gene            11971..13041
                     /locus_tag="PC700_000585"
     CDS             11971..13041
                     /locus_tag="PC700_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-sialoglycoprotein endopeptidase"
                     /protein_id="HDF2579909.1"
                     /translation="MKLGNNIIIGIDTSCYTTSIAAISLDKKVIFNEKIMLEVRDNSK
                     GLRQSEAVFQHINNLGILSDRIKSFKDKFNVEGVCSSKKPRPVENSYMPVFNVGHNFG
                     KLLSSIYGCRFYETTHQENHIEASLLNSKLKNNNKFISVHMSGGTTEILLTSKQDSHH
                     NVCDTNLGKIAKISIKKDDKSKLYNNFGYNIDIIGGSKDISFGQLIDRVGIKLGYKFP
                     SGKYLDENALNCNLKIESGLKTSVRDGYMNLSGLENQVNKIINDNGDNTNQKEYISKL
                     VLDSVVRNMFKSLVYLCETYNVNEVVFAGGVSASKYILRELSMKLRKKHIEAYFTEPQ
                     YSTDNAVGCAIIGLNNFLGERV"
     gene            13038..14243
                     /locus_tag="PC700_000586"
     CDS             13038..14243
                     /locus_tag="PC700_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDF2579910.1"
                     /translation="MKLRALDISEANSYIKRILINDPILSNLKVKGEISNFKVHSSGN
                     VYLSLKDETSKLNCVIFKSNFNRNLKLDNGVKIIANGYISVYERDGAYQLYINEIEIE
                     GIGNLHIEFNRLKEKLNKEGLFDPKYKIPIPKMPNSIGVITSPTGAVIRDIINVIKRR
                     YPKVNIKLYPVMVQGDKSAEEICEAIRFFNHMKNVDTLIVGRGGGSIEELWSFNDEMV
                     AREVFNSQIPIISAVGHETDFTICDFVSDMRAPTPSAAAEIATPSLDDINYKLGNIKS
                     RMSKSLTNQIELDQYRLETVFNKINNYLDSYTIKDKVIQLDKIYDKIIFGIENNLKLE
                     DEKLVKIGALLHNLSPLATMDRGYSITQKNGKVINSIKGLKIKDSIDIVLKDGNLECM
                     IDKIENKEG"
     gene            14245..14460
                     /gene="xseB"
                     /locus_tag="PC700_000587"
     CDS             14245..14460
                     /gene="xseB"
                     /locus_tag="PC700_000587"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDF2579911.1"
                     /translation="MNLTYEEAYKRLESILSELESKNASLDESLSLYEEGISLYKHCN
                     KLLDDAKLKISKFNQLGIEEDFKIEEE"
     gene            14463..15350
                     /locus_tag="PC700_000588"
     CDS             14463..15350
                     /locus_tag="PC700_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDF2579912.1"
                     /translation="MEFKQCLKEKASFVEKVLKEYMPKEEGYQKTVIEAMNYSLSAGG
                     KRLRPILTLEACKIVGGNEDEAIPFAIAIEMIHTYSLIHDDLPALDNDDLRRGRPTNH
                     KVYGEAMGILAGDALLNYAFEVMLAGSINKENPEKYLKAINEIAKGAGIYGMIGGQVV
                     DVESENKQIEKEKLDYIHMNKTAAMMVGCMRAGATIGGANSEQMEEITKYAKNIGLSF
                     QIVDDILDIVGDEAKLGKKVGSDIENHKSTYPSLLGLDKSKEIAHNLIDEAKKSIEKL
                     SDDVDFLKGLAEYIIDREY"
     gene            15380..15817
                     /locus_tag="PC700_000589"
     CDS             15380..15817
                     /locus_tag="PC700_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HDF2579913.1"
                     /translation="MDFFSEIFNNGALGISLIACFLAQFIKIFTGKEKRIELSRILIS
                     GGMPSSHSSFVTSLATVVGIEKGFNSTDFAIITVLALIIMYDAAGVRRAVGKQATILN
                     QMVADIQHGKHIEQKKLKELIGHTPLEVWFGALLGIVTALILM"
     gene            15851..17716
                     /locus_tag="PC700_000590"
     CDS             15851..17716
                     /locus_tag="PC700_000590"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDF2579914.1"
                     /translation="MYKYLDKVNSPKDIKNMSIEEMDLLAKDIRKFLVKSVSKTGGHL
                     ASNLGVVELTLALHKVFDSPKDKIVWDVGHQSYVHKIVTGRKDCFVSLRQFNGLSGFP
                     KENESPHDIFDTGHSSTSISIATGIACARDIKKENYSVISVIGDGSITGGMALEALNQ
                     LGYIDTNMIVILNDNEMSIDKNVGGMSKYLSSIIRNSTVEKMTDEVDKILNVTQTGEI
                     LSKTAHRFKDKLMYSFSPQDCSFFDSLGIRYYGPIDGHNTKELIDILRKAKHKKGPVL
                     LHVITKKGKGYRFAEEQPDKYHGVSKFDIKTGVTSAKVKSMSISVGEKLVDMANNNED
                     IVAITAAMPSGTGLNLFESAYPKRYYDVGIAEQHATGFAAGLAKNGMKPYFAVYSSFL
                     QRAYDQVIHDVCITKKPVTFLIDRAGLVGNDGETHHGMFDLSYLNSIPNIVVMAPKDT
                     REMELMMDLSLKLDCPLAIRYPRGSSYYLDKGEYGEIVLGKYEVLDDGQDTVILCIGS
                     MVKHALEAKEILSREGINPTIVNARFLKPIDEGMLKALLKNHKNVVTIEDNIVTGGFG
                     SRINKFIIDNEYNVNILNIAIPEEFVKHGNIDELYDFVGLSPKSIADKIRKLVIE"
     gene            17849..18661
                     /locus_tag="PC700_000591"
     CDS             17849..18661
                     /locus_tag="PC700_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HDF2579915.1"
                     /translation="MKKRIDLLLVEQGYFESRERAKKAIMAGLVFVDNQRCDKAGTEV
                     KEDCSIEVKGNPIPYVSRGGLKLEKAMKNFDLTIDGKVCMDIGASTGGFTDCMLKNGA
                     IKVFSIDVGYGQLAWKLRQDDRVVCMERTNIRNVTIEYTKQFADFASIDVSFISLKLV
                     LPKAKELVRHDGEVVALIKPQFEAGREKVGKKGVVREKSTHIEVIKMISDFSVENGFE
                     ILGLDFSPIKGPEGNIEYLIHLRNGNEGYEFDGETYNNKIVEVVEASHNLDK"
     gene            18681..20360
                     /gene="recN"
                     /locus_tag="PC700_000592"
     CDS             18681..20360
                     /gene="recN"
                     /locus_tag="PC700_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDF2579916.1"
                     /translation="MILELYMKNCALVEELRLNIDKNLNILTGETGSGKSIIIDALGL
                     CLGDKYDRSFLRKGTDKGLVEAVFFSDNRYLKKILEENDISMEDDNLLVITRLIYSDG
                     KSTARVNGRTVKVSLLKEIASTLIDIHGQHQNQALFNKDTHLKFLDLFGENELEEFKI
                     AYKNVYHKYSEVKKALNCLTENKDEMQIQREIDLLRFQINEIEAANLNKNEYEDLLKQ
                     REVYRNSEKIYNNLNSSYSKLHNGEYNVIDLIGLASKELNDISKYDSVLSEYSDTVER
                     IMYELQDISGEIRNYKDNIDFEPYELEQIELRIDEINNLRRKYGDSIDDIFEYYGKIK
                     DRLDEILNRDERVEQLKSKLMNIEEDLKIKASKLTKARIEVATTLEKILLDELKSLNM
                     KNVMFKVNFEKGSFTLDGVDDIEFMISFNLGEDIKPIYKVASGGEMSRFMLAFKTILA
                     DIDDIDTLVFDEIDTGISGIAAQIVGEKLSDIAKKKQIICITHLPQIAANADTHYCIE
                     KDTSNNRTFTNVSKLNESQRKNEIARLIAGNNITEKTIEHASEIIELAKKC"
     gene            20575..21441
                     /locus_tag="PC700_000593"
     CDS             20575..21441
                     /locus_tag="PC700_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2579917.1"
                     /translation="MNKKDTMHLIFIIVIFILLWYIVVAVSEINKNIESCSNKLDYLS
                     QELMSTKSDISNTINEEMSKSYITKSIDLKVKKIEKKECTIDVDVQLSKLGKNGKVFF
                     MYKEDSDKWNETEMTKHGELSYACEIKINTGSEYDYKVVTSGDISESSDVQKLTKSDY
                     MPEQPIFNSGIRDNREYFIEIVENSNLFNHESEKSKNKAYDNIRLEKVDIIVNEGKKD
                     TIYKAQLAEGLDDGKTNNSNRNQVNYEVSFPKRDIDDIIYIKAKLTYNNGVEYTKDIT
                     EDITMGLQDLEK"
     gene            complement(21532..22023)
                     /locus_tag="PC700_000594"
     CDS             complement(21532..22023)
                     /locus_tag="PC700_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2579918.1"
                     /translation="MADKMAQNITLQFVEEKDLENLENVKLLFTEYSNSLNIDLCFQD
                     FNNELKTLPGKYKKPSGSLILAFVDENLAGCVALKKLEGKICELKRLYVRNQFRGLKI
                     GKILLEEIIVEAKKIGYTHMRLDTLPSMKSAQGLYEKFGFYDIELYTYNPIEGARYME
                     LKL"
     gene            complement(22084..22524)
                     /locus_tag="PC700_000595"
     CDS             complement(22084..22524)
                     /locus_tag="PC700_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2579919.1"
                     /translation="MKLNVRIADINNWHDLVSLSVDKSQLDYIESNALSIAESKFITA
                     WVPVGIYDENSLIGFAMYGRLEDDRVWLDRFMIDSKYQGKGCGKASLDFLVNHLKNEY
                     NCDELYLSIFEDNKMAIKLYKDFGFKFNGELDYGGEKVMVLKSN"
     gene            22685..23743
                     /locus_tag="PC700_000596"
     CDS             22685..23743
                     /locus_tag="PC700_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HDF2579920.1"
                     /translation="MNKINKNFKNIWIILGAVSIFTMVIIFNKNCNVNKETQIEAFTI
                     KNHDKKFVYPMGNIIGVKANTDGVLVLGYEEEDVGYIGGIQIGDNIVKIDNKRIKNSQ
                     DVSEILNKIKKSKVEVTFERKNKYKTEVIETKKENGKYKLGLWVRDKISGIGTMTFYD
                     PSMEKFKAIGHAIKDSDTNELLKIKQGYIYKPEQLKIVKASNEKVGKIKGDFNDSNLM
                     GNFSNNSELGISGNITENHNKEFNVANKEKQLIEVGRPQDVKIGDAVILFEDKNKNIT
                     SYDIKIESIVYDKGNYRDMVIKVVDDKLLEYTGGIVQGMSGAPIIQNNKIIGAITHVF
                     RDNPKKGYGIFIDEMIKL"
     gene            23885..24709
                     /gene="spo0A"
                     /locus_tag="PC700_000597"
     CDS             23885..24709
                     /gene="spo0A"
                     /locus_tag="PC700_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HDF2579921.1"
                     /translation="MGGFLVEKIKIVLADDNKDFCQVLKEYLSNEDDIDILGIAKDGI
                     EALDLVKKTQPDLLILDVIMPHLDGLGVIEKLNTMDIPKMPKIIVLSAVGQDKITQSA
                     INLGADYYIVKPFDFVVFINRIRELVSNRVTQVEPKPRPVQETQMTRSDFVKNVGNIE
                     TEITNIIHEIGVPAHIKGYLYLREAIKMVIDNVELLGAVTKELYPSIAKKFNTTPSRV
                     ERAIRHAIEVAWSRGKVDTINQLFGYTVHNTKGKPTNSEFIAMIADKLRLEHSMVK"
     gene            25122..26243
                     /locus_tag="PC700_000598"
     CDS             25122..26243
                     /locus_tag="PC700_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2579922.1"
                     /translation="MRVEAPIKVDRKTKKLAKRVESGEIAVINHIDIDEVAANSLVEA
                     KIKLVINAAPSISGRYPNKGPGILTENNILIIDNVGEELFEELKEGETIEVVDGKIYR
                     KGKFLGAGEVLDKYEVSHQIKAAYENLAVELDRFIDNTIDYAKKEKGFILGEVEIPKV
                     KTNYANKHVLIVVRGQDYKEDLSTMLSYIEEVKPVLVGVDGGADALIEFGYTPDVIVG
                     DMDSVSDEALKKASEIVVHAYTDGRAPGLKRVEELGLDAVVFPAPGTSEDIAMLIAYE
                     YKAELIVAVGTHSNMIDFLEKGRKGMASTFLVRLKIGSKLIDAKGVNLLYRSKLKIKY
                     IWALIATALFPVLVVASLSPGVQQFIQLMQLKFRVLLQM"
     gene            26261..27136
                     /locus_tag="PC700_000599"
     CDS             26261..27136
                     /locus_tag="PC700_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HDF2579923.1"
                     /translation="MHINMKYYIVTIGAIFIALGIGMLVGFNLNNNQQLSEQQANIIN
                     DLDDKFNILKEKNDKLDSDLASVNRDYEEAVNFINKNVDKILAGSLNGKSIGIISANE
                     NDDYTKNIEDIINKSNGSIAFNIVLKENITNPEKLKEMSTKLGTDVKNANDAVNYIID
                     TLKKEDASDILTYLQELDVIKFNSLGNTYLKYDSVVIAGGNDAKDSTKQFEKIEKFVV
                     SKLKSENKYLVEVQNTGVKTSYVELYSKNKVATIDNIDEGIGSISLAILLQQGNIVGN
                     FGRLDTATSLLPSIK"
     gene            27160..27852
                     /locus_tag="PC700_000600"
     CDS             27160..27852
                     /locus_tag="PC700_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2579924.1"
                     /translation="MDSYISIIIPAYNEESRIKYTLESILDIKQINEIIVVDDGSIDN
                     TAKVVSELENEKIKFFKLDKNRGKGYALNYGIKLAMKNADIIGFLDGDLGSSAKEVEK
                     LIVPILNGEADVTIAKFPPAKRKGGLGFVKGLAKNSVFEMTGVELDATLSGQRIFKRE
                     VLEKFDEIPFGYGVEVGMTIDILKYGFKIKEVLVNMTHNETGRNLKGFIHRGKQYYHI
                     KKVLGQKKKEWR"
     gene            27860..28684
                     /locus_tag="PC700_000601"
     CDS             27860..28684
                     /locus_tag="PC700_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDF2579925.1"
                     /translation="MKDYILYTILLLTGLFGTYAVIPIFKNLLINGNVLRPNYKKDMI
                     PVSMGIVFLPMIVINGIILAFFTTEFKDLAYIFMFMFGMISMFFAGILDDVIGNRDVS
                     GLKGHFKSLFKGSLTTGGFKALFGGFVGLIVSVAISKDIIDIIVNTLIIALSTNLMNL
                     LDLRPGRAIKAYLFIMVIIFLTLTGFVQVLPLLIVPNVLAYFNYDLKARAMMGDTGSN
                     VLGISIGMLISFGYPFNIRLGWLIFVVIMHIFTEKYSLTKIIEKNKLLNFIDKLGR"
     gene            28689..29756
                     /locus_tag="PC700_000602"
     CDS             28689..29756
                     /locus_tag="PC700_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HDF2579926.1"
                     /translation="MISKRVGIVESIVSQTETLDDIRVNINGKIQRAYNYPKISGTIN
                     IGDEVVLNTTAVELSLGTGGYHFVITNLNNIESTLTEGGHIMKLRYTPLQIKVDSVEE
                     QESIYHDKFANFKGLEGLSVVVGTLHSMLTPFAASFKRNNPNKKLVYIMTDGASLPIY
                     LSKNVDTLKEKKLIDSTITIGNAFGGDYECINIYTALITAKEILKADAVFVSMGPGIA
                     GTGTKYGFTGIEQGSILDAVKKLEGRAIAIPRISFADKRERHQGISHHSMTVFKEIVN
                     VNVDIPITIYDDEKLNFIKEQINLNGLDKKHNIIYIDNNKTKEDLDYFNLKVKSMGRN
                     YEQDEAFFKAASTAAYYLMEV"
     gene            29758..30294
                     /locus_tag="PC700_000603"
     CDS             29758..30294
                     /locus_tag="PC700_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF2579927.1"
                     /translation="MVLEEKTISSDRVYTGKVITLKVDTVEIPGQGYQKRELVEVGGA
                     VGIVAITDDNKVVLVKQFRKPIEKPIFEIPAGKLEKNESPKECAERELKEETGYSAKN
                     IKLIHKFFTSAGFSNEIMFVYLATGLTPGENNLDADEFLDVYEIELEEAYNMVLKNDV
                     EDAKTSIGLLLVKDMFKN"
     gene            30362..30967
                     /locus_tag="PC700_000604"
     CDS             30362..30967
                     /locus_tag="PC700_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HDF2579928.1"
                     /translation="MRRTYRKNDFRELNRQIIIIGLLFMMSIVVGSYINKILPGSSNN
                     ILNNINPAVEYYNLNISIKDTVIQNLKSDAIFMGSIALLSLFVVTIPAVLVAFVLKGM
                     SIGYTINSCILALKFKSIKMILIILFKNLIIIPCAIILALISLSYFKEMVYEFKKKNR
                     KNMQFLIKRYILNIIIIIALSLGLQLILNTVSIGIIKFLAK"
     gene            30979..31893
                     /locus_tag="PC700_000605"
     CDS             30979..31893
                     /locus_tag="PC700_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2579929.1"
                     /translation="MDIIEGYIDYIKDKKKLSENTVASYFMDIKKYMEYINQKSIKLV
                     DIVENDIISYLIKLEKDNVSIATIARMISSIKSFHDYLFLNHICTNNPAKDIKKPKIK
                     KENINILTEEEIERLLNFPKLTTPKLIRDKAIFEVLYGTGIKVSELVEMNIEDIDLDI
                     DYIYCNSCCKNQRVIPLCDITKLYLEKYLKEARPKMAVEGEKSLFVSSLGQRFTRQGL
                     WKVIKKYSNLANIDKNINPTMLRHSFAIHLLNEGANIAVVSKILGNVNLSSLQVYLNH
                     IDKNVRREIKEKHPRNDVDVELQKAEAK"
     gene            31935..33107
                     /locus_tag="PC700_000606"
     CDS             31935..33107
                     /locus_tag="PC700_000606"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDF2579930.1"
                     /translation="MSRVIWIVIDSVGIGALPDAEKFGDSKDVSTLGNIFKEYPDIQI
                     PNMRNLGIGNIDGIDFFESIKEPIGCFGKCKEMSQGKDTTTGHWEMTGIIVDKPFKTF
                     EHGFSKEIIEEFEKKTGRKVVGNKPASGTVIIDEYGEHQIKTGDVIVYTSADSVFQIA
                     ANEEVIPLEELYNMCKIAREIMMGDNAVARVIARPFIGKKKGEFVRTSNRRDYSLDPF
                     EPTVLDNIKESGLDVLAVGKIEDIFNGKGITDAIHTKSNMDGVDETLNYMKQDNKGLI
                     YSNLVDFDSKYGHRRDPEGYKKALEEFDSRLPEIMANMREDDILIINADHGNDPTYKG
                     TDHTREYIPVMIYGNKIKKGFNLGVKDTFADIGATVADILNVKLPKHGSSFKEDLF"
     gene            33154..33966
                     /locus_tag="PC700_000607"
     CDS             33154..33966
                     /locus_tag="PC700_000607"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDF2579931.1"
                     /translation="MFEKIAQSSKFINSKSNIKPKIGLILGSGLGDLANDIEDPVTIK
                     YSEIPNFPVSTVAGHAGQLVIGKLEGKEVIAMQGRFHYYEGYSQKEATFPVRVMKELG
                     VEILIVTNAAGGVNKEFKAGDLMLIRDHINFSGSNPLVGKNDDRFGARFPDMSDAYSS
                     KYVDVVKNCAKECNIDVKEGVYMFFSGPNYETPAEVRMAQILGADAVGMSTVPEVIVA
                     SHSNIGVIGISCITNMAAGILNQPLSHEEVIETTQKVKSEFMNLVKTTVKNL"
     gene            34170..35495
                     /locus_tag="PC700_000608"
     CDS             34170..35495
                     /locus_tag="PC700_000608"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HDF2579932.1"
                     /translation="MRMYDIIKKKRDNLELSKKEINYFIENYSKGDIPDYQASALLMS
                     IYLNKMNKQETVHLTEAMMNSGDMINLSEVDGIKVDKHSTGGVGDKTTIALIPLVASC
                     GAPVAKMSGRGLGHTGGTIDKLESIPGFSTEMEISKFIDSVNKSKIAVCGQSAKVAIA
                     DKKIYALRDVTATVDNISLISSSVMSKKLASGADAIVLDVKTGDGAFMKTLEESFELA
                     KSMVDIGTGMNRDTIGIVTDMDEPLGFAVGNSLEVIEAIETLKGNGPKDLVELCEVLG
                     AYMLVLSKVAHDFEDGVEKIRESIKSGTAIQKLKEFIENQGGNKDVVDDYSLFKKSKY
                     KYPVKSIKSGYISKIKAEDVGVSAMILGAGRETKDDILDLSAGIILEKKVGDYVNEGE
                     VLAYMYYNDEQKLSLAKGRFIDSYSIVDSKIEKNKLIYGIVTKEEIKKF"
BASE COUNT        14394 a         3550 c         6548 g        11087 t
ORIGIN      
        1 gtaataggtt aagttagaat aacccattac ctatttttat ttgtaagaaa agtgtacata
       61 gagttaaaag taaaaaatac aactaataaa actattgaaa acactagata aataataaat
      121 aaaaataatt ataaattttg gcatatatat tgctaatcat agagataaga cgacaaaata
      181 attgggaggt ttaatgatga aatacaaaac aacagggact tgtgctacag aaattgaatt
      241 tgaagtaaaa gaaaataaag ttacaaatgt aaactttata ggtggatgtg atggaaattt
      301 aaaagggcta aaagtattag ttgaaggcat gaatatagaa gatgtaatac aaaagttaaa
      361 aggaatagag tgtaaaacaa agccaacatc ttgcccagac caactgtctc tagcattgga
      421 aaattatatg aatataaagt aatacataaa acaatcgatt agttaggagt ttaattatga
      481 aaccaataat agggatttta ggaaatttaa taataatgga aaatggtatg tttccaggtc
      541 ttgaaaggtc atatgtaaat aatgattata taaatgcagt gctaaaaggt ggcggtagtc
      601 cagtaataat tcctgtaaat acagataaag aagttataaa aaaacagatt gaaatggttg
      661 atggagtctt aatatctggg ggatgggata tcaatcctca actgtatgga gaagaaacaa
      721 gagaagaaac gacatttata tatccagaag tggatgaatt tgatttaatt gcaataagta
      781 tagcattaga acttaaaaag cctatattag gtgtatgtag gggactacaa attttaaatg
      841 taagtttagg aggaacttta tatcaagata ataatcttat agaaggaagt tatataaaac
      901 atacacaatc ttctaaaaga catgtagcta ctcataaagt cgatgttaaa gaaggaagta
      961 ttttagaggg aattttagga aaacaattac ttacaaatag ttatcatcat caaagtgtca
     1021 atcagcttgg aaaaggatta aaagccatag catactcaaa agatggtata atagaagcta
     1081 ttgagaaaga ggaggaaaat tttgtagtag gagtacagtg gcatccagaa atgatggtag
     1141 attactgtga taaaatggaa aaactattta aatattttat aaatatttgt tcaaatttat
     1201 aatatacctt ggggggattg aacatgaacg aaaagaacaa aatgggatta ataagtatta
     1261 tacttttagg tattaatgca gtagtaggag ctggagtttt tctattacca ggagatgcaa
     1321 tgaaatcatt tggtgtagca agtatatttg tatatatatt tgatatgttg ttagttttat
     1381 ctatggcatt ctgttttgct gaggttgcag gtaaattcaa taaaaatggc gctgcctatg
     1441 tatataccaa agaagctttt ggagattttt gtggttttga agtaggtctt atgaaatggg
     1501 taataggatg tatttcttgg ggagctttaa tagtaggttt tcctacatca ttatcagcag
     1561 tatgggctcc agcaggtgaa ccacatatac aaaaaattat aattgtagcc atgatagttg
     1621 ggcttacaat tataaactta ttaggtgtat ctctatcaaa aattgtacaa aatgttataa
     1681 ctgtaggtaa actaattcca ttaatactat ttataggaat aggtatattt tttataaaag
     1741 gagttaattt tacttcttca acaatggtac ctccaggagc tggagcaaca gagtttggag
     1801 cagcagcact tttaatgttt tattcattta caggatttga atctatagca gttgcagcag
     1861 aagatatgga aaatccacaa aaaaatatac caatagcaat tataagtgta atagtaattg
     1921 catcaataat atatatatta aatcaagttg tatgtgttgg tatactagga gattcgttat
     1981 ctagtacatc tacaccagtg gcagatgctg caagaatatg ctttggaaat atgggagctg
     2041 gattggttac ctttggaaca ttggtttctg ttggtggaat ttgtatgtgt ggtgcttttg
     2101 taaatccaag aagttgtgta gctttagctg atgataaaat gttaccaaga atatttgcaa
     2161 gaaaagataa aaaaggtaca ccatatgtag cgataatagc tactatgtta atcactatac
     2221 caatagctct atctggaagt tttgctgaat tagcagcaat aagtgcagtt gctagattca
     2281 tacaatacat accaacatct ttatccgtat tagtgtttag aaagaaaaga ccagaattag
     2341 taggtacttt taaaactcct tttggagcag tgattcctct aattgctgta tgtgttggaa
     2401 catggttgtt attccaagca tctatgcatc aattaattat gggattagga gctttggcaa
     2461 ttggagtacc attatacttt ataatgaagt cttataatag aaaagtctgt ggtgaaaatt
     2521 taaagaaaat agtttaatta tgttataaga atttattgag ttaaaattag acattggcag
     2581 aaaattaaat acaaataagt atctataagg ctatattaaa atattattaa ttttttattt
     2641 taatatagcc ttatattgtt atcaataaat gaaaaatatt gtaacaacag aagaacaata
     2701 gtattatgtt tatagataaa aaacttatta ggaggtaaat ttatgccaac aataacatta
     2761 aaaaatggtg tagacgtact tattagagaa ggtgtaagag aagatgctta aagcattata
     2821 gatttttata atgaagtggg tggagaaact cattttctgt catttggaaa ggatgaatat
     2881 aaaatatctt tggaggaaca agaaaatgct atagaatcag caaaagcatc tgataattct
     2941 gttaaactga ttgcatttat agatgatgaa atagttggga ttgcaacgat tgattctaat
     3001 caaaaagcaa aaggaaagca tgttggagta ttaggaattg ttgtaaaaga aaaatattgg
     3061 ggaattggat tgggtaaaag gcttatgtta gaccttatag aatggtgtaa atcaaatgga
     3121 ataaccaaga agataactct tgtcactaat gaagaaaatt ataatgctat aggattatac
     3181 aaaaaagttg gatttgaagt agaaagcata ctaaaaaaag aatgttatta taatggtgtt
     3241 tatactgatt taataggaat gtcactttta ttgggaatat aattagtgta cattttatct
     3301 cgaaaaaagt atattaagta tggtataatt aaaaaagaat ataacttaat ataattttgg
     3361 aggacgaatt atgaaaaatc tgtgtaaaat atcagatgat tatttaaaca gcaagcttaa
     3421 gtatttaaag ttattatcaa aacaatatcc aagtatatca aaagcaagta cagaaataat
     3481 aaatttagag gctatattaa atcttccaaa gggaacagag cattttataa cagatgtaca
     3541 tggtgaatat gaaccatttg tacatgtatt aaaaaatggt tctggtgtaa taaagagaaa
     3601 aatagaagaa ttgttttcaa atacaataag agacagcgaa aaaaaaatgt tagcaactct
     3661 tgtatattat cctgaacaaa agttagacct aataattaag caagaggaaa atatagatga
     3721 tttttataga ataaatattt atagacttat agaactttgt aaatatgctt ctagcaaata
     3781 tactaggtct aaagttagaa aactgttacc agaaaatttt aaatacataa tagaagaact
     3841 tttgcatgag catgttaaaa gtgaacataa agaagagtat tataaaagta tagttgaaac
     3901 aatagttgac ataggaatag ctaaggagtt tataattgct atttctacag ttatacaaaa
     3961 attggttgta gatagacttc atgtaatagg agatatttat gatagaggtc caagaccaga
     4021 tataattgtc gataagctta ttgaacatca ttgtgtggat attcaatggg gaaatcatga
     4081 tatattgtgg atgggtgcag cctcaggtga aaaaacttgt atagcaaatg cactgaggat
     4141 atcagctaga tatgcgaatt tagacattgt agaagacata tatggaataa atctgttgcc
     4201 tttagccact tttgctatag aaatgtataa agatgaccct tgtaaagaat ttattccaaa
     4261 ggttaatgac caaagtgtaa caacaacaga aaagtcttta atggcaaaaa tgcacaaagc
     4321 aataagtata attcaattta agcttgaagg tgaggttata agacgaagac ctgaatttga
     4381 aatggaacat agacttttgc ttaatatgat aaactatgat gaaggtacga ttactttaaa
     4441 aggtaaaaca tataaattaa aagatactta tttacctaca atagacaaaa aagacccata
     4501 taaattaact atggaagaaa gaaatgttat tgataaatta gtatcttcat ttagaggtag
     4561 tgaaaaatta caaaaacatg tttcattttt gttttctaaa ggaagtatat atttaaaggc
     4621 taactcaaat ttactgattc atggatgtgt accattaaat gaagatggaa gctttatgtc
     4681 tatgaatata atgggaaaag agtataaagg taaggctctt atggataaga tggagtctct
     4741 agcaagagaa ggatttttct ttaaagataa ggcagaagaa aaactatatg gtatggatat
     4801 tatgtggtat ttatggactg ggaaatgttc atcattattt ggtaaagatg atatgactac
     4861 atttgaaaga tactttatag aagaaaaaga gactcataaa gaaaataaga atccatattt
     4921 taaacttaga gaaaatgaga tggcttgtaa aagattattt gaagaatttg atttagagct
     4981 tgatgaatcc catataataa atggtcatgt tcctgtggag agtaaaaatg gagagagccc
     5041 aataaaagct aatggtaaaa ttctcgttat agatggtggt ttttctaggg cttaccaaaa
     5101 aactactggt atagctggat acactttgat atacaattct agaactttgc aattagtatc
     5161 gcatgaacca tttaattctg ctgaagaagc cattgcaaat gaaagtgata tcttatcgac
     5221 aactgttgta gtagagcata aggcaaagag aaaaatggtg agagatactg atgaaggagt
     5281 aaaaatacaa gaggaaatag aagatttaaa actactttta atggcttata agaaagggct
     5341 aataaaagaa atgtagatat tatagaggca cttcataatt gaagtgtctt cttgttaata
     5401 cttattaaat atattataaa gattaataat agtagtttat attatactta ctaagtgaat
     5461 ccaagataaa tgggttcact ttttttattt ataaacatta ttaaaaaaat atagatgttt
     5521 tggttatatt ccttgtcctt tataaataga attatataaa caaaggagga ggacaagaga
     5581 tgaataaact ttctgatgaa ataataaact ctctttctac aactataaga gaaaaggtag
     5641 aaaaagtctc aaacaataat ctgaacatag aagaaattag gttacgctca caaaaaccat
     5701 tgatactaaa tgcaaactct aaagattatt tttacaatca aaaaactatg acacttgatt
     5761 taaatcaaca aaactcatat gtagtaacaa gagaagatgt agagcaaact tttcaaataa
     5821 tatgtaaata ttcaatacat tcatttatgg atgacataaa aaaaggattc ataactttga
     5881 gaggaggtca tagagtaggg ttagttggaa aagcaatagt agaagatgga caagtcaaaa
     5941 atataaagca tatatcatct ttaaatataa gggtatcaag ggaaattatt ggatgctcgg
     6001 ataagatttt aagtcatatt ataaagggaa aaaatcagat taataatact ttgataatat
     6061 caccacctca atgtggaaaa acaactttaa taagagatat agtaagaaat ttaagtaatg
     6121 gtaatgaaga ttatggattt aaaggtttaa aagtagcttt ggtagatgag cgtaatgaga
     6181 tagcaggagc ttatctaggt gttccacaaa tggatgtggg aataagaact gatataatag
     6241 aaacttgccc aaaagatttg ggtattacga tgcttttaag gtctatgtca ccaaatgtta
     6301 ttgtgacaga tgaaattggg agtgaaaaag aaataaaggc attatacaca gcattaaatg
     6361 gaggtatagg attaataact actgtgcatg gagattcaat agatgatata caaaatagaa
     6421 aagaattaaa tagacttctg gataaagagt tatttaaaaa ggttataatt ctttctgcaa
     6481 aaagaggagc aggaacgatt gaaaaaattt atgatttaga agaaaagaga tggtattttg
     6541 caaattaaaa taattattat agctctcttg gtaggaagta gttatttgat aggtgaacaa
     6601 atatacaaaa cttatactcg tagacataaa caattaaacg atttaataag agttttagaa
     6661 attttgagga tggatttatc tttcggactt tatacattag aagaaatctt taataggata
     6721 ggtggaaata aagagttttg tttttggaaa tttttctatc aaatatcaga aggacttcac
     6781 aatgaacaaa gtaaaacttt ggaaataata atatcagaaa atattgatgt cttaagtaaa
     6841 gaaacttatc taggcaataa agaaattgag gaactgaaaa atctaatact tactttaggg
     6901 aaaagcgata tagagtcaca acaaagaatg atagatttat ctatagaaaa tttgaaaaaa
     6961 caaacctatg agacaaagga agatataaac aaaaaaggcg tattgtataa aaaattagtg
     7021 acattcatag gtataggtat atgtattata ttgatttaat atggggaggt ttattatgga
     7081 aatatcattg atattaaagg ttgcaggtgt cggtattcta atatcagtat tgaatatgat
     7141 attggaaaaa acagatagaa aagattgggc aggtcttact acattagcag gtgtaataat
     7201 cgtattgggt atggtaataa cagaaataag tgacttgttt aatactgtaa gaacaatgtt
     7261 tcaactttac taaaaaggag ggagtaattt ggaaataatg caattaatag gaattgcaat
     7321 catttcaact acactctgtt tagtaattaa aaaagataga ccagagatag caaatttcat
     7381 agcaataata actggagtaa taatactatt gtcagttatg tttaagctaa attttatagt
     7441 agatagtata caagatttgg ctaacaaggc taacataccg actatgtata tatcattaat
     7501 aataaaatta attgggatag catacttaat ggaatttgcg atacaacttt gtaaagattg
     7561 tggggaagga aacattgctt caaaactaga atttggaggt aaaataatag ttatgtcaat
     7621 gtctttccct atacttttgt ccattgtaga aatggtggtg aatataattc catagatgtg
     7681 tatcttggaa tgaatataag tatattagag aactttcgtt gtattgaggt ttatagagtg
     7741 aagagaggca tggttgctta tgaaaaaaag atttttacca atgattatag gttttttatt
     7801 tactttcttt tttgtaaata tctttgctat tatgatattt gctaatgaag taccaagtag
     7861 tgaagataaa gaatatggtg aaacaaaaaa tagcatagat aaatatatag atggtcaatt
     7921 agacaagcta gatataaatg agattcaaga ctatataaat aaagaaatag ttattaatga
     7981 tgttaattta aaatcatttg taaaagattt aattagtgga gaaaaaaata tcttagattt
     8041 attcaataaa gatggattga agatattgat gtttgatgaa tttaaagcaa gtttaaaggt
     8101 agtagcagta attttggtac tggctctgtt atcatctatt ttaaagagtc tggaaaactc
     8161 tttttcatca ggtgcagtaa gtcaaatagc aacatatata atatttatca caatggtatc
     8221 acttacactt attggtttta aagatgtatt acagatttgt tatgatgcaa tagaccatac
     8281 tgtaggtctt atgcaagtta taatgccaat attaataaca tttttacttt taattgggtt
     8341 cccaataacg tcgactactt tgaatcctat cttcatagga ggagtaacat ttataaatgt
     8401 attttttaaa aattttctat ttgtatctat tactgttgca tttggaattt taataataaa
     8461 caatttatca aaaaatataa ggctaaagag atttttctcc tttgtaaaac aaataaatta
     8521 tgtttctatt ggagctatgt ttactgttta cttaggtctt gtatccatac aaggattgta
     8581 tgtaaccagt tttgataaat tcagtgtaaa gactgctaaa tttgctatag gtaattttat
     8641 tcctgttgta ggtggatttg tatctgactc tgtggatatt ttattatctt catctcagct
     8701 tataaaaaat atatttggag gaatagggct tatcttactt gttggaattt gtttacttcc
     8761 agtaataaaa attttatcag taatagtagt gtacaaatta gcagcaataa ttgtagagcc
     8821 tgttggagag gatggaatat ctaatttttt aaatgaagtg gcaaatttaa tgataataat
     8881 gctagcatca gtaattgcta taacagtaat gttctttgta actgtagcta tcttgacctc
     8941 gataagtgtc gtaagtcaag ggtaatttat atatcagtga aaaataatta catttaatct
     9001 aatgttttta gagaggagga tgctatgtta gagggcataa aggcatggat agttagtgtt
     9061 ttgataggag catttatagt aaatatagta gatatgattt taccaagttc taagataaaa
     9121 ccttatgtca acctagtttt aaactttatg tttgtattta ttgtaataac tccagtagta
     9181 ggattttttt caaaagatat gagtttagaa gatagaatat taaaatctat gggaaactac
     9241 aacaagcagt atgtagatag tacaaatgct ttggcaaaag agactggaaa taatagtcta
     9301 tccaagggat atgaggatgg gttaaaagaa gtacttaaat taaaacttga tgaatatggt
     9361 tatgatttag aagatataga gcttaatggg gcaaatataa ataatataaa aataaaagaa
     9421 aagaataata gtgcaaaaag taataacagt tccaatgaag aaaataaaaa taatagtact
     9481 aaaagagagg aagaaaacat aaactctaat gacaaagaaa attctaaaca agtcttcaaa
     9541 aaaggtacag agtatgggtt gaacctaaat gaagaaaaat taaaaaacga cttaataaag
     9601 gtgctggatg tttctataga agatatacaa attgataaat aggagttggg agaatgttga
     9661 aaaacttaaa tgacaaagat aagagaaagg tatattcact aataactata gcaggaatat
     9721 gtgtggtttc attagtatta ttatcttgtt ttcctagtag caaaagtgat aaacaagttg
     9781 gaaaaacaga gactaataaa aatgctgaaa agcaagtcac taaaaaacaa gaaaaagatg
     9841 atttagagtc aaaattaaca gcaatactat ctaaaattga tggtgcagga gacgttgatg
     9901 taatggttac atttgaatca agtgaagaaa tacaacctgc ttttaattca aataatacaa
     9961 cagaaacaac agaggaaaaa gatgcacaag gtggagaaag aacagttacg acttctagtg
    10021 aaaataaaac aatgataact tcaaattcaa gtgaccctgt agttataaaa actacagagc
    10081 caaagataaa gggagtgata gtggtagcaa gtggagcaag tgacccaagt gttaaagaga
    10141 cactttatag tgcagtacaa acatcattac aagtagctgg tcatcaagta gaaatatatt
    10201 ctaaataaat acatataacc ggggggataa atgaatgaag tttaattata agggaagagg
    10261 atttgtaata ataactttaa ctgcaatgtt agtggtagta ggaacagtga attatcaact
    10321 aagtaagaaa tctttattag aaacatcaaa agagtttaag gcatatgaag aagcacagtt
    10381 gcaaaaaaat actgatgata gtgatagtag taagacagag gattctagta atgtagataa
    10441 acaaggtggt aaggaaagtg ctgatattga tatagtcgat agcaaagcta gtaaagtaaa
    10501 agaaaaggca actgagacaa gtaaggaaat aaaagctcaa ttatcatcag agaaaaatat
    10561 gaaaaaggca tcttatattt tagatatgaa aatgaataga gaaaaacaaa gaaatgagtt
    10621 agttcaagat ttaaatgaaa tgataaataa tccatctaca acagaagaat ctagaaaaga
    10681 agcttctaat atgaaattaa atatagttaa aattcaagaa aaagaacttc aaatagaaaa
    10741 ccttttgagt acaaaaggat atgaagaagc gttagtttat ataagcgata acaaagttaa
    10801 tgtagttgta aatgaagcta aattagaaaa gaaagatgca gcaaaaatat ttgatttagt
    10861 tgcagaacaa gccaatgtga agtatgaaaa tattaaactt acaaataata atagtaataa
    10921 gtaaagtata aacctcagaa ttaactgagg ttattttctt gcaaagtaga cttgtatttg
    10981 gtattattat acttataaaa atagttcctt aaaatcagga ggtaagtttt aatatggaag
    11041 ataataaatt tggacaagta aaaatatcta atgatgtaat agctacaata gctggtttag
    11101 cagcattaga agttgaaggc atagaaacta cagcaacatt aacagataag ttgttaaaaa
    11161 ataatggagt aaaaatacaa atcgaagaag aggatgttaa tttagatgta atggttacga
    11221 taaagtatgg aatgtcaata ccagatacag cttttaaagt tcaagaaaat gtaaaaaata
    11281 ctgttgagac aatgacagga ctgaaggttt ctcaagtaaa tatacacatt caaggaatca
    11341 gctttaagaa ggataaagtt gataaagaag aagcaaaagc aactaagaaa aattaatatt
    11401 agccctctga aaagtcagag ggttttttgc tggtaattga ttttatatgg ataattaaac
    11461 ttaaaggaga attttgattg atgaaaaaag atagggcaca aaagagtaca acaagagaat
    11521 atataatgaa acttatatac caaataaata ttaataaaga agattttgaa actttagaag
    11581 ataaagtaga taatttttta aaagataatt cagaacatat tataaataga tataaagaac
    11641 ttgcacttca atactcaaaa aatacaaatt taaagttaga agatactgaa attgaagatg
    11701 ttatagacaa gaaatatata aatacagtat gcaaagcatt aaaagaaaat catgacaaaa
    11761 tagatgagtt aataaataag catgctaaaa actggacagt agatagaatg ccaaaggtag
    11821 atgtttctat acttagattg tctgtgtgtg aaatacttta tttagacact cctaataaag
    11881 tatcaataaa tgaagcggta gaacttgcaa aaatatattg tgatgataag tctcctaaat
    11941 ttataaatgg tatattggga agtgttgttg atgaaattgg gaaataatat aataattgga
    12001 attgatacta gctgctatac tacttctata gcagctatct ctttagataa gaaagttata
    12061 ttcaatgaaa aaataatgtt agaagtaagg gataattcaa aaggtttaag gcaaagtgaa
    12121 gctgtatttc aacatatcaa taatttaggg attcttagtg ataggataaa atcttttaaa
    12181 gataagttta atgtagaagg agtttgctca tcaaaaaaac cgagacctgt tgaaaactca
    12241 tatatgcctg tattcaatgt aggtcataat tttggaaaat tattgtctag tatatatggt
    12301 tgtaggtttt atgagactac tcatcaagag aatcatattg aagcaagttt attaaatagt
    12361 aaattaaaaa ataataataa atttatttct gttcatatgt ctggtggtac tacagagata
    12421 cttttaacaa gcaaacaaga tagtcatcac aatgtatgtg atactaattt aggtaaaatt
    12481 gccaaaataa gtattaaaaa agatgataaa agtaagttat acaataattt tggatataat
    12541 atagatataa ttggaggaag taaagatatt agctttggtc aactcataga tagagtagga
    12601 attaagcttg gatataaatt tccttcagga aagtatttgg atgaaaatgc tttaaattgt
    12661 aatcttaaaa tagaaagtgg tctgaaaact tctgtaagag atgggtatat gaatttatct
    12721 ggattagaaa atcaggtaaa taagattata aatgataatg gagataatac taatcaaaaa
    12781 gaatacatat ctaaattagt attagattca gtagttagaa atatgtttaa atctttagta
    12841 tatttatgtg aaacttataa tgtaaatgaa gtagtatttg ctggtggagt ttctgcaagt
    12901 aagtatattc ttagagaatt aagcatgaag ttaagaaaaa agcatataga agcttatttt
    12961 acagaaccac aatattcaac tgataatgct gtcgggtgtg ctataatagg attgaataac
    13021 tttttaggag aaagagtatg aaactaagag ctttagatat aagtgaagca aactcatata
    13081 ttaaaagaat tttaataaac gacccaatac tttctaatct taaagttaaa ggagaaattt
    13141 ctaattttaa ggttcatagt agtgggaatg tatatttatc acttaaagat gaaacatcaa
    13201 agttaaactg tgtaattttt aaaagtaatt ttaatcgcaa tttaaaatta gataatggtg
    13261 taaaaattat agctaatgga tatatatcag tctatgaaag agatggtgcg tatcaattat
    13321 acattaatga aattgaaata gagggaatag gaaatttaca catagagttt aatagattaa
    13381 aggaaaaact taataaagaa ggattatttg accctaaata caagatacct atacctaaaa
    13441 tgccaaactc aattggcgtt ataacatctc caacaggtgc tgttataagg gatataataa
    13501 atgtaataaa aagaagatat ccgaaagtta atataaagct ttatccagta atggttcaag
    13561 gagataaatc agctgaagaa atctgtgaag caattagatt tttcaatcat atgaagaatg
    13621 ttgatacact tatagttggt agaggtgggg gttcaataga agaactttgg tcttttaatg
    13681 atgaaatggt agcaagagag gtttttaatt ctcaaattcc gataatatca gcagttgggc
    13741 atgaaacgga ttttactatt tgtgactttg tgtctgatat gagagcccca acgccatcag
    13801 cagcagctga gattgctact ccatcattgg atgatataaa ctataaactt ggaaacataa
    13861 aaagtaggat gagtaagtct cttacaaatc aaatagagct agaccaatat aggttagaaa
    13921 cagtatttaa taagataaat aactatttag attcgtacac aataaaagat aaagttatac
    13981 aattagacaa aatatatgat aaaataattt ttggaataga aaataattta aaattagaag
    14041 atgaaaaatt agtaaaaatt ggagcattac tccataattt aagtccatta gctactatgg
    14101 atagaggtta tagtataact caaaaaaatg gaaaagtaat aaatagtata aaaggattaa
    14161 aaataaagga tagtatagat atagtattaa aagatggaaa tttagaatgt atgatagata
    14221 aaattgaaaa taaagaggga tagtatgaat ttaacctacg aagaagctta taaaagatta
    14281 gaaagtattt taagtgaatt agaatctaaa aatgctagct tggatgaatc tttaagccta
    14341 tatgaagaag gtataagcct ttataaacat tgtaataagc ttttagatga tgctaaattg
    14401 aaaataagta aatttaatca attaggaata gaagaagact ttaaaataga ggaggaataa
    14461 aattggaatt taaacaatgt ctaaaagaaa aagcaagttt tgtagaaaaa gtacttaaag
    14521 aatatatgcc taaagaagaa gggtatcaaa aaactgttat tgaagctatg aactatagtt
    14581 taagtgctgg aggtaaaaga ttaagaccaa tacttacatt ggaggcttgt aaaatagttg
    14641 gaggaaatga agatgaagct attccttttg ctattgcaat tgaaatgata catacatatt
    14701 cactaataca tgatgatttg ccagctcttg acaatgatga tttaagaaga ggaagaccaa
    14761 caaatcataa agtatatgga gaagctatgg ggattttagc aggagatgct ttgttaaact
    14821 atgcttttga agtaatgctt gcaggctcaa tcaataaaga aaatccagaa aagtatctaa
    14881 aagctataaa tgaaattgcc aaaggtgctg gtatatatgg aatgataggt ggtcaagtag
    14941 tagatgtaga aagcgaaaat aaacaaattg aaaaagaaaa attagattat atacatatga
    15001 ataaaactgc tgcaatgatg gtaggctgta tgagagcagg agctactata ggtggagcaa
    15061 attctgaaca aatggaagaa ataactaaat atgcaaaaaa tatcggatta tcattccaga
    15121 tagtagatga tatactagac atagtaggag atgaagctaa gttaggtaaa aaggttggaa
    15181 gtgatataga aaatcataag tcaacatacc catctctttt aggtttagat aaatctaaag
    15241 aaattgcaca taatttgatt gatgaagcaa agaaaagtat agaaaaatta tctgatgatg
    15301 tagatttttt aaagggattg gctgaatata taatagatag agaatactaa aatatacagc
    15361 taaaatcgag gagggaaaaa tggatttttt ttcggagatt tttaacaatg gggctttggg
    15421 aataagtctg attgcttgtt tcttagcaca atttataaaa atatttactg gtaaggaaaa
    15481 aagaattgag ttatcaagaa tacttatttc aggaggtatg cctagttccc atagttcttt
    15541 tgttacaagt ttagctacag tggtaggtat tgaaaaaggg tttaattcta ctgattttgc
    15601 tataattacc gtacttgcct taattataat gtatgatgca gcaggtgtta gacgagctgt
    15661 tggtaaacaa gctactatat taaatcaaat ggtggcagat atacagcatg gaaagcatat
    15721 agagcagaag aaattaaaag aattaatagg tcatacacca ctagaagttt ggtttggagc
    15781 tttacttgga atagtaacag ctttaatatt gatgtaaatt gaaaatttac agaataaagg
    15841 tgaggataat atgtataaat atttagataa agtaaattct ccaaaggata taaaaaatat
    15901 gagtatagaa gaaatggatt tacttgcaaa ggatataaga aagttcttag taaaatctgt
    15961 ttcaaagaca ggaggacact tagcttctaa tctaggagtg gttgagttaa cattagcact
    16021 tcataaagta tttgatagcc ctaaagataa gattgtatgg gatgtagggc atcaatctta
    16081 tgttcataaa atagttactg gaagaaaaga ttgttttgta tctttgagac agtttaatgg
    16141 actaagtggt tttccaaagg agaatgaaag tcctcatgat atttttgata caggacatag
    16201 cagtacatct atttctatag ctacaggtat tgcctgcgct agggatataa aaaaagaaaa
    16261 ctatagtgtt atatctgtga taggagatgg ttctataaca ggaggtatgg cacttgaagc
    16321 tttaaatcaa ttaggatata tagataccaa tatgatagta atccttaatg ataatgagat
    16381 gtccatagat aaaaatgttg gaggaatgtc taaatatctg tcaagtatta taagaaactc
    16441 aacagttgag aaaatgacag atgaagttga taaaatttta aatgttacac aaacaggtga
    16501 aattttatct aagactgcac atagatttaa agataaacta atgtatagct tttctcctca
    16561 ggactgttca ttttttgatt cattaggtat aagatattat ggacctatag atggccataa
    16621 tactaaagaa ctaatagata tattgagaaa agctaaacac aaaaaagggc ctgttctttt
    16681 acatgtaatt acgaaaaaag gaaaagggta cagatttgca gaagaacaac ctgataaata
    16741 tcatggagta tcaaagtttg atataaagac aggtgttaca tctgcaaaag ttaagtctat
    16801 gtcaattagt gttggagaaa aacttgttga tatggctaat aataatgaag atatagtagc
    16861 aataacagct gcaatgccat ctggtacagg gttaaacctg tttgaaagtg catatccaaa
    16921 aagatattat gatgttggta tagctgaaca acatgccaca ggatttgcag caggtcttgc
    16981 aaaaaatggt atgaaacctt actttgctgt gtactcatca tttttgcaaa gggcatatga
    17041 ccaagttatt catgatgtat gcataacaaa aaagccagtt acttttctta tagatagagc
    17101 tggattagtt ggaaatgatg gagaaactca tcatggtatg tttgatttaa gttacttaaa
    17161 ttctattcca aatattgtag taatggctcc aaaagacaca agagagatgg agcttatgat
    17221 ggatttgtca ttaaaattag attgtccact agcaattaga tacccaagag gaagtagtta
    17281 ttacctagat aagggagaat atggagagat tgtactgggt aaatatgagg tattagatga
    17341 cggtcaagat acagtcatac tttgtattgg aagtatggta aaacatgctt tagaggctaa
    17401 agaaatatta tcaagagaag gaataaatcc gacaatagtt aatgctagat tcttaaagcc
    17461 aattgatgaa ggtatgttaa aagcattatt aaaaaatcat aaaaatgtag ttactataga
    17521 ggataatatt gtaactggag gatttggaag tagaataaac aagtttatta tagataatga
    17581 atataacgta aatatactta atatagcaat accagaagaa tttgtaaaac atggcaatat
    17641 agatgaatta tatgattttg taggattatc gccaaaaagt attgctgata aaataaggaa
    17701 attagttatt gagtaaaaat ctaatgtaga tattgtaaat taaaatttat ataaaatata
    17761 gaatttatca attagaataa atatcaagtg tgttacttca agctacttta gttatcatta
    17821 aagtagcttt taacagaaag gtaattgtat gaaaaaaaga atagatttat tattagtaga
    17881 acaagggtac tttgaaagta gagaaagagc caaaaaagca ataatggctg gattggtttt
    17941 tgttgataat caaaggtgtg ataaggctgg tacagaagta aaagaagatt gtagtataga
    18001 agtaaaagga aatccaatac catatgtcag tagaggtggt ttaaagcttg aaaaagcaat
    18061 gaaaaacttt gatttgacta ttgatggaaa ggtatgtatg gatatagggg catccacagg
    18121 tggttttaca gattgtatgc tcaaaaatgg agcaataaaa gtattttcta tagatgttgg
    18181 ttatggtcaa cttgcatgga aattgagaca agatgatagg gttgtgtgta tggaaagaac
    18241 taatataaga aatgttacaa tagaatatac aaagcagttt gctgattttg cgtcaattga
    18301 tgtgtctttt atatcgttaa agttagtact tccaaaggca aaggagttag tacgtcatga
    18361 tggggaagtt gtcgctttaa ttaaacctca atttgaagca ggaagagaaa aagtaggtaa
    18421 aaaaggagta gttagagaaa aatctacaca tatagaagtt ataaaaatga tttctgattt
    18481 ctcagtcgaa aatggatttg agattttagg gttagatttt tctcctatta aaggtcctga
    18541 aggtaatata gaatatctaa ttcatttgag aaatggaaat gaaggatatg aatttgatgg
    18601 tgagacatac aataacaaaa tagtagaagt tgttgaagct tctcataatt tagacaaata
    18661 ggttaaaagg gggcttagat ttgatcctcg aattatatat gaaaaattgt gccttagttg
    18721 aagaactaag gttgaatatt gataaaaact taaatatact tacaggtgaa actggttcag
    18781 gaaaatctat aataatagat gcacttggac tttgtttagg tgataaatat gataggtctt
    18841 ttttaagaaa aggaacagat aagggacttg ttgaggctgt atttttttca gacaatagat
    18901 atcttaaaaa aatattagaa gaaaatgata taagtatgga agatgataat ttattggtaa
    18961 taactaggtt gatatactca gatggtaaga gtactgctag agtaaatgga aggactgtta
    19021 aagtgtctct tcttaaagaa attgcatcta cacttataga tatacatggt caacatcaaa
    19081 atcaagcttt gttcaataaa gatactcatt taaaatttct agaccttttt ggagaaaatg
    19141 aattggaaga gtttaaaatt gcttataaaa atgtgtacca taagtatagt gaagtaaaaa
    19201 aagctttaaa ttgcttgaca gaaaataaag atgagatgca aattcaacga gaaatagatt
    19261 tattaagatt tcaaataaat gaaattgaag ctgcaaatct aaataagaat gaatatgaag
    19321 atttattaaa acaaagagaa gtttatagaa atagtgaaaa gatatataat aatctaaatt
    19381 cgagttatag caagttacat aatggtgagt ataatgttat tgacctaata ggtcttgcat
    19441 ctaaggaact taatgacata tctaaatatg acagtgtttt aagtgaatat agtgatactg
    19501 tagaaagaat tatgtatgag ttacaagaca tatctggtga gataagaaat tacaaagata
    19561 atatagactt tgaaccttat gagctagagc agattgagct tagaatcgac gaaattaata
    19621 atttaagaag aaaatatgga gacagtatag atgatatatt tgagtattat ggaaaaatta
    19681 aagatagatt agatgaaata ttaaatagag atgaaagagt tgaacagtta aaaagcaaac
    19741 tgatgaatat agaagaagat ttaaaaataa aagccagtaa attgactaaa gcaagaatag
    19801 aagtagcaac tactttagaa aaaatacttc ttgatgaatt aaaaagctta aatatgaaaa
    19861 atgtaatgtt caaggttaat tttgaaaaag gttcatttac tttagatgga gtagatgata
    19921 ttgaatttat gatatctttt aacttaggag aagatattaa gcctatatat aaagttgcat
    19981 caggtggtga gatgtccaga tttatgttag cgtttaaaac catattggct gatatagatg
    20041 atattgatac attagtgttt gatgagatag atactggaat aagtgggata gcagcacaga
    20101 tagttggtga gaaattgagt gatatagcaa agaaaaaaca gataatatgt ataactcatt
    20161 tacctcaaat tgctgcaaat gcagacactc attattgtat agagaaagat acttcaaata
    20221 ataggacgtt taccaatgtt agtaagttaa atgaatctca acgaaaaaat gaaattgcca
    20281 gacttatagc aggtaacaat ataacagaaa aaactattga acatgcaagt gaaatcattg
    20341 aattggctaa aaaatgttag tgaaaaacaa gcattttaat aggtgtaaag aatttttata
    20401 gaaatgcttt acacctattt tttatgatga aattatttta aaatatctag tgcctatatt
    20461 gaagaaaagt aggtttgaaa atggaatgaa ctttgtataa atttgtaact ttgaaatttc
    20521 taaatgctta agtataatat tatttattaa ggtagtagtg aggggaggca taaaatgaat
    20581 aaaaaagata ctatgcactt aatttttata attgtaattt ttattttatt atggtatatt
    20641 gtggtagcag taagtgagat taataaaaat attgaaagtt gtagtaacaa attagattat
    20701 ttgtcacaag agcttatgag tactaagtcg gatattagta atactataaa tgaagaaatg
    20761 tcaaaaagtt atataacaaa aagtattgac ttaaaagtaa aaaaaatcga gaaaaaagaa
    20821 tgtactattg atgtagatgt tcaattaagt aaacttggaa aaaatggaaa agtatttttt
    20881 atgtataaag aagatagtga taaatggaat gaaactgaga tgacaaaaca tggggaatta
    20941 tcatatgcat gtgaaataaa gataaataca ggaagtgaat atgattataa agtagttaca
    21001 agtggagata tatctgaaag ttctgatgta caaaaactga ctaaatctga ttatatgcct
    21061 gaacaaccta tatttaattc tgggataaga gataatagag aatactttat tgaaatagta
    21121 gaaaatagta atctttttaa tcatgagagt gaaaagtcaa aaaataaagc ttacgataat
    21181 atcaggttag aaaaagtaga tattattgta aatgaaggaa aaaaagacac tatatacaaa
    21241 gcccaattag ctgaaggctt agatgatggc aaaactaata attcaaatag aaatcaagtg
    21301 aattatgaag taagttttcc taaaagggat attgatgaca taatttatat taaagctaaa
    21361 cttacataca ataatggagt agaatataca aaggatataa ctgaagatat aactatggga
    21421 ttacaagatt tagaaaaata aatacatatt taaataaata ttcaaataga tttgttcaca
    21481 aatagagtat aaataaaact gtagtagttt tacttatact ctattaaaaa attataattt
    21541 taattccata tatctagccc cttcaattgg attataagta taaagttcta tatcataaaa
    21601 accaaacttt tcatataatc cttgtgcact tttcatgctt ggaagagtat ctaatctcat
    21661 gtgtgtatat ccaatttttt tagcttctac aatgatttct tccaatagta tttttcctat
    21721 ttttaatcct ctaaattgat ttcttacata taatcttttt aattcacata ttttaccttc
    21781 aagttttttt aaagcaacac aacctgccaa attttcatca acaaatgcca atatcaatga
    21841 gcctgaaggt tttttatatt ttcctggaag tgtttttaat tcattattaa agtcttgaaa
    21901 acataagtct atatttagag aatttgaata ttcagtaaat agaagtttta cgttttcaag
    21961 attttctaaa tctttttctt caacaaattg taaagttata ttttgagcca ttttatccgc
    22021 cattatataa acatgtttat ataatgactt cacctccttg atatatgtat atttttctta
    22081 atatcaatta gattttaata ccattacttt ttcaccacca tagtcaagct ctccattaaa
    22141 cttaaatcca aaatctttat aaagcttaat agccatttta ttgtcttcga atatactaag
    22201 atatagttca tcacagttat attcattttt aagatgattt acaagaaaat ctaatgatgc
    22261 ttttccacat cctttacctt gatattttga atcaatcata aatctatcaa gccaaactct
    22321 atcatcttct aaacgaccat acatagcaaa tcctattaaa gaattttcat catatattcc
    22381 aacaggtacc caagctgtta taaatttaga ttcagcaata gaaagtgcat tgctttctat
    22441 gtaatctaac tgacttttat ctacagacaa actaactaaa tcgtgccaat tatttatgtc
    22501 tgcaattctt acatttaatt tcaagatatt accgtccttc aaactttttg ttacaatcta
    22561 atactatcat attatgtatt aaaaatcact tattaagatt ttaaataaac tttttgaatg
    22621 aataaattct caggaaaaag gaattataaa tacataataa tacagcatag gagtagatgg
    22681 atatatgaac aaaataaata aaaatttcaa aaatatttgg ataatattag gagctgtatc
    22741 gatttttacc atggttataa tttttaataa aaattgtaat gttaataaag agactcaaat
    22801 agaagctttt acaattaaga atcatgataa aaaatttgta tatcctatgg gaaatataat
    22861 tggagtaaaa gcaaatacag atggtgtatt ggtcttagga tatgaggaag aggatgtagg
    22921 ctatattgga ggcatacaaa taggtgataa tatagtaaaa atagataata aaaggataaa
    22981 aaatagtcaa gatgtatcag agattttgaa taagattaaa aaaagtaaag ttgaagtgac
    23041 ttttgagaga aaaaataagt ataaaacaga agtaatagag actaaaaaag aaaatggcaa
    23101 gtataaactt ggattatggg taagagataa aatatctgga attggaacta tgacttttta
    23161 cgaccctagt atggaaaagt tcaaagcaat cggtcatgcc ataaaagatt cggatactaa
    23221 tgaactatta aagataaagc aaggctatat atataagcca gaacaattaa aaatagtaaa
    23281 agctagtaat gaaaaagtag gtaaaataaa aggagatttt aatgacagca atttaatggg
    23341 taatttctca aataattcag agctaggtat aagtggtaat attacagaaa accataataa
    23401 agagtttaat gtagcaaata aagaaaagca attaatagaa gttggaagac cgcaagatgt
    23461 aaaaatagga gatgcagtaa ttctttttga ggataaaaac aaaaatataa caagctatga
    23521 tataaaaata gaaagtatag tatatgataa aggaaattat agagatatgg taataaaagt
    23581 agtagatgac aagttattgg aatacacagg aggtatcgta caggggatga gtggagctcc
    23641 aataatacaa aataataaaa ttattggtgc aataactcat gtttttagag ataatccgaa
    23701 aaaaggatat ggtattttta tagatgaaat gataaaattg taggtgaggc attaaaaaat
    23761 tttattattt tatcaattat ctaggaggaa tataattttg gagtgtcgaa tatgctttag
    23821 agtagataat taggaagcaa ttgtgtaaaa agtttagttt tctgtaataa gaagatgttt
    23881 tttaatgggg ggatttttag tggaaaaaat caaaatagtt ttagcagatg acaataagga
    23941 tttttgtcag gtattaaaag agtatttgtc taatgaagat gatatcgata tattaggcat
    24001 agctaaggat ggaattgaag cattagactt agtaaaaaag acacaaccgg acttattaat
    24061 actagatgta ataatgccac atctagatgg attaggtgta attgaaaaat tgaatactat
    24121 ggatatacct aaaatgccaa aaataatagt actatcagca gtaggtcaag ataagataac
    24181 tcaaagcgca ataaatctag gagcagatta ctacatagta aagccatttg attttgttgt
    24241 gtttataaac agaattagag agctagtttc taatagagtt acacaagttg agccaaaacc
    24301 tagaccagtt caagagactc aaatgactag aagtgatttt gttaagaatg taggaaatat
    24361 agagacagaa atcacaaata taatacatga aataggagta ccagctcata taaaaggata
    24421 tttatattta agagaagcaa taaaaatggt tattgataat gttgagcttt taggtgcagt
    24481 aacaaaagag ttatatccaa gtatagctaa aaaattcaat acaacaccaa gtagagttga
    24541 aagagcaata agacatgcaa tagaggttgc atggagtaga ggaaaagttg acacaataaa
    24601 tcaattattt ggatatacgg tacacaatac taaaggaaaa ccaactaatt cagaatttat
    24661 agcaatgatt gctgataaat taagactaga acatagtatg gttaaataaa caagacataa
    24721 aaagtaaggc tttttaatta aggcattggc tataaatgcg tattacaagc agcgaaacgg
    24781 tataaccact agggttatac cgtttcgcta ttttaataaa tataaaaatt ttctttatta
    24841 tttgcttact atatcaatat aataatttta ttatactatg gatatagtat gtgtctttac
    24901 aagttgtaaa ctgacagtgg tttatttttt aatataaata ttgactttga tgcaggtaaa
    24961 ctttgtattt ttaagcgtat tatggaatat gttaaataaa aaaatgatga aatatagtat
    25021 tgtaaatgcc aaagatgcaa aacaaactta aaacatttat tttattgtta agtaatgcta
    25081 taatataatg tgattttaat aatgatagtg gaggtttaaa tatgagagtc gaggccccta
    25141 taaaagtaga tcgaaaaacc aaaaaacttg ctaaaagagt tgaaagtggg gaaatagcag
    25201 ttataaatca tatagacata gatgaagttg ctgcaaactc tttagtagaa gctaaaataa
    25261 aacttgtcat aaatgcggct ccttctataa gtggtaggta tcccaataaa ggtccaggga
    25321 tattaactga aaataatata ctaataattg ataatgttgg agaagagtta tttgaagaat
    25381 taaaagaagg agaaactata gaagttgtag atggaaagat atatagaaaa ggaaaatttt
    25441 taggtgcagg agaagtttta gacaagtatg aggtttcaca ccaaataaag gctgcctatg
    25501 agaatttagc tgtagaattg gatagattta tagataatac tatagattat gctaaaaaag
    25561 aaaaaggatt tatacttgga gaagtagaaa ttccaaaagt gaaaactaat tatgctaata
    25621 aacatgtatt gatagttgta agaggtcagg actacaaaga agatttaagt actatgcttt
    25681 cttatataga agaggtaaag ccagtattgg ttggtgttga tggaggagca gacgctttaa
    25741 ttgagtttgg atatactcca gatgtgattg taggtgatat ggatagtgta agtgatgaag
    25801 ctttaaaaaa ggctagtgaa atagtagtac atgcttatac agatggaaga gcaccaggtc
    25861 ttaagagggt tgaggaatta ggcttagatg cagttgtatt tcctgcgcca ggaactagtg
    25921 aagatatagc tatgcttatc gcctatgagt ataaggctga gttaatagta gcagttggta
    25981 cacattcaaa tatgattgat tttcttgaaa aaggaagaaa aggtatggca agtacttttt
    26041 tagttagact aaaaataggt tcaaagctta tagatgctaa aggcgttaat ttgttataca
    26101 gaagtaaatt aaaaataaag tacatatggg cattaatagc aacagcatta tttcctgtgt
    26161 tggtagttgc atcattgtca ccaggagttc aacagttcat acaactaatg caattaaagt
    26221 ttagagtatt attacagatg taaattttag gaggataaat atgcatatta atatgaaata
    26281 ttatatagtc actatagggg ctatatttat agctttgggt ataggcatgt tagtaggatt
    26341 taatttaaat aataatcaac aattaagtga acaacaagca aacataataa atgatttgga
    26401 tgataagttt aatatcttaa aagaaaaaaa tgataaactt gacagtgact tagcaagtgt
    26461 aaatagagat tatgaagagg cagttaattt tataaataaa aatgtagata agattttagc
    26521 tggttcgctg aatggtaaaa gtataggaat aatatcggct aatgaaaatg acgattatac
    26581 aaaaaatata gaagacataa taaataaatc taatggaagt attgcattta atatagtatt
    26641 aaaagaaaat ataacaaatc cagaaaagtt aaaggaaatg tcaactaaac taggtacaga
    26701 tgtaaaaaat gcaaatgatg cagttaatta tataattgac acccttaaaa aggaagatgc
    26761 aagtgacatt ttaacatatc ttcaagaact agatgtaata aagtttaact ctcttggaaa
    26821 tacatatcta aaatatgatt ctgttgtaat agctggtgga aatgatgcaa aagacagtac
    26881 aaaacagttt gaaaaaatag aaaaatttgt agtttcaaaa cttaaatcag agaataaata
    26941 tttagtagaa gttcaaaata caggagttaa aacttcttat gtagaactat attctaaaaa
    27001 caaagttgca actatagaca atattgatga aggtatagga agtatatctc tagctattct
    27061 tctacaacaa ggtaatatag ttggtaattt tggtagattg gatactgcaa cttcattgtt
    27121 gccatctatt aaatagaaat aattttaagg agagaaaaaa tggattctta tataagtata
    27181 ataataccag cttataatga agaaagtaga ataaaatata cattagaaag catattggac
    27241 attaagcaaa taaatgaaat aatagttgtg gatgatggtt caattgacaa tactgctaag
    27301 gttgtatctg agctagaaaa tgaaaagatt aaatttttta agttagataa aaatagaggt
    27361 aaaggatatg ctttaaatta tggtatcaaa ttagcaatga aaaatgcaga tattataggt
    27421 tttctggatg gagatttagg aagctctgct aaagaagttg aaaaattaat agtacctata
    27481 ttaaatggtg aggcggatgt tacaatagcc aagtttcctc ctgcaaagag aaaaggaggt
    27541 ttgggatttg taaaaggttt agctaaaaat tctgtgtttg agatgactgg tgttgagtta
    27601 gatgcaactt tatcaggtca aagaatattt aaaagagaag ttttagaaaa gtttgatgaa
    27661 ataccttttg gatatggagt agaagttgga atgaccattg atatattaaa gtatggattt
    27721 aaaattaaag aagtattagt aaatatgact cataatgaaa caggaagaaa tttaaaaggt
    27781 tttattcata gagggaaaca gtattatcac ataaaaaagg ttttaggaca aaaaaagaaa
    27841 gaatggaggt aatatcttag tgaaagatta tatactttat accattttat tattaactgg
    27901 attatttgga acctatgctg taattccaat atttaaaaat ttattgataa atggaaatgt
    27961 acttagacca aactataaaa aagatatgat accagtaagt atgggaattg tatttttgcc
    28021 tatgattgta atcaatggaa taatacttgc tttctttact acagaattta aggatttagc
    28081 gtatatattt atgtttatgt ttggtatgat atccatgttt tttgcaggga tactagatga
    28141 tgtcattgga aatagagatg taagtggttt aaaggggcat tttaaaagtt tatttaaagg
    28201 aagtcttaca acaggaggtt ttaaagcttt atttggtgga tttgtagggc ttatagtttc
    28261 tgttgctata tcaaaagaca taattgatat aatagtaaat acactaataa tagctctatc
    28321 aactaattta atgaatcttt tggatttaag accaggaagg gcaataaaag catatttatt
    28381 cataatggta attatatttt taacattgac aggatttgtt caagttcttc cattacttat
    28441 agtaccaaat gtacttgcat actttaatta tgatttaaaa gcaagggcta tgatgggaga
    28501 tactggttca aacgtccttg gaatatctat tggaatgctc atttcttttg ggtatccatt
    28561 taacattaga ttaggatggt taatatttgt agttattatg catatattca ctgagaaata
    28621 ctcattaact aagataatag aaaaaaataa actattaaat tttatagata aattgggaag
    28681 gtagtattat gataagcaaa agagtgggga ttgttgaatc aatagtaagt caaactgaaa
    28741 ctctagatga tataagagta aatataaatg gaaaaataca aagggcatat aattatccca
    28801 aaatatctgg aactataaat ataggtgatg aagtggtttt aaatacaaca gcagtagaac
    28861 tgagcttagg tacaggtgga taccattttg taataactaa tttaaataat atagaatcta
    28921 ctttaactga aggtgggcat ataatgaaac ttagatatac acctcttcaa ataaaagtgg
    28981 attctgttga ggaacaagaa agtatttatc atgataaatt tgcgaatttt aaaggattag
    29041 aaggtctttc tgttgtagta gggactcttc atagtatgtt aactccattt gcagcttcat
    29101 ttaaaagaaa taatccaaat aaaaaattag tatatataat gactgatgga gcatctcttc
    29161 caatttattt aagtaaaaat gtggacactt taaaagaaaa aaaattaata gatagtacga
    29221 taactatagg aaatgcgttt ggtggagatt atgaatgtat aaatatatat actgcattaa
    29281 ttacagcaaa agaaatacta aaagcggatg cagtatttgt cagtatggga cctggaattg
    29341 ctgggacagg aacaaaatat ggatttacag ggattgaaca aggctctatt cttgatgccg
    29401 taaaaaaact tgaaggaaga gcaattgcaa ttccaagaat tagttttgct gacaaacgag
    29461 aaagacatca aggtatttca catcatagta tgactgtatt taaagaaatt gtaaatgtaa
    29521 atgtagacat accaataact atatatgatg atgaaaaatt gaactttata aaagagcaaa
    29581 tcaatttaaa tggtttagac aaaaagcata atataattta tatagataat aataaaacga
    29641 aagaggattt agactatttt aatctaaaag taaagagtat gggaagaaat tatgaacaag
    29701 atgaggcatt tttcaaagct gcaagcacag cagcatatta cttaatggag gtttaagatg
    29761 gtattagaag aaaaaactat tagtagtgat agggtttata caggtaaagt aattacttta
    29821 aaggttgata ctgttgaaat tccagggcaa ggatatcaaa aaagggagtt agtagaagtt
    29881 ggtggagcag taggaatagt tgcaatcaca gatgataata aagttgtact agttaagcaa
    29941 tttagaaaac ctatagaaaa gccaatcttt gaaattccag ctggtaaact agaaaaaaat
    30001 gaaagtccaa aagaatgtgc tgaaagagaa ttaaaagaag aaactgggta tagtgcaaaa
    30061 aatattaagc taatacataa atttttcact tcagcaggtt tttctaatga gataatgttt
    30121 gtatatttag caacaggtct tactccagga gagaataatt tagatgcaga tgagttttta
    30181 gatgtctatg aaattgagct tgaagaagca tataacatgg tattaaaaaa tgacgttgaa
    30241 gatgcaaaaa catctattgg attattatta gtaaaagata tgtttaaaaa ttaatattta
    30301 taaaatgaat aaccctttgt cctaaagcat attattttat aaaggacaag ggggaattaa
    30361 attgagaaga acatatagaa aaaatgattt tagagaatta aatagacaaa taattattat
    30421 aggattgttg tttatgatgt ctattgttgt tgggtcttac ataaataaaa tattgccagg
    30481 ttctagtaac aatatattaa acaatataaa tcctgctgta gagtattata atctaaatat
    30541 aagcataaaa gatacagtga tacaaaattt aaaatcagat gcaatcttta tgggctcaat
    30601 agctctatta agtctatttg tagtaacaat tccagcagtg ttggtagctt ttgtattaaa
    30661 agggatgtca attggttata caataaatag ttgtatattg gcattaaaat ttaaaagtat
    30721 aaaaatgatt ttaataatat tatttaaaaa tttgattatt attccatgtg caatcatatt
    30781 agcattaatt agtttaagtt attttaaaga aatggtttat gaatttaaaa agaaaaatag
    30841 aaaaaatatg caatttttaa ttaagaggta tatactaaat attataatta taatagcact
    30901 atcattgggg ttgcaattaa tattaaatac tgtaagtatt ggtattatta agttcctagc
    30961 taaataaagg agtttataat ggatattata gagggatata tagattacat aaaagataaa
    31021 aaaaaattat cagagaacac agtggcatca tactttatgg atataaaaaa atatatggaa
    31081 tatataaatc aaaaaagcat taaactagta gatatagtag aaaatgatat tataagttac
    31141 ttaataaagt tggaaaaaga taatgtatca atagctacaa tagcgaggat gatatcctct
    31201 ataaaatctt ttcatgatta tttattttta aatcatatat gtacaaataa tcctgcaaag
    31261 gatataaaaa aacctaaaat aaaaaaagaa aatataaata tacttacaga agaagaaatt
    31321 gaaagactat taaattttcc aaagttaaca acacctaaat tgattaggga caaggctatt
    31381 tttgaagtat tatatggtac tggtataaaa gtatctgaat tagtagagat gaatatagag
    31441 gatattgacc tagatattga ttatatatat tgtaattcat gttgtaaaaa tcaaagagtg
    31501 ataccacttt gtgatattac aaaactttac ttagaaaagt atctaaaaga agcaagacct
    31561 aaaatggctg tagaaggtga aaaatcatta ttcgtaagtt cattaggtca aaggtttaca
    31621 cgacaaggtc tttggaaagt cataaaaaaa tattctaatc tagccaatat agataaaaat
    31681 ataaatccta caatgcttag acattcattt gctattcatt tgttaaatga aggtgctaac
    31741 attgctgttg taagtaaaat tttaggaaat gttaatttat ctagtttgca agtatattta
    31801 aatcatatag ataaaaacgt gagaagagaa ataaaggaaa aacatcctag aaatgatgtt
    31861 gatgtagaat tacaaaaagc agaagctaaa tagactatat agcaaatttt ataatttaaa
    31921 aggagtatat ttatatgagc agagtaattt ggattgtaat tgatagtgtt gggataggag
    31981 cattacctga tgctgaaaaa tttggtgata gtaaggatgt aagtaccctt ggaaatatat
    32041 ttaaggaata tccagatatt cagattccta atatgagaaa tttaggtata ggaaacatag
    32101 atggtataga tttttttgaa agtataaaag aaccaatagg ttgttttgga aagtgtaagg
    32161 aaatgtctca gggaaaagat actactacag gtcattggga aatgactggt ataatagttg
    32221 acaaaccatt taagacattt gaacatggat tctctaagga gataatagaa gagtttgaga
    32281 aaaaaactgg aagaaaagta gtaggaaata aaccagcttc aggaactgta ataatagatg
    32341 agtatggaga gcatcaaata aaaactggag atgtaatagt atatacatca gctgatagtg
    32401 tatttcagat tgctgctaat gaagaagtaa ttcccctaga agaactctat aatatgtgca
    32461 aaatcgctag ggaaataatg atgggggata atgcagtagc tagagtaatt gctagaccat
    32521 tcataggtaa gaaaaaaggt gagtttgtga ggacttcaaa tagaagagat tattcactag
    32581 acccatttga accaacagtt cttgataata taaaagaatc aggacttgat gttttagcag
    32641 ttggaaaaat agaagatatt tttaatggaa aaggaattac agatgctatc cacactaaaa
    32701 gcaatatgga tggagtggat gaaactttaa attatatgaa acaagataat aaaggtctaa
    32761 tatattcaaa tctcgttgat tttgattcta aatatggtca tagaagagac ccagaaggat
    32821 ataagaaagc tcttgaagag tttgatagta gacttcctga aataatggct aatatgagag
    32881 aagatgatat tttaataatc aatgcagacc atggaaatga cccaacatat aaaggtacag
    32941 accatacaag agaatatata cctgttatga tttatggcaa taagattaaa aaaggtttta
    33001 atttaggagt aaaagatact tttgctgata ttggagcaac tgttgcagat atactaaatg
    33061 taaagttgcc taaacatggg tcaagcttta aagaagattt attttaaact ataaaatgat
    33121 aaaaacttag cttaatagga tggagagatt ggaatgtttg aaaagatagc acaaagtagt
    33181 aaatttataa attctaaaag taatataaaa cctaagattg gtttgatatt aggttctgga
    33241 ctaggtgatt tagcaaatga tatagaagac cctgtcacaa ttaaatatag tgaaattcca
    33301 aacttccctg tatcaacagt agcaggacat gctggacaac ttgtaattgg aaaacttgaa
    33361 ggaaaagaag ttattgctat gcaaggtaga tttcattact atgaaggata ttctcagaaa
    33421 gaagctactt ttccagtaag ggttatgaaa gaattaggag tcgagattct tatagtaact
    33481 aacgctgctg gaggcgtaaa taaagaattt aaagcaggag atttgatgct tataagagac
    33541 catataaact ttagtggaag taatccatta gtaggaaaaa atgatgacag atttggtgct
    33601 agatttccag atatgtcaga tgcatattct tctaaatatg tagatgtagt taaaaactgc
    33661 gctaaagaat gtaatataga tgtcaaagaa ggcgtttata tgttttttag tggtccaaac
    33721 tatgaaacac cagcagaagt gagaatggca caaattttgg gagctgatgc tgtaggtatg
    33781 tctactgttc cagaagtcat agtagcttct cactccaata taggagttat tggtatatct
    33841 tgtataacaa atatggctgc tggcatctta aatcaaccac taagtcatga ggaagtaata
    33901 gaaactactc aaaaagttaa atcagaattt atgaatttag ttaaaacaac ggttaaaaat
    33961 ttataagtta aagcttatta tatatttttt gaggaagttt ataataaaat agttaatgtt
    34021 taatttgaaa agaattaata aaaagctgtc ttgatataga aagttatttc taataaagga
    34081 cagttttttt ataatactac aacatttgaa agtgttttaa agtttaatat ttatttaaat
    34141 tattatagat tagaatggag atttcaatta tgagaatgta tgatattata aaaaaaaaga
    34201 gagataactt agagcttagt aaaaaagaga taaactactt tatagaaaac tattcaaagg
    34261 gagatatacc tgactatcaa gcttctgcac tattaatgtc gatttattta aataagatga
    34321 ataaacagga aactgtacat cttacagaag caatgatgaa ctctggagac atgataaacc
    34381 tgtctgaggt agatggaata aaggtagata agcatagtac tggtggtgtt ggagataaga
    34441 ctaccatagc tcttatacct ttggttgctt catgtggagc accagttgca aagatgtcag
    34501 gtagaggtct tggtcatact ggtggtacta ttgataaatt ggagtctata ccaggttttt
    34561 cgacagaaat ggagataagt aaatttattg attctgtaaa taagtctaaa atagcagttt
    34621 gtggtcaaag tgcaaaggta gcaattgcag ataagaaaat atatgctctt agagatgtta
    34681 cagcaactgt tgataatata tcattgatat catcaagtgt aatgtctaaa aagttagctt
    34741 caggagctga tgcaattgtg ttagatgtaa aaactggtga tggagcattt atgaagacat
    34801 tagaagagtc ttttgagtta gctaaatcta tggttgatat aggtactggt atgaatagag
    34861 atacaatagg gattgtaaca gatatggatg aacctttagg ttttgcagtg ggaaactctt
    34921 tagaagttat agaagcaatt gagacattaa aaggaaatgg ccctaaagac cttgttgagc
    34981 tatgtgaagt attaggagca tatatgttgg ttttatcaaa ggtagcacat gattttgaag
    35041 atggtgttga aaagataaga gaatcaataa aatctggtac tgctatacaa aaactcaaag
    35101 agtttataga gaatcaaggt ggtaataaag atgtagtaga tgattattct ttattcaaaa
    35161 aatcaaagta taaatatcca gttaaatcaa ttaaatcagg ttatataagt aaaattaaag
    35221 ctgaagatgt tggtgtatca gctatgattc ttggagcagg tagagaaact aaagatgata
    35281 ttttagattt atcagcaggg ataattttag aaaagaaagt aggagattat gtaaatgaag
    35341 gtgaagtttt agcttacatg tattataatg atgaacaaaa gctttcattg gcaaagggaa
    35401 gatttattga ttcctactct attgtagatt caaaaataga aaaaaataaa ttaatatatg
    35461 gtattgtaac taaagaagag attaaaaagt tttagaaaat tttagaaaag tgattataaa
    35521 aggcctaaca tgtcaaaaat aatgttaggt tttttatttt ttgataaact ataaattat
//
LOCUS       DAMJWP010000013        34760 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.014, whole genome shotgun sequence.
ACCESSION   DAMJWP010000013 DAMJWP010000000
VERSION     DAMJWP010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34760
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.014"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(122..382)
                     /locus_tag="PC700_000609"
     CDS             complement(122..382)
                     /locus_tag="PC700_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2579933.1"
                     /translation="MDKLITELSSLGAIGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVQMGIMKTDVEEIKEDVTDIKGMLQNGV"
     gene            complement(645..998)
                     /locus_tag="PC700_000610"
     CDS             complement(645..998)
                     /locus_tag="PC700_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2579934.1"
                     /translation="MFKFDKNKIEQIKQGRKVEMQYKDISDISIGQAKQDDDITNNFI
                     ANAEIYEMLLSQSSVNEASNISTFSVRKSGGESGMVEVYVALILRGRKTIEEVPAVIR
                     EQVRIRCKELEIPVE"
     gene            complement(1015..6192)
                     /locus_tag="PC700_000611"
     CDS             complement(1015..6192)
                     /locus_tag="PC700_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome condensation regulator"
                     /protein_id="HDF2579935.1"
                     /translation="MKQNKLLQRGAYFNDKNILIDDFDKRYNDYDFVEFFTGISNSTF
                     GLKSDGNLYACGDNTGFQLGLGKDSSERRMFSKVKIDNVKYVSCGSKHSVAVTKDGFA
                     YGAGTSNVGQLGVIESTVYYEFTKLPIDDVKTVACGYDFTFVLKNDGTLYSAGLNSSG
                     QLGLGDTNNRATFTKVNIDSVKDVVTYNQSVFIIKMDGTAHACGLNSNGQLGINSTLN
                     KSVFNKIEGMDNVKQIACGSSHTILIKNDGTMYTTGYNGVGQLGTGNNNNSIVFTLSS
                     INNVKYASCGNNHTMILKYDNTLFSTGQNNYGQLANANKDVASRNTFAKVNVENIKDI
                     KCGSQFNFLINGSKEIFVSGCNLAGQLGSFFHTTFLYEFSNVQSSNLDNYSGLLVNDD
                     YLYVTKDNSEFLNVKLSDNFQDYKKIELTDSNMFIVMNDGTLYACGLNNYGQLGLGDT
                     VNRSVMTKVDIDNVLDIKGNGNSTFVLKNNGTLYSCGLNSNGQLGLRDEVNRNIFTKI
                     EIENVKDFCVGSNYVIALNHSKEVYGWGNNPYNNIEKTSNYPYKQGISNIEKIAAYDY
                     SVYMINSEGKLYVSGYNYNYQLGKGNNSNQSKALVSQCRTNSTSSTSNGLRTLPKITN
                     VFPFYDGCAIIDEGGYVYLTGYHGYLRTLNSSPSISDYSRYGTFIEATNSNHNTYFIQ
                     ETDFSGIEKVIGMSNNILFFKKGSSYITGYPKTFGSTITGHRSYTSINSESSNLGSNF
                     IIYHSNSKLYGKGIANSGQFGNSTNIDGTSNYDTGLKDIKDIIVKGNTVVVVDKNNNI
                     YVTGMNQNNKLGIGEYNNEPVKKFTNITEQSNSFIFMDDIKEITTSRNTMFIVKNDGT
                     AYATGNNSSGQLGLGDTINRNKFTQINLDNIKKISTSIDGNTTFAIRNDGTLYSTGLN
                     TKGQLGLGDIVNRNTFTKVNIQNVRDVVLGTTHSHAIKDDNTLYSCGENTHGQLGLGS
                     ESNHPDVLTFTVNNITNVRDVYCSDTTTFIVKDTNIAYCCGYNNNSQLGMGNTTDQYS
                     FIKCMENVKEVIPNEINTYIITIYNTAYSTGLNTDYCLGLNSNSNQSSFSEIPISNVV
                     KVAPNRNNAVLLLTSEGDVYTAGKCSNGSGTGSETPEKIKKIASKAKDIGMNYRCGHY
                     VSDNGDLYGTGFNNNGQLGVGDVTKRDTFIKTNTRVKKILPLEYANIAIKDTNDIYIC
                     GLNNYGQLGVGNRYDSRNNDNRIFNYKHMNFVMGDLTSIKNRHNFILLNNKIVIPTTK
                     DIDYGLVLGNLYKGDLYTELPYEDIKEVSISKTHIIILLNDGTMYGCGTNYHGELLQD
                     LSINQVDEFVQINVSDVKHVSCGDNFTYFIKSDDSLWSIGKNSEYQLGIGHNNPVTEL
                     QRITTISSCKEVHCGKNYTLVVTTGNELFVQGYNDKGALGLGSDSENTIIKFFTKALT
                     DIREIKSYGSDHILVLKNDNSVWVTGKNRDVYKIEQPVEFLKEFTIVPISEDVNTVKD
                     VLATDNTLYIISEVGTTNAAIEITEKSISSIKIKIQDPNKDISRIEMLINGESVKSVS
                     DLTTEKISFEVPPDKIKIGENKILFRAYCKGDDLYASLFIFKESTGNSIIKDSYVMIG
                     NRMYKVVNTTSNEQDITITLDRGLEEDLNLGDPIYQLINKTKVQVKINKSDLFKDMKL
                     VEIKKSDSSYQEIYELEEANIKSAQPKIIVEKGDKWTAIKRPSMIFRYDAENNEPQA"
     gene            complement(6214..7200)
                     /locus_tag="PC700_000612"
     CDS             complement(6214..7200)
                     /locus_tag="PC700_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDF2579936.1"
                     /translation="MEEKFYIILTKIGREKIANATALGELVGLTKFQVGDSNGEYYEP
                     TEEQTALKNVVWEGNINSLRIDEKNPNWIVIETILPGTVGGFMIREAAVLDNENNIIA
                     IGKYPETYKPRAEDGSIKDLVVKMILQLSNTSNVTLEVDPTLVFVTQKDIQDLDDKFD
                     KNIKEIKVKIGDTDILTTDSKDLSGAINEVVKKIENISFDDVISGQIQTDISVLKNSY
                     NKLSEKVLDILIYLELESEVTVDEAGYWYDTLANGNNIVAIEGLKLDLNRKCITGEIG
                     NVIFRDVVLPFSANRVRYIHDMDNNFVETKSSNTYLKEQKDITLSKYSYEIR"
     gene            complement(7201..7899)
                     /locus_tag="PC700_000613"
     CDS             complement(7201..7899)
                     /locus_tag="PC700_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HDF2579937.1"
                     /translation="MKLIDKLPSFDRNYIVEEIQGAYDTELNILKEDIDDTFNQLFVD
                     TATWGLDMWEDILCIEKKELDFDTRRSNIKAKMRSRGTSTIEVIKSICEAYTKSETDI
                     KVYSDEFTFVLSFIANNCDYKTLLDCSEMIERVKPAHLLHYLEPIILDKSMVYCGGGM
                     VCSEEVKVHPYFEPIIKCSAVVNCGAGMLSREEIKVYPLSIKCIENNCKINIAIANDT
                     GVENVVVYPKSEVV"
     gene            complement(7896..8948)
                     /locus_tag="PC700_000614"
     CDS             complement(7896..8948)
                     /locus_tag="PC700_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDF2579938.1"
                     /translation="MYSDQTYEVIKNRTLENINLDIYKGEGSFLNNMVSGNNLELSKI
                     YLELSKIHKMAFIQDTYNQFLDKRVNEFGVYRKLGTESNGEVEFIGEKGTVINNGTII
                     SYRDLLFVVIKDVTIGSEEGDNSPVQALEVGKKYNLPTNCEFKLVDNISGVTKITNTR
                     SFEGGTDIETDEELKERFYKIQRNQATSGNKAHYEEWALEVDGVYNVKVYPRWDGPGT
                     VKVLIFGENNQAVDTETIERCQQHIDEEKPIGPTITVVTPLPIEISISAVMKLEDGYT
                     LDNVKESFLESINTYFRDIRGEIIYTKVMGILINTTGVHDLSNLLINGSTDNITINED
                     KIPSVTTVNFSEVENQ"
     gene            complement(8941..9369)
                     /locus_tag="PC700_000615"
     CDS             complement(8941..9369)
                     /locus_tag="PC700_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HDF2579939.1"
                     /translation="MSTIFPFIGVPEDYILPKTEELPIFREVAWDFEKDEPILEKGDF
                     KIIEKKEALKVWIYKCIKTNRYEHEIYSLEYGTELSELIGQKYTKGLTESEASRFIKE
                     ALLINPYILEVNVKSANFNRDVLSANVKVSTIYGEVEINV"
     gene            complement(9369..9695)
                     /locus_tag="PC700_000616"
     CDS             complement(9369..9695)
                     /locus_tag="PC700_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HDF2579940.1"
                     /translation="MANPINEFIGIIREEGKYHNQPSFFIGKIKSKLPDLKIETNNII
                     LEKEDILIDSWMIDRQLETFDTETNQEHQHEVKNPFIDNFESGDMVIMFRIGEKFAVV
                     SKLVSL"
     gene            complement(9711..11240)
                     /locus_tag="PC700_000617"
     CDS             complement(9711..11240)
                     /locus_tag="PC700_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF2579941.1"
                     /translation="MIINRSKDSSSNEISFVSKDMGFLLTQSEVSYNFKDKLVEDIAK
                     QVFAENRLSVGTIAKTNVKYTKMFIGVNGYDTIMSAYTEASKKTKKKYMIEANLDKFN
                     VIEKGTVTLSVMFEEGFNIINTTFSESMENVKNKVIVVDQYGSKISEKIDNEIFKEVN
                     VIMQKVIQQQENQDVDIDSEFNGIEKSCSLKGYGDVSCITGRGVKVKDSYTKLVGLFY
                     IDTDKHTWQNGEYQIELELNFQNLMDEKSAGQDEPKEESNLGGEDYAGGKEFTAEFTA
                     YCPRKEEGGDTDCRKKKLDPSKKTCAAPMVGKYEQTYYTKEFLNKHPLLNYGDEIQVI
                     TGVSGRDGVYKVNDVGPAITIEKNGTYHIDILFGNVEEASKFGRRKGKIIIGGYSGNV
                     SDKAKIVISEAKKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVSLPRTSNQQSKKGK
                     KVEQKNLQAGDLVFFHNPVSHVGLYIGNGEFLHAPQKGDVVKISKLSSRRDFNTARRV
                     L"
     gene            complement(11522..11944)
                     /locus_tag="PC700_000618"
     CDS             complement(11522..11944)
                     /locus_tag="PC700_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2579942.1"
                     /translation="MEMWLRQAEDRFRFPVFPSSFSINGKAAVNSSSILKIGEVATFG
                     GVALKSISISSFFPNKDYTFCDYTGFPSPYDCVNKIEKWMKEGFILRFTITETNINME
                     VIIEGFSYEERDGTRDVYFTLDLKEYKRIKIPKVTPKQ"
     gene            complement(11944..14397)
                     /locus_tag="PC700_000619"
     CDS             complement(11944..14397)
                     /locus_tag="PC700_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2579943.1"
                     /translation="MGNVREEGINMYLTDNYTPKMNQIISVTDNFRRATVAVSLSTNV
                     MASSIKNSIGSASSRVNSLNSSLRKVQTTASSVSSTMAKLSSSINAVSGVIGSLNGSI
                     MRLAITIAMIIDYFNKLIQKKNEFNSNIMIILIFKAKSDEVEKTKNKLLGNLKKIGGK
                     IWNIVIKAKDMTKRVISSILGKLKRVEKRPYQGSINLKDMVSSAMARILPKLMLFKNT
                     FWSGVIAIKDMASSIISKVFPKLRLFAGKVWSGAIAVKDMASGILGSIKGKISDLTNG
                     ATIGVAVKKGVDLLGQEQNQKVVLESVMKRNTGKTSQKDVDKYYDSLVNMANDTPFDP
                     EDVVAMGTKAKMISNITGGKKEKDITQAMVDVRALNMNTSSEQDVSAAFLSAAKGNME
                     SLNTLVGENYKTFDEALEGISVKQMGLAKEMSNTIPGIISGAQTSINNGLKSIVKPFD
                     DILGQGLKKIKTFIESGLGNLAGLSEKMAGKIGNVMNGKIIIGNKYDQMQSRSVKNGK
                     EFSDSTQYRISNEAEKRKMMVENKQERFENHAATMIGNAPKAIVNAGSTLLQNIDFTA
                     LIDSLLPVVNLVNNLLDSINNKSPIAQGLISIFGTIVTTAFQLIGPVVEAVSPIITRI
                     FTFLGEYAPQINNFIETLGVIWKTVWETLGPLLETGWKIIEPILGAFFNILDKVCKIV
                     KDICKWWQTMINKIKNGSITGTVLNLVEKSKKNYKDNPYAGTKAGDSGKAYSSKKGNN
                     AFGLNYVPYNDYQTRLHEGEMVLTKQEANQYRSRKNGGNINIAKLADTIVIREEADIE
                     KITSKLVASIQLAQLGGVL"
     gene            complement(14397..14564)
                     /locus_tag="PC700_000620"
     CDS             complement(14397..14564)
                     /locus_tag="PC700_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2579944.1"
                     /translation="MYYYWKKKGIRPSLFYAMDKGELKLIEAFFALEIEEEVEKMKHG
                     YGVCPLTGGGM"
     gene            complement(14591..15037)
                     /locus_tag="PC700_000621"
     CDS             complement(14591..15037)
                     /locus_tag="PC700_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2579945.1"
                     /translation="MNENGLSKNINIVDLLLNADTENLERPSTIVELKRLSTIFGQEF
                     KVMCRALTISKDEEIQNTCLKIDENMKTDIDLPEMQMLTIIEGVCDLDGKLLFKNKEL
                     MDKFKAPTPKELARKLLLPGEITNLYRILQDVMGYGKNAVIEEVKN"
     gene            complement(15151..15579)
                     /locus_tag="PC700_000622"
     CDS             complement(15151..15579)
                     /locus_tag="PC700_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HDF2579946.1"
                     /translation="MANMEARNVMSGTWGELWLDGNKVAEVKKFQAKMEFTKEDIIIA
                     GQMGTDTKYMGYKGKGSITLYHVSSRMHKLIGEKIKRGSEPRFVAISKLNDPDSYGAE
                     RIAVKNIAFDDLTLADWEVGVKGEIEAPFTFTEYDFLDII"
     gene            complement(15593..16657)
                     /locus_tag="PC700_000623"
     CDS             complement(15593..16657)
                     /locus_tag="PC700_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HDF2579947.1"
                     /translation="MAIGLPSINISFKELATTVKERSARGIIAMVLKDAKALGLNEIH
                     EKEDIPVDLSAENKEYINLALMGNVNTPNKLLVYVIEGEADIQTALDFLETKEFNYLC
                     MPKAVEADKTAIKNWIIKLRDIDKVKVKAVLGKVVGNHEGIINFTTEDVLVGEKKYSV
                     DEFTSRVAGLIAGTPLSQSVTYTKLSDVVDIPKMTKVDAESRVNKGELILIKEAGAIR
                     IARGVNSLTELTEEKGEMFQKIKIVDTLDIIHSDIRKVIIDDYIGKVTNSYDNKCLLI
                     VAIKSYLEELEKSALIESDSTVEIDFEAQKSYLKSKGVDLSYMTLQEIKEANTGSKVF
                     LKAKIKVLDAMEDIDLSIEI"
     gene            complement(16662..17105)
                     /locus_tag="PC700_000624"
     CDS             complement(16662..17105)
                     /locus_tag="PC700_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2579948.1"
                     /translation="MLKYKEILETIIEILKKNFTESIFIDDESVQGSEGSCFFVSILS
                     VICTPIMLNTNNKDIVISIKYLPKPQSKSIRMYEISDELNKLFNRNIKVTDRKLNITK
                     LEQSIKKEESIYVLNFTITLNYLDSVYEEDVVYENMEEINLNLGE"
     gene            complement(17354..17794)
                     /locus_tag="PC700_000625"
     CDS             complement(17354..17794)
                     /locus_tag="PC700_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2579949.1"
                     /translation="MENKKDILFKETDERLHNYKYLDIKIKNINLDIKRCENEYSGCG
                     AMVYTEKTSNTYNISSSVENEVLKREERLRKLKMEKEDIEIEKEKIENALTCLNDIEM
                     EFFNLFYNSKTKNNMTYISMKLHLDRTSCYNLKKKMIFKLSEIL"
     gene            complement(17899..18096)
                     /locus_tag="PC700_000626"
     CDS             complement(17899..18096)
                     /locus_tag="PC700_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2579950.1"
                     /translation="MFKNNLKYYRKCKGMTQIQLARKAGITNDYISQIERGIKNPGLL
                     MAKKISSILEQNIEEVFFIQL"
     gene            18265..18585
                     /locus_tag="PC700_000627"
     CDS             18265..18585
                     /locus_tag="PC700_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDF2579951.1"
                     /translation="MFAKRLRELRKEFGLTQRELGEKVGVSQRVLGYYETENRFPDEH
                     ILNKLADVFNVSVDYLLGRTLVKENIDTVAAHRKNPHEELPEEAQEQLNDYIEFLLNK
                     YKKK"
     gene            18667..19173
                     /locus_tag="PC700_000628"
     CDS             18667..19173
                     /locus_tag="PC700_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2579952.1"
                     /translation="MNNLDKLFELASQEEIIIHYTTYIAGDLEGLYINKHGIKIISLL
                     SNLKQNSKKLTSILAEELGHHFTSLGYYVSSYNDYYTKIIIDKCENKALKWACEFLIT
                     EEDIINIINSGITCVYEMADILNVDITFFQKRLEFLSLKKQSLQLGNNKYLILTNLPY
                     FYIFDPIS"
     gene            19874..20629
                     /locus_tag="PC700_000629"
     CDS             19874..20629
                     /locus_tag="PC700_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2579953.1"
                     /translation="MEKKVLATVGEKEITNIDIENALKSLDPYQAMQFKTEEGKKHLL
                     NDLVNQELFFLDAKEEKLDEEEIFKLEMKKIEENVLKQFAINKVLSSVNVTEDEKVKF
                     FEANKSSFSKPESATAKHILVDSDEKAKEILAQIKSEEISFEDAALKHSSCPSKDMGG
                     DLGTFGRGQMVPEFEEAVFSMAKGEVSEPVKTQFGYHIIKLEDLQESTESTFDEVKAE
                     VEKSLLYQKQNEVYGNKINALNAKYGNLVKYND"
     gene            complement(20772..21200)
                     /locus_tag="PC700_000630"
     CDS             complement(20772..21200)
                     /locus_tag="PC700_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDF2579954.1"
                     /translation="MNILFFLTPKSEVAYIYEDYTIRQALEKMEYHKYSAIPIISKDG
                     KYVGTITEGDFLWTLKNDLNLDLKGLEDVPVTDINRKMDNSPVSINADIEDLVIKSLN
                     QNFIPVIDDQDTFIGIIKRRDVIGYCYEIIRGYKNLANDN"
     gene            complement(21621..21821)
                     /locus_tag="PC700_000631"
     CDS             complement(21621..21821)
                     /locus_tag="PC700_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDF2579955.1"
                     /translation="MKTGVVKWFNNEKGFGFISVEGEGDVFVHFSAITGDGYKTLEEG
                     QSVEFEVVDGAKGPQAANVVRL"
     gene            complement(22004..23173)
                     /locus_tag="PC700_000632"
     CDS             complement(22004..23173)
                     /locus_tag="PC700_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HDF2579956.1"
                     /translation="MGGVALKILKNKNKYIKGIMAFVFLSLATTAGVFLINDLSMKNM
                     EEKVMESANVEGDDVKDQYKLLLENLFDYRNKAILEKNENILKGLYETDKKFGLWAYE
                     NEVKKMKYLENWSSKQGVKFKDIKTRVKIKKIKEKEKDLYGIICSVSTEYKYSYENQK
                     DKENAFRIGTYHYLNVKIIDNQYVITKEWYTDPFADSLNLENIKSEDIRNYIGQQDGV
                     ELQLTDEQKKAIDYAHKYCGAAADEEHGMKFNPNYRDYNPEGGDCANFASQIMFESGR
                     FKKNSIWNYSERAGTKAWVNAQAFKNYALYSGRGSLIAKGSYEEVYKEAYNLRPGDFV
                     GYEKKGRITHVSTVTGLDSRGYPLVTCHNTDRMLVPWDLGWSDKSIRFHIIKINY"
     gene            23409..24230
                     /locus_tag="PC700_000633"
     CDS             23409..24230
                     /locus_tag="PC700_000633"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HDF2579957.1"
                     /translation="MQKKVAAINDLSGIGKCSLSVAIPILSALKVQCCPFPTAILSSQ
                     TGYPEFTFLDFTDEMVKYSNVWKNLKVNFDSIYSGFLGSKHQIEIVANFINDYPNAFI
                     VVDPVMGDNGVMYPIFTEEMRQEIKELVKHSDLTTPNLTEACFLTGNDYTKSDYNRDE
                     LIYIAKSVSDLGPSKVVITGILEDDNILNLAYDRDNDHVFFTSVKYNNCSYSGTGDIF
                     TSILCGMLVNKHDLGVAVNTATDFIYKTINYTSQFDTDRNDGVMFENFLSDLTNI"
     gene            complement(24306..25694)
                     /locus_tag="PC700_000634"
     CDS             complement(24306..25694)
                     /locus_tag="PC700_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase"
                     /protein_id="HDF2579958.1"
                     /translation="MLRSKKSNNKKVEKLRTIFIKYLSLFFVMTISFALLLMLSFSVL
                     LSSGVILPANYSEKQFDKYKEKIISSEKAIEDIIPNLYEYGVYTLDGNLISGTFNKKE
                     SKQVWNLMRDIEERHAYSESYIKFFKKDEVFIIKYKLVSEYSSPVLRAYLPKPEMLGM
                     IIFSIIFFIEIVILSKVFGKKFNIEMELLKNTTEKIEQQDLDFVVESSKIYEINNILF
                     SMDKMKLALKNSLEKQWMLEENRKEQISALAHDIKTPLTIIHGNTDLLIEINENPELL
                     EYMEYISKGVTQIEKYINTLIDISKTENGYILNKEAINMSEFIKDVLLQIEALASTKN
                     LDVEFYKQDNLPKNITIDRELLFRAIMNIISNAIDYSPSQSKLYISVSVSNKYLKFVV
                     TDCGSGFSKADLSKATEQFYTGDLSRNSKSHYGMGLYIVNNIVQKHNGILYIENSVKT
                     GGAMVTIEIPIV"
     gene            complement(25838..26596)
                     /locus_tag="PC700_000635"
     CDS             complement(25838..26596)
                     /locus_tag="PC700_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HDF2579959.1"
                     /translation="MRQLIRIFCSDLIKLKRNFIILMHICIALMGMGLFLWYYKVSGA
                     DNILKIVAYLQVIAIAFPLLSGVVCSMFVEQEYYAGNYKHMLTTSNPKYLTLISKYII
                     LVCLGFVATLVSVLGFKFGISKAYFTSSFYMISILILIGCNLFEYILHLFLSLRFGKG
                     TSIGVGIVEMLLSALLLTGLGSGIWQYFPCAWGVRFITIWSSFSSSKTVEYIKVESIK
                     AYQSTGLMCGFVTILAFVILCIWFSKWEGKKSEE"
     gene            complement(26598..27314)
                     /locus_tag="PC700_000636"
     CDS             complement(26598..27314)
                     /locus_tag="PC700_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HDF2579960.1"
                     /translation="MERFIRVEFQKMKGTFGMKLIWIVPVFCILLCGLGGGQNAAFNW
                     WYTTFLPGALTLISSLIVLKDEKSKYRGILPLPIEMKDVWAGKMIYCIICCSIASIIS
                     AIGVVFVGIIYKETISIGQNIIATILIILLFMWQIPLCMFLASKFGIFVTVLTNMFFT
                     IVGVAVFSTTEMWYVFPYSIPSRLMVVVLHILPNGLPVPEGSELLNNAVILPGVLISV
                     ILFVLFALVTRAWFNNREVK"
     gene            complement(27316..28023)
                     /locus_tag="PC700_000637"
     CDS             complement(27316..28023)
                     /locus_tag="PC700_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HDF2579961.1"
                     /translation="MNNYILQVKGVSKKFKEQMAVDNISLAIKSNSIYGLLGPNGAGK
                     STLLKMITGILNPTSGKIMFEGHRWTRKDLSNIGSLIESPAIYENLTARENLKVHTTL
                     LGLPDSRIEEVLETVDLRDTFKKRSGQFSLGMKQRLGIAIALLNHPKLLILDEPTNGL
                     DPIGIEELRKLIRSFPKQGITVILSSHILSEVEQVVDEIGIISNGVIGYQGEVSKEQN
                     LEKLFMKVVAENRKRGE"
     gene            complement(28140..28676)
                     /locus_tag="PC700_000638"
     CDS             complement(28140..28676)
                     /locus_tag="PC700_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2579962.1"
                     /translation="MNKIAVSFLIIATTLLSTACMDYSISAVELVDSKESAVVKKDED
                     AKEETTSKMINSKKTTKIPIEIISKDEKIVKYLQIDEESSLKDKLRLILDTLSNEYFN
                     GLDMEVEVKEKDNLVKINLIEPDKKSRVSWKDDYLNEQNIIYTINNIIKNVIQEEDNS
                     IWIEEVEIYYNGKLIELR"
     gene            complement(28778..29848)
                     /locus_tag="PC700_000639"
     CDS             complement(28778..29848)
                     /locus_tag="PC700_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HDF2579963.1"
                     /translation="MNKKLAIFAAVLLVIGIIGTTWSGILVMPSLINFGLEKEAEFKK
                     ENKLYQEKVNIDKLDISVDNINVTIKKSSSEDVRVTTRGNNEFYKYNVTLKDKTLVVK
                     GERKYENKIKKIKNFDQLLNTSINSMFSHDYREIIIYVPNNVDINASSTSSYLFVYDN
                     VASNTITYKTSYGGFSRAITENKVNRLENLNLISNSNLHLSTKSILGVKNVNIESESL
                     DISSENEDVFINNIEEYIPENVNIKEKIGRNSNYESEFYLYSNMPIAKFLDIEVPNSK
                     VRLDIPVNKYKFNCDIKSKEAIEEFNNDEEYDNDSYDYEHSEEHSNKYRNTREIKGLL
                     NKNLSNLEKEYTIKINSNSLEL"
     gene            complement(29841..30899)
                     /locus_tag="PC700_000640"
     CDS             complement(29841..30899)
                     /locus_tag="PC700_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HDF2579964.1"
                     /translation="MNLNKTEFLEILKDYLKKDFSESEVNDILRDYEEYFVDGLIEGK
                     SDMEIIASLGSPKSIAKELILQTKEKNIEEEKIINTDVLNDKFSKFKSDMKKGYSILK
                     DEAKEKYSKSKENIDGKIMNTDILNGKFSRFKSNIKKDYSILKDEAKEKYNKSKENIN
                     DKLTPSIEEGVGGLSRQVIKGLLVVLSLFLIVPAFAFVCAIVSVTIALVCSLIAFVIL
                     IPFVIKFIAMVPDVMMFCIFATIAFVGLEILAWQILLFAIKLGKQLLNNYIHWIKRKN
                     IYIKANEKMEKRKKATLNDEDLNKDINEDSYNNDYYNREEESIKETDEYNDDSINFEA
                     IYKDLEDIENKKGEDKHE"
     gene            complement(30886..31218)
                     /locus_tag="PC700_000641"
     CDS             complement(30886..31218)
                     /locus_tag="PC700_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDF2579965.1"
                     /translation="MNTQFRKGVLEICVLALISKKDMYGYEIVHNISKVIDVNEGTIY
                     PILRRLTKDLYFETYILESNEGPARKYYRITSLGKENLSNLMEEWREFVKAVEILISE
                     DEGGNELE"
     gene            31417..32538
                     /locus_tag="PC700_000642"
     CDS             31417..32538
                     /locus_tag="PC700_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HDF2579966.1"
                     /translation="MKDDKTILILTAQFGAGHISAAKAVKECIIEKYSNYNVVIQNFI
                     NASIPMMNKPMVKLYENNTKYTPGLYNYYYYFKKSFDSRHDLSHKLYTPKLSEYIADI
                     NPDLIISTFPLAAACVNNFKIKNPDINIPTLTVITDVVDSMEWVFENTDLYFVPSPEI
                     KNRFFQKGINPDSIKVTGVPVDKRFQIESKELCCDKYRLLLLGGGRGLFDIDEDFMHW
                     IDEFIEEHSNSIEITIVTGKNKKLYDNLTHKKPLKNIKVLGFVNDMYNLIRECDLMLT
                     KPGGATIFEAIQSQTPVLVKMPKVGQEIENAKFIIDKGLGMIYSDDLDLKNIFYKLVS
                     NEFDSIINFMKKNLEEFKTVIHPEKIADYISELIDNHYS"
     gene            complement(32666..34564)
                     /locus_tag="PC700_000643"
     CDS             complement(32666..34564)
                     /locus_tag="PC700_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HDF2579967.1"
                     /translation="MDSLLYVIEKDKHTNEELREILKSNKNIRFVSLMGVDLGGNATD
                     EKIPVELFLEDIDKFLESAIQTDGSSVELYNIATLNNAKVDLMPDKSCHWYVDYNMEY
                     IDEEVGLPVGTLKIPAFLIHDNKKVCSRGVLQKADKYFKKSMYEIFREYPHVINNIGI
                     DSVDDIEEIMLTAATELEFWVNTPEDKADLEKLYVSQSLKEQYWKRTHGIIRTCLEKS
                     LIILQKLGVNPEMAHKEVGGIQSSISIDGRTNHAMEQLEVSWKFSTPLQAADNELLVR
                     DVIEDVFTSHGLEVTFKAKPIHGVAGSGGHTHVGVSAKLKDGSIKNLFAPKDLKEDYL
                     SELGYGALMGLLYNYEVLNPIVTASNDGFNRLVPGFEAPVCIVTSLGHSYEIPSRNRS
                     VLVGLIRDMKNPKTVRFELRSPSPLSNTYLVIAGCYQTMLDGIKAAAKSGLSTKELEK
                     ELSKNVGEESFYLEKDRAYRDENDVFEHYSLEERNARFGIPPATVYENMKNLEIYASK
                     LKSLKQGDVFTDSIIESFKIGAIDKWQKKLKTRIIEAGIQKIRSIVKIHTKENMDALD
                     EVVWNSISDLKFNIMKDTLTRESLFTRVREAIENKDYQTASDLQIELKRSMEEIQQLY
                     MQYKKNIY"
BASE COUNT        11047 a         6032 c         3700 g        13981 t
ORIGIN      
        1 ttactttttt taattaatat tttaaaatat aaaatccttc tcaataaaat agggcactat
       61 ctacaatact actttatctt ttcctaataa tatataatct ggtgctactg ctactttcat
      121 gttatacacc gttttgtaac atacctttta tatcagtaac atcttcctta atttcctcta
      181 catctgtttt cattattccc atttgaacaa gtatattttt gtttatttct tgttgttgtg
      241 tagataattc tataaaattt tctacagttt ttttatacat gtctctatct tcttttttct
      301 cctgcatagt atttttaaat aatagagcac atagtatacc tattgccccc agactactca
      361 attcggttat taatttatcc attatagcct cctattgtct aaatttttta tacttatagc
      421 tttaattttt gttatattct tacatcatgc aatattatat tttcaggatt atcaatttaa
      481 tcttctgtac tttaaattgt aggtttaatt tatagattga ataaataata aatatatata
      541 caatttctaa tataacaaaa aataagtaca aggcttactt attaaagtta agaccttgta
      601 ctttacataa aaaaaataac taatacatag ttattctaaa tttactattc aactggtatt
      661 tctaattctt tacatctaat tctaacttgc tctctaatta ctgctggtac ttcttctatt
      721 gtttttctgc ctcttaaaat taaagctaca tatacttcta ccattccact ctcacctcca
      781 gattttctta cactaaaagt gcttatatta cttgcttcat tgacagaact ttgacttaac
      841 aacatctcat atatttctgc atttgctata aaattatttg ttatatcatc atcttgcttt
      901 gcttgaccta tacttatgtc tgaaatgtct ttatactgca tttctacttt tctaccttgt
      961 ttgatttgtt ctattttatt tttatcgaat ttaaacaatt tttacacctc cattttaagc
     1021 ttgtggctcg ttgttttcag catcatatct aaaaatcata gatggacgtt ttatagctgt
     1081 ccatttatct cctttttcta ctatgatttt aggctgagca ctttttatgt tggcttcttc
     1141 taattcatag atttcttgat aacttgagtc tgattttttg atttcaacta gtttcatgtc
     1201 tttgaataag tcagatttat ttatttttac ttgaacttta gttttattta ttaattgata
     1261 tatagggtct ccaagattta aatcttcttc aagtcctcta tctagtgtaa ttgtaatatc
     1321 ttgttcatta gatgttgtat taactacctt gtacattcta ttacctatca taacataaga
     1381 atctttaatt atagaatttc cagtactctc tttaaaaata aataaagatg catataaatc
     1441 atcaccttta caataagctc taaatagtat cttattctct cctattttaa ttttatctgg
     1501 tggtacttca aaggatattt tttcagtagt taaatcactt acagatttta cactttcacc
     1561 atttataagc atttctattc tacttatatc tttattaggg tcttgtattt ttatcttaat
     1621 tgatgaaatt gatttttcag taatttctat agcagcattt gtcgttccta cttctgatat
     1681 aatatataat gtattgtctg ttgcaagtac atcctttact gtatttacat cttcagaaat
     1741 aggtactata gtaaattctt ttaaaaattc tactggttgt tcaattttat atacatccct
     1801 attttttcca gtaacccata ctgaattatc atttttaagt actaatatat ggtcacttcc
     1861 ataagatttt atttctctta tgtctgttag tgcttttgta aagaacttaa ttatagtatt
     1921 ttcactatcg cttcctaatc ctaaagctcc cttatcatta tatccttgta caaataattc
     1981 attacctgta gttactacta atgtatagtt tttaccacaa tgtacttctt tacagctaga
     2041 tatagttgta attctttgta attcagtaac tggattattg tgacctatac ctaattgata
     2101 ttcggaattt ttaccaatag accaaagact atcatcagat tttataaaat aagtaaagtt
     2161 atctccacat gaaacatgct ttacatctga tacattaatc tgcacaaatt catccacttg
     2221 atttatagac aagtcttgca ataattctcc atggtagttt gtaccacatc catacattgt
     2281 tccatcatta agtaatataa taatatgagt cttagaaata gatacttctt ttatatcttc
     2341 atatggaagc tcagtataaa ggtctccttt gtataaattt cctaatacta aaccataatc
     2401 tatgtctttt gtggtaggta tcactatctt attgtttaga agtataaagt tatgtctgtt
     2461 tttaatagat gtcaaatcac ccattacaaa attcatatgc ttataattaa atattctatt
     2521 atcattattt ctactatcgt atctatttcc aacacctaat tgtccatagt tatttaatcc
     2581 acaaatatat atatcattag tatcttttat tgctatattt gcatattcta aaggaagtat
     2641 tttctttact cttgtattgg tttttataaa tgtatctctt tttgttacat caccaacacc
     2701 taattgtcca ttattattaa aacctgtacc atataggtct ccattatcac ttacataatg
     2761 tccacatcta taattcattc caatatcctt tgcctttgat gctatttttt taatcttctc
     2821 tggagtctca cttcctgtac ctgaaccatt actacatttg cctgcagtat atacatcccc
     2881 ttcacttgta agtaaaagta ctgcattatt tctgtttgga gctactttta ctacatttga
     2941 aattggaatt tcagaaaatg aactttgatt gctattacta tttagaccta agcaataatc
     3001 agtatttaaa cctgtactat atgcagtatt atagattgtt attatatagg tatttatttc
     3061 atttggtata acttctttta cattttccat acactttata aaactatact ggtcagtagt
     3121 atttcccata cctagttgtg aattattatt gtatccacaa caatatgcaa tgtttgtgtc
     3181 ctttacaata aatgttgttg tatctgagca gtacacatct cttacattag ttatattgtt
     3241 tacagtaaat gtcaatacgt ctggatggtt gctttcgctt cctaagccca gttgcccatg
     3301 agtgttttct ccacatgaat ataatgtgtt atcatctttg attgcatgcg agtgagtagt
     3361 ccctaaaaca acatctctta cattttggat gtttactttg gtaaatgtat ttctatttac
     3421 tatatcacct aatcccagtt gtcctttggt atttaatcct gtggagtata gtgttccatc
     3481 atttctaatt gcaaatgttg tgttaccatc tatacttgtt gatattttct ttatattatc
     3541 aaggtttatc tgagtgaact tatttctatt tattgtgtca cctaatccta attgtccaga
     3601 actattattt cctgtggcat aggctgttcc atcatttttt actataaaca ttgtatttct
     3661 tgatgttgta atttctttta tatcatccat aaatataaat gagtttgatt gttcagttat
     3721 atttgtgaat ttttttactg gttcgttgtt atattcccct atcccaagtt tgttattctg
     3781 attcattcct gttacatata tattgttatt tttatctact actactacag tatttccttt
     3841 tacaattata tcttttatgt cttttaatcc tgtatcatag ttacttgtgc catctatatt
     3901 tgttgaattc ccaaattgcc cactattagc aatccctttt ccatataact tggaattact
     3961 atgatatatt ataaaattac ttcctaaatt agaactctca gaattaatac tagtataact
     4021 tctatgtcca gtaatggttg agccaaatgt ttttggatat ccagtaatat atgaacttcc
     4081 tttcttaaaa aataatatat tatttgacat ccctattact ttttcaattc cactaaaatc
     4141 agtctcttgt ataaaataag tattatgatt tgaatttgta gcctcaataa aagttccata
     4201 tcttgaataa tcagatatac ttggactgct atttaatgtt cttaaatatc catgatatcc
     4261 tgttaaataa acataacctc cttcgtcaat tattgcacaa ccatcataaa aaggaaaaac
     4321 attagttatt ttaggtaacg ttctaagtcc atttgatgta gaagatgttg aatttgttct
     4381 acattgagat actaatgctt tgctttggtt actattattt cctttaccta attgataatt
     4441 ataattgtat ccagaaacat atagtttccc ttcactgttt atcatatata cagaataatc
     4501 atatgctgct atcttttcaa tattacttat tccctgctta tatggataat tagaagtttt
     4561 ttctatatta ttataaggat tatttcccca tccatatact tcttttgagt gatttaaagc
     4621 tatgacataa ttgcttccta cacaaaaatc ctttacattc tctatttcta tttttgtaaa
     4681 tatatttcta ttaacttcat ctcttaaacc caattgtcca ttactattta aaccacatga
     4741 atataatgtt ccattattct taagcacaaa agttgagttt ccgtttcctt ttatatccaa
     4801 aacattatct atatccacct tagtcataac tgacctgtta acagtatctc ccaatcctaa
     4861 ctgtccataa ttatttaaac cacaagcata caatgtacca tcattcataa caataaacat
     4921 attgctatct gttaactcta tcttcttata atcttgaaaa ttatcactta actttacatt
     4981 taaaaattca ctattgtcct ttgtaacata taaataatca tcattaacca ataaacctga
     5041 ataattatct aaatttgaag attgcacatt tgaaaactca tacagaaaag ttgtatgaaa
     5101 aaatgaacca agttgacctg ctaaattaca gccagataca aatatctctt ttgaaccatt
     5161 tattaaaaaa ttaaattgag aaccacattt aatatctttt atattttcta cattaacctt
     5221 agcaaaagta tttcttgatg ctacatcttt attggcattg gctagttgac cataattgtt
     5281 ttgtcctgta ctaaacagtg tattatcgta ttttaatatc atagtatgat tatttccaca
     5341 agaagcatac ttaacattat ttatactaga aagagtaaat acaattgaat tattattatt
     5401 tcctgtacca agctgaccaa ctccattata gcctgtagta tacatagttc catcattctt
     5461 aataagaatt gtatgactac taccacacgc tatctgtttt acattatcca taccttctat
     5521 tttattaaat acacttttat ttaaagtact attaattccc aactgcccat ttgaatttaa
     5581 tccacatgca tgtgctgtcc catccatttt tatgataaat acagattgat tataagtcac
     5641 tacatctttc acactatcta tatttacttt agtaaaagta gctctattgt tagtatcacc
     5701 tagtccaagt tgaccacttg agtttaaacc tgctgaatat aatgttccat catttttaag
     5761 cacaaatgta aagtcataac cacatgcaac agtttttaca tcatctattg gtagcttagt
     5821 aaattcataa tatactgtag actcaattac acctaattga cctacattac ttgttcctgc
     5881 tccatatgca aatccatctt tagttactgc tacactgtgt tttgaaccac aagatacata
     5941 ttttacatta tcaattttta ctttactaaa catccttctc tctgacgaat cttttccaag
     6001 tcctagttga aaacctgtat tatcgccaca agcatataaa ttaccatctg attttaaacc
     6061 aaaggtacta ttacttatac cagtaaaaaa ttctacaaaa tcataatcat tatatctttt
     6121 atcaaaatca tcaatcaata tgttcttatc attaaaataa gcaccacgct gtaaaagttt
     6181 attttgcttc attattagta ctacctcctt tatttatctt atttcatatg aatatttact
     6241 tagagttata tctttttgtt cttttaaata agtgttacta gattttgtct caacaaagtt
     6301 attatccata tcatgtatat atctaactct atttgcacta aatggtaata ctacatctct
     6361 aaaaatcaca ttaccaattt cacctgttat acattttcta tttaaatcta acttaagccc
     6421 ttctatagct actatgttat ttccatttgc taatgtatca taccaataac cagcctcatc
     6481 tacagttact tctgactcta attctaggta tattaatata tctagcactt tttcagataa
     6541 tttgttatag ctatttttta atactgatat atcagtttgt atttgaccac ttataacatc
     6601 atcaaaagat atattttcta tttttttaac tacctcattt atagctcctg ataaatcttt
     6661 agaatctgta gttaatatat ctgtatctcc aatttttact tttatttctt ttatattttt
     6721 atcaaactta tcatctaaat cttgaatatc cttttgagtt acaaaaacca acgtcgggtc
     6781 tacttctaat gtaacatttg aagtattgga caattgtaaa atcattttta caaccaaatc
     6841 tttaatactg ccatcttcag cacgtggctt atacgtctct ggatacttac ctatagctat
     6901 tatattattc tcattatcca gaacagcagc ttctcttatc ataaatccac caactgttcc
     6961 tggtaaaata gtctctataa ctatccaatt aggatttttt tcatcaattc ttagagaatt
     7021 tatatttcct tcccaaacta cattctttaa agcagtttgt tcctctgttg gctcataata
     7081 ttctccatta ctatctccaa cttgaaactt ggttaatcca acaagctctc ctagtgcagt
     7141 tgcatttgct attttttctc taccaatttt ggttaatatt atataaaatt tttcttccaa
     7201 ttataccacc tccgatttag gataaactac tacattttct acgcctgtat catttgcaat
     7261 agctatatta atcttacaat tattttcaat gcatttaatg cttaaaggat aaacctttat
     7321 ttcttctcta cttaacattc cagctccaca gtttacaaca gcactacatt ttataattgg
     7381 ttcaaagtat ggatgaactt ttacctcttc actacatacc atacctccac cacaatagac
     7441 catactttta tctagtatta ttggttctaa atagtgtaat aagtgagcag gttttactct
     7501 ttcaatcatc tcgctacaat ctaaaagagt tttatagtca cagttatttg ctataaaact
     7561 caatacgaat gtaaattcat cactataaac ttttatatct gtttctgatt ttgtatatgc
     7621 ctcacatata ctttttataa cttcaatagt actagtacct ctgcttctca ttttagcttt
     7681 tatattgcta cgtcttgtgt caaaatcaag ttcttttttt tcaatgcaga gtatgtcttc
     7741 ccacatatct aatccccatg tcgctgtatc aacaaataat tggttaaagg tatcatcaat
     7801 atcttcttta agaatattta attctgtatc gtatgcacct tgtatctcct ctacaatgta
     7861 atttctatca aatgatggta gtttatcaat tagcttcatt gattttccac ctcactaaaa
     7921 ttaacagttg ttacactagg tattttatct tcattaatag ttatattatc tgtacttcca
     7981 tttataagta gattacttaa atcgtgtaca ccagtagtat ttataagtat tcccatgact
     8041 tttgtataga ttatctctcc tctaatatct ctaaagtatg tatttatact ttctaggaaa
     8101 gattctttta cattgtctaa tgtatatcca tcttctagtt tcattactgc acttatactt
     8161 atttctattg gtaatggtgt cacaactgtt atagttggtc caataggctt ctcttcatct
     8221 atatgttgct gacacctttc aatcgtttct gtatcaacag cttgattatt ttccccaaat
     8281 atcaagacct taactgttcc tggaccatcc catcttggat aaaccttaac attatagact
     8341 ccatctactt ccaaagccca ttcttcatag tgagctttat ttccacttgt agcttgattt
     8401 ctttggattt tataaaatct ttcttttagt tcttcatctg tctctatatc tgtaccacct
     8461 tcaaaacttc ttgtgttagt aatctttgtt actccagata tattatcaac tagtttaaat
     8521 tcacaatttg taggtaaatt atatttctta ccaacttcca gagcttgaac tgggctattg
     8581 tcaccttctt cactaccaat agttacatct tttattacta caaatagtaa atctctatat
     8641 gatattattg tgccattatt tattacagtt cctttctctc caataaattc aacttctcca
     8701 tttgactctg tacctaactt tctatataca ccaaattcat tgactctttt atcaagaaac
     8761 tggttatatg tgtcttgtat aaaagccatt ttatgtatct ttgaaagttc tagatatatc
     8821 ttcgaaagtt ctagattatt tccagatacc atgttgttta gaaaagaacc ttctccttta
     8881 taaatatcaa gattaatatt ttcaagagtt ctatttttta ttacttcata tgtctggtca
     8941 ctatacattt atttccacct ccccatagat agtggatact tttacatttg cactcaatac
     9001 gtctctgtta aagttagcac tttttacgtt tacttctaat atatatggat ttattagaag
     9061 ggcctctttt atgaatctac tagcttcact ttctgtaaga ccttttgtat atttttgtcc
     9121 tattagttct gaaagctctg tcccatattc taaagagtat atctcatgtt catatctatt
     9181 tgtctttata cacttgtata tccaaacttt taaggcttct tttttttcaa ttattttaaa
     9241 gtcacctttt tctaaaatag gttcatcttt ttcaaaatcc catgccactt cacgaaagat
     9301 tggcaattct tctgttttag gtaagatata atcctctggg acacctataa aaggaaatat
     9361 tgtactcatt ataagctcac caacttactt acaacagcaa atttttcgcc tattctaaac
     9421 attattacca tatccccaga ttcaaagtta tctataaaag gattttttac ttcatgctgg
     9481 tgttcttgat ttgtttctgt gtcaaatgtt tctagctgtc tatcaatcat ccaactatct
     9541 atcaaaatat cttctttttc taatatgatg ttatttgtct ctatttttaa atctggtaat
     9601 ttacttttaa tttttccaat aaaaaaagaa ggttgattat gatactttcc ttcttctctt
     9661 attattccta taaattcatt tattggatta gccattatat caccatcctt ttataatact
     9721 ctcctagctg tattaaaatc tcttctacta cttaacttac ttattttaac tacatcacct
     9781 ttttgtggag catgtaaaaa ttctccatta cctatatata atccaacatg gctgactgga
     9841 ttatgaaaaa atactaaatc tcctgcttga agattttttt gttctacttt cttgcctttt
     9901 ttagattgtt gatttgacgt tcttggcaaa ctaacattaa cttttttaaa acagtagacc
     9961 attaaaccag aacagtcaaa actacttggt ccatttccac cccatttata aggtttacct
    10021 agatgttttt ttgcctctga tattactatt ttagctttat cagatacatt accagaataa
    10081 ccaccaataa taatttttcc ttttcttctt ccaaatttac tagcttcttc aacatttcca
    10141 aataaaatat ctatatggta tgttccattt ttttctatag ttattgcagg tcctacgtca
    10201 tttactttat agactccatc acgaccagaa actcctgtaa ttacctgtat ttcatctcca
    10261 tagttcaata aaggatgttt atttaaaaac tcttttgtat aataagtttg ctcatattta
    10321 ccaaccatag gagcagcgca agttttttta gatgggtcaa gttttttctt tctacaatct
    10381 gtatctccac cttcttcttt tctaggacag taagctgtaa attctgctgt aaactctttt
    10441 cctcctgcat aatcttctcc ccctaaatta ctttcttcct taggttcatc ctgtcctgct
    10501 gacttttcat ccataagatt ttgaaaatta agttcaagct caatttgata ttctccattt
    10561 tgccaagtat gtttgtctgt atctatataa aatagtccta caagctttgt ataagaatct
    10621 ttaactttta ctcctctacc agttatacaa cttacatctc cataaccttt aagagaacag
    10681 cttttttcta tcccattaaa ctcgctatca atatctacat cttgattttc ttgttgctga
    10741 attacttttt gcattattac atttacttcc ttaaaaattt cattatctat tttttcgcta
    10801 atcttgcttc catactggtc taccactatt actttatttt ttacattttc catgctctcc
    10861 gaaaaggtgg tatttataat attaaatccc tcttcaaaca taacacttaa tgtaacagtt
    10921 cctttttcaa taacattaaa cttatctaaa ttagcctcta tcatatactt tttctttgtc
    10981 tttttacttg cctctgtata tgcactcatt attgtgtcat aaccatttac tcctataaac
    11041 atctttgtat acttgacatt ggtctttgct attgttccaa ctgaaagcct attttcagca
    11101 aatacttgct ttgctatgtc ttcaactaac ttatctttaa aattgtatga cacttcactt
    11161 tgtgtaagta aaaatcccat atctttagat acaaaactaa tttcattact gcttgaatct
    11221 ttagacctat taattatcat tcctctaaag agttctttct catctacata gaaacagact
    11281 gtactagcta ttggtatatc gatttgttgg aaatttacat caaatgatga ttgtattatt
    11341 gaaaactcta gtgtccttga tggagattta taatcacctg accatgatac cttgtctact
    11401 atgtcagtta tgtcatatat acttccgttt tttatgtgta cccaaatctt aataataaaa
    11461 aatcacctgc cttttcattt aattctatca gcagttactt acaacttatt atctataata
    11521 gttattgttt tggagttact tttggtatct ttattctttt atactctttt aaatctaatg
    11581 taaaatatac atctcgagtc ccatctcttt cttcataact aaacccttca attatgactt
    11641 ccatatttat atttgtttcc gtaattgtaa atcttaatat aaaaccttcc ttcatccatt
    11701 tttctatctt atttacacaa tcatatggtg atggaaaacc tgtatagtca cagaaagtgt
    11761 agtctttatt tggaaaaaaa cttgatattg aaatgctttt aagagctaca ccaccaaaag
    11821 ttgctacttc acctattttg agtatactag aagagtttac agcagctttt ccattaatac
    11881 taaaggaaga tggaaatact ggaaatctaa atctatcttc tgcttgtcta agccacattt
    11941 ccattataag acacccccta actgtgccaa ttggatactt gcaactaatt ttgatgttat
    12001 cttttctata tcagcttctt ctctaatcac tattgtatca gctaacttag ctatgtttat
    12061 atttccacca ttttttctgc ttctatattg atttgcttct tgtttagtta aaaccatttc
    12121 accttcatgg agtctggttt gatagtcatt ataaggaaca tagttcaatc caaatgcatt
    12181 attacctttc ttacttgaat aagctttacc agaatcacca gcctttgttc cagcatatgg
    12241 attatctttg taattttttt tactcttttc cactagattt aaaactgttc ctgtgatgct
    12301 tccatttttt atcttattaa tcatagtttg ccaccatttg catatatctt taactatttt
    12361 acatacttta tctaatatgt taaaaaaagc tcccaatatt ggctctataa ttttccatcc
    12421 agtttccaac agaggtccta aggtctccca tacagttttc caaataacac ccagtgtctc
    12481 tataaaattg tttatttgag gtgcatattc acctaaaaaa gtaaaaattc ttgtgataat
    12541 aggactaaca gcttcaacta caggtccgat tagttggaat gctgtagtta ctattgtacc
    12601 aaatatactt attaatcctt gtgcaattgg tgatttattg tttatactat ctagtaaatt
    12661 atttactaag tttactactg gaagtagtga atctattaat gctgtaaaat caatattttg
    12721 taatagtgta cttcctgcgt taacaattgc ttttggtgca ttccctatca ttgttgctgc
    12781 atgattttca aaacgttctt gcttattttc aaccatcatt ttacgctttt cagcctcatt
    12841 agaaattcga tattgagtag aatcagaaaa ctcttttcca ttttttacac ttctagattg
    12901 catctggtca tatttgttgc caataattat cttaccattc attacattgc ctattttacc
    12961 agccattttt tcagataagc cagctaaatt ccctaatcca ctttctataa aagtttttat
    13021 tttctttagt ccttgaccta aaatatcatc aaaaggttta acaatactct tcaaaccatt
    13081 gttaatgctt gtttgagctc ctgatattat acctggtatt gtattactca tttctttagc
    13141 taaccccatc tgctttacac ttatgccttc caatgcttca tcaaaagttt tataattttc
    13201 tcctaccaga gtattaagag attccatatt tccttttgct gcacttaaga aagctgctga
    13261 tacatcttgt tcactacttg tattcatatt taaagctcta acatctacca tagcttgagt
    13321 tatatctttt tcttttttgc caccagtaat attactaatc attttagctt tagttcccat
    13381 tgcaacaaca tcttcagggt caaaaggcgt atcatttgcc atatttacta aactgtcata
    13441 atacttatca acatcttttt ggctagtttt tccagtattt cttttcatta cactttctag
    13501 aacaactttc tgattttgtt cctgaccaag taagtcaaca ccctttttca cagcgacacc
    13561 tatagtagca ccatttgtca aatcagatat cttccctttt atcgaaccaa gtattccact
    13621 tgccatatcc tttacagcta ttgcaccact ccatacctta cctgcaaaca atctcaattt
    13681 gggaaatact ttacttataa tgctacttgc catatctttt atagctatta caccactcca
    13741 aaaagtattt ttaaacaaca ttaacttagg caaaattcta gccatagcac tacttaccat
    13801 atctttaaga ttaatacttc cttgataagg acgtttctct actcgtttta attttcccaa
    13861 gatactactt atcactctct tagtcatatc ttttgctttt attacgatat tccaaatctt
    13921 gccaccaatc ttttttaaat ttccaagtaa tttattttta gttttttcta cttcatcact
    13981 tttagcttta aatattaata taatcataat atttgaatta aactcatttt tcttttgaat
    14041 caacttatta aaataatcaa taatcatagc tatagttatt gctagtctca taatacttcc
    14101 atttaaactt ccaataactc ctgaaacagc atttatgcta gaacttaatt ttgccatagt
    14161 tgaacttaca ctactagcag tagtttgaac ttttcttaac gaggaattta aactgtttac
    14221 tctactactt gcacttccaa tagaattttt tatgctacta gccattacat tagtggaaag
    14281 tgaaacagcc acagttgctc tcctaaaatt atcagttact gatataattt ggttcatttt
    14341 tggtgtgtaa ttatctgtaa gatacatatt tataccttct tctctcacat ttcccattac
    14401 ataccacctc ctgtcaaagg acacactcca tatccatgtt tcattttttc aacttcttcc
    14461 tcaatttcta aggcgaaaaa agcttcaata agctttaatt cgcctttatc cattgcataa
    14521 aaaagggacg gtcttatacc ttttttcttc caataatagt acattattgt agtcttggta
    14581 tccgtcccta ttagtttttt acctcttcta tcactgcatt tttaccataa cccataacat
    14641 cttgaagtat tctatatagg ttggtaattt cacctggtaa taatagtttt cttgccaatt
    14701 cttttggtgt tggagcctta aatttatcca ttagttcctt atttttaaat aaaagctttc
    14761 catccaaatc acaaacacct tctataattg taagcatctg catctccggt aagtctatat
    14821 ccgttttcat attttcatca attttaagac aagtattttg tatctcttca tctttactta
    14881 ttgttaaagc tctacacatt actttaaatt cctgcccaaa tatagttgat aatctcttaa
    14941 gttcaactat agtacttggt ctttctaagt tttctgtatc tgcatttaaa agtaaatcta
    15001 ctatgtttat attttttgat aatccatttt cattcattat tatttgctcc tttatcaatc
    15061 aaattcaatc aactaacttt tttacttatt aactacctaa atttattaag tatatatcta
    15121 ctaaatatca gtataaaacc aaatataaaa ctaaattata tcaagaaaat catactcagt
    15181 aaatgtgaaa ggtgcttcta tctctccttt tactccaacc tcccaatcag ctaaagttaa
    15241 atcatcaaat gctatatttt ttactgctat tctttctgct ccataagaat ctgggtcatt
    15301 taatttagat atagcaacaa atctaggttc agaacctctc tttatctttt ctccaattaa
    15361 cttgtgcatt cttgaactaa catggtatag agttattgaa ccttttcctt tatatcccat
    15421 atactttgta tcagtaccca tttgacctgc tattataata tcctcttttg taaattccat
    15481 ctttgcttga aacttcttta cttctgctac tttgtttcca tcaagccaaa gttctcccca
    15541 agtaccactc attacatttc tagcttccat atttgccata ttaataatcc tcctatattt
    15601 ctattgataa atctatatct tccatagcat caagtacttt tatttttgct tttaaaaata
    15661 ctttagaacc tgtgttagct tcttttattt cttgtaatgt catataagat aaatctactc
    15721 cttttgattt taaatacgat ttttgtgctt caaaatctat ttcaacagta gaatcagatt
    15781 ctataagtgc tgacttttct aattcttcta aataactttt tatagctact atcaataaac
    15841 atttgttgtc ataactgtta gtaacctttc ctatatagtc atctattatc acctttctta
    15901 tgtcactatg tataatatct aaagtgtcaa ctatttttat tttctggaac atttctcctt
    15961 tttcttctgt taactcagtt aaagaattta ctcctcttgc aattcttata gcccctgctt
    16021 ccttaataag tataagctct cctttattaa cccttgattc tgcatcaact ttcgtcatct
    16081 taggtatatc aactacatca ctaagcttag tataagttac tgattgactt aaaggtgttc
    16141 ctgctataag tccagccacc ctacttgtaa actcatcaac actgtatttc ttttctccaa
    16201 ctaacacatc ttctgtagta aaattaatta tcccttcatg atttcctaca acttttccta
    16261 atacagcttt aaccttaacc ttatctatat ctctaagttt aattatccaa ttttttatag
    16321 cagtcttatc agcttctact gcttttggca tacatagata attaaattcc ttagtctcta
    16381 aaaaatctaa tgcagtttga atatctgctt ctccttctat tacataaact aataatttat
    16441 ttggagtgtt aacatttccc atcaaagcta aatttatata ctctttattt tcagcagata
    16501 aatcaactgg tatatcctct ttttcatgta tttcattaag acctagtgcc ttagcatctt
    16561 taagcaccat tgcaattatt cctctagctg aacgttcttt aacagttgta gctagctcct
    16621 taaatgatat gttgatactt ggtaatccta tagccatact atcactctcc taaatttaaa
    16681 ttgatttctt ccatattttc atatactaca tcttcttcat atacactatc cagataattt
    16741 agtgtaattg taaagttcaa tacataaatt gactcttctt ttttaatact ttgttctagc
    16801 tttgttatat ttaattttct gtctgttacc tttatatttc tgttgaataa cttatttaat
    16861 tcatctgaaa tttcatacat tctaatactc tttgactgtg gttttggtaa gtattttata
    16921 gagataacaa tatctttatt attcgtattt aacattatag gtgtacaaat aactgatagt
    16981 atacttacaa aaaaacaaga cccttcagag ccttgcacac tttcatcatc aataaaaata
    17041 ctttcagtaa agtttttttt gagaatctca ataattgttt ctaatatttc cttgtattta
    17101 agcaaataat caccacacta tcactaaata ttcttgctta atctgataat cttactataa
    17161 atgatataag tcatttttaa atatacatct catttattcc tgtcaaatat cttctatatt
    17221 tttaggtaat attattcttt tcaatttatc acctccttca atatggattt ctttcacaat
    17281 aacattatct cacatatttt attgcctagt gttgcaatag tgtatataaa gtgttgtaaa
    17341 gttgtcatat tttttataat atctcactca atttaaatat catttttttc tttaaattgt
    17401 agcaagatgt tctatctaag tgtagtttca tagaaatata tgtcatattg ttttttgtct
    17461 tactattata aaaaagatta aaaaattcca tttctatatc atttagacat gttagagcat
    17521 tttctatctt ctctttttct atttctatat cttctttttc catttttaat tttcttaatc
    17581 tttcctctct ttttaacacc tcattttcca cagaagaact tatgttatat gtgttactag
    17641 tcttttctgt atataccatt gctccacatc cagagtattc attctcacat ctttttatgt
    17701 ccaaattaat attctttatc tttatatcca aatacttata attatgtaat ctttcatctg
    17761 tttctttaaa taatatatct tttttgtttt ccatagtact tctccttttt tatatactta
    17821 ttattagctt caaatttaca ataatctctt tagtgcacag ttttttacta atctcacaac
    17881 tttcaagaac atattgttct ataactgtat aaaaaaaact tcttctatat tttgttctaa
    17941 aatactagaa atcttcttag ccataagaag accaggattt tttatacctc tttctatttg
    18001 agatatatag tcatttgtaa ttccagcctt tctggcaagt tgaatttgtg tcataccttt
    18061 gcattttcta taatatttca agttattttt aaacattgtt ttatacctcc attaccatta
    18121 agaatatttt gttcttccac acttagatta tatagaatca tttgttcttt atcaatagat
    18181 tttagaaaaa aatattcttt tatattttat tagttttatt ttttagaaca atttgttata
    18241 atctatacat aaggtggtgt tataatgttt gccaaaagat taagagaact tagaaaggaa
    18301 tttggattga ctcaaagaga acttggagaa aaagtaggcg tttcccaaag agtcttaggg
    18361 tactatgaaa cagaaaacag atttcctgat gaacacattt taaacaaatt ggctgatgta
    18421 tttaatgtat cagttgatta ccttcttgga agaaccttag ttaaggaaaa tattgataca
    18481 gtagctgcac atagaaaaaa tccacatgaa gagttacctg aagaagcaca agaacaactt
    18541 aatgattata tagaattttt actaaataaa tataaaaaaa aataaaccat atgatgagca
    18601 gtctattttt ctgctctttt aatataattt aaaggcaaac atacattcta ttttaggggg
    18661 atttttatga ataatttgga taagcttttt gaattagctt ctcaagaaga gataataatt
    18721 cattatacca cttatattgc aggtgattta gaagggctat atataaacaa acatggtatt
    18781 aaaattatat cattactcag taatttaaaa caaaactcaa aaaaattgac atccatctta
    18841 gctgaagaac ttggacatca tttcactagc cttggatact atgtatcctc ttataacgat
    18901 tattatacaa aaattattat agataagtgc gaaaataaag ccttaaaatg ggcttgtgag
    18961 ttcttaatta ctgaagaaga tataataaac ataattaatt ctggcatcac ttgcgtttat
    19021 gaaatggcag acatacttaa tgtggatatt actttctttc aaaaaagatt agaattttta
    19081 tcattaaaaa aacagtcttt acaacttgga aataataaat atttaatatt aactaatttg
    19141 ccgtatttct acatatttga tccaatttca taataaaaat agtagtaatt ttaactggct
    19201 taacagtaaa atctatatat ttattttaac aaattttggt ttctataaat tcaatattaa
    19261 tatataataa tgatatcata ttatttaaaa ttctaaaaac aaatatatgc ttttgttaag
    19321 aaaggttgtt tgatgaatgt ataaagaaaa gttgtttgat aattatttta agttcttagc
    19381 actattattt tggccaatta tgtggtataa gtggatagta atttcaaatg gtactcttga
    19441 aaatatgtta tttaccatat atgcaatcgt cgcaattatt ttcatcattt tatattcggt
    19501 gtttatgata aaatataaag acattacaca aatagatttc ttttacagga taagtactct
    19561 acttgctttt atatttacac tttttagctt tttaatatac ccaaagagtt tatttttctt
    19621 gtatttaaag attattttta ctggaatata cctttattat tcaatagtaa aaactcttaa
    19681 atttaaagat gatgaaggtg tagtgggtat tatgagctca cttttattaa ttgtaataac
    19741 tttgttctac taaatttaat tctatttgac ttaatatggc tttaaatatt agacatctac
    19801 attattttct gatattattt aagatggtag tcttatatta ataatgaaat acactatgaa
    19861 ggagttaact attatggaaa aaaaagtttt agcaacagtt ggagaaaaag agataactaa
    19921 tattgatatt gaaaatgcat taaaatcatt agacccttat caagctatgc aatttaaaac
    19981 tgaagaagga aaaaaacact tattaaatga tttagtgaat caagaattat tcttcttaga
    20041 tgctaaagaa gaaaaattag atgaagaaga aatatttaaa ttagaaatga aaaagataga
    20101 agaaaatgta ttaaagcaat ttgctataaa taaagtttta tctagtgtta atgtaacaga
    20161 agatgaaaaa gtaaaattct ttgaagctaa taagagctct ttctctaaac cagaaagtgc
    20221 aactgctaaa catatattag tagatagtga tgaaaaagct aaagaaatac ttgctcaaat
    20281 aaaatcagaa gaaatatctt ttgaagatgc agctcttaaa cattcttctt gcccatctaa
    20341 agatatgggt ggtgatttag gaacattcgg tagaggacaa atggttcctg aatttgaaga
    20401 agctgtgttc tctatggcta aaggtgaagt aagtgaacca gttaaaactc aatttggata
    20461 ccatataata aaattagaag accttcaaga aagtacagag tctacatttg atgaagttaa
    20521 ggctgaagtt gaaaaaagtt tattatatca aaaacaaaat gaagtttatg gaaataagat
    20581 aaatgctctt aatgctaagt acggaaacct tgtaaaatac aacgattaat cataaggtca
    20641 tatataaaaa agctgtcttt tcacaatatt tattagaaaa gacagctttt tatattaaag
    20701 tttaaatatt cagtttcata ttatcattct tttattgtaa agaactcagc ttaaaagttt
    20761 aaataagatt tttaattatc attagctaaa tttttatatc cacgtattat ctcataacag
    20821 taacctatta catctcttct ttttattatc ccaataaatg tgtcttggtc atctataaca
    20881 ggaatgaaat tttgatttaa agattttata actaaatctt caatatctgc gttgatggaa
    20941 acaggagaat tatccatttt tctatttata tctgtcactg gaacatcttc caatcccttt
    21001 aagtccagat ttaaatcgtt ttttaatgtc cataaaaaat caccttctgt tatagttcca
    21061 acatatttac catccttact tattattgga attgcagagt atttgtgata ttccattttt
    21121 tctagtgctt gccttatagt gtagtcttca tatatatatg ctacttcact ttttggtgtt
    21181 agaaagaata atatgttcat tacactaccc accgttactg aaaacttata aatattactc
    21241 aatatttata atgttacttt tttgcagtaa ctttctcctt tcttattttc taagtattct
    21301 taatactata cgctgtaaca agcgtttttc ctttgctcat tttaatctta ttatgcttta
    21361 ttaatatttt tatatggtct atcagtatca cttattctct ttttagaaaa tttactaaaa
    21421 attaattgac ttaaacttag ccataataat tttactctac actattaatt ttaacatatt
    21481 tatctcatta ttaatgattt tattttaata attttaattt attttgctat taattttatt
    21541 tacaaaaact gaaaaaaccg ctaaatgatt agcggcttat ctctcatatt actagaacac
    21601 taatatatat gaatttttaa ttataatcta actacgttag cagcttgagg acctttagct
    21661 ccatctacta cttcgaactc aacgctttgt ccttcttcta atgttttgta tccatctcca
    21721 gttatagctg agaaatgtac gaaaacatct ccttcacctt ctacagatat gaatccaaat
    21781 cctttttcgt tgttaaacca tttaactaca ccagttttca ttaataaatc ctcctaaaaa
    21841 aacacaaata tttagctact cttttctgta accttattac tactctatca tatatagcta
    21901 tgtttgtcaa ttattattat ttttatacat taataatcaa tttttttact ttataatttt
    21961 aaatgattat tatcttttac actttatttt tctttataat tttttaataa tttattttta
    22021 ttatatggaa tcttatggac ttatcactcc agcctaaatc ccatggcacc aacattctat
    22081 cagtattatg gcaagttacc aaaggatatc ctcttgagtc taatcctgtc actgtcgaaa
    22141 catgtgttat tctgcctttc ttctcataac caacaaaatc ccccggacgt aaattataag
    22201 cttctttgta tacttcttca taggaacctt tagcaattaa tgaacctctt ccactataca
    22261 gtgcataatt tttaaaagct tgtgcattta cccatgcttt tgtacctgct ctctcgctat
    22321 aattccaaat tgagttcttt ttgaaccttc cactttcaaa cattatctgt gatgcaaagt
    22381 ttgcacaatc tccaccttct ggattataat ccctatagtt tggattaaat ttcataccat
    22441 gttcttcatc agctgcggct ccacagtatt tatgtgcata atctatagcc tttttttgtt
    22501 catctgtaag ctgtaactct acaccatctt gttgcccaat atagtttctt atatcctcag
    22561 attttatatt ttctaaattt aaagagtctg caaaagggtc tgtgtaccac tctttagtta
    22621 tgacatattg attatctata atttttacat ttagataatg atatgtacct attctaaagg
    22681 cattttcttt gtccttttga ttttcatatg aatacttata ttcagtcgat actgaacaga
    22741 ttataccata gaggtctttt tctttttctt ttattttttt aatcttaacc cttgtcttta
    22801 tgtctttaaa ttttacacct tgcttactag accagttttc taaatacttc atctttttta
    22861 cttcattttc ataagcccac agaccaaatt ttttatccgt ctcataaaga ccttttaaaa
    22921 tgttttcatt tttctctaat attgccttat ttctgtagtc aaataagttt tcaagcaata
    22981 gtttatattg gtcttttaca tcatcacctt caacatttgc agattccatg actttttctt
    23041 ccatattttt catacttaaa tcattgatta aaaatacacc tgctgtagta gctaaagaca
    23101 aaaatacaaa tgccattata cctttgatgt acttattctt atttttcagt attttcaaag
    23161 ctacacctcc caaatagtac accttataaa tgaaattaca aagaagattc tgtcatatac
    23221 aataattttc atatttaata attctctatt ttattatgtc caatattaaa aattaattgt
    23281 atcaatacac tgtttttttt acaatttttt attttataat ctactttttt ctttttttta
    23341 tagtaatata tatgtatata aatattttgg tatcattata attaatacat actaatggag
    23401 ggttttttat gcaaaaaaaa gtcgcagcaa ttaatgactt atctggaata ggaaaatgct
    23461 cattatcggt tgctatacct atactttcag cattaaaggt tcaatgttgt ccatttccaa
    23521 cggccatact ttcaagtcaa actggttatc ctgaatttac tttcttagac tttactgatg
    23581 aaatggtaaa atattcaaat gtttggaaaa atttaaaagt caattttgat agtatatata
    23641 gtggtttttt gggttcaaag catcaaattg agatagtggc taattttata aatgattatc
    23701 ccaatgcttt tatcgtagtt gaccctgtga tgggtgataa tggagttatg tatccaatat
    23761 tcacagaaga aatgagacag gaaattaaag aattagttaa acattcagat ttaactactc
    23821 ctaatttaac agaagcttgt ttcttaactg gaaatgatta tactaaaagc gattataata
    23881 gagatgagct tatatatatt gcaaaatctg tatctgattt gggacctagc aaagtcgtta
    23941 ttactggtat attagaagat gataatatat taaatttggc ttatgataga gataatgacc
    24001 atgtattctt tacatcagtc aaatacaata attgttctta cagtggaact ggagatatat
    24061 tcacatctat actttgtggt atgttagtaa ataaacatga tttgggagta gcggttaata
    24121 ctgcaacaga ttttatatat aaaactataa attacacatc tcaatttgat actgatagaa
    24181 atgatggagt tatgtttgaa aactttttat cagatttaac taatatataa aatcaaaaaa
    24241 actatccatt ttataaatac ctataaatca taaaatttag gtatttacaa aatggatatt
    24301 atttactaaa ctattggtat ttcaattgtt accatagcac caccagtttt cacagaattt
    24361 tcaatatata aaattccatt atgtttttgt acaatattat ttacaatata aagtcccatt
    24421 ccgtaatgag atttagaatt tctactcaaa tctcctgtat aaaactgttc tgtagctttt
    24481 gacaaatctg ctttagaaaa accacttcca caatctgtaa ctacaaattt taagtattta
    24541 ttacttactg atacacttat gtatagttta ctctggcttg gtgaataatc tatagcattt
    24601 gaaattatat tcataatagc tctgaataac agttctctat ctatagttat atttttaggc
    24661 aaattatctt gtttataaaa ctccacatct aagtttttag tactagctaa tgcttctatt
    24721 tgcagtaaga catcttttat aaactcactc atattaattg cttctttatt taatatatag
    24781 ccattctctg tttttgatat atctattaaa gtattaatat atttttctat ttgagtgaca
    24841 ccctttgaga tatattccat gtattctaat aactcaggat tttcatttat ttcaattaat
    24901 aagtcagtat ttccatgtat aattgttaat ggtgttttta tatcatgtgc cagtgcagat
    24961 atctgttcct ttctattttc ttctagcatc cattgttttt ctaaagaatt ctttaacgcc
    25021 aacttcattt tatccataga aaaaaggata ttgttaattt cataaatttt actagattct
    25081 acaacaaaat ctaaatcctg ttgttctatt ttttctgtag tattttttaa aagctccatt
    25141 tcaatattaa atttcttgcc aaatactttt gataatataa caatttcaat aaaaaatatt
    25201 atactaaaaa taatcatacc taacatctct ggttttggta aatatgctct taaaactgga
    25261 gaactatact ctgatactag tttgtatttt attataaaaa cttcatcttt cttaaaaaac
    25321 tttatataac tttctgaata tgcatgcctt tcttcaatat ctctcattaa attccatact
    25381 tgttttgatt cttttttatt aaaagtgcct gagatcaaat tcccatctaa agtataaaca
    25441 ccatactcgt ataaatttgg aattatatct tcaattgctt tttcacttga aataatcttt
    25501 tctttatatt tatcaaattg tttttctgaa taattagctg gtaatattac acctgatgat
    25561 aataatacag aaaatgataa cattaaaaga agtgcaaatg aaattgtcat aacaaagaat
    25621 aaagataaat attttataaa aatagttctt aacttttcaa cttttttgtt gttagatttt
    25681 ttacttctaa gcatttgtgc catatcctcc aaatatttta attaattcat aattaatacc
    25741 taaccagttt ttatttgatt attttatatt tttcattgct aagttatatt ttaacataat
    25801 aatataaata taatgtaaat aaacagtcaa tttttattta ttcttctgac tttttacctt
    25861 cccatttaga aaaccaaata cataaaatta caaatgctaa aattgttaca aaaccacaca
    25921 ttaaacctgt tgattgatat gcttttattg attcaacctt tatatattca accgttttag
    25981 aacttgaaaa agacgaccaa atagttataa atctaacacc ccatgcacaa ggaaaatact
    26041 gccaaatgcc agaacccaat ccagttaata ataatgctga aagtaacatt tcaactatac
    26101 ctactccaat tgatgttcct tttccgaacc ttaaacttaa gaataaatgt aatatgtatt
    26161 caaataaatt acacccaatt aaaattagaa ttgaaatcat ataaaagctt gatgtaaagt
    26221 aagccttaga tataccaaat ttaaaaccta atacagacac taatgttgct acaaaaccaa
    26281 gacatactaa gattatatat ttacttataa gagtaagata ttttggatta gatgttgtta
    26341 acatatgttt ataattccct gcataatatt cttgttcaac aaacattgaa cacacaacac
    26401 ctgacaataa aggaaaagct attgctataa cttgtaaata tgctactatc tttaatatat
    26461 tatcagctcc tgaaacctta taataccata aaaacaatcc catccccatt aaagcaatgc
    26521 aaatgtgcat tagtattata aaatttctct ttaattttat taaatcagaa caaaatattc
    26581 ttattagttg tctcatatta cttcacctct ctattattga accaagctct tgtaactaaa
    26641 gcaaataata caaataaaat aacagatatt aatactcctg gtaaaataac agcattattt
    26701 aaaagttcac tcccctctgg tacaggaagc ccatttggta gaatatgcaa tacaacaacc
    26761 attaatcttg aaggaatcga atatggaaat acataccaca tttctgtagt tgagaaaaca
    26821 gcaactccaa caatagtaaa gaacatattt gttagaactg taacaaatat accaaatttt
    26881 gaagctaaaa acatacaaag aggaatctgc cacataaata gtaaaataat taatatagtt
    26941 gcaatgatat tttgacctat tgaaatagtt tctttataga ttatcccaac aaaaactaca
    27001 cctattgcag aaataatcga tgctatagaa caacaaataa tacagtaaat catttttcca
    27061 gcccaaacgt ctttcatttc aattggcaat ggtaaaatac cacgatattt acttttttca
    27121 tcttttaata ctattaaaga agatattaat gttaatgctc ctggaagaaa tgtcgtatac
    27181 caccagttga aagcagcatt ttgccctcca cctagtccac acaaaagtat acaaaacact
    27241 ggaactatcc atatcaactt cattccaaaa gttcctttca tcttttgaaa ttccactcta
    27301 ataaatcttt ccatattact cccctctctt tctattttca gctacaactt tcataaaaag
    27361 tttttctaag ttttgttctt ttgatacttc tccttgataa ccaattactc cattagaaat
    27421 tattccgatt tcatctacga cttgttcaac ttcagacaag atatggcttg ataaaataac
    27481 tgtaattcct tgttttggaa aagaacgaat caattttcta agttcttcaa taccaatagg
    27541 gtcaagacca tttgtaggct cgtctaagat taaaagtttt gggtgattta ataatgcaat
    27601 agcaatacca agacgttgct tcatacctag agagaattga cctgaacgct ttttaaaagt
    27661 atctcttaag tcgacagtct ctaaaacttc ttcaattcga gagtcaggaa gaccaagtaa
    27721 agtagtatgg acttttaaat tttctcgtgc tgttaaattc tcatatatag caggagactc
    27781 aataagagaa ccaatatttg ataaatcctt tctagtccat ctatgtccct caaacattat
    27841 ttttcctgaa gttggattta aaatcccagt tatcattttt aataaagtag atttcccagc
    27901 tccatttggt cctaataatc catatataga attagattta attgctagtg aaatattatc
    27961 aactgccatc tgctccttaa attttttaga aactcctttt acctgcaaaa tataattatt
    28021 cataattttc atcctttctt ttgtcaaact tatttacaag aatcattcta aatcataaat
    28081 ataaggattt tataagaatt aaaaaacaaa aaaaagatgt gttaaaaaca catctttttt
    28141 tatcttaatt ctattaattt tccattgtag tatatctcta cttcttcaat ccaaatgcta
    28201 ttatcttctt cttggatgac atttttaata atattattta tagtatatat tatattttgt
    28261 tcattcaaat aatcatcttt ccaagaaact ctactttttt tatctggttc tatcaagttt
    28321 attttaacaa gattatcttt ttccttaact tctacttcca tgtctaaacc attaaaatac
    28381 tcatttgata atgtatctaa gattaatcgc agtttatcct taagtgaact ttcttcatca
    28441 atctgtaagt actttactat tttctcatcc tttgaaatta tctctatagg tattttagta
    28501 gtttttttag aattaatcat tttactagtt gtttcttctt ttgcatcttc atccttcttt
    28561 acaactgctg attctttaga atcaactagc tcaactgcac tgatgctata atccatacat
    28621 gctgtactca ataaagtagt agcaattatt aaaaaactta ctgctatttt attcataagt
    28681 atccctctcc taattctctt tcgatactta ttataccata gtatagatta tatttttata
    28741 cttttaaatc aatttataaa ttattatagt aaattattta caattctaag ctatttgaat
    28801 ttatttttat agtatattct ttctctaaat tcgacagatt tttattcaaa agccctttta
    28861 tttccctcgt atttctatac ttgttgctat gttcttcact atgttcataa tcatatgaat
    28921 cattgtcata ttcttcatca ttattaaatt cttcaattgc ttcttttgac tttatatcac
    28981 aattaaattt atacttattt actggtatat caagtcttac cttagaattt ggaacttcta
    29041 tgtccaggaa cttagcaatt ggcatattag agtacaagta aaattcactt tcataatttg
    29101 aatttctacc tattttctct tttatattta cattttcagg tatatactct tctatattat
    29161 tgataaatac atcttcattc tctgaagata tatccaatga ctctgattct atgtttacat
    29221 ttttaacgcc taaaattgat tttgttgaca agtgaagatt gctattagat attaagttta
    29281 ggttttctaa tctatttact ttgttctctg ttatagctct actaaaacct ccataagaag
    29341 ttttatatgt tatagtattt gaagctacat tatcatatac aaataagtat gaacttgtac
    29401 ttgatgcatt tatgtctaca ttatttggta catatattat aatttctcta taatcatgtg
    29461 aaaacatact atttatagat gtatttaata attggtcgaa atttttaatc tttttaattt
    29521 tattttcata ttttctttca ccttttacaa ctaaagtctt atcttttaaa gttacattgt
    29581 atttgtaaaa ctcattattt cctcttgttg ttacacgcac atcttcactt gaacttttct
    29641 ttattgtaac gtttatattg tcaacagata tatctaactt gtcaatattt actttttctt
    29701 gataaagttt attttctttt ttaaattcag cttccttttc taaaccaaag tttattaggc
    29761 ttggcataac taaaatccct gaccatgttg tccctattat acctattacc aataaaactg
    29821 cagcaaatat agcaagtttt ttattcatgt ttatcttctc ctttcttatt ttctatatct
    29881 tcaaggtcct tatatatcgc ctcaaaattt atactatcat cattatattc atcagtttct
    29941 tttatgcttt cttcttctct attgtagtaa tcattattat atgaatcttc attaatgtct
    30001 ttattcaaat cttcatcatt taaagtagct ttttttcttt tttccatctt ttcatttgcc
    30061 tttatataaa tattttttct ctttatccaa tgtatataat tatttagtaa ttgtttacct
    30121 agttttatag caaataataa aatttgccat gcaagtatct caagccctac aaatgctatg
    30181 gttgcgaaaa tacaaaacat cataacatct ggaaccattg ctataaattt aatcacaaat
    30241 ggaattaata taacaaaagc aattaatgaa catactaatg caatagtaac agatacaata
    30301 gcacatacaa acgcaaatgc tggaacaatt agaaataaag ataacactac taataatccc
    30361 tttataactt gtctagataa tccccctaca ccttcttcta tacttggtgt aagtttatca
    30421 tttatatttt cttttgattt attgtacttt tccttagctt catctttcaa gatagagtaa
    30481 tcctttttta tatttgattt aaacctacta aatttaccat ttaatatatc tgtattcatt
    30541 atttttccat ctatattttc ttttgattta ctatactttt cttttgcttc atcttttaag
    30601 atagagtaac cttttttcat atctgattta aatttactaa acttatcatt caaaacatct
    30661 gtatttatta ttttttcctc ttcaatattt ttttctttag tctgcaagat aagttctttg
    30721 gctatagatt ttggtgaacc aagtgatgct attatctcca tatcactctt tccctctata
    30781 agaccatcta cgaaatactc ttcataatct ctcaatatat cattaacttc gctctcagaa
    30841 aaatcttttt ttagataatc ctttaaaatt tcaagaaact cagttttatt caagttcatt
    30901 acctccctca tcttcactta ttaaaatctc aacagccttt acaaattctc tccactcttc
    30961 catcagattg gataaattct cttttcctag tgaagttatt ctatagtatt ttctagcagg
    31021 accttcatta gattctagta tatatgtttc gaaatacaaa tcttttgtaa gtcttcttag
    31081 aataggatat attgttcctt catttacatc tattacttta gagatgttat gtactatctc
    31141 atatccatac atatctttct tagatatgag agcaagtaca caaatttcta aaacaccctt
    31201 tctaaattgt gtgttcatat actccatcct ttctttaaat acacagtatt gtgttttaca
    31261 aggtactatg ttaaaacttc ttatatcctc ataataacac agtactatgt tctgcacaat
    31321 agttattttt aaatattctt gttgattaaa tccttctata aatgtataat actctaatca
    31381 gatatatttt atgaaagtgg tgataaattg gctaaaatga aagatgataa aacaatatta
    31441 atcttaactg ctcaatttgg tgctggacat ataagtgctg caaaagcagt caaagaatgt
    31501 attattgaaa aatatagtaa ctataatgtt gttatacaaa actttataaa tgcaagtatt
    31561 cccatgatga ataaacctat ggtaaaactt tatgaaaaca atacaaaata tactcctgga
    31621 ttatataatt actattatta ctttaagaaa tcgtttgatt caagacatga tttatctcat
    31681 aaattatata cacctaaact ttctgagtat attgctgata taaatccaga tttgattatc
    31741 tctacatttc cactagcggc tgcttgtgta aataacttta agataaaaaa tcctgatata
    31801 aatataccta cacttacagt tataacagat gttgtagata gtatggaatg ggtttttgaa
    31861 aatacagatt tgtattttgt tccttctcct gaaataaaaa atagattttt tcagaaagga
    31921 ataaatccag actccataaa ggttactgga gttcctgttg ataagagatt tcaaattgaa
    31981 agtaaagaac tatgttgtga taaatataga ctattacttc taggtggagg aagaggccta
    32041 tttgatatag atgaggattt tatgcattgg atagatgaat ttattgaaga acatagtaat
    32101 tctatagaaa ttacaatagt tactggtaaa aacaaaaaat tatatgacaa tctaactcat
    32161 aaaaaacctc taaaaaatat taaagtactt ggatttgtaa acgacatgta taatctaata
    32221 agagaatgtg acctaatgct aactaagcct ggaggagcaa ctatatttga agctattcaa
    32281 tcacaaactc cagtactcgt gaaaatgcca aaagttggac aagaaattga aaacgctaaa
    32341 tttataatag ataagggtct tggcatgatt tatagtgatg acctagacct taaaaacata
    32401 ttttataaat tagtatcaaa tgaatttgat tctataataa actttatgaa aaaaaattta
    32461 gaagaattta aaacagttat tcatccagag aaaattgctg attatatatc agaattaata
    32521 gataatcatt acagttaaat tgtactatta aaaaatttca aacataaaat taaaggcaat
    32581 caagtatgct tttacacttg agaatttact catagtgttt aataatactt gattgccttt
    32641 atcatatcaa aatcaaattg ttatattagt atatattttt cttgtactgc atatacagtt
    32701 gttgtatttc ttccatactt ctctttaatt ctatttgtaa atctgaagca gtctggtaat
    32761 ccttattttc tatagcttct ctcactctag tgaatagtga ttctctagtt aaagtatcct
    32821 tcataatatt gaactttaaa tccgatattg agttccaaac tacctcatca agcgcatcca
    32881 tattttcttt tgtgtgtatc ttgacaatac ttcttatttt ttgaatccca gcctctatta
    32941 ttctagtttt taattttttt tgccacttat ctatagctcc aatcttaaat gattctatta
    33001 tactatcagt aaatacatca ccttgtttta aagattttaa ttttgaagca tatatttcta
    33061 agtttttcat attttcataa actgtagccg gtggtattcc aaatcttgca tttctttctt
    33121 ccaaactata atgttcaaat acatcatttt catctctata tgctctatct ttttctaaat
    33181 agaaactttc ttcacctaca tttttagata actctttttc aagttctttt gtactaagac
    33241 cactcttagc tgctgctttt ataccatcca acattgtttg ataacatcct gctattacta
    33301 aatacgtgtt agatagaggg cttggtgacc ttaactcaaa tcttacagtc tttggatttt
    33361 tcatatctct tattaatcca acaagcactg acctatttct tgatggaatc tcataactat
    33421 gccctaaaga tgtaactatg catacaggag cttcaaatcc aggaactaat ctattaaatc
    33481 catcattaga agctgttaca attgggttta atacttcata attgtaaaga agtcccataa
    33541 gagccccata tcctagttca cttagataat cttcttttaa atcttttgga gcaaatagat
    33601 tttttatact tccatccttt aacttcgcac ttactcctac atgtgtatgt ccaccacttc
    33661 ctgctactcc atgaattggc tttgctttaa atgtaacttc tagtccatgt gatgtaaata
    33721 catcctctat aacatctctt actaataatt cattgtctgc tgcttgtaat ggtgtagaaa
    33781 acttccaaga aacctctaat tgctccatag catggtttgt tcttccatct atactaatag
    33841 aactttgtat tcctccgacc tctttgtgag ccatttcagg gtttacacct agcttttgaa
    33901 gtataattaa acttttctct aaacaagttc ttataatacc atgagttctt ttccagtatt
    33961 gctcttttag gctttgtgat acatataatt tctctaagtc agctttatcc tcaggagtat
    34021 taacccagaa ctcaagttct gtagctgctg ttaacattat ttcctctata tcatctactg
    34081 aatctattcc tatgttattt attacatgtg gatattctct aaatatttca tacattgact
    34141 ttttaaagta cttatctgct ttttgaagta ctcctctaga acatactttt ttattatcat
    34201 gtattaaaaa tgcaggtatc tttaatgttc caacaggtag acctacttct tcgtctatat
    34261 attccatatt gtaatctaca taccaatgac aacttttatc tggcattaaa tctactttcg
    34321 cattatttaa tgtagctata ttataaagct caacacttga accatcagtc tgtatagctg
    34381 attccaaaaa tttatctata tcctccaaaa ataattctac aggaatcttt tcatctgttg
    34441 catttcctcc taaatcaacc cccattaagg aaacaaatct tatattttta ttgcttttta
    34501 ggatttctct taattcttca ttagtgtgtt tatctttctc tattacgtat aatagactat
    34561 ccatttttcc actctcccat aatttttatg tatattttat tttgttaaat acaagataaa
    34621 tttcattttc cttacattca tgaatttttc ttgcaaatta ttcgtgtttt attcgtgttt
    34681 ttatttatat tattatatat caagtataaa aagttcgcaa tattttttat tggaaaaaat
    34741 attatacata caatagttta
//
LOCUS       DAMJWP010000014        34362 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.015, whole genome shotgun sequence.
ACCESSION   DAMJWP010000014 DAMJWP010000000
VERSION     DAMJWP010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34362
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.015"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(43..960)
                     /locus_tag="PC700_000644"
     CDS             complement(43..960)
                     /locus_tag="PC700_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDF2579968.1"
                     /translation="MKNIKMGMIGLGSIAQKAYLPILTKSERFEFVGAFTPNKVKREK
                     ICSDYRIMPFDSIESLAKKCDCIFLHSSTETHYEIIKILLNLGVHVYVDKPLASTVNQ
                     GEELIELSKKKNLNLMVGFNRRFCPMYKEIKNNATEIVSINICKHGLNSLRNVRFDST
                     LIDDYIHVIDTALWLANEDVEISGEDLFLTDNKNLIFVSHKLKGKHFSVNTSMHRDSG
                     TKLEQVEILSKGKIQRVKNLNVLEIEEGGNLTLKQSGAWVNILKQKGFEDISNHFIDC
                     IENNIKPAINGEECIKAQRLLEKIINSVK"
     gene            1422..2591
                     /locus_tag="PC700_000645"
     CDS             1422..2591
                     /locus_tag="PC700_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF2579969.1"
                     /translation="MKMFKLEEQKLKDLGAIITTNEIKQQPELWLETYEIYKSNKEKL
                     NRFIDTISNNHGQFRVIFTGAGTSAYIGNSILPYLKNKNDIRKYIFEAIPTTDIVSNP
                     YDYLKKDIPTLLISFARSGNSPESLAALNLGNKIVDNFYHLAITCNPQGELAKMTKND
                     ENNYLLLMPSKSNDEGFAMTGSFSCMMLSAMLIFDSLEDDVEKSYINAIIEMGRNVID
                     RKDEIHELINKDFDRVVYLGSGGLGGLTQEAQLKLLELTAGKISTVYDSPMGFRHGPK
                     SFIDENTLVFEFVSNCLYTRKYDLDVLEEIKRDKIAKFTCAVSVENENNFSGTKFEFK
                     EKYNKLPDVYLAMPYILFAQTIALFVSVKVGNKPDTPSATGTVNRVVKGVTIYEY"
     gene            2640..3491
                     /gene="gatY"
                     /locus_tag="PC700_000646"
     CDS             2640..3491
                     /gene="gatY"
                     /locus_tag="PC700_000646"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HDF2579970.1"
                     /translation="MALVTTTSMLKKAQDEGYAVGAFNVENMEMVIAVIEAAEELNSP
                     VILQTTPSTVKYAGLDYYLANAKVAAERVKIPVAMHLDHGSSFDLAMKALRSGYTSIM
                     IDGSHESFENNISISKSVVKACSPSLIPVEAELGKVGGKEDDLDGGNSNDYTNPKEAK
                     EFVDRTGISSLAIAIGTAHGLYKGEPKIDLDRLSEIKNVVSIPLVLHGGSGIHDDIIK
                     EAIKRGIAKVNYATELRIAYSKGVKKVLDEDSEVIDPKKYGLAGLQSVKDFVKEKIKV
                     CGSVNRV"
     gene            3817..5718
                     /locus_tag="PC700_000647"
     CDS             3817..5718
                     /locus_tag="PC700_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2579971.1"
                     /translation="MRERIILETLKNSSGYLNIDYFADKLGVSTRTIRNEIKKIETIK
                     ECNGFKLEYKSKLGYILNIKDQDKFEHYLRNLPSDLVENPEQRLESIIVELLVNEGYK
                     TIEQLSKKFLVSSSQIKNDLKKIDEKIKDTELKLERKAHYGIKIEGSIKEIQSILVDS
                     YFRGNRNITEYRNKFIDNLKLANIRSTIKNVLNEHDLEANLTELEEILAQIIILYIRV
                     SMRVVGNASNLKLYTEDLIIDKLLDKIFRDKKYNLNYDEKYYLKELIKLKTKDKKATI
                     KNIDKNKLQDIMFEFFREIDKKYNSNFLEDKEFFNLFYLHIACLIERIKKNHKIINPF
                     SVKISQQYPTVFNLSIQFSKIIENEYHIKISQDEIGFIATHIAVPFEKREEANFNKKY
                     KIAIICSSGGGSAFLINLRLKEIFPNAEIRNFSLLEEKAVIEFAPDLIFSITNLLFEI
                     NAPVILINEILDELDYLDIKESVRFADHIGNISSPKQYILGLFDKNHFRCIKDKAKYK
                     DILDSMSKCIVDEGACSPTYPKDVWERESYLSTIYTNGVAIPHPIEMTGNKNIISVAL
                     IQTDIIYENRVPKIIFMISLIKGNLELHKQISKYLSKIMANKDMVDMLNKSQSYEEFM
                     YKLKIYLGG"
     gene            5721..6764
                     /gene="ypdE"
                     /locus_tag="PC700_000648"
     CDS             5721..6764
                     /gene="ypdE"
                     /locus_tag="PC700_000648"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDF2579972.1"
                     /translation="MNIEFFKRLSEADGIASNEEEVRHVLLEELKAYSDKIICDGLGS
                     IIFSKIKDESAPNVMICAHMDEVGFMVRSIDKLGMIHLITIGGVKPLAQFVQKVRITT
                     KEGKKIPAVINATYNNGKAENIYADIGAYTEEDVYNLGINVGDMVTYTTSFEEFTLPD
                     RLVGKAFDDRIGCFVMGEVLKELRKENLNCNIHFAATSSEEVGIRGAKTSTQLINPDI
                     VFVIDVACAKNEFVRDYTNQKQIDKGVMLMHRDRTLVPSRKMIDYVMEIADKNNIPLQ
                     HDMFESGGTDGGEAHLVNEGKPCVVTCVPVRYGHCAFSIVSNKDLENIIRLYTQLILN
                     FDEEQYKHIRNFI"
     gene            6779..7084
                     /locus_tag="PC700_000649"
     CDS             6779..7084
                     /locus_tag="PC700_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2579973.1"
                     /translation="MSTEVQIMELISTAGESKSKAFEALKKVKSKDFKGAKLLIEEAR
                     KIDIEAHRIQTELITQDLNEDVESPTMSLLMVHAQDHYMTSQLAKDLIEALIYCLDK"
     gene            7181..7516
                     /locus_tag="PC700_000650"
     CDS             7181..7516
                     /locus_tag="PC700_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2579974.1"
                     /translation="MKILLCCAGGFSTNMLMQNMKKVVKESAKLNEKDFEFTAIPADS
                     IESEVDDWDIVLIGPQIAHKIDFLKGILEPKNKPFAVIDKDVYGKMDGATVMKLALIT
                     YKKHQLAQG"
     gene            7548..8870
                     /locus_tag="PC700_000651"
     CDS             7548..8870
                     /locus_tag="PC700_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2579975.1"
                     /translation="MFSKFEAFMNRVFMPLAHKVDNQRHLGSIKAGMVAMTPFTILGS
                     IFAILPALPNMLGENNPISKFILNNSAILDLPVSLSIGLIGLYACMCIAFNLGNYYKL
                     YIPGCVALSTFSFLFLVASFTPDGALEIGNLGAKGLFTGMIAGLGSVELYNFCNKKNI
                     TIKMPENVPDFVSRSFELIPTTIIVCGTFIVARFISLKVFGELPPQILTQFLSPLVGS
                     MDNPWSVLALNFAICTIFFFGIHSSVFSPITRPIMITFIAENIAAMQAGETIPHFFTA
                     GASSAFFGFTGCGISIGCVIACMLSKNASYKKIGRVSLFPALFGINEPILFGAPIILN
                     PIMFIPHVFGGAIIGTLPMFFMHWGWLAKPVFDPPYVGVFLEGFLTNGDWHSILANAL
                     QLVLAVLLYWPFFKIMERQQNNKKQKEETKNIFSKEEEDLLDDLGLDF"
     gene            8880..9950
                     /locus_tag="PC700_000652"
     CDS             8880..9950
                     /locus_tag="PC700_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2579976.1"
                     /translation="MNNTIERLVSLIYKRGVDALLIKAKNTKRYIGALSGSGVYVLVT
                     KDKNYQILDGRYIDEADKKTTGFIKRIVSQGSYIPTIIELLKELNIKKLAIENQAMSI
                     QEYLSLKNEGFEINLITNELWKLRAIKSKEEIELIKKACEITDEVFDEVISEIKKDMT
                     ELEVSALIQYHALKKGASGMSFETIVVSGERGAMPHGRPTNKKLKVNEAITIDFGVVY
                     QGYQSDMTRTISIGKPPKIIKEIYDVVLEAQLSAIESIKEGTRASDVDKVARKIIDKH
                     GFGEYFNHGLGHGIGLGDGEVPTLNPNSEDILVEGMVMSCEPGIYIPNVGGVRIEDDI
                     VIIDGKGVPLNKTSKEFIILGE"
     gene            9955..11142
                     /locus_tag="PC700_000653"
     CDS             9955..11142
                     /locus_tag="PC700_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2579977.1"
                     /translation="MKYNFNKVLNRKEGNCRKWSNHVIKEKFGLNEDAIPMDLADIDF
                     ECAPAIKESILKRASVGDYSYTFIGDDFYDAIINWNKRRFDVYIEKDWIKLTFGTVST
                     LHYIVQAYTKEGEGVLINTPAYDPFAEAVANNNRKLCCSSLKLDNNRYYLDLEDMENQ
                     MKYENIRLLIFCSPQNPSGRIWTKEELYQVSELCLKYNVILVSDEIHRDIIFKDYRFV
                     SLWNAHPEIYKNSIICVSPNKGFNLGGLKTSYVLIRDVKIRETLLERLKSNSITSPNV
                     FAIPAIVAAYNESEEWLDAMTSYVEENFEIVYDFFETNIPKAKVMKSDSSFLAWIDVR
                     EVFRNEEESKEFFRHANLTMVVGSYFVKDGDGFIRINVGCPRETLNEALNRIKKTYIS
                     MYC"
     gene            complement(11339..12211)
                     /locus_tag="PC700_000654"
     CDS             complement(11339..12211)
                     /locus_tag="PC700_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HDF2579978.1"
                     /translation="MNILQNENLIIESSSFGAELTKIFSKKYDKNILWDGNKKYWGRQ
                     SPILFPIVGKLFDNETIIEENLYSMTQHGFARDMDFEIVDKGDTFVSYKLTDNESTLK
                     KYPYSFELIINYTLNDNSIEVEWIVKNTDSKDIFFSIGGHPAFNLPFYKQNDFSKHYL
                     EFKSKNDVEKINLNGSFTDDIIPIGKLKNLHLNPEVFKNDALIYTNIDEVSIYNNDGS
                     KYVTISMEDFPLVGIWTPYYSETNSTAPFLCIEPWYGLADSINSNKIYKDKKFINKLS
                     KGKVFTASYNINIH"
     gene            complement(12605..12796)
                     /locus_tag="PC700_000655"
     CDS             complement(12605..12796)
                     /locus_tag="PC700_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2579979.1"
                     /translation="MDDFSKLKSLYEDGYRCIYHDCVDNNYTIYLKNFYTEGSETIEL
                     SSESDFSQFKDYIDGLRMS"
     gene            13016..14062
                     /gene="cobT"
                     /locus_tag="PC700_000656"
     CDS             13016..14062
                     /gene="cobT"
                     /locus_tag="PC700_000656"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HDF2579980.1"
                     /translation="MSLLESISKNIYSLDNSSIEKTKQRLDRLIHPTGSLGKIEDICM
                     QLAGIFGNENFDTSKKVIIAFAGDHGVYEEGVAPDPQNITKLQFPNFSKGLCGVGVIS
                     KFVGADVVAVDVGINCDEKLDGVLDYKIRKGTSNMAKGPAMSKQEAIRCLEIGIEIAE
                     QCIEKDYKVIGIGEMGIANTTPSTAIISVISGCDPLEVTGIGAGLKKERLKHKAEVIR
                     KAIEINNPNPTDGVDILSKVGGFEIGSMAGVILGCSANRIPVVIDGFISYAAALIAYK
                     INPKTREYMIASHLSAESGTKRALDILKLDPLLNMDMRLGEGSGAALAFDIIEASNYT
                     YKNMATFDEIDMGR"
     gene            14094..14654
                     /gene="cobU"
                     /locus_tag="PC700_000657"
     CDS             14094..14654
                     /gene="cobU"
                     /locus_tag="PC700_000657"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HDF2579981.1"
                     /translation="MSKIILVTGGARSGKSNFAEKLCFDRNNNTAYIATSIPFDDEMK
                     DRVKKHQENRPRNWNTYEIYEDIYSIIKEIYKEHETVILDCVTLLVNNLMFEHNLNIE
                     ESSQEEINKLEQYIKEQVVKLIEEIEKTNLYFVFVTNELGMGIVPGNKLSRIYADIVG
                     RINQYIASKSNEVYFVVSGIAMKIKE"
     gene            14664..15437
                     /gene="cobS"
                     /locus_tag="PC700_000658"
     CDS             14664..15437
                     /gene="cobS"
                     /locus_tag="PC700_000658"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HDF2579982.1"
                     /translation="MKRFILILQFLTRIPIKLNVGFDDEFYKSIVYFPLVGFVIGILS
                     YLIGWISMLLFEPFIASIIITLAGVLITGGLHIDGLGDTFDAIYSYRDKEKMLEIMKD
                     SRLGTNSLLAIMFVLLLKVGFVYDIISNNSLWIIIFMPMIARLGVMLLTYKTVTPREK
                     GMGNLFIGKLTTSMLITAIIYTLLIVALITKFIFLLPNIVLIKVLGSIIVVFVFIILF
                     KKHIYKKIDGVTGDILGCGIELSELVYLIYIYLLIFMFF"
     gene            15457..16071
                     /locus_tag="PC700_000659"
     CDS             15457..16071
                     /locus_tag="PC700_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDF2579983.1"
                     /translation="MIRLILIRHALTNDNKKGRLSGHINSCISEEGKLQINKITRYLS
                     NENIDKVYTTPSTRTKDTVEKISKLKLLEIEEKEALREISFGDFEGRTFEEIKIKYPN
                     EFEKMIKEGNNYRYPNGESLIDSYKRVAKEIDNIILENDSNLDTKTILICSHAGTIRN
                     IITHLISGSYKYHWNFKIDNASITVLEIDGGFAVIDKMNFTDFI"
     regulatory      16138..16317
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            16447..17946
                     /locus_tag="PC700_000660"
     CDS             16447..17946
                     /locus_tag="PC700_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HDF2579984.1"
                     /translation="MLQGTASNVGKSVLVAGLCRIFKQDGYTVAPFKSQNMALNSFIT
                     KEGLEMGRAQVFQAEAAKIEPIADMNPVLLKPSGNNKSQVIVRGKVVGEMPSSKYHDY
                     KLELVGVLKETFNSLNSRYDIVVMEGAGSNAEINLKDRDISNMGMAEIADAPVIIVGD
                     IDRGGVFASLAGTMLLLNEDEKRRVKGVIINKFRGKKELLQDGVKMLEDIIKVPVIGV
                     VPYTDIKIEDEDSVTTRFKKKMNKSDIHIEIIRTPHMSNFTDFNIFETQEDVSIRYVD
                     YGESLGNPDILIIPGTKSTIDDLTYIRKSGLESQIKNLHSQGKLIFGICGGYQMLGKK
                     LKDPYHVESEIEEVDGIGLLDTETTFEKEKTTTQVEATIVECISEYMNNLKGKKVKGY
                     EIHMGITKRGNDVRSLDLITKKLDKEVNYTEGSVNKEGNVIGTYLHGIFDEISFTRAI
                     LNNIRKKKGLGELESKVNSFNEFKDKEYDKLADFLREHLDIEKIYEIMK"
     gene            17985..19361
                     /locus_tag="PC700_000661"
     CDS             17985..19361
                     /locus_tag="PC700_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HDF2579985.1"
                     /translation="MKKILIAGTNSGVGKTTISLGIMQALVKRNMKVQPYKVGPDYID
                     PSYHTFITGRHSRNLDSYMLDDEKIKYIVNKSSKDADISVIEGVMGLYDGFGIDLDNC
                     TSSHTSKVLKSPVILVINGKSMAASSAAMVLGYKELDKDVNIKGVIVNNVKTNSHYQI
                     IKSSIEKYCNVEVLGYFPPNNKFSLESRHLGLVPSVEMKSLREKFYTLADEIEHYIDI
                     DRIIEISESDEFESDFDFQTLIENNKIKEHVNNKSIAIAYDKAFNFYYRENIELLEEL
                     GLEINYFSPLEDASVPNADYIYIGGGFPEIFGKELEANESMRTSILEAHNNNIPIYAE
                     CGGLMYLGEKLLNQEEQEFNMVGIFSGISKMTSSLKRFGYCIGVAKENTLLSKKGESI
                     KGHEFHHSIFESDEKCVYSMRKERDGNIVEEWDGGYSKGNTLATYLHTHFYNNLNCIE
                     NFLKKGNE"
     gene            19362..20333
                     /locus_tag="PC700_000662"
     CDS             19362..20333
                     /locus_tag="PC700_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HDF2579986.1"
                     /translation="MSILSIYIGYVTDLIVGDPYSFPHPVRFIGKLINFTQGIIRKVS
                     KNDRQLKFGGFILWFITVGITYLTTYAIVKLFSFNILLSIVVNSFIIYTTLATKCLKD
                     EALKIYNVLKTEDIEKSRIQLSYIVGRDTTNLSEPEIIRATVETVAENTVDGIIAPMF
                     YAFIGGAPLAMAYKAINTLDSTVGYKNKKYKDIGFASAKIDDIANYIPARISVILMTI
                     GSFFLNYNYRNCFKISIRDRKNHKSPNCAFSEGAVSGALGIQLGGTNVYFGEKVYKPT
                     IGDKLREIDKEDIIKTNKIMYVSSFVSMLVFTLIHVLCRMILINLII"
     gene            20365..21435
                     /locus_tag="PC700_000663"
     CDS             20365..21435
                     /locus_tag="PC700_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2579987.1"
                     /translation="MKDLGHGANVGEMARLYGKNPKEIIDFSSNINPNVLPNLERYIL
                     KGLEECRNYPDINYTNLRENISKYIGINPDFIIPGNGATEVIYLLMKSVKKKLAIINP
                     TFSEYRRSAELNNLDIIDLELDLENNFKLNIDIIKENIKRFDSLFICNPNNPSGNVQD
                     LKELVHLLGKHNKVLIIDETFMEFVEDESKYSLVKYIESNKNIFIIKAVTKFFGMPGL
                     RLGYGLTSNTEIMNKIYEHKEPWTINSFADILSNFIFEDKEYIKNSKEYYIEERKYML
                     QELRNIRNIKVYDTDANFILIRAYKKTTKELKKDLLKQGNILVRDASNFIGLDDSFIR
                     VAIKSHEDNKILIENMKNLLGD"
     gene            21437..22324
                     /locus_tag="PC700_000664"
     CDS             21437..22324
                     /locus_tag="PC700_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HDF2579988.1"
                     /translation="MKSYGICPASCGEFVQGIIDDEEYLCSYAIDMYSEVYIEEKLVD
                     INLGRYKSRLAIEKVFEKFNLPKKYTKNISLNINSKIPVGKGMASSTADIGATIKATL
                     SLIDKDLSSEEISKLAAEIEPTDSIFIDKNSIFNPLNGTVIKYLGNLTNAKVIILEPN
                     KVLDTMKIRLRQDYNKLKVENKEVIKKSFALLEEGLKKNNLSLVGEACTLSSLANENI
                     EKKEYLNEIIKISKKYGAYGVNIAHSGTVVGILIDKSMNDKKMIDTLCESNINSVYNK
                     IYTQNIINGGIKGEIEWNT"
     gene            22312..22944
                     /locus_tag="PC700_000665"
     CDS             22312..22944
                     /locus_tag="PC700_000665"
                     /EC_number="5.4.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003724727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-8 methylmutase"
                     /protein_id="HDF2579989.1"
                     /translation="MEYIKNPMKIEEKSFELIQEIIDEIRPDYKFKNNIEEKIIKRAI
                     HTTADFDYLDILKISEEAVDSIIDALKNNASIYTDTNMALSGINKRKLESLGCKYKCL
                     VADDETVKLAKEKGITRSMAAVEIAAKEEGRKIFVLGNAPTALYKVMEMKSEGRLDLD
                     AVIGVPVGFVGAQESKDEVEKTDIPYIISKGRKGGSNLAAAIVNAILYTM"
     gene            22953..24179
                     /locus_tag="PC700_000666"
     CDS             22953..24179
                     /locus_tag="PC700_000666"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HDF2579990.1"
                     /translation="MEEYVYIDGKKYRRGYTTGSCATGASKAAVYMLITKNRINTINI
                     DTPKGIPLLLKVDNINISDTFVECSIKKDGGDDIDATHTMDIYARAEIVAKNDKNKGY
                     LTLKDIDSLSTNSECKSELYKFIRVYGGTGIGVVTKKGLSVDVGKPAINPTPLKMINH
                     EIRKLIGDNFESILGNDKVLKITIFAPQGETVAKKTFNPRLGIVGGISIIGTTGIVEP
                     MSDEGWKKSLSIELQMKKEQGLDKIILVPGNHGEQFIREKLNLDIKYVVRVSNFIGYM
                     IKEAQRIGYKKILMAGHIGKFIKVSAGIFNTHSKVADARSEILVANLALMGARYEFLN
                     KINQCVTTEEAVELINNSEYREVYNILSNKCKERVKQYLNEDSDDIDVEVIIFSMDKS
                     LLGKSDNTDDLVEVFI"
     gene            24176..24784
                     /locus_tag="PC700_000667"
     CDS             24176..24784
                     /locus_tag="PC700_000667"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HDF2579991.1"
                     /translation="MINIIGLGPGNLDYITKKGENLISTSDVLIGGKRNLESIKNFEG
                     EKIVLDSNLREIIEYINNNKEKQISIIASGDPLIYGIGRYLSKNIDNKMLNVVSGISS
                     LQYIFSKIYVDMNDVYITSSHGKIPDFDYILSHKKVCMVTDSKIGPKQISREIIDRNL
                     NKIIVVGENLSYDNEKITIAKPEEIIRMDNFDMNVVVILDEE"
     gene            24774..25349
                     /locus_tag="PC700_000668"
     CDS             24774..25349
                     /locus_tag="PC700_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating cobalt-precorrin-6B
                     (C(15))-methyltransferase"
                     /protein_id="HDF2579992.1"
                     /translation="MRNSEFITGKVPITKEEVRAISISKLNLVNAKNLIDIGAGTGSV
                     SVEAAYNYPNLKVISIEKNDDAIELIEKNIKKFNLKNIEVIKGYAPINLGLNVKVESI
                     FLGGTGNNLEEIIEWSKKNLVTGGRLVANFIIIDTFNQTLKLLKKHGFKEIDVCVLNV
                     SKLEKLGKGEYFKPLNPIYIISCEKGENDDE"
     gene            25342..26094
                     /locus_tag="PC700_000669"
     CDS             25342..26094
                     /locus_tag="PC700_000669"
                     /EC_number="2.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4 methyltransferase"
                     /protein_id="HDF2579993.1"
                     /translation="MNKVHFVGAGPGDKELITLKGYKLLSNADVVIYAGSLVNPELLE
                     YCKEDCQIHNSAHMDLQEIIDVMREGIENNKSVVRLQTGDFSIYGSIREQVEDLNKLN
                     IDYDCTPGVSSFLGAASSLGVEYTVPEISQSVIITRMEGRTPVPEKESIQSYAKHQTS
                     MVIFLSVQEIEKVVSKLLEGGYPKDTPIAVIYKATWADEKIVKGTLSDIAVKVKENNI
                     NKTALIMVGRFLGEEYNNSKLYDKDFKHEYRG"
     gene            26078..27199
                     /gene="cbiG"
                     /locus_tag="PC700_000670"
     CDS             26078..27199
                     /gene="cbiG"
                     /locus_tag="PC700_000670"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HDF2579994.1"
                     /translation="MNIEGNIAIICITENGKNLAVKINSLVNNCVIYQVKNKKSNLVV
                     EETCVHTVQKKLSDFVGEIFSKFDYIIFIMATGIVVRSIAPYIISKFSDPAILVMDEK
                     GKNVISLLSGHIGGANSLTQYISSLIDANPVITTATDINQKASLDMIAKKLNAHIDNF
                     RESVKEVNSMLVNNKCVGLYVDEGYQVDTRGFKLIESKDELRDSFSNTYIEKLVVVSN
                     KINCIEDIENLNLDNKFEHCYSTKKIIKVIPRDIVIGIGCRRNTDSELMYNSLEEFLN
                     KQNIDIRAINTVGSIELKKDEKAIIELSKILKVPFKTFSIDEISKVDNLFDKSDFVKK
                     NVGVYSVAEPIAYLLSKGNLLVEKHKYKGITFSVGRIKI"
     gene            27196..27921
                     /gene="cobJ"
                     /locus_tag="PC700_000671"
     CDS             27196..27921
                     /gene="cobJ"
                     /locus_tag="PC700_000671"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011701973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HDF2579995.1"
                     /translation="MIYVVGIGPGSKDTMTLEAIKAIEDSEAIVGYKTYIKLIEEFIQ
                     GKEIIQNGMRQEVDRCKQAVEEAKKGKKVAVVSSGDAGIYGMAGLILELISKEDEDIK
                     VKVIPGVTASIGAAAILGAPIMHDFCHISLSDLMTPWEVIEKRLRLAAEADFVICLYN
                     PRSKGRSEHLSKAFKIMGEFKGGDTPVGVVKDVGREKEEKFVCTFENMDFEKVDMTTM
                     VIIGNKSTYINGELMITPRGYTV"
     gene            27918..28670
                     /gene="cobK"
                     /locus_tag="PC700_000672"
     CDS             27918..28670
                     /gene="cobK"
                     /locus_tag="PC700_000672"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HDF2579996.1"
                     /translation="MILILGGTSDSLSICDKINELKNQPYILSVTTDYGRELAQQHAE
                     NVILGKLGKEDMLKFIKDNNIVKIIDATHPYAVEVSKTAITCAKLLNIDYIRFERKSL
                     IEEIDYDNKFIVDTIEEACEIANKIGTNIFIGTGSKNLNRFIEEISDKNLVVRVLPTS
                     EVILSCEKLGLSADNIVAMKGPFSQYINEETYKHYNIDLVITKESGIAGGFLEKVNAC
                     KTFNIPVIIIKRESINYENVFNDINKIKDILV"
     gene            28689..30167
                     /locus_tag="PC700_000673"
     CDS             28689..30167
                     /locus_tag="PC700_000673"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HDF2579997.1"
                     /translation="MKKALLVVSFGTSYHETREKTIDICEDKIKNSLKSHDFFRAYTS
                     NMIINKIKKRDGIEIDNPIQALDKIYEQGYDEVIIQTLHIICGEEFNKLKEQVDGYSS
                     KFKKIVLGRPLLTHIEDYQEAVEAIKHQIPHMESNEAVVFMGHGTLHESHSAYPALEY
                     MLRDNGINAYVGTVEGYPEIEHVIRRLKEGNIKTVNLMPFMLVAGDHAINDMAGDEED
                     SWKTILEEDGFDVRVHLKGLGENSYIQDKFVRHAVKCAENAKFYGIGAGPGDGSLLTI
                     KAVNTLKKLDILYTPESKKGGDSLALSIVSEYLPESVEIKSRHFPMSFDGNEKSLAWD
                     NVAQEIISDVNDGKNVGFVTLGDPMIYSTYVYIMKRILGEVEVETIPGISSFSNIASN
                     QNFPLVMDKEALVVIPCTMEDDKIEYALQNYNSIVLMKVYKNFKEIIKKLEKYDLIEH
                     AILVSNSSQESENVFTDLKEAHLEDKISYFSTILVNKDNKIK"
     gene            30197..31102
                     /gene="hemC"
                     /locus_tag="PC700_000674"
     CDS             30197..31102
                     /gene="hemC"
                     /locus_tag="PC700_000674"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HDF2579998.1"
                     /translation="MNIVVGTRGSTLALIQTEWVINELKKKYPEISFEIKIIKTKGDL
                     IQNVSLDKIGDKGLFVKEIEQQLLDGKIDIAVHSMKDMPSYLADGLKFAHTPKREDPR
                     DVLILREGYKNLDDLPHGAVIGTGSKRRKFQLLKQRPDLNIVQVRGNVETRIRKIKDE
                     NMHGIVLAASGIIRANLQDKISSYLPVDVVIPAPAQGALAIEIRSNDSTIEGIVNSLK
                     DENTEIQILAERGFLDGVNGSCHIPMAAYCEIIQDKIHLTGLYGDSEGKKVVIKSIDG
                     DISSPRELGLKLAKLVLKEYENYEG"
     gene            31092..32591
                     /gene="cobA"
                     /locus_tag="PC700_000675"
     CDS             31092..32591
                     /gene="cobA"
                     /locus_tag="PC700_000675"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDF2579999.1"
                     /translation="MKGKVYLVGAGPGDYKLMTLKGLECIRKSDVIVYDRLANSSYLR
                     EAKPDCELIYVGKASSNHILPQEDINRIIAEKAKEGKIVTRLKGGDPYVFGRGGEEAE
                     TLVDEDIEFEVVPGITSAIGGLCYAGIPITHRDHASSFHVITGHLKGDDSGELNWNAL
                     ANNKGTLVFLMGISNLEKISENLMKEGKDKDTPVALISWATRYNQRVVTSTLENVYET
                     AIKEEVKPPTLIVVGSVVGLREKLNFFESKPLFGKSIAVTRSRNQNSVLVEKIMDLGG
                     NPIEIPTIKVEKIQNNINLENEIKNINKYNYLILTSKNAVEIFFEKIYEMNFDLRILS
                     NLKVCAIGSATSNELKKRGIIADIVPKKFVAESLYEELAPILNDNDNILIPRAENARD
                     YLVDKLKAICEVTEVHIYRTVIDEDKKGEILDILNSNDIDYITFTSSSTVNNLVEIIG
                     KENIKILEKIKTLSIGPITTETINSLGIKLYKESATSTIDSLVDLIIKN"
     gene            32608..33573
                     /gene="hemB"
                     /locus_tag="PC700_000676"
     CDS             32608..33573
                     /gene="hemB"
                     /locus_tag="PC700_000676"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HDF2580000.1"
                     /translation="MLRRPRRLRASKAIRNLVRETKVNVEDLIYPLFVVEGENIKEEI
                     NSLPDVYHLSIDMLEDEIKEISELGIEHIMLFGVPDEKDECASQAFNDNGIVQKAIRK
                     VKEINSDINVITDVCMCQYTSHGHCGILTDTGYVDNDKSLEYLAKISVSHAKAGADMV
                     APSDMMDGRIQAIREALDENGLENVGIMAYSVKYASNYYGPFREAAHSAPSFGDRKTY
                     QMDPANSNEAIIETELDILEGADILMVKPALSYLDIIRRVKDNFDLPLAAYNVSGEYS
                     MLKFAVKEGLLNEDVILESITSIKRAGADIIITYFAKDIAKLLKK"
     gene            33726..34304
                     /locus_tag="PC700_000677"
     CDS             33726..34304
                     /locus_tag="PC700_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HDF2580001.1"
                     /translation="MLYPINLKLDELDVVIIGGGEVAYRKCKNFLEFNKNVTIVSKQI
                     LNKFYDLKGNIKIIKDDYKEIYIKDASVIIAATNNRELNMEIGLYCRAKNKLVNVVDN
                     IEISNFTVPSYIKRGDLLISVSTGGKSPSLSSKIKKEIEERYTEDYEEYLNVLGDIRK
                     KVIKKYEDKSKRKDVLNMLVTLDLEELKKFDI"
BASE COUNT        13827 a         3496 c         6253 g        10786 t
ORIGIN      
        1 ggttagaaaa attcattttt aacctatttt tataataaat atctatttta ctgaatttat
       61 aattttctct agtaatcttt gagccttgat acactcttct ccattaatag ctggttttat
      121 attattttct atacaatcaa taaaatgatt tgatatatcc tcaaaaccct tttgttttaa
      181 tatatttacc catgcaccac tttgttttaa agttaggttt ccaccttctt ctatttccag
      241 tacattaaga ttcttaactc tttgaatctt accttttgaa agaatttcaa cttgttctaa
      301 ctttgttcca gaatctctat gcatagaggt gtttacagag aaatgtttac ctttaagttt
      361 atgacttaca aatataagat tcttattgtc tgttaaaaat aaatcttctc cgctaatttc
      421 aacatcttca tttgctaacc ataaagcagt gtcaataaca tgtatatagt catctataag
      481 ggtactgtcg aatctaacat ttcttaagga atttaatcca tgtttacata tattgataga
      541 tacgatttct gtagcattat ttttaatttc tttgtacata ggacagaatc tacggttgaa
      601 tccaaccatt aaatttaagt tttttttctt acttaactcg ataagttctt ctccttgatt
      661 tactgttgaa gcaagaggtt tgtctacata tacatgaaca cctaaattta ataatatttt
      721 tataatttcg tagtgtgttt ctgtagagct atgtagaaaa atacaatcac attttttagc
      781 tagggattca atagaatcga atggcataat tctataatca ctacagattt tttccctttt
      841 aactttattt ggagtaaaag ctcctacaaa ctcaaatctc tcagacttag ttaatatagg
      901 taaatatgcc ttttgagcta tggagccaag acctatcatt cccattttta tatttttcat
      961 aatactttct cctaaggttt ttatatttac agaattgatt ataattcaga cacataaatt
     1021 tgtaaacttt gtatcaaaaa ataaatttaa agcttatata agtaaattaa cgaaataaga
     1081 aatattgaca aagtaatttt gtaggatatc tataaaattg attgatataa atattttatg
     1141 tattgacaaa aaattcaaat taatttatat ttggattagt ggtaattacc actttggtct
     1201 gaacaagttt ttatcgtaaa ataaaaatac taaagtatga ttttattaaa tttttaaaga
     1261 taaactataa aattaattaa aatctatagc gtatatttta tagtgagttt aagtagtact
     1321 aaaaaatcta agcaaaaaat tataaaagag tatatttttt tatagtttaa agtggttata
     1381 acctcattac caaatgtaaa cgttacaagt atgaaaggag aatgaaaatg tttaaattag
     1441 aagaacaaaa actaaaagat ttaggtgcaa taataacaac taatgaaata aaacaacaac
     1501 ctgagttatg gttagagact tatgaaatat ataaatctaa caaagaaaaa ttaaatagat
     1561 tcatagatac aataagtaat aatcatgggc agtttagagt tatatttaca ggtgctggaa
     1621 cttcagctta cataggaaat tcaatacttc cttaccttaa gaataaaaat gacataagaa
     1681 aatacatatt tgaagcaata cctactacgg atatagtttc aaacccatat gattacttaa
     1741 aaaaagatat accaacatta ttaatttcat ttgcaagaag tggaaatagt cctgaaagtt
     1801 tggctgcttt aaatttaggg aataagattg tagataattt ttatcattta gctataactt
     1861 gtaatccgca aggagaacta gctaaaatga ctaaaaacga cgaaaataat tacttgttat
     1921 taatgccatc aaaatcaaat gatgaaggct ttgctatgac aggaagtttt tcgtgtatga
     1981 tgttatctgc tatgttaata ttcgatagtt tagaagatga tgtagaaaag tcatatataa
     2041 atgcaataat tgaaatggga agaaatgtta tagatagaaa agatgaaata catgagttaa
     2101 taaataaaga tttcgataga gttgtatatt taggttcagg aggattaggt ggtttgacac
     2161 aagaagcaca actaaaactt ttagaactta cagcaggaaa aatatctacg gtgtatgatt
     2221 ctcctatggg gtttagacac ggaccaaaat catttattga tgaaaataca cttgtatttg
     2281 aatttgtatc taactgttta tatacaagaa aatatgattt agatgtttta gaggagataa
     2341 aaagggataa aattgctaaa ttcacttgtg cagtgtcagt agaaaatgaa aataacttct
     2401 caggaacaaa gtttgagttt aaagaaaagt ataataaatt accagatgtg tatcttgcaa
     2461 tgccatacat attgtttgca caaactatag cattgtttgt atcggtaaag gttggaaata
     2521 agccagatac gccatcagct acaggaacag taaatagagt agtaaaaggt gtaacaatat
     2581 atgaatatta aaagataaga ttaaaatatt ataactaaat aatttgggag ggtatcaatg
     2641 tggcattagt aacaacaaca tcaatgctta aaaaggcaca agatgagggg tatgcagtag
     2701 gagcatttaa tgtagaaaat atggaaatgg taattgctgt tatagaagca gctgaggagt
     2761 taaactctcc agtaatatta caaactactc catctactgt aaaatatgca ggtcttgatt
     2821 attatctagc aaatgcaaag gttgctgcag aaagagtaaa gatacctgta gcaatgcatc
     2881 tggaccatgg ttcaagtttt gaccttgcaa tgaaagcttt aagaagtgga tatacatcaa
     2941 taatgataga tggttctcat gaaagctttg aaaataatat atctatatct aaatcagtag
     3001 taaaagcatg tagtccttct cttataccag tggaagcaga acttgggaaa gttggtggaa
     3061 aagaagatga tttagatggc gggaatagta atgattatac aaatccaaaa gaggcaaaag
     3121 agtttgtcga tagaacagga atatcttcat tggctattgc tataggtact gcacatggat
     3181 tgtataaggg tgaacctaaa atagatttag ataggttatc agaaattaaa aatgtagttt
     3241 caataccttt agtattgcat ggaggttcag gaatacatga tgatattata aaagaagcta
     3301 taaaaagagg tattgcaaaa gttaactatg caactgaatt aagaattgca tatagtaaag
     3361 gggttaaaaa agttttagat gaagattctg aggttataga ccctaaaaag tatggattag
     3421 cagggcttca atcagtgaag gattttgtaa aagaaaagat taaagtgtgt gggtctgtaa
     3481 atagagtata aaaataaaat atcctaaatt tattttagat aagtttagga tatttgttat
     3541 atgaaaaatt tatattaatt atatgtaaat ggtgtatttt tttcatttaa ttgatgaaaa
     3601 atatcagctt ttattggtaa agtattacat atataattaa aatataataa aaattgaact
     3661 atatatttta ttattataaa atatgtatat taaattttgg aatcaaaata tactaggata
     3721 taaaattctt aaataaaaag aaaaggttgt ttttataaaa aatatttttt ataccacttt
     3781 aaaaaacaca ataataacac tgagggaggt ggggatatga gagaacgcat tattcttgaa
     3841 acattaaaaa atagtagtgg ttatctaaac atagattatt ttgcagataa gttgggggta
     3901 tctactcgaa ctatcagaaa tgaaataaaa aagatagaaa ctataaaaga atgtaatggt
     3961 tttaaattag aatataagtc taaattagga tatattctaa atattaaaga ccaagataaa
     4021 ttcgagcatt atctaagaaa cttaccctca gatttagttg aaaatccaga acaacggttg
     4081 gagtctataa tagtagagct tttagtaaat gaaggttata aaactataga acagttaagt
     4141 aaaaaatttc tagtaagttc atcccaaata aaaaatgatt taaaaaagat agatgaaaag
     4201 ataaaggata cagaattaaa actagagcga aaggctcatt atggaattaa aatagaaggt
     4261 tctattaaag aaattcaaag tatattggtg gatagttatt ttagagggaa tagaaatata
     4321 acagagtaca ggaataagtt tatagataat ttaaaattag ctaatattag aagtacaata
     4381 aaaaatgtac ttaatgaaca tgatttagaa gcaaatttaa cagaacttga ggaaatatta
     4441 gcacaaatta ttatattgta tataagagtt agtatgagag tagtgggaaa tgctagcaat
     4501 ttaaaattat atacagaaga tttaattata gataaattat tagataaaat ttttagagat
     4561 aaaaaatata accttaatta tgatgaaaag tattatttaa aggagcttat aaaactaaag
     4621 actaaagata agaaagccac tataaagaat attgataaga ataaacttca agatattatg
     4681 ttcgaatttt ttagagagat agataaaaaa tataactcta attttttaga agataaagag
     4741 ttttttaact tattttactt acatattgca tgtttaatag aacgtataaa gaagaaccac
     4801 aagataataa atcctttttc agttaaaata agtcaacagt atccaactgt ttttaattta
     4861 tctattcaat tttcaaaaat catagaaaat gagtatcata taaaaataag tcaagatgaa
     4921 atagggttta tagctactca cattgctgtt ccttttgaaa agagagaaga agctaatttt
     4981 aataaaaaat ataaaatagc aataatatgc tcttctggtg gaggtagtgc atttttgata
     5041 aatttaagac ttaaagaaat ttttccaaat gctgagataa gaaatttttc attactagaa
     5101 gaaaaagctg taatagaatt tgcaccagat ttgatttttt caataacaaa tcttttattt
     5161 gaaataaatg ctccagttat acttataaat gaaattttag acgaattgga ttatttagat
     5221 attaaagaaa gtgttagatt tgcagaccat ataggaaata tatcaagccc aaaacaatat
     5281 atattaggtc tatttgataa gaatcacttt aggtgtataa aagataaagc taagtataag
     5341 gatatccttg atagtatgtc aaaatgtata gttgacgagg gagcttgttc tccaacctat
     5401 cccaaagatg tatgggaaag agaaagctat ttgagtacta tttatacaaa tggggtggct
     5461 atacctcatc caatcgagat gacaggaaac aaaaatataa tatcagtagc tttgatacaa
     5521 actgatatta tttatgaaaa tagggtgccc aaaattattt tcatgatttc attaataaag
     5581 ggaaatttag aacttcataa gcaaatatct aaatatttaa gtaagattat ggcaaataag
     5641 gatatggtag acatgctaaa taagtcacaa tcttatgaag agtttatgta taagttaaaa
     5701 atctatctgg gaggttagac atgaatatag agttttttaa gaggttgtca gaagcagatg
     5761 gtatagcatc taatgaagaa gaggttagac atgttttatt ggaagaacta aaggcttata
     5821 gtgataaaat tatctgtgat gggctaggga gtatcatctt ttctaaaata aaagatgaga
     5881 gtgcaccaaa cgtaatgatt tgtgctcata tggatgaagt tggatttatg gtgagaagta
     5941 tagacaaact tggtatgatt cacttaataa caataggagg agtaaaacct cttgcacagt
     6001 ttgtacaaaa agttagaatt acaacaaaag aaggaaagaa aatccctgct gtcataaatg
     6061 ctacatataa taatggtaag gctgaaaata tatatgcaga tattggagca tatacagaag
     6121 aagatgtata taacctaggg attaatgttg gggatatggt aacatataca acaagttttg
     6181 aggaatttac tttaccagac cgattagtgg gaaaggcttt tgatgatagg ataggatgtt
     6241 ttgtgatggg agaagtttta aaagaactta ggaaagaaaa tctaaattgt aatattcact
     6301 ttgcggctac aagtagtgaa gaggttggaa taaggggagc aaagacatca acacagttaa
     6361 taaatccaga tatagtattt gtaatagatg tagcatgtgc taaaaatgag tttgtgagag
     6421 actatacaaa tcagaaacaa attgataaag gtgtaatgct aatgcataga gataggactc
     6481 tagtccctag tagaaaaatg atagattatg taatggaaat tgctgataaa aataatatac
     6541 cacttcaaca tgatatgttt gaatcaggtg ggactgatgg aggagaagca catctcgtaa
     6601 atgaagggaa accttgtgta gtaacttgtg taccagttag atatggtcat tgtgcatttt
     6661 caatagtaag taataaagat ttagagaaca ttataagatt gtatacacag ttaattttaa
     6721 attttgatga agaacaatat aaacatataa gaaattttat ctaaaggaga ataagaaaat
     6781 gtcaacagaa gtacaaataa tggagcttat atcaactgca ggagagagta aatcgaaagc
     6841 ttttgaggct ttaaaaaaag taaagtcaaa agattttaaa ggagcaaaat tgttaataga
     6901 ggaggctcgt aaaatagata ttgaggctca cagaatacag acagaattaa taactcaaga
     6961 tttaaatgaa gatgttgaaa gcccaacaat gagtctttta atggtacatg ctcaagacca
     7021 ctatatgact tctcagctag caaaagactt gatagaggct ttgatatatt gtctcgataa
     7081 gtaattgtaa tgtagaaata gattatttat gtaaaaattc ttttaaaagt ttagatgctt
     7141 ttaagagata ataaaaatac aaactttagg aggaattatt atgaaaatat tattatgttg
     7201 tgcaggtgga ttttctacta atatgttaat gcaaaatatg aaaaaggtgg taaaagagag
     7261 tgctaaactc aatgaaaaag attttgagtt tacagctata ccagcagatt caatagaaag
     7321 tgaagttgat gattgggata tagtacttat aggtcctcaa atagctcata aaatagactt
     7381 cttaaaagga atattagagc caaaaaataa gccttttgca gtaatagata aagatgtata
     7441 tggaaaaatg gatggagcta cagttatgaa gttggcatta ataacttata agaaacatca
     7501 attagctcaa ggataataaa aataatgagg gaaaagggag attagatatg ttttcaaaat
     7561 ttgaagcatt tatgaataga gtatttatgc ctttagctca taaggttgac aatcaacgtc
     7621 accttggttc tataaaggct ggtatggttg ctatgacacc attcacaata ttaggaagta
     7681 tatttgcaat acttccagca cttccaaata tgcttggaga aaataatcca ataagcaaat
     7741 tcatacttaa caattcagca atattggatt taccagtaag tctttccata ggtttaatag
     7801 gattatatgc ttgtatgtgt attgctttta atctggggaa ctactataaa ttatatatac
     7861 caggttgtgt tgcactatca actttttctt ttttattcct agtagcctca tttacaccag
     7921 atggagcttt agaaatagga aatttaggag ctaaagggct atttacaggt atgatagcag
     7981 ggcttggttc tgtggagtta tataattttt gtaataagaa gaatatcaca ataaaaatgc
     8041 cagaaaatgt acctgatttt gtatctaggt cttttgaatt aatacctact actataatag
     8101 tttgtggaac ttttatagtt gctcgtttta tttcattaaa agtatttgga gaattacctc
     8161 cacagatact tactcaattt ttatctccat tagtaggaag tatggataac ccatggtcag
     8221 ttcttgcact taactttgct atatgtacta tattcttttt tggaatacat tcatcagtat
     8281 ttagtcctat aactagacct ataatgatta catttatagc tgaaaacata gcagctatgc
     8341 aagctggaga aacaatacct catttcttta cagcaggagc ttcaagtgca ttctttggat
     8401 ttacaggatg tggaattagt ataggttgtg taattgcatg tatgttatca aaaaatgctt
     8461 cctataagaa gattggacgt gtatcattat tccctgcatt atttgggata aatgaaccaa
     8521 tattatttgg agcaccgata atattaaatc caataatgtt tataccacat gtatttggtg
     8581 gagctataat aggtacacta ccaatgtttt ttatgcactg gggatggtta gcaaagccag
     8641 tatttgaccc tccatatgta ggggtattct tagaaggatt tttaactaat ggagattggc
     8701 attctatatt agcaaatgca ttgcaattag tattagcagt tttattatat tggccattct
     8761 ttaaaataat ggaacgtcaa caaaataata aaaaacaaaa agaagaaact aagaatatat
     8821 tctctaaaga agaagaagac ttattggatg atttaggatt ggatttctaa tattttgtta
     8881 tgaataatac aatagagaga ttagtatcct taatctataa aagaggtgtt gacgctcttt
     8941 tgattaaagc aaaaaatact aaaagatata taggagcttt atcaggtagt ggtgtatatg
     9001 tgcttgttac taaggataaa aattatcaaa ttctagatgg cagatatatt gatgaagcag
     9061 ataaaaaaac gactggtttc ataaaaagga ttgtatctca aggttcttat ataccaacta
     9121 taattgagtt attaaaagaa ttaaatataa aaaagttagc aatagaaaac caagctatga
     9181 gtatacaaga gtatctttca ctaaaaaatg aaggttttga aatcaattta ataacaaatg
     9241 aattatggaa gctaagggct ataaaatcta aagaagaaat tgaacttatt aaaaaggctt
     9301 gtgagattac agatgaagta tttgatgagg ttatttctga aattaaaaaa gatatgactg
     9361 agctagaagt atcagctcta attcagtatc atgcattaaa aaaaggtgca agtggaatgt
     9421 cttttgaaac aatagtggta tcaggagaaa gaggtgcaat gccacatgga agacctacaa
     9481 acaaaaaatt gaaagtgaat gaagctataa caatagattt tggtgttgtt tatcaagggt
     9541 atcaatcaga catgactcgt acaatatcta taggtaaacc tcctaaaatt attaaagaga
     9601 tatatgatgt agtattggaa gcacaattat ctgcaataga gtcaataaag gaaggaacta
     9661 gggcaagtga tgttgacaaa gtggctagaa aaattataga taaacatgga tttggcgaat
     9721 attttaatca tggactgggt catggtatag gtcttggaga tggtgaagtt cccactttaa
     9781 atccaaatag tgaagatata ttagtggaag gaatggtaat gtcttgtgag ccaggtattt
     9841 atattccaaa tgtaggggga gttagaatag aagatgatat tgttataata gatggaaaag
     9901 gtgttccact caataagaca tccaaagagt ttataatatt aggagagtaa aattatgaaa
     9961 tataatttta ataaagtatt aaatcgtaaa gaaggaaatt gtagaaaatg gagcaaccat
    10021 gtgattaaag aaaaattcgg cttaaatgaa gatgcaattc ctatggattt agctgatatc
    10081 gattttgagt gtgctcctgc tataaaagaa tctatcttaa aaagagcatc agtaggagat
    10141 tatagttata cttttatagg agatgacttc tatgatgcta ttataaattg gaataaaaga
    10201 agatttgatg tatatataga aaaggattgg ataaaattaa cttttggtac tgtttcaacc
    10261 cttcattata tagttcaagc atatactaaa gaaggagagg gtgtattgat aaatacacct
    10321 gcttatgacc cttttgcaga ggctgtagca aataataata gaaagttgtg ttgtagctca
    10381 ttaaaattag ataacaatag atattattta gatttagagg atatggaaaa tcaaatgaaa
    10441 tacgagaata taagattact tatattctgt agtccacaaa atccatcagg tcgtatatgg
    10501 acaaaagaag aactatatca agtgtcagag ttatgtttaa agtataatgt aattttggta
    10561 agtgatgaaa ttcatagaga tataatattt aaagattata gatttgtatc tttgtggaat
    10621 gctcatccag aaatttataa aaatagtata atctgcgtat ctcctaataa aggcttcaac
    10681 ttaggtggat taaaaacatc atatgtatta ataagagatg taaaaattag ggagacttta
    10741 ttagaaaggc taaaaagtaa ttcaataact tctccaaatg tatttgcaat accagcaata
    10801 gttgcagcat acaatgaaag cgaagaatgg ctagatgcta tgactagtta tgtagaggag
    10861 aattttgaaa tagtttatga tttctttgaa actaatatac caaaagccaa agtgatgaag
    10921 tcagactctt cttttttagc ctggatagat gtaagagaag tatttagaaa tgaagaagaa
    10981 tctaaagaat tttttagaca tgcaaactta actatggtag ttggaagtta ttttgtgaaa
    11041 gatggagatg gatttattcg tattaatgtg gggtgtccta gagaaacatt aaatgaggct
    11101 ctaaatagaa tcaaaaaaac gtatataagt atgtattgtt aaatcataaa aaaagtatct
    11161 atggtttata taaaaacaca ttatttagtt atcaaatatt actggaattt tgtattccag
    11221 taatattttt tgattaagga atattattga ggaatattaa ataatattat ttcataccct
    11281 tatattaact tatataaaaa tattttatta ttttgggaac gggttagcta acctttatct
    11341 agtgtatatt tatattgtaa gaagcagtaa aaacttttcc cttactaagt ttattaataa
    11401 acttcttatc tttataaatt ttgttagaat ttatactatc agcaagacca taccaaggtt
    11461 ctatacataa aaaaggagca gtagaattag tttcactata ataaggagtc caaataccaa
    11521 ctagagggaa atcttccata gatatagtta catatttact accatcatta ttatatatag
    11581 acacttcatc tatgttggtg tatatcaaag catcattctt gaatacttct ggatttaaat
    11641 gtaagttttt taattttcca ataggtatta tatcatctgt aaaagaacca tttaaattga
    11701 ttttttctac atcattttta cttttaaact caaggtagtg tttagaaaaa tcattttgtt
    11761 tgtaaaaagg aaggttgaat gcaggatgtc cacctataga aaagaatata tctttagagt
    11821 cagtattttt tacaatccat tctacctcaa tactattatc atttaatgta tagtttatga
    11881 ttagctcaaa agaatatgga tacttcttta aagtggattc attatctgtt aatttataag
    11941 atacaaatgt atctccttta tctacaatct caaagtccat atctctagca aacccatgtt
    12001 gagtcatgct atataaattt tcttctatta ttgtttcatt atcaaaaagt ttacctacta
    12061 tagggaacaa tataggtgat tgtctacccc aatatttttt attaccatcc caaagaatgt
    12121 ttttgtcgta ttttttagaa aatattttag ttagttcagc accaaaactg cttgattcga
    12181 taattaaatt ttcattttgt aatatattca tttaaaatct cctttattat attttatgag
    12241 tatattatac tataaagtat aaagattatt aaattttatt tgatattata tttgaaaggt
    12301 agattttagt atattttgaa aattttgttt tgaaaatttg ggattattta agaaattttg
    12361 tattataaat agaaggaaat agataaattg tatagaataa aatatattaa gataagttaa
    12421 taatgttaaa tgggaagtga gtttatggaa aaaccaaata caaaagttgt taactttcaa
    12481 gagtataaag acagaaaaaa ccaaagagaa gtaaatagag acgaactttt aaagcttata
    12541 aaaaaaattg tgacttcaaa atagggagtt gttaaactcc ctattttaat gggtttcata
    12601 ataattaaga cattctgagt ccatctatat aatctttgaa ttgtgagaaa tctgattcac
    12661 tacttaattc tatggtttct gaaccttccg tataaaaatt cttaagatag atagtataat
    12721 tattatcaac acaatcatga taaatacatc tatatccatc ttcatacaaa ctttttaact
    12781 tagaaaaatc atccacaata tcatctcctt aaagttcttt taaatatatt tttacaaaaa
    12841 gcatctaatt gtatgcaaat tgacaatgta aaataaagga aatgaacata aaatgtagaa
    12901 taatttttat atgtatgtaa aaataaaaag aaaaagttga aatttttaga aaataagagt
    12961 attttttatc taaaaagaga gtttaaaaaa taaaaaaatg ttgggaggat aaaaaatgag
    13021 tttattagaa agtatatcaa aaaacatata ttccctagat aactcttcta ttgaaaaaac
    13081 aaagcagaga ttggataggc ttatacatcc aactggaagc ttaggaaaga tagaagatat
    13141 ttgcatgcaa ttggcaggaa tatttggcaa tgaaaatttt gacacaagta aaaaggttat
    13201 aattgctttt gcgggagacc atggtgtata tgaagaaggc gtagcacctg accctcaaaa
    13261 tataacaaaa ttacagtttc caaatttttc gaaaggtcta tgtggagttg gagtaataag
    13321 taaatttgta ggggcagatg ttgtagctgt agatgtagga ataaactgtg atgaaaaact
    13381 ggatggggta ttagactaca agataagaaa agggacatca aatatggcaa aaggtccagc
    13441 tatgagtaaa caagaagcca ttagatgttt agagataggt attgaaatag cagagcagtg
    13501 tatagaaaaa gattataagg ttattggtat tggggagatg ggtatagcaa atactactcc
    13561 aagtacagca ataatatctg taatttcagg gtgtgacccg ttagaagtta ctggtatagg
    13621 tgcaggtctt aagaaagaaa gactaaagca taaggctgaa gtaattagaa aggctattga
    13681 aattaataat ccaaatccaa ctgatggagt agatatttta tctaaagtag gaggatttga
    13741 aataggctca atggcaggtg ttatattagg ttgtagtgct aataggattc cagtagtaat
    13801 tgatgggttt atttcttatg cagcagcatt aattgcatat aagataaatc caaaaactag
    13861 agaatatatg atagcatctc atttatcagc agaatcagga actaaaagag cattagacat
    13921 attaaaatta gaccctctat taaatatgga tatgagatta ggtgagggaa gtggagctgc
    13981 tttagcattt gatataatag aagcttcaaa ctatacttac aaaaatatgg caacttttga
    14041 tgaaattgac atgggaagat aaatataaaa atttaatatg taggagtgtt ttgatgagta
    14101 aaattattct agtaacagga ggagctaggt cagggaagag taactttgca gaaaaactat
    14161 gttttgacag aaataataat acagcttata tagcaacctc tattccattt gatgatgaaa
    14221 tgaaagatag agtaaaaaaa catcaagaga atagacctag aaactggaat acttatgaaa
    14281 tatatgaaga tatatattcc ataattaagg agatatataa agaacatgaa actgttatac
    14341 tagattgtgt aacattatta gtaaataatc taatgtttga acacaatcta aatatagaag
    14401 agtctagtca agaagaaata aacaaattag aacaatacat aaaagaacaa gttgtcaaac
    14461 ttattgaaga aatagaaaaa acaaatctat actttgtatt tgtaacgaat gagttaggta
    14521 tgggaattgt tcctgggaat aaacttagta gaatttatgc tgatattgta ggtcgtataa
    14581 atcaatatat agcttcgaaa agtaatgaag tttattttgt agttagtggc atagctatga
    14641 agataaagga gtagtatgca attatgaaaa gatttatttt aatactacag tttttgacaa
    14701 ggatacctat aaaattaaat gtaggatttg atgatgaatt ttataagtca atagtatatt
    14761 ttccacttgt aggttttgtc ataggtatat tatcctattt aataggatgg atttctatgc
    14821 ttttatttga accatttata gcttctataa taataactct agctggtgtc ttaattactg
    14881 gaggacttca tatagatgga ttaggagata catttgatgc aatatatagt tatagggata
    14941 aagaaaaaat gcttgaaata atgaaagatt caagattagg tacaaattca cttctagcaa
    15001 ttatgtttgt acttttatta aaggttggat tcgtttacga tataataagt aataattcat
    15061 tgtggattat aatttttatg ccaatgattg caaggttagg agtaatgtta ttgacatata
    15121 aaaccgtgac accaagagaa aaaggaatgg gaaatttatt tataggaaaa ttaactacaa
    15181 gtatgttaat aactgctata atatacacat tattgatagt tgctcttatt actaaattta
    15241 tatttttatt acctaatata gtgttgatta aagtattagg ttcgattata gtggtttttg
    15301 tatttataat attattcaaa aaacatattt ataaaaaaat tgacggagta acaggagata
    15361 tattaggctg tggaatagaa ctttcagagc ttgtatattt aatttacata tatttgttaa
    15421 tttttatgtt tttttaaagt aaaaagtggt gataatatga taagattaat attaataaga
    15481 catgcactga caaatgataa taaaaaagga agattatcag gccatataaa tagttgtatt
    15541 agtgaagagg gcaaactaca aataaataaa attaccagat atctgagtaa tgaaaacata
    15601 gataaggttt atacaacacc atctacacgt actaaagata cagtagaaaa aatatcaaaa
    15661 ttgaaattat tagaaataga agaaaaagaa gctttaagag aaattagttt tggagacttt
    15721 gaaggaagaa cttttgaaga aattaaaatc aaatatccaa atgagtttga aaaaatgata
    15781 aaagaaggta acaactatag atatccaaat ggagaaagtt tgatagactc ttataaaaga
    15841 gtggcaaaag aaattgataa tataatttta gaaaatgata gtaatttaga tacaaagaca
    15901 atacttatat gttctcatgc aggaacaata agaaatataa taacacattt gatttctggt
    15961 agttataagt atcactggaa ctttaaaatt gataatgcat ctattacagt tttagaaatt
    16021 gatggtggat ttgctgtaat agataaaatg aattttacag acttcatata atttaatcaa
    16081 aaccaaacat tacaatctgt gtttgtaata tatctgtaaa attagcaatg aaaatagaat
    16141 attagattaa aattaggttc ttaattagat gttaaaaggg aaaaaggtta gatgcctttg
    16201 cagcccccgc tactgtgaaa ccaacgaaac tataaatacc actgtcagtt tgatgggaag
    16261 gttatagaag taggatgaag ttaagccagg atacctgcct aatttaattt aaaacaagag
    16321 gtcttcgggg tgaaagattt ttaattaata tatataaaag tcctatcttt agaagtatta
    16381 ggactttttt taatttgttt tttagtaaat tttaacaagg agggatggaa agtggggaaa
    16441 aaaataatgc tacaaggaac agcttcaaat gtaggtaaaa gtgtcttagt agcaggtttg
    16501 tgtagaatat ttaagcaaga tggttatact gttgctcctt ttaagtcaca aaatatggct
    16561 ttaaattctt ttataactaa agaaggatta gaaatgggaa gagcacaagt atttcaagca
    16621 gaggctgcga aaatagagcc aatagctgat atgaatccag ttttattaaa accttctggg
    16681 aataataaaa gtcaagttat tgtaagaggt aaagttgtag gagaaatgcc ttcttctaag
    16741 tatcatgatt ataaattgga gttagtaggt gtgttgaagg aaacatttaa tagcttaaat
    16801 tcaaggtatg atattgtagt tatggaaggg gcaggaagta atgcagagat aaacttaaaa
    16861 gatagagata tctctaatat gggtatggca gaaatagctg atgcacctgt aataatagtc
    16921 ggagatattg atagaggagg cgtatttgcc tcattagcag gaacaatgct tttattaaat
    16981 gaagatgaga aaagaagagt aaaaggtgta ataatcaata aattcagagg aaaaaaagag
    17041 cttttacaag atggtgtaaa gatgctagaa gacatcataa aagtacctgt tataggagta
    17101 gtaccttata cagatataaa aatagaagat gaagacagtg taactacgag atttaagaaa
    17161 aaaatgaata aaagtgatat acacatagaa ataattagaa ccccacatat gtcaaatttt
    17221 acagatttta atatatttga gacacaagaa gatgtaagta ttagatatgt agactatgga
    17281 gaatctttag gaaatccaga tatattaata atacctggaa ctaaaagtac tatagacgat
    17341 ttaacatata taagaaaaag tggtcttgaa tcgcaaatca aaaatttaca tagtcaaggt
    17401 aaattgatat ttgggatatg tggtggatac caaatgttag gtaaaaaatt aaaagaccct
    17461 tatcatgttg aaagtgaaat tgaagaagtt gatggaatag gtcttttaga tacagaaaca
    17521 acgtttgaga aagaaaagac tacaacacaa gtagaggcta ctattgttga atgtatcagt
    17581 gaatacatga ataaccttaa gggaaaaaaa gtaaaaggtt atgaaattca tatgggcatt
    17641 acaaagcgag gtaatgatgt aagaagttta gatttaataa ctaaaaaact tgataaagaa
    17701 gtgaattata ctgaaggaag tgtaaataaa gaggggaatg taattgggac ttatcttcat
    17761 ggaatatttg atgaaataag ctttacaaga gcaattttaa acaatataag aaagaaaaaa
    17821 ggtttaggag aattagaaag caaagtaaac tcttttaatg aatttaagga taaagagtat
    17881 gataagttgg ctgatttttt aagagaacat ttagatatag agaaaatata tgagattatg
    17941 aaataatgat gacctttatt tataatttag gtaggaggtg tattatgaaa aaaattttaa
    18001 ttgctggtac taatagtgga gtaggtaaaa caacaatatc attaggaata atgcaagctt
    18061 tggttaagag aaatatgaaa gtacagccat ataaagtagg gccagactat atagaccctt
    18121 catatcacac ttttataact ggaagacatt ctagaaattt agattcttat atgttagatg
    18181 atgaaaaaat aaagtatata gtaaataagt cctcaaaaga tgcagatatt tcagtaatag
    18241 aaggtgttat gggcttatat gatggttttg gaatagattt agataattgt acaagttcac
    18301 atacttcaaa ggtattaaag tcacctgtta tattagttat aaatggtaaa tctatggcag
    18361 catctagtgc tgctatggta cttggatata aggaattaga taaagatgta aatataaagg
    18421 gtgttatagt aaataatgta aagactaata gccattatca aataattaag agttctatag
    18481 aaaaatattg taatgttgaa gtattggggt attttcctcc aaataacaaa ttctctttag
    18541 aatctcgaca tctaggatta gttcctagtg tagaaatgaa atcactaagg gaaaaatttt
    18601 ataccttagc agatgaaatt gaacactata tagatattga cagaataatt gaaatatcag
    18661 aaagcgatga atttgagagt gattttgatt ttcaaacttt gatagaaaat aataaaataa
    18721 aagaacatgt taataataaa tcaatagcta tagcatatga taaagcattc aatttttatt
    18781 atagagaaaa tatagagctt ttagaggaac ttggtttaga aataaactat tttagccctc
    18841 ttgaagatgc atcagtgcca aatgcagatt atatttatat tggaggaggt tttccagaaa
    18901 tatttggaaa agaattagaa gcaaatgagt caatgagaac ttccatatta gaagcacata
    18961 acaataatat acctatttat gcagaatgtg gaggactgat gtacctaggt gagaaactat
    19021 tgaatcaaga agaacaagaa tttaatatgg ttggaatatt tagtggaatt agtaagatga
    19081 catcttcttt aaagagattt gggtactgta ttggtgtagc caaggaaaat actcttttat
    19141 ctaaaaaagg ggaatctatt aaagggcatg agtttcatca ttcaatattt gaaagtgatg
    19201 aaaaatgtgt atattcaatg agaaaagaaa gagatggaaa tatagtagaa gagtgggatg
    19261 gtggttatag taaaggaaac actttggcta catatcttca tactcatttt tataataact
    19321 taaattgtat agaaaatttt ttgaaaaaag gaaatgaata aatgagtatt ttatctattt
    19381 atataggata tgtgacagac ttaattgtag gagacccata ttcttttcct catccagtga
    19441 gatttattgg taaacttata aattttactc aaggtataat aagaaaggtc tctaaaaatg
    19501 ataggcagtt aaaatttggg ggatttatat tatggtttat aaccgttgga ataacatatt
    19561 taacaactta tgctatagtg aagctattta gttttaatat attattgagt attgtagtga
    19621 atagttttat aatttataca acactagcca caaaatgttt aaaagacgaa gcattaaaaa
    19681 tttataatgt attaaaaacc gaagatatag aaaaatctag gattcagcta tcttatatag
    19741 taggtaggga tactactaat ttaagtgaac ctgaaataat aagagcaact gtagaaactg
    19801 ttgcagaaaa tactgtagat ggtataattg cgcctatgtt ttatgcattt attggaggag
    19861 caccacttgc tatggcctat aaagctataa atacattaga ctcaacagtt ggatataaaa
    19921 ataaaaagta taaagatatt ggttttgcat ctgctaagat tgatgatata gccaattata
    19981 taccagctag aatatctgtt attcttatga caattggaag tttcttttta aattataact
    20041 ataggaactg ttttaaaatc tctattagag atagaaagaa tcataaaagt ccaaattgtg
    20101 ctttttcaga aggcgcagta tctggtgctc taggaataca actaggaggt accaacgttt
    20161 attttgggga aaaagtatat aagccaacaa taggggataa gcttagggag atagataaag
    20221 aagatataat taaaactaat aaaattatgt atgtatcttc atttgtatct atgttggtat
    20281 ttacactaat acatgtgtta tgtaggatga ttttaattaa cttaattata taaattattg
    20341 tttgctaact taagggggac atagatgaag gatttagggc atggtgccaa tgtaggtgaa
    20401 atggcaagat tatatgggaa aaacccaaaa gaaataattg attttagttc aaacataaat
    20461 ccaaatgtat taccaaattt agagagatac attttaaaag gattagaaga gtgtaggaat
    20521 tatccagata taaattatac aaacttgaga gaaaatatat caaaatatat aggtataaat
    20581 ccagacttta taatacctgg aaatggtgca acagaagtga tttatttgct tatgaagagt
    20641 gttaaaaaaa aattggctat aataaatccg actttttcag agtatagaag aagtgctgag
    20701 ttaaataatt tagatattat agatttagag ctagatttag aaaataactt taaacttaat
    20761 atagatataa taaaagaaaa tataaaaaga tttgatagtt tatttatatg taatcctaat
    20821 aatcctagtg gtaatgtcca agatttaaag gaacttgttc atttactagg taaacataat
    20881 aaggtactaa taattgatga aactttcatg gaatttgttg aagatgaaag taaatacagt
    20941 ttggttaaat atatagaatc aaataaaaat atatttataa ttaaagctgt gactaagttt
    21001 tttggtatgc ctggtttaag gctaggatat ggtcttacaa gcaacactga aattatgaac
    21061 aaaatttatg aacacaaaga gccatggact attaattctt ttgcagatat actatccaat
    21121 tttatttttg aagataaaga gtatattaaa aatagtaaag aatattacat agaagaaaga
    21181 aaatatatgt tacaagagtt gagaaacata agaaatataa aggtctatga tacagatgct
    21241 aattttattt tgataagagc atataaaaaa acaactaaag aattgaaaaa agatttgctt
    21301 aagcaaggaa acatattagt tagagatgcg tctaatttta taggattaga tgatagtttt
    21361 ataagagtgg ctataaaatc tcatgaagat aacaaaattc ttatagaaaa tatgaaaaat
    21421 ttattgggtg attaaaatga aatcttatgg aatatgccca gcatcttgtg gagaatttgt
    21481 acaaggtata attgatgatg aagaatattt atgttcatat gcaatagata tgtattctga
    21541 agtgtatatt gaagaaaaat tagtagatat aaatttaggg agatataagt caaggttagc
    21601 aattgaaaag gtatttgaaa aatttaattt acctaaaaaa tatactaaaa atatttcttt
    21661 aaatataaat agtaaaatac ctgttggaaa aggaatggca agttcaactg ctgatatagg
    21721 agctacaata aaggcaacat tatcattgat agataaagat ttaagtagtg aggagatttc
    21781 taaattagca gcagaaatag aaccaacaga ttctatattt atagataaaa atagtatatt
    21841 taatcctcta aatggaactg ttataaaata cctaggaaat ctgaccaatg cgaaagttat
    21901 aatacttgaa ccaaacaaag tactagatac tatgaaaatt agattgaggc aagattataa
    21961 taaattaaaa gtagaaaata aagaagtaat aaaaaaatca tttgctcttt tagaagaagg
    22021 attgaaaaaa aataatttat ctctagttgg agaggcttgt actttgagta gcttagcaaa
    22081 tgagaatata gaaaaaaaag agtatttaaa tgaaataata aaaatatcaa aaaaatatgg
    22141 agcatatgga gtaaatattg ctcacagtgg aactgtagta ggtattttaa ttgataagtc
    22201 tatgaatgat aaaaagatga tagatacctt atgtgaatct aacataaatt ctgtctataa
    22261 taaaatttat acacaaaaca taattaatgg aggaattaag ggggaaatag aatggaatac
    22321 ataaagaatc ctatgaaaat agaagaaaaa agttttgaat tgatacaaga aataatagac
    22381 gaaattagac cagattataa atttaagaat aatattgaag aaaaaataat aaaaagagct
    22441 atacatacta ctgctgattt tgattactta gatatattaa aaatatcaga agaagcagtt
    22501 gatagtataa tagatgcatt aaaaaataat gcaagtattt atactgatac aaatatggct
    22561 cttagtggta taaataaaag aaagttagaa tcattaggat gtaagtataa atgtttagtg
    22621 gcagatgatg aaactgtaaa attagctaaa gaaaaaggaa ttactcgttc tatggcagca
    22681 gtagaaattg cagctaaaga agaaggaaga aaaatattcg ttttaggtaa tgctccaaca
    22741 gctttatata aagttatgga gatgaaatca gaaggtagat tagatttaga tgcagttata
    22801 ggagttcctg taggttttgt aggagcacaa gaatcaaaag atgaagttga aaaaacagat
    22861 ataccatata taatttctaa aggtagaaaa ggtggcagta atttagcagc agcaatagta
    22921 aatgctatat tatatactat gtaggtacct atatggaaga atatgtatat atagatggaa
    22981 aaaaatatag aagaggatat actacaggtt catgtgcaac aggtgcatct aaggctgctg
    23041 tatatatgtt gattacaaaa aatagaatca atactataaa tatagatact cctaagggga
    23101 taccattact actaaaagtt gataatataa atatttctga tacatttgtg gaatgttcca
    23161 taaaaaaaga tggtggagat gatatagatg caactcatac catggatatt tatgcaagag
    23221 ctgaaattgt agctaaaaat gataagaaca aagggtatct tactctaaag gatatagata
    23281 gtttaagcac caatagtgag tgtaaaagtg agctctataa gtttattagg gtttatggcg
    23341 gaactggtat tggtgtggtt acgaaaaaag ggcttagtgt ggatgttggt aaacctgcta
    23401 taaatccgac tccacttaaa atgataaacc atgaaataag aaagcttata ggagataatt
    23461 ttgaatctat acttggaaat gataaagttt taaaaataac aatctttgct ccacaaggag
    23521 aaactgtagc aaaaaaaaca tttaatccaa gattaggtat agttggagga atatctataa
    23581 taggtacaac tgggattgtt gaacctatga gtgatgaggg ttggaagaag tctctttcta
    23641 tagaacttca gatgaaaaaa gaacaaggct tagataaaat aatattggtt ccaggaaatc
    23701 atggagaaca gtttataaga gaaaaattaa acttagatat taagtatgta gttagggtaa
    23761 gtaattttat aggctatatg attaaagaag ctcaaagaat aggatataaa aaaatcttaa
    23821 tggcaggtca tataggtaaa tttataaaag tatctgctgg tatttttaat actcatagta
    23881 aggtagcaga tgctagaagt gagatattag tggcaaactt agctttaatg ggagctagat
    23941 acgaattttt aaataaaata aatcaatgcg taactactga agaagctgta gagctaataa
    24001 ataatagtga atatagagaa gtatataata tattaagtaa taaatgtaag gagagagtaa
    24061 agcaatactt gaatgaagat tcagatgata ttgatgtgga agtaatcata ttttcaatgg
    24121 ataagtcttt attaggtaaa tcggataata cagatgattt agtggaggtc tttatatgat
    24181 aaacataata ggtctaggac ctggtaactt agactatata acaaaaaaag gtgaaaattt
    24241 aatttccact tctgatgtat taattggtgg taaaagaaat ctagagtcta taaaaaattt
    24301 tgaaggagaa aaaatagttc tggattctaa tttaagagaa attatagagt acataaataa
    24361 caataaagaa aagcaaatat caataatagc atctggggac ccattaatat atgggatagg
    24421 tagatatcta tctaaaaata ttgataataa gatgttaaac gtggtttctg ggattagttc
    24481 attacagtac atattttcaa aaatatatgt agatatgaat gatgtatata ttacaagtag
    24541 tcatggcaaa ataccagatt ttgattacat attatcacat aaaaaagtat gtatggttac
    24601 agattcaaag ataggaccaa aacaaataag tagagaaata atagatagaa atctaaataa
    24661 aattattgtt gtaggagaaa atctgtcata tgacaatgaa aaaataacaa tagcgaaacc
    24721 agaagagata ataagaatgg acaattttga tatgaatgtt gtggtgattt tagatgagga
    24781 atagtgaatt tataacaggt aaagttccaa ttactaagga agaggttaga gcaatatcaa
    24841 taagtaagtt aaatttagta aatgctaaaa acctcataga cataggggct ggtacaggaa
    24901 gtgtaagtgt tgaagctgca tataattatc ctaatttaaa ggttatatct atagagaaaa
    24961 atgatgacgc tatagagctt atagagaaaa atataaaaaa attcaactta aaaaatatag
    25021 aagtgataaa aggatatgct ccaattaatc tagggttaaa tgttaaagtt gagtctattt
    25081 ttcttggggg aacaggaaat aatctggaag aaataataga atggtctaaa aaaaatctcg
    25141 ttacaggagg aagacttgta gctaatttta taataataga tacatttaat caaactttaa
    25201 aactgcttaa aaagcatgga tttaaagaaa tagatgtatg tgttttgaat gtttctaaac
    25261 tagagaaact aggaaaaggt gaatatttta aaccactaaa tcctatatat ataatatctt
    25321 gtgaaaaagg ggaaaatgat gatgaataaa gtacattttg taggagcagg acctggagat
    25381 aaagagctga taacattaaa aggatacaaa ttattaagta atgcagatgt agtgatatat
    25441 gcaggttcac ttgttaatcc tgaacttttg gaatactgta aagaagattg ccaaatacat
    25501 aatagtgcac atatggattt acaagagata atagatgtta tgagagaagg tatagaaaat
    25561 aataaatctg ttgtaagact gcaaacaggt gatttttcta tatatggttc aattagagaa
    25621 caagtggaag atttaaataa attgaatata gattatgact gtactcctgg agtaagttct
    25681 tttttagggg cagcatcatc tttaggggtt gaatatacag ttccagaaat ttctcaaagt
    25741 gtaattataa caagaatgga aggcagaact ccggttccag agaaagaatc aatacaatca
    25801 tatgctaagc atcagacatc tatggtaata tttttatctg ttcaagaaat tgagaaagtt
    25861 gtgtctaaat tactggaagg tggatatcca aaagatactc ctatagcagt tatatacaaa
    25921 gcaacttggg cagatgaaaa aatagttaaa ggaactttaa gtgatatagc tgtaaaagtt
    25981 aaagaaaaca acataaataa gactgcttta ataatggttg ggagattttt aggagaagag
    26041 tataataatt caaagttata tgataaggat ttcaaacatg aatatagagg gtaatattgc
    26101 tataatctgt attacagaaa atggaaaaaa tttagctgta aagataaatt ctctagtaaa
    26161 taactgtgtt atatatcaag taaaaaataa aaaaagtaat ttagttgttg aagaaacatg
    26221 tgtacataca gttcaaaaga agttaagtga ttttgtagga gaaatattta gcaaatttga
    26281 ttacataata tttataatgg ctacaggtat agttgtacga agtatagcac catacataat
    26341 aagtaagttt agtgaccctg caattttggt aatggatgaa aaaggcaaaa acgtaataag
    26401 tttattgagt ggtcatattg gaggggcaaa cagcttgaca cagtacataa gtagtcttat
    26461 tgatgctaat ccagtaataa caacagctac tgatataaat caaaaagctt ccttagatat
    26521 gatagcaaaa aaactaaatg cacatattga taattttaga gaaagtgtaa aagaagtaaa
    26581 ttctatgcta gtgaataata aatgtgttgg attatatgtt gatgaaggat atcaagttga
    26641 tactagggga tttaaactta tagaaagtaa ggatgagtta agagatagct tttcaaacac
    26701 atatatagaa aaattagttg tagtaagcaa taaaataaat tgtattgaag atattgagaa
    26761 tttaaactta gataataaat ttgaacattg ctatagtact aaaaaaataa taaaggtaat
    26821 tccaagagat atagtgatag gaattggctg tagaagaaac acagatagtg agctgatgta
    26881 taattcactt gaagagtttc ttaataaaca aaatatagat attagagcaa taaacacagt
    26941 tggaagtata gaactgaaaa aagacgaaaa agcaataatt gaattatcaa aaatactaaa
    27001 agtgccattt aaaacatttt caatagatga aatatctaag gttgataatc tttttgataa
    27061 atctgatttt gttaaaaaaa atgtgggcgt gtattcagta gcagaaccta tagcatatct
    27121 tttgagcaaa ggtaatttgt tagttgaaaa acataaatat aaaggaataa ctttttcagt
    27181 ggggagaata aaaatatgat atacgttgta ggaattggac caggaagtaa agatacaatg
    27241 actttagaag caataaaagc aatagaagat tctgaggcta ttgttggata taagacttat
    27301 ataaaactaa tagaagaatt tatacaaggt aaagaaataa tccaaaatgg tatgaggcaa
    27361 gaagttgata gatgtaaaca agcagtagag gaagcgaaaa aaggaaaaaa agtagcagtt
    27421 gtaagcagtg gtgatgctgg aatatatggg atggcaggtc tcattttaga attaatatct
    27481 aaagaagatg aagatataaa agtaaaggta attccaggtg ttactgcaag tattggtgca
    27541 gcagctatac ttggagctcc tattatgcat gatttctgtc atataagttt gagtgattta
    27601 atgactccgt gggaagtaat tgaaaaaaga ttgagattgg ctgctgaagc cgattttgta
    27661 atttgtttgt ataatccaag aagtaaggga agaagtgaac atctaagcaa agcatttaaa
    27721 ataatgggtg agtttaaagg tggagataca cctgttggag tggtaaaaga tgtaggtaga
    27781 gaaaaagaag aaaagtttgt atgtactttt gaaaatatgg actttgaaaa agtcgatatg
    27841 actacaatgg ttataatagg aaataagtct acctatatta atggtgaatt gatgataact
    27901 ccaagaggtt atacagtatg attttaattt taggaggaac ttcagatagt ttatctatat
    27961 gtgataagat aaatgaactg aaaaatcaac catatatcct ttcagttact acagattatg
    28021 gaagggaact tgcacaacaa catgcagaga atgtaatttt aggtaagctt ggaaaagaag
    28081 atatgctaaa atttataaaa gataataata tagtaaaaat aatagatgcc actcatcctt
    28141 atgcagttga agtatccaaa actgctatta cttgtgcaaa attattgaat atagattata
    28201 taaggtttga gagaaaatct ttaattgaag aaatagatta tgataataaa tttatagtag
    28261 atacaattga agaagcctgt gaaatagcaa ataaaatagg gacaaatata ttcataggaa
    28321 caggaagtaa aaatttgaac agatttatag aggaaataag tgataaaaat ttagttgtta
    28381 gagttcttcc aacgagtgaa gtcatattaa gttgtgaaaa attaggttta agtgcagata
    28441 atatagtagc tatgaaaggt ccatttagcc agtatataaa tgaagaaaca tataaacatt
    28501 acaatataga tttagtcata acaaaagaaa gtggaatagc tggtgggttc ttagagaagg
    28561 taaatgcatg taaaactttc aatataccag taataattat aaaaagagaa tcgattaact
    28621 acgaaaatgt atttaatgac ataaataaaa ttaaagacat acttgtgtaa tttttaggag
    28681 gagaatatat gaagaaagcc ttattagtag tgagttttgg tacaagctat catgaaacga
    28741 gagaaaagac aatagatata tgtgaagata aaataaaaaa ttcgttaaaa agccatgatt
    28801 tttttagagc ttatacatca aatatgataa taaataaaat aaaaaaaagg gatggaatag
    28861 aaatagataa tccaatacag gctttagata aaatatatga acaaggatat gatgaagtaa
    28921 taatccaaac acttcatatt atatgtggcg aagaatttaa caaattaaaa gaacaagtag
    28981 atggatattc ttctaaattc aaaaagattg tattaggaag acctttgctt acacatattg
    29041 aagattatca ggaagctgta gaggcaatta agcatcaaat tcctcatatg gaaagtaatg
    29101 aagcagtagt atttatggga catggaacac ttcatgaatc acattcagcg tatccagcat
    29161 tggaatacat gcttagggat aatggaataa atgcttatgt tggtacagtt gaagggtatc
    29221 cagaaataga acatgtaata agaagattaa aagaaggaaa tataaaaact gtaaatttga
    29281 tgccatttat gttagttgca ggagaccatg ctataaatga tatggcagga gatgaagaag
    29341 attcttggaa aacaatatta gaagaagatg gatttgacgt aagagttcat ttaaaaggac
    29401 taggagaaaa ttcttatata caagataaat ttgttcgtca tgctgtaaaa tgtgcagaga
    29461 atgctaagtt ctatggtata ggagcagggc caggagatgg ttctttactt acgataaaag
    29521 cggttaatac tttaaagaaa ctggatattc tatacactcc agaatcaaaa aagggtggag
    29581 atagtctagc tttatcaata gttagtgaat atttacctga atcagtggaa ataaagtcta
    29641 ggcatttccc aatgagtttt gatgggaatg aaaaaagttt agcttgggac aatgtagcac
    29701 aagaaattat atcagatgtt aatgatggta aaaatgtagg atttgttaca ttaggagacc
    29761 ctatgattta tagtacatat gtatatataa tgaaaagaat attaggagag gtagaagtcg
    29821 aaactatacc aggaatttca tcattttcca atatagcatc taatcagaat tttccattag
    29881 tcatggataa agaagcttta gtggtaattc cttgtactat ggaagatgat aagatagagt
    29941 atgctttgca aaattataat tcaatagtat tgatgaaggt atataaaaat tttaaagaaa
    30001 taataaaaaa attagaaaag tatgatttaa tagaacatgc tattttagta agtaattctt
    30061 cccaagaaag cgaaaatgtg tttacagatt taaaagaagc gcaccttgaa gataagatat
    30121 cttatttttc tactatacta gtaaataaag ataataaaat aaaataggtg aaagcaatat
    30181 ttaaaggaga cttaatatga atatagtggt tggaactagg ggtagtactc tagcgcttat
    30241 acaaacagag tgggtaataa atgaattaaa aaagaagtat ccagaaatta gttttgagat
    30301 taaaatcata aagacaaaag gagatttaat tcaaaatgtt tcactggata aaataggtga
    30361 taaaggattg tttgtcaaag aaatagagca acaattatta gatggaaaaa tagacatagc
    30421 agtacatagt atgaaagata tgccttctta tttagcagat ggactaaaat ttgctcatac
    30481 gcctaagaga gaagacccaa gagatgttct aatacttaga gaaggatata aaaatttaga
    30541 tgatttgcct catggagcag taataggaac aggaagtaaa agaaggaagt ttcaattatt
    30601 gaaacaaaga cctgatttaa atatagtaca agttagaggt aatgtggaaa ctaggatacg
    30661 aaaaataaaa gatgaaaaca tgcatggtat tgtgttggca gcatcaggaa taattagagc
    30721 taatttgcaa gataaaataa gttcttattt acctgttgat gttgttattc cagcacctgc
    30781 ccaaggagct ttagctatag aaattagaag caatgatagt acgatagagg gaatagtaaa
    30841 ttcactgaag gatgagaata cagaaatcca aatattggct gaaagaggtt ttttagatgg
    30901 tgtaaatgga agttgtcata ttccaatggc agcttattgt gaaataatcc aagataaaat
    30961 acatcttact ggcttatatg gagattcaga aggaaaaaaa gtagtaatca agtctataga
    31021 tggagatatt tcttctccta gagagttagg tttaaagtta gctaaacttg tattgaagga
    31081 gtatgagaat tatgaagggt aaggtttact tagtaggggc aggaccaggt gattataaac
    31141 tcatgacttt aaaaggttta gagtgtataa gaaaatcgga tgtaatagta tatgatagat
    31201 tagcaaatag tagttattta agagaagcta agcctgattg tgaacttata tatgtaggca
    31261 aggcatcaag taatcatatt ttgccacaag aagatataaa tagaattata gcagaaaaag
    31321 ctaaagaagg aaaaattgta actcgtctta aaggaggaga cccttatgta tttggcagag
    31381 ggggagaaga agcagagact ttagtagatg aagatataga atttgaagta gttccaggta
    31441 taacttctgc tataggaggt ttatgttatg cgggtattcc tataacacat agagaccatg
    31501 cttcgtcatt tcatgtaata acaggtcatt tgaagggtga tgatagtgga gaattaaact
    31561 ggaatgctct tgcaaataac aaaggtactc ttgtgttttt gatgggaatt tcaaacctag
    31621 agaaaataag tgaaaactta atgaaagaag gaaaagataa ggatacacct gttgctctta
    31681 taagttgggc aactagatat aatcaaagag ttgttacatc tacacttgag aatgtttatg
    31741 aaactgctat taaagaagaa gtaaaaccgc caactcttat agtagttggg agtgttgtag
    31801 gacttagaga aaagttgaac ttctttgaaa gtaagccttt atttggaaaa agtattgctg
    31861 taactaggtc tagaaatcaa aatagtgtat tggtagaaaa aataatggat ttaggtggaa
    31921 atcctataga aatacctact attaaggtag aaaaaataca aaataatatt aacttagaaa
    31981 atgaaataaa aaatataaat aagtataatt atttaatact aactagtaaa aatgccgtag
    32041 aaatcttctt tgaaaagata tatgaaatga attttgattt aaggatatta tctaatttaa
    32101 aggtatgtgc tataggttct gctacatcaa atgagttgaa aaaaagagga attatagcgg
    32161 atatagtacc aaaaaaattt gtagcagagt cactgtatga agagttagct cctattttaa
    32221 atgataacga taatatactt atacctagag cagaaaatgc tagagattat ctggttgaca
    32281 aacttaaggc tatttgtgaa gtaacagaag tacatatata tagaacagta atagatgagg
    32341 ataaaaaagg tgagatatta gatattttaa attctaatga tatagattat ataacattta
    32401 ctagttcctc aacagtaaat aatcttgttg aaattatagg aaaagaaaat attaaaatat
    32461 tagaaaaaat aaaaacacta tctataggtc ctataactac agagacaata aatagtttgg
    32521 gaataaaatt gtacaaagaa tctgctacat ctactataga ttcgttagta gatttaatta
    32581 taaaaaatta attttggagg cttgaatatg ttaagaagac caagaagact aagagcaagt
    32641 aaagcaatca gaaatttggt aagagaaact aaggtgaatg tagaagattt aatatatcca
    32701 ctgtttgtag tagaggggga aaatataaaa gaagaaatca attctttacc agatgtatat
    32761 catttatcaa tagacatgct agaagacgaa ataaaagaaa taagtgagtt gggaattgaa
    32821 catataatgt tatttggtgt tccagatgaa aaagatgagt gtgcttcaca agcttttaat
    32881 gataatggaa tagttcaaaa ggcaattaga aaggtaaaag aaataaattc agatataaat
    32941 gtaataacag atgtctgtat gtgccaatat acaagtcatg gacattgtgg gattttaact
    33001 gatactggtt atgttgataa tgataaatca ttggagtatt tggctaaaat atctgtaagc
    33061 catgctaaag caggagctga tatggttgct ccatctgata tgatggatgg aagaatacaa
    33121 gcaataagag aagctttaga tgaaaatgga ttagaaaatg ttggaatcat ggcttatagt
    33181 gttaagtatg cttcaaatta ttatggacct tttagagagg cagctcattc agcaccatct
    33241 tttggagata gaaaaacata tcaaatggac ccagcaaata gcaatgaagc tataatagag
    33301 actgaacttg atatattgga aggtgcagat atacttatgg ttaaacctgc attatcatat
    33361 ttagatatta taagaagagt taaagataat tttgacttac ctctagcagc ttataatgta
    33421 agtggagaat actctatgct taagtttgct gtgaaggaag gtttattaaa tgaagatgta
    33481 attcttgaat caattacatc tataaaaaga gcaggagcag atataataat aacttatttt
    33541 gctaaagaca ttgctaaact gttgaaaaaa taaattctag tagtataaag aatatcaaaa
    33601 agtataggaa ttgtaattaa ctattataca gtagttttgt ttagacattt attgaagaat
    33661 aatagaaact aatttacaat ttctatactt tatttatgta actaaataaa atttggaggt
    33721 agaagatgct atatcctatt aatttaaaat tagatgagtt agatgtagtt ataattggtg
    33781 gtggagaagt tgcatatagg aaatgtaaaa attttttaga gtttaacaaa aatgttacta
    33841 tagtttctaa acaaatttta aataaatttt atgatttaaa aggaaatata aaaataataa
    33901 aagatgatta taaagaaata tatataaaag atgcttctgt aataattgct gccacaaata
    33961 atagagagtt aaatatggaa ataggattat attgtagagc aaaaaataag ctagtgaatg
    34021 tagttgataa tatagaaata tcgaacttta ctgttccatc atatataaaa agaggagatt
    34081 tacttataag tgtgtctact gggggcaaga gtccttcatt atcatcaaag ataaaaaaag
    34141 aaattgaaga gagatatact gaagattatg aggagtattt aaatgtatta ggagatataa
    34201 gaaaaaaggt tataaaaaaa tacgaagata aaagtaaaag aaaagatgtg cttaatatgt
    34261 tggtaacact tgacctagaa gaacttaaga agtttgatat ataaaaaata aaataaaaaa
    34321 tgttaaaaag gtgttgacgg attattttta agatgatata gt
//
LOCUS       DAMJWP010000015        33082 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.016, whole genome shotgun sequence.
ACCESSION   DAMJWP010000015 DAMJWP010000000
VERSION     DAMJWP010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33082
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.016"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            131..271
                     /locus_tag="PC700_000678"
     CDS             131..271
                     /locus_tag="PC700_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580002.1"
                     /translation="MGIIESASKLAEMVHLLAVEKGITDIEAWDEAVKEYSKIYEERR
                     NE"
     gene            complement(446..766)
                     /locus_tag="PC700_000679"
     CDS             complement(446..766)
                     /locus_tag="PC700_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDF2580003.1"
                     /translation="MKLKTISLPELNNLDPTLESTFIKMGEEQGELAECIGKFRNLSG
                     ENNDLDEVDIIKKTAKELMDVAQTCVTMMFKLEEQYGINLDEIRKEHIKKLEKRGYIK
                     NMDK"
     gene            complement(886..1623)
                     /locus_tag="PC700_000680"
     CDS             complement(886..1623)
                     /locus_tag="PC700_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDF2580004.1"
                     /translation="MDANSLKDLIANHNNIVFFGGAGVSTESNIPDFRSSTGLFSQKL
                     NKQFTAEQLVSHTFFVRYPEEFFEFYKDKLIYPNAKPNNAHIALAKLEEMGKLKAVIT
                     QNIDGLHQMAGSKNVLELHGSVHRNYCTKCGKFFDLESMLNLGGNIPYCDNCGSIVKP
                     DVVLYEEALDSDVITKTISAISNADLLIIGGTSLAVYPAASFIDYYKGDYIALINKAN
                     TVYDKSASLVINKPIGEVLYEAVLRQI"
     gene            1838..3661
                     /gene="acd"
                     /locus_tag="PC700_000681"
     CDS             1838..3661
                     /gene="acd"
                     /locus_tag="PC700_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HDF2580005.1"
                     /translation="MKKKAALATLAMLPLGVVNAHADGDIGIVTINYLNVRNEPTAES
                     SIAFVAKKDDKVLIKDSSNGWYKIKAESGQEGWASSKYIAKSNSDSLRTSTNKEKQVI
                     SNSLNMRNGAGTSYRVITVLKKGQKVEVISESNGWSKIKYDGRLGYVSSSYLGDVSNS
                     TNKSKTKQVNTTSLNVRSGPNTSYGLLGKLPKGSKVEVISESNGWSKIKYNGKDAYIS
                     SMYLSDVSQSNSDNSSQSNDKKNTDKVVNTASLNVRSGPGSTYSKLGKVYKGSKVTVL
                     SESSGWAKINFNNKEAFVVGNYLSTSADTSNNNSNSNSDNSSNSNGNNSSSSGQVNGM
                     SGISGAKIDYKSLSYTLESHISKQVEKAASGGNVIAPSNRKSTPSPEFSTFSAQRTSS
                     FVNASSSDIEYYLNPKNFTNTTKGMMQFLKINSYRDGISESSLNSYLNGLSSSVFKNQ
                     GAAFINAAKKYNIDVVYLVSHAMWETAYGKSTLAQGQTLTSYKGQPLSKPVKVYNFFG
                     IGAIDKSANVSGAEAAYSNGWTSVEATIDGSAKWISQNYVNSSKYNQNTIYKMKWNYD
                     YTWHQYATDVNWANGISGIMENLIGLYGGGSSLVFEVPQYK"
     gene            3876..8174
                     /locus_tag="PC700_000682"
     CDS             3876..8174
                     /locus_tag="PC700_000682"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HDF2580006.1"
                     /translation="MESIKEYLDKLEINNSGLGKQLKEVYINRVVYFKEDKIVYFYLT
                     SKDIVSHELLDKFKEELMYKLDYFKDMRMKIRFTGLERKSNKDVIKKYWNNILYILKY
                     LCPSIAGWYKQVEFLCLEEELKIKLPKGIFYERLMKKNVTYVLKTVLSEELGLDLNIT
                     IEKAVDEKVNKERLIRINDREMEEKIRALEIGKVNNCEENEEESYVIKSDVDENLIYG
                     DNANAMIENIVELNASSGTVAVVGDIFDVDTKELKNGKILMIASITDYTSSISCKLFL
                     TDTNKDGVLENVKKGAYLKIKGDIVYDTYQREISMMISGIRKETRIEREDKSEEKRVE
                     LHAHTQMSSMDAICSVKKLVERAAKWGHSAIAITDHGVVQGFPDAMNAGKSNNIKILY
                     GVEGYLVEDDSLIIEDANDKELSQTFVVFDIETTGFSNTNDKITEIGAVKIENFKIVD
                     RFSELINPEKDISYKIQELTGITNELIKDKPTIEEILPKFMEFVGDSVLVAHNAEFDT
                     GFISQKCREQGLLYNNKKVDTLMLARVMLPNLKRHRLNVVAKELGIPLLNHHRAVDDA
                     EATALIFNKFLQMLTEKGAKTLSDVNNILGKIDYTKLSTNHITLIAKNSIGIKNLYKI
                     ISDAHVNHFFRAPRILRSVLNEYKEGLIIGSACEAGVVFQAVKKNVSDEEMKKIIDLY
                     DYIEVMPIDNNRFMIDKGEVKDEEELRELNRKLIDTAKKFDKIPVATGDVHFLDKHEA
                     VLRKVLKYSQGFKVDEEETYLHFRTTDEMLEEFSYLGEELAYEVVVENSNLIADMVED
                     IKPIPDDTYPPIIEGSDVELREMCYKKAKRIYGDPIPEIVQKRLDRELNSIISNGYAV
                     MYIIAQKLVAKSLSDGYLVGSRGSVGSSFAATMSDITEVNPLPAHYICENEDCKYSFF
                     YEIGEWGSGVDLPDKDCPKCGRKLAKNGHDIPFEVFLGFEGDKEPDIDLNFSGNYQPT
                     IHKYTEELFGEGYVYRAGTIGTVAEKTAFGYARKYVEENNISVPNAEVLRLSNGCTGV
                     KRTSGQHPGGVMVIPDYKDVYDFTPIQYPANDTSCGVITTHFDYHSISGRILKLDILG
                     HDGPTIIRMLEDMTGINITEIPLDDKETMSLFTSTEALGVTPEEINCPIGCLAVPEFG
                     TKFVRQMLLDTKPTTFAELVRISGLSHGTDVWVNNAQDLVREDIVGLKDVISTRDDIM
                     NYLIFKGLPPKMSFTIMESVRKGKGLKPEHIEEMKKNNVPEWYIGSCKKIKYMFPKAH
                     AVAYVMTSFRIAYCKVHYPEAFYATYFTTKVEDFDADLIVKGLDAIKSKINEIESLGN
                     DATTKEKGMLTVLEVALEMYARGIKLLPVDIYKSDATEFIVVGEKTLLPPMAAIQGLG
                     GNAAINIQNERKNGEFISKEDLRKRTKISKTVIETLTNHGSLENMSERNQLSLF"
     gene            8378..8839
                     /locus_tag="PC700_000683"
     CDS             8378..8839
                     /locus_tag="PC700_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDF2580007.1"
                     /translation="MKKNLEATIEEIVTKITDEHGFEMVDVEYVKEAGEYYLRVYIDK
                     EEGISLNECELVSRELSPILDEKDPIKENYFLEVSSPGLDRALKKDRDFVRYQGRDVD
                     LKLYKPLNGCKQFEGELVGLTEDNNIKIIANGKEMEFNRKDVAIVRLAIKF"
     gene            8869..10023
                     /gene="nusA"
                     /locus_tag="PC700_000684"
     CDS             8869..10023
                     /gene="nusA"
                     /locus_tag="PC700_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDF2580008.1"
                     /translation="MNHEFMEALDELVVDRGIDKEILIDTIEQALLTAYKKNFGSAQN
                     VRVEFDRDKGDIKVFSQRVVVDESDLYDTFLEIELADAREINPNYELGDIIEHEVTPK
                     NFGRIAAQTAKQVVVQRIREAEREIVYNEFIEKENEIVTGEVARANKNVVYVNLGRIE
                     GIMTQSEQIPFENYKAGQKIKFYILEVKKTNKGPQIVVSRSHPGLVKRLFEEEVPEIY
                     EGIVQIKSVAREAGSRTKMAVKSIDEKIDPIGACVGPKGLRVKNIVDELGDEKIDIIK
                     YSEDPAEFISAALSPSKVVRVDIDEDEKSALVIVPDYQLSLAIGKEGQNARLAAKLTN
                     WKIDIKSESQAEQEMLNLENSKEIEAEDVEAEFIDQEELGTSEDNFSEEE"
     gene            10030..10311
                     /locus_tag="PC700_000685"
     CDS             10030..10311
                     /locus_tag="PC700_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HDF2580009.1"
                     /translation="MILKKVKKVPQRKCIACQDRDSKKELIRIVKNKEGKIFLDPTGR
                     ANGRGAYVCKSSECLKKAIKSKALNKAFKIDVPDEVYDNLLMELEEYEK"
     gene            10301..10612
                     /locus_tag="PC700_000686"
     CDS             10301..10612
                     /locus_tag="PC700_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HDF2580010.1"
                     /translation="MKNNKEKVLSFLGLATRAGKIVSGDDSTLLDLKKGKVNLILIAE
                     DASNNTKKLFKDKSTFRNIPYLFFSTKEEIGFAIGKSPRAVVGIKDENFSKKIIELIE
                     I"
     gene            10634..12574
                     /gene="infB"
                     /locus_tag="PC700_000687"
     CDS             10634..12574
                     /gene="infB"
                     /locus_tag="PC700_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDF2580011.1"
                     /translation="MSKTRVYQIAEELNISNEELINKLAELDINVTDKDSVLEGEELE
                     LALEMLGEDLSQENGNVIEIDGKLTVQVLATKLDKSPSEIIMKLMKMGTMATINQEIS
                     FEIAALAAKDYGFELTVAESDDTEALEIEALMEIEEDKEEDLKPRPPVVTVMGHVDHG
                     KTSLLDAIRKTDVISGEAGGITQHIGASEVKINGHKIVFLDTPGHEAFTSMRARGAQV
                     TDIAILVVAADDGIMPQTVEAINHAKAAGVPLIVAINKIDKPGANPDKVKQELADQGL
                     LVEDWGGEVIAVPVSAKKKEGIDTLLEMVLLVAEMEELRANPNKRAVGTVIEAELDKG
                     RGPVATVLVQGGTLTVGDPIVAGVACGKVRAMINAKGKRVKTAGPSTAVEILGLSEVP
                     QGGDQFVEVPTDKIARSVAARRQQIVRDEMLKSTQRLSLDALFSQMSEGSIKDLNIVI
                     KADVQGSVQAVKQSLEKLSNEEVQVKVIHGGVGAVTESDILLAAASNAIIIGFNVRPV
                     PGAESLGEKENVDIRTYTIIYKAIEDIQAAMTGMLDPEYVDEETGKAEIREIYKISGV
                     GTVAGCYVTNGKIFRNCKVRLVRDSIIIHEGELAALKRFKDDVKEVNSGYECGMSFVN
                     YNDIKEGDIVEAYITKEVERKL"
     gene            12617..12997
                     /gene="rbfA"
                     /locus_tag="PC700_000688"
     CDS             12617..12997
                     /gene="rbfA"
                     /locus_tag="PC700_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDF2580012.1"
                     /translation="MASYNRTRRIAEEIRKVVSTMLINGVKDPRITSMVSVTDVEVTN
                     DLRYAYVYVSILGGDEESTLTGLKSAGGYIRREVGKNIKLRYIPEIVFKLDDSIEKGM
                     YMDNLIKRVNEKNAQQNEDENYDE"
     gene            12978..13961
                     /locus_tag="PC700_000689"
     CDS             12978..13961
                     /locus_tag="PC700_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HDF2580013.1"
                     /translation="MKIMMNNMDSIINYIYEGDYFIVTSHISPDGDNVGSTLSMYYTL
                     KNLGKNVYYVLDDEAPLNLRFLVEGVKIYKSNEFNMKNYSLIALDCGDKMRICVSDEI
                     KNNASKLICIDHHASNDSYGDLNYVDIDASSTCELVYNLLVRFQQTKDINIINEDIAT
                     CLYTGLVTDTGNFSYSNVHASSFEMAKNLLVLGAQKNTIIQNIYQSNSSDYYKLLGEA
                     LKGLEIFDSKVSSIVLTQDMMNRNNISFNDVDGITTYTRDIKGIEVGILFKEKKQNEI
                     KVSFRSKNYVDVSEIAKLFGGGGHVRAAGCTIRDSIDNAKKMVLEAVLKSI"
     gene            13974..14879
                     /gene="truB"
                     /locus_tag="PC700_000690"
     CDS             13974..14879
                     /gene="truB"
                     /locus_tag="PC700_000690"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDF2580014.1"
                     /translation="MNKIISILKPTGMTSHDVVSRVRKILNIKKVGHTGTLDPDASGV
                     LPICIGKATKVCEVILNKDKSYICELTLGISTDTYDASGEILKKVDDFKFSNEDIERA
                     FDTQRGEINQLPPIYSALKVNGKRMCDLVRSGRQSEITLKTRRVNIKDIKILSIKGNK
                     VMFYVECSKGTYVRSICHDIGEYLGCGAHMSFLNRTSSGKFDLDNSITLEELELFYEN
                     KTLDKYLYDIDYVLDSFNYVVLNPNAIKYYSNGGSIDDKRFLKNNFDKDDEVVRVYST
                     DNFLGLGKLSKHNNTISVKSDKMFI"
     gene            15010..15939
                     /locus_tag="PC700_000691"
     CDS             15010..15939
                     /locus_tag="PC700_000691"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HDF2580015.1"
                     /translation="MVIIKSIKEIANIKESVITIGNFDGVHKGHQVLIGKTVEYAKKE
                     NIQSIVFTFANHPVNYFRPNSTKNIISNDDKIKLLEDLGIDIVVIIPFDEYMTKIPAK
                     DFVDEILVKKLKAKKIVIGHDFTFARAKEGNVNLLKSLEHEFGFEVEVIKPIKINDVR
                     VSSTYIRSLVSQGDMANVKEYLGRNYKLEGCVIHSKHLGRTIGFPTANIDLKNNMLVP
                     KRGIYASIVHIGDETYFGATNVGYNPTVNGKTLSIETNILEFDRDIYGENIIVEFLER
                     IRDERKFNSIDDLKNQLYNDTNYVYENYVCKNK"
     gene            16043..16300
                     /gene="rpsO"
                     /locus_tag="PC700_000692"
     CDS             16043..16300
                     /gene="rpsO"
                     /locus_tag="PC700_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDF2580016.1"
                     /translation="MINKSEIIKEYGRAEGDTGSPEVQIALLTARINELNGHLKTHKK
                     DHHSRRGLLKMVGRRRNLLAYLKEKDLEGYRALIAKLGLRK"
     gene            16540..17385
                     /locus_tag="PC700_000693"
     CDS             16540..17385
                     /locus_tag="PC700_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDF2580017.1"
                     /translation="MKIKLVCDSLCDIPDEISEKDYIEIVPLTVIFNDREYIEGVDIK
                     KEEFYKKVKEIKQIPKTSQATYMEFKEVFDKFITEGYHIICMTGAANASGTFQSAMIA
                     KNDVNENEKIHIFDTRNLSLGSGQYVIKACELLEEGLGFEEIIDELENTRSSVRLLFA
                     PYTLDFLKQSGRVPVATALIGNMLNIKPIFFFDNGEAKLVNKVRGIKNIASKLVDIIL
                     EMNEGSLEGKIVTIGCGDNLHDCEILKDEVNKRIKARRVLFTRGGVSICSHTGPDILA
                     ISCSN"
     gene            17510..19621
                     /gene="pnp"
                     /locus_tag="PC700_000694"
     CDS             17510..19621
                     /gene="pnp"
                     /locus_tag="PC700_000694"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDF2580018.1"
                     /translation="MFEHKIFKMDFAGRELSVEIGKICEMASGSCIVRYSDSMVMVNT
                     TKSAKPRDGIDFFPLSVDYEEKLYSVGKIPGGFLKREGKPSEKAILTSRLIDRPIRPL
                     FPKGFRNDVQVVATVLSVDQDCTPDIVAMIGSSIALSISDIPFNGPTGSVCVGLVDGA
                     FVVNPNAEQREKSSMHLVVSGTKEAIMMVEAGADEVPDEVMLDAILFAHQEIKKIVEF
                     IEGIVAEVGKEKMPVELYHAGEEITQLVREFATDKMKKAVQTFEKLERMENMDRVKEE
                     TLAHFEETLEDFEDFVGDIEEVLQDIIKEEVRKLIVHENVRPDNRKLEEIRPIWCETG
                     MIPRAHGSAIFTRGQTQVLNVATLGALGDVQKLDGLDEEENKRYMHHYNFPAYSVGEA
                     RPSRGPGRREIGHGALAERALLPVIPSQEEFPYAIRLVSEVLSSNGSTSQASVCGSTL
                     SLLDAGVPIKDMVAGIAMGLIKHDGKVAVLSDIQGMEDHLGDMDFKVAGTEYGITAIQ
                     MDIKIDGIDKEILQRALKQAKEGRIHILGEMRKTISQPKPELSPYAPKIVKMQINPDK
                     IKDVIGPGGKIITKIIDETGVKIDIEQTGEVFISGIEIDMIKKAQELINNIVVEPEVG
                     KTYKGKVSRIMNFGAFVEILPGKEGLLHISHIAHERVAKVEDVLNIGDEVEVKVTEID
                     EKGRVNLSRKVLLPKPEHKNK"
     gene            19887..20630
                     /locus_tag="PC700_000695"
     CDS             19887..20630
                     /locus_tag="PC700_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2580019.1"
                     /translation="MIMMVLNKSKLKKIIILILVVLLVLSGGVMVLKLFNNKPVFNLF
                     NGVDLPYERGTKDKSGYVALTCNIDLGWETEYVESILETLKKENVKITFNVTGKWAEK
                     NKDELLKIKKQGHEIGNHGYKHLDYSTLSYEDNYEQIETSKKIIEEIIGEKTKFFQAP
                     AGSFGPETVKAAKALGYTSIKWDADTIDWKYKDQPEVIIDRMKKKDIKDSSIILMHPT
                     NATTKCIDDIIAIVREKGLKPGKLSDVFK"
     gene            20788..22035
                     /locus_tag="PC700_000696"
     CDS             20788..22035
                     /locus_tag="PC700_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDF2580020.1"
                     /translation="MYKTKILENGLTIIGEEIPYLKSITLGIWINAGSRIEEAQVSGT
                     SHFIEHMMFKGTKNRTSKEIASSIDNLGGQINAFTSKECTCYYVKLIDEHIDTGIDVL
                     SDMILNSKFDKNDIDKERLIILEELKMYEDSPDDLSYDLLVENIYANDGLGMNIIGTK
                     ESLYNITRESMLEYLNKYYIPNNAVISIAGNFNFDDMVEKIKSKFGHWEKKNLSIDIS
                     EAKFNPCFISKNKDTEQVNLAICLKGIPFENDEEVYSMAVVNNIFGGSISSRLFQKIR
                     EEKGLVYSIYSSQTLYRKCGELGIFASMSTENLQDVYNLIKKEIENIRENYLTEKEIS
                     ESKEQLKGNYILDLESTSSRMMSTGKSMLLSKKVKTTDEILECINNVNINSIKKVVDK
                     VFNIENIGTCIVGRDVEKILHLD"
     gene            22070..22315
                     /locus_tag="PC700_000697"
     CDS             22070..22315
                     /locus_tag="PC700_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HDF2580021.1"
                     /translation="MNLSEIAGKEIVNLVTGERLGVIGECDLILDETTGGILALLIPK
                     EKGFFARRKDKSFLEVPWRNVKKIGNDMIIIEYEGYI"
     gene            22325..23548
                     /gene="dapG"
                     /locus_tag="PC700_000698"
     CDS             22325..23548
                     /gene="dapG"
                     /locus_tag="PC700_000698"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDF2580022.1"
                     /translation="MSILVQKFGGTSVESYEKMNEVCKIVKAYKKNDEELQLVLVVSA
                     MGRKGAPYATDTLINLCSAVNDEPSKRELDLIMSCGEIISGTILANLLNAQGIDSVFF
                     TGQQAGIITSDEYSNAKIKYINPKKVKRALDDGKVVVIAGFQGVTDDGEITTLGRGGS
                     DTSAVAIGKALECETVEIYTDVDGIMTADPRVEPNAKVLSFIDYEEVFQMADKGAKVI
                     HPRAVQLAKSGNITLAIKNTMNPTFEGTKIGSLCRHLEENIEYEQERDFKVAVANKDS
                     VAQVKIKSAEEVFTEVLNEIEKKLITIDMINFFISEKAFVVEDADIKSLKEILDKFEL
                     DYEVNRDCAKVTLICSRIADEMSGIMSKVVRGLSKAGVSLLQTSDSNMTISCLVSEED
                     MHTAVHAIHQQFYLK"
     gene            23661..24395
                     /locus_tag="PC700_000699"
     CDS             23661..24395
                     /locus_tag="PC700_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HDF2580023.1"
                     /translation="MKDYREDNERFDKDNCSEESSCEERECIKQFGTNEMPPQPPKDI
                     QCITIIGEIEGHFIGNPQKKATKYEHIIPMLYSIEESNDVKGVLVVLNTVGGDIEAGL
                     AIAELLNSTSKKVVTLVLGGSHSIGVPLATAGDYSFIAPTATMIIHPVRTTGLVIGIN
                     ETFEYFKKMQDRIIQFIIRTSNIKKDVLEKLMHEKDELVSDVGSVLIGKEAVDYGLID
                     EVGGLKEALKKLRELIKESEEEKNND"
     gene            24518..26929
                     /locus_tag="PC700_000700"
     CDS             24518..26929
                     /locus_tag="PC700_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDF2580024.1"
                     /translation="MATKKKKKKKVNTLSFNAEYHNLITIFIGVFLLYSLNSNSMGWI
                     PVLMQNLFKGLFGGLSIAIPFIVIITGLLGFFDGNEYIYRLRKTKLYYIIILFIFVFY
                     GLLNAGTLPVDSPLKGNMFDDVMKLGVSGQGSGLIATTIAYYMSKIFGIAGGWLISIF
                     ALILSVMFIFNISIKDLVSNAKSKSKASKDSNLTFKDKIANMKKSAIDMMTDEVDDTT
                     INKKPGFFKGLMSKGLNNDDEEDEYFEEENSDGVDDKTIKIVGFNKAEDEYLEILEGT
                     QSMPELDVLKELQKATNENPVVDTKPEKKVDIAKSNLNIEKTQPMSIVAEPVNEDYSN
                     YKKPSIELLNKVNKKSDENGKKKVLKNASLLEKTLSDFGVEAKINQVTVGPTITRYEI
                     QPSPGVKVSKIVNLTDDIALSLAAKSIRIEAPIPGKSAIGIEVPNEEAQMVGVREVLE
                     SEEFNNFNSPLAMGLGKDVAGKIIIGDIGKMPHLLIAGSTGSGKSVCVNTLISSILYK
                     ANPDEVKLLLIDPKVVELANYNGIPHLLIPVVTDPKKAANALNWAVTEMNRRYKLFAD
                     AQVKDVTSYNEKAEEKLPKIVIIIDELADLMMASANDVEDYICRLAQMARAAGMHLIV
                     ATQRPSVDVITGVIKANIPSRIAFAVSSQTDSRTILDMGGAEKLLGKGDMLFYPLGAA
                     KPVRLQGAFISESESEKVIDFVKSQVKDGIKYEEDIIETISKVNTSKSSDEDEFLSEA
                     IEFVVESGQASASMLQRRFKIGFNRAARLIDSMEERGIIGASEGSKPRKVLISKQDLQ
                     NLEGE"
     gene            26932..27990
                     /gene="mnmH"
                     /locus_tag="PC700_000701"
     CDS             26932..27990
                     /gene="mnmH"
                     /locus_tag="PC700_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HDF2580025.1"
                     /translation="MSVIKIEKALKLDKVIFVDVRTEGEYEEDHILNAINMPLFKNNE
                     HNEVGTIYKMQGKHEAIQKGFDYVSYKLKDIYLQAAELALNYDNIVIYCARGGMRSGS
                     IVNLLSSLGVNVYQLEGGYKAYRNFVLEYLRHVMDTKNFVSVHGLTGTGKTDLLHLLD
                     EKGIDVLDLEGIAKNSGSTFGFITFDKKPPSQKNFETKLFESVFFSKENYIFVESESK
                     RVGSVTVPNEIFEAMTREGHHILLECDIENRVDRLCRDYIYDKDEGNILILKECINKF
                     RKRLGNKVVDDYIDLLESGEYKELVKRYLLEYYDPLYMHSVEKYKYSKIINFNDTQKA
                     LEDVIDLYESILEGESEC"
     gene            27984..29318
                     /gene="rimO"
                     /locus_tag="PC700_000702"
     CDS             27984..29318
                     /gene="rimO"
                     /locus_tag="PC700_000702"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HDF2580026.1"
                     /translation="MLKIALESLGCSKNLVDAEIMMGILNNKGYKLIGDFEEADVIIV
                     NTCGFIESAKQESIDTIINFSELKKTGNLKLLIVTGCLAQRYSEELKTEIPEIDAIVG
                     TGSYQNIDKILKELSEMHQIVSLNDIEFVFNEDLPRYISTPSYMAYLKIGEGCSNNCT
                     YCIIPKLRGKYRSRKFEDIIKEAKKLAESGVKELVVIAQDTTKYGFDLYGKERLSELL
                     EELAKIDGFKWIRVMYSYPESITEELIQVIKKYDNICSYFDMPIQHASNNILKLMNRK
                     TTKEDILNKINLIRSNIPDAILRTTIIVGFPGETEDDFKQLVDFVEEVKFDRLGAFAY
                     SREEDTPADRLPNHIDEEVKIQRRDTLMMIQQKISEELNDKKIGKTYEVLIEEQIEDN
                     VYTGRTQGDAEEIDSIVYVKSVDNLEVGEFVSVQINDAMEYDLMGDVLYELA"
     gene            29305..29847
                     /gene="pgsA"
                     /locus_tag="PC700_000703"
     CDS             29305..29847
                     /gene="pgsA"
                     /locus_tag="PC700_000703"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDF2580027.1"
                     /translation="MNLPNKLTLFRIFLIPVFVLIMLLNVPNKFLIACIIFIIASITD
                     ALDGKIARKYNLVTDFGKFMDPLADKLLVISALTCMIEDHLVSSWMVIIIVARELTVS
                     ILRAIAAADGKVIAAGNSGKLKTITQMVSIVFLLLGAQFENVLILNIGEILILIATLL
                     TLYSGWEYLYKNKELFMSSK"
     gene            30135..31181
                     /gene="recA"
                     /locus_tag="PC700_000704"
     CDS             30135..31181
                     /gene="recA"
                     /locus_tag="PC700_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDF2580028.1"
                     /translation="MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTG
                     AIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSCVASAQKDGGIAAFIDAEHALDP
                     VYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
                     GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKF
                     YSSVRLDVRKIDTIKQGDKVIGSRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLL
                     DIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTTEIDEKVRAFYNLNEEH
                     EESGTSVSKEIVEE"
     gene            31357..32898
                     /gene="rny"
                     /locus_tag="PC700_000705"
     CDS             31357..32898
                     /gene="rny"
                     /locus_tag="PC700_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HDF2580029.1"
                     /translation="MDVIDSVVMIVIGAAVGIIAGYFVRKNISEAKIGQAENLAKEII
                     DKAHHDSETVQKEKLLEAKEEIHKWRTEAERENRERRTEVQKYEKRVVQKEEVLDRKL
                     QNLESKEVNLSEKLKVVEKKEEEVEVIKTQQLEKLESISGITSDKAKEIILTNAERDV
                     RREMSIMIKEIESQAKEEADKKSREIIGYAIQKCAADHVAETTVTVVSLPNDEMKGRI
                     IGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARIALEKLIADGRIHPAR
                     IEEMVEKARKEVDNIIKEYGEQAAFETSVHGIHPELIRLLGRLNYRTSYGQNVLKHSI
                     EVAHIAGIMAAEIGADIRLAKRAGLLHDIGKAVDHEMEGTHVEIGMDLLRRYKESKEV
                     IHAMSTHHGDYEPQTVEAVLVTAADAISAARPGARRETLEAYIKRLEKLEEIANSYEG
                     VEKSFAIQAGREIRIMVKPEAINDEEIHLLARDMTKKIEDELEYPGQIKVSIIRETRA
                     IEYAK"
BASE COUNT        13399 a         3512 c         6335 g         9836 t
ORIGIN      
        1 tagatatatc ctaattatgt caaaaaataa aaaaattttt atttattaga gtttttggaa
       61 ataataataa cagatattat aattaattaa cagtttttgt aagtatttga caatagagag
      121 ggtgtaacaa atgggaataa tagagtcagc atcaaaattg gctgagatgg ttcatttatt
      181 ggctgttgaa aaggggataa ctgatataga agcatgggat gaagctgtga aagaatacag
      241 caaaatttat gaagaaagaa gaaatgaata gaatgataaa aaatgaaaac ttgatgataa
      301 aaaataactt tatttaaaga aattcaattt ttatagaaat attaaaaata atataaataa
      361 aaagatggta tttcaaatgt aagtaaaaaa tcattttggg ataccatcct tattatttat
      421 tttaataata tataagtaat aaatcttact tatccatatt ttttatgtaa cctctttttt
      481 ctagcttttt tatatgctcc ttacgtattt catcaagatt aataccatat tgttcttcta
      541 gcttaaacat catagtaaca catgtctgag ctacatccat tagttcctta gcagttttct
      601 ttataatatc aacttcatct aaatcattat tttctccact tagatttctg aatttaccta
      661 tacattcagc taattcacct tgttcttcac ccattttgat aaatgtagat tctagtgttg
      721 ggtctaaatt attaagctct ggtaaagata tagtttttag tttcataaaa gctcctttca
      781 agttattcgt atttatacta aaacatcata acaaaaataa tactaaaaaa aaactttaaa
      841 ttcaacataa aaacttacat atataataaa aatcgtgaaa taaatctata tttgtcttaa
      901 tacagcctca tataaaactt ctccaatagg tttatttatt acaagagaag ctgacttatc
      961 atagactgta tttgctttat taataagagc aatataatca cctttgtaat aatctataaa
     1021 acttgctgct gggtacacag ctaatgatgt acctcctata atcaataaat cagcattact
     1081 tatagcagaa attgttttag taatgacatc actgtcaaga gcttcctcat aaagtactac
     1141 atctggtttt actatagaac cacagttgtc acaataagga atattacctc ccagatttaa
     1201 catagattct aagtcaaaaa acttaccaca tttagtacaa taatttctat gtacagaacc
     1261 atgaagttct agcacatttt tacttccagc catttgatga agtccatcaa tattttgagt
     1321 tatgacagct ttaagcttac ccatttcctc gagttttgct aaagctatat gagcattgtt
     1381 gggttttgca ttaggatata ttaatttatc cttataaaat tcaaaaaatt cttctggata
     1441 tctaacaaaa aaagtatgtg aaactaattg ttcagcagta aattgtttat tgagtttttg
     1501 gctaaataat ccagtagaac ttctaaagtc tggaatatta gattcagttg aaacacctgc
     1561 tccaccaaaa aatacaatat tattgtgatt agctattaag tcttttaagc tatttgcatc
     1621 catagttaca cctccaaatt attatatata aattgtacta catattaggt cttttatgtt
     1681 ttattaattt aaataaatat gtagtttaat aagttaaata aagggaatat aaataaaatg
     1741 tatacagagt ataaatagac aatatttact aaaataacaa gttgtcgtat aattatatta
     1801 aaattgtttt agtgtataag aagggcgggg gaagtacgtg aagaaaaaag cagctttagc
     1861 aacattggct atgttaccct taggtgtggt aaatgcacat gctgatggag atataggtat
     1921 agtgactata aattatttaa atgtaagaaa tgagccaact gccgaaagta gcatagcctt
     1981 tgttgctaaa aaagatgata aagttttgat taaagactct tctaatggat ggtataaaat
     2041 aaaagctgaa tctggacaag aaggttgggc ttcatcaaaa tatatagcaa aatcaaatag
     2101 tgactcttta agaacgtcta caaataaaga aaaacaagtt atttcaaata gtttaaatat
     2161 gagaaatgga gcaggtacaa gttacagagt aataactgta ctaaaaaaag gtcaaaaagt
     2221 agaagtaata tcagagagta atggatggtc taaaatcaaa tatgatggaa gactaggata
     2281 tgtatctagc tcttatttag gagacgtttc aaattcaact aataaatcaa aaactaaaca
     2341 agtgaataca acttcactaa atgtaaggag tggaccaaat acaagttatg gtttgttagg
     2401 caagttgcca aaaggaagta aagtagaagt aatatcagaa agtaatggat ggtccaaaat
     2461 aaagtataat ggaaaagatg cctatatatc tagtatgtac ttatcggatg taagtcaaag
     2521 taattcagac aattctagtc aaagcaatga taaaaagaat actgataagg ttgtaaatac
     2581 agcttcttta aatgtaagaa gtggaccagg ttctacatat agtaagttgg gaaaagttta
     2641 taaaggaagt aaagtaactg tactatcaga aagtagtgga tgggctaaga ttaattttaa
     2701 caataaagaa gcatttgtag taggcaatta tttatctact tcagcagata cttcaaataa
     2761 taactcaaat agtaactctg ataacagttc taatagtaat ggcaataatt cttcatcatc
     2821 aggtcaagta aatggtatgt caggtataag tggggctaaa attgattata aatctttgag
     2881 ttatacttta gaatctcata taagtaaaca agttgagaaa gcagcatcag gaggaaatgt
     2941 gatagctcca agtaatagga aaagcactcc aagtcctgaa tttagtactt tttcagctca
     3001 aagaacaagc tcatttgtaa atgcaagttc aagtgatata gaatattatt taaatcctaa
     3061 aaattttaca aatactacta aaggtatgat gcagttttta aagattaaca gttatagaga
     3121 tggtatttca gaatcaagtc ttaactcgta tcttaatggt ttatcttcta gtgtatttaa
     3181 aaaccaggga gcagccttta taaatgctgc caagaagtat aatatagatg ttgtatatct
     3241 agtatctcat gctatgtggg aaactgctta tggtaagtct acacttgccc aaggtcaaac
     3301 attaacatct tataagggac aacctcttag taagccagtt aaagtataca atttttttgg
     3361 tataggagca atagataaaa gtgctaatgt ttcaggtgca gaagcagcgt attccaatgg
     3421 ctggactagt gtagaagcta caatagatgg ctctgcaaag tggatatcac aaaattacgt
     3481 aaatagttct aagtacaatc agaatactat ctacaagatg aagtggaatt atgattatac
     3541 atggcatcag tatgcaactg atgtaaactg ggctaatgga atttctggaa ttatggaaaa
     3601 tttaattggt ctttatggag gaggaagtag tttggttttt gaagttcctc aatataagta
     3661 atacataaat aaaaaatata gactatgggt agttttcatt ttatttaaat tatctatagt
     3721 ctttttgtat ataatttata atctgtaaat ttaatgttaa caaaactttt atgttataat
     3781 attaattcta agtaaatata agtgagtaat aggtagaaat agataaaaag tggtaataat
     3841 atatataaag ataattttga gaaaggagga tatatatgga aagtattaag gaatatttgg
     3901 ataagttaga aattaataat agtggtcttg gaaagcaact taaagaagtt tatataaata
     3961 gagtagttta ttttaaggaa gataagatag tttattttta tctaacttct aaagatattg
     4021 tttctcatga acttttagat aaatttaaag aagaattgat gtataaactc gattatttta
     4081 aagatatgag gatgaagata agatttactg gtcttgaaag aaaatccaat aaagatgtta
     4141 taaaaaaata ttggaataat atattgtata tattaaagta tctttgtcca tctattgcag
     4201 ggtggtataa acaagtagaa tttctttgtt tagaagaaga attaaaaata aaacttccaa
     4261 aaggtatatt ttatgagcga ttaatgaaaa aaaatgtaac ttatgtactt aaaaccgtgc
     4321 ttagcgaaga gttgggatta gatttaaata taacaataga aaaagctgtt gatgaaaagg
     4381 taaataaaga aagactcata agaatcaatg atagagaaat ggaagaaaaa attagggctt
     4441 tagaaattgg aaaagttaat aactgtgaag aaaatgaaga ggaaagctat gtcattaagt
     4501 ctgatgtaga tgaaaattta atctatggag ataatgcaaa tgctatgatt gaaaatatag
     4561 ttgaattaaa tgcatcttct ggaacagttg cagttgttgg agatatattt gatgttgaca
     4621 ctaaagaact taaaaacgga aaaatactta tgatagcttc aattacagat tatactagct
     4681 caataagctg taaacttttt ttaacagata caaataagga tggagtactt gaaaatgtta
     4741 aaaagggagc atacttaaaa atcaagggtg acatagttta tgatacatat caaagagaaa
     4801 ttagtatgat gattagtggg ataagaaaag aaactagaat agagagagaa gataaatcag
     4861 aggaaaaaag ggtagaactc catgcacata cacaaatgtc ttctatggat gctatttgtt
     4921 ctgttaaaaa gcttgtagag cgtgcagcca aatggggtca ttctgctatt gctataacag
     4981 accatggtgt tgtacaagga tttccagatg ctatgaatgc aggtaagtca aataatataa
     5041 aaatacttta tggagtagaa gggtacttgg ttgaagatga ttctctcatt atagaggatg
     5101 caaatgataa agaactttcg cagacatttg ttgtatttga catagagaca actggttttt
     5161 ctaatacaaa tgataaaata actgagattg gtgctgtaaa aatagaaaac tttaagattg
     5221 tagatagatt tagtgagtta ataaatcctg aaaaagatat ttcctataag atacaggaat
     5281 tgacaggaat aactaatgaa ttaattaaag acaagccaac cattgaagaa attcttccta
     5341 agtttatgga atttgttgga gatagtgtat tagtagcaca taatgcagag tttgataccg
     5401 gatttatatc gcaaaaatgt agggaacaag gcttgttata taataacaaa aaagtagata
     5461 cacttatgtt agcaagagta atgttgccaa acttaaaaag acatagatta aatgtcgttg
     5521 caaaagaact tggtattcct cttttaaatc accatagagc agtggatgat gctgaggcta
     5581 cagcacttat atttaataag tttttgcaga tgcttacaga aaaaggtgca aaaactctaa
     5641 gtgatgtaaa caatatactt ggaaagattg attacacaaa gcttagcaca aatcatataa
     5701 ccttgatagc aaaaaattct ataggtataa aaaatttata taaaataatt tcagatgcac
     5761 atgtaaatca tttttttaga gcaccaagaa tccttagaag tgtattaaat gaatataaag
     5821 aaggattaat aattggttcg gcttgtgaag ctggggtggt ttttcaagct gtaaagaaaa
     5881 acgtgtctga tgaggaaatg aaaaaaataa tagatttata cgattatatt gaagtaatgc
     5941 ctatagataa taatagattt atgattgata aaggagaagt aaaggatgaa gaagagttaa
     6001 gggagttgaa taggaaatta attgatacag ccaaaaaatt tgacaaaatt ccagtagcta
     6061 ctggggatgt tcatttttta gacaaacatg aagctgtctt aagaaaagtt cttaaatatt
     6121 ctcaaggttt taaagtagat gaagaagaaa cttatttaca ttttagaaca acagatgaaa
     6181 tgttggaaga gtttagttat cttggagaag aattggcata tgaagttgta gttgaaaata
     6241 gtaatctgat tgccgatatg gttgaagata taaaacctat accagatgat acttatcctc
     6301 caattataga gggctcagat gtggagttaa gagaaatgtg ttacaaaaaa gccaaacgaa
     6361 tctatggaga cccaatacca gaaatagttc aaaaaagact ggatagagaa ttaaattcaa
     6421 taataagcaa tgggtatgca gttatgtata ttatagctca aaaactggtt gcaaaatctc
     6481 tttcagatgg atatttagtt ggttcaagag gctcagttgg ctcatctttt gcagctacta
     6541 tgagtgatat aacagaagtt aatcctcttc cagcacatta tatttgtgaa aacgaagact
     6601 gtaaatattc attcttttat gaaataggtg aatggggctc aggtgttgat ttaccagaca
     6661 aggattgtcc aaagtgtgga agaaaacttg ctaaaaacgg tcatgatatt ccatttgagg
     6721 ttttcttagg ttttgaaggt gataaggaac cagatataga cttaaatttc tcaggaaatt
     6781 atcagccaac aattcataaa tacacagaag aactatttgg agaggggtat gtatataggg
     6841 ctgggactat aggtactgta gcagaaaaaa cagcttttgg atacgcaaga aaatatgtgg
     6901 aagaaaataa tataagtgta ccaaatgcag aagtacttag gctttcaaat ggatgtactg
     6961 gagtaaaaag aacctcagga caacatcctg gtggagttat ggttatacca gactataaag
     7021 atgtatacga ttttacacct atacagtatc cagcaaatga tacttcttgt ggtgttataa
     7081 ctactcactt tgattatcat tcaataagtg gtagaatact taaacttgat atacttggac
     7141 acgacggtcc taccattata aggatgcttg aagatatgac tggaataaat ataacagaaa
     7201 ttcctttaga tgataaagag actatgtctc tatttacatc tacagaagca ttaggtgtta
     7261 caccagaaga aataaactgt cctataggtt gccttgctgt accagagttt ggaactaagt
     7321 ttgtacgaca gatgttactt gacaccaaac ccactacatt tgcagaactt gtgcgtatat
     7381 ctggtctctc tcatgggaca gatgtttggg taaataatgc acaagactta gttagagaag
     7441 atatagttgg attaaaagat gttatatcta cacgagatga cataatgaac tatttaatat
     7501 ttaaaggatt acctcccaaa atgagtttta caataatgga gagcgtaaga aaaggtaaag
     7561 gtctaaaacc agaacacatt gaagaaatga aaaaaaataa tgttccagaa tggtatatag
     7621 gctcatgtaa aaagattaag tacatgttcc caaaagcaca tgctgttgct tatgtaatga
     7681 catcatttag aattgcatat tgtaaagttc actatccaga agcattttat gcgacttatt
     7741 ttacgacaaa agttgaggat tttgatgcag atttaattgt taaaggatta gatgctataa
     7801 aatcaaaaat aaatgaaatt gagtcactgg gaaatgatgc aaccacaaaa gaaaaaggta
     7861 tgttgaccgt tttagaagtt gctcttgaaa tgtatgctag aggaataaaa ttattaccag
     7921 ttgatatata taaatcagat gcaactgagt ttatagttgt aggagaaaaa acattacttc
     7981 caccaatggc agcaatacaa gggcttggag gaaatgcggc tataaacatc caaaatgaaa
     8041 gaaaaaatgg tgaatttata tcaaaagaag atttgagaaa gagaacaaaa atatcaaaaa
     8101 cggtaataga aactcttact aatcatggtt cacttgagaa tatgagtgag agaaatcaat
     8161 tgtcattgtt ttaagttagc ccaaaaagtt gcaaataaaa gaattttatg atatcatagt
     8221 atagaaaata gtatactttg cagaaactaa gagtgagaca gctgttctca ctcttttatt
     8281 ttaaatatat tattaaaaag ttggtttatt tttattaaat atattttatt taagattata
     8341 taaactaatc atttacaatt gaataggagg aactttaatg aaaaaaaatt tagaagctac
     8401 aattgaggag attgtaacta aaataacgga tgaacatgga tttgaaatgg ttgatgttga
     8461 atatgtaaaa gaagcaggag aatactactt aagagtatac atagataaag aagaaggaat
     8521 atctttaaat gaatgtgaat tagttagtag agaattaagt ccaatactag atgaaaaaga
     8581 ccctataaaa gaaaattact ttttagaggt atcttctcca ggacttgata gagcattaaa
     8641 aaaagacaga gattttgtta gatatcaagg tagagatgta gatttaaaat tatataaacc
     8701 attaaatgga tgtaagcaat ttgaaggtga acttgtagga ttaacagaag ataataatat
     8761 aaaaatcatt gcaaatggta aggaaatgga atttaataga aaagatgttg ctattgtcag
     8821 attagcaata aagttttagt ggtatattaa atagggagga tataaaaaat gaatcatgaa
     8881 tttatggaag cattagatga attagtagtt gacagaggta tagataaaga aatacttata
     8941 gatactatag aacaagctct tttaactgca tacaagaaaa actttggttc agcacaaaat
     9001 gttagagttg aatttgatag agacaaggga gatataaaag tattttctca aagagttgtt
     9061 gttgatgaat cagatttata tgatactttc ttagaaatag agttagcgga tgctagagag
     9121 ataaacccta attatgaatt aggtgatatt atagaacatg aagttacacc taaaaacttt
     9181 ggaagaattg cagctcaaac agcaaagcaa gtagttgttc aaagaataag ggaagctgag
     9241 agagaaatag tgtataatga atttatagaa aaagaaaatg aaatagttac aggagaggtt
     9301 gctagagcaa ataaaaatgt tgtttatgta aatcttggaa gaatagaagg tataatgact
     9361 caaagtgaac aaataccatt tgagaattat aaagcaggtc aaaaaataaa attctatata
     9421 cttgaagtta aaaagactaa caaaggacct caaatagttg tatcaagaag tcatccgggt
     9481 ctagtaaaga gattgtttga agaagaagtt cctgaaatat atgaaggaat agttcaaata
     9541 aaatctgtag caagagaagc agggtcaaga actaaaatgg cagtaaaatc tatagatgaa
     9601 aaaatagacc caattggagc ttgtgttgga ccaaaaggac ttagagtaaa aaatatagtg
     9661 gatgaacttg gcgatgagaa aatagatata ataaaatata gtgaagaccc agcagagttt
     9721 atatcagcag cacttagccc atcaaaagtt gttagagtag atatagatga agatgaaaag
     9781 tctgccttag taatcgttcc agattatcag ttatcgcttg ctataggtaa agaaggtcaa
     9841 aatgcaagac tagcagcaaa attgactaac tggaaaatag atattaagag cgaatctcaa
     9901 gcagaacaag aaatgttaaa tcttgaaaat tccaaagaaa tagaagctga ggatgttgaa
     9961 gctgaattta tcgaccaaga agagttaggt acttcagagg ataattttag tgaagaagag
    10021 taaggaggga tgattttgaa aaaagtaaaa aaagtgcctc aaagaaaatg catagcatgt
    10081 caagatagag actctaaaaa agagcttata agaatagtta aaaataaaga aggaaaaata
    10141 tttttagacc caacaggaag agccaatgga agaggagcat atgtatgtaa aagctctgaa
    10201 tgtctaaaaa aagctataaa gagtaaagca ttaaataaag cttttaaaat agatgttcca
    10261 gatgaagtct atgataatct acttatggag ttagaagaat atgaaaaata ataaagaaaa
    10321 agtactttcg ttcttagggc tcgcaactcg tgcagggaaa atagtgtctg gtgatgattc
    10381 aactttgctt gaccttaaaa aaggaaaagt aaatttgatt ttaatcgcag aagatgcatc
    10441 aaataatact aaaaaacttt ttaaagataa atcaactttt agaaatattc catatctatt
    10501 tttttcaaca aaagaggaaa taggatttgc aataggaaaa tctccaagag cagttgtagg
    10561 aataaaagat gaaaattttt ctaaaaagat aattgaacta atagaaattt aaaataaaat
    10621 agggggtgat cttgtgtcaa aaacaagagt ataccaaata gcagaagaat taaatatatc
    10681 aaatgaagag cttataaata agttagctga attagatata aatgtaactg acaaagattc
    10741 agtattagaa ggtgaagagt tagaattagc acttgaaatg ttaggtgaag atttatctca
    10801 agaaaatgga aatgttatag aaatagatgg aaagttgaca gtacaagtat tagcaacgaa
    10861 attagataaa tctccatcag aaataattat gaagcttatg aaaatgggaa ctatggcaac
    10921 aataaatcaa gagataagtt ttgagattgc tgctttggct gccaaagact atggatttga
    10981 attaacagta gctgaaagtg atgatacaga agctttagaa atagaagctt taatggaaat
    11041 tgaagaagat aaagaagagg atttaaaacc tagaccacca gttgttacag ttatgggaca
    11101 tgttgaccat ggtaagacat ctttactaga tgcaataaga aaaactgatg tgatttcagg
    11161 tgaagctgga ggaataactc agcatatagg tgcttcagaa gttaaaatca atggacacaa
    11221 aatagtattt ttagatactc caggacatga ggctttcaca tcaatgagag caagaggagc
    11281 acaagttaca gatatagcaa tattagtagt tgcagcagat gatggtataa tgcctcaaac
    11341 agttgaagct ataaaccatg ctaaagcagc aggtgtacct ttaatagttg ctattaataa
    11401 aatagataaa ccaggtgcaa acccagataa ggttaaacag gaattagcag accaaggatt
    11461 attagttgaa gactggggtg gagaagtaat agcagttcca gtatcagcta agaaaaaaga
    11521 aggaatagat acacttttag aaatggtatt attggttgct gaaatggaag agttaagagc
    11581 taatccaaat aaaagagcag taggaactgt tatagaagct gagcttgata aaggaagagg
    11641 gccagttgca acagtacttg ttcaaggtgg tactttaaca gtaggtgacc caatcgttgc
    11701 aggtgttgca tgtggtaaag ttagagcaat gataaatgca aaaggtaaga gagtaaaaac
    11761 agcaggacca tctacagcag ttgagatatt aggtctatca gaagttccac aaggaggaga
    11821 ccaatttgtc gaagttccaa ctgataagat tgctagaagt gtagcagcaa gaagacaaca
    11881 aatagttaga gatgaaatgt taaaatcaac tcaaagatta tcactagatg ctctatttag
    11941 tcaaatgagt gaaggttcta taaaagacct taatatagtt ataaaagcag atgtacaagg
    12001 ttcagtacag gcagttaaac aatctttaga aaaattatca aatgaagaag ttcaagttaa
    12061 agtaatacat ggtggtgttg gagctgtaac agaatcagac atattacttg cagcagcttc
    12121 taacgcaata ataataggat tcaatgtaag accagttcca ggtgcagaat ctctaggaga
    12181 aaaagaaaat gttgatataa gaacatatac tataatatac aaggcaatag aagatataca
    12241 agcggctatg actggtatgt tagaccctga atatgttgat gaagaaacag gaaaagctga
    12301 aataagagaa atatataaaa tatctggtgt tggaacagta gctggatgtt atgtgactaa
    12361 tggtaagata tttagaaatt gtaaagtaag attagttaga gatagcataa taatacatga
    12421 aggtgagctt gcagcactta agagatttaa agatgatgta aaagaagtta attctggata
    12481 cgaatgtggt atgagttttg taaattataa cgacataaaa gaaggcgata tagtagaagc
    12541 ttatataact aaagaagtag aaagaaaatt ataaattcgt atcaaagaaa atcagttagt
    12601 aaagaaggtc gatggtatgg catcatataa tagaacaaga aggatagcag aagaaataag
    12661 aaaagtcgta agtactatgc ttataaatgg agtaaaagac cctagaataa catctatggt
    12721 gagtgtaact gatgtagaag ttacaaatga cctaagatat gcttatgtat atgtaagtat
    12781 tctaggtgga gatgaagaat ccacactaac aggtcttaaa agtgctggag gatatataag
    12841 aagagaagta ggaaaaaata taaaacttag atatattcct gagatagtat ttaaacttga
    12901 tgattcaata gaaaaaggaa tgtatatgga taaccttata aaaagagtta atgaaaaaaa
    12961 tgctcaacaa aatgaggatg aaaattatga tgaataatat ggatagtatt ataaactata
    13021 tttatgaggg tgactatttt atagttacct ctcatatcag tccagatgga gacaatgttg
    13081 gttctacact ttcaatgtat tatacattga agaatttagg aaaaaatgta tattatgtac
    13141 ttgatgatga agctccatta aatttaaggt ttttggttga aggtgtaaaa atctataagt
    13201 ctaatgaatt taatatgaag aactattctt taattgcact tgattgtggg gataagatga
    13261 gaatatgtgt gagtgatgaa atcaaaaata atgcatcaaa attaatctgt attgaccatc
    13321 atgctagtaa tgattcatat ggagatttaa attatgtaga tattgatgca tcttctacat
    13381 gtgaactagt ttataattta ttggttagat ttcagcaaac aaaagatatt aatattataa
    13441 atgaagatat agcaacatgt ttatatacag gtcttgtaac tgatactgga aacttttcgt
    13501 attcaaatgt acatgcaagt agttttgaaa tggcaaaaaa tttacttgtt ttaggtgcac
    13561 aaaaaaatac tataatacaa aatatatatc aaagcaattc ttctgattat tataaattat
    13621 taggagaagc tctaaaaggt ttggagatat ttgattcaaa ggtatctagt atagttttaa
    13681 ctcaagacat gatgaataga aataatatta gctttaatga tgttgatgga ataacaacat
    13741 atacaagaga tataaaaggt atagaagttg gaattttatt taaagagaaa aaacaaaatg
    13801 aaattaaagt gagttttagg tcaaaaaatt atgttgatgt aagtgaaata gccaagttat
    13861 ttggcggtgg tggtcatgta agggctgctg gatgtactat tagagatagt attgataatg
    13921 caaagaaaat ggtactagag gcagtgttaa aatcaattta aggtgacagt aaaatgaata
    13981 agattataag tatattgaaa cccacaggta tgacatctca tgatgttgta agtagagtga
    14041 gaaagatttt aaatataaaa aaagttggtc atacaggtac tcttgaccca gatgcttcag
    14101 gtgttttacc aatctgtata gggaaagcta caaaagtatg tgaagttatt ttaaacaaag
    14161 ataaatcgta tatatgtgag cttactttag gtataagtac agatacttat gatgcttcag
    14221 gtgaaatttt aaaaaaagtt gatgatttta aatttagtaa tgaagacata gaaagagctt
    14281 ttgatacaca aagaggagag ataaatcaac ttcctccaat ttattcagct ttaaaggtaa
    14341 atggaaagag aatgtgtgat ttagtaagaa gtggtagaca atctgaaatt actttaaaaa
    14401 caagaagagt aaatataaaa gacataaaaa tactaagtat aaaaggtaat aaagtaatgt
    14461 tttatgttga gtgttctaaa gggacttatg tgagaagtat ttgccatgat ataggagaat
    14521 atctgggttg tggagctcat atgtcgtttt tgaatagaac atcttctggt aaatttgact
    14581 tagataattc aataacatta gaagaattag aattatttta tgaaaataaa actttagaca
    14641 aatacttata tgatatagat tatgtccttg attcgtttaa ttatgttgta ttaaacccta
    14701 atgctataaa atattatagt aatggtggct caatagatga taaaaggttt ttaaaaaata
    14761 atttcgataa agatgatgaa gttgtaagag tatacagtac agataatttc ttaggattag
    14821 gaaagttatc aaaacacaat aatacaataa gtgttaaaag tgataagatg ttcatataac
    14881 caatgtattt aaaaaaacaa caaaagagat agtactataa taacttctat agtctatctc
    14941 ttttacttat gctataatag tttgtgaata agttaagagt aaattatatt ttagcgggga
    15001 acatttatta tggtgattat aaaatctata aaagaaatag caaacataaa agaaagtgtc
    15061 attaccatag gaaattttga tggtgtacac aaaggacatc aagttcttat aggaaaaaca
    15121 gttgaatatg caaaaaaaga aaatatacag agtattgtat ttacatttgc aaatcatcct
    15181 gtaaattact ttagaccaaa ctcaacaaaa aatattataa gcaatgatga taaaataaaa
    15241 ttattagaag atttgggaat agatatagtt gtaatcatac catttgatga gtatatgact
    15301 aaaatacctg caaaggattt cgtagatgag atactagtaa aaaaattaaa agctaaaaag
    15361 atagtaattg gacatgactt tacatttgca agggcaaaag aaggcaatgt taatctatta
    15421 aaaagcttag aacatgaatt tggttttgag gtagaagtaa taaaacccat caaaatcaat
    15481 gatgtaagag tgagtagtac ttatattaga agtttagtat cccagggtga tatggctaat
    15541 gtcaaggaat atttgggtag aaattacaag ttagaaggtt gtgttataca ttctaaacac
    15601 cttggaagaa ctataggatt cccaacggca aatattgacc ttaagaataa tatgctcgtt
    15661 ccaaagagag gtatttatgc ttctattgta catattggag atgagactta ttttggtgct
    15721 acaaatgtag gctataaccc aacagttaat ggaaaaactt tgtctattga aacaaatatt
    15781 ttagagtttg atagagatat ttatggggaa aatataattg ttgaattttt ggaaagaata
    15841 agagatgaaa gaaagtttaa ttcaatagat gacttaaaaa atcaattgta taatgataca
    15901 aattatgtgt atgaaaacta tgtttgcaaa aacaagtaat gtatgataaa atgattaagg
    15961 aaaataacca ttactcggca tttgaatact ccaatcaatg cttagtaata ggcgtttcta
    16021 aaaaaatacg gaggtaatct aaatgataaa caaatcagaa ataataaaag aatatggaag
    16081 agctgaaggg gatactggtt ctccagaagt acaaatagct ttattaacag ctagaataaa
    16141 cgagttaaat ggacatttaa aaactcataa gaaagaccac cattcaagaa gaggactatt
    16201 aaaaatggtt ggtagaagaa gaaacttatt agcttactta aaggaaaaag atcttgaagg
    16261 atatagagca ttaatagcta aattaggatt aagaaagtaa tttcattctt tataaaaaag
    16321 caggtatagt gtatacctgc ttttttatag ttaattcatt ataaagtaaa atagttaata
    16381 ataaaagtag tttatatgtt ataaaaaatt ataaaatgaa taatattggg taagaatatt
    16441 aaaatagtat acaaatacat aatcaattta aaaggaaaga caggataaat atagaaattt
    16501 tataacagga taattagttg gaataaggag gatattattt tgaaaataaa gttagtatgc
    16561 gatagtttgt gcgatattcc agatgaaata tcagaaaaag attatataga gatagttcca
    16621 cttacagtta tttttaatga tagggaatat atagaaggag tagatataaa gaaagaagag
    16681 ttttataaaa aagtaaaaga aatcaagcaa atacctaaaa cttcacaagc tacatatatg
    16741 gagtttaaag aagtgtttga taagttcata acagaaggtt atcatataat ctgtatgaca
    16801 ggggctgcaa acgcatcagg aacttttcaa agtgctatga tagctaaaaa tgatgtaaat
    16861 gaaaatgaaa aaatacatat atttgatact agaaatttat ctctaggtag tggacaatat
    16921 gttataaagg catgtgaact tcttgaagaa ggactgggat ttgaagaaat tatagatgaa
    16981 ttagaaaata ctcgcagtag tgtaagatta ctttttgcac catatacact tgatttttta
    17041 aagcagagtg gaagagttcc agttgcaact gctttgatag gaaatatgtt aaatataaag
    17101 cctatatttt tctttgataa tggggaagct aagttggtaa ataaggttag aggaataaag
    17161 aatatagcaa gtaaactggt cgatataatt ttagaaatga atgaagggtc tttagaagga
    17221 aaaatcgtca caataggttg tggagataat ttacacgatt gtgaaatatt aaaagatgaa
    17281 gtaaataaaa gaataaaagc aagaagagta ttatttactc gaggaggagt ttctatttgc
    17341 tctcatacag ggccagatat attggctata agttgttcta attagtagta atataaggtt
    17401 tttgcatata tatgtgaaga taattttata ttgttaattt atagtatttt tgataatata
    17461 gtaaatagag tgatatacgt gttttttaat aagaggagga caagcattaa tgtttgaaca
    17521 taaaatattt aaaatggatt ttgcaggaag agaactttct gtagaaatag gaaagatttg
    17581 tgaaatggct agtggtagtt gtatagttcg atacagtgac agtatggtaa tggtaaatac
    17641 aactaaatca gccaaaccta gagacggaat agactttttt cctttaagtg tggattatga
    17701 agaaaaatta tactctgtag gtaagatacc tggaggtttt ttaaagagag aagggaagcc
    17761 ttcagaaaaa gctatactta catcaagatt gatagataga ccaataagac ctttattccc
    17821 aaaaggattt agaaatgatg ttcaggtagt tgcaacagtt ctatcagtag accaagattg
    17881 tacaccagat atagtagcaa tgataggttc ttcaatagcc ctttctatat cagacatacc
    17941 atttaatgga ccaacaggtt cagtatgtgt tggccttgta gatggtgcat ttgttgtaaa
    18001 tccaaacgca gagcaaagag aaaaaagttc aatgcatcta gttgtttcag gtacaaagga
    18061 agcaataatg atggttgaag ccggagctga tgaagttcca gatgaggtaa tgcttgatgc
    18121 tattttattt gctcaccaag aaataaaaaa aatagttgag tttatagaag gtatagttgc
    18181 agaagttgga aaagaaaaaa tgcctgttga attataccat gcaggagaag aaataactca
    18241 attggttcgc gaatttgcaa ctgataagat gaaaaaagca gttcagactt ttgaaaagtt
    18301 agagagaatg gaaaatatgg atagagttaa agaagaaact ttagctcact ttgaagaaac
    18361 tttagaagat tttgaagatt ttgttggaga tatagaagaa gtattacaag atataataaa
    18421 agaagaagta agaaaactta tagtacatga aaatgtaaga ccagataata gaaaacttga
    18481 agaaataaga cctatatggt gtgaaactgg catgatacct agagctcatg gttcagccat
    18541 atttactaga ggacaaactc aagtacttaa tgttgctact ttaggtgctt taggagatgt
    18601 tcaaaaatta gatgggttag atgaagaaga aaacaagaga tatatgcatc attataattt
    18661 ccccgcatat agtgttggag aagctagacc atcaagagga ccaggaagaa gagaaatagg
    18721 acatggtgct ttagcagaaa gagctttatt accagtaata ccatctcaag aagaatttcc
    18781 ttatgctatt agattagttt ctgaagtatt aagttcaaat ggttctacat ctcaagcaag
    18841 tgtatgtggc tcaacattat ctttacttga tgcaggtgta cctataaaag atatggttgc
    18901 aggtatagca atgggactta taaagcatga tggtaaggtt gcagtgcttt cagatataca
    18961 aggtatggaa gaccatttag gcgatatgga ctttaaagta gctggaacag agtatggtat
    19021 aacagcaata caaatggata taaaaattga tggtatagat aaagaaattc tacaacgtgc
    19081 tttaaaacaa gctaaagagg gtagaatcca tatactagga gagatgagaa aaactatatc
    19141 tcagccaaaa ccagaacttt caccatatgc acctaagatt gttaaaatgc aaataaatcc
    19201 tgacaagata aaagatgtaa tagggcctgg tggaaagata ataactaaga taatagacga
    19261 aactggagtt aagatagata ttgaacaaac tggggaagta tttatatcag gtattgagat
    19321 tgatatgatt aagaaagctc aagaacttat aaataatata gtagttgaac cagaagtagg
    19381 aaaaacttat aaaggtaagg taagtagaat aatgaacttt ggagcatttg ttgaaatact
    19441 tccaggaaaa gaaggattac ttcatatatc tcatatagct catgaaagag ttgccaaagt
    19501 tgaagatgta ttgaatatag gggatgaagt tgaagttaaa gttacagaga ttgatgaaaa
    19561 aggaagagtt aatttatcaa gaaaagttct tttaccaaaa ccagaacata aaaataaata
    19621 acttattata taattaaaag caaagtctta ggattttgct tttttaaata caaaaaagta
    19681 gataatttta aaatgatagt tatggatggc ttatagttct ttttctgtaa ggctttactc
    19741 ttataaagaa gctatttaaa agctgattaa tagaaagctt tattgatata tatagaatca
    19801 cttaagttat atttagtgca taaacagagc ttatttttaa tagtattaaa taagaaacaa
    19861 tttgtacata attaggggga gttttaatga taatgatggt acttaataag agtaagctca
    19921 aaaaaatcat aatactaatt ttagtagtgt tattagtatt atcaggaggg gtaatggtat
    19981 taaagttatt taacaacaag ccagtattta atttatttaa tggtgtagat ttaccatatg
    20041 aaagaggtac aaaggataaa agtggatatg ttgcattaac ctgtaatata gaccttggat
    20101 gggaaacaga gtacgtagaa tccattttag aaacacttaa aaaagaaaat gtaaagatta
    20161 catttaatgt aactggaaaa tgggcagaaa agaataagga tgaattatta aagataaaaa
    20221 aacaaggaca tgaaataggt aatcatggat ataaacattt agattattct acattatcat
    20281 atgaagacaa ttatgagcaa atagagacct ctaaaaagat aattgaagaa attataggtg
    20341 aaaaaactaa attttttcaa gcaccagcag gttcctttgg tccagaaaca gtaaaggctg
    20401 ctaaagcatt agggtatact tctatcaaat gggatgccga tactatagat tggaaataca
    20461 aagaccaacc agaggtaatc attgatagaa tgaagaaaaa agacattaaa gatagtagta
    20521 taatcttaat gcatccaacc aacgcaacga caaaatgtat agatgatatt atagctattg
    20581 taagagaaaa aggactaaaa ccagggaaac ttagtgatgt atttaagtag acaaagtata
    20641 aatatttggt taagattgta taagttgtag ttgaattaat taaaagactg acttataatg
    20701 taatacatat aaaaaataaa tataattaaa acatagtgtt ttcaataatc caatatgaaa
    20761 caaagaataa attgttagga gaatgtaatg tataagacaa aaatactgga aaatgggctt
    20821 acaataattg gtgaagaaat accatatcta aagtcaataa cattaggtat ttggataaat
    20881 gcaggctcta ggatagaaga agcacaggtg agtggaactt ctcattttat agagcatatg
    20941 atgtttaaag gaaccaaaaa cagaacatca aaagagattg ctagttcaat agataatttg
    21001 ggtggtcaaa taaatgcatt tacaagtaaa gaatgtacat gttattatgt aaaactaata
    21061 gatgagcata tagatacggg aattgatgta ttgagtgata tgatactaaa ttctaaattt
    21121 gataaaaatg atatagataa ggaaagactt ataatattgg aagaacttaa gatgtatgaa
    21181 gactctccag atgacttatc ttatgattta ttagtggaaa atatatatgc taatgatgga
    21241 ttaggaatga atataattgg gacaaaagaa tctctataca atataactag agaatccatg
    21301 ttagaatact taaataagta ttatattcca aataacgctg ttatttctat agctggaaac
    21361 tttaactttg atgatatggt ggagaaaatc aaatcaaaat ttggacactg ggaaaagaaa
    21421 aatttgagta tagacataag tgaggctaag tttaatcctt gctttatatc aaaaaataaa
    21481 gatactgaac aggtgaactt agctatctgc ctaaaaggta ttccttttga aaatgatgaa
    21541 gaagtctact caatggcagt ggtaaataat attttcggtg gaagtataag ctcaaggctt
    21601 tttcagaaaa taagggaaga aaaaggttta gtatattcaa tatactcatc acagacatta
    21661 tataggaaat gtggtgaatt aggcatattt gcaagtatga gtacagaaaa tttacaagat
    21721 gtatataatt taataaagaa agaaattgaa aatataagag aaaactatct tacagaaaaa
    21781 gaaatatctg aaagcaagga acagttaaaa ggaaattata tattggattt agaaagtact
    21841 agcagtagaa tgatgtctac tggtaaatct atgttactta gcaaaaaagt aaaaactaca
    21901 gatgaaatac ttgaatgtat aaataatgtt aatattaatt caataaagaa ggttgttgat
    21961 aaggtattca atatcgaaaa tattggtaca tgtatagttg gaagagatgt agaaaagatt
    22021 ctacacttgg attaatattg aaaaaagtaa atctctggag gtataaaata tgaatttatc
    22081 tgaaatagca ggtaaggaaa tagttaactt ggtcacagga gaaagattgg gggtcattgg
    22141 agaatgtgac cttatactag atgaaactac aggcggtata ttagcgcttc taattccaaa
    22201 ggaaaaagga ttttttgcca gaagaaaaga taagtcattt ttagaagtac catggagaaa
    22261 tgtaaaaaaa ataggtaatg acatgataat tatagaatat gagggatata tttaggagga
    22321 aaaaatgagt attttagtgc aaaagtttgg tgggacatca gtagaatcat atgaaaaaat
    22381 gaatgaagta tgtaagatag taaaagcata caaaaaaaat gatgaagaat tacaattagt
    22441 attagtagtt tcagcaatgg gaagaaaagg agctccatat gctacagata cacttataaa
    22501 tttgtgtagt gcagtaaatg atgagccaag taaaagagaa cttgatttaa taatgtcttg
    22561 tggagaaata atatcaggaa caattcttgc taatttattg aatgcacagg ggatagattc
    22621 tgttttcttt actggtcagc aagcaggtat aattacaagt gatgaatatt ctaatgcaaa
    22681 gataaagtat ataaatccta aaaaggtaaa aagagcttta gatgatggta aggttgtagt
    22741 tatagctgga ttccagggtg taacagatga tggagaaata actactcttg gaagaggagg
    22801 aagcgataca tcagcagttg ctattggtaa agctttagaa tgtgaaacag ttgaaattta
    22861 cactgatgtg gatggtataa tgactgcaga ccctagagta gaaccaaacg ctaaagtatt
    22921 gagttttata gattatgagg aagtattcca aatggctgac aaaggtgcaa aagtaataca
    22981 tccaagagca gtacagttgg ctaaaagtgg aaatataact ttagccataa agaatacaat
    23041 gaatccaact tttgaaggta caaaaatagg ttctttatgt agacacttag aagagaatat
    23101 agaatatgaa caagaaagag attttaaggt tgctgttgca aataaagata gtgtagcaca
    23161 agttaagatt aaatcagcag aagaagtgtt tacagaagta ttaaatgaga tagaaaagaa
    23221 acttataact atagatatga taaatttctt tatatcagaa aaagcatttg ttgtagaaga
    23281 tgctgatata aaatcattaa aggaaatact agataaattt gaattagatt atgaagtaaa
    23341 tagagactgt gctaaggtaa cattgatatg ttcaagaatt gctgatgaaa tgtctggtat
    23401 catgtcaaag gtagttagag gattatcaaa agcaggggta tctcttttac aaacatcaga
    23461 ctcaaatatg actatatctt gtttagttag tgaagaagat atgcatacag cagtacatgc
    23521 tatacaccaa caattttatt taaaataaac tgtataatac aattattttt taatcaaatt
    23581 taagaatttc atatatatct taatatattt gatttatttg gtaaaactaa attaaatata
    23641 attttgatag gagttttttt atgaaagatt atagagaaga taatgaaaga tttgataaag
    23701 ataattgtag tgaagaatct agttgcgaag agagagaatg tataaagcaa tttggtacca
    23761 atgaaatgcc acctcaacct cctaaagata ttcaatgtat aactataatt ggagaaattg
    23821 agggacattt tataggaaat cctcaaaaaa aagctacaaa gtacgagcat ataattccaa
    23881 tgctatactc aattgaggaa agcaatgatg ttaaaggagt gttagttgtt ttaaacactg
    23941 ttggaggaga tattgaagca ggtttagcta ttgcagaact tttaaacagt acatctaaaa
    24001 aagtagttac tcttgttcta ggaggaagtc atagtatagg tgttcctctt gctacagcag
    24061 gagattactc atttatagca ccaacagcaa ccatgattat acatccagtg cgaactactg
    24121 gtcttgtgat tggtataaat gagacttttg aatattttaa aaagatgcaa gatagaataa
    24181 ttcaatttat aataagaaca tcaaatatca aaaaagatgt acttgaaaaa cttatgcatg
    24241 aaaaagatga acttgtaagt gatgttggta gtgtattaat aggaaaagaa gctgtggatt
    24301 atggtcttat agatgaagtt ggaggactaa aagaagcact taaaaaatta agagagctta
    24361 taaaagaatc tgaggaagaa aaaaataatg attaaaattt gtttaaataa taaaatttat
    24421 gatataatga tgtaataaag taggagtgtt agtgttaagg cattagcact ttttgctgtg
    24481 aaggggtaaa acttgctaaa aataaggagt gatattaatg gccacgaaaa agaagaaaaa
    24541 aaagaaggta aataccttaa gttttaatgc agaatatcat aatttaataa caatatttat
    24601 aggtgtattt ttattatata gtttaaattc taattctatg ggatggatac ctgtgttaat
    24661 gcaaaatctt tttaagggac tatttggagg gctttcaata gcaataccat ttatagtaat
    24721 tataacagga cttttaggat tttttgatgg aaatgaatat atatataggc taagaaagac
    24781 taaactatac tacataatta ttttatttat atttgtattt tatggattat taaatgcagg
    24841 aactcttcct gtagatagcc cactaaaagg taacatgttt gatgatgtta tgaaattggg
    24901 tgtaagtgga caaggtagtg gtttgatagc aacaactatt gcgtattata tgagtaaaat
    24961 atttggtata gcaggtggat ggttaataag tatcttcgca cttatacttt ctgtaatgtt
    25021 tatatttaat atttctataa aagatttggt gtcaaatgca aaatcaaaat ctaaagcatc
    25081 taaagacagc aatttaacgt ttaaagataa aattgcaaat atgaaaaaat ctgcaataga
    25141 tatgatgact gatgaagtag atgatactac tataaataaa aaaccaggtt tctttaaagg
    25201 gttaatgtca aaaggtctta ataatgatga tgaagaagat gaatattttg aagaagaaaa
    25261 tagtgatggt gtagatgata aaactataaa aatagttgga tttaataagg cagaagatga
    25321 gtatttagaa atattagaag gaactcaaag tatgccagag ttagatgttt taaaagaatt
    25381 acaaaaagct accaatgaaa atccagttgt agatactaaa ccagaaaaaa aagttgatat
    25441 agctaaatct aatttaaata tagaaaaaac tcaacctatg agtattgtag cagagccagt
    25501 taatgaggat tattcaaact ataaaaagcc tagcattgag cttttaaata aagtaaataa
    25561 gaagtctgat gaaaatggta aaaagaaagt gttgaaaaat gcttctcttt tagaaaagac
    25621 actatcagat tttggagtgg aagcaaaaat taatcaagtt acagttggac ctactataac
    25681 aagatatgaa atacaaccaa gtccaggagt caaggtaagt aaaattgtaa atttaactga
    25741 tgatatagct ttaagtttag cagctaaaag tataagaatt gaagccccaa ttcctggtaa
    25801 aagtgcaatt ggaatagaag ttccaaatga agaagctcaa atggttggtg tgagagaagt
    25861 tctagaaagt gaagaattta ataattttaa ctctcctctt gcaatgggac ttggaaaaga
    25921 cgtagcagga aaaattataa ttggagatat aggtaagatg ccacacttac taatagctgg
    25981 ttctacaggt tcaggaaaga gtgtgtgtgt aaatacatta ataagtagta ttttatataa
    26041 ggcaaatcct gatgaagtaa aattactatt aatagaccca aaagtagttg aacttgcaaa
    26101 ttataatgga ataccacatc tattgatacc agtagttact gaccctaaaa aagctgcaaa
    26161 tgctttaaat tgggcagtta cagagatgaa tagaaggtat aaattatttg cagatgctca
    26221 agtaaaagat gtaacaagtt ataatgagaa agcggaagaa aaacttccta aaatagtaat
    26281 aataatagat gagttagcag atttaatgat ggcaagtgca aatgatgtag aagattatat
    26341 ttgtagatta gctcaaatgg caagagctgc tggaatgcac ttgattgtag caactcaaag
    26401 accatctgtt gacgttataa caggggtaat aaaggctaat ataccatcta gaatagcatt
    26461 tgcagtatca tctcaaactg attcaagaac aatccttgat atgggtggtg cagagaagct
    26521 tctaggtaaa ggtgatatgt tattctatcc acttggtgca gcaaaaccag ttagacttca
    26581 aggggcattc atatcagaga gtgagtcgga aaaagtaatt gattttgtaa aaagtcaggt
    26641 taaagatgga ataaaatatg aagaagatat aattgaaact atatctaagg ttaatacttc
    26701 aaagagtagt gatgaggatg agtttttgag tgaagcgata gaatttgtcg tggaaagtgg
    26761 tcaagcatct gcctctatgc tacagagaag atttaaaata ggattcaata gagcagctag
    26821 acttatagat tctatggaag aaagaggcat aattggagca agtgagggaa gtaaacctag
    26881 aaaggtatta ataagcaaac aggatttgca gaatttagaa ggtgaataga attgtcagtt
    26941 ataaaaatag aaaaagcact taagctagat aaagtaatat ttgtagatgt gagaactgaa
    27001 ggagaatatg aagaagacca tatattgaat gcaatcaata tgcctctttt taaaaataat
    27061 gaacataatg aagttggaac aatttataag atgcagggaa aacatgaagc aatacaaaaa
    27121 gggtttgact atgtatcgta taaattaaag gatatatatt tacaagcagc agaacttgca
    27181 ttaaattatg ataatattgt catatattgt gcaagaggcg gaatgagaag tggaagtata
    27241 gtaaatttat tatcatcatt aggtgtaaat gtatatcaac ttgaaggtgg atataaggct
    27301 tatagaaatt ttgtattaga gtatctaagg catgtaatgg atacaaaaaa ctttgtatct
    27361 gttcatggat tgactggtac aggaaagact gatttgttac atttattaga tgaaaaagga
    27421 atagatgtac tggatttaga aggcatagct aaaaacagtg gttctacctt tggttttata
    27481 acttttgata aaaagccacc atctcaaaag aactttgaaa caaaactatt tgaaagtgta
    27541 tttttttcga aagagaatta tatatttgtc gagagtgaaa gcaaacgagt tggaagtgtt
    27601 acagttccaa atgaaatttt cgaggcaatg acacgagaag gacatcatat attattagaa
    27661 tgtgatattg aaaatagagt tgatagactt tgtagagatt atatttatga taaagatgaa
    27721 ggtaatatat tgattttaaa agaatgtata aacaagttta gaaaaagatt gggtaataaa
    27781 gtagtagatg attatattga tttattagaa tcaggtgaat ataaagaact tgtaaaaaga
    27841 tatcttttag aatactacga cccactttat atgcattcag tagagaaata taagtatagt
    27901 aagattataa attttaatga tacacaaaaa gctttagaag atgttataga cttatatgag
    27961 agtatattgg aaggagaaag tgaatgttaa aaatagcatt ggaatcatta ggatgctcaa
    28021 aaaatttagt agatgcagaa ataatgatgg gaattttaaa taacaaaggc tataaattaa
    28081 ttggagattt tgaagaagct gatgtaatta tagtaaatac atgtgggttt atagagtcag
    28141 ctaaacaaga atctatagat acgataataa acttttcaga gttaaaaaaa actggcaatt
    28201 taaagttgtt gatagtaaca gggtgtctag cccaaagata ttctgaagaa ttaaaaactg
    28261 aaattcctga aatagatgct atagtaggaa caggaagcta tcaaaatata gataaaatac
    28321 ttaaagaatt aagtgaaatg catcaaattg taagccttaa tgatatagag tttgtattta
    28381 atgaagattt accaagatat atatctacac ctagctatat ggcctattta aaaattggag
    28441 aaggatgctc caataattgt acttattgta taataccaaa actaagaggt aaatatagaa
    28501 gtagaaaatt tgaagatata attaaagaag ctaagaaatt agctgaatct ggagtaaaag
    28561 aacttgtggt tatagcacaa gatacgacaa agtatggttt tgatttatat ggaaaagaaa
    28621 gattatcaga acttttagaa gaacttgcaa aaatagatgg atttaagtgg ataagggtta
    28681 tgtattcata tccagaatct ataacagagg aattgataca agttataaag aaatatgata
    28741 atatatgtag ttactttgat atgccaatac agcatgcaag taacaatatt ttaaaattaa
    28801 tgaatagaaa aactactaaa gaagatatat taaataaaat caatctgata agaagtaata
    28861 taccagatgc tatacttaga actacgataa tagtagggtt tcctggtgaa acagaagatg
    28921 acttcaaaca acttgtggac tttgttgaag aggttaaatt tgatagactt ggtgcatttg
    28981 catattcaag agaagaagat acaccagcag atagattgcc aaaccatata gatgaagaag
    29041 taaaaataca aagaagagat actttaatga tgattcaaca aaaaatatct gaagaactta
    29101 atgacaaaaa gataggaaaa acttatgagg tgttaataga agaacagata gaagataatg
    29161 tatatacagg aagaacacaa ggtgatgcgg aagaaattga cagtatagtg tatgtaaaaa
    29221 gtgtagacaa tcttgaagta ggagaatttg taagtgttca aataaatgat gcaatggaat
    29281 acgatttaat gggagatgtt ttatatgaac ttgcctaaca agttaacttt gtttagaata
    29341 tttttaatac cagtatttgt tttaattatg ttgctcaatg taccaaataa atttttaata
    29401 gcttgtatta tttttataat agcttcaatt actgatgctt tagatggaaa aatagctaga
    29461 aaatacaatc tagtcactga ctttggaaaa tttatggacc cattagcaga taagttattg
    29521 gttatttcag cacttacatg tatgatagaa gaccatcttg taagtagttg gatggtaatt
    29581 ataatagtgg ctagagagct tacagttagc atattaagag ctatagctgc tgctgatgga
    29641 aaagtaattg ctgctggaaa cagtggaaaa cttaaaacga taactcaaat ggtatctata
    29701 gtgtttttat tgttaggcgc tcagtttgaa aatgtattga tattaaatat tggtgaaatt
    29761 ctcattctta tagctacact acttacatta tattctggat gggaatatct atataaaaat
    29821 aaagagcttt ttatgtcttc taagtaaaaa attatgcttt aataaataaa aactaatgat
    29881 taataaataa aaaataacaa cttaaaattt taaaaatgta aaaaagggta tactattata
    29941 gtatacctct tttcatttta agaatatttt cttttgtgta tataattatt aataatggtt
    30001 tataaataaa atgtaatgaa taaaaatata atcattagta gcgtttttaa attgactagg
    30061 tttgatttat gttatataat taatatataa caagagaaca aatgtttgta gataaatgaa
    30121 gggatggtat agagatgagt gtagatcaag aaaaattaaa agcgttgaat gaagctttag
    30181 gtaaaattga aaaagatttt ggtaaaggtt cagtaatgaa attgggagaa gcaacatcta
    30241 tgtctataga tgttatatca acaggagcga ttggtttaga catagctatt ggtataggag
    30301 gtctacctag agggagaata gttgaagtat atggtccaga atcttctggt aagactactg
    30361 ttgcgcttag ttgtgtagca tcagctcaaa aagatggagg aatagctgca tttatagatg
    30421 cagaacatgc acttgaccca gtatatgcaa aagctttggg tgtggatgtt gataacctaa
    30481 taatatctca accagataca ggtgaacagg ctttagagat agcagaggca ttgataagaa
    30541 gtggagcgat agatataata gtaatagact cagtagcagc attagttcca aaggctgaaa
    30601 tagatggaga tatgggtgat tctcatgtag gtttacaagc tagacttatg tcacaagcac
    30661 ttagaaagtt aactggttca attaaaaaat caaattgtgt tgctatattt ataaaccagt
    30721 taagagagaa agtaggaata atgtttggaa acccagaaac tactactgga ggacgtgcac
    30781 taaaattcta ttcatcagtt agattggatg ttagaaaaat agatacaata aaacaaggtg
    30841 ataaagttat aggtagtaga actagagtta aagttgttaa aaacaaagta gcaccaccat
    30901 ttaagcaagc tgaatttgat ataatgtatg gagaagggat ttcaaaaatt ggagaccttt
    30961 tagatatagc tgctgatgta gatatagtta aaaaatcagg ttcatggtat agttacaatg
    31021 atactaaact tggacaagga agagaaaatg ttaaaaaatt cttggaagat aatttagatt
    31081 taactactga aatagatgag aaagttagag cattttacaa tttaaatgaa gaacatgaag
    31141 aatcaggtac ttcagtatca aaagaaattg tagaagaata agtaaaaata caaaaaaata
    31201 actagtaatt aaagacatgc cacgctccct attagcttga cacaatgttg aaaaaattat
    31261 aaaattaagt gaggataaaa tatctaaatt taattttaat tattaggaat attttttaat
    31321 catagtgatg acattttggg attattaacg aaggaggtgg atgtcataga tagtgtagta
    31381 atgattgtaa taggtgcagc tgttggtatt atagctggat attttgttag aaaaaatata
    31441 tctgaagcaa aaattggaca agctgaaaac ttggccaaag aaataataga taaagcacac
    31501 cacgactcag aaacagtaca aaaagaaaaa ttactagaag ctaaagaaga gatccataag
    31561 tggagaacag aagctgaaag agaaaacaga gaaagaagaa cagaagtaca aaagtatgaa
    31621 aaaagagtag tacaaaaaga agaagtctta gatagaaaat tacaaaattt agaatctaaa
    31681 gaagtaaacc taagtgaaaa attaaaggta gtggaaaaga aagaagaaga agtcgaagta
    31741 ataaaaactc aacagttaga aaaattagaa agtatatcag gaattacttc tgataaggcc
    31801 aaagaaatca tcttaaccaa tgcggagaga gatgtaagac gtgaaatgtc tataatgata
    31861 aaagagatag aaagtcaagc taaagaagaa gctgataaga aatcaagaga aattatagga
    31921 tatgcaattc aaaaatgtgc agcagatcac gtagccgaaa ctactgtaac agtagtaagc
    31981 ttgcctaatg atgaaatgaa aggtagaata ataggtaggg aaggtagaaa tataaggact
    32041 ttagaaacct taacaggtat agaccttata atagatgaca ctccagaagc cgtaatttta
    32101 tcaggatttg atccaataag aagagaaatt gcaagaattg ctctagaaaa attaatagca
    32161 gatggaagaa tacatccagc tagaattgaa gaaatggttg aaaaggcgag aaaagaagtt
    32221 gacaatatta taaaagaata tggtgaacaa gctgcatttg aaactagtgt acatgggatt
    32281 catccggaat taataagatt attaggtaga cttaactata gaactagtta tggacaaaat
    32341 gtacttaaac attctataga agttgctcat atagctggta taatggctgc tgaaatagga
    32401 gcagatatta gattggctaa gagggcaggt ttattacatg atataggtaa ggctgtagac
    32461 catgaaatgg aaggtacaca tgttgaaata ggtatggatt tacttagacg ttataaagag
    32521 tctaaagaag ttatacatgc catgagtact catcatggag attacgaacc tcaaacagta
    32581 gaggctgtat tagttacagc agcagatgca atatcagctg ctagacctgg tgcgagaaga
    32641 gaaactttag aagcatatat aaagagactt gaaaaattag aagaaatagc taattcatat
    32701 gaaggtgtag aaaaatcatt tgctatacaa gctggtagag aaataagaat aatggtaaaa
    32761 ccagaagcta taaatgatga agaaatacat ctattggcta gagatatgac taagaaaata
    32821 gaggatgaac ttgaatatcc tggacaaata aaagtaagca taataagaga aacaagagct
    32881 atagagtatg cgaagtaaaa gaaataatat ctaaataata agaaaatatt tttagaaatt
    32941 taaatttgcc acaagatatg ataatatatc ttgtggcttt taaattgttt aagtttaatt
    33001 ttatataatg caaaaataat ctaagtgcta aaagtgctac ttacaaaaga ttaataaaat
    33061 aaagctttat atatgtatta tt
//
LOCUS       DAMJWP010000016        32194 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.017, whole genome shotgun sequence.
ACCESSION   DAMJWP010000016 DAMJWP010000000
VERSION     DAMJWP010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32194
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.017"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(140..1498)
                     /locus_tag="PC700_000706"
     CDS             complement(140..1498)
                     /locus_tag="PC700_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2580030.1"
                     /translation="MFIRKNVIKLAIPIMIEQTFVMLLGVFNTIMAGHIGEEAVSAVG
                     MVDSINNIFISVFAALSVGATVVVAQQIGKKDSEKVNETIKQALVSGVILSFTITILM
                     WVFRTGVINLFYGSAEELVKSNAKLYIEFTLFTYPFIATQQIANGILRGCGDVKKPMY
                     VTMFMNIVNVTLGYILIYGIDLNPLGINLQTPSYGITGAAISIAIARIIGCIVIIRAL
                     FKGNEFISMGKIFPFKIDVETQKSIFNIGIPAGVEQLLFNAGKLIVQVFIVTMGTSAI
                     ASNAIGMSIASIINVIGNALALSATTLVGQYIGRGDIKGAKSTLLYLTKFATICLFIV
                     GAIFIPIAPWISSMYTQNASVIDISTQLIRSDCLAMVIWPISFVLSAGLKGAGDTRYT
                     MMTAVIGMWIFRIFTGYVLGVVLEFGVLGIWIGMYTDWLVRGTLYCLRLRGTKWLKHK
                     VA"
     gene            1946..2398
                     /locus_tag="PC700_000707"
     CDS             1946..2398
                     /locus_tag="PC700_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staygreen family protein"
                     /protein_id="HDF2580031.1"
                     /translation="MVNLNANNLNVNMVYPISSTAPIRFRRYTVSYLENMENIYLTIA
                     TYFDTLGLGTMNIDKVYGQWAWFTDNIYDLNLFVFVGNYPYQIAKNRYDTFVDILPVA
                     ITSIVNGDRVFLISNPCLLNSKITVRFISSHPTLNRTVDYGNIRDFIF"
     gene            complement(2555..3172)
                     /locus_tag="PC700_000708"
     CDS             complement(2555..3172)
                     /locus_tag="PC700_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HDF2580032.1"
                     /translation="MEIFAIGHSNYPYDKLINMIKKYDINCVVDIRETPYSKYNIQYN
                     KEAFNESLRNSGFIYIYMGKEFGAKRNNKDVYTQEGYADFEKVAKEDIFLNGIERLKK
                     GCQMGYRIVLLGAMQEPIRCHRSILVGKVLNKEGFDVKYIMHEGNLAYQEDIEESLLD
                     KYFSDREQLSIDNLLGSALTREEMIQEGYKLANKEIGYRTEKLGK"
     gene            3721..6381
                     /locus_tag="PC700_000709"
     CDS             3721..6381
                     /locus_tag="PC700_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HDF2580033.1"
                     /translation="MKKILKRLCTGFLAFATVVTALPSSTVHASNTQYWTESKERVSI
                     VEQVMNDGSISSTFNEGHLTVEGEDAYCIDINTAFKNGYKTKADASTRMSADQIADVA
                     LSIEYVKQYTDIHSGISSKHAYLLRQLVVWQRLSVQPDWQWDNVRASYDEIPKAVQDE
                     VFAGAKAFVKENKGRYDCGGYIYSGEGQELGQFWAKLAVGNTKLQKTSTNANITDGNG
                     IYSIAGATYGVYSDKDCTKQLTTLTTDTSGNTEAVEVRATIVYIKELSAPAGFKIDKT
                     VYSLSVEVGKTATLKVSDTPKVTDTLIELFKIDMETQKSNPQGNASLEGAEFTWNFYA
                     GYYNKNNLPAQPTRTWVTKTIAEKDSDGAIHYITRLADKYKVSWDSFYTQDGKNVLPL
                     GTLTVEETKSPSGYLLEGTYMQADGSEEQIKGMYLTQITEDGDLAVLSGSNQYHVSDK
                     VIRGGVKIQKRDLETKDTKAQGGATLKDTAFEIISLNENAVLVEGKLYKKNEVLKTIH
                     TDIEGIASTSADLLPYGKYRLSEQKPPEGYLTDGAKPIDFEITENGKIVDLTDEAHSI
                     YNQIKRGDIEGVKIGAGSHKRLADVPFRITSKTTGESHVVVTDDNGQFSTASDWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALLYDTYIIEELRCESNKGFKLIPPFEIVISRN
                     KVVVDLGTLTDEYEKEITIHTTATSKDGEKTILAGKDVTITDTVKLDGLTKGTKYQLK
                     GWQMLKEENAELIINDKRIENDYIFTADSETMEVKIEFTFDASNLGGKQLVTFEELYD
                     LSNPDEPIKVAEHKDIEDDGQTVTIKEVPETPTPEEPEKPTTPDTPTKTDSPKTGDNT
                     NILAFAIMMFVSAGGLAGTYFFKHRKMKKS"
     gene            6606..6929
                     /locus_tag="PC700_000710"
     CDS             6606..6929
                     /locus_tag="PC700_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDF2580034.1"
                     /translation="MELKYIVPNVAQTFGNLEFAGENKVDQQRINGRYTLVSRSYNLY
                     SDIQRADDIIVVLPAKAGEKHFKPEQKVKLINPRITAEGYNVKGRGFTNYILMADDML
                     PAESK"
     gene            6944..7327
                     /locus_tag="PC700_000711"
     CDS             6944..7327
                     /locus_tag="PC700_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDF2580035.1"
                     /translation="MRLTNGIVLDNEKTFGVLKFSALRHEVRVENEDGTTSDEIKRRT
                     YDLKCSVQERMIQVSIPADVPVKDFPYNAEVELINPVADTVANANFRGTTVDWYIKAD
                     DIVLKNKGGQAGNPQNNAPQGQQKK"
     gene            7423..7836
                     /locus_tag="PC700_000712"
     CDS             7423..7836
                     /locus_tag="PC700_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HDF2580036.1"
                     /translation="MLDKIPNAEEMTALVGQSLYSVWNELCALIDEKYEMECLWNKGG
                     KAWNYEYKYRRGGKTLCALYARENCVGFMIILGKDERLKFDTYRDNYSKEVQRIYDET
                     KTYHDGKWLMFEPTDTTLFDDFVKLLRIKRKPNRK"
     gene            7943..8401
                     /locus_tag="PC700_000713"
     CDS             7943..8401
                     /locus_tag="PC700_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2580037.1"
                     /translation="MKLKNPMLVVTDIDKSVEFYKKVFGLYVIMDFGANKTLTGGLAL
                     QTSETYKEFIGTSDISFGGNNFEVYFEEEDFDKFADRLKEYDIEYVHPIIEHSWGQRV
                     VRFYDPDKHIIEVGENMKIVCKRFLNSGMTPEQVAERMDVPMKFINACMR"
     gene            8426..8968
                     /locus_tag="PC700_000714"
     CDS             8426..8968
                     /locus_tag="PC700_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HDF2580038.1"
                     /translation="MKGFERKNQLFSLCGLNCGLCPMLLGNYCGGCGNGNQSCRIAKC
                     SLEHGQIEYCYECKQYPCEKYQHIDDYDSFITHKRRKADLERAKNIGIEQYNLEQQEK
                     TQILSYLLSNYNDGRRKTFFCVAVNLLELSELQEAMKQIQSNDELTLLPFKAQCLYVV
                     EVFQKIAERRNIKLKLFKKK"
     gene            9004..9531
                     /locus_tag="PC700_000715"
     CDS             9004..9531
                     /locus_tag="PC700_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HDF2580039.1"
                     /translation="MTNGGIPLETVELRFQYTQSEYIKAERQYLISSKTIHKYDIALV
                     AVFLLLSVVYMLFSSFSVFSILIFGLIIVVTALGSYLYILMPILKFKQTAKYHEEYTL
                     VFSKENIKFKTQSIDSEVKWDIYSALWESHDFYYLIQAPRLYTLIPKRIFKDLNEKQL
                     FEEIAQSRVKTTKHI"
     gene            9641..11035
                     /locus_tag="PC700_000716"
     CDS             9641..11035
                     /locus_tag="PC700_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2580040.1"
                     /translation="MRMILNKGHRIRARDKNLVYHFSIGTLLFVFVAVLLLLNIKQLM
                     RTDWEHFSLLDNGLTLSPYNFITILIATGICALVAFLYYRFCYDSFKKLLHRQKLARM
                     ILENKWYEADTVQDSGFFTDLQSRSREKIVWFPKIYYQMENGLLHIHCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTLLYDMIANRISIDEVKAENGCLRLMKNLV
                     WEYDVLPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMGNVY
                     HTKEDMIDCVNAFYEGMVQRSEEMKQHPDYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVSLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQTIGSLAFVRQ
                     DGTATCEAKGDGTD"
     gene            11315..12436
                     /locus_tag="PC700_000717"
     CDS             11315..12436
                     /locus_tag="PC700_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDF2580041.1"
                     /translation="MAGISREHLSRIEAGKVALTEDMKHKLLEAVEKFNPDAPMFLLF
                     DYVRIRFPTLDIQHVIKDILKLNIDYMLHEDFGHYKYTEHYYIGDVFVYTSQDKEKGV
                     LLELKGKGCRQFESYLLAQERSWYDFLMDALVDGGVMKRIDLAINDRTGLLDIPELIE
                     KCKNEECISKFRSFKNYGSGELVKHNEANKDGMGHTLYIGSFTSEVYFCVYEKNYEQS
                     SKLGIPIEEVPIKNRFEIRLKNEHAYYAIRELLTHYDAELTAFSIINQYIRFADKEAN
                     KRKSDWKTNARWSWFIGEGRPPVKLTTKPEPYTLQRTLNWLQRQVAPTLKMLKQIDAG
                     NQMDYLKTIEQQAKLTDKHKQIIRQQTASVDELIEKEKE"
     gene            12443..12577
                     /locus_tag="PC700_000718"
     CDS             12443..12577
                     /locus_tag="PC700_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HDF2580042.1"
                     /translation="MWELIKDLLLVSLGMGIGVVLMCIMSVGKEADRHMIELKESEEK
                     "
     gene            12578..12799
                     /locus_tag="PC700_000719"
     CDS             12578..12799
                     /locus_tag="PC700_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580043.1"
                     /translation="MNFGQNLYTWFLSNAQSLVLMAIVVIGIYLGFKREFSKLIGFLV
                     VALVAVGLVFNAGGVKDVLLELFNKIIGA"
     gene            13119..13757
                     /locus_tag="PC700_000720"
     CDS             13119..13757
                     /locus_tag="PC700_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase CAT"
                     /protein_id="HDF2580044.1"
                     /translation="MEYKYTKIDLNKWNRGKLFQSYIDNMRIVMSLTVDIDVTKFIEF
                     SKKHDLKFYPAMIWAVSKVVNEHDEFKYSWDNNGNLIKWDYISPSYTEFHKEDENFTK
                     IVTDFSDSIFEFHKRFILDREKYKTERAFVENQSLNFFDVSCLPWVKYNHFDVHVFDE
                     GKFLAPVITWGKYELEQERYIMPLTMNIHHAVADGFHLSRFFNEVQELINSI"
     gene            13834..14022
                     /locus_tag="PC700_000721"
     CDS             13834..14022
                     /locus_tag="PC700_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580045.1"
                     /translation="MKDIFSDMQASIGCTYISDLPHHKRMVWQEMKRLNLADYPQKQL
                     EDFAGYVFGVPYTSIAEA"
     gene            14043..14540
                     /locus_tag="PC700_000722"
     CDS             14043..14540
                     /locus_tag="PC700_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDF2580046.1"
                     /translation="MNEMRVCIESRLHTSEEIRSAWFTLPIDEEELEEQIGVPIDSED
                     YHIVEKELPFADEVGEDATIEKLNDLYHTFESLPADLQEDYGELMCYFTSLDELHQHR
                     HDVIHYSWCKNMADVARHLLDNDPAFSAIEERLTRYFDYEAYGQYLDDNGEFIETDHG
                     IYELP"
     gene            14556..15059
                     /locus_tag="PC700_000723"
     CDS             14556..15059
                     /locus_tag="PC700_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDF2580047.1"
                     /translation="MVEDMRVYIANLGKYNEGYLVGDWFSFPIDEEDVAERIGLNERY
                     EEYAVHDTENFPIEIGEYISIEELNEMYEMICELPDYITDSLDEFVSNYGSLEELYDH
                     RDDMILYAGCEDMTDVAYYFIDELQALGDIPPSLQNYIDYEAYGRDLSFEGTFIETRY
                     GMCEIPW"
     gene            15172..15561
                     /locus_tag="PC700_000724"
     CDS             15172..15561
                     /locus_tag="PC700_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2580048.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDLPPLSMIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITFALRPKITY
                     AGKAVKLDKQVLHEAITAVRSVSYVPD"
     gene            15548..17980
                     /locus_tag="PC700_000725"
     CDS             15548..17980
                     /locus_tag="PC700_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2580049.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSSRSIQEQSKKLVTGRLKDVAYQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTDQSMNLKSVKQSAFLTFKEFIHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLDKNDFGYLIEHIYGRDGVAYEDYEYSLPKKKLKKE
                     TLITHYDLIRPTRCLVEESQRHLRLEHEDSESYVSYFTVNAIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALSTVRNKKKELKDLDNHAYQSGSETSSNVVDALDSVD
                     ELETDLDQSRESMYKLSYVIRVSAPDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGENTGIYIGYSVDTGRNVYLQPS
                     LASQGVKDTVTNALASAFVESLGGGKSFCNNLLVYYSVLFGGQAVILDPKSERGNWKE
                     TLPEIAHEINIVNLTSDRENAGLLDPFVIMQNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRAVTQSDQRGLLHVIEELRKEDTPISCNIADHIDSFTDYDFAHLLFSDGTV
                     QNAISLDNQLNIIQVADLVLPDKDTTFELLSVSMLIVISTFALDFIHSDRSVFKIVDL
                     DEAWAFLNVAQGETLSNKLVRAGRAMNAGVYFVTQSSGDVSKESLKNNIGLKFAFRST
                     DINEIKQTLEFFSIDKEDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFEELLHAF
                     DTRPPIQRNEVE"
     gene            17980..20115
                     /locus_tag="PC700_000726"
     CDS             17980..20115
                     /locus_tag="PC700_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDF2580050.1"
                     /translation="MKERIKSVFTRKKIFRFLKMALFVVALSLILLSLLGTVAHATGL
                     VDDTINTENLYSKYPLSNYQLDFYADNNWAWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     RLYLSNATGYVVQEAYKLDFINDMADSIGRSIQTLAGVTQNGFSTSGFYVGFLLLIIL
                     VVGIYVAYTGLIKRETSKALHAVINFVVVFVLSASFIAYAPDYIKKVNDFSSDISTAS
                     LDLGTKIMLPDSDSKGKDSVDLIRDSLFSIQVQQPWLLLQFGNSGIEEIGADRVEALV
                     SASPEDEDGKTREEVVKTEIEDNDNNNLTILQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVYFAGIYMKLGDLMSMFSLNAGDSQSMGRRIFRRP
                     YLFMRHRARRMEHRLARAVSAGGVAGAVAGGVVASSKSTRQNGTKNNRGNTSSSMGQK
                     AGSAVGAVLDTKNKVKDKATAVKENIKDMPTQTAYAVHSAKEKAKSSVSDFKRGVVQE
                     KENRQSGRLKKQQLHRQNIADKRMELQKAQESRQAGKKTDGSATSGATRPHERPATTS
                     KVNVEKQQEVKRPAIASTVKNDEPIKKNVVKERPLSSGDTSAKTVNQETTRQNYKTDR
                     VTKTTVQKHTQQNTEKNKRTVFKKGQKKK"
     gene            20112..21119
                     /locus_tag="PC700_000727"
     CDS             20112..21119
                     /locus_tag="PC700_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDF2580051.1"
                     /translation="MKLRHIAIVGSLFSILFSLVLFFGVLISAEDDDGGNGYSSNYVG
                     MNLSAEVLKHQPMVEKYAKENGISEYVNVLLVIIQVESGGTAEDVMQSSESLGLPPNS
                     LDTESSIKQGCKYFASLLSSCKNQGISDINVVIQSYNYGGGYVGYVAGNGKKHMFNLA
                     ENFARDKSGGQKVTYTNPIAVAKNGGWRYGYGNMFYVELVNQYLVVNQVSGELAQKVM
                     NEALKYQGWKYVYGGSNPNTSFDCSGLVQWCYNKAGVSLPRTAQNQYDATQHIPLSQA
                     KAGDLVFFHSTYNAGSYVTHVGIYVGNTQMYHAGDPIGYADLNNAYWQQYLICAGRIK
                     Q"
     gene            21135..22028
                     /locus_tag="PC700_000728"
     CDS             21135..22028
                     /locus_tag="PC700_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2580052.1"
                     /translation="MFKKKEKPVKETKVRTMKVGTHKKSVIALWVVLIASVSFGVYKN
                     FTAIDMHTVHETETIQLRLNDTNGVENFVKNFCKSYYTWDNSKEAIEARTQAISNYLT
                     KELQDLNLDTIRTDIPTSSTVTNVLIWDIKQSGTNDFIATYEVDQQIKEGEQTSNVKA
                     TYTVKVHVDKDGDMVIVQNPTIAPAIEKSDYEPKAQEADASVDADTVNDATAFLETFF
                     KLYPTATEKELAYYVAGNVLEPIGRDYLYSELVNPMFTDDRDNVKVRVAVKFIDNQTK
                     AMQVSQFELVLHKDSNWKIIM"
     gene            22241..23098
                     /locus_tag="PC700_000729"
     CDS             22241..23098
                     /locus_tag="PC700_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2580053.1"
                     /translation="MYKEVGISSNNVPITLSIWYTERNSPTIVFLPGTMSHPLMYENF
                     LCGLSERGFNIIGIHYLSHGKSPKIKKNYTMEDLLHNVYDATTYGIENFGENIAVMGS
                     SQGGILAAMAAGRDTRLKAVFPHNIMLTTLKETMSLTKYPAWSHRFMGLIQLGFRVGG
                     KLLPNYKVPGDAYLDYDRVFYSEQAKQDCLNDPMLLPYYPLRFVASLFNADLSCLENG
                     AIQCPVILITAKGDPLFRFDYTNKVFDLIHVPKKELLTLELNRHMIFNEDTDTTINAL
                     EATLHKYLG"
     gene            23176..23757
                     /locus_tag="PC700_000730"
     CDS             23176..23757
                     /locus_tag="PC700_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2580054.1"
                     /translation="MPRFTEQEKEIINSKLLIEGEKLFSLYGLKKVTVDDLAAAVNIS
                     KGSFYAFYPSKEHLYVEINFRLQKELFTSIETTVKKKKYKNHRDLAKDVIMLSLTGVI
                     TSPILSQIDLSLMDYLQRKISSDIFENHMYSDIRILEILEGLGVVFLVPHTVIIKSLY
                     SVLSCLEQFKEDEELNMVQNLLVNGIIQQVVAE"
     gene            23861..24706
                     /locus_tag="PC700_000731"
     CDS             23861..24706
                     /locus_tag="PC700_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2580055.1"
                     /translation="MIDVKKLYFSYTDKPFVENVSFHVGRGEIFGFLGPSGAGKSTIQ
                     KILTGLNTSYKGSVKVAGTEIREHTNRFYENIGVDFEFSTCYEKFTARQNLAYFASLY
                     DKQPRSIDELLHMVGLENDGDKKVADFSKGMRSRLNFIKALVHDPDILFLDEPTSGLD
                     PTNNRLMKDIILAEKKRGKTIIITTHNMYDATELCDQVAFIVAGKVSALDSPHNLIMS
                     RGAAKIHYTYYDKGEKTGECLLDRTTEDKLLKSLISENRLLSIHSSEPTLNDIFVDIT
                     GRTLQ"
     gene            24703..25386
                     /locus_tag="PC700_000732"
     CDS             24703..25386
                     /locus_tag="PC700_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2580056.1"
                     /translation="MRLGRLICGDIHFQWKYGFYFIYFILTVLYVCGIAALTGHWKTD
                     IASIMIYSDPAAMGLFFMGAIVLLEKSQKVLNAMVVSPVKVSEYILSKTIALIAISTI
                     IAVILGFVSGSNHLLGIAVGTALTSAIFTMLGIIAATKISNLNQFLIVIMPIEIVCFV
                     PPIVGLFVKLPDIFRFFPFTACMNLITGKSILLSFDMVLVIATLIILYIVARHTVKHM
                     WRSLGGVKL"
     gene            25383..26081
                     /locus_tag="PC700_000733"
     CDS             25383..26081
                     /locus_tag="PC700_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2580057.1"
                     /translation="MKRILSSTIQVFKQIKSDPMMFAACFTPFVMGALIKFGIPFLDG
                     ITDFSLQTYYPIFDLLLSIMAPVLLCFAFAMITLEEIDDKVSRYFSITPLGKSGYLFT
                     RLGVPSIISAVIAFIVLLLFSLEKLSIGMTICLALLGSVQAIIVSLMIITLSSNKLEG
                     MAVTKLAALTLLGIPAPFFIDSYYQFAVGFLPSFWVAKAMQNEAVLYFSIGLMVALVW
                     YYFLIKRLFRKLAG"
     gene            26093..27004
                     /locus_tag="PC700_000734"
     CDS             26093..27004
                     /locus_tag="PC700_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDF2580058.1"
                     /translation="MEKGRLLQNENINKAIQVSKITLIINLGLSLLKFAAGYIGKSSA
                     MLSDAVHSASDVLSTIVVMVGIKISEKQPDKEHPYGHERMECVASIILSVALAITGAG
                     IGYSGIKKIFSGQYNTLSVSSGIALTAAVLSIVIKEWMYWYTRSAAKHTNSDALMADA
                     WHHRSDALSSVGSLIGILGARLGYAILDPIASIVICGCILKAALDIFKESINKMVDHS
                     CDNATETKIREVVLQQQGVDGIDELKTRMFGAKMYVDIEILADGNLALYDAHRIAEGV
                     HQTIENNFPQCKHCMVHVNTNELPYSP"
     gene            27057..27197
                     /locus_tag="PC700_000735"
     CDS             27057..27197
                     /locus_tag="PC700_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580059.1"
                     /translation="MEQYWWIIIVMVLIVVGLLKIVIMKKYNQRRNSNNRSKNSEDDK
                     MY"
     gene            27312..27503
                     /locus_tag="PC700_000736"
     CDS             27312..27503
                     /locus_tag="PC700_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HDF2580060.1"
                     /translation="MKKYEYVEINYNAKGVVFLCTDKHKEIIDSYAKTGYHYAGFVPT
                     EIDAKGCMRKIDLIFEKED"
     gene            27527..27757
                     /locus_tag="PC700_000737"
     CDS             27527..27757
                     /locus_tag="PC700_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580061.1"
                     /translation="MIGLKRRDMNYIMKVGAKAQGVSVSEFRAMLQDTIDKTMESTDP
                     DVQANFKRYFGNKRPTPEEYIYTITKKTKVKV"
     gene            28023..28313
                     /locus_tag="PC700_000738"
     CDS             28023..28313
                     /locus_tag="PC700_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580062.1"
                     /translation="MKPSDFQKTIQCQFECKLKKVVKGIIWNYRKELRRRKNKEISFS
                     ELPDITVESLATWDDYETNYTIFNVCGIDIKVLDDELAEALKQLPEKNVTMY"
     gene            28313..28447
                     /locus_tag="PC700_000739"
     CDS             28313..28447
                     /locus_tag="PC700_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2580063.1"
                     /translation="MYYFLEMSDTEIADIMNYTREAVFKHRHRALETMKEILNEKTRQ
                     "
     gene            28428..29609
                     /locus_tag="PC700_000740"
     CDS             28428..29609
                     /locus_tag="PC700_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDF2580064.1"
                     /translation="MKRQDSKGRNLRLGESQRKDGRYVYKYTDIFGKPQFVYSWKLVP
                     TDKTPAGKRDDISLREKEKQIKKDLDDGIDTVGGKMTVCQLYEKKNNQRKNIKRNTEL
                     GRQYLVNALENDPLGMRAIDTVKQSDAKEWAIRMNGKGYSYKTIDNYKRSLKASFYMA
                     IQDDCIRKNPFNSKLSDVLEDDTEAKVILTPEQEEKLLAFMETDNVYSKYRDEVILLL
                     ETGLRISELCGLTTHIDMLNRVINIDHQLLRDTEVGYYISKAKTKNGKRELPLTERAY
                     QALKRILKNRGKAQPLVVGGYSNFLFLNREGLPKVAGNYESMVRGLIKKYNKTHKDKL
                     PNITPHSFRHTYCTNMANKGMNPNTLQYIMGHANITMTLGYYAHGTFQSAKAELERLA
                     S"
     gene            complement(29949..30761)
                     /locus_tag="PC700_000741"
     CDS             complement(29949..30761)
                     /locus_tag="PC700_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2580065.1"
                     /translation="MFSIGMFSKINKITTKTLRYYEDIGLLKPEYVDEFTKYRYYTTE
                     QLPKLHQIITLKQMGLSLAEIKKAVENPEEVENILMNKEKEMLDVIKSEEYKIIKLRN
                     YLMNLKGEVNMNDVIIKSLPKVKVASMRKVLKGYDELFNLFPNVMAEEMMKTGCTCAV
                     PDYCFNIYHDGEYRETDMDVEMCQAINDLKEDTDILKFKELDAVENAVCILHNGPYST
                     LGKTYADAFKWIEDNKYKVVGNPRESYIDGIWNKESEEDWLTEVQIPVKRIL"
     gene            complement(30837..32072)
                     /locus_tag="PC700_000742"
     CDS             complement(30837..32072)
                     /locus_tag="PC700_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HDF2580066.1"
                     /translation="MGLIFIMFFGGFGTNWNMAKPVAIPSILMSSLGVIITSGVTGMF
                     CHMVIGTSLLEGLLIGAIAASTDAASVFAVLRSQKLNLRGSLASLLEVESGSNDPIAY
                     MLTLILLTLMNNSGVELIAPMVIKQIAFGLAIGFILAKLSVYILRHSNFEIEGFYTIF
                     ITAIAILSYAFSEYLGGNGYLSVYISGIIIGNSRIPLKKSLVHFFDGISWIMQIMLFF
                     MLGLLSFPSKLPNVFGIAMAISIFMIVIARPLATFIVLAKFKFSNKEKLFISWVGLRG
                     AASVVFAIYAVTQGVAIENDIFHIIFFIALLSVGIQGSLIPTVARKLDLVDDNTPVLK
                     TFNDYDGEISSKLIEVNVDEGNKWANKSIIDSNIPDDILIVMIKRKEQVLIPKGSTII
                     KTGDILVLSGDRIEELIQL"
BASE COUNT        10538 a         5737 c         6377 g         9542 t
ORIGIN      
        1 aatttataat atccatacta ttatagcctt tatttatgta atttaataac cttatatatc
       61 atacttataa ttatgataat aactacataa aaaatcactt ctaaatagag ttatccatct
      121 aaaagtgatt tcatatattc tatgcaactt tgtgttttag ccactttgtt cctcttaatc
      181 ttaaacaata taaagtccct ctaacaagcc aatcagtata catacctatc caaattccta
      241 acactccaaa ttctaataca acccccaata cgtaacctgt aaaaattcta aaaatccaca
      301 tacctattac tgcagtcatc attgtatatc tagtatcacc ggctcccttt agccctgctg
      361 ataatacaaa ggatataggc catattacca tagctaaaca atcacttcga attaattgtg
      421 ttgagatgtc aattacacta gcattttgag tatacatact tgaaatccag ggtgcaattg
      481 gtataaatat agctcctact ataaataaac aaattgttgc aaatttagtt aaatataata
      541 aagtactctt agctccttta atatctccac gtcctatata ctgtcccacc aaagttgtag
      601 cagacaaagc taaagcattc ccaattacat ttattatgga tgcaattgac attcctattg
      661 cattggatgc aattgctgat gttcccatag taactataaa tacttgaaca atcaatttac
      721 ctgcattaaa aagtagttgt tcaactccag ctggtatccc tatattaaat atagactttt
      781 gagtttcaac atcaatctta aatggaaata ttttacccat agatataaat tcatttcctt
      841 taaataaagc tcttataatt actatacatc caattattct agctatagca atagatatag
      901 ctgcacctgt tatgccatag gatggtgttt gcaaatttat tcctagagga tttaaatcaa
      961 taccatatat aagtatatat cctaatgtta catttactat attcataaac atagtcacat
     1021 acataggttt ctttacatct ccacaccctc ttaaaatacc atttgctatt tgttgtgtcg
     1081 caataaatgg atacgtaaat aatgtaaact ctatgtataa ttttgcattt gattttacca
     1141 attcttctgc tgaaccataa aataaattaa ttacaccagt tctaaaaacc cacatcaata
     1201 ttgttattgt aaaggataat attactccag acactagagc ctgcttaata gtttcattca
     1261 ccttttcact atcttttttt cctatttgtt gagctacaac gacagtagct ccaacagata
     1321 atgcagcaaa tacagatata aatatattgt ttattgaatc aaccattcca actgcagata
     1381 cagcttcttc cccaatatga cctgccatta ttgtattaaa tactcctaaa agcatgacaa
     1441 acgtctgctc tatcattata ggaattgcta actttataac atttttcctt ataaacatcc
     1501 ctattcctct tttccctctt tatataaatt taatttttaa ttagtttatc gaactgtaaa
     1561 ttgacaatgc aaagtttaga attgtattgt tcgcattata atatactgct ttcaaataat
     1621 ataaatcttg tttaatatca tttgaacaca gtatatcttt ataattatct tatgtttatt
     1681 attttttatg gataccacat ttaaaatatt tattaattta atataaatat tttccgttat
     1741 tattaaaagt agctatattt cgttaatatt gtatcactat cgtgtagcaa taacaataag
     1801 tacattttat atatttttta taatattatt taatcatcca aacttttacc actttttcaa
     1861 tatataaaat attacacttt agatgtaaca aaatcgcatc catttaaata ttgtatttta
     1921 tagactaatt ttaggatgtg atactttggt taatttaaat gcaaataatc taaatgtaaa
     1981 tatggtttat cctatttctt ccacagcacc catacgtttc agaagatata ctgtttcata
     2041 tttagaaaac atggaaaata tttatttaac tatagcaaca tactttgata cacttggttt
     2101 aggcacaatg aatatcgaca aagtttatgg tcaatgggct tggtttactg ataatatata
     2161 tgatttaaat ctatttgttt ttgtaggaaa ttatccttat caaattgcta aaaacagata
     2221 tgatacattt gtagatattt tgccagtagc tattacttct attgtcaatg gagatagagt
     2281 atttttaatt tcaaatcctt gtttgttaaa ttcaaaaatt acagttagat ttatatctag
     2341 tcatcctact cttaatagaa cagttgatta tggcaatatt agagatttta ttttttaaaa
     2401 ctgcaacatg atgtgctatt aaaaaatacc ctaaatttaa tgatattatt aaataactag
     2461 aatattttta attggcacat catgcctagc tattataaat tcataatatt attttactta
     2521 agataagtac tgtattttat ctacttgtat atttttattt acctagtttc tcagttctat
     2581 atcctatttc tttattagct agcttataac cttcttgtat catttcttct ctcgttaatg
     2641 cactccctaa taaattgtca atactcagtt gctctctatc actaaaatac ttatccaata
     2701 atgattcctc tatatcttcc tgatatgcca aatttccttc atgcattata tattttacat
     2761 cgaaaccttc tttatttaat acttttccca ccaaaatact tctatgacat cttataggtt
     2821 cttgcatagc tcctaataaa actatcctat aacccatttg acaacccttt tttaatcttt
     2881 ctattccgtt taaaaaaata tcttcttttg ctactttctc aaaatctgca taaccttctt
     2941 gcgtatatac atctttatta tttcttttag ctccaaattc ttttcccatg tatatatata
     3001 taaatccgga atttctaaga gattcgttaa atgcctcttt attgtattga atattatatt
     3061 tagaatatgg agtttctcta atgtcaacta cacaatttat gtcatatttt tttatcatat
     3121 taataagctt atcatatgga taatttgaat gtcctattgc gaatatttcc atataaatac
     3181 cctcctattc tttgaaatta gtatctatat atgtagatac ccgatttact taaaattaaa
     3241 taaagtatta ctttaaatac ccaatatctt tttttcttga tactgtatat ttttttactt
     3301 tatatcctta aaaaaatata caactttata tataaaacat caatatttat atatattttg
     3361 tatattaaat acaattattt ataaaatctt tgataaaaat ttataaatag agcatcttta
     3421 gaaaactatt aacagaaaaa cacactaaaa atttgattat ccgctaaaac actgtatcta
     3481 tcggcattat caaggagcta aacacatcaa aatactacta atttactacg attgaatgag
     3541 ccttgatacg tcctatttcc cagatatgca aagatacggt acagcacatc agtattgaaa
     3601 caagaaaaat tgaatacttc atagactacg gaacgcacaa tatcagcgtt ccttttctat
     3661 ttccagccgt tcctagtgga cggctatttt actaccaaaa tgaaaggagc atttagaatt
     3721 atgaaaaaga tattaaaacg attgtgtaca ggctttcttg cctttgcaac tgtcgttacc
     3781 gctttaccga gttcaacggt tcatgcgtct aatacacagt attggacgga aagtaaagag
     3841 cgtgtcagta tcgtagaaca agtaatgaat gacggttcta tcagttctac ctttaatgag
     3901 ggacatttaa ccgttgaggg tgaggacgct tattgtatcg acatcaacac agcctttaaa
     3961 aacggctata agaccaaagc tgacgcaagt acccgtatga gtgccgacca gatagctgac
     4021 gtcgccttat ctattgagta tgtgaaacag tacacagaca tccacagcgg aataagcagt
     4081 aaacacgcct acctattaag acagcttgtt gtctggcaga gattaagtgt tcagcctgac
     4141 tggcaatggg ataatgtgcg agcttcctat gatgaaatcc caaaagccgt acaagatgaa
     4201 gtattcgcag gagcaaaagc ctttgtgaaa gaaaacaaag gacgttatga ctgtggcgga
     4261 tatatctact ctggcgaggg tcaagagtta gggcaattct gggcgaagtt agctgtcgga
     4321 aataccaaac ttcaaaagac ttccaccaat gccaatatta cagacggcaa cgggatttat
     4381 tcaatcgctg gtgcaacata cggtgtgtat tctgataaag actgcacgaa acagcttacc
     4441 acccttacga ctgatactag cggaaataca gaagccgttg aagtaagagc cactatagta
     4501 tatatcaagg aactttctgc accagcagga tttaaaattg ataaaacagt ttactcttta
     4561 tctgtggaag ttggaaaaac agcgacttta aaagtatctg atacaccaaa agtaacagat
     4621 accttgattg agctattcaa gattgacatg gaaacacaga aatccaatcc gcagggaaac
     4681 gcttctttag agggagcaga gtttacatgg aacttctacg ctggatatta caacaaaaac
     4741 aatcttccag cacagcctac aagaacatgg gtaacaaaga ctatcgctga aaaagacagc
     4801 gacggtgcta tccattacat tactcgttta gctgacaaat ataaagtatc ttgggacagc
     4861 ttctatacgc aggacggtaa aaacgtactt ccattaggaa cactaactgt tgaggaaaca
     4921 aaaagtccgt cgggttactt gttagagggt acatatatgc aggctgacgg tagcgaagaa
     4981 cagataaaag gaatgtatct gacacagatt acagaggacg gcgaccttgc cgtattatct
     5041 ggaagcaatc aataccatgt ttctgataaa gtcattcgtg gcggtgtgaa aatccagaaa
     5101 cgagatttag aaaccaaaga cacgaaagct caaggcggtg cgactttgaa agataccgcc
     5161 tttgaaatca tttccttaaa tgagaatgcc gtcttagttg agggcaagtt atacaagaaa
     5221 aatgaagtgt taaagacaat ccataccgac attgagggta tcgcttctac ttctgctgac
     5281 ctattacctt acggaaaata cagattgtct gaacaaaaac cgccagaggg atatttgact
     5341 gacggtgcaa agccgattga ttttgaaatc acagaaaatg gaaaaatcgt tgatttgact
     5401 gacgaagccc actctatcta taaccagatt aaacgtggcg atattgaggg cgtaaaaatt
     5461 ggtgcaggtt cgcacaagcg tcttgcagat gttccgttta ggatcacaag caagacgacg
     5521 ggcgaaagtc atgttgttgt aactgatgat aacggacaat tctctactgc ttctgattgg
     5581 gcttcccaca agcacaatac caatgcagga aaaacaagcg aggacggtgt gtggtttggt
     5641 acttctgaac cagacgacag caaaggtgcg ttgctttatg atacttacat cattgaagaa
     5701 ttacgttgtg aaagcaacaa aggtttcaaa cttattccac cgtttgagat tgtcatttcc
     5761 agaaataagg ttgtggttga tttaggtacg ctgactgatg aatacgaaaa agaaatcact
     5821 atccatacta ctgctaccag caaggacggc gaaaagacta ttcttgctgg gaaagacgta
     5881 actatcactg atacagttaa attagacggt cttaccaaag ggacaaaata tcagttgaaa
     5941 ggctggcaga tgttaaaaga agaaaatgca gagcttatca tcaatgacaa gcgtattgag
     6001 aatgactaca tctttacggc tgacagcgaa actatggaag ttaaaatcga gtttaccttt
     6061 gacgcttcca atcttggcgg taaacagctt gtaacttttg aggaattata cgatttaagc
     6121 aatccagacg aacctatcaa agttgcggaa cacaaggaca ttgaggacga cggacagaca
     6181 gttactatca aggaagtacc agaaacacct accccagaag aaccagagaa gccaaccaca
     6241 ccagatacac ctacgaaaac agacagtccc aaaacgggcg acaatacaaa tatccttgcc
     6301 tttgcaatta tgatgtttgt ttctgctggt gggctggctg gtacatactt tttcaaacac
     6361 cgtaagatga agaaatcata aggcggtagc tgtatgagaa aagaaaaatc acgctctccg
     6421 ccatagctta gataggcaga ctatacctat tctggcttgt ctgccgatta cctgcaagaa
     6481 cacggatagt caagggtgcg ttagcattta tttacccttt actatctggg ggcttgctgg
     6541 cactttccta aatgccagaa tacacacatc aatcaaactt aaagtaagaa acgaggtaaa
     6601 ataatatgga attaaaatat atcgtgccga atgtggcaca gacttttgga aacttggaat
     6661 ttgcagggga aaacaaagta gaccagcaac gtatcaatgg gcgttacact ctcgtatcaa
     6721 gaagttacaa cttgtattcg gatattcaga gagccgacga cattatcgtt gtattgcctg
     6781 caaaagctgg cgagaagcat tttaagccag agcagaaagt aaaactgatt aacccacgca
     6841 ttaccgcaga gggttacaac gtcaaaggac gtggatttac aaactacatt ttaatggctg
     6901 atgatatgtt accagcagaa agcaaataag ggggtatttg attatgagat taacaaatgg
     6961 aattgtatta gataacgaaa aaacttttgg agtattgaaa ttctctgcac ttcgacatga
     7021 ggtgcgtgta gaaaatgagg acggaacgac aagtgatgaa attaagcgtc gtacttatga
     7081 tttgaaatgt agtgtgcagg aacgcatgat acaagtgtca atccccgctg atgttcccgt
     7141 aaaggacttt ccttataatg cggaagtgga gttaatcaat cccgtagctg atacggtggc
     7201 aaatgccaac tttagaggga ctacggttga ttggtacatc aaagctgatg atattgtatt
     7261 gaagaacaaa ggcggtcaag ctggaaatcc gcagaacaac gctccgcagg gacagcagaa
     7321 aaagtaagct gacaaactag tttttcattg taggagatat aaacaactgt tataatatgg
     7381 atgtggaaac agttaattta gacttataaa ggagaaatgt gtatgctaga taaaatacct
     7441 aatgcagagg aaatgactgc tttggtcgga caatcattat atagtgtatg gaatgaattg
     7501 tgtgccttga ttgatgaaaa gtatgaaatg gaatgtctat ggaataaagg tggcaaagca
     7561 tggaattatg aatataagta tcgtcgaggt ggcaaaacat tatgtgcctt atatgctaga
     7621 gaaaattgtg tgggatttat gattatctta ggtaaagatg aacggttaaa attcgataca
     7681 tatagagata attattcaaa agaagttcag agaatttatg atgaaacaaa aacttaccat
     7741 gacggaaaat ggctgatgtt tgagccaaca gatacaactt tgtttgacga ttttgttaaa
     7801 cttttgagga taaaaagaaa accaaataga aagtagttta ttaccacacc attagcaaat
     7861 ggatagtttc ttataatagc ggatatatga aaatgctgtc atgtggacat aaagaaaatt
     7921 ggaagttgtg gaggtagcaa aaatgaaatt aaaaaaccct atgttggtag taactgatat
     7981 agataaatca gttgagtttt ataaaaaggt ttttggactt tatgtcatta tggattttgg
     8041 ggcaaataaa actttaacag gtggattagc cttacagaca agtgaaacat acaaagagtt
     8101 tatcggtaca agtgatattt cttttggtgg taacaatttt gaagtctatt ttgaagaaga
     8161 agattttgat aaatttgctg ataggctgaa agaatatgat attgaatatg tccaccctat
     8221 tatagaacat tcatggggac agcgtgtagt ccgcttttat gacccagata aacatattat
     8281 cgaagttgga gaaaacatga agatagtgtg caagcgtttc ttaaatagtg gaatgacacc
     8341 agaacaagtt gcagaacgta tggatgtacc tatgaaattt ataaatgctt gtatgcgata
     8401 aattgaaagt taggagacta atactatgaa aggatttgaa cgcaaaaatc aattattttc
     8461 tctttgcgga ttgaattgcg gactatgccc tatgcttttg ggaaattact gtggcggttg
     8521 tggcaatggt aatcaatcat gcagaattgc aaaatgtagt cttgaacatg gacagattga
     8581 atattgctat gaatgcaaac aatatccatg cgaaaaatat caacacattg atgattatga
     8641 ttctttcatc acgcataaac ggcgaaaagc agatttagaa agagcaaaaa acataggcat
     8701 tgagcaatat aatcttgaac agcaggaaaa aacacaaatt ctttcttact tactttctaa
     8761 ctataatgac gggcgtagaa agactttctt ttgtgtggca gtcaatttat tagaactttc
     8821 agaattacaa gaagcaatga aacaaataca atccaatgac gaattgacct tactaccatt
     8881 taaggcacaa tgcttgtatg tggtggaggt gtttcagaag attgctgaaa ggagaaatat
     8941 aaaactgaaa cttttcaaga aaaagtgatt ttcaaatttg aaattgtaac aaagattatt
     9001 tatatgacga atgggggaat acctttggaa acagtagagt taagatttca atatactcaa
     9061 agtgaatata taaaagcaga aaggcaatat ttaatttcta gtaaaactat acataaatat
     9121 gatattgctt tagtcgctgt atttttgttg ctgtcagtcg tttatatgct gttctcctct
     9181 tttagcgtat ttagtatttt aatatttgga ttgataattg ttgtaactgc attgggaagc
     9241 tatttatata tcctaatgcc tatactgaaa ttcaaacaaa ctgcaaaata tcatgaggaa
     9301 tatacgctgg tattttctaa ggaaaacatt aaatttaaaa cacaaagcat tgactcagaa
     9361 gtgaagtggg acatttactc tgcactatgg gaaagccatg atttttatta cttaattcaa
     9421 gcaccacgat tatatacgtt aattcccaaa agaattttta aagacctaaa cgaaaaacag
     9481 ctatttgaag aaattgcaca atcaagagtt aaaacaacaa agcatatata atgaacatac
     9541 cctacaatgt aacaacttca tattcataca cacaaagcgg ttagtctttt aagattgact
     9601 gcttttttca tacttaaatt aagattggag tgattgattt atgcgaatga ttttgaataa
     9661 aggacaccgt atcagagcca gagacaagaa ccttgtctac cacttttcca tagggacgct
     9721 tctgtttgta tttgtggcgg ttctcctgct actgaatatc aaacagctca tgcgtaccga
     9781 ctgggaacat ttcagcttat tggacaatgg cttgacgctt tccccctaca acttcataac
     9841 catattgata gcgactggta tttgtgcatt ggtcgctttt ctgtattacc gcttctgtta
     9901 tgacagtttc aaaaagctcc tgcaccgtca aaagctggca cgaatgatac tggaaaacaa
     9961 gtggtatgaa gcagataccg tacaagacag cggttttttt actgacctgc aaagcagatc
    10021 aagggaaaaa atcgtctggt ttccaaagat ttattatcaa atggaaaacg gattgcttca
    10081 tattcactgt gaaatcacat taggaaaata tcaagaccag cttttgcgat tagaggataa
    10141 gctggaaagc ggtttgtact gtgagctgac cgacaagacc ttgcatgacg gctatattga
    10201 gtacacgctt ttatatgaca tgatagcgaa ccgtatttcc attgacgaag tgaaagcaga
    10261 aaacggctgt cttaggctga tgaaaaatct tgtctgggaa tatgacgtac tccctcacgc
    10321 tctgatagct ggtgggactg gtggcggtaa gacctacttt ctgctgacgc ttattgaagc
    10381 tctgctacat acgaatgctg tcctttacat cttagaccca aagaacgctg accttgcaga
    10441 cttaggtaca gttatgggaa atgtgtatca taccaaagaa gatatgattg attgtgtcaa
    10501 tgccttttat gagggcatgg tacaaagaag cgaagaaatg aagcaacacc cagactataa
    10561 aacgggcgaa aactacgcct atcttggttt accgccctgc tttcttatct ttgatgaata
    10621 tgtggcgttt tttgaaatgc tggggacaaa ggaaagcgta agtttactta gccagttaaa
    10681 gaaaatcgtt atgttgggac gacaagctgg atatttcctt attgttgcct gccagcgtcc
    10741 agacgcaaaa tatttctcgg acggtatcag agataatttc aatttccgtg ttggattggg
    10801 gcgtatcagc gaattgggtt acggtatgct ctttggttct gatgtgaaaa aacagttttt
    10861 ccagaagcgt atcaaggggc gtggctattg tgatgtggga acaagtgtca taagtgagtt
    10921 ttacacgcct ttagtcccga aaggacatga ttttttgcag accataggct ctcttgcatt
    10981 cgtaaggcag gacgggacgg cgacgtgcga agcgaaaggc gacggcacgg actagccgtt
    11041 gctggtgtgg cgttagccac gccagcagat ataagcccct cattatctaa cagaggggca
    11101 caattacgct ggaaactggt tcaaaaacct tgaaaagtcc gctaggaata aggattttct
    11161 tagtttttag ctagttgtga taatcacttg aaaatatcac aaaatcacaa caatatcacg
    11221 attgggggta tgacaactga atgatactga ttttctgaac accttgaaag caaaacgcag
    11281 tgattatggc gtatcgcaga caaggcttgc aatcatggct ggtatcagcc gtgaacatct
    11341 aagccgtatc gaagctggga aggtagcact gaccgaggat atgaagcaca aactactgga
    11401 agcggtggaa aaattcaatc ccgacgctcc catgttcctg ctctttgatt atgtgcgtat
    11461 ccgatttccc acgctggata tacagcacgt tatcaaggac attctaaagc tgaatataga
    11521 ttatatgctt cacgaagatt tcgggcatta caagtacacc gagcattact atatcgggga
    11581 cgtgttcgtt tacacttcac aagacaagga aaaaggtgtc ctgctggaac tgaaaggaaa
    11641 aggctgtcgt cagtttgaaa gctatctgct ggcacaggaa agaagctggt atgacttcct
    11701 catggacgca ttagttgatg gcggtgtgat gaagcgtatc gacctagcta tcaacgacag
    11761 aacgggctta ctggatattc cagaactgat agagaaatgc aagaacgagg aatgtatttc
    11821 taaattccgt agcttcaaga actatggttc tggggagctg gtaaagcaca acgaagccaa
    11881 caaggacggc atgggacata cgctttacat tggttcattt accagtgaag tgtacttctg
    11941 tgtctatgaa aagaattatg aacaatcctc aaaattaggg ataccgattg aggaagtccc
    12001 cattaaaaac cgatttgaga tacgtttgaa aaatgagcac gcctactatg ccattagaga
    12061 gctgctgacc cactacgacg cagagctgac ggcattttct attatcaacc agtatatccg
    12121 ctttgccgat aaggaagcca acaagcgaaa aagcgactgg aaaaccaacg cccgctggtc
    12181 ttggttcatt ggcgagggac gaccgcccgt gaagctgacg acaaagccag agccttacac
    12241 tctgcaaagg acgctgaact ggttacaaag acaggttgca ccgaccttga aaatgctgaa
    12301 acagatagac gctggcaatc agatggacta tctcaagacc attgaacagc aggcaaagct
    12361 gacagataag cacaagcaga taatcagaca gcagacggct tcggtggacg aactgataga
    12421 aaaagaaaag gagtgattga tgatgtggga attgattaag gacttactgc tggtatcact
    12481 tggcatgggt atcggcgtgg ttctgatgtg tattatgagt gtcggaaagg aagctgaccg
    12541 acacatgatt gaattaaaag aaagcgagga aaaataaatg aatttcgggc aaaatttata
    12601 tacatggttt ttaagcaacg cccagagctt agtgcttatg gcgattgtgg ttattggtat
    12661 ctacttaggg tttaaacgtg agttttcaaa acttatcggc ttcttggtgg ttgccttagt
    12721 tgctgttgga ttggtattca atgcaggcgg tgtcaaagac gtactcttag aactattcaa
    12781 taagattatc ggcgcatagc cgtacttttt cattatggtt tgcggatacg caaacatacc
    12841 tttcccactt tgggagtggg ctaaggggaa agggggtaac ggaatggtac acagataaaa
    12901 atacagcata atgaatttca ttgcttggct caaacatagt tgatataata aagatatgaa
    12961 aataataaac gaaagttttt ttgataagga gtgatattgt gaaattgtgt attgttttat
    13021 ctccatataa ggcttaatgg aatagacttg tatggagtga aatgttatcc agagagcctt
    13081 acattttatc aaatttcaaa atgtaaagga gaatgggcat ggaatataaa tatacaaaaa
    13141 ttgatttgaa taaatggaat agaggtaaat tgtttcaaag ctacattgat aatatgcgta
    13201 ttgttatgag cttaaccgta gatattgatg taacaaagtt tattgaattt tcaaagaagc
    13261 atgatttaaa attttatcca gctatgatat gggctgtttc aaaggttgtt aatgaacatg
    13321 atgagttcaa atatagttgg gataataatg ggaatttaat taagtgggat tatatttctc
    13381 cgtcctatac tgaatttcat aaagaagatg aaaatttcac aaagatagtt acagattttt
    13441 ctgatagcat atttgaattt cataaacgtt ttatattaga tagggagaag tataaaacag
    13501 aacgtgcatt tgtggaaaat caatctttga acttttttga tgtatcttgt ttaccgtggg
    13561 taaaatataa tcattttgat gttcatgttt ttgacgaggg aaaatttctt gcaccagtta
    13621 ttacttgggg caaatatgaa ttagagcaag aaagatatat aatgcctctt acaatgaata
    13681 tacatcatgc cgtagcagat ggattccatt tatccagatt ttttaatgaa gtacaagaat
    13741 tgataaactc aatttaacag ctatatacaa agcagttagt ctatacgatt gactgctttt
    13801 tttcatacct aaatttaaga ttggagtgat tgcttgaaag atatattttc ggatatgcag
    13861 gcaagtatcg gctgtaccta catttcagat ttaccacatc ataaacgtat ggtgtggcag
    13921 gaaatgaagc gactgaacct tgccgattat ccgcagaaac aattagaaga ttttgcaggc
    13981 tatgtctttg gtgtccctta cactagcata gctgaagcat gaaagaaagg acgtgaaaac
    14041 ctatgaatga aatgcgtgtc tgtatcgaaa gccgactaca cacttctgaa gaaatccgtt
    14101 ccgcatggtt cacacttccc attgacgagg aagaactgga agaacagata ggcgttccta
    14161 tcgacagtga agattaccat atcgttgaaa aggaactgcc ctttgctgat gaagtcggcg
    14221 aagatgcgac cattgaaaaa ctgaatgacc tttaccatac ctttgaaagt ctgcctgccg
    14281 atttacaaga ggactacggg gagctgatgt gttactttac cagccttgac gaactgcacc
    14341 agcaccgcca tgatgtgata cattattcat ggtgtaaaaa catggctgat gtggcaaggc
    14401 atttgttgga caatgaccct gctttttctg ccatcgagga acgcctgacc cgttactttg
    14461 attatgaagc ctacggacag tatcttgatg ataacggaga atttatagaa acagaccacg
    14521 gtatctatga actgccgtag aagtgaggtg tttctatggt agaggatatg cgtgtatata
    14581 tcgctaatct tgggaaatac aatgaggggt atctggtcgg cgactggttc tcttttccga
    14641 ttgacgaaga agatgttgcc gagcgtatcg gcttgaatga acggtacgaa gaatacgccg
    14701 tacatgatac ggaaaacttt cccattgaga ttggcgaata tatctcgatt gaagagctca
    14761 atgaaatgta tgaaatgata tgtgagcttc ccgactacat cacggacagt ttggacgaat
    14821 ttgtatcaaa ttatggaagt ttggaggaac tgtacgacca tagggacgat atgattttat
    14881 acgctggctg tgaggatatg accgacgttg cctattactt cattgatgaa ttgcaggcac
    14941 ttggcgatat tccccctagt ttgcagaact atattgacta tgaagcatac ggcagggatt
    15001 tatcctttga gggaactttt attgaaacaa gatacggtat gtgtgagata ccgtggtaag
    15061 aagctggaag atcagcgaca agcattgttg cttctgacag ctttttctat tggggacagt
    15121 ctgaaaaacg gctgtccttt tctattatac gaaaggagtt gaaacaaaaa cgtgaaaaag
    15181 attaaaagct acacgggtat ctggaacgtg gaaaaagtct tgtatgcaat caatgacttt
    15241 aacctgccct ttcctgttac ctttacacag attacatggt ttgtgataac cgagtttatc
    15301 atcattctgt ttggggactt accgccctta tccatgattg agggggcatt tttaaaatac
    15361 ttcggtattc ccgtagctct tacatggttc atgtcgcaaa agacctttga cggcaagaag
    15421 ccgtacagct ttttgaaatc acagataacc tttgccctac gaccaaagat tacttatgca
    15481 ggaaaagccg tgaaactgga taagcaggta ctacatgaag ctattacggc agtaaggagt
    15541 gtgagctatg ttcccgatta aatatatcga caacaacctt gtctggaaca aggacaatga
    15601 ggtgttcgcc tactatgagc tgatacctta caattacagt tttctatccg ctgaacagaa
    15661 attcattgtg catgacagct tccgacagct aatcgcacaa tcccgtgagg gcaagataca
    15721 cgctttgcag attgccacag aaagctcaag ccgaagcata caagaacagt caaagaagct
    15781 ggttacggga cgactaaaag acgttgccta tcagaagata gacgaacaga ccgaagcgtt
    15841 agtatctatg attggggaca atcaagtgga ctaccgcttt tttcttggct tcaagctcat
    15901 ggtaacagac cagagcatga acctaaagag cgtgaagcaa tcggcgtttc tcacattcaa
    15961 ggagtttatc catgaagtga accacacgct gatgaatgac tttgtttcca tgccaaatga
    16021 tgaaatcaac cgctacatga aaatggaaaa gctgttggaa aataaaataa gccgacgctt
    16081 caaagtaaga cgcttagata aaaatgactt cggctatctg attgaacaca tctacggcag
    16141 ggacggtgtc gcctatgaag attatgagta tagcttaccg aagaagaaac tgaaaaagga
    16201 aacgctgata acgcattatg acttgatacg tccgacccgt tgccttgtgg aagaaagcca
    16261 gcgacattta cgcttggaac atgaggacag cgaaagctac gtttcctact ttactgtcaa
    16321 tgcgattgtc ggcgagcttg attttccgtc cagtgaaatc ttctatttcc agcaacagca
    16381 gtttactttt cccgttgata ccagcatgaa tgtagaaatc gtggaaaacc gaaaagcgtt
    16441 atcgacggtt cgcaacaaga aaaaagagct gaaagattta gacaatcatg cataccagtc
    16501 tggcagtgaa accagctcaa atgtggtaga cgcattagac agtgtggacg agctggaaac
    16561 ggatttagac cagtccagag aaagtatgta taagctctct tatgttatcc gtgtatctgc
    16621 tccagacctt gacgagttga aaagacgctg tgatgaagtc aaagacttct acgacgattt
    16681 gaacgtcaag ctggtacgcc ctgctggcga tatgctgggg cttcactctg aatttcttcc
    16741 tgccagcaag cgatacatca acgattatgt gcagtatgtc aaaagtgatt ttctagctgg
    16801 gctgggcttt ggtgcgacac agcagttagg ggaaaacacg ggtatctata tcggctattc
    16861 tgtcgatacg ggacgcaatg tttatctaca accgtccctt gccagtcaag gcgtaaaaga
    16921 tacggttact aatgctcttg cttctgcctt tgtagaaagt cttggcggtg gaaaatcatt
    16981 ctgtaacaac ctgcttgtct actactctgt gctgtttggc ggtcaagcgg taatcttaga
    17041 cccaaaatcc gagcgtggca actggaaaga aacgctccca gaaatcgccc atgaaatcaa
    17101 tatcgtgaac cttaccagcg acagagaaaa tgcagggctt cttgacccgt tcgtgattat
    17161 gcagaatgtg aaagacgctg aaagtctggc aatcgacata ctcacattcc ttacgggcat
    17221 ttcttcaagg gacggcgaga agttccccgt acttagaaag gcagtgcgtg ccgttaccca
    17281 gagcgaccag cgaggattgc tccatgtgat tgaggaatta agaaaagaag atacacctat
    17341 ctcttgcaat attgccgacc atatcgacag cttcacggac tacgactttg cacatctgct
    17401 gttctcggac ggtacggtgc aaaatgcaat cagcttagac aaccagctca atatcattca
    17461 agtagccgac ttagtgctac ccgataagga cacgaccttt gaattactgt ccgtttccat
    17521 gctgattgtg attagtacct ttgcgttaga ttttatccac tcggatagaa gtgttttcaa
    17581 gattgtagac cttgacgaag catgggcgtt cttaaatgtg gcacaaggcg aaacgctctc
    17641 taacaagctg gttcgtgctg gacgtgccat gaacgcaggc gtttattttg ttacacagtc
    17701 ctctggcgac gtgtcaaagg aaagtctgaa aaacaatatc ggtttgaaat ttgcctttag
    17761 aagcaccgat attaacgaaa taaaacagac ccttgaattt ttcagcattg acaaagagga
    17821 cgagaacaac cagaagcgat tgcgtgattt ggaaaacggg caatgcttgc ttcaagattt
    17881 atacggacgt gtgggcgtgg tgcagataca cccagtcttt gaagaattac tgcacgcctt
    17941 tgataccaga ccgccgatac agcgaaatga ggtggagtga tgaaagaaag gataaaaagt
    18001 gtgttcacaa gaaaaaagat attccgcttt ctcaaaatgg ctctgtttgt cgtggcactt
    18061 tcccttatcc tgctttcact tttgggaacg gtggcacacg caacgggact tgtggacgac
    18121 actatcaaca ccgagaacct ttattccaaa tatccgctgt cgaattatca gcttgatttc
    18181 tatgcagata ataactgggc gtggttgccg tggaactggc tggacgggat tggaaaaagt
    18241 gtgcaatatg ggctttactg tattaccaac tttgtctgga cgataagact atatctaagc
    18301 aatgcaacgg gctatgtagt gcaggaagcc tacaagctgg actttatcaa cgatatggca
    18361 gacagtatcg gaagaagcat acagaccctt gcaggcgtaa ctcaaaatgg tttttctact
    18421 tcgggctttt atgtgggatt tcttctgctg attatcttag tcgtaggtat ctatgttgcc
    18481 tatacgggac tgataaaacg ggaaaccagt aaggcactac acgccgttat caactttgtt
    18541 gtggtgttcg tattatcggc ttcttttatc gcctatgctc ctgactacat caagaaagtg
    18601 aatgactttt catcagacat cagtaccgct tcactggact tgggaacaaa aatcatgctt
    18661 cccgacagcg acagcaaggg caaggacagc gtggacttga taagggatag cctattttcc
    18721 atacaagtac aacagccgtg gctactgctt caatttggta attcgggtat agaggaaatc
    18781 ggggcagacc gtgtcgaagc tctcgtatcg gcaagcccag aggacgagga cggcaagacc
    18841 agagaggaag tcgtgaaaac agaaatcgag gacaacgaca acaacaatct gacgatactg
    18901 caggtggtaa accgtttagg tatggtgttc ttcctcttgt tcttcaactt gggtatcacg
    18961 atatttgtat tcttgcttac gggtatgatg ttgttcagcc agatactttt tatcattttc
    19021 gctatgtttc tgcctatcag ctttctatta agtatgatac cgagctatga gagcatggca
    19081 aaacaagcca tagtccgtgt gtttaatacg attatgacaa gggcaggtat tacactgatt
    19141 gtcacggtag cctttagtat ttccagtatg ttctacaaca tatcgacgga ctacccgttc
    19201 tttatggtgg cgttcttgca gatagtgtat tttgcaggta tctacatgaa gctgggcgat
    19261 ttaatgagca tgttctcttt aaacgctggc gacagtcaaa gcatgggacg gcgaatattc
    19321 cgcagaccgt atctgttcat gcgacatagg gctagacgta tggaacatag gcttgcaaga
    19381 gctgttagtg ctggtggtgt cgctggtgcg gtggctggtg gtgtcgttgc cagttcaaaa
    19441 tctacacgcc agaatggaac aaagaacaat cgtggaaata caagctccag catggggcag
    19501 aaagcaggtt cagccgtggg agctgtcctt gatacaaaga ataaggtcaa ggacaaggca
    19561 actgctgtca aagaaaatat caaagatatg ccgacacaga ctgcctatgc ggttcactct
    19621 gctaaggaaa aggcgaagtc cagtgtgtct gatttcaagc gtggcgtggt gcaggaaaag
    19681 gaaaaccgcc agtctggacg cttaaagaag caacagctac atagacagaa tatcgctgac
    19741 aagcgtatgg aacttcaaaa ggcacaagaa tcaaggcagg caggtaagaa aacggacgga
    19801 tcagcgacaa gcggagctac ccgtccccat gaaagaccag ctaccacttc taaggttaat
    19861 gtagaaaaac agcaggaagt caaacgccct gccatagctt caaccgtgaa gaatgatgaa
    19921 cctatcaaga aaaatgttgt caaggaacgt ccactatctt ctggcgatac atcagcaaag
    19981 accgtcaatc aagaaaccac aagacagaat tacaagactg accgtgttac gaaaacaacc
    20041 gttcagaagc atacacagca gaataccgag aagaacaaac gaaccgtatt caagaaagga
    20101 caaaagaaga aatgaaacta agacatatcg ctattgttgg cagtctgttt tctatcctct
    20161 tttctctcgt attattcttt ggggttctga ttagtgccga ggacgacgac gggggcaatg
    20221 gttattcctc taattatgta ggtatgaacc tatctgccga agtcttgaaa catcagccta
    20281 tggtagaaaa gtatgccaaa gaaaacggta tttccgagta tgtcaatgtg ttgctggtaa
    20341 tcatacaagt ggaaagtggc ggtacagccg aggacgttat gcaaagctct gaaagtctgg
    20401 gactaccacc caactcacta gatacggaaa gctcaatcaa gcaggggtgt aaatattttg
    20461 cgtccctgct ttcttcctgc aaaaatcaag gtatcagtga tataaatgtt gtgatacagt
    20521 cctataacta tggcggtggc tatgttggct atgtggcagg aaacggaaaa aagcatatgt
    20581 tcaaccttgc ggaaaacttc gcaagggata agtcgggcgg tcaaaaagta acctatacga
    20641 accctatcgc cgtggcaaaa aatggtggct ggcgttatgg ctatggaaat atgttctatg
    20701 tggagcttgt gaaccagtac cttgttgtca atcaagtatc gggcgagctg gcacaaaagg
    20761 ttatgaatga agccttaaaa tatcaaggtt ggaagtatgt atatggtggc agtaacccga
    20821 acacttcttt tgattgttcg ggactggtac agtggtgcta caacaaagct ggcgtatctt
    20881 taccgagaac cgcacaaaat cagtatgacg caacccagca tatcccacta tcgcaggcaa
    20941 aggcaggcga tttggtcttt ttccacagta cctacaatgc aggaagctat gtaacccacg
    21001 tcggtatcta tgtaggaaac actcagatgt atcatgcagg cgaccctatc ggatatgccg
    21061 atttaaacaa tgcttattgg caacagtact tgatttgtgc tggaagaata aaacagtaga
    21121 aaggattgga aaccatgttt aaaaagaaag aaaaaccagt aaaagaaacg aaagtccgca
    21181 ctatgaaagt aggcacacat aagaaatctg tgattgctct gtgggtggtg cttatcgcca
    21241 gcgtatcatt tggcgtgtat aagaacttta ccgccattga tatgcacacg gtacatgaaa
    21301 cggaaaccat tcagttaaga ctaaatgaca ccaacggagt tgaaaatttt gtgaagaact
    21361 tctgtaagtc ctactacaca tgggacaaca gcaaggaagc tatcgaagca aggacgcagg
    21421 caatcagcaa ctatctgaca aaggagttgc aggacttgaa ccttgatacc attaggacgg
    21481 atataccgac aagctccact gttacaaatg tactgatttg ggatataaaa cagtctggaa
    21541 cgaacgattt tatcgctacc tacgaagtag atcagcagat aaaagaggga gaacagacaa
    21601 gcaatgtcaa agcaacctat accgtcaaag tccatgtgga taaagacggc gatatggtta
    21661 tcgtacagaa cccaaccatt gcaccagcga ttgaaaaatc agactatgag ccaaaggcac
    21721 aagaagccga cgcaagcgtg gacgcagata ccgtcaatga cgctaccgca tttttggaaa
    21781 ctttctttaa gctgtatccg acagcaacag aaaaagaact tgcctactat gtggctggca
    21841 atgtgcttga acctatcggc agggactacc tctattccga attagtcaat cctatgttta
    21901 cggatgacag ggataatgtg aaagtcaggg ttgcggtgaa attcattgat aatcagacaa
    21961 aggcaatgca ggtatcacag tttgagcttg tgttacataa ggatagcaac tggaaaatta
    22021 ttatgtaaga tattttgttg gcaggctcta ttttcggata tagtctgcct tttctatagc
    22081 aaatgtgctg cactaacaag cgatttcatt gtattttaag caattaaatc tttcttgaca
    22141 attttatctt aaatcatata tttatggata ttgactttct gcttgcaata agttataata
    22201 tgactataaa actaaattag tcatatagga ggcgtgtggt atgtataaag aagttggtat
    22261 ttcttcaaat aatgttccga taacactttc tatttggtat acagaaagaa atagtcctac
    22321 cattgttttt ttgccgggta ctatgtcaca ccctctcatg tatgagaatt ttctatgtgg
    22381 tttgagtgaa agaggattta atataatagg cattcactat ctttcacacg gaaaaagccc
    22441 taaaatcaaa aaaaattata caatggaaga tttgctgcat aatgtatatg acgcaacgac
    22501 ttacggaata gaaaattttg gtgaaaacat agctgtcatg ggttccagtc aaggtgggat
    22561 tttagctgca atggcggcgg gtagggacac aaggttaaaa gccgtttttc cacataatat
    22621 catgctgact acattaaaag aaactatgag cttaacaaaa taccccgcat ggtcacaccg
    22681 ttttatgggg ctgatacagt tgggctttcg tgtaggtgga aaattgctgc ctaactataa
    22741 ggttccagga gacgcttatt tagactatga cagagtattt tatagtgaac aggcaaaaca
    22801 agattgctta aatgacccta tgcttttacc ctattatcct ttaagatttg tcgccagctt
    22861 atttaatgct gacttatcgt gcttggagaa tggggctata caatgtcctg ttattttaat
    22921 tactgccaaa ggcgatccgc tttttagatt tgattatacc aacaaagtat ttgatttaat
    22981 tcatgtaccg aaaaaggaat tacttacttt agagcttaat cgtcacatga tttttaatga
    23041 ggacacggac actacaataa acgcattgga agctaccttg cataaatatc ttggatagta
    23101 tattgattat ataatcagta aatgttatac ttttcatgac gataaaaaca ttttagtcat
    23161 aagggggtta tgagtttgcc acgatttacg gaacaagaaa aagaaatcat aaacagtaaa
    23221 cttttgatag agggagaaaa gctgttttca ttatacggct taaaaaaagt aacagttgat
    23281 gatttagcag ctgctgtaaa catatcaaaa ggttcattct atgcttttta tcccagtaaa
    23341 gaacatctat atgtggaaat caattttcgc ttacaaaaag agttatttac aagtatagaa
    23401 acaactgtta aaaagaaaaa gtataagaac catagagatt tagcaaagga cgttattatg
    23461 ctaagtttaa cgggggtgat tacttcgcct attctttcac agattgattt atctttaatg
    23521 gactatctgc aaagaaaaat atcgtcagat atttttgaaa atcatatgta cagtgatatt
    23581 cgtatattgg agatactgga aggtttaggg gttgtatttt tggttccgca tacggttatc
    23641 ataaaatccc tgtattctgt tttatcctgt ttggaacaat tcaaagagga cgaagaattg
    23701 aatatggtac aaaatctttt agtgaatggt attattcagc aagtagtagc agagtaataa
    23761 ttttgtttgg ttatttgaca ggcaagtggc caaacaaatt ttttacagtt tatatgacta
    23821 tatatctata aacgtcataa agttaaagga ggaaagaata atgattgatg taaaaaaact
    23881 gtattttagt tatactgaca aaccgtttgt tgagaatgta agtttccatg ttgggagagg
    23941 agaaattttc ggttttttag gaccctctgg tgctggaaaa tcaactattc aaaaaatttt
    24001 aactgggctt aatacaagtt ataaaggcag cgtaaaagtt gcaggtacag aaataaggga
    24061 gcatacaaac cggttttatg aaaatattgg agtagacttt gaattttcaa cctgctacga
    24121 gaaattcaca gcacgacaaa atctcgccta ttttgcttct ctatatgaca agcaacctcg
    24181 gtctattgat gaattattgc atatggtagg cttagaaaat gatggggaca aaaaagttgc
    24241 tgacttttcc aaaggtatgc gttctcgctt gaatttcatt aaagcgttag tacatgaccc
    24301 tgatatatta ttccttgatg aacctacaag cggtcttgac cctacaaata atcgtttaat
    24361 gaaagatatt attcttgcgg aaaagaaacg cggaaaaacg attatcatta ctactcacaa
    24421 tatgtatgac gcaacagagc tatgtgacca agtagccttt attgttgccg gaaaagtcag
    24481 tgcattggat agtccacata atttgattat gtcaagggga gctgccaaaa tacactatac
    24541 ctactatgat aaaggcgaga aaaccggaga atgtctgctt gatagaacca cagaagataa
    24601 gttgttaaaa agtttaattt ccgaaaaccg tctgctttct attcatagta gtgagcctac
    24661 attaaatgat atttttgttg atattacagg gaggacactt caatgagatt gggacgttta
    24721 atttgtggtg atatacattt ccaatggaaa tatggtttct attttatcta ctttatatta
    24781 acagttttat atgtgtgtgg gattgccgct ttgacgggac attggaaaac ggatattgct
    24841 tctatcatga tatactctga cccggcagct atgggattgt tttttatggg ggcgattgtc
    24901 cttttagaaa aaagccaaaa ggtattaaat gctatggtag tatcgcctgt aaaagtatcg
    24961 gagtatatac tttcaaaaac catcgcattg attgctattt ctacaatcat tgcggtgata
    25021 ttgggatttg tttccggcag caatcattta cttggtatcg cagttggtac agctttgaca
    25081 tcagcaatct ttactatgct tggcattatc gcggctacaa aaatttcaaa cttgaaccaa
    25141 tttctcattg tgattatgcc gatagaaatt gtttgctttg ttccacctat cgttggtctg
    25201 tttgtaaagt taccagatat attccgcttt tttccgttca cagcttgcat gaacctcata
    25261 actggaaaaa gcattttact atcgtttgat atggtacttg taattgctac gttgattatc
    25321 ctctacatag ttgcgcgaca tacagttaag catatgtgga gaagtttggg aggtgtaaag
    25381 ctatgaaaag gatattatcc agtaccatac aggtatttaa gcaaatcaag agcgacccta
    25441 tgatgtttgc agcttgtttt acgccttttg tcatgggagc tttgattaaa ttcggtattc
    25501 cctttcttga tggaataaca gatttttctt tgcaaacata ctatccgatc tttgatttat
    25561 tactttctat catggctcct gtattgctct gctttgcatt tgcaatgatt acattagagg
    25621 aaatcgacga taaagtgtca cggtactttt cgattacccc tcttggtaag tcggggtatc
    25681 tctttacaag gttgggagta ccctcaatta tttcggcggt catcgctttt attgtactgt
    25741 tgcttttttc attagaaaag ttgtccattg gaatgacaat atgtttggcg cttctcggct
    25801 cagtacaggc aattattgtt tcgcttatga ttataacatt atcaagcaat aaattagagg
    25861 gtatggcagt tacaaaacta gctgcactta cattactcgg aataccagct ccctttttta
    25921 ttgatagtta ctaccagttc gcggttggct tccttccatc attttgggta gcgaaagcca
    25981 tgcagaatga agcggttctt tatttctcca taggattgat ggtagctttg gtctggtact
    26041 acttccttat aaaacgtctg tttaggaagc tggcaggata agaaggcata ttatggaaaa
    26101 aggtagactt ctgcaaaacg aaaatataaa taaggctatt caggtttcca aaatcacact
    26161 aatcatcaac ctgggtttat cgctacttaa atttgctgcc ggatatatag gaaagtcaag
    26221 tgccatgctg tcggacgctg tacactcggc ttctgatgta ttaagcacaa tcgttgtgat
    26281 ggttggaatt aaaatatcag aaaaacagcc tgataaagaa catccttatg ggcatgaacg
    26341 tatggaatgt gtggcttcca ttattctctc tgttgcattg gctataacgg gtgctggaat
    26401 aggatattca ggtatcaaga aaatcttttc aggacagtac aatacgcttt ctgtctctag
    26461 tggaatcgca cttacggcgg cagtcttatc cattgttata aaagagtgga tgtactggta
    26521 tacaagaagt gctgcaaagc atacaaattc tgacgcactt atggctgacg catggcatca
    26581 ccgctctgat gcactctcgt cggttggttc cttaattggc attttaggag cacgtttagg
    26641 ctatgctatt ctcgacccta ttgcaagcat tgtgatttgc ggttgtatct taaaagctgc
    26701 tttggacatt ttcaaagaaa gcattaacaa aatggtagac catagttgtg acaatgctac
    26761 tgaaacgaag attagggaag ttgtgcttca gcagcaaggg gttgatggta ttgatgaatt
    26821 aaaaacccgc atgtttggag cgaaaatgta tgtagatatt gaaatattag ctgatggtaa
    26881 tttagctctt tatgacgctc atagaattgc tgagggagtc catcaaacga ttgaaaacaa
    26941 ctttccacaa tgtaaacatt gtatggtaca tgttaatacg aatgaacttc cctattctcc
    27001 ataggacagg gaagatgatt taagactatt gaaagggaaa ggaggggaag taggttatgg
    27061 aacaatactg gtggattatc attgttatgg ttttgattgt cgttggctta cttaaaattg
    27121 taattatgaa gaagtataac caaaggagaa attctaacaa tcgttcaaaa aatagcgagg
    27181 atgacaagat gtattaagag caattatccg atggtatgag atatatacct atgaaatttg
    27241 aacgataaat cccagtttgt aaaagtcaat aaatccaaag ataaatttgt atttgaaagg
    27301 agttttgaag aatgaaaaaa tatgagtatg tagaaattaa ttataatgcg aaaggagttg
    27361 tattcctttg tactgataaa cacaaagaaa ttattgacag ttatgcaaaa acggggtatc
    27421 actacgcagg gtttgtaccc acagaaattg acgcaaaagg ctgtatgcga aagatagatt
    27481 taatttttga aaaagaagat taacttattt tagggagttg gttctaatga taggacttaa
    27541 aagacgagat atgaattata ttatgaaagt tggtgctaaa gctcaaggtg tttctgtatc
    27601 tgaatttagg gctatgctac aagataccat agacaaaact atggaaagta cagacccaga
    27661 cgtgcaggca aattttaaac gatactttgg aaataagcgg ccaacaccag aggaatatat
    27721 ttatacgatt accaaaaaga caaaggttaa ggtttaaatg ttgtaaaggg ggcttgccgt
    27781 atgaaacgct ttaaatacaa ttcagtatat tcaagcccaa tttaacagca tactatttac
    27841 aattaaatac gctataccag tagctcgtac aaaatttata ttttgtgcgg gcttttttaa
    27901 tacccgaaaa gttttttaat tattttcatt tttttggaag caaatcacac cttgctgtac
    27961 agatatggtg cggaaagagg gataaacact tttcaattca tagctggaaa ggaggtgaga
    28021 ttatgaaacc gtctgacttc caaaaaacaa ttcagtgtca gtttgaatgt aagctgaaaa
    28081 aagttgtcaa aggtattatc tggaactacc gcaaagaatt aagacgaaga aaaaacaagg
    28141 aaatatcttt tagcgaatta ccagacatta cggtagaaag tctggcaaca tgggacgact
    28201 acgaaacaaa ctacacaatt tttaatgtgt gtggcataga tattaaagtc cttgatgatg
    28261 aacttgcaga agctctcaaa cagctacccg aaaaaaacgt aacaatgtat tgatgtatta
    28321 cttcttggaa atgagtgata cagagattgc agatattatg aattatacca gagaggctgt
    28381 gttcaaacac agacaccgtg cactagaaac tatgaaagag atactaaatg aaaagacaag
    28441 acagtaaagg acgcaatttg cgtcttggag agagccagag aaaagacgga agatacgttt
    28501 ataaatatac cgatatattc gggaagccac agtttgtata ttcttggaag cttgtaccca
    28561 cagacaagac acctgctgga aaacgtgatg atatatcgtt gagggaaaaa gaaaagcaga
    28621 taaaaaaaga ccttgacgac ggtatcgaca cggtgggcgg taaaatgaca gtctgccagc
    28681 tttacgaaaa aaagaacaat caaagaaaga acatcaagag aaacacggaa ttaggacgac
    28741 agtatcttgt gaacgcactg gaaaatgacc cattgggtat gagggcgatt gatacggtca
    28801 agcagtccga cgctaaagaa tgggctatca gaatgaatgg caaaggttat tcttacaaga
    28861 cgatagataa ctataagcgt tccctaaagg cttcattcta tatggcgata caagacgact
    28921 gtatcagaaa aaatcctttt aattccaagt tgagtgatgt tctggaagat gatacggaag
    28981 caaaggttat cctgacacca gagcaggaag aaaaactcct tgcctttatg gaaacagata
    29041 atgtttacag caagtatcgt gatgaagtta ttcttctgct ggaaacggga cttcgtatct
    29101 ctgaattatg cggactgaca acacacattg atatgcttaa ccgtgtgatt aacatagacc
    29161 accagctatt gcgtgataca gaggtgggct actatatttc caaagctaag acaaaaaacg
    29221 gtaagcgaga gcttccatta acagaaagag catatcaagc actcaagcga attttaaaga
    29281 accgaggaaa agcacaaccg cttgttgttg gtggttatag caatttctta ttcttgaacc
    29341 gtgagggatt gccaaaggta gcaggaaatt atgaaagtat ggtgcgtggg ttgattaaga
    29401 agtacaacaa gacccataag gacaagttac caaacatcac gccccattca ttcagacata
    29461 cttactgtac gaacatggca aataagggaa tgaacccaaa caccctgcaa tacatcatgg
    29521 gacacgcaaa cataaccatg acattagggt attatgctca cggtactttc cagtctgcaa
    29581 aggcagagtt agaaaggctg gcttcttaat atcgaaaatg tatcttacta cttatttact
    29641 acttttggtt gcattttcat gctggtaaat gccagcttat gcaaggtatc ttccaaaaag
    29701 aaaatgccca tacagcccga aaatgcggga tatatcggca tctaccaact tatgaaaaga
    29761 taatcagaaa tttagtaagt ttttataata aaaaaataag aggctgtcca aaaatagaga
    29821 taagcctttt ttcataatcg gaattataaa aatacacttg atttcatatg aaaaagagtg
    29881 tctcataaac taaaaagttt actttgagac actacctttt ttattcttat ttatactttg
    29941 tagacatatt ataatatgcg tttcactggt atctgaactt ctgtcaacca gtcttcttca
    30001 ctctctttat tccatatacc atctatataa gattctctag gatttccaac cactttatat
    30061 ttattgtcct ctatccattt aaatgcatct gcataagttt ttcctaatgt tgaatatgga
    30121 ccattgtgta atatacaaac tgcattttca actgcatcta attctttaaa ttttaatata
    30181 tccgtatctt cctttaaatc atttatagct tgacacattt caacatccat atcagtctct
    30241 ctatattcac catcatgata tatattaaaa caataatcag gaactgcaca tgtacaccct
    30301 gttttcatca tttcttcagc cattacattt ggaaatagat taaaaagttc atcatatccc
    30361 tttaacactt ttctcataga agcaacttta acttttggta aagacttaat aattacatca
    30421 ttcatattga cttctccttt caaattcata aggtaattac gtagtttaat tattttatat
    30481 tcttcacttt taattacatc caacatttct ttttctttat tcattaaaat attttcaact
    30541 tcttcaggat tttctacagc tttttttatc tcagctaatg ataaacccat ttgttttaaa
    30601 gttattatct gatgtaattt aggtaattgc tctgttgtat aatacctata ttttgtaaat
    30661 tcatctacat attctggttt taacaaccct atatcttcat aataccttaa tgtctttgta
    30721 gttattttat ttattttaga aaacattcct atagaaaaca tctcatcacc tgcgcattag
    30781 atttgattaa tttaagttta aactttcccc ttaggttaaa gtcaatacaa aataaattat
    30841 aattgtatta attcttcaat tctatctcca ctcaatacca aaatatcccc tgtttttata
    30901 atggttgaac ctttaggtat taaaacctgt tcctttcttt ttatcataac tattaatata
    30961 tcgtctggta tattagaatc tattatactc ttattagccc atttattacc ctcatcaaca
    31021 tttacttcga taagtttaga gctaatttcc ccatcataat cattaaatgt ttttaataca
    31081 ggtgtattat catcaactaa atccaacttt ctggctacag ttggtatcaa tgaaccttgt
    31141 attcctactg aaagcaacgc tataaaaaat attatatgga atatgtcatt ctctatggct
    31201 actccttgag taacagcata aattgcaaaa actactgaag ccgcacctct aagacctacc
    31261 cacgatatga ataatttttc tttatttgag aatttaaatt ttgctagaac tataaatgtt
    31321 gctaatggtc ttgcaataac tatcatgaat atagatatgg ccattgctat accaaataca
    31381 tttggtaact tagaagggaa agataacaat cctaacataa agaataacat tatttgcata
    31441 atccatgaaa ttccatcaaa gaaatgaacc aaactttttt taagaggtat cctactattt
    31501 cctataataa tacctgaaat gtaaacactt aaatatccat ttcctccaag atactcgcta
    31561 aatgcataag aaagtattgc aatagctgtt ataaatatag tataaaatcc ttctatctcg
    31621 aagtttgaat gccttaaaat ataaacagat aattttgcta gtataaatcc tatagctaac
    31681 ccaaaagcta tttgttttat taccattggt gcaataagtt ctaccccaga attattcatc
    31741 aatgtcaaaa gaattagtgt caacatatac gctattgggt cattacttcc actttcaact
    31801 tctaataatg atgcaagtga accccttaag tttagttttt gtgaacgcaa cacagcaaac
    31861 acagatgcag catctgttga agctgcaatt gccccaatta ataaaccttc aagtaaacta
    31921 gttcctatta ccatgtggca aaacatacct gtaaccccag acgtaattat tacacccagt
    31981 gaagacatta atatagaagg tatagcaaca ggtttagcca tattccagtt tgttccaaac
    32041 cctccaaaaa acattataaa tattagtcca atagaacata attgttttgt taattcatag
    32101 ttctgaacct cccatgacta aagtcacaga gttcctgctt catagaattt tgcatactaa
    32161 aatatgtctt actaattctc cacaggctat cccc
//
LOCUS       DAMJWP010000017        31660 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.018, whole genome shotgun sequence.
ACCESSION   DAMJWP010000017 DAMJWP010000000
VERSION     DAMJWP010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31660
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.018"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            109..426
                     /gene="flgB"
                     /locus_tag="PC700_000743"
     CDS             109..426
                     /gene="flgB"
                     /locus_tag="PC700_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HDF2580067.1"
                     /translation="MNTYDIIKMGLDATNLRSQTIANNIANINTEGYKRHYVTFEETL
                     NDAMPKIEEKTDNSKGLRVDGNNVDIEEEKVNQAMATLQYNALISFANGKIAMTKSII
                     SGR"
     gene            434..838
                     /gene="flgC"
                     /locus_tag="PC700_000744"
     CDS             434..838
                     /gene="flgC"
                     /locus_tag="PC700_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HDF2580068.1"
                     /translation="MSVFSGMRISASGLTAESLKMDIISSNVANINATRGPNGEAYRR
                     KVVSFEENYDNKLGMLGVKVTSVEDDKSPLKEVYDPSHPDAKPNGYVEYPNVNILVEM
                     TDLITASRAYDSNVDTLNAQKSMISKALEIGK"
     gene            848..1165
                     /gene="fliE"
                     /locus_tag="PC700_000745"
     CDS             848..1165
                     /gene="fliE"
                     /locus_tag="PC700_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HDF2580069.1"
                     /translation="MPITQINNLMQGAKLDNLIGETKPLTLNNKEEKKNFEDVIIDTI
                     DKVNFQQVTADDMKEKFIKGEDVSMHEVMLIGQEAQLSMQYLIEVRNKIHDAYQEMNK
                     VQI"
     gene            1190..2740
                     /gene="fliF"
                     /locus_tag="PC700_000746"
     CDS             1190..2740
                     /gene="fliF"
                     /locus_tag="PC700_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HDF2580070.1"
                     /translation="MIASFKNKIKEQVAKSRTKISSLKKGTKIALGIGVIAICLALVF
                     TAISASKNKYAVLFSELDVQDAADISAELESKKIDTKVKGSTIYVPRTEVDKLRLELA
                     PSLTGGSKGFALMDDSSSIGLTDEEFQIKKQRMIQGEIEKTIKSFPQVEDTRVHITFG
                     ESSVFEKEAIPGSAAVYLMLKPGTTLEKNQIKSVMALISGSVTNIPEKNVQVIDNKMN
                     LLSEGILEDEDVDENNQPTNSLINVDKNKASEQALNKQLEKSILEMLEPIFGKGKVKA
                     TVNADLSFDTAEKTEIKIDPDKVAIKETRSKNSSKSATNNVQGVDANMNNQGNNNGTN
                     TEDSLDESFEYETGKIETHTIVAPGELNRLTASVAIDGKIAKGVQADVEDIVNNAIGM
                     DGARGDTLSVVSMVFDPEGKAEAKQELEEIKKEGFKKNIIKAVIGVVAVVAIGAIGYL
                     IYKKRSEREDMDYEDEDYISKSDSVDRKDNPLLGDDPELTLEEAVKMYAEEKPDQVTE
                     IIKTWLNE"
     gene            2753..3823
                     /gene="fliG"
                     /locus_tag="PC700_000747"
     CDS             2753..3823
                     /gene="fliG"
                     /locus_tag="PC700_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HDF2580071.1"
                     /translation="MSTEITNVNAFDLSDINESDKKEKIEGEGLKKAAVLLMTLGPNV
                     SSDIIKSLPDKQVQKIGLEIANISSIASKERREILKEFVEINRAKDYVIEGGLEYARE
                     LLSNALGNAKANKLLEGISINTSTKPFKHIIKLDVKKILEILRDESAQTIAVLLAHME
                     PENGAEVLSYLDFDLQNEVAMRISSMGSISPEVIKVIESSLEDKLHSISGDDFKDYGG
                     VSTLVQILINSDRKTEKNILGTIEEQDPALAAQIKSHIFVFEDIVKLDDISIQKVLKE
                     VTMKDLVYALKGVDDEISDAIYRNQSSRAADALKEEMEMVGVIKVTQVEAAQQKIANI
                     VRKLEREGAITIVRNFGGDFVE"
     gene            3816..4547
                     /locus_tag="PC700_000748"
     CDS             3816..4547
                     /locus_tag="PC700_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HDF2580072.1"
                     /translation="MNNKIIKSKKASYKGVLDLTIIDKVRVGESNVNKENDCRFRDEV
                     IKEAIESAEIKKNEILEEAKMNAQNIEKQAYEKGYSQGQKNGYEDGYKESYDEYTQKA
                     KDEADFIKNQADLILLQANEKMTEYIKEKNKEIVRLAINMASSVLKKHFEDEESMSDL
                     LKQIIQDYEGSSSFIVRCNSFYKDNIEKEFELFKEETSLKSSVFVIADDSIDKGNAII
                     QKENGRIIVGIDCAMEKLKEELLGE"
     gene            4551..5870
                     /gene="fliI"
                     /locus_tag="PC700_000749"
     CDS             4551..5870
                     /gene="fliI"
                     /locus_tag="PC700_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HDF2580073.1"
                     /translation="MYKTDISIDFDKINKKIENLELTISEGRVKKIIGLTVEVEGIKA
                     FVGELCVIYNQVNKPVNCEVVGFKDKEVILMALGELTLIAPGCKVISKGIPLSVMCSD
                     NLLGKVLDGLGNPIDNSDAVLGDRYNLNNEPPDPMKRKKIRNIMETGVRAIDAFTTCG
                     EGQRIGIFAGSGVGKSTTLGMIARNAKADVNVIALIGERGREVLDFIDKDLGEEGMKK
                     SVVVCATSDKAPLVRLKGALTATAIAEYFRDQGKKVILMMDSVTRFAMAQREVGIAIG
                     EPPAQKGYTPSVFAILPKLMERTGTSDKGSITAFYTVLVDGDDFNEPIADTTRGILDG
                     HIVLSRDLANKNHYPSIDVLNSLSRLMNEIASKEDIKIASFARDMLAEYREAEDLINI
                     GAYASGTNKKIDEAIYYHEHIINFLKQGINEKSSFNETISSLRRIFE"
     gene            5902..6348
                     /gene="fliJ"
                     /locus_tag="PC700_000750"
     CDS             5902..6348
                     /gene="fliJ"
                     /locus_tag="PC700_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HDF2580074.1"
                     /translation="MELDKNFKFRLQKVLDLKIKDEEEIKMEFAKIQQKKIDIESNLE
                     NLESNYSKYSISKNNDSVQNQKITINYLLALNNSIMDLSEELDKSTNELEKARKQLIS
                     KQIERKSLEKLKEKKYGQYYKEEQLKEQSTNDEFASMSYLRNRQVL"
     gene            6376..7581
                     /locus_tag="PC700_000751"
     CDS             6376..7581
                     /locus_tag="PC700_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HDF2580075.1"
                     /translation="MKIQADSYNIIKDIGNRKSSSKNKNIKNNKFEDNLYKATDHKKV
                     KDRRETSKQNVSSDKRENSDNECHLDEDKSNHLEDENTYEKDCSNNNEALHQLLNLLK
                     DNYKKDKDINTILNNLKKLSISDELKQELKLNKNILNKIANQNLELNRASTNNPNDIE
                     NLDIMKLRELLENNASEDIEVISKTTENDTKSIDLYSFDSNRIDTLNSNKQKDDSSDI
                     LEKLAGVNGNKSGNFGQVINKSVDMNKIKNDNVNLKQIESNVMDDSIKAIKHMKTNDI
                     QELTIKLRPKELGDMNIQLLKDGESMRAVVTVFNKDVFDSINKNIADLKQHLELTNVN
                     IKDVSVQMHSDNTNTSDTFDKAFEQQNRQNNQENMSKQNTNDKRNNVVIEDEIKENII
                     DDDRVDLLA"
     gene            7599..8207
                     /locus_tag="PC700_000752"
     CDS             7599..8207
                     /locus_tag="PC700_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgD"
                     /protein_id="HDF2580076.1"
                     /translation="MYGIATALAGTQVNYRSNNLSQLERNINDIANKQVRSKGNQTVT
                     DNGTPIIGQGDDADKDLFMKILVAQMGNQDPMNPQDPTEYVSQLAQFATLEKMSAMND
                     KLDILLALSNSTLINSALSMATSLIGKEVEVSGEEEEDENIVGVVKSTYIKDGEVHLE
                     LEVDGSDKTVGVKYEKLLKIGEVDANSGEENDSSEQESPEEV"
     gene            8234..9217
                     /locus_tag="PC700_000753"
     CDS             8234..9217
                     /locus_tag="PC700_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HDF2580077.1"
                     /translation="MIKAMYSGVSGMKANQTKLDVIGNNVANAGTTSFKKSSARITDS
                     FYQTVLYASAPTAALGGTNLGQVGVGSKISSINKDMTQGNVQPTNRPSDLMIDGDGYL
                     PVVRQGTVMYTRDGSFNLDTGFKAADGALKNIKGGRLVNGDGYMLQGVIYDGEYKADT
                     GEFIKKGDAKGKEPIVIPLQSISPKDGTVQNVLEYNISKDGTVEFLLSDGQRTQFVYD
                     IPDGAKVGDTPAGIDKLKGTIQKVQTYAFQNPAGLDAEGGNLFKPSANSGDPQIAGAT
                     GNIIQGAIEASNVDIAEEFTEMIIASRSFQANSKIISTSDEILQEIINLKR"
     gene            9230..9433
                     /locus_tag="PC700_000754"
     CDS             9230..9433
                     /locus_tag="PC700_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HDF2580078.1"
                     /translation="MIRVTDLNGRRYILNSDLIVRIDSVPESRILLTTGEKNLVKESV
                     DEIVDKVIKYKRKVNVGYIRSEK"
     gene            9433..10251
                     /locus_tag="PC700_000755"
     CDS             9433..10251
                     /locus_tag="PC700_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HDF2580079.1"
                     /translation="MKKTDSLTFIGLILSTVLVLFGAVKGSSSGLKSFFDFASILITV
                     FGSFGALMITFTIDDIKLIKDALQYSFKTMNISKLDLLNQFKTLSKKARREGLLSIES
                     DVMKIQDPFMKKGLELCIDGLEVNEIRSILELEIDSVEETNNRYSKLFKTWGTFAPAF
                     GMLGTLIGLIQMLSDLTSPDLIASGMGKALITTFYGSLLANIALNPIAYNIDEKTEKE
                     IYVKEMMLEGIISIQSGESSIVVEERLATYLSNNEKLEIMKSNKNTERAMSNGA"
     gene            10241..10936
                     /locus_tag="PC700_000756"
     CDS             10241..10936
                     /locus_tag="PC700_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDF2580080.1"
                     /translation="MARKKNKKKDEINPDAWLATYADTITLILTFFVLLYATSVVDME
                     KFKNIADALKGQFSGISMFSTGSSTSEKSPIESVLVDKNDKSNLEQDLKDKVNNSNLQ
                     NSVTVKDDSRGILLELDDSILFDSGISELKADSKATLNKVYDMIKMMKNKIVIEGHTD
                     NVPVKSSTHESNWDLSSSRAVSVVRYFVEEKGMDPRLFSATGCGEYQPLVSNETAKGR
                     AENRRVNILITID"
     gene            10948..11391
                     /locus_tag="PC700_000757"
     CDS             10948..11391
                     /locus_tag="PC700_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HDF2580081.1"
                     /translation="MFKNKKIIIIILILIIFSAGAGFFILGEGGNKQIDKIAGLFIPT
                     GKGKDEVKTENLDLEEMVLKLADEGVRYLKVSITVSYDSSYKDIEKNTPLIRDNIINC
                     FMAKRADDFTADNLKNIKEEIKNTLNIQLGENVIQNIYFTNIVVQ"
     gene            11406..11780
                     /locus_tag="PC700_000758"
     CDS             11406..11780
                     /locus_tag="PC700_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliO/MopB family protein"
                     /protein_id="HDF2580082.1"
                     /translation="MILYLLKILICIPIILILIVLSLKISQGTLLKKYNDKYIKILDI
                     VNISKNNSIIILKIGEEGCIMASSSSSMNKIKDLTKEEILRIENDIKERNFKIQDFKM
                     ERIKKKYDFSRYIGKFTKKEDK"
     gene            11781..12446
                     /gene="fliP"
                     /locus_tag="PC700_000759"
     CDS             11781..12446
                     /gene="fliP"
                     /locus_tag="PC700_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425215.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HDF2580083.1"
                     /translation="MELLSKLPLADVPYTSSMQLFLFLTALMFLPFVMFMMTSFVRIV
                     ISLSFLKSALGAQQAIPSQILVGLAIVLTIFIMRPVLNEINEKALQPYMKEEVTMEEA
                     MKEAEGPIKEFLLKQTRQTDLDLFVEQAGLKEKKLTRENIPLSVVVPAFAISELKTAF
                     QIGFLIYIPFLIIDLVVASVLMSMGMFMLPPVMISLPFKLLLFVMVDGWNLIVKTLIL
                     GFG"
     gene            12459..12728
                     /gene="fliQ"
                     /locus_tag="PC700_000760"
     CDS             12459..12728
                     /gene="fliQ"
                     /locus_tag="PC700_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HDF2580084.1"
                     /translation="MSEFMIMSVLKNGMFTGAMIAAPVLIVSLVVGLLISVFQATTQI
                     QEQTLTFVPKLIAILLVVAIGGSWMLHTLISFTSEIFNMMSLITK"
     gene            12740..14554
                     /locus_tag="PC700_000761"
     CDS             12740..14554
                     /locus_tag="PC700_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused FliR family export protein/FlhB family
                     type III secretion system protein"
                     /protein_id="HDF2580085.1"
                     /translation="MIFVFIRIVAFFGTLRILFPAGTPALFKSLFAIIISILISSTMQ
                     IEYATNIDNIVLFTLYGINETITGIMLGYITNLCFYSIRMAGSMMDQQMGLSMINMFD
                     PNSMTQTTLIDNLMNWTARMIFFSMDGHHVLIRGIRYSFELIPIGKSFVDNNIDYIIN
                     IFVQCFLTGFKIAIPIVLCLLMADFILGLISRSVPQLNVMIIGMPLKILVGIALFIIS
                     IPLIVNQISHLLSQIPKMYEGTFALAPMFFMGSTDKTEEATPKKKGEQRKKGNIAKSK
                     ELPVAMTLLAFTLLVPTLFSYVVDTLKSSLNYFLSLDFYMNINYSSIEKLVIAGLMDF
                     FKIFLPIAIPFLVLGIVANLFQVGILFTGETLKPNLSKLNPVSGFKNMFSMRSLSTLI
                     KDTAIISILAYIGYTFFQDNYLDILKLGNIYLPTLMYTVKDLVYSILSKICVAMIVIA
                     VADYVYQRYSHKKQLRMTKQEVKDEYKNSEGDPEIKAKIKQKQRQISSQRTMQAVPSA
                     TVIVTNPTHLSIAVRYEKGKDQAPVVVAKGADYLAFKIREIAKGNDIPIIENKPIARL
                     LYKQVEIDQEIPEDMYQAFAEILVAVYKIKNRYKVLKR"
     gene            14563..16638
                     /gene="flhA"
                     /locus_tag="PC700_000762"
     CDS             14563..16638
                     /gene="flhA"
                     /locus_tag="PC700_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HDF2580086.1"
                     /translation="MNKFNLKENMDVIVAFGVVGIVLMMIVPLPTFILDILLAINISL
                     SVLILLMTLFTTNVLDLSIFPTVLLVTTLFRLGLNLSSTRLILTQGYAGEVIEAFGSF
                     VVGGNYVVGIIIFLIILIVQFVVITNGSGRVSEVTARFTLDAMPGKQMSIDADLNAGV
                     IDDKTARKRRKELQQEADFYGSMDGANKFVKGDAIAGLIVTAINIIGGIVIGAVMLNM
                     TLMGAAQTYVRLTIGDGLVSQIPALLISTSAGIIVTRASTDENFGGLVSKQLTAIPKA
                     VMMTGCVLIVLGMMPGLPKPAFFSLGIGAIAAGYFLGREKKDTLEDLEFEKPDMVNPD
                     KNEVEDVTNLINVESIEVEIGYGLISLADESAGGDLLQRIASIRRQCAIDMGIVVQPI
                     RIRDNLQLNPNQYNIKIKGNVVGGYEIMPNMVLCMDPMNQGFSIFGIKTIEPTFGIEA
                     LWIESDKVEDAEIKGYTVVDSSTILITHLLDIIKSNAHELLGRQEVKTIIDAARENYS
                     AVVDELIPDLMTLGEIQKVLKNLLREKVNIKDRVTILETLADQSRNTKDIELLTEYVR
                     IALARSICNNLVDDDKTIVVATLSPETEELVSNNLQRSVNGTYPAVDPENTNKIFESI
                     QEVMNNVYFNHNIPVIVVSPKIRAPFRKLVEIVYPNLTVLSLNEIPNDIKIKAEGVVS
                     IYDDEKVYS"
     gene            16613..17629
                     /gene="flhF"
                     /locus_tag="PC700_000763"
     CDS             16613..17629
                     /gene="flhF"
                     /locus_tag="PC700_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HDF2580087.1"
                     /translation="MMTKKYTANTIQDAMNLAKMELGDNITLIDKKEVRKSGIQGIFS
                     KKDIELTIGWEKRENVEQKDLKREIEQLKLIINNMGFDNKNDNDIDKICKNLLSLELN
                     EEIVESIRIDLQEMKFNGIDTSKNLVEILKKKIKIENQAINGKIALVGPPGVGKTTTI
                     AKLAAKLVFEENKKVGVITIDTYRIGAVEQLKIYTDIMNIPFKGVISPDEMELALDEM
                     KDCDVVLIDTTGRGYKNSMQILEIKNLIDKAETDNIHLVVNCTTRESDTKAIIDSYRN
                     VNFKSLIITKLDETITYGSIFNIMNYAQKPISYITTGQNVPDDIIKPNEDKIIRLLLG
                     VESI"
     gene            17626..18504
                     /locus_tag="PC700_000764"
     CDS             17626..18504
                     /locus_tag="PC700_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HDF2580088.1"
                     /translation="MIDQAQILRKMAMEKRGLDEFIVENENVPKIITIASGKGGVGKS
                     NLATNLSICLTKLNKKVLILDADIGMSNIDIIMGVNVKGTIIDVINGEKNIEDIISQT
                     KYGVNIISGGSALNHIEDFTEAQRNKFIHSIEQIHDVDFIIIDTGAGMSKSLLSFIYC
                     STEFFLITTPEPTSLTDAYSLLKAISNFGIKRSANIIINRVIDLEEAKSTYKRIKMVV
                     DKFLNLNLELYGYIIDDKKVSLCVKKQIPFILEYPTSIASKCIIAIAKRMVSQSREDI
                     VDNKSFLRKMFSIFKS"
     gene            18518..19219
                     /locus_tag="PC700_000765"
     CDS             18518..19219
                     /locus_tag="PC700_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HDF2580089.1"
                     /translation="MNREELIKENMPLVKSIARKFFIPGKGFEYQDLVNSGVIGLIDA
                     INKFDAEKGAKFSSYSYIKIKSAILDEIRNQSPISKHNLTKVNKYNRVVEKLQSKLLR
                     EPTSYEIAKELNVSEKELHDIESNIDMLNIVSLNYVIFEDTNETVQDVISDREEEAPE
                     NIIEEEEKLEILSKAISNLNEREKLILSLYYYEDLNLKEIGKVLGVSESRVSQLHRKS
                     IRNLRNKIKELKYSI"
     gene            19235..19657
                     /locus_tag="PC700_000766"
     CDS             19235..19657
                     /locus_tag="PC700_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HDF2580090.1"
                     /translation="MIQIILIVVSILIIVTIVINLHKESKNKDNRYFDKIFEQEYEAN
                     DRLIIAEKRRFFLDKVLAEIRSNNHIKVYENYNLIKANEERKENIFIESKKEVRQENE
                     LSLKVKHLISSGYDDVEICKMLDIGKGELSLIRSCYKI"
     gene            19642..19827
                     /locus_tag="PC700_000767"
     CDS             19642..19827
                     /locus_tag="PC700_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HDF2580091.1"
                     /translation="MLQNIRILLCDKKVFLGIGIGILISTLCIMLFSNNDMSKAEIEK
                     KARALGMKYTSEMKVLE"
     gene            19868..20641
                     /gene="flgG"
                     /locus_tag="PC700_000768"
     CDS             19868..20641
                     /gene="flgG"
                     /locus_tag="PC700_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HDF2580092.1"
                     /translation="MIRGLYTAVSAMITNQQKQNVVVNNLANMDTNGYKSKQLLTKSF
                     DELKLEGYNNYANGTKQKQIIGDISPGISMDETITNFNQGPIKTTDNKMDVAIQGKGF
                     FLVSDAAGNQFYTRNGNFRTDNQGDLITSEGYYVLGTNTATGAVGHINVGNQKFEVAK
                     DNTISLDGNAMYKFNIVDFNDYNNLKKEGDNLYSGGGAMKVNNALTIQSAIESSNVDM
                     VSEINNMMTISREYEANQKIIQAMDSKLAKIASEIGSVR"
     gene            20662..21423
                     /gene="flgG"
                     /locus_tag="PC700_000769"
     CDS             20662..21423
                     /gene="flgG"
                     /locus_tag="PC700_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HDF2580093.1"
                     /translation="MYTMFYTSKTALSANQSKLDIISNNIANSDSTGYKKINMEYSDL
                     VDEVLNRPSYPTNGNDISTGTGAKATAPMRIYEQGALAPTDSKSNLAIDGEGFFRVIR
                     KDGTYAYTRNGGFNIDGLGKIVDDNGNILDVQFDAGYNYNNTNIDSTNLTVSRNGELF
                     VDNKKIGKINLYQPIGTQNFISEGDSLFVATDAAQIKQVEKVDIVQGYRERSNVSLQE
                     EFVDLIATQRAFQMNSKGIKTADEMWQIANNLRAK"
     gene            21446..22327
                     /locus_tag="PC700_000770"
     CDS             21446..22327
                     /locus_tag="PC700_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HDF2580094.1"
                     /translation="MNDMKEYDFGKPQSYSRENLTCLNFLLAEFCKKYKNYIIYELKC
                     NSSMIVDSIDQVNYQTFLDRVTSSCVVVQNAMEPVMKNFSFRIDRSVADMWIDITSGG
                     TGVIKDNDRELTELDKKVLLHLMDDLVRNMHLFEGFENIQVLDTHTHINLPQLCSPTA
                     PVCVVDLKVLNDNEYIGSVSLCFPYNGLEALLESISVIEFFDNDVGDDSEEFTKKIYN
                     SVSNIELSVIAEIGKVSINVEDLLKLEVGDVIVTNKKINDYIDIFVENSKSYTATPGF
                     ISSKKGVKINDAVGKEV"
     gene            22330..22704
                     /locus_tag="PC700_000771"
     CDS             22330..22704
                     /locus_tag="PC700_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HDF2580095.1"
                     /translation="MDNSNLISDEEAKMLLNDKVESEVDGDDELALDVSEVNRNNIQR
                     ILDLKLELSVVIGRTKMTLRDILNLHKGSLIELNTLADQDVEILIDNKVLAYGKVVVV
                     DLNFGVKITSIVSEEDMVKSLV"
     gene            22736..24139
                     /locus_tag="PC700_000772"
     CDS             22736..24139
                     /locus_tag="PC700_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF2580096.1"
                     /translation="MVLILNLNDIKKDILKLNKKDSCSLYNESINNINNNCISTGLKF
                     LKQARDLNPDDVDILNLTGLVNLLKCNFDDAVESFYKSSLCEKTALCEKYINMLTSEE
                     FLIFFEKYNQLIRLINKGMYKEAIQGFKLITDQYCDLIEPYELLALLYDKEEEYIKFD
                     ECLEVLKIIDKENYLLNQENNTLDMYNSANIQTKSNVKVDDYKNEFLHKSSSNHKIND
                     YNNVTKEKSSSKNQKSSDSNKKKSIKKNKYLYIAIILGILLVGQSIYSSYKMNNLTDK
                     ISKNNETKNEESIDSNLTKKENNIEENEDMSKASNINKSNKNINKEADVKSKKNTVEN
                     EDDKDSDLFTEDELMSKANTLKSEKKTKSSIQLYKKVADIGENKGNTSEATYQVAILS
                     EKLKDYKTAEEYYKMYVENYSEKDAYFDESYYNLGMMYYNNGDLKNSKLTLKKLVNKV
                     PNSMYNNSKVKEILKEE"
     gene            24255..26192
                     /gene="htpG"
                     /locus_tag="PC700_000773"
     CDS             24255..26192
                     /gene="htpG"
                     /locus_tag="PC700_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HDF2580097.1"
                     /translation="MEFEKGSISIHTENIFPIIKKWLYSDKDIFIRELISNGCDAVSK
                     HKRLVSLGEISENKSSDYKITVSVNKGEGTLKFIDNGIGMTEEEIKKYINQVAFSGAE
                     DFFNKYKDKMEESNDIIGHFGLGFYSAFMVSKKVQIDTLSYTEGATPVRWISEGGTEY
                     EISESDARNDRGTTITLFIDDDSKEFLDEFTVRGIINKYCSFLPVEIYLEDVERLERE
                     AKEAEEKAKKQKEDGKEEIVDAKVIEPLNDTNPLWLKSPKDCTDEEYKEFYRKVFNVF
                     DEPLFWIHLNVDYPFNLKGILYFPKLKNEFELTEGKVKLYNNQVFVADNIKEVIPEFL
                     LLLKGVIDCPDLPLNVSRSFLQNDRDVSKISKHIIKKVADKLKSLCKNEREEYNKFWD
                     DIQIFIKYGCLKDESFYEKVKECILFKTIDDEYITIQDYLEKCKDKHENKVFYVSDKE
                     QQSQYIKLFKEYDLSAVVLNSSIDTHFISFMEYKENGVKFNRIDADLSDVLKDKNENK
                     DSEENKEEIAKIEGLFKEAVGERVKNYSVEGLKNEDTPAMVLVSEQSIRMAEMQSRFA
                     GMDLGMNFEEEKTLVINENSPIIKKLVSLKDDEEKKDKITLICNQIADLALLSNKELK
                     PDELDSFVQRSNKLMSLLIEL"
     gene            26580..27710
                     /locus_tag="PC700_000774"
     CDS             26580..27710
                     /locus_tag="PC700_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDF2580098.1"
                     /translation="MNFNYNLPVNILFGRGRINDVGKEVSKYGKKVLIVTGKNSTKKT
                     GLLDKTIDLLKDSKIEYEVFDRVEQNPLITTIYSGVEIIKSTGCDCVLGLGGGSIMDA
                     AKSIAFSYKNPGDLNDYIFGIRQGEEALPIILVPTTSGTGSEGNCFAVLTNSETKDKK
                     SIKKNSMYAKASIIDPELMVTMPKHIIASVGFDALAHNMEAYLSNGRNPLADVQAIYG
                     IELISENLIKVYNDVNDLEAWEKITLASTIGGMTIGTAGTALPHGMEHPVSGLRDVMH
                     GKGLAALTPTIIESSWDIDIERFGNISKVLGGTCAEDCSDAIRNFLKKIDLDIKLSDL
                     GIEKSDIDWLTDNCMKISVANIKRHPKYFNKEQIKEMYHKSL"
     gene            27937..28959
                     /gene="splB"
                     /locus_tag="PC700_000775"
     CDS             27937..28959
                     /gene="splB"
                     /locus_tag="PC700_000775"
                     /EC_number="4.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HDF2580099.1"
                     /translation="MFNPKRIIFEKGTLDTDVGKKIYNKFRDNKDVEIINISSNRIKE
                     HIPGENLYEQYRSGKQTMVVGIRKSLKFQTCKPSANYQLPLVSGCMGRCEYCYLNTQL
                     GDKPFIRVFVNVDEILEQTKKYIEDRKPQVTIFEGAATSDPVPVEPYTNSLKKTIEFF
                     GREEKGRFRFVTKYSDVDSLLDAEHNNHTEIRFSINTPRIINEHEHYTASANKRIESA
                     VKLARAGYQIGFIIAPVFLYEGWKEEYKNLIISIKEALPKDFDKKIIFEVISHRYTTK
                     AKNRILEIYPDTLLPMENEDRKFKYGQFGYGKYVYDKEQLDDMKEFFLKEINGVFKNS
                     LIKYII"
     gene            29141..30052
                     /locus_tag="PC700_000776"
     CDS             29141..30052
                     /locus_tag="PC700_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2580100.1"
                     /translation="MHAWESIQITLDLIEANLSEEISINELANKANLSPFYYQRLFKR
                     LVNKTVMEYIKLRRLARASEYLAEHKNRILDVALNFGFASHETFTRSFKKAYGLTPEK
                     YRANPVKLNHFIKPELILNYAMVDEGVPLIADDIVIEVNRKTLSNPRTFVGIEIEVPI
                     CQLVGGETTGIAIVEELWMKLGSQRHNIPNQIPGGNEFAALYMGNAKEGNCMYMAGVE
                     VESGTSVEGYNTFELPAKEYLVCGFEAENFNELVNSAVFKADKFMERWMKKHNLTTTD
                     FAIEMYYPTTPEAAYLEHWIVPVPIEQ"
     gene            30067..30483
                     /locus_tag="PC700_000777"
     CDS             30067..30483
                     /locus_tag="PC700_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HDF2580101.1"
                     /translation="MNWQDAFFQDTKPTFEQINEFINNPLWDKLNKTLVSTYNVEPKL
                     EYSKCSMQRGWNVKYKKRGKSLCTLYPQEGYFKALVIVSESNRVEVDLFINTCCDYIK
                     KIYNEVNFFNGSKWLMIQVDSLLVLNDMLELIKFRA"
     gene            complement(30577..30969)
                     /locus_tag="PC700_000778"
     CDS             complement(30577..30969)
                     /locus_tag="PC700_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2580102.1"
                     /translation="MSINTEKNITKNVEELTCPIRYALDIVGGKWKLPIICMLAVENP
                     IRYSSIKRKLDGITNTMLAQSLKDLESTGIVHRKQYNEIPPKVEYTLTSKGKSIVPIL
                     QQFANWGATNMQEKNTCGLSCKECRKIK"
     gene            31110..31505
                     /locus_tag="PC700_000779"
     CDS             31110..31505
                     /locus_tag="PC700_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDF2580103.1"
                     /translation="MKALEFLNKAGVYYLATVDENNQAHVRPLGFVMEYNGKLTFCTS
                     NQKDMFKQLIFNPKVELCCIDQNLNTLRILGEAVFVTSKETQAKALEIMPSLGQMYSV
                     GDGKFEIFSIDKAKVSCCSMSGKKIDVEL"
BASE COUNT        12970 a         3366 c         5854 g         9470 t
ORIGIN      
        1 gaaatgtaat taatttggat gaataaagaa gtcatttttt gtaatatagc aacttcattt
       61 tttattaata agcaaaataa ataaaatata ttttgaggag gagtgagtgt gaatacatat
      121 gacattataa aaatgggatt agatgcaact aatttaagaa gtcagacaat agcaaataat
      181 atagccaata taaatactga aggatataag agacattatg taacttttga agaaacatta
      241 aatgatgcta tgccaaaaat agaagaaaaa acagacaatt ctaaaggatt aagagtggat
      301 ggcaataatg tagatataga agaagaaaaa gtaaatcaag caatggcaac attacaatat
      361 aatgctttga taagttttgc aaatggaaaa atagcaatga ctaaatcaat aataagtggg
      421 aggtaattaa aaggtgagtg tgttcagtgg aatgagaata tctgctagtg gattaacagc
      481 agaaagcttg aaaatggata tcatatcgtc caatgtagct aatataaatg ccacaagagg
      541 tccaaatgga gaagcatata ggagaaaagt tgtttcattt gaagaaaact atgataataa
      601 attaggtatg ctaggagtaa aagttacatc tgttgaagat gataaatcac ctcttaaaga
      661 agtatatgac ccatctcatc cagatgcaaa gccaaatgga tatgtagaat atccaaatgt
      721 aaatatattg gtagaaatga cagatttaat aacagcttca cgagcatatg attctaacgt
      781 agatacattg aatgctcaga agtctatgat atcaaaagct ttggaaatag gaaagtaggg
      841 ataaattatg ccaattacac aaattaacaa tttaatgcaa ggtgcaaagt tagataattt
      901 gattggagaa acaaaaccat taactcttaa taataaagaa gaaaaaaaga actttgaaga
      961 tgtaataata gatactatag ataaagttaa ttttcaacaa gtgacagcag atgatatgaa
     1021 agaaaaattt ataaaaggcg aagatgtttc tatgcatgaa gttatgttaa taggtcaaga
     1081 agcacagcta tctatgcaat atttaataga agttagaaat aagattcatg atgcatatca
     1141 agaaatgaat aaggtacaaa tttagccttt tgaacaggag taataatcaa tgatagccag
     1201 ttttaaaaat aagattaaag agcaagttgc taaatctagg acaaagataa gttctctaaa
     1261 aaagggaact aaaatagcat taggtatagg ggttattgct atatgtttag cgctagtatt
     1321 tacagcaata tctgcgagca aaaataagta tgcagtctta ttttcagaac tagatgttca
     1381 agatgctgca gatatatcag cagaattaga gtctaaaaag atagatacta aggtaaaggg
     1441 aagtacgatt tatgtaccta gaacagaagt tgataagtta aggctggagc tagcaccaag
     1501 tcttacaggt ggttctaaag gatttgcact aatggatgat agttcatcaa taggacttac
     1561 tgatgaagag tttcagatta agaagcaaag aatgatacaa ggtgaaatag aaaaaacaat
     1621 aaagagtttt ccacaagtgg aagatactag agtacatata acatttggag agtcatcagt
     1681 atttgagaag gaggctattc cagggagtgc agcagtttat ttgatgttaa aaccagggac
     1741 gactttggaa aaaaaccaaa taaaatctgt tatggcactt atatcaggaa gtgttacaaa
     1801 tataccagag aaaaatgttc aagtcattga taacaaaatg aatttattat ctgaaggtat
     1861 tttagaggat gaagatgttg atgaaaataa tcaaccaaca aatagtttga ttaatgttga
     1921 taaaaataag gcatctgaac aagcattgaa taaacaatta gaaaagtcta ttttagaaat
     1981 gctagaacca atatttggta aaggaaaagt aaaagccaca gtaaatgctg atttaagttt
     2041 tgatacagct gaaaaaactg agataaaaat tgaccctgat aaagttgcaa taaaggagac
     2101 aaggtcgaaa aattcatcta aatctgctac aaataatgtt caaggtgtag atgctaacat
     2161 gaataaccaa ggaaataaca atggtactaa tacagaagat agtttagatg agagttttga
     2221 gtatgaaaca ggtaagattg aaactcacac tatagttgcg cctggagaac ttaatagatt
     2281 gacagcttct gttgcaatag atggaaaaat agctaaaggt gtacaagctg atgtagagga
     2341 tattgttaat aatgctatag gtatggatgg agcgagggga gatactctta gcgttgtttc
     2401 aatggtattt gacccagaag ggaaggcaga agctaaacaa gagctagaag agattaaaaa
     2461 agaaggattt aaaaagaaca taataaaagc agttattgga gtagtggcag tggtagcaat
     2521 tggagctatt ggatacttaa tatataagaa aagaagtgaa cgagaagata tggattatga
     2581 agatgaagat tatatttcta aatctgattc tgttgatagg aaggataatc ctctattagg
     2641 agatgaccca gaactgacat tagaggaagc tgttaaaatg tatgcagaag aaaagccaga
     2701 ccaagtaaca gagataataa aaacttggtt aaatgagtaa gggggataga aagtgtccac
     2761 ggaaattaca aatgttaatg cgtttgactt aagtgatata aatgaaagtg ataagaaaga
     2821 gaagatagaa ggtgagggtt taaaaaaagc tgctgtatta ttaatgacat taggaccaaa
     2881 tgtgtctagt gatataataa aaagcttgcc tgataaacaa gttcaaaaaa taggtttaga
     2941 aattgcaaat atttcttcaa tagcttcaaa agaaagaaga gaaatcttaa aagagtttgt
     3001 agagataaat agggcaaaag attatgttat agaaggtggg cttgaatatg caagagagtt
     3061 gttaagcaat gctttaggaa atgctaaagc aaacaaatta cttgaaggta tatcaataaa
     3121 tacaagtaca aagcctttta aacatattat aaaactagat gtaaagaaaa tattagaaat
     3181 acttagggat gaaagtgcac aaactatagc tgttttactt gcacatatgg agccagaaaa
     3241 tggagcagaa gttttatcat acttagattt tgatttacaa aatgaagtag ctatgagaat
     3301 aagctcaatg ggttcaatat ctccagaagt aataaaggta attgaaagtt cattagaaga
     3361 taaattacac tcaataagtg gtgatgattt taaggattat ggaggagtgt ctaccttagt
     3421 tcaaatattg attaattctg atagaaaaac agaaaagaat attttgggaa ctattgaaga
     3481 acaagacccg gctttagcag ctcaaattaa atctcatata tttgtctttg aagatattgt
     3541 caagttagat gatataagta tacaaaaagt attaaaagaa gtgactatga aagatttagt
     3601 atatgcctta aagggtgtgg atgatgagat ttcagatgct atatatagaa atcaatcttc
     3661 tagagcagca gatgcactta aagaagaaat ggaaatggtt ggtgtcataa aagttacaca
     3721 ggttgaggct gctcaacaaa agatagcaaa tatagtaaga aaacttgaaa gagaaggtgc
     3781 cataactatt gtaaggaatt ttggtggtga ctttgttgaa taataagatt attaaaagta
     3841 aaaaagcatc atataaagga gtgctagatt taaccataat agataaagtt agagtgggag
     3901 aatcaaatgt aaataaagaa aatgattgta gatttagaga tgaggtaata aaagaagcta
     3961 tagaatctgc tgagataaaa aagaatgaga tattagaaga agcaaaaatg aatgcacaaa
     4021 atatagaaaa acaagcatat gaaaaagggt atagtcaagg tcaaaaaaat ggatatgaag
     4081 atggatataa agagtcatat gatgaatata cccaaaaagc taaagatgaa gcagatttta
     4141 taaaaaatca ggcagactta atactattac aagctaatga gaagatgact gagtacataa
     4201 aagaaaaaaa taaagagatt gtaagacttg ctattaatat ggctagttct gttctaaaaa
     4261 agcatttcga agatgaagaa tctatgagtg atttgttaaa gcaaattatt caagattatg
     4321 aaggaagtag ttcatttata gtaagatgta attctttcta taaagataat atagagaaag
     4381 agtttgaatt attcaaagag gaaacatcac taaaatcaag tgtatttgtt attgcagatg
     4441 atagtataga taaaggcaat gctatcatcc aaaaggaaaa tggaagaata atagttggta
     4501 tcgattgtgc tatggaaaaa ctaaaagaag aattgcttgg tgaataaagt atgtataaaa
     4561 ctgatatatc tatagatttt gacaaaataa ataagaaaat agaaaattta gaactcacta
     4621 tatcagaagg tagagtaaag aagataatag gacttactgt agaagttgaa ggaataaaag
     4681 cttttgttgg ggagttatgt gttatataca atcaagtcaa taaaccagtg aattgtgaag
     4741 tagtgggttt taaagataaa gaagtcatat taatggcgtt gggagagcta acattgatag
     4801 ctcctggttg taaagttatt tctaagggaa tacctttaag tgttatgtgt agcgataatt
     4861 tactaggtaa agtacttgat ggtcttggaa atcctataga caactcagat gctgttttag
     4921 gagatagata caacttgaac aatgaacctc ctgacccaat gaagagaaaa aagatacgaa
     4981 atataatgga aacaggtgta agagcaatag atgcatttac tacttgtggt gaaggacaga
     5041 gaatagggat atttgcagga agtggagtag gtaagagtac gactctaggt atgattgcta
     5101 ggaatgcaaa agcagatgta aatgtaattg cccttattgg agaaagagga agagaggtac
     5161 tagattttat tgataaagat ttaggtgaag agggaatgaa aaaatctgtt gttgtatgtg
     5221 caacatcaga taaagctcct ttggttaggc taaaaggtgc tcttactgct actgcaatag
     5281 cagagtattt tagagaccag ggaaaaaaag taatacttat gatggattca gtaactagat
     5341 ttgctatggc acaaagagaa gttggtatag caataggaga accaccagct caaaaaggat
     5401 atacaccatc tgtatttgct attttaccaa aacttatgga gagaacaggg acatcagata
     5461 aaggttctat aactgcattt tatacagtat tagttgatgg agatgacttt aatgagccaa
     5521 tagcagatac aacaagaggg atacttgatg gtcatatagt tttatctaga gacttggcaa
     5581 ataagaatca ttatccttct atagatgtat tgaacagttt aagcagactt atgaatgaga
     5641 ttgcttcaaa agaagacata aaaatagcat cttttgcgag agatatgctt gctgagtata
     5701 gagaagcaga agatttgata aatataggtg catatgcttc aggtacaaac aaaaaaatag
     5761 atgaagcaat ctactatcat gagcatatta ttaatttttt aaaacaagga ataaatgaaa
     5821 agagttcttt taatgaaact ataagtagtt taagaagaat ctttgaataa acaatttaaa
     5881 aaaatatttg aataggttgt gatggaattg gataaaaatt ttaagtttag attacagaaa
     5941 gttttagact taaaaataaa agatgaagaa gagataaaaa tggaatttgc taagattcaa
     6001 caaaaaaaaa tagatataga gagcaattta gaaaacttag aaagtaatta cagcaaatat
     6061 tctataagta aaaataatga tagtgttcaa aatcaaaaaa taactattaa ctatttgtta
     6121 gctttaaaca actctataat ggatttatca gaagagctgg ataagtcgac aaatgaactt
     6181 gaaaaagcta gaaagcagct tataagcaaa cagatagaga gaaagtcatt agaaaaatta
     6241 aaagaaaaaa aatatggaca gtattataaa gaagaacagt taaaagaaca aagcactaat
     6301 gatgagtttg caagcatgag ttatttgaga aatagacaag ttctataaaa tagtaggaag
     6361 tgaggatatt attgtatgaa gatacaagca gattcttata atattattaa agatattggg
     6421 aatagaaaaa gctcatctaa gaataaaaat ataaaaaata ataaatttga agataatcta
     6481 tataaggcaa cagaccataa aaaggttaaa gatagaagag aaacaagtaa gcaaaatgtt
     6541 agttcagata aacgggaaaa tagtgataat gagtgtcatt tagatgaaga taaaagtaat
     6601 cacttagaag atgaaaatac atatgaaaaa gattgtagta ataataatga agctttacat
     6661 caattactga atcttttaaa agataattac aaaaaagata aagatataaa cactatattg
     6721 aataatctaa aaaaactaag tatatcagat gaattaaaac aagaacttaa gcttaataaa
     6781 aatatattaa ataagattgc aaatcaaaat ttagagttaa atagagctag tacaaataac
     6841 cctaatgata tagaaaattt agacataatg aagttaagag aacttttaga aaataatgct
     6901 agtgaagata ttgaagtaat tagtaaaact acagaaaatg atactaaaag catagacttg
     6961 tacagttttg acagcaatag aatagatact cttaactcaa ataaacagaa agatgatagt
     7021 tctgatattt tggaaaaact agcaggggta aatggaaata agagtggaaa ttttggacaa
     7081 gtaataaata aaagcgttga tatgaataaa attaaaaatg ataatgttaa tttaaaacag
     7141 atagaatcaa atgtaatgga tgattctata aaagctatta agcatatgaa aacaaatgat
     7201 atacaagaat tgacaattaa attaagacca aaagaattag gagatatgaa catacaattg
     7261 ttaaaagatg gtgaaagtat gagagctgtt gtgactgttt tcaacaaaga tgtctttgac
     7321 tctataaata aaaacatagc agatttaaag caacatcttg aacttacaaa tgtaaatata
     7381 aaagatgtat cagtgcaaat gcatagcgat aatacaaata catctgatac ctttgataaa
     7441 gcatttgaac aacagaatag acaaaataac caagaaaaca tgagtaagca aaatacaaat
     7501 gataagcgta acaatgtagt catagaagat gagattaaag aaaatattat tgatgatgat
     7561 agagtagatt tactagctta gtaaggggga agtattgcat gtacggaata gcaacggctt
     7621 tggcaggaac tcaggtaaac tataggtcaa ataatttaag ccaacttgaa agaaatatta
     7681 atgatattgc caataaacaa gttagaagta aaggaaatca gacagtaaca gacaatggga
     7741 ctccaattat aggacaaggc gatgatgctg ataaagattt gtttatgaag attcttgtag
     7801 cacagatggg taatcaagac ccaatgaatc cacaagaccc aacagaatat gtatcacaat
     7861 tagcacagtt tgcaacttta gaaaagatgt cagctatgaa tgataagcta gatatacttt
     7921 tagccttaag caattccact ctaataaatt ctgctttgag tatggcaaca tcattaattg
     7981 gtaaagaagt agaagtatct ggggaagagg aagaagatga aaacattgta ggagtagtaa
     8041 aatccacata tataaaagat ggggaagtac acttagaatt agaagtagat ggaagtgaca
     8101 aaactgtagg tgttaaatat gaaaaattac ttaaaatagg agaggtagat gcaaattcag
     8161 gagaagaaaa tgactcttca gaacaagaat ctccagaaga agtataatat taaatgagaa
     8221 aagaggtaaa tttatgataa aagcaatgta ttcaggtgta agtggaatga aagcaaatca
     8281 aacaaaacta gatgtaatag gtaacaatgt agccaacgct ggaactactt cttttaagaa
     8341 aagttcagct agaataacag attcatttta tcaaactgta ctatatgcaa gtgctccaac
     8401 agcggcatta gggggaacaa acttaggaca agtaggtgta ggtagtaaga tatcaagtat
     8461 aaataaagat atgacacaag gaaatgtaca accaactaat aggccatctg atttaatgat
     8521 agatggagat ggatatcttc cagtagtaag acaaggaaca gttatgtata cgagagatgg
     8581 ttcttttaat ttagatacag gatttaaagc agctgatggt gctttgaaga atattaaagg
     8641 tggaagactt gtaaatggtg atggatatat gttacaaggt gtgatttatg atggggaata
     8701 taaagctgat actggagaat tcattaaaaa gggtgatgct aaaggaaaag agcctattgt
     8761 aataccactt caaagtataa gtcctaagga tggaacagtt caaaatgtac tagagtataa
     8821 tatatcaaag gatggaacag tagaattttt attgagtgat ggtcaaagaa ctcaatttgt
     8881 ctatgatata cctgatggtg ctaaggtagg agatacacct gctggaatag ataaattaaa
     8941 aggtacaata caaaaagtgc aaacatatgc attccaaaat ccagcaggac tagatgcaga
     9001 aggaggaaac ttattcaagc catcagcaaa ctcaggagat ccacaaatag caggagctac
     9061 aggaaacata atacaagggg caatagaagc ttccaatgta gatatagcag aagaatttac
     9121 agaaatgata atagcaagta ggtctttcca agcaaacagt aaaataatat cgacatcaga
     9181 cgaaatactt caagaaataa taaatcttaa gagatagtag gtacatatta tgataagagt
     9241 aacggattta aacggaagaa gatacatatt aaattcagac cttatagtga gaattgactc
     9301 tgtaccagaa agtaggattc tccttacaac aggagaaaaa aatcttgtaa aagagtcagt
     9361 agatgagata gtagataaag taataaagta taaaagaaaa gtaaatgtag gttatattag
     9421 gagtgaaaaa taatgaaaaa aacagattca ctaacattca ttggactaat attatctaca
     9481 gtattagttt tatttggggc agtaaaagga agtagttcag gattgaaaag ttttttcgat
     9541 tttgcatcta tattaatcac agtatttggg tcatttggag ctttgatgat tacatttact
     9601 attgatgata taaaacttat aaaagatgcg ctacaatact cattcaagac aatgaatata
     9661 tcgaaattag acttattaaa tcaatttaaa acactttcta aaaaagcaag aagagaagga
     9721 cttttatcta tagaaagtga tgtaatgaaa atacaagacc catttatgaa aaaagggtta
     9781 gagttatgta ttgatggcct tgaagtaaat gaaataaggt ctatactaga actcgaaata
     9841 gattctgttg aagaaaccaa taacagatat tctaaactat ttaaaacttg ggggactttc
     9901 gcacctgcat ttggtatgtt gggaactcta ataggtttaa tacaaatgtt atctgacctt
     9961 acatctccag atttaatagc atcgggtatg ggaaaagcat taattacgac attttatggt
    10021 tctttacttg ctaatatcgc tttgaaccca attgcatata atatagatga gaagactgaa
    10081 aaagagattt atgtaaagga aatgatgctg gaaggtataa taagcataca atctggggag
    10141 agcagcatag tagttgagga acgtttagct acataccttt caaacaatga gaagctagaa
    10201 attatgaaat ccaataaaaa tacagaaaga gcaatgagta atggcgcgta aaaaaaataa
    10261 gaaaaaggat gagataaatc cagatgcatg gttggctact tatgcagata ctataacact
    10321 gattcttaca ttttttgtgt tattgtatgc tacatcagtt gtagatatgg aaaaatttaa
    10381 aaacatagca gatgcgttaa aaggtcagtt ttctgggata agtatgttca gtacaggttc
    10441 aagtacaagt gagaagtctc caatagagag tgttttagtg gataaaaatg ataagtcaaa
    10501 tttagaacag gatttaaaag ataaagttaa taattctaac ttacaaaata gtgtaacagt
    10561 caaagatgat agtagaggga ttttactaga actagatgat tcaattttat ttgactctgg
    10621 gatttctgaa ttaaaagcag atagtaaagc cactcttaat aaagtatatg acatgataaa
    10681 gatgatgaaa aataagatag ttatagaagg ccatactgat aatgtacctg taaaatcaag
    10741 tacacatgaa tccaattggg atttatcttc tagtagagca gtaagtgttg taagatactt
    10801 tgtagaagaa aagggcatgg accctagact attttcggca acaggatgtg gagagtatca
    10861 acctttggtt tcaaatgaaa cagcaaaggg aagggctgag aatagaagag ttaatatact
    10921 tataactatt gactaggagg aagaggtttg ttcaaaaata aaaaaattat tataattatt
    10981 ttgatactga ttattttctc agcaggagca ggttttttta tattaggaga aggtggaaat
    11041 aaacaaattg acaagattgc aggtttgttt attccaacag ggaaaggcaa agatgaagta
    11101 aagaccgaaa atcttgactt agaagagatg gtattaaagc tagctgatga aggtgtaaga
    11161 tatttaaaag tatcaataac tgtatcttat gattcttcat ataaagatat agaaaaaaat
    11221 acgcctttaa ttagggataa tattataaat tgttttatgg caaaaagggc agatgatttt
    11281 actgctgata atttaaagaa cataaaagaa gaaattaaaa atacattaaa tatacaacta
    11341 ggtgaaaatg taattcaaaa tatatatttc acaaatatag tagtgcaata aaaggatggt
    11401 aagacatgat actttattta ctaaaaatat taatatgtat cccaataatt ttaatattga
    11461 tagtcttatc tttaaaaatt agccaaggaa ctttattaaa aaaatataat gataaatata
    11521 taaaaatact agatattgta aatataagta aaaacaatag cataataatt ttaaaaatag
    11581 gtgaagaagg atgcattatg gcatcttctt cctctagcat gaataaaata aaagatttga
    11641 ccaaagaaga aatattaaga atagaaaacg acattaaaga aagaaatttt aaaattcaag
    11701 attttaaaat ggaaagaatt aaaaagaaat atgatttcag tagatatatt ggaaaattta
    11761 caaaaaagga agataagtaa atggaactat tatcaaaatt acctttggct gatgtaccat
    11821 atacaagttc aatgcaattg tttttatttc taactgcatt aatgttttta ccatttgtaa
    11881 tgtttatgat gacaagcttt gtcagaatag ttataagttt atcatttttg aaatcagcat
    11941 taggagcaca gcaagcaatt ccaagccaaa ttttggtagg gcttgcaata gtattgacaa
    12001 tatttataat gagaccagta ttaaatgaga taaacgaaaa ggcattacaa ccatatatga
    12061 aagaagaagt tactatggaa gaagctatga aggaagcaga gggacctatt aaagaatttt
    12121 tattaaagca aaccagacag acagatttag atttatttgt agaacaggct ggtttgaaag
    12181 aaaagaaatt aactcgtgaa aatataccac tatctgttgt agttccagca tttgctataa
    12241 gtgaactaaa aactgctttt caaataggtt ttttaatata tataccattt ttgataatag
    12301 accttgttgt ggcaagtgtt ttgatgtcta tgggtatgtt tatgttgcct ccagtcatga
    12361 tatcattacc gtttaagtta ttattatttg taatggtgga tggatggaac ttgatagtaa
    12421 aaacattgat attgggattt ggataggaga aaaatagtat gagtgaattt atgataatga
    12481 gtgttcttaa aaatggcatg tttacaggag ctatgattgc agctccagta ctcattgttt
    12541 ctcttgtagt tgggctttta ataagtgtgt ttcaagctac aactcaaatc caagagcaaa
    12601 ctttgacttt tgtaccaaaa ctaatagcaa ttcttttagt agtagctatt ggtggaagct
    12661 ggatgttaca tactcttatt agctttactt cggagatatt caatatgatg tctcttataa
    12721 ctaaataagt aggatatata tgatttttgt atttattaga atagtggcat tctttggaac
    12781 actaaggata ctatttccag ctggtacacc tgctttattt aagtcgctct ttgcaatcat
    12841 tatatcaata ttgatttcaa gtactatgca aattgaatat gcaacaaata tagacaacat
    12901 tgtattgttt accttatatg gtataaatga gaccataact ggcattatgc ttggatatat
    12961 aaccaatcta tgtttttata gtatcagaat ggcaggttct atgatggatc aacagatggg
    13021 attatcaatg attaatatgt ttgacccaaa ttcaatgact cagacaactt taattgataa
    13081 tcttatgaac tggacagcac gtatgatatt ttttagcatg gatggtcatc atgttttaat
    13141 tagaggcata aggtatagtt ttgagttaat tccaataggg aaatcattcg tagataataa
    13201 tatagattat ataataaaca tatttgtgca gtgcttttta actggattca aaatagcaat
    13261 acctattgta ctttgtctgc ttatggcaga ttttatatta gggcttatat caagaagtgt
    13321 acctcaacta aatgtaatga taattggtat gccattaaaa atactggttg gtattgcgtt
    13381 atttatcata tcaatacctt taattgtcaa tcaaataagt catttattaa gtcaaatacc
    13441 aaagatgtat gagggaactt tcgcactagc tcctatgttt tttatgggtt caacagataa
    13501 aacagaagag gctactccaa agaaaaaggg agaacaaaga aaaaaaggta atatagcaaa
    13561 gagtaaagaa ttacccgttg caatgactct acttgcattc actttattag tacctacatt
    13621 attttcatat gtagtggata cacttaaatc ttctcttaat tattttttga gtctagattt
    13681 ttatatgaat ataaattatt caagtataga gaaattagtt atagcaggat taatggattt
    13741 ttttaagata tttttaccaa tagctattcc attcttagtt ttaggtatag tcgcaaacct
    13801 atttcaagtg ggaatactgt ttactggtga aactttaaaa cccaatttat caaaattaaa
    13861 tccagttagt ggatttaaaa acatgttttc tatgagaagt ttgtcaacct taataaagga
    13921 tacagcgatt atatctattt tggcttacat aggatataca ttcttccaag ataattattt
    13981 agatatatta aagctaggta acatatacct gccaacttta atgtacacag ttaaagattt
    14041 agtatattca atattaagta agatttgtgt agctatgata gttatagcag ttgcagatta
    14101 tgtttatcaa agatacagtc ataaaaagca acttagaatg acaaaacaag aagttaaaga
    14161 tgaatataaa aactcagaag gagacccaga aataaaagct aagataaaac aaaaacaaag
    14221 acaaatttcg tctcaaagaa ccatgcaagc agtaccaagt gctactgtaa tagtaaccaa
    14281 tcccacccac ttatctatag cagtgagata tgaaaaaggg aaagaccaag ctccagtagt
    14341 ggtggcaaaa ggtgcagatt atttagcttt taaaataagg gaaattgcaa agggaaatga
    14401 catacctata atagaaaata aacctattgc taggttgtta tataagcaag tagaaataga
    14461 ccaagaaatt ccagaggaca tgtatcaagc ttttgcagag atattagtag cagtatataa
    14521 aatcaaaaat agatacaaag tcctcaaaag gtaggataag taatgaataa gtttaattta
    14581 aaggaaaata tggacgtaat agtggcattt ggtgttgtag ggattgttct tatgatgata
    14641 gtgccgttac ctacatttat attagatatt ttactggcaa ttaacataag tttatctgtg
    14701 ctaattttac taatgacatt atttacaaca aatgtacttg atttatctat atttccaaca
    14761 gtgttacttg tgacaacact atttagattg ggattaaatc tttcatcaac tagattgatt
    14821 ttaacacagg gatatgcagg tgaagttata gaggcatttg gaagttttgt tgtaggtgga
    14881 aattacgtag taggaataat tatattctta attatattaa ttgttcaatt tgttgttata
    14941 accaatggtt ctggaagagt atctgaagta acagctagat ttacgttaga tgccatgcca
    15001 ggtaagcaga tgagtataga tgcagattta aatgcaggag ttatagatga caaaactgct
    15061 agaaaaagaa gaaaagagct tcaacaagaa gcagattttt atggctctat ggatggagca
    15121 aataagtttg taaagggaga tgccatagca ggtcttatag taacagcaat aaacataata
    15181 ggtggaattg taataggagc tgtaatgctg aatatgactc ttatgggtgc agcacagaca
    15241 tatgtaagac ttactatagg ggatggtctt gtaagccaga tacctgctct attaatatct
    15301 acatcagcgg gtataatcgt aacaagagct tctactgatg aaaactttgg tggattagta
    15361 agtaaacagt taacggcaat accaaaagca gtaatgatga ctggttgtgt tctaatcgta
    15421 cttggtatga tgcctggatt acctaaacca gcattttttt cactaggtat aggtgctata
    15481 gcagcaggtt actttttagg aagagaaaag aaagatactt tagaagactt ggagtttgaa
    15541 aaacctgata tggtaaatcc agataaaaat gaagtagaag atgtaacaaa cttaataaat
    15601 gtagaatcaa tagaagtaga aattggatat ggtttaatat ctttagctga tgaatcagca
    15661 ggaggagatt tacttcagag aatagcatca ataagaagac aatgtgcaat agacatgggt
    15721 atagtcgtgc aacccataag aataagagat aatttacagt taaatccaaa ccaatacaat
    15781 ataaaaataa agggaaatgt agttggtggg tatgagataa tgcctaatat ggttttatgt
    15841 atggacccta tgaatcaagg atttagtata tttggaataa agacaattga gcctactttt
    15901 ggaatagagg cactttggat agaaagtgac aaggtagaag atgcagagat aaagggttat
    15961 acagttgtag attcatccac aatacttata actcacttac tagatataat aaaatcaaat
    16021 gcacacgaat tactgggaag acaagaagtt aagactatta tagatgcagc aagagaaaat
    16081 tatagtgctg tagtagatga attgatacca gatttaatga cacttggtga aattcaaaaa
    16141 gtacttaaaa atctgttaag agaaaaagta aatataaagg atagggttac aatacttgaa
    16201 acgcttgcag accagtctag aaatacaaaa gatatagagc ttcttactga atatgtaaga
    16261 atagccttgg ctagaagtat ttgtaataat ttagttgatg atgataagac aattgtagtg
    16321 gctacattgt ctccagaaac tgaagagttg gtatctaata atttacaaag gtctgtaaat
    16381 ggaacttatc cagcagttga cccagaaaat acaaataaaa tatttgagag tatacaagaa
    16441 gtaatgaaca atgtatattt caatcataat ataccagtaa tagtagtatc accaaagata
    16501 agagctccat ttagaaaatt agttgaaatt gtatatccta atttgactgt attatcatta
    16561 aatgaaatac ctaacgatat taaaatcaaa gcagaaggtg tggtgagcat atatgatgac
    16621 gaaaaagtat acagctaaca caattcagga tgctatgaat ctagccaaaa tggaattggg
    16681 tgacaatatt acattaatag ataaaaaaga agtaagaaag tctggaatac aaggtatctt
    16741 ttcaaagaaa gatattgaac taactattgg atgggaaaag agagaaaacg tagaacaaaa
    16801 agatttaaaa cgagagatag aacaattaaa gttaattatt aacaatatgg ggtttgataa
    16861 taagaatgat aatgatatag ataaaatatg taaaaatcta ctaagtttag aattaaatga
    16921 agaaattgta gaatctataa gaattgattt gcaagaaatg aagttcaatg gaattgatac
    16981 aagcaaaaat ttagtagaaa ttttaaagaa aaagattaaa atagaaaatc aagcaataaa
    17041 tggtaaaatt gctctagtcg gaccaccagg agtaggtaaa acgactacaa tagcaaagtt
    17101 agccgcaaaa ttagtttttg aagaaaataa aaaagtagga gtaataacta tagatacata
    17161 caggattggt gctgtagaac agttgaagat atatactgat ataatgaata ttccttttaa
    17221 aggggtaata agtcccgatg aaatggaatt ggcacttgat gagatgaaag attgtgatgt
    17281 tgtattaata gatacaacag gtagaggcta taaaaactct atgcaaatac tagaaatcaa
    17341 aaatcttata gataaagctg aaactgataa tatacattta gttgtgaatt gtacaacaag
    17401 agaaagtgat acaaaagcaa ttatagattc atatagaaat gtgaatttta aaagcttaat
    17461 aattacaaag ctagatgaaa caataacata tggctcaata tttaacataa tgaattatgc
    17521 acaaaaacca atatcatata ttacaacagg acaaaatgtt cctgatgata ttataaaacc
    17581 taatgaagat aagattataa ggttactatt aggagttgaa agtatatgat agaccaagct
    17641 caaattttga gaaaaatggc aatggaaaaa agaggacttg atgagtttat agtagagaat
    17701 gagaatgtac ctaagataat tacaatagct tctggtaaag gcggtgttgg aaagagtaat
    17761 ctcgctacaa atttgtctat atgcttgacc aaacttaata aaaaagttct tatattagat
    17821 gcagacatag gaatgtctaa tatagatata ataatggggg taaatgtaaa agggactata
    17881 attgatgtaa ttaatggcga aaaaaatata gaagatataa tttcacagac taagtatggt
    17941 gtaaatataa tatctggagg ttctgccctt aatcatatag aagactttac agaagctcaa
    18001 agaaataagt ttatacatag catagaacag attcacgatg tggattttat aataattgat
    18061 acaggagctg gtatgagcaa aagcctattg tcatttatat actgtagtac tgaatttttc
    18121 ttaataacaa ctccagaacc gacatcattg actgacgcat atagtttatt aaaagctata
    18181 agtaattttg gcataaagag aagtgcaaat ataataatca atagggtaat tgatttagaa
    18241 gaagcaaaat ctacatacaa aagaataaaa atggttgtgg acaaatttct aaaccttaat
    18301 ctagaattgt atggatatat aatagatgac aaaaaagtaa gtttatgtgt aaaaaaacag
    18361 ataccattca ttttagaata tcctacaagt attgcatcaa aatgtattat agccatagca
    18421 aagagaatgg taagtcagtc aagagaagat attgttgata ataaaagttt tctaagaaag
    18481 atgtttagta tatttaaaag ctaaggaggc gtagttaatg aatagagaag aattaataaa
    18541 agagaatatg cctcttgtaa agagtatagc aagaaaattt tttataccag ggaaaggttt
    18601 tgaatatcaa gacttagtaa atagtggagt cattggattg attgatgcaa taaataaatt
    18661 tgatgctgaa aagggggcaa agttttctag ttactcttat ataaaaataa aatctgctat
    18721 attagatgaa attagaaatc aaagtccaat ttctaaacat aatctaacta aagtaaataa
    18781 gtataataga gtggtagaaa agttgcaatc taaattatta agagagccaa cttcatatga
    18841 aattgcaaaa gaattaaatg tttcagaaaa agaacttcat gatatcgaaa gtaatataga
    18901 catgttaaat attgtatcat taaactatgt aatctttgaa gatacaaatg aaactgtaca
    18961 agatgtaata agtgatagag aagaggaagc tccagaaaat ataatagaag aagaagaaaa
    19021 gttagagatt ctatctaaag caattagtaa tttaaatgaa agagaaaaat taatactttc
    19081 actgtactat tatgaagatt taaatctaaa ggagataggg aaagtgctag gtgtttcaga
    19141 atcaagagtt tcacaactcc acagaaaatc aataagaaac ttaagaaaca agataaaaga
    19201 acttaaatat tctatataga tggtgattga taagatgata caaattatat taatagtagt
    19261 gtctatttta ataatagtaa ctatagttat aaatttacat aaggaatcaa aaaataaaga
    19321 taacagatac tttgataaaa tatttgagca agaatatgaa gcaaatgata gattaataat
    19381 tgcagaaaaa agaagattct ttttagacaa agtacttgct gaaataagaa gtaataatca
    19441 tataaaagtt tatgaaaatt ataacctaat aaaggctaat gaagaaagaa aggaaaacat
    19501 atttatagaa tcaaaaaagg aagtaagaca agaaaatgag ttatctctta aagtgaaaca
    19561 tcttataagt tcaggttatg atgatgtgga aatatgtaag atgctagata ttggaaaggg
    19621 ggaactgtca ttaataagga gttgttacaa aatataagga tattattatg tgataaaaaa
    19681 gtattcttag gaataggtat tggtatatta atatcaactt tatgcataat gctttttagt
    19741 aataatgata tgagtaaggc agagatagaa aaaaaggcca gagcgttagg catgaaatat
    19801 acaagtgaga tgaaagtttt agaatagatg atactgttta gacatttaca aaaaaggagg
    19861 tcataatgtg ataagaggat tatatacagc agtttctgct atgataacta atcaacaaaa
    19921 gcaaaatgtt gttgtcaata acctagctaa tatggataca aatggatata aaagtaaaca
    19981 acttttgact aaaagttttg atgaattaaa gttggagggt tacaataact acgcaaatgg
    20041 aactaaacaa aagcagatta taggcgatat aagtccaggt atttctatgg atgaaactat
    20101 cacaaatttt aatcaaggac ctataaaaac tactgataat aaaatggatg tagctattca
    20161 aggtaaggga ttttttctag ttagtgatgc agctggaaat caattttaca ctagaaatgg
    20221 gaacttcaga acagacaatc aaggtgacct tataacaagt gaagggtatt atgtacttgg
    20281 gacgaatact gctactggtg ctgtaggtca tataaatgta ggaaatcaaa aatttgaagt
    20341 ggcaaaagat aatacaatat cactagatgg aaatgctatg tataaattca atattgtaga
    20401 ttttaatgac tacaataact taaaaaaaga aggagataac ttgtattctg gaggaggagc
    20461 tatgaaagta aataatgcac ttacaataca aagtgcgata gagtcttcta atgtagatat
    20521 ggtatctgaa attaataata tgatgaccat atcaagagaa tatgaggcaa atcaaaagat
    20581 aattcaggct atggattcta aattagcaaa aatagcaagt gagattggtt cagtaagata
    20641 ggtgatagga ggtgaatttt tatgtacaca atgttttata ctagtaaaac agctttatca
    20701 gcaaatcaaa gtaaactaga tataatatct aacaatatag caaattctga ttcaacagga
    20761 tataaaaaaa taaatatgga atattctgat ttagtggatg aagtactaaa tagaccatca
    20821 tatcctacaa atggcaatga tatatcgact ggaacaggag ctaaggcaac agctcccatg
    20881 agaatttatg aacaaggtgc attggcccct acagatagta agagtaattt ggcgatagat
    20941 ggagaaggat tctttagagt gataagaaaa gatggtacat atgcatatac aagaaatggt
    21001 ggatttaata tagatggttt aggtaagatt gtagatgata atggaaatat attagatgtt
    21061 caatttgatg caggatataa ctataataat acgaatatag atagcacaaa cttaacagtt
    21121 tcaagaaatg gcgagttatt cgtagataat aaaaaaatag gaaaaatcaa tttatatcaa
    21181 cctataggaa ctcaaaattt tatatctgaa ggagatagtt tattcgtcgc aactgatgca
    21241 gcacagatta aacaagttga gaaagtagat atagttcaag gctatagaga gaggtcaaat
    21301 gtttctttac aagaagaatt tgttgactta atagccactc aaagagcatt tcaaatgaac
    21361 tcaaagggaa taaagacagc agatgaaatg tggcaaatag ctaataattt aagagctaaa
    21421 taattatgta aggtgaggaa tattcatgaa tgatatgaaa gagtacgatt ttggcaaacc
    21481 tcagagttat tcaagagaaa atttaacttg tttaaatttt ttattagctg aattttgtaa
    21541 aaaatataaa aactatataa tttatgaact taagtgtaat tctagtatga ttgttgatag
    21601 tatagaccaa gtaaattatc agactttttt agatagagta acttcttcat gtgtagtagt
    21661 acaaaatgca atggaacctg ttatgaaaaa cttttctttt agaatagata gaagtgtagc
    21721 tgatatgtgg attgatataa cttctggagg aactggagtt ataaaggata atgatagaga
    21781 gcttacagaa cttgataaaa aagtgctttt acatcttatg gatgatttag taagaaatat
    21841 gcacttattt gaaggttttg aaaatataca agtattggat acacatacac atattaattt
    21901 accacaatta tgttctccaa ctgcaccagt atgcgttgta gatttaaaag tgctaaatga
    21961 taatgagtat attggaagtg tttctctttg ttttccgtat aatggtttag aggctctatt
    22021 agaaagtata tctgttatag aattttttga taatgatgta ggagatgatt cagaggaatt
    22081 taccaaaaaa atctataata gtgtatctaa tatagaatta tcagttattg cagagatagg
    22141 aaaagtatca attaatgttg aagacttact taaactagaa gttggagatg taatagtaac
    22201 aaataaaaaa ataaatgatt atatagatat atttgtagaa aattcaaaat catatacagc
    22261 aacaccagga tttatatcaa gcaaaaaagg tgtaaaaata aatgatgctg ttggaaaaga
    22321 ggtgtagtta tggacaatag taatcttatt tcagatgaag aagcaaagat gttattaaat
    22381 gataaagttg aaagtgaagt tgatggggat gatgagttag ccttagatgt ctctgaagtt
    22441 aatcgaaata atatacaaag gatacttgac cttaagttag aattatctgt agttataggg
    22501 agaactaaga tgactctaag agatatttta aatttacata aaggttctct tattgaactg
    22561 aatactttag cagaccaaga tgtggaaata ctaatagata ataaggtttt ggcttatgga
    22621 aaagtagtgg tggtagattt aaattttggt gtaaagataa caagtatagt aagtgaagaa
    22681 gacatggtaa aatctcttgt ttagagataa catcaattaa tacttaaggc atgaggtggt
    22741 acttatttta aacttaaatg atataaaaaa agatatacta aaactcaata aaaaagactc
    22801 atgttctcta tataatgaat ctattaataa tataaataat aattgtatat caactggatt
    22861 aaaattttta aaacaagcaa gagatttaaa cccagatgat gttgatatat taaatctcac
    22921 aggtcttgta aacttactaa aatgtaattt tgatgatgct gtagaaagtt tctataagag
    22981 ttcactttgt gaaaaaactg ctctgtgtga aaagtatata aacatgctta catctgagga
    23041 gtttttaatt ttctttgaaa agtacaatca acttataagg ttaataaata aaggaatgta
    23101 taaagaagct atacaagggt ttaaacttat aactgaccaa tactgtgatt taatagaacc
    23161 ttatgagttg cttgccttac tgtatgacaa ggaagaagaa tatataaaat ttgatgaatg
    23221 tttagaagta ttgaagatta ttgataagga aaattattta ttaaatcaag aaaataacac
    23281 attagatatg tataatagtg ctaatataca aactaaaagt aatgtaaaag tagatgatta
    23341 taagaatgag tttttacata aaagtagtag taaccataaa attaatgatt ataataatgt
    23401 aactaaagaa aaaagttcaa gcaaaaacca gaaaagttca gattctaata agaaaaaaag
    23461 tattaaaaaa aataagtacc tatatatagc cataatatta ggaatattat tagtaggaca
    23521 aagtatatat agttcttata agatgaataa tttaactgat aaaatatcta aaaataatga
    23581 aactaaaaat gaagagtcaa tagattctaa cttgactaag aaagaaaata atatagaaga
    23641 aaatgaagat atgagtaagg cttctaatat aaataagagt aataaaaaca ttaataaaga
    23701 ggctgatgta aaatctaaaa aaaatactgt agaaaatgaa gatgataaag attctgactt
    23761 atttacagag gatgaattga tgtctaaggc gaatacttta aaatcagaaa aaaaaactaa
    23821 atcttctata caactatata agaaagttgc agatataggt gaaaataaag ggaatacatc
    23881 tgaagcaact tatcaagttg ctattctcag tgaaaagtta aaagactata aaactgctga
    23941 agaatattat aaaatgtatg tagaaaatta ctcagaaaaa gatgcttatt ttgatgaatc
    24001 ttattataat ttagggatga tgtactataa caatggtgat ttaaaaaatt ctaaattgac
    24061 attaaagaag ttagttaata aggtaccaaa tagtatgtat aataattcaa aagtaaaaga
    24121 aattttaaaa gaagaatagt taaattagat aagaaaaata aattatttta ttttaaaata
    24181 atatgattat aatatattga cataccattt taaaaaattt aaaatattaa ttataaaatg
    24241 aggagggata aaaaatggaa tttgaaaaag ggagtatttc aattcataca gaaaatattt
    24301 ttcctataat taaaaaatgg ttatactctg ataaagacat atttataaga gagcttataa
    24361 gtaatggatg tgatgcagta agcaaacaca aaagacttgt atcactagga gaaatatcag
    24421 aaaataagtc atcagattat aaaattacag tatctgtaaa taaaggagaa ggtacactta
    24481 aatttattga caatggaatt ggtatgactg aagaagagat aaaaaaatac ataaatcaag
    24541 ttgctttttc aggtgctgaa gatttcttta ataaatataa ggataagatg gaagagtcaa
    24601 atgacataat aggtcatttt ggattaggat tttattctgc atttatggtg tctaaaaagg
    24661 tacaaataga tacactttca tatacagaag gagcgactcc tgtaagatgg atatctgaag
    24721 gtggtacaga atacgaaata tctgaatcag atgctagaaa tgatagagga actactataa
    24781 cactatttat tgatgatgat agtaaagaat ttttagatga atttactgta agaggaataa
    24841 taaataaata ctgttctttc ttaccagtag aaatatattt agaagatgtt gaaagattag
    24901 aaagagaagc taaagaagca gaagaaaaag ctaaaaaaca aaaggaagat ggaaaagaag
    24961 agattgtaga tgcaaaagta attgagcctt taaacgacac taatcctcta tggttaaaat
    25021 ctccaaaaga ctgtactgat gaggagtata aagagtttta tagaaaggta ttcaatgtat
    25081 ttgatgaacc tttattttgg atacatttaa atgtagatta tcctttcaat ttaaagggta
    25141 tactttactt ccctaaatta aaaaatgagt ttgaactgac tgaaggaaaa gtaaaattat
    25201 acaacaatca agtgtttgtg gctgacaata taaaagaagt tataccagaa tttttacttt
    25261 tattaaaagg tgttatagac tgcccagact taccattaaa tgtatcaaga agtttcttac
    25321 aaaatgacag ggatgtaagc aaaatttcta aacatataat taaaaaagta gctgataagt
    25381 taaaatctct ttgtaaaaat gaaagagaag aatacaataa gttttgggat gatatacaaa
    25441 tatttattaa atatggttgc ttaaaagatg aaagtttcta tgagaaggta aaagaatgta
    25501 tattattcaa aactattgat gatgagtata taacaataca agattattta gaaaaatgta
    25561 aggacaaaca tgaaaataag gtattttatg taagtgataa ggaacagcaa tctcaataca
    25621 ttaaactatt taaagaatac gacttaagtg ctgttgtttt aaactcatca atagatactc
    25681 attttatttc atttatggaa tataaagaga atggagttaa gtttaataga atagatgcag
    25741 acctttctga tgtattaaaa gataaaaatg aaaataaaga tagtgaagaa aacaaagaag
    25801 agatagctaa gatagaaggt ttgtttaaag aagctgttgg agaaagagtg aagaattatt
    25861 ctgtagaagg attgaaaaat gaagacactc cagctatggt attagtatca gaacaatcta
    25921 ttagaatggc agaaatgcag tcaagatttg ctggaatgga tttaggaatg aattttgagg
    25981 aagaaaaaac tcttgtaata aatgagaaca gtccaataat caaaaaattg gtttctctta
    26041 aagatgatga ggaaaagaaa gataagatta cgcttatttg taaccagata gcagatttag
    26101 ctttactttc taataaagaa ttaaagccag atgagttaga ttcatttgta caaagaagta
    26161 ataagcttat gagtcttttg atagaattat aagattgaat ttgaatacat tgaagcaaat
    26221 ttataaaaag tattcaatat cagtgatgag aaagaagtgt ctcaaaatga acttttcagt
    26281 tcatgaggcg ctttttttat gtgaaattaa atttatttta atcatttcaa caatgaaaaa
    26341 tatatttatc tctattttga gacagaccgt tctttatttt tttatctttg gttaattttt
    26401 aaatatttaa gtaggttcta ataaaaaact ctatttagtt aaatatagtt aacttgatat
    26461 ataataagat aatacatata attaagtaaa agattgtttt ttagtttatg tttaaataca
    26521 taattacact ttgttaaaaa cataaaatta ttactaaagt agaaatagga ggttttagca
    26581 tgaattttaa ttataatttg ccagtaaata tcttatttgg gagaggcaga attaatgatg
    26641 tgggaaaaga agtatctaaa tatggcaaaa aagtattgat agtaacaggt aaaaatagta
    26701 caaagaaaac tggattactt gataaaacaa tagatttact aaaagactct aaaatagaat
    26761 atgaagtttt tgatagagta gaacaaaatc ctcttataac tactatatat tcaggcgtag
    26821 aaattataaa atcaactgga tgtgattgtg ttttagggct tggtggtgga agcattatgg
    26881 atgctgccaa gagtatagct ttttcttata aaaacccagg agatttaaat gattatatat
    26941 ttggaattag acaaggtgaa gaagctttac ctataatctt agttccaaca acatcaggta
    27001 caggtagtga aggaaattgt tttgcagtac ttacaaattc agaaactaaa gataaaaaat
    27061 ctataaagaa gaatagtatg tatgcaaaag catcaataat tgacccagaa cttatggtta
    27121 caatgccaaa gcatataatt gcatcagttg gatttgatgc acttgcccat aatatggaag
    27181 catatctttc taatggaaga aaccctcttg cagatgtaca agctatttat gggattgaac
    27241 ttatatcaga aaacctaata aaggtatata atgatgttaa tgatttagaa gcatgggaaa
    27301 aaataacact tgcaagtact attggaggaa tgacgattgg tacagcgggt acagccttgc
    27361 cacatggaat ggagcatcct gtaagtggtc ttagagatgt aatgcatgga aaaggacttg
    27421 cagcgttgac tcctactatt atagaaagtt catgggatat tgatattgaa agatttggta
    27481 atatttcaaa ggtattagga ggaacttgtg cagaagattg ttcagatgca attagaaatt
    27541 tccttaaaaa aatagattta gatataaaac taagtgattt aggtattgaa aaatcagata
    27601 tagattggtt aactgataat tgtatgaaaa tatcagttgc aaatattaag agacatccaa
    27661 agtattttaa taaggaacaa ataaaggaaa tgtaccataa gtctttataa tttgacttta
    27721 catatttagt tttatataaa aattataaat taataataaa aaattataag taattaatag
    27781 taaaaaaatt gtgtatagaa gttatatata taataaactt gtcttaagct tttattttac
    27841 tttctaataa ataaaggagt ttaaaaaagg gaagattaat taagtaatat tttataaaat
    27901 tcttcgattt aaaaacttta agtagaggtg aaataaatgt ttaatcccaa aagaataata
    27961 ttcgaaaaag gaactttaga cactgatgtg ggcaaaaaaa tatacaataa atttagagat
    28021 aataaagatg tagagataat taatatatca tcaaatagga ttaaagaaca cattccaggt
    28081 gaaaatctat atgaacaata tagaagtggg aagcagacta tggtagttgg gattagaaaa
    28141 agtctgaagt ttcaaacatg taaaccttct gctaattatc aactaccact tgtcagtgga
    28201 tgtatgggaa gatgtgagta ttgttatttg aacacacaat taggtgataa accatttata
    28261 agggtgtttg taaatgtaga tgaaatactt gaacagacaa aaaaatatat tgaagataga
    28321 aagccacagg taaccatatt tgaaggggca gctacatcag acccagttcc agtagagcca
    28381 tatactaatt ctttgaagaa aacaatagag ttttttggaa gagaggaaaa aggaagattt
    28441 agatttgtta ctaaatacag tgatgtggat agcttattgg atgctgaaca taataatcat
    28501 acagaaatac gatttagtat caatactcct agaattataa atgaacatga gcattataca
    28561 gcctctgcaa ataaaagaat agagtcagct gtaaaacttg ctagggctgg ttatcagatt
    28621 ggtttcataa tagcacctgt gtttttatat gaaggttgga aagaagagta taaaaatctg
    28681 attataagta taaaagaagc tttaccaaag gattttgata aaaaaattat atttgaagtt
    28741 atatctcata gatatacaac taaagcaaaa aatagaatac tagagattta tccagataca
    28801 ctattgccaa tggaaaatga ggatagaaag tttaaatacg gtcaatttgg atatggtaag
    28861 tacgtgtatg ataaagaaca attagatgat atgaaagagt tctttcttaa ggaaataaat
    28921 ggagtattta aaaatagttt aataaaatat ataatatagc aaatcttacc taaagatttg
    28981 ctatatttta tttttatata ttttatgttt ataataaagc aatgagattt agcactatca
    29041 tataagaaaa ttaaaaaggg cactatctat caagaacatt ttttctagtt ttgatactat
    29101 aaagggtaag agtaacaaaa atatcaggag gagttatagt atgcatgcat gggagtctat
    29161 tcaaattaca ttggatttaa tagaagcaaa tttatcagaa gaaatatcaa ttaatgaatt
    29221 agcaaataaa gcaaatttat caccatttta ctatcaaaga ctatttaaaa gattggtaaa
    29281 taagactgta atggagtata taaagcttcg tcgtcttgca agagcttctg aatacttagc
    29341 agaacataag aatcgtatac ttgatgtagc tttaaatttt ggatttgcta gtcatgagac
    29401 atttacacgt tcctttaaaa aagcatatgg attaactcca gaaaaatata gagcaaatcc
    29461 agtaaaactc aatcacttta ttaagccaga attaatttta aattatgcta tggttgatga
    29521 aggtgttcca ctgattgcag atgatatagt gattgaagtc aatcgtaaga cattgagtaa
    29581 tccaaggaca tttgttggaa tagaaataga agtacctatt tgccagttgg tgggtggtga
    29641 aacaactgga attgcaatag tagaggaatt atggatgaaa cttggaagtc aaagacataa
    29701 tataccaaat caaattccag gtggaaatga atttgctgct ctttatatgg gaaatgcaaa
    29761 agaaggaaat tgtatgtata tggcaggtgt agaggtagag tcaggtacat cagtagaagg
    29821 atataatact tttgagttgc cagctaaaga atatttagta tgtggatttg aggcagaaaa
    29881 ctttaatgag ttagttaatt cagctgtttt taaagcagat aaatttatgg aaagatggat
    29941 gaagaaacat aatttaacaa ctactgattt tgcaattgag atgtattacc ccactacacc
    30001 tgaagcagca tatctggaac attggattgt tccggttcct attgaacaat agaaaggatg
    30061 atgaatatga attggcaaga tgcgtttttt caagatacta aacctacatt tgagcaaatt
    30121 aatgaattta ttaataatcc attatgggac aagttaaata aaactttggt ttctacttat
    30181 aatgtagaac ctaagcttga atacagtaaa tgttcaatgc aaagaggttg gaatgtaaaa
    30241 tataaaaaaa gaggtaaatc actatgtact ttatatccac aagaaggtta ttttaaagct
    30301 ttggttattg taagtgaaag caatagagta gaggttgatt tatttattaa tacttgttgt
    30361 gattatatca aaaaaatata taatgaggta aactttttta atggaagtaa atggctaatg
    30421 attcaagtag atagtttatt ggtattaaac gatatgcttg aactaattaa atttagagct
    30481 taacatatag aacaggatga atatataagt catcctgttt ttatattcaa tcagataaat
    30541 taatatatag tctataatat gaataattta gtaaatttac tttatctttc tacattcttt
    30601 gcaactaagt ccacatgtgt ttttttcttg catattagta gcaccccaat tagcaaattg
    30661 ttgtaaaata ggcacaatac ttttaccttt acttgttaat gtatactcaa cttttggagg
    30721 aatttcatta tattgtttac gatgaacaat accagtagac tctaaatctt ttaatgattg
    30781 agctagcata gtatttgtaa tgccatccaa ttttcttttt atgctactat atctaattgg
    30841 attttcaact gctaacatac atataatagg taatttccac ttaccaccga caatgtctaa
    30901 tgcataacgg attggacagg ttaattcttc aacgttttta gttatatttt tttcagtatt
    30961 tatactcatt ttaattactc cttagttata gtagtatctt ttttatactt tatcagaaaa
    31021 aactgcatac ttgttttttc tgtattttat attaaaatta tatcaaagga taatccttaa
    31081 tacaagtatt taaaaggggt gtaagaatta tgaaagcatt agaatttttg aacaaggcag
    31141 gagtttatta tttagcaaca gttgatgaga acaatcaagc acatgtgcgt cctttaggat
    31201 ttgttatgga atacaatggt aaacttacat tttgcacatc taatcaaaag gatatgttta
    31261 agcaacttat ttttaatcca aaggttgaac tttgttgtat agaccaaaac cttaatactc
    31321 tacgtatttt aggtgaagca gtatttgtta catcaaaaga aacacaagct aaggcacttg
    31381 aaataatgcc aagtttaggt cagatgtact ctgtgggaga tggtaagttt gaaatattct
    31441 ctattgataa ggcaaaagtt agttgttgtt caatgagtgg taaaaagatt gatgtagagc
    31501 tttaattata attgaatcga catccatagt gaaaattaag aaaacgttat tactgaaata
    31561 ttgatagatt taatttaagt gcaataaaaa gatttttaaa taatgaatat gtaaaaattt
    31621 ttagttatta gatgtgtatt tcaataatga tatactattt
//
LOCUS       DAMJWP010000018        31577 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.019, whole genome shotgun sequence.
ACCESSION   DAMJWP010000018 DAMJWP010000000
VERSION     DAMJWP010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31577
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.019"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            255..1685
                     /locus_tag="PC700_000780"
     CDS             255..1685
                     /locus_tag="PC700_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HDF2580104.1"
                     /translation="MDIKNKIKSIFLPEKNDYLDDEEFEYDIENNEEDLKEDNKVHLN
                     DLSRVKYDYRELKDIENQTEEMCLLAVKQDGTIIEFVKDKTYKVCMEAVKQTYKALKY
                     ITNQNEDICIEAVKQNYRALYYINNKTENVLIEAIKNASTYDVMEVFKFVEDQTEDVC
                     LAFIERASKNDVAEILKGIKEQTPAICLEAVKKDGKSLAYVKEQSNSICLEAVKENYS
                     ALSCVKEQTEEICIEAVKQNDFALYYVNEQTEKICMEAVKRSYMALQYVNKQTEEICL
                     EAVRIDGRALQYVKEQTEEICLESVRQNGKVLQYVKKQTENICIEAVRGSFEELEIKE
                     ILSYVKIPTERIFVEAVKQNGKILKYVENQTELICLEAVRENYNALAYVKEQTEEICL
                     EAVNQSYEALKYVKEQTEEVCLKAVKQDYRMLKYVNNQTEKICLEAVKQNYRALEFVD
                     NQTEKVCLEAVKQNRKALQYVKQKQS"
     gene            2053..2631
                     /locus_tag="PC700_000781"
     CDS             2053..2631
                     /locus_tag="PC700_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDF2580105.1"
                     /translation="MFKRYAEKMTSVLICNNMIDNNESKVYSYGFEILIAFIVNITTM
                     LFIGFLFGKFTYVLFFLMCYCPIRQFSGGYHADNYFRCLLTFIFIILSTILIIENINI
                     DLFKNIIMIIASVSWVGICVLCPIEHRSNPISDREKFVYKKTAIFISTVVLLITILSL
                     SLSIFVDYFTYSAFAMFWIFVMLVLGKLKAKV"
     gene            2661..2807
                     /locus_tag="PC700_000782"
     CDS             2661..2807
                     /locus_tag="PC700_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HDF2580106.1"
                     /translation="MKKFIVRFMKFASSLALSTAILSANSTCPWIIHQPKVPKEISNL
                     KKTN"
     gene            3112..4491
                     /gene="scfB"
                     /locus_tag="PC700_000783"
     CDS             3112..4491
                     /gene="scfB"
                     /locus_tag="PC700_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HDF2580107.1"
                     /translation="MSMIHKFSMNGYNIVLDVNGGAVHVLDDVAYDLLDFYKEKSKEE
                     ILEILKSKYQEEKINEAYEEILNLEKEGLLYTEDTYQYHPSFVHREPVVKALCLNVAH
                     DCNLKCKYCFAAQGDFGGEKELMSFEVGKAAIDYLIANSGSRKNLEIDFFGGEPLMNF
                     EVVKQLVDYGRSVEKDYNKNIRFTITTNGVLLNDEIIDYINENMHNVVLSLDGRKEVN
                     DNMRPTLNDKGSYDITLPRFKKLVEKRAKDKYYYIRGTFTRDNLDFSKDVMHFADLGF
                     KLTSVEPVVGDESNPYALREEDLPKIFEEYEKFAVEYADRQLQGDGFKFFHFMIDLNQ
                     GPCVIKRITGCGAGNEYLSVTPNGDIYPCHQFVGNEEFKMANIFDEEIVLPENLKNMF
                     REAHVYTKEECKQCWNKFYCSGGCHANAINFNNDISKPYELGCEMQRKRTECSIMIQA
                     KLMLEGATN"
     gene            4520..5017
                     /locus_tag="PC700_000784"
     CDS             4520..5017
                     /locus_tag="PC700_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HDF2580108.1"
                     /translation="MIRDYLEDKPLIDESVFVAKSADVIGNVKIGKDSSIWYNAVVRG
                     DEGPITIGENTNIQDCSIVHGDTETIIGNNVTVGHRSIVHGCKISDNVLIGMGSIILD
                     NAEIGEYTLIGAGTLITSNKKFPPGVLIMGSPGKVVRELTEEDKKYIDESYEWYLEAA
                     QNQKY"
     gene            5315..6979
                     /locus_tag="PC700_000785"
     CDS             5315..6979
                     /locus_tag="PC700_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HDF2580109.1"
                     /translation="MRISYRNLKRYREIGYVLIKYGFSFIVERLNIEGIAYKIPLFDP
                     PEEIKNMTTGERMKRVLEELGPTYIKIGQILSTRKDLLDQDIIDEISKLRDDVEKFDS
                     NIAIDIFKEEVGLSIEEIFLEFKEEPIAAASIGQVYEGVLKTGEEVIVKIQRPNIEKI
                     IKSDLEILRTIANTLKDLKKDFNLDLVQMIEEFQTQLMRELDYTFEAINATKFSRIFK
                     NSDEVYIPKVYSEYNTKKILVMEKVNGTKLSDVEKIRRLGYNTKTIVEIGVRSFFTQV
                     LSHGFFHADPHPGNIFVVAKNKIAYIDFGMIGIIDNKTLNQLNEIALAGVEKNVDKII
                     YLLIEMDALNGEADIKGLRQDLLYLIHYYYDISIEKINVTDILNELFRFFRQYKIVMP
                     AQFVTLAKTVITLEGTSRTLNTDFSFGSMGKEFMKHHYKSKFNPKNVVLSSRQNVEEI
                     LLDIKTIPKQLKAILKNIERNNIKMQIEDVKMTRLENCIIELTSQISLSLVLASIIVG
                     SSLIIASPNIENNIWIKFTAIAGFFISFIIGLCLVIRSIRSKYKKD"
     gene            6992..9442
                     /gene="recJ"
                     /locus_tag="PC700_000786"
     CDS             6992..9442
                     /gene="recJ"
                     /locus_tag="PC700_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDF2580110.1"
                     /translation="MKKWILKYKGKIEESEFSKKLNISPEICQILKNRGICTEKDSEI
                     FMNPSLDYLRDPFLMKDMQKAVDRIKLAVEKNEKIWIYGDYDVDGVSSTSILCLYFKS
                     IGYNVDYYIPNRLEEGYGINEDAIKLISSRGCDLIISVDCGITSVSEVNTANDLGIDV
                     IITDHHECQSEIPSAFAVINPKQEDCNYPFDSLCGCGVAFKMIQALTPKEEFKTSMYN
                     YLEIVTLATICDIVPLIDENRIIVKNGLKSMKEGKNIGLRELIKVCGVESDKIGSSHI
                     GFAIGPRINASGRLGYSYLGVELFTTQSQEEAVEIASILEEKNNERQMIEAKMYHEAE
                     EMLKSNSRYNDDKVLVLAKEGWQHGIIGIVASKLTEKYYKPTILLGIENGEATGSARS
                     IKGFNIFEALIKCKDLMTKFGGHEQAAGLSLDSDNVEILANEINKFADYNLTEDDMIE
                     NVNVEFELQENVINLNLVEELHKLEPFGLNNPNPRFIVRNYILKDLKVIGKNQQHLKL
                     SIEKEKSYECIGFNMSHLKSMYKVGDKVDVLFQLDENNYMGNRKVQFLLKDIRLARPK
                     SASNDKLSLKLMSKIIPKDTQSLYNISVSDFELFDGNTDINIFDYFEKDTLIISNSIN
                     GFYRAMSDISLIDLDFNINYNIIEDVSKNTDKLELIFSPNIDKIDLKRYNNIILYDYL
                     YNKGEYSYIYENKREESEIIKYYNKTDLLYLKNVVSNIVPSRDEFITIYKQALIKKEI
                     DLDMVNIRETFNVIPLKFFTILNVFRELNLLDFNLNYEKNSVLIRILPKPQKKLDLNE
                     SLILNNLKNLEKQYNSSY"
     gene            9455..9967
                     /locus_tag="PC700_000787"
     CDS             9455..9967
                     /locus_tag="PC700_000787"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDF2580111.1"
                     /translation="MDLKNFIRNIDDFPKPGIDFKDVTTLFKDGDAFKYAVDSIVEEL
                     KDKDVDLVIGPEARGFLMGTPVAYALGVGFVPIRKPGKLPGEVESYEYGLEYGTDTLE
                     IHKDAIKKGQKVAIVDDLLATGGTMEAAAKLVEKLGGEVVSMQFLIELKFLNGREKLS
                     NYDVNSLIKY"
     gene            10041..12248
                     /locus_tag="PC700_000788"
     CDS             10041..12248
                     /locus_tag="PC700_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003229747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HDF2580112.1"
                     /translation="MHDKELQEIIEKIKVYAPNANFEMVEKAYNLAKKAHEGQYRKSG
                     EPYFIHPLAVANILIEMELDIETITAGLLHDVVEDTEYTYEDIKKEFGKEVADLVDGV
                     TKLGQIKYRSKEETQSENLRKMFLAMAKDIRVILIKLADRLHNMRTLKYMPPEKAKYK
                     AKETLEIYGGIAHRLGISKIKWELEDRALRFMDPEGYYDLVSRVSMKRSQREDYIQGI
                     VELLKDKFKDLSIPCDVYGRPKHFYSIYRKMQKKHKTFEEIFDLTAVRILVDNVKDCY
                     AVLGIVHTLWRPIPGRFKDYIAMPKPNMYQSLHTTVVGPDGEPLEIQIRTHEMHNIAE
                     NGIAAHWKYKEGISSKESKVEEKLQWLRQMMEWEKDLKDPQEFMDALKEDVFNSQVYV
                     FTPKGDVIELPAESTPIDFAYRVHTNVGNKCVGAKIDGRIVPIDYKLQNGNIVEVLTS
                     SNSNGPSRDWLNIVKTPNAKSRIRQFFKKERREENVDRGNTILEREFKKHDIPQKDPL
                     VEKYMALVAKKFNQPSVEDLIATVGYGGIMSSQVVSKVKDLYLKEVKKAKKEQQIQEE
                     DLNKHNISETEYSKKRKKNASQGIIVKGLDNILVRFAKCCNPLPGDEIVGYITKGRGV
                     AIHRADCPNANNNGDFFQNRLVEVSWSSAGKGKFEAEIQIKAIDRRGIVNEITHIVAM
                     DKLSLNGINARKGKDSIVSINLLVEVNDTEELKILMKKLKAIPGVESIYRMTN"
     gene            12273..12722
                     /locus_tag="PC700_000789"
     CDS             12273..12722
                     /locus_tag="PC700_000789"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDF2580113.1"
                     /translation="MRAVVQRVSSSKVTVDENTIGQINKGLLVLLGVTHDDKSSDVDY
                     MIDKILNLRIFEDENDKMNLSLMDIGGELLVVSQFTLYGDCRKGRRPGFSNASRPELA
                     NNLYEEFVKKAKDKGVTVGTGQFAAHMMVELTNDGPVTILLDSSKSF"
     gene            12737..13351
                     /locus_tag="PC700_000790"
     CDS             12737..13351
                     /locus_tag="PC700_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2580114.1"
                     /translation="MIIKKFVDSYFGVNTYVLGDEKTKKCAVIDPGGSLVETLSFVKE
                     NELNIEYIILTHGHGDHIGYVKDIKEKTGAKVVAHVDEKELLNDKNKNLSYTMRCGAQ
                     EFDADIYVSDKDKLELGELKMTFLHTPGHTQGCMCIRVEDEMFTGDTLFAGSIGRTDL
                     YSGDFNQMEKSLKKLCKYEDAVRVYPGHGPTSTLGVEKKTNPYM"
     gene            13442..14998
                     /locus_tag="PC700_000791"
     CDS             13442..14998
                     /locus_tag="PC700_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HDF2580115.1"
                     /translation="MLYVFLKGHDYKYEVAELIKLFTSEFKFKDSNANNNIESKHLIN
                     RLIYENGVLFSSTEYYENGNLKYESIQNIENMNLEFLDGFDSSKTIEEFLNRLDSESK
                     RNFKKLCKENIKKSMFVVLKQVFNSYVPWGILTGIRPVKIVHNLMDKKLDDNSIRTIL
                     KDNYFIIDKKIDLALEIAKRERLFMYPIDKNKISLYVSIPFCPTRCVYCSFPSNSLKQ
                     FGHLKREYVYKLIEEIKGFAKVIKETKKEVETLYIGGGTPTTLDEEELDLLINSLFNE
                     LDLSKIKEFTVEAGRPDTITEQKLRVLKKHNVSRISINPQTMNDDTLVKIGREHRVSD
                     LVDCFNMARRMGFDNINMDIILGLIDEELNMVKNTLEEIKKLSPESLTVHTLAIKRAS
                     NLNINMDRYKEHLTQYEEMVKMIDLSMKYAKDMGLNPYYMYRQKHMLGNLENIGYAKE
                     GYECIYNIQIMEEKQSNYALGAGAISKFVYVDEDRIERVENVKNVEQYIERVDEMIKR
                     KKEEVYKNVD"
     gene            14988..16250
                     /locus_tag="PC700_000792"
     CDS             14988..16250
                     /locus_tag="PC700_000792"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDF2580116.1"
                     /translation="MLTKAPRGTKDITPKEAYKWRYVENKFREICALYGYEEMVTPIF
                     EHTELFKRSVGDTTDIVQKEMYSFKDKGDREITLKPEGTAGVVRAFIENKLYADTQPT
                     KLFYVTPCFRYERPQAGRQRQFHQFGIEALGSDTPSMDAEIIALAVQFFNEVGLNDLV
                     VSINSVGCPVCRKEYNALLKEYLDSKADILCDTCNERREKNPMRVIDCKNPTCKENIK
                     DIPFIADHLCDDCKSHFDKLQEYLKEMNINFVIDKTIVRGLDYYRKTAFEIISNDIGA
                     QSTVCGGGRYDGLVEQLGGPKGISGIGFGLGIERLLLTLEGNGIEIENPQSTDIFIVT
                     IGEEANTRSFKLLKDLRQNHISADKDHIERSVKAQFKYSDKINSKFTIVIGDDELKND
                     TATLKNMKTSEQTTVKLSTLVEELKQKL"
     gene            16324..18111
                     /gene="aspS"
                     /locus_tag="PC700_000793"
     CDS             16324..18111
                     /gene="aspS"
                     /locus_tag="PC700_000793"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDF2580117.1"
                     /translation="METLKGLKRTHYCGELREKNINEEVVLMGWVQKKRNLGGLVFVD
                     LRDTSGLCQIVFDTDVDKEAFEKAEKLGAEFVIAVKGKVCERQSKNPNMPTGDIEIFA
                     TELRLLNKSETPPIYIKDDDDVSEALRLKYRYLDLRKPSMQRNLKLRHKVMNITRNYL
                     SNNRFCEIETPFLIAPTPEGARDYLVPSRVNPGKFYALPQSPQLYKQLLMVSGMDRYF
                     QIVKCFRDEDLRADRQPEFTQIDCEMSFVEQEDVMSMIEGLLEAIFKEVLDVELALPL
                     PKMTYAEAMSKYGSDKPDTRFGYELTDISDVVCNCGFKVFADATQPGKSVRGINVKGK
                     ADDFTRKQISSLEEHAKTYRAKGLAWMKVGQEGVTSPIAKFFNEEEMNAILTKMNAEV
                     GDLLLFVADKNSIVFDALGQVRLEVANRLNLLDKNVYNLLWVTEFPVFEEDEETGTFS
                     AMHHPFTSPMDEDLDKLEEGDKSSLRAKAYDIVLNGYEIGGGSVRISNSDIQSRMFKA
                     LGFTEERANEKFGYLLEAFKYGTPPHAGLAFGLDRLVMLLAGTDNIREVIAFPKNQNA
                     VCPMTNAPTLAEDEQLEELSIKVDIKDNE"
     gene            18570..19802
                     /locus_tag="PC700_000794"
     CDS             18570..19802
                     /locus_tag="PC700_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HDF2580118.1"
                     /translation="MQEREELVLNPVPKEERVGWLAPLFNMLGSNIAISELMVGGTLI
                     LGMTLSNMIITSIIGNLILVAIIMIQGYIGYKEGLNTYVLAKGAFGEIGGKYLISLLL
                     GITSFGWFGVQAGVAGLSVQKIFPSVNLTLVTVILGLFMVVFALYGFKAMAKFNYLVI
                     PPLMILMVWGVFKAFSTYGVEAIYNYTPQTTMSMVEGLNIVVGLVIVGAIISPDQLRY
                     TRRVKDIWIISFLGLGIISLFQQVAAGVMSMGAPTWDITEVLANLGFGWVAFVILILA
                     SWSTNVSNAYSGGLALKTIFPNVKRNVLTLVAGLIGTIIAATGIIFKFQSFLSFLGIA
                     VPAIAGIMWCEYYFIQGRTYKHREGINWIAVISWLIGFAASYYSSKINFLIPPINGIV
                     VSMVIYYILMKCFGIKDK"
     gene            19816..20622
                     /locus_tag="PC700_000795"
     CDS             19816..20622
                     /locus_tag="PC700_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HDF2580119.1"
                     /translation="MGRKVKIGIIQQHSVLGDVKKNIEKAVEMIDDLGKHGADIICLP
                     ELFATGYNLESLGGVKTLELIREHNKYIEESMSEAAKRNNVYLISPYGTLEKGSTHVY
                     NSAVIFDRKGKIMGEYCKNHLWSLEAVYFKGGEKVEVYDADFGRFGVMICYDAGFPEV
                     SRELTLKGSEIIFIPSAWRIQDEDMWDLNVSQRALENTVYTVGVNLVSNDSNLILFGK
                     SKICNPRGTVITQLDTYREKYVLSEIDLDEIKKYREDIPYLKDRKSSYNI"
     gene            complement(20734..21603)
                     /locus_tag="PC700_000796"
     CDS             complement(20734..21603)
                     /locus_tag="PC700_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2580120.1"
                     /translation="MDLLHLKYFQTVARMEHITKASHKLNIAQPALSKTISSLEKELG
                     VQLFDRKGRYIVLNEYGRLFLKRVDSILDLVESSKKELQDTSLENSGEVKILSPAAAN
                     VLPSLLSNFRKLYPNITFNVSHTLPSSYKKSDFDLYISSSFTKLNSENSITLTCEEIL
                     LGVSINHPLSLKDEVYLSEVSDENFIVITKGENYREVIDILCESANFKPKIAFESDSP
                     YTIYALIKSLQGVGFICGKSWGLSQDPEIKLLHIKDIEFKRYLNLSWFSENYESKAVL
                     LFKNFLINYFKNI"
     gene            21974..23401
                     /locus_tag="PC700_000797"
     CDS             21974..23401
                     /locus_tag="PC700_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp14"
                     /protein_id="HDF2580121.1"
                     /translation="MKLTKSISSMLCLALVLSLSPVAYANEISRTTVENTLVGQDRYQ
                     TAVEVSKKGWSSANEAVVVNSESIVDALAVTPFAKLKNAPILLTEKNNLNSQTEKELK
                     RLGVKKVYIIGLSSSVSNDVQKQLESNNLTTDRVGGSDRYQTALSIAKKIEGLKDISE
                     IAVVNGYTGLADAVSIASVAATNGMVILPVSDASGISSFKDFIESKNINKSYIIGSTN
                     AVSDKIKQSLPNSERIGGADRNETNGKVIEKFYTSNKLNNVFVAKDGMKNQNQLIDAL
                     AVGVLASKENSPVLIVGEQLSATQKSVFLNKNATSITQVGSGGNENAFKELKDLQANS
                     NNKRVMYVTNTDKVNIRNDATIEASVIGYLNNGDEVEVLDVLKTGWVKIKYNEGIGYV
                     SGSYLTNNKPDNSNENIKIKYVKEKDGLNVRKGPSTEDEKIGHLSYGSKVETIEIFAT
                     GWVKIKYNGGYGYVSNDYLSDTPVI"
     gene            complement(23477..23746)
                     /locus_tag="PC700_000798"
     CDS             complement(23477..23746)
                     /locus_tag="PC700_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580122.1"
                     /translation="MKFEYYYLIQDIAGILLAFIGLRMSIIGFRILSMKGISINTLLI
                     VIKYCLFTIAGLNLLISKFGIRHWIWSVCMLIISIIINPRIKVSK"
     gene            complement(23749..25191)
                     /gene="nhaC"
                     /locus_tag="PC700_000799"
     CDS             complement(23749..25191)
                     /gene="nhaC"
                     /locus_tag="PC700_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDF2580123.1"
                     /translation="MKMNTERKIPSFRYSLLVMLAIIALTSVGIVVFNASITTMFLLS
                     WLIVVPAATKLGYTNDEIEAFGFEVGKDAFQSNLIILSVGVLIAAWIAAGTIPTVVYS
                     GLTIITPKYFLLTTLIVCSLTSVATGTSWGTLGTSGIAMMSIGTSMGIPLGLTAGAVI
                     SGAFFGDKMSPLSDSTNLAAAVCKTDVITHMKHMFYTTGPAYVICIVLYTVIGFKYSN
                     NTIDYVQINQIKDVLNSNFHIGLVAMIPIIFLLLLLLLQKPPIISILSSAIMGLIIAV
                     FQEGEKIGDILNYMLSGFTIDTGFVYADKLLNRGGIMSMAETVLLVFVVFVIAGILQK
                     TGFLEVLLQPLITKIGKSRTKLVGSTFIVSYFANAFSSSMMFTSVFVGTLMSPIYKEF
                     KLKPQNLSRIIEDTATLGGPLIPWNSNAVFCAQTLGVSPLEFIPYCFLSWITPIISFI
                     YGVTGFTMLTYTDDEIEELEIFDTAQRRVD"
     gene            complement(25216..26382)
                     /locus_tag="PC700_000800"
     CDS             complement(25216..26382)
                     /locus_tag="PC700_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2580124.1"
                     /translation="MNYNFNEIVDRSNNFSSKWSEMEKKYGTNDLLPMWVADMDFKAA
                     PCIIDSLKNRLEQEIYGYTTRPDSYNESIVNWLDRRHNWKIKSEWLIYSPGVIPAISL
                     LINELTKANDKIMIQEPVYSPFNSVVKNNNRELIISPLQKLENGNYIMDYEDIENKIK
                     DVKLFILCNPHNPVGRVWTKDELKKLGDICLKHNVKIISDEIHSDIILKKHKHIPMAS
                     ISKEFEKNTITCMAPTKTFNIAGLQSSYVILPDEKDYKLLDDAFTRIDIKRNNCFSLV
                     ATEASYNNGESWLESFLEYLESNIDFAIKYINENMPKLKVRKPEGTYLLWVDFSALGL
                     SDEELESVLVQKGKVALNQGNSFGIGGSGYQRINLACPRSMLEEALIRIKNAIK"
     gene            complement(26607..28727)
                     /locus_tag="PC700_000801"
     CDS             complement(26607..28727)
                     /locus_tag="PC700_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2580125.1"
                     /translation="MKQMELAIVSLKKDAGEIYENQIRQFLGDNLKINLYSFEEGNLK
                     FFKEKLILLSAYLKYDEIVKLSHYDAQIIVPKLTFEKNSIDMISKLEKDKIIYVYNLS
                     KDMAIETISLIHRLGIDNINILPCYPEIEFTPTDAVILTPGEKILPKFKNCEVVDLKY
                     RIIDLSCIVEIATKTKLKHLIKDDLIKKYVEKIIPTSYSTGELLLDANKFERQFDLLL
                     SIIDDGIICTNNDGIIQFYNHIARKILSINANEMIDSFVGDCIKDINFQNILTNKTPF
                     FEKLIKINHIDINLEIKHIQLNVFDGFILKMTKFSQLEKKQAKLRAQLVNSGNISKYT
                     FDDILGSSIQTINTKKIANKMAQSNSSILIIGESGTGKELFAQSIHSASRRKDGPFVA
                     VNCSTFQENLLQSELFGYDEGAFTGAKKGGKIGLFELANNGTIFLDEIGEMDLNSQSK
                     LLRVIQEKQVRRIGSNNVIDIDVRIIAATNRNLKELVSKNMFRRDLYFRLNVLPLKIH
                     PLRERAADIFEIFGSLKYDIPCNFILSEEVKEIFKMYRWEGNVRELRNLGEYFCYLGK
                     DIIEICDLPEYILDTIDSNYSRTVCNKVSDNIKKYQFNIGKDKNIMKYDYNFKRSLDE
                     YIFILDNLKKAYDLKERIGRKSLCKIALEENRFLTEQQIRNMLLELQDFGLVDILVGR
                     GGSIITSKGVEFLKNINRSNKLNS"
     gene            28960..29361
                     /locus_tag="PC700_000802"
     CDS             28960..29361
                     /locus_tag="PC700_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580126.1"
                     /translation="MKKCLYCNRDIKSPNNKLAIKYDDNMNIYPCRAECKEKIEEYIA
                     KKPTYKFINILEVLLGVGGIFCFLSKWTIAAQVVLGIDALVIFLFPLAGFKMNGKLSM
                     EQTKNQSRSIAISILAFIVIITVLYFKQVGK"
     gene            29404..29697
                     /locus_tag="PC700_000803"
     CDS             29404..29697
                     /locus_tag="PC700_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HDF2580127.1"
                     /translation="MEERKLAPNNEREALIKRLKRIEGQVKGIQKMVEQERYCVDILV
                     QISAIRSAINRVGTIILENHIKGCVAESIKHDDADKTEEMIKELMDTINKFTK"
     gene            29712..30152
                     /locus_tag="PC700_000804"
     CDS             29712..30152
                     /locus_tag="PC700_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HDF2580128.1"
                     /translation="MNQQGFIMEIVDDRTAKLKMQRHSACAACGKCATTNSAESKEIL
                     VEVDNTIGAKVGDHVEVSMDNMNVLKAAVMAYIIPLIALLVGTIGTYYILGAIGMTTG
                     VEAISGLVGICLTIICYLYLKKNDKKFRDSREFIPVITRILIDL"
     gene            complement(30244..30717)
                     /locus_tag="PC700_000805"
     CDS             complement(30244..30717)
                     /locus_tag="PC700_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDF2580129.1"
                     /translation="MTDMSMILHFLFSGVATAGFAVFFNAPLYLLLPAGVTGGIGWIV
                     YVYLFNFTTNAVFAGFIAAALVSACSETLARKLKQPAIVFVIPGILPLIPGIGLYNTM
                     LSLIQKNYSLAMSKGTDALFLSAAIALGVLVVTSFVRTLNLLKIRKNFIPYRKSK"
     gene            complement(30717..31505)
                     /locus_tag="PC700_000806"
     CDS             complement(30717..31505)
                     /locus_tag="PC700_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDF2580130.1"
                     /translation="MNQNEELEYKKNILKLALFVGELMIKNGAETSRVEDSVLRICRS
                     RGFYHVNIFTTPTVIIISDEKFDGFSFMKTIQSRGINLNKISLLNSFSREFVSDKEYD
                     IDNAIARLYEIQDVKPYPLWLFYSCTGIASACFACLLGGNYVLNFILTFIIAIFAAIT
                     YDKTMKISAISAFSCLVSSFLIALSGVLLVEIGILDDPKMLIVGAIMPLLPGVAFIKA
                     IRDLISGNLISGISRAFDAAMIIIAIASGVGFVLDTWYRIGGPF"
BASE COUNT        12311 a         3614 c         5390 g        10262 t
ORIGIN      
        1 atatagtaaa taagaaaaaa tttgaaaaaa atcactataa atgagtaata aatataaata
       61 gtgtatacta atgggatgga aaatgtccac tattaacttt attaccccac atatataaga
      121 taaaattatt aatataaaat ataatctatc gacaatgaat agacttttaa ataaacttaa
      181 tataaaatta attaaataag tgcttaaaaa cgatttactt taatgataga atcatattaa
      241 aggaggataa ttttatggat ataaagaata aaattaaatc tatattttta ccagaaaaaa
      301 atgactattt agatgatgaa gaatttgagt atgatataga aaataatgaa gaagatttaa
      361 aggaagataa caaagtacat ttaaatgatt taagcagagt aaaatatgat tacagagagt
      421 taaaagacat agaaaaccaa acagaagaga tgtgtctgct agcagtaaag caggatggta
      481 ctataattga gtttgtaaag gacaagactt ataaagtatg tatggaagca gtaaaacaga
      541 catataaagc tcttaagtat ataacaaatc aaaatgagga tatttgtata gaagcagtaa
      601 aacagaacta cagagctttg tactacataa ataataagac agaaaatgta ctaattgagg
      661 ctataaaaaa tgcatcaact tatgatgtca tggaagtatt taaatttgtg gaagatcaaa
      721 cagaagatgt atgtctagca tttatagaaa gggcatctaa aaatgatgtt gcagagatat
      781 taaaaggaat aaaagagcaa actccagcca tatgtttaga agctgtaaaa aaagatggaa
      841 aatctttagc ttatgtaaag gaacaaagta atagtatatg tttagaagct gtaaaagaaa
      901 actacagtgc actatcttgt gtaaaagagc aaacagaaga aatatgtata gaagcagtaa
      961 aacagaatga ctttgcattg tattatgtaa atgaacagac agaaaaaata tgtatggaag
     1021 cagtaaaaag aagttatatg gctctacagt atgtaaataa gcaaacagaa gagatttgtt
     1081 tggaagcagt aagaattgat ggaagagcat tacagtacgt aaaagagcaa acagaagaaa
     1141 tttgcttgga gtcagttaga caaaatggaa aagtattgca atatgtaaaa aaacagactg
     1201 aaaatatatg tatagaagca gtaagaggtt catttgaaga acttgaaata aaagaaatat
     1261 taagttatgt aaagatacca acagaaagaa tatttgtaga agcagtaaag caaaatggaa
     1321 aaattcttaa atatgtagaa aatcagacag aattaatatg tttagaggca gtaagagaaa
     1381 actataatgc tttagcttat gtaaaggaac aaacagaaga gatttgttta gaagcagtta
     1441 atcaaagtta tgaagcgctt aaatatgtga aagaacaaac agaagaagtt tgtctaaaag
     1501 cagtaaaaca agattataga atgttgaaat atgtaaataa tcaaacagaa aaaatatgtt
     1561 tagaagcagt aaagcaaaat tatagagctt tagaatttgt tgataatcaa acagaaaaag
     1621 tttgtcttga agcagtaaaa cagaatagaa aagcactaca gtatgtaaaa caaaaacaaa
     1681 gttaaaaggc taaaatatat agaaaatata tgcaaataaa taaaatttga tattttgtaa
     1741 taaataaaat gggtatctca aaatgaactt tttagtttgt gaggtactct ttttatatga
     1801 aataaaatat atttttagcc ttttaacaat ggggaaaagg tttatttcta ttttgacata
     1861 ggctttttat gcatataaag cttgataaaa ttgttttatt ataattgttt tgttatataa
     1921 ataataataa gaaaattata taggtctttt tttgaccagt tttgcatttt taacaactag
     1981 ttttgtaaag tatattgaat tttctcaaaa aagaatgtac tctatatatg taaaattttt
     2041 ttggaggtaa aaatgtttaa aagatatgct gaaaaaatga catctgtttt aatttgtaat
     2101 aatatgatag ataataatga atctaaagtc tattcttatg gatttgaaat attaatagct
     2161 ttcatagtta atataaccac catgctgttt ataggttttt tatttggtaa atttacatat
     2221 gtattatttt ttttaatgtg ttattgtcca ataagacaat tttcaggagg gtatcatgca
     2281 gacaattatt ttagatgctt attaaccttt atttttataa ttttatctac aatcttaatt
     2341 atagaaaata taaatataga cttatttaaa aatattatta tgattattgc aagtgttagt
     2401 tgggttggaa tttgtgttct gtgtccaata gaacatagga gtaaccctat aagtgatagg
     2461 gaaaagtttg tttataagaa aactgctata tttatatcta cagtagtgtt attgataaca
     2521 attttaagtc tttctttgag tatatttgta gattatttca catatagcgc atttgccatg
     2581 ttttggattt ttgtgatgtt agtattaggt aagttaaaag ctaaagtata agtaaaatat
     2641 gtatgaggga gagttaagtt atgaaaaagt ttattgtaag atttatgaag tttgctagct
     2701 cattggcact tagcactgct attttgtcag ctaatagtac ctgtccttgg attattcatc
     2761 agcctaaagt acccaaagaa atcagcaatc taaagaagac aaattaaata caagcactta
     2821 tttatacaaa attatcagga aaaaagaaat tattatattt taataagatt aaaacaatat
     2881 aaaatcagat acaaaattta ttttatttat tttataggag cctaatacct atttggtaat
     2941 atattagtgc gccaatttag ggggatatag tgtatttaat gaaatgactt acggagagta
     3001 agtcattttt gtattgtatg tattttatgt aaacttatga tataatgaag ggtaaagttt
     3061 aattaaaata ataaaaaact ttagtataga tagtaattta ggaggtaggg tatgagtatg
     3121 atacataaat tttctatgaa tggatataat atcgttttag atgtgaatgg tggagctgtt
     3181 catgtgttag atgatgttgc atacgatttg ttagattttt ataaagaaaa aagtaaagaa
     3241 gaaatattag agatattaaa atcaaaatat caagaagaaa aaataaatga agcatatgaa
     3301 gaaatattaa atttggaaaa agaaggtctt ttatacacag aagatacata tcaatatcat
     3361 ccaagctttg tacatagaga gcctgtagta aaagctttat gtttaaatgt agctcatgat
     3421 tgtaacttaa aatgtaaata ctgttttgca gctcaaggtg attttggagg agaaaaagaa
     3481 cttatgagtt ttgaagttgg taaggcagca attgactatt tgattgctaa ttcaggaagt
     3541 agaaagaacc tagagataga tttctttggt ggagagcctt tgatgaactt tgaagttgta
     3601 aaacaactgg ttgactatgg aagaagtgta gaaaaagatt ataataaaaa tataagattt
     3661 acaataacta caaatggtgt actgttaaat gatgagatta tagattatat aaatgaaaat
     3721 atgcataatg tagttttaag tttagatgga agaaaagaag ttaatgataa tatgagacca
     3781 actttaaatg ataaaggaag ttatgatatt acattaccac gatttaagaa acttgtggaa
     3841 aaaagagcaa aagataagta ttattatata agaggtactt ttacaagaga taatttagat
     3901 ttttcaaaag atgttatgca ttttgcagat ttaggattta aattaacctc tgtagagcca
     3961 gtcgttggag atgagagtaa tccatatgca cttagagaag aagatttacc taagatattt
     4021 gaagagtacg aaaaatttgc tgtagagtat gcagatagac aattacaagg agatggattt
     4081 aaatttttcc attttatgat agacctaaat caaggtccat gtgtaataaa gagaataact
     4141 ggatgtggag ctggaaatga gtatctttct gtaactccaa atggagatat ctatccatgt
     4201 catcaatttg ttggaaatga agaatttaaa atggcaaata tatttgatga agaaatagta
     4261 cttccagaaa atttaaaaaa tatgtttaga gaagctcatg tatatactaa agaagagtgt
     4321 aaacaatgtt ggaataagtt ttactgttca ggtggatgtc atgcaaatgc gataaacttt
     4381 aataatgaca tatcaaaacc atatgaatta ggatgtgaaa tgcaaagaaa acgtacagag
     4441 tgctctataa tgatacaagc taagttaatg ttagaaggag caaccaacta aatttatatt
     4501 ttaaaggagt tgtttatata tgattagaga ttatttagaa gataaacctt taattgacga
     4561 aagtgtattt gtggcaaaat cagcagatgt aattggaaat gttaaaatag gcaaagattc
     4621 aagtatatgg tacaatgcag tagttagggg tgatgaagga ccaataacta ttggagaaaa
     4681 cactaatata caagattgtt ctatagtgca tggagataca gaaactataa ttggtaacaa
     4741 tgtaacagtt gggcataggt ctatagttca tggttgtaaa attagtgata atgtgttaat
     4801 tggtatgggt tcaataatat tggataatgc tgaaatagga gaatatactc tgataggagc
     4861 aggtacacta attacatcta ataagaagtt tccaccaggt gttttgataa tgggttctcc
     4921 tggtaaggtt gttagagagc ttacagaaga agataaaaaa tatattgatg aatcttatga
     4981 atggtattta gaggcagcac aaaatcaaaa atattagttt aaagagtggt tgtttcaaaa
     5041 catatttaaa tctgttttga ggcaaccact aatttatttt attagatttt gttgatattt
     5101 tagttttcaa aaagtatttg attaaaccag atataaaatg tcaaaaataa aaattaactg
     5161 agttaaataa ttttaataaa attggtagta tatgtgataa aattaaatat taaagaggat
     5221 ataggatatt agaatatcaa ttggtgaaat aatgtcatat acgataaatt aaggtgtaag
     5281 tttttgggta tacctatctt aggagtgaaa agatttgaga ataagttata gaaatttaaa
     5341 gcgatataga gaaattggat atgttttaat aaagtatggt tttagtttta ttgtagaaag
     5401 actaaatata gaaggaattg cctataaaat accactattt gacccaccag aagaaataaa
     5461 aaatatgaca actggtgaga gaatgaaaag agttttagaa gaactaggac ctacatatat
     5521 aaaaatagga cagatattga gtacaagaaa agacttgtta gaccaggata taattgatga
     5581 aatatcaaaa ctcagagatg atgtagagaa atttgattca aatatagcta ttgatatatt
     5641 taaagaagaa gtgggtttaa gtatagaaga aatattctta gaatttaaag aagaaccaat
     5701 agcagcagct tcaattggtc aagtttatga aggtgtactt aaaacgggag aagaagtaat
     5761 cgtaaaaatt caaagaccaa atatagagaa aataataaaa tctgatttag agattcttag
     5821 aactattgca aatactttaa aagatttaaa aaaggatttt aatttagatt tagtacagat
     5881 gattgaagaa tttcaaactc aattaatgag ggaacttgat tatacatttg aagccataaa
     5941 tgcaactaaa ttctctagga tatttaaaaa tagtgatgaa gtatatatac ctaaagtata
     6001 ctcagaatat aatactaaga aaattttagt aatggagaaa gttaatggta caaagttaag
     6061 tgatgtagaa aaaataagga gactaggtta taacacaaaa actattgttg aaataggagt
     6121 taggtcattt tttacacagg tattatctca tggatttttt catgcagacc cacatccagg
     6181 taatatattt gtagttgcta aaaataagat agcatatata gattttggaa tgattggaat
     6241 tattgataat aaaactttaa accaattaaa tgaaattgca ttagcaggtg tagaaaagaa
     6301 tgttgacaag ataatatatc tattgataga aatggatgca ttaaatggag aggctgatat
     6361 taagggactt agacaggatt tactttatct aatacattat tactatgata tttctataga
     6421 aaaaataaat gtaacagata tattaaatga actatttagg ttttttagac agtataaaat
     6481 agtcatgcct gcacaatttg taactcttgc aaagacagtt ataacattag aaggaacaag
     6541 tagaacttta aacacagatt tttcttttgg ttctatggga aaagagttta tgaaacatca
     6601 ttataagagt aaatttaacc ctaaaaatgt tgttttaagt tcaagacaaa atgtagaaga
     6661 aatattactg gacataaaaa ctattccaaa gcaattaaaa gcaattttaa aaaatataga
     6721 aagaaataat ataaaaatgc aaattgaaga tgttaaaatg acgaggttag aaaattgtat
     6781 aatagagcta acatctcaaa tatctttgag tttagtgttg gcatctatta tagtaggttc
     6841 ttctcttata attgcatctc caaatataga aaataatata tggataaaat ttactgcaat
     6901 agcgggtttc tttatatcct ttataatagg cctttgctta gttataagaa gtattaggtc
     6961 aaaatacaaa aaagattagg gggaattaaa tttgaaaaaa tggatattga agtataaagg
     7021 taagatagag gaaagtgaat ttagcaaaaa acttaatatt tccccggaaa tctgccaaat
     7081 tttaaaaaat agagggatat gtactgaaaa agattcagaa atatttatga atccatcttt
     7141 agattattta agagaccctt ttttaatgaa agatatgcaa aaagctgttg atagaataaa
     7201 gttagcagtt gagaagaacg aaaaaatttg gatttacgga gattatgatg ttgatggtgt
     7261 atcatctaca tccatcttgt gtttatattt taagagtata ggttacaatg ttgattacta
     7321 tattcctaat agattagaag aagggtatgg tataaatgag gatgctatta agcttataag
     7381 ctctagggga tgtgacttga ttataagtgt tgactgtgga attacatcag tgtctgaggt
     7441 caatacagcg aatgatttgg gcatagatgt tataattaca gaccatcatg aatgtcaaag
     7501 tgaaattcca tcagcctttg cagtaataaa tccaaaacaa gaagattgta attatccatt
     7561 tgattcacta tgtggatgtg gtgtagcatt taaaatgatt caagccttga ctccaaaaga
     7621 agaatttaaa actagtatgt ataattattt ggaaatagta acactagcaa ctatttgtga
     7681 tattgttcca cttatagatg aaaatagaat aattgtaaaa aatggtttaa aatcaatgaa
     7741 agaaggcaaa aatattggat taagggagct tataaaagtt tgtggtgtag aaagtgataa
     7801 gataggttct tctcatatag gttttgcaat aggtcctagg ataaatgctt caggtcgatt
     7861 gggatattct tacttaggag tagagctatt tactactcag tcacaagaag aggcagtaga
     7921 aatagctagt atattggaag aaaaaaataa tgaaagacag atgatagaag ctaagatgta
     7981 tcatgaagct gaagaaatgc taaagtctaa tagcagatac aatgatgaca aagtgttggt
     8041 tttagccaaa gagggttggc aacatggaat aataggaatt gtagcatcaa agttaactga
     8101 aaaatactac aaaccaacaa ttcttctagg tatagaaaat ggggaggcta ctggttcagc
     8161 gaggtcaata aaaggattta atatatttga agctctcata aagtgcaaag acttgatgac
     8221 taagtttggt ggtcatgaac aagcagcagg gttatccttg gattcagaca atgtagaaat
     8281 tttagcaaat gaaataaata aatttgcaga ttataattta actgaagatg atatgataga
     8341 gaatgtaaat gtcgagtttg aattacaaga aaatgtaata aatttaaatc ttgtagaaga
     8401 attacataag ttagaaccct ttggattaaa taatcctaat cctagattta tagttagaaa
     8461 ttatatttta aaagatttga aagttatagg taagaatcaa caacatctga aattaagtat
     8521 agaaaaagag aaaagctatg aatgtatagg atttaatatg agtcatttaa aatcaatgta
     8581 taaagtagga gacaaagtag atgtactatt ccaattagat gaaaataact atatgggaaa
     8641 taggaaagta caatttttac ttaaagatat acgattggct cgacctaaaa gtgcaagtaa
     8701 tgacaaactt tcattgaaat taatgtcaaa aataatacca aaagatactc aaagcctgta
     8761 taatatatct gtgtctgatt ttgaattatt tgatggaaat acagatataa atatatttga
     8821 ttattttgaa aaagatacac taataatatc taattctata aatggatttt atagagcaat
     8881 gtcagatata tcacttatag atttggattt taacataaat tacaatatta ttgaagatgt
     8941 tagcaaaaat actgataaat tagagcttat attttctcct aatattgata aaatagactt
     9001 aaaaagatat aataatatta ttctttatga ttatttatac aataaaggcg aatattcgta
     9061 tatatatgag aataaaagag aagaaagtga aattataaaa tattataata aaacagacct
     9121 attatattta aagaatgtag ttagtaatat agttccatca agagatgaat ttataacaat
     9181 ttataaacaa gcacttataa aaaaagaaat tgatttggat atggtaaata taagggaaac
     9241 ttttaatgta attccattaa agttttttac gatattaaat gtatttagag aattaaattt
     9301 acttgacttt aatttaaatt atgaaaaaaa ttctgtactc ataagaatat tgccaaaacc
     9361 acagaaaaaa ttagacttaa atgagagttt aatactcaat aatctaaaaa atttagaaaa
     9421 acaatacaat agcagttatt aggaggcgtt tcccatggat ttaaaaaact ttataaggaa
     9481 tattgatgat ttcccaaaac caggcataga ttttaaagat gtgacaactc tatttaaaga
     9541 tggagatgca tttaaatatg cagtggattc tatagttgaa gaattgaaag ataaagatgt
     9601 agatttagta atagggccag aagcaagagg atttttgatg ggtacacctg tagcatatgc
     9661 tttaggagta ggatttgttc caattagaaa acctggaaag ttaccaggtg aagttgagag
     9721 ttatgagtat ggattagagt atggtacaga tactttagag atacataagg atgcaattaa
     9781 aaagggtcaa aaagtagcaa tagtagatga tttattggct actggtggga ctatggaagc
     9841 cgctgccaag cttgtagaaa aattaggtgg agaagtagtt tctatgcaat ttttgattga
     9901 gctaaaattc ttaaatggga gagaaaagtt gtctaactat gatgttaatt ctcttataaa
     9961 atactagtac ttttataata gttggtataa caccaactat tttcatatat attaaaattt
    10021 tatacaaaat aggtggttat atgcatgata aagaattaca agaaataatt gaaaaaataa
    10081 aagtttatgc acctaatgca aattttgaaa tggttgaaaa agcctataat cttgcaaaaa
    10141 aagcgcatga aggacaatat agaaaatctg gggaaccata ctttattcat ccattagcag
    10201 ttgcaaatat tttaatagag atggaattag atattgaaac tataacagca ggtttacttc
    10261 atgatgttgt agaagataca gaatatacct atgaagacat aaaaaaagaa tttggcaaag
    10321 aagtagcgga tttggttgat ggagtaacta aacttggtca aataaaatat cgttcgaaag
    10381 aagaaactca atctgaaaat ttaagaaaaa tgtttttggc aatggcgaag gatataaggg
    10441 ttatacttat aaaacttgct gatagattgc acaacatgag aacattaaaa tacatgcctc
    10501 cagaaaaagc aaagtataaa gcaaaagaga cacttgaaat ttatggtggt atagctcata
    10561 gattaggaat ttccaagata aagtgggaac tagaagacag agcattaaga tttatggacc
    10621 cagaagggta ttatgattta gttagtagag tatcaatgaa gagaagtcaa agagaggatt
    10681 atattcaagg aattgttgaa cttcttaaag ataagtttaa agatttaagt ataccatgtg
    10741 atgtatatgg tagaccaaag catttttaca gtatatatag aaagatgcaa aaaaaacata
    10801 agacttttga agagattttt gatttaacag cagtaaggat attggttgat aatgtaaagg
    10861 attgctatgc agtacttggt atagttcata cattatggag acctattcca ggaagattta
    10921 aagattatat agcaatgcca aaacccaata tgtatcaatc attacacacc actgttgtag
    10981 gtcctgatgg agaaccttta gagatacaaa taagaactca tgagatgcat aatatagctg
    11041 aaaatggtat agcagcccat tggaaatata aagaaggtat ctctagcaaa gagtcaaagg
    11101 ttgaagaaaa acttcaatgg ttgagacaga tgatggagtg ggaaaaagat ttaaaagacc
    11161 cacaagagtt tatggatgca cttaaagaag atgtatttaa tagtcaagtt tatgtattta
    11221 cgccaaaagg agacgtaatt gaattaccag cagaatcaac acctatagat tttgcctata
    11281 gagttcatac aaatgtaggt aataaatgtg taggagctaa aattgatgga agaatagttc
    11341 ctattgatta taagcttcaa aatggtaata ttgtcgaggt acttacatca tctaattcta
    11401 atggaccaag tagggattgg cttaatatag tgaagacccc aaatgcaaaa agtagaatta
    11461 gacaattttt caagaaggaa agaagagaag aaaatgttga ccgtggaaat acaatattag
    11521 aaagagaatt taagaagcat gatataccac aaaaagaccc tcttgttgaa aaatatatgg
    11581 cattagttgc taagaaattt aatcagccaa gtgttgagga cttaattgcc actgtaggtt
    11641 atggcggtat aatgtcatct caagtggtat ctaaggtaaa agacctatat ttaaaagagg
    11701 ttaaaaaagc taaaaaggaa caacagatac aagaagaaga tttaaataaa cacaatataa
    11761 gtgaaacaga atacagtaaa aagagaaaga aaaacgcatc acaaggaatt atagtaaaag
    11821 ggcttgacaa tatcttagta agatttgcta agtgttgtaa tcctcttcca ggagatgaaa
    11881 ttgtaggata tattactaag ggtaggggag tagccataca cagagctgat tgcccaaatg
    11941 caaacaataa tggtgatttc ttccaaaata gattggtgga ggtttcatgg agttcagcag
    12001 gaaaaggtaa gtttgaagcg gaaattcaaa taaaagctat tgacagaaga ggaattgtta
    12061 atgaaataac tcatattgtt gcaatggata aacttagttt aaacggaata aatgcaagaa
    12121 aaggtaagga tagtattgta agtataaact tacttgtaga ggttaatgac acagaggaat
    12181 taaaaattct aatgaaaaaa ttaaaagcaa ttcctggtgt agaaagtatt tatagaatga
    12241 caaattagag ttaaaataat agggaggaaa ctatgagagc agtagtgcaa agagtatctt
    12301 catctaaagt tacagtagat gaaaacacta taggtcaaat aaataagggg ttactagtat
    12361 tgttaggagt gactcatgat gataaatcaa gtgatgtaga ttatatgatt gataaaatat
    12421 tgaatctgag aatttttgaa gatgaaaatg acaagatgaa tttatctcta atggatatag
    12481 gtggagagct tttagtagta tctcaattta ctttatatgg agattgcaga aaaggaagaa
    12541 gacctggatt tagtaatgca tctagaccag aacttgcaaa taacctttat gaagaatttg
    12601 taaaaaaagc aaaagataaa ggtgtgacag ttggtacagg tcaatttgca gctcatatga
    12661 tggtagagtt aactaatgat ggtccagtaa caatactatt agactcaagt aaatcttttt
    12721 aagggggaaa actttaatga ttataaaaaa atttgtagat agttattttg gagtaaatac
    12781 ttatgtatta ggtgatgaaa agactaaaaa atgtgcagtt atagacccag gtggaagttt
    12841 agtagagaca ttaagttttg taaaagaaaa tgaattaaat attgaatata ttatccttac
    12901 tcatgggcat ggagaccata taggatatgt taaggatatt aaagaaaaaa ctggtgctaa
    12961 agtagttgca catgttgatg aaaaagaatt attaaatgat aaaaataaaa atctaagtta
    13021 tacaatgaga tgtggagcgc aagagtttga tgcagatatc tatgtgagtg ataaagacaa
    13081 gcttgaactt ggtgaattaa aaatgacttt cttgcatact ccaggacata ctcaaggatg
    13141 tatgtgtata agagttgaag atgaaatgtt cacaggagat actttgtttg ctggaagtat
    13201 tggtagaact gatttatata gtggagactt taaccaaatg gaaaaatcac ttaagaaatt
    13261 atgcaaatat gaagatgctg ttagagtata tcctggacat ggaccaacca gtactctagg
    13321 ggttgaaaag aagacaaatc cttacatgta attttatatt agtatttaaa aaagtataaa
    13381 atatttaata actatattag tgagggggtt cttctactgt taaatagaag gagttaagtt
    13441 gatgttgtat gtttttttaa aaggacatga ttataagtat gaagtagcag aacttataaa
    13501 attgtttacc agtgaattta agtttaaaga ctctaatgct aacaataata tagagtcaaa
    13561 gcatttaata aatagattga tttatgagaa tggtgtatta ttctcaagta ctgaatacta
    13621 tgaaaatgga aatttaaagt acgagtcaat acagaatata gaaaatatga atcttgaatt
    13681 tttagatgga tttgatagta gcaaaacgat tgaagaattt cttaatagac tagatagtga
    13741 aagtaagagg aactttaaaa agctgtgtaa agaaaatatc aaaaaaagca tgtttgtagt
    13801 gcttaaacaa gtatttaatt cttatgtacc atggggaatt ttgactggta ttagaccagt
    13861 caaaattgtc cacaatttaa tggataaaaa gctagatgat aatagcataa gaaccattct
    13921 aaaagataat tattttataa tagataagaa aatagattta gctcttgaaa tagctaaaag
    13981 agagcgatta tttatgtatc ctatagataa aaataaaatt tctctttatg taagtattcc
    14041 attttgtcca actagatgtg tatattgttc atttccttca aattcattaa aacaatttgg
    14101 acatttaaaa agagaatatg tttataaatt gattgaagaa attaaggggt ttgccaaagt
    14161 tattaaggag actaagaaag aagttgaaac tctatatatt ggtggtggaa ctccaactac
    14221 acttgatgag gaagaattag atttgcttat aaattcattg ttcaatgaat tagatttaag
    14281 taaaattaaa gaatttactg tagaagcagg tagacctgac actattacag aacaaaaact
    14341 aagagtgtta aaaaaacaca atgttagtag aattagtata aatcctcaaa ctatgaatga
    14401 tgataccttg gtaaagattg gaagagagca tagagtatct gatttagtag attgctttaa
    14461 tatggctcgt agaatgggat ttgataatat aaatatggat ataattttag gtcttataga
    14521 tgaagaatta aatatggtaa agaacacatt ggaggagata aaaaaacttt ctcctgaaag
    14581 ccttacagtt catacattag ccataaagag agcatccaac ttaaatataa atatggatag
    14641 gtataaagaa catctaacac aatatgaaga aatggtaaaa atgatagatt tatcaatgaa
    14701 atatgcaaaa gatatgggat taaatccata ttatatgtac agacaaaaac atatgttggg
    14761 aaatttagaa aatataggtt atgctaaaga aggatatgag tgcatttata atatacagat
    14821 tatggaagaa aaacaaagta actatgcatt aggtgcaggt gctatatcga aatttgttta
    14881 tgtagatgaa gatagaattg aaagagtaga aaatgtaaaa aatgttgaac aatacattga
    14941 aagagtagat gaaatgataa aaaggaagaa agaagaggta tataaaaatg ttgactaaag
    15001 ctcctagagg aactaaagac ataactccaa aagaggctta caaatggcgt tatgtagaaa
    15061 ataaatttag agaaatatgt gctttatatg gatatgaaga aatggtaaca cctatatttg
    15121 aacatacaga acttttcaag agaagtgttg gagatactac agatatagtt caaaaagaaa
    15181 tgtattcttt taaagataaa ggtgatagag aaattacctt aaagccagaa ggtacagcag
    15241 gtgtagtaag agcatttata gaaaataaat tatatgcaga tactcaacca acaaaattat
    15301 tttatgtgac accttgtttt agatatgaaa gaccacaagc tggtagacaa agacaatttc
    15361 accaatttgg aatagaagca cttggaagtg atactccttc aatggatgct gaaataatcg
    15421 cactagctgt tcaatttttt aatgaagttg gattaaatga tttggtagtg agtataaatt
    15481 cagttggctg tccagtttgt agaaaagagt acaatgcttt attaaaagag tatcttgact
    15541 caaaagctga tatattgtgt gatacttgca atgagagaag agaaaaaaat ccaatgaggg
    15601 ttatagactg taaaaatcct acatgtaaag aaaatatcaa agatatacca tttattgcag
    15661 accatttgtg tgatgattgt aaatctcact ttgataaact tcaagaatac ttaaaggaaa
    15721 tgaatataaa ctttgtaatt gataaaacaa tagttagagg cttagattac tatagaaaaa
    15781 ctgcttttga aattatatct aatgatatag gagctcaaag tactgtatgt ggtggaggaa
    15841 gatatgatgg tcttgttgaa caattaggag gaccaaaagg tataagtgga attgggtttg
    15901 gactaggaat tgaaagactt ttacttacac ttgaaggtaa tggaattgaa atagaaaacc
    15961 ctcaatctac ggatatattt atagtaacta taggtgaaga agcaaataca agaagcttta
    16021 aattattaaa agacttgaga caaaatcata taagtgcaga taaagaccat atagagagaa
    16081 gtgttaaagc acaatttaaa tattcagata aaataaattc aaaatttaca atagtgattg
    16141 gagatgatga gcttaaaaat gacactgcga cattaaaaaa tatgaaaaca tcagagcaaa
    16201 ctactgtaaa attaagtaca ttagttgaag aattaaagca aaagctgtaa aataagaaag
    16261 tatgatgttt tgtgtattag ataaatatat agcattaaaa ttaaataaaa tggaggtagt
    16321 atcttggaaa ctttaaaggg tttaaagaga actcactact gtggagagtt gagagaaaaa
    16381 aacataaatg aagaagtcgt acttatggga tgggtacaga aaaaaagaaa tttaggtggt
    16441 cttgtatttg ttgatttaag agatacaagt ggattatgtc aaatagtttt tgatacagat
    16501 gtagataaag aagcttttga aaaagctgaa aaattagggg ctgaatttgt aatagctgta
    16561 aaaggaaaag tatgcgaaag acaatctaaa aaccctaata tgccaactgg tgatatagaa
    16621 atatttgcaa ctgaacttag acttttaaac aagtcagaaa ctccgcctat ttatataaaa
    16681 gatgatgatg atgtttctga agcactaaga ttaaaatata gatacttaga tttaagaaaa
    16741 ccatctatgc aaagaaattt aaaattaaga cataaagtaa tgaatattac aagaaattac
    16801 ttatcaaaca atagattttg cgaaatagaa acaccatttt taatagctcc aacaccagaa
    16861 ggagcaagag attatctagt accaagtaga gttaatccag gaaaatttta cgcattacca
    16921 caatcaccac aattatataa acaattattg atggtaagtg gtatggatag gtatttccaa
    16981 atagttaaat gctttagaga tgaagattta agagcagata gacaaccaga gtttacacaa
    17041 atagactgtg aaatgtcttt tgtcgagcaa gaagatgtta tgagtatgat tgaaggtttg
    17101 cttgaagcga tatttaaaga agttttagat gtagaattgg cacttccact tcctaagatg
    17161 acttatgcag aagctatgtc taaatatggt tcagataaac cagatactag atttggatat
    17221 gagttaacag atatatctga tgttgtttgt aactgtggat ttaaagtgtt tgctgatgct
    17281 acacaaccag gaaaaagtgt tagaggtata aacgtaaaag gcaaagctga cgactttact
    17341 agaaaacaaa tatcatcact agaagaacat gctaagacat atagagcaaa aggtcttgct
    17401 tggatgaaag taggacaaga aggagtaact tctccaatag caaaattctt taatgaagaa
    17461 gaaatgaacg ctatacttac aaaaatgaat gctgaagtag gagatttact tttatttgta
    17521 gctgataaaa actcaatagt atttgatgct ttaggacaag ttagacttga agtagcaaat
    17581 aggcttaatc tattggataa aaatgtatat aacttactat gggtaactga gttcccagtt
    17641 tttgaagaag atgaagaaac aggcacattt tcagcaatgc atcacccatt tacatcacct
    17701 atggatgaag atttagacaa gttagaagaa ggtgataagt catctttaag agcgaaagct
    17761 tatgatattg tattgaatgg ttatgaaata ggtggaggaa gtgttagaat atcaaattct
    17821 gatattcaat caagaatgtt taaagcactt ggtttcacag aggaaagagc taatgaaaag
    17881 tttggttatt tattagaagc atttaaatat ggtactcctc ctcatgctgg tctagctttt
    17941 ggtcttgata gacttgttat gttacttgca ggaactgata atataagaga agttattgca
    18001 ttccctaaaa atcaaaatgc tgtttgccct atgacaaatg ctcctacatt ggcggaagat
    18061 gagcaattag aagaattaag tataaaagtt gatataaaag ataatgaata atatgtagtt
    18121 gcaagaaact aaaatgtaaa aagtctggat ataaagtata tttatattca gacttttttt
    18181 attagaatta ttttatattt tttctctata tattttcata aattatcctt gtattctcag
    18241 ccaatataat taaaatacta tgtttaaaaa ttttacataa acttgattgt tttagaaatt
    18301 acttctgtaa tgagtacacg attttaatta aagaatatat aacaaatttg aaataagtct
    18361 aatatatgac ttaatagatt aaattaaaat aataaatttg gactatttat ctatttataa
    18421 cttaaattat atcaaattga taacaaacta gatattatac ttttttgtag aatagaatta
    18481 caattatata aagaaaaatt ctattaatag tgaaaaaaaa gtagaaaaaa gtagaaatta
    18541 ttcttttata aagaaaggga gggataatat tgcaagagag agaagaatta gttttaaatc
    18601 cagttccaaa agaggaaaga gttggatggt tagctccatt gtttaatatg cttggttcta
    18661 acatagctat atctgaacta atggttggag gaactttaat tttaggaatg actctttcta
    18721 atatgattat tacatcaatt atagggaact taatattagt tgcaatcatt atgatacaag
    18781 gttatatagg ttacaaagaa gggttaaata cgtatgtatt agctaagggg gcatttggag
    18841 agataggagg aaagtatctt atatctctat tacttggtat aactagtttt ggatggtttg
    18901 gtgtacaagc aggggttgca ggactatcag tacaaaagat attcccaagt gtaaacctaa
    18961 cattagttac agttatactt ggattattta tggttgtttt tgcactgtat ggctttaaag
    19021 ctatggctaa atttaattac ttagttatac caccacttat gatattaatg gtgtggggag
    19081 tttttaaagc tttttctact tatggagttg aagctattta taattatact cctcaaacaa
    19141 ctatgagtat ggttgaaggt cttaatattg tagtaggtct tgttatagtt ggtgcaatta
    19201 tatcacctga ccagttaaga tatacgagac gagtaaaaga tatttggatt atcagttttt
    19261 taggtctagg gattatttca ttattccaac aagttgcagc tggtgtaatg tctatgggag
    19321 cacctacttg ggatataaca gaagtattgg ctaatttagg ttttggatgg gtagcgtttg
    19381 ttattttaat acttgcatct tggtcaacaa acgtatcaaa tgcttattct ggtggattag
    19441 cattaaaaac tatttttcca aatgtaaaaa gaaatgtttt gactttagtt gcaggtctaa
    19501 taggtacaat aatagcagct actggtataa tatttaaatt tcagtcattt ttatctttct
    19561 tgggaatcgc agttcctgca atagcaggaa ttatgtggtg tgagtattat tttatacaag
    19621 gaagaactta taagcataga gaaggaataa attggattgc agtaataagc tggttaatag
    19681 gttttgcagc gtcgtattat agttcaaaaa taaatttctt aatacctccg ataaatggaa
    19741 ttgtagtgag tatggttatt tattatatat taatgaaatg ttttggaatc aaagataaat
    19801 aaagggggca attacatggg tcgtaaggtg aaaataggta taattcaaca acacagtgta
    19861 cttggagatg ttaaaaaaaa tatagaaaaa gcagtagaga tgatagatga cttaggaaaa
    19921 catggggcag atataatttg tttaccagaa ctatttgcta caggatataa tttagagtct
    19981 ttaggtggag ttaaaacatt agaattaata agagaacata ataagtacat agaggaatca
    20041 atgtcagaag ctgctaaaag aaataatgta tacctgatat ctccatatgg aaccttagaa
    20101 aaagggtcta cacatgttta taattcagca gttatatttg atagaaaagg taagattatg
    20161 ggagaatact gtaaaaatca tctgtggtca ttagaagcag tatactttaa aggaggggaa
    20221 aaagtagagg tatatgatgc tgattttggt agatttggtg taatgatatg ttatgatgca
    20281 ggttttccag aagtatccag agaattgaca ttaaaaggtt ctgaaataat atttatacca
    20341 tctgcatgga gaatacaaga tgaagatatg tgggatttaa atgtatctca aagagcttta
    20401 gagaacactg tatatacagt aggtgtaaat ttagtaagta atgactctaa tttaatacta
    20461 tttggaaaga gtaaaatatg caatcctaga ggtactgtaa taactcaatt agatacatac
    20521 agggaaaaat atgtattatc agagattgat ttagatgaaa taaaaaagta tagggaagat
    20581 ataccctatt tgaaagacag aaaaagtagc tataacatat aattctatac tataatacaa
    20641 gttcaatata aaatgataaa aagaggatgt atcaatatta agattaaatt tattatattt
    20701 tgatatatcc tctttaatat tatcaacttt gatttaaata tttttaaaat agttgataag
    20761 aaagtttttg aacaacaaca cagctttaga ttcatagttt tcgctaaacc atgaaagatt
    20821 taaatatctt ttaaattcaa tatctttgat atgtaacaac ttaatctcag ggtcttgaga
    20881 taatccccat gattttccac atataaatcc aactccttga agtgatttta taagagcata
    20941 aattgtatat ggtgaatcag actcaaatgc tattttgggt ttgaaattag ctgattcgca
    21001 caggatatca attacctctc tataattctc acccttcgtt ataactataa agttttcatc
    21061 tgaaacttcg cttagatata cttcatcctt taatgataaa ggatggttta ttgaaactcc
    21121 taatagtatt tcttcgcatg ttaaagttat gctattttct gagtttagct ttgtaaatga
    21181 agaagatatg tacaaatcaa aatcactttt tttataagat gatggcaaag tatgtgacac
    21241 attaaaagta atattgggat atagttttct gaaattactc aaaagtgatg gtaacacatt
    21301 agccgctgca ggagataata tctttacttc tcctgaattt tctaaagaag tgtcttgtaa
    21361 ctcctttttt gaactttcta ctaaatctaa aatgctatct actcttttta gaaacaatct
    21421 tccatactca tttaaaacaa tatatcttcc ttttctgtca aataactgaa ctccaagttc
    21481 tttttctaag gaagatatag ttttgcttaa agctggctgt gctatattta acttgtgtga
    21541 tgcttttgtt atgtgctcca ttctagcaac agtttgaaaa tacttaagat gcaataaatc
    21601 catagtataa aatacacctc ttttaatatg acttgagtta tatcaatttt ataatcaact
    21661 ccacattata cattataata taaagtttat ataatcaact acaatatatt aaaaagttat
    21721 ttaaattgtt atttatattt tgtacataat ctaattaaaa aaagtagctt taaatgatta
    21781 actaaggaaa tgttgttaca aactttagtt atataaaaaa ttcatttgat gagtaaagta
    21841 ttaatgtcaa gtaagaataa aagcttatta gatagaaaaa taatatttta taataatgat
    21901 gttacttaaa ttattatgtt atactatttt aaataggaaa catttagata ctataatttt
    21961 agggggagtc aagatgaaat taactaaatc aatatcatca atgctatgct tagctctagt
    22021 tttaagctta tcaccagtag catatgcaaa tgaaataagt agaacaacag tggagaatac
    22081 tttagtaggt caggacagat accaaactgc tgttgaagta agcaaaaaag gatggagtag
    22141 tgcgaatgag gctgtagttg taaatagtga atctattgta gatgcattag ctgtaacacc
    22201 atttgctaaa ttaaaaaatg ctcctatatt attgacagaa aaaaataatc taaattcaca
    22261 aactgagaaa gaattaaaaa gattaggagt taaaaaagtt tatataattg gtctatctag
    22321 ttctgtatca aatgatgtgc aaaaacagtt agaatctaat aatttaacta ctgatagagt
    22381 tggtggtagt gatagatacc aaacagcttt gagtatagct aaaaaaatag aaggattaaa
    22441 agatatatca gaaatagctg tagtaaatgg gtacacaggt cttgcagatg cagttagtat
    22501 agcttctgta gctgctacta atggaatggt tatacttcca gtatctgatg catctggtat
    22561 atctagcttt aaagatttta tagaatctaa gaatataaat aaatcataca taataggctc
    22621 tactaatgct gtatcagata aaataaaaca aagtttacct aattcagaaa gaataggtgg
    22681 agcagataga aatgaaacta atggtaaagt aatagaaaag ttttatacat ctaataaatt
    22741 aaacaatgta tttgtggcta aggatggaat gaaaaatcaa aatcaactta tagacgctct
    22801 agcagtagga gtactggcat caaaagaaaa ttctcctgta ctaatcgtag gagaacaatt
    22861 aagtgcaaca caaaagagtg tatttttgaa taaaaatgct acctctataa ctcaagttgg
    22921 tagtggagga aatgaaaatg catttaaaga attaaaagat ttacaggcaa atagtaacaa
    22981 taagagagta atgtatgtaa ctaatacaga taaggtaaat ataagaaatg atgctacaat
    23041 agaagcttct gttataggat atctaaataa tggtgatgaa gtagaagttt tagatgtgtt
    23101 aaaaactgga tgggtaaaaa taaaatacaa tgaaggtatt ggatatgtta gtgggtctta
    23161 tttgacaaat aataagcctg ataatagtaa tgaaaatata aaaataaagt atgtaaaaga
    23221 aaaagatggt ctaaatgtta ggaaaggacc aagtacagaa gatgaaaaaa taggtcacct
    23281 ttcatatgga agcaaagtag aaacaataga aatatttgct actggatggg taaaaataaa
    23341 gtataatggt ggatatggtt atgtaagtaa tgattactta tctgatacac cagttattta
    23401 attataagta acaataaaaa actgctttaa tttgtaaagc agttttttta taaaaattta
    23461 agtctaataa ctatttctat tttgatacct taatcctggg atttattatt atggatatta
    23521 tcaacataca aacactccaa atccagtgtc taattccaaa cttagatatt aataaattca
    23581 gtcctgctat ggtaaataag cagtatttaa ttacaattaa aagtgtattt atagaaatcc
    23641 ccttcataga aagtatccta aatccaataa tagacattct aagtcctata aatgccaata
    23701 atatacctgc aatatcctga attaagtaat agtattcaaa tttcatattc aatcaactct
    23761 tctctgagca gtatcaaata tttctaactc ttcaatttca tcatctgtgt atgtaagcat
    23821 agtaaaacca gttacaccat aaataaatga aattataggt gtaatccaac ttaagaaaca
    23881 atatggaata aattctagtg ggcttacacc aagagtctga gcacagaaaa cagcatttga
    23941 attccatggt attaatggac caccaagtgt agctgtatct tctataattc tagatagatt
    24001 ttgtggtttt aatttaaact ctttgtatat tggagacatt agagttccga caaatacaga
    24061 agtaaacatc attgatgaac taaatgcatt tgcaaagtag cttactataa aagtagaccc
    24121 aaccaattta gttcttgatt ttcctatttt agttataaga ggttgaagca gtacttctaa
    24181 gaatccagtt ttttggagaa taccggcaat aacgaaaact acaaatacta gtaaaacggt
    24241 ttcagccata ctcattattc caccacgatt taatagtttg tcggcataga caaatccagt
    24301 atctatagta aatcctgata gcatgtaatt taaaatatca cctatttttt ctccttcttg
    24361 aaatactgct ataataagtc ccatgatagc tgaacttagt atagatataa tgggtggctt
    24421 ttgtagcaat aaaagtaata ataaaaatat tattggaatc attgctacta aaccaatatg
    24481 aaagtttgaa tttaatacat ccttgatttg atttatttga acatagtcta tagtattatt
    24541 tgaatactta aatccaatta ctgtatacag aactatacat attacgtatg caggtcctgt
    24601 ggtataaaac atatgcttca tatgtgtaat tacatctgtc ttacaaactg ctgcagctaa
    24661 gtttgtagaa tctgatagag gtgacatctt atctccaaag aaagcaccag aaatgactgc
    24721 accagctgtt aaaccaagag gaatacccat actagtacca atactcatca ttgctatacc
    24781 agaagttcca agagtccccc aagatgtacc agtagcaaca gaagttaatg aacatacgat
    24841 taatgttgta agcagaaaat atttaggggt tattattgtt agtccactat atactactgt
    24901 tggtattgta ccagcagcta tccaagctgc tattagtaca ccaacagaaa gtataattaa
    24961 gtttgattga aaggcatctt taccaacttc aaatccaaaa gcttctattt catcattagt
    25021 ataaccaagt ttcgtggctg caggaactac tataagccat gaaagtaaaa acattgtagt
    25081 tatagaagca ttaaatacaa ctataccaac tgatgttaga gctattattg caagcattac
    25141 taacaaggaa tatctaaatg aaggtatttt tctttctgta ttcattttca tagtgcaaat
    25201 tctccttaaa tatttttatt taattgcatt ttttattctt ataagagctt cttcaagcat
    25261 acttcttgga caagctaagt ttattctttg atacccactt cctccaatgc caaagctgtt
    25321 tccttgattt agagcaactt tacctttttg tacaagtaca ctttctagtt cctcatcact
    25381 taatccaagt gcactaaagt ctacccataa taaataagta ccttctggtt ttctaacttt
    25441 aagttttggc atattttcat ttatatattt tatagcaaaa tctatattag attctaaata
    25501 ttctaaaaat gattctagcc aagattcacc attattatat gaagcttcag ttgcaactaa
    25561 gctaaagcaa ttatttctct ttatatcaat tctggtaaaa gcatcatcta acaatttata
    25621 gtctttttca tcaggtagta ttacatatga tgattgaaga ccagctatat taaaagtttt
    25681 agttggagcc atacaagtaa ttgtattttt ttcaaattct ttagaaatag atgccatagg
    25741 aatatgctta tgttttttta atataatgtc actatgtatt tcatcagata tgatttttac
    25801 attatgctta agacaaatat ctcctagttt ttttaattca tcttttgtcc atactctacc
    25861 aactggatta tgaggattac aaagtatgaa taattttaca tctttaatct tgttttcaat
    25921 gtcttcataa tccattatat agtttccatt ttctagtttc tgtaatgggc ttataattag
    25981 ttctctattg ttatttttta caacactatt aaaagggctg taaactggtt cttgtatcat
    26041 tattttatca tttgcttttg tgagttcatt tattaaaaga cttatagctg ggataacacc
    26101 tggactataa attaaccact cactttttat tttccagtta tgtctcctat ctagccagtt
    26161 tactatagat tcattgtaag aatctggtct tgttgtgtag ccataaatct cttgttctag
    26221 tctatttttt aaggaatcaa ttatacaagg tgcagcctta aaatccatgt cagccaccca
    26281 cataggaagt aaatcattag ttccatactt tttttccatt tctgaccatt tagatgaaaa
    26341 attattgctt ctatcaacaa tctcattaaa attataattc attgtaaata cctccagtaa
    26401 ttttaatttt atgttaataa tattaagcaa acaacgtgcc aatattgata ataaaaaata
    26461 tttattcaca agttaatatt taatattaag taatttcaga gcatagataa gataaaaata
    26521 ttaattatac ttaaaacttt gtggaaaagg ttatttctaa taaattttta aatatatgaa
    26581 aatgtaatta aataggttaa ataggtttaa ctgtttaact tgttagacct atttatattt
    26641 tttagaaatt ctactccttt tgatgttata attgaacctc cacgaccaac aagtatgtct
    26701 acaagcccaa agtcttgtag ttctaaaagc atatttctaa tctgttgttc tgtcaaaaat
    26761 ctattttcct ctaaagctat tttacataag ctttttctac ctattctttc ttttaaatca
    26821 taagcctttt ttaaattatc caatataaat atgtattcat ctagtgacct tttaaaatta
    26881 tagtcgtatt tcatgatgtt tttgtctttg ccaatgttga attgatactt ttttatatta
    26941 tcacttactt tattacaaac ggttctacta taatttgaat ctatagtgtc caatatatat
    27001 tcaggtaaat cacatatttc tataatatct tttcccaagt aacagaagta ttctcctaag
    27061 tttcttaatt ctcttacatt tccttcccat ctatacattt taaagatttc ttttacttcc
    27121 tcacttagaa taaaattaca tggaatatca tattttaatg aaccaaaaat ttcaaatata
    27181 tcagcagctc tttccctaag tggatgtatt ttaaggggaa gaacattcaa cctaaaatat
    27241 aaatctcttc taaacatatt tttagataca agttctttta aatttctatt agtagctgca
    27301 attattctta catcaatatc aatgacatta ttagaaccaa tacgtcgtac ttgtttttct
    27361 tgtatgactc taagtaattt agattgagaa tttaaatcca tttctccaat ttcgtctaaa
    27421 aatatagttc cattgttggc taattcaaat aaaccaattt tcccaccttt tttagcacca
    27481 gtaaaagccc cttcatcata gccaaataat tcactttgca gtagattttc ttgaaaagta
    27541 ctacaattta ctgcgacaaa gggaccatct tttctcctag aagcactatg aatggattga
    27601 gcaaataatt ctttaccagt accactttca ccaattataa ggatagatga atttgattga
    27661 gccattttat tagcaatttt ttttgtatta atagtttgta tactagaacc taatatatca
    27721 tcaaatgtgt atttactgat attaccagag tttactagtt gtgctcttaa tttagcttgc
    27781 tttttttcta attgagaaaa ctttgtcatc tttagtataa atccatcaaa tacatttaat
    27841 tgtatatgtt taatctctaa atttatatca atatgattta ttttaatcaa tttctcaaaa
    27901 aatggagttt tattggtcaa tatgttttga aaattgatgt cttttataca atcaccaaca
    27961 aatgagtcaa tcatttcatt tgcatttata gacaaaattt ttctagctat atgattatag
    28021 aattgaatta ttccatcatt atttgtgcat ataattccat catcaattat agatagtaac
    28081 aaatcaaatt gtctttcaaa cttatttgca tctaacaata actcacctgt actataactt
    28141 gtaggaatta ttttttctac gtattttttt attaggtcat cttttattag atgttttaat
    28201 ttggtttttg tagcaatttc aacaatacaa cttaaatcta ttattctgta ttttaaatct
    28261 acaacttcac aatttttaaa cttaggcagg attttttcgc caggagttag gattacagca
    28321 tctgtgggag taaactcaat ctctggatag cagggcagga tatttatatt gtctattcct
    28381 aatctatgta ttaaagaaat tgtttctatt gccatatctt tacttaagtt atatacataa
    28441 ataattttat ctttttctaa tttggatatc atatcaatac tgttcttttc aaatgtcaac
    28501 ttgggaacaa tgatttgagc atcatagtga gataatttta ctatttcatc gtattttaaa
    28561 taagcagata ggagtataag tttttcttta aaaaacttta aatttccctc ttcaaatgag
    28621 tataaattta tctttagatt atcacctaag aattgcctta tttgattttc ataaatttct
    28681 ccagcgtctt tttttaaact tacaatagct aattccattt gtttcatttt aacctctcat
    28741 acaaatttga cttattttag attacataat aattaaatta gattaaataa tattatttaa
    28801 ttattattat aaatttatta tagactacac attgaagtaa aagaagtagc taaaattatt
    28861 ccttttattt ttgttttttg tatctatttg gtataatagt attagagcgt ataataattt
    28921 aggcaaaatt ttttaaggaa gactagagga gaatataaaa tgaaaaaatg tctttactgt
    28981 aatagggata taaagtcacc aaataataaa ttagctataa aatatgatga caatatgaat
    29041 atatatccat gtcgtgcaga atgtaaggaa aaaattgaag aatatatagc aaaaaaacct
    29101 acatataagt ttataaatat attagaggtt cttttaggtg tgggcggaat attctgcttt
    29161 ttgagtaaat ggactattgc agcccaagtt gtacttggaa ttgatgcact ggttatattt
    29221 ttattcccat tggcaggatt taagatgaat ggaaagttaa gtatggaaca aactaagaat
    29281 caaagtaggt ctattgcaat atcaattttg gcatttatag taataataac cgtattatat
    29341 tttaaacaag ttggtaagta gagcataatt gtataagaca tcattagagt atgaggtgag
    29401 tttatggaag aaagaaaatt agcaccaaac aatgagaggg aagctctcat aaaaagatta
    29461 aaaagaatag aaggtcaagt taaggggata caaaagatgg tggaacaaga acgttattgt
    29521 gttgatatat tggttcaaat atctgctata aggtctgcta taaatagagt aggcacaatt
    29581 atcttagaga atcatataaa aggatgtgta gcagaaagca ttaaacatga tgatgctgat
    29641 aaaactgaag agatgataaa agagcttatg gatactataa ataaatttac aaaataagga
    29701 ggcggttagt tatgaatcaa caaggattta ttatggaaat tgttgatgat agaactgcaa
    29761 agttaaaaat gcaaagacat tcagcttgtg ctgcttgtgg aaaatgtgct actacaaatt
    29821 cagcagagtc aaaagaaata ttagtagaag tagacaacac aattggtgca aaagttggtg
    29881 accatgtaga agtgagtatg gataatatga atgtattaaa agcagctgta atggcttata
    29941 taatacctct tatagcactt cttgtaggaa caatagggac ttattatata ttgggggcaa
    30001 ttggaatgac aactggagtt gaagccataa gtggattagt tggaatttgt cttacaataa
    30061 tctgttattt atacttaaag aagaatgaca aaaaatttag agacagtaga gaatttatac
    30121 cagtaataac aagaatatta attgaccttt aagataatta taattaaaaa atagcaatgt
    30181 gctttttaaa taaatactca atacgtattt gtagagttta agtacattgc tatttttata
    30241 tttttatttt gattttctgt aaggaataaa gttttttctg attttcaaaa gattcaatgt
    30301 tcttacaaaa gaagttacaa ctaatacacc aagtgctatt gcagcactta gaaatagcgc
    30361 atctgtacct tttgacatag ccaaagaata atttttctga atcaaagaca acattgtatt
    30421 atatagccca attcctggta ttagaggaag aatacctgga attacgaaca caatagctgg
    30481 ttgctttagt tttcttgcta aagtctcact acacgctgag accaaagcag cagcaataaa
    30541 acctgcaaaa actgcatttg tagtaaaatt aaataaataa acatatacaa tccaaccaat
    30601 tccgccagtt actccagctg gaagaagtag atatagtgga gcattaaaga aaacagcaaa
    30661 tccagcagtt gcaacacctg aaaataaaaa atgcaaaatc atagacatat cagtcattag
    30721 aaaggacctc ctattctata ccatgtatca aggacaaaac caactccaga agcaattgca
    30781 ataataatca tagcagcatc aaaagcacga gaaattcctg atattaaatt ccctgatatt
    30841 aaatctctaa tagcttttat aaaagcaact cctgggagta gtggcattat agcgccgact
    30901 ataagcattt tagggtcatc taaaatgcct atttcaacta gtagaactcc tgaaagtgca
    30961 attaaaaaag aagatactaa acaggaaaat gcagatattg cacttatctt catcgtttta
    31021 tcataagtaa ttgcagcaaa gattgctatt ataaacgtaa gtataaaatt taatacataa
    31081 ttccctccaa gcaaacaagc aaaacaagca gatgcaattc ctgtacaaga gtaaaataac
    31141 cataaaggat aaggttttac atcttggatt tcataaagac gtgcaatggc attatcaata
    31201 tcatactctt tgtcactaac aaattctcga gaaaaactgt ttaataaaga aatcttgttt
    31261 aagtttatac ctcttgattg aatcgttttc ataaacgaaa agccatcaaa tttctcatct
    31321 gaaataatta ttactgtagg agttgtaaaa atatttacat gataaaaccc tcttgacctg
    31381 catattctaa ggacactgtc ttcaacccta gatgtttcag ctccattttt tatcatcagt
    31441 tcccctacaa atagagctag ttttagaata tttttcttat attcaagttc ctcattttga
    31501 ttcataaata cacctccaaa ttaaaaaacg taatattgaa attatatcaa atttttaagt
    31561 aatattcctt aatatct
//
LOCUS       DAMJWP010000019        30577 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.020, whole genome shotgun sequence.
ACCESSION   DAMJWP010000019 DAMJWP010000000
VERSION     DAMJWP010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30577
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.020"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(48..209)
                     /locus_tag="PC700_000807"
     CDS             complement(48..209)
                     /locus_tag="PC700_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580131.1"
                     /translation="MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFD
                     FKIKFHKFK"
     gene            complement(550..990)
                     /locus_tag="PC700_000808"
     CDS             complement(550..990)
                     /locus_tag="PC700_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2580132.1"
                     /translation="MSNLSAFLSQNAIKVDNVKYVASNRFLDKEGKPVEWELKVLSSE
                     EDEALRRKCTKRVKVIGNNGKHTGQYTSEIDYNSYVAELCVASTVFPDLKDAELQNSY
                     GVMGEAQLLKTMLTAGEYVNYTVKVNEVNGFDTSFEDKVEEAKN"
     gene            complement(1062..1889)
                     /locus_tag="PC700_000809"
     CDS             complement(1062..1889)
                     /locus_tag="PC700_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580133.1"
                     /translation="MEFESGIKLGKLTLIKYLGRNKHSKKVWLCQCECGNKVERVENN
                     LKQSIKNEKPPHCGCSPNWKGQNKRFKDITGKTFGKLTVLKMSGKDKYSHNLWLCQCE
                     CGNRTITSTSALEQGKAQSCGCIRKEILLERSTTHNKSNDSLYRVYHRMIKRCTNKSN
                     KDYNYYGGRGIEVCNEWLEDFINFYNWSIENGYRKGLTIDRINVNGNYEPSNCRWVTW
                     EVQRNNKRNSIRVNYKGEMITLKQCAENLNVKYLFLYNQLVTKKIPLEEVIKNIGME"
     gene            complement(1948..2418)
                     /locus_tag="PC700_000810"
     CDS             complement(1948..2418)
                     /locus_tag="PC700_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HDF2580134.1"
                     /translation="MAQTINAKDTVSAKKAECFITIEGKRYNFMQAIDLEAKMEKNKS
                     EVPILGRTTKGNKTTGSTNTGSATFHYNTSIFRELLYRYKETGEDIYFDIQVTNEDPT
                     SAVGRQTVVLKDCNMDSGIITKFDADGEYLDEDMDFTFEDWELVESFALLNGML"
     gene            complement(2435..3745)
                     /locus_tag="PC700_000811"
     CDS             complement(2435..3745)
                     /locus_tag="PC700_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HDF2580135.1"
                     /translation="MALGGGTFVTQNKILPGAYINFISAKRATSSLSDRGIVAMPLEL
                     DWGIDEEAFQVTSDDFEKYSVKYFGYDYTHEKLKGLRDLFKNIRLGYFYKLNKGVKAS
                     CTIATAKYSGIRGNDLKVTVTTNIDDNAKFDVVILLDNKKVDTQIAKVITDLQDNDYI
                     TWKKDATLEASAGLVFTGGTNGEAVTGAEYQAFLDKIESYSFNALGCLATTTEIKSLF
                     VEFTKRMRDKVGAKFQTVLYKKSDADYEGVVSIENKIKDKDLVESSLIYWATGAIAGC
                     DINKSNTNKKYDGEFDVDVNYTQIQLEEALKTGKFIFHKVGDEVHVLEDINTFVSFTD
                     DKNDDFSSNQSVRVLDQIANDIATLFNEKYLGKVPNDKAGRISFWNDVVKHHKELENI
                     RAIEDFKTDDVSVELGNDKKTVIVSDAIKVINAMSKLYMTVSVN"
     gene            complement(3746..3922)
                     /locus_tag="PC700_000812"
     CDS             complement(3746..3922)
                     /locus_tag="PC700_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580136.1"
                     /translation="MSKALSKEDNYKFTKEQIVNSKKYVNRKDLLNAILKENELYSFS
                     EVEDRINKFMKGVS"
     gene            complement(3915..4352)
                     /locus_tag="PC700_000813"
     CDS             complement(3915..4352)
                     /locus_tag="PC700_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580137.1"
                     /translation="MLNNIIDGISVKLDKSFGEKYTIYSEDVEQGINEPCFFIVPLNP
                     SKTPYPSGRELKKNSFDVHYFPRSEDKNFEINEIAEMLLEELEYIEIDGDLVRGTNMN
                     FEIIDNVLHFFVDYNYFTIKNNDTNKMDTVELFGGLKRGDNFE"
     gene            complement(4701..5507)
                     /locus_tag="PC700_000814"
     CDS             complement(4701..5507)
                     /locus_tag="PC700_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580138.1"
                     /translation="MAKFLDITGKKFGRLRVIKFSKEIKSGKRNRKYWLCKCDCGNFK
                     EIRTDSLTSGLVQSCGCLKKEQDKLNLTDKYQFKKKYKVQNKRLYSIWKGIISRCTDK
                     NNKRYNRYGERNIIVCDEWFCYDNFANWALSNGYSEKLTIDRINNEGNYESSNCRWVD
                     IKTQCRNRSTNILVKHEEKEITLIELSEKTGISYSCLRSRYSKGLVGNKLIEKVKIIE
                     ESRAKLSIEDVKEIRKKYSDGYTIKQLSEIYPVTYSSISNIVHRRTWKNI"
     gene            complement(5617..5907)
                     /locus_tag="PC700_000815"
     CDS             complement(5617..5907)
                     /locus_tag="PC700_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDF2580139.1"
                     /translation="MARWGSVDFREFKRVCKKMEKFTKIDLDKFCKDAARELAARLLG
                     KVIRRTPVDTGFLREGWSGVAYARSLPVYKQGNNYIIEVVNPTEYALTQWGI"
     gene            complement(5907..6254)
                     /locus_tag="PC700_000816"
     CDS             complement(5907..6254)
                     /locus_tag="PC700_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580140.1"
                     /translation="MVSKTRKAIEMLYRDKCTIVEYQPIKDPVTKRTNNKEVIVLENQ
                     PCKLSYKNIVSATEGKLAKLEQTIKLFISPDIEIKAGSKLIINDKEYVRSGESAIYPN
                     HQEIILELFKDKA"
     gene            complement(6248..6649)
                     /locus_tag="PC700_000817"
     CDS             complement(6248..6649)
                     /locus_tag="PC700_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580141.1"
                     /translation="MENNIIDEIEKRLESFGYILKDGDKWLIGFVREKIENIIKLDCN
                     IKTMPIELKEIEVDMIVGEFLFTKKNMGQLDIESINFEAVEKSISEGDTKVDFAIGSG
                     SQTPEQRFDSLIAYLTTYGKNKILTFRCLRW"
     gene            complement(6659..6892)
                     /locus_tag="PC700_000818"
     CDS             complement(6659..6892)
                     /locus_tag="PC700_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580142.1"
                     /translation="MPQVKKLNRILTIEECKIDDFLEMGYDLIDETGKAVKYGKSLSV
                     KDLIAENNILRSKVESLEEENKQLKEKNKLTKK"
     gene            complement(6911..7801)
                     /locus_tag="PC700_000819"
     CDS             complement(6911..7801)
                     /locus_tag="PC700_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HDF2580143.1"
                     /translation="MAALNYAKEYSNVLAQAYPYTLNFGDLYATENNGRYRWTGSKTI
                     EIPTISTTGRVDSNRDTIAVAQRNYDNAWEPKVLTNQRKWSTLVHPADINQTNYVASI
                     GNITKVYNEEQKFPEMDAYCISKIYADWTALGNTADTTVLTTANVLEVFDKLMEKMTE
                     ARVPENGRILYVTPVVNTLIKNAKEIQRTVNIKDGGTSLNRQTTDIDTVKIIKVPSNL
                     MKTAYDFTTGWKVGAGAKQIFMSLVHPSAIITPVSYQFSKLDEPTAVTEGKYFYFEES
                     FEDVFILNKKADAIQFVVEA"
     gene            complement(7813..8376)
                     /locus_tag="PC700_000820"
     CDS             complement(7813..8376)
                     /locus_tag="PC700_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HDF2580144.1"
                     /translation="MDWLKELLEGIKVEDNKIDVASLQKSIEKKIKETTVTQEDYVNL
                     ETQLNTANETIKKFEGGMTKEDVENLKTTYETDKKTLEETYKKEIEEKDFNYWLGDAF
                     KSVKCRDEIALKAHLDMEALRNSKDRQKAFEEQINPLKQDKDYLFNATLEGEEPKIDT
                     ITPGQEPKINDFGFNFTGVRPHENNNK"
     gene            complement(8444..8692)
                     /locus_tag="PC700_000821"
     CDS             complement(8444..8692)
                     /locus_tag="PC700_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580145.1"
                     /translation="MDNKFKNLQHLIDSIDLGLDIEFDLYNKPYNISIGDDDTRFITL
                     CPNGDTKYYKNGKDMVDNYNIDGKLLKDLWKDIQIVNM"
     gene            complement(8694..10229)
                     /locus_tag="PC700_000822"
     CDS             complement(8694..10229)
                     /locus_tag="PC700_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HDF2580146.1"
                     /translation="MKHKDYWRKRFEQLEEVQNNKSVKYYLELEKKYKLAMNSIEKDI
                     LSWYNRFAENEGISLLEAKKLLNTRELEEFKWNVEEYIKYGKENAINQKWMKELENAS
                     ARLHITRLEALKLQIQQHVEVLYGNELDGIDKLMRDIYTSGYYHTAFNVQQGVNVGWS
                     LMSLDTNRINKIISKPWATDGLNFSERIWGKHRPTLINELHTKLTQSIIRGENPKNLV
                     NDFAKRFNVSKSQAKNLIMTESAFFASASRKDCFSDLDVEKYEIIATLDLRTSNICRE
                     LDGKIFDMKDYQVGITAPPFHCRCRTTTAPWFEDEEGYRAARGEDGKTYYVPSSMKYN
                     EWYEKHIEGRLGKEKADTLRKMQLNESKDRKQFEEYKKVLGNEIPSKFDEYQHMKYND
                     TIRYEEAKKLYKDVNWQVKNQRNLTSGSVHSVPFESKPNSVFDNYKDGKLIQRRYYGN
                     TGKPRLDLDLTDHRNPKQHKIVPHKHDWLADENNHDKVKREKDMELTKAHKIANKDIL
                     NGE"
     gene            complement(10219..11661)
                     /locus_tag="PC700_000823"
     CDS             complement(10219..11661)
                     /locus_tag="PC700_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2580147.1"
                     /translation="MLNIYISETDLIKVQLKKESTFNLVKVIEHYILKHRPEKYKQGE
                     EYYYGNTDVNNKRRYYLLDGAKVDDFTKVNNKAINNYHKLLVDQKVGYSVGNPIVFNA
                     DDDNLTKLLNDLLGEEFDDTITELYLNASNKGVEWLHPYINRKGEFKYVIIPAEEAIP
                     IWDSKRQRELVAFIRFYYIEDIDGNKIKRVEYYTENDVTYFIERGNSFIQEFLYDEYG
                     KMTDIQEGHFRINNKEQGWGKVPFIPFKNNEKCVSDLTFYKSLIDIYDNNISTLADNL
                     DEMQEVIYVLKEYPGTSLQEFIDNIRYYKSIKVDGGGGVDKLEINIPVEAKKELLDRL
                     EKNIIIFGQGVNPESQNTGDKSGVALKFLYSLLDLKCSKTEKKFKKAIRELLWFVCEY
                     LKISGSKSYDYKSVQITFNHSMIINESEKIDMASKSIGIISDETIVSNHPWVEDVNDE
                     LERLKKQEETQKEYDDLIPTNNQEGVIDET"
     gene            complement(11667..12920)
                     /locus_tag="PC700_000824"
     CDS             complement(11667..12920)
                     /locus_tag="PC700_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HDF2580148.1"
                     /translation="MVIEMIVRINFNPDFKEANFTKKRYRAMKGSAGSGKSVNVAQDY
                     ILKLGDKKYQGANLLVVRKSEATHKYSTYAELTGAINRIYGKQADKYWKTTLNPLEIK
                     SKVTGNSIIFRGVNDAKQREKLKSINFSKGKLTWVWCEEATELMESDIDILDDRLRGI
                     LTNPNLYYQMTFTFNPVSATHWIKRKYFDYKNDDIFTHHSTYLQNRFIDEAYYRRMQM
                     RKEQDPEGYKVYGLGEWGETGGAILKNYVIHEFPTEFEYFDNMRLSQDFGFNHANAIL
                     RIGFKDGELYICNEIYVHEMDTSEIIKIANSRGLEKTLFMYCDSAEPDRIKMWKSAGY
                     KAKGVKKGPGSVKAQIDYLKQLRIHVHPSCTNTIKEIQQWKWKQDERTGLYLDEPVEF
                     MDDAMAALRYSIDNKLKNNGISFLK"
     gene            complement(12898..13590)
                     /locus_tag="PC700_000825"
     CDS             complement(12898..13590)
                     /locus_tag="PC700_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2580149.1"
                     /translation="MQDVKEKVKQDYLKGMKQKEISAKYDISLNTLKSWIKRYNWSSE
                     KKKGAPINKRGAPFSNKNSVGYGAPKENKNAEKFGFFSKYLPEETRELIQEISIKDKF
                     DILWEQITIQYAAIIRAQKIMYVKDKEEMIKELKKHEITENSEKIEYEFQFAWDRQAS
                     FLNAQSRAMSELRSLIKQYDEMIHKDWNLATEEQKTRVEKLKCEVANLNKDDIGDDEL
                     KISVDYGDRNDS"
     gene            complement(13660..14169)
                     /locus_tag="PC700_000826"
     CDS             complement(13660..14169)
                     /locus_tag="PC700_000826"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580150.1"
                     /translation="MYKKVLNILLNYYKAINSKEVVELVLTPVLICIISYMFFNSSIN
                     KEVVISLNKDVLTLSGLLVAFGVCIITLLFTTYNKSISEAKDIKTERKVNGFNISYFQ
                     FIQLKAYYTVIMEILVVVICFINTIMLTRYYSNIIFYVLIFFTVHIILSLTTLIISMF
                     HLSWKDRGD"
     gene            complement(14190..15164)
                     /locus_tag="PC700_000827"
     CDS             complement(14190..15164)
                     /locus_tag="PC700_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580151.1"
                     /translation="MSNFKKRYIDVYRIDIINKINGSVYSDEEILKEVIKTFFKDKEY
                     CKENKNGINITKDKKFWITEVKCSEDDLIIKVKLEYTKYNQNTNIINAHTKEVEGNKK
                     MDQGDSNKQHLFIKFMKENNIAVVLFERVFVGVPMIELSKNLHLYYEDMIENNKFDNN
                     MSKTSINITQIASKDFIEQILDLDGVSKITLNVDREKFGTDEDNLFSNLNNSRRHNDL
                     IYKPTFRTRYLPSEVKRCCEKYIKGEEFRNKKINRIIIEGEKNKRKLKLDTEGIKLCR
                     EIEVSLDIDNHIDSEDIFEKMNNLIDDLIENNSVFFNLMYQEVAMSEA"
     gene            complement(15359..15908)
                     /locus_tag="PC700_000828"
                     /pseudo
     CDS             complement(15359..15908)
                     /locus_tag="PC700_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994390.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
     gene            complement(16191..16670)
                     /locus_tag="PC700_000829"
     CDS             complement(16191..16670)
                     /locus_tag="PC700_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 region 4 domain-containing protein"
                     /protein_id="HDF2580152.1"
                     /translation="MDKESIKEIVRELRKEEREDRKKGVLYNTRLLMKHYNDFKRHID
                     SAVSEAKDVDYLEDDLGKLDNEELYILSIKKSKARTIIMIAHIDSAMKTLKLRQERLH
                     SYEKYRALELYYCDEKTYEEIAEILNCGVVTSRRWINEMVKELGVYLFGIDGLKLTV"
     gene            complement(16663..16800)
                     /locus_tag="PC700_000830"
     CDS             complement(16663..16800)
                     /locus_tag="PC700_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580153.1"
                     /translation="MELISPGKIVRCAWCGRRFYKLDKSKVEYCSRSCSKRARRSDLS
                     G"
     gene            complement(16824..18194)
                     /locus_tag="PC700_000831"
     CDS             complement(16824..18194)
                     /locus_tag="PC700_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2580154.1"
                     /translation="MEFKPHPYQEYTIRKTIDNNNIGLLLDMGLGKTVCTLTAISELM
                     YDYFDISKVLVIAPLRVARDTWSSEVKKWEHLKHLKVSKVLGSKLDRVRALSTDSDIY
                     IINREMVPWIVDFYKRKWPFDMVVIDELSSFKSNKAQRFKSLKKVLPLTKRVVGLTGT
                     PTPNSLIDLWAQMYLLDRGERLGKTITGYKERYFEPGQKNYQTGAIYNWQPKNGAENA
                     IHNKIKDICISLKAEDYLNMPRKIDNKIEIHLDSKILKYYKELEKEKILELDRDIITA
                     SSAAVAANKLLQLANGAIYDNDKNVKELHREKLEALKEIIDVSNGKPIIVFYNYKHDY
                     NRLMKEFKSLKPRTIENSKDIYDWNNGRIQLLLCHPASTGHGLNLQSGGSIIVWFGLT
                     WSLELYQQANARLYRQGQRETVIIHHLICKGTIDEQVMEAIENKDKGQSALLEAVKAK
                     LKEYRE"
     gene            complement(18143..18469)
                     /locus_tag="PC700_000832"
     CDS             complement(18143..18469)
                     /locus_tag="PC700_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HDF2580155.1"
                     /translation="MLESKIEKRLKKEIELLGGKAMKFISPGEAGVPDRIVLLPEGHV
                     IFVELKAPGKKLRKLQQYKMRELRELGFKVKCVSTLKEIDDFIKEVKGWNLNHIHTKN
                     TQLEKL"
     gene            complement(18751..21168)
                     /locus_tag="PC700_000833"
     CDS             complement(18751..21168)
                     /locus_tag="PC700_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580156.1"
                     /translation="MDIKASEIEHINVRHDGQLMLAIGKNKLETHWKNKSILWSELVN
                     RLSKTLRTQETYTEYRKMSKTEKDRVKDVGGFVGGSLKNGRRKAENIANRSIITLDID
                     YANKDIWEDITLLNDYACLMYSTHSHTENNPRYRLVIPLTRPVLPEEYQAISRMIADT
                     IGIDMFDDTTYQPHRLMYFPSTSIDGDYIFKFQDGEFLNPNEILDLYLDWTDVSYWPE
                     SSRERQKFNTQLKKQQDPIEKAGIIGAFCRSYSIKETIENFLNEVYIPGIDETRYTYS
                     EGSTSGGVVIYDDKFSYSHHGTDPASGILCNAFDLVRIHKFGELDEDAKPETPVNRLP
                     SFTRMSEFASSDTKVRKTIGRENLDKAKDDFGDIDFEDDEWLTRLDYDNKGSYKKTTN
                     NILMFIENDPYLKGKIAYNEFSNRAVVLGKLPWRKDDKLNDWNDSDDSGLRHHIETIY
                     NISSPSKVNDALIIAFENNTFHPIKDYLNSLKWDGIKRVDTLLIDYLGAEDNHYTRTI
                     IRKVLVAAVARVFNPGIKFDNMMVLSGPQGMGKSTFIKKLGGDWYSDSLTTVQGKEAY
                     EQLQGVWLLEMGEMMATKKADIEAVKHFLSKSEDIYRVAYGKRTSRFLRQCVVIGTTN
                     DKEFLRDKTGNRRFWPIDTGVKKVNKSIFNGQLDNERNQIWAEAVELCKANEQLYLSD
                     EEKKEAERQQRTHSEENAKSGIIEEYLNKPITENWYDLSISEKREYIHGSDFGDLKEG
                     TILREKTCVMEIWVELFNGEPKQLTPILSREINDILKGLDDWISYNGNLRFGKIYGRQ
                     RAYVRKS"
     gene            complement(21180..23138)
                     /locus_tag="PC700_000834"
     CDS             complement(21180..23138)
                     /locus_tag="PC700_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HDF2580157.1"
                     /translation="MRTLSIDIETYSDLDIKKVGVYRYVDSANFEILLFAYAFDNEEV
                     KVIDLVNDEELPKEVIEALNDNKVIKSAFNANFERTAISKFLNINLKPNEWSCTMIKA
                     LTLGLPGSLDSVSKALKFNEDKQKMKEGKALIQYFCKPCKATKVNKGRTRNLPIHDME
                     KWNKFKEYCKQDVVVEREIRNKLSKYKTTEREIKLWYLDQRINDTGIKVDTELIENAI
                     ECDKRYTEKLTKEAIKITGLNNPNSPAQLKKWLSDKVGFEITSLTKESIPEILKQVDD
                     ENVVRILELRKLMSKTSIKKYEAMKLAKGNDNRVRGLLQFYGANRTGRWAGRLVQVQN
                     LPQNHIEDLDLARNLLKEGDFDLIELLYDSVPDVLSQLIRTAFIPSEGHRFIVSDFSA
                     IEARVIAWFAGEKWRLDVFNSHGKIYEASASHMFKVPIESIKKGDPLRQKGKISELAL
                     GYGGSIGALTSMGAIKMGLDEEELQPLVTTWRNANPNITKFWWDVDKAAKKAIKDRTI
                     VKLQHGIKFIYNPGVLFIELPSGRRLSYLRPKIEPHTTFSGDKITYEGMEQTSKQWKR
                     IDTYGPKLVENIVQATARDCLREAMFNVTDAGYSIVMHVHDELVIDVDKKGGSLEEVN
                     SIMGKEISWAKGLPLKADGYECDYYKKD"
     gene            complement(23138..23710)
                     /locus_tag="PC700_000835"
     CDS             complement(23138..23710)
                     /locus_tag="PC700_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HDF2580158.1"
                     /translation="MSNSVQSTKVVTGKVRLSYCNIFKSRAMVEGAEPKYSVCILIPK
                     SDKVTLGRIKKAIDAAKEQGKTSKWGGKLPGNLKTPLRDGDAERADEAEEYVGMYFLN
                     ANSTQKPGIVDKDLNEILDNTEVYSGCYGRVSINFFPYNSAGNKGIGCGLQNVQKLAD
                     GEVLGGARASAEADFSDDFEYEDEEEDFLS"
     gene            complement(23733..24305)
                     /locus_tag="PC700_000836"
     CDS             complement(23733..24305)
                     /locus_tag="PC700_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HDF2580159.1"
                     /translation="MSNQEITKLVENNLNLVHFSINRFFKSYVEQHPYLYEEFYQEGC
                     IGLYKAALNYDSNRGKFSTVAVSYIRIEMFKALRYGEKHYRNSIEGLDVSVFEDSKRP
                     LKDIHGFYEIQFNYDVDYIRKIARKSYLKDIDKIVDWILQDKKISDIGELLNVSSTVI
                     CGRIKLFSKQVRDLDKFGEITSNIKSIYQI"
     gene            complement(24358..24693)
                     /locus_tag="PC700_000837"
     CDS             complement(24358..24693)
                     /locus_tag="PC700_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580160.1"
                     /translation="MPLDAGIKDKLDESISIILDLKDRKKSIEKDLDYEKDNLLDMLK
                     RLNITEYNSTEENAKVIVMDFKRESLIKDKVMSTFLEVNDNHLDRVYIPEHIKVSPVC
                     FVSVRAKDN"
     gene            complement(24708..25874)
                     /locus_tag="PC700_000838"
     CDS             complement(24708..25874)
                     /locus_tag="PC700_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HDF2580161.1"
                     /translation="MPLQHARLSASGAHRWLHCTPSIKLEENYPPLTSIYAEEGTVAH
                     ELAEVKLMLEYEKISKKAYNARIKKIQKSEYYNSEMEDYIQSYVENVVELVNDSKAIC
                     DDVIVMLEERLDFSEWVPEGFGTGDVVVISDGILQVIDLKYGKGLEVSAIENPQLRLY
                     GLGAYNQFEMLYDIDLIKTTIIQPRLDNISSEEIEVTKLLTWADNVKKKAQMAFNGEG
                     EFVSGSHCGFCRAKNDCRKRAEDNLKLARKYDFADTFALNKYEIADILGFAKNIQDWL
                     KDVQSYALEQAEKHGVKYPGYKLVEGRSNRKYVDEQEVAKVLLNSDYDEEKIYKPRTL
                     KGISDMEKAIGKKSFAKLLSDLIIKPVGKATLVVESDKRSEINSIDSAKKDFEI"
     gene            complement(25886..26323)
                     /locus_tag="PC700_000839"
     CDS             complement(25886..26323)
                     /locus_tag="PC700_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580162.1"
                     /translation="MVEVNVNVKVKVEAPEFTNALLVVANALGGLNLGKAMQIEPINI
                     TDMKEEKKVEVKKAEKIKKVEVKEDVKEEIAEAKEEIEKNNENTTSEVKYTKEEVRTK
                     AAQVSKAGKKDKLKELFGEFGASKLSEVKEEDYSAFMNKLESL"
     gene            complement(26394..26543)
                     /locus_tag="PC700_000840"
     CDS             complement(26394..26543)
                     /locus_tag="PC700_000840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580163.1"
                     /translation="MKLLSLDTNNKNVVTLKKDGKVIADIVFKDIKTGKKISVGILNK
                     KVLVK"
     gene            complement(26524..26916)
                     /locus_tag="PC700_000841"
     CDS             complement(26524..26916)
                     /locus_tag="PC700_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2580164.1"
                     /translation="MDISESIAKQFSDSLKSLIEIEISKQETDRVKSQTIEQKVKVLE
                     PKDIVVLIKRGYPNYLITVEEARGILKLDTAFMRRLVSTGLIKSLVRGDGRKISRYEV
                     DDFIERNQGKNLDELLKAVEWGDEIAES"
     gene            complement(26947..27144)
                     /locus_tag="PC700_000842"
     CDS             complement(26947..27144)
                     /locus_tag="PC700_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2580165.1"
                     /translation="MLIGDNIKQMLRKRDIKPYKLAKELNIDVSGLYKLLRNKSSNPT
                     IDTLIKLADYLDITLDELVGR"
     gene            27220..27594
                     /locus_tag="PC700_000843"
     CDS             27220..27594
                     /locus_tag="PC700_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580166.1"
                     /translation="MQFSNNISKELQESIRKTVSDTSNTEYFYYAEFQYKIILKSIEE
                     FEKELDDEHEIALKLTNFGKDVLMIVEEVGYHNPCLIHYYGIVNGVYSEILQHTSQIN
                     FMITSVKKTDPSKPARRIGFIL"
     gene            complement(27604..27813)
                     /locus_tag="PC700_000844"
     CDS             complement(27604..27813)
                     /locus_tag="PC700_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580167.1"
                     /translation="MENKKIEEIEKRITMLENQLQAQLFSQITAQSNSDFIDYMYQCI
                     VLYLHEMNKSEKRKISEITKERIRK"
     gene            complement(27860..28669)
                     /locus_tag="PC700_000845"
     CDS             complement(27860..28669)
                     /locus_tag="PC700_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HDF2580168.1"
                     /translation="MNNLQIFKNKTFGEIRVIELNGEFWFVGKDIAEQLGYKDTSDAL
                     KRHVDDEDKGVGEIPTPGGNQNMKVINESGLYSLILSSKLPSAKLFKRWVTNEILPSI
                     RSTGTYNMIDLQTKLPTTYKEALQHLIEQVEVNEQLQLESKMKDQVIKELKPKADYTD
                     MILKNKGLVTITQIAKDYGMSGKEMNKILHERGIQYKQSGQWLLYKQHQGKGYTHSET
                     IDITRSNGMPDVKMTTKWTQKGRLFLYDLLKTNNILPDIEKGYSYQTSILG"
     gene            complement(28717..28920)
                     /locus_tag="PC700_000846"
     CDS             complement(28717..28920)
                     /locus_tag="PC700_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2580169.1"
                     /translation="MKKRSLKAGRIEAGYTQEELAYKIGIAKSTYNLKENGKRNFTEK
                     EMIMISYILNKTMDELFLEKRLT"
     gene            29097..29438
                     /locus_tag="PC700_000847"
     CDS             29097..29438
                     /locus_tag="PC700_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDF2580170.1"
                     /translation="MATFGERFKQLREEKKLTQDELVSKFNKVYFTSFNKSTISQYEN
                     NKRKPEINILENWADFFDVSIDYLLGRTLVRNHIDTVAAHRVNPHKDLPEEAQEQLND
                     YIEFLMNKYKK"
     gene            29450..29998
                     /locus_tag="PC700_000848"
     CDS             29450..29998
                     /locus_tag="PC700_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2580171.1"
                     /translation="MLILKKLRTEKGISLDKLSTDLNINKSTLSRIENGLREPKESFI
                     KDCSDYFGVSTDYLIGKINIDDSNKLTKCLNSTFPIRLKELRKKKELTQAELSKLLNC
                     SLSKIAMLETSKREPVKEDLLRISEFFNVSVDYLLGKTSIENYITTDEISKIIKSYES
                     LPKEAQEHINSYIEFLVDRYKK"
     gene            30074..>30577
                     /locus_tag="PC700_000849"
     CDS             30074..>30577
                     /locus_tag="PC700_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDF2580172.1"
                     /translation="MNKLDALLDLANNEEIEIYYTDKIADDIKGLYINRQGLKIISLL
                     NSLKQNNAKLIEILAEELGHHFTSVGNYVSSKNSYKNKILIDKTENKALKWACEFLIT
                     EEEIIHVINSHATSVYEIAEELQVSINFLLKRLEFLSKKKSMLDLGNNRFLVLTNLPN
                     FYIYEDIF"
BASE COUNT         9136 a         5782 c         3089 g        12570 t
ORIGIN      
        1 aatctatttg acagtagagt gaagttcata atttaaacaa ttagtaatta tttaaatttg
       61 tggaacttga ttttaaaatc aaattcccag ccacttttac tcttgccacg agttgagtgg
      121 ctttttactt ttagaaatac tttacaaatt aagcaaaata ttaaactagc aataacgcca
      181 gctattacat taagtaaaaa gttattcata cttcccacct cctttcatta ggaagtaggt
      241 tttatcccag tatgaactcc actctataaa ttgtagatta catcttcttg ctaaaaatat
      301 tataacatat aattattaca tattttacct attctatatt tattttttta ttttgctatc
      361 ttcttcgtcc cctctttctc tctctttcag cttctttcat tgcttcctct tcatcttcta
      421 tctttataag tattgaggcg gctgctaacg ctctctcatt aacttctaaa ttcatatatt
      481 cacttggctt ccactttaat ttttgaatac aataatgagt gatgctagca tcaaaatcgc
      541 cacctctgat tagttttttg cttcttctac tttatcctca aaagatgtat caaatccatt
      601 gacttcattc acttttactg tataattgac atactcacct gctgtaagca ttgtctttaa
      661 taactgagct tctcccatta ctccataact attttggagt tcggcatcct ttaaatctgg
      721 aaatactgta gatgctacac ataattcagc tacataacta ttgtagtcaa tttcacttgt
      781 atattgtcca gtatgcttac cattgttacc aatcactttt actcttttag tacactttct
      841 tcttagtgct tcgtcttctt cagatgataa aacttttaat tcccattcaa ctggtttccc
      901 ttctttatct aaaaatctgt tactcgctac atattttaca ttatcaacct ttattgcatt
      961 ttgacttaaa aaagcactta aattactcat attattctaa tctcctttta ttttaatttt
     1021 tcatataaaa aatacacatt tataacttat aaatgtgtat tttactccat acctatattt
     1081 tttattactt cttccaatgg aattttttta gtaactaatt gattataaag aaatagatac
     1141 ttaacattca aattttctgc acattgtttt aaagtaatca tttcaccttt ataattaact
     1201 ctaatactat ttcttttatt atttcgttga acttcccaag taacccatct acaatttgat
     1261 ggttcgtaat ttccattcac attaattcta tctattgtta aaccttttct atatccattt
     1321 tctatagacc agttataaaa atttataaaa tcctctagcc attcattgca tacttctatg
     1381 cctctacctc cataataatt ataatcttta ttactcttat ttgtgcacct tttaatcata
     1441 cggtgataaa ctctatataa gctatcatta gatttattat gcgttgtaga gcgctctagt
     1501 aatatttcct ttcttataca gccacaactt tgtgcttttc cttgttctaa tgcacttgta
     1561 ctagttattg ttctatttcc acattcacat tgacataacc ataaattgtg actatattta
     1621 tctttcccag acattttcaa tactgtaagc ttcccaaaag ttttcccagt tatatcttta
     1681 aatctcttat tttgtccttt ccagtttgga gaacaaccac aatgtggagg cttctcattt
     1741 ttaattgatt gctttaaatt attttctact ctctcaactt tattcccaca ctcacattga
     1801 catagccaga cttttttact atgtttattt ctccctaaat atttaattaa agtaagtttg
     1861 cctaatttaa ttccactttc aaattccatg aaatacctcc aaataattta tacttacatc
     1921 ataatatttt tcaagtataa ttcaatttta taacattcca ttaagtaatg caaaggattc
     1981 aactaattcc caatcctcaa aagtgaaatc catatcttca tctaaatact caccatcagc
     2041 atcaaattta gtaattattc cactgtccat attacaatct ttaagtacta ctgtctgtct
     2101 tcctacagca gatgtagggt cttcatttgt aacttgtatg tcaaaataaa tatcctcacc
     2161 agtttcttta tatctgtaaa gtaattctct aaaaatagaa gtattataat gaaatgttgc
     2221 acttccagta tttgtactcc cagttgtttt atttcccttt gttgttcttc ctagaattgg
     2281 aacttcactt ttattttttt ccattttagc ctctaaatct atagcttgca taaaattata
     2341 tcttttacct tctatagtta taaaacattc agctttcttt gcactaactg tatctttagc
     2401 atttattgtt tgagccatat tatcactctc ctctctaatt aactgaaaca gtcatataaa
     2461 gcttactcat agcatttatt accttaatag catcagatac tatgacagtt ttcttatcat
     2521 ttccaagctc tacactaaca tcatcagttt taaaatcttc tattgccctt atattctcta
     2581 attctttatg gtgtttaaca acatcattcc agaaacttat tcttcctgcc ttatcattcg
     2641 gaactttacc taaatacttt tcattaaata aagttgcaat atcattagca atttggtcaa
     2701 gtactctaac actttggtta cttgaaaaat cgtcattttt atcatctgta aatgatacaa
     2761 aagtatttat atcctctaac acatgaactt catctcccac cttgtggaat ataaatttac
     2821 cagtttttaa agcttcttca agttgtattt gtgtataatt aacatcaaca tcaaactcac
     2881 catcatactt tttattagta ttagatttat ttatatcgca tcctgctata gctccagtag
     2941 cccaataaat caagctagat tcaactaaat ctttatcttt aatcttattt tctatagaca
     3001 ctacaccttc ataatctgca tcacttttct tatatagtac tgtttgaaac ttagctccta
     3061 ccttatctct cattctcttt gtaaattcta caaataaact tttaatttct gttgttgtag
     3121 ccaaacaccc tagtgcatta aatgaataac tttctatttt atccaagaaa gcttggtact
     3181 ctgctcctgt cacagcttcg ccattggttc caccagtaaa tacaagtcct gcacttgctt
     3241 ctagtgttgc atccttcttc caagtgatat agtcattgtc ttgtaagtct gtaataacct
     3301 ttgctatttg agtatctacc ttcttattat ctaaaagtat tacaacatca aacttagcat
     3361 tatcatctat atttgttgta actgttactt ttaagtcatt tcctcttata ccactatatt
     3421 ttgctgtggc tatagtacaa ctggctttaa cgcctttatt taatttataa aaatatccca
     3481 accttatatt tttgaataaa tctctcaaac ctttcagctt ctcatgagta taatcatatc
     3541 caaaatactt cactgaatac ttctcaaaat catcactggt tacttggaat gcttcttcat
     3601 ctatgcccca atctaactct aaaggcattg caacaatacc tctatctgat aatgaactgg
     3661 ttgccctctt agctgagata aaatttatat atgcaccagg aagtatttta ttttgcgtta
     3721 cgaatgttcc tccacctaaa gccatctaac tcactccttt cataaattta tttattctat
     3781 cctctacctc tgagaaggaa tataactcat tttcttttaa aattgcattt aataaatctt
     3841 ttctatttac atacttctta gagttaacta tctgctcctt agtaaacttg tagttatctt
     3901 ctttgcttaa tgctttactc aaaattatca cctctcttca aaccaccgaa taactctact
     3961 gtatccatct tattggtatc attatttttt atagtaaaat agttataatc aacaaagaag
     4021 tgaagaacat tgtctataat ttcaaaattc atatttgtac ctcttactaa atctccatca
     4081 atttctatat actctaattc ctccagtagc atctcagcta tctcatttat ttcaaaattc
     4141 ttatcttctg aacgagggaa ataatgtaca tcaaaagaat ttttctttaa ttctctcccg
     4201 cttggatatg gtgtcttgct tggatttaaa ggaacaataa aaaaacaagg ttcattaata
     4261 ccttgctcca catcttcgct ataaattgta tatttttctc caaacgattt atctaacttt
     4321 actgatattc catctataat attattaagc atcaaacact cctttaagca atattaatag
     4381 ttttttctct attatcttat caacttgact ttgtagttcc atttctgaaa tagttaagaa
     4441 atgttgtcct ttaacccaac ccttgccatt tttagttcta tgaccaaatt caacatatgc
     4501 attttgttat cctaaaggct ttttatcctc tagctcttat agtttcctat aagttcggcg
     4561 tacatcatca acaaaataaa ctttatttag ttgcccagca ctcttggaga gattatattt
     4621 attcactctc tacgctctac ggaaacctat agcctatccg caatctatag gttttcctcg
     4681 gtattggcat atataattaa ttaaatattt ttccatgttc ttctatggac tatatttgat
     4741 atagatgaat atgttacagg atatatttca cttagttgtt ttattgtata tccatcagaa
     4801 tacttttttc taatttcttt tacatcctca atagataact tagctctgct ttcttcaata
     4861 atcttaactt tttctataag cttatttcct actaatccct tagaatatct acttcttaag
     4921 caagagtatg aaatacctgt tttttcagaa agctctatca atgtaatttc tttttcttca
     4981 tgttttacta atatatttgt tgacctattt ctacattgtg tctttatatc cacccatcta
     5041 caattgcttg attcataatt tccttcatta tttattctat caattgtaag tttctctgaa
     5101 tatccattac tcaatgccca atttgcaaaa ttatcatagc aaaaccattc atcacacaca
     5161 attatattcc tttcaccata tctattgtat cttttattat ttttatctgt acatctagat
     5221 attataccct tccatatact gtaaagtctt ttattttgaa ccttatattt ctttttaaat
     5281 tggtatttgt ctgttaaatt taatttatct tgttcttttt ttaaacatcc acaagattgc
     5341 actaagccac tagttaaaga gtcagttctt atttctttga aatttccaca atcacactta
     5401 cataaccaat atttcctatt tcttttaccg ctttttatct ctttagaaaa tttaatgact
     5461 cttagccttc caaatttttt tcctgtaata tctaaaaatt ttgccataaa atcacctctt
     5521 ttatacatta taaatcaaga gttattctat gtccatattc tacctacttt ataatgtaac
     5581 actattacac ttagcgttta ccgattttgc tgggttttat atgccccatt gtgttaaggc
     5641 atattcagtt ggattaacaa cttctataat ataattatta ccttgtttgt aaacaggaag
     5701 cgacctagca taagctactc cactccaacc ctctctcaaa aatcctgtat caactggtgt
     5761 ccttctaatt acttttccta agagtcttgc tgctaattct cttgctgcat ccttgcaaaa
     5821 cttatctaaa tcaatctttg taaatttctc catcttttta caaactcttt taaactctct
     5881 aaaatcaaca ctgccccatc tagccattat gctttatcct taaataactc aagtattatt
     5941 tcttgatgat ttggatatat agctgattct ccacttctta catactcttt atcatttata
     6001 ataagttttg aacctgcttt aatttctata tctggagata taaagagttt aatagtttgc
     6061 tctagcttag ctaatttccc ttctgtagca gaaactatat ttttatatga aagtttacat
     6121 ggttgatttt ctaatacaat cacttcttta ttgttagttc gttttgttac agggtcttta
     6181 attggctgat actcaactat agtacattta tctctatata acatttctat tgcttttcta
     6241 gttttactta ccatcttaag cacctaaagg ttaatatctt attcttacca taagtagtaa
     6301 gataagctat taagctatca aagcgttgtt ctggtgtttg agaaccactt cctatagcaa
     6361 aatctacctt tgtatcacct tctgatatag acttttctac agcttcaaag ttaatgcttt
     6421 ctatatctaa ttgacccata tttttcttgg taaataagaa ctctccaact atcatatcaa
     6481 cttcaatttc tttcaattca attggcatag tttttatatt acaatctagt ttaataatat
     6541 tttctatttt ttctcttaca aaacctatta accacttatc tccatctttt aatatatatc
     6601 caaaactttc aagtcttttt tctatttcat caattatatt attttccata attttcacct
     6661 actttttagt aagtttattt ttctctttaa gctgcttatt ttcttcttct aaagactcaa
     6721 cttttgacct taaaatatta ttttcagcta ttaaatcttt tacacttaat gacttgccat
     6781 actttactgc cttaccagtt tcatctatca aatcatatcc catctctaag aaatcatcta
     6841 ttttacattc ttctatggtt agtattctat ttagtttttt tacttgtggc actatatatc
     6901 actccttttt ctatgcttca acaacaaatt gtattgcatc agctttttta tttaatataa
     6961 atacatcctc aaaactttct tcaaagtaga agtattttcc ctctgtaact gctgttggtt
     7021 cgtctaactt agagaactga taagaaacag gtgtaattat tgcacttggg tgaactaagg
     7081 acataaagat ttgtttagct cctgctccta ctttccatcc agttgtaaaa tcatatgcag
     7141 ttttcattag attagatggt actttaatta ttttaactgt gtcaatatct gtggtttgtc
     7201 tatttaaaga agttccacca tcttttatgt ttactgttct ttgtatctct tttgcatttt
     7261 tgataagtgt atttactact ggagtaacat acaatattct tccattttca ggtactctag
     7321 cttctgtcat tttttccatt aacttatcaa atacttctaa tacgtttgct gttgtaagaa
     7381 cagttgtatc tgctgtgtta cctaatgcgg tccaatcagc atatatttta gatatacagt
     7441 aagcatccat ctctggaaac ttttgttcct cattatatac ttttgttata ttacctattg
     7501 aagccacata attagtttgg tttatatctg ctggatgaac caatgttgac catttccttt
     7561 gattagttaa taccttaggt tcccaagcat tatcatagtt tctttgagct actgctattg
     7621 tatctctgtt tgaatctacc cttccagttg tagatatagt tggtatttct attgttttag
     7681 aacccgtcca tctatatctt ccattatttt ctgttgcata caaatccccg aagtttaaag
     7741 tataaggata tgcttgtgct aaaacatttg aatattcttt tgcataattt agtgctgcca
     7801 ttttatttcc tcctatttat tattattttc atgaggtctt accccagtaa aattaaaacc
     7861 aaaatcattt atcttaggct cttgccctgg tgttatagta tctattttag gttcttcacc
     7921 ttctagtgtt gcattaaaca aataatcttt atcctgtttc aaagggttta tttgctcttc
     7981 aaaagctttt tgtctatctt tactatttct tagggcttcc atgtctaaat gagcttttaa
     8041 cgctatttca tctctacatt taacagattt aaaagcatca cctaaccagt aattaaaatc
     8101 tttttcttca atttcttttt tgtaggtttc ttccaaagtt ttcttatcag tttcataagt
     8161 tgtttttaga ttctctacat cttcttttgt catacctcct tcaaactttt taatagtttc
     8221 attagctgta ttaagttgtg tttcaagatt tacataatct tcttgagtaa ctgtagtctc
     8281 ctttattttc ttttctatag atttttgaag agaagctaca tcaattttgt tatcctctac
     8341 ttttattcct tctagcaatt cttttaacca atccatttta aatttctcct ttcatttttt
     8401 acaaaataaa agcacctact aatttattat ttagcaagtg cttttacata tttactattt
     8461 gtatatcttt ccataaatcc tttagtaatt taccatcaat gttgtaatta tcaaccatat
     8521 ccttaccatt tttataatac tttgtatctc cattagggca aagagtaata aaccttgtat
     8581 catcatctcc aatagatata ttatatggtt tattatataa gtcaaattct atatctaatc
     8641 ctaaatcaat agaatcaatt aaatgttgta aattcttaaa tttattatcc attttattct
     8701 ccattcaaaa tatctttgtt agctatttta tgagcttttg taagctccat atccttctct
     8761 ctttttacct tatcatggtt attttcatca gctaaccaat catgtttatg aggtacaatt
     8821 ttatgttgct ttgggtttct atggtcagtt aagtctaaat ctaatctagg ttttcctgta
     8881 ttaccatagt atcttctttg aattaattta ccatctttgt aattatcaaa tacactattt
     8941 ggttttgatt caaatggcac tgaatgaaca cttccactag ttaaatttct ctgattctta
     9001 acttgccaat ttacatcctt ataaagcttt ttagcttcct cataccttat agtatcatta
     9061 tacttcatat gttgatattc atcaaattta gaaggtattt catttcctaa taccttttta
     9121 tattcttcaa attgttttct atctttactc tcatttagtt gcatttttct aagggtgtca
     9181 gctttttctt ttccaagtct accctctata tgtttctcat accactcatt atacttcata
     9241 ctagatggta cataatatgt ttttccatct tctcctcttg ctgctctata gccttcttca
     9301 tcctcgaacc aaggagctgt tgttgtccta caacgacaat gaaatggtgg agctgttatt
     9361 ccaacttgat aatctttcat atcaaatatt tttccatcta actctctgca tatatttgaa
     9421 gttcttaaat ctaatgtagc aataatctca tatttctcta catctaaatc actaaaacaa
     9481 tcttttctac ttgctgatgc aaagaaagct gattcagtca ttatcaaatt cttagcttgt
     9541 gatttagata cattaaatct cttagcaaag tcatttacta gattttttgg attttcacct
     9601 ctaataattg attgagttag cttagtatgt agttcattta ttaaagtagg tctatgctta
     9661 ccccaaattc tttcactaaa gtttaatcca tctgttgccc atggtttaga gataatttta
     9721 tttattctat tagtatcaag actcattaaa ctccaaccaa cgtttactcc ttgttgaaca
     9781 ttaaaagctg tatgatagta tccacttgta taaatatctc tcattagttt atcaatacca
     9841 tcaagttcat ttccatataa aacttctacg tgttgctgta tttgtaactt taaagcttca
     9901 agccttgtta tatgaagtct tgcactagca ttttctaact ccttcatcca cttttgattt
     9961 atagcatttt ctttgccata tttaatatac tcttctacat tccacttaaa ttcttctaat
    10021 tctcttgtat ttaacagttt cttagcttct aataaagata ttccttcatt ttcagcaaat
    10081 ctgttatacc atgataatat atctttttct atactattca tagctaattt gtatttcttt
    10141 tccaattcaa gataatattt tacactttta ttgttttgaa cttcttctaa ttgttcaaat
    10201 cttttcctcc aataatcttt atgtttcatc tataacaccc tcttgattat tagtaggaat
    10261 taaatcatca tattcttttt gggtttcttc ctgtttttta agtctttcaa gttcgtcatt
    10321 aacatcttca acccaaggat gattactaac aatagtttca tctgatataa ttccaattga
    10381 tttagaagcc atatctattt tttcagactc atttattatc atagaatgat taaatgttat
    10441 ttgaactgac ttataatcat agctcttact accacttatc tttaaatact cacacacaaa
    10501 ccataaaagc tctctaattg cttttttaaa cttcttttca gtcttggaac attttaaatc
    10561 aagtagtgaa tataaaaatt taagagctac acccgatttg tcacctgtgt tttgagattc
    10621 gggattaact ccttgaccaa aaataattat attcttttct aatctatcaa gaagctcctt
    10681 tttggcttca actggtatat ttatctctag tttatcaact ccacctccac catctacttt
    10741 aattgattta tagtatctta tattatctat aaactcttgt agacttgttc ctggatactc
    10801 ttttaataca taaatgacct cttgcatttc atctaaattg tctgcaagtg tagaaatgtt
    10861 attatcatat atatcaatta aagacttata gaaagttaaa tctgagacac acttttcatt
    10921 atttttaaaa ggtataaatg gaactttacc ccatccctgt tctttgttat ttattctaaa
    10981 atgaccttct tgtatatcag tcatttttcc atattcatca tataaaaatt cttgaataaa
    11041 actattacct ctttcaataa agtaagttac gtcattttct gtgtagtact caactctttt
    11101 tattttattt ccatctatat cttcaatata ataaaaccta ataaatgcaa ctaattccct
    11161 ctgtctttta ctatcccaaa taggaattgc ttcttcagct ggaattatta catatttaaa
    11221 ctcacctttt ctattaatat atggatgtaa ccattcaacc cctttattac tagcattgag
    11281 atatagttct gttattgtat cgtcaaactc ttctcctagt aagtcattta aaagcttagt
    11341 gagattatca tcatctgcat taaatactat gggatttccg acactatagc ctaccttttg
    11401 gtcaactaaa agcttatggt agttgttaat tgctttatta ttaactttag taaaatcatc
    11461 aaccttagct ccatctaaga gataatatct tctcttattg tttacatcag tattaccata
    11521 atagtattct tctccttgtt tatatttttc tggcctatgc tttaagatgt agtgttctat
    11581 gacttttact aggttaaagg tgctctcttt ttttaactga acttttatta aatctgtttc
    11641 acttatataa atatttaaca cctcctttac tttaagaagc ttattccatt atttttaagc
    11701 ttattatcta tagaatatct aagagcagcc attgcatcat ccataaactc aactggttca
    11761 tcaagatata atccagttct ttcgtcttgt ttccatttcc attgttgtat ttcttttatg
    11821 gtattagtgc aactaggatg tacatgtatt cttaattgtt tcaaataatc tatttgagct
    11881 ttaacacttc ctggtccttt tttaactcct ttagctttat atcctgcact cttccacatc
    11941 ttaattctat ctggttcagc actatcacag tacataaata gagtcttttc taaacctcta
    12001 ctatttgcaa tttttatgat ttctgaggta tccatttcat gtacatatat ttcattacat
    12061 atatataact ctccatcctt aaagccaatt ctaagtattg catttgcatg attaaagcca
    12121 aagtcttgtg acagtctcat attgtcaaag tattcaaatt ctgtgggaaa ttcatgtata
    12181 acataatttt taagtattgc tccaccagtt tctccccatt ctccaagacc atatactttg
    12241 tacccttctg ggtcttgctc ttttctcatt tgcattcttc tgtagtaagc ttcatctatg
    12301 aatctatttt gtagataagt actatgatga gtaaatatat catcattttt atagtcaaaa
    12361 tactttcttt ttatccaatg agtagctgag actggattaa atgtaaatgt catttgataa
    12421 tacaagttag gattagttaa aatacctctt aaacggtcat ctagtatatc tatgtcactt
    12481 tccataagtt ctgtagcttc ttcacaccaa acccatgtta attttccttt cgagaagtta
    12541 attgatttta atttttctct ttgttttgca tcattaactc ctctgaaaat tatagagtta
    12601 ccagtaactt tactcttaat ttctaaagga tttaaagtag ttttccaata cttatcagct
    12661 tgtttaccat aaatacgatt tatagctcct gtaagctctg catacgttga atacttatgt
    12721 gtagcttctg actttctaac tactaataga ttagctcctt gatacttctt atctcctaac
    12781 tttagtatat agtcttgtgc tacattaaca gattttccac tccctgctga acctttcatt
    12841 gctctgtatc tttttttagt aaaattagct tccttgaaat ctggattaaa atttattcta
    12901 actatcattt ctatcaccat aatctacact tattttcaac tcatcatctc caatatcatc
    12961 tttatttagg ttagcaacct cacatttcaa cttctcaact cttgttttct gctcctctgt
    13021 agccaaattc caatccttat gaatcatttc atcatactgc ttaattaaac tccttaactc
    13081 actcatagct ctactctgtg cattaagaaa agatgcttgc ctatcccatg caaattgaaa
    13141 ttcatactct atcttctcac tattttctgt gatttcatgt ttctttaatt ccttaatcat
    13201 ttcctcttta tctttaacat acattatctt ctgtgctctt attattgctg cgtattgaat
    13261 tgttatctgt tcccaaagaa tatcaaattt atcttttata gatatttctt gtattaattc
    13321 cctagtttct tcaggtaagt attttgagaa gaaaccaaac ttttcagcat ttttattctc
    13381 ttttggagca ccataaccaa ctgaattttt attagaaaag ggtgcacccc ttttatttat
    13441 aggtgcaccc ttcttttttt cactagacca attgtatctt tttatccatg actttaaagt
    13501 atttaaacta atgtcatact ttgctgatat ttccttttgt ttcattcctt ttaagtaatc
    13561 ttgttttacc ttttctttga catcttgcac atcaccacct ctttatttgt ttgttttggg
    13621 aataaaaaaa caatcctatt taataggatt gtttcaaatc tagtctcctc tatccttcca
    13681 ggataaatga aacatactta ttattaatgt agttagagat aaaattatat gaactgtaaa
    13741 aaatattaaa acatagaaaa ttatattaga ataatatcga gtaagcatta ttgtattgat
    13801 aaaacatatt acaacaacca aaatctccat tattacagta taatatgctt ttaactgtat
    13861 gaattgaaag tatgatatat taaatccatt tacctttctt tctgttttta tatcttttgc
    13921 ttctgatatt gatttgttat atgtagtaaa caataatgta ataatgcaaa ctccaaaagc
    13981 tactaatagt cctgacaaag ttagtacatc tttgtttaaa gaaattacaa cttctttatt
    14041 aattgacgaa ttaaaaaaca tgtatgaaat aatacatatt aatactggtg ttaaaactaa
    14101 ctctactact tctttagaat ttatagcctt gtaataattg agtaagatat ttaatacctt
    14161 tttatacatt ttaacacctc cttagcaatc tatgcttcac tcatagcaac ttcttgatac
    14221 attaaattaa aaaaaactga attattctca attaaatcat ctatcaaatt attcatcttc
    14281 tcaaaaatgt cttcactatc tatatgatta tctatatcaa gagaaacctc tatttctcta
    14341 cacaacttta taccctcagt atccaacttt aattttctct tatttttctc tccctcaatt
    14401 attatcctat ttattttttt atttctaaat tcctctcctt taatatattt ttcgcaacat
    14461 cttttaactt cactaggtaa atatctagtt ctaaatgtgg gcttatatat caaatcgtta
    14521 tgtcgtcttg aattatttaa attagaaaac aaattatctt catcagttcc aaacttctct
    14581 ctatcaacat ttaaagttat ttttgaaaca ccatctaaat ctaatatttg ttctataaaa
    14641 tctttacttg ctatttgtgt aatattaatt gatgtttttg acatattatt atcaaattta
    14701 ttattttcaa tcatatcctc ataatataga tgtaagtttt tacttagttc aatcatggga
    14761 acccccacaa aaactctttc aaataatact actgctatat tattttcctt cataaattta
    14821 ataaataggt gctgcttatt gctatctcct tgatccattt ttttattacc ctctacttct
    14881 ttagtatgag catttataat atttgtattc tgattgtatt tagtatattc aagtttcacc
    14941 ttgattatta aatcatcctc gctacatttt acttctgtaa tccaaaactt tttatcttta
    15001 gtaatattaa ttccattttt gttttcttta caatattctt tatccttaaa gaaggtcttt
    15061 attacctctt ttaaaatttc ttcatctgaa tagacactcc cattaatttt atttataata
    15121 tctattctat aaacatcaat atatcttttt ttaaaattac tcattatacc attattcctt
    15181 tcacaaatat ttataatgat ataattctat ataatcagtc aatatccttc aataatcatt
    15241 cgacaaccac aaaataagtc taaataatac ttttacctta ttcctgtcta taatgtacaa
    15301 atatatttca tttttaattc attacataaa aaaaaagacc ctctattaat acagtcattt
    15361 aaatcatttc gattaattct ttaatctttt tatatacctc tttataattc atacctttat
    15421 ctatcaacct aggtaatttc atagatataa ctctttcaag tacttgtatt aataaaaaaa
    15481 ctcttgtatt tcatctgtaa aatcttttct gaccttttac cttctgttct tatatccagc
    15541 cattcttgat ctttttctgt ggcaatatag taaccatgta ttctaatttg tttaagtgct
    15601 tttgtaaccc acttcgtaaa tagttttgct tctgctttat tacttctaaa tgacatattg
    15661 tacacagctt cttctgtaac aaaagtagta ccaaagttag gcaatttatc tttaaagttt
    15721 ctagtgtagg aatctccgac agtagactca ttaaatttct ttttatattc tctatctata
    15781 tttcttaatg tatcacgaat atttactatg cctagttcct ctcctacgtc atttgcatta
    15841 aaccaaactt cttctccatt ttttgaccat atcatcctta catttttctc ttgtaaaatt
    15901 ttcaacatac tactacctcc taaattagtt attaataaag aatattcaat ttgaacaccc
    15961 ttattttcga cccttcaata tgagagatta aaattgtata aaaaaagacc tagaaatcac
    16021 tctaagtcat gaattactca tttatcattc tccttattta caaaataaaa aagactaagt
    16081 tggggtaact tagccttttt caagggggaa tatattatac acttgtttca tactaccatt
    16141 ataacagggg ttaaatatca ttaaaatatc atctttttat catgtattta tcaaacagtt
    16201 aatttcaagc catctatacc aaataaatac actccaagtt cttttaccat ttcattaatc
    16261 caacgtcttg atgtaactac tccacaattt aatatctctg ctatttcttc atatgttttt
    16321 tcatcacaat agtaaagctc taaagctcta tacttttcat aagaatgtaa acgctcttgt
    16381 cttaatttca aagttttcat tgctgaatct atatgtgcta tcataataat agttcttgct
    16441 ttacttttct ttatacttaa aatatacaac tcttcattat caagcttacc taaatcatcc
    16501 tctaagtaat ctacatcttt agcttcactt accgctgagt ctatatgtct cttaaaatca
    16561 ttataatgct tcatcaataa tctagtatta tataaaacgc cttttttcct atcttctctt
    16621 tcttcttttc ttaactctct tacaatttct tttatacttt ctttatccac ttaaatcact
    16681 ccttcttgct ctcttcgaac aacttctact gcaatactca actttagatt tatccaactt
    16741 ataaaatctt ctaccacacc aagcacatct tactatttta cctgggctta taagttccat
    16801 cttttctttt tactttcata ccttcactcc ctatattctt ttaattttgc ttttactgct
    16861 tcaagtaatg cactttgccc tttatcctta ttctctatag cttccattac ttgttcatct
    16921 attgtacctt tacagattag atgatggata ataacagttt ccctttgacc ctgtctataa
    16981 agccttgcat tagcttgttg atacagttct aagctccaag ttaatccaaa ccaaacaatt
    17041 atacttccac ctgattgtaa attaagtcca tgtcctgtac ttgctggatg acaaagtaat
    17101 aattgtattc ttccattatt ccaatcatat atatcttttg agttttctat tgttcttggt
    17161 ttcaaactct taaactcttt cattaaacga ttataatcat gtttatagtt ataaaaaact
    17221 ataataggct taccatttga aacatctata atttctttta aagcttctaa tttttctcta
    17281 tggagttcct ttacattttt atcattatca taaattgctc cattagctaa ttgtaaaagt
    17341 ttatttgctg ctacagctgc tgatgaagct gttattatat ctctatctaa ttctaatatc
    17401 ttctcttttt ctaattcttt gtaatattta agtattttac tatcaaggtg tatttcaatt
    17461 ttattatcta tttttctagg catattcaaa taatcttctg cttttaagct tatacaaata
    17521 tcttttattt tattatgtat tgcattttct gctccatttt taggttgcca attatatata
    17581 gctcctgttt gataattttt ttgtcctggc tcaaagtatc gttctttata tcctgtaata
    17641 gttttaccta gtctttcacc tctatcaagt aagtacatct gtgcccataa gtctattaaa
    17701 ctatttggtg ttggtgtacc agtaagccca actactcttt tagttaaagg caatactttc
    17761 tttaaacttt taaatcgttg tgctttattt gatttaaaag aactaagttc atctatcaca
    17821 accatatcaa atggccattt tcttttataa aaatccacta tccagggtac catttctcta
    17881 tttattatat aaatatctga gtctgtactt aaagctctca ctctatctaa tttactacca
    17941 agaactttag atacttttag atgctttaga tgttcccatt tttttacttc actactccag
    18001 gtatctcttg caactcttaa aggtgctata actaaaactt ttgatatatc aaagtaatca
    18061 tacattaatt cacttatagc tgttagagta cagactgttt tacctaatcc catatctaat
    18121 agtaaaccta tattattatt atctatagtt tttctaattg tgtattcttg gtatggatgt
    18181 ggtttaaatt ccatccctta acctccttga taaaatcatc tatctctttt aatgtgctta
    18241 cacacttaac tttaaatcct aattctctta attctcgcat tttatattgt tgaagcttcc
    18301 ttagtttttt acctggtgct ttaagttcta caaatataac atgtccttct ggtaataaga
    18361 caatcctatc tggcacacct gcctcacctg gtgaaataaa cttcatagcc tttccaccta
    18421 acaactcaat ctctttttta agtctttttt ctatttttga ttctaacaaa atatcacttc
    18481 cttaaaattt aagtgtcaac aatgtcaaca gattttctat atatatgtat atatacatat
    18541 taggcataca tatgtatata cgtatatgcc taatattact tatactactt tatacttaat
    18601 ttttgttgac attgttgaca ttaatacctc aaacattgta attactgcat cttaggtgtc
    18661 aacataagcg tcaacaaagc catttttttt gttgacattg ttgacacttt taaaattttc
    18721 aaatttactc tgtttttaat tctgttgaca ctaactttta cgtacataag ctctctgcct
    18781 accatatatt ttcccaaacc ttaaatttcc attatatgat atccaatcat ctaatccttt
    18841 taatatatca ttaatttctc ttgataagat aggcgtgagt tgtttaggtt ctccattaaa
    18901 tagctcaacc catatctcca taacacacgt tttttctctt aatattgttc cttcttttaa
    18961 atcaccaaaa tctgaaccat gaatatattc tctcttttct gaaatactta aatcatacca
    19021 attctcagta ataggtttat ttagatactc ttcgataatt ccagatttag cattttcttc
    19081 tgagtgagtt ctttgttgtc tttcagcttc ttttttctcc tcatctgata gatatagctg
    19141 ttcattggct ttacataatt ctactgcttc tgcccaaatt tgatttcttt cattatcaag
    19201 ttgaccatta aatatactct tgttaacctt ctttactcct gtatctattg gccaaaacct
    19261 tctattcccg gtcttatctc ttaaaaattc tttatcatta gttgttccta taactacaca
    19321 ttgacgtaaa aaccttgatg ttctcttccc atatgcgacc ctatatatat cctctgattt
    19381 gcttagaaaa tgcttaactg cctcaatatc tgctttttta gtagccatca tttcacccat
    19441 ttccagcaac catactcctt gtaattgttc atacgcttct ttaccttgta cagtagttaa
    19501 actatcagaa taccagtctc caccaagttt tttaataaaa gtactttttc ccattccttg
    19561 aggaccagat aaaaccatca tattatcaaa ctttattcct ggattaaata ctcttgctac
    19621 tgctgctact aaaacttttc ttattatagt ccttgtatag tggttatctt ctgcacctaa
    19681 atagtcaatt aaaagtgtat ccactctctt aatgccatcc cactttaaag aatttaaata
    19741 atcttttata ggatgaaaag tattattttc aaaagcaatt attagagcat catttacttt
    19801 tgatggtgat gagatattgt aaattgtttc tatatgatgt ctaagcccag aatcatcact
    19861 atcattccaa tcatttaatt tatcatcttt tctccaaggt aacttaccta aaacgacagc
    19921 tctatttgaa aattcattat aagctatttt tcctttcaaa tatgggtcat tttctataaa
    19981 cattaagata ttgtttgttg ttttcttata acttccctta ttgtcataat ctaacctagt
    20041 taaccattca tcatcttcaa aatctatatc accaaaatca tccttagctt tatcaaggtt
    20101 ttctcttcct atagtctttc gtactttagt gtcactgctt gcaaattcgc tcattcgagt
    20161 aaatgaaggt aatctattta caggcgtttc gggtttagca tcttcatcaa gttcaccaaa
    20221 tttatgtatc ctaactaaat caaaagcatt gcataaaatt ccgcttgctg ggtctgtacc
    20281 atgatgacta tatgaaaact tgtcatcata aataactact ccaccacttg tactaccttc
    20341 tgaatatgta tacctggttt catcaatacc aggaatatat acttcattta agaaattttc
    20401 tatagtttct tttatactat aggacctgca aaatgcacct ataattccag ccttttcaat
    20461 agggtcttgt tgttttttta attgtgtatt aaacttttgc ctctccctcg aactttctgg
    20521 ccaatagctt acatctgtcc agtctaaata taaatctagt atttcatttg ggtttaaaaa
    20581 ctctccatct tgaaatttaa atatatagtc tccatcaatt gaagtacttg gaaaatacat
    20641 aagtctatga ggttgatatg tagtatcatc aaacatatct attcctatag tatctgctat
    20701 catcctggaa attgcttgat attcttctgg tagtactggt ctagttaaag gtattacaag
    20761 tctatatctg ggattattct cagtatgtga gtgcgtagaa tacataagac aagcataatc
    20821 atttaataat gttatatcct cccatatatc tttattcgca taatctatat ctaaagttat
    20881 aatacttcta tttgcaatat tctctgcttt tcgtcttcca ttttttaagc ttccacctac
    20941 aaatccacca acatctttaa ctctatcttt ttcagtcttt gacatcttcc tatattctgt
    21001 atatgtttct tgagttctta atgttttact taatctattt acaagttcgg accataaaat
    21061 acttttattt ttccaatgtg tttctaattt attttttcct atggccagca tgagttggcc
    21121 atcatgtctt acatttatgt gttcaatttc actggccttt atatccataa atcaatcacc
    21181 tagtctttct tataataatc acattcatat ccatctgctt taagaggaag acctttagcc
    21241 caagatattt cttttcccat aatactgtta acttcttcta aagaacctcc ttttttatct
    21301 acatcaatta caagctcatc atgtacatgc attacaatgc tataacctgc atctgtcaca
    21361 ttaaacatag cctctcttaa gcaatctcta gctgtagctt gaacaatatt ctcaactaac
    21421 ttaggtccat aagtatctat tcttttccat tgtttacttg tctgttccat accttcatat
    21481 gttatcttat ccccactaaa tgtagtatga ggctctatct taggtcttag atatgataat
    21541 cttctaccac ttggtagttc tataaataaa actcctggat tataaataaa tttaatccca
    21601 tgttggagtt ttactatagt cctatctttt atggcttttt tagctgcctt atctacatcc
    21661 caccaaaatt tagttatatt tggattagca tttctccatg ttgttacaag aggttgaagc
    21721 tcctcttcat caagacccat ttttatagcc cccattgatg ttaaagctcc tatgcttccc
    21781 ccatatccaa gagctagctc tgaaatctta cctttttgtc taagtgggtc acctttttta
    21841 atactttcta ttggaacttt aaacatatga ctagcactag cttcatatat tttcccgtga
    21901 gaattaaata catctaatct ccacttctca ccagcgaacc aagctataac cctagcttct
    21961 attgcactaa aatctgatac tataaatcta tgaccttcac ttggtataaa agctgtcctt
    22021 atcaattgac ttaagacatc tggtacacta tcatataaaa gctctattaa atcaaaatct
    22081 ccttctttta ataggtttct agctaggtct aaatcctcta tatgattttg tggtaaattc
    22141 tgtacttgta ctaatcttcc tgcccatcta ccagtcctat tagccccata aaactgtagt
    22201 agacctctta ctctattgtc attaccttta gctaatttca tagcctcata tttctttata
    22261 gaagttttgg acattaattt tctaagttct aaaattctaa ctacattttc atcatcaact
    22321 tgttttaata tttctggaat actttctttt gttaggcttg taatctcaaa gccaacttta
    22381 tcacttaacc atttttttaa ttgagctgga ctatttggat tatttagacc agttatttta
    22441 attgcttctt ttgtaagttt ttcagtatat cttttatcac attctattgc attctctatt
    22501 aactctgtat caactttaat accagtatca ttaattctct ggtctaaata ccataattta
    22561 atttctcttt cggtagtctt atacttacta agtttgtttc ttatttctct ttcaactaca
    22621 acatcttgtt tacaatattc tttaaattta ttccattttt ccatatcatg tattggtaaa
    22681 tttctagttc ttcccttgtt aacttttgta gccttacaag gtttacagaa atattgtatt
    22741 aatgctttac cttctttcat tttctgttta tcttcattaa actttaaagc cttagacaca
    22801 ctatctaaac ttcctggaag tcctagtgtt aacgccttta tcattgtaca tgaccactca
    22861 tttggtttta aattaatatt taaaaattta cttattgctg ttctttcaaa attagcatta
    22921 aatgctgatt ttataacttt attatcattt aaagcttcta ttacttcttt tggtaactct
    22981 tcatcattta ctaaatcaat aacttttacc tcttcattat caaaagcata ggcaaataac
    23041 agtatctcaa aattagcaga gtctacatat ctgtagactc caactttttt tatatctaaa
    23101 tcactatatg tttctatatc aattgataag gtcctcatta acttaagaag tcctcttctt
    23161 catcttcata ttcaaagtca tctgagaaat ctgcttctgc actagctcta gctcctccaa
    23221 gtacttctcc atctgctaac ttttgaacat tttgtaagcc acaacctatt cctttatttc
    23281 ctgcactatt atatgggaaa aagtttatac taactcttcc ataacaacca ctatatacct
    23341 ctgtattatc taatatttca tttaagtctt tatcaactat tcctggcttt tgtgtactat
    23401 ttgcatttaa aaaatacatt cctacatatt cctctgcttc atcagctctt tctgcatctc
    23461 catcacgaag aggtgttttt aaattacctg gtaattttcc accccattta gaagttttac
    23521 cttgttcttt agctgcatca atagccttct ttattcttcc taaagtcacc ttatctgatt
    23581 ttggtattaa aatacaaact gaatacttgg gctctgcacc ttctaccatt gctctgcttt
    23641 taaagatatt gcaataactt aatcttacct ttcctgttac tacctttgtt gattgtactg
    23701 aattactcat aattttcttc ctcctaatat ttttatattt ggtatattga ttttatattt
    23761 gaagttattt ctccaaattt atctaaatct ctaacttgct tactaaacaa tttaattctt
    23821 ccacaaataa ctgttgaaga aacatttaat aactctccta tatcagatat ctttttatct
    23881 tgtaaaatcc aatctactat tttatcaata tcttttaaat atgactttct tgcaattttt
    23941 cttatataat caacatcata gttaaattga atttcataaa atccatgtat atcttttaat
    24001 ggtcttttac tatcttcaaa tacacttaca tctaaacctt ctatagagtt tctataatgt
    24061 ttttcgccat aacgcaaagc tttaaacatt tctattctta tgtaagaaac agccactgta
    24121 gaaaacttgc ctctgttact atcataattt aatgctgctt tatataaccc aatacatcct
    24181 tcttgataaa actcttcata taagtatgga tgttgctcta cataagactt aaaaaatcta
    24241 tttatactaa aatgtacaag attcaaatta ttttcaacta gtttagttat ttcttgatta
    24301 ctcataaaat tatatcccct tttaaatctt tttatagcta ctaggaatta aatattttta
    24361 attatccttt gctcttactg atacaaagca tactggacta acttttatat gttctggtat
    24421 atacactcta tcaagatgat tgtcatttac ttcgaggaaa gttgacatca ctttatcctt
    24481 tattaaactt tctcttttaa aatccatcac tataactttt gcattttctt cagttgagtt
    24541 atattctgta atatttagtc tctttaacat atctagtaaa ttatcttttt cataatctaa
    24601 atctttttcg atactctttt ttctatcctt taaatctaaa attatactta tgctttcatc
    24661 taatttatct tttatacctg catctaatgg catgtaatat cccccattta tatttcaaaa
    24721 tcttttttcg ctgaatctat actattaatt tctgaccttt tatcactttc tactactaaa
    24781 gtagcttttc caactggttt tataattaaa tcacttagta gtttagcaaa actcttttta
    24841 cctatagctt tctccatatc actaattcct tttaaggtcc ttggcttata aattttttct
    24901 tcatcataat ctgaatttaa taaaacctta gcaacttctt gctcatctac atactttcta
    24961 ttacttctgc cttctacaag cttatatcct ggatatttaa caccatgctt ttcagcttgt
    25021 tctagtgcat aactttgaac atcctttaac caatcttgta tattcttagc aaaacctaaa
    25081 atatctgcta tctcatattt attaagagca aatgtgtcag caaaatcata ttttctagct
    25141 agtttaaggt tatcctcagc tctttttctg caatcatttt tagctctaca aaatccacaa
    25201 tgactgccac ttacaaactc tccttcacca ttaaaagcca tttgagcctt cttcttaaca
    25261 ttatctgccc atgtaagtaa tttagtaact tctatttctt cacttgatat gttatcaagt
    25321 ctaggttgaa ttattgttgt tttaattaaa tcaatatcgt atagcatttc aaactgatta
    25381 tatgccccta aaccatatag tctaagttga ggattctcta tagctgaaac ttctaaacct
    25441 tttccatact ttaggtctat aacttgaagt ataccatcag atattacaac aacgtctcct
    25501 gttccaaatc cttctggaac ccactcacta aaatcgagtc tttcttctaa catcactatt
    25561 acatcatcac atatagcttt actatcattt actaattcaa ctacattctc aacataagat
    25621 tgtatatagt cctccatttc agagttataa tactcacttt tctgtatctt tttaattctt
    25681 gcattatatg cctttttact tattttttca tactctagca ttaatttaac ttctgctaat
    25741 tcatgtgcaa ctgttccttc ttctgcatat atactagtta atggtggata gttttcttct
    25801 aattttatac taggagtgca gtgaagccat ctatgggctc cactcgcact aagtcttgca
    25861 tgttgtaatg gcatttatgt accccctata aactttctaa tttgttcata aaagctgaat
    25921 aatcttcttc ttttacttca cttaacttac tagctccaaa ttcaccaaat aactccttaa
    25981 gcttatcctt cttacctgcc ttacttactt gtgctgcctt agttcttacc tcttcttttg
    26041 tgtatttaac ttcactagtt gtattttcat tattcttttc tatttcttcc tttgcttctg
    26101 ctatttcttc tttaacgtct tcttttactt ctaccttttt aatcttttca gcttttttaa
    26161 cttcaacttt cttctcttct ttcatatctg ttatatttat aggttctatc tgcattgctt
    26221 ttcctagatt aagacctcct aaagcattcg ccactactaa tagtgcattt gtaaattctg
    26281 gtgcttctac tttaactttt acatttacat taacttcaac cattttaaat atctcctttt
    26341 tgtgttatac tttacttgtg ttatatttta ttttttattt ttttgtgtgt tggttatttt
    26401 accaacactt ttttatttaa tattccaacc gatattttct taccagtttt aatatcttta
    26461 aatactatat ctgctataac ttttccatct tttttaagag ttactacatt cttattatta
    26521 gtatcaagac tcagcaattt catcccccca ctctactgct tttaaaagtt catccaaatt
    26581 tttaccttga tttctttcaa taaaatcatc aacttcatat cttgaaattt ttctaccatc
    26641 acctctaact agtgatttta tcaaacctgt gcttactaac ctacgcataa aagctgtatc
    26701 taactttaaa attccccttg cttcttctac tgttattaga taatttggat aacctctttt
    26761 tattaaaaca actatatctt taggctccag tacctttacc ttttgttcaa tagtttgact
    26821 cttaactcta tctgtctctt gtttacttat ctctatttct attaaacttt ttaaactatc
    26881 actaaattgt tttgctatac tctcagaaat atccatgtga aaattctctc ccctcttttt
    26941 aattatttat cttccaacta attcgtctaa tgtaatatct aaataatcag ctaattttat
    27001 tagagtatct atagttggat tagaactttt atttctcagt aatttatata aaccacttac
    27061 atctatattc aattcttttg ccaatttata aggttttata tctctttttc ttagcatttg
    27121 ctttatattg tcgcctatta acattgtatt cacctcagtt ctacatggta taatataaac
    27181 atggaataaa ttccaaaatt ttcaaaagga tggtgtatta tgcagttttc aaacaatata
    27241 tctaaagaat tacaagaaag tataagaaag acagtctctg atacttctaa cacagagtat
    27301 ttttactatg ctgaatttca atataaaatt attcttaaat ctatagagga atttgaaaaa
    27361 gaacttgatg atgaacatga aattgcacta aaattgacta actttggaaa agatgtctta
    27421 atgattgttg aagaagtagg ttatcataac ccttgtctaa ttcactacta cggcatagta
    27481 aatggagttt attctgaaat tcttcaacac acatcacaga taaactttat gataacttca
    27541 gtaaagaaaa ctgacccttc aaaacctgct agaagaatag gttttatatt gtaattatca
    27601 ttcttatttt ctgattcttt ctttagtaat ttcactaatt tttctttttt cacttttatt
    27661 catctcatgt aaatacaaaa ctatacattg gtacatataa tctataaaat cactatttga
    27721 ttgagctgtt atctgactaa ataactgtgc ttgcaattga ttttcaagca tggttattct
    27781 tttctctatt tcttctattt tcttattttc caattcaatc tctcctttct aaaatattct
    27841 gtatttagtt ttcaaagtac taacctaata ttgaagtttg ataactatat cctttttcta
    27901 tatctggtaa tatgttattt gttttcaata aatcatataa aaacaatctt cccttttgag
    27961 tccacttagt tgtcattttt acatcaggca ttccattact tctagttatg tctattgttt
    28021 ctgaatgagt gtatcccttc ccttgatgtt gtttatataa aagccattgt ccactttgtt
    28081 tatattgaat ccctctttca tgaagtattt tattcatttc ttttccactc attccatagt
    28141 cttttgctat ttgagttatt gtgacaagtc ctttattttt taatatcata tctgtataat
    28201 ctgcctttgg ttttagttct tttattactt ggtctttcat tttactttct agttgtaact
    28261 gctcatttac ttctacttgc tctataagat gttgtaacgc ttctttatat gtagttggta
    28321 gtttagtttg caaatctatc atattatatg tcccagtact acgtatactt ggtaatatct
    28381 cattagttac ccaacgttta aataattttg cacttggcaa cttagaactt aatattaaag
    28441 aataaagacc actttcatta attactttca tattttgatt accaccagga gtcggtattt
    28501 cacctactcc tttatcttca tcatcaacat gtcttttcaa agcatcagat gtatctttat
    28561 atcctaattg ttctgcaata tcttttccaa caaaccaaaa ttctccattt agttcgatta
    28621 ctcttatttc tccaaaagtc ttatttttga atatttgtaa attattcata atttctttcc
    28681 tccttatgat tgtttttgtg aacaataaat tttaatttat gttaaccttt tttctaaaaa
    28741 aagttcatcc attgttttat ttaaaatata gctaatcatt atcatttctt tttccgtaaa
    28801 atttcttttt ccattttctt ttagattata tgttgatttt gctattccta tcttatatgc
    28861 tagttcttct tgggtatatc ctgcttctat tcgaccagct tttaaacttc ttttcttcat
    28921 gttcttcacc ttccaatgtt ttatttttgt gaacttcata cttttattat attcacacaa
    28981 acaaaacttg tcaatacata agtttatttt tttgttaact tttttatttt tttctaaaca
    29041 taagtttacg tttctgtaaa cttatagtat catttaatta ttgggaggtg ttgtttgtgg
    29101 caacttttgg agaacgtttc aaacaattac gagaagagaa aaaacttact caagatgaac
    29161 tagtttcaaa attcaataaa gtttacttta caagttttaa taaatcaaca atatcgcaat
    29221 atgaaaataa taaaagaaaa ccagaaataa atattctaga aaattgggca gatttttttg
    29281 atgtatcaat tgattatctt ttgggtagaa ctcttgttag aaatcacatc gatactgtag
    29341 ccgcacacag agttaatcct cacaaagatt taccagagga agctcaagaa caactcaacg
    29401 attatattga atttttaatg aataaatata aaaaataagg attgaatcta tgttaatatt
    29461 aaaaaaattg agaactgaaa aaggaatttc tttagataaa ttaagcacag acttaaatat
    29521 aaataaatcc actttatcca gaattgaaaa tgggttaaga gaaccaaaag aatcatttat
    29581 taaagattgc tcagactatt ttggagtatc tacagattac ttaattggaa aaattaacat
    29641 agatgattca aataaattaa caaaatgctt aaattcaact tttccaataa ggttaaaaga
    29701 attaagaaag aaaaaagaat taactcaagc agaattatca aaattactta attgttcact
    29761 aagcaaaatt gctatgttag aaactagcaa aagagaacct gttaaagaag acttgttaag
    29821 aatttcagaa ttttttaatg tgtcagttga ttatctactt ggtaaaacct caattgaaaa
    29881 ttacataact actgatgaaa tatccaaaat aattaaatct tatgaaagtt tacctaaaga
    29941 agcgcaagaa catattaata gctacataga gtttttagtt gatagatata aaaaatgaat
    30001 atttagagca gttcactcct gctctttata tataaaaaag taacacatac attcttttta
    30061 tagggggatt tcaatgaaca aactagacgc acttttagac ttagcaaata atgaagagat
    30121 agaaatttac tacactgaca aaatagcaga tgacataaaa ggattgtata taaacagaca
    30181 aggactaaaa attatatcat tacttaattc attaaaacaa aacaatgcta aactaataga
    30241 aatcttagca gaagaattag gacatcattt taccagtgtt ggaaactatg tatcttcaaa
    30301 aaacagttac aaaaataaaa tcttgataga caaaactgaa aacaaagcac taaaatgggc
    30361 atgtgaattt cttataacag aagaagaaat aatacatgtt attaattcac acgctacaag
    30421 tgtatacgaa atagctgaag aattacaagt tagcatcaac ttcttactaa aaagattaga
    30481 atttctatca aaaaagaaaa gcatgttgga cttaggaaat aatagatttt tagtattaac
    30541 taatttgcca aatttctaca tatatgagga tattttt
//
LOCUS       DAMJWP010000020        30244 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.021, whole genome shotgun sequence.
ACCESSION   DAMJWP010000020 DAMJWP010000000
VERSION     DAMJWP010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30244
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.021"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            86..1144
                     /locus_tag="PC700_000850"
     CDS             86..1144
                     /locus_tag="PC700_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HDF2580173.1"
                     /translation="MIVCNNPGTWMRMYPQLRHAVWHGVTLADFAFPFFVISLGVTIP
                     ISINSKLKNNKSTLSIILSIFKRSILLILFGFFLNYLGNPDLNSVRILGVLQRMGLVY
                     FVTSLVYLLLKKLNVGSTATIITFLCIATFIIVGYYILAKPYGFELEGSLAQLVDLHF
                     FKGHLYKPEFEPDGFLTSIVAISSGMLGCTMGCVLLKENIGEYKKFFKILVMSIILLI
                     GAFIFNQYFPFNKRLWSSSFVLLMAGSYGMLLSIFYFICDIKNKSKIFTPIIALGSSP
                     IFTYMCLEILSHVFWNVPKLTNKVDYPTTLVEWTTYELITPWAGTTWDSLIFSLLYVL
                     FWVIVMSIMYKKKIFIKI"
     gene            1398..3422
                     /locus_tag="PC700_000851"
     CDS             1398..3422
                     /locus_tag="PC700_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2580174.1"
                     /translation="MKKTTKLLATGMLSVAMVAPNVALAAENTTANTESNSDININLQ
                     RKSVVLGSKSNASVKFKEKLNADSITLNFMCYDMPLEATLNYNEKTDSYEGVINYNKD
                     PEYLNVWELQSIKINGKDEQKVLNKEDLESMGLNLKDYDVTQEFIISDANSTKAVNEY
                     MRKTSAPVKKLAGATRFETAVEISKQGWKDGSSKVVIVNGELAADGITATPLASTYDA
                     PILLANKDDIPESTKAELKRLNPSDVIIIGDDGSVSQKAVSQIKSAVNVNVTRIGGVD
                     RHETSLLIAKEIDKYHDVNKIYIANGYAGEYDALNISSKAGEDQQPIILANKDSVPQG
                     TYNWLSSQGLEEAYYIGGSQSLSSKIIDQISKIAKNGTSKNRVSGADRHETNANVIKT
                     FYPDKELSAMLVAKSDIIVDSITAGPLAAKLKAPILITPKTYVSAYHSTNLSEKTAGT
                     VYQIGDGMKDSVINSIASSLSKHNAPTEPDNSGSAAGKTVVIDPGHGGSDSGATSGLN
                     GGAQEKKYTLNTALATTEYLRSKGINVVMTRDTDKTMALGERTALSNTIKPDLFTSIH
                     YNASNGAGNGVEIYYKVKDKNGGTTKTAASNILKRILEKFNMKNRGIKTRTLDNGKDY
                     LYVLRNNNYPAILVECAFIDNKSDMDKLNTAEKVKTMGTQIGIGIEDTVK"
     gene            3642..4328
                     /locus_tag="PC700_000852"
     CDS             3642..4328
                     /locus_tag="PC700_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDF2580175.1"
                     /translation="MSGYTNDECEIPKIKSYPGADKEIASEIDYSIVKGTVLFDLYQR
                     IMDLVLSIIGLVIGLPLIAIFGILIKIEDKGPITYKQERLGKCGRRFYIYKLRSMRTD
                     AEKFGAQWAEKDDPRITKVGKFIRKTRIDEIPQLFNILKGDMGLIGPRPERPNFTVQF
                     NEEIPGFINRLAIKPGLTGWAQVNGGYEITPEEKLKEDIYYIKNRSILLDFKILFKTV
                     KVVLTGDGAR"
     gene            4423..5478
                     /locus_tag="PC700_000853"
     CDS             4423..5478
                     /locus_tag="PC700_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp7"
                     /protein_id="HDF2580176.1"
                     /translation="MIKKISTILSLVLLISISSTIEVFADANPKRELIEGSIPEISTE
                     LNKRAFKDSKEVILVNEESIVDSISATPLAYAKNAPIVVTKSKNLGRVTRNYLKELGP
                     EKVTIVGGLKAVSKDAERNIEKMGIKVERIRGKDRYDTSLKIAREMYRTVGFDEAFLL
                     SSTTGLENAISVYSYAAKSGMPIIWAKDEGFEEQIDFLKGKNLKKIYALGDSKEFIAE
                     IDSNLKNIEGIKQINKSSTNVDLIKKFYDEKDIKKIYTAKLDFGSRSDVNEYISLGVV
                     SAKENMPILICSDNLSRAQDKFLKDSNINDVVEVGYTVGDYSLFKSIFNLTFLSCIVL
                     ILLLLLITFRALRYESK"
     gene            5593..7152
                     /gene="murJ"
                     /locus_tag="PC700_000854"
     CDS             5593..7152
                     /gene="murJ"
                     /locus_tag="PC700_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HDF2580177.1"
                     /translation="MSKTAKAALWIMAATMFSKVLGFLRELVLANFYGTGMYADVFVL
                     TLNIPGLIIAVIGSAVATTYIPMYFETKKRLGDEGALKFTNNVLNICYIMAIVIAIIG
                     LLFTEQFVTVFAAGFRNDPAKFQAAILFTKIMISGVLFLSGSKIFSSFLQVNDSFVIP
                     GLIGIPYNIIIIAAIALSAGKNVWILPAGALLAMASQLLFQLPFAFKKSYKYKPYINL
                     KDESIKELVNLVLPMLVGVAVGQLNIFVDRLLATTLGDGKLSALNYANRLNEFVMALF
                     VTSIITVIYPKLAKMSGKDDKEGFISTIVKSSNCIILVVLPISIGAIILAEPLVRILF
                     QRGKFDALSTDLTSIALRLYSLGLLACGVRDVLYRAFYSLSDTKTPMINGSIALIINI
                     VLNLILIRPLGHAGIAISTSTSNIITVILLFISLKKKNGYFGGDKIIKTGLKSLVASG
                     VMAVATLLIYNNLYAFMGSGTIKEIISVGAGVLGGASVYTVLIVLFKVEEMDLAFEFL
                     RKGKQKLLRRR"
     gene            7194..8900
                     /locus_tag="PC700_000855"
     CDS             7194..8900
                     /locus_tag="PC700_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDF2580178.1"
                     /translation="MDYKNNYEMWLNSPYFDEQTKNELLSIKDDEKEIQDRFYKNLEF
                     GTGGLRGIIGAGTNRINIYTVRRATLGVLNYIMKTQGEEGKQKGIVIAHDSRYMSREF
                     CIEVAKTLSAYGVKAYIFEELKPTPELSFAVRYLKCAMGIVITASHNPKEYNGYKVYD
                     SDGGQICIDMANDIIAEVNKIDDYSTIKSIDFEEALSKNLITILDNEVDDEFIKAVKK
                     QVLRQNIIDEYGKKLKIIYTPIHGTGNKPVRKVLNECGFENVMVVKEQELPDSNFSTV
                     KYPNPEEKSVFNIAIEMAKNNDTDLIIGTDPDCDRVGIVVKDSSGEYVVLNGNQVGSL
                     LVRYILESLVEENKLPKNNPTIIKTIVTSELGAKIAKAYNVDCLNTLTGFKFIGEKIK
                     AFEESNDRSFIMGYEESYGYLIGTHARDKDGVVSSLMICEMAAYYSSKGMNLYEALID
                     TYNKFGYYKEDLKSVTLKGIDGIKKIKEMMLYFRSVKIDNVADVKVDKILDYKDGVDD
                     LPKSDVLKFLLEDGSWIAIRPSGTEPKIKFYFGANSDNQEDVEFKLNNLISYILNVVD
                     SI"
     gene            8922..9995
                     /locus_tag="PC700_000856"
     CDS             8922..9995
                     /locus_tag="PC700_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HDF2580179.1"
                     /translation="MKVYNVIMAGGGGTRFWPLSRQEVPKQLINLSGEDALINETINR
                     IDSLAKKDDLFIVTNEKQLEALKDIVKDKCLDSNILPEPCARNTAAAIGFAAFNIMKK
                     YGDGVMCVYPADHYIKDEKEFKSILEKAIYIAENNDKLVTIGITPTFPSTGYGYINFN
                     RENTIEDVAYEVVEFVEKPNYEIAKEYVNSKKYVWNSGMFVWKVSKILEDFKRYLPKV
                     YEKLEYISKYLGTKEEMEKIKEIYPTIQSISIDYGIMERSNDVIVVPGDFGWNDVGSW
                     DSLGAIYPTDDEGNIKRGENITIDTKNSIIYSDDKLISTIGISDLIVVSTNDAVMVCR
                     KDKAQDVKKIVEQLKEEDRQEYM"
     gene            10007..10729
                     /locus_tag="PC700_000857"
     CDS             10007..10729
                     /locus_tag="PC700_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF2580180.1"
                     /translation="MIPKKIHYVWFGGPKGNIENICINSWKEKLPEYEIVEWNEKNFD
                     IEKEIKGNKFLEECYKRKLWAFISDYTRIKVLYEQGGVYMDTDMQILKDITPLLENNR
                     LICGYEDDREYINGAIIGVEKGHPFLKDLLEYYEKEVLTSSLFTIPKIMTHLMEKNYK
                     KIDPNNYEEGIRVYDKEYFYPFGFKEDFTPECITENTFGIHWWGKSWAKKRNYFLESK
                     HLTGVNKIWKCCKIFASNTLRS"
     gene            10745..11902
                     /locus_tag="PC700_000858"
     CDS             10745..11902
                     /locus_tag="PC700_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide polymerase"
                     /protein_id="HDF2580181.1"
                     /translation="MKTLNDDRNIMIRKYLYIFVLFIILLRKYMISFIDPNIDIGMIK
                     SALFYSSIGILMLLFLFDKRKSITEMVLVGVCVLLYLLNREGAILLIVLLAVSAKQID
                     DKFIVKNYLIISACFLMVAILLFNLFPSLIFNQEVHYRYIEKIDMLVTRMDFGLGNPN
                     SVFYHMVTIYAAYIFLRYKDYNKWDRIILFGSAFFVYQTTYSRTGFFTILAGLIFVEI
                     IRWIDIKKIKGLPMLLKTLPIILTLFSVIIGTIFDKSTLLNRLLASRPKFWHVYLAEK
                     GNFLKPFGNSYSPAIKATNPLDSSYVYIISVLGVVACILFMYLMYKGIESFIEKDKKA
                     YLVALFIFLLYSFAENLLLEASFSFAVVLLIKEVILNDKREIDLWKMKSRR"
     gene            11905..12744
                     /locus_tag="PC700_000859"
     CDS             11905..12744
                     /locus_tag="PC700_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2580182.1"
                     /translation="MLISLIMPTLNRYDDIYLLMDSLENQTYKNFELIVVDQNDNSKV
                     KEIVDKYIDKLDIKYIKSSKKGLSYNRNVGIDNAVGQIIGFPDDDCVYENDTLEKVIN
                     FFNKNEDYKIYSCKTMDSNKVDAFKKMYDGTCDITSSNVLDTITSITFFIDFEGKDYT
                     RFDEKLGVGGEFGAGEEIDYVLNLLSLGFKGKYFGNDIIYHPAKKHSKSKEKYQKDYN
                     YGRGFGALCKKEIVYRKNYTFAKVMVSKLVRNIGGLILSSNRDYHSATIKGRINGFRQ
                     YKL"
     gene            12759..13934
                     /locus_tag="PC700_000860"
     CDS             12759..13934
                     /locus_tag="PC700_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDF2580183.1"
                     /translation="MDKVKFLINMLLAFFIYPFNKHKFNGRDIWLIGGHSGDIYNDNS
                     KFFYEYMLKEHNDVETYWVVNKDSKVFDKIPGKKLIRGSVENYLYYYNSKAIVFSHAP
                     SADIAPYNFAVPVLNYFHKKTIKVFLNHGTISFKKRKPMNKKFKNIIDNLYKSYNIVT
                     ASSEFERNVMVNDWGMLDDSVYIIGNARYDNLPTNEVAQTRDILYTPTWRDWIKFSSG
                     KFTDTDYFKNIMNFLNDDKLNKILDEKDINVKIYMHHLMHEFIDDIKENITGKRIVFL
                     DKGVTLANEIRKSAANITDYSSVAIDFLYMNRPILFYQFDLDEYMEKVDSYIDLKSEM
                     FGSLAYNNDEAVNKLIDIIENNFEVMDNQKNERNKFFRYNDNKNCKRIYDCVLSKIK"
     gene            13963..14712
                     /locus_tag="PC700_000861"
     CDS             13963..14712
                     /locus_tag="PC700_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2580184.1"
                     /translation="MKKNLVSIITPMYNSEKFIEATIKSVLNQTYQEWEMLIIDDCST
                     DNSPNIVKSYMQQDSRIKCIKTETNKGVSNARNLALSKATGQFIAFLDSDDQWNSSKL
                     EKQVNFMLENDYVISFTSYELMDENDKKLNKVIKVPPNVDYKRLLKGNILGCLTVVID
                     KSKLDFEIRMSGVRHEDYVLWLSILKKGHIAHGINEVLALYRKSSNSLSGNKIKAAMW
                     TWNIYRNIEKIPLYKAIYYFINYGINGIKKS"
     gene            14734..15642
                     /locus_tag="PC700_000862"
     CDS             14734..15642
                     /locus_tag="PC700_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2580185.1"
                     /translation="MYKYKFTVFTPTYNRAHLLENLYNDLKAQTYDFNDFEWLIVDDG
                     SSDSTKELVEKFISENKLNIRYIYKENGGKHTAINLGVKNADGELFFIVDSDDGLIKD
                     SLEIANDEWDSLENKEGFSGVVGLCVYPSGDLIGTEMPEDKKICHFADLYFKYGVKGD
                     KSIVFVTDELIKFPFPERKEVRFLPESVVWNEMSKYYKVICVNKPMIIRDYLDDGLTK
                     NILSKNALRGRALEFLYLINQNTYPLSRYPYMWIKNYINLARYSLLSDSHYFGELRKV
                     SDKLLYLALFPLGYYKYIGQRKLVSK"
     gene            15690..16442
                     /locus_tag="PC700_000863"
     CDS             15690..16442
                     /locus_tag="PC700_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2580186.1"
                     /translation="MNEPLVSIITPVYNSEEFLSETIKSIQNQTYKNWQLLLVDDCSK
                     DNSSEIIKSFRKEDARIKYIKLEKNSGAAVSRNVGIKNAEGRFIAFVDSDDLWDSRKL
                     EIQIEYMLKENVGFSFTSYRYMRQDGSKTNKVARAPKKIDYEGLLRNTIIGCSTVVID
                     KEIVGEFSMPLVRRGQDTATWLQLLKKEKYAYGIQEDLVNYRLVGNSISSNKIKALKR
                     TWNTYRNVENLSLPKSLYVFCFYVFNAIKKRV"
     gene            16420..17763
                     /locus_tag="PC700_000864"
     CDS             16420..17763
                     /locus_tag="PC700_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HDF2580187.1"
                     /translation="MPLKKEYKFYILGGKKMKIAIAGTGYVGLVTGVCLAEVGNENVV
                     CVDVDEQKVKSMKLGIAPIYEEGLEELMVKNYNRGRIDYTTDYKKAYEDVDVILIAVG
                     TPERSDGSANLDYIKAVAKQIAETVTKDTLVVIKSTVPIGTNEEIEDYINRNLKNNVK
                     VELASNPEFLSQGTAVKDTLYASRIVIGVESEWAREILEEIYKPFNQPIIVTNRNSAE
                     MIKYASNNFLALKISFMNDIANLCEIAGANIEDVALGMSYDDRIGSKFLKAGIGYGGS
                     CFPKDTKALHWLSEHNGYELKTVKAAIEVNESQKLKLVRKASKLVESFQDMKVAVLGL
                     TFKPDTDDIREAPSIPNIKYLLERGARVTVYDPIGVNNTKKIFGDSILYADSVDDAIE
                     DAEICFIMTEWKDVLNYDIHKYKEKMKNPLVLDGRNCYKIKDMEELGIEYYSIGR"
     gene            17769..18860
                     /locus_tag="PC700_000865"
     CDS             17769..18860
                     /locus_tag="PC700_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF2580188.1"
                     /translation="MKTVCYLADGSNPHTLKWCSFFKNKGYDIHVISLNGGHMEGIKI
                     YNFGSNVEELKNENISKKTGYLGSIKQIKKLVNEIKPDILHAQYASSYGFIGSLLGYH
                     PYIISVWGTDIYDFPNNGFIQKQIIKHNFRKADYIFSNSRDMAKEANKYTAKHVDVTF
                     FGVDMDRFKPMEVEKEDAFVIGIIKSLEKKYGIEYLIQAFKMLKDEYKDKKIILKIGG
                     SGSQMDNLINLAKELGIENDVQFLGRISPENVSKTFNSFDVTVFPSLREGFGVAAIES
                     EACEVPVIVTNVGGHPESVWENETGLIVEPKQPEEIKNAIIKLMENDELRLNMGKKGR
                     QFVRENYEVNLNFNDIEKIYDSIFDKYKK"
     gene            18944..20857
                     /locus_tag="PC700_000866"
     CDS             18944..20857
                     /locus_tag="PC700_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDF2580189.1"
                     /translation="MRESIDLEKESFFNRLFKKYKFRHLTLLILDICSVLVAFRMSLS
                     LTGNLNAFTTNDIIISVCIYIVLHIVSFRLFKCYNTLWRYAGEEEIISIFVATLAYLI
                     PIYIINKLLGFDYPIMFYVLNTIFIIMFTSGARIAYRAIRIVMNKTYSRGKVSNILII
                     GAGDAGEMVIQELKRNSELKKVAVAIIDDDKDKIGRIIHNVKIVGTTSDIKAVVEKYN
                     VDEIIFSIANIEKRRKKEIIDICKNTNCKIKTIPGIYEIIDGKVDIKQIREVEIEDLL
                     GREPIKTNLREISNYIEGKVILITGGGGSIGSELCRQIAGFNPKELIIVDNYENNAYS
                     IQQELLRKYKNKLDLKTVIASIREEKRMDEIFNKYKPEVVFHAAAHKHVPLMESSPGE
                     AIKNNIFGTLNIAGLSSKYRAKKFVLISTDKAVNPTNIMGATKRAAEMIIQTMNAESQ
                     TEFVAVRFGNVLGSNGSVIPLFKKQIEDGGPVTVTHPDIIRYFMTIPEAVGLVIQAGA
                     MAKGGEIFVLDMGEPVRILDLAKNLIKFSGFEPDVDIKIEFSGLRPGEKLYEELLMSE
                     EGLLDTEHKKIFIGRPIDVDREKITKYLKLLREITNNEEVEKIDGIMRELVPTYIKPE
                     DANIKEIATTREK"
     gene            21056..21763
                     /locus_tag="PC700_000867"
     CDS             21056..21763
                     /locus_tag="PC700_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF2580190.1"
                     /translation="MMNIKNKKHILKKFIAMVLIAGVVTVEAGAITASAAEPTNSPMS
                     ATVDQCDFLNVRSGASANDAVVGKINTGDKVEVLELHSNGWIKIKSVDNVTGWVNGDY
                     LTIQGGNVDAKVQNVLNLAFKQQGKPYRWGATGPNAFDCSGFTSYVYKNGAGVNLPRV
                     SRSQATVGKKVSRAELKPGDLVFFGSGGSINHVGLYVGDSKFIHSPQTGDVVKVTSMA
                     PGTNYARRLITATRVLQ"
     gene            22065..24176
                     /locus_tag="PC700_000868"
     CDS             22065..24176
                     /locus_tag="PC700_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding glycosyl-hydrolase Cwp19"
                     /protein_id="HDF2580191.1"
                     /translation="MKKISILVLSLIMTLTMCSVSSFADSSNDKEMRAAWISTVYNLD
                     WPKTKNNEAKQKKEYTDLLDKLKSVGINTAVVQVRPKSDALYKSNINPWSEYLTGTQG
                     KDPGYDPLPFLIEEAHKRGMEFHAWFNPYRITMADESIDKLPANHPAKKNPSWVVKHG
                     NKYYYDPGLPEVRKYIVDSIAEVVQNYDIDGVHFDDYFYPGVSFNDTATYQKYGKGQN
                     KDNWRRENVNTLLRDVKASIKSIKPNVVFGVSPAGIWRNKSSDPTGSDTSGNESYVGT
                     YADTRAWIKQGLIDYVVPQLYWPIGLKAADYSKLVAWWANEVKGTNVDLYIGQGIYKQ
                     GQSSYGGQNIAKEIVQQVTLNRKYSEIKGSMYFSAKDIANSTSIQKDLKSLYSSSEEP
                     VTPPSNVKVEKLRGDERYDTAVAISKKGWATNSDTVVLVNGYSIVDGITSTPLATSND
                     APILLVNKDNIPTSTKNELKRLNPSKVILIGGNNSIGDKVESEIKDTLSNVSINRVGG
                     SDRYSTSLMIAKELVKTNPVEKLYITSGTGEADSLSIASKAGEEKQPIVLVSKDNVSD
                     EVYNWISDLKVKDAYFIGGNLSISDSVINKLDKVITNDVSKNRIAGENREETNGKVIQ
                     KFYPNAEYSSMFVSKSNQLVDALTSGPLAAKLKSPVVMLGNSVTSAQKTALEPKKTAL
                     VYEAGDGINQNTLNTFLNLVK"
     gene            complement(24374..25339)
                     /locus_tag="PC700_000869"
     CDS             complement(24374..25339)
                     /locus_tag="PC700_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDF2580192.1"
                     /translation="MSKLKKFVILLAFLVVIFPISVYGYFYYKLSAIHDSSISSDLLD
                     NNDHKNEDGIINILLMGTDARPNEDSSRSDAMMILTIDNKHNDIKLTSLARDSYVDIP
                     GHGKQKLTHAYAYGQADLLIQTIEENFNIDIQNYACVNFESFMYIIDAIGGVEVTVEK
                     GEIRELNKFIPETYKWNKSDDKGSIQYIRNSGKQTLNGYQALSFARIRHNDTAFARDG
                     RQRQIIQAIIKKTETLPVTKYPGLLDAVLPYVKTNMKPNAILSLGAQVLKMGDLNIKQ
                     FEFPIDDEIHSTGGIYGKAGWVLRFDPDTLDILHDFIFNDIEFKQ"
     gene            complement(25624..26580)
                     /locus_tag="PC700_000870"
     CDS             complement(25624..26580)
                     /locus_tag="PC700_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDF2580193.1"
                     /translation="MSGLKKFVIFLALLVIAFPVSIYGYVFTKLKTIQDDSINTEALS
                     SNDYKNEENITNILLLGTDARPGEEVSRSDAMMILTIDNKNKSLKLTSLNRDTYVDIP
                     GRGEQKLTHAYVYGQANLLIETIEENFQLDIQNYAVVDFFSFMDIVDALGGVTVTVDK
                     SEIRELNKFIPETYKWNKNDNKGSLKYIRNSGEQKLNGYQTLAFSRIRKNDSTDERDR
                     RQRSVIQSLINGVKDLPVTKYPNLVNTILPYVKTNMNPNEIISLGKELLSIGNLNLKS
                     MEFPLSTENGRKIGNAGYVIPFEEYELDAMHDFIFKDIMVED"
     gene            26948..27736
                     /locus_tag="PC700_000871"
     CDS             26948..27736
                     /locus_tag="PC700_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2580194.1"
                     /translation="MIKLIATDLDGTLLDEKSEINPEFYKVFKKLRERGIMFSAASGR
                     QYQNLIKKFEDIKDDMMFISENGTLVVYKGKEILSNPLNKELVNEIIETTRSIKGKKI
                     VMSGKKYAYIESKDEAFIQEVSTYYAKFKIVDDLTKVEGDILKIAVFDFKGAEHNNNI
                     YFEKFSDRAQVCISGVEWLDLTAKGANKGSAIKKVQKMLDIKYEETMVFGDQLNDVEM
                     MKSAYHSYAMENANEHLKQIARFRAKRNTENGVVDKIKEVIKIG"
     gene            complement(27859..28329)
                     /locus_tag="PC700_000872"
     CDS             complement(27859..28329)
                     /locus_tag="PC700_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HDF2580195.1"
                     /translation="MKKILILFFSIILSLGMVSCSSSGDYKNVSTSKIKKALKSSDLL
                     IKESKIFDAKDFNYFNDVEEDIIQGFVINSSEKLSLEDIIVIKTDEVDKIYKTLENYK
                     KDMIQAPFGEGHGEKINSEIANNTIIEKAGRYVYLISAENATQIENKILNIIKK"
     gene            28468..29112
                     /gene="uppS"
                     /locus_tag="PC700_000873"
     CDS             28468..29112
                     /gene="uppS"
                     /locus_tag="PC700_000873"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HDF2580196.1"
                     /translation="MRIPKHVGIIPDGNRRWASQNGMTKDKGYESGIDPGLESYRLCR
                     DLGCEEVTFYGFTTDNTRRPSVQTKAFTKACIDAANILIDEGAALLVVGNTKSPMFPE
                     ELLPYTNRIKAKEGQIKANLLVNYGWYWDLNNISGVENVNKNNIQDYIQSFDVSRLDL
                     IIRWGGRRRLSGFLPVQSIYADFYVIDNYWPDFKSDDIKDAFSWYDTQDVTLGG"
     gene            29155..30060
                     /locus_tag="PC700_000874"
     CDS             29155..30060
                     /locus_tag="PC700_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2580197.1"
                     /translation="MKKKLLDGKITLIICKSVKIYTKKGEEMSKGNNNNNSRNKSKKT
                     SHLNRKKRKLNKKKLAVLICFTVLFLFIAFKATQGVVALVKSMDKSNKTSQQQKVNSE
                     QFDLGNEEENKKKKYTVFIDPGHGGNDKGTESKTSNRYEKDLNLQIAKKLANKLSKQK
                     DIQVVVSRTDDTYISLKDRAILANNSSADVLVSIHLNAEKNGNTATGIETWYRNKATD
                     GSKELAQTVQSTIVSYVKVRDRGIVENNFEVLRESNMPAILIECGFLTTPSEEQKIIN
                     EKYQDQLAEGIVQGVLSYLDSKGNK"
BASE COUNT        11990 a         3299 c         5371 g         9584 t
ORIGIN      
        1 aatttcaaat aatgttgtag attctaaact tacgaatagt aggattaaat caatagatat
       61 aataagaggg ctatcaatag ccctcatgat agtctgcaat aatcctggga cttggatgag
      121 aatgtatcct caattaagac atgctgtatg gcatggtgta acacttgcgg attttgcatt
      181 tccttttttt gttatatctt taggtgttac aattccaata tcaataaata gtaaactaaa
      241 aaataataaa tccacactga gtataatact tagtatattc aagagaagta tcttattgat
      301 attatttgga tttttcttaa attacctagg aaatcctgat ttaaattctg tgagaatact
      361 tggagtactt caacgtatgg gattagtcta ttttgtgact agtttagtat atttactact
      421 aaaaaaatta aatgttggaa gtactgctac aataattact tttctatgta tagctacatt
      481 tataatagtt gggtactaca tattagcaaa accttatgga tttgagctag aaggttctct
      541 tgcacagtta gtggatttac atttcttcaa gggacatttg tacaagccag aatttgaacc
      601 agatggtttt ttaactagta tagtagcaat ttcttcaggt atgttgggat gcactatggg
      661 atgtgtactt ttaaaagaaa acataggaga atataaaaaa ttctttaaaa tactggttat
      721 gagcataatc ctgctaatag gtgcttttat ctttaatcag tattttccat ttaataaaag
      781 attatggtct agctcatttg tacttttaat ggctggcagt tatggtatgt tactgtcaat
      841 attttatttt atatgtgaca tcaaaaataa aagtaaaata tttaccccaa taatagcact
      901 tggaagtagc cctattttta catatatgtg ccttgagata ttgagtcatg ttttttggaa
      961 tgttccaaaa cttacgaaca aagtcgatta tccaactacc ttggtagagt ggactacata
     1021 tgaacttata acaccttggg caggtacaac ttgggattct cttatatttt ctttattgta
     1081 tgtcctattc tgggttatag taatgtctat aatgtacaag aaaaaaatat ttattaaaat
     1141 ttagtgcaat atttttagat gataaagtct ttgatttgtt caaatatacg gaaactatca
     1201 aatataatca aaatttctcc tcgataaaat ttaaaaacca tgccataaaa gattacaaaa
     1261 agtttacaat ttgtaatttt ttgtgctata atgaaaaaag atttacataa tattgaattt
     1321 atgtaatata aaacaagctt aagatgtatt aaaaaagatt taaagattta aagaaccaaa
     1381 aaattagggg ggatatgatg aaaaaaacaa caaaattatt ggcaacagga atgttgtctg
     1441 tagcaatggt ggcaccaaac gtagctttag cggctgaaaa tactactgca aatacagaat
     1501 ctaattcaga tattaatata aatttacaaa gaaaaagtgt agtgttagga agtaaatcta
     1561 atgcaagtgt aaaatttaaa gaaaaattaa atgctgattc aataacatta aattttatgt
     1621 gttatgatat gccacttgaa gctactttaa attacaatga aaagacagat tcatatgaag
     1681 gtgtaataaa ctataacaaa gacccagaat acttaaatgt atgggaactt caaagcataa
     1741 aaataaatgg taaagatgaa cagaaagttt taaacaaaga agacttagag tctatgggat
     1801 taaatcttaa ggattatgat gttacacaag agtttataat aagtgatgca aattcaacaa
     1861 aagcagtaaa tgaatatatg agaaaaactt ctgcgccagt aaaaaaactt gctggagcta
     1921 ctagatttga aactgctgtt gagataagta aacaaggttg gaaagatggg tctagtaagg
     1981 ttgtaatagt taatggagaa ttagcagcag atggaattac agctactcca cttgcatcta
     2041 catatgatgc accaatatta cttgcaaata aagatgatat accagaaagt acaaaagcag
     2101 aattaaaacg tctaaatcca agtgatgtaa taattatagg tgatgatggt tcagtttcac
     2161 aaaaggcagt atctcaaatt aaatctgctg taaatgtaaa tgtaactcgt attggtggag
     2221 ttgatagaca tgaaacatct ttacttatag caaaagaaat agataagtat catgatgtta
     2281 ataaaatata catagcaaat ggatatgctg gtgaatatga tgctcttaat atttcatcaa
     2341 aagctggtga agaccaacaa cctataatat tagctaataa agactctgta ccacaaggta
     2401 catataattg gttatcttca caaggtttag aagaagctta ttacataggt ggtagtcaaa
     2461 gtttatcatc aaaaattata gaccaaatat ctaaaatagc aaaaaatggt acttcaaaaa
     2521 atagagtttc tggagcagat agacatgaaa caaatgcaaa tgtaattaag actttctatc
     2581 cagataaaga gttgagtgca atgttagttg caaaatctga cataatagtt gactcaataa
     2641 cagcaggacc tttagcagct aaattaaaag ctccaatact tataactcca aagacttatg
     2701 tttctgcata ccatagcaca aacttatcag aaaaaacagc aggaacagta tatcaaattg
     2761 gtgatgggat gaaagatagt gttataaatt ctatagcatc tagtttatca aaacacaatg
     2821 caccaacaga accagataat tcgggttcag cagcaggaaa aactgtagtg atagacccag
     2881 gtcatggggg ttctgattca ggggcaacaa gtggattaaa tggtggagca caagaaaaga
     2941 aatatactct taatacagca ttagcaacta ctgaatattt acgttcaaaa ggtataaatg
     3001 ttgttatgac tagagataca gacaaaacaa tggctttagg agaaagaaca gcattatcaa
     3061 acactataaa gccagattta tttacaagta tacattacaa tgcgtctaat ggagctggta
     3121 atggtgtgga aatttattac aaagtaaaag ataaaaatgg tggaacaact aagactgcag
     3181 catcaaatat cttaaaaaga atactagaaa aatttaacat gaaaaataga ggaatcaaga
     3241 caagaacact tgataatgga aaagattacc tatatgtgtt aagaaataac aattatccag
     3301 caatacttgt tgaatgtgca tttattgaca ataagagcga tatggataag ttaaatactg
     3361 cagaaaaagt aaaaacaatg ggaactcaaa ttggaatagg aatagaagat acagtaaaat
     3421 aaaataaaaa atattaaagc aaaacatatc atctcctgtt ataatgtaaa atataacaag
     3481 agatgatata tttttgtaaa gaatcaaatt gtttacaatg atgtttcttg tattgataat
     3541 ctgctcatta ttgagtataa tatagaaata agtcaaaaat aagatagaaa taagttgaaa
     3601 ttaagaatta aaaaaatttt tattccaata tggaggtaac tatgtctgga tatactaatg
     3661 atgagtgtga aatacctaaa attaaaagct atccaggggc agataaagaa attgcaagtg
     3721 agattgacta tagcatagta aaaggaacgg ttttattcga cttgtaccaa agaattatgg
     3781 atttagtttt atctataata ggacttgtga taggtttacc tttaatagca atatttggaa
     3841 ttcttattaa aatagaggac aaaggaccaa taacttataa gcaagaaaga ctaggaaaat
     3901 gcgggagaag attctatata tataaattaa gatctatgag aacagacgca gaaaaatttg
     3961 gcgcacaatg ggctgaaaaa gatgatccta gaataactaa ggttggaaag tttattagga
     4021 aaactagaat tgatgagatt ccacagctgt ttaatatatt aaagggggat atgggcttaa
     4081 taggccctag accagagaga ccgaacttta ctgttcaatt caatgaggaa atacctggat
     4141 ttatcaacag gctcgctata aaacctgggc ttacaggttg ggcacaagta aatggtggat
     4201 acgaaatcac tcctgaagaa aaacttaaag aagatattta ctatataaaa aataggagta
     4261 tattacttga ctttaagata ctctttaaaa ctgttaaagt agtattgaca ggagacggag
     4321 ctagataatt ttaaaatatt agattactaa tcaatgaaaa agctctttaa aaagagcttt
     4381 tttattgtca aaaaatacaa aaaagaaaag gaatgagaac ttatgattaa aaaaatttct
     4441 actatattat cgttagtttt gttaatctct atttctagca caatagaggt atttgcagat
     4501 gcaaatccca aaagagaatt gatagaaggg agtataccag agatttcgac agaattaaat
     4561 aaaagagcat ttaaggattc taaagaagta attcttgtaa atgaagagtc aattgtagat
     4621 agtataagtg caacaccttt agcatatgca aaaaatgctc caatagttgt aactaagagt
     4681 aaaaatcttg gtagagtaac aagaaattat ctaaaagaac taggtccaga gaaggttacg
     4741 atagtaggtg gtttaaaggc agtatctaaa gatgctgaac gtaatataga aaaaatggga
     4801 ataaaagtag aaagaataag aggaaaagac agatatgata catctttaaa gatagctaga
     4861 gaaatgtata gaacagtagg atttgatgaa gcatttttac ttagctcaac aacaggatta
     4921 gagaatgcca tatctgtata ttcttatgcc gctaaaagtg gaatgccaat aatatgggct
     4981 aaagatgaag ggtttgagga acaaattgat ttcttaaaag gtaagaatct taaaaaaata
     5041 tatgcattag gtgattcaaa agaatttatt gcagagattg attctaactt gaaaaatata
     5101 gaaggaataa agcaaataaa taagtcaagt acaaatgttg atttaataaa gaagttttat
     5161 gatgaaaaag atataaagaa aatctatact gctaaattag actttggtag cagatctgat
     5221 gtaaacgaat atatttcttt gggagtagta tcagcaaaag aaaatatgcc aatactgatt
     5281 tgtagtgata acttaagtcg tgcacaagat aaattcttga aagatagcaa tataaatgat
     5341 gtagttgaag ttgggtatac agtaggtgat tatagtctat ttaagagcat atttaatcta
     5401 acattcttat cttgtatagt attaatacta ttattactat tgattacatt tagggcactt
     5461 agatatgagt cgaaatagtt tttaatttat aaggaagctt ttcataggtt aaatttactt
     5521 aattagaata atgtttataa gcagttttaa aagttctact tttagagttt aaattagata
     5581 aggagaaaga agatgtcaaa aacagctaaa gcagcattgt ggattatggc agccactatg
     5641 ttttcaaaag tattaggttt ccttagagaa cttgtacttg caaatttcta tgggacaggt
     5701 atgtatgcag acgtatttgt attaacatta aatatacctg gtttaatcat agcagtcata
     5761 ggctctgcag ttgctacaac ttatatacct atgtactttg agacaaagaa aagactcgga
     5821 gatgaaggtg ccttaaaatt tactaataat gttttaaata tatgctatat aatggctata
     5881 gtaatagcta ttattgggct tttatttaca gagcaatttg ttacagtatt tgcagcagga
     5941 tttagaaacg accctgctaa gttccaagca gcaatattat ttactaaaat aatgatttca
     6001 ggagttttat tcctaagtgg aagtaagata tttagttcgt ttttacaggt gaatgacagt
     6061 tttgtaattc ctgggcttat aggaatccca tataatatta taataatagc agcaatagca
     6121 ctaagtgcag gtaaaaacgt atggatacta ccagcaggag cactattggc tatggcgagt
     6181 caactattat ttcaactgcc atttgcattt aagaagtcct acaagtataa gccatatata
     6241 aacttaaagg atgagtctat aaaagaatta gtaaacctag tattgcctat gttggtagga
     6301 gtagctgttg gacagttaaa tatatttgtt gacagattat tggcaactac tttgggtgat
     6361 ggaaagttgt ctgctcttaa ctatgcaaat aggctaaatg agtttgttat ggcattattt
     6421 gttacatcta taataacagt tatatatcct aagctagcaa aaatgtctgg taaagatgat
     6481 aaagaaggat ttataagtac tattgtaaaa tcttcaaatt gtattatatt ggtggtgctt
     6541 cctatatcta taggagctat tatattagca gagcctcttg ttaggatatt atttcaaagg
     6601 ggtaaatttg atgcgttatc aacagatctt acatctatag cgcttagatt atattcacta
     6661 ggtttattgg cttgtggagt aagagatgtc ttatataggg cattttactc tctttcagat
     6721 acaaaaacgc caatgataaa tggaagtata gctttaataa taaatatagt tcttaattta
     6781 atattgataa gaccattagg tcatgcaggt attgctatat caactagtac atccaatata
     6841 attacagtta tattgttatt tatatctctt aaaaagaaaa atggatattt tggaggggat
     6901 aaaattataa agactggtct aaaaagtttg gtagcatctg gtgtgatggc agtagctact
     6961 ttattgatat ataataatct atatgcattt atgggttcag gaacaattaa agaaattata
     7021 tcagttggag ctggagtatt aggtggagct tctgtatata ctgtattgat agttttattt
     7081 aaggtagaag agatggactt agcttttgaa tttttaagaa aaggaaaaca aaaactactt
     7141 agaagaagat aattaccttg aaattttaaa ttagagtcag aaaggatgaa aatatggatt
     7201 ataagaataa ttatgagatg tggttaaatt ctccttattt tgatgagcaa acaaaaaatg
     7261 agctattaag cataaaagat gatgaaaaag aaatacaaga tagattctat aagaatttag
     7321 agtttggaac aggtggttta agaggtataa taggagcggg aactaataga atcaatattt
     7381 atactgttag acgtgcgaca ctgggcgttt taaattatat catgaagact caaggagaag
     7441 aaggtaaaca aaagggaata gttatagcac atgatagtag atacatgtct agggagtttt
     7501 gtatagaagt agcaaagact ttaagtgctt atggtgtaaa ggcttatatt tttgaagaat
     7561 taaagcctac accagaactt tcttttgctg ttagatattt aaagtgtgct atgggaatag
     7621 taataacagc aagtcataat cctaaagaat acaatggata taaagtatat gactcagatg
     7681 gtggtcaaat ctgtatagat atggcaaatg atataatagc agaagttaat aagattgatg
     7741 attacagcac tattaaaagt atagattttg aagaagcttt atctaaaaat ttaataacta
     7801 tattagataa tgaagtagat gatgagttta taaaagctgt taagaagcaa gttttaagac
     7861 aaaatataat agatgaatac gggaaaaaat taaaaataat atatacacct attcatggta
     7921 caggaaataa accagtaaga aaagtcttaa atgaatgtgg ttttgaaaat gtaatggtag
     7981 ttaaggagca agaattacca gattctaatt tttcaacagt aaagtatcct aatcctgaag
     8041 aaaagtcagt gtttaatata gctattgaaa tggcaaagaa taatgacact gatttgataa
     8101 taggaacaga ccctgattgt gatagagtag gtatcgttgt aaaagattca agtggtgaat
     8161 atgtagtctt aaatggaaat caagtcggtt cacttttagt tagatatata ttagagagtt
     8221 tagttgaaga aaataaactt cctaaaaaca atcctacaat aataaaaact atagttacat
     8281 cagaactagg tgcaaaaata gcaaaggctt ataatgtaga ttgtttaaat actttaacag
     8341 gatttaaatt tataggagaa aaaataaaag cgtttgaaga aagcaatgat agaagtttta
     8401 taatgggata tgaggaaagc tatggttact taattggaac tcatgctaga gataaagatg
     8461 gtgttgtatc ttcacttatg atatgtgaaa tggcagcata ctattcttca aaaggtatga
     8521 acttgtatga agctttgata gatacatata ataaatttgg atactacaaa gaggacttaa
     8581 agtcagtaac tttaaaaggt atagatggaa taaaaaaaat aaaagagatg atgctttact
     8641 ttagaagtgt taaaatagat aatgtggctg atgtaaaagt agataaaatt ttagactata
     8701 aagatggtgt tgatgattta cctaagtcag atgtacttaa atttttactt gaagatggct
     8761 cttggatagc tataagacct tcaggtacag aacctaaaat taaattttac tttggagcaa
     8821 attcagacaa tcaagaagat gtagaattta agttgaacaa tttaatatct tatatattaa
     8881 atgtagtgga ttctatataa aaagctaagg ggagataaat aatgaaagta tacaatgtca
     8941 taatggctgg aggaggagga actcgtttct ggccacttag tagacaagaa gtacctaaac
     9001 agcttataaa tttaagtgga gaagatgctt taataaacga aactataaac agaatagatt
     9061 ctctagcaaa aaaagatgat ttatttatag taaccaatga gaagcaattg gaagctttaa
     9121 aggatatagt aaaagataaa tgtttggata gtaatatatt gccagagcca tgtgccagaa
     9181 atacagcagc agctataggc tttgcagcat ttaacataat gaaaaaatat ggtgatggtg
     9241 ttatgtgtgt atatccagct gaccattata taaaagatga aaaagaattt aaatctattt
     9301 tggaaaaagc tatctatata gcagaaaata atgataaact agtaactatt gggataactc
     9361 ctacatttcc atcaacagga tatggataca taaattttaa tagagaaaat acgatagaag
     9421 atgttgcata tgaggtagtg gagtttgtag aaaaaccaaa ctatgaaatt gctaaagaat
     9481 atgtaaactc taaaaagtat gtatggaata gtggaatgtt cgtatggaag gtatctaaaa
     9541 tattagaaga ttttaagaga tacttgccaa aagtatatga aaaattagaa tatataagta
     9601 aatacttagg aacaaaagaa gaaatggaaa aaataaaaga aatataccca actatacaat
     9661 caatctctat agattatggt attatggaaa gaagtaatga tgttatagta gttcctggtg
     9721 attttggatg gaatgatgtt ggttcttggg attctctagg tgcaatatat ccaactgatg
     9781 atgaaggtaa catcaaaaga ggtgaaaata ttactataga tactaaaaat tcaattatat
     9841 attcagatga caaattaata tcaacaatag gtattagtga tttgatagta gtatctacga
     9901 atgatgcagt tatggtttgt agaaaagata aagctcaaga tgtcaaaaaa atagtagaac
     9961 agttaaaaga agaagataga caagaatata tgtaggaggt gttcatttga ttcctaaaaa
    10021 aatacattat gtatggtttg gtggtccaaa aggaaatata gaaaatatat gtataaattc
    10081 ttggaaagaa aagcttccag aatatgagat agtagagtgg aatgaaaaaa actttgatat
    10141 agagaaagaa ataaaaggaa ataagttttt ggaagaatgt tataaaagaa aactttgggc
    10201 attcatatct gattatacaa gaataaaagt actctatgaa caaggtggag tttatatgga
    10261 tacagatatg caaatattaa aggatataac tcctctttta gaaaataata ggctaatctg
    10321 tgggtatgaa gatgatagag agtatataaa tggtgctata ataggtgtag aaaaaggaca
    10381 tcctttttta aaagacttat tggagtatta tgaaaaagaa gtacttactt cttcattgtt
    10441 tacaatacca aaaataatga ctcatcttat ggaaaagaat tataagaaga ttgacccaaa
    10501 taattatgaa gaaggaatac gtgtttacga caaagaatat ttttatcctt ttggatttaa
    10561 agaagacttt acaccagaat gtataacaga aaacactttt gggatacatt ggtggggaaa
    10621 aagttgggct aaaaagagaa attatttctt agaaagcaaa cacttaacag gtgtaaataa
    10681 gatatggaag tgttgtaaaa tatttgcaag taatacttta cgaagctaaa agggagaaag
    10741 taaaatgaag actttaaatg atgatagaaa tattatgatt agaaaatatc tttatatatt
    10801 tgttttgttt atcatattac ttagaaagta tatgatatca tttattgacc caaatataga
    10861 tatagggatg attaaatcag cgttatttta ttcttcaata ggaatactaa tgctactttt
    10921 tttatttgat aagagaaaaa gtataacaga aatggtacta gttggagttt gtgtcttact
    10981 ttatttatta aatagggaag gagctatatt attaatcgtt ttattagctg tatcagctaa
    11041 gcaaatagat gataaattta tagttaaaaa ttatttaata atatcggcat gttttttaat
    11101 ggtagctata ttattattta atttatttcc ttcattaata tttaatcagg aagtccacta
    11161 tagatatata gaaaaaatag atatgcttgt tacaagaatg gattttggac ttggtaatcc
    11221 aaatagtgta ttttatcata tggttactat atatgcagca tatatattct taagatataa
    11281 agattataat aaatgggata gaataatttt atttggttct gcattttttg tttatcaaac
    11341 cacatatagt agaactggat ttttcactat attagcagga ttaatatttg tagaaataat
    11401 aagatggata gatataaaaa aaataaaagg actgccaatg ctcctaaaga cattaccaat
    11461 aatacttact ctatttagtg taattattgg tacaatattt gataaaagca ctttattaaa
    11521 tagattatta gcaagtagac ctaagttctg gcatgtatac ttggctgaaa aggggaattt
    11581 tctaaaacct tttggaaatt catatagtcc tgctataaaa gcaactaatc cattagatag
    11641 ttcttatgta tatataatta gtgtactagg agtagttgct tgtatattat ttatgtattt
    11701 gatgtataaa ggaatagaaa gctttataga aaaagataaa aaagcatatt tagtagcttt
    11761 atttatattt ttactatact cttttgcaga aaatttatta ttagaagctt catttagttt
    11821 tgcagtagta ttactaataa aagaagtgat attaaatgat aaaagggaaa tagatttgtg
    11881 gaagatgaaa agtaggaggt agtaatgtta atatcattaa taatgccaac attaaataga
    11941 tatgatgata tatacttact tatggatagc ttagaaaatc aaacttacaa aaactttgaa
    12001 cttatagtag tagaccaaaa tgataatagc aaagttaaag aaatagttga taaatacatt
    12061 gataagttag atataaagta tataaaaagc tctaaaaaag gattgagtta taacagaaat
    12121 gtaggcatag ataatgctgt agggcaaatt ataggtttcc ctgatgatga ttgtgtctac
    12181 gaaaatgata cattagaaaa agttataaat ttctttaata aaaatgaaga ttataaaata
    12241 tacagttgca aaactatgga ttcaaataaa gttgatgcat ttaagaaaat gtatgatgga
    12301 acttgtgata tcacaagttc caacgttttg gatactataa catccataac tttttttatt
    12361 gattttgaag gtaaagatta tacaagattt gatgaaaaat tgggtgttgg tggagagttt
    12421 ggtgctggtg aagagataga ttatgtttta aacttattga gtttaggttt taaaggaaaa
    12481 tactttggaa atgatataat ttaccatcct gcaaaaaaac attctaaatc taaagaaaag
    12541 tatcaaaaag attataatta tggaagaggc tttggagcgc tttgcaaaaa ggaaatagtc
    12601 tatagaaaaa attatacgtt tgcaaaagtt atggtatcaa aacttgtaag aaatataggg
    12661 ggtcttatat tgagttccaa tagagattat catagtgcca ctataaaagg aagaattaat
    12721 ggatttagac agtacaagtt ataatgagga tggtgtttat ggacaaggtt aaatttttaa
    12781 taaatatgct tttggctttt tttatatatc catttaataa gcataagttt aatggtagag
    12841 atatctggct tataggtgga cattcagggg atatatacaa tgataattca aagttttttt
    12901 atgagtatat gctaaaagaa cataatgatg tagaaactta ttgggtagta aataaagaca
    12961 gtaaagtatt tgataaaatt ccaggcaaaa aattaataag aggaagtgta gaaaactatc
    13021 tgtattatta caattcaaaa gctatagtat tttcacatgc accatcagca gatatagcac
    13081 catacaattt tgctgtacct gttcttaatt attttcataa aaaaacgata aaagtgtttt
    13141 taaatcatgg gactataagc tttaaaaaaa gaaaacctat gaacaaaaag ttcaaaaata
    13201 ttatagataa cctatataaa agctataata tagttacagc aagttctgaa tttgaaagaa
    13261 atgttatggt aaatgattgg ggaatgttag atgactcagt ttatataatt gggaatgcaa
    13321 gatatgacaa tttaccaaca aatgaagttg cacaaactcg agatatatta tatactccta
    13381 catggagaga ttggataaaa tttagttcag gaaaatttac ggatacagat tattttaaaa
    13441 atataatgaa ttttttaaat gatgacaaat taaataaaat attagatgag aaagatataa
    13501 atgtaaaaat ttatatgcat catcttatgc atgagtttat agatgatata aaagaaaata
    13561 taacaggaaa acgaattgtg tttttagata aaggggtaac tctagcaaat gaaatcagaa
    13621 aatcagctgc aaatataact gattattcaa gtgtagccat agactttttg tatatgaata
    13681 gacctatctt gttttaccaa ttcgatttgg atgaatatat ggaaaaagtt gattcatata
    13741 tagatttaaa aagtgaaatg tttgggtctt tggcttataa taatgatgaa gccgtgaata
    13801 aacttattga tattattgaa aataattttg aggttatgga taaccaaaaa aatgaaagaa
    13861 ataagttttt cagatataat gataataaga actgcaagag gatatatgat tgtgtattaa
    13921 gtaaaattaa ataaatattt tataaatgat gaggaaggta atatgaagaa gaatttagta
    13981 tctataatta ctcccatgta taattctgaa aaatttattg aagcaaccat aaaatcagta
    14041 ttaaaccaaa cttatcaaga atgggaaatg ttaattattg atgattgctc aacagataat
    14101 agtcctaata tagtcaaatc ttatatgcaa caggatagta gaataaaatg tataaagact
    14161 gagactaata agggtgtctc taatgctaga aatttagcac taagtaaggc aacaggacaa
    14221 tttatagctt ttttagatag tgatgaccaa tggaatagca gtaagttaga aaaacaagta
    14281 aattttatgt tagaaaatga ctatgtaatt tcatttactt catatgaact gatggatgaa
    14341 aatgataaaa aattaaacaa agtaataaaa gttccaccta atgtagatta taaaagatta
    14401 ttaaaaggaa atatcttagg atgtcttact gttgtgattg ataagtctaa attggatttt
    14461 gaaataagaa tgagtggtgt aagacatgaa gactatgttt tatggttatc gatattgaaa
    14521 aaagggcata ttgctcatgg aataaatgaa gttctagcat tatatagaaa atctagcaat
    14581 tccctaagtg gtaataaaat aaaagctgct atgtggactt ggaatatata tagaaatata
    14641 gaaaaaatac ctttatacaa agctatatac tactttatta attatggaat aaacggaatc
    14701 aaaaaaagtt agacagagag gaagatttaa ttaatgtata aatataaatt tactgtattt
    14761 actccgacgt ataacagagc tcatttactt gaaaatttgt acaatgattt aaaagctcaa
    14821 acttatgatt tcaatgattt tgaatggttg attgttgatg atggaagtag cgacagtact
    14881 aaagagttag ttgaaaaatt tatatcagaa aataaattaa atataagata tatatacaaa
    14941 gaaaatggag gaaaacatac tgcaataaat ctaggtgtaa aaaatgcaga tggagagcta
    15001 tttttcattg tagatagtga tgatggttta attaaagatt ctcttgaaat tgctaatgat
    15061 gagtgggatt ctttagagaa taaagaagga tttagtggag ttgttggact ttgtgtatat
    15121 ccaagtggag atttgatagg gacagaaatg cctgaagata agaaaatatg tcactttgca
    15181 gacctatatt ttaagtatgg cgttaaggga gataaaagca tagtatttgt aactgatgag
    15241 cttataaaat ttccttttcc agaaagaaaa gaagtgagat ttttaccaga aagtgtagta
    15301 tggaatgaaa tgtctaaata ctataaagtt atatgtgtaa ataaacctat gataataaga
    15361 gattatttgg atgatggttt gactaagaat atattaagta aaaatgcatt aagaggtaga
    15421 gcattagagt ttttatattt aataaatcaa aatacatatc cattaagtag atatccatat
    15481 atgtggataa aaaattacat aaatttagcc agatactcat tgttgtctga tagtcattat
    15541 tttggtgaat taagaaaagt atctgataaa ttgttgtatt tagcattatt cccattagga
    15601 tattacaaat atattggaca aagaaaatta gtatctaagt aaacttgttg gtatagtact
    15661 gctagttgct atataaggag agtaaaaata tgaatgagcc tttagtatcg ataattactc
    15721 ctgtttataa ttcagaagag tttttatcag agacgataaa gtcaatccaa aatcaaactt
    15781 ataaaaattg gcagctttta ttagtggatg attgttctaa ggacaattca agtgaaatta
    15841 taaaaagttt tcgaaaagaa gatgctagaa taaaatatat aaagcttgaa aaaaattctg
    15901 gagctgcagt atctagaaat gttggtatta agaatgcgga aggtcgattt attgcatttg
    15961 tagatagtga tgatttatgg gatagtagaa aattagagat tcagatagaa tatatgttaa
    16021 aagaaaatgt aggtttttct tttacatctt atagatatat gagacaagat gggtcaaaaa
    16081 cgaataaggt tgcaagagct cctaagaaaa ttgactatga aggattactt agaaatacta
    16141 taataggatg ttctacagtg gtcattgata aggagattgt aggagagttc agcatgcctt
    16201 tggtaagaag aggacaagat actgcgacct ggttacagtt acttaaaaag gaaaaatatg
    16261 catatgggat tcaggaggat ttagtgaatt acagattagt tggaaattct atatcaagta
    16321 acaagataaa agcattaaaa cgaacttgga atacttatag aaatgttgaa aatttaagtt
    16381 taccaaaaag tctatatgta ttttgtttct atgtttttaa tgccattaaa aaaagagtat
    16441 aagttttata tacttggagg taaaaaaatg aaaatagcta tagctgggac gggatatgta
    16501 ggtttggtta caggtgtttg tctagcagag gtaggcaatg aaaatgtagt ctgtgttgat
    16561 gtagatgaac aaaaagttaa atcaatgaaa cttggaatag cacctatata tgaagaaggc
    16621 ctagaagaac ttatggttaa aaattacaac agaggaagaa tagattacac tacagactat
    16681 aaaaaagctt atgaagatgt agatgtaata ttaatagctg taggtacacc tgagagaagt
    16741 gatggctcag ccaacctaga ttatataaag gcagttgcaa agcaaatagc tgaaactgtg
    16801 acaaaagata ctctagtagt aataaaatct acagttccaa ttggtacaaa tgaagagata
    16861 gaagattata taaatagaaa cctaaaaaat aatgtaaaag tagagcttgc atctaatcca
    16921 gagtttttat cacaaggaac agcagtaaaa gatactctat atgcttcaag aatcgttata
    16981 ggtgtagaaa gtgaatgggc tagagaaata ttagaagaaa tctataagcc ttttaatcaa
    17041 cctataatag ttacaaatag aaatagtgct gagatgataa aatacgcatc aaataacttc
    17101 ttagctctta aaatatcatt tatgaatgat attgcaaatc tttgtgaaat agcaggagct
    17161 aacatagaag atgtagcttt aggaatgagt tatgatgata gaataggaag taagttttta
    17221 aaggcgggaa tagggtatgg cggttcttgc tttccaaaag acacaaaagc tcttcactgg
    17281 ttatctgaac ataatggtta tgaattaaaa acagtaaaag ctgcaataga agttaatgaa
    17341 agtcaaaaac ttaaattagt tagaaaggca tctaagttag tagagagctt tcaagatatg
    17401 aaagttgctg ttttaggttt gacttttaaa ccagacactg atgatataag agaagcacca
    17461 tctataccaa atataaaata tttgcttgaa agaggggcaa gagttactgt atacgaccca
    17521 attggtgtaa ataatactaa gaaaatattt ggtgactcta tactttatgc tgattctgta
    17581 gatgatgcga tagaagatgc agagatatgt tttataatga cagaatggaa agatgtatta
    17641 aattatgata tacataagta taaggaaaag atgaaaaatc ctttagtcct tgatggaaga
    17701 aattgttata aaattaaaga tatggaagag ttaggaattg agtattattc gataggaagg
    17761 taaatattat gaagactgtt tgttatttag ctgatgggtc aaatcctcat actttaaaat
    17821 ggtgtagttt ttttaaaaat aaaggatatg acattcatgt aatctcttta aatggaggtc
    17881 acatggaagg aataaaaatt tataattttg gttctaatgt agaagaatta aaaaatgaaa
    17941 atatttctaa aaaaacggga tacttaggtt caataaagca gataaagaag ctagtaaatg
    18001 aaataaagcc agatatattg catgcacagt atgcaagtag ttatggattt ataggaagtc
    18061 tacttgggta tcatccatat attatttctg tttggggtac tgatatttat gatttcccta
    18121 ataatggttt tatacaaaaa caaattataa aacataactt tagaaaagca gactatatct
    18181 tttcaaatag tagagatatg gctaaagagg ctaataaata tacagctaaa catgtagatg
    18241 taaccttttt tggagtagat atggataggt ttaagcctat ggaagtagaa aaagaagacg
    18301 cttttgtaat aggaattata aagagtttag aaaaaaaata tggaatagaa tatttaatac
    18361 aagcatttaa gatgcttaaa gatgaatata aagataagaa aataattctt aaaataggtg
    18421 gctctggaag tcagatggac aacctaataa atttagctaa agaattgggt atagaaaatg
    18481 atgttcagtt tttaggaaga atatctccag aaaatgtaag caagactttt aattcttttg
    18541 atgtaactgt atttccttct ttaagagagg gatttggagt ggcagctata gaatcagaag
    18601 catgtgaagt acctgttata gttacaaatg taggaggaca tccagaatct gtatgggaaa
    18661 atgaaactgg cttaattgta gaaccaaaac aaccagaaga aataaaaaat gctataataa
    18721 aactaatgga aaatgatgaa cttagactta atatgggtaa gaaagggcgt caatttgtta
    18781 gagaaaatta tgaggttaat ttaaacttta atgatataga aaaaatttat gattctatat
    18841 ttgataaata taaaaaataa aatgaaaaaa tacagcaaaa gatagatgtt gttggctatg
    18901 cttaggtgcg tagcttttgc tgtttaaagg agggcttcta gttatgcgag aaagtataga
    18961 tttagagaaa gaaagctttt ttaataggct ttttaaaaaa tataaattta gacatttaac
    19021 actattaatt ctagatattt gtagcgtact agtagcgttt cgaatgtctt taagcttgac
    19081 aggtaattta aatgcattta ccactaatga tataataatt tcagtgtgta tatatatagt
    19141 acttcatata gtatcattta gattgtttaa gtgctacaac acattatgga gatatgcagg
    19201 agaagaagaa attatatcta tttttgtggc aactttagct tatttaattc cgatttatat
    19261 aataaataaa ttattagggt ttgattatcc tataatgttt tatgtattaa atacaatatt
    19321 tattataatg tttacaagtg gcgcaagaat agcttataga gctataagaa tagtcatgaa
    19381 caagacatat tctagaggaa aggtttcaaa tatccttata attggtgcag gagatgcagg
    19441 agaaatggtt atacaggaac taaaaagaaa ttcagaactt aaaaaagtag cagtggctat
    19501 aattgatgat gataaagata aaattggaag aataatccat aatgtgaaaa ttgtaggaac
    19561 aacttcggat ataaaagctg tggtagaaaa gtacaatgta gatgagataa tcttttcaat
    19621 agcaaacata gagaaacgta gaaaaaaaga gattattgat atatgtaaaa acactaactg
    19681 taaaataaag acaataccag gaatttacga aattatagat ggaaaagttg atataaaaca
    19741 gattagagaa gtagagatag aagacttgtt gggtagagag cctataaaaa caaatttaag
    19801 agagataagt aattacattg agggaaaagt aatcctaata actggaggag gaggctcaat
    19861 tggttcagag ctttgcagac agattgcagg atttaatcct aaagagctta ttatagttga
    19921 taattatgaa aataatgctt actcaataca acaagaatta cttagaaaat ataaaaataa
    19981 attagattta aagacagtaa tagcatcaat aagagaagaa aaaagaatgg atgaaatatt
    20041 taataaatat aaaccagaag tggtatttca tgcagcagca cataaacatg ttcctcttat
    20101 ggaatcgagc cctggcgaag ctataaagaa taatatattt ggaactttaa acatagctgg
    20161 cttgtcttca aaatatagag ctaagaaatt tgtccttata tctacagata aggctgtaaa
    20221 ccctactaac ataatgggag ctactaaaag agctgctgaa atgattatac agactatgaa
    20281 tgctgaaagc cagactgaat ttgttgctgt tagatttgga aatgtgcttg gtagtaatgg
    20341 tagtgtaatt ccacttttta agaagcaaat tgaagatggc ggaccagtga cagttactca
    20401 tccagatatc attagatatt ttatgacaat accagaagca gtaggcttag ttatacaagc
    20461 tggggctatg gctaaaggtg gagaaatatt tgtgcttgat atgggagaac ctgtaagaat
    20521 attggattta gcaaaaaact taattaaatt cagtggattt gagccagatg ttgatataaa
    20581 aattgaattt tctggtctta gaccaggaga aaagttgtat gaagaactac ttatgtcaga
    20641 agaaggtctg ttggatacag agcataaaaa gatttttatt ggtagaccaa tagatgttga
    20701 tagagaaaaa ataacaaaat acttaaaact tttaagagaa attactaaca atgaagaggt
    20761 tgaaaaaata gatggtatta tgagggaatt agtgccaact tatattaaac cagaagatgc
    20821 taatatcaaa gaaattgcaa caactagaga aaagtagaat atatttctaa tttgtaacca
    20881 gggggagcaa aaagaaacaa atatatacta aaaagaaaca aaagattaca aaaagattac
    20941 aaaaagatta caaaaaaggt tgcaatttat tttgatgtaa gatagaatgt taattaggaa
    21001 ttggaaacga aggaacaaaa tgttaaattt tcgaaaggat taggtatttg agattatgat
    21061 gaatattaaa aataaaaaac atatattgaa aaaatttata gctatggtgt taatagcagg
    21121 agttgtcaca gtagaagcag gagcaataac ggctagtgca gcagagccaa ctaattcacc
    21181 tatgagtgca acagtagacc agtgtgattt tttaaatgta agaagtggag ctagtgcaaa
    21241 tgatgcagta gtaggaaaga taaacacagg agacaaagtt gaagttttag aattacattc
    21301 aaatggatgg attaaaataa aaagtgttga caatgttaca ggatgggtaa atggagatta
    21361 tttaactatt caaggtggaa atgtagatgc aaaggtacaa aatgttttaa acttagcatt
    21421 taagcaacaa ggtaagccat atagatgggg agcaactgga ccaaatgctt ttgattgttc
    21481 tgggttcact tcttatgtct ataaaaatgg agcaggggtt aatttaccaa gagtatcaag
    21541 atcacaagca acagtaggaa agaaagtatc aagagcagaa ttaaaaccag gagatttagt
    21601 tttctttggt tcaggtggtt caataaatca tgttggattg tatgtaggag attctaaatt
    21661 catacattca ccacaaacag gagatgtagt aaaagttact agcatggctc caggaactaa
    21721 ttatgcaaga agacttataa ctgctacacg tgttttacaa taataataga tattgattta
    21781 cataggattg aatattaaca ttaaatatat aattacattt aatgttttac atagagattt
    21841 aagagtttag taatattaaa atagtgatga acgttataaa aacagcaaag aagataaatt
    21901 tctttgctgt tttttttata ataaattatt tcatattata atcaaatatc aaatataaat
    21961 tttaactata ttattcaata tttgacagat aatatgaatt ttagatatac ttaataagaa
    22021 gtatattgta gaaaaaataa atttaaaaaa ttaaggggga tgaaatgaaa aaaataagta
    22081 tattagtatt atcattgata atgacactta ctatgtgtag tgtaagttca tttgccgatt
    22141 catcaaatga taaggaaatg agagctgctt ggatatctac agtttataat ttagactggc
    22201 ctaagactaa gaataatgaa gccaagcaaa aaaaggaata cacagattta ttggacaaac
    22261 taaaaagtgt aggaataaat acagcagtgg ttcaagtaag accaaaatca gatgctttat
    22321 ataaatcaaa tataaaccca tggtctgaat atcttaccgg tacacaaggt aaagaccctg
    22381 gatatgaccc attaccattt ttaatagaag aagcacataa aagaggaatg gaatttcatg
    22441 catggtttaa tccatataga ataactatgg cagatgagag catagataaa ttgccagcaa
    22501 accatccagc aaagaaaaat ccaagctggg tagttaaaca tggaaataag tattactacg
    22561 acccaggttt accagaagtt agaaagtaca tagtggatag tatagcagaa gtagttcaaa
    22621 attacgatat agatggagtt cattttgatg attacttcta tccaggagta agtttcaatg
    22681 atactgcaac ataccaaaaa tatggaaaag gacaaaataa ggataattgg agaagagaaa
    22741 atgtaaatac tctacttaga gatgtaaaag cgtctataaa gagtataaag cctaatgttg
    22801 tatttggtgt aagcccagca ggtatatgga gaaataagag tagtgaccca acaggttcag
    22861 atacatcagg taatgaaagt tatgtaggta catatgcaga tacaagagca tggataaaac
    22921 aaggattgat tgactatgta gttccacaac tttactggcc aattggtctt aaagcagcag
    22981 attattctaa attagtggca tggtgggcta atgaagtaaa aggaacaaat gtagacttat
    23041 acataggaca aggtatatat aagcaaggtc aatctagtta tggtggacaa aatatagcaa
    23101 aagaaatagt acaacaagta acattaaata gaaagtatag tgaaataaaa ggtagtatgt
    23161 atttctcagc taaagatata gcaaatagta cttctataca aaaggattta aagagtttat
    23221 attctagtag tgaagaacca gttactccac catctaatgt taaagtggag aaattaagag
    23281 gcgacgaaag atatgacaca gcagtagcaa taagtaaaaa aggttgggct acaaattcag
    23341 atacagtagt attagtaaat gggtactcta tagttgatgg cataacatca acaccacttg
    23401 ctacatcaaa tgatgctcct atactattgg taaataagga taatatacct acatccacta
    23461 agaatgagtt aaaaagactt aatccaagta aagttatttt aattggagga aataactcta
    23521 ttggagataa agttgagagt gagataaaag atacattgtc aaatgtatca ataaatagag
    23581 ttggtggttc agatagatat agtacatctt taatgatagc aaaagaatta gtaaaaacta
    23641 atccagtaga aaaactatat ataacaagtg gtacaggtga agcagattct ctttctatag
    23701 cttcaaaagc aggagaagaa aagcaaccaa tagtactagt atcaaaagat aatgtaagtg
    23761 atgaagttta caattggata tcagatttaa aagtaaaaga tgcttatttt ataggtggta
    23821 atctaagtat atctgattca gtaataaata agctggataa ggtcattaca aatgatgtat
    23881 ctaaaaatag aatagctgga gaaaatagag aagaaacaaa tggaaaagta atacaaaaat
    23941 tctatccaaa tgctgaatat tcatcaatgt ttgtaagtaa aagtaatcaa ttggtagatg
    24001 cattaacatc aggtccttta gcagctaaat taaaatcacc agtagttatg cttggaaata
    24061 gtgtaacatc tgctcagaaa actgctttag agcctaagaa aactgctcta gtatacgaag
    24121 ctggagatgg tataaatcaa aacacattaa acactttctt aaacttagtt aagtaataaa
    24181 aaagttaatt taaaatacag agtattttat aaatgtagat taaaatgaaa attatcatca
    24241 taaattagaa aataaaaaag aactatatgc attttgtaat caagtgtaca cagcaacttg
    24301 aaaataatta tgcgatatag ttctttttta gtaattaatc ttatactttt taataaactt
    24361 tatgttgaat tttttattgt ttaaactcta tgtcattaaa tataaaatcg tgtaatatgt
    24421 ctaaagtatc tgggtcaaat ctaagaaccc atccagcttt tccatatata ccacctgtac
    24481 tatgaatttc atcatcaata ggaaattcaa attgcttaat atttaagtca cccattttta
    24541 aaacttgagc tcctaaagaa agtattgcat taggcttcat gttagtttta acatatggaa
    24601 gaactgcatc taataaacct ggatatttag taacaggtaa agtttccgtt ttcttgatta
    24661 tagcttgtat aatctgtctc tgtctgccat ctctagcaaa ggctgtatcg ttgtgtctga
    24721 tacgtgcaaa agatagtgcc tgatatccat ttagagtctg ctttccagag tttctaatgt
    24781 attgtatgct acctttatca tcacttttat tccatttata agtttcagga ataaatttat
    24841 ttagttctcg tatttctcct ttttcaacag taacctcaac gccaccaata gcatcaatta
    24901 tatacataaa tgattcgaaa ttaacacaag cataattttg aatatcaata ttaaaattct
    24961 cttcaattgt ttgtattaat aaatcagctt gtccataagc ataagcatga gtaagttttt
    25021 gtttaccatg accagggatg tctacatagc tatcacgagc taatgatgta agtttgatat
    25081 cattgtgttt gttatcaata gtaagtatca tcatagcatc tgaccttgaa gaatcttcat
    25141 ttggtctagc atctgttccc ataagtagta tgttaattat cccatcttca tttttatggt
    25201 cattgttatc caataaatca ctacttatgc tcgagtcatg aatagcagat aatttataat
    25261 aaaaatatcc atagacactt atagggaata ttactaccaa gaaagccagt agtataacaa
    25321 atttctttaa ttttgacaaa agtacccctt ctttcttctt tttcctgtat attttttaat
    25381 agcatacact taattatatt aaagtattga gatatattca atacttgttt cagttacaaa
    25441 aaaaagacaa tgatataatt tataagaaaa tttatcgacc tatatgtata gttaaaagta
    25501 tatataatat attttttaaa agtaatttat attttaaaat aaaaacacac cccataaata
    25561 caaagcaatt tgcatttaag aggtatgtat ttatcatcaa attattggtt tgttttggaa
    25621 tttctaatct tcaaccataa tatctttaaa tatgaaatca tgcattgcat ctagttcata
    25681 ttcctcaaaa ggtattacat agcctgcatt acctattttt ctaccatttt cagtagaaag
    25741 aggaaactcc atagatttca aatttaaatt tcctatactt aaaagttctt ttcctaaaga
    25801 tattatttca ttaggattca tattagtttt tacatacgga agtattgtgt taactagatt
    25861 aggatattta gtgactggta aatccttaac accatttatt aaactctgta taacacttct
    25921 ttgtcttcta tctctttcat ctgtactatc attttttctt atacgagaaa atgcaagtgt
    25981 ctgatatcca tttaattttt gctcaccaga atttcttata tatttaagag aacctttatt
    26041 gtcattttta ttccatttat aagtttcagg aataaattta tttaattctc ttatttcact
    26101 cttatcaaca gtaacagtaa ctccacctaa agcatcaact atatccataa aagagaaaaa
    26161 gtcaactacg gcgtagtttt gtatatcaag ttggaagttt tcttctatag tttctattaa
    26221 taagttagct tgtccatata cgtaggcatg tgttaatttt tgttctccac gtcctggtat
    26281 atcaacataa gtatctctat ttaatgaagt tagcttaaga cttttatttt tattatcaat
    26341 agtaagtatc atcatggcat cagaacgaga aacttcttct cctggtcttg catctgtacc
    26401 taacaataat atgtttgtga tattttcttc gtttttatag tcattactac taagcgcctc
    26461 tgtatttata ctatcatctt gtatagtttt taatttagta aaaacatatc catatatact
    26521 aacaggaaat gctataacta aaagggctag aaatataacg aactttttga gtcctgacaa
    26581 taatatcccc tactttctaa attttttaat tacacatagt taattatatt aaaaatttgt
    26641 tacataagca ataaaaaaca caataaatta gcataagttt gtcataatat aattattttt
    26701 atagtatttt atagaagaaa gtgtgtagtt attattgatt ttattggaat ataattgtat
    26761 gaagacttaa atttttataa actgatgtgt atattgaaaa aaatattttc ctgaatgtag
    26821 ttatagcatc ttatgaaagg tgtaaattgt aatttaaaat ttttagtgta taataagaat
    26881 tgaaaattca aaccgataaa aattaataaa aaataattat aaattaataa tttgggggag
    26941 gaaatgtatg ataaaattaa tagcaacaga tttagatgga actttgttag atgaaaagag
    27001 cgaaataaat ccagagtttt ataaagtatt taaaaaatta agagagagag ggataatgtt
    27061 ttctgctgct agtggcaggc agtatcaaaa tcttataaaa aaatttgaag atataaaaga
    27121 tgatatgatg tttatatcag aaaatggaac actagtagta tataaaggaa aagaaatatt
    27181 atcaaatcct ttaaataaag aattagtaaa tgaaatcata gagacaacaa gaagtataaa
    27241 agggaaaaaa attgtaatga gtgggaaaaa atacgcttat atagaaagca aagatgaagc
    27301 gtttatacaa gaggttagta cttattatgc aaagtttaaa attgtagacg atttgacgaa
    27361 agtagaaggc gatatattga agatagctgt atttgacttc aaaggtgcag aacataataa
    27421 taatatctac tttgaaaagt tttctgatag agctcaagta tgtatttctg gagtggagtg
    27481 gcttgattta actgcaaaag gtgcaaataa agggagtgcc ataaaaaaag tacaaaaaat
    27541 gttagatata aaatatgaag aaactatggt atttggtgac cagttaaatg atgtggaaat
    27601 gatgaaaagt gcctatcata gttatgccat ggaaaatgca aatgaacatt tgaaacaaat
    27661 tgctagattt agagcaaaaa gaaatactga aaatggcgtt gtagataaga taaaagaggt
    27721 cataaaaatt ggatagtcat atgtatagat gaaaaagatt attttgaaga aatcaaaaaa
    27781 atatcaatgt aattttgata aataagttta tttatgcgaa aattacattg atatttaata
    27841 aacatcaata atagatattt atttttttat gatattcagt attttgtttt ctatttgagt
    27901 tgcattttca gcagaaatta aataaacata tctacctgct ttctcaatta tagtattatt
    27961 tgctatttca gaattgattt tttctccatg tccttctcca aaaggtgctt gtatcatatc
    28021 ttttttataa ttttctagag ttttatatat tttatctacc tcgtcagttt ttataacgat
    28081 aatatcttct agagataatt tttcagaaga attaattaca aaaccttgaa ttatgtcttc
    28141 ctctacatca ttaaaatagt taaagtcctt tgcatcaaag atttttgatt cttttataag
    28201 taaatcagaa gattttaatg ctttttttat ttttgatgta gaaacgtttt tgtaatcacc
    28261 acttgaagaa caactaacca ttcctaatga taagattatc gaaaaaaaca atattaaaat
    28321 ttttttcatg ataaacctcc aagtttttta aactctgtct taaattagtt tactacataa
    28381 ttatataaaa ataaatgtat ttaatgttac agttttgatt aataatgtaa tattttgata
    28441 ataataccta ttgagaggtg attagaaatg agaataccaa aacatgtagg aattataccg
    28501 gatggaaata gaaggtgggc aagtcaaaat ggtatgacaa aagataaagg atatgaaagt
    28561 ggaatagacc ctggattaga gtcttataga ttatgtagag atttaggctg tgaagaggtg
    28621 actttttatg gatttacaac tgataataca agacgtccat cagtgcagac aaaagctttt
    28681 acaaaagcat gtattgatgc tgcaaatata ttaatagatg aaggtgctgc tttattagtt
    28741 gtaggaaata caaaatctcc aatgtttcca gaagaattat taccatatac taatagaatt
    28801 aaagccaaag aaggtcaaat aaaagcaaat cttctggtaa attatgggtg gtattgggac
    28861 ttaaataata tatcaggagt agaaaatgta aataaaaata atatacagga ttacatacag
    28921 tcatttgatg tatccagact agatttaata atcagatggg gtggaagaag gagattaagt
    28981 ggtttccttc ctgtacaatc aatttatgca gatttttatg ttatagataa ttattggcca
    29041 gattttaaga gtgacgatat aaaggacgca tttagttggt atgatacaca agatgtcact
    29101 ttaggtggat aattaaaaat attctgattt aaggttgtag agtgttttaa attattgaaa
    29161 aaaaaacttc ttgatggtaa aataacacta ataatatgta aaagtgtaaa aatttatacc
    29221 aagaagggtg aagaaatgtc aaaaggcaat aataacaata attcaagaaa caaatctaaa
    29281 aagaccagtc atctgaatag aaaaaagcga aaattaaata agaaaaagtt agcagtgttg
    29341 atttgcttta cagttttatt tttatttatt gcatttaagg ctactcaagg tgtagtagct
    29401 ttggtaaaaa gtatggataa aagtaataaa actagtcaac aacaaaaagt taactcagaa
    29461 caatttgacc ttggtaatga agaggaaaat aaaaagaaaa aatatactgt atttatagac
    29521 ccaggtcatg gaggcaatga taaaggaact gaaagcaaga ccagtaatag atatgaaaaa
    29581 gacctaaatc tacaaattgc aaaaaaatta gcaaataaat tatctaaaca aaaggatatt
    29641 caagtagtag tatccagaac tgatgataca tatatatcat taaaggatag agctatctta
    29701 gcaaataata gttctgctga tgtgcttgtt tctattcatt taaatgcaga gaaaaatggt
    29761 aatacagcaa caggtataga aacttggtat aggaataaag ctacagatgg ttcaaaagaa
    29821 cttgcacaga cagtacaaag tactatagta tcctatgtta aagttagaga taggggaata
    29881 gttgaaaata actttgaagt acttagagaa tcaaatatgc ctgctatatt aatagagtgt
    29941 ggttttttaa caacaccttc agaggaacaa aaaataataa atgaaaaata tcaagaccaa
    30001 cttgcggaag gaattgttca aggtgtactt tcatacctag attctaaagg gaataagtaa
    30061 gttaacttta ctttaatgtg aggataaatt taaaagttta actgtgtaaa ttgtattaac
    30121 tttatttcgt aatgttggaa acattgtttg aatttagaaa ctatgaaatt agaaattatt
    30181 ttatctatat ctaatttcat agtttttttt gcaaaaaatt tatagttata aaatttaaaa
    30241 aaat
//
LOCUS       DAMJWP010000021        29594 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.022, whole genome shotgun sequence.
ACCESSION   DAMJWP010000021 DAMJWP010000000
VERSION     DAMJWP010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29594
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.022"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(91..2061)
                     /gene="ftsH"
                     /locus_tag="PC700_000875"
     CDS             complement(91..2061)
                     /gene="ftsH"
                     /locus_tag="PC700_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDF2580198.1"
                     /translation="MNKLLKGAGFYLLVFIIIVGIVQFSGKPTEKIKDLKFSEVYREL
                     TDENISRLYFVNQTSVEGTIKDTNTKFKSYVPTEVMGNKLADEVLDQAKAGKLTFGGE
                     AKPSTPWFVEMLPTLLLIFFMVIIWFVFMNQSQGGGGKVMSFGKSKAKVHKDDEKTRV
                     TFKDVAGLDEEKEDLQEVVDFLKNPKKYIELGARIPKGMLMVGPPGTGKTYLSRAVAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKSAPAIIFIDEIDAVGRKRGAGLG
                     GGHDEREQTLNQLLVEMDGFGVNQGIIIMAATNRPDILDPALLRPGRFDRQVVVGTPD
                     VKGREAIFKVHSRNKPLSDDVKMDVLARRTPGFTPADIENLMNEAAILTARKREKKIK
                     METIEEAITKVIAGVAKKSKVISEKERRLTAYHEGGHAVCAHVLEEVSPVHQVTIVPR
                     GRAGGFTMQLPVEDKFYATKNEMKENIVVLLGGRVAEELVLKDVSTGASNDLERVTAT
                     ARSMVTKYGMSSKLGPMSFDSDDEVFLGNSFSSKRNYSEEVAFEIDQETKRIVDGAYD
                     KTRSILQENMDRLEYVAQALLIYETLDAEQFVKAFNKELPLNEIENAVTEENSSKEVE
                     EQLTIKLEKDEEERNNVIDINKNLEDKSDKDK"
     gene            complement(2095..3483)
                     /gene="tilS"
                     /locus_tag="PC700_000876"
     CDS             complement(2095..3483)
                     /gene="tilS"
                     /locus_tag="PC700_000876"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDF2580199.1"
                     /translation="MIFDKVLSTINKHNLIQKGDKIVLGLSGGPDSVCLLHVLNRLKK
                     DFNIEIYAAHLNHQIRGIEAQKDALYVSKLCEDMGIIFFVKSINVPKYCENEGLSLEE
                     GARKLRYEMFYEIKDKIKANKIAIGHNLNDQAETVMMRIMRGTGLKGLKGIDYIRDNC
                     IIRPILDVERNEIEEYCEAYNLNPRIDKTNLENIYTRNKIRLDLLPYMKDNFNSNVIE
                     SIVRMSNSLKSDNDYIEKEAEAKFREVSNIKEKGFVEINLDDFVCLHDAIKVRVLRNS
                     IKHILGDTNFVDQRHIEDIMSLEDNSKVNKMLTLPRNIFVYRKKDSIILTNEEIVNEE
                     IEFYYNVPSNGFIKIKELKQIIETQVMSIDRYKSMKLDNSSKGFDFNKVKGGIVIRSR
                     RQGDKIKLAMGSKKVKDLFIDLKIPREERCKIPIITDSEGIICVGDYKISENYKIDEN
                     TKEVLKINFNKL"
     gene            complement(3666..4094)
                     /locus_tag="PC700_000877"
     CDS             complement(3666..4094)
                     /locus_tag="PC700_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HDF2580200.1"
                     /translation="MEEKSLSVKISINTRQYDEKGNMDTIEMTAFGKIFYKNDGIYVI
                     YKEKEENIEITNTIKILKNQVSIKKFGAINSTMLFKCGESSTTKYVTPQGTLLIDIDT
                     RELDINIQEGEHIKLKIDYNIKVQDLFLGRNKIDIYIGTK"
     gene            complement(4111..4917)
                     /locus_tag="PC700_000878"
     CDS             complement(4111..4917)
                     /locus_tag="PC700_000878"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDF2580201.1"
                     /translation="MNNKPIGVFDSGLGGLTVLKEIMKILPNEDIIYFGDTARIPYGS
                     RSKETIIKYTFQAINFLKTKGVKAIVIACNTATARSLKEAQEKYDIPIIGVIEAGART
                     AVSSTKNKIVGIIGTEGTISSKAYNLEISKIDESIEIVNKACPLFVPIVEEGWANTEV
                     AKLTAKIYLQELKEKNIDSLVLGCTHYPILKRTIGEEVGEHIKLVNPAKETAKDLKKI
                     LEVQNIINNTEIHGTYQYYVSDIPEKFSDIAREFLKKKIDKIQKVEIQKY"
     gene            complement(5119..5604)
                     /locus_tag="PC700_000879"
     CDS             complement(5119..5604)
                     /locus_tag="PC700_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HDF2580202.1"
                     /translation="MATLLALTFIFMPIKEVFAYEPTENIKKVNKQEKNIAKKEVINL
                     SDNDLMLLSKLVAGEARGESYEGQVAVAAVVINRVLDPRFPDTIEEVIYQKNAFSVVL
                     DGSIKMAPTDSARKAAQEALYGTDPTNKAVYFWNPEIATCKWINRLNPYMRIGNHVFA
                     K"
     gene            complement(5655..6314)
                     /gene="spoIIR"
                     /locus_tag="PC700_000880"
     CDS             complement(5655..6314)
                     /gene="spoIIR"
                     /locus_tag="PC700_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HDF2580203.1"
                     /translation="MRKIKIRLGLLILSLISVISIMTIVINGEVKKVDNISKDYKDKL
                     IRFHVIANSNTDEDQELKLKVRDEVIKYLQPKLQNSKSIKESEAIIKKEYSSLEEISK
                     NIILKNGYNYSVKVGIQYSNFPTKQYSNIVLPAGEYKALKIIIGKGEGKNWWCVMFPP
                     LCFVDESNGVIDKSTDDKLKEVLTDKEYKLIKQDTPKKTSRVKIKFKVLEVVKDLEKK
                     F"
     gene            complement(6459..7139)
                     /locus_tag="PC700_000881"
     CDS             complement(6459..7139)
                     /locus_tag="PC700_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2580204.1"
                     /translation="MENLTKLNLDNYKPLRDVVFENLREAILEGKLKPGQRLMEVQLA
                     EQLGVSRTPVREAIRKLELEGLVVMLPRKGAYVANMSLKDIMDVLEVRASLEGLAAYL
                     AAERISDEDIKKLKDISEEFKKSTLESDVDALLKLDVEFHECIFKATNNKKLIQLINS
                     LWEQVYRFRVTYMSAYDTSHNIVEEHKYLLDAIIKGDSELSKKYATEHIEKAEQFMID
                     KAMISKDL"
     gene            complement(7123..8013)
                     /locus_tag="PC700_000882"
     CDS             complement(7123..8013)
                     /locus_tag="PC700_000882"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDF2580205.1"
                     /translation="MDFIRLKSRAKINLSIDVLGKRQDGYHFVEMIMQTIDLYDIVKI
                     KELDEDEIKVKSTSLDIPLDEDNIVYKAAKILKNKFYIKKGVEIFIEKNIPVAAGMAG
                     GSSNAAAVLVGLNHLWELRLSEDELKEIGLNLGADVPFCISGRPALAQGIGEKLTNIK
                     GLPCDTNILICKPDLFVSTKEVYQGLDLNNIKKRPNNKYLIECLKSEDIKAVSESMVN
                     ILENVTIGKHKEISDIKQVMMKNNALGSMMSGSGPTVFGLFKNKEDALIGKKELLKKY
                     KQVYVVNSSQKGVEICGEFN"
     gene            complement(8131..9681)
                     /locus_tag="PC700_000883"
     CDS             complement(8131..9681)
                     /locus_tag="PC700_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HDF2580206.1"
                     /translation="MELIKDVIKVDNRIDFGKFQTFIEAEAVVPDKKLDVYEIVKTEG
                     YISLKKIEMADGKILCRGSFNYNVIYIADDKNTISNVDGKVDINEVIEKDNVVQDMEY
                     MLFSEVEHMDCTIMNERKIKVGALMNIRGSLFEKQRLDIVKDVAQVEGIQKHRKEICF
                     QDIVGIEKAESSIRDTITINTEEIQSIISLNPCVKVKESRVTDNKVIIGGVLEINPLA
                     CTYEGELVELDRVGIEFTQFVEVPGVSDGMTEEVLLSMSDFNHIFKQNSESNTGLLEI
                     DCMACCKVKVTDEVTREVLQDAYSPQKIIKFDHKPIQLNKTLRRSEETFMVREGIRND
                     NDDIQIKDIVSVCPTMSIENSYIEGNKSIIQGIIKVEILFVPVEGLKLVYKISEEIPF
                     EHDIEMDNLTDTASVFNTACIDKVEVDLNRDQIDLIIKIKRFTEALDKKAENFIIKGE
                     DQGVYDLSKAPSIIVYICKEGDTFWNIAKKYNTTENEIAELNDIKLDEPIKPGKCLIL
                     EKKVVLVD"
     gene            complement(9775..10041)
                     /locus_tag="PC700_000884"
     CDS             complement(9775..10041)
                     /locus_tag="PC700_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HDF2580207.1"
                     /translation="MATVQTLDKIRVSLERHIGKKILLKANKGRKQIITKEGILEKVY
                     PSVFVIKLDDESNGYPRVSYSYSDLLTSNVKLQVFRDQDKLQIS"
     gene            complement(10189..11049)
                     /gene="yabG"
                     /locus_tag="PC700_000885"
     CDS             complement(10189..11049)
                     /gene="yabG"
                     /locus_tag="PC700_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HDF2580208.1"
                     /translation="MKVGDIVARKSHNKDIVFKIVSFGVDENNEKIAILKGIAFRVIA
                     DAYIDDLELVKAPDIKDILIDKNVENLLYKSVRKAKERQKRMTRAVPKLQLNPNTYGM
                     PGKVLQIDGDKEYLKICLDVYAQLGIPAVGVAVSEQNQYKEVRALLEKYNPDILVITG
                     HDAMTIKRGDIEDMSNYRNSSNFVKTVKEARKWQPNLDNLVIFAGACQSNYEKIISAG
                     ANYASSPGRIMIHALDPVFIVEKIACSRIDVVVPIDEVIEQTITGVKGIGGSETRGKF
                     RWAMPKTILH"
     gene            complement(11133..12071)
                     /locus_tag="PC700_000886"
     CDS             complement(11133..12071)
                     /locus_tag="PC700_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HDF2580209.1"
                     /translation="MKFIDLHCDTIAKLMENVETSELKSNKYSVDIDRLKKGDSLAQT
                     FALFVDTEEVKHPFDYCMSMANKFHEEMKKNSDEIALATNYEEIMKNQSEGKLTALLS
                     IEEGAVLEGKLENLKKFYDLGVRMMTISWNHVNELSFPHNKVEYREKGLTDFGREVVH
                     KMNELGMLVDVSHISDGGFYEIAKISSKPIIATHSNSRAMMNHSRNLTDDMIKVLANK
                     GGVTGINFFHLFLSDKSESKLEDMVRHIKHIVNVGGIDVVSLGSDFDGIDSKVEIEDI
                     SQMGKLYEPLKKEGFSEDDIEKIYYKNALRVIKEVL"
     gene            complement(12181..12342)
                     /locus_tag="PC700_000887"
     CDS             complement(12181..12342)
                     /locus_tag="PC700_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580210.1"
                     /translation="MFILRFADDDDNLSVKDFTSLTDLKKYISENNIDKTWHQIEEVK
                     KVIPNLKEE"
     gene            complement(12352..12849)
                     /locus_tag="PC700_000888"
     CDS             complement(12352..12849)
                     /locus_tag="PC700_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HDF2580211.1"
                     /translation="MSLYTEWRNLSDNHESQEAEVKFWEEYLKVEAGIYNEILNNKMD
                     VVEGKVSELAEKFGTTNEFFMGFIDGISESLKEDIVLEEVEADKEVSIKIDWEKLYNN
                     MVTVEAHWLYNLQGWEDILPADQRKEIIKAYKKSKTIVKEAKIGRNDSCPCGSGKKYK
                     KCCGK"
     gene            complement(12964..13674)
                     /locus_tag="PC700_000889"
     CDS             complement(12964..13674)
                     /locus_tag="PC700_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0489 family protein"
                     /protein_id="HDF2580212.1"
                     /translation="MENYEYSGFYIEKPVGNNVFSYDKRENKSIYVPKLINGTLNDVR
                     LGNEVVFNEVDENKEIKAKGLENMVEYVLGNKKIYVFDNHNHAFYFWAKSLLKGEFTK
                     GCKLVHVDQHKDTREPENYDVDVNNLKDVFRYTNEVLNVGSFIKPALKYNIFSELIII
                     DSLYGFDLEVESEFVLDIDLDIFSSDMDYIPFELKFYKIKNLIKKAKVITIATSPYFI
                     NQEYAIKVLKELFNYDII"
     gene            complement(13850..14380)
                     /locus_tag="PC700_000890"
     CDS             complement(13850..14380)
                     /locus_tag="PC700_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580213.1"
                     /translation="MDIRALPIRITKEEAIEIAKSGKNSLFSKLILKNKYAHEVRLHY
                     IEFKIITLKIHKTKSIFKNRRYSNDINELKILVNGSTGSGSIVQDFPTYEIVKNVDCN
                     IVQYSNKDERRVKAKANKMAMRLIHRFMGGIPEIEIVKIESIFRPYWVVFFGKVELNR
                     RVSYLPIEADGFVIGM"
     gene            complement(14397..14627)
                     /locus_tag="PC700_000891"
     CDS             complement(14397..14627)
                     /locus_tag="PC700_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580214.1"
                     /translation="MSKLVSKVKKDLEIKWLGVDGFLVLLYFLITGFIFYGILTRNDI
                     IVGKIIPYGVMAVVIVIFFDYIYRVLKMTRKK"
     gene            complement(14642..16036)
                     /locus_tag="PC700_000892"
     CDS             complement(14642..16036)
                     /locus_tag="PC700_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HDF2580215.1"
                     /translation="MLTFWENGIIVIFAFILLGIGYYFSRNVKDMESYYLGNRSLPWS
                     LIVGTLVASWYGGVGTVGSVEYAAIYGISAWAVWSIAAHIGRMPLALWVGPRIHIRTN
                     ITVPDLLESFYGKHVAIIGAILMFIYCAQLGEITAMGTIGNVAWKIDKELIGLIMVGV
                     VVVLTVLGGLMGVAITDMVLFFCMVFGLTMVMPGQFEQIGGFQGLTEALKDAPQLMHP
                     TKGMSAMKALMLIVYSFGAYSDPTFYQRFSAADSPKVGRRALLTCFSLWICFDMVLTI
                     TGLIVRATYPEMAPAAGYITLVLDTLPQGIRVIFIIGILGSIISTLDSYYLAAGATLA
                     NDIYGRITKKELSQKQLVKFTRIGVCLAAVMGLSVAFRFENVYDACIFVGSIWMGSAF
                     VPIVGGLLGNGRRTSMGGILGMIVGGVTFGYFKIFPVQSFELEPLVIAIPFSFIAWMI
                     GNRIGENLNTQNNL"
     gene            complement(16162..17415)
                     /locus_tag="PC700_000893"
     CDS             complement(16162..17415)
                     /locus_tag="PC700_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2580216.1"
                     /translation="MENKSNFKRFLIIFILAFGTTAMYSLPYMKSSFYDPMQQALSLS
                     HTQIGNLLSLYGLVGMVSYFIGGWFADRFSVRKLITFSLIASGVLGFYFSTFPSYSMI
                     LLIFVLWGFTTILTFFSASVKVVRMQGSESEQGRIFGFYEGLSGVSGTLISFIGLYFF
                     GKFAEITVGFKYVVWLYSAASIICGILLFFLVEEKKDSGASDEGLSIKSLLKVVTMPK
                     AWLIGLIIFSTYLVFSSLTYLSPYLSEVYVMPMTLVSALSIIRTYVIKMGASPVAGVI
                     TDKVGSSIRVMFVGFILMTVSTAAYLVIPKSTGFIWIAVINMIILSVILFGFRGIYFA
                     SVSESNISLETTGAVVGFASFIGFSPDAFYYTIAGNWLDKYGQTGYTYIFILSVVCAV
                     IGIFATYALNKINKRENKGLNNKIA"
     gene            complement(17466..18620)
                     /locus_tag="PC700_000894"
     CDS             complement(17466..18620)
                     /locus_tag="PC700_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2580217.1"
                     /translation="MSNNINQDLETKIIHWGHSADPTTGALATPICQTATFAAKTVEH
                     FEELCMTWGYVYTRECNPTLTELEAKLAMLENAESAISSTSGMGAITSTILALVKSGD
                     HIVSSDGIFSHTKLFMSELLSKFGVEVTFVDAVNPQNVKEAMRPNTKIVYIESPLNPS
                     LDLVDIKTIAEIAHENKSLAIVDSTFGTPIVQRPIDLGADLVIHSLTKFINGHGDTLG
                     GAVAGSKELIDLVRWPSLCCFTGASLPPMSAWMILRGMKTLDMRMKKHCENGLAVAEF
                     LEEEENVELVKYPALKSHPQYELCKTQMNGLGGGVVSFKLKDGINGLTRDQASRKLMN
                     SLELATIATSLGEEHTLVQMNGENLIRIAVGLESSNDIINDFKQAIKKLK"
     gene            complement(19179..19748)
                     /locus_tag="PC700_000895"
     CDS             complement(19179..19748)
                     /locus_tag="PC700_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF2580218.1"
                     /translation="MIIIYGYCRVSTKNQTDNNSLYQQQQEILNKYNDAKIIKETYTG
                     TTTNRPKFEKIISSLKKGDLLVATKLDRLARSTSEGIQLIEGLFAKEVSVHILNIGLL
                     ENTSMGKFFLTTLLAVAELERNMIIERTQLGKEFAKKKKDFKDGRPKKYSNQQIQYAL
                     GLLNSKPYREVVKMTGISKSTLIRAKKSI"
     gene            19951..20178
                     /locus_tag="PC700_000896"
     CDS             19951..20178
                     /locus_tag="PC700_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF2580219.1"
                     /translation="MNSLLKEAIDELKNIHCGEVFLLKELFKGYEWNRLEMNIRLKLG
                     KLFLYEVVTNPNLNVEVINKNSAYQQMYKKK"
     gene            complement(20397..21140)
                     /locus_tag="PC700_000897"
     CDS             complement(20397..21140)
                     /locus_tag="PC700_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDF2580220.1"
                     /translation="MENYQSTNTCTYNILYVQFNEDFERSKYFSYDVKYPVIYNIRNQ
                     STYVNPVILNNINNAVRMTVNNFKNGLQEEEREYNNAASQNSLPKQSYWVSTSYAVTF
                     NKNYVLSMILNLMGFANNYGPKYNTLNNYNIDLTTGKELLLNDIFRDGVDYINIITDY
                     IKSQINENREMYYSNVELVIPEDQAFYITDNGIIIYFGVDEIAPESFGIPKFELKFSD
                     FGNFINLRFYCISPDIYVQSRGRAKHFMG"
     gene            21308..22180
                     /locus_tag="PC700_000898"
     CDS             21308..22180
                     /locus_tag="PC700_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2580221.1"
                     /translation="MLLLADKWKDYELIDMGNGEKLERWGSYVLRRPDPQVVWPMESE
                     WALWKNPHGHYHRSNKGGGQWEHKKRYPEQWTINYKNLKFHIKPTGFKHTGLFPEQAA
                     NWDWMIEKIKKANRPIKVLNLFAYTGGATVACASAGAEVCHVDAAKGMVNWAKENIEL
                     SGLKNQTVRFIVDDVVKFVEREIRRGKKYDAIIMDPPSYGRGPKGEVWQIEEKLYSFV
                     DLCMGVLSDNPLFFLINSYTTGFSPIVLENVLSTTIGKKTNTGDIYGGELGIPASRDG
                     KILPCGIFGRWEKK"
     gene            22180..22845
                     /locus_tag="PC700_000899"
     CDS             22180..22845
                     /locus_tag="PC700_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDF2580222.1"
                     /translation="MIKVIYEDNHLLVVEKPVNILSQGDNTNDKDMVNLLKDYIKKKY
                     NKPGNVYIGLIHRLDRPVGGVMVFAKTSKAASRLSEQVRSKTFKKTYLAVVHGKIKSN
                     SDILKDFLYKNKKTNMVTVVKKSHKDAKEAELNYNVLDFKENLSLVQVNLKTGRSHQI
                     RVQFASRKHPLYGDQRYGKEINKIGQQISLWSNKIEICHPTTKEKMEFTCHPPDKYPW
                     NIF"
     gene            22866..23723
                     /locus_tag="PC700_000900"
     CDS             22866..23723
                     /locus_tag="PC700_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2580223.1"
                     /translation="MSKKFSIGEMSKLHKISIQTLRYYDQIDLFKPVHVDQESNYRYY
                     SIDQFSQLDIIKYLKYLGMPLKEIKSKLDENNEEILKLLTNKINIVDEKIKELELIKK
                     VLSTKKETMEKGMKVDEVGKIVRKHIPRRSILSIEYEEGLSSEETMELSRRQIANILE
                     DNISVFYGGVSGLVSIDKFLKEDKVIYDNSFVTVERDLFDIKSQKHITEISSGDFICM
                     TYKGSYKENYTAFKKLVDYVEYKRIPIENYIYEIPIIDPLSTNNEEELLTEIQIQVKK
                     REIDLRCLV"
     gene            complement(23879..26425)
                     /locus_tag="PC700_000901"
     CDS             complement(23879..26425)
                     /locus_tag="PC700_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2580224.1"
                     /translation="MNIFNKVALQGLKKNRTRTLVTIIGVVLSAAMITAVTTFGVSLM
                     NYMTNGAIIKYGDWHVQFLDVDSSFVQEQRVNDEVANTATFENIGYATLEGNRNPDKP
                     YLFIAGFSKEAFDTLPITLISGRLPENSREILVSSKVATDGGVKFAVGDTISLDVGKR
                     MSENEKLGQSDPYTAGSEVFVPQDKKTYTVVGICRKPVFEESSAPGYTLITKEDKQVK
                     TDSLSLFVTLKNPHQIRSYTSSTKDHASIFNDNVLRFIGVSDNNLFNMFLYSVGGIVL
                     AIIMLGSIFLIYNSFNMSLNERTHQFGILSSVGATAKQLRNSVLFEGICIGAIGIPIG
                     VAIGIVSIGLVISVIAKNFANILYVNVPLTLTLSIPAIIVAAVVSMVTILISAYIPAR
                     KAANTPVMECIRQTNEVKIESKSVKTSKLTQRVYGLEGVLAMKNFKRNKKRYRSIVLS
                     LVLSIVLFISTSALVADLKQMSNQRSEVTDQDIIFSTQGMKDNEMLKLYDKLKTANGV
                     YEGLYQKLMTYSCVAKVSELTDDYLNYEGSHSMSEMLELPVDMQFLDDRTYLNIIKSL
                     GLPAEEYTGQNAKLIAVSMVTKDNNIIDIFNSSSMNVSLTPETNGKLEIPERQNVNIS
                     FVKVVPPDSPPGIRSSDVKKASFRVLAPYSAKDKFEIPGVSTGIESLSFQSKNPTQSV
                     AEMEQIIESEGVTNEYNLYNMYGMMDENRNYIFIANVFAYTFIIMISLISIANVFNTI
                     STNIKLRRRELAMLRSVGMSEHDFQKMMNFECAFYGMRALFFGLPIAVISSWLIHKVM
                     VTDDIDFVIPWFSIGISIFSVLFIVFITMLYTINKIKKENIIDALRDDMA"
     gene            complement(26425..27111)
                     /locus_tag="PC700_000902"
     CDS             complement(26425..27111)
                     /locus_tag="PC700_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2580225.1"
                     /translation="MAFLKIEDLCKVYGKNENKVTALDHVSLTIEKGEFTAIIGSSGS
                     GKSTLLHSIAGVDVPTSGKIYLEGQDVYGQSNEKLAIFRRRQVGLIYQFHNLIPTLNV
                     VENITLPILMDKRKVNQERLNDLLELLGLKERKTHLPNQLSGGQQQRVAIGRALMNAP
                     AVMLADEPTGSLDSKNGQEIIQLLKESHSKYHQTLIIVTHDENIALQADRIICISDGK
                     VVRDERKVNR"
     gene            complement(27194..28183)
                     /locus_tag="PC700_000903"
     CDS             complement(27194..28183)
                     /locus_tag="PC700_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2580226.1"
                     /translation="MFRNREFRKFAILFLLITILTVALGFAISIMTGILSIVSATTFG
                     IAFFVFTKNRYKSIAQISEQINLVLHNANHLYIAESDEGELSILQSEITKMTLRIREQ
                     NYALKKEKEHLADSLADIAHQLRTPLTSVTLILSLLENTSDEDERKELIRETEELLIR
                     MDWLITSLLKLSRLDAGIVVFQKEQIDVNNLISSALHQLLIPMELHNITLHIDIPKGV
                     RILGDLNWLSEAIQNIFKNCMESVGDNGKIDIVCEDNFLFTQLTIHDNGAGFKKEDLP
                     CLFNRFYRGKNSSTAGYGIGLALCKTIIMRQGGTITAQNHPQGGAIFVIRFPK"
     gene            complement(28170..28856)
                     /locus_tag="PC700_000904"
     CDS             complement(28170..28856)
                     /locus_tag="PC700_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2580227.1"
                     /translation="MKRIFLVEDDKAIAKNLILLLNSEGFIVTHVSTRSEALVEIAKN
                     KFDLALIDISLPDGNGFTICTEIKEKQDIPVIFLTASGDESSVVTGLNMGADDYITKP
                     FRPRELIARIRTALRKSGRFGSTFEICGLYVDTATGVVKKNGNEVFLSALEYRLLLVF
                     ISNPKSIITRVRLLDELWDAAGEFVNDNTLTVYIKRLREKIEDDPSSPQIILTVRGTG
                     YRLGGSYVSK"
     gene            complement(29356..>29594)
                     /locus_tag="PC700_000905"
     CDS             complement(29356..>29594)
                     /locus_tag="PC700_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580228.1"
                     /translation="IEEPRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKMRRTAIEF
                     STEIMTSLDTLKALVEVKKKHLNKDKLKVYNIAE"
BASE COUNT         9622 a         5366 c         3408 g        11198 t
ORIGIN      
        1 ataatttgct tattaattta atattatact ttcagtttta gtatttaatc cacatcagat
       61 aataatctta aaatgaccaa ggtcttaaaa ctatttatct ttatcagatt tatcttctaa
      121 atttttattt atatcaataa cattgtttct ttcttcttca tctttttcta attttatagt
      181 taattgttct tcaacttctt tagaagaatt ttcctctgta actgcatttt caatttcatt
      241 cagaggtaac tctttattaa atgcttttac aaattgttct gcatctaaag tttcatatat
      301 aagtaaagct tgtgctacat actctaatct atccatattt tcttgtaata tacttcttgt
      361 cttatcatat gcaccatcaa ctatacgctt tgtttcttgg tctatctcaa aagcaacttc
      421 ttcagagtaa tttctcttac ttgaaaaact attacctagg aatacttcat catcactgtc
      481 aaatgacatt ggaccaagtt tagaactcat tccatacttt gttaccatac ttcttgcagt
      541 agctgtaact ctttctaagt catttgaagc acctgtagat acatccttaa gtactagctc
      601 ttctgctact ctaccaccaa gtaaaactac aatattttct ttcatttcat tttttgtagc
      661 atagaactta tcttcaacag gaagttgcat tgtaaaccct ccagctcttc ctcttggaac
      721 tattgtaact tgatgaacag gacttacttc ttcaagtaca tgggcacaaa ctgcatgccc
      781 tccttcatgg tatgctgtaa gtcttctttc tttctcactt attactttcg attttttagc
      841 cacacctgct ataacctttg ttatagcttc ttctatagtt tccatcttaa ttttcttttc
      901 tctttttcta gctgttaata ttgcagcctc attcattaag ttctctatat cagcaggagt
      961 aaatcctggt gttcttctag ccaagacatc catttttaca tcatcactta atggtttatt
     1021 tcttgagtga actttaaata tagcctctct accttttaca tctggtgtac caacaacaac
     1081 ttgtctgtca aatcttcctg gtctaagtaa agctgggtca agtatatctg gtctatttgt
     1141 agctgccatg attattatac cttgatttac cccaaatcca tccatttcaa ctagaagttg
     1201 attaagagtt tgctctcttt catcatgacc tccaccaaga cctgcacctc tttttctacc
     1261 aactgcatca atttcatcta tgaatatgat agctggagca cttttcttag cctgttcaaa
     1321 taaatctctt actcttgaag caccaacccc aacaaacatc tcaacgaaat cagaaccact
     1381 tatactgaaa aagggaactc ctgcttctcc tgcaactgct cttgatagat atgttttacc
     1441 agttccagga ggtcctacca taagcattcc ttttggtatt cttgcaccca attcaatata
     1501 tttcttagga ttctttaaga aatctactac ctcctgcaaa tcttcttttt cctcatctag
     1561 tccagctaca tctttaaatg taactctagt tttttcatca tctttatgaa ccttagcttt
     1621 tgatttgcca aaactcataa cctttccgcc tccaccttga gactgattca taaacacgaa
     1681 ccaaataatc accatgaaga atattaacag taatgtaggc aacatttcga caaaccaagg
     1741 tgtagatggc ttagcttcac caccaaaagt tagtttccca gccttagctt ggtctaacac
     1801 ctcatctgca agtttatttc ccatcacttc tgttggaaca tatgacttaa actttgtatt
     1861 tgtatctttt atagtacctt ctacagaagt ttgatttaca aagtataatc ttgatatatt
     1921 ctcatctgtt aattctctat atacttcaga gaattttaaa tcttttattt tttctgtagg
     1981 cttacctgag aattgaacta tacctactat aattataaaa acaagtagat aaaaacctgc
     2041 cccttttagc aacttattca atatagaagc cctcctttca tacattgctt tgccctataa
     2101 tttgttaaaa ttaattttta atacttcttt tgtgttttca tcaattttat aattttcact
     2161 gattttataa tctccaacac atattattcc ttcactatct gtgattatag gaattttaca
     2221 tctttcttct cttgggattt ttaaatctat gaataaatct ttcacttttt tacttcccat
     2281 agcaagcttt atcttatcac cttgccttct gcttcttatt actatacccc cttttacctt
     2341 attaaaatca aaccctttag atgaattgtc taatttcata ctcttatacc tatctatgct
     2401 cattacttgg gtctcaataa tttgttttaa ttcttttatt ttaataaacc cattactagg
     2461 tacattgtaa taaaattcaa tttcctcatt aacaatttcc tcattcgtta atattatact
     2521 gtcttttttt ctataaacaa atatatttct tggaagagtt aacattttat ttacttttga
     2581 attatcttct aaagacatta tatcttctat atgtctttgg tcaacaaaat tagtatctcc
     2641 tagtatatgt tttatggaat ttctaagaac tctaactttg atagcatcat gtaagcaaac
     2701 aaaatcgtct aaatttatct ctacaaagcc tttttctttt atatttgaaa cttctctaaa
     2761 tttagcttct gcctctttct caatataatc gttatcactt tttaaactat tgctcatcct
     2821 cactatagat tctattacat ttgaattaaa attatccttc atatatggta aaaggtctaa
     2881 ccttatctta tttctagtgt atatattttc taaatttgtt ttatctatcc tcggatttaa
     2941 attataagct tcacaatact cctctatctc atttctttcc acgtctaata tgggtctaat
     3001 tatgcaatta tctctaatat aatcaattcc ctttaagcct tttaagcctg ttcctctcat
     3061 gatacgcatc ataacagttt cggcttggtc attgagatta tgacctattg ctattttatt
     3121 ggctttaatc ttatccttaa tttcataaaa catttcatat cttagcttcc ttgctccttc
     3181 ttctaatgat aatccctcat tttcacaata ttttggtaca ttaatagact ttacaaagaa
     3241 tataattccc atatcctcac aaagtttaga tacatataat gcatcttttt gagcttctat
     3301 ccctcttatt tgatggttta aatgtgctgc atatatttct atattaaaat ctttttttaa
     3361 tctatttagt acatgcaata aacaaactga atcaggtcct ccagaaagac ctaaaactat
     3421 tttatcgcct ttttgtatta aattatgttt atttatagta cttagtacct tatcaaatat
     3481 catatttatc cctcttacac ttagttttat tcacttttaa gtatactttc tataaaaaaa
     3541 cgctaaaccc ttgtatcaat tttaagaaat taaagtttaa tataaatata ataataccaa
     3601 ataaattaaa taaaaggtag ttttttcact acctttataa aatttctgga aaatagccat
     3661 agttattatt tagtacctat gtatatatct attttattcc tacctaaaaa taaatcttgt
     3721 acctttatat tatagtctat tttcaatttt atgtgttccc cttcttgtat atttatatct
     3781 aattctcttg tatcaatatc aattaataaa gtaccttgtg gggtcacata ctttgtagta
     3841 ctactttctc cacatttaaa cagcattgtt gagttaatag caccaaactt tttgatacta
     3901 acttgatttt taagtatctt tatagtatta gttatttcta tattttcttc tttttccttg
     3961 taaataacat atattccatc atttttataa aaaattttac caaaggcagt catctctatg
     4021 gtgtccatat ttcctttttc atcatattgt ctagtattta tacttatttt tacactcaac
     4081 gatttttcct ccatagattc ttcatataaa ctagtatttt tgtatttcta ctttttgaat
     4141 cttatcaatt tttttcttta agaactctct tgctatatct gaaaattttt caggtatatc
     4201 tgatacataa tattgataag tcccatgaat ttctgtattg tttataatat tttgcacctc
     4261 taaaatcttt tttaaatcct ttgcagtctc tttagctgga ttaacaagtt ttatatgctc
     4321 tccaacttct tcacctattg ttctctttaa aataggataa tgagtacatc ctaacactaa
     4381 agaatctata tttttttctt tcaattcttg taaatatatc tttgctgtca atttagccac
     4441 ttctgtattt gcccatcctt cttcaacaat aggtacaaat aaaggacatg ctttattaac
     4501 aatttctata ctctcatcta ttttagatat ttccaagttg tatgcttttg aacttatagt
     4561 tccttcagtt cctattattc ctacaatttt gttttttgta gaacttacag ctgttctagc
     4621 tcctgcttct ataactccaa ttatcggtat atcatatttt tcttgagctt cttttaagct
     4681 tcttgcagta gcagtgttac atgctataac tatagctttt acaccttttg tttttaaaaa
     4741 gtttatagct tgaaaagtat atttaattat agtttctttg gatcttgaac catatggtat
     4801 tctggcagta tctccaaagt atattatatc ctcatttggt aatatcttca ttatctcttt
     4861 tagaactgtt aatccaccta atcccgaatc aaaaactcct attggtttat tgttcatctt
     4921 tatacaacac ctactttaca tttttgtgtt taacaacaac ttatttatcg ctaaaattta
     4981 tatttatatt cataattaat atattattac ctaaatgatt attatagttt attttacaca
     5041 aaaaataaag gagctatctt aaattagaaa ataatttcta caaaaagaca actcctttca
     5101 ctacttaaaa atttaaattt attttgcaaa cacatgattt cctattctca tataaggatt
     5161 caatctattt atccatttgc aagtggctat ttctggattc caaaaatata ctgctttatt
     5221 agttgggtca gttccataaa gagcttcctg agctgcttta cgtgctgagt ctgttggtgc
     5281 catctttatt gaaccatcaa gcacaactga gaaagcattt ttttgatata tgacttcttc
     5341 tattgtgtct ggaaatctag ggtccaatac tctatttatt actacagctg ctacagcaac
     5401 ctgtccctca tagctttctc ctctagcttc tcctgcaacc aacttagata ataacatcaa
     5461 gtcattatca gataaattta ttacttcttt ctttgctata tttttttctt gtttgtttac
     5521 tttcttaata ttttctgttg gttcatatgc aaatacctct ttaataggca taaatataaa
     5581 agtcaaagct aatagtgtag ccatcagttt actccacctg tccatgatta aaaccactcc
     5641 ttacattctt atttttaaaa ttttttctct aaatccttga ctacttcaag tactttaaac
     5701 ttaattttta ctctacttgt tttttttggt gtatcttgtt ttattaactt atattcttta
     5761 tcagttaaaa cttccttcaa cttatcatct gtagatttat ctatcactcc attagattca
     5821 tcaacaaaac atagtggagg gaacataaca caccaccagt tttttccttc acctttccca
     5881 atgataatct ttaatgcttt atattcacca gctggaagaa ctatattaga atattgttta
     5941 gtagggaaat tgctatattg tatacctacc tttacactgt aattatatcc atttttaaga
     6001 ataatatttt tacttatttc ttccagacta ctatattcct tttttataat agcttcactt
     6061 tcttttatac tttttgaatt ttgaagcttt ggctgtaaat attttattac ttcatctcta
     6121 acttttagtt taagttcttg atcttcatct gtattgctgt ttgctataac atgaaatctt
     6181 attaatttat ctttataatc ttttgatata ttgtctactt tttttacctc accatttatt
     6241 actattgtca ttattgatat gactgatata agacttaata ttaatagtcc caatctaatc
     6301 ttaatttttc tcattgaaaa ttccccctca agataaattt atatcatcaa ttatgtccaa
     6361 ttaaatttaa tttattcata atatttttta atactaataa aaaaagctat cttaaaatga
     6421 ttacaatcat tttaaaatag ctttttaaac cttatatttt ataagtcttt actaatcata
     6481 gccttatcta tcataaactg ctctgctttt tctatatgct ctgtagcata cttcttggat
     6541 agctcactat cacctttaat tatagcatca agtaagtatt tatgctcttc aacaatatta
     6601 tgtgatgtat cataagcaga catataggta actctaaatc tatatacttg ttcccataat
     6661 gagtttatta gttggattaa ttttttatta ttagttgctt taaatataca ttcatgaaat
     6721 tctacatcaa gttttaataa cgcatctaca tcggattcca aagtgctttt tttaaattct
     6781 tctgatatat ctttaagctt tttaatatct tcatcactaa ttctttcagc ggcaagatat
     6841 gctgctaagc cttctaggct tgctcttact tctaatacat ccataatatc ctttaatgac
     6901 atatttgcca catacgcacc ttttctaggc aacataacga ctaaaccttc taattcaagc
     6961 tttcttattg cctctctcac tggagtccta cttactccta attgttctgc tagttgcact
     7021 tccattagcc tttgaccagg ctttaatttt ccttctaaga ttgcttctct taaattctca
     7081 aagactacat ctcttaatgg cttgtaatta tccaagttca gtttagttaa attctccaca
     7141 aatttcaacc cctttttgac tgctattaac aacatacacc tgtttatatt tttttaataa
     7201 ttcttttttt ccaattagag catcttcttt atttttaaat agtccaaaaa ctgtaggacc
     7261 acttccactc atcatagaac ctaaagcatt atttttcatc attacttgtt ttatatcact
     7321 tatttctttg tgtttaccta tagtaacatt ttcaagaata tttaccatgc tttctgatac
     7381 agcttttata tcttcagatt ttaaacactc tatcaaatat ttattatttg gtcttttttt
     7441 aatattattc aaatctaatc cttgataaac ttctttagta gaaacgaata agtctggttt
     7501 gcatatcaat atatttgtat cacatggtaa acctttgatg tttgttagct tttctccaat
     7561 accttgagct aaggcaggtc ttcctgatat acaaaaagga acatctgcgc ccaaattaag
     7621 tcctatttct tttaattcat cttcagataa tctaagttcc cataggtgat ttaaacctac
     7681 taatactgcg gctgcattag aacttcctcc agccatccct gctgctacag gaatattttt
     7741 ttctatgaat atttctacac ctttttttat ataaaattta tttttaagta ttttggcagc
     7801 tttataaaca atattatctt catctaaagg aatatctaaa ctagtgcttt ttacctttat
     7861 ttcatcttcg tctaattctt ttatctttac tatatcgtat aaatctatcg tttgcattat
     7921 catttcaaca aaatgataac cgtcttgcct ttttcccaag acatcaatgg ataaatttat
     7981 ttttgctcta ctctttagtc ttataaaatc cattctatcc tccttgttta aagaataatg
     8041 catcactaac aataatatta tactacaaaa aataaaataa tagggtatta taatacccta
     8101 ttatacttaa ttactttaaa ttttttaata ttaatctact aatacgactt ttttttctaa
     8161 aataagacac ttaccaggct taattggttc atcaagtttt atatcattaa gttctgcaat
     8221 ttcattttct gttgtattgt atttttttgc tatattccag aaagtatctc cttctttgca
     8281 tatatatacg attatgctag gagctttaga taaatcatat acaccttggt cttctccttt
     8341 gataataaaa ttttctgctt tcttatctaa tgcttcagta aatctcttaa ttttgataat
     8401 caagtctatc tggtccctat tcaaatcaac ttcgacttta tcaatacatg ctgtattaaa
     8461 cacagatgct gtatcagtta gattatccat ttctatatca tgttcaaatg gaatttcctc
     8521 actaatttta taaaccaatt ttaaaccttc aactgggaca aataaaattt caacttttat
     8581 gattccttgt ataatgcttt tatttccttc aatataacta ttttctatag acatggttgg
     8641 acaaacgctt acaatatctt ttatttgtat atcatcatta tcatttctta tgccttctct
     8701 aaccataaat gtttcttcac tacgtcttaa agtcttattt aattgaatag gtttgtggtc
     8761 aaattttatt atcttttgag gcgaatatgc atcttgtaat acttctcttg taacttcatc
     8821 tgtaactttt accttacaac aagccataca atctatttct aaaagacctg tgttgctttc
     8881 actattttgc ttgaaaatat gattaaaatc tgacatagac aacaatactt cttcagtcat
     8941 accatcacta actccaggga cttctacaaa ttgagtaaac tcaataccta ctctatccaa
     9001 ctctacaagt tcaccctcat atgtacaagc taacggattt atttctaata ctccaccaat
     9061 aataacttta ttgtcagtca ctctactttc ttttacttta acacatggat ttaaacttat
     9121 tatagattga atttcctctg tgtttattgt tatagtatct cttatagaac tttctgcttt
     9181 ttctattcca actatatctt gaaaacaaat ttcctttctg tgcttttgta tgccttccac
     9241 ttgcgccaca tcttttacaa tgtctaacct ttgcttttca aacaaacttc cccttatatt
     9301 cataagagct cctactttaa tctttctctc attcattata gtacaatcca tatgctctac
     9361 ttctgaaaat aacatgtatt ccatatcttg aacaacattg tctttttcta ttacttcgtt
     9421 tatatctact tttccgtcta cattagaaat agtattttta tcatctgcaa tatagattac
     9481 attataatta aaactacctc tgcaaagtat cttcccatct gccatttcaa tttttttaag
     9541 tgatatgtat ccttctgttt ttacaatttc atatacatct aattttttgt ctggtacaac
     9601 tgcctccgct tcaatgaatg tttgaaattt tccaaagtct attctattgt caactttaat
     9661 tacatcttta attaattcca tagtctacct ccttaagtat tataaaaata tcacactata
     9721 tttatattta aggagcaaaa aaaaaattac acctattttt aaataggtat aattttaact
     9781 tatttgtaat ttatcttgat ctctaaaaac ttgcaacttt acattagatg ttaataaatc
     9841 tgagtaacta taagatactc taggatatcc attactttca tcatctaact ttataacaaa
     9901 cacgcttgga taaacttttt ctagtatacc ctcttttgta ataatctgct ttcttccttt
     9961 attagctttc aacaatattt tctttcctat atgtctctct aagcttactc ttatcttatc
    10021 tagagtttga acagtagcca caatatcacc ttctttaacg tttatatgtt tacataatat
    10081 cacatataaa catttttgtc aaatgtttaa actaattatt ttatatattt tttgcaaaaa
    10141 tgtcaatact atttttgaag atatacagta taattatgtt aatttttgct aatgtaatat
    10201 tgtttttggc attgcccatc taaattttcc tcttgtttca gaaccaccaa ttcccttaac
    10261 acctgtaata gtctgctcta taacctcatc aataggaact actacatcaa ttcttgaaca
    10321 agcaattttc tccactataa aaacagggtc taatgcatgt atcattattc ttccaggcga
    10381 acttgcataa tttgctcctg cacttattat tttttcataa tttgattggc atgctcctgc
    10441 gaatatgaca agattatcca aatttggttg ccatttacgt gcttctttta ctgttttaac
    10501 aaaatttgaa gagtttctat agttactcat atcttctata tctcctcttt ttattgtcat
    10561 tgcatcatga cctgttatta ctagtatatc cggattatat ttttctagta gtgctcttac
    10621 ttctttatac tgattttgct ctgaaacagc tacacctact gcaggtattc caagttgagc
    10681 atatacatcc aagcaaattt ttaaatattc tttatctcca tctatctgta gtacttttcc
    10741 aggcattcca tatgtgttag gatttaattg aagttttgga actgctctag tcattctttt
    10801 ttgtctttct ttagcttttc ttactgactt atataataaa ttttctacat ttttatctat
    10861 taatatatct tttatatcag gtgcttttac taattctagg tcatcaatat aagcatctgc
    10921 tattactcta aatgcaattc cctttagtat agctattttc tcattatttt catctacacc
    10981 aaaagagact attttaaata ctatatcttt gttatgtgac tttctagcta caatgtctcc
    11041 taccttcata gttaaacctc caataaattt ttatcttagt ttatactatg ttcgatataa
    11101 aactttgttt cctaatgtca aaaaaacatt tcttataata cttcttttat cactcttaaa
    11161 gcatttttat agtatatctt ttctatatca tcttcactaa acccttcttt ttttagaggt
    11221 tcatacagtt tacccatctg agatatatct tcaatctcaa cttttgaatc tattccatca
    11281 aaatctgacc ctagggatac aacatctatt cctccaacat ttacaatatg ttttatgtgt
    11341 ctaaccatat cttcaagttt actttcactt ttatcactta aaaataagtg gaagaagttt
    11401 atacctgtaa ctccaccttt atttgctaat actttaatca tatcatcagt taaatttcta
    11461 gaatgattca tcattgctct tgaattagaa tgtgtagcaa ttattggttt agaagaaatc
    11521 tttgcaattt cataaaatcc accatctgaa atatgagaaa catcaaccaa cattcccaat
    11581 tcattcatct tatgtaccac ttctcttcca aaatcagtaa gccctttttc tctatattct
    11641 actttattat gaggaaagct caattcattc acatgattcc aacttattgt catcattcta
    11701 acccctaagt cgtaaaattt ctttaaattt tctaatttac cttcaagtac tgcaccttct
    11761 tcaatagata aaagagctgt aagtttacct tctgattggt ttttcataat ttcttcatag
    11821 ttagtagcta aagctatttc atcactattt tttttcattt cctcatgaaa tttatttgcc
    11881 atactcatgc aataatcaaa aggatgtttt acttcttcag tatcaacaaa aagagcaaat
    11941 gtttgagcta atgaatcccc ctttttcaat ctgtctatat caacagaata tttattagac
    12001 ttaagttcac ttgtttctac attttccatt aattttgcta tagtatcaca atgtaaatca
    12061 ataaatttca tatttatacc ccctgtatat tttatattta tatctaaaaa tagaaatagt
    12121 tgtccttata gacaactatt gacaactatt ttttaatatt ctttttaata ttacttaatc
    12181 ttattcttct tttaagttag gtattacttt tttaacttct tcaatttgat gccatgtttt
    12241 atctatatta ttttcactaa tatatttttt taaatcagtt aaagatgtaa aatctttaac
    12301 tgataaattg tcatcatcat ctgcaaatct taaaataaac atgagactcc cttactttcc
    12361 acaacatttc ttatattttt ttccgcttcc acatgggcat gaatcatttc ttcctatttt
    12421 tgcttcttta actattgttt tagacttctt ataagccttt attatttctt ttctttggtc
    12481 tgctggtagt atatcttccc aaccttgtaa attatatagc caatgagcct caacagttac
    12541 catattgtta tataattttt cccaatctat ttttatagaa acttctttat cagcttctac
    12601 ttcttctaaa actatatctt cttttaagct ttcacttatt ccatctatga atcccatgaa
    12661 aaattcatta gtagttccaa atttttcagc caattcactt actttacctt caacaacatc
    12721 cattttatta tttaatattt cattgtatat tccagcttca actttcaaat attcttccca
    12781 gaattttact tctgcttctt gactttcatg attatcactt aaatttctcc attcagtata
    12841 taaactcata ctttttcctc ctaagtatac ctatattttt atagttttta tagtataaaa
    12901 tcttttagtt agattggaag tttcttcttt ttgattatac catattctat ttaaagttaa
    12961 ctcttatatt atatcataat taaacaattc ttttaatact tttatagcat actcttggtt
    13021 tataaaataa gggcttgtcg caattgttat tactttggct tttttaatta agttttttat
    13081 cttgtaaaat ttgagttcaa atggtatata gtccatatca cttgaaaata tatccaaatc
    13141 tatatcaaga acaaactctg attctacttc taaatcaaat ccatacagac tatctattat
    13201 tattaattct gaaaatatgt tatattttaa agcaggtttt ataaaacttc ccacatttaa
    13261 aacttcattt gtatacctaa atacatcttt tagattattt acatctacat catagttctc
    13321 aggctctcta gtatccttat gttggtctac atgcactaac ttgcatcctt ttgtaaattc
    13381 accctttaac aaagattttg cccaaaaata aaatgcatgg ttatgattgt caaacacata
    13441 tatcttttta tttcccagga catattcgac catattctcc aatccttttg cttttatttc
    13501 cttattttca tctacttcat taaataccac ctcattgcct aaccttacat catttaatgt
    13561 accatttata agttttggaa cgtatatgct cttattttct cttttatcat atgaaaatac
    13621 attatttcct actggttttt ctatataaaa tccactatat tcataatttt ccataaaact
    13681 ttatttcctc cattgttata taaatatata agttataatt ttaaaaatta aaatgcactt
    13741 attgtaaatc atattatcta ttatagaaaa catcttatat aactaaatat aatatcttct
    13801 ctcaatatgt attatacaat aagtgcaaaa tatatttata taaaatatac tacattccta
    13861 taacaaaacc atcagcttca attggaaggt aactaactct tctattcaac tccacctttc
    13921 caaaaaatac tacccaatat ggtctaaaaa tactttcaat tttaactatt tcaatctctg
    13981 ggattcctcc cataaaccta tgaattaatc tcatagccat tttatttgcc tttgctttta
    14041 ctcttcgttc gtccttatta ctatactgca caatattaca atccacattt tttacaattt
    14101 catatgtagg aaaatcttgt actatactac cagaacctgt agaaccattc actaaaattt
    14161 ttaattcatt tatatcatta gaatatctcc tattcttgaa aatggatttc gttttgtgaa
    14221 ttttaagtgt gataatttta aactctatat aatgaagtct aacttcatga gcatatttgt
    14281 tctttaatat tagcttacta aataaagaat ttttcccact tttagctatc tctatagctt
    14341 cttcttttgt aattcttata ggtaacgctc ttatatccat aaaatcattc tctttcttat
    14401 ttttttcttg tcatttttaa aactctatat atataatcaa aaaatattac aattacaact
    14461 gccataactc catatggaat tatttttcca actataatat catttctagt caatatacca
    14521 taaaatataa atcctgttat taaaaagtac aataatacta gaaaaccgtc tactccaagc
    14581 cattttattt ctaaatcttt ttttacctta gacactaatt tagacatagc agtttctcct
    14641 tttatagatt attttgtgta tttaaatttt cacctattct attaccaatc atccatgcaa
    14701 taaatgaaaa aggtatagct atcacaagag gctctaactc aaagctctga actggaaata
    14761 ttttaaaata accaaaagtc actccaccta caatcattcc taaaatacca cccatagatg
    14821 ttcttcttcc atttccaaga agcccaccta ctataggtac aaatgccgaa cccatccaaa
    14881 tacttcctac aaatatacat gcatcatata cattttcaaa tctaaatgct acgctaagcc
    14941 ccataactgc tgctaaacat acacctattc tagtaaactt tactaattgt ttctgagata
    15001 actctttttt tgtaattctt ccatatatat catttgctaa tgtagctcca gctgctagat
    15061 aatagctatc caatgttgat attatagaac ctaaaattcc tattataaat attactctta
    15121 ttccttgtgg taatgtatct aaaactaaag ttatatatcc agcggctgga gccatctctg
    15181 gataagtagc tcttactata agtcctgtta tagttaacac catgtcaaaa catatccata
    15241 gtgaaaaaca agttaataag gctcttcttc caaccttagg agaatctgct gctgaaaatc
    15301 tttgataaaa agttgggtct gaataagctc caaaagaata tacaattaac atcaatgctt
    15361 tcatagctga catacctttt gtagggtgca taagctgtgg tgcatctttg agtgcttctg
    15421 tgagaccttg aaatcctcct atttgttcaa attgacctgg cataaccata gttaaaccaa
    15481 ataccataca aaaaaacaat accatatctg taatggcaac tcccataagc ccaccaagta
    15541 ctgtaagtac aacgactact cctaccatta ttaatcctat taattcttta tctattttcc
    15601 atgctacgtt acctatagtt cccatagcag ttatttctcc aagttgtgca caatatataa
    15661 acatcaaaat tgccccaatt atagcaacat gcttaccata aaaactttcc agtaaatcag
    15721 gaactgttat attggttctt atatgaatcc ttggaccaac ccacaatgct aaaggcattc
    15781 tcccaatatg tgcagctatt gaccaaactg cccatgcaga tattccatat atagccgcat
    15841 actctacact acctactgta ccaactcctc cataccatga tgcaaccaaa gttcctacta
    15901 ttaaggacca tggtaaactt cgatttccta ggtaataact ttccatatct tttacatttc
    15961 tagaaaaata ataacctatt cctaataata taaatgcaaa tataactata atcccatttt
    16021 cccaaaatgt taacatatct aatctccaca ttcttatatt ttaattttta actttaatat
    16081 ctaaatatga acaactctac ccatttccat aaaaataggt agagttatat aataattata
    16141 agtatttaag taatcttgtt attaagcaat tttattgttt aaacctttat tttctctttt
    16201 atttatttta tttaaagcat aagtagcaaa tatccctata actgcacata ctacagataa
    16261 aataaatata tatgtatatc ctgtttgtcc atatttatct aaccaattac ctgcaatagt
    16321 gtaataaaac gcatctggag aaaatcctat aaatgaagca aatccaacta ctgctcctgt
    16381 agtttctaaa gatatattag actctgaaac agatgcaaag tatatacctc taaatccaaa
    16441 taaaattaca cttagtatta tcatgtttat tacagctatc catataaatc ctgtactctt
    16501 aggaataacc aagtaagcag ctgtacttac tgtcattaat ataaatccta caaacattac
    16561 tcttatagat gaaccaactt tatctgttat aacaccagca actggagatg ctcccatttt
    16621 tataacataa gttcttatta ttgataaagc agaaactaga gtcattggca ttacataaac
    16681 ttctgataag tatggactta aataagttaa acttgagaat actaaataag ttgaaaatat
    16741 tataagacca attaaccaag cctttggcat tgtaactact tttaacaatg acttaatact
    16801 taatccttca tctgatgctc cactatcttt tttctcttcc actaagaaaa atagaagtat
    16861 accacatatt atagatgctg cagaatataa ccatactaca tatttgaatc ctactgttat
    16921 ttcagcaaat ttaccaaaga agtaaagacc tataaaagaa attaacgtac ctgatacacc
    16981 tgataaccct tcataaaaac caaatattct tccttgttct gactcacttc cttgcattct
    17041 aacaacctta acagatgctg agaagaaagt taatattgtt gtaaatcccc ataatacaaa
    17101 tattaagagt atcatactat atgatgggaa tgtagagaaa taaaaaccaa gtactcctga
    17161 agcaattaaa gaaaaagtta tcagctttct aaccgagaat ctatctgcaa accatcctcc
    17221 tataaaataa gataccattc ctactaatcc atataaactc aataagttcc ctatttgtgt
    17281 atggctcaat gataaagctt gttgcattgg gtcataaaat gatgatttca tatatggtaa
    17341 actatacata gccgttgttc caaatgccaa tatgaatatt atcaagaatc ttttaaaatt
    17401 acttttgttc tccattaatt ttctctcctt aaaattttat tgtttataat ctcattcatg
    17461 taattttatt ttaatttttt tattgcttgt ttaaagtcat ttattatatc atttgaagat
    17521 tctaatccta cagctattct tattagattt tctccattca tttgaactaa tgtatgctct
    17581 tcccctaagc ttgttgctat tgttgcaagc tctaaagagt tcattaattt tctactagct
    17641 tggtctcttg tcaatccatt gataccatcc ttaagtttaa atgaaactac acctccacct
    17701 aaaccattca tttgagtttt acaaagctca tattgaggat gagattttaa tgctggatac
    17761 tttaccaatt ccacattctc ttcttcctct aaaaactctg ctactgctaa tccattttca
    17821 caatgctttt tcatcctcat atctaacgtt ttcatacctc ttaaaatcat ccatgcactc
    17881 attggtggta aagatgctcc tgtaaaacaa cataaacttg gccatctaac taaatctatt
    17941 aattcttttg aacctgctac tgctccccct aaagtatccc catgtccatt tataaactta
    18001 gtaagactat gtattactaa atctgcacct aaatctattg gtctttgtac aattggtgtc
    18061 ccaaaagtac tatcaactat agctaagctt ttattttcat gagcaatttc tgctattgtt
    18121 tttatatcta ctaagtctaa tgatggattt aaaggacttt ctatataaac tattttagta
    18181 tttggtctca ttgcttcttt tacattttgt ggatttactg catctacaaa tgtaacttca
    18241 actccaaatt tagataacaa ctcagacatg aataatttag tgtgtgaaaa tattccatct
    18301 gaactaacta tatggtcacc actttttacc aatgctaata tagtagatgt tattgccccc
    18361 atccctgatg tactagatat ggcactttct gcattttcaa gcatagccaa cttagcttct
    18421 aactctgtaa gagtaggatt acactctctt gtatacacat atccccatgt catacacaac
    18481 tcttcaaaat gctctactgt ctttgcagca aatgttgctg tttgacatat tggtgtagcc
    18541 aatgcacctg ttgttgggtc tgcactatgt ccccaatgta ttatttttgt ctctaaatct
    18601 tgattaatat tgttactcat tttgatttcc tcccattttg acttatttat acaacgtttt
    18661 ccctgccaaa tttaatcaat ttagcttagt cttaatacat tttttcaagt atactttttt
    18721 atttgttata atttctaaaa atatgacact tattttgttt ttttacagtt ctaattataa
    18781 actctatagt aactatagag tcaatatgtt tttgatattt tttttacaat gtttaaaata
    18841 gtcagtacaa aaataggtat atcttagaat aactaaagtc atcctaagat atacctattt
    18901 ttatatcaca atataaataa ttacatataa gcattagttt ttttatcata aatagatttt
    18961 tcaagattta tcatcttttc ataaatatat tatagttaaa tattttaata ttacctaaca
    19021 tctacaataa aatatactct taaaataaaa ttatataatt aaaaatactc tataaaagct
    19081 aaaaagtttt ctattcgcta catctaaaaa taattagata gaggtataaa tgtatttagt
    19141 ttatttatac ctctagtctt ttataaatta tatctctact aaattgattt tttagcacga
    19201 ataagtgtgc tcttggagat accagtcatc ttcacaacct ctctatatgg cttagaattt
    19261 aataacccca atgcatattg tatctgttga ttgctgtatt tctttggcct accatcttta
    19321 aagtcctttt tctttttagc aaattctttt cctaattgag tacgttctat tatcatattt
    19381 ctctcaagct ctgcaactgc aagtaatgta gtcaaaaaga atttacccat tgaagtattt
    19441 tctaaaagac ctatatttaa aatatgaaca cttacttctt tagcaaataa accttctatt
    19501 agttgtatcc cttctgatgt acttctagct agtctatcca gcttagttgc aacaagtaag
    19561 tctccctttt ttagagatga tataattttt tcaaatttag gcctgtttgt agtagtgcct
    19621 gtataagttt cctttattat tttagcatca ttatatttat tcaaaatttc ttgctgttgc
    19681 tgatacaaac tgttgttgtc tgtttgattt tttgtactta cacggcaata tccatatata
    19741 ataatcatca cttcctaaca taaagatttt gtacagaagt tacccagaag cttgttttta
    19801 cttatcttta tccattttac taaaacgatt tttgggtgtc aatactgtta acttttgaca
    19861 ccataataat tcctttataa tttgtttgac tatgtcaaaa attaccaata taatgaaatt
    19921 aatgagttta tacaaattgg aggaaataat atgaatagtc tgttaaaaga agcaatagat
    19981 gaattaaaaa acatccattg cggggaagtt tttttattaa aagaattgtt caaaggctat
    20041 gaatggaaca ggttggagat gaatattaga ttgaaattag gaaaactttt tttgtatgaa
    20101 gtggtaacta atcccaattt aaatgtagag gtcatcaata aaaattctgc ttatcagcaa
    20161 atgtataaaa aaaaatagtg acttaaatgt atataatagt ataatcttct tattgattca
    20221 aaagttaaaa cttttaatac aaaacaatca ttctatttta acatttatca atatgtctaa
    20281 atttatataa ttaacacata cgaatagtat tgtttaaatc acattattta agatagataa
    20341 atcttaaccc tactccagca taaatactaa aatagggctt agaattatct atatttttat
    20401 cccataaaat gcttagctct tcctcttgat tgaacatata tatctgggga aatacaatag
    20461 aagcgtaaat ttataaaatt tccaaagtca gaaaacttca gttcaaactt aggtatacca
    20521 aaactttcag gtgctatttc atcaacgcca aaatagatga taattccatt atctgtaata
    20581 taaaatgctt ggtcttcagg tataactaac tcaacattag aataatacat ttctctattt
    20641 tcatttattt gagatttaat gtaatcagta attatattaa tataatcaac tccatctcta
    20701 aaaatgtcat taagtaaaag ttcttttcca gttgtcaaat ctatattgta attattcaat
    20761 gtattgtatt taggcccata gttattagca aaccccataa ggtttaatat catactcagt
    20821 acataatttt tattaaaagt aactgcgtaa ctggtagata cccaatagct ttgttttgga
    20881 agtgaatttt gagaagctgc gttattatat tctcgttctt cttcctgtaa gccattttta
    20941 aaattattta cagtcattct tacagcattg tttatgttat ttaaaataac tgggtttaca
    21001 tacgtggatt gatttctaat attgtatata acaggatact ttacatcata tgaaaaatac
    21061 ttacttcttt caaaatcttc attaaattgt acatataata tattatatgt acaggtattt
    21121 gtagattgat aattttccat taaaatcctc ccccttttat aaaaaaatca gatacaatat
    21181 aatatgctta aaacagtaat aatgttaata gtaaatgtaa agaatagaaa gtatctaaaa
    21241 cagcaaaaaa tgactaattt tgtggtataa tacataggat tgagagttat tttataggag
    21301 gaaaaatatg ttattattag cagataaatg gaaagattat gaacttatag atatgggaaa
    21361 tggagaaaaa ttagaacgtt ggggaagtta tgtacttaga agaccagacc cacaggttgt
    21421 atggccaatg gaatctgaat gggctttatg gaaaaaccct catggacatt atcacagaag
    21481 caataaaggt ggaggtcaat gggaacacaa aaaaagatat ccagaacaat ggactataaa
    21541 ttataaaaac ttaaagtttc acattaaacc aactggcttt aaacatactg gattattccc
    21601 agagcaagca gctaactggg attggatgat agagaaaata aagaaagcta acagacctat
    21661 aaaagtgttg aatctttttg catatacagg tggagctact gtagcatgtg catctgctgg
    21721 tgcagaagta tgtcatgttg atgctgcaaa aggtatggtt aattgggcaa aagaaaatat
    21781 agaattatca ggtcttaaaa atcaaacagt aagatttata gtggatgatg ttgtaaagtt
    21841 tgttgaaaga gaaataagaa gaggtaaaaa atatgatgca ataataatgg accctccttc
    21901 ttatggaaga ggtcctaaag gagaagtatg gcaaatagaa gaaaagttat acagttttgt
    21961 agatttatgt atgggagttt tatcagataa ccctttattt ttcttaatta attcttatac
    22021 aacaggattt tctccaatag ttcttgaaaa tgtattgtct acaacaatag gtaaaaagac
    22081 aaatacagga gatatatacg gtggggaatt aggaatacca gcttccagag atggtaagat
    22141 acttccttgt ggtatatttg gaagatggga gaaaaaataa tgataaaagt catatatgaa
    22201 gataaccatt tattagttgt agaaaaacca gttaacatat tgtctcaagg agataatact
    22261 aatgataaag atatggtaaa tcttttaaaa gactatatta aaaaaaagta taacaaacct
    22321 ggaaatgtat atattggttt aatacataga ttggatagac cagtaggtgg agttatggtg
    22381 tttgcaaaaa catcaaaagc agcatctaga ttgtctgagc aggtaagaag taagactttc
    22441 aaaaaaactt atctagctgt tgttcatggc aagataaaat ctaactcaga tatactaaaa
    22501 gattttttat ataaaaataa aaaaactaat atggtaactg tagtgaaaaa aagccataaa
    22561 gatgccaaag aagctgaact taactataat gtattagatt ttaaagaaaa tctaagttta
    22621 gttcaagtta atttaaagac tggtcgttct catcaaataa gagttcaatt tgcaagcaga
    22681 aaacatccat tatacggaga ccaaagatat ggcaaagaaa taaataagat tggacaacaa
    22741 atatctcttt ggtccaataa gatagaaatt tgccatccta ctacaaaaga aaaaatggaa
    22801 tttacatgtc atccacctga caaatatcca tggaatatat tttaaatctc aaatgaggtg
    22861 attatatgag taaaaaattt tcaataggag aaatgtctaa acttcataaa ataagcatac
    22921 agacactaag gtactatgac caaatagatt tatttaaacc tgttcatgtt gaccaagaaa
    22981 gcaattacag atattactca attgaccaat tttcacaatt agatattata aaatatttaa
    23041 aatatttagg tatgccttta aaagaaatta agtcaaagct tgatgaaaat aatgaagaaa
    23101 ttctaaaatt actaacaaat aaaattaata tagtggatga aaaaattaaa gaattagagc
    23161 ttataaagaa ggtactaagc acaaagaaag aaactatgga aaaaggtatg aaagtagatg
    23221 aagttggtaa aatagtgaga aaacatattc cacgaagaag tatcttatct atagagtatg
    23281 aagaaggatt aagtagtgaa gaaactatgg aactttccag aagacaaata gcaaatatat
    23341 tagaagataa tatatctgtg ttctatggtg gagtaagtgg acttgtatct atagataaat
    23401 ttttaaaaga agataaagtt atttatgaca atagttttgt tactgttgaa agagacttat
    23461 ttgatataaa atcacaaaaa catataacag aaatttcatc tggtgatttt atttgtatga
    23521 catataaagg gtcttacaaa gaaaattata ccgcttttaa gaaactggtt gattatgttg
    23581 aatataaaag aatacctatt gaaaattata tatatgaaat acctataata gaccctctgt
    23641 ctacaaacaa cgaagaggaa cttctaacag aaattcaaat acaagttaaa aagagagaaa
    23701 ttgacttaag atgtctagtg tagtaatcta gtttacttac acatcttaat atagttgtct
    23761 attatcaaca tatgattttt ctagatagtg attcctaaca gtgagatttt caaaaaatag
    23821 gaggaaatat ggctaatgct aattccctcc tatttttaat taatccatat cattttaatt
    23881 aagccatgtc atcacgaagt gcatcaataa tattttcttt ttttatctta ttaatagtat
    23941 acaacattgt aataaatact ataaataaaa cactaaatat actaattcca atactaaacc
    24001 atggaatcac aaaatcaata tcatctgtaa ccattacttt atgaattagc catgaagaaa
    24061 ttactgctat aggaagtcca aagaacaatg ctctcatgcc ataaaaagca cactcaaaat
    24121 tcatcatctt ttggaaatca tgctcagaca ttcctacaga gcgaagcatg gcaagttctc
    24181 tacgacgtaa cttaatatta gttgaaattg tattaaacac attggcaatc gaaatcagag
    24241 aaatcataat tatgaaagta taggcaaaaa cattggcaat aaaaatgtaa ttacggttct
    24301 catccatcat tccatacata ttgtacagat tgtactcatt agtaactccc tcactctcaa
    24361 taatttgctc catttctgca actgactgag taggattttt cgattgaaag cttaaacttt
    24421 caattcctgt agaaacacca ggaatctcaa acttatcttt ggctgaataa ggagccaata
    24481 ctctgaagct tgctttcttc acatctgaac ttctgattcc tggaggagaa tcaggtggca
    24541 ctactttgac aaatgaaata ttcacatttt gtctctcagg tatctccaac ttgccattag
    24601 tttcaggagt gagactaaca ttcatagaag aactgttgaa aatatcaata atattattat
    24661 cctttgttac catagaaaca gcaatcagtt ttgcattttg tccagtatat tcttctgctg
    24721 gtaatccaag acttttaata atattcagat aagttctatc atcaagaaat tgcatatcta
    24781 ctggtaattc aagcatttca ctcatagaat gtgaaccctc atagttcaaa taatcgtctg
    24841 taagctcact tactttggca acacatgaat aggtcataag tttctgatac aaaccttcgt
    24901 aaacaccatt tgcagttttt agcttatcgt aaagcttcaa catttcgttg tctttcatac
    24961 cttgtgtaga aaaaataata tcctgatcag taacctctga cctctgattt gacatctgtt
    25021 ttaggtctgc tactaaagca cttgtagata taaataaaac tatgcttaaa acaagcgaca
    25081 atacaatact tcgataacgt ttcttgtttc ttttaaagtt tttcatagca agaactccct
    25141 caagaccata aactcgctgt gttagttttg atgtcttcac agatttagat tcaattttta
    25201 cctcatttgt ctgacgaata cactccatca ctggtgtgtt ggcagccttt cttgctggta
    25261 tgtaagcaga aattaagatt gtaaccatac ttaccactgc tgcaacgatg attgcaggga
    25321 tagacaatgt taatgtcaac ggaacgttta catagagaat atttgcaaag tttttagcta
    25381 tgacagaaat cacaagtcca atactgacta taccgattgc aacaccaatt ggtataccaa
    25441 tcgcaccaat gcaaattcct tcaaatagca ccgaatttcg taactgtttt gctgtagctc
    25501 caacagatga aagtattcca aattgatgtg ttcgctcgtt caatgacatg ttaaatgaat
    25561 tatatattag aaaaattgag cctagcatga ttatggcaag cacaatgcca ccaaccgagt
    25621 acaaaaacat attgaacaaa ttgttatcag aaacacctat gaagcgcaac acattgtcat
    25681 tgaaaatgga agcatggtct ttagtgctac ttgtgtaaga gcgaatctga tgtggatttt
    25741 tcaatgtgac aaaaaggcta agactgtcag tcttgacttg tttatcttct ttggttatca
    25801 aggtatatcc tggtgcagaa gattcttcaa atacgggctt tctacagata cccacaactg
    25861 tataagtttt cttatcctgt ggcacaaaaa cttcactccc agcagtgtag gggtcactct
    25921 gaccaagctt ttcattttca ctcattcgtt tccctacatc caaagaaata gtatcaccta
    25981 ctgcaaattt gacaccacca tctgtcgcaa ctttacttga aacaagaatc tctctactat
    26041 tttcaggtaa tcttccagaa attaaagtta taggcaaagt atcaaaagct tctttgctaa
    26101 atccagcaat aaaaagatat ggtttatctg gatttctgtt accttcaagt gttgcatagc
    26161 caatattttc aaatgttgca gtatttgcaa cttcatcatt gactctttgt tcctgtacaa
    26221 aagaagaatc cacatccaaa aactggacat gccaatcacc atattttata atagctccat
    26281 ttgtcatata gttcatcaaa gaaactccaa aggtagtaac tgctgtaatc atagcagctg
    26341 acagaacaac tcctataatt gttacaagag ttcgagtgcg attctttttc aatccttgca
    26401 gagcaacttt gttaaaaatg ttcattaacg attcaccttc ctctcatcac gcaccacttt
    26461 tccatctgaa atacagataa ttctatctgc ctgtaaagca atgttttcat catgtgtgac
    26521 aataatgaga gtttggtgat atttactatg gctctctttc agtaactgga taatctcttg
    26581 tccattttta ctgtctaagc tacctgtagg ctcatcagca agcataactg ctggtgcatt
    26641 catcaatgca cgaccaatgg caacacgttg ctgttggccc ccagaaagtt gatttggtaa
    26701 atgtgttttt cgttctttta aaccaagtaa ttctaacaag tcatttaacc gttcttgatt
    26761 gaccttacgc ttatccataa gtataggcaa tgtgatattt tccactacat ttaatgtggg
    26821 tatcagattg tgaaactgat aaatcaatcc aacttgtcgc ctacgaaaaa tagcaagttt
    26881 ttcattgctt tggccatata catcttgccc ctccagatat atctttccac ttgttggtac
    26941 gtctactcct gcaatggaat gtagtagtgt agatttacca gaaccagaag aaccaatgat
    27001 tgcagtaaac tccccttttt caatagtaag tgagacatgg tcaagtgcag taaccttatt
    27061 ttcattctta ccataaacct tacataaatc ttcaatcttt aaaaacgcca ttatataagt
    27121 ctcctttcat attttataaa tatatcatag aactttcaac ttacatttaa gtgacattta
    27181 aatgagagat tcgtcacttt ggaaaacgaa taacaaatat tgcaccacct tgtgggtgat
    27241 tttgtgcagt aattgttcct ccttgtctca taataatcgt cttacaaagt gccaatccta
    27301 ttccataacc agctgtgctt gaatttttcc cacgataaaa tctgttaaat agacatggta
    27361 agtcttcttt tttaaagcct gctccgttgt catgaatagt aagttgagta aacaaaaagt
    27421 tgtcctcaca aacgatatca atttttccat tatcacccac actctccatg cagtttttaa
    27481 atatattttg aattgcttct gaaagccaat ttaaatcacc caaaatcctt actccttttg
    27541 gtatatcaat atgtaaagtg atattgtgca gttccatggg aatcaaaagt tgatgaagtg
    27601 cagaactaat caaattattc acatcaattt gttctttttg aaagactaca atacctgcat
    27661 ctaaacgtga taattttaaa agggaagtaa tcagccagtc cattcgtata agtaattcct
    27721 ctgtttctcg tatcaactct tttctctcat cttcatcaga agtattctct aacagtgaca
    27781 aaataagagt tactgatgta agaggagtgc gaagctggtg ggctatgtca gctagtgagt
    27841 cagcaaggtg ttctttttct tttttcagtg cgtaattttg ctctctaata cgtaacgtca
    27901 ttttagttat ttcactttgt agaatagaaa gctcaccctc atctgattca gcaatataca
    27961 gatggttagc attatgaagc acaagattaa tttgttctga aatctgtgca atacttttgt
    28021 atcgattttt agtaaacaca aaaaatgcta ttccaaaggt agtggcagaa acaatagaaa
    28081 gtattccagt cataatactg attgcaaatc ccagggctac agtgagaata gtaattaata
    28141 agaataaaat agcaaatttt cgaaactctc tatttcgaaa cataacttcc ccccaatcta
    28201 taccctgtcc cacgaacagt caaaattatt tgtggacttg aagggtcatc ttctatcttc
    28261 tctcgcaagc gtttgatgta cacagtcaat gtgttgtcat taacaaactc acctgccgca
    28321 tcccacaatt catcaagtaa tcttaccctc gtaataatac ttttaggatt gctaataaac
    28381 accaataaca aacggtattc taaagctgaa agaaaaacct cattgccatt ctttttcaca
    28441 acaccggttg ccgtatctac ataaagccca caaatttcaa aagttgaccc aaaacgccca
    28501 ctttttcgta gtgctgttct aattcgcgca atcagttcac gtggacgaaa aggtttagta
    28561 atatagtcat ctgcacccat attcagtcca gtaacaacac ttgattcatc accagaagcc
    28621 gtcaaaaaga taacaggaat gtcttgcttt tctttgattt ctgtgcaaat cgtaaaccca
    28681 tttccatcag gtaaagaaat atcaataagt gccaaatcaa atttattctt agcaatctct
    28741 acaagtgctt cacttcttgt ggatacatgg gttactataa atccttctga attaagcaaa
    28801 agaataagat ttttggcaat tgctttatca tcctcaacta aaaatatccg tttcatttac
    28861 tttcatcctc ctttatttta ttagttttat atattccagc agagcaatag tacactacta
    28921 ttttacttat cattacacaa ttttcagtag tttcttttgt tcttatataa tccatgcata
    28981 atacctccat tgatttatta tatttcttat aacataaata cacaagataa gacaaaaaac
    29041 ataatgtaag tgcaagtttt ttaactcact aacttataaa catacactcc tactaacact
    29101 actttcttga tgacacatct tacaagctta tattcataca tcaatgaaaa attaaattta
    29161 tccatttatt aaaataaaag gcaatagctt ctatagctaa tgccctttat cttaaaatga
    29221 tttaaatact atacaatttt tataaatggc tataaataaa ctcgtataga atataaaatt
    29281 tatctacagt cttttgttat acaaaattta taacctctta tagaacaaat ttaccacttt
    29341 gtaaacttat cacttttact cagctatatt ataaactttt aatttatctt tgtttaaatg
    29401 tttcttctta acttctacta aagcttttag cgtatccaaa gatgtcatta tttcagtaga
    29461 aaattcaatg gctgttcttc tcatcttaaa tccatctctt gtagagtcat ttcctttagt
    29521 tggtgtattt attactatat caacttgttt atttcttata gcatctaata tgtttggtct
    29581 aggctcttca attc
//
LOCUS       DAMJWP010000022        28922 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.023, whole genome shotgun sequence.
ACCESSION   DAMJWP010000022 DAMJWP010000000
VERSION     DAMJWP010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28922
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.023"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            27..233
                     /locus_tag="PC700_000906"
     CDS             27..233
                     /locus_tag="PC700_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580229.1"
                     /translation="MSKYSSLWEYVKKNNSQSFKLTFEEIKDIAGIEIDHSFLKYKKE
                     LNEYGYQVGKISLKEKTVIFNKID"
     gene            complement(544..1287)
                     /locus_tag="PC700_000907"
     CDS             complement(544..1287)
                     /locus_tag="PC700_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HDF2580230.1"
                     /translation="MNFLSDLHTHSIVSGHGYSTLLENINYCKENGIKILGTSEHGPK
                     MPGSPHRWYFHNIQNIPRIINNIIILRGCEANILDIKGNIDLEPFVIPRLDYLILSFH
                     EAVFSPNTLENNTKALINAINKHDNIEILGHLGNPNYPIDYELIIKLAIEKNILIEIN
                     NCSIKGVSRNGSSDNCKYIATLCKKYGAKIILTSDAHICFDIGNYEYSENILKEINFP
                     DELIMNYPKKLINHFHQKGKLLDVDYTNI"
     gene            1453..4020
                     /locus_tag="PC700_000908"
     CDS             1453..4020
                     /locus_tag="PC700_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2580231.1"
                     /translation="MTQKKLSRKEKVLICLKRLTEEHLNFNNGIRFGFDAEYIATEVG
                     INRSNASKELNQLIKESIIIKIKGKPVQYLYKESIESILGKKLNKGIFDNINSINEYS
                     ENDSIKDEYILESIKKENNIFNLVGCDGSLRSQIEQAKAAVIYPPKGLSTLIIGPTGV
                     GKSIFAEYMYKYSLTIKEDSDNAPFIIFNCADYSDNQQLLLAQLFGYVKGAFTGADKD
                     KYGIVHEANNGVLFLDEVHRLSAEGQEMLFLLMDKGIYRRLGEANKVHECRVMIIAAT
                     TEDPETAMLETFLRRIPVTIKIPSLEERGFQERMKLICYFFKEESVRIGVKLKVSKEV
                     IKAFILYKCKGNIGQLKSDIQLICARAFLDYMTYKRECVYVRLSLLPNNIRESLYGNN
                     RKEEFVQNFSFIGKDDIIFLPEEKDFESENLLIENKKYDLDFYGMIKETWNKLQQEGI
                     KESQIRSVIDGDIQKYSYNLMNTFIYNNSNQTAYNNIVNDSIATTVKYILQKHDKWSK
                     REGLDKLIKAIALHIQNLIERIKIGNIVKHPNKDEIMKERAYEYNIAKEILSNMSLMY
                     GVEFPEDEAIFLATFLYLSYVGLNEESIAILVIMHGESTASSMVNVANTLLDCNHAVA
                     INMGLEDRVQDILVQAVEIANKIDKGKGILILTDMGSILTFAKVIRDATGKEVKAVDM
                     VSTPIVIEATRKALTPEMTLEKLYFNIIESIKKHYGDMEVTYAEKDNGGRYFDRLLID
                     NISKTLTFLNGEKAYFILKEVINRISEHYSLVIQDELLVKFIFHCSCMIERVIIKESL
                     VYKSYEERISRNKELYLVIKESFKLVEETFDIIISDMEYANILDIFESQYDTDKFKS"
     gene            4250..4654
                     /locus_tag="PC700_000909"
     CDS             4250..4654
                     /locus_tag="PC700_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2580232.1"
                     /translation="MIGIIVMTHGSFSEEIIKSCELIAGPAERTAAIKLNRNDNIEDL
                     NKNFVEKLNELDEGDGVLVLADLLGGSPSNVASLNLKKGGKFHALTGVNLPMLLEALI
                     NREGKSLEELAEACIEAGQTGINNINKVLSSM"
     gene            4678..5175
                     /locus_tag="PC700_000910"
     CDS             4678..5175
                     /locus_tag="PC700_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2580233.1"
                     /translation="MSLVKYCRCDDRLIHGQVIYKWVKHLGVKKIVVVDDETTNDVIA
                     KGLIKMAAPKNIDLSILTVSESRRYFYNNQADDNVFVLIKNLDTANRMIEEGINIKKL
                     IVGRIPTGIGKKKISQNVYINKKEFLLIDEFIKKNINVSIQMVPDEEEVELNQNINRY
                     KGEFI"
     gene            5178..5579
                     /locus_tag="PC700_000911"
     CDS             5178..5579
                     /locus_tag="PC700_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HDF2580234.1"
                     /translation="MNKYKVIILCHGTWGKVLVEETNKNFGLSNQYEVLSLSSEKDVS
                     VFMKEVEEVVDKEDVKIIISDLYGGSTSSVAIAVAIRRGIDAINGLSLQTILIVDEEL
                     SKEDKLHTLPERIVKKNNNLCVDLIKEFKKI"
     gene            5597..6085
                     /locus_tag="PC700_000912"
     CDS             5597..6085
                     /locus_tag="PC700_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2580235.1"
                     /translation="MAKITLVRVDHRLIHGQVITKWVKIAQAQKIIIVDDFLGQDEFM
                     ADIYKMAAPSGVEVVILTAEDAGQAFQNNTLGDKNIFILFKNVDMANKAYKAGLKYEK
                     IQLGGIPNEAGKKMVFTAVSLGNEDVEQLNELNENGVEIVLQVIPEESSMTYENALKK
                     FK"
     gene            6112..6867
                     /locus_tag="PC700_000913"
     CDS             6112..6867
                     /locus_tag="PC700_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2580236.1"
                     /translation="MESVLMLAIVTGLWYWFAAGLAGYTLFSTLKSPLFIGFSLGLLW
                     GDVTTGMIVGASIEMVYLGMVAAGGNIPSDKCLAALIAIPVALQTGVNAEVAVSIAVP
                     LGVIGVLVNNLRRTGNAVLVHKADKYAEEGNTKGIWRCATLYSLIFGFVLRFPIVFVC
                     NFFGADLVQSLLDVIPQWLMNGLTVMGGILPALGFATTIFTIGKNKFLPMFIIGFFMV
                     QYFEISITAAAIFGVCIALLITFMKEDKRVGEV"
     gene            6867..7730
                     /locus_tag="PC700_000914"
     CDS             6867..7730
                     /locus_tag="PC700_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF2580237.1"
                     /translation="MQEIKNKEVKNGQGPTEVNKTRVLTKKDVTKTYLRWWWTAELSN
                     SFERMQALAVCASFTPALEKLYKKKEDLVDALKRHLQFFNTQAIWGGLIHGTVLAMEE
                     EKATEGKIPGEVISGVKNGLMGPLAGIGDTLDFGTFQTIFLALGASFGAEGSVIGAFF
                     PIMFSILLFCEGYYLFHLGYSLGRDSIKKILSGGIVNKIIDGASILGMFMMGALSATT
                     VKLSTPLSFDIGGKAIVVQDTLNMIAPGLLPLGVVFFVYWGMKYKKWTITKLLVILVV
                     LALVGSFIGIF"
     gene            7777..8256
                     /locus_tag="PC700_000915"
     CDS             7777..8256
                     /locus_tag="PC700_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HDF2580238.1"
                     /translation="MSEKMYTEKEYLEGIRGATGERAVWFYLLMKEAEKLGANPDDIC
                     KEAIYGFGKMRGQKYNVADTPGKMAEMLYNSKGQKVFEMELVENTDENGVLKFHHCPL
                     DAAWKEYGLTKEERKEICRLACYGDYGRVDCAQGVKLDFAQKCAHDDEVCELVFTKK"
     gene            8321..9628
                     /locus_tag="PC700_000916"
     CDS             8321..9628
                     /locus_tag="PC700_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2580239.1"
                     /translation="MDISRSVDNSLNRTIEFLKELIKIDSQQGEPISQCPFGIGPKKS
                     LDKTLDYCASLGFSVKNIDNYIGYAEIGEGEELIGIPMHLDIVPPGEGWSVDPFSGAV
                     IDNIIYGRGVIDNKGAVSMLIHVLKNIEDMYPTINKRIRLIFGTNEETDMKCIKYYLD
                     KGEEIPSMGFTPDAMYPVVNGEKGRVHIRIEKEIKIDKSKPYIIVRGGTKENVVPSHC
                     TAKIINGIISELTTKGVAVHASNPEKGENAISKMVIKIVEDNMDFQHREDIELVSKYL
                     CSDYYGDALGINQYDEVFKNTTLNLGILKVNEEKIVCELDIRYGKNIVLNNIIDRFKK
                     VFCNGWKIEVIAHKDLHYVDESNLVLKKLLEAYEEVTDENGYTIAMGGGTYASWFKDM
                     VAFGPKFLAYKTGGHGVDERVPINHIRKNMEIYTLALIKLLEL"
     gene            9939..10079
                     /locus_tag="PC700_000917"
     CDS             9939..10079
                     /locus_tag="PC700_000917"
                     /inference="COORDINATES: protein motif:HMM:NF021468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HDF2580240.1"
                     /translation="MNGEGCILSNKITKECYKTGYMIREKSIDTYPDSGRGFMTWDEN
                     GE"
     gene            10591..11685
                     /locus_tag="PC700_000918"
     CDS             10591..11685
                     /locus_tag="PC700_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2580241.1"
                     /translation="MNELNIAKTLILKRKEKGITQDELANYIGVSKASVSKWETGQSY
                     PDITFLPQLATYFNITVDELICYEPQMMKEDINKLYNKLCKDFTVKPFDEVMDEIREI
                     IKKYYSCFPLIFRMGLLIVNHYDIVDEKKRELLISEALEIFIRIQETCNDIDICRQAK
                     SMEATCYILLNQPIQVIDLLQNSNFPMINESILLAQGQMMNGQMDEARETFQLGAYQN
                     LISLVQNLVGILQNADKLQMKEIERRILAISDIFELDTLSPAIMLSSYLTQTQINLIH
                     GDNEGAIKSLRKYVDLATRDIYPIIIHGDDFFNKLDRWISEIGTGITRDDTIVKAGIV
                     AAIKNNPMFSLLSENKEYKFLIEKLSLLEE"
     gene            11690..11890
                     /locus_tag="PC700_000919"
     CDS             11690..11890
                     /locus_tag="PC700_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc_N domain-containing protein"
                     /protein_id="HDF2580242.1"
                     /translation="MENLMEYLPLLIPVIILDLILIITALVHVLRHPNYKIGNKAIWI
                     IVVLFISLIGPILYFTIGRGEE"
     gene            11890..12807
                     /locus_tag="PC700_000920"
     CDS             11890..12807
                     /locus_tag="PC700_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2580243.1"
                     /translation="MNIVSINGLSKGFGNRKIIDNLNFTVPEGSVFGFVGKNGAGKTT
                     TMKMVLGLLEPDSGTIDVCGEKVTYGRTSSNRHVGYLPDVPEFYNYMRPLEYLSLCGE
                     ITGLSKKEIKIRSEELLSLVGLGNEKRRIGGFSRGMKQRLGIAQALLSRPKLLICDEP
                     TSALDPVGRKEILDIMLKIKDSTTVIFSTHILSDVERICDHVAILNKGSIALSGTLSE
                     IKSMHGKDRLLLEFASNNEIQKFKSSGGIKSLLKDSEETNMEIVLHGKDIKAIQKTVI
                     STLAEMNLCPVKMELIELSLENLFLEVVK"
     gene            12804..13562
                     /locus_tag="PC700_000921"
     CDS             12804..13562
                     /locus_tag="PC700_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2580244.1"
                     /translation="MREYIAFTKKEFKENIRNYKLLSLTILFLVFGIMSPLSAKFMPD
                     LIAHFAPTLKVTTAPTALDSWTQFSGNISGLGTSLTLIVFCNILSNEYSKGTLVIMLT
                     KGLSRSSVVLSKFSIAVIIMTIGFWLSFLCTYGYTMYFWPTANLNHIIFSAFNLWLIS
                     IMYISILILGCVLFRPAFASVLLVLAATAILSLISIPKQIAPYTPNFILSKNIDLVSG
                     KVTPPEFIIPIIVTIVISIVCLLLAVVLFNKKAV"
     gene            13945..15018
                     /locus_tag="PC700_000922"
     CDS             13945..15018
                     /locus_tag="PC700_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HDF2580245.1"
                     /translation="MEKSKVYFCDLYSNSQNKNVPNNVRRLFDEAGFKDLIEKNDQVA
                     IKLHFGEKGNTTYMSPVAVRQVVDKVKDCEGKPFLTDTNTLYTGSRTNSVDHLTTAIE
                     NGFAYAVVNAPVIIADGLYSRNYENVKIDKKHFESVKIGGEIYNSSAMIVMSHFKGHE
                     AAGFGGALKNLAMGCASAAGKQMQHSDVTPVVKEKKCVGCGKCVNSCPTKAISIVDKK
                     AVIDSDVCYGCGECPTVCPTRAVTIQWESDSDVFVEKMAEYAYGAVSNKKDKVGYITF
                     VMNVTPLCDCVPWSGRPIAHDIGILASTDPVAIEQACYDLICKEMGHDVFKHEHPHVN
                     GTRIIDYACEMGMGSKEYELIKL"
     gene            15088..15351
                     /locus_tag="PC700_000923"
     CDS             15088..15351
                     /locus_tag="PC700_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580246.1"
                     /translation="MKIYYEKENSKKANVNNIPSVFAFNFLRNFLTSKEYEETRKEYF
                     INNLTKSQVNQAMKDLKWLFREYEGLEVITMESLDGVKTRIVL"
     gene            15557..16534
                     /gene="bioB"
                     /locus_tag="PC700_000924"
     CDS             15557..16534
                     /gene="bioB"
                     /locus_tag="PC700_000924"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HDF2580247.1"
                     /translation="MEKYIIKLKNKVLREKEISYEEALNLISLDTNNKNDFDILLKSA
                     NEIREYFMGRKADLCTIMNAKSGKCSEDCKFCAQSSHYKTGVEEYSLLDYNEILNRAK
                     EMESKGVHRFSLVTSGKGMSGKEFNNILNIYEGLRENTNLKLCASLGIIDYEQAKMLK
                     SAGVTTYHHNVETCRDNFHNICTTHTYKDRIKTIKDAKKAGLDVCVGGIIGMNESEEQ
                     RLKMVFEIRELNVKSFPINILNPIKNTPMENYDVLEPMEILKTTAVFRFIIPNVYIRY
                     AGGRLSLKGYDKVGFNGGVNSAIVGDYLTTVGSGIENDKKMIIEQGFEL"
     gene            16762..17775
                     /locus_tag="PC700_000925"
     CDS             16762..17775
                     /locus_tag="PC700_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF2580248.1"
                     /translation="MSITIKEIGELAGVSKTTVSKVINNKDENISQATREKILKIMKE
                     KNYVPNKLAQSLVTKKTNTIGLLIPDIRNPFFTDVSRGVEDKANEEGYNIILCNTDED
                     AKKEYEGIRTLSERMIDGIIFAASSNTNWKEANYKDIKIPTVLIDKKISMNKETLKGI
                     VKINNFEGAYIATKHLLDIGNKKIIYLSGPLQNEIAVDRLEGYKKALIECNLSYNSAY
                     VFEGKYKIEWGQEFIKNLEKIDFDAIFCANDLIAIGVIRGLKERGLSIPNDVSVVGFD
                     DIQTSSLISPSLTTVKQPSYDIGYKASEILINCLRGDKKESFDELIFKPELVIRDSTK
                     ESE"
     gene            17817..18707
                     /gene="rbsK"
                     /locus_tag="PC700_000926"
     CDS             17817..18707
                     /gene="rbsK"
                     /locus_tag="PC700_000926"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDF2580249.1"
                     /translation="MGNIVVIGSVNMDMVCSVDKRPEKGETVLGNSFFTSPGGKGANQ
                     AISASKLGANVKMISCIGEDGLGEELIRNFRTNKVDYSLVSRNNHKSSGVAVITLCEN
                     DNSIVVVPGTNELVDIELIKKNEEEIKNADIVLLQLEIPLKTINYVVNFCFENRIKVL
                     LNPAPAVKLNEDIIEKVTYLTPNEHEYKIVFDTNEGIEEVLKKYPNKLVITEGKNGAR
                     FYDGEEIKHVSCISVDVQDTTGAGDTFNGALAVAITEGKKLYTAVEYAVVVSGLSVTK
                     LGAQSGMPYKEDVEKYLNNK"
     gene            18755..19717
                     /locus_tag="PC700_000927"
     CDS             18755..19717
                     /locus_tag="PC700_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDF2580250.1"
                     /translation="MRVRKILSLGIAGILAIGMLTGCSMEGPSKSDNKGGSDKKDLTI
                     GVSTITLQHQFFIDIDEGIKEKAKELGVKVIVNDPDQDVAKQTSAIEDFIQQNVDGMI
                     VLGTDNSAIVPAVEGAFEKMPVVTVDAVLNTENITSYVGTVSYDAGKKLGEYTKKYID
                     EKLGGKSEIAIVTDLKSQIQMQRIDGFKDALKGSANVKILNSQPGYDREESLNTVENL
                     IQSNPDVDIIYATAENSVLGAKAALESAKNKDVKIVGFDLTEEASSGITDGTILAMIQ
                     QQPKEMGRLAVEAVVKAIKGEKVEKNIPVPALLYDKENIKDFKN"
     gene            19771..21282
                     /locus_tag="PC700_000928"
     CDS             19771..21282
                     /locus_tag="PC700_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDF2580251.1"
                     /translation="MSNIILKLSNIAKEFPGVRALDNVNFELFHGEVHALLGENGAGK
                     STMIKILTGAHSKTSGKFIFEGKEIENISPDISKKIGINAIYQELTVFDELTVAQNIF
                     MGKEINGKVLTNDKKMNEEAKKIFDNMGIDINPNSLVKELSIAQKQMVEIARVLSSET
                     KVLIMDEPTSSISKKETEILFRLINDLKESGVSIIYISHRMEELFEICDRITIMRDGK
                     TISTLNTKDVSSEEELVNLMIDRKLDQFFPKRKVEIKEEIMRVENLTKNNVFNDISFN
                     IRKGEILGIGGLVGSKRSEIVEAIFGLRTYDSGKIYLNNEEVKFKTPSDAIENGLGLI
                     TEDRKGTGLFLQMSVKENTTMAGLKKISKFKSIIDRKKEKEILEKYIEALKIKTPHMN
                     QVIQSLSGGNQQKAIIARWLLLQPDILIMDEPTRGIDVNAKAEIYNLMGDLVESGVSI
                     IMISSEIPELISMSDRIMVMREGHISGFLEGEEMVENNVLKLAFGGKINEFNN"
     gene            21266..22264
                     /locus_tag="PC700_000929"
     CDS             21266..22264
                     /locus_tag="PC700_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HDF2580252.1"
                     /translation="MSSITKHKEEKSSPLLTKLTSVFRDQGSAAVGLVIIFIIMSVAS
                     SNFLTLDNLINVGRQISINAILAVGMTFVIITGGIDLSVGAVIALVGTFWATTVVNYN
                     APIWVGMILALAIGTILGVIKGAIISTQKLPPFIVTLAMLTIISGASFVFTGGRPISV
                     NTDAFKMLGRGYIGPIPIPVIIMIIVVIAGHFLLKRTNFGRHVHAVGGNEEAARLCGV
                     KVNKVIVKVYALAGLLTALAGIILSSRLASGSPTVGDGAELDAIAAVVLGGTNMMGGS
                     GSIVGTCIGVGIIGILGNGLNLLSVSSYNQMIIKGLVMLFAIWINNIKLKKSAKSK"
     gene            22448..23710
                     /locus_tag="PC700_000930"
     CDS             22448..23710
                     /locus_tag="PC700_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2580253.1"
                     /translation="MKKILLDTLNSKKQEYIDYLKELVSIKTEDVGHGILGGFEKEGQ
                     EYIEKLANYIGFSVDRQEMSEELIKKAKNIYKEGNLGHNYQDRYNLICKYSDDLPGKT
                     IVFNGHVDTMPPGDISKWKYNPYRATEDNGKLYGLGTADMKSGLIASILAVKLIKDSG
                     LNVPGNVKIMSVVDEEGGGNGTINAVMNGIGGDCCIICEPSEQNLIVAHMGFVFFEVE
                     VKGVSLHCGSKWEGVNAIEKAMLLLQDIKELEHNWLMIYKHPLLPSPTINLGVINGGT
                     AGSTVPDKCVFNLCVHFLPNIMSYEQVVNDVTNVIMTRANGDLWLKDNKPNINIYQSG
                     LGFQMDKDSDFVVNAHKILEETLGKKLEIKGSTAGNDARLMKNLAEIPTLILGPGSIE
                     QCHSIDEYVEIKEYLDSILMYASLILNL"
     gene            23730..24473
                     /locus_tag="PC700_000931"
     CDS             23730..24473
                     /locus_tag="PC700_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HDF2580254.1"
                     /translation="MKKVLIVGESWVKNITHIKGFDTFVTTHYEEAVKWLKEAIESGG
                     YETVHMPAHVAADSFPYKLEELNEYDCIILSDIGSNTFLLSNSTFIDCNSNPDRLELI
                     KEYVNNGGALIMVGGYMSFTGIDAKARFGETAIKDVLPITMIDKDDRVEKPAGIIPEV
                     IDSEHPVLKGIPTEWPKFLGYNKTVARDNCPVLATIGGDPFVAVGEFGKGKSAIFSSD
                     CAPHWGPKEFTDWKYYNKLWVNMLDWLTC"
     gene            24646..26031
                     /locus_tag="PC700_000932"
     CDS             24646..26031
                     /locus_tag="PC700_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2580255.1"
                     /translation="MDNLNKKFLMEYIDEIQGELRDISDKIWKNPELQYKEYYASNLQ
                     KECLKKHGFSIEGLEGMDTAFVASYGEGRPVIAVLGEYDALDGLSQKVSTTKEPIVDG
                     GAGHGCGHNLLGTGSIGAVIAVKELIKLKKLKGTIKYYGCPAEEDLSGKVLMIKKGFF
                     DGIDCAFSWHPFDINTPIRIPTLANYSVKFRYNGISAHAAQAPYNGRSALDAVELMNI
                     GCNYLREHIFDNCRVHYVTTNGGKMPNIVPDFAEVWYYIRGVKMEHVRDVFGRIVDIA
                     KGAALMTGTIMEYDIISGVYDYIPNTILTDILSQNMKLVGVQNYNEDDYYFADKLAET
                     VSIDKRSSVSSVLSGNKDITKMNLHDEVTDDTFTHNNCISLSLDIGDVSYIIPTAQCS
                     CSVWPIGISAHTWQSCASAGSDMGFKAMLLASKSISCSINDVMLDELVINKAKKELKD
                     TVGSFEYTPII"
     gene            complement(26368..27012)
                     /locus_tag="PC700_000933"
     CDS             complement(26368..27012)
                     /locus_tag="PC700_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2580256.1"
                     /translation="MNNLDKNNVNEVLDMLLRTRRSIRSFTKEVPSKGLIEEIIETGR
                     LAPYAILSVAKQTDFRHFFVISNNSLQMNKIKTILVDVIKTQISVNEKKAKNNITLQS
                     FITSLKMQVEKGVLGIGTAPYLIIVAERRGIPPIEQKSLSHVMQNMWLKSTALGLGFE
                     LVSVIGRLSDNKDFCDILGVPTGEYGFDACAIGFPTTSVTREGRAIPHPSIKWI"
     gene            27276..27890
                     /locus_tag="PC700_000934"
     CDS             27276..27890
                     /locus_tag="PC700_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HDF2580257.1"
                     /translation="MKKVSIDRRTIRTKKMIRSALAELIDEKGFNNISVTDLTQRADI
                     NRGTFYLHYVDKYDLLERIENEIIQEIEEETKHLDSSNMMEIDTSSEPLPFMVKLFEY
                     FRKNSVIIKSILGPKGDPTFERKIKKFIETNLFEKQKLINFNIDEAIISEEYFIQYIL
                     SADLGVIQYWLEKNMKESPEEMALILARMSLLGPLRAVGIRKDS"
     gene            28111..28251
                     /locus_tag="PC700_000935"
     CDS             28111..28251
                     /locus_tag="PC700_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580258.1"
                     /translation="MKNENISYLALGLCFGVVLGSLFKNLAIGLSFGLVIGVVLDSKK
                     NK"
     gene            complement(28481..28919)
                     /locus_tag="PC700_000936"
                     /pseudo
     CDS             complement(28481..28919)
                     /locus_tag="PC700_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895378.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT        11287 a         3047 c         5326 g         9262 t
ORIGIN      
        1 tataatttta aaaggagtga tataatatga gtaaatatag ttctttatgg gaatatgtta
       61 agaaaaataa tagtcaatcg tttaaattaa cttttgaaga aataaaagat attgctggaa
      121 tagagataga ccattcgttt ttgaaatata aaaaagaatt aaatgaatat ggatatcaag
      181 taggaaaaat ctctttaaaa gagaagactg ttattttcaa taaaattgat taagatataa
      241 ataattacat ataataaaat aatttgtatt gacgattatt acaaataaaa ataaagtaaa
      301 tcttatataa gatttacttt atttttagat gatatttttt tagctttata ctataaggta
      361 tataaaacta agcctattaa aaatgttata atttctatta ccatcataga ccatatcaat
      421 ccatcaagtt cataaaagta attaagcttt attaaatagc ttctttatta taatttcttt
      481 atttcatata atataaatag tattaaaatt tttatgggag ttaaagtaag catgtattat
      541 tacttagata tttgtataat caacatctag taatttacct ttttggtgaa aatgattaat
      601 aagtttttta ggataattca ttattaattc atctggaaaa ttaatttcct ttaagatatt
      661 ctcagaatac tcatagttac ctatgtcaaa acaaatgtga gcatctgaag ttagtatgat
      721 tttagcacca tattttttgc aaagtgtagc aatatactta cagttatccg agctaccatt
      781 tcttgacacg cctttaatag aacaattatt tatttctatt aagatattct tttctatagc
      841 taatttaatg attaattcat agtcgattgg gtagtttggg tttccaaggt gtcctaatat
      901 ttcaatatta tcatgcttat ttattgcatt tattaaagct ttagtattat tctctaatgt
      961 atttggagaa aaaacagctt cgtgaaatga caagataaga taatctaacc gaggaattac
     1021 aaaaggctct aggtctatgt ttcctttaat atctagtata tttgcttcac agcctcttaa
     1081 aattataatg ttatttatga ttcttgggat attttgaatg ttgtgaaagt accatctgtg
     1141 gggagaacca ggcatcttag gaccatgttc agatgtacct agtatcttaa ttccattttc
     1201 tttacaataa ttaatatttt ctagtaaagt gctatagcca tgaccactga ctatagagtg
     1261 ggtgtgtaaa tctgataaaa aattcatata aattcctcct ctataaaaga tgagggtaac
     1321 gtaatttata ttataaaacg aataggaaag aaaatcaata gttacataat tagacaaaaa
     1381 tattaaaata ttgtagaatt aagcttaata ataaatgttt aacagtatta ctgcttatag
     1441 gggaggttaa ctatgacaca aaagaagcta tcaaggaaag aaaaagtttt aatttgcttg
     1501 aaaaggttaa cagaagaaca cttgaacttt aataatggca ttagatttgg atttgatgca
     1561 gaatatatag ctactgaagt aggtataaat agaagtaatg ctagtaagga attaaatcaa
     1621 ttaataaaag aaagtattat tataaagata aagggtaagc ctgttcagta cttatataaa
     1681 gaaagtattg agagtatttt gggaaagaaa ctaaataaag ggatatttga taatattaac
     1741 tctataaacg aatatagtga aaatgattct atcaaagatg agtatatttt agagagtata
     1801 aaaaaagaga acaatatttt taatttagta ggatgtgatg gaagtttaag gtcccaaata
     1861 gaacaggcta aagcagcagt aatatatcca cctaaggggt tgagtacatt aataatagga
     1921 cctacaggag ttggtaaatc tatatttgca gaatatatgt ataagtatag tttaactata
     1981 aaagaagata gtgataatgc accatttatc atatttaact gtgctgatta ttctgataat
     2041 cagcaattat tattagcgca gctttttgga tatgtaaaag gagcatttac tggagctgat
     2101 aaagataagt atggtattgt acatgaagct aataatggag tattgttttt agatgaagtt
     2161 catagattat ctgcagaggg acaagaaatg ctatttctgc ttatggataa agggatatat
     2221 agaaggttag gagaggctaa taaggtacac gagtgtagag ttatgataat tgcagccact
     2281 acagaagacc cagaaacagc aatgttggaa acgtttttaa gaagaatacc tgtaactata
     2341 aagattccgt ctttagagga aagaggcttt caagagagaa tgaagcttat atgttatttt
     2401 tttaaagaag agagtgttag gattggtgtt aagttaaagg tttcaaaaga agttattaag
     2461 gcatttattt tatataagtg taagggaaac ataggccaat taaaaagcga tatacaatta
     2521 atttgtgcaa gagctttttt agattatatg acctataaaa gggaatgtgt atatgtaagg
     2581 ctctctcttc taccaaataa tattagagaa tcattatatg gaaataatag aaaagaggag
     2641 tttgtacaaa atttcagttt tattggaaaa gatgatatta tttttttacc agaagaaaag
     2701 gattttgaaa gtgaaaattt attaatagaa aataaaaaat atgatttaga tttttatgga
     2761 atgataaaag aaacttggaa taaacttcaa caagaaggta ttaaagagtc acaaattaga
     2821 agtgttatag atggagacat acagaagtat tcctataatt taatgaatac ttttatatat
     2881 aataatagta atcaaacagc ttataacaac attgttaatg attcaattgc aactactgta
     2941 aaatatattt tacaaaagca tgataaatgg agcaaaagag aaggattaga taaattaata
     3001 aaagctatag ccttacatat tcagaattta attgaaagaa taaagattgg aaatatagta
     3061 aagcatccta ataaagatga aattatgaag gaaagagcat atgaatataa catagcaaaa
     3121 gaaatattat ctaacatgtc attaatgtat ggagtagagt ttccagaaga tgaagcaatt
     3181 tttttagcaa catttcttta tttatcgtat gtgggattaa atgaggagag tatagcaata
     3241 ttagtcataa tgcatggtga atctacagca tctagcatgg tgaatgtagc caatacttta
     3301 ctggattgta accatgcagt ggcaattaat atgggattag aagatagagt tcaggatatt
     3361 ttagtacaag ctgtagaaat agccaataaa attgataaag gaaagggaat tttaatttta
     3421 actgatatgg ggtctatttt aacatttgct aaagttattc gtgatgctac aggaaaagaa
     3481 gtaaaagcag tagatatggt aagtacacct attgtaatag aagcaactag aaaggctcta
     3541 actccagaaa tgacattaga gaaattatat tttaatatta tagaatctat aaagaaacat
     3601 tatggagata tggaagttac atatgctgaa aaagataatg gtggtagata ttttgataga
     3661 ctactaattg ataatattag taaaactttg acttttttaa atggggaaaa ggcttatttt
     3721 atattaaaag aagtaattaa tagaattagt gaacactatt ctttagtaat tcaagatgaa
     3781 cttttagtaa agtttatatt tcattgttca tgtatgattg aaagagtaat aataaaagag
     3841 tctttagttt ataaatcata tgaagaaaga attagtagaa ataaagaatt atatttagtt
     3901 ataaaagaaa gttttaagct agtagaagag acattcgata taattatttc agatatggaa
     3961 tatgcaaata tattagatat atttgaatca caatatgata cagataaatt caaaagttga
     4021 tacaaaattt aaaaatagcc aaaaggctat ctttttttta ctttttttga tacagatttg
     4081 atacaggttg aaaaaatctt ttgatacata tcgaaataat aagtttttaa aaaaaattga
     4141 gtaaaaagtt ttgattaaat cctgtaaaat agccatttat tattgataca cataagttgg
     4201 cacggcaatt gcttatataa aaagtgacaa gaaaagtgag gtgaaattgg tgataggaat
     4261 aatagttatg actcatggga gttttagtga agaaattata aaaagctgtg aacttattgc
     4321 aggaccagca gaaagaacag cagcaattaa actaaatagg aatgataata ttgaagactt
     4381 aaataaaaat tttgtagaaa agttaaatga attagatgaa ggtgacggag ttttagtatt
     4441 agctgaccta ttaggaggtt cgccaagtaa tgttgcaagt ttaaacctta aaaaaggtgg
     4501 caagtttcat gcacttactg gagtaaatct tccaatgcta cttgaagctt taattaatag
     4561 agagggaaaa agtttagaag agttagcaga agcatgtatt gaagcaggac aaactggtat
     4621 aaacaatatt aataaagttc ttagctcaat gtaatagaaa aaggagtttg attaaaaatg
     4681 tctttagtaa aatattgcag atgtgatgat agattaatac atggtcaagt gatatataaa
     4741 tgggttaagc atctaggtgt aaaaaagata gtagtagtag atgatgaaac aaccaatgat
     4801 gttattgcta aaggtttgat aaaaatggca gcaccaaaga atattgattt atcaatatta
     4861 actgtaagtg aatcaaggag atatttttat aataatcaag ctgatgataa cgtttttgta
     4921 ttaattaaaa atttagatac tgcaaataga atgatagaag aagggattaa tatcaaaaaa
     4981 cttattgttg gaagaatacc tacaggaata ggaaaaaaga aaatatctca aaatgtatac
     5041 attaataaaa aagagtttct tttaatagat gaatttatta aaaagaatat taatgtatca
     5101 attcaaatgg tacctgatga agaagaagta gaattaaacc aaaatattaa tcgctataaa
     5161 ggagagttta tttagcaatg aataaatata aggtaattat actgtgccat ggaacttggg
     5221 gaaaagtctt agtagaagaa acaaataaaa actttggatt gtcaaatcaa tatgaagttt
     5281 tatcactaag ttctgaaaaa gatgtttctg tatttatgaa agaggttgaa gaagtagtag
     5341 ataaagaaga tgtgaaaatt ataatatcag atttatatgg aggatctaca tcaagtgttg
     5401 caatagctgt tgctataaga agaggaatag atgcaataaa tggacttagc ttacagacaa
     5461 ttttaatcgt agatgaagaa ttaagcaaag aagataaatt acatacatta ccagaaagaa
     5521 tcgtcaaaaa aaataataac ctttgtgtgg atttaattaa agaatttaaa aaaatataaa
     5581 ttatgaggtg aatataatgg caaaaataac attagtaaga gtagaccaca gattaattca
     5641 tgggcaagtt ataacaaaat gggtaaaaat cgctcaagct caaaaaataa taatagttga
     5701 tgacttctta ggtcaagatg aatttatggc tgacatatat aaaatggcag caccatcagg
     5761 agtagaagta gttattttaa cagcagaaga tgcgggacaa gcattccaaa acaacacttt
     5821 aggagataaa aatatattta ttctatttaa aaatgttgat atggcaaata aggcttataa
     5881 agctggactt aaatatgaaa agattcaatt aggaggtatt ccaaatgaag caggaaagaa
     5941 aatggtgttt acagctgttt cattaggaaa tgaggatgta gaacaactta atgaacttaa
     6001 tgaaaatggg gttgaaatag tcctacaagt tataccagag gaatcatcta tgacatatga
     6061 aaatgcgtta aagaaattta aataaaaaat ataaattagg aggaattata aatggaatct
     6121 gttttgatgt tggcaattgt aacaggatta tggtattggt ttgcagcagg tcttgcaggg
     6181 tatacccttt tctcaacact aaagtctcct ttatttatag gttttagcct tggattactt
     6241 tggggtgatg taacaactgg tatgattgtt ggtgcaagta tagaaatggt ttatctagga
     6301 atggtcgcag cagggggaaa tattccatca gataaatgtc tagctgctct tattgcaata
     6361 ccagtagcac ttcaaactgg tgttaatgca gaagttgcag tatctattgc ggtaccttta
     6421 ggtgttatag gagtattagt taataactta agaagaactg gtaatgctgt attggttcat
     6481 aaggcagata agtatgctga agaaggtaat acaaaaggca tctggagatg tgcaacactt
     6541 tattctttaa tatttgggtt tgttttaaga tttccaatag tttttgtatg taactttttt
     6601 ggagcagatt tagttcaatc attattagat gttataccac aatggttaat gaatggatta
     6661 acagtaatgg gcggtatatt acctgcatta ggttttgcaa caacaatatt tactataggt
     6721 aaaaataaat tcttaccaat gtttattata ggatttttta tggttcaata ttttgaaata
     6781 tcaattactg cagctgcaat atttggtgtt tgtatagctc ttttaattac ctttatgaaa
     6841 gaagacaaaa gggtaggtga agtataatgc aagaaataaa aaataaagaa gttaaaaatg
     6901 gacaaggtcc aactgaagtt aataagacaa gagtactcac aaagaaagat gtaacaaaaa
     6961 catacctaag atggtggtgg acagcagagt tatctaattc ttttgaaaga atgcaagcat
     7021 tagcagtttg tgcaagtttc actccggctt tagaaaagtt atataagaaa aaagaagatt
     7081 tagtagatgc attaaagaga cacttacaat tttttaatac tcaagctata tggggaggat
     7141 taattcatgg tacagtttta gctatggaag aagagaaagc tactgaagga aaaatcccag
     7201 gagaagttat ttcaggtgtt aagaatggtc ttatgggacc tttggctgga attggagata
     7261 ctttagattt tggtacattt caaacaatat tcttagcttt aggtgcttca tttggagcag
     7321 aaggaagtgt tataggtgca tttttcccaa taatgtttag tatattacta ttttgcgaag
     7381 gttattactt atttcattta ggatattctt taggtagaga ttcaattaag aaaattctat
     7441 caggtggtat tgtcaataag ataattgatg gtgcttcaat tctaggaatg tttatgatgg
     7501 gagcattatc tgcaactaca gttaagcttt caactccgct atcatttgat attggtggta
     7561 aagcaattgt tgttcaagat acattaaata tgattgctcc aggtttatta ccacttggtg
     7621 ttgtattctt cgtatattgg ggaatgaaat acaagaagtg gacaataact aagttattag
     7681 taattttagt tgtattagca ttggtaggat cattcattgg aatattctaa ttcacagtga
     7741 ttaaaatata gaatttaatt ttaggaggag aaagaaatgt cagaaaaaat gtatacagaa
     7801 aaggaatatt tagaaggaat tagaggagca acaggagaaa gagcagtatg gttctatctt
     7861 cttatgaaag aagctgaaaa attaggagct aatccagatg atatatgtaa agaagcaata
     7921 tatggatttg gtaaaatgag aggtcaaaaa tataacgtag cagatactcc aggaaaaatg
     7981 gcagaaatgt tatataacag taaaggacaa aaagtgtttg aaatggaatt agtagaaaat
     8041 acagatgaga atggcgtttt aaaattccat cactgtccat tagatgctgc ttggaaagaa
     8101 tatggattaa ctaaagaaga aagaaaagaa atttgtagat tagcttgtta tggggattat
     8161 ggaagagttg actgtgcaca aggtgttaaa ttagattttg ctcaaaaatg tgctcatgat
     8221 gatgaagttt gtgaattagt atttacaaaa aaataatttt taatgctaaa agcctgttct
     8281 ttaaggacag gctttaaaaa gatagaaagt aggttgattt atggatataa gcagaagtgt
     8341 agataatagt ttaaatagaa ccatagaatt tctaaaagaa ttgataaaaa ttgattcaca
     8401 acaaggtgaa cctatttcac aatgtccttt tggtatagga cctaaaaaat ctttagataa
     8461 aactttagat tactgtgcta gtttaggttt tagtgttaaa aatattgata attatattgg
     8521 atatgcagaa attggagagg gagaggaatt aatagggatt ccaatgcatt tagatatagt
     8581 tccaccagga gaggggtgga gtgtagaccc attttcggga gcagtaattg acaatattat
     8641 ttatggaaga ggtgtcatag ataataaggg tgctgtatct atgcttatac atgtattgaa
     8701 aaacatagag gatatgtatc ctacaattaa taaaagaata agattaattt ttggtacaaa
     8761 tgaggagaca gatatgaaat gtatcaaata ttacttagat aaaggtgaag aaattccaag
     8821 tatgggattt actccagatg ctatgtatcc agtggttaat ggagaaaaag gaagggttca
     8881 tataaggata gaaaaagaaa ttaaaattga caaaagtaaa ccttacataa tagtaagggg
     8941 aggaactaaa gaaaatgttg ttccatctca ttgtactgca aaaattatta atggaattat
     9001 ttcagagtta actactaaag gagtagctgt acatgcaagt aatcctgaaa agggagaaaa
     9061 tgctatatct aaaatggtaa taaagatagt tgaggataat atggattttc agcatagaga
     9121 agatatagaa ttagtttcaa aatacctttg tagtgattat tatggagatg cattggggat
     9181 taatcaatat gatgaggtat tcaaaaacac taccttaaat ttaggaatat taaaagtaaa
     9241 tgaggaaaaa atcgtatgtg agttagatat aaggtatggt aaaaatatag ttttaaataa
     9301 cataatagat agattcaaaa aggttttttg taatggttgg aaaatagagg ttattgctca
     9361 taaagactta cattatgtgg atgagagtaa tttagtactt aaaaaattat tagaagccta
     9421 tgaagaagta acagatgaaa atggatatac aattgctatg ggtggtggca catatgcaag
     9481 ttggtttaaa gatatggtgg cgtttggacc taagttctta gcgtataaaa ctggtggtca
     9541 tggagtagat gaaagagtac caataaatca tatacgaaag aatatggaga tatatacttt
     9601 ggctttaata aaactattag aattgtaaaa gaagaagaga ctttatgtgc aaatttacag
     9661 atatcttaaa aagagagata aaacgagttc aactttattt aaatgttcaa caccatcaaa
     9721 tggggctgtc tcaaaataga gataagcctc ttgcttatta ttgaaatgat gaaaatatat
     9781 tttacttcat acaaaaaaga gtgccttatg aagtaaaaag ttaattttga gacaccctca
     9841 tttgatgatg ttgaagagaa aataagtatg aaattattaa aaaaagatga ttttataaaa
     9901 gttaaggttg aaaataaaag ttaattgaat ggaattagat aaatggagaa ggctgtatac
     9961 tatctaataa aataacaaaa gagtgttata aaactggtta tatgattaga gaaaagtcta
    10021 ttgatactta tccagatagt ggacggggat ttatgacttg ggatgaaaat ggagaataat
    10081 aaaaattata gtttatacaa tttaataaga tatatgtaca agcagttaat ctaaatgatt
    10141 gactgctttt cttttataga tttttatata atagacaatt actaatacat gatagttgcc
    10201 tgttataatt agaggaatag atacgaataa taactttata aaagaattaa atggaaaatt
    10261 gaagagttat attcaggatt ttaaaaagaa tatgaaatga gaaatgaaaa taaggtgttt
    10321 atattaaaag aggttttaca caaaatactt aaaaacgtga taaataaaaa ctgtatttag
    10381 ataaattaac tactagtttt atatataata tggattttgt ttacaaagtt tacatatttc
    10441 acaaaatatt tttaaaatat atttaaaaat atattttata tacttgttcg tttgtttctc
    10501 tgtggttgat tttttcgtca aaagtcaact tcaaatcgct tgttagcttt acatgtttaa
    10561 ggatataatt atacataagg agggattgac atgaatgaat taaacatcgc aaaaacgctg
    10621 attttaaagc gtaaagaaaa aggaattaca caagatgaac ttgcaaatta tataggcgtt
    10681 tctaaagctt ctgtatctaa atgggaaact ggacaaagct atcctgacat aaccttttta
    10741 ccgcaactcg cgacatattt caacattacc gttgatgagt taatatgtta tgaacctcaa
    10801 atgatgaagg aagatattaa taaactgtat aataaactgt gcaaagactt tacggttaaa
    10861 ccgtttgatg aagtcatgga tgaaataaga gaaatcataa aaaaatatta ttcatgtttt
    10921 cctttgattt ttcggatggg tcttttaata gttaatcact atgatattgt agatgaaaaa
    10981 aagcgtgaac tattaattag tgaagcatta gaaatattta ttcgtataca ggaaacttgt
    11041 aatgacatag atatttgccg tcaagcaaaa agtatggagg caacttgtta tatattgtta
    11101 aatcaaccca tacaagtgat tgatttattg caaaacagca attttcccat gataaatgaa
    11161 tctatacttc ttgcacaggg gcaaatgatg aatggtcaaa tggatgaagc aagagaaaca
    11221 tttcaacttg gtgcatatca gaaccttata tcattagtcc aaaatctagt gggtattttg
    11281 caaaatgcag ataaattaca aatgaaggaa atagaaagac gtattctagc aatttcagat
    11341 atatttgagc ttgatacatt atctcctgct attatgcttt cttcatattt aacacagaca
    11401 caaattaatt taatacatgg ggataatgaa ggagctatta aatcattacg aaaatatgta
    11461 gatttagcaa cacgtgatat atacccaatt ataatacatg gggatgactt ttttaataaa
    11521 cttgacagat ggatttctga aattggaaca ggtattacac gtgatgatac aatcgtaaaa
    11581 gcaggtattg ttgctgctat taaaaataat ccaatgtttt ctttattaag tgaaaacaaa
    11641 gaatacaaat ttcttataga aaaattatct ttattggagg aataagatta tggaaaattt
    11701 gatggaatat ttaccacttt tgattcctgt tattatttta gatttaatac ttataattac
    11761 tgcacttgtt catgtattga gacatccaaa ttataaaatt ggaaataagg caatatggat
    11821 tattgtagta ctgtttatta gtctaatagg accaatcttg tattttacaa taggaagagg
    11881 tgaagaatga tgaatattgt ctcaataaat ggattatcaa aaggttttgg taatcgaaag
    11941 ataatagata atctaaattt tactgtacca gaagggtcag tctttgggtt tgtaggtaag
    12001 aatggtgcag gtaagactac aacaatgaaa atggtacttg ggttacttga accagatagt
    12061 ggaactatag atgtttgtgg tgagaaagtg acatatggaa gaacaagttc taatcgtcat
    12121 gtaggttatc tgcctgacgt tccagagttt tacaactata tgagaccact tgaatatctt
    12181 tctctttgtg gtgaaattac ggggctttca aaaaaagaaa taaagataag gagtgaagag
    12241 ttgttatcac ttgtggggct tggcaatgaa aagagacgaa taggtgggtt ttctcgtgga
    12301 atgaagcaac gacttggtat agctcaagct ttgctttcac gtcccaaatt acttatttgt
    12361 gatgaaccaa caagtgcact tgaccctgtt ggtcgaaaag aaatattaga tattatgctt
    12421 aaaatcaaag actcaacaac tgtaatattt tcaacacata tactatcaga tgttgaaaga
    12481 atctgtgacc atgttgctat actaaacaaa ggaagcattg ctcttagcgg tacactttca
    12541 gaaataaaaa gtatgcacgg aaaggataga cttttactgg agtttgcaag taataatgaa
    12601 attcaaaagt ttaagtctag tggtggcatt aaatccttac taaaagattc agaagaaact
    12661 aatatggaaa ttgttttgca tggtaaagat ataaaagcaa tacaaaaaac agttatatct
    12721 acactcgcag aaatgaatct ttgtccagta aagatggaac taatagaatt gtcacttgaa
    12781 aatttgtttt tggaggtagt aaagtgagag aatacatagc atttacaaaa aaagagttta
    12841 aggagaacat aagaaattat aaattactta gtttgactat tttattttta gtatttggaa
    12901 taatgagtcc tctttctgca aaatttatgc cagacttgat tgctcatttt gctccaactt
    12961 taaaagttac tactgcacca actgctttag attcttggac acagttttct ggaaatattt
    13021 ctggtcttgg aacgagttta actctaattg ttttttgcaa tattttatca aacgaatatt
    13081 ctaaaggaac actcgttatt atgttgacta aagggttatc acgttcatct gtagtattgt
    13141 ctaaattttc tattgcagta attattatga caattggttt ttggttgagc tttttatgta
    13201 cttatggata tacaatgtat ttttggccaa ctgcaaatct taatcatatt atattttcag
    13261 catttaactt atggttaatt agtattatgt atatttccat attaattctt gggtgtgtac
    13321 tatttagacc agcatttgca agtgtgttac ttgtattagc agcaactgct attttgagtt
    13381 tgatttctat tcctaaacag attgcacctt atacgcctaa tttcatatta tcaaaaaata
    13441 tagatttagt ttcaggtaaa gtgacacctc cagaatttat tattccaatc attgtaacaa
    13501 ttgttatttc gatagtatgt ctgttattag ctgttgtttt gtttaataaa aaagcagttt
    13561 aaggatattt ttattataaa tacaaatagt gctgtattgt gggaaaatgt gtttagtatt
    13621 gtagaagaat atatttaagt aaaaaagagg ctgtctcaag atagagaaat aagcctcttt
    13681 tttattttag tcctcctcac tatactttat aataattaag ttattacctt aaggaactat
    13741 ctaaaaaaag agatatagat tataatctaa tgcttatcag ttatttgagg tgtttagtat
    13801 atagagtcta aagtagtgaa ggactaaaat aatattgttt tcattaaata aagataagaa
    13861 tctttaattg tggtaaaata tactaaaaaa tcattttaaa agtgataaaa ttattttaag
    13921 taaatatttt aaaggggtgt agatatggaa aagtcaaaag tatatttttg tgatttatac
    13981 tcaaattcgc aaaataaaaa cgtaccaaac aatgtaagaa gattatttga tgaagcagga
    14041 ttcaaagatt taatagaaaa gaatgaccaa gtcgcaatta aacttcactt tggagaaaag
    14101 ggaaatacta catatatgag tcctgtggca gttagacaag tggttgataa ggttaaagac
    14161 tgcgaaggaa agccattttt aacagataca aatacactgt atacagggag tagaacaaat
    14221 tctgtagacc atcttacaac tgctatagaa aatggatttg cttatgcagt tgtaaatgca
    14281 ccagtaataa ttgcagatgg attatatagt agaaattatg agaatgtaaa aatagacaaa
    14341 aaacattttg aaagtgtaaa aataggtgga gaaatttata attcttctgc aatgatagta
    14401 atgagtcatt ttaaaggtca tgaagcagca ggttttggag gagcattaaa aaacttagct
    14461 atgggatgtg caagtgcagc aggaaaacaa atgcaacatt ctgatgttac tccagtagta
    14521 aaggaaaaga agtgtgtagg ttgtggaaag tgtgttaatt cttgtcctac aaaagctata
    14581 agtatagtgg ataaaaaagc agttatagat tctgatgtat gttatggatg tggagaatgt
    14641 ccaactgtct gtccaactag agcagtaact atacaatggg aaagtgactc agatgtgttt
    14701 gtcgaaaaga tggcagaata cgcttatgga gcagtatcaa ataaaaaaga caaggtaggg
    14761 tatataactt ttgttatgaa tgtaactcca ctttgtgatt gtgtaccttg gtcaggtaga
    14821 ccaattgccc atgatatagg aatattagca tcaacagacc ctgtagctat tgaacaagca
    14881 tgctatgatt tgatttgtaa ggaaatgggt catgatgttt tcaaacatga acatcctcat
    14941 gtaaatggaa ctagaataat agactatgca tgtgaaatgg gtatgggaag taaagaatat
    15001 gaattaataa aattataaga atagtagcac ttaaatgcat ataattattg taagagaaat
    15061 ctaaaagtat caaaggagta tgcatttatg aaaatttact atgagaagga aaacagcaag
    15121 aaggctaatg taaacaatat tccaagtgta tttgcgttta attttctaag aaactttttg
    15181 actagcaaag agtatgagga aactagaaaa gaatatttta taaataatct aacaaaaagt
    15241 caggttaatc aagccatgaa ggatttaaaa tggctattta gagaatatga aggcttagag
    15301 gttattacaa tggaaagctt ggatggagtt aagacaagga tagttttata aaccagtaag
    15361 aattatattc taataaaaaa tactgcttta ataataatat gtataatcta tatactaata
    15421 ttattttgaa gcagtatttt tttgtgtata gtaaacttgt gtctaaaata aagttgacca
    15481 ataaatgtta tatatgcaat actatatata ataagtaaac tacataaaaa tattaagttg
    15541 acaaatagga ggatatatgg aaaaatacat aataaaatta aaaaataaag tattgagaga
    15601 aaaagaaata agttatgaag aagctcttaa tttaataagc cttgatacaa ataataaaaa
    15661 cgattttgat attctattaa aatctgcaaa tgaaataaga gaatatttta tgggaagaaa
    15721 agcagattta tgtacgataa tgaatgctaa atctggcaaa tgtagtgaag actgtaaatt
    15781 ttgtgctcaa tcatctcatt ataaaactgg tgtagaagaa tattcattat tagattacaa
    15841 tgaaatctta aatagagcta aagagatgga gagtaaagga gtgcatagat tctcattggt
    15901 tacaagtggt aaaggtatgt caggaaagga gtttaataat attttaaata tctatgaagg
    15961 attgagggaa aatactaatt taaaattatg tgcatcttta ggtattatag attatgagca
    16021 agctaaaatg ttgaagtcag caggtgtgac tacatatcac cacaatgttg agacgtgtag
    16081 agataatttt cataatatat gtacaacaca cacatataaa gataggataa aaactataaa
    16141 agatgctaaa aaagctgggc tagatgtatg tgtaggcggt ataattggaa tgaatgagag
    16201 tgaagaacag agactaaaaa tggtttttga aatacgagaa ttaaatgtaa aatcatttcc
    16261 aataaacatt ttaaatccta ttaagaatac gccaatggaa aattatgatg tattagaacc
    16321 aatggaaata ctaaaaacaa cagcagtatt taggtttata attcctaatg tgtacataag
    16381 gtatgctggt ggaaggctat cattaaaggg atatgacaaa gttggattta atgggggagt
    16441 aaattctgca attgttggtg attatttgac aactgttgga agtgggattg aaaatgataa
    16501 aaaaatgatt atagagcaag gatttgaact ttaataaata ctacttaaat taattaatat
    16561 acttttagat aattataaaa gtatattaat tttttttgca gattaattta aatattaaaa
    16621 ttgaattata agttgcaata ttttgaaaaa agggaaaaat atcaaaattt atataaaatg
    16681 gttgcaaatg ggaaaaggat gtgatagtat ctaggtaacc ggttaagtaa aacggtttcc
    16741 taaattttgg gggtgaactt cttgagtata actataaaag aaattggcga attggctggt
    16801 gtttctaaaa caactgtgtc aaaagtcatt aataataaag atgaaaatat aagtcaggca
    16861 acaagagaaa aaattttgaa aataatgaag gaaaaaaatt atgtaccaaa taaattagca
    16921 caaagtttag taactaaaaa aactaatact atagggcttc ttataccaga tatacgaaat
    16981 ccattcttta cagatgtatc aagaggtgta gaagacaaag ctaatgaaga aggctacaat
    17041 ataattttat gtaatacaga tgaagatgct aaaaaagaat atgaaggaat aagaacacta
    17101 tcagagagga tgatagatgg aattatattt gcagcatctt caaatacaaa ctggaaagaa
    17161 gcaaattata aagatataaa aataccaact gtcttgattg ataagaagat aagtatgaac
    17221 aaagaaacct taaaaggaat tgttaaaata aataactttg aaggggccta tatagctaca
    17281 aaacatttat tagatattgg aaataagaaa ataatatacc taagtggtcc acttcaaaat
    17341 gaaatagcag tagatagatt agaaggatat aaaaaggctc taatcgagtg taatttaagc
    17401 tacaattctg cttatgtatt tgaaggaaaa tataagattg aatggggaca agaatttata
    17461 aaaaatttag aaaagataga ttttgatgca atattttgtg caaatgattt gattgcaata
    17521 ggtgtcataa gaggtcttaa agaaagaggg ttaagtatac caaatgatgt aagtgtcgtg
    17581 ggatttgatg atattcaaac atcaagtcta ataagccctt ctcttaccac agtaaaacag
    17641 ccgtcttatg atataggata taaagccagt gaaatattga taaattgttt aagaggagat
    17701 aaaaaagaat catttgatga attgattttc aaaccagaat tagtcataag agattcaaca
    17761 aaagaaagtg aataacagca cgagagacat aaatagatat gtaaaggagt aatgtaatgg
    17821 gaaacatagt tgtcatagga agtgtaaaca tggacatggt atgttctgta gataaaagac
    17881 cagaaaaagg agaaacagta ttaggtaata gtttttttac atcacctgga gggaaagggg
    17941 ctaatcaagc tatctcagct tctaaactag gagcaaatgt aaaaatgata tcatgcatag
    18001 gggaagatgg cttaggagag gagttaataa gaaattttag aacgaataaa gttgattata
    18061 gtttagtatc cagaaataat cacaaaagtt ctggtgttgc tgttataaca ttatgtgaaa
    18121 atgataatag tattgttgtt gtaccaggga cgaatgagct agtagatata gaattaatta
    18181 aaaagaatga agaagagata aagaatgcag atatagtatt gctacaatta gagattccat
    18241 taaaaacaat aaattatgta gtgaatttct gttttgaaaa taggattaag gttttattaa
    18301 atccagcacc agcagtaaaa ctaaatgaag atataataga aaaagtaact tacttaacac
    18361 caaatgaaca tgaatataag atagtttttg acacaaatga agggatagaa gaagtattaa
    18421 aaaaatatcc aaataaactt gtaataacag aaggaaaaaa tggagctaga ttttatgatg
    18481 gtgaggaaat caagcatgta tcttgtataa gtgttgatgt tcaagatacc acaggagcag
    18541 gagatacatt taatggagca ttggcagtgg ctataacaga agggaaaaaa ttatatacag
    18601 cagtagaata tgcagtagta gtatcaggtc tttctgtaac taaactaggt gcacaatctg
    18661 gtatgcctta taaagaagat gttgaaaaat atttaaataa taaataaaaa tctaatttgt
    18721 tcaaatttaa aactattaaa aatggaggtt aattatgaga gtgagaaaaa ttttatcttt
    18781 aggaattgct ggaatactag caataggaat gttaacaggg tgttcaatgg aaggaccaag
    18841 taaatcagat aataaaggtg gttcagacaa gaaagattta actattggag tatctacaat
    18901 aactcttcaa catcaatttt ttatagatat tgatgaggga ataaaagaaa aagctaagga
    18961 attaggtgtg aaagttatag taaatgaccc agaccaagat gtagctaagc aaacatcagc
    19021 aatagaagat tttatacaac aaaatgtaga tggaatgata gtacttggaa ctgataattc
    19081 ggctatagta ccagcagtag aaggagcatt tgaaaaaatg ccagtagtta cagtagatgc
    19141 tgtattaaat actgaaaata ttacaagtta tgtaggaaca gttagttatg atgctggtaa
    19201 aaaattaggg gaatacacta aaaaatacat agatgaaaag ttaggtggaa agtcagaaat
    19261 agcaatagtg acagatttaa aatctcaaat acaaatgcag agaatagatg gatttaaaga
    19321 tgcgttaaaa ggttcagcaa atgttaagat attaaattca caaccaggat atgacagaga
    19381 agaatctctt aatacagtag aaaacttaat tcaatctaat ccagatgtag atataatata
    19441 tgcaacagct gaaaatagtg tactaggagc taaagctgca cttgaatctg caaaaaataa
    19501 ggatgttaag attgtaggat ttgacttaac tgaagaagca tcaagtggaa taacagatgg
    19561 aactattctt gctatgattc aacaacaacc taaagagatg ggacgtttag ctgtagaagc
    19621 agtagtaaaa gctataaaag gggaaaaggt agaaaagaat ataccagtac cagctttact
    19681 ttatgataag gaaaatataa aagactttaa gaattagtaa aataaaatga gattatttga
    19741 aagaaatcct atttaaaaag gagtgtcatt atgagtaata taattctgaa gttaagtaat
    19801 attgctaaag aatttcctgg tgtcagagca ttagataatg ttaattttga gttatttcat
    19861 ggcgaagttc atgcactact gggtgaaaat ggtgctggta aatcaacaat gataaagatt
    19921 ttaactggtg cacattcgaa aacttctgga aaatttattt ttgaaggcaa agaaatagag
    19981 aatatatccc cagatatctc taaaaagata ggtataaatg ccatatatca agaactaaca
    20041 gtttttgacg aattaacagt agcacaaaat atttttatgg gaaaagaaat aaatggcaaa
    20101 gtcttaacca atgacaaaaa aatgaatgaa gaggctaaaa agattttcga taatatggga
    20161 atagatataa atccaaatag tttagtcaaa gagcttagta tagctcaaaa acaaatggta
    20221 gaaatagcaa gagttttatc atcagaaact aaagtattaa taatggatga acctacttct
    20281 tcaataagta aaaaagagac tgaaatactt tttagactta taaatgattt aaaagagagt
    20341 ggagttagta taatatatat atcccatagg atggaagaac tctttgagat ttgtgacaga
    20401 ataactataa tgcgagatgg gaaaacgatt tcgactttga ataccaaaga tgtatcgtct
    20461 gaggaagaat tagttaattt aatgatagat agaaagctag accaattttt cccaaaaaga
    20521 aaagtagaaa taaaagaaga aattatgcga gtggaaaatc taactaagaa caatgttttt
    20581 aatgatatta gctttaatat aagaaaaggt gaaatattgg gaataggtgg actagttgga
    20641 agtaagagaa gtgaaatagt agaagctata tttggactta gaacatatga ttctgggaaa
    20701 atatatttaa acaatgaaga agtaaagttt aaaactccat cagatgcaat agaaaatgga
    20761 ctgggattaa taacagaaga tagaaaaggt actggattgt ttttacaaat gagtgtcaaa
    20821 gaaaacacaa ctatggctgg gttaaaaaag atatctaagt ttaaatcaat tatagataga
    20881 aaaaaggaaa aagagatttt agaaaaatac atagaagctt taaagataaa aacaccacat
    20941 atgaatcaag ttatacaaag tttaagtggt ggaaaccaac aaaaagctat aatagcaaga
    21001 tggcttttat tacaaccaga tatattgatt atggatgaac caaccagagg tatagatgta
    21061 aatgctaagg cagagatata caatctaatg ggagatttag tggagagtgg tgtaagtata
    21121 ataatgatat cttctgaaat accagaatta atatcaatga gtgacagaat aatggttatg
    21181 agagaaggtc atataagtgg atttttagaa ggtgaagaaa tggttgaaaa taatgtatta
    21241 aaattggctt tcggaggtaa aattaatgag ttcaataact aagcataaag aagaaaaatc
    21301 ttctccatta ttgacaaaat taacaagtgt atttagagac caagggagtg cagctgtagg
    21361 acttgtaata atttttataa taatgagtgt agcttcatct aactttttaa ctttagataa
    21421 tttaattaat gttggaagac aaatatcaat aaatgcaata cttgcagtag gtatgacatt
    21481 tgtaataatt acaggaggaa tagatttaag tgtaggagca gtaattgctt tagtaggtac
    21541 attttgggct acaacagtag tgaattataa tgcacctata tgggtaggta tgatacttgc
    21601 attagcaata gggacaatat taggagtcat aaaaggtgca attatttcaa cacaaaaact
    21661 acctccattc attgtaactt tagccatgtt aacgataata agtggggcaa gctttgtatt
    21721 tacaggagga agacctatat cagtaaatac agatgcattt aagatgctcg gtagaggata
    21781 tattggacca ataccaatac cagttataat tatgattata gttgtaatag caggacattt
    21841 tctattaaaa agaactaatt ttggacgtca tgtacatgca gtaggtggaa atgaagaggc
    21901 cgctagatta tgtggagtaa aagtaaataa agttatagta aaagtatacg cactagctgg
    21961 actactaaca gcattagctg ggataatact atcatcacgt ttggcatcag gttcaccaac
    22021 tgttggagat ggagcagaat tggatgctat agcagccgtt gtgcttggtg gtactaatat
    22081 gatgggtggt tctggttcta tagtaggtac atgtataggt gtcggaataa taggaatatt
    22141 aggaaatgga ttaaacttat taagtgtatc ttcatataat caaatgatta taaaaggttt
    22201 ggtaatgtta tttgctatat ggataaataa tataaaatta aaaaaatcag ctaaatctaa
    22261 atagtaatta aattttagct tataaactat aaaaattatt aagtttttgt atatattttt
    22321 atactttaat ttgcatacat ctttatacat tgaatgggaa tatgctaagt gaaaaagttt
    22381 actatggctt aaatagatga gattagactt gaaattttaa atgtaaaaat aaatcttagg
    22441 aggaaatatg aaaaaaattc tcttagatac attaaatagt aaaaaacaag aatacataga
    22501 ttatttaaaa gaacttgtaa gtataaagac tgaagatgtt ggtcatggaa tactaggtgg
    22561 atttgaaaaa gaaggacagg aatatattga aaaacttgca aattacatag gttttagtgt
    22621 agatagacaa gaaatgagcg aagaattaat taaaaaagca aaaaatatat ataaagaagg
    22681 aaatctaggt cacaattatc aagatagata taatttaatt tgtaagtata gtgatgattt
    22741 acctggtaag acaattgtat ttaatggtca tgtagacact atgccaccag gtgatataag
    22801 taagtggaaa tataatccat atcgtgctac agaagataat ggaaaacttt atggtcttgg
    22861 aactgctgat atgaaatctg gacttatagc atcaatatta gctgtaaagc ttataaaaga
    22921 tagtggtcta aatgtacctg gtaatgtaaa aataatgtca gtagtagatg aagaaggtgg
    22981 cggaaatggt accataaatg cagttatgaa tggtattggt ggagattgtt gcataatttg
    23041 tgaaccatca gaacaaaact taatagttgc acatatggga tttgtattct ttgaagttga
    23101 ggttaaaggt gtgtctcttc actgtggtag taagtgggag ggtgtaaatg caattgagaa
    23161 ggctatgttg ttgcttcaag atataaaaga gttggaacat aactggctta tgatatataa
    23221 gcatccacta ttaccttcac ctacaataaa tttaggtgta ataaatggtg gaacagcagg
    23281 ttctactgtg ccagataagt gtgtatttaa tttatgtgta cacttcttgc caaatataat
    23341 gagttatgaa caagttgtaa atgatgtaac aaatgtcata atgacaaggg ctaatggaga
    23401 tttatggctt aaagataata aaccaaatat aaacatatat caatcagggt taggttttca
    23461 gatggacaag gattctgatt ttgtagtgaa tgcacataaa atactagaag aaactttagg
    23521 aaaaaaatta gagataaaag gttcaacagc tggcaatgat gcgagattaa tgaaaaattt
    23581 agctgaaata ccaacactta tattagggcc aggttcaata gaacaatgtc attcaattga
    23641 tgaatatgtt gaaattaagg agtatttaga tagtatactt atgtatgcaa gtctaatatt
    23701 aaacttataa aagtaggagg aatatataaa tgaaaaaagt attaatagta ggggaatctt
    23761 gggtaaaaaa tataactcat ataaaaggat ttgatacgtt tgttacaact cattatgaag
    23821 aagctgtaaa gtggttgaaa gaagcaatag aatctggagg atatgagaca gttcatatgc
    23881 ctgcacatgt agcagcagat agttttccgt ataaattaga agagttaaat gagtatgact
    23941 gtataatatt atctgatata ggttctaata cgttcttatt atctaatagt acttttatag
    24001 attgcaatag taatccagat agacttgaac taatcaaaga gtatgtaaat aatggtgggg
    24061 cgttaatcat ggtaggtgga tatatgtcat ttacaggaat agatgcaaaa gctagatttg
    24121 gagaaactgc tatcaaagat gtattaccaa taacaatgat agataaagat gatagagttg
    24181 aaaaaccagc aggaataatt ccagaagtta ttgactctga acatccagta ttaaaaggta
    24241 taccaacaga atggccaaag tttttaggat ataacaaaac agtagctaga gataattgtc
    24301 ctgttttagc aactattgga ggagacccat ttgtagctgt tggcgagttt ggaaaaggaa
    24361 aatctgctat ctttagttct gactgtgcac ctcactgggg tccaaaagaa tttactgatt
    24421 ggaaatacta taataaatta tgggttaata tgcttgattg gttaacatgc taaaaatttt
    24481 ataaaacttg aaaacctctt acttgataaa atgaaactta caagtcaaga ggttttattg
    24541 tataaaatgg ttgtatggta gaaaatattt ttaaatcagt taaaattagt cattttggct
    24601 gataatcgat aagttattag aaacagggag gtttatataa ttaatatgga taatttaaat
    24661 aaaaagtttt tgatggaata tatagatgag atacagggag aattaagaga tatatcagat
    24721 aagatatgga aaaatccaga acttcagtat aaagaatact atgcttcaaa tttacaaaaa
    24781 gaatgtttaa aaaaacatgg ttttagtata gagggattag aaggtatgga tacagcattt
    24841 gtggctagtt atggagaagg tagaccagtt atagcagttt tgggagaata tgatgcttta
    24901 gatggtttgt ctcaaaaagt atccactact aaagaaccta ttgtagatgg tggtgcaggt
    24961 catggatgtg gacataactt acttggtaca ggttcaatag gagctgtaat tgcagtaaag
    25021 gaattaatca aactaaaaaa gctgaaaggg actataaaat actatggatg tccagcagaa
    25081 gaagatttat ctggaaaagt attgatgata aaaaaaggtt tttttgatgg aattgattgt
    25141 gcttttagtt ggcatccatt tgatataaat actccgataa gaatcccaac acttgctaat
    25201 tactctgtga aatttagata taatgggatt agtgctcatg cagcacaagc tccttataac
    25261 ggaagaagtg cacttgatgc agtagaactt atgaatatag gttgtaatta tttgagagaa
    25321 catatatttg ataattgtag agtacactat gtaactacta atggtggaaa aatgccaaat
    25381 atagttcctg attttgcaga agtgtggtat tacataagag gagttaaaat ggagcatgta
    25441 agagatgtat ttggtaggat tgttgatata gcaaaaggag cagcattaat gactggtact
    25501 attatggaat atgacattat aagtggagta tatgactata ttccaaatac aatattaaca
    25561 gatattctat cacaaaatat gaaattagta ggtgtgcaaa actataatga agatgactac
    25621 tattttgcag ataaattagc tgagacagtg tctatagata aaagaagtag tgtttctagt
    25681 gtattaagtg gaaataagga tattactaag atgaatttac atgatgaggt tacagatgat
    25741 acatttactc ataataactg tatatcatta tcattggata ttggagatgt tagctacata
    25801 attcctacag cacaatgttc ttgttcagtt tggcctattg gtatttctgc ccatacatgg
    25861 caatcttgtg ccagtgcagg ttctgatatg ggatttaagg caatgttatt ggcatctaaa
    25921 tctatatcat gttcaataaa tgatgtaatg cttgatgaat tggttataaa taaagcaaaa
    25981 aaagaattga aggatactgt aggtagtttt gaatatactc caattatttg aagagtaatt
    26041 taatacaagt aagattgaat tgtatgaatt aggatgaata tataaattaa tattattttt
    26101 aacttaactt tctaattatt ttcaatctaa gatacaataa ttagaatata tttttaaagt
    26161 ttcctattta aggaaaataa tttgatttta tgtttgataa aaggacttat aatttgtaag
    26221 cccttttatt atatttattt tttgtttata attaatttta ttttttaggt ttagttaggt
    26281 tatagattac cctgaaaaat aacatttaac ttattattaa aaacatgtaa gaatagttta
    26341 taaatatttt attttcgatg ttgtttatta tatccatttt atcgatggat gaggaatggc
    26401 tctgccttcc ctagttacag atgtagtagg gaaaccaatt gcacaggcat caaatccata
    26461 ttcacctgtt ggtactccta aaatatcaca aaaatcttta ttatcgctca atcttcctat
    26521 tacactaaca agttcaaaac ctaatcctaa agcagttgat ttaagccaca tattctgcat
    26581 cacatgagat aatgactttt gttctatagg tggtattcca cgacgttctg ccacaataat
    26641 aagataaggt gctgttccta taccaagaac ccctttttca acttgcattt ttaaggaagt
    26701 aatgaaggat tgaagggtaa tgttgttctt tgccttcttc tcattgactg atatttgagt
    26761 tttaataaca tcaacaagaa tggtttttat tttattcatt tgtaaactgt tatttgaaat
    26821 aacaaagaaa tgtctgaaat ctgtttgttt tgctactgaa agtattgcat aaggtgcaag
    26881 tcttccagtt tcaattattt cttcaatcag cccttttgaa ggtacttctt ttgtaaaact
    26941 tcttatggat cttctagttc ttaatagcat atccaaaact tcattaacat tgtttttatc
    27001 taaattgttc atttttatcc tccactaata gtattcttaa gatgttctat aatgcatcca
    27061 tataaaggcg aacttttcgg tcatattaaa catttccttc aaattaagtc gctatttcta
    27121 tgaacacaat gttgattaat aatctatatg tatattataa tatttatatt gcattatgca
    27181 ataaacaata atatttaaac tgttaagtaa taaacattta ctataaaatt gttaattaaa
    27241 attaaaaaaa tttatatgga atgaggaaat aattcatgaa aaaagtatct attgatagaa
    27301 gaactatacg tactaaaaaa atgattaggt ctgcattagc agagttaata gatgagaaag
    27361 gatttaacaa tatatcagtt actgatttaa ctcaaagggc agatataaat agaggaacct
    27421 tctatctgca ttatgttgat aagtatgact tgttagagag gattgaaaat gaaataatac
    27481 aggaaattga agaggagacc aaacatcttg attcatccaa catgatggag atagatacct
    27541 caagtgaacc tcttccattt atggtaaagc tttttgaata ttttagaaaa aattcagtaa
    27601 ttattaaatc tatcttgggt cccaaaggtg accctacttt tgaaagaaaa ataaaaaaat
    27661 ttattgaaac caatttgttt gagaaacaaa aactaataaa ttttaacata gatgaggcaa
    27721 taatttcaga agaatatttt atacaatata tactttcagc agacttaggt gtaattcaat
    27781 attggcttga aaaaaatatg aaagagtccc ctgaagaaat ggcactgata cttgctagaa
    27841 tgtcgttact cggtccgtta agagcagtgg gaattcgtaa agattcataa aaatagtttt
    27901 atatatatta gactttgaaa atatatgttt aaaaaccatt ttttactact actgtttata
    27961 aattagggga gatatcttaa aatattagta aaaggagtgc taagtgagtg taataactat
    28021 gagataagag gtttagttga tatagtcaaa acttcaaaag aatcaattag aaaagttaaa
    28081 atattgcaat taaatttaaa ggagtaatat atgaaaaatg aaaatataag ttatttggcg
    28141 ttaggattat gttttggtgt tgttttgggt tcattattta aaaacttagc tattggttta
    28201 tcctttggat tagttattgg agtagtatta gacagtaaga aaaataaata aaacttcaat
    28261 agtaacatat aacaattata ctatttttgt ataaaatcat tggaaatcaa caatagaaaa
    28321 tatttgttat ttaaaaacta aaaatacagt ttaaacgacc taaaaatata ttatataatt
    28381 attataacaa gataagtgag agtaaggtta tgataataga gtaatatttt tcaattatta
    28441 ctctatttat atttatttag ttatactata gtttatattg ttattttatt ttcatatgca
    28501 gtatagggaa tggattacct tgttcatcga actcagattt tttaaagact ttaaatccca
    28561 tatgtttata aaagcctaat gcttgttgat tttgctcatt cacatctaca taatttacat
    28621 ttaatacttc tattgcatat tctacaagtc tttttccaat tccatttcct atactataat
    28681 tacttacaaa taacatttca attttaaaat catgtattcc cataaaagct tttataatac
    28741 ctttttttgt ctcttacaca taaaaaatta ctaacatatt tagcacattc tttaacttga
    28801 ggctttattg atattatatt ttcttcactt aaaaagtcat gtgttgctct tactgatgat
    28861 tcccgtgtat ctagtatttc tttcatatct ttattgttaa taatatttaa attttcaatt
    28921 tt
//
LOCUS       DAMJWP010000023        27965 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.024, whole genome shotgun sequence.
ACCESSION   DAMJWP010000023 DAMJWP010000000
VERSION     DAMJWP010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27965
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.024"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            114..1487
                     /locus_tag="PC700_000937"
     CDS             114..1487
                     /locus_tag="PC700_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2580259.1"
                     /translation="MNEILENIDEGVHFVNKDGKTIIYNNSVQKMEKMNRHDILANSF
                     FDIVNKMKINRSTLLEVLEKREVIKDNIQKYLDKNGKEITAINTTIPIDIKGEFVGAL
                     EISKNMTTIENLLNRNYKKNLVNKVSSRQKKGYEFDDILGESNLIKEAIEKSKKACKS
                     DASVFIYGETGTGKELFSQSIHYGSNRRNYPFIAQNCAALPESLFEGILFGTSKGGFT
                     GAIDRPGLFEQANKGTLLLDEINSMPIMLQAKLLRVLQEGYVRRVGGTKDIPIDVRII
                     ATTNESPKVILNEHKMRKDLYYRLNIIHIKIPPLRDRKEDILLLCKKFISKYNKKLNK
                     KVVGISDEAIKILKNYDWQGNIRELENIIYCTMSMMEDEVQIETSMINLNDYYNLKEN
                     KESNNYSLNDLEGKTLSKIISEIEEKYIYESLEKASYNITKAASILGMNRQNLQYKIK
                     KYNIKIL"
     gene            1743..2810
                     /locus_tag="PC700_000938"
     CDS             1743..2810
                     /locus_tag="PC700_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-binding protein"
                     /protein_id="HDF2580260.1"
                     /translation="MRKVRVGIWGFGAMGIGMANMILKKEGIEIVSVCSRSTSGKSMY
                     DVLGIERGERPEVIINKNYEEVFREKSVDVVLLATDSFTKKAFDKIIFLLNRKINVIS
                     TAEQMAYPQADDADLAKKMDEVAKENGVSILGTGINPGFVLDLLVLALSGTCEEVTSI
                     KAKRVNDLSPFGKSVMEEQGVGVTREEFIKGVEDKTIAGHVGFVESINMIADGLGWKL
                     DKIEQTKEPIMTTVDRKSKYGEALAGNVAGCRQCGYGYVNGDVLIEMEHPQQIIPEVE
                     GIKTGDYVSIKGIPNIDLQINPEIPGGVGTYAMIVNSIPLIINARPGLKTMLDIPVPR
                     AIMGDIRNQIEVELEEESKAN"
     gene            2907..3215
                     /locus_tag="PC700_000939"
     CDS             2907..3215
                     /locus_tag="PC700_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-ketopentanoate thiolase"
                     /protein_id="HDF2580261.1"
                     /translation="MDAKINDWVIIHNIVLTPEERAPQVPEDTKKVSLEMWVKGFIQS
                     DASIGDLVEVKTITGRLVKGNLLKVNPYYTHDYGKCIPELLQIGIQAKEILFGGVYNE
                     "
     gene            3208..4638
                     /locus_tag="PC700_000940"
     CDS             3208..4638
                     /locus_tag="PC700_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent lyase/thiolase"
                     /protein_id="HDF2580262.1"
                     /translation="MNNSTSIKDMSYQAVMGRNNEIMKNAIGLDYSSFEQEGIGFDYE
                     KMMSETGYTLQDIEAIQSQYAVGNTPLIELKNLTKLARKCAKEGKGAKIFVKDEAMNA
                     SGSFKARRAATAVYHAKQMGYKGVIAATSGNYGAAVASQAAMQGLKCIIVQECYDSNG
                     VGQPEIIEKARKCEAYGAEVVQLTVGPELFYTFLVLLEETGYFNASLYSPFGIAGVET
                     LGYELAIQFREKYKKDPDIVVCTNAGGGNLTGTARGLIKAGSINTKVVGASVDLKGLH
                     MASDNQFNKKSFTTGHTGFGIPYCTWPDRSDVPRSAARPLRYMDRYVLVKQGEVFYTT
                     ELLAQLEGIERGPAGNTSLAAAFSLAQELDEDKVIVVQETEYTGAGKHICPQLTFARE
                     NGIDIKFGNPREEIAGVNLILPERPELLKCVDIDMNKIRKSFIKNCISNNHIDNIDKL
                     SNRDIEFLMEEVKSSRDFVVDVLNNL"
     gene            4716..5090
                     /locus_tag="PC700_000941"
     CDS             4716..5090
                     /locus_tag="PC700_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HDF2580263.1"
                     /translation="MKKREDDFEVRRKHLQELSDDELKERFWNLATQIVEPMIELGKK
                     NTTPSIERSVLLRMGFSSLEVKPILEGVMERGLIGKGAGHVVYKLAKSKNITVREAGL
                     LLVKGEYWDEVTCLFREGVESC"
     gene            5084..7288
                     /locus_tag="PC700_000942"
     CDS             5084..7288
                     /locus_tag="PC700_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892628.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HDF2580264.1"
                     /translation="MLKENKKLDIDYILKDLDKYKPKRRGWVWREHLENLEMGPFKYK
                     DCTKPLKKSVGLPSSKYFNNIDPQPSPVITTEIASGRFEDDIRRMRMAAHHGADHLMV
                     IRTAGQSHFDGLIEGTPQGIGGVPITRKQVRAQRKALDFIEEEVGRPINYHSYVSGVA
                     GPEVAVMFAEEGVNGAHQDPQYNVLYRNINMVRSFVDACEAKKIMAFANIAQIDGAHN
                     ANATAREAWKVMPELMVQHALNSIFSEKIGIDKSNICLSTVPPTAPPAPCLRIDLPYA
                     VALRELFSDYKMRAQMNTKYMESSTREATVTHVLNLLTSVLTRADIQSTITPDEGRNV
                     PWHIYNIEACDTAKQALVGMDGLMDMIELKDVGELRDKARELKERAVLYMEEISEVGG
                     YFESVEQGFFVDSGNYPERNGDGISRKIKGGVGEGTVYEREEDYLAPVTAHFGYNNVA
                     QYDENAIDNPSMLIDGCTFENPDKIIYIDELDDFDNVENRLKESYEYRNGTKIKPEME
                     WCADGIVMITMMLPTDKRTAEFAALEFVKKMNLQEIEVISREVMHESEGTRIEVKGRV
                     PFDIDLNNLVIPEEPKVLTDEEIRADIEEKPMKIVSATVGEDEHSVGLREIIDIKHGG
                     IEKYGIECHYLGTSVPVEKLVDAAIELNADAILASTIISHDDIHYKNMKKLHDYCVEK
                     GIRDKVMIACGGTQVTPEIAVEQGIDAGFGRNSKGIHVATFLVEKRREMSNK"
     gene            7288..8697
                     /locus_tag="PC700_000943"
     CDS             7288..8697
                     /locus_tag="PC700_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HDF2580265.1"
                     /translation="MENSKVSLKIDVLVAEIGSTTTVVNAFHDINTNNPIFLGQGQAP
                     TTVFEGGDVRNGLSGAIKDLANKLSVDDIEYNDMFATSSAAGGLKMTVHGLVYDMTVK
                     AAKEAALGAGAVIRQITSGRIKRTDLNKIKEINPNIILIAGGVDYGERDTAIYNAEMI
                     ASMNLGIPVIYAGNVENQEEIRLIFEDTNYKLYITENVYPKIDLLNIEPTRRIIQSVF
                     EEHITTAPGMKYIKEMVNENITPTPGAVMEASKLLYKNIGDLLTLDVGGATTDVHSVT
                     DGSDYINKILVNPEPTAKRSVEGDLGVYVNMKNIVEVIGKENLQSELYIDIDAVIENY
                     PPIPKSKEEILFVERLTKEAVVKAMLRHSGKIRNIYGTTGKVKIAEGKDLTEVRYIVG
                     TGGALTRLPSRIEILDNMLKYNKNNELLFPKEKTKILIDNDYIMASMGVLSKKYEEAS
                     LKLLLKSLNFEEERLCILG"
     gene            8682..9746
                     /locus_tag="PC700_000944"
     CDS             8682..9746
                     /locus_tag="PC700_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HDF2580266.1"
                     /translation="MYPRLEIDIEKLKHNAKLISQMCHERSIKISFVTKSFCAQKEIV
                     EEICSEGIDHIADSRIQNLKKLQDINLPKILIRIPMLSELEEVINYCDISFNSELETI
                     KKLNELCESKNIIHKIVLMFDLGDLREGYFYEEDFFNNVREIVRLKNIEIIGIATNLT
                     CYGAIIPSRENIGRLVSIAKRMEEKFGINLEIVSGGNSSSIHLLINNNMPEGITNLRI
                     GESILLGRETAYGENINGTYQDAFKLICQVVECKEKPSVPIGEIGVDAFRNKPVYEDK
                     GILKRAIIAIGKQDINIDSLIPIDTDIKILGASSDHMILDVSNTKYDYKLGDNLEFLL
                     TYGGIMSSSTSKYVSKKIIV"
     gene            9776..11197
                     /gene="nhaC"
                     /locus_tag="PC700_000945"
     CDS             9776..11197
                     /gene="nhaC"
                     /locus_tag="PC700_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDF2580267.1"
                     /translation="MKERECRKASLFDAMIPITCLILFLSLGVLVYGSSPHVPLIGAA
                     AVAGLVAVYRLGFKWKELELSMFNSIKMAMQAILIIIIIGVLIGTWIVSGVVPCMIYW
                     GLKILSPNIFLVASTLVCAIVSLATGSSWSTMGTVGVALLGIGQSLGMPIGLIVGSII
                     SGAYFGDKLSPLSETTNLAPAMAGTDLFTHIKYMLYSTIPSLLICLVIYGVIGMKYSG
                     QALDIKQIELIRSTLDSTFNTLSPVLLLAPAIVIGLVVFKVPAIPGLIVGAVLGVLFA
                     VVFQGESMNTILDAAQNGYVSSTQIQEVDALLSKGGIMNMMSTVSLTICALSLGGILE
                     KTGMLEVVASSLLRLAKGVFGTVLCTMVTCTITNIIAGEQYLSIVIPGRMYNKEYKKR
                     GIHPKMLSRALEDSGTLTSPLVPWNTCGAYITATLGVSALTYGPYALLNIINPIVSLV
                     LIACKFKIARIEDEPETCLNFDV"
     gene            11626..12090
                     /locus_tag="PC700_000946"
     CDS             11626..12090
                     /locus_tag="PC700_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDF2580268.1"
                     /translation="MTRISNITIAEQSEYCFLAIRKTIDFMVEFSEFSKQSFEKISKY
                     LEERGILSSGAPIVCFHNMDLAKLDVEVGFPVATYIDGKNEILQRIVPAQKIITAIDL
                     GPYELQDPTLEDLFSWANSNGYKLHGDIYYQYLNDINRPASEYLTKMMLPII"
     gene            12388..12750
                     /locus_tag="PC700_000947"
     CDS             12388..12750
                     /locus_tag="PC700_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2580269.1"
                     /translation="MKIYDILTRFYIQDIEKAIPFYENLLKEKCSLRFSYKEVGLELA
                     QIGNVLLLSGSDDALKPFIETKSTFMVDSVDEWRSYLLDNGAVVVRDKKKVPTGYNMT
                     LRHPDGTIIEYVQHTKQD"
     gene            13345..14205
                     /locus_tag="PC700_000948"
     CDS             13345..14205
                     /locus_tag="PC700_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2580270.1"
                     /translation="MSYKVINVITAIDYIEEHLSEKLDLDIIANAVHYSKYHLHRTFT
                     TSVGLTMHDYIKRRKLTEAAKLLVFSKKPIIEIALIAGYESQQAFTSIFKAMYKKSPN
                     KYRKEQEFYPLQLRFVLKKDNLFSKNVLELEKEIKLASMSDIPLWMNLVRLVIDGFPN
                     LQEEEYIYQLEQYILEERALILKLNNVAIANMVFNRETRSIDFFGIHPQYRNSDIAQV
                     FLKKVIEDFLIDTDISITTFREGDKADTGHRDMIKKLGFAEAELLVEFGYPTQKFILP
                     CVEERDNRQN"
     gene            14463..16577
                     /locus_tag="PC700_000949"
     CDS             14463..16577
                     /locus_tag="PC700_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2580271.1"
                     /translation="MSENKQVVNCDIIASNSKHNMCEHDVELLTFFPDEIAHLIEINN
                     KLDNAFKKAENLVDKLDKDYMDAKMYMVKNRGEIDPHEMFQNEQGLKQIDNYGAFMVK
                     VRDKINKIKDSPYFARIDFRLKDMDDESKYYIGRFAFDYEDELIILDWRSPIASMFYD
                     YEIGKAGYDAPIGWVDGEITRKRQFKIKNGKLEYALESSINIQDDILQKELSHTSDEK
                     MKSIISTIQKEQNQIIRNDKADTLIIQGVAGSGKTSIALHRIAFLLYRFKDKISANNV
                     IILSPNKVFGDYISNVLPELGEEPLCELSFENIAEVQLDRVINFESEKDPLEINDAKW
                     SERVRFKSTLDFVKLIDDYIKQMPNKIFIPKDYTFGSFTAKSDWIQSRFEAYNRYPVK
                     KRLEKVAEDIHYKFESDNIMEEDLPKVKSILKSLNGMLTIKNTLTLYKDFFKQMNISN
                     MFVMAEKKTLEWSDVYPFIYIHAAYEGIQEDKIIRHIVIDEMQDYTPIQYAVINLLFK
                     CKKTILGDFGQLVNPNHTHTLDDMKQLYNDGELVMLNKSYRSTFEIINFAKKIQDISS
                     LEPIERHGEEPALLKCNNEQDEINKIKIEIEEFKKSDNATLGIILKTDNDAKAVYDAL
                     REEYSVHLISSESSSFTKGVSITSIKMSKGLEFDEVIVPSVNNKTYYSDYDRSLLYIA
                     CTRAMHKLKLTYVGTLTQLISM"
     gene            complement(16839..19082)
                     /locus_tag="PC700_000950"
     CDS             complement(16839..19082)
                     /locus_tag="PC700_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF2580272.1"
                     /translation="MSNIIIKGLTQNNLKNISFKIPKNKIVVFTGVSGSGKSSIVFDT
                     IAAESQRQMNETYSSFIRNRLPKYRKPQVELIDNLTASVIVDQTPLGGNARSTVGTIS
                     DLYSSLRLLFSRIGEPYIGTTSYFSFNDPNGMCKNCSGIGRVKVINIESIINPENSWN
                     TGCINDSLFKPNSWYWKQYAESGLFDLDKPIKDYSFEEYNLLLYGSKNKDGKQENPKI
                     EGIFNKYNQTYLNRDISNMSKHTKEKSERLITDKECPVCHGKRLNKETLNCNILGYSI
                     ADMCNMELTDLYDLLLQIKDKRTETVIKTLTDGLKRMIDIGLPYLHLNRESSSLSGGE
                     AQRLKLVRYMGSSLTGMTYIFDEPSAGMHPRDVHRMNQLLKKLRDRGNTVLVVEHDKD
                     VISIADEVIDIGPSAGTQGGHVVFQGSYDKLLLANTLTSNAMNLSLPIKTDSRHPKGT
                     LPIRDACINNLKHIDVDLPVGIMTVVTGVAGSGKSTLISRVFAQKYEKDVIKIDQSPV
                     TATNRSMPATFLGFFDEIRKVFAKENNVEEGLFSFNSKGACPVCDGKGVLVTELVFMD
                     PIINTCEACKGTRYSEEVLSKLYKGKCIVDVLSMTVDESLEFFTEKKLCKILQTMKDV
                     GLPYMTLGQPLSTLSGGERQRIKLAKNLNKKGTIYILDEPTTGLHGSDIEKLMLIFEH
                     IVDKGNTVIIIEHNLDVLKQADWVIDIGPDGGKNGGQVVFEGTPLEMFERGCTITSDF
                     LRKSINI"
     gene            19414..20130
                     /locus_tag="PC700_000951"
     CDS             19414..20130
                     /locus_tag="PC700_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580273.1"
                     /translation="MILIVILSIFIIASINIIILGKENIRDIVIPLTLFIVSDFLFLC
                     GNIICRGGVMLLFIGLFAIPLMLIAFIWICVHMIKLYYKKDLNKRKMIGCIFSIFISI
                     GTIFIPSPSESFRFKLYEKDYSVVAKAILKVYDEKKLSSESVFYIQSDKNELEKIFSK
                     EVIKKMKKLNRNLDVVKISGNRNLVYFFFGAELQSIDGIVIFKDLKHVKPKLDFINRS
                     IQAPRLEYITNNVYYFKGEL"
     gene            20158..21096
                     /gene="blaCDD"
                     /locus_tag="PC700_000952"
     CDS             20158..21096
                     /gene="blaCDD"
                     /locus_tag="PC700_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HDF2580274.1"
                     /translation="MKRKNNFIWIVILLVVVAMGVYYVGRKHNNTKQKHDKNHINSEL
                     NKSKVENNDKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETRRSPCS
                     TFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYYKKLID
                     KVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQTMAKI
                     FEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGMLEREGD
                     TYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            21440..22123
                     /locus_tag="PC700_000953"
     CDS             21440..22123
                     /locus_tag="PC700_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2580275.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            22120..24780
                     /locus_tag="PC700_000954"
     CDS             22120..24780
                     /locus_tag="PC700_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2580276.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIRLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPIAQKSKGESFKKISETTLTIVGKVDVSTSSVIPAYTALGYLDRTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKMS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYINSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKSIVSSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYDTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKTVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNTRSRRQEFAMLRSVGLSPE
                     GIKKMLVLEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(24961..26142)
                     /locus_tag="PC700_000955"
     CDS             complement(24961..26142)
                     /locus_tag="PC700_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2580277.1"
                     /translation="MKTPRNINSTVVFIIFCLIITFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVVYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(26142..26804)
                     /locus_tag="PC700_000956"
     CDS             complement(26142..26804)
                     /locus_tag="PC700_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2580278.1"
                     /translation="MAKILLLEDDDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCGNISLNKVEVKAYLDNVEVLLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DS"
     gene            complement(26949..27491)
                     /locus_tag="PC700_000957"
                     /pseudo
     CDS             complement(26949..27491)
                     /locus_tag="PC700_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(27609..27965)
                     /locus_tag="PC700_000958"
                     /pseudo
     CDS             complement(27609..27965)
                     /locus_tag="PC700_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT        10657 a         3374 c         4830 g         9104 t
ORIGIN      
        1 tcataaatat atgataagat aaatacaata gcaatatttt attaattttt tgggttttta
       61 tatatttagg tactggggtg atatagttga accaagagat attttttaaa ataatgaatg
      121 aaatattgga aaatattgat gaaggggtac attttgttaa taaagacggg aaaactataa
      181 tctacaataa ttctgtacaa aaaatggaaa aaatgaacag acacgatatt ttagccaatt
      241 cattttttga tatagtcaac aaaatgaaaa taaacaggag tacactatta gaagttttgg
      301 aaaaaaggga agttatcaaa gacaatattc aaaaatatct ggacaaaaat ggaaaagaga
      361 taacagcaat taatactaca ataccaattg atattaaagg cgagtttgta ggggcacttg
      421 aaatatcaaa gaatatgaca acgatagaaa atttactaaa cagaaattat aaaaaaaatt
      481 tagtcaataa agttagtagt agacaaaaaa agggttatga atttgatgat attttagggg
      541 aaagtaattt gattaaagaa gcaatagaaa aatctaagaa agcttgcaag tctgatgctt
      601 cagtatttat atacggtgaa actggaacgg gtaaagagtt atttagccag tccattcatt
      661 atggaagtaa taggagaaac tatccattta tagcacaaaa ttgtgcggct ttgccggaat
      721 cactattcga aggtatcctt tttggaacta gcaagggagg atttacagga gcaatagata
      781 ggccaggctt atttgaacaa gctaacaaag ggacattgtt attggatgag ataaattcca
      841 tgcctattat gttacaggct aaactattaa gggttttaca agaaggttat gtaagaaggg
      901 ttggaggaac taaggatata cctatagatg taagaattat tgcaacgaca aatgaaagtc
      961 caaaggtaat tttaaacgag cataaaatgc gaaaagattt atattatagg cttaacataa
     1021 ttcatataaa aattcctcct cttagagata gaaaagagga catacttcta ttgtgcaaaa
     1081 aatttatttc aaagtataat aaaaagttaa ataaaaaagt ggtaggaatt tcagatgaag
     1141 ctattaaaat tttaaaaaat tacgattggc aaggtaacat aagagagcta gaaaacataa
     1201 tttattgtac tatgagtatg atggaagatg aggtacaaat agaaacaagt atgattaatc
     1261 taaacgacta ttacaatctt aaagagaata aagagagtaa caattattct ttaaacgatt
     1321 tggagggcaa aacacttagt aagattatca gtgagataga agaaaagtat atatatgaat
     1381 cattagaaaa ggcatcgtac aatataacta aagctgcatc aattttaggt atgaataggc
     1441 aaaatttaca gtataaaata aaaaaatata atatcaaaat attataaata agcaagataa
     1501 aaaaacaaaa tagaagtaaa ataaaaaagc aaaatatagg agcaaaatta ttataaaaga
     1561 gcaaaatatt ttgctctttt ttttatttct cataagaaat attatttttt atttatgaaa
     1621 atacataata aaataattat agtttcaacg ttccaataaa tatttacttt aattttaaag
     1681 ttggcacgat ttatgcttta actaattatt gagaatatat aaatttttag gagggtgtat
     1741 atatgagaaa agtaagagta ggaatatggg gatttggagc tatgggaata ggtatggcta
     1801 atatgattct aaagaaagaa ggtatagaaa tagtatctgt ttgtagtaga agtacttcag
     1861 ggaaaagtat gtatgatgtt ttaggaattg aaagaggaga aagaccagaa gttattatca
     1921 ataaaaatta tgaagaagtt tttagagaaa aaagtgtaga tgttgttttg ttagcaactg
     1981 attcatttac taaaaaggct tttgataaaa taattttctt attaaataga aaaataaatg
     2041 ttatatcaac agcagaacaa atggcatatc cacaagctga tgatgcagat ttagctaaaa
     2101 aaatggatga agttgcaaag gaaaatggag taagtatact tggaacagga ataaatccag
     2161 gatttgtatt agatttacta gtattggcac tttcaggtac ttgtgaagaa gttactagta
     2221 taaaagctaa gagagtaaat gatttatctc catttggaaa atctgttatg gaggaacaag
     2281 gtgttggagt aacaagggaa gaatttataa aaggtgtaga agataagact atagcaggtc
     2341 atgtaggttt tgtagaatca ataaatatga tagctgatgg attaggttgg aaattagata
     2401 agatagaaca aacaaaagag ccaataatga ctacagtaga tagaaaatct aaatacggag
     2461 aggcattggc tggaaatgtt gctggttgta gacaatgtgg atatggatat gtaaatggag
     2521 acgttttaat tgaaatggag catccacaac agataatacc agaagtagaa ggtattaaaa
     2581 caggagacta tgtatcaata aaaggtatac caaacattga tttacaaata aatcctgaaa
     2641 tacctggagg agtaggtact tatgcaatga tagtaaactc tattccatta ataataaatg
     2701 ctagaccagg tcttaaaact atgttggata taccagtgcc tagagcaata atgggagaca
     2761 taagaaatca gatagaagtt gaattggaag aagaatcaaa ggctaattag taagataaca
     2821 gttagctaat tggtaaaata atgtttaatt taaaataaca gtaaattaat tagtaaggaa
     2881 tagtcaatta ataaggggat gaaaaaatgg atgctaaaat taatgattgg gttattatac
     2941 acaatatagt attgactcca gaagaaagag ctccacaagt tccagaggat actaagaaag
     3001 tatccttaga aatgtgggtt aaaggtttta tacaaagtga tgcttctata ggcgacttag
     3061 tagaagttaa aacaattaca ggtagacttg taaaaggtaa tttacttaaa gtaaatccat
     3121 attatactca tgattatggt aaatgtatac cagagttact ccaaatagga atccaagcta
     3181 aagaaatatt atttggaggt gtatataatg aataattcaa catcaataaa agatatgagt
     3241 tatcaagctg ttatgggaag aaataacgaa ataatgaaaa atgcaatcgg attggattat
     3301 tcttcttttg aacaagaggg aatagggttt gattatgaga aaatgatgag tgaaacagga
     3361 tatacattac aagatattga agcaattcaa tcacagtatg ctgttggaaa tactcctcta
     3421 atagagctta aaaatttgac taaattagct agaaaatgtg ctaaagaagg taaaggagct
     3481 aaaatatttg taaaagatga agcaatgaat gcatctggta gttttaaagc aagaagagca
     3541 gctactgcag tatatcatgc caaacaaatg ggatataaag gtgtaatagc agctaccagt
     3601 ggtaactatg gtgcagctgt agcatcacaa gcggcaatgc agggattaaa atgtataata
     3661 gttcaagaat gttatgattc taatggtgta ggtcaacctg aaattataga aaaagcacga
     3721 aaatgtgagg catatggagc agaagttgtt cagttaactg ttggaccaga gttattttat
     3781 acgttcttag ttttattaga agaaacaggc tattttaacg catcattata ttcaccattt
     3841 ggaattgcag gtgtagaaac tttaggttat gagttagcta tccaatttag agaaaagtat
     3901 aaaaaagacc ctgatatagt tgtttgtaca aatgcaggtg gtggtaatct tactggaact
     3961 gcaagaggat tgataaaagc tggttccatt aatactaaag tagtaggagc aagtgtagat
     4021 ttaaaaggat tacacatggc tagtgataat caatttaata aaaaatcatt tacaacagga
     4081 catacaggtt ttgggatacc atactgtaca tggcctgata ggtcagatgt acctaggtct
     4141 gcagctagac cacttagata tatggataga tatgtattag taaaacaagg agaagttttt
     4201 tatacaactg aattattggc acagctagaa ggtatagaaa gaggtccagc aggaaataca
     4261 tctttagcag cagcattctc tttagctcaa gagttagatg aggataaagt tatagtggta
     4321 caagaaacag aatatacagg cgcaggtaaa catatatgtc cacagcttac ttttgcaaga
     4381 gaaaatggaa tagatataaa atttggaaat ccaagagaag aaattgcagg agtaaactta
     4441 atattaccag aaagaccaga attattgaaa tgtgttgata ttgatatgaa taaaataaga
     4501 aagtcattta taaaaaattg catatctaat aatcacattg ataatattga taagctaagt
     4561 aatcgagata tagaattttt aatggaagaa gtaaaaagtt caagagattt tgttgttgat
     4621 gtattaaata atttatagta attattgttt ttttatttaa tataaaataa aaattcggaa
     4681 aattctatta aatcaatgcg aatggaggaa tgttcatgaa aaaaagagag gatgattttg
     4741 aagttagaag aaaacatctt caagaactga gtgatgatga gttaaaagaa aggttttgga
     4801 atttagctac ccagatagta gaaccaatga tagaacttgg taaaaagaat acaacaccat
     4861 caatagaaag gtctgtcttg ttgagaatgg gattttcttc tcttgaagta aaacctattt
     4921 tagaaggggt tatggaaaga ggtcttatag gtaaaggagc agggcatgta gtttataaat
     4981 tggctaaatc taaaaatata acggttagag aagcaggttt gttattagtt aagggtgaat
     5041 attgggatga agttacatgt ttatttagag aaggggtaga atcatgttaa aggaaaataa
     5101 aaaattggat atagactata tattaaaaga tttggataaa tataaaccaa aacgaagagg
     5161 ttgggtttgg agagaacact tggaaaatct tgaaatggga ccttttaaat ataaagactg
     5221 tacaaaacct ttaaagaaga gtgtagggct accatcatct aagtatttca ataatataga
     5281 ccctcaacca agtccagtta ttactactga aatagcttct ggtagatttg aggatgatat
     5341 aagaagaatg agaatggcag ctcatcatgg agctgaccat ctaatggtta tcagaactgc
     5401 tggacaatca cattttgatg ggcttataga aggaactcca caaggtatag gtggagttcc
     5461 aataacaaga aaacaagtaa gagctcaaag gaaggctctt gattttatag aagaagaagt
     5521 tggtagacct attaattacc attcatatgt aagtggtgtt gctggtcctg aagtagcagt
     5581 aatgtttgct gaggaaggag tcaatggagc acatcaagac ccacaatata atgttctgta
     5641 taggaatata aacatggtaa ggtcttttgt agatgcttgt gaggcaaaga aaataatggc
     5701 ttttgccaat atagctcaaa ttgatggagc acataatgct aatgctactg ctagagaagc
     5761 atggaaggta atgcctgaac ttatggttca acacgcttta aactctatat tctcagaaaa
     5821 aataggtata gataaaagta atatatgctt atcaacagtt ccaccaacag cacctccagc
     5881 accatgttta agaatagact taccttacgc tgttgcactt agagaattat ttagtgatta
     5941 taagatgaga gctcaaatga atactaaata tatggaatca tcaactagag aagcaacagt
     6001 tactcatgta ttaaacttat taacatcagt attaactaga gctgacatac aatcaactat
     6061 aacacctgat gaaggtagaa atgtaccttg gcatatatac aatatagaag catgtgatac
     6121 agcaaagcaa gctttagttg gtatggatgg gcttatggat atgatagaac ttaaagatgt
     6181 cggtgaatta agagacaaag caagagaact taaagaaaga gctgtattat atatggaaga
     6241 aatatctgaa gttggtggat attttgaatc tgtggaacaa ggattttttg ttgattctgg
     6301 aaattatcca gaaagaaatg gtgatggaat atctagaaaa ataaaaggtg gagttggtga
     6361 aggtactgtt tatgaaagag aagaagatta tttagcacct gtaactgctc actttggata
     6421 caataatgta gctcaatatg atgaaaatgc tatagataat ccatcaatgt taatagatgg
     6481 atgtacattt gaaaatccag acaaaataat atatattgat gagcttgacg attttgataa
     6541 tgtagaaaat agactaaaag aatcatatga gtacagaaat ggaactaaaa ttaagcctga
     6601 aatggaatgg tgtgcagatg gaattgtaat gataacaatg atgcttccta ctgataaaag
     6661 aacagcagag tttgcagctt tggaatttgt caaaaaaatg aacttacaag aaattgaggt
     6721 cataagtaga gaagttatgc atgaatctga agggactaga atagaagtga aaggaagagt
     6781 tccgtttgat atagatttaa acaatttagt aataccagaa gaacctaagg tactaactga
     6841 tgaagaaata agagcagata tagaagaaaa acctatgaaa atagtttcag ctacggttgg
     6901 agaagatgaa cattcagttg gtttaagaga aataatagat ataaaacatg gaggtattga
     6961 aaaatatggc atagaatgcc actatctagg gacttctgta cctgtagaaa aattggtaga
     7021 tgcagcgatt gaactgaatg cagatgcaat acttgcatct acaataataa gtcatgatga
     7081 tattcactat aaaaacatga aaaaattaca cgattattgt gtagaaaaag gtataagaga
     7141 taaagtaatg attgcgtgtg gaggaacaca agtaacacct gaaatagctg ttgaacaagg
     7201 catcgatgct ggatttggaa gaaacagcaa aggcatacat gtagccactt tcttagttga
     7261 aaaaagaaga gaaatgagta ataaataatg gaaaatagca aagtttcttt aaaaatagat
     7321 gtgttggtag ctgaaattgg aagtactact acagtggtaa acgctttcca cgatattaat
     7381 actaacaatc ctatattttt gggtcaaggt caagctccaa caacagtgtt tgaaggtggc
     7441 gatgtaagaa atggcttatc aggtgctatt aaagatttag ctaataaatt atctgtagat
     7501 gatatagagt ataatgatat gtttgcaact tctagtgcag ctggtggatt aaaaatgact
     7561 gttcatggat tagtctatga tatgacagta aaagcagcta aagaagcagc tttaggggct
     7621 ggagctgtta ttcgtcaaat aacatcaggt agaattaaaa gaactgattt aaataaaata
     7681 aaagaaataa acccaaacat aattttaata gctggtggag tcgattatgg agaaagagat
     7741 acagctattt ataatgcaga aatgatagct tctatgaatc taggcatacc agttatatat
     7801 gctggtaatg ttgaaaatca agaggaaata aggttaatat ttgaagatac taattataag
     7861 ctttatataa ctgaaaatgt ataccctaaa atagatttat tgaatataga acctacaaga
     7921 agaatcattc aaagtgtatt tgaagaacac ataactacag caccaggaat gaaatacatt
     7981 aaagaaatgg ttaatgaaaa tattactcca accccaggag ctgtaatgga agcttctaaa
     8041 cttttatata aaaatattgg agatttatta actttggatg taggtggagc aactactgat
     8101 gtacattctg ttacagatgg aagtgactat attaataaaa ttttggttaa tcctgaacct
     8161 actgcaaaga gaagtgttga gggggattta ggtgtatatg taaatatgaa gaatatagta
     8221 gaagttatag gtaaagaaaa cttacaaagt gaactatata tagatattga tgcagtaata
     8281 gagaattatc ctccaatacc aaaatcaaaa gaagaaatat tatttgttga gaggttgaca
     8341 aaagaagctg ttgttaaagc tatgctaaga cattcaggaa aaataagaaa tatctatgga
     8401 acaactggaa aagtcaaaat tgcagaaggg aaagatttaa cagaagtaag atatatagtt
     8461 ggtacagggg gagcacttac taggctccct agtagaattg aaatattaga taatatgctc
     8521 aaatacaata aaaataatga attactattt ccaaaggaaa aaacaaagat attaatagat
     8581 aatgactata ttatggcatc tatgggtgtt ttatctaaaa aatatgaaga agcatctctt
     8641 aagctcctgt taaaaagttt gaattttgag gaggaaagat tatgtatcct aggttagaaa
     8701 tagatataga aaagttaaag cataatgcaa aattaatttc gcagatgtgt catgaaagaa
     8761 gtattaagat atcttttgtt actaaatcat tttgtgcaca aaaagaaata gtagaagaaa
     8821 tatgtagtga aggaatagac catatagcag attcaagaat acaaaactta aaaaaattac
     8881 aagatataaa tctacctaag atattgatta gaatcccaat gttaagtgaa ttagaagaag
     8941 ttatcaatta ttgcgatatt agttttaact cagaattaga aacaataaag aaattaaatg
     9001 aattatgtga aagtaaaaat attattcata agatagttct tatgtttgac ttaggcgatt
     9061 tacgagaagg atacttttat gaagaagact tttttaataa tgtaagagag atagtaagat
     9121 taaaaaatat tgaaataatt ggtatagcta caaacttaac ctgttatgga gctattatac
     9181 catctaggga aaatataggc agactggtaa gtatagcaaa aagaatggaa gaaaaatttg
     9241 gaataaatct agaaatagta tctggaggta attcaagttc tattcatctg ttgataaata
     9301 ataatatgcc agaaggtatt actaatttga gaataggcga gtccattcta cttggtagag
     9361 aaacggcata cggtgaaaat ataaatggaa catatcaaga tgcatttaaa ttaatatgcc
     9421 aagtagttga atgtaaagaa aaaccatcag ttcctattgg agaaatagga gttgatgcct
     9481 ttagaaataa acctgtttat gaagataagg gaatcttaaa aagggcaatc atagctatag
     9541 gtaaacaaga tataaatatt gactctttaa ttccaataga cactgatata aaaatattag
     9601 gggcaagttc agaccacatg atactagatg taagtaacac aaaatatgat tataaattag
     9661 gtgacaattt agaattttta ttaacttatg ggggtatcat gtcatctagt acaagcaaat
     9721 atgtttcaaa aaaaattata gtgtagtgtt taaataatta ggaggaaact attttatgaa
     9781 agagcgagaa tgtagaaaag caagtctgtt tgatgcaatg ataccaatta cttgtttgat
     9841 attattttta agtctaggag tattagtgta tggttcatca cctcatgtac cactaatagg
     9901 agcagcagca gtagcaggac ttgtggctgt atatagactt ggatttaaat ggaaagagtt
     9961 agaattaagt atgtttaata gtataaagat ggcaatgcaa gcaatcttaa tcataattat
    10021 aataggcgta cttataggaa cttggatagt aagtggagta gttccatgta tgatttactg
    10081 gggactaaaa attttatcac caaatatttt tttagtagca tcaacattag tctgtgcaat
    10141 agtatctctt gcgacaggtt catcttggag cacaatgggt acagtaggag tagctctttt
    10201 aggaattggt caaagtttag gtatgccaat tggattaatc gtaggttcta taatatctgg
    10261 agcttacttt ggagataaac tatcaccttt atctgaaacg actaacttag ctccagcaat
    10321 ggcaggaaca gatttgttta cacatataaa atatatgtta tattcgacta taccatcatt
    10381 attaatttgt ttagtaattt atggtgtaat tggaatgaag tattcaggtc aagcactaga
    10441 tataaaacaa atagaattaa ttaggtctac attagattct acatttaata ctttatcacc
    10501 agtattatta ttagctccag caatagtaat tggattagtt gtatttaaag ttcctgctat
    10561 accagggctt atagtaggtg ctgtattggg agtattgttt gcagtagttt ttcaaggaga
    10621 atctatgaat acgatattag atgcagcaca aaatggatat gtttcgtcaa cacaaataca
    10681 ggaggtagat gctttattat ctaaaggtgg gattatgaat atgatgtcca ctgtatcctt
    10741 aacaatatgc gctttatctc ttggaggtat tttagaaaaa acaggtatgc ttgaagtagt
    10801 agcatcttct ttactaagat tagcaaaggg tgtatttgga acagtattat gtactatggt
    10861 aacttgtaca attaccaata taatagcagg tgaacaatat ctatctatag ttataccagg
    10921 aagaatgtat aataaggaat ataaaaagag aggtatacat cctaagatgt tgtcgagagc
    10981 tttagaagat agcggaactt taacttctcc attagtacca tggaatacat gcggggcgta
    11041 tataacagca acattaggtg tatctgcact aacatatggt ccatacgctt tattaaatat
    11101 tataaatcca atagtatctt tagtattaat tgcatgtaag tttaaaatag caagaataga
    11161 agatgaacct gaaacttgtt taaattttga tgtttgataa tagataactt atgtaagaac
    11221 gtaggaacta gggggggagc ctgtggggaa ttagtaagac atattttagt gtgcgaaatt
    11281 ctataaatca gtaatcttgt gactttagcc atgggaggtt cagattagta aatttatagt
    11341 tttatattag tctatacttt gatcttttaa ttgtttgtat aaatttttta tagaaaaaga
    11401 tttcttttag acttgcctaa aaattaggca agtcttttaa gtatgttctg attataagag
    11461 cactgtgtga caagaatgat gagattattt ttgctatact ttcgatataa gaaatggaag
    11521 gaggttatgt tttatattta tatgaaataa ttagttacac agtgaatttt cgtataattt
    11581 ggtataaaaa gattagaaat gaaaataaag taatggagga ataaaatgac tagaatatcc
    11641 aatataacaa tagctgaaca atctgagtac tgttttttgg caatacgaaa aacaattgac
    11701 tttatggtag agttttcaga gttttcaaaa caaagttttg aaaaaatttc taaatatctg
    11761 gaggagagag gaattttatc ttcaggagca ccgattgtat gttttcataa catggattta
    11821 gcaaaattgg atgttgaagt agggtttcct gtagccactt atatagatgg gaaaaatgag
    11881 attctacaaa ggattgttcc tgcacagaaa ataataactg caattgattt aggaccttat
    11941 gaattacaag accctacttt agaagactta ttttcatggg caaatagcaa tggatataaa
    12001 cttcatggcg atatatatta ccagtattta aatgatatca atcgtccagc tagtgaatat
    12061 cttacgaaaa tgatgttacc cattatatag aaagttaaag attatcgttt gttagttgat
    12121 aattagaaga acatttaatg ctactgcata aattttatcc taaataatat caaggtgatt
    12181 taatttatgt attctaatgt aactgtagat aatcaattta taactaactt agtgtaattt
    12241 agatttaatt tattattcta atgtaacccc aatatattgt taaggtctac ttaagatgct
    12301 caaaaggcta tgcttaattt attgttatat aatttgtaga gtggtataat ttagaagtga
    12361 tactatatag atatataggg ggaaaatatg aaaatatatg atatattaac acgtttttat
    12421 attcaagata tagaaaaagc catacctttt tatgagaatc ttttaaaaga aaaatgttct
    12481 ttgcgttttt cttataaaga agttgggttg gaattagcgc aaataggaaa tgttttacta
    12541 ttatctggtt ctgatgatgc tcttaaacca tttatagaaa caaaatctac ttttatggtt
    12601 gattctgttg atgaatggag aagttatcta ttggataatg gagcagttgt agtaagagat
    12661 aagaaaaaag ttcctacagg atataatatg acattgagac atccagatgg tacaattata
    12721 gaatatgttc aacatacaaa gcaggattag ttaaaaatag attttactac tgagcaacta
    12781 attagaatct attcttagac gtacaattta ttatttttgg tattaataga ttataagtca
    12841 aattataata tttcaaaata gaatagaaat ttatttacat aaaagagagt gtttaaaaat
    12901 gaacttttta gttcacgaag cactcctttt tgtatgaaat caaatacatc aatatataaa
    12961 acaaactgat gaagagctta aattaaaata aaaagattca taacaggacc ccattaaact
    13021 ttaagatagg gtaagtttgt aatcatatta aataattgta aaaagctgtt tttagtcgta
    13081 taatataaat atttatcatt taattaagga atctaaaagg aatctaataa gaagaaaata
    13141 ataaataact ttttagattt actaaatgaa aaattattga aaattaaagg attagagtct
    13201 ttaatcagaa agtaaaaagt aatatattaa atttatgaat tgaaaactta aacaacaaat
    13261 ttgtttataa aatagcaaga ttcattaaat ataactaagt aataattgat ataatgttgc
    13321 atggataaaa ggagggaagc tgcaatgtct tacaaagtaa ttaatgtcat aactgctatt
    13381 gactatatag aggagcattt atcagaaaag ctggatttag atataattgc taatgctgtt
    13441 cattactcaa aatatcacct gcatagaact ttcacaactt ctgttggtct aacaatgcat
    13501 gattacataa agcggagaaa gttgacagaa gcagcaaaat tattggtatt ttcaaagaaa
    13561 ccaattattg aaattgcatt gatagctgga tatgaaagtc aacaggcatt cacttctata
    13621 tttaaagcta tgtataaaaa atctccaaat aaatatagga aagaacaaga attttatcct
    13681 ttgcagctaa gatttgtttt gaaaaaagat aacctatttt caaagaatgt actagagttg
    13741 gaaaaagaaa ttaaacttgc aagtatgtct gacataccat tatggatgaa tttagtgaga
    13801 ttagtcatag atggatttcc aaatttgcaa gaggaagaat atatatatca attagaacag
    13861 tatattttag aagaaagagc tttaatatta aaacttaata atgtcgctat tgcaaatatg
    13921 gtgtttaata gagaaactag aagtattgat ttttttggaa tacatccaca gtatcgtaat
    13981 tcagacattg cacaagtgtt tttgaagaaa gttattgaag atttcttgat agatacagat
    14041 atctcaatta ctacctttcg agagggtgac aaagctgata caggacatag agatatgata
    14101 aagaagttgg ggtttgcaga agcagagttg cttgtggaat ttggatatcc tactcaaaaa
    14161 tttattcttc cttgtgtaga agaaagagat aatagacaaa attaattgta gattaaatgc
    14221 aaagtttacc acatattttt tgtttgaatt attttatata taagagtatt tctgctttta
    14281 atgtattttg cttgtaagtg tacttatggt aataaatata gaaagattta gttttttaaa
    14341 aaaagaactt taaagctaaa aagctaacat ttaagttaaa tagcaagaat gattaaaaac
    14401 tccaaaacac aattaagtat aatgattcag tgtaagtact taattaacaa aggaggattt
    14461 aaatgtctga aaataaacaa gtagtaaatt gcgatattat tgcatccaat tctaaacata
    14521 atatgtgtga acatgatgta gagttattaa ctttttttcc agatgagatt gcacatctaa
    14581 tagagataaa taacaagcta gacaatgctt ttaagaaagc tgaaaatctt gttgacaagc
    14641 ttgataagga ttatatggac gctaaaatgt atatggtaaa aaatcgtggt gaaatagacc
    14701 cacatgaaat gtttcaaaat gaacaaggac taaaacaaat agataactat ggggctttta
    14761 tggtaaaagt tcgagataag attaataaaa tcaaagattc tccatacttt gctagaatag
    14821 attttaggct gaaagatatg gatgatgaat ctaagtatta tattggtaga tttgcatttg
    14881 attatgaaga tgagcttata atacttgact ggcgttctcc aattgccagt atgttttatg
    14941 attatgaaat cggaaaagct ggatatgatg ctccaatagg atgggtggat ggagaaataa
    15001 caagaaaacg acaatttaaa attaaaaatg gaaaattgga atatgcacta gaaagttcca
    15061 ttaatataca agatgatatt cttcaaaaag aacttagtca cacttcagat gaaaaaatga
    15121 agtcaatcat ttcaacaata caaaaggaac aaaatcaaat tattagaaat gataaagctg
    15181 atacactgat tattcaaggg gtagctggtt ctgggaaaac atctatagca ttacacagaa
    15241 ttgcttttct tctttatcga tttaaagata aaatatcagc aaataatgta attatcctat
    15301 cacctaataa agtctttgga gactatattt ccaatgtcct tcctgagctt ggagaagaac
    15361 cattatgtga attaagtttt gaaaatatag cagaggtaca attagataga gttattaatt
    15421 ttgaaagtga gaaagaccca ttagaaataa atgatgctaa atggtctgaa agagtgcgtt
    15481 ttaaatctac acttgatttt gtaaaactta ttgatgatta tataaaacaa atgccaaata
    15541 agatttttat tccaaaagac tacacttttg gaagttttac agctaaaagt gactggatac
    15601 agagtagatt tgaggcatat aacagatatc cagttaagaa gagattagaa aaggtagctg
    15661 aagacattca ttataaattt gaatcagata atattatgga agaagattta ccaaaggtaa
    15721 aaagtatact taaaagtttg aatggaatgt tgacaataaa aaatactctt acactatata
    15781 aggatttctt taaacaaatg aatatatcaa acatgtttgt gatggcagaa aagaaaacac
    15841 ttgaatggtc agatgtatac ccatttatat atattcatgc tgcatatgaa ggtatacaag
    15901 aagataagat tataaggcat attgttattg atgaaatgca ggactataca cctattcaat
    15961 atgctgtaat aaacttactt tttaagtgta aaaagactat tcttggagat tttggacaat
    16021 tggttaatcc taatcatact catacattag atgatatgaa acagctatat aatgatgggg
    16081 aactagttat gttgaacaag agttaccgtt ctacttttga gattattaat ttcgccaaaa
    16141 agattcaaga tatatcttca ttagagccta ttgaacgaca tggtgaagaa ccggctttat
    16201 taaaatgtaa taacgagcaa gatgaaataa ataagataaa aatagaaatt gaagaattta
    16261 aaaagagtga taatgcgact cttggtatta tattaaagac agataatgac gctaaagctg
    16321 tatatgatgc gttaagagaa gaatatagtg ttcacttaat atcttctgaa agttctagct
    16381 ttacaaaagg agtatccata acttctatta aaatgtcaaa aggtttagag tttgatgaag
    16441 ttattgtacc ttcagtaaat aataaaacat attatagtga ttatgaccgc agtttgttat
    16501 atattgcttg tacacgagct atgcacaaac ttaagttaac atatgttggt acattaactc
    16561 aacttataag catgtagatt atatcttata tgcatttatt atcgtaaata taaattggga
    16621 caaagattca tcaataaatc aacaaaaaat aatacttata aatttataaa aaatgaaagc
    16681 tataaattac atttgaaatt ttataaaatt ttgtgattta tagcttttat aatattaatt
    16741 tcttttaaaa taaagttaaa ctttaaattt atgaaataaa acaaagtagt aatatatatg
    16801 taatagttct taatttttta atattagtat tatattatct atatatttat tgattttcta
    16861 aggaaatctg atgttatagt gcaacctctt tcaaacattt ctagtggtgt accttcaaat
    16921 acgacctgac caccgttttt gccaccatca ggaccaatat caattaccca gtctgcttgt
    16981 ttcaatacat ccaaattatg ttcaattata attacagtat tacctttatc aactatatgc
    17041 tcaaaaatca acataagctt ttcaatatca gaaccatgaa gccctgtagt tggctcatct
    17101 aaaatatata tagtaccttt tttgttgagg tttttagcta acttaattcg ttgacgttct
    17161 cctccagata gtgtgctaag tggttgacct aaggtcatat aaggtaaacc aacatctttc
    17221 attgtttgta agattttaca tagcttcttt tctgtaaaaa attcaagaga ttcatctact
    17281 gtcatagata gtacatcaac tatacattta cctttatata attttgacaa aacttcttca
    17341 ctatatctag tacctttgca agcttcacat gtattaataa tagggtccat gaatacaagc
    17401 tctgtaacaa gtactccttt accatcacaa acaggacaag ctccctttga attaaagctg
    17461 aataaacctt cttcaacatt attttcttta gcaaatactt ttcttatctc atcaaagaaa
    17521 ccaagaaatg tagcaggcat agaacgattt gtagctgtaa ctgggctttg gtcgatttta
    17581 ataacatctt tttcgtattt ttgtgcaaaa acacgagaga ttaaagtact cttgccagaa
    17641 cctgctactc cagtaacgac agtcataatt ccaactggta aatctacatc aatatgtttt
    17701 agattattta tgcaagcatc tctaattgga agggtacctt ttggatgacg agagtcagtt
    17761 tttataggta atgatagatt cattgcattt gaagtaagag tgtttgctaa aagtaattta
    17821 tcataagagc cttggaatac aacatgtcca ccctgagtac cagcacttgg gccaatatct
    17881 ataacttcat ctgctatgga gataacatct ttatcatgct ctactacaag gacagtattt
    17941 cctctatctc taagtttttt taatagctga ttcatacgat gtacatctcg tggatgcata
    18001 ccagcactag gttcatcgaa aatataagtc atacctgtga gactgctccc catatagcgt
    18061 acaagtttta atctttgagc ttctcctcca gatagagatg atgattctct atttagatgt
    18121 aggtaaggga gaccaatatc aatcattctt tttaaaccat cagtaagagt ctttattaca
    18181 gtttcagttc ttttatcttt tatttgtaac aataaatcat aaaggtcagt aagttccata
    18241 ttgcacatat ctgcaatcga atatcctaaa atattacagt taagagtttc tttatttaat
    18301 ctttttccat gacatacagg acattcctta tctgttataa gtctttctga tttttctttt
    18361 gtgtgtttgc tcatattgct aatatctcga tttagatatg tttgattgta tttgttaaaa
    18421 ataccctcta tttttggatt ttcttgtttt ccatctttat tttttgaacc ataaagcaat
    18481 aaattatatt cttcaaaaga ataatctttt ataggtttat ctaaatcaaa aagtccagac
    18541 tctgcatact gcttccagta ccatgaatta ggtttaaata aagagtcatt tatacatcct
    18601 gtattccatg aattttcagg atttataata gattcaatat tgattacttt aactctacca
    18661 atcccagaac aatttttaca cataccattt gggtcattaa aagaaaaata tgaagttgtt
    18721 cctatgtagg gttctccaat tctagaaaat aataatctta atgatgaata caaatcactt
    18781 atagtgccaa ctgtagaacg tgcattgcca ccaagaggag tttggtctac gattacagaa
    18841 gcagttaaat tatcaatcag ctccacctga ggttttctat atttaggtag cctatttctg
    18901 ataaatgaag aatatgtttc attcatctgc ctttgtgatt ctgctgcaat tgtatcaaat
    18961 acgatactgg attttccaga accagaaact ccagtaaaaa caacaatttt atttttagga
    19021 attttaaatg atatattttt taggttattt tgtgtaagtc cttttattat aatatttgac
    19081 atgaatgtta aatcctttct gagtacattt tagtaaagca attatattat gtaaccatat
    19141 tataataaaa ttaaaatcta ctttcttgat atattctgct atgttataga aggtatttct
    19201 aaatttaacc aatcccatac aatctgaata aatgatagtt gataatagat aaaaaagtat
    19261 ataatagata ctcgtaattt gataagtaca aagataatta tacaattaaa acgacttgac
    19321 atttaaaact tactattgta atatttaaat attacaatag taagttttaa atagagaata
    19381 aataattacc tcacaaactg aaaggaggac ataatgatac taatagttat attgagtata
    19441 ttcatcatag cctcaatcaa cataattata ttaggtaaag aaaatataag agatatagtt
    19501 atccctttaa ctctatttat tgtaagtgat tttttatttt tgtgtggaaa tataatatgt
    19561 aggggaggag taatgctact atttataggt ttatttgcaa taccattgat gctaattgca
    19621 tttatatgga tttgtgtaca tatgattaag ctttactata aaaaagattt aaataaaaga
    19681 aaaatgattg gatgtatatt tagtatcttt ataagtatag gtactatatt tattcctagt
    19741 ccaagtgaaa gttttagatt taaactttat gaaaaagatt atagtgtagt agctaaagct
    19801 attttgaaag tttatgatga aaaaaagtta agctctgaat ctgtctttta tattcaatct
    19861 gataaaaatg aattggagaa gatattttct aaagaagtaa taaaaaaaat gaaaaaacta
    19921 aacagaaatt tagatgtagt taaaatttct gggaatagaa atttagtcta ttttttcttt
    19981 ggagctgaat tacaatctat tgatggtatt gtgattttta aagatttaaa acatgttaaa
    20041 ccaaagttag attttataaa tagaagtatt caagctccta gattggagta tattacaaat
    20101 aatgtatatt attttaaagg tgaactataa acttatgtag aggagatggt ataaagtatg
    20161 aaaagaaaaa ataattttat ttggatagtt attcttctag ttgttgtagc tatgggagta
    20221 tattatgtag gaagaaagca taacaacaca aaacaaaaac atgataagaa tcatataaac
    20281 tctgaattaa ataaatccaa agttgaaaac aatgataaaa acaaaaaaga aaaaattaat
    20341 atggttgact atagtgactg ttttgaaggt attagtggag gagctatttt ttataatact
    20401 aaaaataaag aatataatat atataacaaa gaattaattg aaacaagaag gtcaccatgt
    20461 tctactttta aaatagtttc aactttaatt ggtttagaaa aaggggtaat aaactcaaaa
    20521 gaatcagtta tgggatatga tggtacagag tatccaaata aaaattggaa taaaaatctt
    20581 agtttagaag aggcatttaa ggagtcttgt gtttggtact acaaaaaatt gatagataaa
    20641 gttgatgcta aatctgtcca aaacatttta gatgatttaa agtatgggaa ttgcgatatt
    20701 agcgaatggg aaggagattt aaaaaatgga aagggacatt taaatggatt ttggttagaa
    20761 tcttcattac aaatatctcc aaaagaacaa gtgcaaacaa tggcaaaaat atttgaggga
    20821 gatacaaact ttaaaaaaga gcatattaat attttaagag atattatgaa aatagatgta
    20881 aatgataaaa acataaatgt atatggaaaa actggaacag gatttgatga aaaaaataaa
    20941 cgtgtggatg catggtttgt tggtatgcta gagcgtgaag gtgacaccta ttattttgct
    21001 attaaatcag atgattccaa taaagaaatt acagggccaa aggtaaagga aattgcaatt
    21061 aatataatta agaaatatta tagtgtaagg gaataaactt aattttattt gtaaatatta
    21121 tttacctata gtctgtaata tttattaaat aattttacta aattgagtct atatagagca
    21181 aaactccttt agtacataaa atacttgcta ttgattatta aattgaatac ctatagattt
    21241 aaagttaata ccttttaaag actatgtaat atattaaaag tataaataaa atatattgca
    21301 tagtttttgt tttatattat aattttaaat aatgtgcaaa aactaaacaa tatttttaat
    21361 agtaatttat atttgtattc atgtcactat aatgtcattt taggttgcta aaatttaacc
    21421 ataaaggaga tgattgtata tggaaatatt aaaatgtgaa aatctcacaa aaatatatgg
    21481 ttcaaatcaa accagagtga cagcccttaa taatgtaaat ttatcagttc aaaaaggtga
    21541 ttttgtatcc attgtaggag catctggttc tggcaaatct acattgttac atctgcttgg
    21601 gggagtagat agacctacat ctggaaagat ttatgttgaa gatacagaaa tatcaagttt
    21661 aaaggaagaa gctcttgcag tttttagacg tagaaaagta ggtcttattt atcaatttta
    21721 taatcttata cctacccttg atgttagaaa aaatatatta ttacccatgt tacttgataa
    21781 aagaaaggtg gatgaggata gattctctga gattgtatct atattgggat tatctgatag
    21841 attaaaccat cttccaagtc aactttcagg tggacaacaa caaagggtat ctatagctag
    21901 aagtttaatc tatagaccag ctattttatt agcagatgag ccaactggta atcttgatag
    21961 aaaaaattca gaagaaattg tagacttgtt aaacttatca aataaacgat ttaatcagac
    22021 aatacttctt attactcatg atgagaaaat agcactagag gcaaatcgta ttgtaactat
    22081 ggaagatgga gtgattgtct ctgaaaaggt ggtgaagaaa tgaatattct tcaagaatac
    22141 accttagact ttgtacgacg caataaacgt agtagtatag ctataatgtt agctatatta
    22201 cttactacaa ccatgatgtc ttctttgtgt ggactgttgt ataccatgtg gacagatttt
    22261 ataagacttt caattgagaa agaaggtaac tggcatggtg aattgtttga ctatacttat
    22321 ggaagtgact taccattaat tgaaaatttt tcttcagttg atgatgtaat ggtaaaagga
    22381 caatggtatg taggcaaaat taatgatgag aaaagagatt atataattta tagaaatgct
    22441 acatcagagt attggaattc tatgccagaa aaggatacta taatagaggg tcgtcgacca
    22501 aataatgcta atgaaattgc attatcaaaa caatattttg agaataatcc aagtgtaaaa
    22561 attggggata aaattactct tccaatagga gatagaatcc taaatggaaa gcctcttgaa
    22621 cccatagctc aaaaatcgaa aggggaaagt tttaaaaaaa tttcagagac tacattgaca
    22681 atagtaggaa aggtagatgt ttccacttca tcagtaatac ctgcatatac agctcttgga
    22741 tatcttgata gaacaagtat aaaacctaat gatagtataa ctgtgtactt gcgatttaaa
    22801 aatataagag acacatacaa ggaacttcca aaacttgcta aatcattggg gtggaagact
    22861 aatgaatatg gaaaatacaa tcttaaatat aatagtaatt atcttttaaa aatgttagtc
    22921 ttttccccag aacagaaagc atctatgagc agtatatcaa aattttcaac tcctattatg
    22981 tatcttacaa tagcaatttt tacagtagct gtgtttgtaa tggtgattta caacgcgttt
    23041 tcactatctg caaatgctag acttactcaa ttagggatat tatctagtgt tggtgcatca
    23101 ccaaaacaga ttaaaatgtc tgtagtgttt gaaggttttt tgcttacaat tataccattg
    23161 ccaataggtt tatttttagg atggttattg tgtaataggc ttatagttta tattaattca
    23221 gttaattatc atactgatgt tccagaagtg gtatttactt atggtattcc tgcttttctg
    23281 ccagcagttt tacttacaat agtaacagtc tggatttctg cacttatacc agcacgaaaa
    23341 gtatcaaaga tatctcctat agaagcaata cgacaaggag atagtgttaa aatcaaaaag
    23401 tcgcataaat attcattagg aaagatattt ggaatagaag gagaacttgc atcaaacgca
    23461 ctacgtgctc gtaaaaaatc ttatcgtact gcaacaatat ctttaacatt atctttttta
    23521 ttacttacat gttttttgca tataattgca aatcaagaag gagcaaaagc tgtttatgga
    23581 agtgataatt ataaggacca aagagatata gcagtttatt tggaaaatag tgaacctgta
    23641 gagtctgctt tcattgaaaa attagataat acagaaggtg taaaaagttc actatattat
    23701 caaaaaatgt ctgcttccac ttggctgact gaaaaagatg catcaaaaga acttgcgaaa
    23761 aatggaggat ttaaaagtat agtttcatct aaaaaatact ctcctataga acgtgatggc
    23821 aagtttcgta taatttcaaa tataattggg cttgatgata agagttttac agaatattgt
    23881 aggcaaattt ctgtagaccc aaagttattt tatgatacaa aaaatccaac ctcaattgta
    23941 tataataagg aggaagatat taatcgtagt acacgtagaa ataaagtata tatagattat
    24001 ttaaatcttt caataggaga taacattaaa ttaaatgaaa aagtatatga cgaagataaa
    24061 ggggattata cttacaacat gaaagtggga gctattacaa gcacacttcc taaaacagta
    24121 gatactagtt caaaatatac attaatgcaa atagtgccaa tgtctatggt gcaggaaata
    24181 tctaaaaatt atgatgaagt aagtaggtta cgttcaaata ggctgatagg agtatttaaa
    24241 gtaaataatc gtgaggacat tcctagagta agagaaaaat tagagtccat ttataataaa
    24301 gactatggtt caggagatta tagtattgag gatgttttag aaagtgaaaa acaaaaggct
    24361 agtggaataa agaccatgaa tttaatagta ggctttatta caggtttatt ggctatgatt
    24421 ggattatcaa atgtacttgc tacagtgtca ggaaatacta gaagtagacg acaagagttt
    24481 gctatgttgc gttctgttgg attatctcca gaaggcataa aaaagatgct tgttttggaa
    24541 ggtttgttct taggaattac accattacta cttagtattc ctgtgcaaat aggaatagtg
    24601 tatgcttttc tacgtataaa tgaaatatat tttatagagt atttaccatt tgcacctata
    24661 tctacaataa taggttttac tatattgata ctttttattg taatagcatc atatatgaca
    24721 ggatacaagc agttgaagaa tgaaaatata gtagaatcta taaaaaatga aacaatataa
    24781 acaaaagcta tttgaactta tttaaaggtt tcgatttaag actacttaca agtataggga
    24841 tagttaattt gtttgtttaa taaaacctta aatgtaaata gaagtatatt actaaaacaa
    24901 tagataacat agaatataga tatagctgga ttataggtat tttacctata atccagcatt
    24961 ttatgtgtaa aatttaatta tgaatcttgc accaccatta tggcaatttt ctgcatgtag
    25021 agtaccattt tgacgttcta taatagactt tgatagagca agccctatac caatactgtc
    25081 cttactagca tttttaccac gataaaatct attaaaaata tatggaagtt cttcctgagc
    25141 aaacccttta ccattatctt ctatgataat ctcaacatat agaggatttt gactataata
    25201 agcaactata tatccaccat cttctatatg ctcactacaa tttttaagaa tatttaaaag
    25261 agcttctaaa gtccaattaa tatcgatttc aaattctacc gaaggagaag cctcgatagt
    25321 aaatgtctga tgtttttctc ttaaagaacc ttctataggc tcaattgcat aagtaagaag
    25381 agaatgtaca tctataagtt tgctttcaaa tattaaagta cttgcatcca attttgataa
    25441 tgtaagtaaa gatgaaacta gtctctcaag tcttgagatt tgatttgtaa gtctgttaat
    25501 ataatctatt tcttcatctg gacaattttc tgaaagtaat tgtgccataa gtgacataga
    25561 agtaattgga gtcttaagtt gatgagcaat atctgcaaga ttatctgaaa gagtttgtct
    25621 ttcttttaaa gcaaattctt ttgaacaacg aagttcccca actgttttat atatctcatc
    25681 ttcaaggtga gaaaattcat cttcacatcg taaaagtaca gactcttttc ctaaattcac
    25741 ttcttctaag tattgagtaa gattgtttat tctactcagt ttttgcttat ttgttctatg
    25801 tttgattata tatacaactg tagccattgt tgaaattata ccaaacgata tcactagaaa
    25861 ataaaaaaag tttttactcc aaaatgtact tttagaatat ccatgttttg ataaaatttc
    25921 atttcctgtt ttaaaatcct tatcactaga atttttcagt gattttagta tactttctgt
    25981 agacttagga ttgtactcat atgatgcacc aagagcctta gacagtacat tgtattcatt
    26041 aaaagaagtt atagttgtta gaattacact taataaaaaa gttataatta agcagaatat
    26101 tataaaaaca actgtactgt tgatatttct tggagttttc actagctgtc ctccattcta
    26161 tatcctatgc ctcttaatgt ttttatacaa ggtggatttt ttaatttttc tctaagacgc
    26221 tttattgtaa cagtaagagt gttatcattt acgaaattac catctacatc ccagagcatt
    26281 tctagtaact ttgtacgggt catagctcta tttttatttt ccataagact tagaagaagt
    26341 ttatattctc ctgctgtcaa aagtacttca acattatcta aatatgcttt gacttcaact
    26401 ttattcaagc ttatatttcc acatgtaata gtattgttag tatttgaatg agttcttcta
    26461 agaactgcat taattctgga ttgaagtact gagagcagaa atggtttaac cacatagtca
    26521 tctgcaccca tatctaatcc acgtataatg tctttttcat catcacggac tgtcaaaaat
    26581 atcactggag tatcttctat acttttaaca tattggcaaa actcgtatcc aataccatca
    26641 ggtagattta aatcaagcag aattaaatca atagttttat caaaattttt tttagcttca
    26701 gcaatcgtat tataacaaca tacagtgtga tttttctttt caagggaagt ttttattcca
    26761 aatgtaattg tatcatcatc ttctaaaaga agtattttag ccatttactt ttagtccttt
    26821 cagtaattta gttatttcat taactaagtt attagtttta tgtttaatat tttttatgtt
    26881 agagatagaa gttagagata gaaaaaatta tcatttaagt atataaactt taatttataa
    26941 acttaggttt atgctttaat tgaatctatt aaaagagcaa gagttgtttc aataaatttt
    27001 tcctcatcta tattaaagtt gttcaggtat gactttcctg ttacagatag taaattaaaa
    27061 aatcctgtaa gtgtaaatat tgatgaatac atgagagtag gtatttcaat atcatgccta
    27121 atacttctat cattttttcc aatttcaaac attccttgta tttcatcaaa cataaattta
    27181 ttaaaagaaa aaaacttttc tttaaacagc atatcagtat ccgatttttc agagtttaca
    27241 ataccaatca ttcccataag gcataataga gaaggttcta cagagtaaaa tttatgaaaa
    27301 gcaaagtatg aaagttttat tttttcaaac cctgttttgc cttttgcact ttctatttta
    27361 acattatccc aaagtctttt atatcctctt aagacaacag caaagaataa atcttcttta
    27421 catgaaaagt acttgtatat ggtgcgttta gtatattcaa cttcttttgc caactcattc
    27481 atagttgtat tctgaacctc ccatgactaa agtcacaggg ttcctgcttc atagaatttt
    27541 gcatactaaa atatgtctta ctaattctcc acaggccatc cccgtagttc ctacggttct
    27601 tacataagtt atttattttg aatcgttatt agtcttaatc cttcttttaa tatatttata
    27661 cttgcattta tatctctatc gtgagtttca ttacaactag gacaaatcca ttctcttata
    27721 tttaagttct taacttcctc gtttttatac ccacatttat tgcatatttg tgaacttgca
    27781 aagaatttac ctactttata caatttgtct accataccaa ttagccttgt attctaattg
    27841 acgaataaat tctgaccatg atacatcaga aattaagcga gatagtttat gatttttaat
    27901 catattcttt acttgtaaat cttctatgca aataatatca ttctctttta ttaatttagt
    27961 agata
//
LOCUS       DAMJWP010000024        27926 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.025, whole genome shotgun sequence.
ACCESSION   DAMJWP010000024 DAMJWP010000000
VERSION     DAMJWP010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27926
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.025"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(147..1526)
                     /locus_tag="PC700_000959"
     CDS             complement(147..1526)
                     /locus_tag="PC700_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HDF2580279.1"
                     /translation="MINIILEESSLPMLIPEKLAKKYNVFPVEFQDDNLVVEIEDEDI
                     YALQDLRLATGKEIILKKEKKELISEKIEKYYNSFELDEDYAKKLFENLLEKAIKENA
                     SDIHIEPFKEHLIVRMRVDGELKEVSKFLVDVYPSLSTVIKLKASMDITEKRLPQDGR
                     VDIKLGGNLIDIRVSSIPTVYGEKIVLRILNRDTFLKDKLELGFSEEAIKSINRIINK
                     RAGVLLVTGPTGSGKTTTVYSLLNDLKGINKNIMTIENPVEYKMDGISQIQVNSKVGL
                     SFDVGLRAILRQDPDIIMVGEIRDAETAKIAVRAAITGHLVISTLHTNDAVSSIARLL
                     EMQIPPYLLNASLIGVISQKLVRKVCNHCSHEIMIKDNFSGDVNTKVAVGCEKCNDKG
                     YFGRTAIYEILEINDDIKTCIRNMEDSSTIKDVAQNNGMITFEDSCKRLINEKITTLE
                     ECLVVNEMI"
     gene            complement(1519..2199)
                     /locus_tag="PC700_000960"
     CDS             complement(1519..2199)
                     /locus_tag="PC700_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HDF2580280.1"
                     /translation="MKKQYVFIILASTILGILVGTIFRDYSNEETVYTSKDKSIRKEI
                     KMTRKSIKELTKEKDKLDKEINSMKQNQKNSEEISEIETLRDSLSYSDVKGSGIILSI
                     DAIDEKTGNIANSIDYNKILVKVINELKVNGGKYIEINGQRINQYSEVVLAGSHININ
                     GVPVAQPYEIKCIGDKEKLSNYVDKGSKYLDSIQTNYPMKISIKTEENISMKKMNIPN
                     KLKYIKGD"
     gene            complement(2591..3670)
                     /locus_tag="PC700_000961"
     CDS             complement(2591..3670)
                     /locus_tag="PC700_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2580281.1"
                     /translation="MEGKVKKAFPGGNTSQGSYSFFDYIIPENVKRVFCLKGGPGVGK
                     SSLMKKIYKEFLHRGYDIDLYHCPSDPSSLDGLVIKKLGVVLMDATAPHTMDPKLPGA
                     LDEIINLGDYWNTEELEKNKQEISVYDKDISNSFKRAFKFLKSAEPIFRDIEEKYSDC
                     MDYGKVNLVTEEFIKRLFDGVKSTGNLKREKHLFGSAITPVGCLDYTENILKDVKKVY
                     YLDGEIGTGKTTLLNKVYKKATEKGLNVEVYHYPLIPEKIETVLLTDLDIGITISTTF
                     KEEDTIDLNQFLNREKLLKYEEDIKFDEKVLDDLICWAVLNLKRAKSKHDIIESYYSP
                     NMRFDEVGKLRDKLIKRILKYEENL"
     regulatory      3958..4073
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            4248..5618
                     /locus_tag="PC700_000962"
     CDS             4248..5618
                     /locus_tag="PC700_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2580282.1"
                     /translation="MKQKWIVLIIICIGVFMSTLDGSILNIANPTIAADFKINMSQIQ
                     WVVTAYMLVVTATMLFFGKLGDKVGSNRLYALGFFIFTIGSFLCSMSNNLSTLISSRI
                     FQAVGASILMATGLGIVSNAFPANEKGKAIGITGAVVGIGNMSGPVIGGIILEHFGWP
                     SIFIINIPIGIIAVFLGIKFLPKPVLDEQNKSFDISGLLLFASCTTLILLAMNEKGNT
                     RLYLGITALIIFLLLALREVKFEQSFIDLPLFKNRNFTVGNIIGVACYFPQMAVSFLL
                     PFYLEQLKNLSPMMAGYVMTVHPLIMVLIAPIAGSLSDKHGAKNILTASFSFMTISLV
                     GMALLKADSPLYLLIVCLVIFGLGLGAFSSPNNSSILADVPPQKQGYGGSFLATIRNL
                     SFALGTAFFSSFFAQSLTYNQKFKSHTSAYVIASNQSYWIAASVCFIGLILTVFFMRK
                     TDKSIS"
     gene            complement(5883..6494)
                     /locus_tag="PC700_000963"
     CDS             complement(5883..6494)
                     /locus_tag="PC700_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDF2580283.1"
                     /translation="MKIRSSEITMSAVNKSQYPAEGIPEIALAGRSNVGKSSIINTLL
                     NRRNFARTSQTPGKTRTINFYLINNEFYFVDLPGYGYAKIAKSEKEKWGGIMERYLES
                     RQELCSIFLLVDIRHEPTADDKLMYEWIKHFGYNCVVIATKADKISRGQYQKHISIIR
                     KKLQMESSEKVIPVSSLKKTGVEELWEEIVNQYNQHGYEITVD"
     gene            complement(6484..8847)
                     /gene="lon"
                     /locus_tag="PC700_000964"
     CDS             complement(6484..8847)
                     /gene="lon"
                     /locus_tag="PC700_000964"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HDF2580284.1"
                     /translation="MEQNYTKIDHELPLIPLRGLAIFPYMILNFDIGREISLKALDQA
                     MMDEELIFLTSQKEAEVDEPGEEDFYHVGTICKVKQMIKLPGDTVRVLVEGVSRGRVK
                     KIEQEDGYFRAVIEEIVFNSDNLDSETEVEIEAFVRNVFDAFEEYINIGNRVSPEILI
                     SLADIEDVDRFIDTIAANIYLKSSQKQEILEEFDIRKRLELIYSILLEEIDILKIEKK
                     ITLRVKKQMNKVQKEYYLREQLKAIQKELGEEEDINSEADEYREKLKKIKAPKTTKEK
                     IEKEIDKFSKISSMSPDVSVSRNYLDTIFSLPWNKETKDKLDITKAKDILDEDHYGLE
                     KVKERILEYLAIRTLAKSLKGPIICLVGPPGTGKTSIVKSIARALNRKFVRISLGGVR
                     DEAEIRGHRRTYVGSIPGRIINGVKEAQTKNPVFLFDEIDKMAADYKGDPASAMLEVL
                     DPEQNKDFVDHYLEIPFDLSKILFVTTANSLGNIPRPLLDRMEVIEVSGYIEEEKLNI
                     ARKYLLPKQIKEHALKENFIKIDDETLRSIINHYTREAGVRTLERTIGKICRKVAKKY
                     VEDPTLEEVVINKSDLETYLGKDMFKYQLAEVNPQIGLVNGLAWTAVGGVTLEVEVNV
                     LKGKGEIVLTGKLGDVMKESAKTGISYIRSIVDKFDIDPEFYKTNDIHIHIPEGAVPK
                     DGPSAGITMALAVISALTKRPVPGNIAMTGEITLRGRVLAVGGVKEKLLAAHRAGITK
                     VLIPKECEADLDEIPENVKEKMEFVLVEHMDEVLEQALLKSGENNEN"
     gene            9027..9539
                     /locus_tag="PC700_000965"
     CDS             9027..9539
                     /locus_tag="PC700_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HDF2580285.1"
                     /translation="MLKLFLTFLKIGAFTFGGGYAMVPLIQDELVNKQKLLEQEEFID
                     YLSIAQSYPGVLAVNISVLLGYKLYGIPGVLICTLGSALPSFCIVLVLSYLYFKNSSS
                     KILDGFFKGVAPVVIALILYSFTSMFKKLPKSKTNILLLIIAVLSVGVFNISPIYLII
                     GGGVYSLCQK"
     gene            9527..10054
                     /locus_tag="PC700_000966"
     CDS             9527..10054
                     /locus_tag="PC700_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HDF2580286.1"
                     /translation="MSEIILLFLIFLKIGAFSFGGGYAMLPFIQQEFISKYHFITNQE
                     FLDLLAISQSTPGPVAINSATFVGFKTAGFFGSLAATIGVTIFALIGLTIISKLFSKF
                     KNNKKVENLLTVLRPITLGFILSAAFSSAKEVNWDLYGIIAFIASFYLLYKGKIGSIA
                     IVFVFGILGILLTFL"
     gene            complement(10125..11375)
                     /gene="clpX"
                     /locus_tag="PC700_000967"
     CDS             complement(10125..11375)
                     /gene="clpX"
                     /locus_tag="PC700_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HDF2580287.1"
                     /translation="MSKYEEKRQLKCSFCGKNQDQVRRLIAGPNVYICDECVELCDEI
                     IQEEIEDTIDEDTTSLPKPKEMMEILNDYVIGQEKAKKALSVAVYNHYKRIYSKKSSS
                     KDIEIQKSNILLLGPTGSGKTLLAQTLARTLNVPFAMADATSLTEAGYVGEDVENILL
                     KLIQAADFDIEKAERGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPHQEFLKIDTTNVLFILGGAFDGLEKIIQKRGGDKTLGFGAKIESKKEL
                     DLGKLYEKVLPEDLLKYGIIPEFIGRIPVLATLELLDEDALMQILQEPKNALVKQYKK
                     LLELDDVELEFEEGALRAIAKKAIERNTGARGLRSIVESVMMETMFEVPSRDNIKKVI
                     VTEKSVNEDSVNPIIVLKDQEESA"
     gene            complement(11394..11978)
                     /gene="clpP"
                     /locus_tag="PC700_000968"
     CDS             complement(11394..11978)
                     /gene="clpP"
                     /locus_tag="PC700_000968"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDF2580288.1"
                     /translation="MALVPVVVEQTGRGERSYDIFSRLLKDRIIFLGDQVNDATAGLI
                     VAQLLFLEAEDPDKDIHLYINSPGGSITSGMAIYDTMQYIKPDVSTICIGMAASMGAF
                     LLAAGAKGKRLALPNSEIMIHQPLGGAQGQATDIEIHAKRILKIKETLNEILSERTGQ
                     PLEKIKMDTERDNFMSALEAKEYGLIDEVFTKRP"
     gene            complement(12127..13413)
                     /locus_tag="PC700_000969"
     CDS             complement(12127..13413)
                     /locus_tag="PC700_000969"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDF2580289.1"
                     /translation="MKAELIKKEGNKVTLKITVDNNKFEAAVNKAYNKSRNKYNIPGF
                     RKGKAPRIVIETQYGKGIFYNDAIEILFPEVYPEAIKELDIDPIDNPDLDIEEISKDN
                     GLVMVLNVEVKPEFELGNYKGIEIAKVENTVSDENVDAKLQEMVEKNARLVSVEDKAL
                     EDGDTAIIDFEGFENGVAFDGGKGENYNLVIGSNTFIPGFEEQLVGKKAGEEVEVNVT
                     FPEEYHSQDLAGKPVVFNVKINDVKVKELSALDDEFAKDTSEFDSLDELKADVRAKLE
                     EEAKNRADAETRNSVVEKVAENTEIEIPEVMIQHQIDNMLNELNYQLQYQGFGLQQLL
                     EMTGRTMEELREERKEDAKKLVKSSLVLEAITKAEGIEATEEEFKAELEKMASAYNME
                     VEKIEASLRDADKEDIKGQIKIRKTIDLLVDNATIA"
     gene            complement(13607..14077)
                     /locus_tag="PC700_000970"
     CDS             complement(13607..14077)
                     /locus_tag="PC700_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2580290.1"
                     /translation="MKIGIVSDTHMMIKNMEKTIPYLKECDLIIHAGDNFTDSRYIHS
                     MTNVGIIAVKGNCDFDAVEEEVVFEVANKTIFLCHGDKYGVKYGTNMLEKKATEVDAD
                     IVIFGHTHTPFREIKDGVLYINPGSTSLPRGVSYKSFVIMDIEEDDIKIEEIRI"
     gene            complement(14077..15426)
                     /gene="rph"
                     /locus_tag="PC700_000971"
     CDS             complement(14077..15426)
                     /gene="rph"
                     /locus_tag="PC700_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDF2580291.1"
                     /translation="MTRSYDRSNEQIRPVKITRDYTKYAEGSVLIEMGETKVICTASV
                     EEKVPPFLRNSGTGWINAEYSMLPRATQQRKIRDASRGKIDGRTQEIQRLIGRAIRSV
                     VDLNKLGERTIWVDCDVIQADGGTRTASITGAFVAVAEAIYKLYKAKTIKEMPITNFV
                     SAISVGIVNGIHMLDLCYEEDSKAQVDMNIIMTDKCEFVEVQGTGEEKPFSRADLNLL
                     LELGEKGNKELIRLQREALGEIADEILGMDYGNEVVIATNNAHKLEEIGEILKDFEYK
                     VYSLKDVDLAGIEIVEDGKTFEHNALIKARAIAKKTKLIAISDDSGLEVDALGKKPGV
                     YSARYAGEHATDEENRKKLLKAMQNVPMSKRNARFVSAIAVVFPDGKEFVVRGICEGM
                     IGFEEKGKNGFGYDSLFIVKGYDKTFGEIPSVIKNSISHRANALKLMKQEFIKRVVK"
     gene            complement(15586..17610)
                     /gene="ligA"
                     /locus_tag="PC700_000972"
     CDS             complement(15586..17610)
                     /gene="ligA"
                     /locus_tag="PC700_000972"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDF2580292.1"
                     /translation="MSVKDEINKLIETINYHNERYYNQDSPEISDYEYDKLMKELISL
                     EEEHPELKRIDSPTNRVGGKPLDKFNQIVHKTPMLSLSNAFSEQDLRDFDKRVQEYVG
                     ENVEYVVEFKIDGLSVGLTYNNGEFEKGATRGDGVVGEDISQNLMTVKSIPLKINDKK
                     ELIVRGEVYISKDNFRKVNEQQEEQEQPLFANPRNLAAGSLRQLDPKLTAKRPLDIFV
                     FNMENIEEYDLESHSESLEYLEKLGFSVSQSYKVCKSIDEVIEHIEYWTDNRGNLDYE
                     IDGMVIKVNNIKQRDEMGYTAKSPRWAIAYKFPAEKKKTKIVDIIVEVGRTGTITPSA
                     ILEPVRLAGTTVSRATLHNEDYIREKDIKINDTVLVQKAGDIIPQVLEVIKEERTGDE
                     IDFKMPEKCPVCSEPTIRLEGEAAVKCINMSCPAQIRRGIIHFVSRDAMNIDGLGESI
                     ITLLLNEKIIQDVSDLYYIKKEDVVDLERMGEKSADNLINSIEKSKENDLWRLINGLG
                     IKFIGVKAAKILAYNFKDLDEVISATSEQLEELEEFGSIMANSVVEFFKEDKNMNVIN
                     KLKNVGVNTKSLDNTGEKVEKIFEGMKIVLTGTLPTLKRNDAKDMIEKRGGKATSSVS
                     KSTTFVLAGEEAGSKLTKANDLGIKVIDEERFLELLKLSSKDEVKAVLES"
     gene            complement(17796..18734)
                     /locus_tag="PC700_000973"
     CDS             complement(17796..18734)
                     /locus_tag="PC700_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDF2580293.1"
                     /translation="MKVLFTINYGKEKFDKIRELGYDVIHYSEDSIENNEDVNTADVL
                     VTYNPFERLDISKMKNLQYIQTTSVGIDQLPKEEVLKRNIKIANNRGGYSVPIGESIV
                     LYILEIYKNSAKFFKQQQNKEWKMDFSVSELYGQRVGFLGTGTISSEAAKRLKAFGVE
                     IWGVNTDGSSKKYFDKCFATEDMNIVFEECDIVVVAMPSTKSTDGIVNKDMFKLMKDG
                     SVFINVGRGNTVNQKDLEECVEKFRGVALDVFESEPLNKDNKLWECENVIVTPHNSWV
                     SDKNKERTFNMVYNNLKHYIMENKPVDNVVDISRGY"
     gene            complement(18836..19258)
                     /locus_tag="PC700_000974"
     CDS             complement(18836..19258)
                     /locus_tag="PC700_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HDF2580294.1"
                     /translation="MSFEKINNNNLDKIHNRKCIMLVNFNKKELSLIKNVSNFAGIRD
                     HIILSEKNGENIIRDILDGNISSECENGIKSKSVIFNNTPSTQVNAFLEGLKKVRINR
                     PFTAMVTETSIDWTLNNLIENLVQERNAMSLGKTIEHK"
     gene            19446..20729
                     /locus_tag="PC700_000975"
     CDS             19446..20729
                     /locus_tag="PC700_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugated bile salt MFS transporter"
                     /protein_id="HDF2580295.1"
                     /translation="MNTLLNNKFSIKNFSNAWVIVLGCMLIQAVPFSIVASIQPQFIS
                     YVVEGEGFSLASFSLIFTIGTIASAIASPFIGVLFSKVNIKILYLLGCIISSIAFATF
                     SICTELWQFYTVSCIVQIGATIISAIGIPVLISAWFDEESKGKALGFAFAGGSIGSIF
                     LQPLAVRLLATQGYSHSYLIFGIIALIVGIPVILFILRMPKDINEKVKSKGKKEKASD
                     TISPSVWGYTLKEAKKIKYFWIMTIGFIFVGIYVSSLSVQYPTYLRNYLNPILIGTVG
                     STFALFALIGNLFGGLLFDKFGQTKPLIISFVLVLIATLSLLLVDKSSTFAFVFAILK
                     GLSVFAYMIGPAYLTGSFFGQKEFGSIYGIVQLIFAVGFALGSTLFGLLVDNFGYRFS
                     WSIIILSVIIAYTLLIITSIGMKKLNKERLDKLMQ"
     gene            complement(20807..21322)
                     /locus_tag="PC700_000976"
     CDS             complement(20807..21322)
                     /locus_tag="PC700_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2580296.1"
                     /translation="MNYFVIADPDKCIGCRTCMIGCVVAHSDEDIFYQNPDEINFNPK
                     LSVIKTKEVSAPIQCRHCEDAPCAKACPNGGIVRVGNTIKINEENCIGCKTCMLACPI
                     GAIDIVTLKDVDEGKLCFRERMTANKCDFCIESPEGPACVNVCPTKAFTIVKDEDIDS
                     KVKNKRKLAIL"
     gene            complement(21322..22698)
                     /locus_tag="PC700_000977"
     CDS             complement(21322..22698)
                     /locus_tag="PC700_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HDF2580297.1"
                     /translation="MIINIDKDLCTGCRECSKVCPVNAIEGEEGKPQEINLDRCVMCG
                     QCVQTCKSYASVIDEGFEFLQEKKQEREIPESINEPIFAAYNVCDIDKVKKALSDPNV
                     FTMVQCAPAVRVALGEDFGYYLGYLGAGKMAAALRALNFDRVYDTNFAADLTIMEEGS
                     ELIKRVTEGGTLPMFTSCCPAWVKFMEKNYPKLTNHLSSCKSPQQMGGAIFKTYGAEI
                     NNVDASKIFSVSIMPCTCKKYECDREEMDSSGYRDVDVVLTTRELAYLIKDMGIDFKN
                     LREEKFDSPLGSYSGAGTIFGVTGGVMEAAIRTGYELITGESIPDVEVKQVRGENGFR
                     KSTIKIGDLDLRVGVVSGLKNVIPVLEDLEKGKLDVDFIEVMTCPVGCVSGGGQPKIL
                     LEEYKELAYENRTKATYVHDEHLAIRKSHENPDIKKLYDEYLVEPLGEKSHHLLHTTY
                     CIGKEVAK"
     gene            complement(22723..23274)
                     /locus_tag="PC700_000978"
     CDS             complement(22723..23274)
                     /locus_tag="PC700_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2580298.1"
                     /translation="MKNKIGSFVIADPNKCTGCRACEVACFTMHNQHNNVGYTVGTVD
                     IPVIPRLYLVKGDNFCMPIQCRHCEDAPCLNSCPQKAIVKENNIMSVNEEKCIGCKTC
                     LLACPFGAIDLLPQYEDGKEVEQVILDENKKIAYKCDLCKDNEKIACISACPQEALKL
                     VTPIDDKKAKNRQAALSLLLTNK"
     gene            complement(23332..23547)
                     /locus_tag="PC700_000979"
     CDS             complement(23332..23547)
                     /locus_tag="PC700_000979"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580299.1"
                     /translation="MQIHRELSKSENKYMCTKKEEQKCSKCEICGVLIDDTDKVYNNT
                     YNLCKKCKRNISTEKIVKYINIYDESK"
     gene            complement(23516..24349)
                     /gene="fdhD"
                     /locus_tag="PC700_000980"
     CDS             complement(23516..24349)
                     /gene="fdhD"
                     /locus_tag="PC700_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HDF2580300.1"
                     /translation="MNNIYSKTKLQENSKDKVFENSYSLVDYKYLNSFNVEVSKINEH
                     EVSIDNEEIIAEYPLSFILNDKYVNTFLCTPYNLEELIVGFLKTKGYIENKKSLLKFE
                     INESERFAKVTIQEKQKNQDEQIIFLNSLDYITCASVKSNIKINYDTIYSIMDRNLNS
                     SELFKNTGGVHSVSVFNFDKKIVTCEDVARHNAMDKAIGFCVLNDITLKDKIVAVSGR
                     ISLEMILKAAMTQIPIVVSKSAPTNLSIELSKKLNITLIGFVRGRRMNIYANPQRVIK
                     K"
     gene            complement(24398..26542)
                     /gene="fdhF"
                     /locus_tag="PC700_000981"
     CDS             complement(24398..26542)
                     /gene="fdhF"
                     /locus_tag="PC700_000981"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(26126..26128),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HDF2580301.1"
                     /translation="MEKKILTVCPYCGAGCQLYLVVKDNKILRAEPANGRTNQESLCL
                     KGRYGWDFLNDPKILTPRLKKPMIRKQGVLEEVEWDEAINYTASRLNEIKEKYGPDSI
                     MGTGSARGPGNEANYIMQKFMRATIGTNNVDHCARVUHAPSVAGLAYSLGSGAMSNSI
                     PEIENADVLFIFGYNGADSHPIVANRIVKAKKNGAKLIVTDPRVTESARIADIHLPIK
                     GGTNMVLVNAFGNVLIEEGLYNKEFVQNHTQGFDEYKEIVKPYTAKYAEKITGIPEEL
                     IRKAMREYAKGKKAMILYGMGVCQFGQAVDVVKGLASIALLTGNFGRESVGIGPVRGQ
                     NNVQGACDMGALPNVYPGYQNVTDDKIREKFEKTWGVKLSPNNGYSLTQVPDLVLKEK
                     KLKAYYIFGEDPVQSDPDASEVREALDELEFVIVQDIFMNKTALHADVILPATSWGEH
                     EGVYTCADRGFQLMRKAIEPQGDVKPDWQIISEISTAMGYPMNYKNTKEIWDELRQLC
                     PSFLGATYEKIETQGCVQWPCKSESMEDKGTMYLYEGQKFSTPNGKGNLFAAEWRPPM
                     EVEDDEYPFSLCTVREVGHYSVRTMTGNCRTLSSLEDEPGRVQINSNDAEKLGIEDDE
                     LVRISSRRGSVITRATVTDRVKEGATYMTYQWWVGACNELTIANLDPISKTPEYKYCA
                     VKLEKLEDQELAEKCVREEYQSLKDKMTATNI"
     gene            complement(26793..27851)
                     /locus_tag="PC700_000982"
     CDS             complement(26793..27851)
                     /locus_tag="PC700_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDF2580302.1"
                     /translation="MDRLKNIRKSLLQYTFLFLLIGITIYLVFKTLDIKMLSNVMVMV
                     DKKFLFIGALAIMCHIMLEGVVMKIIIESTHKVNIRFIGFKLAIMGFYYNLITPFASG
                     SQPVQIYVLKKCKMPLSKASAVVTNKSIIFQIVVTFYCTILVLMNFAMLEKQMKMIMP
                     LVFLGIAINSFVLIMIILVILNPKKIKYFMRITIKHLSKIKFLKFLDNKIESIESFID
                     DYSKAINFFAKNKKVLISTIIVTFIQLSAYFSVSFWIYKAFNLQGYSYIYILTLQAFL
                     YMAISPIPTPGNVGANELAFFTIFKSVFPQPLMGYAVFLYGGFMYYLILIGSGIFTVI
                     THYRMKNRIGRNIVLSEN"
BASE COUNT         8321 a         5106 c         3090 g        11409 t
ORIGIN      
        1 attaattaca attaaataaa cttaatattt tctaatttaa tgcatttaat tttatttcac
       61 ttaaatataa tttatactca aacatcttaa ttaaaataga ttaaataaag acaactgacc
      121 aaacgaagtt taactataca attcagttaa atcatttcat ttactactaa acactcttct
      181 aatgtcgtta ttttttcatt tattagtctt ttacagctat cttcaaatgt tatcattcca
      241 ttattttgtg ccacatcttt aatagtacta gaatcctcca tatttcttat acatgtcttt
      301 atatcatcat taatttctaa gatttcataa attgctgttc ttccaaagta tcctttatca
      361 ttacattttt cacaccctac agctacctta gtatttacat ctccactaaa attatctttt
      421 atcataatct catggctaca atgattacaa acttttctaa ccaacttttg agatataaca
      481 cctataagag aggcatttaa aagatatggt ggtatttgca tttctagtaa tctagctatg
      541 gaagatactg catcattagt atgtaaagta cttataacta aatgacctgt tatagctgct
      601 cttactgcta tcttagctgt ttctgcatct cttatttctc cgaccattat tatatctggg
      661 tcttgtctta atatcgctct aagacctaca tcaaagctca accctacttt tgaatttact
      721 tgaatctgac ttatgccatc catcttatac tctacaggat tttctatagt cataatattt
      781 ttatttattc cttttaaatc atttaataag gaatatactg ttgtagtctt tccactacct
      841 gtaggacctg tcactaataa tactcctgct cttttattta ttatacgatt tatagatttt
      901 atagcttcct ctgaaaaacc aagttctaat ttgtctttta aaaaagtatc tctattaagt
      961 atcctcaaaa ctattttttc accatagaca gttggtattg atgaaactct aatgtcaatt
     1021 aaatttccac ctaactttat atccacccta ccatcctgag gaagcctttt ttctgttata
     1081 tccattgatg cctttaattt tataaccgta gacaatgatg gatagacatc aactaaaaac
     1141 tttgatactt cctttagttc tccatctacc ctcattctca ctattaaatg ttctttaaat
     1201 ggttctatat gtatatcact tgcattttct tttatagctt tttctaaaag attttcaaat
     1261 aatttttttg cataatcctc atcaagttca aagctattat aatatttttc aatcttttca
     1321 cttataagtt cttttttctc cttcttcaat ataatttctt tacctgtagc taatctcaag
     1381 tcctgtagag cataaatatc ctcatcttct atttctacaa ctaaattatc atcttgaaac
     1441 tcaactggaa atacattata tttctttgca agtttttctg gtattaacat tggcaaactg
     1501 ctttcctcta atataatatt aatcaccctt tatatacttt aatttattag gtatattcat
     1561 ttttttcata gagatatttt cttcagtttt tatgcttatt ttcattggat aattggtttg
     1621 tatgctatct aaatatttac tacctttatc tacataatta gataatttct ccttatctcc
     1681 aatgcacttt atttcatatg gttgtgctac aggaactcca tttatattta tatggcttcc
     1741 tgcaagtaca acttctgaat attgattaat tctttgacca tttatttcta tgtattttcc
     1801 tccgtttact tttaattcat ttataacttt tacaagaatc ttattatagt ctatactatt
     1861 agctatattc cctgtttttt catctatagc atctatactt aatattatcc cactaccctt
     1921 tacatctgaa tatgatagac tatctctaag tgtttctatt tctgatattt cttcagaatt
     1981 tttttgattt tgtttcattg aattaatttc tttatctaac ttgtctttct ctttggttaa
     2041 ttcttttata gattttcttg tcatttttat ttcttttctt attgacttgt ctttgcttgt
     2101 ataaactgtt tcttcattgc tataatctct aaatatggtt ccaacaagta tacctaaaat
     2161 agtacttgct agtattataa acacatattg ttttttcata ctgtattgct ccttacaaat
     2221 ttatttttat atgataattt tctttaatta aatattaaac atttacatat gcttattata
     2281 tcaaagttat atattaaaat tcatctttag tatataatat catacattga actcctatca
     2341 ctaatttacc aatatcaagt atgcattttt agatgttctt aaaattatat aaactttcaa
     2401 taaacattgt tgtgatatta taaaaaaaca taaattagga aaggaaataa taattatgaa
     2461 aaacaaataa tggctttgga gattattaat atcatttttt agcaaaaaaa agtaaaatgg
     2521 catagcctca aagctacacc aatttactaa aaattatatt attattaaca ttaattttaa
     2581 acatatacgt ttataagttt tcctcatatt ttaaaattct tttaattaac ttatctctta
     2641 atttccctac ttcatcaaat ctcatattag gactatagta actttctata atatcatgtt
     2701 tagacttggc tctcttaaga tttagtactg cccaacaaat taaatcatct aaaacttttt
     2761 catcaaattt aatatcttct tcatatttaa gtagtttttc tctatttaaa aactgattta
     2821 aatcaattgt atcttcttct ttaaaagtcg tacttatagt tatgcctata tctaaatcag
     2881 ttaatagtac agtttcaatt ttctctggaa taagtggata atgataaact tctacattta
     2941 aacctttttc agtagctttc ttatatactt tgtttaataa tgtagttttc ccagttccta
     3001 tttctccatc taaataatag actttcttta catcttttaa aatgttttct gtataatcta
     3061 aacaccctac aggagttata gctgaaccaa ataaatgctt ttctcttttt agatttcctg
     3121 tgcttttaac tccatcaaat aaccttttta taaattcttc tgtgaccaaa tttactttgc
     3181 cataatccat acaatcagaa tatttttctt ctatgtctct aaaaattggt tctgctgact
     3241 ttaaaaactt aaatgctctt ttaaatgaat tactaatatc tttatcataa actgatattt
     3301 cttgtttatt cttttctaat tcttctgtat tccagtaatc acctaaatta attatctcat
     3361 ccaaagctcc aggtagcttt gggtccatag tatgtggggc agttgcatcc ataagtacca
     3421 cgcctaattt ctttataact aaaccatcca acgaacttgg gtcagaagga caatgatata
     3481 aatcaatatc atatcctcta tgcaaaaact ctttataaat ttttttcata agtgaggatt
     3541 ttcctactcc aggtccaccc tttaaacaaa aaactctttt tacattttca ggaattatat
     3601 agtcaaaaaa agaatatgag ccttgtgatg tatttccacc tgggaatgcc ttctttacct
     3661 ttccttccat attagcgcct cctcataatt tacaatatct cttaattaag atattcatat
     3721 tataagaatt ttattacagc tttctgattt tgacaaattt aaaacttacc atctaatact
     3781 aataacatat taattttatc taattattat agattctcat actgtacctt attctatcta
     3841 taaatacaat ttaagtgtcc atattgaaat atttgtattg taatacagct ggatattact
     3901 taaatccaat tgtttccatt ataattttat gttaaaataa tattacaaaa tacatctgtt
     3961 tttcttcata aacgggtgaa attccctatc ggcggtaaaa gcccgcgagc cttatggcat
     4021 aatttggtca tattccaaag ccgacagtaa aatctggatg gtagaagaaa atagtatatg
     4081 agtaccttta tgtaatttta catgagtaat ctatacaaat ccttcaacta ccgtatttat
     4141 tcatgaaatt agacacattc aaggtaccta atatacaggt gctttttttg ttgtttattt
     4201 tacaattata tcgtacttat aaaatctatt aagattggag tgttatcatg aaacaaaaat
     4261 ggatagtatt gattatcatc tgtattggtg tatttatgtc tactcttgat ggaagtatac
     4321 taaatatcgc aaatcctaca attgctgctg actttaaaat taacatgtca caaattcagt
     4381 gggttgtaac agcttatatg cttgttgtaa cagctactat gttatttttc ggaaaacttg
     4441 gtgataaagt aggttcaaat cgtctatacg cattaggatt ttttatattt acaataggtt
     4501 cttttctgtg tagtatgtca aacaatttat caacattaat ttcttcaaga atctttcagg
     4561 cagttggagc aagtatttta atggctactg ggcttggtat agtttccaat gcatttccag
     4621 caaatgaaaa aggaaaagct ataggaatca ctggagctgt agttggtata ggaaatatga
     4681 gtggtcctgt aattggaggt ataattttag aacattttgg atggccttct atttttatta
     4741 tcaatatacc tattggtatt atagcagtct ttcttggcat taaatttctg cctaaacctg
     4801 ttttggatga acaaaacaag agctttgata tttcaggtct tctacttttc gcatcttgca
     4861 ctacgcttat attacttgca atgaatgaga agggtaatac aagattgtat ttaggtataa
     4921 cagcacttat aatcttctta ttgttggcat taagagaagt aaaatttgaa caaagcttta
     4981 ttgacctacc tctatttaaa aatagaaact ttactgtagg caatatcatt ggagtagctt
     5041 gttattttcc tcaaatggca gtatcttttc tacttccatt ttatttagaa caattaaaaa
     5101 atttatctcc tatgatggct ggttatgtaa tgactgtaca tccacttatt atggtactta
     5161 tagcacctat tgcaggttca ctttctgata aacacggtgc aaaaaacatt ctaacagcat
     5221 ctttttcatt tatgaccatc tcactagttg gaatggcact tttaaaagca gattctccat
     5281 tgtatctttt aattgtatgt ttagttattt ttggacttgg gctaggagca tttagttcac
     5341 caaacaatag tagtatttta gcagatgttc ctcctcaaaa acaggggtac ggaggcagtt
     5401 ttcttgcaac tattcgtaat ttatcatttg ctcttggaac agcatttttt agtagttttt
     5461 ttgcacaaag tcttacatac aatcaaaagt ttaaaagtca cacatcagcc tatgttatag
     5521 ctagcaatca gtcttattgg attgcagcat cagtatgttt tatagggctt atactaactg
     5581 ttttctttat gagaaaaaca gataaatcta tttcttaaat gctatttatt gctgactaca
     5641 taagtcaata attttttagt tgaactaatt tatactaatt ttcccatacc ttactaaaat
     5701 ttattagtat ttagcaaaat tactgatatt tattgtttat aaacaataag gatgccaaaa
     5761 aaagttcatt aaaactattt taaggcatcc tatattttgc tttaatatta atataattaa
     5821 aactaactat tattacattt attattcaaa taaatcttat gtcaatattt aacattttaa
     5881 atttaatcta ctgttatttc atatccatgt tgattgtatt gatttacaat ctcttcccat
     5941 aattcttcaa caccagtctt ttttagtgaa gaaacaggaa ttactttttc actactttcc
     6001 atttgcaatt tttttcttat tatacttatg tgtttttgat attgtcctct agatatttta
     6061 tctgccttag ttgcaataac tacacagtta tatccaaagt gctttatcca ttcatacata
     6121 agcttatcat ctgctgtagg ctcatgtcta atatctacaa gtaggaatat agaacataac
     6181 tcttgtctac tttctagata tctttccatt atacctcccc acttttcttt ttcagactta
     6241 gctatctttg catatccata gcctggaagg tctacaaaat aaaattcatt atttattaga
     6301 taaaaattta tagttctagt ttttcctggt gtttgactgg ttctagcgaa gtttcttctg
     6361 ttaagcaatg tatttattat tgatgatttt ccaacatttg acctaccagc caatgctatc
     6421 tctgggattc cttctgctgg atactgtgat ttattcacag cactcatagt tatctctgaa
     6481 cttctaattt tcattatttt caccactctt taataaagct tgttctaata cttcatccat
     6541 atgttctaca agcacaaact ccattttttc ttttacattt tctggtattt catctaaatc
     6601 agcttcacat tctttaggaa ttaatacttt tgttatacca gctctatgag ctgctaataa
     6661 cttttcttta acccctccta ctgctaagac tctacctctt aaagttatct ctcctgtcat
     6721 tgctatattt ccaggaacag gtcttttagt aagtgctgag attactgcaa gagccatagt
     6781 tataccagct gatggtccat cttttggaac agcgccttct ggaatgtgta tatgaatatc
     6841 attcgtctta taaaattcag ggtctatatc aaacttgtcc actattgacc ttatataaga
     6901 gatacctgtc ttcgcagatt ccttcataac atctcctaat tttccagtta atacaatttc
     6961 tcctttacct ttaagtacgt ttacttctac ttcaagtgta actccaccaa ctgctgtcca
     7021 tgcaagacca tttactaatc ctatttgagg attaacttct gcaagttgat acttaaacat
     7081 gtcttttcca agatatgtct ctaggtcaga cttatttata acaacctctt caagagttgg
     7141 gtcttcaacg tattttttgg caacctttct acaaatctta ccaatagttc tttctagtgt
     7201 tcttacacca gcttctcttg tataatggtt aattatactt cttaaagttt catcatctat
     7261 ttttataaag ttttctttta aagcatgttc tttaatctgt tttggaagta ggtattttct
     7321 tgctatattt aatttttctt cttctatata cccagatact tctattactt ccattctatc
     7381 taataatggt cttggtatat tacctagact atttgctgtt gtaacaaata gtatttttga
     7441 taaatcaaaa ggtatttcta agtaatggtc cacaaagtcc ttattttgct ctgggtctaa
     7501 tacttctaac attgctgaag ctgggtcacc tttataatct gctgccatct tatctatctc
     7561 atcaaataag aatacaggat tttttgtttg ggcttctttt accccattaa ttattcttcc
     7621 tggaattgaa cctacataag ttcttctatg acctcttatt tctgcttcat ctctaactcc
     7681 acccaaagat attcttacaa attttctatt taatgctctg gcgattgatt ttactataga
     7741 tgtctttcct gttcctggag gaccaactag acatattata ggccctttaa gtgacttagc
     7801 aagagttctt atagctagat actctagtat tctctcctta actttttcta atccataatg
     7861 gtcttcatct aatatatctt ttgccttagt tatatctaat ttatcttttg tctctttatt
     7921 ccatggcaat gagaatatag tatctaaata gtttctactt acagatacat ctggagacat
     7981 agaagatatt tttgaaaatt tatctatctc tttttctatt ttttctttgg ttgtcttagg
     8041 tgcttttatt tttttaagct tttctctata ctcatctgct tcagaattta tatcttcttc
     8101 ttcacctaat tctttctgta tagcttttaa ttgttctcta agataatatt ctttttgaac
     8161 cttattcatc tgttttttta ctcttaaagt tattttcttt tctattttta atatatctat
     8221 ttcctctaat aatatagaat aaataagttc aagtcttttt ctaatatcaa attcttctaa
     8281 tatttcttgt ttttggcttg attttagata tatgtttgca gctatcgtat ctataaatct
     8341 atctacatct tctatatcag ctagagatat taatatttct ggtgataccc tatttccaat
     8401 atttatatac tcttcaaagg catcaaatac atttcttaca aatgcttcaa tttcaacttc
     8461 tgtttcacta tccaaattat cactattaaa tactatttct tctattactg ctctgaaata
     8521 cccatcttct tgttctattt tctttactct accccttgat acaccttcaa ccaaaacacg
     8581 aactgtatct cctggtaatt ttatcatttg cttaacttta cagattgttc ccacatgata
     8641 aaaatcctct tctcctggct catctacctc tgcttctttc tgagaagtta aaaatataag
     8701 ttcttcatcc atcatagctt ggtctaaagc tttaagagat atctctctac ctatatcaaa
     8761 atttaatatc atatatggaa atatagctaa tcctcttaga ggaattaatg gtaattcatg
     8821 atctatttta gtataatttt gttccatcaa cattactcct tttctagtct aattgctttg
     8881 cttttaatat tatatatttg aacaagttaa ttttcaacat taatgataaa ttcttagaat
     8941 actatcatac tatattatct ttttattatg atataatatt aatattagta tacttagatg
     9001 ttatttatct tttggaggtt tctattatgt tgaaattgtt tttaaccttt ttaaaaattg
     9061 gtgcgtttac atttggtggt ggatacgcta tggttcctct tatacaagat gaacttgtaa
     9121 acaaacaaaa acttttagag caggaagaat ttattgatta tttatccata gctcaaagtt
     9181 atcctggagt actggcagtt aatatatctg tattattagg atataaactt tatggtatac
     9241 caggagttct tatatgtacc ttaggctctg ctttaccatc attttgtata gtcttagtct
     9301 tatcgtatct atactttaaa aattcatcat ctaaaatttt agatggattt tttaaaggag
     9361 ttgcgcctgt tgtaatagct cttatattgt attcttttac gagcatgttc aagaaactac
     9421 caaagtctaa aactaacata ctactattaa ttatagcagt tttatctgtt ggtgtattca
     9481 atataagccc tatttattta ataattggag gaggtgtata tagtctatgt cagaaataat
     9541 actactcttc ttaatattcc ttaaaatagg ggcatttagt tttggtggcg gttatgccat
     9601 gctaccattt atccagcaag aatttatatc taaatatcac tttataacta atcaagagtt
     9661 tttggattta cttgctatat cacaatctac accaggtcct gtagcaataa atagtgctac
     9721 atttgtagga tttaagactg ctggcttctt tggttctcta gctgcaacta ttggtgttac
     9781 aatttttgca ttaataggtc taactattat atcaaagtta ttttctaaat ttaaaaataa
     9841 taaaaaggta gaaaacttat tgacagttct tagaccaatt actctaggat tcatattatc
     9901 agcagctttt tcatctgcca aagaagttaa ttgggattta tatggaataa ttgcatttat
     9961 agcatcattt tatcttttat ataaaggaaa aattggttcc attgcaattg tattcgtctt
    10021 tggtattcta ggtatattac tcacattttt atgataatga tacctatttt atatttagca
    10081 tattaacata aaaaaaggag cttttaaagc tccttttcat catcttatgc actttcctct
    10141 tgatccttaa gtactatgat aggatttact gagtcttcgt tcacagattt ctctgttact
    10201 ataaccttct ttatattatc tctagatggt acttcaaaca tagtttccat cattacgctt
    10261 tcaactatac ttctaagtcc tctagctcct gtatttcttt ctatagcctt tttagcgatt
    10321 gcacgaagag caccttcttc aaattctagc tcaacatcat ctaactccaa taacttttta
    10381 tactgtttta ctagagcatt tttaggttcc tgtaatatct gcattaatgc atcttcatcc
    10441 aacaactcta aagtagctaa aactggtatt cttcctataa actctggtat tataccatat
    10501 tttaataaat cctctggaag tactttttca tacagcttcc ctaaatctaa ttctttttta
    10561 ctttctattt tagctccaaa acctagtgtt ttatctccac cacgtttttg aattatcttc
    10621 tctaatccat caaaagctcc acctaatata aataatacat tagtagtatc tatctttaaa
    10681 aattcttgat ggggatgttt tcttcctcct tgtggtggaa catttgcaac agtaccttct
    10741 aatattttta gaagtgcttg ttgaacacct tcaccactaa catctctagt tatagatggg
    10801 ttttcagatt ttcttgcaat cttatctatc tcatctatat atattatacc tctttcagct
    10861 ttttctatat caaaatcagc agcttgaata agttttaaaa gtatattttc aacgtcttct
    10921 ccaacatatc cagcttcagt caatgatgta gcatctgcca ttgcaaaagg tacatttaga
    10981 gttcttgcta gagtttgagc aagtaatgtc tttccagaac ctgtaggtcc taataaaagt
    11041 atattactct tttgaatttc tatatcctta ctactagatt ttttactgta tattctctta
    11101 taatgattat aaaccgcaac tgataaagct tttttagctt tttcttgacc tattacataa
    11161 tcatttagaa tctccatcat ttcctttggt tttggtaaag atgtagtatc ttcatctata
    11221 gtatcttcta tttcttcctg aataatttcg tcacaaagtt caacacactc gtcacaaata
    11281 tatacatttg gacctgcaat taatcttctt acttgatctt gatttttacc gcagaatgaa
    11341 cactttaatt gtcgtttttc ttcatattta gacatattaa cacctctctc tatctacggt
    11401 ctctttgtaa atacttcatc tattaaacca tattctttgg cttctagtgc actcatgaag
    11461 ttatcacgtt cagtatccat tttaattttt tctaatggct gaccagttct ttcagataat
    11521 atctcattta aagtttcttt tatttttaaa attctctttg catgaatttc tatatctgtt
    11581 gcttgacctt gagctccacc taatggttga tgtatcatta tttcactatt tggtagtgct
    11641 agtctttttc cttttgctcc tgctgccaac aagaatgctc ccatagaagc agccatacct
    11701 atacatatag tacttacatc tggtttgatg tattgcatag tatcatatat agccattcca
    11761 gaagttatac ttcctcctgg agaatttata tataaatgta tatctttgtc tgggtcttca
    11821 gcttctaaaa ataatagctg tgctactata agccctgctg tggcatcgtt aacttgatct
    11881 cctaagaaga ttattctgtc ttttaatagt ctagaaaaaa tatcataaga tctttctcct
    11941 cttcctgttt gttcaacaac tacaggtact aatgccatgg tttcataccc cctttattct
    12001 atataattta cagtagcttt tttaagctac tgtaaattat tatacgcatt taactaaatt
    12061 atatttgcac atcattatat actaaacatt tcataacgtc aactatatta gttagcgttt
    12121 attttattaa gctattgttg cattatcaac taataaatct attgtttttc ttatttttat
    12181 ttgacctttt atatcttctt tgtcagcatc tcttaaagaa gcttctattt tttctacttc
    12241 catattgtaa gcagaagcca ttttttctaa ctcagcctta aattcttctt ctgttgcttc
    12301 aattccttca gctttagtta ttgcttctaa tactaaagaa gacttaacta attttttagc
    12361 atcttctttt ctctcttctc ttaattcttc catagttcta ccagtcatct ctagtaattg
    12421 ttgtaatcca aatccttgat attgtaattg gtagttaagt tcatttaaca tattatctat
    12481 ttgatgttgt atcataactt ctggtatttc tatttctgta ttttcagcaa ctttttctac
    12541 tactgaattt ctagtttctg catctgctct atttttagct tcttcttcta acttagctct
    12601 tacatctgct tttaattcat ctaaagaatc aaattcacta gtatctttag caaattcatc
    12661 atcaagtgca gataattctt ttacttttac atcatttatt tttacattaa atacaactgg
    12721 ttttccagct aaatcttgtg agtgatattc ttctgggaaa gtaacattaa cttctacttc
    12781 ttcaccagct tttttaccta ctaattgttc ttcaaatcct ggtataaatg tgtttgaacc
    12841 tataactaag ttataatttt cacctttacc accatcaaaa gctacaccat tttcaaaacc
    12901 ttcgaagtct attatagctg tatctccatc ttctaaagct ttgtcttcta cacttactaa
    12961 tctggcattt ttttcaacca tttcttgtag tttagcatca acattttcgt cacttacagt
    13021 attctcaacc ttagctattt ctataccctt gtagtttcct aattcaaact ctggtttaac
    13081 ttctacatta agaaccataa ccaacccatt atctttactt atttcttcta tatctaaatc
    13141 tggattgtct attgggtcta tgtctaattc cttgatagct tctggataaa cttctgggaa
    13201 taatatttct atagcatcat tgtagaatat accttttcca tattgagtct ctataacaat
    13261 tcttggagct ttaccttttc taaatccagg tatattgtat ttatttctgc ttttattata
    13321 agccttattt actgctgctt caaatttatt gttatctact gttattttta aagtaacttt
    13381 attaccctct tttttaatta gttctgcttt catccttcta atcctcctag tgatatttat
    13441 gtaatacccg ataacggtat tataattcat tctatttata attcaaccac ttaattatat
    13501 cacataagtt tttttattta caagtgttcc attaatatcg ttgtacttat ttgataaaat
    13561 ttaatttttt gagcgttttt tgccattttt ttagataaat acacctttat atgcgtattt
    13621 cttcaatttt tatgtcatct tcttctatat ccataatgac aaaactttta tacgatactc
    13681 ctcttggaag tgaagtgctt ccaggattta tgtaaagtac accatcttta atttctctaa
    13741 atggggtatg ggtatggcca aatataacta tatcagcatc aacttcagtt gccttttttt
    13801 ctaacatatt agttccatat tttacaccat acttatctcc atggcataag aatatggttt
    13861 tatttgccac ttcaaagaca acttcttcct caacagcatc aaagtcacaa ttacctttaa
    13921 ctgcaataat acctacatta gtcatgctat gaatatatct tgaatcagta aagttatctc
    13981 ctgcatgaat tattaggtca cattctttca aatatggtat tgttttttcc atatttttaa
    14041 tcatcatatg tgtgtcgctt actatgccta ttttcattac ttcactaccc tttttataaa
    14101 ctcttgtttc ataagtttta gtgcgtttgc tctatgactg attgaatttt ttataacact
    14161 aggtatttca ccaaaagtct tatcatatcc ttttactata aataagctat catatccaaa
    14221 accattttta cctttttctt caaatcctat cattccttca caaattcctc ttactacaaa
    14281 ttcttttcca tcaggaaaaa ctactgctat tgcagagacg aatcttgcat ttcttttgct
    14341 cattggtaca ttttgcatag cttttaacag tttttttcta ttttcttcat ctgttgcatg
    14401 ttctcctgca tatctagctg aataaacacc aggttttttg cctaaagcat ctacttctaa
    14461 gccagaatca tctgatattg ctataagttt agtctttttt gctattgctc tagcttttat
    14521 caacgcatta tgctcaaatg tttttccatc ttctactatc tctatacctg ctaaatcaac
    14581 atcttttaaa gagtacactt tatattcaaa atcttttaag atttcaccta tttcttctag
    14641 cttatgagca ttattagtag caatcactac ttcattacca taatccatac ccaatatctc
    14701 atctgctatt tcacctaaag cttctctttg aagccttatt aattctttat tccctttttc
    14761 gcctaactct aacaataaat ttaaatctgc tcttgaaaaa ggtttctctt ctcctgtccc
    14821 ctgtacttct acaaactcac acttatcagt cattattata ttcatatcta cctgtgcttt
    14881 agaatcttct tcataacata aatctagcat gtgtattcca tttacaatac caacactaat
    14941 tgcagataca aaatttgtta ttggcatctc ttttattgtc tttgctttat ataatttata
    15001 tattgcttct gcaactgcta caaatgcccc tgttatagat gcagttctag ttccaccatc
    15061 tgcctgaatc acatcacaat ctacccaaat agttctttct cctaatttat tcaagtctac
    15121 aacagacctt attgctcttc ctattaacct ctgtatttct tgtgttcttc catcaatttt
    15181 tcccctagat gcatctctta tctttctttg ttgtgttgct cttggtagca tagaatattc
    15241 agcatttatc catccagtac cactatttct taaaaaagga ggaacttttt cttccactga
    15301 tgcagtgcat ataacctttg tctctcccat ttctataaga acagaacctt ctgcatactt
    15361 tgtataatct ctagttatct ttacaggtct tatctgttcg ttacttctat cataacttct
    15421 ggtcattttt tatatccttt ctcatatttt ttatttttta taaatttatt tttataattt
    15481 ttaattttgt caaatattaa ttattgtcat ttttcgtaat cttattagct tactgaacta
    15541 ttctatcatt aattatagta gttttctact aaattaagcg actttttaac tctctaatac
    15601 agcttttact tcatctttcg atgataactt taataattct aaaaatctct cttcatctat
    15661 aactttaatt cctaaatcat ttgctttagt aagtttagaa ccagcttctt cacctgccaa
    15721 aacaaatgtt gtcgatttac taacactaga tgtagctttt ccacctcttt tttctatcat
    15781 atctttcgca tcatttcttt ttaatgtagg aagtgtacca gtcaatacga ttttcatccc
    15841 ttcaaatatt ttttctactt tttcacctgt attatccaat gattttgtat ttactcctac
    15901 attttttaac ttgtttataa cgttcatatt tttgtcttcc ttaaaaaact cgacaacgct
    15961 gtttgccatt atacttccaa attcctctaa ttcttctagt tgctcactag ttgcagaaat
    16021 tacttcatct aaatccttaa agttatatgc aagtatctta gctgctttaa ctcctataaa
    16081 tttaatacct aatccattta taagtctcca taaatcattt tcttttgatt tttcaattga
    16141 atttattaaa ttgtcagcag atttttcgcc cattctctct aggtcgacta catcttcctt
    16201 ttttatataa tataaatcag atacatcttg aataattttt tcatttaata gcagtgtaat
    16261 tatagattct cccaatccat ctatattcat tgcatccctt gacacaaagt gtataattcc
    16321 ccttcttatt tgggctgggc aagacatatt gatacatttt acagcagcct ctccttctaa
    16381 tcttattgtt ggctctgaac aaactggaca cttttctggc attttaaaat ctatttcatc
    16441 tccagtacgt tcctctttta taacttctaa aacttgaggt attatgtccc ctgctttttg
    16501 aactaatact gtatcattta tttttatatc tttttctctt atataatctt cattgtgaag
    16561 tgtagctcta cttacagtag tacctgcaag tctgactggt tcaagaattg ctgaaggtgt
    16621 tattgtacca gttctcccaa cttctactat tatatctact attttagttt tcttcttttc
    16681 tgctgggaat ttatatgcaa ttgcccatct aggacttttg gctgtatatc ccatttcgtc
    16741 tctttgtttt atattgttta cttttattac cataccatct atttcgtagt ctaaattacc
    16801 cctgttatct gtccagtatt caatatgctc aataacttca tctatacttt tacaaacttt
    16861 ataactctga cttactgaaa atcctagttt ttctaagtac tctagtgatt cactgtgact
    16921 ttctaaatca tattcttcta tattttccat attaaataca aatatatcta atggtctctt
    16981 tgctgttagt tttgggtcta attgtctcaa tgaaccagct gcaagattcc ttggattagc
    17041 aaataatggc tgttcttgtt cttcttgttg ttcatttact tttctaaaat tatcttttga
    17101 tatatatact tctcctctaa ctattagttc ttttttatca tttatcttaa gaggtatgct
    17161 ttttactgtc attaagtttt gagaaatatc ttctcctaca actccatctc ctctagttgc
    17221 tcctttttca aactctccat tgttataagt tagacctaca gaaagaccat ctattttaaa
    17281 ttcaacaaca tattcaacat tttctcccac atattcttga actctcttat caaaatctct
    17341 caaatcttgc tctgaaaaag catttgataa actcaacata ggagttttat gtacaatttg
    17401 attgaattta tctaatggct tacctcctac cctatttgtt ggagaatcaa ttctctttag
    17461 ttctggatgt tcttcttcta aagaaattaa ctccttcatt aacttatcgt attcataatc
    17521 tgatatttct gggctatctt gattataata cctttcatta tggtagttta ttgtctctat
    17581 aagtttatta atttcatctt ttacgctcac tttttgcacc tcttcatatt tttaaaatta
    17641 aattattttg aaatttccct ataattaatt atctcctatt tatatatttt ttagtatatc
    17701 acttttttga ctttaaattt attacaaaaa aaactcttat tagtatcttt aatacgacta
    17761 ataagagttt ttatttttat caaaattttt tatttttaat agcctcttga tatgtctaca
    17821 acattatcta caggtttatt ctccattata tagtgtttca aattattata aaccatatta
    17881 aatgttctct ctttgttttt atctgacacc caagaattat gaggagttac tatgacattt
    17941 tcgcactccc acaacttatt gtccttgttt aaaggttcac tttcaaatac gtctagtgct
    18001 acccctctaa atttctctac acattcttct aaatctttct gattaactgt atttcctcta
    18061 cctacattta taaatacaga accatctttc ataagtttaa acatatcttt gtttactatt
    18121 ccatctgtac tttttgtaga aggcattgct acaactacta tatcacattc ttcaaataca
    18181 atattcatat cttcagtagc aaaacactta tcaaagtatt ttttactact tccatcagta
    18241 ttgacacccc atatttctac accaaaagct tttaatcgtt ttgctgcttc tgaagatata
    18301 gtacctgttc ctaaaaatcc aactctttgt ccatacaatt ctgatacaga aaaatccatc
    18361 ttccattctt tattttgttg ttgtttaaaa aattttgcac tatttttata tatttctaaa
    18421 atatacaata caatactttc accaataggt acactatatc cgcctctatt attagctatc
    18481 ttaatatttc tcttcaatac ctcttctttt ggaagttggt ctatacctac actagttgtc
    18541 tgtatatatt gtaaattttt catcttagaa atatctaatc tctcaaatgg attgtatgta
    18601 actagtacat ctgctgtatt tacatcctca ttattttcaa tactatcttc agagtaatgt
    18661 atgacatcat agcctaattc tcttatttta tcaaattttt cttttccata atttattgta
    18721 aataaaactt tcatctcaaa tctcctctat ctacccacat ttaaaatttt atattctaaa
    18781 atataatatg atactcaatg tacattagtt aaaattacta taagatatct taaatttact
    18841 tatgttcaat tgtttttcct aaagacatag cattacgttc ttgaactaaa ttttctatta
    18901 aattattcaa tgtccaatca atagatgtct ctgttaccat agctgtaaaa ggtctattta
    18961 ttctcacttt ttttagacct tctaaaaatg catttacttg tgtgcttgga gtgttattaa
    19021 atattacaga cttactctta atcccatttt cacattcact tgatatattt ccatctaaaa
    19081 tgtctcttat tatattctct ccgttttttt cacttaagat tatatggtct cttattccag
    19141 caaagttact tacgttttta attaatgata attctttttt attaaagttt acaagcataa
    19201 tacacttcct attgtgtatt ttatctaggt tattattatt tattttttca aatgacattt
    19261 tatccatcct ttcatttatt tatatacttt aatttacccc atacatttaa aataatcaat
    19321 ttttatagtt aatttttata ttaataccca aattacattt aaatagttag gctatccaaa
    19381 actaatatat aaaattagaa taattagatt aatatctaca aatcataacc taaaggagag
    19441 ataccatgaa tacattacta aacaataaat ttagcattaa aaacttttca aatgcttggg
    19501 ttatagtact tggttgtatg ttaatacaag ctgttccatt tagtattgta gcaagtatac
    19561 agcctcaatt tataagttat gttgtagaag gagaaggctt tagtttagct tctttttctc
    19621 taatattcac tattggtact attgcctcag caattgcatc accttttata ggtgtattat
    19681 tttctaaagt caatattaag atactatatt tattaggttg tataatttct agtattgctt
    19741 ttgctacttt ttctatatgt actgaactat ggcaatttta cactgtatca tgtattgttc
    19801 aaattggcgc tacaattata tctgctatag gaatacctgt tttaataagt gcttggtttg
    19861 atgaagaaag caaaggaaaa gctttgggct ttgcttttgc tggtggttct attggtagta
    19921 tatttctaca accacttgca gttcgcttat tagcaactca aggatacagt cattcttatc
    19981 taatatttgg aattatagca ttaatagttg gtatacctgt aatattattc atactcagga
    20041 tgcctaaaga tattaatgaa aaggtaaaat ctaaaggtaa aaaagagaaa gcatctgata
    20101 caatttctcc ttcagtatgg ggttatacat taaaagaagc taaaaaaatt aaatattttt
    20161 ggattatgac tattggattt atatttgttg gtatatatgt ttcatcatta tccgtacaat
    20221 atccaactta tcttagaaat tatctaaatc ctattctaat aggaacagta ggctccacat
    20281 ttgctttatt tgctctcatt ggtaatttat ttggaggatt attgtttgat aaattcggtc
    20341 aaacaaagcc acttattata tcttttgtat tggttcttat agcaacttta tcattactat
    20401 tggtagataa atcttctact tttgcatttg tatttgccat tttgaaaggt ctttctgtat
    20461 ttgcttatat gattggtcct gcttatttaa ctggctcttt ctttggtcaa aaggagtttg
    20521 gtagcatata tggaatcgta caattaattt ttgcagtggg atttgcatta ggttcaacat
    20581 tatttggatt gttagttgat aattttggat acagattttc ttggtctatt ataattttat
    20641 cagttataat tgcctatact ttacttatta taacttctat aggaatgaaa aaattgaata
    20701 aagaaagatt agataaacta atgcaataat tgatttttag atatataaat attataataa
    20761 aaaagatgga gcttttaatc ataaataaaa ctccatcttt tttatattac aatatagcta
    20821 attttctctt atttttaact ttactatcta tatcctcatc cttaactata gtaaatgcct
    20881 tagttggaca aacatttaca catgcaggcc cttctggcga ttctatacaa aaatcacact
    20941 tatttgctgt cattctttct ctaaagcaaa gctttccttc atctacatct tttaatgtaa
    21001 ctatgtctat tgcacctata ggacatgcta acatacaagt cttacatcct atacaatttt
    21061 cttcatttat ttttattgtg ttcccaaccc taactatacc accatttgga catgcctttg
    21121 cacaaggagc atcttcacaa tgtctacact gtattggagc acttacttct tttgtcttta
    21181 tgacagaaag ttttggatta aaatttattt catctggatt ttggtaaaaa atatcttcat
    21241 ctgaatgtgc aactacacat cctatcatac aagttctaca tcctatgcat ttatcagggt
    21301 ctgctataac aaaatagttc atcacttagc cacttccttt cctatacaat atgtagtgtg
    21361 taatagatga tgagattttt ctcctaatgg ttctaccaga tattcatcat acagtttttt
    21421 aatatcagga ttctcatgag attttctaat tgctaaatgt tcatcatgca cgtatgttgc
    21481 ttttgttcta ttttcataag ccaattcctt atattcttct agtaaaattt ttggttgacc
    21541 tccaccactt acacacccaa caggacaagt cataacttca ataaaatcta catctaattt
    21601 tcctttttcc aagtcttcaa gcactggtat tacatttttt aatccactta ctactccaac
    21661 tcttaagtct aagtctccta ttttaatagt tgactttcta aaaccatttt ctcctcttac
    21721 ttgttttact tctacatcag gaatactttc tccagttata agctcatatc ctgttcttat
    21781 agcagcttcc ataaccccac cagtaacacc aaagatagtt ccagctccac tataactacc
    21841 taaaggacta tcaaattttt cttctcttaa atttttaaaa tcaattccca tgtcttttat
    21901 taaataagct agttctctag tagttaatac tacatctaca tctctatatc cactagaatc
    21961 catttcttct ctatcacatt catatttttt acatgtacat ggcattatag aaacactaaa
    22021 tatctttgat gcatctacat tatttatctc tgcaccataa gttttaaata ttgcaccacc
    22081 catctgctga ggtgatttac aagaagataa atgattagtt aatttaggat aatttttttc
    22141 catgaatttt acccaagctg gacaacaaga tgtaaacatt ggaagagttc caccttctgt
    22201 cactctttta attaattcac taccttcttc cattatagtt aaatctgctg caaaattagt
    22261 atcgtagact ctatcaaaat ttaaagctct aagtgctgct gccatttttc cagcacctaa
    22321 gtatcctaaa taatatccga aatcttctcc taaagcaact ctaactgctg gtgcacattg
    22381 gaccatagtg aatacatttg ggtcacttaa agctttcttt accttatcta tatcacatac
    22441 attgtatgct gcaaatatag gttcatttat agactctggt atctctcttt cttgtttttt
    22501 ctcttgtaaa aactcaaatc cttcatctat aactgaggca taagatttac aagtttgaac
    22561 acattgaccg cacataacac atctatctaa atttatctct tgtggctttc cctcttcacc
    22621 ttcaatagca tttactggac agacctttga acattctcta catccagtac ataaatcctt
    22681 atctatattt attatcataa acgtcaccta cccctgtatc ctttatttat ttgttaacaa
    22741 taaactaagt gcagcttgtc tattcttagc ttttttgtca tctattggag taacaagttt
    22801 taatgcttcc tgaggacatg cacttataca agctattttt tcattgtctt tacataagtc
    22861 acatttataa gctatcttct tattttcatc taaaattact tgttctactt ctttaccatc
    22921 ttcatattgt ggaagtaagt ctatagctcc aaaaggacat gctagcagac aagttttaca
    22981 tcctatacac ttttcttcat ttacagacat aatattgttt tctttgacta tagctttttg
    23041 tggacatgag tttaaacaag gagcatcttc acaatgtcta cactgtattg gcatacagaa
    23101 attgtcacct tttactaaat aaagtctagg tataacaggt atatcaactg ttccaactgt
    23161 ataacccaca ttattatgct gattatgcat tgtaaaacaa gctacttcac aagctctaca
    23221 acctgtacat ttgtttgggt ccgctattac aaaagagcct attttatttt tcatattatc
    23281 ccacctttca atttgtatat ttccaaccct ctagttactt tcatcatatg tctacttact
    23341 ttcatcatat atatttatat atttcactat tttctcagta cttatatttc gtttacattt
    23401 tttgcacaaa ttgtatgtgt tattatacac cttatcagta tcgtcaatta atactccaca
    23461 gatttcacac tttgaacatt tttgttcttc ttttttagta cacatatact tattctcact
    23521 ttttgataac tctctgtgga tttgcataaa tattcatccg tcttcctctt acaaatccaa
    23581 taagcgttat atttaatttt ttagaaagct ctattgataa attagtcggt gctgattttg
    23641 atactacaat aggaatttga gtcatagctg cttttaatat catttccaat gatattcttc
    23701 cacttacagc cacaatttta tcttttaatg ttatgtcatt taaaacacaa aatccaatag
    23761 ccttatccat tgcattatgc cttgctacat cttcacaagt gactattttt ttatcaaaat
    23821 taaatacaga tacgctatgt accccaccag tatttttaaa taattctgat gaatttaaat
    23881 ttctatccat tatactgtaa atcgtatcat aatttatttt tatattgctc tttacagaag
    23941 cacatgttat atagtctagt gaatttaaaa atataatttg ctcatcttga tttttttgtt
    24001 tctcttgaat agttactttt gcaaaacgtt cactctcatt tatctcaaat tttaataagc
    24061 tttttttgtt ttctatgtat ccttttgttt tcaagaatcc tactataagt tcctctaaat
    24121 tatatggggt acataaaaat gtatttacat atttatcatt taaaataaaa ctaagtggat
    24181 attctgcaat tatttcttca ttatctattg atacttcatg ttcatttatt ttgcttactt
    24241 ctacattaaa agaatttaaa tatttgtagt ccactagact ataactattt tcaaaaactt
    24301 tatccttact attttcttgt aatttagttt ttgagtatat attgttcata aaatattagc
    24361 ctttctgcct tttataaatc ttaaatctaa taaatcatta gatatttgta gctgtcattt
    24421 tgtctttcaa actttgatac tcttctctta cacatttttc tgctaattct tggtcttcaa
    24481 gtttttcaag tttaacagca caatatttat actctggtgt tttagatata gggtctaaat
    24541 ttgctattgt aagttcattg caagctccaa cccaccactg ataagtcata tatgttgcac
    24601 cttccttaac tctatcagta actgttgctc ttgttattac acttcctctt cttgaactaa
    24661 ttctaactaa ttcatcatct tcaattccta atttttcagc atcattagaa tttatttgaa
    24721 cacgtcctgg ttcatcttct aatgaactaa gtgttctaca attaccagtc attgttctta
    24781 ctgaatagtg tccaacttct cttacagtac ataaactaaa tgggtattca tcatcttcaa
    24841 cttccatagg gggtctccat tcagcagcga acaaattacc tttaccatta ggagtagaaa
    24901 acttttgacc ttcatacaga tacatagttc ctttatcttc catactttca ctcttacaag
    24961 gccattgaac gcatccttga gtttctatct tttcataagt agcacctaag aaacttggac
    25021 ataattgtct aagttcgtcc catatttcct tagtattttt ataattcata ggatatccca
    25081 ttgctgtaga aatttcgctt ataatttgcc agtctggttt tacatctcct tgtggctcta
    25141 tagcttttct catcaattgg aatcctctat ctgcacatgt atatactccc tcatgttctc
    25201 cccaagaagt agctggaagt ataacatcag cgtgaagtgc tgtcttattc ataaatatat
    25261 cttgaacaat gacaaattcc aattcatcta atgcttctct aacttctgat gcatctgggt
    25321 cactttgcac tgggtcttct ccaaatatat aataagcttt aagctttttc tcttttagta
    25381 ctaagtctgg aacttgagtt aaactataac cattattagg agataatttt accccccaag
    25441 ttttttcaaa cttttctctt atcttatcat cagttacatt ttgatagcca ggatatacat
    25501 taggaagagc tcccatatca caagctcctt gaacattatt ttgtccacgt acaggtccta
    25561 ttccaacgct ttctctacca aaattacctg taagtagagc aattgatgca agtcccttaa
    25621 ctacatcaac agcttgacca aattgacaga cacccattcc ataaagtatc atagcttttt
    25681 tcccttttgc atattctctc atagcttttc ttataagctc ttctgggatt cctgtaatct
    25741 tttcagcata tttagctgta tatggtttaa caatttcttt atactcatca aatccttgtg
    25801 tatgattttg aacaaattct ttattgtaaa gaccttcttc tattaataca tttccaaaag
    25861 catttactaa aaccatgttt gttccacctt ttataggtag atgtatatca gcaattcttg
    25921 cagattcagt tactctaggg tcagtaacaa ttaatttagc accatttttt ttagctttta
    25981 ctattctatt tgccactatt ggatgtgaat ctgctccatt atatccaaat ataaataaaa
    26041 catctgcatt ttctatctct ggtatagaat tagacattgc accactacct aatgagtaag
    26101 ccagaccggc tacagatggt gcatgtcata cacgagcaca atggtcaaca ttattagttc
    26161 ctattgtagc tctcataaat ttttgcatta tatagtttgc ttcattacca ggtcctcttg
    26221 cagaacctgt tcccataata gaatcaggtc catatttttc tttaatttca tttagtcttg
    26281 aagctgtata gtttatagcc tcatcccatt caacctcttc tagtacacct tgttttctta
    26341 tcattggctt ttttagccta ggtgttagaa ttttagggtc atttagaaaa tcccatccat
    26401 aacgaccttt taaacacaaa ctttcttgat ttgttctacc attagccggt tcagctctta
    26461 atatcttatt atcttttact actagatata attggcatcc agctccacag tatggacaaa
    26521 ctgttaaaat ttttttctcc atacaattcc ccccttagat ttatcccctg ataagtaaaa
    26581 tttatcttaa agataatgag tgtttttata atatgcaaaa tcataaatat tttgcaatac
    26641 tatctattaa attcaacaat tttctcacaa ttcctcttaa atttcaaaaa aaatatgtca
    26701 taataatctt tatatttcaa ccatttaatt aaaatattct agttcttatg acatattttt
    26761 taatatactc aatatatatt ctttaaactt atttagtttt cacttaaaac aatatttctg
    26821 ccaattctat ttttcattct atagtgagta attacagtaa atattccact tccaatcaga
    26881 attaaatagt acataaatcc accatataaa aatactgcat atcccatcaa aggttgaggg
    26941 aatactgatt tgaatatagt gaaaaaggct aattcattag ctcctacatt cccaggtgtt
    27001 ggtattggag atattgccat atataaaaat gcttgtaatg tcagtatata aatatagcta
    27061 taaccttgta aattaaatgc tttatatatc caaaatgaaa cactaaagta agcacttaat
    27121 tgaataaaag ttactattat agttgaaatt aatacctttt tattctttgc aaaaaaattt
    27181 attgctttac tataatcatc tataaaactt tctatggatt caatcttatt atctaaaaat
    27241 tttaaaaact taatttttga taaatgcttt atagtaattc tcataaaata ttttattttt
    27301 ttagggttta aaataactaa tattatcata attaagacaa atgaattaat ggctatccct
    27361 agaaacacta gaggcataat cattttcatt tgtttttcta acatagcaaa attcattaat
    27421 acaagtattg tacaataaaa agtaaccact atttggaaaa taatagactt attggtaaca
    27481 actgcacttg ccttactaag tggcatttta cattttttaa gtacatatat ctgtactggt
    27541 tggcttcctg aagcaaaagg agttattaaa ttataataaa atcccataat ggctagctta
    27601 aaaccaataa atctaatatt aactttgtga gtgctttcta taattatctt cattactact
    27661 ccttcaagca ttatgtgaca cattattgcc aaagcaccta tgaataaaaa cttcttatct
    27721 accataacca taacatttga tagcatttta atatccaatg ttttaaacac taggtaaatt
    27781 gttataccta ttaacaaaaa taaaaatgta tactgtagta aactctttct aatattcttt
    27841 agtctatcca ttagaaataa ccatcctttg ctcaagattt atactaaata ctatattatt
    27901 agtatacact atgaatctta atattt
//
LOCUS       DAMJWP010000025        27762 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.026, whole genome shotgun sequence.
ACCESSION   DAMJWP010000025 DAMJWP010000000
VERSION     DAMJWP010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27762
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.026"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(74..613)
                     /gene="scpB"
                     /locus_tag="PC700_000983"
     CDS             complement(74..613)
                     /gene="scpB"
                     /locus_tag="PC700_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HDF2580303.1"
                     /translation="MKREDIKYIIESVMFAYGEPISIKELNYIINKELSSKEIEIMLN
                     LLIEEYREQNRGIQIIKLENKYQMCTNKDYAEYIKKIIEPKKKKSLSQATLETLTIIA
                     YKQPITKVEIEDIRGVKCDKVLQTLFENELIREAGRLNKIGKPIIYKTTDEFLKLLNI
                     ESLEELPPIENYQEVATNE"
     gene            complement(615..1334)
                     /locus_tag="PC700_000984"
     CDS             complement(615..1334)
                     /locus_tag="PC700_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HDF2580304.1"
                     /translation="MKYNIQLQVYEGPLDLLYDLITKHKIDIKDISIIDITKQYLNYL
                     KMLDKMDLEITSEFITMASKLLEIKSKYLLYKQKDEEEDPRIELMEKLEEYRKFKVAS
                     QDIKENITYVNERFYRNKEEIIIDDNVDLEDISIEAIKNILPYIFKVKTSQIENANDE
                     KLDKIVRKKIISVEEKILYIRDIIKDKIEVTFTNIIKSYENDEIIATFLSILELIKEK
                     EIVVVQDIFFDDILIRKSSEC"
     gene            complement(1364..1960)
                     /locus_tag="PC700_000985"
     CDS             complement(1364..1960)
                     /locus_tag="PC700_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HDF2580305.1"
                     /translation="MLSMNIGEIVASLVGIAMAISIHEFGHAYSAHLLGDDTAKAYGR
                     MTLNPVRHVDPIGLLAMFILKIGWAKPVPVNPNNFKNYKIGNLIVSLAGVFCNVLTAI
                     LCVIINKYVHMYAINTIAGYVLIYSMGFAAFNLLPIPPLDGWGVISTFVPYKWNEYLY
                     KFESMSYIILLIALFTGIYQIILSPIYTVLLKIVYLFV"
     gene            2258..3094
                     /locus_tag="PC700_000986"
     CDS             2258..3094
                     /locus_tag="PC700_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2580306.1"
                     /translation="MRQEERTVCFDRELKIEAYRFKGIMQKFPNHFHEHYVIGFIEKG
                     QRYLSCKNKEYTTSTGDLLLFNPFDSHTCEQIDDKVLDYRCINIKPEIMKKTVFEITG
                     KNYLPKFNQPVIFRSELVPLLQELHYIIMEEELDFKKEELFFFLIEQLIEEHTEPNLQ
                     SNLENTNIEIQAVCDYLENNYAEHIVLDELSTIAGMNKYSLLRNFTKLKGITPYRYLE
                     NIRVNKAKKLLEKGVEPIDAAIQTGFVDQSHFTNFFKNFIGLTPKQYQNIFINDDSNN
                     FK"
     gene            3115..3537
                     /locus_tag="PC700_000987"
     CDS             3115..3537
                     /locus_tag="PC700_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HDF2580307.1"
                     /translation="MENKNSKCVMVIDENLPMGIISNTAAIMGITLGKHAPETVGPDV
                     IDKTGNSHLGIIDIPVPILKGNKEIIKDLRKKLYTLEFNDLTVVDFSDVAQSCNLYEE
                     FTQKIASVPEDELQYFGIAIYGNKKKVNKLTGSMPLLR"
     gene            complement(3748..4911)
                     /locus_tag="PC700_000988"
     CDS             complement(3748..4911)
                     /locus_tag="PC700_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDF2580308.1"
                     /translation="MKKLASTALAILIALTPLSFSFANNKENADANQLNISSKSAILM
                     DVGSGQILYEKDAHKKLPPASVTKVMTMLLIVEALDSGKIKLDDEVQVSETASSMGGS
                     QIFLEPGETQKVDTLLKGIAVASANDACVAMAEHLAGSVEGFVDRMNAKAKELNMNDT
                     HFANTNGLPVANHYTSAHDIALMSRELLKHEMISKYLTTWMDKVVVGKKQVTVGLANT
                     NKLIKHYQGATGVKTGFTQEAKYCLSASAKRGNTHLVAATLGAETSPERFNDASSLLN
                     YGFANYESVKLCSKGDNIATLTLDKADENKVKLVAKEDLNALIKKGSSKEFEKKIEIV
                     KNPKMPIKKGTVLGKIKICKDKKVIGEVELINTKDINKASYLQMLQRIIDNMI"
     gene            complement(4965..5165)
                     /locus_tag="PC700_000989"
     CDS             complement(4965..5165)
                     /locus_tag="PC700_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protein"
                     /protein_id="HDF2580309.1"
                     /translation="MDDVSRQNAIKALKQTKMEIAGEYGMNYEDAFEIIENASNKGVL
                     EGYFKKLEKKKNLGQGISRHLE"
     gene            complement(5371..7047)
                     /locus_tag="PC700_000990"
     CDS             complement(5371..7047)
                     /locus_tag="PC700_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDF2580310.1"
                     /translation="MQLFKKNTNKIKVMALGGLNEVGKNMTVVEYKDEIIVIDAGLSF
                     PEDEMLGVDIVIPDITYLVKNRDKIKGIFITHGHEDHIGALPYILKKINVPVYGARLS
                     IGLIQVKLKEHKMNNVKLNVIGPRQVIKLDNMEVEFLKNNHSIPDAYSIAIHTDQGII
                     YHTGDFKIDLTPIDGDVMDMHRICELSKKGVLLMLADSTNAEKPGFTMSEKTVGVGLD
                     ELFAKGNGRRIIVATFASNIHRLQQIINTAEKFNRKVAISGRSMVNVVGVAKELGYLD
                     ISDDMLIDLNDICKYEDSELVIITTGSQGEPMSALARMAFSEHKKVEIKSGDLVIISA
                     HPIPGNEKLISRVINFLFEKGAEVVYSDIADIHVSGHACQEELKLIHALVRPKFFMPA
                     HGEYRMLKRHAEIAEQLGMDKENIFVMQTGDVLELDKNSAKVANRIQTGNILVDGLGV
                     GDVGNIVLRDRKHLSEDGLMIVVVTISKDEGKVLAGPDIISRGFVYVRESEDLMDGAK
                     DIIKNVLNECEEKNIKEWAYLKNNIKENLKEYLYQKTKRNPMILPIIMEV"
     gene            7264..8001
                     /locus_tag="PC700_000991"
     CDS             7264..8001
                     /locus_tag="PC700_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDF2580311.1"
                     /translation="MRGKTHCAIGILTAIQTSLIFKIPISLVDILVSATFAVLPDLDK
                     SNSMVSNFILKNNVSKYIYRIFIYAVNIIIFFISININDNFYLSAIITFVAIIIIEAK
                     LTHTFLRKVFLSLIFILLAICLYIIEVEIYFTIFCLMLSIFPWLKHRSFSHSIFATII
                     VYFLLKQIELITNINNLSFYGTIGYASHMFLGDLFTKQGIPIFYPLSEKKISLGFLTV
                     GGPFSNFIEKSFIFVLIGLIIFSILKL"
     gene            complement(8118..8576)
                     /locus_tag="PC700_000992"
     CDS             complement(8118..8576)
                     /locus_tag="PC700_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDF2580312.1"
                     /translation="MANTMDLLKDKLKETGFKITPQRRAIVEILLKHDHSHLSSEEIY
                     DLVRVDCPEIGLATVYRTMQLLDEIGLISKLNLDDGCIRYEISLHKEDCHNHHHLICK
                     NCGKIMEAKEDLLDNIEKEIQSLYKFKILDHDVKFYGLCDECNGVSDSEE"
     gene            complement(8858..9145)
                     /locus_tag="PC700_000993"
     CDS             complement(8858..9145)
                     /locus_tag="PC700_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HDF2580313.1"
                     /translation="MEENIINLIDENGVESQFEIILTLEAEGKEYAILMPLDDEEAEE
                     ALIFRIDEDEEGEILIPLESDEEYETVVAVYTAIMEEEGLNYDEDESNGLN"
     gene            complement(9223..9648)
                     /gene="ruvX"
                     /locus_tag="PC700_000994"
     CDS             complement(9223..9648)
                     /gene="ruvX"
                     /locus_tag="PC700_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDF2580314.1"
                     /translation="MLDGRIMGLDVGDKTIGVAVSDLMGLTAQGVKTIKRVGKKKDIE
                     ELKAIIKEKQVNKIVSGLPKNMNGTLGPQGEKVIKFCELVKAETGIDVEFWDERLSTV
                     AAERSLLEADVSRQKRKKVIDMLAAVIILQGYLDFKINS"
     gene            complement(9854..12025)
                     /locus_tag="PC700_000995"
     CDS             complement(9854..12025)
                     /locus_tag="PC700_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580315.1"
                     /translation="MRKKIMSLFAIVIMIFVSTASYGFAEEKGKVIFIDMNRTNLGSM
                     MNIPILKNEIEKRGYVALMNIRGDQGTDDRRSYASMGAGTRASVTTEDYINFQKVDQD
                     TAKAFEVATGQKAKEINDLGINRSINENLEYGEYGANLGSLGKTLSENGLKTSVIGNA
                     DIIENNQLVKNRNLCLVAMDQYGRIDSGNIDNINKKDSKMPFGISTDYDKLLDETKKS
                     YKNSDAIFVELGDTYRLDMYRLNLNDNTYGTMKKNIVKNINKYLEQVFSMVGENDTVY
                     IASAFPSDLDYKNKRRLSPIIKFSGEGKGLLESATTRKAGIVANLDVGVDILGTFGLE
                     NKEMVGRSYTLVNKEDNVGYLKSLYERIVSISSIRSTIINGFVGVISASWVIAMFAIF
                     FRNYMPHKERVFKVLKEFIKLGIIMPLSFMLAPLMNFKTPISISLGIFATTLILYLLG
                     RKLFKDNDIKHMGFFATLTILVIVIDSVFGTYLMQNNIMSYDAVIGARYYGVGNEYEG
                     VTIASAVFGLAVLLHYKKVSKLLVVIFSLIILITSAYPSMGANVGGAISECVAYLLFI
                     MLIYDIKIDFKKAVLLAVSAVVVVFAFAALDILSGSESHLGMFVQRIFLNGPGEILQT
                     FGRKIQMNMQLAQTSVWVNILLVGIGVIAVLIFKPSRHFRKIMNNFPILFKGFIASMV
                     GCIITLLVNDSGIVAASTASIYILIPLIIININMIIFEKNN"
     gene            complement(12035..12292)
                     /locus_tag="PC700_000996"
     CDS             complement(12035..12292)
                     /locus_tag="PC700_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HDF2580316.1"
                     /translation="MEKDLDYTMKFEGIPEDRMSVGDTIDFVYKALVEKGYNPINQII
                     GYLLSGDSSYITSHKNARAIIKKFERDEILEEVITHYLNRK"
     gene            complement(12317..14956)
                     /gene="alaS"
                     /locus_tag="PC700_000997"
     CDS             complement(12317..14956)
                     /gene="alaS"
                     /locus_tag="PC700_000997"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDF2580317.1"
                     /translation="MEKMGLNEIRSKFLEFFESKGHYVANSYSLVPNNDKSLLLINSG
                     MAPLKNYFSGVEVPPSVRMCTSQKCIRTGDIENVGITARHATFFEMMGNFSFGDYFKR
                     ESIKWGWEFVTEWLNIPEDKIWVTVYEEDDDSYDIWAKEMNFPEERMVRLGKDDNFWE
                     IGTGPCGPCSEIYFDRGEEYGCDNPDCKPGCDCDRYLEFWNHVFTQFDRDEEGNYSLL
                     ENKNIDTGMGLERMGCIMQGVDTIFEVDTIKSILEAVEKLTGVKYGENPKNDISIRII
                     TDHIRAVTFLVSDGVLPSNEGRGYVLRRLLRRAARHGKLLGVKELFLQKLIDEVIKVN
                     DKAYPVLVEKESYIKKVVGIEEEKFNETIDQGTEILNSYIEVLKNEGKTVLSGQEAFK
                     LYDTYGFPIDLTKEILEEEHLSVDEEAFNEEMEKQKERARNARGNMDGESWKEDPLSK
                     LESTVDSTFNGYSEIYGEGTIEAIVKDDELVQSAEEGDKVSIVLDNTTFYPEGGGQVG
                     DCGLITNENLVLEVLNTKKGANNSIKHIGIIKSGRISNGDKVKTLVDRETRMSAARNH
                     SATHLLHKALREVLGEHVNQAGSLVTPERLRFDITHFEAISNEELKVIEEKVNNVILS
                     SLDIKCDIMNIKEAKEKGATALFGEKYGDEVRVVSMGDYSTELCGGTHLTNTSQVGMF
                     KILSEGGVAAGVRRIEAITGKAVYEYLKERDGIISEVCVNLKSKEDNLIQRISSLLEE
                     NKNLSKELHDMKAKMSLQSVDSIFDSKVEVNGVNLITNKFEGMDMDTLRETADNLRDK
                     LGSGVVVLANVVDDKVNFVVTATKDVLDKGIHSGNIVREVAKIAGGKGGGRPNMAQAG
                     ASDVSKVDQALSYASEVIKTQVK"
     misc_binding    complement(15024..15262)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(15405..16484)
                     /gene="mnmA"
                     /locus_tag="PC700_000998"
     CDS             complement(15405..16484)
                     /gene="mnmA"
                     /locus_tag="PC700_000998"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDF2580318.1"
                     /translation="MNKKVMIGMSGGVDSSVAAYLLKQQGYDVIGVTMKLWQDDDVVE
                     IEGGCCSLSAVEDARRVANKIGIPFYVLNFREVFKEKVIDYFIDEYLEGKTPNPCIAC
                     NKHIKFDDFYKKARQIGCDYVATGHYAKIEKDESTGRYLLKKSVTDKKDQTYALYNLT
                     QEQLEHTLLPIGDYEKDRVREIAKEMGMAVHNKPDSQEICFVKDNDYANYVKKHSKKR
                     IEEGFFVDTKGNILGKHKGILYYTIGQRKGLGITFGKPMFVIDINPINNTIVLGDNED
                     LFKKELIAKDVNFISIDTLEEPLRVQAKIRYSAKPSPATIHRVGEDTIKIVFDEAQRA
                     ITKGQSVVMYDGDIVVGGGIIEKSL"
     gene            complement(16773..17213)
                     /gene="nifU"
                     /locus_tag="PC700_000999"
     CDS             complement(16773..17213)
                     /gene="nifU"
                     /locus_tag="PC700_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HDF2580319.1"
                     /translation="MQYSDKVMEHFMNPRNMGEIDNASGVGEVGNPTCGDIMKIFLDI
                     DGDVIKDVKFKTFGCGSAIASSSMATEMIKGKTIKDALELTNKAVAEALDGLPPVKMH
                     CSVLAEQAVKAALIDYAQKNNIHIPELDGYVIDDAHDHDVEEEE"
     gene            complement(17267..18460)
                     /gene="nifS"
                     /locus_tag="PC700_001000"
     CDS             complement(17267..18460)
                     /gene="nifS"
                     /locus_tag="PC700_001000"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HDF2580320.1"
                     /translation="MEKRRLYMDYSATTPIKKEVLDAMMPYLTDYFGNASSFHTFGRE
                     AKDALDKAREQVAALINAEPSEIYFTAGGSESDNWTLEGVAYANKNKGNHIITSKIEH
                     HAILHTCEYLAKHHGFEITYLDVDSEGRVDLRQLEDSIKDTTILISIMFANNEIGTIQ
                     PIKEISEIAKKHKILFHTDAVQATGNIPVDVKELGIDLMSMSSHKIYGPKGVGALYIR
                     KGVRLHNFVHGGAQEKSKRAGTENIPAIVGYGKAAELAKENMQNHVETLTRLRNKLID
                     GVLERIPYTRVNGSLENRLPGNANFAFQFIEGEGILLLLDMLGIAGSSGSACTSGSLD
                     PSHVLLAIGLPHEIAHGSLRLTVGDFTTDDDIDYILENLPKVIERLRSMSPLYDDAKK
                     QGLVK"
     gene            complement(18478..18924)
                     /locus_tag="PC700_001001"
     CDS             complement(18478..18924)
                     /locus_tag="PC700_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDF2580321.1"
                     /translation="MKLSTKGRYGLKAMFELALNQDNGPVSLKFIAKKQKISDQYLEQ
                     IFSSLKKSGLVKSVRGAQGGYLLSKNAEDITVGDILVVLEGPVALSDCVLDEDVCENS
                     NMCVTKIVWEKMKKGIEDVIDSITLKDMINDYNKNKLENDITNIKK"
     gene            complement(19033..19803)
                     /locus_tag="PC700_001002"
     CDS             complement(19033..19803)
                     /locus_tag="PC700_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2580322.1"
                     /translation="MIIKLNSTYHSRVMKYLKKEPEYNLFIIGDIERYGYGNNFLNIW
                     ADVGEHGEIKAILLKYFEFMMFYSDGEYDVEGFYNLLRNTNYEEISGKICAVDTLAKR
                     LGLNNLKVVDFCKLQTKKFLIDNNCNAKVKRIRLGNLKKTVKLYDLIDEFHSTTLENL
                     KNGLRTGRGYCIEINKQVVSMAKSTSENRTHAMIIGVGTHPKYRAKGLATKCLIKLCS
                     ELLRENKIPCLFYDNEEAGKIYKKLGFENIGKWGIYSK"
     gene            complement(19834..21099)
                     /locus_tag="PC700_001003"
     CDS             complement(19834..21099)
                     /locus_tag="PC700_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HDF2580323.1"
                     /translation="MPLADKIRPKTMNDVIGQSHIIGNGKILSKILQSNFLPNMIFFG
                     PPGVGKTTVAEIIAERSNKSFYKINATNSSLEDIKKVISELGSINNVNGVLLYIDEIQ
                     SFNKKQQQSILEFMENGSITLIASTTENPYHYVYKALLSRSTVFEFKPLEKLDIEKGL
                     KRAVEVLNEDSYMDIECNNNAIEDIAILSDGDMRRALNILEVVVYSTKPNKDNITYID
                     SDVIRASTFNKIINYDKNGDSHYDILSAFQKSIRGSDPQASIHYLARLIKGGDLISIC
                     RRLLVIASEDIGLAYPNAIVIVKACVDSAMQLGFPEAKIPLAEATILLATSPKSNSAC
                     IAIMKAMDELDKEFVKDIPNSIKDAHYSGSKEIGRGCGYKYPHNFKNHYVKQQYLPDS
                     IKDSIYYIPQENKTEKNIKKYLEYLNSDF"
     gene            complement(21206..21991)
                     /gene="codY"
                     /locus_tag="PC700_001004"
     CDS             complement(21206..21991)
                     /gene="codY"
                     /locus_tag="PC700_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HDF2580324.1"
                     /translation="MASEVLQKTRKINKTLQTSGGSSVSFDLLAGALGDVLSSNVYVV
                     SAKGKVLGLHLNDVQDSSVIEDEYTKQKKFSDEYTQNVLKIDETLENLNGEKILEIFP
                     EEHGRLQKYTTVVPILGSGQRLGTLVLSRYSNSFNDDDLVIAEYSATVVGLEILRAIG
                     EELEEEMRKKAVVQMAIGTLSYSELEAVEHIFAELDGKEGLLVASKIADRVGITRSVI
                     VNALRKFESAGVIESRSLGMKGTHIRILNDKLTDELKKLKNNQ"
     gene            complement(22156..24243)
                     /gene="topA"
                     /locus_tag="PC700_001005"
     CDS             complement(22156..24243)
                     /gene="topA"
                     /locus_tag="PC700_001005"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDF2580325.1"
                     /translation="MAKTLVIVESPAKAKTIEKFLGKSHYTVKASVGHVRDLPKSKLG
                     VDIENNFEPQYINIRGKGDVIKELKKEAKKSKQVYLATDPDREGEAISWHLAHILNLD
                     ESEDCRIEFNEITKDAIKKAIKHPRNIDINLVDAQQARRVLDRLLGYQISPILWQKVR
                     KGLSAGRVQSVTTKLICDREKEIKAFIPKEYWTIDVEAKTVKDKDITLKFYGKNDEKI
                     ELENEEAVNEILKEMEGKDLKVEKIESKSRRKSAPKPFTTSMLQQEGANKLFFTTKKT
                     MMIAQELYEGIDIEGEGTVGLISYIRTDSKRISDEAKEKAKGYIVNNLGENYYKNTGD
                     KKEKKDTKKVQDAHEAIRPTSVDRTPESIKASLSKDQYKLYNLIWRRFVASQMEDSVF
                     DILNVECKIGDIVFKATGSKMKFDGYTKVYNFSEREDKILPSIEKGDILNIENILPDQ
                     HFTQPPARYTEASLVKTLEELGIGRPSTYAPTIATILNREYVEKQGTSLCPTELGIIV
                     TDILENNFHKFIDVDFTADMESKLDVIEEGNTDWKKIVSESYAPLKEAIEEALENIEK
                     VNMDEETDEICENCGSNMVIKYGRFGKFMACKNYPDCKTTRPIMNKIGVKCPKCEEGD
                     IILRKSKRGKAFYGCSNYPNCDFVAWNKPTGEFCEKCGSYMVEKVTKSETKTVCSNKE
                     CNKETKEHNDGKE"
     gene            complement(24276..25376)
                     /gene="dprA"
                     /locus_tag="PC700_001006"
     CDS             complement(24276..25376)
                     /gene="dprA"
                     /locus_tag="PC700_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDF2580326.1"
                     /translation="MEKRDTYLWLKSIGGITTKTIEIIENEIVNIEDIFDFSEKEIYN
                     LKNISLNIRKNIVKYRGYAYLENIKELLYKKAIKYICKYDKEYPENLKNIYNAPKLLF
                     YKGDIGLVNNNFNIAIVGSRKPTAYGINCAKTISCQLSQYGVNIVSGLAIGIDAYSHI
                     GCMSGKSKTIAVLGSGVDNPLPKQNLHLSNKILENGGLLLSEYNINSTVAPYHFSNRN
                     RIISGLSDGVVVVEAAIKSGALITVDFALEHGKNVFAIPGNINSQMSRGCHKIIKEGA
                     KLIENIDDILNEYNIFNIIDKKINQKYDNISLNAKSKQIIEAIKREGNLHIDSICDYT
                     GIEIKYVNSIINELVLNELVVEMNNKTYSLNV"
     gene            complement(25395..26996)
                     /locus_tag="PC700_001007"
     CDS             complement(25395..26996)
                     /locus_tag="PC700_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HDF2580327.1"
                     /translation="MLSIINSSNLVGIDSFLVKVEVDVSNGIPSFNIVGLPGKEIKEA
                     RERVKSAILNSGYKFPSTRIVVNLSPADIKKEGAFLDLSISIGLLRELIKKDENYIRE
                     SMFIGELSLDGKIRKVRGILPIIMGAKTQNIKRIFIPIENIKESLLVDEIDIIPIKSL
                     KECVDFLNEEIKVDKVSIMSFLDDKSRKENGELEKDNSYIDCKYTKINNEESKYDEDF
                     KDVKGNYFVKRSAEIAAAGNHNMFMIGPPGSGKTMIAKRVRTILPDISIEEMIEVSKV
                     YSILGMINESKGIIDKRPFRAPHHTTTKQSLIGGGMDARPGEIALAHRGILFLDEIAE
                     FDRKILETLRQPIEDGYVNISRVKYSAKYPCRVLLVAAMNPCPCGYYMSETECRCRSN
                     EIDRYINKISGPLLDRFDIFVEVNSIKYSDFNSLKQEESSQKIKRRVENARKIQINRF
                     KKDNIKNNSEIKAYNLFKYCKLEKEASKTAEMIFNKYNLSSRSYTKLLKMARTIADLE
                     ERDLINSQCIIEAFSFRKAYYSYFK"
     gene            complement(27027..27371)
                     /locus_tag="PC700_001008"
     CDS             complement(27027..27371)
                     /locus_tag="PC700_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HDF2580328.1"
                     /translation="MNNKEKGDFGEEIAVNYLLSKGAKILEKNYRLRIGEVDIIANIG
                     DEVVFVEVKSRSNIKYGYPCESVDFKKRKKIIGVAKYYIIKNNLNNTPIRFDIIEVYL
                     LEKRINHIMNAF"
BASE COUNT         8594 a         5018 c         2906 g        11244 t
ORIGIN      
        1 acacaacaac ttagagcatt ttaaatcttt taaaatttaa aattacatac taaattattg
       61 tgattttata tttttattca tttgttgcca cttcttgata attttctata ggcggcaatt
      121 cttcaagact ttctatattc aacaacttta aaaattcatc tgttgtttta taaattattg
      181 gtttccctat cttattcaat ctaccagctt ctcttataag ttcattttca aacaaagttt
      241 gtaatacctt atcacatttt actcctctta tatcctctat ttctaccttt gttattggtt
      301 gtttgtaggc aattatagta agagtttcta atgtagcttg gctcaaactt ttctttttct
      361 ttggttcaat aatcttcttt atatattctg catagtcttt atttgtacac atttggtact
      421 tattctctag cttgattatt tgaatacctc tattttgttc tctgtactcc tcaataagta
      481 aatttaacat aatctctatt tcttttgatg aaagttcttt atttattata tagtttaatt
      541 cttttatact tattggttcc ccatatgcaa acataacaga ttctataata tactttatat
      601 cttctctttt cattttaaca ctccgaactt tttcttatta gaatatcatc aaaaaatatg
      661 tcttgtacta ctactatttc tttttctttt ataagctcta atatagataa aaaagtagct
      721 attatttcat cattttcata tgattttata atatttgtaa aagtaacttc aatcttatct
      781 tttataatat ctcttatata caatatcttt tcttccacag atattatctt cttccttact
      841 attttgtcta gtttctcatc attagcattt tctatctgtg atgttttaac tttaaatatg
      901 tatggaagta tattttttat tgcttcaatt gatatatctt ctaaatccac attgtcatca
      961 ataattatct cttctttatt tctataaaat ctctcattaa cataagttat attttctttt
     1021 atatcttgtg atgctacctt aaactttcta tattcttcta acttttccat aagttctatt
     1081 cttgggtctt cttcttcatc tttttgctta taaagcagat atttagactt tatctctaaa
     1141 agttttgatg ccatagttat aaattcacta gttatctcta aatccatttt atctagcatt
     1201 ttcaaataat ttagatactg cttagttata tctattatag atatatcctt tatatctatt
     1261 ttatgctttg ttattaaatc ataaagtaaa tctaatggtc cctcatagac ttgtaattgt
     1321 atattatact tcatatcaat ccctctttaa tctgtttatt atattaaaca aataaatata
     1381 ctatttttag caacactgta tatattggtg atagtattat ttgatatata ccagtaaata
     1441 aagcaattaa taaaattata taactcatac tttcaaattt atacaaatat tcattccatt
     1501 tatatggtac aaatgtagat ataactcccc atccatcaag tggaggtatc ggaagtaagt
     1561 taaaagctgc aaatcccata ctatatataa gcacatatcc agctattgta tttatagcat
     1621 acatatgcac atacttattg ataattacac acagtatagc agtcaaaaca ttacaaaaaa
     1681 cgcctgcaag tgaaactatc aggtttccta tcttatagtt tttaaaatta tttggattta
     1741 ctggtactgg ctttgcccac cctatcttaa ggataaacat agccaaaaga cctattggat
     1801 caacatgtct tacaggattt aatgtcattc taccataagc tttagctgta tcatctccca
     1861 ataagtgagc tgaataagca tggccaaact catgtattga tattgccata gctataccaa
     1921 ctaaacttgc tactatttct cctatattca tacttaacat taatgtacat ctcctaaatt
     1981 aaatttttaa tattattata acaattaaat agcttataat caaaattatt ttacattata
     2041 ataattttaa cttctaggca taaaattaaa atttgctttt tattttataa cttttattat
     2101 tatatcatat tcattgtgaa aatataataa ttacacctac tgtaagtgta atattatatt
     2161 gacatgaatt gacattattg tctcttagtc aatattatac aagaagtata aaaaaatatg
     2221 tgttacactt tgtataacat gaaaggaggt tacctaagtg agacaagaag aacgcacagt
     2281 atgctttgat agggaattaa aaattgaagc atatagattt aaaggcataa tgcaaaaatt
     2341 cccaaaccat tttcatgaac attatgtaat tggttttata gaaaaaggtc aaagatattt
     2401 atcttgtaaa aataaagagt ataccacaag tactggagat ttgctacttt ttaacccttt
     2461 tgacagtcat acttgtgaac aaatagatga taaagtactt gattacagat gtatcaatat
     2521 taagccagaa ataatgaaaa aaaccgtctt tgaaattaca ggtaaaaatt atttaccaaa
     2581 atttaatcaa cctgttatat ttcgcagtga gctagtacct ctactacaag aattacacta
     2641 tataatcatg gaagaagaat tggattttaa aaaggaagag ctttttttct tcctaattga
     2701 gcaattaatt gaagaacata cagagccaaa tttgcaatct aacttggaaa atacaaatat
     2761 agaaattcaa gcagtatgtg attatctaga aaataattat gctgaacata tagtacttga
     2821 cgaattaagc actatagcag gtatgaataa gtactctcta cttcgtaatt ttacgaaatt
     2881 aaaaggtatt acaccatata gatacctaga aaatatacgg gtaaacaaag ctaaaaagct
     2941 tttagaaaaa ggcgtagaac ctatagatgc tgcgatacaa acaggatttg tagaccaaag
     3001 tcactttaca aactttttta agaattttat tggtcttaca ccaaagcagt atcaaaatat
     3061 atttataaat gatgattcaa acaactttaa ataaggtaaa aaggagaaat aaaaatggaa
     3121 aataaaaatt caaaatgtgt catggtaata gacgaaaatt taccaatggg aattatatcc
     3181 aatacagcag ctataatggg aattacacta ggaaaacatg ccccagaaac tgttgggcca
     3241 gatgtaattg acaaaacagg taatagccac ttaggaatta ttgacattcc tgtacctatt
     3301 ttaaaaggaa acaaagaaat aataaaagat ttacgtaaaa aattatatac tttggaattt
     3361 aacgatttaa ctgttgtaga tttttcagat gtagcacaaa gttgcaatct ttatgaagaa
     3421 tttacacaaa agattgctag tgttccagaa gatgagttac agtattttgg gatagcaatt
     3481 tatgggaata aaaagaaagt gaataaactt actggtagta tgcctttact tagataattg
     3541 ttaaacaacc ctatatatga tattaaatca aatgatattt taagatacat aaacttttct
     3601 atatttctat aaaaatcata agattataaa atacttctat atggtaaata aaaatacccc
     3661 tatggcaaat aactacataa gggtattttt tatgtgtatt ataatcatat ctttgtgtat
     3721 tataattata tttcatctga taattattta tatcatatta tcaattattc tttgaagcat
     3781 ttgtagatag cttgccttat ttatatcctt agtatttatc aattccactt cgcctattac
     3841 ttttttatct ttacatatct ttatttttcc taaaacagtt ccctttttaa ttggcatctt
     3901 aggatttttt actatttcaa ttttcttctc aaattcttta gagcttcctt ttttaataag
     3961 agcatttaaa tcttctttag caaccaattt aactttgttt tcatcagcct tatccaatgt
     4021 aagtgttgct atattatcac ctttactaca taacttaaca ctttcataat tagcaaatcc
     4081 atagtttaaa aggctagagg catcattaaa tctttctgga gaagtctctg ctcctaaagt
     4141 tgcagcaact agatgagtat tacctctctt tgcagatgct gataaacagt attttgcttc
     4201 ttgagtaaaa ccagttttaa caccagttgc tccttgataa tgctttataa gtttattagt
     4261 atttgcaaga cctactgtaa cttgtttctt ccctactaca accttatcca tccatgtagt
     4321 taagtattta cttatcattt cgtgttttaa tagttctcta gacattaaag ctatatcatg
     4381 agcagatgta tagtggtttg caactggaag tccatttgta tttgcaaaat gagtatcatt
     4441 catatttaac tcttttgctt tagcattcat tctatcaaca aatccttcaa cacttccagc
     4501 taagtgttca gccatagcaa cacaagcatc atttgcagaa gcaacagcta taccttttaa
     4561 taatgtatca accttttgag tttctcctgg ctctaggaat atttgactac cacccatact
     4621 agatgcagtt tcacttactt gaacttcatc atcaagctta atcttgcctg aatccaaagc
     4681 ttctactata agtagcatag tcattacctt agttacacta gcaggaggaa gctttttatg
     4741 agcatccttt tcatataaaa tttgtccact accaacatcc atcaaaattg ctgattttga
     4801 agatatattt agttggttgg catcagcatt ttccttatta tttgcaaaag aaaagcttaa
     4861 aggagtaagc gcaattaata tagccaaagc agtactagct aattttttca tcgatgtcac
     4921 ctcttaaagt ttatttaaac ttattttctc acatttaact ttttttattc taaatgccta
     4981 gatatacctt gacctaaatt ttttttcttt tcaagctttt taaagtaacc ttctaaaaca
     5041 cctttattag acgcattttc tattatttca aaagcatctt cataattcat tccatactct
     5101 cctgctattt ccatttttgt ttgttttaaa gcttttatag cattttgtct tgaaacatca
     5161 tccataagaa tcctcctatc agtaaaattt atatataagt agtttttact aataaaagat
     5221 tgttataatt ggtattttat gtaatatata ttttttttaa atgagtatta aaaagacctc
     5281 ttgtaattct taagataata tcaattggat ttttgatatt actttaaaaa ttacaagagg
     5341 tctaattttc aaatttatag tttttttata ttatacttcc attatgatag gaagtatcat
     5401 tggatttcgt ttagtttttt gatataaata ttcttttagg ttttctttaa tattattctt
     5461 taaataagcc cattctttaa tatttttctc ttcacattca tttagaacat tcttaattat
     5521 atcctttgct ccatccatta aatcttctga ctctcttaca tatacgaatc ctctggaaat
     5581 tatatctgga cctgctaaga ccttaccctc atccttggaa atagtaacta caactatcat
     5641 aagaccatct tctgataaat gctttctatc ccttaaaact atattaccta catcgcctac
     5701 tcctagacca tcaactaata tgtttcctgt ttgtatacgg tttgctacct tagcagagtt
     5761 tttatctaat tctaaaacat ctcctgtttg catgacaaaa atattttctt tatccatacc
     5821 aagctgttca gctatttcag catgtctttt taacattcta tattcaccat gagctggcat
     5881 aaaaaacttt ggtctaacta atgcatgtat aagttttaat tcttcttgac aggcatgtcc
     5941 agaaacgtgt atatcagcta tatcactgta tactacttct gctccttttt caaataagaa
     6001 attgattact cttgatatta atttttcatt tcctggtatt ggatgagcag atattataac
     6061 taaatcgcca ctctttattt ctactttttt gtgctctgaa aaagccattc tagcaagagc
     6121 tgacataggt tcaccttgtg aaccagttgt tattatcact aattcactat cttcatactt
     6181 acatatatca tttaaatcta taagcatgtc atcagaaata tctaggtatc ctaattcttt
     6241 tgcaactccg actacattga ccattgagcg acctgatata gcaacttttc tattaaactt
     6301 ttcagcagta tttattattt gttgtaatct gtgtatgttg gatgcaaagg tagcaactat
     6361 aattcttctg ccatttcctt ttgcaaataa ttcgtcaaga cctaccccaa cagtcttttc
     6421 agacatagta aatcctggtt tttctgcatt agtactatct gccagcatta aaagaactcc
     6481 ttttttgctc aactcacaga ttctatgcat atccataaca tctccatcaa taggagtcaa
     6541 gtcaatctta aaatctcctg tgtggtatat tataccttgg tctgtgtgta ttgcaataga
     6601 gtaagcatct ggtatactat ggttattttt caaaaactct acttccatat tatcaagctt
     6661 tataacttgt ctaggaccta taacatttaa cttaacatta ttcattttat gttctttaag
     6721 ttttacttgt ataagaccta tactaagtct tgccccatat acaggtacat ttattttctt
     6781 taatatatat ggaagagctc ctatatggtc ttcatgtcca tgtgtaataa atataccctt
     6841 tattttatcc ctatttttta ctaaataagt tatatctggt ataactatat ctactcctaa
     6901 catttcatcc tctggaaaac taagtccagc atctataaca attatttcat ctttatactc
     6961 aacaacagtc atattttttc caacttcgtt aagtccaccc aaggccatta cctttatctt
     7021 gttggtgttc tttttgaaca attgcatcat ctccttctgt ctatttaatt ttttaacacg
     7081 ttctacaatc ataaataata tcaaatcacg cactcgctac cagttattct tattgtacca
     7141 taattcgtat catattttaa gctatattta aaatacattt aacgtattat tttgacaaac
     7201 ctattttatt ttgatatcct ctaaatatta tatgatttaa tatcggataa tttaggagga
     7261 tttatgcgtg gtaaaactca ttgtgcaata ggaatcttaa cagctatcca aacatcttta
     7321 atatttaaaa ttcctatatc tttagttgat attttagttt cagctacttt tgctgtacta
     7381 cccgatttgg ataaatctaa ttctatggtc tcaaatttca ttctaaaaaa caatgtatcc
     7441 aaatacatat atagaatttt tatatatgct gttaatatta ttattttctt tatttcaata
     7501 aatataaatg ataattttta tttaagcgca ataattacat ttgttgcaat tataattatt
     7561 gaagcaaaac taacccacac atttctaaga aaagtttttc tatcattaat atttatacta
     7621 ttagctatat gcttatacat catagaagtt gaaatatatt ttacaatttt ttgtctaatg
     7681 ttgtcaattt ttccctggtt aaagcacaga agtttttctc atagcatatt tgcaactata
     7741 atagtatatt ttttactaaa acagatagaa ttaatcacta atattaataa tttatctttt
     7801 tatggtacga ttggttatgc aagtcatatg tttttaggag atttatttac aaagcaagga
     7861 ataccaattt tttatccgct aagcgaaaag aaaatttctt taggtttttt aactgttgga
     7921 ggaccattta gtaatttcat agaaaaatca ttcatttttg tacttatagg attaattata
     7981 ttttcaatat taaaactata agcatcttga aaactgtata tttgcatttt gtagatggtt
     8041 tataataaaa aatggaagaa tagcatagaa tataaaagtt ctatactatt cttccatcga
     8101 cattaagcaa tagaagttta ttcttctgaa tcgcttactc cattacactc gtcacataat
     8161 ccataaaatt taacatcatg atcaagtatt ttaaatttgt acaatgattg tatctctttt
     8221 tctatattgt ctaaaaggtc ttctttagct tccattatct ttccacaatt tttacaaata
     8281 agatgatgat gattatgaca gtcttcttta tgtagactaa tctcatatct tatacatcca
     8341 tcgtctaagt ttagttttga tattaatcct atttcatcca atagctgcat tgttctatat
     8401 acagtagcta agcctatttc aggacagtca accctaacta aatcatagat ttcctcacta
     8461 cttagatgtg aatggtcatg tttaagcaat atttcaacta ttgctcttct ttgtggcgta
     8521 atcttaaaac cagtttcttt taacttgtct tttaataaat ccattgtatt tgccataaca
     8581 tcacccttac tattttcaat tgataatctt tattaaataa tattgtaatt gataattcat
     8641 tttttgtcaa gacatttaaa taaatttaat ttattctcat aatataaata tatacccata
     8701 aaagatatat tattcaccaa aattcataaa ttttttggta tttttaataa aaaacaaaaa
     8761 aacagatata tactataaat gcttttacaa ttaataaggt ttaattaaag caaatataat
     8821 aaatatctga tttttatata caaacttaat tacttatcta gttaagtcca tttgattcat
     8881 cttcgtcgta atttaaacct tcttcttcca ttatagcagt atacacagct acaacagttt
     8941 catactcttc atcactttcc aaaggtatta atatttcacc ttcttcatcc tcatcaattc
     9001 taaagattaa agcttcttca gcttcttcat catctaaagg cattaatatt gcatactctt
     9061 ttccttctgc ttctaatgtt aatattattt caaattggct ttcaacgcca ttttcgtcta
     9121 ttaaatttat tatattttct tccataatgt cattcacctc aaatttattt ttatttttat
     9181 tatttatatt atatatcata agtattcaat tttatagttc ttttatgaat ttattttaaa
     9241 atctaaatat ccttgcaaaa ttattactgc tgccaacata tctataacct tttttctttt
     9301 ttgtctacta acatctgctt ctaataaaga cctctctgcc gctactgtag aaagcctctc
     9361 atcccaaaac tctacatcaa tacctgtttc tgcttttacc aattcacaaa acttgattac
     9421 tttttctcct tgtggaccta aagttccatt catatttttt ggtaatcctg atacaatttt
     9481 atttacttgt ttttctttta ttattgcttt taactcttca atatcttttt tcttgccaac
     9541 tctttttatt gtttttacac cttgtgcagt taatcccatc aaatcactca cagcaactcc
     9601 aatagtttta tcaccaacat caagacccat tattcttcca tctaacatag ttcctccttt
     9661 atctatttat attctataga ttaaatacat attctgaacc tctcataact aaggtcacag
     9721 gcttcctgct tcatagaatt ttgcataatc aatcataaaa attgcaatat gattttttat
     9781 gtaagcatcc agtttacatt aataatcata ttgcattttt tatttaaatc ctatatgaac
     9841 agtaaaaaat tttttagttg tttttttcaa atattatcat atttatattt attattatta
     9901 gtggtataag tatatatata gatgctgtag atgcagcaac tattccagaa tcattaacta
     9961 aaagagtaat tatacaacca accatagatg caataaaccc tttaaacaag attggaaaat
    10021 tattcattat ctttctaaag tgtctagatg gtttaaatat aagtactgca attactccaa
    10081 ttcctacaag taaaatattt acccatacac ttgtctgtgc tagttgcata ttcatttgaa
    10141 tttttcttcc aaatgtttgt aatatctctc ctggtccatt taggaaaatt ctctgtacaa
    10201 acatacctaa atgagattca ctacctgaaa gtatatctaa agctgcaaat gcaaaaacga
    10261 caactacagc tgaaacagct aataatacag ctttcttaaa atctatttta atgtcatata
    10321 taagcattat aaacaacaaa tatgctacac attctgatat agctccacca acatttgcac
    10381 ccattgaagg atatgcacta gttataagta ttattaacga aaaaattaca acaagtaatt
    10441 tagatacctt cttataatgt aataatactg ctaagccaaa tactgcactt gctatagtta
    10501 caccttcata ttcatttcct acaccatagt acctagctcc tatgacagca tcataactca
    10561 ttatgttatt ttgcattaag tatgttccaa atacagaatc tataactatg actaaaatag
    10621 taagtgttgc aaaaaatccc atgtgcttta tatcattgtc cttaaataat tttctaccta
    10681 acaagtataa tattagcgta gtcgcaaata ttcccaaaga tattgaaatt ggagttttaa
    10741 aattcatcaa aggagctaac ataaatgata gtggcattat tatgcctaat tttataaact
    10801 ccttaagtac tttgaataca cgctctttat gtggcatata atttctaaag aaaatcgcaa
    10861 acatggcaat tacccaagaa gcagatatta cgcctacaaa gccatttata atagttgacc
    10921 ttattgaaga tatagacact atcctttcat aaagtgattt tagatagcca acattgtcct
    10981 ctttatttac taaagtataa cttctaccta ccatctcttt attttcaaga ccaaatgtac
    11041 ctagtatatc tactccaaca tctaaattgg caacaattcc agcctttctt gttgtggcag
    11101 attccagtag acctttccct tctccactaa attttattat tggagaaagt cttctcttat
    11161 tcttataatc taggtcactt ggaaatgcac ttgctatata aacagtgtca ttttctccaa
    11221 ccatagaaaa tacttgttca agatatttat ttatattttt tacaatattt ttcttcattg
    11281 taccataagt attatcattt agatttaatc tatacatatc taatctatat gtatctccaa
    11341 gttctacaaa tatagcatca ctatttttat aggatttttt agtttcatct agtaatttat
    11401 cgtagtctgt agatattcca aatggcattt tagaatcttt cttattaata ttatcaatat
    11461 tacctgaatc aatccttcca tattggtcca tagctactaa acataagttt ctatttttaa
    11521 ctaattgatt attttcaatt atatcagcat ttccaattac agatgtcttt aaaccatttt
    11581 ctgataaagt ttttcctaat gaccctaaat ttgctccata ttctccatac tcaagatttt
    11641 catttataga tctattaatg cctaaatcat ttatttcttt agctttttga cctgttgcga
    11701 cttcaaaagc tttagcagta tcttggtcta ctttttgaaa attgatatag tcttcagtag
    11761 ttacactagc tcttgttcct gcacccatac ttgcataaga ccttctatca tcagtacctt
    11821 gatcccctct tatattcatg agagctacat agcctctctt ttctatctca tttttcaaga
    11881 ttggaatatt catcatactt ccaagattgg tcctattcat atcaataaat atgaccttgc
    11941 ctttttcttc agcaaaacca tagctagcag ttgatacaaa aatcataatg actattgcaa
    12001 acaacgacat aatctttttt ctcatttatt tgacctactt tctattcaga tagtgcgtga
    12061 ttacttcttc aagtatctca tctctttcaa attttttaat tatagctctt gcatttttat
    12121 gactagttat atagctagaa tcaccagaaa ggagataccc tataatttga tttatagggt
    12181 tatatccttt ttcaactaat gctttatata caaaatcaat agtatctcct acactcatcc
    12241 tatcttctgg tattccttca aatttcatag tgtagtctaa atctttttcc aaactaaaaa
    12301 cctccttgag tgaatgttat ttaacttgtg tcttaataac ctcacttgca tagcttaaag
    12361 cttggtcaac cttagataca tcactagctc ctgcttgtgc catattaggt cttcctccac
    12421 cttttcctcc agctattttt gcaacttctc taacaatatt acctgaatgt attcccttat
    12481 ctaatacatc tttagtggca gtaactacaa agttaacttt atcatctact acatttgcaa
    12541 gtactactac accagaacca agcttatctc ttagattatc tgctgtttct cttagtgtat
    12601 ccatatccat accttcaaat ttattagtta tcaaattaac tccatttact tcaactttag
    12661 aatcaaaaat agaatcaact gattgtaaac tcatcttagc tttcatatca tgcaattctt
    12721 tagataagtt tttattttct tctaataaag aacttattct ttgaattaaa ttatcttctt
    12781 tagattttaa gtttacacaa acttcagaga taattccatc tctttctttt aggtattcat
    12841 aaacagcttt tcctgttatt gcttctattc ttcttacacc tgctgctact ccaccttcag
    12901 ataatatttt gaacattcct acttgagaag tatttgtcaa gtgagttcct ccacaaagtt
    12961 ctgtagaata atctcccata gaaactactc ttacctcatc accatatttt tcaccaaata
    13021 gtgctgtagc tcctttttct ttagcttcct ttatattcat aatatcacat tttatgtcta
    13081 aagatgataa aataacatta tttacttttt cttctattac ttttaattct tcatttgata
    13141 tcgcttcaaa gtgagtaata tcaaatctaa gtctttctgg agttactaaa gaaccagctt
    13201 gattaacatg ttctcctaat acttctctta atgccttatg aagtaaatgt gtagcactgt
    13261 gatttcttgc agctgacatt ctagtttctc tatctacgag tgtctttact ttgtccccat
    13321 tacttattct tcctgatttt attataccta tatgttttat actattatta gcaccttttt
    13381 tagtatttaa tacttctaaa actaagtttt cattagttat caatccacaa tctccaactt
    13441 gtcctccacc ttcaggatag aaagtagtgt tatctagtac tatagatacc ttatcgcctt
    13501 cttctgcact ttgaactagc tcatcatctt taactatagc ttcaatagtt ccttcaccat
    13561 aaatttcact atacccatta aatgtactgt caactgtaga ttctaatttt gatagagggt
    13621 cttccttcca actttctcca tccatatttc ctctagcatt tctagctctt tctttttgct
    13681 tttccatttc ttcattaaaa gcttcttcat ctacagatag atgttcttct tctaatattt
    13741 ctttagttaa gtctataggg aagccataag tatcatatag tttaaaagct tcttgaccac
    13801 ttaggacagt ctttccttca ttttttaata cttctatata agagttcaat atttctgttc
    13861 cttggtctat agtttcattg aatttttctt cttctattcc aactactttt ttaatataac
    13921 tttctttttc aactaaaact ggatatgctt tatcatttac ctttataact tcatctatta
    13981 gtttctgtaa aaataattct tttactccta ataacttacc atgacgagca gcacgtctaa
    14041 ggagtcttct aagaacatat cctcttcctt catttgatgg aagcactcca tcactaacta
    14101 agaatgttac agctcttatg tggtcagtta ttattcttat tgatatatca ttttttggat
    14161 tctctccata ttttactcct gttaacttct caactgcttc tagtatagat ttaattgtat
    14221 caacttcaaa gatagtgtct acgccttgca taatacatcc cattctttca agacccatac
    14281 ctgtatctat attcttgttt tctaatagac tataatttcc ttcttcatct ctatcaaatt
    14341 gagtaaatac atggttccaa aattctaaat acctatcaca atcacagcct ggtttacagt
    14401 ctggattgtc acatccatat tcttctcctc tatcaaaata tatttctgaa caaggaccac
    14461 aaggacctgt acctatttcc cagaaattat catcttttcc aagtctaacc attctttctt
    14521 ctgggaaatt catttctttt gcccatatat cataagaatc atcatcttct tcatatacag
    14581 tcacccatat cttatcttct ggtatattta gccattctgt aacaaattcc catccccatt
    14641 tgattgactc tcttttaaag taatctccaa atgagaagtt acccatcatt tcaaagaatg
    14701 tagcatgtct tgcagttata cctacatttt ctatatctcc tgttcttata catttttgag
    14761 atgtacacat tctcacactt ggaggtactt ctacacctga aaagtagttt ttcaaaggtg
    14821 ccatacctga gtttataagt aacaaactct tatcattgtt tggtactaaa gaataactat
    14881 ttgctacata atgacctttt gactcaaaaa attctaaaaa cttacttctt atttcattta
    14941 atcccatctt ttccatatca tgttcctcct aataaatttt attttttcgt ttaaataaaa
    15001 aacctcgtcc ctataaataa atttataggg gcgaggacaa tcgcggtacc accctaatta
    15061 tcatacaaag tatgatatct tcggaaaacc ttagctataa cgtaactacc gtcaatctct
    15121 acttaaatct tctttataaa agataaattg ttcaagattg ctactaaaag gctgcttcaa
    15181 atatcatatt ctaagggctt ccagcatttc cctctctctg taagacatca aatattttac
    15241 tattccttat cactgtattt gttttttaat taatatacta attatattac taaattttac
    15301 aaaattaatc aatactatat tttaatatac aataaattta ttttataaac tttatttact
    15361 cttaatatac tttattgctt atttttttaa ttttttagat gtttttataa acttttctcg
    15421 attatacctc cacctactac aatatctcca tcatacatta ctacagattg acctttagta
    15481 atagctcttt gagcttcatc aaaaactatt ttaatagtat cttctcctac tctatgaata
    15541 gtagctggtg atggttttgc tgaatatctt attttggctt gaactcttaa gggttcttct
    15601 aacgtatcta ttgatataaa gttaacatct ttagcaataa gttccttttt aaataaatct
    15661 tcattgtctc caagaactat ggtattattt attggattta tgtctattac aaacattggt
    15721 tttccaaaag taattccaag cccttttctt tggcctatag tataatataa tataccctta
    15781 tgttttccaa gtatattgcc tttagtatct acaaaaaaac cttcttcaat acgcttttta
    15841 gaatgttttt ttacataatt tgcatagtca ttatctttaa caaaacaaat ttcttgacta
    15901 tcaggcttat tgtgtactgc catccccatt tccttagcaa tttctcttac tctatctttt
    15961 tcatagtcac ctattggcaa taaagtatgc tctagttgct cttgtgtaag attataaagt
    16021 gcataagttt ggtctttttt atctgtaaca gattttttta gcaaatatct tcctgtactt
    16081 tcatcctttt ctatctttgc atagtgacct gtagctacat aatcacaacc tatttgtcta
    16141 gccttcttat aaaaatcatc aaattttata tgtttattac aagctataca gggattagga
    16201 gtttttcctt ccaaatattc atctataaaa taatctatta ctttttcttt aaacacttct
    16261 ctaaaattta aaacataaaa tggtatacct attttatttg ctacccttct agcatcttca
    16321 acagcagata aagagcaaca accaccttca atttcaacaa catcatcatc ttgccacaac
    16381 ttcatagtaa caccaatgac atcatatcct tgttgtttta aaagatatgc agctacagag
    16441 ctatcaactc ctccactcat acctatcatt acttttttat tcataaacaa atttcacctc
    16501 atcttttgca ttacttatta ttataccata tagagcaagt attgtactta tttaaaatat
    16561 agcttgttct attttctaaa aaaataatca tctttattat ttgaatctta tggattattt
    16621 tgttaactaa ttaacctcta aatcaagtta aaatacttaa tttaggcaat aaaaaatagc
    16681 cattttaaaa acagctattt tttatgtcct aattatatca ttagtacttc tttttaatca
    16741 ttgtaaactt ctcataacac aatcacttat ttttattctt cttcttcaac atcatggtca
    16801 tgagcatcat ctataacata tccatccaat tctggtatat ggatattgtt cttttgagca
    16861 taatctataa gagctgcttt tacagcttgt tcagctaaaa ctgaacaatg catctttact
    16921 ggtggtaaac catccaaagc ttctgcaaca gctttatttg taagttctaa tgcatctttt
    16981 atagtttttc cttttatcat ttcagttgcc atagaagaac ttgctatagc tgaaccacag
    17041 ccaaatgttt taaacttaac atcctttatt acatctccat ctatgtctaa aaatatcttc
    17101 attatatctc cacatgtagg gtttccaact tcacctacac cacttgcatt atcaatttct
    17161 cccatatttc ttggattcat aaaatgttcc ataactttat cactatattg cattatcttt
    17221 tcctcctact caatttatgt ctatttttta tgctttatct ttctgtctat tttactaaac
    17281 cttgtttctt cgcatcatca taaagtggtg acatgcttct aagtctttct attacttttg
    17341 gtaagttctc tagtatataa tctatatcat catctgttgt aaaatctcca actgtaagtc
    17401 ttaatgaacc atgtgctatt tcatgtggta aacctattgc aagaagtaca tgtgaagggt
    17461 ctaatgaacc agaagtacat gcagaaccac ttgaaccagc aattcctaac atatctaata
    17521 ataaaagtat tccttcacct tctataaatt ggaatgcaaa gtttgcattt cctggcaatc
    17581 tattttctaa gctaccattt actcttgtgt aaggtattct ttctaacaca ccatctatta
    17641 acttatttct aagacgagtt aaagtttcta catgattttg catattttcc ttagctaatt
    17701 ctgctgcttt tccatatcca actattgcag gtatattttc tgtaccagct cttttgcttt
    17761 tttcttgtgc tccaccatgg acaaagttat gaagtcttac accttttctt atgtataaag
    17821 ctcctacacc ctttggtcca tatattttat gtgaagacat actcatcaaa tctattccta
    17881 attctttaac atctactgga atattacctg tagcttgtac tgcatcagtg tggaataata
    17941 tcttatgttt tttagctatt tcggatattt ctttaatagg ctgtatagtt cctatttcat
    18001 tatttgcaaa cattatgctt ataagtatag tagtatcttt tattgaatct tctagttgtc
    18061 ttaagtctac tcttccttcg ctatcaacat ctaaataagt tatttcaaat ccatgatgtt
    18121 ttgctaagta ttcacatgta tgaagtattg catggtgctc tatttttgaa gttattatat
    18181 gattaccttt gtttttatta gcataagcta caccctctag tgtccagtta tcactttctg
    18241 aaccacctgc tgtaaaatat atttcactag gttcagcatt tataagggct gctacttgtt
    18301 ctcttgcttt atccaaagca tcttttgctt ctcttccgaa tgtgtgaaaa ctagatgcat
    18361 taccaaaata atctgttagg tatggcatca ttgcatccaa tacttctttt ttaataggtg
    18421 ttgttgcaga ataatccata tacaatcttc ttttttccat tacattcatc tccaaatcta
    18481 ttttttaata tttgttatat cattttctaa tttatttttg ttatagtcat ttatcatatc
    18541 tttaagagta attgaatcta taacatcttc tatacccttt ttcatctttt cccaaactat
    18601 ttttgttaca cacatgtttg agttttcaca tacatcttca tctagtacac agtcagataa
    18661 tgcaactggt ccttctaata ctacaagtat atctcctact gttatgtctt cagcattctt
    18721 tgaaagcaaa taacctcctt gagcacctct cacacttttt actagacccg atttttttaa
    18781 agatgaaaat atttgttcta agtactgatc tgatattttt tgcttcttag caataaattt
    18841 taatgatact ggtccattgt cttgatttaa tgctaattca aacattgctt ttagtccata
    18901 tctaccttta gtagacaact tcatatatta tcacctctat atagaaatct aattcatact
    18961 gttttagtag gaattacttt caagataatt ttattatacc ctactaattt tgtcaagatt
    19021 aattttaatt ttttattttg aataaattcc ccattttcca atgttttcaa atcctagttt
    19081 tttatatatc tttccagcct cttcattatc ataaaacaaa caaggaattt tgttttctct
    19141 cagtaactct gaacatagtt taattaggca ttttgtagca agtcctttag ctctatattt
    19201 aggatgtgtt cctactccaa ttatcattgc atgagtccta ttttctgaag tactctttgc
    19261 cattgacacc acctgtttgt taatctctat acaatatcct ctacctgtcc ttagtccatt
    19321 ttttaaattc tctaaagttg tactgtgaaa ttcatctatt aagtcataaa gtttcactgt
    19381 cttttttaga tttccaagcc taattctctt aacctttgca ttacaattat tatctatcaa
    19441 aaacttttta gtttgaagtt tgcaaaaatc aactaccttt aaattattaa gtcctaatct
    19501 ctttgctaaa gtatctacag cacatatttt cccagatatt tcttcgtaat tagtattacg
    19561 aagtagatta taaaaacctt caacatcata ctctccatca gaataaaaca tcataaattc
    19621 aaaatattta agtaatattg cctttatttc accatgttca cccacgtctg cccatatatt
    19681 taaaaaatta tttccatatc catatctttc aatatcccct attataaaca aattgtactc
    19741 aggttctttt tttaagtatt tcataactct actgtgataa gttgagttaa gttttataat
    19801 cataatatct acccccgata cataaaattc agtctaaaaa tcactattta gatattctaa
    19861 atattttttt atatttttct cagttttatt ttcttgtggt atgtaataaa tactatcttt
    19921 tatgctatct ggtaaatact gttgctttac ataatgattt ttaaaattat gaggatattt
    19981 gtatccacaa cccctaccta tttctttaga accagaatag tgagcatcct ttatactatt
    20041 tggtatatcc ttaacaaact ctttatccag ttcatccata gctttcatta ttgctataca
    20101 tgcagaatta gactttggag atgtagccaa aagaatagta gcttctgcta atggtatttt
    20161 cgcttctgga aatccaagtt gcatagctga gtctacacat gcctttacaa ttacaatagc
    20221 atttggataa gctaaaccta tatcttcact agctattact aaaagtctcc tacatatact
    20281 tatcaaatcc ccacctttta taagtcttgc tagatagtgt atagatgcct gtgggtcact
    20341 tcctcttata gatttttgaa atgcacttaa aatatcataa tgactatcac catttttgtc
    20401 atagtttata attttattaa atgtagatgc ccttattaca tctgaatcta tataagttat
    20461 attatcctta tttggtttag ttgaatagac taccacttcc aaaatattta aggctcttct
    20521 catatctcca tcagaaagaa ttgctatatc ttcaatagca ttattattgc actcaatatc
    20581 catatagcta tcttcattta atacttcaac agctcttttc agtccttttt ctatatcaag
    20641 tttttctaaa ggtttaaatt caaatacagt agacctactt aaaagagctt tatatacata
    20701 atgatatgga ttttctgtag tgctagcaat caaagttata ctcccatttt ccataaattc
    20761 taatattgat tgttgctgtt ttttattaaa actttgtatt tcatctatat ataataggac
    20821 tccatttaca ttatttatac ttccaagctc tgatatcacc ttttttatat cttctaaaga
    20881 tgaattagta gcatttattt tatagaagct tttattgctt ctttctgcta ttatttccgc
    20941 tactgtagtt ttaccaacac caggaggacc aaaaaatatc atatttggta aaaaattaga
    21001 ttgtaatatc ttagacaata tttttccatt acctattata tgactttgac ctattacatc
    21061 attcattgtt tttggtctta ttttatctgc taaaggcatt ttctcactcc tttttaatat
    21121 acttatcatt accttaataa ttcgtctaaa aaaggtatct atttgaaata gatacctttt
    21181 ctaatctaaa cctataaaat atagattatt gattgttttt taattttttt aattcatctg
    21241 taagtttgtc attaagtatt cttatatgag tacctttcat acctaatgat cttgattcta
    21301 tcacacctgc actctcaaat tttctaagtg catttactat tacagaccta gttataccaa
    21361 ctctatcagc tatcttactt gctacaagta gaccttcttt tccatccaat tcagcaaaaa
    21421 tatgttcaac tgcttcaagc tcggagtagg acagagtgcc tattgccatt tgaactacag
    21481 ctttctttct catttcttct tctaattctt cacctattgc tcttaatatt tcaagaccaa
    21541 caacagttgc actgtattca gctattacta aatcatcatc attgaatgaa tttgaatatc
    21601 ttgaaagtac caatgttcct aatctttgac cgcttcctaa tattggaact actgtagtat
    21661 atttttgtaa tcttccatgt tcttcaggaa agatttctaa tatcttctca ccatttaaat
    21721 tttctaatgt ttcatcaatt tttaacacat tttgagtata ttcatctgaa aatttctttt
    21781 gcttagtata ctcatcttct ataactgaac tgtcttgaac atcatttaaa tgaagaccta
    21841 gtactttacc ttttgcactt actacataaa cattagaact taaaacgtcg cccaatgctc
    21901 cggccagtaa atcaaaagag acactgcttc caccacttgt ttgtaatgtt ttatttattt
    21961 tccttgtttt ttgtaacact tcacttgcca tttaatcatc tcctcatgaa atttaggcct
    22021 tttaataacc ttttatattt tctaatatta ttttcacatt taatgaaaat ttatttttct
    22081 attagttgtt tacacttgaa agattatcat agattggaac ctttttcaag agaattatgt
    22141 gatttttttc aattttcact cttttccgtc attatgctct tttgtctctt tattacattc
    22201 tttattagaa cacactgttt ttgtttcaga tttagtcact ttttcaacca tatatgaacc
    22261 acacttctca caaaactctc ctgttggctt attccaagct acaaagtcac agtttggata
    22321 attagaacac ccataaaaag ctttacctct tttagatttt ctaagtataa tatctccttc
    22381 ttcacatttt ggacatttaa caccaatttt attcataatt ggtctagttg ttttacaatc
    22441 gggatagttt ttacaagcca taaactttcc aaatctacca tatttgataa ccatattaga
    22501 cccacagttc tcgcaaatct catcagtttc ttcatccata tttacttttt ctatattttc
    22561 aagagcttct tcaatagcct ctttaagtgg tgcatatgat tctgatacaa tctttttcca
    22621 atctgtattg ccttcttcta taacatcaag tttactttcc atatcagcag taaaatctac
    22681 atctataaat ttatggaaat tattttccaa aatatcagta actattattc caagttctgt
    22741 tggacataga cttgttcctt gcttttcaac atactctcta ttcagtatag tagctattgt
    22801 tggagcatac gtacttggtc tacctatacc tagttcttct agtgttttta ctaaactagc
    22861 ttctgtatat cttgctggtg gttgtgtaaa atgttggtct ggcaatatat tttctatatt
    22921 taatatatct cctttttcta tacttggtaa aattttgtct tctctttctg aaaaattata
    22981 taccttggta taaccatcaa atttcatttt tgaaccagtt gccttaaata caatatcgcc
    23041 tattttacat tctacattta gtatatcaaa cactgaatct tccatttgac tagctacaaa
    23101 tcttctccaa attaaattgt ataacttgta ctggtctttg ctcaatgagg cttttatact
    23161 ctctggagtt ctatcaactg aggttggtct tattgcctca tgggcatctt gaaccttttt
    23221 agtatccttt ttttcttttt tatcaccagt gtttttatag tagttttctc ctaaattgtt
    23281 tacgatataa cccttagctt tttcttttgc ttcatcagat attcttttag agtcagttct
    23341 tatatatgat ataagaccaa cagtaccttc accttctatg tcgatacctt catataattc
    23401 ttgtgctatc atcattgttt ttttagtagt aaaaaataac ttatttgctc cttcttgttg
    23461 aagcatactt gttgtaaatg gttttggagc tgatttacgt cttgatttag attctatctt
    23521 ctcaaccttt aaatctttac cttccatttc ttttagtatt tcatttacag cttcttcatt
    23581 ttctaattca atcttttcat catttttacc ataaaatttc aaagttatat ctttgtcttt
    23641 tactgtctta gcctcaacat ctattgtcca gtattctttt ggaataaatg cctttatttc
    23701 tttttctcta tcacaaatta actttgttgt aacagattgc actctacctg cactaagacc
    23761 ttttctaacc ttttgccata atataggact tatttgataa cctaaaagtc tgtctagcac
    23821 tcttcttgcc tgttgtgcat caaccaaatt tatatcaata tttctaggat gtttaattgc
    23881 ttttttaatt gcatccttag ttatctcatt aaactctatt ctacaatcct cagattcatc
    23941 taaatttagt atatgagcta aatgccatga tatagcttca ccttctctat cagggtcagt
    24001 tgctagatat acttgtttag attttttagc ttcttttttt aattctttta ttacatcgcc
    24061 ttttcctctt atatttatat attgaggttc aaaattattt tctatatcta cacctaattt
    24121 acttttaggt aaatctctga catgaccaac tgatgcttta actgtgtaat gacttttccc
    24181 taaaaatttt tctatagttt tagcttttgc aggcgattct acgatgacta atgttttggc
    24241 cataaaacca tacaccccca ctacctaatc ttaatttata catttaaact gtacgtttta
    24301 ttattcattt ctactactaa ctcatttagt actaattcat tgattattga atttacatac
    24361 tttatttcta taccagtata atcacaaata ctatctatat gcaagtttcc ttctcttttt
    24421 atagcttcaa ttatttgctt agacttggca tttagactta tattatcata tttttgattt
    24481 atttttttat caataatatt aaatatgtta tattcattta aaatatcatc tatattttca
    24541 attaattttg caccttcttt tataatttta tgacaacctc tactcatttg agaatttata
    24601 ttaccaggta ttgcaaaaac attttttcca tgttcaagag caaaatctac tgtgattagt
    24661 gcaccacttt ttattgctgc ttctacaaca actactccat cacttaaacc actgataatt
    24721 ctatttctat tagaaaaatg gtacggggct actgtcgagt taatattata ctctgataat
    24781 aataatcccc cattttctaa tattttattt gataaatgta aattttgttt tggaagagga
    24841 ttgtctactc ctgaaccaag tactgctata gttttagatt ttccactcat acaacctatg
    24901 tgcgagtatg catctattcc tatagctaat ccacttacta tattaactcc atattgggat
    24961 agttgacaac ttattgtttt tgcacaatta attccatagg ctgtaggttt tcttgaacct
    25021 actatagcta tattaaaatt attattaact aaacctatgt cacctttgta gaataataac
    25081 tttggagcgt tgtatatatt ctttaaattt tcaggatatt ctttatcata tttacaaata
    25141 tatttaatcg cttttttgta aagtagttct tttatatttt ctaaataagc gtaacctcta
    25201 tattttacta tattttttct aatattcaag cttatatttt ttaaattata tatttctttt
    25261 tcagaaaaat caaatatatc ttcaatattt actatttcat tttctattat ttcaattgtt
    25321 tttgttgtta ttccaccaat tgattttaac cataaataag tatctctttt ttccataaaa
    25381 tccacctcaa aaaactattt aaaataacta taataagcct ttctaaatga aaaagcttct
    25441 ataatacact gtgaatttat caagtctctt tcttctaaat ctgctattgt tcttgccatt
    25501 tttaaaagtt ttgtataact tctactgctt aggttgtatt tattaaatat catctcagcc
    25561 gtttttgaag cttctttttc cagtttacaa tatttaaata aattgtaagc tttaatttca
    25621 ctattatttt ttatattatc tttcttaaat ctatttatct gtatttttct agcattttct
    25681 actcttctct ttattttttg agagctttct tcttgtttta aagaattaaa atctgaatat
    25741 tttatagaat taacttctac aaaaatatca aacctatcta acaatggacc tgatatttta
    25801 tttatgtatc tatcaatttc gttacttcta catctacact ctgtctctga catataataa
    25861 ccacatggac atggattcat agctgcaact aacagtactc tacaaggata ttttgcactg
    25921 tactttactc tggatatatt tacatatcca tcttctatag gttgtcttaa agtctcaagt
    25981 atttttctat caaattcggc tatctcatct aaaaataaga tacctctatg tgctaaagca
    26041 atttctccag gtcttgcatc cattcctcct ccaattaaag attgctttgt agttgtatga
    26101 tgaggtgctc taaaaggtct tttatcaatt attcctttac tttcatttat catacccaat
    26161 atactataaa ccttacttac ttctatcatt tcttctatac ttatatctgg taaaattgtc
    26221 cttactcttt ttgcaatcat agtttttcct gagcctggag gtccaatcat aaacatatta
    26281 tgatttccag ctgctgcaat ttctgcactc ctctttacaa aataatttcc ttttacatct
    26341 ttaaagtctt catcatattt actttcttca ttattaatct tagtatattt gcaatctata
    26401 taactattat ctttttctaa ttctccgttc tcttttcttg acttatcatc caaaaaagac
    26461 attatactta ctttatcgac ttttatctct tcgtttaaaa aatcgacaca ttcttttaaa
    26521 cttttaatag gaattatatc aatttcatct actaatagac tttcttttat gttctcaatt
    26581 ggtataaaaa tacgttttat gttctgagtt tttgcaccca taattatggg caatatccct
    26641 cttacttttc ttatttttcc atctaatgat aattctccta taaacatact ttctcttatg
    26701 taattttcat ctttttttat taactctctt aaaaggccta tacttataga taaatctaaa
    26761 aaagctcctt ctttttttat atctgctggt gataaattta caactattct cgtgcttgga
    26821 aatttatatc cactattaag aattgctgac tttactcttt ctcttgcttc tttaatttct
    26881 ttgcctggaa gaccaacaat attaaaacta ggtattccat tgcttacatc tacttcaacc
    26941 ttaactaaaa agctgtcgat tcctactaag ttgcttgaat taataatact taacatattt
    27001 aatcctttcc aaaaacatta tcttttttaa aaagcattca ttatgtgatt tattcttttt
    27061 tctaacaaat agacctctat gatgtcaaat cttatgggag tattatttaa gttatttttt
    27121 ataatatagt attttgcgac accaattatc ttttttcttt ttttaaaatc tacagattca
    27181 caagggtagc catactttat gttagacctt gatttcacct ccacaaatac aacttcatct
    27241 cctatatttg ctattatatc tacctcccct atccttaatc tataattttt ttctagtatc
    27301 tttgcacctt ttgatagcag ataattaact gctatttctt ctccaaaatc acctttttct
    27361 ttattattca ttatctactc caatatatta aatttttact tttaaattat cgtcagtata
    27421 caatttaact attcaatttt ttgaaattga ttttatttcc attgatatta ataaatactt
    27481 ctattgactt tattctttta tgatattatg ctagttttta taatgaaagt ttatttttat
    27541 ggaggaatgt ctttaatgaa cagaaaaaaa acatttattt ttatacgata tttatctcta
    27601 tttatattac ttataagtta tttaaaccat actgaaaata cattgtatcc agctataaca
    27661 atgattatat tatttctaat aataaataat caagttagat ttttttcttt atcaagtgag
    27721 aataaattta tacttatttc ttatttttta gagcttattc ta
//
LOCUS       DAMJWP010000026        27368 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.027, whole genome shotgun sequence.
ACCESSION   DAMJWP010000026 DAMJWP010000000
VERSION     DAMJWP010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27368
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.027"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            256..789
                     /gene="pyrR"
                     /locus_tag="PC700_001009"
     CDS             256..789
                     /gene="pyrR"
                     /locus_tag="PC700_001009"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDF2580329.1"
                     /translation="MVEKAQLMDEKAIARAITRISHEIIERNKGVENLVLVGIKTRGV
                     PIANRISKKIEQIEGTKVDTGDIDITLYRDDLEKIHVEPVVKGTYLDFNVNDKTVVLV
                     DDVLYTGRTVRASLDAIIDIGRPKSIQLAVLVDRGHRELPIRADYVGKNVPTSRHEII
                     SVSLLEIDGEDSVTIKE"
     gene            1073..2284
                     /locus_tag="PC700_001010"
     CDS             1073..2284
                     /locus_tag="PC700_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDF2580330.1"
                     /translation="MKKTIMSLQHLLAMFGATVLVPILTGFNPSVAIFCAGVGTLIFH
                     FCTEGKVPAFLGSSFAFIPVILAAKEAYGGDLAYAQGGIIVAGLIYIIMSIIVKIVGV
                     NKIKLYFPAQVTGAMIVVIGLNLLPTAFSMASANFVIAFITLAIAILTNKFGRGFIKQ
                     LGILIAVFSGYIICLLLRLVDITTITEASLFAIPNFTVPKFSLGAIVIISPVVLAVFM
                     EHIGDMTTNGAVVGKNFIENPGLNRTLLGDGFATIVAGCLGGPANTTYGENTAVLAIT
                     KNYDPSILRRTAIFAILLACVGKFGGFLQSIPGSVMGGISIMLFSMITYVGLKTIRDS
                     SCVESKINILIIAVILLIGLGTTYLSNKGISIGIPITSTVKITGLSLAAIVGIVLNRI
                     LNNQDFKVEEE"
     gene            2583..4235
                     /locus_tag="PC700_001011"
     CDS             2583..4235
                     /locus_tag="PC700_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF2580331.1"
                     /translation="MLLVKVENLKKYYADKLILDIDKLEILENDKIGLVGSNGQGKTT
                     LLKAILGEIEIDEGYTYLTESYSYISQSENNIETCSHSKEKSLLNAPDKFEEYLSGGE
                     KVKLKIADALSNKKNIIIADEPTSNLDKKSIGLLEDMFKRHEGALLLISHDRRFLDEL
                     CTTILELEDGKLKAYKGNYTDYLMQKDEEVKRADFEYQEYVKEKKRLEKALLYKKALS
                     DGIRKTPKRMGNSEARLHKMGGQTNKKKLDSNVKAIKSRIDKLEVKNKPKVSKEMNIK
                     IQDGMEIISKNLLEVKDMTLKLENKLLLDNVSFKIKRGKKIALLGDNGCGKSTLIKEI
                     LADKNDNIKINNKVKVGCFDQNQSLLDEEKSVLYNTKVNSSFDESFIRINLSLFGFKG
                     DDVYKKVKVLSGGEKVKIALCKIILEDNNFLVFDEPTNYLDIKSMEALEKALINTDKT
                     MLIVSHDRVFVSHICNYIIEIKDAKIREFDCNYDEYIISRNKKTPSRDKQIKKENLLV
                     LENRLTTVISMLSIEKDNLKKELYESEYNELLKQITKLKNSF"
     gene            complement(4450..6225)
                     /locus_tag="PC700_001012"
     CDS             complement(4450..6225)
                     /locus_tag="PC700_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HDF2580332.1"
                     /translation="MALDGLVIHSIVDELSSKLTGGKIDKIHQPEDDEVIFNIRNNKE
                     NFRLVLSASASNPRVYLTSNYQKENPLKAPMFCMLLRKYIQGGNIVEISQIGFERIIK
                     ISVESLDELKEKTVKNIMIEIMGRHSNIIITHGEENKIIDSIKRVPFSISRVRQVLPG
                     HDYSLPPEQNKLNPLDDISKDLFIKNLEELEGPIFKSIYSKFLGISPIIAKEICYRAG
                     VNQNAIIKDISDEQFDSLHKVFCNLFNDINSNKYSPCIIIDKKVDKVVDFSCINLTLF
                     SDLSYINKDSMSRILEDFYRTKDIKDRINQRSSDLKKSISVKLDRLYNKLKKQEEELS
                     ESENADIYKIKGELITSYIYMVEKGMESIEIANFYDENCNDVTIELNKNLTPSENAQK
                     YFKKYNKMKHAKVEISHQISLNKEEIDYLENIVLSIENCENLAELQDIKEELAKVGYI
                     KTQKKNSKKDTIPSTKPHEFVSSDGFKILVGKNNKQNDYLTLRLADNDDLWMHTKNIP
                     GSHVIIKCAGKEVPDNTVFEGAMLAAFFSKSKLSSQVPVDYTKRKNVKKPSGSKPGMV
                     IYETNSTIYVTPEEETVAKLKVKSE"
     gene            6521..7555
                     /gene="ltaE"
                     /locus_tag="PC700_001013"
     CDS             6521..7555
                     /gene="ltaE"
                     /locus_tag="PC700_001013"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HDF2580333.1"
                     /translation="MRFIDLRSDTVTMPTDEMREAMAKAPVGDSIFRDDPTVNKLEEL
                     AAAKVGKEDAIFLPSGTFGNQLALFTHCLRGQEIIIGKGYHIVTHEVGAPAVIAGVQL
                     RTIDEDESGALNPELVEKAIRRDDIHEPQTGLICVENAYSGGTVVSLDNLREIKKIAE
                     KHNLPVHLDGARLFNAALTLGVEAKEIAKCCDSVMFCVSKGLAAPMGSILAGSKEFIA
                     KARRKQKVMGGGMRQVGIVAAAGIIAIEKMTLRLNEDHENAKYLACELNKIDGIEVNN
                     VNPDISMVFFKMSEDIIKEDVLIKEFFERNIKINKMENGEYRFVTHVDITKEDLDYVL
                     KTLKELIGVC"
     gene            7842..8672
                     /locus_tag="PC700_001014"
     CDS             7842..8672
                     /locus_tag="PC700_001014"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDF2580334.1"
                     /translation="MKFWKLHGIGNDFIAIDGRFDQINPSDYTALAKKVCHRRFSVGA
                     DGLLVVKDSKVADVEMVYYNSDGSRAAMCGNGLRCFCKFVYDNCIVKKESFTVDTLDG
                     IKDIKINLTNREINSIRVNMGKGSFIAKDVPVLTNKERFVQESIKVLDRELKVTSMLM
                     GVPHTVVIVDELDVDDVCKYGKEIENNKIFPEKTNVNFVKVEDKYNIHVYTWERGCGY
                     TLGCGTGMTASAIVCNLLNMVESSVNVTSQGGTVKIDVGDFSYMTGPAVKICEGVLEV
                     "
     gene            8676..9557
                     /locus_tag="PC700_001015"
     CDS             8676..9557
                     /locus_tag="PC700_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDF2580335.1"
                     /translation="MAISMTGFGRGEYKDDNYYFLVECKTINHKYSDINIRLPRKISF
                     LEDKVRNLVKNYVKRGRVDLYIKFDLLGKEDVNLNFDEGLASQYIDILKEIKNKFDII
                     DDISVMNVAKFPDIVKIEEKEEDEDLLWSMLNQAVEDALIKLREMRSEEGKKLAEDIA
                     MRCDLLKNHIEEIEKYSSSVVEDYREKLNLRISELLDDPSIIDENRLAQEVAIYADKS
                     SITEEIVRFKSHIGQLKNTIFKDDSIGRKIDFLIQEMNRETNTIGSKSSDINITNLVV
                     EVKSELEKIREQIQNIE"
     gene            9587..10204
                     /gene="gmk"
                     /locus_tag="PC700_001016"
     CDS             9587..10204
                     /gene="gmk"
                     /locus_tag="PC700_001016"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDF2580336.1"
                     /translation="MFVKKGLLLVVSGPSGAGKGTICKELLKENDTIKLSVSATTRKP
                     RTGEVDGVNYFFISKEKFEEMIEKGEFLEYAQIYDNFYGTPKAAIMECLEKGQDVLLE
                     IEMQGAKQIKEVCPEGVFIFVLPPSLEELKNRIVGRGTETEAEIEKRFSCAYEEIKMI
                     KDYDYFIFNEDVKTSAKEIEGIISSEKNKVSRYKNIIIEKFKEEL"
     gene            10207..10473
                     /locus_tag="PC700_001017"
     CDS             10207..10473
                     /locus_tag="PC700_001017"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDF2580337.1"
                     /translation="MLKPSINEVLEKIDNRYYLVGTVSKRARKLIDGEEPYVSNKTKE
                     KPVCVATKEVASGKITYRLLTEEEIEIEEARHHAEQHQQISEEE"
     gene            10478..11677
                     /gene="coaBC"
                     /locus_tag="PC700_001018"
     CDS             10478..11677
                     /gene="coaBC"
                     /locus_tag="PC700_001018"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HDF2580338.1"
                     /translation="MLKDKTVVIGISGGIAAYKACDVVSKLKKLNANIHVIMTKSATE
                     FVKPLAFQSLSQNYVVEDMFEEPKTWEVEHISLAKKADLFLIVPASANVIGKIANGIA
                     DDMLTTTVMATKAQVLIAPAMNTNMYENPIVQKNIQTLKSFGYKFIEPESGRLACGDI
                     GTGKLANPDIIVEEVLTNLTKEQDLKGQSMIVTAGPTVESIDPVRYLTNRSSGKMGYS
                     IAREAIARGADVTLISGPTNLSIPQNLKKFVQIESAQDLYEAVVSNLSENKIVIQSAA
                     VADYKPKTYSDKKIKKKNDDLSIELSRNYDIAYEIGKIKEDKILIGFAAETNDLIEHA
                     KGKIKKKNLDFIVANDLTKEGAGFRADTNIVKIIDVEGNITEYPKMKKEEVANVILDK
                     VKALLEK"
     gene            11916..14441
                     /gene="priA"
                     /locus_tag="PC700_001019"
     CDS             11916..14441
                     /gene="priA"
                     /locus_tag="PC700_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDF2580339.1"
                     /translation="MNNILNLSRGFIMKKYAKVIVRSNTIYTDNLFTYQIPVFLSDVI
                     KIGHRILVPFGKGNKPTEAFVFQFTDSLDEKIKIKEIIDILDENPIFRKEDLELVYWM
                     KNRYLCTYIECINLIYPKGYKLNNYKVVLLGESLSGLNEIELKEKISTLSDKNREIVE
                     KVIDSKGKIKVDKLKYIPNLNSSLYTMNKNGIIKLCWEYKNHKNEKKVCYISLSLESD
                     KIDDYIEQNKINVGSKQKEILSFLKNNENVEINDLLDLLNASKQSINSLSKKKLITLE
                     FKDYYREPKSIYKSVLKSIKLNNEQQEAVDEIKSNMFVDDKKPYLIHGVTGSGKTEVY
                     MEIIEFALNQGLDSIFLVPEIALTPQTIDRLKSRFGDLVGVFHSKLSEGEKHDVYKAV
                     KAGKVRVLIGARSALFAPFNSLGLIIIDECHESSYKSEKNPKFNAIEVARFMALKNNI
                     TLILGSATPSIEEYYRAKSGEYKLINIKSRANDKPLPNIEVVDMKDELDKGNRSIFSL
                     KLQKEIRYAIEENNQVILFLNRRGYANFVSCRKCGYVFQCENCDISLTYHKKSNTGRC
                     HYCGYEKEIPKECPECKSTYVKPFGVGTQKIEEELKYIFPDIKTLRMDKDTTSKKGAL
                     DEILNKFKDKEADVLIGTQMLSKGLDFENVTLVGILSADMILNFPDFKSFETTFQLIT
                     QVSGRAGRADKEGKVVLQTYDTEHYAIKHAIEYDYEGFYEDEIKIRKAFGYSPFNNML
                     SVVVSGEDERLVIKNIKNMHASLIYLLEKRGINDLGFILGPNPCSISKINQNYRWQIL
                     FKDENIEINLLKGIIKYICITKRDLIFDKNINVSIDINPNSVL"
     gene            14459..14899
                     /gene="def"
                     /locus_tag="PC700_001020"
     CDS             14459..14899
                     /gene="def"
                     /locus_tag="PC700_001020"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDF2580340.1"
                     /translation="MALRQIVQIGEPVLRKKSKKVEKIDEKIIQLLDDMAETMYDADG
                     VGLAAPQVGILKRVVVIDIGEELIELINPEIIETSGEQIDEEGCLSVVGEAGNVRRPN
                     YVKVRALNRNGETIELEGEELLARAFCHEIDHLDGILFVDKIEK"
     gene            14914..15843
                     /locus_tag="PC700_001021"
     CDS             14914..15843
                     /locus_tag="PC700_001021"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDF2580341.1"
                     /translation="MKIVFMGTPDIAVPCLQKIIDEKYEILGVVTQPDKPKGRGKKLG
                     MSPVKELAIENNIPVYQPVKARDKEFIDKIKSLNPDVIVVVAFGQILPKEILEIPKLG
                     CINVHVSLLPKYRGAAPINWVIINGEEKTGVTTMYMDEGLDTGDMILKTEVNLDENIT
                     AGELHDKMMNIGAETLKETLRLIEEGNAPREVQNHEEFSYAPIMNKSLGNIDFSKSAR
                     EIHNLVRGVNPWPSAYTIYNDVIMKVWKTKVLDEKSTKDVGTIIDVSKDGIKVSTIDN
                     VLLIEEIQMPNKKRMLVGEYIKGNTIETGLVLG"
     gene            15853..16605
                     /locus_tag="PC700_001022"
     CDS             15853..16605
                     /locus_tag="PC700_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HDF2580342.1"
                     /translation="MTDIKKYLTSVGILILILGILIGVVNFFIISLTQIFSLMINIII
                     ISLILIILCSTAVTYRVIKGKYVNSNIMKINFNIVSGLFPIISFIASSFGMSKSDIRR
                     IYIKLNNEYIYSNKYNFNPEDIIILIPHCIQENSCKLKVTNDIDNCKECGRCNVGELI
                     KLKEKTNVKIFVATGGTLARKIIMDNKPKAVVAVACERDLTSGIQDIKKIPVLGVFNK
                     RPNGPCVNTNVDMMDIEKAIDFLTGKNILICN"
     gene            16674..17375
                     /locus_tag="PC700_001023"
     CDS             16674..17375
                     /locus_tag="PC700_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HDF2580343.1"
                     /translation="MYPVYGGFWGFDPTMVVLIPAILLTIYAQFKVSSTTNKYLRVNT
                     RRGYTGEQTARRVLDSNGLYDVKIEMVRGHLSDHYDPRRKAVRLSEDVYYGTSITSVA
                     VAAHECGHAIQHAKGYAPLQIRSSLVPVVNFASSISWFLIFLGFIMAGPFLKIGILLF
                     SASVLFQIITLPVEFNASSRAIVQLGNLGIIDESESRQSRRVLSAAALTYVAAALVSI
                     LQLLRLLLIAQRRND"
     gene            17437..18762
                     /gene="rsmB"
                     /locus_tag="PC700_001024"
     CDS             17437..18762
                     /gene="rsmB"
                     /locus_tag="PC700_001024"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDF2580344.1"
                     /translation="MDAREVGFKVLCDIEKNDNYSNIAINKHFKNLEISDMDRGLATE
                     LIYGVIENKYYLDYIINKLSKIKVKKMSTYVKIFLRMGTYQILFLDSISDYAAVNETV
                     KLSKKYDKKSSGFINAILRNEIRAKETIMDITEEDSVKYLSIKYSYNSWIIKNWIDKF
                     GQEFAEDLLEANNEKPSIYIRTNTLKISREELIEKLSEMGIVCLKVPMVEEAIKVEKM
                     KNIEKNELFKAGLFTIQDISSMIVGKVINPKEGSFVLDVCSAPGGKSTHLATLMNNTG
                     QVVARDIFEHKLKLINSTVNRLGLKNVFVERFDASEIDENSISKFDYVLADVPCSGFG
                     IIRRKPEIKYKKEEELKDITSIQKKILENASKYVKVGGTLVYSTCTIQDVENINIVTS
                     FVEENHNFEFVPIDTVNVDLDNQDKGYLKIYPNIHGIDGFFIAKLKRIR"
     gene            18770..19801
                     /gene="rlmN"
                     /locus_tag="PC700_001025"
     CDS             18770..19801
                     /gene="rlmN"
                     /locus_tag="PC700_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDF2580345.1"
                     /translation="MEKKKIVLKNFTEDELKEFMKTIDEKPFRGSQIFSWIYKGAKTF
                     DDMNNIPKSLRNKLEEVSCIGNIDIELKLESKVDNTKKYLFLLDDGNIIETVMMDYDS
                     RVTVCVSNQVGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGS
                     GEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLH
                     SPYDEERRKIMPVANAYSIEEILNACRYYIKKTNRRVTFEYSLIKGVNDSEKEAKALA
                     KLLKGMLCHVNLIPINKVEEREYEKPDKAFIYKFRDSLEKNNIPATVRMSMGSDISGA
                     CGQLRRKYK"
     gene            19821..20573
                     /locus_tag="PC700_001026"
     CDS             19821..20573
                     /locus_tag="PC700_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HDF2580346.1"
                     /translation="MVYSCASHIGKIRKNNEDYCEGEVIDTEHGPIGIFAIADGMGGH
                     KKGEVASKLAVENIIDFLKENLLQHDNVKIDYIDDILKQAYNNVNSIVHKKSMEDIEF
                     EGMGTTLVTAIVYNNVLYVANVGDSRCYLLTDEKFDKITIDHSVVEELMMAHVITEEE
                     ARRHPQRNRITRAIGTDDMVVVDIFKKEIKKSDIILLATDGLTGFIDDEDIRDLILDY
                     EYERTSNISEELISMANDVSGKDNVSVIVIKV"
     gene            20590..22605
                     /gene="pknB"
                     /locus_tag="PC700_001027"
     CDS             20590..22605
                     /gene="pknB"
                     /locus_tag="PC700_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HDF2580347.1"
                     /translation="MGDTILGNRYEIIRKIGDGGMAFVYEAKDRLLNRTVALKVLRPE
                     FVDDDEFLTKFKREAEAVASLSHPNIVNVYDVGEDGKVHYIVMEFVDGKNLKEIIQDE
                     GILDEYTALDITKQIAMALSAAHKKGIIHRDIKPHNILISNEGRVVKVADFGIAKAVS
                     NSTMTNIGSIIGSVHYFSPEQAKGKFVTNNADLYSLGIVLYEMLIGKVPFRGDSPISI
                     ALQHINDDIDFTSEEKVRIPQSVRTTIKKLTEKSSADRYQTAEELIEDIEYIEKNIDL
                     DFIKEYDDFATKKIDEKEINKVVNPTLAKPAPEKVVKPVEVADLDDDEDYYDDFYEED
                     EEEEEEEEEIMRAKKNQRPKSTPSKRTKKKKKKQESPKSRRRLKVIAAVLILILCAQV
                     FLAYKFLFAGGFGNKSLTVPNLVNMTLEEAQSAVEKEGLYLSVKSEEYNSEVDENCII
                     SQTPEGGSTNVKKGDTINVVVSKGSSQASVPNVVGLTLSNAKQLIEENNLKVGTVKYE
                     YSSIYKEGTVLSQSPGAGSSRAQEGDEVNLYVSKGSEKSNTQTPTVPDKKPTTPENNT
                     PTEPGSNSGNSGGNSGSSNSGNSGGNSGNNGGNSGGSNSGNSGGSSGGSNSGDNGGSS
                     GGSNSGDNGGSSGGSNSGDNGDSSGGSNSGDNGGTSSGTGANIGKTE"
     gene            22713..23450
                     /locus_tag="PC700_001028"
     CDS             22713..23450
                     /locus_tag="PC700_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDF2580348.1"
                     /translation="MKKKTVLITGGARGIGKAMSKAFAKEGYNVLVNFNKSENEAKEL
                     YTILNEKNFSVKLFKADISNREDVENMVDYCIKEFGGLDVLVNNAGVSQDKLFTDITD
                     EDWDNMMNINLKGSFYCSQVALKYMISEKKGNIINISSIWGISGASCEVHYSITKAGI
                     IGMTKALAKEVGPSNIRVNSIAPGVINTDMLSGYNEEDIDALVEETPLMRLGTPEDIA
                     NCAIFLASDKSNFITGQVISPNGGFVI"
     gene            23548..24432
                     /gene="rsgA"
                     /locus_tag="PC700_001029"
     CDS             23548..24432
                     /gene="rsgA"
                     /locus_tag="PC700_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDF2580349.1"
                     /translation="MLEGKIIKGISGFYYVDTYNGIYECKARGIFRKQKITPLVGDRV
                     KISIVDEDEKKGILEEIDSRDTELIRPPIANVDKALIVFAIKNPKPNLSLLDRFIVLA
                     EKENLETVIILTKADLDDNDTLETVKNIYELSGYKVIPVSNITKLNIDKVKEELKENV
                     VVFAGPSGVGKSSLLNEIDENFKLQTGVVSDKIKRGKHTTRHAELLKLEFGGMVADTP
                     GFSSLALEDIEEVELKDYFIEFDKFNDCKFGSKCIHENEPNCAIKEAVTNGEISKERY
                     DSYIQLLHEIRQNNSRRY"
     gene            24437..25108
                     /locus_tag="PC700_001030"
     CDS             24437..25108
                     /locus_tag="PC700_001030"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDF2580350.1"
                     /translation="MIKLAPSILSADFAKLLEDVRKVESAGCEYLHIDVMDGHFVPNI
                     TLGPLVVKSLKKENINMVFDAHLMIENPDQYIEEFVKAGCDIITVHQEACVHLHRTIQ
                     NIKSHGIKAGVVLNPATPVDTIKHVLPDLDMVLLMSVNPGFGGQSFIPCVLDKIKELK
                     AIIDSQGLNIDIEVDGGISPKNVAEVVQAGANVIVAGSAIFGSDDIQETVNLFRKNAS
                     LEELV"
     gene            25117..25758
                     /locus_tag="PC700_001031"
     CDS             25117..25758
                     /locus_tag="PC700_001031"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HDF2580351.1"
                     /translation="MKICIVLNGEIEDYEVTRDIIIKECYDCIICADGGANHTYKMEI
                     MPDYILGDLDSVEEEKINFYKNKGVKFEKFPSKKDETDTELCLFLAKTLKANHIDFFG
                     ALGGRIDHTLANIKLLYYLKEDGIYSRILSDKEEMYIVENEEISLYGNPGDTVSVTAI
                     KGDAKGVTLTGLEYPLDNYYMKYSVPIGISNVMLSNSCKIKVEQGCVLVVRNL"
     gene            25889..26518
                     /locus_tag="PC700_001032"
     CDS             25889..26518
                     /locus_tag="PC700_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDF2580352.1"
                     /translation="MVDSIIFDLDGTLWDSTEEVCKVWQDVLSKHKEIELSVTKDLFR
                     SLMGLSFEEIANRLFPNLSEEERMNLLNECSLKECDYLSENGAKLFDDIEDTLKLLSK
                     KYKLFIVSNCQAGYIESFLKAHKLEQYFIDFECPGNTGLHKGENNKLIIERNKLINPI
                     YVGDTQGDANSAKFVGIPFIYAKYGFGNVDVYDYFIDSFKDLLEHDILK"
     gene            26573..27229
                     /locus_tag="PC700_001033"
     CDS             26573..27229
                     /locus_tag="PC700_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2580353.1"
                     /translation="MELQDTIFKRQSVRKFKNQDVSDEDILKMIKAAGAAPSGKNIQN
                     WHFVVIKRRDLMEKIADVITKKQQEILVEMDKVSVDKANRFRKFVKNFTLFYLKAPVL
                     VLVFTKVYNPSGYYELELIDAPKETIDKLFIRNPGMQSLGASIENFTLSAIELGYGSC
                     WLTSQNYAADEIEAVLEAETGFEKGEYFLGAMLALGVPEDNLKSPSKKPVEEICTFIK
                     "
BASE COUNT        10903 a         2770 c         5098 g         8597 t
ORIGIN      
        1 agcttgaaaa atttgaaata taaatatata atttgagttg atagattaaa aaattatctt
       61 taaacgatac agagatatcg taagacaatt gctaaattac actgtatatt ttttatataa
      121 gagcttcttg tcttgtgatg agaagttttt tgttgtttaa attgaaattt taaataaaaa
      181 taaatttata aaaaattaaa agaaaggagt agatttgtca aatagatata ttatatcttt
      241 gacaataatt atgatatggt agaaaaagct caattaatgg atgaaaaggc aatagcaaga
      301 gcaattacta gaataagtca tgaaataatt gaaagaaata aaggtgtaga aaatctagtt
      361 ttagttggaa taaaaactag aggtgtgcca attgcaaata gaatttctaa gaaaatagaa
      421 caaatagaag gaacaaaagt agatacagga gatattgata taactttata tagagatgat
      481 ttggaaaaaa ttcatgttga acctgtagtt aaaggaactt atttagattt taatgtaaat
      541 gataagacag tagttttggt tgatgatgtt ttatacactg gaagaactgt gagagcttca
      601 ctagatgcga ttatagatat aggaagacct aaatcaatac aattagcagt tctagtggac
      661 agagggcata gagagttgcc tataagagca gattatgtag gaaaaaatgt acctacttct
      721 agacatgaaa ttatatctgt cagtttatta gaaattgatg gagaagattc agttacaatc
      781 aaagaataat tagttttaag tattatataa aagcttagca ttgaatagtc agggaggagg
      841 ataaagctta catagattgc acaaaataaa tattataatt ttatggatgt gagtcttaat
      901 gtagttttta ttctccttta tttgagtttt tgatgtcgta aaatgtaaaa taatgataaa
      961 ttatataata agtatataga ggttaaaaaa tagaatctag ggaatgataa ttaattaaaa
     1021 gatattaatt aaacagagga tttatttata tataataatt ttggaggttt ttatgaaaaa
     1081 aacaataatg tctttacaac atctattagc catgtttgga gcgacagtat tagttcccat
     1141 tttgacaggt tttaatccgt cagttgcaat tttctgtgct ggtgttggta cattgatatt
     1201 tcatttttgt acggaaggaa aagtacctgc ctttttaggt tcaagttttg cgtttatacc
     1261 agttatacta gcagctaaag aggcgtatgg tggagattta gcatatgctc aaggtggtat
     1321 aatagtagca ggattaattt atattataat gtcgattata gtaaaaatag ttggtgtaaa
     1381 taaaattaaa ttatattttc ctgcacaagt tacaggagct atgatagtag ttataggatt
     1441 aaatttattg ccaacagctt ttagtatggc ttcagctaat tttgtaatag catttataac
     1501 tttagcaata gcaattttaa ccaataagtt tggtagagga tttataaaac aattaggaat
     1561 attaatagcc gttttttctg ggtatataat ctgtttactg ttaaggttag tagatataac
     1621 tactattaca gaagcaagtt tatttgcaat accgaacttt acagttccta agttcagttt
     1681 aggagcaatt gtgattatat ctccagttgt attagctgta tttatggaac atataggaga
     1741 tatgactact aatggtgctg tagtaggtaa aaactttata gaaaatccag gattaaatag
     1801 aacattactt ggggatggat ttgcgactat agttgcaggg tgtttaggag gaccagccaa
     1861 tacaacttat ggagaaaata cagcagtact tgctataact aaaaactatg acccttcaat
     1921 acttagacgt acagcaatat ttgctatatt gcttgcatgt gtaggaaagt ttggtggatt
     1981 tcttcaaagt ataccaggtt cagttatggg tggaattagt ataatgctat tttcaatgat
     2041 aacttatgta ggattaaaaa ctataaggga cagttcttgt gtagaaagta agataaatat
     2101 attaataata gcagttatat tattaatagg tcttggaaca acatatttat ctaataaagg
     2161 catttctatt ggaatcccta taacaagtac agtaaagata actggtctta gtttagctgc
     2221 aattgttgga attgtattaa acagaatttt aaataatcag gattttaaag ttgaagaaga
     2281 ataaatttaa agaataaatt gtatattgaa taactaaaat tagttaattt tctatagaca
     2341 tttgtaatta atgttgatat aatgattaaa aataaatgaa agaaagggca aggtaactag
     2401 gacaatgaga atataatcta ttttttataa tttaaagtat tgtaattttt gaaaagccta
     2461 ttttgatggc tttatttgca atgcttaaat taaggagagt agattatagt ctatacgttt
     2521 atttgttatg cctatttata gaatctctcc ttatatttaa gtgtgttaag gagggatttt
     2581 ttatgttgtt agtaaaagta gagaatttaa agaaatatta tgcagataaa ttaattttgg
     2641 atatagataa acttgaaata ttagaaaatg acaaaatagg tctagttggt tcaaatggtc
     2701 aaggaaaaac aacattatta aaagcaatat tgggtgaaat agaaattgat gaaggatata
     2761 cttaccttac agaaagctat tcttatataa gtcagagtga aaataatatt gaaacatgta
     2821 gtcatagtaa ggagaagagt cttttaaatg ctccagataa atttgaagaa tacttatcag
     2881 gaggggaaaa ggttaagtta aaaatagcag atgcgctgag taataagaaa aatatcataa
     2941 tagctgatga gccaacttca aacttagata aaaaaagcat tgggcttttg gaagatatgt
     3001 ttaaaaggca tgagggagca ttattattaa tatctcatga tagacgtttt ctggatgagt
     3061 tatgtacaac tatattagaa ctagaagatg gaaaactaaa agcttacaaa ggcaattaca
     3121 ctgattattt aatgcaaaaa gatgaagaag taaaaagagc tgattttgag tatcaagaat
     3181 atgttaaaga gaaaaaaagg cttgaaaaag ctcttttata taaaaaagct ttaagtgatg
     3241 gcataagaaa gacaccaaaa agaatgggta attcagaagc taggttgcac aaaatgggtg
     3301 gtcagactaa taagaaaaag ttggactcaa atgtgaaggc tataaaaagc agaattgata
     3361 aacttgaggt aaagaataaa cctaaagttt ctaaagaaat gaacattaag attcaagatg
     3421 gtatggagat aattagcaaa aatctattag aggtaaaaga tatgactcta aagttagaaa
     3481 ataagctctt gttagataat gtttccttta agataaaaag aggtaaaaag atagcattat
     3541 taggtgataa tgggtgtgga aaaagtactc tgataaaaga aatattggct gataaaaatg
     3601 acaatataaa aataaataac aaggtaaaag taggttgttt tgaccaaaat caaagtttat
     3661 tagatgagga aaagagtgtt ctatacaata ctaaagttaa tagttcattt gatgaatctt
     3721 ttataaggat aaacttaagc ctatttgggt ttaaaggtga tgatgtctac aaaaaagtaa
     3781 aagtacttag tggtggagaa aaggtaaaaa tagcactatg taaaataata ttggaagata
     3841 ataatttttt agtatttgat gaaccaacaa attacttaga tataaaatct atggaagcac
     3901 tggaaaaggc actgataaat actgataaaa ctatgcttat agtgtctcat gatagagtat
     3961 ttgtatcaca tatttgtaac tatattatag agataaaaga tgctaaaata agggagtttg
     4021 attgtaatta tgacgaatat ataattagca gaaataaaaa aactcctagt agagacaagc
     4081 aaatcaaaaa agagaatctt ctagtattag aaaatagact tacaactgtt atatctatgt
     4141 tatctataga aaaagataat ctaaaaaaag aattgtatga atcagaatat aatgaattac
     4201 taaaacaaat cactaaatta aaaaatagct tttagtgtta actttttaat tacatctata
     4261 taagaagact gaaaatattt gttgggaata taagtatagt tattaaaaca gaaaagtatt
     4321 ttgttagaaa attaaaagta agctaattag aaagtggcct tattattgta ctttataaga
     4381 aggccactat ttttatttag aatgataatg tgtgcttgtc taacatttat tttgtattta
     4441 gtgcagaatt tattcagatt taaccttaag cttggctact gtttcttctt caggagtaac
     4501 ataaatagta ctgttagttt cataaataac catacctggt ttagaaccac taggtttttt
     4561 cacatttttt ctcttagtat agtctacagg cacctgagaa gaaagttttg acttactaaa
     4621 gaatgctgct agcatagctc cttcaaatac agtattatca gggacttcct taccagcaca
     4681 ctttattatt acatgagaac caggaatatt ctttgtatgc atccataagt catcattatc
     4741 agcaagtctt agtgtaagat agtcattttg cttattgttt ttaccaacca atattttaaa
     4801 tccatcagaa gacacaaatt catgaggttt tgtagatggg atagtatcct ttttactgtt
     4861 tttcttttga gtcttgatat atccaacctt agcaagttct tcttttatat cttgtaattc
     4921 agctaaattt tcgcagtttt caatacttaa aactatgttt tctaagtaat ctatttcttc
     4981 cttatttaag gaaatttgat gagatatctc gactttagca tgtttcattt tgttatactt
     5041 tttaaagtat ttttgagcat tttcagatgg tgtcaagttt ttatttaatt ctattgttac
     5101 atcattacaa ttttcatcat agaagttagc aatttcaata ctttccatac ctttttctac
     5161 catataaata tatgaagtaa tcaattctcc ttttatctta taaatgtctg cattttcgga
     5221 ttctgataat tcttcttctt gttttttaag tttattataa agtctatcaa gcttaacaga
     5281 tatactcttc tttaaatcag atgaacgttg attaattctg tctttaatat ctttagttct
     5341 atagaaatct tctagaatac gagacatact atccttattt atatatgata aatcgctaaa
     5401 taatgtaagg tttatacaac tgaaatcaac taccttatcc acctttttat ctataataat
     5461 gcaaggggag tatttgttag aatttatatc attgaataaa ttacaaaata ctttgtgtaa
     5521 agaatcaaat tgttcatcag atatatcctt tatgatagca ttttgattta cacctgctct
     5581 gtagcaaatt tctttagcta taatagggct tatacctaga aatttagaat atatagactt
     5641 aaagataggt ccttccaatt cttctaggtt tttaataaac aaatccttag atatatcatc
     5701 aagagggttg agtttgtttt gttctggtgg taatgaataa tcatgaccag gtaaaacttg
     5761 acgaactcta cttatgctaa aagggactct ttttatagaa tctataattt tattttcttc
     5821 accatgagtt attattatgt tgctatgtct tcccataatt tcaatcataa tatttttaac
     5881 tgtcttttct tttaattcat ctaaagattc aacacttatt tttattattc tttcaaaacc
     5941 tatttgagaa atttctacta tatttccacc ttgtatgtat tttctaagta acatacaaaa
     6001 cataggcgct tttagagggt tttctttttg ataattactt gtaagataaa ctctaggatt
     6061 tgaggcactt gcacttaaaa ccaatctaaa attttcttta ttatttctta tattaaaaat
     6121 tacctcatca tcttcaggtt gatgtatctt atctattttt ccacctgtaa gttttgatga
     6181 taattcatcg actattgaat gtataactaa tccatctaat gccatttata gcaccccttt
     6241 ctttgaccaa aatgagtcag taaattaatt atacacataa tatacgcttt gtgactaggg
     6301 ggaatttaaa cgatagagat taatatttaa attgtataat tatttatact tattagagag
     6361 gaaactttat ttttaaaatt aataatttaa agtagattaa atataaagta aatatagtat
     6421 aattattgtg tagagtataa gctatgaaag tatatgaact ttacagaaaa aatcaatttt
     6481 aaggttctat agtatataaa tatgaaagaa ggttgaaaaa atgagattta tcgatttaag
     6541 aagtgatact gttactatgc ctactgacga aatgagagaa gctatggcaa aggctcctgt
     6601 tggagattca atctttagag atgaccctac tgtaaataaa cttgaagaat tagcagcagc
     6661 taaagttgga aaagaagatg ctattttcct tccaagtgga acttttggga atcaattagc
     6721 tctatttact cattgcctaa gagggcaaga aataattatt ggtaaaggtt atcacattgt
     6781 aactcatgag gttggggcac ctgctgtaat agctggagtt cagcttagaa ctatagatga
     6841 agacgaaagt ggagctctaa atcctgaact tgttgaaaag gcaattagaa gagatgacat
     6901 acatgaacca cagacagggc ttatatgtgt agaaaatgca tattcaggtg gaactgttgt
     6961 gagtcttgat aatttaagag aaattaaaaa aatagctgag aaacacaact taccagtaca
     7021 tttagatgga gcaagattat ttaacgcagc attaactttg ggtgtagaag ctaaagaaat
     7081 agcaaagtgt tgtgatagtg ttatgttctg tgtgtcaaaa ggtttagctg caccaatggg
     7141 ttctatactt gctggaagta aagaatttat agcaaaagct agaagaaagc aaaaagttat
     7201 gggtggggga atgagacaag taggtatagt tgcagcagct ggtataattg ccattgaaaa
     7261 aatgacttta agattgaatg aagaccatga aaatgcaaaa tatttagctt gtgaattaaa
     7321 taaaattgat ggcattgaag taaacaatgt taatccagat ataagtatgg tattttttaa
     7381 gatgagtgaa gatattataa aagaggatgt tttaataaaa gaattttttg aaagaaatat
     7441 aaaaattaat aaaatggaaa atggtgaata tagatttgta actcatgtag atattactaa
     7501 ggaagattta gactatgttt taaaaacact taaagaatta ataggtgtat gttaatagat
     7561 atctactaaa aggattatag ctggttacca gatgtttaat taaaaaattc atagacagtt
     7621 taaataaaac atacataaaa ttcatataga aatattaata tataaacata gaaagaacta
     7681 gcttaaaaac ataatataat taccctaatt gtaatgacaa ctctccctca catcctcaaa
     7741 ttatagttgt cattacaaaa cttttcccat aaattataag tttatgggaa tttttttatg
     7801 ttataatagt ttaagcgtta atttacgaag gagcgatact tatgaaattt tggaaactac
     7861 atggaatagg aaatgatttt atagcaatag atggaagatt cgaccagata aatccatctg
     7921 attatactgc tttagcaaaa aaagtatgcc atagaagatt ttctgtagga gcggatggat
     7981 tgttagttgt gaaagattca aaagttgcag atgtagaaat ggtatattat aattctgatg
     8041 gttcaagagc agctatgtgt ggaaatggcc ttagatgttt ttgtaaattt gtttatgata
     8101 attgtatagt taaaaaagaa agttttacag tagatacatt agatggtata aaagatataa
     8161 aaataaatct taccaataga gaaataaact ctataagagt caatatggga aaaggaagtt
     8221 ttatagctaa ggatgttcct gtacttacaa ataaagaacg ttttgtgcaa gaaagtataa
     8281 aggttttaga tagggagctt aaagttactt ctatgttgat gggggttcct catacagtag
     8341 ttatagtaga tgaattagat gtagatgatg tttgtaaata tggaaaagag attgaaaata
     8401 ataaaatatt tcctgaaaaa acgaatgtaa attttgtaaa ggtagaagat aagtataata
     8461 tacatgttta tacttgggaa agaggttgtg gatatacttt aggttgtggt actggtatga
     8521 ctgcatcagc aatagtgtgc aatttactta atatggtaga aagtagtgta aatgtaacgt
     8581 ctcaaggtgg aacagttaaa atagatgtag gagatttctc ttatatgaca ggtccagcag
     8641 taaaaatatg tgaaggtgtt ttggaggttt aattaatggc tataagtatg actggttttg
     8701 gaagaggaga atacaaggat gataattatt actttttagt agagtgtaaa acgataaatc
     8761 ataagtattc agatataaac atacgtcttc caagaaaaat ttcattttta gaggataaag
     8821 ttagaaattt agtaaaaaat tatgtaaaaa gaggaagagt agatttatac ataaagtttg
     8881 acttattagg caaagaagat gtaaacttaa attttgatga gggattagca tctcagtata
     8941 tagacatact aaaagaaata aaaaataaat ttgatattat agatgatatt agcgttatga
     9001 atgttgcaaa gtttcctgat attgtgaaaa tagaagaaaa ggaagaagat gaagatttat
     9061 tgtggtcaat gttgaatcaa gcagttgaag atgcattaat caaattaaga gaaatgagaa
     9121 gtgaagaagg taaaaaatta gctgaagata ttgccatgag atgtgattta ttaaaaaatc
     9181 atatagaaga gattgaaaaa tattcaagta gtgttgtaga agattataga gaaaaattga
     9241 atttgagaat aagtgaatta ctggatgacc caagtataat agatgagaat agattagcac
     9301 aagaagttgc aatatatgca gataaaagca gtataacaga agaaatagtt agatttaaaa
     9361 gtcatatagg gcaacttaaa aatactatat ttaaagacga ctctataggt agaaaaatag
     9421 atttcttaat acaagagatg aatagagaaa ctaacactat aggttcgaag tcatctgata
     9481 taaatataac taatttagta gtagaagtaa aaagcgaatt ggaaaaaata agagaacaaa
     9541 tacaaaatat agagtaagaa tacaatatta gaaggggaat gcttaaatgt ttgtgaaaaa
     9601 aggattattg ctagttgtat caggaccatc aggtgcagga aaaggtacta tttgcaaaga
     9661 actacttaaa gaaaatgata caataaaact ttctgtatca gctactacaa gaaaacccag
     9721 aactggagaa gttgatggag taaattattt cttcataagt aaggaaaagt ttgaagagat
     9781 gatagaaaaa ggtgagtttt tggagtatgc tcaaatttat gataattttt atggaactcc
     9841 aaaagcggca ataatggaat gtttagaaaa aggacaagat gtattactgg agattgaaat
     9901 gcaaggagca aaacaaataa aagaagtttg cccagaggga gtatttatat ttgtactacc
     9961 accatcactt gaagaattaa aaaatagaat tgttggaaga ggtacagaaa cagaagctga
    10021 aatagagaag agatttagct gtgcatatga agaaattaaa atgattaagg attatgatta
    10081 ttttatattt aatgaagatg taaaaacatc tgctaaagaa atagaaggta taatatcatc
    10141 tgaaaagaat aaagttagca gatataaaaa tattataata gagaaattta aggaggaatt
    10201 ataattatgt taaaaccatc tatcaatgag gtactagaaa aaatagacaa cagatactat
    10261 ttagtaggga ctgtttcaaa gagagcaaga aaattaattg atggagaaga accatatgtt
    10321 tcaaataaaa ctaaggaaaa gcctgtatgt gtagcaacta aagaagttgc aagtggaaaa
    10381 ataacatata gattattgac agaagaggaa atagagatag aagaagctag acatcatgct
    10441 gagcaacacc aacaaatttc agaagaggag taaggtcatg ctaaaagata aaactgttgt
    10501 aattggcata agtggcggta tagctgcata taaggcttgt gatgttgtaa gtaagttaaa
    10561 aaaactaaat gcaaatatcc atgttataat gacaaaatca gcaacagaat ttgttaaacc
    10621 acttgcattt caatcgctga gtcaaaatta tgtagtagaa gatatgtttg aagaaccaaa
    10681 aacttgggaa gttgaacata tatcattagc aaaaaaagca gatttattct taatagttcc
    10741 agcaagtgca aatgttatag gcaagattgc aaatggtata gctgacgata tgttgacaac
    10801 tactgttatg gcaaccaaag cacaggttct tatagcacca gctatgaata ctaatatgta
    10861 tgaaaatcca attgttcaaa aaaatataca aactttaaag agctttggat ataaatttat
    10921 tgaaccagaa agtgggaggc ttgcatgtgg agatattgga acaggcaaat tagctaatcc
    10981 agacataata gtagaggaag ttttgactaa tcttactaaa gagcaagatt taaaaggtca
    11041 aagtatgata gttacagctg gtccaactgt agagagtata gacccagtaa gatatttaac
    11101 taataggtct tctggtaaaa tgggatattc tatagcaaga gaagctatag ctagaggtgc
    11161 tgatgtaaca ttaatatcag gtcctacaaa tctttctatt ccacaaaatc ttaaaaaatt
    11221 tgttcaaata gagtcagcac aagatttgta tgaagcagta gtatcaaatt taagtgaaaa
    11281 taaaatagta attcaaagtg cagcagttgc agattataaa ccaaaaactt attctgacaa
    11341 aaaaattaaa aagaagaatg atgatttatc aatagaacta agtagaaatt atgatattgc
    11401 ttatgaaata ggaaaaataa aagaagataa gatactaatt ggatttgcag ctgaaactaa
    11461 tgaccttata gaacatgcca aaggaaaaat aaaaaagaaa aatttagatt ttattgtggc
    11521 aaatgattta actaaagaag gtgctggttt tagagctgat actaatattg taaaaataat
    11581 agatgtagag ggaaatatta ctgagtatcc taagatgaaa aaagaagaag tagccaatgt
    11641 tattttagat aaagttaaag cgttgcttga gaaataagac cttttttaaa tatgatattt
    11701 gtgtatttta tcacagctaa agtggctgaa agctgcaatt taattttagc gagtaaaaac
    11761 tcgcaaatgt cagttaaatt gtagttttca gcatttattt tgtcataaag tataaaatat
    11821 aattaatagt taattattaa aataaagtta atattaataa cttgaaattg cttataaatt
    11881 ctgagatttt aatatcttgt aaagtatatt aaaatatgaa caatatatta aatttaagta
    11941 gaggttttat aatgaaaaaa tatgcaaagg taatagttag aagtaatact atatatacag
    12001 ataatttatt tacatatcaa attcctgtat ttttatctga tgtaataaaa atcgggcaca
    12061 gaatattagt accatttgga aaaggtaata aaccaacaga agcctttgta tttcaattta
    12121 cagatagctt agatgaaaaa ataaaaataa aagaaattat tgatatatta gatgaaaatc
    12181 caatatttag aaaagaagat ttagagttgg tttattggat gaaaaataga tatttgtgta
    12241 cttatataga atgtataaat ttgatatatc caaagggata caaattaaat aattataaag
    12301 tagtcctatt aggtgaaagt ttaagtggat taaatgagat tgaattgaag gaaaaaatat
    12361 caactttaag tgataaaaat agagaaatag tagagaaagt gattgatagt aaaggtaaaa
    12421 ttaaggttga taagttaaaa tatataccaa acttaaatag ttctttgtac acaatgaata
    12481 aaaatggaat tataaaatta tgttgggaat ataaaaatca caaaaatgaa aagaaagtat
    12541 gttatatatc tttgagttta gaaagtgata agattgacga ttatatagag caaaataaaa
    12601 taaatgtggg aagtaaacaa aaagagatat taagttttct taaaaataat gaaaatgtag
    12661 agataaatga tttattggat ttattaaatg cttcaaaaca aagtataaat tctctaagta
    12721 agaaaaagct aataacccta gaatttaagg attattatag agagccaaaa agtatttaca
    12781 aatccgtact aaaaagtata aaattaaaca atgaacaaca agaagcagtt gatgagataa
    12841 agtcgaatat gtttgtagat gataaaaaac catacttaat tcatggagta actggaagtg
    12901 gaaaaactga agtttacatg gaaataatag aatttgctct aaatcaaggc ttagacagta
    12961 tattcttagt acctgagata gcattaactc ctcaaacaat agataggcta aagagtaggt
    13021 ttggagattt agttggagtg ttccacagta aactatctga aggagaaaaa catgatgttt
    13081 acaaagctgt caaagcaggt aaagtaagag tgttaattgg agctaggtca gctttatttg
    13141 caccttttaa ttccttaggc ttaataataa ttgatgaatg tcatgagtca tcatataagt
    13201 ctgagaagaa tccaaaattt aatgctatag aagttgctag atttatggca ttaaaaaata
    13261 atataactct tattttaggt tcagcaacgc cctcaattga agagtattat agggcaaaat
    13321 ctggagaata caaacttata aatataaaaa gtagagcaaa tgataagcct cttccaaaca
    13381 ttgaagtggt agacatgaaa gatgaattag ataaaggaaa tagaagtata tttagtttga
    13441 aattacaaaa agaaatacgt tatgcgattg aagaaaataa tcaagtgata ctatttttaa
    13501 acagaagagg ttatgcaaat tttgtatctt gtagaaagtg tggatatgtg tttcaatgtg
    13561 aaaattgtga tatatcttta acttatcata agaaaagcaa tactggaaga tgccactatt
    13621 gtggatatga gaaagaaatt ccaaaagagt gtcctgaatg taaaagtact tatgttaagc
    13681 catttggagt tggaactcaa aaaattgaag aggaattaaa atatattttt ccagatataa
    13741 aaactttgag aatggataaa gatacaactt caaaaaaagg tgctttagat gagatactaa
    13801 ataaatttaa agataaagaa gcagatgtac ttattggaac acagatgtta agtaaagggc
    13861 tggattttga gaatgtaact ttagtaggta ttttgtctgc agacatgata ttaaattttc
    13921 cagactttaa aagttttgaa actacatttc aattgataac tcaagtctct ggaagagcag
    13981 ggagagctga taaagaggga aaagtagtac ttcagactta tgacactgaa cactatgcta
    14041 ttaagcatgc tattgaatat gattatgagg gtttttatga agatgagata aaaataagaa
    14101 aagcatttgg atattctcct tttaataata tgttaagtgt agttgttagt ggtgaagatg
    14161 aaagattagt gataaaaaat ataaaaaata tgcatgcatc attgatttat ttattagaaa
    14221 aaaggggtat aaatgattta ggatttatat taggtccaaa tccatgttca atatcaaaaa
    14281 taaatcaaaa ttatagatgg caaatactct ttaaagatga aaatattgaa attaatctct
    14341 taaagggtat aataaaatat atatgtataa caaaacgaga tttgatattt gataagaata
    14401 tcaatgtatc tatagatatt aatccaaaca gtgtattata aatttaggag gaattataat
    14461 ggctttaaga caaatagttc aaataggaga accagtatta agaaaaaaat caaaaaaggt
    14521 tgaaaaaatt gatgaaaaga taatacaatt attagatgat atggcagaaa ctatgtatga
    14581 tgcagatggt gttggactgg ctgcacctca agttggaata ttaaaaagag tagtagtaat
    14641 agatattgga gaagagctta tagaacttat aaatcctgaa ataatagaaa catctgggga
    14701 gcaaatagat gaggaaggat gtttaagtgt tgttggagaa gctggaaatg ttagaagacc
    14761 aaattatgta aaggtcagag ctttaaatag aaatggcgaa acaatagagt tagagggaga
    14821 agaactttta gctagagcat tttgtcatga gatagaccac ttagatggaa ttttatttgt
    14881 tgacaaaata gaaaagtagt aggagttgat ttgatgaaaa tagtatttat gggaactcct
    14941 gatatagcag ttccatgttt acaaaaaata atagatgaga agtatgaaat attgggtgtt
    15001 gtaactcaac cagataaacc aaaaggaaga ggtaaaaaac taggtatgag tccagtaaaa
    15061 gaactggcaa ttgaaaataa tatacctgta tatcaaccag taaaggctag agataaagaa
    15121 tttatagata aaattaaatc tttaaatcca gatgtaatag tagttgtagc ttttggacag
    15181 atacttccaa aagaaatatt agagattcct aaacttggat gtataaatgt tcatgtttct
    15241 ttacttccaa aatatagagg tgcggcacct ataaattggg taataataaa tggtgaagaa
    15301 aagactggtg ttacaactat gtatatggat gaagggttag atactggaga tatgatacta
    15361 aagacagaag taaatcttga tgaaaatata acagcaggag aacttcatga taaaatgatg
    15421 aatataggag cagaaacttt aaaagaaaca ttaagattaa ttgaagaagg aaatgctcca
    15481 agagaagtac aaaatcatga agaattttcc tatgcgccaa taatgaataa atcattaggt
    15541 aacatagatt tttcaaaaag tgccagagaa atacacaatt tagttagagg agtaaatcca
    15601 tggccaagtg cttatactat ctataatgat gtcataatga aagtttggaa aactaaagtg
    15661 ttagatgaaa aaagcacaaa agatgtaggt acaataattg atgtaagcaa agatgggata
    15721 aaagtaagta ctatagataa tgtactttta attgaagaaa ttcagatgcc gaataaaaag
    15781 agaatgttgg ttggtgaata tataaaaggg aatactatag aaactggttt agtattgggt
    15841 taggtgaaaa gtatgacaga tataaagaag tatttaacaa gtgttgggat tttgatttta
    15901 atattaggaa ttttgatagg agttgttaat ttttttataa taagtttaac tcaaatattc
    15961 tccttgatga taaatattat aataatatca ctaatactta taatactatg ttcaacagca
    16021 gttacttata gagtaattaa aggaaagtat gttaactcaa atataatgaa aataaatttt
    16081 aatattgtca gtggattatt tcctattata tctttcattg catcatcatt tggtatgtca
    16141 aaaagtgata ttagaagaat atatataaag ttaaataatg aatatatata cagtaataaa
    16201 tataatttta atccagagga tataataatt ttaatacctc attgtattca agagaatagt
    16261 tgtaaattaa aagttactaa tgatatagat aattgtaaag aatgtggaag atgcaacgta
    16321 ggagaattaa ttaagctaaa ggaaaaaact aatgtaaaaa tatttgtagc tacaggagga
    16381 accctagcta gaaaaattat aatggataat aaacctaaag ctgttgtagc tgttgcatgt
    16441 gaaagagatt tgacatctgg aatacaagat attaagaaaa ttcctgtttt aggtgtattt
    16501 aacaaaagac ctaatggacc ttgtgtaaat actaatgtag acatgatgga tattgaaaaa
    16561 gctatagact ttttaacagg aaaaaatata ttaatttgta attaaactgt ttttaagtta
    16621 agtgaatatt aagaggatgt ataataaaaa tatattttag gagagtgtta tttatgtacc
    16681 ctgtatatgg cggtttttgg ggatttgacc caacaatggt tgtattaatt ccagctatat
    16741 tacttactat atatgcacaa tttaaagtga gttcaacaac aaataagtat ttaagagtaa
    16801 atacacgtcg aggatatact ggagagcaaa cagcaagaag agtacttgat tcaaatggtt
    16861 tgtatgatgt aaagatagaa atggttagag ggcatctaag tgaccattat gacccaagaa
    16921 gaaaggctgt aaggttgtca gaagatgtat attatggaac atctataact tctgttgcag
    16981 tagctgcaca tgagtgtggt catgccatac aacatgctaa aggttatgcg cctcttcaaa
    17041 taagaagtag tttagtacca gtagtgaatt ttgcttcaag tatatcttgg tttttaatat
    17101 ttttaggatt tattatggct ggtccatttt taaaaattgg tatactattg ttttcggctt
    17161 cagtgttatt ccaaataata acattgcctg tagaatttaa tgcttcaagt agagcaatcg
    17221 ttcaacttgg gaatttaggt attatagatg aaagtgagtc aagacaaagt agaagagtac
    17281 tttcagcagc agctttaaca tatgttgcag cagcattggt ttcaatactt caattactta
    17341 gattgttact aatagctcaa agaagaaatg actaaaagta aagtgggttg tttagaaaca
    17401 accctttttt atgtttaata aaagggagaa atttatatgg atgcaagaga agttggattt
    17461 aaagtattat gtgatataga aaaaaatgac aattattcaa atattgcaat aaataagcat
    17521 tttaaaaatt tagaaataag tgatatggat agaggacttg caacagagtt aatttatgga
    17581 gtaatagaaa ataaatatta tttagattat ataatcaata aactttcaaa aataaaggta
    17641 aaaaaaatgt caacttatgt taaaatcttt ttaagaatgg gaacatatca gattttattt
    17701 ttagatagta tatcagatta tgcagcagtt aatgaaactg taaaattatc taagaaatat
    17761 gataaaaaat cttctggttt cataaatgct attcttagaa atgaaataag agctaaagag
    17821 actataatgg acataactga ggaagatagt gtaaaatact tatctataaa gtattcctat
    17881 aactcttgga ttataaaaaa ctggatagat aaatttggac aagaatttgc tgaagatttg
    17941 ttggaagcaa ataatgaaaa gcctagtatc tatataagaa cgaatacact taaaataagt
    18001 agagaagaac ttattgaaaa attaagtgaa atgggtattg tatgtttaaa agttcctatg
    18061 gtagaagaag ctattaaagt cgaaaaaatg aaaaatatag aaaaaaatga attatttaag
    18121 gctggtttat ttacaattca agatataagt tctatgatag tagggaaagt aattaatcca
    18181 aaagaaggtt catttgtttt ggatgtatgt agtgctcctg gagggaaatc tactcactta
    18241 gctactttaa tgaacaatac gggtcaagta gtagctagag atatttttga gcataaacta
    18301 aagcttataa actctactgt aaatagattg gggcttaaaa atgtgtttgt agaaagattt
    18361 gatgcttctg agattgatga aaatagtata agtaaatttg attatgtttt agcagatgtt
    18421 ccttgctctg gttttggtat aataagacgt aaaccagaaa ttaagtataa gaaagaagaa
    18481 gagttaaaag acatcacttc tatacaaaag aaaattttag aaaatgcatc taaatatgtc
    18541 aaagttggtg gaactttagt atatagtaca tgtacaattc aagatgtgga gaatataaat
    18601 atagttactt cttttgttga agaaaatcac aactttgaat ttgttccaat tgatacagta
    18661 aatgtagact tagacaatca agataaagga tatttaaaga tatatcctaa tattcatggt
    18721 atagatggtt ttttcatagc aaaattaaag agaataaggt agtgagagaa tggaaaagaa
    18781 aaagatagta ttaaaaaatt ttactgaaga tgaacttaaa gaatttatga aaactataga
    18841 tgaaaagccc tttagaggaa gtcaaatatt ctcttggata tataagggtg caaaaacttt
    18901 tgatgatatg aacaatatac caaaaagttt gagaaataaa ttagaagagg tttcatgtat
    18961 agggaatata gatatagaat taaagttaga atctaaggta gataatacaa aaaaatattt
    19021 gtttttatta gatgatggaa atataataga aactgtgatg atggattatg atagtagagt
    19081 tactgtctgt gtttctaacc aagtaggttg taggatggga tgtaattttt gtgcatctac
    19141 aatggatgga ttaattagaa acttagagcc atgggaaatt ttagaccaag ttattaaaat
    19201 tcaagaagat actggtaaga gggtatcaaa tcttgtattg atggggagtg gtgagccact
    19261 tgataacttt gaaaatacaa aacaattctt aaaaataatt aacgaaaaaa atggtcttaa
    19321 tatagggtat agacatataa ctctttcaac ttgtggaata gtacctaaaa tgtatgagtt
    19381 ggcagactta gaaatagcta taaatttagc tttatcactt cattcaccat atgatgaaga
    19441 acgaagaaaa attatgcctg tagcaaatgc ttattctatt gaagaaatat tgaatgcatg
    19501 tagatattat ataaaaaaga caaatagaag ggttacattt gaatattctc ttataaaagg
    19561 agtaaatgac tcagaaaaag aagcaaaggc attagctaag ctcctaaaag gtatgctatg
    19621 tcatgtaaat ttaattccaa taaataaagt tgaagaaaga gaatacgaaa agccagataa
    19681 agcatttatt tataaattta gggatagttt ggagaagaac aatatacctg ccacagttag
    19741 gatgtctatg ggttctgata ttagtggagc ttgtggtcaa ttgagaagaa aatacaagta
    19801 atttgtaagg aggaagtcgt atggtttata gttgtgcctc ccatatagga aaaataagaa
    19861 aaaacaatga agactattgt gagggagaag ttatagatac agaacatggt ccaataggaa
    19921 tatttgccat agctgatgga atgggtggac ataaaaaagg tgaagtcgcc agtaaattag
    19981 cagtagaaaa tataattgat tttctaaagg aaaacttatt acaacatgat aatgtaaaaa
    20041 tagactatat agatgatata ttaaaacagg cttataataa tgtaaactca attgtgcaca
    20101 aaaaatctat ggaagatata gaatttgaag gaatgggaac tacattagtt acggctatag
    20161 tgtataataa tgtcttatat gtggcaaatg ttggagatag cagatgttat ttgttaacag
    20221 atgaaaaatt tgataaaata actatagacc attccgtggt tgaagaactt atgatggccc
    20281 atgtcattac agaagaagag gcaagaagac atccacagag aaatagaata actagagcaa
    20341 ttggaacaga tgatatggtt gttgtagata tttttaagaa agaaatcaaa aaaagtgata
    20401 taatacttct tgcgacagat ggattaactg ggtttattga tgatgaagat ataagagatt
    20461 taatattaga ttatgaatat gagagaacta gtaatataag tgaagaattg ataagtatgg
    20521 ccaatgatgt ttctggtaaa gataatgttt cggttatagt gataaaagta taattaggac
    20581 ggtgatattg tgggagatac aattttagga aatcgatatg aaatcatcag gaagattggt
    20641 gatggaggaa tggcctttgt atatgaggct aaagacagat tattaaacag gactgttgca
    20701 cttaaagtac ttagacctga atttgtagat gatgatgaat ttctaacaaa gtttaaaaga
    20761 gaggcagaag cagtagcaag tctttcacat ccaaatatag taaatgttta tgatgttggt
    20821 gaagatggaa aagttcacta cattgtaatg gagtttgtag atgggaagaa tctaaaagag
    20881 attatacaag atgaaggtat attagatgaa tatactgcat tagatataac aaaacaaata
    20941 gctatggcac ttagtgcagc gcacaaaaag gggattatac atagagatat aaaacctcat
    21001 aatattctca tatcaaatga aggaagagta gtaaaagtgg ctgattttgg tatagccaaa
    21061 gctgtttcaa attcaacaat gactaatatt ggtagtataa taggttcagt gcattatttt
    21121 tcaccagagc aagctaaagg taaatttgta actaataatg ctgacctgta ttctctaggt
    21181 atagttttat atgaaatgtt gataggaaaa gttccattta ggggagatag tccaatttct
    21241 atagcacttc aacacattaa tgatgatata gactttacat cagaagaaaa agttaggatt
    21301 cctcaaagtg taagaactac aataaaaaag cttacagaaa aatctagcgc agatagatat
    21361 caaactgctg aagaattgat agaagatata gagtatatag aaaaaaatat tgatttagat
    21421 tttataaaag aatatgatga ttttgcaact aagaaaattg atgaaaaaga aattaataaa
    21481 gttgtaaatc caacacttgc aaaaccagct cctgaaaaag tggttaagcc tgttgaagta
    21541 gctgatttgg atgatgatga agattactat gatgattttt atgaagaaga tgaagaagaa
    21601 gaagaagaag aggaagagat aatgagagct aaaaagaatc agagacctaa gagtactcca
    21661 agtaagagaa ctaagaaaaa gaagaaaaaa caggaaagcc caaaatctag aagaagatta
    21721 aaagtaattg cggctgtttt gatactgata ttgtgtgctc aagtattctt agcatataag
    21781 tttttatttg ctggagggtt tggcaataaa tctttgactg ttcctaatct tgtaaatatg
    21841 acattagaag aagctcaaag tgctgttgaa aaagaaggat tatacctgag tgtaaaaagt
    21901 gaagagtata atagtgaagt tgatgaaaat tgtataatat cacagacacc tgaaggtgga
    21961 agtacaaatg tcaaaaaggg tgatactata aatgttgtcg taagtaaagg ttcaagtcag
    22021 gcatctgttc ctaatgtggt gggacttact ttgagtaatg caaaacaact tattgaagaa
    22081 aataacttaa aagtgggaac tgttaagtat gaatatagct caatatataa agaaggtaca
    22141 gttttaagtc agagtccagg tgctggctca tcaagagctc aggaaggtga tgaggtaaac
    22201 ctatatgtaa gcaaaggttc agaaaaatca aatacacaga ctccaacagt accagataaa
    22261 aaaccaacaa caccagagaa taatacacca acagaacctg gttcaaactc agggaacagt
    22321 ggtggaaatt caggaagttc aaactcagga aatagtggag gaaactcagg caataatgga
    22381 ggtaactcag gaggttcaaa ctcaggaaat agtggaggaa gctcaggagg ctcaaactca
    22441 ggagataatg gaggaagttc aggaggttca aactcaggag ataatggagg aagttcagga
    22501 ggttcaaact caggagacaa tggagatagt tcaggaggct caaactcagg agataatgga
    22561 ggaacttcat caggaactgg cgcaaacatt ggtaaaacag aataaatatt aataaaataa
    22621 gagctgtcct tttatagaaa atattttctt taaagggcag tttgttttgt ataaaatact
    22681 gaattggtta taatataagg agaaaagtat ttatgaagaa gaaaaccgtt ttaattacag
    22741 gaggagctag agggattggt aaagctatgt ctaaagcttt tgcaaaagaa ggttacaatg
    22801 tattagtaaa ttttaataag tcagaaaatg aagctaaaga gttgtatact atattaaatg
    22861 aaaaaaattt ttctgtaaaa ctttttaagg ctgatatttc aaatagagaa gatgtagaga
    22921 atatggtaga ttattgtata aaagaatttg gaggattgga tgtacttgta aacaatgctg
    22981 gtgtaagtca agataagttg tttacagata taaccgatga agattgggat aatatgatga
    23041 atattaattt aaaaggaagc ttttattgtt cgcaagttgc tttaaaatac atgatatcag
    23101 aaaaaaaagg aaatataatt aatatatctt caatctgggg tatatcaggt gcgtcttgtg
    23161 aagtgcatta ttctataaca aaagcaggta taataggtat gacaaaggca ttagctaaag
    23221 aagtaggacc atcaaatatt agagtaaata gcatagctcc aggtgtcatt aatactgaca
    23281 tgttatctgg atataatgag gaagatatag acgctcttgt ggaagaaacc cctctaatga
    23341 ggctaggaac tccagaagat atagcaaatt gtgctatttt cttggcatca gacaaatcaa
    23401 attttatcac tggtcaggta attagtccaa acggaggatt tgttatttaa aaaaaatgtg
    23461 gtattatata aaaataggaa agaataacac gatatactat tcataaacag tatgtgttta
    23521 tataaatttt ataaagggag gcatttgatg ctagaaggaa aaattataaa ggggattagt
    23581 ggattttatt atgtagatac ttataatggt atttatgaat gtaaggctag agggatattt
    23641 agaaagcaga aaataacacc tttggttggt gatagagtca aaataagcat tgttgatgaa
    23701 gatgaaaaaa agggtatctt agaagaaatc gacagtagag atacagaact tataagacct
    23761 cctattgcaa atgtagataa agcattaata gtattcgcaa taaaaaatcc taaaccaaac
    23821 ttatctcttt tagatagatt tatagttttg gcagaaaaag aaaatttaga aactgtaata
    23881 atccttacaa aagcagattt agatgataat gatacattag aaactgttaa aaatatatat
    23941 gaacttagtg gctataaagt cattcctgta agcaatatta ctaagttaaa tatagataaa
    24001 gtaaaagaag aattaaaaga aaatgtagta gtgtttgcag gaccatctgg agttggaaaa
    24061 tcaagcttat taaatgaaat agatgaaaat tttaaacttc aaacaggagt ggtaagtgat
    24121 aaaataaaaa ggggaaaaca tactactcgt cacgctgaac ttttaaaatt ggaatttggt
    24181 ggaatggttg cagatacacc aggttttagt tcccttgcac ttgaagatat agaggaagtt
    24241 gaacttaaag attattttat agaatttgat aaatttaatg attgtaagtt tggttcaaag
    24301 tgtattcatg aaaatgaacc taattgtgct ataaaagaag ctgttacaaa tggagaaata
    24361 tctaaagaaa gatatgatag ttatatacag ctgctacatg aaataagaca aaataatagt
    24421 aggaggtatt agaaaaatga ttaaattagc accatcaata ttatcagcag attttgcaaa
    24481 attattagaa gatgtaagaa aagtagaaag tgcaggatgt gaatatcttc atatcgatgt
    24541 aatggatgga cactttgtac caaacattac attaggacca cttgtagtca aaagtttaaa
    24601 gaaagaaaat ataaatatgg tatttgatgc tcatcttatg atagaaaatc cagaccagta
    24661 tattgaagaa tttgtaaaag caggatgtga tataataact gttcaccagg aggcttgtgt
    24721 tcatctacat agaactatac aaaatataaa atcacatgga ataaaagctg gtgtagtact
    24781 taatccagct acaccagtag atacgattaa acatgtacta cctgatttag atatggtatt
    24841 attaatgtct gttaaccctg gatttggagg acaatcattt attccttgtg ttttagataa
    24901 gattaaagag ttaaaagcta ttatagatag tcaaggatta aacattgata tagaagttga
    24961 tggtggtata agccctaaaa acgtagccga agttgtacaa gcaggagcta atgtaatagt
    25021 agcaggttct gcaatatttg gaagtgatga cattcaagaa acagttaatt tatttagaaa
    25081 gaatgcttct cttgaagaat tagtttaaat ttaataatga aaatctgtat tgttttaaat
    25141 ggcgaaattg aagattacga agttacaaga gatattataa ttaaggaatg ttatgactgc
    25201 ataatatgtg cagatggagg ggcaaatcac acttataaaa tggaaataat gccagattat
    25261 atattaggag atttagattc agtagaagaa gaaaagataa acttctataa aaataaaggt
    25321 gttaaatttg aaaaatttcc atctaaaaaa gatgagactg atacagagct ttgtcttttt
    25381 ttagctaaga cacttaaagc aaatcatatt gatttttttg gtgctttagg gggaaggatt
    25441 gaccacactc ttgcaaatat aaagctatta tattatttaa aagaagatgg tatatattct
    25501 aggatacttt ctgataaaga agagatgtat atagttgaaa atgaggaaat ttcattgtat
    25561 ggaaacccag gggacactgt ttctgtaaca gctataaaag gtgatgctaa gggtgtaaca
    25621 cttactggct tagaataccc tctcgataat tattatatga aatactctgt acctattgga
    25681 atatcaaatg ttatgttgag taattcttgt aagattaaag tagagcaagg ctgtgtactt
    25741 gtagttagaa atttataaaa tttgattttg acaattggtt ttatattgta gagcagatac
    25801 aatcaagtaa aatatattta tgattgtatc tgctttttag tatataatta taaatatata
    25861 ataaactttg acataagggg gattttttgt ggtagatagt attatttttg atttagatgg
    25921 aacattgtgg gattcaactg aagaagtatg caaagtctgg caggatgttt taagtaaaca
    25981 taaggaaata gaattaagtg tcacaaaaga tttatttaga agtcttatgg ggctttcttt
    26041 tgaggaaata gcaaatcgtt tatttccaaa tttaagtgaa gaagaaagaa tgaatttatt
    26101 gaatgaatgt tctttgaaag aatgtgacta tttatctgaa aatggagcta aattatttga
    26161 tgatatagaa gatactctta aattactatc taaaaaatat aaattattta tagtaagtaa
    26221 ttgtcaagct ggatatatag aatctttttt aaaagcacat aaactagaac agtattttat
    26281 agattttgaa tgtcctggta atactggttt gcataaagga gaaaataata aacttataat
    26341 tgaaagaaat aaattaataa atccaatcta tgttggtgat acgcaaggtg atgcaaatag
    26401 tgctaagttt gtaggtatac cttttatata tgccaaatat gggtttggga atgtagatgt
    26461 atatgattat tttatagatt catttaaaga tttgttagaa catgatatat taaaataaga
    26521 tgagtttaaa aattatatta aaataataaa aaagttatgg ggggatttaa aaatggaatt
    26581 acaagatact atttttaaaa gacaaagtgt aagaaagttt aaaaatcaag atgtatcaga
    26641 tgaagatatt ttaaaaatga taaaagcagc aggtgcagca ccatcaggca agaatattca
    26701 aaactggcat tttgttgtta taaaacgccg tgatttaatg gaaaagatag cagatgtgat
    26761 aactaaaaag caacaagaaa tacttgtgga aatggataaa gtatcagtgg ataaggctaa
    26821 tagatttaga aaatttgtta aaaactttac actattttac ttaaaagctc cagttttagt
    26881 attagttttt acaaaggtat ataatccatc aggatattat gaattagagc ttatagatgc
    26941 acctaaggag actatagata aattatttat aagaaatcca ggtatgcaga gtctaggtgc
    27001 ttcaattgaa aactttacac tatcagcaat tgaacttgga tatggttctt gttggttgac
    27061 aagtcaaaac tatgcagcag atgagataga agctgtttta gaagcagaaa ctggttttga
    27121 aaagggagag tattttttag gtgcaatgtt agctcttgga gtgccagagg acaatttgaa
    27181 gagtccatct aaaaaaccag tagaagaaat atgtacattt ataaaataaa agtcaaggac
    27241 attttaaaat caaagtaaat taaaaacaag ataaattaaa aataaatatt taaaaaatta
    27301 aaaagtaggt tttattgata ttaattttat atttcaaatc attgatttaa ttaatacaat
    27361 aagaacta
//
LOCUS       DAMJWP010000027        26984 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.028, whole genome shotgun sequence.
ACCESSION   DAMJWP010000027 DAMJWP010000000
VERSION     DAMJWP010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26984
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.028"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..1322
                     /locus_tag="PC700_001034"
     CDS             <1..1322
                     /locus_tag="PC700_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2580354.1"
                     /translation="KIYFFIIGKIYFLFLDDALIAKVNFLKARKYADLLKDMQVSSLC
                     NIKIGECECSFENYECSIEYLNEVISNKKYRNVNIIQKYRASNNITKILIKTRNYPVA
                     INNLAKSEVYLEKIKNQSLKEVEKFDLFISLAMYYAKSNEKSFEQSICYLDRAKNILN
                     MIKNIEEYTIYDLEIIYHQAKIYYIFGYYDNALNTNKKLLEKSKNAKNYNYVKLAYKN
                     IYLCFEKIQNYEMSMKYFKMYYELKMMYIKARNRNYIDSLNYRHEYIETEINNMRKVK
                     LDLDKKKYVDHLTSAYNRTFLNDFLEKQEVSEYCTAFMIDVDYFKEYNDTYGHYNGDI
                     ALKNITIIIKKYLKKDMFMIRYGGEEFLILSVCKDYKKSKIFGRKLCRIVKKFLNNNL
                     TVSIGIDTCKNSSIDIREIIENADKALYKAKESGRNRCLHYHDFKNF"
     gene            1349..3235
                     /locus_tag="PC700_001035"
     CDS             1349..3235
                     /locus_tag="PC700_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2580355.1"
                     /translation="MKRSLIEDLKRNINKIEYDMLTKFYLNLTDLNYILDYHKDLLNT
                     KLEDNSRVYTLYLISLINYIKKDREASYNYLIESLKYIKKLSLKEDSDIISKIYIYLT
                     LSAISIRKYDKANNFYFSAKKVIRQNHLNELLVLLNMSLCVEISALYQTKTDVIALVE
                     EVSFFKTLKNKALVSRANYIFGRVYLYLFNNFIKSMEYLIECLKLAQENNLYSIEAFC
                     KCIISLCYLESGNNVEAIKYINNVLDVAHSKNSISVTERVSIKIDLIWAYLKEDMNKE
                     AEVILLKTIKLMKVVEGVYKDYLYSFIFLYSAEIELRKDNCSFEKVNTYLILSKNIYK
                     SFGDVFIYNSKLDYIYKLYGDLYIKFGNIDEGISCYIKGLNLVKKSSLNLRKISIFYG
                     LIANGYELKKDFKLAIEYYKSMDWYSDRWEKNNSYKTSQAIHKQYELRQKQESLRLLV
                     DDNKKLENDILKDGLTKLYNRRYLEQQLDLFNGTRDKNMVAILVDIDCFKSYNDNYGH
                     LAGDKVIVKVTDIIRSVFIDITEHIIRYGGEEILILVDSIESIGVEEIDNIELYIKRV
                     FKLLEFEGIEHIYSKIKNYITISAGVSIKRCRSRKDVEILIEDADKNLYKSKKAGRNQ
                     YIII"
     gene            3243..5243
                     /locus_tag="PC700_001036"
     CDS             3243..5243
                     /locus_tag="PC700_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2580356.1"
                     /translation="MILLRKKIGIIASDIELKERIEELYREDVENGTIIIDILNLDLM
                     ENQGRILVEKGAQAIIGRGGGYSLVIDTVNVPVIPMNMKSTDLLRAIEIAKKYSKKVV
                     LILGDNEVSFDYVGWRNVISTEITEEWFESKYEIRSKVVKYIDQKDEVVIVGGGLACS
                     FARQYGIDSVFATASDESIREAVEYCKKLLDTLGEEKFNNEVLRNILDGIKDGVIAID
                     SNGSIILYNESAKNMLKVERKCALNKYILDVFPKMEWMLDCLHEKEDVEDRKIRNINN
                     LIVNTRTTLIKVDNSTYGVLGIIQDITKLQNLERKIRFDLNQKGLYARYTFDDFLFKD
                     KLTREFIEEAKKIGKSDYTTLLYGESGSGKEIIAHSIHNISKRKDRPFVAINCATIAE
                     NLLESELFGYEEGAFTGARKGGKRGLFELAHGGTLFLDEINSLSFNIQTKLLRVIEER
                     QIMRIGSDYIIPLDIRIIAATNESLTEKIVMGTFRADLFYRLSSLEINIPPLRDRRED
                     IIPLFNNFVNEVLKDDGLNGINSIDENFVLTKDEMDKLYNYSWPGNVRELKTIAQKYV
                     VTGKIKLRQDRDFKTKKLLPNSEVDKFNSETTASVEVQDESINISKINDGKISIDIKE
                     VNKYVEEKIISMLFAQGLSKNEVAQVLGISRTSLWKKYNKNI"
     gene            5505..6764
                     /locus_tag="PC700_001037"
     CDS             5505..6764
                     /locus_tag="PC700_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2580357.1"
                     /translation="MSQSMVGKHAMWPKENDVIFSISGRAQAAEKAFGMDNVINATIG
                     ALMDDSGKLITMKTVYEEYKALDNCEIGAYAALEGQPDYLEAVKKVFFRDYLPEGHIR
                     VLASPGGSGAIKLAVWNYTNEGDEVLTSDWFWSPYVSIAEEANRKVVNYQLFDENRRF
                     NFESFKEKFVNIAEKQGRVFTIINTPAHNPTGYSVADDEWDKILDLSKEVAKDKDKKI
                     IFFVDSAYIDFAGDDDVCRKFFKKFSNLPENVLVLVGFSMSKGFTAYGMRMGAIICIS
                     SSEDVAEEFHYSCVHSCRANWSNCNRSAMAVLSNIVNDPKKFKEYEDEKEIYKNMLTR
                     RADVFVEEAERVGLEILPYIAGFFVSIPCDNPKEVCEELTKHNLFAVPLKMGLRFAVC
                     AVSEDKCKKAPSIIKEALESLEVKINN"
     gene            6825..8612
                     /gene="iorA"
                     /locus_tag="PC700_001038"
     CDS             6825..8612
                     /gene="iorA"
                     /locus_tag="PC700_001038"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HDF2580358.1"
                     /translation="MKQLMTGNEAIARGAYEAGVKYASAYPGTPSTEILENIATYKDA
                     IVAEWAPNEKVALEAAIGGSIAGARTMASMKHVGLNVAADPIFTYAYTGVNGGMVLIT
                     ADEPGMHSSQNEQDNRMYAKFAKIPLFEPSTSQEAKDMIKEAFEVSEKYDTPVLYRVT
                     TRLCHSKGLVECYDREEVEIKEYVKNAKKMVTVPANAQIRRGVVEERMEILKKFSNET
                     DLNYYEMNDTKIGVIASGMCCNFAKEVFGKNASYMKLGFTNPLPYEKIKEFAEKVDKI
                     YVIEENDPFIEEQIKAYGIDCIGKDVIPPYGEMTPDVLRKAIFGKTNDTIEYKSELVT
                     PRPPSFCAGCPHRGFFYELGKRKNLIVGGDIGCYTLGFAPPYNGIDYVVCMGSAFGTA
                     HGAQKVLNMKDDNEKRLVGVLGDSTFFHTGINGLLDVVYNRGNSISVILDNRITGMTG
                     HQENPGSGYTLQGAKTKEVDIEGLVKACGIEHVRVINPNNLKEVNEALDWALAIEDEP
                     SVIITRWPCVLKKFSKEDIEEFNNPFKTKCKVDHDKCIGCKLCLKTGCPALSFDKENK
                     LSNIDRNQCVGCGVCAQVCPKQAIVKEEK"
     gene            8614..9189
                     /locus_tag="PC700_001039"
     CDS             8614..9189
                     /locus_tag="PC700_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HDF2580359.1"
                     /translation="MTKSILLVGVGGQGTILASKLLTMGLMEAGYDVKMSEIHGMSQR
                     GGSVSSQVRYGDCVHSPVIEIGGADILVSFEKMEALRWFNYLKPEGKALVNNHRIDSM
                     TVLIGGAEYQESDIDAELSRLNAKVINAADKAEELGNSKIMNVILLGCLVKSMQLESI
                     DWEKIISENVKPKFVELNIKAFHEGMEMVSK"
     gene            9242..10312
                     /gene="buk"
                     /locus_tag="PC700_001040"
     CDS             9242..10312
                     /gene="buk"
                     /locus_tag="PC700_001040"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HDF2580360.1"
                     /translation="MTYRILAINPGSTSTKIAVYDGEEQILVKTIDHPAEEIAKYNTI
                     QDQFEMRKEAVLNILKENSIDLKSLSAIVGRGGVLPPVKSGAYLVNEEMIDVLRHRPV
                     LEHASNLGAVVAHAISEPLGINSYIYDSVAVDELIDVARISGLCGMDRSSAGHALNTR
                     AMALKYAKDKGKDYKSLNLIVAHIGGGVSIYLHEKGRMVDMLSDDEGPFSPERSGRVP
                     ATKLVAACYSGQYSEREMTKKIRGKGGIVSYLNTVDAREVEKMIAEGNEEAKIIYEAM
                     AYQLAKGIGELATVVDGKVDAIIITGGIAYSEMFTSMVKKKVEFIAPVEIMAGENELE
                     SLAFGTLRVLNGEEEARIYSEN"
     gene            10530..11231
                     /locus_tag="PC700_001041"
     CDS             10530..11231
                     /locus_tag="PC700_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580361.1"
                     /translation="MGKISLKSMIRLFSGFFIFAISSVLMINAHVGLMPWDVLHQGLS
                     IKLGITIGQASIMVGVVIVILDAVFGENIGWGTLLNMTFIGIFIDLVIFSGVIPHASN
                     TYIGVFMVVIGIILAAIASFLYLGVCLGSGPRDGLMIALQKKTNKSVRLVRTILEILA
                     LVVGWLLGGSVGIGTLVSALGLGYVLQIVFRIFKFDTKLLKHRFIIDDIREWKEKRSN
                     EHKCKSSIVIKNEQN"
     gene            11290..11796
                     /locus_tag="PC700_001042"
     CDS             11290..11796
                     /locus_tag="PC700_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDF2580362.1"
                     /translation="MELWDLYNADGIKTGNVIKRGNSIEEGYYHLAVEVWILNSNSQI
                     LIQKRSKSKKTLPNMWGMTTGCIVSGEDSLEGAIREAKEEIGIDITKDEMKVFRSMIH
                     EDTLWDVYLVKKEYDISKAILQEEEVSDIKWVSTDEIRQLLKEGLFFEYPEMYELLYE
                     IDNNKLNI"
     gene            11971..12993
                     /locus_tag="PC700_001043"
     CDS             11971..12993
                     /locus_tag="PC700_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDF2580363.1"
                     /translation="MEDNKGLARDSSKYKIVSISVVVIILITFWYMLNMVLLTFIMTF
                     IFYNLLVATRKRIKKFSSLNIPDSLIIIVLYALFAILLVLISYAVVPIIIVQLTELSR
                     VFSDFDVNQFAQSLGPKLYPIVSKLDFNKYISQAGLLIASTATKVGSFGVNILLAFLL
                     SLLLLLEKNEIKNFGDKLSDSKISFIYNSLVFFGKSFVKNFGEVMKVQVMIAFINSVV
                     SMIFLGFMGFPQIWALGFMIFVLGLIPVAGVIVSLIPLTVIAFNTGGITKVFGVLLMI
                     CIVHAVETYILNPKLMSNRTKLPVCFVFIILLVGEHYLGVWGLLIGVPIFMFLMDILG
                     VKFTSR"
     gene            complement(13140..13448)
                     /locus_tag="PC700_001044"
     CDS             complement(13140..13448)
                     /locus_tag="PC700_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HDF2580364.1"
                     /translation="MKNNNLNCAATNCAYNTSGYCYAGSIKVDGMQATTTENTYCASF
                     EDKYTSGITSRSNETNQVDTDNIHCEAVKCKYNKNELCKAEKVHINERNASCETFEMK
                     "
     gene            complement(13508..13936)
                     /locus_tag="PC700_001045"
     CDS             complement(13508..13936)
                     /locus_tag="PC700_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580365.1"
                     /translation="MNNKLHELEKNLPETSCSFCTHLSFKGPSLDYRYDIRCVISDTK
                     PDFRGCCEYFEPEYTELNTGDLDNLYINFLETCLKVNYNDYINSMYWKLFKERTLLEN
                     NFKCSICGSTENVDVYHVNKNLGRETSKDVVVKCDKCNCK"
     gene            complement(14159..15616)
                     /locus_tag="PC700_001046"
     CDS             complement(14159..15616)
                     /locus_tag="PC700_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HDF2580366.1"
                     /translation="MTTFLIGLAILLIGGALYGAYCEKVFGPDDRKTPALAQSDGVDY
                     VPMKKWKNSLIELLNIAGTGPILGPIQGILFGPIAFILIPIGCVFGGALHDYMSGMIS
                     IREKGAQMPSLISRFLGNKVFQVYNIFLCLLMLLVGAVFIYTPGDLVVTQILNMKSTI
                     NNPVVWIVYGLIFLYYLCATLFPIDKIIGKVYPIFGAILLLSAAGVGIGIFTQGYDLA
                     NLSLANWKGIHPDGIPLIPTFFVTVACGIVSGFHSTQATLIARSVSNEKEGKTTFYNM
                     MILEGLIAMIWAAAAMGIYNKGIPKELVGSPDVIGLVARDLLGSIGGIIAIIGVIVLP
                     ITSGDTALRSLRLMLADYFHYDQKEKKHRVILSICIFIPVIAILIFAKLSASGFNILW
                     RYFSWSNQTIAIFAFAMITVYLIIKEKNYIISLIPGMFYSFVIFSYIFNAQIGFNLNI
                     NISYVLAAIFTVLYAILTVRSGRKLKSKADTKLAD"
     gene            16034..16948
                     /gene="nadA"
                     /locus_tag="PC700_001047"
     CDS             16034..16948
                     /gene="nadA"
                     /locus_tag="PC700_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HDF2580367.1"
                     /translation="MDKDLTYQIKELKKEKNAIILAHFYQPPEIQELADAVGDSYYLS
                     EIARDCKEEVVVFCGVRFMGESAKLLSPEKTVLMPVSNAGCAMADMVDEEGVIKLKQQ
                     YPNALVVCYINSTAKVKAHCDVSVTSSSAIKILENIDNKEIIFLPDKNLGGYIAEQFP
                     DKNFIFWDGYCKYHNNIRAEEIIELKDKYKNAEVLVHPECKKEIRDLGDYVGSTSGII
                     KYATNSKNKDFIIATEEGILHELKKNNPNKNFYIPGGKILCTDMKKTTLENLYSTLKN
                     MENEVIVEDEIMEKALNSLLNMHKLAEG"
     gene            16961..18262
                     /locus_tag="PC700_001048"
     CDS             16961..18262
                     /locus_tag="PC700_001048"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HDF2580368.1"
                     /translation="MNLEQDVLIVGSGVSGLYCALNLDKSLNVLVVSKSTIENNNTYL
                     AQGGISTARNIDDIESFVEDTMKAGQYKNRVEAVQVLADESIENVGQIVEYGMPLDKE
                     NGEIDYTREGAHSVNRIVHSKDNTGEVVFKTLLKEAKTRENITLIEDAYLLDILKDGN
                     KCIGARIFKSKKEIHVFSKIVVLATGGIGGLFKNSTNQRHLTGDGIAIALRNNIKIEN
                     LEYIQIHPTAFYEENNEGRRMLISESLRGEGAKLLNKNKERFVDELLPRDVVSKAIFE
                     QMEKDKLPYVYLDATHLDSEYLINRFSFIYNECLARGTDITKECIKVSPAQHYFMGGI
                     HVDLDSKTSMENLYAVGEISCTGVHGANRLASNSLLEGLVFSKRAAKNINSVIDNVKV
                     KFIDVPDMDIDIEQVKKENKILVIKEIERTSEDFGDELFDY"
     gene            18243..19079
                     /gene="nadC"
                     /locus_tag="PC700_001049"
     CDS             18243..19079
                     /gene="nadC"
                     /locus_tag="PC700_001049"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HDF2580369.1"
                     /translation="MNYLIIDRMIQDALIEDVPSEDITTNSIVDENSKSTVDLICKQD
                     GIIAGLGVFKRVFEILGDVDVKLYKNDGDRVKNREKIAVLTGSTRNLLVGERVALNYL
                     QRMSGIATITSRYVEKLEGTNTKLLDSRKTIPNLRILDKYSVKVGGGCNHRFNLSDGI
                     LLKDNHIGAAGSVKKAVELARKNTSFVRKIEVEVETLDMVKEAIEANADIIMLDNMSL
                     EMAKQAVDIINGRAIVEFSGNVNLDTIEDIGKIGVDVVSVGALTHSVKALDISMKNLR
                     NI"
     gene            19335..19667
                     /locus_tag="PC700_001050"
     CDS             19335..19667
                     /locus_tag="PC700_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HDF2580370.1"
                     /translation="MKVAYIFSSTNTHKILDKMIIPQLEQGTHGVDVLGMFFFMDNTL
                     FLSKGNPVGERLSKLHEKTDMIIMACDQCAIERGIENNLVDGATIGCFPNLYACLGSA
                     GVDQVITL"
     gene            19829..20014
                     /locus_tag="PC700_001051"
     CDS             19829..20014
                     /locus_tag="PC700_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580371.1"
                     /translation="MSEKYADKLEIKLYQAGKDFSYIKKYGIITKGTLIINQKKKYDR
                     LNKDTIERAIVEAINNN"
     gene            20041..21126
                     /locus_tag="PC700_001052"
     CDS             20041..21126
                     /locus_tag="PC700_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDF2580372.1"
                     /translation="MNILEIVLNGVKDTIVASIVTLNGASGWLMISFILAGVLHNVLT
                     PRRFQKQLGNKKFSSLIKSTISGMFLPICSCGVIPLGISMYYSGAYLGPVLAFMTSTP
                     IINPIAVVLCLGLLGPKVTIIYLIAGFLVPFLVGFLGNCFGKEELSLYNDSEEEVIEL
                     EEEKRSFMENMIDGLKWSFGELALTISKYVVMGMVFAGFITTIFPNSIIQKYLGNPGL
                     LSLGSIAILACVMYVCAVGHIPFIAALVASGASPGIAITFLIAGAATNLPELISMFKL
                     IGKRTVIIYSVTLTLSSIIIGYITNLLLVNNNSVNLDATNSSIQLANKLMLNIPDSFE
                     YVCTILIILFAFKSTIPKMKEVIGNHA"
     gene            21119..21577
                     /locus_tag="PC700_001053"
     CDS             21119..21577
                     /locus_tag="PC700_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580373.1"
                     /translation="MLKKIVIISCIVVVLIILSKIVDDNIKEDASIPNVNKETLEYFR
                     KNYKEDIITCAEEDLNNDGKKDLVVIYKKSNNSNEMVVVVSDKNSHYITKPIPAPIEN
                     QTITFKNIDDKAPIEVIVSGSKNGNVGYAIYRVEGKKFVDLFGEDMDKCC"
     gene            21612..22703
                     /locus_tag="PC700_001054"
     CDS             21612..22703
                     /locus_tag="PC700_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2580374.1"
                     /translation="MKLSKKIQALILFGVMGTSILTGCSSKPKEKEEAKAGKDDYTIT
                     VGYYNCDHMTAGPVAESAGIYKDLGLNVKTVGNGKVPEAMAAGKMDAGYIGTKGLVGA
                     IPKGSPITIAANNHTGGSEYLIVSKDIKEPKDLIGKKIATDMSDFLWTSDYGPETGLP
                     TDPSKYEVVNMDSDKDKYLALKTGKIQAFTSCDPWGSVAENDGAGKIIASTQYKEKAN
                     GKEYNCCSFSLNKNFIKEHPDLAKKLVLAHTKAIEYIYTNPAEAAKIFAKYYNVEEEV
                     ALRTIYKKTVGEGRTLTWKVTGEEYKNNLQMYKDLKALDDIPKYEDTIDTSLLDSCGA
                     DDFDKFIKEKVNPEFPEGMSYEDWKAKVL"
     gene            22708..22932
                     /locus_tag="PC700_001055"
     CDS             22708..22932
                     /locus_tag="PC700_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580375.1"
                     /translation="MENIKNIIILFIIILGSIFIIFFNFEDKEEIKGKLRIGVSDDNS
                     GFVINYMVNRDYFENIKVSDIMDIFTINDC"
     gene            22942..23505
                     /locus_tag="PC700_001056"
     CDS             22942..23505
                     /locus_tag="PC700_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580376.1"
                     /translation="MQWALSSEEIDIAIICKDAAKQYVNVNSGFEIVGTVVQNSDIFL
                     LSDNNPEKIGVIQNRDYQSELVKKYYKNVEVAPLLGTALAYGLESNLVDGVVIDCIKS
                     IGLEGKRKSTTKLGDYDTYVLVVSKEFKKSKAYSDFITLYNKSVDALKNKATFKKALK
                     EYRNIRNIDMEEISNWKLKFLKIKAID"
     gene            23466..24095
                     /locus_tag="PC700_001057"
     CDS             23466..24095
                     /locus_tag="PC700_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2580377.1"
                     /translation="MEIKISKDKSDRLTKKKTDGSMDILYKIILLVIIFLVWHFAAKD
                     IGSSLLLPMPVDVIKGFFFCVTDAETVTNLFITLQRVLKGFMYALLFGLPIGFIMGFS
                     KTFERVLSPVVDSVRQVPIMAWVPLTIVWFGIGDGPTIFLIAFSGVFPIILNTIQGVR
                     AISKDYYNAARSMGASPIVIFTNVIVPASLPDILTGSRIAISTGWMSVI"
     gene            24135..24272
                     /locus_tag="PC700_001058"
     CDS             24135..24272
                     /locus_tag="PC700_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2580378.1"
                     /translation="MVQAQTLMHTDVLIGLMIFAALIGFIIDRVLKFINKILCRWRFA
                     D"
     gene            24272..25024
                     /locus_tag="PC700_001059"
     CDS             24272..25024
                     /locus_tag="PC700_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2580379.1"
                     /translation="MKLSVRDINKTFVNNRVHTKVLEDISMDIDDGQFVCLLGPSGCG
                     KTTLLTIIGGFQKSERGDVFINDKRVKKPGIDRAFIFQNYALFPWKTIRGNVLFPMKQ
                     QKIPKEKREEMLEELLVMSDLKGKEKLFPHQLSGGMKQRVAMIRALACNPEVLLMDEP
                     LGAVDFQMRQNLQEELERIWIKKKITALMVTHDVDEAVYMSDRVIVMSRDKGRIIDDI
                     NIDIPRPRIRGSQKYEEYKNKLTDTLSKCYEV"
     gene            25037..25522
                     /locus_tag="PC700_001060"
     CDS             25037..25522
                     /locus_tag="PC700_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580380.1"
                     /translation="MSQIEKYNSLDNTQQKIRISIMDMIIDKGSSVTLQEVTEYVSKK
                     LNIEKEYIERTLQYFIYENIMVVDGNSINFIYPVSALQTNHKVTLRDNRSFSAMCAID
                     AIGTSCTFNQDIKINSICSATGKEIEVIIKNEKIEYVNNPNLRVLHINLDKHLNWAAS
                     C"
     gene            25529..25678
                     /locus_tag="PC700_001061"
     CDS             25529..25678
                     /locus_tag="PC700_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580381.1"
                     /translation="MNFFWTKSDFDAWTNEAGLSDDEDIYCLDINEAIVESYKIFKLK
                     QKVLS"
     gene            complement(25814..26635)
                     /gene="yidA"
                     /locus_tag="PC700_001062"
     CDS             complement(25814..26635)
                     /gene="yidA"
                     /locus_tag="PC700_001062"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HDF2580382.1"
                     /translation="MYKLIALDIDGTILNTQKRITPEVFESIQEAKRAGAKVVITTGR
                     PLPGVKELLNQLNLTDEGDYVICFNGAIIQEVKSEKIIHDVEMTLDDFDFIYNNVCKK
                     YKTKIHINTMTNLITPNEAPGKYTLHEAKLNNIEVKYIQKDKIDESIKICKIMIVDEP
                     ERLEEIIQQLPKNLFNKYTIVRSAPFYLEFLGKTTNKGTALKTLCTNLNIPIENAIAV
                     GDEENDQHMIKYAGLGVAMGNARNSIKEIADYVTDTNNENGVAKVINKYILNKAI"
BASE COUNT        10580 a         2833 c         4797 g         8774 t
ORIGIN      
        1 aaaaaatata tttttttatt attggtaaaa tatatttttt gtttttggat gatgctctaa
       61 ttgctaaagt aaatttttta aaagcaagaa aatatgcaga tttactaaag gatatgcaag
      121 tatcttcact atgtaatata aagataggtg agtgtgaatg ttcatttgaa aattatgaat
      181 gttccataga atatttaaat gaagtaataa gcaataaaaa gtatagaaat gtaaatataa
      241 ttcaaaaata tagagcatca aacaacataa ctaaaatatt aataaagact agaaattatc
      301 ctgtagcaat aaataattta gcaaaatctg aagtatatct agaaaaaatt aaaaatcaga
      361 gcttaaaaga agtagagaag tttgatttat ttataagttt agctatgtat tatgcaaaaa
      421 gtaatgaaaa atcttttgaa caatcaatat gctatttaga tagagctaaa aacatcttaa
      481 atatgataaa aaatatagaa gaatatacaa tatatgattt agaaataatt tatcatcagg
      541 caaagatata ttatatcttt ggatattatg acaatgcctt aaacacaaat aaaaaactcc
      601 ttgaaaaatc taaaaatgct aaaaattata attatgtgaa gttagcatac aagaatatat
      661 atttgtgttt tgaaaagatt caaaattatg aaatgagtat gaaatacttt aaaatgtatt
      721 atgagttaaa aatgatgtat ataaaagctc gaaatagaaa ttatatagat tccttaaatt
      781 atagacatga gtatatagaa acagaaataa ataacatgag aaaagtaaaa cttgatttag
      841 ataaaaaaaa atatgttgac catttgacaa gtgcatataa tagaactttt ttgaacgatt
      901 ttttagaaaa acaagaagtt agtgaatatt gcacagcatt tatgatagat gtagattact
      961 ttaaagaata caatgatact tatggacact ataatggaga tattgctctt aaaaatataa
     1021 ctataataat aaaaaaatat ctaaaaaaag acatgtttat gataaggtat ggtggagaag
     1081 aatttctgat attatctgta tgtaaagatt ataaaaaaag caaaatattt ggaagaaaat
     1141 tatgtaggat tgtaaagaaa tttttaaaca ataatttgac agttagtatt ggcattgata
     1201 catgtaaaaa tagtagtata gatataagag aaataattga aaatgctgat aaagctttat
     1261 ataaagcaaa ggaaagtggt agaaatagat gtttgcatta tcatgatttt aagaattttt
     1321 aactgttgaa ataggattgg tgatttactt gaaaaggagt ttaatagaag atttaaagag
     1381 aaatataaac aagatagaat acgatatgtt gactaaattc tatctaaatt tgacagattt
     1441 aaactacatc ttggattatc acaaagacct tttaaataca aaactagaag ataatagtag
     1501 agtctatact ttatatttaa ttagccttat aaactatata aaaaaagata gagaagcatc
     1561 atacaactat ttgatagagt cattaaagta tattaaaaaa ttgtcattaa aagaagattc
     1621 agatataata tctaaaattt atatttatct tactttatca gctatttcta taagaaaata
     1681 tgataaggca aataattttt atttctcagc aaaaaaagtt ataagacaaa atcatttaaa
     1741 tgagttgcta gtcttgttaa atatgagttt atgtgtagaa ataagtgctt tatatcagac
     1801 caaaacggat gtaatagctt tagtagaaga agtgtctttt tttaaaactt taaaaaataa
     1861 agctcttgta agtagggcaa attatatatt tgggagagtt tatctatatt tatttaataa
     1921 ctttataaaa agtatggagt acctaattga atgtctaaaa ctggctcaag aaaataattt
     1981 atattctata gaggcttttt gtaaatgtat aataagtttg tgttatttag agagtggaaa
     2041 taatgtagaa gctataaaat acataaataa tgtattagat gttgcgcaca gtaagaactc
     2101 aatatcagta actgaaagag tgtctataaa gatagacctt atatgggcat atttaaaaga
     2161 agatatgaat aaagaagcag aagttatttt attaaagact ataaaattaa tgaaagttgt
     2221 tgaaggtgta tataaggatt atttatattc atttatattt ttatattcag ctgaaattga
     2281 gttaaggaaa gataattgta gttttgaaaa ggtaaatacc tatttaattt tatcaaaaaa
     2341 tatatataaa agttttggcg atgtttttat atataattct aaactagatt acatatataa
     2401 gttgtatgga gatttatata taaaatttgg taatatagat gaaggtataa gttgttatat
     2461 aaaaggttta aatttagtta aaaagagttc attgaatctt agaaaaatat ctatatttta
     2521 tgggctaata gcaaatggat atgagctaaa aaaagatttt aaactggcaa tagaatatta
     2581 taaaagtatg gattggtatt ctgataggtg ggaaaaaaat aattcctata aaacatctca
     2641 agcaattcac aaacaatatg aactcagaca aaaacaagag agtttaagat tactagtgga
     2701 tgataataag aaattagaaa atgatatttt aaaagatgga cttactaaac tatataatag
     2761 aagatatttg gaacagcagt tggatttatt taatggaacc agagacaaaa atatggttgc
     2821 aatactagtc gatatagatt gttttaaaag ttataatgat aattatggtc atctagcagg
     2881 agataaagtt atagttaagg taactgatat tataagaagt gtatttatag atattaccga
     2941 acatataatc agatatgggg gagaagaaat tttaatacta gtagattcta tagaatcaat
     3001 cggtgttgaa gagattgata atatagaatt atatattaaa agagttttca aacttcttga
     3061 atttgagggt attgaacata tatattctaa aataaaaaac tatataacta taagtgctgg
     3121 agtaagtata aaaagatgtc gtagtagaaa agatgttgaa atattaattg aagatgcaga
     3181 taagaaccta tataaatcaa aaaaagcagg tagaaatcaa tacattataa tttaaaattg
     3241 aggtgatttt gttgcgaaaa aaaataggaa ttatagcctc tgatattgaa ttaaaagaaa
     3301 gaatagaaga attatataga gaagatgtag aaaatggaac tataataatc gacatactta
     3361 acctagactt aatggaaaat caaggtcgta tacttgttga aaaaggtgcc caagctataa
     3421 ttggtagagg tggaggttat agtttagtaa tagatacagt aaatgtacct gttataccaa
     3481 tgaatatgaa gtcaactgac cttttaagag caatagaaat agcaaagaaa tattctaaaa
     3541 aagtagtatt gattcttgga gacaatgaag tttcttttga ctatgttggc tggagaaatg
     3601 tcataagtac tgaaataact gaagaatggt ttgaatctaa gtatgagata agaagtaaag
     3661 ttgtaaaata tatagaccaa aaagatgaag ttgtaatcgt aggaggaggt ttggcatgtt
     3721 cttttgcaag gcaatatgga attgatagtg tatttgcaac tgctagtgat gaatctataa
     3781 gagaagctgt agaatattgt aaaaaattat tggatacatt aggtgaagaa aagtttaata
     3841 atgaagtgtt acgaaatata cttgatggta taaaagatgg agtaattgct atagatagta
     3901 atggtagtat aattttgtac aatgaaagtg caaaaaacat gcttaaagta gagagaaaat
     3961 gtgcacttaa taaatacatc ttagatgtat ttcctaaaat ggagtggatg ttagattgtt
     4021 tacatgaaaa agaggatgta gaagatagaa agataagaaa tataaataat ctaattgtta
     4081 atactaggac aacattaata aaagttgata atagtactta tggagtttta ggaataatac
     4141 aagatataac caagctacaa aacttagaaa gaaaaataag atttgacttg aatcaaaaag
     4201 gtctctatgc tagatatact tttgatgatt ttttatttaa agataaatta actagagaat
     4261 ttattgaaga agctaaaaaa attggaaaaa gtgattatac aactttactt tatggagaaa
     4321 gtgggtctgg taaagaaatt atagctcata gtatacacaa tataagtaaa agaaaagata
     4381 ggccttttgt tgctataaat tgtgctacta tagcagaaaa tttacttgaa agtgaacttt
     4441 ttggctatga agaaggtgca tttactggtg ctagaaaggg tggaaaaaga ggactatttg
     4501 aacttgctca tggagggact ctttttttag atgaaataaa tagtctatcc tttaatattc
     4561 agacaaaact attaagagta atagaggaac gtcaaataat gagaattggt tctgattata
     4621 taatacctct ggatataagg attatagcag caacgaacga atctttaacc gaaaaaatag
     4681 ttatgggtac atttagagca gacttatttt atagattgag tagtttagag ataaatatac
     4741 ctcctttaag agacagaaga gaagatataa tacctttgtt taacaatttt gtaaatgaag
     4801 tattaaagga tgatggtctc aatggaataa acagtataga tgaaaatttt gttttgacta
     4861 aagatgaaat ggataagcta tataattata gctggcctgg aaatgtaaga gagttaaaga
     4921 caatagctca aaaatatgtt gtgacaggta aaattaaatt gagacaagat agagatttta
     4981 aaactaaaaa attacttcct aactctgaag tagacaaatt taattctgaa acgacagcaa
     5041 gtgtagaagt ccaagatgaa tctataaata taagtaagat aaatgatggt aagataagta
     5101 tagatataaa agaagtaaat aagtatgttg aagaaaaaat aataagtatg ctttttgctc
     5161 agggactttc taaaaatgaa gttgcacaag ttcttggaat aagcaggaca tctttatgga
     5221 agaaatacaa taaaaatatt taaatataat tacatataaa ttattttgaa gtaatgttaa
     5281 ttttttaaaa caagtgttaa gaaaaattaa cattactttt ttgtaaattt ttattttttt
     5341 agacaggcat atatatttat aagaaaaata gttaaaacac ttaatttaat aatgaaaaaa
     5401 ataataattc taaacaaaat caattttaca taaagtggga tttattggca tagtaattgc
     5461 ttaatatata atgtaagagt aaatacaatt ttaggggtga tggtatgagt caaagtatgg
     5521 ttggtaagca tgcaatgtgg ccaaaagaaa atgatgttat atttagcata tctggtagag
     5581 ctcaagcagc agagaaggct tttggtatgg ataatgtaat aaatgcaaca ataggcgctc
     5641 taatggatga ttcaggaaaa ttaataacaa tgaaaactgt atatgaagaa tataaagcat
     5701 tagataactg tgagataggt gcatatgcag ctcttgaagg tcaaccagac tacttagaag
     5761 cagttaaaaa agtatttttc agagattact taccagaagg acatataaga gtattagctt
     5821 caccaggagg aagtggtgca ataaaattag ctgtatggaa ctatactaat gaaggagatg
     5881 aggtattaac atctgattgg ttttggagtc catatgtaag tatagctgaa gaggctaata
     5941 gaaaagtagt taattaccaa ctttttgatg aaaatagaag gtttaatttt gagtcgttca
     6001 aagaaaaatt tgtaaatata gcagaaaaac aaggtagagt ctttacaatt ataaacacac
     6061 cagcgcataa tccaactgga tatagtgttg cagatgatga gtgggataaa atattagact
     6121 tatcaaaaga agtcgcaaaa gataaagaca aaaagataat atttttcgta gattcagcat
     6181 atattgactt tgcaggtgat gatgatgtat gtagaaagtt ttttaagaaa ttttctaatt
     6241 tgcctgaaaa cgttctcgta ttggttggat tcagtatgtc aaaaggattt acagcttatg
     6301 gtatgagaat gggggctata atttgtatat cttcaagtga agatgtagct gaagagttcc
     6361 attactcatg tgttcattca tgtagagcaa attggtcaaa ttgtaataga agtgccatgg
     6421 cagtattatc taacattgta aatgacccta aaaaattcaa agaatatgag gatgaaaaag
     6481 aaatatacaa aaacatgctt actagaagag cagatgtatt tgtagaagaa gctgaaagag
     6541 taggattaga aattcttcca tatatagcag gtttctttgt aagtatacca tgtgataatc
     6601 caaaagaagt atgtgaagaa ttgactaaac ataatttatt tgcagttcca ttaaaaatgg
     6661 gcttaagatt tgcagtttgt gcagtttctg aagataaatg taagaaagca ccaagtatta
     6721 taaaagaagc acttgaaagt ttagaagtaa aaataaataa ttaatttcat tgaacaaatt
     6781 gggtggacta tgaatttaat aattgtagat ggagggaatt ttatatgaaa caattgatga
     6841 caggtaatga agcaattgca cgtggagcct atgaggctgg tgttaaatat gcatctgcat
     6901 atccagggac tccaagtaca gaaatattag aaaatatagc aacatataaa gatgcaatag
     6961 ttgcagagtg ggctccaaac gaaaaggtag cacttgaggc tgctataggg ggttcaattg
     7021 caggagcaag aacaatggct tctatgaaac atgttggttt gaatgttgca gctgatccaa
     7081 tatttactta tgcatataca ggggtaaatg gtggtatggt acttattaca gcagatgagc
     7141 cgggaatgca ttcatctcaa aatgagcaag ataataggat gtatgcaaaa tttgcaaaga
     7201 tacctttatt tgaaccatca actagtcaag aagctaaaga tatgataaaa gaagcttttg
     7261 aagttagtga aaaatacgat acaccagttt tatatagagt tacaacaaga ctttgtcatt
     7321 caaaaggatt agtggagtgc tatgatagag aagaagttga aattaaagag tatgttaaaa
     7381 atgctaagaa aatggtaact gttccagcaa atgctcaaat aagaaggggc gttgttgaag
     7441 aaagaatgga aatccttaaa aaattctcaa atgaaactga tttaaactac tatgaaatga
     7501 atgatacaaa aataggtgtt atagcttctg gtatgtgttg taattttgcc aaagaagttt
     7561 ttggaaaaaa tgcatcatat atgaaactag gatttacaaa tccattacct tatgaaaaaa
     7621 taaaagaatt tgcagaaaaa gttgataaaa tatatgttat agaagaaaat gacccattta
     7681 tagaagaaca aataaaagca tatggaatag attgtattgg aaaagatgta ataccaccat
     7741 atggagaaat gacaccagat gttttaagaa aagctatatt tggaaaaaca aatgatacta
     7801 tagaatacaa atctgaatta gtaacaccaa gaccaccaag tttctgtgca ggatgtccac
     7861 atagaggatt cttctatgaa cttggaaaga gaaaaaattt aatagttggt ggagatatag
     7921 gttgttatac tcttggattt gctcctccat ataatggaat agattatgtt gtttgtatgg
     7981 gttctgcttt tggaactgca catggagctc aaaaagttct taatatgaag gatgataatg
     8041 aaaaaagatt ggtaggtgta cttggagact caacattctt ccatacagga ataaatggac
     8101 ttttagatgt tgtttacaat agaggtaatt ctatttcggt tatattagat aatagaataa
     8161 ctggaatgac aggtcaccaa gaaaacccag gttcagggta tactttacaa ggtgctaaaa
     8221 ctaaagaagt tgatatagaa ggattagtta aagcttgtgg aatagaacat gtaagagtta
     8281 taaaccctaa caatttaaaa gaagtaaatg aagcactaga ttgggcttta gctatagaag
     8341 atgaaccatc agtaataata acaagatggc catgtgtact taaaaaattc tctaaagaag
     8401 atatagaaga atttaataat ccatttaaga ctaagtgtaa agttgaccat gataaatgta
     8461 taggatgtaa attatgtttg aaaactggat gtccagctct atcatttgat aaagagaata
     8521 agttatcaaa tatagataga aatcagtgtg taggatgtgg agtatgtgca caagtttgtc
     8581 caaaacaagc aatagttaag gaggaaaaat aatatgacta agagtatact tttggtaggt
     8641 gtaggaggtc aaggaactat acttgctagt aaattattaa ctatgggatt gatggaagct
     8701 ggatatgatg taaagatgag tgaaatacat ggaatgagtc aaagaggagg ttcagtttcc
     8761 tcacaagtta gatatggaga ttgtgttcat tctccagtta tagaaattgg tggagcagat
     8821 atattagtat cttttgaaaa aatggaagct ttaagatggt tcaattattt aaaaccagaa
     8881 ggaaaagctt tagtaaataa tcataggata gattctatga ctgttttaat cggaggagct
     8941 gaatatcaag aaagtgatat agatgcagag ttaagtagat taaatgcaaa agttataaat
     9001 gcagctgata aagctgaaga actaggaaat tcaaaaataa tgaatgttat tttattagga
     9061 tgtctagtta aatctatgca attagagtct attgattggg aaaaaattat aagtgaaaat
     9121 gttaaaccaa aatttgttga attaaacata aaagctttcc atgaaggtat ggaaatggtt
     9181 agcaaatagt ttataataga tataaataga ctatagaaat taaggtgaaa ggaagaaaat
     9241 tatgacttac agaatattag ccataaatcc aggttctact tctacaaaaa tagcagtata
     9301 tgatggagaa gaacaaattc ttgtgaagac gatagaccat ccagctgaag agattgcaaa
     9361 atataatact atacaagacc agtttgaaat gcgtaaggaa gcagttttga atattcttaa
     9421 agaaaatagt atagacttaa aatctcttag tgcaatagta ggaagaggtg gagttttacc
     9481 accagtaaaa tcaggagcat atctagtaaa tgaagaaatg attgatgtac taagacatag
     9541 accagtactt gaacacgctt ccaatttagg tgctgttgtg gcacatgcaa tatcagaacc
     9601 tcttggaatc aactcatata tttatgattc tgttgcagta gatgagctta tagatgtagc
     9661 gagaatatct ggactttgtg gaatggatag atcaagtgca gggcatgcat taaatactag
     9721 agcaatggct ttaaaatatg ctaaggataa aggaaaagat tataagagct taaacttaat
     9781 agtagctcac attggtggag gagtaagtat ttatcttcat gaaaaaggaa gaatggttga
     9841 tatgctatct gatgatgaag gaccattttc tccagaaagg tcaggaagag tacctgctac
     9901 aaaattagtg gctgcctgtt attcaggtca atattcagaa agagaaatga ctaaaaagat
     9961 aagaggtaaa ggtggtatag tttcatacct aaatactgta gatgctagag aagttgaaaa
    10021 aatgatagca gaaggaaatg aagaagcaaa aattatttat gaagcaatgg cttatcagtt
    10081 agcaaaaggt attggagagt tagcaactgt agtagatgga aaggtagatg ctataattat
    10141 aacaggtgga attgcatatt ctgaaatgtt tacttcaatg gttaaaaaga aagttgagtt
    10201 tatagcacca gtagaaatta tggcaggaga aaatgagttg gaatcacttg cttttggaac
    10261 tttaagagta ctaaatggag aagaagaagc tagaatttat agtgaaaatt aataagaaaa
    10321 attaaaaatg tttatatact aaaaaagtgg tatatccgtt ttttatacac gaaagcttag
    10381 agtataggtg aaaatgtata aaatttggat tgccactttt tataattaaa attttatctt
    10441 aatctaataa ataattctgt aaaatttcgt atttatatat atattttatt ggtatttatt
    10501 gttattatta ttatgaggag tgatttttga tgggaaaaat aagtttaaaa agtatgataa
    10561 gattattttc aggttttttt atatttgcaa tttcttctgt gcttatgatt aatgcacatg
    10621 taggtcttat gccttgggat gtacttcacc aaggtttatc tataaagcta ggcataacta
    10681 taggtcaggc ttctataatg gtaggagttg taattgttat attggatgca gtttttggtg
    10741 aaaatatagg atggggtact ttactcaaca tgacatttat aggaattttt attgatttag
    10801 taatattttc tggagtgata cctcatgcaa gcaacactta tataggtgtg tttatggttg
    10861 ttataggaat tattttggca gctatagcaa gttttctata cttaggtgtt tgtttaggaa
    10921 gtggaccgag agatgggctt atgattgctc ttcagaaaaa aactaataaa tctgtacgtc
    10981 ttgttagaac aattcttgaa atactagcat tagttgttgg gtggttatta ggaggttctg
    11041 taggtattgg tacgcttgta agtgctttag gtttaggata tgtattacaa atagtgttta
    11101 gaatatttaa atttgacaca aagttgctga aacatagatt tattatagat gatataagag
    11161 aatggaagga aaagagaagc aatgaacata aatgtaaatc aagtattgta ataaaaaatg
    11221 agcagaatta attatgctaa aatatatttg tagtttatct aatttatagt aaagtcagga
    11281 gattttgaaa tggaattatg ggatttatat aatgctgatg gaattaaaac aggaaatgtt
    11341 ataaaaagag gtaattctat tgaagaaggt tattaccatt tggcagttga agtgtggatt
    11401 ttaaatagta actctcaaat tttaattcag aaaaggtcta aaagtaaaaa gacattgcca
    11461 aatatgtggg gaatgacaac tggatgtatt gtttctgggg aagatagttt agaaggagca
    11521 attcgcgaag caaaggaaga aatagggata gatataacta aagatgagat gaaagttttt
    11581 agaagtatga ttcatgaaga tactctttgg gatgtatact tagttaaaaa agaatatgat
    11641 atatccaaag ctattttaca ggaggaagaa gtaagtgata taaagtgggt aagcacagat
    11701 gaaataagac aattactaaa agagggtttg ttttttgaat atccagagat gtacgaattg
    11761 ctatatgaga tagataataa taaattaaat atatgatgta gagcagattt cagtataagt
    11821 aagattattt aaaatgtatc ttctctaaat tttaatattg ttttatgaaa attaataagt
    11881 tatttttagt atttttctac ttgttttttg agattaaaat aaaaagggca gtcattataa
    11941 agtcataaaa atttggggag gatttaaact atggaagata acaaaggatt ggcacgtgat
    12001 agttcaaaat ataaaattgt ttctatatct gtcgtagtaa ttattctaat tacattttgg
    12061 tatatgttaa atatggtgct tttaacattt attatgactt ttattttcta taacttgctt
    12121 gtagccacaa gaaaacgaat taagaagttt tcttctttaa atattccaga ttcattgata
    12181 attatagtgt tatatgcact atttgctata ttgttagtat tgattagcta tgcagttgta
    12241 ccaataatta tagttcaact tacagagctg agtagggttt ttagtgattt tgatgtaaat
    12301 caatttgctc aatctttagg accaaaattg tatcctattg tctcaaagct ggattttaat
    12361 aaatatattt cacaagcagg gctacttata gcatcaacag caacaaaagt tggaagtttt
    12421 ggagtaaata tattattggc atttttactg agtttactcc ttttacttga aaaaaatgaa
    12481 attaaaaatt ttggagataa actttctgat agtaagattt cttttattta taattcgctt
    12541 gtattttttg gaaagagctt tgttaaaaat tttggtgaag taatgaaagt acaagttatg
    12601 attgccttta taaattctgt tgtttctatg attttcttag gtttcatggg attccctcaa
    12661 atatgggctt taggatttat gatttttgtg ttgggtctta ttccagtagc aggagttata
    12721 gtgtctttga ttccattaac tgtaatagca tttaatacag gaggaataac aaaggttttt
    12781 ggagttttac ttatgatttg tatagtgcat gcagtagaaa catacatatt aaaccctaag
    12841 ttgatgtcga atagaactaa acttccagtt tgttttgttt ttattatttt acttgtagga
    12901 gaacattatt taggtgtatg gggattacta attggagttc caatatttat gttccttatg
    12961 gatattctgg gtgttaaatt tacttctagg taatatatta aaataataat ttatttttat
    13021 atataaatta tgaattgttt taaaaaagaa cctataaata gacatcatta ataaagacat
    13081 ctatttgata ggttcaattt tgctttgtat aaatactata gaattaaata aaaagaaatt
    13141 tatttcattt caaaagtttc acaacttgca tttctttcat ttatatgaac tttttcagct
    13201 ttgcaaagtt catttttatt gtatttacat tttacagctt cacaatgaat gttatctgta
    13261 tcaacttggt tagtttcatt agaacgacta gttataccag atgtatattt gtcttcaaaa
    13321 gaagcacaat aagtattttc tgtagtagtt gcttgcattc catctacttt tatactacca
    13381 gcataacaat atccactagt attataagca caattagtag cagcacaatt taaattatta
    13441 tttttcataa ataacctcct aagttttatt taaacttatt atgtgaagtt atctaaattt
    13501 tatacactta tttacaatta catttatcac atttaacaac tacatctttg gaagtttccc
    13561 ttcctaaatt tttattaaca tgatacacat caacattttc agttgatcca catatagaac
    13621 atttaaaatt gttttctagt agggttcttt ctttaaataa cttccaatac atagaattga
    13681 tgtaatcatt ataatttacc ttgaggcatg tctcaagaaa gtttatgtat agattgtcta
    13741 aatctcctgt atttaattct gtatattctg gttcaaaata ttcacaacat cctctaaaat
    13801 caggctttgt atctgaaata acacatctaa tatcatatct atagtctagg ctaggcccct
    13861 taaatgaaag atgtgtgcaa aaagaacaag atgtttctgg tagatttttt tcaagttcat
    13921 gtaatttatt attcataagt attcctccaa aaaatttatt ttaagtatat aaatgaatag
    13981 attattttaa aacttttatt tatttatatt agagatttag gataaatatt ccaataagga
    14041 gtaaatactt aagtatttat tactgtatta aataaaaaag aggcaaatct aacttgagat
    14101 tgcctacttt tttatcattt tttaataaaa tttattctaa gtattatttt atttagattt
    14161 agtcagccaa tttagtatcc gctttgcttt ttaactttct accagagcgt acagttaaaa
    14221 tagcatataa tacagtaaat atggcagcta aaacatatga tatatttatg tttaagttaa
    14281 atccaatttg agcattaaat atataactga atataacaaa agaataaaac ataccaggaa
    14341 ttaagcttat aatataattc ttttctttta ttataaggta aactgtaatc attgcaaatg
    14401 caaatatggc aatagtttga ttactccaag agaagtatct ccacaatata ttaaatccag
    14461 aagcactaag ctttgcaaat ataagaatgg ctatgacagg aataaatata cagattgaca
    14521 atataacacg atgtttcttt tccttttggt cataatgaaa gtaatctgca agcattaatc
    14581 ttaaagatct taaagctgta tctccagaag ttattggtaa aacaattaca cctattatag
    14641 ctatgatacc accaatagaa ccaagtaaat ctcttgcaac caaacctatt acatcaggag
    14701 aacctactaa ttccttaggt attcctttat tgtaaatacc catagcagct gcagcccaaa
    14761 tcattgcaat taaaccttct aaaatcatca tattataaaa agtagttttt ccttcttttt
    14821 catttgaaac tgatcttgct ataagagtag cttgagttga gtggaatcca gaaacgatac
    14881 cacaagcaac tgtaacaaag aatgtaggaa taagaggaat accatcagga tgaatacctt
    14941 tccaatttgc aagacttaaa ttagctaaat cataaccttg tgtaaagatt ccaattccaa
    15001 caccagcagc agataaaagt agaatagctc caaatattgg atataccttt cctatgattt
    15061 tatcaattgg aaagagtgtt gcacacaagt aatatagaaa gataagtcca taaactatcc
    15121 agacaactgg attatttata gtcgatttca tatttaaaat ttgagtaaca actaaatccc
    15181 caggtgtata aataaaaact gcaccaacta aaagcattaa aagacaaagg aatatattat
    15241 agacttgaaa tactttatta cctaaaaatc tactgataag agaaggcatt tgtgctcctt
    15301 tttctcttat actaatcatt ccactcatat agtcatgcaa agctcctcca aatacacagc
    15361 caataggtat tagtatgaat gctattggac caaacaatat accttggata ggtcctagaa
    15421 ttggtcctgt accagctata ttaagcaact caattagact atttttccat tttttcattg
    15481 ggacatagtc tactccatca ctttgagcaa gtgcaggtgt ttttctgtca tctgggccaa
    15541 atactttttc acaataagca ccgtataaag cacctcctat tagtaatata gccaagccga
    15601 ttaagaatgt tgtcataata tcaccccatt tataaaattt tttggtcttt gtatacatat
    15661 tatctaaatg tgtagattta tattgaattt aattctggac aacctacaaa ttctataaag
    15721 tattacaact aatataattt cagaaataaa tacatataca taaccatgta cttagtatac
    15781 aggtatttgg taatattttc aatgttatag caataaagaa tataaacaaa attctgataa
    15841 tattttacaa aaaaattaaa aaatataaaa attagtttaa atagatggag aaaaaaggaa
    15901 aatcaattga gaatataaat gtctatatct taacaatatt aagaggttta aaattcttga
    15961 agaaagaata gtcaagtgat ataatgggtt tacaggtgta tatacactag tacatataga
    16021 aagcggtgca attatggata aagatttaac atatcaaatc aaagagctaa agaaagaaaa
    16081 aaatgcaata atacttgccc atttttatca accaccagaa atacaagaat tagcagatgc
    16141 agttggtgac tcatattatt taagtgagat tgctagagat tgtaaagaag aagtggttgt
    16201 attttgtgga gtaaggttta tgggagaaag tgctaagcta ttatcacctg aaaaaactgt
    16261 attgatgcca gtaagtaacg ctgggtgtgc tatggcagat atggtagatg aagaaggcgt
    16321 gataaaatta aaacaacagt atccaaatgc tttggttgta tgttatataa actctacagc
    16381 taaagttaag gcacattgtg atgtttctgt tacatcttct agtgcaatta aaatactaga
    16441 aaacatagat aataaagaaa ttatattcct tccagataaa aatttaggag gatatattgc
    16501 agaacaattt ccagataaaa actttatatt ttgggacgga tattgtaaat accataacaa
    16561 tataagagct gaagaaataa ttgaattaaa agataagtat aaaaatgcag aagtattagt
    16621 acatccagaa tgtaaaaaag aaataagaga tttaggagat tatgtaggta gtacatccgg
    16681 aataataaaa tatgctacta atagtaaaaa caaagacttt ataatagcaa ctgaagaagg
    16741 tatattacat gaattaaaga aaaataatcc taataaaaac ttttatattc ctggtggaaa
    16801 aatactatgt acagatatga aaaagacaac attagaaaat ttatatagta cacttaaaaa
    16861 tatggaaaat gaagttattg tagaagatga aatcatggaa aaggctttaa attcactgct
    16921 taacatgcac aaattagcag aaggatagag ggatagactt atgaatttag aacaagatgt
    16981 tttaatagtt ggaagtggag tttcaggatt atactgtgca ttaaatttag ataaaagttt
    17041 aaatgtgcta gtagtatcga aatcaacaat tgaaaacaac aatacatatc ttgcacaagg
    17101 tggaatttca actgctagaa atattgatga tatagaatcc tttgtagaag atacaatgaa
    17161 ggctgggcag tataaaaata gagttgaagc agttcaagta cttgcagatg aatctataga
    17221 aaatgtaggg cagatagtag aatatggtat gcctttagac aaagaaaatg gagagataga
    17281 ttacacaaga gaaggagcac atagtgtaaa tcgaatagtt catagtaagg ataatactgg
    17341 agaagttgtt tttaaaactt tattaaaaga agctaaaact agagaaaata taactcttat
    17401 agaagatgca tatcttttag atatattaaa agatggcaat aaatgtatag gagctagaat
    17461 ttttaaatct aaaaaagaga ttcatgtttt ttcaaaaata gtagttttag ctacaggtgg
    17521 aattggtggg ctttttaaaa attctactaa tcaaagacat cttacaggtg atggaattgc
    17581 aatagcttta agaaataata taaaaataga aaatttagaa tatattcaaa tacatcctac
    17641 agccttttat gaagaaaata atgaaggtag gagaatgctt atatctgaat cattaagagg
    17701 agagggagct aaacttttaa ataaaaataa agaaagattt gtggatgaac ttcttcctag
    17761 agatgtagtt tcaaaagcta tttttgagca aatggaaaaa gataaattac cttatgtgta
    17821 tttagatgca acacatttag atagtgaata tttaattaat agattttctt ttatttataa
    17881 tgaatgttta gctagaggaa cagatataac gaaagaatgt ataaaggtat ctcctgcaca
    17941 acattatttt atgggcggga tacatgtaga tttggattca aaaacatcta tggaaaattt
    18001 atatgcagtt ggtgaaatta gttgtacagg tgttcatgga gccaatagat tggcaagtaa
    18061 ttcattactt gaagggcttg tattttcaaa aagagcagct aaaaatataa actcagttat
    18121 agataatgta aaagttaagt ttatagatgt accagatatg gatatagata tagaacaggt
    18181 aaaaaaagaa aataaaatat tagtcataaa agaaattgaa agaacaagtg aggattttgg
    18241 cgatgaatta tttgattatt gatagaatga tacaggatgc tttgatagaa gatgttccaa
    18301 gtgaagatat aacaactaat tctattgtgg atgaaaattc taaatctact gtagatttaa
    18361 tttgcaaaca agatggtata atagctggct taggtgtatt taagagagtt tttgagatac
    18421 ttggtgatgt agatgtaaaa ttatataaaa atgatggtga tagagtaaag aatcgtgaaa
    18481 agatagctgt attaacaggt agtactagaa acttacttgt aggagagcgt gtagcattga
    18541 attatttaca gagaatgagt ggtatagcta ctattacaag taggtatgtt gaaaagcttg
    18601 aaggtacaaa tacaaaatta ttagatagta gaaaaacgat acctaatctt agaatattag
    18661 ataagtattc tgttaaagta ggtggaggat gcaatcatag atttaattta tctgatggaa
    18721 tattgcttaa agacaaccat attggggctg ctggtagtgt taagaaggca gttgaattag
    18781 ctagaaaaaa tacttctttt gttagaaaga tagaagtgga agtggaaact ttagacatgg
    18841 taaaagaagc aatagaagct aatgcagata taataatgtt ggataatatg agtttggaaa
    18901 tggcaaaaca agcagtagat attataaatg gaagagccat tgtagaattt tccggaaatg
    18961 taaatttaga tactatagaa gatataggaa aaattggtgt ggatgttgtt tctgtaggag
    19021 cgcttacaca ttctgttaaa gctctcgata taagtatgaa gaatttgaga aatatataag
    19081 ttttatattg aaataaactt aattaaaata gactccttaa atttgtttta taaatttaag
    19141 gagtctattt tttattcatt aaaattaata aaaatataaa aaaatcaatc aataatttaa
    19201 taaaaataga taaatactat atacactttt aataatatag ctaatatcta ttttacagat
    19261 aaataagaat aaacttataa tataattgta tctgtaaaat tttgtaaaat ataaaaagta
    19321 tagggggaat aaaaatgaaa gtagcttata tttttagtag tactaataca cataaaattc
    19381 tagacaaaat gataatacca caacttgaac aaggaactca tggagtagac gttctaggga
    19441 tgtttttctt tatggataat accttatttt tatctaaagg taatccagta ggcgagagac
    19501 taagtaaatt acatgaaaaa actgatatga taataatggc ttgtgaccaa tgtgctatag
    19561 aaagaggtat agagaataat ttggttgatg gagctacaat tggatgtttc ccaaacctat
    19621 atgcttgttt aggcagtgca ggtgtggacc aagttataac gttataaatt agtataggat
    19681 acaatccata gaaaaattta ttttctgtgg attattttaa gtaaaagata aattgtttaa
    19741 agtaggggat gaacaaatgt ctaaaatttt agttgaattt ataaacactt gaccatcttg
    19801 tgatgaacat ggagtgttta taaaaaaaat gtcagaaaag tatgctgata aattggaaat
    19861 aaaattatat caagcaggga aagatttttc gtatataaaa aaatatggga ttataacaaa
    19921 aggaacttta ataataaatc agaaaaagaa atatgataga ttaaataaag atacaattga
    19981 aagggcaata gtagaagcta taaacaataa ttaaaagttt aaaaattagg agtatcaaaa
    20041 atgaatatat tagaaattgt tttaaatgga gttaaagata ctatagtagc ttctatagtt
    20101 acactgaatg gagcatcagg atggcttatg ataagtttta tattggcagg tgtacttcac
    20161 aatgttttaa ctccaagaag gtttcaaaaa caacttggaa ataagaaatt ttcttcttta
    20221 attaaaagta ctatctcagg aatgttttta ccaatatgta gttgtggagt aataccactt
    20281 ggaataagta tgtattattc aggagcatat ttaggaccag tacttgcttt tatgacatca
    20341 actccaataa taaatccaat agctgtagtt ctttgtctag gtcttttagg tcctaaagtt
    20401 acaatcattt atctaatagc tggtttttta gtaccatttt tagtaggatt tttaggaaat
    20461 tgttttggaa aagaagaatt atcactttat aatgattcag aggaagaagt aatagaatta
    20521 gaagaagaaa aaagaagttt tatggaaaat atgattgatg gtcttaaatg gtcttttggt
    20581 gaattagccc ttactattag taaatatgta gtaatgggta tggtttttgc aggatttata
    20641 acaactattt ttccaaatag tataattcaa aaatatttag gaaatccagg tttgctatct
    20701 cttggaagta tagcaatact tgcatgtgtt atgtatgtat gtgctgtagg acatataccg
    20761 tttatagcag cccttgttgc aagtggagca tctcctggta tagcgataac atttctaata
    20821 gctggagcag ctacaaatct accagaactt ataagcatgt ttaaacttat tggaaaaaga
    20881 actgttatta tatattctgt aactttgaca ctgtcctcaa taataattgg atatataaca
    20941 aatttacttt tagtgaataa taattcagta aatttagatg caacaaattc ttcaatacag
    21001 ttggcaaata aactaatgtt aaatatacct gatagttttg agtatgtatg tactatttta
    21061 attattttat ttgcatttaa atcaacaata ccaaagatga aagaggtcat aggaaatcat
    21121 gcttaaaaag atagttataa tatcctgtat agtagttgtg ttaatcattt tatcaaaaat
    21181 agttgatgat aacatcaaag aagatgcttc tattccaaat gtgaataaag aaacattaga
    21241 atattttaga aaaaattata aagaagacat aataacttgt gctgaagaag atttaaataa
    21301 tgatgggaaa aaagatttag tagttatata taaaaaaagc aataattcaa atgaaatggt
    21361 agtcgtagtt tctgataaaa atagccacta tataacaaag ccaataccag cacctataga
    21421 gaatcaaact ataaccttta aaaatattga tgataaagca ccaatagaag taatagtatc
    21481 agggtctaaa aatggtaatg taggatatgc tatttacaga gtagagggta aaaaatttgt
    21541 tgatttattt ggcgaagata tggacaaatg ttgctaaaaa taaaaataat acataaaggt
    21601 gggaaattta tatgaaatta tctaagaaga ttcaagcact aatcttattt ggtgtaatgg
    21661 gtacatctat attaacagga tgtagtagta aaccaaaaga aaaagaagaa gcaaaagctg
    21721 gaaaagatga ttatacaata acagtaggtt attataattg tgaccatatg actgcgggac
    21781 cagtagcaga atcggcagga atatataaag atttaggtct taatgtaaaa actgttggaa
    21841 atggtaaagt tcctgaagct atggcagcag gaaaaatgga tgcaggttac ataggaacta
    21901 aggggttagt tggagcaata cctaagggtt caccaataac aatagcagct aacaaccata
    21961 ctggtggttc tgaatattta atagtttcaa aagatataaa agaacctaaa gatttaatag
    22021 gtaaaaaaat tgcaacagat atgagtgatt tcttatggac aagtgattat ggtccagaaa
    22081 caggtctacc aactgaccca tcaaaatatg aagttgtaaa tatggattca gacaaagata
    22141 aatatttagc tcttaaaact gggaaaattc aagcttttac atcttgtgac ccttggggtt
    22201 ctgtagctga aaatgacggt gcaggaaaaa taatagcatc tactcaatat aaagaaaaag
    22261 ctaatggcaa agaatataac tgttgttcat tctcattaaa caaaaacttt ataaaagaac
    22321 atccagattt agcaaaaaaa ttggttctag cacatacaaa agcaatagaa tatatatata
    22381 caaatccagc tgaagcagct aaaatatttg caaaatatta taatgtagaa gaagaagttg
    22441 cacttagaac tatctataag aagactgttg gagaaggtag aactcttact tggaaagtta
    22501 caggtgaaga atataaaaat aatttacaaa tgtataaaga tttaaaggct ttagatgata
    22561 tacctaaata tgaagatacg attgatacaa gcttattgga ctcttgtgga gctgatgatt
    22621 ttgacaaatt tataaaagaa aaagttaacc cagagtttcc tgaaggtatg agttatgaag
    22681 actggaaagc aaaggtatta taaataaatg gagaatataa aaaatataat aatacttttt
    22741 atcattattt tgggaagtat atttataata ttttttaatt ttgaagataa agaagaaata
    22801 aaaggaaaat taagaatagg ggtatctgat gataactctg gttttgtaat aaattatatg
    22861 gtaaatagag actactttga aaatataaaa gtaagtgata taatggatat ttttacaatt
    22921 aatgattgct gagcaagtac aatgcagtgg gctctgagtt cagaggaaat agatatagca
    22981 atcatatgta aagatgctgc aaaacaatat gtaaatgtaa atagtggatt tgaaattgtt
    23041 ggtactgtag ttcaaaattc tgatatattt ctattaagtg ataataatcc tgaaaagata
    23101 ggagtcattc aaaatagaga ttaccagtca gaattggtta aaaaatacta caagaatgtg
    23161 gaagtagctc cattattagg gactgcacta gcatatggat tagaaagtaa tttagtagat
    23221 ggtgttgtaa tagattgcat aaagtcaatt gggttagagg gtaaaagaaa atctacaact
    23281 aaattaggag attatgatac ctatgtatta gttgtaagta aagagtttaa aaaaagtaaa
    23341 gcatatagtg attttataac actttacaat aaaagtgttg atgctttaaa gaataaagct
    23401 acatttaaga aagcattaaa agagtacagg aatataagaa atatagatat ggaggaaata
    23461 agcaattgga aattaaaatt tctaaagata aaagcgatag attaactaag aaaaaaactg
    23521 atggtagtat ggatatttta tataaaataa tactattagt tattatattt ttagtctggc
    23581 attttgcagc aaaagatata gggagttctt tgttacttcc aatgccagta gatgtaataa
    23641 aaggtttctt tttctgtgtg acagatgcag agacagtcac taatcttttt ataacattgc
    23701 agagagtact taaaggtttt atgtatgcat tattgtttgg gcttccaata ggatttatta
    23761 tgggattttc taaaactttt gaaagggttt tatctccagt agtagattct gtaaggcaag
    23821 ttccaataat ggcatgggtt cctcttacta tagtatggtt tggtatagga gatggtccaa
    23881 caatattttt aatagctttt tcaggggttt tccctataat tttaaatact attcaaggag
    23941 taagagcaat atcaaaggat tattataatg cagctagaag tatgggagca tcgccaattg
    24001 tgatatttac aaatgtaatt gttcccgcat ctcttccaga tatactaact ggttcaagga
    24061 ttgctataag tacaggatgg atgtctgtta tctgagccga gtttatagct acgagtgccg
    24121 gtctcggtta ctcaatggta caagctcaaa cattaatgca tacagatgta ttaattgggc
    24181 ttatgatatt tgcagcttta ataggattta taatagatag ggtattaaag tttataaata
    24241 aaatcctttg tagatggagg tttgcagatt aatgaagtta agtgtaagag atataaataa
    24301 gacatttgta aataatagag tacatacaaa ggttttagaa gatataagta tggatataga
    24361 tgatggacaa tttgtctgtc ttttaggacc atcaggttgt ggaaaaacaa ctttacttac
    24421 tataataggt gggtttcaaa aatcagaacg tggagatgtt tttataaatg ataaaagagt
    24481 taaaaaaccg ggcatagata gagcttttat atttcaaaat tatgctttat ttccttggaa
    24541 aactataaga ggtaatgttc tatttcctat gaaacagcag aaaataccaa aagaaaaaag
    24601 agaagaaatg ctagaagaac ttttggttat gtcagacttg aaaggaaaag aaaaactgtt
    24661 tccgcatcaa ttatctggag gaatgaaaca aagagtggcc atgataagag ctttagcatg
    24721 taatccagag gtattactaa tggatgaacc attaggagct gtagattttc aaatgagaca
    24781 aaatttacag gaagagctag aacgaatatg gataaagaag aaaataactg cattaatggt
    24841 aactcatgat gttgatgaag ctgtatatat gagtgataga gttatagtaa tgtcgagaga
    24901 taaaggaaga attattgatg atataaatat agatattcca agacctagaa ttagaggaag
    24961 ccaaaaatat gaagagtaca aaaacaaatt aactgatact ttatcaaagt gttatgaagt
    25021 ttaaagagga gtagacatga gtcaaataga aaaatacaat agtttggata atacacaaca
    25081 gaaaataaga atctctatta tggatatgat aatagataaa ggttctagtg ttacacttca
    25141 agaagtgact gaatatgtaa gtaaaaagtt gaatatagaa aaagaatata tagaaaggac
    25201 actacaatat tttatttatg aaaatataat ggttgttgat gggaatagta taaattttat
    25261 atatcctgta tcagcattgc aaacaaatca taaagtaact ttgagagata atagaagttt
    25321 cagcgcaatg tgtgctatag atgctatagg aacctcttgc acattcaacc aagatataaa
    25381 aataaactct atttgtagtg ctacaggtaa ggaaatagaa gtaattataa aaaatgaaaa
    25441 gattgagtat gtgaataatc caaacttaag agtgcttcat ataaatcttg ataagcatct
    25501 aaattgggct gctagttgct gaaatataat gaatttcttc tggacgaaga gtgattttga
    25561 tgcttggaca aacgaagcgg gattatctga tgatgaagat atatattgtt tagatattaa
    25621 tgaggcaata gtagaatcat ataaaatatt taaactcaaa caaaaggtac tatcatagat
    25681 atacatatat gaaaaactaa aaatgacctt tagataaaaa tgataggtaa tatccatact
    25741 atataaatta gtatagagta gggatgtgaa aagtatttaa atctaaaggt atatttttag
    25801 tttttttaga tgattagata gccttattaa gtatatattt atttataacc tttgcaactc
    25861 cattttcatt attagtatca gtaacataat ctgcaatttc ctttatacta ttacgtgcat
    25921 tccccatagc cacaccaaga cctgcatact ttatcatatg ttggtcattt tcttcatcgc
    25981 caacagcaat agcattttct ataggtatat ttagattcgt acataatgtt ttaagagcag
    26041 ttcctttatt agtagtttta cctaaaaatt ctagataaaa aggagcagac cttactatag
    26101 tatatttatt gaataaattt ttaggtagtt gctgtatgat ttcttccaat ctttctggtt
    26161 catcaactat cataatttta cagattttaa tagattcatc aattttatct ttttgtatat
    26221 atttgacttc tatattattt aatttcgctt catgaagtgt atattttcca ggtgcttcat
    26281 ttggtgtaat taaatttgtc attgtattta tgtgtatttt agtcttatat tttttacaaa
    26341 cgttattata tataaaatca aaatcatcta aagtcatttc tacatcatgt atgattttct
    26401 cacttttaac ttcttgaata attgctccat taaagcatat aacataatca ccttcatctg
    26461 ttaaatttag ttgatttaga agttctttta caccaggaag aggtctgcct gttgttataa
    26521 ctacttttgc cccagccctt tttgcctcct gaatggactc aaagacttca ggagtaatcc
    26581 ttttttgtgt atttaatatt gttccatcaa tgtctagtgc gattaatttg tacatatcaa
    26641 cctccaataa attatatact caaatttcct gtgaaaacgt tgacacagct agtgttgaac
    26701 aaagtttcag tgggaaatat ggtttgtttt gtatactatt atatcaagag ataagtaatt
    26761 tagaaagttt aataatatta atttttgata aaaatattac ttatgtgaga tgagaatgta
    26821 aaaactttgt aatcaataat tccaaaattc ttaattacaa ataatggtat cgtttgcatt
    26881 gttaatataa ataaatgtat attatatagt tagaagtttg aataaataga ctgatggtaa
    26941 tttgttatat tagttagttt tgtgttataa aatattgtaa ttcc
//
LOCUS       DAMJWP010000028        26620 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.029, whole genome shotgun sequence.
ACCESSION   DAMJWP010000028 DAMJWP010000000
VERSION     DAMJWP010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26620
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.029"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(118..294)
                     /locus_tag="PC700_001063"
     CDS             complement(118..294)
                     /locus_tag="PC700_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580383.1"
                     /translation="MFRDKMDRCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(348..444)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1240..1641
                     /locus_tag="PC700_001064"
     CDS             1240..1641
                     /locus_tag="PC700_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HDF2580384.1"
                     /translation="MNYKNRKRAYYQENSCMDNFICKNCGCLVVPEGAGTQHRNHCPH
                     CLHSLHVDIIPGDRAADCDANMEPIGVWVRKNGEWAIIHRCTRCGHLSSNRIAADDNS
                     VKLMSIALKPLAQPPFPLERIIEKLEKEETE"
     gene            1638..2705
                     /gene="rsgA"
                     /locus_tag="PC700_001065"
     CDS             1638..2705
                     /gene="rsgA"
                     /locus_tag="PC700_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDF2580385.1"
                     /translation="MNGMKQINLQDYGLNRNFITQSMEYHDLYLARIISQEKNFYQAV
                     CVNGVLFAEVSGKLRFEATIQSDFPTVGDFVMLDRCNNKNGNAIIHHVLHRKSLFVRK
                     AAGKVNEEQVVAANIDIVFLCMSLNKDFNIHRLERYLSLTWESGAIPVVVLTKSDLCD
                     NVHRKLQVVNSIALGVDVVVTSSIEPDGYKQISKYIKSGCTVALLGSSGVGKSSLINC
                     LLGGEFLNTKEIRIDDDKGRHATTRRELFLISGGGIVIDTPGMRELGMWDVSEGLDKS
                     FADIEAYFGKCHFRNCTHTSEPGCAIWEAIAHQELSPKRWESYRRLKTEAVFADNKSE
                     YLAKKEKKFKNISKINKTIRR"
     gene            3241..3939
                     /locus_tag="PC700_001066"
     CDS             3241..3939
                     /locus_tag="PC700_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580386.1"
                     /translation="MKISSQYRSQYSFRHESNINNTRINESMVKKNETVGKDTYLSNI
                     MKQKQELNDRIRDLKYRQEVYTKKINEAIKNLYKSEIRETTNNFSNIEIGIKNSIIED
                     KNKSTMLDENSTYLNTNDEKESLITKESNEKIEEEILNDEKLEELEQKKDYKEDSNKK
                     EKVSEDLSLVGKTREELENMLKNFINLTQEEIMKLESRIEKLDKDAEEYKQNSKTNIF
                     DKTDEQKKHINVLI"
     gene            4041..4589
                     /locus_tag="PC700_001067"
     CDS             4041..4589
                     /locus_tag="PC700_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580387.1"
                     /translation="MEGLGFYAAALSGSSYQRIGFGKLDPIEVIADGDWISYKQAQDT
                     LTVIRNFLNSFDWRNASEMERANRAAKLVTEAKYVDSKYCNIVYGNLVDKRGVCGSFA
                     SSFHLLTRLMGMDSLSILNPSLNHAWNYIQIDGKWYRSDGSEISAFGGALDFDYRKLK
                     DATREMTTYYDAKALSILGFNQ"
     gene            complement(4990..5946)
                     /locus_tag="PC700_001068"
     CDS             complement(4990..5946)
                     /locus_tag="PC700_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2580388.1"
                     /translation="MENNTIQKQLRDLSKKIWHIYILNPEEENLKFIISLLDNNLALI
                     GTGKHEFYETLEEFIIGLEKDQTESEQIDFEIIDEWYSCTKVTDEAFIVYGTLWVIEK
                     DYLEKDIYVEMDTRFTMVYRVTDDMIKLIHVHHSIPNFDQMKDEYYPKTITEKAQEAL
                     AMANEFKLKSEKDLMTGLYNRHFFEVHIKEMLKTVSYGYFYIFDLDNFKQVNDIFGHV
                     RGDEILIQFSNILTKVFVNDAIIGRLGGDEFAVFEYSNQSKENVENKAKKVLELCLES
                     NSCSIGISKLENKSDTFQSLYKQSDYALYYSKRNNKGNFSWF"
     gene            6649..7644
                     /locus_tag="PC700_001069"
     CDS             6649..7644
                     /locus_tag="PC700_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2580389.1"
                     /translation="MSIIELNELTKSYRRFEKKAGFMGSVKSLFNRKYTEKTAISSFD
                     LKIEEGEFVGLIGQNGAGKTTLIKMLTGIIAPSSGEISVMGYYPNKLENNFKKQYAVV
                     MGQKSQLFFELTPADTFLLFKELYDISNEEYKKNLDYFIELFEVSDYLNVQVRTLSLG
                     ERMKMELIVALLHNPKILFLDEPTIGLDAIAQKQIRYFLKEVNETKGTTIILTSHYME
                     DIKSLCSRSIVVENSRKIYDGSTEKLFNHFQKYKKITVSFSNETNIVFPKDCKVVEQT
                     NHKTSVIVLKERGNETIKWIFDKYDVKDVMIEEEDIGSIVERIYKKGSDEYYEQL"
     gene            7622..8485
                     /locus_tag="PC700_001070"
     CDS             7622..8485
                     /locus_tag="PC700_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HDF2580390.1"
                     /translation="MSIMSNYNEASSNYSMFYKFKQSIRRYYEVAKINFKVQLIWRFD
                     VVVNMVFTIVKILFAYILWKAIFGENKIVSGFTFNSMLSYYIISSFLAGIDMSKSTSE
                     EICNRVKLGTFSKYMIIPINVQNYFIAKNFGMTCFYFVFNMISAFVWIFIFKIDFAFT
                     SNPVQILISILMIFLGFIFMMKLHFFIGILSFKFLEISFFRMIIENIVEFITGTMIPL
                     VLLPETIVTVMKVFPFYYTTYLPSMLLIGRNSNEAIQGLIILALWVLFFSVVNKVMYK
                     RMRTLFDGVGI"
     gene            8482..9276
                     /locus_tag="PC700_001071"
     CDS             8482..9276
                     /locus_tag="PC700_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HDF2580391.1"
                     /translation="MMKKNLKIIFMLLKMKLSKMMVFRFDFFGAFFVDSSLFILQLLM
                     FNSIYSHVDSIGGWQQGEMLIFIGTFSLINAINMTIFFFGIYDIPRKIQEGELDYYIT
                     KPLDPLFRLTFENINIGSSPLILASILIVLYGISKLNSEITLVMIIIYIFMVLLMTLL
                     FYDVCIILRTVSFFVISSSAIMRLEDNLLPMNMKIPGVIYEGVFKVLFYLILPYGIMS
                     TIPTQILAGTITINGLIYSILIVFLFTLFMWRFWKLGMRHYKSASS"
     gene            9561..10940
                     /locus_tag="PC700_001072"
     CDS             9561..10940
                     /locus_tag="PC700_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2580392.1"
                     /translation="MSDVYYSENHEQDNLYEQDNLLAQRFNLFTLLKFAFPTIFMMIF
                     MGLYTIVDTIFVSRFINTNALSAINIVCPIINLIVGIGTMLATGGSAIVARKMGNGDI
                     EGANKAFSLIVLSGVVIGVIITGIGIVFMDYIIIWLGASEVLIKYCRSYLFIILVFAS
                     ANILQVLFQCLFVTAGKPQFGLWLVLGAGCANIILDYVFIVLLQMGISGAALATGVGY
                     LIPTVSGMVFFSKNKELLKFCIPRLDKRILFEACFNGSSEMVGQLSTAVTTFLFNAMM
                     MKLIGEDGVAAITIIIYSQFLLTSLYIGFSMGVAPIISYNYGSENNTQLNHVFKICIL
                     FISVASLLVFIFSISCSPIIVEIFSQRGSRVYEIARSGFYIFSFSFIFCGYNIFTSSM
                     FTALSNGKVSAILSFLRTFGFITIGLLILPNILNINGIWLAVPSAEILTLFLTIFCIC
                     KYKNRYKYM"
     gene            11395..12108
                     /locus_tag="PC700_001073"
     CDS             11395..12108
                     /locus_tag="PC700_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2580393.1"
                     /translation="MDTYFNKKILLVDDEKDIVDLIEEVLINDGFKNIIKAYNGLDAI
                     SLCKIACPDVVILDIMLPDIDGIEVCKKIREFSYCSILFLSSKNDDIDKILGLSSGGD
                     DYITKPFSPREIAFRVKAQLRRQQYQSIVPSDSAVIKIGDITIDIEGNRVYKDRNEIE
                     LTGREYHLLSYMAQNVNKIIGKERLYEQVWGVYSSICDNTIMVHIRHIREKIEDNPSN
                     PKILITVKGLGYKLVNRID"
     gene            complement(12380..13918)
                     /locus_tag="PC700_001074"
     CDS             complement(12380..13918)
                     /locus_tag="PC700_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF2580394.1"
                     /translation="MSLLKVTNLSQCFMDKSLYEKANFDLFKGEHIGVVGQNGTGKST
                     LIKILLGEVVPDSGEIKWQPNINIGHLDQYAEINRDTTISLYLHTAFEELYKIEKEMN
                     LLYQKSAISENEQYLIKASDYQEQLIASNFYSIDNEINKIANGLGLDSIGMNRVVEEL
                     SGGQRAKVILAKLLLSNHDVLLLDEPTNFLDKEHVEWLSNYLNTFNGAFIVVSHDFDF
                     LEKISTGILDIEFGMIKKYHGKYSEFLKQKSHLREDYIRRYQAQQKKIEKEETFIRKN
                     KAGVNSKIARGRQKQLDKIERIAPPSFTGKPNIQFSEIEISAQNALTITNLEVGYYYS
                     LLPKLNFSVDGGQKIVITGFNGIGKSTLLKTLVKDIPRISGDFQFSEQVKIGYYEQDL
                     KWENPDKTPLQIVADKYPKLNTKEIRRHLARCGVKEEHVSRSVSTLSGGEQSKVKLCC
                     MMLSPCNFLILDEPTNHFDAETKDALQNALKQFRGSIILVSHEEKFYKGWIDKVFNIE
                     KQLV"
     gene            14801..15256
                     /locus_tag="PC700_001075"
     CDS             14801..15256
                     /locus_tag="PC700_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HDF2580395.1"
                     /translation="MDEIDLKIINSLKKNSRASTSEISRQINLSVPAVSERIRKIEQS
                     NLIQKYTIQINREKTKYKLLAFIFINTDLSKSMKEFKKTILEYDTVLECHHISGEYDY
                     LIKVLAEDTSNLEEFISVTLKEIGGIQKANTTIVLSTIKEEFNTIKDIF"
     gene            15305..15889
                     /locus_tag="PC700_001076"
     CDS             15305..15889
                     /locus_tag="PC700_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HDF2580396.1"
                     /translation="MKVLVFLAKGFETMEFSVFVDVMGWARNDYGHDIDVVTCGFKKQ
                     VMSTFNIQVLVDKTIEEVCVDDYDALAIPGGFEEFGFYDEAYDSSFLNLIREFNSKEK
                     IIASICVAALPVGKSGVLKNRKATTYHLKNGKRQRQLSEFDVNVVNEPIVVDKNIITS
                     YCPETAPHVAFKLLEMLTSREQMDEVKLAMGFKL"
     gene            complement(15894..16217)
                     /locus_tag="PC700_001077"
     CDS             complement(15894..16217)
                     /locus_tag="PC700_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2580397.1"
                     /translation="MKKCLDNYSCPIEATLALIGGKYKTLILWHLKDTILRFNELKKL
                     IPKATPKMLTQQLRELESDGLIIRVVYPVVPPKVEYSLSDFGKSIIPILDSMCDWGSD
                     YLESL"
     gene            16369..16887
                     /locus_tag="PC700_001078"
     CDS             16369..16887
                     /locus_tag="PC700_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2580398.1"
                     /translation="MNFVELTKKRYSCRNYQDRKVEKEKLEKVLDVARIAPTGGNRQP
                     QRLIVIQEKEGINKLSKAANIYDAPLAILVCGDKDKVWTRPFDGRQLTDIDTSIVTDH
                     MMLQATELGLASVWVCYFNPDIIREEFSLPDNLEPINILLMGYESKIPESPERHEKTR
                     VPLSEIVSYETL"
     gene            complement(17063..17731)
                     /locus_tag="PC700_001079"
     CDS             complement(17063..17731)
                     /locus_tag="PC700_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580399.1"
                     /translation="MLEISHKVKEIYPNIKFGVMVINITHSKPNKENFLVLKNSTIKN
                     IIEQHPEYNRKEKIKTEPVSSYIKYYKKFKKTYPVLLQLESILLKSKGIPDVGVTIES
                     MFLAELKNLLLTAGHDLDKMELPLKIELANGSEHFYGIGGKEHILTKDDLFLSDNIGI
                     LSSILNGPDNRTAITKDTKNIMYFVYGPDKISEKQIQDHLNDIKHYISSAFPDLKVNS
                     IDIF"
     gene            17846..19261
                     /locus_tag="PC700_001080"
     CDS             17846..19261
                     /locus_tag="PC700_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2580400.1"
                     /translation="MWITINRNSNISLSRQIYCEIKDMILDGSLKFEQKLPSSRTLSK
                     ELSVSRNTVLDAYNQLIAEGYLETRNGYGTIVAKGISGHKVDFKLNDTFRDEKDLHTN
                     KQKNYIDFRSGVPELKLFPRKELSKLYKQILYDLSDSDFRYGSTAGVWVLRVEISKYL
                     FRTRGIRCNSKNIMIVSGSTQGLSLISQLLYKSNKKVAVEDPIHKGLLKVISSIGYSV
                     VGIKVDDKGMNTNLIKSIKGISFVYTTPSHQYPLGGILPIQRRLELIKCATENDYYII
                     EDDYDSEFRYEGQPISSLYELSPNKVIYIGSFSKILSPAIRVGFMLLPDNILESYKTL
                     KMYSDVHTETILQYVLAEFIGNGGLEKYIFRMKKLYNRKRKYLIKVLNTEFKNEFKIK
                     GEAAGLHIVAHFYNVKFTESLIHKIFEAGIKVYPIQQYAIENILEYDHEIILGYAHLS
                     FDEIEKGVKILSKIIHMNQGT"
     gene            19484..19942
                     /locus_tag="PC700_001081"
     CDS             19484..19942
                     /locus_tag="PC700_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2580401.1"
                     /translation="MEIIDYIEENLTENLNLDKIAYIANYSKYHLHRMFTNVVGCTIH
                     QYIQRRRLTEAARQLIFTDKYIIDIAITVGYSTHQSFTLAFRNLYKESPQVYRNKDEF
                     YPIQQRFVLNLNHQIQSEKCISYANMNYKQRRDEINSYLNFNINCEVKVA"
     gene            19939..20523
                     /gene="clpP"
                     /locus_tag="PC700_001082"
     CDS             19939..20523
                     /gene="clpP"
                     /locus_tag="PC700_001082"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDF2580402.1"
                     /translation="MSLVPHVIEQTGQGERSYDIYSKLLKDRIIFIGEEINDAIASLV
                     VAQLLFLESEDPDSDIIIYINSPGGSATSGFAIYDTMNYVRCDVSTICIGMAASMSAF
                     LLAGGTHGKRCALPNSEIMIHQPMGGAKGQATDVKIAVDNILKIKERLDKILSENTGK
                     SIEDIRRDTDRDNFMTALEAKEYGLIDYIMNRNE"
     gene            20845..21921
                     /locus_tag="PC700_001083"
     CDS             20845..21921
                     /locus_tag="PC700_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2580403.1"
                     /translation="MIAISLCMIVKNEEEVIGRCLECVKDIVDEIIIVDTGSTDSTKE
                     IVSKYTDMVYDFEWIDDFSAARNFSFSKASKDYTMWLDADDIILEADREKLLKIKESL
                     DTSIDIVMAKYNVSFDENGNPTLSYYRERLFKRSSNHKWVDPIHEVVPLFGEVFYSDI
                     AISHKKLHRQDPLRNLRIFEKMISEGKTLEPRHQFYYSRELYYNARYKEAIEGFTKFL
                     DSSRGWIEDCISACRDLATCYYLINDEKSALYSLFRSFEFDEPRAEICCDIGKHMFDR
                     QKYKEAIFWYKVALTRDKNDTNGGFKSNDCYGYIPSIQLSVCYDRLGESDKAIYYHEK
                     TKEIKPNDSAVLHNENYFSKFKNS"
     gene            21988..24027
                     /gene="bclA3"
                     /locus_tag="PC700_001084"
     CDS             21988..24027
                     /gene="bclA3"
                     /locus_tag="PC700_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA3"
                     /protein_id="HDF2580404.1"
                     /translation="MLLIMSRNKYFGPFDDNDYNNGYDKYDDCNNGRDDYNSCDCHHC
                     CPPSCVGPTGPMGPRGRTGPTGPTGPTGPGVGGTGPTGPTGPTGPTGNTGNTGATGLR
                     GPTGATGGTGPTGATGAIGFGVTGPTGPTGATGATGADGVTGPTGPTGATGADGITGP
                     TGATGATGFGVTGPTGPTGATGVGVTGATGLIGPTGATGTPGATGPTGAIGATGIGIT
                     GPTGATGATGADGATGVTGPTGPTGATGADGVTGPTGATGATGIGITGPTGATGATGI
                     GITGATGLIGPTGATGATGATGPTGVTGATGAAGLIGPTGATGVTGADGATGATGATG
                     ATGPTGADGLVGPTGATGATGADGLVGPTGPTGATGVGITGATGATGATGPTGADGLV
                     GPTGATGATGADGVAGPTGATGATGNTGADGATGPTGATGPTGADGLVGPTGATGATG
                     LAGATGATGPIGATGPTGADGATGATGATGPTGADGLVGPTGATGATGATGPTGPTGA
                     SAIIPFASGIPLSLTTIAGGLVGTPGFVGFGSSAPGLSIVGGVIDLTNAAGTLTNFAF
                     SMPRDGTITSISAYFSTTAALSLVGSTITITATLYQSTAPNNSFTAVPGATVTLAPPL
                     TGILSVGSISSGIVTGLNIAATAETRFLLVFTATASGLSLVNTVAGYASAGIAIN"
     gene            24196..24957
                     /locus_tag="PC700_001085"
     CDS             24196..24957
                     /locus_tag="PC700_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HDF2580405.1"
                     /translation="MKNKIETSMNNFVKNYHKSNNITTKWQELLFAYASAEDDMFQKL
                     KSVVGTSHAMPKDFLENGKTIITYFIPFDESVNNSNIGGFECSEIWARAYIETNMLIS
                     NLNDFLCEELEKMGANSYAIPGSYNFDSEKLVSDWSQRHVAFIAGLGTFGLNNMLITE
                     KGCCGRIGSIITSLELEPTKRPENEYCLYKHNNSCKKCVNKCVKEALKEDGFNRNKCY
                     EMCMINEKKYTYIGGCDICGKCIVGVPCSVINPSR"
     gene            25266..25544
                     /locus_tag="PC700_001086"
     CDS             25266..25544
                     /locus_tag="PC700_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580406.1"
                     /translation="MSYKKAIHILPEELLELIQEYVDGEYIYIPRKSNNKKEWGSSTS
                     TREDLSIRNMQIYEDYQVGYDLKYLSEKYYLSLKSIQRIVLQEKRKDS"
     gene            25837..26412
                     /locus_tag="PC700_001087"
     CDS             25837..26412
                     /locus_tag="PC700_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1062 domain-containing protein"
                     /protein_id="HDF2580407.1"
                     /translation="MKKITWEVEYISQLPVIRYCKKCGKKTEYICSGLFRVNAQHKYL
                     DIWLIYKCSNCDSTWNSTIYSRVNPKSLNPETLEQFHTNDYNLAKQYAMNTELLHRNG
                     AEVGIPEYKIVGQEININDLTQVHIISKYPCQLKVSTLLREKLGLSRKVFDEMLDCGI
                     IRSNSGVDLKKGKLNYEIMLDIGKEKQKSSN"
BASE COUNT         9882 a         3204 c         4469 g         9065 t
ORIGIN      
        1 tattttaaat agaaaataaa attatgaata ctagaaatct atcttaatta gctttctaac
       61 ataaggctat ttaaagtaga taaaactaaa aagggttctc tagaaaacca aatctattta
      121 attatactta cttactaata ccatcacttg atgcaaacaa gattctacca cattttctcc
      181 gtactctagt ataaaatcat ctagttgtgt atctataaaa tcacaataat catatgaact
      241 accaatataa gcagtcaaca tatgtgtaca tctatccatt ttatctctaa acatatatat
      301 tattatcccc ctaaaaaact ttcataaaaa caaaaagaat atctcttttt ggcaactggc
      361 tgacataact catagtttat tttataagcg ttagtcccta tagctttgcg tcactaaatt
      421 tctctagttt tgccgattta gttttattct acaattacaa taatacaata gaattgctta
      481 ttattcaata tacttatttg aaaattaaat aaatttacaa atatgaacgt tattttatat
      541 aaaaaaaata gactattcat gtagaatata tggaaaaact tgattgtagg ttttgtatga
      601 aaagacagtt gttattaatt taagtgagat aaattgagca ataggcataa aatgagaaaa
      661 caaataatag caattaattt ttattattaa tactaggtga acattaaatt ttaaaatacc
      721 actagatttt atagaataag aaaatgagac aaacataaat atatgaatat attaaattat
      781 acttgaacat acttatattt tatgataaaa ttttaataat aattattagt gaaggagtgt
      841 cttgcctgat gtcagagtta tatataatgg taaccaacaa ataaatagtt ggagacgcat
      901 atgggttctg tttaatcaga atttttaagt gtatttttga tacatttgat tgttgtgtct
      961 tactctgcct ttatagataa ctaacattag acaacaaata ataatattac atattacttt
     1021 gttaaattgt ggtgtagtgg gctttaatct gctacacttt tattaatttt acacagtatt
     1081 attttgaata taattatgtt gaaatagaag atactaaatt ttctagtcca caggggtttt
     1141 ctgtttttgt gtaggtaaat atgtatacca cataaaaagg cagatataaa tacctgtgga
     1201 ctttttttgt agaaaataca agatacggag gaaataaaaa tgaattataa aaatagaaaa
     1261 cgagcttatt atcaggaaaa ttcctgtatg gataacttta tttgcaaaaa ttgtggttgc
     1321 cttgtagttc ctgaaggagc tggtacacaa catagaaatc attgtccaca ttgtttacat
     1381 agtcttcatg tagatatcat tcctggtgac cgtgctgcag attgtgatgc taatatggaa
     1441 cctattggag tgtgggtgag aaaaaatggt gagtgggcaa ttattcatcg ttgtacacgc
     1501 tgtggacatc ttagttctaa tcgtattgca gctgatgata attcagtgaa attaatgtca
     1561 attgcattaa aaccactggc acagccccct ttcccactag aaagaatcat tgaaaagttg
     1621 gaaaaggagg aaacagaatg aatggtatga aacaaatcaa tttacaagac tatggtctaa
     1681 ataggaattt tattactcaa agtatggaat atcacgattt ataccttgct cgaattatct
     1741 cgcaggaaaa gaacttttat caagctgtat gtgtaaatgg tgtactgttt gcagaggttt
     1801 caggaaaact gcgttttgag gctactatac aatctgattt tcccacagtt ggtgatttcg
     1861 ttatgttgga tcgttgcaat aataaaaatg gtaatgctat tattcatcat gtgcttcata
     1921 gaaaaagctt gtttgtgcgt aaggctgctg gaaaggtaaa tgaggaacag gtggttgcag
     1981 ctaatattga cattgttttt ttgtgcatgt cactgaataa ggattttaac attcatagat
     2041 tggagaggta tctgtcttta acatgggaaa gtggagctat cccagtagta gtacttacta
     2101 aatcagactt atgtgacaat gtgcatcgta aattacaagt agttaattct atagccttgg
     2161 gtgtagatgt agtagttacc tcatctatag agccagatgg ttataaacag atttctaagt
     2221 atatcaaatc aggctgtact gttgcattac ttggctcttc aggagttgga aaatcttcat
     2281 taattaattg tctgctagga ggagaatttt taaatacaaa ggaaattcgc atagatgatg
     2341 ataaaggaag acatgccacg actagacgag agctattttt aatttcaggt ggtggtattg
     2401 ttattgacac accaggtatg agagagttgg gaatgtggga tgtttcagaa ggtttagata
     2461 aaagttttgc agatatagaa gcttatttcg gaaaatgtca ttttcgtaat tgtactcaca
     2521 cctcagaacc aggctgtgct atctgggaag ctattgctca tcaagaactt tcgcctaaaa
     2581 gatgggaatc ctatcgtagg cttaaaactg aagcagtatt tgcagacaat aaaagcgaat
     2641 acttagcaaa aaaagaaaag aaatttaaaa atatctcaaa aataaataag acgattagaa
     2701 gataagtgta gtttttttgt tcattctatg agtctatata ctattaatgt attaaatttg
     2761 ccattaattt gttactttaa tgtgattttg atggcaaatt tggtttgtgg tattatttaa
     2821 agcaatttat taattttttt aataaagttt gattttttat atgatgatat atatatgtga
     2881 taatgtatac atatttgtta cgatagtctc ctttataaag aggtcttatg taagataaga
     2941 aaaggcatat ttatactaat acataataga gtatatgctt atgtatgata gagatgtttc
     3001 ttaagagcaa aactgcaata ttgctaattt ataaagtaaa aatatatgaa ttataaaata
     3061 caaataagat atttataaaa attattgttt taatttataa taaaaatttt ttgttgaaaa
     3121 caatgctcga acctataaaa ctattaacta atatttgtaa atgtatactt ttgatgataa
     3181 aatataatta agttaagttg gtttttataa acataaattt attttagaga ggtgaaataa
     3241 atgaaaatat ctagtcaata tagaagtcaa tattcattta gacatgaaag taatataaat
     3301 aatacaagaa taaatgaaag tatggttaag aaaaatgaaa ctgtaggaaa agacacttat
     3361 ttatctaata ttatgaaaca aaagcaagaa cttaatgata gaattagaga tttaaaatat
     3421 agacaagagg tttatactaa aaaaataaat gaagcaatta agaacttata taaatcagaa
     3481 ataagagaaa caactaataa cttttctaat atagaaatag gtattaaaaa tagcattata
     3541 gaagataaaa ataaaagtac aatgttagat gaaaattcaa cttatctaaa tacaaatgat
     3601 gaaaaagaat ctttaatcac taaagagtct aatgaaaaaa ttgaagaaga aatattaaat
     3661 gatgaaaaat tagaagagtt agaacaaaaa aaggattata aagaggattc taataaaaaa
     3721 gagaaagtat ctgaggactt atctttagta ggtaaaactc gtgaagagct tgaaaatatg
     3781 cttaaaaatt ttataaattt aacacaagaa gaaataatga aacttgagtc gagaatagaa
     3841 aagttagata aagatgctga agaatacaaa caaaattcaa agactaatat atttgataaa
     3901 acagatgaac aaaaaaaaca tataaatgta ctgatttaaa caatatgttt taaaaaaata
     3961 agtttaattt actctatgga ggaaatccaa atggagttga gcttcgagca gatggtaaat
     4021 attattatat aaataagggt atggaagggc taggatttta tgcagcagca ttgtcaggta
     4081 gctcatatca gagaataggc tttggaaaac ttgaccctat agaagtaata gccgatggag
     4141 attggatttc ttacaaacaa gctcaagata cacttacagt aatcaggaat tttttaaata
     4201 gttttgactg gagaaatgca tctgagatgg aaagagctaa tagagctgca aaattggtaa
     4261 ccgaagcaaa atatgtagac tctaaatatt gtaacatagt ctatggtaat ctggttgata
     4321 aacgtggtgt atgtggaagt tttgcaagta gctttcattt attaacaagg ttaatgggaa
     4381 tggattcact atctatatta aatccatcat taaatcatgc ttggaactat atacaaattg
     4441 atgggaaatg gtataggtct gatgggtctg aaatatcagc ttttggtgga gcattagact
     4501 ttgattaccg taaattaaag gatgcaacaa gagagatgac tacttactat gatgctaagg
     4561 cattgtctat attaggattt aatcaataaa aataggggag gctttataaa gagccttttt
     4621 ttattgattt gaaatttaat aaggaatttt atatgaaaat ttaatcaata aaagcaaaaa
     4681 aggctcttta taaagccttt ctaagtgttt ttttaagtaa aattttttat atcaacatca
     4741 gttttaatca aaaaatataa cattttggac ttacttttaa aaataaaatt attaaaatat
     4801 aatattaatc atgttaataa tgtttttagt ttattatatt ctataatagt ggtcatgttg
     4861 actattgtag ttttattaag aatagttggt ttatataaat atatgcaaag atagcttgtt
     4921 gtttacttta cttattagta tataggacaa gatactctaa taaatttgcc tattttaata
     4981 tcttttgtac taaaaccaac taaagtttcc tttattattt cgttttgaat aatacaaagc
     5041 ataatccgat tgtttgtata aagactggaa agtatcagat ttattttcta atttagatat
     5101 acctatagaa caagaattac tctctagaca taattctaaa actttctttg ctttattttc
     5161 aacattctct tttgattgat tactatattc aaaaacagca aattcatctc caccaagtct
     5221 tccaataata gcatcattta cgaatacttt agtcaaaata tttgaaaact gtattagtat
     5281 ttcatctcct cgaacatgac caaagatatc atttacctgt ttaaaattat ctaaatcaaa
     5341 gatgtaaaag tatccatatg aaacagtttt aagcatttct tttatgtgta cttcaaaaaa
     5401 atgtctattg tatagtccag tcataaggtc tttttctgat tttagtttaa attcatttgc
     5461 cattgctagt gcttcttgtg ctttttcagt tattgtttta gggtaatact catctttcat
     5521 ttggtcaaag tttggaatag aatggtgaac atggataagt ttaatcatat catctgtaac
     5581 tctgtatacc atagtaaatc gagtatccat ttctacatag atatcttttt ctaggtaatc
     5641 tttttctatt acccaaagag taccatatac tataaaagct tcgtctgtaa ctttggtaca
     5701 tgaataccac tcatctataa tctcaaagtc tatttgctct gattctgttt ggtctttttc
     5761 aagacctata ataaattctt ctaatgtttc gtaaaattca tgttttcctg ttccaattaa
     5821 tgcaaggttg ttatctaata aggaaataat aaatttaagg ttttcttctt ctggatttaa
     5881 aatataaata tgccaaattt tttttgataa atctcttaat tgtttttgta ttgtattatt
     5941 ttccatagat taccccctta agatttacag tttatatttt actgtattat aagttgaact
     6001 cttattacaa tattattata atatagaact aaagaaatta catcctgtat aggtaaaagt
     6061 atatctttta caataaaaat attacagtaa aataaccagt tttaatatac gtattatatt
     6121 tttaatttat aaacgaatat tataaatgaa atttaaattc aaccctgaag aaataagcta
     6181 aatgtattag tatataaatc catacttgaa cataaataac ctttatgata tgatatcaat
     6241 aataatatta tgagaggagt gccttgatgt cggagttata taatggtaac caacaattaa
     6301 atagttggag acacatatat tctgtataat cagaaatttt aagtgtattt ttaatacatt
     6361 taatttttgt gtcttattct gcctttatag ataactgaca tatgacaaca aatagtattt
     6421 aaaatatata ttactttgtt aaattatggt gtagtaggct ttaatctgct acacttttat
     6481 taattttgta tagtattatt ttgataacta ttttgaaata gagagcatta atttttctag
     6541 tccacaggag ttttctgttt ttgtgctgtt aaatataagc acagaaaaag gcagatatat
     6601 acctgtggac tttttttata taaattatga aatatggagg tagttaaaat gagtataatt
     6661 gaacttaatg aactgactaa atcttataga agatttgaga aaaaggcagg atttatgggg
     6721 agtgtaaagt ctttatttaa taggaaatat acagaaaaaa cagctatatc gtcatttgat
     6781 ttaaagatag aagaaggaga atttgttgga ttaataggtc aaaatggagc tggcaaaact
     6841 actcttatta agatgttaac tggtattatt gcaccgtctt ctggagaaat atctgttatg
     6901 ggttattacc ctaacaaatt agaaaataat tttaaaaaac aatatgctgt tgttatgggc
     6961 caaaaaagtc aactattctt tgaattgaca ccagcagata cgtttctttt atttaaagaa
     7021 ttatatgata tatctaatga agaatataaa aaaaacttgg attactttat cgaattattt
     7081 gaggtatctg actatttaaa tgttcaagtt aggacgcttt ctttaggaga aagaatgaaa
     7141 atggaattaa tagtagccct tctacataat ccaaaaatat tatttttgga tgaaccaact
     7201 ataggcttag atgctatagc ccaaaaacaa attaggtatt tcctaaaaga agtaaatgaa
     7261 acaaaaggaa ctactattat acttacttct cattacatgg aagatattaa gagtctctgt
     7321 agtagaagta ttgttgtaga aaatagccgt aaaatatatg atggttctac agagaaacta
     7381 tttaatcatt ttcaaaaata taaaaaaata acagttagtt tttccaatga aactaacatt
     7441 gtatttccaa aagattgtaa agttgttgaa caaacaaatc ataagacatc agtaattgtg
     7501 ctaaaagaaa gaggaaatga aacgattaaa tggatttttg ataaatatga tgttaaagat
     7561 gtaatgattg aagaagaaga tataggtagt attgtagaga gaatatataa gaaaggaagt
     7621 gatgagtatt atgagcaact ataatgaagc aagttcaaat tattcaatgt tttataaatt
     7681 taaacaaagt attagaaggt attatgaagt agccaaaatt aactttaaag tgcaacttat
     7741 ctggagattt gatgtggtgg ttaatatggt gtttacgatt gtaaaaattt tatttgctta
     7801 tattttatgg aaagcaattt ttggtgaaaa taaaatagtc tctggtttta catttaactc
     7861 tatgttgtct tactatataa taagctcatt tttagcaggt attgatatgt caaagagtac
     7921 cagtgaagag atatgtaatc gtgtaaaatt gggcacattt tcaaagtaca tgattattcc
     7981 aattaatgtt caaaattatt ttattgctaa aaattttgga atgacatgtt tttattttgt
     8041 atttaatatg atttctgcat ttgtatggat ttttatattt aaaatagatt ttgcctttac
     8101 atcaaatcca gttcaaatat taatttcaat tttgatgatt tttctagggt ttatttttat
     8161 gatgaaactc cacttcttta ttgggatttt gtcatttaaa tttcttgaga ttagtttttt
     8221 tagaatgata attgaaaata ttgttgaatt tattacagga acaatgatac cacttgtact
     8281 cttaccagaa actatagtaa cagttatgaa ggtatttcca ttttattata caacatattt
     8341 gccatctatg ttactaatag gtagaaacag caatgaggca atacaaggat taattattct
     8401 agcattatgg gttttatttt tttctgtagt aaataaagtt atgtataaaa gaatgagaac
     8461 attatttgat ggggtgggta tatgatgaaa aaaaatttga aaataatatt tatgttatta
     8521 aaaatgaaat tatcaaagat gatggtattt agatttgatt tttttggagc attttttgta
     8581 gatagttcat tatttatatt acaacttcta atgtttaata gtatatattc tcatgtagac
     8641 tcaatagggg gatggcaaca aggagagatg ttaatattta taggaacttt ctcattaata
     8701 aatgcgataa atatgacgat tttctttttt ggtatttatg atattccacg aaaaatccaa
     8761 gaaggagaat tagactacta tattacaaaa ccattagacc cactatttag gttaacattt
     8821 gaaaatatta atattggttc atcaccatta attctagcaa gtatacttat tgttttatat
     8881 ggaatatcta agttaaactc agagataact ttagttatga taataatata tattttcatg
     8941 gtgttattga tgacattact tttttatgat gtatgtatca tacttagaac agtttcattt
     9001 tttgttatat catcttctgc gataatgaga ttagaagata atttattgcc aatgaatatg
     9061 aaaattcctg gtgtaatata tgaaggtgtt tttaaagtat tattctattt aatcttacca
     9121 tatggaatta tgtcgacaat tccgactcaa attttggctg ggacaataac aattaatgga
     9181 ttaatatact caattttaat tgtatttttg tttacattgt ttatgtggag attttggaag
     9241 ttaggtatga gacattataa aagtgcaagt agttaataaa ttagtaaaaa tagacaatgt
     9301 ataactatat ataaatacgt ttcatatgac actaaactat gtgaacaagt atttgcgcta
     9361 aaggcactga aagctataaa atgattaaag taaggattta aaagtttctt tagtagaaat
     9421 ataattgtgt aaaaaagcaa gattaattaa aactatatcc aaataattag atataattct
     9481 tatgcagaaa ggaggtctgt aaatgtctaa ttgtttggaa tatattttaa aaataattaa
     9541 tgtacagaaa aataaacaaa atgagtgatg tatattattc agaaaatcat gaacaagata
     9601 atctttatga gcaagataat ttactggcac aaagatttaa tttatttact ctattgaagt
     9661 ttgcatttcc tactattttt atgatgattt ttatggggtt gtatacaatc gtagatacta
     9721 tatttgtatc aagatttatt aatacaaatg ctctatcagc aattaatatt gtatgtccaa
     9781 ttattaatct tatagttgga attggaacta tgcttgccac tggaggaagt gctattgtag
     9841 cacgtaagat gggaaatggt gatattgagg gagcaaataa agctttttct ttaatcgtat
     9901 tatcaggagt tgtaattggg gtaataatta ctggtatagg tattgtattt atggattata
     9961 tcattatatg gttaggagca agtgaagttt taattaaata ttgtcgttca tatcttttta
    10021 ttatcttggt ttttgcttct gcaaatatac tacaagtatt atttcaatgt ttatttgtaa
    10081 cagctggaaa gcctcaattt ggattatggc tagtgttagg tgctgggtgt gctaatataa
    10141 ttcttgacta tgtgttcatt gttctgttac aaatgggtat tagtggagca gcacttgcaa
    10201 ctggagtagg atacctaatt cctaccgttt caggtatggt tttcttttca aaaaataaag
    10261 agttacttaa gttttgtata cccagactag ataaaagaat attatttgaa gcttgtttta
    10321 atggttcatc tgaaatggta ggtcaactat caacagcagt tactactttt ttatttaatg
    10381 ctatgatgat gaaattaatt ggtgaggatg gagttgcagc tattacaata attatttatt
    10441 ctcaatttct tttaacttcg ctttatatag gtttctcaat gggtgttgca ccaataataa
    10501 gttataacta tggaagtgaa aataatacac agttaaatca tgtttttaag atttgtatat
    10561 tatttatttc agtggcatct ttattggtat ttatcttttc tatatcatgt tcaccaatca
    10621 ttgtagagat tttttctcaa agaggaagcc gagtttatga aattgcaagg tctggatttt
    10681 atatattttc ttttagtttt attttttgtg ggtataatat ttttacttcg tctatgttta
    10741 cagcattgtc aaatggcaag gtctctgcaa tattatcttt tctacgtaca tttggattta
    10801 taactatagg tttattgata ctgccaaata tattaaatat aaatgggata tggttagcag
    10861 taccaagtgc tgagatttta actttatttt taaccatttt ttgtatatgt aagtataaga
    10921 atagatataa atatatgtag atattaaata aatagtttag gtagatattt tatgttttaa
    10981 taagtaattc aattagtggt agatatatac tattgaaaat taaagttata attgatataa
    11041 agaattaata aaaataacat ataaaagttt aaaagggagt tgaacttaaa gtttgattag
    11101 aaaaagtcaa ttttaagaca actcctttta ttttactatt gtattaatct gtatctgtat
    11161 ctgtaagtgt attagtctaa tattataaac tttggatgaa attaattttc tataggtttt
    11221 aatgtatcat acactttatt ttaaagaaac ttatcttaat tttcatagtg attgaatttg
    11281 tctattagag atgttactat tagtaatgta aaagcaagag tagtgaatat aaaaaaacaa
    11341 gatattgata ctgcaagatt agaatataat aaatttttat ggagagattt caatatggat
    11401 acatatttta ataaaaaaat attacttgtt gatgatgaaa aagatatagt agatttaatt
    11461 gaagaagtac tcataaatga tggttttaaa aacattatta aagcttataa tggtttagat
    11521 gctatttcgc tgtgtaagat agcatgtcca gatgttgtca ttcttgatat aatgttacca
    11581 gatatagatg gtatagaagt gtgtaaaaaa attcgagagt tttcatactg ttctattctt
    11641 tttttatcat caaaaaatga tgatattgac aaaatactag gtcttagtag tgggggagat
    11701 gactatataa caaagccatt cagtccaaga gaaattgctt ttcgagttaa agcgcaatta
    11761 cgtagacaac agtatcaaag cattgtacca tcagatagtg cagttattaa aataggagat
    11821 attactattg atatagaagg aaaccgtgta tataaagata gaaatgagat tgagttaact
    11881 ggaagggagt atcatctttt aagctacatg gctcaaaatg taaataaaat tataggaaaa
    11941 gaaagactct atgaacaagt atggggagta tatagtagta tttgtgacaa tacaattatg
    12001 gtgcatatac gacatataag agaaaaaatt gaagataacc cctcaaatcc taaaatactt
    12061 ataacggtta aaggactagg atataaactt gtgaatagaa tcgattagaa tagattttaa
    12121 tattaatgaa gatgtctcaa gttgaatttt tagttaatga ggcattcttt tatgtgtgaa
    12181 gtcaaatgta ttttcgttat ctcaacaatg aagaagaggt ctatctctat tttgagatag
    12241 cctcattaag tttatatcct ggtaatgtac tttatgttct aataaaattt ttttagttta
    12301 tttgcagtga aacattaact agaacattaa aatttgcatt gaaatatcaa ttaaataata
    12361 ttgtatattg tgtataaact tatactaatt gtttttctat attaaacact ttatcaatcc
    12421 aacctttata aaatttttct tcatgggaaa ctaggattat acttcctcta aactgtttca
    12481 aagcattttg taaagcatcc tttgtctctg catcaaagtg attggttggt tcatccagaa
    12541 taagaaaatt acaaggagat aacatcatac aacataattt aaccttagat tgttcaccac
    12601 cacttaaagt agatacactc cttgaaacat gctcttcctt cacaccacat ctggctagat
    12661 gacgtctaat ctcttttgta tttaattttg gatatttatc tgctactatt tgaagtgggg
    12721 ttttatcagg attttcccat tttaaatctt gctcataata accaatttta acttgttctg
    12781 aaaactggaa atctccagat atacgaggaa tatctttgac taatgtcttt aacaaggtag
    12841 atttaccgat tccattaaaa ccagttataa ctattttttg acctccatca acagaaaaat
    12901 ttaattttgg aagtaaagaa tagtagtatc caacttcaag atttgttata gtaagagcat
    12961 tttgagccga tatttcaatt tctgaaaatt gaatatttgg ttttcctgta aaactaggag
    13021 gagctattct ttctatttta tccaattgtt tttgtcttcc tcttgcaatt tttgaattta
    13081 ctccagcttt atttttacgt ataaatgtct cttctttttc tattttcttt tgttgtgctt
    13141 gataccttct aatataatct tctcttaaat gtgatttttg tttaaggaac tctgaatatt
    13201 ttccatgata ttttttaatc ataccaaatt ctatatcaag aatacctgta gaaatttttt
    13261 ctaaaaaatc aaaatcgtgg gaaactacaa taaatgcccc attaaatgta tttaaataat
    13321 ttgagagcca ttcaacatgt tctttatcta aaaagtttgt tggttcatct aataatagaa
    13381 catcatggtt agaaagtagc aattttgcta aaattacctt tgccctttgc ccaccactaa
    13441 gctcttcaac aacacgattc attcctattg aatctaatcc aagaccattt gctattttat
    13501 ttatttcatt atctatagaa tagaaattac ttgctattag ttgttcctga taatctgatg
    13561 ctttgattag atattgctca ttttcagata tagcactttt ttgatagagt aaattcattt
    13621 ccttttctat tttataaagt tcttcaaatg cagtgtgtaa atagagtgat attgttgtat
    13681 ctctattgat ttctgcatat tggtctaaat ggcctatatt gatattaggt tgccacttga
    13741 tttctcctga atctggaaca acttctccca ataaaatttt aattaatgta cttttacctg
    13801 taccattttg gccaactaca cctatatgtt cacctttgaa taaatcaaag ttagctttct
    13861 catacagtga tttatccata aaacattgtg ataaattagt tacttttaat aaactcataa
    13921 ttcaaactcc ttttctttta ttaaaaaatt tatggaaata aaaaaagcag aggaatggca
    13981 ttgtaaaatg ccgacctctg cttttggaaa aacaaaaggt catagacaag cattgttgtc
    14041 tatgaccaga aaatctatac tatctatcgg caaataggta attttaaata agcatagcca
    14101 aaaaactata ctaaacctat atttgccgat gaagatagaa agctttaagc tttcagatta
    14161 acctctacac aacgcttcat cgtatacatt tgtgtagagt taagttttct aacaagttta
    14221 actaattcta tataaatcat aattattcac atcctaattt tagtattaat tctagtttat
    14281 ctatatttaa attgtacttt tgtactttgt ggattatctt atttatttaa aatatgtttt
    14341 attaattata gcttaagtta tgttatcata atacttggtt atagtcaata agaaagttat
    14401 atttgctata tattatatta atttagtaaa tctatcatta caaagttatt tgaagactcg
    14461 ctaccaataa aattttatag tctatgggaa aattaagatt atgagtttta tacttttgat
    14521 atagggatac tattttcata aatattacaa catgtagaag tatatgtcta gtattgttga
    14581 attttcaatg atatattaag gttctaatat attatacaaa ataaaaacat taattcaact
    14641 taaataattt taagtattat atgtgttttg attaaagaaa actaaaattt taaggaaaaa
    14701 taacatattt gtattaaatt ttattgaaat gattaaattt atttacttaa tgtaaattat
    14761 tagctaaaat tgtattaaat aattaaggag gtgagtagct ctggatgaaa tagatttaaa
    14821 aataattaat tctctaaaaa agaatagtag ggcttcaacc tctgaaataa gcagacaaat
    14881 taatttatca gtaccagcag tctcagagcg tataaggaaa attgagcagt caaatttaat
    14941 tcaaaagtac actattcaaa ttaatcggga aaaaacaaaa tataagcttt tggcttttat
    15001 atttatcaat actgatttat ctaaaagtat gaaagaattt aagaaaacta tattggaata
    15061 tgatactgta ttagaatgtc atcatatttc aggagagtat gattacttaa taaaagtatt
    15121 agcagaagat acaagtaatc ttgaagagtt tatatcagtg actttgaagg aaattggggg
    15181 gattcaaaaa gctaatacaa ccattgtttt atctacaatt aaagaagaat ttaatacaat
    15241 aaaagatatt ttttaaataa tttactagta aagtgttaat tatattttag gaggaaaata
    15301 gaatatgaaa gttttagttt ttttagcaaa aggttttgaa actatggaat ttagtgtatt
    15361 tgtagatgtt atgggatggg ctcgtaacga ttatgggcat gatattgatg tagtgacttg
    15421 tggatttaaa aaacaagtta tgagtacatt taatatacaa gtattagttg ataagacgat
    15481 agaagaagta tgtgttgatg attatgatgc cctagctatt cctggaggat ttgaagaatt
    15541 tggattttat gatgaagcat atgattcatc ttttttaaat ctaataaggg agtttaattc
    15601 aaaagaaaaa ataatcgctt ctatttgtgt ggcagcattg ccagttggaa aaagtggtgt
    15661 tttaaaaaat cgtaaagcta ctacatatca tttgaaaaat ggtaaaagac aaagacagtt
    15721 aagtgagttt gatgtaaatg tagttaatga accaattgtt gtagataaaa atattataac
    15781 ctcatattgt ccagaaacag ctccacatgt agcttttaag cttttagaaa tgttgacatc
    15841 tagagaacaa atggatgaag ttaaattagc aatgggattt aaactgtaat gctctataga
    15901 ctttctaagt aatcagaacc ccagtcacac atggaatcta aaattggaat aatactcttt
    15961 ccaaaatcag atagcgagta ttctactttt ggaggaacaa caggataaac cactcttata
    16021 attaaaccat ctgattctaa ttcacgtaat tgttgagtta acatcttagg cgttgcttta
    16081 ggaattaatt tttttaattc attaaacctc aatatagtgt ctttaagatg ccaaagtata
    16141 agtgtcttat atttaccacc aatcaatgca agtgttgctt ctattgggca actgtaatta
    16201 tctaaacatt ttttcatttt tgagctcctt tatttatttt aaagtatctt tttagatact
    16261 atattacaaa aaagtgcatt attgtaatat agtaccatag tgataaaatt atagcatagg
    16321 ttaaataaat catcaactta gcctaataaa acaatagggg gtaaataaat gaatttcgtt
    16381 gaacttacaa aaaaaagata ttcttgccgt aattatcaag atagaaaggt tgaaaaagaa
    16441 aaacttgaga aggttttaga tgttgcaaga atagctccaa ctggtggtaa tcgtcaacct
    16501 caaagattga ttgttattca ggaaaaagaa ggtataaata aactttctaa ggctgccaat
    16561 atatatgatg ctccattagc aattcttgta tgtggagata aagacaaagt ttggacaaga
    16621 ccttttgatg ggagacaact tacagatatt gacacaagta tagttacaga ccatatgatg
    16681 ttacaggcaa ctgaacttgg attggcaagt gtttgggtgt gctattttaa tccagatata
    16741 attagagaag agtttagttt gccagataat ctagaaccaa taaatatatt attgatggga
    16801 tatgaatcta agataccaga gagcccagag agacatgaaa aaactcgtgt tcctttaagt
    16861 gaaattgttt cttatgaaac tctttaatta taaaattaag ttattcatag gataaatgta
    16921 ataaagtgtt tattataaac aaaattcaaa ataaataata tctacatata attaatttta
    16981 gtgaagtata tctagtttaa ctagaatgta tacttcacta atttatttat tcaaaatatt
    17041 tatgaggatt ttaatatatt aactaaaata tatcaattga attaactttt aaatctggaa
    17101 aagcacttga aatatagtgt tttatgtcat ttaaatggtc ttgaatttgc ttttctgata
    17161 ttttatcagg tccatataca aaatacataa tatttttagt atcttttgtt atagctgttc
    17221 gattatcagg gccattaaga atactagata atataccaat attatctgat aaaaataaat
    17281 catcttttgt taaaatatgc tctttaccac ctataccata aaaatgttca cttccatttg
    17341 ctaattctat tttcaaaggt aattccattt tatcaaggtc atgacctgct gttagaagaa
    17401 gatttttcaa ttctgctaaa aacattgact caattgttac acctacatct gggatacctt
    17461 ttgattttaa tagaatagat tctaattgaa gcaatacagg gtaagttttc ttgaattttt
    17521 tataatactt tatataactt gaaacaggct ctgttttaat tttttcttta cgattgtatt
    17581 ctgggtgttg ttctataata tttttaattg tagagttttt aagaactaaa aaattttctt
    17641 tattaggttt tgaatgtgta atatttataa ccataactcc aaatttaata ttgggataaa
    17701 tttctttaac tttatgtgat atttctaaca taatatagac tcctttttat tttatatttt
    17761 atgatataat cttagcaagt taaaagtgga ttttgtagtt ccatttttat agtaatttta
    17821 tagaaccact tttggaggta ttagaatgtg gattacgatt aatagaaata gtaatatatc
    17881 attatcaaga caaatttatt gtgagataaa agatatgatt ctggatggaa gcttaaagtt
    17941 tgagcaaaaa ctgccatcat ctaggacttt atcaaaagaa ctatctgtat ctcgtaatac
    18001 agttttagat gcatataatc aattaattgc agaaggatat ttggaaacaa ggaatggata
    18061 tggaacaatt gtagctaaag gaatatcagg gcataaagtt gattttaagt taaatgatac
    18121 ttttagagat gaaaaagatt tacatactaa taaacaaaaa aattatatag attttcgttc
    18181 aggagttcca gaattaaaac tatttccacg caaagaactt tcaaagctat ataaacaaat
    18241 actgtatgat ttatcagatt cggatttcag atatggaagt acagctggtg tatgggtttt
    18301 aagagtggaa atatctaagt atttatttcg tactagagga attagatgta attccaaaaa
    18361 cattatgatt gtttctggtt caacacaagg tttatcttta atttcacagc ttttatacaa
    18421 aagtaataaa aaggttgctg tagaagaccc tattcataaa ggcttattaa aggttatttc
    18481 atctattgga tattcagttg ttggaattaa agtagatgat aagggaatga ataccaactt
    18541 aataaaatca ataaagggga tttcttttgt ttatactaca ccttctcatc aatatccatt
    18601 aggagggatt ttacctattc aaagaagact agagcttatt aaatgtgcaa cagagaatga
    18661 ttactatata attgaagatg attatgatag tgaatttaga tatgagggtc aaccaattag
    18721 ttctctttat gaacttagcc caaataaagt aatttacata ggttctttta gtaaaatatt
    18781 atctccagct attcgtgttg gatttatgtt attaccagac aatattttag aaagctataa
    18841 aaccttaaaa atgtactctg atgttcatac agaaacaata ttgcaatatg tattagctga
    18901 atttattgga aatggaggtt tagagaagta catatttaga atgaagaagt tgtacaatag
    18961 aaaacgtaaa tatctcataa aagttttaaa cacggaattt aaaaatgaat ttaaaataaa
    19021 aggtgaagca gcagggcttc atatagttgc tcatttctat aatgttaaat ttacggaaag
    19081 tttaattcat aaaatttttg aagcaggtat aaaagtttat cctatacaac aatatgcaat
    19141 tgagaatata ttagaatatg accatgaaat tatactaggt tatgctcatc ttagttttga
    19201 tgaaattgaa aaaggtgtaa aaatactcag caaaataata catatgaatc aaggtacata
    19261 gataaaacat gtagcaatct acatataata gatgtaatta agtttaaact ttaaaagaaa
    19321 tatatattaa tatttttgta taaaaatact tgatattgtt ttatcgatac tttattgcta
    19381 tacactaaat taagatagca atttatatta agaaaaaact ttttgcatag gttataatac
    19441 ttaaaaggag aggatagagt tgaaaacaaa tacagatgtt attatggaga taattgatta
    19501 tatagaagaa aatcttacag aaaatctaaa tttagataaa atagcttata ttgctaatta
    19561 ttcaaaatac catttacaca gaatgtttac aaatgttgta ggatgtacta tccatcaata
    19621 catacagagg aggagactta ctgaagcagc tagacaactt atatttacag acaaatatat
    19681 tatagatata gctattacag taggatattc tacacatcaa tcttttacac tagcatttag
    19741 aaatttatat aaagaatctc cacaagtata tagaaataaa gatgaatttt atcctattca
    19801 acagagattt gttttaaatc taaatcatca aattcaaagc gaaaaatgta tttcttatgc
    19861 aaatatgaat tataaacaaa gaagagatga aatcaattct tatcttaact ttaatattaa
    19921 ttgtgaggtg aaagtagcat gagtttagtg ccacatgtaa ttgaacaaac aggtcaaggg
    19981 gaaaggtctt atgatatata ttcaaaatta ttaaaagata gaattatatt tataggagaa
    20041 gaaattaatg atgctattgc aagtttagta gtagctcaat tactgttttt agaatcagaa
    20101 gacccagatt cagatataat tatatatata aatagccctg gtggctctgc tacatcagga
    20161 tttgctatat atgatactat gaattatgtc agatgtgatg tatcgactat ctgtattggt
    20221 atggctgcaa gtatgagtgc ttttttactt gcaggaggaa cacatggaaa aagatgtgct
    20281 ttacccaatt cagagattat gattcatcag ccaatgggag gagctaaagg tcaagcaaca
    20341 gatgtaaaaa tagctgtaga taatatattg aaaataaaag aacgattaga taaaattctt
    20401 tctgaaaata caggtaaatc aatagaagat attagaagag atacagatag agataatttt
    20461 atgactgcac tagaggctaa agagtacggg ctgattgatt atataatgaa taggaatgaa
    20521 taggaatgaa taaagtatca aagtatcata ttaaaatgtc tgttttacaa atatatatat
    20581 aatattaaat aagagtgtct caaaatgaac tttttagttt atgggacact cttttttgta
    20641 tgaaattaaa tatggtttta tcatttcaac aattattacc aaatttcaag tttaataaat
    20701 tctactataa aaaatacata aaagataagg ttctttagta actttctgtc gtcaaaagtt
    20761 tttataaatt taataacaaa acaagagaaa ataccatata ttacttatgt ctgcaatata
    20821 tagaagacaa aggagttgta cattatgatt gcaataagtt tgtgcatgat tgttaagaat
    20881 gaggaagaag ttataggcag atgcttagaa tgtgtaaagg atatagtaga cgaaattata
    20941 attgtagata ccggttctac agattccaca aaagaaatag tctcaaaata tacagacatg
    21001 gtgtacgatt tcgaatggat agatgatttc tctgctgcaa gaaatttttc attttctaaa
    21061 gcttctaaag actatactat gtggcttgat gcagatgaca taattttaga agctgataga
    21121 gaaaaattat taaagattaa agaaagcttg gatacttcca ttgatattgt tatggcaaaa
    21181 tacaatgtat cctttgatga aaatggtaac ccaactctct catattatag agaacgattg
    21241 tttaaaagaa gttctaatca taaatgggtt gaccctattc atgaagtagt acctcttttt
    21301 ggagaggtat tttattctga cattgcaata tctcataaaa agcttcatag acaggaccct
    21361 ctaagaaatc ttagaatttt tgaaaaaatg atttcagaag gaaaaacatt ggagccaaga
    21421 caccaattct attactctag agagttgtac tataatgcta ggtataaaga agcaatagaa
    21481 ggttttacta agtttttaga ctcatcaaga ggatggattg aggattgtat cagtgcctgc
    21541 agagatttag ccacatgtta ttacttaatt aatgatgaaa aaagtgcctt atattcattg
    21601 tttagaagtt tcgaatttga tgagccaaga gcagaaattt gttgtgacat aggaaagcat
    21661 atgtttgata gacaaaaata taaagaagcc attttttggt ataaggtagc attaacaaga
    21721 gataagaatg atactaatgg aggttttaag tcaaatgatt gttatggata tataccatct
    21781 atacaattaa gtgtctgtta tgacaggtta ggagaatcag ataaagctat ttattatcat
    21841 gaaaaaacaa aagaaatcaa acctaacgat agtgctgttt tgcacaatga aaattatttt
    21901 agtaaattta aaaatagttg ataggtcaca atcatactaa atttgacata ttatatatag
    21961 agcttaaaag ctcaaatata tcaggaggtg cttttaataa tgagtagaaa taaatatttt
    22021 ggaccatttg atgataatga ttacaacaat ggctatgata aatatgatga ttgtaataat
    22081 ggtcgtgatg attataatag ctgtgattgc catcattgct gtccaccatc atgtgtaggt
    22141 ccaacaggcc caatgggtcc aagaggtaga accggcccaa caggaccaac gggtccaaca
    22201 ggtccaggag tagggggaac aggcccaaca ggaccaaccg gtccgactgg cccaacagga
    22261 aatacaggga atacaggagc aacaggatta agaggtccaa caggagcaac agggggaaca
    22321 ggcccaacag gagcgacagg agctataggg tttggagtaa caggcccaac aggcccaaca
    22381 ggagcgacag gagcaacagg agcagatgga gtaacaggtc caacaggtcc aacgggagca
    22441 acaggagcag atggaataac aggtccaaca ggagcaacag gggcaacagg atttggagta
    22501 acaggtccaa caggcccaac aggagcaaca ggagtaggag taacaggagc aacaggatta
    22561 ataggtccaa caggagcgac aggaacacct ggagcaacag gtccaacagg ggcaatagga
    22621 gcaacaggaa taggaataac aggtccaaca ggagcaacag gagcaacagg ggcagatgga
    22681 gcaacaggag taacaggccc aacaggccca acaggggcaa caggagcaga tggagtaaca
    22741 ggcccaacag gagcaacagg agcaacagga ataggaataa caggcccaac aggggcaaca
    22801 ggagcaacag gaataggaat aacaggagca acagggttaa taggtccaac cggagcaacc
    22861 ggagcaaccg gagcaacagg cccaacagga gtaacagggg caacaggagc agcaggacta
    22921 ataggaccaa ccggggcaac aggagtaacc ggagcagatg gagcaacagg agcgacaggg
    22981 gcaaccggag caacaggtcc aacaggagca gatggattag taggtccaac aggagcaaca
    23041 ggggcaacag gagcagatgg attagtaggc ccaacaggtc caacaggggc aaccggagta
    23101 ggaataactg gagcaaccgg agcaacagga gcgacaggtc caacaggagc agatggatta
    23161 gtaggtccaa ccggagcgac gggagcaaca ggagcagatg gagtagcagg tccaaccgga
    23221 gcaacagggg caacaggaaa tacaggagca gatggagcaa caggtccaac aggggcaaca
    23281 ggtccaacag gagcagacgg attagtaggt ccaacaggag caaccggagc aacaggatta
    23341 gcaggagcaa ccggagcaac aggcccaata ggagcaacag gtccaacagg agcagatgga
    23401 gcaacagggg caaccggagc aacaggtcca acaggggcag atggattagt aggtccaacc
    23461 ggagcaacgg gagcaacagg ggcaacaggt ccaacaggcc caacaggtgc tagtgcaata
    23521 ataccttttg catcaggtat accactatca cttacaacta tagctggagg attagtaggt
    23581 acaccaggtt ttgttggctt tggtagttca gctccaggat taagtatagt tggtggagta
    23641 atagacctta caaacgcagc agggacattg actaactttg cattttcaat gccaagagat
    23701 ggaacaataa catctatttc agcatacttc agtacaacag cagcactttc acttgttggt
    23761 tcaacaatta caattacagc aacactttac caatctactg caccaaataa ctcatttaca
    23821 gctgtaccag gagcgacagt tacactagct ccaccactta caggtatatt atcagttggt
    23881 tcaatttcta gtggaattgt aacaggatta aatatagcag caacagcaga aactcgattc
    23941 ttactagtat ttactgcaac agcttcaggt ctttcattag ttaatactgt agcaggatat
    24001 gcaagtgcag gaattgcaat aaattagatt agataagaaa aggcttctaa tatcgtattg
    24061 atattaggag cttttttaag aatgattgat gaacatagtt ttaatattaa ttagtgcgca
    24121 tattataaaa tactattata taatataaaa taataacatt aaattggaaa tatagaatta
    24181 tctaggggtg atactatgaa aaataagatt gaaacatcaa tgaacaactt tgtaaaaaat
    24241 tatcataaat caaataatat aactacaaag tggcaagaac ttttgtttgc ttatgcatct
    24301 gctgaagatg atatgtttca gaagctaaaa agtgttgtag gaacatctca tgctatgcca
    24361 aaagattttc tggaaaatgg aaaaactata attacatatt ttataccttt tgatgaaagt
    24421 gtaaataata gtaatattgg agggtttgaa tgttctgaaa tatgggcaag agcgtacata
    24481 gaaactaata tgttgatttc aaatttaaat gatttcttat gtgaagaact agagaagatg
    24541 ggggctaatt catatgctat tcctgggtcg tataattttg acagtgaaaa attggtaagt
    24601 gactggtcac aaagacatgt agcattcata gcaggtcttg gaacatttgg tttaaataat
    24661 atgcttatta cagaaaaagg gtgttgtgga agaataggaa gcataatcac aagtttagaa
    24721 ttagagccaa ctaaaagacc agaaaatgaa tactgcttgt ataaacataa taatagttgc
    24781 aaaaaatgtg tcaacaaatg cgtgaaagaa gctttaaaag aagatggatt taataggaat
    24841 aagtgttatg aaatgtgtat gataaatgaa aaaaaataca cttatatagg tggttgtgat
    24901 atttgtggaa agtgtatagt tggggttcct tgttcggtaa taaatccctc aagataaagg
    24961 acttatgttg attaataact ttaaatatat ctaataagaa gaaaaaagtt agtctctaaa
    25021 atagatgaat tatgaaaata aataaagata atataaaggt tagatgaaga agttatgtta
    25081 attgacaact atttatactg ctgatagact tatatgtatc gttgcaatat ataaggaggg
    25141 gtgagattta tgtttgattt aatctttatt tttgcatagc aagggctatt gcaaatataa
    25201 aatttaatat ataaatagcc ctttgctatt cctaataaaa aattattatt taataggagt
    25261 gcagaatgag ctataaaaaa gcaatacata tattaccaga agaattactt gaattgatac
    25321 aggaatatgt agatggagaa tatatctata ttcctagaaa gtctaataat aaaaaagaat
    25381 ggggaagtag tacgtctact cgtgaagatt tatcaattag aaatatgcaa atttatgaag
    25441 attatcaagt tggatatgat ttgaaatatt tatcagaaaa atattacttg tctttgaaaa
    25501 gtattcaaag aattgtatta caagaaaaaa gaaaagatag ttaagaatga gccagtatag
    25561 ttttataatt atactggctc attttctttt gataaaagtg tttgaggtat agtttcttat
    25621 aagtggatgc tataatttaa ataatttcaa taacttgtac agcagataac gaatagagtt
    25681 gtaaagtatc taataagaag tagtagcttt ttatatgttt gaaattaaaa tttattaaaa
    25741 ttaggaggtc ttgttttgaa aaaaatactt gattatattc caccttttca taactttcta
    25801 ttaatttagt aatcttatag aaaggaagtt gcatttatga aaaaaattac ttgggaggtg
    25861 gaatatatct cccagttacc tgtaattagg tattgtaaga aatgtggaaa aaagactgaa
    25921 tatatttgtt caggtttatt tagggttaat gctcaacata aatatttgga tatatggtta
    25981 atatacaagt gttcaaattg tgattcaaca tggaactcaa ctatatattc tcgtgtcaat
    26041 ccaaaaagct taaatccaga aacactagaa caattccata caaacgatta taatcttgct
    26101 aagcaatatg ctatgaatac ggaactttta catagaaatg gagcagaagt tggtattcca
    26161 gagtataaaa tagtaggaca agaaattaat attaatgacc ttacacaagt tcatattatt
    26221 agtaaatatc catgtcagtt aaaggtatca acattattac gtgagaaact aggtttatct
    26281 aggaaggtat ttgacgaaat gttggattgt gggattatta gaagtaactc aggagttgat
    26341 ttaaagaaag gtaaattaaa ttatgaaatt atgttagata taggcaagga gaagcaaaag
    26401 tcttcaaatt gattagaata actaacttaa tatattttca ggaacatcat ttaaaaaaat
    26461 agtggcttta aaaaatttaa agccactatt ttttaatatg ttttatatga atatttaaaa
    26521 tatagtgtgt attcttcaaa attatagcga taattaaaat attactataa aaaggaaggt
    26581 gcatactttt tgtttaacaa aaactagata ctaaaaactt
//
LOCUS       DAMJWP010000029        26619 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.030, whole genome shotgun sequence.
ACCESSION   DAMJWP010000029 DAMJWP010000000
VERSION     DAMJWP010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26619
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.030"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(216..629)
                     /locus_tag="PC700_001088"
     CDS             complement(216..629)
                     /locus_tag="PC700_001088"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDF2580408.1"
                     /translation="MNIALVAHDQMKNTMVGFCIGYESILKKYGLYATGTTGKRIMDE
                     TELNINRLASGPLGGDQQIGSLIVTQEIDLVIFLRDPLTSQAHETDIQALIRLCDVYH
                     VPIATNLASAEIFIKALDRGELSWREVRKSKSQRI"
     gene            complement(670..1800)
                     /gene="rodA"
                     /locus_tag="PC700_001089"
     CDS             complement(670..1800)
                     /gene="rodA"
                     /locus_tag="PC700_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HDF2580409.1"
                     /translation="MKKININYKSTIKLIKQLDWKLIVTVLAIFIFGLVILSSATHAN
                     STGSYNQLIKQGLAFVLGIGMIIVILFFDYNLLGRYYKALYIISLILLAIVLLPGIGT
                     VKGGARSWINLGPLDLQTSEIVKLTFVLSYAKILESKKDKLNTLKEVMPVVVYSLPFI
                     GLLIAQPDLGTGIVFCCMIFAMLFTAGLSSKLIKRGIIILLVSMPLMYLMMADHQKVR
                     IEAFLNPEDVTLKGNYQVMQSLIAIGSGGVTGKGLYNGSQNQEDFLPVQDSDFIFAVV
                     GEELGVIGMAVLIILFMIFLLRLLAIARDAKDFYGTLIVVGVMGMFGYQIIQNIGMTV
                     ALIPVTGVTLPFVSYGGSSLLTSLANLGLVLNVCMRRKKINF"
     gene            complement(1885..2169)
                     /gene="minE"
                     /locus_tag="PC700_001090"
     CDS             complement(1885..2169)
                     /gene="minE"
                     /locus_tag="PC700_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HDF2580410.1"
                     /translation="MLDLFRVFSNEAKTSKSVAKERLKLVLVHDRVDCSPQLLEMIKT
                     DILKVIANYAEIEDDGLEIKMSKCRGEHDDKPVSALVANIPLKNIKDRCM"
     gene            complement(2187..2984)
                     /gene="minD"
                     /locus_tag="PC700_001091"
     CDS             complement(2187..2984)
                     /gene="minD"
                     /locus_tag="PC700_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HDF2580411.1"
                     /translation="MSEVIVITSGKGGVGKTTTAANLGTALSLENKKTVVVDADIGLR
                     NLDVVMGLENRIVYDIVDVVEGTCRLKQALIKDKRFDNLYLLPAAQTRDKNAVSVEQM
                     IDLCEKLKESFEYIIIDCPAGIEQGFKNAVAGADRAIVVTNPEISAVRDADRIIGLLE
                     ANEIKEIRLVINRIRNDMVKRGDMMDKQDIIEILAIDLLGLVPDDESIIISTNKGEPA
                     ILDSKSLAGQAYKNIAKRILNEEVPLLDLEVEDGFFGRLKKMFSMAK"
     gene            complement(3008..3691)
                     /locus_tag="PC700_001092"
     CDS             complement(3008..3691)
                     /locus_tag="PC700_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HDF2580412.1"
                     /translation="MSLREICSQELVEFKGNKRGIIVNIKREAPFEEIQEKIINKLEA
                     YVGFFNGAKISKINSDCLTDMEILELKEGITSRFDVEFVEDQKIEENSNFPTKYVNTL
                     RSGENIEFEGDVVILNDMKPGSKVLSKSNTVVMGDINAGAKVVAGGNVFVMGKIEGFV
                     HAGAEGNEFAYVVAGNLNPKILQIADNIAEAPDDEENYENESEISPEIAFVSNGRIVI
                     ESYLSKLDK"
     gene            complement(3789..6767)
                     /locus_tag="PC700_001093"
     CDS             complement(3789..6767)
                     /locus_tag="PC700_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDF2580413.1"
                     /translation="MEENKQVDRLKIIKIVMSVIFLAILVKIIYMTTFKYEYYNELAE
                     NKTYKKLAIEAPRGEIKDRYGRLLAGNKNLFTVQVSGNDINKKDANKHSRANEISLKL
                     INLLERNGEEYVDEFPIYVENGKYYYTYDRDIREYKSENGIPNDYNAKESFYYLVDKL
                     ISAGILSQEDKRLDATRLQAKLNENGYYPPILVSKWMFTAERDKRDWLASYKIKETKL
                     SAKEAFEKVRNSDALEIDKSLSDEDARKIMVVRDLIKSKGYSQYNPVTIAKDVGETTI
                     AQIEESAMDLVGVSIAVEPVRYYPNGSLASHMLGYVGKMPSTQIESYLQKGYETGDMV
                     GLAGVEKSNESRLRGTDGYKMVKVDALGRISKEIESKKPKSGDTVYLTLDKDLQEVSD
                     NALKQIIEVASKGGTFKSKFGDKPISAYAGKAQSAALIAIDVKNGEVLASSSYPNYDP
                     NKFAKGISTEDYKALQPKNPNDLLAGSPLLNLVTQGEFQPGSSFKMLTSMAALENGLD
                     PNFTINDPGVIMLGKKSFGDYVWNHGRGNHGMTNLYKAIQESCNIYMATIGTGKTWPD
                     GKSIGIDMNANKILEYAKLFGLDQNTGLQDEVEERAGKVPSTEDKLKSTQALLKSNLE
                     REMANDFVDITREKNPKEYEKRINEIVSWAAEKKTPGRVETMNRLKKMNVKEDRIEEV
                     ADLAVFSYFNFAKWSTADTFNLAIGQGENAYTPAQISRYVAAIANGGNLVELSVVDRA
                     VSSDYSSVKINDQKKVEKIPFKNPDNLKELTKGMKLVARQGTAKSAFADFPIDVAAKT
                     GTAEKSGKIPTDNEYEYLKSHMSSYNVNLNDAIKLADKMKAEKEKELSLAKEKEIKKK
                     LENKDLKDEERKKLEEELEDGVKVRLEDTDKVNSSYLRKAIKELNPKITDDQIDRFKQ
                     DYGSFTWTVAFAPADDPEIAVVCVIPQGDSSVFSLLPTREVIGTYMGLEPTNSKNDNK
                     TNDVNNSSDENINFESQINR"
     gene            complement(6779..7267)
                     /gene="mreD"
                     /locus_tag="PC700_001094"
     CDS             complement(6779..7267)
                     /gene="mreD"
                     /locus_tag="PC700_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDF2580414.1"
                     /translation="MKKVLLCLLGIVLMTIENSITNYIDIFGISFNLVLIYVTIISLY
                     LDELEGGIIAAIIGLIKDITVGGIFGVNALILFIIAYAIGYMRDQLYKGSYIITFVLI
                     FIGSLIDSIVNIGTSSIIYQSYNISTLFVKGLLVAPIVNSLIGLVIYRLSKRAVLKLK
                     ED"
     gene            complement(7282..8175)
                     /gene="mreC"
                     /locus_tag="PC700_001095"
     CDS             complement(7282..8175)
                     /gene="mreC"
                     /locus_tag="PC700_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDF2580415.1"
                     /translation="MALRFDKNKNEKKRINVKVIATGVVAITLIGIVGISIGKFSSGS
                     PVNLGVASDAITSVGKGINDGFSFIKNGFKDVANFKDNSKKVKKLEEENEKLKKDMIA
                     LNAKLDKTESLEELKKTLNFVQEEYKATSISASVVGKNDGNWYESFVIGAGKNSGVKK
                     ESIVMNGSGLVGIVYEVSNNYSKAISLLDSKASVSFKLAKDANAKGTITQNTTLDNKD
                     SYNSKGYLQGYMFDSSYNVIQGDIITTSGLGFFPDGIPIGEVEKVVDDKDKSLKYVVV
                     KPYVDFKNINDVVVIEPRNIG"
     gene            complement(8185..9195)
                     /locus_tag="PC700_001096"
     CDS             complement(8185..9195)
                     /locus_tag="PC700_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HDF2580416.1"
                     /translation="MTKDMGIDLGTANTLVYIKGQGIVVREPSVVAIRDDSKEVLAVG
                     EEAKKMIGRTPGNIVAIRPMKDGVIADFDITQSMISYFIQKAADKKGVVSPRIAICVP
                     FGVTEVEKRAIEEAARQAGAKDAFLIEEPMAAAIGAGLKVEEPEGNMVVDIGGGTSEI
                     AVISLGGIVTAKSIRIGGDEFDESIVAYVKKEYNLMIGERTAENVKINIGSTFKDDEE
                     INMQIRGRDLISGLPKTIEICSTEVREALKEPVSSIVDAIKSTLERTPPELASDIMEN
                     GIMLTGGGALLRGLDKLITQETGMSVQIAETPLDCVALGTGKSVEDQEIFEKVLMMNT
                     KN"
     gene            complement(9316..9975)
                     /gene="radC"
                     /locus_tag="PC700_001097"
     CDS             complement(9316..9975)
                     /gene="radC"
                     /locus_tag="PC700_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDF2580417.1"
                     /translation="MAPEERPREKMLAKGVKSLSNAELLAILLRTGNKNKNAIELANY
                     IINRDIQGIRHLEDMTIEELCNIDGIGLSKSTQIKAALELGSRVASFKPIKYKIRNPW
                     DIQRYYMDSLRYLKKEVFKAVLLNTKNEIISDVDVSIGTLSSSLVHPREVFKEAIRRS
                     ASKIIVMHNHPSGSVEPSREDKNITSRLIKCGEIIGIEIIDHIIIGDGLYFSFKENMI
                     I"
     gene            complement(10020..10604)
                     /gene="maf"
                     /locus_tag="PC700_001098"
     CDS             complement(10020..10604)
                     /gene="maf"
                     /locus_tag="PC700_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HDF2580418.1"
                     /translation="MNIILASASPRRKEILENTNVRFDIIKNEIDEIILEGETPKHLV
                     MRLAFEKSMSVASEHNEDVVIGADTVVVLDNAILGKPKDESCARDMLKRLSGREHQVI
                     TGISLINLCEDKKVIDYVISNVKFKTLSEQDIEDYLKTNESFDKAGAYGIQGYGALLV
                     EEIRGDYFNIVGLPISRLGDLLKKYFSINLFYGV"
     gene            complement(10824..11795)
                     /locus_tag="PC700_001099"
     CDS             complement(10824..11795)
                     /locus_tag="PC700_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HDF2580419.1"
                     /translation="MVILTAVLVLGIMGLIFGIVLDFASKKFAVEVDERVEAILGVLP
                     GANCGGCGFPGCGGLANAIVEGNAPVNGCPVGGADVGAKVGEIMGISAEAGEKQVAKV
                     ICKGTCSSAKDKYEYEGISDCRAANVLNSGAKMCKFGCLGLGTCKDACKFDAISIVDG
                     IAVIDEEKCVNCGKCKEVCPKGIIITKPESQEVVVECNSKEFGKAVKEKCTAGCIGCG
                     MCVKACKFDAIIFEDKIAKIDPNKCVGCMQCVAKCPTKVISGDITKKKKVTIDQELCV
                     GCTVCKKQCKFDAIEGELKEKHKVDADKCVGCHLCMEKCPKKAIKIL"
     gene            complement(11811..12386)
                     /gene="rsxA"
                     /locus_tag="PC700_001100"
     CDS             complement(11811..12386)
                     /gene="rsxA"
                     /locus_tag="PC700_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HDF2580420.1"
                     /translation="MNLILLFLSIVLVNNVITSQFLGICPFLGVSKKVDTAVGMGVAV
                     TFVLTLASIITYFIQILLVKTGTGFLQTIAFILVIASIVQFVEMVIQKMSPSLYQALG
                     VYLPLITTNCAVLGIALVNVQKSYNLVETIINGFGAGAGFTLAIVIFAGIRERLELAD
                     IPEAFKGFPITLISAGLMSIAFLGFTGLIKL"
     gene            complement(12401..12994)
                     /locus_tag="PC700_001101"
     CDS             complement(12401..12994)
                     /locus_tag="PC700_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HDF2580421.1"
                     /translation="MNLAKVFKNGLIDENPTFVQVIGMCPTLAVTTSAINGIGMGLST
                     AAVLICANLVISLIRKITPDKIRIPIFIVVIATFVTIVGMLLKAYVPALDKALGIYIP
                     LIVVNCLILARAESFAFKTGAMPSIVDGVGQGLGFTVALTIIGAVRELLGNGSLFGMT
                     LFGASFQPVLIFILPPGAFLTLGFLFAGFNKLRSKKA"
     gene            complement(13007..13576)
                     /locus_tag="PC700_001102"
     CDS             complement(13007..13576)
                     /locus_tag="PC700_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HDF2580422.1"
                     /translation="MNSMVRLGGTLLAISAIAALALGATNQVTAPVIEQRNIQENNEL
                     RKAVLPEAKEFKEMDKSTYKDLGDGLIAEVYEGLDGSEVVGYTLKAKPSGYGGEIEVM
                     VGISSEGQVTGVDIGNMSETAGLGAKAKDDAFKGQYKGKTAEPLEVAKGSTTADNQIL
                     AISGATITSTAVTTGVNAAIDVFNSALNK"
     gene            complement(13576..14553)
                     /locus_tag="PC700_001103"
     CDS             complement(13576..14553)
                     /locus_tag="PC700_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HDF2580423.1"
                     /translation="MENKLIVSSSPHVRSNEDTSYIMKQVIIALLPAAVAGVYFFRLN
                     ALSAMFFCILGTVGTEFLYQKFTKQKSTIGDFSAVVTGLLLAFNVPASLPWWMCLVGG
                     IFAILVVKMVFGGIGCNFVNPALAARAFLLASFPVAMTAWTQPGVNWIGKNLDAVTTA
                     TPLSFLKNGAAGLADLSSNGISLADMMIGNIGGCIGETSAILLLLGGVYLMYKGIINY
                     VIPVFYIATVFILTFLLGGFNITFAIYQLFAGGLMLGGFFMLTDYTTSPMTKKGQIIY
                     AVLAGLITTVIRMYGGYPEGVSYSILLVNCLAPLIDKFVRNRVFGEVAK"
     gene            complement(14576..15913)
                     /gene="rsxC"
                     /locus_tag="PC700_001104"
     CDS             complement(14576..15913)
                     /gene="rsxC"
                     /locus_tag="PC700_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HDF2580424.1"
                     /translation="MKLLTFKGGIHPPYRKEYSNTKALEKAQAPKIVYIPLQQHIGAP
                     AKPIVEVGDEVKFGQKIGEQQGFVSCNVHSSVSGKVIAIEQHEVPGGSAQCVVIENDF
                     KEELHESVQPKGQLEDLSKEDIVGIIKEAGIVGMGGATFPNHVKVSPPPDSKAEVVIL
                     NGAECEPYLTADHRLMVENPEDVVFGLRALMKVLDVKKGFIGIETNKPDAIEAIQNVA
                     KDYSEIEVVGLQVKYPQGAEKQLIYACTGKEVPSGGLPIAAGAVVDNVATAAQIAKSI
                     KTGMPLVERITTITGSCIKEPKNLITKVGTLVSEIIEQCGGFKEDKKIGKVIMGGPMM
                     GIAQYTTEIATNKGSSGILCLDEEESRTPDIQNCLRCGRCTDVCPSFLQPLFISAYSL
                     KDDYDTAEYHRAMDCIECGSCSFICPARRPLLQSIRSAKREIGAKRRKQAAQK"
     gene            complement(16129..17073)
                     /locus_tag="PC700_001105"
     CDS             complement(16129..17073)
                     /locus_tag="PC700_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580425.1"
                     /translation="MNRRKTIYRGFNNNRHKIDLRKYVITIACLCLIGYYSYTKIKDS
                     KILEYVSAKIPFLNNSSDITYKDISDELNSIKKGKKSKSQTNSDDKQETNPEKAVNNT
                     KEPEEVKLATIEGWDMYTIQVAAIDNNDDLKKIQTSLVNNDIPFSVMEKDGVKKIQTY
                     SSFDENDVRKQISSVRKVFPDAFLSHLDAPMLSLEYTSNYAYIESISKELNKLITNFK
                     EESSFWSNAENNVDMEKYNTILTNRKAISQNISKEAEKIDYSEMRLFKDNLIEYVKNV
                     NEKIDTASKAANEEKYSVSKSLLLSSMQEYSMFINSIK"
     gene            complement(17184..18458)
                     /locus_tag="PC700_001106"
     CDS             complement(17184..18458)
                     /locus_tag="PC700_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDF2580426.1"
                     /translation="MKKAIAALGIGAVAVSVSSINASALEKGTVTASALNIRSGPSSD
                     CDKVAKLYKGKTVEILEKSNGWYKVRVSSSVVGWGSAKYISTSGSSEGTSNQNNPTSS
                     GTTISGNGKVNVSSRLNVRSGAGTNYSLVGKANNGDVVKLLEQSNGWYKIKLSNGVTG
                     WASSQYISKTSEDVGTNNSSNSNSTNNSDKKPSSEESIEGKNGKVTSAVSLNVRSGPG
                     TSYSIIGKLNGGDVVELKSKNNGWYKVKLSSGTIGWVSASYISETNEGTKEKPNSSSN
                     QNSQSNSNSKPSFTGNSDKSTAKGSTIVDFAYTLIGIPYQWGASGPDKFDCSGFTQYV
                     FKHSVGVSIPRVSREQANFGSAISMGNYAPGDLVYFDTDGDGTTNHVGIYVGNSKFIH
                     CSGTQTNPNKVKVDNLTSSYWSKVLLGARRFV"
     gene            complement(18590..18961)
                     /locus_tag="PC700_001107"
     CDS             complement(18590..18961)
                     /locus_tag="PC700_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2580427.1"
                     /translation="MKFNFCHNNFNVTNLEKSLDFYKKALGLKEVKRKEAEDGSFILV
                     YLGDGITSHTLELTWLRDWDRPYNLGDNEFHLALEVEEFDEAKKLHKDLDCICFENES
                     MGIYFIADPDNYWIEILPKNH"
     gene            complement(19189..19788)
                     /locus_tag="PC700_001108"
     CDS             complement(19189..19788)
                     /locus_tag="PC700_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580428.1"
                     /translation="MESRGYKEDLKKMILDMAHKELESYVSQKGAKKYFEGNLDKIIN
                     NNIKKFNQSIGLNRVYLKMLKKCAYQESVTSISRTIKKEALFKSKKELFAFSEYLKLD
                     INKRLSYNQILRKISRYIYYNRSSYAQKYVVFKRGDEEYLLEPEKIKDELISSYRSKT
                     REDMRSIAKLLDIEVEDDYNAEDIRKKVINYIIKEKLKS"
     gene            complement(19989..20201)
                     /locus_tag="PC700_001109"
     CDS             complement(19989..20201)
                     /locus_tag="PC700_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HDF2580429.1"
                     /translation="MKKKLLVEGMSCGHCVNHLKTALTEDIDGIEVLDVDLENKCASV
                     DMKDDISIEKLKEVIAELGFELKGIE"
     gene            complement(20274..21989)
                     /locus_tag="PC700_001110"
     CDS             complement(20274..21989)
                     /locus_tag="PC700_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2580430.1"
                     /translation="MKRIKVLTVVLAITLMVAGCSNTKTKQSSSDSSLQSQSSIDAKY
                     INLTMVTPKTINPITNTDKSVGYIMNLVYDSLFTIDENYNVIPQLVKEYNIAQDGMSI
                     DIKLKDAKWHDGKNVTSNDVNYTIGLIQKSADSPYNEFTKNIASVNIKSDKDFTIKFK
                     ARYAFSIDSLIFPIVSQNHLDSKDVNDKNNNMIGNGKYKIESYTEREGMVLSVNKDYY
                     EEVPKTMKNIKVGMVPNEEARTSMVMALDSDITNVTLNDLSKFQEKEFNITKYQGRDY
                     ECVLFNYNNPFFKDVNFRKAIAHSIDKDRIISEGYMDDATPVNFPLNSKSKYYNSEMK
                     DLEFNKDKAIECLAKVEYANINSVNQNDNKVKNQKKSAKKNSKKLTPEEEAKAKKAEE
                     DRIKKEAEKKKNREKEEVKKSLSEMNLKIIVNRDNSERIKTANIINENLKAIGINSTV
                     NQLSDKDMENALNSKNYDLALVGWKLSIIPDASSIIASSGYTDDKLNGYMSALTSSTS
                     EIETKKAYKDVQTYIKDNATFISLAVRNNYIVSNSRLKGKITPNDFDVYEGISNLDIK
                     SNESN"
     gene            complement(21989..22543)
                     /locus_tag="PC700_001111"
     CDS             complement(21989..22543)
                     /locus_tag="PC700_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HDF2580431.1"
                     /translation="MNSKKVLILSIFIILFGALLMESKVIHKFLYPKKYSEYVEKYSK
                     EFNLDENIVYSVIKAESKFNSSAVSKKEAKGLMQILDITRDWGAEELNLKNVDIFDPE
                     TNIRLGCWYLSKLYKEFGKLDLVIAAYNGGSGNVKKWLENNEYSKDGENLHDIPFKQT
                     SKYVEKVKNNYEHYNKIYGKKGKN"
     gene            complement(22536..23138)
                     /locus_tag="PC700_001112"
     CDS             complement(22536..23138)
                     /locus_tag="PC700_001112"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDF2580432.1"
                     /translation="MLILGLTGGIGCGKSSLSNIFRNLNISIVDADIISRKIFEDKLL
                     LEKVFVHFGQSIKNDDGTLNRKALGKIVFSDEEKLKELNSLTHPRIREKIISEIEELR
                     KKGENIVVLDAAILVESGFLDMVDKLLVVTCKQEVQISRIQKRDNCSKQEALSRINSQ
                     MSQEEKSKYGDYIIDNSGTITELESKAHKFIEYMKENWRE"
     gene            complement(23147..25795)
                     /gene="polA"
                     /locus_tag="PC700_001113"
     CDS             complement(23147..25795)
                     /gene="polA"
                     /locus_tag="PC700_001113"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDF2580433.1"
                     /translation="MEKKLIIIDGNSIINRAFYALPEMNNKEGLKTNAIYGFTTMLFK
                     MIDIYKPTHISVAFDRKAPTFRHLEYKEYKAGRKGMPDELAEQLQPLKDLLDKFKINR
                     LEIDGYEADDIIGTVSKKAENNGYKVYIVTGDKDAIQLASDNTTTLITKKGVGEVEEY
                     NFNSVIEKYEMTPAQFIDLKGLMGDKSDNIPGVPGIGEKTGIKLIKEFSSIENLIENT
                     DKLKGSVKKKIEENKEIAIQSKRLATIIRDVPIEVDLDSMVFGDYDKDELLDKFRYFG
                     FTSLLSRLVDLVDSDDTEHVEEKIEILKLKDTEKFIDEVNKKGQVILKTVRGQGNILE
                     KNIIYIFISVDGEKIYYVDSNEVSKLDSIFSNNEIKKYGYNLKDDYISLKPYNINLEN
                     MYFDITIAEYLIDSTSSTSYDCSAIAMKYLSKKVKSKEELLGKGVKSKNYEDLEFEDL
                     AEYMGQIICTVKETIPMMERSLMDMSMDGLFYHVEMPLVEVLGHMEYEGIKVDKSILD
                     ELSVEFKEIIATLEKEIYELSGEPFNINSPKQLGVILFEKLELPIIKKTKTGYSTNAD
                     VLEKLRDKHPIIDKITEYRQIVKLNSTYVEGLLNIINPESNRIHSSFNQTITTTGRIS
                     STEPNLQNIPIKMEMGRKIRKVFIADDNCKLVDADYSQVELRVLAHMSQDENMIEAFK
                     HHEDIHTKTASQVFNVPMEDVTSELRGAAKAVNFGIIYGKSDFGLADDLNIPVPKAKE
                     YIESYFAKYHKIKEFMDTTVEKASEDGYAVTILNRRRYIPEIKSSNFMERNRGKRFAM
                     NAPIQGSAADIIKIAMINVHKKLEENNLKSKLILQVHDELIVEAIDDEIDIVKKIVKE
                     EMENAVNMDVHLDVDLNVGSSWYETK"
     gene            26114..26196
                     /locus_tag="PC700_001114"
     tRNA            26114..26196
                     /locus_tag="PC700_001114"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26148..26150,aa:Leu,seq:caa)
BASE COUNT         7996 a         5161 c         2939 g        10523 t
ORIGIN      
        1 gacttaattc tatttcatat tattaaaata gagtaatagt ttaaattcta ctactctatc
       61 tataatctat ttcctaatta tttaatctca aatttagctc ctcatcattt aaatctttca
      121 taagatttct acaggtacta ggtttattgc aacagaaaaa ctagggcatt aaacacttat
      181 atatctgttt ctttagactg taatattaat attttttaaa tacgttgact tttgcttttt
      241 ctaacttctc tccatgatag ttctcctcta tcaagagctt ttataaatat ttctgctgaa
      301 gctagatttg tagctattgg aacatgataa acatcacaaa gtcttattaa cgcttgtata
      361 tctgtttcat gagcctgaga tgtcaatggg tctcttaaaa aaataactaa atctatttct
      421 tgtgttacta ttaaagaacc tatttgttgg tctccaccta aaggaccaga tgctaatcta
      481 tttatattta attcagtttc atccattatt ctttttcctg tagttccagt tgcatatagt
      541 ccatactttt ttaaaattga ttcataacct atacaaaatc cgaccatagt gtttttcatt
      601 tggtcatgtg ctactaatgc tatattcata tatctaatct cctcaattta tgtttagatg
      661 tatttaaatt tagaaattta ttttctttct tctcatacac acatttagta ctaatcctaa
      721 gtttgctaaa gatgtcaata acgaacttcc tccataactt acaaaaggta atgttactcc
      781 agttactggt attaacgcaa ccgtcatacc tatattttgg attatctggt aaccaaacat
      841 tcccataaca ccaaccacta tgagggttcc ataaaaatct tttgcatctc ttgcaattgc
      901 taaaagcctt aataggaata tcataaataa gattatcaac actgccattc ctattacacc
      961 aagttcttca cctacaacag caaatataaa atcactatct tgtacaggta aaaaatcttc
     1021 ttgattttga cttccattgt aaagtccttt accagttacg cccccagaac caattgcaat
     1081 cagagattgc ataacttgat agtttccttt tagagttaca tcttctggat ttagaaaagc
     1141 ttctattctt accttttgat ggtctgccat cattaaatac atcagtggca ttgacactaa
     1201 tagtatgatt attcctcttt ttatcaactt tgagctaaga cctgctgtga acagcattgc
     1261 aaatatcata caacaaaata caattcctgt tcccaagtct ggttgtgcaa tcaataatcc
     1321 gataaatgga agagaataaa ctacaacagg cataacttcc tttaacgtat ttaatttatc
     1381 ctttttggat tcaagtatct ttgcataact tagtacaaat gttaatttga ctatttcaga
     1441 tgtctgcaag tcaagtggtc ctaagttaat ccacgaccta gctccacctt ttactgtccc
     1501 aattccaggt aataaaacta tggctaacaa aataagactt attatataaa gcgctttata
     1561 atacctgcct agaagattat agtcaaaaaa taatatgact ataatcattc ctataccaag
     1621 tacaaaagct aatccttgtt taatcaattg attatatgaa cctgttgaat tagcatgtgt
     1681 tgcactacta agtataacaa gcccaaaaat aaagatagct aatactgtaa ctataagttt
     1741 ccagtctaac tgttttatca acttaattgt cgacttataa ttaatattga tttttttcaa
     1801 agaatcaccg tctttctatc tttaaacata taattaaata aaaggggcat ataccaataa
     1861 ggatatcccc cttttaagca ctaattacat acatctatct tttatattct ttagtggtat
     1921 attagcaact aatgcagata caggtttgtc atcatgctca cctctgcatt tagacatttt
     1981 tatttcaaga ccatcatctt ctatttctgc ataattagct attactttta aaatgtctgt
     2041 ttttatcatc tctaaaagtt gtggtgaaca atctactcta tcatgaacta aaactagctt
     2101 taacctctct ttagcaacag atttactagt tttagcctcg ttagaaaaaa ctctaaataa
     2161 atctaacaca gatgtatcct cctttgctac ttagccatgc taaacatttt tttaagtcta
     2221 ccaaagaatc catcttcaac ttcaagatca agtagaggaa cttcttcatt tagtatcctt
     2281 tttgcgatat ttttgtatgc ttgaccagca agtgacttag aatcaagtat agctggctct
     2341 cctttatttg ttgatataat tatgctttca tcatcaggaa caagacctaa caaatctatt
     2401 gctaatattt ctattatatc ttgtttatcc atcatgtctc cacgcttaac catatcattt
     2461 ctaattctat ttataactaa tcttatctct tttatttcat ttgcttctaa tagacctatt
     2521 attctatcag catctcttac tgctgatatt tctggattag taactacaat agctctatct
     2581 gccccagcta ctgcattttt aaagccttgc tcaatacctg ctggacagtc tattattata
     2641 tactcgaaag attcttttag tttctcacac agatcaatca tctgctcaac tgagacagca
     2701 tttttatctc tagtttgtgc tgctggtaat aaatatagat tatcaaatct tttgtctttt
     2761 attagagctt gcttaagcct acaagttcct tcaactacat ctactatgtc ataaactatt
     2821 cgattttcaa gacccattac tacatctaag tttctaagtc caatatctgc atccacaact
     2881 actgtttttt tattttctag gcttagcgca gttcctaagt ttgctgcggt agtagttttt
     2941 ccaaccccac ctttaccaga tgttataact ataacttcgc tcataatact accccctatt
     3001 tttctttcta tttgtctaac tttgacaaat agctttcgat tacaattctt ccattactta
     3061 caaaggctat ttctgggcta atttctgatt cattttcata attttcttca tcatctggag
     3121 cttctgcaat attatctgct atttgtaata ttttaggatt aagatttcca gcaacaacat
     3181 atgcaaattc attgccttct gcacctgcat gtacaaaacc ttctatttta cccattacaa
     3241 aaacattacc tcctgctaca accttagcac cagcatttat atctcccata acaactgtat
     3301 ttgattttga aaggactttt gaaccaggtt tcatatcatt caatataact acatcacctt
     3361 caaattcaat attttcacca gatcttaggg tattgacata ttttgttgga aaattactat
     3421 tttcttcaat tttttgatct tcaacaaatt ccacatcaaa tctagaagta attccttctt
     3481 taagttctag aatctccatg tcagttaaac aatcactatt aattttacta attttagccc
     3541 cattaaaaaa gccaacatat gcttccaatt tgtttattat tttttcctgt atttcctcaa
     3601 atggagcttc tctttttata tttactatta tccctctttt attacccttg aactctacta
     3661 gttcttgact acaaatttct cttaaagaca taacaacctc cagagtgata tatcatttat
     3721 attcttcaaa tacgctttaa acccttttat tttttctata taaagctaaa gtttatacat
     3781 agattaagct atctattaat ttgtgactcg aaatttatat tttcgtcaga agaattgtta
     3841 acatcattcg tcttgttgtc atttttacta ttagttggct ctaatcccat ataagttcca
     3901 ataacttctc tagttggtaa aagtgagaat acacttgaat caccttgagg aattacacat
     3961 acaacagcta tttctgggtc atctgctgga gcaaaagcaa cagtccatgt aaaagaacca
     4021 taatcttgtt taaatctatc tatttggtca tctgttattt ttggatttaa ttcttttata
     4081 gcttttctta gatatgaaga attcacctta tcagtgtcct ctaatctaac ttttactcca
     4141 tcttctaatt cttcctctaa tttctttctt tcttcgtctt tcaaatcttt attttctaat
     4201 ttctttttta tttccttttc cttagctaat gaaagctctt tttctttttc tgctttcatc
     4261 ttatcagcta gtttaatcgc atcatttagg ttgacattat aagatgacat atgacttttt
     4321 aaatattcat actcgttatc tgtaggtatt ttcccacttt tctcagctgt accagttttt
     4381 gctgctacat ctattggaaa atcagcaaaa gcactcttcg ctgtaccttg tcttgcaacc
     4441 aatttcatac cttttgttaa ttcttttaag ttatctggat ttttaaaagg tattttttcg
     4501 actttcttct ggtcattaat tttaactgat gaataatcac ttgaaacagc tctatcaact
     4561 acagataatt ctactaaatt acctccattt gctatagcag ctacatatct tgatatttga
     4621 gctggtgtat aagcattttc accttgacct atagctaagt taaatgtatc tgcagtactc
     4681 cattttgcaa agttaaaata actgaatact gctaaatctg ctacttcttc tattctatct
     4741 tctttaacat tcattttctt tagcctattc attgtttcga ctctaccagg agttttcttt
     4801 tctgctgccc agcttacaat ctcattgatt cttttctcat attctttagg atttttttct
     4861 ctagttatat caacaaaatc atttgccatt tctctttcta aatttgactt tagaagagct
     4921 tgtgttgatt tcaatttatc ttctgtgcta ggaactttac ctgctctttc ttctacttca
     4981 tcttgaagcc ctgtattttg gtcaagccca aataattttg catactcaag tattttattt
     5041 gcattcatat ctataccaat gctttttcca tctggccatg ttttacctgt accaattgtt
     5101 gccatatata tattacaaga ctcttgtatc gctttatata agtttgtcat accatggttt
     5161 cctctaccat ggttccatac atagtcacca aaagattttt tacctaacat tataacccca
     5221 gggtcattta tagtaaagtt tggatccaaa ccattttcta atgctgccat tgaagttagc
     5281 atcttaaatg aagaacctgg ctgaaattcc ccttgtgtaa ccaagtttaa aagtggactt
     5341 cctgctagta agtcatttgg attttttggt tgaagtgctt tataatcctc tgtagaaata
     5401 ccttttgcaa atttatttgg gtcataatta ggataacttg atgaagctaa tacttctcca
     5461 tttttaacat caatagctat aagagctgct gactgtgctt ttcctgcata tgcactaatt
     5521 ggtttgtccc caaatttact tttaaaagta cctcctttag aagcaacttc gattatctgt
     5581 tttaatgcat tatctgaaac ttcttgtaaa tctttatcta gtgtaagata cacagtatct
     5641 cctgatttag gttttttaga ttcaatttct ttactgattc tacccaatgc atcaacttta
     5701 accattttat aaccatcagt tcctcttaat ctactttcat tactcttttc tactcctgca
     5761 agacctacca tatctccagt ttcataacct ttttgaaggt atgattctat ttgagtggat
     5821 ggcatttttc ctacatatcc taacatatga gaagctaatg acccattagg ataatatctt
     5881 actggttcta ctgctattga aacaccaaca aggtccatag cactttcttc aatttgagca
     5941 atcgtagttt ctcctacatc cttagcaata gttactggat tatattgaga atatcctttt
     6001 gattttatca aatcccttac gaccattatt tttcttgcat cttcatcact caaactttta
     6061 tcaatttcta atgcatcact atttcttact ttttcaaacg cttctttggc actcagttta
     6121 gtttctttta tcttataact agccaaccaa tctctcttat ctctttctgc tgtgaacatc
     6181 catttactaa ctaaaatagg tggataatat ccattttcat ttagttttgc ctgtaatctt
     6241 gtcgcatcca atcttttatc ttcttgagat aatattccag ctgatataag tttgtctact
     6301 aagtaataaa aactttcctt agcattataa tcatttggaa taccattttc tgatttatat
     6361 tccctaatat ctctgtcata tgtataatag tactttccat tttctacata tataggaaac
     6421 tcatctacat attcttcacc atttctctcc agcaaattta taagttttaa tgatatttca
     6481 tttgctcttg aatgcttatt tgcatctttc ttattaatat cgttacctga aacttgaaca
     6541 gtaaataagt ttttatttcc tgccaacagt cttccatatc tatcttttat ttctcctctt
     6601 ggagcttcta ttgccaactt tttatatgtt ttattctcag ctaactcatt gtaatattca
     6661 tatttaaatg tagtcatata gattattttt actaaaatag ctaaaaatat cacgctcatg
     6721 actatcttaa ttatttttag tctatcaact tgtttatttt cttccatgaa atcaccaact
     6781 aatcttcttt aagttttaaa actgctcttt tagataagcg atatattact aaaccaatta
     6841 aactattcac tattggtgct actagtagac cttttacaaa aagtgttgat atgttgtagc
     6901 tttgataaat tatactagat gtacctatat tgacaattga atcaattaaa ctgcctataa
     6961 aaattaaaac gaaagtaata atataactac ctttatatag ctgatctctc atgtatccaa
     7021 ttgcatatgc aataataaac aaaattaatg catttacacc aaaaattcca ccaacggtta
     7081 tgtctttaat aagccctatt attgctgcta taatcccacc ttctaattca tctaaataaa
     7141 gagagattat ggttacatat ataagaacta aattaaagct tatgccaaaa atatctatat
     7201 aatttgttat actattttct atagtcataa gcacaatccc caacagacaa agtaaaactt
     7261 ttttcattgt ttttatcccc tttatcctat atttcttggt tctatgacta caacgtcatt
     7321 tatattctta aaatcaacat aaggttttac aactacatat tttaatgact tatctttatc
     7381 atcaaccacc ttttcaactt cgccaattgg tataccatct gggaagaatc caagacctga
     7441 tgttgttatt atatctcctt gaattacatt atatgatgag tcaaacatat atccttgcaa
     7501 atatccttta ctattataac tatctttatt atctaaagta gtattttgag taattgtacc
     7561 tttagcattg gcatcctttg ctagtttaaa gcttacagat gccttagaat ccaatagtga
     7621 aattgcctta ctataattgt tggaaacttc ataaactatg ccaactaacc cactaccatt
     7681 cataactata ctttcttttt ttacacctga attttttcct gcacctatga caaaactctc
     7741 ataccagttt ccatcatttt taccaacaac acttgctgaa atacttgtcg ccttatactc
     7801 ttcttgtaca aagttcagtg tcttttttaa ctcttctagc gattctgttt tatctagttt
     7861 ggcatttaaa gctatcatat cttttttaag tttttcattt tcttcttcca atttttttac
     7921 ctttttagaa ttatctttaa aatttgctac atctttaaaa ccatttttga taaaagaaaa
     7981 tccatcattt attcccttac ctacacttgt aatagcatct gaagctactc ctaaatttac
     8041 tggactacca ctagaaaatt taccaattga tatacccaca attccaatta aagtgatagc
     8101 aactacgccc gttgctatca ctttaacatt aatccttttt ttctcatttt tgttcttgtc
     8161 aaatctcaag gccatcacca acttctaatt cttagtattc atcattaata ctttttcaaa
     8221 gatttcttgg tcttcaacag atttacctgt tccaagtgca acacaatcaa gaggtgtttc
     8281 tgctatttgt acagacatac cagtttcctg tgtaataagc ttatcaagac ctctaagcaa
     8341 agcgcctcct cctgttagca ttattccgtt ttccattata tctgaggcta attctggtgg
     8401 agttctttct agtgtagatt ttattgcatc tactattgaa cttacaggtt cttttaatgc
     8461 ttctctaact tctgtagagc atatttctat tgttttaggt aatcctgaga ttaaatctct
     8521 acctcttatt tgcatattta tttcttcatc atctttaaat gtagaaccta tgttaatttt
     8581 tacattttca gcagttcttt cacctatcat aagattatat tcttttttta cataagccac
     8641 tatagactcg tcaaattcgt ctccacctat tcttatagat ttagcagtaa cgattcctcc
     8701 taaggatatt acagctattt ctgaagtacc tccaccaata tcaactacca tattaccttc
     8761 tggttcttca acttttaatc cagcccctat agccgctgcc attggctctt ctataaggaa
     8821 tgcatcttta gctccagctt gtcttgcagc ctcttctata gctctctttt ctacttctgt
     8881 aactccaaaa ggaacacata tagctattct tggacttaca acaccttttt tatcagccgc
     8941 tttttgtata aaatagctaa tcattgattg agttatgtca aaatcagcta ttactccatc
     9001 tttcataggt cttatagcta ctatatttcc tggtgttcta cctatcattt ttttggcttc
     9061 ttcacctact gctaatacct ctttactatc atctcttatt gcgacaactg atggttctct
     9121 aacaacaata ccttgtcctt ttatgtaaac taatgtattt gcagtcccta aatctattcc
     9181 catatccttt gtcatcttat taaaactaaa aaaacctttt ttttctttct ttttttcctt
     9241 agccatgatt ttactccttc ctactattaa gaatcatgtt taaaacattc tcctattatt
     9301 ttatcacata taaagtcaaa ttatcatatt ttctttaaag ctaaagtata atccatctcc
     9361 aataattata tggtctatta tttctattcc aattatttct ccacatttaa taagtcttga
     9421 tgtgatattt ttatcttctc ttgatggttc tacacttcct gaggggtgat tatgcatcac
     9481 tattatctta cttgcacttc ttcttatagc ttctttaaaa acctctcttg gatggactaa
     9541 tgacgagctt aaagtcccta tagatacatc tacatcagat attatctcat tttttgtgtt
     9601 taaaagaact gctttaaaaa cctctttttt taaatacctt aggctatcca tataatacct
     9661 ctgtatatcc caaggattcc ttattttata ctttataggt ttaaaactag ctactctaga
     9721 gcctagttct aaagctgctt taatttgggt tgattttgat aacccaattc catctatatt
     9781 acacagctcc tctattgtca tatcttctaa atgtctaata ccctgaatat ctctatttat
     9841 tatatagttg gctaattcaa tagcattttt attcttgttg ccagttctta aaagtatagc
     9901 caacaactca gcatttgata agctttttac gcctttggca agcatctttt ctctgggtct
     9961 ttcctctggt gccatttctt ttaccttaat ggttgaatta aatgactttt tcagataaat
    10021 cacactccat aaaaaagatt tatactaaaa tattttttta ataggtcgcc taaccttgaa
    10081 attggaagac caactatatt aaaataatct cctctaattt cttcaactaa caaagctcca
    10141 taaccctgta taccataggc tcctgctttg tcaaatgact cattagtttt cagataatcc
    10201 tcaatatcct gctctgataa cgttttaaat ttcacattgc tgattacata gtcaattacc
    10261 tttttatctt cgcacaagtt tattaagcta atacctgtta tgacttgatg ctctcttcca
    10321 gatagtcttt ttaacatatc tcttgcacaa gactcatctt ttggttttcc taaaatagca
    10381 ttgtctaaaa caacaactgt atcagctcca ataacaacat cttcattatg ttcagatgct
    10441 acagacatac ttttttcaaa agctaatctc ataactaaat gttttggtgt ttcaccttcc
    10501 aatataatct catcaatttc atttttaatt atatcaaatc ttacattcgt attctctaga
    10561 atttctttcc ttcttggaga ggctgatgcc aagattatat tcatttattc acctcatttg
    10621 tacgtaattt gtacctattt agtctatcac tatattgtaa aaaattcaac agaaatgtcg
    10681 aacctttttt gttacactat tgttatattt attaccatta gaataaatat acataaaata
    10741 gaggtatgga aattattcca tacctctgtt ttatgtactc attagagata tggaaattat
    10801 tccatatcct tattttgtcc atattacaaa attttaatag ctttctttgg acatttttcc
    10861 atacataaat ggcatcctac acatttatct gcatctacct tatgtttttc ttttaattct
    10921 ccctctattg catcaaactt acattgtttt ttgcacactg tacatccaac acaaagttct
    10981 tggtcaatag taactttctt tttcttagtt atgtcacctg atattacttt tgtagggcat
    11041 tttgctacac attgcataca ccctacacac ttattagggt ctatcttagc tattttgtct
    11101 tcaaatatta ttgcatcaaa tttacaagct tttacacaca ttccacagcc aatacatcca
    11161 gcagtacatt tttccttaac agctttacca aactctttgc tattacactc tacaactact
    11221 tcttgacttt ctggttttgt gattattata cccttaggac aaacttcttt acattttcca
    11281 caatttacac acttttcttc atctattact gctattccat caactataga aatagcatca
    11341 aatttacaag catccttaca tgttccaaga cctaaacatc caaatttaca catcttagca
    11401 cctgagttta aaacatttgc tgctctacaa tctgatattc cttcatactc atatttatct
    11461 tttgcacttg aacaagttcc tttacaaata acttttgcta cttgtttctc acctgcttca
    11521 gcacttattc ccattatttc tccaactttt gcaccaacat ctgctccacc tacagggcaa
    11581 ccattaactg gtgcattgcc ttcaactatc gcatttgcaa gacctccaca acctggaaat
    11641 ccacaacctc cacagttggc tcctggaagt actcctagta tagcttccac tctctcgtct
    11701 acttctacag caaacttttt agatgcgaaa tctagtacta tcccgaagat taagcccata
    11761 attcctaaaa ctaatacagc tgtaagtatc accattttca cccctctttt ttatagcttt
    11821 ataagtcctg taaatcctaa gaaagctatt gacattaaac ctgctgatat aagcgttata
    11881 gggaaacctt taaatgcttc tggtatatca gctaactcta gtctttctct tatacctgcg
    11941 aatattacta ttgctaaagt aaatccagct ccggctccaa atccatttat tattgtttct
    12001 actaaattat aacttttttg aacattaact agagctatac caagaactgc acagtttgta
    12061 gttataagag gtaaataaac tcctaatgct tgatataaag atggactcat cttttgaatt
    12121 accatttcaa cgaactgaac tatagaagct ataaccaata taaaagctat tgtttgtaag
    12181 aatccagttc cagtttttac taataaaatc tgtataaaat aagttataat tgaagctaga
    12241 gttaaaacga acgtaaccgc aactcccatc cctactgctg tatcaacctt cttagaaact
    12301 cccaagaaag gacatatacc caagaattga gatgttataa cgttattaac aagaacgata
    12361 cttaaaaata aaagtattaa attcatttat tacacctcca ctatgctttt ttacttctta
    12421 atttattaaa tcctgcaaat aagaatccta atgttaagaa tgcacctggt ggtaatataa
    12481 atattaatac tggttggaaa gatgctccaa ataatgtcat tccaaataaa ctaccatttc
    12541 caagtaattc tcttactgca cctattattg taagtgcaac agtaaatcca agaccttgac
    12601 ctacaccatc aactattgaa ggcatagctc ctgttttgaa agcaaaactt tctgcacgag
    12661 ctaatattaa acagttaaca actattaatg gtatatatat accaagagcc ttatcaagag
    12721 caggaacata tgcttttaaa agcataccta ctattgtaac aaatgtagct ataactacta
    12781 taaatatagg tattcttatc ttatctggtg taatttttct tattaaagat ataactaaat
    12841 ttgcacatat taaaactgct gctgttgaaa gacccatacc aattccattt attgctgaag
    12901 ttgtaacggc tagtgttgga cacataccta taacttgaac aaatgttggg ttctcatcta
    12961 ttagcccatt tttaaatact ttagctaaat tcataattaa ccctccttat ttattaagag
    13021 cactattgaa aacatctata gcagcattta ctcctgtagt aactgctgta gatgtaatag
    13081 ttgcacctga tattgctaat atttgattat ctgctgttgt acttcctttt gctacttcaa
    13141 gaggctcagc agtttttcct ttgtattgac ccttaaatgc atcatcctta gcttttgctc
    13201 caagacctgc tgtttctgac atattaccaa tgtcaacacc tgtaacttga ccttcagatg
    13261 atatacctac cataacttca atttctccac catatccact tggtttagct tttaaagtat
    13321 atccaaccac ttcagaacca tctaaacctt catacacttc agctataaga ccatctccaa
    13381 ggtctttata tgtactttta tccatttctt taaattcttt tgcttctgga aggactgctt
    13441 ttctaagttc attattttct tgtatatttc tttgttctat tactggagct gtaacctgat
    13501 tagtagctcc aagagctaat gcagctattg cacttatagc aagtaaagta ccacctaatc
    13561 tcaccatact gttcattatt tagccacctc cccaaatact ctatttctaa caaacttatc
    13621 tataagtgga gcaagacagt tcactaatag tattgagtaa gatacacctt ctggatatcc
    13681 accatacatt cttataacag ttgttataag accagctaat acagcgtata ttatttgacc
    13741 ttttttagtc ataggcgaag ttgtataatc cgtaagcatg aagaatccac ctaacataag
    13801 tcctccagca aacagttgat atattgcgaa agttatatta aatccaccta aaaggaatgt
    13861 taatataaat acagttgcta tgtaaaatac tggtataaca taatttatta tgcctttata
    13921 cattaggtat actccaccta ataataataa tattgctgat gtctcaccta tacatccacc
    13981 aatattccct atcatcatgt cagcaagact aattccattg ctagaaaggt ctgccagtcc
    14041 tgctgctcca tttttcaaaa agcttaatgg agttgctgta gtaactgcat ctaaattttt
    14101 acctatccag ttaacacctg gttgagtcca agcagtcatt gctactggga atgatgctaa
    14161 taaaaatgct cttgcagcaa gtgctggatt gacaaagtta catccaattc caccaaatac
    14221 cattttaact actaatattg caaatattcc tcctactaga cacatccacc aaggaagtga
    14281 tgctggtacg ttaaatgcta ataataatcc tgttacaaca gctgaaaaat ctcctatagt
    14341 acttttttgc ttcgtaaatt tttgatataa aaattcagta cctactgtac caagtataca
    14401 aaaaaacata gcactcaaag cattaagtct aaagaagtat actcctgcta ctgctgctgg
    14461 aaggagtgct ataataactt gtttcattat atatgaagta tcttcattac ttctcacatg
    14521 aggagaagat gatactatca acttattttc catcgttttt cccccttaac tttctttatt
    14581 tctgagcagc ttgctttctt ctctttgctc ctatttctct ctttgctgat cttatagatt
    14641 gaagtaatgg tcttcttgct ggacaaataa atgaacaaga cccacattct atacaatcca
    14701 ttgctctatg atattcagct gtgtcatagt catcttttaa agaatatgca cttatgaaaa
    14761 gtggttgtaa gaatgatgga catacatctg tacatcttcc acatcttaaa cagttttgaa
    14821 tatctggagt acgtgattct tcctcatcca agcataaaat tcctgaagaa cctttgttag
    14881 tcgctatttc agtagtatat tgagctattc ccatcatagg tccacccatt ataaccttac
    14941 caatcttctt atcttcttta aatccaccac attgttctat tatttctgaa actaatgttc
    15001 caacttttgt tattaggttc tttggctctt tgatgcaact acctgttatt gtagtgattc
    15061 tttcaaccaa aggcatacct gttttaatag acttagctat ttgtgctgct gtggcaacat
    15121 tgtcaacaac tgcacctgct gctattggta atccaccaga tggaacttct ttgcctgtac
    15181 acgcataaat aagttgtttc tctgctcctt gtggatattt cacttgaagg ccaacaactt
    15241 cgatttcact atagtcttta gctacatttt gaattgcttc tatagcatct ggtttattcg
    15301 tttcaattcc tataaatcct tttttgacat ctaatacctt cattaatgct cttaaaccaa
    15361 acacaacatc ttctggattt tcaaccatta atctgtggtc tgcagttaag tatggttcac
    15421 attctgcacc atttaatata actacttctg ccttgctatc tggtggagga gatactttta
    15481 catggttagg gaatgtagct ccacccatac ctactattcc tgcctctttt atgataccta
    15541 ctatatcttc tttgctcaaa tcctctaact gaccttttgg ttgaacactc tcatgtaatt
    15601 cttccttaaa gtcattttcg ataactacac attgagctga acctcctggt acttcatgtt
    15661 gttcaatagc aataacttta cctgatactg aagaatgaac gttacatgaa acaaaacctt
    15721 gttgttcacc tattttttgc ccaaacttta cttcatcacc aacttcaaca ataggtttag
    15781 ctggtgcccc tatatgttgc tgaagaggaa tgtaaactat ctttggagct tgagcttttt
    15841 caagtgcctt tgtgttagag tactcttttc tatatggagg atgtatccct cccttaaaag
    15901 ttaagagttt cattgtttta cccccttatc tataaaaaaa cattttttct gccttctata
    15961 agtatactat atatattaaa aaatcttaac aattgttaaa aaaatatccg tatttttata
    16021 atttatttta taaatcaata cctctatatt aagtcaaact gaggaaaagg attgcttaat
    16081 taaacaatcc ttttcatata gctttctaaa gtccaaattt tctgtatttt attttataga
    16141 atttataaac atagagtact cctgcatgga tgataaaagc agacttttac ttacactata
    16201 tttttcttca tttgctgctt ttgaagctgt atcaattttt tcattcacat tctttacata
    16261 ctctattaaa ttatctttaa ataaacgcat ttcagaatag tcaatctttt cagcttcttt
    16321 tgatatattt tgtgatattg cctttctatt tgtcaaaata gtattatatt tttccatatc
    16381 tacattattt tcagcattcg accaaaaact tgattcttct ttaaagtttg ttattaattt
    16441 atttaattct ttagatatac tctctatata agcgtagtta cttgtatact ctaaagacaa
    16501 cataggtgca tctaagtgtg ataaaaatgc atctggaaac acctttctta cactgcttat
    16561 ctgctttcta acatcatttt catcaaaaga ggaataggtc tgtatttttt ttactccatc
    16621 cttctccata actgaaaaag gtatgtcatt attaactaat gatgtctgta tttttttcaa
    16681 atcatcatta ttatctattg cagcaacttg aatagtgtac atatcccagc cttctatagt
    16741 agctaatttg acttcttctg gctctttagt gttatttacc gctttttctg gattagtttc
    16801 ttgtttatca tctgaattgg tttgtgattt agattttttc ccttttttta ttgaatttaa
    16861 ttcatcagat atatctttat aagttatatc tgatgaatta tttagaaatg gtattttagc
    16921 cgatacatac tccaatatct tgctatcttt tatttttgta taagaataat agccaattag
    16981 acacaagcaa gcaatcgtta taacatattt acgtaaatca atcttatgtc tattattatt
    17041 aaatcctctg tatatcgttt ttcttctatt cattagtttg tccctcccaa aattatatta
    17101 ttaataaagt atatttttaa gagtcacaat ttatgaatat aaataattat ttcttctaat
    17161 tttttaataa gaaattgtac ttactaaaca aatcttcttg caccaagtaa tactttagac
    17221 caatatgaac tagttaaatt atccacttta accttattag gattagtctg agtaccacta
    17281 caatggataa atttactatt tcctacatat atacctacat gattagttgt tccatcacca
    17341 tcagtgtcaa aataaactaa atctcctggt gcataattcc ccatagaaat tgcacttcca
    17401 aaattagcct gttctctaga aactctagga atagaaacgc ctacagaatg tttaaaaaca
    17461 tactgtgtaa atccagaaca gtcaaattta tcaggtcctg aagctcccca ttgatatggt
    17521 attccaataa gagtatatgc aaaatctacg attgttgaac cttttgctgt agatttatct
    17581 gaatttccag taaatgatgg ctttgagtta ctattagatt gagaattttg gttagatgat
    17641 gaatttggtt tttcttttgt accttcatta gtctcagaaa tataactggc actcacccat
    17701 ccaattgttc cactcgataa ctttacctta taccaaccat tatttttgga ttttaattca
    17761 acaacatctc caccatttaa ttttcctatt atagaatagc ttgtcccagg accacttctt
    17821 acatttaaac tcacagcaga tgttacctta ccattcttgc cttcaattga ttcttcgcta
    17881 gatggttttt tatcagaatt attagtacta tttgaattac tagaattatt cgttccaaca
    17941 tcctctgatg ttttagagat atattggcta ctagcccatc cagtaactcc atttgatagt
    18001 tttattttat accatccatt gctttgttct agtaacttta ctacatctcc attatttgct
    18061 ttaccaacta atgaatagtt agtacctgca ccacttctta cattcagtct agaactcaca
    18121 tttactttac catttccact aatagttgtc ccacttgaag ttggattatt ttgatttgat
    18181 gtaccttcag atgaaccact tgttgatatg tattttgcgc ttccccatcc aacaacagaa
    18241 ctcgataccc ttactttata ccatccatta gacttttcta aaatctctac agtttttccc
    18301 ttgtataatt ttgctacctt atcacaatca gaacttggtc cacttcttat attcaaggca
    18361 cttgctgtta ctgttccctt ttcaagagca cttgcattta tggagctaac agatactgct
    18421 actgccccaa ttcctaaagc tgctatagct ttcttcacta ctatcactcc ccccaacaaa
    18481 tttttatagt cataaattca taacattatt atccattttt agtccctaca atttaatgat
    18541 aacttattct cttattaatt gtaaagaaat tataaacata tccttaaaat taatgatttt
    18601 taggaagtat ttcaatccaa taattatctg ggtccgctat aaaataaatt cccatacttt
    18661 cattttcaaa acatatgcaa tctaaatctt tatgaagttt ttttgcctca tcaaactcct
    18721 caacttctaa agccagatga aattcattgt ctcctaaatt atatggtctg tcccaatctc
    18781 taagccaagt taattctaat gtatgactag taataccatc tcctaaataa actaatataa
    18841 aacttccatc ttctgcttcc ttacgcttta cctctttaag ccctaaagct tttttataaa
    18901 aatctaaact tttttctaag ttagtaacat tgaaattgtt atggcaaaaa ttaaacttca
    18961 ttttgaaatc actctccaat ttttattttt tttatattat atcattatta taaatttatt
    19021 taaatcatat taagttaaaa atttgaatga tgatgaccaa ttaccaaatg gtggtaaatt
    19081 atttaaaaga atatgtcaaa aaaagaataa gctctttcaa atgcacaaaa aatttatacc
    19141 caagttatac ttaatcatgc aaattggcag aacttattct ttaaaactct aacttttaag
    19201 tttttctttg ataatataat ttattacttt ttttctaata tcttcagcat tataatcatc
    19261 ttctacttct atatccaaaa gttttgctat tgacctcata tcttctctag tctttgacct
    19321 atagcttgat attaattcat cttttatttt ctctggttct aaaagatatt cttcatctcc
    19381 tcttttgaaa acaacgtatt tttgagcata tgaacttcta ttatagtata tatatcttga
    19441 tatttttctt aaaatttgat tatatgaaag tcgcttgtta atatcaagtt ttaagtactc
    19501 tgaaaaagca aatagctctt tttttgattt aaataatgct tctttcttta tagtccttga
    19561 tattgaagtt acactttctt gatatgcaca cttttttaac atttttaaat atactctatt
    19621 aagaccaata ctttgattga atttttttat attattatta ataatcttat ccaagttacc
    19681 ttcaaagtat ttttttgctc ctttttgtga tacataactc tcaagttctt tgtgggccat
    19741 atcaagtatc atttttttta aatcctcttt atatccccta gattccatga tactccctcc
    19801 cttagctatt tatagtatat tcaaaaagct caagaaatgt gagtcattac aaaatttttt
    19861 tactttttat gtatatatta atttaaaagg ggagataata gtaatgtaaa atcaaacata
    19921 ctcaatctcc cccattttta gacctctatt actcccccaa tagaggcctt tttttagttt
    19981 atcaatttct attcaattcc ctttagttca aatcctaatt ctgctatcac ttcttttaat
    20041 ttttcaatag atatatcatc tttcatatct acagatgcac acttattttc taaatccaca
    20101 tctaaaactt caataccatc tatatcttct gttaatgcag ttttaagatg gtttacacaa
    20161 tgaccacaac tcataccttc tactaatagt ttcttcttca tttaattacc tccttatacc
    20221 ccataagggt attctatcat atagtttata ctttttaaat agaaattaat catttaattt
    20281 gattcattcg attttatatc taaattagat attccctcat atacatcaaa atcatttgga
    20341 gtaattttac cttttaatct actattactt acaatatagt tgtttctcac agctaaactt
    20401 ataaatgttg cattatcttt tatatatgtc tgtacatctt tgtatgcttt ttttgtttca
    20461 atttcagacg tagaacttgt aagtgcagac atatatccat ttagcttatc atcagtatat
    20521 ccactacttg ctataatact acttgcatct ggtataattg atagtttcca tccaactaat
    20581 gctaaatcat aatttttaga attaagagca ttttccatat ccttatctga cagctgattt
    20641 actgtactat ttattcctat agcttttaag ttttcattta ttatgtttgc agtttttatt
    20701 ctctcactat tatctctgtt tactattatc tttaagttca tttcagaaag acttttcttt
    20761 acttcctctt tttctctatt ttttttcttt tcagcttctt ttttgattct atcttcttct
    20821 gcttttttag cttttgcttc ttcttctggc gtaagcttct ttgagttctt tttagctgat
    20881 tttttctgat tttttacttt attgtcattt tgatttactg agtttatatt tgcatattct
    20941 accttagcaa gacactctat cgccttatct ttattaaact ctaaatcctt catttcacta
    21001 ttatagtatt ttgattttga attaagtgga aaattaactg gagtagcatc atccatataa
    21061 ccttcactaa taattctatc tttatctata gagtgagcaa ttgcttttct aaaattaaca
    21121 tctttaaaaa atggattatt ataattaaat aatacacact cataatctct tccttgatat
    21181 tttgttatat taaactcttt ttcttgaaac ttgcttaaat catttaatgt aacattggtt
    21241 atatcactat caagagccat aaccatagat gtccttgcct cttcattagg caccatacct
    21301 acttttatgt tcttcatggt ctttggaact tcttcatagt aatctttgtt tacagataaa
    21361 accattcctt ctctttctgt atatgattct attttatatt tcccatttcc tatcatatta
    21421 ttattcttgt catttacatc ctttgaatct aaatgatttt gagatacgat tggaaaaatt
    21481 aaactatcaa ttgaaaaagc atatctagct ttaaatttta ttgtaaaatc tttatctgat
    21541 ttaatattca cagaagctat atttttagta aattcattat atggactatc tgcacttttt
    21601 tgaattaaac caatagtata gtttacatca tttgaagtaa cattttttcc atcatgccac
    21661 tttgcatctt ttagctttat atctatactc attccatcct gagctatatt atactcctta
    21721 actaattgag gaattacatt gtagttctca tctatagtaa ataaactatc ataaactaaa
    21781 ttcattatat aaccaacaga cttatcagta ttagttatag gatttatagt ttttggagtt
    21841 accattgtta agtttatata cttagcatct atagaacttt gactttgtaa acttgaatca
    21901 ctactacttt gttttgtttt agtattacta caaccagcta ccataagagt tatagctaaa
    21961 acgactgtta agacttttat tctcttcatt agttttttcc tttcttgcca tatatcttat
    22021 tataatgttc gtaattattt tttacttttt ctacatattt tgaagtttgc ttaaaaggta
    22081 tatcatgtag attttcgcca tctttactat attcattatt ttctaaccat tttttcacat
    22141 tacctgaacc accattatat gcagctatca ctaaatctaa tttaccaaat tctttgtata
    22201 acttacttaa ataccaacag ccaagtctta tattagtctc tgggtcgaaa atatccacat
    22261 tttttaaatt tagttcctct gctccccaat ctctagttat atctaatatt tgcattaatc
    22321 cttttgcttc cttttttgaa acagcagaac tattaaactt actttcggct ttaataacac
    22381 tgtaaactat attttcatct aaattaaatt cttttgaata cttttctaca tactctgaat
    22441 attttttagg atataaaaat ttatgtatta ctttgctttc cattaatagt gccccaaata
    22501 agattataaa aatagataga atcaatactt tcttactatt cacgccaatt ctccttcatg
    22561 tactcaataa atttatgtgc tttactttct aattcagtta ttgttcccga attatctatt
    22621 atataatcac catatttgga tttttcttcc tgactcatct gtgaatttat cctactaagt
    22681 gcttcctgct tactacaatt atctctcttt tgtattctac taatttgtac ttcttgtttg
    22741 caagtaacta ctaatagctt gtcaaccata tctaaaaaac cactttctac caatattgct
    22801 gcatctaata ctactatatt ttcgcctttt tttctcaatt cttctatttc acttattatt
    22861 ttttctctta ttcgcggatg agtcaagcta tttaattcct taagtttttc ttcatcgcta
    22921 aaaactattt tcccaagagc ttttctattt agagttccat catcattttt tatactttgc
    22981 ccaaaatgga caaatacttt ttccaataac aacttatcct caaatatctt cctggatata
    23041 atatcagcat ccactataga aatattaaga tttctaaata tatttgatag gctgcttttt
    23101 ccacagccta ttcctcctgt aagtcctaat atcaacataa aatcacctac tttgtctcat
    23161 accaagaact acctacattt aaatctacat ctaagtgaac atccatattt acagcatttt
    23221 ccatttcttc ttttactatt ttcttaacta tatctatttc atcatcaatt gcttcaacta
    23281 tcaactcgtc gtgaacttgt agtataagtt ttgatttcaa attgttctct tccaattttt
    23341 tatgaacatt tatcatagca atttttatta tatctgctgc acttccttgt attggtgcat
    23401 tcattgcaaa tctctttcct ctatttcttt ccataaaatt gcttgacttt atttctggta
    23461 tatatcttct tctgtttaaa attgttacag catatccatc ctcactagcc ttttctactg
    23521 ttgtgtccat aaactctttt attttatggt atttagcaaa ataactttcg atatattctt
    23581 tcgcctttgg aacaggtata tttaaatcat cagctagacc aaagtcactt ttaccatata
    23641 taataccaaa gtttacagct ttagctgcac ctctcaattc actagtaaca tcttccatag
    23701 gaacattaaa aacttgtgaa gctgttttgg tatgaatatc ttcatgatgt ttaaatgctt
    23761 ctatcatatt ttcatcctga ctcatatgtg caagaactct tagctctact tgtgagtaat
    23821 cggcatcaac caacttacag ttatcatctg caataaacac ttttcttatt tttctaccca
    23881 tttccatttt tataggtata ttttgaagat ttggttctgt agatgaaatt cttcctgtag
    23941 tcgttatagt ttgattaaat gatgaatgta ttctattact ttctggattt attatattta
    24001 aaagtccttc aacgtaagta gaatttaact ttactatttg tctatactca gttatcttat
    24061 ctattattgg atgcttatct cttaattttt ctaaaacatc tgcattagtt gaatatccag
    24121 ttttagtttt tttaattatt ggaagctcta atttttcaaa taatataaca cccaattgtt
    24181 ttggtgaatt tatattgaat ggctctcctg ataattcata aatttccttt tctagtgtag
    24241 ctattatttc tttaaactct acactcaatt catccaatat acttttgtct acttttatac
    24301 cttcatactc catatgacct aagacttcta ctaaaggcat ctctacatga taaaaaagac
    24361 catccatact catatccatt agacttcttt ccatcatagg aattgtttct ttaactgtgc
    24421 atattatttg acccatatac tctgctaaat cctcaaattc taaatcctca tagtttttag
    24481 atttaacacc ctttcctaat aattcttctt ttgatttaac cttttttgat aaatacttca
    24541 tagctattgc actacaatca tatgaggtag atgaagttga atctattaag tactcagcta
    24601 ttgttatatc aaaatacata ttttctaagt ttatattata tggttttaaa gatatataat
    24661 catcttttaa attatatcca tactttttaa tttcattatt tgaaaatata ctatctaact
    24721 ttgacacttc atttgaatct acataatata ttttttctcc atctacactt ataaaaatat
    24781 atattatatt tttttcaagt atattacctt gtcctctaac agtttttaaa ataacttgtc
    24841 cttttttgtt tacttcatct ataaattttt ctgtatcttt taattttaat atttctattt
    24901 tttcttctac gtgttctgtg tcatctgaat cgaccaaatc aactaatctt gataagagac
    24961 ttgtaaatcc aaagtatcta aatttatcta aaagttcatc tttatcataa tcgccaaaaa
    25021 ccatactatc taaatccact tctattggca catctcttat aattgttgca agtcttttac
    25081 tttgaatagc tatttcttta ttttcctcta ttttcttttt tacactacct ttaagcttat
    25141 ctgtattctc tattaaattc tctatactag aaaactcttt tataagcttt atacctgttt
    25201 tttcaccgat tccaggtact ccaggtatat tatctgactt atctcccata agacctttta
    25261 aatcaataaa ttgtgctgga gtcatctcgt atttttcaat tacagaatta aagttgtatt
    25321 cttcaacttc tccaactcct ttttttgtta taagagtagt tgtattatct gaagcaagtt
    25381 gaatagcatc tttgtctcca gttactatgt ataccttata cccattattt tctgcttttt
    25441 tagaaacagt tcctataatg tcgtctgcct catacccatc tatttccaat ctatttattt
    25501 tgaatttatc taaaaggtct tttaatggct gtaattgttc tgccagttca tctggcattc
    25561 cctttcttcc agccttatac tctttatatt ctaaatgtct aaatgtaggt gcttttctgt
    25621 caaaggctac acttatatgt gttggtttat atatatctat cattttaaat agcattgttg
    25681 taaatccata tattgcattg gtctttaacc cttctttatt attcatttct ggcagagcat
    25741 aaaaggctct gtttattatt gaatttccat ctattataat taattttttt tccaaattat
    25801 atcactccta aaaattatct atttttgtaa catatatatt atataccaat atagtgttat
    25861 ttaatattcc cataataata atagtatatt aaaattaaat atgttgcaaa caatatattt
    25921 ttttgctata ataatataca tgacatgccc gaatggttga attggtaaat acaatggatt
    25981 caaaatcaac tgtgttgatt cataagtttt tataatgttt tacagtttga tgagtatttt
    26041 gtattcaata ttcctcttgc atattcttgg aaaatctggt aggatataca agtagtttgg
    26101 atttaaaata taagccgctg tggcggaact ggcatacgca tacgactcaa aatcgtacgg
    26161 gaaaccatat gggttcgagt cccatcagcg gcaccaataa aaaaaactct ttagtatata
    26221 ctaaagagtt tttttattgt aatattcttt catctttatt tctagccttg tataagtata
    26281 aataccatag tcataacttc ttgaactcct aaaatatatc taaacaaagt aggatttttt
    26341 atgtattcac ttaataaatc catacttctt atcatattga ttttttccca atctctaaaa
    26401 attttgatac aaataaaaaa tacagagaac tacttgatat actagaaggt tattctgtac
    26461 catttggtat tgctgtaggt gttccagatg gaaatttacc aaatgctaga gatgtagttt
    26521 ataaagtttc tagagtttag gataaagtgt tgcaacaatc aattagttac ttgaagagtt
    26581 aaatcagtaa tatacaaata aattaatgga agaggaagc
//
LOCUS       DAMJWP010000030        26170 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.031, whole genome shotgun sequence.
ACCESSION   DAMJWP010000030 DAMJWP010000000
VERSION     DAMJWP010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26170
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.031"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     repeat_region   570..926
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=570..598
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaat"
     gene            1165..1353
                     /locus_tag="PC700_001115"
     CDS             1165..1353
                     /locus_tag="PC700_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580434.1"
                     /translation="MKIITDSSRADYFKQRRQNKKTFSVLLDREKVEKIEEHLKKQNK
                     TKTIWLEEKINEELEKEE"
     gene            1472..2338
                     /locus_tag="PC700_001116"
     CDS             1472..2338
                     /locus_tag="PC700_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
                     /protein_id="HDF2580435.1"
                     /translation="MKNLIVKEFNGSQIYTFMWKEKSCWIANQIVGLFDYADVSKTIQ
                     DCIKAEDFEIEQEYDVLKGNEFNDFVTTLNVVANNIISNKARSITIFYEDGLYGFLQY
                     TDKPIGVQFRKWLRREVLPSIRQTGAYITNNANPEKLREKASEIEKLQLAYNSTSMLK
                     ELLDGAGFDNKSKLLTAKTLYKKAGIDLPIEINEEEHYFDTKQIASKLKIYSKSNKPA
                     QLAVCEIIKKIDLEENEVKGVWETNGSWTGTVNKYTKSVIDKVRNWIEENNRPTKIAG
                     EKKNYHVVYKIE"
     gene            3203..3730
                     /locus_tag="PC700_001117"
     CDS             3203..3730
                     /locus_tag="PC700_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HDF2580436.1"
                     /translation="MREGKRRRGCLFWFILIILFSGVVGAIAWNSTNNESTEKQKEDL
                     TKYIGEEGSIGDLKLTVNSISKASEISVASGYLAYTPDSGKYGIINLTIKNQTKESQS
                     FMLNSFTLIGPDDSKYVPSLLIDVGNKYITMDTVNPNLDVTGNIAFEIPKDLLVSDCK
                     LKYSGSEQEFILKEK"
     gene            3868..4635
                     /locus_tag="PC700_001118"
     CDS             3868..4635
                     /locus_tag="PC700_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4428 domain-containing protein"
                     /protein_id="HDF2580437.1"
                     /translation="MGLFGKKKADICCICNTEIGVLNIEDGWICNSCFKEYCDALSMT
                     KAPKILRKLDIEKTISSTKKNNELQKIFNTTNNIENYIEFDEDNKKWLVSKKSINDKK
                     TPIIHSYEDIVKFELLENGETVTKGGIGRALAGGILFGEAGAIVGGITGKKTTRKVVD
                     TFKIKITINNIDNPIEYIELINKKTKTNSSAYEKAYKDAHKILSTLSAITQSIKETDN
                     INTKSVADEILKYKNLLDMEAITQDEFNTKKKELLNL"
     gene            4701..7073
                     /locus_tag="PC700_001119"
     CDS             4701..7073
                     /locus_tag="PC700_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HDF2580438.1"
                     /translation="MATIQTSIKIFDGMTPAFRNMTTSINTTINSLERLQQRLHNPLN
                     TGGIQASQQSLNNIENILTRIEQKIGRNTNEQENFNNKIKQGSEAGSLLVSKLKSLAG
                     IYIGIRGIESITKAADTIASTKARLNLMNDGLQTTEQLNKMIYLSAQSARASYADTAA
                     QVAKLGILAGDAFGSSAEVIKFTELMNKAFVIGGTSANEASAAMYQLTQAMGAGKLQG
                     DEFRSIMENAPLLATKIADAMGKTKDQLKELSSSGAITADVIRNALFKASDEIEKKFA
                     SMPVTFSQALTMMKNDAYMIFGQTLGKISGALQSVRFSEIVVSIRNVMIAISSNIYDT
                     LNIIKNILNSEFFSNLVQGFTFCAVVITKSLGSIVNTALNIVNIFAQNWSIIKPIVFG
                     VASVFILFRGVLLATKIATIGNVIANVAHAASSSLSALMTNIQAAALMRSNGATLSAT
                     IATWGLNAALLACPITWVVLGILAFVVVVFVAVAAVNKFAGTSLTALGVIVGAVFAAV
                     AAIQNVMIWLLNGCIAVNEGITNGWNQCVYLMKQAIAKGVIFIIEKMASLNNSVNNAG
                     NALGKAFIDGANIAIRGVNKLIDLINKIPGINIGKVGEATFTPVKADNSYIKQQIDSL
                     NRWVGDAPEKVKLERMGYKDIGAAFQKGNALGTKWQNAITDKFKDTFDINKMLEDAKK
                     KLGLDDLWNKQNPLNNLGGFGGDLGKNVKDTAGNTAKMAKTMDKSQEDLKYLRDIAEQ
                     ETINRFTGVNIKIDMNNTNNISKDTDVDGIVNVLTEKLNDAMVVSAEGIV"
     gene            7090..7779
                     /locus_tag="PC700_001120"
     CDS             7090..7779
                     /locus_tag="PC700_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDF2580439.1"
                     /translation="MAYDFYLDGVQLPIAPPKLEVKVTNKNKTVDLINTGEVNILKKE
                     GLSEISFEAEFTHNKLPFCRGQFRDVQFFLSKLELLKTDCKPFQFIVSRELGNKVLFN
                     TNIKVSLEEYAISEDADNGSDTKVAIKLKQYRDYSTKKLVLAPPKNETGRPNVKIEPK
                     RVDSVNATNTKTYTVKAGDSLWSICQKQLGNGSLYKKVYELNKSMMDKANKGKNLSKY
                     TIYKGQVLKLG"
     gene            7772..9664
                     /locus_tag="PC700_001121"
     CDS             7772..9664
                     /locus_tag="PC700_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF2580440.1"
                     /translation="MVDELVLANDRDVRLVIAHWEDFYEPAVIDGITWEIERRGTPSK
                     LEFTIVMDDILEFCEGNSVRLYYKGIGIFYGYIFQKKRDKENHIKIVAYDQLRYFKNK
                     DTYVYSNKTASELVKILAKDFNLKYNVIEDTKYKISRVEENKTLFDMILTALDDTLRE
                     KKEMYTLYDDFGRITLKNVASMKLDTVMNNDVIEDFDYNSSIDSDTYTKIKLVRDNEE
                     SGKRDVYIAQDSAHMRSWGILQMFDTVDKNMSEAEIKQKCDILLKLYNKKTKSLSLKN
                     ALGDIRVRAGCLVPVFLNLGDIELQNYMLVEKVKHTFENNSHFMDLTLVDGDEFASYS
                     SSSYSSGNTNNKDEKKNGPAQSIMKKNTGKKVPAIFTAYYPGNNAMEGGKTDCNGKPL
                     DVKSRTVAGPMNREGVKKTWYTDDFLNKHPVFEYGDKVKIILPGTAYDNKVYTVKDNG
                     GRIYVETNGTYHIDILLANASECKKFGRKNGYIIIGGDEEQTYQVEGNNQSNTNNNSK
                     EDKLISIAKSKLGCNYVYGAEGPNNFDCSGFTQWCYKQIGIKIPRTASAQSKAGKAVD
                     LKDRSKWKAGDLLCRIGGGSSNHVVMYIGNNQIIHSPQTGDVVKIESVNSYRKGKAYT
                     HVRRFI"
     gene            9678..9938
                     /locus_tag="PC700_001122"
     CDS             9678..9938
                     /locus_tag="PC700_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HDF2580441.1"
                     /translation="MSQDLLQIIKKAAMDAVETSNPMRVVFGTIESISPLRVKIEQKL
                     SIGEIFLIQTDTFKRYTDKKMGDKLVLIRMQGGQQYLILDRM"
     gene            9943..10362
                     /locus_tag="PC700_001123"
     CDS             9943..10362
                     /locus_tag="PC700_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HDF2580442.1"
                     /translation="MLPSDNLDYDIEDVSIINFDVRQEPSKTFKLHIEKSKIDGICDD
                     VEALKQTIFLILNTERYEHLIYSRNYGVELNDLIGEPISYVIPELERRITEALIQDDR
                     IENIDNFEFQNIKGKVQCRFSVHTKYGNIKAEKVVSV"
     gene            10363..11412
                     /locus_tag="PC700_001124"
     CDS             10363..11412
                     /locus_tag="PC700_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDF2580443.1"
                     /translation="MFELMTFENIIKRMLDSVPDTFDKREGSIIYNALAPVAIELTET
                     YIAMDELLDQTFVDTASYYYLEKRCKERGITPLEATHTIAKGVFNIDIPLDSRFNLGE
                     YNYVAIERISEKTYKMKCETTGPVFELGKLIPIEYIDGLETAELTEILINGEDEESED
                     SLRQRYYDSLNSQSFGGNIQNYRDEVNKIQDVGGVKVYPTWDGGGTVKLVIINSNFKV
                     PSEELVNLVQEEIDPIGHQGEGLGLAPIGHRVTVEGVTSTTINISAEITYKNGYTWEN
                     IKSIAEEAIDDYLNELNMSWEDEENLIVRISQIETRLLSIDGVLDIANTMINDVKSNL
                     TIDSNSIVVRGEVVG"
     gene            11405..12022
                     /locus_tag="PC700_001125"
     CDS             11405..12022
                     /locus_tag="PC700_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HDF2580444.1"
                     /translation="MDKEINLINYLPQILQDKEEYIKVFNVENKEIKTLHDKLKDLSN
                     DQFLEDLTISGIKRWEKIMSITPKSNESLEDRRFRIFSKYISKLPYSERFLRNWLDNV
                     VGEGNYELTINNATYNIHLESDARNQDWFEEVHSFVSNIKPCNMTLDYTRVLISKDNY
                     MNFGITTLMGQEITIYPWSPPDIETYGEIDVLTGNGVGYQEITIF"
     gene            12034..13059
                     /locus_tag="PC700_001126"
     CDS             12034..13059
                     /locus_tag="PC700_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDF2580445.1"
                     /translation="MAIDKSYYTIITDVGKAKIANASVTGNKVGFVKIQLGDGGGSEY
                     TPTESQTELKNVVWEGNIGNTTTDETAPNCIILESLIPSSVGGFMIREIGYLDDENNL
                     IAISKYKECYKPSIEQGAVVDMKVKTVLIVSNINNIELKIDPTIIFATLKDIQDLDAK
                     ISNVNTKIDTTKTELTSNIETAKTELNTRIDTENEKQNIKIDQLIAGGSNVASTQTIT
                     IDDWIDNQEGGFKATVTHGLLTQRITVSIIDATTKDNVVPDFTIIDDNSIEVRSGVKV
                     ELNVYVINGNAETHFINATVDDNRVSEMTTYSSKKIHEEIGKVAEQLTGINSNIISTV
                     NNDILPI"
     gene            13076..14602
                     /locus_tag="PC700_001127"
     CDS             13076..14602
                     /locus_tag="PC700_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580446.1"
                     /translation="MALSMSYFNLPDKRKYTKNLAFNPFAGGRQNFEWTGGDHGLNGE
                     FKETCLSCTYNGSTLNWGSGNVWVLGEYGQYTFTYNCESMHVDTQQKFPYTSNRIITI
                     KGRPVISGSDTSLGNKRKGFSVDFTVSDDTPNVNLIVRAYLDDKLIQNITPVVQNSTL
                     TATVTDSQLNSLSVDGNHKLKIQLNDGYDNFDRIFTFKKIEKGIDISTSLVTDSQAKF
                     TVTKIYSELTKIECYLDETLKETFTTDLYSEKTINYELIDNAIHTLKIVVTDAENVVE
                     EKVISISKNIMPLQPDATLQDISTKLTEIGQGVRNGKTSIINTLALKNIDASLNNTLV
                     ELSEKIKGGFDSGDASLQDLMNQLTQANNTISQLNTKYKVASGTVTSFADSTKIAYPY
                     LTDNVTKPGSWIKVSNLGFKPNIFFADFDYYDAEYKNNYKLFLFACNGVATQRGVDFS
                     SVTSFIRKSGDEYFHANGWLYSNSEGDVYFNNTGVQIPAYNFDSTQKHTYKWYAIKFI
                     "
     gene            14617..14910
                     /locus_tag="PC700_001128"
     CDS             14617..14910
                     /locus_tag="PC700_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580447.1"
                     /translation="MNVPNRIIYDQTGRTIFETGESCGDVLPHYTITELHYIDIEYGS
                     IDYTRNRVIGINIETKEPILEEIPVYITDEEKRIQELENQLLIAENEKVGGLL"
     gene            14910..15092
                     /locus_tag="PC700_001129"
     CDS             14910..15092
                     /locus_tag="PC700_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580448.1"
                     /translation="MNINNVVVRILAERILSKGLNPLKNREFQLDDVTNTEYRKAVED
                     YIIKNSGVVEGAEPTI"
     gene            15126..15692
                     /locus_tag="PC700_001130"
     CDS             15126..15692
                     /locus_tag="PC700_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580449.1"
                     /translation="MTFKELVNKVRNLVLEAKNVTIEDTESKFTSENVEGALKECIDR
                     ADEAFQEADSGKTLLSTAIGSPTTSEQTFQDYANYITGFKSNISNLETQLKSKYSIRY
                     GPIDGYDGNPFSANFGKSASYLIVYVYFRRSVYYYNPSGSSLGSNTGGSERAWITINS
                     NKTGFSVHSYDTSYESYPFTGYYIACFA"
     gene            15723..16013
                     /locus_tag="PC700_001131"
     CDS             15723..16013
                     /locus_tag="PC700_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580450.1"
                     /translation="MEEISINLLCAVAGVVISYLAFRNNSNRKIQDETETTTKLEQQI
                     TFLCENVRDIKHDVAKFNTSFLDISERVAKIEASTKQAHLRIDEIVNRIGGK"
     gene            16017..16274
                     /locus_tag="PC700_001132"
     CDS             16017..16274
                     /locus_tag="PC700_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDF2580451.1"
                     /translation="MDNLISFIPEQLLILVAALSIIGKGCKKYKQLDNKYIPVVLLIL
                     GIGFSIWMLGLNPVAVLQGVICWGVAIGINQTYKQLKDGEK"
     gene            16535..17398
                     /locus_tag="PC700_001133"
     CDS             16535..17398
                     /locus_tag="PC700_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HDF2580452.1"
                     /translation="MVEVKEEKTFDEQIDILKSRGLIINDKEDAKFVLSNVNYYRFTA
                     YLLSFKNDDGSYKEGTTFEEVYDIYRFNKEFRILLTDLLGSIEIAFRTYIAYTLAIKH
                     GACGYLERESFKDEKFYINFLTALEREKSNNSDKLFIIHHKEKYEGKLPIWVATEIMT
                     FGMLSKLYSNMLPEDTRYIKNNLCRVNTLLVKSWLQSLTQVRNQCAHYGRIYNNNFRI
                     ITIKNEYKKYNLDNKKIFSYILAMKHLTMDKLIWNSFFIKLQKLINDYNNSIDLKLIG
                     FPNNWIEILAK"
     gene            17477..18289
                     /locus_tag="PC700_001134"
     CDS             17477..18289
                     /locus_tag="PC700_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2580453.1"
                     /translation="MKICITVGHSILKSGACTSADGVVNEYQYNKSLAPVLADTFRKE
                     GHKADVIICPEKQFKTKAEEKTYKIPRVNSGGYDLLIELHLNASDGQGKGSEVLYYSN
                     KGLEYATRICNKLGTVFRNRRAKLDKGLYILNSSNPTAVLIESFFCDNKEDYEKAKKL
                     GHEGIAKLIVEGVLNKNINNEGVKQMYKHTIVYDGEVDKISATVVGWGYNDGKILICD
                     IKDYVPGQTQNLYVIGGAACEKIGSMTKEKFTMIKGNDRFDTLYKALDFINR"
     gene            18383..20782
                     /locus_tag="PC700_001135"
     CDS             18383..20782
                     /locus_tag="PC700_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF2580454.1"
                     /translation="MIFKKYIKSLLIVVSVTLIMNKSILFVNALDLESNNNIKSYKRK
                     NVKEIDISNKFVDESFRRDIVEKVLDNRDDPLTNEVASKDNYKIYESDLNKIESCLEL
                     DLHKNPDVSNPNKIDNLDGIENFRNLEKLDCSSNNLESVDLSKNIKLKYLDISGNKIL
                     NIDLKNNNLINELHCDANKLSNLDLKNNKNLVSLFFTSNELTEIDLSNNVNLENLQFL
                     NNNLKSIDLSHNKKLKSLNCTNNKLKSLDLSNNVFLKNLSCSGNNLSKLNIKGLTSLE
                     YLGCAKNKLQDLDVSTNIKLDSLLCGKNSLTKIDISKNKELNSLECSGQYIEKSIIKN
                     GENIVVDIGIENSKLKELYPQNYNISTGFLTWENLSDFPNSISYSLEYEYGPVNDTET
                     MDVNISIDKLVKCRIRYIDYDGSIINEKEISDNSSINEIQINPQRENYEFSGWRNNKD
                     EKIYTSEQVSKLVFDDSTDFIATYKRKSTGSSGSSGGTSSKEDFNIAPSKEKITGADR
                     NETSVKISQKGWNKADNIVLINDSSISDALSATPFAKSKDAPILLTKNNNLNKLTEKE
                     INRLEAKNVYIVGGLKSVDEKVVSDLKKKGLNVIRISGNDRYETSIKLAKELDKNSNL
                     SKVVVVNGEKGLADAVSMGAISAKEEMPILLTNQNDDMKDIKDLIANKNISKSYVIGG
                     ESLFNNKEVNNTLPSVTKIAGSDRTETNSKVISHFYSKDTLNDLYVAKNGMNKQDDLV
                     DALSVGVLAGKTESPVVLVGNGLDDSQKELIKNKKFKNITQIGGNGNEKAFNEVENLV
                     K"
     gene            complement(20823..21008)
                     /locus_tag="PC700_001136"
     CDS             complement(20823..21008)
                     /locus_tag="PC700_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HDF2580455.1"
                     /translation="MILLEQRKLRIGFNKSGKGSFTPRLILPMSWIKEMNISPDERDV
                     LVTFEDGKIIIEKTENE"
     gene            21703..22380
                     /locus_tag="PC700_001137"
     CDS             21703..22380
                     /locus_tag="PC700_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDF2580456.1"
                     /translation="MFKHDKALLKEVKVERPNPQYAVLMQEQLGGANGELKAAMQYLS
                     QSFRIKDPQIKDLFLDIAAEELSHMEMVAQTINLLNGHDVDYNAVNTGEIETHVLTGL
                     SPVLINSSGAPWTANYVTVTGDLVADLLSNIASEQRAKVVYEYLYRQIDDKYVKETID
                     FLLNREEAHNALFRDALNKVKDTGSNRDFGVTEDSRLYFDLSTPGPNHNTKIDINPPS
                     FEKPLKK"
     gene            22554..24590
                     /locus_tag="PC700_001138"
     CDS             22554..24590
                     /locus_tag="PC700_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HDF2580457.1"
                     /translation="MDSNRKPNNLINEKSPYLLQHAYNPINWYSWNDEAFKKAKEEDK
                     PIFLSVGYSTCHWCHVMEKESFEDEEVAEIMNRNFVAIKVDKEERPDVDSVYMTVCQA
                     MTGSGGWPMTIIMTPDKKPFFAGTYFPKYSRYNRPGVIDLLKNVSEKWNTSRDILIKS
                     GDEIIEALKDDFGVKNTEGDLSKEMLSSSVRVFKAIYDEKYGGFGNAPKFPSPQNLMF
                     LMKYYSIEKDKDVLKMVEKTLDGMYRGGLFDHIGFGFSRYSTDKKWLAPHFEKMLYDN
                     AMLTIAFLDAYKITKKELYKEIAIKTIDYVVREMKDKEGGFYSAQDADSEGEEGKFYT
                     FNPLEIIEVLGEEDGTFFNNYFDITSSGNFEGKSIPNLIKNKEYERHNEKIADLSKKV
                     FEYRKERTSLHKDDKILTSWNALMIVALTKAYSTLKNDIYLEYSNKCLNFINNNLVNE
                     SGRLLARYRDGSSDYLAYLDDYAFLIWAYIELYESTFNMKYLEKALNLNESCINLFWD
                     YEKSGFYIYGKDSENLIARPKDLYDGAIPSGNSVQLYNLIRLAKITGDNRLEEMSYKQ
                     LKLYVDNVKSSPTGYSFYMLSLMFELYSTKEIICIFKEDSDLIAFKELISENFIPNAT
                     FLAKKYNEENTIIGFLNNYRLKDDKTSYYVCQSNSCSQPINDLQKLKDMILGVE"
     gene            complement(24781..25347)
                     /locus_tag="PC700_001139"
     CDS             complement(24781..25347)
                     /locus_tag="PC700_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2580458.1"
                     /translation="MDYSNELKELFLMNQTYATLFTLTNKIQIEGDKYFGILTSRQYM
                     TILSILHLPEEETTLNNIARKMGTSKQNINRLVANLEKNGYVDVIPSPHDKRAINVKV
                     TDLGKKVMVTCSRTGINFMADVFHEFTKDELETLWSLLKKMYRFNGEEQDGFEEDANF
                     MEYEEIDKIKSEALEEFAKRRNRVNKND"
     gene            25631..26158
                     /locus_tag="PC700_001140"
                     /pseudo
     CDS             25631..26158
                     /locus_tag="PC700_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436568.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
BASE COUNT        10562 a         2851 c         4603 g         8154 t
ORIGIN      
        1 ataaataagt aggtatttat ttacttgaat ttcattgttt atataaaaaa aatggaaaaa
       61 tatgtaataa ttataggtta taataattgt agcaaggata ataatcgaaa gtgcgaaggg
      121 tgattatttt catattaaac gccaaattcc aaataaggaa ggaggtgaaa ttatatgata
      181 ggttttttat taagcatact agctggtgtt atatcagctt atatttatga caaaataaaa
      241 aatcacccag acgccaataa gggtgattta aaaaaataat attttcactt aacaactgaa
      301 aataatcact ctttgtagga ataaattatt tccttgcttt tattatacca caaattggta
      361 cagatattca aaaataatat ttttatgata taataaaaat gtagagattt tgcagtgagc
      421 gatatttgtg ataaattgaa gtttaacagt tgcaatataa ggcgttgagg gtgtgtgata
      481 aatgttatca attgcactac tcatggttca ctgcaaattt aagagagttg tatatgtgta
      541 ggtattgaaa atactcaact tattttgggg ttttagatta actatatgga atgtaaatgt
      601 tgtaagaagt atcattctat tttttaatct ttctgtttta gattaactat atggaatgta
      661 aatttcagtg agaataagct ttattgtcga tgtaacactc gttttagatt aactatatgg
      721 aatgtaaata gtacatataa tgagtcttta acatcagtta tgaaaggttt tagattaact
      781 atatggaatg taaatgattg tactttagcg tctgcactag ctttgtctat cgttttagat
      841 taactatatg gaatgtaaat tattttacag atgaacaatt acagttactt cttgaatgtt
      901 ttatattaac tatgtggatt caaaattaaa tagaaaaaag aaagcactta cttaaatagt
      961 aggtgctttt gttttgctca aattacttaa ttataaagtt gctaagtaca actttttaga
     1021 attatacaat ataaaaatgc actaaagtga ttgattttgc tcaagttggt cggttgagta
     1081 aaataattag aaaaaaatgt taaaaaactc ttgaaaagtg tcgcgataca atgtataatt
     1141 atattatcgc gatacagaaa agaggtgaaa attattactg atagcagtag agcagattac
     1201 ttcaagcaga gacgacagaa taaaaaaact tttagtgttc tactagatag agagaaagta
     1261 gagaaaattg aagaacattt aaaaaagcag aacaagacta aaactatttg gcttgaagaa
     1321 aagattaatg aagagctaga aaaagaggaa taaaaaataa gagacgttct ccccgaccaa
     1381 agattgaaca tcccttattg acgtatatta tatacactaa ctatagtata cgtcattcct
     1441 tgaaaaaatt caattaagga gtgtaatagt tatgaaaaat ttaatagtaa aagagttcaa
     1501 tggaagtcaa atttatactt ttatgtggaa agaaaaatct tgttggatag ctaatcaaat
     1561 agttggatta ttcgattatg ctgatgtatc taaaacaata caggattgca taaaagcaga
     1621 agactttgag attgaacaag agtatgatgt attgaaagga aatgaattta atgattttgt
     1681 aactacttta aatgtagtcg caaataatat aattagtaat aaagctagaa gtataactat
     1741 tttttatgaa gatggtttat atggattttt acaatacaca gacaaaccta ttggtgtaca
     1801 gtttaggaaa tggcttagac gagaagtttt accaagcata agacaaactg gtgcatacat
     1861 aactaacaat gctaatcccg aaaaactaag agaaaaagca agtgagattg agaaattaca
     1921 gttagcttac aatagtacat ctatgttaaa agaactatta gatggtgcag gctttgacaa
     1981 taaatccaaa ctattaacag ctaaaacatt atataagaaa gcaggaattg atttaccaat
     2041 agagataaac gaagaagaac attattttga tacaaagcaa atagcatcta aactgaaaat
     2101 atattctaag agtaataaac cagcacagtt ggctgtttgt gaaattatta aaaagattga
     2161 tttagaagaa aacgaagtca aaggcgtttg ggaaactaat ggttcttgga ctggtactgt
     2221 aaataaatat acaaagagtg taatagataa ggttagaaat tggatagagg aaaataatag
     2281 acctactaag attgcaggtg aaaagaagaa ttatcatgtg gtttataaga ttgagtaaat
     2341 ttataagatt gtattaaata atttagttta gttttggggg gattaataca atgcatgaga
     2401 atttacttga tagtatagat attgaaaaga gaaaagaaga atttagaatt aaacttttga
     2461 aaataagaga aacagatata gatatatata ataagataga aagtatagta tataaacttt
     2521 cttagaaaaa attagagaaa aataattaaa taaatagata aagcacttgg atatttcact
     2581 gtttcaagtg ctttatatgg taaaaaatgg tataataaaa tataggaatt ttgcagtgtt
     2641 cgatttttct actcaagtat agtttaacaa ttggaataca aggcattgag aaggtttgat
     2701 aagtgttatc aattgcacta ttacccgctc actgcaaatt tgagaaagtt atatgtgtgt
     2761 aagtcttgga aatacacaat ttattttggg gttttatatt aactatatgg aatgtaaatc
     2821 tttgtaatgg tagtgtattt aagattgaaa catcaagttt tatattaact atatggaatg
     2881 taaatcaaca ttagtagttg tctttataca catagcatca cgttttatat taactatatg
     2941 gaatgtaaat acagctccca agacatacaa cgaatctgta acatcagtgt tttatattaa
     3001 ctatgtggta tgtaaaagac ttatttacag ctttatttgc taaatcagaa ggttttatat
     3061 taactatgtg gacttaaaat taaaaataat taaaaaacac ttactaatgt aggtgttttt
     3121 ttattgaaag gaagtgatta taatgtaaaa attttgaaga tatagtataa tattcttata
     3181 aaatataatt tagggggata ttatgagaga aggaaagaga agaagaggat gtctattttg
     3241 gtttattctt attattttat tttctggagt tgttggagca atagcatgga acagtactaa
     3301 taatgaaagc actgaaaaac agaaagagga tttaactaaa tatattggtg aagaaggtag
     3361 tataggagat ttaaaattaa ctgttaatag catttcaaaa gctagtgaaa tatcagtagc
     3421 atctggttat ttagcataca ctccagatag tggcaaatat ggtattataa atttaacaat
     3481 aaaaaatcaa actaaggaaa gccaatcttt tatgctaaat tcattcacat taataggacc
     3541 agatgattca aaatatgttc catctttatt gattgatgta ggaaataaat atataactat
     3601 ggatactgta aatcctaatt tagatgtaac aggtaatatt gcatttgaaa ttcctaagga
     3661 tttactagtt tctgattgta aactaaaata tagtggttca gagcaagaat ttatattaaa
     3721 agaaaaataa tatatagatt taaaaatatg aagcatttac tttaaataag taagtgcttt
     3781 tttaattaaa aggaggtgat aataatgtaa aaattttact tatatagtat aattacatta
     3841 taaaattatt gtactggggg gaatcttatg gggttatttg gaaagaaaaa agcagatata
     3901 tgttgtattt gtaatactga gataggagta ctgaatattg aagatggttg gatatgcaat
     3961 tcttgtttta aagaatattg tgatgcactt tctatgacta aagcacctaa aattttaaga
     4021 aaattagata ttgaaaagac tatatcatca actaaaaaaa ataatgaact tcaaaaaata
     4081 tttaatacaa ctaataatat agaaaactat atagaatttg atgaagataa taaaaaatgg
     4141 cttgtgtcta aaaaaagtat aaacgataaa aaaactccta tcattcattc ttatgaagat
     4201 atcgtgaaat ttgaacttct tgaaaatggt gaaactgtaa ctaaaggagg aataggaaga
     4261 gctttagcgg gaggaatttt atttggagaa gcaggagcta tagttggagg gataacaggt
     4321 aaaaaaacaa caagaaaagt tgttgataca tttaaaatta aaataacaat taataatatt
     4381 gataatccta ttgagtatat cgaattaatt aataaaaaaa caaaaactaa ttctagtgct
     4441 tatgagaaag cttacaaaga tgcccataaa attttatcaa cattatctgc tattacacaa
     4501 agcattaaag agacagacaa tataaatact aaatctgtag cagatgagat attaaaatat
     4561 aagaatcttt tggatatgga agctattaca caagatgaat ttaatactaa gaaaaaagaa
     4621 ttattgaatt tgtaatataa taaacactta ctaatgtagg tgttttttta tatggaaatt
     4681 tatgaaagga gagtgagaaa gtggcaacga tacaaacttc aataaagatt ttcgacggaa
     4741 tgacacctgc ttttaggaat atgactacat ctattaatac gacaattaat agccttgaaa
     4801 ggttacaaca aagattgcat aatccgctta acacaggtgg gatacaagca tctcaacaaa
     4861 gtttaaacaa tattgaaaac atattaacca ggatagaaca gaaaattgga agaaatacaa
     4921 atgaacaaga aaactttaat aataagataa agcaaggaag tgaagcaggt tctctattag
     4981 tatctaaact caaaagtttg gctggcatat atattggaat aagaggtata gaaagtatta
     5041 caaaagcagc agatacaatt gcaagtacaa aagcacgttt aaatttaatg aatgatggat
     5101 tacagacaac agaacaactt aataagatga tttatttatc tgctcaaagt gcaagagcta
     5161 gttatgcaga tacggcagca caagtcgcta aacttggaat attagcagga gatgcttttg
     5221 gaagttcggc agaggtgata aagtttacag aacttatgaa taaagctttt gtaattggag
     5281 gaacatcagc aaatgaagct agtgcggcta tgtatcagct tactcaagct atgggtgcag
     5341 ggaaacttca aggtgatgaa tttcgctcta taatggaaaa tgcaccttta ttagccacta
     5401 aaatagctga tgcaatggga aaaactaaag accaattaaa ggaattgtca agtagtggag
     5461 caataacagc agatgttata agaaatgcac tatttaaagc ttctgatgag atagaaaaga
     5521 aattcgcaag tatgccagtc acttttagtc aagcacttac tatgatgaaa aatgacgcct
     5581 acatgatttt tgggcaaacc ctcggaaaga taagtggagc attgcaaagc gttagattta
     5641 gtgaaattgt tgtatctata agaaatgtta tgattgcaat atcttcaaac atttacgata
     5701 cattaaatat tataaaaaat attttaaata gtgaattctt ttcaaactta gtgcaaggat
     5761 ttacattttg tgcggtagta ataactaaaa gcttaggttc tattgtaaat actgcattaa
     5821 atatagttaa tatatttgca caaaattgga gtataataaa acctattgta tttggtgtag
     5881 cttctgtttt tatattgttt agaggggttt tgctagcgac taaaatagct acaataggta
     5941 acgtaattgc gaatgttgct cacgctgctt cctcttcact atcagcactt atgactaata
     6001 tacaagcagc agcactgatg cgttcgaatg gagctacttt atcagcaaca atagccactt
     6061 ggggtttaaa tgcagcttta ttagcttgtc caatcacatg ggtagtatta ggtattctag
     6121 catttgtagt agtagttttt gttgcagtag cagcagtaaa caaattcgca ggtacaagtt
     6181 taactgcctt aggagtaatt gtgggtgctg tatttgcagc agtggcagca atacaaaatg
     6241 ttatgatttg gttattaaat ggctgtatag ctgtaaatga aggcattaca aatggttgga
     6301 atcaatgtgt atatctaatg aaacaagcta ttgcaaaagg tgtaatcttt ataatcgaga
     6361 aaatggcatc tctaaataat tctgtaaata atgctggaaa tgcacttggg aaagctttca
     6421 tagatggggc aaatatcgca atacgaggtg taaacaaatt aattgaccta ataaataaaa
     6481 taccagggat aaatattggt aaagtaggag aggcaacgtt tacgccagtt aaggcagata
     6541 atagttacat caaacaacag attgatagct taaacagatg ggtaggagac gcaccagaga
     6601 aagtaaaatt ggagcgaatg ggatacaaag atattggagc agcatttcaa aaaggaaatg
     6661 cacttggaac taaatggcaa aatgctataa ctgataaatt taaagatact tttgacatta
     6721 ataagatgct agaagatgca aagaaaaaat taggattaga cgatttgtgg aataaacaaa
     6781 atcctttaaa caaccttggt ggatttggtg gagatttagg aaaaaatgta aaggacacgg
     6841 caggcaatac tgcaaagatg gcaaagacta tggataaaag tcaagaggac ttaaaatact
     6901 taagagatat agcagagcaa gaaacaatca atcgattcac aggagtaaac attaaaatag
     6961 atatgaacaa tacaaacaac ataagtaaag atacagatgt tgatggaata gttaatgtct
     7021 taactgaaaa actgaacgat gctatggttg tatcagcgga aggaatagtt taggaaggag
     7081 agtgagaaaa tggcttatga tttttaccta gatggagtac aactaccaat cgcaccaccc
     7141 aagcttgaag tcaaagtgac aaacaagaac aagacagttg atttgataaa tactggagaa
     7201 gtaaacatat taaaaaaaga aggattatct gaaataagtt ttgaagcaga atttacacat
     7261 aataaactac ctttttgtag aggtcaattt agagatgttc aattcttttt aagtaagcta
     7321 gaattactaa aaactgattg taagccattt caatttattg tatctcgtga gttaggtaac
     7381 aaggtcttat ttaacactaa tataaaagta tctcttgaag agtatgctat ttcagaagat
     7441 gcagataatg gctcagatac aaaagttgca ataaaattaa agcaatatag agattactca
     7501 actaaaaagt tagttcttgc ccctcctaaa aatgagactg gtagacctaa tgtaaagata
     7561 gagccaaaac gagttgattc agtcaatgcc acaaacacta aaacatatac agtaaaagca
     7621 ggggatagcc tttggtcaat ttgtcagaaa caacttggta atggttcatt atataagaaa
     7681 gtatacgaac taaataaatc tatgatggat aaggcaaata agggcaaaaa cttaagtaaa
     7741 tacactattt ataaagggca ggtgttaaaa cttggttgat gaattagtgt tagcaaatga
     7801 tagagatgta agattagtaa tagctcattg ggaagatttc tacgaacctg ctgtcattga
     7861 tggtatcaca tgggagatag aaagacgagg tacaccttct aagttagagt ttacaatagt
     7921 catggatgat atactagagt tttgcgaagg aaattctgta aggctgtatt ataaaggaat
     7981 aggcatattt tatggatata tatttcaaaa gaaaagagat aaagaaaatc acattaaaat
     8041 tgttgcttac gaccagttga gatattttaa gaataaagat acttatgtat atagtaataa
     8101 aactgcaagt gaacttgtaa aaatcttggc taaagatttt aatttaaaat acaatgtcat
     8161 agaagatact aagtataaaa tatctagagt cgaagaaaat aaaacactct ttgacatgat
     8221 actaacagca ctagatgata ctttaagaga gaaaaaagaa atgtatacct tgtatgatga
     8281 ttttggaaga ataacattaa agaatgttgc atcaatgaaa ctagatactg tcatgaacaa
     8341 tgatgtaatt gaggattttg actataattc atcaatagat agtgatactt acacaaagat
     8401 taaacttgta agagacaacg aggagtcagg aaaaagggat gtatatattg ctcaagactc
     8461 agctcacatg agaagttggg gaatacttca aatgtttgat acagtagaca aaaacatgag
     8521 tgaagcagag ataaaacaaa agtgtgatat acttctaaaa ctatataata agaaaactaa
     8581 gtcattaagt ttaaaaaatg cacttggaga tattagagtg agagcaggtt gtttagtacc
     8641 tgttttttta aatctaggag atattgaatt gcaaaattat atgttagttg agaaagtaaa
     8701 acatacattt gaaaataatt cacatttcat ggatttgacc ttggttgatg gagacgaatt
     8761 tgcttcatat tcttcaagct catatagtag tggaaatact aataataaag atgaaaagaa
     8821 aaatggtcct gcacaaagta ttatgaaaaa aaatacaggt aaaaaagttc ctgctatatt
     8881 tactgcatat tatccaggaa acaatgcaat ggaaggtgga aaaacagatt gcaatggaaa
     8941 gccacttgat gtaaaatcaa gaactgttgc tggtccaatg aatcgagaag gagttaagaa
     9001 aacttggtat actgatgatt ttctaaataa acatccagtt tttgaatatg gagataaagt
     9061 aaaaattata cttcctggta ctgcctatga caacaaagta tatacagtta aagataatgg
     9121 aggaagaata tatgttgaaa caaacggaac atatcatata gatatactat tagctaatgc
     9181 tagtgaatgt aaaaaatttg gtagaaagaa tggctatata attataggtg gagatgaaga
     9241 acaaacatat caagttgaag gtaataacca aagtaataca aataacaatt ctaaagaaga
     9301 taaattaatt agtatagcaa aaagtaaact gggttgtaat tatgtgtatg gagcagaagg
     9361 tcctaataat tttgattgca gtgggtttac tcaatggtgt tataaacaaa taggtataaa
     9421 aattcctcgt actgcttctg cacaaagtaa agcaggaaaa gcagtagatt taaaagatag
     9481 aagcaagtgg aaagcaggag acttattatg tagaattggt ggaggaagta gtaatcatgt
     9541 tgtaatgtac attggaaaca atcaaataat tcattcacca caaacaggag atgtggtaaa
     9601 aatagagtct gttaattctt atagaaaagg aaaagcatac acacatgtga gaagatttat
     9661 ataagtgagg tggcaatatg agccaagatt tattacagat aataaaaaaa gctgcaatgg
     9721 atgcagtaga aacaagcaac ccaatgaggg ttgtatttgg aacaatagaa agtattagtc
     9781 ctctaagagt taagatagaa caaaaactat ctattggtga aatttttcta atacaaacag
     9841 atacatttaa aagatataca gataaaaaaa tgggagataa attagtctta attcgtatgc
     9901 aaggagggca acaatacttg attttagata ggatgtgata aagtgttacc aagtgataat
     9961 ttagattatg atattgaaga tgtatcaata attaattttg atgtaagaca agagccaagt
    10021 aagacattta aacttcatat agaaaagtct aagattgatg gtatttgtga tgatgtagaa
    10081 gcattaaaac aaaccatttt tttaatttta aatactgaaa ggtatgagca tcttatttat
    10141 tctagaaatt atggtgttga attaaatgat ttaattggag aacctatttc atatgtaata
    10201 cccgaacttg aaagaaggat aacagaagca ctaattcaag atgataggat tgaaaatata
    10261 gataattttg agtttcaaaa tataaagggt aaagtacaat gtagattttc agttcataca
    10321 aaatatggaa atataaaagc agagaaggtg gtgagtgtat aattgtttga gttaatgaca
    10381 tttgaaaata taattaaaag aatgttagat agtgtaccag atacttttga taaaagggaa
    10441 ggttctataa tatataatgc tcttgctcct gttgctatag aacttacaga aacatacatt
    10501 gctatggatg aattactaga ccaaacattc gtagatactg ctagttatta ttatttagag
    10561 aagagatgta aagaaagggg aataacacca cttgaagcca ctcatacgat tgctaaagga
    10621 gtttttaaca tagatattcc acttgattct aggtttaatc taggagaata taactatgta
    10681 gcaattgaga gaatatctga aaaaacatat aaaatgaaat gtgaaactac tggacctgta
    10741 tttgagttag gaaaactaat acctattgaa tatatagatg gtcttgaaac tgctgaacta
    10801 actgaaatct tgataaatgg agaggatgaa gagtcagaag atagtttaag acaaagatat
    10861 tatgatagcc taaattcaca gagttttggc gggaatatac aaaactatag ggatgaagtt
    10921 aacaaaatac aagatgttgg aggagttaaa gtttatccaa cttgggatgg aggtgggact
    10981 gttaagttag taataattaa ctctaatttt aaagtgccaa gtgaagaatt agtaaactta
    11041 gttcaagaag aaattgaccc aattggacac caaggagaag gcttaggatt agcaccgatt
    11101 ggacaccgag tcacagttga aggagttaca agtacaacta taaacatatc agcagaaata
    11161 acatacaaaa atggctacac ttgggagaat ataaaatcaa ttgcagaaga agcaatagac
    11221 gactatttaa atgaacttaa catgagttgg gaagatgaag aaaacttaat agtccgtata
    11281 tctcaaattg aaactagatt acttagtatt gatggagtat tagacattgc aaatacaatg
    11341 ataaatgatg ttaaatctaa tctaacaata gatagtaaca gcatagtagt gagaggtgag
    11401 gtagttggat aaagagatta atctaataaa ttacttgcca caaattctac aagataaaga
    11461 agaatatata aaagtattta atgtagaaaa taaagaaata aaaacactac atgataaatt
    11521 aaaggaccta tcaaatgacc agtttttaga ggacctaact ataagtggta taaaaagatg
    11581 ggaaaagata atgtctataa ctcctaaaag taatgagagt ttagaagata gaaggtttag
    11641 gatttttagt aaatatataa gtaaattacc ttactcagag agatttttaa ggaactggct
    11701 agataatgta gttggagaag gcaattatga attaactatt aataatgcta cttataacat
    11761 acaccttgag agtgatgcta gaaatcaaga ttggtttgag gaagttcatt cttttgtaag
    11821 taatattaag ccatgtaata tgactttaga ttacactaga gtgcttataa gcaaagacaa
    11881 ttatatgaat tttggtataa caaccctaat gggtcaagaa ataactatat acccttggag
    11941 tccaccagat atagaaactt atggagaaat tgatgtatta actggcaatg gagttggata
    12001 ccaagagata acaatatttt aggaggtgat atattggcta tagataaaag ttattacact
    12061 ataattacag atgtaggaaa agcaaagata gcaaatgcaa gtgtcacagg taataaagta
    12121 gggtttgtaa aaattcaact tggtgatggt ggaggaagtg agtatactcc tactgaaagt
    12181 cagacagaac ttaaaaatgt ggtatgggaa ggcaacatcg gaaatacaac cacagatgaa
    12241 actgcgccaa actgcataat attagagagt ttaataccat caagtgtagg tggatttatg
    12301 ataagagaaa taggatattt agatgatgaa aataatctaa ttgcaatctc taaatataaa
    12361 gaatgttata aaccttctat agaacaagga gcagttgttg atatgaaggt taaaactgtg
    12421 cttattgtat ctaatataaa taatatagaa cttaaaattg accctacaat aatttttgct
    12481 acgctcaaag atatacagga tttagatgct aaaataagca atgtaaacac taaaattgat
    12541 acaactaaaa cagagttaac aagcaacata gaaactgcta agacagagtt aaacactaga
    12601 attgacacag aaaatgagaa acaaaatatt aaaattgacc agctaatagc aggtggttca
    12661 aatgttgcat ctactcaaac aataacaatt gatgattgga tagataatca agaaggtgga
    12721 ttcaaagcaa ctgtaacaca tggtttatta acacagagaa taactgtaag tattatagat
    12781 gctactacaa aagataatgt agtacccgat ttcacaatta tagacgataa ttctattgag
    12841 gttagaagtg gagtaaaagt tgagttaaat gtttatgtga ttaatgggaa tgcagagact
    12901 cattttatta atgcgactgt agatgataat agagtgtctg aaatgactac ttattcatca
    12961 aaaaagatac atgaagaaat tggtaaggta gcagagcaac tgacaggaat taatagtaat
    13021 attatatcga ctgtaaataa cgacatacta cctatataga aaggagcgtg aaaaaatggc
    13081 gttatcaatg agttatttta atttacctga caaaagaaaa tatactaaaa atcttgcatt
    13141 taatccattt gcaggtggaa ggcaaaattt tgagtggact ggtggtgacc atggactaaa
    13201 tggtgagttt aaagaaactt gcttaagttg cacttacaat ggttcaacat taaattgggg
    13261 ttcaggaaat gtttgggtct tgggggaata tggtcaatat acatttactt ataattgtga
    13321 atcaatgcat gtggatacac aacaaaaatt tccgtatact tctaatagaa taataactat
    13381 aaaaggtaga cctgtaatat ctggttcaga tacttcactt gggaataaaa gaaaaggatt
    13441 tagtgtagat tttactgtaa gtgatgatac tccaaatgtt aatttaatag tacgtgctta
    13501 tcttgatgat aaattaatac aaaacattac tcctgttgta caaaattcaa ctttaacagc
    13561 aacagtaaca gatagtcaac taaactcttt atccgtagat ggaaatcaca aactaaaaat
    13621 acaattaaat gatggatatg ataattttga tagaattttt acttttaaaa aaatagaaaa
    13681 gggaattgat atatcaacat cattagtaac tgatagccaa gctaagttta ctgtaacaaa
    13741 aatatacagt gaattgacaa aaatagagtg ttatttagat gaaacattaa aagagacttt
    13801 tactacagac ttgtattcag aaaagactat aaactatgaa cttatagaca atgcaataca
    13861 cacattaaaa atagttgtaa ctgatgcaga aaatgttgta gaagaaaaag ttataagtat
    13921 aagtaagaat ataatgccat tgcaacctga tgcaacatta caagatatat caactaagtt
    13981 aacagagatt gggcaaggcg ttagaaatgg taaaacaagc attataaata ctttagcatt
    14041 aaagaatata gatgcaagtt taaataatac actcgttgag ttatcagaga aaattaaggg
    14101 aggttttgat agtggagacg ctagtttaca agatttgatg aatcaattaa cacaagctaa
    14161 taatactata tcgcagttaa atactaagta taaagttgct agtggtacag ttacttcttt
    14221 tgcggatagt actaaaattg cttatccata tctaaccgac aatgttacta aacctggttc
    14281 ttggattaaa gttagtaatt taggctttaa acctaatatt ttctttgctg attttgatta
    14341 ctatgatgct gaatataaaa ataattataa acttttcctt ttcgcttgta atggtgttgc
    14401 cacacaaaga ggtgttgatt tttcaagtgt tacatctttt atcagaaaaa gtggtgatga
    14461 gtattttcat gctaatggat ggctatatag taactcagag ggggatgtct attttaataa
    14521 tactggcgtt cagattccag cttataactt tgattcaact caaaaacata cttataaatg
    14581 gtatgctatt aaatttattt aaaaggagga aaaaacatga atgttccaaa ccgaataata
    14641 tatgaccaaa caggtagaac aatctttgaa acaggcgaat cctgtggaga tgtattgcca
    14701 cattatacaa taacggaatt gcattatata gatattgaat atggaagtat agattataca
    14761 aggaatagag ttataggtat aaatatagaa acaaaagaac cgattttaga agaaatacca
    14821 gtatatataa cagatgagga aaagagaata caagagttag aaaatcaatt attaattgca
    14881 gaaaatgaga aagtaggagg attattataa tgaatataaa taatgttgta gtaagaatat
    14941 tagcagaaag aatattaagt aagggattaa atcctctaaa aaatagagaa tttcagttag
    15001 atgacgtgac taacacagag tacagaaaag ctgtagagga ttatattata aaaaatagtg
    15061 gagtagtaga aggagcagaa ccaactatat agagggttct ttttttattg aaagaaggtg
    15121 actaaatgac ttttaaagag ttggttaata aagttagaaa tcttgtatta gaagcaaaga
    15181 atgtaactat agaagataca gaaagtaaat ttacaagtga aaatgtagaa ggagcattga
    15241 aagaatgtat agatagagca gatgaggctt ttcaagaagc cgatagtgga aaaacacttt
    15301 tatcaactgc tatcggctct cctactacat cagaacaaac atttcaagat tatgcgaact
    15361 atattacagg atttaagagc aatataagca atttagaaac tcaattgaaa agcaaatatt
    15421 ctattagata tggtcctatt gatggatatg atggtaatcc tttttctgct aattttggca
    15481 agagtgcaag ttaccttatt gtctatgttt actttagaag aagtgtatat tattataatc
    15541 ccagtggtag ttctttagga agtaatacag gaggttctga acgtgcatgg attactataa
    15601 acagcaataa aactggtttt tcagttcatt catatgatac tagttatgag tcatatcctt
    15661 ttacaggtta ttatattgct tgtttcgcat aataaattat attattaaaa ctaggaggat
    15721 gtatggaaga aattagcata aatctactat gtgcagtagc aggagttgta atatcctact
    15781 tagcatttag aaataactca aacagaaaga tacaagatga aacagaaaca actacaaaat
    15841 tagaacaaca aataactttt ttatgtgaga atgtaagaga tataaagcat gatgtagcaa
    15901 agtttaatac tagtttctta gatattagtg aacgagttgc aaaaatagaa gcaagtacaa
    15961 aacaagcaca tcttagaatt gatgaaattg taaatagaat tggaggaaaa taagagatgg
    16021 ataatttaat aagttttata ccagagcagt tgctaatttt agtagctgct ctctctatta
    16081 taggtaaagg ttgtaaaaaa tataaacaat tagataataa atacattcca gtagtgttac
    16141 tgatacttgg aataggtttc tcaatatgga tgctaggatt aaatcctgtt gcagtcttac
    16201 aaggtgtaat ttgttgggga gttgcaatag gtataaatca aacttacaaa cagttgaagg
    16261 atggtgaaaa gtaatgaaat taacaaaaat attattactc acaaaaattt aaaaaatttc
    16321 attaaataga gtatactttt atagctaata agtatataat aatagtacag aactcgtaaa
    16381 gcatttatat gctcaaatat acgggacatt gatttttgaa ggaacccgcc aagcttcttt
    16441 tatagctcaa ataggcggga cagataaatt tacccactaa tattaattgg tgggttttat
    16501 tatataaaag tatatctatg aaaaggtgaa aacaatggtt gaagtaaaag aagaaaaaac
    16561 atttgatgaa caaatagata ttttaaaaag tagaggatta ataataaatg ataaagaaga
    16621 tgctaaattt gtattaagta atgtcaatta ttataggttt acagcatatc ttctaagttt
    16681 taagaatgat gatggctcat ataaagaagg aactacgttt gaagaagttt atgatatata
    16741 taggtttaat aaggaattta ggatattatt aacagatttg ttagggagca tagaaatagc
    16801 atttaggaca tacattgcat atacattagc aattaaacat ggtgcttgtg gatatctaga
    16861 aagggagagt ttcaaagatg aaaaattcta tattaatttt ttgacagcat tagagagaga
    16921 aaaaagtaat aattcagata agctttttat tatacaccat aaagaaaaat atgaaggaaa
    16981 acttcccatt tgggttgcaa cggaaataat gacttttggt atgttgtcaa aattatattc
    17041 aaacatgttg ccagaagata ctagatacat aaaaaataat ttgtgtagag tgaatacttt
    17101 attagttaaa tcttggttac aatcattaac acaggtcaga aatcaatgcg ctcattatgg
    17161 tagaatatat aataataatt tccgtattat aacaataaaa aacgaatata aaaagtataa
    17221 cttggataat aaaaagatat tttcttatat acttgctatg aagcatttga ctatggataa
    17281 attaatttgg aatagttttt ttataaaact tcaaaagtta attaatgatt ataataattc
    17341 tatagactta aagcttattg gttttcctaa taattggata gagatattgg ctaaataaaa
    17401 tagttacttt aagaagttta taaacactta ctatatgtaa gtgttttttt attgaaaaga
    17461 aggaggaaaa taaataatga aaatatgtat tacagtagga cacagtattt taaaaagtgg
    17521 tgcatgcact tctgctgatg gagtagttaa cgaataccaa tacaataaat ctcttgcacc
    17581 agtattagca gatacattta gaaaagaggg tcataaggca gatgtaataa tatgtcctga
    17641 aaagcagttt aaaactaaag cagaagaaaa gacttataaa atacctagag ttaatagtgg
    17701 aggatatgat ttacttatag aactacatct aaatgcaagt gatggtcaag gaaaaggttc
    17761 agaagttcta tattatagta ataaaggttt agagtatgca actagaatat gtaataagct
    17821 aggtacagta tttagaaata gaagagctaa attagataaa ggattatata tcttaaatag
    17881 ttcaaatcct acagcagtat taattgaaag tttcttctgt gataataaag aagattatga
    17941 gaaagctaag aaactaggtc atgaaggtat tgctaagtta attgtagaag gtgtattaaa
    18001 taaaaatata aataatgagg gagttaaaca gatgtacaaa catacaattg tttatgatgg
    18061 agaagttgac aaaatctctg caactgtagt tggttggggt tataatgatg ggaaaatact
    18121 gatatgtgat ataaaagatt acgtgccagg tcagacgcaa aatctttatg tcattggtgg
    18181 tgcagcatgt gagaagattg gttctatgac taaagaaaaa tttactatga taaagggtaa
    18241 tgatagattt gatacacttt ataaggcatt agattttatt aatagataga ttatataaaa
    18301 tattttagta tttcttgaaa atcataagta tggtaatatt tagataaata ttatttatta
    18361 ttaataggta tggggggaga ttatgatatt taagaagtat ataaaatcat tactgatagt
    18421 tgttagtgtt acattgataa tgaacaaatc aatattattt gtaaatgcat tagatttaga
    18481 gagtaacaat aacataaaat cttataaaag aaagaatgta aaggaaattg atataagcaa
    18541 taaatttgtt gatgaatcct ttagaagaga tattgttgaa aaggtattag ataataggga
    18601 tgatccatta acaaatgaag ttgcatcaaa agataactat aaaatatatg aaagtgattt
    18661 aaataaaatt gagagttgct tagagttaga tttacataaa aatccagatg tttctaatcc
    18721 aaacaaaata gataatttag atgggataga aaattttaga aatctagaaa agcttgattg
    18781 tagttcaaat aatctagaaa gtgtagattt aagtaaaaat ataaaattaa aatatctaga
    18841 tattagtgga aataaaatat taaacattga tttaaaaaac aataatctta ttaatgaatt
    18901 acattgtgat gcaaataaat tatcaaattt agaccttaaa aataataaaa atttggtaag
    18961 tttatttttt actagtaatg aattaacaga gatagattta agtaataatg tgaacttaga
    19021 aaatttacaa tttcttaata ataatttaaa aagtatagat ttaagtcata ataaaaagtt
    19081 gaaatctcta aattgtacta acaataaact taaatccctt gatttaagta ataatgtttt
    19141 tttgaaaaat ttaagttgtt caggtaataa cctgagtaaa ttaaatataa aaggacttac
    19201 aagtcttgag tatttgggat gtgctaagaa taaattacaa gatttagatg tatctacaaa
    19261 tataaaatta gattctttac tttgtgggaa aaatagttta acaaaaatag atataagtaa
    19321 aaataaagaa ttaaattcac tggaatgtag tggtcaatat atagaaaaaa gcattataaa
    19381 aaatggtgaa aatatagttg tagatatagg aattgaaaat tcgaaattaa aggaattata
    19441 tccacaaaat tataatattt caacaggttt tctaacatgg gaaaatcttt ctgactttcc
    19501 aaatagcata agttatagtt tggaatatga atatggtcca gtaaatgata cagagactat
    19561 ggatgtaaat atatcaatag ataaattagt gaaatgtaga atcagatata tagattatga
    19621 tggaagtata attaatgaaa aagaaatatc agataattct agtataaatg aaattcaaat
    19681 taatcctcaa agagaaaact atgagtttag tggttggagg aacaataaag atgaaaaaat
    19741 atatacatca gagcaggtaa gtaaattagt ttttgatgat agtactgatt ttatagcaac
    19801 ttataaaaga aaatcaacag gatcaagtgg ttcttctggt ggaacttcaa gtaaagaaga
    19861 ttttaatata gcaccatcta aggaaaaaat aacaggtgca gacagaaatg aaacatcagt
    19921 gaaaataagt caaaaaggat ggaataaagc tgataatata gtgttaataa atgactctag
    19981 catatcggat gctttatcag caacaccatt tgctaagtct aaagatgcac caatattact
    20041 tactaaaaat aataatttaa ataaattaac agaaaaagaa ataaatagat tagaagcaaa
    20101 aaatgtgtac atagtgggtg gattaaagtc tgttgatgaa aaagtagtgt ctgatttaaa
    20161 gaaaaaagga ttaaatgtaa ttagaatatc tggaaatgat agatatgaaa catcaataaa
    20221 acttgcaaaa gaattagaca aaaattctaa cctgtcaaaa gtagtagtgg taaatggaga
    20281 aaagggacta gctgatgcag taagtatggg agctatatca gcaaaagagg aaatgccaat
    20341 tcttcttact aatcaaaatg atgatatgaa agatataaaa gacttaatag ctaataaaaa
    20401 catatcaaaa tcatatgtaa taggtggaga atctttattt aataataaag aagtgaataa
    20461 tacattacca tctgtaacta aaatagcagg ttctgataga acagaaacta actcaaaagt
    20521 aataagtcat ttctatagca aagatacact taatgactta tatgtagcta agaatggaat
    20581 gaataaacaa gacgatttag tagatgcctt atcagtagga gtgcttgcag gtaaaactga
    20641 atctccagta gtattagttg gaaatggatt agatgatagc caaaaagagt taatcaagaa
    20701 taaaaagttt aaaaatataa ctcaaattgg tggaaatgga aatgaaaaag catttaatga
    20761 agtagaaaac ttagttaaat agattgaaag gtagtaactg gaaatagtta ctacctcctt
    20821 ttttattcat tttcagtttt ttcaataatt atttttccat cctcaaaagt aacaagaaca
    20881 tccctttcat caggagagat attcatctct ttaatccatg acattggaag tatcaatctg
    20941 ggtgtaaaag agcctttgcc acttttatta aatccaattc taagttttct ttgctccaat
    21001 aatatcattc ctttctacta ttaataaatg ccagcaataa taaagtaaat accaaagcaa
    21061 taagagcttt aaaagtaaaa cttattatta tattaataat atttaaaact atagtgatac
    21121 caagaataat catacaaatt ctataaaatt tattattcat attaaattag aaaatgtgtt
    21181 ataatattag aaagaagcta cctagaagga aggtattttc ctcctaggat tgaaacttat
    21241 aattctttaa tcaatcttag tattgcgagt actagattaa ttaaaactaa tgtgaaagag
    21301 aaagcatttt taattttatt aaatttgttt tctcttttat tttttcttct ttctaatctc
    21361 cccactttct tacctccttt ctatatttat attatatcat cacgtgacgt gaaagtcaat
    21421 aaatattaca tatttttcaa taaagttata ttgataaact atatttttta caaataaaaa
    21481 taaatggtga caaaaccaaa cttgccacca ttttgccacc gaaaataaat aatagaataa
    21541 atttaaatta aacataataa aacataaaca acaaaactga ccttaataaa acattgaaat
    21601 accaacactt gtaaaaaata acaacaataa acaaataacc tagaataata ttacaactta
    21661 agtaaataat aattttgaat aataatttac ggaggtattc atatgtttaa acacgataaa
    21721 gcattattaa aagaagtaaa agtagaaaga ccaaatcctc aatatgctgt tttaatgcaa
    21781 gaacagttgg gtggtgcaaa tggagagtta aaagcagcta tgcaatactt gtcccaaagt
    21841 tttagaataa aagaccctca aataaaagat ttatttcttg atatagcagc agaagagctt
    21901 agccatatgg aaatggtagc acaaactata aatttattaa atggacatga tgttgattat
    21961 aatgcagtta atacaggcga aatagaaaca catgtattaa ctggtctatc accagtctta
    22021 ataaactcat caggagctcc ttggactgct aattatgtta cagttacagg ggatttagta
    22081 gctgacttac tttcaaatat agcttctgaa caaagagcta aggtagttta tgagtacttg
    22141 tatagacaaa tagatgataa atatgtaaaa gaaacaatag atttcttact taacagagaa
    22201 gaagcacata atgctctatt tagagatgct ttaaataaag ttaaagatac aggttcaaat
    22261 agagattttg gagttacaga agattctaga ttatatttcg atttgtctac tccagggcca
    22321 aatcacaata ctaagataga tattaatcct ccttcttttg aaaaaccttt aaaaaaataa
    22381 tttgatattt tttatgttgt tttgttttag tagttataaa aattatttac tagaagaatt
    22441 aattatatga aaaattaaat atgtatagtt aaaaatgggt atacacttat tataagtgta
    22501 tatctatttg tttattgcaa aacatataat aaaataacaa atgaggtggt attttggaca
    22561 gtaatagaaa acctaataat ctaataaatg aaaaatctcc atatctttta cagcatgctt
    22621 ataatcctat aaactggtat agttggaatg atgaggcttt taaaaaagca aaagaagagg
    22681 ataaaccaat atttttaagt gtaggatatt caacatgtca ttggtgtcat gttatggaaa
    22741 aagaatcttt tgaagatgaa gaagttgctg aaataatgaa tagaaacttt gtggcaataa
    22801 aagtagataa agaggaaaga cctgatgtag atagcgtgta tatgacagtt tgccaagcta
    22861 tgacaggtag tggtggatgg ccaatgacca taataatgac accagataaa aaaccttttt
    22921 ttgcgggaac atatttccca aaatattcaa ggtataatag acctggagtt atagatttat
    22981 taaagaatgt atctgaaaaa tggaatacaa gtagggatat attaataaaa tctggagatg
    23041 aaataataga agctcttaag gatgattttg gtgtaaaaaa tactgagggt gatttatcaa
    23101 aagagatgtt gagttcatca gttagagtat ttaaggccat ttatgatgaa aagtatggtg
    23161 gttttggtaa tgctcctaaa tttccaagtc ctcagaatct tatgttttta atgaaatatt
    23221 atagcataga aaaagataaa gatgtgttaa aaatggtgga aaaaacttta gatggaatgt
    23281 atagaggagg cttgtttgat catattggat ttgggttttc aagatactct actgataaaa
    23341 agtggttggc tcctcatttt gaaaaaatgc tatatgataa tgctatgctt actatagctt
    23401 ttttagatgc atataaaata acaaagaaag agttatataa agaaattgct ataaaaacta
    23461 tagattatgt agttagagag atgaaggata aagaaggtgg gttttattca gcacaggatg
    23521 ctgatagtga aggtgaagaa ggtaaatttt atacatttaa tcctcttgag ataattgaag
    23581 ttttgggtga agaagatgga actttcttta ataattattt tgatataact agtagtggaa
    23641 attttgaagg taaaagtatt cctaatctta taaaaaataa agaatatgaa agacataatg
    23701 aaaagatagc tgaccttagt aaaaaggtat ttgaatatag aaaagaaaga acatctttgc
    23761 ataaagatga caagatactt acatcatgga atgcattgat gatagttgct ttaacaaagg
    23821 catatagtac tttaaaaaat gacatatatt tagagtattc aaataagtgt cttaacttta
    23881 taaataataa tcttgtaaat gagtcaggta gattattagc tagatataga gatggaagtt
    23941 ccgactattt agcatattta gatgattatg cttttttaat ctgggcgtat atagagcttt
    24001 atgagtcaac atttaatatg aaatatttag aaaaggcttt aaatttgaat gaaagctgta
    24061 tcaacctttt ttgggattat gaaaaaagtg gattttatat atatggtaaa gatagtgaaa
    24121 atttaatagc aagaccaaaa gatttgtatg atggagctat tccatcagga aattctgttc
    24181 aattatacaa tcttataaga cttgcgaaaa ttactggaga taatagactt gaagaaatgt
    24241 catataaaca attaaaactg tatgtggata atgtaaagag ttctccaact ggctacagct
    24301 tttatatgct ttcattgatg tttgaacttt attctactaa ggaaattata tgtattttta
    24361 aggaagattc tgatttaatt gcatttaaag aattgataag tgaaaatttt ataccaaatg
    24421 caactttttt agcaaagaaa tacaatgaag aaaacactat tataggtttt ttaaataact
    24481 atagattgaa ggatgataag actagttatt acgtgtgtca aagtaatagt tgtagtcaac
    24541 caattaatga tttacaaaaa ttaaaagata tgattttagg agtagaataa tcataatagc
    24601 gaaaaaggag atgctttaaa ataaaagtct aatcatctcc tttttttgta caaaaaacag
    24661 aaaaagagtt cctaagaaca cctaatctat gtaatgattg ttttgttaaa aaataaaaat
    24721 gacattatag ttatataaaa tattgtacta taaaaaagtg gtattataga tgcgatagta
    24781 ttaatcgttt ttatttaccc tatttcttct ttttgcaaat tcttcaagtg cttcagattt
    24841 aattttatct atttcttcat attccataaa attagcatct tcttcaaagc catcttgttc
    24901 ttcaccatta aaacgataca ttttttttag taaactccat aaggtctcta attcatcttt
    24961 ggtaaattcg tgaaatacat ctgccataaa gtttattcca gtcctactac atgttaccat
    25021 aacctttttt cctaagtctg taactttaac attaattgct ctcttatcat gaggacttgg
    25081 aattacatca acatatccat ttttttcaag attagcaact agtctattta tattttgttt
    25141 acttgtaccc atttttcttg caatattatt taatgttgtt tcttcctctg gtaaatgaag
    25201 aattgataaa atagtcatat actgtcttga agtaagtata ccaaaatatt tatctccttc
    25261 tatttgtatt ttgtttgtaa gggtaaaaag agttgcatat gtttgattca ttaaaaagag
    25321 ctcttttagt tcatttgaat aatccatgaa atatatctcc tttattttta gaagttgttt
    25381 tgtcgaaaac agaatatcat gaattaattc taatgtcaat atggtgtcaa aatatattaa
    25441 agtattactt ttttatgtaa ctattaatta aattattgta taattgtatg taaaaacttc
    25501 tatgtaccaa tgataatttc tatgaggaat taataattct taaatttttt agaaagattt
    25561 tgacttgaaa atacttgtaa tataatatat aaattaatat actgaataaa atcaatctgg
    25621 agggataatt atgaagtgta caaactttac tttcaaagga gaggaaggtt tagatatata
    25681 tacatataaa tgggaagatg aaaatataaa aaaaccaaag gcagttatcc aaattgcaca
    25741 tggaatggct gaaactgcac aaaggtatga gacttttgcc aaggtactta ctaaaaatgg
    25801 ttatatagta tatatcaatg accatagagg tcatggaaaa actgcaaaga taatagaaaa
    25861 tgttggacat ttagctgaaa aagaaggatt tagatgtctt gtagaggaca tgtatacttt
    25921 aacaaatatt ataaagaaag aaaatgaaga tttgccaatt tatctatttg gacacagcat
    25981 gggttcattt gcaagtcaaa gatatataat ggattacagt aataatttat ctggtcttat
    26041 actatgtggt tcaaatggaa agcaaggaat cattctaaat cttgctcatc tgataatcaa
    26101 tcgtgagatt aaaaagtatg gaagacgttc taaaagtaat aaaataaata atttgattaa
    26161 aaaatagcta
//
LOCUS       DAMJWP010000031        26093 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.032, whole genome shotgun sequence.
ACCESSION   DAMJWP010000031 DAMJWP010000000
VERSION     DAMJWP010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26093
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.032"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(107..448)
                     /locus_tag="PC700_001141"
     CDS             complement(107..448)
                     /locus_tag="PC700_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580459.1"
                     /translation="MLLILDNLNLNLDFDYRIIREENDDVDIFIDINYRSLDIDTDGS
                     NLFNSRIQFPFVRALILRINKNNQCMTIHLLRDIDLFSAFANFEVDYTDSIINIKNQN
                     EKVILNKSIKK"
     gene            complement(475..1146)
                     /locus_tag="PC700_001142"
     CDS             complement(475..1146)
                     /locus_tag="PC700_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2580460.1"
                     /translation="MPILEILNLGKIYGKKETAVHALKDANLKINKGEFVAIIGPSGS
                     GKSTFLHLVGGLERPSSGTIKVDGKDICCLSDKKLAKYRRQKVGFVFQQYNLIPVLNV
                     KENIELPLKLDNKKVDEEYIEDIINLLGLSERKNHLPNQLSGGQQQRVAIARALSAKP
                     SIILADEPTGNLDSKTTEEVMDLLKSSIKKYNQTLIIITHNENIARKADRIISIIDGE
                     LKLTL"
     gene            complement(1158..3743)
                     /locus_tag="PC700_001143"
     CDS             complement(1158..3743)
                     /locus_tag="PC700_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2580461.1"
                     /translation="MKDNLKIALRYIISYKARSLAIALSIILATSLIVGIGTLSRSAQ
                     QAEVDKLKRETGSDHVYFKDINKNQLEYIKSRKDIKNLGITSHYGYTDPNERLAINLE
                     YANKNYLTNQSKLIKGHLPKASNEVVVEKWILNSLGLKPEINQNITFKLYQKEKPETF
                     KVVGILEDRPIEKNKGTCEIFLNLNESKLDKFSYAYIEFNNGIDINTSIDNIVKNTML
                     DENSVGKNKMLIESTRENGTLDNSSKYTAITMSLFSGIVIYSIYVISIYQRIQEYGIL
                     RAIGSTNFKIFKFMFYELFILALIAIPIGICTGIGGAQIFNRTAGNIQFEGNVTVTPF
                     VIPDKIILLSIGSIILTILIISFFTYLKIRRISPIDSIRKTFGTDKNINKVNSLISKL
                     TLNISVTKSISAKNIFRNKKGFIIIILSMSLGGIMIIKENYKYSFSDIQNKNGQEETY
                     MNADFILTNFFLKLNQSNKADSFKDIKGLNDNQIDKIKNINGIDKVKTASILDTRIEV
                     DKLNGLDYYNIINSTPYYKDFPLFIKNKNTGKYTMKQKLRGYNDEMINSLEKYLVSGS
                     INLERMKKENLAILYVPQVSKTNKYKLSYTPGTETPAVDIKVGDTIKVKYPKGEIDQD
                     LYIKLKDNYEYLEYEFKVGAIVSYPFADNYLYSGDQGIDVITSNDYLKKLTGVDKYNV
                     VYVDINKNANVKKINTLLGEIGSESPGTTTANMLMEKENFDKMTARALTYAYGIVAVM
                     FIISVFNIINNISYNLTSRTSEFGMLRAIGISERGFKNMILYEGILYGILSSVITIVV
                     GLIIQFRMYYTYNFVSYGLGFSIDYKIYILVVLANIIVGILATYIPLRKINKISIVEA
                     INITE"
     gene            3951..4886
                     /locus_tag="PC700_001144"
     CDS             3951..4886
                     /locus_tag="PC700_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2580462.1"
                     /translation="MENNFIFPIVILTLFIFSNLIMNKYRFYKELDILKRGILTFFVS
                     CFVWILFMEGIEYIQLSLGYNFLLEGVLNIILFLILIILSFYIGFKFIRLLDNNNKQE
                     YKLQKLDSKINHKDNIIVKLRSQKHDYLKHLQVVYSLLNTGLAEDAKEYITNISNSFK
                     SKNSRYGKVSYLDAIASLKYEECLEKNISFDVYIQDFMDNLEIEPNDMSSVLLNIIDN
                     AIESLSAIQKEKKYIRLHAYEDEFQYIIAIKNNGPKIHNVESIFLDGFSTKEGQDRGF
                     GLYLVKNILDKYDYSILVNSDDFLTEFVILIPKYY"
     gene            4905..5642
                     /locus_tag="PC700_001145"
     CDS             4905..5642
                     /locus_tag="PC700_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2580463.1"
                     /translation="MIKVLICDDDKIVRDDIANIVKESGYVDYVKKVKDGIEAIEFIK
                     SEKIDLLIIDIDMPYKNGIDSAKEILSIDKDIHIVFVTGFADYSLESFKVHPIDFIVK
                     PFKKEKILDSINIAIDHINSHKIAKNNFIEDTLFVYKIRKQIHMINFDDIVMFEKNSR
                     AINLYTRDEDTIKFYENFDDLKKRLPPNFFMTHRQYIVNLRLIHKVIPINKSSLEIKF
                     INFQESATLNKSLEKDFLYRFYRVKRF"
     gene            complement(5802..10859)
                     /locus_tag="PC700_001146"
     CDS             complement(5802..10859)
                     /locus_tag="PC700_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HDF2580464.1"
                     /translation="MLERTRVEKNPKIIENFKNNFTKSSSKQYYDLAKSLWDCIENSS
                     YYYREPEDKFLNMIKKYYNCHYYEIKNMDELLSGPLKNALELIFTSEQVEELCQLIKR
                     KMDYTYSGGYYRKGYRSNKLVDYFRGIIRFISNYVDLIYYDFSVIDYLTLPNKVLYYD
                     SIMSDKIALEIDKGNQKVTSALEEVIFGENQTALLTRQMIIGIMKSHSDKCYEMLSKL
                     LLAAKQQEGLRQQIVESLDEGTISASIYMLKTILDNNLERFSSVIRALDTWTGLGFSD
                     QKPGIIRKCLELSYKSLTDEKFRIESINSNDNLEIYFALWATATYNIYDSYSLIENLL
                     SCQEKYKKLVALYFLSHTESTYFKHSIASKNIKGDDLEILAWVITNFYVEGSVGYYYS
                     WQNRETNFDLSNTPIDKDFDARKEQFESLKHTLEILNSKTKSFSGSVFPWTWIEISSH
                     EILKCMMTLTAYDMNAQFIDVLMENCHLMSSDLRASLIVNFVNEPKTDRQREFLLECL
                     SDKSVSNRETVLSLLRKLELTDVELDKITNLLSLKTSSIRQTVIKILLEQDDSNLENI
                     ASSLIKSSNSQKCLGGLEIVNLLKDNSDRQELYNKLILLIEDIDKSKKNTSQVQILID
                     NLLGKAKNDYCKENGFGIYNPENKVQIPEQFDFKDDSNSSSKLNKIISNISLSTSEDS
                     VSVATKKEAKKILDTDINKIIKVYEKFDELITENADYEYEVERWSGNVDKVLLGYSTY
                     NLTPIKYKVSNDTLDNYPLAELWRETSKKLDLDYKLILDILFYSISTCGYNETEKSWF
                     SSIYSNVLDSKKQNKIYTKLEKFKFNGLTRKLLRVLLNEFDHKEIFNYCLEIYYKLRN
                     LLTIEQCKKQRVEFTKNYYRQNEYCPALNAPILNYWLDKAHSFIYDDISFTKFFYTTF
                     SNYQLLEYKFPFYTSINVFARAHELGLMTEEQVNREMISGYNASNIIRTLTNPRWAGD
                     TLEKYPWLKDNLKTVTNRIIEIESRRGDMPTEVSHLANNIQYFEGIDYFVNILVALGK
                     ETFQRGYSYGSNYSKKDVLSSLLKNCYPTKEDDSKKLKSLLKNTDITELRLIESAMYA
                     PQWVNIIEKYLNWKGLKSTIWYFHAHINEGFSAQKESEVALFSPISPRSFIDGAFDVD
                     WFLDSYKTIGEERFNILYKSAKYITSGGNSHRRAQLYADATLGKLDKDMLMKEIETNR
                     NQDKLRSFSLLPMKKGDLKEATFRYEFIQKFAKESKQFGAQRRESESKACTIALENLA
                     RSAGFGDVNRMMWALETEKLNEVAHLFKPKDIDGTSIWIDVDESGKASTKVQKGNKSL
                     KSIPKAIAKHPYVLELKSVQKDLKSQYSRAKQSLEQAMKNEIVFTVKEIANIYKNPVI
                     CPLIKNLVWVCDDNIGYVYNDKSKFFICNIEGETYKLTAKNKLKLAHPVHLFESGKWS
                     EFQHDLFSKQLVQPFKQVFREYYRINSDEISEKTISRRYAGHQIQPKKTVALLRNQLW
                     TVDYEEGLQKVFYKENIVAKMFALADWFSPADIEPPTIETVEFFDRKTYKSLELASIP
                     PILFSEVMRDIDLVVSVAHAGGVDPETSHSTVEMRIAIAKELLLLLKINNINFEGSHV
                     LITGNLGEYSVHMGSGIVHKKGMGALNILPVHSQSRGKIFLPFADEDPKTAEIMSKIL
                     LLAEDNKIKDPSILSNL"
     gene            complement(10884..11303)
                     /locus_tag="PC700_001147"
     CDS             complement(10884..11303)
                     /locus_tag="PC700_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580465.1"
                     /translation="MQIYFRVKAVGKRKPMLELTPFDVSDNINCLKEAISEIVSKNVL
                     EYNEKIPEKSIINFLTNEEIESQADIGKVGFGSIYNENKQSVEKAIEVALQAFEDGIY
                     KVLINEDVIEKLDSPINLKSGDIFTFIRLTLLAGRMF"
     gene            complement(11536..11787)
                     /locus_tag="PC700_001148"
     CDS             complement(11536..11787)
                     /locus_tag="PC700_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580466.1"
                     /translation="MNRYTKIINMMGSYYTKDFEKEKKNVIKVREVKEDTVRKFFLQG
                     DCEVLVVFEDTGKEILIDDFSPEEDIKKYLGTKFINKKR"
     gene            11876..12607
                     /locus_tag="PC700_001149"
     CDS             11876..12607
                     /locus_tag="PC700_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HDF2580467.1"
                     /translation="MTSIKNKDILNCIDYSIKNKLFDKLNDVYKSLPVGNCSGCGNCC
                     MESVGINLIEFLNIFCYLEDRVDLKKSCISKILDYYFEEYTRKNPCPFKDTNNRCLIY
                     EVRPLNCRMFGHWKKEDYNKNLDNVIEKNNNYRELMKKQYGFEINDEVVNYRIDYCDR
                     FIPNKDYLSKSKRLSFFDELMILDSNIYSSGNIDIDFRDRGIVEYFIESLLYRNLAYN
                     VKIRISKEYKIRKRTIDRIKRIVLV"
     gene            12666..13577
                     /locus_tag="PC700_001150"
     CDS             12666..13577
                     /locus_tag="PC700_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2580468.1"
                     /translation="MNLTKVKGNTFFIKGGTNTGIYVFEDNTALMIDPGLCGSRPRRV
                     NKVLDENNIQVRYIINTHGHDDHYGACNQFKEYYKDVCIMSSEYDKLYIENPELFSKY
                     IVGGKSNVFMDNILKNKMLDDIKIDKSIEVGKIELNNEMFDIIEFQGHTPGSIAVLTK
                     DNVIFVGDLLIGDEILSKYDFLFLYDIQEQINSLEKLKNIDFEYLVLGHGKQVISKED
                     SYKLIEKHLNALKKYLYQIRELLVEPKSLEILLKNIINNNNLSNNYKEYHFFKSSLVS
                     VISYLVDLEEIGYILENGELLYYTKTK"
     gene            13628..14440
                     /locus_tag="PC700_001151"
     CDS             13628..14440
                     /locus_tag="PC700_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HDF2580469.1"
                     /translation="MEYEKWNEILAPYEHAVEELKIKFKNIRKEYLAKGEYSPIEFVT
                     GRTKKISSIISKLKRINAKDIETEIDDIAGIRIMCQFVEDIYAIVDLIKVRNDMTIIG
                     EKDYITNYKDSGYRSYHVIIKYPINSIAGSKEIICEIQIRTLAMNFWATIEHSLKYKY
                     DHYIPETLAERLRRASDAAFLLDQEMSEIREDIMKAQAMYQMKSIAIRDTLNRIQELY
                     DLGDTHKAMQYQRKLDRTETDKNITEILELKQEIDILLEQYKDTKVDDKVVQ"
     gene            14550..15242
                     /locus_tag="PC700_001152"
     CDS             14550..15242
                     /locus_tag="PC700_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2580470.1"
                     /translation="MSLYAIGDLHFSTSVNKPMNIFGSNWDGHEKKIIDNWKEVVKEE
                     DMVLVLGDTSWGINLSEAKKDLDIISKLPGQKILIKGNHDYWWTTVTSLNKLYEDMRF
                     IQTNFYEYKDYAICGGRGWICPNDVKFDETDEKVYKREEHRLRLSLESARKSGHSKII
                     VITHYPPTNDKLEESLFTKLFEEYNVEKVIYGHLHGKESFKMGLKGIRNGVEYTLASC
                     DYTEFNLIKVHD"
     gene            15329..16270
                     /locus_tag="PC700_001153"
     CDS             15329..16270
                     /locus_tag="PC700_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580471.1"
                     /translation="MRWILVFIFLYLIPLVVIFRNYKNFKRSCIYSSIYVVLATTIMI
                     SNIYMSGLNKIKESMYYHNYISDETYDEKYVSNFKEKESTDLKQDNKKNEEISNNKNL
                     NNKQDRNIQNKESNEEGSNDIKEKNNSDSNVSVNNKSEKQEDNTKENNKYTSKEKNSE
                     DIKKIDMESIGIFKKEIYNIERAALVPMRECIPYTKDIAKNITKLSSIKKDVTNARNK
                     CKEVVKEYENMQIPELSKEKYTETLSNARDDVKKAYELREKSMESALKLINTKSPKYI
                     GKITEYLDLSDKQIESFKNRLNDLKEEINNSETNNIN"
     gene            16307..17464
                     /locus_tag="PC700_001154"
     CDS             16307..17464
                     /locus_tag="PC700_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDF2580472.1"
                     /translation="MNKQSIGKLNMFFMGITQRNLDNRHFFIELEVIFKSGRKEFKGI
                     AIEEDNKYKFNFKGKSDLYTFNELLKNISKEAENYDGLVFKYVERGTIVVIEGDNKKV
                     NVKYLDNKEEVPKIDEFTASQIKNRDYYVKVGQANALLKEIGVLTKDGKIKNDKIRKY
                     NQIDHFVELIDSILKEIKDKDCITILDCACGKSYLSFVLNFYIKEVLKKNCYFIGIDY
                     SDVVIEASKNMAKNLGYKNMSFIKEDLTNYTPNRDVDLVISLHACDTATDMAIGLGIR
                     AKSEAIVVVPCCHKELLGQYRYEAMEPILKHGVFKARFADLITDGLRTLLLEGNGYDT
                     SVVEYISPLDTPKNLMIRAIKTKTNNDKALKEYKELKSQFGVEPTLEKLIY"
     gene            17616..18176
                     /locus_tag="PC700_001155"
     CDS             17616..18176
                     /locus_tag="PC700_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDF2580473.1"
                     /translation="MNKLNICIDIDGTITSPYHFLPYLNDIFNKNITEAECITHNWEE
                     LYGSGIRDVYAEFNNKYIHSYDEAKIVEGATEVISTLSKGHNLSFVTARHECLTDVTK
                     NWLSRQGFSDIDVYLLGSDYKVEKARELSCDIFIEDNPLNSVQLADDGVKVILLDTNY
                     NKDVKHQNIVRVSNWIDIEKIIKQGI"
     gene            18204..18695
                     /locus_tag="PC700_001156"
     CDS             18204..18695
                     /locus_tag="PC700_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580474.1"
                     /translation="MIDLKIENRKGKIHVKSSEIKDILELRPDFEYVQDISNTINQEN
                     ILVFDCQLTGNSFDMDDLDIEEILEELGEEIDESYFSVLFEDVRAYLKDATDEIEADL
                     QDNYLFDNLRCYFDIYNINQEFTDFKFVFLVSFKDIKISSLANLAKIVSKRQLIGASK
                     FYS"
     gene            18924..19475
                     /locus_tag="PC700_001157"
     CDS             18924..19475
                     /locus_tag="PC700_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2580475.1"
                     /translation="MQSLNEIIAENLKKIRKEKHLSLDKIAQLSGVSKSMLSQIEKCE
                     VNPTISTLKKITNGLKISFTSLMERQESDIELIQKSDIDHFIEDNGKYISYPIFPFDS
                     KRRFEIFMIEIEEGGNLDSNAELPGTQEFITVFSGEVTIKINGEDYIVSSGNSIRFKA
                     DVSHIYRNSGKGIAKMSMVVYYI"
     gene            19561..20493
                     /locus_tag="PC700_001158"
     CDS             19561..20493
                     /locus_tag="PC700_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HDF2580476.1"
                     /translation="MNNKKAVFFFCSDLKKDPVASNVIKCCEEIMDLNQTDIVIDDNF
                     VLEFKDKNNNLFHFVKTKDVISHNYKYYLPILNRHFRDYDFAGVVNWHEGENAPEHIL
                     TAHTIGDVPTGEFGNSNPRYFKNLINAIEDIKNENLLDEFTTLTEATHWSGTTYGEES
                     KLITEYSVPMLDIEIGSSSDSFNNSIAIQVLAKSLIRVFDCDEPLKTLLCVGGVHFEK
                     SFSDIIKNKEYNISIGHVLPNQWIVSGMYDDESGFEKLEKCINSIEGGIDCIVFHDKL
                     KGTYKEQCRKLGEKLNVPVFKHKILKNPKDLPIW"
     gene            20548..21411
                     /locus_tag="PC700_001159"
     CDS             20548..21411
                     /locus_tag="PC700_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDF2580477.1"
                     /translation="MILSYFALLIRVVLLAFERVVVRLLGDEEGDIYKNIASSFLFFF
                     IGGVCLLPFSIMDRVSDWSFLIPCYISSLVYSIGSVAYVTSLATGEVSLVTPINSLNS
                     LFLLLLSVIFLGEGLSLAKVAGIIIMIGGVFILKKVSSFLKNVSVIFNNLPCRLMFLY
                     IFLQSVGRVLDKAFFVQVSPILYSTILYFFVGLNLFIFLAFKKKQKIICEIFSNKKGL
                     SILSGAINGYSYLALLIALNNLELSIAEPFSQVSMIITLILAHFIFKENIKEKIPGSI
                     LILIGGWLLLL"
     gene            21658..23520
                     /locus_tag="PC700_001160"
     CDS             21658..23520
                     /locus_tag="PC700_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2580478.1"
                     /translation="MNYKKTAIDIIEQVGGSKNILALEHCSTRLRFKLIDRSKVNQDA
                     LKKVQGVMSIVNGAQFQVVIGNSVVDVYDEILKVCPLDTNDSTVKTEEKINLGTRFIE
                     FIINIFQPLIFAIAGAGILKSLLMLLAMFNILKSDSTIYTLLVAISDATFYFLPLMVA
                     VTTANVLKCNRLVAIAAVGYLLLPATTTALSEGVELFGFTIPNIAYNAQVFPAILCVS
                     FLATMEKIFNKYSPKPIRTFFVPMMSLAITVPITLTILGPLGYNVGTIFTTIILFLYN
                     KMGFLVVGLLAAFLPFMVATGMHKALIPYSINTIGKLGYEALYMTSSLAHNISESGAC
                     FAVALRTKDETLKQTALSAGISALMGITEPALYGITLQHKRAMLGVVFSSAISGTFLG
                     LFAVKGFVIVGPGLASMSMFIDPNNGNNLIFASIGFVIAILGSFIITLIIWKEDTNTV
                     ESSIGDVVEEELEEEKEVNILVTPVEGKVIDLSKVNDELFASKTLGDGVAIIPTNGNL
                     YAPCDSEVVMLFETKHAIGLRTKNGAEILIHIGINTVSMNGDGFKTFVKTGDNVKEGD
                     LLIQFDLDKISHANLDSTVMIVNNNGADYAYKVLNQSYGNVKKGSILFDVKGGI"
     gene            23523..24989
                     /locus_tag="PC700_001161"
     CDS             23523..24989
                     /locus_tag="PC700_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDF2580479.1"
                     /translation="MRKFPEGFLWGGATAANQFEGGWNLGGKGWSVSDVAKAHFDADV
                     KDYKSNNEITTKDIEEGLAHPEDEVNYPKRHGSDFYHHYKEDIALMAEMGFKTYRMSI
                     AWSRIFPNGDDKEPNEEGLQFYDDVFDELISYGIEPLVTMSHYEPPLNIVLNYDGWYS
                     RQVINMFVRYVETICERYKNKVKYWLTFNEVDSMIRHPYTTGGLVRDRFKDKNFEEVI
                     FQAMHHQFVASALATKICHEIIPNSKVGCMLTKLTYYPYTCRPEDVLATQQKMRSIYA
                     YSDTQVFGEYPVYLLSYFKNNNIQIAKEEHDDEIMKKYPVDFISFSYYMSSCEAADTT
                     GLDITPGNTLLAVKNPYLEMSEWGWQIDSIGLRISLIELYDRYRKPLFIVENGLGAKD
                     ILTEDKKVHDQYRINYLKEHFKCMLDAIIEDGVELWGYTSWGCIDLVSESTKQMSKRY
                     GYIYVDADDYGKGTYNRYKKDSFYWYKKVIENNSIDFK"
     gene            25013..25849
                     /locus_tag="PC700_001162"
     CDS             25013..25849
                     /locus_tag="PC700_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2580480.1"
                     /translation="MNYIIKKVLNSSVVLVHDAKYNEFILLGKGIGYGKKTGEYICGS
                     NDNQMFVPVENTKSKKFLELMDDIPVDILKITQEIIVEAEKLLNSSFNKNLYLILADH
                     FNFAIERMRKGIKITNRVFWEIKNYYPNEFKVGMVAIYMVEERLGIQLPEEEAANIAF
                     HFANAMASDGNSYDTIKYAKVIGEIINIFVFSLNRTLDKKSMHYMRFITHIKFFVERF
                     FSDSMLSSGDDLLFNQMKRSNPKEMLIALKVRDFLEKKYGKKLTNEEIAFLVVHIARV
                     KQ"
BASE COUNT         9616 a         3101 c         4063 g         9313 t
ORIGIN      
        1 atgaaaaaat tataaaaata ttcagaaaat tctattgact aatttattta tcatgatata
       61 ataaatttag aaaagaaaaa aattggggcc tattaaggcc cccttactat tttttgatag
      121 atttatttaa aattactttc tcattttgat tttttatatt tattatagag tcagtataat
      181 ccacttcaaa gttggcaaaa gcagagaata aatctatatc tcttaacaaa tgaattgtca
      241 tacattgatt gttcttattt atccttagaa tcaatgctct tacaaatgga aattgtattc
      301 tagaattaaa taagttagaa ccatcagtat ctatatctaa gctacgataa ttaatatcta
      361 taaatatatc tacatcatca ttttcttctc ttattattct gtagtcaaaa tctaagttta
      421 aatttaaatt atctaaaatc aataacattt aaatcctctt ttctataaat agttttagag
      481 tgttaattta agctctccat ctattataga tataattcta tctgcttttc tagcaatatt
      541 ttcattatgt gttattatta taagtgtttg gttatatttt tttatagaac tttttagtaa
      601 atccatgact tcttccgtag ttttactatc caaatttcct gttggttcat ctgctaggat
      661 tatacttggt tttgcagata aagcgcgtgc aatagcaacc ctttgttgtt gaccaccaga
      721 taattgattt ggaagatgat ttttcctctc actaagaccc aataaattta taatgtcttc
      781 gatgtattct tcatcaactt ttttattgtc taactttagt ggtaattcta tattttcttt
      841 tacatttaat actggaatta ggttatactg ttgaaaaaca aagcctactt tttgtcttct
      901 atattttgct aattttttat ctgaaaggca acatatatct ttaccatcga cttttatagt
      961 tccactactt ggtctctcaa gacctcctac taaatgtaga aatgtgcttt ttccacttcc
     1021 acttgggcca attatcgcaa caaattcacc cttatttatt tttaaatttg catcctttag
     1081 tgcatgtaca gctgtttcct tttttccata aatcttacct aagttaagaa tctctaatat
     1141 aggcataatt aatcctccta ttctgtaatg tttatagctt caactatact aattttattt
     1201 attttcctta atggaatata tgtagctaga atacctacaa ttatgtttgc taaaacaact
     1261 aggatatata ttttgtaatc tattgaaaat cccaatccat atgatacaaa attatacgtg
     1321 taatacattc taaactgtat tataagccct accacaatgg taattacact tgagagtatt
     1381 ccatataata ttccctcata gagtatcata tttttaaatc ctctctcaga gataccaata
     1441 gctcttaaca ttccaaattc gctagttctt gaagttaagt tataacttat gttatttatt
     1501 atattaaaca cactaattat aaacattact gctactattc cataagcata agtcagagcc
     1561 ctagctgtca tcttatcaaa gttttctttt tccattagca tattagctgt tgtagttcca
     1621 ggagattcac ttcctatttc acctaagagt gtatttatct tttttacatt tgcattttta
     1681 ttaatatcta cataaactac attatattta tctacaccag ttagtttttt tagatagtcg
     1741 ttacttgtta ttacatctat accctgatct ccagaatata ggtaattatc tgcaaaagga
     1801 taacttacaa tagctccaac tttaaactca tattctagat attcataatt atcttttagt
     1861 tttatataaa gatcttgatc tatctcacct tttggatatt tgacctttat agtatctcct
     1921 acttttatat ctacagcagg cgtttctgta ccaggagtat aacttagctt gtatttattt
     1981 gttttagaaa cttgtggtac gtataatata gcgaggtttt cttttttcat tctttccaga
     2041 tttatactcc cactcactaa gtatttttct aatgaattta tcatctcatc attgtaacct
     2101 cttaactttt gtttcattgt atattttcca gtattcttat tttttatgaa tagaggaaaa
     2161 tccttataat aaggcgttga attaataata ttgtagtaat ctaatccatt taatttatct
     2221 acttctattc ttgtatctaa gatgctggca gttttgactt tgtcaattcc atttatattt
     2281 tttattttat ctatctgatt atcattaagt ccttttatat ccttgaaaga atcagcttta
     2341 ttagattgat ttagttttaa gaaaaaattt gttagaataa aatctgcatt catatatgtt
     2401 tcttcttgac catttttatt ttgtatatct gaaaaagaat atttataatt ctcctttatg
     2461 atcattatac ctcctagact catggataat ataattataa tgaagccctt tttattccta
     2521 aagatatttt tagcagatat agattttgtt actgatatat ttaaagttaa ttttgatatc
     2581 aaagaattga ccttatttat atttttatca gttccaaagg tttttcttat agagtcaata
     2641 ggagaaattc ttcttatctt taaatatgta aagaaactta taattagtat tgtcagtatt
     2701 atacttccaa ttgataaaag tattatttta tctggaataa caaaaggggt aactgttaca
     2761 ttaccttcaa attgaatatt tcctgcagtc ctattaaata tttgtgcacc accaatacct
     2821 gtgcatatac cgataggtat tgctatcaaa gcaagtatga aaagttcata aaacataaac
     2881 ttgaaaatct tgaagttagt ggatccaata gctcttaaga taccatattc ctgtattctt
     2941 tgatatatcg atataacata gatactatat attacaatac cagaaaatag gctcatagta
     3001 attgcagtgt atttacttga gttatctaaa gtaccatttt ccctagtaga ttctataagc
     3061 attttgtttt ttccaacact attctcatcc aacatagtat tttttactat attatctatg
     3121 cttgtattta tatcaatgcc attattaaat tcaatataag cataagaaaa tttatccaat
     3181 tttgattcat tgagatttaa aaaaatttca catgtccctt tatttttctc aataggtcta
     3241 tcttcaagta ttcctacaac tttaaatgtc tctggttttt ctttttgata taatttaaaa
     3301 gtaatgttct gatttatttc tggttttaaa cccagactat ttaaaatcca tttctctacg
     3361 accacctcat tactagcttt tggaaggtga ccttttataa gtttggattg attagtcaag
     3421 taatttttat ttgcatattc taggtttata gctaatcttt cattaggatc tgtatagcca
     3481 tagtgagatg ttatacctaa atttttaata tcttttctag actttatgta ttctaattga
     3541 tttttattta tatctttaaa gtatacatga tcacttccag tttctctttt taatttatct
     3601 acctctgctt gttgagcact tcttgagagg gtaccaatac caacaataag tgatgtagca
     3661 agaattatac ttagagcaat tgctaaagac cttgctttat aacttattat gtatcttagt
     3721 gctattttta aattatcttt catatttacc acttcctttc ttagctaaat caattatatg
     3781 tttatttaaa atatatatca aagtaagtat cttcaagttt tgagtactta attgtacaaa
     3841 tttatatgtg tatttgcaag tttaattaat gtacatgtaa attttgactt tttacataat
     3901 ttttctaaaa tgatataatc ctcatataaa gtgtagatgg gaggggaatt atggagaata
     3961 attttatatt tcctatagta atactaactt tgtttatatt tagtaatctt ataatgaata
     4021 aatacaggtt ttataaagaa ttagacatat taaaaagagg aatattaacg ttttttgtat
     4081 cgtgttttgt atggatttta ttcatggagg gtattgagta tatacaatta agtttaggat
     4141 ataatttttt acttgaagga gtattaaata ttatactttt tttaatttta attattttaa
     4201 gcttttatat aggatttaaa tttataagat tactagacaa taacaataaa caagagtata
     4261 agctacaaaa attagacagt aaaataaatc ataaggacaa tataatcgta aaattaagaa
     4321 gtcaaaaaca tgactattta aaacatttac aagtagtata ttctctatta aatactgggt
     4381 tagctgaaga tgcaaaagaa tatataacga atatatcaaa tagctttaaa tcaaaaaatt
     4441 caagatatgg gaaagtatct tatttagatg ctatagcttc cttaaaatat gaggaatgtc
     4501 ttgaaaagaa catatctttt gatgtatata tccaagattt tatggataat ttagagattg
     4561 aacctaatga tatgagttca gtgctattaa acataataga caatgcaata gaatctttgt
     4621 ctgctataca aaaggaaaag aagtatataa gattacatgc atatgaagat gaatttcagt
     4681 atattatagc cataaaaaat aatggtccta aaatacataa tgtcgaaagt atatttttgg
     4741 atggatttag cactaaagaa gggcaagaca gaggttttgg gttatatcta gttaaaaata
     4801 tactagataa gtatgattat agtatacttg taaatagtga cgatttttta actgaatttg
     4861 taattttaat accaaaatat tattagttag gaggagagaa gtctgtgata aaagttttga
     4921 tttgtgatga tgataaaatt gtaagagatg atatagcaaa tatagttaaa gagtccggat
     4981 atgtagatta tgttaagaaa gtcaaggatg gtatagaagc aattgaattt ataaaaagtg
     5041 agaaaataga cttattgatt attgacattg atatgccata taaaaatggg attgattctg
     5101 ccaaagaaat tctttctata gataaagata tacatatagt ttttgtaaca ggttttgctg
     5161 actatagtct tgagagcttt aaagtacatc ctatagattt tatagttaag ccgtttaaaa
     5221 aagaaaaaat acttgattca attaatatag caatagacca cattaactct cataaaattg
     5281 caaaaaataa ttttatagaa gatactcttt ttgtatataa aataaggaaa cagatacata
     5341 tgattaattt tgatgatata gtcatgtttg agaaaaattc aagagctatt aatttatata
     5401 ctagagatga agatacgata aaattttatg agaactttga tgatttaaaa aaacgcctac
     5461 ctcctaattt ctttatgact catagacaat acatagtaaa tttaagactt atacataagg
     5521 ttatacctat aaataagtct tctttagaga taaaatttat aaattttcaa gaaagtgcta
     5581 ctttaaataa atctttagaa aaagattttc tatacagatt ttatagggtt aaaaggtttt
     5641 aatgaagaaa aaaattttca gtgatataca atatgtatga tgaagatatc atataaattt
     5701 gctatagtta taaattaatt cgtataaaat ataatataaa acatattgga ctttcatgga
     5761 aaattagaaa gcccaatatg ttttagagat aaatcttata tttataagtt gcttaagata
     5821 ctagggtctt taatcttatt atcttcagca agtaataaga ttttagacat aatctcagca
     5881 gtcttagggt cttcatcagc gaatggaagg aatatcttac ctctactttg agaatgtaca
     5941 ggaagtatat tcaaagcacc catccctttt ttatgtacaa taccagaacc catatgcaca
     6001 gaatactcac ctaaattacc tgtgataaga acatgagagc cttcaaaatt gatattgtta
     6061 atttttaata ataataaaag ttctttagca attgctatac gcatctctac agtagaatgg
     6121 ctagtttcag ggtcaacacc accagcatga gcgacactta caacaaggtc tatatcacgc
     6181 ataacttctg agaatagaat tggaggtata gaagctaatt ctaaactttt ataagttttt
     6241 ctatcaaaaa attctactgt ttcaatagta ggaggctcta tatctgctgg tgaaaaccaa
     6301 tctgcaagtg caaacatctt tgccactata ttttctttat aaaatacttt ctgtaatccc
     6361 tcttcataat cgacagtcca aagctgattt ctaagaagtg ctacagtttt ctttggctga
     6421 atttggtgac cagcatatct gcgagatata gttttttcag atatttcatc agaatttatt
     6481 ctataatact cacggaatac ttgcttaaat ggttgaacaa gttgcttact aaataaatca
     6541 tgttggaact cactccactt accagattca aacaaatgaa ctggatgtgc aagttttaat
     6601 ttattttttg ctgtcaactt ataagtttca ccttctatat tgcaaataaa gaacttagac
     6661 ttatcattat aaacatatcc aatattatca tcacataccc atactagatt tttaatcaaa
     6721 gggcagataa ctggattttt atagatattg gcaatttctt taactgtaaa tactatttca
     6781 tttttcatag cttgttctaa actctgtttt gcacgagaat actgactctt taaatctttt
     6841 tggacagatt taagttcaag tacatatgga tgtttagcaa tagcttttgg tatactcttt
     6901 aaagacttgt tgcctttttg aactttagta gatgcttttc cgctttcatc aacatctatc
     6961 caaattgaag taccatcaat atcttttggt ttaaatagat gagcaacttc atttagtttt
     7021 tcagtctcaa gagcccacat catacgattt acatctccaa atccagcact tctagctaga
     7081 ttttctagcg ctatagtaca agcttttgat tcactttcac gacgttgtgc accaaattgc
     7141 ttgctttctt tagcaaactt ttgaataaat tcataacgga aagttgcttc ttttaggtct
     7201 ccttttttca taggaagtaa agagaaactt ctcaatttat cttgatttct atttgtttca
     7261 atttctttca tcaacatgtc tttatcaagc ttaccaagtg tagcatcagc atagagttgt
     7321 gcacgtctat gagagttacc accactagtt atatatttag ctgacttgta aagtatattg
     7381 aatctttctt ccccaatagt tttatatgaa tctaagaacc aatctacatc aaatgcacca
     7441 tcaatgaaac tacgtggtga aataggggag aatagagcaa cctctgattc tttttgtgca
     7501 gagaagcctt catttatatg tgcatggaag taccaaattg tacttttcag acccttccag
     7561 tttaaatatt tttcaattat gtttacccat tgaggggcat acatagcaga ttctattaat
     7621 cttaactcag taatgtcggt atttttaagt aaacttttta atttttttga atcatcttct
     7681 ttagtaggat agcaattttt aagtagagaa cttaaaacat ctttttttga ataattgcta
     7741 ccatatgaat atcctctctg gaaagtttct ttaccaagag cgaccaaaat atttacaaaa
     7801 taatctatac cttcaaagta ttgtatatta ttagctagat gagacacttc tgttggcata
     7861 tcacctctac gtgattcaat ttctataatt cggtttgtaa cagtttttaa attatcttta
     7921 agccaagggt acttttctaa tgtgtcacca gcccatctag gatttgttaa tgttcttatt
     7981 atatttgatg cgttgtatcc acttatcatt tctctattaa cttgctcttc agtcattaaa
     8041 cctaattcat gtgcacgagc aaacacattt atgctagtat agaaaggaaa cttatattct
     8101 agcaactgat aatttgaaaa cgtagtgtag aaaaacttag taaaagaaat atcatcataa
     8161 ataaacgaat gtgctttatc aagccaatag tttaaaatag gggcatttaa agcagggcag
     8221 tattcatttt gtctgtaata gtttttagta aattcaactc tttgtttttt acattgttct
     8281 atagtcaata agtttctaag tttatagtat atttctaaac aatagttaaa tatttcttta
     8341 tggtcaaact catttagtaa aactcttaaa agttttcttg taagtccatt aaatttaaat
     8401 ttttcaagtt ttgtatatat tttattttgt tttttagagt caaggacatt tgaatatata
     8461 gatgaaaacc aacttttttc tgtttcatta tatccacatg tacttatact ataaaataag
     8521 atatcaagta ttaatttgta atctaagtca agctttttac tagtttctct ccaaagttct
     8581 gccaatggat aattatcaag agtatcatta gaaactttat atttaatagg agtcaagtta
     8641 taagtagaat aacctaaaag cactttgtct acattacctg accatctttc aacttcatat
     8701 tcataatctg cattttcagt aataagctca tcaaattttt cataaacttt tattatttta
     8761 tttatatccg tatcgagaat tttttttgct tctttttttg ttgctacaga aacagaatcc
     8821 tcactagtag ataaacttat atttgaaata attttattaa gctttgatga gctattagaa
     8881 tcatctttaa aatcaaattg ttctggaatt tgaactttgt tttcagggtt atatattcca
     8941 aaaccatttt ctttgcagta atcatttttt gctttaccta acaaattatc aattaatatt
     9001 tgaacttgag atgtattctt tttagattta tcaatatcct ctataagtaa aataagctta
     9061 ttatatagtt cttgtctatc agaattgtct tttaataagt ttacaatttc aagacctcca
     9121 aggcattttt gagaatttga agattttatc agtgaagaag caatattttc caaatttgaa
     9181 tcatcttgtt caagtaaaat ttttattact gtttgtctaa tagagcttgt ctttaaagac
     9241 aatagattgg ttatcttatc taattctaca tctgttagct caagctttct aagtaaactc
     9301 aataccgttt ctctattgga aacactctta tcacttaaac attccaataa aaattcacgt
     9361 tgtctatctg tctttggttc atttacaaaa ttgactataa gtgaagctct taaatctgaa
     9421 ctcataagat gacaattttc cataagcaca tctataaatt gagcattcat atcgtatgct
     9481 gttaaagtca tcatacattt aagtatttca tgagaagaaa tttcaatcca agtccatgga
     9541 aatacacttc ctgaaaatga ttttgtcttt gaatttaaaa tttctaaagt atgttttaaa
     9601 ctttcaaatt gttcttttct agcatcaaag tctttgtcaa taggagtgtt tgacaagtca
     9661 aaattagttt ctctgttttg ccaactatag tagtagccaa cagaaccttc tacatagaaa
     9721 tttgtaatta cccatgctag tatctctaaa tcatcaccct taatgttttt agatgctata
     9781 gaatgtttaa aatatgtact ttctgtatgc gataaaaagt aaagagctac aagtttttta
     9841 tatttttcct gacaagaaag taagttttca ataagagagt aagagtcata tatgttgtaa
     9901 gttgcagtag cccataaggc aaaataaatt tcaagattat cattgctatt aattgattct
     9961 atacggaact tttcatcagt caatgattta tatgaaagtt ctaaacattt acgtattatt
    10021 ccaggttttt ggtctgaaaa accaagtcct gtccatgtat ccaaggctct tataacagaa
    10081 ctaaatcgct ctaaattgtt gtccaaaata gtttttaaca tgtatattga tgcagaaatt
    10141 gtaccctcat ctaaactctc tacaatttgc tgtctaagtc cttcttgttg tttggctgcc
    10201 aaaagaagtt tagaaagcat ttcatagcat ttatcactat gactcttcat gataccaatt
    10261 atcatttgtc ttgtaagtag agcagtttga ttttctccaa atattacttc ttctaaggca
    10321 cttgttactt tttgatttcc tttgtctatt tctagtgcta ttttatcact cattatagag
    10381 tcatagtaca agaccttatt tggaagagta agataatcta taactgaaaa atcataataa
    10441 attaaatcaa catagtttga aataaacctt ataatacctc taaaataatc cacaagttta
    10501 tttgagcgat accctttacg ataatatcct cctgaatatg tataatccat ctttctttta
    10561 attaattggc aaagttcttc tacttgttca gaagtaaata ttaattctaa tgcatttttt
    10621 aaaggtccag aaaggagctc gtccatattt ttaatttcgt agtagtggca attgtagtat
    10681 ttctttatca tatttaagaa tttgtcctca ggttctctat aatagtatga actattttct
    10741 atgcagtccc aaagagattt tgctaagtcg tagtactgtt ttgaagaaga tttagtaaaa
    10801 ttatttttaa aattttcaat tattttagga tttttttcta ctctagtacg ttctaacatt
    10861 tatgtacctc caaattattt attttaaaac attcttccag caagcaaggt aagtcttata
    10921 aaagtaaata tatctccact tttcagattt ataggggaat cgagtttttc aataacatct
    10981 tcatttataa gcactttgta gattccatct tcaaatgcct gtaacgctac ttctattgcc
    11041 ttttctacag attgtttatt ctcattgtaa atagagccaa atccaacttt tccaatatca
    11101 gcttgagatt caatttcttc attagttaag aagtttatta ttgatttttc tgggattttc
    11161 tcattatatt caagaacatt tttagataca atttcagaga tagcttcttt taggcaattg
    11221 atgttgtcag atacatcaaa aggggtaagt tctaacatag gtttgcgttt gccaacagcc
    11281 ttaactctaa aataaatttg cataaaatca cctcgcaaaa tattaattat gtatttagaa
    11341 atttaaaatt tatctatttg ttatattata tcattttgtg aattattaat gtgaattatg
    11401 gaattatttt acaaaaaata gaaaaaaagc tatcttaaag tctaaaaaag caataaaaga
    11461 taacttttaa ctataattat atagaattat aactgtacat ttattaaatt ttaattttag
    11521 aattattttt gataactatc tttttttatt tataaatttt gttcctaaat attttttgat
    11581 atcttcttca ggagaaaagt catcaataag tatttctttc ccagtgtctt caaatactac
    11641 aagaacttca cagtcacctt gcaagaaaaa ttttctaact gtatcttctt taacttctct
    11701 aacttttatt acattctttt tttctttttc aaagtctttt gtgtaataac ttcccatcat
    11761 attaattatt tttgtatatc tattcataaa atacctccat atgcgattaa atttaaaaaa
    11821 tatgttataa taattgtttg tattaactta ataattataa caaggagtaa taataatgac
    11881 tagtataaaa aataaagata ttttaaattg tatagattat tccattaaaa ataagctctt
    11941 tgacaaacta aatgatgtat ataagtcctt gccagtagga aattgttctg gatgtggaaa
    12001 ttgttgtatg gagtcagtag gtataaattt aattgagttt ttgaatatat tttgttatct
    12061 tgaagataga gttgatttaa aaaaaagctg tataagtaaa atattagatt attattttga
    12121 ggagtataca agaaagaacc catgtccatt taaagatact aataatagat gtctaatata
    12181 tgaagttaga ccattaaatt gtaggatgtt tggacattgg aaaaaagagg attataataa
    12241 aaatttagat aatgttattg agaaaaataa taactataga gagttaatga aaaagcaata
    12301 tggatttgaa ataaatgatg aagtagtaaa ttacaggata gattattgtg atagatttat
    12361 tccaaataaa gattatttaa gtaaatctaa gagattaagc ttttttgatg agcttatgat
    12421 attagactca aatatttatt caagtggaaa tatagatata gactttaggg atagaggtat
    12481 agttgaatat tttatagagt cattacttta tagaaattta gcatataatg tgaaaataag
    12541 gatatctaaa gaatacaaaa taagaaaaag aactatagat agaataaaaa gaattgttct
    12601 tgtatagttt tatattagta agtaataaat tgtatcataa atattgtcaa aatgaggtga
    12661 ggaaggtgaa tcttacaaaa gtaaaaggaa ataccttttt tataaaaggt ggcacgaata
    12721 caggaatata tgtatttgaa gataacacag cacttatgat agacccaggg ttatgtggtt
    12781 caagacctag gagagtaaat aaagtattag atgaaaataa tatacaggtt agatatatta
    12841 taaatactca tggtcatgat gaccactatg gagcttgtaa tcaatttaaa gaatattata
    12901 aagatgtgtg tataatgtca tctgaatacg acaaattata tatagaaaat ccagaattgt
    12961 tttcaaagta tattgttggg ggaaagagca atgtatttat ggataatatc ttaaaaaata
    13021 aaatgttgga tgatataaag atagataaat ctatagaagt tggaaaaatt gaattaaaca
    13081 atgaaatgtt tgatataatt gagtttcagg gtcatacacc tggtagtata gctgttttga
    13141 cgaaggataa tgtgattttt gttggcgatt tattgatagg agatgaaatt ctatcaaaat
    13201 acgatttctt gtttctatat gatatacaag aacaaataaa ttcattagaa aaattaaaaa
    13261 atatagattt tgagtactta gttttagggc atggcaagca agttatatcg aaagaagatt
    13321 cttataaatt aatagaaaaa catttgaatg ctttaaaaaa atatttatat caaataagag
    13381 agttgcttgt agaaccgaaa tctttagaaa ttttattaaa aaacattata aataacaata
    13441 atttaagcaa taattataaa gagtatcatt tttttaaatc ttcgttagta tctgtcataa
    13501 gttacttagt tgatttagaa gaaatagggt atatattgga aaatggagaa ttgctttact
    13561 atacaaaaac aaaataaatt atgataaaat aaagaaggtc ataaaaaaca aataaagggt
    13621 gagcaatatg gaatacgaaa aatggaatga gatactagca ccttatgaac atgcagtaga
    13681 ggaattgaaa ataaaattta aaaatataag aaaagaatat ttagcaaaag gtgaatattc
    13741 tcccatagag tttgtcacag gcagaacaaa aaaaatatct tccataatat ctaagttaaa
    13801 aaggataaat gcaaaagata tagaaactga aattgatgat attgctggta taagaataat
    13861 gtgccagttt gttgaggata tatatgctat agttgatttg ataaaagtaa gaaatgatat
    13921 gactataata ggagaaaaag attatattac aaattacaaa gatagtggtt atagaagcta
    13981 tcatgttata ataaaatacc ctataaactc cattgcgggt tcaaaggaaa ttatatgtga
    14041 aatacaaata agaactcttg caatgaattt ctgggctaca atagaacatt cattaaaata
    14101 taaatatgac cattacatac ctgagactct tgctgaaagg cttagaagag catctgatgc
    14161 tgcctttctt ttagaccaag aaatgagtga aataagagag gacataatga aagctcaggc
    14221 tatgtaccaa atgaagtcta tagctattag agatactcta aatagaatac aagagttata
    14281 tgacttgggt gatacacata aagctatgca gtaccaaaga aaacttgata gaactgaaac
    14341 agataaaaat ataactgaaa ttttggaatt aaaacaagaa atagatatat tactagagca
    14401 atataaggat actaaggtag atgataaagt agttcagtaa aaaactactt gtagaaaatt
    14461 tgctaaatat taatggggct gcctcttaat ggaaaataat ttctaaaaag agacagccta
    14521 tttagtgtaa ttaagaaagg aatgatatta tgagtttgta tgctataggt gatttacatt
    14581 tttcaacatc agtcaataag ccaatgaata tctttggaag taattgggat ggtcatgaaa
    14641 agaaaattat agataattgg aaagaagttg ttaaggaaga agatatggtc ttagtactag
    14701 gagatacatc ttggggaatt aatttatcgg aagctaaaaa agatttagat ataataagca
    14761 aacttccagg tcagaaaata cttataaaag gaaatcatga ttactggtgg acaaccgtta
    14821 caagtttaaa taagctatat gaagacatgc gttttattca gacaaatttt tatgaatata
    14881 aagattatgc aatttgtgga ggtaggggct ggatatgtcc taatgatgtt aaatttgatg
    14941 agactgatga aaaagtttat aaaagagaag agcatagact tagactatca ttagaatctg
    15001 ctcgtaaaag tggtcactca aaaataattg tgattactca ttatcctcct acgaatgata
    15061 aattggaaga atctttattt acaaagttat ttgaagaata taatgttgaa aaggtgattt
    15121 atggtcactt gcatggtaaa gaatctttta aaatgggatt gaaggggatt cgaaatggag
    15181 tagaatatac attagcatct tgtgactata cagaatttaa tttaataaaa gttcacgatt
    15241 aaaaaacata aattggtaaa tactataaga tacatgatta aaaataaata tttgagggat
    15301 ttcggttttg agaaaggaga cacgacttat gaggtggata ttagttttta tatttttata
    15361 tcttattcct ttagtagtga tattcagaaa ttacaagaat tttaagaggt cttgtatcta
    15421 cagtagtatt tatgtggtat tagctacaac aataatgata agtaatattt atatgagtgg
    15481 acttaataaa ataaaagagt ctatgtatta tcacaattat atttctgatg agacatatga
    15541 tgaaaagtat gtatcaaatt ttaaggaaaa agaaagtacc gatttaaaac aggataataa
    15601 aaagaatgaa gaaatatcaa ataataaaaa tttaaacaac aaacaagata gaaatataca
    15661 aaataaagaa agtaatgaag aaggaagtaa tgatataaaa gaaaagaata attctgatag
    15721 taatgtttca gtgaataata aatcagaaaa acaagaagat aatacaaaag aaaataataa
    15781 atatacaagt aaagaaaaaa atagtgaaga tataaaaaaa atagatatgg aaagtatagg
    15841 aatttttaag aaagaaattt ataatataga aagagcagct ttagttccaa tgagagaatg
    15901 tataccttac actaaggata tagcaaaaaa tataactaaa ttatctagta taaaaaaaga
    15961 tgttacaaat gccagaaaca agtgtaaaga agttgtaaaa gaatatgaaa atatgcagat
    16021 tccagaactt tctaaggaga agtatacaga aacattgagt aatgctagag atgacgtaaa
    16081 gaaagcatat gagcttagag aaaaatctat ggaaagtgct ttgaaattaa taaatactaa
    16141 aagccctaaa tatataggga agataacaga atatcttgat ttatcagata agcagataga
    16201 aagttttaaa aatagattga acgatttaaa agaagaaata aataattcag aaacaaataa
    16261 tattaattaa taagatttaa agatataagt taaaagggag attatgatga ataaacagag
    16321 tataggaaaa ctaaatatgt tttttatggg gattactcaa agaaaccttg ataataggca
    16381 tttttttatt gagttagagg tgatttttaa atctggaaga aaagagttta aaggaattgc
    16441 aatagaggaa gataataaat ataaatttaa ttttaaagga aaatcagatt tatatacgtt
    16501 taatgagttg cttaagaata tttctaaaga ggcagaaaat tatgatggat tagtatttaa
    16561 atatgttgag cgaggaacta ttgttgtaat tgaaggagac aataagaagg taaatgtaaa
    16621 atatttagat aataaagaag aagtacctaa aatagatgaa tttacggcat ctcaaataaa
    16681 aaacagagat tattatgtaa aagtaggaca agcaaatgca cttttaaaag aaattggagt
    16741 acttacaaaa gatggtaaga taaaaaatga taaaataaga aaatacaatc agatagacca
    16801 ttttgttgaa ttgatagata gtattttaaa agaaataaag gataaagact gtataactat
    16861 cttggattgt gcttgtggaa aatcttactt aagctttgtc ttaaattttt atataaaaga
    16921 agtattgaag aaaaactgtt attttatagg aatagattat tcagatgtag ttatagaagc
    16981 atcaaaaaat atggctaaaa acttgggtta taaaaatatg tcatttataa aagaggattt
    17041 gactaattat acacctaata gggatgttga ccttgtaata agtctacatg cttgtgatac
    17101 agctacagat atggcaatag gtctaggaat tagagcaaaa tctgaagcta tagtagtagt
    17161 accatgttgt cataaggagt tattagggca atatagatat gaagctatgg aaccaatatt
    17221 aaaacatggt gtatttaaag ctagatttgc agatttgata actgatggtt taagaacatt
    17281 gttattggag ggcaatggat atgatacttc tgtagtagaa tacatatctc cacttgatac
    17341 accaaaaaat ttaatgataa gagcgataaa aactaaaacg aataatgaca aagcattaaa
    17401 agaatataaa gaactgaaat cacaatttgg agtggaacca accttagaaa aacttatata
    17461 ttagttaaaa atgtaaaatt tacgaaaagc ttgcttaaat atgcaagctt tttggtatta
    17521 tataggtata gtcatatcca gagttttgat ttataattaa agctgtgcta taatatttga
    17581 ttaatcaaat caataaatgt tacatggagg taaaaatgaa caaattgaat atatgtattg
    17641 atatagatgg aacaataaca agcccttatc attttcttcc atacctaaat gatatcttta
    17701 ataaaaatat aactgaagca gagtgtataa cacataattg ggaagaattg tatggttcgg
    17761 gaataagaga tgtgtatgcg gagtttaata ataagtatat acactcatat gatgaagcaa
    17821 agattgtaga gggtgctaca gaggtaatta gcacattatc taaaggacat aatttatctt
    17881 ttgtaacggc tagacatgag tgtttaactg atgtaactaa aaattggcta tcgagacaag
    17941 gattttcaga tatagatgtt tacttattag gaagtgatta taaagttgaa aaggcaagag
    18001 aactgagttg tgacatattt atagaagata atcctttaaa ctcagtgcaa ctagctgatg
    18061 atggtgttaa agtaatactt ctggatacta actataataa agatgttaaa catcaaaata
    18121 tagttagagt tagtaattgg atagatattg aaaagattat aaaacaaggt atttaaagat
    18181 ttgtagattg aaagggggag catatgatag atttaaaaat tgaaaataga aaaggaaaaa
    18241 tccatgtaaa gagcagtgaa ataaaagaca ttttagaatt acgtccagac ttcgaatatg
    18301 tacaagatat atcaaatact ataaatcaag aaaatatatt ggtatttgat tgccagttga
    18361 caggaaattc ttttgatatg gatgatttag atatagaaga aatattggaa gagttaggtg
    18421 aagaaataga tgagtcttat ttcagtgttt tatttgaaga tgtaagagcg tatttaaaag
    18481 atgctactga tgagatagaa gcagacttac aagataatta cttatttgat aatttgagat
    18541 gttattttga tatatacaat ataaatcaag aatttacaga ttttaagttt gtatttttag
    18601 tttcttttaa agatataaaa atttcttcat tagcaaacct agctaagatt gtttctaaga
    18661 gacagcttat aggtgcatct aagttttata gttaaaatga aatattatta taataaaagc
    18721 tagtagcctt gtaaaaatat aggttactag ttttttattt gttcactttc acaaaatttc
    18781 agaaaactat ttacattcta aaaaaagtaa tgtatattaa acataatgta cagtataatg
    18841 gataaaaagt ttctattaaa ttactaagtt tatttaataa tcaaaataat ttatcttata
    18901 ttagtatgta ttagggggta tttatgcaaa gtttaaatga aataatcgct gaaaatttaa
    18961 aaaagataag gaaagaaaaa catcttagtt tagacaagat tgcacaactt agtggtgtaa
    19021 gtaagagtat gctatctcaa attgaaaagt gtgaggtaaa tcctactata tctactttga
    19081 agaaaataac taatggacta aaaatctcat ttacatcatt gatggagagg caggaaagtg
    19141 acatagaatt gattcaaaaa agtgatatag accattttat tgaagataat ggaaagtata
    19201 taagttatcc aatatttcca tttgactcca aaagaagatt tgagatattt atgattgaaa
    19261 tagaagaagg tggaaattta gattcaaatg ctgaacttcc aggaacacaa gagtttataa
    19321 ctgtttttag tggcgaagta accattaaaa taaatggaga agattacatt gtatctagtg
    19381 gaaattctat aagatttaaa gctgatgtat cacatatata tagaaactca ggcaaaggta
    19441 tagctaaaat gagtatggta gtatattata tataaataat ttaatggaat tagagtgata
    19501 tttagatgat tttaggtatg aagatatatt atatttaagt tataggagtg tgatttatgg
    19561 atgaataata aaaaagcagt ttttttcttt tgtagtgatt taaagaaaga cccagtagcc
    19621 agtaatgtga taaagtgttg tgaagaaata atggatttaa atcaaactga tattgttatt
    19681 gatgataatt ttgtattaga atttaaggat aaaaacaata acttatttca ttttgtaaaa
    19741 acaaaagatg taataagtca taattataaa tattatcttc caattttaaa tagacatttc
    19801 agagattatg attttgcagg agttgtaaat tggcatgagg gagaaaatgc tccagaacat
    19861 atcctaacag ctcatacaat tggagatgtt ccaacaggag aatttggaaa ttcaaatcca
    19921 agatacttta aaaatctaat caatgctatt gaggatataa aaaatgaaaa tctacttgat
    19981 gaatttacta cattaacaga agcaactcac tggtctggaa caacatatgg tgaagaatct
    20041 aaattaatta cagaatattc tgtacctatg ttggatattg agataggaag tagtagtgat
    20101 agttttaata atagtatagc aatacaggta ttggcaaaat cattaattag agtatttgat
    20161 tgtgatgaac ctttgaagac tttattatgt gttggagggg tacattttga gaaatctttc
    20221 tcagatatta taaaaaataa agaatataac atatcaattg gacatgtatt gccaaatcaa
    20281 tggattgtaa gtggaatgta tgatgatgaa tcgggatttg agaaactaga aaagtgtata
    20341 aatagtattg aaggtggaat tgattgtata gtttttcatg ataaattaaa aggtacctat
    20401 aaagagcaat gccgtaaact aggtgaaaaa cttaatgtac ctgtatttaa acataaaata
    20461 ttaaaaaatc ccaaagactt acctatatgg tagtcttata cagttaatat aacaataagt
    20521 cggattaatt tttaagcgag gtcaaaaatg atattatctt attttgcatt attaattaga
    20581 gtagtgctat tagcttttga aagagtagtt gtaagattat taggtgatga agaaggagat
    20641 atttataaaa atatagcttc atcatttcta tttttcttta tagggggagt atgtttactt
    20701 ccatttagca taatggatag agttagtgat tggtcgtttt taataccatg ttatataagt
    20761 agtttagtct attcaattgg ttcagtagcg tatgtaacgt ctttagcaac tggggaagtt
    20821 tcacttgtta ctccaataaa tagtttaaat tcattgtttt tactgctttt atcagttata
    20881 tttttaggtg aaggtttaag tttagcaaaa gttgcaggaa tcataattat gattggtgga
    20941 gtgtttatat taaaaaaagt aagctctttt ttaaagaatg tctctgtaat ttttaacaat
    21001 ttaccatgta gattaatgtt tttatacata tttttgcagt cagtaggaag agtattggat
    21061 aaggcttttt ttgtacaagt atctcctata ttatattcaa ctatactgta ttttttcgta
    21121 ggtttaaatt tatttatctt tttagcattt aaaaagaaac aaaaaataat ttgtgagatt
    21181 tttagtaata aaaaggggct ttcaatttta agtggtgcta ttaatggata ttcttattta
    21241 gcattattaa ttgctttaaa taatctagaa cttagtatag cagagccatt ttctcaagta
    21301 tctatgataa taactttaat acttgctcat tttatattca aagaaaatat aaaagaaaaa
    21361 ataccaggtt caattttgat actaattggt gggtggctac tgttacttta attgacataa
    21421 aaaatgaatt ataaaggtgc tattagttag tatcagaaaa tttaaaaaag tattgacaat
    21481 tcttttaggc aaatgttaat ataataatac aaaatagtat aaatagaaga catgttactg
    21541 gtaatgcagg caggatcgaa tatattgctt tgatttttca aggtgtttat ataggttctg
    21601 ccttttttgc atcttgaatg gccattcata aaaaattatg gagaaggaga ttacaagatg
    21661 aattataaaa aaactgccat tgacattatt gaacaagttg gtggttcaaa aaatatttta
    21721 gctttagaac actgttcaac tagactacgc tttaagttga ttgatagaag taaagtcaat
    21781 caggatgctt taaaaaaagt acaaggtgtt atgagtatag ttaatggggc acaatttcaa
    21841 gttgtcattg gaaatagtgt tgttgatgtg tatgatgaaa ttttaaaagt ttgcccttta
    21901 gatacaaatg atagtactgt aaaaactgaa gaaaaaataa acttaggaac aagatttata
    21961 gaatttatta ttaatatttt tcaaccactg atttttgcta ttgcaggagc tggtatttta
    22021 aaatcactgt tgatgttatt agcaatgttt aatattttaa aatcagattc tactatatat
    22081 acattattag ttgctatttc agatgcaaca ttctatttcc tacctcttat ggtagctgta
    22141 actactgcca atgtattgaa atgtaatcgt ttagtagcta ttgccgctgt aggatactta
    22201 ttgcttccag caacaacaac tgctttaagt gaaggtgtcg aactatttgg atttacaatt
    22261 ccaaacattg cctataatgc acaagttttc ccggctatct tatgtgtgtc ttttttggca
    22321 acaatggaga aaatttttaa caagtattca ccaaaaccaa ttcgaacttt ctttgttcct
    22381 atgatgtctt tggcaattac tgttccaatt actctaacta ttttaggtcc tttaggatat
    22441 aacgttggta caatttttac aaccattatt ttattcttat ataacaaaat gggattctta
    22501 gttgtaggat tacttgctgc ttttcttcca tttatggttg caacaggaat gcacaaagca
    22561 ctaatacctt attcaattaa tacaattgga aagttgggtt atgaagcttt atatatgaca
    22621 tcttcattag cccacaatat ttctgaaagt ggtgcttgtt ttgcagtagc cttgcgaaca
    22681 aaagatgaaa cattaaaaca aacagcatta tctgcaggaa tttctgcctt gatgggaatt
    22741 acagagccag ctttatatgg tattacttta caacataaaa gagcaatgtt aggtgttgta
    22801 ttctcaagtg caatttctgg tacattttta ggattatttg cagtaaaagg atttgtaatt
    22861 gttggaccag ggcttgccag tatgagtatg tttattgacc caaacaatgg aaacaatttg
    22921 atttttgcga gtattggttt tgttattgca atattaggct cgtttattat tacactgatt
    22981 atttggaagg aagatacaaa tacagtagag tcatcaattg gagatgttgt tgaagaagag
    23041 ctagaagaag aaaaagaagt aaatatctta gtaactccag tagaaggaaa agtaatagat
    23101 ttaagtaagg ttaatgatga actatttgca agtaagactt tgggagatgg agttgcaatt
    23161 ataccgacca atggaaattt atacgcacct tgtgattcag aagttgtgat gttatttgaa
    23221 acaaaacatg caattggttt aagaacaaaa aatggggctg agattttgat tcatattgga
    23281 ataaatactg taagtatgaa tggagatgga tttaaaacat ttgttaagac aggcgataat
    23341 gtaaaagaag gagatttatt aattcaattt gatttagaca aaatctcaca tgctaattta
    23401 gattcaactg ttatgattgt aaacaataat ggagcagatt atgcttataa agtgttaaac
    23461 cagtcttatg gcaatgtaaa aaaaggaagt atcttgtttg atgtaaaggg aggaatataa
    23521 atatgagaaa gtttccagaa ggatttcttt ggggaggagc tacagctgct aatcaatttg
    23581 aaggtggttg gaatttaggt ggtaaagggt ggtctgtatc agatgtagca aaggcacact
    23641 ttgatgcaga tgtgaaagac tataaatcta ataatgaaat cacaacaaaa gatatagaag
    23701 aaggattagc acatcctgaa gatgaagtaa attatccaaa acgtcatggt agtgattttt
    23761 atcatcacta taaagaagat attgctctaa tggcagaaat gggatttaag acatatcgaa
    23821 tgagtattgc ttggtctcgt atttttccaa atggagatga taaagagcca aatgaagaag
    23881 gtttacagtt ttatgatgat gtatttgatg agttgataag ttatggaatt gaaccacttg
    23941 ttactatgtc acattatgaa ccaccattaa atatcgtttt aaactatgat ggatggtatt
    24001 cacgacaagt tatcaatatg tttgttaggt atgtagaaac tatctgtgaa agatataaaa
    24061 acaaagtgaa atattggctt acatttaatg aagttgattc tatgattcgt catccgtaca
    24121 ctactggtgg tttagttcga gaccgtttta aggataaaaa ctttgaggaa gttatttttc
    24181 aagctatgca tcatcaattt gtagctagtg cccttgcaac aaaaatttgc catgagatta
    24241 ttccaaattc aaaagtaggg tgtatgttaa caaaattaac gtattatcca tatacatgta
    24301 gaccagaaga tgttttggca actcaacaaa aaatgagaag tatttatgca tatagtgata
    24361 cgcaggtttt tggtgagtat ccagtatatt tactttcgta ttttaaaaat aataacattc
    24421 aaatagcaaa agaagaacat gatgatgaaa ttatgaaaaa gtatccagta gattttattt
    24481 cattctctta ctatatgtct tcttgtgagg cagctgatac aactggatta gatattacac
    24541 ctgggaatac gttattagct gtaaaaaatc cttatttaga aatgagtgaa tggggttggc
    24601 aaattgattc aataggtcta cgtatatctt tgattgaatt atatgataga tacagaaagc
    24661 ctttatttat agttgaaaat ggattgggag ctaaagatat tttaactgaa gataaaaaag
    24721 ttcatgacca atatagaatt aattatttaa aagaacactt taaatgtatg ttagatgcga
    24781 ttatcgaaga tggcgttgaa ctttggggat acacttcatg gggttgtatt gatttggtca
    24841 gtgaatcaac gaaacaaatg tcaaaacgtt atggatatat ttatgtggat gctgatgatt
    24901 atggaaaggg gacatataat cgatataaga aagatagttt ttattggtat aaaaaagtaa
    24961 ttgaaaataa tagtatagat tttaaatgac caatagaagg tgagcaaata aaatgaacta
    25021 cataataaag aaagttttga actcgagcgt tgttttagtt catgatgcaa agtataatga
    25081 atttattttg ctagggaaag gaattggata tggtaaaaaa acaggagaat atatttgtgg
    25141 ttccaatgat aatcaaatgt ttgtccctgt tgaaaataca aaatcaaaaa aatttttaga
    25201 attaatggat gatattcctg tcgacatttt aaagataaca caggaaataa tagtagaggc
    25261 agaaaagtta ttgaactcat cttttaataa aaacttatac ttaatattag cagaccattt
    25321 taactttgca attgaaagga tgcgtaaagg aataaaaatt acaaatcgag tattttggga
    25381 aataaaaaat tattatccaa atgagtttaa ggttggaatg gttgcaattt atatggtaga
    25441 ggagagattg ggcattcaat taccagagga agaagcagct aacattgctt tccattttgc
    25501 aaatgctatg gcttcagatg gaaattcata tgatacgatt aagtatgcca aagtaattgg
    25561 agaaataatt aatatttttg ttttttcttt gaatagaacc ttagataaaa agagtatgca
    25621 ttatatgaga tttataacac atataaaatt ttttgtggaa agattttttt cagatagtat
    25681 gttaagcagt ggtgatgatt tattatttaa tcaaatgaaa aggtcaaatc caaaagaaat
    25741 gttaattgca ttaaaagtta gagatttttt agaaaagaaa tatggaaaaa agttaacaaa
    25801 tgaggaaata gctttcttgg ttgtccatat cgcacgagtt aagcaataga gtgaattact
    25861 aatattatct tatacgtcta catggtagaa aattgaaaga aggtaaagta tataaagaaa
    25921 ctgagacata ttattttgtt gttcattatg tttattttat gagtgtatcc tgttaaaaat
    25981 ttaagttaat atttataatt aaaatataca aatagttaaa ataagtttat gtggttatga
    26041 atttatttta actatttttt tgtttaatgg aaaatatgtt ttataactag aat
//
LOCUS       DAMJWP010000032        26088 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.033, whole genome shotgun sequence.
ACCESSION   DAMJWP010000032 DAMJWP010000000
VERSION     DAMJWP010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26088
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.033"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            179..1627
                     /locus_tag="PC700_001163"
     CDS             179..1627
                     /locus_tag="PC700_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDF2580481.1"
                     /translation="MKLGKDFLWGGAISANQSEGFYLDGGKGLSQFDKLPLNDKRLKD
                     VCLDEDNILNENNQYYPSHIGIKFCENYKEDIAMLAEIGFKCFRFSISWSRIFPTGEE
                     SEPNEKGLQLYDNIIKELKKYNIEPLITISHFDIPYSLVEKYSGWADRRVVNLYVKYA
                     EILMKRYKDDVKYWIPFNEMNMIIHIPYIGAGLTFIADENQLQKKYQAAHHQLLANAK
                     TIEIGRYINKEFKFGCMLAAGKTYPYTCKPEDVFATLEHERHNLFFSDVQVRGVYPSF
                     MNYYLKSNQIKLIIKEEDLSTLKDNTVDFVSFSYYSSACSSARAEGLEKSKANGPETI
                     KNPYLPKSNSVWQNDPVGLRITMNQLYERYQLPLFIVENGLGTQDIVNEDGKIIDDYR
                     IDYMKEHIKNMILGVNEDGIELIGYLMWGCIDVTSVSEGKMTKRYGIIHVDSDDYGRG
                     SFKRTKKQSAFWYKKVIETDGEILFNDMETHA"
     gene            1653..4043
                     /locus_tag="PC700_001164"
     CDS             1653..4043
                     /locus_tag="PC700_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDF2580482.1"
                     /translation="MSLIFTTKDQLIEQGFSKDTIKSNETLFTLANGHLGVRGNIEES
                     DFNNEYVDNMGTYVNGFYEESPITYGESAYGYAKQNQTICKLPNAHIVNFSIEGDCFD
                     LSKGITSEHTRILDLNEGIVKRSFIWENSRGRKVKVSIERLVSQDIEELMMISYTITP
                     LNFNGKIKFKNKMENSMEKFNCEKVDDPRVASINKKQFKINVKKYNNKYFMNLVTERS
                     RLGLWCGFDSYITCGNITNETYNVSDECIEREIEVYAKKDERLNLEIVVGYSRVYSDL
                     NLNEERLKSLECLESLKCLESEAHLKSLELVLREACNIGFDKVKNIQRKKMNEFWTTS
                     DVEIEGDKTLQLGIKTNLFHIYQSAGRDGKTNISAKGLTGDGYEGHYFWDTEMYILPF
                     FIYTQPSIARALLDYRYSILDASRERARELGINKGCLYSWRTINGNECSAYYPAGTAQ
                     FHINADIAYGIRTYFEATNDEEFMMQNGLEILIETARFWVEFGDYIELKNNKFCINGV
                     TGPDEYTAIVNNNYYTNLMAKYNLIYAVQMVDKFKHNLFSNEVFRKVKLQEYEVKDWI
                     NAAQNIYLPYDEEKKISKQDDSFLEKSVWDFKNTPKEKYPLLLNYHPLTIYRYQVNKQ
                     ADTVLSALLFPDEFSLEQKKRDFEYYEKITTHDSSLSRSIFGMMASEIGNHEKAYNYF
                     MDTALMDINNLQKNTSDGIHAANMGGTWMSLVYGFAGMKIKKDKLSFEPRIPKHWKNI
                     KFNILFRDNLVSVNIEEYKSYYKLIKGKNISILHDNKEIKLSKNIIEVNNCFKGSN"
     gene            4045..4716
                     /gene="pgmB"
                     /locus_tag="PC700_001165"
     CDS             4045..4716
                     /gene="pgmB"
                     /locus_tag="PC700_001165"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDF2580483.1"
                     /translation="MKAVLFDLDGVITDTAKYHFEAWHEIAKSLDIEIDETFNEKLKG
                     ISREESLDKILLSANKVDSYTKEEKLKVCEEKNNIYIKLINSLTYKDILPGINELIKE
                     LKKNNVKLGIASASKNAPRILESLGIKDEFDCIVNPSLLERGKPYPDIFIEGSKMLNL
                     KQYDCVGIEDSYSGIKAINAANMTSIGVGKKEVLGKCDHIVEDTSLLNYNTLLQAWSK
                     KINLD"
     gene            4755..6155
                     /locus_tag="PC700_001166"
     CDS             4755..6155
                     /locus_tag="PC700_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2580484.1"
                     /translation="MKNKYEEVAQLIIDGLGGKDNIIDLTHCMTRLRFILKDEKKVNA
                     DKLKSIDKVVGVNSTSTQYQVIIGNEVGVVFNAIINKGINTTGDIKSDIKNENKKNSI
                     LNNIIDTITGCMTPMIPALTASGMIKVILSLCTTFNWLSSNSSTYRVLDIIGDVAFYF
                     MPIILAINAAKKFNVNTSIAVIVVGVFLHPNFSAWVSSGDPISFIGVPIQGVIYAASV
                     IPALLTVWMMSYIEKFINKLTPSMLKTILNPTLVLLISAPIALIVIGPIGNFLGEGLA
                     SIINLLQGRLGFIMVCLLAAAMPFIVMTGMHHALTPIFISAFATTGQESLILISQVCA
                     NLAQGGATLAVALRSKDSKMKQLASAAWISAIMGITEPALYGVTLKLKKPAMLASIGA
                     GIAGLFAGITHVTLYVPQNSIMAVLGLSGEKGISNIVNGLIVIFIALVAPFIMTYVFG
                     VEKNVKESTDNVVEEV"
     gene            6472..7314
                     /locus_tag="PC700_001167"
     CDS             6472..7314
                     /locus_tag="PC700_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2580485.1"
                     /translation="MIINKILNNNVVITLDDNDEEVIVMGKGIGYQKSKGNLIDKTKV
                     NKVFRISNKEISNKLQELLNNIPIEHMKLSSEIIEYAQIKLNKKLNESIYISLSDHTY
                     SAIQRMKEGINVKNAILWEIKRFYKEEFEIGMKALDIIENKTGIKLPEDEAGFIAFHI
                     VNAQLSEGHTLASDITKLIQEVLSIVRYHFRIEFHEESVFYYRFIMHLKFFAQRLLLD
                     SAHEGETDKELLSIIKSKYNKEFECVVKIKNFIKKQYNYILTDDELIYLTIHLAKVVK
                     DSNM"
     gene            7459..9387
                     /locus_tag="PC700_001168"
     CDS             7459..9387
                     /locus_tag="PC700_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2580486.1"
                     /translation="MGKYKQLAQEIVNNVGGKENISGLVHCITRLRFTLKDESIANDN
                     VLKNMEGIVTVMKSGGQYQVVIGNHVEAVYKDVVEIIGLDDSSTSSETKKSGNILDKG
                     IDIISGIFQPVLGIMAACGMLKGFNALFVAMGLYSDVSGGYMVINAAGDALFTFLPLF
                     LGYTSAKKFGLKPMLGLALGAAVCYPAIQGSSISAGADVMYTLFKGTMFASPVYIDFF
                     GLPIVSIDYTGTVVPIIFIVYFASRCEKLFNKFVPDLVKFFFVPMLTLLITVPVALIV
                     IGPIATFGSTLISQIVISIRDFSPLLAGAIVGFTWQILVIFGMHWGFIPVYINNVMTL
                     GYDNVMMPFFACTFASSAVVLAIFFKTKDKKLKEMTIPNFISGIFGVTEPAIYGILLP
                     LKKPFIISCIASGIGGAFYGFFNLRKFITGGMGIFELPAMIEPDGGMGNLIVALSGIA
                     ISMVVAFVLTMILYKDEKVEESKKVRNKGKEEIKEVKSTKLEREIVASPIKGEVLKLS
                     DIEDAAFASGVLGQGVAIIPSDGKVVAPVDGVVTTLFPSLHAIGILSDTGVEVLIHIG
                     LNTIQLEGRGFEACIKQGDRITKGQTILNFDVDAIKELGYSTVTPIVITNSSQFLDVV
                     ETESKNIELEDNLITVLF"
     gene            9448..10875
                     /gene="ascB"
                     /locus_tag="PC700_001169"
     CDS             9448..10875
                     /gene="ascB"
                     /locus_tag="PC700_001169"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDF2580487.1"
                     /translation="MSFKDDFLWGGATAANQCEGGYNEDERGLANVDVCPTGKDRTAV
                     ITGKLKMFDFDDEHYYPAKTGIDMYHNYKEDIKLFAEMGFKVYRMSIAWSRIFPKGDE
                     ETPNEKGLQFYENIFKECKKYKIEPLVTITHFDCPMHLVKKYGAWRNRKMIGFYEKLC
                     NVIFRRYRGLVKYWLTFNEINMILHAPFMGAGICFEEGEDVEKIKYQAAHHELVASAI
                     ATKIAHEVDSNNLVGCMFAAGSVYPYSCNPNDVWEATKLDRENYFFVDVQSKGKYPNY
                     ALKYMEQKGITPEMEPGDIELLNKYTVDFISFSYYNSRCVRTDENADDMAEGNIFTSA
                     KNPYLSYSQWGWPIDPLGLRITLNHVYDRYEKPLFIVENGLGAKDIADENGYVEDDYR
                     INYLREHIKAMHDAVSIDGVDLLGYTTWAPIDLVSAGTGEMEKRYGFIYVDRDNSGNG
                     SLRRMKKKSFEWYKKVIASNGEDLY"
     gene            11286..12062
                     /locus_tag="PC700_001170"
     CDS             11286..12062
                     /locus_tag="PC700_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2580488.1"
                     /translation="MKYQHIIEVDKELCIGCGLCKNDCPVNNIIIENKKSVIKKQDCL
                     MCGHCAAICPTKAITLTGFDEPPIELTNKPKLDSDELLMAIKSRRSIRKFKDKEVSSE
                     IVKQIIEAGRYTPSAKNSQDVSYIVLDNKKSIYENEAVKFFRKIKPIANIAIKYSKEV
                     EIDDNFFFKHAPIAIMIITKDKISGSLAASNMALMAESYGLGVLFSGYFSDVANNSPK
                     LKKLLGLKRSNHVLTTLVIGYPDVKYRRTAQKEVATVRYL"
     gene            12078..12443
                     /locus_tag="PC700_001171"
     CDS             12078..12443
                     /locus_tag="PC700_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2580489.1"
                     /translation="MEKECQQRMQEITSKFKECRKAISAMGDETRQLILIALLESDFN
                     GIRVGEITKKTHLSRPAVSHHLKILKEAEIVNLRREGTKNYYYLDSKKSQWKSLTELI
                     NLIFVGIQHISENDIRKGD"
     gene            complement(12609..13358)
                     /locus_tag="PC700_001172"
     CDS             complement(12609..13358)
                     /locus_tag="PC700_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440038.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HDF2580490.1"
                     /translation="MILYFSGTGNSRYVARKIAQELNDELISLNQLIKEEKTDELISA
                     GKPFIFVCPTYAWRLPIVVTDFIKETKFLGDNRVYFVMTCGGDTAKSINYIQKLCKYK
                     EWQLKGMAEIKMPENYIALFSTTDKETSKQMIKEADKQIYRIISDIRNENEFETITPS
                     GLGGTIKSGIINTVFYKTIISAKGFHYTDKCIGCGKCVELCPLNNINLKNKKPVWKNS
                     CTHCMACICGCPTEAIEYKNKTQNRERYYLE"
     gene            complement(13803..14360)
                     /locus_tag="PC700_001173"
     CDS             complement(13803..14360)
                     /locus_tag="PC700_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDF2580491.1"
                     /translation="MRTLKYAILGLLNRNPMTGYDIVKEFNFQLAEFWNAKHSQIYPE
                     LKKLVNEKLIVYDIKISGDVLEKKLYTITEKGKDEFLKWLEKDEPMERTPKDIFRLRM
                     YFSNNLDIDTRLILLEHQLLQHQERITFLYSQMERYSEVPPLDSNDFGDYIVLDGAII
                     REQGAIKWLTNCINHHKNLDNTTLK"
     gene            14612..15910
                     /locus_tag="PC700_001174"
     CDS             14612..15910
                     /locus_tag="PC700_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HDF2580492.1"
                     /translation="MNLTQKRWIILIASCFINLCIGSIYAWSVFASPMAEHLNSLAGT
                     TFTAASLAIVFTVTNSVGPITMITGGMINDKFGPKKVVFVGGLIFGIGMILAGFSKGL
                     PMLILSYGIITGLGVGMVYGCTISNSIKFFPDKSGLVGGITTAAYGFSSVIMPPVANW
                     IINKYGVTSAFKSIGLVFLIIVCVSSFFIKKCPADFTPEGWVPPEKKGKVRVVINKNW
                     KEMLSTPIFYLMIIILTCGAFYGLMCVSLASSLATNMIGMTSSDSAIVVSILALFNTL
                     GRIVAGYISDKIGRINTLSGALVISIFGLLCLLFSREGDVATFYIGIIIIGICFGSFM
                     GIFPGFTVDKFGPKNNSVNYGIMFIGFAFAGYIGPAVMKNVYSVDGNYQRAFIIAIII
                     ALIGFILTFLYKYINKIQNLKIENLIKGDIKNGKKISKSM"
     gene            15882..17831
                     /locus_tag="PC700_001175"
     CDS             15882..17831
                     /locus_tag="PC700_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015394831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF2580493.1"
                     /translation="MERKYPNLCKPIKIGNVTFKNRMFSAPMGGTDITADCTIGSKST
                     AFYELRAKGGAGAVTISECMVHPETDGSHAYHLDLKIVDSLASFTYTADAIRRHGAIP
                     SVELSHSGQYSGTYLVDKDKKRGLSQWGVSPSVRPDGLEIKELTEEIIAEIVESYGNV
                     AALAKRAGFEMIMIHGGHGWLINQFLSPYFNKRTDKYGGSLENRVRFAKEVLDSVRKA
                     VGPGFPIEFRMSGSELFEGGYDLDYGTEIAKLLESRVDLLHVSAGTYQRGFAVTHPSM
                     FLEHGCNVYLAEEIKKHVNIPVATIGALNDPEMMEEIIASGKADVIYMARALLADHEL
                     PRKVMSNQDEKIVKCLRCFTCMAERATTSTRRCTVNPLIGRELDGTEVVSVLKPKKVL
                     IAGGGPGGLQAAITAVKRGHKVILCEKTNELGGILKGEQALPFKYKMYELGNTFGKIA
                     KDLGVEVRLNTTVTKEYVENENVDALIIAVGSEPLVPPIEGLDGENVVIVNDYYVEKE
                     KVTDEVIVLGGGLAGCEAAIHLAQEEKTVHLVEMRAELAPDANIRHRPILLEEIERQG
                     IYVYTEHKGLSVTTEGVVCMNKSGNKINIPGTSVICALGQKPRREVVDTLLDSAPYVA
                     QIGDCVRASTITNAVYQGHHAALDI"
     gene            18268..18405
                     /locus_tag="PC700_001176"
     CDS             18268..18405
                     /locus_tag="PC700_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2580494.1"
                     /translation="MINENLKSLRKIHQYTQEELAEKLNVSRQSIAKWESGVSQTKGY
                     Y"
     gene            18633..19007
                     /locus_tag="PC700_001177"
     CDS             18633..19007
                     /locus_tag="PC700_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HDF2580495.1"
                     /translation="MFNLIEMLFPIMFILIFIIIVFTFVKGIATWFKNNNSPRLTVSA
                     RIVAKRQNTTHNNQPNAGDISGAHGFHTISSTSYYVTFQVDSGDRIELSVSGSEYGVL
                     AEGDKGQLTFQGTRYLTFDREN"
     gene            19023..19670
                     /locus_tag="PC700_001178"
     CDS             19023..19670
                     /locus_tag="PC700_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580496.1"
                     /translation="MNKFLSQIRRIDNEISITRKIINTIFILCFGIVLGTFAKFLDTT
                     ASNTLPFIFEYLDISNFFGRFAIWLLIALYIAIYSHSSIRASLNVLVFFIGMVSSYYL
                     YSYFVAGFFPKNYAMIWLGFTVISPLLAFICWYAKGKSKISFILSVIIIAILFNFTFI
                     YGWIYFDVYSILEVIVFGCALIALKRNTFRETTYMILSAVVIAVILNMLVPFHFG"
     gene            20050..21129
                     /locus_tag="PC700_001179"
     CDS             20050..21129
                     /locus_tag="PC700_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDF2580497.1"
                     /translation="MNDMKFIETLKQKRNVCDYSQSRLAQELQINRQNLNEIENGKTK
                     ASKEMKHILLHYLDYCNCTQPFTLTIDYLRVRFPTTDALEIIKNVLAMKSEYFIHEDY
                     GMYAYEEQYIYGDISVNASKDSSMGVLLELRGMGCRDLEYVLQVRGIDWYCFLRCCID
                     YQGVFKRIDLAVNDMGGLLDIEILRERYYANKVWKRSRTHEAVDSGKLSGTHGDTAKT
                     FYIGSKNSSIYFCLYEKEKEQKSKGIKTDIKNRFEIRLKNGKAGQTVEQLVFSRNPEQ
                     TIASFILTQIDFPDYILWDIFLDNVTTSLPFIMTPVAVNMDKIKRWLERQVMPSLLMI
                     KEIEKKTGANYLEEIDRHTRLTEKQ"
     gene            21951..22164
                     /locus_tag="PC700_001180"
                     /pseudo
     CDS             21951..22164
                     /locus_tag="PC700_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
     gene            22276..23889
                     /locus_tag="PC700_001181"
     CDS             22276..23889
                     /locus_tag="PC700_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF2580498.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFERPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIVEKIFSLAYQGYGSAKITKVLREEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     ATNTANKTPYSYYACSYYGQFGKGTCSMHYIRYDVLYQAVLKRLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASAFKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITNLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEAITNEDNERIQEIEIYYRFIGKVD"
     gene            23918..24241
                     /locus_tag="PC700_001182"
                     /pseudo
     CDS             23918..24241
                     /locus_tag="PC700_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
     gene            24273..25460
                     /locus_tag="PC700_001183"
     CDS             24273..25460
                     /locus_tag="PC700_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HDF2580499.1"
                     /translation="MSKIVFFSILAYGHTNPTIEVVRELVDRGNEVLYYSFNEFKDKI
                     EGAGAKFICCDKYLPELLPGDEKKIGKDFPGLIEMIVDTTISLDEKVCRELKDFNPDC
                     IVSDSLSFWGKLFAQKLNIPYVCSTTTFAFNKHTAKLMKQNFIEIIRMIFGVRRINKK
                     IKLLQRKGYEVKNFISIVSNDNDTDTIVYTSKEFQPMVETFSSKYSFVGPSVSKLIIK
                     PKERKRKLIYISLGTINNKNVSFYKNCINAFKDSDVDVIMSVGRSTDIKSLGNIPNNF
                     EVKNSVEQIAILQKTDVFITHSGMNSVNESLYYGVPMVLFPQHSEQRMVAKRVVHLGA
                     GIMLKEDKSESIKKAVFQVINDNEYKENATKLSKSFYNAGGSKKAADVILQIICNSRQ
                     VDI"
     gene            25652..26056
                     /locus_tag="PC700_001184"
     CDS             25652..26056
                     /locus_tag="PC700_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HDF2580500.1"
                     /translation="MKKYKVIFIVLIIGIISVTGFVVYKNCIKPWKDAETYIDKYMVK
                     QDISKDNIKSITKEKAKKASYEGILYKVYYKDDPRYEYQYFYSDNYHALYVNKVMLQI
                     YDVENNNKLLRSNEELKSVKYPPIYLKYKPDK"
BASE COUNT         9872 a         3086 c         4823 g         8307 t
ORIGIN      
        1 tatctaatat tgatatttaa tatgaaagta ttgaaaatag acaatattaa ttatagtgag
       61 taaaaaatat aattggattt ttaaattgaa ttatttaaaa ggtatagtat gaaaaaaatc
      121 ttataactaa atatgagata gaaattataa aactttatac tgtgaaagag gtagatgcat
      181 gaaattaggg aaagattttt tatggggagg ggcaatttca gccaatcaaa gtgaaggatt
      241 ttatttagat ggaggaaaag gtttatctca gtttgataaa ctcccattaa atgataagag
      301 attaaaagat gtatgtttag atgaggataa tattttaaat gaaaataatc aatactatcc
      361 aagtcatata gggattaaat tttgtgaaaa ttacaaagaa gatatagcaa tgttagcaga
      421 aataggtttt aaatgtttta gattttctat atcttggtca agaatattcc caacaggaga
      481 agaaagtgaa ccaaatgaaa agggattaca actttatgat aatataataa aagaattaaa
      541 aaaatataat atagagccat taataacgat aagccacttt gatatacctt attctctagt
      601 tgaaaaatat tctgggtggg ctgataggag agtggttaat ttatatgtaa aatatgctga
      661 aattttgatg aaaagatata aagatgatgt caaatactgg attcctttta acgaaatgaa
      721 tatgataatt catattccat atataggtgc tgggttaact tttatagctg atgaaaatca
      781 acttcagaaa aaatatcaag cagcacacca tcaattatta gctaatgcaa aaactataga
      841 aataggaagg tatataaata aagaatttaa gtttgggtgt atgttagctg ctggaaaaac
      901 atatccatac acatgtaaac cagaagatgt atttgcaact ttagagcatg aaaggcataa
      961 tttgtttttt tctgatgttc aagttagggg tgtgtatcca agctttatga attattattt
     1021 aaaatcaaat caaataaaat taataataaa agaggaagat ttatcaacac ttaaggataa
     1081 tactgtagat tttgtatctt ttagctatta ttctagtgct tgtagttcag ctagagctga
     1141 aggtttagaa aaatcaaagg caaacggacc agaaaccata aaaaatccat atcttccaaa
     1201 atcaaatagt gtatggcaaa acgaccctgt agggttaaga ataactatga atcagttata
     1261 tgaaaggtat cagttacctt tatttatagt agaaaatgga ctgggtactc aagatattgt
     1321 aaatgaagat ggaaaaataa ttgatgatta tcgtatagat tacatgaaag aacatattaa
     1381 aaatatgatt cttggagtaa atgaagatgg aatagagctt ataggatatc ttatgtgggg
     1441 atgtatagat gtaaccagtg taagtgaagg aaagatgaca aaacgatatg gcataattca
     1501 tgttgattct gatgactatg gaagaggctc atttaaaaga actaaaaaac aaagtgcttt
     1561 ttggtataaa aaagtaatag aaacagatgg agaaatttta tttaatgata tggaaactca
     1621 tgcataattc attttaagaa aggagtgtta ttatgagttt aatatttact actaaggacc
     1681 aacttataga gcaagggttt agtaaggata caataaaatc caatgaaacg ttgtttacac
     1741 tagctaatgg acatttagga gttagaggga atattgaaga gtccgatttt aataatgagt
     1801 atgtagataa tatgggtaca tatgtaaatg gattttatga agaatctcca attacatatg
     1861 gagaaagcgc ttatggctat gcaaaacaaa atcagactat ctgtaagtta cctaatgctc
     1921 atatagtaaa tttctctatt gaaggagatt gctttgattt atcaaaaggt attacaagtg
     1981 aacatactag gatactagat ttaaatgaag gaattgttaa acgttcattt atatgggaaa
     2041 actctcgtgg tagaaaagtt aaagtatcta tagaaagatt agtatcacaa gatatagaag
     2101 aattaatgat gatttcatat acaataacac cattgaattt taatggaaaa ataaaattta
     2161 aaaataaaat ggaaaatagt atggagaagt ttaattgtga aaaagtagat gacccaagag
     2221 tggcatctat aaataagaag caatttaaaa ttaatgtaaa aaaatataac aataagtatt
     2281 ttatgaattt agttacagaa agaagtagat tagggttatg gtgtgggttt gattcatata
     2341 ttacttgtgg aaatattaca aatgagacct ataatgtttc agatgaatgt attgagcgag
     2401 aaatagaagt ctatgccaaa aaagatgaaa ggttaaattt agaaattgtt gtaggctata
     2461 gtagagtata ttctgattta aatctaaatg aagaacgttt aaaatcatta gaatgtctgg
     2521 agtcgttaaa atgtttagaa tcagaagcac atttaaaatc attagaattg gttttaagag
     2581 aagcatgtaa tattggattt gataaagtca aaaatattca gagaaagaaa atgaatgagt
     2641 tttggacaac ctctgatgta gagattgaag gcgataaaac tcttcaactg ggtataaaaa
     2701 ccaatttatt tcatatttat caatcagcag gaagagatgg aaagactaat atatcagcaa
     2761 aaggtcttac aggtgatggc tatgaggggc attatttctg ggatacagaa atgtatatat
     2821 taccattttt tatatatact caaccatcca ttgctagagc tttattagat tatagatact
     2881 caatcttaga tgcatcaaga gaaagagcta gggaacttgg aataaataaa ggttgcttat
     2941 attcatggag aactataaat ggtaatgaat gcagtgcata ttatccagca ggaacagcac
     3001 agtttcatat aaatgcagat atagcttatg gtattagaac gtattttgaa gcaactaatg
     3061 atgaagaatt tatgatgcaa aatggcttag aaattcttat agaaacagca agattttggg
     3121 ttgaatttgg agattatata gaattaaaaa ataataaatt ctgtataaat ggagtaacag
     3181 gaccagatga atatacagca atagtaaaca ataattacta cactaattta atggctaaat
     3241 ataatttaat atatgcagta cagatggtag ataaatttaa gcataattta ttttcaaatg
     3301 aagtatttag aaaagttaaa ttacaagaat atgaagtaaa agactggata aatgccgctc
     3361 aaaatattta tttaccatat gatgaagaga agaaaataag caaacaagac gattcatttt
     3421 tagaaaaatc agtatgggat tttaaaaata caccaaaaga aaaatatcca ttacttctca
     3481 attatcatcc attaacaata tatagatatc aagtaaataa acaagcagat actgtacttt
     3541 cagcgttgct tttccctgat gagtttagct tagaacaaaa gaagagagat tttgaatact
     3601 atgaaaaaat aactactcat gattcatctt tgtcaaggtc aatatttgga atgatggcaa
     3661 gtgaaattgg aaatcatgaa aaagcttata actattttat ggacacagca ttaatggata
     3721 ttaataacct acaaaaaaat acaagtgatg gaatacatgc tgctaatatg ggtggaacat
     3781 ggatgagttt agtatatgga tttgcaggaa tgaaaataaa aaaggataaa ttaagttttg
     3841 aaccaaggat acctaaacac tggaaaaata ttaaatttaa tatattattt agagataatt
     3901 tagtttctgt aaatatagaa gaatataaat cttattataa gttaatcaaa ggtaaaaaca
     3961 taagcatatt acatgataat aaagaaataa aattatcaaa gaacataata gaagttaata
     4021 attgttttaa agggagtaat taatatgaaa gcagtcttat ttgatcttga tggagttata
     4081 acagatactg ctaagtatca ttttgaagct tggcatgaga tagcaaaatc gctagatata
     4141 gaaatagatg aaacttttaa tgaaaaatta aaaggcataa gcagggaaga atcattagat
     4201 aaaatattgt tatctgctaa taaagtagat agttatacta aagaggaaaa gttaaaggtg
     4261 tgtgaagaaa aaaataacat ttatataaag ttaataaact cattaacata taaagatata
     4321 ttaccaggga taaatgagct tataaaagaa cttaaaaaaa ataatgtgaa attgggaata
     4381 gcatctgcta gtaagaatgc acctaggata ttagaatctt tgggaataaa agatgaattt
     4441 gattgtatag taaatccatc cttattagaa aggggaaaac catatcctga tatttttatt
     4501 gaagggagca agatgctaaa tttaaaacaa tatgattgtg taggaataga agattcttat
     4561 tcaggtatta aagctataaa tgctgccaat atgacatcaa ttggtgtggg gaaaaaagaa
     4621 gtactaggaa aatgtgacca tattgtagaa gatactagct tattaaatta caatacacta
     4681 ttacaagctt ggagtaaaaa gattaatttg gattaaaatt atattaaaaa ttattttaaa
     4741 agagaggata aggtatgaag aataaatatg aagaagtagc tcaattgata attgatggac
     4801 ttggaggaaa ggataatata attgacttaa ctcattgtat gacacgatta aggttcatct
     4861 taaaagatga aaagaaagtc aatgcagata aattaaaatc tatagataag gtagttggag
     4921 taaactcaac ttcaactcaa tatcaggtca taataggtaa tgaggtaggt gttgtattta
     4981 atgcaataat aaataagggg ataaatacca caggagatat taaaagcgat ataaaaaatg
     5041 aaaataaaaa aaatagtatt ttaaataata taatagatac aataactggt tgtatgacac
     5101 ctatgatacc agcccttaca gcatctggga tgataaaagt tatattatct ctttgtacca
     5161 cttttaattg gctatcatca aattcaagta catatagagt attagatata ataggagatg
     5221 tagcatttta ctttatgcct ataatacttg caataaatgc agctaaaaaa tttaatgtta
     5281 atacatcaat agcagttata gtagtaggtg tgtttttaca tccaaatttc tctgcatggg
     5341 tatcaagtgg ggaccctata tcatttatag gagtaccaat tcaaggtgtt atatatgcag
     5401 catctgttat ccctgcactt ttaacagtat ggatgatgtc atatatagaa aaattcatta
     5461 ataaattaac acctagcatg ttaaaaacaa ttttaaatcc aactttagta ttattaataa
     5521 gtgcgccaat agcattaata gtaattggac caataggaaa tttcttagga gagggattag
     5581 ctagtataat aaatttacta caaggaaggt taggatttat aatggtttgt ttattagcag
     5641 ctgcaatgcc atttatcgtt atgacaggga tgcatcatgc attgactcct atattcatat
     5701 cagcatttgc aactacagga caagaatcgc taatattaat atctcaagtt tgtgctaatt
     5761 tagcgcaagg tggcgcgaca ttagctgtag cattaagaag caaagatagt aagatgaaac
     5821 agttagcatc agctgcatgg atatcagcta tcatgggtat aactgaacca gctctttatg
     5881 gagttacatt gaaacttaaa aaacctgcta tgttagcatc tataggagca ggaatcgcag
     5941 ggttatttgc aggaattact catgtgactt tatatgttcc acaaaatagt ataatggcag
     6001 tacttggatt atcaggggaa aagggaattt caaatatagt aaatggactt atagttatat
     6061 ttatagcatt ggttgctcca tttattatga catatgtttt tggagttgaa aaaaatgtta
     6121 aggaaagtac agataatgta gtagaggaag tataatatta actatttaca ttaatagaat
     6181 ttttattatt attgactata acttgttttg tatttatatt gatgcaaaga aataagttaa
     6241 tattaatgat aataattatt ataactgaca aaaagcaaca caaaagattg atgaagtata
     6301 tggaaataaa aagttgacaa aatatgtctg atgttttatc atattaattg gaattaggta
     6361 gtaattgttt aagtacaagc taagcttatt cattaataat agcagtatcc taggaaggag
     6421 tatgcccatt attatggata agcttttttt atgcttaaaa ggaggataat catgataatt
     6481 aataagatat taaacaataa tgttgtaatc acattagatg ataatgatga agaagttatc
     6541 gttatgggta aaggaattgg ttatcaaaaa tcaaaaggca atctaataga taaaactaaa
     6601 gtaaataaag tatttagaat atcaaataaa gaaatatcaa acaaactaca agaattactc
     6661 aataatattc ctatagaaca catgaaatta tcaagtgaaa ttatagaata cgcacaaata
     6721 aagttaaaca agaagctaaa tgaaagtatt tacatatcct taagtgacca tacatattct
     6781 gcaattcaaa gaatgaaaga aggaataaat gtaaaaaatg caattctttg ggaaattaaa
     6841 agattttata aagaagagtt tgaaattgga atgaaagcac tagatattat tgaaaacaaa
     6901 acaggtataa aattaccaga agatgaggct ggatttatag cctttcatat agtgaatgct
     6961 cagttaagtg aaggtcatac tttggcatct gatattacga agttaataca agaagtactt
     7021 agcatagtta ggtatcattt taggatagaa tttcatgaag aatcagtgtt ttattataga
     7081 tttataatgc atttaaagtt ttttgcgcaa agattgcttt tagatagtgc acatgaaggt
     7141 gagacagaca aagaattact aagcattatt aaatctaaat ataataaaga gtttgaatgt
     7201 gtagttaaaa ttaaaaattt cattaaaaaa caatataact acatattaac agatgatgag
     7261 cttatatatt taacaataca tttagctaaa gtagttaaag actcaaatat gtaagtgaaa
     7321 taatttttca gtagtatatt aagaatgatt gaaaggagtg atactaacaa aattatacct
     7381 atggttggat ggtaacattg agttggccaa accttcaaat ttcaaaaaat taatttaaac
     7441 gaaatatgga ggagaaaaat gggaaaatac aaacaattag cacaagagat agtaaacaat
     7501 gttggtggaa aagaaaatat tagtggatta gtacactgta ttacaagatt acgttttact
     7561 ttaaaagatg aaagtatagc aaatgacaat gtattaaaaa acatggaagg tattgttact
     7621 gtaatgaaaa gtggaggaca atatcaagta gttataggaa atcatgttga agctgtctat
     7681 aaagatgtag ttgaaataat tggtttagat gattcaagta caagttcaga aactaaaaaa
     7741 tcaggtaata tactagataa aggtatagat ataatatcag gaatttttca gccagtgtta
     7801 ggaataatgg cagcttgtgg tatgctgaaa ggatttaatg cattatttgt agcaatggga
     7861 ttatatagtg atgtatctgg tggttatatg gtaataaatg cagcaggaga tgcattattt
     7921 acattccttc ctttgttctt aggatatact tcagctaaga agtttggatt aaaaccaatg
     7981 ttgggattag cactaggagc tgcagtatgt tatcctgcta tacaaggtag cagtatatca
     8041 gcaggagcag atgtgatgta cactttattt aaaggaacaa tgtttgcatc accagtatat
     8101 attgatttct ttggactacc aattgtatca attgattata ctggaacagt cgtaccaatt
     8161 atatttatag tgtattttgc ttctaggtgt gaaaaattat ttaataagtt tgttccagat
     8221 ttagttaagt ttttctttgt gccaatgctt acattattaa taacagtgcc tgttgcactt
     8281 atagttatag gtcctatcgc aacatttggt tctacactta tttcacaaat tgtaatttca
     8341 ataagagatt ttagtccact attggcaggt gctatagtag ggtttacttg gcaaattctt
     8401 gttatttttg gtatgcactg gggatttatt cctgtatata taaacaatgt tatgacttta
     8461 ggatatgata atgttatgat gccattcttt gcttgtacat ttgcaagttc ggcagttgtg
     8521 ttggcaatat ttttcaagac taaagataaa aaactaaaag agatgacaat accaaacttt
     8581 atatctggta tatttggagt tacagagcca gcaatttatg gaatattatt gcctttaaag
     8641 aaaccattta taataagttg tattgctagt gggattggag gcgctttcta cggattcttt
     8701 aatcttagaa aatttataac tggaggtatg ggaatatttg aacttccagc aatgattgaa
     8761 ccagatgggg gaatgggcaa cttaatagta gctctttctg gtatagctat atcaatggtc
     8821 gtagcttttg tattaactat gattttatat aaagatgaga aagtagaaga gtctaaaaaa
     8881 gtaagaaata aggggaaaga agaaataaaa gaagtcaagt ctactaaatt agaaagagaa
     8941 atagtagcaa gtcctataaa aggtgaagta ttaaaattaa gtgatataga ggatgcagca
     9001 tttgcatctg gtgtactagg tcaaggggtg gcaataatac ctagtgatgg aaaagtagtt
     9061 gctccagtag atggtgtagt tacaacctta tttcctagtc tacatgctat agggattctt
     9121 tctgatacag gtgttgaagt gcttattcat attggattaa atactattca attagaaggg
     9181 agaggttttg aggcatgtat aaaacaaggt gatagaatta caaaaggtca aactatatta
     9241 aactttgatg ttgatgcaat aaaagagttg gggtatagta ctgtaactcc aattgttatt
     9301 acaaattcat cacaattttt agatgtagta gaaacagaaa gtaaaaatat tgaattagaa
     9361 gataatctaa ttacagtctt attttagtaa aaattgaggc attagcataa gctagtgctt
     9421 tggttataat ttaaggaggc tttgactatg agttttaagg atgatttttt atggggagga
     9481 gctactgcag ccaatcaatg tgaaggtgga tacaatgaag acgaaagagg attggcaaat
     9541 gtagatgtat gtccaacagg aaaagataga acagcagtta ttacagggaa actaaaaatg
     9601 tttgattttg atgatgagca ttattatcca gctaaaactg gtattgatat gtatcataat
     9661 tataaagaag atataaagtt atttgctgaa atgggattta aagtatatag aatgagcatt
     9721 gcatggagtc gaatatttcc aaaaggtgat gaagaaacac caaatgaaaa gggacttcaa
     9781 ttttatgaaa atatatttaa ggagtgtaaa aaatataaaa tagagccttt agtcactatt
     9841 acacactttg attgtccaat gcatttagtt aaaaagtatg gtgcatggcg taatcgaaaa
     9901 atgataggtt tctatgagaa attatgcaat gttatcttta gaagatatag aggattagtg
     9961 aagtactggc taacctttaa tgaaataaat atgattttac atgctccatt tatgggtgct
    10021 ggtatttgct ttgaagaagg tgaagatgtt gaaaagataa aatatcaagc tgctcatcat
    10081 gagcttgtag ctagtgcaat tgcaactaaa atagcacatg aagttgattc aaataactta
    10141 gttggatgta tgtttgcagc tggaagtgta tatccttata gttgtaatcc taatgatgta
    10201 tgggaagcta caaagttaga tagagaaaat tacttctttg tggatgtaca atctaaaggt
    10261 aaatatccta actatgcgtt aaagtatatg gagcaaaaag gtattacacc agaaatggaa
    10321 cctggtgata ttgaattatt gaataaatat acagtagatt ttatttcatt ttcatattac
    10381 aatagccgtt gtgttagaac tgatgaaaat gcagatgata tggctgaagg aaacatattt
    10441 acatctgcta aaaatccata tctttcatac agtcaatggg gatggccaat tgacccatta
    10501 ggtttgagaa ttacactaaa tcatgtatat gatagatatg aaaagccact atttattgta
    10561 gaaaatggat taggtgctaa agatatagct gatgaaaatg gttatgtaga agatgattac
    10621 agaattaatt atctaagaga acatataaaa gctatgcatg atgcagtttc tatagatggg
    10681 gtcgatttat taggttatac tacatgggca cctattgatt tagtaagtgc tggtacaggt
    10741 gaaatggaaa agcgttatgg atttatatat gtagatagag ataatagtgg taatggaagt
    10801 ctaagaagaa tgaaaaagaa aagttttgaa tggtataaga aagtaattgc ttcaaatggt
    10861 gaagatttat attaaacaag aaatctatgt taaataagaa atttgtatta aataagaaga
    10921 gtataaaaat taataataag tataattttt aactttttta actatagaaa atcaatacag
    10981 aattatttta cattgtattt atgcaatagt atgattcaca aataaaattt tagaaaataa
    11041 aattgttaag aagtatggga agtaagtgca atttactttg taataaaaga tgaaaaaggc
    11101 gagcttaggt tttaagcttg tcttttttat tatgtataaa ctatgatact ttaaaattct
    11161 atgaaaaatt tatttaatag agaaattaaa aatttaatgg taatcaaatt ctttaccaat
    11221 gaatataaaa aatgtctacg tgttataata gtttaaacta ataaacgaaa ggtagatgaa
    11281 tgaatatgaa ataccaacat ataatagaag ttgacaaaga actttgcatt ggatgtgggc
    11341 tttgtaaaaa tgattgtcca gtaaacaata taattataga aaataaaaag tcagtaatta
    11401 aaaaacaaga ttgtctaatg tgtggacatt gtgcagcaat atgtcccaca aaagctatta
    11461 ccttgacagg atttgatgaa cctcctatag aacttacaaa taagccaaag ctagattcag
    11521 atgaattact aatggcaatt aaatctagaa gaagtatacg taaatttaaa gacaaagaag
    11581 tttcttcaga aattgtaaag caaattattg aggcaggtag gtatacccct agtgctaaaa
    11641 attcacaaga tgtatcatat attgttttag acaataaaaa gtcaatttat gaaaacgaag
    11701 cagtgaaatt ttttagaaaa ataaaaccta ttgcaaatat agctattaaa tattcaaaag
    11761 aagttgagat tgacgataac tttttcttta agcatgctcc tattgcaata atgataatta
    11821 caaaagataa gattagtggt tcacttgcag catcaaatat ggcattaatg gcagagtctt
    11881 atggtctagg tgttttattt agtggatatt tttcagatgt agcaaataat tcgccaaaat
    11941 taaaaaagct tttaggtcta aaacgcagta atcatgttct tacaacatta gtaattggat
    12001 acccagatgt taaatataga cgtactgcac agaaagaagt agcaactgtt agatacttat
    12061 aagagagggt ggattttatg gaaaaagaat gtcaacaaag aatgcaagaa ataacatcta
    12121 aatttaaaga atgtcgcaaa gctatctctg ctatgggtga tgagactaga caattgattt
    12181 taattgctct cttagagagt gattttaatg gtatacgagt gggagaaatc acaaaaaaaa
    12241 cacatctttc tcgtcctgct gtatctcatc atcttaagat attaaaagaa gcagagattg
    12301 ttaatttgcg tagagaagga acgaaaaatt attattatct tgattcaaaa aaatcacaat
    12361 ggaagtcact aacagagcta ataaatctta tatttgttgg aatacaacat attagtgaaa
    12421 atgatataag aaaaggtgat taaattatac ttgatttaat acaaaaagat tgatttagga
    12481 actaaaaagt tcactttgag acactatctt ttgaactata cccatcttag gagacagtta
    12541 aaatatgtaa aactgtataa aacaatgggt atagttcatt ttataatggt ttaaatatta
    12601 agaatatatt attctaaata gtatctttct ctgttttgag ttttattttt atactcaata
    12661 gcttcagtgg ggcaaccaca aatacatgcc atacaatgag tacagctatt tttccaaact
    12721 ggctttttgt tttttaaatt aatattattt aaaggacata gctctacaca ttttccacag
    12781 ccaatgcatt tatcagtata atgaaatcct ttggcactaa taattgtctt ataaaaaact
    12841 gtatttatta taccactttt aattgtacct cctaagccac taggagtaat agtttcaaat
    12901 tcattttcat tgcgtatatc agaaataatc ctatatattt gtttatctgc ttctttaatc
    12961 atttgttttg atgtttcttt gtctgttgta gaaaataaag ctatataatt ctctggcatt
    13021 ttaatttctg ccataccttt taattgccat tccttgtatt tacatagttt ttgaatatag
    13081 tttatggatt ttgctgtatc tcccccacaa gtcattacaa agtaaactct gttgtctcct
    13141 agaaattttg tttcttttat aaaatctgta accactattg gcaatctcca tgcataagta
    13201 ggacatacga atatgaatgg tttacctgct gatattaatt catctgtttt ttcctctttt
    13261 ataagttgat ttagtgatat cagttcatca tttaattctt gtgcaatttt acgagccaca
    13321 tatcggctat taccagtacc gctaaaatat aaaatcataa taatatctcc ctaactattt
    13381 tttattttaa ctcttaattt tttatttatt atagtacttt attgaaatat tctattcaaa
    13441 tgtatgtaaa atttagtagt taagaaaatt ataacacatt agtaattttt atattcattg
    13501 gtaaagaatt tgattaccat taaatttagt aacaaaatat caactggatt tttatataag
    13561 aagagatagt aattttcatg tgaaagattg taaatataaa gatgattttg aagggcaata
    13621 agaagataaa attgatagct taataaaaaa tatttaactt ttatattaac tgttttaaat
    13681 attataatta tctatcttga ataatcaacc atagaagact gtatacaaag acaaagttgt
    13741 gtacagtctt ttttataaat ttaatgtcta taattaaata cactattaaa atcaataagt
    13801 tattatttca aagtagtatt atccaaattt ttatgatggt taatacaatt tgttagccat
    13861 tttatagcac cttgttccct gataatagct ccatcaagaa ctatataatc accaaaatca
    13921 ttactatcaa gaggaggaac ttcactataa cgctccattt gactgtacaa gaacgtaatt
    13981 ctttcttggt gttgaagcaa ttgatgctct agtaaaataa gcctagtatc tatatctaaa
    14041 ttattagaaa aatacattct aagacgaaaa atatccttag gggttctttc cattggctca
    14101 tccttttcaa gccatttcaa gaactcatct ttaccttttt ctgtgattgt gtataacttt
    14161 ttctctaata catcaccact aattttaata tcatatacaa taagcttttc atttaccaat
    14221 ttttttagtt caggatatat ttgactatgc tttgcattcc aaaactcagc taattgaaaa
    14281 ttgaactcct ttacgatatc ataaccagtc ataggatttc tatttaataa tcctaaaatt
    14341 gcatacttta aagttcgcat aaatacacct ctttctttaa ttacatactt attataacac
    14401 gatatattta gtttgaatta attaatatag ctatgtttta ataagaactt ttaaattatg
    14461 aggaacaaaa aataaataaa actatatata aaaatgaaat tatgtaaata taaacatgta
    14521 agtatttaca tgtttatatt tacatataga aaaaattgtg ataatcttaa ctaagaaatt
    14581 ctatttaata aatttatcga ggaggagagt aatgaattta acacaaaaaa gatggattat
    14641 attaattgca agttgtttca tcaatttatg tattggttca atatatgcgt ggagtgtatt
    14701 tgcatcacct atggcagaac atttaaatag tcttgctgga acaactttta cagctgctag
    14761 tttagcaatt gtatttacag ttacaaactc agttggacct attactatga ttacaggggg
    14821 aatgataaat gataaatttg gtcctaaaaa agttgtattt gtaggaggat taatttttgg
    14881 tataggtatg atattggcag gtttctcaaa aggattgcct atgttgattt tatcatatgg
    14941 gataataaca ggtcttgggg ttggcatggt ttatggatgt acaataagta attcaattaa
    15001 attttttcca gataaaagtg gattagttgg tggtataaca acagcagctt atgggtttag
    15061 ttcagtaata atgcctccag ttgctaattg gataattaat aaatatggag taacttctgc
    15121 atttaaatca ataggtttag tatttcttat aattgtatgt gtttcttctt tctttatcaa
    15181 aaaatgtcca gcagatttca caccagaagg atgggtacca ccagagaaaa aaggaaaagt
    15241 aagagttgtt attaataaaa attggaaaga aatgctttca acacctatat tttatctaat
    15301 gattataata ttaacttgtg gagctttcta tggattaatg tgtgtgtcat tagcatcttc
    15361 tctagcaaca aatatgattg ggatgacaag ttcagattca gcaatagtgg tttcaatttt
    15421 agccttgttt aatacattag gccgtattgt tgcaggatat atatcagata aaattggtag
    15481 aataaatact ttatcaggtg cattagttat ttcaattttt ggtttattat gtttgttatt
    15541 ctcaagagaa ggagatgtag caacattcta tattggaata ataattatag gtatatgttt
    15601 tggttcattt atgggaattt tcccaggatt tacagtagat aaatttggac ctaaaaataa
    15661 tagtgtaaat tatggaatca tgtttattgg ttttgctttt gcaggatata ttggacctgc
    15721 agtgatgaaa aatgtatatt ctgtagatgg caattatcaa agggctttca ttatagctat
    15781 aattatagct ttaataggtt ttatattaac atttttatat aaatatataa ataaaataca
    15841 aaatttaaag atagaaaatt taataaaggg agatataaaa aatggaaaga aaatatccaa
    15901 atctatgtaa gccaattaaa attggaaatg taacattcaa aaacagaatg ttttcagcac
    15961 ctatgggggg aacagacata acagcagatt gtacaattgg ctcaaaatca acagcctttt
    16021 acgaattaag agcaaaaggt ggagcaggtg cagttacaat tagtgaatgt atggttcatc
    16081 cagaaacaga tggttcacat gcatatcatc tagacttaaa aatagttgat tccttagcaa
    16141 gttttactta tacagcagat gcaattcgcc gtcatggagc aataccaagt gtagaactat
    16201 ctcattcagg acaatattca ggaacttatt tagttgacaa agataagaag cgaggattat
    16261 cacaatgggg agtaagtcct agtgttcgtc ctgatggatt agagataaaa gaacttacag
    16321 aagaaataat tgctgaaata gtagagtcat atggaaatgt tgcagcattg gctaagagag
    16381 cagggtttga gatgattatg attcatggtg gacatggctg gctaatcaat caattcttat
    16441 caccttactt taataaaaga actgacaaat atggtggttc tttagaaaac agagttcgtt
    16501 ttgctaaaga agttttagat agtgttcgta aagcagtagg tccaggattt ccaatagagt
    16561 ttaggatgag tggttctgaa ttatttgagg gagggtatga tttagattat ggaacagaaa
    16621 ttgctaaatt attggaatca agagtagatt tacttcatgt atcagcaggt acataccaaa
    16681 gagggtttgc agtaacacat ccttctatgt tcttagaaca tggatgtaac gtatatcttg
    16741 cagaagaaat taaaaaacat gtaaatattc cagttgctac tataggagca ttaaatgacc
    16801 cagaaatgat ggaagagata attgctagtg gaaaagcaga tgttatttac atggctcgtg
    16861 cattactagc tgaccacgaa ttaccaagaa aagttatgag taatcaagat gaaaaaatag
    16921 tgaaatgcct aagatgcttc acttgtatgg cagaacgtgc taccacttct acaagacgct
    16981 gcactgttaa tccacttatt ggacgtgaat tagatggaac tgaagttgtt tcagtattaa
    17041 aacctaaaaa agtattaata gcaggtggtg gaccaggagg attgcaagca gccattacag
    17101 ctgtaaagcg tggacataaa gtaatacttt gtgaaaaaac taatgaatta ggtggaattt
    17161 taaagggaga acaagcactt ccatttaagt ataaaatgta tgaattagga aatacttttg
    17221 gaaaaatagc aaaagattta ggtgttgaag tacgtttaaa tacaacagtt acaaaagaat
    17281 atgttgaaaa tgaaaatgtc gatgcattaa ttatagcagt agggtctgaa cctcttgttc
    17341 caccaataga gggtcttgat ggagaaaatg tagtaatagt taatgattat tacgtagaaa
    17401 aagaaaaagt cactgacgaa gtaatagttt taggtggagg attagcagga tgtgaggctg
    17461 caattcactt agctcaagaa gagaagactg ttcacttagt agagatgaga gcagaacttg
    17521 ccccagatgc aaatataagg catcgtccaa tattattaga agaaatagaa agacaaggta
    17581 tatatgtata tacagaacat aagggtttaa gtgtaacaac agaaggtgta gtatgtatga
    17641 ataaatctgg gaataaaata aatattccag gaacctcagt aatatgtgca cttggacaaa
    17701 aaccaagaag agaagtggta gacactttac ttgattctgc tccatatgtt gctcaaattg
    17761 gagactgtgt tcgtgcatct acaataacaa atgcagtata ccaagggcat catgctgcgc
    17821 tagatatata gttaaaaaga cgtagatagt attgttttat tataaaaaag gagtgtctca
    17881 aaatgaactt tttagttcat gagacacttt tttacatgaa attaaatata tttttatcat
    17941 ttcaacaatg aaaaaagatt atctttattt tgagacagca cttttgttat tatgatttta
    18001 attcttattg caagttttca tttgattgat gagtaaaact ttttaagatg tagaagtatt
    18061 taacaatgta aatgacccta acttaaatac tttagcgaag gttgacagat ttgtgtttgc
    18121 ttttatctat tataacttaa ggaaacatgt tacctgaaca ataaatttat ataataaaag
    18181 tatataaata tatatatgaa tgttaagatt agttgataga atttaaataa taaatccaat
    18241 attataaaca taggaggtat gaaaaatatg attaatgaga atttaaaaag tctaagaaaa
    18301 atccaccagt atacacaaga agaattagct gaaaaactta atgtttctag acaatcaata
    18361 gcaaaatggg aaagtggagt ttcccaaaca aaaggatatt actgaaataa cctgtgttta
    18421 cctgcttgta gagaaaagaa tattgctatt aagccagaga tactacataa atgtgttggt
    18481 atctcttttt cgttaatgca acacagtaaa ttatttcatt ttcctaatta ttttagactg
    18541 ataagtaact ttttaaatga agaaaagaaa tgacaatact taaaggagca tggtatactt
    18601 aaaggagaat ttcattaagg aggaaaaaga gattgtttaa tctaatagaa atgttatttc
    18661 ctataatgtt tatactgatt tttataataa ttgttttcac ttttgtgaaa ggaatagcta
    18721 cttggtttaa aaacaataat tctcctcgct taactgtgtc agcaagaata gtagcaaaac
    18781 gtcagaatac cactcataac aatcaaccaa acgctggtga tatatcagga gcacatggat
    18841 ttcatacaat ttccagcacc tcgtattatg tgacatttca agtagatagt ggtgatagaa
    18901 tagagctgtc tgtatctggc tcagagtatg gagtgcttgc agagggagat aaaggacagc
    18961 tgacattcca aggcacaaga tatttaacat ttgataggga aaattgaatt tagaggtgca
    19021 ttatgaataa gtttttgagt cagataaggc gtatagataa tgaaatatct attactagga
    19081 aaattataaa tacaatattt atactgtgtt ttggaatagt tttaggaact tttgcaaaat
    19141 ttttagatac tacagcaagc aatactttac catttatatt tgaatattta gatattagta
    19201 atttttttgg tcgttttgct atatggttat tgattgcttt gtacattgca atttatagtc
    19261 attcttccat aagagcatca ttaaatgtgt tagtattttt cattggaatg gtttcaagtt
    19321 attacctata ttcgtatttt gttgctggat tttttccaaa aaattatgca atgatttggc
    19381 ttggttttac tgtcatttct ccattgttag cttttatttg ttggtatgct aaggggaaaa
    19441 gcaaaatatc ttttatactt tcagtaataa taattgcaat tttatttaat tttacattta
    19501 tttatggttg gatttatttt gatgtgtatt caattttaga agtgatagtc tttggttgtg
    19561 cattaatagc tttaaaacgt aacaccttta gggagacaac gtatatgata ttgagtgcag
    19621 ttgttattgc tgttatactt aatatgttag ttccatttca ttttggttag gcagatttta
    19681 agattattat agatagaata taaatttcac gaaccgccga agcgatccgc cagcggtggc
    19741 ggtcgtaaga catatcgcca cgccagcttg ctggcgaggt ggcggttcac tttggcggtt
    19801 tggcgagcct gcgagctttc taagcccccg ttatctaaca gggggggact attacaaaag
    19861 acaagagcaa aaacaaatat aaaactgctg atatgcaacg atttataagg ttgtaagaac
    19921 ttatttcact ttacacccta ttctttctac ccgacaggtc taaaacagca tatcaataga
    19981 aaataaatat aagatacaag gtgtatgctc ctatgagtat atgccttttt ttgattggag
    20041 gaatgaagca tgaatgatat gaaatttatt gaaacattaa agcaaaagag aaatgtctgt
    20101 gattattcgc aatcacgact ggcacaggaa ctacaaatca acaggcagaa cttaaatgaa
    20161 attgaaaacg gaaaaacaaa agccagtaaa gaaatgaaac atatactctt acattatctt
    20221 gattattgta attgtacaca gcctttcact ttaacgattg actatctgcg tgttcgtttt
    20281 cctaccacag acgcattgga aattataaaa aatgtgctgg caatgaaaag cgaatatttt
    20341 atacatgaag attacggaat gtatgcttat gaagaacagt atatctatgg ggatattagt
    20401 gtaaatgctt ctaaagatag ttctatgggt gttttattgg aattaagagg tatggggtgt
    20461 cgggacttag aatatgtttt acaggtaaga gggatagact ggtattgctt tttaagatgt
    20521 tgtatagatt atcaaggtgt ttttaaacgc atagatttag cagtcaatga tatgggcgga
    20581 ttgctggata tagaaattct aagggaacgt tattatgcta acaaggtatg gaaacgttca
    20641 agaacccatg aagcagtaga cagcggaaaa ttatcgggta cacatggaga tactgcaaaa
    20701 actttttata tcggttcaaa gaatagttct atctactttt gcttgtatga aaaagaaaag
    20761 gaacaaaaaa gcaagggtat aaaaacagat attaagaacc gcttcgaaat tcgtttaaaa
    20821 aatggaaaag caggacaaac ggtagagcaa cttgtttttt caagaaatcc cgaacagacc
    20881 atagcaagct ttattctcac acagatagat tttcccgatt atattttatg ggatatattt
    20941 ttagataatg taacgacctc acttccgttt attatgacac ctgttgctgt taatatggat
    21001 aaaataaaac gctggctaga aagacaggtc atgccctctt tattgatgat aaaagagatt
    21061 gaaaagaaaa caggagcaaa ttatctggaa gaaatcgaca ggcatacaag gctaacagaa
    21121 aagcagtaat taaagattaa acaaatgaca acagacatag cagatatgat tgagaaagat
    21181 accgctgttc cacaagggaa tgacggtatt ttttaatttc ttcaaaaaaa ctgcaacaaa
    21241 atgactgctt gtgtacagat atggtgcgag gaaaggaaat ctaacttttt ttgaaaacag
    21301 gtcattaaat cggtggctgc tgtccctata tgatgtgaaa gaagaaacga tttttaattt
    21361 caacttaaca tttgacagtt ttcattccct atatggagca aagaaaatct tttattccat
    21421 agttggcagt tacggtgttg catgggtagt tagggtgtga aaagaaaaac agattttttt
    21481 catcaccaac ttagcaaact gatacttgct ttccctatat ggtgcaggaa aagagagttt
    21541 aactattttc atttcaactt agcaactgga cgtttgctgt acagatatgt agtgaaaaga
    21601 gaaaaagttt tgaatattgg ttacgcttcg gctcttttcc aacgcggtat ataggaaaga
    21661 cgattttctt ctttcatgtt ctttgacaac tgaataaagc aattcaatac gtttgacaga
    21721 cagcgatcca ttgaagcaga tacgccatga ccttttccct gcaaaagcga gcgaacccta
    21781 tttgtgttcc tgtaaaagac ttgtcctctt ttatcgccat gacctgttct gtctgataaa
    21841 tgatactccc gtacagccac agcttgaacg ttcagagcgt cgcacgcaat gggtacggct
    21901 acataagaac tatgcagagg tggaattcct gtgggctatg acaaatgccg ttgaattgct
    21961 tagaaaaaag attatggaaa gggggtatgt gttattgctg atattgtaaa aacaatgcaa
    22021 gaaaagacaa ataataagcg tagcattggc aacagaggaa aaacaaagat tgttgtaaaa
    22081 gagcattttt ccgaaaaggg aaagacaatg gaggaacttc tgactgatgt tatgctggaa
    22141 aaagcaaagc agacaatcgc ataaaattgg tgcagttgta agacatagat tgaatggcaa
    22201 aaagtacccg tgttatactt tacttgcaag caggtattgt aaacacgggt tagttataaa
    22261 ggaggataac tgacaatgaa acaacagatt tacaatactg cactttatct taggttaagc
    22321 cgagatgatg aattacaggg cgaaagttcc agcattacca cacaaagaag tatgttgcgt
    22381 ctatatgcaa aagaacatca tttgaatgtg attgatgaat atattgatga cggctggtcg
    22441 ggaacaaatt ttgaaagacc gagttttcaa agaatgattg aggatataga ggcaggaaaa
    22501 atcaactgtg ttgtaacgaa agacctttcc cgacttggca ggaactatat tatgacagga
    22561 caatatacag aattgtattt tcccagccat aatgtccgtt acatagcgat tgacgacggt
    22621 gtagacagcg agaaaggcga aagtgagatt gcaccattta agaacatcat caatgaatgg
    22681 gtggcaagag atacaagccg taaagtgaaa tcggcattta agacgaaatt tgcagagggt
    22741 gctcattatg gtgcttatgc tccattagga tataagaaac accctgacat caaaggaaaa
    22801 ctgctggttg atgatgaaac aaaatggatt gttgaaaaaa tcttttccct agcctatcaa
    22861 ggttatggta gtgccaaaat cacaaaggta ctgcgagagg aaaaagttcc gacagcgtct
    22921 tggctgaatt ttacaaggta cggtactttt gcacatatct ttgagggaaa gcccgaaagc
    22981 aaacgttatg agtggacgat tgctcatgtc aaggcaatct taaaaagtga ggtttatata
    23041 ggcaacagcg ttcacaatat gcagtctacg gtttcattca agagcaagaa gaaagtgcgt
    23101 aagcccgaaa gtgaatggtt tcgagtggaa aatacccatg aaccgattat tgacaaagaa
    23161 gtgttctatc gtgtgcagga acagataaaa tcaagacgta gacagacaaa ggaaaaagca
    23221 acaccgatat ttgcagggct tgtcaagtgt gcggattgtg gctggtctat gcggtttgca
    23281 acgaatacgg caaataaaac gccttacagt tattatgctt gcagttacta cgggcagttt
    23341 ggcaaaggta cttgttctat gcactatatc cgttatgatg tgttgtatca agccgtattg
    23401 aaacgattgc agtattgggc taaggcagta cagcaggacg aagaaaaggt gctgaacaag
    23461 atacagaaag caggcaatgc agagcgaata cgggaaaaga agaaaaaggc aagtgccttt
    23521 aagaaagccg agaaccgaca aaatgaaatt gaccgtttgt ttgcgaaaat gtatgaagat
    23581 agagcctgtg agaagataac agagcgaaat tttgtgatgt tgtctagcaa gtaccaaaaa
    23641 gaacaaatag aactggaaca gcagataacc aacctaagag aagaattaag taaaatggaa
    23701 caggatatga taggtgctga aaagtggatt gagttaatca aggagtattc cgtaccaaag
    23761 gaactgacag caccgttatt aaatgcaatg atagaaaaaa tccttattca tgaagcaata
    23821 acgaatgagg ataacgaaag aatacaggaa atagagatat attaccgatt tattggaaaa
    23881 gtagactgat aaagagggtt catatcttta actaagggaa actggagaat ctattcctga
    23941 tataggtagt tgcattaaac ttgcaaaatt gtacaaggtc aaactagatg acttagttaa
    24001 tcattcggaa gaaaaaacag gtattatagt acctcctaaa ggaaagtttt tctttggagc
    24061 agtagtagtt ggtgaacgtg gtcagattgt cattccaaaa gaagcaagag aagtatttaa
    24121 tataaatgca ggagataaat tattggtact tggagatgag gaaagaggat taggaattgt
    24181 tcatcaacgt gatttgataa attttatagg tcatatggga gtagtacgta aagaagaatg
    24241 attttaaaaa attataaaag gaggtatgaa atgtgagtaa aatagtattt tttagcattc
    24301 ttgcatatgg tcatactaat cctactattg aagtagttcg tgaattagtg gatagaggaa
    24361 atgaggtatt gtattattct tttaatgaat ttaaagataa aattgaaggt gctggagcaa
    24421 aatttatttg ttgtgataaa taccttccag aattactccc tggagatgag aaaaaaattg
    24481 gtaaggattt tcctggatta attgagatga tagtagatac tactatttct ttagatgaaa
    24541 aagtttgtag ggaacttaaa gattttaatc cagattgcat tgtatcagat tcattaagtt
    24601 tttggggaaa gttatttgca caaaaactta atattccata tgtatgctct acaacaactt
    24661 ttgcatttaa taaacataca gcaaagttga tgaaacaaaa ctttatagaa attatacgta
    24721 tgatttttgg tgtaagacgc attaataaaa aaataaagct tttacaaaga aaagggtatg
    24781 aagtaaaaaa ttttatttct atagtttcta atgataatga tacggatacc attgtatata
    24841 catcaaaaga atttcaacca atggtagaaa ccttctctag taagtattca tttgtaggtc
    24901 catcagtatc aaagttgatt attaaaccta aagagagaaa aagaaagtta atatatattt
    24961 cgcttggtac aattaataat aaaaatgtta gcttttataa aaactgtata aatgcattta
    25021 aagattctga tgttgatgtg attatgtctg ttggtagaag tactgatatt aaaagtttag
    25081 gaaatatacc aaataatttt gaggttaaaa atagtgttga acagattgct atacttcaaa
    25141 aaactgatgt gtttattact cattctggga tgaatagtgt taatgaaagt ttatattatg
    25201 gagtacccat ggtattattt cctcaacatt cggagcagag aatggtagct aagagagttg
    25261 ttcatttggg agcaggaatt atgttaaaag aggataagtc cgaaagtatt aaaaaagcag
    25321 tatttcaagt tataaatgac aatgaatata aagaaaatgc aactaaacta tcaaaaagct
    25381 tttataatgc aggaggctca aaaaaagcag cagatgtaat attacagatt atatgtaata
    25441 gtagacaagt tgacatataa tagatttgtt aaaaatatat atacatacat gcaatttaat
    25501 caaagtactg acagtggggt cttttaacct accagttggg gcatatatat tgatgctaaa
    25561 tatgagggat gttatatttt aataaaaaag ataacatcct ttttttagtg cgttatatca
    25621 agttttaaat aataaatgaa ggaggattaa tatgaaaaaa tataaagtta tttttatagt
    25681 attaattata ggtattataa gtgtaacagg gtttgttgta tataaaaact gcattaaacc
    25741 atggaaagat gctgaaacat atattgataa gtatatggta aaacaagata tttcaaaaga
    25801 taatataaaa agtattacaa aagaaaaagc caagaaggca agttacgaag gaatactata
    25861 caaagtgtat tacaaagatg accctcgata tgagtatcaa tatttttata gtgataatta
    25921 tcatgctcta tatgtaaata aagtaatgtt acaaatttat gatgtggaaa ataataataa
    25981 acttttaaga agtaatgagg aattaaaaag tgtaaagtat ccaccaatat atcttaaata
    26041 taagccagat aaatgattta tgacaaaata gaatgatttt tattaaaa
//
LOCUS       DAMJWP010000033        25977 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.034, whole genome shotgun sequence.
ACCESSION   DAMJWP010000033 DAMJWP010000000
VERSION     DAMJWP010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25977
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.034"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            176..3724
                     /locus_tag="PC700_001185"
     CDS             176..3724
                     /locus_tag="PC700_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2580501.1"
                     /translation="MMNRLKPRILGISILFIIIIILTCGAVFKFKFKLDNKLKETTHT
                     YLSEFTEHNIINLKTRLSGQFDMLESIANFIGNLNNINNEMVIALMDSDVKRSSLIRM
                     TISTLDGECYSNDGITHNVQDRDYFKKAKNGEKNISEPLKSIVNNKEIIVLSTPVYKS
                     GKIVGVLSGIYDSKKLNELLGLSAFDEKADVFVAKKNGDIVAQKTNKNRKTISCIFQL
                     FDKDNCDEDDCLKTIKENMKNDKSGYFNYNLDNEKFMLNYQPLGVNDWYVFSVVPNKI
                     VSIQSENISYDVYIFALEILLVILIFIIYIACIINNNTKIIINEKEKFKVLTDNILGG
                     VKTCLKDEYMTMTYVSQGFLELTGYSCEDLNKIFKNRFKDMIYKDDLDKFNCIILNQL
                     EKQDKIEVEYRIVRKDGSIIWILERSKSINDNKGSECIQGVLTDITELKKIQEELDSK
                     RFEIQAINDSIVGGIVITEINDDFDSIYLNEGYLNMIGYTRKQLEDELNNKLIKLIFK
                     DDRTELINSINKQLKLSDTFASEQRVEKRDGTIIWILLKGRKIIYDNGKSVLCCVIVD
                     ITSAKLLEQELKKSEERHEIILKQTQNIIIEWDIEKDKMNISPNWYNKFKYDPITENA
                     TKNIIKSVDIYHDDMDKFISLIKLTSQGSEYEECEIRLRTRDTIYAWFSIKATTLFDN
                     NGKPYKVVGSLTDIDESKREAEKLKERAEKDPLTDLYNKKASQSLIEEYITLDKKRKG
                     ALMIIDIDNFKGINDNLGHLYGDAVLSEIANDLINLFRATDIVGRIGGDEFIVFIKDI
                     SETRDIINKAEELVKTFERSFLGKGRNYKISLSVGIAKFPQDGMDYMTLFRNADMALY
                     SAKGKGKNCYTLYDKKIDKVQYITNTRNAGDSDNVEMSFKEHITNYIFDILYESSDIE
                     VAVNLILSIVGKHFNVSRMYIFENSDDNRVCKNTFEWCNEGVTSEINNLKNICYKDLG
                     NYLLNFNADGLFYCNDIENLGSDVKKVLKPQGIRSILQCKILEGNEFRGFIGFDECSY
                     NRMWTNDEVNTLTFISKTLSMFLLKMRIQSKLEESLTIQKSVMDNMDIWAYVVDRNTY
                     ELLFINKKMLESASNTHVGDLCYKAIWNNQDKPCKKCPMIYLKDGDDTYKSFMRNKKF
                     EVQANITTTTLKWFDGKDACLIFGSEVTSIEAEENT"
     gene            3909..5843
                     /locus_tag="PC700_001186"
     CDS             3909..5843
                     /locus_tag="PC700_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HDF2580502.1"
                     /translation="MENKFLPISKQDMIDRGWEELDFVLVTGDAYVDHHSFGTAIISR
                     VLENAGYKVGIIAQPDWRTTDDFMKLGKPRLAFLVNAGNMDSMVNHYSVSKKHREKDM
                     YSPGGKMGYRPDRATIVYCNKIREAYSDVAIVIGGIEASLRRFAHYDYWSDKVRKSML
                     IDSGADLLVYGMSEKQIVEVANALNDGYDPKYIRHIDGTCYISDTLEEIYDKYILIPS
                     YKEICEDKMKYVEAFKIQYDEQDPFRGNIIVQPHGSKYLVQNKPEKPLSREELDEVYG
                     LPYQKTYHPVYEKFGGIPAIEEVKFSIVSSRGCFGSCSFCAITFHQGRAVQSRSEKSI
                     IDEAIGITNLDDFKGYIHDVGGPTANFRRPACKKQITKGACKNRQCLSPSPCKNLDAD
                     HSEYLHLLRAVRKLPKIKKVFVRSGIRYDYVMADKNNKFLRELIEHHVSGQLKVAPEH
                     ISEEVLKYMQKPAGKTYDKFRQKFFAINEELGKKQYLIPYLMSSHPGSTLNSAIELAE
                     YLRDTHYQPEQVQDFYPTPGTLSTTMFYTGIDPLTMKPVYVPKSKRDKAMQRALLQYR
                     APRNYDLVYSALVEAGREDLIGFGHRCLIKPKNEKPYFNRNNSKKNVSKGTNKNKKTN
                     TNNRSNKNQQKSSTKKKKKH"
     gene            5949..6944
                     /locus_tag="PC700_001187"
     CDS             5949..6944
                     /locus_tag="PC700_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2580503.1"
                     /translation="MKKYISIILLVVVTMVVLVGCSPGKDNPKNKELSVVKQVKVAVP
                     DGLPAVAIAKLANENPEIKEGYETVYSIEKTPEAISTRVMKKDADIAIVPSNMAAIAY
                     NKTSSYNIVGTVGMGSLYLVSTENIKDYSDLVGKEVGCTGKGLTPDITIKSLLLQKDI
                     NYSNIKFNYVNSASELVPLLATGKVKTGIVPEPALSALMSKNPDIKIIKSLNDSWKEV
                     SGSKDGYPQSTLIVKSEFLRDNKDFVDSFVGQLSNSIDWANKNPEELGAYSEKIKIST
                     ESKIIGKSLERANLKYIPVKDMIKDYKNYYEKLANFDDKTLGGKVPDEAIYFVEK"
     gene            6916..7701
                     /locus_tag="PC700_001188"
     CDS             6916..7701
                     /locus_tag="PC700_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDF2580504.1"
                     /translation="MKQYTLLRNKKNKEQFIDKMEVTLSCVILLFLWQIIALKIDNDI
                     FLPTVGQVFNSIKEIMLNSSFYIDILYSMGRCFFSFLLAMIFAIILSIVSYLNRFFRN
                     LLKPINALTRSIPTMILVVLALIWFEKDSTPFIVGFTIVFPILYDNVLGAILNIDKNL
                     LEMANVYKIRFIDKVLKIYLPAIKFQIISILVSTFSLSLKVAIAGEVYSQPTYGIGTM
                     IQTEKINFNTSGIFAWIIIVLLISAILQIAQKFLARRAFLWKR"
     gene            7689..8387
                     /locus_tag="PC700_001189"
     CDS             7689..8387
                     /locus_tag="PC700_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2580505.1"
                     /translation="MEKIKLSIENINKRYDSRIIFRDFNIDFYVNEVNCILGKSGCGK
                     TTLLNIISGIIKNDTNNLNIKENLNRVGNKLEASYIFQDDRLIDWLTVEENIKIVVNK
                     YYNKTQLNKICDEYLELVGISDYKKFYPQMLSGGIRQRVNIARAFIYPSKNIIMDEPF
                     KSIDAKNTQLIMDNFRNILRKEKRTVLFVTHNIEEALFLADRIFILGDSPIRIKKILK
                     NSKELEKNEVLKLI"
     gene            8510..8983
                     /gene="ybaK"
                     /locus_tag="PC700_001190"
     CDS             8510..8983
                     /gene="ybaK"
                     /locus_tag="PC700_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HDF2580506.1"
                     /translation="MVKTNAMRILDSNKIDYKVMSYEVKSEHVDGVEVAHDIGRDVNE
                     VYKTLVTQGVSKNIYVYVIPVHENLDLKKAAKVAKEKSVEMIHVKDINKLTGYIRGGC
                     SPIGMKKLYKTFVNESAKNLDTIIVSAGKIGYQIELSPFDLQRLIKVEFVDVIKK"
     gene            9180..10019
                     /locus_tag="PC700_001191"
     CDS             9180..10019
                     /locus_tag="PC700_001191"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HDF2580507.1"
                     /translation="MRKIKSEQIVEQVKKLCIEASLYLGEDVLSCIKEKAKSEKSEVG
                     KNILNILVENAEIAKEKNIPICQDTGMAVFFVEIGQEVLIEGDTLTDAINEGVRQGYE
                     EGYLRKSVVSPINRVNTKDNTPAVIHYDMVKGDKIKIEFAAKGFGSENMSKMKMLKPS
                     DGLEGIKKFIIDTVSEAGPNPCPPMVIGVGIGGTVDKCAQIAKKALFRELGEFNKDEN
                     IAKLESELLTAINKLGIGPQGLGGTTTALGLNIETFPTHIAGLPVVVNINCHASRHKK
                     VVI"
     gene            10032..10580
                     /locus_tag="PC700_001192"
     CDS             10032..10580
                     /locus_tag="PC700_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HDF2580508.1"
                     /translation="MIKITTPVNEIDIAKLNCGDTISLSGILYTARDAAHKRLIDCIN
                     KGEELPFDVYGQGIYYVGPTPTKPGEVIGAAGPTTSYRMDDLTIPLLERGLRLMIGKG
                     KRSDEVIEGMQKYGAVYLAAIGGAGAYISNSIKSCEIIAYEDLGAEAIRKIVVEDLKL
                     TVAIDSYGNNIYEQGRAIYECK"
     gene            10646..11827
                     /locus_tag="PC700_001193"
     CDS             10646..11827
                     /locus_tag="PC700_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HDF2580509.1"
                     /translation="MSKDYAKLALEMHEINKGKVSVESKVEVKTKDDLSTAYTPGVAE
                     PCLKIHENQDDVYIYTSKGNLVAVVSDGSAVLGLGNIGAEASIPVMEGKAILFKQFAD
                     VDAFPICLKTNDVDEIVKSVELMEPVFGGINLEDISSPRCFEIEERLKKSLSIPVFHD
                     DQHGTAIIVAAAIINSIKLIENKKIEDLEIVINGAGAAGIAIAKILLNMNVKNIILCD
                     RSGALEASIENLNYVQKEMLKVTNIRNEKGPLKDIIKGKDVFIGVSGPGAVTKEMVES
                     MSEKPIILAMANPTPEIMPEEAKLGGAFIMGTGRSDFPNQVNNVLAFPGIFRGALDVK
                     AKEINEEMKIAAAYAIANTISDEEICPEYILPDVFNKNVVKNVAKAVKEAAIKTKVNR
                     I"
     gene            12000..12263
                     /locus_tag="PC700_001194"
     CDS             12000..12263
                     /locus_tag="PC700_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2580510.1"
                     /translation="MEETKFCQCCAMPMGQTDELYGTNKDGSKSTDYCSYCFKDGEFT
                     ADISMEEMIEVCIPHMLQSNKDMTEDEARTMMNNFFPTLKRWK"
     gene            12789..13472
                     /locus_tag="PC700_001195"
     CDS             12789..13472
                     /locus_tag="PC700_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2580511.1"
                     /translation="MSSILLVEDDLSLIYGLEFSIQKSGFSIDIARTVKEAMQMYEEK
                     NYDLLLLDVSLPDGNGFQICEKVREVSNIPIIFLTASDEEVNVVMGLDMGGDDYITKP
                     FKLNELISRIKALLRRYNVASNVNELSSNNITIKLLENRVFKGEFEVELTTAEYKLLC
                     LFMKNKNIVLTRKNILDKLWDGNGSFIDDNTLSVYVRRLRNKIEDNPENPKFLLTVRR
                     MGYKWNVIK"
     gene            13483..14724
                     /locus_tag="PC700_001196"
     CDS             13483..14724
                     /locus_tag="PC700_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2580512.1"
                     /translation="MKIFSNKDIKIFFLILISILVMFIIVGQIVVISITNNYKKTLIE
                     HDYNIAGYLYSIGVDKSKIPSAFVAHEDKYILKGKEILDTAGYGFDIDVSFTPSVKLF
                     QSKYIKTMSIYLLVFFTMIIFILYMYFFRQQSKIEEASYKIDLFMNGNINERLNTYEE
                     GSLSKLFSSIDVMSTSLNTHILKEKQSREFLKNTISDISHQLKTPLTALIMYNQILQE
                     ESHNSDVVEAFVQKSQSELERIETLIKNLLKITKIDSKTIVLNKNNVNLKNLIVKILY
                     SFETRAEKEGKVISINGSENTLLFCDYEWMSEALSNLIKNALDNTKENDKITIEWFET
                     TITTIVSVKDTGNGIHVEDIHHIFKRFYRSKFSKDNQGLGLGLPLVKSIVEQHNGTIS
                     VESKLSQGSTFTISFLKLTNM"
     gene            14796..15479
                     /locus_tag="PC700_001197"
     CDS             14796..15479
                     /locus_tag="PC700_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2580513.1"
                     /translation="MDILVVNNLCKTYGNGYTKVEALKDVSFTVSKGEFVTVVGESGS
                     GKSTLLNLLGGLDTPTSGRIFIDGKEICSMKEEKQTIFRRRNIGFIFQAYNLIPELTV
                     EQNIIFPLLLDYKKPDTIYLDEVLTILGLKDRKNHLPNQLSGGQQQRVAIGRALITKP
                     SIILADEPTGNLDSKNSNDVISLLKVTAEKYNQTIIMITHNTNIASTADRILQVSDGV
                     LTDLGVNRE"
     gene            15476..17806
                     /locus_tag="PC700_001198"
     CDS             15476..17806
                     /locus_tag="PC700_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2580514.1"
                     /translation="MRSYLELVPQYAKVHKKKNRTTILCIIIAVCLVTAIFGLADMAI
                     QCQKIQFIKDDGNWHIVIRNIDEKFISEINSRVDIAHHGRGQVVDSGEFKSKEILIQG
                     LDEEVAKQFNLEIIRGRYPIGENETLIDEDAVKSFNLKIGDFIKIKLADGRNKNYKIV
                     GEYSSISSLRATDKHGLILSILGTQAIKKSETYDYYYITFKEKVNIRKATSDIKNTFN
                     LSDKQIGKNERLLSIIGQGDSDFSKPLYLTAGLLFVLVLVASIIMIYNSFNMSVIERV
                     KFFGLLRCLGSSKHQVKKFVVLESLILSIKAIPIGLVLGSLVTIISSIFLKYVNELFS
                     SMPILKVSFIGIAFGIIVGFLTVILSAIIPAKKASRVSPLSAVRGNLMSKEILNNGKT
                     SKVANTGSFKIETSMGLNHAFSRKKSFVLMTSSFAISIILFLGFSVLIDLMYQGLKPM
                     KPEAPHISIVDPNFKSVLTKEDINEISNIKGVKKVYSRMMTDIEATYNGKKTKSQLIS
                     YEKYQFNWAKKYLLKGEVNENKLQSENLALVEEGHGFDIGDKIYIKNRNAEKELEVGG
                     ILSSIPFEAKDKYIGNIITSENTFTNITGIKGYIIMDIQVDKDTPGDLIENIRRVVDD
                     NLEVRDKRQSNLEVKNSFYTMATFVYGFVFIIAIISMFNIINSMNISVTSRKNYYGVM
                     RAVGISNRQLRRMVIVEAGSYAMSGCLLGSILGLMLHRYMFISLVSSMFHLEWQVPFN
                     LLLITVLTMIIITLLSVRKPVKKICELDIIDSINAQ"
     gene            complement(17987..18829)
                     /locus_tag="PC700_001199"
     CDS             complement(17987..18829)
                     /locus_tag="PC700_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF2580515.1"
                     /translation="MINIGDFNKLTVKRKTEFGYFLDGQTNNTKDDILLHNRLIGKNE
                     INIGDEVNAFIFKDSDDRTAATLIPPLAKVGDVAHLKVVDNTDIGTFIDMGLPKDILV
                     PFKAKTYPLFRDEKYLFYIYLDKSKRIAATTDIDSYLLTDHTYNVGDIVTGVVYGFQT
                     NNSAMICVDNKYAGVILHNEYFTELKAGDVLENLHVIKIYEDGKLGLSPRGNRKDELD
                     TLENKILSYLEGSDGYMRFNDKSDPKDISILFNSSKKNFKRALGVLMKKGLIYQDEEG
                     TYLK"
     gene            19124..19858
                     /locus_tag="PC700_001200"
     CDS             19124..19858
                     /locus_tag="PC700_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2580516.1"
                     /translation="MKIVNKSSNIPLHTQLSSIIREMIETGELKEGDAIMPERELCNI
                     QNVSRMTVNKTIVGLVTEGLLYRVQGKGTFVAKQKKKYQFSNVKGFTDVMKEKGVNIK
                     TDILSFEMELPDDLVKRKLGISDNTTNIYKIVRLRYTDGEPFGLEIVYLSEEMCKGLT
                     KGILDNSSLYRVLNEKYGYKIQKAEQVMEPVILSDEESKLLETDEGALALKLHRNSYN
                     REGSPIEYTISIFRTDKYQYEIVLSE"
     gene            19868..21001
                     /gene="nagA"
                     /locus_tag="PC700_001201"
     CDS             19868..21001
                     /gene="nagA"
                     /locus_tag="PC700_001201"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDF2580517.1"
                     /translation="MKCLINGKIILKNQILENKVLVFDEKIIDIADSVPKDCEVIDAD
                     GKYISPGLIDIHIHGNMGKDTMDSTDESIETISKSIMRHGVTSFLPTTMTMDKEHVYD
                     ALEVIKKAQNRKLEGAQVLGAHLEGPFINENYKGAQNEKFIINSKYEFIKEYKDVIKV
                     ITYAPEKDIDFDFTREIKRCTDIVLSIGHSNANYDQAKEAINLGVTNITHMFNAMTGL
                     NHRDPGVVGAALTTNVYSELIADTIHINKDLFQFILNNKGKERLILITDSIEAGGLED
                     GNYSLGGQKVIVKGNEARLENGALAGSVLSLNKMVFNFLDNTNLKVNEAINLASLNPA
                     TSLGINDKKGSLEIGKDADIAVFDENLDCKMTLCLGDVVYKNI"
     gene            21100..21849
                     /gene="nagB"
                     /locus_tag="PC700_001202"
     CDS             21100..21849
                     /gene="nagB"
                     /locus_tag="PC700_001202"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDF2580518.1"
                     /translation="MRVLVCKDYDGMSKKAAEMIAAQIVLKPNSILGLATGSTPVGMY
                     RDLVKKYNDNIVDFSDVMSFNLDEYYKLPISNDQSYDYFMKENLFNHVNIKLENTHLP
                     NGMADDIEKECLNYEASIDAAGGIDVQVLGIGRNAHIGFNEPDTKFAKRTHVVELTES
                     TIEANARFFKSREDVPKKAVSMGIGSILKSKKILLLASGEEKADAVYNTVYGDITPEV
                     PGSILQLHKDTIVIVDEAAASKLNPKDYKLV"
     gene            22024..22296
                     /locus_tag="PC700_001203"
     CDS             22024..22296
                     /locus_tag="PC700_001203"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580519.1"
                     /translation="MLKIIMFSGILSSIIKLPIIGILPIGNIKKNTGISDSKKDGKQR
                     RSNLGISIYQLPSFLYSLINNSVPSNKPIPPEEIKLIKMYLLKSCI"
     gene            22956..23660
                     /locus_tag="PC700_001204"
     CDS             22956..23660
                     /locus_tag="PC700_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2580520.1"
                     /translation="MSKLIYIADDEDNIRNLVKTFLKNEGHDVMDFKTGDELLEQFNI
                     KECDLVILDIMMPGSSGFEVCTKLREKSTVPIIMLTARDTDIDYITGITLGSDDYFTK
                     PFSPMSLVMRVKSIFRRIEFEKKQNYDKYSNSIDMELKFGDVIINKKNKIVTSKNVNI
                     DLTPNEYNLLTYLFENIDRAVSRDELLNKIWGYDIEVETRAADDTVKRLRKKILDTNI
                     LIETVWGFGFRLKEKS"
     gene            23662..25149
                     /locus_tag="PC700_001205"
     CDS             23662..25149
                     /locus_tag="PC700_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2580521.1"
                     /translation="MNNGNDKNRIGKIKSKFKLKFSKKNISIKTRILLSILCLIIIVF
                     MIILVSFNVFLDTYIKTTANEELTKSTEIVEHMDSNFRPIKPPQDDGKLPPKVLSNFM
                     RNVQDKVRMAETQSDADAMVVDSKYNLIFPTREDDFLKNVDEMELIRECIQNEKLDLN
                     SDENTRISTRNKDYYISTVKITSIYGEQDKYLILFIDISKTLNLAQKINIVLISVMCF
                     AGILAIFTAVILSEKIAKPIKELCEFAKKMGQGDFKRTYFDFSDKELVELSTVMNKSA
                     EYLDKYDNEQKIFFQNASHELRTPLMSIKGYAEAIKYNVIDSKHASDIILEESDRLSD
                     MVEDLLYISKIDNITKDFELVECDLREVFSNCGARQNVRAINKGIKFIYEFDEEMVLF
                     ECDEKNISKAFMNLIENALRYAKSEIKIVCKYNQKNIVVIIEDDGIGIKKEDLPHVFE
                     RFYKGVGGNHGIGLSIVKSIVNKHGGRIYVENGKKGAKFTILFKL"
     gene            25354..>25977
                     /locus_tag="PC700_001206"
     CDS             25354..>25977
                     /locus_tag="PC700_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2580522.1"
                     /translation="MKEQLIFNITNLVTVVFEAFVIHMFLSDFLGQKEGYIKVVRYAK
                     LGFIICLGFCNLITLNPKITMPLIFILIFSTSFLYKGNLKTRLFTTVLLSIFFILSEI
                     VVTSIFVLFVKEGFEIMLENNSIRVLATILSKIVFLLTCKIICLFKKDVHLDMPIKYW
                     LPLFLIPIFSLFLSVSIFDVSKFFSLESLKFLSLISSVGILYINFIVF"
BASE COUNT        10358 a         2677 c         4523 g         8419 t
ORIGIN      
        1 tgatgaaata tgaataaaga ttttacaaat aaatcttaag ctatcttatt taattctaat
       61 gcaggaagaa ggtaaatgta tattgaaaaa atcatagaaa tagaagtaaa caacttaaac
      121 aaatacaaag gtttgcaaaa gaagtaatat aattatatac aatgaggatg tgagtatgat
      181 gaacagacta aagccaagaa ttttaggtat tagtatcttg tttattatta taattatttt
      241 aacatgtgga gctgttttca agtttaaatt taaattagat aataaattaa aagaaaccac
      301 acatacatat ttgtctgagt ttacagaaca caatataatt aatctcaaga caagacttag
      361 tggtcaattt gacatgcttg aatctattgc taacttcata ggaaacttga ataatataaa
      421 taatgaaatg gtgattgctt taatggattc tgatgtaaag cgaagttctt taattagaat
      481 gactatctct acattagatg gggaatgtta ttctaatgat ggaatcaccc ataatgttca
      541 agatagagat tattttaaaa aagctaaaaa tggagaaaag aatatttcag agcctttaaa
      601 atctattgtt aataataaag aaataatagt tttatctacc ccagtttata aaagtgggaa
      661 aatagttggt gttttaagtg gaatttatga ttcaaaaaag ttaaatgagt tattaggatt
      721 atctgcattt gatgaaaaag cagatgtttt tgttgcaaag aaaaatggag atatagttgc
      781 tcaaaaaaca aataaaaata gaaagacgat atcatgtata tttcagcttt ttgacaaaga
      841 taattgtgat gaagatgatt gtctaaaaac tataaaagaa aatatgaaaa atgataagtc
      901 tggatatttt aattataact tagataatga aaaatttatg ttaaactatc agccacttgg
      961 agttaatgat tggtatgttt tttctgttgt gccaaataaa atagtgtcta ttcagtcaga
     1021 gaatatatct tatgatgtat atatttttgc attagaaata ctactagtta tattgatttt
     1081 tataatatat atagcatgta ttataaataa taacaccaaa attattataa atgaaaaaga
     1141 aaaatttaaa gtactcactg ataatatatt aggaggggtt aaaacctgtt taaaagatga
     1201 atatatgact atgacatatg taagtcaggg gtttttagag cttacaggtt actcttgtga
     1261 agatttaaat aaaatattta aaaatagatt taaagacatg atttataaag atgatttaga
     1321 caagtttaat tgtattattc taaatcaatt agaaaaacaa gataaaattg aagttgaata
     1381 tagaattgta agaaaagatg gaagtataat ttggattttg gagagaagta agtctataaa
     1441 tgataataaa ggaagtgagt gtattcaggg agttttaact gatataacag aacttaagaa
     1501 aatacaagaa gagttagatt caaaaagatt tgaaatacag gctatcaatg atagtattgt
     1561 tggtggaata gtgataactg agataaatga tgattttgat agtatatatt taaatgaagg
     1621 ttatttaaat atgattggat atacacgcaa acaactggaa gatgagctaa ataataaatt
     1681 aatcaaactt atttttaaag atgatagaac agaattaatt aatagtataa acaaacaact
     1741 caaattaagt gatacatttg catctgaaca aagagttgaa aaaagggatg gtactattat
     1801 atggatttta ttaaaaggaa gaaagataat ttatgataat ggaaaatctg tactttgttg
     1861 tgttattgta gatatcactt ctgctaaatt attagagcaa gaacttaaaa agagtgaaga
     1921 acgtcatgaa atcattctaa agcagacgca aaatatcata atagaatggg atatagaaaa
     1981 agataaaatg aatatatcac ctaattggta taataagttt aaatatgacc ctataactga
     2041 aaacgcaaca aaaaatataa ttaaatcagt agatatatat catgatgata tggataagtt
     2101 tatttcatta attaaactta cttcacaagg tagtgagtat gaagagtgtg aaattagact
     2161 tagaactaga gatactattt acgcttggtt tagtataaag gcaacaactt tatttgataa
     2221 taatggtaaa ccttataaag tagtaggaag tttgactgat attgatgaat caaaacgaga
     2281 agctgaaaag ttgaaagaaa gggcagaaaa agaccctctt acagatttgt ataataaaaa
     2341 agcatcacaa tcacttatag aagagtacat aacattggat aaaaaaagaa aaggtgcttt
     2401 aatgattatt gatattgata actttaaagg aataaatgat aatttaggac atttatatgg
     2461 ggatgctgta ttaagtgaaa ttgcaaatga cctaataaat ctatttagag caaccgatat
     2521 agtaggcaga attggtggag atgagtttat tgtatttata aaagatattt ctgaaacaag
     2581 agatattata aataaagcag aagaattggt aaaaacattt gaaaggtctt ttttaggtaa
     2641 aggacgaaat tataaaatat cattaagtgt aggaattgct aagtttccac aagatggtat
     2701 ggactatatg acactattta gaaatgcgga tatggctctt tactcagcca aagggaaggg
     2761 taagaattgt tatacattgt atgataaaaa aattgataaa gtccaatata taactaatac
     2821 aagaaatgca ggagatagtg ataatgtaga gatgtctttt aaagaacata tcacaaatta
     2881 tatatttgat atactatatg aatcaagtga tatagaagtg gcagtaaatt taattctaag
     2941 tattgtaggt aaacacttta atgtgagtcg catgtatata tttgaaaatt cagatgataa
     3001 tcgagtatgt aaaaatacat ttgaatggtg taacgaaggt gtaacctcgg aaattaataa
     3061 tcttaaaaat atttgttaca aggatttagg caactatctt ttaaatttta atgcagatgg
     3121 gttattttat tgtaatgata ttgaaaattt aggtagtgat gtgaaaaaag tacttaagcc
     3181 acaaggcata cgttcaatat tacaatgtaa aattttagag ggcaatgaat ttagagggtt
     3241 cataggattt gatgaatgtt catataatcg tatgtggact aatgatgaag tgaacacttt
     3301 gacatttata tctaaaacgc tcagtatgtt tttattaaaa atgcgtattc agagtaagct
     3361 agaagagtct ttaacaattc aaaaatctgt gatggataat atggatatat gggcatatgt
     3421 tgttgacagg aatacctatg aattattatt tatcaataaa aaaatgcttg agtcagcatc
     3481 aaacactcat gtaggtgatt tatgttataa agcgatatgg aataatcaag ataaaccttg
     3541 taaaaaatgc cctatgatat atttaaaaga tggtgatgat acttataaat catttatgcg
     3601 caataaaaag tttgaggtac aggcaaatat aactacaact actttgaaat ggtttgatgg
     3661 gaaagatgca tgtttaatat ttggaagtga agtaactagt attgaagctg aagaaaatac
     3721 ttaaaatact gtgttatata tatacttaaa atcgcaattt gaatttatta gacttaaaat
     3781 gtctatataa atcatttaag ttgtggtttt aagtattttt tatgatataa ttaagcgtta
     3841 agaacaaaat taaaagatac ttttgtataa aaaaatattt gttaaatata tattgagcag
     3901 gtgataatat ggaaaataag tttttaccaa taagcaaaca agatatgata gatagaggat
     3961 gggaagaatt agattttgtt ctagttacag gagatgctta tgtggaccat catagttttg
     4021 gaacagcaat aatatctaga gttttagaaa atgctgggta taaagttgga ataatagctc
     4081 aaccagactg gagaactaca gatgatttta tgaagctagg aaaaccgaga cttgcatttc
     4141 tagttaatgc aggaaatatg gattcaatgg tcaatcatta ttctgtaagt aaaaagcata
     4201 gagaaaagga tatgtattct ccaggtggga aaatgggtta tagaccagat agagctacta
     4261 tagtatattg caataaaata agagaagcat acagtgatgt tgcaatagta ataggtggaa
     4321 ttgaagcgag tttaagaaga tttgcacact atgattattg gagtgataag gttagaaagt
     4381 ctatgttaat agatagtgga gcagatttat tagtttatgg tatgagtgaa aagcagattg
     4441 tagaagtagc caatgcactt aatgatggat atgacccaaa atatattaga catattgatg
     4501 ggacatgcta tatttcagat acattggaag aaatatatga taaatatata ttaattccat
     4561 catataaaga aatttgtgaa gataaaatga agtatgtaga agcttttaaa attcaatatg
     4621 atgagcaaga cccatttaga ggtaatataa ttgttcaacc tcatggaagt aagtatctgg
     4681 ttcaaaataa gccagaaaaa ccacttagta gagaagaact agatgaagta tatggattac
     4741 catatcagaa gacataccat cctgtgtatg aaaaatttgg aggaatacca gctatagaag
     4801 aagtaaaatt tagtatagta agttcaagag gctgttttgg tagttgttca ttttgtgcaa
     4861 taacatttca tcaaggtaga gcagtgcaaa gtagaagtga aaaatcaatt attgatgaag
     4921 caataggaat aacaaattta gatgatttta aagggtatat acatgatgtt ggaggtccaa
     4981 cagctaattt tagaagacca gcatgtaaaa aacaaattac taaaggtgct tgtaaaaata
     5041 gacaatgctt atctccatca ccatgtaaaa atttagatgc agaccacagt gaatacttac
     5101 atttacttag agctgtaaga aaattaccta aaataaaaaa ggtgtttgtg cgttctggaa
     5161 ttagatacga ttatgtaatg gcagataaga acaataaatt tcttagagag ttgatagagc
     5221 atcatgtaag tggtcagtta aaggtagccc cagaacatat atcagaagaa gttttaaagt
     5281 atatgcaaaa accagcagga aaaacttatg ataaatttag acagaagttt tttgctataa
     5341 atgaagaatt aggtaaaaag caatatttga taccctattt aatgtctagt caccctggtt
     5401 ctaccttaaa tagcgctata gagttagcag agtatttaag agatactcac taccaaccag
     5461 agcaggttca ggatttttat ccaactccag ggactctatc tacaaccatg ttttatacag
     5521 gtattgaccc gcttacaatg aagccagttt atgttcctaa atcaaaacgt gacaaggcaa
     5581 tgcaaagggc tttattacaa tatagagcac ctagaaatta tgatttggta tactctgcgt
     5641 tagttgaagc aggaagagaa gacttaattg gttttggaca tagatgtctt ataaagccaa
     5701 aaaatgaaaa gccttatttt aatagaaata attctaaaaa aaatgtatct aaaggaacaa
     5761 ataaaaacaa aaaaactaat acaaataaca gaagtaataa aaatcaacaa aaaagttcaa
     5821 ctaaaaaaaa gaaaaaacat taaagtataa ataaaaatac ttaaaaaaga aggattattc
     5881 gataaattaa cagaaattag tataaataaa tataattatt ttaatgacgt tacctttggg
     5941 aggaagagat gaaaaagtat atttctataa tattattagt ggtggtgact atggttgttt
     6001 tggtaggatg ttcaccagga aaagataacc ctaaaaataa ggagctatca gttgtcaaac
     6061 aagtaaaagt agctgttcca gatggactac cagcagtggc aatagctaaa cttgcaaatg
     6121 aaaatccaga gattaaagaa ggatatgaaa cagtgtatag cattgaaaag acacctgagg
     6181 cgatttccac tagagtgatg aaaaaagatg cagatatagc tatagttcct tcaaacatgg
     6241 ctgcaatagc gtataataaa acttcatcat ataatattgt tggaactgta ggaatgggtt
     6301 cactttattt ggtgtcaaca gaaaatataa aagattattc tgatttagtt ggtaaagaag
     6361 ttggatgtac tggaaaagga cttactccag acataactat aaaaagtctt ttgttacaaa
     6421 aggatataaa ttattcaaat ataaaattta attatgtaaa ttcagcaagt gaactggtac
     6481 cattattggc tactggtaaa gttaagacag gtattgttcc agaacctgca cttagtgctt
     6541 tgatgtctaa aaatccagac ataaaaatta taaaaagttt aaatgactca tggaaggaag
     6601 taagtggttc aaaagacggt tatcctcaat ctactttaat tgtaaagtct gaatttttaa
     6661 gagataataa agattttgtt gattcttttg taggtcaatt atcaaatagt atagattggg
     6721 caaacaaaaa tcctgaagaa ttaggtgcat attctgaaaa aataaaaata tctactgaat
     6781 caaagataat aggaaaatct cttgaaagag ctaatttaaa atatattcca gttaaggaca
     6841 tgattaaaga ttataaaaat tactatgaga aattagctaa ttttgatgat aagacattag
     6901 gaggcaaagt accagatgaa gcaatatact ttgttgagaa ataaaaaaaa taaagaacaa
     6961 tttatagata aaatggaggt aacactttct tgtgttatcc tcttattttt atggcaaatt
     7021 attgctttaa aaattgataa tgatattttt ttaccaacag taggtcaagt atttaatagt
     7081 ataaaagaga ttatgctaaa ttctagtttc tatatagata tactttactc tatggggaga
     7141 tgttttttta gttttttact ggctatgatt tttgccatta tactttcaat agtatcttat
     7201 ttaaatagat tttttagaaa cttattaaag ccaatcaatg ctttgacacg ttcaatacca
     7261 actatgatat tggtagtact tgcacttatt tggtttgaaa aggatagtac cccttttata
     7321 gttggattta ctatagtttt tcctatattg tatgataacg ttttgggcgc tatattgaat
     7381 atagacaaga acttattaga aatggctaat gtgtataaaa taaggtttat agataaggtg
     7441 ttaaaaattt atttaccagc cataaaattt caaattatat ctatactagt atcaacattc
     7501 tctttaagtt tgaaagttgc tatagcagga gaggtatata gtcaacctac atatggtatt
     7561 ggaactatga tacagactga aaaaattaac tttaatacat ctggaatatt tgcatggata
     7621 attattgttc ttttaatatc tgctatttta caaatagccc aaaagttttt agcaaggaga
     7681 gcttttttat ggaaaagata aagttgagta ttgaaaatat aaataaaaga tatgatagtc
     7741 gtattatatt tagagatttt aatattgatt tttatgtaaa tgaggtaaat tgtatccttg
     7801 gaaaatctgg ttgtggaaaa actacactct tgaacataat aagtgggatt attaaaaatg
     7861 acaccaacaa tctaaatatc aaagaaaatt taaatagagt aggaaataag cttgaagcta
     7921 gttacatatt tcaagatgat agacttatag actggttaac agtagaagaa aatataaaga
     7981 tagttgtaaa taaatactat aataaaactc aattaaataa aatttgtgat gaatacttgg
     8041 aattggttgg aattagtgat tataaaaaat tctatcctca gatgcttagt ggaggtatta
     8101 gacaaagagt aaatattgct agagctttta tatatccatc aaaaaacatt ataatggatg
     8161 aaccttttaa atctattgat gcaaagaata cacagcttat aatggataat ttcagaaata
     8221 tattaagaaa agaaaagaga actgtattat ttgtaactca taatattgag gaagcattgt
     8281 ttttagcaga taggatattt attttaggag actctccaat tagaataaaa aaaatattaa
     8341 aaaattctaa ggaattggaa aaaaatgaag ttttgaagtt gatatagagt tataataata
     8401 ctgtcgtttt aaaatatatt tggttaaaat ctataagact atattaaaat agtacagaat
     8461 attcaaaact gtgtataatg ttcgtatttt ataaaaagga agtgaatgta tggttaagac
     8521 taatgctatg agaatattag attcaaataa gattgattat aaagtaatgt cttatgaagt
     8581 gaaaagtgaa catgtggatg gtgttgaggt ggcacatgat ataggaagag atgtaaatga
     8641 agtgtataag acattagtaa ctcaaggagt tagtaagaat atatatgtat atgtaatacc
     8701 agtacatgaa aacttagact taaaaaaggc tgcaaaagta gctaaagaga aaagtgtaga
     8761 gatgattcat gtaaaagata taaataagct tacaggttat attagaggcg gatgttctcc
     8821 tataggtatg aaaaagcttt ataaaacttt tgtaaatgaa agtgcaaaga atttagatac
     8881 aattattgtg agtgcaggaa aaataggata tcaaatagaa ctatctccat ttgatttaca
     8941 aagacttata aaagtagaat ttgtagatgt aataaaaaaa taagaattac atataaaata
     9001 tagagcttat attcttaata ataggtgttg taatattaat aaaatgaata ataattttaa
     9061 aataaatatt cagttactta tgtgtaattg gtataaaaat attgagaaat tttaaaaaca
     9121 atggtaaagt gtaagtataa tcacgaatat tttttataaa gtaatggagg gatgttcgct
     9181 tgagaaaaat aaaatctgaa caaatcgtcg agcaggtaaa aaaactttgt atagaagcga
     9241 gcctatattt aggagaagat gttttaagtt gtataaaaga aaaagcgaaa agcgaaaaga
     9301 gtgaagttgg aaaaaatata ttgaatatat tagttgaaaa tgctgaaata gctaaagaaa
     9361 aaaatatacc aatatgccaa gatactggta tggctgtatt ttttgttgaa ataggacaag
     9421 aagttttaat tgaaggagac acacttactg atgctataaa tgagggagta agacaagggt
     9481 atgaagaagg ctacttaaga aaatctgttg taagtccaat taacagagta aatacaaaag
     9541 ataatacacc tgctgttatt cattatgata tggtaaaagg tgataaaatt aaaatagaat
     9601 ttgcagctaa gggatttgga agcgaaaata tgagtaagat gaaaatgtta aagccatcag
     9661 atggattgga aggaattaaa aaattcataa tagatacagt ttcagaagct ggacctaacc
     9721 catgccctcc tatggtaatt ggagttggta taggtgggac tgtggacaaa tgtgctcaaa
     9781 tagctaaaaa ggcacttttt agagaactag gtgaatttaa taaggacgaa aatatagcaa
     9841 aacttgaaag tgaactatta acagctatta ataaacttgg tataggacct caaggcttag
     9901 gtggtactac aacagctttg ggattaaaca tagagacttt tcctacacac atagcaggtc
     9961 tacctgttgt tgtaaatata aactgccatg catcaagaca taagaaggta gtaatttagg
    10021 aggaaaataa aatgataaaa ataactacac ctgtaaatga aattgatata gcaaaattaa
    10081 attgtggaga tacaattagt ttgagtggta tactttatac agcaagagat gctgctcata
    10141 aaagattgat agattgtata aataaaggtg aagaattacc atttgacgta tatggtcaag
    10201 gaatatatta tgtaggacct actcctacaa agccaggaga agttataggt gctgcaggtc
    10261 caacgacgag ttatagaatg gatgatttga ctatacctct tttagaaaga ggtcttagat
    10321 tgatgatagg caagggaaaa agaagtgatg aagttataga aggtatgcaa aaatacggag
    10381 cagtttatct tgctgcaata ggaggagctg gagcgtatat ttcaaattca ataaaatcat
    10441 gtgaaattat tgcatatgaa gatttaggag ctgaagctat aagaaagatt gtagtagaag
    10501 accttaaact tacagtagca atagatagct atggaaataa tatatatgag caaggtcgag
    10561 caatttatga atgtaaataa tgtttagaaa taaatatgaa tatcaaaatt aaaaatagtt
    10621 gtgtatattt taaggaggaa aattgatgtc taaggattat gcaaaattgg cattagaaat
    10681 gcatgaaatc aacaaaggga aagtaagtgt agaaagcaag gtagaagtta aaactaagga
    10741 tgatttatca acagcatata caccaggggt tgctgaacct tgtttaaaaa tacatgaaaa
    10801 tcaagatgat gtttatatat acacttctaa agggaatcta gtagcagtag ttagtgatgg
    10861 aagtgctgta cttggattag gtaacattgg tgctgaagca tctatacctg ttatggaagg
    10921 taaagctata ttatttaaac aatttgcaga tgtagacgct tttccaatat gtttaaagac
    10981 taatgatgta gatgagatag ttaaatctgt agagttgatg gagcctgtat ttggaggtat
    11041 aaatttggaa gacatatctt ctccaagatg ttttgagata gaagagagat taaaaaaatc
    11101 tttaagtata cctgtttttc atgatgacca acatgggaca gcaataatag ttgcagcagc
    11161 cataataaac tcaataaagt taattgaaaa taaaaaaata gaagatttag aaatagttat
    11221 aaacggagca ggagctgctg gtatagccat agcaaagata ctacttaata tgaatgttaa
    11281 aaatataatc ttatgtgata gaagtggagc attggaagct agtatagaaa atctaaacta
    11341 tgttcaaaaa gaaatgttaa aagtaacaaa tataagaaat gaaaaaggac ctttaaaaga
    11401 tattataaaa ggaaaagatg tatttattgg tgtttcaggt ccaggagctg ttacaaagga
    11461 aatggttgaa agtatgtctg agaagccaat aatattggca atggcaaatc ctactccaga
    11521 gataatgcca gaagaagcaa aattaggtgg agcatttata atgggaacag gtcgttcaga
    11581 cttccctaat caagtcaata atgtactggc ttttcctgga atatttagag gagcattaga
    11641 tgttaaggct aaagagataa atgaagaaat gaagatagct gctgcatatg caattgctaa
    11701 tacaataagt gatgaagaaa tatgcccaga gtatatatta ccagatgtat ttaataagaa
    11761 tgtagtaaaa aatgttgcta aggcagttaa agaagcagcg ataaaaacta aagtaaatag
    11821 aatataaatt tttataactt attaaaaact agaaaagtat aaaagactag tgaaattatg
    11881 tacagtggga tgattttatt agtcttttta ttttaaatat gacacaataa tgtcatatta
    11941 agcttgatat aattttaata tggttaggag ccaaagaaaa atataaggag tgatttaaaa
    12001 tggaagaaac taaattttgc caatgctgtg caatgccaat gggacaaact gatgaattat
    12061 atgggacaaa taaagatgga agtaaaagta ctgattattg tagttattgt tttaaagatg
    12121 gtgagtttac agctgatata agtatggaag agatgataga agtttgtatc cctcatatgc
    12181 ttcaaagtaa taaggatatg actgaagatg aagcaagaac aatgatgaac aatttctttc
    12241 caacattaaa acgctggaaa taattttatg caaaattagt tatatgcaaa acatgaaata
    12301 tggtgttgag aataaggggt ggtgtgcttt atagcatacc acctgttttg ttacttgcta
    12361 gttcagttat tcttgtatgc ttaaatatgt tctcatctct ctttctattt taaattgtaa
    12421 aaatctaaaa tatgttattt atattctact tctttatcca tgtctttttc tacctcaagt
    12481 caaatacaac tacaaaatca tcattttcaa ttcactgttt aatagaatta acagataaag
    12541 agttataaaa ttttctattt tttatataaa ataatacagg tcaagctgac ttctagctac
    12601 ttgggatagt gtatatattt atgatagtta taggagtaat accaatcatt agactgttga
    12661 tttgtgtagt cttttgtaaa aacatttgtt ttaatatatt gagtttttga aaaagtaaaa
    12721 ttataattat aataaattaa gtgtaataat gtattagaag taaaaataat taaaatagag
    12781 gtgattaaat gagcagtata ttgttagttg aagatgattt aagcttgata tatggattag
    12841 agttctctat acaaaaaagt ggatttagta tagacatagc aaggacagta aaagaagcta
    12901 tgcaaatgta tgaggaaaaa aattatgact tattgctttt agatgtatcc ctacctgatg
    12961 gtaatggatt tcagatttgc gaaaaagtac gagaggtttc taatataccc attatatttt
    13021 tgactgcttc agatgaagag gtaaatgtag tgatgggtct tgatatgggt ggcgacgatt
    13081 atataacaaa accttttaaa ttaaatgaat taatttcaag aataaaagca ttacttagac
    13141 gttataatgt tgcaagtaat gtgaatgaat taagttcaaa taatattact attaaattac
    13201 ttgaaaatcg tgtttttaaa ggtgaatttg aggtggaact tactacagca gaatataaat
    13261 tattgtgctt atttatgaaa aataaaaata ttgtacttac tagaaaaaac atattggaca
    13321 agttatggga tggaaatggt agctttatag atgacaatac attatcagta tatgtaagac
    13381 gattgagaaa taaaattgaa gataatcctg aaaatcctaa gtttctattg accgttagaa
    13441 gaatgggtta taagtggaat gtaattaagt gagggtatta atatgaagat attttctaat
    13501 aaagatataa aaatattttt tttaattctt atatctattt tagtaatgtt tattatagta
    13561 gggcaaatag tggttataag tataactaac aattataaaa aaacgttgat agaacatgat
    13621 tataatatag ctggatactt atatagtata ggtgttgata aatctaaaat accatcagca
    13681 tttgttgcac atgaagataa atatatttta aaaggaaaag aaatactaga tacagctgga
    13741 tatggatttg atatagatgt tagctttaca ccaagtgtaa aattgtttca aagtaaatat
    13801 ataaaaacta tgtctatcta tttattagta ttttttacta tgattatttt catactatat
    13861 atgtattttt ttaggcaaca aagtaagata gaagaggcta gttataaaat tgatttattt
    13921 atgaatggaa atataaatga aagactaaat acatatgaag aaggtagctt atcaaaatta
    13981 tttagttcaa tagatgttat gtcgacatct ttaaatacac atatattaaa agaaaaacag
    14041 agcagagaat ttcttaaaaa tacaatttcg gatatatcgc atcaattaaa aactccactt
    14101 acagctttaa taatgtacaa tcagatacta caagaagaaa gtcataatag tgatgtcgtt
    14161 gaagcttttg tacaaaaaag tcaaagtgaa ctagaacgca ttgaaacatt gattaagaat
    14221 ttactaaaga ttacaaagat agattcaaaa acaatagttc tcaataaaaa taatgttaat
    14281 cttaaaaatt taattgtcaa aattttgtat agctttgaaa ctagggcaga aaaagaagga
    14341 aaggttattt ctataaatgg ttcagaaaat acattgttgt tctgcgatta tgaatggatg
    14401 tcagaggctt taagtaatct tataaagaat gcattagata atacaaaaga gaatgataaa
    14461 ataacgatag agtggtttga gacgactata accaccattg tttcagttaa agacactgga
    14521 aatggaatcc atgttgagga tattcatcat atatttaaaa ggttttatag aagtaagttt
    14581 tcaaaagata atcaaggttt aggactggga ttaccactag ttaaatctat tgtggaacaa
    14641 cataatggta ctatttctgt agaaagtaaa ctatcacaag gaagtacatt caccataagt
    14701 tttttaaagc ttactaatat gtaagataaa atacatcttg cagtaaggta tatagattat
    14761 tattatatta atcaattaga aaggtgagag agaaaatgga tattcttgtg gttaataatt
    14821 tatgtaaaac atatggaaat ggatatacaa aagtagaggc attgaaagat gtatccttta
    14881 cagtttcaaa aggagagttt gtgactgtag ttggagaatc tggttcagga aagagtacac
    14941 tattaaatct cttaggaggt cttgatactc caacatcagg gagaatattt attgatggta
    15001 aagaaatatg ttcaatgaaa gaggaaaaac agactatttt tagacgtcgt aacatagggt
    15061 ttatatttca ggcatataac cttataccag aactaactgt ggagcaaaat ataatattcc
    15121 cattgttatt agattataaa aaaccagata ctatttactt agatgaagta ttgactattt
    15181 taggtcttaa agataggaaa aatcatcttc caaatcaatt atctggtggt caacaacaga
    15241 gagttgcaat tggaagggct ttgattacaa agccaagtat tatactggca gatgagccaa
    15301 cgggaaatct tgacagtaaa aatagtaatg atgtaatttc acttttaaaa gtgactgcag
    15361 aaaagtataa tcaaaccatt attatgataa ctcataatac gaatatagct tctacagcag
    15421 atagaatact acaggtttct gatggtgtgc ttacagacct gggggtgaat agagaatgag
    15481 aagctactta gaattagtac cacaatatgc aaaagtacat aagaaaaaaa atagaactac
    15541 aattttatgt attattattg cagtatgctt agttactgct atctttggac ttgcagatat
    15601 ggctatacaa tgtcaaaaga tacaatttat taaagatgat ggaaattggc acatagtaat
    15661 tagaaatatt gatgaaaagt ttatatcaga aataaatagt agagtagata tagctcacca
    15721 tggtaggggg caagtcgttg acagtggaga gtttaaatca aaggagattt tgatacaagg
    15781 tttagatgaa gaagtagcaa aacaatttaa cttagaaata ataaggggtc gttatcctat
    15841 tggggagaat gagactttaa tagatgagga tgcagttaaa agctttaatc taaaaatagg
    15901 agattttatc aaaataaaat tagcagatgg tagaaataaa aattataaga tagtaggtga
    15961 atattcatca atatcaagct taagagctac agataaacat ggattaatac tttcaatatt
    16021 gggtacacaa gctattaaaa aatcagaaac gtacgattat tattatatta catttaaaga
    16081 aaaggttaat attagaaaag ccacttctga tattaaaaat accttcaatt taagtgataa
    16141 acaaatcggt aaaaatgaaa gattactttc tattataggt caaggcgact ctgacttttc
    16201 taaaccactg tatttaacag caggattact ttttgtactt gttttagtag caagtattat
    16261 catgatatat aatagtttta acatgagtgt aattgagaga gttaaatttt ttggtctgtt
    16321 acgttgtcta gggtcatcaa aacatcaagt taaaaaattt gttgttttag aaagtttaat
    16381 tttaagtata aaagctattc caataggctt agtattagga agtcttgtaa ctattatatc
    16441 ttctatattt cttaagtatg ttaatgagtt attcagctca atgccaattt taaaagttag
    16501 ttttattggt attgcatttg gaataattgt agggttttta actgtaatat tatcagctat
    16561 tattccagca aaaaaagctt cccgtgtatc tccacttagc gctgtacgtg ggaatctaat
    16621 gtccaaagaa attttaaata acgggaagac aagtaaagtt gcaaatacag gaagttttaa
    16681 aattgagact tctatgggat taaatcatgc tttttcaaga aagaaatctt ttgtacttat
    16741 gacttcttca tttgccatta gtattatact atttttaggt tttagtgtgc ttatagacct
    16801 tatgtatcaa ggacttaagc ctatgaagcc agaagctcct catatatcta ttgttgaccc
    16861 taattttaag tctgttttaa ctaaggaaga tattaatgaa ataagcaata tcaaaggagt
    16921 aaagaaagta tacagtagaa tgatgacgga tatagaagct acatataatg ggaagaagac
    16981 aaaatcacaa ttaatttctt atgaaaagta tcaatttaat tgggcaaaaa aatatctatt
    17041 aaaaggagaa gttaatgaaa ataaactaca gtcagaaaac cttgcattag tagaagaggg
    17101 gcatggattt gatataggag ataagatata tattaaaaat agaaatgctg aaaaagagtt
    17161 agaggtagga ggtatacttt cctcaattcc atttgaggct aaagataaat atattggaaa
    17221 tattataaca tctgaaaata cttttacgaa tataacaggt ataaagggtt atataataat
    17281 ggatattcag gttgataaag atactcctgg agatttaatt gaaaatataa gacgtgtagt
    17341 agatgataac ttagaggtca gagataaaag acaaagtaac ttagaggtta aaaattcgtt
    17401 ttatactatg gcaacatttg tatatggatt tgtgtttatt attgcgatta taagcatgtt
    17461 taatatcata aatagcatga atataagtgt tacaagtaga aagaattact atggtgttat
    17521 gcgtgcagta ggcatatcca acagacaatt aagaagaatg gtaatagtag aggcaggttc
    17581 atatgcaatg agtggttgtt tattaggtag tatactagga ttaatgttac ataggtatat
    17641 gtttatatcg ttagtaagtt caatgtttca tttagagtgg caagtacctt ttaatttact
    17701 acttatcact gtactaacta tgataataat aactttatta tcagtaagaa aaccagtcaa
    17761 aaagatttgt gaattagata taatagactc aataaatgca caataaatat agctgttgat
    17821 ttaaattaaa aaaacgaagt aaaaatgaaa tagaaaatat ttttcatttt tacttcgttt
    17881 tttatattat aaaatttttg ttatgttaca aaatttctat aaagatgatg tgaatctaaa
    17941 cattatatga caacgtgagc atttaagtta aaatatttta aatatactat tttagataag
    18001 ttccttcttc atcttgataa attaatcctt ttttcataag cactccaaga gctcgtttaa
    18061 aatttttctt acttgaatta aataatatag aaatatcttt agggtcagat ttatcattaa
    18121 atctcatata tccatcagaa ccctctaaat aacttaaaat cttattttct aaagtatcaa
    18181 gttcatcctt tctatttcct ctaggagaaa gaccaagttt tccatcttca tatattttta
    18241 tgacatgtaa attttcaaga acatcaccag cttttagttc agtaaagtac tcattatgaa
    18301 gtattacacc agcatattta ttgtctacac atatcattgc agaattatta gtttgaaatc
    18361 catatacaac tccagttaca atatctccta cattgtaagt atggtctgtt aataaatatg
    18421 aatctatatc tgtagtagca gcaattcttt tgcttttgtc taaatatata taaaataaat
    18481 atttttcgtc tctaaatagt ggataagtct tagctttaaa aggaactagt atgtctttag
    18541 gaagtcccat atctataaaa gtaccaatgt ccgtattatc aactactttt aaatgtgcta
    18601 catccccaac cttagctaaa ggcggtatta aagttgcagc agttctgtca tcagaatctt
    18661 taaatatgaa agcatttact tcatcaccta tgtttatttc attttttccg attaatctat
    18721 tatgaagaag tatatcgtct tttgtgttat tagtttgtcc atccaaaaaa tatccaaatt
    18781 cagtttttct ttttactgta agtttattaa aatcacctat gtttatcaaa ttaaaatcat
    18841 cctctcattt tatatttata atttagccat aatatactta tgtagtagtt tgtcacagta
    18901 tctatattag tattagtgaa atttacaata aataatatag ttatttatgt atttttcaat
    18961 tctctagctt cttatatagt ataaactcca atttcttttt tgtctttagg ttttactaaa
    19021 atttttacaa aataagttaa aaagtattga aataaaattt cttttaaagt ataattggta
    19081 tataccaaaa ttggtatata ccatatcgaa aagtggtgac tatatgaaaa tagtaaataa
    19141 atctagtaat ataccattgc atactcaatt aagttcaata ataagagaga tgatagaaac
    19201 tggtgagctt aaagagggtg atgcaataat gccagaaaga gaactttgca acatacaaaa
    19261 tgtaagtcgt atgacagtaa ataaaaccat tgttggatta gtcacagaag gacttcttta
    19321 tagggtacaa ggtaaaggga cattcgttgc aaagcaaaaa aagaagtatc agttttcaaa
    19381 tgtaaaaggt tttactgatg ttatgaaaga aaagggagta aacataaaaa cggatatatt
    19441 atcctttgag atggaactac cagatgattt agttaaaaga aagcttggaa taagtgataa
    19501 tactacaaat atatataaga ttgtcagact tagatatact gatggagagc catttggatt
    19561 agaaatagta tatctatcag aagaaatgtg taaaggtcta acaaaaggga tattggataa
    19621 ttcttcatta tatagggttt taaatgaaaa atatggctat aagatacaaa aagctgaaca
    19681 agtaatggag ccagtaatac tttcagatga agaaagtaag cttcttgaaa cagatgaagg
    19741 tgctttagct cttaaacttc atagaaattc ctataataga gaaggtagcc ctatagaata
    19801 taccatatca atatttagaa cagataaata tcaatatgaa atagtattga gtgagtaggt
    19861 gaataatatg aaatgtttaa ttaatggaaa gataatatta aaaaatcaaa tcttggaaaa
    19921 taaagtatta gtatttgatg aaaaaataat tgatatagct gatagtgtac caaaagattg
    19981 tgaagttata gatgctgatg gaaaatatat atctccaggg cttattgata ttcatataca
    20041 tggaaatatg ggaaaagata caatggattc gactgatgaa tctattgaaa caatttcaaa
    20101 atcaattatg agacatggag taacatcttt tcttccaact acaatgacta tggataaaga
    20161 acatgtttat gatgcacttg aagttattaa aaaagcccaa aatagaaagc ttgaaggggc
    20221 acaagtatta ggagcacatt tagaaggacc ttttataaat gagaattata agggagcaca
    20281 aaatgaaaaa tttataataa attcaaaata cgaatttata aaagaatata aagatgttat
    20341 aaaagttata acttatgctc cagaaaaaga tattgatttt gattttacta gagaaataaa
    20401 aagatgtaca gatatagttt tatctattgg tcatagtaat gcaaattatg accaagctaa
    20461 agaagctata aatttaggag taactaatat aactcacatg ttcaatgcaa tgactggttt
    20521 aaaccataga gacccaggcg ttgttggtgc agcacttaca actaatgtgt acagtgagct
    20581 aatagcagat acaatccata taaataaaga tttatttcaa tttatactaa ataataaagg
    20641 gaaagaaaga ctgattttaa taacagattc tatagaagct ggtggtcttg aagatggcaa
    20701 ttattctctt ggaggtcaga aagtcatagt taagggtaat gaagctagac ttgaaaatgg
    20761 agcattagct ggaagtgtgt tgtctttaaa taaaatggta tttaacttct tagataatac
    20821 aaaccttaaa gtgaatgaag caattaattt agcttcttta aatccagcaa catctttggg
    20881 aataaatgac aaaaaaggaa gcttagaaat aggtaaggat gcagatatag ctgtatttga
    20941 tgagaattta gattgtaaaa tgacactttg cttgggggac gtagtatata aaaatatata
    21001 gtatacaaaa caaactttaa agacagtaaa aacaatataa aaaataataa attaaactta
    21061 cattagttaa agtagtagtt aaaatttagg aggaataaga tgagagtatt agtgtgtaaa
    21121 gattatgatg gaatgagtaa aaaagccgct gaaatgatag cagctcaaat agttttaaaa
    21181 ccaaattcaa tactgggact agctacagga agtacaccag taggtatgta tagagattta
    21241 gtaaaaaaat acaatgataa tatagtagat ttttcagatg tgatgagctt taatttagat
    21301 gaatattata agttaccaat atctaatgac caaagctatg attattttat gaaagagaat
    21361 ctatttaatc atgtaaacat aaagctagaa aatactcatc taccaaatgg aatggctgat
    21421 gatattgaaa aagagtgtct gaactatgaa gcttctatag atgcagctgg tggtatagat
    21481 gtacaagtac ttggaatagg tcgtaatgct catataggtt ttaatgaacc tgatactaaa
    21541 tttgcaaaaa gaactcatgt agttgaactt acagagtcta caatagaagc taatgcaaga
    21601 ttctttaaat caagagaaga tgttcctaaa aaagctgtaa gtatgggaat aggttcaata
    21661 ttgaagtcta agaaaatatt gcttttagct tcaggagaag aaaaagctga tgcagtatat
    21721 aatactgttt acggagatat aactccagaa gttcctggtt ctatacttca acttcataaa
    21781 gacactatag ttatagtaga tgaagcagca gcaagtaaac tgaatccaaa agattataaa
    21841 ttagtataag aatagaatgt agtttaagtg aaaaatagct atcattgaat aatcaaattt
    21901 atttaatgat agctattttt atttaaattt tttacttctt tttatttaaa ttagttatta
    21961 ttgtttaaat tacttgctta ttacttttta cgaaaactcg ttatcaagaa gtatccgaat
    22021 aagatgttaa aaattattat gtttagtggg attctatcaa gcataataaa attacctata
    22081 attgggatat taccaattgg aaacataaag aagaatacag gcatcagtga tagtaaaaaa
    22141 gatggaaaac aaagaagaag taatcttgga atatcaatat accaactacc atcctttttg
    22201 tattctctaa tcaataattc agttcctagt aacaaaccaa taccaccaga ggaaattaaa
    22261 ttaataaaaa tgtatctttt aaagtcttgc atataaccag tgtgaaattg agtatagaaa
    22321 tcataattta atttaacggc taaaaaatat ataagcaata gaatgattaa aaataaaaga
    22381 tatctaagag cttttttcat tttaaattct cccttcagaa atactttatt cagttttatt
    22441 ttgttggaat ctgattttat catttagttt aagaaatcca taatattagt gaagaatgaa
    22501 ataagtatta tatatgaata aatattttta gaattaaggt gaactgtagt gcttaatata
    22561 actataatat taattgtttg aatattcaat aaaaagttta aaaaatatca attttgatat
    22621 aaactgaata aatacagttg aatattaaat tattatttta tcaaaaatat gcaaagtact
    22681 acaccttcaa atatcaccta aagtaattaa aaggcaatta aagtgaagta gttcaagtat
    22741 ttctaaaatg agtttaataa attctataat aatttatttt ttgccttaaa attgccttaa
    22801 aatccaaaca attttaaaac taatgtacta taaaaagctg gtataattta aatatagtaa
    22861 aaaataagat ttatcttatc tattgggatt taattattta atcaatatat ttataccatt
    22921 taatttaaat aaaaagtcta ttaaggagtg agcttatgag taagttaatt tatatagcag
    22981 atgatgaaga taatataaga aatttggtaa agacattctt aaaaaatgaa ggtcatgatg
    23041 ttatggattt taaaacgggt gatgaacttt tagaacaatt taatataaag gagtgtgact
    23101 tagtaatact agatataatg atgcctggct caagtggttt tgaagtatgt acaaaacttc
    23161 gtgagaaaag tacagtccca ataatcatgc ttactgcaag agatacagat atagattata
    23221 ttacagggat aaccttaggt agtgatgatt attttacaaa accatttagc ccaatgtctt
    23281 tggttatgag agtaaaatct atatttagaa ggatagagtt tgaaaagaaa caaaattatg
    23341 ataaatatag taactcgata gatatggaat taaaatttgg tgatgtaata ataaataaaa
    23401 agaataaaat cgttacaagt aaaaatgtaa atatagacct tacaccaaat gaatataatc
    23461 tattgactta tttgtttgaa aatatagata gagcagtttc cagagatgaa ttattaaaca
    23521 aaatatgggg atacgatata gaagttgaga caagggctgc tgatgataca gtgaagagat
    23581 tacgtaaaaa aattttagat acaaatattt taatagaaac tgtatggggg tttggattta
    23641 ggttgaagga gaaaagttaa gatgaataat ggaaatgaca agaatagaat tggaaagatt
    23701 aaatccaaat ttaaattaaa gttctcaaag aaaaatataa gcataaaaac taggattttg
    23761 ttgagcatac tttgtttgat aattatagta tttatgataa tattagtatc ttttaatgta
    23821 tttcttgata catatataaa aacaactgcg aatgaagaat tgacgaagtc tacagaaata
    23881 gtagaacata tggatagtaa ttttagacct attaagccac cacaagatga tggaaagctt
    23941 cctcccaaag tattatctaa ttttatgaga aatgtacaag ataaagttag gatggcagaa
    24001 acacaatcag atgctgatgc tatggtagtt gattctaagt ataacctgat atttccaaca
    24061 agagaggatg atttcttaaa aaatgttgat gagatggagc tgattaggga gtgtattcaa
    24121 aatgaaaaat tagatttaaa ttcagatgaa aatacacgga tatctaccag aaataaggat
    24181 tattatattt caactgtaaa aattactagt atatatggtg aacaagacaa atatcttata
    24241 ctttttatag acatatcaaa aactctaaat ctagcacaaa agataaatat tgtattaata
    24301 tctgtaatgt gttttgctgg aatattagca atatttacag ctgtgatttt atctgagaaa
    24361 attgctaaac cgataaaaga gttgtgtgag tttgcaaaaa aaatgggtca aggagatttc
    24421 aaaagaacct actttgactt ttcagataaa gaattagtag agctctcaac tgttatgaat
    24481 aagagtgctg agtatctgga taagtatgat aatgaacaaa aaatattttt tcaaaatgca
    24541 tctcatgaat taaggacacc actcatgtct attaaaggtt atgcagaagc tataaaatat
    24601 aatgttatag atagtaaaca tgcaagtgat ataattcttg aagaaagtga taggttaagt
    24661 gatatggttg aagatttatt atacatttct aaaatagata atataactaa agattttgaa
    24721 cttgtagaat gtgatttaag agaagtattt tcaaattgtg gagctaggca aaatgtaaga
    24781 gctataaata aggggataaa atttatttat gaatttgatg aagaaatggt tttatttgaa
    24841 tgtgatgaaa aaaacatttc caaagcattt atgaatctta tagaaaatgc tttgagatac
    24901 gctaagagtg aaataaaaat agtgtgtaag tataatcaaa aaaatatagt tgtgataata
    24961 gaagatgatg gaattggaat caagaaagaa gatttacctc atgtttttga aagattctat
    25021 aagggagtag ggggaaatca tggaattgga ttatcaatag ttaaatctat tgtaaataag
    25081 catggtgggc gtatatacgt agaaaatggc aaaaaaggag ctaagtttac tatattattt
    25141 aaattgtaaa aaaattagag aaaatataaa gatgtttgaa gttgtttttt atatatatct
    25201 gttgtatact gttgaaaaaa gaacaatatt aagctgacat atattacaat aattgacatt
    25261 taaagtaaat gcttaaatat agatacgaaa ggaaaagtaa aatctgaagt gtatttaggt
    25321 ttaagtaagc ttcttatttt ataaaaaata tgtatgaaag aacagctgat ttttaatatt
    25381 acaaatttag taacggttgt atttgaagct tttgtcatcc atatgttttt atcagacttt
    25441 ttaggtcaga aagaagggta tataaaagtt gtaagatatg ctaaactggg ttttataata
    25501 tgtttaggat tctgtaattt aataacttta aaccctaaaa tcacaatgcc tttaattttt
    25561 atccttattt tttcaacatc atttttatat aaaggaaatt taaagacacg attgtttact
    25621 actgttttat taagtatatt ctttatactg tcagaaattg tagttacaag tatttttgtt
    25681 ttatttgtaa aagaaggctt tgaaataatg cttgaaaata atagtataag agtattggcg
    25741 acaattttat ctaaaattgt ctttttgttg acttgtaaaa ttatttgtct atttaaaaaa
    25801 gatgtacatt tagatatgcc cataaagtat tggctccctt tatttttaat acctatattt
    25861 agtttatttc tgtctgtttc tatctttgat gtaagtaaat tttttagtct tgaaagttta
    25921 aaatttctaa gtttaatatc tagtgttggc attttatata ttaattttat tgtattt
//
LOCUS       DAMJWP010000034        25920 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.035, whole genome shotgun sequence.
ACCESSION   DAMJWP010000034 DAMJWP010000000
VERSION     DAMJWP010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25920
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.035"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(98..1105)
                     /locus_tag="PC700_001207"
     CDS             complement(98..1105)
                     /locus_tag="PC700_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HDF2580523.1"
                     /translation="MQLKNFHFKNNTLSKKTNLAKSKFTIVLTFLFLLYFFSNNLIYA
                     QNLQKNEPEYLIPIGNVLQIDAELKNIIVRNPGEASPFRLGDAIVKVNNSEVDGYSDF
                     ANTLNALPEEKQVSILLNRGGQLITMKTTKHILEKISFNNLLSGFATLTYINPDTSEF
                     GAVGHPISVGNARKIPIKTGSISTTKHLNIEKSYKGSVGCINAKRENIIGSFTENTDF
                     GIRGQINNFDISNLKKYKVASLDEVKLGKAQIILQDNFNKCKKYNIEIIDIENQKHPE
                     SKSFKIKITDKKLLAQTGGIVQGMSGTPIVQDNKIIGAVSHAIENDPAVGYGVFIKWM
                     L"
     gene            1333..2217
                     /locus_tag="PC700_001208"
     CDS             1333..2217
                     /locus_tag="PC700_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2580524.1"
                     /translation="MKKYRLLVTCIIAMGIIIIGTSNPVSNFYKTTNLISSGNITNEK
                     ADKDENSNKNNKDKNEDKSNSKSNKDKSSEDKSSDKKNKIQKNKKLLICIDPGHQGKG
                     DSNLEPVAPGSSSKKARVSSGTEGIATKKPEYVLNLEASLVLKSILESKGYNVIMTRE
                     THDVNISNSERAILANDKKADMVVRIHADSLNNSSKTGASILIPEKDGKYTAPIYEES
                     NKCAEFIKQNMEQSGIQINGIVQRGDLTGFNWSKVPAVLVEMGFMSNYNEDQMMSNPD
                     YQRKMMQCIADGLDAYFK"
     gene            2419..3969
                     /locus_tag="PC700_001209"
     CDS             2419..3969
                     /locus_tag="PC700_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF2580525.1"
                     /translation="MLVVENVSHGFGARTILENVSFRLRKGEHIALVGANGEGKSSFL
                     NIITKKLMPDAGNIKWSSRATVGYLDQHTVLSKEKTIREVLREAFKHMFDLEQEMIAM
                     YDKMGEASDDEMSKLLEETAEIQTILENSGFYMIDAKIQEVANGLGLGEIGLDKDVTD
                     LSGGQRTKVLLTKLLLENPTILILDEPTNYLDEEHITWLTKYLQEYENSFVLVSHDIE
                     FINNTCNVIYHMENGELNRYKGNYDEFVRLNDIKKRQEEQAYDKQVEERKRLEDFVAR
                     NKARVATRGMANSRQKQLDKMEILERPKEKIKPTFAFKDARAASKIIFETENLVLGYD
                     EALTKPLNFHLERGKKIALKGMNGIGKSTLLKTLLGIIKPFEGNVKLGDYLEVGYFEQ
                     ESSRENSNTPMDEVWSEFPGLTNFEVRQALAKCGLTNEHITSQMRVLSGGEAAKVRLC
                     KVMLKNINFLVLDEPTNHLDVEAKDELKKAIKEFKGTVLLVCHEPEFYSEIVDDVWNI
                     EDFTTKIV"
     gene            3998..4633
                     /locus_tag="PC700_001210"
     CDS             3998..4633
                     /locus_tag="PC700_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HDF2580526.1"
                     /translation="MKKFLNVLFSVCISIVIIVGVINFTVGFKQLYYFDIDYLNISEL
                     SGLSEDDIKLNYDYLIDYNLNKNVSEFKLPTLKSSPQGKIHFEEVRNIFQNINKLAKL
                     LLVVFLVGIILSVKNKNIKILKTTSITLIIMPLLLTVPILLNFEKSFIIFHKLLFRND
                     YWIFNPNLDPVINILPEEFFFHSGMMILILILLASILLFVMYRLYKSFKIK"
     gene            complement(4744..5004)
                     /locus_tag="PC700_001211"
     CDS             complement(4744..5004)
                     /locus_tag="PC700_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich small domain-containing protein"
                     /protein_id="HDF2580527.1"
                     /translation="MGENYKFFNHKDCEFFPCHKTNKPEEFNCLFCYCPLYALGENCG
                     GNFKYTDKGIKDCSSCMLPHKKDNYNYIMGKFQDLVKITSKK"
     gene            5221..6504
                     /gene="hflX"
                     /locus_tag="PC700_001212"
     CDS             5221..6504
                     /gene="hflX"
                     /locus_tag="PC700_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDF2580528.1"
                     /translation="MKEIQEKALLVGLNLTTIVKKNDDIDTSESMEELKELTKAAGAE
                     VVGSLIQNKHSVDAAYYIGKGKVEEIRAYSDSLDATLVIFNDELSGAQIRNIENVVGR
                     KVIDRTTLILDIFAQRALSKEGKLQVELAQLKYRLPRLYGMGGEMSRTGAGIGTRGPG
                     EQKLEIDKRHILNKAADIRRELKEVKKNRETQRVKRLKSNIPIVALVGYTNAGKSTLL
                     NELIKTHKDYEQEKEVFVKDMLFATLDVTLRKALLPNKKEFLVVDTVGFVSKLPHDLV
                     EAFKATLEEVQYADLILHVIDATNTSYELQKSTTEGVLKELGVNDKKHILVYNKVDKL
                     ELDIYPKSQEDIVYISAKQGINMDKLLNMIEIALMENTYSVSLMLPYERGDIFSRIKD
                     KYNVENFEYGENGITLDVNFDEEDFNIYREYILEK"
     gene            6512..7189
                     /locus_tag="PC700_001213"
     CDS             6512..7189
                     /locus_tag="PC700_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HDF2580529.1"
                     /translation="MDINWENIDNLEDYFITYLLYKESKTVSQISKIRNISSTEAGEQ
                     LIQAKLKIKEMQKDDFEASKDILDKFLELDKIKRLDFMDSLDDEKMVYFKRKVFKRIL
                     VEKNAEDLIVLIWATGELKDDRFLKLLHQLTNHRHSDIRRITYSAIRKIESPSSREVL
                     QKGLYDKNAQTRQYCAKALSKLGDENSLKILQQLKDKNKNFEKEYVLRAYDEAIKSLE
                     NAKNLKK"
     gene            7275..7718
                     /locus_tag="PC700_001214"
     CDS             7275..7718
                     /locus_tag="PC700_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF2580530.1"
                     /translation="MIVRRCAGGVVFYANKVLIVKNDRGEWTLPKGKILGGGLPYESA
                     VQRVKVETGIDAKMIDVAGDTMYEFFSRSRQQEVCNAIMWYVMEACNTNCVLAPEFQE
                     GGFYKVKDALDMLSHHKEQALVEVSYKKFKELKKLVSEGDDVPAN"
     gene            8024..8668
                     /locus_tag="PC700_001215"
     CDS             8024..8668
                     /locus_tag="PC700_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HDF2580531.1"
                     /translation="MSNYRTLHEFGTDEITIEKSVFIGYAKPIKSEEEALEFINEIKK
                     KHKDATHNVWAYTVGKNMNIQRYSDDGEPQGTAGIPTLEVIKKEDLRDVVVVVTRYFG
                     GTKLGAGGLVRAYTKGAKLGLEAGKIIYKVMYQEVKVKIDYTQLGKVQNELMNLGYFI
                     KDTVYEDNVEIVVYSRLEDVEKLSEKMIDITNGTGKIILGEEFYLSEQNGEILI"
     gene            complement(8754..9131)
                     /locus_tag="PC700_001216"
     CDS             complement(8754..9131)
                     /locus_tag="PC700_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDF2580532.1"
                     /translation="MLFALIGFFAGIIGGMGMGGGTILIPALVLLAGIDTKIAQSVNL
                     LSSIPMTLIALAIHIKNKNVIFKLVIPIAVFGVLGAVFGSLLANYLSSDILKKIFGIF
                     LLLVGLFEVKKGFCAKSDKKPLN"
     gene            complement(9131..9517)
                     /locus_tag="PC700_001217"
     CDS             complement(9131..9517)
                     /locus_tag="PC700_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDF2580533.1"
                     /translation="MKKTDDLKKKYFDFNLKNTIIGVFTGFINGVFGSGGGTLLVPIL
                     NDIVKVEEHKSHATALSIIIFLTTASSVLYVSKGTYDVNLTIKVAVGSILGGIIGAKL
                     LNKVTGKFLRISFGIIMIIAALRMVF"
     gene            9696..10451
                     /gene="nadE"
                     /locus_tag="PC700_001218"
     CDS             9696..10451
                     /gene="nadE"
                     /locus_tag="PC700_001218"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HDF2580534.1"
                     /translation="MSNIKIQIDKTVEWLINKVNEANAKGLIVGVSGGIDSAVVANLI
                     KKAFPENSMGVIMSIKSNPQDREDALKVIEGCDIEYLDLDLIEPQSAILDMVVGNLKD
                     KHLYRAEYLKMTDANLRARVRMSTIYTIANNLGYLVVGTDNAAEIHTGYFTKFGDGGV
                     DILPIANLTKGEVYEWAKELGVHEDLINKAPSAGLWEGQTDEDEMGTTYNMIDAVLEG
                     RLDEVPKRDQEIIERLHRLSEHKRKTPAQPPKF"
     gene            10534..11253
                     /locus_tag="PC700_001219"
     CDS             10534..11253
                     /locus_tag="PC700_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF2580535.1"
                     /translation="MGRIGNIINRKGKQDAQRAKIFTKHARAIAVAAKEGGADPEYNA
                     ALKTAIEKAKADNMPNDNIDRAIAKGAGAGAGEDYETIVYEGYGPGGVAVIVETLTDN
                     KNRTAGNVRYYFDKNGGNLGTSGCVSFMFDKKGQILVGLGDGVSEEELMDVALEAGAE
                     DFITEEDGYEIITTPEDFSSVRDELKAKGYEFISADVKMIPQTTTVLTEESHLKMMNK
                     LVDMLEEDDDVQDIYHNWEVE"
     gene            complement(11421..12425)
                     /locus_tag="PC700_001220"
     CDS             complement(11421..12425)
                     /locus_tag="PC700_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDF2580536.1"
                     /translation="MATIKDIAKDCGLSSATVSYVLSGKGNERRIPLHTQELILNSAD
                     KLGYKRSTPIKHNNIKIAFYWQDEGFELASTNILQAINHIISLEHLNINIIIRPYKVG
                     HLCDDEYFFANNYYDGIVLTGINADDIEYLVKNKPKIPVVVVNREIPGFSSVSIDNEE
                     TGRIAALHAIQNGKNDIAVVFNPTSFTCMTLRGKSFLKTCKEHGIHLSDSIYYCSNKI
                     DSGYDIAQKMLAKNSLKKVIFCNHDVVALGMMNAFHESGIVIGEDIQIFAASNGPSRL
                     MARSIPPLTVIDLKMEETAERSLRMIIDTVTGRINGIQQQVIYPSIIYRQSSPAQNLL
                     "
     gene            12691..13587
                     /locus_tag="PC700_001221"
     CDS             12691..13587
                     /locus_tag="PC700_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HDF2580537.1"
                     /translation="MSKVNIFEVGPRDGFQNLKEFLETDKKLKIIDGLIASGIKQLEI
                     SSFVSPKAIPQMKDAKEVATYCVEKYPDVKLYALVPNLYGAKVAWECGIRDISYVISL
                     SETHNKKNINRTHEESIDELKKIMNTYPDMNICIGMATVFGCPFEGLPKIEDAIRFAK
                     RLWENGIREINLADTIGIADPKQVRETISALVEALPECRWQVHIHDTRNMGMVNTLVA
                     IESGITTVQSTLGGLGGCPFAPGASGNTATEDLVYMLERMGYDTGVDFVKLLELSKYQ
                     KSFVNGVYSGHHVNIKSDSCNC"
     gene            13725..14987
                     /locus_tag="PC700_001222"
     CDS             13725..14987
                     /locus_tag="PC700_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580538.1"
                     /translation="MDIIALLLFLGVIIIAFVRKNNAGILALAIGVIAVRIFGMDDKE
                     LYGAISNSMFTTLVGITLLFAIVNSTGALKLVAEKIVAASGKRVWLIPIAVFIAGFVV
                     AGVGPGAIPALAIIPAMAVPVAITVGYNPVMLALIGECGLMAGRMTPITPEGQIITTV
                     AEGVGIENVMPTILACQTLSTIVFSIVLFIIFKGYKLKKPINVIDSKQIEKFNIKQII
                     SLISIVVMIAFIIIFDMNVGLAAFAVSAVLIFFNIADEGECIKSIPWSTIVMVLGVGA
                     MMTIVDAAGGIDLMSNALSSLMNSKTATPIMSISAGLMSMVSSALAVVYPTMMPMCVD
                     IAKAVGGVDPLALIAAVGVGGSLAGVSPLSTGGALILAAMGSSKKDFNKEEQNKVFVQ
                     LFIMSAVGLLVIAVVSILSFNAITHLMN"
     gene            15063..16256
                     /locus_tag="PC700_001223"
     CDS             15063..16256
                     /locus_tag="PC700_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HDF2580539.1"
                     /translation="MKKGALSNIKVLDLTRVLAGPYCTMMLADFGADVIKIEMPRKGD
                     DTRGFGPFVNGESLYYANINRNKKGITLNLKSEEGKNIFLEMVKHADVVVENYRPGVM
                     EKLGLGYDELAKVNNQIIYGAVSGFGCYGPYSERPGYDIIAQAMGGLMSITGQEGGEP
                     TRVGNAMGDVLGGMNLTIGILTALNARKIIGKGQRVDVSLVDSVVSSLETGIQRYFDS
                     GQPELMGNRYASAYPYDSFKASDGSFVIGCGNDKLFEKLCKKVLFREDLLIDERFKTN
                     LKRCEHYKALKPEIEKWSVKHTIKEAVDLISEAGVPAAPIYNLDCVTTDEHIAKAREM
                     IVDISHPIIGNMKVNGNPVKLMTTKAKIDMPAPSLGESNEEIYNGWLGFDSDKIKDLK
                     ENGVI"
     gene            16885..17679
                     /locus_tag="PC700_001224"
     CDS             16885..17679
                     /locus_tag="PC700_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HDF2580540.1"
                     /translation="MESVMENLNNLKVEVKDKVCVITINRPKALNALNSDTLRELSQV
                     IDVVSENEAILGVIITGEGKVFVAGADIRQMQNYKSEEGRKYAGYAQGIFDKIEALEK
                     TVIAAVNGYALGGGCELAMSCDIRIASEKAIFGQPEVNLGVIPCFGGTQRLSRLVGTG
                     IAKELIFTGRQVNAEEAKSIGLINKVVPSDLLLEESMKMMNQIVEKAPIAIRYAKVVI
                     NKGIDMDLKNALELEKDIAGLTFATRDKQEGMNAFIEKRKPVFENK"
     gene            17773..19113
                     /locus_tag="PC700_001225"
     CDS             17773..19113
                     /locus_tag="PC700_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDF2580541.1"
                     /translation="MNTGVIISLVGIIIALSLLVVMVMKGINIFIIAIVCSSLVAITG
                     SLNVYDALKVDYMTGFVGFLQANFFIFLTGTLMGKIMEITGGAKSIAKMIVRWIGKDK
                     ALLSIPIACGILAYGGVSVFVVSFAVFPIALEIFKEADLPRRFIPAALTFGCSTFAMV
                     APGAPQIQNIVPASTLGTDIMAGAVNGFISCAVMFIVGSTILYKMVSKEKANGGHFIA
                     HESDIFDEAEATNSKRENGPNGVIALIPLIVSILIINVKVNGKAIVPIEVGVFIGALL
                     VYLLLNKYQDNTKIVGHVGDACKTTVVAICNTCAVVAFGSVVKSAVGFDFIVNAMTNI
                     PGPPIAGAAVGTTVIAGICGSASGGLGIAAPLLGPVFLAKGVSAAALTRTMAISSAAL
                     DSLPHNGYIVTVTNGLCKETHKDAYMPIFWVTVLTPFIGTIVAVLLFTMFPGLP"
     gene            19250..20482
                     /locus_tag="PC700_001226"
     CDS             19250..20482
                     /locus_tag="PC700_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HDF2580542.1"
                     /translation="MLNKPLEGVKVIDLTYFVAGPGTSKILADWGADVIKVEPSFGDP
                     GRKTGATMTMPIDDYNNPFYSTYNSNKRGLSINLKSETGIEIMDKLLSEANVFVSSYR
                     TGALKRLGLDYESLSKKHPHLIWGQINGFGDFGPAKDNAGFDTVAFWARSGAMLDIAE
                     KDTSPINPPIAFGDATTSCSLSGGICAALYQQAKTGKGQKVMASLFGQAIWNASSLLA
                     STQFSDEYPKTRKNAISPVINSYRCKDGKWIFLSILEHERYFEALCRLFGREDLIKEE
                     KFASSVESKKNATELINILDGEFAKFTQNEMVEKLTAADIAHEKIQHVNDVVTDEQAI
                     ANNYIYEHTSKNGNKTMLASTPVKFGNIEVNMTCDAPLIGEHSDEILRELGYSDSDIE
                     ALIESGIVTMNKEACEIC"
     gene            20522..21664
                     /locus_tag="PC700_001227"
     CDS             20522..21664
                     /locus_tag="PC700_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HDF2580543.1"
                     /translation="MDFRLTEAQLMLQRVAKEFAENEIAPIAAETDKTGIFPRELFSK
                     MAKIGFNGIGTPVEYSGSGGTDIEKVIVVTEIAKKCAASAAILSIHTIYAQAILKFGT
                     EEQKKKYLPMMAEGGCVGAFALTEPNAGSDAARAATTAIIDEETDEYVLNGTKCFISG
                     GGQAESLIIFALTDPSKGIKGMSAIIVDKGTPGFSIGKIEEKMGIHGSETAELIFDNC
                     RVPKSNLLGKEGKGFNIAMTCLDGARIGIGAQAVGIAEGALEESIKYSKERVQFGKPI
                     SALQGIQWYIADMATMVESAKLLVYYAADLKARGEKHTKEAAMAKYNASSTAREVTNL
                     ALQIHGGYGYMKDYPLERMYRDAKITEIYEGTSEIHKVVISRAVLG"
     gene            21675..22469
                     /locus_tag="PC700_001228"
     CDS             21675..22469
                     /locus_tag="PC700_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDF2580544.1"
                     /translation="MNIVVCLKQVPDTNEVKINKETGTLIRDGVPSIINPDDRNALEE
                     ALKMKDELGAVIKVISMGPPQAKSALKEALAMGADEAYLISDRAFGGSDTWATSTIIA
                     AAIEKVGKYDVIFCGRQAIDGDTAQVGPEVAEFLGIPQVTYAKEVKVQDDKLLVTRYT
                     ETGDYLIEAKMPVLLTAIKELNNPRYPSVKGILEAYNNGDAKITVLTLADLDVDTTQI
                     GLKGSPTNVYKSFAPVKDKHNEIIEGINKKEKAEKLIEILFDLKLV"
     gene            22480..23502
                     /locus_tag="PC700_001229"
     CDS             22480..23502
                     /locus_tag="PC700_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDF2580545.1"
                     /translation="MMRAKVNQGINLNDYNGVWVIGEQREGKINPVTIELIGEGRKLA
                     DQLGKELEVVIAGYEVEKEVKELLHYSVDKVYYINDPLLKDFTTDGYAISIANLIERK
                     KPEVVLVGATSIGRDIAPRIAGKVGTGLTADCTKLEIDSTDNKLLQTRPAFGGNLMAT
                     IVCPKNRPQMSTVRPGVMAKAVRNESETGILEVVTPELTEKMIRTRLVEVLPQEKKSV
                     NLTDARIIVSGGRGLKRAEGFELIKELADKLGAEIGASRAAVDSGWIEHSHQVGQTGT
                     TVRPELYIACGISGAIQHLAGMSDSKYIVAINKDAKAPIFSICDYGIVGDLYEIIPEM
                     IESLNR"
     gene            23601..24977
                     /locus_tag="PC700_001230"
     CDS             23601..24977
                     /locus_tag="PC700_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HDF2580546.1"
                     /translation="MKEQWYKDIFARILSMTDDGFIVVNTSGVIIDINDKYCDFLGKE
                     RKDIIGQNIQSIIPNTKMLDVMKDKYCEEGAIHHYSGGNTKEKSVIVSRSYVENDNGE
                     VVAGVAQVKFRLQSFDVAKKLMSEYMELQYYKEQFKDNCGFDKLIGENRDFIELKKTG
                     VKASKTNFPVLLTGETGTGKEVFARAIHNNSSRSDKPMVSINCAAIPEELLESELFGY
                     DEGAFTGAKKGGKKGKFLVANNGTIFLDEIGDMPLTMQAKLLRVLQESEIEPVGGLKT
                     IKIDVRVISATRKNLSKMVEEGLFREDLYYRLNVINIHMMELKDRQDDILLLANYILN
                     KLNVEYKELKVLSDKVKNCFINYTWPGNIRELQNVIKSAYAVSDDMVIMMCDLPSKMD
                     NISRVAQCNVDSNCSIHEMVENYEKSLIIDVLRKYNWKCSKAAEVMGIHKSLLYKKIK
                     KYEIELNN"
     gene            complement(25309..25653)
                     /locus_tag="PC700_001231"
     CDS             complement(25309..25653)
                     /locus_tag="PC700_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580547.1"
                     /translation="MDQIKRLHELQQKSYLTKEEFEELKNNTYIERFELRDECDNSYI
                     YTFYTNDKCNDIKFIKSQFIVTLIKIGFLCECGGVFRQKKIVDEYDYGYNAIYKCDSC
                     GKELDKEVENYD"
BASE COUNT         9793 a         3042 c         4836 g         8249 t
ORIGIN      
        1 ctaatatatt tggtctgtag gttaaataaa agtaccttta gaccaaatat attattaata
       61 acttaataac atcttaaaac ttatttattt atattattta caacatccat ttaataaata
      121 caccataacc tacagctggg tcattttcaa ttgcatgtga cactgctccg attattttat
      181 tatcttgaac tattggagta ccactcatac cttgtacaat accaccagtt tgagctaaaa
      241 gctttttatc tgtaatttta attttaaaac ttttactttc tggatgtttt tggttttcta
      301 tatctataat ttcaatatta tattttttac acttattaaa gttatcttgt aaaattattt
      361 gagcttttcc taatttaact tcatctaaag atgctacttt atattttttt aaattggaaa
      421 tatcaaagtt attaatttga cctcttattc caaaatcagt attttctgta aagctaccaa
      481 taatattttc tcgttttgca tttatacatc caacactacc tttataagat ttttctatat
      541 taaggtgctt agttgtagaa attgaacctg ttttaatagg tatttttctt gcattaccaa
      601 cacttattgg atgacctaca gctccaaact ctgatgtatc tggattgata tacgttaaag
      661 tagcaaatcc agaaagtaaa ttgttaaaac taattttttc taatatgtgt ttggtagttt
      721 tcattgttat aagttgtcca cctctattta ataaaataga tacttgtttt tcttctggta
      781 aagcatttaa ggtattggca aagtcactat atccatcaac ttcagaatta tttaccttta
      841 ctatagcatc acccaatcta aatggagatg cttctccagg atttctaact atgatatttt
      901 ttaattctgc gtcaatttga agtacgtttc caattggtat taaatattca ggttcatttt
      961 tttgtaaatt ttgtgcataa attaaattat ttgagaaaaa atataataaa aataaaaatg
     1021 ttaaaacaat tgtaaatttt gacttagcta agttggtttt tttacttaaa gtattatttt
     1081 taaaatgaaa atttttaagt tgcataaaac acctctttct tattaaatta ttttaataat
     1141 agaattgaag ttttcttgct gcttctatat tatgttgtgc aagaattatg ttttgatgct
     1201 tggaaaactg actcaataac accttagttt tcataaaaat aaataaaata aatagtgtat
     1261 atgataaaat aaatacatat tcatataaat aaaaataaac aataactgtc tagcataaga
     1321 aaggagcaaa aaatgaaaaa atatagattg ttagttactt gtataattgc catgggaatc
     1381 ataattatag gaacatcaaa tccagttagc aatttttaca agactacaaa tttgattagt
     1441 tcaggaaata tcacaaatga aaaagcagat aaagatgaaa attcaaacaa aaataataaa
     1501 gataaaaatg aagacaaatc taattctaaa agtaataaag ataagtcatc agaggacaaa
     1561 tcgtcagata aaaaaaataa aattcaaaaa aataaaaaac ttttaatttg tatagaccca
     1621 gggcatcaag gaaaaggaga tagcaattta gaaccagtag ctccaggttc ttcctctaaa
     1681 aaagcgagag tatcttcagg aacagagggt attgctacaa aaaaaccaga atatgttcta
     1741 aacttagaag catctcttgt tttaaagagt atattagaat ctaaaggtta taatgtaata
     1801 atgactagag agacacatga tgtaaatata agtaattcag agagagcaat acttgctaat
     1861 gataaaaaag cagatatggt tgtaagaata catgcagata gtttaaataa ttcttcaaaa
     1921 actggagcat ctatattgat tcctgaaaaa gatggaaaat atacagctcc tatatatgaa
     1981 gagagtaata aatgtgctga atttatcaaa cagaatatgg aacaatcagg tatacaaatc
     2041 aatggaatcg ttcaaagagg tgatttgact ggatttaact ggtcaaaagt accagccgta
     2101 ttagtagaaa tgggatttat gagtaactat aatgaagacc aaatgatgtc aaatccagac
     2161 taccaaagaa aaatgatgca gtgtatagct gatggattgg atgcgtattt taaataagat
     2221 atgaatagag aaaaagttag gttggtatta ttttcctaat ttttatctct attttttatt
     2281 gtacataatg caaagaagaa gtttatttta ggttaaattg tcgtaaatat aactaaaaat
     2341 attgggaata cttgatttaa gtttaaaaag aatatattat agtatagaat gtgtaaaatt
     2401 tttttgagag gtgttgaaat gctagtagta gaaaatgtga gtcatggatt tggtgcgaga
     2461 acaatattag aaaatgtatc ctttagactt agaaaaggtg aacatattgc actagttggt
     2521 gctaatggag aaggaaaatc gagtttttta aatataataa caaagaagct tatgccagat
     2581 gctggaaata taaaatggtc ttcaagagct acagtaggtt acttagacca acatacggta
     2641 ttaagcaagg aaaaaactat aagagaagtg cttagagagg cttttaagca tatgtttgac
     2701 ttagagcaag aaatgattgc tatgtatgac aaaatgggag aagctagtga tgatgaaatg
     2761 agtaagcttc ttgaagaaac agcagagata caaactatac ttgaaaatag tggtttttat
     2821 atgatagatg caaagataca agaagttgca aatggtcttg gtcttggaga aattggtctt
     2881 gataaagatg taactgactt aagtggaggg caaagaacaa aagtacttct tactaaactt
     2941 ctacttgaaa atccaactat tcttatattg gatgagccta caaactattt agacgaagag
     3001 catataacat ggcttactaa gtaccttcaa gagtatgaga atagttttgt cttagtttct
     3061 catgatattg aatttataaa taatacatgt aatgtaatat atcatatgga aaatggagag
     3121 ttaaatagat ataaaggaaa ttatgatgaa tttgtaagac ttaatgatat aaagaaacgt
     3181 caagaagaac aagcttatga taaacaagtt gaagaaagaa aaagactaga ggattttgta
     3241 gctagaaata aagctagagt agcaactaga ggaatggcta acagtagaca aaagcaactt
     3301 gataagatgg aaatattaga aagaccaaaa gaaaaaataa aacctacttt tgcttttaaa
     3361 gatgctagag cagctagtaa aataattttt gaaacagaga atttagtttt agggtatgat
     3421 gaagcattga caaaaccact taatttccat ttagaaagag gaaaaaagat tgccttaaaa
     3481 ggaatgaatg gtattgggaa atctactcta ttaaaaactt tacttggaat aataaaacca
     3541 tttgaaggaa atgttaaatt aggtgactat ttggaagttg gatattttga acaagagagt
     3601 tcaagggaaa atagtaatac acctatggat gaagtttgga gtgaatttcc agggcttaca
     3661 aactttgaag ttagacaggc tcttgcaaaa tgtggactta caaatgaaca tataacgagt
     3721 caaatgagag tattaagtgg tggagaagct gctaaggtta gactttgcaa agtaatgtta
     3781 aaaaatataa atttcttagt attagatgag cctacaaatc atttagatgt tgaggcaaaa
     3841 gatgaattga agaaggctat caaagagttt aagggaacag ttcttttagt atgccatgag
     3901 ccagaatttt attcagaaat tgttgatgat gtatggaata tagaagattt taccactaaa
     3961 atcgtgtagt gatttaactt attgctgggg ggatttcatg aaaaagtttc taaatgtact
     4021 tttttcagtt tgtattagta ttgttataat agtaggtgtt attaatttta ctgttggatt
     4081 taaacaactt tattattttg acattgatta cttaaatata tcagagttat ctggtttatc
     4141 tgaagatgat ataaaattga actacgatta tctaattgat tataacttaa ataagaatgt
     4201 gtctgagttc aagttaccaa cattaaaatc ttctccacaa gggaagatac attttgaaga
     4261 agtcaggaat atatttcaaa atataaataa attagctaaa ttattattgg tagttttttt
     4321 agtaggaatt atattgagtg taaaaaataa aaatataaaa atcttgaaaa ctacatcaat
     4381 aacacttata attatgccat tattattaac agttccaatt ttattaaatt ttgaaaaatc
     4441 atttattatt tttcataaat tattatttag aaatgattat tggatattta atccaaattt
     4501 agacccagtt ataaatatat tgccagagga attttttttt cactcaggaa tgatgatttt
     4561 aatattaatt ttgttagcaa gtatcttact atttgtaatg tatagattat ataaatcttt
     4621 caaaataaaa taagatagca aaaacgaatt atattaaagg ttatacaata aaaatagcta
     4681 tgtaataata tttaaactaa gagtagtcga taaatattta tgcatagcta tttctaatag
     4741 aaattacttt ttactagtga ttttaactaa atcttgaaat ttgcccataa tgtaattgta
     4801 attatctttt ttgtgaggta acatgcaact actgcaatct ttgatacctt tatctgtata
     4861 cttaaagttt ccaccacagt tttctcctaa agcatacaaa ggacaatagc aaaatagaca
     4921 gttaaactct tctggtttat tagttttatg acaagggaaa aattcgcaat ctttgtgatt
     4981 aaaaaactta taattttcgc ccatattttc ctcctaatta aataaaattt aaatttttta
     5041 tactatataa gattttggtg ataatataat tatactaaat aattttatga atagtcgata
     5101 atatagtatt attaatcata gtattaatgg tataattagt caacagaaaa ataatgtaaa
     5161 aattatgtaa gtgtattttg taattagaat atgttttata acaaaaaatg aggtgattaa
     5221 gtgaaagaaa ttcaagaaaa agcacttctt gtaggtctga atttgactac aatagtgaaa
     5281 aaaaatgatg atatagacac tagtgaatct atggaagaat taaaagagct aacaaaagct
     5341 gctggggcag aagttgtagg tagcttaatt caaaataaac attctgtaga tgcagcttat
     5401 tatataggaa aaggtaaagt agaagaaata agagcctata gtgactcttt agatgctact
     5461 ttagtaattt ttaatgatga attatctggt gctcaaatca gaaatataga gaatgttgtt
     5521 ggaagaaaag taatagatag aactacttta attttagata tatttgctca aagagcactt
     5581 agtaaagaag gtaaattaca agttgaatta gcacaattaa aatatagatt gccacgactt
     5641 tatggaatgg gtggtgagat gagtagaaca ggtgcaggga ttggtactag aggtcctgga
     5701 gaacaaaaac tagaaataga taagagacat atacttaata aagcagcaga cataaggcgt
     5761 gaacttaaag aagtaaaaaa gaatagggag acacagagag ttaagcgttt aaagtcaaat
     5821 ataccgattg tagcacttgt tgggtataca aatgcaggaa aatctactct tttgaacgaa
     5881 ttaataaaga cacacaagga ttatgaacaa gaaaaagaag tttttgtaaa agatatgctt
     5941 tttgcaactt tggatgtaac tctaagaaag gctcttttgc cgaataaaaa agaattttta
     6001 gtagtagata ctgttggatt tgtaagtaaa cttccccatg atttagtaga ggcgtttaaa
     6061 gcaacattgg aagaagtaca gtatgcagat ttaattttac atgtaattga tgcgacaaat
     6121 actagttatg aattgcaaaa aagtacgact gaaggtgtat taaaagaact tggtgtaaat
     6181 gataaaaaac atatacttgt atataataaa gtggataagc tagaattgga tatatatcct
     6241 aaaagtcaag aagatattgt gtatatttct gcaaaacaag gcataaatat ggataagcta
     6301 ttaaatatga tagaaattgc tttaatggaa aatacttatt cagtaagctt gatgttacct
     6361 tatgagagag gtgatatatt tagtagaata aaagataaat ataatgtgga gaattttgag
     6421 tatggggaaa atggcataac gctagatgta aacttcgatg aagaagattt taatatctat
     6481 agagagtaca tactagaaaa ataggttgaa tatggatata aattgggaaa atatagataa
     6541 tcttgaagat tattttataa cctatcttct ttataaagaa tctaaaacag tatctcaaat
     6601 tagtaaaatt agaaatattt caagtactga agcaggtgag caacttatac aagcaaaatt
     6661 aaaaataaag gaaatgcaaa aagatgattt tgaggcatct aaggatattt tagataaatt
     6721 tttagaacta gataaaatta agagacttga ttttatggat agcttagatg acgaaaaaat
     6781 ggtatacttt aagagaaagg ttttcaaaag aatccttgtg gaaaaaaatg ctgaagattt
     6841 aattgtttta atttgggcga caggtgagct aaaagatgat agattcttaa agttgcttca
     6901 tcagctcaca aaccacagac actcagatat tagaagaata acttattcag caatcagaaa
     6961 gattgaatca ccaagtagta gagaagtact gcaaaaaggt ctctacgata aaaatgcaca
     7021 aactagacag tattgtgcaa aggctctttc aaaactaggg gatgaaaaca gtttaaagat
     7081 tttacagcag ttgaaagaca agaataagaa ttttgaaaaa gaatatgtgc ttagagctta
     7141 tgacgaagcc attaagtcat tggaaaatgc aaaaaatttg aaaaagtaat tttttattaa
     7201 agcaatttgt ttacttaatg atgtatttct agagtttaac aaggtaataa ataaattaat
     7261 gggaggggtt tcaaatgata gtaagacgtt gtgcaggtgg agtagtgttt tatgctaata
     7321 aagtacttat tgtaaaaaat gataggggag aatggacact tccaaaagga aagatacttg
     7381 gaggaggact tccatatgag agtgctgttc aaagagttaa agtagaaact ggcatagatg
     7441 ctaaaatgat agatgtggct ggcgatacta tgtatgaatt tttctcaaga agtagacaac
     7501 aagaagtttg taatgcaata atgtggtatg ttatggaagc ttgtaacact aattgtgtac
     7561 ttgctcctga atttcaagaa ggtggatttt ataaagtaaa ggatgcttta gatatgttat
     7621 cacatcataa agagcaagct ttagtagaag tatcttataa aaagtttaag gaattaaaga
     7681 aattagtttc agagggcgat gatgttccag caaattaatt gtgaatttag aggattctga
     7741 ggaagggata gccccttcct tttttaattg aagcagtttt aagcgtaaat tatatagtct
     7801 ctttaaatta tgatttatca atttagagct ttagtagttt atatttaaaa tcagtaagat
     7861 atatttaaaa taaaaattaa aaagtataca attttattat ataattgtct tatgatattg
     7921 attaaagaag tattaaaagt ccatttattt ttaaatagaa taattttata gatatataat
     7981 ttgatgtgta tattagaaaa tgattaaaat aggaggaaat acaatgagca attacaggac
     8041 attacatgaa tttggaacag atgaaataac tattgaaaag tctgttttta ttggatatgc
     8101 taagcctata aagagtgaag aagaagcatt agagtttata aatgaaataa agaaaaagca
     8161 taaagatgca actcacaatg tgtgggctta tactgtaggt aaaaatatga atatacaaag
     8221 atatagtgat gatggagaac cacaaggaac agctggaata cctacattag aggttataaa
     8281 aaaagaagat ttgagagatg tagtggttgt tgttactagg tattttggag gaacaaaatt
     8341 aggtgcaggc ggtctagtta gagcttatac aaaaggtgct aaattgggac ttgaagcagg
     8401 taagataatt tataaggtaa tgtatcaaga agttaaggtt aagatagact atactcaact
     8461 tggaaaagtg caaaatgagt taatgaattt ggggtatttt ataaaagata ctgtatatga
     8521 ggataatgtt gagatagtag tgtattcaag attagaagat gtagagaaat tatcagaaaa
     8581 aatgattgat ataacaaatg gaacaggaaa aataatttta ggagaggaat tttatttgtc
     8641 tgaacaaaat ggagaaattt taatataatc taaaaggtta ttagaatagt taatacctca
     8701 aatagccaat tagtattttt aagtgaaaag ccattaaatt gatttaatct aaatcaattt
     8761 aatggctttt tatcggattt tgcacagaat ccttttttta cttcaaataa accgactaaa
     8821 agtaaaaata tcccaaatat tttttttaat atatctgaag acagataatt agctaatagt
     8881 gaaccaaata cagctcctaa aaccccaaac acagctatag gtataaccaa tttaaaaatt
     8941 acatttttat tttttatatg tatagcaagt gctataagtg tcatagggat agatgaaagt
     9001 aaatttacgc tttgtgctat tttagtgtct atgccagcaa gtaaaacgag tgcaggtata
     9061 aggattgttc ctccacccat tcccattcct ccaataattc ctgcaaaaaa tccaattaga
     9121 gcaaataaca ttaaaacacc atccttaatg ctgcaattat cataattatt ccaaaagaaa
     9181 ttctaagaaa ctttccagta accttattta gtaatttagc tcctataata ccaccaagaa
     9241 tacttccaac tgcaacttta attgttaagt taacatcata agtaccttta gatacatata
     9301 aaacagaact tgctgtcgtt aagaatatta ttatagaaag agcagttgca tgagatttat
     9361 gttcttctac cttaacgatg tcatttaata ttggaactaa aagagtacct cctccagaac
     9421 caaaaactcc atttataaat cctgtaaaaa ctccaattat cgtatttttt aagttaaaat
     9481 caaagtattt tttctttaaa tcatcagttt ttttcataaa aatcaccttc tgaaatagtt
     9541 ttaccaaaat aggcaaatta tatttatata aaataagatt aaatataatc tttcatgttt
     9601 gtttaatttt gcataaaaaa aaacatatta aaacaacata ttaatatatg gtataatata
     9661 ttaaaacaaa gtagcaaatt tgagaggaga taacaatgtc aaatataaaa atacaaatag
     9721 acaaaactgt agaatggctt ataaataagg ttaatgaggc aaatgcaaaa ggattaattg
     9781 ttggtgtatc tggaggaatt gattcggcag tagttgctaa tttaattaaa aaggcatttc
     9841 cagaaaactc tatgggagtt ataatgagca taaaaagtaa tcctcaagat agagaagatg
     9901 cacttaaggt tatagaggga tgtgatatag aataccttga tttagacctt atagaaccac
     9961 aaagtgctat attagacatg gtagtaggaa atttaaaaga taaacattta tatagagcag
    10021 aatatttaaa gatgacagat gcgaacttaa gagcaagagt aagaatgagt acaatttata
    10081 caattgctaa caatctagga tatttagttg ttggtacaga caatgctgca gaaatacaca
    10141 caggatattt tactaaattt ggtgatggtg gagttgatat tttacctatt gcaaatttaa
    10201 ctaaaggaga agtatatgaa tgggcaaaag agcttggtgt tcatgaagat ttaataaaca
    10261 aagctccttc agcaggtctt tgggaaggtc aaacagatga agatgaaatg ggaactactt
    10321 acaatatgat agatgctgtg ttagaaggta gattggatga agttcctaaa agagaccaag
    10381 agataataga aagacttcat agattaagtg aacacaagag aaaaacgcct gcacaacctc
    10441 ctaagttcta atttactatt tataagattt gtgataaaat ttacaagtga tgaaaattag
    10501 aataattaat tgtacgtatt aaggagtgga aatatgggac gtataggtaa tataattaat
    10561 agaaaaggta aacaagatgc tcaaagagca aagatattta ctaaacatgc tagagcaata
    10621 gcagttgcag ctaaagaagg tggagcagac ccagagtaca atgcagcgtt aaaaacagct
    10681 atagaaaaag ctaaggcaga taatatgcca aatgataata tagatagagc tatcgctaaa
    10741 ggtgctggag caggcgctgg agaagactat gaaactatag tttatgaagg atatggacct
    10801 ggtggagttg ctgttatagt agaaacttta actgacaaca aaaacagaac tgctggtaac
    10861 gtaagatact attttgacaa aaacggagga aacttaggaa ctagtggatg tgtgtctttt
    10921 atgtttgata aaaaaggaca aatattagtt ggtttaggag atggagtttc agaagaagaa
    10981 ttgatggatg ttgcattaga agcaggagct gaagatttca taacagaaga agatggttat
    11041 gaaataataa ctacaccaga agatttttct agtgttcgtg atgagttaaa agctaaagga
    11101 tatgagttta tatctgctga tgtgaagatg atacctcaaa ctacgactgt attgactgaa
    11161 gaatctcatt taaaaatgat gaataaatta gttgatatgc ttgaagaaga tgatgatgtt
    11221 caagatatat atcataactg ggaagtagaa taaatagtat aagcatattg aaatttataa
    11281 gcattgaatc aattttaaca tgtgataaat tttatagaat aagaaattac taaaatagac
    11341 atacgcacct ctaacctata tttgcataat aatatgtaag tataggtaag gggtgttttt
    11401 atatttatag gttaagaaat ttaaagtaaa ttttgagctg gacttgattg tctataaata
    11461 attgatgggt atataacttg ttgctgaata ccatttatac gcccagttac tgtatcaata
    11521 atcatcctaa gactacgttc tgctgtctct tccattttta aatctataac tgtcaaagga
    11581 ggaatacttc ttgccattag tcgtgaaggt ccattacttg ctgcaaatat ttgaatatcc
    11641 tctccaatta caattccaga ttcatgaaat gcattcatca taccaagagc tacaacatca
    11701 tgattacaaa aaattacttt ttttagtgaa ttttttgcaa gcattttttg agcaatatca
    11761 tatccactat ctattttatt agaacaataa tatatagaat ctgatagatg tattccatgt
    11821 tctttacatg tttttaaaaa agatttaccc cttaaagtca tacatgtaaa ggaagttggg
    11881 ttgaatacaa cagcaatatc atttttacca ttttgaatag catgtaaagc tgctatacga
    11941 ccagtttcct cattatcaat agatacactt gaaaaaccag gtatttcacg atttacaaca
    12001 actacaggaa ttttaggttt attttttact agatattcta tatcatcagc attgatacct
    12061 gttaaaacaa ttccatcata ataattattt gcaaaaaagt attcatcatc acatagatga
    12121 ccaactttgt aaggtcgtat gatgatattt atgtttaaat gttctaaaga aattatgtgg
    12181 tttattgctt gaagtatatt ggttgatgcc aactcaaatc cctcatcctg ccaataaaaa
    12241 gctattttaa tattattatg tttaatagga gtagagcgtt tatatccaag cttatcagct
    12301 gaatttaata taagctcttg tgtatgtagc ggaatcctgc gttcatttcc tttaccactt
    12361 aagacgtacg agacagttgc tgaagaaaga ccacagtctt ttgcaatatc tttgattgtt
    12421 gccatgataa aatcctcctt aaatagttgc ttaaatttgc ttataccaat aatcttaaca
    12481 tatattcaac taaagaacaa tattctacaa ggtgcatatt ttacaggatt agaataaata
    12541 aatttaaaaa aacttcttac ttatgttgac ataatttagt ttataggatt ataatatgct
    12601 taaataagct tattattaac tcaaattaag caaagttaag caggaacatg caatgctagc
    12661 ttgcaatgtg actgtaaagg gggaaaaggt atgagtaagg taaatatatt tgaagttggt
    12721 cctagagatg gatttcaaaa cctgaaagag tttttagaaa ctgataaaaa gcttaagatt
    12781 attgatggtc ttattgcttc agggattaaa caacttgaaa ttagttcatt tgtaagccca
    12841 aaagcaatac ctcagatgaa agatgcaaaa gaagtagcaa cttattgtgt agaaaaatat
    12901 cctgatgtga aactttatgc attagttcca aatttatatg gtgctaaagt tgcatgggag
    12961 tgtggtataa gagatatatc ctatgttata tctttgagtg aaactcataa taaaaaaaat
    13021 ataaatcgta cccatgaaga gtctattgat gagcttaaaa aaataatgaa tacttatcca
    13081 gacatgaata tatgtattgg aatggctact gtatttgggt gtccttttga aggtttacca
    13141 aaaattgaag atgccataag atttgctaaa cgcctttggg aaaacggtat cagggagata
    13201 aatctagcag atacaatagg gattgctgac cctaagcaag taagagaaac tattagtgca
    13261 ttagttgaag ctttaccaga gtgcagatgg caggtacata ttcatgatac tagaaatatg
    13321 ggaatggtta atactcttgt ggctattgaa tctggaatta ctactgttca gtctacactt
    13381 ggaggattag gaggatgccc atttgctcct ggagcatctg gcaatactgc aactgaagat
    13441 ttagtatata tgcttgaacg tatggggtat gatacaggag ttgattttgt taaacttctt
    13501 gaattatcaa agtatcagaa gagttttgta aatggagtat atagtggtca tcatgtaaat
    13561 ataaagtctg atagttgtaa ttgttagttc tattgattaa tcgattaaaa aaatatgttg
    13621 attaaaaaaa tatgctgatt ggtaaaatag atttattgat aaaaaagata aattataaaa
    13681 tacattatct aataaaacaa ataaaaaaat tgagggggat tagtatggat attatagctt
    13741 tacttttatt tttaggagtt attattattg catttgtacg taagaataat gcaggtattt
    13801 tggctctagc tattggtgtc atagctgtta gaatttttgg aatggatgat aaagaacttt
    13861 atggagctat aagcaactca atgtttacta cacttgttgg tataacacta ctctttgcaa
    13921 tagtgaactc aacaggagca ttaaagcttg ttgcagaaaa aattgtagct gcttctggta
    13981 aacgtgtttg gcttatacct atagcagttt ttatagcagg atttgttgtt gctggtgttg
    14041 gacctggggc aattccagca cttgcaatta tcccagcaat ggctgttcct gtagccataa
    14101 cggttggtta taatcctgta atgcttgcac ttataggtga atgtggatta atggcaggtc
    14161 gtatgactcc tattactcca gaaggtcaaa ttataacaac cgttgcagaa ggggttggaa
    14221 ttgaaaatgt tatgcctact atcttagcct gtcaaactct ttctacaatt gtattttcaa
    14281 tagtattgtt tataattttc aaaggatata aacttaaaaa acctataaat gttatagata
    14341 gtaagcaaat agaaaaattt aatataaagc agataatttc tctaataagc atagtagtta
    14401 tgattgcttt tatcataatt tttgatatga atgttgggct tgcagctttt gcagtttcag
    14461 ctgtacttat ttttttcaat attgctgatg aaggagaatg tatcaaatca attccatgga
    14521 gcacgatagt aatggttctt ggagtaggtg caatgatgac aatcgtagat gcagcaggag
    14581 gtattgactt gatgagtaat gcattatcat cactgatgaa tagtaaaaca gctactccaa
    14641 ttatgagtat ttcagcagga cttatgtcta tggttagttc tgctttagca gtagtttatc
    14701 caactatgat gcctatgtgt gttgatatag ctaaggcagt tggaggagta gacccactag
    14761 cacttattgc tgcagtcggt gttggaggtt ctcttgctgg tgtaagccca ctttctactg
    14821 gtggggcatt gattttagca gctatgggtt caagtaagaa agactttaat aaggaagaac
    14881 aaaataaggt ttttgtacag cttttcatta tgagtgcagt tggcttattg gttattgcag
    14941 tagtatcaat attatcattt aatgcaataa cacatttaat gaactaatta taataactga
    15001 aaataattca ttaaatttaa tttttaatat taatcaatat aatataattt ggaggggaag
    15061 ttatgaaaaa aggagcatta agcaatataa aggtacttga ccttacaaga gttcttgcag
    15121 gtccatattg tacaatgatg ttggcggatt ttggagctga tgttataaaa atagagatgc
    15181 caagaaaagg tgatgataca agaggttttg gaccttttgt aaatggagag agtttatact
    15241 atgcaaatat aaatagaaat aaaaaaggta tcactctaaa ccttaaatca gaagaaggaa
    15301 aaaatatatt tttagaaatg gtaaaacatg ctgatgttgt agtagaaaat tacagaccag
    15361 gtgtaatgga aaagttaggt ctgggttatg atgagttggc taaagttaac aatcaaataa
    15421 tttatggagc tgtatcagga tttggatgtt atggaccata ttcagaacgt ccaggttatg
    15481 atattattgc acaggcaatg ggtggtctta tgagtataac agggcaagaa ggaggagaac
    15541 caacgagagt tggtaatgct atgggtgatg tactaggtgg aatgaattta actattggta
    15601 tactcacagc actcaacgct agaaaaataa taggaaaagg acaaagagta gatgtttccc
    15661 tcgttgattc tgttgtatcg tctctcgaaa ctggtataca gagatacttt gattcaggac
    15721 aacctgaact tatgggtaat agatatgctt cagcctatcc ttatgactct tttaaggcaa
    15781 gtgatggaag ttttgtaata ggctgtggta atgacaaact ttttgaaaag ttatgtaaaa
    15841 aagttctttt tagagaagac cttttaatag atgagagatt taaaacaaat cttaagcgtt
    15901 gtgaacatta caaggctctt aaaccagaaa tagaaaaatg gtctgttaag catacaataa
    15961 aagaagcagt tgacttaata tcagaggctg gagttcctgc tgctcctatc tataatcttg
    16021 attgtgttac aacagatgaa catatagcaa aagcaagaga aatgattgta gatattagtc
    16081 atcctattat aggaaatatg aaagtcaatg gaaatccagt taagttgatg acaacaaaag
    16141 ctaaaattga tatgccagca ccatctctag gggaaagtaa cgaggagatt tataatggtt
    16201 ggcttggttt tgatagtgat aaaataaaag atttaaaaga aaatggagtt atttgattaa
    16261 aaaatagcta aacacacttg tgactttagt catgggaggt tcagttagaa taatatcaaa
    16321 aaatattaaa tcaatactgt aaatcaaata tagaattgtc agaagcattt aagatgggaa
    16381 aagctactta gacattgagt ctttgtggct tttcctattt tcattaagaa ctttatatta
    16441 tcagaataga tatgtactaa actaaagtat taacaattta aattaataga aaataaacta
    16501 cctaattatg ttgaataaag actactttag tgaaaataaa cttatagaga tgctatttaa
    16561 attattttaa tggaaatatc aaaaatgaga ctgcattctc aaaaatgaga ctgcgttttt
    16621 taagtatttt ctcgaaaatg agacaaaaat attgttttat gagttttatt gaagaaaaat
    16681 tcttctgtga attacaattt aatcaatttt aaagttgaaa attattaaaa atttagtgaa
    16741 atttatgtat atttcgttta ttttattaaa aataacaatt ttttactaaa aataaagaac
    16801 ttcaatggtg attggcatag tacttgctat tttatattaa caagaaatgt agaaaaaata
    16861 acaatgtata atggggggaa tatgatggaa tctgtaatgg aaaatttgaa caatcttaag
    16921 gtggaggtta aggacaaagt ctgtgttatt actataaata gacctaaagc tttaaatgct
    16981 cttaatagtg acactcttag ggaactatca caagttattg atgtagtatc agaaaatgag
    17041 gcaatattag gagttattat tactggagaa ggtaaagttt ttgtagccgg tgctgatata
    17101 cgccagatgc aaaactataa aagtgaggaa ggcagaaaat atgcaggtta tgctcaggga
    17161 atttttgata aaattgaagc tcttgagaaa acagtaatag cagcagttaa tggatatgca
    17221 ttaggtggtg gatgtgaact tgctatgagt tgtgatatta gaattgcatc agaaaaggct
    17281 atatttggac aacctgaagt taatttagga gttattccat gttttggtgg aactcaaaga
    17341 ttatcaagac ttgttggaac tggaatagct aaagagttaa tttttacggg gagacaagta
    17401 aatgcagaag aagctaaatc tattgggctt attaataaag tggttccaag tgatttactt
    17461 ttagaagagt ctatgaagat gatgaaccaa atagttgaaa aagcgccaat agcaataaga
    17521 tatgcaaaag ttgttattaa caaagggata gatatggatt taaagaatgc tcttgaacta
    17581 gagaaagata ttgcaggttt aacttttgct acaagagata agcaagaagg aatgaatgca
    17641 tttatagaaa agagaaagcc agtttttgaa aataagtagc tattataact aaattggaac
    17701 aatgtagaat atattcaaaa tctaaatttc gtgaaaaaat acatatagtt taattttaaa
    17761 aggagttaat ttatgaatac gggagtaatt ataagtttag tagggattat catagctcta
    17821 tctcttctag ttgtaatggt tatgaaggga ataaatattt ttattatagc aattgtatgt
    17881 tcatcattag ttgctataac aggaagccta aatgtgtatg atgcacttaa agtggattat
    17941 atgacaggat ttgttggatt tcttcaggca aatttcttta ttttcctaac agggacatta
    18001 atgggaaaaa taatggagat tactggtgga gcaaaatcta ttgctaagat gattgtaaga
    18061 tggattggta aggataaagc actactttct attccaatag cttgtggaat attagcctat
    18121 ggtggagtaa gtgttttcgt tgtaagtttt gcagtgtttc cgatagcttt agaaattttt
    18181 aaagaagctg atttgccacg aagatttata ccagcagctt tgacttttgg atgctcaact
    18241 tttgctatgg ttgcaccagg ggcaccacaa attcagaata ttgttccagc atcaacatta
    18301 gggacagata ttatggccgg agcagtaaat ggttttatat catgtgcagt aatgtttata
    18361 gttggaagta ccattttgta caaaatggtt tcaaaagaaa aagccaatgg tggtcatttt
    18421 atagcacatg aatcagatat atttgatgaa gcagaagcaa ctaattctaa gagagaaaat
    18481 ggtcctaatg gtgtaattgc cttaattcca ttgatagtgt caattttaat aattaatgta
    18541 aaagttaatg gaaaagcgat agtaccaatt gaggtgggag tatttattgg agctttatta
    18601 gtatatttat tgttaaataa atatcaagat aatactaaaa tagttggaca tgttggtgat
    18661 gcatgtaaaa ctacagtagt agcaatatgt aatacttgtg ctgtagttgc atttggttca
    18721 gtagtaaaat cagcagttgg ttttgacttc atagttaatg ccatgactaa tattcctggt
    18781 cctcctattg caggggctgc agttggaact acagttattg caggtatttg tgggtcagct
    18841 tcaggtggac taggaattgc agctccactt cttggaccag tatttttagc caagggtgtt
    18901 tccgctgcag cattaactag gactatggca atttcatcag cagcattaga ttcacttcca
    18961 cacaatggat atattgttac tgtaacaaat ggtctgtgta aagaaacaca taaggatgcc
    19021 tatatgccaa tattctgggt aactgtttta actccgttta ttggaaccat agtagcagtc
    19081 ctactgttta caatgttccc gggattgcca taagagaaaa gataaaaggt ataaattata
    19141 ttttgatatt ataaaatcaa ttttcaaaat atatacaata aatttcatgt tttaatacta
    19201 taaatatgta tttaaaataa caaattgacc atataaggag gaattgaaag tgttaaataa
    19261 accattagag ggagttaagg ttattgacct aacgtatttt gtagcaggtc cagggacttc
    19321 taaaatatta gctgattggg gtgctgacgt tattaaggta gagccaagct ttggagaccc
    19381 aggaagaaag acaggggcaa caatgacaat gccaatagat gattataata atccttttta
    19441 cagcacatat aattcaaata aaagaggttt gtctataaat cttaagagtg aaactggaat
    19501 tgaaattatg gataagcttt taagtgaagc aaacgtattt gtttcaagtt atagaacagg
    19561 agcattaaaa agactaggtc ttgattatga atcattaagt aaaaagcatc cacaccttat
    19621 ctggggtcag ataaatggtt ttggcgattt tggacctgct aaggataatg caggatttga
    19681 tacagttgcc ttttgggcaa gaagtggggc tatgcttgat atagcagaaa aagatacaag
    19741 tccaataaat ccacctatag cctttggaga tgcaacaact tcttgtagtt tatctggagg
    19801 aatatgtgca gctttgtacc agcaagctaa gactggaaaa ggacaaaaag taatggcatc
    19861 tttatttggg caagcaatat ggaatgcatc atctttatta gcatctacac agtttagtga
    19921 tgagtatcca aaaactagaa aaaatgctat ttcaccagta ataaattctt atcgttgtaa
    19981 ggatggaaag tggatattct taagtatttt agagcatgaa agatattttg aggcactttg
    20041 tagattattt ggaagagaag acttaataaa agaagagaaa tttgcatctt cagtagaaag
    20101 taagaaaaat gctacagaac ttattaatat tttggatggt gaatttgcaa agtttactca
    20161 aaatgagatg gttgaaaaac ttactgctgc tgatatagct catgaaaaaa ttcaacatgt
    20221 taatgatgta gtgactgatg aacaggctat tgctaataac tatatatatg agcatacaag
    20281 taaaaatgga aataagacaa tgcttgcatc cactccagtt aaatttggaa atattgaagt
    20341 taacatgact tgtgatgctc cacttatagg agaacattca gatgagatat tgagagaact
    20401 tggatacagt gattctgata tagaagctct aattgaatct ggaattgtaa caatgaataa
    20461 agaagcatgt gaaatatgtt aataaacatg tgaagtatgt taataaatag gagggaattt
    20521 tatggatttt agattaacag aagcacaatt aatgcttcaa agagtagcaa aagaatttgc
    20581 agagaatgaa atagcaccaa tagctgctga aacagataaa acaggtattt ttccaaggga
    20641 gctttttagc aaaatggcta aaataggatt taatggcatt ggaacacctg tagagtatag
    20701 tggttctgga gggacagata tagaaaaggt aattgttgtt actgagattg ccaaaaaatg
    20761 tgcagcatct gcagcaatat tatcaataca cacaatatat gctcaagcta ttttgaaatt
    20821 tggtactgag gaacaaaaga aaaaatacct tccaatgatg gctgaaggtg ggtgtgtagg
    20881 tgctttcgcc ttaactgaac ctaatgctgg ttcagatgct gcaagagctg caacaactgc
    20941 aattattgat gaagaaacgg atgaatatgt tttaaatgga actaagtgtt ttatttctgg
    21001 tggaggtcaa gcagaatcat taataatatt tgcactgaca gacccatcaa agggaataaa
    21061 gggaatgtct gctatcattg tcgacaaagg tactccaggt ttttcaatag gaaaaattga
    21121 agaaaaaatg ggtattcatg gttcagaaac tgcagagtta atatttgata actgtagagt
    21181 tccaaagtct aacttattag gaaaagaagg caaaggattt aatatagcta tgacttgttt
    21241 agatggagca agaattggaa ttggagctca agctgtagga attgctgaag gggctttaga
    21301 agaaagtatc aaatattcta aagaaagagt ccagtttggc aagccaattt cagcgttgca
    21361 aggtattcaa tggtatatag cagatatggc gacaatggtg gaatcagcaa aattattagt
    21421 atattatgct gcagatttga aagctagagg agaaaaacac actaaagaag ctgctatggc
    21481 aaaatacaat gcgtcaagta ctgcaagaga ggttacaaat ctagcccttc aaatccatgg
    21541 tggatatgga tatatgaagg attatccatt agagagaatg tacagagatg ccaaaataac
    21601 agaaatttac gaaggtactt cagaaataca caaagttgtt atttcaagag cagtactagg
    21661 ataggaggaa aagaatgaat atagttgttt gcttaaagca agttccagat actaatgaag
    21721 ttaaaataaa taaagagaca ggtactctaa taagagatgg tgtaccaagt ataatcaatc
    21781 cagatgatag aaatgcttta gaagaagctc taaaaatgaa agatgaacta ggagcagtga
    21841 ttaaggttat aagcatggga cctcctcaag ctaaaagtgc tttaaaagaa gctttggcaa
    21901 tgggagctga tgaagcttat ttaatctcag atagagcttt tggtggttca gatacttggg
    21961 ctacatctac aattattgct gctgctatag aaaaggttgg aaagtatgat gtaatattct
    22021 gcggaagaca agctatagat ggagatacag ctcaggttgg accagaggtt gcagaatttt
    22081 tagggatacc acaagttact tatgcaaaag aagtaaaggt tcaagatgac aaattactag
    22141 taactcgtta tacagagaca ggggattatt taatagaagc aaagatgcca gttttattaa
    22201 ctgctataaa agagttaaac aatccaagat atccaagtgt aaaaggaatc ttagaagctt
    22261 ataataatgg agatgcgaag attactgtat taacacttgc agatttagat gttgatacaa
    22321 ctcaaattgg attaaagggt tcacctacaa atgtttataa gtcttttgca ccagttaaag
    22381 acaagcataa tgaaataata gaaggtataa ataaaaaaga aaaagctgag aaactaatag
    22441 aaatactatt cgatttgaaa ctagtatagg agtgttaaga tgatgagagc aaaagttaat
    22501 caaggtataa atttaaatga ttataatggt gtttgggtaa taggggagca aagggaaggc
    22561 aaaattaatc ctgttacgat agaacttatt ggtgaaggaa gaaagcttgc tgaccaatta
    22621 ggaaaagagc ttgaagttgt gatagctgga tatgaagtag agaaagaagt gaaggaatta
    22681 cttcactata gtgttgataa agtatattac atcaatgacc ctcttcttaa agatttcact
    22741 actgatggat atgcaatttc aattgcaaat ttaatcgaaa gaaaaaagcc agaggttgta
    22801 ttggttggtg ctacttctat aggaagagat atagcgccta gaattgcagg taaggttgga
    22861 acaggtctaa ctgctgactg tacaaaactt gaaatagatt caacagataa taagttatta
    22921 caaacaagac cagcatttgg tggaaattta atggccacta ttgtttgtcc aaaaaataga
    22981 ccacagatgt caacagttcg tccaggagtt atggcaaaag ctgtaagaaa cgaatcagag
    23041 actggaattt tagaagttgt tactccagaa cttactgaaa aaatgattag aactagatta
    23101 gtagaggtac ttccacaaga aaagaaatct gtaaatctaa ctgatgctag aataatagta
    23161 tcaggaggaa gaggattaaa aagagcagaa ggatttgagc ttataaaaga attggctgat
    23221 aaattggggg cagagattgg tgcttcaaga gcagcagtag attcagggtg gatagaacat
    23281 tctcatcaag tgggacaaac aggaactact gttagaccag aactatacat tgcatgtgga
    23341 atatctggag caatacaaca cttagcaggt atgagtgatt ctaaatatat agttgctata
    23401 aataaagatg caaaagcccc tatttttagt atatgtgact atggaatagt aggagattta
    23461 tatgaaatta tacctgaaat gatagaatca ttaaataggt aacttttaga aaacattgaa
    23521 tatgtagaat gataagtctt ataatattgt attgtaagac ttattatttt tgctgaaggt
    23581 tatagtgggg agatgaaaca atgaaagagc agtggtataa ggatatattt gcaagaatat
    23641 tatctatgac tgatgatggt tttattgttg taaatacaag tggagtaata atagatatta
    23701 atgataaata ttgtgacttt ttaggaaaag aaagaaaaga tataatagga caaaatatac
    23761 aaagtattat accaaataca aaaatgctag atgttatgaa agataagtac tgtgaagaag
    23821 gagctataca tcattattca ggtggaaata caaaagaaaa aagtgtaata gtaagtaggt
    23881 cttatgtaga aaatgacaat ggagaagtag ttgcaggagt agctcaagtt aaatttagat
    23941 tgcaatcatt tgatgtagct aaaaaattaa tgtcagaata tatggagctc caatattata
    24001 aagagcagtt taaggataac tgcggttttg acaagctcat aggtgaaaat agagacttca
    24061 tagaacttaa gaaaacaggt gtgaaagctt ctaaaacaaa ctttccagta cttttaacgg
    24121 gtgaaacagg cacaggtaaa gaagtctttg ctagagcaat tcacaataat agcagtaggt
    24181 cagataaacc tatggttagt ataaactgcg ctgcaatacc agaagaatta ttagagtcag
    24241 agctatttgg ttatgacgaa ggagctttta caggagcaaa aaagggtggt aaaaaaggta
    24301 agtttttagt tgcaaataat ggtacaatct ttttagacga gataggtgac atgcctttga
    24361 ccatgcaagc aaaactttta agggttcttc aggaaagtga gatagaacca gtgggaggtc
    24421 ttaaaactat aaagatagat gtaagagtaa tctctgccac aagaaagaat ttaagtaaaa
    24481 tggttgaaga gggattattt agagaagacc tatactatag attaaatgta ataaacattc
    24541 atatgatgga attgaaggac aggcaagatg atattttatt gctagcaaat tatatattaa
    24601 acaaattaaa tgttgaatac aaggaattga aggttttaag tgataaggtt aaaaattgtt
    24661 ttattaacta tacttggccg ggaaatataa gagaactaca aaatgtaata aaaagtgcct
    24721 atgctgtaag tgatgatatg gtcattatga tgtgtgacct tccatctaag atggataata
    24781 tcagcagagt agctcaatgt aatgtggata gcaattgttc aattcatgaa atggttgaga
    24841 attatgaaaa aagcttaatt attgatgtgt taaggaagta taattggaaa tgttctaagg
    24901 cagcagaggt tatgggaata cacaagagtc ttttatataa gaaaataaaa aagtatgaaa
    24961 ttgaattaaa taattaagat aacttgaatt aaggcaatgc taaaaaaagc gcccttattc
    25021 aaagcgaact gtataaactg ttttatgcag ttaaaacgtg taaaagtaga tgtgaagtac
    25081 taatgtcaat cattagtact ttttattgta tttataatta ctatctatat ttcagtatct
    25141 tttgatatct aagattatta tgtttaattt aggatataac aaaaccctct tagttattga
    25201 cagtttttta tattttaact gtctactaag aggggatagt ttagtttaaa tttagagtaa
    25261 attttatcta gcatttataa aaaatatttt gaatagcttt tttgttattt agtcataatt
    25321 ttctacttct ttatcaagtt ctttgccaca agaatcacac ttatatatag cattgtatcc
    25381 ataatcatat tcatcaacta ttttcttttg tctaaataca ccaccacact cacataggaa
    25441 accaattttt ataagtgtaa cgataaattg tgattttata aattttatat cattacattt
    25501 atcgttagtg taaaatgtat aaatataaga gttatcgcac tcatctctta attcaaatct
    25561 ttcaatatat gtgttatttt ttaattcctc aaattcttct ttagttaaat atgatttttg
    25621 ttgcaattca tgtaaacgct taatttggtc cattatttat atcctcccaa taattttagt
    25681 attttaagct tagataagga atctaagttt tattaaattt atcccaagga attatttaaa
    25741 tcatatttag ttttattaat tacaacatat ataaattaca cgaaaataaa actaattgta
    25801 aaaataatat cctgtgaaaa cctaaactac taacccctta ctaacccgaa aactaaaaaa
    25861 agaaaatata tccaaatata agtgatatat aaataaagat gttttatata aatttaactt
//
LOCUS       DAMJWP010000035        25745 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.036, whole genome shotgun sequence.
ACCESSION   DAMJWP010000035 DAMJWP010000000
VERSION     DAMJWP010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25745
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.036"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            384..1250
                     /locus_tag="PC700_001232"
     CDS             384..1250
                     /locus_tag="PC700_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2580548.1"
                     /translation="MSEVKNTLQKHPVLRYSTMLFGMLMTSIGINGFLRPAHLLSGGA
                     TGIATSINYLTNINVGLLTFLINIPIFILGFIYLEKEFCISSLVNMIVFSLLLGATQE
                     ISNIIPIHDILLQSVYGGILSGLGVGVVFRTRSSQGGTDIIAAILKIKKNIEMKDTAL
                     AINGLIVLTGSFLFGLDLALYTLIGLFLNAYSMSFIKDAMNYQKSVMVMSNEVDLIAE
                     DIMKSLVRGVTFLDAEGAYTHQKKKIIYTIVSSNEIPKIKDIALKYDKKAFISVNDVT
                     EVKGRGFKAKDL"
     gene            1492..2301
                     /locus_tag="PC700_001233"
     CDS             1492..2301
                     /locus_tag="PC700_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580549.1"
                     /translation="MNLSKEYVNLYDNYLKYINEVNKEIRSIKNMKKRVLKKGDFAPE
                     NEVLELEGKTIRDIDDVDTKKPKNKIYKFSNGDVYVGKFLEGKMEGQGSYTFFVDEGT
                     AMEYIGEFKEDKKNGKGNFVFSNGNEYIGHFEEDMMNGIGNMLYNSKDEYIGTWKNGK
                     KDGKGIYKWSDGCIYAGEFKGGKMEGYGICYNSKGEVLYEGEWKNNLIHGKGTYIWEK
                     GKKYIGEFMHGKKHGQGTFYLNNELVYEGTWKFDKPSIFDRTLDEIFSLRL"
     gene            2444..3508
                     /locus_tag="PC700_001234"
     CDS             2444..3508
                     /locus_tag="PC700_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDF2580550.1"
                     /translation="MKRLIALVLVILIVLFYSPELIEEGYDLKEKFFQSEIWNEFLDK
                     ININNNSKKDKKEQNQNLQSNNTEGENLGESDIKNFEKISLGDNLSYVLSSIGKPGRI
                     DISEYGFDWYVYNQYGKEFAMVGLENDEVVALYSNSINSCENQDIKLNQDRQTVRTKI
                     TPLKYKRKGNTRYIINSENQYDIISKEGKYITIFYDIHEENRVCSYLIIDKSTEDEFK
                     KLYPDDREELKKCFELEVIDLVNSVRNQRGLNSLRYSEQATLSSRKHSEDMRDNNFFD
                     HINKKNETPFDRMKREGIVYTSAGENIAAGQINAIYAHEAWMNSEGHRKNILGNYNNI
                     GVGVIFGGSYKTYYTQNFYK"
     gene            3536..4255
                     /locus_tag="PC700_001235"
     CDS             3536..4255
                     /locus_tag="PC700_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2580551.1"
                     /translation="MTSWKKKTVYKCLIAVVLFCGIVLISNFSKVSALMMDTNGNVLI
                     KHGSREKKLIAITFDDGPHPKETSQVLDVLKKYNVKATFFIAGKHAKWYKEPLVRASK
                     EGHEIGNHTFNHPDISNLSSSQIEEEIVKCEDILKEVTGKKPTLFRPPFGSYREKDLI
                     EIAKKHDYKVVLWTGVDVKDWKNPGANSIADKIINKVKNGDIILLHDYATNDTVEALD
                     MFIPKMIEKGFKFVTVSELIK"
     gene            4279..4863
                     /locus_tag="PC700_001236"
     CDS             4279..4863
                     /locus_tag="PC700_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HDF2580552.1"
                     /translation="MILMIDNYDSFVYNLVQYIEELGEAVVVKRNNEIKISDIEELNP
                     EVIVLSPGPCSPKEAGICIDIVEHFKGKKPILGICLGHQTIGYVFGGDIIKAQQPVHG
                     KVYSINHTNKGVFRGLKNPLNVTRYHSLIIDSNTVPKELEITAITDKGEIMGIRHKKY
                     LIEGVQFHPEAILSEYGHEMLENFITEARERVHM"
     gene            4865..6214
                     /gene="pabB"
                     /locus_tag="PC700_001237"
     CDS             4865..6214
                     /gene="pabB"
                     /locus_tag="PC700_001237"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HDF2580553.1"
                     /translation="MIREINTKLNSFEIFTIFRNEHDSFILDSAMDKEKLGRYSFISS
                     QPFKVLKYKDTDENPLEVLKEELHKYRVVNDTNLPFVGGAVGYLSYDLGNYIENLPRT
                     AVDDIEMPDMYFGFYNHVIVIDHLVQKTYIATPNIDIELEEKIIDDIEQRILKEEKKG
                     IDSICYEEKEVTPIRLKSNFTKEEFKNAVQSVREYIRQGDIYQANLTQRFSGETELTS
                     FELYRDLRRFSPAPFGAFLNFEDTHILSNSPERFIRCVNKRIETRPIKGTRPRGKDKE
                     EDLRLQQELRNSEKDRAELLMIVDLERNDIGRISKTGSVKVPELFVIEPYANVNHLVS
                     TVVGELKDDKDATDVIKATFPGGSITGAPKIRAMEIIDELEPTQRNVYTGSIGYIGFN
                     GDMDFNIAIRTIIKNDKKVYFQVGGGMTWDSDPDEEYQETLDKAKSIMKALRGYYEE"
     gene            6204..6947
                     /locus_tag="PC700_001238"
     CDS             6204..6947
                     /locus_tag="PC700_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HDF2580554.1"
                     /translation="MKNNVGFDSSLSKFGIGLFETIRVKNGIAVDLDIHIERMLSSIN
                     CLDLDINYKKDFLINEIVTYIKKENVINKALRITVFDEGYNISIRDIPYNQETYEKGF
                     KLAISPIVRGNSLIHRHKTTNYFENIYTKNIANKTGYNDGIFLNSEGVILECSMSNIF
                     FIKGERVYTPSDRLPILNGIIKKRIIEICDELHIELIENEINISEISSFDFVFVTNSL
                     MGAIKVTEIDKIKFNKENVVFDKIVKLLE"
     gene            6981..7682
                     /locus_tag="PC700_001239"
     CDS             6981..7682
                     /locus_tag="PC700_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580555.1"
                     /translation="MIGCIYEVYNEEMTKIIENQNTLAVYLVGSSKDVDFTLYDVEIN
                     DIDVFVFVKEGEKQERILFKKKGIEFDVNYFSKDGVKKLLSNREYFFVKEMKDAKVLF
                     DRENISNIIKDISRDIYLEGPSKMSLEEKSFIKQDIGAKISNLKNKEKFDVFEYHFLT
                     NLYLKDIIIGYFNINDKWVPKDKKLLKVLKKENNELFELASKVSENYDYQRLLDVYNY
                     IFKEIETKEVIKLIF"
     gene            7708..8280
                     /gene="folE"
                     /locus_tag="PC700_001240"
     CDS             7708..8280
                     /gene="folE"
                     /locus_tag="PC700_001240"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HDF2580556.1"
                     /translation="MNKVDKEKIQHAVREILEAIGEDPDREGLIETPNRVARMYEEIF
                     SGLSEEPRDHLKVLFADEKHEELVLVKDIPFYSCCEHHLVPFFGKAHIAYLPKGGRLT
                     GLSKLARVIDTLAKRPQLQERITKNAADIIMEELQPYGVLVVVEAEHMCMTMRGVKKP
                     GSKTVTSAVRGIFEKDIASRAEAMSLITMK"
     gene            8346..9137
                     /gene="folP"
                     /locus_tag="PC700_001241"
     CDS             8346..9137
                     /gene="folP"
                     /locus_tag="PC700_001241"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDF2580557.1"
                     /translation="MFDYGKRTYIMGILNVTPDSFSDGGDFNNLDIAIQHAKDMVDQG
                     ADIIDLGGESTRPGHSYVDSDEELRRVIPVIKKLKQELEIPISIDTYKADVAEEALKL
                     GVTMVNDVWGLRKDKNMASVIGKYDAEVCIMHNQDGTNYDKDIMESIKDFFKVSIEMA
                     MSCGVKKEKIVLDPGVGFGKDFEQNIEVLRRLNELKDLGYPILLGTSRKSVIGKVLPV
                     EPKKRLEGTIATTVLGIRDGVDIVRVHDVYENLMAARMTDAIYRK"
     gene            9148..9513
                     /gene="folB"
                     /locus_tag="PC700_001242"
     CDS             9148..9513
                     /gene="folB"
                     /locus_tag="PC700_001242"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HDF2580558.1"
                     /translation="MDKILLSNLGFYGYHGVLKEENFLGQKFFVDMELYIDSREAGLS
                     DDINKSVSYAEVYNVVKDITENKQFNLLEALAENIAEEVLNKFILINGVMVRVRKPEA
                     PVNGIYDYFGVEIRRTRDE"
     gene            9506..10012
                     /gene="folK"
                     /locus_tag="PC700_001243"
     CDS             9506..10012
                     /gene="folK"
                     /locus_tag="PC700_001243"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HDF2580559.1"
                     /translation="MNKAYLGIGTNMGDRFDNLSRACELLKNSDSIYKVKESSLYETK
                     PWGYTEQADFLNMCVEIETEFEPYELLEYCQEIERELHRERIVHWGPRTIDVDILFFN
                     DVASTDERLTIPHPRIQDRAFVLIPLMDLNEELIINEKTVKEHLNLLSAEEREEVKEL
                     VGYERKPI"
     gene            9993..10778
                     /gene="aroF"
                     /locus_tag="PC700_001244"
     CDS             9993..10778
                     /gene="aroF"
                     /locus_tag="PC700_001244"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDF2580560.1"
                     /translation="MKENLFNNNSKIEINTNNKKITVKKDKLIIAGPCAIESYEQLLE
                     TAKFVKSQGANILRGGAYKPRTSPNSFQGLKKEGLKILKAVKDEVGMAVITELMDVRD
                     IDELYSISDIIQIGSRNMQNFTLLSEVGKQNKPVMLKRGIASTITEWIGASEYIAIEG
                     NSNIIMCERGIRTYNDYTRNTLDLAAVPIIQKETGLPVVVDPSHATGVRYLVKPMSLA
                     SFACGADGIMVEVHPDPENALSDGAQSLCFNEFEDLMKSINNY"
     gene            11033..12823
                     /locus_tag="PC700_001245"
     CDS             11033..12823
                     /locus_tag="PC700_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDF2580561.1"
                     /translation="MNDIKDIIEEIKARCDIASIISEYMNIKQSGANYKGLCPFHGEK
                     TPSFYINTSKQIYKCFGCGEGGDVINFVMKMENLDFMDAVKILANKCGIEINTNMNEE
                     TRIKIEKSKKFQDIHTEAARFYLSNLLGSKNLGYEYLRIRGLDDKIIKKFGLGFSLDS
                     WNSLMNTLISKGYKKQDLLDCGLIAKNRDGTNCYDKFRNRVMFPIFDYRGNIIGFGGR
                     VLDDSLPKYLNSPDTLIFNKKQNLYGLNFARKNLESKTIVLVEGYMDLISLYQYGIKN
                     VVATLGTALTEQQGILIKRYADTAIISYDSDEAGIKATLRAIDILTKLGINVKVLDLK
                     DAKDPDEFVRKYGLSDYKKAMDVSTHYIKYKIDHLKKEFNIQKDEERVKFAKEASKII
                     KQLTSPVEIDFYTKYLSNQIDINVESIKREVYGKNYNKPYNNKNQKKIEEKVIEKVEV
                     RQDGKQLVEETLIKIMLEDKKIREIALLKVEESDFLLKESKEILNYMIKNQELDKITI
                     DKLKSLNISEEYLKELNSISLNSINLENTKEIEGIITNIRKNSLEEQINSLLREQQEL
                     ENNNDMKEVDGRVMEIALKIVEINKILKSL"
     gene            12841..14007
                     /gene="rpoD"
                     /locus_tag="PC700_001246"
     CDS             12841..14007
                     /gene="rpoD"
                     /locus_tag="PC700_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDF2580562.1"
                     /translation="MENKSNKKELKKVTAKTLIEKGKKQGSLTLAEIMEAFSETELDK
                     DQVENLYETLGNLGIEITETKNYKADIDFSVADDDLSIGHLDEDAEAISHDDSSAIEI
                     ETVDLSLPKGISIDDPVRMYLKEIGKIPLLKPHEEVEFARRMHEGDEIAKQRLVEANL
                     RLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKFDYTKGYKFSTYATWWIRQAITRA
                     IADQARTIRIPVHMVETINKLIRVSRQLLQELGRDPKPEEIAKEMEMTEDKVREIMKI
                     AQDPVSLETPIGEEEDSHLGDFIPDDDAPAPAEAAAYSLLKEQIEDVLGSLNDREQKV
                     LKLRFGLEDGRARTLEEVGKEFDVTRERIRQIEAKALRKLRHPSRSKKLRDYLD"
     gene            14172..14861
                     /locus_tag="PC700_001247"
     CDS             14172..14861
                     /locus_tag="PC700_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDF2580563.1"
                     /translation="MKLTDRLLKIASLVSDGKKIADIGTDHGYIPVYLLKEGKVPFAV
                     LADVNKGPLDNAHKEVIQNNLLDKVDLRLGSGIEILEIGEVEEVIIAGMGGILISELL
                     EAKKEVAHNVEKLILQPMQAQEELRYYLLNNGYEILEEVLVREDFRIYEIIVAKYTGK
                     NTIIEDEIYYEVGIKLLENKDSLFNDFIEKKIKTYSSIVNKLEGKNGEAIDKKRKESE
                     VAIKKLENLIK"
     gene            14875..15972
                     /locus_tag="PC700_001248"
     CDS             14875..15972
                     /locus_tag="PC700_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDF2580564.1"
                     /translation="MLLKSLTRKIEKKYPLNLAEDWDNVGLIVGDFDMDVKKVLVSLE
                     ANEDVIDEAISKNIDLIVTHHPFIFGKINKINSGDLKGRLIQKLIKNDISLYSMHTNF
                     DIAFDGLNDYFMEIMEFGNSKVLDITKSENLYKLAVYVPHNYSDELRKVLSNSGAGHI
                     GNYSDCTFSIEGEGQFKPLEGSNPFLGSVNDIETVNEVKIETVVPQKLLGGVISSMLD
                     AHPYEEVAYDLYKLENKGEIFGLGRISKLDKSMTLESLSRKIKEKLNMKHIRVVGNLS
                     TDITKVAVVTGAGSEFVKKAKRQGAEVLITGDVKYHEAQDALDIGMCIVDCGHFDTED
                     IFKNVMKRFLDEFSEIEVIKSNVYLNPFSTI"
     gene            16034..16606
                     /locus_tag="PC700_001249"
     CDS             16034..16606
                     /locus_tag="PC700_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDF2580565.1"
                     /translation="MKVAVIFHSVCGSTYLLAREYKEVLEEMNIEVDIFRVSDEVAKT
                     LPQYYLINYKEYKDEFESINVIKSGKEILDYDAIFMGSPTYYGNVSGPMKMFMDSFSD
                     IWVGAPLSGKIFGCFATAGSQHGGGELALQAMNIFAQHMGMTLLSVPCSVRGGYPAYG
                     ILHIAGDNSDIRPNDDAKIGIRDYLKRLNI"
     gene            complement(16666..17133)
                     /locus_tag="PC700_001250"
     CDS             complement(16666..17133)
                     /locus_tag="PC700_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDF2580566.1"
                     /translation="MFKEMRLKKREMTKEDTVEVLKNGEFGTFSTISENGYPYGVAVN
                     YVYFNDSIYFHCARNGHKLDNISKNNKVSFLVVANESVIPDKFSTTYSSAIVFGKACT
                     VENEEKKNALVEIIKKYSKGFFEEGMKYIEKDMNLTTVVKIEIDHISGKASRI"
     gene            complement(17519..17995)
                     /locus_tag="PC700_001251"
     CDS             complement(17519..17995)
                     /locus_tag="PC700_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDF2580567.1"
                     /translation="MLSKAEIKYFNECASEILSSEKVQLMRTFPHHGNVSCLEHSLSV
                     AYYSYLLCKKLHLRVDIQSVIRGALLHDFFLYDWHYKGDRKGLHGFTHPREALKNATL
                     FFQINEKETDIILKHMWPLTVKPPRYKEAFIVCLLDKFCCLVETLKIHSLLSPYHV"
     gene            complement(18115..19206)
                     /locus_tag="PC700_001252"
     CDS             complement(18115..19206)
                     /locus_tag="PC700_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HDF2580568.1"
                     /translation="MSKIECLFTIFPVLILMYATFYFMPYFVGKKHIYGVSIDQEHKN
                     YNYFIKLDKEFKKLLSLGFIIDFILVFILVFTFNKLELSYFIGIIGFLLYESILYIHT
                     HKKAKKLKSEIYSKLGHIDVDSKLIVDMDFINKKNKIIKKFKIIYLIPILFTLGMSIF
                     IAFNYNKLPDSIATHWNINGSPDVFIDKSFLNVFKLIGTDFCLMVLLYITSIGSIKSR
                     IKIDTNRIEESRVENIKYLNKIGYLFLILMIIMTTQFFTTLLSIKTKLSTAMNITTLL
                     VIIYLIATYINSPNLKFNSSYSPEEDEKYWIAGIMYNNPNDPSLMVNKRFGIGWTINF
                     GNPLGKILYIAIALLLIFSLFSLIKSLLL"
     gene            complement(19199..19588)
                     /locus_tag="PC700_001253"
     CDS             complement(19199..19588)
                     /locus_tag="PC700_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2580569.1"
                     /translation="MVLNLDFNSDKSIYVQIKEEITKAIASGELKINESLPSVRSMAE
                     NIGVNLHTVNKSYNELKEEGFLNIDRRKGAIVAQLPMKIDNTTRQKNKLDLELLVAKS
                     YLSGISKEEFLSLSSSLFDKYEVKYYE"
     gene            19780..20226
                     /locus_tag="PC700_001254"
     CDS             19780..20226
                     /locus_tag="PC700_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580570.1"
                     /translation="MLTYLMKRQMHKKDDGMSKPMMITGAVITGVGAYAVYKMVKSHK
                     SNSQMVNTNYYGNSDYDDYKYDEFDYDCDCRDTKQDYEYEELKKKVKEFNSRRINCEN
                     CPHVSQEEMMQYVNSVKDVKEDIDLHEDAKKDNIYKEDEYKKYEEE"
     gene            20490..21215
                     /locus_tag="PC700_001255"
     CDS             20490..21215
                     /locus_tag="PC700_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2580571.1"
                     /translation="MKRKEIVMLKLLIVEDDSTISFGIKYALEQEGFSIDISKDLSSG
                     KEMISSNEYSMILLDVTLPDGTGYELCKYIRGFSQVPIIFLTACDEEVNIVMGLDIGG
                     DDYITKPFRIRELISRIKAVLRRKGNTSEENKKILKFGDLSIYTLEARVYKNDKEIFL
                     TSVEYKLLLILIQNKNTVLSRTQILEKLWDVTYDFVNDNTLTVYIKRLREKIGDDLCE
                     PIYIETVRGIGYKWIGSENSVSI"
     gene            21202..22446
                     /locus_tag="PC700_001256"
     CDS             21202..22446
                     /locus_tag="PC700_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2580572.1"
                     /translation="MFLYNPEVKKFLSKYVTLMFIVIIISIGFSIINVSLTKEMVVRN
                     NQAIIGTLSSKYPNLESQIVDIITQGKSMKNIDYGQKILSKYNYDKSIKINSEPIISK
                     LVLETIKINIILVCIIFILIFVLVVRYFTSIYNDLSDMTKYVYYSSEGKPFDMKNKNQ
                     EGQIGLLKTELLKMTTILNEKVELLKREKIFLNNTISDISHQLKTPMTSLIMLNDLLY
                     NDVPYEVKIDFLNKIKNQLNRMDWLIKSMLKLSKVEAKVINFKKDKVKFSELIHRAML
                     SMKIPMEIKNQKLTIEGNDNVSYIGDIDWSVEALVNIIKNCVEHTPEFGNIIITYKEN
                     PLFSELIIKDEGEGIDKKDIPHVFKRFYRGRSSSKEDSVGIGLAMSKSIIESQNGDIY
                     VNSEKGKGTEFHIIFHKMYDSD"
     gene            22526..23203
                     /locus_tag="PC700_001257"
     CDS             22526..23203
                     /locus_tag="PC700_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2580573.1"
                     /translation="MEILRVENLTKSYGKNETKVDAIKNVSLSVEKGTFIAITGPSGS
                     GKSTLLHLLGGVDRPTSGKVYINDVDIYNLKTKDLAIFRRRNIGLIYQFYNLIPVLNV
                     KENILLPAELDNRDIDREYFDDLMKTLGLIDREKHLPNQLSGGQQQRTSIGRALINRP
                     SIILADEPTGNLDSKNSKEILELLKLSVKKYNQTLIMITHDKNMALQADRIISIEDGI
                     IKSDEVM"
     gene            23204..>25745
                     /locus_tag="PC700_001258"
     CDS             23204..>25745
                     /locus_tag="PC700_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2580574.1"
                     /translation="MNLYTSLTIRYLKQNKRRTIVTIIGIILSTALICGIGNIFESFM
                     DYQMRETIKNDGSFHVTFYDVNRKNVEYVTKSAEIEKHAFTKQLGYSKLENSENAILS
                     IKQYDQNAINGYKVSIKEGRFPAKEGEIVLSESIINLIDDKLKIGDKITLKVGDMFDS
                     SKKKIDSMTFHEGDYIANEKDRTFKVVGIIKKPGFERYNGIATAIAYFNPMNNYNDTI
                     NVSVAVRNPKDVYKISNKISKNIYSNKDKEAVSDMIKYNEHLLRLQGASKYSNINSSI
                     KSIIIAVTILVIICTIATVYNSFSISINERKKQFGILNSIGATSSQIKRLVFIEGIII
                     SLIGIPIGLISGTVAIDLLFKIINKYFTESVVTKMSLQIVYNPIIIIVSIIIVLLTIF
                     ISILLPAISASNISPLNVIKNTGEYKVGKIKSSRLIKMIFKTEGVLAYKNIRRNRKKF
                     IITLFSLMVSIIIFISFSGFTLLLLRGEEIRNSQRKYDLYLTAKNTARSVEDTISELE
                     DIDGIKNFELATGQYVSIRVSENKINKSKEDLIRKYYQKYKIGESYEYDFSNNELIFP
                     GDFAVKNKINNLVQGSFNKERAIEENGVILVRKSAIEENGKKGVVELTNYKVGDTVNC
                     EYLDENALSKKVKVKILAITDEERLGFGYQNMGLQFITYDEVAKNLNLKLSKSLIFID
                     SGGSIKTKKEIEALANKNDFNFYDEISSSESEKQDLKVIKIFVYGFIVVISLVSVTNI
                     LNTVSTSINLRKRELAVIQSIGVTPKGFRKMIYLESFIYGILSLLFGIPISIGIILIM
                     NKLISGVIEFSPIIPWTAIVICIVSVFIITFIAGYIPMSKLNKENIIDN"
BASE COUNT        10540 a         2569 c         4550 g         8086 t
ORIGIN      
        1 catttaggaa ttaattataa agagataatt actaaacttg aatttgctaa aataaaataa
       61 attactgcga aaatagatat ttagaaaaaa atactataaa tttactatat tgcaagttaa
      121 ataataagca gtatcatatt atgctagaaa aagtttcatt taaggcataa atgagatgat
      181 ttgaacgttt ttaaagatta gaatataaaa gattgcacta gacatatatg aaaaagataa
      241 tataatagat gatgtaatag atgacagttc aagaaattat aaaaaaaatt tatttgaaat
      301 attatctatt acatcaggaa taatatatgt aagagtaagc ttttaaaatt ctaaagattt
      361 agaaagattg aagggagaaa gaaatgagtg aagttaagaa taccctacaa aaacatccag
      421 ttttaagata ttcaacaatg ctatttggga tgttgatgac atctatagga ataaatgggt
      481 tcttaagacc agcacatctg cttagtggag gtgctacagg tatagcaaca tctataaact
      541 atttgacaaa tataaatgta ggtttattaa catttttaat caatattcca atatttatct
      601 tagggttcat ataccttgaa aaagagtttt gtatttcaag tttggtaaat atgattgtat
      661 tttcattatt attaggagca actcaggaaa taagtaatat tatacctata catgatatat
      721 tgcttcaaag tgtatatggt ggtatattat ctggtttagg agtcggagtt gtatttagaa
      781 ctaggtcttc tcaaggtggt acagatataa tagctgcaat actaaaaatt aagaagaata
      841 tagaaatgaa agacactgct ttagcaataa atggcttgat agtacttaca ggaagttttt
      901 tatttgggtt ggatttagca ttatacactc ttataggtct atttctaaat gcgtattcaa
      961 tgagttttat taaagatgca atgaattacc aaaaatctgt tatggtaatg tcaaatgaag
     1021 tcgatttaat agcagaagat ataatgaaga gtttagttag aggtgtaact tttttagatg
     1081 cagaaggtgc atacactcat caaaaaaaga agattatata tacaatagtt tcatcaaatg
     1141 agataccaaa gataaaagat atagctttga agtatgataa aaaggccttt atatctgtaa
     1201 atgatgttac agaagtaaaa ggaagagggt ttaaagcaaa agatttatag aaaattttac
     1261 aagctgggag tttataatgt aaatttgtat attatatctc tcagcttatt taaatttagt
     1321 atatgaaaat aaaggtttta tcaatgattt gtgtatcact ttaaaagata tcattttaaa
     1381 attaaaattg aaaaaattac tcaatatgta gaaaacataa ccactattag gatataataa
     1441 atttaataga tttagtaaaa tatctaggaa taacgttgga ggaatagcat tatgaattta
     1501 tctaaagaat atgttaattt gtatgataat tatttgaaat atataaatga agtaaacaaa
     1561 gagattagaa gtataaagaa catgaagaaa cgtgttttaa aaaagggcga ttttgctcca
     1621 gaaaatgaag tattagaatt ggaaggaaaa acaatacgcg atatagatga tgtcgataca
     1681 aaaaaaccaa aaaataaaat atataaattt tcaaatgggg atgtgtatgt aggaaaattt
     1741 ttagaaggaa agatggaagg gcaaggatca tatacttttt ttgttgatga aggaacggct
     1801 atggagtaca taggagagtt taaagaagac aaaaaaaacg gaaaaggaaa ctttgtattt
     1861 tcaaatggaa atgaatatat aggccatttt gaggaagata tgatgaacgg tattggaaat
     1921 atgttatata atagcaaaga tgaatatatt ggaacttgga aaaatggaaa aaaagatgga
     1981 aaaggtattt ataagtggag tgatggatgt atatatgcag gtgaatttaa aggtggaaag
     2041 atggaagggt atggtatttg ctataatagt aaaggggaag tactttatga aggagaatgg
     2101 aagaataatt tgattcatgg taaaggaact tatatatggg aaaaaggtaa aaagtatata
     2161 ggtgaattta tgcatggtaa aaaacatgga caaggaactt tttatctaaa taatgaattg
     2221 gtttatgaag ggacttggaa atttgataag ccatccatct tcgacagaac attagatgaa
     2281 atattttcat taagactata gctttatcta ttaatagata aagctttttt ctaatactta
     2341 ctgtaaataa gtatgaatga tattgattag tatacactat caatattaac tcaataaatg
     2401 tgatattaaa aatttttgat tgattttaag aaggagggat atattgaaga gattaattgc
     2461 tttagtgtta gtaatactta ttgttttatt ttatagccct gaattgatag aagaaggata
     2521 tgatttaaaa gaaaaatttt ttcaaagtga aatatggaat gaatttttag acaaaataaa
     2581 tataaataat aattctaaga aagataaaaa agaacaaaat caaaatttac aatcaaataa
     2641 tactgaaggg gagaacttag gagagtcaga tataaagaat tttgaaaaaa taagcttagg
     2701 agataattta agctatgttt tatcaagtat tggtaaacct ggtagaattg atattagtga
     2761 atatggtttt gattggtatg tatataatca atatggaaaa gaatttgcaa tggtcggact
     2821 tgaaaatgat gaagtagtag cactttatag taattctatt aattcatgtg aaaatcaaga
     2881 tataaaacta aatcaagata gacaaacagt gagaactaag attacaccat taaaatataa
     2941 gagaaaagga aataccagat atataataaa ctcagaaaat caatatgata taataagtaa
     3001 agaaggtaag tatattacaa ttttctacga tattcatgaa gaaaatagag tttgctcata
     3061 tttaataatt gataaaagta ctgaagatga gtttaaaaaa ttatatccag atgatagaga
     3121 agaacttaaa aaatgttttg aacttgaagt tatagacctt gtaaatagtg ttagaaatca
     3181 aagaggatta aactcattga gatacagtga acaggcaact ttgagttcaa gaaaacatag
     3241 tgaagatatg agagataata atttttttga ccatataaat aagaagaatg aaactccatt
     3301 tgacagaatg aaaagagaag gtatagtata tacttcagct ggagaaaaca tagcagcagg
     3361 acagattaat gcaatatatg cgcatgaagc ttggatgaac tctgaaggac atagaaaaaa
     3421 tattttgggg aactataata atattggagt tggagtaata tttggaggaa gctataaaac
     3481 atattacact caaaattttt ataaataact aaaatatttg aaaggaagtt aaaaaatgac
     3541 atcatggaaa aaaaagacag tatataagtg cttaatagca gtagtcttgt tttgtggaat
     3601 agtgttaata tctaatttta gtaaagtttc tgctttgatg atggatacta atggaaatgt
     3661 tttgattaaa catggttcaa gggaaaaaaa gctaatagca attacctttg atgatggtcc
     3721 tcatcctaaa gaaactagtc aagttttaga tgtattaaag aaatacaatg taaaagctac
     3781 tttttttata gctggaaaac atgcaaaatg gtataaagaa ccattagtaa gagcaagtaa
     3841 agaaggtcac gagataggaa atcatacttt caatcatcct gatataagta atttaagttc
     3901 tagtcagata gaagaggaga ttgttaagtg tgaagacatt ttgaaagaag taactggaaa
     3961 aaaacctaca ttatttagac caccatttgg aagttataga gaaaaagact taatagaaat
     4021 tgcaaaaaag catgactata aagttgtttt atggacaggt gtagatgtga aagattggaa
     4081 aaatccagga gctaatagca ttgcagataa aataattaat aaagtaaaaa atggagatat
     4141 aatacttttg catgattatg caactaatga tacagttgaa gctttagata tgtttattcc
     4201 aaaaatgatt gaaaaaggtt ttaaatttgt aacagtttct gagttgataa aataattttt
     4261 aggggggaga tttacagtat gattcttatg atagacaatt atgattcttt tgtatacaac
     4321 ctagtccaat atatagaaga attaggggaa gcagttgtag taaaaagaaa taatgaaatc
     4381 aaaataagtg acattgaaga actaaatcca gaggtaattg tgttatctcc aggaccgtgt
     4441 tctccaaaag aagctggtat ttgcatagat attgttgagc attttaaggg gaaaaaacct
     4501 atactaggaa tttgtttagg tcaccaaact attggatatg tttttggagg agatattata
     4561 aaagcacaac agcctgtgca tggaaaagta tatagtatca accatactaa taaaggagtt
     4621 tttaggggac ttaaaaatcc tttaaatgtc actaggtatc attcactaat aattgattcc
     4681 aatacagttc caaaagaatt agaaattaca gcaattactg ataaagggga aataatgggg
     4741 attaggcata agaaatattt aatagaagga gtacagtttc atccagaagc tattttgtca
     4801 gagtatggtc atgaaatgct tgaaaatttt ataacagaag ctagagaaag ggtacatatg
     4861 taatatgata agagagataa atactaaatt aaattctttt gagatattta ctatttttag
     4921 aaatgaacat gacagtttta tattagacag tgctatggat aaagaaaaat tgggaagata
     4981 ttcttttata agtagccaac catttaaggt attaaagtat aaggatactg atgaaaatcc
     5041 tttagaagtt ttaaaagaag aactacataa gtatagggtt gtaaatgata ctaatcttcc
     5101 atttgtagga ggtgctgtgg gatatttatc ttatgattta ggtaattaca tagaaaattt
     5161 acctagaact gctgtagatg atattgagat gcctgatatg tattttggat tttataatca
     5221 tgtaatagtt atagaccatc ttgtgcaaaa aacttatata gctactccaa acatagatat
     5281 cgaattagaa gaaaagataa tagatgatat agaacaaaga atattaaaag aagagaaaaa
     5341 aggtatagat agcatatgtt atgaagaaaa agaagtaaca cctataagac ttaaatctaa
     5401 ttttacaaag gaagaattta aaaatgcagt tcaaagtgtt agagagtaca taagacaagg
     5461 agatatatat caagctaatt taacacaaag gttcagtgga gaaacagaac ttacaagctt
     5521 tgaattgtat agggacttaa gaagatttag cccagcacca tttggggcat tcttaaactt
     5581 tgaagataca catatattat caaattctcc agaaagattt ataagatgtg ttaataagag
     5641 aatagaaact agaccaataa aaggtactcg tccaagaggg aaagataaag aagaagattt
     5701 aagacttcaa caagaactta gaaatagtga aaaagataga gctgaactac ttatgatagt
     5761 agatttagaa agaaatgata ttggcagaat atctaaaact ggaagtgtta aagttccaga
     5821 gctatttgta attgaaccat atgcaaatgt aaatcactta gtttccacag ttgtaggtga
     5881 gttaaaagat gataaagatg ctactgatgt aataaaagca acttttccag gggggtctat
     5941 aacaggagca cctaagatta gagcaatgga aattatagac gaattagagc caacgcaaag
     6001 aaatgtatat actggttcaa taggttatat aggttttaat ggtgatatgg attttaatat
     6061 agcaattaga acaataatca aaaatgacaa aaaagtctat tttcaagttg gaggaggaat
     6121 gacttgggat tctgacccag atgaagaata tcaagaaaca cttgataaag ctaaatccat
     6181 aatgaaggct ttgaggggat attatgaaga ataatgtagg ttttgatagt agtttaagta
     6241 aatttggaat tggtttgttt gaaactatta gagttaaaaa tggaatagca gtagatttag
     6301 atatccatat agaaagaatg ctaagttcaa taaattgttt ggatttagat ataaactata
     6361 aaaaagattt cttgataaat gaaattgtta catatataaa aaaagaaaat gtcattaata
     6421 aagcacttag aattactgtt tttgatgaag gttataatat atctattagg gatattccat
     6481 ataatcaaga gacgtatgag aaggggttta aattagctat atcgccaata gttaggggga
     6541 atagtttaat tcatagacat aagactacta attattttga aaacatatat accaaaaata
     6601 tcgcaaataa gactggttat aatgatggta tatttttaaa ttcagaaggt gtaatattag
     6661 aatgttctat gagcaatatt ttctttataa aaggagaaag agtgtacact cctagtgata
     6721 gattaccgat attaaatgga ataataaaaa aaaggattat agagatttgt gacgaattac
     6781 atatagagtt gatagaaaat gaaattaata tatcagaaat tagtagtttt gattttgttt
     6841 ttgtcacaaa tagtcttatg ggcgctataa aggttacaga aattgacaaa ataaaattta
     6901 ataaagaaaa tgttgttttt gataaaatag tgaagttatt agaataaatt aaaatattaa
     6961 caatttgttg gagacaaaag atgataggtt gtatttatga agtatacaat gaagagatga
     7021 ctaaaataat agaaaaccaa aacactttgg ctgtatattt agtaggttct agtaaagacg
     7081 tagattttac tctttatgat gtagagataa acgatattga tgtgttcgtt tttgtaaaag
     7141 aaggggaaaa acaagagaga atactgttta aaaaaaaggg aatagaattt gatgtaaatt
     7201 acttttcaaa agatggtgta aaaaaattat tatcaaatag ggaatatttc tttgtgaaag
     7261 aaatgaaaga tgctaaagta ctgtttgata gggagaatat atcaaatatt ataaaagata
     7321 taagtagaga tatttactta gaaggaccca gtaaaatgtc attagaggaa aagtctttta
     7381 taaagcaaga tattggagca aaaatatcga atttaaaaaa taaagaaaaa tttgatgttt
     7441 ttgaatatca ttttttgacg aatttatatt taaaagatat aataattggg tactttaata
     7501 taaatgataa atgggtacca aaagacaaaa aattgctaaa agttttaaaa aaagaaaata
     7561 atgagctttt tgaactagcg tcaaaagtaa gtgaaaatta tgactatcaa agacttttag
     7621 atgtttataa ttatattttt aaagaaattg agacaaaaga ggttataaaa ctaatcttct
     7681 agtcacaaat caatgggggg agtttaaatg aataaagttg ataaagaaaa gatacaacat
     7741 gcagtaagag aaatactaga ggcaattgga gaagacccag atagagaagg tctaatcgag
     7801 actccaaata gagttgctag gatgtatgaa gagatattct ctggtcttag tgaagaacca
     7861 agagaccatt taaaagttct ttttgcagat gaaaaacatg aagaattagt attggtaaaa
     7921 gatattccat tttattcatg ttgtgagcat catctagttc ctttctttgg aaaagctcat
     7981 attgcttatt taccaaaagg aggaagactt acagggcttt ctaaacttgc tagagtaata
     8041 gatacattgg caaaaagacc acaattacaa gaaagaatca ctaaaaatgc tgcagatata
     8101 ataatggaag aattacaacc ttatggtgtc ttagtagtag tagaagctga acatatgtgt
     8161 atgacaatga gaggtgtaaa aaaaccaggt tcaaaaactg ttacatcagc agtgagagga
     8221 atatttgaaa aggacattgc gtctagagcc gaggctatga gtttgattac tatgaagtag
     8281 caatttatta tagatataat catttattga gatataaaat ttaatttaag ggagtgtcat
     8341 taattgtgtt tgattatggt aaaagaactt atataatggg aatattaaat gtaactccag
     8401 atagtttttc tgatggagga gattttaata acttggacat agcaatacaa catgcaaaag
     8461 atatggttga ccaaggtgca gatataatag acttaggagg agagtctaca cgcccaggtc
     8521 atagctatgt agactcagac gaagagctaa gaagagtaat ccctgtaata aaaaaactta
     8581 aacaggaatt ggagatacca atatcaatag acacttataa ggcagatgtt gcagaagaag
     8641 cattaaaatt aggtgtcact atggtaaatg atgtttgggg tcttagaaaa gataaaaata
     8701 tggcttctgt aataggaaaa tatgatgcag aagtgtgtat aatgcataat caagatggga
     8761 caaactatga caaagatata atggagtcaa taaaagactt ttttaaggta agtatagaaa
     8821 tggcaatgag ttgtggtgta aaaaaagaaa aaatagtact tgacccaggc gtaggatttg
     8881 gcaaggattt tgaacaaaat attgaagtat tgagaagatt aaatgaatta aaagatttag
     8941 ggtatcctat tttgcttggg acatccagaa aatcagtaat aggaaaagta cttccagttg
     9001 agcctaaaaa aagattagaa ggtacaatag ctactactgt tcttggtata agagatggcg
     9061 tagatatagt tagagtacat gatgtttatg aaaatttaat ggcagctaga atgactgatg
     9121 caatatatag aaagtaggta taatataatg gataaaatat tacttagtaa tctaggattc
     9181 tatggatatc atggtgttct taaagaagaa aattttctag gtcagaaatt ctttgtcgac
     9241 atggaacttt atattgattc aagagaagca ggtcttagtg acgatataaa taaatctgta
     9301 agctatgccg aggtttacaa tgttgttaaa gatattactg aaaataaaca atttaatctt
     9361 ttggaggctt tagcggaaaa tattgctgaa gaagtcttga ataaatttat acttataaat
     9421 ggagttatgg tcagagttag aaagcctgag gctcctgtaa atggtattta tgactatttt
     9481 ggtgttgaga taaggagaac aagagatgaa taaggcttat ttgggaatag gtactaacat
     9541 gggtgataga tttgataatt tatcaagagc atgtgaactt ttgaaaaata gtgattctat
     9601 atataaagta aaggaatcaa gtttatatga aacaaaacca tggggatata cagaacaagc
     9661 agattttcta aatatgtgtg tagaaataga aacagaattt gaaccttatg agttgttaga
     9721 atattgtcaa gaaatagaga gagaactaca cagagagaga atagttcatt ggggaccaag
     9781 aactatagat gtagacatac tattttttaa tgatgtagct tccactgatg aaagactaac
     9841 aatacctcat ccgaggattc aagatagagc tttcgttttg ataccactta tggatttaaa
     9901 tgaagaactt ataataaatg aaaaaacagt aaaagaacat ttaaacctcc tatctgctga
     9961 agaaagagaa gaagtaaagg agcttgtagg ttatgaaaga aaacctattt aataataatt
    10021 caaagataga gataaataca aataacaaaa agataactgt aaaaaaagat aaacttataa
    10081 tagctggacc atgtgcaata gagagttatg agcaactttt ggagactgct aaatttgtaa
    10141 aaagtcaggg agcaaatatt ttaagaggag gagcgtacaa gccaagaaca tcgccaaatt
    10201 catttcaagg attaaaaaaa gaaggtctta aaatattaaa agcagttaaa gacgaagttg
    10261 gtatggctgt tattacagaa cttatggatg taagagatat cgacgaactg tatagtataa
    10321 gtgatataat tcaaattggt tcaagaaata tgcaaaactt tactctttta agtgaagtgg
    10381 gaaaacaaaa taaacctgtt atgttaaaaa gaggaatagc atccacaatt actgaatgga
    10441 taggtgcttc tgaatatata gcaatagaag gaaatagtaa cattataatg tgtgagagag
    10501 gtataagaac gtacaatgat tatactagaa atacattaga tttagctgcg gtacccatta
    10561 ttcaaaagga aacaggtctt cctgttgtgg tagacccaag tcatgccact ggagttagat
    10621 atttggtaaa acctatgtca ctggcatctt ttgcatgtgg agcagatgga ataatggtag
    10681 aagtacatcc tgacccagaa aatgctttgt cagatggtgc tcaatctctt tgttttaatg
    10741 aatttgaaga tttaatgaaa tcaattaata attattaaaa gaggcaagag cctctttttt
    10801 attcgtaatc attgctaagg taaatttata agttataatt tgatagttag attatttaat
    10861 ataaataaag atttaatatt gatagaatta atataaaaaa taaaaatttt ttgattaata
    10921 agaaggatat caataaaaaa agtcgaattg tgtatattga gtgattgaca aatttagaat
    10981 atagtgtaga atagaaatgt taacacaaaa aattacacca gaaggtgata atatgaatga
    11041 tataaaagac attattgaag aaataaaagc tagatgcgat atagcaagta taatatctga
    11101 atatatgaat ataaaacaat ctggagctaa ttacaaaggg ttatgtccat ttcatggtga
    11161 aaagacacct tctttttata taaatacatc aaaacagatt tataagtgtt ttggttgtgg
    11221 tgaaggtgga gacgtaataa attttgtcat gaaaatggaa aatttggatt ttatggatgc
    11281 tgtaaaaatt ttagcaaata aatgcggtat agaaattaac actaacatga atgaagaaac
    11341 taggattaaa atagaaaaat ctaagaaatt tcaagatatc catacggaag cagctagatt
    11401 ttatctttca aacttattgg gaagcaaaaa tcttgggtat gaatatttaa gaataagagg
    11461 actagatgat aaaattatta agaaatttgg tttaggattt tctttggatt catggaattc
    11521 acttatgaat actctaataa gtaaaggata caaaaaacaa gacttattgg attgcggtct
    11581 aattgcaaaa aatagagatg gaactaattg ctatgataaa tttagaaata gagttatgtt
    11641 tcctatattt gactatagag gaaacataat aggatttggg ggaagagtat tagatgattc
    11701 tttgccaaaa tacttaaatt ctccagacac attaattttt aataaaaaac agaacttata
    11761 cggattaaat tttgctagaa aaaatttaga aagtaaaact atagtcttag ttgaaggata
    11821 tatggattta atttcgcttt atcagtatgg tataaaaaat gtagtagcaa cccttggaac
    11881 agccttgact gaacaacaag gcattttaat aaagagatat gcggacactg ctattatatc
    11941 ttatgattct gatgaagcag gaataaaagc gactttaaga gctatagata tacttactaa
    12001 attaggtatc aatgtaaaag tgttagactt aaaagatgct aaagacccag atgaatttgt
    12061 aagaaaatac ggacttagtg attataaaaa agctatggat gtttctactc actacataaa
    12121 atataaaata gatcatctta aaaaagaatt taacattcaa aaagatgaag aacgagtgaa
    12181 gttcgcaaaa gaagcttcta aaattataaa acaattgaca agtcctgttg aaattgattt
    12241 ttatacaaag tatttaagca atcaaataga tattaatgtc gagtctataa agagagaagt
    12301 ttatggcaaa aattacaata agccatataa taacaaaaat caaaagaaaa tagaagaaaa
    12361 agtaattgaa aaagtcgaag tcagacaaga cggaaaacaa ttagttgaag aaactctaat
    12421 aaaaataatg ttagaagaca aaaaaattag agaaatagcg ttacttaaag tagaagaaag
    12481 tgatttttta ttgaaagaaa gtaaagaaat tttaaattac atgattaaaa atcaagaatt
    12541 ggacaaaata actattgaca aattgaaaag tttaaacata tccgaagaat acttaaaaga
    12601 attaaattca atttctttga atagtataaa cttagagaac acaaaagaaa tagaaggaat
    12661 aataacaaat atcagaaaaa actcattaga agagcaaatt aatagcttgt tgagagaaca
    12721 acaagaatta gaaaataata atgatatgaa agaggtagat ggtagagtta tggaaattgc
    12781 tttaaaaata gttgagataa ataaaatttt aaagagcttg tagagaaagg aggtttgagt
    12841 gtggaaaata aatcgaataa aaaagagtta aaaaaggtta ctgctaagac attaatagaa
    12901 aaaggaaaaa agcaaggttc tctgacactt gcagagataa tggaagcttt ttcggagact
    12961 gaacttgata aggatcaagt agaaaatctt tatgagactt taggaaattt gggaatagaa
    13021 ataacagaaa caaaaaatta taaagctgat atagattttt cggttgctga tgacgattta
    13081 agtataggcc acttagatga agatgcagag gcaatttcac atgatgactc ttctgcaata
    13141 gaaatagaaa ctgtggattt atctttacca aaagggataa gtatagatga ccctgtaaga
    13201 atgtacttaa aagaaatagg aaaaattcct ctacttaaac cacatgaaga agtagaattt
    13261 gctagaagga tgcacgaagg tgatgagata gcaaaacaaa ggttagttga agcaaactta
    13321 agactagttg taagtatagc aaaaagatat gtaggaagag gtatgctttt cttggattta
    13381 atacaagagg gaaatttagg tcttataaaa gcagtcgaaa aatttgacta tacaaaagga
    13441 tataagttta gtacttatgc aacatggtgg ataagacaag ctataacacg tgctattgca
    13501 gaccaagcta gaactataag aataccagtt catatggtag agactataaa taagctaata
    13561 agagtatcaa gacaactact tcaagaactt ggaagagatc caaaaccaga agaaattgca
    13621 aaagaaatgg aaatgacaga agataaagta agagaaataa tgaaaatagc tcaagaccct
    13681 gtgtctttag aaacaccaat aggagaagaa gaagatagcc atttaggtga ttttattcca
    13741 gatgatgatg ctccagcccc agcagaggca gcagcatatt ctttattaaa agaacaaata
    13801 gaagatgtac ttggttcgtt aaatgataga gaacaaaaag tattaaagct tagatttggt
    13861 cttgaggatg gtagagccag aactcttgag gaagttggaa aagagtttga tgtaactaga
    13921 gagagaataa gacagataga agcaaaagca ctaagaaaac taagacatcc aagtagatca
    13981 aaaaaactta gagattattt agactaaaaa gatacatcat tgatgttttt gtgataaatg
    14041 ccatgagata agtgatatct catggctatt ttgtagtaaa gatttaattt tatttttgta
    14101 gttatttcag tctaatatct ataacttgga ttaaatccta taaataaagg gaattgaaag
    14161 gagaattata tttgaaatta acagatagat tattgaaaat agcatcatta gtgtcagatg
    14221 gtaaaaaaat agctgatata gggacggacc acggatacat accagtatat ttgttaaaag
    14281 agggaaaagt accatttgca gtgctagcag atgttaataa aggacctctt gacaatgcac
    14341 ataaagaagt catacagaac aatcttttag ataaagttga tttacgattg ggttctggta
    14401 tagagatact tgaaataggg gaagtagaag aagtgataat tgctggtatg ggtggaatct
    14461 tgataagtga gcttctcgag gctaaaaaag aagtagcaca taatgtagaa aaattaatat
    14521 tacagccaat gcaagcacaa gaagaactta gatattacct tttaaacaac ggatatgaaa
    14581 ttttagaaga agttttagtg agagaagatt ttagaatata tgagataatt gttgccaaat
    14641 atacaggtaa aaatactata atagaagatg agatatatta tgaggttgga ataaagcttt
    14701 tagaaaacaa agattctctt tttaatgatt ttatagaaaa aaagataaag acatatagtt
    14761 ctatagttaa taaattagaa gggaaaaatg gggaagctat agataagaag agaaaggaaa
    14821 gcgaagttgc aataaaaaaa cttgaaaatt taataaaata aataggaggt atatatgcta
    14881 ttaaagagtc taactagaaa aattgaaaaa aagtatcctc taaacttagc agaggattgg
    14941 gataatgtag gtcttatagt tggagatttt gatatggatg taaaaaaggt attggtatct
    15001 ttagaagcta atgaagatgt tattgatgaa gctatatcca aaaatataga tttaattgtg
    15061 acacatcatc cttttatatt tgggaaaatt aataaaatta atagtggaga tttaaaaggc
    15121 agacttatcc aaaaacttat taaaaatgat atatcacttt attctatgca tacgaatttt
    15181 gatatagcat ttgatggtct aaatgactat tttatggaaa ttatggaatt tggtaattca
    15241 aaggttttag atataactaa aagcgaaaat ttgtataaat tagcagtata cgttccacat
    15301 aactattcag atgaacttag aaaggtttta agtaattcag gagcaggtca tatagggaat
    15361 tatagtgatt gtacgttttc aatagaaggt gaaggacaat ttaagccatt agaaggctct
    15421 aatccgtttc ttggaagtgt aaatgatata gaaactgtaa atgaagttaa aattgaaact
    15481 gtagtaccac agaagctttt aggtggggta ataagttcta tgttagatgc acatccttat
    15541 gaagaagttg catatgattt gtacaagtta gaaaataaag gtgagatatt tggactaggt
    15601 agaataagta aacttgataa aagtatgaca ttagaaagtc tttcaaggaa aataaaagag
    15661 aaattaaata tgaaacacat aagagtagtt ggaaacctta gtacagacat aacgaaagta
    15721 gccgttgtaa caggagctgg ttcagaattt gtaaaaaaag ctaagagaca aggtgcagaa
    15781 gtactaatta cgggtgatgt aaagtatcat gaggcacaag atgcacttga tattggaatg
    15841 tgtatagtgg attgtggtca ttttgataca gaagatatat tcaaaaatgt tatgaagaga
    15901 tttttagatg aatttagtga aattgaagta ataaagagta atgtatattt gaatccattt
    15961 agtactattt agaagtgttt gattaaaaaa tactatataa aaaataaatt tgagtttaat
    16021 tagggggata atgatgaaag tagcagtaat ttttcatagt gtttgtggga gtacatattt
    16081 attagctaga gaatataaag aagtgcttga agaaatgaat atcgaggtag atatatttag
    16141 agtaagtgac gaagttgcaa aaacacttcc tcaatattat ttgataaatt acaaagaata
    16201 caaagacgaa tttgaaagta taaatgtaat taaatctgga aaagaaatat tagattatga
    16261 tgctatattt atgggttcac caacatatta tggaaatgta tctggaccaa tgaagatgtt
    16321 tatggatagt ttttctgata tttgggtagg tgctcctttg agtggtaaaa tatttggttg
    16381 ttttgctaca gcaggttcac aacatggagg aggagaatta gctttgcaag ctatgaatat
    16441 atttgctcaa catatgggaa tgactctttt gtcagttcca tgttcagtaa gaggaggtta
    16501 tccagcgtat ggaatacttc atatagcagg agataattct gatataagac caaatgatga
    16561 tgccaagata ggtataagag attacctaaa aagacttaac atataagaat atcatataaa
    16621 aatacctaaa agtagctgta tatactttta ggtattttta cttaattata tacgtgaagc
    16681 tttaccagaa atatggtcaa tttctatttt aacaacagtt gttaagttca tatccttctc
    16741 tatgtacttc atgccttctt caaagaatcc tttagaatat ttttttatta tttctacaag
    16801 agcatttttc ttttcttcat tttctacagt gcaagctttt ccaaaaacta tagcactaga
    16861 ataagtagta ctaaatttat ctggaataac actttcatta gcaactacta aaaaagaaac
    16921 tttgttgttt ttcgatatat tatctaactt atgtccattt cttgcacaat gaaagtaaat
    16981 agaatcatta aagtatacat aatttacagc aactccataa ggataaccat tttcagatat
    17041 agtggaaaaa gtaccaaatt caccattttt taatacttca acagtatctt cttttgtcat
    17101 ttctcttttt ttcaatctca tttctttaaa cataatatca tcctttctaa ttttgatgct
    17161 taaattaaat tttagcacat ttataaagat tatgctatgt tttttacaaa ttttaagaaa
    17221 aaaatatagt ttaagataaa aggtagtgaa agagaagcta tttggaggtt tatttatgat
    17281 attattgtta gagtagtaca aacaatacta ataaatttaa tagctggttt cttgttattt
    17341 aaggtagtaa gcatgtctat aagaagggga agttatcgag ggtagaagtt gttatttata
    17401 acgatattat aaagaaaata ttattatata aaataataaa tagtaaagag gtatcaagtt
    17461 gaatataata cttaatacct cttcaaaatt aaattataaa agtgttgtgg gaattgtcct
    17521 atacatggta aggagataat aaagaatgta tctttaaagt ctcaactaaa cagcaaaatt
    17581 tatccaacaa acatacaata aatgcttctt tgtatctagg tggcttaaca gtaagtggcc
    17641 acatgtgttt taaaattatg tccgtttctt tttcatttat ttgaaaaaat aaagttgcat
    17701 tttttaatgc ttctctaggg tgagtaaatc catgtaatcc ttttctatca cctttgtaat
    17761 gccaatcata aagaaaaaag tcgtgtagta aagctcctct aataacactt tgaatatcaa
    17821 cacgtaagtg caatttttta cataatagat aactataata tgctactgac aaactatgtt
    17881 caagacatga aacattgcca tgatgaggaa atgttctcat aagttgaact ttttcagaag
    17941 aaagtatctc agaagcacat tcattaaaat atttgatttc agcttttgac aacattatga
    18001 aaatctccat tcttagtaat atataaatac tatacttcaa cgataatatg aaatatttct
    18061 tatcctacaa aatataatac ccaataatac aagtaagata ccttttatgg tattttaaag
    18121 taagagagat tttattaggc taaacaatga aaaaattaaa agtaaagcta tagcaatata
    18181 tagtattttt ccaagaggat tgccaaaatt tatagtccat ccaataccaa accttttatt
    18241 aaccattaat gatgggtcat ttggattatt atacatgatg cctgctatcc aatacttctc
    18301 atcttcttct ggagaataag atgagttgaa ttttaaattt ggggaattaa tatatgttgc
    18361 tattaaataa attataacta aaagcgttgt aatattcata gcagttgata gttttgtttt
    18421 tattgataaa agagtagtaa aaaactgtgt tgtcattata atcatcaata ttaaaaacaa
    18481 ataaccaatt ttatttaaat atttaatgtt ttcaactcta ctttcttcaa ttctatttgt
    18541 atctatctta atccttgact ttatggatcc aatggaagtt atatataata aaaccattaa
    18601 acaaaagtca gtaccgatta atttaaatac attaagaaat gatttatcta taaaaacatc
    18661 aggagaccca tttatgttcc aatgagtggc aattgagtct ggcaatttat tataattaaa
    18721 tgctataaaa atactcatcc ccaaagtaaa tagtatcggt attaaataga ttattttaaa
    18781 tttttttatt attttatttt tcttatttat aaaatccata tctactatta attttgaatc
    18841 tacatctata tgacctaatt tagaataaat ttctgattta agcttcttag cttttttatg
    18901 tgtatgaata tatagtatac tctcatataa taaaaaacct ataataccaa taaaatatga
    18961 aagttccaat ttattaaatg taaaaaccag aataaaaact aatataaagt ctataataaa
    19021 acctaaagat aatagttttt taaattcttt atctaatttt ataaagtaat tataattttt
    19081 atgctcttgg tctatagaaa ctccatagat gtgtttttta cctacaaaat agggcataaa
    19141 ataaaatgta gcatacatca aaattagaac tggaaatatt gtaaatagac attcaatctt
    19201 actcataata tttcacctca tacttgtcaa ataaacttga acttagtgac aaaaattctt
    19261 ctttactaat acctgataaa taagattttg ccaccaataa ttccaagtca agtttatttt
    19321 tttgacgagt tgtattatct attttcattg gtagttgagc tacaatagca ccttttcttc
    19381 tatcaatatt taagaatccc tcttctttta actcattgta ggatttgttc actgtatgta
    19441 aatttacacc aatattttct gccatacttc ttacagatgg aagtgattca tttattttca
    19501 attctcctga agctatagcc ttagttattt cttctttaat ttgtacataa atagatttat
    19561 cactattaaa atctaaattt aaaaccataa aaaacctcca ttatgcttta taaatgaaaa
    19621 cgattgttat atacatagta taacataaaa aataataaca aacacaaaat ataagtttat
    19681 attttgtaaa aattgtaatt taaaaaataa cataattttt attaaaataa tacagatact
    19741 atttatagat agaaataaaa attagaaagg agaaaaaata tgttaacata tttaatgaaa
    19801 agacaaatgc ataaaaaaga tgatggaatg agtaaaccaa tgatgattac aggagctgta
    19861 attacaggtg taggagcata tgctgtatac aaaatggtaa aaagccataa atctaattct
    19921 caaatggtca atacaaatta ttatggaaat agcgattatg atgattataa atatgacgaa
    19981 tttgattatg actgtgattg tagagacaca aaacaagatt atgaatatga agaattaaaa
    20041 aaaaaagtaa aagaatttaa ttcaagaaga ataaattgtg aaaattgtcc acatgtttct
    20101 caagaagaaa tgatgcaata tgtaaatagt gttaaagatg taaaggaaga tattgattta
    20161 catgaagatg ctaaaaaaga taatatttac aaagaagatg aatacaaaaa atatgaagaa
    20221 gaataagtaa tattttgtat aactgaacaa atagtaatgt caaattattt aatatctagt
    20281 tttaatatgt aagttgtaac ttaacttgaa agaatcagta tcattcttta gatagttaag
    20341 ttacaactta cattttgttt ttataaattt taatagttct ttgtgactat tttgtaattt
    20401 tagaaatatt ttagtatcca gggtaattgt gtataataaa acttgtatag atagatagta
    20461 tgaaattaag cagatagttt aaaattaaaa tgaaaagaaa ggaaatagtt atgttaaagt
    20521 tgttgatagt tgaagatgat agtactatat cttttggaat aaagtatgct ttagaacaag
    20581 aaggatttag tatagatata tcaaaggatt tatctagtgg aaaagaaatg atttcttcta
    20641 atgaatatag catgatactg ttggatgtta cattgccaga tggaactggt tatgagcttt
    20701 gtaaatatat aagaggattc tctcaggttc ctataatatt cttgactgct tgtgatgaag
    20761 aagtaaatat tgtaatgggt ctcgatatag gtggagatga ttatataaca aaacctttta
    20821 gaattagaga attaatatcc agaataaaag ctgttttaag aagaaaaggt aatacttcag
    20881 aagaaaataa gaaaatactt aaatttggag atttaagtat atatacatta gaagctagag
    20941 tatataaaaa tgataaagaa atatttctga catcagttga atataaactt cttttaattc
    21001 ttatacagaa taaaaatact gttttaagta gaactcaaat attggaaaaa ctttgggatg
    21061 taacttatga ttttgtaaac gacaatactc taactgttta tataaaaagg cttagagaaa
    21121 aaataggtga tgatttatgt gaaccaatct acatagaaac tgtaaggggg attggttata
    21181 aatggatagg aagtgaaaat agtgtttcta tataatccag aagtgaagaa gtttttaagt
    21241 aagtatgtta ctttgatgtt tattgttatc ataatatcta ttggatttag cataatcaac
    21301 gtgtctttaa ctaaagagat ggttgtaaga aataatcaag ctataatagg gactttatcg
    21361 agtaagtatc caaacttaga aagtcagata gtagacataa taactcaagg gaagtctatg
    21421 aagaacatag attatggtca gaaaatatta agtaagtata attatgataa atctattaag
    21481 ataaactctg agcctattat ttctaaatta gttttagaga ctatcaaaat aaatataata
    21541 ttagtatgta taatatttat tttgatattt gtgttggttg ttagatattt tacgtcaatt
    21601 tacaatgatt tatcagatat gactaagtat gtttactata gttcagaggg taagccattt
    21661 gatatgaaaa acaagaatca agaagggcag ataggtcttc taaaaactga actcttaaag
    21721 atgacgacaa ttcttaatga aaaggttgaa cttttaaaaa gagaaaagat atttttaaac
    21781 aatacaattt ccgatatatc acatcaatta aaaactccta tgacttcttt aataatgttg
    21841 aatgatttat tatataatga tgtaccatac gaagtaaaaa tcgatttttt aaataagata
    21901 aaaaatcaat taaatagaat ggattggttg attaaaagca tgcttaaatt atcaaaggta
    21961 gaagcaaaag tgataaattt taaaaaagat aaggtaaaat ttagtgaact catacataga
    22021 gcaatgctat ctatgaaaat acctatggag attaaaaatc aaaaactgac tatagaggga
    22081 aatgataatg tatcttatat tggtgatatt gattggtctg tggaagcact agtgaatata
    22141 atcaaaaatt gtgtagaaca tactcctgaa tttggaaata taattataac ctataaagaa
    22201 aatccattat tttctgaact aataataaaa gatgagggtg aaggtataga taaaaaagat
    22261 ataccacatg tatttaaacg attctataga ggaagaagta gttcaaaaga agatagtgta
    22321 ggaataggtc ttgcaatgtc aaaatctata atagaaagcc aaaatggcga tatatatgta
    22381 aacagcgaaa aaggtaaggg tacagaattt catataatat ttcataaaat gtatgatagt
    22441 gactaatctg taatatttaa ttcactttat agtaatagcc aaaacctaat atatgaagta
    22501 tagaatataa atattggagg tagttatgga aattttaaga gtagaaaatc taactaaaag
    22561 ttatggtaaa aatgaaacaa aagttgatgc tataaaaaat gtaagtttat cagttgaaaa
    22621 aggaacgttt atagcaataa caggtccaag tggaagtggt aaaagtacct tattacattt
    22681 attaggagga gttgacagac caactagtgg taaggtttat ataaatgatg ttgatatata
    22741 taatttaaaa actaaggatt tggcgatatt tagaagaaga aatataggtc ttatatatca
    22801 attttacaac ttaattccag tcttaaatgt gaaggaaaac atattgcttc cagcagagct
    22861 tgacaataga gatattgata gagaatattt tgatgattta atgaaaacat taggtctaat
    22921 tgatagagaa aagcaccttc caaaccaatt atcaggaggt caacaacaga gaacctctat
    22981 aggacgtgca ttaattaata gaccttctat aatattagca gatgagccaa caggtaattt
    23041 agatagtaaa aattcaaagg aaatattaga attgttaaaa ttgtcagtaa aaaaatacaa
    23101 tcagacacta ataatgataa ctcatgacaa aaatatggca cttcaagctg atagaattat
    23161 aagcatagaa gatggaatca taaaaagtga tgaggtgatg tagatgaatt tatatacatc
    23221 actaaccatt agatacttaa aacaaaataa aagaagaacc attgtaacca taatcggtat
    23281 aatactttca acagctttaa tttgtggtat aggaaatata tttgaaagtt ttatggatta
    23341 tcaaatgaga gaaactataa agaatgatgg aagtttccat gttacatttt atgatgtaaa
    23401 tagaaaaaat gtggaatatg taacgaaatc agctgaaata gaaaaacatg catttaccaa
    23461 acaacttgga tattctaaat tagaaaatag tgaaaatgct atattaagta taaagcaata
    23521 tgatcaaaat gcaataaacg gatataaagt ttctataaaa gaaggtagat ttccagctaa
    23581 ggaaggagaa attgttttaa gtgaaagtat tattaatctt atagatgata agttaaaaat
    23641 aggagataag ataactttaa aagtagggga tatgtttgac tcaagcaaga aaaagattga
    23701 tagtatgaca tttcatgaag gtgattatat agcaaatgaa aaagatagaa cttttaaagt
    23761 tgttggtata attaaaaaac ctgggtttga acgttataat ggaattgcaa cagctatagc
    23821 ctactttaat cctatgaata attacaatga tacaattaat gtatcagtag cagtaagaaa
    23881 tcctaaagat gtgtataaga taagtaataa aataagtaaa aatatatatt caaataaaga
    23941 taaagaagca gtttctgata tgataaaata caatgaacat ttattgaggt tacaaggtgc
    24001 tagtaagtat tcaaatataa atagctctat aaagtctata attatagcag taactatttt
    24061 agtaatcata tgtacaattg ctacagtgta caattccttt agtatttcta taaatgaacg
    24121 aaaaaaacag tttgggatat taaattctat aggtgctaca tctagtcaaa ttaaaagatt
    24181 agtttttata gaaggtatta taattagttt aattggtata ccaataggac taatatctgg
    24241 aacagtagct atagacctat tgtttaaaat tataaataag tattttactg aatcggttgt
    24301 tactaagatg agtttgcaga tagtgtataa tccaataata attattgtta gtataataat
    24361 agttttgctt acaatcttta tatcaattct actacctgca atatcggctt ctaatatttc
    24421 tccccttaat gtgattaaaa atactggaga gtataaagtg ggcaaaatca aaagctcaag
    24481 acttattaaa atgattttta aaactgaagg agtacttgca tataaaaata ttaggagaaa
    24541 tagaaaaaaa tttataataa ctcttttttc tttaatggta agtataataa tatttatatc
    24601 ttttagtggc tttacattac ttttactaag aggtgaagaa attagaaatt ctcaaagaaa
    24661 atatgatttg tatttaactg caaagaacac agctaggtct gtagaggata ctataagtga
    24721 actagaagat attgatggga taaaaaactt tgaattggca acaggacaat atgtgtctat
    24781 aagagtaagt gaaaataaaa taaataaatc caaggaagat ttaatcagga aatattatca
    24841 aaaatataag attggagaaa gttatgaata tgatttcagt aataatgaat tgatttttcc
    24901 gggagatttt gcagtaaaaa acaaaataaa taacttagta cagggctctt ttaataaaga
    24961 gagagcaatt gaagaaaatg gtgttatatt agttagaaaa agtgcaattg aagaaaatgg
    25021 gaagaaaggt gtagtagaac ttaccaacta taaagtagga gatacagtaa attgtgaata
    25081 tcttgatgaa aatgccttaa gtaaaaaagt aaaggtcaag attcttgcta taactgatga
    25141 agaacgtcta ggttttggtt atcaaaatat ggggttacaa tttataactt atgatgaagt
    25201 tgctaaaaat ttaaatttaa aattaagtaa aagccttata tttattgatt caggaggaag
    25261 cattaagact aagaaggaaa tagaagcttt ggcaaataaa aatgatttta acttttatga
    25321 tgaaatttca agcagtgaaa gtgaaaaaca agatttaaag gttattaaaa tatttgtata
    25381 tggttttata gtagtgatat ctttagtgag tgttacaaat attctaaata cagtaagtac
    25441 aagtataaat ctaagaaaaa gagaacttgc agtaatacaa tctatagggg ttactcctaa
    25501 aggatttagg aaaatgatat atttagaaag ttttatatat ggtatactgt cattgttgtt
    25561 tggaatacca attagtattg gtataattct aattatgaat aaacttattt caggtgtaat
    25621 tgaattttct ccgattatac cgtggacagc catagttatc tgtatagtaa gtgtttttat
    25681 aataactttt atagcagggt atataccaat gagtaaatta aataaagaaa atataataga
    25741 taata
//
LOCUS       DAMJWP010000036        25361 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.037, whole genome shotgun sequence.
ACCESSION   DAMJWP010000036 DAMJWP010000000
VERSION     DAMJWP010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25361
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.037"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            127..801
                     /locus_tag="PC700_001259"
     CDS             127..801
                     /locus_tag="PC700_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2580575.1"
                     /translation="MNNKILVVDDEEGIITLLKDYFEMNGYEVYTAQSGKDSLKKISK
                     NPDIILLDINMPEIDGIEVCNRIRDYVSCPILFLTARIENTDKINGFRAGADDYIVKP
                     FDLDELGARVSAHLRREQRKSNQTKIKFYNDLTIDYEKRLICIYNNQISLSKKEFDIV
                     ELLSMNPGQVFDKEKIYDRVWGYNSNGNSDVIMEHIRKIRIKLSKHTLENYIETVWGV
                     GYKWIK"
     gene            789..1949
                     /locus_tag="PC700_001260"
     CDS             789..1949
                     /locus_tag="PC700_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2580576.1"
                     /translation="MDKISNMSLKKAFFVLTLSSLIIASVLTTFAYILLNNLYNSIQD
                     KYLKTPINTGIITIVQNNVNQFSDYDKTLLNIINILQLVLPLIFFIGLLLLADIIFYK
                     VKLKTPIEILNKGAMEISNNNLDFCLEYNNNDELGNLCNAFEKMRSELNKNNINMWTM
                     INDRKQLNAAFSHDLRNPLTVLKGYSNYLTKYIPTGKLSDEKILSTTQLMSEHINRIE
                     YYVENMSNAQRLEDLVVSKSMSNINKFIENLDENISIIAKQEGKSFTLKSQINNINLF
                     FDENIILRVVENIISNAFRYARNNVSILIYLEQELLTFVIEDDGIGFSKESLKLALRP
                     FYRDKTLNDSNPHFGMGLHISKILCEKHGGSISIENNSTNSAKITAKFSTRN"
     gene            2166..2693
                     /locus_tag="PC700_001261"
     CDS             2166..2693
                     /locus_tag="PC700_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDF2580577.1"
                     /translation="MKSFANNITFSRIVFALLMLSSTFCSLPFWICYFWCGISDILDG
                     LIARKLRQQSGIGAKLDSIADFIFAVTILVTVGKNIALPMYLWWCIVAIALLRLISYS
                     IGFFKYRTFSSLHTLMNKITGISIFAFPLLYICFGLDVAGIMICIVAFLSSFEEIAIM
                     IKSKELNRDIKSIFM"
     gene            2979..3647
                     /locus_tag="PC700_001262"
     CDS             2979..3647
                     /locus_tag="PC700_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2580578.1"
                     /translation="MIIKAKQLSKIYGSNNNKVIALNNVNLKINSGEFVSVIGPSGSG
                     KSTLLHILSGLDNPTSGQVLLDDKDIYKHTEKELSALRRKSFGFVFQQFNLLPVLTAS
                     ENISMPVLLDKKQPDKDYLNEISSLLGIADRLNHLPHELSGGQQQRVAIARALIAKPD
                     IIFADEPTGNLDSKSGSEVMNLLIKTSKQFGKTLVVITHDDRIAKLADRKISIIDGVL
                     MEVK"
     gene            3650..6118
                     /locus_tag="PC700_001263"
     CDS             3650..6118
                     /locus_tag="PC700_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2580579.1"
                     /translation="MKNYLSLSIKELKSQKLMTTFIIIAIVLSTIMTTVVGQSIGILQ
                     NLRIEQARSFNGDRHVSFHQLTKNQVDDIKKDDRVYQAGTSITIGSSKIKDSGISVLV
                     KEYDKTGLSNYPKLMKLKSGHLPKDKNEIALDENTLKLMGIKPRLGVTIPMNLDISLL
                     NDTIPPYNYTANFKLSGILEDDYTGYVSGIVNGIVGKGTSENLLPKRYILSSLDFKIK
                     QQEKFQEIVNQLAKKINLSHNSIQYNWIYLNALKIQFEKDENSSNSDGISMIILVSLF
                     VALLVLLASGLVIYNILKISVTKKIKEYGCLRAIGAEPNQIYKIVILQILILCTVAIP
                     IGAVIGIISSKGITGMVTNILNPDILLANDNKEITELIHKNTTAYMFPLVLSTNVSLI
                     FSFISALPSAIYASRVSPKIAMAGSTTKIKRKIKREKAIKNFERHLAWLNLKRNKGRT
                     IITILSLFMSITVFVALSEFSNVLDVSRSVSNLKEGDFSLTNEISGFDKSTLDKIDKM
                     KNVNRTSFIKYSEYKQGEIDTDINFENSGEMLKIIGIDEQTLKDLMPSITDSILEDFK
                     NGSICFIKNPIAISTPGVKTRHTNLKPKDSITINKKQLDIYSTVDKMFFLQGNGWING
                     VDVIVYNSVYNTLTNKNKFNQINIYAKDKSKLEQIRLSIEQICENNPGSHWISYIESD
                     KQLKESFKQIEFLAWAVILFVGLIGTLNIINTTHTNINTRTNEIGVKRAIGMSNISLY
                     KMFLWEGVYYGIFAAIFGSIAGYASAIIINMATIEKLDFTNIPITSILQATIISVLAC
                     IIATLIPLRKVKKMNIIDCIDINL"
     gene            complement(6358..7782)
                     /locus_tag="PC700_001264"
     CDS             complement(6358..7782)
                     /locus_tag="PC700_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2580580.1"
                     /translation="MKNQSLITQFRHTFIFIIIASIVATVITYVFALYLYIHSLNKDI
                     YPPNYYERQVPRIEKYINEKNITLLSQSNEEGLKRTIRGDDMPYQVVDNNGNILYGTN
                     PKKLFKTKEELFNNFINKTVRKGGYIHTVPIKGDNGKIEGAVILFYQVKITFANSRGR
                     FVFAVIIMALFSPFIYIVGFTRWLSKRFVKNINQPLHLLIDASKKIKEKDLDFEIDYY
                     SDNELGKLCSAFSEMKDELKGSLSAQWKMEQERVEMVEALAHDLKSPLSIILGYTDAL
                     IGNNTDDNEKLHRYLTVIRENTEKSAALVQKMQYTSDLEKSNIQLNLVPINLPEFLRQ
                     KVQDYELQAHQKEVELILKMKGNIQSPIQIDVDRLTRIFDNIISNSLQYTPSGGNISI
                     TVKDEKNCISYEICDSGRGFSSKDLKKALDKFYRGDEARQTKGGHSGLGLYIVKQLVE
                     QLGGSVKIENSKSGGACVKFWHSI"
     gene            complement(7801..8493)
                     /locus_tag="PC700_001265"
     CDS             complement(7801..8493)
                     /locus_tag="PC700_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2580581.1"
                     /translation="MSKILIIDDEMDLVMLLEDELKAKGHEVLIAYDGQAGIELSKQE
                     PDLIILDIMMPKMNGFEVCQAIRDDVLCPIIFLSAKQSETDKIKGLTFGGDDYVTKPF
                     GLRELMARIEANLRREKRSQYINEENKRSTLYFGKLCLYMKERAIKIDGKNIDLTKIE
                     YDIVELLALHAGQVFSREQIYEKVWGCDSEGDSSTVVERVKKIRAKFSAVTPEKEYIS
                     TVWGIGYKWNKM"
     gene            complement(8548..9324)
                     /locus_tag="PC700_001266"
     CDS             complement(8548..9324)
                     /locus_tag="PC700_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HDF2580582.1"
                     /translation="MIKLLSSEWLRAKRTAVQWLTLCMPIIFSLCVVFYLTTKSGSTQ
                     EFAFEGFFTVWTVFIIPIGVGILAGFIVQEEELAGNFNGFLCVGISRVRLYLGKFLFL
                     LFCLTICTFIATLILCIGMSIAVPSGATIWLFLSAAGLVVIGTLPLLAIHLWVSFAWG
                     MGASIGISIGGILMAAILGLTSVGEKVWAFVPWTWPVKLGMLPGIYFIKEAGTISTEA
                     FYSKVMQTASIELIVVAIGLIIFLIGGVIWFKMWEGRKSY"
     gene            complement(9321..10091)
                     /locus_tag="PC700_001267"
     CDS             complement(9321..10091)
                     /locus_tag="PC700_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HDF2580583.1"
                     /translation="MNALQSELLKYKRTFMGKLIVFFPVSFAAYAFIMQSTLMQNPLS
                     QTTSWAWQSLLALIFNWWSFLFLPIGFALFATLVAFQEKKAGNYRALRTHNVSPMTLW
                     INKVIAMAVYSFISTLVLIVVTIITGLILKAGPVPFGQIIGASIVCWVVSLAILPLQL
                     WLATWKGMFLSMGVGALGMIFGVLAATKPFWIAVPWSWAVRMVCPIINIHPNGTILEA
                     GDPLLNTSVIPIGIVVSLVVFIVLTALTAAWFNRRDDK"
     gene            complement(10088..10792)
                     /locus_tag="PC700_001268"
     CDS             complement(10088..10792)
                     /locus_tag="PC700_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HDF2580584.1"
                     /translation="MKDLILETKNLSKRYGEQMAVNNVSLQIERNSIYGLLGPNGAGK
                     STTLKMLVGLLRPTDGRIIFDRKPWKRESLAKIGSLIESPALYGNLTAEENLLVHTKI
                     LGIPKDKIYEVLEIVNLKNTGKKRASQFSMGMKQRLGIAIALLSDPELLILDEPTNGL
                     DPFGIQELRELIASFPKKGITVILSSHILSEVAQVVDNIGIINGGRLLFQGMPDPNEN
                     LEEFFTDVILKGGQNR"
     gene            complement(11047..11559)
                     /locus_tag="PC700_001269"
     CDS             complement(11047..11559)
                     /locus_tag="PC700_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580585.1"
                     /translation="MKKISQEKISTHIQEVPQEKIEQWKSLYEKYKSILKVNSKSVLE
                     IIEFLKRKYVIVEETSEEFKHVVINNIKLNKPFSQKISSDKELKPIVFTIPNEGNAKK
                     LYDTQEETYSGCKIIVGMEFETGCVFVEGSDELNDEITSFKGLDKYDISNYYLVANYI
                     RCLKKYKLID"
     gene            11885..12142
                     /locus_tag="PC700_001270"
     CDS             11885..12142
                     /locus_tag="PC700_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580586.1"
                     /translation="MILNRTELFRGYSLNRFNEICNILSVNKIKYKYKVKNNAYSKKP
                     FSNEITLGNLGQKEDFSYEYLIYVHKDDYEQAESLINSKLR"
     gene            12285..12911
                     /locus_tag="PC700_001271"
     CDS             12285..12911
                     /locus_tag="PC700_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HDF2580587.1"
                     /translation="MKINIKSKLSEFIKQNNLFDDNRIISYLPNITIETDKIKTIMIN
                     EVVPTNTNDDFYSVDKDADYLKTTIPLFDSAGIEVSNINDILDMGIYITNAVKLPKSE
                     YTITRDTIKLHMPILEEEIKLFKNLEVVMLMGDVAKKSFNMITKKHIKKNVIPSISTY
                     KLRNNELYYDNLRVFPSYIMTGGNILIEKSKFEMASEDIKKMFEIING"
     gene            complement(13161..13940)
                     /locus_tag="PC700_001272"
     CDS             complement(13161..13940)
                     /locus_tag="PC700_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901940.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HDF2580588.1"
                     /translation="MNSKIYYFSGTGNSLVVSKILKETLNGSKEVIPLAIHRKENNID
                     INEDILVIVFPVYFADVPDIIKSFVEKLNFNINTHIYAIATCNGVAGHSLFTIDKLLK
                     KRGKKLSAGFLINMPGNALITPSDVEEERLKNQTNRVAEIAKCINNLEIGNIEGKNNL
                     KSHIDSVVLRLVGKNMQIATTKFFAENKCNGCGTCKRVCPVENISIVDKKPKWGNECE
                     RCLACFHWCPKEAINVKKSFLTNRKKYHHPDITVKDIFLDK"
     gene            complement(14008..14469)
                     /locus_tag="PC700_001273"
     CDS             complement(14008..14469)
                     /locus_tag="PC700_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2580589.1"
                     /translation="MGGAGDYLNGGKMKDFEKLYEATTKLVSTSLKVKDDLKKMFKQN
                     GYDITTDHYALLRFLWEQDGISQIDLCEKSCKDKSNTTRILDVMKNKGLIVRKVDVKD
                     RRKFQIFLTDLGRELEEPLNEIASIYATETFKSISDEELPLFTDILDRIGK"
     gene            complement(14445..15122)
                     /locus_tag="PC700_001274"
     CDS             complement(14445..15122)
                     /locus_tag="PC700_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2580590.1"
                     /translation="MIITVINGSPRKNGATSKVLTYLYKDIERLIPDVKINYFDLSEV
                     NPSYCIGCLNCYKMGKCINQNDKVEYIHDIITKSDGVIFGSPTYGSSVTGLFKVFTDR
                     AHMMLERLLYRKPCIAVTTYENARGSKAISFIKSMVLDSGGYVCGSLSIKTGFNQNPI
                     TEKVESKIQKVSKKFIYCIEEKKNPPVLSQIYNFIAINAVLKPMAFKDIEQYKGIIDR
                     WEEQGII"
     gene            15355..15741
                     /locus_tag="PC700_001275"
     CDS             15355..15741
                     /locus_tag="PC700_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2580591.1"
                     /translation="MKKLPHISEAEYQVMKIIWKYAPISTTEVIEKLVETSTWSPKTI
                     QTMLLRLVKKGALTYEKNSRVFVYTPLVKEEEYVATESSSFLNRFYNGTLNSMVLNFL
                     ENDKLSEDDIEELREILNKRTTKGDK"
     gene            15744..17543
                     /locus_tag="PC700_001276"
     CDS             15744..17543
                     /locus_tag="PC700_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaR1 family beta-lactam sensor/signal
                     transducer"
                     /protein_id="HDF2580592.1"
                     /translation="MYAPFFIRFLLSTLTLSILIVIILLAKKVFKKHISAKYQYNIGF
                     ILLIMLIIPFVPLKYINLGNVFNYLYTFNQLGSSTNRTSIIKNSPNSIINNSNALNDF
                     SVSLNQVNFDSLNILLLISWIIGILIMIAITIHCNIKIKNMKKSIQILRDEEIVFIFE
                     KCKKDIGINKDFILCKSPIINTPITFGYFKPHIILPDKSISKLSLKDIKYILLHELQH
                     FKNKDILINYIMCFLQILYWFNPLVWYAFKEMRIDREIACDISVLKKLDKDSHIEYGQ
                     TLINFVDNISRPSNLTLTSDIGGSKKQIKKRLLKIMDFHNESNTLKFKSILIFTLIAL
                     IVFGNIPKLSIMADINEKYRPTNEQTVYEDLSQYFKGFDGSFVMYDLKSKQYHIYNKE
                     KSKTRISPNSTYKIYSALLGLENGIITRNNSHIPWDGKHHSNSSWNEDQDLFSAMENS
                     VNWYFQTLDKKTGLKNIQTYLKQIGYGNYDVSGGISNFWLESSLKISPIEQVELLESF
                     YTNKFKFEDKNIETVKDSLLISKKDGVSLFGKTGTANINGKDVNGWFIGYVEKNENTY
                     FFATNIQSKDYSNGSSASKITLSILNDKKIYNP"
     gene            complement(17898..19772)
                     /locus_tag="PC700_001277"
     CDS             complement(17898..19772)
                     /locus_tag="PC700_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2580593.1"
                     /translation="MNKYNKIANELIEIIGENNIISITHCATRLRVMVKDREIINDKK
                     VEKVNEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLFGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEVEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIGLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLVSIFVVA
                     FCFIVTCLFGVPKEAINEDEDEELVFNENNENFVSPAKGKIVALENVPDETFSKKMLG
                     DGFAIDIIDGKIVSPISGKLETVFSSGHAFGIKGTNGEVLIHVGIDTVALNGDGFDIA
                     VKQGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE
                     "
     gene            complement(19777..21441)
                     /locus_tag="PC700_001278"
     CDS             complement(19777..21441)
                     /locus_tag="PC700_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDF2580594.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIISRLDYLKDLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKESKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKTVRNQPGIEKMLYELKENTFKKYDCMTVAEAAGISYDQLGDFIGEDGFFSMV
                     FDFKHADLDVASGSEWFKRISWTIPDLRKCILDSQMYVQEHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRDTINDFNDLSSIDQYY
                     RSIDEGYSEEEALEFVNLRSRDNARTPFPWADKENAGFSDATPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSKYSDCLIYGSIEPIDIESDNVIAYRRQLDLVVIDCY
                     YNFSDKLVEIKTEEKYDIVFQNLDDINLLNKTVSLKPFQAVLLKVR"
     gene            complement(21455..22276)
                     /locus_tag="PC700_001279"
     CDS             complement(21455..22276)
                     /locus_tag="PC700_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2580595.1"
                     /translation="MKLYKLLILDIDGTLRDEVYGIPESAKHAIRLCQKNHCSVVICT
                     GRSIGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKEVVCLLKKREVAF
                     SIESQEKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDVHKIC
                     LWSNEKVFDEVKDILQDKMELAQRDISSQYYEIIQKDFHKGKAIKRLQERLGVTQKET
                     ICFGDGQNDIVMFQASDVTIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            complement(22375..23139)
                     /locus_tag="PC700_001280"
     CDS             complement(22375..23139)
                     /locus_tag="PC700_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2580596.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVIDDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVVKEMKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDTSTIIDFAKEMRMKGTYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            complement(23136..23885)
                     /locus_tag="PC700_001281"
     CDS             complement(23136..23885)
                     /locus_tag="PC700_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2580597.1"
                     /translation="MRLESLVNQSYNSLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYDAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KSDLFIIISLSGETKSGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
     gene            complement(24389..24994)
                     /locus_tag="PC700_001282"
     CDS             complement(24389..24994)
                     /locus_tag="PC700_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2580598.1"
                     /translation="MKIKENVLMLDSTRGSNCFIVFDKEIILIDTGLPFMGKNIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIPFIIGEKDRPGFKK
                     FIGKAISRRLIKDVEPYGENMKIGNIKIIHTPGHTPGHVCMIFEDVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMMKSIRELDNLKYEWICMSHGTPCIK"
     gene            25173..25271
                     /locus_tag="PC700_001283"
     CDS             25173..25271
                     /locus_tag="PC700_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDF2580599.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFD"
BASE COUNT         9095 a         4090 c         3099 g         9077 t
ORIGIN      
        1 atatataaaa atatactaac tttaatgatt aagttttgaa atataactaa gaatttgaga
       61 atttattgag atttttatac taaaatataa atactataca aacttaattt agaagggagc
      121 attcttatga acaataaaat actcgtcgta gatgatgagg aaggtataat aacactctta
      181 aaagattact ttgaaatgaa tggttatgag gtttacactg ctcaaagtgg aaaagactca
      241 ttaaaaaaaa tatccaaaaa tccagatatc attctacttg atataaacat gcctgaaata
      301 gatggaattg aagtttgtaa tagaattaga gattatgtat catgccctat tctatttctt
      361 acagccagaa ttgaaaatac tgacaagata aatggttttc gtgctggtgc tgatgattat
      421 attgtaaaac cttttgattt agacgaactt ggagctagag tatctgcaca tttaagacgt
      481 gaacaaagga agtcaaatca aactaaaatt aaattttata atgatttaac aatagattac
      541 gaaaaaaggt taatttgtat atataataat cagatttcac tttcaaaaaa ggaatttgat
      601 atagtagagc ttctctctat gaatcctgga caggtatttg ataaagaaaa aatatatgat
      661 agagtctggg gatacaatag caatggaaat agtgatgtta taatggaaca cattagaaaa
      721 atacgaatca aactatcaaa acatactctt gaaaattata tagaaacagt ctggggagtt
      781 ggttataaat ggataaaata agtaatatgt cattaaaaaa agctttcttt gttcttactt
      841 tatcttcact aattatagca tcagtactta ctactttcgc atacatttta cttaataatc
      901 tatataattc tatacaagat aagtatttaa aaacacctat aaatacaggt attataacca
      961 tagttcaaaa caatgtgaac caatttagtg attatgataa aacattactt aatataatca
     1021 acatacttca gcttgtactt ccacttatat tttttatagg acttttatta ttagctgata
     1081 taatttttta caaggtaaaa ttaaaaacac ccatagaaat cctcaataaa ggtgcaatgg
     1141 aaatatccaa caacaattta gatttttgct tagaatataa taataatgat gaattaggta
     1201 atctatgtaa tgcttttgaa aaaatgaggt ctgaactaaa taaaaacaac ataaatatgt
     1261 ggactatgat taatgataga aaacaactta atgctgcttt ttctcatgat ttaagaaatc
     1321 ccctaacagt tctaaaaggt tactcaaact atttaacaaa atacatacca acaggcaaac
     1381 ttagtgatga gaagatttta tcaaccacac aacttatgtc tgagcatatt aacagaattg
     1441 aatactatgt agaaaatatg agtaatgctc aacgattaga agatttagtt gtttcaaaat
     1501 ctatgtcgaa tataaacaag tttatagaaa acttagatga aaatatatct atcattgcta
     1561 aacaagaagg aaaatctttt acacttaaaa gtcaaataaa taatataaat ctattttttg
     1621 atgaaaatat cattcttaga gtcgtcgaaa atataatatc gaatgctttt agatatgcta
     1681 gaaataatgt atccatactt atatatcttg aacaagaact ccttaccttt gtaattgaag
     1741 atgatggtat aggatttagt aaggaatctc taaaattagc tttaagacct ttctatagag
     1801 ataagacttt aaatgacagc aatccacact ttggtatggg tcttcatatc tcaaaaatat
     1861 tatgtgaaaa acatggtggc tctatttcta ttgaaaataa ttctactaac agtgctaaaa
     1921 ttactgctaa attctcaaca agaaattaaa atgattttgt aagctttcat tgttcttata
     1981 taagctttgt tagtttagaa tttataaaat gtatttcaat ttacaaccaa tatatatact
     2041 attttgtaga agttgtaaga gttgcttcaa ttataatatt ttggcaaaag gcatttgaaa
     2101 caataataga aatatatatt taaatatgta gaatagtatg aaactagaat tttggaggtg
     2161 attatattaa aagttttgct aataatatta cattctctcg tattgtgttt gcattgctaa
     2221 tgcttagttc tacattctgt tcactgcctt tctggatatg ttatttttgg tgtggaatta
     2281 gtgatatact tgatggtctt attgcccgaa agttgagaca acaaagtgga ataggtgcaa
     2341 agttagatag tattgctgat ttcatttttg cggttacaat tttggttact gtcggaaaaa
     2401 atattgctct tcccatgtat ctatggtggt gtattgttgc aatagcatta ttacggttga
     2461 ttagttacag tattggtttt tttaagtatc gcacattttc atcgcttcat actttgatga
     2521 ataaaatcac tggaatttct atctttgctt ttccattact ctatatttgc tttggtcttg
     2581 atgttgcagg aattatgata tgtatcgtgg cttttctttc ttcttttgaa gaaatagcaa
     2641 ttatgataaa atcaaaagaa ttaaatcgtg atataaaaag tatatttatg tgacaactta
     2701 gaatttgtga caaaaataat ttatataata atgaggaagt aaaacataaa aaattatcca
     2761 caagtttatg tgtaaaatag ttttaaagca atagagttgt attgtaaagc ctttagtgca
     2821 taaattacat tttaaattaa aaatgatact gaaactgctt atcagcactg cgaattatcc
     2881 gtaaatagtg aagacttttt agatttaagc taaaagttgg gaaaaaattg agttttaaaa
     2941 cttacactct tcttatatag aaaaggagag tgttttttat gattataaaa gcaaaacaat
     3001 tatcaaaaat atatggttct aataacaata aagttatagc acttaataat gtaaatttaa
     3061 aaataaactc aggtgaattt gtttctgtaa ttggaccttc tggaagtgga aaaagcacac
     3121 ttttacatat tttaagtgga ttagataatc ctacctctgg acaagtgtta cttgatgata
     3181 aagatatata taaacacact gaaaaagaac tatctgcact tagaagaaaa tcctttggat
     3241 tcgtctttca acaatttaat cttttacctg ttcttacagc atctgaaaat atttcaatgc
     3301 ctgtacttct agataaaaaa caacctgata aagactattt aaatgaaatt tcatcattac
     3361 ttggaattgc agatagactc aatcatcttc cccatgaact ttcaggcggt cagcaacaaa
     3421 gagttgcaat cgctagagca cttattgcaa aaccagatat aatatttgct gatgagccta
     3481 caggaaactt ggacagtaaa agtggcagtg aagttatgaa tcttcttata aaaacttcaa
     3541 aacagtttgg aaagacttta gtggtaatta ctcatgatga tagaattgca aaacttgctg
     3601 atagaaaaat ttcaattatt gatggtgttc ttatggaggt gaaatagaaa tgaagaatta
     3661 cctttcactt tcaataaaag aattaaaaag ccagaaactt atgactacat tcatcattat
     3721 agcaatagta ctatcaacta ttatgacaac tgtggttgga cagtcaattg gtatcctaca
     3781 aaatttaaga attgaacaag cccgttcttt taatggtgat agacatgtat cctttcacca
     3841 acttactaaa aatcaagttg atgacataaa gaaagatgat agagtttatc aagctggtac
     3901 ttcaataact attggcagta gtaaaataaa ggattctggt atctctgtac ttgtaaaaga
     3961 gtatgataaa acaggacttt ctaactaccc aaaattaatg aaattaaaat ctggacattt
     4021 gcctaaagat aaaaacgaaa tagccttaga tgaaaacact ctaaagctta tgggtataaa
     4081 accaagacta ggagtgacta tacctatgaa tttagatatt tcacttttaa atgataccat
     4141 accaccatat aattacacag ctaactttaa attgagtggc attttagagg atgattatac
     4201 aggatatgta agtggtatag taaatggtat tgtaggtaaa ggtacatcag aaaatctatt
     4261 acctaagaga tatattttat cctctttgga ttttaaaata aaacagcaag aaaagtttca
     4321 agaaatagta aatcaattag ctaaaaaaat aaatctttct cacaattcaa ttcaatataa
     4381 ttggatttac ttaaatgctc ttaagataca gtttgaaaaa gatgaaaata gttctaatag
     4441 tgatggtata tctatgatta tattggtatc tttatttgtt gccttattgg ttttactggc
     4501 ttctggactt gtaatctata atattttaaa aatctctgtt acaaagaaaa ttaaagagta
     4561 tggatgttta agagcaatag gagctgaacc aaaccagata tataagatag ttattttaca
     4621 aatcttaatt ttatgtactg ttgccatacc tattggagct gtaattggaa ttatctcttc
     4681 taaaggcata actggaatgg tcactaatat cttaaatcca gatatacttc tagcaaatga
     4741 taataaagaa attactgagc ttatacataa aaatacaaca gcatatatgt tcccattagt
     4801 attaagcact aatgtatctt tgattttttc atttatttca gcattaccat cagcaatata
     4861 tgcttcacgt gtctctccca aaattgctat ggctggtagt actaccaaaa tcaaaagaaa
     4921 aattaaaaga gaaaaagcaa taaaaaactt tgaaagacat ctagcatggc taaacttaaa
     4981 aagaaataaa ggaagaacta taataactat actatcatta tttatgagta taacagtatt
     5041 cgttgcattg agtgagttct ctaatgtatt agatgtatct aggtcagtaa gcaatttaaa
     5101 agaaggagat ttttccttaa caaatgaaat atctggtttt gataaatcaa cactagataa
     5161 aatagataaa atgaaaaacg tcaatcgcac ttcctttata aagtactctg aatacaaaca
     5221 gggtgaaata gatacagata taaattttga aaatagtggt gagatgttaa aaatcattgg
     5281 aattgacgag cagactttaa aagacttaat gccttctatt acagattcta tactagaaga
     5341 ctttaaaaat ggttctatct gttttataaa aaatccgatt gctatttcta caccaggtgt
     5401 aaaaactaga catacaaatt taaaacctaa agatagtatc acaatcaaca aaaaacaact
     5461 agatatatat agtacagtag ataaaatgtt ctttttacaa ggtaatggct ggataaatgg
     5521 tgttgatgtt attgtatata attctgttta taacaccttg acaaataaaa acaagttcaa
     5581 ccagataaat atttatgcta aagataaatc aaaattagaa caaataagat tgtctattga
     5641 acagatttgt gaaaataatc ctggctctca ttggatatca tatattgaat cagataagca
     5701 acttaaagag agttttaaac aaatagaatt tctagcatgg gcagttatat tatttgtggg
     5761 attgattggt actttaaaca tcattaacac aactcataca aatataaata ctaggactaa
     5821 tgagattgga gttaaaagag ctattggtat gagtaatatt agcttatata aaatgttttt
     5881 atgggaaggt gtttattatg gaatttttgc tgctatattt ggttcaattg ctgggtatgc
     5941 cagtgctatt attataaaca tggcaactat agaaaagctt gatttcacta atataccaat
     6001 tacttcaatt ttacaagcta ctattatttc tgtacttgca tgtattatag ctactcttat
     6061 tccgttaaga aaagttaaaa agatgaatat aattgactgt atagatatca atttataact
     6121 attttacaga taataatata aattaaatta tcaatgactg tctctatttt ctttcaaatc
     6181 taactaataa atctttttaa ctataatata agctttaaaa aaggcctgtc tcaaaataga
     6241 gataagcctt ttttcattgt cggaataatg aaaatatatt taacttcaca aaaaaagagt
     6301 gcctcacgaa ttaaaaattt cgctttgaga cactcccctt taatttaaaa tatatagtta
     6361 aatgctatgc caaaatttca cacatgctcc acctgatttt gaattttcta ttttaacaga
     6421 acctccaagc tgttccacta attgtttgac aatgtaaagc ccaagaccag aatgtccacc
     6481 ttttgtctgc ctagcttcat ctcctcgata aaacttatca agagcctttt ttaaatcttt
     6541 agagctaaac cctctaccag aatcacaaat ctcataagaa atacaatttt tttcatcctt
     6601 tacagtaata ctaatattac cacctgatgg agtgtattgc agactgttgg atatgatatt
     6661 atcaaaaatt cgtgtcaacc tatccacgtc gatttgaatt ggggattgta tattcccctt
     6721 cattttcaat ataagttcaa cttccttttg atgagcctgt aattcatagt cttgcacctt
     6781 ttgcctcaaa aattcaggta aatttatagg aacaagattt aattgtatat ttgacttttc
     6841 taagtcagac gtatattgca tcttttggac aagagcagca cttttttctg tattttctct
     6901 tatcactgta agatacctat gaagtttttc attgtcatct gtattattac caataagtgc
     6961 atctgtatat cccaaaataa tagaaagagg agattttaaa tcatgtgcta gagcttccac
     7021 catttcaaca cgttcctgct ccatcttcca ctgcgcagat aaagaacctt ttaattcgtc
     7081 tttcatttca gaaaaggcac tacaaagttt accgagttca ttatctgaat aatagtcgat
     7141 ttcaaaatca aggtcctttt cttttatctt cttggacgca tcaatcaata gatgtaatgg
     7201 ttgattgata ttctttacaa agcgcttaga aagccatcta gtaaaaccca cgatatatat
     7261 aaatggagaa aacagagcca ttataataac tgcaaataca aatctccctc tactatttgc
     7321 aaaggttatt ttgacttgat agaatagaat aacagctccc tcaatctttc cattatcccc
     7381 tttgattgga acagtatgga tataaccacc tttacgaaca gttttattga taaaattatt
     7441 aaataattct tcttttgttt taaatagttt tttggggtta gtaccatata gaatattgcc
     7501 attattatcc acaacttgat atggcatatc atccccacga atcgttcttt ttaaaccttc
     7561 ttcattcgat tgtgataaca aagttatgtt tttttcattg atgtattttt caattctagg
     7621 aacttgtcgc tcataatagt ttggtggata gatatcctta tttaaggaat gaatatacaa
     7681 atagagagca aatacataag taatcactgt ggcaactata ctggctatta taataaaaat
     7741 aaaagtatgt ctaaattggg taataaggga ttgatttttc atttaataac ctctctctct
     7801 ttacatttta ttccacttat acccaatacc ccaaactgtg gaaatatatt ctttttctgg
     7861 tgtcacagca gaaaacttag ctcgtatttt ttttactcgt tcaacaacag ttgaggaatc
     7921 accttcagaa tcacaccccc atactttttc atagatttgt tctcttgaga aaacttgtcc
     7981 tgcatggagt gctagtaatt caacaatatc atactctatt tttgttaaat caatattctt
     8041 accatcaatt ttaattgcac gttccttcat atataagcat aacttcccaa aatacaaagt
     8101 agatctcttg ttctcttcat tgatatactg tgaacgcttt tccctacgca gattagcttc
     8161 aatccttgcc ataagttcac gcagtccaaa tggctttgtt acataatcat cccctccaaa
     8221 ggtcaaccct ttgattttgt cggtttctga ttgctttgca cttaaaaata taattgggca
     8281 taaaacatca tctctaattg cctgacatac ctcaaatcca ttcattttag gcatcataat
     8341 atccagtata ataaggtcag gctcctgttt tgataactct atacctgcct gaccatcata
     8401 agctatcaac acttcatgtc cttttgcctt caattcatcc tcaagtagca ttactaggtc
     8461 catttcatca tcaataatca atattttact caatataaca ccacctttct cttatgggaa
     8521 ggtcagtatt aaaacaatat tcgtctacta ataacttttc cttccctccc acattttaaa
     8581 ccaaataacg cctcctatca agaatataat caaacctatt gctactacta taagctctat
     8641 actagctgtt tgcatcactt tagagtaaaa tgcctcagtt gaaatagttc cagcttcttt
     8701 aataaaataa attcctggaa gcatacctag ttttactggc catgtccatg gaacaaatgc
     8761 ccatactttt tcaccaacgc tagttaatcc aagtattgct gccatcaaaa ttccaccgat
     8821 actaatacca atagatgcac ccatacccca tgcaaaactc acccaaagat gaatagctaa
     8881 tagtggaagt gtcccaatca ccacaagtcc tgctgctgac aggaataacc atattgttgc
     8941 tccagatggc actgcaatac tcattcctat gcaaagtatc aacgtagcaa taaatgtgca
     9001 tatagttagg caaaacagaa gaaaaagaaa ttttcccaga tataatctaa cacgagaaat
     9061 acctacacat aaaaaaccat taaaatttcc agcaagctct tcctcctgca ctataaatcc
     9121 tgctaaaata cctactccaa taggaatgat gaatacagtc catactgtaa aaaatccttc
     9181 aaaagcaaat tcttgtgtac ttccagattt agttgtgaga taaaaaacca cacataatga
     9241 aaaaataata ggcatacaaa gagtaagcca ctggacagct gtcctctttg ctcgaagcca
     9301 ttctgatgat aatagcttta tcatttgtca tccctcctat tgaaccaagc tgcagtaagc
     9361 gctgttaata ctataaacac aaccaaagaa acaacaatcc caataggaat cactgaagta
     9421 ttcaaaagtg ggtctccagc ttctaatata gtaccgttag gatgtatgtt gatgattggg
     9481 cataccattc taactgccca actccaaggt actgcaatcc aaaatggttt tgtcgcagca
     9541 aggacaccaa atatcatacc taaagctcca actcccatac ttaggaacat acctttccat
     9601 gtagctagcc atagctgaag tggaagtata gcaagagaaa caacccagca tacaatactt
     9661 gcccctataa tctgtccaaa aggaactgga ccagctttca atattaaacc agttataatt
     9721 gttacgacta ttaaaactaa cgttgaaata aaactgtaga ctgccatagc tataacttta
     9781 ttaatccaaa gtgtcatagg agacacattg tgtgtacgta aagcacgata atttccagct
     9841 tttttttcct ggaaagctac caaagtagca aacaatgcaa atccaatagg cagaaataaa
     9901 aatgaccacc agttaaaaat taatgctaaa agactttgcc acgcccaaga agttgtttga
     9961 gataatggat tttgcatgag tgttgattgc attataaatg catatgctgc aaaagacact
    10021 gggaaaaaaa caataagttt acccataaat gttcttttat actttaaaag ttctgattgt
    10081 aaagcattca tctgttttgt cctcctttaa gaataacatc tgtaaaaaat tcttctagat
    10141 tttcattagg gtcaggcatt ccttgaaata gtagtcgtcc tccatttata attcctatat
    10201 tatctacaac ttgagcaacc tctgatagaa tatggctaga aagaattaca gttatacctt
    10261 tttttggaaa agaagcaatc aattcacgca attcttgaat gccaaagggg tcaagaccat
    10321 tggttggttc atctagtatt aacaactctg ggtcactcaa aagtgcaata gctattccta
    10381 acctttgttt catacccata gaaaattgtg aagctctctt tttacctgta tttttcaaat
    10441 ttactatttc caacacttca taaattttat ctttaggtat tcccaatatt tttgtatgta
    10501 ctaacaaatt ttcctctgct gtaaggtttc catacaatgc aggggattct attaaagaac
    10561 ctatttttgc aagagattct cgtttccaag gctttctatc aaaaataata cgaccatctg
    10621 ttgggcgaag aagaccaaca agcattttaa gtgttgtgga ctttcctgct ccatttggtc
    10681 ctaaaagtcc atatatacta tttcgttcaa tttgtagaga aacattgtta actgccattt
    10741 gttctccata tctttttgat aagttttttg tttcaagaat taaatctttc atattaattt
    10801 ctcccttctt gtaatacctt aaatctatta ctaagagaat actaacatga aaaaataaca
    10861 attctataac acctactaat tttaataaaa attaaaataa aaaatagagt taccaatttt
    10921 attttggcaa ctctatttaa gtttatgttt tacagttata ttagatttaa gcttagtttc
    10981 attatcacac taacttcaaa tttactctat aatccttaaa tcaagcacta atttattaat
    11041 caaacactaa tctattaatt tatatttctt tagacatcta atatagtttg ctactaaata
    11101 ataattactt atatcatatt tatctaatcc tttaaaagaa gttatttcat cattaagctc
    11161 atcactacct tctacaaaaa cacaacctgt ttcaaattcc atacctacaa taatcttaca
    11221 gccactataa gtttcctctt gagtatcgta caattttttt gcatttcctt catttggaat
    11281 tgtaaataca ataggtttta attctttatc actggaaatc ttttgtgaaa aaggcttgtt
    11341 taacttaata ttattgataa ctacatgttt aaattcctct gaagtttctt ctactattac
    11401 atattttcgc tttaaaaact ctattatttc taaaacagac ttagaattta cttttaaaat
    11461 acttttatat ttttcatata aagatttcca ctgttcaatt ttttcctgtg gtacttcttg
    11521 gatatgtgta cttatttttt cttgagatat ttttttcaat taaactctcc tttcaatact
    11581 taaatatcct tataaatctc acatgactaa agttacaagg ttcctactta atagaatttt
    11641 gcatacttaa atatgtctta ttaattctct acaggatatc tccgtagtcc ctacgattct
    11701 tacataagtt acctatttta aattgttatt agttagatta taaaaattat ttattttatc
    11761 actaatataa tactcttaaa aactttacaa atcaatttaa atttgctacc ttttatttat
    11821 aacaacttat agtacatttc tcctatgctt ttcaatatat tacttttaaa aggaggtttt
    11881 ccttatgatt ttaaatagaa ctgagctttt ccgaggatat tctttaaata gatttaacga
    11941 gatatgcaat atattatcag taaataaaat taaatataag tataaagtga aaaataatgc
    12001 ttactctaaa aaaccttttt ctaatgaaat aacacttggc aatttaggtc aaaaggaaga
    12061 tttctcttat gagtacttaa tttatgttca taaagacgat tatgaacagg cagagtctct
    12121 aataaattct aagttgcgat agattattta taaaacatga cattctgttg tcatgtttta
    12181 tatgttatta tactttcaaa tatgctacta tattttcaaa attgtgtgct ttaaaactag
    12241 ctttttatag taatttaaag cttaaaacta aggagactcg ttatatgaaa ataaatataa
    12301 aatcaaagtt aagtgaattt attaaacaga acaatctttt tgatgacaac agaataatat
    12361 cctaccttcc taatataaca atagaaacag acaaaataaa aactataatg attaatgagg
    12421 ttgtcccaac taacaccaat gacgattttt atagtgtaga taaagatgct gattatttaa
    12481 aaactactat tcctttattt gacagtgcag gaatagaagt atctaacata aatgatatcc
    12541 tagatatggg tatctatata actaatgcag taaaacttcc taaatcagaa tatacaataa
    12601 ctcgtgatac aattaaatta catatgccta ttttagaaga agaaattaaa ttatttaaaa
    12661 acctagaggt agtaatgtta atgggagatg ttgcaaaaaa atcttttaat atgattacta
    12721 aaaagcatat aaaaaagaat gtcatccctt ccatttcaac atataaactt cgtaataatg
    12781 aactttatta tgataattta agagtatttc catcatatat tatgactggt ggaaacatac
    12841 tgattgaaaa atcaaaattt gaaatggcat cagaagatat aaaaaaaatg ttcgagatta
    12901 taaacggttg atattacaaa ataataaaaa ctaatttagc caaataataa gtgcctcaaa
    12961 aacttctatt tatgaggcac ttttatttta tattagtgta actatatact attaacataa
    13021 tttattattt atattgttct ttttaatctc tattccattt agtctaggta acattactct
    13081 ttacaagatt actttatatt tcttttatat ctctaacgtt tttatttaat aaaacaatta
    13141 ttaatacaag tcaaatatat ctatttatct aaaaatatat cttttacagt aatatctgga
    13201 tggtgatact tcttcctatt cgttaaaaaa gattttttta cattaatagc ctcttttgga
    13261 caccagtgaa aacaagctag gcatctttca cactcattac cccactttgg ctttttatca
    13321 actatactta tattctctac aggacaaacc cttttacatg tcccacatcc attacattta
    13381 ttttctgcaa aaaatttagt agttgcaatt tgcatattct tacctactaa acgaagtaca
    13441 acactatcta tatgtgactt taaattgttt tttccttcta tatttccaat ctccaagtta
    13501 ttgatacatt ttgcaatctc agctacccta ttagtctgat ttttcaatct ttcttcttca
    13561 acatctgaag gtgttatcaa agcatttcca ggcatattga ttaaaaaacc agcagataat
    13621 tttttccccc tttttttaag taacttatct atagtaaata aactatgtcc agctacccca
    13681 ttacaagtgg caatggcata tatatgagta ttaatattaa aatttaattt ctctacaaaa
    13741 gactttatta tatcaggaac atcagcaaaa taaacaggaa aaacaattac caatatatct
    13801 tcatttatgt ctatattatt ttcttttcta tgtatagcta aaggtataac ttctttactt
    13861 ccatttaacg tttcttttaa aatctttgaa actactagcg aatttcctgt tccagaaaaa
    13921 taataaatct tactattcat tcttatccct ctttctatat ttctacatca tcaatagtta
    13981 aaaagtctct taaaatagga tttatattta ctttccaatt ctatccaaaa tatcagtaaa
    14041 caaaggcaat tcttcatcag agatagattt aaatgtctct gttgcatata tacttgcaat
    14101 ttcatttaat ggttcttcta attctctacc taaatctgtt aggaatattt gaaacttacg
    14161 tctatcttta acatctactt tacggacaat taatccctta tttttcataa cgtctaaaat
    14221 tcttgttgta ttagatttat ccttacatga tttttcacat aaatctatct gagaaattcc
    14281 atcttgctcc catagaaacc ttaataacgc ataatggtca gttgttatat catatccatt
    14341 ttgtttaaac atctttttta aatcatcttt tacttttaac gaagtactta ctaacttagt
    14401 tgtagcttca tatagtttct caaaatcttt cattttacct ccatttaaat aatcccctgc
    14461 tcctcccatc tatcaattat tcccttatat tgctcaatat ctttaaaagc cattggcttt
    14521 aaaacagcat ttatagcaat aaaattatag atttgagaaa gcacaggtgg atttttcttt
    14581 tcctctatgc aataaataaa ttttttagat accttttgta tctttgattc tactttttca
    14641 gttataggat tttgattaaa tccagttttt atagataaac taccacaaac ataacctccc
    14701 gaatctaaga ccatggattt tataaaagaa attgcttttg aaccacgtgc attttcatat
    14761 gtagtaactg ctatacatgg cttacgatac aataatcttt caagcatcat atgtgctcta
    14821 tctgtaaaca ccttgaaaag tccagttaca ctacttccat aagttgggct accaaatata
    14881 acaccatcac tcttagtaat tatatcatga atatactcta ctttatcatt ttgattgata
    14941 cacttgccca ttttataaca atttaaacaa ccaatacaat atgatggatt tacctcactt
    15001 aaatcaaaat aattaatttt tacatctggt attaatcttt ctatatcttt atacaagtaa
    15061 gttaaaactt ttgatgtagc tccatttttt ctagggcttc catttataac tgtaattatc
    15121 atttctatat ctccttttta gttgttatat caacaataag tatatcaact atatttttag
    15181 ctgtcaatat aaaccattta tttaaagcta tacttacaca gcttaaataa aagttttatt
    15241 taactacatg tgcttattta atcaactata aatgaattat ttttaataat tgacaaacta
    15301 aattaatgat aatatacttt atgtattaca aatgtaagta gaaggaggta tagtatgaaa
    15361 aagttaccac atatttcaga agcagaatat caagttatga aaattatttg gaaatatgct
    15421 cctatcagca ctactgaagt aattgaaaag ttggtagaaa caagtacatg gagtcctaaa
    15481 acaattcaaa caatgttatt gagattagtt aaaaaaggag ctttaacata tgaaaaaaat
    15541 agcagagtat tcgtttatac tccacttgtt aaagaagaag aatacgtagc tacagaaagt
    15601 agttcttttt taaatcgttt ttataatggt actcttaatt ctatggtact aaacttttta
    15661 gaaaatgata aactatctga agatgatatt gaggaactga gagaaatctt aaataaaagg
    15721 acgactaagg gggataaata aatatgtatg ccccattctt tatacgattt ttactcagta
    15781 cattaacctt atcaatatta attgttataa ttttacttgc caaaaaagta tttaaaaaac
    15841 atatttctgc aaagtaccaa tacaatattg gatttatatt gcttataatg ttaataattc
    15901 catttgtccc tcttaaatat attaatcttg gaaatgtatt taactattta tatactttta
    15961 atcaacttgg aagcagtact aataggactt ctattataaa aaatagtcca aattctataa
    16021 ttaataactc aaatgcatta aacgattttt cagtatccct aaaccaagtt aattttgata
    16081 gtttaaatat tttattacta atatcatgga taattggaat tttaatcatg atagctatta
    16141 ctattcattg caatattaaa ataaaaaata tgaaaaaatc aatacaaatt ttaagagatg
    16201 aggagatagt atttatattt gaaaagtgta aaaaagatat tggtataaat aaggatttta
    16261 tactatgcaa atcacctatt ataaatacac ctattacatt tggatacttt aaaccacaca
    16321 ttatattacc agataagtct atatctaaat tatctttaaa agatataaaa tatatcttac
    16381 ttcatgaatt acaacacttt aaaaataaag atatactgat aaattatata atgtgcttcc
    16441 ttcaaatatt atattggttt aatcctcttg tatggtatgc atttaaagaa atgcgtatag
    16501 accgtgaaat tgcatgtgat atttcagttt taaaaaagct tgataaagat agtcatatag
    16561 aatatggaca aactctaata aactttgtgg ataatatttc aagaccatca aatcttacac
    16621 ttacatcaga tattggtggg tcaaaaaaac aaataaaaaa gcgactattg aagataatgg
    16681 attttcataa tgaatcaaat acgttaaaat tcaaaagcat tttaattttt acattgatag
    16741 ctttaattgt atttggaaat attcccaaac tatctattat ggcagatatc aacgaaaaat
    16801 atagacctac aaatgaacaa actgtgtacg aggacttaag tcagtatttt aaaggttttg
    16861 atggaagttt tgttatgtat gatttaaaat ccaagcaata tcatatttac aataaagaaa
    16921 aaagtaaaac aagaatttct ccaaattcta cttataagat ttatagtgcc ttattgggat
    16981 tggaaaatgg gataataaca agaaataatt cacatatccc ttgggatgga aagcatcatt
    17041 ctaatagttc ttggaatgaa gaccaagact tattttctgc tatggaaaac tccgtaaact
    17101 ggtactttca gactctagac aaaaaaacag ggctaaaaaa catacaaact tatcttaagc
    17161 aaattggata tggcaattat gatgtttcag gtggtatctc aaacttttgg ctagaatctt
    17221 cattaaaaat atcacctatt gaacaggtag aattacttga aagtttttat acaaataagt
    17281 ttaaatttga agataaaaat atagaaacag taaaagattc attgttaata tcaaaaaagg
    17341 atggggtatc actttttggt aaaacaggca ctgctaatat aaatggaaaa gatgtaaatg
    17401 gttggtttat aggttatgtg gaaaaaaatg aaaatactta tttctttgct acaaatattc
    17461 aaagcaaaga ttattctaat ggtagttcag catctaagat aacattatcc atattaaatg
    17521 acaaaaaaat ctataatcct taatatattt actgtacatt ctaaaaaatt cacataattt
    17581 tcatattaat tatcgattaa attcatatac aaggcttatt atataaacat agaagatagt
    17641 aaaactagct taaaaacata atataatacc ctatacaaat agtggcaact ctccctcaca
    17701 tcctcaattg ttgccactat ttaatttttt gtatgaagtt aaatatattt tcatcatttc
    17761 aacaatttgg gtgcagtaca atctctattt tgagacaacc tctttaattg ttttagagtt
    17821 tatgaattat ttctcatact ttaaaaagat tgaattactc ttgataatct tcttttgtag
    17881 tcattacata tcacctacta ctcaacacat attctttgac ctatttttac atccttgttt
    17941 atatccaaaa tatttacctt cttaccatct ggaaacaata ccattgtcaa agtagacttt
    18001 cctaattcat gtattctctt taaatcaaca ttaacaagaa catctccctg tttaaccata
    18061 tctccttgtt taacagcaat atcaaagcca tctccattta atgcaactgt atcaattcca
    18121 acatgaatta atacttctcc attagttcct ttaatcccaa atgcatgtcc acttgaaaat
    18181 actgtttcta atttacctga aataggagaa acaatttttc catctataat atcaatagca
    18241 aaaccatcac ctaacatttt tttagaaaat gtttcatctg gtacattttc taatgcaaca
    18301 attttcccct tagctggaga cacaaagttt tcattatttt cattaaacac taattcttcg
    18361 tcttcatctt catttatagc ttctttaggc actccaaata aacatgttac aataaagcag
    18421 aatgcaacca caaatattga tactaataag tatgtgatta aattatatcc atcatagata
    18481 tacattaaat aagaaggtaa aactgctaat ccatatgaat tagattgaat atgtaaaatt
    18541 gataatattg ctgctcctaa acctgaagat gcacacataa taatcaaagg ctttaaatta
    18601 tatcgaagct gaatcccaaa taaaacaggc tcacttatac caaaaagttg agatagcatt
    18661 gctccaactg cagtttgttt aacttgatta tttttagatt ttttcataaa tgctagacat
    18721 gttccaatat tcgcaaatcc atacattgca caaagagtaa taagtgcatt aaatcctgta
    18781 tttgctaata gtgatgtttc aatcattaca taagtatgat gtaagccagt aataacagct
    18841 aatggatatg taaatccaat aataaatcct ccaattccta aaggtaaacc aatcaataaa
    18901 ctgatgatat caaccatttt taattctatt gtatgcatta taggccctac acctaacaaa
    18961 ataactaaca ttgttattag cataacaaag aatggtgtga aaataaggtc taatacatta
    19021 ggcatatgtt tacggaacca tttttcaaga ttagctccaa taaatgctgt aacaatagct
    19081 gttaaaacac ttccttgaca gcctacaata ggaataaatc caaatgccat aattgcttca
    19141 acttcatttg atgggtcagc aacagaatat gcattaggaa gtattggaga aactaacata
    19201 agtccaataa caagtccaat aacaggtgtt cctccaaata ccttaaatgc tgaccataca
    19261 ataattgctg gtaaaaaagc aaatgctgtt tctgtaagaa ccgaaacaag tgtctgaata
    19321 tacaatggaa tatttgcaga agacattcca aataatccaa gtacattatc attaaacaaa
    19381 caacccttta atcccaagaa taatcctgtt gccgcaataa ctggaataat aggaacaaat
    19441 atatctgcta gagttctcat aactttctta aatccagttt cattattttt aactaattca
    19501 tcttgttctt tttttgataa agttttcaat cccatttttt caacttctgc ataaacttta
    19561 ttgactattc ccgttcctag aataatttga tactgtccag aagtaaagaa aacaccttta
    19621 acctcattaa ccttttccac tttcttatca ttaataattt ctctatcttt aaccatcacc
    19681 cttaatcttg tggcacaatg tgtaatagaa ataatattat tttcaccaat aatttcaata
    19741 agctcatttg ctattttatt atatttattc atttttctac ctcactttca ataatactgc
    19801 ttgaaatggt ttcaaactca ctgttttatt tagtagattt atatcatcta aattttggaa
    19861 tacaatatca tatttttctt ctgtttttat ttctacaagt ttatcagaaa aattataata
    19921 acaatcaata actaccaaat ctaattgcct tctatacgca ataacattgt cactttctat
    19981 atcaataggt tctattgaac cataaatcaa acaatcagaa tacttactat cttgtctcaa
    20041 ctttatcatc tttttataaa attctaaaat actttctgaa ttgttaatct gactctgtgc
    20101 attaatatct ttataatttt caataacctt taaccatggt gttgcatctg aaaaacctgc
    20161 attttcttta tctgcccatg gaaatggcgt tcttgcatta tctcggcttc ttaaattcac
    20221 aaattctaac gcttcttctt ctgagtaacc ttcatctatt gaacgatagt attggtcaat
    20281 gctagataaa tcattaaaat catttatggt atctctttta aaatttgtca ttcctaattc
    20341 ctgtccctga taaataaatg gtgttcctct taaaaagaaa tacattgctc ccaacatttt
    20401 tactgcatca tcattccttt cgtattgcaa aagatacttt gttgtagctc ttggttggtc
    20461 atgattttca ataaaattag ctgcccatcc atgttcctga acatacattt ggctatctaa
    20521 aatgcatttt cttaaatctg gaattgtcca tgaaattctt ttaaaccact cactaccaga
    20581 agcaacatct aagtcagcat gtttaaagtc aaagaccatt gaaaagaaac catcttctcc
    20641 aataaaatca cctagttggt catagctaat acctgctgct tcagcaacag tcatacaatc
    20701 atatttttta aacgtatttt cttttaattc ataaagcatt ttttctattc caggctgatt
    20761 tctaactgtt tttgtacatt taacaagacc atcaacacca tcactttcta aactttgaaa
    20821 acttaaattc tttttgataa atgtaatagc atcaactcta aatcctgcca ctcccatttg
    20881 agaccaggta ttaatcattt ggtaaagttc acttcttaat attggatttt cccaattaag
    20941 gtcaggttgt tttttaccaa aagaatgaaa ataatattcg tctctattct ctaccttttc
    21001 ccaaacactt ccaccaaaaa cagacctcca gttatttggt aaatcgtttc cttttttaaa
    21061 aatataataa tcatgataag gactttctgg attttttaat gcttcttgaa accaaggatg
    21121 ttcatcactt gtatgattaa gaactaaatc taatataatt ttgattcctc tatctttact
    21181 ttctttaatt aactctttta aatcttccat acttccaaat tcacttgcaa tatctaaata
    21241 atcactaata tcatacccat tatcgtccat aggtgattta aaaattggac ataaccacaa
    21301 tgatgtaata cctaaatctt ttaaataatc taaacgagaa ataattccct gtaaatctcc
    21361 aattccatca ttattactat cttgaaagct tcttggataa atttgataaa cgatttcttt
    21421 ttgccaccat ttcttttcca taaattactc ctttttatat gatatttctt ctttttaatt
    21481 ctttataaat tccattatcg aatatgtcct cacatataga tgtcgctata tctttaagtt
    21541 gttgatgact atttttcatt gcaattgtaa cgtctgatgc ttgaaacatg acaatatcat
    21601 tttgaccatc accaaaacat atggtttctt tttgagttac ccctaatctt tcttgtaatc
    21661 tcttaattgc tttgccctta tgaaaatctt tttgaataat ttcataatac tgactagaaa
    21721 tatctctctg tgctaattcc attttatctt gtagtatatc tttcacttcg tcaaatacct
    21781 tttcatttga ccataaacaa attttatgta catcttgtga cttatactct tcaatattat
    21841 tttcatatgt tattttttct tggatatgtt gtttatttat acaagaatta gttcctttca
    21901 actgattcat tgtttcaaat atttctttgg ctttttgatt catgaaaact ttttcctgac
    21961 tctcaatcga aaaagcaact tctctctttt ttaacaaaca aacaacttcc tttatcaatc
    22021 tttgattaaa agattgatta taaagtaatt caccatgata ttgaatgtaa ttacctcctc
    22081 ctgcaatata tccatcaacc cctaatgata aaacatcatc ttgtattgta cctatacttc
    22141 ttccagtaca aataacaaca ctgcaatgat tcttttgaca taaacgtata gcgtgctttg
    22201 cagattctgg gattccatat acctcatccc taagcgtacc atctatatca agtattaata
    22261 atttgtacaa tttcatcttc ctttcttact atcaaaatca taatagcatt caaaaaaaaa
    22321 gatatcaata ttttgatatc ttttaaaaaa tatgttcatt tttagcacaa attttcactt
    22381 tcggggaata tgttcacttt ctaaatacat cttgtattta atataaagca gttcaattaa
    22441 aataaaataa ggtgttgtta catcgtagtc cagttgttta tctacagaaa tagatataga
    22501 ttgaatatac aggttttcat cacataatct tgctaaagga ttacttatca tttttgtaat
    22561 agaaaccata taagttcctt tcattctcat ttctttagca aaatcaataa ttgtagatgt
    22621 atctccattc aatgagataa agaaaatcat atcatctgtt tttataaaat gattaagcgc
    22681 ctgaatcata tcataaccat aaacatcata tataattttc cctgttggta agaaaatcct
    22741 tttcatctct ttaacaactc tttcctgaga ataaccttct ccaaagacga ttatacgatt
    22801 tgcctttttc attctttcaa acataatcga acaatccttc ttctctatat catctatcac
    22861 cttattataa caatccatta attggtgttt actttgattt ttatattcta cttctgtttc
    22921 atttaagcgt ataataactc ttaattcact atatccaggt aattgtagtt tttgagcaaa
    22981 acgagaaagt gcacttgtag atatatggta tttgtctgca aattcatcta tagaaagttt
    23041 aatacaatct tctttatgat taagaataca ctcacagata tacttatcat ttgctgtaaa
    23101 tgaattataa tatttattga aaagtccttc aattttcatt cttttctcct tctatactct
    23161 atgtaattca tatgaagcaa atctaataga gcataaaatg ctcctacgac ttcataagat
    23221 atagcttgta atgtatcaag tttttgagtt tcaacataca gattatactc acaaagtgtt
    23281 gcaattgtat tattatcaag cctagttaaa gaaatcgtat gtatatgatt gttgataaat
    23341 tttgcaattt caattcctga ttttgtttct ccagacaaag aaatgataat aaataaatca
    23401 cttttttgaa atgattctct cataaattca atttcaccta tatcaaataa gtcaataaca
    23461 catttaccta ctgaaagaaa tattctcttt atttcttgag ctaaactttt ctgttcatta
    23521 cctgtcccat aaatataaat tgtttctgca tcatatatta ttttacaaat atcttgaaat
    23581 gatatttttt gtaattcatt cattaatagt cgataattct cgcaaacatc atgaaaatta
    23641 ttatcattat taacaaattc tagttgatta tttttaagta aatactttaa atcagagaat
    23701 gaatctaatc caatttttct acaaagcctt aataaagtag ctctagaaac attacattca
    23761 tttgcaattt tttcacacga aaaattacgg tattctcttt gacaaatcaa tatttgttta
    23821 ataataattt tatcattttc atttaaacta ttataacttt ggttaactaa cgattctaat
    23881 ctcatttttt ccactcccta aatattattt taatatacat tagttgcttt ttctagtgaa
    23941 gagtcttaaa taataaggat aaaataatca tttgatttta tttttattaa aataaaaata
    24001 acctgctaaa agcaggcacc aaaaagtact tatcctttcg aacatgtact ttagatattg
    24061 ctttcttcgg aagcttcatc tcttcatact ctaaaataga gttttttcta actcaaaagt
    24121 attttccatg gtagaaatta ctttaattca tctgccattt attttttatc ttatttaata
    24181 attactatta cttgaatatt tacttcttta agtaaatttc atagttaaca aagctaaaac
    24241 tattattata gatgttaaaa tacaaaatta taagtattgt tataaatatg cttacagcta
    24301 caaatataca tgatatatta aaaagaagct gtaaattatt atcatacata caagcaacta
    24361 tgcctctatt aatccatgtt aatttttatt atttaataca aggagttcca tgagacatac
    24421 atatccattc atattttaaa ttatccaatt ctcttataga tttcatcatc tttttataat
    24481 cccaattcca tggattagga taaggaacta tatttccatt cttatttttg actaaatcac
    24541 ctgcaaataa tacatcttca aaaatcatgc agacatgccc tggagtatga ccaggagtat
    24601 ggataatttt tatattccca atcttcatat tctctccata aggttcaaca tccttaatga
    24661 gccttctgga aattgccttt ccaataaact tcttaaaccc aggtctatcc ttctctccta
    24721 taataaaagg tatatcctct acatgagccc atacctttgc tcctgttttc tctttcaata
    24781 atttaatatt agatatatga tcaacatcat gatgagttaa taaaatatgc ttaatatctg
    24841 taagtttaat gccaagtgtt tttaattctt taattatatt tttccccata aaaggaagac
    24901 ctgtgtctat aagaataatt tctttatcga acacaataaa acaatttgaa ccacgagtac
    24961 tatctaacat taatacattt tcttttatct tcataaataa attcccctta tttggtattt
    25021 tacctatata attaattaat aaagttataa atagttataa tacttgatat taattttatt
    25081 ttttattaaa gtatactata ggtatacaaa tattatattt acataagtat accatcagta
    25141 tactttttta tcaatagaaa ggaataattt atatgtcaga attatcaaaa agagataaag
    25201 aaaaaataca aagagaaaat gagattattg ataaggcaga aaaattattt tgtcttaatg
    25261 gatttgatta aaaaatagct acgcacactc gtgactttag tcatgagtta gtagctaaaa
    25321 tagtcagcat atagggaaac ctatatgtag agataggata a
//
LOCUS       DAMJWP010000037        24939 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.038, whole genome shotgun sequence.
ACCESSION   DAMJWP010000037 DAMJWP010000000
VERSION     DAMJWP010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24939
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.038"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            4..1860
                     /locus_tag="PC700_001284"
     CDS             4..1860
                     /locus_tag="PC700_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDF2580600.1"
                     /translation="MDIHNKYVNFIKNIPVPFLYCRIVKRQEDIEYRVEYISKGMGKV
                     LQLEEGICDKNILDVLPVFKSKKYFKELFSNEVDCIKRYIPTLKNWINIKKQIIGDSY
                     IILYFGKIVFDYRQIIDSFDKKEKVAYIKDEEGIYIDCSENLIPILNNNIKTTKDIFG
                     KNDIEVWGENTGKLFRDDYRGGVSSKKRFLQNLFEYEETFFMVEKYFLYDEDELLGTI
                     GIIDNIIYSGYSNRNYNSKDLMKMIEHSIPENMFYKDVYGNYIGFNSGFLNLACMNKE
                     ELLGKNSYKISEEEALINKIFESDKGVVENKKVVTFELNVSINDENKCIEITKRPFFD
                     SYGSVIGIIGTARDISRRKRLEEEMDKTRMEFFANLSHELRTPINLISSSLQVIEKKE
                     ADLIESNDTLKRNLGIIKQNGNRILRLVNNVIDFTKMQSGYLDFKPEESDIIAFIEEI
                     CMSVADFASQNNIQLTFDTEIEEFSMLFDSEKLERIILNLLSNGIKYNKKDGKINIFL
                     YVKDNVFNMKISDSGIGIPKEKIDKIFNRFEQIDNELSYRVKGSGIGLSLVKSLVELH
                     EGSISLKSQLGIGSEFIVSLPVRSENNIEKYNHKREISNELSKKLEIEFSDL"
     misc_binding    1943..2183
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    2249..2484
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2569..3786
                     /locus_tag="PC700_001285"
     CDS             2569..3786
                     /locus_tag="PC700_001285"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HDF2580601.1"
                     /translation="MENKVKIGVLGYGVVGSGLIDIIDNNKEKRSIEIVGILVNNLEK
                     HKDKKYSNIITNNIDDIFNKDIDILVEVMGGLEPSLSYIKKALNNKIHVVTANKDLLA
                     ECGDELAKLASENKVSIKFEASVAGGIPVLKPIIESLEGNNIDSINAILNGTTNFILS
                     KMYDENLSYDMALRQAQELGFAEANPESDVLGYDAARKLSILSTLAYDNRVYWKDVYL
                     EGITDIDEKDIEYAKKLNCKIKLIGQSKYENDKVSAFVRPVLVEKDNILARIDNEFNA
                     VIVNGDSVGEVSFVGKGAGSLATGSAVYSDVIDIIDNRVSSIDSFTKDKIQVNKIVRE
                     KCGALLRFKKCNKDEILNIVENCLVKFDILNDDDELAIMVYADSEYEINNSLCLIKDK
                     GYCEKMNKMLKIS"
     gene            3900..4394
                     /gene="cdeM"
                     /locus_tag="PC700_001286"
     CDS             3900..4394
                     /gene="cdeM"
                     /locus_tag="PC700_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeM"
                     /protein_id="HDF2580602.1"
                     /translation="MENKKYANGGYSEDWYERGESTAKWFQNDREEYERAAYDEDRER
                     RGSNCGCSDSGGNRPRNCERFRREAEIREREAREAFCESSERKKEALAYECEARKLWE
                     EAEKYWDEYSKYNYKGIEYLAEAARLFDEGMECEARRNGNNGGNNNNCCHKCHKCNCN
                     CCRK"
     gene            4659..5924
                     /gene="hisD"
                     /locus_tag="PC700_001287"
     CDS             4659..5924
                     /gene="hisD"
                     /locus_tag="PC700_001287"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HDF2580603.1"
                     /translation="MKSIKTSSMKEKVDEKILREQVSSIIEDIRNNKDVALKKYNEKF
                     DRNTRDEFRITKEEIKEAYKHVDDEFINNLKIAAKNIREFAKAQKLSFENPFEKEIYP
                     GVILGQTNIPIESCLAYVPGGQYPLFSTALMLIIPAKVAGVKRIVACSPTMKNTEKIN
                     PKTLVAMDIAGADEIYATGGVQAIAAFTYGTEKINPVDIIVGPGNKFVTEAKRQCYGQ
                     VGIDFVAGPSEVLIIADETSNPVYIAADLLAQCEHDLNARGILLTNSLEVAQKVEKNI
                     ESMLKDLPTKDIAYSSWENNGEIILVDDMEEAIKISNFYAPEHLEVAVNEYNDICDRL
                     TNYGSLFIGNLSAEVFGDYVSGTNHTLPTLKASRYTGGVWVGTFIKTCTKQIFNEKAI
                     QSLAPVAEKLAKEEGLYAHAKAAEVRFKK"
     gene            complement(6127..6879)
                     /locus_tag="PC700_001288"
     CDS             complement(6127..6879)
                     /locus_tag="PC700_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2580604.1"
                     /translation="MSYFQYQGNSCFYKEYGQGKPIIFLHGNTGSSNMFKALVPLYVE
                     NFRCILIDFLGNGQSDRVSQFSPDIWHDEALQTIALIEHLNCGKVGLIGTSGGAWAAV
                     NTGLERPDLVEAIIADSFDGRTLNDNFIDNLITGREKSKQDIQARKFYEWCHGKDWEN
                     VVNLDTKALLQCANKKRPLFHKELCKLEMPILFTGSKEDEMCRHNLEEEYKQMATFIS
                     KASIHIFSQGGHPAILTNADEFAQLAKVFFKY"
     gene            complement(6997..7266)
                     /locus_tag="PC700_001289"
     CDS             complement(6997..7266)
                     /locus_tag="PC700_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580605.1"
                     /translation="MSYQNSIDLLPKELIEQVQEYIDGKVIYIPKKQEHKKHWGENTN
                     TKQVLASRNSQICINFQNGMSIKQLSEKYFLTEKSIQRILKQQNL"
     gene            7741..8835
                     /locus_tag="PC700_001290"
     CDS             7741..8835
                     /locus_tag="PC700_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDF2580606.1"
                     /translation="MKNIVGKPQTIKKVNEDLIKNIIKNEGPITKPEISSITELSLAT
                     VNKIVEQLALKNEVKVSGLSESTGGRRAQLFEINANLEHIIALYYYRNCCIGVVANLL
                     GEIIYQEEFNIRMDTYENVNQDTFFVIDTLIKKEKGNNIRAIGIGVPGVVKKGVVKNI
                     PTIKSWEGINLENLIKSKYNTKVFIENDTNITTIGIYQKQYKDKYKNMALMYLEDGIG
                     SGIVINGELYLGSTNFAGELSYLNIEFGKYNSNNLNLEDYIMSLRKRYLESKDNKFKK
                     EILSIISNFIRTLVCVLNPQAMIIQCSILTKSDMEFIEKDVSNNVGEENFPKLIKVES
                     LRERSINGVISMCLKETDYQYSLSNKKGGY"
     gene            8837..9787
                     /locus_tag="PC700_001291"
     CDS             8837..9787
                     /locus_tag="PC700_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HDF2580607.1"
                     /translation="MKYLVSIDGGGTKTKFCVSDLDGNILKEHTTGSTNYKSVGIKKT
                     YENINNGLKKILKDLYIDYDDIEYTVFGISGCDSPNDYKIIMDEILKIGINKEKIYLA
                     NDAVLAFYAQADSPGLVIVAGTGSIILGIKEDGEIYRVGGWGYNFSDLGSGYDIGRKL
                     LKKVLLYCDECHEYSDLFSCVLDFFGANSFEQLAYMITDINNNVEIANLASLVIDCAE
                     QGDKLAIEILRESSTELSKLAQVILRKISNPMKIDKSEINIVLSGGTLNKTVYAQMLM
                     NNLKTENTDKNLKFISQENQPVYGGIRLAMALANRRVKHG"
     gene            9780..10964
                     /locus_tag="PC700_001292"
     CDS             9780..10964
                     /locus_tag="PC700_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580608.1"
                     /translation="MDRKKVIVQIFTGGFKAKEIKFNDIKCKLLELLDCIDIEKVIMG
                     WSVDEELYVETKKLLNKYNVELYLWLPCFSEIGLLEKSNLLIDYMGKEVKGYALQEDE
                     NFEFYCPNNKENIKNIKKIYSTYFSDIKFDGIFLDKIRYGSFSNKLNGVFNCFCKDCL
                     NIYEKNNIDKKSLMREMNNVSKGVIDNEDTPSGITEYINGKYKFKNKIWEDFFETKNQ
                     NIYKSLLEISSYFKSKGLKIGIDTFSPFISYFTGQDLKKLQEVADFIKPMMYRTTKAP
                     AGLPFEFERFIDESIHKNKEIGKRNFLDILGIKEYEGDVYPIEFVLEEIDFMTRNLNK
                     DIYVGVEINKKEIIAPVTPKYISENISNINKTTVKGYVLSWDLLSAPKENIDEIIKWF
                     RN"
     gene            10977..12473
                     /locus_tag="PC700_001293"
     CDS             10977..12473
                     /locus_tag="PC700_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDF2580609.1"
                     /translation="MSKILEMQQIYKSFGKVDVLKGINFELEKGEVRALLGANGAGKS
                     TLIKILGGVHSQTKGKVILTGNEIMFKNSHHSKENGISIIYQELSLIPTLTVLENIFL
                     GREILKGSVFLNKKDMLEEYMKICEDFDFNIDPHTVVSNLSVANQQMVEIMKAVSCNS
                     EIIIMDEPTTSLTNNEKDGLFKIISRLKSKGKTIVYISHILEEVFKVCDSASIMKNGI
                     MIGTYDVEDLTKLKITQLMTGNDNHKLIEKKDYTFAKYEEPPVLEVKNISWSNKIKNV
                     SFKVYRGEVVGLAGLVGSRRTEIINLIFGIDNKDSGDIFINGKKVDITSPKVAIKNKI
                     GLIPEDRKNLGLILEQAIYTNSSSVQIDKFKKFLFIDRNKEIEFSKKGVKELGIKVSD
                     VKQEVKELSGGNQQKVVVSKWLEKDLDLIIYDEPTKGIDIAAKEDIFNTIKEFSSEGI
                     GVIFISSDLEEVIRVSDRILVTREGSIVDDLKNENIQVQDIMNSIFNV"
     gene            12496..13446
                     /locus_tag="PC700_001294"
     CDS             12496..13446
                     /locus_tag="PC700_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2580610.1"
                     /translation="MKKAFEKLNLRDYGVLLGFLALCIAISIATPSFLGKQNILNLLR
                     QSSIIGIISAGMTFVIISGNFDISVGAVAALAGAITMKFATGGTNLFASMLLGVAICA
                     VIGLINGIVVAKINVPSLIATMAMVTIVRGLLLMLTGGYPITENIPILDYIGNGYLFN
                     IPIPVVIFFIVVLVSFIVLNKTKFGRYVYSVGGNQEASKLNGINVDSHKVKVFIINAV
                     LAGIAGIVLTGRLGTATAIAGEGYDMDAIASVVIGGTSVAGGSGSVLKTVIGVLLMSV
                     INNSFNLLGIDVYFQYIFKGLIILAAVGFDSYSKKKLASR"
     gene            13467..14429
                     /locus_tag="PC700_001295"
     CDS             13467..14429
                     /locus_tag="PC700_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDF2580611.1"
                     /translation="MKKLMKHLALLLSLVMIFGLVGCSNGGDKEKNGEKKIAVLLPGS
                     TGYFVATKQGIDAKSKELGVSVEYADAQWDASKQLSQAEDFMAKGVDMIALCGVDSAV
                     SERIVKAANDSDVPIVAFTNSIGSNPTGEFKGLVTYIGQNEEETGALTGKIAKNLLGE
                     TGGKAVLIEGVPGTTPQVLRKKGLEKELKDSNIEIVYNQTSNWEKEQALKVTEDLIQK
                     KTDFNVIICQDDNSATGAGQALKDAGLKDKVKVIGLGGSKDGLKAITDGLIDGTTYMS
                     AVEEGGLVIEKASKFLKGEKIEPVTQIKQVEVNKDNISEFKGEW"
     gene            14801..15433
                     /locus_tag="PC700_001296"
     CDS             14801..15433
                     /locus_tag="PC700_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2580612.1"
                     /translation="MARRLFLERIGLIFLGSAILAFGVYNFYYLNNITEGGVLGILLL
                     LKNLFNIQPAIANVVIDGLLLLVGYKFFGKKFLIYSIVASITFSVLYDLFEAIGPLVP
                     QSQNMLLSTILAGVTVGTGVGIVVKAGCASGGDDALALVISKTTSLNIGQVYLATDVI
                     VLLLSLFYLSAFDIFYSLIAVTISGKVIDFIYYHGKSLDMDISNDIVPEC"
     gene            15844..15936
                     /gene="kdpF"
                     /locus_tag="PC700_001297"
     CDS             15844..15936
                     /gene="kdpF"
                     /locus_tag="PC700_001297"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HDF2580613.1"
                     /translation="MEGLPMWFLAVVIIMLIIYLVYALINPEKF"
     gene            15977..17659
                     /gene="kdpA"
                     /locus_tag="PC700_001298"
     CDS             15977..17659
                     /gene="kdpA"
                     /locus_tag="PC700_001298"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HDF2580614.1"
                     /translation="MFMVQIVIVLAIFMILVIPMGKYLYHIATNQKTFGDRLFDKVDN
                     FIYKVCSIDKKKEMNWKQYALALLFTNAVMVFIGYIILRTQSMHIFNPSGIKSMEQGL
                     SFNTIISFMTNTNLQHYSGESGLSYFSQMTVIIYMMFTSAATGYAAAMAFVRGLVGKK
                     KTLGNFYVDLIRITTRVLLPGALIIGLILVTQGVPQTFAGTETVTTIEGKLQDIARGP
                     VAALESIKHLGTNGGGFFGSNSSHPFENPTIISNIVEILSMMILPGACVVAFGHMIKN
                     KKQGWVVFGAMSIIFLIGLVVCFKAESAGNPILSQLGLNQSMGSMEGKEVRFGIAQSS
                     LFTTVTTSFTTGTVNNMHDTLTPLGGLVPLLNMMLNVVFGGKGVGLMNMLMYAIIAVF
                     LCGLMVGRTPEFLTKKIEGKEMKLIALLIILHPLLILMFSGLSVAIPAGLEGISNPGF
                     HGLSQVLYEFASSAANNGSGFEGLGDNTMFWNITTGIVMFFGRYVSIIVLLAISSLLA
                     SKKAVNESIGTLRTDNFTFTIVLVLVVLIVGALTFFPALALGPISEHLVLWH"
     gene            17684..19741
                     /gene="kdpB"
                     /locus_tag="PC700_001299"
     CDS             17684..19741
                     /gene="kdpB"
                     /locus_tag="PC700_001299"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HDF2580615.1"
                     /translation="METKKTKFITKDIFKTSIIEAFKKLNPKYMMKNPVMFVVEIGFI
                     VTLILTIVPSLFGDEGNNLRAYNGIVTIILFITVLFANFAEAVAEGRGKAQADTLKKT
                     RKDTVARVIDTLGNEKMISASELKLGDIVLVNVGEVIPNDGEVIEGIASVDESAITGE
                     SAPVMRESGGDFASVTGGTTVVSDWLKIRITTKPGESFLDKMIALVEGASRQKTPNEI
                     ALNTLLVGLTLIFMVVLISLYPMANYAGVKIPISTMIALLVCLIPTTIGGLLSAIGIA
                     GMDRVTRFNVIAMSGKAVEACGDVDTMILDKTGTITYGNRLASEFIAVGNADSKDLIN
                     YSVMCSLKDDTPEGKSIVDLGMKLGSTGKTKEAEEAEFVEFSAQTRMSGINLADGRAI
                     RKGAYDSIIKRVKESGGTVPEDLEENVNRVAKLGGTPLVVCVDNEIYGIIYLKDTVKS
                     GLVERFARLREIGIKTIMCTGDNPLTAATIAREAGVDGFIAECKPEDKIDAIKKEQLE
                     GKIVAMTGDGTNDAPALAQADVGLAMNSGTTSAKEAANMVDLDSDPTKILEVVEIGKQ
                     LLITRGALTTFSIANDVAKYFAIIPAIFTLAIPQMEILNVMKLSTSFSAILSALIFNA
                     IIIPCLIPLAMKGVKYRPMKSQAMLMRNMLIFGLGGVIVPFLGIKLIDLLITPLVMIL
                     NLG"
     gene            19760..20386
                     /locus_tag="PC700_001300"
     CDS             19760..20386
                     /locus_tag="PC700_001300"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HDF2580616.1"
                     /translation="MKTLKPALLVSIVLLVVCGLVYPLVLTGVSQVAFKDKANGSMIE
                     VNGVKVGSELIGQSFTDARFFKGRVSSVNYNTYTKEDLVPDKDGNASYGGVSSGSFNY
                     GATNPELHDRVQKDIEKFLKDNPTVKREDIPTDLLTASGSGLDPNISPASAKIQIPAI
                     AKASGISESKLQKIVDDNTSKKLFGVLGEDRVNVLKVNIEVAKILGLI"
     gene            20623..21531
                     /gene="cysK"
                     /locus_tag="PC700_001301"
     CDS             20623..21531
                     /gene="cysK"
                     /locus_tag="PC700_001301"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDF2580617.1"
                     /translation="MLYNNALELIGNTPVVRLNNLGCTNGCSNIYVKLEKVNPAGSIK
                     DRAVYEMLEGLEQRGELKKGDVLVEATSGNTGIALSMIGKLKGYEVVIVMPETMSVER
                     RDLMKAYGANLVLTDGKAGMAGSIEKANELLKENPNYKSLKQFENKDNPNAHYKTTAV
                     EIMNDVKDLDIFVCGVGTGGTLIGTARYLKEQNPNIRVIALEPKKSPAISENKTGPHK
                     IQGIGANFVPENYDSSVVDEVILVDDEDAFDTVKLLAAKEGILVGISSGANIFGAIEM
                     AKKYPDKKIVTVAPDGVDKYMSMGIF"
     gene            21558..22148
                     /gene="cysE"
                     /locus_tag="PC700_001302"
     CDS             21558..22148
                     /gene="cysE"
                     /locus_tag="PC700_001302"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDF2580618.1"
                     /translation="MFKKINKDIEYIMKNDPAARSKIEVFLLYPSVHAMIMHRMAHAL
                     YKKKKLFTARLISQISRFMTGIEIHPGAKMGEGILIDHGMGVVIGETAEVGNRVTIYQ
                     GATLGATGKDTGKRHPTVGDDVLIGAGTKILGPLNIGSNSKIGANSVVVKDVPNGATV
                     VGIPAKIVKIRNLEPVKKNKKEVSYEYDELDNVYYI"
     gene            22308..22496
                     /locus_tag="PC700_001303"
     CDS             22308..22496
                     /locus_tag="PC700_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDF2580619.1"
                     /translation="MKANVNQDTCIGCGLCPSICPEVFDMKDDGKSHVIVDEVPSDAE
                     ESAAEARESCPVDAIDVH"
     gene            complement(22550..23419)
                     /locus_tag="PC700_001304"
     CDS             complement(22550..23419)
                     /locus_tag="PC700_001304"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HDF2580620.1"
                     /translation="MKLNRNDSCWCGSTKKYKKCHMDMDNKIDSFAKLGHIVPTHDII
                     KNESQIEEIKKSAVINTKILDYISDKIEIGMSTEDMNKLVHDFTLQNGAIPAPLDYNG
                     FPKSVCTSVNNEVCHGIPSKDIILKDGDIINVDVSTIFNGYYSDASRMFMIGNVNENA
                     KKLVQVTKECVQKGLEVAKPWGFLGDIGDAINSYAKKNGYSVVREIGGHGVGLSFHED
                     PFVSYVSKKGTEMLLVPGMIFTIEPMVNEGTHEIFVDEENNWTIYTKDGKLSAQWECM
                     VLVTETGVEILTE"
     gene            complement(23616..24860)
                     /locus_tag="PC700_001305"
     CDS             complement(23616..24860)
                     /locus_tag="PC700_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580621.1"
                     /translation="MENNLNIAKKNNTELLQNDSLKVNLQIGVDFTLLSKSLKAFYKK
                     EKDFYCIALAPCNVSSEKANRKISIYDMIKEINILIESITDKNELLSEELFTNNLSEF
                     YDESLDEIYIDLKQAYLYIKKNTDSLNIEDSIEYVFDVDISNDIKAKENPLINFNSLS
                     LVVWSGETKHILQQMNILDLEDIHSYYMENNTNITIYENNTLQYKFDEESEDIKDSSI
                     DIIEQMLILPDDLNYDKDEVENMKSRLAKINIKYLQTLKEKDIKIKLINSNLTDEPEF
                     SDLKYQLPPCWVRSGKTWKDVPGIYRNNSIVAKIGYSNPSYANVHSSKNLELHETAHA
                     IDKNVLNKKSNSEEFMEVFAQERYKLYDPKQVAHAYISKFIEEFFAESFVHYYLDEDS
                     KNTLKENCPLTYDFLEKLELNY"
BASE COUNT         9595 a         2678 c         4496 g         8170 t
ORIGIN      
        1 aatatggata tccataataa atatgtaaat tttataaaaa atatacctgt acctttttta
       61 tattgtagaa ttgtaaagag acaagaagat atagaatatc gagtagaata tataagtaag
      121 ggtatgggca aagtattaca attagaagaa ggtatttgtg ataaaaatat actagatgta
      181 ttgcctgtat ttaagtctaa aaaatatttc aaagagttgt tctcaaatga agtggattgt
      241 ataaaaaggt acattccgac acttaaaaac tggataaata taaaaaaaca gataataggg
      301 gattcataca taattttgta ttttggtaaa attgtatttg attatagaca gataatcgat
      361 tcctttgata aaaaggaaaa agtagcttat attaaagacg aagaaggcat atatatagat
      421 tgcagtgaaa atttaatacc aatattaaat aacaacatta aaacaacgaa agatatcttt
      481 ggtaaaaatg atatagaagt atggggagag aatacaggaa aattatttag agatgattat
      541 agaggaggag tatctagtaa aaaaagattt ttacaaaatc tttttgaata tgaagaaaca
      601 ttttttatgg ttgaaaaata ttttttgtat gatgaagacg aacttttagg tactataggg
      661 ataatagata atataatata tagtggttac agtaatagaa attataactc aaaagattta
      721 atgaagatga tagaacactc aattccagag aatatgtttt ataaagatgt gtatggaaat
      781 tatattggat ttaattcagg ttttttgaat ttagcttgta tgaataaaga agaattatta
      841 gggaagaata gttataaaat atcagaagaa gaagctttaa taaataagat atttgaaagt
      901 gataagggtg tagttgaaaa taaaaaagta gttacatttg agttaaatgt aagtataaat
      961 gatgaaaata agtgtattga gattacaaaa aggccgtttt ttgatagcta tggaagtgtt
     1021 attggaataa ttggaacagc aagggatata agtagaagaa aaagattaga agaggaaatg
     1081 gataaaacta ggatggaatt ttttgcaaat ttatcccatg aacttagaac gcctataaat
     1141 ttaatatcat cttcattaca agtgatagaa aaaaaagaag cagatttaat agaatcaaat
     1201 gatactttaa aaagaaattt aggtataata aaacaaaatg gaaatagaat actgaggtta
     1261 gtaaataatg ttatagactt tacaaaaatg caatcaggat atttagattt taaaccagaa
     1321 gaatctgata taattgcatt tatagaggag atatgtatgt ctgtagctga ttttgcaagc
     1381 caaaataata tacaacttac atttgataca gaaatagaag aattttcaat gttatttgat
     1441 tcagaaaaat tggaaagaat aatattaaat ttactttcta atggaattaa gtacaataag
     1501 aaagatggaa aaataaatat atttttatat gtgaaagata atgtatttaa tatgaaaata
     1561 tctgatagtg gaataggtat accaaaagaa aaaatagata aaatatttaa tagatttgaa
     1621 cagattgaca atgaactttc ctacagagtt aaaggaagtg gtattggact ttcattagtg
     1681 aaatctttgg ttgaattgca tgagggaagt atatctttaa aaagtcaact tggtattgga
     1741 agtgaattta tagtttcatt acctgttaga agtgaaaata atattgaaaa atataatcat
     1801 aaaagagaaa tcagcaatga actaagtaaa aaattagaaa tagagttttc ggatttataa
     1861 gtttaaatat aaaaaataaa attttaaaaa agtattgaca attgatttag aagggtttaa
     1921 aataaattac aacataataa ataaaaaggc gatgaatagg aagagtaaat ctaattcgat
     1981 taccagagag aagagtccat tggctggaag actttttaaa ttagagggat tgaaaactac
     2041 ctaggagcta ctttctgaag tcatctgcat tagtagattt tagtaagaat agtcgttagc
     2101 tgcgttaaag tgtaaagtga gttttcatga tatatgaaaa ctaaattagg tggaaccgcg
     2161 ggaataatac tctcgtcctt atgttataag gatgagagtt ttttgttgtt taaaaatata
     2221 aacagtaatc ataataatta tagaaaatat aaaagcgatg aataggaaga gtaaatttaa
     2281 ttcgactatc agagaggaaa gttcacaggc tgaaagactt tctaagtttg agaaattgaa
     2341 aactacctag gagctgtggt ctgaagttac tttaaaaagt tttagtaaga ctaaccgtta
     2401 actgcgttaa agtgtaaagt ggattttatc aataaatgat aaaatcaaat tgggtggaac
     2461 cgcgagtata gactcgtccc tttatatagg gatgagtcta tttttattgc aataaaagat
     2521 tgaaaataaa aaaaaatcgg aaaattataa atttaggagg aaaataaaat ggaaaataaa
     2581 gtgaaaattg gagtactagg atatggagtt gtaggaagtg gacttataga tataatagat
     2641 aataataaag aaaaaagaag tatagaaata gttggaattt tagttaataa tttggaaaaa
     2701 cataaggata aaaaatattc taatataata actaataata tagatgatat atttaataaa
     2761 gatatagata tacttgttga agtcatgggt ggtttagaac catcattatc ttatataaaa
     2821 aaagcattaa acaataaaat tcatgttgtt acagcaaata aagatttgtt agctgaatgt
     2881 ggagatgagc ttgcaaaatt agcaagtgaa aataaagtaa gtattaaatt tgaagcttca
     2941 gtcgcaggag gtattccagt attaaaacca ataatagaat cactagaagg aaataatata
     3001 gatagtataa atgcaatttt aaatggaact actaatttta tattatctaa aatgtatgat
     3061 gagaatctat cttatgatat ggcattaaga caagctcaag aattaggttt tgctgaagca
     3121 aatccagagt cagatgtatt agggtatgat gctgctagaa aactatcaat attatcaact
     3181 ctagcttatg acaatagagt atattggaag gatgtgtatt tagaaggaat tacagatata
     3241 gatgaaaaag atattgaata tgcaaaaaaa ttaaattgta aaataaaatt gattggtcaa
     3301 agtaagtatg aaaatgataa agtaagtgct tttgttagac ctgttttagt agaaaaagat
     3361 aatatccttg ctagaataga caatgagttc aatgctgtca tagtgaatgg agattcagta
     3421 ggagaagttt cttttgtagg taaaggagct ggaagtttag caacaggaag tgcggtttat
     3481 tcagatgtta tagatataat agataataga gtttcaagta tagattcttt tactaaagat
     3541 aaaatacaag ttaataaaat agttagagaa aagtgtggag cacttcttag atttaaaaaa
     3601 tgtaataaag atgaaattct aaatatagtt gagaattgtt tagtaaaatt cgatatttta
     3661 aatgatgatg atgaacttgc tattatggtt tatgctgatt ctgaatatga aataaacaac
     3721 agtttatgtc taataaaaga taaaggatat tgcgaaaaaa tgaataaaat gttgaaaata
     3781 agttaaaaaa gaaaaaataa gtttatatgt aacatatttt gacgatttta atactatata
     3841 gtatgagatg aaacaaaaca tctcataaaa aattaaaatt caagggagag atatgttata
     3901 tggaaaataa aaaatatgca aatggtggtt attcagaaga ctggtatgaa agaggagaat
     3961 ctacagctaa atggttccag aatgatagag aagaatatga aagagcagca tacgatgaag
     4021 atagagaaag aagaggctca aactgtggat gttcagattc aggaggaaat agacctagaa
     4081 actgtgaaag atttagaaga gaagctgaga taagagaaag agaagcaaga gaagcattct
     4141 gtgaatcttc agagagaaag aaagaagcac tagcatatga atgtgaagct agaaaattat
     4201 gggaagaagc agaaaaatac tgggatgaat attcaaaata caactataaa ggaatcgaat
     4261 atttagcaga agctgctaga ttatttgatg aaggtatgga atgtgaagct agaagaaatg
     4321 gaaataatgg aggaaacaat aataattgtt gccataaatg ccataaatgt aattgtaact
     4381 gctgtagaaa ataaaaaaat tcattgacaa atcaatagtt aaaatatatc atattatgta
     4441 taataaagag aaaattcaga aaaaaataat gattagaata aatttaagta tatttattag
     4501 acaaatagtg ctcagttagt gaggtgagta caatatgatt aaactcccat cctctagctt
     4561 ttgatagagg gtgggagttt ttatttattg atatatctta aatgaggata atattgtaaa
     4621 ataaatgagt atgagattgt aaaattagga ggtaaaaaat gaaaagtatt aaaacatcaa
     4681 gtatgaaaga aaaggtagat gaaaagatat taagagagca agtaagttct ataatagaag
     4741 atataagaaa taataaggat gttgcactta aaaaatataa tgagaagttt gatagaaata
     4801 caagagatga atttagaatt actaaggaag aaataaagga agcttataag catgtagatg
     4861 acgaatttat aaataactta aagattgctg ccaaaaatat aagagaattt gctaaagctc
     4921 aaaaactaag ttttgaaaac ccttttgaaa aagaaattta tccaggtgta atcttaggtc
     4981 aaactaatat tccaatagag tcatgtcttg catatgttcc aggaggtcag tatccattgt
     5041 tttcaacagc tcttatgctt ataatacctg caaaagtagc aggagtaaaa agaatagtgg
     5101 catgttcacc aactatgaaa aatactgaaa agataaatcc taaaacattg gtagctatgg
     5161 atatagcagg tgctgatgaa atatatgcaa ctggaggagt tcaagctata gcagcattta
     5221 catatggaac agaaaagatt aatccagttg acataattgt aggaccagga aataagtttg
     5281 taactgaagc taaaagacaa tgttatggac aagtaggaat tgattttgtc gctgggccaa
     5341 gtgaagttct tataatagcg gatgaaactt caaatccagt ttatattgca gctgatttat
     5401 tagcacaatg tgagcatgat ttaaatgcaa gaggaatatt attgacaaat agcttggaag
     5461 tagcacaaaa agttgaaaaa aatatagaaa gtatgctaaa agatttgcca acaaaggaca
     5521 tagcatatag ttcatgggaa aataatggag agataatatt ggttgatgat atggaagaag
     5581 ccattaaaat aagtaatttt tatgcaccag aacatttaga agttgcagta aatgagtata
     5641 atgatatttg tgatagatta actaactatg gttctttatt tattggaaat ctttctgctg
     5701 aggtgtttgg tgactatgtt tcaggtacca atcatacatt accaacactt aaagcatcaa
     5761 gatatactgg tggtgtttgg gttggaactt ttatcaaaac ttgtacaaag cagatattta
     5821 atgaaaaagc tatacaatca ttagcacctg tagctgaaaa gctggcaaaa gaagagggat
     5881 tatatgcaca tgcaaaggct gctgaggtta ggtttaaaaa gtagtaaaaa attttatttt
     5941 ggcagtcgag atttagtatt agactgagat aaagaaaacg ctgagaacct gaataaatac
     6001 tatattttgt gggcctcggc gtttctttta tatctgtata taataggata atactacctg
     6061 tgtgtttaaa tgctaactta atatttcttt tctaaataat gtttatgaaa tagttaatat
     6121 ttttagttaa tacttgaaaa aaacttttgc taactgtgca aattcatctg catttgtcaa
     6181 tatagcaggg tgtcctccct gactaaaaat gtgaatactt gcttttgaaa taaaagttgc
     6241 catttgcttg tactcttctt caagattatg acgacacatt tcgtcttcct tacttcctgt
     6301 aaataaaatt ggcatttcta atttacacaa ttctttatga aataaagggc gtttcttgtt
     6361 tgcacattgc aatagagctt ttgtatcaag attaacaaca ttctcccaat cttttccatg
     6421 acaccattca tagaattttc gtgcttgaat atcttgcttt gatttttccc ttccagtgat
     6481 tagattatct atgaagttat cattaagagt acgtccatca aagctatctg ctataattgc
     6541 ttcaactaaa tcgggacgtt ctaatccagt attaacagct gcccatgctc ctccacttgt
     6601 tccaattaag ccaactttac cacaattcaa atgttcaatc agtgctattg tctgtaaggc
     6661 ttcatcatgc catatgtcag gagaaaattg agaaactcta tcagactgtc cattacctaa
     6721 aaaatcaatt aagatacatc taaaattttc tacatataat ggcacaagtg ctttaaacat
     6781 attagaagaa ccagtattac catgcaggaa aattatcggt ttaccttgtc catattcttt
     6841 ataaaaacaa gaattacctt gatactgaaa atatgacata tattttcctc ctttttattt
     6901 gttaagttaa ttataacaat ataggtatga atgaaaaacc tcatgttctt tcttagaata
     6961 aaaatgtgcc acgcataaaa cgtgacacat ttctaattat aaattttgct gtttaagtat
     7021 tctttgtatg cttttctctg tcaaaaagta tttctctgat aactgcttga tactcattcc
     7081 attttggaaa tttatacaga tttgagaatt acgagaagct aagacttgtt tggtgtttgt
     7141 gttttcaccc caatgctttt tgtgctcttg ctttttggga atataaatca ctttaccatc
     7201 tatatattct tgcacttgct caatcagttc tttaggcagt aagtcaatcg aattttgata
     7261 gctcattttg cacctcctaa ataattactc tttaggatta gcaaaggcta ctgctactca
     7321 caattaaaaa taaaattttt tattatgtac aatagccttt gctatgcaca agttccaatt
     7381 cccataaact aaatactcct ttctggtttg aaaaattcat ctgaacattg atttcgattt
     7441 taagatagag ttcaatgaca aatttacatt tttattatat atattcagat agataattgc
     7501 aactgtcttt gtatgaggat attttattat agtcaatact ataatatata tttatatcat
     7561 caaactaata tgaacagttg aaaggaatat gaacaataaa acttagtcag gaagaataat
     7621 tatgaagcag acaattgaga aatttgattt taaagtttta ttgaaaattt attgacaaat
     7681 ttaacttgta aatattatac taaacttaat aaataaagtt tataaagtaa ggtgttgtct
     7741 atgaaaaata tagttggaaa accgcaaacg ataaaaaaag taaatgaaga tttaataaaa
     7801 aacattataa aaaatgaagg tcctataaca aaaccagaaa tttcaagcat tactgaattg
     7861 agtttagcta cagttaataa gattgtggag caattagctt tgaaaaatga agttaaagta
     7921 agtggactaa gtgaatctac tggaggaaga agagctcaat tatttgaaat aaatgcaaac
     7981 ttagaacaca taatagcttt atattattat agaaattgtt gtataggggt agttgccaat
     8041 ttattaggtg aaataattta tcaagaagaa tttaatataa gaatggatac atatgaaaac
     8101 gttaaccaag atactttttt tgttatagat actctaataa aaaaggaaaa aggaaacaat
     8161 ataagggcta ttggaattgg tgtcccagga gttgtaaaaa aaggtgtagt taaaaatata
     8221 ccaactataa aaagttggga aggtataaat ttagaaaatt taataaagag taagtacaat
     8281 actaaagtgt ttatagaaaa tgatacaaat attactacta ttgggattta tcaaaaacag
     8341 tataaagaca aatataaaaa tatggctctt atgtatttag aagatggtat tggttcaggt
     8401 atagttataa atggtgaatt gtatttaggt tctacaaatt ttgctggaga attaagttat
     8461 ctaaatatag aatttggtaa gtataactct aacaacttaa atcttgaaga ttatattatg
     8521 agtttaagaa aaagatattt agaaagtaaa gataataaat ttaagaaaga aattttatct
     8581 ataatatcaa actttataag gactcttgta tgtgtcctaa atccacaagc tatgattata
     8641 cagtgtagta tattaaccaa gagtgacatg gagtttatag aaaaagatgt aagtaataat
     8701 gttggagaag aaaattttcc taaattaata aaagtagagt cattgagaga aagaagtata
     8761 aatggagtaa ttagtatgtg tttaaaagaa actgactatc aatattcctt atctaataaa
     8821 aaaggaggat attaatgtga aatatttggt ttcaatagat ggagggggaa caaaaactaa
     8881 attttgtgta agtgacttag atggaaacat acttaaagaa cataccactg gttcaactaa
     8941 ttataagtca gttggaatta aaaaaacata tgaaaatatt aataatggac ttaaaaaaat
     9001 attaaaagat ttatatatcg attatgacga tattgagtac actgtatttg gtatatctgg
     9061 ttgtgattct cctaatgatt ataagattat aatggatgaa atattaaaaa ttgggattaa
     9121 caaagaaaaa atatatctgg caaatgatgc agttttagct ttttatgcac aagctgattc
     9181 ccctggttta gtaattgtgg ctgggacagg ttcaattata ctgggaatta aagaagatgg
     9241 agaaatatat agagttggtg gatgggggta taattttagt gatttgggtt ctggatatga
     9301 tatagggaga aaattattaa agaaagtatt gctttattgt gatgaatgtc atgagtatag
     9361 tgatttattt agttgtgtct tggatttctt tggagcaaat agttttgaac aacttgcata
     9421 tatgattact gatataaaca ataatgttga aatagcaaac ctagcttcat tagtgattga
     9481 ttgtgccgaa caaggtgata aattagctat tgaaatatta agagaatcat caactgaact
     9541 atctaaatta gctcaagtaa tattgagaaa aatatctaat ccaatgaaaa tagataaaag
     9601 cgaaataaat atagttttgt ctggtggtac attaaataaa actgtatatg ctcaaatgtt
     9661 aatgaataat ttaaaaacag aaaatacaga taaaaattta aaatttatat ctcaagaaaa
     9721 tcagccagta tatgggggga taagacttgc tatggcatta gctaatagga gagttaagca
     9781 tggatagaaa aaaggtaata gtacaaattt ttactggagg ttttaaggct aaagaaataa
     9841 agtttaatga tataaaatgt aaactactag aattattgga ctgtattgat atagaaaagg
     9901 ttatcatggg atggagtgta gatgaagaat tatatgtaga aactaaaaaa ctattaaata
     9961 aatataatgt tgaactatat ctttggttgc catgtttttc tgaaatagga ttacttgaaa
    10021 aaagtaattt actaattgat tatatgggga aggaagtcaa gggctatgct ttacaagagg
    10081 atgaaaattt tgaattttat tgcccaaata ataaggaaaa tataaaaaat ataaagaaaa
    10141 tatatagtac ttattttagt gatattaaat ttgatggaat ttttttagat aaaataaggt
    10201 atggttcttt ttcgaataaa ctaaatggtg tttttaattg tttttgtaaa gattgtttaa
    10261 atatatatga aaaaaataat attgataaaa agtcgttaat gagagaaatg aataatgtgt
    10321 ctaagggagt tatagataat gaagatactc catctggtat aacagaatac attaatggaa
    10381 aatacaaatt taaaaataaa atatgggaag atttttttga aacaaaaaat caaaatatat
    10441 acaagtcatt attagagata agtagttatt ttaaaagtaa gggattgaaa ataggtatag
    10501 atactttttc tccttttatt tcttacttta caggtcaaga tttaaaaaaa ttacaagaag
    10561 tagcagattt tataaaacca atgatgtata gaactacaaa agctccagct ggtttaccat
    10621 ttgaatttga aaggtttatt gatgaatcca tacataagaa taaagagatt ggaaaaagaa
    10681 atttcttaga tattttagga attaaagagt atgaaggaga tgtatatcct atagaatttg
    10741 tactagagga aatcgatttt atgacaagga atcttaataa agatatatat gtaggagttg
    10801 aaataaataa aaaggaaatt atagctcctg ttacacctaa gtacataagt gaaaatattt
    10861 caaatataaa taagacaaca gtaaaaggat atgttttatc ttgggatttg ttatcagcac
    10921 caaaagaaaa tattgatgaa attataaaat ggtttcggaa ttagtgaggt gatatagtga
    10981 gtaagatatt agaaatgcaa caaatatata aaagttttgg aaaggtagat gttttaaaag
    11041 ggataaattt tgagttagaa aagggagaag taagagctct tttgggagct aatggagcag
    11101 gaaaatctac acttattaaa attttaggag gagttcattc tcaaactaaa ggtaaagtaa
    11161 ttttgactgg taatgaaata atgtttaaaa attcacatca ttctaaagaa aatggtatta
    11221 gtataattta tcaagaatta agcttgatac caacgcttac agtacttgaa aatatttttc
    11281 ttggtagaga aattttaaaa ggaagtgtgt ttttaaacaa gaaagacatg cttgaggaat
    11341 atatgaaaat ttgtgaggat tttgatttta atatagaccc acatacagta gtttctaatt
    11401 taagtgtagc aaaccaacaa atggttgaga taatgaaagc tgtttcatgt aattctgaaa
    11461 ttatcataat ggatgaacct acaacatcac ttactaataa tgaaaaagat gggctattta
    11521 aaataataag tagactaaag tctaaaggta aaacaatagt ctatatttct cacatattag
    11581 aggaagtttt taaagtatgt gatagtgcaa gtataatgaa aaatggaatt atgattggaa
    11641 cttatgatgt agaggattta actaaattaa agataactca acttatgaca ggaaatgata
    11701 atcacaaact tattgagaag aaggattata catttgcaaa gtatgaagag ccaccagtac
    11761 tagaagtaaa gaatataagc tggtctaaca aaataaaaaa tgtttcattt aaagtttata
    11821 gaggcgaagt tgtaggtctt gctggtcttg taggttcaag aagaactgaa attataaatt
    11881 tgatatttgg tatagataat aaagatagtg gagacatttt tatcaatggt aaaaaagtag
    11941 acataacttc tcctaaggtt gctataaaaa acaaaatagg attgattcct gaagatagaa
    12001 aaaatctagg attaatttta gaacaagcta tatacacaaa ctcatcatca gtacaaattg
    12061 ataaattcaa gaaatttttg tttatagata gaaataaaga aattgagttt tctaaaaaag
    12121 gtgttaagga attaggaata aaagttagtg atgtaaagca agaagtaaaa gagcttagtg
    12181 gtggaaatca acaaaaagta gtagtttcta agtggttaga gaaagattta gatttaataa
    12241 tatatgatga gccgacaaag ggaatagata ttgctgcaaa agaagatatt ttcaatacaa
    12301 tcaaagaatt ttcaagtgaa ggaataggag taatatttat atcttctgat ttagaagaag
    12361 taataagagt aagtgataga attttagtaa ctagagaagg ttctattgta gatgatttaa
    12421 aaaatgagaa tatacaggtt caagatataa tgaacagtat atttaatgta taaaaaatga
    12481 aaatggagga ctgagatgaa gaaagctttt gagaagttaa atttaagaga ttatggagta
    12541 ttattaggtt ttttagcttt atgtatagct ataagtattg caactccatc atttttaggt
    12601 aaacaaaata tacttaatct tttacgacaa tcttctataa ttggaataat ttcagcaggt
    12661 atgacttttg ttataatatc tggaaacttt gatatatcag ttggagcagt agcagctctt
    12721 gcaggtgcta taacaatgaa atttgcgact ggtggaacta atttgtttgc atccatgctc
    12781 ttaggtgtag cgatttgtgc agtaatagga ttaatcaatg gtatagtagt tgcaaagata
    12841 aatgtaccat cattaatagc aactatggct atggtcacta ttgtaagagg attgctgttg
    12901 atgcttacag gaggatatcc aattactgaa aatataccaa tacttgatta cattggaaat
    12961 ggatatttgt ttaatatacc aataccagta gtaattttct ttatagtggt gttagtttca
    13021 tttattgtac ttaataaaac taagtttggt agatatgttt attctgtagg agggaatcaa
    13081 gaagcaagta aacttaatgg tataaatgta gattctcaca aagtgaaagt gtttattatt
    13141 aatgctgtac ttgcaggaat agctggaatc gtactaacag gaagacttgg gactgcaact
    13201 gctattgcag gtgaaggata tgatatggat gcaattgcat ctgtagtaat aggaggtaca
    13261 tcagtggcag gaggttctgg ttctgtactt aaaactgtta taggagtatt acttatgagt
    13321 gttataaaca acagttttaa tcttcttgga atagatgtat atttccagta catatttaaa
    13381 ggattaataa ttttagcagc agttggattt gattcttata gcaagaaaaa acttgcttca
    13441 agataaaaaa taagggggat ttttttatga aaaaattaat gaaacattta gcattgttgt
    13501 tatcacttgt tatgatattt ggtttagttg gttgttcaaa tggtggtgac aaagaaaaaa
    13561 atggagaaaa gaaaatagca gtattactac caggttcaac aggttacttt gtagcaacta
    13621 aacaaggtat agatgcaaaa tctaaagaat taggtgtaag cgtggaatat gcagatgctc
    13681 aatgggatgc tagtaaacaa ttatcacaag ctgaagattt tatggctaaa ggggtagaca
    13741 tgattgcatt atgtggagtt gattctgctg taagtgagag aatagttaaa gctgctaatg
    13801 attcagacgt ccctatagta gcatttacaa attctatagg aagtaaccca actggagaat
    13861 ttaaaggttt agttacatat ataggacaaa atgaggagga aacaggagca ctaactggaa
    13921 aaattgctaa aaacttatta ggagaaactg gtgggaaagc tgtattaata gaaggagtac
    13981 caggaactac tccacaagta ttaagaaaaa aaggtcttga aaaagaatta aaagatagta
    14041 atatagaaat agtttataat caaacttcaa attgggagaa agagcaagct ttaaaagtaa
    14101 cagaagattt aattcaaaag aaaactgatt ttaatgttat aatatgtcaa gatgataatt
    14161 ctgctacagg tgcaggtcaa gctttaaaag atgctggtct taaggataaa gttaaagtca
    14221 taggtttggg tggaagtaaa gatggattaa aagctataac tgatgggtta atagatggta
    14281 caacttatat gtctgctgta gaagaaggtg gattagtcat agaaaaggca tctaaattcc
    14341 ttaaaggtga aaaaatagaa cctgttacac aaattaaaca agttgaagtt aacaaagata
    14401 atatttcaga gttcaagggt gaatggtaaa ttgtcataga taataaataa gatttttaga
    14461 gttactttag aaactatata aagtaactct ttttttaatt taatttagtg ataaattatt
    14521 gaatagtatt tctaaacaaa taaaaaatta atataaacaa agacttaatt atactaaatt
    14581 ataagtaaaa ctaatataaa cttatggaaa aaaggaaaac gtctaaagta ttggaattac
    14641 tatagtgtag gtgtggtatt aaaaaaacat aaaaaaacat aaaaaaattg ttgaaaaaaa
    14701 tatagaaatt ttgtataatt aaagatttga aaagttcggt atacaaagcc aattcttttg
    14761 tatataatat atataaattc agaaaaagga agggatatgt atggccaggc ggttattttt
    14821 agaaaggatt ggacttatat ttttaggttc agcaatatta gcatttggag tttataattt
    14881 ttattattta aacaacataa cagaaggtgg agtgttaggt attttactac tgttaaaaaa
    14941 tttattcaat atacaaccag caatagcaaa tgttgtaata gatggtttac tacttttagt
    15001 aggatataaa tttttcggta aaaaattcct tatatattca atagtagctt ctataacttt
    15061 ttcagtgtta tatgacttat ttgaagctat aggacctcta gtaccacaat cacaaaatat
    15121 gcttttatca actattttgg caggtgtaac tgttggaact ggtgtaggta tagtagtaaa
    15181 agcaggttgt gcatcaggag gagacgatgc tttagcatta gtaatttcta agactacatc
    15241 tttgaacata gggcaagttt atttagcaac agatgttatt gtattgttgt tatcattatt
    15301 ttatctatca gcgtttgata tcttttattc tttgattgct gttacaatta gtggcaaggt
    15361 aattgatttt atttattatc atggtaaaag tttggatatg gatataagta atgatatagt
    15421 tccagaatgt taatataaat taaataactt tagattttat taggggtggc agatttgcca
    15481 ccttttttgt gtctaaatct ttatagaaag tataatccca tctaggtttt tgcaaatctc
    15541 aaatatagaa ttatgttgga atgactggga atacgattgc tagatgtgca ttttaaaaac
    15601 ccaattttat atttatgaaa tactaatcat taatggatgt ttcttaatcc tatctttata
    15661 cctaaaatga taagataaat gaagtagcaa tacaaaacta aaatttgtaa aagagattaa
    15721 attaaatgga ggagctaaaa tgttggatat agttatgata gcattgttat tcataggatt
    15781 tgtttccatg aagttacttg ctgattggtg tgggaaacaa actgagaaaa aataatgtaa
    15841 tgtatggagg gattacctat gtggtttctg gcagttgtaa ttataatgtt aataatatat
    15901 ttggtatatg cgcttattaa tcctgaaaaa ttttaaacag tataaggcta ataagtaagt
    15961 tgaattgggg ggaacaattt ttatggtgca gatagtaata gtgttagcga tttttatgat
    16021 tttagtaata cctatgggca agtatttata tcatatagct actaatcaaa aaacatttgg
    16081 agatagacta tttgataaag tggacaattt tatatataag gtatgtagta tagataagaa
    16141 aaaagaaatg aactggaaac agtatgcatt agctttatta tttacaaatg cagttatggt
    16201 atttatagga tatatcatac ttagaactca aagtatgcat atttttaatc caagtggaat
    16261 taaaagtatg gaacaaggac tatcatttaa tacaataata agttttatga caaatacaaa
    16321 tcttcaacat tattcaggtg aatcaggact ttcatacttt agtcagatga cagtgataat
    16381 atatatgatg tttacatcag cagcaacagg atatgcggct gctatggcat ttgtaagagg
    16441 tctagtcggt aaaaagaaaa cattgggaaa tttttatgta gatttaatta ggataacaac
    16501 aagagtactt ttaccaggag ctttaattat tgggcttata ttagttacac aaggagtacc
    16561 acaaactttt gctgggacag aaacagtgac tactatagaa ggaaagcttc aagatatagc
    16621 tagaggtcct gtggcagctc ttgaatcaat aaaacattta ggaacaaatg gtggtggatt
    16681 ctttggttca aattcatcac acccatttga aaatccaaca ataatatcaa atatagtaga
    16741 aatattatct atgatgatat taccaggagc ttgtgttgtt gcattcggtc acatgataaa
    16801 gaataaaaaa caaggttggg ttgtgtttgg cgcaatgtca atcatatttt taataggtct
    16861 agtagtgtgc tttaaagcag aaagtgcagg aaaccctatt ttatcacaat tagggcttaa
    16921 tcaaagtatg ggaagtatgg aaggtaagga agttagattt ggtatagcac aatcatcatt
    16981 attcacaaca gtgactactt cctttacgac aggtacagtt aataatatgc atgatacact
    17041 gactccttta ggaggattag taccactttt aaatatgatg ctaaatgtag tatttggtgg
    17101 taaaggcgta ggtctgatga atatgttgat gtacgctata atagcagtat tcttatgtgg
    17161 attaatggta ggaagaacac cagagttttt aactaagaaa atcgaaggaa aagaaatgaa
    17221 gcttattgct ttacttatta tattacatcc acttttaata ctaatgttct ctggtctttc
    17281 agtagcaata ccagcagggc ttgaaggaat ttcaaatcct ggattccatg gattatcaca
    17341 ggtattgtat gagtttgcat cttcagcagc caataatggt tcaggatttg aaggtctagg
    17401 agataacact atgttttgga atataacaac aggtatagtt atgttctttg gaagatatgt
    17461 gtctattata gtactactag caatatcaag tttgttagca tctaaaaaag ctgtaaatga
    17521 aagtatagga acacttagaa cggataattt tacatttaca atagttttag tacttgttgt
    17581 tttaattgtt ggtgctttaa catttttccc agccttagcg cttggaccta tttcggagca
    17641 tctagtgttg tggcactaaa aaattgggag ggagtaaagt tttatggaaa ctaaaaaaac
    17701 aaagtttatt acaaaagata ttttcaaaac ttccattata gaagctttta aaaaattaaa
    17761 tcctaaatat atgatgaaaa atccagttat gtttgttgta gaaattggat ttatagtaac
    17821 attgatactt acaatagtac ctagtttgtt tggtgatgaa ggcaataatt taagagctta
    17881 taatgggatt gtaacaataa tcttatttat aacagtcctt tttgcaaatt ttgctgaagc
    17941 tgtagctgag ggaagaggta aagctcaagc agatacactt aagaaaacta gaaaagatac
    18001 agtagcaagg gttatagata cgttaggaaa tgaaaaaatg ataagtgcaa gtgagctaaa
    18061 acttggtgat attgttttag taaatgttgg agaagttatc ccaaatgatg gagaagttat
    18121 agagggcata gcttctgtgg atgagtcagc aataactgga gaatctgcac ctgtcatgag
    18181 agaatcaggt ggagatttcg catctgttac aggtggtact acagttgtaa gtgactggtt
    18241 aaagataaga ataacaacaa aaccaggaga atcgttctta gataaaatga tagctcttgt
    18301 agaaggtgct tcaagacaaa aaacgccaaa tgagattgct ttaaatactt tgttagtagg
    18361 gttaacactt atatttatgg ttgttttaat atctctttat ccaatggcaa actatgcagg
    18421 tgtaaagatt ccaatttcta ctatgatagc tcttttggta tgtttaatac caactactat
    18481 aggtggattg ttgtcagcta ttggtattgc aggtatggat agagtaacta gatttaatgt
    18541 aattgcaatg tcaggaaaag cagtagaagc ttgtggagac gttgatacta tgattttgga
    18601 taagacaggt acgattactt atgggaatag acttgcatca gaatttatag cagttggaaa
    18661 tgcagattca aaagatttaa taaattattc tgttatgtgt tcattaaaag atgatacacc
    18721 agaaggaaaa tctatagttg atttaggtat gaaattaggt tcaacaggta agacaaaaga
    18781 ggcagaggaa gctgagtttg tagaattttc tgcacaaact agaatgagtg gtattaactt
    18841 agctgatggt agagctataa gaaaaggtgc ttatgatagc ataataaaaa gagtaaaaga
    18901 atctggtggt acagtgccag aagatttaga agaaaatgta aatcgagtag ctaaactagg
    18961 aggaacacct ttagtagtgt gtgtagataa tgagatatat ggaattatct acttaaaaga
    19021 tacagtaaaa tcagggcttg tagaaagatt tgctagactt cgagagatag gaataaagac
    19081 aataatgtgt acaggggata atcccttgac agcagctaca attgcaagag aagctggagt
    19141 agatggattt attgcagaat gtaaaccaga agataaaata gatgctataa aaaaagaaca
    19201 attagagggt aaaatagttg caatgacagg tgatggaaca aatgatgctc ctgcacttgc
    19261 tcaggcagat gtaggtcttg caatgaatag tggtacaaca tctgcaaaag aagcagcaaa
    19321 tatggtggat ttagattcag acccaactaa gatattggaa gttgttgaaa taggaaaaca
    19381 gcttttaata actcgtgggg ctttgacaac atttagtata gcaaatgatg ttgcaaaata
    19441 ttttgcgatt ataccggcta tatttactct tgcaatacct caaatggaga tattaaatgt
    19501 aatgaaactt tctacatcat ttagtgcgat attatcagct ttaatattta atgctataat
    19561 tatcccatgt ttaatacctc ttgcaatgaa gggagtaaaa tatagaccta tgaaatcaca
    19621 agctatgtta atgagaaata tgcttatatt tggtcttggt ggagttatag taccattttt
    19681 aggaataaaa ttaattgatt tattgattac acctttagta atgattctta atttaggtta
    19741 ataaatggag gatataaaaa tgaaaacatt aaagccagca ttacttgtaa gtattgtatt
    19801 gttagttgtt tgtggacttg tatatccact tgttttgaca ggtgtaagcc aagttgcatt
    19861 taaagataaa gccaatggaa gtatgataga agtaaatggt gtaaaagtag gttcagaact
    19921 tattggtcaa agctttacag atgcgagatt ctttaaggga agagtatcat cagtaaacta
    19981 taatacttat acaaaagaag atttagtacc agataaagat ggaaatgcat cttatggagg
    20041 tgtatcatca gggtctttta attatggagc tacaaatcca gaacttcatg atagagtaca
    20101 aaaagatata gaaaagtttt taaaggataa tccaactgtc aaaagagagg atataccaac
    20161 agatttactt acagcatcag gttcaggact tgaccctaat ataagtccag cctctgcaaa
    20221 aattcagata ccagctatag caaaagcgtc tggaatatca gaaagtaaat tacaaaaaat
    20281 tgtagatgat aatacttcca aaaaactttt tggagtttta ggtgaagata gagttaatgt
    20341 actaaaggtt aatatagagg tcgcaaaaat attaggtctt atatagtatt ttgtggaatt
    20401 ttagtaacaa aatattactc aagatttaat ttaaaggtta aattttaaag agtgaaaatc
    20461 aataatgatt ttcacttttt tatttgaata ttactaatat ttggaataaa gtgattgtaa
    20521 gtataaaatc caaaaaaagg ttgcattatc aaaatgataa tactataata tatttatcgt
    20581 tatcaatttg ataatagatt tggacatata ggaggaaata gtatgttata taataacgca
    20641 ttagagttga taggaaacac acctgtagta aggctgaata atttaggctg tactaatggg
    20701 tgttctaaca tatatgtaaa attagaaaaa gtaaatccag caggaagtat aaaagataga
    20761 gctgtttatg aaatgttaga gggcttagag caaagaggag aattaaaaaa gggagatgta
    20821 ctagttgaag caactagtgg aaatactggt attgcattat ctatgatagg aaaattaaaa
    20881 ggatatgaag tagtaatcgt aatgcccgaa acaatgagcg ttgagagaag agatttaatg
    20941 aaggcttatg gagcaaattt ggtgcttaca gatggtaaag ctggaatggc aggttccata
    21001 gaaaaagcaa atgaactttt aaaagaaaat ccaaactata aaagcttaaa acaatttgaa
    21061 aataaagata atccaaatgc acattataaa acaacagcag ttgaaattat gaatgatgtc
    21121 aaagatttag atatattcgt atgtggagtt ggaacaggtg gtactttaat aggaactgca
    21181 agatatttaa aagaacaaaa tccaaacata agagtaattg cacttgaacc aaaaaaatct
    21241 ccagctatat cagaaaacaa gactggacct cataaaatac aaggaatagg tgctaacttt
    21301 gttcctgaaa attatgattc tagtgtagtt gatgaagtaa tattagtaga tgatgaagat
    21361 gcatttgata cagttaaatt acttgcagca aaagagggaa ttttagtagg gatatcttct
    21421 ggagcaaata tatttggtgc tatagagatg gcaaaaaagt atccagataa aaagatagtt
    21481 acagtagctc cagatggagt tgataagtat atgtctatgg gaatatttta ataattttat
    21541 ttgagggagt gaatactttg tttaagaaaa taaataaaga tattgaatat ataatgaaga
    21601 atgatccagc agctaggtct aagatagaag tttttttact ttatccatca gttcatgcaa
    21661 tgataatgca taggatggct catgctcttt ataagaagaa gaagcttttt acagcaagat
    21721 taatatccca gatatccaga tttatgactg gcatagaaat tcatcctggt gctaaaatgg
    21781 gggaaggtat attgatagac catggaatgg gtgttgtaat tggagagaca gctgaagtag
    21841 gtaatagggt gacaatatat caaggagcta ctttaggagc tacaggaaaa gatactggaa
    21901 agagacatcc aactgttggt gatgatgtat taattggtgc tggaacaaaa atactaggac
    21961 ctttaaatat aggttcaaat tcaaaaattg gtgcaaattc tgttgtcgtt aaggatgtac
    22021 ctaatggtgc aacagttgtt ggtataccag caaaaatagt aaaaataaga aacctagaac
    22081 ctgtaaaaaa gaataaaaaa gaagtgtcct atgagtatga tgaattagat aatgtatatt
    22141 atatatagga tttatataac tataaaataa actttataaa aattatttta aaaggtattt
    22201 ttttcaaatt tgaggacata ataaaaaaga actgaatgtt acagttttgg ttcatcaatc
    22261 tttgataaac aagattttaa atttgtagcg tctaggagga ttttattatg aaagcaaatg
    22321 ttaatcaaga tacttgtata ggttgtggat tatgtccatc aatatgtcca gaagtatttg
    22381 atatgaagga tgatgggaaa tcacatgtta tagttgatga agtaccaagt gatgctgaag
    22441 aatcagcagc agaagctaga gaaagctgtc cagttgatgc aatagatgtg cattaataaa
    22501 ataaatgcct ataaaaattg aattttcaat ttttataggc atttgtcttt tattcagtta
    22561 gtatttcaac ccctgtttct gtaactaaaa ccatacattc ccattgagca gataatttac
    22621 catcttttgt ataaatagtc caattatttt cttcatcaac aaaaatttcg tgagttcctt
    22681 catttaccat aggttctatt gtgaatatca tcccaggaac caatagcatc tcagtccctt
    22741 ttttagatac ataacttaca aaagggtctt catgaaagct taatccaact ccatgaccac
    22801 ctatttctct aacaacagag tatccatttt tctttgcata agagttgata gcatcaccta
    22861 tatctcctaa aaatccccat ggtttggcaa cttcaagacc tttttggaca cattcctttg
    22921 taacttgaac taattttttt gcattttcat taacattacc tatcataaac attcttgaag
    22981 catctgaata gtatccatta aaaatagtag aaacatctac atttataata tctccatctt
    23041 ttaagataat gtccttgctt ggaataccat gacaaacttc attatttact gatgtacata
    23101 cacttttagg aaatccatta tagtctagtg gagcagggat agctccattt tgtagtgtaa
    23161 agtcatgaac caacttattc atatcttctg tagacattcc aatttcgatt ttatctgaaa
    23221 tataatccaa aatttttgtg tttattacag cacttttttt tatttcctca atttgacttt
    23281 cattttttat aatatcatga gttggtacaa tatgacctaa cttagcaaat gaatctattt
    23341 tattatccat atccatatga cattttttat attttttagt acttccacac caacagctat
    23401 catttctatt taacttcata acttttcatc cttcttttat tatttattta aattatgggt
    23461 tacataaact attttaaaac ataaccatta taaatattat aacataaata ctaagaaatc
    23521 ctttatagat agtgatttat tcaaagtata aattattata tggttattgt tgatgaaaag
    23581 aggatagtta aatgtattat tggatattag cgcacttaat agtttagttc aagtttttca
    23641 agaaaatcat atgttagagg acaattttcc tttaaagtat tttttgaatc ttcatcaaga
    23701 taataatgca caaatgattc tgcaaaaaat tcttctataa acttgcttat atatgcatgt
    23761 gcaacttgtt tagggtcata aagcttatat ctttcctgag caaatacttc cataaattct
    23821 tcagaatttg attttttatt taaaacattt ttatctatag catgtgcagt ttcatgtaat
    23881 tctaaatttt ttgatgaatg tacatttgca tagctagggt tactataacc tattttagct
    23941 actatagaat tatttctata tataccaggt acatctttcc aagtcttgcc tgatcttacc
    24001 caacatggag gtaattgata ttttaaatca ctaaattctg gttcatcagt tagatttgaa
    24061 tttattaatt ttatttttat atccttttct ttaagtgtct gtaaatattt aatatttatt
    24121 tttgcaagtc tgcttttcat attttcaact tcatctttat cataattcaa gtcatcagga
    24181 agtataagca tttgttcaat tatatctatg ctactgtctt ttatatcttc agattcttca
    24241 tcaaatttat attgaagagt attattttca tagatagtta tatttgtatt attttccata
    24301 taataagagt gaatatcctc taagtctaaa atattcattt gttgtaaaat atgttttgtt
    24361 tcgccactcc atactactaa agaaagtgaa ttaaaattaa ttagtggatt ttcctttgct
    24421 tttatatcat tgcttatatc tacatcaaat acatattcaa ttgagtcttc tatgttaaga
    24481 ctatctgtat tctttttaat atataaatat gcttgtttta agtctatata aatctcatct
    24541 aaagattcat cataaaattc tgataaatta ttagtaaaga gttcttcact taaaagttca
    24601 tttttatcag ttatactttc aattaaaata ttaatttctt ttatcatatc gtaaatactt
    24661 atttttctat ttgctttttc actagataca ttgcatggcg caagagcaat gcaataaaaa
    24721 tctttttctt ttttataaaa ggctttaagt gattttgata aaagtgtaaa gtctacacct
    24781 atctgtaagt tgaccttaag tgagtcattt tgtaataatt cagtattatt ttttttagct
    24841 atatttaaat tgttttccat aatcattgcc ccctttttat tagggtatca taattttttt
    24901 atatgtgtca atttgttttt ttgaaaatta taatttttt
//
LOCUS       DAMJWP010000038        24368 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.039, whole genome shotgun sequence.
ACCESSION   DAMJWP010000038 DAMJWP010000000
VERSION     DAMJWP010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24368
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.039"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            97..3045
                     /locus_tag="PC700_001306"
     CDS             97..3045
                     /locus_tag="PC700_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2580622.1"
                     /translation="MRQRIKNLIENEDKKNPLTDEAISSRLNIRREDVTFLRNELKIE
                     DSRQRRKVVLIKAIKDILKEEKTINKNDITRRLNSIGFTVSRFTVIQYLKEIQDSGDL
                     KLDKESDDKKEEIINNRENKDIAFDKLIGSSGSLSTIIKLAKAAILYPPHGLHTIIMG
                     PTGVGKSELAECMYKFAVESSRFPKNAPFIVFNAADYSENPQLLLSQLFGHVKGAFTG
                     ADENKEGLVSKADGGILFIDEIHRLPHEGQEILFQLIDKGMVRKLGETRLTHKIDVMI
                     ISATSEPIDSHLLNTFKRRIPVTIEIPELVARPLNERFDIINNFFLIEAQRMGANIHI
                     KSDVLKALMLYDCIGNVGQLRSDIQVACARGLLNQLTNKLKEVNISISDVPGYVKQGL
                     IKIRNCRGKIENYVDGDLIIDSNLQGEIEKKNEDIYTFPDEIYKLTERRHIDLLEQGL
                     DFDVINRIIGGELESCIQKYIKQVKKVSISNNLPDISPIVGKYIVELTYEVIKIASQY
                     LSSLEPNLHLCLAVHLKAAFERLKEGKVISNAHLKEIIGNYPLEYSVAEKVAQYLKER
                     YNINLPKEEIGIIAIYFKMSSRRDMSEQKKIGVLLMAHGRVASSVCEVTNKLLGINHA
                     RYLDMPFEKKPEEMIEEATTIIKDIDEGRGVIILVDMGSLSYFGDIISGKTGINIRTI
                     SRFDTLLSIEVIRRAVLPDLTIDEIVDEVKGIEPQVKEKVSKCNNTKVDFRKPIILTL
                     CITGLGGACKIKKILEENIKSITEKVDIEPIGMIEYPSLDLEIKQISNDKNVIAIVGT
                     IDPQIANIPFFSLVDINDKAKLNQINNLIDTNQIGETNKKYELSDLIHKEDIFVDLQV
                     KSKEEVLKYMSKKLREKGYVTKNFYKKVVERDNLFPTELSDEIAIPHTDGIDVIRPAI
                     SIAILKKPIVWSKSKVKIILLLAVDQKCFNPLSTLLSFIETDEFKKIKNIKDRDFIRE
                     MILDGVNENN"
     gene            3023..3487
                     /locus_tag="PC700_001307"
     CDS             3023..3487
                     /locus_tag="PC700_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2580623.1"
                     /translation="MESMKIISSNLIFKNIEVSNNEDALKFLGQRLFDEQYVKESYIQ
                     AVVAREKKYATGLPTEIYGVAIPHTDIVHVNEPGIAIGILNKPVKFIMMGTDDTEIDV
                     KVVFMLAVKEPQEQLQLLEKLMTIFQDKNILNNIVDLSEESVSDLLNSKLKN"
     gene            3600..3902
                     /locus_tag="PC700_001308"
     CDS             3600..3902
                     /locus_tag="PC700_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2580624.1"
                     /translation="MGVTNVTKKILVACGTGVCTSTIAINKLKKALQDIGKLDMVNIT
                     QCKVVEVASKAPDYDLIICTTQVSSSIKTPVINGLPFLTGVGMDKLINDVLEELEL"
     gene            3964..5328
                     /locus_tag="PC700_001309"
     CDS             3964..5328
                     /locus_tag="PC700_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425524.1"
                     /note="with GatAB forms a phosphoenolpyruvate-dependent
                     sugar phosphotransferase transporter for galactitol;
                     subunit IIC forms the translocation channel and contains
                     the substrate binding site; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDF2580625.1"
                     /translation="MEGLYMEILNFIVGLGAQVMMPIIICIFGLILGTKLGKSLRAGL
                     TVGVGFIGLNTIIALLTDNLGPATQQMVKNLGLSLSIIDVGWPAASAIAFASTVGALI
                     IPIGLVVNIVMLITNTTQTVDVDIWDYWHFAFTGALVAGATQSVMWGVFAAVANMVIV
                     LVMADLTAPGIEEYLGMPGISLPHGFTQAFVPIAIVVNKLLDLIPGINKIEINADTLQ
                     KKFGLFGEPLIMGSVIGVIIGIAAKYDIKGILQLGVTMGAVLILIPKMAALLMEGLLP
                     VSEAAQEFIEKRFKNRGKIYIGLDSAVGIGHPVTLSVALVLVPLTILIAAILPGNKVL
                     PFADLAVIPFALVLIVPITKGNVFRTLIIGIIIITSGLLIATNLAPLFTQMALNASFK
                     MPEGATMISSICDGANPLSWVFVKVMNYKVIGGVVFGVIALGMAIYNRNRIINENKKL
                     SIEE"
     gene            5363..5836
                     /locus_tag="PC700_001310"
     CDS             5363..5836
                     /locus_tag="PC700_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580626.1"
                     /translation="MKKYKYDSKRYLLSVTIPGFFMILILIYALFNNYINFNLNIYSL
                     LIIVCTYGIFNTFISSSNPKKIIIDSKCIHFTSFMTTHKYEIKDIEKIRIREFYNKKI
                     YLKINDGDLFKGRYWIRTNMFNNSIALYSYFIELEECLYPDSLRFRNKKFENKNI"
     gene            6008..6658
                     /locus_tag="PC700_001311"
     CDS             6008..6658
                     /locus_tag="PC700_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HDF2580627.1"
                     /translation="MLLEKLRKEVLQASLDLLNYNLVTLTGGNVSGRDEQTGYIAITP
                     SGMDYRNLTPSDIVIVDVDGNIIDGKWKASVDLSDHLYIYKHREDINSIIHTHSTYSS
                     CFAILNEPIECASTTLANEVGGSVPVAKFSPPTFKKMGKCVIEAIGDKRACLLANHGV
                     IAVGPSVGHALTAAVMLEDSAKVYYLAKSIGTPVLLPDEEIQRARDVFFNVYGQDK"
     gene            6832..7764
                     /locus_tag="PC700_001312"
     CDS             6832..7764
                     /locus_tag="PC700_001312"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDF2580628.1"
                     /translation="MYYLLNKSTNPYFNLALEEYLFLNDKYNDDIIIIWRNEDSIFIG
                     KNQNPYQEVHHDVIEKGEIPILRRISGGGTVYHDLGNINMSFIQKDRQLHEIDFLEHT
                     KFMQKMLSTLGLDVSITERKDLFLNGKKISGSAQSIKRKNSLYHGTLLYDSDLNKLTK
                     YLNSNKATESNATKSVSSKVTNIKFLLEKDINYFLDYCVEYLKSNIKNIKELELDEED
                     IKSVYNIGEDKYRKIEWTYGKTPKFQVVLPFNNESKINIHVNRWKVSKFFISYDDNVI
                     NMDKLLDLNFFKEEIKESILENYPHYIDLINLIF"
     gene            8038..8751
                     /gene="ispD"
                     /locus_tag="PC700_001313"
     CDS             8038..8751
                     /gene="ispD"
                     /locus_tag="PC700_001313"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HDF2580629.1"
                     /translation="MYSVIIVAAGSGRRMNLDINKQFIKLREKEIIAHTIQVFYENIN
                     IDEIVVCIKKEEEDFFKENIINKYNFKNIKIAYGGKERQDSIYNGLKKLDENCDIVLI
                     HDGARPFVDHRIINESIKVAKEKKAVVVGVPVSDTIKIVSDGTVQETPERNLLWAAQT
                     PQTFEYNLIIDAYEQAYKNNYYGTDDSMLVENIGQSVTMVMGSYENIKITSPEDLNIA
                     EQILNMEKRDEVNSRRRII"
     gene            8748..9233
                     /locus_tag="PC700_001314"
     CDS             8748..9233
                     /locus_tag="PC700_001314"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HDF2580630.1"
                     /translation="MRIGLGYDVHKLTENRKLIIGGVEIPHDKGLLGHSDADVLIHAI
                     MDSILGALALGDIGKHFPDTDEEYKGADSMKLLEHVYNLITSKGYKIGNIDSTIIAQS
                     PKMAPYIESMRSNISKVLNTDIDNINIKATTEEGLGFTGAKQGIASQSICLLLLTSQN
                     N"
     misc_binding    9328..9536
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            9600..11315
                     /locus_tag="PC700_001315"
     CDS             9600..11315
                     /locus_tag="PC700_001315"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDF2580631.1"
                     /translation="MKMSKMFMPTLKEIPADAEITSHQLMVRSGMIKKMTSGVYNQLP
                     MGLRVFKKIEQIIREELNKKDCQEILCAALLPSELWKESGRWTAMGEEMFRLKDRTER
                     EYCLGPTHEEAFTDIIRQEITSYKQLPLNLYQIQVKYRDERRPRFGVMRTKTFTMKDA
                     YSFDVDDKGLDKSYQDMFDAYVSIFDRCGLENSPVQADSGAIGGSTSAEFMVKSEVGE
                     DEVVFCSGCDYAANVERAESCNLASQKEEMKELEEVHTPGAATIKELEEFLKTSPDKF
                     AKTLVYEADGKTVVVVVRGDREVNEIKVSNAIGSVIEFALATDDVVRKVTNAEVGFAG
                     PIGINADYVFIDKEIVEQRNIVVGANKTEYHIKNANYGRDFEGIVGDFRNVQEGDQCI
                     VCGKPLEIARGVEVGHIFKLGTKYSESMNANFIDKDGKSKPIVMGCYGIGVERTAAAI
                     IEQHNDEKGIIWPLSVAPYHVVIVPANMKNEEQISIAENIYNDLQAMGVEVLLDDRDE
                     RIGVKFNDSELIGIPMRITVGKNINEGKVEFKLRHKEDKEIIDIEEINEKVKAEFIRN
                     NVRLG"
     gene            11437..12882
                     /locus_tag="PC700_001316"
     CDS             11437..12882
                     /locus_tag="PC700_001316"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDF2580632.1"
                     /translation="MAKNEKQFVEEITKMEDDFPQWYTDVITKTDLVDYAPVKGFMVV
                     KPYGYALWEKMQEFMDKKFKETGHKNCYFPLLIPESLLNKEAEHVEGFAPEVAWVTHG
                     GNKKLEERLCVRPTSETIICTMYAKWLKSYRELPYLYNQWCSVVRWEKSTRPFLRTSE
                     FLWQEGHTLHETAEEAQEETIQQLEVYKALCEELLAMPVVAGQKSESEKFAGGERTYT
                     IEAMMHDGKALQSGTSHFLGQHFTKAFDITFADREGNLANPYHTSWGASTRLIGGLIM
                     THSDNRGLVLPPRVAPIQVVIVPIAAKKGNVMETVDKIYADLKTKGVAVEVDDRDNYT
                     TGWKFNEWEMKGAPVRVEIGPKDIENNQAMVFRRDTLEKDSMPLEGLADAICDLFDVI
                     HNDMFEKARKHREDNTSIVENMDEFRKALEEKPGFIKTMWCGDAECEAKIKEETGATI
                     RCLPFEQENLGHKCVYCGKEADSMVVMAKAY"
     gene            13322..14803
                     /locus_tag="PC700_001317"
     CDS             13322..14803
                     /locus_tag="PC700_001317"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDF2580633.1"
                     /translation="MNVRVRFAPSPTGFVHIGSLRTALYNYLFAKKMGGEYILRVEDT
                     DQSRLVEGAIENMLNAMKWAGVNHSEGVILDDSGKVVQKGEYGPYIQSQRLDIYQEYI
                     KQLLDSGKAYYCFCTKERLDEVRDAQRAAGETAKYDGHCKNLSKEEVEANIKAGIPYV
                     IRLRLPENHTIKFTDLVRGDMEFNTNDLDDQVLMKTDGFPTYHFAVVVDDYLMKITHV
                     IRGEEWVSSTPKHVYLYEAFGWEAPVFVHLPNILNKEKKKLSKRQGDVAVEDFKKKGY
                     LPEGLVNYVALVGWSPEDNQELFTMEELEKAFSVERVSKSGGVFDTEKLNWVNQHYIK
                     DGDDAYLTDLAIPFLVEDGFITEEEATNKYDFLKSMISVLKEKLQYVKEITEHASIFF
                     GDKIEVETEEGREFLRLEHIPTLIDALREKIEKTEVLNAEFVQAMLKEIQKEYKIKGK
                     NLFMGSRIILTGQMHGPDLPKVMEVLGKETCLNRIAYVKNNIL"
     gene            15024..15179
                     /locus_tag="PC700_001318"
     CDS             15024..15179
                     /locus_tag="PC700_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580634.1"
                     /translation="MYSNIDDVKKELKELCLEYVTILEKLKDEKMITEETFEKCSSQK
                     KIFLEEQ"
     gene            15324..16721
                     /locus_tag="PC700_001319"
     CDS             15324..16721
                     /locus_tag="PC700_001319"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDF2580635.1"
                     /translation="MKVYNTLTRTKEEFVPLEEGKVKMYVCGPTVYNYIHIGNARPFI
                     IFDTLRRYLEYRGYDVTYVQNFTDVDDKIINRSHEEGISPEEVAAKYIKEYFVDCDGL
                     GIKRATVHPQVTDNIQQIIEFIKELEDKGYAYAVNGDVYFDTNKFEGYGKLSGQKQED
                     LEAGARIEVNDQKRHPMDFVLWKAKKEGEPGWDSPWGEGRPGWHIECSVMSKRYLGET
                     IDIHAGGQDLTFPHHENEIAQSEARSGKIFSKYWMHNGYININDEKMSKSKGNFFTVR
                     DISKLYDLEIVRFFMLSAHYRNPVNFSDEMLNQAKAGLERLYNTKEKLEFTLSNLVES
                     PLTEKEVELVKELDDFRQKFIDAMDDDVNTADAVSVIFELAKLINSNVDENSSLEFAK
                     KCLDEFNELTGVLNIVNKKKDTVLDKDIEELIQKRTDAKKNKEFQLADDIRQQLLDMG
                     IVLEDTRQGVKWKRI"
     gene            16706..17119
                     /locus_tag="PC700_001320"
     CDS             16706..17119
                     /locus_tag="PC700_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HDF2580636.1"
                     /translation="MEKDIKMEKTELVTMSPLVLAYLGDTVYETYIREYLIRQNTQRK
                     VNDLHKLAIKYVKAKAQATIIHEIEIELTEEESKIYKRGRNQKSNTSPKNADIIDYKH
                     ATGFEALVGYLYLNNEIERLQYIINKGIKIIERDM"
     gene            17226..17321
                     /locus_tag="PC700_001321"
                     /pseudo
     CDS             17226..17321
                     /locus_tag="PC700_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452247.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase (FAD)"
     gene            17627..18457
                     /gene="thyA"
                     /locus_tag="PC700_001322"
     CDS             17627..18457
                     /gene="thyA"
                     /locus_tag="PC700_001322"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDF2580637.1"
                     /translation="MSLADKIFIDMCNNILENGVSSDGEVVRAKWDDGQPAHTIKKFC
                     VVNRYDLSKEFPILTLRPTNLKAAIDELLWIWQKKSNNINDLNSRIWDSWADETGSIG
                     KAYGYQIGQKHKYKEGEFDQIDRVIYDLKYNPYSRRIITNIYNHNDLNEMNLYPCAYS
                     MTFNVTGNRLNAILNQRSNDILVANNWNVTQYAILVHMLAQISDLEVGELVHVIADAH
                     IYDRHIPLVKELINRESYDAPKLIINPNIKNFYDFRVEDFTLENYKSGPQIKKIPVAI
                     "
     gene            18470..18964
                     /locus_tag="PC700_001323"
     CDS             18470..18964
                     /locus_tag="PC700_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDF2580638.1"
                     /translation="MKVIVAVDLNWGIGYGGKLLQSIPEDMKFFKEKTVGNVVVMGRE
                     TFKSLPGKKALKDRVNIVLTRSKSFNDDRLIVCNSIDETLKELQKYENDKIFIIGGET
                     VYKQFLPYCNELYVTKIQKTYEADRFFPNIDIMSNWDLIKESEVKEYNNIRYVFSTYI
                     NNSL"
     gene            18991..20367
                     /locus_tag="PC700_001324"
     CDS             18991..20367
                     /locus_tag="PC700_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HDF2580639.1"
                     /translation="MKKDIFVNRVSNALENDRGAVFVGSGISFSSTEVDWFKLLEPLV
                     NELDITLDNKSDDLPMIAQYIVNNYSGNRGPLVNEISKTFNRKFKLNKYHKYLATTKL
                     STIWTTNYDMLLEDAFYDFDTSVKVSDDSITRNRLEYDVEIIKMHGCISRSDWNEIVI
                     TQEDYDDFSINKPAISQRLSEDLINKSFLFIGYSYRDPNIRNIMINARRLSKKHTQQH
                     YIILKKLKDSDSNITQDDVVRQELWCNDLKRLGISTLFIDEYSELEELLREISQKSRG
                     KTVYVTGSHKESNMNARKLGTKLAKHKDITLLSGQSSGIGSEVVAAFTEECIKSKEDI
                     INRMKIFPNPYSADEKYSDDITLIPDLKKCRTKMLNSTQVVVVFSGGIGTEAEIDVAI
                     KQNCNIIPVITCKEDRENPAIKKILNYSDNMKNIEIKDATYYNKIVDEDGVVTMEEIY
                     ICISKIMN"
     gene            20343..21443
                     /locus_tag="PC700_001325"
     CDS             20343..21443
                     /locus_tag="PC700_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HDF2580640.1"
                     /translation="MHIKNNELKHDIIKLKKRNGFIEVGKDKKTLVNCLIGANELEDY
                     KYEIKKIDCILNISEKPDIVSDLSTKRRKISESIWYKIVKDTPFVSATLPIYLVSKNN
                     NKIDSNELLDIIIEQMENGVGMITIHPTANKQIFEDSKNRMVPVTSRGGGMVIQDLIS
                     KNFSEDNVYLKILPEIIKYAQKHNVCISLGATFRSGNIFDSNDRAQITEIKSQIELAK
                     RIYKSNVGVIIESPGHSRPSDIIKISSILKKEGFPIMPLGPIPTDIAIGMDHIAAAIG
                     ATIMGIEGCANIISTVTREEHTGGRPSIESTVESIRTAKIAAHIIDIHQMNDINLDMS
                     VSKSRAKSNTCIFGKDTRYCDRCRELCPLCIR"
     gene            21486..21911
                     /locus_tag="PC700_001326"
     CDS             21486..21911
                     /locus_tag="PC700_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HDF2580641.1"
                     /translation="MHRIASFSQQSQRYVKLEQFEYIIPPEIEKIEKAKELFINSMKK
                     YQEDYDKLVEILFENHYNKLIKGGKNEKTSKRQVEKKAIEDARYVFPNACETKMVFTI
                     NARSLFNFFEHRCYENGSCLEGTMTCGDIVQVRKKFKNL"
     gene            21924..22661
                     /gene="rlmB"
                     /locus_tag="PC700_001327"
     CDS             21924..22661
                     /gene="rlmB"
                     /locus_tag="PC700_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDF2580642.1"
                     /translation="MASIEGRNPVIEAIKSDREIDKILIANSAKEGSIKKIIGMAKDK
                     NIIIQYVDKHKLDEVSTSHSHQGVIAYASEYKYYELDELIDLAKNKDEDPFFIILDEI
                     TDPHNLGSIIRTADAVGAHGVIIPKRRSVHITPVVAKASAGAVEYMPVCKVTNIVNTI
                     KRLKEEGLWIAAADMDGETFYKQNLTGPLGVVIGSEGFGISRLVKQNCDFIVKMPMIG
                     NVTSLNASVAGGILLYEIFRQRLDKSK"
     gene            22664..23191
                     /locus_tag="PC700_001328"
     CDS             22664..23191
                     /locus_tag="PC700_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HDF2580643.1"
                     /translation="MKKNINHYLIIDGYNIINAWDNLKELAKEDLEDSREKLIDDIIE
                     FSEFMGYKTIIVFDAYNVKNSREKVEKRKHITIVYTREHQTADSYIEKFITSLSKYDD
                     VKVATNDYAEQQMILGKGATRMSARELKLELDRSKNKMKEKNIGLRKKIQRNWLEERL
                     DKETLSKLENIRRKR"
     gene            23256..23903
                     /gene="sigH"
                     /locus_tag="PC700_001329"
     CDS             23256..23903
                     /gene="sigH"
                     /locus_tag="PC700_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HDF2580644.1"
                     /translation="MLVAKEKSYELVDNCQQDEYNIVLRASEGDKIALEYIITKYRNF
                     VKAKAKSYFLIGADKEDIIQEGMIGLYKAVRDFDGSKTNSFKCFAEICITRQIITAIK
                     TATRQKHIPLNSYVSLNKPIYDEESDRTLLDIIATSIVTDPEELIISKEELKNIESKM
                     NELLSDLELEVLELYLNGKSYQFIADKLKRDVKSIDNALQRVKRKLEKHLENRND"
     gene            23977..>24368
                     /locus_tag="PC700_001330"
     CDS             23977..>24368
                     /locus_tag="PC700_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HDF2580645.1"
                     /translation="MAKAKYERTKPHVNIGTIGHVDHGKTTLTAAITKTLYDRYQLGE
                     AVDFANIDKAPEERERGITISTAHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVCSATDGPMPQTREHILLSRQVGVP"
BASE COUNT        10094 a         2524 c         4521 g         7229 t
ORIGIN      
        1 taaatgttat tactttataa aaaaataata agtacaaaaa aaacaatata tggcatataa
       61 gttgctatat aaataataag gagggaacaa ttataaatga ggcaaagaat aaaaaactta
      121 attgaaaatg aagataagaa aaatccttta acagatgaag cgatttcaag taggttaaat
      181 attcgaagag aagatgttac ctttttgaga aatgagttaa aaatagaaga ttcaagacaa
      241 agaagaaaag ttgttttaat taaagctata aaagatattt taaaagaaga aaaaactata
      301 aataaaaatg atataacaag aagattaaac agcattgggt ttacagtatc aagatttaca
      361 gtcatacaat acttaaaaga gattcaagat tctggtgacc taaaacttga taaagagagt
      421 gatgataaaa aagaagaaat aatcaataat agagaaaata aagatatagc atttgataaa
      481 ctcataggta gtagtggtag cttaagtaca attataaaac tagccaaagc agcaattctt
      541 tatccacctc atggtcttca tactattatt atggggccaa ctggtgttgg aaagagtgag
      601 ttagcagagt gtatgtataa atttgcagta gaatcatcca gattccctaa gaatgcacca
      661 tttatagttt ttaatgcagc agattattca gagaatccac agcttttgct atcacagtta
      721 tttggacacg ttaaaggtgc ttttacagga gctgatgaga acaaagaagg attagtatca
      781 aaggcagatg gaggaatatt gtttatagat gaaatacata gattacctca tgaaggtcaa
      841 gaaatactat ttcaactgat tgataaaggt atggtcagaa agttaggaga aacaagacta
      901 acacataaaa tagatgtaat gattatttca gcaacttctg agccaataga ttctcattta
      961 cttaatacat ttaaaagacg tatacctgta acaattgaaa taccagaatt agttgcaaga
     1021 ccattaaatg agaggtttga cattataaat aatttcttct taatagaagc tcaaagaatg
     1081 ggagcaaaca tacatataaa gtcagatgta ttaaaagctc ttatgcttta cgattgtata
     1141 ggtaatgtag gtcaacttag aagtgatatt caagtagctt gtgctagggg actattaaac
     1201 caacttacaa acaaactaaa agaagtaaat ataagcatat cagatgtacc tggatatgta
     1261 aaacaaggtc ttataaaaat cagaaattgt agaggtaaaa tagaaaatta tgtagatggg
     1321 gatttaatta tagattcaaa tcttcaaggt gagatagaaa agaaaaatga agatatatat
     1381 acttttccag atgaaatata caaacttact gaaagaagac atattgattt attggaacaa
     1441 ggtttagatt ttgatgttat aaatagaatc attggaggag aattagaaag ttgcattcaa
     1501 aaatacataa aacaagttaa aaaagtttct attagtaaca atcttccaga tataagtcca
     1561 attgttggaa aatatatcgt agagctaact tatgaagtaa tcaaaatagc atcacaatac
     1621 ctaagttcac tagaaccgaa tctacattta tgtttagcag ttcacctaaa agcagctttt
     1681 gagagattaa aagaaggaaa agtaatatca aatgctcatt tgaaagaaat aataggaaat
     1741 tatccactag aatattctgt agcagaaaag gtagctcaat atttaaagga aagatataat
     1801 ataaacttac caaaagaaga aataggtata atagcaatat attttaaaat gagtagtaga
     1861 agagatatgt ctgaacaaaa aaagataggt gtacttctta tggctcacgg aagagtagca
     1921 tcttctgtat gtgaggttac aaataaacta ttaggaatta atcacgcaag gtatttagat
     1981 atgccatttg agaagaagcc ggaagaaatg attgaagaag ctactacaat aataaaagat
     2041 attgatgaag gtagaggtgt gattatatta gtagatatgg gttcactttc atattttgga
     2101 gatattattt ctggaaagac tggaattaat ataaggacaa tatctagatt tgatacattg
     2161 ctttctatag aggttatacg aagagcagtt ttaccagatt taacaataga cgagatagtt
     2221 gatgaagtta aaggtataga gccacaagta aaagaaaagg ttagtaaatg taacaataca
     2281 aaagtagatt ttagaaaacc tataatttta acattatgta ttactggatt agggggagct
     2341 tgtaagataa aaaaaatcct agaggaaaat attaaaagta taactgaaaa ggttgatata
     2401 gaacctatag gtatgattga atatccaagt cttgatttag agataaaaca aataagcaat
     2461 gataaaaatg taatcgccat agttggaaca attgaccctc agatagcaaa tatccctttt
     2521 tttagtctag tagacataaa tgataaagca aagttaaatc aaataaataa cctaattgat
     2581 acaaatcaaa ttggtgaaac aaataaaaaa tatgaattat cagatttaat acataaagaa
     2641 gatatatttg tggatttaca ggttaaatca aaagaagaag ttctaaaata tatgagtaaa
     2701 aaacttagag agaaaggtta tgtgacaaaa aatttctata aaaaagtagt ggaaagagat
     2761 aacttatttc caactgaatt atcagatgaa atagcaatac ctcacacaga tggaatagat
     2821 gtaattaggc cagctataag tatagccatc ttaaagaagc ctattgtgtg gagtaaaagt
     2881 aaagtaaaaa taatactttt attagctgta gaccaaaagt gttttaatcc attaagcact
     2941 ctactctcat ttatagaaac agatgaattt aagaagataa aaaacattaa agatagagat
     3001 tttattaggg agatgatatt agatggagtc aatgaaaata attagtagca atttaatttt
     3061 taaaaatatt gaagtgtcta acaatgaaga tgcattaaag tttttgggac aacgtttgtt
     3121 tgacgaacag tatgtaaaag aaagctacat acaagctgtt gtagcaagag aaaaaaagta
     3181 tgctacagga ttaccaacag aaatatacgg agtggcaata ccacatacag atatagtaca
     3241 tgtaaatgag ccaggaatag caattggaat actgaataaa ccagtaaaat ttataatgat
     3301 gggtacagac gatactgaaa ttgatgtaaa agttgtattt atgttagcag tgaaagagcc
     3361 acaagaacaa ttgcaactat tagaaaaact tatgactata tttcaagata agaatatact
     3421 aaataatatt gttgacttaa gtgaagaaag tgtgagtgat ttattaaact ctaaattgaa
     3481 aaattaaaca tgtaaagtat attaaaaatg taaaattaaa catgcaaagt atgttaaaaa
     3541 atataaaaag attattaaag atttctaact atagatatct ttaaaataaa ataatttcaa
     3601 tgggggtgac taatgtgact aaaaagatat tagttgcatg tggaacagga gtatgtactt
     3661 caacaattgc tataaacaaa ttaaagaaag cacttcaaga cattggtaaa ttggatatgg
     3721 ttaatattac acaatgtaaa gtagtagaag ttgcatcaaa agcacctgat tatgatttaa
     3781 taatttgtac tacacaagta tctagtagta taaaaacacc agtgataaat ggattacctt
     3841 ttttaactgg tgtcggaatg gataaattaa tcaatgatgt attagaagag ttagaattat
     3901 aattaaaaaa attaaggaac atggtctcta aaatcataag ttttagagac ttaaaattaa
     3961 ataatggagg gtctttatat ggagatatta aatttcattg ttggtctagg tgcacaagta
     4021 atgatgccaa taataatatg tatttttggt ttgatattag gtacaaaact aggaaaatct
     4081 ttaagagctg gtttaactgt gggagttggg tttataggtc ttaacactat tatagctttg
     4141 ctaacagata atttaggtcc agctacacaa caaatggtta agaatttagg gctaagttta
     4201 tcaataatag atgtaggatg gccagcagca tcagctatag cttttgcatc aacagtaggg
     4261 gctttaatta ttccaatagg tcttgtggta aatatagtta tgctaattac taatactact
     4321 caaactgttg atgtagacat atgggattac tggcattttg cttttacagg agctttagtg
     4381 gcaggggcta ctcaaagtgt aatgtgggga gtatttgcag cagtagcaaa tatggtaatt
     4441 gtattggtaa tggcagattt aactgctcca ggtatagagg aatatttagg tatgccaggt
     4501 atatctcttc ctcatggatt tacacaggca tttgttccaa ttgctatagt agtaaataaa
     4561 ttattagacc taataccagg tataaataag attgagataa atgctgatac attgcagaaa
     4621 aaatttggat tatttggaga acctttaata atggggtctg taataggtgt aataattggt
     4681 attgctgcga aatatgatat aaaaggaata ttacaattag gtgtaactat gggagctgta
     4741 ttaatattaa ttccaaagat ggcagcatta ttaatggaag gattattacc agtatcagaa
     4801 gctgcacagg aatttattga gaagagattt aaaaacagag gcaagattta tataggctta
     4861 gattcagcag taggaatagg tcatcctgtt acattatcag ttgcactagt gttggttcct
     4921 ttgactatac ttatagcagc tatattgcca ggaaataaag ttttaccatt tgcagattta
     4981 gcagtaattc catttgcact tgtattaatc gtaccaatta caaagggaaa tgtgtttaga
     5041 acattaataa taggaataat tataattact tctggattat taatcgcaac taacttagct
     5101 ccattattta cccaaatggc tttaaatgct agctttaaaa tgcctgaagg agcaactatg
     5161 atttcaagta tttgtgatgg tgcgaatcca ttaagttggg tatttgtaaa agttatgaac
     5221 tataaagtta ttggaggagt agtttttgga gttatagctt taggaatggc tatttacaat
     5281 aggaatagaa taataaatga aaataaaaag ctatctatag aagaataagg ttaaaacaat
     5341 tttattaagg agtgtatagg ttatgaaaaa atataaatat gattctaaaa ggtacttact
     5401 cagtgttaca ataccaggat tttttatgat tttaatattg atttatgcac tgtttaataa
     5461 ctatataaat tttaatttga atatatatag tttgttaata attgtctgta cttatggtat
     5521 attcaatact tttatatcat caagtaatcc taaaaaaatt attattgaca gcaaatgtat
     5581 acattttacc tcatttatga ctacacataa gtatgaaatt aaagatatag aaaagataag
     5641 aataagagaa ttttataata aaaaaattta tctaaaaatt aatgatggag acctttttaa
     5701 aggtaggtat tggataagaa caaatatgtt taataatagt atagcattat atagttattt
     5761 cattgagttg gaagagtgct tatatccaga ttcactaaga tttagaaata aaaaatttga
     5821 gaataagaat atataaaaat aaaaaattga attgaagcct aacttaatct tttttgataa
     5881 gatataagtt tgaactttat actaaatttt tagaattttc aatcaagaca ataacataat
     5941 agctaagcaa ttagaacaca actaagatgt ataaggcatg tatatgatga atgggaggat
     6001 ttaattaatg ttattagaga aattaagaaa agaagtatta caagcatctt tagacctttt
     6061 aaactataat ttagtgacat taacaggagg gaatgtaagt gggagagatg aacaaactgg
     6121 ctatatagca atcaccccaa gtggaatgga ttatagaaat cttacaccaa gtgatattgt
     6181 tatagtagat gtagatggaa atattataga tggaaaatgg aaagcatcag tggatttatc
     6241 agaccacttg tatatatata agcatagaga agacataaat agtataattc atacacattc
     6301 tacatactca agttgttttg ctattttaaa tgaacctata gaatgcgcaa gtacaactct
     6361 tgcaaatgaa gtagggggtt cagtaccagt tgcaaaattt tcaccaccaa catttaaaaa
     6421 gatgggaaaa tgtgttattg aagcaattgg tgacaaaaga gcatgtcttt tagcaaatca
     6481 tggcgtaatt gcagttggtc catctgtagg acatgctctt acagccgctg taatgttaga
     6541 agattcagca aaagtatatt acttagcaaa atcaattggc acaccagtgc tactgcctga
     6601 tgaagaaatt caaagagcga gagatgtatt ttttaatgtc tatggtcagg ataaataaat
     6661 tagataaaat tttaaaagta atatgaaaat atagatattc aatgatatta aggagattta
     6721 taatgatata ataagtttaa tagttaagaa gtaaaatatg aaatgtaata gtatataaat
     6781 tcatttaaga ctaaaaataa taagataaaa taaaaaggag tctgtatttc tatgtattac
     6841 ttattaaaca aaagcacaaa tccttacttt aatttggcat tagaagaata tctattttta
     6901 aatgataaat ataatgatga tattataatc atatggagaa atgaagactc catttttata
     6961 ggtaaaaatc aaaatccata tcaggaagta catcatgatg ttattgaaaa gggagaaatt
     7021 cctattttaa gaagaatatc tggagggggc actgtttatc atgatttagg taatatcaat
     7081 atgtcattca tacaaaaaga taggcaactt catgagatag attttttgga gcatactaag
     7141 tttatgcaaa agatgctttc aaccttagga ctagatgtat ctataactga aagaaaagat
     7201 ttatttttaa atgggaaaaa aatttcaggc tctgcacagt ctataaaaag aaaaaattct
     7261 ctttatcatg gaactcttct ttacgattca gatttaaata aattaactaa atatttaaat
     7321 tcaaataaag caactgaatc aaatgcaact aaatctgttt caagtaaagt tacaaatatt
     7381 aagtttttac tcgaaaaaga tataaattac tttttagatt attgtgttga atatttaaaa
     7441 tctaatataa agaatataaa agaacttgaa ttggatgaag aagatattaa aagtgtatac
     7501 aatataggag aagataaata tagaaagata gagtggacat atggtaaaac gccgaaattt
     7561 caagtagtgc taccatttaa taatgaaagt aaaataaata tacatgttaa cagatggaaa
     7621 gtatcaaaat tttttatttc ttatgatgat aatgtaatta atatggataa gttgttagat
     7681 ttgaatttct ttaaagaaga aataaaagag agtattttag aaaattatcc acattacata
     7741 gatttaatca atttaatatt ttaatataaa acctctaatt tgataaaatt gtcttatatc
     7801 aaactagagg tttttaatat tcttgcatat taacaaccat tacaaaattg taaaattaaa
     7861 aacaacacta ttgaaaaatc aatatatatt gtaataaata tatgtgtttt tcattagcaa
     7921 atagacatta actatttata caactgaaaa tttaatatat aattaatcag atggttaaaa
     7981 taaaaatatt aaaagtttta ataaatgatg gttaaaaata aaatacgagg tgataccatg
     8041 tacagtgtta taattgttgc agcgggaagc ggaaggagaa tgaacttaga tataaacaaa
     8101 cagttcataa aattaagaga aaaagaaatt attgctcata caattcaagt tttttatgaa
     8161 aatataaata tagatgaaat agtggtatgt ataaaaaaag aagaagagga tttttttaaa
     8221 gaaaatataa taaataagta taactttaaa aatataaaaa tagcatatgg cggaaaagaa
     8281 agacaggact caatatataa tggtttaaaa aaattagatg aaaattgtga tatagtactt
     8341 atccatgatg gagcaagacc atttgtcgac catagaataa tcaacgaatc tataaaggta
     8401 gctaaagaaa aaaaggctgt ggttgtaggt gttccagtaa gtgatactat aaagatagtg
     8461 tcagatggaa cagtacaaga aactccagaa agaaatttgt tgtgggcagc acaaacccct
     8521 caaacctttg aatataatct cataatagat gcttatgagc aagcatataa gaacaattat
     8581 tatggtactg atgattcaat gttggtagaa aatataggtc aaagtgtcac tatggtaatg
     8641 ggttcttatg aaaacataaa aataactagt ccagaagatt taaatatagc agaacaaata
     8701 ttaaatatgg aaaaaagaga tgaagttaac agtagaagga gaattatatg agaataggat
     8761 taggatatga tgtacacaaa ttaacagaga atagaaaact tataatcggt ggtgtagaga
     8821 taccacacga taaaggtttg ttgggtcatt ctgatgctga tgtattgata catgcaataa
     8881 tggattctat tttaggtgct ctagcattag gagatattgg gaagcatttc ccagatacag
     8941 atgaagaata taagggagca gatagtatga aattattaga acatgtgtat aatctaataa
     9001 caagtaaagg atataaaatt ggtaatatag atagtactat aattgctcaa agccctaaga
     9061 tggctccata tattgagagt atgagaagta atatatcaaa agttttaaac acagacattg
     9121 ataatataaa cataaaggca acaacagaag aaggtcttgg ttttacagga gcaaaacagg
     9181 gaatagcttc acaaagtatt tgtttattat tgttgacaag tcagaataat taatatatta
     9241 ttagtaataa gtttaatgga tatcaagtaa taggcctata tagaatatct ataatgtatg
     9301 aatttaagtg aaattaatat aaatataaaa ttagctatga taaagaaata gtaaaatgat
     9361 gaaatcttca cagagagaaa acggtggtga gagttttcaa gaggaagtct ttgaacctgt
     9421 cttttagctt aaaggtataa aaatctttac ggtaggaacc gttatattcc caaaagagag
     9481 ctattcatat agctaactag agtggtaccg cggaaattaa acctttcgtc tctatacttg
     9541 tatagagatg agaggttttt tatattttca ggaagttaat attcaggagg aatctattaa
     9601 tgaaaatgtc aaaaatgttt atgccgacat taaaagaaat cccagcagat gctgagatta
     9661 caagccatca attaatggtt aggtcaggca tgataaaaaa aatgacttca ggtgtttata
     9721 accaattacc aatggggtta agagtgttca aaaaaattga acaaataatt agagaagaat
     9781 taaataaaaa agattgtcaa gaaatacttt gtgcagcctt acttccttct gaattatgga
     9841 aagagtctgg aaggtggact gctatgggtg aagaaatgtt tagactaaaa gacagaacag
     9901 aaagagagta ctgtcttggg ccaacacatg aagaagcatt cactgatata ataagacaag
     9961 aaattacttc atataaacaa ttgccactga acttgtacca aatacaagta aaatatagag
    10021 acgagagaag accaagattt ggtgttatga gaactaagac ttttacaatg aaggatgcat
    10081 atagttttga tgtagatgat aaaggtttag ataagtcata tcaagatatg tttgatgcat
    10141 atgtaagtat atttgataga tgtggattgg agaatagccc tgtacaggca gattcaggag
    10201 ctataggagg ttctacatca gccgaattta tggttaagtc agaagttggt gaagatgaag
    10261 ttgtattttg tagtggatgt gattatgcag ccaatgtaga aagagcagaa tcttgtaatc
    10321 tagcatcaca aaaagaagaa atgaaagaat tagaagaggt tcatacacca ggagcagcta
    10381 caataaaaga gttagaagaa tttttaaaaa cttctccaga taagtttgct aaaactttag
    10441 tatatgaggc agatggaaaa acagttgtag tcgttgtaag aggagataga gaagttaatg
    10501 aaatcaaagt atctaatgct ataggttctg ttatagaatt tgctcttgct acagatgatg
    10561 tagtgagaaa agtaactaat gcagaagtag gatttgcagg tccaataggt ataaatgctg
    10621 actatgtatt tatagataaa gaaattgttg aacaaagaaa catagttgtt ggggctaata
    10681 aaactgagta ccatataaaa aatgctaact atggaagaga ttttgaagga atagttggag
    10741 attttagaaa tgttcaagaa ggcgaccagt gtatagtatg tggtaagcca cttgaaatag
    10801 ctagaggtgt tgaagtagga catatattta aactaggaac taagtactca gaatctatga
    10861 atgcaaactt catagacaaa gatggaaaat caaagcctat agtaatggga tgttatggaa
    10921 taggtgttga aagaacagca gcagctataa ttgaacaaca taatgatgag aaaggtataa
    10981 tatggccttt gtcagtagct ccataccatg ttgtgatagt tccagcaaat atgaaaaatg
    11041 aagaacaaat atcaatagct gaaaatatat ataatgattt gcaagctatg ggtgtagaag
    11101 ttttattaga tgacagagat gaaagaatag gtgtcaaatt caatgattca gaattaatag
    11161 gaatacctat gaggattaca gtaggtaaaa atattaatga aggtaaagta gaatttaaac
    11221 ttagacataa agaagataaa gagataattg acatagaaga aataaatgaa aaagtaaagg
    11281 cagagtttat caggaataat gttaggttag gataataact taaataagat attgtataga
    11341 taatcaaagt tgtactttat agacaccatt ttatctatat ataaggatat aaaatggtgt
    11401 ttttttataa aacaaataaa tgaggaggta attgaaatgg caaaaaatga aaaacaattt
    11461 gttgaagaaa taacaaagat ggaagatgat tttccacaat ggtacacaga tgtaataact
    11521 aagactgact tagttgatta tgcaccagta aaaggtttta tggttgtaaa gccatatgga
    11581 tatgcattat gggaaaagat gcaagaattt atggataaaa aatttaaaga aactggacat
    11641 aaaaactgtt attttccatt attaatacca gagagtttat taaacaaaga agcagaacac
    11701 gttgaaggtt ttgctccaga agttgcatgg gtaacacatg gaggaaataa aaaattagaa
    11761 gaaagattat gtgtaagacc tacttcagag actataatat gtacaatgta tgcgaaatgg
    11821 ttaaaatcat atagagaatt accatatctt tataatcaat ggtgctcagt tgttagatgg
    11881 gaaaaatcaa ctagaccttt cttaagaaca tctgagtttt tatggcaaga aggtcataca
    11941 ttacatgaaa cagcagaaga agcacaagaa gaaacaatac aacaattaga agtatataaa
    12001 gcgttatgtg aagaattact tgcaatgcca gttgtagctg gtcaaaaaag tgaaagtgag
    12061 aaatttgcag gtggtgaaag aacatatact atagaagcta tgatgcatga tggtaaagca
    12121 ttacaatcag ggacaagtca cttcctaggt caacacttta ctaaggcttt tgatattact
    12181 tttgcagata gagaaggaaa tttagctaat ccataccata catcttgggg agcatctaca
    12241 agacttatag gtggacttat aatgactcat agtgataaca gaggtttagt attaccacca
    12301 agagtagctc caattcaagt tgttatagtt cctatagctg ctaaaaaagg aaatgtaatg
    12361 gagactgttg ataaaatata tgctgattta aaaactaagg gagtagccgt agaagttgat
    12421 gatagagata actatacaac tggatggaaa tttaatgagt gggaaatgaa aggtgctcca
    12481 gtaagagttg aaattggacc aaaagatata gaaaataatc aagctatggt atttagaaga
    12541 gatactttag aaaaggattc tatgccttta gaaggattag ctgatgctat atgtgattta
    12601 tttgatgtta tacacaatga tatgtttgaa aaagcaagaa aacatagaga agacaataca
    12661 tctatagttg aaaatatgga tgagtttaga aaagctttag aagaaaagcc aggattcata
    12721 aagactatgt ggtgtggaga tgcagaatgt gaagctaaga ttaaggaaga aacaggagca
    12781 actataagat gcttaccttt tgaacaagaa aacttaggtc acaagtgtgt gtactgtgga
    12841 aaagaagcag atagtatggt agtaatggca aaagcttact aaaatacatt taaataaaaa
    12901 ctggggtaga aagtatttaa tactttatcc cagttttttt gttttttatg agtaataaaa
    12961 tgtatttgta actatgtcat aagataagtc atagatactt atattaaaaa gaatataata
    13021 gagaactatt tttaataatt acatattaat gaaagcaaaa tatttttgtt atatagattt
    13081 tcaataaagc aaacaataaa tataaatgaa tattagtaag gagtgctttt aatttttaga
    13141 aaataaatga cttaaaagaa agatttatga aattaaaact aggattttct gatataataa
    13201 acatgtgtat ttttaaataa aaaggatatt attattagat ttgattaaaa ataaacttgc
    13261 aaaacaatgt actagtttaa tattatagtt atattgaata aaatatcagg aggaagtaaa
    13321 aatgaatgta agagtaaggt ttgcaccaag tccaacagga tttgtacata taggtagttt
    13381 gagaactgct ttatataact atttatttgc aaaaaaaatg ggtggagaat atatattaag
    13441 agtggaagat acagaccaaa gtagattagt agaaggtgct atagagaata tgcttaatgc
    13501 tatgaaatgg gctggtgtca accatagtga aggtgttata ttagatgata gtggaaaagt
    13561 agtacaaaaa ggagaatatg gtccatatat tcaatctcaa agattagata tataccaaga
    13621 atatataaag caacttttag atagtggaaa agcttattat tgtttctgta caaaagaaag
    13681 actggatgaa gtaagagatg ctcaaagagc agcaggagaa acagctaaat atgatggtca
    13741 ttgtaagaat ctttctaaag aagaagtaga agcgaatata aaagcaggaa taccatatgt
    13801 tataagatta agattgccag agaatcatac aattaagttt acagacttag ttagaggaga
    13861 tatggaattt aatactaatg acctagatga ccaagtatta atgaaaacag atggatttcc
    13921 aacttatcat tttgcagtag ttgttgatga ctatttgatg aaaattacac atgttataag
    13981 aggagaagaa tgggtatcat ctactccaaa acatgtttat ttatatgaag cttttggatg
    14041 ggaagcacct gtatttgtgc atttaccaaa tatacttaat aaagaaaaga aaaaattaag
    14101 taaaagacaa ggtgatgtag ctgttgaaga ctttaagaaa aaaggatatt taccagaggg
    14161 attagtaaac tatgtagcat tagttggttg gtctcctgaa gataatcaag aattgtttac
    14221 aatggaggaa ttagaaaaag ccttctctgt agaaagagta tctaaaagtg gtggagtatt
    14281 tgatactgaa aaattaaatt gggttaatca gcactatata aaagatggtg atgatgctta
    14341 tctaacagac ttagcaatac catttttagt agaagatgga tttataacag aagaagaagc
    14401 tactaataaa tatgatttct taaagagtat gatatcagta cttaaagaga aattacaata
    14461 tgtaaaagaa attactgaac atgcaagtat attttttgga gataaaatag aggtagaaac
    14521 tgaagaggga agagagttct taagattaga acatatacct actttaatag atgctttgag
    14581 agaaaaaata gaaaaaacag aggtattaaa tgctgagttt gttcaagcta tgcttaaaga
    14641 aatacaaaaa gagtacaaaa taaaagggaa aaatttattt atgggctcaa gaataatttt
    14701 gacagggcaa atgcatggtc cagatttacc aaaagtaatg gaagttttag gaaaagaaac
    14761 ttgcttaaat agaatagctt atgtaaaaaa taatatatta tagtcataaa agtccgctaa
    14821 tttagaagcg gacttttttt gtgttagtat ttggtgaaaa tatctgctta agaaaatgaa
    14881 ggtatttaag gtaataaatt taatatataa aaagcaaagc aaaaatataa ataaaatatg
    14941 aataataaac taataaaata ttaagcatag aagaagggaa tctattattt tagtttgatt
    15001 taattattaa gggggtaatt actatgtata gtaatattga tgatgttaaa aaggaattaa
    15061 aggagctatg cttagaatat gttacaattt tagaaaaatt aaaagatgaa aaaatgatta
    15121 cagaagaaac ttttgaaaag tgctcttctc aaaaaaaaat atttttagaa gaacaataga
    15181 ggatggtaat agtattatta ttatgtagtt tgaaagatag tacgaaaaat gatagtaata
    15241 ctattattta ttgcaatttt caactataat ataatatata atgttaagaa gataatttat
    15301 aaaaatacag tgaggtgatg agtgtgaaag tttataatac attgactaga acaaaagaag
    15361 aatttgttcc attagaagaa ggaaaggtaa aaatgtatgt gtgtggtcca acagtttata
    15421 actatataca cataggtaat gcaagaccat ttataatatt tgatactcta agaaggtatt
    15481 tagagtatag aggatacgat gtaacttatg tacaaaactt tacagatgtt gatgataaga
    15541 ttataaatag aagtcatgag gagggaatat ctcctgaaga agttgcggct aagtatataa
    15601 aagaatattt tgtagactgt gatgggctag ggataaaaag agctactgtt catcctcagg
    15661 ttacagataa tatacaacag attatagagt ttataaaaga gttggaagat aagggatatg
    15721 catatgcagt aaatggtgat gtatattttg atacaaataa atttgaaggt tatggaaaac
    15781 tttcaggtca aaaacaagaa gatttagagg caggggctag aattgaggtt aatgaccaaa
    15841 aaagacatcc tatggacttt gttctttgga aggcaaaaaa agaaggtgag ccaggatggg
    15901 atagcccttg gggagaaggt agaccaggat ggcatataga atgttctgtt atgtctaaga
    15961 gatatttagg tgaaacaatt gatatacatg caggtgggca agatttaaca ttcccacatc
    16021 atgaaaatga aatagctcaa agtgaagcta gaagtggaaa aatattctca aaatactgga
    16081 tgcataatgg atatataaat ataaatgatg aaaagatgag taaatcaaaa ggtaatttct
    16141 ttacagtaag agatatatca aaattatatg atttagagat agtaagattt ttcatgttat
    16201 cagcacatta tagaaatcca gtaaacttta gtgatgagat gttaaatcaa gctaaagcag
    16261 gtcttgaaag actatataat actaaagaaa aattagaatt tacgctaagt aatttagtag
    16321 aatctccttt aacagaaaaa gaagtagagc ttgttaaaga attagatgac tttagacaaa
    16381 aattcataga tgctatggat gatgatgtta atacagcaga cgcagtaagt gtaatatttg
    16441 aattagctaa gttaataaat tctaatgtag atgaaaattc ttctttggag tttgctaaaa
    16501 aatgcttaga tgaatttaat gaacttacag gagtattaaa tattgtaaat aagaaaaagg
    16561 atactgtatt agataaagac attgaagaat tgatacaaaa aagaactgat gcaaagaaaa
    16621 ataaagagtt tcaattagct gatgatatta gacaacaatt actagatatg ggaattgtat
    16681 tagaagacac aagacaaggt gttaagtgga aaaggatata aaaatggaaa aaactgaatt
    16741 agttactatg tcaccattag ttttagcata cttaggtgat acagtgtatg agacatatat
    16801 aagagaatat ttaattagac agaatacaca aagaaaagtt aatgatttgc ataaactagc
    16861 tataaagtat gtaaaagcta aagctcaggc aactataatt catgaaattg aaattgagtt
    16921 aactgaagaa gagagtaaaa tttataaaag gggaagaaat caaaagtcaa atacttctcc
    16981 taagaatgca gatattatag actacaaaca tgcaactggg tttgaggcat tagtgggata
    17041 tttgtattta aataatgaaa tagagagact tcaatatatt atcaataaag gaatcaaaat
    17101 tattgaaaga gatatgtaaa tattaaagag tgttgtctta ataataagta ataaggcaac
    17161 acttttttta taactaatgt ataataaata tatagaaatt agtaatgaga aaggataaga
    17221 aagttatgaa agtaaaatta atatcacata cacctgaacc agaaaaagta atagctatgg
    17281 cagctaagct atgttattca ccagttggga cagacgaaat agaaaaagat ttctaaaata
    17341 tattaaagag cattcctata acaaatacta tggcggttgg aatctatgag aagttataga
    17401 tatagaatta tgtaaattat ttggaaaaaa caggtttgaa aattagccat gttggtattt
    17461 ctaggttact taaggaagat caaacataaa taagctgagt tgtcttaggt gcactatgta
    17521 caacattaga atgtgaagtt gaagatttat tacttcaaac gaacgatggt actaagaaaa
    17581 acaatgatga aatgattaat taataataaa ggtaaggagg tagtatatga gtttagcaga
    17641 taaaattttt attgatatgt gtaataatat tcttgaaaat ggagtatcat cagatggaga
    17701 ggtagttaga gcaaaatggg atgatggcca gcctgctcat acaataaaga agttttgtgt
    17761 agtaaatagg tatgatcttt caaaagaatt cccaatactt acactaagac caacaaactt
    17821 aaaagcagca atagatgaat tactttggat atggcaaaaa aaatctaata atataaatga
    17881 tttgaatagc cgtatttggg atagctgggc tgatgaaaca ggttcaatag ggaaagctta
    17941 tggataccaa attggacaga aacataaata caaagaagga gaatttgacc aaatagatag
    18001 agtgatttat gacttgaaat ataatcctta tagtaggaga attataacta atatatataa
    18061 tcataatgat ttaaatgaaa tgaatttata tccttgtgca tatagtatga catttaatgt
    18121 aactggaaat agattgaatg caatattaaa tcaaagatca aatgatattt tggtagctaa
    18181 taattggaat gtaactcagt atgcaatttt agttcacatg ttagcacaaa taagtgattt
    18241 ggaagtaggc gaattggttc atgttatagc ggatgcacat atatatgata gacatattcc
    18301 tctagttaaa gagcttatta atagagaatc ttatgatgca ccaaaattaa taataaatcc
    18361 aaacataaag aatttctatg actttagagt tgaggacttt acattagaaa attataaaag
    18421 tgggccacaa atcaaaaaga ttcctgtggc aatttagaag gagttattta tgaaagttat
    18481 agtagctgta gatttaaatt ggggaattgg ttatggtgga aagttactac agtcgattcc
    18541 tgaagatatg aaatttttta aagaaaaaac tgtaggtaat gtagttgtaa tggggagaga
    18601 aacctttaaa tcattaccag gaaaaaaagc attaaaagat agagtgaata tagtcttgac
    18661 tagaagtaaa tcttttaatg atgatagatt gattgtttgt aattctatag atgaaactct
    18721 taaggaacta caaaaatatg aaaatgataa aatttttata ataggtggag aaacagtata
    18781 taaacaattt ttaccttatt gtaatgaact atatgtaaca aaaatacaga agacatatga
    18841 ggcggataga tttttcccta atatagatat tatgtcaaac tgggatttga ttaaagaaag
    18901 tgaagtaaaa gaatataaca atataagata tgttttttca acatatataa ataatagttt
    18961 ataggtttgt ataggagaga aatttaattt atgaaaaagg atatatttgt aaatagagtt
    19021 tctaatgctt tagaaaatga tagaggcgca gtttttgtag gtagtggtat atcgtttagt
    19081 tcgactgaag ttgattggtt taaattactg gagccattag taaatgaact tgatataaca
    19141 ttagacaata aaagtgatga tttacctatg attgctcagt acatagtaaa taattattca
    19201 ggaaatagag ggcctttagt aaacgaaata tcaaaaacat ttaatagaaa gtttaaacta
    19261 aacaagtatc ataagtattt agcaacaact aaactttcaa caatatggac aactaattat
    19321 gatatgttgt tagaagatgc attttatgat tttgatacat cggttaaagt aagtgatgat
    19381 tctattacaa ggaatagact agaatatgat gtggaaatta taaaaatgca tggatgtatt
    19441 tctagatccg attggaatga aatagttata actcaagaag attatgatga tttttcaata
    19501 aataaaccag ctatatctca aagattaagt gaagatttaa taaataagtc ttttctattt
    19561 attggatata gctatagaga tccaaatata agaaatataa tgataaatgc tagaaggcta
    19621 agtaaaaaac atactcaaca acattatata attttaaaaa agttaaaaga tagtgatagt
    19681 aatattacgc aagatgatgt agtaagacaa gaactttggt gcaatgattt aaaacgtttg
    19741 ggaatttcaa ctttatttat agatgaatac tcagagttag aggaactttt aagagagata
    19801 agtcaaaaat caagaggtaa aacagtatat gtgacaggtt ctcataaaga aagcaatatg
    19861 aatgcaagaa aattaggaac caagctagca aaacataaag atattacttt actaagtggt
    19921 caatcatctg gtattggctc agaggtagtt gctgcgttta ctgaagagtg tattaagagt
    19981 aaagaagata ttataaatag aatgaaaata tttcctaatc catattcagc tgatgaaaaa
    20041 tactctgatg atattacttt aataccagat ttaaagaaat gtagaactaa aatgttgaat
    20101 tcaacacaag ttgtagttgt atttagtgga ggtataggta ctgaagcaga gatagatgtt
    20161 gctataaaac aaaactgtaa tataattcct gttataacat gtaaagagga tagagagaat
    20221 ccagctataa aaaaaatctt aaactatagt gataatatga aaaatataga aataaaagat
    20281 gctacctatt ataataaaat agtagatgaa gatggtgttg taacaatgga ggaaatttat
    20341 atatgcatat caaaaataat gaattaaaac atgacattat taaattgaaa aaaagaaatg
    20401 gatttattga agttggaaaa gataaaaaaa cgttggtaaa ctgtttaatt ggagcgaatg
    20461 aattagagga ttacaaatat gaaataaaaa aaatagattg tatacttaat atatcagaaa
    20521 aaccagatat agtttcagat ttaagtacta aaagaagaaa aataagtgaa tctatatggt
    20581 ataagatagt taaagataca cctttcgtat ctgctacact gcccatatac ttagttagta
    20641 aaaataataa taaaatagat agtaatgaat tattagatat aattattgag cagatggaaa
    20701 atggagttgg gatgataacg attcatccaa cagcaaataa acaaattttt gaggattcta
    20761 agaatagaat ggtacctgtc acatctagag gtggaggtat ggtaatacaa gatttaatct
    20821 caaaaaattt ttcagaagat aatgtgtatt tgaaaatatt gccagaaatt ataaagtatg
    20881 ctcaaaaaca taatgtttgt atcagtctag gtgcaacatt tagatcagga aacatatttg
    20941 attctaatga tagagcacag ataacagaaa ttaagagtca aatagaatta gctaagagga
    21001 tatataaaag taatgtcggg gttattattg aatcaccagg tcattcaaga ccaagtgaca
    21061 taattaaaat atcgtcaata ttaaaaaaag aaggtttccc aattatgcca ctaggaccaa
    21121 taccaacaga tattgcaata ggaatggatc atatcgctgc agctattggt gctacaatta
    21181 tgggtataga aggatgtgca aatataatat caactgtcac acgagaagaa catactggag
    21241 ggagaccttc tattgaatcc acagttgaat ctataagaac agcaaaaata gctgctcata
    21301 ttattgatat acatcaaatg aatgatatta acttagatat gagtgtatct aagtctagag
    21361 ctaaatctaa cacctgtata tttgggaaag atacaagata ttgtgacaga tgtagagaac
    21421 tgtgtccttt gtgcattaga taaaaattat attcaagtta attcaataaa aaagattgga
    21481 taaaaataca tcgtatagct agtttttcac aacagagtca aagatatgtc aaattagagc
    21541 aatttgaata cataattcct ccagaaatag aaaaaataga aaaagcaaaa gagttattta
    21601 taaattcaat gaaaaaatac caagaggatt atgataaatt agtagagata ctatttgaaa
    21661 atcattataa caaactaata aaaggtggta aaaacgagaa aacatcaaaa agacaagttg
    21721 agaagaaagc aatagaagat gctagatacg tatttccaaa tgcgtgtgaa acaaagatgg
    21781 tatttactat aaatgctaga agtttgttta atttctttga acatagatgt tatgaaaatg
    21841 ggagttgtct agaaggaact atgacttgtg gagatatagt tcaagttaga aaaaaattta
    21901 agaacttgta ggaggtgaat aatttggcga gtatagaagg aagaaatccg gtaatagaag
    21961 caataaaaag tgacagagaa atagataaaa tattaattgc aaattcagct aaagaaggtt
    22021 caattaagaa aataatagga atggctaaag ataagaatat aattattcag tatgttgata
    22081 aacataagct agatgaagta agtacaagtc attcacatca aggtgtaata gcttatgcaa
    22141 gtgagtataa gtattatgaa ttagatgagc taatagattt agcaaagaat aaagatgaag
    22201 acccattttt cattatatta gatgagataa cagacccaca taatttgggt tcaataataa
    22261 ggacagcaga tgcagtagga gcacatggag ttatcattcc aaaaagaagg tctgtacaca
    22321 taactcctgt agttgccaaa gcatctgctg gagcagtaga atatatgcca gtttgcaagg
    22381 taaccaatat agttaataca ataaaaagac tcaaagaaga agggctgtgg attgctgcag
    22441 ctgatatgga tggagaaact ttttataaac aaaatcttac tggtcctctt ggtgtagtaa
    22501 ttggaagtga aggttttgga atatctagat tggttaaaca aaactgtgac tttatagtaa
    22561 aaatgccaat gataggtaat gttacatctt taaatgcatc tgtagcaggt ggaatacttt
    22621 tatatgagat attcagacaa agactggata aaagtaaata ggcatgaaaa aaaatataaa
    22681 tcattactta ataatagatg gctacaatat tataaatgcg tgggacaatt tgaaagaact
    22741 ggctaaagag gacttagaag attctcgaga aaagcttatt gatgacatta tagaattttc
    22801 tgagtttatg ggttataaga ctataatagt ttttgatgct tataatgtta aaaattctcg
    22861 agaaaaagtt gaaaaaagaa aacacataac aatagtttat acaagagaac accaaacagc
    22921 agatagttat atagagaaat ttataacttc tttatcaaag tatgatgatg taaaagtagc
    22981 cactaatgac tatgcagaac agcaaatgat tttaggaaaa ggtgctacta gaatgtctgc
    23041 aagagaatta aagttagagt tagatcgttc caagaataaa atgaaggaaa aaaatatcgg
    23101 tttgaggaaa aaaattcagc gaaattggtt ggaggaaagg ttagataaag aaacattgtc
    23161 gaaacttgag aacattcgta gaaagcgttg aaattactag gttctttaga ctataatata
    23221 cctataaatt tgttttatat atgggggaga tttacatgtt ggtagcaaaa gaaaaaagtt
    23281 atgagttagt ggacaattgt cagcaggatg agtataacat tgtattaaga gcaagtgaag
    23341 gggataaaat agcactagag tacattatta ctaaatacag aaattttgta aaggcaaaag
    23401 caaagtcata ttttttaatt ggagcagata aagaagatat aatacaagaa ggaatgatag
    23461 gcctttataa ggcagttaga gattttgatg gaagtaagac caattcattt aaatgttttg
    23521 cggagatatg tataacaaga caaataataa ctgctataaa aactgcaaca agacaaaaac
    23581 atataccttt aaattcatat gtttctttga ataaaccaat atatgatgaa gagtcagaca
    23641 gaacactttt agatattata gctacaagta tagtaacaga tccagaagag cttataatta
    23701 gtaaagaaga gttaaaaaat atagaatcaa aaatgaacga attgttaagt gacttggaat
    23761 tagaagtact agaattatat ttaaatggaa agtcatacca gtttatagcg gacaagctta
    23821 aaagagatgt aaagtcaata gataacgctt tacaaagggt taagagaaaa ttagaaaaac
    23881 atcttgaaaa cagaaatgat taatagtaaa atacttacca taagcttatt gtgagcaaaa
    23941 ttaatttata tcgaaagata agggaggaaa ttcaaaatgg ctaaagctaa atacgaaaga
    24001 acaaaacctc atgttaatat agggacaata ggacacgtag accacggtaa aactacatta
    24061 acagcagcaa taacaaaaac attatatgac agatatcaat taggagaagc agtagatttc
    24121 gcaaacatag ataaagctcc agaagaaaga gaaagaggaa tcacaatatc aacagcacac
    24181 gttgagtatg aaacaccaaa tagacactat gcacacgttg actgcccagg acatgctgac
    24241 tacgttaaga acatgataac aggagcagca caaatggacg gagcaatatt agtttgttca
    24301 gcaacagatg gaccaatgcc acaaacaaga gagcatatac tattatcaag acaagttgga
    24361 gtaccata
//
LOCUS       DAMJWP010000039        22908 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.040, whole genome shotgun sequence.
ACCESSION   DAMJWP010000039 DAMJWP010000000
VERSION     DAMJWP010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22908
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.040"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            235..699
                     /locus_tag="PC700_001331"
     CDS             235..699
                     /locus_tag="PC700_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HDF2580646.1"
                     /translation="MATMTDIIEKFIKDLMEEDNSIQIQRNELANLFSCAPSQINYVL
                     TTRFTIDRGYYIESKKGGGGYVQIEKIRKSKDGHIRELLNEKIGSQISYKKAKELLEG
                     LKESDLINERELKLILYAIDDKSLCMPIYELKEKVRSNILKNIIIGLFSIEE"
     gene            706..1200
                     /locus_tag="PC700_001332"
     CDS             706..1200
                     /locus_tag="PC700_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HDF2580647.1"
                     /translation="MLCQKCNKNKASVYYNKIVNGEKTEMYLCSECAKENTEMNFNLD
                     MPFSMMDIFSNLGFQPKKELEEKLVCPKCNTTYSEFKNNGRFGCSECYNAFSSQVNPM
                     LQNIHGHIEHTGKAPKKSFYKISVENEIKELKEDLDRAIKNEEYELAAQFRDKIKYLK
                     GSID"
     gene            1216..2241
                     /locus_tag="PC700_001333"
     CDS             1216..2241
                     /locus_tag="PC700_001333"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HDF2580648.1"
                     /translation="MKENIVMKSRVRLARNLNNYPFPNKLDKECAMEIIEKVKNAFIN
                     SNLEQKEEFDFYKIEDLDQSKKMLMVEEHIISPDLAENDKSAVIVKKDKTISIMINEE
                     DHIRIQTICDDLNLEYAYSVANEIDDLLESSLEYAFNTKLGYLTSCPTNTGTGMRASV
                     MMHLPALSQLGYMDELYKISSQIGIAIRGIYGERTEALGNIYQISNQLTLGRTESNII
                     ENVSGLTKDAISKEIKAREILQKKLGIKLEDKIFRSIGTLENSRVMSSAEAMSHLSNI
                     KMGIEMNYIDKLDLRAIEQLMIGIQPAHQSIMYKSDDVENRDINRATYIRETLEKLRG
                     GMNCELQ"
     gene            2228..4675
                     /locus_tag="PC700_001334"
     CDS             2228..4675
                     /locus_tag="PC700_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDF2580649.1"
                     /translation="MNFNRFTQRAKKAIDLAFESAKSLGHNIVGSEHILLGLLREEEG
                     IAAKVLSKVGFTEAYLEGKIVDMEGKGEEISEDIVLSPRSKQILELSGMFANKLKTNY
                     IGTEHILLAIIQEGEGIANKILNYAGVNDRTLAQLTIDMMGISDKNQYKAENSYTGNQ
                     NQAESKVLDKYGRNLTLYAKQNKIDPVIGREKEIQRVIQILSRRTKNNPVLIGDPGVG
                     KTAIAEGLATNIALGNVPETLKNKTLYSLEMGSLLAGAKYRGEFEERIKEVVDEVVKN
                     GNIILFIDEMHTIIGAGSTGEGSIDASNILKPALARGEIQVIGATTIDEYRKHVEKDS
                     ALERRFQPVMVDEPTKEDSIKILEGLRDKYEAHHKVKITDDAIKAAVELSTRYISDRY
                     LPDKAIDLIDEAASKVRLKENTPPSEIKKLELEIENIDKEKEEAVRCQDFEKAAKIRD
                     EQGLLKKQLEDVRERWNKSSKHSDLVDGEVIAEVVGLWTGIPVNKILEEEADRLLRLE
                     EILHNRVIGQEQAVKSISKAIRRSRAGLKDPNRPIGSFLFLGPTGVGKTELSKALAEV
                     QFGDENQIIRIDMSEYMEKHAVSRMIGSPPGYVGHDEGGQLTEKVRRNPYSVILFDEI
                     EKAHPDVFNILLQILDDGRLTDSKGRTVDFKNTIVIMTSNVGASTIGRQKTLGFSIAK
                     GDEEEKSQYEKMKENIMGELKQRFRPEFLNRIDDIIVFHSLNENHISKIVLLMAAKLQ
                     ERLKEMDIKLEMSDEAVKLISKSGFDLEYGARPLKRALQKELEDELSEAILKGNVKKG
                     SNVVAKVKDEKIVFETK"
     gene            4855..6228
                     /gene="radA"
                     /locus_tag="PC700_001335"
     CDS             4855..6228
                     /gene="radA"
                     /locus_tag="PC700_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDF2580650.1"
                     /translation="MAKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKK
                     EVFIIDKSSSKPVSINSIESKEEERFTTDINELDRVLGGGIVKGSLVLVGGDPGIGKS
                     TLLIQVSSNVANLGKTVLYITGEESESQIKMRAKRLGINSENLYIFAENNLSIIESYL
                     ESVNPELIILDSIQTVFSPEISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKE
                     GSLAGPKLLEHMVDTVLYFEGERYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDN
                     PSKILISEKPKDVAGSVIISTVEGTRPMLLELQALVSPTSFGIPKRTSTGVDYNRVGM
                     LLAVLEKRVGLQIQNQDVYINIVGGIKINEPSIDLGIAISVASSFRNIPIDENIAVTG
                     EVGLTGEVRAVSFIEKRIAECKKLGFKKIVVPRSNYDVVKETKGIEIWPVDNLRQAIN
                     IVLGRNQ"
     gene            6231..7301
                     /gene="disA"
                     /locus_tag="PC700_001336"
     CDS             6231..7301
                     /gene="disA"
                     /locus_tag="PC700_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA"
                     /protein_id="HDF2580651.1"
                     /translation="MENFLDNKNMLYALKMISPGTPLRLGLNNVLRAKTGGLIVIATN
                     EDVMKIVDGGFAINAEYSPSYLYELAKMDGAIVLSGDVKKILFANAQLIPDYFIETSE
                     TGTRHRTAERVAKQTGAIVIGISQRRNVITVYRGNEKYVVEDISKIFTKANQAIQTLE
                     KYKTVLDQAVTNLNALEFNDLVTIYDVALVMQKMEMVMRVTSIIEKYVIELGDEGTLV
                     SMQLEELMGTTRIDQKLIFKDYNKENTEIKELMKKVKNLNSEELIELVNMAKLLGYSG
                     FSESMDMPIKTRGYRILSKIHRLPTAIIENLVNYFENFQQILDASIEELDEVEGIGEI
                     RATYIKNGLIKMKQLVLLDRHI"
     gene            7390..8484
                     /locus_tag="PC700_001337"
     CDS             7390..8484
                     /locus_tag="PC700_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HDF2580652.1"
                     /translation="MIRKVTRILFILLGFTIGITTYLTLMKDFEILTFGKETYGYIAA
                     VVAGIIIAILGYLIEPWVVNKVKEIAKIVDKELSKYPQTDILLGSMGLIVGFVIAYLL
                     SGLVNRIPIVGGILSLLLYLFLGYLGMKVALKSKNDLFNIGKLGRLANPIKDKDKDKE
                     NKKEVKAIPPKVLDTSVIIDGRIADICKTGFIEGKLIIPAFVLEELRHIADSSDDLKR
                     VRGRRGLDILNIIQKELNIEVEISERDFDDIAEVDSKLLKLAQVLNGKVVTNDYNLNK
                     VAQFQGVEVLNINELANAIKPVAIPGEDMVVQVVKEGKEAQQGVAYLDDGTMIVVDGG
                     RKYMNETIKVLVTSVLQTPAGRMIFAKPKN"
     gene            8613..10790
                     /locus_tag="PC700_001338"
     CDS             8613..10790
                     /locus_tag="PC700_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580653.1"
                     /translation="MRKKIISFLATFIIILTSASQYSFADDMSTRGKVIFIDMNRTSM
                     SNMLRIKSLREELDNRGYIGLMNIRGDKGSDDRRSYASMGAGGRANVANEEDINFESS
                     SKDRNIVFESATGKSAKGINNLTINKSINENLNFGEYGSVLGSLGQSLSENGLKASVL
                     GNSDIIENGQLIKNRNLCLTAMDEYGRIPNGNVDTINKKDLSMPYGISTDYDKLIVET
                     KEAYKNNDVVFVELGDTYRLDLYKPNLNEKTYESVKDNIEKNIDVYLKNIFSMVEEND
                     TVYIASAFPSDLDYKNKRRLSPIIKLNGEGKGILSSSTTRREGIVTNLDVGVEILDNF
                     NIKNQNMVGRKYELINRDDNKEFLMDEYQKIVSISSIRSTILNGFVSIVFLSWIVAMI
                     AILFRKHISKNYKETTFFILKELLKIGIVMPLSFMITPIMNFKTPLAISLGIIIITLT
                     IYLISKVLIKNDLKNMLFFTGLTIVIMVIDAGFGSYLMKSNVMSYDCIIGARYYGVGN
                     EYQGVAIGSAIFTFAILLTYKNIPKWSIIVFSLVILITSASPIMGANVGSAISECVAF
                     LLFILLIYNVKIDFKKIVLLGIAVLFVLGVFVAIDMILGSNSHLGMFVKEIYFNGPGE
                     IIQTFSRKIEMNLKLAQTSAWVNILLTGIGVILVLMINQIRYFKQLMDEYPIVFKGFI
                     ASIAGCLVTLLVNDSGIVSSATAFIYVIVPMITLSINLTALKE"
     gene            complement(10976..11821)
                     /locus_tag="PC700_001339"
     CDS             complement(10976..11821)
                     /locus_tag="PC700_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2580654.1"
                     /translation="MNRNNFSHEIIEPIDNLDVNFKLFDDSGSYVANHWHNSLEIIYI
                     TSGDLQINMEGYTYNLKANECMFINSGIIHSTRCTYQDTSILLQVPLSFLNKYIPDFK
                     NCYFDFKVNANDNNYKKNSGKVKLILENMREIKLSSPPAANLLFTSLLFELLFELYTN
                     FKISVGNKNMKKTVLDLSKFEPVLEYTKINYKMPISINKIAKVAHLQPEYFCRKFKQY
                     MGQTYLEYLNDVRISHIYKDLINTNDTLCSILEAHGFTNTKLFYRIFKEKFKCTPKHI
                     RKKST"
     gene            12038..13204
                     /locus_tag="PC700_001340"
     CDS             12038..13204
                     /locus_tag="PC700_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HDF2580655.1"
                     /translation="MYIKGVNLGGWLVLEKWMTSSLFEGTEAEDEYYLPRQLSREAYE
                     SRIKTHRSEYITERDFATIKSMGFNSVRIPVPYFIFGDCEPFIGCVKELDKAFAWADK
                     YGLSILIDLHTVPGSQNGFDNGGISGICSWSQNPEYVAFTLNVLERLAKRYGMRHELY
                     GIQILNEPITERMWNIMNVPNRFKAVDKEMARGSKPNSLEFLRDFYIKAYKVMRPYMR
                     EENVIVFHDAFELKAWKDFMREEEFKNIVLDTHQYLMLAESDGCEQSIDSYLKYIREN
                     YAKDILQMQKYFPVICGEWSLFNSYACGIDTNGGQSPLNGIESNIDKLSKDDKRELYR
                     KIAKAQLDAWRNGSGHYYWNYKLLLDTVNEEGWIGWDSWDLGKCVAQEWYPIEY"
     gene            13226..14872
                     /locus_tag="PC700_001341"
     CDS             13226..14872
                     /locus_tag="PC700_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDF2580656.1"
                     /translation="MIKNPILPGFNPDPCICRKGDDYYLVVSSFEWFPGIPVYHSKDL
                     KNWELYTHILTDETKIDLKKLPSSKGIWAPCLTYCEEEDLFYIVYGIMNSMNARYFDV
                     DNYLITSKDIKGEWSEPVYIHSSGFDASILHDDDGKKWIASLDWETREGYEKPGVICL
                     VEYCTKKKEIVGYPKRIWSGGTDRGCIEAPHITKRGDYYYIMCAEGGTGYGHSVTMGR
                     AKNIWRPYEKDSMNPIVTSIPGDFYERHDPDHLKPKYYNPESKLQKSGHGSYIETTSG
                     EVYLVHLTSRPFVPELRCTLGRETAIQKMKWTKDNWLRMEDESNLAKEYVSESKLEEH
                     LVSSIPSFDDFDSNVLGLQYYAPRISPLSFADVKSRPGYVRIRGQESRTSLNKVSILA
                     RKLTSVYARITTKMEFYPEVHQHSAGLIMYYDNMNYINLRKYYSETLGQSALSIIHLE
                     NGEKTEFLNTRIPIKDIPIYLRLYIQGRKSYFEWSYDEKNYQRIGKVFDTTKFSDEYC
                     KYGEFTGTFIGLTCADRVKHKHYADFDFFEYIADESKDVD"
     gene            14969..16342
                     /locus_tag="PC700_001342"
     CDS             14969..16342
                     /locus_tag="PC700_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2580657.1"
                     /translation="MNTEVNVKATTIRYEKISFFERISYGCGDLGCNIIYSAMSAFLL
                     FYYTNYADVSAAAVGSIMLVSRILDGFSDLTMGIIVDRTKSKYGKARPWILRMAIPFA
                     IAAVLLFSVPSNLGITSKLIYIFITYNLVSTVIYTAINVPYATLNSLITQNQYERGVL
                     SIFRMILATCGTLLINGLTLPLVEYFGNNLSAWTKTFFVFGIASIMVFFITFTGTKER
                     VKAVKQDRNEVIPFKIGIKSLFRNKYWIQITLCLVCIFIVFAINGGSSVYYAKFILGD
                     EKLFGPINMVSNISQIIAMFMVAPFIKKFGKRNVLIVGSIILISSNVMFIIVGQNYIG
                     VIFASAIKGIGSAGIASTMFAIVSDTIEYGEWKTGYRTEGLINSASSFGFKVGNGLGS
                     AILGAVLSIGGYVGTSATQSDLAIVSIKVCFIYLPIFITILQIIIMFFYKLDKEYSTI
                     LNELNTK"
     gene            16787..17740
                     /locus_tag="PC700_001343"
     CDS             16787..17740
                     /locus_tag="PC700_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HDF2580658.1"
                     /translation="MKKIGDLRLMMKCCSLYYEDNLNQQEIANQLGISRPTISRILKE
                     AFEQGIVKIQIVDVLKNDYQKIERSLERKYKLKEVIVVDDKQDALTQKQELARAVSEY
                     LTRVVKENDIIGVSIGTTLKEIPRYVEKSNCKNVTFIPLLGGIGDNEIDIHANQIAVS
                     LARAFGGDFKLLHAPAVMSDLSTKEKLCKDEKIKEVLDLIDKTTIAIVGIGNPMSLNS
                     TIMASGYMNEADIEDLKKYNSIGAICLQAFDKEGKTSILEFNQRVLGVKLEDLKKIKR
                     TIGVASGDEKIEAIKASLKAKFINSLAINHSLALKLLEDCD"
     gene            17778..18740
                     /locus_tag="PC700_001344"
     CDS             17778..18740
                     /locus_tag="PC700_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HDF2580659.1"
                     /translation="MSISKETLLEMYKRMNQARKFEEKVSWFFARGMVHGTTHLSVGQ
                     EASSVAAVMALEKGDLVSLTHRGHSQFIGMGIDLNKMMAELMGKETGFCKGKGGSMHI
                     ADIESGNLGANGVVGGGLTIAPGAALTQQYKKTGKIVLCSFGDGASNEGTFHEGINLS
                     SIWKLPIIFYCENNLYGMSTSIKRHMNIESIATRAASYGIEGISIDGYNPIEVYETVQ
                     KAAEKCRRGEGPVLIESRTYRWLGHSKSDANVYRTKEEIESWKAKDPIEFLKNYLIEN
                     NLSNEDELDKIQEFAKQSIEDAVEFAQNSPNPKIESLLEDVYAD"
     gene            18765..19751
                     /locus_tag="PC700_001345"
     CDS             18765..19751
                     /locus_tag="PC700_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDF2580660.1"
                     /translation="MSTRELTYAQAIKEAMSEEMRRDENVIFMGEDIGIYGGAFGVSV
                     GMIDEFGPERVRDTPISEAAIAGAAAGAAATGLRPIMEVMFMDFVTISMDAIVNQAAK
                     MRYMFGGKAQVPMVVRCPGGSGTGSAEQHSQSLEAWFCHVPGVKVVAPSTPADAKGLL
                     KAAIRDNNPVIFVENKLLYRKKGFVPEDDYVIEIGKADIKREGTDVTVITYGRMLQSV
                     EEAAETLSKENINVEIIDLRTLYPLDKETIVKSVCKTGRVLICHEAAKTGGLGGEISA
                     LITESESFDYLDAPVKRICGKDVPIPYNPELEKAVVPRVDEIEEAIKSLIVR"
     gene            19764..20810
                     /locus_tag="PC700_001346"
     CDS             19764..20810
                     /locus_tag="PC700_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HDF2580661.1"
                     /translation="MVANKIKATPAARSQARKDNIKLDRLIGSGENGRIHLVDVLNYL
                     KDNKANTTPLARRIAEDLNIDLETIVGTGYSGKIRKCDVEKLTAKETIVSNNTSKYSE
                     KKELKIENENSSMFNTVEGIFEKPNPMRATVAKRMSESYFSAPVFTFNIEVDATELKV
                     LRAKLIDTVKESTGVKLTMTDLIVMAVSKILPNHQALNSAWTDEGIFRYKDVNIAIAV
                     GLDEGLYVPVVKNANKKSLKEIAKESKELAEKVKTGKLMPADQEGNTFTISNVGMYGI
                     TTFTPIINMPSSAILGVGATQDKFVPVNGEAKIKPIMNLSLTSDHRVIDGTVAAKFLK
                     DLKELLENPLSMLV"
     gene            20977..22707
                     /gene="lpdA"
                     /locus_tag="PC700_001347"
     CDS             20977..22707
                     /gene="lpdA"
                     /locus_tag="PC700_001347"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDF2580662.1"
                     /translation="MSVEVIMPKAGVAMEEGTIVSWLKQEGEEVKIGEPILEITTDKV
                     NMEIESEGEGTLAVIIHKEEGEVLPVFTVIGVIAEKGENQEEVKAKYLSGNVSKEDIV
                     EENQNIEVKEEKINKKECNHDYDVVVIGGGPGGYLSALKAALLGGRVALVEENILGGT
                     CLNRGCIPTKTYIKTAEILEEIDQLSKRGVKVTVDKEQDIKKAIKYKNRVVKKLTAGV
                     GGLLKSRDVDVFNLKASVKEEHKVILSDGKVLDTENIIIATGSKVRILPIKGIESNLI
                     ITSTEALDLETVPEELVIIGGGVIGCEFAEIFNSRGSKVTIVEMEDRVIPRMDKELSE
                     SLKYSLSKKGINVLTKKKVSEFKEEGNNILVCIEGEVPIKADLCLYAIGREANLSGIE
                     DLDIKIDKGSIVVNSKMETSIPSIYAVGDVTGGVMLAHAAFKMGEVAASNALGVNKEV
                     DLGALPSCVYTIPEVASVGITEEDARKKYNVKVGKFNFAGNGRALASGQEQGYVKVVA
                     DAKYGEILGIHMFGCGVAELINHAASFKALEIPTDEASELIFGHPCTSEALMEALADV
                     NGECLHLPKK"
BASE COUNT         9288 a         2389 c         4328 g         6903 t
ORIGIN      
        1 atttaaaata taaagtattt aaagttctga aatctaatca tgaagcaaaa aattagcatt
       61 tggttatcaa taaatataat aaataaaaac tcataattaa tgaaagaata aattcatatc
      121 taaatttcta taaaaaattt aaatttatac aatttagggc tttaaaaatt taaataatag
      181 gtgtaaaata atagtcaaag aaagtcaaaa ataaaaaggt gtgtggtgat attaatggca
      241 actatgacag atataattga aaaatttatt aaagatttga tggaagaaga taatagcata
      301 caaattcaaa gaaatgaact agcaaacttg tttagttgtg caccatcaca aataaattat
      361 gtcttaacaa caagatttac aatagataga ggatattaca ttgaaagtaa aaaaggtggc
      421 ggaggatatg ttcaaataga aaaaatacga aaaagtaaag atggacatat tagagaacta
      481 ctaaatgaaa aaataggaag tcagatatcc tataaaaaag caaaggaatt attagaaggt
      541 ttaaaagagt cggatttgat taacgaaaga gaattaaaat taatattata tgcaatagat
      601 gataaatcat tgtgtatgcc aatatatgaa ttaaaagaga aggtaaggtc taatatttta
      661 aagaatataa taataggatt atttagtata gaggagtgat aatttatgct atgtcaaaaa
      721 tgcaataaga ataaagcatc tgtatattat aataagatag tcaatggaga aaaaacagag
      781 atgtatttat gtagtgagtg tgcaaaagag aatacagaaa tgaattttaa cttagatatg
      841 cctttttcaa tgatggatat attttctaat ttaggttttc aacctaaaaa agaacttgag
      901 gaaaagctag tttgtcctaa atgcaataca acatatagtg agtttaagaa taatgggaga
      961 tttggatgta gtgaatgtta taatgcattt agtagtcaag taaatccaat gcttcaaaat
     1021 atacatgggc atatagaaca tacaggaaaa gcacctaaaa aatcttttta taagataagt
     1081 gtagaaaatg agattaaaga actaaaagaa gatttagata gagctataaa aaatgaagag
     1141 tatgaattag cagctcaatt tagagataaa attaaatatt taaaaggttc tattgactaa
     1201 gattggagtg atattatgaa agagaacata gtcatgaaat caagagttag gctagctaga
     1261 aatttaaata actatccatt tccaaataaa ttagataaag aatgtgccat ggaaataata
     1321 gaaaaagtaa aaaatgcgtt tattaattct aacttagaac aaaaagaaga atttgatttc
     1381 tataaaattg aagatttaga ccaatctaaa aaaatgctta tggttgaaga acatataata
     1441 agcccagatt tagcagaaaa tgataaaagt gcagttatag taaaaaaaga taaaactata
     1501 agcataatga taaatgaaga agaccatata aggatacaaa ctatatgtga tgatttaaac
     1561 ttagaatatg catatagtgt agcaaatgaa atagatgatt tgttagaatc aagtttagaa
     1621 tatgccttta acacaaagct aggttatcta acatcttgtc caactaatac aggaacagga
     1681 atgagagctt ctgtaatgat gcatttacca gcattaagtc aactaggcta tatggatgaa
     1741 ctttataaaa tttcatcaca aataggtata gcaattagag ggatatatgg agaacgaaca
     1801 gaagcattgg gaaatatata tcaaatatct aatcaactta cacttggaag aacagagtct
     1861 aatattatag aaaatgtaag tggacttaca aaagatgcaa tttcaaaaga aataaaagct
     1921 agagaaatat tgcaaaaaaa attaggtata aaactagaag ataaaatatt tagatcgata
     1981 gggacacttg aaaattcaag agtaatgagt agcgcagagg caatgagcca tctatcaaat
     2041 ataaaaatgg gaatagaaat gaactatata gataaactag atttaagggc tatagaacaa
     2101 cttatgatag gtatacaacc agcgcatcag tcaataatgt ataagagtga tgatgttgag
     2161 aatagagata taaatagagc aacatatatt agagaaactt tggaaaaatt aagaggaggg
     2221 atgaattgtg aacttcaata gatttacaca aagagctaaa aaggcaattg atttagcgtt
     2281 tgaatctgct aaaagtctag gacacaatat tgttggaagt gaacatatac ttttaggtct
     2341 tttaagagaa gaagaaggta tagctgctaa agttctaagt aaagttggat ttacagaagc
     2401 ttatttagaa ggcaaaatag ttgatatgga aggtaaggga gaagaaatct cagaagatat
     2461 agtattaagt ccaaggagta aacaaatact tgaattatca ggaatgtttg caaataaatt
     2521 aaaaacaaat tatattggga ctgagcatat attattagca attattcaag aaggtgaagg
     2581 aatagctaat aagattttaa attatgctgg agtaaatgat aggactttag cccaattaac
     2641 aattgatatg atgggtatta gtgataaaaa tcaatacaaa gcagagaata gttacacggg
     2701 caaccagaat caagcagaat ctaaagtact tgataagtat ggaagaaatc ttacactata
     2761 tgcaaaacag aataaaatag accctgtaat aggtagagaa aaagaaatac aaagagtaat
     2821 acaaatatta agtagaagaa ctaaaaacaa tccagtgttg ataggagacc caggtgtagg
     2881 gaaaacagcc atagctgaag gtctggctac aaatatagcc ttaggaaatg ttccagaaac
     2941 actaaaaaac aaaactttat actctttaga gatgggttca ttattggctg gggctaagta
     3001 tagaggagaa tttgaagaaa gaattaaaga agttgtagat gaagttgtta aaaatggaaa
     3061 cataatctta tttatagatg agatgcacac aataataggg gctgggtcta ctggagaagg
     3121 ctctatagat gcttcaaata tattaaagcc agccttggca agaggagaaa tacaagttat
     3181 aggtgcaact acaattgatg aatatagaaa gcatgttgaa aaagattctg ctctagaaag
     3241 aagattccaa ccagttatgg tagatgaacc aactaaagag gactcaataa aaatattaga
     3301 aggattaaga gataaatatg aagctcatca taaagttaaa ataactgatg atgctataaa
     3361 ggcagctgta gaattatcaa ctagatatat atcagacaga tatctgccag ataaagctat
     3421 agacttgatt gatgaagctg catcaaaagt aagattaaaa gaaaatacac ctccttcaga
     3481 aataaaaaaa ttagaactag aaatagaaaa tatagataaa gaaaaagaag aagcagtaag
     3541 atgtcaagac tttgagaaag cagccaaaat aagagatgaa caaggtctac ttaaaaaaca
     3601 attagaagat gttagagaaa gatggaacaa gtcatctaaa cattcagatt tagttgatgg
     3661 agaagttatt gctgaagtag tgggcttatg gacaggaata ccagttaata aaattcttga
     3721 agaagaggct gatagacttt tgagacttga ggaaatactg cacaatagag ttataggtca
     3781 agaacaagca gtaaaatcta tttcaaaggc aatcagaagg tcaagagcag gtcttaaaga
     3841 ccctaataga ccaattggtt catttttatt tttaggtcca acaggagtag gtaaaacaga
     3901 attatctaag gcattagcag aagtgcaatt tggagatgaa aatcaaataa ttagaataga
     3961 tatgtctgaa tatatggaaa aacatgctgt gtcaagaatg ataggttcac ctccaggtta
     4021 tgtaggtcat gatgaaggtg gtcaattaac tgaaaaagta agaagaaatc catattcagt
     4081 tatcttattt gatgagatag aaaaagctca tccagatgta tttaatattc tgttacaaat
     4141 tctagatgat ggtagactaa ctgattcaaa aggaagaact gtagatttta agaatactat
     4201 tgtaataatg acatcaaatg ttggagcatc tacaattggt agacaaaaaa ctttaggatt
     4261 tagtatagct aaaggagacg aagaagaaaa atctcaatat gaaaaaatga aagaaaatat
     4321 aatgggtgaa ttaaaacaaa gattcagacc agagttttta aacagaatag atgatataat
     4381 tgttttccac tcactaaatg aaaatcatat atcaaaaata gtacttttaa tggcagcgaa
     4441 actacaagag agattaaaag aaatggatat aaaattagaa atgagtgatg aggctgttaa
     4501 attaatttct aaatctggat ttgacttaga atatggagca agacctctta aaagagcttt
     4561 acaaaaagag ttggaagatg agctatctga agcaatctta aaaggtaatg tgaaaaaggg
     4621 aagtaatgta gtagcaaaag tcaaagatga aaaaatagtt tttgaaacta aataatttaa
     4681 tagagttgca ttttaaaata ttgcaaatat aagttttata atctaaacaa aattttaagg
     4741 gcaatgaatc tataataatt cattgtcctt aattatttta tttaatcttt acatattgtc
     4801 tttcagatat gctaaaatta tagtatagaa taaaaaatta aggatgataa aagtatggca
     4861 aaaataaaaa caaaatatgt atgtcaatct tgtggatatg aaacagctaa atggcttggt
     4921 aaatgtccag aatgtacaaa atggaataca tttgtagaag aaatagacca aaaaagcacc
     4981 aaaaaagagg tctttataat agataaatct tcatctaaac cagtcagtat aaactcaata
     5041 gaaagtaaag aagaagagag gtttacaaca gatataaatg aattagatag agtgcttgga
     5101 ggaggtatag ttaaaggctc tttagtgctt gttggtggtg accctggtat aggtaaatca
     5161 acacttttaa tccaagtatc tagtaatgtt gccaatttag gaaaaactgt tttatatata
     5221 actggagaag aatcagaatc tcaaataaaa atgagagcta aaagattagg tattaactca
     5281 gaaaatttat atatatttgc tgaaaataat ttaagtataa ttgaatctta tttagaaagt
     5341 gtaaatccag aattaataat tctagactca attcaaactg tcttcagtcc agaaatatcc
     5401 tcggcaccag gaactgtcag ccaaattaaa gaaggtactt caaaattcat gaaaatttct
     5461 aaaaaaatgg gaatttcaac gttcatagtt gggcatgtta caaaagaagg ttcactagca
     5521 ggaccaaaat tattagagca tatggttgat actgttctat attttgaagg agaaagatat
     5581 aatacttata gattagtaag agctgttaaa aatcgatttg gttcaactaa tgaacttgga
     5641 gtatttgaaa tgagggattt aggattagta gaacttgata atccatctaa aattttaatc
     5701 tctgaaaaac caaaagatgt agcaggttca gtaataatat ctactgtaga aggtacgaga
     5761 ccaatgttac ttgaattgca agcattagtt tctcctacaa gttttggtat accaaaaaga
     5821 acatctacag gagtcgatta caatagagta ggtatgttat tagcagtatt agaaaaacgt
     5881 gttggtttgc aaatacaaaa tcaagatgtc tatataaaca tagttggagg aataaagata
     5941 aatgaaccct caattgattt agggatagcc atttctgtag catctagttt tagaaatata
     6001 ccgatagatg aaaatatagc tgttacagga gaagtgggac ttacaggaga agttagagca
     6061 gtaagcttta tagaaaagag aatagctgag tgtaaaaaac ttggatttaa aaagatagta
     6121 gttcctagaa gtaattatga tgttgtaaaa gagaccaaag gcatagaaat atggccagta
     6181 gataacttaa gacaagctat aaatatagta cttgggagga atcaataaat atggagaatt
     6241 ttctagataa taaaaatatg ctatatgcat taaaaatgat atctcctgga actccactta
     6301 gattaggtct aaacaatgta ctaagagcta agactggtgg attaattgta attgcaacaa
     6361 acgaagatgt aatgaaaata gtagatggag gatttgctat aaatgcagaa tattcaccat
     6421 catatctata tgaattagct aaaatggatg gagctatagt tttaagtggt gatgtaaaga
     6481 aaatattatt tgctaatgca caacttatac ctgactattt tatagaaaca tcagagacag
     6541 gaacaagaca tagaacagca gaaagagtag caaaacaaac tggtgctata gtcataggaa
     6601 tttcacaaag aagaaatgtt ataacagttt atagaggaaa tgagaagtat gtagtcgaag
     6661 atatatctaa gatatttact aaggcaaatc aggctataca aactctggaa aaatataaga
     6721 cagtattgga ccaagctgta acaaatttaa atgccttaga gtttaatgat ttggtaacta
     6781 tttatgatgt tgcattagtc atgcaaaaga tggaaatggt aatgagagtt acaagtataa
     6841 ttgaaaaata tgtgatagaa ttgggtgatg aaggaacttt agtaagtatg caattagaag
     6901 aattaatggg tacaaccaga atagaccaga aattaatatt caaagattat aataaagaaa
     6961 acacagaaat aaaagaactt atgaaaaagg tcaaaaattt aaattcagaa gaactaatag
     7021 aattggtcaa tatggcaaaa ctattagggt atagtggttt ttcagaaagt atggatatgc
     7081 ctataaaaac aagaggttat aggattctta gcaaaataca tagactacca acagcaataa
     7141 tagaaaactt agtaaattat tttgaaaact ttcaacaaat tttagatgca tctattgaag
     7201 aattagatga ggttgaagga ataggtgaaa taagagcaac atatataaaa aatggactca
     7261 taaaaatgaa acaattagtc ttattagata gacacatatg atgaatttta aatgaatttt
     7321 actacttttt tcttgaatat aatatacact ttggtctata atagataagt ataactaggg
     7381 ggtgaagcat tgataaggaa agtaacaaga atattgttta ttttacttgg atttacaata
     7441 ggtataacaa cctatttaac attaatgaaa gattttgaaa tattaacatt tggaaaagaa
     7501 acatatggtt atatcgcagc tgtagtagca ggtataataa tagccatttt aggatatctt
     7561 atagaaccat gggttgtaaa taaagtaaaa gaaattgcta aaatagtgga taaagaattg
     7621 tcaaaatatc ctcagactga tatattgtta gggtctatgg gtcttatagt aggatttgtt
     7681 attgcgtatt tattaagtgg attagtaaat agaataccaa tagtaggtgg aatactatcc
     7741 ttactattat atttattcct aggatatctt ggaatgaaag ttgcgcttaa aagtaaaaat
     7801 gatttattta atattggaaa actaggaaga ttagcaaatc ctattaagga taaagataag
     7861 gataaagaaa ataaaaaaga agtaaaagcc attcctccaa aagtattaga tacaagtgta
     7921 ataattgatg gaagaatagc tgatatttgt aagactgggt ttatagaagg aaaacttatt
     7981 attcctgctt ttgttttgga ggaactaaga catattgcag attcttcaga tgatttaaaa
     8041 agagtaagag gtagaagagg tctggatata ttaaatataa ttcagaaaga attaaatata
     8101 gaagttgaaa taagtgagag agactttgat gatatagcag aagttgatag taaattatta
     8161 aagcttgctc aggttttaaa tggaaaagtt gttacaaatg attataactt aaataaagtt
     8221 gctcaattcc aaggtgttga agtattaaat ataaatgagt tagctaatgc tataaaacca
     8281 gttgctatac caggtgaaga tatggttgtt caagtggtta aagaaggaaa agaggctcaa
     8341 caaggtgttg catatctaga tgatggaact atgatagttg ttgatggagg aagaaaatat
     8401 atgaatgaga ctataaaagt actcgttact tctgttcttc aaactcctgc aggtagaatg
     8461 atatttgcaa aacctaaaaa ttaatatgtt caaagaaacc tataatttgg gctgtttatt
     8521 ttaaatagat ggtccaaatt tatttattta taacgataat gtttaatgta ttcttaaaga
     8581 tggggattta aatcagaaag cagggagaat ttatgagaaa aaagattata tctttcttag
     8641 caacatttat cataatatta acatcagcaa gtcagtatag ttttgcagat gatatgtcaa
     8701 cacgtggaaa agtaatattt atagatatga atagaactag tatgagtaat atgttaagaa
     8761 taaaatcatt aagagaagaa ctagacaata gagggtatat tggcttgatg aatataagag
     8821 gcgataaagg aagtgatgat agaaggtcat atgctagtat gggtgctggt ggtagagcta
     8881 atgtggctaa tgaagaagat attaattttg aaagctcgag taaggataga aatatagttt
     8941 ttgaatcagc tacaggaaaa agtgcaaaag gtataaataa tttaactata aataagtcta
     9001 taaatgaaaa tttaaacttt ggagagtatg gttctgtatt agggtcatta ggacaaagtt
     9061 tatctgagaa tggattaaaa gcatctgtat tgggtaattc agatataatt gaaaatggtc
     9121 aacttataaa aaatagaaac ttatgtctaa ctgcaatgga tgaatatgga agaataccta
     9181 atggaaatgt agatactata aataaaaaag acttatctat gccttatgga atatcaactg
     9241 attatgataa attgattgta gagaccaaag aagcttacaa aaataatgat gtggtttttg
     9301 tggagcttgg agacacatat agattagatt tatataaacc aaatttaaat gaaaaaacat
     9361 atgagagtgt gaaagataac atagaaaaaa atatagatgt atatttaaaa aatatatttt
     9421 ctatggttga agaaaatgac actgtctaca tagcaagtgc atttccaagt gatttagatt
     9481 ataaaaataa aagaagactt tctccaataa taaaattaaa tggagaaggg aaaggtatat
     9541 tatcatcttc aactactaga agagaaggta ttgtaacaaa tttagatgtt ggggttgaaa
     9601 tattagacaa ctttaatata aaaaatcaaa atatggttgg aagaaaatat gaacttatta
     9661 acagagatga taataaagaa tttttgatgg atgaatatca aaagatagta tctatttcaa
     9721 gcataaggtc tacaatactg aatggttttg taagtatagt tttcttgtca tggatagttg
     9781 ctatgattgc aattttattt agaaagcata tatcaaaaaa ttataaagaa acaacatttt
     9841 ttatattaaa ggaactttta aaaataggaa tagtaatgcc actgtcattt atgataacac
     9901 caataatgaa tttcaaaaca ccattagcaa tatctttagg aataattata ataacactta
     9961 ctatatatct tataagtaaa gttttaataa agaatgattt aaaaaacatg ctgtttttta
    10021 ctggattaac catagtaatt atggttattg atgctgggtt tggctcatat ttaatgaaaa
    10081 gcaatgttat gagttatgac tgtataattg gagctagata ttatggagtg ggaaatgagt
    10141 atcaaggtgt ggcaatcggt agtgcaatat ttacatttgc aattttactt acatataaaa
    10201 atataccaaa gtggtctata atagtttttt ctttggttat cttaattaca agtgcatctc
    10261 caattatggg agcaaatgtt ggaagtgcta tttctgaatg tgttgcattt ttactattta
    10321 tactattgat atataatgtg aagatagact tcaaaaaaat tgtattatta ggtatagcag
    10381 tattatttgt attaggagtt tttgtagcaa tagatatgat tttgggaagt aattcacatt
    10441 taggtatgtt tgtaaaagaa atttacttta atggaccagg agaaataata caaacattta
    10501 gtagaaagat agagatgaat ttaaagctag ctcaaaccag tgcatgggta aatatactgt
    10561 taactggaat aggtgtaatt ttagttctta tgattaatca gattagatac tttaagcaac
    10621 tgatggatga gtatccaatt gtatttaaag gatttatagc atcaatagca ggatgtttag
    10681 taactctttt agtgaatgac tctgggattg tgtcatcagc tacagccttt atttatgtaa
    10741 ttgtacctat gataacatta agtataaatc tcacagcatt aaaagaataa gtactgtatg
    10801 aataatatgt aaatatgaac acattaattt aacttcttta tttacaaaat aaagaagaaa
    10861 gtttataaaa aataaattat aataaaaaag agtttacctc aaaattgatt attctaatca
    10921 aattttaagg taaacttttt ttattataaa gataaataac atataacctc atattttagg
    10981 tagatttttt tcttatatgt ttaggagtgc atttaaattt ttccttaaaa attctgtaaa
    11041 ataattttgt attagtaaat ccatgagctt ctaatatact gcataaggta tcatttgtgt
    11101 taattaagtc tttataaata tgagaaatac gcacatcatt taaatactct aagtaagttt
    11161 gtcccatata ctgtttaaat tttctacaaa aatattctgg ctgtaagtgt gctaccttag
    11221 cgattttgtt aatagaaata ggcattttgt aattaatctt tgtatattct aaaactggtt
    11281 caaattttga caaatcaagt actgtttttt tcatattttt attacctaca gatattttaa
    11341 agtttgtata taattcaaat aaaagttcaa ataataagct tgtaaagagc agattagctg
    11401 caggaggaga gctaagttta atttctctca tgttttctaa tataagttta actttacctg
    11461 aatttttttt atagttattg tcattggcat taaccttaaa gtcaaaatag caatttttaa
    11521 aatcagggat atatttgttt agaaaagaca atggtacttg caataatata gaggtatctt
    11581 gataagtaca tctagttgag tgaataattc cagaattaat gaacatacac tcattagctt
    11641 ttaaattata agtgtaaccc tccatgttaa tttgtaaatc accagaagtt atataaatta
    11701 tttctaatga gttatgccaa tgatttgcta cataactacc actatcatca aatagtttga
    11761 aattaacatc taaattatca ataggttcaa ttatttcatg tgaaaaatta tttctgttca
    11821 taataatatc ctccttataa ttttaaatta taaatagtaa atttataatg ttcaatatta
    11881 aaaagataat atagactaat aaattagata ataaacatac ttaagaaaac ggataaaatc
    11941 aaatataata actgtatatt aaaataactt aaaaaaatta ttttattata agtgcaaaat
    12001 atacctattt aaataagata agaggggagg attttatgtg tatataaaag gtgtaaattt
    12061 aggaggttgg ttagttttag aaaaatggat gacctcaagt ttatttgaag gcacagaagc
    12121 tgaagatgaa tactatttac caagacagct ttcaagagag gcatatgaat ctagaattaa
    12181 gactcatcgt agtgaataca tcacagagag agattttgca acgattaaat ctatgggatt
    12241 taattcagtt cgtataccag taccatactt tatttttgga gattgtgaac cattcatagg
    12301 atgtgttaaa gaacttgata aagcatttgc ttgggcagat aagtatgggc ttagtatatt
    12361 gatagactta catacagttc caggaagcca aaatggattt gataatggag gaatatcagg
    12421 tatatgtagc tggtcacaaa atccagaata tgtagcgttt acattaaatg tattagaaag
    12481 acttgcaaaa cgttatggga tgagacatga attgtatgga atccaaattt taaatgagcc
    12541 tataacagaa agaatgtgga acataatgaa tgttccaaat cggtttaagg ctgtagataa
    12601 ggagatggca agggggagta aacctaattc attagagttt ttacgtgatt tttatataaa
    12661 agcatataag gtaatgcgtc cttatatgag agaggaaaac gttatagtgt ttcatgatgc
    12721 atttgaatta aaagcatgga aagactttat gagagaggaa gaatttaaaa atattgttct
    12781 agatacacac caatatttga tgttagctga atcagatgga tgtgaacaaa gtattgatag
    12841 ctatctcaaa tatattagag aaaattatgc taaagatatt ttacaaatgc aaaaatattt
    12901 tcctgttata tgtggggagt ggagcttatt taattcatat gcttgtggta tagatacaaa
    12961 tggaggtcaa tcacctctaa atgggattga atcaaatata gataaattat caaaagacga
    13021 taaaagagaa ttatatagga agatagcaaa agcacagctt gatgcatgga gaaatggaag
    13081 tggtcactac tattggaatt ataagttatt actagataca gttaatgaag aaggttggat
    13141 aggatgggat agttgggacc taggcaaatg tgtagctcaa gaatggtatc ctatagaata
    13201 ttaaactaaa attggaggaa aagatatgat taaaaaccct atacttccag gttttaatcc
    13261 agatccttgt atttgtagaa agggagatga ttattatctt gtagtttcat cttttgaatg
    13321 gtttccaggt atacctgttt atcactctaa agacttaaaa aattgggagt tatatactca
    13381 tattttgaca gatgaaacaa aaatagattt gaagaagctt ccttcatcta aaggaatttg
    13441 ggcaccttgt ttgacttatt gtgaagaaga agatttattt tacatagttt atggaattat
    13501 gaactctatg aatgcaagat attttgatgt tgataattat ttaattacat caaaagatat
    13561 aaagggagag tggagtgaac cagtatatat acactcatca ggttttgatg cgtctattct
    13621 tcatgatgat gatggtaaaa agtggattgc atcattagac tgggagacta gagaaggata
    13681 tgaaaaacct ggtgttatat gtttagtgga atactgtaca aaaaagaaag aaatagtagg
    13741 atatccaaag agaatatggt cagggggaac tgatagaggt tgtattgaag caccacatat
    13801 aacaaagcgt ggagattact attatataat gtgtgcagaa ggtggaactg gatatggtca
    13861 tagtgtaact atgggaaggg ctaaaaatat atggagacct tatgaaaaag attcaatgaa
    13921 tccaattgta acttcaattc caggcgattt ttatgaaaga catgaccctg accatctaaa
    13981 gcctaaatac tataatccag aatcaaaact tcaaaaatct ggtcatggaa gctatataga
    14041 aacaacatca ggtgaagtat atttagttca tctaacatct agaccatttg taccagagtt
    14101 aaggtgtact ttaggtagag aaacagctat tcaaaaaatg aaatggacaa aggataattg
    14161 gcttcgaatg gaagatgaaa gtaatttggc aaaagaatac gtatcagaaa gtaaactaga
    14221 agaacattta gtttcatcta taccaagttt tgatgacttt gattcaaatg tattaggttt
    14281 acagtactat gctcctagga tttcaccatt atcatttgca gatgttaagt ctagaccagg
    14341 atatgtaagg attagaggtc aagaatcaag aacatcatta aataaagtaa gcatattggc
    14401 aagaaaatta acttcagtat atgcaagaat aaccactaag atggagtttt acccagaggt
    14461 acatcagcat agtgcgggtt tgataatgta ttatgataat atgaattata taaatttaag
    14521 aaagtattat agtgaaacat tgggtcaaag tgcactatca ataatacatc ttgaaaatgg
    14581 agagaagacg gaatttttaa atactaggat accaataaaa gatataccaa tatatcttcg
    14641 tctgtatata caaggtcgta agtcatattt tgaatggagt tatgatgaaa aaaactacca
    14701 aagaatagga aaggtatttg atacaactaa gttttcagat gagtactgta aatatggaga
    14761 atttacaggt acgtttatag gattaacttg tgcagatcgt gtaaagcata aacactatgc
    14821 tgattttgat ttttttgaat atatagcaga tgaatcaaaa gatgtcgatt aataaaatca
    14881 ttactaaaat tttcagagca aaggtaaatc ttatgctctg aaataagaaa cattagaagg
    14941 caaatcttac aaaaaggagt atggtattat gaatacagaa gttaatgtta aagcaactac
    15001 gattagatat gaaaagattt cattttttga aagaatttct tatggttgtg gggatttggg
    15061 gtgcaatatc atatactcag ctatgtcagc atttttgctg ttttattata ctaattatgc
    15121 agatgttagt gcagcagctg taggtagtat tatgcttgta tctaggatac tggatgggtt
    15181 tagtgactta acaatgggaa ttatagtgga tagaactaaa tccaaatacg gtaaggctag
    15241 gccttggatt ttgagaatgg cgattccttt tgctatagca gcagtattat tattttctgt
    15301 tccatcaaat cttggtatca cttctaaatt aatttatatt tttattacat ataacttagt
    15361 ttctactgtt atttatacag caatcaatgt accctatgca actctaaatt cacttataac
    15421 tcaaaaccag tatgaaagag gagtattaag tatatttaga atgatattag ccacttgtgg
    15481 taccttacta ataaatggac ttacactacc tctagttgaa tactttggaa ataatttatc
    15541 agcatggact aaaacattct ttgtatttgg aatagcatct ataatggtat tttttataac
    15601 atttactgga accaaggaaa gagtaaaagc agttaagcaa gataggaatg aagtaatacc
    15661 atttaaaata ggtattaaat ctttatttag aaataagtac tggatacaaa taacattatg
    15721 tttagtttgc atatttattg tatttgcgat aaatggtggt tcatccgtat attatgcaaa
    15781 gtttattttg ggagatgaaa aattatttgg tcctataaat atggtttcaa atatatccca
    15841 aattatagct atgtttatgg ttgctccttt tataaaaaaa tttggaaaaa gaaatgtatt
    15901 aatagtaggt tcaataatat taatatcgtc aaatgttatg tttataatag ttggacaaaa
    15961 ctatatagga gtaatatttg caagtgctat aaagggaata ggaagtgctg gaatagcatc
    16021 aacaatgttt gctattgttt cagatactat tgaatatgga gaatggaaga ctggatatag
    16081 aactgagggg cttataaata gtgcatctag ttttgggttt aaagtgggaa atggattagg
    16141 ttcagcaata ttaggtgcag tattatcaat tggtggttat gtaggaacat cagctactca
    16201 aagtgattta gctatagtat caataaaagt atgttttata tacctaccta tatttataac
    16261 aatattacaa attatcataa tgttttttta taagcttgat aaagaatata gtactatact
    16321 taatgaatta aatactaaat aatgaaaaag tttcttataa agtcttaagt ttaattaagg
    16381 tctattgtaa atgttcccta taaaaactat tatatattta tgattattga agaattttta
    16441 gagaagcgaa taataaaaat ataagaagac tttaaatgat gaaatggttg gtcttttaga
    16501 aatgatttaa taagttattt ttatttaaag cattataagt ggacaattaa tttttaattt
    16561 atggagatga tatttaacaa ttagaagtaa tatataatat gcgctaagaa gcaatttttc
    16621 aactatttat gaaggtggtg gtacaagaaa agtatcacct tttttttgtt atacaaaaaa
    16681 acagttgact ttttatatga accccttcta taatgttact gaaattaagt aaaatgattg
    16741 aacaaatgta aagataattg aaaagcttga ttttggagga aatcaaatga aaaaaattgg
    16801 agacttaaga ttaatgatga agtgttgttc actttattac gaagataatt tgaatcaaca
    16861 ggaaatagca aatcaattgg gaatatcaag accaacaata tcaagaatat taaaagaagc
    16921 ttttgaacaa ggaatagtaa aaatacagat agttgatgtt ttgaaaaatg attaccaaaa
    16981 aatagagaga agtttggaga gaaaatataa attaaaagaa gtaatagtag tagatgataa
    17041 acaagatgca ctaacacaaa aacaagaact agcaagagct gtatctgaat atttaactag
    17101 agtagtaaag gaaaatgata taataggtgt ttcaatagga acaacactaa aagaaatacc
    17161 aagatatgtg gaaaaaagta actgtaaaaa tgttacattt atacctctat taggagggat
    17221 tggagacaat gaaatagata ttcatgcaaa tcaaatagca gtaagtctag cgagggcttt
    17281 tggaggtgat tttaaattat tacatgctcc agctgttatg tctgacttgt ctacaaaaga
    17341 gaaactttgc aaagacgaaa aaattaaaga agtactagat ttaatagata aaacaactat
    17401 agcaattgtg ggtataggaa atcctatgag tttaaactca acaataatgg cttctggata
    17461 catgaatgaa gcagatatag aagatttaaa aaaatacaac tcaataggtg caatttgttt
    17521 acaagccttt gataaagaag gaaaaacttc tatacttgaa tttaatcaaa gagtattagg
    17581 agtgaaactg gaagatttaa aaaagataaa aagaacaata ggtgtcgctt caggagatga
    17641 aaaaatagag gcaataaagg catcattaaa agcgaaattt ataaatagtt tagcaattaa
    17701 tcacagttta gcacttaaat tattagaaga ttgtgactaa tagaaagata gataaaagtt
    17761 aacaggaggg aatgtatatg tcaatatcaa aagaaacatt gcttgaaatg tataagagaa
    17821 tgaatcaggc tagaaaattt gaagagaaag ttagttggtt ttttgcaaga ggtatggttc
    17881 atggaacaac tcacttatct gtaggtcaag aagcgtcaag tgtggcggct gtaatggctt
    17941 tagaaaaagg agatttagta tcattaactc atagaggaca tagtcaattt ataggaatgg
    18001 gaatagacct aaataaaatg atggctgaac ttatgggtaa agaaactgga ttttgtaagg
    18061 gaaaaggagg ctcaatgcat atagcagata ttgagtcagg taacttagga gcaaatggag
    18121 ttgtaggtgg aggtcttaca atagctccag gagctgcact tacacaacag tataaaaaaa
    18181 caggcaaaat agttttatgt agttttggtg atggagcatc aaatgaaggt acttttcatg
    18241 aaggaataaa tttatcttca atatggaaat tgccaataat attttactgt gagaataatt
    18301 tatacggtat gtcaacaagc ataaaacgtc atatgaatat agaaagtatt gccactagag
    18361 cagcttctta tggaatagaa ggaatatcaa tagatggata taacccaata gaagtatacg
    18421 aaacagtaca aaaagctgct gagaagtgta gaagaggaga aggacctgta ttaattgaaa
    18481 gtagaactta tagatggtta ggtcattcaa aatcagatgc aaatgtatat agaacaaaag
    18541 aagaaataga atcatggaaa gcaaaagacc ctattgaatt tttaaaaaat tatcttatag
    18601 aaaataatct atctaatgaa gatgaattag ataagattca agaatttgca aagcaatcaa
    18661 tagaagatgc agtagagttt gctcaaaata gcccaaatcc aaaaatagag tctttattag
    18721 aggatgtata tgcagactaa gaatggtaaa ggaggaaaat aaaaatgagt acaagggaat
    18781 taacatatgc acaagcaata aaggaagcaa tgtcagaaga aatgagaaga gatgaaaatg
    18841 taatatttat gggagaagat ataggaatat atggaggggc atttggtgta tcagttggta
    18901 tgatagatga atttggacca gaaagagtaa gggatactcc tatatcagaa gcagctatag
    18961 caggagcggc agcaggtgca gcagcaactg gtttaagacc tattatggaa gtaatgttta
    19021 tggactttgt aacaatttct atggatgcaa ttgtaaatca agcggcaaaa atgagatata
    19081 tgtttggagg aaaagcacaa gttccaatgg ttgttagatg tccaggtggt tctggaactg
    19141 gttcagcaga gcaacattca caaagtttag aagcttggtt ttgtcatgta ccaggagtaa
    19201 aagtagttgc accttctaca ccagcagatg ctaagggatt gttgaaagca gctataagag
    19261 ataataatcc agtcatattt gttgagaata aattgttata cagaaaaaaa ggttttgtgc
    19321 cagaagatga ttatgtaatt gaaattggaa aggcagatat taaaagagag ggtactgatg
    19381 ttacagtaat tacttatgga agaatgttac aaagtgttga agaagcagca gagactctat
    19441 caaaagaaaa tataaatgtt gaaattatag atttaagaac tttatatcca ttagataaag
    19501 agactatagt aaagagtgta tgtaaaactg ggagagtttt aatttgccat gaagcagcta
    19561 agactggtgg gctaggtgga gaaatatcag ccctgataac agaaagtgaa tcatttgatt
    19621 atttagatgc tccagtaaaa agaatttgtg gaaaagatgt gccaatacca tataatccag
    19681 agttagaaaa agcagttgtc ccaagagttg atgaaataga agaagcaata aaatctttaa
    19741 ttgttagata aggagggata gatatggtgg caaataaaat aaaagccaca ccagcagcta
    19801 gaagtcaggc aagaaaagac aatataaaat tagataggct gattggaagt ggagaaaatg
    19861 gaaggattca tttagtagat gtgttaaatt atttaaaaga taataaagca aatactactc
    19921 ctttagctag aagaatagca gaagatttaa atatagattt agaaactatt gtaggtactg
    19981 gatatagtgg aaagataaga aagtgtgatg tggaaaaatt aactgcaaaa gaaactattg
    20041 tatcaaataa tacatctaaa tatagtgaga aaaaagagct gaaaattgaa aatgaaaatt
    20101 caagcatgtt taacacagta gaaggaatat ttgaaaagcc aaatccaatg agagctactg
    20161 tagcaaaacg aatgtcagaa agttatttta gtgctcctgt atttacattc aatatagaag
    20221 tagatgctac agaattgaag gtacttagag caaagttaat cgacacagta aaagaatcaa
    20281 ctggagtaaa attaactatg acagatttaa tagtaatggc agtatctaaa atacttccaa
    20341 atcatcaagc cttaaattca gcatggacag atgagggtat atttagatat aaagatgtaa
    20401 atatagctat tgcagttggt ttagatgaag gcttatatgt tccggtagta aaaaatgcaa
    20461 acaaaaagtc tttaaaagaa atagcaaaag aaagcaaaga acttgcggaa aaagtaaaga
    20521 cagggaaact tatgccagca gaccaagaag gtaatacatt tacaataagt aatgttggaa
    20581 tgtatggtat aactacattt acacctataa taaatatgcc ttcaagtgca atcttagggg
    20641 taggtgctac acaagataag tttgttccag taaatggaga ggctaaaata aaacctataa
    20701 tgaacttatc attaacatca gaccatagag taatagatgg aacagtagct gcaaaattct
    20761 taaaagattt aaaagaattg ttagaaaatc ctttatcaat gcttgtatag gttttgatat
    20821 ttcgttactg atataaatag tttgaataga ttatattatt ataattgttt cagtaaattt
    20881 aaagctttta tatattaaga attattttta acagtaacta tatgtgaata tgatttgctt
    20941 aattaaaata tatgaaaata aataggagga gagcctatgt cagtagaagt aataatgcca
    21001 aaagcaggag tagccatgga agaaggtact atagtatctt ggctaaagca agaaggcgaa
    21061 gaagtaaaga ttggagaacc tatactagaa ataacaacag ataaagttaa tatggagata
    21121 gagtcagaag gagaaggtac acttgctgta atcatacata aagaggaagg tgaagtatta
    21181 ccagtattta cagtaatagg tgtaatagca gaaaaagggg aaaatcaaga ggaagtaaaa
    21241 gctaaatatt tatctggaaa tgtttcaaaa gaagatatag ttgaagagaa tcaaaatata
    21301 gaagttaaag aagaaaaaat aaacaaaaaa gagtgtaatc atgattatga tgtagtggta
    21361 ataggaggag gacctggggg atatttatca gcattaaaag cagctctttt aggtggaaga
    21421 gttgctttag ttgaagaaaa tatactagga ggtacttgct taaatagagg ttgtatccct
    21481 acaaagactt atataaaaac agcagaaata ttagaagaaa ttgaccaatt atcaaagaga
    21541 ggtgtaaaag taactgtaga taaagaacag gacattaaga aggccataaa atataaaaat
    21601 agagtagtta aaaaacttac agctggtgtt ggcggattat taaaaagtag agatgtagat
    21661 gtgtttaatc ttaaagctag tgttaaagaa gagcataaag taattttatc agatggaaaa
    21721 gttttagata cagaaaatat aataattgct acaggttcca aagtaaggat attacctata
    21781 aaaggaatag aatcaaattt aataataact agtacagaag cattagattt ggaaacagtg
    21841 ccagaagaat tagtaataat tggtggtgga gtgattggtt gtgaatttgc agagatattc
    21901 aattcaagag gttctaaagt aactatagta gaaatggaag atagagtaat cccaagaatg
    21961 gataaagagt taagcgaatc tttaaaatat tctcttagta aaaaaggtat aaatgtatta
    22021 actaaaaaga aagtttctga atttaaagaa gaaggaaata acatattagt gtgcattgaa
    22081 ggtgaagtgc caataaaagc agatttatgt ctatatgcta taggaagaga agctaatctt
    22141 tctggcatag aggacttaga tattaaaata gataaaggtt caatagttgt aaatagtaaa
    22201 atggaaacaa gtataccaag catatatgca gtaggtgatg ttactggagg tgtaatgtta
    22261 gctcatgctg cttttaaaat gggagaggta gcagcttcaa atgctctagg tgtgaataaa
    22321 gaagttgact taggtgcatt acctagttgt gtttatacaa ttccagaagt agcatcagtt
    22381 ggtattacag aagaggatgc tagaaaaaaa tataatgtaa aagttggtaa gtttaatttt
    22441 gctggaaatg gaagagcttt agcttctggt caagaacaag gatatgtgaa agtagtagca
    22501 gatgctaaat atggagaaat tttaggtata catatgtttg gatgtggtgt agcagaactt
    22561 ataaatcatg cagcatcatt taaagcatta gagataccta ctgatgaagc aagtgagttg
    22621 atatttggtc atccatgtac ttcagaagct ttaatggaag ctttggcaga tgtaaatgga
    22681 gaatgtttac acttacctaa aaaataaatc taaacataaa ttattaattt aagtctatta
    22741 ttaatattaa tttatatcat aaaattttaa aacacttact tagccatacc ccttattatg
    22801 caaataattt atttgtataa taaggggtat tttttatgga aaaatataga atatagaatc
    22861 cttgtagttt ttgttgtaca aaaaagatag taatataaaa taacatta
//
LOCUS       DAMJWP010000040        22106 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.041, whole genome shotgun sequence.
ACCESSION   DAMJWP010000040 DAMJWP010000000
VERSION     DAMJWP010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22106
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.041"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            215..1036
                     /locus_tag="PC700_001348"
     CDS             215..1036
                     /locus_tag="PC700_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2580663.1"
                     /translation="MGYKLIVTDMDGTLLGNNHKVTDENKTALQKVIKSGINVTLATG
                     RAFDSAKCNVDFLKEDMPIIACNGSLIREQNGNIIYSNKIDTRTCLNILDVLDKYDIY
                     YQCNSIDSMLSKKIEGREDRLSVFLGSETEVIVKDDLREEIFKKDILKFVIIEEKNPS
                     ILDEIRKELRKVQGIKITSSWPNNIEVMNEGVDKGNAVKILAEKMNIDREDIIAFGDN
                     YNDIEMIKFAGLGVAMGNAEELIKQEADYVTDTNQDSGVAKAIYKFLELKESLSS"
     misc_binding    1115..1326
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1413..2729
                     /gene="argH"
                     /locus_tag="PC700_001349"
     CDS             1413..2729
                     /gene="argH"
                     /locus_tag="PC700_001349"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HDF2580664.1"
                     /translation="MKLWGGRFRKAENQLMEEFNKSFGYDCVLYKKDIEGSVAHVHMQ
                     VKCGLLTEEEGKSITEGLKGILEDVENGKLVLDGEYEDIHSFTEINLIQRIGDVGKKL
                     HTARSRNDQVAVDMRLYAKEKANDLVGLISEFKATIKDVADKNPVMMPGYTHLQRAQV
                     VTFKHHLMAYYSMFDRDEKRIKNAIEILDESPLGCGALAGTTHDIDRSITCEQLGFKK
                     VVDNFMDGVSDRDYLLELMSDFSIIMMHLSRLSEELILWSSQEFGFVEIDDLYTTGSS
                     IMPQKKNPDGAELIRGKTGRVYGNLFGLFTVMKGIPLAYNKDMQEDKEGFFDSVHTLE
                     MCIQIMDRMIATLKVNEDKMKQAVKNGFLNATEVADYLVKNNVAFRDAHGIVGSIVIY
                     CEDNKKAIEDLTLEELHKFSDAFKEDIYDFIDYESILNKGIKKNLK"
     gene            2978..3316
                     /locus_tag="PC700_001350"
     CDS             2978..3316
                     /locus_tag="PC700_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HDF2580665.1"
                     /translation="MNLLVIGGHERMEKDYMVMAKKKGYKTKVYTTMSSKLNNSIGRP
                     DAVVILTSTVSHKMSRTVESQAKKQDILIVRHKNSSKVAFNECLDMVDECLGNCSKCK
                     LKDKQKQKNI"
     gene            complement(3443..4732)
                     /locus_tag="PC700_001351"
     CDS             complement(3443..4732)
                     /locus_tag="PC700_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDF2580666.1"
                     /translation="MKRNLSLLLICLLIFTSFLGSSNISFADNEPAIVAKHAVLMDYE
                     SGKILYNKDGNSKLYPASTTKVWTACLVLKEVKDLNQVIEIKDLPQIDGSSMYLKNGE
                     SFTVKQLLDALLVHSANDAAFVLARYVGGGNVQKFIDLMNSEAKKIGATNTHFNNPHG
                     LPDPNHYTTAHDMALIAREAMNNDTFRQIVKTKSLKFEATKAYPYERYFVNTNKFLTS
                     HDKITYKGQPINIKYDIVDGIKTGYTDAAGKCLLSSAVKDGRRVIVAVFNSTNADLYL
                     DSRILIDYGFDNFKCATIVDKEKYTDTKKVLFTKQHELIYEPKNSYRIFLEKDESQGN
                     YDTKTELNKIDLPIKKGAKVGTLNVYNNGKLENSIDLIAKNNLDSSLPFFTGNNILMT
                     FVKIIAGILVLLVLFIIASNIKKKKRKKARSKKNMKK"
     gene            4812..5516
                     /locus_tag="PC700_001352"
     CDS             4812..5516
                     /locus_tag="PC700_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HDF2580667.1"
                     /translation="MKKKVKFISFFVLFILIISSIIVYKSLIFKDDIYVVSKKNLPNE
                     KTHVVENEIKGEDNNHNKVTKDIDKNFQNNKITIYISGAVNRPGIVTIESDKRLYDAV
                     ELLGGTTKEADLNGVNLSVRLEDEQHYIIPKIGEATSVTSNDDSNKPNQKNESRGEPK
                     NKSNKNISNESKVNINVATIEELDSLPGVGEATANKILQHREENGQFSSIEEIKNVNG
                     IGDKKYENIKDLICVD"
     gene            5608..6654
                     /gene="selD"
                     /locus_tag="PC700_001353"
     CDS             5608..6654
                     /gene="selD"
                     /locus_tag="PC700_001353"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:5656..5658,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HDF2580668.1"
                     /translation="MKKEQKKLTEMTTAGGUAAKIGPEVLASVLSQLPKNDNIENLLV
                     GLDTADDAAVYKLNDDMALIQTLDFFTPMVDNPYVFGQIAASNSLSDVYAMGGKPLVA
                     MNIVCFPSCHDMDVLAEILKGGFDKVKESGALLVGGHTVDDKEPKYGLSVSGIVSPNK
                     VLSNATAKPGDKLIITKPIGVGVLNTAMKEGMVEQHIADKVIEIMIHLNKYAAMSFEK
                     FDVNSVTDITGFGLLGHTLEMAKASEVSIEIESKHVPIIEGAIEMAQMGIIPAGMYKN
                     MHYVSKDVEVVGNIEVAVQDILYDPQTSGGLLISVKEELAEELVKDMKLNGAIEAKII
                     GSVVPKGEKYITVL"
     gene            6672..8084
                     /locus_tag="PC700_001354"
     CDS             6672..8084
                     /locus_tag="PC700_001354"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HDF2580669.1"
                     /translation="MIVLSKRELFAMLPSVDEVLSDNRIVEIINEYPRSLVLESVREV
                     IDLKRQFILRLKEDASNSVTIEFGEIIESAIERVKLNYSLSLKKVINATGTVIHTNLG
                     RSLLSEDIKDELWCAASRYSNLEYDLDNGERGSRYSHLTSTIKRLTGAEDVLVVNNNA
                     AAVLLVLSTMAKGGEAIVSRGELVEVGGSFRIPSIMALSGAELVEVGSTNKTHLKDYK
                     EAITEDTNVLMKVHTSNYRIMGFTESVSIEELVNLGKKYKLPVIEDLGSGVFIDLSKY
                     GLSYEPTVLDSIRQGADVVTFSGDKMLGGPQAGIIVGKKEYIEKMKKNQLTRALRVDK
                     LTICALEATLRMYLDETKAIENIPTLKMLTYKIEELEVKANKLFEKITALNLNANINI
                     EDGFSQVGGGSMPLETISTKVISITPEHMNVSSLEKKLRLSEAHIIARVYDNKYVLDV
                     RTIFDDEFDVIVAELRKAFN"
     gene            8198..10081
                     /gene="selB"
                     /locus_tag="PC700_001355"
     CDS             8198..10081
                     /gene="selB"
                     /locus_tag="PC700_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HDF2580670.1"
                     /translation="MKNVVIGTAGHIDHGKTTLIKALTGRETDTLDEEKKRGISINLG
                     FTYFDLPNNRRAGIVDVPGHEKFIKNMLAGASGIDIVLLVVAADEGVMPQTVEHLDIL
                     SFLNIKNGIVVLTKSDTVDDEFRELVKEDICEKIKGTFLEDAEIIEVDSISKRGIDTL
                     IEKIDKMSDDIEDKNENSPARLNIDRVFSIKGFGTVITGTLIEGKISIEDDLVIYPQV
                     LKTKIRSIQVHGENKDTAYAGQRTAINISNVKVDDIKRGDVLAAPNSLEESMMLDVKL
                     SLVNHLDKGLKHWDRLRLYHGTREILCRAVPLDKELIESGESGYVQLRLEESIVSKKG
                     DTFVVRRYSPMETIGGGVIIDPSPKKHKKFDEKVIEALKIKEKGELKDIIEEYLKRNL
                     KNYPNIKEIMSYSGAHEEDVKRALEALISEDKVFIIGNMYMHINQYNKLKENTIKLLS
                     EYHKKYRLRKGILKEEVRSKIESNFKTREMDILLEKLSTENAIKIENNIVSLLDFEVI
                     LNDKQKEIAKKIEKRLKSCGVSSILTIDEVSEGNHNYAEVLESMIGNKVEKLDDLYIM
                     DKDIYENAKNILINYIKENKEITLGEYRDLIDSSRKNCMIILENFDRNKITKRVENKR
                     ILF"
     gene            10186..12924
                     /locus_tag="PC700_001356"
     CDS             10186..12924
                     /locus_tag="PC700_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation-associated two-component system
                     sensor histidine kinase"
                     /protein_id="HDF2580671.1"
                     /translation="MKNIIPDVKPDIKEREDMSLLFIIMGSVLLLFIIKDIEKYETLL
                     HAIYTIFLVATGVIIFNTTKFKVDSFSTFLGLIFIATGVLECIYLFNSLGIKTKSIME
                     INITISAITDLFPILGVYLSFKFVKDNKQIYSSIILFIATATLTITSLFIISGICDYL
                     GNGKSQYALGVIISIFIIIISIISGIELNSSSNKGKWEYDEKKIFNRIIVIIILSRVP
                     NLLHIVIDNRNVERILSQIIINVALYYLYNYIVSKNIKKTALELHDTNEELTKKTESL
                     KEKNKKLIYETEKIEELKGILMKRESRLQSTLDVAVNSIVVFSKDGEITYANKSFRNT
                     FGDYKEQDKLTDKIKNFNDLIESIHYVFVHEKNAEKLISTSDNKVYQAIFAPLIIASQ
                     TEGVLCVLEDKTKKKEYERKLIEANKRSKDFLESIGDGIVVLEGNNKIYVNNACREIF
                     KNNLESIDFSLVCKSEESIEKRYVIDGEVKYVEMSFSQYTNAGTNKTIIVIRDTTSRK
                     ISQIKLEESQSSYSRFIDILPDGICLLKEDLSINYANKSFLDMLAFTNIDDIKDSNIK
                     LLMNANTEEKMKFTDNMAKVLNENRSILLLEYELINSYDDIVEVELSALPFAIYNTRY
                     IMLIIKDLVHKKYSEQAEKELLERFKTDKIKTEFFANMSHELKTPLNVISSSNQLVDS
                     FYRNEKIKDYNNNIKSHVDLVRQSSYRLQRLINNIIDLTKMESGFYTLKLAKYNIVSV
                     IEDLFMNIEEYALRKDIKILFDTDLEEINVYIDKVEIERIMLNLLSNCIKFTDNGGWI
                     YVSIHYKIDKVIISVKDTGVGIPQDKLELIFEEFSQVDKTLSRNTEGSGIGLAIVKNL
                     VSLHGGDIEVVSEVNKGTEFLISLPMKGFSSEHYTEDKRIYNIQEKIKIEFSDIYY"
     gene            complement(13043..13987)
                     /gene="manA"
                     /locus_tag="PC700_001357"
     CDS             complement(13043..13987)
                     /gene="manA"
                     /locus_tag="PC700_001357"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HDF2580672.1"
                     /translation="MEPLFLKPIFMDRIWGGTALKDKFNYEIDSPTTGECWAISSHKN
                     GDCLIENGKYKGKKLSELWNKNRELFGNTPGDKFPLLTKILDANDNLSVQVHPNDEYA
                     KKNENGELGKTECWYVIDCSDDAEIIIGHNAKSHKEFVNMVNNNEWDKLLRKVNIKKG
                     DFFYVPSGTIHAICKGTLILETQQNSDTTYRVYDYDRTDNSGNKRELHVQKSIDVTNV
                     PHINFDTDYKIVSTSDFKCTTFVSNEFFSVYKLDVFGKCNFTHNTPFSLYSVLDGNGK
                     LIHNSVEYNLKKGMHFILPNDFGDFSFEGNLEIICSHI"
     gene            14160..15521
                     /locus_tag="PC700_001358"
     CDS             14160..15521
                     /locus_tag="PC700_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2580673.1"
                     /translation="MELFKAESKKYEKEFLKLLEQWVSIPSFYDRKTVSKDMPFGKGV
                     YDALNWFENLGRENNFKVKNIDNHAVQIEYGNGKEYVDIFGHCDVVNPGEGWDSEPFK
                     LNIIGDKLVARGVSDNKGPMIVNFLALKMIKDLDINLKRKVRLIAGGNEESGFKCIKH
                     YYSKEPYGVCGFTPDAKFPVLNGEKGGAIIKLISNIDDKSLYISGGIEFNTIPDKVYI
                     KNVEKLGKDNICFDINNISINYDNGNYIVYGKGGHSSKPEKSINPILATIKLLSENID
                     EKWTKDLYKLINQDNINGNLFGLNIEGKCGILSIVPTIINIVDGKLEVVLSVRYPEIL
                     TIEDIIKKFNLYMEQNNINKFELIGENLKQANYIDRNSKLVRSLHDIYIKYSGDLKND
                     VRVTSAGSYASEMNNSVIFGCEFPDGSFGNVHSANEFASLDRFITAIGIYAEAIITLC
                     NKI"
     gene            15609..17498
                     /locus_tag="PC700_001359"
     CDS             15609..17498
                     /locus_tag="PC700_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2580674.1"
                     /translation="MITKKHAIIVKSLSNKDGYMTSNELAIKLDVSTKTIKRYIADLN
                     SVLSKYDLVIASSRGIGYKLSGSKNNIARAVKEANKYIDGFLDDSEESRMSNIICMLI
                     NRNYMSIEAMAEELNLSIAAINKLSSKLKRKLEKYDLVIKSKPYYGSYIHGEEINIRQ
                     LITDYAIKLDEKSKVKVFLDDISENDIYCIESILEKHLREKDTIISDKDFNLLLSKII
                     VSVFRSKRGHSNNINLMDTSYRFHNYYFIENLMKEISDKIGFKLIEDEVIYISNYSGV
                     IAYKGTQGRKNTSEIEERISSVISSALQDIFLISGSDYTKDDEFMVAISDHIKRFLNR
                     ARANVKSNNPLLHQIKEKFPIAFNLAVFLSNKLETEFNLKLDEDELGYIAIHFAASNE
                     RMKKNTSKKICIVCHYGIGTGQLISEKLKQNISDLSVVGVYPVRYLDMAISQDVDLIV
                     STVELKGYEKPVLYIENIFDDSLIENVNKAFYEKEERRKIISNMFDEKAFFSIKASTK
                     EEVIISLSNKLKERDFIEESSIKSIIDRENISSTEIGNLVAIPHTIVKGDKKSIIGVG
                     ILENPIIWDKQEVQLVFMVFFNTKEKHNFSIFKYLYNFIKDEGGVRGIIKICDFNKLM
                     ALIGN"
     gene            17513..17968
                     /locus_tag="PC700_001360"
     CDS             17513..17968
                     /locus_tag="PC700_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2580675.1"
                     /translation="MGTNIFNKEYVFLNVDAKSKVEVLKFISKKAKDLNLAEDESLVY
                     EGLMAREEQFTTNLGESIAIPHTKNDAIENPAVVVLKFNEDVVWNEGEDKVKLAISLL
                     MPGKSKENIHLKLLSSLSRKLINKEFKDSLLKSDNVEEISNLINEALGL"
     gene            18027..18344
                     /locus_tag="PC700_001361"
     CDS             18027..18344
                     /locus_tag="PC700_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDF2580676.1"
                     /translation="MNKKLVALCACPMGLAHTFMAAEAIEQAAKALGYEAKVETQGAD
                     GVQNELTRDDILGATMIIHAVAITPEGMERFDGCEVYEVELQEAIKNAEGVIKEIEED
                     LGI"
     gene            18369..19631
                     /locus_tag="PC700_001362"
     CDS             18369..19631
                     /locus_tag="PC700_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDF2580677.1"
                     /translation="MAIKKKVINSSGASTTGGNNVGKSTPSSPTQSKGNGKWKEISKH
                     IMTGISYMIPVLVMGGLIGALSQLIPYAILGLDPSVGIVDAMNSGEFTGFKLSLLNIA
                     QLMSNFGFTLFGFAIPLFAAFCANSIGGKTALIAGFIGGYIANKPVGVPQFIDGQWTE
                     VVPVASGFLGAILIAFIIGYFVKWLNKSIKVSHNWLAFKTTFLIPLIASLACMVLMIF
                     IITPFGGLINESMKNFLTAAGAAGEYVYATALAAATAFDLGGPINKAAGFVALGLTTE
                     NVLPITARTIAIVVPPIGLGLTTLLDKRLVGRRVYDRQFYQAGKTSIFLSFMGISEGA
                     IPFALERPGFVIPLNIVGSVIGAITGIILGAIQWFPESAIWAWPLVDNLFGYIIGIAV
                     GAIFIAVGNIFYRNKLIKDGKLVVDYID"
     gene            19736..20800
                     /locus_tag="PC700_001363"
     CDS             19736..20800
                     /locus_tag="PC700_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2580678.1"
                     /translation="MDKIEKVREYFREYDIDGFLINSPTNKFYVGNLFSSSGYVFITK
                     ESQYIIVDFRYFEEIKRKSSLFNVVLMDKTRTHFDIINDICREQNIKEIGFEGNEVSF
                     DLYRSMSNKLSATLKSVDLSTLRETKNEDEIKYIKKACEIVDATFYHIVDFIKIGMTE
                     KQVENEIVRIIKDLGGQKESFDTIVASGLRGALPHGKASEKIIEYGDFVTFDFGAKYN
                     NYCSDITRTICMGTINKELEEIYNIVRKANEECIRVLRPGMTTGEIDKVARDIIGSYG
                     YANNFGHNLGHGVGIMVHEYPALAPESNEVLKEGMIVTIEPGIYVPSLGGVRIEDDVL
                     ITQDGCMRLTTSTKDLIVVS"
     gene            20854..22032
                     /locus_tag="PC700_001364"
     CDS             20854..22032
                     /locus_tag="PC700_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HDF2580679.1"
                     /translation="MYNFDDKPDRVSEKCRKWDLNIIRDKFGDIREDFVPMWIADMDF
                     KIPTEIENKFIEAVRRGVFGYTYCYDEFYDSVIGWQKNMHEVNVEKEWITLTYGTVST
                     LHYVVQAFCKKGDSIILNTPVYDPFESSAKKQGVNVICNTLDVVNDRYYINFDKLEVQ
                     IKENKPKLMMFCTPHNPSGRIWTIEEMTRVATICKENNVILVADEVHAEHIHYGKFNS
                     ILKIGKELLENVILLTSPNKGFNLGGLKTSYSIVINKNIRDKFRNKLKQNSITSPNVF
                     GIIGLITAYNECHEWLSGVNEYIKSNYELLETWVNKYNKIKLMKMESSYLAWMDISGL
                     GISASEFTDKLAIDTGVLLEDGSHFVKDGEKYVRINLGTQKENVIEALNRMDLFLKSL
                     "
BASE COUNT         8934 a         2248 c         3905 g         7019 t
ORIGIN      
        1 aatttctagc attataagac tataaattga aaatattcag aaaaatacta caagaaacga
       61 tttattttgc tcttaaaata agttaaatga atgttattaa ttttgtaaat tgaaatagtt
      121 taagtataaa gttatgatat aatctaataa gggtaattct ttagattata ttaaggaaat
      181 ataggtatac aataaaaagt tatggagttg gattatggga tataaattaa ttgtaacaga
      241 tatggatgga actttattag gcaataatca taaagttaca gatgaaaata aaactgcact
      301 acaaaaagtt ataaaaagcg gtattaatgt aactttagca acaggaagag ccttcgattc
      361 agctaaatgc aatgtagatt ttcttaaaga ggacatgcca ataatagctt gtaatggttc
      421 attaataaga gagcaaaatg gaaatataat atattcaaat aagattgata caagaacatg
      481 tttaaacatt ttagatgtat tagataaata cgatatttac taccaatgta atagtataga
      541 ttctatgctt tcgaaaaaaa tagaaggacg tgaagataga ttaagtgtat ttttaggctc
      601 tgaaacagaa gtaattgtaa aagatgattt gagagaagaa atctttaaaa aagatatact
      661 aaaatttgta atcatagaag aaaaaaatcc atctattttg gatgaaataa gaaaagaatt
      721 gagaaaagtt caaggaataa agataacttc ttcatggcca aacaatattg aagtcatgaa
      781 tgaaggtgtt gataaaggta atgcagttaa aattttagct gaaaaaatga atatagacag
      841 agaagatata attgcatttg gagacaatta caatgacata gaaatgataa agtttgctgg
      901 tcttggggta gctatgggaa atgcagaaga attgattaaa caagaagctg attatgttac
      961 agatacaaat caagacagtg gagttgcaaa ggcaatttat aaattcctag agttaaaaga
     1021 atcacttagc tcttaaaaca gtatataata ggaaaaaata ggttgactat tttgtagata
     1081 gtaattataa tacaaataga taaaacataa aaatttagat tcagtgaaaa agagagtaga
     1141 tatagagaaa atctcagcga attgggggat ggtgtgagcc taatgataac actttatatt
     1201 gaaaagaact ttggagaata ctacttgaaa ttcagtatgg tagtaggtaa tctcgcctta
     1261 agagaataga gtgggtaaac attgtttacc aactagagtg gtaacacgga atatttcgtc
     1321 tcttggtctt tttgaccaag agatttttta gtttcattga gttacaagac ttataattaa
     1381 aatacaaaaa attaaaatat gaggtgaaag ttatgaaatt atggggcgga cgctttagaa
     1441 aagcggaaaa tcaacttatg gaagaattta ataaatcttt tggctatgat tgtgtactgt
     1501 acaaaaaaga tatcgaagga agtgtagcac atgttcatat gcaagtaaaa tgtggacttt
     1561 taacagaaga agaaggaaag tctataacag aaggtttaaa agggatactt gaagatgtag
     1621 aaaatggaaa gttagtattg gatggagagt atgaagatat acatagtttt actgagataa
     1681 atttaattca aagaattgga gatgttggca agaagcttca tacagcacgt agtagaaatg
     1741 accaagttgc agttgatatg agattgtatg ctaaagaaaa agcaaatgat ttggtgggtt
     1801 taatatctga atttaaagct actataaaag atgttgctga caaaaaccca gtgatgatgc
     1861 caggctatac tcatcttcaa agagctcaag tagtgacatt taagcatcac ttaatggcat
     1921 attatagtat gtttgataga gatgagaaaa gaataaaaaa tgctatagaa atattagatg
     1981 aatcaccttt aggctgtgga gctttagcag gaactacgca tgatatagat agaagtataa
     2041 cttgtgaaca attaggattt aaaaaagtag tagataattt tatggatgga gtaagtgata
     2101 gagattattt actagagcta atgagtgatt tttcaattat aatgatgcac ttaagtagat
     2161 taagtgaaga gttgatactt tggagttctc aagaatttgg atttgttgag atagatgatt
     2221 tatatacaac tggaagtagt ataatgcctc agaagaaaaa tccagatggt gcagagctta
     2281 taagaggtaa gactggcaga gtatatggaa acttatttgg tctttttaca gtaatgaaag
     2341 gaataccatt agcatataat aaagacatgc aagaagataa agaaggtttt ttcgatagtg
     2401 tacatactct tgaaatgtgt atacaaataa tggatagaat gatagccaca ctaaaggtaa
     2461 atgaggataa aatgaaacaa gcagtaaaaa atggtttttt aaatgcaaca gaagttgcgg
     2521 attatttggt taaaaataat gttgctttta gagatgcaca tggtatagta ggaagtatcg
     2581 ttatatattg tgaagataat aaaaaagcaa tagaagattt aacattagaa gaattacata
     2641 agttttcaga tgcttttaag gaagatattt atgattttat cgactatgag agtatattaa
     2701 ataagggaat aaaaaagaat ttaaaataat gtatttattt ttaaaagctg taaatttgaa
     2761 tgttatcatt taaatttaca gctttttatt gtgtaagatt ttattatata aaagtaagaa
     2821 acttaaaagg agtagtattt aaaataaata caaataaaaa attattatta aaatgataat
     2881 taatatcaat aagtattgac aaaattattt ctaaatacta ttatatataa tataaataat
     2941 aatgaaaatc aatatcaaat aaatgggagg aaatctaatg aacttattag tgattggtgg
     3001 tcatgaaaga atggaaaaag attatatggt tatggccaag aaaaaaggtt ataagacaaa
     3061 ggtatatact accatgtcat ctaaattaaa taattctata ggaagaccag atgcagttgt
     3121 catacttaca tcaactgttt cacataagat gtctagaacg gttgagtctc aagctaaaaa
     3181 acaagatata ttaatcgtaa ggcataaaaa tagtagtaag gtagcattta atgaatgtct
     3241 agatatggtt gatgaatgtc tgggaaattg ttcaaaatgt aagcttaaag acaaacaaaa
     3301 acaaaaaaat atataggtat aaaaaataca taaacataaa attaaaagta ctgagataat
     3361 agttataatt attatctcag tacttttaga tttataagga taagtatatt ataaattttt
     3421 tattataatt tccaactact tgttactttt tcatattttt tttacttcta gctttttttc
     3481 tttttttctt ctttatattt gaagctatta taaataaaac taaaagcact agaatacctg
     3541 caataatctt aacaaaagtc attaaaatat tatttccagt aaaaaatgga agactgctat
     3601 caagattatt ttttgcaatt aagtctatac tgttttctag tttaccatta ttatatacat
     3661 ttaaagtacc aaccttagct ccttttttta taggcaaatc aattttattt aactctgttt
     3721 tggtatcata attgccttga gattcatctt tttctaagaa tatcctataa ctattttttg
     3781 gttcataaat caattcatgt tgttttgtaa acaatacttt tttagtatct gtgtattttt
     3841 ctttatcaac aatagtagca cacttaaagt tatcaaatcc ataatcaatc agaatacgtg
     3901 agtctaaata taaatcagca ttagttgaat taaatacagc aacgataact cttctgccat
     3961 cttttacagc actcgataaa agacatttac cagcagcatc agtatatcca gtttttatac
     4021 catcaactat atcatatttt atatttatag gttgaccttt gtatgtaatc ttatcatgtg
     4081 atgtcaaaaa cttattagtg tttacaaagt atctctcata aggatatgct tttgtagctt
     4141 caaatttaag tgacttagtt tttacaattt gtctaaaagt atcattattc atagcttctc
     4201 tagctatgag agccatatca tgagctgttg tgtagtgatt tgggtctggt aatccatgag
     4261 gattattaaa atgagtatta gtagctccaa ttttttttgc ctcagagttc atgaggtcaa
     4321 taaatttttg tacattgcca ccaccaacat atctagccaa tacaaaagca gcatcattag
     4381 cagaatgaac caaaagagca tctagtaact gctttacagt aaaagattcc ccatttttta
     4441 gatacatact agagccatct atttgaggta aatctttaat ttctattact tgatttaaat
     4501 ctttaacctc ctttagaact aaacaagctg tccatacttt agttgttgaa gctggataaa
     4561 gcttagaatt tccatcctta ttatatagta ttttgccaga ctcatagtcc attaaaactg
     4621 catgtttagc aactatagct ggttcattat ctgcaaaaga gatattagag cttcctaaaa
     4681 atgaagtaaa tatcaataaa cagattaata aaagtgataa atttcttttc attgtgagcc
     4741 tccaaatttt aaattcatct tttagtatag catatatttg tcaataatta aaagaaagga
     4801 gtgattaata tatgaaaaaa aaggtaaaat ttatttcgtt ttttgtttta tttattttaa
     4861 taatatcatc aataatcgtt tataaatcgc ttattttcaa agatgatatt tatgttgtaa
     4921 gtaaaaaaaa tttacctaat gaaaaaacac atgttgtaga gaatgaaatt aaaggtgaag
     4981 ataataatca caataaagta actaaagaca tagataaaaa ttttcaaaat aataaaataa
     5041 ctatatacat aagtggagca gtaaatagac ctggaattgt aactatagag tcagacaaga
     5101 gactttatga tgctgtagaa ttactaggag gaacgacaaa agaagcagat ttaaatggag
     5161 tcaatctatc agtaagatta gaagatgaac aacattacat aatacctaaa ataggtgaag
     5221 caacatctgt tacaagtaat gatgattcca ataaaccaaa tcagaagaat gagtcaagag
     5281 gtgagcctaa aaataaatcc aataaaaata tatctaacga atcaaaagta aatataaatg
     5341 tagcgactat agaagagtta gattctctac ctggggttgg agaagctaca gccaataaaa
     5401 ttttacaaca cagagaagaa aatggacagt ttagttcaat tgaggaaata aaaaatgtaa
     5461 atggtattgg agataaaaag tatgaaaata taaaagattt aatttgtgta gactaaaata
     5521 taatacaaaa cttacatata gatatgttat tataaatatg tatgtatatg tgtgtacatg
     5581 tggaaattta gtaaaggagg cacttttatg aaaaaagaac aaaaaaaact tacagagatg
     5641 acaacagcag gtggttgagc cgctaaaata gggccagagg ttctggcatc agttttatct
     5701 caattgccta aaaatgataa tatagaaaat ttattagtag gactagatac agcagatgat
     5761 gctgcagttt ataaattaaa tgatgatatg gcacttatcc aaactttaga cttctttaca
     5821 ccaatggtag ataacccata cgtatttggt caaattgctg catcaaattc actatcagat
     5881 gtatatgcta tgggaggcaa acctttggtt gctatgaata ttgtttgttt tccatcatgc
     5941 catgatatgg atgtacttgc ggaaatatta aaaggtgggt ttgataaagt gaaagaaagt
     6001 ggagcacttt tggttggagg gcatactgta gatgacaaag agcctaaata tggtctttca
     6061 gtttctggga ttgtaagtcc aaataaagtt ttatcaaatg ctactgcaaa acctggtgat
     6121 aagttaatta taacaaaacc aattggagtg ggtgtattaa atacagccat gaaagaaggt
     6181 atggtagaac aacatatagc agataaagta atagaaataa tgatacattt aaataaatat
     6241 gcagcaatga gttttgagaa atttgatgta aattcggtta cagatataac tggttttggt
     6301 ctacttggtc atactttaga aatggccaaa gcttctgaag taagtattga aattgaatca
     6361 aaacatgtac caattattga aggtgcaata gaaatggcac aaatgggaat tatacctgct
     6421 ggtatgtata aaaatatgca ttatgtatct aaagatgtag aagttgtagg aaatatagaa
     6481 gttgcagttc aagatatttt atacgaccca caaacatcag gagggttgtt aatttcagta
     6541 aaagaagaac ttgcagagga acttgtaaaa gatatgaaat taaatggagc aatagaagct
     6601 aaaattatag gttctgtagt tccaaaaggg gaaaaatata ttacagtttt ataaaatatt
     6661 atagagaaag agtgattgtt ttgagcaaaa gagaattgtt tgcaatgttg ccttcagttg
     6721 atgaagtatt aagtgataat agaattgtag aaataataaa tgaatatcct agaagtttag
     6781 ttttagaatc tgtgagagaa gtaatagatt taaaaagaca atttatatta agattaaaag
     6841 aagatgcatc aaactctgta acaatagagt ttggtgaaat aatagaatct gcaatagaga
     6901 gagttaagtt aaattactca ttatctttaa aaaaagttat aaatgcaact ggtacagtta
     6961 ttcatacaaa tttagggagg tctttactta gtgaagatat aaaagatgaa ttatggtgtg
     7021 ctgcttcaag gtattcaaac ttagaatacg atttggataa tggagaaaga ggttcaagat
     7081 actctcattt aactagtaca ataaaaagac ttactggagc agaagatgta ctagttgtaa
     7141 ataataatgc agcggcagta cttcttgtac ttagcacaat ggctaaagga ggagaagcaa
     7201 ttgtatctcg tggagaatta gttgaagtag gtggttcatt taggattcca agtataatgg
     7261 ctcttagtgg tgcagaatta gttgaagttg gttcaactaa taaaactcat ttgaaagatt
     7321 acaaggaggc cattacagaa gatacaaatg tattgatgaa ggttcataca agtaattata
     7381 gaataatggg attcacagag agtgtttcta tagaagaatt ggttaattta ggtaaaaaat
     7441 ataaactacc tgtaatagaa gacttaggaa gtggagtttt tatagattta tcaaaatacg
     7501 gtttatctta tgaaccaact gtattggatt ctattcgaca aggtgcagat gtagtaactt
     7561 ttagtggaga taagatgctt ggaggtccac aagcaggtat tatagttggg aaaaaagagt
     7621 atatagaaaa aatgaagaaa aatcaactta ctagagcact tagagttgat aagcttacaa
     7681 tatgtgctct tgaagcaaca ttgagaatgt atttagatga gacaaaagct atagaaaata
     7741 tacctacttt aaaaatgttg acatataaaa ttgaagaatt agaagtaaaa gcaaataaac
     7801 tatttgaaaa aataactgct ttaaacttaa atgcaaacat aaatatagaa gatggatttt
     7861 cacaagtggg aggagggtct atgcctcttg aaactataag tacaaaggta atatcaataa
     7921 ctcctgaaca tatgaatgtt tcatctttag aaaaaaaact aagacttagt gaagcacata
     7981 taatagccag agtgtatgat aataaatatg tactagatgt aagaactata tttgatgatg
     8041 aatttgatgt aatagttgcg gaattgagaa aagcatttaa ttaaataaat ttgagctaat
     8101 aaatattaaa tttagaagta taagtatgaa gttttgatta aaaaatagct acacacactt
     8161 gtgactttag tcatagcagg tttagagggg gcaaaaaatg aaaaatgtag taattggaac
     8221 agcaggtcat attgaccacg gaaaaactac tcttataaaa gcattgactg gaagagagac
     8281 tgatacatta gatgaagaaa aaaagagagg tatttcaata aacttaggat ttacttattt
     8341 tgatttacca aacaatagaa gagctggaat tgttgatgtc ccaggacacg aaaaattcat
     8401 aaaaaacatg ttagctggag catcaggaat tgatatagtt ttacttgttg tagcagcaga
     8461 tgaaggggtt atgccacaga cagttgagca tttagatatt ctaagctttt taaatatcaa
     8521 aaatggaata gtggttctaa ctaagtctga tactgtagat gatgaattta gagagctagt
     8581 aaaagaagat atttgtgaaa aaataaaagg aacttttctt gaagatgctg aaataattga
     8641 agttgattct atatctaaaa gaggtataga cactcttatt gaaaaaatag ataaaatgag
     8701 tgatgatata gaagataaaa atgaaaactc tccagcaaga cttaatatag acagagtatt
     8761 ttcaataaaa gggtttggta ctgtaataac aggcacttta atagaaggaa agataagtat
     8821 agaagatgat ttagttatat atccacaggt attaaaaaca aaaataagaa gtatacaagt
     8881 tcatggagaa aataaagata cagcttatgc aggtcaaaga acagcaatca atatatcaaa
     8941 tgtaaaagtg gatgatataa aaagaggaga tgttttagct gcaccaaact cattagagga
     9001 atcaatgatg ttagatgtaa aattatcatt ggtaaatcat ttagataaag gattaaagca
     9061 ctgggataga ttaagattgt atcatggaac tagagagata ctttgtagag cagttccact
     9121 agataaagaa ttaattgaaa gtggagaaag tggctatgtt cagttgagac ttgaagaaag
     9181 catagtatct aagaaaggtg atacgtttgt agtgagacgt tattctccaa tggaaactat
     9241 aggtggaggt gtaataattg acccatctcc taaaaaacat aagaaatttg atgaaaaagt
     9301 tatagaagca ttaaaaataa aagaaaaagg cgaactaaaa gatataatag aagagtactt
     9361 aaaaaggaat ttaaaaaatt atccaaatat aaaagaaata atgagctata gtggtgctca
     9421 tgaagaagat gtaaaaagag cgcttgaagc acttatatca gaagataaag tatttattat
     9481 aggaaatatg tatatgcata taaatcaata caataaatta aaagaaaata caataaagct
     9541 tttaagtgag taccataaga agtataggct tagaaaaggg atactaaaag aagaagtaag
     9601 gtctaagata gagagtaatt ttaagactag ggagatggat attttattag aaaaattgtc
     9661 tacagaaaat gctataaaaa tagaaaataa tatagtatct ctattagatt ttgaggttat
     9721 tttaaatgat aagcaaaaag aaatagcaaa aaaaatagaa aaaagattaa aatcatgtgg
     9781 agtttcttct atactgacaa tagatgaagt ttctgaagga aatcataact atgctgaagt
     9841 attggagtca atgataggta ataaagtaga gaaattagat gatttgtata taatggacaa
     9901 agatatttat gaaaatgcaa agaacatttt gataaattat ataaaagaaa ataaagaaat
     9961 aacattagga gaatacagag atttgattga ttctagcaga aagaattgca tgattatatt
    10021 ggaaaacttc gatagaaaca aaataactaa aagagtagaa aacaaaagaa ttttgtttta
    10081 gcagaaaatg tctgtttttg ctttaaggta taaagaagga attataatgt cgagtatcgt
    10141 ataattaatt agtataaaat attaattaag aggaagtgta aagtattgaa gaatataata
    10201 cctgatgtaa aaccagatat aaaagaaaga gaagacatga gccttttatt tataataatg
    10261 ggctcagtac ttttattatt tattataaaa gatatagaga aatatgaaac tttattacat
    10321 gccatatata cgatattttt agtagctaca ggagtaatta tatttaatac tacaaaattt
    10381 aaagtagatt cattttctac atttttagga ttaattttta tagctactgg agtattggag
    10441 tgtatatatt tatttaattc cttggggatt aaaactaagt ctataatgga gattaacatc
    10501 acaatatcag caataacaga tttatttcct atactgggtg tatacttaag ttttaaattt
    10561 gttaaggata acaaacaaat ttattcaagt ataattttat ttatagcaac tgctacatta
    10621 acaataacat ctttattcat aatatcagga atttgtgact atctgggaaa tggtaaatca
    10681 caatatgctt taggagttat aatatctata ttcataataa ttatttctat cattagtgga
    10741 attgaattaa attcaagttc aaataaaggt aaatgggaat atgatgaaaa aaagattttt
    10801 aacagaataa tagtaataat aattttatca agagttccaa atctattgca tatagttatt
    10861 gataatagaa atgtagagcg gatattatca cagataataa taaatgtagc cttatattat
    10921 ttatataatt acatagtatc taagaatatt aagaaaacag cactggaatt gcatgatacg
    10981 aatgaagaat taacaaaaaa aacggaaagt ttaaaagaaa agaataaaaa attaatctat
    11041 gagacagaaa agatagagga acttaaggga atacttatga aaagagaatc taggctacaa
    11101 tctacattag atgtagcagt aaatagcata gtagtgttta gcaaagatgg agaaattaca
    11161 tatgcaaata agagctttag aaatactttt ggagactata aagaacaaga taaattgact
    11221 gataaaataa aaaacttcaa tgatttgata gaaagcattc attatgtgtt tgttcatgag
    11281 aagaatgcag agaaacttat tagtacaagc gataataaag tttatcaagc tatatttgca
    11341 cctttaatta ttgcttcaca aacagaagga gtattgtgtg tactagaaga taagactaag
    11401 aaaaaggagt acgaacgcaa acttattgaa gcaaataaga gaagtaaaga ttttttagaa
    11461 agtattggag atggcatagt agtgcttgaa ggtaataaca aaatatatgt taataatgca
    11521 tgtagggaaa tttttaaaaa taatttagag agtattgatt tttcattagt atgtaaaagt
    11581 gaagaaagta tagaaaaaag atatgtaata gatggtgaag taaagtatgt agagatgagt
    11641 ttttctcagt atactaatgc tggtactaat aaaactatta ttgtaatacg tgatacaacc
    11701 tctcgaaaaa tatctcaaat aaaacttgag gaaagtcaat catcatattc aagatttata
    11761 gatatattac ctgatggcat atgcttatta aaagaagatt taagtataaa ctatgcaaat
    11821 aagtcgtttt tagatatgct agcttttaca aacatagatg atataaaaga ttctaatatt
    11881 aaactattaa tgaatgcgaa tactgaagaa aaaatgaaat ttacggataa tatggctaaa
    11941 gttttaaatg aaaatagaag tatactttta ctggaatatg aattgataaa tagttatgat
    12001 gatatagtag aagtagaatt aagtgcatta ccttttgcta tatacaatac tagatacata
    12061 atgcttatta taaaagattt agtacataag aaatattccg agcaagcaga aaaagaactg
    12121 cttgaaagat ttaaaacaga taaaatcaaa acggagtttt ttgcaaatat gtctcatgaa
    12181 ttaaaaactc cactaaatgt tatatctagt tcaaatcaac tggtagattc tttctataga
    12241 aatgaaaaga taaaagatta caataacaat attaaatctc atgtggattt agttagacaa
    12301 agttcttata ggcttcaaag attaataaat aacattatag atttaacaaa gatggagtct
    12361 gggttctata cactaaaact tgcgaaatac aatattgtat ctgtaataga agacttattt
    12421 atgaatatag aagagtatgc acttagaaaa gacataaaaa ttctttttga tacagacctt
    12481 gaagaaatta atgtttatat agacaaggtt gaaattgaga gaattatgtt gaatctattg
    12541 tcaaactgta ttaagtttac tgataatgga ggctggatat atgtaagtat tcattataaa
    12601 atagataaag taatcataag tgtaaaagac actggagttg gaataccaca agataaatta
    12661 gaattgattt ttgaagaatt ttcacaagtt gataagactt tatccagaaa tactgaagga
    12721 agtggaatag ggcttgctat agttaagaat ctagtaagtc ttcatggtgg agatatagaa
    12781 gtagtaagtg aagtgaataa aggcacagag ttcttaattt cacttccaat gaagggattt
    12841 agtagtgaac attacacaga agataagaga atatataata ttcaagaaaa aataaaaata
    12901 gaattttctg atatatacta ttaaaaatga ttatgagcaa aaataaaata aaaatactat
    12961 ttgtttaaat atttaagttg taagaaatag gctatcataa ggtaaattat tatgatagcc
    13021 tatttttagc taggttttat atctatatgt gagaacatat aatctctaaa ttaccttcaa
    13081 aactaaaatc tccaaagtcg tttggcagta taaaatgcat accctttttt aaattatatt
    13141 ctacagaatt gtgtataagt tttccatttc catccaacac actgtataaa gaaaaaggtg
    13201 tattgtgtgt aaaattacat tttccaaaaa catctaattt ataaacacta aaaaattcgt
    13261 ttgacacaaa tgttgtgcat ttaaagtctg atgttgatac gattttatag tcagtatcga
    13321 aatttatatg aggaacattc gtaacatcta ttgatttttg aacatgaagt tcacgcttat
    13381 tacctgaatt gtcagttcta tcataatcat agactctata agtagtatct gagttttgtt
    13441 gagtttctaa aatcaatgtt cctttgcata tagcatgtat agtaccactt ggaacataga
    13501 aaaagtctcc cttttttata ttaacctttc taagtagttt gtcccattca ttattattta
    13561 ccatatttac aaattcttta tgagattttg cattatgacc tattataatt tctgcatcat
    13621 cagaacaatc tattacatac caacactctg tcttacctaa ctctccattt tcatttttct
    13681 tagcatattc atcatttgga tggacttgaa ctgataaatt atcatttgca tccaatattt
    13741 ttgtaagtaa aggaaactta tcaccaggtg tgtttccaaa taattctcta tttttattcc
    13801 acaattcaga tagtttttta cctttatatt ttccattttc tattaagcaa tctccatttt
    13861 tatgagagct tatagcccaa cattcaccag ttgttggcga atctatttca taattaaatt
    13921 tatctttaag tgcagtacca ccccatattc tatccataaa aataggtttt aaaaataatg
    13981 gttccataat cctgttcctc ctatgatatt aaatttatcc tacaaataat tatatataaa
    14041 tagattataa aatcaatagt aaaaaagttt tgtcccttga aatgacataa agtataagga
    14101 gttattaaat aagataaact atgatatata atataaagtt ctaaattgga gtgataatta
    14161 tggagttgtt taaggcagaa agcaaaaaat atgaaaaaga atttttaaaa ttattggagc
    14221 aatgggttag tataccttca ttttatgata gaaaaactgt atctaaagat atgccttttg
    14281 gaaaaggagt atatgatgca ttaaactggt ttgaaaatct aggtagagaa aacaatttca
    14341 aagtaaaaaa tatagataat catgctgttc aaattgaata tggaaatggt aaagaatatg
    14401 tagatatatt tggacattgt gatgttgtaa atccaggtga aggatgggat agtgaaccat
    14461 ttaaactaaa tattataggt gataaattag ttgctagagg tgtatctgat aataaaggac
    14521 ctatgatagt gaatttttta gcactaaaaa tgataaagga tttagatatt aatttaaaaa
    14581 gaaaggttag attaatagct ggaggaaatg aagagagtgg atttaaatgt ataaagcatt
    14641 attatagcaa agagccatat ggtgtatgtg gatttactcc agatgctaaa tttcctgtat
    14701 taaatggaga aaaaggtgga gcaataataa agttaatttc aaatatagac gataaatctt
    14761 tatatataag tggtggaata gagtttaata caataccaga taaagtttat ataaagaatg
    14821 tagaaaaatt gggtaaagat aatatatgtt ttgatattaa caatatatcg ataaattatg
    14881 ataatggcaa ttatattgtg tatggaaaag gtggtcattc atctaaaccc gaaaaatcaa
    14941 taaatcctat attagctaca attaaacttt tgtcagaaaa tattgatgaa aaatggacaa
    15001 aagatttata taagttgata aatcaagata atataaatgg aaacttattt ggtttaaata
    15061 ttgaggggaa atgtgggatt ttatcaatag tgccaacaat aataaatata gtagatggaa
    15121 aattagaagt agttttaagc gttagatatc cagaaatatt aaccattgaa gatataataa
    15181 aaaaatttaa tttgtatatg gagcaaaata atattaataa atttgagctt attggggaaa
    15241 atttaaagca agcaaattat atagatagaa attcaaaact tgtaagaagt ttgcatgata
    15301 tatatataaa gtattctgga gatttgaaaa atgatgttag agtaactagt gcaggaagtt
    15361 atgcttctga aatgaataat tcagtaatat ttggttgtga atttcctgat ggtagctttg
    15421 gcaatgtcca tagtgcgaat gaatttgcta gcttagatag atttataact gcaataggta
    15481 tatatgcaga ggcaataatt acattatgta ataaaatata aatacaaaat gtcccttata
    15541 tagacatatt atatatacaa ttaaaatttt acttgatata taattgaaat caaagagaag
    15601 gtgatacaat gataactaag aaacatgcaa taatagtaaa gagtttaagc aataaagatg
    15661 gatatatgac atcaaacgaa ttagctatca aattggatgt gtctactaaa actattaaaa
    15721 gatatatagc agatttaaat agtgttttaa gcaaatatga tttggtaata gcttcttcta
    15781 gaggtatagg atacaagtta agcggttcta aaaacaatat agccagagct gttaaagaag
    15841 caaataaata tattgatggt tttttagatg actctgaaga gtcaagaatg tcaaatataa
    15901 tatgtatgct tattaataga aattatatga gtatagaagc aatggcagaa gaattaaatc
    15961 taagtatagc cgctataaat aaattgtcta gtaagcttaa aagaaaactt gaaaagtatg
    16021 acttagttat aaaatctaaa ccttactatg gaagctatat acatggtgaa gaaattaata
    16081 taaggcaatt aattactgac tatgcaataa aattagatga aaaaagtaaa gtgaaggtgt
    16141 ttttagatga cattagtgaa aacgatatat actgtataga aagtatttta gaaaagcact
    16201 taagagaaaa agatacaata atttcagata aagattttaa tcttttgctt tcaaaaataa
    16261 ttgtttctgt atttaggagt aagagaggtc atagtaacaa tataaactta atggatacaa
    16321 gttatagatt tcataactac tactttatag aaaatctaat gaaagaaata tctgataaaa
    16381 ttggatttaa acttattgaa gatgaagtaa tttatatttc aaattattct ggtgtaatag
    16441 cttataaagg tacacaagga agaaaaaaca caagtgaaat tgaggaacga ataagtagtg
    16501 ttataagtag tgcacttcaa gatatattcc ttatttcagg tagtgattat acaaaagatg
    16561 atgagtttat ggttgcaatc tctgaccata taaagagatt tttaaataga gctagagcta
    16621 atgtaaaatc aaataatcct ttacttcatc aaataaaaga gaagtttcct atagctttta
    16681 atttagccgt gtttttatca aataaactag aaactgagtt taatttaaaa cttgatgaag
    16741 atgagcttgg atatatagct atccactttg ctgcttcaaa tgaaagaatg aaaaaaaata
    16801 ctagtaagaa aatttgtata gtttgtcatt atgggatagg aacaggacaa cttatttctg
    16861 aaaagctaaa acagaatata agtgatttaa gtgttgtagg tgtttatcca gttagatatc
    16921 ttgatatggc tataagtcaa gatgttgatt tgatagtatc taccgttgaa ttaaaaggat
    16981 atgaaaaacc tgtcttatat atagaaaata tatttgatga tagcttaatt gaaaacgtaa
    17041 acaaagcctt ttatgaaaag gaagaaagaa gaaagataat aagtaacatg tttgatgaaa
    17101 aagctttttt cagtataaag gctagtacta aagaagaggt cataatttca ctttcaaata
    17161 aactaaaaga aagagatttt atagaggaaa gttctataaa aagtataata gatagagaaa
    17221 atatttcatc aactgaaata ggaaatctag tagcaatacc tcacacaata gtgaagggag
    17281 ataaaaaatc tataatagga gttggaatac ttgaaaatcc aataatttgg gataagcaag
    17341 aggttcagct agtatttatg gtatttttta atactaaaga aaagcataat ttctcgatat
    17401 ttaaatacct ttataatttt ataaaagatg aaggaggagt aagaggaatt attaaaatat
    17461 gtgactttaa taaattaatg gcactgatag gtaattaagg aggaaattaa taatgggaac
    17521 aaacatattt aacaaagaat atgtattttt aaatgttgat gctaagtcaa aggttgaagt
    17581 tttaaaattt atatcaaaaa aagctaaaga cttaaatttg gcagaagatg agagcttagt
    17641 atatgaaggt ttaatggcta gagaagaaca atttactaca aatttaggag aatctatagc
    17701 aatacctcac actaaaaatg atgcaattga aaatccagca gtagtagtat tgaagtttaa
    17761 tgaagatgtt gtttggaatg aaggtgagga taaggttaaa ttagcaataa gcttacttat
    17821 gccaggaaaa agtaaagaga atattcatct taaattatta tcatctcttt caagaaaatt
    17881 aataaataaa gaatttaaag attctctttt aaaaagtgat aatgttgaag aaatatctaa
    17941 cttaataaat gaagctttag gtttataatt aaatactaag gttataaaat aaaaaaataa
    18001 ttaactaaaa ataggaggaa tttgaaatga ataaaaaatt agtagcatta tgtgcttgcc
    18061 caatgggact agctcacact ttcatggcag cagaagcaat agagcaagca gcaaaggcat
    18121 taggttatga agcaaaagtt gaaactcaag gtgctgatgg tgtacaaaat gagttaacaa
    18181 gagatgatat attaggagca actatgataa tacatgcagt agcaataaca ccagaaggta
    18241 tggaaagatt tgatggttgt gaagtttatg aagttgaatt acaagaagct ataaaaaatg
    18301 cagaaggtgt tataaaagaa atagaagaag atttaggaat ataataaatt tattgtggag
    18361 gaaatagaat ggcaattaag aaaaaagtta taaacagttc aggtgcttct acaacaggag
    18421 gaaataatgt aggaaaatct actccatcta gtccaactca atcaaaggga aatggaaaat
    18481 ggaaagaaat aagtaaacat ataatgacag gtatatcata tatgatacca gttttagtaa
    18541 tgggtggact tataggggcc ttatctcaat taataccata tgctatatta ggacttgacc
    18601 catctgtagg tatagttgat gctatgaact caggagaatt tacagggttc aagcttagtc
    18661 ttttaaatat agcacaatta atgtcaaatt tcggatttac tttgtttgga tttgcgatac
    18721 cactgtttgc agcattttgt gcaaactcta taggtggaaa aacagcttta attgcaggat
    18781 ttataggtgg atatatagct aataaacctg taggtgtacc acaattcatt gatggacaat
    18841 ggactgaagt tgttccagtt gcatcaggat ttttaggtgc aatactaata gcattcataa
    18901 taggatattt tgtaaaatgg ttaaataaat ctataaaagt ttctcataac tggttagctt
    18961 ttaaaacaac atttttaata ccattaatag cgtcattagc ttgtatggta ttgatgatat
    19021 ttataataac tccatttggt ggattaatta atgaaagtat gaaaaacttc ttaacagcag
    19081 caggagcagc tggagaatat gtatatgcaa cagctttggc agcagctaca gcattcgact
    19141 taggaggacc aataaataaa gcagcaggat tcgttgcact tggtttaact acagagaatg
    19201 tacttccaat aacagcaaga acaatagcta tagttgttcc tccaattgga ttaggattaa
    19261 caacattatt agataaaaga ttagtaggaa gaagagtata tgatagacaa ttctatcaag
    19321 caggaaaaac ttctatattc ttatcattta tgggtatatc tgaaggagca ataccatttg
    19381 cacttgagag accaggtttt gttatacctt taaacatagt tggaagtgta ataggagcta
    19441 ttacaggtat aattctagga gcaattcaat ggttcccaga gtcagctata tgggcatggc
    19501 ctctagtaga taacttattt ggatatataa taggtatagc agttggtgct atattcatag
    19561 cagtaggaaa tatattctat agaaataaat taataaaaga tggtaaactt gttgtagatt
    19621 atatcgacta gtatttaatt tttactatct ataaataact ttggctggta atatggtttt
    19681 aatcaatatt accagcctta cctatatgat taattaaagg cggtggagat aagatatgga
    19741 taagatagaa aaagtaagag agtattttag agaatatgat atagatggat ttcttataaa
    19801 tagtcctaca aacaagtttt atgttggaaa tttatttagc tcttcaggtt atgtttttat
    19861 aacgaaagaa tcacaatata taatagtaga ttttagatat tttgaagaaa ttaaaagaaa
    19921 atctagtttg tttaatgtag tattaatgga caaaactaga actcattttg atataattaa
    19981 tgatatttgt agagagcaga atataaagga aattggattt gagggaaatg aagtatcttt
    20041 tgatttatat agaagtatga gtaataagtt aagtgcaacc ttaaaatcag ttgatttatc
    20101 tacattaagg gaaacaaaaa atgaagacga aataaaatat ataaaaaaag cttgtgaaat
    20161 agttgatgct acattttatc atatagtaga ctttataaag attggtatga ctgaaaagca
    20221 agttgaaaat gaaattgtta gaattataaa agatttaggt ggtcaaaaag aatctttcga
    20281 tacaatagtt gcatctgggc ttagaggagc attacctcat ggaaaagcaa gtgaaaaaat
    20341 tatagagtat ggagattttg ttacttttga ctttggtgct aagtataata attattgttc
    20401 agatattacc agaaccattt gtatgggtac tataaataaa gagttagaag aaatatataa
    20461 tatagtaaga aaagctaatg aagagtgtat aagagtttta agaccgggaa tgactactgg
    20521 agagatagat aaagttgcaa gagacataat tggttcatat ggatatgcca ataattttgg
    20581 tcataatctg ggtcatggtg taggaattat ggttcatgaa tatcctgcct tagctcctga
    20641 atcaaatgaa gtattaaaag aaggaatgat tgtaactata gagcctggta tatatgttcc
    20701 aagtcttgga ggcgtaagaa tagaagatga tgtactcata acacaagatg gatgtatgag
    20761 attgactaca tctactaagg atttaatagt tgtaagctaa aaataaatta gataaatatt
    20821 ttataacaat aagaaaagaa gaggtaaatt taaatgtata attttgatga taaaccagat
    20881 agagtaagtg aaaagtgtag aaaatgggat ttaaatatta taagagataa atttggagat
    20941 attagagaag attttgtacc aatgtggatt gctgatatgg attttaaaat accaacagaa
    21001 atagaaaaca aatttattga agctgtaaga agaggtgtat ttggatatac atactgttat
    21061 gatgaatttt atgattcagt tattggttgg caaaaaaata tgcatgaggt aaatgtagaa
    21121 aaagaatgga ttacattaac ttatggaact gtatcaacac ttcattatgt agtacaagct
    21181 ttttgtaaga aaggtgatag tataatatta aacacaccag tttatgaccc ctttgaatca
    21241 tcagcaaaaa aacaaggtgt aaatgtaatt tgtaatacat tagatgttgt aaatgataga
    21301 tattatataa attttgataa attagaagtt caaattaaag aaaataagcc taaattaatg
    21361 atgttttgta caccacataa tccatctgga aggatatgga caattgaaga aatgacaagg
    21421 gtagcaacta tatgtaaaga aaataatgtt attttggttg cagatgaagt acatgcagag
    21481 catattcatt atggtaaatt taattcaata cttaagattg gaaaagagct tttagaaaat
    21541 gttattctac tgacatcacc aaataagggc tttaatcttg gtggattaaa gacttcttat
    21601 tcaatagtta taaataagaa tataagggat aaatttagaa ataaattaaa gcaaaattct
    21661 ataacttctc caaatgtgtt tggaataata ggattgataa ccgcttataa tgaatgtcat
    21721 gagtggttaa gtggtgtaaa tgaatatata aaatcaaatt atgaactttt agaaacttgg
    21781 gtaaataaat ataataagat aaaattgatg aaaatggaat catcttactt agcttggatg
    21841 gatataagtg gattaggtat aagtgcaagt gagtttacag ataaattggc tatagataca
    21901 ggagtattat tagaagatgg gagtcatttt gtaaaagatg gtgagaagta tgttagaata
    21961 aatttaggaa ctcaaaagga aaatgtaatt gaagctttaa atagaatgga tttattttta
    22021 aagtctttat aaaaatttgg ttgaattagt attttatata aatttaagct attaatatgg
    22081 tatatacata agtagaattt gtttta
//
LOCUS       DAMJWP010000041        21676 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.042, whole genome shotgun sequence.
ACCESSION   DAMJWP010000041 DAMJWP010000000
VERSION     DAMJWP010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21676
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.042"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(355..1446)
                     /locus_tag="PC700_001365"
     CDS             complement(355..1446)
                     /locus_tag="PC700_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580680.1"
                     /translation="MVMQVQEKILELLYTINEDVVSIKNDMSEVKQEMTVMKEDISEV
                     KQEMTVMKEDMSEVKQEINVLKGDMSEVKQEMTVMKEDMSEVKQEINVLKEDMSEVKQ
                     EMIVMKEDMSEVKQEMTVMKEDMSEVKQEMTVMKEDMSEVRQEINIMKEDISEVRQEM
                     TVMKEDMSEVKQEINVLKEDMSEVRQEINIMKEDMSEVRQEMTVMKEDTSEVKQEINV
                     MKKDMSEVRQEITVMKEDVSEVKSEVNFMQNKINNINEDMNGIKDEVSIANEKLYGIE
                     IKIDSLECEDKSMKEIQIEQNNILGSLLHNSEINKATHDNIEHNIAYIKGDTNSIKED
                     IAEIRRDLNLVELATSKNWSDIVKLKSVK"
     gene            complement(1676..2761)
                     /locus_tag="PC700_001366"
     CDS             complement(1676..2761)
                     /locus_tag="PC700_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDF2580681.1"
                     /translation="MYNFLGKELKSPLIIGSGPLTYSAAGCKILSDAGAGAVVTKTIR
                     KERAINPAPHMVRNTANALLNNEKWTDFEPEQWIDFEIPQMKRDGTVCIASVGHTIEE
                     SSELVEKVANAGADFIELVSYDYRDLIPMLKDAKERVNIPVIVKLPPMIDEIGDFAKK
                     LEEAGADAITACDSVGPAFRIDIETGQPLLGGNGIGYLSGETIKPITLQRIYEIKKQV
                     NIPIIGLGGCVSGDDALEMIMAGADFVGICSVVILKGAQVISKIHDDLKSNLNRLGYN
                     TIENACGIVHNHMGDVNERLSFEFTYDEEKCTKCKMCERVCAYQARKLNDKMELNEEE
                     CRYCGLCISVCKPKALGRKISCSELNV"
     gene            complement(2797..4110)
                     /locus_tag="PC700_001367"
     CDS             complement(2797..4110)
                     /locus_tag="PC700_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF2580682.1"
                     /translation="MIAIKSKYLISSADEIYTNSAAVVENDIIKDILPNEEVEIKYKN
                     IEEIIDKSDAIIMPGFVNGHMHQYGVLSRGIPANVHFTDFEGFLNDYWWPFIENRIGL
                     KEVKATTKASAIELIESGVVAFCDTLEAPNTEEGTLIEQGKILEEIGMKAILSLESCE
                     RISFENGLRCLDENSNLIKWSRENSKLINGIMCTHTSFTCSDRFIKKAKEDAKKLNAP
                     MQFHLCESIYEPNYAEKHFGKKAVDYYNDLDILDETVLASQCVKVNDEEIDILKEKGV
                     KVVHMPLSNCEVGGGFSPVPKMIKKGIKVALGTDGYINDFFEVMRGAFLMHKSVEEDA
                     SVMPANLVFRMATEHGAYVLGLQNSGKIAVGNKADIIVMEDEFKTPVTLDNIFDQIVV
                     QGKKEFISNVYIDGRHILKEKQLVDLDKKAIVKEMKEVACEFWKF"
     gene            complement(4111..6402)
                     /locus_tag="PC700_001368"
     CDS             complement(4111..6402)
                     /locus_tag="PC700_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2580683.1"
                     /translation="MVNKEVRKIDSEAIVTGKPIYTEDLIFHKDVLTIKLLRSPHAFA
                     KIKNIDTKNALKVKGVVNIFTYKDVPNYRYSMVGESYPEASPHDQLILEDVVRYVGDE
                     VAIIAAEDERAAIKAMKLIKVEYEVMTPILDARESEGNKILIHEEDDLFCPFDFGLDA
                     TKNIVSREKMGKGDVEKEFKESDVIVEHSYMTQSQAHCMMETHRAYTYIDTNGRLVVT
                     SANQSAYHMRRQVSKALGIPLSKVRVIKPRIGGGFGGKNIAVTEIYASFVTWMTKRPA
                     KLIYTREETFAMTNTRHQMFFDIKIGADKEGNIKAIDMKALNNTGAYGDNGPSVCSES
                     GHNVLPTYNNVPAIKFDGRTVYTNLVPGGALRGYGATQGTFALDCAIDELAHKLNMDP
                     TEIKLKNIIKKQTEGGIMNFPIRSCALDECIIRGKKLIGWDEKYPVRDIGNNKVRAVG
                     MSVGTHGSGIANIDMAIVNMRMDEDGTYKLFSGSSDLGTGSDTILAQIAAKALNTSID
                     RISVYTGDTDMCPYDTGAYASCTTYVTGNATIKSAESLKQKILKVAQKKLDLPIDDLI
                     LYEDRVCYSEDVEKFVLLSELGKESVGGGNQEVLYSSESYGIQESAKPFLAGFAEIEL
                     DKTTGEFKVINYACSTDCGTVMNPALARIQVEGGVTQGIGLAMYEDPRHGSDGKLQTS
                     NLLQYKVPTKKDIGNIIVDFVDNYEPTGPYGAKSLGEIVIHTPAPAIANAIYNATGVR
                     IRELPITSEKVYMAMKKLNQGGK"
     gene            complement(6392..7186)
                     /locus_tag="PC700_001369"
     CDS             complement(6392..7186)
                     /locus_tag="PC700_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HDF2580684.1"
                     /translation="MITIKEYVVPKSLDEAYELLISRKNNIILGGCGFLKLGSKNIGS
                     AIDLKDLALDYINETDDSILIGADTSLRTLELNKVIKNYCNGVISNAVSNIVGVQFRS
                     GARVGASVFAKYGFSDLIPSLLVVDAKVKLYKKGVMNLADFLESELEKDILIEVILPK
                     KDAIGVFDSIRKCTGDFAVLNGAMLKEGSIYKIAIGARPRRAKIAYKASEILSLENDI
                     EKAGEVSSEELSFGSNIRGSKEYRKDMAKALVVRMYNSIGGECDGK"
     gene            complement(7225..7671)
                     /locus_tag="PC700_001370"
     CDS             complement(7225..7671)
                     /locus_tag="PC700_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HDF2580685.1"
                     /translation="MIIKCTINNKEKELSIRPDDFLADTLRENGYVSVKKGCDTGSCG
                     LCTVMVNDKAVLSCNYLTARVDGQHITTLEGVLDEAKKFGDFLADEGADQCGFCAPGF
                     IMMVLAMKRELQNPTEEEVIDYVNGNLCRCTGYKGQLRAIHKYLNT"
     gene            complement(7743..9149)
                     /locus_tag="PC700_001371"
     CDS             complement(7743..9149)
                     /locus_tag="PC700_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2580686.1"
                     /translation="MMDILIKNAIIVTVNKEREVIFDGALVVKDNKIADIGNSKEIES
                     KYTDVKKIIDAKGKVLFPGFINTHNHLFQTLLKGLGDDMVLKDWLETMTFPAANYLEP
                     KDTYDAAMLGCIEGLRSGITTMVDYMYPHSKPGLCDGIIDAYKELGIRGILGRGCMNT
                     GAQFGVHPGIMQDVETVEKDVRRLFEKHHNTENGRIKIGVAPAAIWSNSQEMLEMLWR
                     VVKEYDDALFTVHISETPFDREAAKELHGQYDIDVLEKLGILGPNVLMVHCVYLTEKD
                     MELTKKYDMKVSHNTASNMYLSSGVAPVPEMLKKGITVSLGVDGAASNNSQDMLELMK
                     LTALQHKVNKCDPLAMSAEKVLELATIDGARAIGMEDEIGSLEIGKKADLLIFNPMLS
                     PKAIPMHNPVSTLVYSSSMKNIESVIVDGNIIMEDSKILTANEEKALKDAQDTAERLC
                     VRGTIKNRMEGHKWNSLY"
     gene            complement(9167..10537)
                     /gene="hydA"
                     /locus_tag="PC700_001372"
     CDS             complement(9167..10537)
                     /gene="hydA"
                     /locus_tag="PC700_001372"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HDF2580687.1"
                     /translation="MGTILRGGTIITSDKTYISDLRIEDEKIVEIGNNLEINGDKVID
                     ATGKIVIPGGIDTHTHFDMNAGSITTADNFETGTRAAIAGGTTTILDFAEANEGENLL
                     QGVEAYHKKASGNCYCDYGFHMTITCLNKDTFNHMEKLISDGIVSFKMYMAYDGMKVD
                     DGTIYRVLKKARDLGCIVGFHCENGDLLDVLIEENIQNGNLEPKYHPLTRPNIVEKES
                     VSRLADITKLSNSKSYVVHLSCRESLETVKMAREKNIDMIVETCPQYLLLEDNLYNKN
                     KFEGAKYVMSPPLRKKEDINYLWEGLSSGDIQTVGTDHCSFNFKGQKDLGIDDFSKIP
                     NGAPGVEHRLALLYTYGVLENRISVNKFVEVTSTNAAKIFGMYPKKGEIAVGSDADIV
                     ILNINKEETISYKTQKQNVDYTPYEGFKVKCKVEDVFLRGNHVVQSCNVKEHPTGQYI
                     KRKITK"
     gene            complement(10670..11857)
                     /locus_tag="PC700_001373"
     CDS             complement(10670..11857)
                     /locus_tag="PC700_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HDF2580688.1"
                     /translation="MLNEQRKQEVTEICQKLIQNPSSSGNEEGVVKAIEESFEKLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEGKIVDGKIYGRG
                     TSDMKGQYTAMMSAVAYFAKDTNRDFAGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKGVNAVYKMANVIQKIQQLVPPT
                     HPVLGDGILVLTDIKSSPYPGASVVPDYCKATFDRRLLVGETREGVLAPIQALLDEMM
                     KEDPELNAKVSYAVEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDSE
                     ITQYSFCTNGSHYAGEAGIKTIGFGPSKENLAHTIDEYIEQEQLFIGTEGYYGILKSV
                     YGK"
     gene            complement(11984..13198)
                     /gene="dpaL"
                     /locus_tag="PC700_001374"
     CDS             complement(11984..13198)
                     /gene="dpaL"
                     /locus_tag="PC700_001374"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HDF2580689.1"
                     /translation="MLKEIKWKVNNLPKGDKENCIKFLNEEEITKVRNFHKSFPQYKE
                     TPLANLEGLAKKLGVAGVYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAQEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNHAAAFVSAPDWVSAKGMRTLGNP
                     LNGDEKVISGESGAVTTGLLVAAMEREDLADLRKDLKLDENSRILLISTEGDTDPDKY
                     RSIVWDGEYPSI"
     gene            complement(13561..14232)
                     /locus_tag="PC700_001375"
     CDS             complement(13561..14232)
                     /locus_tag="PC700_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2580690.1"
                     /translation="MAKILGICGSPRKGATEYAVLEALKEAETIPGIETEYWSVRGKK
                     ISPCVHCDACIRKETMCIIKDDIQELEQKILEADGIIVGSPVYDMNITAQLTAVFNRL
                     RPMYLVHPGKLQNKVGAAITTGGTRYGGQELTKLPIINFFLMHEMLVSGGLGGCYIGG
                     TIWSKDGKAKGAQEDETGMDTVKRLGKGAAEAVMVSKFGLEKWNVVKEELDVKKDEKS
                     PLRDH"
     gene            complement(14315..16531)
                     /locus_tag="PC700_001376"
     CDS             complement(14315..16531)
                     /locus_tag="PC700_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2580691.1"
                     /translation="MEKVIGKSYPLKDAALKVTGQMKYVADMKPQNVLYAKMLLSPVA
                     HAKIKSIDTSEAEKVVGVRAIATYLNTSQRKYNGSLRFYEHNIPENEVIFSDTVRFVG
                     DRVAAVAAETPEIAEKAVKLIKVEYEELPAVFTIQDAIKEDAICIHGDSNIVSQNNIN
                     GGDVEKGFKECDYIFEDEYSTPAIHHSAIETHSVIAHYDYNDKLTVHTPCQNTFGFRI
                     ILSQIFGMSLNKVRVIRPAIGGSFGGKLELTIEPVAAALSKMTRRPVKMVLTRKESMI
                     STRTRHGSFVKLKTGVNKDGEVIAQDIKIYTNTGAYASSALNVIGALSHKVFKVYKIP
                     NIKFTGMPVYTNTPIAGAMRGYGSPQIFMAQQAQFAKIAKEIGMDLVDFQNKNAVEPD
                     DVDIIFRGSLGNPRVLDCIEQGKKMFKWDEKKKLPKEEGKYLRGIGMAIGAHGNGVFG
                     AHRDVVTLTLKLNEDGTLTLLTGAHDMGNGVVTMQTMMVAEVLGITPDKVDTFETDTD
                     ACTFNLGDYASRGVFVEGGGAKKTAEKLKNLIVEEAEKLLETSKEDLYLDNGYVISKL
                     DENVKASLSDVAVYSQSKSLRELTVVEDYSSPAGLTSYGVHFAEVLVDKETKDIKVVD
                     FVAVHDVGRVINPLNLEGQLEGGIHMGLGYALSEELAFDEQGRPKATDFKTYKLFRTV
                     DMPKCQVAFVNSYEKAGPFGGKSIGECAVVPVAPAVANAVYNATDVEIHSLPIKLK"
     gene            complement(16534..17004)
                     /locus_tag="PC700_001377"
     CDS             complement(16534..17004)
                     /locus_tag="PC700_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HDF2580692.1"
                     /translation="MEKIKLNINDYMYEIEVEKNETLLHVLREKLNLTGAKQGCNSGS
                     CGACKVLIDGVDMKSCKLLAKNCEGKVIYTIESFANGENLHPIQEAFIEVGAVQCGYC
                     TPGMIITTQALLNRNASPNEDEIKEAFKENLCRCTGYVKIIEAVKLSAARLGGR"
     gene            complement(17066..18460)
                     /locus_tag="PC700_001378"
     CDS             complement(17066..18460)
                     /locus_tag="PC700_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2580693.1"
                     /translation="MIDILIKNAIIVTVNKDRDVIFDGAIAIQGDRILDIGNTVDIEP
                     KYLDAKKVIDANGKVIFPGFINTHNHLFQVLLKGLGDDMALHEWLNTMMFPSAKFLTE
                     QDTYDAAMLGCMEGLKSGITTMVDYMHTHNRPGLTDGIVKAYKDLGIRGVVGRGCIDL
                     GIHKELIEDVETVEKDLRRVFEKYHNSENGRIKICVAPSSMWAISEEMGKMLWNIVKE
                     YDSYFTVHISETEFARTATKDIHGEIDIKLLEKWGIVGPEVVAVHCVCITDEDMEMLK
                     KYDIKVSHNVASNMYLASGVAPVPEMLKKGINVSLGLDGAASNNAQDMVELMKLTALQ
                     HKVNNCDPLAISAEKVLEMATIEGARTLRMEDEIGSLEIGKKADLVIFNSMLSPKSIP
                     LHNPVSTLVYSASMHNVESVLVDGNVILENGKVTTIESEEQVLLNAQLAAEELCKRAN
                     VTNRMEGHKWNSLY"
     gene            complement(18533..19894)
                     /locus_tag="PC700_001379"
     CDS             complement(18533..19894)
                     /locus_tag="PC700_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HDF2580694.1"
                     /translation="MKTRNTSKYDVDGIPPLKESIPLALQHLLAMIVGNMVPAILIAN
                     VVGLNQGQATMLIQGSMLAAGLATFLQLYPIPLFKGFKLGSRLPVMMGMSYVFLGACL
                     SVAAKDGLAALFGAQIAAGVIVFFVGFGVKKIRHIFTPIVSGTIIACMGLGLFATAIK
                     NLAGGEGTQTFGSPINFTVGVIVAFVIIMINKYGKGLVKNSSILIGILVGYAISLVLG
                     LVDFSAVQGAAIVSLPTPAAFGLEFRPELIVMFTIIYIIGIADMMGACTIATVGAMDR
                     EVTDEELASVVLGNSITSIISSLFAALPTGVFSQNTVIVSMNKVTSRFVIALGALVLL
                     LAGISPALGAIMTTIPSCVVGGATLVVFSSIAMSGFSIMSMDGFTEENNLIAGVSIAT
                     SMGLTTAPQVLDQFPETIGTVLGGSSIVSGAIIAILLQTLFKLKSRKSAENVTSNLEE
                     NIG"
     gene            complement(20192..21514)
                     /locus_tag="PC700_001380"
     CDS             complement(20192..21514)
                     /locus_tag="PC700_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDF2580695.1"
                     /translation="MFDIDGRPPILKCLPLSMQHILAMIVGTVTVPIVVGGAVGVSPT
                     QITLLVQYALIIAGISTLIQLYPIGNIGSRLPIIFGVGFTYVPTLVAVGGNYGLASIL
                     GAQLIGGVATVLIGIFIKKIRKFFPNIVAGTVVFTIGLSLYPIAINYMAGGVNSPTYA
                     SMQNWAVAAITLVVVIGLSQFAKGYAKLAAIFLGIIVGYCVSLVLGIINFDPIREATW
                     FAIPKPLEFGMEFDMPVIISVVIVSIINAVQAIGDLSATTMGGMDRNITDKELSSGVI
                     GSGICTMIGALFGGLPPSSYSQNVGMVAMNKVISRFVLGIAGIFMLLSGFVPKFGALM
                     TTIPYSVLGGATISIFSMITMTGIKLIITEELSSRNITIVGLAIALGMGITSVPAAQE
                     AFPSFVKLVFGESPIVIATLVAFVLNLIIPNKSLEDEAKERAVMEETN"
BASE COUNT         6439 a         4410 c         2397 g         8430 t
ORIGIN      
        1 agtaatatta tttttatagt aaaaatttta ttcatattac tatttataat ttttatagag
       61 caattttagt gccaatattt gcatacattt gtatagtata aaatttttat gttctcattg
      121 ataaaatttt acttatttca agcgttttat tcgattttat catctaaatt ttaggtttta
      181 tttttataaa ttaattgtat ttacataaaa aaaaagacct aaaaacaaat ttttaacggt
      241 cttttctcaa ttttttattt tcatttctaa aatttataat aaatagtaag aacactataa
      301 attcttttat ataaaattct tagtttgtgg atgataaatt agtgaattaa aatactactt
      361 tactgatttt agtttgacaa tatcactcca atttttacta gtggctaact ctactaaatt
      421 taagtctctt cttatttcag ctatatcctc ttttatagaa ttagtgtcac cttttatata
      481 tgctatgtta tgttctatat tatcatgagt agctttattt atttcagaat tatgtaataa
      541 acttcctaaa atattattct gttctatctg tatctctttc attgatttat cttcacattc
      601 tagactatca atcttaattt caattccata taatttttca tttgctatgg atacctcgtc
      661 cttaattcca ttcatatctt cattaatgtt atttatttta ttttgcataa agtttacttc
      721 tgatttaact tcggaaacat cttctttcat tacagttatt tcttgtctta cttctgacat
      781 gtcttttttc attacattta tctcttgttt tacctctgat gtatcttctt tcattacagt
      841 catttcttgt cttacttcag acatatcttc tttcattata tttatctctt gtcttacttc
      901 ggacatatct tcttttaata catttatctc ctgttttact tccgacatat cttctttcat
      961 tacagtcatt tcttgtctta cttcggatat atcctctttc attatattta tctcttgtct
     1021 tacctctgac atatcttctt tcattacagt catttcctgt tttacttccg acatatcttc
     1081 tttcattaca gtcatttcct gttttacttc cgacatatct tctttcatta caatcatttc
     1141 ctgttttact tccgacatat cttcttttaa tacatttatt tcctgtttta cttccgacat
     1201 atcttctttc attacagtca tttcttgttt tacttccgac atatctcctt ttaatacatt
     1261 tatctcctgt tttacttccg acatatcttc tttcattaca gtcatttctt gttttacttc
     1321 tgatatatct tctttcatta cagtcatttc ttgttttact tccgacatat cattttttat
     1381 gctaactaca tcttcattta tagtatataa taactctaaa atcttctctt gcacttgcat
     1441 caccatccct attaattatt ataacatttt acacaaatgc tatacaatgt taacatatat
     1501 atcagaatta ttaaaatttt aattaaacaa aattgattca ctttattact tcaaaactaa
     1561 ttaatatcta aaataactac aaataaacaa aaataaggtc aacaacaaaa aaggcttatc
     1621 tctttgagat agcctttctt atttttattt aaaactttgt tttttataaa ataatctata
     1681 cattcagctc tgaacatgaa atttttcttc caagtgcttt tggtttacaa actgatatac
     1741 aaagtccaca atatctacac tcttcttcat tcaattccat cttatcattc aattttctcg
     1801 cttgatatgc acaaactctc tcgcacatct tacatttagt acatttttct tcatcataag
     1861 taaactcaaa gctaagtctt tcattaacat ctcccatatg attatgtact ataccacaag
     1921 cattttctat agtattatat cctaatctat taagattaga ttttaaatca tcatgtattt
     1981 tagatattac ttgtgcgcct tttaaaatta ctactgaaca tataccaaca aagtcagcac
     2041 ctgccataat catttctaag gcatcatctc cacttacaca tccacctaag ccaattattg
     2101 gtatattaac ttgttttttt atttcatata ttctctgtaa agttattggc ttaatagttt
     2161 ctccacttaa atatcctatt ccatttccac ctaataaagg ttgccctgtt tctatgtcta
     2221 ttctaaatgc tggtcctaca gagtcacaag cagttattgc atctgctcct gcttcttcta
     2281 attttttagc aaaatcgcct atctcatcta tcataggagg aagtttaact attacaggta
     2341 tatttactct ttcctttgca tctttaagca ttggtatcaa atctctatag tcataagata
     2401 ctaattcaat aaagtcagct cctgcatttg caactttctc tactaattcg ctactttctt
     2461 caattgtatg acctactgaa gctatacaga cagtcccatc tcttttcatt tgtggtattt
     2521 caaaatctat ccattgctct ggctcaaaat ctgtccattt ttcattattt aaaagagcat
     2581 tagctgtatt tctaaccata tgtggtgctg gatttatagc tctttctttt cttatagtct
     2641 tagtcacaac tgcccctgct cctgcatcac ttaatatctt gcatccagct gcactatatg
     2701 ttaatggtcc tgaacctatt ataagtggtg attttaattc tttacctaaa aaattataca
     2761 ttatctgctt ccccttttca tatatattta cattcgttaa aatttccaaa attcacaagc
     2821 aacttctttc atttctttaa caatagcttt tttatctaaa tcaactaact gtttttcttt
     2881 taaaatatgt ctaccatcta tgtatacatt tgatataaat tcttttttac cctgaactac
     2941 tatctggtca aatatattat caagagtcac aggagtttta aattcatctt ccataactat
     3001 tatatcagcc ttattaccaa ctgctatttt accagaattt tgaagtccta atacataagc
     3061 accatgctct gttgccatcc taaatactaa atttgctggc attactgatg catcttcttc
     3121 aactgattta tgcattaaaa atgcacctct cataacttca aaaaaatcat ttatatatcc
     3181 atcagtaccc agtgccactt ttatgccttt tttaatcatt tttggtactg gagaaaaacc
     3241 tccacctact tcacagttgc taagaggcat atgaaccact tttacacctt tttcttttaa
     3301 tatatctatt tcttcatcat ttacttttac acactgagat gctaatacag tttcatccaa
     3361 tatatctaaa tcattatagt agtcaactgc ttttttgcca aaatgttttt cagcatagtt
     3421 tggctcatat atactctcac ataaatgaaa ttgcattggt gcatttaatt tttttgcatc
     3481 ttctttagct ttcttaataa atctatcaga acatgtgaag cttgtatgtg tacacattat
     3541 tccatttatt aatttactat tttctctact ccactttatt aaattagaat tttcatctaa
     3601 acatctaagt ccattttcaa agcttattct ttcacaactt tctaaggata aaatcgcttt
     3661 catgcctatt tcttctagta tttttccttg ctcaattaaa gttccttctt ctgtatttgg
     3721 agcttctagg gtatcacaaa aagctaccac accagattct ataagttcta ttgctgatgc
     3781 tttagttgtt gccttcactt ctttcaatcc aattctattt tctataaatg gccaccaata
     3841 gtcatttaaa aaaccttcaa agtcagtaaa atgtacattt gccggtattc ccctagaaag
     3901 aactccatat tgatgcatat gaccattgac gaatcctggc attattatag catctgattt
     3961 atctattatt tcttcaatat ttttatattt tatttcaact tcttcatttg gcaatatatc
     4021 ttttataatg tcattttcta ctacagcagc agaatttgta tatatttcat cagcagaaga
     4081 tattaagtat ttacttttaa tagcaatcat ttatttacct ccctgattta attttttcat
     4141 ggccatataa actttctcag aagttatagg aagttctctt atacgaactc ctgtggcatt
     4201 gtatattgca tttgctattg ctggtgcagg tgtatgaata actatttctc ctaaagattt
     4261 agctccatat ggacctgttg gctcataatt atctacaaaa tcaacaatta tgtttcctat
     4321 atcttttttt gttggaactt tgtattgtaa tagattagaa gtttgaagct ttccatcaga
     4381 accatgtcta gggtcttcat acattgcaag ccctatacct tgagttacac caccttcaac
     4441 ttgtattcta gcaagtgcag gattcataac tgtaccacaa tctgtagaac aagcataatt
     4501 tattacttta aactcccctg ttgttttatc taactctatt tcagcaaatc ctgctaagaa
     4561 tggtttagca ctctcttgta taccataaga ttcagatgaa tataaaactt cttgatttcc
     4621 accaccaaca gattcttttc ctaattcact taacaaaaca aacttctcta catcttcact
     4681 ataacaaact ctgtcttcat atagtattaa atcatctatt ggtaaatcga gtttcttctg
     4741 tgctactttt aatatctttt gttttaaact ttcagcagat tttattgtag catttccagt
     4801 tacataagta gtacaagaag cgtatgcacc agtatcatat ggacacatat ctgtatctcc
     4861 tgtataaacg gaaattctat caatagatgt attcaatgct tttgcagcta tttgtgcaag
     4921 tattgtgtca gaaccagttc ctaagtctga agacccagaa aacaacttat aagttccatc
     4981 ttcatccatc ctcatattta ctattgccat gtctatgttg gctataccag aaccatgagt
     5041 tcccacagac attccaacag ccctcacctt attgtttcca atatccctaa cagggtactt
     5101 ttcatcccat ccaataagtt ttttacctct aattatacat tcatcaagtg cacaacttct
     5161 tattggaaaa ttcattattc cgccttcagt ttgttttttt ataatattct ttaactttat
     5221 ttctgttggg tccatattaa gtttatgcgc taattcatca attgcacaat caagtgcaaa
     5281 tgtaccttgt gttgctccat atcctcttaa tgctcctcct ggaactaaat tagtatatac
     5341 agttctccca tcaaatttta ttgcaggtac attattatat gttggaagta cattatgacc
     5401 tgattcagag caaactgaag gtccattatc accataagcg cctgtattat tcaatgcttt
     5461 catatctata gcttttatgt ttccttcttt atctgcccca atttttatat caaaaaacat
     5521 ctgatgtcta gtattagtca ttgcaaaagt ttcttctcta gtataaatta gttttgctgg
     5581 tctttttgtc atccaagtta caaatgatgc atatatctct gttacagcta tgttttttcc
     5641 accaaaacct ccacctattc ttggttttat tactctaact tttgaaagag gtattcccag
     5701 tgctttagaa acctgtcttc tcatatgata agctgattgg tttgcagaag ttactactaa
     5761 tctgccattc gtatctatat acgtataagc tctatgagtt tccatcatac aatgggcttg
     5821 tgattgagtc atataagaat gttctactat tacatcactt tctttaaact ctttttctac
     5881 atccccttta cccatttttt ctctcgatac aatatttttt gtagcatcca atccaaaatc
     5941 aaatggacaa aacaaatcat cttcttcatg gattaaaatt ttatttcctt ccgattctct
     6001 tgcatccaat ataggagtca ttacttcata ttcaactttt ataagtttca tagctttaat
     6061 agcagccctt tcatcttcag cggctattat agcaacttca tctcctacat atctaactac
     6121 gtcttcaagt attaattggt catgaggtga tgcttctgga tatgactctc caaccattga
     6181 atatctataa tttggaacat ctttgtaagt gaatatgttt actacacctt ttacctttaa
     6241 tgcatttttt gtatctatgt tttttatctt cgcaaaagca tgcggacttc taagaagctt
     6301 aattgttaaa acatctttat gaaaaattaa atcttctgta tatattggtt ttccagtaac
     6361 aatggcttca ctatctattt ttcttacctc tttatttacc atcacactca cctccaatac
     6421 tattatacat tctcactact aaggcttttg ccatatcttt tctgtattct ttacttcctc
     6481 ttatattact tccaaatgaa agttcttcac ttgatacttc accagctttt tctatatcat
     6541 tttctaaact taatatttca cttgctttat aggcaatttt tgctcttctt ggtctagcgc
     6601 ctatcgcaat tttataaata cttccttctt ttaacattgc tccatttaat actgcaaaat
     6661 cacctgtaca ttttcttata gaatcaaata cacctatagc atctttttta ggtaatataa
     6721 cttctattaa tatatctttt tcaagctcac tttctaaaaa atcagctaag ttcataaccc
     6781 cttttttata taacttaacc tttgcatcta ccactagtaa tgaaggtatt aaatctgaaa
     6841 atccatactt agcaaatacg ctagctccta ctctggctcc acttctaaat tgaactccta
     6901 ctatattaga aacagcgttt gaaattaccc cattacagta atttttaata accttattta
     6961 attctaaagt tcttaaactt gtgtctgctc caattaatat gctatcatca gtttcattta
     7021 tataatctaa agctaagtct tttaaatcta ttgctgagcc aatattttta ctacctagtt
     7081 ttaaaaatcc acatcctcca agtattatgt tgttttttct tgatattagc agttcataag
     7141 cttcatctaa actttttgga actacatatt cctttatagt tatcatactc tctccccata
     7201 tcaacttaat tttcacttaa aagtctaagt gtttaaatat ttatgtattg ctcttaattg
     7261 acctttatat cctgtacatc tacaaaggtt accatttaca taatctataa cttcttcttc
     7321 tgttggattt tgaagctctc ttttcattgc caaaaccatc attataaatc caggagcaca
     7381 aaatccacac tggtcagccc cttcgtctgc aagaaaatct ccaaacttct ttgcttcatc
     7441 caagaccccc tcaagtgttg taatatgctg accatctact ctagcagtaa gataattaca
     7501 tgaaagcacg gccttgtcat ttaccatcac tgtacataac ccacatgagc ctgtatcaca
     7561 ccctttttta acacttacat atccattttc tcttaatgta tctgctaaaa aatcatctgg
     7621 tcttatactt aattcttttt ctttattatt tatggtacat ttgataatca aataaatcac
     7681 ctcaaaactc atatttttaa gctaaagttt tactatactt ttctatgaat aaattacatt
     7741 tattaataaa ggctattcca tttatgacct tccattctat tttttatagt tcctcttaca
     7801 caaagtctct cagctgtatc ttgagcatct tttaatgctt tctcttcatt agctgtcaat
     7861 atttttgaat cttccattat tatatttcca tctactataa cactctctat atttttcata
     7921 ctagaagaat atactaatgt agataccgga ttatgcatag gtatcgcttt tggtgaaagc
     7981 attggattaa atattagtaa gtctgctttt ttacctatct ccaatgaacc tatctcatct
     8041 tccattccaa tagctcttgc tccatctata gttgccaact caagaacttt ttctgccgac
     8101 attgctaatg ggtcacactt attaacttta tgttgaagtg ctgttaattt cataagttca
     8161 agcatatctt gagagttatt actagcggct ccatctactc ctaaactaac tgttattcct
     8221 tttttaagca tttctggcac tggtgcaaca cctgaagata agtacatatt acttgctgta
     8281 ttatgagaca ctttcatatc gtatttttta gtaagttcca tatctttttc agttagatac
     8341 acacaatgta ccataagcac atttggtcca agtattccta atttttctaa aacatctatg
     8401 tcatattgcc catgcaattc ttttgctgct tctctatcaa aaggagtctc tgatatatgt
     8461 actgtaaata aagcatcatc atattctttt accactctcc atagcatttc aagcatttct
     8521 tgagaatttg accatattgc tgcaggagct actcctattt tgattcttcc attttctgta
     8581 ttatggtgtt tttcaaacaa tcttcttaca tctttttcaa ccgtttctac atcttgcatt
     8641 attccaggat gaactccaaa ttgagctcct gtattcatac atcctcttcc aagtattcct
     8701 cttattccta attctttata agcatctatt attccatcac aaagacctgg cttactatga
     8761 ggatacatgt agtcaaccat ggtcgttata ccacttctaa gtccttcaat acatccaagc
     8821 attgcagcat cataagtatc ttttggctct aaatagttag ctgctggaaa tgtcatagtt
     8881 tctaaccagt ctttaagtac catatcatct cccaatcctt ttaataaagt ttggaataaa
     8941 tgattatgtg tatttataaa acctgggaat aaaactttac ctttagcatc tataattttt
     9001 ttaacatcag tatatttact ttctatctct ttagaatttc caatatcagc aatcttgtta
     9061 tcttttacta ctaatgcccc atcaaaaata acttctctct ctttattcac agttactatt
     9121 atggcattct taataagtat atccatcatt cttatttccc cccgcattat ttagtaattt
     9181 ttcttttaat atattgacct gttggatgtt ctttaacatt acaactttgt actacatgat
     9241 ttcctctcaa aaatacatct tcaactttac atttaacctt aaatccttca tatggagtat
     9301 aatctacatt ttgtttttga gttttataac taattgtttc ttctttattg atatttaaaa
     9361 tgactatatc tgcatcactt cctacagcta tctcaccttt tttaggatac attccaaata
     9421 tttttgcagc attcgtagaa gtaacttcca caaacttatt tacacttatt ctattttcta
     9481 agaccccata tgtatataaa agtgctaacc tatgttcaac accaggtgct ccatttggta
     9541 tcttactaaa atcatcaatt cccaagtctt tttgcccctt aaaattaaat gaacagtggt
     9601 ctgtgccaac tgtttgaata tctccactag acaatccttc ccaaaggtaa tttatgtctt
     9661 ctttttttct taaaggagga gacattacat acttagcacc ctcaaattta ttcttattat
     9721 ataaattgtc ttctaataat agatactgtg ggcatgtttc tactatcata tctatatttt
     9781 tttctctagc catttttaca gtttcaagtg actccctaca acttaagtgg actacatagg
     9841 attttgaatt agataattta gttatatcag caagtcttga aacagactct ttctcaacta
     9901 tgttaggtct tgtaagtgga tgatattttg gctctaaatt accattttgt atattttctt
     9961 ctatcaatac atctagtaaa tccccatttt cacaatgaaa tccaactata caacctaaat
    10021 cccttgcttt ctttaaaacc ctgtatattg ttccatcatc tactttcatg ccatcatatg
    10081 ccatatacat cttaaaagat actatcccat ccgaaataag tttttccata tgattaaatg
    10141 tatctttatt taaacatgta attgtcatat gaaaaccata atcacaataa caatttccac
    10201 ttgctttttt atgatatgct tctacacctt gcagtaggtt ttccccctca ttggcttctg
    10261 caaaatctag tattgtagta gttcctccag ctatagctgc tcttgttcct gtttcaaaat
    10321 tatctgcagt tgtaatagaa cctgcattca tatcaaaatg agtatgtgtg tctattccac
    10381 caggaattac aattttacct gtagcatcaa taaccttatc accatttatt tctaagttat
    10441 ttcctatttc aactatcttt tcatcttcta tccttaaatc acttatataa gtcttatcag
    10501 aggtaattat agttccacct cttagtattg ttcccatata tacctcctaa cttttaagct
    10561 ttaaagtatt ttctaagtat cgtaaaactt tatttaaaat tgtattttct aaaggtgcta
    10621 tattcagcac ctctagacta tatttaaata atttattgtg aactaaatat tattttccgt
    10681 atacagattt taatattcca tagtaacctt ctgttcctat aaataactgt tcttgctcta
    10741 tatactcatc tattgtgtga gcaagatttt ccttagaagg accaaatcct atagttttta
    10801 ttccagcttc acctgcatag tgacttccat tcgtacagaa tgagtattga gttatttctg
    10861 aatctattcc agcttctttt aatcccttat atgctgcttg aacaaattca tcttcttcgt
    10921 catataacca acctgggaag aatctttcac tttctatagt atttcctgta tagcaatcag
    10981 ctttttcaac cgcataagaa actttcgcat ttaattctgg gtcttctttc atcatttcat
    11041 ctaataaagc ttgtattgga gctaatacgc cttctcttgt ttctccaact aataatcttc
    11101 tatcaaaagt agctttgcaa taatctggca ctactgatgc ccctggatat ggagaagatt
    11161 ttatatctgt taaaactaat attccatcac ctaaaactgg atgagttgga ggtactaatt
    11221 gttgtatctt ttggattaca ttagccattt tataaactgc atttacacct ttttctggat
    11281 tagcagagtg agctggttta ccaaaagttt ctactactat ttctcctcta cctctctgac
    11341 caatctttaa gtttaactct gaagactcac ctattacaac atagtcaggt tgaacagcct
    11401 tacttatctc tctagccgaa acaccttcaa atatttcttc atgaacaaca cctgctacat
    11461 atagttctcc agcaaaatct cgattagtgt ctttagcaaa atacgctaca gcagacatca
    11521 ttgcagtata ttgacctttc atgtcagaag ttcctctacc atatatcttt ccatcaacta
    11581 tttttccttc aaaaggagga acactccatt tacttgcatc tggaactgga actgtatcta
    11641 tatgtccatc aaataatatt ttcttacctg gcttgttacc ttttatacat cctactatat
    11701 ttccatatct atctcttgac caactatcaa aacctaattt ttcaaaagac tcttctattg
    11761 cttttacaac accttcttca tttccagaag aactaggatt ttgaattaat ttttggcata
    11821 tttcagttac ttcttgtttt ctttgttcgt ttaacatttc attatccccc tagatttttt
    11881 ataatgtatg tattttatat acttatatat tttctaaatt ttagaactta tatatcttct
    11941 aaatatgtaa accaatatct aataattgca ttttagtaat tccttatata cttggatatt
    12001 ctccatccca aactatactt ctatatttgt cagggtcagt atctccttct gtgcttatta
    12061 aaagtattct tgaattttca tctaatttta aatcttttct taaatctgct aaatcttctc
    12121 tttccatagc tgctactaaa agaccagttg taactgctcc agattctcca gaaattacct
    12181 tttcatcacc atttaaagga ttacctaatg ttctcatacc tttagcagaa acccagtctg
    12241 gtgctgaaac aaatgctgca gcatgatttt ttaatacttc aaatcctata gtatttggtt
    12301 ctccacaagc aagaccagcc attatagttg gcatatctcc tccaacaaat cttggttttc
    12361 catctcctgc ttctgctgat ttatagtagc aatcagcttc atcagcttct actactactg
    12421 ttattgggca ttcatcgtat attgaagcaa catatccttg aactgctcca gcaagtgaac
    12481 caacccctgc ttgcacaaat acatgtgttg gtctatctac tccatattct tttaattgct
    12541 ctatcgcttc ttgagccata gttccatatc cttgcattat ccatgctgga atttcttcat
    12601 atccatccca tgctgtatct tgaaccatta ctccatgatc atcagcttct gcataatcat
    12661 tagctaatct tactgcatca tcatagttta agtctgttat actagcatct gctccttctc
    12721 ctttaatttt atttaatcta aactcagaag aaccttttgg cattaataca acagattttt
    12781 gacctaattt attagctgtc caagcaactc ctctaccgtg atttccatct gtagctgtaa
    12841 agaaagttat ttctcctaat ttttctttta tttctttcga agttaatttg tcatacccta
    12901 actcagatat atctgtatct aatctttgag ctaagtatct acccatagaa taagaaccac
    12961 ctaatacttt aaatgcattt aatccaaatc tataagattc atcttttaca tatactcctg
    13021 caacacctaa tttttttgct aaaccttcta ggtttgctaa tggagtttct ttatattgag
    13081 gaaagctttt gtgaaaattt ctaaccttag ttatttcttc ttcatttaaa aatttgatac
    13141 aattttcctt atcacccttt ggcaaattat ttactttcca tttaatttct tttaacatct
    13201 aaaatacctc caaattattg ttaaattttt atactttaat atataagcaa ttaacatgcc
    13261 aacactaaat atatactaga tatttttcta tgactcttta gtatagaaat tgcaatgctt
    13321 ttattattct ttcttttcta tctttagttc atacaatgta tgtagaattt aataactaaa
    13381 aaaccttttc tatcttctca ctttttaatt ctcattttta aaatttaaaa ataaatctca
    13441 aaacactttg tttctatatt ttttaatata aaatttataa aatctaaatt aaaacataaa
    13501 atagtgatgc aataatctat tacatcacta tttgtatata tttaataata taataaatct
    13561 ttagtggtct ctaagaggtg atttttcatc ttttttaaca tctaattctt cttttacgac
    13621 attccatttt tcaagaccaa acttagaaac cataactgcc tctgcagcac cttttccaag
    13681 tctttttact gtgtccatac ctgtctcatc ctcttgggca ccttttgctt ttccatcttt
    13741 actccatatt gtaccaccta tatagcatcc acctaaacct ccagatacta acatttcatg
    13801 cattaggaag aaatttatta taggaagttt tgtcaattct tggccaccat atctagttcc
    13861 accagtagtt atagctgcac caaccttatt ttgaagtttt cctggatgaa ctaaatacat
    13921 tggacgaagt ctattaaata ctgctgtaag ttgagcagtt atattcatat cataaactgg
    13981 agaacctact ataattccat ctgcctccaa tattttttgc tctaattctt ggatatcatc
    14041 ttttataata cacatagttt cttttcttat acatgcatca caatgtacac aaggacttat
    14101 ttttttccct ctaactgacc aatactcagt ttctatacca ggtatagttt ctgcttcttt
    14161 caaagcttct aacactgcat attctgttgc tcctttacgt ggacttccac aaatacctaa
    14221 tatctttgcc ataataatat ctcctcacca aataaatcta tttttattat ctatttacaa
    14281 atacaataaa ttgttgtttg tgctttaatt tctactattt taattttatt ggcaatgaat
    14341 gtatttctac atcagttgca ttatatactg catttgcaac agctggcgca actggtacaa
    14401 cagcacattc tccaatactc ttaccaccaa aaggtccagc tttttcataa ctatttacaa
    14461 atgcaacttg acattttggc atatctacag ttctaaataa tttatatgtt ttaaaatcag
    14521 tagctttagg tctaccttgt tcatcaaatg ccaattcttc actcaatgcg tatcctagac
    14581 ccatatgaat cccaccttct aattgacctt ctaaatttaa aggatttata actctaccaa
    14641 catcatgtac agctacaaaa tcaactactt ttatatcctt agtttcttta tctactaaaa
    14701 cttcagcaaa atgtacacca taagaagtaa gtccagctgg tgaagaataa tcttcaacaa
    14761 cagttaactc tcttaatgat ttactttgac tatatactgc gacatcagaa agtgatgctt
    14821 ttacattttc atcaagttta ctaattacat atccattgtc tagatataaa tcttcttttg
    14881 atgtttcaag taatttttct gcttcttcta ctataaggtt tttcaatttc tctgctgtct
    14941 ttttagctcc tccaccttct acaaataccc ctctacttgc atagtcacca aggttgaatg
    15001 tacatgcatc cgtatcagtt tcaaatgtat caactttatc aggtgttatt cctaaaactt
    15061 cagctaccat cattgtttgc atagttacga caccattacc catatcatgt gctcctgtta
    15121 gtagtgtcaa tgtaccatct tcatttaatt taagagtcaa tgtaacaaca tctctatgtg
    15181 caccaaaaac tccattacca tgtgcaccta ttgccatacc tatacctctt aaatattttc
    15241 cttcttcttt tggaagtttt ttcttttcat cccacttaaa catcttctta ccttgctcta
    15301 tacaatccaa tactctagga tttccaagag aaccgcggaa gattatatcg acatcatctg
    15361 gctctactgc atttttattt tgaaaatcga ctaaatccat acctatttcc ttggctattt
    15421 tcgcaaattg agcctgttga gccataaata tttgaggtga cccatagcct ctcatagctc
    15481 ctgctattgg tgtatttgta tatacaggca tcccagtaaa ctttatattt ggtattttat
    15541 aaactttaaa tactttatga ctaagtgctc ctataacatt taaagcactt gatgcatatg
    15601 ctcctgtatt tgtatatatt tttatatctt gtgctattac ttcaccatct ttatttacac
    15661 cagtttttaa ttttacaaaa gacccatgac gagttcttgt tgatatcata gattcctttc
    15721 ttgtaagaac cattttaact ggtcttcttg tcatttttga taatgcggct gctactggtt
    15781 caattgtaag ttctaatttt ccaccaaatg agcctccaat agcaggtctt ataactctta
    15841 ctttatttaa tgacatccca aaaatttgag atagtattat tctaaatcca aatgtatttt
    15901 ggcaaggtgt atgtacagtt aatttatcat tataatcata gtgtgcaatt acactatgag
    15961 tctctattgc actatgatgt attgctggag tagagtattc atcttcaaat atatagtcac
    16021 attccttgaa gcctttttct acatctcccc catttatgtt gttttgagaa actatatttg
    16081 aatctccatg tatgcagata gcatcttctt ttattgcatc ttgtatagta aatacagctg
    16141 gcaattcttc atattcaact tttataagtt ttacagcttt ctctgctatt tctggagtct
    16201 ctgcagctac tgctgcaaca cggtcaccaa caaatctaac tgtatctgaa aatataactt
    16261 cattttcagg tatattgtgc tcataaaatc ttaatgaacc attgtatttt ctttgtgatg
    16321 tattgagata agtagctata gctctaacac ctactacttt ctcagcttca cttgtatcta
    16381 tactttttat ttttgcatgt gctacaggac ttaaaagcat cttagcgtaa agtacatttt
    16441 gtggtttcat atcagccacg tatttcattt gaccagtaac ttttaaagct gcatctttta
    16501 gagggtatga ttttcctatt accttttcca taattatcta ccccctaatc tagctgcaga
    16561 taattttaca gcttctataa tctttacata tcctgtgcat ctacagagat tttctttaaa
    16621 agcttcttta atttcatctt cattaggaga tgcatttcta tttaaaagag cttgtgttgt
    16681 tataatcata ccaggagtac aatatccaca ttgtacagct ccaacttcta taaaagcttc
    16741 ttgtattgga tgaagatttt ctccattagc aaaactttct atagtgtata taactttacc
    16801 ttcgcagttt tttgcaagaa gtttacaaga tttcatgtca actccatcta tcaaaacttt
    16861 acatgctcca caactgccac tattacaacc ttgtttcgct ccagttagat ttaatttttc
    16921 acgaagtaca tgcaaaagag tttcattctt ttcaacttct atttcataca tgtaatcatt
    16981 aatatttaat tttatttttt ccattttctc ctcctacaga taaactaatg ttgtaataga
    17041 atattattac aatcttattt taatattagt ataagctatt ccacttatgt ccttccattc
    17101 tattagttac attagctctc ttacataatt cttctgctgc aagttgtgca ttaagcaaca
    17161 cttgttcttc actttcaatt gtagttactt ttccattttc aagtattaca tttccatcaa
    17221 ctaaaacact ctctacattg tgcatactag ctgagtatac caaagttgat acaggattgt
    17281 gaagaggtat tgattttgga gaaagcattg agttaaatat tactaggtca gcttttttcc
    17341 caatctccaa agaacctatt tcatcttcca ttctaagagt tcttgctccc tcaattgtag
    17401 ccatttcaag aactttctct gcagaaattg ccagagggtc acaattattt actttgtgtt
    17461 gtaaagctgt taatttcata agctctacca tatcttgtgc gttattactt gctgcaccgt
    17521 caagacctaa actcacattt ataccttttt ttaacatttc tggcacaggt gcaacaccag
    17581 aagcaagata catattactt gctacattat gagaaacttt tatgtcatac tttttaagca
    17641 tttccatgtc ttcatcagtt atacatacac aatgtactgc tacaacttct ggacctacta
    17701 taccccattt ttcaagtaat tttatatcta tttctccatg tatatccttt gttgctgttc
    17761 ttgcaaattc tgtttcagat atatgaactg taaaatatga atcatattct ttaactatat
    17821 tccaaagcat cttacccatt tcttcagata tagcccacat tgaagaagga gctacacaga
    17881 tttttattct tccattttct gaattatgat atttttcgaa tacccttctc aaatcttttt
    17941 caaccgtttc cacatcttct ataagctctt tgtgaatacc taaatctata caacctctac
    18001 ctacaacccc tcttatacct aaatctttat atgcctttac tattccatct gtaagtcctg
    18061 gtctattgtg agtatgcata taatcaacca ttgtagttat accacttttt aaaccttcca
    18121 tacatccaag cattgcagca tcataagtat cttgttctgt taagaatttt gctgatggaa
    18181 acatcattgt atttaaccac tcatgaagtg ccatatcatc acctaaacct tttaataaca
    18241 cttggaacag gtgattatga gtatttataa agccagggaa tattactttt ccattagcat
    18301 ctataacttt tttagcatca agatacttag gctcaatatc aactgtattt cctatatcca
    18361 agattctgtc accttgaata gctattgctc catcaaatat aacatctctg tctttattaa
    18421 ctgtaacaat tattgcattt tttattagta tatcaatcat cttaaatccc cctttgaata
    18481 ctttcattac aacattttac catatggttt tctcatatta tatctaatac atttatccaa
    18541 tattttcctc aagatttgaa gtaacgtttt cagcactttt acgtgacttt aatttaaata
    18601 atgtttgaag taatatagct attattgctc cagaaactat acttgaccct ccaaggacag
    18661 ttccaattgt ttctgggaat tggtctaaaa cttgtggagc agttgtaaga cccatacttg
    18721 ttgctataga tacacctgct ataagattgt tttcttcagt gaatccatcc atactcataa
    18781 tagagaaacc agacattgct attgatgaga aaactactaa agttgctcca ccaacaacac
    18841 aagatggtat agttgtcatt attgcaccca atgctggaga tatccctgct agtaataata
    18901 caagtgcccc tagtgctatt acaaatctac ttgtaacttt gttcatagat acaattactg
    18961 tattttggct aaatactcca gttggaagag ctgcaaatag tgatgaaatt atagaagtta
    19021 tagaatttcc taaaacaact gatgctaatt cttcgtcagt tacctctcta tccattgctc
    19081 ctactgtagc tattgtacag gcacccatca tatcagctat acctattatg taaataatag
    19141 taaacattac tattaactct ggtctaaatt ctaatccaaa agctgctggt gttggtaaag
    19201 atactatagc tgctccttga acggctgaga aatcaaccaa tcctaaaact aaagatatag
    19261 cataacctac taatattcct attaatattg aagaattttt tactaaacct ttaccgtatt
    19321 tatttatcat tattattaca aaagcaacta taactcctac tgtgaagttt attgggcttc
    19381 caaatgtttg tgtgccttct cctccagcta aattctttat agctgttgcg aatagtccaa
    19441 gtcccataca tgctattata gttccagaaa ctattggagt gaatatatgt ctaattttct
    19501 taactccaaa tcctacaaag aatacgatta ccccagcagc aatctgtgct ccaaatagag
    19561 ctgctaaacc atctttagct gctactgaaa gacaagctcc taaaaataca taactcattc
    19621 ccatcattac aggtaatctt gaacctaact taaatccttt aaataaaggt attggatata
    19681 actgtaaaaa cgttgctaaa cctgctgcta acatagaacc ctgaatcaac attgttgctt
    19741 gcccctggtt aagtcctacc acgtttgcta ttaatatcgc aggtaccata tttcctacta
    19801 tcattgctaa cagatgttgt aaggccaaag gtattgattc ctttagtggt ggtatcccat
    19861 cgacatcata ttttgaagta ttacgtgttt tcataaaata aatcctccca tacataaata
    19921 tcttgactaa atatttttat aattattttc tataaatatt tttatgacca tgaatttatt
    19981 gctcaaccga taaacacagc taaactgagc tatttttata aaaaattaac aaatttctaa
    20041 atattccctc cccgattttc aacttaaatg aaacattaat tgcctggtat atatttattt
    20101 gttaaatttt ttattaaaat gaatagctat caaaaataat ctaacaatct catactaaat
    20161 aatttaaaca attcttttaa attattatat cttaattagt ctcttccata acagctcttt
    20221 ctttagcctc atcctctaga cttttatttg gtattattag atttaaaacg aatgcaacca
    20281 atgttgcaat tactattgga gattctccaa atactaactt tacaaatgaa ggaaatgcct
    20341 cttgggctgc tggtactgat gttattccca tgcctaaggc aattgccaaa ccaacaatgg
    20401 taatatttct tgatgacaac tcttctgtaa taattaactt tatacctgtc attgttatca
    20461 tactaaatat agatattgta gctcctccta aaacagaata tggtatagta gtcattaatg
    20521 caccaaattt aggcacaaac cccgacaata acataaatat accagctatt ccaagcacaa
    20581 atctacttat tactttgttc atagctacca tccccacgtt ttggctatat gatgatggtg
    20641 gtaaacctcc aaacaaagct cctatcatag tacaaattcc acttcctatt actccacttg
    20701 ataattcctt atctgtgata ttcctatcca tgcctcccat tgtagtagct gataaatctc
    20761 caattgcttg aacagcattg attatactta caattactac tgagattatt acaggcatat
    20821 caaattccat cccaaattca agcggtttag gtatcgcaaa ccaagttgcc tctctaatag
    20881 ggtcaaaatt tattataccc agaactaaag atacacaata tccaactata attcccaaaa
    20941 atattgctgc caattttgca tatccttttg caaattgact aagacctata actaccacta
    21001 atgttatagc cgctactgcc cagttttgca tagaggcata agtaggacta tttacaccac
    21061 ctgccatata atttattgct ataggataca aagataatcc aattgtaaaa accactgttc
    21121 ctgcaactat attcggaaaa aattttctta tcttttttat gaatatccct attaatactg
    21181 tagccactcc ccctattaat tgtgcaccca aaatgcttgc taatccataa tttcctccga
    21241 ctgctacaag tgttggtaca taagtaaaac caactccaaa aattataggc aatcttgaac
    21301 caatatttcc tattggatat agttgtataa gtgttgatat accagctatt attaatgcat
    21361 attgcactaa aagtgttatt tgtgtagggc taacacccac agctcctcct acgactatag
    21421 gtactgtaac agttccaact atcattgcta gtatatgttg cattgatagt ggcaaacact
    21481 ttaaaattgg tggccttcca tcaatatcaa acatagaaac attcttttga tatttactca
    21541 aggttatcac tcccaactat tttttaatga cagttattat tatctcacac aacatccttt
    21601 ctttttttat tttgtcatct caatttaata tgaagcaatt ataatgccaa ttttttaaca
    21661 taattgtttt acatat
//
LOCUS       DAMJWP010000042        21225 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.043, whole genome shotgun sequence.
ACCESSION   DAMJWP010000042 DAMJWP010000000
VERSION     DAMJWP010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21225
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.043"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            379..2457
                     /locus_tag="PC700_001381"
     CDS             379..2457
                     /locus_tag="PC700_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDF2580696.1"
                     /translation="MIKISEDYIEKRFDNELLRIEAWGKNSLRIRSFVDQNFVDENYA
                     LNEKPKLNKDDITINKNEDGSAIIKNGKIKAVLDHRDRITFYNDKNEILLKEYIRLRA
                     VKHDDGGEDVGTIEITKDFNSTLKLKSREYRPSYSGEFEVTTRFESEPSEKIFGMGQY
                     QHKFLDLKNTVLELAQRNSQISVPFYISSLGYGLLWNNPGIGKVSFAKNMTEWKMFST
                     NFIDYWITCGESPKELNKNYSQVTGTVPMMPENLLGLWQSKLRYRTSEEVLDVVKEYS
                     KRGIKLSSIAIDYFHWPKQGEYKFDLDYWKNPKELVKKLKEEYSVEPIVSVWPTVQSD
                     AENYNDYLENGYLVNVNRGVRMTMQIQGNTVFVDMTNENAREYVWDRIDKNYKQLGID
                     YYWLDVAEPGYSVYDFDNYRYKKGNVLSCGNIYPIDYLKMIYDGLHNDIESVVTLVRG
                     AWAGAQKYGALVWSGDIDSSFEAFNNQVNTGLNMGLAGIPWWTTDIGGFHGGNPKDPE
                     FRELMVRWFQYATFSPILRMHGDRLPHSKPLSNKGGGSMVTGAPNEIWSYGEEVEVIL
                     TKFIKIRESLKTYLKKLMKEAHEDGTPVMRTLFYEFPEDDKTWEVDNTYMLGDEILVA
                     PIMNYKDRSRKVYLPKGHTWENIFSGVSYEGGKTYEVECPLEEIPIFLKQDSSYNFKE
                     LKNILGEVKS"
     gene            2457..3236
                     /locus_tag="PC700_001382"
     CDS             2457..3236
                     /locus_tag="PC700_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2580697.1"
                     /translation="MEPQLWQLLLIVLYGFFINYEKNSTMFGTYQPVTAGFITGLILG
                     DINTGLYIGGTLQLLSLGISNFGGASIPDYQTASIVATFITITTKQEASVGISIGIPV
                     ALLMVQLDVLRNTIGIWLVHKAEDGAKKGNYKNITYMQMLGVLLTAATTGIPVALSVI
                     FGPSLINTILKYTPEWLTGGLTVAGGLLPAVGIGLLLRYLPAKEYFSYLVIGFVLAVY
                     MKVPLLGVALIGGAIALIIYKKNLENQEQQYTVVGGMDEDE"
     gene            3229..4038
                     /locus_tag="PC700_001383"
     CDS             3229..4038
                     /locus_tag="PC700_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF2580698.1"
                     /translation="MSDKVSLNKSNEKVKKSVLWRWFFTSSVSSNYEKMQALAYCYAV
                     LPFLKVVYKDNPEKLQSAVLNHLQFFNTNPWVAPYILGINVAMEELSSEETEEAVASI
                     KTGLMGPVAGLGDSLFVVIPWTIFGAIAANMAIDGSPFGILLWIVVSVALKLLSIPLF
                     NAGYTSGTKLISSIEKSLKILTESTSILGLMVVGALIPSVVKANVALNFQQGDFTMKG
                     QEILDQIMPGLVPAILVVIVYWALKKNIKPIYLILGVMVVSIILYALGILK"
     gene            4058..4540
                     /locus_tag="PC700_001384"
     CDS             4058..4540
                     /locus_tag="PC700_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2580699.1"
                     /translation="MEMKGIKNIRIDDRLIHGQVATMWSNKLGVTRLMVVNDAVANNS
                     VQKQVLRMATPAGIASSIITEETAIKNITAGKYEGQNVLLIVKSPVDLIPFVEAGFKI
                     HNINVGNMSSRKDTTVLRPNISVTEEEKEAFKRLLDEGIEITTIMTPDDKKTYLVDIL
                     "
     gene            4561..4965
                     /locus_tag="PC700_001385"
     CDS             4561..4965
                     /locus_tag="PC700_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2580700.1"
                     /translation="MNSIILMSHGNMAEEVLNSAKMIIGDSIDYPTVNMKQDDGIEGT
                     IEKLKKALEVYKESSEIIIMADLLGGTPCNAALLVANDDSRIKIISGLNLGMVIESAF
                     LDTKELAAKLEKIGKDNISMVEQNASLLSDDE"
     gene            complement(5121..6281)
                     /locus_tag="PC700_001386"
     CDS             complement(5121..6281)
                     /locus_tag="PC700_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HDF2580701.1"
                     /translation="MVTDKYTIREMNERLVLEQIIKNGPISRASIASTIGLNKATISA
                     ITKKLIDESLVHEIGIGNSTHSGGRKPILLVFNKCAGISLSMDIGYDYIFSSLSYLDG
                     TIINSKKLTDIQVSKDNVIQLIDEIINSYNISKIDTPYKVIGLTLAIHGITCENKVLF
                     TPYYNLNEIDLYSILSKKYDFPIHIENEANLTALAENTFSTVHNSLLSLSIHSGFGSG
                     IIINNKLYSGRNGMSGEIGHTIIMPNGKLCPCGNRGCLEQYCSEKKVFEQLSSLENIP
                     KIDSDIVKQLYYEDNQNAKKVIHEFCSYLTIAINNAITTYAPEIIYLNSQIISDIPEI
                     LQITKDMLVSSFNKGINIEISSLGSEASLYGGSAVNIKSFLNIQNLTLINEI"
     gene            6489..8039
                     /gene="xylB"
                     /locus_tag="PC700_001387"
     CDS             6489..8039
                     /gene="xylB"
                     /locus_tag="PC700_001387"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HDF2580702.1"
                     /translation="MKLEYVLGVDIGTSGTKTVLFDKLGNIIKSCTYEYPLIQEKSGW
                     AEQDANDWWKAVVESIREVVQSSNISSECIKGIGLSGQMHGLVMLDNEGKTLRNSIIW
                     CDQRTVKECEEITDLVGEERLIEITANPALTGFTASKILWVRNNEPDIYINTNKILLP
                     KDYIRYKLTGEYATEVSDASGMQLLDIRKRDWSDEVLEKLNIDKNLLGKVYESQEITG
                     YVTRDVASLTGLKEGTIVVGGAGDQAAGAIGNGIVKDGVVSSTIGTSGVVFAYTKEPK
                     IDKEGRVHTFCHAIPNTWHVMGVTQGAGLSLKWFKDNFCQSEVEVSNSLGEDVYEIIN
                     NQVSQVPTGCNGLLYLPYMMGERTPHLDPYARGVFFGLSPIHSKKEIARSIMEGVSYS
                     LKDCMDIIENLNIDVNEVRASGGGGKSKVWRQMQADMFNQDVYTINSSEGPALGVAIL
                     ALVGAGIYENIQKACDAIIKTSTKLEPISENVDTYKIYHRLYKRIYKSLKDDFKLLDE
                     VVNTKINN"
     gene            8135..9472
                     /gene="xylA"
                     /locus_tag="PC700_001388"
     CDS             8135..9472
                     /gene="xylA"
                     /locus_tag="PC700_001388"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HDF2580703.1"
                     /translation="MSEIFKGIGQIKFEGVKSDNELAFRYYNPEQVVGNKTMKEHLRF
                     AMSYWHTLCGEGNDPFGVGTVERPWNNVTDPIEIAKIKVDAGFEFMSKMGIEYFCFHD
                     RDIAPEGRDLEETNKILDEIVEYIKVNMEKTGIKLLWGTANMFGNPRFVHGASTTCNA
                     DVYAYAAAQVKKAMEITKYLGGENFVFWGGREGYETLLNTNTELEMDNFARFLQMAVD
                     YAKEIGFTGQFLIEPKPKEPTKHQYDFDTATVLGFLRKYNLDKYFKMNIEANHATLAG
                     HTFQHELNIARINNVLGSIDANQGDLLLGWDTDQFPTNIYDATLAMYEVLKQGGIAPG
                     GFNFDSKVRRASFEVEDLFLAYIAGMDTFAKGLLIAHKLLEDEVFENFTKERYASFSE
                     GIGKDIVEGKVGFKELESYALQMPVIKNKSGRQEMLEAILNRYIYEVDTISNK"
     gene            9994..11193
                     /locus_tag="PC700_001389"
     CDS             9994..11193
                     /locus_tag="PC700_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HDF2580704.1"
                     /translation="MKKKVLFLVIILAFIILLGNSTYSQKNQLSEEEKVDDFNYVYNV
                     VKTGYPYLGVNKRLNNIDWLANKDEYTKRIKNTENDEEFIEELSSILSDLNNKHTEVI
                     DNKKRYELFKKSYSNNDWYDFLNDKKVVDRYDSMNLKIKMPDDIFVKKELILKDVIKG
                     QIGYMYLPSMSSKNGSINNDLKIIGDYINTLEKHKALIIDIRGNLGGSDRYWQGIVSK
                     LIKNDVKINGYRIYRNNNEIVKKYTNARNIKLNPVENLPASVKENAPKEIIKDFNDFE
                     DTSYTIKSTDDSKFKGNIYLLVDRKIYSSSESFAMFCKETKFATLVGETTGGDGGCID
                     PVLFNLKNSGLIVRMASCMYLNKNGICDEEFKTIPEFKVKDCKRTADFKDDNCIRKAL
                     ELENINY"
     gene            11360..12145
                     /locus_tag="PC700_001390"
     CDS             11360..12145
                     /locus_tag="PC700_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2580705.1"
                     /translation="MRLEEMIFLNYNKLNDTDLLIWKYITANKKHCSKMSINELAMKC
                     NVSRATITRFVKKLDLKGFSEFKVLLSWESEKVHVIENQSFDIACNSIIKYVEDQKNK
                     DYDALCKLLYESRTIYAYGSGDIQNVVAKQIKRMFLSCQEIIYDFEGRTFDDSFYNIV
                     SKNDVMIIISLSGENENTLNIARRLKLIGTKIISITEFKNNTLTDLSDESIYISATNI
                     SFLQMHPSYKLTMLYFILIELLFIKYSIYKRHRMITEGIDCKL"
     gene            12314..13879
                     /locus_tag="PC700_001391"
     CDS             12314..13879
                     /locus_tag="PC700_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003233606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2580706.1"
                     /translation="MMKKIQRFGGAMFTPTLLFAFAGIMVGFSIVFQNQSIMGSLATP
                     ENIWYQFWGVISSGAWMVFNQLPLLFAISLPIALAKKQQARACMEALATYLTFNYFVG
                     SMLSFWGKSFGVDFAAEISAGSGLVSIAGIKTLDTGMVGALLISGIVIYIHNRFYDKE
                     LPDFIGLFRGSSLVVAICFFVMIPVALLTCFIWPHIQNVIRHLQTFFINSGNIGVWCY
                     AFLQKILIPTGLHHFVYAPICYDSVVVPGGTSVYWATHIQDFQTSAKTLKEMYPIGFS
                     LSGLSKVFGSLGVFGAFYVTAKPEKKKKVLGLMIPATLTAVLTGITEPLEFTFLFVAP
                     LLFLVHAFLDACLQTISFALGVVGDFGGGIINWVVLNWLPLGMYHWKVYVVQVVVGII
                     FSFIWFFLFTFLIKKFDMKTPGREEDNEETKLYTKNEYLETKDEKGNKLSKASQQASE
                     YIKLVGGAENVVDVTNCATRLRLTLKDDSIISKEEDFKVVGAHGLVHNGKAIQIIIGL
                     SVPSVREEFENLL"
     gene            13893..15224
                     /locus_tag="PC700_001392"
     CDS             13893..15224
                     /locus_tag="PC700_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDF2580707.1"
                     /translation="MERKRQRILIAGGGSTYTAGIVTMLIESVAKFPIESIKLYDNND
                     ERQRKVAEACAIIVREKNPEIKFSYTTNPEEAFTDIDFVMAQIRVGLYALREQDEKIP
                     LKYGVVGQETCGAGGIAYGLRTIGPIIEMIDYMEKYSPNAWMLNYSNPAAIVAEACRV
                     LRPNSRIINICDMPVCLEEIFARILGLNSRKDFDVRYYGLNHFGWWTSIKDKEGNDLM
                     PKLQEYCAKKGYEEFTPQGQHKESSWLETMRAAKDLLEIEPTTLPNTYLKYYLMADET
                     VEHANPNYTRANEIMDRREKDTFEECERIIKNGTARDTWFDASEHSQFIVELACALAF
                     NTQERFLLIVPNNGAIENFADDAMIEIPCLVGKDMVEPMSIGKIPTFQKGLMEQQVAS
                     EKLAVEAWIEKSYQKLWQSFTMSKTVPSAKVAKEILDEMIVANKDFWPTFK"
     gene            15238..15726
                     /locus_tag="PC700_001393"
     CDS             15238..15726
                     /locus_tag="PC700_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580708.1"
                     /translation="MITNKIKNITAVLKDENIFLSVVMVCFLILPVLYIVYLELTSLF
                     TNISFSQLLNNDVNASLNLISNFSAVYSAYVIYNIKKNKEIKENMIAFYLVLLGQIFQ
                     IYTITIVIMFTYIYKFVGIKQIISSIKELQWGKSYKALFSSIAVCLISLFVMIIRIKL
                     IM"
     gene            15740..16207
                     /locus_tag="PC700_001394"
     CDS             15740..16207
                     /locus_tag="PC700_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2580709.1"
                     /translation="MFFKRKKEASTINSYIDGKIINLGEIPDKVFSTKVMGDGFAMIA
                     KDNILKSPTKGVISFIAQTNHAIGIKLQNDVQILIHIGLDSATTKKENFKLEVKQGQE
                     VNMGEKLVEIDNKLLENTLYIPLVIVENPNTVKYHLSNLNQEGNSGIKVINFD"
     gene            16210..17364
                     /locus_tag="PC700_001395"
     CDS             16210..17364
                     /locus_tag="PC700_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2580710.1"
                     /translation="MINKAIKFKFIIFYFFSYIIFAFASTKFTPFLSKLGYSAFERGI
                     ILSSYAITNILFQLLFGVLADKYQTMKKIVLISLSVYGIASVIMFSTESATFIIYLLL
                     VALSGGLLNTCCSLYDTWIIGCGDNINNYLSFIKTFGSIGWAIGSMVASYLILRFSYF
                     GLSVAIGLIVVLLIINILLLPDIEKINKKINVTNKDIIELLKDKKYILLVCILFLLYS
                     MVVANNCTVIDKMIALNATNSQISLKWSLQSLLEIPTYLAGSYLLKKYSSLNLLKLSA
                     IMITVQFLLFALTNDSNIIIILCVFQVFSTPLILITSKRLIFEVSPKKLRSSSQLIAL
                     SIFMGGSSLIIPTVAGFLSINIGYNYTLMILSLFGCLAYGLIVLLNYLQE"
     gene            17682..18434
                     /locus_tag="PC700_001396"
     CDS             17682..18434
                     /locus_tag="PC700_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2580711.1"
                     /translation="MKEILKIKNISKDYGIKGFKTNVLKNISLTVNEGDFIAIMGPSG
                     AGKTTLLNLMSTLDKQTSGEIILDGINISKVKNNELSKLRREKIGFIFQDYNLLDNMK
                     LMDNIALPLALGKKKSKEIEAKVFSIAKKFGLENHLDKYPYQLSGGQKQRGAAARSLI
                     TNPAVIFADEPTGALDSKSAYELLESLEKINRENNATIIMITHDPLTASYSNEVYMIN
                     DGNIKCKLNKGNSRKEFYGKIMDMLASMGGEM"
     gene            18435..20342
                     /locus_tag="PC700_001397"
     CDS             18435..20342
                     /locus_tag="PC700_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2580712.1"
                     /translation="MSILGIAYNNFKSNIRTYLAFFISMVFSVVVLTNFELLRYGDAI
                     NVLQEENKKFTLSVLVAVIMILSVFLFFFIWYATNIFFKNRSKEIGILSFMGLDLYTI
                     GKIYFVENMLIGISSCITGIIIGIITSRFFQVVIIKLSGFNIKVSNGLSIEAILYATL
                     IFISIFIIMAVKGFITICRSSVINLINISKKQEKIPKVGIGIYILAIVSLIVIGYGYY
                     LSTNIRTGDITSVLPVIGIIIVGTYGLFKSVMPVIFDIIIKNKKILFNGNNIIAINNI
                     NYRLNKNYKTYAIIAIIITTTISTLGASVAMKHIHKNAQEQRNVYTISIVSMDKDEVN
                     KKQIENIIKDTNKIKYSVNPELTILKKTDIKEAYDTTDTIMKYSDFCNTLKVNGNDKE
                     LKEYGERLVSGNNVIELKSPQTIASIATKEVKTKLDGQLYNISRGEVKVPVLGTGLNQ
                     SIIVVNDDTYFKLRDKGQQVYFYGAKVFQEENSQVMFNNIEKNLSLKNGYIQNGYKAQ
                     GESQWMKFAYVVLVFLFLVFAIVAGSIIYMKIYSDAYEDKEKYKILLKIGTTEKDINR
                     AILKEVAIFYTLPMLSATISSYFALRLAGDLLMNDLSGIYILSLVICLIIFVIYGAVS
                     VNKFKSVVYKN"
     gene            20494..21168
                     /locus_tag="PC700_001398"
     CDS             20494..21168
                     /locus_tag="PC700_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2580713.1"
                     /translation="MYRILLVEDDIDLSKEIALALEKWGFKVELIDDFEVVLDEFMDK
                     KPDVVLLDVNLPLYNGFYWCEKIRAISNVPLIFLSSRDSDMDLIMGINNGADDYITKP
                     FSIDILVTKINGIIRRVYNYSDSNSILYCEDLMFDVGKGIIKHKYKDKSIELTKNEIK
                     ILTLLLKNKNRVVSRESLMMTLWDNDEFVTDNALTVNMNRLRSKVKELGFDDFIKTKK
                     GIGYII"
BASE COUNT         8149 a         2257 c         3757 g         7062 t
ORIGIN      
        1 acaatatcta atatattatt atctgtattt ataaggtcac tatacatatt ttcaagtcca
       61 atattatcta cataataggc aaattttatt cctgtatgtg tttaaaaaaa actagatgta
      121 tataggtatc atcacagata ttaaaagtat aaataaaaga tattaagaac gactttcaag
      181 caacatatta gaaaatttta ctaaaatccg gaaagcgatt gacaaacaaa gtcaaataaa
      241 gtatattatt ggtaagttag tttaatgtac aaatgaatta tatataaaac ctaatttata
      301 aataacgata ctaaacgaca ttatttaact taagctacac aaagatagaa tattatacca
      361 ttgatttagg aggtagccgt gataaaaata tctgaagact atattgaaaa aagatttgac
      421 aatgaactat tgcgaataga agcatgggga aaaaactctt tacgtattag gtcgtttgta
      481 gaccaaaatt ttgttgatga aaactatgct cttaacgaaa aacctaagtt aaataaagat
      541 gatattacaa ttaataaaaa tgaagatgga agtgcaatca ttaaaaatgg aaagataaaa
      601 gctgtcttag accatagaga tagaattact ttttataatg ataaaaatga aatattattg
      661 aaagaatata ttcgattaag agctgtaaaa catgatgatg gtggagaaga tgtagggaca
      721 attgagatta ccaaagactt taactctact ctaaagttaa aatcaagaga atatcgacca
      781 agctatagtg gagaatttga agtaactact agatttgaat ctgagccttc tgaaaagatt
      841 tttggaatgg gacaatatca acataaattt ttagatttaa agaatactgt tttggagtta
      901 gcccaaagaa attctcaaat atcagttcct ttttatattt ctagtctagg ttatggactt
      961 ttatggaata atccgggaat tggaaaagta tcttttgcta aaaatatgac agaatggaag
     1021 atgttttcta caaattttat agattattgg attacttgtg gagaaagccc aaaagagtta
     1081 aataaaaatt attctcaagt tactggaact gttcctatga tgcctgaaaa ccttttagga
     1141 ctatggcaaa gtaaactaag atatagaact tcagaagaag ttttagatgt tgtgaaggaa
     1201 tattctaaaa gaggaattaa gctttcatca attgcaattg actattttca ttggcctaag
     1261 caaggtgaat ataaatttga cttagattat tggaaaaatc ctaaagaatt agttaaaaaa
     1321 ttgaaagaag agtattcagt agaaccaata gtatctgtat ggcctacagt tcaaagtgat
     1381 gcagaaaact acaatgatta tttagaaaat ggctatttag tcaatgttaa tcgtggtgtt
     1441 agaatgacaa tgcaaattca aggaaataca gtctttgtag atatgacaaa tgaaaatgca
     1501 agagaatatg tttgggatag aatagataaa aattataaac aacttgggat tgactattat
     1561 tggttagatg tagctgaacc tggctattct gtgtatgatt ttgataacta tagatataaa
     1621 aaaggaaatg ttttaagttg tggtaatatt tatcctattg attatttaaa aatgatttat
     1681 gatggtttac ataatgacat agagtctgtt gtaactcttg taagaggagc ttgggcagga
     1741 gctcaaaagt atggagcttt agtatggtct ggtgacattg attctagttt tgaggctttt
     1801 aataatcaag taaatacagg gttaaatatg ggactggcag gtataccatg gtggactact
     1861 gatattggag gtttccatgg aggaaatcct aaagacccag aatttagaga acttatggta
     1921 agatggttcc aatatgctac tttttcacca attttgagaa tgcatggaga tagattgcct
     1981 catagtaaac cattatcaaa taaaggtgga ggctcaatgg taactggagc tcctaatgaa
     2041 atatggtctt atggtgaaga agttgaagta attcttacaa aattcataaa aattagagag
     2101 agcttaaaaa cttatttaaa aaaactgatg aaagaagcgc atgaagatgg aacaccagtt
     2161 atgagaactt tattttatga atttcctgaa gatgataaaa cttgggaggt ggataataca
     2221 tatatgcttg gagatgaaat tttggtagct ccaataatga attataaaga tagaagtaga
     2281 aaagtatatt taccaaaagg acatacatgg gaaaatatat ttagtggagt tagttatgaa
     2341 ggtgggaaaa cttatgaagt tgaatgcccg ctggaagaaa ttcctatttt cctaaagcaa
     2401 gattccagtt ataattttaa agaactaaaa aatattttgg gagaggtgaa atcataatgg
     2461 agccacaatt atggcaatta ttattaatcg tactatatgg attttttata aactatgaaa
     2521 aaaattcaac aatgtttgga acatatcaac cagtaacagc aggttttatt actggtctta
     2581 tcttaggaga tataaataca gggttatata ttggaggtac attacagcta ttatcattag
     2641 gtattagtaa ctttggtgga gcatcaatac ctgattatca aacagcaagt atagtagcca
     2701 catttataac tattacgact aagcaagaag catctgtagg aatctctata ggtattccag
     2761 ttgctttatt gatggtacaa ttagatgttc ttagaaatac tattggtata tggctagttc
     2821 ataaagcaga agatggagct aaaaaaggaa attataaaaa tataacctat atgcaaatgt
     2881 taggagtact attaacagca gctacaacag gtattccagt tgctctatct gtaatatttg
     2941 gaccttcttt aattaatacc attttgaaat atacacctga atggttaaca ggtggtttaa
     3001 cagtagctgg aggactactt ccagcagtag ggataggatt attacttaga tatcttcctg
     3061 ctaaggagta ttttagttac ttagtaattg gttttgtttt agcagtatat atgaaagtac
     3121 cattattagg tgtagcttta attggtggag caattgcact tataatttac aaaaagaatt
     3181 tggaaaatca agaacaacaa tatactgtag taggaggtat ggacgaagat gagtgataag
     3241 gtatcattaa ataaatcaaa tgaaaaggtg aaaaaaagtg tcttatggcg ctggtttttt
     3301 actagttctg tttcttcaaa ttatgagaag atgcaggcct tggcatactg ttatgcagta
     3361 ctaccttttt taaaagtagt atacaaagat aatccagaaa agttacaaag tgctgtatta
     3421 aatcatttac aattttttaa cactaatcct tgggttgccc cttatatttt gggtattaat
     3481 gtagcaatgg aggagttatc aagtgaagag actgaagagg ctgttgcttc aattaaaaca
     3541 ggcttgatgg gaccagtagc aggtttagga gatagtttat ttgtcgttat tccgtggaca
     3601 atttttggag ctatagcagc taatatggcg atagatggaa gtccatttgg aatcttactg
     3661 tggattgtag tgagtgtagc attgaaatta ttaagtatac cactatttaa tgctggctat
     3721 acatcaggta ctaagctaat atcttcaatt gaaaaaagtt taaagatttt aactgagagt
     3781 acatctattt taggtttaat ggttgtaggt gcattaatac cttcagttgt aaaagctaat
     3841 gttgctttga actttcaaca aggtgatttt acaatgaaag gtcaagaaat attagaccaa
     3901 attatgcctg ggttagttcc tgcaatttta gttgtgattg tttattgggc tcttaaaaag
     3961 aatataaaac ctatatactt aattttagga gtaatggttg tttcaataat tttatatgca
     4021 ttaggtattt taaaataagg aaaggatgat attagatatg gaaatgaaag gtattaaaaa
     4081 tattcgtata gatgaccgtt taatccatgg tcaagttgca actatgtgga gtaataaatt
     4141 gggtgttaca agattaatgg tagtaaatga tgcagtagct aacaattctg ttcaaaaaca
     4201 agttttaaga atggctacac ctgcaggtat agcgtcatct ataattacag aagagactgc
     4261 tataaaaaat attactgcag gaaagtatga aggacaaaat gtattattaa ttgttaagtc
     4321 tcctgtggac cttattccat ttgtagaggc aggatttaaa atacataata taaatgtagg
     4381 aaatatgtca agtagaaaag atacaacagt tctaagacct aatatcagtg ttactgaaga
     4441 agagaaagaa gcatttaaaa gacttttaga tgaaggaatt gaaattacaa ctataatgac
     4501 gcctgatgat aagaaaactt atcttgtaga catcttatag atttaaagag gtgtattaaa
     4561 gtgaatagta ttattttgat gagtcatggc aatatggctg aagaagtctt aaattctgca
     4621 aaaatgataa taggggattc tatagattat cctactgtaa atatgaaaca agatgatgga
     4681 atagaaggaa caatagaaaa attaaaaaaa gctttagaag tgtacaagga aagttcagaa
     4741 ataattataa tggcagattt actaggtgga actccatgca atgctgcttt attagtagcc
     4801 aatgatgaca gtcgaattaa aattatttca gggctaaatt taggtatggt tattgaatca
     4861 gcttttttag atacaaagga attggcagca aaattagaga aaataggtaa agataatatt
     4921 tctatggttg aacaaaatgc aagtttatta tctgatgatg agtaggaaga ttatattaat
     4981 atttaaaaca aaatacttgt gttaatactt aattgatata caatttttaa aaaatatagc
     5041 taatcttgca taaactatat aataaatcta attttagaat gtataattta aagggttatg
     5101 gctgtatata gtcataaccc ttatatttca ttaattaaag ttaaattttg tatattcaaa
     5161 aagctcttga tatttacggc agagccacca tatagagatg cctcagaccc taatgatgaa
     5221 atttctatat ttattccttt gttaaaagag ctaactaaca tatcttttgt aatctgtaat
     5281 atctcaggga tatcactaat tatttggcta tttaaatata ttatttcagg agcataagtt
     5341 gtaatagcat tatttatagc tattgttaag taactacaaa actcatgaat aacttttttg
     5401 gcattctggt tatcttcata atatagttgc tttactatat cagaatctat tttaggaata
     5461 ttttctaaag aagaaagctg ttcaaatacc tttttttctg aacaatactg ttctaaacag
     5521 ccacgatttc cacaaggaca tagtttacca ttaggcatga tgatagtgtg accaatttct
     5581 ccactcatac catttctgcc gctatataat ttattattta ttataatgcc tgaaccaaat
     5641 ccactgtgaa tactgagact aagtagtgag ttatgtacag ttgaaaaagt attctcagct
     5701 aaagctgtta agtttgcttc attttctata tgaataggaa agtcatactt tttgctaagg
     5761 atgctatata agtcaatctc attaagatta taatatggag taaacaatac tttattttca
     5821 caagtaatcc catgtatagc caaagtaaga ccaattacct tataaggagt gtctattttg
     5881 gatatgttat aactatttat aatttcatct atcaattgta taacattgtc tttacttact
     5941 tgtatatctg ttaatttctt agaatttata atagttccat ctaagtaaga tagagaagaa
     6001 aatatatagt cgtatccaat gtccatactt aaagaaatcc ctgcacattt attaaatact
     6061 aataatatgg gttttcttcc gccactatga gtactatttc caattcctat ttcatgaact
     6121 agagattcat caataagttt tttggtaata gcggatatag ttgctttatt caatcctata
     6181 gtagaagcta tacttgccct agaaatagga ccatttttta taatttgttc aagtaccaat
     6241 ctttcattca tttctcgaat agtgtattta tcagtaacca atttgatatt cctccttaaa
     6301 ataatattgt aatacttttt acacaaaaat aaaaggttat tttgcattga caaagataat
     6361 taaatatttt attattagtt cataagttag tttaatatac taacaaaaat aaagcaagta
     6421 aaatatacct aaaatataaa aaaattagga taggaaaacg atagttatga agtggcattc
     6481 aaggagggat gaagttggag tatgtattag gtgtggatat tggtacatca ggtactaaaa
     6541 cagttttatt tgataaacta ggaaatatta taaaaagttg tacatatgaa tatcctctta
     6601 tacaagagaa atcaggttgg gcagagcagg atgcaaatga ttggtggaaa gctgtagtcg
     6661 aatctataag agaagtagtt caaagttcaa atataagcag tgaatgtata aaaggaattg
     6721 gattatcagg gcagatgcat ggtctagtta tgctggacaa tgaaggtaaa acattgagaa
     6781 actctataat atggtgtgac caacgaacag taaaagagtg tgaggagata actgatttag
     6841 taggtgaaga aagactgatt gaaataactg ctaatccagc gcttacagga tttacagcat
     6901 caaagatatt atgggttaga aacaatgaac cagatatata tataaatact aataaaatac
     6961 ttcttccaaa agattatata agatacaaat tgacaggtga atatgctaca gaggtatcag
     7021 atgcaagtgg tatgcagttg ctcgatataa gaaaaagaga ttggagcgat gaagtactag
     7081 aaaagttaaa tatagataaa aatcttttag gtaaagttta tgaatcacaa gagattacag
     7141 gttatgtgac tagagatgta gcaagtttga cagggcttaa agaaggaaca atagtggtag
     7201 gaggagctgg agaccaagca gcaggagcta taggaaatgg aatagttaaa gatggagtag
     7261 tttcatctac tataggtaca tcaggtgtag tatttgctta tacgaaagag ccaaaaattg
     7321 ataaagaagg tagagttcat actttctgtc atgcaatacc aaatacatgg catgttatgg
     7381 gagttactca aggagctgga ttgtcattaa aatggtttaa agataatttc tgtcaaagtg
     7441 aagtggaagt ttcaaattct ttaggtgaag atgtatatga gattataaat aatcaagtaa
     7501 gtcaagtacc aacaggatgt aatggattgc tttacttacc atatatgatg ggagaaagaa
     7561 ctcctcatct tgacccttat gcaagaggtg tgttctttgg attatctcca atccattcta
     7621 aaaaagaaat cgcaaggtca ataatggaag gtgtttcata tagtcttaaa gactgtatgg
     7681 atataataga gaatcttaat atagacgtta atgaagtaag agcctcaggt ggaggtggaa
     7741 aaagtaaagt atggagacag atgcaagcag atatgtttaa tcaagatgta tatactataa
     7801 attcaagtga aggaccagca cttggagttg caattcttgc attagtagga gctggaatat
     7861 atgaaaatat acaaaaagct tgtgatgcta taataaaaac ttcaactaaa ttagaaccaa
     7921 taagtgaaaa tgtagataca tacaagatat atcatagatt gtataaaaga atatacaaat
     7981 ctttgaaaga tgactttaag ttattagatg aagtagttaa tacaaaaata aataattaaa
     8041 ttagcttaaa aagaaaaata aagatagaaa atagttttaa attaagaaaa catacgacat
     8101 atattatatg tgtaaaatat taggaggaca tgaaatgagt gaaatattta aaggaatagg
     8161 acagataaaa tttgaaggag taaaatctga caatgaatta gcatttagat actacaatcc
     8221 tgaacaagtg gtaggtaata agactatgaa agaacactta agatttgcaa tgagctattg
     8281 gcatacttta tgtggagagg gtaatgaccc atttggagta ggcacagtag agagaccttg
     8341 gaataatgta acagacccaa ttgagatagc aaagattaaa gttgatgcag gatttgaatt
     8401 tatgtctaag atgggaattg agtatttctg ttttcatgac agagatatag ctccagaagg
     8461 aagagattta gaagagacaa ataagatatt agatgaaata gttgagtata taaaagttaa
     8521 tatggaaaag acaggtataa agttattatg gggaactgcc aatatgtttg gaaatccaag
     8581 atttgttcat ggtgcatcta caacatgtaa tgcagatgta tatgcatatg cagcagctca
     8641 ggttaaaaaa gctatggaaa taactaagta cttaggagga gaaaattttg tattctgggg
     8701 tggaagagaa ggttatgaaa ctttacttaa cactaataca gaacttgaaa tggataactt
     8761 tgcaagattc ttacaaatgg ctgtagatta tgctaaggaa ataggattca ctggccaatt
     8821 cttaatagag cctaagccaa aagagcctac taaacatcaa tacgattttg atactgctac
     8881 tgttttagga tttttaagaa agtataatct ggataaatac ttcaaaatga atatagaagc
     8941 aaaccatgca acacttgcag gacatacttt ccaacatgaa ttaaatatag caagaataaa
     9001 caatgtttta ggtagtatag atgcaaatca aggagaccta ttattaggtt gggatacaga
     9061 ccaatttcca acaaatatat atgatgcaac acttgctatg tatgaagtgt taaagcaagg
     9121 tggaatagca ccaggaggat ttaactttga ctctaaagta agaagagcat cttttgaggt
     9181 agaagactta ttcctagctt atattgcagg aatggataca tttgcaaagg gattattaat
     9241 agctcataaa ttattagaag atgaggtatt tgaaaacttt actaaagaaa gatatgcaag
     9301 ttttagtgaa ggtataggta aggatatagt agaaggtaag gttggattta aagagttaga
     9361 atcttatgca ctgcaaatgc cagttataaa gaataagtct ggaagacaag aaatgctaga
     9421 agctatactt aacagatata tatatgaagt agatacaatt tctaataagt aattgtgtag
     9481 taattaaact tggatgtttg aataaaataa caggatatgt tcttgttttt attctaaaac
     9541 ggacaaagat aagtatttgt aaaaattctc ataatcatga taaattaagg atatagactt
     9601 aagtataatt agtttatatc cttaaaattt ctataaaaaa taatcatttc tatagttatc
     9661 aatattttta attatcaata cctttctcaa ctaataaata aaaatcagta aagtatctaa
     9721 atttaagtca ttataatgga taaatttttt agcatatctt ttttagtata tcactgataa
     9781 gaatttactt tcaggatata gcagtaaaat agatatttga atataattgt tattttataa
     9841 ctttcatgaa ataattttga aaatacaaat aaaaattttt aaatagaata atagtggaat
     9901 atatcttcaa gaatttcttt taactgttta ggtgataata aaataatgat acaatgtatt
     9961 aaaatataaa atctgaaagt ggatgagaat tacatgaaaa agaaagtatt atttttagta
    10021 attattttag catttataat cttattaggt aattctactt attctcaaaa gaatcagttg
    10081 tctgaagagg aaaaggtgga tgactttaat tatgtgtata atgttgtaaa gacaggatat
    10141 ccatatctag gtgtaaataa gagacttaat aatattgatt ggttagcaaa taaggatgaa
    10201 tatacaaaac gaataaaaaa cactgaaaat gatgaagaat ttatagaaga actgtcatct
    10261 atattatctg atttaaataa taaacacaca gaagtaatag ataataagaa aagatatgaa
    10321 ctttttaaga aatcatactc taataatgat tggtatgatt ttcttaatga taaaaaagtt
    10381 gtggatagat atgactctat gaatcttaaa ataaaaatgc cagatgatat ttttgtaaaa
    10441 aaagaattaa ttttaaagga tgttattaag ggtcagattg gatatatgta tttaccatca
    10501 atgtcatcaa agaatggctc tataaataat gatttaaaaa taattggaga ttatattaac
    10561 actttagaaa aacataaagc tttaattatt gatataagag gtaatttagg tggaagtgat
    10621 aggtattggc agggaatcgt atctaaactc ataaaaaatg atgtcaaaat aaatggttat
    10681 agaatttata gaaataataa tgaaatagta aaaaaatata caaatgcaag aaatataaaa
    10741 ttaaatcctg tagaaaattt accagcaagt gtaaaggaaa atgcacctaa agaaattata
    10801 aaagatttta atgattttga agatacttcc tatactataa aatctacaga tgattcaaaa
    10861 tttaagggta atatatactt acttgttgat agaaaaatat attcatcatc agagtcattt
    10921 gcaatgtttt gtaaagaaac taaatttgcg actttagttg gtgaaactac tggcggagat
    10981 ggagggtgta tagaccctgt attgtttaat ttaaaaaata gtggtctaat agttagaatg
    11041 gcaagttgta tgtatttaaa taagaatgga atatgtgatg aggaatttaa aactattcca
    11101 gaatttaaag taaaagattg taaaaggact gctgatttta aagatgataa ttgcataaga
    11161 aaagcactag aacttgaaaa tattaattat tagataaaag aataaaatta aaactacatt
    11221 tttttaaaca tcaattatag cagtattttt tatagtcaat tttatgggct aaatagtaaa
    11281 tataagaata cgctgtaatt taatgttgaa tattggtata atatttatga cgatttttaa
    11341 ttaaagggga taatagttta tgagattaga agaaatgatt tttttaaatt ataataaatt
    11401 gaatgataca gatttattaa tatggaaata tataactgca aataaaaaac attgtagtaa
    11461 gatgtcaata aatgaacttg ctatgaaatg taatgtgtct cgtgcaacta ttacaagatt
    11521 tgttaaaaaa ttagatttaa aaggtttcag tgagtttaaa gtattattaa gctgggaatc
    11581 agaaaaggtt catgtcatag aaaatcagtc atttgatatt gcatgtaatt caataattaa
    11641 gtatgttgaa gatcaaaaaa ataaagatta tgatgcattg tgtaaacttc tttatgaatc
    11701 aagaacaata tacgcttatg gttcaggtga tattcaaaat gtggttgcaa aacagataaa
    11761 gagaatgttc ttatcatgtc aagaaataat atatgatttt gagggaagaa catttgatga
    11821 ttctttttac aatattgtca gtaaaaatga tgttatgatt attatatccc taagtggtga
    11881 gaatgagaat acacttaata ttgctagaag attaaaatta ataggaacta aaataatttc
    11941 aattacagag tttaaaaata atacattgac tgatttaagt gatgaatcaa tttatatttc
    12001 agcgactaat atctcatttt tacaaatgca ccctagttat aaattgacaa tgttatattt
    12061 tatattgatt gagttattat ttattaaata tagtatttat aaaaggcata gaatgattac
    12121 agaagggatt gattgcaaat tgtagtcatt ctttttttta tgagaatttg aacataaaaa
    12181 tgtgcaaaaa cttatttgac tgtgaataag ctccaaatgt accctaaaga ttgtatatat
    12241 ttattaacct gttaaaataa tagatatcta aggccaaaga taaactaaaa atatgaggag
    12301 gggagtaatt tatatgatga agaaaataca gcgttttgga ggggcaatgt ttacaccaac
    12361 actattattt gcttttgccg ggataatggt tggattctca attgtatttc aaaatcagtc
    12421 aattatgggt tcacttgcta caccagaaaa tatttggtac caattttggg gtgtaatatc
    12481 tagtggagct tggatggtat ttaatcaatt accattatta tttgctattt ctttaccaat
    12541 agcacttgca aaaaagcaac aagcaagagc ttgtatggag gctttagcaa cgtatttgac
    12601 ttttaattat tttgtaggtt ctatgttaag tttttgggga aaaagctttg gagttgattt
    12661 cgcagctgaa ataagtgctg gaagtggact tgtaagcatt gctggtatta aaacattaga
    12721 tactggtatg gttggtgctt tgttaatatc aggtattgtc atttatattc ataatagatt
    12781 ttatgataaa gaactaccag attttattgg attatttaga ggctcatcac ttgtagttgc
    12841 aatttgtttc tttgttatga taccagttgc attattaact tgttttattt ggcctcatat
    12901 tcaaaatgtt attagacatt tgcaaacgtt ttttataaat agtggaaata taggagtttg
    12961 gtgttatgca ttcttacaaa agattttaat accaactgga ttacatcatt ttgtatatgc
    13021 accaatttgt tatgatagtg ttgttgttcc aggtggtaca tcagtatatt gggctactca
    13081 tattcaggac tttcaaactt cagctaagac tctaaaggaa atgtatccaa tcggattttc
    13141 attatctgga ttatcgaagg tgtttggttc attaggtgta tttggtgctt tctatgtaac
    13201 tgcaaagcca gaaaaaaaga aaaaagtatt aggtttaatg attcctgcta ctcttactgc
    13261 agtattaact ggaattactg agccgttaga atttacattt ttatttgtag caccactttt
    13321 attcctagta catgcatttt tagatgcatg cttgcaaacg atttcgtttg ccttaggagt
    13381 tgttggtgat tttggtggag gaattattaa ctgggttgta ttaaattggc ttcctcttgg
    13441 aatgtatcac tggaaggtat atgttgttca agttgttgta gggattatat tttcatttat
    13501 ctggttcttt ttatttactt tcttaattaa gaagtttgat atgaaaactc ctggtagaga
    13561 ggaagataat gaagaaacaa aactatatac taaaaatgaa tacttagaaa ctaaagatga
    13621 aaaaggaaat aaattgagta aagcctctca acaagcatct gaatacataa aattagttgg
    13681 tggagcagaa aatgttgtag atgttacaaa ctgtgctacg aggttgagat taaccttgaa
    13741 ggatgactca attatttcta aagaagaaga ttttaaggta gtaggtgcac atggtttagt
    13801 gcataatgga aaagctattc agattattat tgggctttca gtaccaagtg taagagaaga
    13861 atttgaaaat ttattataaa ggagaaggaa agatggaaag aaaaagacag aggatactaa
    13921 ttgctggtgg gggtagtact tatactgctg gaattgtaac aatgttaatt gaaagtgttg
    13981 ctaaatttcc tattgaatca attaaattat atgataataa tgatgagaga caaagaaaag
    14041 ttgctgaagc atgtgcaata attgttagag agaaaaatcc agaaattaaa tttagttata
    14101 caacaaatcc agaagaagca tttactgata ttgattttgt aatggcacaa attcgtgttg
    14161 gtttatatgc tttacgtgaa caagatgaaa aaattccatt aaaatatggg gttgttggtc
    14221 aagaaacttg tggggcaggt ggaattgcct atggattaag aacaataggt ccaataatcg
    14281 aaatgattga ttatatggaa aaatattctc caaatgcttg gatgcttaat tattcaaatc
    14341 cagcagctat agttgcagaa gcatgtcgtg tattaagacc aaattcaaga attattaata
    14401 tttgtgatat gccagtatgt ttagaggaaa tatttgctag aattttaggt ttaaattctc
    14461 gtaaagactt tgatgttaga tattatggat tgaatcattt cgggtggtgg acaagtatta
    14521 aggacaaaga aggaaatgat ttaatgccta aattacagga atattgtgct aaaaaaggat
    14581 atgaggaatt tacacctcaa ggtcaacaca aagaatctag ttggttagaa actatgagag
    14641 cagcaaagga cttattagaa atagaaccga ctacattacc taatacttat ttaaaatatt
    14701 atttaatggc tgatgaaact gttgaacatg ctaatccaaa ttatacaaga gcaaatgaaa
    14761 ttatggatag aagagaaaag gatacattcg aagaatgtga aagaattata aaaaatggca
    14821 cagcaaggga tacatggttt gatgcaagtg aacattctca atttattgtt gaattagctt
    14881 gtgcattagc atttaatact caggaaagat ttttactgat tgttcctaat aatggagcta
    14941 ttgaaaattt tgcagatgat gcaatgattg aaattccttg ccttgttgga aaagatatgg
    15001 ttgaacctat gtctataggt aaaattccaa ctttccaaaa aggtttaatg gaacaacaag
    15061 ttgcttctga aaaattagct gtagaagcat ggatagaaaa aagttatcaa aaattatggc
    15121 agtcgtttac tatgagtaaa actgttccaa gtgcaaaagt agcaaaagaa atcttagatg
    15181 aaatgattgt ggctaataag gatttctggc caacttttaa ataaggagaa caaaaacatg
    15241 ataacaaata aaataaaaaa tataacagca gtattaaaag atgaaaatat atttctttca
    15301 gtggttatgg tttgttttct aatattacct gttttatata tagtgtattt agaattgaca
    15361 tcattattta caaacatttc attttcacaa cttttaaaca atgatgttaa tgcaagttta
    15421 aatttaatct ctaactttag tgcagtatac agtgcatatg ttatttacaa tataaaaaag
    15481 aataaagaga taaaagaaaa catgattgct ttctatttag tcttattagg gcagatattc
    15541 caaatttaca caataactat tgttatcatg tttacctata tttataaatt tgttggaatt
    15601 aaacaaatca taagcagtat taaagaacta caatggggaa agagctataa ggctcttttt
    15661 tcctcaattg cagtatgctt aatatcgtta tttgtaatga taattagaat aaagttaatt
    15721 atgtagagga gtgtctataa tgttctttaa aagaaaaaaa gaagcaagta caattaattc
    15781 atacattgat ggaaaaataa ttaatcttgg agaaattcct gataaagtat tttcaactaa
    15841 ggtaatggga gatggatttg ctatgatagc aaaggacaat attcttaaat caccgactaa
    15901 aggagtaata agttttattg ctcaaactaa ccatgctata ggaattaaat tgcaaaatga
    15961 tgttcaaatt cttattcata ttggattgga tagtgcaaca acaaaaaaag aaaactttaa
    16021 attagaagtt aaacaaggtc aagaagttaa tatgggggaa aagttagtag aaattgataa
    16081 taagttactt gaaaatactt tatatattcc tttagttatt gtagagaatc ctaatacggt
    16141 aaaatatcat ctttcaaact taaatcagga aggaaatagt ggtattaaag ttatcaattt
    16201 tgattgatta tgattaataa agcgattaaa tttaaattta tcatattcta ttttttctca
    16261 tatattattt ttgcctttgc ttcaacaaaa ttcacacctt tcttatcaaa attaggttac
    16321 tcagcgtttg aaagaggaat tatattatcg agctatgcaa taaccaatat attatttcaa
    16381 ttattatttg gagtattagc tgataagtat caaacaatga aaaaaattgt tttaattagt
    16441 ttatcagttt atggaattgc atcagttatt atgttttcta ctgaatctgc aacttttata
    16501 atatacttat tacttgttgc tttatcagga ggattattaa atacttgctg tagtttatat
    16561 gatacatgga taataggttg tggagataat attaataatt atttgtcatt tattaagact
    16621 tttgggtcga ttggatgggc tattggaagt atggtagcat cttacttgat tcttagattc
    16681 tcatattttg gattatctgt agcgattggt ttaattgtag tattacttat tataaatata
    16741 ctattattac ctgatattga aaagattaat aagaaaataa atgtaactaa taaagatatt
    16801 attgaattat taaaagataa aaaatatatc ttactagtat gtattttatt cttattatac
    16861 tcaatggtgg tagcaaataa ctgtactgta atagataaaa tgatagcttt aaatgctaca
    16921 aattctcaga tatctttaaa gtggtcttta cagtcattat tagagatacc aacatatctg
    16981 gctggttctt atttattaaa aaaatatagc agtttaaact tattaaaatt aagtgctatt
    17041 atgataacag ttcagtttct attatttgct ttaacaaatg attcaaatat cattattata
    17101 ctgtgtgttt ttcaagtgtt ttctactcct ttaattttaa ttacttcaaa aagattaata
    17161 tttgaagttt ctcctaaaaa acttagaagt agtagccaat taatagcttt aagtatattt
    17221 atgggaggtt catctctgat tattccaact gttgctggtt ttttgagtat taatattggg
    17281 tataattata cattaatgat attaagtctt tttggatgct tagcttatgg cttgatagtc
    17341 ttattgaatt atcttcaaga ataattgttt taatatatag tagtaatatc acttgtttat
    17401 agtacctgta tttgccacaa tcaaggttgt atttttggtt ttttatagag aagaattatt
    17461 gtatagatta gaggcttctt aggggcttct tttctttgtt tatacaaaaa agtgaatcat
    17521 gcttttattt taagatattt ccactataat tataatagtc ttccatatgt tacaaaagtg
    17581 taatgctaac agttaaaatg taatatttac ttaaggattt atttatataa aacctttaaa
    17641 ataaatatat attaaaaaat tagcaactgg aggctattat tatgaaagaa atattaaaaa
    17701 taaaaaatat atcaaaagat tatggaataa aaggatttaa aacaaatgtg ttaaaaaata
    17761 tatctcttac agtcaatgaa ggtgatttta tagcaataat gggtccatct ggtgcaggaa
    17821 agacaacatt gctaaattta atgtctacac ttgacaaaca aacttcagga gaaataatac
    17881 tagatggaat taatatatca aaagtaaaaa ataatgaact atcaaaatta agaagagaaa
    17941 aaatagggtt tatatttcaa gactataatc tattagacaa tatgaaacta atggacaata
    18001 tagcacttcc tctagcatta ggaaaaaaga aaagcaagga gatagaagct aaggtatttt
    18061 ctatagctaa aaaatttgga ttagaaaatc atttagataa atatccatat cagttatcag
    18121 gaggacaaaa acaaagaggc gcagcagcta ggtcattaat tacaaatcca gcagtcatat
    18181 ttgctgatga accaacaggt gctttggact ctaaatcagc atatgaactt ttagaatcat
    18241 tagaaaaaat aaacagagaa aacaacgcta ctattataat gattacacat gaccctctaa
    18301 cggcaagtta ttcaaatgaa gtatatatga taaatgatgg aaatataaag tgtaaattaa
    18361 acaaaggaaa tagtagaaaa gaattttatg gaaaaattat ggatatgcta gcctctatgg
    18421 gaggtgagat gtaagtgagt atacttggaa ttgcttataa taattttaag agtaacataa
    18481 gaacttatct agcatttttt atatctatgg ttttttcagt agttgtgctg actaattttg
    18541 aattattaag atatggagat gctataaatg tgttacaaga ggaaaataaa aaatttacac
    18601 tatcagtgtt agtagctgta ataatgatac tatcagtatt tttatttttc tttatatggt
    18661 atgcaacaaa tatatttttt aagaataggt ctaaagaaat tggaatattg tcctttatgg
    18721 gattagattt atatactata ggaaaaatat attttgtaga aaatatgctt ataggaataa
    18781 gttcttgtat aacaggaata attataggga taataacatc tagatttttt caagtagtga
    18841 taataaaatt atctggattt aatataaagg tatctaatgg acttagcata gaagctatac
    18901 tatatgctac tttaatcttt atttctatat ttataataat ggctgtaaaa ggttttataa
    18961 caatatgtag aagtagtgta ataaatctaa tcaatatatc taaaaaacaa gaaaaaatac
    19021 caaaggtagg aataggtata tatatattag ctatagtatc acttatagta ataggatatg
    19081 gatactatct ttctacgaat ataagaactg gagatataac tagtgtacta cctgttatag
    19141 gtataataat agtaggtact tatggcttat tcaaatcagt tatgcctgtt atatttgata
    19201 ttataatcaa aaataaaaaa atattattta atggaaataa tataattgcg ataaacaata
    19261 taaactaccg attaaataaa aactataaaa cttatgctat aatagctata attataacta
    19321 caacaatatc aactcttggt gcttcagttg ctatgaagca tatacataag aatgcccaag
    19381 aacaaagaaa tgtgtacact attagcatag tttcaatgga taaagatgaa gtgaataaaa
    19441 aacaaataga gaacataata aaagatacta ataagataaa gtactccgta aatccagaac
    19501 taacaatctt aaagaaaaca gatataaaag aagcttatga tacaacagat actataatga
    19561 aatatagtga tttttgtaat actcttaaag tgaatggcaa tgataaagaa ttaaaagagt
    19621 atggtgaaag attagttagt ggaaataatg taatagagct aaaaagtcct caaacgatag
    19681 catcaatagc tacaaaagaa gtcaagacaa aacttgatgg tcagttatat aatatttcaa
    19741 ggggagaagt aaaagttcca gtattaggga caggacttaa tcaaagtatt atagtggtaa
    19801 atgatgatac atattttaaa ctaagagata agggtcaaca agtatatttt tatggtgcaa
    19861 aggtatttca agaagaaaat agccaagtaa tgttcaacaa tattgaaaag aatttaagtt
    19921 taaaaaatgg atatattcag aatggatata aagctcaagg tgagagtcaa tggatgaagt
    19981 ttgcatatgt agtgcttgtg tttttattct tagtctttgc aattgtagct ggaagtataa
    20041 tatatatgaa aatatatagt gatgcatatg aagataaaga gaaatacaag attttattaa
    20101 aaataggtac tacagaaaaa gatataaata gagctatact taaagaagta gctatatttt
    20161 atactttgcc aatgttaagt gcaactatat ctagttattt tgcattgaga ttagcaggag
    20221 atttattgat gaacgattta tctggtatat atatactaag tttagtgata tgtttgataa
    20281 tattcgtaat atatggagca gtatctgtta ataagtttaa gagtgtagtg tataaaaatt
    20341 agttgatata ttaataaaga gaaaggagat tcttgtaata agagtctcct tgttgattaa
    20401 ctaaataatt tgggtaataa atttatataa aatatgttat gatattaata attcatggca
    20461 tagtgaaata ttaggataat ggagaatata caaatgtata gaattttatt agtagaggac
    20521 gatattgatt tatcaaaaga gattgcgtta gcacttgaaa aatggggatt taaagtagaa
    20581 ttaattgatg attttgaggt agtattagat gaatttatgg ataaaaaacc agatgtagta
    20641 cttttagatg taaatttacc attatataat ggattttact ggtgcgaaaa aataagagct
    20701 atttcaaatg taccattaat atttttatca tcaagagatt ctgatatgga tttaataatg
    20761 ggtataaata atggagcaga tgattatatt accaaaccat tttctataga catattggtt
    20821 accaaaataa atggtataat aagaagagtc tataattata gtgattctaa tagtattttg
    20881 tattgtgaag atttgatgtt tgatgtgggg aaagggatta taaaacataa gtataaggat
    20941 aaaagtatag agttgactaa aaacgaaata aaaattttaa cattattatt aaaaaataag
    21001 aatagggttg tatcaaggga aagtcttatg atgactctat gggataatga tgagtttgtg
    21061 acagacaatg cacttactgt aaatatgaat agattgagaa gtaaagttaa agaactaggt
    21121 tttgatgatt ttataaaaac taagaagggg ataggatata taatataatg ctagtaaaat
    21181 atttaaaagg taggtctaag tatattgctc tacctttagt tgtag
//
LOCUS       DAMJWP010000043        21189 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.044, whole genome shotgun sequence.
ACCESSION   DAMJWP010000043 DAMJWP010000000
VERSION     DAMJWP010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21189
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.044"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            173..1183
                     /locus_tag="PC700_001399"
     CDS             173..1183
                     /locus_tag="PC700_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HDF2580714.1"
                     /translation="MHEDKNDIFESIDTEDFDNKTEEECLELVRKNGLNLIYIKNQTE
                     EICLEAVRQNCNAIKCVQNQTEKICIEAVKQDWRMLEFVEESTEEICMLAINQDGTAL
                     KYIENQTEELCLRAVEKNGAALEYVKEQTEEICIEAVRNSEFGLARVKNQTEKICMEA
                     VKHCSYNLKWVENQTEEICMEAVRQNGLDLKFVKNQTETICLRAVRQNGMALEFVKEQ
                     TVGICLKAVRQYGMALKFVKEQTEEICTEAIKQDKRALSFVKGDKEKYKALYDNNEPF
                     AKRYVRNVIEKENRALIKKGEENAKIKIAGKLYDRGMSFEDISDIVEIEISKLKVSLG
                     VF"
     gene            1319..1807
                     /locus_tag="PC700_001400"
     CDS             1319..1807
                     /locus_tag="PC700_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2580715.1"
                     /translation="MKITVLYQTRSGNTERVAKLIEDGAKKVDGVDVKLMRLDNIDLD
                     YLNESKAVIFGTPTYLANTTWEVKKWFDEDSKKVNLAGKLGAVFATCDYICGGPDVAI
                     LTIVGHLMVKGMLVYSGGGSLGKPFIHLGHVHSIEGPELQDEKAIIFGERIATKAKEL
                     FA"
     gene            1983..3911
                     /locus_tag="PC700_001401"
     CDS             1983..3911
                     /locus_tag="PC700_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2580716.1"
                     /translation="MVWHIEYEIFSAIIVIFLMVYFFRSKFIPTLQNKIYCALLIFSF
                     LFILSNILGSFCLNNIDKIPIFITFLLNQIYLLLLPIPAALVSFYVMAIIYQDIRYMK
                     KRLLFLSIPLIICIILSITNPFTHILFGLSKETGYIRGCGYVATFASFFFYAIYSAFL
                     SFKYRKAMHESKIWAIRGFLIVSIVAIILQAIFLQYLLTGTACACSLLLTYLSMQNRG
                     LIIDDLTGVLNRQSFIQELDLNINTEQSGFIITIALDDFKFMNETFGTKNGDIALKEV
                     GKYLIQIHDVNHVYRIGGDIFSIVVNSQLGIEPDDIIRKIEERFKKPWLVEEISFNLA
                     TSIAVVYYPENAETTEDVIVAIDFSIHEAKRSESRRVVYADASISEKIKKKHVIKNCL
                     KEALKNDGFEVYYQPIFSNSRGRFTSAEALLRLEHKELGFIPPDEFIPIAEQTGLINS
                     IGLVVFEKVCRFIASDEFENLKLDNIAVNLSVVQCMQKNLADDLLYLMNKYNVSPTKF
                     KLEITETVAMGSFNIIKETMERLIDLGVKFALDDFGIAYSGVTNMLSLPFSVIKLDKS
                     LIWSMNEDSRHKLTVETIIALINKLNMKAVAEGVETIKCAEDLIAMECEYLQGYYFSK
                     PVPEDVFKNLLKENTFTS"
     gene            4086..4496
                     /locus_tag="PC700_001402"
     CDS             4086..4496
                     /locus_tag="PC700_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDF2580717.1"
                     /translation="MNKKKILVPIDGTERSMHSLEFIKGIFKKDEVEVEIMNVKELVF
                     IDGISLAEEIKNSENLGRRILDKAAEIMGEYDVKVHFTFGYPGDEIIRKAKEDNVDFI
                     VMTKSTKKGLTRMIGSVTASVVKQAKCIVMIVPE"
     gene            4518..4820
                     /locus_tag="PC700_001403"
     CDS             4518..4820
                     /locus_tag="PC700_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBC"
                     /protein_id="HDF2580718.1"
                     /translation="MNKKIVAVCESERSLNSLKTAANDLGFDLVYEIQENDNIKNELS
                     IYDIEDASIVLFVTSDSIESIEKIDRFIDREFYEVDPKYIIEDAKSIVSEILIDLN"
     gene            complement(5036..5728)
                     /locus_tag="PC700_001404"
     CDS             complement(5036..5728)
                     /locus_tag="PC700_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDF2580719.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCKEGILRAVPYSSPIVEGFKKVDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESEATKLAILNATSKELQKIKYTYLKSIEELDKVSHGLIEECYDADLQFHKEIILAS
                     HNPIFIKYYELIPKTVSSNQYFGFKYRNSLKDMISHHNKIMMAFDSKDSYLGYTSMYN
                     HLEDVIQLFQHD"
     gene            complement(5754..6941)
                     /locus_tag="PC700_001405"
     CDS             complement(5754..6941)
                     /locus_tag="PC700_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2580720.1"
                     /translation="MKKISNQKLLLLSIVTFFWFSQYVYIPFQNPYLSACNVSTNMIG
                     IIIGAYGISQCLLRIPVGVFADSINKHKIFILSGIFFAGLASLIRVFSPGGNGFLIAN
                     ILSGISSSTWISYMVHYTNFFSTEHQQKATSTIILVNNLGMLIGFVMSTLFYEKLGMT
                     ALCIMSVLSSVIGTILALNIKNHNSKKIPEKISIKKYLSICKDKRLVFFSFMALIQQG
                     IQMSTTMSFTTQILKNLGSSSTLIGLSSISYMVCAVASSGFASTKFCTKYGPKFWIPL
                     VFILVSAYCFLVPTIKNINIIFVLQVLPGISTGILLSYLTSESMKNVPKHQKSTAMGF
                     FQAIYAIGMSVFPMIVGKISEYTSIQYGYFILAIIAILASILSYTYYKKVDLTLNYDI
                     IQS"
     gene            7277..9154
                     /locus_tag="PC700_001406"
     CDS             7277..9154
                     /locus_tag="PC700_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2580721.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKTEGVITVVESMGQIQVVIGNKVKTVYDEVIKIVPQSNDVGKKNESQEKQSIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYVEKVLEKYIPEVIKIIMVPTLSLVVMLPATLFLFGPIGIYIGNVI
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGAA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AFGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSFIKVETKDGVIDITSPVEGECIELSEVKDDVFASKAMGEGIAVLP
                     TKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKKAGYDVTTPVIVNNTFDFGQVVSCKSSYVSTNDNIISLVLAG
                     N"
     gene            9466..10872
                     /locus_tag="PC700_001407"
     CDS             9466..10872
                     /locus_tag="PC700_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDF2580722.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLDFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNTVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEVLDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            10900..11748
                     /locus_tag="PC700_001408"
     CDS             10900..11748
                     /locus_tag="PC700_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2580723.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            12409..13101
                     /locus_tag="PC700_001409"
     CDS             12409..13101
                     /locus_tag="PC700_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2580724.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            13098..14033
                     /locus_tag="PC700_001410"
     CDS             13098..14033
                     /locus_tag="PC700_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2580725.1"
                     /translation="MKMDEQTIILFLFLLSIGLLFLSIFAFSKLGHIHKRLKDIEEIL
                     ADVQIGNENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIGQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVDDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQISVESEIGKYTVFTVSLPLI"
     gene            14181..15104
                     /locus_tag="PC700_001411"
     CDS             14181..15104
                     /locus_tag="PC700_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2580726.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRKFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAHAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            15088..15858
                     /locus_tag="PC700_001412"
     CDS             15088..15858
                     /locus_tag="PC700_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2580727.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVPKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVTVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPLLSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            15868..16650
                     /locus_tag="PC700_001413"
     CDS             15868..16650
                     /locus_tag="PC700_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2580728.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGIAVLLSLFSFLCTFILGLLFCNINISVTLIVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            17027..17563
                     /locus_tag="PC700_001414"
     CDS             17027..17563
                     /locus_tag="PC700_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HDF2580729.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            17595..17984
                     /locus_tag="PC700_001415"
     CDS             17595..17984
                     /locus_tag="PC700_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDF2580730.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            18021..18407
                     /locus_tag="PC700_001416"
     CDS             18021..18407
                     /locus_tag="PC700_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HDF2580731.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGSQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            18534..19976
                     /locus_tag="PC700_001417"
     CDS             18534..19976
                     /locus_tag="PC700_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDF2580732.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVENESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLIDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            20273..21088
                     /locus_tag="PC700_001418"
     CDS             20273..21088
                     /locus_tag="PC700_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2580733.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINNFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKRVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEIRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
BASE COUNT         7806 a         2488 c         3890 g         7005 t
ORIGIN      
        1 ccacatattt tgggcattgt atactggaat tagagtaatt aatttatttt ttaacataca
       61 aatgatttta tgaaaaaaaa caagactcaa aaatttttat attctttgat agaataaaat
      121 tgagagtaaa tttattgaat tttataaaat ttataagggg tgagtctaag atatgcatga
      181 agataaaaat gatatatttg aatcaataga tactgaggat tttgataata aaacagaaga
      241 agaatgttta gagctagtaa gaaaaaatgg attgaattta atatacataa aaaatcaaac
      301 agaagagata tgtctagagg ctgttagaca aaattgtaat gcaataaaat gtgttcagaa
      361 tcagacagaa aaaatatgta tagaagcagt taaacaagat tggagaatgt tagaatttgt
      421 ggaagagtca acagaagaaa tttgcatgtt ggctattaac caagatggaa cagcattaaa
      481 atatatagaa aatcagacag aggagttatg cttaagagcc gttgaaaaaa atggtgcggc
      541 tttggagtat gtaaaagaac aaacagaaga aatatgtata gaagcagtta gaaacagcga
      601 atttggattg gcaagagtga aaaatcaaac agaaaaaata tgtatggaag cagttaagca
      661 ttgtagctat aatctaaaat gggtagaaaa tcaaacagaa gaaatatgta tggaagcagt
      721 gagacaaaat gggcttgatt tgaaatttgt aaaaaatcaa acagaaacaa tctgtttaag
      781 agcagtaaga caaaatggaa tggcattaga atttgtaaag gaacagactg ttggtatatg
      841 cttaaaggca gtaagacaat atggaatggc attaaaattt gtaaaagaac aaacagaaga
      901 aatttgtaca gaagctataa aacaagataa aagagcactt agttttgtaa aaggagataa
      961 ggaaaaatac aaggctctat atgataataa tgaaccattt gcaaagagat atgttagaaa
     1021 tgtaatagaa aaagaaaaca gagcattgat taaaaaagga gaagaaaatg caaaaatcaa
     1081 aattgcagga aaactttatg atagaggcat gtcttttgaa gatatatcag atatagttga
     1141 gattgaaata agcaagttaa aagtgagttt aggtgttttt taaagtaata aatgtaggtg
     1201 ttttttatgg gaaatagtta ataaaaaaat aatcgactag ctctttgtag gacagcaaaa
     1261 ttagtgatat aatttgatgt ataataaatt atttttatta gataaggggg aattaaagtt
     1321 gaagattaca gtattatacc aaacaagaag tggaaacaca gaaagggtcg ctaagctgat
     1381 agaagatgga gctaaaaagg tggatggagt agatgtaaag cttatgagac ttgataatat
     1441 tgatttagat tatttaaatg aaagtaaagc agtaatattt ggaacaccta cttatttagc
     1501 aaatacaact tgggaagtaa aaaaatggtt tgatgaagat tcaaaaaaag ttaatttagc
     1561 tggaaaatta ggagctgttt ttgctacatg tgattatata tgtggaggtc cagatgttgc
     1621 gatacttact atagtaggac atttgatggt taaagggatg cttgtgtatt ctggaggtgg
     1681 ttctttagga aagccattta tacatttagg acatgtacat tcaattgaag gtccagagtt
     1741 acaagatgag aaggctataa tatttggtga aagaattgct actaaggcaa aagaactatt
     1801 tgcttaattt ctatatgcat aataatgatt agagtaaatt tagggtttta atggagattt
     1861 gaagtaaaag ttataaaata aaaaattttc cataaaatta ctcaagatga attataatat
     1921 aattataaat aaattatatt attaaaatat ttaaaatcaa acgtcaatag cgagggaagt
     1981 ttatggtatg gcatatagaa tatgagattt tttctgcaat tatagttatt tttttaatgg
     2041 tatacttttt tagaagtaaa tttataccta ctttacaaaa caaaatatat tgtgcacttt
     2101 taatattctc atttttattt atactttcaa atattttagg ttcattttgt ctaaataata
     2161 tagacaaaat tccaattttt attacttttt tattgaatca gatatactta ttgttattgc
     2221 caataccagc agctcttgta tcattttatg ttatggctat tatttatcaa gatataaggt
     2281 atatgaaaaa aagattatta tttttatcta taccacttat aatttgtata atcttatcta
     2341 ttacaaatcc ttttacgcat attctatttg gattgtcaaa agaaacaggt tatatacggg
     2401 gttgtggata tgttgcaact tttgcatctt ttttctttta tgcaatatat tcagcatttt
     2461 tatcttttaa atatagaaaa gcaatgcatg aatcaaaaat atgggctatt agaggttttc
     2521 ttatagtttc aattgtggca attatcttac aggcaatttt tttacagtat cttcttacag
     2581 gaactgcatg tgcatgtagt ttacttttga catatctttc tatgcagaat cgaggtctta
     2641 ttattgatga ccttactggt gtactcaata gacaatcttt tattcaagag ctagatttga
     2701 atatcaatac tgaacaatca ggatttatta ttacaattgc gcttgatgat tttaagttta
     2761 tgaatgaaac ttttggaaca aaaaatggag atattgcact aaaagaagtt ggaaaatatt
     2821 tgattcagat acatgatgta aaccatgtat atagaatagg gggagatata ttttctattg
     2881 ttgtcaatag tcaattaggt atagaaccag atgatattat cagaaagata gaagaaagat
     2941 ttaaaaagcc ttggctagta gaagagataa gttttaattt agcaactagt attgctgttg
     3001 tttattaccc agaaaatgca gaaacgacag aagatgtaat tgttgctata gatttctcta
     3061 tacatgaagc aaaacgttct gagtcaaggc gagttgttta tgcagatgcg tctataagtg
     3121 aaaagataaa gaagaaacat gttattaaaa attgtttaaa agaagcatta aaaaatgatg
     3181 ggtttgaagt gtactaccaa cctatatttt ctaatagcag gggcaggttt acttcagcag
     3241 aggcattact taggctagaa cacaaagagt taggttttat acctcctgat gaatttattc
     3301 caattgcaga acaaacagga ttgattaact caattggatt agtagtcttt gagaaggtat
     3361 gtagatttat agctagtgat gagtttgaaa atttaaaatt ggataatata gctgtgaatc
     3421 tttctgtagt acaatgtatg caaaaaaact tagctgatga tttactttat cttatgaata
     3481 aatataatgt atcaccaact aaatttaaac ttgaaattac tgagactgtg gctatgggct
     3541 cttttaatat aattaaagaa acaatggaaa gattaattga tttaggtgtt aagtttgcac
     3601 ttgatgattt tggaattgct tattctggag ttactaatat gctttcttta ccattttcag
     3661 taattaagtt agataagagt ttgatttggt ctatgaatga agatagtagg cataagctta
     3721 cagttgagac gattatagct cttataaata aattgaatat gaaggctgta gcagaaggtg
     3781 ttgaaaccat taagtgtgct gaagatttaa tagctatgga gtgtgaatat ttgcaagggt
     3841 attatttttc taaaccagtt ccagaagatg tttttaaaaa tttgttgaaa gaaaatactt
     3901 ttacaagtta acacccttta tttttttaat tacaactttg ttcattgaca ctgccagcga
     3961 tatattataa aattaacaca ataataaata ttaaatttat tatacatatt attaatatta
     4021 attaatggag taaagattta ttatatttta tttaattatc ctaaagtgag aaaggaagat
     4081 gtgacatgaa taaaaagaaa atattagtgc caattgatgg gacagaaaga agtatgcatt
     4141 ctttggaatt tataaaagga atatttaaaa aagatgaagt tgaagtagaa ataatgaatg
     4201 taaaagaact agtatttatt gatggcatat ccctggctga agagataaaa aattctgaaa
     4261 atttaggaag aagaatcttg gataaagcag cagagataat gggagagtat gatgttaaag
     4321 tacattttac ttttggatat ccaggagatg agataattag aaaagcaaaa gaagataatg
     4381 tagattttat agttatgaca aaatctacta aaaaaggtct tacaagaatg ataggttcag
     4441 taacagcaag tgtagttaaa caagcaaaat gtatagtgat gattgtaccg gagtagaatt
     4501 agaaaggtat ttgaaatatg aataaaaaga tagtagcagt ttgtgaaagt gaaagaagtt
     4561 taaattctct taagacagct gcaaatgatt taggatttga tttagtttat gaaattcaag
     4621 aaaatgataa tataaaaaat gaactttcta tttatgatat agaagatgca tctatagtgt
     4681 tatttgttac aagtgattct atagaaagca tagagaagat tgatagattt atagatagag
     4741 aattttatga agttgatcca aagtatataa tagaggatgc aaaatctata gtaagtgaaa
     4801 ttttaataga cttaaattaa tcgtgggtgt cgcactaaga atagagcgac gcctttttac
     4861 atattttaca taagaaataa agggctgtct caaactatag ataagactct ttttcattgt
     4921 tgaaatgatg aaaatatatt tgatttcata caaaaaagag tgcctcataa ctaaaaagtt
     4981 cactttgaga cactccattt ttatatgtta gttgcattta aggtaaaata gaagtttaat
     5041 catgttgaaa taactgaatt acatcttcta aatgattgta cattgatgta taacctaagt
     5101 aagagtcttt actatcaaat gccatcatta tcttgttatg gtggctaatc atatccttaa
     5161 gagaatttct atatttaaaa ccgaagtatt ggttagaaga aactgtttta ggtattaact
     5221 cataatattt tataaaaatt ggattatgag aagctaaaat tatctctttg tgaaattgta
     5281 agtcagcatc ataacattct tctatcaaac catgagaaac cttatccaat tcctctattg
     5341 actttaggta agtatatttt attttttgaa gttctttaga tgtagcattt aaaattgcta
     5401 gtttagtagc ttcactttcg ataagttgtc ttgctcttaa agattctaca taaaattctt
     5461 gtatgttatg atttttatag atttcaccat catcaaatag gtcaactttt ttaaatcctt
     5521 caactatagg acttgagtaa ggcacagctc taagtattcc ttccttacag agtatattta
     5581 tggcatttct tacaggtatt ctacttatgt taaataggtc agcaatcttt ctctcacttg
     5641 gtaatttact attaatttta aaagttccat ttagaatgtt taatttaata taatcaataa
     5701 ttttttcagt ggcatttaag cttttcataa aaactcctaa acatataatt tttttatgat
     5761 tgtattatat catagttaag agttaaatct acttttttat aatatgtata agacaggata
     5821 gaggccaaaa tagctataat agccaagata aagtatccat attgtattga agtatattcc
     5881 gatattttac caactatcat tggaaataca gacattccta tagcataaat tgcttgaaaa
     5941 aaacccattg cagtagattt ctgatgtttt ggtacatttt tcatagactc gctagtaagg
     6001 taagaaagga gtatacctgt agatatacct ggtagcactt gtagtacaaa tattatatta
     6061 atatttttta ttgttggaac taaaaagcaa taggctgaaa ctaaaataaa aactaaagga
     6121 atccaaaatt ttggcccata cttagtacaa aatttagtag aagcaaagcc agaagaagca
     6181 acagcacaca ccatgtaaga tatagaagat agacctatga gggtagaact agaacctaag
     6241 tttttaagaa tttgagtagt aaatgacatt gttgtagaca tttgaatacc ctgttgaatt
     6301 aatgccataa aggaaaaaaa tacaagtctt ttatctttgc agatagataa atatttttta
     6361 attgatattt tttcaggtat tttttttgaa ttatgatttt ttatatttaa ggctagaatc
     6421 gttccaataa ctgaagacaa tacactcatg atacataagg ctgtcattcc taatttttca
     6481 taaaataaag tactcataac aaatccaatc aacattccta gattgtttac caatattatc
     6541 gtactcgtag ctttttgttg atgttctgta gaaaaaaagt tggtatagtg aaccatgtaa
     6601 gaaatccaag ttgatgatga tattccagat aaaatgttag ctataagaaa accattgcct
     6661 ccaggagaaa agactcttat aagagatgct aaaccagcaa agaaaattcc agacagtata
     6721 aatattttat gtttatttat agaatcagca aatactccaa caggaatcct taataaacat
     6781 tgtgagattc catatgcacc tattattata ccaatcatat ttgtagatac attacatgca
     6841 cttaaatatg gattttggaa aggtatatat acatattgtg aaaaccagaa aaatgtaaca
     6901 atagaaagta aaagtaattt ttgattagat atttttttca tattatcctc catatggtat
     6961 ttttataatg caaataatat accatttaga ttttttaatc aagtactttc tctattaaaa
     7021 aaaatttaaa tatataatta ttatttacaa aaaaattata ctttatagtt gaattaagaa
     7081 aacgcttatg ataatataaa gttaacaaat ttgaatatat aacagtagac gtgttactgg
     7141 taatgcaggc aagatcgaat caatttttat agattatcta tagagtacta tgggtataat
     7201 ctatttattt ggttcgattt ttttgtttaa ttggccataa aacaaaaagc ctatagttga
     7261 aaatggagga ttttttatgg attataaaaa tatagctcaa gaaattctac taaatgtagg
     7321 aggaaaagag aatgtaaatg aagtgactca ttgtatgaca agacttagat ttaaagtcaa
     7381 aagcgcttct aaagtgaata aggataaatt atctaaaact gaaggagtaa taactgttgt
     7441 tgagagtatg ggtcaaatac aagttgtaat tggaaataaa gtaaaaacag tatatgatga
     7501 ggttataaaa attgtacctc aatcaaatga cgtaggtaaa aagaatgaaa gtcaagaaaa
     7561 gcaaagtata attaattcta tattatcagc agtagctggt atatttacac ctacaatacc
     7621 tgctatagca ggggttggta tgattaaagg tattttatca gttcttgcta tgtattatat
     7681 gaataaaaat ggagtagata taaaagaaac gcaaagttat attatcttaa atgccatggc
     7741 cgattctata ttctatttta tgcctattat actaggatat actgcggcta aagtatttaa
     7801 tgcaaataaa ataatatcta tggtattagg agctacactt tgttatccaa cttttactgc
     7861 tttgatggca ggtgaagaat ctgttagatt tttaggattg gcagtaacaa aagctacgta
     7921 tacatcatct gttattccaa taattattgc tatatgggct ctttcatacg ttgaaaaagt
     7981 attagaaaag tatatacctg aggtaattaa aataataatg gttccaactt tatcattggt
     8041 tgtaatgtta cctgcaacat tatttttatt tggaccaatc ggaatttata taggaaatgt
     8101 cataaacttt tcttataagt atatttatga attaagccca gcgttgtgtg gagcatttgt
     8161 aggtgggtta tggtgtgtgt tggttatatt tggtgctcat agagcattat taccaatagg
     8221 tataagtgat gttgctcaaa caggaagaca gaatttattg gcatttgcag gagctgcaaa
     8281 cttttcacaa gcaggggctg ctctaggagt attctttaaa actaaaaatc aaggactaaa
     8341 aacaatttct atgtctgcaa ctataacagc tttatttggt ataactgaac ctgcaatata
     8401 cggtgccaat cttagattaa aaaaacctat ggtatgtgcc gtaatatgtg gtgcatttgg
     8461 aggtgcgata atgggaatgg gtggagctta tggtaatgca tttgcaaacc aaggtgtttt
     8521 aactatacct gtatatgctg aagcaggtgc attaggtttc ttaagttatc taggtggttg
     8581 tgctatagca ttttttggtt cagctatttc aacttatcta gttggatttg aagatttgga
     8641 agagtcttct aataaggaaa attcatttat aaaagtagaa acgaaggatg gtgttattga
     8701 tataacttca cctgtagagg gcgaatgtat agaattgtca gaagtaaagg atgatgtatt
     8761 tgcttcaaaa gctatgggag aaggtatagc tgtgttacct acaaaaggag taataacagc
     8821 ccctactgat tgtgaagtag cttctttatt tccaacatta catgcaatag gattaaaatt
     8881 agataatgga gcagaaatgt taatacatgt tggaatcaat acagtagagt taaatggtaa
     8941 atactttaca aaacatgtta atcaaggaga tttagtgaaa aaaggtgata aacttatatc
     9001 gtttgatata gataaaataa aaaaggctgg ttatgatgta actacgccag ttatagtaaa
     9061 caatacattt gattttgggc aagtagtttc ttgtaaatct agttatgttt caaccaatga
     9121 taatataatt tcattagtat tagctggtaa ttaagttaat ttgactagag ataagtaaat
     9181 tgatttaaaa ggaaataagt aaatcaacaa ttaaatacat ctaaaagtaa gtttattgca
     9241 atgaattgtt aaagaaaata attaatatgg agtaaagcta ttgcaatgaa gtaacaaaga
     9301 aaataattaa tatagagtaa gtttattgta atgaattgtt aaagaaaata attaatatag
     9361 ggtaaatcta tctcaatgaa ataacaaaga aaagagttaa tatagagtga acttattgca
     9421 atgaatcaac aaaaaaaata atcaatataa aaaggagaaa tatttatgaa tacaggattt
     9481 ccaaaagact ttttatgggg agcatcatct tctgcatttc aagtggaagg agcttgggat
     9541 aaagacaata aaggaaaaac cgtagccgac tataattctt ttaaaaagtc tcatttacag
     9601 gcagatacta aagttgcatc agacttttac cataattatg aagaagatat tgaattaatg
     9661 aaagaattag ggatgaaaac atatcgtttt tcaatttcat gggcaaggat aataccagat
     9721 ggtgaaggag agataaacca gaaaggttta gacttttata ataagataat agacaaacta
     9781 atagagtgtg atattgagcc attcgtaact ttataccatt ttgaccttcc atttaaatta
     9841 gtagagaaat ataatggttg ggaaagtaga gaaactgtct atgcttttga aagatttgct
     9901 aaaatatgct ttaagcattt tggagatagg gtaaagtatt ggcaacctca taatgaacag
     9961 aatttaattg ttagagtgga ggagagaatt aatatatatg atgaaacaga ttcatggaag
    10021 atagataaaa tgcgtgctca gatggattat aatctttgct tagcacatgc attagcagtc
    10081 aatgcatgtc atgagatgat aaaagaaagt aaaattggag cagcagtatc atcatctgta
    10141 acatatcctc ttacttctaa gccagaagat gtatatgcag ctagaatgaa tgataatttt
    10201 aaagtgtact atatgcttga tatgcatcat tatggagaat atccagggta ttatatgaag
    10261 tatcttgaaa aaagaaatac agttcctcac atggaagatg gggataaaga gatacttaaa
    10321 aaagctaaaa tggattttat agctgtaaat tattatagga caaattgtgc agaagcttta
    10381 cctgaagatt cacaacatcc atttggatta agagaaggaa ctgttgattt tagtatgtat
    10441 ggactattta aaatgtcaat gaatcctaac ttagaggctt ctgagtatgg agcagcaata
    10501 gacccatcag gtcttagagt tgccttaaat gaatattggc aaagatatca cttacctgtt
    10561 ataataacag aaaatggtct aggagctaaa gatatattag aagatggaaa aattcatgat
    10621 gactatcgta tagactattt aagaagtcat ataaatgcat gtaaactagc tattgaagat
    10681 ggtgtagaga tgattggata ttgtccatgg tcatttacag accttttaag ttcttctcaa
    10741 ggttttaata agcgttatgg acttgtttat ataaatagaa ctgaccatga agttttagat
    10801 ttaaaaagaa tcaagaaaga tagtttttac tggtataaag aagttataga gaacaatgga
    10861 atagtaaaat aaacataaat atatattgag ggttttagta tgtacattac aaaggtatta
    10921 aataattcgt tgctacttgc aaaggatgat aatgatgaag aaataatatt gatggggaaa
    10981 ggcattggcc ataattataa ggccgggtct gagttaaata aaaaagatat agaaaagatt
    11041 tttgttcttc atgatgaaaa tataaaaaag agtattatac aattggcaac agaaatagat
    11101 gcagaatatt ttggtatagc tcaaatgata attgcatatg gaatagaaaa atataactta
    11161 aaacttatga accatatata tttggcctta actgaccaca ttgcctttgc agtgagacgc
    11221 tttaaagaag gtataatgat tgaaaatcac tatttgttcg aaatcaagga atttaatcca
    11281 aaagagtatg atataggaaa atatgctatt actgttttta aggaggttct tggtttagac
    11341 ctaccagaag aagagattgg taatatagca ggccatttta taaatgcaca acaagataat
    11401 ccttatagtg atagaaataa gagaagtgct aaaattgtaa atgctatttt gcaaatagtt
    11461 cattatcact tttctattgt atataataaa gagtcatttt attataggag atttgtaatg
    11521 catctaaagg ccttttcaca aagatttctg gctaatgaac catcaagaga aaaaatagat
    11581 tttatatatg agcaagttca aaaaaattgt aaggaagagt atgaatgtgt gaaaaaggta
    11641 ggagcttata tgtataagga atactccaga gaattaccaa gacaagagga gttatatttg
    11701 atgattcata ttcataagat tttaggagaa ttatgcgaaa atagttagaa tatcttaata
    11761 tgctagtaat attataaaaa attaaaaatt aactacactt taaatatgaa caattgcatt
    11821 acttaagggg gttgtcttaa aattgatttt tttaattaaa tttgaagctc aactcctttt
    11881 ttaaaccttt atatcattac aactttaatg tttatacgcc tgtactttag atgtatatct
    11941 actttttaca tttctcaaca tgcaaaccta ccattttttg cagtatgtta attgaaataa
    12001 accattgagt taaccctaaa tttattctgg acttatagga atagttccaa agatacttta
    12061 aaatattttc tggaataatt gcttatttca catatgtttt attgtgatgt tttgatttaa
    12121 taaattcttc aactctgtat atagtaaatg tattatcatg tttgttttat tcaagcacac
    12181 ttataatacg cttgattttt tcacaatgat gaaatatact aatgggtgtt ataggtataa
    12241 gaatcgaatt acacttgaag aagtaagtga taaatgagat ataaatcctc atatgataaa
    12301 ctactatttc attgaggaac atattctaag tgatgtcaag atactacaat atgactaaat
    12361 tcctagtgat aaaaaagtct acgaatgagt gtagaaaggg gaactaagat gaataaagtt
    12421 ttaattatag atgatgataa agatctttgt attttgttaa aaaagagtat ttcaattgaa
    12481 cagattgaag caaactattg ccattctggg agagatggac tggttgaact ttcaaaacaa
    12541 gattatcagt tagtagttct agacgtgatg atgccaggta tggatggttt tgaaactatg
    12601 gaacaaatac gaaaatatag cagtattccc atcttaatgc tgacatcaaa aagcgataat
    12661 tacagcaaag tgcatggatt gcgagctggt gcagatgact atctaaccaa accatttgaa
    12721 attacagaat ttactgctcg tgtactgtct ttaatccgac gttacacccg ttttaatcca
    12781 aaaaatgagg aaccacagca actttctttc aaaggtttgt tgattgacct tgaccagcat
    12841 acagtgacta ccaatggcga acctatgttg cttccaccca aggaatttgg tgtgcttctt
    12901 tactgtgcaa ggcatcaggg taagattttg acaaaacagc agatttatga agctgtgtgg
    12961 ggtgagccat atgtatatga tgacagcaat atcatggcta ttatcagtcg cctaagaaaa
    13021 aaaattgaac tagacccatc atctcccatc tatttgcaaa caataaaagg aattgggtat
    13081 caattcaaca cggaggtatg aaaatggatg aacaaacgat aattcttttc ttattcttgc
    13141 tgtcgattgg tctacttttt ctttccatat ttgctttttc caagcttggg catattcaca
    13201 aacgcctaaa agatatagag gagatactgg ctgatgtaca aattggaaac gaaaatcgca
    13261 aaatactaat taagccatgt gatgttatgg ctccattggt ttatcagctt aatgagattg
    13321 tctacgatta tgaaaataag ttactttctc tgaaaaagtc ggataaagct agcaaacaac
    13381 ttatgacaag tctatctcat gatgttcgta caccattaac aactctgatt ggttatctgg
    13441 atgctgtaca cagtggtata gttatcggac aagaacggga ggaatacctt gaaatcgcta
    13501 ggcgtagagc ctacgacctc aaggattaca ttgatgttct ttttgactgg tttcggttga
    13561 actctgatga atttacgctg tcaatagaat ctgtggaaat tgcagagttg tcacgcaata
    13621 ttttgaaaga ctggattcct atattccatg aaaagaaact gaattttgaa attgatattc
    13681 cagaaaatcg actaatggta aatctcgacc cagatggata ttcccgtgtg gttaataatc
    13741 tggttcaaaa tgttcttgct cacagtaagg ctcggcaaat caagattacc atgagtgagg
    13801 attccagaat ggtattactc cgtgttgatg atgatggtgt tgggattgcc agagaaaatt
    13861 taccacatat ttttgagcga ctgtataagt gtgataaagg acgttcagaa aaaggaagtg
    13921 gtcttggtct atctattgtc agtcaaatgg tggagcgaat gggtggtcag atttccgtag
    13981 aaagtgagat tggaaagtat accgtattta cagtgagctt accattaata taaaattgtt
    14041 tttcccccat gtgacatgta tacacagggg gattttttgt aagttataaa gttgcaaggt
    14101 taatacaagg ttgttgcaag gttaatgtaa gattgaggtg gtatgatagc tttaataaac
    14161 aaaggaggat tttattattt atgaataatt taattattga aacaaaaaat ctcactaagc
    14221 aatatggtgc tcaaaagagc gtttctaatt tgaatattca cgttcagaaa ggtcgcattt
    14281 acggattgct aggaagaaac ggtgcgggta agacgacaac tatgaaaatg ttattgggtc
    14341 taacaaaacc aactactgga gaaattaaaa tatttggtca ggacattcac aataacgaga
    14401 agaagcttct tcctcgaatt ggatgtttga ttgaatcacc agggttttat cctaatctga
    14461 caggaacaga aaatctcaaa atctttgcaa ggcttagagg aataccaaaa aaaaatgcag
    14521 tagaatcagc actggatgta gtcggtttgc caaacaatga caaaaagtta ttttctcaat
    14581 actctcttgg tatgaaacag aggttggcga ttgctcttgc cattatgcat gacccacaac
    14641 tgttgatttt ggacgagcct atcaatggac ttgatcctat tggtattgca gaagtaagaa
    14701 agtttattcg tgatctctgc acagaacgag gtaaaactat tttgatttcc agtcacattc
    14761 tttctgaaat tgccttgttg gcagatgaca ttggtattat tgaccatggt attttgttgg
    14821 aggaagaaag tcttgcccaa ttagaagaaa aaaacagtaa atacatacat tttgctgtat
    14881 ctgatgcagc acatgcagca aggcttctgg aaacccaact taacctgaaa aatttcaaag
    14941 tagcagataa ttacaatatt atgctatatg atacagatgt ttctgttgca gaaataaatc
    15001 gtgctttcat tctaaacaac attgaggtat cagaatctca tttatgcgaa gatactcttg
    15061 aagattactt caagaaagta acagggggtg aaggaattgc ttgatataat ttcatgtgaa
    15121 ttttcaaaac tcaagcggtt aaaatttata ctgatttcta ttttaggtgc ttgcttattt
    15181 ccaattcccg caacaatttt gattgcaaag gacaacttgc cctttgagca attgttcaaa
    15241 ttagttgtca attttggata ttttttgcta ttaccaattg tattaagtat tgttgctagc
    15301 caactatttt ttatagaaag agataacgat gtattaaaaa atcttgcaac agtgccagtg
    15361 ccaaaaggaa aactggctct ggcaaaattg gctgtgttgc tgtttatatc attgttctac
    15421 tccgttgcag ggttaggagc tactatcatt ggaggtttca ttgttggaat agtagagggg
    15481 gttactgtaa aattaggaat gagcattgct tttggaatta tgttttttgt tactgtactt
    15541 cctgttgtgg tactcattgt atatttcaat cgcagctata ttttttctat tatactttct
    15601 tttgtatatg ctattttcaa ttttagtata tccttaaata ttatcaattt tgaaccaaat
    15661 aacccactat taagcgtatt acctgctcct gttataatgc gttggtggat ggcattttgg
    15721 ggagatccca ctggcgaata cacggcactt agacagccat atttgctctc aactcctgct
    15781 tgtgtaggta ttttatgttt gataaccatt atagcagtcc tgctgatttg tatgatatac
    15841 aaaaagcagg agaattagga ggtatctatg cttgatataa ttactacaga atttcaaaaa
    15901 ataaaaagat ataatattct atggattggt gttgttgctg ttctgttttc agcactattg
    15961 gcagtttttc aggttgtaag ttcacacggc agtgaccctc taacgtatga gagctttgca
    16021 aacggagtta tctggaataa tttcagtctt agttttccat ttgggattac acttattgga
    16081 ggatatctta ttaaccgtga atatacagac caaaccttaa aaaacatcct aactgttcct
    16141 atttcattac gcaaattgct agtgggaaaa ttaattgcgg ttggaggaat tgctgtattg
    16201 ttaagtcttt tcagcttctt atgcacattt attttggggt tgttattttg caatatcaat
    16261 atatctgtta cactcattgt aaaatcctta gttcagattt tgtctgttaa tctgtattgt
    16321 tatgttgctg ttttgccgat tattgcttat tttggtcgta aacaaaatgc atttctgacg
    16381 ggtgtaggga ttgcatttgt atacgggttt tgtggtgtgt ttgttgctgg aagaaatctg
    16441 acagactttt atccaatcac agcaggtctt ggtctagttg gatacaacaa tggtgctggt
    16501 actgtttatc agccacttat cggtgctatg actttgattg taattctaat cttgacaact
    16561 attctacttg tatttacacc taattatgat aaagttatga ccatatctaa gaaaaaaggg
    16621 ataaagcata aaaagtattc tcataaataa cataaatatt gtttctatta tttaagttgc
    16681 tagacagtga aaagaaaaaa tctgtcgtac agtggctata tgctcatgta ttcaagaagc
    16741 tacagttttg atttttcata actgtagctt ctttttgtat ttattaaatc acggctatca
    16801 taatacttgc aataggtata gcgacaagag agagtactgt catgtgtgtt ttatatatta
    16861 cattaagtat attctaaaat atatgtttta aataatatat ttgaaaatag gaatatttaa
    16921 ataaaaaaaa taatattata tatagttgac aaaaattggc aaatgatata atatataaat
    16981 atagaaatag taatcattac tattattaaa aagggaggaa ttaattatga acttaaaagg
    17041 aactaaaaca gaaaagaatt taatggcagg atttgcagga gagtcagaag caagaaataa
    17101 atatacatac tacgcttcaa aagcaagaaa agaaggttat aatcaaatag ctgctatatt
    17161 tgaagaaact gctaacaatg aaaaggaaca tgctaaatta tggtttaaac ttttacatga
    17221 tggtatgcct tcaacagagg aaaatttaaa ggatgcagca gcaggtgaaa actatgaatg
    17281 gactgatatg tatgcaaaat ttgctaaaga agctagagaa gaaggatttg acaagatagc
    17341 ttatttattt gaagctgttg gaaaaataga gaaagagcat gaagaaagat acttaaagct
    17401 tttagaaaac ttaaatgaag gaaaaatatt taaaagagat gaagaagttg tttggcaatg
    17461 tcaaaactgt ggtcatgtgt atgtaggtac tgaagcacca gaaaaatgcc cagtatgtga
    17521 ccatccaaaa gcgtatttca atataaaagc tgaaaattat taaaaattat cattattaaa
    17581 aaggaatgtt aactatgaaa ttttctaaac aacgagaact gattttaaat gaaatattaa
    17641 ataatccggt tcatcctact gcggattacc tatatgaaaa cttaaaaaaa gataatccaa
    17701 atttaagctt aggaactgtt tatagaaatt tagctcagct aacggaacat ggctttataa
    17761 gaaaggttag tattccaggg tatccagata gatttgatgg tcgaatagat aatcactatc
    17821 atatcatatg tgaagtatgt ggagaggtat acgatttaga atccgaagtt cttaataatt
    17881 tacaagaatt aatatctgag gagacagaca taaaaataac atcttataac ataagtttta
    17941 aagggatttg taataattgt aaaaggtgta gccaagtagg ttagttttca aattctaaaa
    18001 ataattaatg aggtgagttt atgtgtagtg aacaaaaatt ttttatatgt gagacttgtg
    18061 gaaaccttgt aggaatgata caaagtggtg gtgtacctat tttctgttgt ggaaaaccta
    18121 tgaaggaact tgttcctaat acaacagatg cagcagttga aaaacatgta cctgtaattg
    18181 aagtggatgg aaataatgtt actgttaaag taagtagcac aactcatcca atgactaaag
    18241 aacatcatat agcatgggtg tatcttatga ctgaacaagg tagtcaacgc aaatgtctag
    18301 cagtagatgg agaacctgta gttaaatttg ctttgaatga tgatgataaa gtgatttctg
    18361 cctatgctta ctgtaattta catggattat ggaaggctga actgtaatat ttaaataaat
    18421 tgagtcaaag aggaggctat aaatgtattt tcatacaaat agcctcctat aaatttatat
    18481 aaattcaagt tttaattttg tttaatgtca atataatata aggagaaaat aatatgtcaa
    18541 tttataaatg tagtgtttgt ggatatattt atgatgaatc taagaatgat aaaacatggg
    18601 atgaattaag tgaagattgg gaatgccctg tatgtacaaa aggtcgtagt tattttggaa
    18661 agataagcac tgtttactat gaagaagatg aaaagatagc agaagacata gttgaaaatg
    18721 aatctaaatt aaatactgaa aaagagggag atttaaatta cttaagtaca tatttaagaa
    18781 gagatgatga agttgagaaa catatggata taattcacga aatggctgtt acaggaaaat
    18841 ctataataga acctatgaga actaaactac cagtaatttc ttgggatgat attttaatta
    18901 tgggtgctca attaaatcca ttacctttaa atgagcatga tgaagtaaat acaacaacaa
    18961 taataggaaa aaaagcaaag aaacctatga ttatagaaaa tccagtttat atttcccaca
    19021 tgtcatttgg agctttgtct aaagaactaa aaatagcttt agcaaagggt gcagcccaaa
    19081 ataagacagc tatgtgtagt ggtgaaggag gtatattacc agaagagaag gaagcttcat
    19141 acaagtatat ttttgagtat gtaccaaata aatatagtgt aactgaagaa aatcttaaaa
    19201 attctgatgc tatagagata aaaattggtc aaggaacaaa acctggtatg ggaggtcatc
    19261 ttcctggcga aaaagtaact gaagaaatag caaaagtaag aaataagcct gtaggtcaag
    19321 atgtaataag tccatcttgt tttgaggaaa tacaatccaa agaggattta aagaaactta
    19381 tagatgaact tagagaagtt tcagaagggc gtccaatagg tgttaaaatt tcagcaggtc
    19441 atattgaaaa agatatggaa tttatagctt atgctaaacc tgattttgta actattgatg
    19501 gtcgtggtgg agctactgga gcaagtccaa agttgcttaa agatgcaact tcaattccaa
    19561 caatatttgc tctttatcgt gctagaaaat acatagatac acatggatta gatatagatt
    19621 tggttataac aggtgggctt cgtatatcta ctgattttgc taaggcaatt gctatggggg
    19681 ctgatgcagt tgctattgca agttctgctc ttatggcagc ggcgtgtcaa caatatcgta
    19741 tatgtggttc tggtaagtgt cctgtaggag tagctactca ggatgaggaa ttgagaaaaa
    19801 gattacatat agaaaatagt gctaatagag tggcaaactt tttaaatgta agtttagaag
    19861 aacttaagac ttttgctaga atttctggtc ataaagatat acatgattta agtgttgatg
    19921 atttatatac tgttaactct gaaatatcaa attatacaaa catacagcat gtatagattg
    19981 aagtttgaaa ctattagatt ttagatagct ataaaagtaa atgataaata tattttataa
    20041 aagagagtgt cttaaaatga actttttagt tcataagtca ctcttttttg tatgaagcca
    20101 aatatattta tcattttagc aatgaaaaag aatcttatct ctttattttg agatagactc
    20161 cttctattta tgaaaattaa agatattaaa ttgtaaaata ttctgtgatg tcgtactatt
    20221 aaactaaaga ttctgtactt taaagtaaat aaaaatttaa ggagtgctta ttatggaaag
    20281 attgaagata caagttttag ttgacaataa tacttatatt gatagatact ttgttggaga
    20341 accagcagta agctattata ttgagattga tggaaatagg attttgtttg attcgggata
    20401 ttcagatgta ttcatatcaa acgctgaaaa attaaatata gatttaggaa atttaactca
    20461 tgttgttttt tctcatggac ataatgacca tacaagaggt atccagtttt tagaaaatag
    20521 gtatgattta tctacagttg aacttatttc acatcctaac tgttttatac ctagaaaaca
    20581 tggagagaaa agcataggag ctccattttc tgctgaggaa attaaaaata tttttatgta
    20641 taatcctaag gataagccat ttaatctaag taagaattgt gtgtttttag gtgaaatacc
    20701 ttctataaat aattttgaaa aaagagctaa aattggtaaa tgtaaaatag gagatttgtg
    20761 ggaagatgat tatgttttag atgattctgc tatagtttgt aaaactgata aaggtatttt
    20821 tattgttaca ggatgttctc atagtggaat ttgtaatata gtagagtatg ctaaaagggt
    20881 atgtggagat gatagagtta ttggtatact aggtggtttt catctttttg aattgaataa
    20941 taggttagat agtacaatac aatatttgga taaagaagaa ataagaatgt tgtacccttg
    21001 tcactgtgtt tcttttaagg taaaagctaa gatgaatgaa atattgaata taaatgaagt
    21061 tggagtaggg ctgaccataa gtatatagta tttgaaagta tataagattt aagttgaagg
    21121 ttaaaaagat attttctata taaagtatct tttttgttac gtttatttta agagaaatca
    21181 atatacttt
//
LOCUS       DAMJWP010000044        21001 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.045, whole genome shotgun sequence.
ACCESSION   DAMJWP010000044 DAMJWP010000000
VERSION     DAMJWP010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21001
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.045"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(<1..1390)
                     /locus_tag="PC700_001419"
     CDS             complement(<1..1390)
                     /locus_tag="PC700_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDF2580734.1"
                     /translation="MYKLIDKKDIDFLIDTCGEENVLVGSDINEDFSHDELGGIEKYP
                     EVLVNVLETEQVSKIMKYAYKNNIPVTPRGQGTGLVGAAVAINGGIMINLCKMNKILE
                     VDYENLTLTVEPGVLLMTIGQYVQDRDLFYPPDPGEKSATIAGNINTNAGGMRAVKYG
                     VTRDYVRGLEVVLPNGEIINVGGKVVKNSSGYSIKDLLVGSEGTLGIVTKAILKLLPL
                     PKKSISLLIPFPDLSMAIETVPKIIKSKSIPTAIEFMERDVILAAEEFLGKKFPDNTS
                     DAYLLLTFDGNSTEDIEKEYEKVANLCLENGALDVFISDTQERNDSIWSARGAFLEAI
                     KASTTQMDECDVVVPRDKIAEFIRYTHELQDKLKIRIKSFGHAGDGNLHIYILKDGMD
                     DNTWKIRLKETFDYMYKKSRELSGQVSGEHGIGYAKKEYLHESNSDAYMMLIKNIKLA
                     FDPKNILNPGKIY"
     gene            complement(1401..2621)
                     /locus_tag="PC700_001420"
     CDS             complement(1401..2621)
                     /locus_tag="PC700_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HDF2580735.1"
                     /translation="MSKIVVNQDKITDLKRILEICPFGAIEEKSGIVEISAGCKMCKL
                     CVKSGPKGAFEFIESSKVQINKDEWRGIAVYVEHHNGNIHPVTYELIGKAREMASKIK
                     QPVYCVFVGKDIKDKCSNLISYGVDEVFVYDEDEFKDFRIEPYSKAIENFIDKIKPTI
                     VLVGGTTLGRSLAPRLAARFRTGLTADCTILDIQSNTDLDQIRPAFGGNIMAHINTPN
                     NRPQFATVRYKIFSAPEKIENTTGKITLCKVEKKELKSKIKVLSVKEKNKEVGLEEAE
                     VIVVASRAIKKQEDMEMIYKLADKLNAQVAGTRPVIEAGWIDAKKQIGLSGRTVKPKL
                     IITCGVSGAVQFVAGMQGADYIVAINKDDKAPILDVAHLALIGDIYDIIPKLIEKIEN
                     NKDNNQKYMASAAK"
     gene            complement(2635..3423)
                     /locus_tag="PC700_001421"
     CDS             complement(2635..3423)
                     /locus_tag="PC700_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDF2580736.1"
                     /translation="MEILTCIKQVPGTTSVEVDETTGVLKRDGVDSKMNPYDLYALET
                     ALRIKEDKKANLKVLSMGPPQAKKVIEESFMMGADEGALISDRRFGGADVLATSYTIS
                     QGIKKMGKVDLIICGKQTTDGDTAQVGPEVAEFLDIPHVTNVTKLIEVKDESIVVEID
                     MPNDLQVCEIEYPCLITVEKDIFQPRLPSFKLKLNTKDREIPVYSLDDFEDKDENNYG
                     LNGSPTQVVRIFPPKPNTDKNIVRGNADELSFALVNKLEELKLV"
     gene            complement(3440..4714)
                     /gene="larA"
                     /locus_tag="PC700_001422"
     CDS             complement(3440..4714)
                     /gene="larA"
                     /locus_tag="PC700_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HDF2580737.1"
                     /translation="MVKLKVPYSKQGMILEIPDERFLGVLESKSDNYIEHKTQEEIVE
                     ESMNNPIGSNSLEELVKGKKNVVIITSDHTRPVPSRITMPIILRRIRNVNPEIDVKII
                     VATGFHRPSTREELIYKMGEEIVDNEDIVMHISTDDKSMCKVGILPSGGDLYINKLAY
                     EAELLIAEGFIEPHFFAGFSGGRKSVLPGIASAKTIMYNHCSDFIDSDNSRTGKLNNN
                     PIHEDMVYAAKVAKLAFILNVVIDKDKKIIASFAGDVEKAHTKGCRFVTELSKVNSIK
                     SDIVVTTNGGYPLDQNIYQSVKGMTAAEATCKDGGVIIMISACNDGHGGQSFYENVAN
                     ADSPKQLLDKIRSVDRVDTIPDQWEFQILARILSKYTVIMVTDKCNPEMIKKMHMKHA
                     FNFKEALEMANSIVGKESKVVVIPDGVSVIVK"
     gene            complement(4832..5545)
                     /locus_tag="PC700_001423"
     CDS             complement(4832..5545)
                     /locus_tag="PC700_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDF2580738.1"
                     /translation="MFNSIASKKVYEQVIEQIQYKILNGELKKGDKLLSERELSEQMN
                     VSRTSIREAIRVLETMGVIESRQGEGNFICTNIEKTLIEPLSMIFKLNNGTLEDILEL
                     RIILEIEIAKLASKRITSSEVIELKHIIDEMRVETNKKDNNRVLVLLDQKFHSKLATL
                     SKNYLIQSLFMTASKLFDGFIEDAREKIIAEPFNENILLKQHEAIYNAVVENDVELAC
                     EKAKEHMDFISKNYRKNEN"
     gene            5670..6929
                     /locus_tag="PC700_001424"
     CDS             5670..6929
                     /locus_tag="PC700_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HDF2580739.1"
                     /translation="MKKNKIIAFFAPAIIVGVLVLGIILYPNEAIKSAREGFSIWSNV
                     LVPSLLPFIIGANLIVDLKIVDIIGFIINPITRFVFNVSGKSALAFAISTVSGYPVGV
                     SLASEFRSNGQISKHEAQRLVSFCSTSGPLFIIGSVGTGMFQNSNLGYLMILCHYLGT
                     ILVGLFFRNYGNENLPKTKLDLKTNIKNVINIRNSSGNGFFVLFGNAVFNGVNTLLTV
                     GGFVIVFSVVFKILSLFNVISLIASVIYLPLSFLGVSKELCQAFVSGLFEITIGCNKV
                     SSITSSPEILRASLASFLIGFSGLSILAQCCTFLSKTDIDLKLYILSKFAHGVLAAIF
                     IFILYPIANSAVLVSSFADTYNVIYNNLIWFYYLSYYDTILQIVVIIYLISAVFIAKK
                     HMKTLSTNGKLMRYKNTFFIKKFSRKK"
     gene            7197..7763
                     /locus_tag="PC700_001425"
     CDS             7197..7763
                     /locus_tag="PC700_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2580740.1"
                     /translation="MATRPIEIDEYKKIMELLHTGFTYSENGVEKRFRKNPKVALALM
                     LEANLGLRISDILRLKIGNFKGNMLEINEKKTGKLQYRPVNKNIIESINEHARKYNLK
                     SNDYLVNIKTKAIQKQLRIICKHLNLYNISTHSFRKLYATTQFEKSNNNLELVKELLN
                     HSSVATTQRYIRVTQQAINEASENFFIG"
     gene            complement(7964..9169)
                     /locus_tag="PC700_001426"
     CDS             complement(7964..9169)
                     /locus_tag="PC700_001426"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HDF2580741.1"
                     /translation="MKIAILGYGTVGSGLIDLIENNTSKRDIEVVGILVKNKEKHKYK
                     KYFDKITTDIEDIFDKDIDILVEVIGGLNPAFDYVTRALNKKIHVVTANKDLLAEKGS
                     DLIELANLNNVSIKFEASVAGGIPVLKPLIESLEGNNIKSINAILNGTCNFILSKMYD
                     ENLPYDVALKQAQELGFAEVNPDADVLGYDSARKLSILSTLSYGKIVYWKDLLLEGIT
                     SIDEKDIEYAKKLNCKIKLVARSKYESGEVSGFVRPALVDNNNMLSKIDNEFNVVILV
                     GDSVGELSFVGKGAGRGATGSAVYADIIDIIDNRSSNIKSFSKGKLDLSGLIEDECSA
                     VVRFGGYNNKDGILECLDKYVDSYDIIDDEELAIFVSAKSEHEIDRVLEIVKRNNYSE
                     SAKKLLKID"
     regulatory      complement(9272..9378)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(9470..9838)
                     /locus_tag="PC700_001427"
     CDS             complement(9470..9838)
                     /locus_tag="PC700_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HDF2580742.1"
                     /translation="MLKGKQRAYLRSLANTLKPTTQIGKEGVTESFLEQLDGMLRTRE
                     LVKVTILENAGLDTKETANAVCEALRAEFVQAIGFKFTIYKRNIEEPKILFPGHEQAK
                     AKTKNNNVTKKGKPTKRAVR"
     gene            complement(9890..11167)
                     /gene="obgE"
                     /locus_tag="PC700_001428"
     CDS             complement(9890..11167)
                     /gene="obgE"
                     /locus_tag="PC700_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HDF2580743.1"
                     /translation="MFIDKARIFVKAGNGGNGSVAFRREKYVPAGGPDGGDGGRGASI
                     IFEVDLGLRTLMDFKYQKKYQAQNGGDGSKGKRAGKNGENLVLKVPAGTVIRDEATGL
                     VLADLKKEGDTAIVAKGGIGGKGNQHFANAVRQAPAFAKSGTDGEERWITLELKMIAD
                     VGLLGFPNVGKSTFLSVVTKAKPKIANYHFTTLTPNLGVVQTKFGDSFVLADIPGIIE
                     GASEGIGLGHEFLRHVERTKVLIHIVDISGLEGRDPIEDFDKINDELKLYNEKLSKRP
                     QVVVANKFDILEDESKFEKFKSELEGRGYTVFKMSAATRQGIDEVIAYVSKMLKEVED
                     VELVSEEEMYRPELDIGTEEELSIDIEDGVYVVTGKALRRIMYSVNFDDMESLQYFQK
                     AMESQGVFDRLREMGIEDGDVVKIYELEFEFYN"
     gene            complement(11278..11568)
                     /gene="rpmA"
                     /locus_tag="PC700_001429"
     CDS             complement(11278..11568)
                     /gene="rpmA"
                     /locus_tag="PC700_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDF2580744.1"
                     /translation="MLNMNLQLLASKKGVGSSKNGRDSISKRLGVKRFDGQLVTAGSI
                     IVRQRGTKIHPGTNVGKGSDDTLFALVDGTVKFERKDKKRKKVSIYPVAIAE"
     gene            complement(11582..11899)
                     /locus_tag="PC700_001430"
     CDS             complement(11582..11899)
                     /locus_tag="PC700_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HDF2580745.1"
                     /translation="MIKVKYYYNDDFLLQGFCLKGHADFAEIGYDIVCAAVTSNAIAV
                     INSLDKLQKIEFEKVVGEEGHIECIVKDTHVKDAQLLLNHFQLAVKEIKREYPKNIKI
                     LKK"
     gene            complement(11914..12225)
                     /gene="rplU"
                     /locus_tag="PC700_001431"
     CDS             complement(11914..12225)
                     /gene="rplU"
                     /locus_tag="PC700_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDF2580746.1"
                     /translation="MYAIVKTGGKQYKVSEGDVLFVEKLEANAGDVVTLNEVLACSKD
                     GELKLGSPVVEGASVQAKVVEQGKAKKVIVFKYKAKKDYRRKQGHRQSYTKIVVEKIN
                     A"
     gene            complement(12326..13747)
                     /locus_tag="PC700_001432"
     CDS             complement(12326..13747)
                     /locus_tag="PC700_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HDF2580747.1"
                     /translation="MKKIVIESLIGSQKTAVLEDERLTELFVEDNLNKKTVSNIYRGI
                     VKKVIPGIEACFVDIGFKKLAYLQLKKGSTIKSGQDILVQINKEEIGTKGAKLNTEIS
                     ISGRYIVYIPSNDRTTISNKITDEKERFRLKKITKAVNKENLGLIIRTEAQGCTHDEI
                     KKDIEELKLKYENILKEYKLGIGPKLLYKSLDFATKYVKDNVNDDIESIITNSYDKYS
                     ELKSILRGIDKTYVDKLCLEENRDVFDLYRIESKIEKLLNKKVWLKSGGYLIIEKTEA
                     LTVIDVNTGKFIGTGKLDETVYKTNLEAAKEIVRQLIIRDIAGIIIIDFIDMHKKKHQ
                     KEILHILEEEFNKDKRKAEVLGMTKLGLVEVARRREKESIDKYYLMSCPCCDGEQTIK
                     SVHYILDSIEKEIMRISEHTVYKNIMVEFNDFIFEQIKEYYMDIIDKIGEKYNIKISL
                     NANSTLKHNKTNVIFDKIVDNKM"
     gene            complement(13765..14466)
                     /locus_tag="PC700_001433"
     CDS             complement(13765..14466)
                     /locus_tag="PC700_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2344 domain-containing protein"
                     /protein_id="HDF2580748.1"
                     /translation="MSKIIRVKFKKEGDMIYISHLDLQRLLQRAFRRAEINLSHSQGF
                     NPHPKMSYGNALALGTESQGEYVDIEIEEDDLSVEEFLNKVSIQLPDGIDFIKAKEID
                     RQTPSLSSVIDYGEYLFNIDLKRPMTKEFVKRKVIDFMNNKEIIITKKNKKGKMVEVD
                     IRPMIRTFDVLNLEDEHITLTATIATGSKTNLNTNILIPKILEMFELDIDPLDVDILR
                     RDLYVLEDGELVTPM"
     gene            complement(14468..16312)
                     /locus_tag="PC700_001434"
     CDS             complement(14468..16312)
                     /locus_tag="PC700_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HDF2580749.1"
                     /translation="MNKVDLKKILKKVEKPARYLGNEINSIHKDTSNSELIRYAHCFP
                     DLYEVGMSHLGSHILYDVINKDEDVFCERVYSPAVDMENIMREKSIPLFALESREPIT
                     NFDFVTFTLQYELSYTNILNILDLANIPILKEERTLEDPFIMVGGPCAYNSEPLADFI
                     DVVILGEGEEVNLEVVNEYKEWKKNKTTREDFLYKISSIEGVYIPSFYDVKYNEDGTV
                     QSVTPNREGITKNPTKRIIKDVETVDYPEKLIVPYIDTVHDRIVLELFRGCTRGCRFC
                     QAGMIYRPIREKSVKRLKEILDKLVKNTGYDEISLSSLSTSDYSKLSELTDYLVDEYA
                     SNNIGISLPSLRLDNFSMEIADKIQQVRKSGLTFAPEAGTQRLRDVINKGVTEEDLEN
                     ATERAFEMGWNSVKLYFMIGLPTETYEDLDGIAKLAYKVIDIYRKVNGGKLKRSFSVT
                     VSTSTFVPKPFTPFQWHGQDTTEEVINKQRHLVNKLRNNNIKYNYHDSKTSLMEAVVA
                     RGDRRIGKVIYDAFRLGAKFDGWAEHFNLDIWKEAMEKNNLSIDFYAHRNRNYEEVFP
                     WDHIDVGISKKFLIREDENAKKEKITSDCRHNCNGCGINIHDIGRGLC"
     gene            16631..17824
                     /locus_tag="PC700_001435"
     CDS             16631..17824
                     /locus_tag="PC700_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HDF2580750.1"
                     /translation="MSKVFEVKKDIYFTGVVDEGLKVFDIIMETEFGTTYNSYLIKDE
                     KTVLFDTVKANFKDEFLSNLSEVTDIAKIDYVVIHHTEPDHAGSLKYLLDINPNIEVY
                     CTKAAKLYLDGQINRPFNCHVIKDGEILNIGKRNLRFITAPFLHWVDTMFTYIEEDKT
                     LLTCDAFGCHFASVDAEVVNSEDYLKSAKHYYDCIVKPFAKHVLSAVDKVVGLNIEFD
                     TILTSHGPMLTKDPMAAVKRYVEWSTEAVNTTNQNQVSIFYLSAYSNTLEMAKKIKEG
                     LDKEGAKAELYDLEDMTLTEMHDTLVVSKVILLGSPTINKTMVKPMWDLFSVIDPMAN
                     QGKIAGVFGSFGWSGEGITMAETLLKSMSFKMPVESLKKKFFPSEETLKECMAFGAEF
                     AKLVK"
     gene            complement(18036..19118)
                     /locus_tag="PC700_001436"
     CDS             complement(18036..19118)
                     /locus_tag="PC700_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HDF2580751.1"
                     /translation="MKKIRKLATKLLIATMVLTSCMSVTVYGMTAKDVTAKTPKSYVA
                     GTNSIYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKIRVAYNHIKNNTTYVNWNA
                     AEGANTAYTLVTKKGACSGMCRSLKALCDAMGIECYYVHSTTNDHQWNLIRFEDGRLY
                     HVDIDANKSAGADIIYKSLTHPLPFDKKAYPAVGESVKTQNPTPTVKKPTPAKPSAPV
                     GPGNHTMIADQEEAILDEVYGNNLRPKIERYEGVPCSLINSSINTIKFTSKNSNLDPS
                     RHTVSVIGFMNTPYGMVALAPDGVPVEYPFEYDKPFSLDFTGKNLSAAELSKKCKNNN
                     VIIKIEIGEIEPNSGRGQVRYARYVN"
     gene            complement(19682..20473)
                     /locus_tag="PC700_001437"
     CDS             complement(19682..20473)
                     /locus_tag="PC700_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580752.1"
                     /translation="MKLIDEYLDKLYKKCDNKSTIELKQEMRCHLIESANEFKLEGLD
                     EEEACKKAIERFDDGDEMQYELCNIIKELSLSLDRHKSIVMGFKKVLGYISIIAFLIS
                     GFMWYYNNSLQHNMYNLGKELDGEIKQLAERHDMTNIGEYKLELEKILDKDKYSKVKA
                     LRLYVIDMKDGNTNLSSSGLNANMVYEREADYNNISNFIQHLGYNGKDFLDKNGNIVN
                     PDIFLEYFFYFESEMLIPVAFAFGLLCIIAYFILRFKISLIKNNN"
     gene            complement(20477..20806)
                     /locus_tag="PC700_001438"
     CDS             complement(20477..20806)
                     /locus_tag="PC700_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2580753.1"
                     /translation="MQLNKEVLKGYIDILIVSILEKKDCYGYEIAKQVRERSEFELKE
                     GTMYLALKRMESKNLIKSYYSNEQSSGGRRKYYNLTNEGKDFLEIKKQEWRFIKKVMN
                     QFLGEVD"
BASE COUNT         6613 a         3797 c         2340 g         8251 t
ORIGIN      
        1 aatatatttt ccctggattt aaaatatttt tagggtcaaa agctaacttt atatttttaa
       61 ttaacatcat ataagcatct gaatttgatt catgtaagta ctctttttta gcatatccta
      121 tgccatgttc accagaaact tgaccactaa gttctctaga ttttttatac atatagtcaa
      181 aagtttcttt taatcttatt ttccatgtat tatcatccat accatctttt aaaatatata
      241 tgtgtagatt tccatctcct gcatgaccaa aactttttat tcttattttt aatttgtctt
      301 gaagctcgtg agtgtatctt ataaactctg ctatcttatc tcttggaaca acgacatcac
      361 attcatccat ttgtgtcgta gatgccttta tagcttctaa aaatgcacct ctggctgacc
      421 atatagaatc gtttctttct tgtgtatctg atataaatac atctaatgca ccattttcta
      481 aacataaatt agctactttt tcatattcct tttctatatc ttcagtgcta tttccatcaa
      541 atgtaagtaa aagataagca tctgatgtat tatctggaaa ctttttacct aaaaattctt
      601 ctgctgccaa tattacatct ctttccataa attctatagc cgttggtatt gattttgatt
      661 ttattatttt aggaacagtt tctattgcca ttgataaatc tggaaatggt ataagcaaac
      721 ttatactttt ctttggtaaa ggaagcaatt ttaatatagc tttagtaact attcctaaag
      781 taccttctga acctactaat aaatccttta tactatatcc agaactattt ttaaccacct
      841 ttccacctac atttataatt tctccatttg gtagtacaac ttcaagtcct cttacataat
      901 cccttgtaac gccatatttt actgctctca taccacctgc attagtgttt atattacctg
      961 caattgtagc acttttttca cctgggtcag gtgggtaaaa taaatctcta tcttgaacat
     1021 attgacctat tgtcatcaat aaaacacctg gttctactgt caatgttaaa ttttcataat
     1081 caacttcaag tattttattc attttacata gattaatcat tattccacca tttatggcaa
     1141 cagcagcacc tacaagacca gtaccttgac ctcttggtgt gactggtata ttatttttat
     1201 atgcgtattt cattatttta gatacttgtt cagtttcaag tacatttact aaaacttcag
     1261 ggtacttctc aatccctcca agttcatcat gtgaaaaatc ttcatttata tcacttccaa
     1321 ctaatacatt ttcttctcca caagtatcta ttaaaaaatc tatatctttt ttatcgatta
     1381 atttatacat ttctatcccc ctatttagct gctgatgcca tatatttttg attattatct
     1441 ttgttatttt ctattttttc aatcaattta ggaataatat cataaatatc acctattaaa
     1501 gctaaatgtg ctacatcaag tataggcgct ttatcatctt tatttattgc tactatatag
     1561 tcagcaccct gcatccctgc aacaaattga actgctccag atacaccaca agttattatc
     1621 aatttaggtt ttacagttct accactaaga cctatttgtt ttttagcatc tatccaacct
     1681 gcttctatta caggtcttgt tcctgccact tgagcgttta gcttatcagc taatttatat
     1741 atcatctcca tatcttcttg tttttttatt gctctgcttg ccacaactat cacttcagct
     1801 tcttccagac caacttcttt atttttttct tttacactca gtaccttaat tttagatttt
     1861 agttcttttt tttctacttt acataaagtt atctttcctg tagtattctc tattttttct
     1921 ggtgctgaaa atattttata tcttacagtg gcaaattgtg gtctattatt aggtgtgttt
     1981 atatgagcca ttatatttcc accaaatgct ggtcttattt ggtctaaatc agtatttgat
     2041 tgaatatcaa gtattgtaca atctgctgta agacctgttc taaatctagc ggcaagtctt
     2101 ggagctaatg acctacctaa tgtagttcca ccaactaata ctatcgttgg ttttatttta
     2161 tctataaagt tttctattgc ttttgaatat ggttctatcc taaaatcttt aaattcatct
     2221 tcatcatata caaatacttc atccacccca taagaaatca aattactgca cttatctttt
     2281 atgtcttttc ctacaaatac acaatacaca ggttgtttta tttttgatgc catctcacga
     2341 gctttaccta taagctcgta cgttacagga tgtatatttc cattatgatg ttcaacatat
     2401 actgcaattc ccctccattc atctttatta atttgtactt tactactctc tataaattca
     2461 aatgcgcctt ttggtccact tttgacacac aacttgcaca tcttacagcc agcacttatt
     2521 tctactatac ctgacttttc ttctatagcc ccaaaaggac atatttctaa aattctcttt
     2581 aagtcagtta ttttatcttg atttacaact atctttgaca tttattactc ccccttacac
     2641 caactttaat tcctctaatt tatttacaag tgcaaaactt agttcatctg catttcctct
     2701 tacaatattt ttatctgtat ttggctttgg aggaaatatt cttacaactt gagtaggaga
     2761 accgttaagt ccatagttat tttcatcttt atcttcaaag tcatctaagc tatatacagg
     2821 tatttctcta tctttagtat taagttttaa tttaaatgaa ggtagtcttg gttgaaaaat
     2881 gtctttttct acagttatta aacatggata ttcaatctca catacttgta aatcatttgg
     2941 catatctatt tcaacaacta tactttcatc tttaacttca attaattttg taacgttagt
     3001 aacatgaggt atatctaaaa attcagctac ttcaggtccc acctgtgctg tatcaccatc
     3061 agtagtttgt tttccacaaa ttattaggtc tacctttccc atcttcttaa taccttgtga
     3121 tatggtatag gaagtagcca atacatctgc tcctccaaat ctcctatcac ttattaatgc
     3181 tccttcatca gctcccatca taaaactttc ttcaattacc ttctttgcct gaggaggacc
     3241 catgctaagt acttttagat tcgctttctt atcttccttt attctaagag ctgtttctaa
     3301 agcatatagg tcatagggat tcatttttga atctactcca tctcttttta gtactcctgt
     3361 agtttcatca acttctactg aagtcgttcc aggaacttgt ttaatacatg tcaaaatttc
     3421 cataaagtac actccttttt tatttaacta taactgatac tccatcaggt ataactacta
     3481 ctttgctctc cttaccaact atactattcg ccatttctaa agcttcttta aagttaaatg
     3541 catgtttcat atgcattttt tttatcattt caggattaca cttatctgtt accattatca
     3601 cagtatattt agataatatt ctcgctagta tctgaaattc ccattggtct ggaatcgtat
     3661 ctactctatc gacacttcta attttatcta aaagttgttt tggagaatct gcatttgcta
     3721 cattttcata aaaagattga cctccatgac catcattaca tgctgaaatc attattatta
     3781 ctccaccgtc tttacaagta gcttcagctg ctgtcattcc tttaacagat tgataaatat
     3841 tttggtctag tggatatcca ccatttgtag ttacaacaat atcagatttt atagaattta
     3901 cttttgataa ctctgttaca aatctacatc ctttagtatg tgctttttcg acatcaccag
     3961 caaaggaagc aattattttt ttatctttat ctattacaac attcaatatg aatgctaact
     4021 ttgcaacttt agctgcatat accatatcct catgaatagg attattattc aatttcccag
     4081 ttcttgaatt atcactatct ataaagtcac tacagtgatt gtacattata gtctttgcag
     4141 atgcgattcc tggaagtacg ctttttctgc cacctgaaaa acctgcaaag aaatgaggtt
     4201 ctataaaacc ttctgctatc aaaagttctg cctcatatgc tagtttgttt atatataaat
     4261 ctccacctga aggcaatatc cctactttac acatagattt atcatcagta gatatatgca
     4321 taactatatc ttcattatct actatttctt ctcccatttt ataaattagc tcttcccttg
     4381 tacttggcct atgaaatcca gttgccacta taatcttcac atcaatttct ggattaacat
     4441 tcctaatcct tcttaatata attggcatag ttattctact tggtacaggt cttgtatggt
     4501 cacttgtaat tatgactaca ttctttttcc ccttaaccag ttcttccaga gaattagagc
     4561 ctataggatt attcatagat tcttcaacta tctcttcttg agtcttatgt tctatataat
     4621 tatccgattt tgactccaaa acaccgagaa aacgttcatc aggtatttct aaaatcattc
     4681 cttgttttga atacggtact tttaacttta ccatttttaa tctcccatta taataaacta
     4741 taaaattggt atgtggtcat accagtttca catttatatg atagtaccat tatctaccat
     4801 ttgcaagaat aatttatttt ataacaaact tttaattttc attttttcta taatttttac
     4861 ttataaaatc catatgttcc tttgctttct cacatgcaag ttctacatca ttttctacaa
     4921 ctgcattgta tatagcttca tgctgtttaa gcaatatatt ttcattaaaa ggttctgcga
     4981 ttattttttc tcttgcatct tctataaatc catcaaataa ttttgatgct gtcataaaaa
     5041 gactttgaat taaataattt ttacttaatg ttgctagttt actgtgaaat ttttggtcta
     5101 ataacaccag tactcgatta ttatcttttt tattagtttc tactctcatt tcatctatta
     5161 tatgtttcaa ttctataact tcagaagaag ttattctctt tgaagcaagt tttgcaatct
     5221 caatttctag gattattcta agctctaata tatcttcaag tgtgccatta ttaagtttaa
     5281 aaatcataga taaaggttct attaaagttt tttctatatt tgtacatata aaatttcctt
     5341 caccctgtct gctttctatc actcccatag tctctaatac cctaatagct tcccttatgg
     5401 aagttctact cacattcatt tgttcagaca attctctttc tgatagtaat ttatccccct
     5461 tttttaactc tccatttagt attttatatt gtatttgctc aattacttgt tcataaactt
     5521 ttttactagc tatactatta aacatttttt cctccaatat gataatcgtt gttgtaatat
     5581 atatctatta tatatcttaa accattacat tcatatattt ttattacttt taatataata
     5641 tcttataact caaatttaca ggtgatatta tgaaaaaaaa taaaattatt gctttttttg
     5701 ctccagctat tatagttgga gttctagttt tagggattat cctataccca aatgaagcta
     5761 taaaatctgc aagagaaggc ttctctatat ggtctaatgt tttagttcca tcactactac
     5821 catttataat tggagcaaac ttaattgttg atttaaaaat agtggatatt atagggttta
     5881 tcatcaatcc cataactaga tttgtattta atgtttcagg aaagagcgct ttagcttttg
     5941 ctatatctac agtatctggt tatccagtag gagtaagttt agcttctgaa tttaggagta
     6001 atgggcaaat ttctaagcat gaagcacaaa gacttgtgtc tttttgttct acatcaggac
     6061 ctttattcat aataggttcg gttggtacag gtatgttcca aaattcaaac ttaggttatt
     6121 taatgattct ctgccattat ctaggaacta tattggttgg tcttttcttt agaaattatg
     6181 gtaatgaaaa tcttccaaag acaaaattgg atttaaaaac aaatataaaa aatgtaatca
     6241 acattagaaa ttcaagtggg aatggttttt ttgttttatt tggaaatgct gtatttaatg
     6301 gagtaaatac attacttaca gttggtggat ttgtaattgt tttttcagta gtatttaaaa
     6361 tactatctct ttttaatgta atctcattaa tagcatcagt tatatatttg cctctttcat
     6421 ttttaggtgt tagtaaagaa ctctgtcaag catttgtaag tggtctattt gaaattacta
     6481 taggatgcaa taaagtatct agtataacaa gttctccaga aattttaaga gcatctttag
     6541 ctagcttttt gataggattt agtggattat ccatcctagc tcaatgttgt acatttctat
     6601 caaaaacgga catagattta aaattataca tactaagtaa atttgcgcat ggagttttag
     6661 ctgctatctt tatatttata ctttatccaa ttgcaaactc tgctgtgttg gtttctagct
     6721 ttgctgatac atacaacgtt atatataata atttaatctg gttttattat cttagctatt
     6781 atgatacaat tcttcaaatt gtagtaataa tttatctaat ctcagcagta tttattgcaa
     6841 aaaaacatat gaaaacttta tctacaaatg ggaaactaat gcgatataaa aatacttttt
     6901 ttattaagaa attttcacga aaaaaataaa actaataaaa cttaactaaa tacttccatt
     6961 tttttattat atttgtcata attttaataa cctttttaat tagtttaaca gcatagttat
     7021 ttcatcacaa tttaataatt ttattagaaa tatttttcaa aaaattctcc ttaatcacat
     7081 atatcacact tgtataagtt tactgggaaa tttttccatt atataatgtt tcatttatat
     7141 tatataataa ataccgacct aaacttacca aatcaaacta agaggagtgg ttgaaaatgg
     7201 caacaagacc tatagaaata gacgaatata aaaagataat ggaactatta catactggct
     7261 ttacttatag tgaaaatggc gttgaaaaaa gatttagaaa aaatcctaaa gttgcattgg
     7321 cactaatgtt agaagctaat ttaggtctaa gaatatcaga tattttaaga ttaaagatag
     7381 gtaattttaa aggtaatatg ttggaaataa atgaaaaaaa gacgggcaaa ttacagtata
     7441 gaccagttaa taaaaatata attgagtcca taaatgaaca tgcaagaaag tataatctta
     7501 aatcaaatga ctacttagta aatataaaga caaaggctat tcaaaaacag ttgaggataa
     7561 tttgtaaaca cttaaatctg tataatataa gtacacatag ctttagaaag ctctatgcta
     7621 ctactcaatt tgaaaagagt aacaataatt tagagttggt taaagaactt ttaaatcaca
     7681 gttctgtagc cactacacag cgatatataa gagtaactca gcaagctatt aatgaagcta
     7741 gtgaaaattt ctttattggt taattataac gtatacattt ttctattatc ttattaattc
     7801 accagatatt aaaaaagagt gtctcaaaat gaacttttta gttcatgaga cactcttttt
     7861 ttataaaatc aaatatattt ttattatttc aatactggaa aaaaggcttg tctctctatt
     7921 ttgagacacc ctattaaatt ttcataattc ttgcataaac atattaatct atcttcaata
     7981 attttttagc tgattcactg taattatttc tttttacaat ttccaaaact ctatcaatct
     8041 catgttctga ctttgcactt acaaaaatcg ccaattcttc atcatctata atatcataac
     8101 tatctacata tttatctaaa cattctaaaa ttccatcttt attattatac ccaccaaatc
     8161 taactactgc actacactca tcttctataa gtccacttaa atccaatttc ccttttgaaa
     8221 aagactttat atttgaactt ctattatcta ttatatctat aatatctgca taaacagcac
     8281 ttcctgttgc acctcttcca gctcccttac ctacaaaaga aagttcacct actgaatcac
     8341 ctactaaaat aactacgttg aactcattat ctatctttga aagcatatta ttattgtcca
     8401 ctaatgctgg tctaacaaat ccacttactt ctcctgattc atacttactt ctagcaacca
     8461 attttatctt acaatttaat ttttttgcat attctatatc tttttcatct attgaagtta
     8521 ttccttctaa taataaatct ttccaatata ctatttttcc ataagataaa gtagacaata
     8581 tagataactt tctagcacta tcatatccta aaacatctgc atctggatta acttctgcaa
     8641 atcccaactc ttgtgcctgt tttaaagcaa catcatatgg taaattttca tcatacatct
     8701 ttgataatat aaaattacaa gtcccattta aaatagcgtt tatgctcttt atattgttgc
     8761 cttccagtga ttctatcaat ggttttaaaa ctggaatacc acctgccaca gatgcttcaa
     8821 acttgatgct aacattattt aagttagcta attctataag gtcactacct ttttcagcca
     8881 gtaaatcttt attagctgtc actacatgta tttttttatt taacgctctt gttacataat
     8941 caaaagctgg atttaatcct ccaattactt caactagtat atcaatatcc ttgtcaaata
     9001 tatcttctat atcagtagtt atcttatcaa aatacttctt atacttgtgt ttttctttat
     9061 ttttaactaa aattccaaca acttctatat ctctttttga agtattattt tctattaaat
     9121 caattaatcc acttcctact gttccatacc ctaatattgc tatcttcact gtaacatacc
     9181 cccatcattt tgattatatt aaattattta tatatataaa tataccaata aaaaaagctt
     9241 gagtataaac tcaagccata tgacgggttt atactcatct ttcagcataa atgctgcagg
     9301 atttagcacc aatgtataat taaatatact ggttgccggg cttcacaggg ccagtccctc
     9361 tgccactctt gataagattg ctaattaaaa tttatgttta atagtataat acaatttctt
     9421 ccaattgtca ctattttttt aacttatttc cctaaaagga tataaactat tatctaacag
     9481 ctcttttagt tggtttaccc ttttttgtaa cattattatt tttagtcttt gctttcgcct
     9541 gttcatgtcc tggaaaaagt attttaggct cttcaatatt cctcttgtaa atagtaaatt
     9601 taaaaccaat agcctgcaca aattcagctc ttaaagcttc acatactgca ttagcagttt
     9661 ctttagtatc aagacctgca ttttctaata tagtaacttt tactaattct ctagttctta
     9721 acataccatc taattgctct aagaaactct ctgtaacacc ttctttacca atttgtgtag
     9781 taggctttaa agtatttgct aaagatctta aatacgccct ttgttttcct tttaacatat
     9841 tctcacctct ataattctat acttaaatta tctataatct atatttaaat taattgtaaa
     9901 actcaaattc taattcataa attttaacca catctccatc ttctattccc atttctctaa
     9961 gcctatcaaa aacaccttga ctctccatag ctttttggaa atattgaagg gattccatat
    10021 catcaaaatt tacagaatac attattcttc taagtgcttt acctgtaact acatacacac
    10081 catcttcaat atctatactt aactcttctt cagtaccaat atcaagttct ggtctataca
    10141 tttcttcttc tgaaactagc tccacatctt ctacttcttt tagcatttta gaaacatagg
    10201 ctattacttc atctatgcct tgacgagttg ctgctgacat tttaaatact gtatatcctc
    10261 tgccttctaa ttcactctta aacttctcaa acttactctc atcttctaat atatcaaatt
    10321 tatttgcaac tacaacttgt ggcctctttg aaagcttttc attgtacaat ttaagctcat
    10381 catttatctt gtcaaaatct tcaatagggt ctcttccctc taatccagat atatctacaa
    10441 tatgtatcaa tacctttgtt ctctcaacat gtcttaagaa ctcatgacct aatcctatgc
    10501 cttcagaagc accctctata attccaggta tatctgcaag tacaaaacta tctccaaact
    10561 tagtttgaac aacacctaga tttggtgtaa gtgttgtaaa atgatagttt gcaattttgg
    10621 gtttagcttt agttacaact gatagaaatg ttgatttacc tacatttgga aatcctaaaa
    10681 gaccaacatc tgctatcatt ttaagttcaa gagttatcca tctttcttct ccatctgtac
    10741 cagattttgc aaaagctgga gcttgtctta ctgcatttgc aaagtgttgg ttacctttac
    10801 cacctatacc accttttgca actatagctg tatctccttc tttttttaaa tctgctaata
    10861 caagacctgt agcctcatct cttattactg tacctgctgg tacttttaat actaaatttt
    10921 ctccattttt accagctctt tttcctttgc ttccgtcgcc accattttga gcttgatatt
    10981 ttttctggta cttaaagtcc attagagttc taagccccaa atcaacttca aatatgatgc
    11041 ttgctccacg accaccatct ccaccgtctg gaccaccagc tggaacgtat ttttctcttc
    11101 taaatgctac cgaaccattt ccaccatttc cagccttaac aaatattctt gccttatcta
    11161 taaacaaatt cttcacctac ttaattaaaa acttttaatt agcattacca aaaataccat
    11221 tataaattca tcagtattat aagaaaaagg agtgcctcaa cactcctttt atgtatatta
    11281 ctcagctatt gctactggat atatactaac tttttttctc ttcttgtctt ttctttcgaa
    11341 tttaacagta ccatcaacta atgcaaataa agtatcatca ctaccttttc caacattagt
    11401 tcctggatgt attttagttc ctctttgtct aactattata cttccagcag ttactaattg
    11461 accatcgaat ctcttaacac ctaatctttt agatatagaa tctctaccat ttttagaaga
    11521 acctacccct tttttagatg ctaataattg taaattcatg tttaacatat ggagtcaccc
    11581 cctacttttt taaaattttt atattttttg ggtattcccg tttaatttct ttaaccgcta
    11641 attgaaagtg atttagtaat aattgtgcgt cttttacatg cgtatctttg actatacact
    11701 ctatatgacc ttcttctcct actacttttt caaattcaat cttttgtaat ttgtctaaag
    11761 aatttataac tgcaatagca ttagatgtaa ctgcagcaca tactatatca tagcctattt
    11821 cagcaaaatc agcatgtccc tttaaacaaa aaccttgcaa caagaaatca tcattataat
    11881 aatatttaac ttttatcata acttaaccaa taactaagcg tttatttttt ctacaactat
    11941 cttagtgtat gattgacgat gtccttgttt tcttctgtaa tctttttttg ctttatactt
    12001 gaaaactata actttcttag ctttaccttg ttcaacaact ttagcttgaa cagatgctcc
    12061 ttctactact ggagaaccta attttaattc tccatcttta gagcaagcta atacttcgtt
    12121 taaagtaaca acatcacctg cgttagcttc taatttttca acgaataata catcaccttc
    12181 agaaacttta tattgctttc caccagtctt tactatagcg tacattaaat acacctcctc
    12241 ggcctcaaac tcgccagtac aaggtacttc atttgaagat ttaaaccttt actgtgcggc
    12301 attacaatta ttaattataa aatatttaca ttttattgtc aactatttta tcgaaaataa
    12361 cattggtttt attgtgcttt aatgtactat ttgcgtttaa agaaatcttt atattatatt
    12421 tttcaccaat tttatcaatt atatccatat aatattcttt tatttgttca aaaataaaat
    12481 cattaaattc taccattatg tttttgtaaa cagtatgttc acttattctc ataatttctt
    12541 tttcaatact atctagtata taatgtacag attttattgt ttgttcacca tcacaacatg
    12601 gacaagacat taaataatac ttatcaatgg attctttttc tctacgccta gcaacttcta
    12661 ctaaaccaag ttttgtcata cctaatactt cagcttttct tttatcttta ttaaattctt
    12721 cttctaggat gtgtaaaatc tccttttgat gcttcttttt atgcatatca ataaaatcga
    12781 ttattattat cccagctatg tctctaatta taagctgtct tacaatttct tttgcagctt
    12841 caagattagt tttataaacg gtttcatcaa gtttaccagt tccaataaac ttacccgtat
    12901 ttacatctat aaccgtaaga gcttctgttt tttcaattat taaataacct ccactcttta
    12961 accatacttt tttatttaat aatttttcta ttttagattc tattctgtat aaatcaaata
    13021 catctctatt ttcctcaaga cacagtttat caacataagt cttatctata cctcttaaaa
    13081 tagacttaag ttcagagtac ttatcataac tattggtaat tatagattca atatcatcat
    13141 tcacattatc ttttacatat ttagttgcaa aatctaaaga cttatataat aatttcggac
    13201 ctataccaag tttatattct tttaaaatat tttcatactt taatttcaac tcttctatat
    13261 ctttctttat ttcatcatga gtgcaacctt gagcctcagt ccttattatt aatcctagat
    13321 tttccttatt tactgcctta gtaatttttt ttaatctaaa tcgttctttt tcatcagtaa
    13381 ttttatttga tattgtagtt ctatcattgg aaggtatata gacaatatac cttccagaaa
    13441 tactaatctc agtgtttaat tttgcgcctt tagttcctat ttcttcttta tttatctgaa
    13501 ccaaaatatc ctgaccagat tttatagtac ttcccttttt taattgtaaa taagcaagtt
    13561 tcttaaatcc aatatctaca aaacaagctt ctattcctgg tataactttt tttacgattc
    13621 cacgatatat attggataca gtcttcttat ttaaattgtc ttcaacaaaa agttctgtta
    13681 atctttcatc ttcaagtact gctgttttct gggaccctat tagggactca ataactatct
    13741 ttttcacaac ttcacctctc gagcttacat aggagtcact aattccccat cttctaatac
    13801 atataaatct cttctcaata tatcaacatc taatgggtca atatcaagtt caaacatctc
    13861 taatatcttt ggtataagta tattagtatt taaattagtt tttgagcctg tagctatagt
    13921 agcagtaaga gtaatatgtt catcttctaa atttaaaaca tcaaaggttc ttatcatagg
    13981 tcttatatct acttcaacca tcttaccttt tttattcttt tttgttatga ttatttcttt
    14041 attattcata aagtcaatta cttttctttt tacaaactct ttcgtcatag gtcttttcaa
    14101 atctatatta aacaaatatt ctccatagtc tatcacagaa gaaagagaag gtgtttgtct
    14161 atcaatttct tttgctttga taaaatctat cccatctgga agttgaattg atactttatt
    14221 taaaaattcc tctactgata aatcatcttc ttctatttca atatctacat actcaccttg
    14281 actttctgtc cctaaagcca aagcattgcc ataactcatt ttagggtgag gattaaatcc
    14341 ttgagaatgt gataagttta tttcagctct tctaaacgct ctttgtaaca atctctgaag
    14401 gtcaagatgt gatatatata tcatgtcgcc ttccttttta aattttactc ttattatctt
    14461 actcatatta gcaaagcccc cttcctatat catgtatatt tattccacaa ccattgcagt
    14521 tatgtctaca atcagaagtg attttttctt ttttagcatt ttcgtcttct ctaattaaga
    14581 attttttact aatacctaca tctatatggt cccaaggaaa tacctcttca tagtttctat
    14641 ttctatgtgc ataaaaatct atagaaagat tattcttttc catagcttct ttccatatat
    14701 ctaaattaaa atgttctgcc catccatcaa atttagcacc tagtctaaat gcatcatata
    14761 taacttttcc tattcttcta tcgcctctag ctactactgc ttccatcaaa ctagtctttg
    14821 aatcatgata attgtatttt atattgttat ttcttaattt atttactaaa tgtctctgct
    14881 tattaataac ttcttctgta gtatcttgac catgccattg gaaaggtgta aatggctttg
    14941 gtacaaatgt agaagtactc actgtaacac taaaacttct ctttagcttt cctccattta
    15001 cttttctata aatatcaata actttataag ctaatttagc tataccatct aaatcttcat
    15061 aagtctcagt tggtaaacca atcataaaat acaacttaac actattccac cccatctcaa
    15121 aagctctttc tgttgcattt tccaaatctt cttctgtaac acctttattt ataacatctc
    15181 taagtctttg agttcctgct tctggagcaa aagtaagtcc agattttctt acttgttgta
    15241 ttttatcagc aatttccatt gaaaaattat ctaatctaag ggatggaagc gaaatcccaa
    15301 tattattaga tgcatattca tctaccaaat aatcagtcaa ctctgataac ttactataat
    15361 cactcgtact aagtgatgat aatgatattt catcatatcc agtattctta actaatttat
    15421 ctaatatttc tttaagtctt ttaacactct tttctcttat tggcctgtat atcatacctg
    15481 cttgacagaa tctacatcct ctagtacatc ctctaaataa ttctagtact attctatcat
    15541 gaacagtatc tatataaggt actattagtt tttctggata atctacagtc tcaacatctt
    15601 ttattatcct tttagtagga tttttagtaa tcccttctct attaggagtt acactttgaa
    15661 ctgtaccatc ttcattgtac ttgacatcat aaaaacttgg tatgtatact ccttctatgc
    15721 tagatatttt atataaaaaa tcttctctag tcgttttatt tttcttccat tctttatact
    15781 catttactac ttctaaatta acctcttcac cttcacctaa tattactaca tctataaaat
    15841 ctgctaatgg ttcggaatta taagcacaag gaccaccaac cattataaat gggtcttcca
    15901 aagttctttc ttcttttaat attggtatat tggctaaatc taatatattt aagatattag
    15961 tgtatgaaag ttcatattga agtgtaaaag tgacaaaatc aaagtttgtt ataggctctc
    16021 ttgactctaa tgcaaataat ggtatacttt tttctctcat tatattttcc atatcaactg
    16081 caggagaata cactctctca caaaatacat cttcatcctt atttattaca tcatataata
    16141 tatgactccc taagtgactc ataccaactt catataaatc tggaaagcaa tgtgcatatc
    16201 ttatcaattc actattacta gtatctttat gaattgaatt tatttcattt ccaagatatc
    16261 tagcaggctt ttctactttt tttaagattt ttttcaagtc tactttattc atttattaaa
    16321 attcctccaa acaagctctt tatttttatt cttatttata agtagtatta tacacgataa
    16381 aatattttct atcaaaaata taactaaaat attgaattta aaatacatat tattcacaaa
    16441 atagaatagc taaaaatatt taagaatatt ttctattaaa tttttattga aatacaattt
    16501 ttttacaaag tatgatatat tataaacata aaactattgt tttacatgac ttatcatgac
    16561 atcttttgtt tcattcacct caaaattggg tatgttactt atataatgat aaattttagg
    16621 agggttaaaa atgagtaaag tttttgaagt aaaaaaagat atctacttta ctggcgtagt
    16681 agatgaaggt ttaaaagtat tcgacataat aatggaaact gagtttggga caacttacaa
    16741 ctcttattta atcaaggacg aaaaaacggt tttatttgac actgtaaagg caaattttaa
    16801 agatgagttt ttaagtaatt tatcagaggt tactgatata gctaaaattg actatgttgt
    16861 aattcatcac acagaaccag accatgcagg tagtttaaaa tacttattag atataaatcc
    16921 taatatagaa gtttattgta ctaaagctgc taaactatac ttagatggtc aaataaatag
    16981 accttttaac tgtcatgtaa taaaagatgg tgaaatttta aatatcggta aaagaaatct
    17041 tagatttata actgctccat tcttacattg ggttgataca atgtttactt acattgagga
    17101 ggacaagact cttttaactt gtgatgcatt tggttgtcac tttgcaagtg ttgatgcaga
    17161 ggtagtgaat agtgaagatt atctaaaatc agctaaacac tactatgatt gtatagttaa
    17221 gccatttgct aaacatgttt taagtgctgt agataaggtg gtaggactaa acatagaatt
    17281 tgatactata ttgacttctc atggtccaat gcttactaaa gacccaatgg ctgctgttaa
    17341 aagatatgtt gagtggtcta cagaagctgt taatacaact aatcaaaatc aagtttctat
    17401 attctactta tcagcttata gtaatacttt agagatggct aaaaagataa aagaaggact
    17461 tgataaagaa ggtgcaaagg ctgaattata tgatttagaa gatatgacat taacagaaat
    17521 gcacgataca ttagtagtgt caaaagtaat attactaggt tctccaacta taaacaaaac
    17581 tatggttaaa cctatgtggg atttattctc tgttatagac ccaatggcta accaaggcaa
    17641 gatagcagga gtatttggtt catttggttg gagtggtgaa ggtataacta tggctgagac
    17701 tttacttaaa tctatgtcat tcaaaatgcc tgtagaatct cttaagaaaa aattcttccc
    17761 tagtgaagag acattaaaag agtgtatggc atttggtgca gaatttgcaa aattagttaa
    17821 atagccatta atcacacata atattattta aaataaatga ttttatattt aaaaataaat
    17881 taaaatggag ttgtctcaaa attaattttt ctaatctaat tttaagttca actccttttt
    17941 tatttcccaa aatgactaat ataatgataa atgcaccctc accaagaggg tacattattt
    18001 ataatctttt ctatttgtat acttgtatat ttatattaat tcacatatct agcatatctt
    18061 acttgccctc ttccagaatt aggttctatt tcaccaattt ctattttaat aattacatta
    18121 ttgtttttac acttttttga taattctgct gcagaaaggt ttttaccagt aaaatctaaa
    18181 gaaaatggct tatcatattc aaaaggatac tcaacaggta caccatctgg agctaatgca
    18241 accataccat atggtgtatt cataaaccct attacagaaa ctgtatgcct tgatgggtct
    18301 aaatttgaat ttttgcttgt aaacttaatt gtatttattg atgagttgat taaactacac
    18361 ggaactccct catacctttc aattttggga cgtagattat ttccataaac ttcatcaagt
    18421 attgcttctt cttgatctgc tatcatagtg tgattcccag gtccaactgg cgcagaaggc
    18481 ttagctggcg taggtttctt aacagttggc gttggattct gtgtctttac tgattcacca
    18541 acagctggat aggctttctt gtcaaatggt aatgggtgag taagactctt gtatatgatg
    18601 tctgctcctg ctgatttgtt ggcatcaata tctacatgat acaaacgtcc atcctcaaaa
    18661 cgaataagat tccactgatg gtcatttgtt gtactatgta cataataaca ttcaattccc
    18721 atagcatcac ataaagcttt caagctccta cacattccag aacaagcacc ttttttagta
    18781 accaatgtat aagcagtatt ggctccctct gctgcgttcc agttaacata tgttgtgttg
    18841 tttttaatgt gattataagc aaccctaatc ttagcatctg tggtcatgtt ctttgtaatt
    18901 ttcttgttca taaagtctgc tgttgcttgt gctaccgaat taagttgagc ttgtgataac
    18961 tttggaccat atatactgtt ggttccagct acatagcttt tcggtgtctt tgcagtaaca
    19021 tctttagctg tcataccata aactgttaca ctcatacatg aggttaaaac catcgttgca
    19081 atcaatagtt tagtagctaa tttcctgatt tttttcatat tagcattctc ctttataata
    19141 tattttactt tcaattccct accattaatt aagttcattt tagatttaaa atatggatta
    19201 tcaacatgtg tgcatagcaa ttcacgtggt catagaatat ttagatttct tggaaaatct
    19261 gttctatatt tcctatatag ttcgtacaca atccaactca caaacctgca tttttgatat
    19321 catcacaata aaatgataat aattaatata ttcaaaaatg ttggtctgta acaggtttgt
    19381 caattttcta attattggta atgacgagag tgaggagaat tgtcaataat acatttaaaa
    19441 ttaaaattgt atacacttca tacattctcc cctaacaaca ttatacagat tataatttgt
    19501 atattcaata cattttttgg aaggtataat tatatcaaaa tatgtgatta tgtatagata
    19561 caatccacta tggagaaata taaaagaaaa gagtataaca aacaataaaa aataagatga
    19621 ttcaaatttt attttgaaac atcttattta ctctacaaat taaaattatt tggtgaatta
    19681 tttaattatt attttttatt aaagaaattt taaatcttaa tataaaatac gctattatac
    19741 ataataatcc aaatgcaaat gctactggaa ttaacatctc cgattcaaaa taaaagaaat
    19801 attctaagaa aatatctgga tttactatat tcccattctt gtctaaaaaa tcttttccat
    19861 tatatcctaa atgctgtata aaattactta tgttattata atctgcttcc ctctcatata
    19921 ccatattagc attcaatcca gaagaactta aattagtgtt tccatctttc atatctatta
    19981 catatagtct aagagctttg actttactat acttatcttt atcaagtatt ttttcaagtt
    20041 ctaacttata ttcaccaata tttgtcatat catgtctttc tgccaattgc tttatttctc
    20101 catcaagttc ttttcctaaa ttatacatat tatgttgtaa actattattg taataccaca
    20161 taaatccaga aattaaaaat gcaattatac ttatgtaacc taatactttt ttaaacccca
    20221 ttacaattga tttatgccta tccaatgata aagatagttc ttttattata ttacataatt
    20281 catactgcat ttcatcacca tcatcaaatc tttcaattgc ttttttacat gcttcttctt
    20341 catctaatcc ttctaattta aattcatttg cagattctat aagatggcat ctcatttcct
    20401 gttttaattc tatagtactt ttattgtcac attttttata tagtttatct aaatattcat
    20461 ctattagttt cataaattaa tccacctctc ccaaaaattg attcataacc tttttaataa
    20521 atctccattc ttgtttcttt atttctaaaa aatctttacc ttcattagtt aaattataat
    20581 attttcttct tccacctgaa ctttgttcat tgctataata agatttaatt aaatttttag
    20641 attccattct tttaagtgca agatacattg ttccttcttt taattcaaac tcacttcttt
    20701 ctcttacttg ctttgcaatt tcatatccat aacaatcttt cttttctaat atagaaacaa
    20761 ttaatatatc gatataacct tttaacactt ctttatttaa ctgcattatg taccaccgcc
    20821 ttttcaccta ctactctata atgtatagta cttatattta ctataataat acatagtact
    20881 ctataataca aggtttatat tctacaatag acttaaaaat aataaatagc tgtatattag
    20941 tatttataga ttaaatacta atatacagct atttatcaaa ttttaaaaat aaatttttgt
    21001 t
//
LOCUS       DAMJWP010000045        20957 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.046, whole genome shotgun sequence.
ACCESSION   DAMJWP010000045 DAMJWP010000000
VERSION     DAMJWP010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20957
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.046"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            172..1233
                     /locus_tag="PC700_001439"
     CDS             172..1233
                     /locus_tag="PC700_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HDF2580754.1"
                     /translation="MRDFLKNYKSSIILLACTIIGVIVGLVLGPKASILKPFGDLFIN
                     ALLMIVVPLVFLSVSSGVASIKNMERFGKIMKSTAIVFMGTTLVIAFLGITSALLFNP
                     SKGLDVSSVKSLMVAQDAETLESKGTLLDQVVSSITVKNFGDLFSSSNLLQLVIFAVL
                     VGTAIAMMGDRGNTIRKFLSEGTEAILKVVSIVMLYAPIGLGCYTATIIGELGSKIIQ
                     GYVRVFVIYSLTAAMYFLLFYSLYAFLSAGKQGFRQFWRNAIKPIATALATSSSSACI
                     PSNLEASKNIGVPDDISETVIPLGINIHKDGSVLAVIIKVAFLSGILGSGMANPATIG
                     QLFITVFVVGVFMAPVAGG"
     gene            1246..1443
                     /locus_tag="PC700_001440"
     CDS             1246..1443
                     /locus_tag="PC700_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HDF2580755.1"
                     /translation="MLVVSIFGFPVEAIPLLTVITTITDIPNTILNTTGNTVSSMLVS
                     RLVEGKDWLIDKTAITTKKIS"
     gene            complement(1607..2335)
                     /locus_tag="PC700_001441"
     CDS             complement(1607..2335)
                     /locus_tag="PC700_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF2580756.1"
                     /translation="MKTNYRLHMQQGSDYLKKFLEKHIDLLKKIPLFEGIKSDELEEM
                     LECLGVVDKTYPKDSIIFSVGSEVTSIGIMLKGSAHIIKEDIEGNRNIVAELLPGDLF
                     GEVFACTRLHKSNVTVTTTSSCEVLFIKFKSVTGICSSACVFHNRLVENMLQLIAEKN
                     ILLHNKIELLSRKTTREKLLMYFSKQIEQTGSHQFTIPFSRNEMADFLCVDRSSMSRE
                     LGKMRDEGLLSFNKNKFEIFYFQK"
     gene            2476..3201
                     /locus_tag="PC700_001442"
     CDS             2476..3201
                     /locus_tag="PC700_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2580757.1"
                     /translation="MMRKIIKIDEGKCNGCGLCVEACHEEAIGMVNGKAKLLRDDYCD
                     GLGDCLPTCPTNAISFEYREAAEYDEAAVKANMEAKKAQKKTLACGCPGSQSKSINRE
                     VSNSTSISNDIVEIKGSQLNQWPVQIKLVPTNASYLKNASLLIAADCTAYAYGNFHNK
                     FMKNKVTLIGCPKLDEVDYAEKLTEILKENDIKNIVVTRMEVPCCGGIVNAVKTALQN
                     SGKMIPWQIVTISVDGKIVDGEI"
     gene            3261..4853
                     /gene="hcp"
                     /locus_tag="PC700_001443"
                     /gene_synonym="priS"
     CDS             3261..4853
                     /gene="hcp"
                     /locus_tag="PC700_001443"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HDF2580758.1"
                     /translation="MEKMFCFQCEQTAGGKGCTINGVCGKKGGTANLQDELTGVLIGL
                     SRATIGNKNRPTSETDKIMIEGLFTTITNVSFDDEAIKRQIEKTEVEKSKLVPRCSDC
                     STTCNRNDNYDMKDLWNDNEDIRSLKSLILFGLRGMAAYAYHAMVLGYTDKDVNEFFY
                     EGLIAVGGDLSIDELLGLVMKTGEINLKCMELLDRANTETYGTPEPTQVSMKIEKGPF
                     IVVTGHDLYDLHKLLEQTKDKGINIYTHGELLPAHAYPKLKEYKHLKGNFGTAWQNQQ
                     KEFDNIPAPILFTTNCLMPVKDSYRDRVFTTEVVAYPGMVHIDEDKDFSSVIEKALEL
                     GGYREDVHMTGINGGDTLTTGFARGTVSSVQDKVVDAVKSGAIRHFLLVGGCDGAKLG
                     RNYYTEFVKQAPKDTVILTLACGKYRFNDLDLGDIGGLPRLMDMGQCNDAYSAIKVAV
                     SLANAFECGVNDLPLSMVLSWYEQKAVCILLTLLHLGIKNIYIGPSLPAFLSKNVLDI
                     LVDKFALTPISTPEEDMKKILG"
     gene            5217..6122
                     /locus_tag="PC700_001444"
     CDS             5217..6122
                     /locus_tag="PC700_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDF2580759.1"
                     /translation="MKSSNFNILAFDGGGLKGALSISILERIVKEYPNLLNNINMFGG
                     TSTGSLIALGLAYGVSPKEIKELYSIENSKYIFNKSYAEILRPKYENKNLKEVLLSIF
                     PEELELKDLNKLVMIPSFYIGNEENAWKPVFYNNMPNSFTKTSKVVDVAMASSAAPVF
                     FPTYNRHVDGGIIATDPSLACIIHAIDSGFKLKNTRLFSIGTGYVYNSIKADTTEWGA
                     IDWIINKEPDLPIISITLEGNSQMSQLFSQKLLGDNYYRLNPKMEKDVAMDDCDALEY
                     LMSLGENCDIKDAFGWIKKKSFYRN"
     gene            6246..7199
                     /gene="msrA"
                     /locus_tag="PC700_001445"
     CDS             6246..7199
                     /gene="msrA"
                     /locus_tag="PC700_001445"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDF2580760.1"
                     /translation="MIKLATFAGGCFWCMVKPFDKYDGVIKVVSGYTGGHVENPTYQE
                     VCSGTTGHLEAIQITYDDDIISYDELLNIFWKQINPTDDGGQFGDRGTQYKTAIFYHD
                     EEQKNIALESREHLENSKIFDSKIVTDIIPATKFYEAEENHQDYYKKRPLNYNMYYKG
                     SGRYNFVKSHWDGNNINREELKKTLTPIQFEVTQNDMTEVPFENEYYDNNKEGIYVDI
                     VSGEVLFSSKDKFDSGCGWPSFSKPVIDKSIIERSDFSQGMYRIEVRSAKGNSHLGHV
                     FDDGPKELGGLRYCINSASLKFIPRENMEVLGYGEYLKLFE"
     gene            7352..8482
                     /locus_tag="PC700_001446"
     CDS             7352..8482
                     /locus_tag="PC700_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2580761.1"
                     /translation="MNLKDFNGKRLKSARIFRAKTIEQLSKETKINKKDLKAFEENKY
                     VPNIENTLKLSNILNFPKEYFYKNENINIVVEDSHFNPQSKLPRVEEISYREKIIIIH
                     KIYSFMENYIKFPKENLPNRKVMSDINISDIESLAYKTREFWDLNSTPIVNMVSLLES
                     KGIIISGMNVDRKGATIFTQKQRISKESKYLISLGNDKKSASIRNYTLACELGYIISS
                     ELRIPSKQFSEDEYACAFLLPKESFLKDLTHPEDLDYYVELKKKWIVPISAMIFRAYN
                     LEKINYKKYNYLMNEMDKKGWLIEEPLDKMKGSSPTYLKRAVELLIENKIMSVNSIVS
                     SLEEFGINLYPEDLELLMGLKKGLLSQEVNKKSKVIKFDGKK"
     gene            complement(8599..9567)
                     /locus_tag="PC700_001447"
     CDS             complement(8599..9567)
                     /locus_tag="PC700_001447"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDF2580762.1"
                     /translation="MAIKPRKISIVGSGHVGSHCGFSLITQGVCDELFMIDIDESKSK
                     AQALDLADAVSYLPHKVHIEKGTFSDCKDSDIVVISVADSSEGPLRRQNTTRLDLLRP
                     TIGMIKSIVKPIVDSGFDGIFVVISNPVDVVTNYIWEKSGFPKNKVIGTGTALDSTKL
                     RRILSEETGIAQQSIQAYSMGEHGDSQMVPWSHVSIGGKPILDMIKDNPNTYSNLDLP
                     SIVEKNKKTGISIINGKDCTEFGIGTALVEIVKAILHNEKKVLPVSTLLEGQYNERNV
                     FAGVPCVIGKDGIEEIIEINMTEYEQNEFNKSCSVLRECIALSKTL"
     gene            9902..10090
                     /locus_tag="PC700_001448"
     CDS             9902..10090
                     /locus_tag="PC700_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580763.1"
                     /translation="MNYHTKEELMEVLRVASSRIINCEKVQKKFSEETSHHTRFKNII
                     EAMYISKSLIMDEISKRD"
     gene            10350..11549
                     /locus_tag="PC700_001449"
     CDS             10350..11549
                     /locus_tag="PC700_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein AlaX-L"
                     /protein_id="HDF2580764.1"
                     /translation="MEKLYYTNQYIRDFTAEIIEIKEVENKYHVLLDKTAFFPGGGGQ
                     FGDLGKIENDDVINVYEDGKNIYHVLDKKPIKIHKVKCSIDWDRREDGMHQHFGQHVL
                     SGCFFKTFNANTTGFHLGNEYSTVDIEGNLEENKIREIELFANEIIRQNIQVETLLPT
                     KKELKKIWLRRDLPNTDEEIRVIKIGDLDTNACCGVHPKSTGELRMIKIKKWEKHKSS
                     TRIEFLAGKRAVDEVLKRDIYLTKICRYLSCSEEDAINGIVNLNSKVEEALIKKRRLE
                     EVVSTYQVKEMIEKANKVGNISVIKKIYDDEDLKYVNKIANKIAEYENSIALLAVKFN
                     DKINLVFACSKNLKDINMGVLLKDAISLIDGKGGGSKVLAQGGGKNNGNLEATLDYAF
                     MKIEKTL"
     gene            11601..12104
                     /locus_tag="PC700_001450"
     CDS             11601..12104
                     /locus_tag="PC700_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2580765.1"
                     /translation="MEQQKDYIELYENEELIEIREARMDDLDTIAKFNYNLAKETEGK
                     ELDMDVLTKGVKALLLDERKGKYHVYTVFDKVVAQIMYTYEWSDWRNGNFLWIQSVYV
                     DKEYRRKGIFNYLFNYIKNICDKDENIVGMRLYVEKENINAKATYESLNMYECDYNMY
                     EYEVIHS"
     gene            12211..12528
                     /locus_tag="PC700_001451"
     CDS             12211..12528
                     /locus_tag="PC700_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580766.1"
                     /translation="MGVLKGMFYVFLFLDLVSIFCFFFNKGKIASNKIVFNAIGVLTF
                     VLCFMLFSYYPNNNLIGKFIASLFFIFGVAGVMLKEKNFLYARLLLTVVIVFSTLRLF
                     VIQ"
     gene            12686..13645
                     /locus_tag="PC700_001452"
     CDS             12686..13645
                     /locus_tag="PC700_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDF2580767.1"
                     /translation="MIESKKSEKLLLSIIYIIFLVYILMIIRIILFRDVPIYAIFKGT
                     FRNVNLIPFYTIYQFIVDSNLDFMKATVNIIENIGIFIPMGIFLPIVCKNLNKKTIII
                     VIILVSLAFELTQYIFALGSSDIDDVILNSLGGIIGINIYINMNKLFPNDIKRFKAII
                     ATSLVLGVLGLGVISKNYHNLLTFKFRPDRKISKILIEENREIIKDINKDTVDIVGTF
                     ESFKKGIITIKAGSNNKVKRPENLIDSDGNIRIYLNENTKLVSYIITKESKMDIVKYE
                     EFDVKNLDLLRRYDTINVWIDKENQPKDKHGIMASKLLIGLYE"
     gene            13760..14545
                     /locus_tag="PC700_001453"
     CDS             13760..14545
                     /locus_tag="PC700_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HDF2580768.1"
                     /translation="MEIIKYILYFFIYSFLGWTVESIGCSIASKRIINRGFLNGPICP
                     VYGFGAVIVISLLGRFNNVVIVFLLGMILTTILEYFTGFILETLFHAKWWDYSDRKFN
                     IKGRVCLKNAIYFGVMSVLIIRFIHPFIKYFVSIIPYRILISMAIITTLWTILDLIVT
                     IITLKKLDIKLNLLDDIITDLNDINVKLDKFDRGEIQALFKTINNDGLEVREKINKIN
                     SKLDRIESNIILQKRVIKAFPHIKHKKQQEQLEHFKKIINEKR"
     gene            14837..16156
                     /locus_tag="PC700_001454"
     CDS             14837..16156
                     /locus_tag="PC700_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2580769.1"
                     /translation="MSEITSQMISTEEKKYVAKTQKIPYYPVAFKSGDGAMLYDYEGN
                     EYVDFLASAGSANVGHGNKEISQAVKEQMDDITQYTLAYFHSDPPVKLAEKLVEIAPG
                     DNDKKVLYSATGSACIDAAIKLARGYTGRTKIISMCESYHGSTYGAISISALSTNMRR
                     KMGPLLPEVYHFHYPDKNRTAKECLDEIEYAFAHYLPAEEVAAIFIEPIAGDAGIIVP
                     PVEWVQGLSKICKENGILLVSDEIQQGMGRTGKWFGIENFGVEADLIVLGKSVGGGLP
                     LGAVVGRTEIMQSLDAPAHLFTLAGNTTVCVAALKSIEIIEKENLLQKSIEMGDYIKA
                     GFEKLKEKYDIIGEIRGIGLSIGVDIVKGKGSNEKHPDATAKICYRCIQTGLIMIFLG
                     QSTLRVQPPLVITKEQVDKAMSIIDSAIDDYLNGRIGDEVYEVTQGW"
     gene            complement(16231..16659)
                     /locus_tag="PC700_001455"
     CDS             complement(16231..16659)
                     /locus_tag="PC700_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580770.1"
                     /translation="MKDNNNTNKTIQFGEKKASLENNIQKNKNIKLDNTHKVIPYPKN
                     RKKDDKKKGNKKKNTNTSKVNKNNYGHSFFNNKYKYKRKPYVIALYTVILILLAILII
                     TKLTDSTNKGNYSRNTVSDTHKNDIRNIVFNESNNFLICG"
     gene            complement(16750..18126)
                     /gene="hydG"
                     /locus_tag="PC700_001456"
     CDS             complement(16750..18126)
                     /gene="hydG"
                     /locus_tag="PC700_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HDF2580771.1"
                     /translation="MFINHELINSLLEDAKNSTSDDIEKVLDKADRREKLSYKDIATL
                     LEVEDKKQLDRLFSIAGQIKNEIYGNRVVLFAPLYVSNYCVNECVYCGFSKCNKFKRK
                     KLTMEEIKEEVKILEKMGHKRLALEAGEDPKNCDINYILDCLDAIYSTYNENGNIRRV
                     NVNIAATSVDEYKLLKEKGIGTYILFQETYHKPTFIKMHGQSIKNDYYYHLTAFDRAM
                     EAGVDDVGAGVLFGLSDPKFEVLGLMMHNEHLEEKFGVGFHTISFPRLKKAEGMSLED
                     FPHLVSDDMFKKIVAITRLAVPFTGIIMSTRETAEMRNELLKYGVSQISAGSLTGVGG
                     YKAYEDGDNTEQFEVGDHRSPVEVLKELITDGYIPSYCTACYRKGRTGDRFMSLAKSR
                     QIHNVCTPNALTTLNEFLIDYGDEELKIMGKKLIAEEIGKIEREDIRNIVSNNMTALE
                     RGERDLYL"
     gene            complement(18127..19212)
                     /gene="hydE"
                     /locus_tag="PC700_001457"
     CDS             complement(18127..19212)
                     /gene="hydE"
                     /locus_tag="PC700_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HDF2580772.1"
                     /translation="MTKNKIKYFIDKLYSINSLSFDELLYLIENIDNEEYNFEQNLRN
                     YLYKQASDMRNKHYGNRVYLRGLIELTNYCKNNCYYCGIQSSNKNIIRYRLSPDEILE
                     CCDIGYKIGYRTFVLQGGEDAYFTDDKICEIVSSIKNKYEDCAITLSLGEKSYDSYKK
                     YFLCGADRYLLRHETATDSHYKKLHPPNIELRTRKECLKNLKEIGYQVGAGFMVESPF
                     QNNKDLVRDLLYLKELNPHMVGIGPFIPHHDTIFKDYKHGDLEKTLLMLSITRLLLPK
                     VLLPATTALASINPSGRNAGLLAGCNVIMPNLSPQEFRKQYSLYDNKAFTGQEACEYH
                     QSLEENIKSLGLEVDYSRGDNIEWRRL"
     gene            complement(19248..20456)
                     /gene="hydF"
                     /locus_tag="PC700_001458"
     CDS             complement(19248..20456)
                     /gene="hydF"
                     /locus_tag="PC700_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HDF2580773.1"
                     /translation="MSLNSTPQSVRVHIGLFGKRNAGKSSIINAITNQSAAIVSDIAG
                     TTTDPVFRPMEILPIGPCVLIDTAGLDDVGELGELRIGKSLDVLEKTDIALLVVDCQI
                     GISQEDLSLIEKFNDKNIPHILILNKIDTIKNQSEILNLTKNKVKCPVVSVSSTDKIG
                     IENLKNEIIKVLPKDSTEFKLVSDLIEPNDLVVLVVPIDKAAPKGRLILPQQQVIRDI
                     LDNGAISIVTKEDSLKETLSNLGKKPKLVITDSQVFPQVNKDTPKDIPLTSFSILFAR
                     QKGDLKELINGAYALENLKDGDKILMAEGCTHHRQTDDIGTVKIPNMIRKKTGKNITF
                     EFSSGVSFTEDINKYALVVHCGACMMNRAGMLSRIEKAKSFNVPIVNYGILIAYVKGI
                     LERSLELFNY"
     gene            complement(20471..20722)
                     /locus_tag="PC700_001459"
     CDS             complement(20471..20722)
                     /locus_tag="PC700_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HDF2580774.1"
                     /translation="MKKVAVISAILEEPKECQFKFNEVVSGFKGIIKGRMGIPCEEEG
                     VSIVCIAVVGEMDTINSLTGKLGNIPHVSVKTSISKKEV"
BASE COUNT         7887 a         2468 c         3396 g         7206 t
ORIGIN      
        1 aaaatattaa agatacaaaa aaactataga aattgacata tattccagta tttgctaaag
       61 tagctgttgg ggaaaattac aaaaattatt aatgcgcatt agataattta ctttaatttt
      121 tttaggattt agggtaattt ataatataaa acattagaaa ggaaaatata aatgagggac
      181 tttttaaaaa attataagtc atcaattata cttttagcat gtactataat aggggtgata
      241 gtaggattag tacttggtcc aaaagcttca atactaaaac catttggaga tttattcata
      301 aatgctctat taatgatagt agtaccatta gtttttctta gtgtttcttc aggtgttgct
      361 agtataaaaa atatggaacg ttttggaaaa ataatgaaga gtacagctat agtatttatg
      421 ggaacaacac ttgtaattgc atttttaggt attacatctg cattattatt taatccgtct
      481 aaaggattag atgtatcaag tgtaaaaagt ttaatggtgg ctcaagatgc agaaacttta
      541 gaatcaaaag gaacgttatt agaccaagta gtatcaagta ttacagttaa aaattttgga
      601 gatttatttt ccagtagcaa tttactacaa ttagttatat ttgcagtttt agttggaaca
      661 gcaatagcta tgatgggaga taggggaaat acaataagaa agtttttatc agaaggaacg
      721 gaagcaatat taaaagtagt aagtatagtc atgctttatg ctcctatagg gctaggctgt
      781 tatacagcta caataattgg ggaacttgga agcaaaataa ttcaaggata tgtaagggtg
      841 tttgtaattt attcactaac agcagccatg tatttcttat tattttacag tctgtatgct
      901 tttttatctg caggaaaaca agggtttaga caattctgga gaaatgctat aaaaccaata
      961 gcaacggcac tagctacttc ttcaagttca gcatgtatac catcaaattt agaagctagt
     1021 aaaaatatag gagttcctga tgatatatca gaaacagtta tacctcttgg aataaatata
     1081 cataaagatg ggtcagtttt agcagtaata ataaaagtgg catttttatc aggtatttta
     1141 ggcagtggaa tggcaaatcc agcaacgata ggtcaattat ttataactgt atttgttgtt
     1201 ggagtattta tggctcctgt agcaggtggc tgacttattg gtgagatgct cgtggttagc
     1261 atcttcggtt ttccagtgga ggcaattcca ttgttaacag ttataactac tataactgat
     1321 attcctaata ctatattaaa tacaactgga aatacagtat catcaatgtt agtttcaaga
     1381 ttagtagaag gaaaagattg gcttatagat aaaacagcaa ttactacaaa gaaaataagc
     1441 taattgtaca cttaattaat aaagttaata ggtatcttaa ttttaataaa ttagatatct
     1501 attaactttt taagtaaaaa tataaattta agattcaaat atgatattta taatttgaat
     1561 taactaatat agtatatatt gaaataaata tgtttaaata tctagtttat ttttgaaagt
     1621 aaaagatttc aaatttattt ttattaaaag ataacaagcc ttcatctcgc atcttgccaa
     1681 gctctcttga catagaactt ctgtcaacac ataaaaaatc agccatttca tttcttgaaa
     1741 agggaattgt aaactggtgg ctacctgttt gttctatctg ttttgaaaaa tacataagca
     1801 atttctcgcg agttgtttta cgagaaagta attctatttt attgtgaagt aaaatgtttt
     1861 tttcagctat taattgtagc atattttcaa caagcctatt atgaaataca catgcagatg
     1921 aacaaatacc agttacactt ttaaacttta taaataaaac ttcgcaagaa gatgttgtgg
     1981 ttactgtaac attactttta tgtagtctag tacatgcgaa tacctctcca aataaatctc
     2041 caggcaagag ttcagctaca atattcctat tcccttcgat atcttccttt attatatgag
     2101 cacttccttt aagcataatt ccaatagaag taacttcact tccaacagag aatattatac
     2161 tatcttttgg gtaagtctta tctacaacac ctaaacattc caacatttct tcaagctcat
     2221 cacttttaat tccttcaaat agaggaattt ttttaagtag gtctatatgc ttttctaaaa
     2281 attttttcaa ataatcactt ccttgttgca tatgcaaccg ataatttgtt ttcattgtac
     2341 tatactaagt ttataaaagc aaggggatat aaaaatccta ctacaagaaa tttgaaataa
     2401 agaaatatag gtattaaaaa taaatatata agaaatcaat actttaaatt aaaaaaattt
     2461 tatgaggtga tataaatgat gagaaaaata attaaaattg atgagggaaa gtgtaatggt
     2521 tgtggactat gtgttgaagc ttgtcatgaa gaagctatag gaatggtaaa tggaaaagca
     2581 aaacttttaa gagatgacta ttgtgatggg ctgggagatt gtctgccaac ttgtccaaca
     2641 aatgcgattt catttgagta tagagaagca gcagaatatg atgaagctgc agtaaaagca
     2701 aatatggaag caaagaaagc acaaaagaaa actttagcat gtggatgtcc aggttctcag
     2761 tctaagtcaa taaatagaga ggtttcaaat agtacatcta taagcaatga tatagtagag
     2821 ataaaaggtt ctcaacttaa tcaatggcca gtacaaataa aattagtacc aactaatgca
     2881 tcatatttaa agaatgcaag tcttttaata gcagcagact gtacagcgta tgcatatgga
     2941 aattttcata ataaatttat gaaaaataag gttacattaa tcggatgccc taaacttgat
     3001 gaagttgact atgctgaaaa actgacagag attttaaaag aaaatgatat taaaaatata
     3061 gtagtaacac gtatggaagt tccttgctgt ggtggtatag taaatgctgt taagacagct
     3121 ctacaaaata gtggaaaaat gataccatgg caaatagtta ctatatctgt agatggaaaa
     3181 attgtagatg gagaaattta attaagtgaa tcatagaaaa atataataaa ttttaataaa
     3241 taaaaaggga ggaataaaaa atggaaaaaa tgttttgttt tcaatgtgaa caaacagcag
     3301 gaggtaaagg ttgtactata aatggtgttt gtggtaaaaa ggggggtaca gctaacttgc
     3361 aagacgagct tacaggagtg ttgattggtc tttcaagagc aactattgga aataagaata
     3421 gaccaacaag tgaaacagat aaaattatga tagaggggct attcactact ataacgaatg
     3481 taagttttga tgatgaagct ataaaaagac aaattgaaaa aactgaggta gaaaaaagta
     3541 aacttgtacc tagatgttca gattgttcaa caacttgtaa tagaaatgat aactatgata
     3601 tgaaggattt atggaatgat aatgaggata tacgttcatt aaaatcatta atcttatttg
     3661 gattaagagg catggctgcc tatgcatatc atgcaatggt acttggttat acagacaaag
     3721 acgtaaatga atttttctat gaaggattaa tagcagttgg aggagacttg tctattgacg
     3781 aacttttagg tctagttatg aaaactggag aaataaactt aaaatgtatg gaattgcttg
     3841 atagagcaaa tactgaaact tatggaacac cagaaccgac tcaggtatct atgaaaatcg
     3901 agaaagggcc atttattgta gttacaggac atgaccttta tgatttacac aaattattag
     3961 aacaaacgaa agataagggt ataaatatat atactcatgg tgagttatta ccagcacatg
     4021 catatccaaa actaaaagaa tataaacatc taaaaggtaa ttttggtaca gcatggcaaa
     4081 accaacaaaa agaatttgat aatattcctg ctccaatatt atttacaaca aactgtttaa
     4141 tgcctgttaa agatagttat agagatagag tatttactac agaagtagtt gcttatccag
     4201 gtatggttca tattgatgaa gataaagatt tttctagtgt aatagaaaaa gctcttgaac
     4261 ttggaggata tagagaagat gttcatatga ctggtattaa tggaggagat acattaacta
     4321 ctggttttgc aagaggtaca gtttcatctg tacaagataa agtagtagat gctgtaaaga
     4381 gtggagctat aagacacttt ttattggttg gaggttgtga tggtgcaaaa cttggaagaa
     4441 attattacac tgaatttgta aaacaagctc ctaaagatac tgttatattg acacttgcat
     4501 gtggaaaata tagatttaat gatttagacc ttggggatat aggtggctta cctcgtttaa
     4561 tggatatggg acaatgtaat gatgcttata gtgcaattaa agtggcagtt tcattggcaa
     4621 atgcttttga atgtggagta aatgatttgc ctcttagcat ggtactgtca tggtatgagc
     4681 agaaagctgt ttgtatactt cttacactac ttcatttagg tatcaaaaat atctatatag
     4741 ggccttcttt gccagcattc ttatctaaga atgtattaga tatattggtt gataaatttg
     4801 cattgacacc tatatctact cctgaagaag atatgaagaa gattttagga taaataaata
     4861 tatctatact taaagatatt tatagttaaa atatttttaa gtatgtcaca attaagataa
     4921 ggggttaaac ttaaaatttg attaaaaaat caattttgag acgacccttt atttattttt
     4981 tgtaataatt gaaaaataaa cctgctttca gtatattctt ttaagaattt ttgtattaaa
     5041 tttataatac ttagatatat aatatcttat atattttatt ttaagtacta aaggtttgaa
     5101 tgtaacttaa atattaaaag aggaacattg taatatgtaa cttgcataaa atatatatac
     5161 tatagtgaaa ttattttgat taaaatataa ggtgttttat gtgggggtat atgtatatga
     5221 aaagttctaa ttttaatata ttggcatttg atggtggtgg attaaaaggg gcattgagta
     5281 tatccatact tgagaggata gtaaaagagt atccaaatct attaaacaat ataaatatgt
     5341 ttggagggac atctacaggg agtctaattg cactaggact tgcgtatggt gtaagcccta
     5401 aagagataaa agaactttac tcaattgaaa attctaagta tatttttaat aaaagttatg
     5461 cagaaatact cagaccaaag tatgaaaaca aaaacttaaa agaagtttta ttaagtatat
     5521 ttccagagga actagaatta aaagatttaa ataaattagt aatgatacca agtttttata
     5581 taggaaatga ggaaaatgca tggaaaccag tgttttataa caatatgcca aattctttta
     5641 caaagactag caaagttgta gatgtagcaa tggcaagtag tgctgctcca gtattttttc
     5701 caacatataa tcgtcatgtt gatggaggaa taatagcaac tgacccaagt ttagcgtgta
     5761 taattcatgc cattgacagt ggatttaaac ttaagaatac aagacttttt tcaataggga
     5821 ctggatatgt atataatagt ataaaggcag atactactga atggggagct attgactgga
     5881 taattaataa agaacccgat ttaccaatta tatctattac cttagaagga aattctcaaa
     5941 tgagtcagtt attttctcaa aaacttcttg gagataatta ctatagatta aatcctaaga
     6001 tggaaaaaga tgtagctatg gatgattgtg atgcattaga atatctaatg tctttaggag
     6061 aaaattgtga tataaaagat gcttttggat ggataaagaa aaagagcttt tatagaaatt
     6121 agattcaatt tgcaattgta tttttgcaca tgatttcatt gagtaggtaa aaataataaa
     6181 atgcagatta ttaagttact tcttagggaa taaataaagt ataatttatt taaggagtga
     6241 tgaaaatgat aaaacttgct acttttgcag gtggatgttt ttggtgtatg gtaaaacctt
     6301 tcgataagta tgatggagtt attaaagtag tttctggata tacaggtggt catgttgaaa
     6361 accctacata tcaagaggta tgttcaggaa ctactggtca tttagaagca attcaaataa
     6421 catatgatga tgatataata agttatgatg aacttctaaa tatcttctgg aaacagatta
     6481 atcctacaga tgatggtgga caatttggtg ataggggaac tcaatataag actgctatat
     6541 tttatcatga tgaagaacaa aaaaatatag ctttagagtc aagagaacat ctggaaaata
     6601 gtaaaatatt tgatagtaaa atagtgactg atataattcc agcaactaaa ttctacgaag
     6661 cagaagaaaa tcatcaggat tattataaaa aaagaccatt aaactacaat atgtattata
     6721 aagggtctgg aagatataat tttgtaaaaa gtcattggga tggaaacaat ataaataggg
     6781 aagagttaaa aaagacttta actcctatac aatttgaagt cactcaaaat gatatgacag
     6841 aagtaccttt tgaaaatgaa tattatgata ataacaaaga gggaatttat gttgatattg
     6901 taagtggaga agtgttattt tcttcaaaag ataaatttga ttctggatgt gggtggccaa
     6961 gtttttcaaa gccagttata gataaatcta tcattgagag aagtgatttc tcacaaggta
     7021 tgtacagaat tgaagtaaga agtgctaaag gaaattctca tttaggtcat gtatttgatg
     7081 atggtccaaa agaacttgga ggtttaagat attgtataaa ttctgcttct cttaaattta
     7141 taccaagaga aaatatggaa gttttaggat atggagaata tttaaaactt tttgagtaat
     7201 taggaatatt tattataaac ttatctaaaa ggtgaaaata taaaaaaata ttttcacctt
     7261 ttgttttttt gtcaataata tgataaatta ttaattgtaa tcttatttat accaatagat
     7321 taagaattta attttgaaag agagaaaaca aatgaattta aaagacttta atggaaagag
     7381 attaaaatca gctagaattt ttagagcaaa gacgatagaa cagttgtcta aagaaactaa
     7441 aataaataaa aaagatttaa aagcttttga ggaaaataag tatgtaccaa atatagaaaa
     7501 tacattaaaa ttatctaata tattaaattt tccaaaagag tatttttata aaaatgaaaa
     7561 tataaatatc gttgtagagg atagccattt taaccctcaa tctaaactac ctagagtaga
     7621 agagatttct tatagagaaa aaataattat aatacataaa atatattcat ttatggagaa
     7681 ttatattaag tttccaaaag aaaacttgcc aaatagaaaa gtaatgtcag atattaatat
     7741 aagtgatata gaatctcttg catataagac tagagagttt tgggatttaa atagtactcc
     7801 aatagtaaat atggtaagtt tgttagaatc taaagggatt ataatatcag gtatgaatgt
     7861 agatagaaaa ggggcaacta tctttactca aaaacagaga attagtaaag aatctaaata
     7921 tctaatatct ctgggtaatg acaaaaagtc agcatcaatt agaaattata ctttggcatg
     7981 tgagcttgga tatataatat caagtgaatt aagaatacca agtaagcaat tttcagaaga
     8041 tgagtatgcc tgtgcatttt tattgccaaa agagagtttt ttaaaagatt tgactcatcc
     8101 agaagattta gattattatg tagaactaaa gaaaaaatgg attgtaccta tatctgctat
     8161 gatttttaga gcatataatc tagaaaagat aaattacaaa aaatacaatt atcttatgaa
     8221 tgaaatggat aaaaaaggct ggttgataga agaaccacta gacaaaatga aaggttcaag
     8281 cccaacgtat ttaaaaagag cagttgaatt attgatagaa aataaaataa tgagtgtaaa
     8341 tagtatagta tcaagtttag aggaatttgg aataaatctt tatccagaag acttagaatt
     8401 attgatggga ttgaaaaaag gattgttatc tcaagaggta aataaaaaaa gtaaagttat
     8461 aaaatttgat ggtaaaaaat aggtttatat tttagtatta aaattaaaag caggtataaa
     8521 tgatttaaac attaacttag ttaaaagtct tttcaataag tcatttacct gcttttagtg
     8581 taagaaagaa tattgaatct aaagtgtttt tgaaagtgca atacattctc ttaaaacaga
     8641 gcaagattta ttaaattcgt tttgctcata ttcagtcata ttgatttcaa taatttcttc
     8701 aataccatct tttcctataa cacatggaac acctgcaaat acatttcttt cgttatattg
     8761 accttctagt aatgtagaaa ctggaagaac ttttttctca ttgtgtagta tagcttttac
     8821 aatttctact aaagcagtac ctataccaaa ttctgtacaa tctttaccat ttattatact
     8881 tatccctgtt tttttatttt tttcaactat tgatggtaag tctaaattgc tataagtatt
     8941 tgggttgtct tttatcatgt ccaagattgg tttaccacca attgaaacat gagaccaagg
     9001 aaccatttga gaatctccat gttcacccat agaataagct tgaattgatt gttgagcgat
     9061 tccagtttct tcagataaaa tccttctaag ttttgttgaa tctaaagctg ttccagttcc
     9121 aatgacttta tttttaggaa agccagattt ttcccaaata taatttgtaa ctacatcaac
     9181 aggattagat atgactacaa atataccatc aaagccagaa tcaactatgg gttttactat
     9241 agattttatc attccaatag ttggtcttag taaatctaat ctagttgtgt tttgtctacg
     9301 taaaggccct tctgaagagt ctgcaacact tatcactact atgtcagaat ctttacagtc
     9361 agaaaaagtt cctttttcta tatgtacttt atgtggtaga tatgaaactg catcagctaa
     9421 atctaaagct tgagcttttg atttagattc atctatatca atcataaaga gttcatcaca
     9481 aactccttga gttattaaac taaatccaca atgagaacca acatgccctg aacctacaat
     9541 agatattttt cttggcttta ttgccatttt aaatcactcc ccctaaaata acgatacatt
     9601 gaaaattata gatgttatca aaagctgttt ttggatattt taatatttta ttatttcaga
     9661 aaatttattt aatatgattt tatactaaaa tttttaaaaa agcaatgtta atatactctc
     9721 tattaatgaa tgttaaagta ttatatgtat attaataatt attaaaatat gtaatgtcac
     9781 tgatagttag aatatttaag tgttaaaaga tatatataaa taataatgat atcagttgat
     9841 aatgatatta aattgaggta tactgtttct ctaaattaaa aaacaaagga gtaaatgtgt
     9901 tatgaattat catacaaaag aagagttgat ggaagtactg agagtagcat cttcaaggat
     9961 tattaattgt gaaaaagtac agaagaaatt ttctgaggag acatctcatc atacacgttt
    10021 taagaatatt atagaagcaa tgtatatttc aaaatcatta ataatggatg aaattagtaa
    10081 aagagattga agcttatttt gcaatttaat atgttttaat atacatttta ttatataaga
    10141 aaataagtaa gcatacttgt tataaaatat tgaaataata taaaattata aatttgttaa
    10201 cactaatatc tatatgttaa aatatagtag aagatgttag taaataatag gatgagttct
    10261 attttaatac aattaaatta agcttagctt gaaatttgta tttaaaaagt aaagtagtct
    10321 ataattaaga aaatggagat agaattataa tggaaaagtt atattataca aatcagtata
    10381 taagagattt tacagcagaa ataattgaaa taaaagaagt agaaaataag tatcatgttt
    10441 tattagataa gacagctttt tttcctggtg gaggaggtca gtttggagac ctgggaaaaa
    10501 tagagaatga tgatgtaata aatgtgtatg aagatggaaa aaatatatac catgtattag
    10561 ataaaaaacc tataaagata cataaggtaa agtgtagtat tgattgggat agaagagaag
    10621 atggaatgca tcaacatttt ggacaacatg tattatctgg atgttttttt aaaactttta
    10681 atgcaaatac gacaggtttt catttaggaa atgagtatag tacagtagat atagaaggta
    10741 atttagaaga gaacaaaatt agagagatag agttgtttgc caatgaaatt attcgccaaa
    10801 atatacaagt agagacatta cttccaacga aaaaagaact aaaaaagatt tggttaagac
    10861 gtgatttgcc aaatactgat gaagagataa gagtaataaa aataggtgat ttagatacga
    10921 atgcatgttg tggagtgcat ccaaaatcaa ctggtgaatt gagaatgata aaaataaaga
    10981 aatgggaaaa acataagtca agtacaagaa tagagtttct agcaggtaaa agggcagtgg
    11041 atgaagtatt gaagagggat atatatttga ctaaaatatg tagatattta agctgtagtg
    11101 aggaagatgc tataaatggt attgtaaatc taaatagtaa agtggaagag gctttaatta
    11161 aaaaaagaag gcttgaagaa gtagtatcta catatcaggt caaagaaatg attgaaaaag
    11221 caaataaggt tggcaatatt tcggtcatta aaaaaattta tgatgatgaa gatttgaaat
    11281 atgtaaataa gatagcaaat aaaattgcag aatatgaaaa tagtattgca ttattagctg
    11341 ttaaatttaa tgataaaata aatttagttt ttgcatgttc taaaaattta aaagatatta
    11401 atatgggagt tttattaaag gatgcaataa gtcttataga tggcaagggt ggtggaagta
    11461 aagtattagc tcaaggagga ggtaagaata atggaaattt agaggctaca cttgattatg
    11521 catttatgaa gatagaaaaa actctataaa tatattatat aaaaaacctt agtagataaa
    11581 atagatatgg aggaataagt atggagcaac aaaaggacta catagagtta tatgaaaatg
    11641 aagaactaat agagataaga gaagcacgta tggatgattt ggatacaata gcaaaattta
    11701 attataattt ggctaaagaa actgaaggaa aagaacttga tatggatgtt ttaactaaag
    11761 gtgtaaaagc attgttatta gatgagagaa aaggaaaata tcatgtatat acagtatttg
    11821 ataaagttgt tgcacagatt atgtatacat atgagtggag tgattggaga aatggaaatt
    11881 tcctttggat tcagagtgta tatgtagata aggaatatag aagaaaaggt atctttaatt
    11941 atttatttaa ttatataaaa aatatatgtg ataaagatga aaatatagtt ggaatgagat
    12001 tatatgtaga aaaagaaaac ataaatgcaa aagcaacata tgagtcttta aatatgtatg
    12061 agtgtgatta taatatgtat gaatacgaag taatacattc gtaatataaa tttgtaatac
    12121 tgttatactg atgttataaa gtattgatta tattgacgat tatatgaaat aaactggata
    12181 ttataattag aatttttagg gggtataaaa atgggcgttt taaaaggaat gttttatgta
    12241 tttttatttt tagatttagt gtcaatattc tgtttctttt tcaataaagg aaaaatagca
    12301 tctaataaaa ttgtgtttaa tgcaatagga gtacttacat ttgtactttg ttttatgctt
    12361 tttagttatt atcctaataa taatcttatt ggaaaattta ttgcttctct attttttata
    12421 tttggagttg caggagttat gttgaaagaa aaaaattttt tatatgctag acttctttta
    12481 actgtagtta tagttttttc tacattaaga ttgttcgtta ttcaataaaa tgtatttgta
    12541 tgaaaattta ttaaagtcta cacatttaag tgtaggcttt tttgattttt tgtttgaaaa
    12601 ttaaggaaat tttaaggaaa ttttaaaatc gtcttaaata tttattgata tacttaaaat
    12661 attaatagcg aaaggagact tatcagtgat agaaagtaaa aagtcagaaa aattgttact
    12721 gtcaattata tacataatat ttttggtata tattttaatg attataagaa taattttatt
    12781 tagagatgta ccaatttacg ctattttcaa gggaacgttt aggaatgtaa atttaattcc
    12841 attttataca atatatcaat ttatagtaga tagtaatctt gactttatga aagctactgt
    12901 aaatattata gaaaatatag gtatatttat tcctatggga atttttttac ctatagtatg
    12961 taaaaatcta aataaaaaaa ctataattat agtaattata cttgttagcc tagcatttga
    13021 attaacacaa tatatttttg ctcttggaag tagtgatata gatgatgtta ttttaaattc
    13081 attaggtgga ataattggaa ttaatattta cataaacatg aataaattat ttcctaatga
    13141 tattaaaaga tttaaggcta taatagctac tagtttagtt ttaggagttt taggattagg
    13201 agttatttct aaaaattatc ataacttact aacttttaaa tttagacctg atagaaaaat
    13261 atcaaaaata ttaattgaag aaaataggga aataattaaa gatataaata aagatacagt
    13321 agatatagtt gggacatttg agagctttaa aaaaggaata ataactatta aagcaggttc
    13381 taataacaaa gtaaagagac cagagaattt aattgattct gatggaaata taagaattta
    13441 tttaaatgaa aatacaaaat tagtatctta tataattacc aaagaatcaa aaatggatat
    13501 agttaaatat gaggaatttg atgtgaagaa tttagattta ttaaggagat atgatactat
    13561 taatgtttgg attgataaag aaaatcagcc aaaggataaa catggtatta tggctagtaa
    13621 attattaatt ggattgtatg aataaaatat taaacaagaa taaaagaata acaaatataa
    13681 aaaacgaaaa aactatttct ataattattt ttacgttgtg cataaaataa gaacattagt
    13741 gaaggagtag agataaatta tggagataat aaaatatatc ttgtactttt ttatatattc
    13801 atttttagga tggacagtag aaagtatagg atgttctata gcaagcaaaa gaataatcaa
    13861 cagaggtttt ttaaatggtc caatatgccc tgtttatggt tttggagcag taattgtgat
    13921 atcactatta ggaagattta ataatgtagt aatagtattt ctattaggaa tgattttaac
    13981 tacgatacta gaatatttta caggatttat tttagaaacc ttatttcatg ctaaatggtg
    14041 ggattattct gacagaaaat tcaatataaa gggtagagta tgtttaaaaa atgcaatata
    14101 ctttggtgta atgtctgttt taataataag atttatacat ccatttataa aatattttgt
    14161 aagtataatt ccatatagaa tattaatttc aatggctata attacaacac tttggactat
    14221 acttgatttg attgttacta ttataacact taaaaaatta gatataaagt taaacttact
    14281 agacgatata attacagacc ttaatgatat aaatgtgaaa ttggataaat ttgatagagg
    14341 agaaatacaa gctttattta aaacaataaa taacgacgga ttagaagtta gagaaaaaat
    14401 aaacaaaata aatagtaagt tggaccgtat agagtctaat attattttac aaaaaagagt
    14461 tataaaagca tttccacata taaaacataa aaaacaacaa gagcaactag aacattttaa
    14521 aaaaattatt aatgagaaaa ggtaattata caatttaatg aagacgaata gaataatggt
    14581 atttataaga ttattttcag aggactctat aaaaatagaa tagagtcctt tattttttga
    14641 aaaataatgt ttatttgata aaaataaaat atattgtttc taaataacaa atattttgaa
    14701 aattataaaa acatatattg acaatttgtg tcaaaaatac taacattaag tatataaaga
    14761 attttatatg agaatatctt tataaatcgc aataatttta gaacataaat ttatttaaag
    14821 aggggatgat ttgccaatga gcgagataac tagtcaaatg ataagtacag aagaaaaaaa
    14881 atatgttgct aaaactcaaa agatacctta ttatccagta gcatttaaat caggtgatgg
    14941 tgcaatgcta tatgattatg aaggtaatga atatgtagat tttttagcaa gtgcaggctc
    15001 tgcaaatgta ggtcatggta ataaggaaat ctcacaagca gttaaagaac aaatggatga
    15061 tataacacaa tatacacttg catatttcca tagtgatcct cctgtaaaat tagctgagaa
    15121 gcttgtggaa atagcccctg gagataatga taaaaaagta ctatacagtg caactggttc
    15181 tgcttgtata gatgctgcta taaaattagc aagagggtat actggaagaa caaagataat
    15241 atctatgtgt gaatcttatc atggaagtac ttatggagct atatctatat ctgctttaag
    15301 tacaaatatg agaagaaaaa tgggtccatt gttaccagaa gtttatcact tccattatcc
    15361 tgataaaaat agaacagcta aagaatgttt agatgagata gaatatgctt ttgcacacta
    15421 tctaccagca gaagaggtag ctgcaatatt tatagagcca attgcaggag atgcaggaat
    15481 aatagtgcct ccagtagagt gggtacaagg tttaagtaaa atatgtaaag aaaatggaat
    15541 attacttgta agtgatgaaa tacaacaagg tatgggaaga acaggtaagt ggtttggtat
    15601 agaaaatttt ggtgttgaag cagaccttat agttcttgga aaatctgttg gaggtggatt
    15661 acctttagga gcagttgttg gaagaacaga aataatgcaa agtcttgatg caccagcaca
    15721 tttatttact ttagctggaa atacaacagt gtgtgtcgct gctttaaaat ctatagagat
    15781 aattgaaaaa gaaaacttac ttcaaaagag tatagaaatg ggagattata ttaaagcagg
    15841 atttgaaaaa ctaaaagaaa aatacgatat aattggagaa ataagaggaa tagggttatc
    15901 tataggagtt gatatagtta aaggaaaagg ttctaatgaa aaacatccag atgctacagc
    15961 taagatatgt tatagatgta tacaaactgg attaataatg atattcttag gacaatctac
    16021 tttaagagta caaccacctc tagtaataac taaggagcaa gtagataaag caatgagcat
    16081 catagattct gcaatagatg attatttaaa tggtagaatt ggtgatgaag tatatgaagt
    16141 aactcaaggt tggtaaaata aatattaaat gaaaaaaaga gttatctcaa ttttagaaag
    16201 taatttctga aatgagataa ctcttttaag ttacccacaa ataagaaaat tgtttgattc
    16261 attaaagact atatttcgga tgtcgttttt gtgagtatca gagacagtat tacgagaata
    16321 attaccttta ttcgtactat ctgttaattt agttataatt aagatagcta aaagaataag
    16381 aattactgtg tataaagcaa taacatatgg ttttctttta tatttgtatt tgttattgaa
    16441 aaaagaatgg ccataattat ttttatttac tttagatgta tttgtattct ttttcttgtt
    16501 acctttcttt ttatcatcct tttttctatt tttagggtaa ggaatgactt tgtgtgtatt
    16561 atcaagtttg atatttttat ttttttgaat attgttttcc aggctagcct ttttttctcc
    16621 aaattgaata gttttattgg tattgttgtt atctttcaat gttaatctcc ctcccttaaa
    16681 ctttcccttt agataatatt actactaacc tatggaatat acaataaata atatataagt
    16741 aataaaattt tataggtata aatctctttc tccacgctct aaggcagtca tattgttaga
    16801 tactatgttt cttatatctt ctctttctat cttacctatt tcttctgcga taagtttttt
    16861 acccataatt ttaagttctt catctccata atcgattaaa aattcattta atgtagtaag
    16921 agcatttggc gtacatacat tatggatttg tcgactttta gctaagctca tgaatctatc
    16981 gccagttcta cctttacgat aacaagctgt acaataacta ggtatatatc catcagtaat
    17041 cagttcttta agcacctcaa cagggcttct atggtctcca acttcgaatt gttctgtatt
    17101 gtcaccatct tcatatgctt tatatcctcc aacacctgtt agagaacctg cactaatttg
    17161 tgatacacca tattttagta actcatttct catttcagca gtttctctag tggacattat
    17221 gattccagta aaaggaacag ccaatctagt tatagctact atttttttga acatatcgtc
    17281 agaaactaag tgaggaaaat cttctaagct cataccttca gcttttttca atctagggaa
    17341 agatatagta tggaaaccta caccaaattt ttcctctaag tgttcgttgt gcatcataag
    17401 tcctagaact tcaaatttag ggtcagataa accaaataaa actcctgcac caacatcatc
    17461 aacaccagct tccatagctc tatcaaaagc tgttaaatgg taataatagt cattttttat
    17521 agattgacca tgcattttta taaatgttgg tttgtgataa gtttcttgga ataatatata
    17581 agttcctatt cctttttctt ttagtaattt atactcatct acacttgtag ctgctatgtt
    17641 aacgtttacc cttcttatat ttccgttttc attatatgtt gaataaattg catctaagca
    17701 atctaagatg taatttatat cacaattttt agggtcttca ccagcttcta gagcaagtct
    17761 tttatgaccc attttttcta atattttaac ctcttccttt atctcttcca tggtaagttt
    17821 ttttctttta aatttgttgc atttactaaa accgcaataa acacattcat ttacacagta
    17881 gtttgataca taaagagggg caaataaaac cactctatta ccatatattt cattttttat
    17941 ttgcccagct atactaaata atctatctag ttgtttttta tcttctactt caagaagtgt
    18001 agctatatct ttataactaa gtttttccct tctatcagcc ttatcaagta ccttttcaat
    18061 atcatcagaa gttgaatttt ttgcatcttc taataaagaa ttaataagtt catggtttat
    18121 gaacatttac aaacgcctcc attcaatatt atcaccgcga gaatagtcaa cttcaagtcc
    18181 taatgacttg atgttttctt ctaaactttg gtgatattca caagcttctt gaccagtaaa
    18241 ggctttgtta tcgtaaagag aatattgttt tctaaattct tgaggagaaa ggtttggcat
    18301 aattacattg caaccagcta aaagacctgc atttctacca ctagggttta tagatgccaa
    18361 ggctgttgta gcgggtaata aaacttttgg aagtagaagt cttgtaattg aaagcataag
    18421 taatgtcttc tctaaatctc catgtttata atccttaaaa attgtatcat ggtgtggaat
    18481 aaaaggtcct attccaacca tatgaggatt taactctttt aggtataata aatcacgaac
    18541 aaggtctttg ttgttctgaa atggtgattc aaccataaat ccagcaccta cctgataacc
    18601 aatttcttta aggtttttta ggcattcttt tctagttcta agctcaatgt ttggcggatg
    18661 taatttttta taatgactgt ctgtagcagt ttcatgtctt aaaagatatc tatctgcacc
    18721 acataaaaag tattttttat acgaatcata actcttttct cctagtgaca gagttatggc
    18781 acaatcctca tatttatttt ttatacttga aacaatttca catattttat catcagtaaa
    18841 atatgcatct tcaccacctt gcaaaacgaa tgttctgtat cctattttat aacctatatc
    18901 acaacattca agtatttcat caggtgaaag tctgtatctt atgatatttt tattagaaga
    18961 ttgtattcca caatagtagc aattattttt acaatagttt gtaagctcta taagtcctct
    19021 caagtagact ctatttccat aatgtttatt tctcatgtcg ctagcttgtt tataaagata
    19081 gtttcttaaa ttttgttcga agttatattc ctcattgtcg atattttcta ttaaatatag
    19141 tagttcatca aaacttaatg aattaatgct gtaaagttta tctataaagt atttaatctt
    19201 atttttagtc atattcaaat atgtaattat taagtatagt taaattttta ataattgaaa
    19261 agttcaagtg atctttccaa aatacctttt acatatgcta ttaagatacc atagtttaca
    19321 ataggtacat tgaaagattt tgccttttct attctagata acattccagc tctattcatc
    19381 atacaagctc cacagtgaac tacaagggca tatttattta tatcttcagt gaaggatact
    19441 ccagaactaa attcaaaagt aatatttttg ccagttttct ttctaatcat attaggaatt
    19501 tttactgttc caatatcatc agtttgtcta tgatgggtac atccttcagc catcaagatt
    19561 ttatcaccat cttttaaatt ttctaaagca taagctccat taatcaattc tttaaggtct
    19621 cccttttgtc tagcaaacaa tatagaaaaa gatgtaagag gaatatcttt tggtgtatct
    19681 ttatttactt gtggaaatac ttgagagtca gtaataacaa gttttggttt tttgcctaaa
    19741 ttagatagag tttcttttaa actatcttct tttgtaacta tagaaattgc accattatcc
    19801 aaaatatctc ttataacttg ctgttgagga agtataagtc taccttttgg agctgcttta
    19861 tctattggaa caactaaaac aactaaatca tttggttcaa ttaaatctga taccaatttg
    19921 aactcagtgc tatccttagg caaaactttt attatttcat tttttaaatt ttcaattcca
    19981 attttatctg ttgatgagac agaaacaact ggacatttaa ctttattttt agttaagttt
    20041 aaaatttctg attgattctt aatagtatca attttgttga gaattagaat atgagggata
    20101 ttcttatcat taaacttttc aattagtgac aaatcttctt gtgatatacc aatttggcaa
    20161 tccactacta gtaaagctat atctgtcttt tctaaaacat ctagtgattt acctattcta
    20221 agttcaccaa gttcaccaac atcatcaaga ccagcagtat ctataagaac acaaggtcct
    20281 ataggaagga tttccattgg tctaaatact gggtctgtag tagttccagc aatatctgag
    20341 actattgcag cactttgatt tgttatagca tttattatgc tagatttacc tgcatttctt
    20401 ttaccaaaga gacctatatg tactcttacc gattgtggag tagaatttag actcatttgt
    20461 actcaccacc ttaaacttct ttttttgata tactagtttt tacagataca tgaggtatgt
    20521 tgcccaattt accagtcaaa ctattaatag tatccatttc gccaacaact gctatacata
    20581 cgattgagac accttcttct tcgcaaggaa tgcccattct tcctttaata atacctttaa
    20641 aacctgatac aacctcattg aatttaaatt gacattcttt tggctcttcc aaaatagcac
    20701 ttataactgc aacctttttc ataaatatcc tcctaactca gaaaatatgt cttaaacaaa
    20761 tccaaaatca aaaaactttt ctttgtaaag aaaagtttct acaaaagaaa agttattaat
    20821 tacacaagta taatgtgcta tattaaacta tttccttaca gattagataa ctcttttctg
    20881 gtagggttag cttgtaaaaa tttaatttaa caacaagtaa ctgaagaaaa tttttataca
    20941 atttatataa attataa
//
LOCUS       DAMJWP010000046        20707 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.047, whole genome shotgun sequence.
ACCESSION   DAMJWP010000046 DAMJWP010000000
VERSION     DAMJWP010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20707
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.047"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            358..669
                     /gene="rpsJ"
                     /locus_tag="PC700_001460"
     CDS             358..669
                     /gene="rpsJ"
                     /locus_tag="PC700_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDF2580775.1"
                     /translation="MAKNEKIRIRLKSYDHKLLDFSAGKIVETAKKAGSQVSGPVPLP
                     TEKQVVTILRAVHKYKDSREQFEIRTHKRLIDIANPTPKTVDSLMRLDLPAGVDIEIK
                     L"
     gene            761..1390
                     /gene="rplC"
                     /locus_tag="PC700_001461"
     CDS             761..1390
                     /gene="rplC"
                     /locus_tag="PC700_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDF2580776.1"
                     /translation="MKGILGKKVGMTQIFTDKGVVIPVTAVEAGPMVVTQIKTVDKDG
                     YNAIQIGFEDAKEKALNKPKKGHLAAANVLKKHLKEFRVDSVEGYTVGQEIKADVFEA
                     GAKIDVTGISKGKGFQGPIKRHGQSRGPETHGSRYHRRPGSMGACSYPGRVFKNKKLA
                     GHMGSVKVTVQNLEVVKVDADKNLILVKGAIPGAKGSVVTIKEAIKVSK"
     gene            1420..2043
                     /gene="rplD"
                     /locus_tag="PC700_001462"
     CDS             1420..2043
                     /gene="rplD"
                     /locus_tag="PC700_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDF2580777.1"
                     /translation="MPKLNVLNVSGQNVGEIELSDSIFGVEVNGHVLYEVVKNQLANK
                     RQGTQSAKTRAEVRGGGRKPWKQKGTGRARQGSTRSVQWVGGGVAFAPKPRSYKYTLP
                     KKVRRLAMKSALSSKVQNSEVIVLDALNMDAPKTKEFAQILNNINAAKKALVVIADKN
                     DNVIKSARNIEGVQTALVNTMNVYDILKYDSFIITTDAVKKVEEVYA"
     gene            2043..2333
                     /gene="rplW"
                     /locus_tag="PC700_001463"
     CDS             2043..2333
                     /gene="rplW"
                     /locus_tag="PC700_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDF2580778.1"
                     /translation="MTNPHDVIIRPVVTEHSMAEMGEKKYTFVVAKDANKTEIKKAVE
                     KVFGVSVDKVNTLNYDGKVKRMGRTQGRTASFKKAVVKLTADSKEIEFFQGM"
     gene            2363..3193
                     /gene="rplB"
                     /locus_tag="PC700_001464"
     CDS             2363..3193
                     /gene="rplB"
                     /locus_tag="PC700_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDF2580779.1"
                     /translation="MAIKKFRPTSPALRQMTVLVSDEITCNQPEKSLLVNLKKNAGRN
                     VHGRITVRHRGGGQKRKYRIIDFKRDKDGIPAKVATIEYDPNRTANIALLNYADGEKR
                     YILAPVGINVGDTILSGLGADIKPGNCLALKDMPVGTIIHNIELKPGKGAQLVRSAGV
                     SAQLMAKEGKNALLRLPSGEMRLVSINCKATIGQVGNIEHGNVVIGKAGRKRHMGIRP
                     TVRGSVMNPNDHPHGGGEGRSPIGRPSPVTPWGKPALGYKTRKKNKASNKLIVSRRTK
                     "
     gene            3228..3509
                     /gene="rpsS"
                     /locus_tag="PC700_001465"
     CDS             3228..3509
                     /gene="rpsS"
                     /locus_tag="PC700_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDF2580780.1"
                     /translation="MSRSTKKGPFVHARLLKKIEAMNASGNKEVIKTWSRSSTVFPQM
                     VENTIAVHDGRKHVPVYITEDMVGHKLGEFVPTRTFKGHKDDEKSNKRK"
     gene            3541..3876
                     /gene="rplV"
                     /locus_tag="PC700_001466"
     CDS             3541..3876
                     /gene="rplV"
                     /locus_tag="PC700_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDF2580781.1"
                     /translation="MEAKATAKYVRVSPRKAGQICDLVRGKNVDEALAILKFTPRGAA
                     SIIAKVVKSAKANAENNHEMDTEKLYIASIVANQGPTMKRFMPRAMGRATTIRKRTSH
                     IEVVVKEKK"
     gene            3899..4714
                     /gene="rpsC"
                     /locus_tag="PC700_001467"
     CDS             3899..4714
                     /gene="rpsC"
                     /locus_tag="PC700_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDF2580782.1"
                     /translation="MGQKVNPHGLRVGVIKDWDSRWFATDKKEFGNLLLEDHNIRKFL
                     KKRLYSAGVAKIEIERSANKIKMDLHVAKPGVVIGRAGAGIEALKAELEKMTKKTIIV
                     NIVEVRSTDKNAQLVAENIALAIERRVAFRRAMKQAIQRAMKSGAKGIKVSASGRLGG
                     AEMARTEGYSEGNVPLQTLRADIDYGFAEADTTYGKIGIKVWICNGEVLPTRDGVNPR
                     EESRKSDRRDNKRDNRRNDRRGNDRRGNDNRGNYRGQRPQGGSRPQRTENKGN"
     gene            4750..5181
                     /gene="rplP"
                     /locus_tag="PC700_001468"
     CDS             4750..5181
                     /gene="rplP"
                     /locus_tag="PC700_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDF2580783.1"
                     /translation="MLMPKRVKRRRVHRGSMAGQAHKGNKVTYGEFGLVALEASWITS
                     NQIEAARIAMTRYIKRGGKVWIKIFPHKPVTRKPAETRMGAGKGSPEYWVAVVKPGRV
                     MFELAGVSEDKAREAMRLAAHKLPIKCKFVKKEDLEVKGGE"
     gene            5183..5386
                     /gene="rpmC"
                     /locus_tag="PC700_001469"
     CDS             5183..5386
                     /gene="rpmC"
                     /locus_tag="PC700_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDF2580784.1"
                     /translation="MKAKELRDLTSEELMNKLNDFKSELFSLRFQLATGQLENTARIK
                     FVKKDIAKVKTVLAERKLYETRA"
     gene            5410..5664
                     /gene="rpsQ"
                     /locus_tag="PC700_001470"
     CDS             5410..5664
                     /gene="rpsQ"
                     /locus_tag="PC700_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDF2580785.1"
                     /translation="MERGRRKVRIGRVVSDKMDKTIVVAVEEFVRHPLYNKRVKRTKK
                     FKAHDEKNICNIGDRVKIMETRPLSKDKRFRLVEVVEKVK"
     gene            5690..6058
                     /gene="rplN"
                     /locus_tag="PC700_001471"
     CDS             5690..6058
                     /gene="rplN"
                     /locus_tag="PC700_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDF2580786.1"
                     /translation="MIQQESRLRVADNSGAKELLCIRVLGGSKRRYGNIGDVIVATVK
                     SATPGGVVKKGKVVKAVIVRSKQGVRRNDGSYISFDENAAVIIKDDKTPVGTRIFGPV
                     ARELRDNEFMKIVSLAPEVL"
     gene            6082..6387
                     /gene="rplX"
                     /locus_tag="PC700_001472"
     CDS             6082..6387
                     /gene="rplX"
                     /locus_tag="PC700_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDF2580787.1"
                     /translation="MRVKKGDTVVVIAGKDKGKKGSVLKVYPKTSKVLVEGVNVITKH
                     QKPSAMNQQGGIINKEAPIHISNVMPFDPETGKGVRVRYEVKDGNKVRVSAKSGKEL"
     gene            6417..6959
                     /gene="rplE"
                     /locus_tag="PC700_001473"
     CDS             6417..6959
                     /gene="rplE"
                     /locus_tag="PC700_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDF2580788.1"
                     /translation="MASRLQEKYMKEVAPALMEKFGYKNVMEIPKLNKIVINMGIGDA
                     RENPKGLEKGVEELEMISGQKPVITKARKSVANFKLREGMPIGTKVTLRADKMFYFMD
                     KLVSVSLPRVRDFRGVNPNAFDGRGNYALGVKEQLIFPEIEYDKIDKVRGMDIIFVTT
                     AKTDEEARELLKLLGMPFSK"
     gene            6977..7162
                     /locus_tag="PC700_001474"
     CDS             6977..7162
                     /locus_tag="PC700_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HDF2580789.1"
                     /translation="MARKAMVVKQQRKQKYATREYTRCTICGRPHSVLKKFGICRICF
                     RELAYKGQIPGVRKASW"
     gene            7195..7593
                     /gene="rpsH"
                     /locus_tag="PC700_001475"
     CDS             7195..7593
                     /gene="rpsH"
                     /locus_tag="PC700_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDF2580790.1"
                     /translation="MTMTDPIADMLTRIRNANVVKHETVDVPASNMKKELARILLEEG
                     FIRGYDVIEDGKQGIIRIQLKYGQEGERVITGLKKISKPGMRVYAANHEIPKVLNGLG
                     ISVISTSKGILTDKQARKENVGGEVICYVW"
     gene            7624..8166
                     /gene="rplF"
                     /locus_tag="PC700_001476"
     CDS             7624..8166
                     /gene="rplF"
                     /locus_tag="PC700_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDF2580791.1"
                     /translation="MSRIGVKPIIIPAGVEVTIAEGNLVTVKGPKGTLTKQLSAELNI
                     KKEENTIMVERPTDNKKHRSLHGLTRTLLDNMVVGVNTGFEKKLELKGVGYRAQKQGK
                     KLVMNLGFSHPVEMEDPEGITVEAPNQTELIVKGIDKQLVGNYAAKIRAWRKPEPYKG
                     KGIKYVDEVIRRKEGKTGKK"
     gene            8202..8570
                     /gene="rplR"
                     /locus_tag="PC700_001477"
     CDS             8202..8570
                     /gene="rplR"
                     /locus_tag="PC700_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDF2580792.1"
                     /translation="MLKKADKNANRLQRHKRVRRKISGTSQRPRLCVFRSANNIYAQI
                     IDDTKRVTLVAASSLEAEVKSAVNHTGNKEAAKKVGELVAKKAVEKGITEVVFDRGGY
                     LYHGRIQELAEGAREAGLKF"
     gene            8591..9100
                     /gene="rpsE"
                     /locus_tag="PC700_001478"
     CDS             8591..9100
                     /gene="rpsE"
                     /locus_tag="PC700_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDF2580793.1"
                     /translation="MLRRKPIDAGQLDLQEKVVEVRRVTKVVKGGRNFRFAALVVVGD
                     ENGHVGIGAGKAMEVPDAIKKAVEDAKKNLIVVPIVGTTIPHEVRGHFGAGNILIMPA
                     VEGTGVIAGGPARAVLELAGLKDVRAKSLGSNNPRNMVNATIEGLNSLKTVEDIAKLR
                     GKKVEELLG"
     gene            9115..9300
                     /gene="rpmD"
                     /locus_tag="PC700_001479"
     CDS             9115..9300
                     /gene="rpmD"
                     /locus_tag="PC700_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDF2580794.1"
                     /translation="MAKLQIKLVRSVIGTTPNQKKNVEALGLRKREQVVVKEDNAQTR
                     GMINKVSHLLEVTEIAE"
     gene            9333..9776
                     /gene="rplO"
                     /locus_tag="PC700_001480"
     CDS             9333..9776
                     /gene="rplO"
                     /locus_tag="PC700_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDF2580795.1"
                     /translation="MKLHELKPAEGAVRAKRRLGRGTATGQGKTAGRGQKGQWSRSGG
                     GVRVGFEGGQMPLARRLPKRGFNNIFKKVYTEVNVEVLNRFENGTEITAELLKSTKTI
                     SKIGKDGIKILGEGNLEKALTVKAAKFTASAQEKIEKAGGKAELV"
     gene            9820..11088
                     /gene="secY"
                     /locus_tag="PC700_001481"
     CDS             9820..11088
                     /gene="secY"
                     /locus_tag="PC700_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDF2580796.1"
                     /translation="MLSKLKQAWKIKAVRKKVMYTLMMIVIFRIGTTIPVPGIDTSII
                     QKMVGGNSLLSLYNMFTGGAFSNFSLFALGISPYITASIIIQLLTVGFESLAELQKSG
                     EEGKKKINKYTKYTALALAVVQALGITLGIVRSALISNSVFFITTVVITLVSASMLVM
                     WIGDKITEKGIGNGSSVIIFAGIISRIPTDVIKISQQVKSGEVAPWVIVILAVVILLT
                     VTGVTFIQEATRKIPVQYAKRVVGRKMYGGQSSHIPMKVNQSGVMPIIFASSLLAFPQ
                     TIAMFMGPNAQAFVQKYLSMATEQGFWTYRSIEILLIIFFSYFYTTVSFNTEDISKNM
                     KNNGGFIPGIRPGEPTMDYLNRILTRLTLAGATFLAIIAMVPALTTHYMKVNMSLAGT
                     SLLIVVGVALELKRQLESNLVMRSYQGFLK"
     gene            11105..11755
                     /locus_tag="PC700_001482"
     CDS             11105..11755
                     /locus_tag="PC700_001482"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDF2580797.1"
                     /translation="MRIILLGPPGAGKGTQAAGIVEKYNIPHISTGDIFRKNIKEGTE
                     LGKKAKEYMDQGLLVPDELTVGLVTDRISQEDCKNGFMLDGFPRNVAQGEHLDIFLKN
                     AGISLDKVVNIEVDKSILVSRAVGRRICKSCGATYHVEFNPPKVEGVCDVCQGELYQR
                     ADDNEETVSKRIQVYLDETKPLVDYYSKQGIIADIKGDQAIDKVFEDIVAALGSGK"
     gene            11756..12502
                     /gene="map"
                     /locus_tag="PC700_001483"
     CDS             11756..12502
                     /gene="map"
                     /locus_tag="PC700_001483"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDF2580798.1"
                     /translation="MIILKSKKEIELLREAGKIVADTHEVLRKAISPGISTLELDKIA
                     EENIRKYNAEPSFKGYGGFPGSICASINREVVHGIPGETILQEGDIVSLDIGAYYKGY
                     HGDSAKTHGVGMISEEDRKLIEVTRESFYEGIKFAKLGYRLSDISHAVQTHVEKHGFS
                     VVRDLVGHGVGANLHEDPQVPNYGLPGKGPRLREGMVIAIEPMVNAGRYHVKTLSDGW
                     TTVTIDGKKSAHYEHTIAITEHEPLILTKL"
     gene            12524..12793
                     /locus_tag="PC700_001484"
     CDS             12524..12793
                     /locus_tag="PC700_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing protein"
                     /protein_id="HDF2580799.1"
                     /translation="MLSDNLSIGQVVKVSLGRDKGNLFFVVKIINNEYVLIADGKKRK
                     LDKPKLKKVKHLKKYDFINDEVRKRVVSGQEITDSFLRAELTKLN"
     gene            12831..13049
                     /gene="infA"
                     /locus_tag="PC700_001485"
     CDS             12831..13049
                     /gene="infA"
                     /locus_tag="PC700_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDF2580800.1"
                     /translation="MAKKDVIELEGTVSEALPNAMFKVKLENGHEILCHISGKLRMNF
                     IRILEGDKVNVELSPYDLTRGRITWRKK"
     gene            13085..13198
                     /gene="rpmJ"
                     /locus_tag="PC700_001486"
     CDS             13085..13198
                     /gene="rpmJ"
                     /locus_tag="PC700_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDF2580801.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQKQG"
     gene            13351..13722
                     /gene="rpsM"
                     /locus_tag="PC700_001487"
     CDS             13351..13722
                     /gene="rpsM"
                     /locus_tag="PC700_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDF2580802.1"
                     /translation="MARIAGVDLPREKRAEIGLTYIYGIGKATANEILAKAEINPDTR
                     IKDLSEDQVNELRKVIDDDFLVEGDLRREIALNIKRLRDIKCYRGIRHAKGLPLRGQR
                     TKTNARTRKGPRKTVSRKKKK"
     gene            13758..14156
                     /gene="rpsK"
                     /locus_tag="PC700_001488"
     CDS             13758..14156
                     /gene="rpsK"
                     /locus_tag="PC700_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDF2580803.1"
                     /translation="MAKPKKKVTRIRRRERKNIERGHAHIQSTFNNTIITLTDVHGNA
                     ISWASSGQLGFKGSRKSTPFASQMAAETAAKAAMEHGLKSVEVFVKGPGSGREAAIRA
                     LQATGLEVTMIKDVTPIPHNGCRPPKRRRV"
     gene            14188..14811
                     /gene="rpsD"
                     /locus_tag="PC700_001489"
     CDS             14188..14811
                     /gene="rpsD"
                     /locus_tag="PC700_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDF2580804.1"
                     /translation="MARYTGASCRQCRREGMKLFLKGDRCYTDKCAIVKRNYAPGQHG
                     QGRKKVSNYGLQLREKQKVKRIYGVLETQFRNLYERAENMPGKAGENLLSLLERRLDN
                     VVYRMGLASSRKEARQLVTHGHFTLNGNKVDIPSLIVKVGDVIEVKEKSRSSAKFKNL
                     VEVNSRIAPKWLEANVEGMTAKVVGVPTREDIDLEIAEHLIIELYSK"
     gene            14889..15836
                     /locus_tag="PC700_001490"
     CDS             14889..15836
                     /locus_tag="PC700_001490"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDF2580805.1"
                     /translation="MIEIEKPKVDIVELSEDYRYGKFVIEPLERGYGITIGNALRRIL
                     LSSLPGVAVNAIKIDGVLHEFSTIPGVKEDVTEIILTLKELSATIDGEGSRTLKIEAQ
                     GPCSITGADIICPPDVEILSKDLAIATLDDNAKLNMEIFVDKGRGYVSAEENKTENVP
                     IGVLPVDSIYTPVEKVSYHVENTRVGQKTDYDKLVLEVWTNGSINPQEGISLAAKVLV
                     EHLNLFIDLTEHVSSVEIMVEKEEDQKEKVLEMTIEELDLSVRSYNCLKRAGINTVEE
                     LANKSEDDMMKVRNLGKKSLEEVIQKLEELGLGLKPSEE"
     gene            15857..16198
                     /gene="rplQ"
                     /locus_tag="PC700_001491"
     CDS             15857..16198
                     /gene="rplQ"
                     /locus_tag="PC700_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDF2580806.1"
                     /translation="MAKYRKLGRETAHRNLMLRNLVTCLLRSGRIETTVTRAKETRRM
                     AEKMITLAKRGDLHARRQVLAYVMDETVVNNLFTDLAPKYAERNGGYTRIIKIGPRKG
                     DAAEMAFIELV"
     gene            16318..17151
                     /locus_tag="PC700_001492"
     CDS             16318..17151
                     /locus_tag="PC700_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HDF2580807.1"
                     /translation="MDNIVKVNNISFEYITDEAKLKAIDNLSLDVKKGEFVAIIGHNG
                     SGKSTLSKNLNAILMPTEGNILIDDMDTKEEERLWDIRQTAGMVFQNPDNQIVATIVE
                     EDVAFGPENLGIEPKEIRRIVEESLKSVGMYDLRDRQPHLLSGGQKQRVAIAGIIAMR
                     PKCIIFDEATAMLDPSGRKEVMKTIKRLNKEENITVIHITHFMEEAVEADRVVVMEKG
                     KKILEGTPREVFSKIKMLKEIGLDVPCMTELSSLLIEEGINISSDILTVDEMVMELCQ
                     L"
     gene            17139..18005
                     /locus_tag="PC700_001493"
     CDS             17139..18005
                     /locus_tag="PC700_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HDF2580808.1"
                     /translation="MSIIVKNLTHIYNEGMPFASKALDDISFEIKDRDFVGLIGHTGS
                     GKSTLIQHLNGLLKPSSGEIFINDFNITDKNLNLTEIRKRVGVVFQYPEYQLFEETID
                     KDIAFGPSNLGLEESEIHNRVKASMEAVGLDYEGFKDKSPFELSGGQKRRVAIAGVIA
                     MNPEVLILDEPTAGLDPGGRDEIFNLIKDLHEKKNMTIILSSHSMDDMAKLAKTLIVM
                     NHGSVEFMGTPREVFKSNASKLKDIGLDIPQVLELALKLREKGFDISEDILTLEEAKQ
                     EILKVVRGRGLC"
     gene            17999..18802
                     /locus_tag="PC700_001494"
     CDS             17999..18802
                     /locus_tag="PC700_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDF2580809.1"
                     /translation="MLKDITIGQYYPTSSAIHKLDPRIKLVATIVFMVSIFVVNKFWP
                     YIVVLLCLLAMIKLANIPVKYIVKGVKPLKWIILFTFLINIFFLPGDEIWSFGFLAIT
                     KQGLRQAIFMAIRLIFLVVGTSLLTLTTSPIELTDGIERLLNPFKKIGLPVHELAMMM
                     TIALRFIPTLLDETDKIMKAQMSRGADFESKNLINRAKNLVPLLVPLFVSAFRRADEL
                     AMAMEARCYRGGHNRTKMRESVISRRDYMACVFQVVYLGAIIATRFIAI"
     gene            18834..19565
                     /gene="truA"
                     /locus_tag="PC700_001495"
     CDS             18834..19565
                     /gene="truA"
                     /locus_tag="PC700_001495"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDF2580810.1"
                     /translation="MRNIKIKIQYNGKNYCGWQKQPDSLGIQGTIERAIYDITKEETS
                     LIGSGRTDSGVHAIGQIANFKINSGISIESIPMALNAKLPKDISVIEACEVNDDFHSR
                     YSAKGKTYKYLVYNSKFRNPILSEISYQVKYELDFDKMCSEAKSLLGTHDFKGFMSSG
                     SSVKDTVRTIYDIDISKKDDLITFEISGNGFLYNMVRIIVGTLVDMGRGRINEPFLDI
                     IQSKTRSRCGHTAPAQGLFLKKVHY"
     gene            19685..20116
                     /gene="rplM"
                     /locus_tag="PC700_001496"
     CDS             19685..20116
                     /gene="rplM"
                     /locus_tag="PC700_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDF2580811.1"
                     /translation="MKSYIAKPADVQRKWYLVDAEGKTLGRLATEIATVLRGKHKPTF
                     TPHVDGGDFVVVVNAEKIVLSGKKLDQKYYRYHTGYVGGLKEISYRDMMDKKPEEVIS
                     HAVSGMLPKNKLRSRMMTRLRVFAGAEHTHAAQNPEVLNFK"
     gene            20145..20537
                     /gene="rpsI"
                     /locus_tag="PC700_001497"
     CDS             20145..20537
                     /gene="rpsI"
                     /locus_tag="PC700_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDF2580812.1"
                     /translation="MANVQYYGTGRRKSSVARVRLVAGEGNILVNGRALENYFNYETL
                     IRDVKQPLVLTGNENKYDVIVKVEGGGFTGQAGAIRHGISRALLKADLDLRPALKKEG
                     FLTRDARMKERKKYGLKAARRAPQFSKR"
BASE COUNT         8265 a         2442 c         4325 g         5675 t
ORIGIN      
        1 atggatttaa tataaaaaaa agaggggggt aactcctctc ttttttgttt tttgttgaca
       61 agtagcgcta aagcatatat actaaactag tgccttgaga cagattaaca atcaagttta
      121 aggttatgat gttgtaatag aaaataaaat tgaaaaaaac agaaaaaaaa cttgaaaaaa
      181 tgtaacagat gatataaaat ataatagtgt gcttgagaga ttaagaaaat aataattaaa
      241 tcctagtgaa atacgaactg atatcgattt aaaagttaca acacacccgg agcagtgtat
      301 cggtattggt tgtacgcaac ttgaaaatta aacacgtgat aaaggaggga aaacaaaatg
      361 gctaagaatg aaaaaataag aataagatta aaatcatatg atcacaaatt attagatttt
      421 tcagcaggta aaatagttga aactgctaaa aaggctggat cacaagtttc aggacctgtg
      481 cctctaccaa cagaaaaaca agttgtaact atattaagag ctgtacataa gtacaaagac
      541 tctagagaac aatttgaaat aagaactcat aaaagactaa tcgacatagc taatccaaca
      601 cctaagacag ttgactcatt aatgagatta gacttaccag caggtgtaga tatagaaata
      661 aaattataag aacccctaag atattatcct atagatgatt atcttagaat gattactcta
      721 aaagatgtaa tccgctgtaa gaattatagg aggtgctaat atgaaaggaa tattaggcaa
      781 aaaagttggt atgactcaaa tattcactga taaaggtgta gttatacctg taacagctgt
      841 tgaagcaggg cctatggtcg ttactcaaat aaaaacagtt gataaagatg gatacaatgc
      901 tatacaaata ggttttgaag atgctaaaga aaaagcttta aacaaaccta aaaaaggaca
      961 tttagctgct gctaatgttt taaagaaaca tttaaaagaa tttagagtag attctgtaga
     1021 aggatacaca gtaggacaag aaataaaagc tgatgtattt gaagcaggtg caaaaataga
     1081 tgttactgga ataagtaaag gtaagggatt ccaaggtcca ataaaaagac atggacaaag
     1141 tagaggtcct gaaactcatg gttctagata ccacagaaga ccaggttcaa tgggagcttg
     1201 ttcttaccca ggtagagtat ttaaaaacaa aaaattagct ggacatatgg gaagcgtaaa
     1261 ggtaacagtt caaaacttag aggttgttaa agtagatgct gacaagaacc ttatattagt
     1321 taaaggagct ataccaggag ctaaaggttc agtagtaact ataaaagaag ctataaaggt
     1381 ttctaaataa tgactaacct agagaaagga ggaataacaa tgccaaaatt aaatgtacta
     1441 aatgttagtg gacaaaacgt tggagaaata gaattatcag attctatatt tggcgtagaa
     1501 gttaatggac atgttttata cgaagttgta aaaaatcaat tagcaaataa gagacaagga
     1561 actcaatctg ctaaaactag agcagaagtt agaggtggcg gaagaaaacc ttggaaacaa
     1621 aaaggaacag gtagagcaag acaaggttct acaagatctg tacaatgggt aggtggagga
     1681 gttgcttttg cacctaaacc aagaagctac aaatatacat tacctaagaa ggtaagaaga
     1741 ttagctatga agagtgcttt atcttcaaaa gttcaaaata gtgaagttat agtattagat
     1801 gcattaaaca tggatgctcc taagactaaa gaatttgctc aaatattaaa caatataaat
     1861 gctgctaaaa aagctttagt agttatagct gacaaaaacg ataacgtaat aaaatcagct
     1921 agaaatatag aaggtgttca aactgcatta gttaatacta tgaatgtata tgatatatta
     1981 aaatacgatt catttataat aactacagat gctgttaaaa aagtggagga ggtgtacgca
     2041 taatgactaa tccacatgat gtaataataa gaccagttgt aactgagcac agtatggctg
     2101 aaatgggtga aaaaaaatac acttttgttg tggctaaaga tgcgaacaaa actgaaataa
     2161 aaaaagcagt agaaaaagta ttcggagtta gtgttgataa agtaaatact ttaaactacg
     2221 atggaaaagt taaaagaatg ggtagaactc aaggaagaac agcaagcttt aagaaagcag
     2281 tagttaaatt aactgctgat agtaaagaaa tagaattctt ccaaggaatg tagtattata
     2341 acaaacgaag gagggaaaac agatggctat aaaaaagttt agaccaactt ctcctgcctt
     2401 aagacaaatg acagttttag tttctgatga aataacatgt aatcaaccag aaaaatctct
     2461 tttagttaac ttaaagaaaa atgctggtag aaacgtacat ggtagaataa ctgttcgtca
     2521 tagaggtgga ggacaaaaaa gaaaatatag aataatagat tttaaaagag ataaagatgg
     2581 tatacctgct aaggttgcta ctatagaata tgatccaaac agaactgcaa atatcgcatt
     2641 attaaactat gctgatggag aaaaaagata tatattagct ccagttggaa ttaacgttgg
     2701 agatactata ttatcaggat taggtgctga tataaaacca ggaaactgtt tagcattaaa
     2761 agatatgcca gttggtacta tcattcataa tatagaatta aagccaggaa aaggagctca
     2821 attagttaga tctgctggag tttctgcaca attaatggct aaagaaggaa aaaatgcttt
     2881 attaagatta ccatcaggtg agatgagatt agtaagcata aactgtaaag ctactatagg
     2941 tcaagttgga aatatagagc atggtaacgt agttatcggt aaagcaggta gaaaaagaca
     3001 tatgggaata agacctactg taagaggttc tgtaatgaac cctaatgacc atccacacgg
     3061 tggaggggaa ggtagatctc caataggtag accatctcct gttactccat ggggtaaacc
     3121 agctcttgga tacaaaacta gaaagaaaaa taaagcttca aataaactaa tagtatcaag
     3181 aagaactaag tagtaaattt atttttgctc ggaaaggagg aatataaatg tcaagatcaa
     3241 ctaaaaaagg gccttttgtc catgcaagac ttttaaagaa gatagaagct atgaatgcta
     3301 gtggaaataa agaagttata aaaacttggt caagatcttc aactgtattt ccacaaatgg
     3361 tagaaaacac tatagctgtt catgatggaa gaaaacatgt tccagtatat ataacagaag
     3421 atatggttgg acataaatta ggtgagttcg ttcctactag aactttcaaa ggacataagg
     3481 atgacgaaaa atctaataag agaaaataat taaattatga ctaagaaagg aggaataaca
     3541 atggaagcaa aagctactgc taaatacgta cgtgtatcac caagaaaagc aggccaaata
     3601 tgtgaccttg ttagaggaaa gaatgttgat gaagcattag caatattaaa gtttactcca
     3661 agaggagcag cttcaataat agctaaggtt gtaaaatcag ctaaagctaa cgcagaaaac
     3721 aatcacgaaa tggatactga aaaattatat atagcatcaa tagttgctaa tcaaggacca
     3781 acaatgaaga gattcatgcc tagagctatg ggtcgtgcaa ctacaataag aaaaagaact
     3841 tctcatatag aggttgttgt taaagaaaaa aaataattaa tagataggag ggaaagtaat
     3901 gggtcaaaag gttaatccac acggcttaag ggtcggtgtt ataaaagatt gggactcaag
     3961 atggttcgct actgataaaa aagagttcgg aaacttatta ttagaagacc ataatatacg
     4021 taaattctta aagaaaagat tatactcagc tggagttgct aagatagaaa tagaaagatc
     4081 agctaacaaa ataaaaatgg acttacacgt tgctaagcct ggtgtagtta taggaagagc
     4141 tggtgcagga atcgaagcat taaaagctga attagaaaaa atgacaaaga aaactataat
     4201 agttaatata gtagaagtaa gaagtacaga taaaaatgct caattagtag ctgaaaatat
     4261 agctttagct atagagagaa gggttgcttt cagaagagca atgaaacaag ctatacaaag
     4321 agcaatgaaa tctggtgcta aagggataaa agtttctgct tcaggaagat taggtggagc
     4381 tgaaatggct agaactgaag gttatagtga aggaaatgtg ccactacaaa ctttaagagc
     4441 agatatagat tatggtttcg ctgaagctga tacaacttat ggaaaaatcg gtataaaagt
     4501 ttggatatgt aatggtgaag ttttaccaac tagagacggt gtaaatccaa gagaagaaag
     4561 cagaaagagc gatagaagag acaacaagag agataataga agaaacgata gaagaggaaa
     4621 tgacagaaga ggaaatgata acagaggaaa ctacagagga caaagacctc aaggtggatc
     4681 aagacctcaa agaactgaaa acaaaggaaa ttaataatag ttaggttcaa ggaaggagga
     4741 aaaatactca tgttaatgcc aaaaagagta aagcgtcgta gagttcatag aggaagcatg
     4801 gctgggcaag ctcataaggg taataaagtt acttatggtg agtttggatt agtagcatta
     4861 gaagcttctt ggataacttc taatcaaata gaagctgcca gaatcgcgat gactagatat
     4921 ataaaaagag ggggaaaggt ttggataaaa atattccctc ataaaccagt aacaagaaaa
     4981 ccagcagaga ctcgtatggg tgcagggaaa ggttctccag aatattgggt agctgtagtt
     5041 aagccaggaa gagttatgtt tgaattagca ggtgtttctg aagataaagc aagagaagct
     5101 atgagacttg ctgcacataa attaccaatc aaatgtaaat ttgttaaaaa agaagattta
     5161 gaagtaaagg gtggtgaata ggatgaaagc taaagaatta agagatttaa caagcgaaga
     5221 gctaatgaac aaattaaacg acttcaaaag tgaattattt agcttaagat tccaattagc
     5281 tactggtcaa ttagaaaata cagctagaat aaagtttgtt aagaaggata tagcaaaagt
     5341 taaaactgtt cttgctgaaa gaaagttata cgaaactaga gcttaatttg gaaaggaggc
     5401 tttttaaaca tggaaagagg aagaagaaaa gttagaatag gccgtgttgt tagtgataag
     5461 atggataaaa caatagtagt agctgttgaa gaattcgtac gtcatccact atataataaa
     5521 cgtgttaaga gaactaagaa attcaaggct catgatgaaa agaatatatg taacatcgga
     5581 gatagagtta aaataatgga aactagacca ttatctaaag ataagagatt cagactagtt
     5641 gaagttgttg agaaagttaa gtagtttttg aagaaggagg gattacgata tgatacaaca
     5701 agaatcacgt ctaagagttg ctgataactc aggagctaaa gaacttttat gtatacgtgt
     5761 tctaggcgga agtaaaagaa gatatggtaa cataggcgac gttatagttg ctactgttaa
     5821 aagtgcaaca cctggtggag ttgtaaaaaa aggtaaggtt gttaaagctg ttatagtaag
     5881 aagcaaacaa ggcgtaagac gtaatgacgg aagctatata tcttttgatg aaaatgctgc
     5941 tgttataata aaggacgata aaactccagt aggaactcgt atattcgggc ctgttgctag
     6001 agagttaaga gacaatgaat tcatgaaaat agtttctctt gctccagaag tactataatt
     6061 aaggaggtgc aataggacat gatgcgtgtt aaaaaaggtg acactgttgt agttatagca
     6121 ggtaaagata aaggtaaaaa aggttcagtt ttaaaggtat atcctaaaac tagcaaagtg
     6181 ttagttgaag gtgtaaatgt aataactaaa catcaaaaac caagtgctat gaaccaacaa
     6241 ggtggaataa taaataaaga agccccaata cacatatcta atgtaatgcc atttgatcct
     6301 gaaacaggaa aaggcgttag agtaagatat gaagtaaaag atgggaacaa agtaagagta
     6361 tcagcaaaga gcggaaaaga attataataa atattgcttg aaaggaggga ccttaaatgg
     6421 cttctagatt acaagaaaaa tacatgaaag aagttgctcc tgctttaatg gagaaatttg
     6481 gatacaaaaa cgtaatggag atacctaagt taaataaaat agttattaac atgggtatag
     6541 gtgacgctag agaaaatcca aaaggattag aaaaaggcgt tgaagaatta gaaatgatat
     6601 caggacaaaa gcctgttata actaaagcta gaaaatctgt tgctaacttc aaattaagag
     6661 aaggaatgcc aataggaaca aaagttacat taagagctga caaaatgttc tactttatgg
     6721 acaaattagt ttcagtttct ttaccaagag ttagagattt tagaggagtt aaccctaatg
     6781 cttttgatgg tagaggaaat tatgctttag gagttaaaga acaattaata ttccctgaaa
     6841 tagaatatga taaaatagat aaagtaagag gaatggatat aatatttgtt actacagcaa
     6901 aaactgacga agaagctcgt gaattattaa aattattagg aatgccattt tctaagtaag
     6961 caaaggaggg attcccgtgg ctagaaaagc gatggttgtt aagcaacaaa gaaagcaaaa
     7021 gtacgctact agagaataca ctagatgtac tatatgcgga agaccgcatt cagttttgaa
     7081 aaaatttggt atatgccgta tatgctttag agaattagct tataaaggtc aaatacctgg
     7141 tgttagaaaa gcaagctggt agaagcttga ttagaatgga aggagggcaa caaaatgaca
     7201 atgacagatc caattgcaga tatgcttact cgtataagaa atgctaatgt tgttaagcat
     7261 gaaactgtag atgttccagc ttctaatatg aagaaagaat tagctagaat cttattagaa
     7321 gaaggtttca taagaggtta tgatgttata gaagatggaa aacaaggtat cataagaata
     7381 caattaaagt acggacaaga aggcgaaaga gttataacag gtcttaagaa aatatctaag
     7441 cctggaatga gagtttacgc tgctaaccat gagataccaa aagtattaaa cggattagga
     7501 atatcagtta tatctacttc aaaaggaata ttaactgaca agcaagctag aaaagaaaat
     7561 gttggtggag aagtaatctg ctacgtttgg taattaatat gaacaatgta aggaggtgcg
     7621 accatgtcaa gaataggtgt taagccaata ataataccag caggtgttga agttactata
     7681 gctgaaggaa atttagttac agtaaaaggt ccaaaaggaa ctttaactaa acaattaagt
     7741 gctgagttaa acataaaaaa agaagaaaat acaataatgg ttgaaagacc aactgataat
     7801 aaaaaacata gatctttaca cggactaact agaactttat tagataatat ggttgtaggt
     7861 gtaaatactg gtttcgaaaa gaaattagaa ttaaaaggtg ttggatatag agctcaaaaa
     7921 caaggaaaga aattagttat gaacttaggt ttctctcatc cagttgagat ggaagatcca
     7981 gaaggaataa ctgttgaagc tccaaaccaa acagagttaa tagtaaaagg tatagacaag
     8041 caattagtag ggaattatgc tgctaagata agagcttgga gaaagccaga gccatacaaa
     8101 ggtaaaggta taaaatacgt tgatgaagta atcagacgta aagaaggaaa aactggtaaa
     8161 aaataatacc aaaacttaaa cgctagaaag gagtgagcta ggtgttaaaa aaggctgata
     8221 aaaatgctaa tagacttcaa agacataaga gagttcgtag aaaaataagc ggtacttctc
     8281 aaagaccaag attatgtgta tttagaagtg ctaacaatat atatgctcaa ataatagatg
     8341 acacaaaaag agtaactcta gttgctgcat cttctttaga agcagaagtt aagagtgctg
     8401 tcaatcatac aggaaacaag gaagcagcta agaaagttgg agaattagtt gctaaaaaag
     8461 ctgttgaaaa aggaatcact gaagttgtat ttgacagagg tggatactta tatcatggaa
     8521 gaattcaaga attagctgaa ggtgctagag aagctggtct taagttctaa tacgaaggag
     8581 ggaaaaatac atgctacgtc gtaagccgat agatgcagga caacttgact tacaagaaaa
     8641 agttgttgaa gtaagacgtg ttactaaggt tgtaaaaggt ggtagaaatt tcagatttgc
     8701 agcattagta gttgttggag atgaaaacgg acacgttggt ataggtgctg ggaaagcaat
     8761 ggaagtacca gacgctataa aaaaagcagt tgaagatgct aagaaaaatc taatagtagt
     8821 gccaatagtt gggactacaa ttcctcacga agtacgcgga cactttggtg ctggaaatat
     8881 attaataatg cctgctgtag aaggtacagg agtaatagct gggggacctg ctagagccgt
     8941 acttgagcta gctggactaa aagacgttag agctaaatct ttagggtcta acaatccaag
     9001 aaatatggta aatgctacaa tagaaggact taattctcta aaaacagttg aagatatagc
     9061 taaacttaga ggtaaaaaag ttgaagaact tctagggtaa ggaggtagtg taaaatggct
     9121 aaattacaaa taaagttagt tagaagtgtt ataggaacta ctcctaacca aaaaaagaat
     9181 gttgaagcgt taggattaag aaaaagagaa caagttgttg taaaagaaga caatgctcaa
     9241 acaagaggta tgataaataa agtaagtcac ttattagaag taactgaaat agctgaataa
     9301 tttttataaa gtagtgcaag gaggtgcaat ccatgaagtt acatgagtta aaacctgctg
     9361 aaggtgcagt aagagctaag agaagattag gtagaggtac tgcaactgga caaggtaaaa
     9421 ctgcaggacg tggacaaaaa ggtcaatggt ctcgttctgg tggtggagta agagtaggat
     9481 ttgaaggtgg acaaatgcca ctagctagaa gacttcctaa gagaggtttc aataacatat
     9541 ttaagaaagt ttacacagaa gttaatgttg aagttttaaa cagatttgaa aatggaacag
     9601 aaataactgc agaattatta aaatctacta aaactataag caaaataggt aaagacggaa
     9661 ttaaaatctt aggtgaaggt aatttagaga aagctttaac tgttaaagct gctaaattta
     9721 cagcttcagc tcaagaaaaa atagaaaaag ctggtggaaa agcagagtta gtataataac
     9781 ctgctaatct cctagccagt acaggcgggg tgaattagcg tgctgtcaaa attaaaacaa
     9841 gcttggaaga ttaaagcagt aagaaaaaaa gtaatgtata cacttatgat gatcgtgata
     9901 ttcagaatag gtactactat accagttcca ggcatagaca ctagtattat acaaaagatg
     9961 gtaggcggca atagtctact ttctctgtat aatatgttta ctggtggagc atttagtaac
    10021 ttttcactat ttgctttagg tataagtcca tatatcactg catcaatcat aatacaactt
    10081 ctaacagtag gttttgaaag tttagcagaa cttcaaaaat ctggtgaaga aggaaagaaa
    10141 aaaatcaata agtacactaa atatacagca ctagctctag cagttgtaca agctttggga
    10201 attacattag gaattgtaag aagtgcttta atatcaaata gtgtgttttt tatcacaact
    10261 gttgtaataa cattagtttc agcaagtatg ttagtaatgt ggattgggga caaaattact
    10321 gaaaaaggaa tcggtaatgg tagttcagta ataatatttg caggtataat atcaagaata
    10381 cctacagatg ttattaaaat atcgcaacaa gttaaatctg gcgaagttgc accttgggtt
    10441 atagtgattc tagctgtagt tatattactt acagtaacag gagttacatt tatacaagaa
    10501 gcaactagaa agatacctgt acaatatgct aaaagagttg ttggaagaaa aatgtatgga
    10561 ggacaaagtt ctcatatacc aatgaaagtt aaccagtcag gagttatgcc tataattttt
    10621 gctagttcac ttttagcttt ccctcaaaca atagctatgt ttatgggtcc aaatgctcag
    10681 gcttttgttc aaaaatactt aagtatggca acagaacaag gattttggac atatagatca
    10741 attgagattc tacttataat tttcttctca tatttttata caactgtatc atttaataca
    10801 gaagacatat ctaaaaatat gaaaaataat ggtggattta taccaggtat aagaccaggt
    10861 gagcctacta tggattattt aaatagaatt ttaactagat taactttagc tggagctact
    10921 ttcttagcaa taattgctat ggtgccagct cttacaacac attatatgaa agtaaatatg
    10981 agtctagctg ggacttcatt gctaatcgtt gttggagttg ctttggagct taagagacag
    11041 ttagagtcaa acttagttat gagaagctat caaggtttct taaaataaaa tggagatgga
    11101 taatatgaga ataatattac ttggacctcc aggtgctggt aaaggtactc aggcggcagg
    11161 aatagtagaa aaatacaata tacctcatat atcaacagga gatatattca gaaagaatat
    11221 aaaagaggga acagaacttg gaaaaaaagc taaagaatac atggaccaag gtttattagt
    11281 accagatgag ttaactgtag gtttagttac tgatagaata tctcaagaag attgtaaaaa
    11341 tggatttatg ttagatggat ttccaagaaa tgtagcacaa ggagaacatt tagatatctt
    11401 cttaaaaaat gctggtatat cactagataa agttgtcaat attgaagttg ataagagtat
    11461 attagtgtct agagcagttg gtagaagaat atgtaagtct tgtggagcta cttaccatgt
    11521 tgagtttaat cctcctaaag tagaaggtgt atgtgatgta tgccaaggag aattatatca
    11581 aagagctgat gataatgaag aaactgtatc taagagaata caagtttatc tagatgaaac
    11641 taagccttta gtagattatt atagcaaaca aggtataata gcagatataa aaggtgatca
    11701 agcaatagat aaagtatttg aagatattgt cgcagcctta ggaagtggaa aataaatgat
    11761 tatcttgaaa tcaaaaaaag aaatagaact tttaagagaa gcaggtaaaa tagttgctga
    11821 cactcatgaa gttttaagaa aagctatttc tccgggaata tctactttag aattagataa
    11881 aatagctgaa gagaatataa gaaaatacaa tgcagagcct tcttttaaag gatatggagg
    11941 ttttccagga agtatatgtg cttctatcaa tagagaggta gtacatggta ttccaggaga
    12001 aactatatta caagaaggtg acattgtaag tttggatata ggagcctatt ataaaggata
    12061 tcatggtgat tctgcgaaaa ctcacggagt aggcatgata tctgaagaag ataggaaatt
    12121 gatagaagta actagagaaa gcttctatga aggaataaaa tttgctaaat taggatatag
    12181 actttcagat atctcacacg cagtacaaac acacgttgag aaacatggtt tctcggtagt
    12241 tagagattta gtgggtcatg gagttggagc aaatcttcat gaagacccac aagtacctaa
    12301 ttatggactc ccaggaaaag gaccaagact tagagaagga atggttattg ctatagagcc
    12361 aatggttaat gcaggtcgtt atcatgtaaa gactctatca gatgggtgga cgactgttac
    12421 aatagatgga aaaaaatcag ctcattatga gcatacgata gcaattactg aacatgaacc
    12481 tttgatattg acaaagttgt aatttaaaaa gtaggtgaaa aaagtgctat cagataattt
    12541 aagtataggt caagttgtta aagtttcatt aggacgagat aaaggaaatt tattctttgt
    12601 agtcaaaata attaataatg aatatgtatt aatagctgat ggaaaaaaga gaaaacttga
    12661 caaacctaag ttaaagaaag tgaagcactt aaaaaaatat gattttatta atgatgaagt
    12721 tagaaaaaga gtagtatctg gacaagagat tacagattct tttttaagag ctgaacttac
    12781 taagttaaat tagataattt aacttattga aatggagggc tggttagtta atggccaaaa
    12841 aagatgttat agaattagaa ggtacagttt cagaagcttt acctaatgct atgttcaaag
    12901 ttaaactaga aaatggacat gagatattat gccacatttc tggaaagcta agaatgaact
    12961 tcataagaat tcttgaaggt gacaaggtga atgttgaact ttctccatat gaccttacaa
    13021 gaggaagaat tacttggcgt aagaagtaga ctatattaat atttagtcta aggagggatt
    13081 aataatgaag gttagaccat cagtaaaacc aatatgtgaa aaatgtaaag taataaaaag
    13141 aaaaggaaaa gtaatggtta tctgtgaaaa tccaaagcat aagcaaaagc aaggataaga
    13201 aattattact agcattttgt taaattttat agtataatac tatattgtga tgttaacaaa
    13261 agaacgttaa aatcgttgaa caatctggcg taggattggc atatgatgtg aagttttaaa
    13321 cactaaaaac tatgtgtagg aggtgcgatc atggcaagaa tagctggggt agatttacct
    13381 agagaaaaaa gagcggagat aggcttaact tatatatatg gtataggtaa agcaactgct
    13441 aacgaaatat tagctaaggc tgagataaat ccagatacta gaataaaaga tttatcagaa
    13501 gatcaagtta atgagttaag aaaagttata gatgacgatt tcttagttga aggtgactta
    13561 agaagagaaa ttgctttaaa cataaagaga ttaagagata taaaatgtta tagaggaata
    13621 agacacgcta aaggtcttcc attaagagga caaagaacta agactaatgc tagaactaga
    13681 aaaggtccta gaaaaacagt atctcgtaaa aagaaaaaat aaataaataa taactagatg
    13741 aaggagggaa aaggaaaatg gctaaaccaa aaaagaaagt tacacgtatt agaagaagag
    13801 aacgtaaaaa tatagaacgt ggtcatgctc atatacaatc aactttcaat aatacaataa
    13861 taactttaac tgacgttcac ggaaatgcta tatcttgggc aagttctgga caattaggat
    13921 tcaaaggatc aagaaaatca actccatttg catctcaaat ggctgctgaa acagctgcaa
    13981 aagctgcgat ggaacacgga ctaaaaagtg ttgaggtatt cgtaaagggg ccaggttcag
    14041 gaagagaagc tgcaataaga gctttacaag caactggact agaagtaact atgataaaag
    14101 acgttactcc aatcccacat aacggatgca gaccaccaaa gagaagaaga gtgtaattaa
    14161 taagagtaca ataggaggtg tagataaatg gcaagatata caggtgcatc atgtagacaa
    14221 tgccgtagag agggaatgaa attattcctt aaaggtgata gatgttatac agacaaatgt
    14281 gctatagtaa aaagaaacta tgctccaggt caacatggcc aaggaagaaa gaaagtttct
    14341 aactatggat tacaattaag agaaaaacaa aaagttaaaa gaatatatgg agttttagag
    14401 actcaattta gaaatttata tgaacgtgct gaaaatatgc ctggtaaagc aggtgagaac
    14461 ttattaagtt tattagagag aagattagat aatgtagttt acagaatggg actagcatct
    14521 tctagaaaag aagctagaca attagtgact catggtcatt tcactttaaa tggaaataaa
    14581 gtagacatac cttcattaat agttaaggtt ggagatgtta tagaagttaa agagaaatca
    14641 agatcttctg caaaattcaa aaatttagta gaagttaact caagaatagc tcctaagtgg
    14701 ttagaagcta atgtagaagg aatgacagca aaagtagttg gtgttccaac aagagaagat
    14761 atagatcttg agatagcaga gcacttaatc atagaacttt actctaaata ataagcacac
    14821 ttttaaaaat tattttaaaa ttttgtttac cctcagtgga ttactgaatt ttaaggaggg
    14881 ttttgtccat gatagaaata gaaaagccaa aagtagatat agttgagctt agcgaagact
    14941 atagatatgg taagtttgtt atagagcctc tagaaagagg atatggaata actataggta
    15001 acgcattaag aagaatatta ttatcatcat taccaggtgt agcagtgaat gctataaaaa
    15061 tagatggagt tcttcatgaa ttttcaacaa tacctggtgt taaagaagat gttactgaga
    15121 taatattaac tttaaaagag ctttcagcaa ctatagatgg tgaaggaagt agaacactta
    15181 aaatagaagc tcaaggacca tgctctatca caggtgcaga tataatctgt cctccagatg
    15241 ttgaaatatt aagtaaagat ttagcaatag ctacattaga tgataatgct aaacttaata
    15301 tggagatatt tgtagataaa ggtagaggtt atgtttctgc tgaagaaaat aaaacagaga
    15361 atgttccaat aggtgtttta cctgtggatt caatatatac tcctgttgag aaggttagtt
    15421 atcatgtgga aaacacaaga gttggtcaaa aaacagatta tgataaatta gtactagaag
    15481 tttggactaa tggtagtata aatcctcaag agggaatatc attagctgca aaggtattag
    15541 ttgagcattt aaacttattt atagacttaa ctgaacatgt aagtagtgtt gaaatcatgg
    15601 tagaaaaaga agaagatcaa aaagaaaaag ttcttgaaat gactatagaa gaattagatt
    15661 tatcagttag atcatataac tgtttaaaga gagcgggaat taatacagtt gaagaattag
    15721 ctaataaatc tgaggatgat atgatgaagg ttagaaacct aggtaagaag tcattagaag
    15781 aggtaataca aaaactagaa gaacttggat tgggattaaa accaagtgaa gaatagcgag
    15841 caaaggaggg ataagaatgg ctaagtaccg taaattagga cgtgaaacag ctcacagaaa
    15901 ccttatgtta agaaacttag taacttgctt actaagaagt ggaagaatag aaactacagt
    15961 gactagagcg aaagaaactc gtagaatggc tgaaaagatg ataactcttg ctaagagagg
    16021 agatcttcat gctagaagac aagttttagc ttatgttatg gatgaaacag tagttaataa
    16081 cttattcaca gatttagctc caaaatatgc tgagagaaat ggtggataca ctagaataat
    16141 aaaaataggg ccaagaaaag gcgatgctgc tgaaatggct tttatagaat tagtatagta
    16201 aggaaaggat taggcttgat ggcttaatct tttttttatt tttttgacta aggtatgata
    16261 taataatgtg taaaatgatt tttataagtt attgcttact aaaaggagta tgagttaatg
    16321 gataatatag taaaggtaaa taatatttcg tttgagtata ttacagatga agcaaaactt
    16381 aaagcaatag ataatttaag cctagatgtt aaaaaaggag aatttgttgc gataatagga
    16441 cataatggtt ctggtaagtc tactttatct aaaaacttaa atgctattct tatgcctact
    16501 gaaggaaata ttcttattga tgatatggat actaaagaag aagaaagatt atgggatata
    16561 agacaaactg caggcatggt atttcaaaat ccagataatc aaatagttgc tactatagta
    16621 gaagaagacg ttgcatttgg acctgagaac ttaggtatag aaccaaaaga aataagacga
    16681 attgtagagg agtctttaaa aagtgtaggt atgtatgatt taagagatag acagcctcat
    16741 ttgttatcag gaggtcaaaa gcaaagagtt gcaatagcag gaattattgc tatgaggcct
    16801 aaatgtataa tatttgatga agcaacagca atgttagatc catctggaag aaaagaagtt
    16861 atgaaaacaa taaaaagact taataaggaa gaaaatataa ctgttataca tataacgcac
    16921 tttatggaag aagcagtaga agctgataga gttgtagtta tggaaaaagg aaagaaaatc
    16981 ttagagggaa ctcctagaga agtattcagt aaaataaaga tgttaaagga aatcggttta
    17041 gatgtacctt gtatgacaga gttatctagt ttattaatag aagaaggtat aaatattagt
    17101 agtgatatat taactgtgga tgagatggtg atggaattat gtcaattata gtaaagaatt
    17161 taacacatat atataatgaa gggatgcctt ttgcaagtaa ggcacttgat gatatttctt
    17221 ttgagattaa agatagagat tttgttggtc ttataggtca tacaggctca ggaaaatcta
    17281 cattaataca acatttaaat ggattactaa agccttcctc aggagaaata tttataaatg
    17341 attttaatat aacagataaa aacttgaatt taactgagat tagaaaaaga gtaggggttg
    17401 tatttcaata tccagaatac caattgtttg aagaaacgat tgataaagat atagcttttg
    17461 gaccctctaa tttaggctta gaggagtctg agatacataa cagggtaaaa gcatcaatgg
    17521 aagctgtagg acttgattat gagggattta aagacaaatc tccttttgaa ttatctgggg
    17581 gacaaaagcg tagagtagca attgctggag ttatagccat gaacccagaa gttctcatat
    17641 tggatgaacc aactgctggt cttgaccctg gtggaagaga tgaaattttt aacttaataa
    17701 aagacttaca tgaaaaaaag aatatgacta taatactatc atcacatagt atggatgata
    17761 tggcaaagtt agcaaaaact ttaattgtta tgaatcatgg tagcgtagaa tttatgggaa
    17821 ctcctagaga agtgtttaaa tcaaatgcta gtaaattaaa agatatagga ttagatattc
    17881 cacaagtttt agagcttgca ttgaaattga gagaaaaagg ctttgacatc agtgaggata
    17941 tattgacttt agaagaagca aaacaggaaa tattaaaagt tgtgagagga cgaggattat
    18001 gttaaaagat ataactatag ggcaatacta cccaacaagt tctgctattc ataaattaga
    18061 tccaaggata aagcttgtag cgacaattgt atttatggta tctatatttg tagtaaataa
    18121 attttggcct tatatagtag ttttattatg tttattagct atgataaaat tggctaatat
    18181 accagtcaaa tatattgtta agggtgttaa acctcttaaa tggattattt tgtttacatt
    18241 tcttataaat atttttttct taccaggtga tgaaatatgg tcatttggat ttttagctat
    18301 tacaaaacag ggtctaagac aagcaatttt tatggcaata agattaatat ttttagtagt
    18361 tggtacatca ttgcttactt taacaacttc accaatagaa ttaactgatg gaatagagag
    18421 attgttgaat ccatttaaga aaattggttt gccagttcat gaacttgcaa tgatgatgac
    18481 aattgcttta cgttttattc ctacattgtt agatgagaca gacaaaataa tgaaggctca
    18541 gatgtctaga ggtgcagatt ttgaaagtaa gaatcttata aatagagcaa agaatttagt
    18601 accacttttg gtacctttat ttgtaagtgc atttagaaga gctgatgaat tggctatggc
    18661 catggaggcc agatgttaca gaggtggaca taataggact aagatgagag agtctgttat
    18721 atcaagaagg gattatatgg catgtgtatt tcaggttgtt tatctgggag caattatagc
    18781 tactagattt atagcaattt aattaataag ttatagatat aatgaggtaa agtatgagaa
    18841 atataaaaat taaaatccaa tacaatggta agaattattg tggatggcaa aagcaacctg
    18901 attctttagg gatacaaggt acaattgaaa gggctatata tgatataaca aaagaagaaa
    18961 ccagtcttat agggtctggt agaactgatt caggagttca tgctattggt cagattgcaa
    19021 attttaaaat taattcaggt ataagtatag aaagtatacc aatggcacta aatgccaagt
    19081 tgccaaaaga catatcagtt atagaagcat gtgaagtaaa tgatgatttt cactccagat
    19141 acagtgcaaa aggtaaaaca tataaatatt tagtatataa tagcaagttt agaaatccaa
    19201 tacttagtga gatatcatat caagtaaaat atgaacttga ttttgataaa atgtgttcag
    19261 aagcaaaaag tttattagga acacatgatt ttaaaggttt tatgagttct ggttcatctg
    19321 taaaagatac agttagaacg atatatgata ttgatataag taaaaaagat gacttgatta
    19381 cttttgagat tagtggtaat ggttttttat ataacatggt gaggattatt gttggaacac
    19441 ttgttgatat gggtcgtggc agaattaacg aaccattttt agatataata caatcaaaaa
    19501 ctaggtcaag atgtggacat acagctccag cacaaggatt atttctcaaa aaagttcatt
    19561 attaacttga cacaccaggc tacatgtatt aaaatatatg agaatgtgtt aataagtcca
    19621 cttgccccgg acttgacata aacggtaaaa aaattttatg aaaagtagtt aaggagggac
    19681 agttatgaaa agttatatag ctaagccagc tgatgtacaa agaaaatggt acttagttga
    19741 tgctgaagga aaaacattag gtcgtttagc aacagaaatt gcgacagtat tgagaggtaa
    19801 acataaacca acatttactc ctcacgttga tggtggagat tttgttgttg tagtaaatgc
    19861 agaaaaaata gttttaagtg gtaagaaatt agatcaaaaa tattacagat accatactgg
    19921 ttatgtaggt ggattaaaag aaatatctta cagagatatg atggataaga aaccagaaga
    19981 agttatatca catgctgtaa gcggtatgtt acctaaaaat aaattaagaa gcagaatgat
    20041 gactagatta agagtatttg caggtgctga gcacactcat gcagctcaaa atccagaagt
    20101 tttaaacttt aaataagatt aataggtgaa aggaggagtt tataatggct aacgttcaat
    20161 attatggaac tggaagaaga aaaagttctg ttgctagagt aagactagtt gcaggtgaag
    20221 gaaatatatt agtaaatgga agagcattag aaaattattt taattatgag acattaataa
    20281 gagatgttaa acaaccatta gttttaactg gtaatgaaaa taaatatgat gttatagtaa
    20341 aagttgaagg tggaggattc actggacaag ctggagctat aagacatgga atatctagag
    20401 ctttattaaa agctgattta gatttaagac ctgctttaaa gaaagaaggt ttcttaacta
    20461 gagatgcaag aatgaaggaa agaaagaaat acggattaaa agcagcaaga agagctccac
    20521 aattctcaaa aagataatta cttatttttt tggaaaagca aaatccttta tttactgtta
    20581 ttcagaaagt attggtattt taaaaatgtt gggttaagtt aattattgat atataataaa
    20641 ataataagat atcttattag gatgtttttc attaagaaga acatcctttt tattttgact
    20701 atttaat
//
LOCUS       DAMJWP010000047        20674 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.048, whole genome shotgun sequence.
ACCESSION   DAMJWP010000047 DAMJWP010000000
VERSION     DAMJWP010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20674
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.048"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            78..194
                     /gene="rrf"
                     /locus_tag="PC700_001498"
     rRNA            78..194
                     /gene="rrf"
                     /locus_tag="PC700_001498"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            649..1719
                     /locus_tag="PC700_001499"
     CDS             649..1719
                     /locus_tag="PC700_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2580813.1"
                     /translation="MADIRLENVTKSYNKKNTVIENLNLTIEDGSFTVLVGPSGCGKS
                     TTLRMIAGLEDITSGKLKIEENDVTKTDASKRDIAMVFQNYALYPHMTVRENIEFGLK
                     NKKVEKEKREKLITEVIDVVGLREYLNVKPGNLSGGQRQRVALARAMVKNPKVFLMDE
                     PLSNLDAKLRNQMRTELIQLHQKLKSTFVYVTHDQVEAMSMADKIVIMNKGKIMQIGS
                     PKKIYQNPKNLFVAQFIGSPCMNILDIKNDIKFGFRPEKATLESINIDGKNDDVFSLF
                     GKVVTKEVLGNETIYCLSVLNREIRVKTENDIFDIGRTLRISVLEKDLYYFDKNGERI
                     EDVKVCKSMYSKIKGDIYEKAI"
     gene            1703..2572
                     /locus_tag="PC700_001500"
     CDS             1703..2572
                     /locus_tag="PC700_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDF2580814.1"
                     /translation="MKKQFNKAYLYITPAIIGTLLFIVYPIIKTISLSFKSGSLLSST
                     FENVGLNNYTELLKNPEFIQTITNTAIYTFFMVTISISLAIVLAVWLNKNSIIHNLTQ
                     SIIFTPHVISLVSVAVLWLWIMEPQYGFLNWILSTLHLPTSKWLSSESSALMSLILIG
                     IWKTLGYNILIVLSGLKSIPNYIYEASKLDNANKITNFFKVTLPLLSPTIFFLMITTT
                     TASFQVFDEISIMTQGGPVGSTNMLSYYIYESGFIYYDIGQASAASMFLLAIVMIATY
                     ANFRLLSKKVHYQ"
     gene            2616..3476
                     /locus_tag="PC700_001501"
     CDS             2616..3476
                     /locus_tag="PC700_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDF2580815.1"
                     /translation="MDIIQPIKKDKVDIKKIASKSMEILILLLLSAIFIFPFIWMLST
                     SFKNPQEMMQLPPTIIPNDIVLENYSTAWHSGPFFRYLLNSVFVTGSVLILQFLVMVP
                     AAYAFSKIKFKGEKMLFGLLLVGLMIPPQVTFLPVYMMMSKFGFINTYIPLIIPFMTS
                     SFGIFLLRQNFMQISDETIEAAKLDNASDLKIMFRIMVPMAKPAVITFILFNFIYHWN
                     NYFWPLVMTNTDAIRTLPIGVALLKSSEGITAWNVIMAGNIILILPIILVYIFANKKV
                     KEAFMYSGIK"
     gene            3507..4811
                     /locus_tag="PC700_001502"
     CDS             3507..4811
                     /locus_tag="PC700_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2580816.1"
                     /translation="MKLKRKLATGLAITLLLGSLVGCSNSNDANASKGEKTDSNITKI
                     DFWAPLGGTNGETAQKMVDQFNSEHKDVQVNMLKQKDYYENATKLQAALTSKDQPDVT
                     LLEITQTGTFASAGALVDMSKYFDKTYQERFFSGLLTNSYYEDKFVGMPFNRSTPILY
                     INRDMATKAGLDPSGPKSWEELKDYASKMTNKSEDTYGFETPIDIWFYEAMVMQSGGE
                     ITNGKKVAFNNEAGQAPVKFWQDMMKAGIMKMPPGEDYNAWDVAKQDFVNGKVGMIFT
                     STADLAGLMQQTEGKFEISTAFLPKNQKYATPTGGANLVMLEGGTDKEKEASAEFIEW
                     MTQTDKIVQFSSSTGYLPTTEDATNSEKLQTLYKEKPQYKVATDQLQYAVALPMLNGY
                     KEATDKLMDEIKKGLTDLNANPKDVVSKGTSAMQAVIDKTNK"
     gene            4874..6136
                     /locus_tag="PC700_001503"
     CDS             4874..6136
                     /locus_tag="PC700_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HDF2580817.1"
                     /translation="MTKVIVLSVDSLFEKDLEFVKNLPNFKSILENCSIVKNINCVYP
                     TLTYPCHTSMVTGVYPKKHGIYHNEKFDPNKENKDWYWYSKDIKVKTIIDVAKENNLT
                     TSSVLWPVMGANPNIDYNIAEIWAPSREDDPRETFERSSSKVIMDGIYNRHCHYIDWK
                     LEPNMDLFGVNCSVDIIKEYKPDLMLIHLATLDHTRHNYGLFNDEVDKALKMNDKWLG
                     DIIQATKDAGTYKDTNFIILGDHGHLRVDKVINPNVLLKSNGFIKVENGEVKDFDAYV
                     NSSGISAQIIVNNLDRLTELRELLEEFKEELFIENIFTKEEASNLGLEGDFEMVIEGL
                     EGISFGNDFEGSIIKDSDIKDYKFAVATHGHLPTKGNRPPFIAFGPNIKGGVVIEEGD
                     LRAHAATILKMFDLKLDGVEKEAFDFIM"
     gene            6323..8293
                     /gene="uvrB"
                     /locus_tag="PC700_001504"
     CDS             6323..8293
                     /gene="uvrB"
                     /locus_tag="PC700_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HDF2580818.1"
                     /translation="MDFKIKSDFKPTGDQPEAIKSIVDSINRNEKFSTLLGVTGSGKT
                     FTMANIIQQVKKPTLIMAHNKTLAAQLYSEFKEFFPDNAVEYFVSYYDYYQPEAYVAH
                     SDTYIEKDASINDEIDKLRHSATASILERRDTIIISSVSCIYGLGDPKDYKELMLSIR
                     PGMQRDRDDVIKRLIEIQYERNDINFTRGTFRVRGDILEIFPASNDEKAIRIEFFGDE
                     VDRITEIDYVTGKIVGTRNHVVIFPASHYVTTPERIEKAIVEIENELQEQIKFFKEND
                     RLLEAQRIEQRTKYDIEMLKEIGFCQGIENYSRHITGRSEGERPYTLMDFFPDDYLII
                     VDEAHVTIPQVRGMYAGDRSRKTSLIENGFRLPSALDNRPLNFQEFEGNINQMLFVSA
                     TPGPYEIQHSETIAEQIIRPTGLLDPIVEVRPINNQIDDLVGEITKTIEKNERVLITT
                     LTKKMSEDLTNYLKEIGIKVKYLHSDIVTLERTEIIRDLRLGKFDVLVGINLLREGLD
                     IPEVSLIAILDADKEGFLRSETALIQTIGRAARNENGRVIMYADRITDSMQNAIDETK
                     RRRDIQNLYNEEHNIIPKTIQKNIRDSIEATKVAEEEVVYGISDTDDKDEIRANIDKL
                     KSEMMEAAQNLQFERAAELRDKVKQLEEKLEK"
     gene            8312..11137
                     /gene="uvrA"
                     /locus_tag="PC700_001505"
     CDS             8312..11137
                     /gene="uvrA"
                     /locus_tag="PC700_001505"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF2580819.1"
                     /translation="MEDKIIIRGAKEHNLKNIDLELPRDKFIVFTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMEKPNVEYIEGLSPAISIDQKTTSKNPRSTVGTV
                     TEIYDYLRLLFARVGDVYCPTCGEKISQMTIQEIVDKMLDFPDRTKLQILSPIVRGQK
                     GTHKKVFDNIKKEGFVRVRVNGENYEVSDDIILNKNQKHNIEVVVDRIVIKDGIESRL
                     ADSIETAVKLSDGLVIAQIIDGEEILFSTKFACPEHGIGIEELSPRMFSFNAPFGACD
                     VCNGLGESKEVDPELVVPNKDLSIKQGAVAAWGSTGVNDDTYYSKMIKSLAEHFNVSL
                     TTPFKDLPDDFVEELLYGKDNIIVEFTYESKFGGTRNYKSYFEGVIVNLERRYRETNS
                     DYMRDKIEEYMAERPCPKCKGMRLKKEVLSVLVDGKNIMEVTNLSVNELIDFMENINL
                     TEKQRFIAHEIIKEIKGRAMFLRDVGLDYLNLSRKAGTLSGGEAQRIRLATQIGSALV
                     GVLYVLDEPSIGLHQRDNDRLITTLRHLTDLGNTLIVVEHDEDTMREADYVIDIGPGA
                     GIHGGEIVAQGTLDEIIENENSITGQYLSGKKQILLPETTREGNGSFIEIVKASENNL
                     KNIDVKFPLGKFTCITGVSGSGKSTLINDILYKGVASKVNRLKQRAGKHKEIKGIESI
                     DKVINIDQSPIGRTPRSNPATYTGVFDFIRDIFASTNEAKARGYKKGRFSFNVKGGRC
                     EACKGDGIIKIEMHFLPDVYVPCEICKGERYNRETLQVKYKDKTISDILDMNVEEAVE
                     FFENIPNIKRKLETLMDVGLSYIKLGQPSTQLSGGEAQRIKLAAELSKRPTGKTLYIL
                     DEPTTGLHMADVDKLIQVLQRLADTGNTIIVIEHNLDVIKTCDYVIDLGPEGGDKGGT
                     IVATGTPKEVSKVDGSYTGQFLKKYFS"
     gene            11396..13213
                     /gene="uvrC"
                     /locus_tag="PC700_001506"
     CDS             11396..13213
                     /gene="uvrC"
                     /locus_tag="PC700_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDF2580820.1"
                     /translation="MFDIQEHLKKLPSEPGVYLMKDKYDHIIYVGKAISLKNRVRQYF
                     QSSKNHTSKVKSMVKNIYKFEYIITDSELEALILECNLIKRYRPKYNVVLRDDKTYPY
                     IKVTTNEDYPRILKVRRVLKDKAKYFGPYTNITAVNDTLELISSTYPIRSCKIDIDKA
                     IKNKTRPCLNLHINKCLGPCTGNVSKEEYGKMIEEIIMCLSGKEEKLMELLKEKMNES
                     SMNFRFEEAAVYRDKIKSLEEMIQKQKIDATVSDLNQDVVAMARAHNEACVQVFFIRN
                     GKIVGREHFILEGVMDSPRASILSSFVKQFYNEQEYIPKELIIEDEIEDSSILEEWLS
                     SKKGQKVTIRVPQKGEKKSLVEMVRKNAVEYLEKFSDMNKRKYEKSIGALEELKQILN
                     LEKLPIRIEAYDISNIQGVDSIGSMVVYTNAKKDKKEYRRYKIKTVIGPNDYDSMAEI
                     VDRRLKHGNLPDLILLDGGKGQVSAVKKVLELNDVDIPLWGMYKDDKHRTKGLICKEK
                     EIELDKTTNLYRFIASIQEEVHNYAITYHRSLRNKALTKSILDDIQGIGEKRKKSLLN
                     HFKDVEAIKKATMEELLEVDGMNKSIADNVYNFFRKEEN"
     gene            13213..14142
                     /locus_tag="PC700_001507"
     CDS             13213..14142
                     /locus_tag="PC700_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HDF2580821.1"
                     /translation="METSNKVSIREIIKELKLKVVYMPDDVDYYVYSQDINRPGLQFA
                     GYFDYFAYERIQIVGKTEYNFFGLMDSHIREEVLDRFFSYEIPALIISRDLEIKPDVI
                     EKAKKYKRILLSTNRNTTRLINRLSNYLDNKLAPHTTIHGVLVDIYGIGVLIKGESSI
                     GKSETALELIQRGNRLVADDAVEIRKLDESMLVGQSPELIRHFLEIRGIGIIDVRSLY
                     GVGAIKNSKMIDLVVHLEAWDETKYYDRLGLDKEYEEILGVNVEKLVVPVKPGRNTAM
                     ILEVAAMNFRQRGMGYDAAQEFTKKLSKLIDTK"
     gene            14356..16152
                     /locus_tag="PC700_001508"
     CDS             14356..16152
                     /locus_tag="PC700_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate oxidoreductase"
                     /protein_id="HDF2580822.1"
                     /translation="MGIEYFSKLLESSTEKELLLSKKSNNISTARLISFLIVIAGFAI
                     GFYNKNIVGIFVGVLSIIIFIALLVIHSKVKEEETYFKSKSEVLNKYIKRFGDGWKEF
                     KIDGKEYLKDENSQAKDLDLFGRASLYQYICVANTSYGKNFLAKYLWNENPDENVILE
                     RQRAIKELLSKDDFSIHIQILSNIIGKEQKNNSDSSIESFIEYGENKNVYIPKWMHIF
                     TWGLPTATILSFIFCMLGFLPVLPVFLLFIIQLGFSGFGYPRLMQTLSPLFSFSRSIQ
                     VYEKMFEVLEKETFESAYLKELQDKLSKGSGVSRGVKQLNSIGNAVNLRYNQILYIIA
                     CGVLMWNYHCAEALERWKGIYGNQIRDWFESIGEFEALISLTVISHVKENTCFPVIKY
                     EDTPRLKVEEVYHPLIAEKSVIANSIQLNSQTCIITGSNMSGKTTFLRSIGVNLVLAY
                     AGAPVCAKSFDATCMAIFTSMRIQDDVSQGISTFYAEILRIKSMIQYSLKELPMLVLV
                     DEIFKGTNSADRIIGASEAVKKLSKPWVISMVTTHDFELCDLSGSGDVEVVNYHFSEY
                     YVDDKIHFDYKIKDGRCKTTNAKQLMRMAGIL"
     gene            complement(16292..18073)
                     /locus_tag="PC700_001509"
     CDS             complement(16292..18073)
                     /locus_tag="PC700_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HDF2580823.1"
                     /translation="MSLVNLTINGKHVSAPSGTSILDAAKLINIKIHNLCHLHMNEID
                     KLDTCASCRICMVETERGLVPACGTVIKEGMKVQTNSAKALNARRTIVELLLSDHPQD
                     CFVCEKNGDCELQTIAADLGVRKIRYQGSKSFAGKDTSTKSLVKDHSKCILCRRCETV
                     CNDIQTVGALSGVNRGFNTLVSTFFNSDMVETECTFCGQCISVCPTGALTEVDNVPKL
                     WDVLNKKEKTIVVQVAPAVRVAIGEEFGLEPGSISTGKMVAALKALGFEHVFDTNFGA
                     DFTIMEEATEFIERIQKGENLPILTSCCPAWVNFLEHNYPDKLNLASSCKSPQGMFGS
                     IAKNYYAPKILGINPEELYVVSVMPCVAKKYEASREELSESGILDVDLSITTRELAKM
                     IKEAAIDLPNLQDQDFDNPLGKSTGAASIFGASGGVLEAALRTSYEKITNKTLDNVNF
                     TNVRGLKGIREASIDVDGTTVNVCIVNTLKNARKIMDKVRSGECKYHIIEVMACPGGC
                     VGGAGQPYHHGNTEIVDRRANALYEIDRNKAIRKSHENPDLQAIYKDFFGEPNSDVAH
                     KYLHTHYFDKSCVYGECPQECACEEAK"
     gene            complement(18096..19982)
                     /locus_tag="PC700_001510"
     CDS             complement(18096..19982)
                     /locus_tag="PC700_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HDF2580824.1"
                     /translation="MRKVNSFDELKVLADELKPNLSLRKNYSKENVLRRELLVCCDTG
                     CTSSNSLEIVSELENEIKKSGIQDKVSVRLTGCFGFCAQGPIVKVYPDNVFYVKVEPS
                     DAEKIVQSHLIRNTVVEELLYEEESLSKKVEEQEEMSFYKKQLRIALKNCGLIDPDSI
                     EEYIANDGYLALGKCLTSLTPQEVIAEVKTSGLRGRGGAGFPTGSKWEAASKNPAGPT
                     DKKFVVCNADEGDPGAFMDRSVLEGDPHSVLEAMAICGYAIGSDTGYIYIRAEYPKSI
                     ERLKVAIAQAEKYGLLGENILGTGFNFKLELKYGAGAFVCGEGTALMHSIEGRRGEPR
                     MKTYSSSKSGLWKSPTCLNNVETFANIPAIILKGGDWFANLGTEDSSGTKVFALGGKV
                     ENVGLVEVPMGTTLRDIVFEIGGGIPEGKKFKAVQTGGPSGGCIPTEHLDTPIDFGSL
                     SSIGSMMGSGGMLVLDESDCMVDIAKFFLEFTVEESCGKCTPCRIGTTRLLEILTKIT
                     EGNGTLQDLDDLENLAETIQTASLCGLGKAAPNPVLSTLQYFKDEYIAHVVDKKCPAG
                     KCQNLLSYFITDDCKGCTKCSRVCPAGCITGSVKEQHTIDTSKCLKCGACIDNCTFNA
                     IIKK"
     gene            complement(19999..20496)
                     /locus_tag="PC700_001511"
     CDS             complement(19999..20496)
                     /locus_tag="PC700_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HDF2580825.1"
                     /translation="MCDFISHNKQLFDELDIFIDSLTTKEGALIQVLHEAQGIFGYLP
                     KEVQLHVARKLGVAPAKVYGVVTFYSYFTTEPVGKYKISVCLGTVCFVKGADKILSAF
                     EKQLGIKVGETTSDFKFSLEGLRCLGACGLAPVVTVNGKVYGKVKPDQVSEILDTYRE
                     LELNC"
BASE COUNT         7983 a         2751 c         3545 g         6395 t
ORIGIN      
        1 taactctaaa gaaactagta ttactaggtt ctataaactt attaaaaact taatagaaat
       61 attaagcata aagattatgt ggttacaata gcagagagga tacacctgtt cccattccga
      121 acacagaagt taagctctct agcgctgatg gtacttggtg ggaaactgcc tgggagagta
      181 ggacgtagcc acgtaatttt ttttgggtaa atttataaaa attaaaaaag ttttattttt
      241 tatgtttaaa ccttgtaaat ttgggacata atataattgt aaaacttaat atagcaaaaa
      301 taaattactc aatctccccc acttaaagac ctctatcccc catagaggtc ttttttattt
      361 tcaaaccaaa ttatataaaa aataaatagt tttatatgac tcttcataaa ttaaaatcac
      421 attgtgatag caaaccatga atatgaaaaa tatagaaaat tatagctaac ataagctgta
      481 tatcatctta acttcagtat atttaacaaa aaatgtttat aatttaacct ttattttaat
      541 aagtttacta tatattcact ttgtcttaat atatccttta caaattaaga gtattattaa
      601 tctataagta aatcgtagaa ttaaattaaa tattttggga ggataaaaat ggcagatata
      661 agactagaaa atgtaacaaa atcttataat aaaaaaaata cagttataga aaatttaaat
      721 cttactatag aagatggttc ttttacagta ttggtaggtc caagtggatg tggtaaatct
      781 actacattaa gaatgattgc aggtcttgaa gatataactt caggcaagtt aaaaattgaa
      841 gaaaatgacg ttacaaaaac ggatgctagt aaaagggata tagctatggt atttcaaaat
      901 tatgcattat atccacatat gactgttaga gaaaacatag aatttggtct taaaaacaaa
      961 aaagtagaaa aagaaaaaag agaaaaactt ataacagaag ttattgatgt agtaggattg
     1021 agagaatatt taaatgtgaa accaggcaac ttatctggag gtcaaagaca aagagtggca
     1081 cttgctagag ctatggttaa aaatccaaag gtatttttaa tggatgaacc attgtcaaat
     1141 ttagatgcta aattaagaaa tcaaatgaga acagaattaa tacaattaca tcaaaaacta
     1201 aaatctacat ttgtttatgt aactcatgac caggttgagg caatgtcaat ggcagataag
     1261 atagttataa tgaacaaagg aaagataatg caaataggtt ctccaaagaa gatatatcaa
     1321 aatcctaaga atttatttgt agctcaattt atagggagtc cttgtatgaa catactagat
     1381 ataaaaaatg atattaaatt tggattcaga ccagaaaaag caacactaga gagtataaac
     1441 atagatggaa aaaatgatga tgtatttagc ctatttggaa aagttgtgac taaagaagtg
     1501 ttgggaaatg aaactatata ttgtttatct gttcttaata gagaaataag agtcaaaacg
     1561 gaaaatgata tatttgatat tggaagaact ttaagaataa gtgtattaga aaaagattta
     1621 tattattttg ataaaaatgg agaaagaata gaagatgtaa aagtatgtaa aagtatgtat
     1681 tctaaaatta aaggagatat ttatgaaaaa gcaatttaat aaagcatatt tatatataac
     1741 acctgctatt ataggaactt tattgtttat agtctatcca ataattaaga cgatatctct
     1801 aagcttcaaa tctggtagtc ttcttagttc aacctttgaa aatgtaggct taaacaatta
     1861 tacagaactt ttaaagaatc cagagtttat acaaactata acgaacactg caatttatac
     1921 tttttttatg gtgactatat ctatatcact agccatagta cttgctgttt ggctaaataa
     1981 aaattctata atacacaatt taacgcaaag tataatcttt acaccacatg taatttcact
     2041 agtatctgta gctgtattgt ggctttggat tatggagcca caatatggct ttttaaactg
     2101 gatactatcg acattacatt taccaacatc aaagtggctt tcaagtgaaa gttcagcttt
     2161 aatgtcacta attctaatag gaatatggaa gactcttgga tacaatatct taatagtatt
     2221 atcaggtctt aaatccatac caaactatat atatgaagca tctaaattag ataatgcaaa
     2281 caaaataacg aatttcttta aagtgacatt gccgctatta tcaccaacaa tattttttct
     2341 tatgataaca acaacaactg catcttttca agtatttgat gagataagta taatgacaca
     2401 aggtgggcca gtgggttcaa ctaacatgtt atcttattat atatatgagt ctggatttat
     2461 atactatgac atagggcaag cgagtgcagc gagtatgttt ttgttagcta tagttatgat
     2521 tgctacatat gctaacttta gactcttatc aaagaaagtt cattatcaat aaagaaaaag
     2581 tagatattat aagatttatc caaaaggagg agacaatgga tattatacaa cctatcaaaa
     2641 aagacaaagt tgatataaaa aaaatagcat caaaatcaat ggaaatatta attctattac
     2701 ttttatctgc aatatttata ttccctttta tatggatgct atcgacatcc tttaaaaatc
     2761 cacaagaaat gatgcaatta ccacctacta ttataccaaa tgatatagtg ttagaaaatt
     2821 atagtacagc atggcattca ggtccatttt ttagatattt actaaacagt gtatttgtaa
     2881 caggaagtgt tttaatttta caatttttag ttatggttcc agcagcatat gcattttcta
     2941 agattaaatt taaaggtgaa aaaatgttat ttgggctttt gcttgtagga cttatgattc
     3001 caccacaagt aacttttcta ccagtctata tgatgatgag taagtttgga tttataaata
     3061 cttatattcc acttataatt ccatttatga catcttcatt tgggatattt ctgcttagac
     3121 agaattttat gcagatttca gatgaaacta ttgaagcagc taaactagat aatgcttctg
     3181 acttaaaaat aatgtttaga ataatggttc ctatggcaaa accagcagta ataacattta
     3241 tattatttaa ttttatatac cattggaata attatttctg gcccttagta atgacaaata
     3301 cagatgcaat aagaacttta ccaataggag ttgcgctcct taaatcttca gaaggaatta
     3361 ctgcatggaa tgtaataatg gcaggaaata taattctaat tttaccaata attcttgttt
     3421 atatatttgc gaataaaaaa gtaaaagaag cttttatgta ttcaggaata aaataaaaaa
     3481 ttaaagacta aatttggagg atgaaaatga agcttaaaag aaaattagca acaggattag
     3541 caataacatt attattaggt tctttagttg ggtgttcaaa ttctaatgat gcaaatgcat
     3601 ctaaaggaga aaagactgat agcaatatta cgaagataga tttctgggct cctttaggtg
     3661 gaactaatgg agaaactgct caaaaaatgg ttgaccaatt taatagtgaa cataaggatg
     3721 tacaagtaaa tatgttaaaa cagaaagatt actatgaaaa tgcaacaaaa ctacaggcag
     3781 ccttaacatc taaagaccaa ccagatgtaa cattattaga aataactcaa acaggtacat
     3841 tcgcatcagc aggtgcttta gtagatatga gtaaatattt tgataaaaca tatcaagaaa
     3901 gattcttttc tggtctttta acaaattcat attatgagga taagtttgtt ggtatgccat
     3961 ttaatagaag tacaccaata ttatacataa atagagatat ggcaactaaa gcaggtcttg
     4021 acccttcagg tccaaaatca tgggaagagc ttaaggatta cgctagtaaa atgacaaata
     4081 aatcagaaga tacttatggt tttgaaacac caatagatat ttggttctat gaagctatgg
     4141 taatgcaaag tggaggagaa ataactaatg gtaaaaaagt agcatttaat aatgaggcag
     4201 gacaagcacc agttaaattc tggcaagata tgatgaaagc tggaataatg aaaatgcctc
     4261 ctggagaaga ttataatgcc tgggacgtag caaaacaaga ttttgtaaat ggtaaagtag
     4321 gtatgatatt tacatcaaca gcagatttag caggacttat gcaacaaaca gaaggtaagt
     4381 ttgaaatatc aacagcattc ctaccaaaaa atcaaaaata cgcaactcct acaggtggag
     4441 ctaatctagt tatgcttgaa ggtggaacag ataaagaaaa agaagcaagt gcagaattta
     4501 tagaatggat gacacaaaca gataaaatcg ttcagtttag ttcatcaaca ggatacttac
     4561 caacaactga agatgcaaca aactcagaaa agttacaaac attatataaa gaaaaacctc
     4621 aatataaggt tgcaactgac caattacaat atgctgttgc acttccaatg ttaaatggat
     4681 acaaggaagc aactgataag ctaatggatg agattaaaaa ggggcttact gatttaaatg
     4741 caaatccaaa agatgtagta agcaaaggaa cttctgcaat gcaagcagta atagataaaa
     4801 caaataaata attatataga cttgtaagtt gattaaaagt attcaataat atataaatga
     4861 agaggtaatg taaatgacaa aggttatagt attatcagta gattctttat ttgaaaaaga
     4921 cttggaattt gtaaaaaatc taccaaactt taaatctata ctagaaaatt gctccatagt
     4981 aaaaaatata aattgtgttt atcccactct gacatatccg tgtcatacta gtatggttac
     5041 aggagtatat cctaaaaaac atggtatata tcataatgaa aaatttgacc caaataaaga
     5101 aaataaagac tggtactggt attcaaagga cataaaagta aaaactatta tagatgtagc
     5161 aaaagaaaat aatctaacta cctctagtgt attatggcct gtaatgggag caaatccaaa
     5221 tatagattat aatattgcag aaatatgggc tcctagtaga gaagatgacc caagagaaac
     5281 ttttgaacga agctccagta aggttataat ggacggtatt tacaatagac attgtcatta
     5341 tattgattgg aaattagaac ccaacatgga tttatttgga gtaaactgtt cagtagatat
     5401 aataaaggaa tataaaccag atttaatgct aatacattta gctacattgg accatacacg
     5461 acataattat ggcttattta atgacgaagt agataaagca ttaaaaatga atgataaatg
     5521 gctaggagat attattcagg ctacaaagga tgcaggtaca tacaaagata caaattttat
     5581 aatacttggg gaccatggtc atcttagagt tgataaagtt ataaatccaa atgtcttgct
     5641 taaatcaaat ggatttataa aagttgaaaa tggagaagtt aaggattttg atgcctatgt
     5701 aaattcttct ggaatatcag ctcaaataat agtaaataac ttagataggt taacagaatt
     5761 aagagaactg cttgaagaat ttaaagaaga attattcata gagaatattt ttaccaaaga
     5821 ggaagcatct aaccttggtc ttgaaggtga ttttgaaatg gtgattgaag gtcttgaagg
     5881 aatttcattt ggaaatgatt tcgaaggaag tattataaaa gattcagata taaaagatta
     5941 caagtttgct gttgcgacac atggtcattt acctactaaa ggtaatagac ctccatttat
     6001 agcttttgga ccaaatatta aaggtggtgt ggttatagaa gaaggagacc tgagagcaca
     6061 tgcagcaact atattaaaaa tgtttgattt aaaattagat ggagttgaaa aagaagcatt
     6121 tgattttatt atgtaagttg aataaagtat ttattttcca tgaattatag acttctattg
     6181 caaagataga agtctttttt tatgttttag atttaagtaa aatttaaatg ggtaaaatat
     6241 ttaaaataac ttaaacaatt ttataaaggt atgatataat aatacatgtc aggaactaat
     6301 gttcgaaaaa aggagtgata ctatggattt taaaataaaa tcagacttca aacctacagg
     6361 tgaccaacca gaagctatta aatcaatagt ggattctata aatagaaatg agaaattttc
     6421 gacattatta ggtgtaacgg gttcaggaaa aacattcaca atggcaaata tcattcaaca
     6481 agttaagaaa cctaccctaa taatggctca taataaaact ctagcagcac aactttacag
     6541 tgagtttaaa gaatttttcc cagacaatgc agttgaatac tttgtaagtt actatgatta
     6601 ttatcaacca gaagcatatg tagctcacag tgacacttat atagaaaaag atgcaagcat
     6661 aaatgatgaa atagataaac ttcgacattc tgctacagca tctattttag agagaagaga
     6721 tactataata atatcatctg tatcttgtat atatggactt ggtgacccaa aagactataa
     6781 agaacttatg ttatctataa gaccaggtat gcaaagagac agagatgatg taataaaaag
     6841 gttaatagag atacaatacg aaagaaatga cataaacttt acacgaggta cctttagagt
     6901 tagaggagat atactagaaa tattcccagc tagtaatgat gaaaaagcaa taagaataga
     6961 attttttggt gatgaggtag atagaataac agaaattgat tatgtaacag ggaagattgt
     7021 tggtactaga aatcatgttg ttatattccc agcatctcac tatgtaacaa cgccagaaag
     7081 aattgaaaag gcaattgtag aaatagaaaa cgagcttcaa gaacaaatta aatttttcaa
     7141 ggaaaatgat aggctattag aagcacaaag aattgaacaa agaactaagt atgatataga
     7201 aatgttaaag gaaataggat tttgtcaggg gatagagaac tattctagac atattacagg
     7261 aaggtcagaa ggagaaagac catatacttt aatggatttc ttcccagatg attatttgat
     7321 aattgtagat gaggcacatg tcactatacc acaagtaaga ggtatgtatg caggagacag
     7381 gtctagaaaa acatcactta tagagaatgg ttttagatta ccttctgcac ttgacaatag
     7441 accattaaat tttcaagaat ttgaaggaaa tataaatcaa atgttgtttg taagtgctac
     7501 accaggacct tatgaaattc aacactcaga gacaatagca gaacagataa taagaccaac
     7561 tggcctttta gacccaattg tagaagtaag acctataaat aatcaaatag atgatttggt
     7621 aggagaaata actaaaacta tagaaaaaaa tgaaagagtg cttataacca ctttaactaa
     7681 aaaaatgagt gaggacttga ctaactatct taaagaaata gggataaaag ttaaatattt
     7741 acactcagat atagttacat tagaaagaac agaaataata agagatttaa gattagggaa
     7801 gtttgatgtc cttgtaggaa taaacttact tagagaagga ttggatatac cagaagtttc
     7861 acttatagca attttagatg cagacaaaga aggtttctta cgttccgaaa cagctttaat
     7921 acagactatt ggacgtgcag ctagaaatga aaatggaaga gtaattatgt atgcagacag
     7981 gataactgac tcaatgcaaa atgctattga tgaaacaaag agaagaagag atatacaaaa
     8041 tttatataat gaagagcaca atataattcc taaaactata cagaaaaata taagagatag
     8101 tatagaagct actaaggttg cagaggaaga agttgtatat ggaatatctg atacagatga
     8161 taaagacgaa ataagagcta atatagataa attaaaatca gagatgatgg aagctgctca
     8221 aaacttacaa tttgaaagag cagcagaact tagagataaa gtgaaacaat tagaagaaaa
     8281 attggaaaaa tagaaaggta aaaacaacat tatggaagat aaaattataa taagaggagc
     8341 aaaagaacat aatttaaaaa atatagattt agaattacca agagataaat ttattgtttt
     8401 tacaggtttg tcagggtctg ggaagtcttc tttggcattt gatacaatat atgcagaagg
     8461 gcagagaaga tatgtagaaa gtctatctgc ttatgcaaga caatttttag gtcagatgga
     8521 aaagccaaat gtagaatata tagagggttt atcaccagct atctcaattg accagaagac
     8581 tacctcaaaa aacccacgtt ctacggttgg aacagtaaca gaaatatatg attatttgag
     8641 actattattt gcaagagtag gagatgtgta ttgccctaca tgtggtgaaa aaataagtca
     8701 gatgactatt caagaaatag ttgataaaat gctagatttt ccagatagaa cgaaattaca
     8761 aattctttca ccaattgtta gaggacaaaa aggtacacat aaaaaagtct ttgataatat
     8821 caaaaaagaa ggatttgtaa gagttagagt taatggtgag aactatgaag ttagtgatga
     8881 tataatctta aataaaaatc aaaaacataa tatagaagtt gtagtggaca gaatagtaat
     8941 taaagatgga atagaatcaa gactagcaga ttcaattgaa actgcagtta agctatctga
     9001 tggtcttgta atagcccaaa taatagatgg agaagaaatt cttttttcaa ctaaatttgc
     9061 atgcccagaa catggaattg gcatagaaga attatctcca aggatgtttt catttaatgc
     9121 tccctttggt gcttgtgatg tttgtaatgg tcttggggaa agtaaagaag ttgacccaga
     9181 attagtagta ccaaacaaag atttatcaat aaaacaaggt gcagtggcag catggggttc
     9241 aacaggtgtt aatgatgaca cttattacag taaaatgata aaaagtttag cagagcattt
     9301 taatgtatct ttgacaacac cgtttaaaga tttaccagat gattttgtgg aggaattact
     9361 ttatggtaaa gataatataa ttgtagagtt tacttatgag tcaaaatttg gaggaactag
     9421 aaattataaa tcatactttg aaggtgtaat agtaaactta gagagaagat atagagaaac
     9481 taattcagat tacatgagag ataagattga agaatacatg gcagagagac catgtccaaa
     9541 gtgtaaaggg atgagactta aaaaggaagt tttatctgtt ttagttgatg gaaaaaatat
     9601 aatggaagtt acaaacttat ctgtaaatga attgattgac tttatggaaa atataaatct
     9661 tacagaaaaa cagagattta tagctcatga gattataaaa gagataaaag gaagagctat
     9721 gttcttaaga gatgtaggtc ttgattattt aaatttatca agaaaagctg gaactttatc
     9781 aggaggagaa gcgcaacgta taagattagc tactcaaata ggttctgcat tggttggagt
     9841 tttatatgta cttgatgagc caagtatagg gcttcaccaa agagataatg ataggcttat
     9901 aactactctt agacatctaa cagatttagg aaatacctta atcgttgtag agcatgatga
     9961 agacacaatg agagaagctg attatgttat agacattggt ccaggtgcag gtatacatgg
    10021 tggtgaaata gttgcacagg gtactttaga tgaaataatt gaaaatgaga actcaataac
    10081 gggtcaatat ttaagtggta aaaaacaaat attgcttcct gagactacta gagaaggaaa
    10141 tggaagtttt atagaaatag ttaaagcatc agaaaataat ctgaaaaata ttgatgtaaa
    10201 gttcccatta ggtaagttta catgcataac aggtgtttca ggttctggaa aaagtacatt
    10261 aataaacgat atactttaca aaggtgttgc aagtaaagtg aatagactta agcaaagagc
    10321 tggtaagcat aaggagataa aaggaattga aagtatagat aaagttataa atatagacca
    10381 aagtcctatt ggaagaacac ctcgttcaaa tcctgctact tatacaggtg tatttgattt
    10441 tataagagat atttttgcat ctactaatga agctaaagca agaggttata aaaaaggtag
    10501 atttagtttt aatgtaaaag gtggtagatg tgaagcttgt aagggtgatg gaataataaa
    10561 aatagaaatg catttcttac cagatgtata tgttccttgt gaaatttgta aaggtgaaag
    10621 atacaataga gaaacattgc aagtaaaata caaagataaa actatatctg atatattaga
    10681 tatgaatgtg gaagaagctg ttgagttttt cgaaaatata ccaaatataa aaagaaaatt
    10741 agaaacttta atggatgtag gactttcata tattaaatta ggacagcctt caactcaatt
    10801 atcaggagga gaagcgcaac gtataaaatt agctgctgaa cttagtaaaa gaccaacagg
    10861 aaagactttg tatatattag atgaacctac tactggactt catatggcag atgtagacaa
    10921 gctaatccaa gtattacaaa gactagcaga tacaggaaat acaattatag tcatagaaca
    10981 taatttagat gttataaaga cttgtgatta tgtaatagac ttaggaccag aaggcggaga
    11041 taagggtgga actatagttg caactggaac accaaaagaa gtatctaaag ttgatggttc
    11101 ttatacaggt caattcttaa agaaatattt ttcttaagtt atataaattg tgaaaaattt
    11161 tagagcagat atacgataaa ctgttttgtt ataagtaaat tagtcaaata taagttgtaa
    11221 atagtaatac aaagtaaagt ccatattact cttcatggtt aaaattgttt tcaaatcaat
    11281 tatagaaggg aatatggact ttaacttgtc taaagcactg gaaattgtat aactttttaa
    11341 aattcatatt ttagggtata attttagtat aaaatttaat gttttggagg gcgagatgtt
    11401 tgatatacaa gagcatttaa aaaaattacc ttctgagccc ggagtatatt taatgaaaga
    11461 caaatacgac catataatat atgtgggaaa agccatatct ctaaaaaata gggtaaggca
    11521 gtattttcaa tcttcaaaaa accatacatc taaagtaaaa tctatggtta agaatatata
    11581 taagtttgag tatattataa cggattcaga gttagaggca ttaatacttg aatgtaattt
    11641 aataaaaaga tacagaccaa agtataatgt agtgcttaga gatgataaaa cttatccata
    11701 tataaaagta actacgaatg aagattatcc aagaatctta aaagttagaa gagttcttaa
    11761 agataaggca aagtattttg gaccctatac caatataact gctgtgaatg ataccttgga
    11821 gcttataagt agtacgtatc caataagaag ctgtaaaata gatatagata aagcaataaa
    11881 gaataagaca agaccttgtt taaatctaca cataaataaa tgtttaggac cttgtacagg
    11941 aaatgtttct aaagaagagt atggaaaaat gatagaagag attataatgt gtttatctgg
    12001 taaagaagaa aaattgatgg aacttttaaa ggaaaaaatg aatgagagtt ctatgaactt
    12061 tagatttgaa gaggctgctg tatataggga taagataaag agccttgaag agatgataca
    12121 gaaacaaaaa atagatgcta ctgtaagtga cttaaaccaa gatgtggttg caatggcaag
    12181 agctcataat gaagcttgtg tccaggtatt ttttataaga aatggtaaga ttgtaggtag
    12241 ggaacatttt atattagaag gtgtaatgga tagtccaaga gcttctatat taagttcatt
    12301 tgtaaaacaa ttttataatg aacaagagta tatacctaaa gaattaatta tagaagatga
    12361 gatagaagat agttctatat tagaagaatg gctgtcatct aaaaaggggc aaaaagtaac
    12421 aataagagtt cctcaaaagg gagagaagaa aagcttagtt gaaatggtta ggaaaaatgc
    12481 tgtagaatat cttgaaaaat tttctgatat gaataaaaga aaatatgaaa aaagtattgg
    12541 agcattggaa gaattaaaac aaatattaaa tctagaaaag ttgccaataa gaatagaggc
    12601 ttatgatata tcaaatatac aaggtgtaga ttcgattggt tctatggtag tgtatacgaa
    12661 tgctaaaaaa gataagaagg aatataggag atataaaata aagacagtta ttggacctaa
    12721 tgattatgat tctatggctg aaattgtaga tagaagatta aaacatggaa atttacctga
    12781 cttgatattg ttggatggtg gaaaaggaca agttagtgca gtaaaaaaag tactagaact
    12841 caatgatgta gatatacctc tttggggaat gtacaaagat gataagcata gaactaaagg
    12901 attgatatgc aaagaaaaag aaatagaact agataaaaca actaatttat atagatttat
    12961 tgctagtata caagaagaag ttcataatta tgcaataaca tatcatagaa gtctaagaaa
    13021 taaagctttg acaaaatcta tcttagatga tatacaagga ataggtgaaa aaagaaagaa
    13081 atcattatta aatcacttta aagatgtaga ggctataaaa aaagctacta tggaagaact
    13141 tttagaagta gatggaatga ataaaagcat tgcagataat gtttataatt tttttagaaa
    13201 ggaagaaaat taatggaaac tagcaataaa gtatcaataa gagagataat aaaagagttg
    13261 aagttaaaag tagtatacat gcctgatgat gtagattatt atgtctactc tcaagatata
    13321 aatagaccag gattacagtt tgcaggatat tttgattatt ttgcatatga aagaatacaa
    13381 atagttggaa agacagaata taattttttt ggattaatgg atagtcatat tcgagaagaa
    13441 gtactagata gatttttctc atatgaaata ccagcgttaa taatttctag agacttggaa
    13501 atcaagccag atgtaattga aaaggctaaa aaatataaaa gaatactttt gagcacaaat
    13561 agaaatacca ctagacttat aaatagactg tcaaactatt tggataataa gttagcacca
    13621 cacacaacaa ttcacggggt gctagtagat atttatggta taggtgtact gataaaaggt
    13681 gaaagtagta taggaaagtc ggaaacagca ttagaactga ttcaaagggg taatagatta
    13741 gttgcagatg atgctgtaga aataagaaaa ttagatgaaa gtatgcttgt gggtcagtca
    13801 cctgaactaa taagacattt tcttgagata agaggaatag gaataataga tgttagaagt
    13861 ttatatggag taggggcaat caaaaattct aaaatgatag atttagtagt tcatttagaa
    13921 gcttgggatg aaactaagta ttatgataga ctaggactgg ataaagaata cgaagaaata
    13981 cttggtgtta atgtagagaa attagttgta cctgttaaac ctggaagaaa tactgctatg
    14041 atattagaag tagcagcaat gaattttaga caaagaggaa tgggatacga tgcagctcaa
    14101 gaatttacta aaaaattatc aaaattaatc gatactaagt agtactgaat aatagtgaac
    14161 tacacatcat ttaattaaat attgattagg attatgtcct acaccttata aattaaaatc
    14221 aagttgtata tatataatgt ttattaataa gatatattat ataaggataa tttaaagtac
    14281 agtgttagat tataataata ctagattaat tttatatttg agtttcatta tataacaaga
    14341 aagttggaat tttttatggg aatagaatat tttagtaaac ttttagaaag tagtactgaa
    14401 aaagaattgc tcttaagtaa aaagtcaaat aacataagta cagcaagatt aatatctttt
    14461 ttgatagtga tagcagggtt tgctattgga ttttacaata aaaatatagt aggtattttt
    14521 gttggagttt tatcaataat aatatttata gcactcttag ttattcatag taaagtaaaa
    14581 gaagaagaaa catattttaa aagtaagagc gaagtattaa ataagtatat taagagattt
    14641 ggagatggtt ggaaggaatt taaaatagat ggtaaagaat acttaaaaga tgaaaattcg
    14701 caagcaaaag atttagactt atttggaaga gcatcattgt atcaatatat ctgtgtagct
    14761 aatacatctt atgggaaaaa ttttttagcc aaatatttgt ggaatgaaaa tccagatgag
    14821 aatgtaatct tagaaagaca gagggctata aaagaacttt tatcaaaaga tgatttttct
    14881 attcatattc aaatactcag taatataatt ggtaaagagc aaaaaaataa ttcagatagt
    14941 agtatagaat cgtttataga gtatggagaa aataagaatg tgtatatacc aaaatggatg
    15001 catattttta cttggggtct accaactgca acaatcttat cttttatatt ttgtatgtta
    15061 ggatttttac ctgttttacc tgttttttta ctttttataa tccagttagg gtttagtggt
    15121 tttggatatc caagacttat gcaaactctt tctccattat tttcatttag tagaagtata
    15181 caagtatatg aaaaaatgtt tgaggtttta gaaaaagaga cgtttgagag tgcatatttg
    15241 aaagaattac aagataaact ttctaaggga agtggagttt caagaggtgt taaacaatta
    15301 aattcaatag gaaatgctgt aaacctaagg tataatcaga tactatatat aattgcctgt
    15361 ggtgttttga tgtggaacta tcattgtgca gaagctttgg agcgatggaa aggtatatat
    15421 ggaaatcaaa ttagagattg gtttgaatct attggagaat ttgaagctct aataagttta
    15481 acagttattt ctcatgtaaa ggaaaataca tgttttccag taataaaata tgaagatact
    15541 ccaagattga aagttgaaga ggtataccac ccattaatag cagaaaaaag tgtcatagca
    15601 aattcgatac aattaaattc tcagacttgt attatcacag gttcaaatat gtctggaaag
    15661 acaacatttt taagaagtat cggtgtaaac ttagtgctgg catatgcagg tgctccagta
    15721 tgcgctaaga gttttgatgc gacctgtatg gctatattta cttctatgag aatacaagat
    15781 gatgtaagtc aaggaatatc aactttctat gctgagattc ttagaataaa atcaatgatt
    15841 caatatagtt taaaagaact gccaatgttg gttctagtag atgaaatttt taagggaact
    15901 aactctgctg atagaattat tggagcaagt gaggcagtaa agaaattatc aaaaccctgg
    15961 gttataagta tggtgacaac acatgacttt gagctatgtg acctttcagg tagtggagat
    16021 gttgaagttg taaactatca tttctcagag tactatgtag acgataaaat tcattttgac
    16081 tataagataa aagatggaag atgtaaaact acaaatgcaa aacaacttat gagaatggca
    16141 ggtattcttt aattaataga taggttaatt aaagaataag tctatttaga aatataattt
    16201 aatttgtgaa attaataagg attgtcttaa attacgtaaa agtattttaa gacaatcctt
    16261 ttttaagaat aaattttata ctttgttatg actacttagc ttcttcacaa gcacattctt
    16321 gtggacactc tccatagaca caactcttat caaagtaatg agtatgaagg tatttatggg
    16381 ctacatcact atttggttct ccaaagaagt ctttataaat agcttgtaaa tcaggattct
    16441 catgagattt acgaatagcc ttatttctat ctatttcata taacgcattt gcacgtctat
    16501 caactatttc agtattacca tggtgataag gttgaccagc acctccaaca catccaccag
    16561 gacatgccat aacttctatg atatggtatt tacattctcc acttctgacc ttatccataa
    16621 tctttctagc atttttaagt gtatttacaa tacacacatt aactgtagtt ccatcaacat
    16681 ctatacttgc ttccctaatt cctttaagac ctctaacatt agtgaagttt acattatcta
    16741 aagtcttatt agttatcttc tcatatgaag tacgtagggc agcttctaaa actccaccac
    16801 ttgctccaaa tatagatgca gcacctgtag attttcctag aggattatca aaatcttggt
    16861 cttgaagatt aggtaaatcg atagcagctt cttttatcat tttagctaac tctctagtag
    16921 ttatagaaag gtcaacatct agtattccag attcacttaa ttcttctctt gaagcttcat
    16981 attttttagc cacacaaggc attacagata caacataaag ttcttcagga ttgattccta
    17041 atattttagg tgcatagtag tttttagcta tagaaccaaa cataccttga ggagacttac
    17101 agctagatgc taagtttaac ttgtctggat aattgtgttc taagaagttt acccatgcag
    17161 gacaacaact tgttaatata ggaagatttt cacctttttg gattctttct ataaattcag
    17221 tagcttcttc cattatagta aaatctgcac caaagtttgt atcaaataca tgttcaaatc
    17281 caagtgcttt taaagcagct accatttttc ctgtagagat tgaccctggt tctaatccaa
    17341 actcttcacc aatagcaact cttacagcag gagcaacttg cactactata gtcttttctt
    17401 ttttatttaa aacatcccaa agtttaggta cattatctac ttctgttaat gctccagttg
    17461 gacagacaga tatacattga ccacagaatg tacactctgt ttcaaccata tcagagttaa
    17521 agaatgtact aactaaagta ttaaatccac gattaactcc agataatgct cctactgttt
    17581 gtatgtcatt acatacagtc tcacatcttc tacataatat acacttagaa tggtctttaa
    17641 ctaaagattt agtagaagta tctttacctg caaaagattt ggaaccttga tatcttattt
    17701 ttcttactcc taaatcagca gctatagtct gtaattcaca atctccattt ttttcacaga
    17761 caaaacaatc ttgtggatgg tctgataaca ataattcaac aatagttcta cgagcattta
    17821 atgctttagc actattagtc tgaactttca taccttcctt tataactgtt ccacaagctg
    17881 gaactaagcc tctctctgtc tcaaccatac aaatacgaca agaagcacaa gtgtcaagct
    17941 tatctatttc gttcatatgt aaatgacaaa ggttgtgtat ttttatattt attaatttcg
    18001 cagcatctaa gatagaagtt ccactaggtg ctgaaacgtg tttgccatta atagttaaat
    18061 taactaaact cattgttatt cctcctccaa ataagttatt tcttgattat agcgttaaat
    18121 gtacaattat ctatacaagc accacatttt agacacttag aagtatctat tgtatgttgt
    18181 tccttaacag aacctgttat acaaccagca ggacacactc tagaacattt tgtacaaccc
    18241 ttacagtcat ctgttataaa gtaagaaagt aagttctgac atttaccagc aggacatttc
    18301 ttatctacaa catgagctat atactcatct ttgaaatatt gaagagtact taaaacaggg
    18361 tttggagcag ctttaccaag accacataat gaagcagttt gtatagtttc agcaaggttt
    18421 tctaggtcat ctaaatcttg tagagtacca ttaccttctg taattttagt taatatttct
    18481 aataatcttg tagtacctat acgacaaggt gtacatttac cacaagattc ttcaacagta
    18541 aactcaagga agaacttggc tatatctacc atacagtcag attcatcaag aacaagcata
    18601 ccaccagagc ccatcattga accgatagaa cttaatgaac caaagtctat aggagtgtct
    18661 aaatgttcag ttggtataca tccaccagat ggtccacctg tttgaacagc cttaaatttc
    18721 ttaccttcag ggattccccc accaatttca aatacaatat ctcttaaagt agtacccatt
    18781 ggaacttcaa caagtcctac attttctact tttccaccaa gagcaaagac tttagtacca
    18841 ctagaatctt cagtacctaa gttggcaaac caatctccac ccttaagtat aattgcaggt
    18901 atgttagcaa aagtctcaac gttatttaag caagtaggag atttccatag accactctta
    18961 gaagaactat aagttttcat tctaggttca ccacgtctac cttcaattga atgcattagc
    19021 gctgtacctt caccacaaac gaaagcacct gcaccatatt ttaattctaa tttaaagtta
    19081 aatccagtgc ctaagatatt ttcgccaagt aaaccgtatt tttcagcttg agctatggct
    19141 acttttaatc tttctataga ttttggatat tctgctctta tgtatatgta tcctgtatca
    19201 gaacctatag cgtatccaca tattgccata gcttctagta cactatgagg gtcaccttca
    19261 agtacagatc gatccataaa tgcacctggg tcaccttcat cagcattaca tacaacaaac
    19321 tttttatctg ttggaccagc tggatttttt gaagcagctt cccatttaga gccagtaggg
    19381 aaacctgcac cacctctacc acgaaggcca gaagttttta cctctgcaat aacttcttga
    19441 ggagtaagag aagttaaaca tttgcctaga gctaaataac catcattggc aatatattct
    19501 tctatactat ctgggtctat aagaccacaa ttttttaaag ctatacgtaa ctgttttttg
    19561 tagaaagaca tttcttcttg ttcttcaact tttttactta aagattcttc ctcatataat
    19621 aattcctcga caactgtatt tctaattaag tggctttgaa ctatcttttc agcatcagat
    19681 ggctcaactt ttacgtagaa aacgttatca ggataaactt ttacaatagg accttgagca
    19741 cagaaaccaa aacaacctgt caaacgaaca ctaactttat cttggattcc tgatttcttt
    19801 atttcatttt ctaattcact aactatttct agactgtttg aagaggtaca tccagtatca
    19861 caacaaacaa gtaattctct tcttaataca ttttccttag aataattttt tcttaagcta
    19921 agattaggtt tcagctcatc agctaaaact tttaattcat caaatgaatt tactttacgc
    19981 attatttatt cacccctctt aacaattcaa ttctaattct ctataagtat ctaatatttc
    20041 gcttacttgg tcaggtttca ctttaccata aactttacca tttacagtaa caactggagc
    20101 taaaccacat gcccccaaac atcttaaacc ctctaaagaa aatttaaaat cactagtagt
    20161 ttctccaact tttatgccaa gctgtttttc aaaagcactt aagattttgt cagcaccttt
    20221 tacaaaacat acagttccta gacagacact gattttatac ttacctacag gttctgtagt
    20281 aaaataagaa tagaatgtaa caaccccata tacctttgca ggagctactc caagttttct
    20341 agcaacatga agttgaactt cttttggaag atatccaaat ataccttggg cttcatgtag
    20401 aacttgtatt aacgcacctt cctttgttgt aagagagtca ataaatatat ctagttcgtc
    20461 aaataattgc ttgttgtgtg aaataaaatc gcacataaca aatccccctt ttctttttaa
    20521 agttattatt tttcataaaa taattaacca tgagttgacc ccaacattat gtgggtgaac
    20581 aaaaccagaa caataaagaa agaataatat acaacaattt ctttatttat aaaaacaatg
    20641 caaaaaagtt acctattaaa aataaaaact atac
//
LOCUS       DAMJWP010000048        20540 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.049, whole genome shotgun sequence.
ACCESSION   DAMJWP010000048 DAMJWP010000000
VERSION     DAMJWP010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20540
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.049"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            62..616
                     /gene="tcdR"
                     /locus_tag="PC700_001512"
     CDS             62..616
                     /gene="tcdR"
                     /locus_tag="PC700_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin sigma factor TcdR"
                     /protein_id="HDF2580826.1"
                     /translation="MQKSFYELIVLARNNSVDDLQEILFMFKPLVKKLSRVLHYEEGE
                     TDLIIFFIELIKNIKLSSFSEKSDAIIVKYIHKSLLNKTFELSRRYSKMKFNFVEFDE
                     NILNMKNNYQSKSVFEEDICFFEYILKELSGIQRKVIFYKYLKGYSDREISVKLKISR
                     QAVNKAKNRAFKKIKKDYENYFNL"
     gene            947..8047
                     /gene="tcdB"
                     /locus_tag="PC700_001513"
                     /gene_synonym="toxB"
     CDS             947..8047
                     /gene="tcdB"
                     /locus_tag="PC700_001513"
                     /gene_synonym="toxB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdB"
                     /protein_id="HDF2580827.1"
                     /translation="MSLVNRKQLEKMANVRFRVQEDEYVAILDALEEYHNMSENTVVE
                     KYLKLKDINSLTDIYIDTYKKSGRNKALKKFKEYLVTEVLELKNNNLTPVEKNLHFVW
                     IGGQINDTAINYINQWKDVNSDYNVNVFYDSNAFLINTLKKTIVESATNDTLESFREN
                     LNDPRFDYNKFYRKRMEIIYDKQKNFINYYKTQREENPDLIIDDIVKIYLSNEYSKDI
                     DELNSYIEESLNKVTENSGNDVRNFEEFKGGESFKLYEQELVERWNLAAASDILRISA
                     LKEVGGVYLDVDMLPGIQPDLFESIEKPSSVTVDFWEMVKLEAIMKYKEYIPGYTSEH
                     FDMLDEEVQSSFESVLASKSDKSEIFSSLGDMEASPLEVKIAFNSKGIINQGLISVKD
                     SYCSNLIVKQIENRYKILNNSLNPAISEDNDFNTTTNAFIDSIMAEANADNGRFMMEL
                     GKYLRVGFFPDVKTTINLSGPEAYAAAYQDLLMFKEGSMNIHLIEADLRNFEISKTNI
                     SQSTEQEMASLWSFDDARAKAQFEEYKKNYFEGSLGEDDNLDFSQNTVVDKEYLLEKI
                     SSLARSSERGYIHYIVQLQGDKISYEAACNLFAKTPYDSVLFQKNIEDSEIAYYYNPG
                     DGEIQEIDKYKIPSIISDRPKIKLTFIGHGKDEFNTDIFAGLDVDSLSTEIETAIDLA
                     KEDISPKSIEINLLGCNMFSYSVNVEETYPGKLLLRVKDKVSELMPSISQDSIIVSAN
                     QYEVRINSEGRRELLDHSGEWINKEESIIKDISSKEYISFNPKENKIIVKSKNLPELS
                     TLLQEIRNNSNSSDIELEEKVMLAECEINVISNIDTQVVEGRIEEAKSLTSDSINYIK
                     NEFKLIESISDALYDLKQQNELEESHFISFEDILETDEGFSIRFIDKETGESIFVETE
                     KAIFSEYANHITEEISKIKGTIFDTVNGKLVKKVNLDATHEVNTLNAAFFIQSLIEYN
                     SSKESLSNLSVAMKVQVYAQLFSTGLNTITDAAKVVELVSTALDETIDLLPTLSEGLP
                     VIATIIDGVSLGAAIKELSETSDPLLRQEIEAKIGIMAVNLTAATTAIITSSLGIASG
                     FSILLVPLAGISAGIPSLVNNELILRDKATKVVDYFSHISLAESEGAFTSLDDKIMMP
                     QDDLVISEIDFNNNSITLGKCEIWRMEGGSGHTVTDDIDHFFSAPSITYREPHLSIYD
                     VLEVQKEELDLSKDLMVLPNAPNRVFAWETGWTPGLRSLENDGTKLLDRIRDNYEGEF
                     YWRYFAFIADALITTLKPRYEDTNIRINLDSNTRSFIVPVITTEYIREKLSYSFYGSG
                     GTYALSLSQYNMNINIELNENDTWVIDVDNVVRDVTIESDKIKKGDLIENILSKLSIE
                     DNKIILDNHEINFSGTLNGGNGFVSLTFSILEGINAVIEVDLLSKSYKVLISGELKTL
                     MANSNSVQQKIDYIGLNSELQKNIPYSFMDDKGKENGFINCSTKEGLFVSELSDVVLI
                     SKVYMDNSKPLFGYCSNDLKDVKVITKDDVIILTGYYLKDDIKISLSFTIQDENTIKL
                     NGVYLDENGVAEILKFMNKKGSTNTSDSLMSFLESMNIKSIFINSLQSNTKLILDTNF
                     IISGTTSIGQFEFICDKDNNIQPYFIKFNTLETKYTLYVGNRQNMIVEPNYDLDDSGD
                     ISSTVINFSQKYLYGIDSCVNKVIISPNIYTDEINITPIYEANNTYPEVIVLDTNYIS
                     EKINININDLSIRYVWSNDGSDFILMSTDEENKVSQVKIRFTNVFKGNTISDKISFNF
                     SDKQDVSINKVISTFTPSYYVEGLLNYDLGLISLYNEKFYINNFGMMVSGLVYINDSL
                     YYFKPPIKNLITGFTTIGDDKYYFNPDNGGAASVGETIIDGKNYYFSQNGVLQTGVFS
                     TEDGFKYFAPADTLDENLEGEAIDFTGKLTIDENVYYFGDNYRAAIEWQTLDDEVYYF
                     STDTGRAFKGLNQIGDDKFYFNSDGIMQKGFVNINDKTFYFDDSGVMKSGYTEIDGKY
                     FYFAENGEMQIGVFNTADGFKYFAHHDEDLGNEEGEALSYSGILNFNNKIYYFDDSFT
                     AVVGWKDLEDGSKYYFDEDTAEAYIGISIINDGKYYFNDSGIMQIGFVTINNEVFYFS
                     DSGIVESGMQNIDDNYFYIDENGLVQIGVFDTSDGYKYFAPANTVNDNIYGQAVEYSG
                     LVRVGEDVYYFGETYTIETGWIYDMENESDKYYFDPETKKAYKGINVIDDIKYYFDEN
                     GIMRTGLITFEDNHYYFNEDGIMQYGYLNIEDKTFYFSEDGIMQIGVFNTPDGFKYFA
                     HQNTLDENFEGESINYTGWLDLDEKRYYFTDEYIAATGSVIIDGEEYYFDPDTAQLVI
                     SE"
     gene            8169..8669
                     /gene="tcdE"
                     /locus_tag="PC700_001514"
     CDS             8169..8669
                     /gene="tcdE"
                     /locus_tag="PC700_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like glycosylating toxin export protein
                     TcdE"
                     /protein_id="HDF2580828.1"
                     /translation="MHSSSPFYISNGNKIFFYINLGGVMNMTISFLSEHIFIKLVILT
                     ISFDTLLGCLSAIKSRKFNSSFGIDGGIRKVAMIACIFFLSVVDILTKFNFLFMLPQD
                     CINFLRLKHLGISEFFSILFILYESVSILKNMCLCGLPVPKRLKEKIAVLLDAMTDEM
                     NAKDEK"
     gene            8748..8909
                     /locus_tag="PC700_001515"
     CDS             8748..8909
                     /locus_tag="PC700_001515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580829.1"
                     /translation="MKLIMKTREQMPRDTQVLNTYNFEASVHYYMDDKVVYQTLVHKD
                     GAWSVGKIY"
     gene            8998..9180
                     /locus_tag="PC700_001516"
     CDS             8998..9180
                     /locus_tag="PC700_001516"
                     /inference="COORDINATES: protein motif:HMM:NF022413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580830.1"
                     /translation="MRDEGQNDRENKYQTLINDLIDSLKVIGKIYKEIKTLKKYKICL
                     QITIRQSPYLIEESIN"
     gene            9397..17529
                     /gene="tcdA"
                     /locus_tag="PC700_001517"
                     /gene_synonym="toxA"
     CDS             9397..17529
                     /gene="tcdA"
                     /locus_tag="PC700_001517"
                     /gene_synonym="toxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021394011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdA"
                     /protein_id="HDF2580831.1"
                     /translation="MSLISKEELIKLAYSIRPRENEYKTILTNLDEYNKLTTNNNENK
                     YLQLKKLNESIDVFMNKYKNSSRNRALSNLKKDILKEVILIKNSNTSPVEKNLHFVWI
                     GGEVSDIALEYIKQWADINAEYNIKLWYDSEAFLVNTLKKAIVESSTTEALQLLEEEI
                     QNPQFDNMKFYKKRMEFIYDRQKRFINYYKSQINKPTVPTIDDIIKSHLVSEYNRDET
                     LLESYRTNSLRKINSNHGIDIRANSLFTEQELLNIYSQELLNRGNLAAASDIVRLLAL
                     KNFGGVYLDVDMLPGIHSDLFKTIPRPSSIGLDRWEMIKLEAIMKYKKYINNYTSENF
                     DKLDQQLKDNFKLIIESKSEKSEIFSKLENLNVSDLEIKIAFALGSVINQALISKQGS
                     YLTNLVIEQVKNRYQFLNQHLNPAIESDNNFTDTTKIFHDSLFNSATAENSMFLTKIA
                     PYLQVGFMPEARSTISLSGPGAYASAYYDFINLQENTIEKTLKASDLIEFKFPENNLS
                     QLTEQEINSLWSFDQASAKYQFEKYVRDYTGGSLSEDNGVDFNKNTALDKNYLLNNKI
                     PSNNVEEAGSKNYVHYIIQLQGDDISYEATCNLFSKNPKNSIIIQRNMNESAKSYFLS
                     DDGESILELNKYRIPERLKNKEKVKVTFIGHGKDEFNTSEFARLSVDSLSNEISSFLD
                     TIKLDISPKNVEVNLLGCNMFSYDFNVEETYPGKLLLSIMDKITSTLPDVNKDSITIG
                     ANQYEVRINSEGRKELLAHSGKWINKEEAIMSDLSSKEYIFFDSIDNKLKAKSKNIPG
                     LASISEDIKTLLLDASVSPDTKFILNNLKLNIESSIGDYIYYEKLEPVKNIIHNSIDD
                     LIDEFNLLENVSDELYELKKLNNLDEKYLISFEDISKNNSTYSVRFINKSNGESVYVE
                     TEKEIFSKYSEHITKEISTIKNSIITDVNGNLLDNIQLDHTSQVNTLNAAFFIQSLID
                     YSSNKDVLNDLSTSVKVQLYAQLFSTGLNTIYDSIQLVNLISNAVNDTINVLPTITEG
                     IPIVSTILDGINLGAAIKELLDEHDPLLKKELEAKVGVLAINMSLSIAATVASIVGIG
                     AEVTIFLLPIAGISAGIPSLVNNELILHDKATSVVNYFNHLSESKEYGPLKTEDDKIL
                     VPIDDLVISEIDFNNNSIKLGTCNILAMEGGSGHTVTGNIDHFFSSPYISSHIPSLSV
                     YSAIGIKTENLDFSKKIMMLPNAPSRVFWWETGAVPGLRSLENNGTKLLDSIRDLYPG
                     KFYWRFYAFFDYAITTLKPVYEDTNTKIKLDKDTRNFIMPTITTDEIRNKLSYSFDGA
                     GGTYSLLLSSYPISMNINLSKDDLWIFNIDNEVREISIENGTIKKGNLIEDVLSKIDI
                     NKNKLIIGNQTIDFSGDIDNKDRYIFLTCELDDKISLIIEINLVAKSYSLLLSGDKNY
                     LISNLSNTIEKINTLGLDSKNIAYNYTDESNNKYFGAISKTSQKSIIHYKKDSKNILE
                     FYNGSTLEFNSKDFIAEDINVFMKDDINTITGKYYVDNNTDKSIDFSISLVSKNQVKV
                     NGLYLNESVYSSYLDFVKNSDGHHNTSNFMNLFLNNISFWKLFGFENINFVIDKYFTL
                     VGKTNLGYVEFICDNNKNIDIYFGEWKTSSSKSTIFSGNGRNVVVEPIYNPDTGEDIS
                     TSLDFSYEPLYGIDRYINKVLIAPDLYTSLININTNYYSNEYYPEIIVLNPNTFHKKV
                     NINLDSSSFEYKWSTEGSDFILVRYLEESNKKILQKIRIKGILSNTQSFNKMSIDFKD
                     IKKLSLGYIMSNFKSFNSENELDRDHLGFKIIDNKTYYYDEDSKLVKGLININNSLFY
                     FDPIESNLVTGWQTINGKKYYFDINTGAASTSYKIINGKHFYFNNNGVMQLGVFKGPD
                     GFEYFAPANTQNNNIEGQAIVYQSKFLTLNGKKYYFDNDSKAVTGWRIINNEKYYFNP
                     NNAIAAVGLQVIDNNKYYFNPDTAIISKGWQTVNGSRYYFDTDTAIAFNGYKTIDGKH
                     FYFDSDCVVKIGVFSGSNGFEYFAPANTYNNNIEGQAIVYQSKFLTLNGKKYYFDNNS
                     KAVTGWQTIDSKKYYFNTNTAEAATGWQTIDGKKYYFNTNTAEAATGWQTIDGKKYYF
                     NTNTSIASTGYTIINGKYFYFNTDGIMQIGVFKVPNGFEYFAPANTHNNNIEGQAILY
                     QNKFLTLNGKKYYFGSDSKAITGWQTIDGKKYYFNPNNAIAATHLCTINNDKYYFSYD
                     GILQNGYITIERNNFYFDANNESKMVTGVFKGPNGFEYFAPANTHNNNIEGQAIVYQN
                     KFLTLNGKKYYFDNDSKAVTGWQTIDSKKYYFNLNTAVAVTGWQTIDGEKYYFNLNTA
                     EAATGWQTIDGKRYYFNTNTYIASTGYTIINGKHFYFNTDGIMQIGVFKGPDGFEYFA
                     PANTHNNNIEGQAILYQNKFLTLNGKKYYFGSDSKAVTGLRTIDGKKYYFNTNTAVAV
                     TGWQTINGKKYYFNTNTYIASTGYTIISGKHFYFNTDGIMQIGVFKGPDGFEYFAPAN
                     TDANNIEGQAIRYQNRFLYLHDNIYYFGNDSKAATGWATIDGNRYYFEPNTAMGANGY
                     KTIDNKNFYFRNGLPQIGVFKGPNGFEYFAPANTDANNIDGQAIRYQNRFLHLLGKIY
                     YFGNNSKAVTGWQTINSKVYYFMPDTAMAAAGGLFEIDGVIYFFGVDGVKAPGIYG"
     gene            complement(17864..18424)
                     /locus_tag="PC700_001518"
     CDS             complement(17864..18424)
                     /locus_tag="PC700_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin regulator"
                     /protein_id="HDF2580832.1"
                     /translation="MFFGNISSPACSEDHEEVISNQTSVIDSQKTEIETLNSKLSDAE
                     PWFKMKDDEKKAIEAENQRKAEEAKKAEEQRKKEEEEKKGYDTGITYDQLARTPDDYK
                     YKKVKFEGKVIQVIEDGDEVQIRLAVSGNYDKVVLCSYKKSITPSRVLEDDYITIRGI
                     SAGTITYESTMGGKITIPGIAVEKIN"
     gene            18961..19206
                     /locus_tag="PC700_001519"
     CDS             18961..19206
                     /locus_tag="PC700_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pathogenicity locus"
                     /protein_id="HDF2580833.1"
                     /translation="MGELQKIPGVGKATEKSLIMLGYTTIKSLKDANPAQMYEKECLM
                     RGQHIDRCQLYVYRCAVYFASTENPEPEKLKWWYWKD"
     gene            complement(19423..19692)
                     /locus_tag="PC700_001520"
     CDS             complement(19423..19692)
                     /locus_tag="PC700_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HDF2580834.1"
                     /translation="MFESRCGVCCDSCTKKEQVNCTGCPTMEKPFWGGECKVKTCCES
                     KELNHCGECDTFPCDMLLNKGKDQGFDPMVNIGQCRKWLEETVSN"
     gene            complement(19904..20206)
                     /locus_tag="PC700_001521"
     CDS             complement(19904..20206)
                     /locus_tag="PC700_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580835.1"
                     /translation="MKYSKGFKLIYSLVEYSKKFKVIYSVMKYSKGFKLIYSLVEYSK
                     RFKLIYDVMKYSKEFKLIYSLVEYSKRFKVIYSVMKYSKRFKAIYNAVKYIQGDLK"
BASE COUNT         8136 a         2354 c         3095 g         6955 t
ORIGIN      
        1 ttccaatgtt gtcaaaattt ttaaataaat catcattata aatataagag aggatgattt
       61 tatgcaaaag tctttttatg aattaattgt tttagcaaga aataactcag tagatgattt
      121 gcaagaaatt ttatttatgt ttaagccatt agtaaaaaaa cttagtagag ttttacatta
      181 tgaagaggga gaaacagatt taataatatt ttttattgaa ttaataaaaa atattaaatt
      241 aagtagcttt tcagaaaaaa gcgatgctat tatagtcaaa tatattcata aatcattact
      301 gaataagact tttgagttgt ctagaagata ttctaaaatg aagtttaatt ttgtagaatt
      361 tgatgaaaat atcttaaata tgaaaaataa ttatcaaagt aagtctgttt ttgaggaaga
      421 tatttgtttt ttcgaatata ttttgaaaga attatctggt attcaaagaa aagttatttt
      481 ttataaatat ttaaaaggat attctgatag agaaatatca gtgaaattaa aaatatctag
      541 acaagctgtt aataaggcta aaaatagagc atttaaaaaa ataaaaaaag actatgaaaa
      601 ttattttaac ttgtaattaa tgaatttaaa gaaatattta caatagaaat caaattttag
      661 aattaacttt attgtaaaat caataactta atctaagaat atcttaattt ttatatttta
      721 tatagaacaa agtttacata tttatttcag acaacgtctt tattcaatcg aagagcaaat
      781 taatcaactg agtgtcttca atttaaaatg ttaggaagtg aatgtatatg aaaacctaag
      841 tagatattag tatattttat aaatagaaag gaggatatat aaaagagttt tagcatttag
      901 atgtaaaaat attcaataaa aatattatag taaaggagaa aattttatga gtttagttaa
      961 tagaaaacag ttagaaaaaa tggcaaatgt aagatttcgt gttcaggaag atgaatatgt
     1021 agcaatattg gatgctttag aagaatatca taatatgtca gagaatactg tagtcgaaaa
     1081 atatttaaaa ttaaaagata taaatagttt aacagatatt tatatagata catataaaaa
     1141 gtctggtaga aataaagcct taaaaaaatt taaggaatac ctagttacag aagtattaga
     1201 gctaaagaat aataatttaa ctccagttga gaaaaattta cattttgttt ggattggagg
     1261 tcaaataaat gacactgcta ttaattatat aaatcaatgg aaagatgtaa atagtgatta
     1321 taatgttaat gttttttatg atagtaatgc atttttgata aacacattga aaaaaactat
     1381 agtagaatca gcaacaaatg atacacttga atcatttaga gaaaacttaa atgaccctag
     1441 atttgactat aataaatttt acagaaaacg tatggagata atctatgata agcagaaaaa
     1501 tttcataaat tactataaaa ctcaaagaga agaaaatcct gaccttataa ttgatgatat
     1561 tgtaaagata tatctttcaa acgagtattc aaaggatata gatgaactta attcctatat
     1621 tgaagagtca ttaaataaag ttacagaaaa tagtgggaat gatgttagaa actttgaaga
     1681 atttaaaggt ggagagtcat tcaaattata tgaacaagag ttggtagaaa gatggaattt
     1741 ggcagctgct tctgacatat taagaatatc tgccttaaaa gaagttggtg gtgtatattt
     1801 agatgttgat atgttaccag gaatacaacc agacctattt gagtctatag agaaacctag
     1861 ttcagtaaca gtggattttt gggaaatggt aaaattagaa gctataatga aatacaaaga
     1921 atacatacca ggatatactt cagagcattt tgatatgttg gatgaagaag ttcaaagtag
     1981 ttttgaatct gttctagctt ctaagtcaga taagtcagaa atattctcat cacttggcga
     2041 tatggaggca tcaccactag aagttaaaat tgcatttaat agtaaaggta ttataaatca
     2101 aggactaatt tctgtgaaag actcatattg tagcaattta atagtaaaac aaatcgagaa
     2161 cagatataag atattgaata atagtttaaa tccagctatt agcgaggata acgatttcaa
     2221 tactacaacg aatgctttta ttgatagtat aatggctgaa gctaatgcag ataatggtag
     2281 atttatgatg gaactaggaa agtatttaag agttggtttc ttcccagatg ttaaaactac
     2341 tattaactta agtggccctg aagcatatgc ggcagcttat caagatttat taatgtttaa
     2401 agaaggtagt atgaatatcc atttgataga agctgactta agaaactttg aaatctctaa
     2461 aactaatatt tctcaatcaa ctgaacaaga aatggctagc ttatggtcat ttgacgatgc
     2521 aagagctaaa gctcaatttg aagaatacaa aaaaaattac tttgaaggtt ctcttggaga
     2581 agatgacaat cttgactttt ctcaaaatac agtagttgac aaggagtatc ttttagaaaa
     2641 aatatcttca ttagcaagaa gttcagagag aggatatata cactatattg ttcagttaca
     2701 aggagataaa attagttatg aagcagcatg taacttattt gcaaagactc cttatgatag
     2761 tgtactgttt cagaaaaata tagaagattc agaaattgca tattattata atcctggaga
     2821 tggtgaaata caagaaatag acaagtataa aattccaagt ataatttctg atagacctaa
     2881 gattaaatta acatttattg gtcatggtaa agatgaattt aatactgata tatttgcagg
     2941 tcttgatgta gattcattat ccacagaaat agaaacagca atagatttag ctaaagagga
     3001 tatttctcct aagtcaatag aaataaactt actgggatgt aacatgttta gctattctgt
     3061 aaatgtagag gagacttatc ctgggaaatt attacttaga gttaaagata aagtatcaga
     3121 attaatgcca tctataagtc aagactctat tatagtaagt gcaaatcaat atgaagttag
     3181 aataaatagt gaaggaagaa gagaattatt ggatcattct ggtgaatgga taaataaaga
     3241 agaaagtatt ataaaggata tttcatcaaa agaatatata tcatttaatc ctaaagaaaa
     3301 taaaattata gtaaaatcta aaaatttacc tgaattatct acattattac aagaaattag
     3361 aaacaattct aattcaagtg atattgaact agaagaaaaa gtaatgttag cagaatgtga
     3421 gataaatgtt atttcaaata tagatacaca agtggtagaa ggaaggattg aagaagctaa
     3481 aagcttaact tctgactcta ttaattatat aaagaatgaa tttaaactaa tagaatctat
     3541 ttctgatgca ctatacgatt taaaacaaca gaatgaatta gaagagtctc attttatatc
     3601 ttttgaggat atattggaga ctgatgaagg ctttagtata agatttattg ataaagaaac
     3661 tggagaatct atatttgtag aaactgaaaa ggcaatattc tctgaatatg ctaatcatat
     3721 aactgaagag atttctaaga taaaaggtac tatatttgat actgtaaatg gtaagttagt
     3781 aaaaaaagta aatttagatg ctacacatga agtgaatact ttaaatgctg cattttttat
     3841 acaatcatta atagaatata atagttctaa agaatctctt agtaatttaa gtgtagcaat
     3901 gaaagttcaa gtttatgctc aattatttag tactggttta aatactatta cagatgcagc
     3961 caaagttgtt gaattagtat caactgcatt agatgaaact atagatttac ttcctacatt
     4021 atctgaagga ttacctgtaa ttgcaactat catagatggt gtaagtttag gtgcagcaat
     4081 caaagagcta agtgaaacaa gtgacccatt attaagacaa gaaatagaag ctaagatagg
     4141 tataatggca gtaaatttaa cagcagctac aactgcaatc attacttcat ctttaggaat
     4201 agctagtgga tttagtatac ttttagttcc tttagcagga atttcagcag gtataccaag
     4261 tttagtaaac aatgagctta tcctaagaga taaagcaaca aaagttgtag attattttag
     4321 tcatatatca ttagctgagt ctgaaggagc atttacttca ttagatgata aaataatgat
     4381 gccacaagat gatttagtaa tatctgagat agactttaat aacaattcaa taactttagg
     4441 taaatgtgaa atctggagaa tggaaggtgg ctcaggtcat actgtaactg atgatataga
     4501 tcacttcttt tcagcaccat caataacata tagagagcca cacttatcta tatatgacgt
     4561 attggaagta caaaaagaag aacttgattt gtcaaaagat ttaatggtat tacctaatgc
     4621 tccaaataga gtatttgctt gggaaacagg atggacacca ggtttaagaa gcttagaaaa
     4681 tgatggcaca aaactgttag accgtataag agataactat gaaggtgagt tttattggag
     4741 atattttgct tttatagctg atgctttaat aacaacatta aaaccaagat atgaagatac
     4801 taatataaga ataaatttag atagtaatac tagaagcttt atagtcccag tgataactac
     4861 agaatatata agagagaaat tatcatattc tttttatggt tctggaggaa cttatgcatt
     4921 atctctttct caatacaata tgaatataaa catagaatta aatgaaaatg atacttgggt
     4981 tatagatgtc gacaatgtcg taagagatgt cactatagaa tctgataaaa ttaaaaaagg
     5041 agatttaata gagaatattt tatctaaatt aagtattgaa gacaataaaa ttattttaga
     5101 taatcatgaa attaatttct ctggaacatt aaatggaggt aatggatttg tatctttaac
     5161 attctcaatc ttagaaggaa taaatgcagt tatagaagtt gatttattat ctaaatcata
     5221 taaagttctt atttctggtg aactaaaaac attgatggca aattcaaatt ctgttcaaca
     5281 gaaaatagat tatataggat tgaacagcga attacaaaaa aatatacctt atagttttat
     5341 ggatgataaa ggaaaagaaa atggatttat taattgttct acaaaagaag gtttatttgt
     5401 atctgaatta tctgatgtag ttcttataag taaagtttat atggacaata gtaaacctct
     5461 atttggatat tgtagtaatg atttgaaaga tgttaaagtc ataactaaag atgacgttat
     5521 tatattaaca ggatattatt taaaagatga tataaaaatc tctctttctt ttactataca
     5581 agatgaaaat actataaaat taaatggagt atatttagat gaaaatggag tagctgaaat
     5641 attgaaattt atgaataaaa aaggtagtac aaatacttca gattctttaa tgagcttttt
     5701 agaaagtatg aatataaaaa gtattttcat aaattcctta caatctaata ctaagcttat
     5761 attagatact aattttataa taagtggtac tacttctatt ggtcaatttg agtttatttg
     5821 tgataaagat aataatatac aaccatattt cattaagttt aatacactag aaactaaata
     5881 tactctatat gtaggtaata gacaaaatat gatagtagaa ccaaattatg atttagatga
     5941 ttctggagat atatcttcaa ctgtcattaa tttttctcag aaataccttt atggaataga
     6001 cagttgtgtt aataaagtta taatttcgcc aaatatatat acagatgaaa taaacataac
     6061 acctatatat gaagcaaata atacttatcc agaagtgatt gtattagata caaattatat
     6121 aagtgaaaaa atcaatatta atatcaatga tttatctata cgatatgtat ggagtaatga
     6181 tggaagtgat tttattctta tgtcaactga tgaagagaac aaggtatcac aagttaaaat
     6241 aagatttact aatgttttta aaggtaatac tatatcagat aagatatctt ttaattttag
     6301 tgataagcaa gatgtatcta taaataaagt tatttcaaca tttacacctt catattatgt
     6361 ggaaggatta cttaattatg atttaggtct gatttcttta tacaatgaga aattttatat
     6421 taataacttt ggaatgatgg tgtctggatt agtatatatt aatgattcat tatattattt
     6481 caagccacca ataaagaact tgataactgg atttacaact ataggtgatg ataaatacta
     6541 ctttaatcca gataatggtg gagctgcttc agtcggagaa acaataattg atggcaaaaa
     6601 ctactacttc agccaaaatg gagtgttaca aacaggtgta tttagtacag aagatggatt
     6661 taaatatttt gctccagcag atacacttga tgaaaatcta gagggggaag caattgattt
     6721 tactggcaaa ctaactattg atgaaaatgt ttattatttt ggagataatt atagagcagc
     6781 tatagaatgg caaacattag atgatgaagt gtactatttt agtacagata caggtagagc
     6841 ttttaaaggg ctaaatcaaa taggtgatga taaattctat ttcaactctg atggtattat
     6901 gcaaaaagga tttgttaata taaatgataa gacattctat tttgatgatt ctggtgtgat
     6961 gaagtcagga tatactgaaa tagatggaaa atatttttac tttgctgaga atggagaaat
     7021 gcaaatagga gtatttaata cagcagatgg atttaaatat tttgctcatc atgatgaaga
     7081 tttaggaaat gaagaaggtg aagcactttc atattctggt atacttaatt ttaacaataa
     7141 gatttattat tttgatgatt catttacagc agtagttgga tggaaggatt tagaagatgg
     7201 ttcaaaatat tactttgatg aagatacagc agaagcatat ataggtatct caataattaa
     7261 tgatggtaaa tattatttta atgattctgg aatcatgcaa attggatttg tcacaataaa
     7321 taatgaagta ttttatttct ctgattctgg aatagtagaa tctggaatgc aaaatataga
     7381 tgataattat ttctatatag acgaaaatgg tctagttcaa attggtgtat ttgacacttc
     7441 agatggatat aaatactttg caccagctaa tactgtaaat gataatatct atggacaagc
     7501 agttgaatat agtggtttag ttagagttgg tgaagatgta tattattttg gagaaacata
     7561 tacaattgag actggttgga tatatgatat ggaaaatgaa agtgataaat attatttcga
     7621 tccagaaact aaaaaagcat ataaaggtat taatgtaatt gatgatataa aatactattt
     7681 tgatgagaat ggaataatga gaacaggtct tataacattt gaagataatc attactattt
     7741 taatgaagat ggtattatgc aatatggtta tctaaatata gaagataaga cgttctactt
     7801 tagtgaagat ggtattatgc agattggagt atttaataca ccagatggat ttaaatattt
     7861 tgcacatcaa aatactttag atgagaattt tgagggagaa tcaataaact atactggttg
     7921 gttagattta gatgaaaaga gatattattt tacagatgaa tatattgcag caactggttc
     7981 agttattatt gatggtgagg agtattattt tgatcctgat acagctcaat tagtgattag
     8041 tgaatagata aaaatacgtt aaatatatcc tcttatactt aaatatataa aaataaacaa
     8101 aatgatacac tacataaagt gttctatcta atatgaagat ttaccaataa aaaggtgaac
     8161 tatgatgaat gcacagtagt tcaccttttt atatttctaa tggtaacaaa atattttttt
     8221 atataaacct aggaggcgtt atgaatatga caatatcttt tttatcagag catatattta
     8281 taaagttagt aattttaact atatcatttg atacattatt aggatgttta agtgcaataa
     8341 aaagtcgtaa atttaattct agttttggaa tagatggagg aatcagaaaa gtagcaatga
     8401 tagcatgtat atttttttta tcagtagttg acattcttac aaagtttaac tttttattta
     8461 tgttaccaca agattgtatc aattttttaa gactaaaaca tcttggaata tctgaatttt
     8521 tctctatttt atttatttta tatgaaagtg taagcatatt aaaaaatatg tgcttatgtg
     8581 gattaccagt acctaagaga ttaaaggaaa aaatagcagt tttactagat gcaatgacag
     8641 atgaaatgaa tgctaaggat gaaaagtaag taatggtaga tataataaaa atattaacaa
     8701 ataaaaagtg ttatccaaat aagaatagct gaaagttatc ataattcatg aaactgataa
     8761 tgaaaacgag ggagcagatg ccaagagaca cacaagtatt aaatacatat aatttcgaag
     8821 caagtgttca ttactatatg gatgacaagg tagtatatca aacattggtt cacaaagatg
     8881 gtgcatggtc agttggtaaa atctattaag ctacattagt tacagatatc acaaactata
     8941 atagttaaac atagaaatat gtgtaaattg tgatggaaat tattcaaaaa cacaaaaata
     9001 cgtgatgaag gacaaaatga tagagaaaat aagtatcaaa ccttaataaa tgatttaatt
     9061 gatagtttaa aagttatagg aaaaatatat aaagaaataa aaacattaaa aaaatataag
     9121 atatgtttac aaattactat cagacaatct ccttatctaa tagaagagtc aattaactaa
     9181 ttgagtatct ttaaattgaa atgttaggaa gtgatttaaa tatgaaaact taaattataa
     9241 aaaatcaata ttaatttatc tttaaaaaat agaaaggagt gtataagatt tattttcaaa
     9301 gtttaaaaac aagaaaatca atttaaattt cagaaggaat aaatgtggtt atagaagtgg
     9361 atttattatc aaaaataata gtactaggag gtttttatgt ctttaatatc taaagaagag
     9421 ttaataaaac tcgcatatag cattagacca agagaaaatg agtataaaac tatactaact
     9481 aatttagacg aatataataa gttaactaca aacaataatg aaaataaata tttacaatta
     9541 aaaaaactaa atgaatcaat tgatgttttt atgaataaat ataaaaattc aagcagaaat
     9601 agagcactct ctaatctaaa aaaagatata ttaaaagaag taattcttat taaaaattcc
     9661 aatacaagtc ctgtagaaaa aaatttacat tttgtatgga taggtggaga agtcagtgat
     9721 attgctcttg aatacataaa acaatgggct gatattaatg cagaatataa tattaaactg
     9781 tggtatgata gtgaagcatt cttagtcaat acactaaaaa aggctatagt tgaatcttct
     9841 accactgaag cattacagct actagaggaa gagattcaaa atcctcaatt tgataatatg
     9901 aaattttaca aaaaaaggat ggaatttata tatgatagac aaaaaaggtt tataaattat
     9961 tataaatctc aaatcaataa acctacagta cctacaatag atgatattat aaagtctcat
    10021 ctagtatctg aatataatag agatgaaact ttattagaat catatagaac aaattctttg
    10081 agaaaaataa atagtaatca tgggatagat atcagggcta atagtttgtt tacagaacaa
    10141 gagttattaa atatttatag tcaggagttg ttaaatcgtg ggaatttagc tgcagcatct
    10201 gacatagtaa gattattagc cctaaaaaat tttggcggag tatatttaga tgttgatatg
    10261 cttccaggta ttcactctga tttatttaaa acaataccta gacctagctc tattggacta
    10321 gaccgttggg aaatgataaa attagaggct attatgaagt ataaaaaata tataaataat
    10381 tatacatcag aaaactttga taaacttgat caacaattaa aagataattt taaactcatt
    10441 atagaaagta aaagtgaaaa atctgagata ttttctaaat tagaaaattt aaatgtatct
    10501 gatcttgaaa ttaaaatagc tttcgcttta ggcagtgtta taaatcaagc cttgatatca
    10561 aaacaaggtt catatcttac taacctagta atagaacaag taaaaaatag atatcaattt
    10621 ttaaaccaac accttaaccc agccatagag tctgacaata acttcacaga tactactaag
    10681 atttttcatg attcactatt taattcagct accgcagaaa actctatgtt tttaacaaaa
    10741 atagcaccat acttacaagt aggttttatg ccagaagctc gctccacaat aagtttaagt
    10801 ggtccaggag cttatgcatc agcttactat gatttcataa atttacaaga aaatactata
    10861 gaaaaaactt taaaagcatc agatttaata gaatttaaat tcccagaaaa taatctatct
    10921 caattgacag aacaagaaat aaatagtcta tggagctttg atcaagcaag tgcaaaatat
    10981 caatttgaga aatatgtaag agattatact ggtggatctc tttctgaaga caatggggta
    11041 gactttaata aaaatactgc cctcgacaaa aactatttat taaataataa aattccatca
    11101 aacaatgtag aagaagctgg aagtaaaaat tatgttcatt atatcataca gttacaagga
    11161 gatgatataa gttatgaagc aacatgcaat ttattttcta aaaatcctaa aaatagtatt
    11221 attatacaac gaaatatgaa tgaaagtgca aaaagttact ttttaagtga tgatggagaa
    11281 tctattttag aattaaataa atataggata cctgaaagat taaaaaataa ggaaaaagta
    11341 aaagtaacct ttattggaca tggtaaagat gaattcaaca caagcgaatt tgctagatta
    11401 agtgtagatt cactttccaa tgagataagt tcatttttag ataccataaa attagatata
    11461 tcacctaaaa atgtagaagt aaacttgctt ggatgtaata tgtttagtta tgattttaat
    11521 gttgaagaaa cttatcctgg taagttacta ttaagtatta tggacaaaat tacttccact
    11581 ttacctgatg taaataaaga ttctattact ataggagcaa atcaatatga agtaagaatt
    11641 aatagtgagg gaagaaaaga acttctagct cactcaggta aatggataaa taaagaggaa
    11701 gctattatga gcgatttatc tagtaaagaa tacatttttt ttgattccat agataataag
    11761 ctaaaagcaa agtccaagaa tattccaggt ttagcgtcaa tatcagaaga tataaaaaca
    11821 ttattacttg atgcaagtgt tagtcctgat acaaaattta ttttaaataa tcttaagctt
    11881 aatattgaat cttctattgg tgattacatt tattatgaaa aattagaacc tgttaaaaat
    11941 ataatccaca attctataga tgatttaata gatgagttca atctacttga aaatgtatct
    12001 gatgaattat atgaattaaa aaaattaaat aatctagatg agaagtattt aatatctttt
    12061 gaagatatct caaaaaataa ttcaacttat tctgtaagat ttattaacaa aagtaatggt
    12121 gaatcagttt atgtagagac agaaaaagaa attttttcaa aatatagcga acatattaca
    12181 aaagaaataa gtactataaa gaatagtata attacagatg ttaatggtaa tttattggat
    12241 aatatacagt tagatcatac ttctcaagtt aatacattaa acgcagcatt ctttattcaa
    12301 tcattaatag attatagtag caataaagat gtactgaatg atttaagtac ctcagttaag
    12361 gttcaacttt atgctcaact atttagtaca ggtttaaata ctatatatga ctctatccaa
    12421 ttagtaaatt taatatcaaa tgcagtaaat gatactataa atgtactacc tacaataaca
    12481 gaggggatac ctattgtatc tactatatta gacggaataa acttaggtgc agcaattaag
    12541 gaattactag acgaacatga cccattacta aaaaaagaac tagaagctaa ggtgggtgtt
    12601 ttagcaataa atatgtcatt atctatagct gcaacggtag cttcaattgt tggaataggt
    12661 gctgaagtta ctattttctt attacctata gctggtatat ctgcgggaat accttcatta
    12721 gttaataatg aattaatatt gcatgataag gcaacttcag tggtaaacta ttttaatcat
    12781 ttgtctgaat ctaaagaata tggccctctt aaaacagaag atgataaaat tttagttcct
    12841 attgatgatt tagtaatatc agaaatagat tttaataata attcgataaa actaggaaca
    12901 tgtaatatat tagcaatgga ggggggatca ggacacacag tgactggtaa tatagatcac
    12961 tttttctcat ctccatatat aagctctcat attccttcat tatcagttta ttctgcaata
    13021 ggtataaaaa cagaaaatct agatttttca aaaaaaataa tgatgttacc aaatgctcct
    13081 tcaagagtgt tttggtggga aactggagca gttccaggtt taagatcatt ggaaaataat
    13141 gggactaaat tgcttgattc aataagagat ttatacccag gcaaatttta ctggagattc
    13201 tatgcctttt tcgattatgc aataactaca ttaaaaccag tgtatgaaga cactaatact
    13261 aaaattaaac tagataaaga tactagaaac tttataatgc caactataac tactgacgaa
    13321 attagaaaca aattatctta ttcatttgat ggagcaggag gaacttactc tttattatta
    13381 tcttcatatc caatatcaat gaatataaat ttatctaaag atgatttatg gatatttaat
    13441 attgataatg aagtaagaga aatatctata gaaaatggta ctattaaaaa aggaaattta
    13501 atagaagatg ttttaagtaa aattgatata aataaaaata aacttattat aggcaatcaa
    13561 acaatagatt tttcaggtga tatagataac aaagatagat atatattctt gacttgtgag
    13621 ttagatgata aaattagttt aataatagaa ataaatcttg ttgcaaaatc ttatagtttg
    13681 ttattgtctg gggataaaaa ttatttgata tccaatttat ctaatactat tgagaaaatc
    13741 aatactttag gcctagatag taaaaatata gcttacaatt acactgatga atctaataat
    13801 aaatattttg gagctatatc taaaacaagt caaaaaagca taatacatta taaaaaagac
    13861 agtaaaaata tattagaatt ttataatggc agtacattag aatttaacag taaagacttt
    13921 attgctgaag atataaatgt atttatgaaa gatgatatta atactataac aggaaaatac
    13981 tatgttgata ataatactga taaaagtata gatttctcta tttctttagt tagtaaaaat
    14041 caagtaaaag taaatggatt atatttaaat gaatccgtat actcatctta ccttgatttt
    14101 gtgaaaaatt cagatggaca ccataatact tctaatttta tgaatttatt tttgaacaat
    14161 ataagtttct ggaaattgtt tgggtttgaa aatataaatt ttgtaatcga taaatacttt
    14221 acccttgttg gtaaaactaa tcttggatat gtagaattta tttgtgacaa taataaaaat
    14281 atagatatat attttggtga atggaaaaca tcgtcatcta aaagcactat atttagcgga
    14341 aatggtagaa atgttgtagt agagcctata tataatcctg atacgggtga agatatatct
    14401 acttcactag atttttccta tgaacctctc tatggaatag atagatatat caataaagta
    14461 ttgatagcac ctgatttata tacaagttta ataaatatta ataccaatta ttattcaaat
    14521 gagtactacc ctgagattat agttcttaac ccaaatacat tccacaaaaa agtaaatata
    14581 aatttagata gttcttcttt tgagtataaa tggtctacag aaggaagtga ctttatttta
    14641 gttagatact tagaagaaag taataaaaaa atattacaaa aaataagaat caaaggtatc
    14701 ttatctaata ctcaatcatt taataaaatg agtatagatt ttaaagatat taaaaaacta
    14761 tcattaggat atataatgag taattttaaa tcatttaatt ctgaaaatga attagataga
    14821 gatcatttag gatttaaaat aatagataat aaaacttatt actatgatga agatagtaaa
    14881 ttagttaaag gattaatcaa tataaataat tcattattct attttgatcc tatagaatct
    14941 aacttagtaa ctggatggca aactatcaat ggtaaaaaat attattttga tataaatact
    15001 ggagcagctt caactagtta taaaattatt aatggtaaac acttttattt taataataat
    15061 ggtgtgatgc agttaggagt atttaaagga cctgatggat ttgagtattt tgcacctgcc
    15121 aatactcaga ataataacat agaaggtcag gctatagttt atcaaagtaa attcttaact
    15181 ttgaatggca aaaaatatta ttttgataat gactcaaaag cagtcactgg atggaggatt
    15241 attaacaatg agaaatatta ctttaatcct aataatgcta ttgctgcagt cggattgcaa
    15301 gtaattgaca ataataagta ttatttcaat cctgacactg ctatcatctc aaaaggttgg
    15361 cagactgtta atggtagtag atactacttt gatactgata ccgctattgc ctttaatggt
    15421 tataaaacta ttgatggtaa acacttttat tttgatagtg attgtgtagt gaaaataggt
    15481 gtgtttagtg gctctaatgg atttgaatat ttcgcacctg ctaatactta taataataac
    15541 atagaaggtc aggctatagt ttatcaaagt aaattcttaa ctttgaatgg taaaaaatat
    15601 tactttgata ataactcaaa agcagttacc ggatggcaaa ctattgatag taaaaaatat
    15661 tactttaata ctaacactgc tgaagcagct actggatggc aaactattga tggtaaaaag
    15721 tattacttta atactaacac tgctgaagca gctactggat ggcaaactat tgatggtaaa
    15781 aaatattact ttaatactaa cacttctata gcttcaactg gttatacaat tattaatggt
    15841 aaatattttt attttaatac tgatggtatt atgcagatag gagtgtttaa agtacctaat
    15901 ggatttgaat actttgcacc tgctaatact cataataata acatagaagg tcaagctata
    15961 ctttaccaaa ataaattctt aactttgaat ggtaaaaaat attactttgg tagtgactca
    16021 aaagcaatta ctggatggca aaccattgat ggtaaaaaat attactttaa tcctaataat
    16081 gctattgctg cgactcatct atgcactata aataacgaca agtattactt tagttatgat
    16141 ggaattcttc aaaatggata tattactatt gaaagaaata atttctattt tgatgctaat
    16201 aatgaatcta aaatggtaac aggagtattt aaaggaccta atggatttga gtattttgca
    16261 cctgctaata ctcataataa taacatagaa ggtcaggcta tagtttacca gaataaattc
    16321 ttaactttga atggcaaaaa atattatttt gataatgact caaaagcagt tactggatgg
    16381 caaactattg atagtaaaaa atattacttt aatcttaaca ctgctgttgc agttactgga
    16441 tggcaaacta ttgatggtga aaaatattac tttaatctta acactgctga agcagctact
    16501 ggatggcaaa ctattgatgg taaaagatac tactttaata ctaacactta tatagcttca
    16561 actggttata cgattattaa tggtaaacat ttttatttta atactgatgg tattatgcag
    16621 ataggagtgt ttaaaggacc tgatggattt gaatactttg cacctgctaa tactcataat
    16681 aataacatag aaggtcaagc tatactttac caaaataaat tcttaacttt gaatggtaaa
    16741 aaatattact ttggtagtga ctcaaaagca gttaccggat tgcgaactat tgatggtaaa
    16801 aaatattact ttaatactaa cactgctgtt gcagttactg gatggcaaac tattaatggt
    16861 aaaaaatact actttaatac taacacttat atagcttcaa ctggttatac aattattagt
    16921 ggtaaacatt tttattttaa tactgatggt attatgcaga taggagtgtt taaaggacct
    16981 gatggatttg aatactttgc acctgctaat acggatgcta acaacataga aggtcaagct
    17041 atacgttatc aaaatagatt cctatattta catgacaata tatattactt tggcaatgat
    17101 tcaaaagcgg ctactggttg ggcaactatt gatggtaata gatattactt cgagcctaat
    17161 acagctatgg gtgcgaatgg ttataaaact attgataata aaaattttta ctttagaaat
    17221 ggtttacctc agataggagt gtttaaagga cctaatggat ttgaatactt tgcacctgct
    17281 aatacggatg ctaacaatat agatggtcaa gctatacgtt atcaaaatag attcctacat
    17341 ttacttggaa aaatatatta ctttggtaat aactcaaaag cagttactgg atggcaaact
    17401 attaatagta aagtatatta ctttatgcct gatactgcta tggctgcagc tggtggactt
    17461 ttcgagattg atggtgttat atatttcttt ggtgttgatg gagtaaaagc ccctgggata
    17521 tatggctaaa atatatgttt gacaaaaaat tattcctgtg atactcagaa attattttta
    17581 tataataaat attgagattt aattaaaagt catgtgttat tgtaatacat gacttttagt
    17641 taaaattttt ctatcattta ataatctatt attcttgact attttataat aaaattcata
    17701 tatggaaata ttaatactaa ataattaata gttgatagaa aatagataat atgctaaaag
    17761 caaaaactaa tttagagcct tgtaactgtt tatttgcaat tataaaaaca tctttaaaca
    17821 tattgactat aatataaaat attaactata atacaaaaca atattaatta attttctcta
    17881 cagctatccc tggtatggtt atttttccac ccatagttga ttcataagtt atagttccag
    17941 cacttatacc tcttatagtt atgtaatcat cctctaacac tcttgaagga gttattgatt
    18001 ttttataact acatagtacg accttatcat aatttccaga cacagctaat cttatttgca
    18061 cctcatcacc atcttcaata acttgaataa ccttaccttc aaattttacc tttttgtact
    18121 tataatcatc aggtgttcta gctaattggt cataagtaat accagtatca tatcctttct
    18181 tctcttcttc ttctttttta cgttgttctt cagccttttt agcttcttca gctttacgtt
    18241 gattttcagc ttcaatagct ttcttttcgt cgtctttcat tttgaaccat ggttcagcat
    18301 cagacaattt gctatttaaa gtttctattt ctgttttttg agaatctata actgatgttt
    18361 gattagaaat gacctcctca tggtcttcag aacaagctgg tgaggatata ttgccaaaaa
    18421 acacaccaaa ataaatgcca gtagagcaat atcctttgtg ctcttaaaga atttaattat
    18481 tttcttagag cttccttttc tttcattact aaattcgtta ccctcatttt ttttagaaaa
    18541 cataatacaa tctccctttt attagatata ttttatttta aaatttaaaa acttaaaata
    18601 actagttgac taaaaattat atattcatat tttcctaaaa tatatttccc ctaagcataa
    18661 ttttacaaaa taaatcaaga agattcaata tattttgaaa aatgaggtaa aaatgtcaga
    18721 ttataatata attttcttga aggtgaattt tatatattaa atgaaacata aagtatttta
    18781 tttgtataaa actatattat gatgtaatta aatgattcag tactttagag gtggtaaaat
    18841 atagatagat gaaagagcat aaataaagtg atataataaa aaattaaata ttaactataa
    18901 taaaaaaata tctggtacta agtataatac aaaaataata tatcagaaag aaggtgaagt
    18961 atgggagaac tacaaaaaat tccaggggta gggaaagcaa cagaaaaatc tttaatcatg
    19021 ttaggatata caactattaa atcacttaaa gatgcaaacc cagcacaaat gtatgaaaag
    19081 gaatgtttaa tgcgtggaca gcacatagac agatgtcagt tatatgtata cagatgtgca
    19141 gtatattttg catcaactga aaatccagaa ccagaaaaat taaagtggtg gtattggaaa
    19201 gattaaaaaa tcaaaaaagt tagatataaa aaagagttga atatccgttt aaataataaa
    19261 aactaatact caactctttt ttagtcgtaa attttatgta aatctatttg tatggctaaa
    19321 tatacccatc aataaatgta taaatatacc aatcaataaa tgactaaaca tactacctaa
    19381 caaaggatta aataaattac ttaacaaagc attctaaaac aattaattag aaactgtttc
    19441 ttcaagccat tttctacatt gcccaatatt taccataggg tcaaagcctt ggtcttttcc
    19501 cttatttaaa agcatatcac aagggaaagt atcacattca ccacaatgat taagttcctt
    19561 actttcacag caagttttaa ccttacattc tccaccccaa aatggttttt ccatagttgg
    19621 gcaaccagtg caatttactt gttctttttt tgtacaactg tcacaacaga caccacatct
    19681 tgattcaaac atgttcaaac atcctttcca ttatttaaaa atattataaa atgattttag
    19741 atgactacaa aagtgcaaat agttgatttt aagtcatcta aatgttaaac ataaaattat
    19801 taatactggt aaatttataa aaactattcc aaaaatattt ttgaatattt caaaacacaa
    19861 taaattactt caaatccctt tgaatattcc ataacactat agattacttt aaatcccctt
    19921 gaatatattt cacagcatta tagattgctt taaatctctt tgaatatttc ataacactat
    19981 agattacttt aaatctcttt gaatattcta caagactata gattagttta aattcctttg
    20041 aatatttcat aacatcataa attagtttaa atctctttga atattctaca agactataga
    20101 ttagtttaaa tccctttgaa tatttcataa cactatagat tactttaaat ttctttgaat
    20161 attctacaag actatagatt agtttaaatc cctttgaata tttcataaca ccatagatta
    20221 ctttaaatcc ctttgaatat tctataacac tataaattac ttttaacttt tatataacca
    20281 taaagttttt atctggcact ttaaatggag tttttatatc aaatttgcct tccatcgaat
    20341 aaccaaactt aatttaatta cacattccaa gagtcaaata ttcattcaat atatttgata
    20401 attctatgtt atagccataa ttaaatatag attattaaag ttaaatattt atttaatgta
    20461 ttgaatgatt ttttagctat atttaattaa gtttcattat ataatataaa aattgttttt
    20521 gcattaagtt ttaatataac
//
LOCUS       DAMJWP010000049        20311 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.050, whole genome shotgun sequence.
ACCESSION   DAMJWP010000049 DAMJWP010000000
VERSION     DAMJWP010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20311
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.050"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(91..969)
                     /locus_tag="PC700_001522"
     CDS             complement(91..969)
                     /locus_tag="PC700_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF2580836.1"
                     /translation="MRYDIAIIGSGPAGLSAAINAKIRNKTIIMFGNDNLSNKLVKAP
                     SIDNYLGFYDISGDELKDKFKSHIDSMDISIENKRINNIYAMGEYFTIMSGNDMYEAT
                     TVILATGVEYTRPIKGEEEFLGRGVGYCATCDAPLYRNKKVAVIGYNEESKEEANFLS
                     ELTSKTYFIPMYKKDNLMRSSDNLDDSIEVIHDRPVQIDGDKLVNKVSFKENHIEVDG
                     VFVIKDSTAPSALVPGIEIDGIHIKVDNNMKTSIDGCFAAGDCVGKPYSYIKAAGQGQ
                     IAALNAVYYLDKLKRA"
     gene            complement(986..1303)
                     /gene="trxA"
                     /locus_tag="PC700_001523"
     CDS             complement(986..1303)
                     /gene="trxA"
                     /locus_tag="PC700_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDF2580837.1"
                     /translation="MAKVINTSEFRSSVEGSKGVVVVDFFATWCGPCNMLGPVFAELG
                     EEMKDKARFVKVDIDESLEIAQQFNVSTVPTMIIFKDGKPVETLIGFMPKNKIEMQVK
                     SYL"
     gene            complement(1453..2685)
                     /locus_tag="PC700_001524"
     CDS             complement(1453..2685)
                     /locus_tag="PC700_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2580838.1"
                     /translation="MINKNVFTSTKNHLIKMYIIVVGSFLIIFSIFIYSYFRGLTYSG
                     IDSEINDELEYIVSQFKRTSFLNPIRLKDPKDMVYVYEDGRISYYTQNEYFDELLPDR
                     RLDKKNSFFKYTENGYTFRELNVDVGRYQIQIIRNIDSEMNSLRQLTSVLIIGILISV
                     IITYFVAVYLTRKALIPIETAWKNQAKFIQDASHELRTPITIVSSKLESMLKSPESTV
                     NDEVETIATAMKETRRLKKMITDLLSLTKEDSIVKVNLEEIDLEKLLEEISEDYIDIA
                     EFQEKRFVFNSKLKNKVIITDKNKLRQLILIFIDNAFKYTKLGDEIALELKEDIEDEV
                     TLLISDTGIGIKKEEIPLIFDRFFRSENVRNKDLEGSGIGLSIARMISLNLSIDINVT
                     SDVDIGTTFELSIPKKLK"
     gene            complement(2675..3346)
                     /locus_tag="PC700_001525"
     CDS             complement(2675..3346)
                     /locus_tag="PC700_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2580839.1"
                     /translation="MKVLIVEDNKILLESVVEELSKHFETEKCEDGEEALYLVNQNIY
                     DLVILDLMLPNINGFDILKKMRVNNIDTPVLILTAKETLDDKVEAFTIGANDYLTKPF
                     YMEELVARVYAILRTNGKIKERNGLEFKSLYLDTLEKRVYIEKEEIKLQNKQFNLLEY
                     FVLNKGSILLKEQIYDRIWGIDSDATIEIVEVYVSNLRKKLSKYGYDKYIKTKRKVGY
                     IFDDK"
     gene            complement(3362..4198)
                     /locus_tag="PC700_001526"
     CDS             complement(3362..4198)
                     /locus_tag="PC700_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580840.1"
                     /translation="MNKIVKKSISLMIILTIFIFMLTACEKDEQPDSVDIQTEDKNEI
                     KIDEGNAKVLNIGKSEIINIDEGDKVDTSTKVENNSTFNISNVELIYNEYDENKKIIS
                     SDSKALLDMTLMPGKVAYIECGHKTFVKGVEVYAYEYEAEGKIVYVNLKENTINIRNN
                     NIKLENSSQYEVLSTSELKKVNESNEGITYQVKVKNSSSKDLGNIILKTAEVNENGEY
                     LTVNRVPSYKILKPSEETDIDILCSTKAKSVEIVGYTYDDIKEKANVDIDLKSHKVKI
                     DK"
     gene            complement(4224..4898)
                     /locus_tag="PC700_001527"
     CDS             complement(4224..4898)
                     /locus_tag="PC700_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF2580841.1"
                     /translation="MNNNEDRIDKFLELQSKGFKFAEIAQNLKISQSTLRTFLNKKGY
                     KSEKGKYILKDELNYNKSLDNRNLEEEKAKEYEKSKKNTISNRKKVSTKKKISNEKAL
                     SKSVEQIEFNDTGLKNAKRKQNVKPKKDRKINITQEDLDKLCEVYDWYLEVKDYNIKP
                     KKVSNKKDVHIDDEHIDELKTTRIRIDKEIWNDFERLCSNSNFEKHQILTQALKEFMK
                     KYKNLL"
     gene            complement(4912..5640)
                     /locus_tag="PC700_001528"
     CDS             complement(4912..5640)
                     /locus_tag="PC700_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDF2580842.1"
                     /translation="MMFDRGKWNKLDYNELLEYMMSIKDIKYRDFNKKLIPGTENIIG
                     IRVPNLRKLSKEISQGNWKEFLEVAEDTYYEEVRLQGMVIGNINSNFEETLYYVKRFI
                     PKIDNWSVCDGFCSGLKSMKKYKENMYDILKKYVYSKNPWEIRFALVMFLIYYVDDKH
                     IKEIFEYCNNIQSEEYYVKMGMAWLLSICFIKCEEETFLYIKNNNLDDFTYNKMLQKI
                     IESNRVDLEKKNIIRSMKRKTRRC"
     gene            complement(5767..6456)
                     /locus_tag="PC700_001529"
     CDS             complement(5767..6456)
                     /locus_tag="PC700_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2580843.1"
                     /translation="MDRYSEITNKNQREIVLLKGFPCIWGKCSFCDYIDDNSNLEEEM
                     NKLNLRVLKNVTGKYGVLEVINSGSCFELPKDTLEKIKCIIKEKNIKKLFLESHWSYK
                     NRLKEMREYFEIPVVFKIGVETFDNDFRNNILNKNANFKTPQDVKEYFDSPCIMIGIK
                     GQTKEMIDKDIEIILNTFEKATVNVFINNSSSIKRDEELVKWFVSKYKFLDENPNIEV
                     LYNNTDFGVGD"
     gene            complement(6464..7027)
                     /locus_tag="PC700_001530"
     CDS             complement(6464..7027)
                     /locus_tag="PC700_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF2580844.1"
                     /translation="MKRKICVKTITLIAFGISINIIGAFIAMGLRLPVYLDSIGTIMI
                     ASLLGPKYAVVTGVFGSLISGITFDVYSLYFAPVQISTGLLAGIAFKKNFLRGLKTPL
                     GVLLFAIPTSIISSIISAFLFGGMTSSGSSYIVQILKVLGLGDVFSVFVTQVFTDYGD
                     KLLAVVLVNLGLNAVPKTLKVSLTGGK"
     gene            complement(7029..7997)
                     /locus_tag="PC700_001531"
     CDS             complement(7029..7997)
                     /locus_tag="PC700_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HDF2580845.1"
                     /translation="MEKRKVIIDCDPGIDDSLAILLALNSPELEVIGITTCCGNVPAN
                     IGAENALKTLQMCSSLNIPVYIGEEAPLKRKLVTAQDTHGEDGIGENFYQKVVGAKAK
                     NGAVDFIINTLHNHEKVSIIALAPLTNIAKALIKDKKAFENLDEFVSMGGAFRIHGNC
                     SPVAEFNYWVDPHGADYVYKNLSKKIHMVGLDVTRKIVLTPNIIEFINRLDKKMAKYI
                     TEITRFYIDFHWEQEGIIGCVINDPLAVAYFIDRSICKGFESYVEVVEDGIAMGQSIV
                     DSFNFYKKNPNAIVLNEVDEKKFMYMFLKRLFKGYEDIIDSVEGVI"
     regulatory      complement(8098..8141)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            8506..9135
                     /locus_tag="PC700_001532"
     CDS             8506..9135
                     /locus_tag="PC700_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2580846.1"
                     /translation="MSIKERRKNEKEEMKKKIMDASIEIINQHGYENLSIRKIATKIE
                     YSPTTIYLYYKDKAEIISDMTNKLYNTVESNAIDIMNNCSSLPIDKQIIEIMTSFIKT
                     LSSEPEMAKAIMHSRMNIIFASENTNNTPSNNGIKMLDKFLSIGIEQGIFKKNIDKSS
                     WMLISALLGFVLCVVENKLYSLHNFSQLIENFLDILVGGLYNENSQQSY"
     gene            9110..9514
                     /locus_tag="PC700_001533"
     CDS             9110..9514
                     /locus_tag="PC700_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HDF2580847.1"
                     /translation="MKIRNKATNKKKKYVKISIALFFVFICIIIVIVSNLKPENLVVK
                     DIDINNVKNGIYTGSADNNLVKATVSVEVNNGKIQNINILKHDHLLGKPAEKITTSII
                     KQQSLDVDAITSATYSSNTIRKAVENALRKGE"
     gene            9517..9990
                     /locus_tag="PC700_001534"
     CDS             9517..9990
                     /locus_tag="PC700_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDF2580848.1"
                     /translation="MNTIIIYSSKYGCTKDCANILKNKLSDNVTIVDINNNNKIELSK
                     FDKIIIGSSIYVGSVSKKIQVLCNDNVELLNKKQVGIFLCCGFSEQADKYLKSNFPSS
                     LLESANAIGIFGSEARLEKMKFLDKLIMKAVSKGNYDSFRISQDNIDNFLINLNS"
     gene            10537..11202
                     /locus_tag="PC700_001535"
     CDS             10537..11202
                     /locus_tag="PC700_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HDF2580849.1"
                     /translation="MIKLIGILIVVIGFILKIDTLFTVLLAGVATGIVAGLDFNQIFT
                     ILGDSFVSNRGVSLFILTLPVIGVLERYGLKQRAVSLIEKLKRLTTGKVLTIYMIARQ
                     IAGALSIRMSGHPQFVRPLVNPMAQAAGLSNSDELKESDEEAIKALSAASENYGNFYG
                     QNLFAGSSGVLLIASTLTQFGYNVTGLNIVKASTIMAVIALVVATIQFTLYDKKLNKS
                     HKK"
     gene            11224..12156
                     /locus_tag="PC700_001536"
     CDS             11224..12156
                     /locus_tag="PC700_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HDF2580850.1"
                     /translation="MQNFINISLEIFYALMGFLMIVIAYKSFTTINNNKKYGTSLFWI
                     LISLPFIFGRLIPANIIGIILILSSLLTLSKQVVFAKYEEPDENFGKEQADKLKNKIF
                     IPSLILAFAAVVVAMSLSNFDNSSQFAIGVGSIIALISALIITKAKPATSVQDGSRLL
                     QQMGPASMLPQLLVALGALFTQAGVGEVISTMISGVVPADSRLFGVIAYVLGMVIFTM
                     IMGNAFAAFSVITAGIGIPFVLSQGGNPAIIGALALTAGYCGTLLTPMAANFNIVPAA
                     LLECKNDYIVIKYQAPVALVLIIAHILVMYFLGF"
     gene            12177..12818
                     /gene="pcp"
                     /locus_tag="PC700_001537"
     CDS             12177..12818
                     /gene="pcp"
                     /locus_tag="PC700_001537"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HDF2580851.1"
                     /translation="MKILLTGFDPFGGELINPAQEAVERVNNNINGAEIIKITIPTVM
                     TKSVEAIDKAIQEHNPDIVISVGQAGGRFDITPERVAINIDDFRIKDNEGNQVIDTII
                     KEDGEPAYFSKLPVKAMVKHMNENKIPASVSNTAGTFVCNHVMYGILYMIDKKYPNIK
                     GGFIHIPYTTSQVINKKNTPFMSLEEIVKGLELAIEACIIYKEDVKEIGGEIS"
     gene            complement(12937..13269)
                     /locus_tag="PC700_001538"
     CDS             complement(12937..13269)
                     /locus_tag="PC700_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2580852.1"
                     /translation="MSIDCISNTDLSETGFSYTLSLISGKYKMIILYCLVEFEVVRYN
                     ALKRYINTISYKTLSLSLKELEADNLIIRTEYPQIPPKVEYSLSERGKSLIPILDAMC
                     EWGERNRP"
     gene            13535..14155
                     /locus_tag="PC700_001539"
     CDS             13535..14155
                     /locus_tag="PC700_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2580853.1"
                     /translation="MKVLLINGSPNQYGCTYTALNEITKVLSKHDIKTEILYLGKEAI
                     PGCISCASCFETGKCIRNDKVNELIEDLDNIDGIIIGSPVYFSSATGQLTSFLDRLFF
                     IAGSRMATKLGASVVSCRRGGASATFDQLNKYFSISNMPIVSSQYWNQVHGFTPEDVM
                     KDEEGLQTMRTLGENMAWLLKCISAGKKAGIKEPQYEETIMTNFIK"
     gene            complement(14480..15304)
                     /locus_tag="PC700_001540"
     CDS             complement(14480..15304)
                     /locus_tag="PC700_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HDF2580854.1"
                     /translation="MDFFKKGISGFTIKILALIFMTFDHIAAFMPQTMQIPIWFHWVG
                     RISAPLFIFMAVEGFYHTSNRKKYISRLYIWSVIMAIGNQIINNVFSHPEGAIIINNI
                     FSTLFLIAIYLQAIEFIKKFRKEKEIKYFIIGLLMIIIPIILGIFTVALLFKVTNRVI
                     TLFMILVPVPFLVEGGPIWIILGIIFYLCRGKKFSLSICYVLMCIFIFTTMSNGDYSL
                     KNSILQNYQWMMIASLPLMLLYNGEKGKSMKYLFYLYYPIHVYILYILGIYLINGF"
     gene            complement(15315..16718)
                     /locus_tag="PC700_001541"
     CDS             complement(15315..16718)
                     /locus_tag="PC700_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2580855.1"
                     /translation="MKWKITRNFIFTIVFVAISVVIINIISILYVISTNSFFKVVDSG
                     NNPEEFARSFEKDLYEKDGEFKLSKIGAEKLEKSNSWIQVLNDLGEEVYGVNVPKDTP
                     KKYTPFQMVNNYKYIETKYVNFVLEKYLNKKHLNIIVGIPSRDISRIILTYSQNNIKK
                     TLNKVIIITLVIDSVVALGVGYLFSRKLTKPISSVLWSIETMANGNYSLYLKDRGIYE
                     EVFKNINMLADTLRVNEVERKENEELREEWLANITHDIKTPLASIQGYAEIINDKDYE
                     FEEDEIREYTEIIYNKSKYIKDLVDDLNLSTRLKNNTIVLDKKKINLVSLVRDIIIDI
                     LNDNRYKNRNIEFESNEDLIEVYVDSILFRRAITNLIFNSIVHNSEGTLISVEIVKKD
                     NIEIIIKDNGIGISKSDLKHIFKKYYRGTNTGEMHKGSGLGMAISKEIIEIHKGKIYV
                     SSEIGIGTKIIIEIKQN"
     gene            complement(16722..17447)
                     /locus_tag="PC700_001542"
     CDS             complement(16722..17447)
                     /locus_tag="PC700_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2580856.1"
                     /translation="MENRVLIIDDEVEILKLLETVLKKEGLNNIYTAKTKKEGLELFK
                     SINPDLIVLDIMLPDGEGYDICKEIRKTSNSPIIFLSAKTEELDKLLGLAIGGDDYVT
                     KPFSPKEVAFRVKAHLRRLSYFSDVQNESKNLNKNEEKIISFGPYILNESRAELIKDG
                     KSIGLSAKELKILSLFAHNQNQIISKEKLWDKVWGEDYVGFDNTIMVHIRKIREKLED
                     NPSKPEYILTIKGLGYKLAVKED"
     gene            complement(17559..18332)
                     /locus_tag="PC700_001543"
     CDS             complement(17559..18332)
                     /locus_tag="PC700_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2580857.1"
                     /translation="MMYLVKAEMIKILKSKSVWIIWLFLIFLGFLLIRKFDVLDTYAD
                     IFYKIEGSIPLIGLIMFIITSGNYIKEYDSNMTGLINTTKKGKKSVVLSKLVANGLIL
                     SIINISFILLVGIRGFSFFDFKNLNEPIHNLWYFGNSNLNLTVIQMYIIVILTTIFAS
                     FIFAQIGLTLSSIFKSAVIPFILGGLIMAIPYFSVGFIPDKAIKFMSVTPNWIMMSQQ
                     MVRYNVPSILIVFSIVISIILMIVLTKITYENFTSSKRF"
     gene            complement(18338..19225)
                     /locus_tag="PC700_001544"
     CDS             complement(18338..19225)
                     /locus_tag="PC700_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2580858.1"
                     /translation="MNKLEIENLSKIYGKKVANDKITVTLENGVYGLLGPNGAGKSTL
                     MKQITTLIKPDEGQILYNGEDIFKMDANYRNILGYLPQEFGVYKNFTAKEFLQYIGAL
                     KGLRGKYLNSKIGELLDLVGLYDVRNKSIGKFSGGMKRRVGIAQVLLNDPKIIVFDEP
                     TAGIDPQERARFRNLIAKMSRDKIIILSTHIISDIESVAKETIMIKDGKLLMKGTHKD
                     ILSHMENKVYNVQVKNECEVDDIQSKYKVVSIQSDIDSVVLRIVSEDIPSGDNIQPVQ
                     AKFEDVYMFYFDLEDAREV"
     gene            complement(19239..20261)
                     /locus_tag="PC700_001545"
     CDS             complement(19239..20261)
                     /locus_tag="PC700_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580859.1"
                     /translation="MVNWEIKKLAKSKSMFISLGILVLILMISIFITPVLETERYYID
                     KEKGCIEDTRSGIDIGNEKFQNKINVLKQFSNQTDTGEFSKKIELMSKKKLDNLSVNE
                     YKDISFWKVFNYRVTNSLINVGMLIIISIIISNIYVDEVVTSVKDIILSSKEKKKALK
                     SKIFVALLVPLVIYSVYLMGIFIITCMQQGVPVNGDLESFRIVDNVVALKTNLSITKY
                     VILNIGIELLMFEGWAMIAIFGSFISKSSISSISFFIFIISITKIMSVIHILPKKLLS
                     VVSNVNYYDLIFGFNKIIGNYLGDIMIFKVNIDIVNIAIGLLMAILIGAMSLCFLVIK
                     SDYISR"
BASE COUNT         7039 a         3247 c         2165 g         7860 t
ORIGIN      
        1 aacttaaaaa taaaaaactg tcttgaaata ataaaattat ttcaagacag ttttttataa
       61 atatctttaa aaagttaatc aatataaata ttaagccctc tttaatttat ctaaatagta
      121 aacagcattt aaagctgcta tttgcccttg accagctgct tttatataag aatagggttt
      181 tccaacacaa tctccagcag caaagcatcc atctatacta gtcttcatat tattgtcaac
      241 tttaatatga atgccatcaa tctctattcc aggaaccaat gcacttggag ccgtactatc
      301 ttttataacg aatactccat caacttcaat gtggttttct ttaaaagata ctttgtttac
      361 aagtttatct ccatctatct gtactggtct gtcatgtata acttcaatag aatcatctag
      421 gttatctgaa cttctcataa gattatcttt tttgtacata ggtataaaat aagtctttga
      481 agtgagttca cttaaaaaat ttgcttcttc tttagattct tcattatatc ctataactgc
      541 taccttttta tttctataaa gtggtgcatc acatgtagca cagtagccta cacctcttcc
      601 tagaaattct tcttcaccct tgattggtct agtatactct accccagttg caaggattac
      661 agtagtagct tcatacatat catttccaga cattattgta aagtattctc ccatagcata
      721 aatattattt attcttttat tttcaattga aatatccata ctatctatat gtgatttaaa
      781 cttgtctttt aattcatcac cacttatatc ataaaaacct aaataattat ctattgaagg
      841 agctttaaca agcttattac ttaaattatc attaccaaac atgataattg ttttatttct
      901 tatttttgca tttatagcag cagacaatcc agctggacca ctgccgatta tagctatatc
      961 atatctcata atactcaccc attttttata aatatgattt tacttgcatt tcaattttgt
     1021 tctttggcat gaatccaatt aaagtttcta ctggttttcc atctttaaat ataatcatag
     1081 ttggtactgt agatacattg aattgttgag ctatttctaa gctctcatct atatcaactt
     1141 ttacaaatct agctttatcc ttcatctctt cacctaattc tgcaaataca ggtcccaaca
     1201 tgttacaagg tccacaccaa gttgcaaaga aatcaactac aactactccc ttactacctt
     1261 caacactact tctaaattca gatgtattta taacttttgc catttttaaa tcctcctatt
     1321 aaactttatt atccctatat aattatacta tgaaatttat aaatataaat ttacttttat
     1381 aaggtctttt tatattttat actaattagt atacccattc ttcatttcta aaaacaattt
     1441 tttatatttt tattatttta acttttttgg tattgacaat tcaaatgtag ttccaatatc
     1501 tacatcagaa gttacattta tatcaataga taagtttaaa ctaatcatgc gagctattga
     1561 aagtcctatt ccagaacctt ctaaatcttt atttcttaca ttttcacttc taaaaaatct
     1621 atcaaatata agaggtatct cttctttttt tatgcctata ccagtgtcac ttataagtaa
     1681 agttacctca tcctctatat cttcttttaa ttctaatgct atctcatcac ccaattttgt
     1741 atatttaaag gcattatcaa tgaaaattaa tataagctgt cttaatttat ttttatctgt
     1801 tattataact ttattcttca gtttagaatt aaacacgaat cttttttcct gaaactcagc
     1861 aatgtcaata tagtcttctg atatttcttc caataatttt tctaagtcta tttcttctaa
     1921 attaacttta acaatagagt cctctttagt aagagacaat aaatcagtta tcattttttt
     1981 aagtcttcta gtttccttca ttgcagtagc tattgtttca acttcatcat ttactgtact
     2041 ttctggggat tttaacatac tttctaattt tgatgaaact attgttattg gtgttcttaa
     2101 ttcatgagaa gcatcttgta taaacttagc ttgatttttc catgctgttt ctattggaat
     2161 taaagctttt ctagtgagat atacagccac aaagtaagta attattacag aaattaatat
     2221 tccaattata agaacagatg taagctgtct taaagaattc atctcagaat ctatatttct
     2281 aattatctgt atttggtatc tacctacatc tacatttaat tctctaaaag tatatccatt
     2341 ttctgtgtat ttaaaaaacg aatttttttt atctagtctt ctatctggaa gtaactcatc
     2401 aaaatattca ttttgagtat agtaacttat ccttccatct tcatatacat acaccatatc
     2461 tttaggatct ttcagtctta ttgggttcaa aaatgaagtt cttttaaatt gagagactat
     2521 atattcaagt tcatcattta tctcactatc aattccacta tatgtaagtc ccctaaaata
     2581 tgagtatata aatatagaaa agattattaa aaaactacct actacaataa tatacatttt
     2641 tattaaatga tttttagtac tggtaaatac attcttattt atcatcgaat atatatccta
     2701 ccttacgttt agttttaata tatttatcat atccatattt acttagcttt tttcttagat
     2761 tacttacata tacttccact atttcaatag ttgcatctga atcaattccc catattctat
     2821 cataaatctg ctcttttaat aaaattgaac ctttatttaa tacaaaatac tcaagcaagt
     2881 taaactgttt gttttgaagt tttatttctt ctttttcaat atacactctt ttttctagtg
     2941 tatctaaata taaagattta aattctaatc cgtttctttc ttttatcttt ccattagtcc
     3001 ttaatatagc gtatacccta gctactaact cttccatata aaaaggtttt gtcaaataat
     3061 cattagctcc tatagtaaat gcttctactt tgtcatctaa tgtttctttt gctgtcaata
     3121 taagtacagg tgtatctata ttattaactc tcattttctt taggatatca aatccattta
     3181 tattaggaag cattaagtct aaaataacta aatcatatat attttgattt actaagtata
     3241 aagcttcttc tccatcttca cacttttcag tttcaaaatg cttgctaagt tcttctacta
     3301 cactttctaa aagtatctta ttatcttcaa ctattaaaac cttcataagt atcccctacc
     3361 tttatttgtc tatcttaact ttatgagatt ttaagtcaat atccacattg gctttttctt
     3421 ttatatcatc atacgtatat cccactattt caacactttt tgcctttgtt gaacacaata
     3481 tatctatatc tgtctcttct gaaggcttta aaatcttata tgatggtaca cgatttactg
     3541 ttaaatattc tccattttca ttaacttctg ctgtcttaag aataatattt cctaaatctt
     3601 ttgatgatga attttttact tttacttgat acgtaattcc ctcatttgat tcatttactt
     3661 ttttaagttc tgatgtagat agcacctcat actgactact attttctagc ttaatattat
     3721 tatttcttat attaatagta ttttctttta aattaacata cacaatctta ccctctgcct
     3781 catattcata tgcataaact tctacaccct ttacaaaagt tttatgccca cattctatgt
     3841 aagcaacttt cccaggcatt aaagtcatat ccaacaaagc cttactatcc gaagatataa
     3901 tttttttatt ctcatcatat tcattatata tcaattcaac attggatata ttaaatgttg
     3961 aattattttc tacctttgtt gatgtatcta ctttatcacc ttcatctata tttataattt
     4021 ctgattttcc tatattcaat acttttgcat ttccctcatc tatttttatc tcatttttat
     4081 cttctgtttg aatatcaact gaatcaggct gttcatcttt ttcacatgct gtcaacataa
     4141 aaataaatat tgttaatata atcattagag aaattgattt ttttactatt ttattcatat
     4201 taagcacctc atatagtagt attttatagt agatttttat actttttcat aaattctttc
     4261 aatgcctgtg taagtatttg atgtttttca aaattcgaat tactacataa tctttcaaaa
     4321 tcattccata tttctttatc tatacgtatt cttgttgttt ttaattcatc tatgtgttca
     4381 tcatctatat gtacatcttt tttattagat acttttttag gctttatatt gtaatctttt
     4441 acttctaaat accaatcata tacttcacaa agcttatcta aatcttcttg agttatattt
     4501 atttttctat ctttcttagg cttaacattt tgttttcgct ttgcattttt taaacctgta
     4561 tcattaaact caatttgttc aacactttta cttaaagcct tttcattaga aatctttttt
     4621 ttggtgctta ctttttttct attacttata gtattctttt tacttttttc atactcctta
     4681 gctttctctt cttccaagtt tctattatcc aaacttttat tataatttaa ttcgtctttt
     4741 aatatgtatt tacctttttc tgacttatat ccttttttat ttaaaaaagt tcttaaagta
     4801 ctttgactaa tctttagatt ttgagctatc tctgcaaatt taaatccttt gctttgaagt
     4861 tctaaaaatt tgtcaattct gtcttcattg ttattcatta atattcctcc gtcaacatct
     4921 tcttgtcttt cttttcatac ttcttattat attcttcttt tctaaatcta ctctatttga
     4981 ttcaattatt ttttgtagca ttttattata agtgaaatca tctaaattat tattttttat
     5041 atagagaaaa gtttcctctt cacatttaat aaaacaaatt gatagcaacc atgccatacc
     5101 cattttaaca taatattctt cactttgtat attattacag tattcaaata tttctttaat
     5161 atgtttatca tcaacataat atattaaaaa cataactaat gcaaatctaa tttcccatgg
     5221 attctttgaa taaacatatt ttttaagtat atcatacata ttttctttat attttttcat
     5281 agactttaaa ccagaacaaa acccatcgca aactgaccaa ttatcgattt taggtataaa
     5341 tctttttaca tagtataaag tttcttcaaa attactattt atattgccaa ttaccatacc
     5401 ttgtagccta acttcttcat agtatgtatc ctctgctact tctaaaaatt ccttccaatt
     5461 cccttggctt atttcttttg ataactttct taaattagga actctaatac ctattatatt
     5521 ttctgttcca ggtataagct ttttattaaa atctctatat tttatatctt ttatagacat
     5581 catatattct aaaagttcat tataatccaa tttattccat tttcccctat caaacatcat
     5641 ttaattgctc cttaattcct tttttcttat tgtaacataa aactctacaa aaaaagcttc
     5701 taactattag aagctttttc aaaaacataa gaaacttttc aattttaaat ttaaactaaa
     5761 ttaatgctaa tctcctacac caaagtctgt attattgtat aaaacttcta tatttggatt
     5821 ttcatccaag aatttatact tactaacaaa ccatttcact aactcttcat ctctttttat
     5881 actagaagaa ttatttataa aaacattcac tgtcgctttt tcaaaagtat ttaggataat
     5941 ctctatatct ttatctatca tctcttttgt ttgtcctttt attccaatca taatacaagg
     6001 tgaatcaaaa tattctttta catcttgtgg agtcttaaaa tttgcatttt tatttaaaat
     6061 attatttcta aaatcattgt caaaggtttc aactccaatt ttaaatacca caggaatttc
     6121 aaaatactct ctcatttcct taagcctatt tttgtaagac caatgacttt ctaaaaataa
     6181 tttttttata ttcttctctt ttataataca ctttattttt tctaaagtat ccttaggtaa
     6241 ttcaaaacag cttccagaat ttatgacttc aagaactcca tatttcccag ttacattttt
     6301 taaaaccctt agatttaatt tattcatctc ttcttctaaa ttagaattat catctatata
     6361 atcacaaaaa gaacactttc cccatataca aggaaatcct ttaagcagaa caatttctct
     6421 ttgattttta tttgttatct cactatatct atccattttc tatctatttt cctccagtca
     6481 atgatacctt taaagttttt ggaactgcat ttaatcctaa attcactaaa acaactgcta
     6541 acagcttatc tccataatca gtaaatactt gggttacaaa cacactaaaa acatctccca
     6601 gtcctaacac tttcaagatt tgcactatat aggatgaacc tgaagaggtc atacctccaa
     6661 ataaaaatgc tgaaattatt gaacttatta tagatgttgg aatagcaaat aaaagtactc
     6721 ctagtggagt ttttaaaccc cttaaaaaat tctttttaaa tgctattcca gcaagtaatc
     6781 cggttgatat ttgaactggt gcaaaataga gagaatacac atcaaacgta attccactta
     6841 ttaaacttcc aaacacacct gtaacaacag cgtatttagg tcctaaaagt gaagctatca
     6901 ttatagttcc tattgaatct aagtatacag gtaatcttaa tcccatagct ataaaagcgc
     6961 ctattatatt tatagaaata ccaaaagcta taagtgtaat agtttttaca catatttttc
     7021 ttttcacact atatcactcc ttccacagag tctataatgt cttcataacc tttaaaaagc
     7081 ctctttaaaa acatgtacat aaatttcttc tcatcaactt catttagaac aattgcatta
     7141 ggattttttt tatagaaatt gaaagaatcc actatagact gacccatagc tattccatct
     7201 tctacaactt ctacatatga ttcaaatcct ttacatatac ttctgtctat aaagtacgct
     7261 actgctagag ggtcatttat cacacagcca attattcctt cctgttccca atggaaatca
     7321 atataaaatc tagttatttc agttatatac tttgccatct tcttatcaag tctatttata
     7381 aactcaataa tattaggagt aagtacaatt tttctagtta catctaaacc taccatgtgg
     7441 atttttttag ataaattctt gtaaacataa tctgctccat gtgggtctac ccaataatta
     7501 aactctgcta ctggagagca atttccatga atcctaaatg ctcctcccat agatacaaac
     7561 tcatcgagat tttcaaatgc tttcttatct ttaataagtg ctttagctat atttgtaagt
     7621 ggtgcaagtg ctattattga tactttttca tgattatgta aagtatttat tataaaatcc
     7681 actgctccat tttttgcttt agctcctaca accttttgat aaaagttttc tccaatacca
     7741 tcttctccat gtgtatcttg agctgttaca agttttcttt ttagtggtgc ttcttctcct
     7801 atatatactg gaatatttag tgaagaacac atttgaagtg tttttagtgc attttctgca
     7861 cctatatttg ctggaacatt tccacaacat gtggtaattc caattacttc tagctctggt
     7921 gagtttaaag ccagaagaat tgccaaagaa tcatcaattc ctgggtcaca atcaattatt
     7981 acttttctct tttccatttt ttatcctcct tgatttgtgg tcgaggagaa ctacacatct
     8041 tttttccact ttcaaaaagg tgtgtctaag ggtattaatt atatactgcc actatataat
     8101 tccttagttt tttatacttg gagttctcga acaagtacag atgtaaaatc atatttttag
     8161 gctacttact atagactctt aaaaactata gactgttaaa aaatttttaa acatctgatt
     8221 taatagctat aaaataaatt ttatagcata aaagaattta ttatatttta atgtatctta
     8281 ttaaagttta tttagaatta tgaaccaact ttaataagat gtggcgcatt taaaccgatt
     8341 attattatac aataatatct taaatattac aattaataac attaatttat tatccataaa
     8401 aatatattaa cttatattat ttaataattt attgacagta ttaaaattaa attatacaat
     8461 aacagtgtaa attaacttaa caccgttaag caaagaggtg attatatgag tatcaaagaa
     8521 agacgtaaaa atgaaaaaga agaaatgaag aaaaagatta tggatgctag catcgaaatt
     8581 ataaaccaac atggatatga aaatttatca ataagaaaaa ttgctacaaa aatagaatat
     8641 tctccaacta caatttatct ttactacaag gacaaagcag agattatcag cgatatgacc
     8701 aataaacttt ataacacagt agaaagtaat gctattgaca ttatgaataa ttgctcatca
     8761 ctaccaatag ataaacaaat catagaaatt atgactagct ttataaaaac tctttctagt
     8821 gaaccagaga tggcaaaagc tatcatgcat agtagaatga atattatatt tgcatctgaa
     8881 aatacaaaca atactcctag caataatggt ataaaaatgc ttgataaatt cctttccatt
     8941 ggaattgaac aaggaatatt caaaaaaaat atagataaaa gctcatggat gcttataagt
     9001 gcattgttag gattcgtttt atgtgttgtt gaaaataaac tatattcttt acataatttt
     9061 tctcaactta tagaaaattt cttagatatt ttagtgggag gactttacaa tgaaaattcg
     9121 caacaaagct actaataaaa agaaaaaata tgtaaaaata tctattgctc tattctttgt
     9181 atttatatgc attattattg taattgtaag caacttaaag ccagaaaact tggtagtaaa
     9241 agatatagat attaataatg ttaaaaatgg catttatact ggaagtgctg ataataactt
     9301 ggtaaaagct actgtgtctg ttgaagttaa taatggtaaa atccaaaata ttaatatctt
     9361 aaaacacgac catcttttag gtaaacctgc tgaaaaaatt acaacaagta ttattaaaca
     9421 acagtcttta gatgtggatg ctataacttc tgcaacttat agcagtaata ctatacgaaa
     9481 agctgtagaa aatgctttaa gaaagggaga ataattatga atactattat tatatattca
     9541 agtaaatatg gttgtacaaa agactgtgca aatatactaa aaaataaact atcagataat
     9601 gtcactattg ttgatattaa taataataat aaaattgagt tatcaaaatt tgataaaatt
     9661 attattggaa gttctatata tgttggttca gtttcaaaaa aaatacaagt gctatgtaat
     9721 gataatgtag agttattaaa caaaaaacaa gttggcattt tcctttgctg tggcttttct
     9781 gaacaagcag ataaatattt aaaatcaaat tttccatcat ccttattaga aagtgcaaat
     9841 gctattggaa tttttggtag tgaagctcga ttagaaaaaa tgaaattttt agataaactt
     9901 attatgaagg ctgtttcaaa aggtaattat gacagtttta gaatatcaca ggataatata
     9961 gataattttt taataaatct taatagctaa aataaaacta attatttcta tgataaattc
    10021 ttttatttat aagtaatatt gctcaaaata gtctttaaac tttatcaaaa ttttattttt
    10081 cttagtgaaa tctaatgaaa ctagctctta atatatgctt tattaatcat aacaaaaaga
    10141 gagtgtctaa tgaactaaaa agttcattag acactctctt tttgtatgaa atcaaatata
    10201 tttttatcat ttcaaaagag aaaaatacac ttgtctctat gtttgacaca ttctcttttt
    10261 ataatataat atctaattta aaacctattt caaatattga catatactta aaccatagac
    10321 ttacaaaatc aattcatatt agcttaaaat ataatctttg ttactcaaat tcctatacac
    10381 aaccattatt tactttgata ctttaaaaca tcttccctat tttttattaa attttataat
    10441 aatagtccta ttaacgcaaa tactataaaa agagtttaac tttatccatt aaataaattt
    10501 aaattaaaac atatagaaat ggaaggagaa caatttatga taaaactaat cggtatattg
    10561 atagttgtaa ttggatttat acttaaaatt gacacattat ttactgtatt attagcagga
    10621 gtggcaacag gaattgttgc aggattagat tttaatcaaa tttttacaat tttaggagat
    10681 tcttttgtct caaacagagg tgtatcatta ttcatattaa cactgccagt aatcggtgta
    10741 ttagaaagat atggtttaaa acaaagagct gtttctttaa tagaaaaatt aaaaagactt
    10801 acaacaggaa aagtacttac tatatacatg atagctagac aaatagctgg tgctctatca
    10861 ataagaatga gtggacatcc tcaatttgta agaccacttg ttaatcctat ggcacaagct
    10921 gctggtttaa gtaatagtga tgaactaaaa gaatcggatg aagaagccat aaaggcatta
    10981 tctgctgcat cagaaaacta tggaaatttt tatggtcaaa acttatttgc aggtagtagt
    11041 ggagtattac ttatagcatc tactctaact caatttggat acaatgtaac tggacttaat
    11101 attgtaaagg ctagtactat tatggcagta atagcacttg tagtggctac aatccagttt
    11161 accttatatg ataaaaaatt aaacaaaagt cataaaaaat agttttattt aggaggaata
    11221 aaaatgcaaa attttataaa catatcattg gaaatctttt acgcactaat gggattttta
    11281 atgattgtta tagcatataa gtcttttact accataaata acaataagaa atatggtaca
    11341 tcattatttt ggatacttat atcactgcca ttcatatttg gtagattgat tccagccaat
    11401 ataataggaa taatattaat attatctagt ttattaacac tttctaaaca agttgtattt
    11461 gcaaagtatg aagaaccaga tgaaaatttt ggaaaagaac aagcagacaa acttaaaaat
    11521 aaaatattta taccatcatt aatattagca tttgctgcag tagtagtagc tatgagcctt
    11581 tctaattttg ataattcttc tcaatttgca ataggtgtgg gttcaataat tgcattaata
    11641 tcagccctta taataactaa agcaaaacct gctacatcag ttcaagatgg ctcaagatta
    11701 cttcaacaaa tgggacctgc tagtatgtta ccacaacttt tagttgcatt aggagcttta
    11761 tttactcagg ctggagtcgg cgaagttatt tcaaccatga tatctggtgt agtccctgct
    11821 gatagcagat tatttggtgt tatagcttat gtattaggta tggttatatt taccatgata
    11881 atgggaaatg catttgccgc attttcagtc ataacggcag gaattggtat tccttttgta
    11941 ttgtctcaag gtggaaatcc tgctataatt ggagcattag cattaacagc aggatattgt
    12001 ggaactctac ttactccaat ggctgctaat tttaatatag ttccagcagc cttgctagaa
    12061 tgtaaaaatg attatattgt tataaaatat caagctccag ttgctttggt attaattata
    12121 gcccacatat tagtaatgta ctttttagga ttttaatata attaggaggt aaaataatga
    12181 aaattttgtt aacaggattt gacccatttg ggggagaact aataaaccca gctcaagaag
    12241 cagttgaaag ggtcaataat aatataaacg gtgcagaaat cataaagata actataccaa
    12301 cagttatgac aaaatctgtt gaagctatag ataaagctat tcaagagcac aatccagata
    12361 tagtaatatc agttggacaa gctggaggta gatttgacat tacacctgag agagttgcta
    12421 taaatataga tgattttaga ataaaagata atgaaggtaa ccaggttata gatactatta
    12481 taaaagaaga tggtgaacct gcatattttt caaaattacc tgtaaaagcc atggtaaaac
    12541 atatgaatga aaataaaatt ccagctagtg tttcaaacac tgcaggaaca ttcgtttgta
    12601 atcatgtgat gtatggaatc ctttatatga tagacaagaa atatcctaat ataaagggtg
    12661 gatttataca catcccatat acaacttcac aggtaataaa taaaaaaaat acaccattca
    12721 tgtctttaga agaaatagta aaaggtttag aattagctat agaagcttgt attatataca
    12781 aagaagatgt aaaagaaatt ggtggtgaaa tttcataata aattttttag tattttataa
    12841 attaaaaata ctaactatta atttaaatat agactgtgag tagattattc aaacaatttt
    12901 actcacagtc tttttatggc atctacagat acaaaattaa ggtctattcc tctctcccca
    12961 ctcacacatt gcatctaaaa ttggaatcag agattttccc ctttctgata aactatattc
    13021 taccttagga ggtatttgag gatattcagt acgaataatc aagttatctg cttctaattc
    13081 ttttaatgat aaactcaaag ttttatagga aatagtattt atataacgtt ttaatgcatt
    13141 atatctgaca acttcaaatt ctacaagaca gtaaagaatt atcattttat atttaccact
    13201 aatcagtgaa agtgtataac taaatcctgt ttcacttaaa tctgtatttg atatacagtc
    13261 tatactcata tattacactc tccttttggt tagtattgaa caaaaatgtg cgtacttgtt
    13321 tttagaataa tttataataa aattatagtg taagaataat tgaaagtcaa ttttataata
    13381 ggaggtgtat ttactaaaaa aagagttatt ttaaatagga ttctaagatt agacatttct
    13441 atataaattg ataatattca tgaaaatcct attttataaa aaataatatc tcttagaaaa
    13501 tctattaaat aaaattcatt ggaggaaatt tataatgaaa gttttattga taaacggtag
    13561 cccaaaccaa tatggatgta cttatacagc actaaatgag attactaaag tattgagtaa
    13621 acacgatatt aaaacagaaa tactgtattt aggtaaagaa gcaatcccag gatgtattag
    13681 ttgcgcatcc tgttttgaaa ctggaaaatg tattcgaaat gataaagtta atgaattgat
    13741 tgaagatctc gataatattg atggtattat tattggttct cctgtttact tttctagtgc
    13801 aactggtcaa ttaacttctt ttcttgaccg tctatttttc atagctggaa gtcgcatggc
    13861 tacaaaatta ggtgcttcag ttgtgtcatg tagacgtggt ggtgcaagtg caacatttga
    13921 ccaactaaat aaatattttt caatcagcaa tatgcctatt gtatcatcac agtattggaa
    13981 tcaggtacat ggctttactc cagaggatgt tatgaaagat gaagaaggat tacagactat
    14041 gcgaactcta ggagaaaaca tggcatggct tcttaagtgt atatcagcag gtaaaaaagc
    14101 tggaattaaa gaaccacaat atgaagaaac aattatgact aattttataa aataaataaa
    14161 aaatgcacta atattaatta aaacaaaaca ctaatttata aataaaataa gtataaatat
    14221 aggataaaaa acactaaaac agaggtattg aattttatat caaacctcta ttttattatt
    14281 acattaataa ccaaaccata tataacatca tatatttcta aataaattat aatattatat
    14341 tctaatgtta ttgaaattac aataactgaa tttctgctca taaatttaaa tattctacaa
    14401 atctcatcta aataacaatt ttaatagacc taaaacaatc ctcatattgt tcattttgta
    14461 ttaaaaatat cacttacaat caaaaaccat ttattaagta tattcctaag atatacaata
    14521 tatatacatg gattggatag tataaataaa ataaatactt catactcttc cctttttctc
    14581 cattgtaaag taacattaaa ggtagagaag caatcatcat ccattggtaa ttttgtaaaa
    14641 tagaattttt taaactataa tctccatttg acattgttgt aaatataaat atacacatca
    14701 aaacataaca aatactcaaa ctgaactttt tacctctaca caaataaaaa ataatcccaa
    14761 gtataatcca aattggtcca ccttctacta aaaatggtac tggtaccaaa atcataaata
    14821 aagtaataac tctgttagtg actttaaaaa gtaaagctac tgtaaatatt cctaaaatta
    14881 taggaatgat tatcatcaat aatcctataa taaaatattt aatttctttc tcttttctaa
    14941 atttctttat gaactctatt gcttgtaaat aaatcgctat taaaaataat gttgaaaaaa
    15001 tgttatttat aattatagct ccttctggat gtgaaaatac attattaatt atttgatttc
    15061 ctatagccat aataacagac caaatataca atctacttat atattttttt ctgttactag
    15121 tgtgataaaa tccttcaaca gccatgaata taaacagtgg tgctgatatt ctgcctaccc
    15181 aatgaaacca aattggtatt tgcattgttt gtggcataaa tgctgctata tggtcaaatg
    15241 tcataaatat taatgctaaa atcttaatag taaacccact tatacctttt ttaaaaaaat
    15301 ccatatcata tctcctaatt ttgttttatc tctataataa tttttgttcc tattccaatt
    15361 tcactactta cataaatttt acctttatga atctctatta tttctttaga aatagccata
    15421 ccaagacctg aacctttatg catctcacct gtattagtac ctctataata ttttttaaaa
    15481 atatgtttca aatcactttt gcttattcca attccattat cttttattat aatttctata
    15541 ttatcttttt ttacaatttc tacacttatc aaagttcctt cgctattatg aactattgaa
    15601 ttaaatataa gattggttat agcccttctg aataagattg aatctacata tacttcaatt
    15661 aaatcttcat tactttcaaa ttcaatattc ctatttttat atctattatc atttaaaata
    15721 tcaattatta tatctctgac taaactgact aaatttattt ttttcttgtc cagaactata
    15781 gtattatttt ttaatcgagt agataaattt aaatcatcaa ctaaatcttt tatatactta
    15841 gacttattat atataatttc tgtatactct cgtatttcat cttcttcaaa ctcataatct
    15901 ttgtcattaa ttatctctgc atacccttgt atagatgcta gaggtgtctt tatatcatgt
    15961 gttatatttg ccagccattc ctctctcaac tcttcatttt cttttctctc aacttcattt
    16021 actcttaaag tatcagctaa catatttata tttttaaaaa cttcttcata tatacctctg
    16081 tccttaagat ataaactata atttccatta gccatagtct caatactcca tagtacgctt
    16141 gaaattggtt ttgtaagttt tctactaaat aaatatccta cacctaaagc taccacgcta
    16201 tcaattacta gtgttataat gataacttta tttaaagttt ttttaatgtt attttgagaa
    16261 taggttaaaa ttattcttga tatatctcta ctaggtatac ctactattat gtttagatgt
    16321 tttttgttta aatatttttc tagtacaaaa ttaacatact ttgtttctat atacttgtaa
    16381 ttattcacca tttgaaaggg tgtatatttt tttggagtat cctttggcac atttactcca
    16441 taaacttcct ctcctaaatc atttaaaacc tgtatccagg aatttgattt ttctaatttt
    16501 tcagctccaa tcttagatag tttaaattct ccatcttttt catataaatc tttttcaaaa
    16561 gaacgtgcaa attcttcagg attatttcca gaatctacaa ctttaaaaaa gctattagtt
    16621 gaaatcacat acaatataga tataatattt ataattacta ctgatatagc tacaaacaca
    16681 attgtaaata taaaatttct agtaatcttc cacttcatat tctaatcttc ctttactgct
    16741 aatttatatc caagaccttt tattgtcaga atatattcag gctttgaagg attatcctct
    16801 aatttttccc taatttttct tatatgaacc attatagtat tgtcaaatcc aacataatct
    16861 tctccccaca ctttatccca tagtttttct ttacttataa tttgattttg attatgtgca
    16921 aataaagata ggatttttag ttcctttgca gacagtccta tagattttcc atcttttata
    16981 agttctgccc tactttcatt taatatatat gggccaaatg atataatttt ttcttcattc
    17041 ttatttagat ttttactttc gttttgaaca tcactaaaat aacttaatct tctcaaatgc
    17101 gctttaactc taaaagccac ttccttaggg ctaaaaggtt ttgtaacata atcatctcct
    17161 ccaatcgcta gtccaagtaa cttatctaat tcttcggttt tagctgataa aaatattatt
    17221 ggagaattgg aggtttttct tatttcttta caaatatcat aaccttcgcc atctggtaac
    17281 ataatatcca atacaatcaa atcaggatta atacttttaa ataattctaa tccttctttt
    17341 ttagtttttg cagtatatat attattaagg ccctcttttt ttaggacagt ttctaaaagc
    17401 ttcaatattt ctacttcatc atctattatt aaaacacgat tttccataaa caacctcctg
    17461 taataattta catatatatt agcatatatt ttatctaaaa ttcatttatc ttatttttaa
    17521 ttaaactaaa atataaataa aattactatt atttttagtt aaaacctttt gcttgatgta
    17581 aaattttcat atgttatttt agttaacact atcattaata taattgagat tactatagaa
    17641 aaaactatca aaatacttgg tacattatac ctaaccattt gttggctcat cataatccaa
    17701 tttggagtca ctgacataaa ttttatagct ttatctggta taaaacctac actaaagtat
    17761 ggaattgcca ttatcaatcc acctagtata aatggaatta cagccgattt aaatatagat
    17821 gaaagtgtta agcctatttg tgcaaatata aaagaagcaa aaatagtagt caatataaca
    17881 attatataca tttgtattac tgtaagattt aaattactat ttccaaagta ccataagtta
    17941 tgaatcggtt catttaaatt tttaaaatca aaaaaactaa aacctctaat tcctactaac
    18001 aatataaaag aaatattaat aatagataaa attaatccat tagctactag ttttgataat
    18061 actactgatt tttttccctt tttagttgta tttattaaac ctgtcatatt agaatcatat
    18121 tctttaatgt aatttccaga agttattata aacattatca aacctattag aggtattgag
    18181 ccttctatct tgtaaaatat atctgcataa gtatctaaaa catcaaactt tctaatcaga
    18241 agaaatccca aaaatattaa aaacagccaa attatccaaa cactctttga ttttaatatt
    18301 tttatcattt cagctttaac taaatacatc atttctacta cacctccctt gcatcttcca
    18361 aatcaaaata aaacatgtat acatcttcga attttgcttg aactggttgt atattatccc
    18421 cacttggaat atcctctgaa acaattctaa gtactactga atcaatatca ctttgaatac
    18481 ttacaacctt atatttagat tgtatatcat caacttcaca ttcattttta acttgaacat
    18541 tatatacttt attttccatg tgacttaata tgtctttgtg tgttcctttc attagaagtt
    18601 tgccatcttt aatcattatt gtttcctttg caactgattc tatatcggaa attatatgag
    18661 tagaaagtat tattatctta tctctagaca tttttgctat taaattccta aatctagctc
    18721 tttcttgtgg gtcaatacct gctgttggtt catcaaagac tattatctta gggtcgttta
    18781 gtaaaacttg tgctatccca actctcctct tcatacctcc tgaaaatttt cctatagact
    18841 tatttctcac atcgtaaaga cctactaaat ctaataattc tcctatctta ctatttaaat
    18901 attttcccct taaacctttt aaagctccaa tatactgtaa aaattctttt gctgtaaaat
    18961 ttttataaac tccaaattct tgtggcaaat atcctaaaat atttctataa ttagcatcca
    19021 ttttaaatat atcctcacca ttataaagta tctgaccttc atcaggtttt atcaacgttg
    19081 ttatttgttt cattaatgta gattttcctg ctccatttgg tcctagaaga ccataaactc
    19141 cattttccag agttactgta atcttgtcgt ttgctacttt tttaccatat atctttgaaa
    19201 gattttctat ttctaattta ttcatcttaa ctcccctttt atctacttat ataatctgat
    19261 tttattacta aaaaacataa tgacatagca cctatcaata ttgccatcaa aagtcctatt
    19321 gctatattga caatgtctat attcacttta aatatcataa tatcacctag ataatttcct
    19381 attattttat taaaaccaaa tatcaaatca taataattta catttgatac aactgaaagt
    19441 aattttttag gtaatatatg gattaccgac attatcttag ttatagatat aataaatata
    19501 aaaaaactta ttgaagatat tgaagattta cttataaaag acccaaatat ggctatcatg
    19561 gcccatcctt caaacatcaa aagctctatg cctatattta atattacata ctttgttata
    19621 cttaaattag ttttcaatgc aactacattg tctactattc taaaagattc taagtctcca
    19681 ttaacaggta ctccttgttg catacaagtt ataataaata ttcccattaa ataaacagaa
    19741 taaattacta atggaactaa taaagcaaca aatattttag actttaatgc ttttttcttc
    19801 tcttttgatg atagtatgat atcttttacc gaagtaacga cttcatctac gtaaatattg
    19861 gatataatta tagatataat gattaacatt cctacattta taagtgaatt tgtaactcta
    19921 tagttaaata ctttccaaaa actaatatcc ttatattcat tcacacttag attatcaagt
    19981 tttttctttg acattaattc aattttttta gaaaattcac ctgtatctgt ttgatttgaa
    20041 aactgcttta aaacattaat cttattttga aatttctcat tacctatgtc gataccactt
    20101 ctcgtatctt ctatacatcc tttttcttta tctatataat aacgttctgt ttctaaaaca
    20161 ggcgtaataa agatactaat cattagtatt aacactaata tccctagaga tataaacata
    20221 ctcttagatt tagccaattt ttttatttcc caatttacca atatatttct cctcctaaat
    20281 cattttaatt acacatatat attaaaactt a
//
LOCUS       DAMJWP010000050        20274 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.051, whole genome shotgun sequence.
ACCESSION   DAMJWP010000050 DAMJWP010000000
VERSION     DAMJWP010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20274
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.051"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(480..2084)
                     /locus_tag="PC700_001546"
     CDS             complement(480..2084)
                     /locus_tag="PC700_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2580860.1"
                     /translation="MKEKIYYNGNIITMEDSICGDAILIKDKIIKKIGTKEEVFALKN
                     KDTEIIDLQGKTLMPSFIDSHSHLIAFATTLKLVPLEDATSFKDIVKKIQDFKESNNI
                     KKGDWIIGFSYDNNFLEENKHPDKSVLDSASSENPILISHASGHMGAANTLGLKQLGV
                     TNETRDPEGGHIGRVEGSEEPNGYLEENAFFNVASKIKQPSSNEIFNSIEKAQNIYLS
                     YGITTAQEGLMEENQFNILKAMANQNKLKMDVVGYVNLKKSKSVLDNNREFIKKYINR
                     FKVGGYKIFLDGSPQGKTAWLSRPYENSDDGYCGYPIYKDEEVEKFIDISLKEKMQLL
                     THCNGDAAADQLIDAFEKVLNLKEQSSENNIRPVMIHAQTVRADQIDDMKVINMIPSY
                     FVAHTYYWGDIHIKNLGEDRAFKISPLKTTIEKGLIYTLHQDTPVIAPNMLETVWCAV
                     NRITKKGIQIGENEKISPLDALKGVTINAAYQYFEEDKKGSIKEGKLANLIILDENPL
                     TIDPMKIKDIKVLQTIREGEVLYSLK"
     gene            2470..3396
                     /locus_tag="PC700_001547"
     CDS             2470..3396
                     /locus_tag="PC700_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HDF2580861.1"
                     /translation="MSKENKNVRIAVVQASPVIMDLEKTVEKALGLIKEAGRKGANIV
                     VFPEAFIPAYPRGLSFGFVVGSRTMEGREDWKRYYDNSVPVPSATTDLLGKAAQEAGV
                     YLSMGITERDGNDINCTLYCTNLFFSPEGKLIGKHRKLKPTGTERCIWGEGDGSTLTV
                     VDTPYGKMGSLICWENYMPLARTVLYAKGVKLYIAPTADSREEWQATMKHIALEGRCF
                     VIGCNQYVEKNMYPTDLNYYKELDAEPEIMCPGGSCIVDPFGKYVAGPIFNKEEMLIA
                     DLDLEKIVLSRLDFDSEGHYSRPDVFELIVHE"
     gene            complement(3516..4514)
                     /locus_tag="PC700_001548"
     CDS             complement(3516..4514)
                     /locus_tag="PC700_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HDF2580862.1"
                     /translation="MNKKFKSLYITEEEGNFRRNIVEREVDTLPEGEVLIKVKYSSLN
                     YKDALSANGNKGVTRKYPHTPGIDASGIVESSSCDDFKIGEEVLVTGYDLGMNTSGGF
                     SEYIRVPSSWVVKLPKNLSLKEAMDYGTAGFTAALSVYKFIDSVDKNMGDVLVTGGTG
                     GVAVIATKILIKLGYSVVVSTGKLEEQKEVLLNLGVKDVIHRSEVDDNSGRPLLRPRW
                     AGVIDTVGGNTLATAIKTTNYCGAVTTCGNAGGVDFTSSVYPFILKGITLYGIDSVDC
                     PMNLRLKIWDLLSNEWKLDDLDDMVNVVSLEELNDSIDSIMTGKHVGRTVVDLEKI"
     gene            4618..5055
                     /locus_tag="PC700_001549"
     CDS             4618..5055
                     /locus_tag="PC700_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2580863.1"
                     /translation="MEDKYIVHFISKTKASMIKFIENKLSKNGLGELIPTHGNILTAL
                     YENNGILTMKEIAAKIGKDKSTVTVLVNKLINLGYLERQKCTNDKRITYIKLTKKALL
                     IEDTFNSISTQVKETAYHNITENEKQEFLRILKKINDNFKNAD"
     gene            5141..5728
                     /locus_tag="PC700_001550"
     CDS             5141..5728
                     /locus_tag="PC700_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HDF2580864.1"
                     /translation="MNLKDSKTKENLLRAFAGESQARNRYNISASVAKKEGLYIIESL
                     FNYTADQEKSHAKQFFSKLKEFAGENISISGAYPVDLYDKTAQHLKAAQHNELQEWDD
                     VYKNFGKTAREEGFESIAKLFENIAEIEKVHGDRFGRYATDLEEGTLFRRNEDIQWIC
                     THCGHIHIGKEAPKACPVCLHPQGYFLDFKNSLFE"
     gene            complement(6028..6648)
                     /locus_tag="PC700_001551"
     CDS             complement(6028..6648)
                     /locus_tag="PC700_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbD family protein"
                     /protein_id="HDF2580865.1"
                     /translation="MEELISKKDLLSKTNISYGQLYRWKRKNIIPEEWFIKKSAFTGQ
                     ETFFPRDKILERIELILSMKEETSLDDIANMFMKKDINKEFDIDFILSKEVICNYTKE
                     IFQNLYKKEQSIGKKELLILSIIEKFLVKSIITFEELKLIVNIIEENFTSIYNESGKI
                     YLFRKFGVPFVVGCLDYKKVKFEKDVVKIIEIDLTKEINDISIKLS"
     gene            complement(6656..7423)
                     /locus_tag="PC700_001552"
     CDS             complement(6656..7423)
                     /locus_tag="PC700_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HDF2580866.1"
                     /translation="MEKGSIKILGEGFISSGEYENIKIVGSSKSKGNVKSNEVKVLGD
                     AKFEGDINIKNFVVNGKTIIRGNLKAERVRVNGQLNIEGDAEIDDLVITAQQKVDGKL
                     KCNNVTIRGQLSVNENMYAQDIKIYGEMRNKSNIECEDIKVYGVIKCDGLLNGENIYV
                     YSGGGSYCKEIGATNIKIGRSKDAFSLIKIINIFSGKFKCNLIEGDTIELEDASIKDI
                     RGKSISLVDNCDVGNIEYSDNLSVSDNSTVKNSIKVG"
     gene            complement(7464..8285)
                     /locus_tag="PC700_001553"
     CDS             complement(7464..8285)
                     /locus_tag="PC700_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580867.1"
                     /translation="MNEELSSLRFDGVGKCKGGIFDNVNISGTGKIEGSVKCSKFDSS
                     GSAKVFGTTECNEFFTSGLSKVEGNILASKVEISGLLRCTGNINSVEIDTSGIINVEG
                     SIKSDIIHGEGYLKANENIDCENINLSGILECNGFLNCEEVNISLNGTSNFNEVGASS
                     INIKKGHGDYCKKFRMLEKKHINFEKVSSNLISLGLLSIRKYTESKLFANIIEADEIS
                     LENSEVKVVRGKNIKIGKGCYVESLEYSESIEIDEDSIVGEIKNISEKIKLKKDK"
     gene            complement(8642..10552)
                     /gene="phnW"
                     /locus_tag="PC700_001554"
     CDS             complement(8642..10552)
                     /gene="phnW"
                     /locus_tag="PC700_001554"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HDF2580868.1"
                     /translation="MKKIYGEKIKAVVFDWAGTTVDYGCFAPLNVFIEIFKRRGIDVT
                     MEEARKPMGKLKIDHIREMCEMDRIKNLWSDKFGKVPTEDDVNELYAEFEPMLFETLE
                     EYTTPIPHVVETIEKLRKNGLKIGSTTGYTREMMNIVEPNAAKKGYSPDFLVTPSEVS
                     QGRPYPWMCYKNAEALGVSPMSSMVKVGDTISDVKEGVNAGMWSVAVIKGSSELGLTQ
                     EEVENMDKEELKAKMSIVSKKFKEAGAHFVIETMAELEDILIKIENETIKSDFVPEND
                     YILLTPGPLSTTKSVRASMLKDWCTWDVEYNNLVQDVRRRLVSLATQNTDKYTSVLMQ
                     GSGTFSVEAIIGSTISKDGKLLVIANGAYGKRMKDICNYLNIEFVDCTFKDIEAVDLN
                     VVENLLKENKDITHISMVHCETTTGRLNPIQEVGKLAKEYNKIYIVDAMSSFGGIEID
                     VEDFNIDFLVSSSNKCIQGVPGFGFIIANKEKLSKCKGIAKSLSLDVYAQWETMEKNN
                     GKWRFTSPTHVVRAFYQALLELEEEGSVEKRYARYKENQFTIASRLKSLGFDTLVNDN
                     AQSPVITTFLYPKNAKFEFMEFYTYLKDNGFVIYPGKLTDIDTFRIGSIGEVYPTDME
                     RLADVIEKFINR"
     gene            complement(10831..11616)
                     /locus_tag="PC700_001555"
     CDS             complement(10831..11616)
                     /locus_tag="PC700_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDF2580869.1"
                     /translation="MSLNSIDRKKEILKLLDSKGKVNTKELVKRLEVSSETIRRYLEE
                     LENENKLKKVYGGAIKINYGVIEPEHIKRNNTNLDKKQSIAKYAAKFVNDNESIIIDE
                     GTTNLQIVDYIISVNNLKIITNSYPVLSKLISYENQGLFDGEVIFIGGKVNLKHQRTA
                     CPMSIDTMKNLYVDKAFISSEGILDSFGISSIDQNKALLSKEYIKNSKESFVLCDSSK
                     IGIASMYKIADLEELDFVICDVEPPNEWKDKLNEINITWIEAK"
     gene            complement(11833..12063)
                     /gene="dltC"
                     /locus_tag="PC700_001556"
     CDS             complement(11833..12063)
                     /gene="dltC"
                     /locus_tag="PC700_001556"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HDF2580870.1"
                     /translation="MQETVIEIFEDVLGTDEIREDLDLNLFETELLDSLAIIEVLLEI
                     ENRLGIELQPTDLERKDMSTVNNLVKFLETRK"
     gene            complement(12082..13230)
                     /gene="dltB"
                     /locus_tag="PC700_001557"
     CDS             complement(12082..13230)
                     /gene="dltB"
                     /locus_tag="PC700_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HDF2580871.1"
                     /translation="MIFSQYGDYFYLYILLLTSIPAVILGLMGKNIKYYGMLASLFMI
                     FLIVGIDVQLKYLVIFIILEVIIVKGYEYVRRKTKNKYIYWGFLFASMLPIIINKISP
                     VTSFGIIGFIGISYLNFRTIQMVIEIYDGAIKEVKISKMLYFMLFFPTLSSGPIDRSR
                     RFEQDLEKHISRKEYIEEYLLPGIKNIVMGVGYKFVIAFLINTYWVSRIPKDMSFINI
                     LSYMYAYSLYLFFDFAGYSLFAIGTGYIFGIQVPINFDKPFISKDMKEFWTRWHISLS
                     RWFGDYIFSRFVMSSMRKKRFKKRTTAAHVAQMITMITMGFWHGLTWYYVAYGVYQGL
                     ALVLTDIYQRKSKFYKKHKKDKWFERVQIFITFHIVCFGLLIFSGYLA"
     gene            complement(13230..14744)
                     /locus_tag="PC700_001558"
     CDS             complement(13230..14744)
                     /locus_tag="PC700_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase"
                     /protein_id="HDF2580872.1"
                     /translation="MKIIEGIKKYSNTDRTALMCNGDKLSYKDLNEYSDAISVFLKDV
                     YKEEDTPIVIYGNKENMIMACMIGALKSGRAYVPLDISFPIDRVFEVTKEIKPKVIFN
                     FSDERNFGDINVIDMDKLNDIINEYQGKSLDKENWVKDDENAYILFTSGSTGKPKGVQ
                     ISSNNLDSFSDWISPYLNIDGSEKVIMNQPAYSFDLSVTTIYPGLIHGATLFSISKDV
                     LADYKELFRQFSISDIAVWVSTPSFAGVCITEKEFNSKMLPNLESMIFIGEALSKNLT
                     KELMSRFPNTRIINGYGPTEATVGVSVNDMTQKAIDDEKSLPVGYPMSNCKIKILDED
                     GNELKENEKGEIIIIGPSVSKGYFNNKEKTDEVFFYDEIDGVKWRAYKTGDMGYLLDG
                     NIYYCGRKDFQIKLNGFRIEIEDIENNLRKVHNVKNAVVLPVYKDEKIAYLKGIVELN
                     EKNDLSNIKNGMIIKKELGKYIPSYMIPRNISIISEFPTNINGKIDRKKLMEEI"
     gene            complement(14865..16055)
                     /gene="dltD"
                     /locus_tag="PC700_001559"
     CDS             complement(14865..16055)
                     /gene="dltD"
                     /locus_tag="PC700_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HDF2580873.1"
                     /translation="MRKLIYFITPFIIGVVFLFGLDKFLDSKTDELLREKNLLPIMDD
                     TLSDIKDKGVTANNHFLREKDIMILGSSELSNSTKQHPKYYFNTNRSKNKVFAIGRAY
                     TQTLQDAAILGSMNPNIDNKKVVLLISMQWFMEKDGVTSHHYQSRFSPIQFYRFLDNP
                     KISKQNKIEYAKKSSKLLWGSDEYKAEALYAKLYEPKTFPEKAEKVLLEPYFQGRKYC
                     IALKEKGILYKRLIKLDKKRATKRKRPINWSYERKKAIEDAKKRVGKNPLNIDKYYYK
                     EHFKDGIEQYKGRDKDVNLLTSKEFENYKLMLNVCTDLGINPVVVLIPSMDKFYNLTG
                     ISEKERNQYYDKAQKIAESKGFEVLNLKDKGSDKYYLRDVMHLGTKGWVDVCERLFKI
                     FKEQ"
     gene            complement(16343..17362)
                     /locus_tag="PC700_001560"
     CDS             complement(16343..17362)
                     /locus_tag="PC700_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDF2580874.1"
                     /translation="MMGKIRFGIVGTSNIANIFLRAASRVKDFELVAVYSRNLEKARE
                     FGTLHGASIFFDDLEQMAKSKDIDAVYIASPNALHSKQAITCLKHKKHVLCEKSLASN
                     LKEVKSMIKTAKDNNVLLMEAMRITCVPNFKAVKENLYKIGKIRRFFGSYCQYSSRYD
                     KYKNGIIENAFKKELSNGALMDIGVYCIHPMVNLFGAPKTVKAVSHILQTGVDGEGSA
                     VFQYDDMDAIIQYSKVADSYIPSEIQGEEGSIIIEKLNLFEKAIIKYRDGREEDISIP
                     KEKAEEKPREIEGMYYELVEFISLINNNKIESNINSHENSIIVMEIMDEIRRQSSIVY
                     PADSI"
     gene            complement(17538..18077)
                     /locus_tag="PC700_001561"
     CDS             complement(17538..18077)
                     /locus_tag="PC700_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HDF2580875.1"
                     /translation="MDNVGSLLYFLFNFFLYGFIGWIIENLYSYYKQGHFQEDGFLNG
                     PFKPMYAIAMSIIIEVSRFIDPNNIFALLLLCLVVPTVVEYITGILMKVYFNKVYWDY
                     SDYKYNSRGIVCLKFSVYWTILTFIGVRYFQVYIVDSLYSLIIPYWPILVTILLVTLF
                     VDEILTIKFLRGNKDIVER"
     gene            complement(18178..18858)
                     /locus_tag="PC700_001562"
     CDS             complement(18178..18858)
                     /locus_tag="PC700_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDF2580876.1"
                     /translation="MQKVEGIIFDMDGVLFDSERISLEFWMETFEKYGYTMTKEIYTS
                     VMGRNRKGIIEGLTDIYDSSVPIIDLYDEKTKNMIEFMERKGAPIKLGVNELISFLKE
                     NGYKMAVATSTKRERAVKRLAKANLKDYFDAIVCGDDVVNSKPNPEIFLKAAKKINVN
                     PQNCIVIEDSPMGVEAAYNGGIRCINVPDLKEPDEQIKSQSHKILENLLEVREYLKSL
                     NSKECHNL"
     gene            complement(18887..20176)
                     /locus_tag="PC700_001563"
     CDS             complement(18887..20176)
                     /locus_tag="PC700_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDF2580877.1"
                     /translation="MRELLKVTLIVMLVLINAFFVACEFAMVKLRSSRIDTMITEGNN
                     NAKRCKIIKDNLNSYLSACQLGITLCSLALGWMGESTVKELILPIVSVFNLSESIIYT
                     ISISISFLIITMMEVVIGELVPKALALYNTEKIMLSTSFLLVGFYKLTYPIIYFFNLS
                     TDLFLKPFGYSQADEVDEPHTGDEIRLLVEESYKSGLIDESEQRLVDNIFEFEEKKIR
                     EIMVPRTDMVCIYESDSEEKILAILKEEGVTRYPVCRKNKDDILGFVHIRDLYNQKIN
                     ENKIELEEILRDIIYISENLTIDKALERIRKEKLQLAIVVDEYGGTSGVVTIEDILEE
                     IVGEIQDEFDKEETHIKKIGAGSYLVDGTESINYINKYFGLDIEHDGFDSIGGWISYM
                     LGSNIKVNQSVTFENYNFTILELDKLRVVKLIIKSVI"
BASE COUNT         6686 a         3492 c         2085 g         8011 t
ORIGIN      
        1 atttggatag aattattttt taaattagaa ttattttaat gaatgcttca agtaataggt
       61 atgaaattga atcttttaga actgcttata aaaaatactc aaagaaaaat agtaattttc
      121 tcagattcac aatatatttt atatgaaatg ttataaggta atctatttaa taaatttaaa
      181 tttgctatta tagatttagt tccaaagtct tactgcaaag taatatacat agttttttaa
      241 taaactaata agtgcaatta ataactactt aattgtttaa tatagtacat agataacttt
      301 aataaatcga cagttattta aaaaggtaga atcaataaaa ttatacttat aaatcttcaa
      361 tacaataatt aaaatttaat aaaaaatata attgttgatt taactgtaat cttatttaat
      421 tctacctaac ttattatata aaactatttt ttatagatta taaaatatct aactttcatc
      481 tacttcaaag aatacaatac ttctccttct cttatagtct gcaatacttt aatatctttt
      541 attttcatag ggtcgattgt aagtgggttt tcatctaata ttatcaaatt agctaattta
      601 ccttctttaa ttgaaccttt tttatcttct tcaaaatatt gatatgctgc atttatagta
      661 acacccttta aagcatctaa tgggcttatc ttttcattct ctcctatttg tatacctttt
      721 tttgtaattc tatttacagc acaccatact gtttccagca tattaggtgc tattactggt
      781 gtatcctgat gaagagtgta aattaatccc ttttctatag ttgttttaag tgggctaatc
      841 ttaaacgctc tatcttctcc aaggtttttg atatgaatat caccccaata atatgtgtga
      901 gctacaaagt atgaaggtat catatttatt actttcatat catcaatctg gtctgctctt
      961 actgtttgag catgtatcat aactggtctt atattatttt cactactttg ctcttttaag
     1021 tttaaaactt tctcaaacgc atctatcaac tggtctgctg ctgcatctcc attacaatga
     1081 gttaagagtt gcatcttttc ttttagtgaa atatctataa acttttctac ttcttcatct
     1141 ttgtatatag gatatccaca ataaccatca tctgaatttt catatggtct actcaaccat
     1201 gctgtcttac cttgtggaga accatctaag aaaatcttat aacctcctac tttaaatcta
     1261 tttatatatt tttttataaa ctccctatta ttatctaata cagacttact ttttttcaaa
     1321 tttacatatc ctacaacatc catttttaat ttattttgat ttgccattgc ttttaaaatg
     1381 ttaaattgat tttcttccat taaaccttct tgagctgttg taataccata gcttagatat
     1441 atgttctgtg ctttttcaat tgaattaaaa atctcatttg atgatggttg ttttatttta
     1501 gatgctacat taaaaaatgc attttcttct aaatatccat ttggttcttc actgccttct
     1561 actctaccta tatgtccacc ttctgggtca cgtgtttcat tggtaacacc aagctgtttt
     1621 aaccccaatg tatttgcagc tcccatatga ccagaagcat gtgaaattag tattggattt
     1681 tctgaagatg cactatcgag tacactctta tctggatgct tattttcttc taaaaaatta
     1741 ttatcatatg aaaatccaat tatccagtct ccttttttta tattattcga ctctttgaaa
     1801 tcctgtattt tttttactat gtctttaaaa ctggttgcat cttctaaagg aactagcttt
     1861 agagtagtag caaaagctat caagtgacta tgtgaatcta taaaagatgg catcaatgtc
     1921 ttaccttgta aatctataat ttctgtatct ttatttttca aagcaaatac ttcttctttt
     1981 gttcctatct tttttattat tttatctttt ataagaatag catctccaca tattgaatct
     2041 tccattgtga tgatatttcc attgtaataa attttttctt tcataaaatt ctccttacgt
     2101 tgtctacttt aataaatttt tttcattatt ttctgcttaa ttatattatc ttctttataa
     2161 actttttctt tagagtttaa tcatataaaa gttttatttt taattattgt ttgccaagaa
     2221 gtaaatacta ttcattgtaa ataagacaaa ttaataggct ttatactaat tgaaaaaata
     2281 ttataagttg ttaaatttaa atatctaaac tcaattatat ctgtacaatt gtaatttttt
     2341 aaatcatgat gtattatttt aaatatacag ttacattatt attcacaata taagtatctt
     2401 agttctcaaa taacatctca tgtaatctat tcaatgcaaa aaatttaaat attttttagg
     2461 aggtttttta tgagcaagga aaataaaaat gtaagaattg ctgttgtcca ggcgtcacca
     2521 gtcataatgg atttagaaaa aactgttgaa aaagcattag gattaattaa agaagctggt
     2581 agaaaagggg caaatatcgt agttttccct gaagcattca tacctgcata tccaagaggt
     2641 ctttcatttg gatttgttgt tggaagtaga acaatggaag gtagagaaga ttggaaaaga
     2701 tactatgata attctgtacc agttcctagt gctacaacag atttattagg aaaagcagca
     2761 caagaagcag gagtatatct atcaatgggt ataacagaaa gagatggtaa tgatataaat
     2821 tgtacccttt attgcacaaa cttatttttc tctccagaag gtaagctaat tggtaaacac
     2881 agaaaactta aacctacagg aacagaaaga tgtatttggg gagaaggaga tggaagtact
     2941 cttacagtag ttgatactcc atatggaaaa atgggttcgt tgatttgttg ggaaaattat
     3001 atgccattag ctagaacagt tctttatgct aagggtgtaa aactttacat agcccctact
     3061 gcagattcaa gagaagagtg gcaagcaact atgaagcata ttgctttaga aggaagatgt
     3121 tttgtaattg gatgcaatca atatgttgaa aaaaatatgt atccaactga ccttaactac
     3181 tataaggagc tagatgcaga accagaaatt atgtgtccag gaggaagttg tatagttgac
     3241 ccttttggaa aatatgtagc aggtcctatt ttcaataaag aagaaatgct aatagctgat
     3301 ttagacctag aaaaaatagt acttagtaga ctagattttg attcagaagg tcactattca
     3361 agaccagatg tatttgaatt aattgttcat gaataatctt tttacacaaa catctacata
     3421 aattttttta cataaaaaaa tgagatactc ataagtaaat ttcaagtatc tcatttttgc
     3481 ttaaaaccta taatcctaat tataaattta ttcgcctata ttttttctaa atcaactact
     3541 gttcttccaa catgttttcc tgtcattatc gaatctatac tatcatttaa ctcttccaat
     3601 gatactacat ttaccatatc atctaaatca tctaatttcc attcatttga taataaatcc
     3661 catattttta atcttaagtt cattgggcaa tccacagaat ctataccata taaagtaatt
     3721 ccctttaaga taaatggata tactgaactt gtaaaatcaa ctcctcctgc attaccacaa
     3781 gttgttactg ctccacaata attagttgtc tttattgctg ttgctagagt atttcctcct
     3841 actgtatcta ttactccagc ccatcttggt ctaagcagag gtcttcctga gttatcatcc
     3901 acttctgacc tatgtataac atctttaaca cctaagttta gtaatacttc tttttgttct
     3961 tctaattttc ctgttgaaac tacaactgaa taacctaatt taatcaatat tttagttgca
     4021 attacagcaa ctcctccagt accaccagtg actaatacat ctcccatatt cttgtctaca
     4081 gaatctataa atttatacac agataaagct gctgtaaacc ctgctgtacc ataatccatt
     4141 gcttctttta gtgaaagatt tttaggaagt ttaactaccc aactagaagg aactcttatg
     4201 tattctgaaa aaccaccaga agtattcatt cccaagtcat aacctgttac taaaacttct
     4261 tctccaattt taaaatcatc acaactacta gattctacta taccagatgc atcaattcct
     4321 ggtgtatgag gatactttct cgtaacaccc ttattaccat ttgcagaaag tgcatccttg
     4381 taatttagag aagaatattt tacttttatt agaacttcgc cttctggcaa tgtatctact
     4441 tctctttcaa ctatatttct tctaaaattt ccttcttctt ctgtaatata tagtgattta
     4501 aattttttat tcataaattt cctcccaata catttagttt tatgttaaac taattatagt
     4561 ttataaatag ttttatgtca aactaattta aatagattaa agattggaga ataatttatg
     4621 gaagataaat atattgtaca tttcataagt aaaacaaagg ctagtatgat taaattcatt
     4681 gaaaataaac tcagtaaaaa tggtttaggt gagttaatac ctactcatgg aaatatttta
     4741 actgcacttt atgaaaataa tggaatactt acaatgaaag aaattgcagc taaaatagga
     4801 aaagataaat ccactgttac agtgttggtt aataaactta taaacctagg atatttagaa
     4861 agacaaaaat gtacaaatga caaacgtatc acgtatataa agcttacaaa aaaggcactg
     4921 cttatagaag atacttttaa tagcatctca acacaagtta aagagacagc ataccataat
     4981 attacagaaa atgaaaaaca agaatttctt agaattttaa aaaaaattaa tgataatttt
     5041 aaaaatgctg actaaattga gcataattat atctcctatt ggaataataa ataaaaagaa
     5101 tcaaatattg taaaaattta ttatgataga gaggtagaat atgaatttaa aagatagtaa
     5161 aactaaagaa aatttattaa gagcttttgc aggtgagagt caagctagaa atagatacaa
     5221 tatttctgca tctgtagcaa aaaaagaagg attatatata atagagtctt tatttaatta
     5281 cactgctgac caagaaaaat cacatgcaaa acaattcttt tctaaattaa aagaatttgc
     5341 tggtgaaaat ataagtatat caggagcata ccctgtagat ttatatgaca agacagcaca
     5401 acaccttaaa gctgcacaac ataatgagct tcaagagtgg gatgatgtat ataaaaactt
     5461 tggaaaaaca gcacgtgaag aaggatttga atctatagct aagttatttg aaaatatagc
     5521 tgaaatagaa aaagttcatg gtgatagatt tggtagatat gctactgatt tagaagaagg
     5581 tactttattc agaagaaatg aagatataca atggatatgt acacattgtg gccatataca
     5641 tataggtaaa gaagctccaa aagcttgtcc tgtatgtctt catccacaag gatatttctt
     5701 agattttaaa aactcattat ttgaataaac taaggcaata attctattta aacaatttac
     5761 tatatttata tcaagtattt ttataaatac tgtattatct taataacaat aaaatgaacc
     5821 attgatatta ctataagtag aaaatgtagt ttaaatacaa tttatagtaa ttcatcatgg
     5881 ttcattttat tatttactaa tccctataac ctacttaaat ctatctttaa cttttacata
     5941 ctcacataaa atcttatctt taaactaaat atttaaataa agtgtatttt aagtagatta
     6001 ttataataca ttatataaga taatacacta tgataacttt atacttatat catttatttc
     6061 ttttgtcaag tcaatctcaa taatcttaac tacatctttt tcaaacttta cttttttata
     6121 atccagacat cctactacga atggaactcc aaactttcta aacaaataaa tctttccact
     6181 ttcattgtat attgatgtaa aattttcttc tattatattc actatcaatt ttaactcttc
     6241 aaaagttatt atagatttaa ctaaaaactt ctctattatg ctcaatataa gcagttcttt
     6301 tttacctatg ctttgctctt ttttatataa attttgaaat atttctttgg tataattaca
     6361 tataacttct ttactgagaa taaaatcaat gtcaaattcc ttattaatat cctttttcat
     6421 aaacatattt gcaatatcat caagagaagt ctcttctttc atagacaata ttaactctat
     6481 tctttctagt attttatctc ttggaaagaa agtctcttga cctgtaaaag ctgatttttt
     6541 tataaaccat tcctctggta ttatgttttt tcttttccat ctatataatt gaccataaga
     6601 tatattagtt ttgctcaata aatctttctt agatattaat tcttccataa cttttctagc
     6661 ccacctttat actgtttttt acagtagaat tatctgaaac tgataaatta tcactgtatt
     6721 caatatttcc aacgtcacaa ttatctacta gagatatgct tttgcctcgt atatctttta
     6781 ttgatgcatc ttctaattca atagtatctc cctctatcaa attacattta aactttcctg
     6841 aaaaaatatt aatgatttta attaatgaaa aagcatcttt gcttcttcct atttttatat
     6901 tagttgcccc tatctcttta caataagaac ctcctcctga ataaacatat atattttctc
     6961 catttaaaag accatcacat tttattactc catatacctt tatatcttca cattctatat
     7021 ttgatttatt tctcatttcg ccatagattt ttatatcttg tgcatacata ttttcattta
     7081 cacttaattg tcctcttatt gtaacattat tacatttaag tttgccatcc actttctgtt
     7141 gagcagttat aactaagtca tctatttctg catctccctc tatattaagt tgaccattca
     7201 ctcttacacg ctctgcttta agattgcctc ttataatagt ttttccgtta acaacaaaat
     7261 tttttatatt gatgtcacct tcaaattttg catctccaag gacttttact tcgttacttt
     7321 taacatttcc ttttgattta gaacttccca cgatttttat attttcatac tcaccactac
     7381 ttataaagcc ttctccaagt atttttatag accctttttc catattatta cctcatttta
     7441 aattttattt ttaaacctat ttattactta tccttcttta attttatttt ttcacttata
     7501 ttctttattt caccaactat tgaatcttca tcaatctcta tactttcact gtactccaaa
     7561 ctttctacat aacatccctt gcctatttta atattttttc ctcttacaac tttgacttca
     7621 ctattttcta agcttatttc atctgcttct ataatatttg caaacaattt actttctgta
     7681 tactttctaa ttgataatag acctaaactt attaaatttg aagacacttt ttcaaagttg
     7741 atatgctttt tttctagcat acgaaatttt ttgcaataat ctccatgccc cttttttata
     7801 tttatactac ttgctccaac ttcattaaaa ttagaagttc catttaaact aatattaact
     7861 tcttcacaat tcaaaaatcc attacactcc aagattccac ttaaattgat attttcacaa
     7921 tctatattct catttgcttt taaatatcct tctccatgaa taatatccga ttttatactt
     7981 ccttctacat taataattcc actagtatca atttcaacag aattaatatt tcctgtacat
     8041 ctaagaagtc ccgatatctc aacttttgat gccagtatgt ttccttctac tttacttaaa
     8101 ccagatgtaa aaaattcatt acattctgtt gtcccaaaaa cttttgctga accagaagaa
     8161 tcaaatttac tacattttac acttccttct attttgcctg ttcctgatat attaacatta
     8221 tcaaaaattc cacctttaca ttttccaaca ccatcaaatc ttagtgaact taattcttca
     8281 ttcataatga tatcctttct ttaccgtatt ttatctttaa acctaatata acaaaacatt
     8341 gttacgttgt caagtctgat aataatactt aataaagcaa ctatcattta ttttctataa
     8401 ataggcataa aaataatagg gaaatgcttt atttaaacta gaaatctaat ttcttagatt
     8461 gtggtttaaa taaaatatat ccctattact caatctttat atattcattg tataaacttc
     8521 taaaatataa gtctttaaat tctactgtca atacttttta tacactgttt gtgctatcat
     8581 ttcacaccta aaggagtagg ctctccgcac tattctgtaa cctaaaagac actatcttct
     8641 tctatctatt gataaatttt tctattacat cagctagtct ctccatatca gttggataaa
     8701 cttcacctat gctacctatt ctaaatgtat ctatatcagt caatttacct ggatagataa
     8761 caaatccatt atcttttaaa taagtataaa attccataaa ttcaaacttt gcatttttag
     8821 gatataagaa agttgttatt acaggtgatt gagcattatc attcaccaaa gtatcaaatc
     8881 ctaaagattt taatcttgaa gctattgtaa actgattttc tttatatcta gcatatcttt
     8941 tttcaacaga accttcttct tccaattcta ataaagcttg ataaaaagct cttacaacat
     9001 gtgtaggtga agtaaatctc catttaccat tatttttctc catagtctcc cattgcgcat
     9061 acacatcaag agataatgat ttagctatac ctttacattt agataatttc tctttattag
     9121 ctattataaa accaaatcca ggtactcctt gtatacattt gttagaacta ctaactaaaa
     9181 aatctatatt aaaatcttca acatcaatct caattccccc aaaactactc atagcatcaa
     9241 ctatataaat tttgttatat tcttttgcaa gtttccctac ctcttgtata ggatttaatc
     9301 tacctgtagt agtttcacag tgaaccattg atatatgtgt tatatccttg ttttctttta
     9361 ataaattttc tactacattt aaatccactg cttctatatc tttaaatgtg cagtcaacaa
     9421 attctatgtt aagataatta catatatctt tcattctctt tccatatgct ccatttgcaa
     9481 ttacaagaag tttaccatct tttgaaatag tacttcctat tatagcttct actgaaaaag
     9541 tcccacttcc ttgcattaat acacttgtgt atttatctgt attttgggtt gctagagata
     9601 ctaatcttct tctaacatct tgtactaggt tattatattc aacatcccaa gtacaccaat
     9661 cttttaacat agatgctctt actgatttag tagtagatag tggtcctggt gtaagtaata
     9721 tgtaatcatt ttctggtaca aaatctgatt taatagtttc attctctatt tttattagta
     9781 tatcttctaa ctcagccata gtttctatta caaaatgtgc acctgcctct ttaaatttct
     9841 ttgaaactat actcatctta gcttttaatt cttccttatc catgttctct acttcttctt
     9901 gtgtaagccc taactcactt gaacctttta ttacagcaac actccacatt cctgcattta
     9961 cgccttcttt tacatcactt attgtatctc ctacttttac catagaactc ataggagaca
    10021 cccctaaagc ttcagcattt ttatagcaca tccaaggata cggtcttcct tgagatactt
    10081 ctgatggtgt tacaagaaaa tcgggtgagt atcctttctt tgctgcattt ggttcaacta
    10141 tgttcatcat ctctcttgta taacccgttg tagaacctat ttttaatcca ttttttctca
    10201 acttttcaat agtttcaaca acatgaggaa ttggagtcgt atactcttct aatgtctcaa
    10261 ataacattgg ctcaaattca gcataaagtt catttacatc atcttcagtt ggcactttac
    10321 caaacttatc tgaccaaaga ttttttattc tatccatttc acacatttct cttatatggt
    10381 ctatttttaa ttttcccata ggttttcttg cttcttccat tgttacatct atacctcttc
    10441 ttttaaatat ctctataaat acattaagag gtgcaaaaca tccataatct actgttgttc
    10501 cagcccaatc gaatactact gcttttattt tttctccata tatctttttc atatttatag
    10561 cctccataga ttcattttag aatttttgtg tttttaattg tttttacttt taaagtataa
    10621 ctttcatatg ttaattatac atcatattta ggttaaatat atattaaatt tctgttttta
    10681 gttgtattta attgttttta ttttatatca tatttccata aaatcctaag ttacaatcta
    10741 aaatttactt tctaatctta atctatgaac tgactatatt ttatgaattt agttgtatta
    10801 agcatagttg tattatataa aactaagggt ttattttgct tctatccatg ttatatttat
    10861 ctcatttaac ttatccttcc attcattcgg aggttctaca tcacaaataa caaaatctaa
    10921 ttcctctaaa tctgctatct tatacatact agcaattcct attttagaac tatcacataa
    10981 tacaaaactt tcctttgaat tttttatata ttcttttgac aacaatgcct tattttggtc
    11041 tatactacta attccaaaac tatcaagtat accttcacta gatataaaag ctttatctac
    11101 gtataagttt ttcatagtgt ctattgacat tggacatgct gtcctttggt gttttaaatt
    11161 tacttttcca ccaataaata taacttctcc atcaaacaaa ccttgattct cataagaaat
    11221 caattttgat agaactggat aagagtttgt aataattttt aaattgttta cagatattat
    11281 ataatctaca atctgcaaat tggtagtacc ttcatctatg ataattgact cattgtcatt
    11341 tacaaactta gctgcgtatt tagcaatact ttgtttttta tctaggttag tattgtttct
    11401 ctttatatgt tctggttcaa tcactccata atttattttt atagcaccac catatacctt
    11461 ttttaattta ttttcatttt ctaattcttc caaatatctt cttattgttt ctgatgatac
    11521 ttctaatctt tttactaatt ctttagtatt taccttccct tttgaatcta ataatttaag
    11581 tatttctttt tttctatcta tcgaatttaa agacattcat tttcctccta aatataatat
    11641 atttttctcc tataatatta caatgatacc taagatttat gaaatcatat ttatgtatta
    11701 aattttttac ttaattaaag ttattttcaa aagactttgt attatcatta aaaaagtcca
    11761 tcaaatatta gtatcagatg gactttgtaa aattttattt tataaattaa tatttaaagt
    11821 ctaactacta atttattttc ttgtctccaa gaattttact aaattattta cagtagacat
    11881 atcttttctc tctaaatcag ttggttgaag ctctatccct aatctatttt caatttcaag
    11941 taaaacttca attatagcta atgagtctaa aagttccgtc tcaaataaat taaggtctaa
    12001 atcttctctt atctcatcag tccctaatac atcttcaaat atttctataa ctgtttcttg
    12061 cataaatttt cctccttaaa attaagctaa ataacctgag aatatcagca atccaaaaca
    12121 cacaatatgg aatgtaatga aaatctgcac tctctcgaac catttatctt ttttatgttt
    12181 tttataaaat ttagattttc gctgatatat atcagttaac acaagtgcca atccttggta
    12241 tacaccataa gcaacataat accaagttag accatgccaa aatcccattg ttatcatggt
    12301 tatcatctga gccacatgag cagcagtagt ccttttctta aatcttttct ttctcattga
    12361 tgacataaca aatctagaaa aaatataatc tccaaaccat cttgaaagac ttatatgcca
    12421 tcttgtccag aactctttca tatctttact gataaatggc ttgtcaaagt ttataggtac
    12481 ttgtattcca aatatgtacc ctgtacctat agcaaataaa ctatatcctg caaaatcaaa
    12541 gaacaggtat aaactatatg cgtacatata acttaaaata tttataaaac tcatatcttt
    12601 aggtattctt gatacccaat atgtatttat caaaaatgct ataacaaact tataacctac
    12661 tcccatgaca atatttttta ttcctggcag taagtattct tctatatact cttttctaga
    12721 gatgtgtttt tccaagtcct gttcaaatct tcttgaccta tcaataggtc cagaacttaa
    12781 agttggaaaa aataacataa aataaagcat ctttgaaatt ttaacttctt ttatagctcc
    12841 atcataaatt tcaataacca tttgtatagt tctgaaattt aagtatgata ttccaataaa
    12901 tccaattatt ccaaaagaag taactggaga tattttattt attattattg ggagcattga
    12961 tgcaaacaag aatccccaat atatgtattt atttttagtt tttcttctta catattcata
    13021 tcctttaact attattactt caagaataat aaatattacc aaatacttta gctggacatc
    13081 aattcctact attaagaata tcataaacaa acttgccaac ataccataat actttatatt
    13141 cttgcccatt agacctaaaa ttactgctgg tattgatgtt aataataata tataaagata
    13201 aaaataatct ccatactgtg aaaaaatcat taaatttcct ccataagttt ctttctgtct
    13261 attttaccat tgatatttgt tggaaactca gagattattg aaatatttct tggaatcata
    13321 tatgaaggta tatactttcc aagttccttt tttattatca tcccattttt aatattactt
    13381 aaatcattct tttcatttaa ctcaacaata ccttttaagt aagcaatttt ttcgtcctta
    13441 tatactggta aaactactgc attttttaca ttatgtactt ttctcaaatt attttctata
    13501 tcttctattt caattctaaa tccatttaat ttaatctgaa aatcttttct tccacaataa
    13561 tatatatttc catctaaaag atatcccata tctccagttt tataagctct ccactttaca
    13621 ccatctattt catcatagaa aaatacttca tcagtctttt ctttattatt gaagtaacct
    13681 ttagaaactg atggtccaat tattataatt tcaccctttt cattttcttt aagttcattt
    13741 ccatcttcat caagtatttt tattttacaa tttgacatag ggtatccaac tggaagactt
    13801 ttttcatcat ctatagcttt ttgagtcata tcatttacac ttacaccaac agtagcctcg
    13861 gttggtccat atccattgat aattcttgtg tttggaaatc tactcataag ttctttagta
    13921 agattttttg aaagtgcttc tcctatgaat atcattgact caaggttagg aagcatttta
    13981 ctattaaact ctttttcagt tatacatact cctgcaaaag aaggagttga cacccataca
    14041 gctatatctg agatactaaa ttgtctaaat agttctttat agtctgctaa tacatctttt
    14101 gatattgaaa ataatgtagc tccatggata agaccagggt atattgttgt aactgaaagg
    14161 tcaaaagaat atgctggttg gttcattatt actttttcac ttccatctat gtttagatat
    14221 gggcttatcc aatctgaaaa gctatctaaa ttgtttgaac ttatttgaac cccttttggc
    14281 tttcctgtac ttcctgaagt aaatagtata tatgcatttt catcatcttt tacccagttt
    14341 tctttatcta atgattttcc ctgatattca tttattatat catttaattt atccatatca
    14401 attacattga tatctccaaa atttctttca tcactaaaat taaatattac ttttggtttt
    14461 atctctttag tgacttcaaa aactctatca attggaaaac taatatcaag aggcacatac
    14521 gctctacctg attttaaagc tcctatcata catgccatta tcatgttttc tttatttcca
    14581 taaattacta taggagtatc ctcttctttg tatacatcct ttagaaatac tgatatagca
    14641 tctgaatatt catttaaatc tttataagaa agtttatctc cattacacat tagtgctgtt
    14701 ctatctgtat ttgaatattt ttttattcct tcaataattt tcatataaga atgctccttt
    14761 aaaatccgtt ataaataaat ggcaattctt taaatggagt aaatgttaga actcctatcg
    14821 ccaatattat aaccatagtt aaacaaatac tagttaacaa atctttattg ttctttaaat
    14881 attttaaata atctctcaca gacatctacc catcctttcg taccaagatg cattacatcc
    14941 cttaaataat acttatctga ccctttatct ttaaggttta gaacttcaaa gcctttgctt
    15001 tctgctattt tttgtgcttt atcgtagtac tgatttcttt ctttttcaga aataccagtc
    15061 aaattataga atttatccat acttgggatt aatacaacaa ctggattaat acctaagtca
    15121 gtacatacat ttagcatcag tttataattt tcaaactcct tagatgttaa taaatttaca
    15181 tctttgtctc ttcctttgta ttgctctata ccatctttaa aatgctcttt ataatagtac
    15241 ttatcaatat ttagtggatt tttaccaact ctttttttag catcttcaat ggctttcttt
    15301 ctctcatatg accaatttat tggtcttttt ctttttgttg ctctcttttt atctagctta
    15361 attaatcgtt tataaagaat acccttttct ttaagagcta tacagtactt tcttccttga
    15421 aaataaggct ctaaaagcac cttttcagct ttttcaggaa atgtcttagg ttcatatagc
    15481 ttagcatata atgcttctgc tttatactca tcacttcccc ataatagctt acttgatttt
    15541 tttgcatatt caatcttatt ttgtttagat atttttggat tatctaaaaa tcgataaaat
    15601 tgtattggtg aaaatctaga ctgataatgg tgacttgtga ctccgtcttt ttccataaac
    15661 cattgcatag aaattaacaa gacaactttc ttattatcaa tattaggatt catacttcct
    15721 aatattgcag catcttgtaa tgtctgagta taggctctcc caattgcaaa caccttattt
    15781 ttactcctat ttgtattaaa atagtacttt ggatgttgtt ttgttgaatt actaagttct
    15841 gatgacccca aaatcataat atccttttct cttaaaaaat gattatttgc tgtaacccct
    15901 ttatctttta tatcacttaa agtatcatcc ataattggaa gtagattttt ctccctcaaa
    15961 agttcatcag ttttagaatc aagaaatttg tctaacccaa ataaaaatac tacgcctatt
    16021 ataaaaggtg taataaagta tatcaatttt ctcatatttt ctctcctcta aaaatattca
    16081 aatttatgtt attattaatc atattagttt tgatatgtat attcaactac aaaattgtaa
    16141 taaaatcgtc aaaacttttt ttgatactgt tacaaaattg taaaaaaaaa gaaaaaaaca
    16201 ccaaaaataa atttcttttt agcacttttt catacttata atttgacatt ttaacacttg
    16261 gctaagtata caccttacct taacttaaaa ttaaatttat ttaagaatat atattataaa
    16321 ttcttatatt ttctaatata agctatatac tatcagctgg ataaacaata ctactttgtc
    16381 ttcttatctc atccataatt tccattacta ttattgaatt ttcatgagaa tttatatttg
    16441 attctatttt attgttattt ataagactta taaactctac aagttcataa tacattcctt
    16501 ctatttctct aggcttttcc tctgcttttt cttttggaat agatatatct tcttctcttc
    16561 catctctgta tttaataata gctttttcaa atagattaag cttttcaatt attatacttc
    16621 cctcttctcc ttgtatttca gatggtatat aagaatcagc tactttagaa tactgaatta
    16681 ttgcatccat atcatcatat tgaaatacag ctgaaccttc tccatccact ccagtttgaa
    16741 gtatatgaga aactgccttt acagtttttg gagcaccaaa taaattcacc ataggatgta
    16801 tacaatacac tcctatatcc attaaagctc catttgacaa ctctttttta aaggcatttt
    16861 ctataattcc atttttatat ttatcatatc ttgaagaata ctgacaatag cttccaaaaa
    16921 atcttcttat tttccctatt ttatacaagt tttctttaac tgctttaaag tttggaacac
    16981 atgtaattct catagcttcc ataagcaata cattattatc tttggctgtt tttatcattg
    17041 atttgacttc ttttaaatta gatgctaacg acttttcaca aagcacatgt tttttgtgtt
    17101 ttaaacaagt aatggcttgc tttgaatgta atgcatttgg agaagctata tatacagcat
    17161 ctatatcctt cgatttagcc atttgttcta aatcatcaaa aaaaatactt gctccatgta
    17221 gagttccaaa ttctctagct ttttctaaat ttctagaata tacagcaacc aattcaaaat
    17281 ctttaaccct agatgcagct cttagaaata tatttgctat attgcttgtc ccaacaatac
    17341 caaatcttat tttacccatc ataaatcact ccccattttc tacactttat tttaatttta
    17401 ataaaaaact ttacaactta tagatatatt ataaaataat tttatcaaat aaaaaagaaa
    17461 gctaatttta aatgatttca ccattttaac atagctttct tttaactaca atctaaattc
    17521 aacttatatt tttaaagtta tctttctact atatctttat ttcccctcaa aaattttata
    17581 gtaagaattt catccacaaa caatgttact aaaagtatag ttacaagaat tggccaataa
    17641 ggtattataa gactatataa actatcaact atatacactt gaaaatatct aactccaata
    17701 aatgtcaata tagtccaata tacagaaaac tttagacata ctattcctct actattgtac
    17761 ttataatctg aataatccca atagacctta ttaaaatata ctttcataag tattcctgta
    17821 atatattcta ctactgtcgg cactactaga cataataaaa gcaatgcaaa tatattattg
    17881 gggtcaataa atcttgagac ctctattata atcgacattg caattgcata cattggttta
    17941 aatggaccat ttaaaaatcc atcttcttga aaatgccctt gtttataata actatataag
    18001 ttttcaatta tccaacctat aaaaccatac aagaaaaaat taaataaaaa atataataga
    18061 gaacctacat tatccatgaa tattcctcct agctgtttat acttattttt tctcatattc
    18121 atggatttat tctaaatcaa agaaattata tttaatttat ttaattaata ctttatatta
    18181 tagattgtga cattctttag agttcaaact ttttaggtat tctctaactt ccagtaaatt
    18241 ttcaagtatc ttatgtgatt gtgatttaat ttgctcatct ggctctttta aatctggtac
    18301 atttatacat cttatcccac cattgtatgc agcttcaact cccataggcg aatcctctat
    18361 gacaatacaa ttttggggat taacatttat ctttttagct gcttttaaga atatctcagg
    18421 gtttggttta gagtttacaa catcatctcc acaaactata gcatcaaagt aatcttttaa
    18481 atttgcctta gctaatctct ttactgctct ttctctcttt gttgatgttg caacagccat
    18541 tttgtatcca ttttccttta aaaaagagat tagttcattg actcctaatt tgataggagc
    18601 tcccttcctc tccataaatt caatcatatt tttagttttt tcgtcatata aatctattat
    18661 tggtacagaa ctatcgtata tatctgtaag accttctatt attcctttac gatttcttcc
    18721 cataactgat gtataaattt ccttagtcat agtatatcca tacttttcaa acgtctccat
    18781 ccaaaattca agagaaattc tctctgagtc aaacaaaact ccatccatgt caaaaataat
    18841 tccttctact ttttgcatac aattcctcca atattttttg atataattat attacacttt
    18901 ttatgattaa ttttacaacc ctcaatttat ccaattctaa tattgtaaaa ttgtaatttt
    18961 caaaagttac gctttggttt actttaatat ttgaaccaag catataggat atccacccac
    19021 ctatactgtc aaatccatca tgctctatat caagtccaaa atacttatta atatagttga
    19081 tagattcagt tccatcaact aagtaagaac ctgctcctat tttcttaata tgagtctctt
    19141 ctttgtcaaa ttcatcttga atttctccaa ctatctcttc tagtatatct tctattgtta
    19201 ccacaccaga tgtaccacca tattcatcca caactatagc aagttgaagt ttttcctttc
    19261 ttattctctc aagagcttta tctatagtta aattttcaga tatatatata atatctctca
    19321 atatttcttc tagttcaatt ttattttcat ttatcttttg attgtataaa tctcttatat
    19381 gtacaaaacc taatatatca tctttatttt ttctgcatac tggatatctt gtaacacctt
    19441 cctcttttaa aatagctaat atcttttctt cagaatcact ttcatatata cataccatat
    19501 cagttctagg aaccattatc tctctaattt tcttttcttc aaattcaaaa atattatcaa
    19561 ctaatctttg ctctgattca tctataagtc cacttttata actttcttct actaatagtc
    19621 ttatttcgtc acctgtatgg ggttcatcaa cctcatcagc ttgtgaatat ccaaatggtt
    19681 taagaaataa atcagtactt aagttgaaaa aatatattat tggatatgtt aacttataaa
    19741 atcctactaa taaaaaagat gtactcaaca ttattttttc tgtattataa agtgctaaag
    19801 ctttaggaac caactctcct attacaacct ccatcattgt tataattaaa aaagaaatgg
    19861 aaattgatat agtatatata atactttcac taagattaaa tacacttact attggtaaaa
    19921 ttaactcttt aactgtagat tctcccatcc aacctaatgc aagtgaacaa agtgttatac
    19981 caagttgaca tgcagaaaga taggaattta agttgtcttt tattatttta catctttttg
    20041 cattattatt accttcagtt atcatggtat caatcctaga actacgaagt tttaccatag
    20101 caaattcaca agctacaaaa aaagcattta tcaaaacaag cattactata agtgttactt
    20161 ttaataactc ccgcataaag tattggttct tataatgtaa gaaatctata agaactcaaa
    20221 gttcccccct tatatttata aatttaattt ataatatata ttctatcata ttta
//
LOCUS       DAMJWP010000051        19832 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.052, whole genome shotgun sequence.
ACCESSION   DAMJWP010000051 DAMJWP010000000
VERSION     DAMJWP010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19832
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.052"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            147..1652
                     /locus_tag="PC700_001564"
     CDS             147..1652
                     /locus_tag="PC700_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF2580878.1"
                     /translation="MKIQNTEWGYIEWKHTYDENNPKQAMNIYIAVTMPGKKHFNHVH
                     YGQEQMIYILEGEGLYIINGVWKPFYQGMIFYIESGSTHETINTGDREIKELIVSNNV
                     DDVGESEVIDINPNNYLKKTLINYSESTLNLYAAVESIRGQFIDPFKIPLIIYDDSWN
                     IVLKNPYFPLFCFEKCNPMKFPQNCDCMNQKSSNQFVCEYGITIYNIPILYKSNSIGV
                     IRGGYVLLSDLNLDTEHNNLYDIPEGAARSIKRLLKQISKNIINFCSFNDIRKDLQEK
                     EKTIARTYHYGEQLEMNLKVAQDMVTNLRINHHFLFNTLNSMASIALDDGSYDLYSAI
                     IDLSRMFRYTMRSDLRFVELESEILYIKNYLNLQKLRYGDALKVKYLIPEKLYNLSVP
                     FNFIQPIVENAFTHGFRDIDTEKRIEIIARLDSQYAIIEIHNNGTILDGNNIDKIKAG
                     IRSNNGHGLSLIYTKFTSAYGNNFDMDIKSSDNEGTYIMIKIPIENYKEYV"
     gene            1766..2464
                     /locus_tag="PC700_001565"
     CDS             1766..2464
                     /locus_tag="PC700_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDF2580879.1"
                     /translation="MRRALIIDDEEAVLKIITHFIEMKDMPIKIVGKATSGDEAVDKI
                     IGLEPDIVFIDIQMPIYNGLEVIEKTSHLSKKINFVVITAFNYFEYAQKALRLNVKDI
                     LLKPLDMKEFTKSVEKIIGYQYTNNDLLNEILEYINLNYSNDLKLNDCARLFLTNPSN
                     ISRIFKSNLNTTFISYLNHIRIEKSIELLENTTKSINEIAEIVGYNSLNNFYKNFKIE
                     KGMTPKVYKLNTKN"
     gene            2749..3837
                     /locus_tag="PC700_001566"
     CDS             2749..3837
                     /locus_tag="PC700_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580880.1"
                     /translation="MSDKVNMKNIICIAGAYLATAIGSGFATGQEILQFFASQGYMGL
                     IGALISMVLFSFLGAEVITRGRELKLKEPTRIYTFYCGKILGKFYEWFSPIFLFGILI
                     VMISGAGSTLTEYYGINPYLGRVLMAVATLITVSMGLEKLVSILGRIGPVIAVFTIAV
                     GLISLFKHFDGLSMVGEAMKNIEVTKASESPIVSGVIYNTLNIIVMIVFLTGMGASIE
                     SKKDAFWGGLLGGIVFMTAGIVMYLAMISDIGNLYMKEIPSLYLADNISPIVGVCFSV
                     ALILGIYTTAVPLLWSVTNRIVEDEHPKFKLVTTVIAILACIGGFLPFGKLVNILYPY
                     TGYMGLLILVCMVYKAITCRKKSTQSQE"
     gene            4076..5266
                     /locus_tag="PC700_001567"
     CDS             4076..5266
                     /locus_tag="PC700_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2580881.1"
                     /translation="MHELILISEKYKEDILDFFTDIHMHPELSFKEFRTTKAIKDLLV
                     SLDIEILDLGMETGVVGLLKGKYDGPTVALRGDIDALPIYEEVDIEYKSRYDGIMHAC
                     GHDIHTSCLVGCAYVLSHIRDSLHGNVKFIFQPAEEVNKGAKMLVERGVMENPKVDAI
                     FGLHNHPDIPCGKIGVKLGGLMAAVDTIKIEVNGFGGHGGIPNRTIDPIVASSAIIMG
                     IQTIVSRNISPLESAVISIGTINGGTANNVISEKVDMTGTCRSFSNEVRKKISENLEN
                     IVCEIARGYQATAKLDYLFDLPAVINSKEMYTIACKSVCDLYSEDAIVDPIPSTGGED
                     FSIFMEKAPGFFYWLGVGNKEQDCIYQWHNPKFKADKNSILVGTNVLCQSVINYMDKL
                     KNKI"
     gene            5357..6859
                     /locus_tag="PC700_001568"
     CDS             5357..6859
                     /locus_tag="PC700_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HDF2580882.1"
                     /translation="MRALNVVERAGNKLPDPVTIFLLLCIIVVILSAVISNLGVEEIH
                     PSTKEVVKVVNLLEKEQIQSYLGSIVTNFQSFAPLGLVLVTMLGAGVAEKSGFMEVLM
                     KKGISKVPQKLVTVAIVFAGMLSHTAADVGFIILPPLAALVFLGIGRHPLVGMFAAFA
                     GVAGGFAANVMLSTTDVLLAGFTIPAAQMMDPSYQGNPAMNLYFAFISAIVLTFAGAF
                     VTEKFIAPRFTKYNDSTNDKDIQELSELENKGIKYALLSLLVVVVVIVALCIGDNAFM
                     KDPETGSILSSNAPLMKGIVPIITIIFLTPGLVYGKVSKKIKSDKDLVSMMGSSMSDM
                     GGYIVLAFIASQFINLFNLSNLGTILSITGAKLLAESGIPSYGLIIGFILLSGFINLF
                     VGSASAKWAILAPIFVPMFMLLDFNPALTQIAYRIGDASTNPISPLFPYFPVILAFAR
                     RYDKDIGIGTVISNMIPYSVVFTLIEIIILLLFMGIGIPLGPGGGISYVL"
     gene            6931..8187
                     /locus_tag="PC700_001569"
     CDS             6931..8187
                     /locus_tag="PC700_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDF2580883.1"
                     /translation="MLKTTKISDPELYKIVADELVRQEHNIEMIASESTAPTEVLELS
                     GCVFTNKTEEGYPGARFQAGSEEADKLETLAIKRAKEVFGAEHVNVQPYSGSTANYCV
                     YSSILKPNDTVLSMRLDQGGHLTHGSAVNFLHDIYKYEFYGVDPNTGRIDYDALEAKA
                     KECRPKLIIAGASSYPRLIDYERISKVAKEVGAYFMVDMAHVAGLVAAKVIPSPVPYA
                     DFVSSSTTKTFCGPRSGIVLCKAEHAKKLDKGVFPGTLGSIHLNTVAAKAFSLLYLST
                     DKFKKIMEQVVVNAQTLASELISHGFSIVSGGTDNHIVMVDLRSKNLTGKQFEKALEY
                     VGITVNKNVIPDDPQSPFVTSGVRIGLTSISQRGLKEKEVIQIAGIMNKVAENIDNKE
                     VLDECKAEAQELISKFPLYPEGYFED"
     gene            8359..11133
                     /locus_tag="PC700_001570"
     CDS             8359..11133
                     /locus_tag="PC700_001570"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HDF2580884.1"
                     /translation="MRYYNKPTKEVLKYLKTNPEIGLDDNEVEERKLRYGLNEFTIKE
                     GRTFWDELGESLTEPMILILIGAAVISSFVGELHDALGILGAIFIGISIGIITEGKSK
                     KAAQALSKLTENIEVKVLRNGKIIKISKNDLVPGDIVYIETGDMIPADGRLIQSINLK
                     LREDMLTGESDDVAKNADAVLDMEVVYSKTEIIEQDAIPAKQVNMVFGGTLVAYGRGI
                     MVVTHTGDKTEMGKIAQNLSNEDQQTPLQIKLGKLGAKIAGISGVIATLLCMFMIIQM
                     QRKGMLILDTSSILSFLQSLEPAKNAYMVCIALIVATVPEGLPTMINITLAITMQKMA
                     KINALVTKKEACETIGSVSVICSDKTGTLTQNKMMVEVAYVDGKYISGGEYQSNSYFE
                     QNCIVNSTADIEKEDNSFKYIGSATECALLLYHNDKNYNEMRKQTYLISQIPFSSEEK
                     KMSTLIRQEDSDILLSKGAPEVLLKKCSYVQQGKNIVPITPKVEKSILDEIKKLQIKS
                     MRTLGFAYKKMSNSKTEVAMTSEGELNLIGNSRSYMKEDNLVFSGFVGIVDPLREGVK
                     DSIDKAFNAGVDVKMLTGDNINTATAIGNELGLLNDGKKAVEATYIDVLTDKELREEI
                     KGISIVARSKPDTKMRIVSALQKSGEVVAVTGDGINDAPALSQADVGIAMGISGTEVS
                     KNAADIILTDDSFSTIVEGIKWGRGIYENFQRFIQFQLTVNIVAFIIAIISQLTGKDM
                     PFTTIQLLWVNIIMDGPPALALGLEPVRDYVLKRKPINRHSGIIARSMFVNIIINAIL
                     IITIVFTQSAFNILGATSEEQGTVIFSLFAFSALFNALNCREFGLNSTIPNFFKNKLA
                     LQIIVVTGIIQIIFTQVFQSFFNSVSLDFDMWIKIILFASTILLSNEFVKLILRTMKN
                     SRSMNSNSKN"
     gene            complement(11198..11383)
                     /locus_tag="PC700_001571"
     CDS             complement(11198..11383)
                     /locus_tag="PC700_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDF2580885.1"
                     /translation="MFFISDIYTKSPIKFDTPLQKEVYKILQKLDIDFERVDTDEAIT
                     MEDCVQINKKLNMKMVI"
     gene            11755..12933
                     /locus_tag="PC700_001572"
     CDS             11755..12933
                     /locus_tag="PC700_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDF2580886.1"
                     /translation="MFSKILVKTFIRDSENVQNTDVRNKYGYVAGVVGILSNLLLFVI
                     KVFIGMFTSSIAIMADAFNNLSDMASSAITMIGFKLASKPADKEHPFGHGRIEYLSAL
                     IVAFMVMLVGLQFVKSSIERIVNPIPVKFEVIPLILLIVSIMIKIWLSRFNKFVGNKI
                     DSSALKAVSLDALGDVFTSSCVVISFIVARFTNFPIDGYVGIVVSLVILYAGFSLVKD
                     TINPLLGEAPDEEMVNSIIELLLSYKYIIGTHDLIIHNYGVGRCIASIHAEIPSNIDI
                     MEIHEIIDTAEREISEKLDIYLVIHMDPICLEDKEVMSAKKELEEILKKNSLVKSMHD
                     FRIVGKGSKKNLIFDIVVNPSEFSKDMSEDDLKEDITKLVKEINPEYNCVIVVDKDFI
                     "
     regulatory      13059..13144
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            13257..16211
                     /locus_tag="PC700_001573"
     CDS             13257..16211
                     /locus_tag="PC700_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021362860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HDF2580887.1"
                     /translation="MKKGNKKALLISLIMILSMVVSTIYPTVSYASELGENSQIQSGS
                     TNSSTGEEKESDNKKPEQTPEKDKATDNKKPEQTPEEDNSTDNKKPEQTSEEDKSTDN
                     KKPEQTPEGEKPIDNKKPEQTPEGEKSIDNKKPEQIPEEDKSTDNKKSEQALEDEKPL
                     DNKNTEKTPEEDNLLEDENLLKVLEEELNEDYGFVVKINNNTIETESMKKISFNLTYT
                     PTSKGIQAGDSITFKVPDVFNKVNLDYTSECFDKTESNGEYTLTFRELPNGQSVMQGK
                     IGLEAYVKKVDEDTNAKIHIETTGKIESGSGDIDVEIKPGDKTDVPDAKGTLKKLVEG
                     RKSTTVFMPVKNKDINYSIQVNEKQEELKDIILYDELPEGLTLINGSVSVVTSDGKEV
                     SDFNIEQSKNSISVNFGNIDKSYTVKYKARISDKNAKHGNKYKNVARIESDGKKIQED
                     DATVSIFDRGDNYLLTKGHSGATNITQVGQVINYQISINDDKSPISNVVITDNIPEGM
                     RLTTSGEDGHDFRVVEVPMNGSWTPWSKEKIANNISYKVEEKRNESGQVDKVITGFTI
                     NLSKEEVESKFFIAYTLKVISIEDSYINRAVLDANNSEIDKNDEINFKKNSGLISAKK
                     EVDKKVLNSSDNQIVKYKINMSTYGVYDAGQVNLLDEVNPSLKISNIKYSDNLELKKE
                     AGDGKNTIRLVNKYEFKQIKEGEPVQSWVTFDADFTNVKVGETIQNVAQINGSSPPGV
                     ETTKQGYAFEAKKVDALDKNVLSGAKFNLEDAFGNIVVKDLVSDEDGVIQSSVKKPGI
                     YYLVEVMAPSGYEKLKDKIKVEIGNKDIGRIVDIGNIENIRQENPPVNPPVPPNTDEP
                     IVNPPVPPSTDKPRKPSNPSDTEDTIVINPPVPPSEDIINPPIPEILNPPVPPSEEVI
                     ETPVKQIVPIPEVVKPSVSEEKNNKAKDDTLVNPPVPPKTGDSTTIIGEILLVIGAIV
                     GLVVLRRNKNTN"
     regulatory      16397..16482
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            16582..17244
                     /gene="zmp1"
                     /locus_tag="PC700_001574"
     CDS             16582..17244
                     /gene="zmp1"
                     /locus_tag="PC700_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease Zmp1"
                     /protein_id="HDF2580888.1"
                     /translation="MRPSKKLLIAIISIFLISSVPVSAHAYSTTIQQNKDTLSQIVVF
                     PTGNYNKNEANAMVNRLANIDGKYLNVLKQDNLKIKLLSGKLTDEKEYAYLKGVVPKG
                     WEGTGKTWDDVPGLGGSTVALRIGFSNKGKGHDAINLELHETAHAIDHIVLNDISKSA
                     QFKQIFAKEGRSLGNVNYLGVYPEEFFAEAFAYYYLNQDTNSKLKSACPQTYSFLQNL
                     AK"
     gene            complement(17362..18324)
                     /locus_tag="PC700_001575"
     CDS             complement(17362..18324)
                     /locus_tag="PC700_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDF2580889.1"
                     /translation="MKKGYVYIILTTLFFSSMEISLKTVTNDFNPLQITLSRFFVGGL
                     VLLPFAVKRLRALSLSITKSDLKYFAFLGFMCVVVSMSLYQLAVLNTKASVVAVLFSC
                     NPVFVMLLAYLILKEKIYRHNVISLILEVLGIIVIINPLHTKLTLSGIFLTLSSAIIF
                     SMYTVFGKRKTLKFGGIVVTCFSFIFGSLEMLILVLLTKINFIADILNKNKLHIFANI
                     PIFSGYTLHNMPIMIYVFVFVTGVGYALYFMAMEATSTSLTSLVFFFKPVLSPILALL
                     ILKEIIPINMVIGILLIVIGSIISIIPTIITQMHNKHVEALYKE"
     gene            complement(18494..19684)
                     /locus_tag="PC700_001576"
     CDS             complement(18494..19684)
                     /locus_tag="PC700_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2580890.1"
                     /translation="MISNKMQTLVANSSVIRAMFEEGKKLSDIYGEENVFDFSIGNPS
                     VEPPETIKAVINDILNEESPNLVHGYMNNSGYEDVRDAIAEHINKKDGLNLTRENLIM
                     TCGAAGGLNIILKTLLNPGDEVIAFAPYFGEYKNYTENYDGKLIEVPTNIETFEPDLD
                     ALKNAITPKTRALIINTPNNPTGVIYSEELLKNLGELLDSKQKEFNTSIYLISDEPYR
                     EIIYDGAKVPCVLKYYRNSFIGYSYSKSLSLPGERIGYIVANGQMDDFDDVMSSLNVA
                     NRILGFVNAPSLFQRVIARSLDAEVDVNIYKKNFDLLYNSLIDMGYSCVKPNGTFYLF
                     PKAPIEDDKKFCNDAKQFNLLLVPGSSFGCPGHFRVSYCVSYDKVKSSLPAFEKLAKL
                     YNLK"
BASE COUNT         7784 a         2320 c         3380 g         6348 t
ORIGIN      
        1 taataaaaaa aaataattaa tataaaaatg tgtacaaact aatgaatatt gatatagtaa
       61 gctatcatat gaaagagtat gtacacattt tttttactaa tttttcacaa tatgaacata
      121 aataatttac aggaaaggaa tcttttatga aaattcaaaa tactgagtgg ggatatattg
      181 aatggaaaca tacttatgat gaaaataatc caaaacaagc tatgaatata tacattgcag
      241 taactatgcc tggtaagaaa cattttaatc atgttcatta tggtcaagag cagatgatat
      301 atattttaga gggagagggt ttgtatatta taaatggagt ttggaagcca ttttatcaag
      361 gtatgatttt ttatatagaa tctggtagca cacatgaaac tataaataca ggcgatagag
      421 aaataaaaga acttattgtt tcaaataatg tagatgatgt aggagagagt gaagttatag
      481 atattaatcc taataattat ttaaagaaga ccttgataaa ttatagtgaa agtacattaa
      541 atttgtatgc agcagtagaa tcaattcgtg gtcaatttat agacccgttt aaaattccat
      601 taattatata tgatgattca tggaatatag ttttaaaaaa cccttatttt ccactttttt
      661 gttttgaaaa atgtaatcct atgaaatttc ctcaaaattg tgattgtatg aatcaaaaaa
      721 gtagcaatca atttgtatgt gaatatggga taactatata taatatacca atactgtaca
      781 aaagcaatag cataggtgtt ataagaggag ggtatgtact attatctgat ttaaatttag
      841 atacagagca taataattta tatgatattc cagagggagc tgctagaagt ataaaacgcc
      901 tcttgaaaca aatatctaaa aatataataa atttttgctc ttttaatgat ataagaaagg
      961 atttacaaga aaaagagaag actatagcaa gaacatatca ttatggagag caacttgaaa
     1021 tgaatttaaa agtagctcaa gatatggtta ctaatttaag aataaatcat cactttttat
     1081 ttaatacatt aaattcaatg gcaagcatag cactagatga tggtagttat gatttgtata
     1141 gtgcaataat agatttatca agaatgttta gatatacaat gaggtctgat ttaagatttg
     1201 tagaattaga atctgaaata ttatacatta aaaattattt gaatttacag aaattacgtt
     1261 atggagatgc acttaaagta aaatatctta ttccagagaa gttatataac ctttctgtgc
     1321 cttttaactt tattcaacct atcgtagaga atgcatttac acatggattt agagatattg
     1381 acacagaaaa gcgaattgaa ataattgcaa gattagattc tcagtatgct attatagaga
     1441 ttcataataa tggaactatc ttagatggaa ataatataga caaaataaag gcaggtattc
     1501 gtagtaataa tggtcatggt ttgtctttaa tatatacaaa attcacatct gcttatggaa
     1561 ataattttga tatggatata aagtcgagtg ataatgaagg aacttacata atgattaaaa
     1621 ttcctattga aaattataag gagtatgtat agaacttgtg aaaaattaat aagttttata
     1681 aatgttaaga aatatattag atgtttttat gttgttttat atatcttact tttttatatt
     1741 attaagctaa aagggaggta ttctcatgag aagagcacta ataatagatg atgaagaagc
     1801 tgttctaaaa ataataacac attttataga aatgaaagat atgccaataa aaattgttgg
     1861 taaagcaaca tctggtgatg aagcagtaga taaaatcatt ggtttagagc cagacatagt
     1921 ttttatagat attcaaatgc caatatacaa tgggttagaa gtaatagaaa aaacatctca
     1981 tttatctaaa aaaataaact ttgtagtaat aactgcattt aactattttg aatatgctca
     2041 aaaagctttg agattaaatg taaaagatat actccttaaa ccattggata tgaaagaatt
     2101 tactaagtct gtagaaaaaa ttattggata tcaatatacc aataatgatt tattaaatga
     2161 gatactggaa tatataaatc taaactatag caatgactta aagttaaatg attgtgctag
     2221 attattttta accaatccat ctaatatatc taggattttt aaaagtaatt taaatacaac
     2281 ttttatatca tatctaaatc atataagaat agaaaaatct attgaattac ttgaaaatac
     2341 tacaaaaagt ataaatgaga ttgcagaaat agttggatat aatagcctaa ataactttta
     2401 taaaaacttt aagatagaaa aaggaatgac tccaaaagtt tataaactga acactaaaaa
     2461 ttgaaattga taaataaaaa gtcaatatag ataattgttt gagtgtgtct gaataagtat
     2521 actttaatta tgataaaaat taaagcgctt taaacatatt taataatatt aaaccttata
     2581 aaagctgtac ctatttatat aaaaaaatat atataaataa aaagaaaaag atatacaaaa
     2641 taaaaaaagt gaattaaata ctgtttcaaa aagacagaat tttcatttaa ttcactgaga
     2701 gtttatattt cttaaaggaa ggtaacttta taatggaggt aatttaatat gtcagacaaa
     2761 gtgaatatga aaaatattat ctgtattgca ggcgcttatc tagcaacagc aattggttct
     2821 gggtttgcta ctggtcaaga aattctacaa ttttttgcct cacaagggta tatgggttta
     2881 attggagcat taatatcaat ggtattgttt tcatttcttg gtgctgaagt tataactaga
     2941 ggtagagaat taaaacttaa agaaccaaca agaatttaca ctttttattg tgggaagatt
     3001 ttaggaaaat tttacgaatg gtttagtcca atatttttat ttgggattct tatagtaatg
     3061 atttcaggtg caggctcaac tttaactgaa tactatggta taaatcctta cttaggaaga
     3121 gtattgatgg ctgttgctac tttaattaca gtgtctatgg gtttagaaaa attagtaagc
     3181 atacttggac gtattggacc agtgatagca gtatttacaa ttgctgttgg attaataagc
     3241 ttgtttaagc attttgatgg tttatctatg gttggagaag ctatgaaaaa tattgaagtt
     3301 acaaaagcat cagaaagtcc aatcgtttct ggtgtaatat ataatacatt aaatattatt
     3361 gtaatgattg tttttttaac aggaatggga gcttctattg aaagtaaaaa ggatgctttt
     3421 tggggaggtt tattaggtgg aatagtgttt atgactgctg gtatagttat gtatttggct
     3481 atgatttcag atataggaaa tttgtatatg aaagaaatac catcacttta tttagcagat
     3541 aatatatctc ctattgtagg tgtatgtttt tcagttgcat taatattagg tatctataca
     3601 acagctgttc cacttttatg gtctgtaacc aatagaattg ttgaagatga acatcctaaa
     3661 tttaaattgg taacaacagt aatagctatt ttggcttgta taggtggttt tttaccgttt
     3721 ggtaaattag taaacatttt atatccatat acaggttata tggggctttt aattttagtc
     3781 tgtatggtgt ataaagcaat tacatgtaga aaaaaatcaa cacaaagtca ggaatagagt
     3841 cacctattaa tagtttttat ttaaagaaat tttttatata atccgaattt aaaaagtcaa
     3901 aatagttaaa taaattgcaa aacatgataa tatatttaat taaaataata tagtaaaata
     3961 taaatataaa ctaaaattaa atttataaaa aatatcttta taaagattat taacttatat
     4021 aatttgtagt taaattataa aaaataaaaa aataaaattt tggagaggtg ttagtatgca
     4081 tgaacttatt ttaatctcag aaaagtataa agaagatatt ttagattttt ttacagatat
     4141 tcatatgcac cccgaattaa gttttaaaga atttaggact acaaaagcta taaaagattt
     4201 attagtaagt ttagacatag aaatattaga tttaggaatg gaaacaggag ttgttggatt
     4261 attaaaaggg aagtatgatg gtcccacagt agcactgaga ggagatattg atgcattgcc
     4321 aatatatgaa gaggttgata ttgagtataa atccagatat gatggtataa tgcatgcatg
     4381 tgggcatgat atacacacat cttgccttgt aggatgtgca tatgtattgt ctcatattag
     4441 agatagtctt catggaaatg taaagtttat atttcaacca gccgaagaag tcaataaagg
     4501 tgcgaagatg ctagtggaac gaggagtaat ggaaaatcca aaagttgatg ctatatttgg
     4561 acttcataat catccagata taccttgtgg aaaaataggt gttaagttag gtgggttaat
     4621 ggcagcagta gacactatta agatagaagt aaatggattt ggaggtcacg gaggtatacc
     4681 aaacagaaca atagacccta tagtagcatc aagtgcaatt ataatgggta ttcaaaccat
     4741 agtaagcaga aacataagtc cattagaatc agctgttata tcaataggta ctataaatgg
     4801 agggactgca aataatgtaa tttcagaaaa ggtagatatg acaggtacat gtagaagctt
     4861 tagtaatgaa gtacgaaaga aaatatcaga aaatttagaa aatatagttt gtgagatagc
     4921 tcgtggatat caagcaactg caaagctaga ttatttattt gatttaccag ctgttataaa
     4981 tagtaaagag atgtacacaa tagcttgtaa gtctgtgtgt gatttgtatt cagaagatgc
     5041 tatagttgat ccaatacctt ctacaggagg agaagatttt tctatattta tggaaaaggc
     5101 cccaggattt ttttactggt taggggtagg caataaagaa caagattgta tatatcagtg
     5161 gcataatcct aaatttaaag ctgataaaaa ttctatattg gtaggtacaa atgtactatg
     5221 tcagtctgtt ataaattata tggataaatt aaaaaataaa atttaagata gagcattctg
     5281 gagataaagt ttatgagggg gtattagttt gaggaaaatt aattcacagg aaaaaaacaa
     5341 agatagtgga ttattgatga gggcactaaa tgttgtagag agagcaggaa ataaacttcc
     5401 agacccagta accatatttt tattactatg tataattgtt gtaatattgt cagcagttat
     5461 atctaattta ggagtagaag aaatacatcc ttctacaaaa gaagttgtaa aggtagtaaa
     5521 tctactagag aaagagcaga ttcaatcata tttaggtagc atagttacaa acttccagtc
     5581 atttgctcct ttaggattag tactggttac aatgcttgga gcaggtgttg ctgagaaatc
     5641 tggatttatg gaagtgttaa tgaaaaaggg tataagtaaa gttccacaaa aacttgtaac
     5701 tgtagcaatc gtatttgcag gtatgttatc acatacagca gcagatgtag gatttataat
     5761 attaccacca cttgcagcac ttgtgttttt aggaattgga agacatcctc ttgtaggtat
     5821 gtttgcagct tttgcaggtg ttgcaggggg atttgcagca aatgttatgc ttagtactac
     5881 agatgtattg ttagctggat ttacaatacc agcagctcaa atgatggacc catcttacca
     5941 aggcaatcca gcaatgaatc tttattttgc ttttatatca gcgatagttt taacttttgc
     6001 aggagcattt gtaactgaaa aatttatagc tcctcgtttt acaaaatata atgattcaac
     6061 aaatgataag gatatacagg aattgagtga acttgaaaat aagggtataa aatacgcttt
     6121 attatcattg ttagttgtag tagtagtgat agttgcctta tgtataggtg acaatgcatt
     6181 tatgaaagat ccagagactg gttctatact atctagtaat gcacctttaa tgaaaggtat
     6241 agttcctata atcactatta tatttttaac tccaggatta gtgtatggaa aagtaagtaa
     6301 gaagataaaa agtgacaaag atttagtatc tatgatgggt agtagtatga gtgatatggg
     6361 tggatatata gtcctagcat ttatagcatc ccagtttatt aatttattta atcttagtaa
     6421 cttaggaaca attttatcaa taacaggagc aaaattacta gcagagtcgg ggatacctag
     6481 ctatggattg ataataggat ttatattgct atctggattt atcaatttat ttgtaggaag
     6541 tgcttctgca aaatgggcga ttttagcacc tatatttgtt cccatgttta tgctactaga
     6601 ctttaatcca gcattaactc aaatagctta tcgtattgga gatgcttcaa caaatccaat
     6661 aagtccactt tttccgtatt ttccagttat attggccttt gccagaagat atgataaaga
     6721 tataggtata ggtactgtca tatcgaatat gattccatac tcagtggtat ttacattaat
     6781 agaaataata atattgttac tattcatggg tattggcata ccattaggac ctggtggagg
     6841 aatatcttac gttttataga ccattataat aaatttatca atgaagatta aactaataaa
     6901 atatattctt taattaagag gaggattatt atgttaaaaa caacaaaaat ttcagaccca
     6961 gagttataca agattgtagc tgatgagttg gttagacaag aacacaatat tgagatgata
     7021 gcttctgaaa gtacagcacc aacagaagta cttgaattaa gtggatgtgt atttacaaat
     7081 aaaacagaag aaggctatcc aggggctcgt tttcaagcag gttctgaaga agcagataaa
     7141 cttgaaaccc tagcaataaa acgtgcaaaa gaagtatttg gagcagaaca tgtaaacgta
     7201 caaccgtatt caggttctac agctaactat tgtgtatatt catcaatatt aaaaccaaat
     7261 gacactgttt taagtatgcg tcttgaccaa ggtggacact tgacacatgg tagcgcagtt
     7321 aattttttac atgacattta taaatatgaa ttttatgggg tagacccaaa tactggacgt
     7381 atagattatg atgctcttga agcaaaggca aaagaatgta gaccaaaact tataattgca
     7441 ggggcaagtt catatccaag attaatagat tatgaaagaa tatcaaaggt tgcaaaagaa
     7501 gtaggagctt actttatggt agatatggca catgttgcag gattagtcgc agcgaaagta
     7561 atacctagtc ctgtacctta tgcagatttt gtttcttcat ctacgacaaa gactttctgt
     7621 ggaccaagaa gtggtattgt actttgtaaa gcagaacatg caaagaaact cgataaaggt
     7681 gtttttccag gaacattagg gtctatacat cttaatacag ttgcagcaaa agccttttca
     7741 ctattatatt taagtacaga taaatttaaa aagataatgg agcaagttgt agtaaatgct
     7801 cagactctag cttctgaact gatttcacat ggttttagta ttgtaagtgg aggtactgat
     7861 aatcatatag taatggttga cttaagaagt aagaatctta caggaaaaca atttgaaaaa
     7921 gctcttgaat atgtaggaat aacagttaat aaaaatgtta tacctgacga cccacaatca
     7981 ccatttgtaa ctagtggtgt tcgtataggt cttacatcaa tatctcaaag aggtctgaaa
     8041 gaaaaagaag ttatacagat agcaggaata atgaataagg tagcagaaaa tattgataat
     8101 aaagaagtat tagatgaatg taaagcagag gcacaagaac ttatatctaa gttcccatta
     8161 tatccagaag ggtattttga agattaaata ttttgatttg tatagctcac aatttataaa
     8221 gcctgtaaca taaaatttaa taatatataa ccactataag gtgttgatta atcaacacct
     8281 tatttttttt gaaaagtaat atacaagcta tcataagcat ataattcctt atagataatt
     8341 aacaaggggg ataaagttat gaggtattat aataaaccta ccaaagaagt attaaaatac
     8401 ttaaaaacaa atccagaaat tggacttgat gataatgaag tagaagaaag gaagcttaga
     8461 tatggattaa atgaatttac tataaaagaa ggtagaacat tttgggacga gttgggagaa
     8521 agtttgacag aacctatgat attaattctc ataggagcag cagtaataag ttcttttgtg
     8581 ggagaattac atgatgcatt aggtatttta ggggctattt ttattggaat ttctataggc
     8641 ataataactg aaggaaaatc taaaaaagca gctcaggccc tgtcaaaatt aacagaaaat
     8701 atagaagtaa aagtacttag aaatggaaag ataattaaaa tatcaaaaaa tgatttagta
     8761 ccaggagata ttgtatatat agaaactgga gatatgatac cagcagatgg aagacttata
     8821 cagtctataa atttgaaact tagagaagat atgctaacag gagaatctga tgatgtagct
     8881 aagaatgctg atgctgtatt agatatggaa gtagtttatt ctaaaacaga aataatcgaa
     8941 caggatgcaa tacctgcaaa gcaagtcaat atggtatttg gagggactct tgtagcatat
     9001 ggtaggggca taatggttgt cactcatact ggtgataaga cagagatggg taagatagca
     9061 cagaatttat caaatgaaga ccagcaaact ccacttcaaa ttaaattagg gaagttgggt
     9121 gctaaaatag caggaatatc aggcgtaata gctactttat tgtgtatgtt tatgattata
     9181 cagatgcaga gaaaaggtat gttaatttta gatacaagca gtatattatc tttcttacaa
     9241 tcattagaac ctgctaaaaa tgcatatatg gtttgtattg cattaatagt tgcaactgtt
     9301 ccagaaggtt tgccaactat gataaatatt accttagcta taacaatgca aaagatggca
     9361 aagataaatg cattagtaac aaagaaagaa gcctgtgaaa ctataggttc tgtttctgta
     9421 atatgttccg acaaaacagg gactttaaca caaaacaaaa tgatggtaga ggtagcatat
     9481 gttgatggta aatatataag tggaggagag tatcaaagta acagctattt tgaacaaaac
     9541 tgtatagtaa attcaactgc agatatagaa aaagaagata attcatttaa atatatagga
     9601 agtgcaacag agtgtgcatt acttttatat cataatgata agaactataa tgaaatgaga
     9661 aaacaaacgt atttgatatc acaaatacca tttagttcag aagaaaagaa gatgtcaaca
     9721 ttaattcgtc aagaggattc agatatatta ctttcaaaag gtgctccaga agtattatta
     9781 aaaaaatgtt cttatgtaca acaaggaaaa aatattgttc caataactcc aaaagtagaa
     9841 aaaagtattt tagatgagat taagaagtta caaattaaat ctatgagaac attaggattt
     9901 gcttataaaa aaatgagtaa ttctaaaaca gaagttgcaa tgacttcaga aggtgaattg
     9961 aatttaatag gaaattcaag aagttatatg aaggaagata atttagtttt tagtggattt
    10021 gtaggaatag tggacccatt gagagaaggg gtcaaagatt ccattgataa agcatttaat
    10081 gcaggagtag atgtaaaaat gcttacagga gataatataa atacagcaac ggctattgga
    10141 aatgaattag gattattaaa tgatgggaaa aaggcagttg aagcaacata tatagatgta
    10201 ttgacagata aagaactgag agaagaaatt aaaggaatat ctattgttgc aagaagtaaa
    10261 ccagatacaa aaatgagaat agtttcagcg cttcaaaaaa gtggcgaagt tgtagctgta
    10321 acaggtgatg gtataaatga tgccccagct cttagtcaag cagatgttgg aattgcaatg
    10381 ggtatttcag gaacagaagt atctaaaaat gcagcagata taatattgac tgatgatagc
    10441 tttagtacta tagtagaggg aataaaatgg ggaagaggta tatatgagaa ctttcaaaga
    10501 tttatacagt ttcagcttac tgtaaatata gtagcattta taattgctat aatatctcaa
    10561 cttactggta aggatatgcc atttactaca atacaattat tatgggtaaa tataataatg
    10621 gatggtcctc cagccctagc attaggatta gaacctgtta gagattatgt attgaaaaga
    10681 aaaccaataa atagacattc tggaataata gctaggtcta tgtttgtaaa tattattata
    10741 aatgctattc ttataattac aattgtattt actcaatcag cttttaatat attgggagct
    10801 acatcagaag aacaaggtac tgttatattt tctttatttg cgttcagtgc cctttttaat
    10861 gcattaaatt gtagagaatt tggtttaaat agtacaatac caaatttctt taaaaataaa
    10921 ctggcattgc aaataatagt agttacaggg ataattcaaa taatatttac acaagtgttc
    10981 cagagtttct ttaattctgt ttctttggac tttgatatgt ggataaaaat aatactattt
    11041 gcatccacta tattattgtc aaatgaattt gtaaagctaa tacttagaac aatgaaaaat
    11101 agtagaagta tgaactctaa tagtaaaaat taattcttat gaaaccgcaa agtactatat
    11161 attgtaactt tgcggttttt gtttgtttaa tgttgtttta tattaccatt ttcatattta
    11221 attttttatt aatctgaaca caatcttcca ttgtaattgc ttcatctgtg tcaacacgtt
    11281 caaagtcaat atctaacttt tgtaatattt tgtatacctc tttttgcaat ggagtatcaa
    11341 atttaatagg tgattttgtg taaatatcac tgataaaaaa catgatttat ttcattcctt
    11401 tccgtttaaa atcttatagt tatagtgtat cataaggtta taaaaacata aatatagtat
    11461 aaatttaaaa tatgtaaaga caaaacaaaa aaatcatatt ctaagtaata cttgggaaaa
    11521 atgatattat cataataaat aatctttgta aaaacttata tagaatctat taaacagaca
    11581 ctaagccata aaaactttac gaaatataaa atttgtatct aaatagatat atagaataat
    11641 attttactga agtagagtga tttttaagaa ttttatatat aaattatgtt atcataaata
    11701 aagatataga ttataaaatt tgttaataaa ataaagggca gggaggtaag tttcatgttt
    11761 tccaaaatcc tagtcaaaac atttatcaga gatagcgaaa acgttcaaaa tacagatgtt
    11821 agaaataagt atggttatgt agctggtgta gtaggtatat tgtccaattt acttttattt
    11881 gtgataaagg tattcatagg aatgttcaca tctagtatag ctataatggc tgatgcattt
    11941 aataatcttt ctgatatggc atcttcagca ataactatga taggttttaa attagcaagt
    12001 aagccagctg acaaagaaca tccttttggt catggaagaa tagagtattt atcagcatta
    12061 atagtagcat ttatggtgat gttggttgga cttcaatttg taaagtcatc tatagaaagg
    12121 atagtaaatc caattcctgt aaaatttgaa gtaataccac ttatactttt aattgtatct
    12181 ataatgatta aaatctggtt gagtcgtttt aataaatttg tgggaaataa aattgattct
    12241 tcagccttaa aagcagtttc acttgatgct ttaggagatg tatttacttc aagttgtgta
    12301 gttatatcat ttattgtagc tagatttact aattttccta tagatggata tgttggtatc
    12361 gtagtatcat tagtgatact ttatgctggt ttctctcttg taaaagatac tataaatcct
    12421 cttttaggag aagcaccgga tgaagaaatg gtaaattcta ttatagagct attgttatct
    12481 tataaatata taataggtac acatgattta attattcata actatggagt tggtagatgt
    12541 attgcatcta tacatgcaga aattccatcc aacatagata taatggaaat acatgaaatt
    12601 atagatactg cagagagaga aatatctgaa aaattagata tatatctagt aattcacatg
    12661 gacccaatat gtcttgaaga taaagaagtt atgtcagcca aaaaagaact tgaagaaata
    12721 cttaagaaaa atagtcttgt aaaatctatg catgatttta gaatagttgg aaaaggaagc
    12781 aagaagaatt taatatttga tatagtagtt aatccttccg aattttccaa agacatgtca
    12841 gaggatgact taaaagaaga tataacaaaa ttagttaagg agataaatcc tgaatataat
    12901 tgtgtaattg ttgtagataa ggattttatt taattattaa ttaatctcat aaaaggtaaa
    12961 aaatgtaaaa aagatagaca atagtataga ggcataatat aatagaagta tataattgta
    13021 tttttaatat aaataaagca attttaattg tttttatgaa atattataga gatgttgaag
    13081 tatattctat tattgggcac cttatggata tactgagtca gtggtgcaac cggctatgaa
    13141 tataaattta tttattttca tagctttttt tatataattt tttagtaaaa attaatttat
    13201 atacgatatt aatggaagat tataaactat agataaccta gagggggaaa atttatatga
    13261 agaaaggaaa taaaaaggca ttattaatta gtttaattat gattttaagt atggtggtgt
    13321 ctacaatata tccaactgta tcttatgctt cagaattagg agagaatagt cagattcaaa
    13381 gtggttcaac taattcatca actggagagg agaaggaaag cgacaataaa aaaccagaac
    13441 agactccaga gaaagataag gcaacagata ataaaaaacc agaacagacc ccagaggaag
    13501 ataattcaac agataataaa aaaccagaac agacttcaga agaagataag tcaacagata
    13561 ataaaaagcc agagcaaact ccagaaggag aaaagccaat agataataaa aagccagagc
    13621 aaactccaga aggagaaaag tcaatagata ataaaaagcc agagcaaata ccagaagaag
    13681 ataagtcaac agataataaa aagtcagaac aagctttaga agatgaaaaa ccactagaca
    13741 ataaaaatac agaaaaaact ccagaggaag ataatctatt agaagatgaa aatttattga
    13801 aagttttaga agaagaactt aatgaagact atggatttgt tgtaaaaatt aataacaata
    13861 caatagaaac ggaatcaatg aaaaaaatat catttaattt aacttatact ccaacatcaa
    13921 aaggaataca agcaggagat tcaataactt ttaaggtgcc agatgtattc aataaagtta
    13981 atttagatta tacaagtgaa tgctttgata aaacagaaag taatggagag tatacactta
    14041 catttagaga attacctaat ggccaaagtg taatgcaagg taaaataggt ttggaagcat
    14101 atgttaaaaa ggttgatgaa gatactaatg caaaaataca tattgaaacc actggaaaaa
    14161 tagaaagtgg aagtggagat atagatgtag aaataaaacc tggagataaa acggatgttc
    14221 cagatgctaa aggaacactt aaaaagcttg ttgaaggaag aaaatcaact actgtattta
    14281 tgccagttaa aaataaagat ataaattaca gtatacaagt aaatgaaaag caggaagagt
    14341 taaaagatat tattttgtat gatgagcttc ctgaaggatt gacattgata aatggttctg
    14401 taagtgtagt aacaagtgat ggaaaagaag ttagtgattt taatattgaa cagagtaaaa
    14461 attctatttc agttaatttt ggaaatatag ataaatcgta tactgttaag tataaggcta
    14521 gaatcagtga caaaaatgca aaacatggta ataaatataa gaatgtagct aggattgaat
    14581 ctgatggtaa gaagatacaa gaagatgatg ctactgtaag tatatttgat agaggagata
    14641 attatcttct tactaaagga cattcaggag caacaaatat tactcaagtt ggtcaagtaa
    14701 taaactatca gatttcaata aatgatgata aatcaccaat aagtaatgtt gttataacag
    14761 ataatatacc agaaggtatg agattgacta cttcaggaga ggatgggcat gattttagag
    14821 ttgtagaagt tccaatgaat gggagctgga ctccttggag taaagaaaaa attgccaata
    14881 atatttcata taaagtagaa gaaaaaagaa atgaaagtgg gcaagtagat aaagttataa
    14941 ctggatttac aattaatctc agtaaagaag aagtagaatc aaagtttttt atagcttata
    15001 cattaaaggt aattagtatt gaagatagtt atataaatag agctgtcctt gatgcaaata
    15061 atagtgaaat tgataaaaat gatgagataa actttaagaa aaattctgga ttaataagcg
    15121 caaaaaaaga agttgataaa aaggttttaa atagtagtga taaccagatt gttaaataca
    15181 aaatcaatat gtcaacttat ggggtttatg atgcaggaca agtaaatctt ttggatgaag
    15241 taaacccatc tctaaaaata tctaatataa aatattctga taatcttgaa cttaaaaaag
    15301 aagctggaga tggaaaaaat acaataagat tagtaaataa atacgaattt aaacagataa
    15361 aagaagggga acctgtgcaa agctgggtta catttgatgc agacttcaca aatgtaaaag
    15421 ttggggagac tatacaaaat gtggctcaga ttaatggttc aagtcctccg ggagtagaga
    15481 ctactaagca aggttatgct tttgaagcta aaaaagtgga tgctttagat aaaaatgtac
    15541 tttcaggtgc taagttcaat ttagaagatg catttggaaa tatagtagtt aaggatttag
    15601 ttagtgatga agatggtgta attcaatcta gtgtaaaaaa accaggaata tattatttag
    15661 tagaagtgat ggctccaagt ggatatgaaa aactgaagga taaaattaag gttgaaattg
    15721 gtaataaaga tattggtaga atagtagata tcggaaatat agaaaacata agacaagaaa
    15781 atccaccagt aaatccacct gtaccgccaa atacagatga accaatagta aatccaccag
    15841 tgccaccaag cacagataaa ccaaggaaac catcaaatcc atctgataca gaggatacta
    15901 tagttataaa tccaccagta ccaccgtcag aagatataat aaatcctcca ataccagaga
    15961 tattgaatcc accagtacca ccatcagaag aggtgataga aactcctgtt aaacagattg
    16021 ttccaatacc agaagtggta aagccatcag tgtcagaaga aaaaaataat aaagctaaag
    16081 atgatacttt ggtaaatcca ccagtaccac caaaaacagg ggatagtact acaattattg
    16141 gagaaatact tttagttata ggagctattg taggacttgt cgtattaaga agaaataaaa
    16201 atacaaatta gaccctaaca ttataaaaaa tataagttat attaaaaaat atcttctaaa
    16261 acaatattta ataatgaaaa tattgttaga agatattttt ttttgaagat ttatgtaaaa
    16321 aaataataac tttttagaaa aaagttccaa agggatatag acaattaaaa agatataagc
    16381 tataatatag atataaaatt aaatgaggca aaactaggga aacctagtca cgcaaagcta
    16441 taggggctaa agtttagtaa accatgccag ccagttgccg aaaagagtaa aaatttcttt
    16501 ttgtatatac aaagatactc ttgggtatct tttttatttt gaaatttatt actcccggcc
    16561 taactcaaag gagggaataa tatgagacca agtaaaaaat tattaatagc tataatatca
    16621 atattcttaa taagttcagt tccagtaagt gcacatgcat atagtactac tatacaacaa
    16681 aataaagaca cacttagtca aatagtagtt tttccaactg gaaattacaa taaaaacgaa
    16741 gctaatgcta tggtaaatag attagctaat atagatggaa agtatttaaa tgtattaaag
    16801 caagataact taaaaataaa attattaagt ggaaagttaa cagatgaaaa agaatatgct
    16861 tatttaaaag gtgtagttcc taagggatgg gaaggtactg gaaaaacttg ggatgatgtt
    16921 ccaggcttag gtggaagtac agtagctctt agaataggat ttagtaacaa aggtaaaggg
    16981 catgatgcaa taaatttaga attacatgaa acagcacatg caatagacca tatagtatta
    17041 aatgatatat ctaaatcagc acaattcaaa caaatatttg ctaaagaagg tcgttcttta
    17101 ggtaatgtaa actacttagg tgtatatcca gaagaatttt ttgcagaagc atttgcatat
    17161 tactatttaa atcaagatac aaatagtaag ttaaaatcag cttgtccaca aacttatagc
    17221 tttttgcaaa acctagctaa atagagcttt aataaattta aaataaatat aaaagagatg
    17281 ctattataat agcatctctt ttatattgcg tttaaatcta ataaattttg tcttatgtat
    17341 aacttgatag attatcaatt actactcctt atacaaggct tcaacgtgtt tattatgcat
    17401 ttgagtaatt atcgtaggta taatagaaat tattgaacca attactatca ataaaattcc
    17461 tataaccatg tttataggga taatttcttt aagtattaat aatgctagta ttggagatag
    17521 tactggttta aagaagaata ctaaagatgt aagactagta gatgtagctt ccattgccat
    17581 aaagtaaagt gcataaccaa cacctgtaac aaatacgaat acataaatca taatgggcat
    17641 attgtgtaag gtatatccac taaaaatagg gatatttgca aatatatgaa gtttattttt
    17701 atttaatata tctgcgataa aattaatttt agtgagtaaa accaatatca acatttccaa
    17761 actaccaaat ataaagctaa agcaagttac aactattcca ccaaatttta aggtttttct
    17821 tttaccaaat acagtataca ttgaaaaaat aatagcagaa gataatgtta agaatattcc
    17881 actaagagtt agttttgtat gcaatggatt tattataact ataattccaa gaacttctaa
    17941 tataagtgaa attacattgt gcctatatat tttttccttc aaaattaagt aagctaaaag
    18001 cattacaaag actggattac aactgaacaa aacagcaact acagatgcct ttgtgtttag
    18061 aacagccagt tgatacaatg acatacttac tactacacac ataaaaccta aaaatgcaaa
    18121 gtattttaag tctgattttg taatagaaag actaagagct cttaatcttt ttacagcaaa
    18181 aggtagtaag actaaaccgc caacaaaaaa tcttgataaa gttatttgta agggattaaa
    18241 gtcattagtg acagttttta gactaatttc cattgaacta aaaaataagg tagtcagtat
    18301 tatataaaca taaccttttt tcaagtcaaa cacctccttg taatttatat tagtactata
    18361 gataagaata ttcaagcata ttggaataat atggtatgta taaaatatag tacataaaat
    18421 tataaactac cttaatatag aatgtaagta ttctatacta aggtagttaa ttatttgaat
    18481 tactttgatt tagttatttt aaattatata atttggctaa tttttcaaat gctggtaaag
    18541 aacttttaac tttatcataa gatacacaat atgatactct aaaatgacct ggacaaccaa
    18601 aagaagaacc tggtactaat aataaattaa attgttttgc atcattgcag aattttttat
    18661 catcttcaat tggagctttt gggaacaaat agaatgtacc attaggttta acacaagaat
    18721 atcccatatc gattaaacta ttatatagaa ggtcaaaatt ctttttataa atatttacat
    18781 caacttctgc atctaaactt cttgcaataa ccctttgaaa aagagaagga gcatttacaa
    18841 atccaagtat tctatttgca acatttaatg aagacataac atcatcaaaa tcatccattt
    18901 gcccattagc aacaatatat cctatacgtt caccaggtag agataatgat ttactataag
    18961 agtaccctat aaatgaattt ctatagtatt ttagtacaca aggaactttg gcaccatcat
    19021 aaattatttc tctatatggc tcatcagaaa ttagatatat gcttgtatta aattcttttt
    19081 gttttgaatc caataattct ccaagatttt ttaataattc ttcagaatat atgacaccag
    19141 taggattatt tggagtgttt attattaaag ctcttgtttt tggagtaata gcatttttta
    19201 gagcatctaa atctggttca aaagtctcta tatttgtagg aacttctatt aattttccat
    19261 catagttttc tgtataattt ttatactcac caaaatacgg tgcaaaagca ataacttcat
    19321 caccaggatt taaaagagtt tttaaaataa tatttagacc accagcagca ccacaagtca
    19381 taattaaatt ttctcttgta agatttaatc catctttttt atttatatgt tcagcaatag
    19441 catctcttac atcctcatat cctgagttgt tcatatatcc atgaactaaa tttggagatt
    19501 cttcatttaa aatatcattt ataactgctt taatagtctc tggaggttca acacttggat
    19561 ttcctatact aaaatcaaaa acattttcct caccataaat atcagaaagt ttctttcctt
    19621 cttcaaacat agctcttata acagaactat ttgcaactaa agtttgcatt ttattagata
    19681 tcatatgtaa cactcctttt tatagattta tattgcttat gtatatatta ccatattttt
    19741 actgaattta gacaattttc gaaaaattac ttgtttgaaa atataaactt ttatataaaa
    19801 tatatttatg tacagtgcat attaaaaatt cc
//
LOCUS       DAMJWP010000052        19769 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.053, whole genome shotgun sequence.
ACCESSION   DAMJWP010000052 DAMJWP010000000
VERSION     DAMJWP010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19769
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.053"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            52..267
                     /locus_tag="PC700_001577"
     CDS             52..267
                     /locus_tag="PC700_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3791 domain-containing protein"
                     /protein_id="HDF2580891.1"
                     /translation="MDERKLEFSIFCIEALAEKLNIDSEGVYKLLKHDTDILDNYIIP
                     CYEALHSQSKSYIVEDLIQVLEEKGVM"
     gene            267..746
                     /locus_tag="PC700_001578"
     CDS             267..746
                     /locus_tag="PC700_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
                     /protein_id="HDF2580892.1"
                     /translation="MIVYHDSYCVAKTPNIIFLRDSLDFGKGFYVTSIKTQAINWIER
                     FKKRGKKAYLNSYSLDIDKLKENCKVKIFNSYNLEWLDFILECRNMSDVYMKYDVIIG
                     GIADDKIYNTIELYQDNLIEKDEALKRLKYYKPNEQICITNQNVIDKYLKFRCEEEV"
     gene            752..982
                     /locus_tag="PC700_001579"
     CDS             752..982
                     /locus_tag="PC700_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580893.1"
                     /translation="MEANKTLLQILYKKIILEFSNQTGKSLEESMDYLYTSETYKLIS
                     EGVSDMHCKGHIYLAQELMLENGLMYHKGYPR"
     gene            complement(1098..2264)
                     /locus_tag="PC700_001580"
     CDS             complement(1098..2264)
                     /locus_tag="PC700_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDF2580894.1"
                     /translation="MATKRANGEGSIGKYKDGWRSRIMIGYNEDGKPIRKEFYGKTQK
                     EVKDKLDAYKKQYYLSSDISDDKITLEHWFYNWLFEYRIKDLKPKSFERYESIYRNYI
                     KDTDLGNVKLKDLRVSHMQKYYNRLLDSNKSIPTIRQINTKLKTCLSEAEKQGLIQRN
                     YCTMVNIPVEKKENRLEILTLEQQKNFIKAIEGHKLEVLFLVALGTGLRLGELLGLKW
                     FDIDFKNSNLTVKRTLQRTYFIDKNGNRELKVLEQEPKTSNSYRTVPIPKDVLNKLKE
                     HKVNQNVDILKAGELYRNDNYVFCNELGVPIDDKRPLRNLKSILSSLDIEPIKFHGLR
                     KTYATRLFENDVPPKTVQVLMGHYDISITLNIYTQVMEDKKVEAVEKLDKIFSL"
     gene            complement(2254..3006)
                     /locus_tag="PC700_001581"
     CDS             complement(2254..3006)
                     /locus_tag="PC700_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDF2580895.1"
                     /translation="MNKSYIPKYTHKFPTNLPHGDKKYTLTYIRRMISEFVYDMGNLE
                     NNPFTFPEVQTLLDGITVGGHKLSDQNQILNIKSSWDYIIKLSNKENLSLNKDTICSI
                     HKIVAKDEALIVGDFRNGNIGIAGTTQYECIEATLLNNLFISDISIINKITNPLEKAI
                     IINLWLSYCQFFYDGNKRTARLTSNLILISNDIGVLSIPAKHKQEYNTLMLNFYETLE
                     ADEVIKFLLEKCITFFDGYNYKSYKELFKNGN"
     gene            complement(3098..3589)
                     /locus_tag="PC700_001582"
     CDS             complement(3098..3589)
                     /locus_tag="PC700_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2580896.1"
                     /translation="MINNTIGEKIKQYRKSINMSRNELANKIGCSVHAIAKYEQNQRI
                     PSLDMIRKISTALSIAINKLIVEENDLDTLTNNNINLKVFDKELDLVVHILENYGYDI
                     KTDYKKKFEITDNADTIKPISLSFSDFKDFSKNLNWVINGLVENFIQENSNGLIEDED
                     EEY"
     gene            complement(3840..4604)
                     /locus_tag="PC700_001583"
     CDS             complement(3840..4604)
                     /locus_tag="PC700_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2580897.1"
                     /translation="MQIGKNLKKIRKQKELTQIQLAEISGISRNALINYENDKRIPSI
                     DTLSKLAKALKIEKTIFYFDIENEVMTNEDVLYFLQSLFPEEYYSFDEFLNAISNLME
                     DDDIRITSSLLLGAWSDKDQYYKLGEKLELNENQVYNWILSDCVSNTELDFSLHPDDI
                     KEIIKNDVLIKQSIDELLDIGLSEENIEAIYDYFSEATDNGSNIRLKKLISQKENMTI
                     KNNDYFKLLQENNELKEKNKKLENTLKEIKKLLTKI"
     gene            4751..4951
                     /locus_tag="PC700_001584"
     CDS             4751..4951
                     /locus_tag="PC700_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2580898.1"
                     /translation="MGINLKIFRIKKGLKQQELADRVGISRYYLSNLETGKANNPSND
                     LMIKLSRALDTTVEELFFSKEE"
     gene            4955..5269
                     /locus_tag="PC700_001585"
     CDS             4955..5269
                     /locus_tag="PC700_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580899.1"
                     /translation="MKENNNLEVLKTLQATLNIIVKNEEEAQEKKQGYKIIENAKRNK
                     AELITCKEASKLYPIGESKFRQLCHSKNKGFPCIWINNRVYIIKNKLDDWFIENANGI
                     KL"
     gene            5291..6034
                     /locus_tag="PC700_001586"
     CDS             5291..6034
                     /locus_tag="PC700_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580900.1"
                     /translation="MSNKGWIKLYRELLDKSIWKDIKPERKVILITLLLMASHKENHV
                     FIDNKRIKIKSGQFISSYSKIAERCGKGVSYQMVRSCLLVFKNAGFLTYETTNKYIVI
                     TIENWELYQENIENQQPNQQTINNQSTTIKNIKNENNIIYSEIISKFNSTCIELSKVK
                     KLTDTRKRRINSIIREYDKEAVLEVINIVSKTSFLNGNNNTGWKANFDWIMNSSNFTK
                     ILEGNYHSKEIVINKETGIYNPDDSQLKW"
     gene            6047..7381
                     /locus_tag="PC700_001587"
     CDS             6047..7381
                     /locus_tag="PC700_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2580901.1"
                     /translation="MNNSNNLIKNTEAEQSILGSILLDSTIVEELEQMLNEDMFYHEE
                     HKYIFNAIKEINKRNEVIDIITLTEELKRKGHLNNIGGISYITSLSTIVPTVSNFTSY
                     VSIVIEKYKNRNIIDKFNKFNLGEIDDNTLIYQLENDINKANQKLIKKDSSITAISER
                     AFEYINDEMNEGIKTGIKFLDDTIGGLYGGELTTIAAKSGRGKTALALQILRNVIFQG
                     KKVLFISGEMSDIQILFRNIASLTGISVVKMKNRNLNDDEIVKYIQALSIINQENNLY
                     INDSVNTVSGIKREIKSTKPSLVIIDYLQILDYEGKEVGEAKFADLSRQIKKITLDFN
                     IPVIQLAQLNDELKDERPKGDRVMRSSKQIYMDSNSVIYIHRPTEKEALNYCKTMGIE
                     DIRELRRREKGFGANLSEIIVAKNRDGESGFRPYWYIGNLFSFEECKKIQEI"
     gene            7631..7903
                     /locus_tag="PC700_001588"
     CDS             7631..7903
                     /locus_tag="PC700_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580902.1"
                     /translation="MYDEIIEIYFDYWINEHMYRSILEKDEYYIECWKEVKKINELLE
                     INLEDDKKYLLVELNDLFNSLIEFERELSYKLGFKTGAKFIIELLK"
     gene            8176..9303
                     /locus_tag="PC700_001589"
     CDS             8176..9303
                     /locus_tag="PC700_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDF2580903.1"
                     /translation="MIIPKLEPNDRKREFNKYSSEQINKVVYNYLFKNNMSNRKIDDE
                     ILDLSSKQSKGYQSMGIVHHLGINKNHKGIFTGYTIDKAIEVLKEDEIHFKQIINILL
                     EIRNINEYPIIVDSWELLDEKIALKTIDKSTLKHNGTGVPIEIRYFFEINNLGKNEKK
                     NVNFKYNDNYYSAVIEMDKLENPRSRIIWKSKFRDLLRSIFSEYYEKFLENKIEGKTN
                     LPKIRFIKLDSNNFNIEFIFTAEIEDDVLEEISQDNPLNINTINIELETEIRKEGKVK
                     YVYGKQYERNPRNRIEAIKYHGTKCIVCGFDFEKTYGDRGKGYIEIHHIKPLSSVGEE
                     SNINPKTDLVPICSNCHRMIHRKKDNVLSIDDLKQIIKDSK"
     gene            9783..10430
                     /gene="cas5"
                     /locus_tag="PC700_001590"
     CDS             9783..10430
                     /gene="cas5"
                     /locus_tag="PC700_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HDF2580904.1"
                     /translation="MILNKIEIIGDMAHFKIPMHSKIQRTYEIPPISTVVGILKNIYG
                     EEIDDFILGYVIYHDGTFKDLQTIYKEINPNVKTLTDSDRFQTDVCPIEYLINPKLII
                     YTNIDKNIEFNEPLCLGKTNCLAKVLSLSEIEVNLIDKKAIGYNQYTDINIGDGMIKR
                     INTLTEYNEKKGYYDIYSSMVRENDEFEYGKYYDEDEQQNIFLWKWKKGGVIKCI"
     gene            10421..11185
                     /gene="cas6"
                     /locus_tag="PC700_001591"
     CDS             10421..11185
                     /gene="cas6"
                     /locus_tag="PC700_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HDF2580905.1"
                     /translation="MHLVRRHLILTVDNEVVLDYNYQYELMKRIYEAIEINDKRKALS
                     LHNEGYKVDKKVFKLFNYTIMFENAKYLKEGIHLNPQTKIKLILSGYDDILNNIIKGF
                     IKCKVFKLNNIEFKVSDIEEDSKKNFNNITLYKVRSPIVASLYDLKSRKQVYLNPMQE
                     EFYKALHDNLGNKYKLIHNKEYTGELYFDIEDVLAVKKKYITNIKGKGFIIGYTDFEI
                     FVQANKDMQEVIYYCGLGEKNSIGMGLLTYITSRRA"
     gene            11187..12353
                     /locus_tag="PC700_001592"
     CDS             11187..12353
                     /locus_tag="PC700_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580906.1"
                     /translation="MDRSYNVFVDNGLYVLAYYLNKDINDITYQDIENSIDLMSDKIE
                     EFVSCEKYSNLKSMCFSNSTLTQPKGKVTLNEKLKGFVKNQGDEYCSLCGQYKAKVKI
                     EDKEYNIGRSYMPNLVANTFYNFSNNLQGLNVCPYCLVLTMYSILNCRVSRYAFLYNS
                     TSSEFMEDYTFHIQKENLTDVELGAKKEKEKYSIVKLLESLVCKYNLFNGDIEQYMFN
                     NSGQSQDIQVNRIKNKYVNLLIKLQEKALLSNFEKLHLDRYILNGTLESNYLREVYRV
                     KKEEKMDEKEQKNFDELVIFLNREVNVLKKVKIDIIKKLTDKLKTSDLDIDKIYKELK
                     MLGKFYAYEDWLVNLNEMYYEKTGEKLFEAEDYIELDDIRSFRQIKNLILVSLI"
     gene            12374..13444
                     /locus_tag="PC700_001593"
     CDS             12374..13444
                     /locus_tag="PC700_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HDF2580907.1"
                     /translation="MENKNSNRMLNIGYAIEVLCGRLNGSSSEVEKKNNSYKNSTYTK
                     KIGSRSRISSQCQKYNQKLFMEQVCGYEQNVKTKDGKQVKLVPNPFKYATDDIFGFMM
                     AEKLSIEEDVYKELSDEEKEIYKKNKKKYEVNITKKRKSRWQMNSLINVSNRKVEWEW
                     NVCGTTGDSMPYQVEVYSGVHTNIANLNIGKIGEYDISDIASEHRDYTTLEAKSIGVE
                     KLDKEEKFKRIEALLKSLEYLSVEGNKTNHLTDTKPKFVILGEYSWGNNVFQGIIKEN
                     GLDVEMLKESIEQNEEFRKSDIWIGINKFYDDTFNEIEVNNEKEQIKKYLEEEFEEYD
                     FMKISNVHQAFESYLKYLKETL"
     gene            13455..15440
                     /locus_tag="PC700_001594"
     CDS             13455..15440
                     /locus_tag="PC700_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HDF2580908.1"
                     /translation="MNNLYEYKAKSDGTSIDKHNEDMNYIMNQLVSIYNISNDMKNDL
                     YEAIVCHDVGKVVKSFQDNIESSTRRVRHELLSASIKYLNRGQKLAILTHHKDIKFLE
                     GQITNSIYEIELKELKELLDIEVEDIRPFIKEISRARNDDTRKLENILLKGYLQYCDH
                     IASAGIKSIDKGFNSIKDFSIPKGVSYNSIQEKVLNMDKNEDIMIMAMTGLGKTATSL
                     YWSNLVQNEYKSKRVYYILPYTASINSLYENLYNRNISVSMLHSKAPYFLNKISDEED
                     IKQLYQIYKKSVKQINVCTIFQLVKSVFSCKRFEMLLSQLKDSIFIVDEIHCFDTRVL
                     CYTLEFLKYLKENLNVKICIMSASIPTCLKKLIQERLQISTIIEPDSKDLIFRHRLNR
                     LSEGILDSIHKIENDLMQGKRVLICVNSVDLSQELYGRLQKYNPKLIHGRFNTRDREQ
                     AEKGIEESFLLIGTQSIEVSLDISYDVLYTELAPLDSLLQRFGRVNRRGDNAELSNIY
                     IYDYDRKLNIYDEDIINRTDIVISDIIHNDNSIVDESKVPSYLDNVYLDIDMDKYNKH
                     KVALEIIMKNMRLGVWNKSANNDMLNNDTISVVPECLLEEYKLYIDKKMYLEASSLFV
                     NVSVKRYNYNKKMFYECGDIVVTSYSYNDKLGLIFDC"
     repeat_region   15693..16315
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=15693..15721
                     /rpt_unit_seq="gtttaaattacactaagttagttataaat"
     gene            16760..18316
                     /locus_tag="PC700_001595"
                     /pseudo
     CDS             16760..18316
                     /locus_tag="PC700_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003444896.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            19494..>19769
                     /locus_tag="PC700_001596"
     CDS             19494..>19769
                     /locus_tag="PC700_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580909.1"
                     /translation="MGEKMQSVICKIFDNKLLISNDLSEQDDVLYEVMDKFIDDIEYI
                     SKLGDEQIKSMLMEVNKFEEDSTEFELYMKNLCYLKQSYFIDTNDKSE"
BASE COUNT         8054 a         1958 c         3076 g         6681 t
ORIGIN      
        1 aattaataaa aatatatata ttatataagt ggataggagt tgaggtttgt aatggatgaa
       61 agaaaactag aattttctat tttttgcatt gaagctttag cagaaaagtt gaatatagat
      121 tcagaaggag tttataaact tcttaaacat gatactgata ttttagataa ctatataata
      181 ccttgctatg aagcactaca ttctcaaagt aaaagttata tagtggaaga tttaatacaa
      241 gtgcttgaag aaaagggcgt gatgtaataa ttgtatatca tgattcatac tgtgtggcaa
      301 aaacgcctaa tattatcttc ttaagagatt ctttagattt tggaaaagga ttctatgtaa
      361 ccagtataaa aacacaggca attaactgga tagaacgttt taagaaaagg ggtaaaaaag
      421 catatttaaa tagctattct ctagatattg ataaattaaa agaaaactgc aaggtaaaaa
      481 tcttcaatag ttataattta gaatggcttg actttatatt agaatgtaga aatatgagtg
      541 atgtatatat gaaatatgat gttataatag gtggaattgc tgatgataag atttacaata
      601 caatagaact gtatcaagat aatcttattg aaaaagatga agctttaaaa agattaaaat
      661 actataagcc aaatgaacaa atatgtataa ctaatcaaaa tgtaatagat aaatatttaa
      721 agtttagatg tgaagaggag gtatagtgct tatggaagca aataaaacat tattacagat
      781 actatataaa aaaattatat tagaattttc taatcagaca ggcaagagtt tggaagaaag
      841 tatggattat ttatatactt ctgaaacata taaacttata agtgagggtg tatcagatat
      901 gcactgtaag ggtcatatat acttggctca agaattaatg ctagaaaatg gattaatgta
      961 tcataaaggc tatccaagat aaatagttaa aactagatac gaattaagga tacaagataa
     1021 tataaaatta atttgtatcc ttatttgggt tttgtaatta actatatgaa atcatagtat
     1081 ttttatgtat ttgattttta caatgaaaat attttatcca acttttctac agcttcaacc
     1141 ttcttatctt ccataacttg agtatatata tttaaagtta ttgatatatc ataatgaccc
     1201 attaaaactt gtacagtttt aggaggaaca tcattctcaa aaagtctagt tgcataagtt
     1261 tttctaagac catgaaactt tataggttct atatctaagc tgcttaatat agattttaaa
     1321 ttccttaaag gtcttttatc atctataggg acacctaatt cattgcaaaa tacataatta
     1381 tcatttctat ataattctcc agcctttaat atatccacat tttgatttac tttatgctct
     1441 ttcaatttat ttaaaacatc ttttggaata ggtacagtcc tgtaagaatt tgatgtcttt
     1501 ggttcttgct ctaatacttt aagctctcta tttccatttt tatctataaa atatgttctt
     1561 tgaagggttc ttttaactgt taaattagaa tttttaaaat caatatcaaa ccatttaagt
     1621 cctaataatt ctcctaatct aagtccagta ccaagagcaa ccaaaaataa tacttctaat
     1681 ttatgcccct ctatagcttt tataaaattc ttttgttgtt caagagtcaa tatttcaagc
     1741 ctattttcct ttttctcaac tggtatattt accatagtac aataattacg ttgtataaga
     1801 ccttgttttt cagcttctga taagcaggtt ttcagtttag tgtttatttg ccttatagta
     1861 ggtatacttt tattggaatc aagcaatcta ttgtaatatt tttgcatgtg agatactctt
     1921 aagtccttta acttaacatt gcctaaatca gtatctttaa tataattacg atagatgctt
     1981 tcatatcttt caaatgactt tggttttaaa tcttttattc tatattcaaa caaccaattg
     2041 taaaaccaat gctctaaagt tattttatca tctgaaatat cagaacttaa ataatattgt
     2101 tttttataag catcaagttt atctttaact tccttttgag ttttaccata aaattctttt
     2161 cttattggtt ttccatcttc attataacca atcattatac gagaacgcca accatcttta
     2221 tattttccga tagagccttc tccattagct cttttagttg ccatttttaa acaactcctt
     2281 atatgattta tagttatatc catcgaagaa tgtaatacat ttctctaata agaattttat
     2341 aacttcatca gcttctagtg tttcatagaa gtttagcatt aatgtgttgt attcttgttt
     2401 atgtttggct ggtattgata gaactcctat atcattactt ataagtatta ggtttgaggt
     2461 aagtcgagca gttcttttgt taccatcata gaagaattga caataggaca accataaatt
     2521 tattattata gccttttcta aggggttagt tattttattt atgatactta tgtcacttat
     2581 aaataaatta tttaatagag tggcttctat gcactcatat tgagtagtac cagctatacc
     2641 tatgttaccg tttctaaaat ctccaacaat tagagcttca tcttttgcaa ctattttatg
     2701 tatagagcaa attgtatctt tatttaaaga taagttttct ttgttactta attttattat
     2761 ataatcccat gatgatttta tatttagtat ttgattttgg tcacttaatt tatgtccacc
     2821 tactgttata ccatcaagta atgtttgtac ttctgggaat gtgaaggggt tattttctag
     2881 gttacccata tcatatacaa attcacttat cattcttcta atgtatgtta aagtatactt
     2941 tttatctcca tgaggtagat tcgtagggaa tttatgtgtg tattttggta tatatgattt
     3001 attcatgatt ttagctcctt tcatatctaa tttttattta ttaactatga atgtataaaa
     3061 gttaataggt aaacccatat tttgaaaatg tgcaatacta atattcttca tcctcatctt
     3121 caataagacc attagagttt tcttgtataa aattttcaac aagaccattt atcacccaat
     3181 ttagattttt ggagaagtct ttgaagtcag agaagcttaa gctaattggt tttattgtat
     3241 ctgcattatc tgtaatttca aacttttttt tatagtctgt ttttatatca taaccataat
     3301 tttctaatat gtgaactact aaatccaact ctttatcaaa tacttttaaa ttaatattat
     3361 tatttgttaa agtatctaaa tcattttctt caacaatgag tttatttatg gcaattgata
     3421 aagcagtaga tatttttctt atcatatcta gactaggtat tctttgattt tgctcatatt
     3481 tagctatagc atgaactgaa caaccaatct tgttggctag ctcattcctt gacatattta
     3541 tcgatttcct atattgttta atcttttctc cgattgtatt atttatcata attccacctt
     3601 aaatatatca tcaaacaatg attgtatttt tacacatata aaggagttat tttctagatt
     3661 atctatatca tatactaact tactcataat tcttttaatg tatgttagag tatatttttt
     3721 atctccataa aataaattag tagggtattt atatgtgtat tttagcatat atgctttatt
     3781 aataattgta actccatttg tagtttttta tcattgcttt agatagtatt gatattattt
     3841 tatattttag ttagtagttt ttttatttct ttaagtgtat tttcaagctt tttatttttt
     3901 tcctttaatt cattattttc ttgtaataat ttaaagtaat cattattttt aatagtcata
     3961 ttttcttttt gagatattaa cttttttagt cgaatgttac tcccattatc tgttgcctca
     4021 ctaaagtaat catagattgc ttctatattt tcttctgata gaccaatatc taataattca
     4081 tcaattgatt gttttataag aacatcattt ttaataattt cttttatatc atcaggatgt
     4141 aatgaaaaat ctaattcagt attagataca caatctgaaa gtatccaatt ataaacttga
     4201 ttttcattta gttctaattt ttcaccaagt ttataatatt ggtctttatc agaccaagca
     4261 cctagcaata aacttgaggt aatcctaata tcatcatctt ccataagatt tgaaatagca
     4321 tttaaaaatt catcaaaaga atagtattct tctgggaata aagactgtaa aaaatataaa
     4381 acatcttcat tagtcattac ttcattttca atatcaaaat aaaagatagt cttttcaatt
     4441 tttaaagctt tagcgagttt tgataaggta tctatactag gtattctttt atcattttca
     4501 taatttatta gagcatttct tgagatgcca ctaatttcag caagttgaat ctgtgttagt
     4561 tctttttgtt ttcttatctt ttttaagttt tttccaattt gcaatattat cacctcgtat
     4621 aaagtatatc ccaaaagtac acaaatgaga acagaaaaat aatatattct tattgacagc
     4681 acacatatgt cgacattata attaaaatgt aaagtttacg aatgtaaact agaaaaaagg
     4741 aggaaaaagt atgggaatca atttaaaaat atttaggatt aaaaaaggtt taaagcaaca
     4801 agaactggca gatagagttg gtatatcaag atattatttg tcaaacttgg aaacaggtaa
     4861 ggctaataat ccaagcaatg atttgatgat taaactatct agagctttag atacaacagt
     4921 agaagaatta ttttttagta aggaggagta aaatatgaaa gagaataata atttagaagt
     4981 tttaaagaca ttacaagcta ctttaaacat tattgtaaag aatgaagaag aagcacaaga
     5041 aaaaaagcaa ggatataaaa ttattgaaaa tgcgaaaaga aataaagctg aacttataac
     5101 atgtaaagaa gcatctaagc tttatcctat aggagaatca aaatttaggc agttatgtca
     5161 ctctaaaaat aaaggtttcc cttgtatatg gataaataat cgagtataca taataaaaaa
     5221 caaattggat gattggttta ttgaaaatgc aaatggtatc aagttatagt cttaaagtag
     5281 gtgtttgcaa ttgagcaata aagggtggat aaagctttac agggagttgt tggataaatc
     5341 aatttggaaa gatattaaac cagaaagaaa agtaatatta ataactctat tacttatggc
     5401 tagtcataaa gaaaatcatg tttttataga taataaaagg ataaaaataa agtctggaca
     5461 atttatttct tcttatagca aaatagctga aagatgtggt aaaggagtta gttatcaaat
     5521 ggttagaagt tgtttgttgg tttttaaaaa tgctggtttt ctaacatacg aaacaacaaa
     5581 caaatatata gtcataacta ttgaaaactg ggagctttac caagagaaca tagaaaatca
     5641 acaacctaat caacagacga ttaacaatca atcaacaaca atcaagaata taaagaatga
     5701 aaataatatt atatatagtg aaattataag caaatttaac tcaacttgta ttgaactttc
     5761 aaaggtcaag aaactaacag acacaagaaa gaggagaata aattcaataa taagagaata
     5821 tgataaagaa gctgtattag aggttataaa tatagttagt aaaacaagtt ttctgaatgg
     5881 aaataataat acaggatgga aagcaaattt tgattggata atgaattcaa gtaattttac
     5941 caagatacta gagggcaatt atcatagcaa ggaaattgtc ataaataaag agacaggaat
     6001 atataatcca gatgattcac aattaaagtg gtaaggaggt tatattataa ataattcaaa
     6061 caacttaatt aaaaatacag aagcagagca atcaatatta ggaagcattt tattagactc
     6121 aacaatagta gaagagttag agcaaatgtt aaatgaagac atgttttatc atgaggaaca
     6181 taaatacata tttaatgcta taaaagaaat aaataagaga aatgaagtaa tagatataat
     6241 aactcttaca gaagaattaa agagaaaagg tcatttgaat aatattggag gtataagcta
     6301 tattaccagt ttgtcaacaa tagtcccaac agtatcaaat ttcacgagtt atgtcagtat
     6361 agttattgaa aaatataaaa atagaaatat aattgataaa ttcaataagt tcaatcttgg
     6421 agaaatagat gataatactc ttatatatca gttagaaaat gatataaata aagccaatca
     6481 gaaattaata aaaaaggatt caagtataac agccatatca gagagagcct ttgaatatat
     6541 aaatgatgaa atgaatgaag gtattaagac tggaataaag ttcttagatg atactatagg
     6601 aggtttatat ggaggtgaac taactacaat agcagccaaa agtggaaggg ggaaaacagc
     6661 attagcttta caaatactta gaaatgtaat atttcaagga aagaaagtac tttttataag
     6721 tggagaaatg agtgatatac agattctgtt tagaaatata gcctcactaa ctggaatatc
     6781 agttgtaaaa atgaaaaata gaaatctaaa tgatgatgaa atagtcaaat atatacaagc
     6841 tttaagcata ataaatcaag aaaataacct ttatataaat gactctgtaa atacagtatc
     6901 agggataaag agagaaataa agagtactaa gcctagctta gtcataattg attatttgca
     6961 aattttggat tatgaaggta aagaagtagg agaagctaaa tttgctgact tatcaaggca
     7021 aataaaaaag attactttag attttaatat accagttatt cagcttgctc aattaaatga
     7081 tgaattgaag gatgaaagac ctaaagggga tagagtaatg agaagctcaa agcagatata
     7141 tatggattct aattcagtta tatatattca tagacctaca gaaaaagagg ctcttaatta
     7201 ttgcaagaca atgggaattg aggatataag agagttgaga cgaagagaaa aaggttttgg
     7261 tgctaattta agtgagatta ttgttgccaa aaatagagat ggtgagtcag gatttagacc
     7321 atattggtat ataggaaatt tgttttcttt tgaagaatgt aagaagatac aagagatata
     7381 aaaaagaagc atgatttatt aagtaataga taaattatgt ttcaagaagt attgaatggt
     7441 aaatatataa aacttaatta gatgtaatag agatttaaaa tatattgagt ttagatttaa
     7501 atgcttatta gaaaaaggta gttgccatat aaatataaca actaccttta taaattaggt
     7561 acttaatgta cactaaccta gcaacttaag tatatattaa gtacctgatg aaagtcaagg
     7621 aggtactaaa atgtatgatg aaattataga aatatatttt gattattgga taaatgaaca
     7681 tatgtataga agtattttag aaaaagatga gtattatatt gaatgttgga aagaggttaa
     7741 aaaaattaat gagttattgg aaataaattt ggaagatgat aagaaatatc tgttggttga
     7801 attaaatgat ttgtttaatt cattaataga atttgaaaga gaacttagtt ataaattagg
     7861 atttaaaaca ggagcaaaat ttataataga gttgttgaag taattaattt gtgtatctca
     7921 atagaataat taaatagtaa atcgagatta ttaataagtt gtactagaat attttaagtg
     7981 aagtatatat taattatatt gtttaaaata aatatattta ataaagaggg gataagtatg
     8041 ccctctttta tttttatgca atcaatgaca ttgtaggatt tacgtatttt gataaatagc
     8101 acaatatatt ttaataataa atatatgata caataatttt attaaaatct aaaataatca
     8161 attgaggagg agaatatgat aatacctaag ttagagccta atgatagaaa aagagagttt
     8221 aataaatact catcagagca aataaacaaa gtagtttata attatttatt taaaaataat
     8281 atgagtaata gaaagataga tgatgagata ctagatttat catcaaaaca aagtaaaggt
     8341 tatcaatcaa tgggtatagt gcatcattta ggaataaata aaaatcataa aggtattttt
     8401 acaggataca caatagataa agcaattgaa gttcttaagg aagatgagat acatttcaag
     8461 cagattatta atatactttt agaaattaga aatataaatg agtatcctat aatagttgac
     8521 tcttgggagt tattagatga gaaaatagct ttaaaaacaa ttgataaatc tacattaaaa
     8581 cataatggga caggagttcc aatagaaata agatattttt ttgagataaa taatttagga
     8641 aagaatgaaa agaaaaatgt gaattttaaa tataatgata attattatag tgcagttata
     8701 gagatggata aattagagaa tccaagaagt agaataattt ggaaatctaa gtttagagat
     8761 ttactaagaa gtatattttc tgagtattat gagaaattct tagaaaataa aatagaagga
     8821 aaaacaaatt tacctaaaat aagattcata aaacttgata gtaataattt taacatagaa
     8881 ttcattttta ctgctgaaat agaagacgat gtattagagg agataagtca agataatcct
     8941 ttgaacatta atactattaa tatagaatta gaaactgaaa ttagaaaaga agggaaagtt
     9001 aaatatgttt atgggaaaca gtatgagcgt aaccccagaa atagaataga agcaataaaa
     9061 tatcatggca caaagtgtat tgtatgtggg tttgacttcg aaaaaacgta tggagataga
     9121 ggtaagggat acatagaaat tcatcatatt aaaccattaa gttctgttgg agaagaaagc
     9181 aatataaatc ccaaaacaga tttggtgcca atatgttcaa attgtcatag aatgattcac
     9241 aggaagaaag ataatgtttt gagtattgat gatttgaagc aaattataaa agatagtaaa
     9301 taatgcttat gtgtggcaga gatgtataaa gtatatattc taaactagtt aacaacaaat
     9361 actaatataa aaaatcaaaa aatttattag gaaaaggtag ttgctaaagt ataacagcta
     9421 cttttataaa ttaagtattt gaggtatatt agtctaaccg attaggtata tcttaagtta
     9481 ttgatgaaaa ttaattaaga ctaacatgta tgatgaatcc atagaaatgt atttttattg
     9541 ttaaataaat gaacatacat agaaatattt tagaaaatta caagtactat attgaaagtt
     9601 tgaaggagaa ccttgattta aggtaagtgt aaagttttta gaaacttcac taaaataatc
     9661 taattaagtt atatatttgt tacttttagg aaaataatgg taaaaattaa aggtttttat
     9721 aaatgaatgt ctaattttat aaataaaatg gagtatttta actaaatatt tgaggagata
     9781 aaatgatatt aaataaaatc gaaataattg gagatatggc tcactttaaa attcctatgc
     9841 atagcaagat acaaagaaca tatgaaattc caccaatatc aacagttgtc ggaattttaa
     9901 aaaatatata tggtgaagaa atagatgatt ttatattagg atatgttata tatcatgatg
     9961 gtacatttaa agatttacag actatatata aagaaatcaa tccaaatgta aaaacattga
    10021 cagatagcga tagatttcaa actgatgtat gtcccattga atacctaatt aatcctaaac
    10081 ttatcatata tactaacata gataaaaata tagaatttaa tgagccgcta tgtttaggta
    10141 agactaattg tttagcgaag gtattatctt taagtgaaat tgaagtaaat ctaatagata
    10201 aaaaagctat tggatacaat caatatacag atattaatat tggagatgga atgataaaaa
    10261 gaataaatac attgacagag tataatgaaa aaaaaggtta ttatgatata tattcttcaa
    10321 tggtaagaga aaatgatgag tttgaatatg gtaaatatta tgatgaagat gagcaacaga
    10381 atattttttt atggaaatgg aaaaaagggg gagtaattaa atgcatttag taagaagaca
    10441 tttgatactc acagtggata atgaagttgt attagattac aattaccagt atgagctaat
    10501 gaaaaggata tatgaagcta tagaaattaa tgataaaaga aaagcactaa gtcttcacaa
    10561 tgaaggctat aaggtggata agaaagtatt taaattattt aattacacaa ttatgtttga
    10621 aaatgctaag tatttaaaag agggaataca tttaaatccc caaacaaaaa tcaaattaat
    10681 tttaagtgga tatgatgata tattgaataa tattataaaa ggttttatta aatgtaaagt
    10741 atttaaattg aataatatag agtttaaagt ttcagatata gaagaagatt caaagaaaaa
    10801 ttttaataat ataacacttt ataaagtaag gtcacctatt gtagcaagtt tatatgattt
    10861 aaaaagtagg aaacaagtat acttaaatcc aatgcaagaa gagttttata aagcattaca
    10921 tgataattta ggaaataagt ataagcttat acataataaa gaatatactg gtgaactata
    10981 ctttgatatt gaggatgtat tagcagtaaa aaagaaatat ataactaata taaaaggaaa
    11041 aggatttata attggatata cagattttga aatatttgta caagctaata aagatatgca
    11101 agaagtaatt tattattgtg gattaggtga aaaaaatagt ataggaatgg ggttactaac
    11161 ttatataact tctaggaggg cttaatatgg atagaagtta caatgtattt gttgataatg
    11221 gtctatatgt gttggcttat tatctgaaca aagatattaa tgatataact tatcaagata
    11281 tagagaatag tatagattta atgagtgata aaattgaaga atttgtaagt tgcgaaaaat
    11341 actctaattt aaaaagtatg tgctttagca attctacgct aactcaacca aaaggaaaag
    11401 ttactttaaa tgagaagtta aaaggttttg ttaaaaatca aggagatgaa tattgtagtt
    11461 tatgtggtca atataaggcc aaagtcaaaa ttgaagataa agaatacaat atagggagaa
    11521 gttatatgcc taatttggtt gcaaatacat tttataattt ctccaataat ttgcaaggtt
    11581 tgaatgtatg tccatattgt ttagtattga ctatgtattc aattctgaat tgtagagtaa
    11641 gtaggtatgc ttttttatac aattctacat caagcgaatt tatggaagat tatactttcc
    11701 atattcaaaa agaaaattta actgatgtag agttaggagc aaagaaagaa aaagaaaagt
    11761 attcaattgt aaaattatta gaaagtctag tgtgtaaata taacttattt aatggtgata
    11821 tagagcaata tatgtttaat aactctggtc aatctcagga tattcaggta aatagaataa
    11881 aaaataaata tgtgaattta ttaataaaat tacaggaaaa agcactacta tcaaactttg
    11941 aaaagttaca tttggatagg tatatcttga atggtacttt agaaagcaat tatctcagag
    12001 aagtatatag ggtaaaaaag gaagaaaaaa tggatgagaa agaacaaaag aattttgatg
    12061 aacttgttat ttttttaaac agggaggtaa atgtattgaa aaaagtcaaa atagatataa
    12121 taaaaaaatt aacggataaa ttaaaaacta gtgacttaga tatagacaaa atatataagg
    12181 agcttaaaat gttaggaaaa ttttatgcat atgaggactg gttagttaac ttaaatgaaa
    12241 tgtattatga aaaaactgga gaaaaactat ttgaagcaga ggattatatt gagttggatg
    12301 atataagaag ctttagacag attaaaaatt taatattagt aagtttaatt taataatatt
    12361 atggagggtt aaaatggaaa ataaaaattc aaatagaatg ttaaatatag gctatgcaat
    12421 agaagtttta tgtgggcgat taaatggaag tagtagtgaa gtagaaaaga aaaataattc
    12481 atataagaat tctacttata ctaaaaaaat agggagcaga agtagaatct caagtcaatg
    12541 tcaaaaatac aatcaaaagc tatttatgga acaagtttgt ggttatgaac aaaatgttaa
    12601 aactaaagat gggaaacaag taaaattggt tccaaaccca tttaaatatg cgacagatga
    12661 tatatttggg tttatgatgg cggaaaaatt atctatagaa gaagatgtat acaaagaatt
    12721 atcagatgaa gaaaaagaaa tatataaaaa aaataaaaag aaatatgaag taaatataac
    12781 taaaaaaaga aagtcaagat ggcaaatgaa ctctttaata aatgtatcaa atagaaaagt
    12841 tgaatgggag tggaatgttt gtggaactac aggagatagt atgccatatc aagttgaggt
    12901 atattcaggg gtacatacaa atatagcaaa tcttaatata ggaaagatag gagaatatga
    12961 tatatctgat atagcaagtg agcatagaga ttatacaact ttagaagcta aatcaattgg
    13021 agttgaaaag ttagataaag aagaaaagtt taaaagaata gaagctctcc taaaatcact
    13081 agaatactta agtgtagaag gtaataagac aaatcatcta actgatacaa aacccaaatt
    13141 tgttatattg ggagagtatt catggggaaa taatgtattt cagggtatta tcaaagaaaa
    13201 tggcttagat gtagagatgt taaaagaatc tatagaacaa aatgaagaat ttagaaaaag
    13261 cgatatatgg attggaataa ataaatttta tgatgataca tttaatgaga tagaagttaa
    13321 taatgaaaag gaacaaataa agaagtattt agaagaagag tttgaagagt atgattttat
    13381 gaaaatatca aatgtacatc aagcttttga aagttactta aaatatttaa aggagacttt
    13441 ataaaggata tatcatgaat aatttatatg aatataaagc taaatcagat gggacaagta
    13501 tagataagca taatgaagat atgaattaca taatgaatca attagttagc atatataata
    13561 tatctaatga tatgaaaaat gatttatatg aggcaattgt atgtcatgat gtagggaaag
    13621 tagtaaaaag ctttcaagat aatattgaaa gtagcacaag aagagttaga catgagttat
    13681 tatcagcaag tattaagtat ttaaatagag gtcagaagtt agctatatta acccaccata
    13741 aagatataaa gtttctagag ggtcaaataa caaattctat ttatgaaatt gaattaaagg
    13801 aattaaagga actattagat attgaagtag aagatataag accatttata aaggaaatat
    13861 caagagcaag aaatgatgat actagaaaac ttgaaaatat attattaaaa ggttacttgc
    13921 aatattgtga ccatatagct tctgcgggaa ttaaaagtat agataaagga tttaattcta
    13981 ttaaagattt tagtatacca aagggtgtaa gttataattc aatacaagaa aaagttttaa
    14041 atatggataa aaatgaagat ataatgataa tggctatgac aggtcttgga aaaacagcaa
    14101 catcattgta ttggagtaac ctagttcaaa atgaatataa aagtaagaga gtgtattata
    14161 tacttcctta tacagcaagc ataaattctt tatatgaaaa tttatataat agaaatataa
    14221 gcgtaagtat gttacacagt aaagctccat attttttgaa taaaattagt gatgaagaag
    14281 atataaaaca actttaccag atatacaaaa agagtgtaaa acaaattaat gtatgtacta
    14341 tatttcaatt agtaaagtca gtattttctt gtaagagatt tgaaatgcta ctatcacaat
    14401 taaaagatag tatatttata gttgatgaaa tacattgttt tgatacaaga gtattgtgtt
    14461 atacattgga atttttaaaa tatttaaaag aaaacttaaa tgtaaaaata tgtataatga
    14521 gtgcaagtat accaacttgc ttaaaaaagt taatacagga aagattgcaa ataagtacaa
    14581 ttattgaacc agatagtaaa gatttaatat ttagacatag attgaataga ttaagtgaag
    14641 gaatacttga tagcatacat aaaatagaaa atgatttaat gcaaggaaaa agagtattaa
    14701 tatgtgttaa ttcagtagat ttatctcaag agttatatgg aaggcttcaa aaatataatc
    14761 ctaaattaat acatggtcga tttaatacta gagatagaga gcaggcagaa aaagggatag
    14821 aagaaagctt cttattgata ggtactcaaa gtatagaggt aagtttagat ataagttatg
    14881 atgttcttta tacagagtta gctcctttag attcattgtt acaaaggttt ggtagagtaa
    14941 atagaagggg agataatgct gagttgagca atatttatat atatgattat gatagaaagc
    15001 ttaatatata tgatgaagat ataataaata ggactgatat agtaatatca gatataatac
    15061 ataatgataa ttctatagtt gatgagagta aagttccatc ttatttagat aatgtctatt
    15121 tagatataga tatggataaa tataataaac ataaagttgc tttagagatt ataatgaaaa
    15181 atatgaggtt aggggtatgg aataaatctg ctaataatga tatgttaaat aatgatacta
    15241 ttagtgttgt gcctgaatgt cttttagaag agtacaagtt gtatattgat aaaaaaatgt
    15301 atttagaagc gagttcatta tttgtaaatg tatctgtaaa aagatacaat tataataaaa
    15361 agatgtttta tgaatgtgga gatatagttg ttacaagtta tagttataat gataaactag
    15421 gtctaatatt tgattgttaa gagaattata gtataactaa ttaaaatatt ttattgagaa
    15481 tttgttataa tttatatcga ctacaaattt tatatgttac aatagaataa ttttgcagtg
    15541 agcgaaaaat atgtgtaaat ggctgtgagt tagtgatggc aaggtttata actgaatttt
    15601 tgtttttagc taaaaacact actggtgcct cactgcaaat taactatttt aataacccta
    15661 caagtgttgt aattactata gttttatttg gggtttaaat tacactaagt tagttataaa
    15721 tattccggaa agtacttttg ctatttgatt cattctaccc gtttaaatta cactaagtta
    15781 gttataaatt tcactaattt tagttacaac tattacaagt ccagccgttt aaattacact
    15841 aagttagtta taaattatgt gtaatgtatt ttaatgtttt ctagcaacaa tgtttaaatt
    15901 acactaaatt agttataaat tttataaatt ctatgaataa ttttaaagag aggtttcgtt
    15961 taaattacac taagttagtt ataaattgta catttgacat agaagcttca aaatccatcc
    16021 cagtttaaat tacactaagt tagttataaa tgtaacagca cgaatgatgg cacagaccta
    16081 tctgcaatgt ttaaattaca ctaagttagt tataaataga agtacactta caagtaagat
    16141 aaaagaccca ggtacagttt aaattacact aagttagtta taaattctgt agctatatgt
    16201 ggaagtaatt ttagtagaga ggtttaaatt acactaagtt agttataaat tattgaaggg
    16261 gtgagagttg tgactaggca ctacaagttt agattacact aaattagttg taaatttact
    16321 tattcattaa gaaacttaac tgagagtttg atttatatta ttgaattagc aagagtttta
    16381 gaaagtagaa aattataccc caataatacc ccaaccacaa aaataaataa taaaaaatat
    16441 ccaaaaaaat tagtataaca tcataactaa atagctatat acctacactt acgaaaacca
    16501 acaaaacaat caaataacca cttgtagact cccctcgtct ccaccaattt tgaaaatgaa
    16561 aataatagaa taaaaggaat tacttatgtt tggactaagt aattcctttt attttgctta
    16621 aaagcacaca attagcacac aactagaagg ttatataaat aaaaaggctg tcgcactaga
    16681 aaatagtgct acgctctttt ttgtataaaa aaataaatcc caagttcaaa agaacctggg
    16741 attcttgtat aataactgta tgctaaaaca aaatactaca caaattgatt atactactta
    16801 tggtgataat tatcaattta agctaccact aaatattgat tacatgattc caaaagatga
    16861 ttctgtaagg ttattaagtt aaattattga ggagatgaat ttagaaaaat tatataagac
    16921 ttattctcga attagatgaa atagagtaat ccatagacaa tttttgaaag ttataatata
    16981 tacagatatg aactgtattt attcttcgag agaaatagaa agaacatgca aaagagatat
    17041 taattttatg taccttctag gcgaaacctc cactccagac cattcaacaa ttgcaaaatt
    17101 tcgcagtata catttttaac taataggcaa aagcttgatt gcacagttta caaactttct
    17161 tggagataat aatgaaattt caaaagacac aatatttatt gatggtacaa aaatagagtc
    17221 atctgctaat agatatactt ttgtttggaa aaaagctgta aataaaaata tggctaaaat
    17281 ggtgaataat atatctgact tcatattgaa atgtgagatt gatgttggca ttaaaatagt
    17341 ttatgaagat aaaattaaaa tctatcattt aaagaaacta aaaaaaatat caaaagaagc
    17401 aaatgtagca tttattcatg gaaaaggaaa tagaaaatct actaaaaacc ttcatataat
    17461 gggtgaacga aatagttgtt ctaaaacaga taacgatgaa acttttatga gaatgaaaga
    17521 agatcatatg aaaaatgggc aactgaaacc agcatataat atacaatttg gtgtagatta
    17581 agaatatata gtttggctaa attcaggaaa tcaaactgct gatactacaa cattaatacc
    17641 atttttaaaa tctatagagg aatatactaa gtttaaatat cctaaagtaa tagctgatgg
    17701 tggatatgaa agtgaataaa actatgcatt tttagagcat aataatcagt tatctttcat
    17761 aaaacctgca aattatgaaa ttattaaaac tagaaaatat aaaacttata ttggatgtat
    17821 tgacaatatg gattatgaag aagaaaataa ttattatatt tgtacaaatg agaaaaaact
    17881 atttgctaat aaaataataa ataggaaatc aaaaacagga tataaaagtg aaataacatg
    17941 ttacatttgt gaagagtgta gtaattgtca atataaaagc aattgtatta agagaagtaa
    18001 tagtaaggtt cctcttaaaa ataggactaa aaacttacaa gtatctaagt cgtttcatga
    18061 aaaaagaaaa gaaaacttaa agagaattat gagcctcgag ggggatgaac ttagagtgaa
    18121 tagaagtata caagcagaag gagctttcgc tcaagtaaaa taaaatatga aacttacaag
    18181 atttttaagc caaggtaaag aaaatgtttt ataaggatat attattgtgg cgatagctta
    18241 taacgtttct agactacaca ataaaatagt aaaaaataaa cgtggaatat atctttatca
    18301 tttaaaaaaa tattctaaaa ttattattta taatcaaata ttatctgaaa aaatggtatt
    18361 tttatatctt tatttgtcat gcataatttt acttagaaca gaaactttat ataaaacaat
    18421 tgaaaatata cctattaaga taattagaaa agagctctca cagaacaaat ttaatttgct
    18481 catgcaacag ccccatattg atatacattt aggttgtata attatacatt tcaatattac
    18541 atgttcatta ggataatagt tagtaaatga tatgattgag agaatagata taataaacta
    18601 atctagtcct gtattgcaat gtttattaaa gaatatattt ttaaagatcc aagtactgca
    18661 tctcttccag aattgagttg gttaatagtt ttttgatgct atgcaattta taataaaaag
    18721 tataatgatt tagttgatga atcatcagaa tttataccct caaagattaa tcagattgca
    18781 ataaaaataa tgatatattc tatagttata atatctattt tctttgcaac ccctcataca
    18841 atgaatataa atatatcaaa cttggatatg ggtataattc tcatttcagt tttatttata
    18901 cagtttttat ctaaattaat tttgtatgta tattttgata aaagggagat atgtaactaa
    18961 gtggtaatat tggtgaaaac aaaaattaaa gaatatagaa aaaaactcct tatgacacaa
    19021 aatgaacttg ctaaatcagt tagggtaagg tgtgagacta ttgtacactt ggaaaatata
    19081 atccttcttt aaagttagtg atagatattg ctaaagtatt caatactaca gtagaaaatt
    19141 tgtctgaatt tattgaagaa ggatagtgga tagattataa atagttataa gatggttaat
    19201 gaaatatagt tacttaatta ttaagtgtgt tagaaatatt tatatgtaac acaatgtgta
    19261 gttaagatta ccttatctca tctaagactt taatttttct tcccataaag agggtatatc
    19321 aattttctag tgtggtagcc cattttttat tgatttaaat ttataatata tattaataga
    19381 tgatacttaa aaattttata aaatatctaa gttgtcataa ataaaacgat atatctaacc
    19441 gaatatggta aaatttgtta taatatagat aagttagagt aaaaataatt ttaatggggg
    19501 agaaaatgca gtcagtaata tgtaaaattt ttgataataa attactaatt agtaatgatt
    19561 taagtgagca ggatgatgta ttatatgagg taatggataa atttatagat gatattgaat
    19621 atatttcaaa attaggagat gagcaaatta aaagtatgct tatggaggta aataaatttg
    19681 aagaagattc aactgaattt gaactatata tgaaaaactt atgctattta aagcaatcat
    19741 attttataga tactaatgat aaaagtgaa
//
LOCUS       DAMJWP010000053        19277 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.054, whole genome shotgun sequence.
ACCESSION   DAMJWP010000053 DAMJWP010000000
VERSION     DAMJWP010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19277
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.054"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(18..860)
                     /locus_tag="PC700_001597"
     CDS             complement(18..860)
                     /locus_tag="PC700_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2580910.1"
                     /translation="MIIQQIYNNNVVLVLDENIKKELILTGCGIGFQKKKGQEIDKSK
                     IERTFVIQDESFLDKISKLASQVDEKFFEISTEIIAYAEENLNTKLYEYIYVALTDHI
                     AFAIKRYHENITIKNDLLHEIKRIHKKEYEIGKWAVDYINKEFDVKFPVDEAGFIAMH
                     IVNSNYKGSSKESLLITKIVKDILNIIRYYYRVEFKEDDINYDRLLTHLKFFAKRLVK
                     KEKINDTNNEIIDIIKVKYEKDYDCAYKIKTHVEKNYDYYVSQDELLYLTLHIKRVIS
                     VLNL"
     gene            complement(1107..1601)
                     /locus_tag="PC700_001598"
     CDS             complement(1107..1601)
                     /locus_tag="PC700_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDF2580911.1"
                     /translation="MSTLVTLLGLLVCTKISTVFSKKWSNIPLAIYQIVLGIILSILP
                     FKLSFSFNPEIFVICIIAPLLFSEGQNVSRKELLELRKPILLLAFGLVLITVFAGGIF
                     IHFLIPRMPLSVSLALAAVISSTDLVAVKSITQGLNFPKNMMSILEGESLLNDDDRII
                     NIME"
     gene            complement(1753..2724)
                     /locus_tag="PC700_001599"
     CDS             complement(1753..2724)
                     /locus_tag="PC700_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HDF2580912.1"
                     /translation="MKKEESLLKVEGLKKYFEVKSGLFDKEIKCVKAVDGISFDLKKG
                     ETLAIVGESGCGKSTAGRTILRLIEKTEGKVEFNGVDIYKLEKEELRKLRPKMQMIFQ
                     DPYSSLNPRLNVEQIISEAVIEHNLVPKNEIKDNILNVVESCGLSKYHLNRKPDEFSG
                     GQRQRIGIARALALNPEFIVCDEPVSALDVSIQSQIINLLMDLQDKYKLSYLFISHDL
                     SVVNHIAHRVCVMYLGSIIEIATKGELYLHSQHPYTRALMSAVPVQDPTIKKNRIILK
                     GDIPSPTNPPSGCKFHTRCPYCKEICKKEIPVLKEISRNHFVACHLV"
     gene            complement(2725..3702)
                     /locus_tag="PC700_001600"
     CDS             complement(2725..3702)
                     /locus_tag="PC700_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2580913.1"
                     /translation="MSKALIEVKDLKVYFHTDKGIVKSVNEVSFNINEGETIGIVGES
                     GCGKSVTAMSLMKLLPTSKIEGGEIIFRGKDILKMNEDELMVIRGNEISMIFQEPMTS
                     LNPAFTVGSQIIEGIMIHQDLSKEEAKKKVIDMIKLVEIPRAEEIYNSYPHELSGGMR
                     QRIMIAMALSCNPKLLIADEPTTALDVTIQAQILDIMKNIKEKLNTSIMMITHDLGVV
                     AEMCDKVLVMYAGKIIEVAEVVELFKNPKHPYTIGLLKSKPVLGKNKDKRLYSIPGQV
                     PNPIGIPDSCYFSDRCEKVCDKCRTQIPPLIELNSGHSIACWLYKKEEI"
     gene            3921..5480
                     /locus_tag="PC700_001601"
     CDS             3921..5480
                     /locus_tag="PC700_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2580914.1"
                     /translation="MKFKKLASLILVSSLMLTFTACASNDKDKSSGSKSGGTVVIPMA
                     SDPDIINGAFANVKEDSLASNIVYAPLYTYEKGNLVNYLAEKVDFKDSKELTIKLKSN
                     LKWHDGKPITAEDVLFTFNTVLDEKQNSPSRQYLLVGEKPVKVEKIDDLTVKITLPTA
                     SESFLYGISKISPIPKHVFEGESNIAKSEKNNNPVGSGAFKFKEWKKGESIVFEKNAD
                     YFGGEPKADSIALKIIPNEASQEAALNNGEISLMKTSAEGYEKAKSNSNLQTYTYSEE
                     RLNYIVFNQNISNMANKEVRQALSYALNRNEMIESAYGKEGSVPAKSILVPEADFYTE
                     EGVEGYDQDTNKAKDLLDKSGVKIDKLKIGYNTGRFGHKNYALVAQQELKKIGIEAEI
                     VPYESKAFFNILFSNSTECDMYVNGYAWGLEPNPYRGMFETGQYCNQTKYSNAEIDAL
                     WEKGFTELNKEKRKEIYKQIQQDISKDAPIYTIDYEQNLMAAQKNLKGIKDAKPSPAI
                     LFEDWSKLYVE"
     gene            5586..6545
                     /locus_tag="PC700_001602"
     CDS             5586..6545
                     /locus_tag="PC700_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2580915.1"
                     /translation="MLKLILNRVKVLIPMLILISILSFGLLELAPGDPADAYINPLMS
                     SQDIENIRVNMGLDKPVYIRYLNWLKNTLSGNLGISYINHMPVTEQIMEKMGNTFILM
                     GTSLIFSILVAIPLGIFLAVNKNSITSKVSSVFNYIGVSIPSFWIGMILISIFSVKLN
                     IFPSGGMHTIGNDSIGDLVKHLVLPVITLGLYNTAIFTNYVEASVNEQLKKQYVITAR
                     AKGLSEKVILFKHVLKNSLTSLVTILGMSIQKLVTGAFVTEVVFSWPGMGRLMIDSIF
                     SRDYTVIMAITMLSALFLILGNLVADILYLLIDPKIKSSKGGF"
     gene            6548..7360
                     /locus_tag="PC700_001603"
     CDS             6548..7360
                     /locus_tag="PC700_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2580916.1"
                     /translation="MFTSILKKLKKDKSFAFACITLSIIIILIIASFILNIDSNAIDT
                     TSKLQEPSLKHIFGTDELGRDYFSRALYGGRISLSVGILSMLISVVFGTAVGVISGYI
                     GGKLDQFLMRLVDMLMSIPSFLVLIVLNTYLTPKVSTIILIIGFLSWMEVSRIVRGET
                     LKIKENEYCLAAKATGIKTRNILLRHIVLNLKETIVVAGSLNIANAILTESALSFLGL
                     GIQLPLASWGNMLQDAQRHIFDKLYLAVFPGLLIFLTVISLTIISKKFSSNT"
     gene            complement(7575..8477)
                     /locus_tag="PC700_001604"
     CDS             complement(7575..8477)
                     /locus_tag="PC700_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2580917.1"
                     /translation="MDIKQLKYFVEIVKSGFNLSIASKTLHISQPALSQIIKNFEESE
                     DVYLFERYKGRLNGLTPAGERFYVNAEHIINEYKNMMEDLREDSVQFKGKVRIGIPPL
                     ILGIAFSDVVAQLVANNPDIEFDIVEKGAYDLNRMLILQELDYAVLLHPHKIDKSVVT
                     EHVLQEDELTAFLNINHPLAKKDKIDWNDLNEQLLAIFNPTFMIHHKLMQKFTDENVK
                     LKRYIMSGSWDFLLLSTTNSDFITILPSPVHDFFENSKIIEKPFNHPITWKVILCRPK
                     KERYSHVDQHVFKFIIDFFSKKNS"
     gene            8832..10010
                     /locus_tag="PC700_001605"
     CDS             8832..10010
                     /locus_tag="PC700_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDF2580918.1"
                     /translation="MKDVVIVSAVRTPIGSFGGVFKNTSAVQLGTIAVKEAISRVGLN
                     LSEIDEVIIGNVLQTGLGQNVARQIAINSGIPNSVPSYTVNKLCGSGLKSVQLAAQSI
                     TSGENDVVIAGGTENMSQAPYIVPTARFGSKMGDITMVDSMLTDGLIDAFNQYHMGIT
                     AENIATKFEFTREMQDKLALESQNKAENAIKNNRFKEEIVPVDVLIRRGKIETIDKDE
                     YPKLGMTFEGLAKLKPAFKKDGTVTAGNASGINDGAAMLILMSQQKADELGIRPLAKI
                     KSYASAGVEPEVMGTGPIPATRKALKKAGLSINDIDLIEANEAFAAQALAVKNELQID
                     SSKLNVNGGAIALGHPIGASGARILVTLIYEMQKRKVETGLATLCIGGGQGISMVVSR
                     "
     gene            10019..10675
                     /locus_tag="PC700_001606"
     CDS             10019..10675
                     /locus_tag="PC700_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HDF2580919.1"
                     /translation="MNKIVSIDEALSHVKDGDTIMVGGFMANGSPENLIDYLCSKNIK
                     DLTLICNDTGFIDKGVGKMVVNKQFKKIIASHVGLNKETGRQMNNNETDVELVPQGTL
                     AEQIRAAGYGLGGILTPTGIGTLVEEGKQKIVVDSKEYLLEKPIYADVALLFASKVDK
                     AGNLVYKGSMNNFNNLMASAAKITIVEADEIVEIGDIDPNEVNTPGIFVNYIVEGGNL
                     "
     gene            10675..11340
                     /locus_tag="PC700_001607"
     CDS             10675..11340
                     /locus_tag="PC700_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HDF2580920.1"
                     /translation="MDKLEMQEYIANRVSKELKDGAVVNLGIGLPTKVANYIPDNVNV
                     ILQSENGFLGLGSAEDGNSSDETIVNAGGQPVTILPGGCFFDSATSFGIIRGGHVDIT
                     ILGALQVDKYGNIANYMIPGKMVPGMGGAMDLVTGAKKVIVAMEHTSKGSAKILNNCT
                     LPLTATNAVDLIVTEMGVMEVTSDGILLKEINPAFTLDDVISATEAPLILSDSLNGKI
                     SVV"
     gene            11445..12221
                     /locus_tag="PC700_001608"
     CDS             11445..12221
                     /locus_tag="PC700_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HDF2580921.1"
                     /translation="MVKDKVVFVTGAASGIGKQIAESFLKNGAKVMFSDINQEALNNT
                     ITELQKKGYDCMSVKCDVTKEEEINHAIDKTVEKYGRLDILINNAGLQHVSMIEDFPT
                     DKFEFMIKIMLTAPFIATKKAFPIMKKQGFGRIINMASINGVIGFAGKAAYNSAKHGL
                     IGLTKVAALEAADSGITVNALCPGYVDTPLVRGQFNDLSKTRNIPLENVLEEVLYPLV
                     PQKRLLDVQEIADYAMFLASDNAKGITGQACLLDGGYTAQ"
     gene            12283..13617
                     /locus_tag="PC700_001609"
     CDS             12283..13617
                     /locus_tag="PC700_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDF2580922.1"
                     /translation="MSIIAPIGIIIGIIAIIYFSVKEIHITIAAPIATLIVVLLNKMD
                     VVTSMLGADKNNYMGALGNYIMSYFAIFLLGSILAKFMEESGATVSIADFILNKVGSN
                     HPYRVLVAIFIVSFILTYGGISLFVVMFAVIPLARTLFKKLDISWNLIQIPLWLGIAT
                     ITMTIIPGTPAIQNVIPIQYLNTSLTAATIPSIVGSIGCATFGLVYMKYALNKSIRKG
                     ETYATYTSKLEEESLDRELPGFFASIFPLLALVIIAITGSTFGSEFMKKNVIYIALIV
                     AILLSAFLFHKFLSSKIQTLSIGASGSIGPIFATSSAVAFGAVIMAAPGFDVFSKLIM
                     SIPGSPLISLTVLTSTMSAITGSSSGALGIVMPNFADYYLSTGLNPELIHRVATIASN
                     ILTIVPQSGVFLTFLSLSKLTHKTGFKETFIVVAVGSLIAEIIVITLGLLMY"
     gene            14114..15040
                     /locus_tag="PC700_001610"
     CDS             14114..15040
                     /locus_tag="PC700_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HDF2580923.1"
                     /translation="MMRRNIPTKLKTILALGVLTLATASSAVTASASSLENNVNISNK
                     SLVYSNTKYKFSAKPNCSVKPELKPDKDNSCKDKNHNDKNCNNTNKPEDNNNSDSTNK
                     PDNNKPESNKPEDNNNSGSTNKPDNNKPESNKPEDNNNSGSTDKPNNNKPESNKPEDN
                     NNSDSTNKPDDNNNSGSTSENFSAYQKEVVDLVNIERAKAGLNPLTLDSSISNVATKK
                     SQDMIDNNYFSHNSPTYGSPFDMLKKFGISYKTAGENIAMGQKTPKEVVNAWMNSEGH
                     RKNIMNPNFSKIGVGVAQKSGGSIYWTQIFVG"
     gene            complement(15432..18971)
                     /gene="nifJ"
                     /locus_tag="PC700_001611"
     CDS             complement(15432..18971)
                     /gene="nifJ"
                     /locus_tag="PC700_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDF2580924.1"
                     /translation="MAKFMKTLDGNTAAAHVAYAFTDVAAIYPITPSSTMAEVVDEWA
                     SQGRKNIFGQTVNVVEMQSEAGASGTFHGSLQGGALTSTFTASQGLLLMIPNMYKVAG
                     ELLPGVFHVSARALASQALSIFGDHQDVMAARQTGCVLLASGSVQEVADIAPVAHLAA
                     IEGRLPFIHFFDGFRTSHEIQKVELLENEDYASLLNFEAVQAFRDNALSPNHPVARGT
                     AQNPDIYFQTREASNKYYQNIVGIVEKYMEKMSNLTGRKHSLFDYYGAEDAKYVLIAM
                     GSVTETIEETIDYLNAKGEKYGLVKVHLYRPFSMKHFLDAMPSTVERICVLDRTKEPG
                     STGEPLYLDVRDVFYGKENAPMIIGGRYGLGSKDTTPSDIKTVFDNLVSEQPKNGFTV
                     GIVDDVTNTSLTPSEPIKIASKGTIRCKFWGLGSDGTVGANKQAIKIIGDHTEKYAQA
                     YFDYDSKKSGGITMSHLRFGDTPIRATYLIDEADYIACHKQSYVYQYDLLKGLKKGGT
                     FVLNTIWDQAGLEEHLPAHMKQYIAKNDIKFYTVNAVKLGQEIGLGNRINMIMQSAFF
                     KLAEIIPEEDAVKYLKDSIVKAYGKKGEKIVNMNYAAVDAGINALVKVEVPASWENAV
                     DADKGEVKEPEFIKNILRPMTAQEGNNLPVSTFNGIEDGTFPCGTAAYEKRGIAVDVP
                     EWIIDNCIQCNQCSFICPHACIRPVLVTEEELANAPEGFEAKKALGKGLEGLKYRIQV
                     SPLDCTGCGNCADICPAKEKALVMKPIDTQLDTELDNWAFAVNPDEVAPKVDVMPANT
                     VKGSQFRQPLMEFSGACAGCGETAYIKVVTQLYGDRMMIANATGCSSIWGASAPSIPY
                     TCNHEGKGPSWANSLFEDNAEYGFGMYTAVKQIRNKIVDAMTELVSMDICEDAKAVFT
                     EWLDSRNDGEASKVASAKVVELLEKPACDCTDEKAKELVKAIKDRKDYLVKRSQWILG
                     GDGWAYDIGYGGLDHVLASGEDVNVLVFDTEVYSNTGGQASKATPAAAMAKFAASGKK
                     SRKKDLGMMAMTYGNVYVAQVAMGADKNQFIKAVLEAEKHDGPSLIIAYAPCINHGLK
                     EGMGRTVANEAQAVACGYWHLYRFNPEVKAEGKNPFTLDSKEPTASFKDFILAQVRYS
                     AIAKQFPEEADGLFAKAEADAKERYEGYKKLAE"
BASE COUNT         6703 a         3005 c         2736 g         6833 t
ORIGIN      
        1 tattaaacta ctctttatta taaattcagt acagatataa ctcttttgat atgtaatgtt
       61 aaataaagta gttcatcttg acttacatag taatcataat tcttttctac atgagttttt
      121 attttatatg cacaatcata atccttttca tactttactt taattatatc tataatttca
      181 ttatttgtat cattgatttt ttcttttttt actaatcgtt ttgcaaagaa ttttaaatga
      241 gtcaaaagcc tatcataatt tatatcatct tctttaaatt caactctata ataatatctt
      301 ataatattta gtatgtcttt tacaatttta gtaattaaca gtgattcctt tgaacttccc
      361 ttatagtttg aatttactat atgcattgct ataaaaccag cttcatctac tggaaatttc
      421 acatcaaatt ctttatttat ataatctaca gcccatttac caatctcata ttctttttta
      481 tgtattcttt ttatctcatg tagtaaatca ttctttatag ttatattttc atgatatctt
      541 ttaattgcaa atgcaatatg gtcagtaagt gctacataaa tatattcata aagcttagta
      601 ttcaaattct cctctgcata agcaattatt tcagtactaa tctcaaaaaa tttctcatca
      661 acctggcttg ctagtttgct aattttatct aaaaagcttt catcttgtat tacaaaagtt
      721 ctttcaattt tacttttatc tatttcttga cctttctttt tttgaaaccc aatcccacat
      781 cctgttagta ttaattcttt ttttatattt tcatctaaaa ctaatacaac attattatta
      841 taaatttgtt gaataatcat aatcaatatc tctcccaaaa tacaatttcc cttattcata
      901 aaaaaatacc taaattaaga tacaattcac ctgtatacct taacctaggt attgcctgct
      961 taccagtaac aatccagctt gttaattctt atatattgta ttcaatttat ggttttattc
     1021 tattataaat aaatatatat gtcaagtaaa ttattgaaaa ctatttccta ttttttagta
     1081 ttatttttaa catcaaaaat caaattttat tccattatat ttatgattct atcatcatca
     1141 tttaataaag attcaccttc aagtatactc atcatatttt taggaaaatt taatccttgt
     1201 gtgattgatt ttactgctac taaatcagtt gaagatatta ctgctgccaa agcaagtgaa
     1261 acagataatg gcatcctagg aataagaaaa tgtataaaga ttccacctgc aaacacggtt
     1321 atcaaaacta aaccaaatgc aagaagcaaa attggttttc taagctctag taactctttt
     1381 cttgatacat tttgtccttc actgaataat aatggagcta tgatacatat tacaaatatt
     1441 tctggattaa atgaaaatga taatttaaac ggcaaaatag acaatataat ccctagtaca
     1501 atttgatata ttgcaagagg aatattactc cactttttgc taaatacagt tgaaatcttt
     1561 gtacaaacca ataatcccaa caatgttact aaagttgaca taactacctc tcttttcaat
     1621 aataatatta taaattaaac ttatatatca actaacttaa tattaacaac atgaaatccc
     1681 atcttaatca aaaaaatttt cagtttattc tattattctt attatataca tcaaatttaa
     1741 tttttgaaat atctatacta gatggcatgc aacaaaatga tttcttgata tttcttttaa
     1801 aactggaatc tcttttttgc atatttcctt acaataaggg catcttgtat gaaacttaca
     1861 tccacttggt ggatttgttg gagatggtat gtctcctttt aatataattc tatttttctt
     1921 aattgtaggg tcttgtactg gtactgcgct cataagtgct cttgtatatg gatgttgaga
     1981 atgtaagtaa agttctcctt tagttgcaat ttctattata gaacctagat acataacaca
     2041 aactctatgt gcaatatgat ttactacact caaatcatga gatataaata aatatgagag
     2101 tttatattta tcctgtaaat ccataagtaa atttataatt tgagattgaa tagaaacatc
     2161 tagtgcagaa acaggttcat cacaaactat aaattctgga tttaatgcta atgctcttgc
     2221 tatacctatc ctctgtcttt gacctccaga aaactcatct ggttttctat ttaaatggta
     2281 cttagaaaga ccacaacttt ctacaacatt taatatatta tcttttattt cattctttgg
     2341 cactaaatta tgttctataa ctgcctctga tattatttgt tccacattta atcttggatt
     2401 taatgaacta tatgggtctt gaaatatcat ttgcattttg ggtcttaact ttctaagttc
     2461 ttccttctct aacttatata tatctacacc attaaattca actttacctt cagttttttc
     2521 aattaatcta aggatggttc ttcctgcagt tgattttcca catcccgatt ctccaactat
     2581 agccaaagtt tctccttttt ttaaatcaaa agaaattcca tctactgcct ttacacattt
     2641 aatttcctta tcaaataatc cagatttaac ttcaaagtac tttttaagac cttctacttt
     2701 taataaactc tcttcttttt tcaattatat ctcctccttt ttatataacc aacatgcaat
     2761 actatgacca ctattcaatt ctattaaagg aggtatctga gttctacatt tatcacatac
     2821 tttttcacat ctatcactaa aataacatga atctggtatt ccaattggat ttggtacttg
     2881 tcctggaatc gaatacaatc ttttatcttt gttttttcca agaacaggct ttgattttag
     2941 aagtccaata gtataaggat gttttggatt tttaaataat tcaacaacct ctgccacttc
     3001 tataatttta ccagcataca tcactaatac tttgtcacac atttctgcaa ctactcccaa
     3061 atcatgtgtg atcatcatta tagatgtgtt taatttttct tttatatttt tcattatatc
     3121 taaaatctga gcttgaatag taacatctaa agctgttgtt ggttcatcag ctataagtaa
     3181 ttttggatta catgataaag ccatagctat cattattcgt tgtctcatac cacctgataa
     3241 ttcatgtgga tatgaattat atatttcttc agctcttggt atttctacca gttttatcat
     3301 atcgattacc tttttcttag cttcttcctt tgataaatct tgatgtatca taataccttc
     3361 aatgatttga cttccaacag tgaacgctgg gtttaaagat gtcattggtt cttgaaaaat
     3421 cattgatatc tcattccctc ttattaccat aagttcatct tcattcatct ttaaaatgtc
     3481 tttgcctcga aagattattt ctccaccttc tatttttgat gttggaagta acttcatcaa
     3541 agacatggct gttacagact taccacatcc tgattctcca actattccaa tagtttcacc
     3601 ttcatttata ttaaaactta cttcatttac actctttaca atccctttat ctgtatgaaa
     3661 ataaactttt aaatctttaa cctcaattag tgctttactc attattttct cctctcagca
     3721 aaattactta aaataacata caccttttga ttgtatttta taaaaattta aattacaaca
     3781 ttttcttaca ttttattatt tttttctatt aattataata atataatagt taatttctat
     3841 atatattgat ttaataatga ttactattat acaatgaaaa atatgtgtta aatattattt
     3901 tatggaggtt ttataagaat atgaaattta aaaaattggc atcattaatc ttagtttcat
     3961 cattaatgct tacatttaca gcctgtgctt ccaatgataa agacaagtct agtggaagca
     4021 aatctggtgg aactgttgta atacctatgg caagtgaccc agatataatc aatggagctt
     4081 ttgcaaacgt taaagaagac tctcttgcat caaatatagt ttatgctcct ctttatactt
     4141 atgaaaaagg gaatttagta aattatttag cagaaaaagt agattttaaa gatagtaagg
     4201 aattaactat aaaattaaaa tccaatttaa aatggcatga tggaaaacca ataactgctg
     4261 aagatgtctt atttacattt aatacagttt tagacgaaaa acaaaattca ccatcaagac
     4321 aatacttatt ggtgggtgaa aaaccagtta aagtagaaaa aattgatgat ttaacagtta
     4381 agattacttt accaactgca agtgaatctt tcttatatgg tatatctaaa atttctccaa
     4441 taccaaaaca tgtatttgaa ggagaatcaa atatagctaa gtcagaaaaa aataataatc
     4501 cagttggttc tggtgcattc aagtttaagg aatggaaaaa aggtgaaagc atagtatttg
     4561 aaaagaatgc agattatttt ggtggagaac ctaaggcaga ttctatagcg ctaaaaataa
     4621 ttcctaatga agcatctcaa gaagcagctt taaataatgg cgaaatttca cttatgaaga
     4681 catctgctga aggatatgaa aaggcaaaat caaactcaaa cttacaaact tatacttata
     4741 gtgaagaaag attaaactac attgtattta accaaaatat atcaaacatg gctaacaaag
     4801 aagtaagaca agccttatca tatgccttaa atagaaatga aatgatagaa tcagcatatg
     4861 gaaaagaagg ttctgtacct gctaaatcta tattagttcc agaagcagac ttctatacag
     4921 aagaaggtgt tgaaggatat gaccaagata caaataaggc taaagactta ttggataaaa
     4981 gtggtgttaa aattgataaa ttaaaaatag gttataacac aggtagattt gggcataaaa
     5041 actatgcctt agtagctcaa caagagttga aaaaaattgg tatagaagct gaaatagttc
     5101 cttatgaaag taaggcattc tttaatatac tatttagtaa tagcactgaa tgtgatatgt
     5161 atgtaaatgg ttatgcttgg ggattagaac caaacccata tagaggtatg tttgaaactg
     5221 gtcaatattg caaccaaact aaatattcta atgctgaaat agatgcttta tgggaaaaag
     5281 gatttactga gttaaacaag gaaaaacgta aagaaatata caaacaaata caacaggata
     5341 tatctaagga tgcacctatt tacactatag attatgaaca aaatttaatg gcagctcaaa
     5401 aaaatctaaa aggaataaaa gatgctaaac catctcctgc aatattattt gaagattggt
     5461 ctaaactgta tgtagaataa ttgatgtaaa attttatatt attaattaga aaaggagatg
     5521 tctaaaaata atattgtttt attattttaa aacgagacat ctcttttaat taggagaatt
     5581 tacttatgtt aaaattaata ttgaatagag taaaagtttt aatacctatg ttaatcttaa
     5641 tatctatatt atcatttgga cttttagaat tagctcctgg tgacccagca gatgcatata
     5701 taaatccttt aatgtcttca caggatattg aaaatataag ggttaatatg gggcttgata
     5761 aaccagtata tataagatat ttaaactggt tgaaaaacac tttaagtgga aacttaggta
     5821 tttcttatat aaaccatatg cccgttacag aacaaattat ggaaaaaatg ggaaatacat
     5881 ttattcttat gggtacttct ttgatatttt caatattggt agctatacct cttggaatat
     5941 ttttagctgt aaataaaaat tcaataacta gtaaagtatc atctgttttt aattatatag
     6001 gtgtttctat cccttctttt tggataggta tgatactcat ttcaattttt tcagttaaac
     6061 ttaacatttt cccatcagga ggtatgcata ctattggaaa tgatagtatt ggagatttag
     6121 taaaacattt agttttgcct gtaataactt taggtctata taatactgct atttttacaa
     6181 attatgtaga agctagtgta aatgaacaat taaaaaaaca atatgttatt acagcaagag
     6241 caaaaggatt atcagaaaaa gtcatactat ttaaacatgt cttgaaaaat tcactgacat
     6301 cattggttac aatcctaggc atgagtatac aaaaattagt cactggtgca tttgttacag
     6361 aagtagtttt ctcttggcca ggtatgggaa gattaatgat tgattcaatt ttttcaagag
     6421 attatactgt aattatggca ataactatgt tatcagcact atttttaatt ttaggtaatt
     6481 tagttgctga tatactatac ttattaattg accctaaaat aaaatctagc aaaggaggat
     6541 tctagttgtg tttacaagta tcttaaaaaa attaaaaaaa gataaatcct ttgcttttgc
     6601 atgtattact cttagcataa taatcatatt aattatagct tcttttatat taaatataga
     6661 ctctaatgca attgatacta cttctaaact tcaagaacct agcttaaagc atatctttgg
     6721 tacagacgaa cttggtagag attacttctc tagagctctt tatggtggaa ggatttcact
     6781 tagtgttggt attttatcaa tgcttatctc tgtagtattt ggtactgctg taggtgttat
     6841 tagtggatat attggaggta agctagacca attcttgatg agattagtag atatgcttat
     6901 gagtatacca agtttcttag ttttgattgt attaaatact tatctaacac ctaaagtttc
     6961 cactattata ctaattattg gattcttaag ttggatggaa gtttcaagaa ttgtacgtgg
     7021 cgaaacatta aaaataaagg aaaatgaata ctgtttagct gctaaagcta ctggtatcaa
     7081 gactagaaat atactattaa gacatatagt tttaaactta aaagagacta ttgtagtcgc
     7141 tggttcgtta aatattgcta atgctatctt aactgaatcc gctcttagtt ttttaggtct
     7201 tggaatccaa ttacctcttg catcatgggg taatatgctt caagatgctc aaagacatat
     7261 ttttgacaag ttatatttag ctgtatttcc aggattactt atatttttaa cagtcataag
     7321 tttgactata ataagcaaaa aattttctag taatacttaa caaattttat atagaaaata
     7381 aaaacagtga ttatctaaag atatacactg tttttatttt tcgtttaaaa aatacatatt
     7441 ttgtatatta taactttaaa tactactcta ccatgttatg taaagcctat tattgctctc
     7501 aaacatatag atgaaaaact aatagtttat tgtaataaaa attgatttat ttatttgtta
     7561 ttttaataac atttttaaga gttcttttta ctaaagaaat ctataataaa tttaaataca
     7621 tgttggtcta catggctata acgttctttt ttaggtctac atagtataac tttccatgta
     7681 ataggatgat taaaaggctt ttctataatt ttgctatttt caaaaaaatc atgaactggt
     7741 gatggtaaaa ttgttataaa atcagaattt gtggttgata gcaacaaaaa atcccatgaa
     7801 ccagacatta tatatctttt taacttaaca ttttcatcag taaatttttg cattagctta
     7861 tgatgtatca taaaagtagg attaaatatt gcaagtaatt gctcattcaa atcgttccaa
     7921 tctatcttat ccttcttagc aagtggatga tttatattta aaaatgctgt aagttcatct
     7981 tcttgaagca catgttcagt tactacactt ttatcaattt tatgtggatg taacagtact
     8041 gcataatcta gttcctgcaa gataagcatt ctatttaagt catatgcacc cttctctact
     8101 atatcaaatt ctatatctgg attatttgca actaactgtg caactacatc tgaaaatgct
     8161 attccaagta taagtggagg tattcctatt ctaacttttc ctttaaattg aactgaatct
     8221 tctcttaaat cttccatcat atttttgtat tcatttataa tatgttctgc atttacataa
     8281 aatctttctc ctgctggtgt taacccattt aacctacctt tatacctttc aaataaatat
     8341 acatcttcac tttcttcaaa attttttata atttgactta atgctggttg agatatgtga
     8401 agtgtcttag atgctattga taaattaaaa ccagatttca ctatttcaac aaaatatttc
     8461 aactgtttta tatccatttt aaaccccctt ccccaaaagt ataatttaga ttatattttt
     8521 tcttctaatg atattttata ggaaaatcaa cataatttca ttaatatttt aacaaaatat
     8581 ttttctaaat ctactataaa tttttatcct ataaattttt cttatacact atataaaaaa
     8641 ttagacttag acaaaatgct tttttcgccg taatctataa ttagaaacag gaaaacctaa
     8701 agcattagga ataaaagtaa ttgaaatact taaaaacaag cttgtattaa aaaagtactt
     8761 tattatagtg ttccctaatt tctttattca gaaaattttt aattatcatt caagaaaggt
     8821 aactattaga tatgaaagat gtagtaatcg tatcagcagt cagaacacct ataggaagtt
     8881 ttggaggtgt atttaaaaat acttcagctg ttcaacttgg aactatcgct gttaaagaag
     8941 cgatatcacg tgtaggactt aatttatctg aaatagatga agtaataatt ggaaatgttc
     9001 ttcaaacagg gcttggacag aatgttgcta ggcaaattgc tataaattca ggtataccaa
     9061 attctgttcc aagctacaca gtaaataaat tatgtggttc agggcttaaa agtgttcagc
     9121 tagctgccca atcaatcaca tctggtgaaa atgatgtggt aattgcaggt ggtactgaaa
     9181 atatgagcca agctccttat atcgttccaa ctgctcgctt tggtagtaaa atgggtgaca
     9241 taaccatggt agatagtatg ctaacagatg gccttattga tgctttcaat cagtatcata
     9301 tgggaataac tgcagaaaat attgcaacta aatttgaatt tactagagaa atgcaagata
     9361 aattggcatt agaaagtcaa aataaggctg agaatgctat aaaaaacaac cgctttaaag
     9421 aagaaatcgt tccagtagat gttttaatac gtcgaggaaa aatagaaact attgataaag
     9481 atgaatatcc taaattaggc atgacatttg aaggtttagc taaacttaaa cctgcattca
     9541 aaaaagatgg tactgtaaca gctggaaatg cctcaggtat taatgatggt gcggctatgt
     9601 taatactaat gagccaacaa aaagcagacg aactaggtat tagaccactt gctaaaataa
     9661 aatcctatgc ttcagctggt gttgaacctg aagtcatggg tactggtcca atccctgcaa
     9721 ctagaaaagc actgaaaaaa gctggactta gcatcaatga tatagactta atagaagcaa
     9781 acgaagcgtt tgctgcccaa gcattagctg ttaaaaatga acttcaaata gattcatcaa
     9841 agttaaatgt taatggtggt gcaatagcat taggacatcc aataggagca agtggagcta
     9901 gaatacttgt taccttaatt tatgaaatgc aaaaacgtaa agttgaaact ggacttgcaa
     9961 cactttgtat tggtggtggt caaggaatat caatggtggt ttcacgatag gaggaaatat
    10021 gaataaaata gttagcattg atgaagctct atctcatgta aaagatggag atacaataat
    10081 ggttggtgga ttcatggcca atggttcacc agaaaattta attgattatt tatgtagtaa
    10141 aaatataaaa gatttgacac taatttgcaa tgatactggc tttattgaca aaggagttgg
    10201 caaaatggtt gtaaataagc aatttaaaaa gataattgct tctcatgtag gattaaataa
    10261 agaaactgga agacaaatga acaacaatga aacagatgtt gagttagttc ctcagggaac
    10321 tcttgctgaa caaatacgtg cagctggtta tggtctagga ggtatactca caccgactgg
    10381 tattggtact cttgttgagg agggaaagca aaaaattgtt gtcgatagca aagaatacct
    10441 tcttgaaaaa ccaatttatg cagatgttgc tcttctattt gcatcaaagg tcgataaagc
    10501 aggtaatcta gtttataaag gctctatgaa caattttaat aatttaatgg catctgctgc
    10561 caaaattaca atagttgaag cagatgaaat cgttgaaatt ggtgacattg acccaaatga
    10621 agttaataca ccaggtattt ttgtgaatta catcgttgaa ggaggtaatt tataatggat
    10681 aaattagaaa tgcaagaata tattgcaaat agagtatcta aagaattaaa agacggagcc
    10741 gttgtaaacc ttggaatagg tctaccaacc aaagtagcaa attatattcc ggataatgta
    10801 aacgttatct tacaatctga aaatggcttt ttaggtttag gttcagccga agatggaaat
    10861 tcaagtgatg aaactattgt aaatgcaggt ggtcaaccag ttactatact tcctggagga
    10921 tgtttctttg atagtgctac ttcttttgga ataatcagag gaggtcatgt tgatataact
    10981 attcttggcg cacttcaggt tgacaaatat ggcaatatag caaactatat gattccaggt
    11041 aagatggttc caggtatggg tggagctatg gacctagtaa ctggagctaa aaaagttatt
    11101 gtagcaatgg aacatacttc aaaaggttct gcaaaaatat taaataattg tactcttcca
    11161 cttactgcaa caaatgctgt agatttgata gttactgaaa tgggagttat ggaagtaaca
    11221 tcagatggaa ttttacttaa agaaataaat ccagctttca cattagatga tgttatttct
    11281 gctactgaag ctcctttaat attgtcagat tcattaaatg gaaaaatatc tgttgtttaa
    11341 aataattaaa actagaaaat aaaaatctct tagacgtatt acatataaat ttgtattgct
    11401 taaaatatta atataaaaat aaaataaaat tggaggaaat gaaaatggtt aaagataaag
    11461 ttgtattcgt aacaggagct gcaagtggaa taggtaaaca aatagctgaa tcgttcttaa
    11521 aaaatggtgc taaagtaatg ttctctgata taaatcaaga agctttaaat aataccataa
    11581 cagagttaca aaaaaaaggt tatgattgca tgagtgtaaa gtgtgatgta actaaggaag
    11641 aagaaattaa ccacgctata gataaaactg ttgaaaagta tggcagatta gatatattaa
    11701 taaacaatgc tggacttcaa catgtttcta tgatagaaga tttcccaact gataaatttg
    11761 aatttatgat aaaaataatg ctgacagcac cttttatagc tactaaaaaa gcatttccta
    11821 taatgaaaaa acaaggcttt ggtcgtataa taaatatggc ctcaatcaat ggtgtaatag
    11881 gatttgctgg aaaggctgct tataactcag caaaacatgg acttattgga ctaacaaaag
    11941 ttgctgctct tgaagctgcc gattctggta taacagttaa tgctttatgc cctggatatg
    12001 tagatactcc acttgtcaga ggtcagttta atgacctttc aaaaactcgt aatataccat
    12061 tagaaaatgt cttagaggaa gttttgtacc cacttgtacc acaaaaacgc ctattagatg
    12121 ttcaagaaat tgccgactat gctatgttcc tagcaagtga taatgctaag ggtattacag
    12181 gacaagcttg cttactagat ggaggatata ctgcacaata gttattatat ttagggcttg
    12241 aagaaagtat tcttcaggtc cttctactaa ggagggatat ttatgagtat tatcgcacca
    12301 attggtataa ttattggtat catagctatt atatattttt cagttaaaga aattcatata
    12361 actatagctg ctccaatagc aactttaata gttgttctct taaataaaat ggatgttgta
    12421 acatcaatgc taggtgctga caaaaacaat tatatgggtg cacttggaaa ctatataatg
    12481 agttattttg caatattctt attaggctca atacttgcta aatttatgga agaaagtgga
    12541 gccactgtat caatagctga ttttatttta aataaagttg gctctaatca tccttataga
    12601 gttttagttg ctatatttat agtaagtttt atattaacgt atggtggtat aagcttattt
    12661 gtagttatgt ttgcagtcat tccacttgca agaactctat ttaaaaaact tgatatttct
    12721 tggaacctta ttcagatacc actttggctt ggtattgcaa caattactat gactataatt
    12781 cctggaacac cagccattca aaatgttata cctattcaat atctaaatac gtctcttact
    12841 gcagctacta taccaagtat tgtaggtagt ataggttgtg ctacgtttgg acttgtctat
    12901 atgaaatatg ctctaaacaa aagtattaga aaaggtgaaa cttacgcaac ttacacttca
    12961 aaattagaag aagaatcttt agatagagaa ttaccaggat tttttgcaag tatttttcct
    13021 ctattagcac tcgtaattat agctataact ggaagtactt ttggttcaga atttatgaaa
    13081 aaaaatgtaa tttacatagc tttaatagtt gcaattcttt tatctgcttt tttattccat
    13141 aaatttctat catctaagat acagacattg agcataggtg ctagtggttc tataggtcct
    13201 atatttgcta catcttctgc agttgcattt ggagcagtaa taatggctgc acctggtttt
    13261 gatgtattta gcaaacttat aatgtcaata ccaggtagtc cacttataag tcttactgtt
    13321 ttgacttcaa caatgtcagc aataacaggg tcctcttctg gtgcattagg aatagtaatg
    13381 ccaaactttg cagattatta tttaagtact ggtctaaatc cagaattaat tcaccgtgtt
    13441 gctacaatag cctcaaatat actaactata gttccacaaa gtggtgtttt tcttacattc
    13501 ttaagcttat ctaagttaac acataaaact ggatttaaag aaactttcat tgtagttgca
    13561 gttggttcac taatagcaga aattattgta attacattag gattactaat gtattaatat
    13621 aaaacaaatt aaaattcata atatgaaagg tggtgtctca aagtgaactt tttacttaat
    13681 gagacactct ttttgtatga aatcaaatat attttaatca tttcaacaat aaaaaagaac
    13741 ctttctctat tttgagacaa acccttttta ttccttaaag atttaaacaa ttataatttg
    13801 ttactaatac gaaaacatct attgaataaa tttaaccaaa taaaaaaaaa tttatcataa
    13861 ctataaattt acgttatgga attattataa aaatattatt ttctctaagt aagtaattcc
    13921 atactataca ggcttttaat acaaattttc aaacacactg aaatatacct atttattcca
    13981 tatatatctt ttttacatat ctttacagta aaaaaagtct taatcttatg ttagtataga
    14041 ttcacgggta ccccctacac taaaggttac aaatcagtaa catttttaga atttttagat
    14101 taagaggtgt ttaatgatga gaagaaatat accaacaaaa ttaaaaacta tattagcttt
    14161 aggagtgtta actttagcaa cagcttcaag tgctgttact gcaagtgctt catcacttga
    14221 aaataatgta aatataagta acaaaagttt agtatatagt aatactaaat acaagttttc
    14281 tgcaaaacca aattgttctg taaaaccaga acttaaacct gataaagata actcttgtaa
    14341 ggataagaat cataatgaca agaattgcaa caacactaat aaacctgagg acaacaataa
    14401 ttcagactct actaataagc ctgataacaa taaaccagag tctaataaac ctgaggacaa
    14461 caataattca ggctctacta ataaacctga taacaataaa ccagagtcta ataaacctga
    14521 agacaacaat aactcaggct ctactgataa acctaataac aataaaccag agtctaataa
    14581 acctgaagac aacaataatt cagactctac taacaaacct gatgataata acaactcagg
    14641 ctctacaagt gaaaattttt cagcttacca aaaagaagtt gtcgacttag taaatataga
    14701 aagagctaaa gctggattaa atcctcttac tcttgattct tctatatcaa atgttgctac
    14761 aaaaaaatct caagatatga ttgataacaa ctatttttca cacaattctc caacttatgg
    14821 ttctccattt gacatgttga aaaaatttgg aataagctat aagacagcag gtgaaaatat
    14881 agctatgggt caaaaaactc ctaaagaagt tgtaaacgca tggatgaatt ctgaaggtca
    14941 cagaaaaaat ataatgaatc caaatttcag taaaataggt gttggagtag cacaaaaaag
    15001 tggtggttca atttattgga cacaaatctt tgtaggataa aatactcttt ttccatgcat
    15061 atcaaaagag tatctctaag tgataaaaaa cacaaatgag atactctttt gtatctattt
    15121 taaaattatc aaatccaaat atacatacta taaatattat ttgttctctt aattttagtg
    15181 tgtagaacaa tatataaaaa gtattggcaa gatgaaaatg gaaaacttaa aaagatattt
    15241 aataggtaaa aacaggatct taatatcttc ataaaaaaag gctgtctcaa aatagagata
    15301 aggccttttt cattgttgaa atgattaaaa tatatttgat ttcatacaaa aagagtgcct
    15361 catgaactaa aaagctcact ttgagacact ctcttttatc aattctatat aagcattata
    15421 atactttaat attattcagc taattttttg tatccttcat atctttcttt agcatcagct
    15481 tcagctttag caaataatcc atcagcttcc tctgggaatt gtttagctat agcagaatat
    15541 cttacttgtg caagtatgaa atctttgaag cttgcagttg gttcttttga atctaaagtg
    15601 aatgggtttt taccttcagc ttttacttct ggattgaatc tgtataaatg ccagtatccg
    15661 caagcaacag cttgtgcttc attagccaca gttcttccca taccttcttt taatccatgg
    15721 tttatacatg gagcataagc tattattaga gatggcccat catgtttttc agcttctaaa
    15781 acggctttga taaattgatt tttatcagcc cccatagcaa cttgagctac atatacgttt
    15841 ccatatgtca tagccatcat tcctaaatct ttctttctag attttttacc tgaagcagca
    15901 aacttagcca tagcagcagc tggagtagct ttagaagctt gacctcctgt atttgaatat
    15961 acttctgtat caaatacaag tacgtttaca tcttcaccag atgcaagaac atggtctaat
    16021 ccaccgtaac caatatcata tgcccaaccg tctccaccta gtatccattg agatctctta
    16081 actagataat cttttctatc ttttatagct tttactaatt ctttagcttt ttcatctgta
    16141 caatcacaag ctggtttttc taataattca acaaccttag cacttgctac tttagaagct
    16201 tctccatcat ttctagaatc taaccactct gtaaatactg ctttagcatc ttcacagata
    16261 tccatagaaa ctaactctgt catagcatct actattttat ttcttatttg cttaacagct
    16321 gtgtacatac caaatccata ttcagcatta tcctcgaata aagaatttgc ccaagatgga
    16381 cctttacctt catggttaca agtataagga attgatggtg cagaagctcc ccatatagaa
    16441 gaacatccag tagcattagc tatcatcatt ctatctccat ataattgtgt aactacttta
    16501 atgtacgcag tttctccaca tccagcacat gctcctgaaa actccattaa tggttgtcta
    16561 aattgacttc ctttaacagt atttgcaggc ataacatcaa cttttggagc tacttcatca
    16621 ggatttactg caaatgccca attatctaac tctgtatcaa gttgagtatc tattggcttc
    16681 ataactaaag ctttttcttt agctggacat atgtcagcac agtttccaca tcctgtacag
    16741 tcaagtggac taacttgaat tctatatttt aatccttcta atcctttacc taatgctttt
    16801 ttagcttcaa atccttctgg agcattagct aattcttctt cagtaactaa tactggtctt
    16861 atacatgcat gaggacatat aaaagaacat tggttacatt gaatacagtt atctattatc
    16921 cattcaggaa catcaacagc tataccacgt ttttcataag ctgcagtacc acaagggaat
    16981 gttccatctt ctataccatt gaatgtactt actggtaagt tgtttccttc ttgtgcagtc
    17041 attggtctta gtatattttt tataaattct ggttctttaa cttctccttt atctgcatca
    17101 acagcatttt cccaagaagc tggaacttca actttaacta aagcatttat tcctgcatca
    17161 acagcagcat agttcatatt aactattttt tcaccttttt taccataagc tttaactata
    17221 gaatctttta agtatttaac agcatcttct tctggtatta tttcagctaa tttaaagaat
    17281 gcagattgca ttatcatgtt tattctgttt ccaagaccta tttcttgtcc tagtttaaca
    17341 gcattaactg tatagaattt tatatcattt ttagctatat attgtttcat atgagctggt
    17401 aaatgttctt caagtcctgc ttggtcccat atagtgttaa gtacaaatgt accacctttt
    17461 ttaagacctt ttaataaatc atattgataa acatatgatt gtttatgaca ggcaatataa
    17521 tcagcttcat ctattaaata agtagctctt attggagtat caccaaatct taagtgagac
    17581 atagttatac caccagattt tttagagtca taatcaaagt aagcttgagc atatttttca
    17641 gtatggtcac cgattatttt gatagcttgt ttattagctc caacagtacc gtcagaacct
    17701 aatccccaga acttacatct tatagttcct ttagaagcta tttttattgg ctcagatgga
    17761 gttaaagaag tattagttac atcatcaact atacctactg taaatccatt tttaggttgt
    17821 tctgaaacta agttatcaaa aacagttttt atatctgaag gagtagtatc ttttgaacct
    17881 aatccgtatc ttccacctat tatcatagga gcattttctt ttccatagaa tacatcacgt
    17941 acatctaagt ataatggttc accagttgaa cctggctctt tagttctatc aagtacacat
    18001 attctttcaa cagtagatgg catagcatct aagaaatgtt tcattgagaa tggtctatat
    18061 aaatgaactt taactaaacc atatttttct cccttagcat ttaagtaatc tatagtttct
    18121 tctattgttt ctgttactga acccatagct attaatacgt atttagcatc ttctgctcca
    18181 tagtaatcaa ataaactatg ttttctacct gttaaattac tcattttttc catgtatttt
    18241 tcaactatac ctactatatt ttgatagtat ttattagaag cttctctagt ttggaaatat
    18301 atatctgggt tttgagcagt accacgagca actggatgat ttggagataa agcattatct
    18361 ctaaatgctt gaactgcttc aaagtttaat aaacttgcat aatcttcatt ttctagtaat
    18421 tcaacttttt ggatttcatg tgaagttctg aatccatcaa agaaatgtat aaatggtaat
    18481 ctaccttcta ttgcagctaa atgtgcaaca ggtgctatat cagctacttc ttgaactgaa
    18541 ccagaagcta ataatacaca tccagtttgt ctagcagcca taacgtcttg gtgatcccca
    18601 aatattgata acgcttgaga tgccaaagca cgagcactta catggaatac tcctggtaat
    18661 aactctcctg caactttata catgttaggt atcattaata ataaaccttg agatgctgta
    18721 aaagtagaag ttaaagctcc tccttgtaaa gaaccgtgga atgttcctga tgcacctgct
    18781 tctgactgca tttcaacaac attaactgtt tgtccaaata tattttttct tccttgtgat
    18841 gcccactcat caactacttc agccatagtt gaagatggtg tgattggata tatagctgct
    18901 acatctgtaa acgcataggc aacatgagct gcagctgtat ttccatcaag tgttttcata
    18961 aacttagcca taatatgtat cctccttgat agctacaatt gtacttgttt ataacaagtc
    19021 tattttttta tatttacata taatttacac aacaaaaata taaacacaca tatagtcgcg
    19081 caaatactct atcttctaat ataatactac agattagata aaattccttt gaaaattgta
    19141 aattaattac atatttaagt agattttttt taaaattaaa tatgttaagt ttttttcata
    19201 attttgtata caatatataa taaattaagt tagacttttc aaactttttt atctatttgt
    19261 ttacattagt ataaaaa
//
LOCUS       DAMJWP010000054        19271 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.055, whole genome shotgun sequence.
ACCESSION   DAMJWP010000054 DAMJWP010000000
VERSION     DAMJWP010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19271
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.055"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(13..1206)
                     /locus_tag="PC700_001612"
     CDS             complement(13..1206)
                     /locus_tag="PC700_001612"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HDF2580925.1"
                     /translation="MFNTIEEAIEDIKNGKMVIVVDDESRENEGDLLMAAEMATPESI
                     NFMATYGRGLICLPATEKKFKSLNIPLMVRENTDTFQTAFTVTIDGADTLTGISAYER
                     AETVKLFCDENSTSKDFKTPGHIFPLIAKTGGVLVRDGHTEASVDLARLAGFKEIGLI
                     CEIMKDDGTMARVDDLMIFKEKHNLKIITIKDLIEYRKINETTIEKVSSAFLPTKYGN
                     FEIIGYRDTYSNEEHIALTYGNINIENTLVRLHSECLTGDVFHSLKCDCGLQLESSMK
                     KIVENGSGVLIYMKQEGRGIGLLNKIKAYKLQEEGYDTVEANLMLGFEEDMRDFYMAA
                     QILKNLNIKSINLLSNNPDKINQLEKYGIKIENRIPINEEINDFNKLYLKTKKDKMGH
                     LLDII"
     gene            complement(1229..1894)
                     /locus_tag="PC700_001613"
     CDS             complement(1229..1894)
                     /locus_tag="PC700_001613"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HDF2580926.1"
                     /translation="MFTGIVEEVGILRKITANGKSGKVTILSNKILDGTNLGDSIAVN
                     GVCLTVSNLGKNEFTADVMMETIRSTNLGLLNANDKVNLERAMSLSSRFGGHIVTGHV
                     DGKGTICKFEKDENAVLVSIRPDKKLLSSMILKGSVAIDGVSLTISYLDDEIFKVSII
                     PHTKINTILLTKNVGDFVNLESDVIGKYVNNFMANNYKELNSNSSNHKSNIDKDFLFK
                     NGF"
     gene            complement(1928..3031)
                     /gene="ribD"
                     /locus_tag="PC700_001614"
     CDS             complement(1928..3031)
                     /gene="ribD"
                     /locus_tag="PC700_001614"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HDF2580927.1"
                     /translation="MNQKEKDIYYMKKAIELAKNGEGFVNPNPLVGCVIVKDSNIIGK
                     GYHEKFGSNHAEVNAINSAKQSLKDSTLYVNLEPCSHYGKTPPCVDKIIQNKIKRVVI
                     STLDPNPLVCGNGVKKLKDNNIDVTVGILEDEARDLNEAFFYHIKNKRPLCIVKSAVS
                     LDGKIATKSLESKWISNESSRYLTHKYRNKYQSIMVGINTVLNDNPLLTCRLNQEKVS
                     HPTRIVIDTHLKLPLNSNLVKDKTSKTIVFTCCKESIKLSMLKENNVETIISPSKNNL
                     VDLEFVMYKLGELNIDSVLVEGGATLNDSLFRNKLVDKVKLFLSPKIIGGKDAPTFVS
                     GEGINHLSDSTQLTINNVTLIDGDILIESDVLN"
     regulatory      complement(3163..3284)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            3473..5956
                     /gene="recQ"
                     /locus_tag="PC700_001615"
     CDS             3473..5956
                     /gene="recQ"
                     /locus_tag="PC700_001615"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HDF2580928.1"
                     /translation="MNKKLLEILSKYYGYTSFRKGQESIINSILSEKDVLAIMPTGGG
                     KSICYQIPALILDGMTIVISPLISLMKDQVDALKTMGISAAFINSSLSSKEFNEILNN
                     IRKNNYKILYIAPERLDAQEFIELINNNNVSQVAIDEAHCVSQWGHDFRLSYRRISDF
                     IKNLPKRPIVTAFTATASEEVRTDIINLLCLENPDYYITGFDRENLSINIVKSSSKNK
                     YILDYIQNHKNESGIIYVATRKEVENIYNGLSKRNISVSKYHAGLSKNERSKNQEDFI
                     NDNVDIMVATNAFGMGIDKPNIRWVIHYNMPQSIENYYQEIGRAGRDGEKSECVLLFS
                     PGDVHIQKYLIDIGVENPERKLFQHKKLQYMIDLVYSNSCYRKTILSYFGENYIDNCN
                     NCSNCLVEGEIVDKTIDAQKVISCVARMKRSYGVTTIVDVLRGSKNKKILQLGLNTLS
                     TYNIMRDYSSEDLKNFVNTLVSHGFLDLVETLGNGNRGSFPTIRLNEMSMKVLKGEVK
                     VEFKEIVMTKSLEVEDELYNALRELRHSIASEERIAPYMVFGDGTLRAMSSSYPINKE
                     EMLDISGVGEIKYQKYGRDFEAVIKEYVEKNNINKKIDEKNVDLKNVNSDFLEVNTDK
                     VLYEKLLVIREEYATKEKLPAYMILANKALKEISGRYPLDEEQLKDISGIGGVKFEKY
                     GANILAEVNKYVKENNINVIWEKKGKRKLILDGESRKNNEIALDLLNQGKDIQSISQE
                     IEVSLSTIIGYVHDYVKDGHSISFELGLENFYNEDKKKMILEVCEKVGYDNLNNIKRK
                     LPDSIKYENIRAVMLDSYLENLKNNIIAE"
     regulatory      6044..6155
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6297..7598
                     /gene="thiC"
                     /locus_tag="PC700_001616"
     CDS             6297..7598
                     /gene="thiC"
                     /locus_tag="PC700_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HDF2580929.1"
                     /translation="MNYTTQMDAARKGIITKEMEIVSQKEQVDVNELRELIANGQVVI
                     PANKNHKSLSAEGVGKNLRTKINVNLGISRDCKDIEKELEKVRVAIDMKAEAIMDLSN
                     YGKTREFREKVVEMSPAMIGSVPMYDAVGYLEKELKDITEEEFLNVIRQHAIDGVDFI
                     TIHAGLTRSVCQKIKNHERLTHIVSRGGSLLFAWMELNNKENPIYTNFDKILDICEEY
                     DVTLSLGDACRPGCIKDSTDGVQIQELVVLGELTKRAWERNVQVMIEGPGHMAIDEIE
                     ANVVLEKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGALACAKGVDFLCYVTPAEHL
                     RLPNLDDMKEGIIAAKIAAHAGDIAKNVKGAREWDNKMSKARADLDWCEMFRLAIDPE
                     KAKRYRDESTPTHEDSCTMCGKMCSMRTVKKILNNEELNLI"
     gene            7652..7846
                     /gene="thiS"
                     /locus_tag="PC700_001617"
     CDS             7652..7846
                     /gene="thiS"
                     /locus_tag="PC700_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HDF2580930.1"
                     /translation="MKVNGKEIEFEKDLTVIDLLNKYNLKSDRVVVEVNLEIIEESNY
                     NTYVLKDEDIVELISFIGGG"
     gene            7839..8642
                     /gene="thiF"
                     /locus_tag="PC700_001618"
     CDS             7839..8642
                     /gene="thiF"
                     /locus_tag="PC700_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HDF2580931.1"
                     /translation="MVKIKIFVNELLTDVEENTTAYKVRDSYNNKCDVLVLNGYPIKT
                     DMPLCENDKLTLIQKGVKPSLDELESLLIARHTPNIHNKLKKGKVAILGLGGLGSNIA
                     ISLARIGVGELLLIDYDVVEPSNLNRQQYFIDDIGKLKTDAMIENIKKINPFIKLNKR
                     DIFLNKNNMDTIKDSDLIIEAFDDASCKAQVCNYVLINLKDKYLIASSGMAGYYDSNI
                     ITTKKIKDRFYICGDFVNEAKFGEGLMAPRVAICANHMANLATQILIDM"
     gene            8705..9475
                     /locus_tag="PC700_001619"
     CDS             8705..9475
                     /locus_tag="PC700_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HDF2580932.1"
                     /translation="MDKLVLGGHEFNSRLLVGTGKYGSNNILPEVIKESGSEIITMAL
                     RRVDLDNKQENILTYIPKEMTILPNTSGATNAEEAVRIARISRKMGCGDFIKIEVISD
                     TRYLLPDNEETIKATKILADEGFIVLPYMTPDLYAGRRLIEANAAAVMPLGAPIGSNR
                     GLQMKEMIRIMIDELDIPIIVDAGIGKPSQAMEAMEMGADAVLVNTAIASAGDPVQMA
                     RAFKLAVEGGREAYIAKTGNVSEFANASSPLTGFLGNL"
     gene            9492..10595
                     /gene="thiH"
                     /locus_tag="PC700_001620"
     CDS             9492..10595
                     /gene="thiH"
                     /locus_tag="PC700_001620"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HDF2580933.1"
                     /translation="MSFYDVIEKYRDFDFDGYLNNVTDNDVLRSLSKDKLEDFDILNL
                     LSKTAVKHLEDMAQKAHKLSVQYFGKTVCLYTPMYIANYCVNQCVYCSYNIKSGIKRK
                     KLTMDEIREEGEAISKEGFKHLLVLTGESSFHSSVEYIGEAIEILREKFPSIGIEVYP
                     MEVEEYKYIVDKGVEGLTVYQETYDEEIYKRVHIKGPKSNYEFRLDAPERGAKAGMRT
                     LSIGALLGLNDFRKETFFTILHGKYLKTKYPHIELSYSTPRMRPFKGCFEELVDISDT
                     DLVQAMVCMRLFDPHAAINISTRENLEMRSHIIPLGVTKLSAGVSTDVGGHSQDEHDT
                     AQFKINDESTVKDVEKMLNSIGYQHVFKDWERF"
     gene            10596..11285
                     /locus_tag="PC700_001621"
     CDS             10596..11285
                     /locus_tag="PC700_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDF2580934.1"
                     /translation="MYLITNRKLCSEERYLEVIKESILSGVENIIIREKDLEYQELRK
                     LYMKIKTKINCIDFQEQISDESLKTNINQKECRNKFKVNFIINSNIEFFEKVDCQGIH
                     LPFKLFLNLIENKYNFNENKILGLSLHKVEEVDYLEKLIRNQNIKIDYITLSHIYETK
                     CKEGLNPKGIELLKEAKKITDIKIIALGGILPSNVKETLKYCDDFAIMSTIMRSKDIK
                     KTISNYNEKLN"
     gene            11473..12867
                     /gene="yfcC"
                     /locus_tag="PC700_001622"
     CDS             11473..12867
                     /gene="yfcC"
                     /locus_tag="PC700_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDF2580935.1"
                     /translation="MTDSKKKRFIIPHTFTIIFLLIVLMAILTWIVPSGEFSREDVEG
                     RMVVVPGTYQSVESNPQGFADVFKAPIEGFIDSAEVVGFVLIVGGAFGIVNRTGAIEA
                     GIASVISKFKGKELLIIPISMILFGLGGTTFGMAEETLPFYMIFVPLMTSLGYDSLTA
                     IAIVFIGAAAGAAASTINPFSVGIAQALAQLAPGSGVTYRVVIFIAYILISIAFVMYY
                     AKKVKENPESSIVYEIDKNNKSILNQTSTEIKEFTFKEVSVLAIFGIGMAIMIWGVLS
                     QGWYIPEISMCFAAIGVLSGLVGRLSQIEISESFIDGAKDLASAALAIALARGIVIIA
                     QNGFIIDTILNSAAAFLGGLPKVIFVYLSFFLEAALAFLIPSSSGLASLTVPVLAPLG
                     DLVNISAQVIVTAYQFGVGLTNFITPTSGVLMGALAVAHIPFSKWVRFIMPILLILTA
                     VSLVFLTIGIYIGF"
     gene            13384..14751
                     /locus_tag="PC700_001623"
     CDS             13384..14751
                     /locus_tag="PC700_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HDF2580936.1"
                     /translation="MGLKEFDFIEESIDMLRTMSPTLETISDEIEEYFENILDEKNQE
                     YINVTSRIKSESSLREKIIRNRYLKKYGEASNLIHNVSDLIGLRIECRFIEDENKIYR
                     LLRRYFNKTDDKINYYNKENKNIKLKLSERQPNKQKNGFEIYKIDGVFAYLDREVKFE
                     LQIKSLVNVFWSEIEHKIIYKNNTYLLADKFIKDMMDSIKNNLTMIDNQLLSIYKNFH
                     SGKSFNMKVSKKEIEKLFAKLVYDAFSEKMNKSIGFVVDFKKPCETILNYSFNKQDIS
                     DEVLGNFMLDEFARLNEIVNKDIDFNEQIEFEREPTFDDKFCKDLGNHFRSRLNTEFP
                     WNLFFRILFEIEPYNNTKDFENFVEFIKENVLLEENREQLLCQFEEDSKLIIEDIYDC
                     IFNSISEIDSVEILYNYNLEKINFTSSEIINCICREYECYGEYLEEKEKLMNTFKEKI
                     IQIFE"
     gene            14776..15384
                     /locus_tag="PC700_001624"
     CDS             14776..15384
                     /locus_tag="PC700_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HDF2580937.1"
                     /translation="MRHFSNIYELSKIILEEAICIEKTGVILAGGKNSRMGRDKAFLE
                     LHDKLFIEIAIEAFKNFDELIIISNNEELYSKYDIKVYNDIVKDVGPIGGIYTALEYA
                     KYDIVTIACDMPYLNNQIVERIANKMNDKSVISVTNGKLQPLCSGYKKSIINKVSLCI
                     RENDLKLRSFIDKIDKSYIYFDEEDLFLNVNTVDEYEKLTKV"
     gene            15432..15803
                     /locus_tag="PC700_001625"
     CDS             15432..15803
                     /locus_tag="PC700_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDF2580938.1"
                     /translation="MNLLKCDSWAVILNNSDKESKAYKILDELKRNMYKVVAIDEEKK
                     PIEGIDVYECLKDVPHNIDVVAIIDKQSKMDVILEEVELLDIQNMWFEKGSFSEMMIK
                     KTKDLKLNIEYNLSLYDELTR"
     gene            15982..16320
                     /locus_tag="PC700_001626"
     CDS             15982..16320
                     /locus_tag="PC700_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HDF2580939.1"
                     /translation="MLIVTTEKVEGKKISKVLGLVRGSTIRAKHVGKDIGASFKNLVG
                     GELTGYNEMLTEARQIAIGRMVEDAEAKGANAVIAFRLSSASVMQGAAEMLAYGTAVV
                     LEDDNSILEK"
     gene            16787..17290
                     /locus_tag="PC700_001627"
     CDS             16787..17290
                     /locus_tag="PC700_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HDF2580940.1"
                     /translation="MFNVAIITLSDKGYEGKREDITGKKLTEFVENTGAYKVTEYVLI
                     KDDKEMLKENIIRLCNSNKIDLILTNGGTGFSKRDITPEATKEVLEKEIPGLSEYMRM
                     KSTEITKKAILSRGVSGIRNNSIIINLPGSPKGAVENLSFIIDVLDHGIEVLRGEATE
                     CATKKSE"
     gene            17337..18296
                     /gene="moaA"
                     /locus_tag="PC700_001628"
     CDS             17337..18296
                     /gene="moaA"
                     /locus_tag="PC700_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HDF2580941.1"
                     /translation="MIDKYGRKIDYLRISLTDKCNLRCVYCMPPDVKFDKNYINENLS
                     FEDYKFIIKAMAEQGITKIRFTGGEPLLYPKLNELIKFTKEECGINNIGMTTNAIGLS
                     DRIFELEKCGLNKVNISLDSLKEYKYKSVTRGGNLKDVLKSIESCLNLGIKVKLNCVA
                     ISGFNDDELKDFMFLTVKSPIDVRFIELMPLGEANRVYKKGYFNVKEMIEKIYGLYKV
                     KNDEKSTAEYYMFKGAKGRIGIITPLSCSFCNTCNRIRLTSSGAIKLCLHSKEEIDIK
                     PFLHKPLLFRESMKDIIKQKPEKHHLIENKCSDTNRRMYEIGG"
     gene            18300..18770
                     /gene="moaC"
                     /locus_tag="PC700_001629"
     CDS             18300..18770
                     /gene="moaC"
                     /locus_tag="PC700_001629"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HDF2580942.1"
                     /translation="MLTHINEKGYAKMVDISEKDDTKRTAVATATITLSKEALEKVKN
                     GQIKKGDVLSVAQVAGIMGAKNTSSNIPMCHQIKLVGCDLEFDVDDYMSAIKIYATCK
                     SLGKTGVEMEALNACSLAALTIYDMCKAVDKKMVISNIHLVEKTGGKSGDFKFE"
     gene            18806..19237
                     /locus_tag="PC700_001630"
     CDS             18806..19237
                     /locus_tag="PC700_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HDF2580943.1"
                     /translation="MAKVVSINISEKKGVIKVPVPCAELKINHGIVGDAHAGNWHRQI
                     SLLGKESIDKMKMKGFDQLKYGDFAENVTTEGIEVFSLPVGTKLRIGQCEVEVTQIGK
                     KCHNGCEIKKLTGDCVMPREGIFVKVLKEGIINVNDSIDVI"
BASE COUNT         7522 a         2067 c         3346 g         6336 t
ORIGIN      
        1 tcatataatc atttatataa tatctaaaag atgtcccatt ttatcttttt tagtttttaa
       61 atatagctta ttaaagtcat taatttcttc attaataggg attctatttt cgatttttat
      121 tccatatttc tctaattgat ttattttatc cgggttatta cttaaaagat ttatactttt
      181 tatgtttaaa ttttttaata tttgagcagc catatagaaa tctctcatat cttcttcaaa
      241 tccaagcatt aaatttgctt caacagtatc gtaaccttct tcttgaagtt tataagcctt
      301 aattttattt aaaaggccaa tacctctacc ttcttgcttc atataaatta aaactccact
      361 tccattttcc acaattttct tcatagaaga ttctagctgt aatccacaat cacattttag
      421 agagtggaac acatctcctg ttaaacattc agaatgaagt ctaactagag tattttctat
      481 atttatattt ccataagtaa gggcaatgtg ttcttcattt gaataagtat ctctatatcc
      541 tatgatttca aaatttccat attttgttgg aaggaaagca ctagatactt tttctatagt
      601 agtttcattt attttgcggt attctataag gtcttttata gtgatgattt ttaaattatg
      661 cttttctttg aaaatcatta aatcatcaac tcttgccatt gtaccatcat ctttcattat
      721 ttcacagata agaccaatct ctttaaaacc tgcaagtcta gcaaggtcta ctgaggcttc
      781 tgtatgtcca tccctgacaa gaactccacc agtttttgct attaaaggaa atatgtgacc
      841 aggcgttttg aaatctttac ttgtagaatt ttcatcacag aacaatttta cagtttcagc
      901 tctttcatat gcagatatac cagttagagt atctgctcca tctatagtaa cagtaaaggc
      961 tgtttgaaat gtatctgtat tttctctaac cataagaggt atattaagac ttttaaattt
     1021 tttttcagtg gcaggtagac aaattagacc tctaccatat gtagccataa aatttattga
     1081 ttcaggagta gccatttcag ctgccatcaa taaatctcct tcattctctc tactttcatc
     1141 atcaactacg attaccattt tcccattttt tatatcttca atagcttctt caatagtatt
     1201 aaacatgtaa ttcactcctt tattattttt aaaaaccatt tttaaataaa aaatctttat
     1261 ctatatttga tttatggtta ctactattag aatttaattc cttgtagtta ttagccataa
     1321 aattattaac atactttcca attacatcgc tttctaaatt aacaaaatct ccaacattct
     1381 ttgtcaagag aatagtgtta attttagtat gaggtataat agaaacctta aatatttcat
     1441 catctaaata tgatatagtt aaacttacac catcaatagc aacagaacct ttgagtatca
     1501 ttgaagataa aagtttttta tcaggtcgta tacttactaa aactgcattt tcatcttttt
     1561 caaatttgca gatagttcct ttaccatcaa catgacctgt tactatatga ccaccaaatc
     1621 ttgaagaaag actcatagct ctttcaagat ttaccttatc atttgcattt aataaaccta
     1681 aatttgtgga acgaattgtc tccatcataa catcggctgt aaattcattt tttccgaggt
     1741 ttgaaacagt tagacaaact ccatttacgg ctatactatc tccaagattt gtgccatcta
     1801 atattttatt tgatagtatg gttacttttc cactcttacc attagcagta atttttctaa
     1861 gtattccaac ttcttctact atgccagtaa acatcctaac ctcctatatg aatgtattaa
     1921 caaaaattta atttaaaaca tctgattcta taagaatatc gccatctatc aaagttacat
     1981 tattgattgt taactgtgtt gaatctgaca aatgatttat tccttctcca gaaacgaatg
     2041 taggtgcatc tttaccacct ataatcttgg gtgatagaaa taatttgact ttatcaacta
     2101 acttatttct aaagagactg tcattgagag tagcaccacc ttcaactaga acagaatcaa
     2161 tgtttaactc tcctaattta tacattacaa attccaaatc tactaagttg ttttttgaag
     2221 gagaaattat tgtttcaaca ttattttctt ttaacataga taattttata ctttctttgc
     2281 aacatgtgaa aactatagtt ttactggttt tatctttaac caaattacta ttaagtggca
     2341 actttaggtg tgtatctatt actattcttg taggatgaga cactttttct tgattgagcc
     2401 tgcaagttag taaaggatta tcgttcaaaa ctgtatttat gccaaccatt atactttggt
     2461 atttatttct gtatttatga gttagatacc ttgatgattc attagatatc cattttgatt
     2521 ctagtgattt tgtagcaatt ttaccatcta gacttactgc tgattttaca atacaaagag
     2581 gtcttttgtt ttttatatga taaaaaaatg cctcatttaa atctcttgct tcatcttcta
     2641 atattcccac agtaacatca atattgttat cttttaattt ttttactcca ttaccacaaa
     2701 caagtggatt tgggtctaat gtagatatta caactctctt aatcttattt tgtataattt
     2761 tatctacaca aggaggcgtt ttcccataat gagaacatgg ttctaagttt acataaagag
     2821 tagagtcttt tagactttgt ttagcactgt ttatagcatt tacttcagca tgattagagc
     2881 caaatttctc atgatatcct ttgcctatta tattactatc tttaacaatt acacacccaa
     2941 ctaatggatt tggatttaca aagccttctc catttttagc caattcaata gcttttttca
     3001 tataataaat atctttttct ttttgattca caatatgtac acctcctgtt taattggata
     3061 atttttaaac aaaaaaccct gagaattaac tcagggttta gaataatcta caataaatgt
     3121 ttaataaaag tagaagaaaa ttgctctaca aaaacactca ttaaatatct tcttccatcc
     3181 agactttact gtcggctttg gaatttaacc aaatcaacca agttttacct tggctcgcgg
     3241 gctataccgc cgattgggaa tttcacccta ccccgaagat taagtattaa atttatattt
     3301 agaatgtatc ataaattatt ttactttgca aactattttt ttaaaaaata tatgttaata
     3361 tataatatag aatgtctata aactttgaaa taccacgatt gtgaatataa aaataaaaat
     3421 ttgaacataa tcaggaaaga agtctaatta atttaaatta ggagagtaat atatgaataa
     3481 aaaactttta gaaatactat caaaatacta tggatataca agttttagaa aaggtcagga
     3541 atctataata aattctatct taagcgaaaa agatgtgtta gctattatgc caacaggagg
     3601 ggggaaatca atatgttatc aaattccagc tttgatttta gatggaatga caatagtaat
     3661 ttctccactt atatcactta tgaaagacca agtagatgcc ttaaagacta tgggaattag
     3721 tgctgctttt ataaatagtt cattaagcag caaagaattt aatgaaatat taaataatat
     3781 tagaaaaaat aattataaaa tactctatat tgctccagag agattagatg cacaagagtt
     3841 tattgagcta ataaataata acaatgtaag tcaagttgcg atagatgaag cacattgtgt
     3901 atctcaatgg gggcatgatt ttaggttgag ttatagaagg atttctgatt ttataaaaaa
     3961 tttgcctaaa agacctatag ttacagcatt tactgcaaca gcttcagaag aggtaagaac
     4021 tgatataata aatttactgt gtttagaaaa tccagattat tatataactg gatttgatag
     4081 agagaattta tcaataaata tagtaaaatc atctagtaaa aataagtata ttttagatta
     4141 tattcaaaat cataaaaatg agtctggaat tatatatgtt gcaactagaa aggaagttga
     4201 aaatatatat aatggtttat ctaagagaaa tatatctgta tcaaaatatc atgcaggttt
     4261 atctaaaaat gaaagaagta aaaatcaaga agattttata aatgataatg tggatataat
     4321 ggtagcaaca aatgcatttg gaatgggtat tgataaacct aatataagat gggttataca
     4381 ttacaatatg cctcaaagta tagaaaacta ctatcaagaa ataggtagag ctggtaggga
     4441 tggagaaaaa agtgagtgtg tacttttatt ttctccagga gatgtacata tacaaaaata
     4501 tttaattgat attggagttg aaaatcctga aagaaaatta tttcagcata agaagcttca
     4561 atacatgatt gacttagttt acagtaattc atgctataga aagacaatat tgagttattt
     4621 tggagaaaat tatatagaca attgcaataa ttgtagcaat tgtttagtag agggtgaaat
     4681 agtagataaa actatagatg cacaaaaagt catttcatgt gtagctagaa tgaaaagaag
     4741 ttatggtgtt actactattg tagatgtact tagaggttct aagaataaaa agattttaca
     4801 gcttggatta aatactttgt ctacatataa cattatgaga gattattcta gtgaagactt
     4861 aaagaatttt gtaaatacat tggtttctca tggtttttta gatttagttg agactctagg
     4921 caatggaaat agaggtagtt ttcctactat aagattaaat gagatgtcta tgaaagtttt
     4981 aaaaggtgaa gtgaaagtag agtttaaaga aattgttatg acaaaatcat tggaagtaga
     5041 ggatgagtta tataatgctt taagagaact tagacattca attgcaagtg aagaaaggat
     5101 agctccatat atggtatttg gagatggtac acttagagcc atgtctagta gttatccaat
     5161 taataaagaa gaaatgctag atatctctgg agtaggagaa attaaatatc aaaaatatgg
     5221 tagagatttt gaagcagtaa ttaaagaata tgtcgaaaaa aataatataa ataaaaaaat
     5281 agatgaaaaa aatgtcgatt taaagaacgt taatagtgat ttcttagagg ttaatacaga
     5341 taaagtttta tatgaaaaac tgttagtcat tagagaagaa tatgcaacaa aggaaaaatt
     5401 acctgcatat atgattttag ccaataaggc tttgaaagaa ataagtggaa gatatccact
     5461 tgatgaagaa caattaaaag atatttctgg cataggtgga gtaaaatttg aaaaatatgg
     5521 agctaatatt ttggcagagg ttaataaata tgtaaaagaa aataatatta atgttatttg
     5581 ggagaaaaag ggcaaaagga agttaatatt agatggagaa agtagaaaaa ataatgaaat
     5641 agcattagat ttgctaaatc aaggaaaaga tatacaaagt atcagtcaag aaatagaagt
     5701 ttcattgtca accattattg gatatgtgca tgactatgta aaggatggac atagtatatc
     5761 atttgaatta ggactagaaa atttttataa tgaagataaa aagaagatga ttttagaagt
     5821 ttgcgaaaaa gttggctatg ataatttaaa taacataaaa agaaaacttc cagattcaat
     5881 aaagtatgaa aatataagag ctgtgatgct tgactcttat cttgaaaact taaaaaataa
     5941 tattatagca gagtaatttt taacttaata aaaataaaat ttgtattatt gtgttgaact
     6001 aataattttg ctgtgttata ctgaatttgt aaaaataatg aaaaattaga gctaggggcg
     6061 ctagaattgg ctagctgaga gataaagccg taacttacaa accctaataa cctgatcaag
     6121 ataatgcttg cgaagggaag ctttttaata taattttatt atataaatag gcaaattatt
     6181 caatgttagg agaaatttgt ctaagtgtat atacgattat aagagtgctt tccaaacgag
     6241 gaaaagcact ttttttattt tcaaaaacta taactttaat cttggaggat tgtaatatga
     6301 actatacaac acaaatggat gctgctagaa aaggcataat aactaaagaa atggaaatcg
     6361 tttctcaaaa agaacaggta gatgtaaatg aattaagaga attaatagca aatggacaag
     6421 ttgttatacc agctaataaa aatcataaat ctttaagtgc agaaggtgta ggtaaaaatt
     6481 taagaacaaa aataaatgta aatctaggaa tttcaagaga ttgtaaggat atagaaaaag
     6541 aacttgaaaa agttagagta gcaatagaca tgaaagctga agctataatg gatttaagta
     6601 attatggcaa aactagagaa tttagagaaa aggttgttga aatgtcacct gcaatgatag
     6661 gttcagtacc tatgtatgat gcagtaggat atttggaaaa agaattaaaa gatataacag
     6721 aagaagagtt tttaaatgta ataagacaac atgctattga tggagtagac tttattacta
     6781 tacatgcagg tcttacaaga agtgtttgcc aaaaaataaa aaatcatgag agattgactc
     6841 atatagtatc tcgtggaggt tctttattgt ttgcatggat ggaattaaat aataaggaaa
     6901 atcctattta tactaatttt gataaaatat tggatatatg tgaagaatat gatgttacat
     6961 tgagtttagg agatgcttgt agaccaggtt gtataaaaga ttctactgat ggtgttcaaa
     7021 ttcaagaact tgtagtatta ggtgaactta ctaaaagagc atgggaaaga aatgtacaag
     7081 taatgataga aggtcctgga cacatggcta ttgatgaaat agaagcaaat gtagtattag
     7141 aaaagagatt atgccatgga gcaccttttt atgttcttgg accattggtt acagatatag
     7201 caccaggtta tgaccatata acaagtgcaa taggaggagc tttagcatgt gctaaaggag
     7261 tagatttttt atgttatgta actccagcag aacatttaag attgcctaat ttagatgata
     7321 tgaaagaggg tataatagct gcaaagatag ctgctcatgc aggagatata gctaaaaatg
     7381 ttaaaggtgc tcgtgaatgg gataataaga tgagtaaagc tagagctgat ttagactggt
     7441 gtgagatgtt tagacttgca atagaccctg aaaaagctaa gagatacaga gatgaatcaa
     7501 ctcctactca tgaggatagt tgtactatgt gtggaaaaat gtgctcaatg agaacagtta
     7561 agaaaatatt aaacaatgag gaacttaatc taatataatt tttggctagt atgactggaa
     7621 taaaaagaag ctgaaaagag gaggtgattt agtgaaggtt aatgggaagg aaattgaatt
     7681 tgaaaaagat ttgactgtta tagatttatt aaataaatat aatttaaaaa gtgatagagt
     7741 tgtagttgaa gtgaatttag agataattga agaatctaac tacaatacat atgtattaaa
     7801 agacgaggac attgtagagc ttattagctt tattggaggt ggttaaaata aaaatatttg
     7861 taaatgaatt attaacagat gttgaggaaa atacaactgc ttataaagtt agagatagct
     7921 ataacaataa atgtgatgta ttggtgttaa atggatatcc aattaaaaca gatatgcccc
     7981 tttgtgaaaa tgataaatta acacttatac aaaaaggagt taagccatca cttgatgaat
     8041 tggaaagctt gttgattgct agacatactc caaatataca taataaatta aaaaaaggga
     8101 aagttgctat attgggattg gggggacttg gttccaatat agctatctct ttagctagaa
     8161 taggagtggg agaactttta cttatagatt atgatgttgt agaaccatct aatttaaata
     8221 gacaacagta ttttatagat gatataggaa aactaaaaac tgatgccatg atagaaaata
     8281 taaaaaaaat aaatccattt ataaaattaa ataaacgaga tattttttta aacaagaaca
     8341 atatggatac tattaaggat tcagatttaa taattgaggc ctttgatgat gcttcatgta
     8401 aagcacaggt atgtaattat gtattaataa atttaaaaga taaatattta atagcatcat
     8461 ctggtatggc aggttactat gattcaaata taataactac taagaagata aaagacaggt
     8521 tttatatatg tggggatttt gtgaatgaag ctaaatttgg tgaagggctt atggctccaa
     8581 gggttgccat ctgcgcaaat catatggcaa atttggcaac gcaaatactt atagatatgt
     8641 aaatattaaa attagatata aaaaagattg aatattaaaa aaatttaacg tggagggaaa
     8701 taatatggat aaattggtac ttggtggaca tgaatttaat agcagattat tggttggaac
     8761 aggtaaatat ggttcaaata atatattgcc tgaggtaata aaagaaagtg gaagtgaaat
     8821 aataacaatg gcacttagaa gagtggattt agacaataaa caagaaaaca tattaacgta
     8881 tattccaaaa gagatgacaa tacttccaaa tacatcagga gctactaatg cagaagaagc
     8941 tgtaagaata gctagaatat ctagaaaaat gggatgtgga gattttataa aaatagaagt
     9001 aatatcagat actagatatt tacttcctga taatgaagaa actataaaag ctacaaagat
     9061 acttgctgat gaggggttta tagttcttcc atatatgaca ccagaccttt atgctggaag
     9121 aagacttata gaagctaatg cagccgcagt tatgccactt ggagctccaa ttggttcaaa
     9181 tagaggtctt caaatgaaag aaatgataag aataatgata gatgagttgg atataccaat
     9241 aatagtagat gcaggtattg gaaaaccatc tcaagctatg gaggctatgg aaatgggagc
     9301 tgatgcagta cttgtcaaca ctgctatagc ttcagcagga gaccctgtac aaatggctag
     9361 agcatttaaa ttagctgtag aaggtggaag agaagcatat attgcaaaaa caggaaatgt
     9421 gtcagaattt gcaaatgcat cttctccttt aacaggtttt ttaggcaatt tatagaaagt
     9481 ggtgattggt gatgagtttt tatgatgtaa tagaaaaata tagggatttt gattttgatg
     9541 gatatttaaa taatgttact gataatgatg ttttaagaag tttatcaaaa gacaagcttg
     9601 aagattttga tatattaaat ttgctttcta aaactgcagt aaaacattta gaagatatgg
     9661 cacaaaaagc acataaatta agtgttcaat actttggtaa aacagtatgt ttatatacac
     9721 caatgtatat agcaaattat tgtgttaatc aatgtgttta ttgtagctat aatataaaga
     9781 gtggaataaa aagaaaaaaa ttaactatgg atgaaattag agaagaggga gaagcaattt
     9841 caaaagaagg tttcaaacat ctacttgttt tgactggaga aagtagtttt cactcaagtg
     9901 tagaatatat aggtgaggct atagaaatat taagagagaa atttccctct ataggtatag
     9961 aagtttatcc aatggaagta gaagaatata agtacattgt agataaaggt gttgaaggat
    10021 taacagtata tcaagaaact tatgacgagg aaatatataa gagagtacat ataaaaggcc
    10081 caaaatcaaa ttatgaattt agactagatg ctcctgaaag aggggcaaaa gcaggtatga
    10141 ggactttatc aataggtgca cttttaggtc taaatgattt tagaaaagaa acttttttta
    10201 caatattgca tgggaaatac ttaaaaacga aatatcctca tattgagctt tcatattcaa
    10261 caccaagaat gagacctttt aaagggtgtt ttgaggaatt ggtagatata agtgatactg
    10321 atttagttca agctatggta tgtatgagat tatttgaccc tcatgcagct ataaatatat
    10381 ccactagaga aaatttagaa atgagaagtc atataatacc tttaggagta actaaattga
    10441 gcgcaggtgt atctactgat gtaggaggtc attctcaaga tgaacatgat actgctcaat
    10501 ttaagataaa tgatgaaagt actgttaaag atgtggagaa aatgctaaac tcaattggtt
    10561 atcagcatgt gtttaaagat tgggaaagat tttaaatgta tctgataaca aacagaaaat
    10621 tatgtagtga ggaacgatat ttagaagtta ttaaagagtc tatattgagt ggtgtagaaa
    10681 atataataat aagagaaaag gatttggaat atcaagagct aagaaaatta tatatgaaaa
    10741 taaaaactaa aatcaattgt atagattttc aagagcaaat atcagatgaa agtttgaaaa
    10801 ctaatataaa tcaaaaagag tgtagaaata aattcaaggt taattttata ataaatagta
    10861 acattgaatt ttttgaaaaa gtggattgtc aaggtatcca tcttcctttt aaattattct
    10921 tgaatttaat tgaaaataag tataatttta atgaaaataa aatactagga ttatctttac
    10981 ataaagtaga ggaagtagat tatttagaaa aattaataag aaatcaaaat ataaaaatag
    11041 attacataac attatcacat atatatgaaa ctaaatgtaa agaaggatta aatccaaaag
    11101 gaattgagct tttaaaagaa gctaaaaaga ttactgatat taaaataatt gcacttgggg
    11161 gaattttacc atctaatgtc aaagaaacat taaaatactg cgatgatttt gctataatgt
    11221 caactataat gaggtctaaa gatattaaaa aaaccatatc aaattataat gaaaagttga
    11281 attagtaaat atagtatatt gaatttttaa cttaaataaa aataaaaaag ttatctttta
    11341 aattttaaaa ggtagctttt ttattttata tctttgctaa taaaaaattg taaaaaggga
    11401 aaactttagt cagagagatt aaacaatatc ctagaaagat ttaaaattta gtaaagtatg
    11461 gaggacatta taatgactga cagtaaaaag aaacgattta ttataccaca cacttttaca
    11521 ataatatttt tactaatagt cttgatggca atattgactt ggatagtacc aagtggtgaa
    11581 tttagtagag aagatgtaga aggtagaatg gtagttgtac cagggactta tcaaagtgta
    11641 gaaagtaatc ctcaaggatt tgcagatgtt tttaaagcac caatagaagg ctttatagat
    11701 tcagctgaag ttgtaggttt tgttttaata gttggaggtg cttttggtat tgtaaatagg
    11761 actggagcta tagaagcagg aatagcttcg gttatctcta agtttaaggg aaaagaactt
    11821 cttataattc caatatcaat gattttattt ggacttggag gtacaacatt tgggatggca
    11881 gaagagacat taccatttta tatgatattt gttccactca tgacatcctt gggatatgat
    11941 tctctaacag caatagcaat tgtatttata ggagcagcag caggagccgc cgcttcaaca
    12001 ataaatccat tttcagttgg tattgcacag gcactagctc aacttgctcc tggttcagga
    12061 gtaacttaca gagtagttat ttttatagca tatatactaa taagtatagc atttgttatg
    12121 tactatgcta agaaggtaaa ggaaaatcct gaaagttcaa tagtatatga aatagataaa
    12181 aataataaat caatacttaa tcaaacttca acagaaataa aagaatttac ttttaaagaa
    12241 gtatctgttc ttgctatatt tggaataggg atggcaataa tgatttgggg tgttttaagt
    12301 caaggatggt acattccaga aatatcaatg tgttttgctg caataggtgt gctttctgga
    12361 ttggttggta gattatctca aattgaaatc tcagaaagtt ttatagatgg agcaaaagat
    12421 ttagcatctg cagctcttgc tatagctttg gctcgtggaa tcgtaataat tgctcaaaat
    12481 ggatttataa tagatactat attaaattcc gcagcagcct ttctaggtgg gcttccaaag
    12541 gttatatttg tatatttatc atttttcttg gaagctgcat tggcattttt aattccatca
    12601 tcttcaggtc ttgcttcact tacagtacct gtacttgctc ctcttggtga tttggttaat
    12661 atatcggctc aagtgatagt aaccgcttat caatttggtg tgggtcttac aaactttata
    12721 actccaactt caggagtact gatgggagca cttgcagtag cccatattcc attttcaaaa
    12781 tgggtaagat ttataatgcc tatcttgttg attttgacag ctgtatctct agtcttttta
    12841 acaataggta tttatatagg gttttaatta agtgtagttt tatttaagat atttagtaat
    12901 ggtataaaat gataatatgt tttcaataaa ttgccactaa tttaattagt ggtaatttat
    12961 tttttgtatt taaaaataaa tgtatttact tgtattttga attaatatct acttagacat
    13021 tgttttaatt gttatatttt catttaatta agcattgatt tatcgttttt tatatttcat
    13081 ataacaatgc aaaaaataat ttttatatag taaatgacaa aagtttacaa taaatgtata
    13141 ttttcttact cattccttaa aattactcat ttagtttaat aactctcatt tcatatgtaa
    13201 agttggtaaa aaaattaaaa ttaaaagtaa aaaactaaat aaattaatgt taatttgaaa
    13261 tatatatgaa ggtttttgga gaatacttac ttatgatagg aaaaaatgtt aaaataatag
    13321 tgtaatcaaa tataaaggac tgaaataatt aacagttatt aaatattagg agtgtaaaaa
    13381 tttatgggat taaaagaatt tgattttatt gaagagtcta ttgatatgtt gaggacaatg
    13441 tcaccaacat tagaaactat atcagatgaa atagaagagt attttgaaaa tatcttggat
    13501 gaaaagaatc aagaatatat aaatgtaacc tctagaataa aatcagaatc aagtttaaga
    13561 gaaaagataa ttagaaatag atatttaaaa aaatatggag aagcaagtaa tcttattcat
    13621 aatgtttctg acttaatagg cctaagaatt gagtgtagat ttattgagga tgaaaacaaa
    13681 atatatagac ttttgagaag atattttaat aaaacagatg acaaaataaa ctattataat
    13741 aaagagaata agaatattaa attaaaacta tctgagaggc aacccaataa gcaaaaaaat
    13801 ggatttgaaa tatataaaat agatggagtt tttgcgtatt tagatagaga agtaaaattt
    13861 gaacttcaaa ttaaatctct tgtaaatgtg ttttggagtg aaatagaaca taaaataatt
    13921 tataaaaata atacatattt acttgctgat aaatttataa aggatatgat ggactctata
    13981 aagaataatc ttactatgat agataatcag cttttaagta tttataaaaa ctttcattca
    14041 gggaagtctt ttaatatgaa agtaagtaaa aaagaaattg aaaaattatt tgcgaaacta
    14101 gtctatgacg ctttttctga aaagatgaat aaaagtatag gttttgttgt agattttaaa
    14161 aaaccatgtg aaactatatt gaactattct tttaataaac aagatataag tgatgaagtt
    14221 ttaggtaatt ttatgcttga tgagtttgca agattaaatg aaattgtaaa caaggatata
    14281 gattttaatg agcaaataga atttgaaaga gaaccaacat ttgatgataa gttttgtaaa
    14341 gacctaggaa atcattttag aagtagatta aacacagaat ttccatggaa cctatttttt
    14401 agaatattat ttgaaataga gccatataac aataccaaag actttgaaaa ctttgtagag
    14461 tttataaaag aaaatgtgct actagaagaa aatagggagc aattattatg ccaatttgaa
    14521 gaagatagca agcttataat tgaagatata tatgattgta tatttaatag tataagtgag
    14581 attgatagtg ttgaaatttt atataactat aatcttgaaa aaataaactt tacatcaagt
    14641 gaaatcataa attgtatatg tagagaatat gagtgttatg gtgaatactt agaagaaaaa
    14701 gaaaagctta tgaatacatt taaagaaaag attattcaaa tctttgagta ataggtcaat
    14761 gctgaagtgg cgctaatgcg ccatttttca aatatatatg aattaagtaa aattattttg
    14821 gaggaggcga tttgtataga gaaaacaggt gtaattttgg cagggggaaa aaattctaga
    14881 atgggtagag ataaagcctt tttagagtta catgataagc tttttattga aattgcaata
    14941 gaagcattta aaaactttga tgagttgata ataatatcta ataatgaaga attatattct
    15001 aaatacgata ttaaagttta taatgatata gtaaaagatg ttggtccaat tggaggaata
    15061 tatactgctc ttgaatatgc aaagtatgat atagttacaa ttgcatgtga tatgccatat
    15121 ctaaataatc aaatagtcga aagaatagct aataaaatga atgataaaag tgtgatttca
    15181 gtgacaaatg gaaagttaca accattgtgt tctggatata aaaagagtat aataaataaa
    15241 gtatctttat gtataaggga aaatgattta aagctcagaa gctttataga taaaatagat
    15301 aaaagttata tatactttga tgaagaagat ttatttttaa atgtaaacac tgttgatgag
    15361 tatgaaaaat taactaaagt ttaattagct atggttgtaa aatagagtag gatttatatg
    15421 gaggagagtt tatgaattta cttaaatgcg attcttgggc agtaatatta aataatagcg
    15481 ataaagaaag taaagcatat aaaatattag atgaacttaa aaggaatatg tacaaagttg
    15541 ttgctataga tgaagagaaa aaacctattg aaggaataga tgtgtatgaa tgtttaaaag
    15601 atgttccaca caatattgat gttgtagcta ttattgataa acaatctaag atggatgtga
    15661 ttttagaaga agtagagctt ttggatatac aaaatatgtg gtttgaaaaa ggaagcttta
    15721 gtgaaatgat gattaaaaag actaaagatt taaaattaaa tatagaatat aatttaagtt
    15781 tatatgatga gttgactaga taaaattata tttcttaata ataaaaatat aattagttaa
    15841 taaaacatat tattttatag tatagattgg ttaataaaag tacagattgg ttaatgaaac
    15901 attaaattac aatatttgtt aaaagaattg tgagttacat gatataatgt attaaatagg
    15961 atactaagga gttgatatat tatgttaata gtaactacag aaaaagtaga aggtaaaaag
    16021 atatcaaagg ttttaggatt agtgagagga agtacaataa gagcaaaaca tgttggaaaa
    16081 gatataggag caagttttaa gaatcttgtt ggaggagaac ttactggata taatgaaatg
    16141 ctcactgaag caagacaaat tgccataggt agaatggttg aagatgcaga agctaaaggt
    16201 gcaaacgcag taatagcatt tagactgtct tcagcttcag ttatgcaagg ggcagcagaa
    16261 atgcttgctt atggaacagc agttgtttta gaagatgata atagtattct tgaaaaataa
    16321 aatataaata gttaaaaaga ggcatctttt aaaaatttta tggatgcctt tttttatttg
    16381 aaaaaactaa tttgagaaga aattttaact attatgacaa taaaattagg gtgatattat
    16441 ataaataagt aaaattatat tatataatgt atggtataga ctttaaaaat tttatttgta
    16501 gagaaaaata tggacaatgt tataataatt atagaaatta aaagtactta cattcattat
    16561 gtatgtgaca tgtataatta gttttatatt taatacagtt ttgaagtaat atcttcattt
    16621 tgagaacgtt ggaataataa aaaatgtatg tattataggt gatataaata aaaaataaat
    16681 ataatatgtt atattgctga tagaaaagag aataaaagaa aataggtttt attaaaagag
    16741 atttttattt ataggactta atcaatgtat agaggggtga gaacatatgt ttaatgttgc
    16801 aattataaca cttagtgata aaggatatga aggaaaaaga gaagatataa caggaaaaaa
    16861 gttaactgaa tttgttgaaa atacaggagc ttataaagtt actgagtatg tacttataaa
    16921 agatgataag gaaatgctaa aggaaaatat tataagactt tgtaatagta ataaaataga
    16981 tttaattctt acaaatggag gtactggttt ttcaaaaaga gatataaccc cagaagcaac
    17041 taaggaagtg ttggaaaaag aaataccagg tctgagcgaa tatatgagaa tgaaatccac
    17101 agaaattact aaaaaggcca tactaagtag gggtgtgagt ggaataagaa ataattctat
    17161 aataataaat ttacctggaa gtcctaaagg tgcagttgaa aatctaagtt ttataattga
    17221 tgttttagac catggcatag aagttttaag aggagaagct acagaatgtg ccactaaaaa
    17281 aagtgaatag tatataatct agatagataa aaaatagaca aagaggtaga taaagtatga
    17341 ttgataagta tggaagaaaa atagactacc ttcgtatttc acttacagat aagtgtaatt
    17401 taaggtgtgt ctattgtatg ccaccagatg taaaatttga taaaaattat ataaatgaaa
    17461 atctaagctt tgaagactac aagtttatta taaaggctat ggctgaacaa ggtataacaa
    17521 aaattagatt tactggagga gaacctcttt tatatcctaa attaaatgag cttataaaat
    17581 ttacaaaaga agaatgtgga ataaataaca taggaatgac tactaatgct attgggcttt
    17641 ctgatagaat ttttgagctt gaaaaatgtg gcttaaataa agttaatata agtttagatt
    17701 ctttgaagga atataagtat aaatccgtaa caagaggagg aaacttaaaa gatgtcttaa
    17761 aatcgattga atcttgttta aacttaggta taaaagtaaa gttaaattgc gttgctataa
    17821 gtgggtttaa tgatgatgag cttaaagatt ttatgttttt aacagttaaa tcaccaattg
    17881 atgtaagatt tatagagctt atgccattag gtgaggctaa tagagtttat aagaagggtt
    17941 attttaatgt aaaagaaatg attgaaaaaa tatatggttt atataaagtc aaaaatgatg
    18001 aaaaaagtac agcagaatat tatatgttta aaggagctaa aggaagaata ggaattataa
    18061 ctccattaag ctgttcattt tgtaacactt gtaatcgtat tagacttact tctagtggtg
    18121 caataaaatt atgtctacat tcaaaagaag aaatagatat aaaaccattt ttacataaac
    18181 cattgttatt tagagaatct atgaaagata taataaaaca aaaaccagaa aaacatcatt
    18241 taatagaaaa taaatgtagt gatacaaata ggcgtatgta tgaaattgga ggataagtaa
    18301 tgttaacaca tataaatgaa aaagggtatg ccaagatggt ggacataagt gaaaaagatg
    18361 atactaaaag aacggccgta gctacagcta caattactct tagtaaggaa gcattagaaa
    18421 aagtaaaaaa tggtcagata aaaaaaggag acgtattgtc tgtagctcaa gttgcaggta
    18481 taatgggagc taaaaataca tcttcaaaca taccaatgtg ccatcagatt aaattagttg
    18541 gatgtgattt agaatttgat gtagatgatt atatgtctgc tattaagata tacgcaacct
    18601 gtaaatctct tgggaaaact ggagtagaaa tggaagcttt aaatgcatgt tctttagcag
    18661 cacttacaat ttatgatatg tgtaaagcag ttgataaaaa aatggttata agtaatatcc
    18721 atcttgtgga gaagactggt ggaaaatcag gagattttaa gttcgagtaa attaataata
    18781 tattgggtta aaatttggag gaaatatggc gaaggtagtg tcaataaata taagtgagaa
    18841 aaagggtgta attaaagttc ctgtaccatg tgcagagcta aaaataaatc atgggatagt
    18901 tggagatgca catgcaggaa actggcatcg tcagataagc ttacttggaa aagaaagcat
    18961 agataaaatg aaaatgaaag gctttgacca attaaaatat ggagattttg ctgaaaatgt
    19021 aactacagaa gggatagaag ttttttccct gcctgttggt acaaagttaa gaatagggca
    19081 atgtgaagtt gaagtaactc aaatagggaa aaaatgtcac aatggatgtg aaattaagaa
    19141 attgacaggt gactgtgtga tgccacgaga aggaattttt gtaaaggttc taaaagaagg
    19201 aattataaat gtgaatgatt caatagatgt aatctaatga agtcactatt aaaatataat
    19261 tataagtaaa t
//
LOCUS       DAMJWP010000055        18626 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.056, whole genome shotgun sequence.
ACCESSION   DAMJWP010000055 DAMJWP010000000
VERSION     DAMJWP010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18626
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.056"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..956
                     /locus_tag="PC700_001631"
     CDS             <1..956
                     /locus_tag="PC700_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HDF2580944.1"
                     /translation="SMKYEILEKEIIHKEELRLSNECYKIIVEQTDSVVFEWNIKENK
                     SFVSQAWTEKFGYNNACKNIFKEIKDKDLVHSEDKAIFEGFLESIKKKNIHSQAVYRL
                     KKSNGEYIWCRTSITSIYNDENELLRVVGVIVDVDSDIKKYEELRTRAESDSLTNIYN
                     KGTFEKLVEETIVMNTGDKKDALFIIDLDDFKEINDNFGHPFGDFVLKTFADKIQASF
                     GNKDLVGRIGGDEFVVYMQDYVTEVNLHKKAKELNRVLSDNYTDLSFSFDSSVSIGIA
                     RYPQDGTSFFELFKNADRALYSIKASGKNSYCLFEEELYVQ"
     gene            complement(1646..2476)
                     /locus_tag="PC700_001632"
     CDS             complement(1646..2476)
                     /locus_tag="PC700_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2580945.1"
                     /translation="MSQVSKNYFTTGEFAKICGINKKTLFHYDDIGLFSPELKKENGY
                     RYYSYHQLSIFGIISSLREVKMPLKEIKAYIDKRTPNLLIELLEKKTIDIKNEIEKLN
                     NIQALMESTISFTKNACNIDANTITLKEHEEEYLVKTPITYKEQFLGDEEENFLYECI
                     NFMDNYELSDYGTIGSIIKGEDIINKDFESYSYLFTKVNKEYKKYPVSIKPKGLYVTA
                     YHKGSYETIYKAYEKLLNFFNQNNLRIGDFVYEEYLLYDISVRDSNEYLTQISAEVKI
                     "
     gene            2549..4276
                     /locus_tag="PC700_001633"
     CDS             2549..4276
                     /locus_tag="PC700_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2580946.1"
                     /translation="MKKLIHFLKPYRVLIVVVLIFTFLQTLGTLYIPTLTANIVNNGV
                     VKGDIDYIVKTGLIMMIVAGITALSAVLVCKVSANLSSGFCRDIREAVFVKSQDLSIN
                     DFNNIGTASMITRSTSDITLIGQSVFMFIQLVLPAPIITVSGLFLAYSIDKAMTIIIV
                     VVMFLFMLSAFLVGKKLIKLFKMMQIKMDNMNRVLREVVTGVRVIRAFNREHFEKKRF
                     DRTAIDYSETAISINKVFAVLMPIVMLIMNLGIVSIIWFGGMRVSNGNMEIGHIMALI
                     EYCILILFYLIMGVMVFMYIPRAGACADRVNQILDIEPEIVDGNGRKDTVSERGHLVF
                     KNVTFSYAQSEEPVLNNITFEAKSGEVTAIIGSTGSGKSTIANIIPRFFEIQSGEISI
                     NGQDVKKIPQKELRDKIGFVPQKAFLFSGTIEENIRYGKEDASIEEIKHAASIAQADE
                     FISDMEDKYDSFVAQGGNNLSGGQKQRISIARALVRKPEVYVFDDSFSALDFKTDKRL
                     RKALKNEIKDSSAIIIAQRISTIMDANQIIVLNDGKIVGIGKHKDLLENCEVYKQIAD
                     SQLSKEELA"
     gene            4313..6175
                     /locus_tag="PC700_001634"
     CDS             4313..6175
                     /locus_tag="PC700_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2580947.1"
                     /translation="MSEKRIDEQDIEVLSFEDGMSAEKVKDAKKTTKRLLKYMAKQKF
                     KILIIFISVLISSALTVLAPMVMGKAIDQLFNGIKTAVQTGTKFSVNFSTMGGIVSIL
                     LGLYLISAVFIYIQQYIMSGVAQNLVLSMRKDLSDKLNKLPLKYYDSHKKGETLSIVT
                     NDLEKVADSLQEGLMQLITAVVTVVGSIVMMISISVPLTIVSAITLLVSLGITVVIAR
                     KSQERFSENQKALGELNSNIEEIFTGQIVVKAFSKEKDTIRNFKILNQNLYNASRRAQ
                     FSSYAISPIIRFINLIGYVIIAVVGGIFASTGAMTLGSIQAFIQYVNQASEPTTEISY
                     IVNMLQAAIASAERVFTVMDEVEEIKDEESSKVISMPKGKVQFEHVKFGYSDDFILMK
                     DININLNAGDKIAIVGPTGAGKTTLVNLLMRFYEIQGGRITIDGVNIKDLKRGELRTM
                     FGMVLQDTWLFNGSIKENIAYSKSDATMDEIVRAAKSARVDHFIRTLPQGYDTILTED
                     ASNLSQGQKQLLTIARAILSDPSVLILDEATSSVDTRTEVEIQKAMNNLMKGRTSFVI
                     AHRLSTIRDADLILVMKEGTIIEQGSHKELIEKKGFYEELYNSQFSSEYDEEVV"
     gene            6508..7197
                     /locus_tag="PC700_001635"
     CDS             6508..7197
                     /locus_tag="PC700_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HDF2580948.1"
                     /translation="MNKKLNYRFEMKHKITEADVLALKSRLYPIMKKDENASKDGKYL
                     IRSLYFDTPEDKALLDKLNGVAIREKFRIRFYNNNCSYIRLEKKIKHYNMTSKLSTNL
                     TKKEVINILNNDIEFLKESTNCLLREFYLKLKCERLEAKSIVDYNREAFIYPVGNVRV
                     TIDSDIKTSVNSIDLFNKDLPTVSVIDENMTVLEVKYDEFIPDFIKDLIQINKTTSTA
                     VSKYASSRLYI"
     gene            7302..7988
                     /locus_tag="PC700_001636"
     CDS             7302..7988
                     /locus_tag="PC700_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HDF2580949.1"
                     /translation="MILIMTFNDIFKSSFIENVSGFSFVDSALALGSAFLVGLFIYMV
                     YKKTYMGIMYSRPFNVSLVALTMLTTFVILAVTSNVVLSLGMVGALSIVRFRTAIKDP
                     MDLVFLFWSLGSGIVLGAGLIPLAVMGSIIMGLILIFFSNKTISETPYILMVNCNNED
                     SEDIATDKIKKVFTKYQIKSKSVTPEKGIELVFEVRMKYGETSLINDLSQVDGVTNAV
                     LVSYNGDYVA"
     gene            8005..9912
                     /locus_tag="PC700_001637"
     CDS             8005..9912
                     /locus_tag="PC700_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HDF2580950.1"
                     /translation="MKDKKFTLLISIMIIFLCAVVGVYSTSSNKSVDLYSDVYIEKYF
                     NRDKVMEVNIEIDESDLKDMNENAIKEEFKVAKVTVDGDTYGNVGIRTKGNSSLTSVA
                     NSDSDRYSYKINFDKYNTSQSMEGLTQLNLNNCYSDPSYMREFLTYSICEEMGLATPE
                     FAYAKVSINGEYHGLYLAVEGLKESYLENNFGNVTGDLYKSDEGSSLQYKGDDPESYS
                     NLIVESDKKTADWSKITKLLKSLDTGEDIEKYLDVDSVLKNIAINTALLNLDSYQGSF
                     AHNYYLYEQDGVFSMLPWDFNMSFGGFSGFGGGSQSIAIDEPTTGNLEDRPLISSLLK
                     NETHKTKYHKYLEEIVTKYLDSDYLENMTTKLHDMIASYVKEDPTAFYTYEEFEKNIT
                     SSIEDSSDNKGFGNKGFDNNNSNNSDSNNNSNSENKRSGNQSDKKEVNAELTSSVVKT
                     NTDNETENKTTNDSESKNNTDKDKSGNDNNQKLEGPRGKGGKSIPGVLEVAEDMSKTI
                     KSQLSGETSSTKQNSGDESSSGIKGSEKFDEDMSGMPEPPEGMDGKMPPGMGNMDKGD
                     MNGKNGNMNMDRNQDNPREAGGFGNRGGGSVSKTTTYFKLILGGASMIIMSIMLVGVS
                     RVKRRRFIKSK"
     gene            10365..11054
                     /locus_tag="PC700_001638"
     CDS             10365..11054
                     /locus_tag="PC700_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580951.1"
                     /translation="MLQKVLWKFLKLVVGLFICSVGIVLTINCNLGLSPWDVFHQGLS
                     NHIGITIGTASIIVGSIVVIADVVLGENVGWGTVFNMLLIGFFMDLLLYSNLIPEADS
                     LFVGIIMLILGLVLLSVGMVFYMGSGLGSGPRDGLMVAIQKKTGKSLKLIRGTIEVGA
                     LIVGFLLGGKVGIGTIISAFGLGYFTQMVFGLFKLDCSKIKHRFIVDDIKFIKVYMSG
                     EKKAAVQNIVK"
     gene            11490..12029
                     /locus_tag="PC700_001639"
     CDS             11490..12029
                     /locus_tag="PC700_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2580952.1"
                     /translation="MDIGEKIKRLRTEKQLTQEELANRCELSKGFISQLENNLTSPSI
                     ATLIDILEILGTNLREFFNEIDDERISFTKEDMFETEDEDLKYKLKWLIPNSQKNEME
                     PIIITLYPGGQYKEEKPHEGEEFGYVLAGSIYVHIGEKKNKVKKGESFYFRPKANHYI
                     SNEGKTTAKVIWVSTPPSF"
     gene            12048..13091
                     /locus_tag="PC700_001640"
     CDS             12048..13091
                     /locus_tag="PC700_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2580953.1"
                     /translation="MVENIIELRDISKHYEELTVLDNFNLDIKKNEFLTLLGPSGCGK
                     TTTLKIIAGFEYADDGKVLFEGKEINNLPPYERQVNTVFQKYALFPHMDVYENVAFGL
                     KIKKTPKDVIDAKVKEVLKLVALEGFERRQVESLSGGQQQRIAIARALVNEPKVLLLD
                     EPLGALDLKLRQEMQIELKRIQKKLGITFVFVTHDQEEALTMSDTIVVMYKGKIQQMG
                     TPQDIYNEPKNSFVAKFIGESNIFDGIMIEDYKVNFCNRDFECVDKGFEKNENIEVVI
                     RPEDIKMVKPEEGMLKGKVTSTVFKGVHYEIELNENGRLWILHNTKNAEVGTELGMDI
                     YPEDIHIMRKENN"
     gene            13093..13929
                     /locus_tag="PC700_001641"
     CDS             13093..13929
                     /locus_tag="PC700_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2580954.1"
                     /translation="MKRKSFLAYPYVVWSAIFVIIPLILVVFFSFTKESGGGYAFTLE
                     NYKEVIDPIYMKVFGRSILLAGGATLICLIVGYPVAYIISKARVSRRGSLILLFILPM
                     WMNFLLRTYAWVAILGKNGLLNTFLGWFGIQPLAILYTNFAILLGMVYNFLPFMVLPI
                     YTALSKMDNDLINAAHDLGANNMTVFRKIIFPLSLPGVMSGITMVFMPAVTTFAISRL
                     LGGGKIMLVGDLIEQQFTVVGDWNFGSAISIFMMIVILISMSIMSKFGDESDKEGGGL
                     LF"
     gene            14034..14708
                     /locus_tag="PC700_001642"
     CDS             14034..14708
                     /locus_tag="PC700_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2580955.1"
                     /translation="MARWNGFTWKWYGQLLQNESIMSALYYTIVIAILASVISTIVGT
                     ISAIGIHKMRGKSKKLILNVNYLPILNTEIVTAVALMSLFVFVKMEFGFTTMLLAHIM
                     FCLPYVILSVLPKMKQLPDNIEDAAMDLGATPIYALRKVILPQIKPGIVSGFLIAFTM
                     SIDDFIISFFNAGNGVSNLSIEIYGMARRGIKPEINALSTIMFAVVLGLLLLANKKES
                     IVRGIK"
     gene            14708..15760
                     /locus_tag="PC700_001643"
     CDS             14708..15760
                     /locus_tag="PC700_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2580956.1"
                     /translation="MIKFKKTISLIAVGMMTVTLFTGCNKATDSTKVLNVYNVGDYID
                     ESLIDKFEKETGIDVQYSTYDTNEMMYQKVKSGSTHYDLVFPSDYMVEKMKNEGLLEK
                     LDFKNIPNMKYLDKSFLNPVYDKTNEYSVAYMWGTLGILYNKKEVKDPMDSWNILWNP
                     KYKGNIMMFDSVRDTIGITLKKLGYSMNSVNPKEINEAKNLLMKQKDLVLAYVNDEGK
                     DRLLGGEVAMGMLYSGDAVTLMEQNPDLDYAIPKEGTNKWVDAMCVPTTAQNKKEAEL
                     FINFLLDPENAKVNAEYIGYSTPNTGALKLLDPEITQNPVAYPSKEVLDKCETFEDIG
                     ENIKLYDKAWIELKSK"
     gene            16204..17052
                     /locus_tag="PC700_001644"
     CDS             16204..17052
                     /locus_tag="PC700_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2580957.1"
                     /translation="MNRINKKIDLLILALLVFIFLIVGIVILSIRTENKLNVFIMLAI
                     IFFIIMLTYLSNSVVGLITSSIIIFMYTSYILYNNITHNMDVEFISYMWIIATPVSSI
                     IMGNLNKSINELQNTNKKLSEQYKELVTIDSETGLRNLKIFYNDVNMEISKSIRHNTD
                     FSLMIVKLPYYGNLQTIFGENKTNKIVKYIGSNIIECTRNEDIIYSLQKDMIGILMPN
                     TSLEGSKVVKDRIKKRIKELNLDLNNRGKYVNIDVKIAFLQYKSSFGDSINFKNIVEE
                     ELQYDV"
     gene            17042..>18626
                     /locus_tag="PC700_001645"
     CDS             17042..>18626
                     /locus_tag="PC700_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2580958.1"
                     /translation="MMFKIFNKSLLKLVIVLSIVLTISNFSLFSYASEITSSTDKVLI
                     IYDSKKETAYNRDILNIMRTLLGRFSSDIELLKLSNYDGEINENYYSHIFILGINENS
                     YNNDKNTKNLISSLNSYKVTICWLGYGIENLLEHKKYNLDYVGKTNNIVSVNYRGKSY
                     NLDEHYVFNIVESKDTSNKVIGSINDTLNKYPYIINDKNLFYVSKLDLDGVLFYIFCD
                     SLNDIFNIKTFDKGRIFVRIEDVHAFREPKNLVEIADYLSSKNIPFTIALIPAYVNPK
                     NHKVITLSESPEIVKAIKYMQDKGGTVILHGYTHQYKKEEVSGEGYEFWDGKKDEPLK
                     ENMKIFVKDRVLNGLRVCIENGIYPLAFEAPHYAMESEGYKELKKYFSTYMGQHQNND
                     KKFSTNTYPYIIRDTEEFNIFIPENLGYIDPEDKFTFQNIKENLDKLSIVRGFSGGFF
                     FHSYLNIEYLKNTIEYLEKQNIEFMNLRDFNNWVKVDEIQIRNNGDEIIVNYDKDLDE
                     ITKSDTRFKYISNISKILIF"
BASE COUNT         7287 a         1900 c         3232 g         6207 t
ORIGIN      
        1 tatctatgaa atatgaaata cttgaaaaag aaataattca taaggaagaa ttaagactta
       61 gtaatgagtg ttataaaatt attgttgaac agactgattc tgtcgttttc gaatggaata
      121 tcaaagaaaa taaatcattt gtatcgcagg cttggactga aaaatttgga tataacaatg
      181 catgtaagaa tatttttaaa gaaattaaag ataaagactt agttcattct gaagataaag
      241 ctatatttga aggttttctt gaaagtatta aaaaaaagaa tatacacagt caagcagtat
      301 atcgtttaaa aaaatctaat ggtgaatata tttggtgtag aacatcaatt acaagcattt
      361 ataatgatga aaatgaactt ttaagagttg taggagtaat tgtagacgta gatagtgata
      421 taaaaaaata tgaagagtta agaactagag cagaaagtga ctcattgacc aatatataca
      481 ataaaggtac atttgaaaaa ttagtggaag aaactatagt tatgaataca ggtgataaaa
      541 aagatgcatt attcattatt gatttagatg attttaaaga aataaatgat aattttggtc
      601 atccatttgg tgattttgta ctaaaaacat ttgctgataa gatacaagct agttttggta
      661 ataaagactt agtcggaaga attggtggag atgaatttgt tgtatatatg caagattatg
      721 ttacagaagt aaatttgcat aaaaaagcta aagaattaaa tagagtactt tcggataatt
      781 acactgattt gagttttagt tttgattctt ctgtcagtat aggtattgca aggtatcctc
      841 aagatggaac ttcatttttt gaattgttta aaaatgcaga tagagcactt tatagcataa
      901 aagcatcagg taaaaattca tattgtttat ttgaagaaga actatatgta cagtgaattt
      961 gaatttagat tttatgtgag agtaagattt atatatactt gtggctgtct gatttataaa
     1021 atatgttact ttgtatattt ttatatgttt cagtaaggtt aattaacata agcaaaggtg
     1081 taagtaaatt tatattagtt taagtttact tacaccttta taaaaataaa ttttgtaaat
     1141 ttaaacagtt ggttttataa ggatttttat ttattttaat atcttagata ttcctttttg
     1201 tattccttag agaaaataaa taatgaaagg agtacagaaa aaattaaaca tcctaatata
     1261 ccacttgaat taagtcttaa agcattaaag tttaaaaaag cattatttaa atcggtatcc
     1321 aaaagaaaag gatgctttga tttgctatat atggaaaaat taaaaatata ttcataatca
     1381 aatttataaa attaatatct ttccatccta acattacatt tacttagatt aattattatt
     1441 ataaaaataa aaatacaaat aatagattca tttacattct taaatgatta aagctcttta
     1501 ccctttaaac atgcataaca tatcgttata attccagtaa ataagactaa aatgcccatt
     1561 cctaaaattg cagtttcatc atctattact ccaaatgctt ttaaagtaac ttatttcgct
     1621 ttgtataata ataatttgtc tctttttata ttttaacttc tgctgagatt tgagttagat
     1681 attcgttaga atctcgcaca cttatatcat atagaagata ttcttcataa acaaaatccc
     1741 caatacgtaa attattctga ttaaaaaaat ttaataactt ttcataagcc ttgtaaatag
     1801 tttcataact acctttatga taagcagtta cataaagtcc ctttggtttg attgatacag
     1861 gatatttctt gtactcttta ttaacttttg taaataaata tgaatatgat tcaaagtctt
     1921 tattaattat atcttcacct ttaatgattg agccaatcgt accatagtca ctcaattcat
     1981 agttgtccat aaagttaata cattcatata aaaaattttc ttcctcatct cctaaaaact
     2041 gttctttata tgtaataggt gttttgacca agtactcttc ctcatgttct tttaaagtaa
     2101 tagtattcgc atctatatta caggcatttt tagtgaatga aatagtagat tccattagag
     2161 cttgaatatt atttagtttt tcaatttcat ttttaatatc aatggttttc ttttctaaaa
     2221 gctcaattag taaattaggt gttcttttgt ctatataagc tttaatttct tttaaaggca
     2281 tttttacttc cctaagtgaa gaaattatac caaaaatgct aagttgatgg tatgagtagt
     2341 atctatatcc attttctttc tttaattctg gtgagaaaag acctatatca tcataatgga
     2401 aaagtgtttt tttattgatt ccacaaatct ttgcaaattc tccagttgta aaataatttt
     2461 ttgaaacttg actcataatt ataattcctc cttgactatc ctgttacagt atagtttatc
     2521 atagtatttt gtaaaggagt gtgattatat gaaaaaatta attcattttt taaaaccata
     2581 tagagtttta attgttgtag ttttgatatt tacgttttta caaacacttg gaactttgta
     2641 tataccaaca cttacagcaa atatagtaaa caatggtgta gtaaagggag atatagatta
     2701 tattgtaaaa acaggtttaa taatgatgat agtagctggt ataacagctt tatcagctgt
     2761 gttagtttgt aaagtatcag caaatctttc atctggattt tgtagagata ttagagaagc
     2821 agtatttgta aaatcacaag atttatcaat aaatgatttt aataatatag gtacagcatc
     2881 aatgattact aggagtacta gtgatattac tttaataggt cagtctgtgt ttatgtttat
     2941 tcaattggta ttaccagcac caattataac tgtttctggt ctattcttag catattctat
     3001 agataaagca atgacaatta ttattgttgt agtaatgttt ttattcatgc tttcagcatt
     3061 tttagttggt aaaaagctta taaagttgtt taaaatgatg caaataaaga tggacaatat
     3121 gaatagggta ttacgtgagg tagtaactgg agtaagagtt ataagagctt ttaataggga
     3181 gcattttgaa aaaaagagat ttgatagaac tgcaatagat tattctgaaa cagcaatttc
     3241 aataaataaa gtatttgcag tacttatgcc tattgtaatg ttaataatga atttaggtat
     3301 agtaagtatt atttggtttg gtgggatgag agtcagtaat ggaaatatgg agataggtca
     3361 tataatggca cttattgaat attgtatctt aattttattc tatttaatca tgggagtaat
     3421 ggtatttatg tacataccta gagcaggtgc atgtgcagat agagtaaatc agatattaga
     3481 tatagaacct gaaattgtag atggaaatgg tcgtaaagat actgtctcag agaggggaca
     3541 tttagtattt aaaaatgtaa cattttcata tgcacaatca gaagaacctg tgttaaataa
     3601 tataacattt gaagcaaaat ctggagaagt aactgcaatt ataggaagca ctggctcagg
     3661 caaatctact attgcaaata taataccacg tttttttgaa attcaatctg gagaaatatc
     3721 tataaatggt caggatgtta agaagattcc tcaaaaagaa cttagagata agattggatt
     3781 tgtgccacaa aaagcttttt tatttagtgg aacaatagaa gaaaatataa ggtatggaaa
     3841 agaagatgca agtattgaag aaataaagca tgctgcaagt atagctcaag ctgatgaatt
     3901 tatttctgat atggaagaca agtatgactc atttgtagca caaggtggaa ataatttgtc
     3961 aggtggtcaa aaacagagaa tatccattgc aagagcgtta gttagaaagc ctgaagtata
     4021 tgtctttgat gatagttttt ctgcattaga ttttaaaaca gataaaagac ttagaaaagc
     4081 gttgaaaaat gaaatcaaag attcatcagc tataattata gcacaacgta taagtacaat
     4141 catggatgct aatcagatta tagttttaaa tgatggaaaa attgtaggta ttggaaaaca
     4201 taaagattta cttgaaaatt gtgaagttta taagcaaatt gcggattcac aacttagtaa
     4261 agaagaactt gcataaaatg aagaattaat ctgatatttg gaggtgtatg atatgagtga
     4321 aaaaagaata gatgaacaag atatagaagt acttagtttt gaagatggca tgtcagcaga
     4381 aaaagttaag gatgcaaaaa aaactactaa gagattatta aaatatatgg caaaacaaaa
     4441 gtttaaaata ttaattattt ttatttcagt attgataagt tcagcgctta cagttttagc
     4501 acctatggtt atgggaaaag ctattgacca actatttaat ggaataaaaa ctgcagtcca
     4561 aacaggtaca aaattcagtg tgaattttag tactatgggg gggatagtat caatacttct
     4621 agggttgtac ttaattagtg cagtatttat atatattcaa cagtatatta tgtcaggagt
     4681 tgcacaaaat ctagttctta gcatgagaaa agatttaagt gataaactca ataaacttcc
     4741 tcttaagtac tatgattctc ataaaaaggg ggaaacactt agtatagtaa caaatgattt
     4801 agaaaaagta gcagatagtt tacaagaagg attaatgcaa cttattacag cagtagttac
     4861 agtagttggt tctattgtaa tgatgataag tataagtgta ccacttacaa tagtatcagc
     4921 tataacatta cttgtttcat tgggtattac tgttgtaatt gcaagaaaat cacaagaaag
     4981 attctctgaa aatcaaaagg cactaggaga gttaaatagc aatatagaag agatatttac
     5041 tggtcaaata gtggttaaag catttagtaa agaaaaggat actataagaa actttaaaat
     5101 attaaatcaa aatctttata atgcaagtag aagagcacag ttttcgtcat atgcaatatc
     5161 tccaattatt agatttataa acctaattgg ctatgttatt atagcagttg taggtgggat
     5221 atttgcatca actggagcaa tgactcttgg tagtatacaa gcgtttattc aatatgtaaa
     5281 tcaagcatca gagcctacta cagaaatatc ttatattgta aatatgcttc aagcagcaat
     5341 agcatctgca gagagagttt ttacagttat ggatgaggtg gaagagataa aggatgaaga
     5401 atcttcaaag gttattagca tgcctaaagg taaagtacaa tttgagcatg ttaaatttgg
     5461 atatagtgat gattttattc ttatgaaaga tattaatatc aatctaaatg caggagacaa
     5521 gattgcaatt gttggaccta caggagctgg aaagacaact cttgtaaatt tacttatgag
     5581 attttatgag atacaaggtg gaagaattac tattgatggt gttaatataa aagacttaaa
     5641 gcgtggagaa cttagaacta tgtttggtat ggttcttcaa gatacatggc tgtttaatgg
     5701 aagtataaaa gaaaatattg catatagtaa gtcagatgcc acaatggatg aaattgtaag
     5761 agcagcaaaa tcagcaagag tagaccattt tataaggact ttaccacaag ggtatgacac
     5821 tatattgact gaggatgctt ctaatctatc tcaaggtcaa aagcaacttc taacaattgc
     5881 cagggcaata ctttcagacc catcagtatt gattcttgat gaagcaacat ctagtgtaga
     5941 tactagaaca gaggtagaaa ttcaaaaggc aatgaataac ttgatgaagg gaagaactag
     6001 ttttgttatt gcacatagat tatcaactat tcgagatgct gatttaatat tagtaatgaa
     6061 agaaggcact attattgaac aaggaagcca taaggaactt attgaaaaga aaggattcta
     6121 tgaagaactt tataatagtc aattttcaag tgaatatgat gaagaagttg tatgattatc
     6181 aagttttagg caactaaatg attgataaat attaagttaa atatagtaac atgtaaggtt
     6241 atatggaata agagtatttc tctatttcat ataacctttt ttatttatca aaataagata
     6301 aaattttctt tctaaattag attgaagaaa gtatttatca atataaaagt aaaataatca
     6361 ttattattag tgaagtaaat attgtttgcc acatatatgc cataaagatg cacttatttt
     6421 acggagattt aacaaagtag tgtaaagtat actctaaata taaaataaat caaatagagt
     6481 taaataaatt gagaggtgat ataaatagtg aataaaaaat tgaactatag atttgaaatg
     6541 aagcacaaaa taactgaagc agatgtgtta gctttaaaat caagactcta tccaataatg
     6601 aagaaagatg aaaatgcatc taaagatgga aaatatctta ttaggagttt atattttgat
     6661 actcctgaag ataaagcact tttagataaa ttaaatggcg ttgcaataag agaaaagttt
     6721 aggataagat tttataataa taactgctct tatataaggt tagagaaaaa aatcaaacat
     6781 tacaacatga catcaaagct atcaactaat ttaaccaaaa aagaagtaat aaatatacta
     6841 aacaatgata ttgaatttct aaaagagtcg acaaattgtt tattaagaga attttatttg
     6901 aaattaaaat gtgaaagatt agaggcaaaa agcatagttg attacaatag agaagctttt
     6961 atttatccag ttggaaatgt aagagttaca atcgatagtg atattaaaac ttctgtaaat
     7021 tcgatagatt tatttaataa agatttgccg actgtttcgg taattgatga gaatatgact
     7081 gtattggagg ttaaatatga tgaatttata ccagatttta taaaggattt aattcaaatt
     7141 aataagacta cttctacagc agtttcaaag tatgcttcaa gtagattata tatttaagaa
     7201 taaaggctat gatttatata aagatataca taaataaggt taaaaaacaa ataggttaaa
     7261 aataaataaa ataaaaaata aataagttaa aaataggaag gatgatttta attatgacat
     7321 ttaatgatat ttttaaatca agttttatag aaaacgtatc aggtttttca tttgtggaca
     7381 gtgctttagc actaggttca gcttttttag taggtttgtt tatatatatg gtctataaaa
     7441 agacttatat gggaattatg tattctcgac cattcaatgt gtcgcttgta gcacttacaa
     7501 tgcttacaac ttttgtaata ttggcggtta cttcaaatgt agttttatcc ttaggtatgg
     7561 ttggtgcttt atctatagta agatttagaa cagcaattaa agacccaatg gatttagtat
     7621 ttctattttg gtcattgggt tcaggtatag ttttaggagc aggtttgatt cccttagctg
     7681 tcatgggttc aataattatg ggattgattc taatattctt ttcaaataaa acaatatctg
     7741 aaactccata tatattgatg gtaaattgta ataatgaaga ttcagaagat atagcaactg
     7801 ataaaattaa aaaagtattt actaaatatc aaataaaatc aaaaagtgtt acaccagaaa
     7861 aaggaataga attagtgttt gaagtaagaa tgaaatatgg agagacaagt cttataaatg
     7921 acttgagtca agtagatgga gttactaatg cagttcttgt aagctataat ggtgactatg
     7981 tagcatagag aggagtaatc ttttatgaaa gataaaaaat ttacccttct tatctctatt
     8041 atgattatat ttttatgtgc tgtagttgga gtttatagta catctagcaa caaaagtgtt
     8101 gatttatata gtgatgtata tattgaaaaa tattttaaca gagacaaggt tatggaagtt
     8161 aatatagaga tagatgaaag tgacttgaag gatatgaatg aaaatgctat aaaagaagaa
     8221 tttaaggttg caaaagtaac tgtagatgga gatacatatg gaaacgtagg tataagaact
     8281 aaaggaaatt caagtcttac atctgtagca aatagtgata gtgatagata cagctataag
     8341 attaattttg ataagtataa tactagtcaa agtatggaag ggcttactca attaaatctt
     8401 aataactgtt actctgaccc atcttatatg agagagtttt taacatatag tatttgcgag
     8461 gaaatgggat tagcgactcc agaatttgca tatgctaaag tctctataaa tggcgaatat
     8521 catggtttgt atttggcagt agaaggatta aaagagtctt atcttgaaaa taattttggt
     8581 aatgtaactg gagacttata taagtcagat gaaggaagct cgttgcaata taaaggagat
     8641 gacccagaaa gttactcaaa cttaatcgtt gaaagtgata aaaagacagc tgattggtct
     8701 aaaattacaa aactattaaa atctttggat acaggtgaag atattgaaaa atatcttgat
     8761 gtagattctg tccttaaaaa tatagcaata aatacagctt tattaaacct tgatagctat
     8821 caaggcagtt ttgcccataa ctattattta tatgagcaag atggagtatt ttctatgtta
     8881 ccatgggatt ttaatatgtc atttggtgga tttagtggtt ttggtggagg tagtcaatct
     8941 atagcaattg atgaacctac gacaggtaat ttagaagaca gacctctcat atcctcgtta
     9001 ttaaaaaatg agacacacaa aacaaaatac cataaatatc tggaagagat agtaacaaaa
     9061 tacctagatt cagactattt agagaatatg acaacaaaat tgcatgacat gatagcatca
     9121 tatgtaaaag aagacccaac agcattttat acttatgaag aatttgaaaa aaatataaca
     9181 tcttcaattg aagattctag tgataataag ggatttggta ataaagggtt tgacaacaat
     9241 aactctaata acagtgattc taataataat tctaatagtg aaaataagcg ctctggaaat
     9301 caaagtgata aaaaagaagt taatgctgaa ttaacatcaa gcgtagtcaa aactaataca
     9361 gataatgaaa ctgaaaataa aactacaaat gatagcgaaa gtaagaataa tacagataaa
     9421 gataaaagtg gaaatgataa taatcaaaag ctagaaggtc ctaggggtaa aggaggtaag
     9481 tcaataccag gggttttgga agttgcagaa gatatgagta aaactataaa atctcaatta
     9541 agtggagaaa cttcttcgac aaagcaaaac tctggtgatg aaagttcaag tggaattaaa
     9601 ggtagtgaaa agtttgatga ggatatgagt ggtatgccag aaccacctga gggaatggat
     9661 ggtaaaatgc caccaggaat gggtaatatg gataagggag atatgaatgg taaaaatggc
     9721 aatatgaata tggatagaaa tcaagataat ccaagagaag ctggaggttt tggcaataga
     9781 ggaggaggct ctgtgagtaa aacaacaaca tacttcaaat taattttagg tggagcttca
     9841 atgataataa tgtcgattat gttagtaggt gtatcaaggg taaagagaag aagatttata
     9901 aagtcaaaat aagattaaaa ttgaatataa ataattttaa tactgttaag tctaaatatt
     9961 tacttagcag tattttttta gaaaataatg tgtttgatta ataatataag gtaataaatt
    10021 gataaaaatt aaatacagca tattaataat acataataaa gtaataaaaa aactgtttaa
    10081 gtataataac ttatatttag acagtttttt tcatttataa ataatataaa tgaaattgaa
    10141 aacatttgaa ggatagaaat tggttggata aatgtataaa ttacttcacc aaaaaataat
    10201 gacaagagtt gtaactaagt tgtaataaaa tggtagttat ttagcataaa ataatgtatc
    10261 atagaagtat attataaaat taataaaata agatttttgt aagaaaactg ctggcttttg
    10321 atatttagag tgagtagtta aattatgttt aggaggggta gaatttgttg caaaaagtat
    10381 tatggaaatt tttaaagcta gtagtcggtt tatttatatg ttcagttggt atagtattga
    10441 cgataaattg taaccttgga ttatctccct gggatgtatt tcatcaagga ctttcaaatc
    10501 atataggaat aactatagga actgcttcta taattgtagg ctctatagta gttattgctg
    10561 atgtcgttct tggagaaaat gtaggatggg gaactgtttt taacatgcta ctaattggat
    10621 tctttatgga tttactttta tatagtaatt taattccaga ggctgatagt ttatttgtag
    10681 gaataattat gttgatatta ggattggttc ttttgtctgt tggaatggta ttttatatgg
    10741 gaagtggttt aggaagtgga cctagagatg gtttgatggt tgcaattcaa aagaaaacag
    10801 gaaaatcact taaattaata agaggtacta tagaagttgg agcattaata gtaggatttt
    10861 tattaggtgg aaaagttggt ataggaacta taataagtgc atttggattg gggtatttca
    10921 cacaaatggt atttggctta tttaaattag attgtagtaa gattaaacat agatttatag
    10981 tagatgatat aaaatttatc aaagtgtata tgagtggtga gaaaaaagct gctgttcaaa
    11041 atatagttaa gtaatttaag aataaattca acgaaatgtt aagtatttta aattaacatt
    11101 ttatttagat ggataaattg aatattaaaa gaatattaaa atatgaggat tgctagaaca
    11161 ctataaaata tagatttcta gcaatttttt atttgttcaa tgttttttta gtactagaag
    11221 tcttattctc tatttaatga atgaattaat ttataattag agatactaaa ctttatagat
    11281 attaatataa acacctttaa actgtgtatt ttccaaagcc tttaaaatgc ttgtatttat
    11341 tctaaataaa aaaataaaaa aatgtcaaaa aaactcttgt attatatcca aattttatat
    11401 ataattacct atgttgttta gaaattttaa actcaatgtt tagtaaacaa aatgtttagt
    11461 aaaataaaac agaaactagg aggtgcaaca tggatatagg tgaaaagata aaaagattaa
    11521 gaacagaaaa acaactgacc caggaagagc tagctaatag atgtgaattg tcaaagggat
    11581 ttatttctca attagaaaat aacttaacat caccatccat agctaccctt atagatatac
    11641 ttgaaatact aggtacaaat ttaagggaat tttttaatga aatagatgat gaaagaatca
    11701 gttttacaaa agaagatatg tttgaaactg aagatgaaga tttaaaatat aaattaaagt
    11761 ggttaattcc aaattcacaa aaaaatgaaa tggagccaat aataataaca ttgtatccag
    11821 gaggacagta taaagaagaa aagccacatg aaggtgaaga atttggatat gtattagcag
    11881 gttcaatata tgtccatata ggcgaaaaga aaaataaagt aaaaaaagga gagagttttt
    11941 actttagacc aaaagcaaat cactatatat caaacgaagg caaaactaca gcaaaagtga
    12001 tttgggtaag tacgccacca tcattttaga atagaggtgt ttaataattg gtagaaaata
    12061 taatagaatt aagggatata tctaaacatt atgaggaact tactgtttta gataatttca
    12121 atttagatat aaagaaaaat gagtttttaa cattacttgg accaagtgga tgtggaaaaa
    12181 ctacaacatt aaaaataata gcaggatttg agtatgctga tgatggtaaa gttttatttg
    12241 aaggtaagga gataaataac ttgccaccat atgaaagaca agtaaacaca gtatttcaaa
    12301 aatatgcttt atttccacac atggatgttt atgaaaatgt agcttttgga ttaaaaatta
    12361 aaaagactcc aaaagatgta atagatgcta aagtaaaaga ggttttgaaa cttgttgcac
    12421 tagaaggttt tgaaagacga caagttgaat cattgagtgg aggacaacaa cagagaattg
    12481 caatagcaag agctctagtc aatgaaccaa aggtattatt actagatgaa ccacttggag
    12541 cacttgattt aaagttaaga caagagatgc aaatagaatt aaagagaatc caaaaaaaat
    12601 taggaataac atttgttttt gtaactcatg accaagaaga ggctcttact atgtcagata
    12661 ctattgttgt aatgtataag ggcaaaattc aacaaatggg aacaccacaa gatatataca
    12721 atgaacctaa aaattcattt gtagctaaat ttataggtga aagtaacata tttgatggaa
    12781 ttatgataga agattataaa gttaatttct gtaatagaga ttttgagtgt gttgataaag
    12841 gattcgaaaa aaatgaaaat atagaagtag taataagacc agaagatata aaaatggtaa
    12901 agccagaaga aggaatgtta aaggggaaag ttacatctac agtgtttaaa ggagtgcatt
    12961 acgagataga attgaatgaa aatggaagat tgtggatact tcataatacc aaaaatgctg
    13021 aggttgggac tgaactaggc atggatatat atccagaaga tatccatata atgagaaaag
    13081 agaataacta agatgaaaag aaaatctttt ttagcatatc catatgtagt ttggagtgcg
    13141 atatttgtaa taattccttt aatattagtg gtatttttta gttttacaaa agaatcaggt
    13201 ggaggttatg cctttacact tgagaattat aaagaagtaa ttgaccctat atatatgaaa
    13261 gtttttgggc gttctattct tttagcagga ggagctacat taatatgcct tatagttggt
    13321 tatccagtgg catatataat ttcaaaggct cgtgtaagta gaagaggttc attaatatta
    13381 ttgtttattc ttcctatgtg gatgaacttt cttttaagaa catatgcatg ggttgctata
    13441 ttaggaaaaa atggactttt aaatacattt ttaggttggt ttggaataca accattagca
    13501 attttgtata ctaattttgc tatactttta ggtatggtgt ataacttttt accatttatg
    13561 gttcttccaa tatatacagc actatcaaag atggataatg atttgataaa cgctgctcat
    13621 gacttgggtg caaataatat gacagttttt agaaaaatta tatttccttt gagtttgcca
    13681 ggtgtaatgt ctggaataac tatggtattt atgccagcag tcactacttt tgcaatatca
    13741 agactgttag gtggaggtaa aataatgctg gttggagatt tgattgaaca acagtttaca
    13801 gttgttggag attggaactt tggttcagct atatcgatat ttatgatgat agtaatatta
    13861 atatctatgt ctataatgtc aaaatttgga gatgaatctg acaaagaagg cggtgggtta
    13921 ctattttaaa attaaagaaa ataacttcta atatatattt agcattagta ttcttatttt
    13981 tgtatgcacc catatttgct ttggtactat tttctttcaa tgattcaaaa tcaatggcaa
    14041 gatggaatgg atttacatgg aagtggtatg gtcaattact tcaaaatgag agtataatga
    14101 gtgctttata ttatactatt gtaatagcta tattagcgtc tgtaatatct acaatagtag
    14161 gaactataag tgctatagga atacataaga tgagaggtaa gagtaagaaa ttaatactaa
    14221 atgtaaacta tcttcctatt ttaaatacag agatagttac agcagtagct cttatgagcc
    14281 tgtttgtatt tgtaaaaatg gaatttggat ttacaacaat gctattggca catataatgt
    14341 tttgtttgcc atacgttata ctttcagttt tgccaaaaat gaagcaatta cctgataata
    14401 tagaagatgc agctatggac ttgggagcaa cacctatata cgctcttaga aaagtaattt
    14461 taccacaaat aaaaccaggt atagtttctg gatttttaat tgcatttact atgtcaatag
    14521 atgactttat aataagtttc tttaatgctg gaaatggtgt aagcaatctt tctatagaaa
    14581 tctatggaat ggctagaaga ggtataaaac ctgaaataaa tgcattatca acaataatgt
    14641 ttgcagtggt tctaggttta cttttactag ctaataaaaa agaatctata gtaaggggga
    14701 ttaaataatg attaagttta aaaaaacaat atcccttata gctgtaggta tgatgacagt
    14761 tactttattt acaggatgta ataaagcaac tgattctaca aaagttttaa atgtatataa
    14821 tgttggagat tatatagatg agtctcttat agataagttt gaaaaagaaa caggaataga
    14881 tgttcaatat tcaacttatg atactaatga aatgatgtat caaaaagtaa agagtggtag
    14941 tacacactat gatttagtat ttccttcaga ctatatggta gagaaaatga aaaatgaagg
    15001 tcttttggaa aaattagatt ttaagaatat acctaatatg aaatatttag ataagagttt
    15061 tttaaatcct gtatacgaca aaactaatga atatagtgtt gcatatatgt ggggaactct
    15121 tggaatattg tacaataaga aagaagttaa agaccctatg gatagctgga atatactttg
    15181 gaatccaaag tataagggaa atataatgat gtttgactct gttagagata ctatagggat
    15241 aactcttaaa aaattaggat atagcatgaa tagtgtaaat ccaaaagaaa taaatgaagc
    15301 taaaaatctt ttaatgaaac aaaaggattt agtattggct tatgtaaatg atgaaggtaa
    15361 ggatagattg cttggtggag aagtggcaat gggtatgctg tattctggag atgcagttac
    15421 gctaatggag caaaatcctg accttgatta tgctatacct aaagagggaa ctaataaatg
    15481 ggtagatgct atgtgtgtac ctacaacagc acaaaataag aaagaagctg agctgtttat
    15541 aaacttttta ttagacccag agaatgcaaa agtaaatgct gagtacattg ggtattcaac
    15601 acctaataca ggagcactaa aattattaga cccagaaata actcagaatc ctgttgcata
    15661 tccatctaaa gaagtacttg ataaatgtga aacttttgaa gatattggtg aaaatataaa
    15721 attatatgac aaggcttgga tagagttaaa atcaaaatag ggtcttgttg gtttaagtaa
    15781 atttacttaa taaatactta ataaaactgt tcttttttag aaaatttatt ttctaattat
    15841 tgaacagttt tattttttgt ttaggtcatg tttttatttt gagtttattt ttatgagaat
    15901 ttaaagtaca gtaagtttca ttgattacta tttttataat aataactata aagaggttgt
    15961 ctcgaaattg agataagcct ctttttcatt gttgaaatga tgaaaacatg tttgatttca
    16021 taaaaaaaga gtgattcatg agctaaagaa tttgctttga gacagtattt tttattaagt
    16081 tatattcata tgcaatttaa atagtttatt aataaatgaa aacttatttg tataatgatt
    16141 ttaataataa ttagaaaaaa tgggcaactt aatagaataa ttggaggtgt aaatttataa
    16201 aaaatgaata ggataaataa aaaaatagat ttattaatat tggctttgct cgtttttata
    16261 tttttaattg ttggaatagt aatactatcg attaggacag aaaataaatt gaatgtgttc
    16321 ataatgttag caataatttt ttttataata atgttaactt atttatcaaa ttcagtagtg
    16381 gggttgataa caagttcaat aattatattt atgtatacat cctatatttt atataacaat
    16441 attactcaca atatggatgt tgaatttata tcatatatgt ggataatagc aacaccagta
    16501 tcttcaatta taatgggtaa tctgaacaaa agcataaatg aattgcaaaa tacaaataaa
    16561 aaactatctg aacagtataa ggaattagtg actattgata gtgaaactgg acttagaaat
    16621 ttaaagattt tttataatga tgtaaatatg gagataagta aatctattag acataatact
    16681 gatttttctc ttatgatagt aaaattacca tactacggaa acctacagac tatatttggt
    16741 gaaaacaaaa ctaataaaat agtaaaatat attggttcaa atattataga atgtacaaga
    16801 aatgaagata taatttatag tttacaaaaa gatatgatag ggatactaat gccaaataca
    16861 tctttggaag gttcgaaagt tgttaaagac agaataaaga aaaggataaa agaattaaat
    16921 ttagatttaa acaatagggg aaaatatgta aatatagatg tcaaaattgc atttttacaa
    16981 tataagagta gttttggaga tagtataaat tttaagaata tagttgaaga ggaacttcaa
    17041 tatgatgttt aaaatattta ataaaagttt attaaaatta gtaattgtat tatcaatagt
    17101 actgacaata tcaaatttta gcttattttc atatgcatca gaaattactt ccagcacaga
    17161 taaggtatta atcatatatg attctaagaa agaaacagct tataatagag atattttaaa
    17221 tattatgaga actctattgg gaagatttag ttcagatata gagctattaa aattgagtaa
    17281 ctatgatggt gagataaatg aaaattatta cagtcatata tttattttag gaatcaatga
    17341 aaactcatat aataatgata aaaacactaa aaacttgatt agttccttaa attcatataa
    17401 agtaactata tgttggttag gatatggtat agaaaatctt ttggaacata aaaaatacaa
    17461 tttagactat gtaggaaaaa caaacaatat agttagtgta aattatagag gtaaatcata
    17521 taacttggat gaacattatg tttttaacat tgtagaatca aaagatacaa gtaataaagt
    17581 aataggaagt attaatgata ccttaaataa gtatccatat ataataaatg ataaaaatct
    17641 tttttatgta tcaaaattag atttagatgg agttttattt tatatatttt gcgattcact
    17701 taatgatatt tttaatatta agacgtttga taaaggacgt atatttgtaa gaatagaaga
    17761 tgtacatgct tttagagaac caaaaaatct tgtagagata gcggattatt taagtagtaa
    17821 gaatatacca tttacgattg cattaatacc agcatatgta aatcctaaaa atcataaagt
    17881 aataacttta tcagaaagcc ctgaaattgt caaagcaata aaatatatgc aagataaagg
    17941 tggaactgtg atattgcatg gatatactca tcaatacaag aaagaagaag tttctggaga
    18001 agggtatgaa ttttgggatg gtaaaaaaga tgaaccctta aaagaaaata tgaagatatt
    18061 tgtcaaagat agagttttga atggtcttag agtatgtata gagaatggaa tttatcctct
    18121 tgcatttgaa gcgccacatt atgcaatgga atcagaggga tataaagaat taaaaaaata
    18181 tttttctact tatatggggc aacatcaaaa taatgataaa aaatttagca caaatacata
    18241 tccttatatt attagggata cagaggagtt taatatattt atacccgaaa atttaggata
    18301 tatagaccca gaagacaagt ttacttttca aaatataaaa gaaaatttag ataaattatc
    18361 tattgtaaga ggttttagtg gagggttctt ctttcattca tatttaaaca tagagtatct
    18421 aaaaaacaca atagaatatt tagaaaaaca gaatatagaa tttatgaatt taagagattt
    18481 taataattgg gtaaaagttg atgaaataca aataagaaat aatggagatg aaattatagt
    18541 caactatgat aaggacttag atgaaataac taaaagtgat actagattta aatacatatc
    18601 caatattagt aaaattttaa tattca
//
LOCUS       DAMJWP010000056        18376 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.057, whole genome shotgun sequence.
ACCESSION   DAMJWP010000056 DAMJWP010000000
VERSION     DAMJWP010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18376
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.057"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            191..2947
                     /locus_tag="PC700_001646"
     CDS             191..2947
                     /locus_tag="PC700_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2580959.1"
                     /translation="MRDEILSFVNECVLFNNFDELKKESGIITEDIVKKFNLSRTQSS
                     RLLNDLVKSDNLIKINSRPVIFLPKNKINDVVGPTKKSIYENLDELKNECKLLKQETV
                     FNSIIGYDNSLKEVIEQIKTAVLYPNGGLTIMLTGESGVGKTFLAKIIYKYSVSSNVL
                     EKNSPYNVLNCAQYYNNLELLSSLLFGHVKGAYTGANESRAGLIEQSDGGVLFLDEVH
                     RLNSEGQEKLFTFMDTGTFSRIGENGVIRKARVRLIFATTESLSKFLQTFLRRIPINI
                     YVPNIEERGTIEKRKIVEHFIYEESKVLGLPIEITQITLNTILKIKFKGNIGECKNVI
                     KYACGRAYAKNQRRNGNIFVTLQDLPRNLYIENVDIFQFQSGKSKSIIFSPKQFSIEM
                     SNGRNKRNIIYQSFIKCLDYFDRLEKNEITKEIFSKKSSNLMIRLMDNLVFSNNDENN
                     NVLMELIISIMQDIFRNLEVNNNIKYDGNNVLAFSSYLYLKDESYQLSDEYKALESKL
                     LIYLKKESYKEYSIISKISTLITNRLDMYLNNVDTIIMIIFIKSTIIRMKFNQVNATI
                     IAHGYATASSMANVCNRMLGKNIFTAIDMPIDATIADVSKQMSRYLEGDRIKNGLIVL
                     FDMGSLNLIYEDLKNKINGPMLFIDQISTISALEVGNLLMQGKNIEQIAEIMDEETKP
                     NIQLYYPQKNKEYAIISSCFTGIGTAIKIQHLLSESLIGIVDVKIIPYDYDSLVSMGD
                     KEPVFEMYDVMAIVGTANPNVNGVDFILLEDIISGKGEDDVFRIFSRVVENEKIKNIN
                     DSIVKNFSLIRVIDSLTILDSKKIIERIEEGINAIENIQKKKLPNDKKISLYVHLSCM
                     IERLVRQTEIEEYTDMDLLIKNHHSEVKLIKNAFSVLEKSYSVQIPISEIGYIYNIIY
                     GLPQ"
     gene            3079..3558
                     /locus_tag="PC700_001647"
     CDS             3079..3558
                     /locus_tag="PC700_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2580960.1"
                     /translation="MVSLMRIDDRLIHGQVAYGWATSLGINVILVANDEAKNDQMKAM
                     SLNLAKPSNVTLYIRGVEESGEIVEKFASSKKSNVLVLVKNTRDALVVAKNSGGVLKE
                     INVGGLRYEEGKKKLTDLVAVDDDDINNFKEIGDLGIDIELRMLPRDKKKQLSDLIK"
     gene            3574..3996
                     /locus_tag="PC700_001648"
     CDS             3574..3996
                     /locus_tag="PC700_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HDF2580961.1"
                     /translation="MKKFLIATHGELSRELVDTSKLIIGSLANIEYFCMTKDKSADDA
                     EKEIRSILSNNKDSEEFVVLTDVFGGSVANICTNLLLQGFKFELLTGVNLPMLLSIFL
                     LVEEDTKLIVKSGLEEAKNGIVYVNELLENRNKIKNVM"
     gene            4036..4827
                     /locus_tag="PC700_001649"
     CDS             4036..4827
                     /locus_tag="PC700_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2580962.1"
                     /translation="MLVQATMLAIIAGVGILDGRIFGQSMFDRPIVTGMLVGLVLGDI
                     KSGIMIGAQLELIWMGIAGIGAATPPDVVTGGVLGTAFAILSGNGAEVALAVAVPVAV
                     LAQSLGVLVRIINSYFSQKAVFYAKQADFKKVTIMMWLPVILFFLSTSVPTFLAIMIG
                     ADKVTTLINAIPKVILDGLGVAGTLLPAVGFALLMDMLISKKMSVFFFIGFLLASYGG
                     LDITAIALFGVCVAFVLNFYINNKENNQNPQLAMNTLTEGEIDFE"
     gene            4820..5620
                     /locus_tag="PC700_001650"
     CDS             4820..5620
                     /locus_tag="PC700_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF2580963.1"
                     /translation="MNKLTKKELRSMFWRSFALQGAFNYERMQNLGYCYSMLPAIKKL
                     YNKKENQAKAIERHLEIFNTTTVVVPAILGITAAMEEENANNPEFDESSISAVKTALM
                     GPLAGIGDSLFWGTFRIIAAGVGVSLAKEGNIFGPLLFLLLYNIPAFALRIFGLKYGY
                     KVGVNSLERIQREGLMEKIMSMATTVGLFVVGGMVATMLSITTPLKFNLNGAEVILQD
                     ILDKIIPNMLPLTFAFLIYYMLKRKVSVTKLTIGTIVTGIALHAIGLL"
     gene            5668..6723
                     /locus_tag="PC700_001651"
     CDS             5668..6723
                     /locus_tag="PC700_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF2580964.1"
                     /translation="MTIQDYMLETPVRMREIISNADSLFNEVKRANLKKIIITGSGTS
                     YHSGVQVQPYLQNLLDIDVVKMYPFMITEDTFKFDNENTLVVGVSQGGSSYSTYNAMK
                     LAEDKGCKIASMAGCKNALIDEISDYILTVNCGEEKSGAKTKGYYCTKLNLMLLGLQI
                     AREKGTISSEKYNEEINKILDAINRFEAVYKLSKQWIERNKEKLVNSKEIRIIGHSDI
                     YGDTLEAALKLLETMRIPVTGYEFEEFIHGIYNAINSDSTIFILDTGKEPRVTKMIDV
                     LSGWTENVFAIGRDVTENDKNLKIDITDNPYYQTFNFIVPIQLICGEIPTLRGVDPSV
                     PKDTRFHMKLGSKKLNK"
     gene            complement(6883..8829)
                     /gene="feoB"
                     /locus_tag="PC700_001652"
     CDS             complement(6883..8829)
                     /gene="feoB"
                     /locus_tag="PC700_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDF2580965.1"
                     /translation="MINVALLGNPNVGKTTVFNLLTGSNQYVGNWPGVTIEKKEGFLG
                     KEIKVVDLPGIYAMDTFSNEEKVSKSYLENEDVDVIVNVVDASNLSRNLYLTTQLMQF
                     NKPIVILLNMLDIAESKGVNIDAKKLSEELGVIVVPIIAKKKNGVDRIEEEIKGATQK
                     AFIYQKNFGSETETYKKLESILSRCTKTTYTQKKSISDKIDNIVLNPILAYPIFIGVL
                     FLLFKFTFDWVGGPLQEGFASLIEAYISAPVSDMLSNSSPWFKSLIVDGIIGGVGGTL
                     PFFPLIFTLFFGISLFEDSGYMSRTAFLMDKVMRKVGLSGKAFIPIVMGMGCSSPAIM
                     ATRTLESEKDRRVTALIAPLMTCGAKLPIYALFVAIFFPHNAALVTTSLYLVGIVMAI
                     VVALFLNKTAFKTEAEPFILELPEYRIPTIDALIKNTWNKSKGFLIRVVTVMFAMSVV
                     IWGLSSFNVFGYTEDINVSFLATLGHIISPIFKPLGFDDWRTSVAILSGLGAKEIVVN
                     SLNILYGNLTVVLPTIFNGVTAYTFLIFTALYTPCIAALATMKKEYGNKMMFTSFAYQ
                     FILAWIMAFIVKSIGGVLFMGNSIMEALIGGIIIVLALFILFNKFKSTKNGNVCSGCS
                     ICSGCPSANSCSSKEDSNKKSNTH"
     gene            complement(8847..9065)
                     /locus_tag="PC700_001653"
     CDS             complement(8847..9065)
                     /locus_tag="PC700_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF2580966.1"
                     /translation="MTVYDLKIGEKGFINSIDGDIKLSKRLLALGCINSTEIEVKKVA
                     PLGDPIIIRFRGFDLAIRKSDAKNISLQ"
     gene            9271..9780
                     /locus_tag="PC700_001654"
     CDS             9271..9780
                     /locus_tag="PC700_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HDF2580967.1"
                     /translation="MKTRKMTFLGLMIGYSLALYVLETYIPNPLIVIFPGAKLGLSNI
                     ITLISLMLLGVKDTVIIVTIRVILSSIFAGPLSYLLFSIGGAYLSLLFMYLASKIKDL
                     SLIGISIIGAIGHNIGQLIVASLIIENILAIGYLPFMLMASLVTGMFVGLVSRYTIPK
                     LKHFYSKFY"
     gene            9858..10019
                     /locus_tag="PC700_001655"
     CDS             9858..10019
                     /locus_tag="PC700_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HDF2580968.1"
                     /translation="MANNELEALKNEIEAVREEINTYIEYPEIFQEELVESSKKIDIL
                     INKYMYLSK"
     gene            10170..10835
                     /locus_tag="PC700_001656"
     CDS             10170..10835
                     /locus_tag="PC700_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDF2580969.1"
                     /translation="MIDIGSKEIAIRLLLALIIGGIIGLEREKIRQFAGFRTHILIAI
                     GSCIVSITSIQLFFDYNLYTNADPARMPAQVLSGIGFLGAGAILKNKGGVKGLTTAAG
                     MWATACIGIAVGYGYYELAILGGLFVMIALFVLRIINTFLFSQEKNVLILKLSNLDSI
                     PFLYDKFEKNQLIVRNMEIECRNEEYWKVNFFVSYAKRIHLHDVMKEINMIEGVINVD
                     YLD"
     gene            10901..12685
                     /gene="pepF"
                     /locus_tag="PC700_001657"
     CDS             10901..12685
                     /gene="pepF"
                     /locus_tag="PC700_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDF2580970.1"
                     /translation="MGTDRSTIEEKYKWKIDKMYSSKEEIEKDISKVKNLIQEVKEYK
                     GKLSESKENLYKVLNISENASRIVQNLYVYTHMKQHEDTRINDNQGRATKTEMLSTDL
                     GVATSYIVPEILAIEENKLSEYLKDEKLSFYTKYIKDILRDKPHTLSEREEEILAATS
                     ELSTIPENVYDMLAYADMEFPEIEDEEGNKVKLSHSNYSLFIKSKNSRVRKDAFEGEF
                     STYEKYKNTFASTLYGGIKSEIFYAKTRKYNSAIEASLFSDDVSLDVYNNLISAIDKS
                     IPNLNKYVELKKKFLGLNEIHMYDLYVPLTDKFDMDIPYDKAQDIILEALKPLGEKYL
                     KVIKSAFDEGWIDVYDNEGKKGGAYSWGSYDSHPYILMSYKNDLNSLFTLIHELGHSV
                     HSHYSRTSQPFLYSDYKIFVAEVASTLNELLLINYLLENSKSKDETIYLLNYYLEQFR
                     TTVHRQTMFAEFEKIVHQRVESGEPLTADEFTNIYYKLNEKYYGKSAIVDKQIGIEWA
                     RIPHFYSNFYVYKYATGFSAASALSQQILSEGSTAVDRYINFLKSGGSEYPLEQLKSA
                     GVDMTKKESIEEALNVFAELVNKLEKEL"
     gene            12937..13779
                     /locus_tag="PC700_001658"
     CDS             12937..13779
                     /locus_tag="PC700_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2580971.1"
                     /translation="MFKKKIGVLLASTMLVGALAVGCSSNSGGSGDSGDSSKVSVSGS
                     TSIGPLMETIGEKFQEKNQGVSVEVQQIGSSGGIKNAIEGTSEIGMSSRDLKDEEKSA
                     GLKETKIAIDGIALITNTANSVKNLTMDQVKGIYTGKITNWKEVGGNDAPIVIVSREE
                     GSGTRDGFQEIVGFDSEELTKDAQISDGSGNIKTTVEGNENAIGYISFGYVDEKVNSL
                     KINDVEATDANVMANKYPISRPFILVNKEDGLSEKGKALIDYILSDEGQALVSEKGFI
                     KVNQ"
     regulatory      14012..14095
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            14675..15352
                     /locus_tag="PC700_001659"
     CDS             14675..15352
                     /locus_tag="PC700_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2580972.1"
                     /translation="MYNLLVVDDDVDILNINQIYFSNIGFNVYTSETAKDAMDIVERI
                     VLDCIILDISLPDIDGYKMCNDIKEKVNIPIIFLSNYEHEEEKVRGFLAGGDDYITKP
                     YSLKELELRIYARMRQYKNITSVPNVLKFSALVINVNSRKVTFDGESIDLTTMEFEIL
                     LFLAENKEQVFSKIEIYNQVWKMPDIGDQHTVQVHIAQMRKKINSLSREHQYIQTVWG
                     KGYKFVP"
     gene            15424..17439
                     /locus_tag="PC700_001660"
     CDS             15424..17439
                     /locus_tag="PC700_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF2580973.1"
                     /translation="MKKYNILKGTLIVLIVSLLAIAGLNLLYKYDNKYTYKSIRASNG
                     ILTIDNDTFTKGKLVFLIDDWEFYNHKIFKPNDFTEQDIKPEYVFIGQYPDFSMRKGN
                     QSPYGNATYRMRIKNEGEDKLLSLELPEIFSASEVWINGEIVSKLGDVGTEKYRPKIK
                     NSVVSFIAKKDTEIIINVSNFSHYYSGLYYPPALGETKDISNMMFYRLLFYSIICFTT
                     FAIAIFSLSVWFLSNKSKLYLYFGCMCVFFAIHVSYPFVHLLGLPLVSFTYAVEDSSY
                     FMVVLCIICINGSISKIEEDKVYRFLILPLGITMSIISIVIPIILLPYDMYFVNIYGK
                     LIDIYKYIVFIYILMSSLVSIYKKTCSEHISSYILVSVNTVFGISILFDAVTSNRFEP
                     IFTGWQTEYCGFIIVILFSLMMINRNRIIIKENEKLTRNLEHEVEVRTNELTTLLNER
                     KKFLADVAHDLKAPVSAIQSFIDLIKVGNIHVDEETRTYLYAINQKSNEVQNRVRSLQ
                     EFTSQDKSIGISEKICLNDFIKEIYENNLPDTQACGVNFETYILEKKIYAKINKEMLT
                     RAFENLIYNALTFTPFDGKIILSMDKEDGFAIIKVSDNGKGIPPENIPKVFDRFFTER
                     DNNEPKGQGLGLYIVKSIIREHGGEIFVDSELGKGTVFTIKLSLCFL"
     gene            17489..18169
                     /locus_tag="PC700_001661"
     CDS             17489..18169
                     /locus_tag="PC700_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2580974.1"
                     /translation="MQTKILIIDGDKDNCQKLKGFLEEKEISIDLAYNCEEAIGKIFS
                     NKYDLIFLEIILTDGDGWTLCKKIRNVTTCPIVYMTYINEEQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRMNSYVNNNENNTKLEEYNRTSGVIKLEDKIIKLTPTENKLL
                     NFFIENPEKTLTTKEIYEHVWMNEYLEDNYSVVVAVNGLRKKIEKDYKNPQKIITIRG
                     AGYYFNKE"
BASE COUNT         7189 a         2029 c         3157 g         6001 t
ORIGIN      
        1 tatgtcagat atatggaaag ataagaacct tgagattatg ttaaaaaatc tagattttat
       61 acttataaaa agtttgattt tacaacctta caaaagagaa tcaagatagg aatataatta
      121 tctttttcat aaatatataa atgatatact aatattatag tattattgat atttgtattg
      181 gaggttgaac atgagggatg aaattttaag ttttgtaaat gaatgtgtat tatttaacaa
      241 ctttgatgaa cttaaaaaag aaagtggcat aataacagaa gatatagtaa aaaagtttaa
      301 tttaagtaga actcaatcaa gtagattatt aaatgattta gtaaaatctg ataatcttat
      361 aaagataaac agtagacctg ttattttttt acctaaaaat aaaataaatg atgtagtagg
      421 acctacaaaa aaaagtattt atgaaaattt agatgaactt aaaaatgagt gtaaattatt
      481 aaagcaagag actgtgttta atagtatcat aggatatgat aatagcttaa aagaagtaat
      541 cgaacaaata aaaaccgctg tactttatcc aaatggcgga cttacaataa tgctgacagg
      601 tgaaagtgga gtaggaaaaa cttttttagc caagataata tacaaatata gtgtaagcag
      661 taatgtatta gaaaaaaatt ctccatacaa tgtgttaaac tgtgcgcagt attataataa
      721 tttggagtta ttgtctagtc tattatttgg gcatgtaaaa ggagcatata ctggagcaaa
      781 tgaatctaga gcaggtctaa tagaacaatc agatggtggt gttttatttt tagatgaagt
      841 acatagatta aatagtgaag gtcaggaaaa gttatttaca tttatggata caggtacgtt
      901 ttctaggatt ggagagaatg gagtaattag aaaagctagg gtaagattaa tatttgcaac
      961 aacagaaagt ctatccaaat ttttgcaaac ttttttaaga agaataccca taaatattta
     1021 tgttcccaat atagaagaaa gagggactat tgaaaaaaga aagattgtag aacattttat
     1081 ttatgaagaa agtaaggtac ttggcttgcc tatagagata actcaaatta cattaaatac
     1141 gattttaaag ataaaattca aggggaatat aggtgaatgt aaaaatgtaa taaaatatgc
     1201 atgtggacga gcttatgcaa aaaatcaaag aagaaatgga aatatttttg ttactcttca
     1261 agacctccct agaaatcttt atatagaaaa tgtagatata tttcaattcc aaagtggtaa
     1321 aagcaaaagc attatctttt ctcctaaaca atttagtatt gaaatgtcaa atggaagaaa
     1381 taaaagaaat ataatctatc aaagtttcat aaaatgttta gactactttg atagactaga
     1441 aaaaaatgaa ataacaaaag aaatatttag taaaaagagt tctaatctaa tgattagact
     1501 aatggacaat ttagtctttt caaataatga tgaaaataat aacgtactta tggagcttat
     1561 aatatcgata atgcaggata tatttagaaa tttagaagtt aataacaata taaaatacga
     1621 tgggaataat gtattggcat tttcgtctta tctttatttg aaggacgaat cttatcaatt
     1681 atctgatgag tataaagctt tagaaagtaa attactaata tatttaaaaa aagagagtta
     1741 caaagaatac tctataataa gtaaaatatc aacattaata acgaatagat tggatatgta
     1801 tcttaataat gtagatacaa tcataatgat tatatttatt aaatcaacaa ttataagaat
     1861 gaaatttaat caagttaatg cgactattat agcacatgga tatgctactg ccagtagtat
     1921 ggcgaatgta tgtaatcgga tgttgggtaa aaatatattt acagctattg atatgcctat
     1981 agatgctacc attgctgatg tatcaaaaca aatgtctaga tacctagaag gagataggat
     2041 aaagaatggt ttaattgtgt tatttgatat gggctctctt aatcttatct atgaagattt
     2101 aaaaaataag ataaatgggc caatgttatt tatagaccaa atatccacta tttctgcact
     2161 agaagtagga aatttattaa tgcaaggtaa aaacattgaa cagattgcag aaattatgga
     2221 tgaagaaact aaacctaata tacaattgta ttatcctcaa aaaaataaag aatatgctat
     2281 aatatcatct tgttttacag ggataggtac tgctataaaa atccaacatc tactttccga
     2341 aagtttaatt gggatagttg atgtcaaaat aataccttat gattatgata gtcttgtttc
     2401 tatgggagat aaagaaccag tttttgaaat gtatgatgtg atggctatag taggaactgc
     2461 taatccaaat gtgaatgggg tggattttat attgttagag gatataatat caggaaaagg
     2521 agaagatgat gtttttagaa tatttagtag agtagtagaa aatgaaaaaa taaagaacat
     2581 aaatgattct attgtaaaga atttttcatt aattagagta atagacagtc taactatttt
     2641 agattcaaaa aagataatag agagaataga agaaggaatc aatgctatag aaaatattca
     2701 aaagaaaaaa cttccaaatg ataaaaagat ttctttatat gttcatttaa gttgtatgat
     2761 tgaaagatta gttagacaaa ctgaaattga agagtatact gatatggatt tgttaatcaa
     2821 aaaccatcat agtgaagtaa aactaataaa aaatgcattt agtgtgctag aaaagagtta
     2881 tagtgtgcaa ataccaattt cagagatagg atatatttat aatattatct atggtctacc
     2941 acagtaaaat acaagattac aaaataagta aatgaaattt aaaatagtat gccagtattt
     3001 ggcatacttt ttgcttttat agataaatga gagtataaac aataaaaatt ttaatgtatg
     3061 atggaggatt tggggaaaat ggtatctttg atgagaattg atgacagatt gatacatggt
     3121 caggttgcat atggatgggc aacatcacta ggtataaatg taatactagt tgcaaatgat
     3181 gaagcaaaaa atgaccaaat gaaagctatg tcactgaatt tagcgaaacc tagcaatgta
     3241 actttatata taaggggggt agaggaatct ggagaaatag tagaaaaatt tgcttcttca
     3301 aagaaaagca atgtattagt gttagtaaaa aataccagag atgctttagt tgtagctaaa
     3361 aattcaggag gagtattaaa agaaataaat gtaggagggt taagatatga agaaggaaaa
     3421 aagaaactta cagatttagt tgctgttgat gatgatgata taaataactt taaagaaata
     3481 ggtgatttag gaattgatat agaacttaga atgttaccta gagacaaaaa gaaacaacta
     3541 tcagatttaa taaaataatt taggagaaag agaatgaaaa aatttctaat tgcaacacat
     3601 ggagaattgt ctagagaact tgttgacaca agtaaattaa taattggttc attagctaat
     3661 attgagtatt tttgtatgac aaaagataaa tcagcagatg atgctgagaa agaaattaga
     3721 agtatattat caaataataa agatagtgaa gaatttgttg ttttaacaga tgtgtttggt
     3781 ggaagtgtgg caaatatatg tacaaactta cttttgcaag ggttcaaatt tgaattgttg
     3841 acaggcgtaa atttaccaat gcttttatct atattcttat tagttgagga ggatacaaaa
     3901 ctaatagtta aatcaggctt agaggaagca aaaaatggaa ttgtttatgt aaatgagtta
     3961 ttagaaaatc gaaataaaat caaaaatgta atgtaattta atgtaaatta aactaagtta
     4021 aaatggaggt tcaatatgtt agtacaagct actatgttag ctattatagc aggagttggt
     4081 atactagatg ggcgtatatt tggtcaatct atgtttgata ggccaatagt tacaggtatg
     4141 ttagttggat tagttttagg agacataaaa tcaggaatta tgattggtgc acagcttgaa
     4201 ttaatttgga tgggaattgc agggataggt gcagctactc ctccagacgt agtaacagga
     4261 ggtgtattgg gtacagcttt tgctatatta tcaggtaatg gtgcagaagt tgctttagca
     4321 gtggcagtgc cagtagcagt attagcacaa tctcttggag tattagttag aataataaat
     4381 tcgtactttt ctcaaaaggc agttttctat gctaaacagg ctgattttaa gaaagttacc
     4441 ataatgatgt ggttgccagt aatattattc tttttaagta catcagttcc aaccttttta
     4501 gcaattatga ttggtgctga taaagttaca acattgataa atgcaatacc taaagttata
     4561 ttagacggat tgggtgttgc aggaacattg ttgccagcag taggttttgc gttgcttatg
     4621 gatatgttga tttcaaaaaa gatgtctgta tttttcttta tagggttttt gttagcatct
     4681 tatggtggat tagatataac tgctatagct ttatttggag tatgtgtagc atttgtacta
     4741 aacttttata tcaataacaa agaaaataat caaaatcctc aattagcaat gaatacttta
     4801 acggaaggag agattgactt tgaataaatt aacaaaaaaa gaattaagaa gtatgttttg
     4861 gcgttcattt gctcttcaag gtgcatttaa ttacgaaaga atgcaaaact taggttattg
     4921 ttattcaatg ttacctgcaa taaagaagct atataataag aaagaaaatc aagcaaaagc
     4981 aatagaaagg catttagaga tttttaatac aacgacagtg gtagttcctg ctatattagg
     5041 tataacagca gctatggaag aagaaaatgc taataatcca gaatttgatg aaagttcaat
     5101 tagtgctgtt aaaacagctc ttatgggacc tcttgcagga attggtgatt cattattctg
     5161 gggaacattt aggattatag cagcaggagt aggagtatca cttgcaaaag aaggcaatat
     5221 ctttggacca ctattattct tattattata caatatacca gcatttgccc ttagaatatt
     5281 tggacttaag tatggttata aagtaggggt aaattctctt gaaagaatac aaagagaggg
     5341 acttatggag aagataatgt ctatggctac aactgtcggt ttattcgttg taggaggaat
     5401 ggttgcaact atgttgagta taactactcc tttgaaattt aacttaaatg gtgcagaagt
     5461 aattttacaa gatatactag ataaaataat acctaatatg ttacctttaa cctttgcatt
     5521 tttaatatat tacatgttga agagaaaggt aagtgtaaca aaattaacta taggcactat
     5581 agtaacagga atcgctttac atgctatagg tttgttatag aaattaatta taatttattg
     5641 aatatataga taaggaaggt gtataaaatg acaatacaag attatatgtt agaaacacca
     5701 gttagaatga gagaaattat atctaatgca gatagcttat ttaatgaagt taagagagca
     5761 aatctaaaaa agattataat aacaggttca ggtactagct atcactcagg agtgcaagtg
     5821 caaccatatt tacaaaattt attagatata gatgttgtga aaatgtatcc atttatgata
     5881 actgaagata cattcaagtt tgataatgaa aataccctag tagtaggtgt atctcaaggt
     5941 gggagtagtt attctacata caacgctatg aagttagctg aagataaggg ttgtaaaata
     6001 gcatctatgg caggttgtaa gaatgcttta atagatgaga tttctgatta tatattaaca
     6061 gtaaattgtg gtgaagaaaa atctggagct aaaacaaaag gatattattg tacaaagtta
     6121 aacttaatgt tattaggtct tcaaatagct agagaaaaag gtacaatatc tagtgaaaag
     6181 tacaatgagg aaataaataa gatattagat gcaattaata gatttgaagc agtgtataag
     6241 ctctctaaac aatggattga aagaaacaaa gaaaagttag ttaattctaa agaaataaga
     6301 ataataggtc actctgatat atatggagat acattagaag cagcattaaa attattggaa
     6361 acaatgagaa tacctgtaac tgggtatgag tttgaagagt ttatacacgg tatatataat
     6421 gctataaata gtgattctac tatatttata ctagatacag gaaaagaacc tagagtaact
     6481 aagatgattg atgtactttc tggttggacg gaaaatgtat ttgctatagg tagagatgtt
     6541 acagaaaatg ataaaaattt aaagattgat atcacagata atccttacta tcaaacattt
     6601 aattttatag tacctataca actaatatgt ggggaaatac ctacattaag aggcgtagac
     6661 ccaagtgttc caaaagatac taggttccac atgaagttag gtagcaagaa actgaataag
     6721 taacaaaaaa agaggttgtc tcaaaataga gataagcctc tttttgactg ttgaaatgat
     6781 gaaaatatat ttgattttat ataaaaaaga gtgcctcatg aactaaaaag ttaattttga
     6841 gacacccact tttatcttat tttaaactaa aatatttata tattagtgtg tattactttt
     6901 tttattgctg tcttccttac ttgagcaaga attagcactt ggacatccag agcaaataga
     6961 acatccacta cagacatttc cgttttttgt agacttgaat ttattaaaca gaataaagag
     7021 tgcaaggact ataattattc cacctatcaa agcttccatt attgaattac ccataaatag
     7081 tactccacct atgcttttaa ctataaaagc cattatccat gctagtataa attgatatgc
     7141 aaatgatgta aacatcattt tattgccata ttcttttttc atagttgcaa gtgcagctat
     7201 acatggagta tataaagcag taaatatcaa gaatgtatat gcagtaactc cattaaatat
     7261 tgttggtaag acaacagtta gattaccata tagtatattt aaagagttta ctacgatttc
     7321 ttttgctcca agaccactta aaatagcaac tgaagttctc caatcatcaa aaccaagagg
     7381 tttgaatata ggagatataa tatgacctag agtagccaag aagcttacat ttatatcttc
     7441 tgtatatcca aaaacattga atgaagaaag tccccaaatt acgactgaca ttgcaaacat
     7501 gacagtaaca actcttatca agaaaccttt tgatttattc caagtatttt taataagtgc
     7561 atctatagta gggattctat attctggtaa ttctaatata aatggttcag cctctgtttt
     7621 aaaggctgtt ttatttaaga ataatgcaac tacaattgcc ataactatac ccaccaaata
     7681 cagtgacgta gttactaaag cagcattatg tggaaagaat atagctacaa atagtgcata
     7741 tataggtagc tttgctccac aagtcataag tggtgcaata agagctgtaa ctcttctatc
     7801 tttttcgctt tccaatgttc tagttgccat tatagctgga gaagaacatc ccatacccat
     7861 aactataggt ataaaagctt taccagatag accaaccttt ctcataactt tatccattaa
     7921 aaatgctgtt cttgacatgt atccactatc ttcaaacaac gatataccaa agaataatgt
     7981 aaatattaaa gggaagaatg gtaaagtacc accaacacct ccaattattc catcaactat
     8041 aagtgattta aaccatgggc tagagttaga taacatatca ctaacaggtg cagaaatata
     8101 agcctctata agactagcaa atccctcttg tagtggtcct ccgacccagt caaaggtaaa
     8161 tttaaataag aggaacaata caccaataaa tatcggatat gctaatatag gattcaagac
     8221 tatattgtct atcttatcac tgatagattt cttttgtgta tatgtagttt ttgtacatct
     8281 cgataaaatg gattctaatt ttttatatgt ttctgtttca cttccaaagt ttttctgata
     8341 tataaaagct ttttgcgtag ctcccttaat ctcttcttct atcctatcaa caccattttt
     8401 cttttttgct ataattggaa caacaattac tccaagttct tcacttaatt ttttggcatc
     8461 tatattaact cctttagatt ctgcaatgtc taacatatta agcaatataa ctataggctt
     8521 gttaaattgc ataagttgag ttgttaagta aaggtttctt gataaattag aagcatcaac
     8581 tacatttact attacatcaa catcttcatt ttctaaatat gatttagaaa ccttttcttc
     8641 atttgagaaa gtatccattg catatatacc aggtaaatcg acaaccttta tctcttttcc
     8701 aagaaatcct tctttttttt ctatagtaac accaggccag ttacctacat attgatttga
     8761 acctgtaagt agattaaaaa cagtagtttt accgacattt gggtttccta ataatgctac
     8821 attaatcacg ggtagctcct cctctattat tgtaaagaaa tatttttagc atcagatttt
     8881 ctaatcgcaa ggtcaaagcc tctaaatcta attataattg ggtctccaag tggggcaacc
     8941 tttttaactt ctatttcagt actatttata catccaagtg caagcaatct tttagataat
     9001 ttaatatctc catctatact attaataaat cctttttctc ctatttttaa atcgtaaaca
     9061 gtcattatta acacctccta atgaaattca ttatcattaa aattgtaaca agtatgatac
     9121 aaaaagtcaa gatttataga ggtaattgca cattgaaata actaatattt attctaaata
     9181 gattaaaaat attttagtct aaattaggtg tttttatata ttttgtatta taatgtaaga
     9241 tgatagataa aatttggagg agttaactta atgaaaacta gaaaaatgac atttttaggt
     9301 cttatgatag ggtatagttt agctttatat gtactagaga catatatacc aaatcctctt
     9361 atagttatat ttccaggggc aaaattgggt ttgagcaata ttataacttt aatttcatta
     9421 atgttgttag gtgtcaaaga tactgttata attgttacta tcagggtaat tttgtcatca
     9481 atatttgcag gccctttatc atatctacta tttagtatag gaggagctta tctgagtctt
     9541 ttatttatgt atttagcgag taaaataaaa gatttatcac taatagggat aagcattata
     9601 ggtgcaatag ggcataatat aggtcaatta atagttgcaa gtttaatcat agaaaatata
     9661 cttgctatag ggtacttacc atttatgtta atggcatcat tagtaactgg tatgtttgta
     9721 ggcttagtat ccaggtatac aatacctaaa ttaaagcatt tttatagtaa gttttattga
     9781 cataatattg acaaaatata atattgtttg ataaaatgta tataaaaata tacaaatttg
     9841 taatagtggt gattattatg gcgaacaatg aattagaagc gttaaaaaat gagattgaag
     9901 cagttaggga agagataaat acatacatag aatatccaga aatatttcaa gaagagttag
     9961 tagaatcaag taagaaaata gatatattaa taaataaata catgtaccta agcaaatagc
    10021 ccacagttgt gggttttttt gtatcaaaaa ttaacagatg gctatatttt acatatatta
    10081 ataataatat taagatataa aatagtattt ctgatgtgta aattatctgt aggacataag
    10141 ttgtaattaa gtcaggaggg ttttatatta tgattgatat aggaagtaag gagatagcaa
    10201 taaggttact attagcacta ataataggag gaataatagg cttagagaga gaaaaaataa
    10261 gacagtttgc aggatttagg acacatatac taattgcaat aggttcttgt attgtttcaa
    10321 taacttcgat tcaattattc tttgattata atttgtatac aaatgctgac ccagcaagaa
    10381 tgcctgctca agtattatct ggaattggtt ttttaggtgc aggagcaatt ttaaaaaata
    10441 aaggtggagt aaaagggctt actacagctg ctgggatgtg ggcaactgca tgtattggta
    10501 tagcagttgg atatggttat tatgaattgg caattctagg tggattattt gtaatgatag
    10561 cattatttgt gcttaggata atcaatacgt ttttatttag ccaagaaaag aatgtattga
    10621 tattaaaatt aagcaatttg gactctatac cttttttata tgataaattt gaaaaaaatc
    10681 aacttatcgt aagaaatatg gaaatagaat gtcgtaatga agaatactgg aaggttaatt
    10741 tttttgtatc ttatgctaag agaatacact tacacgacgt tatgaaagaa ataaatatga
    10801 tagaaggagt aataaatgta gattatttgg actaaaatat atacaatata ttttgtaaag
    10861 tgaataaaat ataaaacaaa ttaaaataga ggtgtatgat atgggaactg atagaagtac
    10921 aatagaagaa aagtacaaat ggaagataga taagatgtac tccagtaaag aggaaattga
    10981 aaaagacata agtaaagtaa aaaatcttat ccaagaagta aaagaatata aagggaagct
    11041 ttctgagagt aaggagaact tatataaagt attaaatatc tctgaaaatg catctaggat
    11101 tgttcaaaat ttatatgtat atactcatat gaaacaacat gaagatacta gaattaatga
    11161 taatcaagga agagccacta agacagaaat gttatctact gatttaggtg tggctacttc
    11221 ttatatagtt ccagaaatac tggcgataga agagaataag ttaagtgaat acttaaaaga
    11281 tgagaagcta tccttttata caaaatatat aaaggatata ttgagagata aaccacatac
    11341 tttgagtgaa cgagaggaag aaatacttgc agctacatca gaattatcga caattccaga
    11401 aaatgtatac gatatgttag catatgcaga tatggaattt cctgagatag aagatgagga
    11461 gggtaataag gttaagcttt ctcattcaaa ctactcttta tttataaaaa gtaagaatag
    11521 tagagttaga aaagacgctt ttgaaggtga attttcaaca tatgagaaat ataaaaatac
    11581 atttgcctca acgctttatg gtggaataaa atcagagata ttttacgcaa aaactagaaa
    11641 gtataattca gcaatagaag cgtctttatt ttcagatgat gtaagtttag atgtttacaa
    11701 taatcttata tcagctatag ataaaagtat tcctaatcta aataaatatg ttgagcttaa
    11761 aaagaaattt ttaggattga atgaaataca catgtatgat ttgtatgttc ctttgacaga
    11821 taaatttgac atggatatac catatgataa agcacaggat atcatattag aggcacttaa
    11881 acctttagga gaaaaatact tgaaggtcat aaaaagtgct tttgatgaag ggtggattga
    11941 tgtatatgat aatgaaggta aaaaaggagg agcttattct tggggaagtt atgattcaca
    12001 tccttatata ttaatgagct ataaaaatga ccttaattct ctattcactt taattcatga
    12061 attagggcac tctgttcaca gtcattattc aagaacaagt caaccatttt tatattcgga
    12121 ctataagata ttcgttgcag aagttgcatc tacattaaat gaattacttc ttataaatta
    12181 tttattagaa aattctaagt ctaaggatga aactatatac ttattaaatt attatttaga
    12241 acaatttaga actacagttc atagacaaac tatgtttgct gaatttgaaa aaattgtaca
    12301 tcaaagggta gagagtggag aaccacttac tgctgatgaa tttactaata tatactacaa
    12361 attaaatgaa aagtattacg gaaaatctgc aatagtagat aaacaaatag gaatagaatg
    12421 ggcgagaata cctcattttt attcaaactt ctatgtatat aagtatgcca cagggttttc
    12481 tgctgcaagt gctttaagtc aacagatact ttcagaagga agtactgcag tagatagata
    12541 tataaacttc ttaaaaagtg gagggtcaga atacccacta gaacaattaa aatctgctgg
    12601 tgtagatatg actaaaaaag aatctataga ggaagcctta aatgtatttg ctgaattagt
    12661 taataaacta gaaaaagaat tataggatgt gataggttga tagcttcaat gaagacttct
    12721 gataatatga gttgtattgt gtttatatct gcctaaagta actttaaaaa gttaatctaa
    12781 ttaagaataa atcatataaa attaagtttt tacatgtatt taacaatatt tacatttact
    12841 taaccctgct ctaatattca tttaacaaag aatctgtatt attaaaatat agaaaaatga
    12901 caacaaaaaa ttaaaatata agcgggggta agtaaaatgt ttaaaaagaa aataggcgtt
    12961 cttttagcta gtacaatgtt agttggagct ttagcagtag gatgtagttc taactcagga
    13021 ggttcaggtg attcaggaga ttcaagcaaa gtttctgttt caggttcaac ttcaataggt
    13081 cctcttatgg aaactatcgg agaaaagttt caagaaaaaa atcaaggtgt tagtgtagaa
    13141 gtacaacaga ttggttcttc cggaggtata aaaaatgcca tagaaggaac atctgaaata
    13201 ggaatgagtt caagagattt aaaagatgaa gaaaaaagtg ctggattaaa ggaaactaaa
    13261 atagcaatag atggtatagc attgataact aatactgcaa actcagtaaa aaacctgaca
    13321 atggaccaag ttaaaggtat atatacagga aagattacta actggaaaga agtaggagga
    13381 aatgatgcac caatagtaat agtatcaaga gaagaaggtt caggaactag agatggattc
    13441 caagaaattg tagggtttga ttcagaagaa ctaactaaag atgcacaaat aagtgatggt
    13501 tcaggaaata taaaaactac agttgaagga aatgaaaatg ctataggata tatatcattt
    13561 ggatatgttg atgagaaagt aaatagttta aaaataaatg atgttgaagc aactgatgca
    13621 aatgtaatgg ctaataaata tccaatatca agaccattta tattagttaa taaagaagat
    13681 ggtttgtcag aaaaagggaa agctttaata gattatatat taagtgatga aggacaagca
    13741 cttgtttcag aaaaaggatt tattaaagtt aatcaataac atgaattata atataaagta
    13801 aacatataat accctgtgag ataagctcat aaaaatgatg ctttaacttg cagggtattg
    13861 tctattattg aatgtagaaa tataaatatc tttagataaa ataggtattt tagtatctta
    13921 tatctaaaaa tatggaaata ttgacaaaat aatattacaa tgttagaata aaaaagaaat
    13981 ttatgaaaat atataaaaga tttttaataa aaatatttta gaaactgaga agtatatctt
    14041 attattgggc atctggagat atatggagtt agtggtgcaa ccggctatga atataatttt
    14101 tatattgcat agtctttttt tgctttaaaa ttaacaaaaa tgttgcaaaa tttgaaaata
    14161 agagtattag tcgttaagat ttttattgat aggtgaaatt ttggctttta aagtagccat
    14221 ttgttggaaa agggaaattt tttaaaaagt ataatactat tataataaga aaataaagtt
    14281 tttataaaca ttgagatgca aaatataaac cttttgtaac aatatattta ccaaaataat
    14341 acaatttatt ttatattata accatataaa tcatataaat caatactcaa actagccaat
    14401 agacaagttt ctagaaaaat aataaattta taagtgaaaa tgactttaag ctccattctc
    14461 caacaatcgg agactttaat tagtatttaa taataaatct taatgttcaa tacaatattc
    14521 aagaattgtt caaaaatact taagtattat agtctagttt gtaaagatat tataaaataa
    14581 taatactagt tatatatgaa aataaaacat tataatttgt aataattata aaacttgaga
    14641 ttatgattaa aatacctttg aatggagtgg tgtaatgtat aatttactgg ttgtagatga
    14701 tgatgttgat attttaaata taaatcaaat ttatttttct aatataggtt ttaatgtata
    14761 tacatcagag actgctaaag atgctatgga tatagtagag cgtatagttt tagactgtat
    14821 aattttggat atttcattgc cagatataga tggatataaa atgtgcaatg atataaaaga
    14881 aaaggttaac atacctataa tatttttatc taattatgag catgaagaag aaaaagttcg
    14941 gggattttta gctggggggg atgattatat aacaaaacct tatagtttaa aagaacttga
    15001 acttagaata tatgctcgta tgaggcagta caaaaatatt acatcagttc ctaatgtgtt
    15061 aaagttttca gcgttagtaa taaatgtaaa ttcaagaaaa gtaacttttg atggagaaag
    15121 tatagattta actactatgg aatttgaaat attattgttt ctagctgaaa acaaagaaca
    15181 agtattttcg aagatagaga tatataacca agtttggaaa atgcctgata ttggagatca
    15241 acatacagtg caggttcata ttgcacagat gagaaaaaaa attaattctt taagcaggga
    15301 gcatcaatac attcaaaccg tatggggaaa aggatataag tttgtaccat aagtgagata
    15361 ctgtaattaa taaatagttc ttgttttatt taatactact atgaaagaga tgtggttgat
    15421 gcaatgaaaa agtataatat actaaaagga actctcattg tattgatagt ctctctgttg
    15481 gcaatagcag gattaaatct tctgtataaa tatgataata aatatacata taagtctatt
    15541 agagcaagta atggaatttt aactatagat aatgatactt tcactaaagg taaacttgtc
    15601 tttctgatag atgactggga gttttataat cataaaatat ttaaaccgaa tgattttaca
    15661 gagcaagata taaaaccaga atatgttttt ataggtcaat atccagattt ttctatgaga
    15721 aaagggaatc agtcaccata tgggaatgct acttatcgta tgagaataaa aaatgaaggt
    15781 gaggataagc tactaagttt agagcttcct gagatttttt ccgcaagtga agtatggata
    15841 aatggagaaa tagtttctaa attaggagat gtaggaacag aaaaatatag acccaaaatt
    15901 aaaaattcag tagtaagttt tattgcaaag aaagatacag aaattataat caatgtatcg
    15961 aatttttcac attattatag tggtttgtat tatccgcctg cattaggaga aacaaaggac
    16021 ataagtaata tgatgtttta tcgattactt ttttatagta taatttgctt cacaactttt
    16081 gcaatagcta tattttcgtt gtctgtatgg tttctttcaa acaaaagtaa actttactta
    16141 tattttggat gtatgtgtgt tttctttgcc atccatgtat cctatccctt tgtacatttg
    16201 ttaggactgc ctttggtaag ctttacatat gcggttgaag attcgtccta ttttatggta
    16261 gttttatgta ttatttgtat aaatggaagt atatccaaaa tagaagaaga taaagtgtat
    16321 agatttttaa ttttaccatt aggaattaca atgagtatca tatctatagt gataccaatt
    16381 attcttcttc catatgatat gtactttgtc aacatatatg ggaaattaat agacatatat
    16441 aaatatattg tatttatata tatattaatg tcttcgctgg tttcaatata taaaaagaca
    16501 tgctctgagc atataagttc ttatatttta gtttcagtaa acactgtttt tggaattagc
    16561 atattatttg atgcagttac tagtaataga tttgagccta tatttacagg gtggcaaaca
    16621 gaatattgtg gatttataat tgttatttta ttttctctta tgatgataaa caggaataga
    16681 ataataataa aagagaatga aaaacttaca aggaatcttg aacatgaagt tgaagtgaga
    16741 actaatgaac ttacaacatt gttaaatgaa cgtaaaaaat tcttggcaga tgtggcacat
    16801 gatttaaagg cacctgtttc agctatacaa tcatttatag acttgataaa ggttggtaat
    16861 attcatgtag atgaagagac aagaacctat ctttatgcta taaatcaaaa gtcaaatgaa
    16921 gtacaaaata gagtacgtag tttgcaagaa tttacatcac aagataaaag cattggtatt
    16981 agtgaaaaaa tatgtctaaa tgattttatt aaagagatat atgaaaataa cttgccagat
    17041 acacaagcat gtggtgtaaa ctttgaaact tacatattgg agaaaaaaat atatgcaaag
    17101 ataaataaag agatgcttac aagagcattt gagaatttaa tttataatgc tttgacattt
    17161 actccatttg atggaaagat aattttatca atggataaag aagatggatt tgcaattatt
    17221 aaagtttctg ataatggaaa aggtatacct ccagaaaata taccaaaggt atttgacaga
    17281 ttctttacag aaagagataa taatgagcca aaagggcaag ggctagggtt atatattgtc
    17341 aaatccatta taagagagca tggcggagaa atctttgtag attcagaact aggaaaagga
    17401 actgttttta caataaaatt aagtctatgt tttttataat aatagcttat ttaatataaa
    17461 agaataatga tatttgggag tgattataat gcaaactaag atattgataa tagatggaga
    17521 caaggataat tgccagaagc taaaaggatt tctggaggaa aaagaaataa gtatagattt
    17581 agcatataat tgtgaagaag ctattggaaa aatatttagc aataaatatg atttaatatt
    17641 tttagaaata attctaactg atggtgatgg atggactctt tgtaaaaaaa ttagaaatgt
    17701 aactacttgt ccaattgttt atatgactta tataaatgaa gaacaaagca ttttaaatgc
    17761 tttaaattct ggcggagatg attacttaat aaagcctcta aatttagaaa tattatatgc
    17821 aaaagtaaaa gctattttaa gaagaatgaa ctcatatgta aataataatg aaaataatac
    17881 aaaattagag gaatataata gaactagtgg agtaataaag ttagaagaca aaataataaa
    17941 attgactcca acagaaaata agctgttaaa cttttttata gagaatcctg aaaagactct
    18001 gactactaaa gagatatatg aacatgtatg gatgaatgag tacttagagg ataactatag
    18061 tgtggtagtt gcagtgaatg gattaagaaa gaaaatagag aaagattata aaaatcctca
    18121 gaaaattata acaataagag gagcaggata ttattttaat aaagaataat aaatatatta
    18181 atctcattaa taattgttat aaatgtgaaa tgctaacgat atctaaacta aattattata
    18241 aaatagctga ctgtaattta attgataaag cctattcttc tagactttgc cagttaaatt
    18301 acagtcagtt ttattatatg aaaaaatgcg taacagaact attattttat agattatatt
    18361 tgaaaagata gataaa
//
LOCUS       DAMJWP010000057        18257 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.058, whole genome shotgun sequence.
ACCESSION   DAMJWP010000057 DAMJWP010000000
VERSION     DAMJWP010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18257
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.058"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            129..1322
                     /locus_tag="PC700_001662"
     CDS             129..1322
                     /locus_tag="PC700_001662"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDF2580975.1"
                     /translation="MDTLKELFKIGSGPSSSHTMGPQRAAERFKNENPDAESFRAILY
                     GSLAATGKGHLTDYIIEKTIAPKKVEIVWEEDIIKDFHPNGMKFEALDKDKNVTAEWT
                     VYSVGGGTIAEEGQRNSKSNSIYHLDTMDEIVKWCKENNKTLVDFVLECEPKDIKDYI
                     KTIKDAMRKSIDDGLSTDEIIPGKLLLKRRASTFYNAYKKDKSFSTLVYAYALAASEQ
                     NASGNIIVTAPTCGSAGVIPGIFFAMQDFYNYDDEKIIEALLVAGIIGNIIKTNASIS
                     GAEVGCQGEVGAACSMAAAAVAYLKGGTIDHIEYAAEIALEHHLGMTCDPVYGYVQIP
                     CIERNAMAAQRAYDAANYALLTDGSHSVSLDQVVETMKETGIDMMDKYKETAKGGLAK
                     HFFSC"
     gene            1528..2646
                     /locus_tag="PC700_001663"
     CDS             1528..2646
                     /locus_tag="PC700_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HDF2580976.1"
                     /translation="MREASYLQEQLYKHRKSLNPIAEIGRKEYKTSKYIRDYLDKLGI
                     EYEVYLETGIVGVIEGKRPKKDIAFRADIDGLHSEEGVKHLCGHDGHATILLGLIEYL
                     NDNKEDLNDNIVFIFQPAEEGPGGAKALIDEGVLRKYNIDEIYGLHIYPELPEGYVGT
                     REGYFMAQIGDIDIEIVSKSGHGAMPQNGIDGIVIASNFVSSLQTIVSRNISPIDNAV
                     LTIGRIEGGARRNIIAENIKMEGTMRCFSPEVYEKMKLRVRELARGFELAYNCKVNIN
                     IIDDYIAVKNDKDLYQEFVEAIGSDTVVELEPLMISEDFSYYQKEVPGLFFMLGSRNE
                     EKGFVNGLHNINFNFDEKICVNALNVYSKLLKYKEAID"
     gene            2777..3022
                     /locus_tag="PC700_001664"
     CDS             2777..3022
                     /locus_tag="PC700_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HDF2580977.1"
                     /translation="MKEKVQREKKITNGKNTKILTNDDIMKYEIASELGLMDKVGERG
                     WAGLTAKEAGRIGGMLTSRKKRLKKIVEEQNKKDESI"
     gene            3009..3755
                     /locus_tag="PC700_001665"
     CDS             3009..3755
                     /locus_tag="PC700_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF2580978.1"
                     /translation="MSQYSDFAFVYDELMNEVDYNGWVKYIEDIIDSEGVKVKNILEL
                     ACGTGNLTIPLTKKNYDIAGIDISDEMLSVAREKAEKEGVELVLLQQDISELDFEISD
                     LDCVLCACDGFNYITYDDDLENVFSKTYELLKKDGIFIFDISSFYKLANILGNNMYGE
                     NREDIAYMWQNYFDEEENLIEMELTFFIKDECGKFERFEEIHQQRAYTEDEIVDMLEK
                     SGFKDIKYYGDFTFDKPSEESQRIFFVCKK"
     gene            4040..4915
                     /gene="hslO"
                     /locus_tag="PC700_001666"
     CDS             4040..4915
                     /gene="hslO"
                     /locus_tag="PC700_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDF2580979.1"
                     /translation="MRDYVLRATSGNGQVRAFVATTRNTVEEARRLHETTKVATAALG
                     RTLTATSIMGLMMKNDSDKLTVIIKGGGPIGTIIATSDSKGMVKGYVGNPQVEVEDYP
                     NGKLNVAAAVGTEGVVKVIKDLGLREPYNGTYPLVSGEIAEDFTYYFAVSEQTPSVVA
                     LGVLTKEDEVEFAGGFIVQLMPDAEEETIAKLEENVAKLPSITNMLKEGKSPDDILNI
                     VLDGLEPKILDTCEVGFMCECSKERVKTALVAIGKKSLAQIIEEDKKAEVGCQFCNKK
                     YMYSEEELLEILKEM"
     gene            5306..6106
                     /locus_tag="PC700_001667"
     CDS             5306..6106
                     /locus_tag="PC700_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDF2580980.1"
                     /translation="MESKTKYIGISILILLMIFLIAKNFNGKLEIPIPESNSRKFKSA
                     AVFYPQHQDDEVLWGGSAIVDAIKQCGVDNVYVVLVSDGSGVNVFKANTKFRNLTRKQ
                     KEELRNNEFKSALRELGVKPQNVIILADIDKKEGTHYELMEKTILDFEHKFKGNVTHI
                     AHHYKYDDHIMHRKNGEVLKKLKREGKIKDDLYFVKPKYVKYIPRNERAIYKAMDFDD
                     YNKVKSACYEYKVVNSKRKMYGIGYISAHSYFEHLLNDPELTSVLSED"
     gene            6633..8873
                     /locus_tag="PC700_001668"
     CDS             6633..8873
                     /locus_tag="PC700_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF2580981.1"
                     /translation="MSNEFIILKGCKENNLNNISLKIPKRKITIFTGVSGSGKSSIVF
                     ETIAKESQRQLNERFSTFVRSFLPKYGEVKADCIENLSTPIIIDQSRLGGNSRSTLGT
                     ITDVNSFLRALYSRFGSEYIGNANMFSFNDIDGMCPHCHGLGKKLVPNMNQILDMNKS
                     LNEGAILLSGFGVGSWHWNIFAESGFFDINKRICDYSEVELEKFLHGKAEKIKIENAG
                     QTNMTYEGLMVKFNRLYLSRDGEISEATKKKLSKLLIEDRCPICDGRRLNEGVYRSLI
                     NGYNIADLTSMQIDELAEVIKSINEPEAQPLIKGIIEKLNSIIDIGLGYLTLDRETSS
                     LSGGESQRIKMVKYLNSNLVDLMYIFDEPSVGLHPKDVYKLNNLLKKLRDKGNTIIVV
                     EHDPDVIKIADHVVDVGPKAGKYGGKVVYEGSYKNLLTSGTLTGNALSKFLPIKESVR
                     EHSGYLEIKNCNKNNLKNVSIKIPKGVLTLVTGVAGAGKSTLIKDEFLKQNPSAVLID
                     QSPVSANSRSSLATYSGIMNNIRSIFSKTNGVNASLFSSNSEGACDNCKGSGIVEMNL
                     AFMESIKSTCNVCEGKKFKKEVLEYRFQRKNIIEVLEMSVLDAIEFFNLKQIKTKLQC
                     IEKMGIGYLTLGQTLDTLSGGECQRLKLASELNKESSVYILDEPTTGLHMADIENFIS
                     IIEEIVDNGNTVIIIEHNIDVIKRADWIIELGPEGGTKGGRVIFEGIPKQLCNNKISL
                     TAKYIV"
     gene            9287..10822
                     /locus_tag="PC700_001669"
     CDS             9287..10822
                     /locus_tag="PC700_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDF2580982.1"
                     /translation="MINDILTLLSERSDFFIKLSIEHLMISIISIIIAIVLGGIIGII
                     VSEFHNSSKPILGTINFLYTIPSISMLGFLIPFSGIGNTTAVIALTIYALLPMVRNTY
                     TGITNVDKNILEAARGMGSTDFQILYKIKLPLALPVIMSGIRNMVIMTIALAGIASFI
                     GAGGLGVAIYRGITTNNTAMTISGSILIALLALIFDFILGLVEKFFQRKRKKKNRKKV
                     FAILGSLILIATIFTSYLFNPSKTQTIHIATKPMTEQYILGEMLDILIEKNTGLNVEI
                     TQGIGGGTSNIQPAMENGEFDIYPEYTGTGWNMVLKKNELYTESMFSKLQSEYKTKLK
                     MQWTGMYGFSNSYALAVRKEVADKYNLKNISDLKNVSSKLTFGAEYDFYEREDGYTPL
                     CNTYGLNFKDTVDLDIGLKYQAINEGKIDVMPIFTTDGQYSVSDVIVLKDDKNFYPSY
                     QCGNVVRLEVLEENPELSEVFKMVGDILTDKEMAKMNYDVENNNLEPREVACEFLNKK
                     GLL"
     gene            10838..11599
                     /locus_tag="PC700_001670"
     CDS             10838..11599
                     /locus_tag="PC700_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2580983.1"
                     /translation="MTPIIQFKNIKKQYNDKTIIDNLNLDIEKGEFLTVIGSSGSGKT
                     TLLKMINGLILPDGGNILINQTDIKNEDLIKLRRRIGYCVQGSVLFPHMTVEENISYV
                     PNLLSKKNKLEVKSAVNKWMEIVGLPNDMKVRYPSELSGGQQQRVGIARALASSPEIL
                     LMDEPFGAVDEITRKQLQKEIKEIHKKTGVTIIFITHDIYEALILGTKTLVLNHGVVQ
                     QYDTPENILNTPANQFVDQLLNIRKSIIDDDNLKE"
     gene            11937..12805
                     /locus_tag="PC700_001671"
                     /pseudo
     CDS             11937..12805
                     /locus_tag="PC700_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438778.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            12999..13250
                     /locus_tag="PC700_001672"
     CDS             12999..13250
                     /locus_tag="PC700_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HDF2580984.1"
                     /translation="MNVENELLKNLDRLHTTELGVVRIKKNLSLETNDVVNWCKTKIE
                     SPNAIINRKGKNWYISVYDCIITVNAHSYTIITAHKEKK"
     gene            13362..14141
                     /locus_tag="PC700_001673"
     CDS             13362..14141
                     /locus_tag="PC700_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2580985.1"
                     /translation="MNNTVKEKLAESLTSDTTDILPYLPYLLQDFWELGSSPEQMLQL
                     FRKHIPFNEDTKVLDLACGKGAVSVKLAKELNIHIKGIDLIPEFIDYARRKAIEYKVD
                     ALCDFEMNDANSSVKIEHDYDCVIFGAVGDILGNYKETISKLLPTIKSKGFILIDDAY
                     VVDKSTNEQLNFERVYPTYNEWCILFKELGLKLIECIPCLELENDVEEEMNWIITRAN
                     ELIKKYPSQKDMFEQYVISQKNEYTDLQQDLVGATWILQKE"
     gene            complement(14276..14926)
                     /locus_tag="PC700_001674"
     CDS             complement(14276..14926)
                     /locus_tag="PC700_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2580986.1"
                     /translation="MNIVMINGSPKVNKNNSQYFLSELKQLIQNDNKVFTFKVDSTSK
                     YPDALNAILNCDRLIIAFPLYVDGIPSHLLHFLEKCEKFLKTQNKKPIPVYAIVNCGF
                     NEGEHNHLAIQMIDHWCKKINFTWVQGIGIGAGEMFGSLESVPLGKGPKKDLGIALKE
                     LSKNILSDNIHIDDSNKSTIFISPNFPRFLYKLMGNHQWNTQAKSNGLTKSDILKK"
     gene            complement(14923..15426)
                     /locus_tag="PC700_001675"
     CDS             complement(14923..15426)
                     /locus_tag="PC700_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2580987.1"
                     /translation="MKLLIHDLTDKELEKLLPKLDDNIKILPNTKEIHKCIGRFGCWI
                     KTPSECVVKDYYSNMGKLINESDGIIIISRCCYGGFSPFVKNVLDKSINYILPYFVIK
                     DNMMRHKSRYDKQINLKAIFYGDCITEAEKETATKLVNGNAINFNIKEHSVCFYENIQ
                     DIRGESI"
     gene            complement(15475..15990)
                     /locus_tag="PC700_001676"
     CDS             complement(15475..15990)
                     /locus_tag="PC700_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDF2580988.1"
                     /translation="MLEYIILGFLMEKELSGYDLKQIMSESTSYFFDASFGSIYPALK
                     RLETKGYIHYHEVIDGSKLKKLYSITNTGKEVFLEWLKKPINFSKTKQDHLVNIFFYK
                     YLPKEIIETNLKMLITEVELLLNKLSKQKIDVEENHCIDQYTYMYATMIYGVDYYNFV
                     INWCKDLLKNL"
     gene            16306..16797
                     /locus_tag="PC700_001677"
     CDS             16306..16797
                     /locus_tag="PC700_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2580989.1"
                     /translation="MRTIDIKGENMRELLKWVSIINRYNTNYIDRKLADIGINNSQYF
                     YVLHICNNPGITQDTIFEKILVNPSNITRALANLEKEGFITREPGVKDKRTWHLYPTE
                     KSLECYDEILKITNGYVEELLSSFEKEEQELFMSMLKKVAFLAIDMNEEAKLEAKENG
                     RKI"
     gene            16781..18184
                     /locus_tag="PC700_001678"
     CDS             16781..18184
                     /locus_tag="PC700_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2580990.1"
                     /translation="MEEKYKEPLEYEKVSKLLTKYAIPSIIAMLVSALYNIVDQIFIG
                     QGVGVLGNAATNVTFPLTTICTAIALLLGIGGASKCSLELGAKNSEKATKAAGNSICL
                     MAVFGISLFIVVSIFLTPLLKFFGSTSEILPYAHTYTKITSIGLPFLIMSTGMSKLIL
                     ADGRPKASMFCMLIGAVINTILNPLFIFVFNMGIAGSALATVIGQLISFGISVHYIKN
                     FQHIKLHKKSFKLDSIIYKHIFALGSSSCFNQLAMTVTQIVMNNTLAYYGARSVYGSE
                     IPLACVGIIIKVNMIFISIIIGISQGMQPIIGYNYGAEKYNRVKEAYKFAVGSATIIS
                     VIAFLCFQFFPRQITSIFGNGNETYFIFAEHYFRTFLFLTFINGVQIVSSTFFTSIGK
                     SVLGLFTSLTRQILFLIPLIVILPMFMGIDGVMFAGPIADAAAAMVCIYFAVHEMKSL
                     TIKQNNLMSNHNKIAEV"
BASE COUNT         6984 a         1947 c         3306 g         6020 t
ORIGIN      
        1 ccaaaatgga aataaaaaat aaattttatg agaatttaat aactaataat taatatattt
       61 aggttataat aaatatgtgc gtaaaatcta acaataagca ataataccgt aaatttagga
      121 ggaataatat ggatacttta aaggaacttt ttaaaatagg aagtggacct tcaagttctc
      181 atactatggg accacaaaga gcagcagaga gatttaagaa tgaaaaccca gatgcagaaa
      241 gttttagagc aatattatat ggtagtttgg cagctacagg gaaaggtcac ttaactgact
      301 atataattga aaaaactata gcacctaaaa aagtcgaaat tgtttgggaa gaagatatta
      361 taaaagattt tcacccaaat gggatgaagt ttgaagcttt agataaagat aaaaatgtaa
      421 cagctgaatg gactgtatat tctgtaggtg gagggactat agcagaagaa ggtcaaagaa
      481 acagtaaaag caatagtata tatcacttag atactatgga tgagatagta aaatggtgca
      541 aagaaaataa taagactttg gtagattttg tgttggaatg tgaaccaaaa gacataaaag
      601 attatataaa aactataaaa gatgctatga gaaaatctat tgatgatgga ctaagtacag
      661 atgaaataat accaggcaag ttattattaa aaagaagagc aagtacattt tataatgcat
      721 ataaaaaaga taagagcttc tcaacacttg tatatgcata tgcactagca gcttcagagc
      781 aaaacgcttc tggtaatata atagttacag caccaacttg tggttctgct ggagtaatac
      841 caggaatatt ctttgcaatg caggatttct ataattatga tgacgaaaaa ataattgaag
      901 cactacttgt agctggaata ataggtaata taataaaaac taatgcttca atatctggag
      961 cagaggtagg gtgtcaaggt gaagttggag cagcttgttc tatggcagca gcagcagtag
     1021 catacttaaa aggtggaact atagaccata ttgaatatgc ggcagaaata gcacttgaac
     1081 atcatttagg aatgacttgt gaccctgtat atggatatgt tcaaattcca tgtatagaaa
     1141 gaaatgcaat ggcagctcaa agagcttatg atgcagctaa ttatgcatta ttaactgatg
     1201 gctcacattc tgtctcctta gaccaagttg ttgaaacaat gaaagaaact ggtatagata
     1261 tgatggataa gtacaaagaa acagctaagg gtggacttgc taaacatttc ttctcttgct
     1321 aaaaataaaa ttttccaata ataattcctc tgttttgatt tatgaaacag gggaattatt
     1381 attaatggtg aattatttaa taatgcaaaa tatttttata ttcaatttag ctttttattt
     1441 ttaattagta taacatgcta tataatattt taattataaa gaaatataga aatatttttt
     1501 aataaattag aaattggagg agattacatg agagaggcat catatttaca agaacaactt
     1561 tataaacata ggaagtcatt aaatcctatt gcagagattg ggagaaaaga gtataagact
     1621 tcaaaatata taagagatta tcttgataaa ttaggaattg agtatgaagt ttatttagaa
     1681 acaggtatag taggtgtaat agaaggtaaa agacctaaaa aagatattgc ttttagagct
     1741 gacatagatg gtcttcatag tgaagaaggg gtaaaacatt tatgtggtca tgatggacat
     1801 gctactatat tacttggatt aattgagtat ttaaatgata ataaagaaga cttaaatgat
     1861 aatatagtat ttatattcca accagcagaa gaaggtcctg ggggagcaaa agctttaata
     1921 gatgaaggag tgcttagaaa atacaatatt gatgaaatat atgggcttca tatatatcca
     1981 gagttaccag aaggttatgt aggtacaaga gaaggctatt ttatggctca aataggagat
     2041 atcgacatag aaatagtgtc taaaagtgga catggggcta tgccacaaaa tggtattgat
     2101 ggtatagtga tagcttcaaa ctttgtaagc tcacttcaaa ctatagtgtc tagaaatata
     2161 agcccaatag acaatgcagt gttgactatt ggtagaattg aagggggagc tagaagaaat
     2221 ataatagctg aaaatataaa gatggaaggt actatgagat gtttcagccc agaagtgtat
     2281 gagaaaatga agcttagagt tagagaactt gctagagggt ttgaattggc ttataactgt
     2341 aaggttaata taaatattat agatgattat attgctgtta aaaatgacaa ggatttgtat
     2401 caagagtttg tagaggctat tggaagtgat actgttgtag agttagagcc acttatgata
     2461 tcagaagatt tttcttatta tcaaaaagaa gttccaggat tattttttat gttgggttca
     2521 agaaacgaag aaaaaggatt tgtaaatggt cttcataata taaactttaa ttttgatgaa
     2581 aagatatgtg taaatgcatt gaatgtttat tcaaaattat taaaatacaa agaagcaata
     2641 gattaggaag ataaattata aagattaaaa agcataaata cctaaattta aaaaaaatgt
     2701 attaataaat accccttata tatttgtata aatttatttt aattgacaaa aatacaaata
     2761 agaaggggtg atttttttga aagaaaaagt acaaagagaa aagaagataa ctaatggcaa
     2821 gaatactaaa atattgacaa atgatgatat aatgaaatat gagatagcct cagaactagg
     2881 tcttatggat aaagttggtg aacgtggatg ggcaggactt acagctaagg aagcaggaag
     2941 aattggaggt atgcttacat cacgcaaaaa gaggcttaaa aagattgtgg aggagcagaa
     3001 taaaaaggat gagtcaatat agtgattttg cgtttgtata tgatgaactt atgaatgaag
     3061 ttgattataa tggttgggtt aaatatatag aagatataat agatagtgaa ggtgtaaaag
     3121 taaaaaatat attggaattg gcatgtggca cgggtaatct tacaattcca ttgactaaaa
     3181 aaaattacga tatagctggt atagatatat ctgatgagat gcttagtgta gcaagagaaa
     3241 aggcagaaaa agaaggagta gaattagttt tacttcaaca agatatatca gagcttgatt
     3301 ttgaaatatc tgatttggac tgtgtgcttt gtgcttgtga tggatttaat tatataactt
     3361 atgatgatga tttggaaaat gtattttcaa agacatatga attattaaag aaagatggaa
     3421 tattcatatt tgatataagt tctttttata aattagccaa catattggga aataacatgt
     3481 atggagaaaa tagagaagat atagcgtata tgtggcaaaa ttactttgat gaagaagaga
     3541 atctaattga aatggaactt acattcttta ttaaagatga atgtggaaag tttgaaagat
     3601 ttgaagagat acaccaacaa agagcatata ctgaagatga gatagttgat atgcttgaaa
     3661 aatctggatt taaggatata aaatattatg gggattttac atttgacaaa cccagtgaag
     3721 aaagtcaaag aatcttcttt gtatgtaaaa aataagatat ttttgactat ataagataat
     3781 tattaaaaat aaccatgata taaaaatata gaaaatgcag ttttatagat taaaagtaaa
     3841 gtgatgatac tttatgactt ttattttcta ataattataa agttctatag ataaaaatta
     3901 aaatataata actattgctt agtaaaatta aaataaaatg ggttttgccc aaggttaata
     3961 tagataagta cttatagatt aaaattaagg tagtaatata ataaaaataa gacaattaac
     4021 gggacatgga ggaatattaa tgagagatta tgtactaaga gcgacatcag gaaatggtca
     4081 agttagagca tttgtcgcaa ctactagaaa tacagtagaa gaagctagaa gattacatga
     4141 aactacaaaa gtagcaacag cagcacttgg aagaacatta acagccacat ctataatggg
     4201 attaatgatg aaaaatgata gtgataaatt gactgtaata ataaaaggtg gaggtccaat
     4261 tggaactatt atcgccacat cagattctaa gggaatggta aaaggatatg taggaaatcc
     4321 acaagtagag gttgaagact atccaaatgg aaagttaaat gtagcagcag cagtaggtac
     4381 agaaggtgtt gtaaaagtta taaaagactt aggtcttaga gaaccatata atgggactta
     4441 tccattggta agtggagaaa ttgcagaaga ctttacttat tattttgctg tatcagagca
     4501 gactccatca gtagtagctt taggtgttct tacaaaagaa gatgaagtag agtttgctgg
     4561 aggatttata gttcaattaa tgccagatgc agaagaagag actattgcta agttagagga
     4621 aaatgtagct aaattacctt ctataactaa catgttaaaa gaaggaaaat caccagatga
     4681 tatattaaat attgttttgg atggtttgga acctaaaata cttgatacct gtgaagtagg
     4741 gtttatgtgt gaatgttcaa aagaaagagt aaaaactgct ttggttgcaa ttggtaaaaa
     4801 atcattggct cagataatag aagaagataa aaaagcagaa gttggttgcc agttctgtaa
     4861 taagaagtat atgtatagtg aagaagagtt gcttgaaatt ttaaaggaaa tgtaggctat
     4921 aaaaatagtt gtattgatat aagtaatgtt atagtgatat agtaaagtcg tattgatata
     4981 gtaattaaag tataatttat taataataga catatttata ttaaaaaata aatttaataa
     5041 atttttaagc ttaataaatt ttaagaatta agaagctttc tggctgtaat atttgcaatg
     5101 ttgcagttag aaggcttttt tattttatta tagttcgtgt caaagattgt atgaaacaat
     5161 aaaatattgt aaaataattt gtatatttag acctgattga cacaaaggtt tataagatat
     5221 ttgagttgat tatacgaaat tctatgaagt agtaaccctg tgactttaat catgggaggt
     5281 tcagaagaag gagaaaattt taaatatgga aagtaaaacg aagtacatag gaataagtat
     5341 attaatatta ttgatgatat ttttaattgc aaagaatttt aacggaaaac ttgaaatacc
     5401 tattcctgag agtaatagta gaaaatttaa atcagcagca gtgttttatc ctcaacatca
     5461 agatgatgag gttttatggg gaggaagtgc cattgtagat gctataaaac agtgtggtgt
     5521 tgataatgta tatgtggtat tagtatcaga tggttctgga gttaacgttt tcaaagctaa
     5581 cacaaaattt agaaacttaa ctaggaagca aaaggaagag cttagaaata atgagtttaa
     5641 atcagctttg agagagcttg gagtgaaacc tcaaaatgtg attatacttg cagatataga
     5701 taaaaaggaa ggcacacatt atgaattaat ggaaaagact atattggatt ttgaacataa
     5761 gtttaaaggc aatgtgaccc atatagccca tcactataag tatgatgacc atataatgca
     5821 tagaaagaat ggggaagttt taaagaaact taaacgtgaa ggtaagataa aagatgactt
     5881 gtattttgtt aagccaaaat atgtaaagta tataccaaga aatgaaagag ctatctataa
     5941 agcaatggat tttgatgact acaataaggt taagagtgct tgttatgaat ataaggttgt
     6001 aaattcaaag cgtaaaatgt atggtatagg atatatttcg gcacatagtt attttgaaca
     6061 tcttctaaat gaccctgaac ttacgtctgt attaagtgaa gattaatttc ttataagtag
     6121 taagttatgt ttgataagtt gattggaatt ttctatagtt ttgaaggtat tggaaattta
     6181 gttttattta aagatttaaa gatagaggtt tttaatgttt aaaattttaa agttaagttg
     6241 aataattgta tagaattatt tgtgaaagaa gaaacagtgt aaaacatagt atcttattta
     6301 tggttttaaa atcataggca agatactttt tttatgtaac taataccatt ttgaggttat
     6361 taacttaaga attttatata atttaacact catatataat gatgatattt aggtagaata
     6421 gatgcaatta cgcttaaata aaataactgt attttttata aaaatcaaac cctacactat
     6481 actaccagat aaaatctaca tgctataatt tgacctgtgt ttataaaaaa aggagtaatc
     6541 taataaaata gtatttcact tatgtataac taacaactat tattacgatt ttctctaaat
     6601 aatttaaaga aaaataatgc aggaggtaaa atatgagcaa tgaatttata attttaaaag
     6661 gttgcaaaga gaataatttg aacaatattt ccttaaaaat accaaaaaga aaaataacca
     6721 tattcacagg ggtgtcgggt tctggaaagt catctattgt atttgagaca attgcaaaag
     6781 aatcacaacg ccaactaaat gaaaggttta gtacatttgt aagaagtttt ttacctaagt
     6841 atggagaggt taaagctgat tgtatagaaa atctgtcaac accaattatc atagaccaaa
     6901 gtagacttgg tggaaattca aggtcaaccc taggtactat tacagatgta aattcctttt
     6961 taagagcttt atattcaagg tttggtagtg agtatatagg taatgctaat atgttttctt
     7021 ttaatgatat tgatggtatg tgcccacact gtcatgggct tggaaaaaag ctagttccta
     7081 atatgaacca aattcttgat atgaataaat cattaaatga aggtgctata ttgctttcag
     7141 ggtttggagt gggttcttgg cattggaata tattcgctga atcaggattt tttgatatta
     7201 ataagaggat atgtgattat tcagaagtag aattggaaaa gtttttgcat ggtaaagcag
     7261 aaaagattaa gatagaaaat gctggtcaaa ctaacatgac atatgaggga ttaatggtta
     7321 aatttaatcg tctatactta agtagagatg gagaaatatc tgaggctact aagaaaaaat
     7381 tgagtaaact tcttatagaa gatagatgtc ctatatgtga tggaagaaga cttaatgaag
     7441 gagtatatag gtctttaatt aatggttata atatagcaga tttaactagt atgcaaattg
     7501 atgaattggc tgaagttatt aaaagtataa atgagccaga agcacaacct ttaattaaag
     7561 gaataataga aaagttaaac agtatcatag atataggtct tggatattta acactggata
     7621 gagagacatc ttctctatca ggaggggaat ctcagcgtat aaagatggtt aagtatttaa
     7681 atagtaatct tgttgattta atgtatattt ttgatgaacc aagtgtagga cttcatccaa
     7741 aagatgtgta taaattaaat aatcttttaa aaaaattgcg tgataaaggg aatacaatta
     7801 tagttgttga acatgaccca gatgttatta agattgcaga ccatgttgta gatgtaggac
     7861 caaaggcagg taagtatggt ggaaaagttg tgtatgaagg cagttataaa aatctactta
     7921 caagtggtac tttgacaggg aatgctttaa gtaaatttct tcctattaaa gaaagtgtaa
     7981 gagaacatag tggatattta gaaattaaaa attgtaataa aaacaattta aaaaatgtat
     8041 ctattaagat acctaaaggt gtattgactc tagttactgg agtagcagga gcaggtaaaa
     8101 gtacgttgat taaggatgaa tttttaaagc aaaatcctag tgctgtactt atagaccaaa
     8161 gccctgtttc agcaaattca aggtcgagtt tggcaacata tagtggaatt atgaacaaca
     8221 taagaagcat attttcaaaa acaaatggcg taaatgcttc tttattcagt tcaaattcag
     8281 aaggagcttg tgataactgt aagggttctg gaatagtaga aatgaatctt gcttttatgg
     8341 agagtataaa gagtacctgc aatgtttgtg aaggtaaaaa atttaaaaaa gaagttttag
     8401 aatataggtt tcaaaggaag aatataattg aagttcttga aatgtctgtt ttagacgcaa
     8461 ttgagttttt taatttaaaa cagattaaaa cgaaattaca atgtattgaa aaaatgggta
     8521 ttggatacct tactttagga caaactcttg atactttgtc tggaggagag tgtcaaagat
     8581 taaagcttgc tagtgaatta aataaggaaa gttcagtgta tatattagat gaaccaacaa
     8641 caggtcttca tatggctgat attgagaatt ttataagtat aattgaagag attgtagata
     8701 atggaaatac ggtcataata attgaacaca atatagatgt aattaaaaga gctgattgga
     8761 ttattgagct gggacctgaa ggagggacaa aaggcgggag agtaatattt gaaggtatcc
     8821 caaaacaatt atgtaacaat aaaatatcat taacagcaaa atatatagta taataaataa
     8881 aatataataa aattataatg atattttaat aaatataatg ggtatataaa atgataatta
     8941 aacttaacag taataaataa tgtgagaaat attcaaattt aattaaataa agtgtcagaa
     9001 atttgtttaa attttagtat tttaacgatt ctgacctttt ttcttatttt tatggagagt
     9061 atcgaagatg gagtctctaa tagttacttt tttgtgtcta cttgcaagtt tgattaaaaa
     9121 atggctatac acacttgtga cttcagtcat gggaggttca gattataatc ttaatatata
     9181 atttgtgaaa taatttgatg acgatttgct gatgtataaa caataaagat taactacatt
     9241 aaaatttaaa tcttgttaag taaaacttat aaaaggagag agatggatga taaatgatat
     9301 tctaacctta ttatctgaaa gaagtgattt ttttataaag ctatccatag aacatttgat
     9361 gatttctatt atttctataa taattgcgat tgttttgggt ggtataattg gtattattgt
     9421 aagtgaattt cataattctt ctaaacctat attgggaact atcaattttc tatacaccat
     9481 tccatctatt tctatgctag gatttctgat accattttcc ggaattggaa atactactgc
     9541 tgttattgca cttaccattt atgcattatt gccaatggtt agaaatacat atactggaat
     9601 tactaatgtg gataaaaata ttcttgaagc agctagggga atgggcagta cagattttca
     9661 gatattgtat aaaataaaac ttccattagc cctacctgtt attatgtctg gtattagaaa
     9721 tatggtgatt atgacaattg cattagctgg tatcgcttca tttataggtg ctggaggcct
     9781 aggtgtagcc atatatagag gaatcacgac aaataacaca gcaatgacaa ttagtggaag
     9841 tatacttatt gctctattag ctttaatatt tgattttatt ttagggctgg tagaaaaatt
     9901 ttttcaacga aaaagaaaaa agaaaaatag gaaaaaagtt tttgccattc taggaagcct
     9961 tattcttata gcaactatct ttacgtctta tttatttaat ccttcaaaga ctcaaactat
    10021 tcatattgct actaaaccta tgactgaaca atatattctt ggtgagatgt tggatatttt
    10081 aattgaaaaa aacacaggtc taaacgtgga aattacacaa ggcataggag ggggaacatc
    10141 taatatccag ccagctatgg agaatggaga atttgacatt tatccagagt atacaggtac
    10201 aggctggaat atggtactta agaaaaatga attatatact gaaagtatgt ttagtaaact
    10261 acagtcggaa tacaaaacta aacttaaaat gcaatggact ggtatgtatg gtttttctaa
    10321 tagctatgca ttagcagtta gaaaagaagt tgcagataaa tataatttaa aaaatatatc
    10381 agacttaaaa aatgtatctt ctaaattgac atttggagct gaatatgact tttatgagag
    10441 agaggatgga tatactccat tgtgtaatac atatggactg aactttaaag atacagttga
    10501 tttagatatt ggattaaaat accaagctat caatgaaggt aagattgacg taatgcctat
    10561 atttacaact gatggtcagt atagtgtttc agatgttata gttctaaaag atgataaaaa
    10621 tttctaccca tcttatcaat gtggaaatgt tgttcgtttg gaagtattag aggaaaatcc
    10681 agaactatca gaggtattta agatggttgg agatattttg actgataaag aaatggctaa
    10741 aatgaattat gacgtagaaa acaacaattt agaacctaga gaagttgctt gtgagtttct
    10801 gaataagaaa ggtcttttat aaattggagg aaatataatg acacctatta tacaatttaa
    10861 aaatattaaa aaacaatata atgataaaac tataatagat aatcttaatt tagatataga
    10921 aaaaggagag tttttgactg taattggttc gtctggttct ggtaagacaa cactcttaaa
    10981 aatgattaat ggtctgattt tgccagatgg aggaaatatt ttgataaacc aaacagatat
    11041 caaaaacgag gacttaatta aattaagaag aagaatagga tattgtgtac aaggaagtgt
    11101 tctttttcct catatgactg ttgaagaaaa tatttcatat gtaccaaatc tcctaagtaa
    11161 aaagaataaa ttagaagtaa aaagtgctgt aaacaagtgg atggaaatag tgggattgcc
    11221 aaatgatatg aaagtgcgtt acccatcaga attgtcaggt ggtcagcaac aacgtgtagg
    11281 gattgcaaga gctttagctt cttctccaga aattttatta atggatgaac cttttggtgc
    11341 agttgatgag attacacgaa agcaattaca aaaagagata aaagaaattc ataaaaaaac
    11401 aggagttacg attatcttta ttacgcatga tatttatgaa gctttgattt tgggaacaaa
    11461 gactttggta ttaaatcatg gagttgttca acagtatgat actcctgaga atattttaaa
    11521 tactcctgct aaccaatttg tagaccagtt attgaatata agaaaatcta taatagatga
    11581 tgataattta aaagaataaa tttaaattta aaatcaaatt gttgtgataa aaactattga
    11641 tttagagcta acttcaagtt gtatacttga agtggaaaag ttagattaag gtatagaaga
    11701 gaatttagca gttggctgta gacgaggtaa ctgctgaagg tgctttcaat aaatggctag
    11761 aacctgtatc tattgaagat tataataaat tgaaataaga agctcaatat acagagtaga
    11821 taggcacttc aaatagaagt gtcttttgtg tgccataaaa taaatcaaaa tattagcaaa
    11881 gaatgtgaaa aacaaaataa aataatatgc tatctttaaa ataggagggg tgctatatgg
    11941 ataacaattt gattaccatt gaaaaaactt tagattatat agaaaaacat cttttagatg
    12001 agatgttaga tttaaatgta atttctaaag agataggcta ttcaaagtac catttacata
    12061 gaatgtttac atcagttgtc ggatttagta tacataatta tatacaaaga cgtagactaa
    12121 ctgaagcagc aagaatgctc atttttacag aaaaaccaat tattgaaatt gctttaatct
    12181 cggggtatga cacacaacgt tctttttcaa cagggtttaa aaaaatgttt aagcatagcc
    12241 ctaagttttt tagaaaacat caagattttt taccactaca attaaaattt gatattaata
    12301 atagaaaaag gttacatgga ggtatggttt tagatgttaa aaaagttaat tgtacagata
    12361 taaatattgt tggctattct ggtaaagtaa aaaaaggatt tcatgttatt ggaaaatgtt
    12421 ggagggattt gcataaaaat aaagagttga tagaaaacaa ggtagacttg gattttttaa
    12481 ttggagttaa tgattatagt aattttaaag aaattgaaaa taatcctaca tttacatata
    12541 ttgcaggtgt tcaagtagct acttttggta aattcctaat ggaatggaaa aattcgtatt
    12601 accgaaaagt aattatattg tttttacttt tattgggaaa aatgaggata gtatgcaaaa
    12661 tgttgtagaa tatatctatc aagaatggtt ccttaattca agttgccagt ttaatgaaaa
    12721 taatttatat gattttgtaa aatacggaga aaaaactgat aagaatggtt tgagtgaaat
    12781 tcagttttgg gttccaatca tttaaaagaa gggattatgt ataaagtagg tggatttata
    12841 taggcaaagt tttataaaag tatgtattta taaataattt atagtctgta ggaaagaaat
    12901 agttttacta taagattatg ataaaataca caatactatg ataaaatagt actaaataat
    12961 agatttaatt ttgcaaagga taaagtgttt taataaaaat gaatgtagaa aacgaattac
    13021 ttaaaaacct agatagattg catacaacag agttaggcgt tgtaagaatt aagaaaaatc
    13081 tttctttgga gacaaatgat gtagtgaact ggtgtaaaac taaaatagaa tctccaaatg
    13141 ccattattaa tagaaaggga aaaaactggt atataagtgt atatgattgt attataacag
    13201 taaacgctca cagttataca attattacag ctcataaaga aaagaaatga attgaaaaat
    13261 acaaaggtca aaagcatttt ccactgtttg tagtacctat gaagaagtat ataaaataat
    13321 attacttttg aataataatt taaattttgg aggttgaaaa aatgaataat actgtaaaag
    13381 aaaaattggc agagtcacta acttctgata ctactgatat tttaccttat ttgccttatt
    13441 tattacaaga tttttgggaa ttaggaagtt caccagaaca aatgttacaa ttatttagaa
    13501 agcatatacc ttttaatgag gatacaaaag ttttggattt agcttgtgga aaaggtgctg
    13561 tatctgtgaa gcttgcaaaa gagttgaata tacatatcaa aggtattgat ttgatacctg
    13621 aatttattga ttatgcgaga agaaaggcta ttgaatataa ggttgatgct ttgtgtgatt
    13681 ttgaaatgaa tgatgctaat agttctgtaa aaattgagca cgattatgat tgtgtgattt
    13741 ttggagctgt aggggatatt ttaggtaatt ataaagaaac tataagtaag ctattaccta
    13801 caataaaaag taaggggttc attttaattg atgatgcata tgtagtagat aaatctacaa
    13861 atgaacagct taattttgag agggtgtatc ccacttacaa tgagtggtgt atattgttca
    13921 aagaattagg tctgaaacta attgaatgta taccatgttt agaacttgaa aatgatgttg
    13981 aagaggaaat gaactggatt ataactcgtg caaatgagct aattaaaaaa tatccttctc
    14041 aaaaagatat gtttgaacaa tatgttatta gccaaaaaaa tgaatatact gatttacagc
    14101 aggaccttgt tggagcaact tggattttac aaaaagaata agataaatat tagactagat
    14161 aatcaatgtt gtagtgaatt atcccatgtc tagtgaatat aaataacata aaaaaccgtc
    14221 aagatgaata gtttattcgt gcttgacggt ttttagtatt tgttgaagta aaattttatt
    14281 tctttagtat atcactttta gtcaatccat ttgatttagc ttgagtattc cattgatgat
    14341 tccccataag tttatatagg aatctaggga agttaggtga aataaaaata gtacttttgt
    14401 tggaatcatc tatatgtata ttatcactaa gtatattttt tgaaagttct tttaatgcaa
    14461 ttcctaaatc ttttttaggt cctttaccca aaggtacgga ttctagtgaa ccaaacatct
    14521 ccccagctcc aattccaata ccttgaaccc atgtaaagtt gatttttttg caccaatgat
    14581 ctatcatttg aattgccaaa tgattatgtt caccttcatt gaatccacag ttaactattg
    14641 catacactgg aataggtttt ttattttgtg tttttaaaaa tttttcacat ttctctaaaa
    14701 aatgaagtaa atgagatgga ataccatcta cataaagagg aaatgctatt attaatctat
    14761 cacaattaag tatggcatta agagcgtcag gatacttaga tgtgctatct actttaaatg
    14821 taaaaacttt attatcattt tgaataagtt gttttaattc agataaaaaa tactgtgaat
    14881 tatttttatt tacttttgga ctaccattta tcattactat attcatatgg attcacctct
    14941 tatatcctgt atattttcat agaaacaaac agagtgttct tttatattga aatttatagc
    15001 atttccattt actaatttcg tagcagtttc tttttcagct tctgtaatac aatctccata
    15061 gaatatagct tttaaattta tctgtttatc atatctgctt ttatgacgca tcatattatc
    15121 ttttattaca aagtatggaa gaatataatt aatgctttta tcaagtacgt ttttgacaaa
    15181 agggctaaac cctccgtaac agcatctgct tattataata attccatcgg attcatttat
    15241 aagtttaccc atgttgctgt aataatcttt tacaacacat tcagaaggag ttttaatcca
    15301 gcaaccaaaa cgaccaatac atttgtggat ttcttttgtg tttggaagta tttttatatt
    15361 gtcatcaagc ttgggtaata acttttctaa ttctttgtca gttaaatcat gtattaaaag
    15421 cttcataaag tccatctcct tttattatgt atgtttattt ctaaaaatta tatcttatag
    15481 atttttcagt aaatctttac accaattaat aacaaaatta taatagtcaa ctccataaat
    15541 cattgtggca tacatatatg tatattggtc aatacaatga ttttcttcta catcaatttt
    15601 ttgtttactt aatttattta gaagtaattc tacttcagtt attaacattt ttaagtttgt
    15661 ttcaattatt tctttgggta gatacttgta aaagaatata ttaacaaggt ggtcttgctt
    15721 tgttttagaa aaatttatag gtttctttaa ccattcgaga aatacttctt taccagtatt
    15781 agttatggaa tatagttttt ttaatttgct accatctata acttcgtgat agtgtatata
    15841 tccttttgtt tctaatcttt taagtgcagg atagatactt ccaaagctgg cgtcaaaaaa
    15901 gtacgaggta ctttctgaca ttatttgttt taagtcgtat ccacttaatt ctttttccat
    15961 taaaaatcct aaaataatgt attctaacat attacctcct ggtatcaatc agatatatat
    16021 cgattcgata tatttattat tacagataat aaatattacg tcaagatgct ttttaaaata
    16081 agctaaaaag aattaggaat tattgataaa tatgcaaaaa aattaatata aaaggtaatt
    16141 aaatatcaat gaagatttaa aaatagttaa attaaagtaa gagtctaata tataaaattt
    16201 atagtaactt tatggtttaa atataaactt gtgtgctatt gacaaagtat aaaattagca
    16261 gtaaaataat tgcatgagca aatagattat tgcgaacgca aaaaaatgag gacaatagat
    16321 attaaaggag agaatatgag agagctatta aaatgggttt ccattataaa tagatacaat
    16381 acaaattata ttgacaggaa attggcggat attggaatca ataatagcca atatttttat
    16441 gtattacaca tatgtaataa tccaggtatt acacaggaca ctatatttga gaaaattctt
    16501 gtgaatccta gtaatataac gagagctttg gcaaatttag aaaaagaagg ctttataaca
    16561 agagaaccag gtgtaaagga taaaagaaca tggcatttat atcctacaga aaaatcttta
    16621 gaatgttatg atgaaatact aaaaataaca aatgggtatg ttgaggagtt gttgagttca
    16681 tttgagaaag aagagcaaga gttgtttatg tcaatgttaa agaaagttgc atttttagct
    16741 attgatatga atgaagaagc aaaattggag gcgaaggaaa atggaagaaa aatataagga
    16801 gccattggaa tatgaaaagg tttcaaagtt attaactaag tacgcaattc ctagtattat
    16861 agcaatgtta gtaagtgctt tatataacat tgttgaccaa atatttattg ggcaaggtgt
    16921 tggtgtattg ggtaatgctg ctacaaatgt cacttttcca ctaacaacaa tatgtactgc
    16981 aattgcatta ttattaggaa ttggaggagc gtctaagtgt tcattggaat taggtgctaa
    17041 aaattcagaa aaagctacaa aagcagcagg aaattctata tgcttgatgg ctgtttttgg
    17101 aataagttta tttatagtag tatcaatatt tttaacacca ctattaaaat tctttggctc
    17161 tacatcagaa atactacctt atgcacacac atatacaaaa attacatcaa ttggattacc
    17221 atttcttata atgtccactg gaatgagtaa attgatactt gcagatggga gaccaaaagc
    17281 ttctatgttt tgtatgttaa taggagcagt tattaataca attttgaatc cattattcat
    17341 ttttgttttt aatatgggaa ttgctgggtc tgctttagca actgttattg ggcaacttat
    17401 atcttttgga ataagtgttc attatataaa gaattttcaa catattaaat tacataagaa
    17461 aagttttaaa ttagatagta taatttataa acatatattt gcgctaggtt caagctcatg
    17521 ttttaatcaa ttagcaatga ctgtaacaca gatagttatg aataatacat tggcatatta
    17581 tggggctagg tcagtttatg gaagtgagat tccacttgct tgtgtcggta tcattattaa
    17641 agtaaatatg atttttatat ctataattat tggtatatca caaggtatgc aaccaattat
    17701 agggtataat tatggggctg aaaagtataa tcgtgtaaaa gaagcatata agtttgcagt
    17761 gggaagtgca actattatat ctgttattgc attcttatgt ttccaattct tcccaagaca
    17821 aattacgtct atatttggta acggaaatga gacttatttt atatttgcag aacattattt
    17881 tagaactttc ttgttcttaa catttataaa tggtgttcaa atagtatcat caactttctt
    17941 tacatcaatt gggaaatcag tgttgggact ttttacttct ttgactcgtc aaattttatt
    18001 tttaattcca ttgatagtaa ttttaccaat gtttatggga attgatggag taatgtttgc
    18061 aggaccaata gcagatgcag cagcggcaat ggtatgtata tactttgcag ttcatgaaat
    18121 gaaatccttg actataaagc aaaataattt gatgagtaac cataataaga ttgcagaggt
    18181 ataagacaat atttaagaag tatagaaaat taatttaaga aatataataa actaatttag
    18241 gaagtgtaga aaattaa
//
LOCUS       DAMJWP010000058        17750 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.059, whole genome shotgun sequence.
ACCESSION   DAMJWP010000058 DAMJWP010000000
VERSION     DAMJWP010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17750
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.059"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            132..1937
                     /locus_tag="PC700_001679"
     CDS             132..1937
                     /locus_tag="PC700_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2580991.1"
                     /translation="MLSKLKEFQQEMIKYTETVASVLDVDIEIVDDRLIRISGTGLYK
                     SKINESVVTEGFIYDNVIQTGQELVVLDICDNQLCIECSHYMKCLNKVIIAVPIKYNN
                     RTIGVIGAISTDKTKKVEISAKIDNYLKFVNHICDLISMKIEEHEASKNSSRKMDMMI
                     EIIENVEKGVIILDINSKISYINNIALKKLDIYKNIIENIVNIVSVESSSNGHELLEI
                     DIDNKIYNINAKIIPVYPYINQYDKIIIFDKTYINHKGHGKVNSGWGNSDIESIIGNS
                     EAMLKVKERTKKLAKSNSTVLITGESGTGKELIARAIHAEGSRWNKPFIAINCAAIPE
                     NLLESELFGYIKGAFSGASSGGKVGKFELANEGVIFLDEIGDLSMPLQAKLLRVLQER
                     KFARIGSNKLIDLDIRVIAATNKNLLKLVNEGKFRDDLYYRLNVIPINLPPLRERKDD
                     IEAIMMKFASKYSLELGIQLNKIEENVMNMLINYNWPGNIRELENAVEYMMNLVGDDG
                     IIYKDMLPLDILNYYNINGNVCKNKDINIIFEDDIVGGIVENQERILSIKELELTYIN
                     KLLNKYGRDTKTKKKIAKDLGIGLATLYRKLEEEQ"
     gene            2014..2553
                     /locus_tag="PC700_001680"
     CDS             2014..2553
                     /locus_tag="PC700_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HDF2580992.1"
                     /translation="MQYAIELYYDKKTEKQLFDLSKKIADEKISTKYLEWKTRPHLTL
                     ACFNDVDEACCIDKLKGFAQNHKVIPAYIGSVGMFNDTKTVFVSPIMNSSMYQFQREL
                     YEVLQDFDSNGWEWYHPNRWVPHCTIALTGDDESEAFFRASDVLLREFKKISGEFVSI
                     GLVKITFPVEEIFTVNMNR"
     gene            3176..4390
                     /gene="dpaL"
                     /locus_tag="PC700_001681"
     CDS             3176..4390
                     /gene="dpaL"
                     /locus_tag="PC700_001681"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HDF2580993.1"
                     /translation="MREIKWKLNNLPKSEEKEKGIEFLRDEEIAKAKAFHESFPQYEK
                     TPLVKLDNLAKSLGVSGIYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAKEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNYSSAFVSAPDWVSAKGMRILGNP
                     LRGDEKVISGESGAVTTGALVSILESEDLKDLREALKLDENSKVLLISTEGDTDPDKY
                     RDIVWNGECQSK"
     gene            4723..5910
                     /locus_tag="PC700_001682"
     CDS             4723..5910
                     /locus_tag="PC700_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HDF2580994.1"
                     /translation="MLNETRKKQLTEVCQDLIRNASYSGQEENVVKAIEENFALLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEARIVDGKIYGRG
                     TSDMKGQVSAMMAAVSYFAEDTNKDFEGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKGINAVYKMANVIQKIQQLVPPT
                     HPVLGDGILVLTDIKSSPYPGASVVPDYCKATFDRRLLVGETREGVLAPIQALLDEMM
                     KEDPELNAKVSYAVEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYNGLKEAGIDSE
                     ITQYSFCTNGSHYAGEAGIRTIGFGPSKENLAHTIDEYIELDQLFKGAEGYYGILKSV
                     YKK"
     gene            5926..7509
                     /locus_tag="PC700_001683"
     CDS             5926..7509
                     /locus_tag="PC700_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HDF2580995.1"
                     /translation="MRTLIKHGLIVDGNKTPAYEGDILIENEKILKISQDLNEEADKV
                     IDAKGRVICPGFIDTHSHSDLVILVNPYNEVKIRQGITTEVLGQDGISMAPLPQEHIS
                     SWRKNLAGLDGESDEIDWKYETTENYLKMMDYNGVGLNETYLVPHGNVRMEAMGLEDR
                     PATKEEIQKMCEITERELKAGAIGLSTGLIYIPCAYSLTEEIIEMCKVVAKYDGVFVV
                     HQRSEADTILTSMEEIIEIGKQSGVKVHFSHFKVCGKANWKYIPQVIELLEKAEKEGI
                     RVSFDQYPYAAGSTMLGVVLPPWAHSGGTDKLIERLSDENERAKMKKDIANGIEGWDN
                     FIEFAGIDQIFVTSVKTEKNKDTIGKSLLEIGKMRGKDPLDATFDLLKEEENAVGMVD
                     FYGLEEHIIGFMKRDEQNVCTDGLLAGKPHPRAYGSFPKILGRYVRELNVLTIEEAVY
                     KMTKKAATSFSIKDRGELKEGYFADIVIFDKDTVSGCDDFINSMQYPTGIDYVIINGN
                     CVVEEGKYNKIKAGKVLKN"
     gene            7537..8865
                     /gene="ssnA"
                     /locus_tag="PC700_001684"
     CDS             7537..8865
                     /gene="ssnA"
                     /locus_tag="PC700_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HDF2580996.1"
                     /translation="MILVGNGRLISQDSLNPYMEDGCVVINDNLIEDIGTTEEMKAKY
                     PVHEFIDAGKKIIMPGLINSHMHIYSSFARGMAVPGKPSENFLEILNNLWWRLDKQLT
                     LEDTKYSAYATYIECIKNGVTTVYDHHASPNAIEGSLFTISDVAKDLGIRTCLCYEVS
                     DRDGGNTIQQGINENVNFIKYAQKDDTDMIKGMFGLHAAFTLSDDTLNKCASEMAGLD
                     TGYHVHVAEGLADLQYNVDKYGKRVLERFNDFGVLKEKTIAVHCIYISAEEISLLNKT
                     NTFVVHNPESNMGNAVGCSPAIELLKRGVTVGLGTDGYTSDMFESLKVANIIHKHHLK
                     DPRVGFEESQTMLFDNNRKIAKAYYKNDLGVLKKGAYADVIVVDYIPHTPLTENTIGG
                     HTIFGMSGRSVDTTIINGKVLMKDRKLINIDEESILAKSRELSQNLWNRI"
     gene            9064..10416
                     /locus_tag="PC700_001685"
     CDS             9064..10416
                     /locus_tag="PC700_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDF2580997.1"
                     /translation="MSQDKSPKIAPVDERLPVSKSWVFSLQHVLAMCAGAVAVPMMIG
                     SAARLDHASIVFLINACLFMAGIGTLIQSVGIGNIVGAKIPVIEGTSFAAVAGMTAIA
                     SSYSDSQVAIQTVFGSVIVAGLFCILLAPVFEKLIRFFPSVVTGTVVMVIGISLFPVG
                     IKWITDNKLEAVEPNSLALALAVLAITLLSFKFLKGIWNSTAILVGIVLGTLLSMAVG
                     MADFSAVNGVGWLNLNVPLKFGMPRFEPSAILSLCLIMLVLMTESVGNMIAIHEMVDK
                     EVTGKNIKRGLFGDGISTALSGIFNSFPITPFGQNTGIVGLTGIKSRFIAVYAGVILL
                     VLGFLPKFAAIIGAIPKPVLGGVGFAMFGMVMVGGVKTLSKVKFDGTKNGVIVAVSVG
                     LSMIPLTNPDFYHNFPTWVQTIFHSGITTGSLSAVLLNIFFNELGSKKEKNNKVEEAK
                     "
     gene            10608..11717
                     /locus_tag="PC700_001686"
     CDS             10608..11717
                     /locus_tag="PC700_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2580998.1"
                     /translation="MANLKVNLYGMEFLNPIMTAAGPGAKDGALCQEAAKGGAGAICT
                     KTISVLPADVPRPCMAKTNSGFLNTELWSELPKEQWIEEEYKKVKETGLPVIVSMGYS
                     AEQIAEVAPLVKPYADAVELSTHYVGTDVAPIVNALKAAKAVLDVPVFMKMSPHTDIQ
                     TIAKAVEEAGADGLVMINSFGPCMAIDVNTGYPIMGSKAGYGWLSGAPIRPLAIRCIY
                     EASQVVNIPIIGVGGITCGKDVAEMMMAGASAVQVCTEAILKGPSIYGKIAKELNEFL
                     DENGYADVNEIKGLTHKKMAARGFRTHSVAPVVDDNKCIKCKQCVTSCVYEALEVTDK
                     LNIDADKCFGCGLCVTRCPKGALSICYNQVEAMNE"
     gene            12000..13391
                     /gene="hydA"
                     /locus_tag="PC700_001687"
     CDS             12000..13391
                     /gene="hydA"
                     /locus_tag="PC700_001687"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HDF2580999.1"
                     /translation="MGIVLKGGKVVTSEKTYCADVRIEDEKIVDIGLDISKENDEIVD
                     VSDSYVIPGGIDTHTHFDLDTGVTKTADNFETGTKAAIVGGTTTILDFATQAKGCSLK
                     DGLEEWHEKAKDKSYSDYGFHMAITDWNDSVCNEMEDMVKEGVSSFKLYMAYKGSLQV
                     DDGVIFEALRKAEEIGGIIGFHCENGDIICELVDKAKSENNLSPKYHQLTRPAIMEAE
                     ATSRLMKIAEVANSKVYVVHLSCKESLEEVLKAKQRGVDVIVETCPQYLLLNDELYDL
                     DGFESAKYVMSPPLRNKENNEILWNAILDNQIDTIGTDHCSFNYKGQKELGINDFSKI
                     PNGSPGVEHRMSLLYTYGVCKNKISINKMVEVTSTNAAKLFGMYPNKGTIEIGSDADI
                     LVLNPNKKSKIEAKKQLQNVDYTPYEGYEVDCQIEHVYLRGKEVVRDGELICNTPSGR
                     YINRRLTKGAREW"
     gene            13385..15946
                     /gene="xdh"
                     /locus_tag="PC700_001688"
     CDS             13385..15946
                     /gene="xdh"
                     /locus_tag="PC700_001688"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HDF2581000.1"
                     /translation="MVEFILNGEKVVAKEGKKLLDFLREDMNITSVKNGCSEGACGTC
                     MVIIDGKATKACVFKTDKLAGKNITTIEGLSERERDVFAYSFASQGAVQCGFCIPGMV
                     ISSKALIDVNNNPTEDDIKKALKGNICRCTGYVKIIKAVDLAAKIFRENIDVPKVNCK
                     GLVGENLPRVDAVGKALGYSEYVDDMRIEGMIYGSAVRAKYPRALVKKIDIEEAKAYP
                     GVVAVLTAKDIPGTIKVGHLKKDWDTLIPEGEITRYLGDGIVLVAAETREALEEAKKL
                     VKIDYEELTPLSTTADALREDAPKIHETGNILVKEHLVRGNAKEKIENSKYVVTKRYT
                     TPATEHAFLEPECAVAGPYEDDGVIIYSTDQGVFATQHECADMLGLPYEKVRVINKMV
                     GGGFGGKEDMSVQHHAALLAYHTKRFVKVQLTRKESIIVHPKRHATEMEFTTACDENG
                     YLTGMKATIISDTGAYASLGGPVLQRLCTHAAGPYNYQDIDITGTAVYTNNPPGGAFR
                     GFGVTQSCFATECNLNLLAEMVGISPWEIRYKNAIRPGQVLPNGQFADEGTAMAETLE
                     AVKEEYEKHPRAGIACAMKNSGVGVGIPDVGRCRLVIKDGKVHIRTSASCIGQGLGTV
                     MTQVVCETASLLPENIVHEVPDTHLTPDSGNTTASRQTVFTGEATKVASNKLKEALDS
                     GKTLLDLEGEEFYGEYESITDKMGCDKENPVSHVAYGYATQVVILDENGKLEKVVAAH
                     DVGRAINPISLEGQIEGGVVMGLGYALTEDYPVVNSVPTAKFGTLGLFKSTDIPEIQA
                     LIVEKNKDLLAYGAKGVGEICTVPTAPAVQNAYYVFDKEFRTELPLQNTPYSKKK"
     gene            16098..17444
                     /gene="rpoN"
                     /locus_tag="PC700_001689"
     CDS             16098..17444
                     /gene="rpoN"
                     /locus_tag="PC700_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HDF2581001.1"
                     /translation="MNFNNSLELTQSQKLIMTTQLKQSLSILNMSKLELEEEIKREAE
                     NNPLVEVEKSGEINWEEYIKDMDRSRRLDRTEISYNPDNEVNLENLVKYSSNLYEDLK
                     FQISLYKLTDKELEVCEYIIDSLDEDGYLRTEEKVIVDTLKIDEELFEKCLISVQQLE
                     PSGVGARSLSECLMIQMASLGIYNEILEEIVTKDLNLIANNKYKEISKKYNMSLQKCV
                     ELINIIKTLDPKPGRTCSVEKSVYIQPDVTVEKIDDEFIVYLNEKDSYQIRINSYYKD
                     ILKNSQSDESAKEFIKERLNSATGLMKNIESRKTTVLKIAEEIVKAQDEFLRKGTKYI
                     KPLKMKDIAEKLEFHESTISRGVNGKYMLTPFGVYEFRYFFSSAIETENNEMASSTSI
                     KKIIKETIKDENKKKPLSDDHISKLLKEKGINVARRTVAKYREELGIPSSSKRKEY"
BASE COUNT         7124 a         1997 c         3560 g         5069 t
ORIGIN      
        1 taaattaata ttgtgtgttt taatgtaaat atataaataa gtaatgtttt atatatattt
       61 taaagagtga ctatatgtta aaatatagct aaactgataa tttggtaaat taaattctaa
      121 tgggggaaac tatgttaagt aaattaaaag aatttcaaca agaaatgata aaatacactg
      181 aaacagtagc tagtgtgtta gatgtagata ttgaaatagt tgatgataga ttaataagaa
      241 ttagtggtac aggtctgtat aaatcaaaga taaatgaaag cgttgttaca gaaggattta
      301 tttacgacaa tgttatacag acaggtcagg aattagtcgt attggatata tgtgataatc
      361 agctttgtat agaatgttcc cattacatga aatgcctaaa taaagtaata atagcagttc
      421 caataaagta taataatcga actataggag taattggtgc tattagtaca gataaaacta
      481 aaaaagtaga aataagtgca aaaatagata attatttaaa atttgtcaat catatatgcg
      541 atttaatttc tatgaaaata gaagaacatg aagcgtcaaa aaatagcagt agaaaaatgg
      601 atatgatgat agaaatcatt gaaaatgttg aaaaaggcgt tataatacta gacataaata
      661 gcaaaatatc ttatataaac aatatagctc taaaaaaatt agatatatat aaaaatataa
      721 tagaaaatat agtaaatata gtatctgttg aatcttcctc aaatggacat gaattactag
      781 aaattgatat tgataacaaa atttataata taaatgccaa aataattccg gtatatccat
      841 atataaatca atacgacaaa ataataatat ttgacaagac atatatcaat cataaaggac
      901 atggaaaagt caatagtggc tggggaaata gtgatataga gtctataatt ggaaattcag
      961 aggcaatgtt aaaagtaaaa gagagaacta agaaattagc aaagtcaaat tcaactgtac
     1021 ttataactgg ggaaagtggt acgggtaaag agctaattgc tagggctata catgctgaag
     1081 gtagcagatg gaacaagcca tttattgcga taaactgtgc tgctattcca gaaaatctcc
     1141 ttgaaagtga gctatttggc tatataaaag gtgcatttag tggagctagt agtggaggta
     1201 aagtaggtaa atttgaactt gcaaatgaag gtgttatatt tcttgatgaa ataggagatt
     1261 tgtcaatgcc tttacaagca aagttactta gagttttaca agagagaaaa tttgcgagaa
     1321 ttgggtcaaa taaacttata gatttagata taagagtgat tgctgctaca aataaaaact
     1381 tattgaaatt agtaaatgaa ggtaagttta gggatgattt atactataga ttaaatgtaa
     1441 ttcctataaa tttgccacct cttagggaaa gaaaggatga tattgaggca ataatgatga
     1501 agtttgcttc taaatattct ttagaattag gtattcagtt aaataaaata gaagaaaatg
     1561 taatgaatat gttgattaac tataattggc cgggaaatat aagggaactg gaaaatgcag
     1621 ttgaatatat gatgaatttg gttggagatg atggaataat atataaagat atgcttccat
     1681 tggatatttt aaattattat aatattaatg gaaatgtttg taaaaataag gatataaata
     1741 taatatttga agatgacata gttggaggga tagtagagaa tcaggaaagg atactttcaa
     1801 ttaaagaatt agagttgact tacataaaca agttgttgaa taagtatgga agagatacaa
     1861 agacgaagaa gaagatagca aaggatttag gtataggttt ggctacacta tatagaaaat
     1921 tggaagagga acagtagtaa tataatatta aaatgatagg tatttgtgtg tttttataga
     1981 taggtatttt tactttatgg tggaggtgtt attatgcaat atgcgataga gctatattat
     2041 gataaaaaaa cggaaaagca actttttgat ttatctaaaa aaatcgctga tgaaaaaatt
     2101 agtacgaagt acttagaatg gaaaacaaga cctcatctta ctttggcttg tttcaatgat
     2161 gtggatgagg cttgttgcat agacaagcta aaggggtttg cacaaaacca taaggtaata
     2221 cctgcttaca ttggttcagt aggcatgttt aatgatacta aaacggtgtt tgtatcacct
     2281 ataatgaata gcagtatgta tcagtttcag agagaattat atgaggtact acaagatttt
     2341 gattcaaatg gttgggaatg gtaccacccc aatcgttggg taccccattg cacaatagca
     2401 cttactggag atgatgaaag tgaggctttt tttcgggcaa gtgatgtatt actgcgtgag
     2461 ttcaaaaaaa taagtggtga gtttgtctca ataggattgg ttaaaattac atttcctgtg
     2521 gaagaaattt ttacagtgaa tatgaatagg taactttaac tctgttgtgg atgttatgaa
     2581 tattttgcat agtgcatttc atcagataaa tgcgaagttg cgaatttcga cagctcaata
     2641 gtcgcaagta ggacacaatg gtacacatat tgcacttttg cttattggag ggacgcccat
     2701 gtaaacttct gtaagaagct atgaaaagat ttaagccatt ttggaggctt tgggtaataa
     2761 agaatatttg ggtaataaag aatataaagt atataaattt atagagttta tgcgtaagaa
     2821 ggttatagcc atatatttat agaaaataaa agtagtaata tatctgacaa aacaatagac
     2881 atataaaaaa taagagcata ataaaggaag aattaaaata aataaacgat ttctcgaatt
     2941 gataattctc gtattgataa aaatttccca atatgagaat tttttctttt ataaatctta
     3001 tttttcacat ttaatattta taaaattcaa agaaatttta taaatatatg taattccaac
     3061 ataaaaaaat tgtttttaga ataaataata ttggcatagt aattgcttca ctataagaca
     3121 caaagataaa aaattaaaat gacaccgaaa aatttgaaag gggaatataa aataaatgag
     3181 agaaataaaa tggaaattaa ataacttacc taaatcagag gaaaaagaaa aaggaattga
     3241 gtttttaaga gatgaagaaa ttgcaaaggc taaagctttt catgagagct ttccacaata
     3301 tgaaaaaact cctttagtta aattagataa cttagcaaaa tcactaggcg taagcggaat
     3361 atatgttaaa gacgaatctt atagatttgg tttaaacgca tttaaagttt taggtggttc
     3421 ttactctatg ggtagatact tagctcaaag attagataca gatatatctg agttagggta
     3481 tgacaaatta acttcaaaag aaataaaaga aaaattagga gaaataactt tctttacagc
     3541 cacagatgga aatcatggta gaggagttgc ttggacagct aataaattag gtcaaaaatc
     3601 tgttgtatta atgccaaaag gttcttctga atttagatta aataaaatca aaggagaagg
     3661 tgcagatgct agtataacag acttaaacta tgatgatgca gtaagattag ctaatgatta
     3721 tgcagaagct gatgaccatg gagtaatggt tcaagataca gcatgggacg gatatgaaga
     3781 aataccagca tggataatgc aaggatatgg aactatggct aaagaagcta tagaacaatt
     3841 aaaagaatat ggagtagata gaccaacaca cgtatttgtt caagcaggag ttggttcact
     3901 tgctggtgca gttcaagggt atgttgcttc aatatatgat gaatgcccaa taacagtagt
     3961 agtagaggct gatgaggctg attgttacta taaatcagca gaagctggag atggaaaacc
     4021 aagatttgtt ggaggagata tgccaactat aatggcaggg cttgcttgtg gagaaccaaa
     4081 cactataggt tttgaagtgt taaaaaatta ttcatcagca ttcgtttcag caccagactg
     4141 ggtatctgct aaaggtatga gaatattagg aaaccctcta agaggagatg agaaagtaat
     4201 ctctggagaa tctggagcag ttacaactgg agcattagtt tcaatccttg aaagtgaaga
     4261 tttaaaagac ttaagagaag ctttgaaact agatgagaac tctaaagtat tattaataag
     4321 tacagaagga gatactgacc ctgataaata tagagatata gtttggaatg gtgaatgcca
     4381 aagtaaataa aagacttaag gaaaataaaa acttaacaaa taaatgaaaa atttaaggaa
     4441 aataaaaact taaataagtg aaaactttaa ataaaataaa aactaaaaca aatgaaaaat
     4501 ctaaataaat gaaaaagtaa agcaaatgaa aaattcaaaa taaaaactaa ataaaattaa
     4561 ataaaaccaa ataaaattaa aataaagtct aaataaaaat ttaataaata aaatataaag
     4621 aaataaaaaa tcaatagatt aagtgaaatg ctaataaatt aaaaaagtga aatatcaaaa
     4681 taaaacgtca acaaattaaa aattatatgg gggaatatca aaatgttaaa tgaaacaaga
     4741 aaaaaacaat taactgaagt atgccaagac ttaataagaa atgctagtta ttcaggtcaa
     4801 gaagaaaacg tagtaaaagc tatagaagaa aattttgctt tactaggttt tgatagttgg
     4861 tcaagagata gatatggaaa tatagtagga tgtataaaag gaaataaacc aggtaaaaaa
     4921 atattatttg acgggcatat agatacagtt ccagttccag atgcaagtaa atggagtgtt
     4981 cctccttttg aagcgagaat agtagatggg aaaatatatg gtagaggaac ttctgatatg
     5041 aaggggcaag taagtgctat gatggctgct gtttcatact ttgcagaaga tactaacaaa
     5101 gactttgaag gtgaattgta tgtagcaggt gttgttcatg aagaaatatt tgaaggtgtt
     5161 tcagctagag agataagtaa ggctgttcaa cctgattatg ttgtaatagg tgaatcttca
     5221 gaattaaact taaagatagg tcaaagaggt agaggagaaa tagtagtaga aacttttggt
     5281 aaaccagctc actctgctaa tccagaaaaa ggtataaatg cagtttataa aatggctaat
     5341 gtaatccaaa agatacaaca attagtacct ccaactcatc cagttttagg tgatggaata
     5401 ttagttttaa cagatataaa atcttctcca tatccaggag catcagtagt gccagattat
     5461 tgcaaagcta cttttgatag aagattatta gttggagaaa caagagaagg cgtattagct
     5521 ccaatacaag ctttattaga tgaaatgatg aaagaagacc cagaattaaa tgcaaaagtt
     5581 tcttatgcag ttgaaaaagc tgattgctat acaggaaata ctatagaaag tgaaagattc
     5641 ttcccaggtt ggttatatga tgaagaagat gaatttgttc aagcagcgta taatggatta
     5701 aaagaagctg gaatagattc agaaataact caatactcat tctgtacgaa tggaagtcac
     5761 tacgcaggtg aggctggaat aagaactata ggatttggtc cttctaagga aaatcttgct
     5821 catacaatag atgagtatat agaattagac caattattca aaggtgcaga aggatattat
     5881 ggtatattaa aatcagttta taaaaaataa ttaggagatg gttttgtgag aactttaata
     5941 aaacatgggc ttatagtaga tggaaataaa acaccagcat atgaaggaga tattttaata
     6001 gaaaacgaga aaatattaaa aatatcacaa gatttaaacg aagaagctga caaagttata
     6061 gatgcaaaag gtagagttat atgtccaggt ttcatagata ctcatagcca ttcagaccta
     6121 gttattttag ttaatccata taatgaggta aagattagac agggaattac aacagaagtg
     6181 ttgggccaag atggtatatc tatggcccct ctacctcaag agcatatatc ttcttggaga
     6241 aaaaatttag caggtctaga tggtgaaagt gatgaaattg attggaaata tgaaacaaca
     6301 gagaattatt taaaaatgat ggattacaat ggggtaggtt taaatgaaac ttatttagta
     6361 cctcatggaa acgttagaat ggaagctatg ggacttgaag atagacctgc tactaaagaa
     6421 gaaattcaaa aaatgtgtga aatcacagag agagaattaa aagcaggagc tataggattg
     6481 tctactggac ttatatatat accttgtgca tactcattga ctgaagaaat tatagagatg
     6541 tgtaaggtag tagcaaaata tgacggagta tttgtagttc accaaagaag tgaagcagat
     6601 acaattttaa cttcaatgga agaaatcata gaaattggaa aacaatcagg agtaaaagtt
     6661 catttttcac attttaaggt ttgtggtaaa gcaaactgga agtatatacc acaagttata
     6721 gagcttttag aaaaagcaga aaaggaagga ataagagtat cttttgacca atatccatat
     6781 gcagcaggaa gtacaatgct tggagtagtt ttacctcctt gggcacattc tggaggaact
     6841 gataagttaa tagaaagatt aagcgatgaa aacgaaagag caaaaatgaa aaaagatata
     6901 gcaaatggaa tagaaggctg ggataatttt atagaatttg ctggaataga ccaaatattt
     6961 gtaacaagcg taaaaacaga aaagaataaa gatacaattg gtaagagctt attagaaatt
     7021 ggaaaaatga gaggaaaaga cccattagat gcaacgtttg atttattaaa agaagaagaa
     7081 aatgcagttg ggatggtaga tttctatgga ttagaagaac atataatagg attcatgaaa
     7141 agagacgagc aaaatgtgtg tacagatggt cttttagcag gtaaaccaca tccaagagct
     7201 tatggttctt tccctaaaat actaggaaga tatgttagag agttgaatgt actaacaatt
     7261 gaagaagctg tttataaaat gactaaaaag gcagctacaa gctttagtat aaaagataga
     7321 ggcgaattaa aagaaggata ttttgcagat atagttatat ttgacaagga tacagttagt
     7381 ggttgtgatg attttatcaa ctctatgcag taccctacag gtatagatta cgtaattata
     7441 aatggaaatt gtgttgttga agaaggaaaa tacaataaaa ttaaagcagg aaaagtactt
     7501 aaaaattaaa tcttagcttt aaggaggaat aaatttatga ttttagtggg gaatgggagg
     7561 ctaataagcc aagattcttt aaatccctat atggaagatg gatgtgtagt aataaatgat
     7621 aatctaattg aagatatagg tactacagaa gaaatgaaag ctaaatatcc agtacatgaa
     7681 tttatagatg caggcaagaa aataatcatg ccgggtctaa taaattcaca tatgcatata
     7741 tacagctcat ttgctagagg aatggcagtt ccaggaaaac cttctgagaa ctttttagag
     7801 atactaaata atttatggtg gagacttgac aaacagctta cactggaaga tacaaaatac
     7861 agtgcatatg caacgtatat agaatgcata aaaaatggtg taacaacagt ttatgaccat
     7921 catgcaagtc caaatgcaat tgaaggcagt ttgtttacaa tatcagatgt tgcaaaggat
     7981 ttgggaatta gaacgtgtct ttgttatgaa gtgtctgata gagatggtgg aaatacaatc
     8041 caacaaggta tcaatgaaaa cgtaaacttt ataaaatacg ctcaaaaaga tgatacagac
     8101 atgataaaag gaatgtttgg gcttcatgct gcatttacac tatctgatga tacattaaat
     8161 aaatgtgctt cagagatggc tggtcttgat acaggttacc atgtacatgt tgctgaagga
     8221 ctagcagact tacaatataa tgtagataaa tatggaaaaa gggttttaga aagattcaat
     8281 gattttggag tactaaaaga aaaaacaata gcagttcatt gtatatatat aagtgcagaa
     8341 gaaatcagtc ttttaaataa aacaaataca tttgtagttc ataatccaga atctaatatg
     8401 ggaaatgcag ttggatgttc tcctgcaata gagttattaa aaaggggagt tacagtagga
     8461 ttaggaactg atggatatac tagcgatatg tttgaatcat taaaagttgc caatataatt
     8521 cacaaacatc acttaaaaga cccaagagtt ggatttgaag aatcacaaac aatgttattt
     8581 gataacaata gaaaaatagc taaagcttat tataaaaatg acctaggagt acttaaaaaa
     8641 ggtgcttatg cagatgttat agtagttgat tatataccac atactccatt gacagaaaat
     8701 acaatagggg gacacactat ttttggaatg agtggaagaa gcgtagatac aactattata
     8761 aatggaaaag tacttatgaa agatagaaag ttaataaaca tagatgaaga aagtattcta
     8821 gcaaaaagta gagaattaag tcaaaatcta tggaatagaa tttaaataat tgattatgca
     8881 aaattctatg aagcaggaac cctgtgactt tagtcatggg aggttcagat taatatacaa
     8941 gtaagaaaca agaatttaga tgttaaaaac agagcttaaa caaaaatagg aatttaaatg
     9001 aagtatataa ataaaaaata tacggaatag aatattaaat taaatataga agggagaccc
     9061 aaaatgtctc aagataaaag ccctaaaata gctccagtag atgaaaggtt acctgtatcc
     9121 aaatcttggg tattttcact acagcacgtt ttagcaatgt gtgcaggagc agtagcagtt
     9181 cctatgatga taggaagtgc tgcacgacta gatcatgcta gtattgtttt tttaataaat
     9241 gcttgcttgt ttatggcagg tataggaaca ctcatacaaa gtgttggtat aggaaatatt
     9301 gtaggtgcta aaataccagt aatagaagga actagttttg ccgctgtggc aggtatgaca
     9361 gcaatagcct catcatatag tgattcacaa gttgcaatac agacagtatt tggctctgta
     9421 atagtagctg gattgttttg tatattactt gctccagtat ttgaaaaatt aataagattt
     9481 ttccctagtg tagttactgg aacagttgtt atggtaatag gtatttcact atttccagtt
     9541 gggataaaat ggataactga caacaaattg gaagcggttg aaccaaactc attagcatta
     9601 gctttagcag tattggcaat aactttactt tcatttaaat tcttaaaagg aatttggaat
     9661 agcacagcta tattggtagg tatagtactt ggtacactac tttccatggc agttggaatg
     9721 gcagatttta gtgcagtaaa tggagttggt tggcttaact taaatgtacc tttaaaattt
     9781 ggaatgccta gatttgaacc atctgcaata ctatcattgt gtttgattat gttggtactt
     9841 atgacggaat cagttggtaa tatgatagct attcatgaaa tggtagacaa agaagtaaca
     9901 ggtaaaaata ttaaaagagg tctatttgga gatggaatat ctacagcatt atctggaata
     9961 ttcaattcat ttccaataac tccatttggt caaaatacag gaatagtagg tcttacaggt
    10021 ataaaatcaa gatttatagc agtttacgca ggggttatat tgctagtact tggattctta
    10081 ccaaaatttg cagcaataat aggagcaata cctaagcctg ttctcggagg cgtaggattt
    10141 gcaatgtttg gaatggtaat ggttggagga gtaaaaactt taagcaaagt gaaatttgat
    10201 ggaactaaaa atggggttat agtagcagtt agtgttggtt tgtctatgat accactaaca
    10261 aatcccgatt tctatcataa tttcccaact tgggtacaaa caatattcca tagtggaata
    10321 acaacaggta gtttatcagc agtgctttta aacatattct tcaatgaact aggttctaaa
    10381 aaagaaaaaa ataataaagt tgaagaagca aaataaaaat aaaacaagta aaaatctata
    10441 ttgacttaaa gtcattaagc attaataaaa ataattcaga gatattaata attcaacaaa
    10501 actatcttat attttaaata atataaaaaa gtaaataaat aacgaataca aataaaagaa
    10561 tacaatctaa atattaaaaa tatttaattt aagggagaga taataatatg gctaatttaa
    10621 aagttaattt atatggaatg gaatttttaa acccaattat gacagcggca ggacctggag
    10681 caaaagatgg tgctctctgt caagaggcag caaaaggtgg agcaggagct atatgtacaa
    10741 aaactatatc tgtattacca gctgatgtac caagaccatg tatggcaaaa actaatagtg
    10801 gttttttaaa cacagaatta tggtcagagc ttccaaaaga gcaatggata gaggaagaat
    10861 acaaaaaggt aaaagaaaca gggcttccag taatagtaag tatgggatat tcagcagagc
    10921 aaatagctga ggttgctcct ttagtaaagc cttatgcaga tgcagttgaa ttatctactc
    10981 attatgtagg tacagacgtt gcaccaatag taaatgcttt aaaagctgca aaagctgttt
    11041 tagatgtacc agtatttatg aaaatgagtc ctcatactga cattcaaact attgcaaaag
    11101 ctgttgaaga agcaggtgca gatggacttg taatgataaa ctcatttgga ccatgtatgg
    11161 caatagatgt aaatacagga taccctataa tgggaagtaa agctggatac ggatggttat
    11221 caggtgcacc aataagacct ctagcaataa gatgtatata tgaagcttct caagttgtaa
    11281 atataccaat aatcggtgtt ggtggaatca cttgtggaaa agacgtggct gaaatgatga
    11341 tggctggagc aagtgctgtt caagtatgta cagaagcaat ccttaaaggg ccttctatct
    11401 atggaaaaat tgctaaagaa ttaaatgaat ttttagatga gaatggatat gctgatgtta
    11461 atgaaataaa aggattaact cataagaaaa tggcagctag agggtttaga actcattcag
    11521 tagctccagt agtggatgat aacaagtgca tcaaatgtaa gcaatgtgta acaagctgtg
    11581 tatacgaagc tttagaagtt acagataagt tgaacataga tgcagacaag tgttttggat
    11641 gtggattatg tgtaactaga tgtccaaaag gagctttaag catctgctac aatcaagtag
    11701 aagctatgaa tgagtaataa ttatatagaa ttttggtaat taacttagag taataggtat
    11761 taggataata aaatatatca gataaacaaa cacacattat gcattaataa tacagtctaa
    11821 agttaataat tttattatat tgatttaact aaatattagt ttagctgaat attaatttaa
    11881 ttaggtattg gtttaatctt aatatagatt tactacgtat aagtgaacta actagattac
    11941 tttaagttaa ttttttcata taaaataaat tgatacatta caagtaaggt ggagtctata
    12001 tgggaattgt tttaaaaggt gggaaagtag taacaagtga aaaaacttac tgtgcagatg
    12061 taagaataga agatgaaaaa atagtcgata taggtcttga tatatcaaaa gaaaatgatg
    12121 aaatagttga tgttagtgat tcttatgtta taccaggagg tatagataca cacacacact
    12181 ttgacttaga tacaggcgtt actaagacag cagataactt tgagacagga acaaaagctg
    12241 ctatagtagg aggaactact actatattgg attttgctac acaagctaaa ggatgttctt
    12301 tgaaagacgg attagaagaa tggcatgaaa aagctaagga taaatcctat tcagattatg
    12361 gctttcatat ggctataaca gattggaatg actcggtttg taatgaaatg gaggatatgg
    12421 tgaaagaggg agtatcttca tttaaactgt atatggcgta taaaggaagc cttcaagttg
    12481 atgatggtgt aatctttgaa gcattgagaa aagcagaaga gataggaggg attattggtt
    12541 tccattgtga aaatggggac ataatatgtg aacttgtgga taaagcaaag tcagaaaata
    12601 atttatctcc aaaatatcat caattaacta gaccagctat aatggaggca gaagctacat
    12661 caagacttat gaagatagct gaagttgcaa actcaaaagt atatgtagtt cacttaagct
    12721 gtaaagaatc cttagaggag gttttaaaag ctaagcaaag aggtgtggat gtaatagttg
    12781 aaacttgtcc tcaatacctt ctattaaatg atgaattata tgatttagat ggatttgaaa
    12841 gtgctaagta tgtaatgtca ccacctttaa gaaataaaga aaataatgaa atattatgga
    12901 atgctatttt agataatcaa atagacacta taggaacaga ccattgttct tttaattata
    12961 agggacagaa ggaacttggg ataaacgatt tcagtaagat accaaatggt tctccaggtg
    13021 tagaacatag gatgagtctt ttatacacat atggagtctg taaaaataaa atatctatca
    13081 acaagatggt agaagtaaca tcaactaatg cagcaaagtt gtttggaatg tatcctaata
    13141 aaggaactat agaaataggc agtgatgcag atatattagt cttaaatcca aacaaaaaat
    13201 ccaaaataga agcaaaaaaa caattacaaa atgtagatta tacaccttat gaagggtatg
    13261 aagtggattg tcaaatagaa catgtttatt taagaggaaa agaagtagtt agagatggag
    13321 aattaatttg taatactcca agtgggagat atataaatag gcgtctaaca aagggggcta
    13381 gagaatggta gaatttatat taaatggaga gaaagttgtt gcaaaagaag gcaaaaaact
    13441 tttagacttc cttagagaag atatgaatat aacttcagtt aaaaatggct gttcagaagg
    13501 tgcttgtgga acatgtatgg ttataataga tggtaaagct actaaagcat gtgtatttaa
    13561 aactgataaa ttagctggga aaaacataac aactatagag ggattatcag agagagaaag
    13621 agatgttttt gcatattctt ttgctagtca aggagctgta caatgtggat tttgtattcc
    13681 aggtatggta attagttcaa aagcacttat agatgttaat aataatccta ctgaagatga
    13741 tataaagaaa gctttaaaag gtaatatatg tagatgtaca gggtatgtaa aaataattaa
    13801 agctgtcgat ttagcagcaa agatttttag agaaaatata gatgtgccaa aagtaaactg
    13861 taagggtcta gtaggtgaaa atcttcctag agttgatgca gttggcaaag cacttggata
    13921 ttctgaatat gtagatgata tgagaattga gggtatgatt tatggttcag cagttagagc
    13981 taagtatcct agagctttag ttaaaaaaat agatatagaa gaagcaaaag cttatccagg
    14041 agttgttgca gttctaacag ctaaagatat tccaggaact ataaaggtag ggcacttaaa
    14101 gaaggattgg gatactttaa taccagaagg tgaaataact agatatttag gagatggaat
    14161 agttttagta gcagcagaaa ctagagaagc gttagaagag gccaaaaagc tagttaagat
    14221 agattatgag gaattaacac cattaagtac tacagcagat gcacttagag aagatgctcc
    14281 aaagatacat gagacaggga atatacttgt taaggaacat ttagtaagag gaaacgctaa
    14341 agaaaagata gaaaattcaa aatatgttgt aactaaaaga tatactacac ctgctacaga
    14401 acacgcattt ttagagcctg aatgtgcagt agctggtcct tatgaagatg atggagtaat
    14461 aatctattca acagaccaag gggtgtttgc cactcaacat gaatgtgcag atatgttagg
    14521 gttaccatat gagaaggtaa gagttataaa caaaatggta ggaggagggt ttggtggaaa
    14581 agaagacatg agtgttcaac accatgcagc cttacttgca taccatacaa agagatttgt
    14641 aaaagtacag ttaacaagaa aagaaagtat tattgttcat ccaaaacgac atgccacaga
    14701 aatggaattt acgacagcat gtgatgaaaa tggatactta acgggtatga aggcaacgat
    14761 aatatctgac acaggtgcat atgcctctct tggaggacca gtgctacaaa gactgtgtac
    14821 tcatgcagca ggaccataca attatcaaga tattgatata acaggaacag cagtatatac
    14881 taataaccct cctggaggag catttagagg atttggagtt acacagagtt gttttgcaac
    14941 tgaatgtaat ctaaatttac ttgctgaaat ggttggaata tctccttggg agataagata
    15001 taaaaatgca ataagacctg ggcaagtttt accaaatggt caatttgcag atgaaggaac
    15061 agcaatggca gagacactag aagctgtaaa agaagagtat gaaaagcatc cacgtgcagg
    15121 tatagcttgt gctatgaaaa atagtggggt tggagttggt atacctgatg ttggaagatg
    15181 tagattggtg ataaaagatg gcaaagttca cataagaaca agtgcttctt gtataggtca
    15241 agggcttggt actgttatga ctcaagtagt ttgtgaaact gctagcctac ttcctgaaaa
    15301 tatagtgcat gaagttccag atactcattt aacaccagac tctggtaata ctacggcttc
    15361 aagacagaca gtatttacgg gagaagctac taaggttgca tcaaataagt taaaagaagc
    15421 tttagactct ggcaaaactt tacttgattt agagggagaa gagttttatg gagaatatga
    15481 aagtattaca gataaaatgg gttgtgacaa agaaaatcca gtaagtcatg ttgcttatgg
    15541 atatgcaaca caagttgtaa ttctggatga aaatggaaaa ctagaaaaag tagtagcagc
    15601 acatgatgtt ggaagagcta taaatccaat cagtttagaa ggtcaaatag aaggtggagt
    15661 agttatgggt cttggatatg ctctaaccga agattaccca gtagttaact ctgttcctac
    15721 agctaagttt ggtacattag gtttatttaa atctacagat ataccagaaa tacaagcttt
    15781 aatagttgaa aagaacaaag atttattggc ttatggggca aaaggtgtag gagaaatttg
    15841 tacagttcct actgcaccag cagtacaaaa tgcctattat gtatttgata aagaatttag
    15901 aactgagcta ccactacaaa atactccata tagtaaaaaa aagtaacaaa aaaaagaaat
    15961 attaaataaa aaaatagatg aaattgtaaa taaaaaaaac ccttttgcat aaggtatatt
    16021 ttaaatgata gaatatacat atacaggagg gtttttcttt tataaaataa gtttaataaa
    16081 attgagaggt gagcagaatg aattttaata atagtctaga attaactcag tctcagaagc
    16141 taataatgac tacacaactt aagcaatcat tatcaatatt aaatatgagt aagttagagt
    16201 tagaagaaga gataaaaaga gaagcagaga ataacccatt agttgaagtc gagaaaagtg
    16261 gggagataaa ttgggaagag tatataaaag acatggatag gtcaagacgt ttagatagaa
    16321 cagagataag ctataatcct gataatgaag ttaatctaga aaatttagtt aaatattcgt
    16381 caaatttata tgaagattta aaatttcaga taagcctgta taaacttaca gacaaagaat
    16441 tggaagtctg tgagtatata attgatagtt tagatgagga tggatattta agaactgagg
    16501 aaaaagtaat tgttgatacc ttaaagatag atgaagaact ttttgaaaaa tgtttaatta
    16561 gtgttcagca gttggaacct agtggagtag gggcaagaag tctttcagaa tgtctgatga
    16621 tacaaatggc tagtttagga atatataatg aaattttaga ggaaatcgtt acaaaagact
    16681 taaatttaat tgcaaataat aaatataaag agataagtaa aaagtacaat atgtcattac
    16741 aaaaatgtgt tgaactaata aacataatta agacattaga ccccaaacca ggaagaactt
    16801 gttcagtaga gaaaagtgta tacattcaac ctgatgtaac agtagaaaag attgatgatg
    16861 aatttattgt atatttaaat gagaaagatt cttaccaaat aagaatcaat agttattata
    16921 aagacatatt aaaaaattca cagtctgatg aaagtgcaaa agaatttata aaagaaagac
    16981 taaattcagc aacaggactt atgaaaaata tagagagtag gaaaactaca gtacttaaga
    17041 ttgcagaaga gatagtaaaa gctcaagatg aatttttgag aaaagggaca aaatatataa
    17101 aacctttgaa aatgaaagac atagcagaaa aattagaatt tcatgaatct actataagta
    17161 gaggtgtaaa tggtaaatat atgctaacac cttttggcgt atatgaattt aggtactttt
    17221 tcagtagcgc cattgagact gagaataatg aaatggcatc tagtacaagc ataaaaaaaa
    17281 ttataaagga aacaataaaa gacgaaaata aaaagaaacc tttaagtgat gaccatatat
    17341 ctaagttatt aaaagaaaaa ggaatcaatg ttgcaagaag gacagttgcc aaatatcgtg
    17401 aagaattagg aatcccatct tcaagtaaga gaaaagaata ctaataagta aaagacaaat
    17461 atcagtatgg taaggggaaa aggctaatta agagatgaat gtagattggg ctaaaaattt
    17521 tataaagtaa aataatagaa tatggaaaag tattaaaatg ctataaggta ttaaaaaact
    17581 ggggaacatt aaaacattaa gatagcaaag aacataaaaa actaaagaag attaaaacat
    17641 taaagaacat taggctttga ggaatattag aactttaaag aatattaaaa tttataaaat
    17701 ataaaaatac taaaaagaat tgaaaaactg gggaacatta aaatattgta
//
LOCUS       DAMJWP010000059        17253 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.060, whole genome shotgun sequence.
ACCESSION   DAMJWP010000059 DAMJWP010000000
VERSION     DAMJWP010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17253
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.060"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            28..774
                     /locus_tag="PC700_001690"
     CDS             28..774
                     /locus_tag="PC700_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HDF2581002.1"
                     /translation="MKIENLSIDDIKRGYIFNDDLNCYSCIKCTKTYEVGEIYKIGNR
                     FFEASKAIELHMELEHSDYLEQLLNSDSKYNTLTENQKQLFQLFISDLPDKEIAKQVG
                     VSTSTIRHQKFIFREKAKQAKLYLALYESVFEDKSNKSSTIIPIHEHARMVDERYVVT
                     EEERKHILDTSFESMNPLKLKVFSSKEKKKVVILSKILEQFERGKKYSEKEVNQILES
                     IYDDFATIRRYLIEYGFMSRNKECTKYWLT"
     gene            836..1510
                     /locus_tag="PC700_001691"
     CDS             836..1510
                     /locus_tag="PC700_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2581003.1"
                     /translation="MNDELKSILYNKGVDIVRFVDISEFPINQTQGFSKAILFCIGLS
                     KKFITDIYNNLPTDSDEFLEKEEKVEELADWISKYIQNKGYRAYSQSEKNNLEHGYFE
                     KAYINPEMQSGISPLPHKTIANISGIGFMGKNNLFVTEEYGCAFSMCTVLTDAPISVE
                     RYPLIDSKCLNCNVCVENCPAKAIHGNEWTLPGKRESIIDVSKCFCALRCMMSCPWSL
                     RYANQK"
     gene            1878..3224
                     /locus_tag="PC700_001692"
     CDS             1878..3224
                     /locus_tag="PC700_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HDF2581004.1"
                     /translation="MEIETKTPDWINVLELNSKIDITWKYDVSNMIQDIVSDKSLEGS
                     IIYFPKGNYLFEKGIKISQQITLQGDSYYGGDNQVSNLDKKQSIVGTTNFITRGVSNI
                     SIITLEGTSQCIKNINFYYDSHDIEKIPPKNVSAITEYGETQGLFHFEHLFISGFSGI
                     GIEIPYYSTGNDIIVSSCGLGMRLGEKSMLSSSKIYECKNGMEITTGVSLNNVRIEEI
                     REIGINNKGFGFNLIMNLMVDQCGYCGFMFEKMCYSQITARITKCGQYYKSVDYNTYE
                     NMEDRKEEAYSIFYGDILENCNIVLMNSNIDTLENDSLDEHKIYVIKANETNKVMLTC
                     YAEADELIQCAKGNLLLENGENTYKFYNGEICSVGGVQISDIDEKDLIKIIENTLYID
                     DKGKYLMVPRLKKNTVIYSVLDSIEEINKLHGGIWEQIDVKVLAGESMYYYKKIKD"
     gene            3251..4651
                     /locus_tag="PC700_001693"
     CDS             3251..4651
                     /locus_tag="PC700_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HDF2581005.1"
                     /translation="MEESIIIKDISGWINVLDYGICNDETKDVSSKLQEIIDAAPEDS
                     IIYFPKGKYLFESGVKIKKRLTLCGDSYILANVPKNTAGVTQFTFKGKKNPKSENIAI
                     ITAKSVRHCIKSICFYSDSCDRDMTSNHEPPTDGKPRFHHNVIINYRNISAIVCEDKQ
                     GEPGHYENLFFSGFSGIALDMPNNSTANDITVFTSGLGISTGENSTIANSKVWGCVDG
                     MIISTGTFINGMRVEEIKKVGIKNIGRGLNFITNITIDQCGYCGVWFDSISNVQISGN
                     ITRCGQYYYDVSYDNYLMLDNRVKEAYSLFYGNLLESSNIVLSNTNKDNWEDFKEDKH
                     KVYLVEALETKNVLLTCNIDATDWIVKSEKGNLTYNNMRQTVIFYDGKLSSINGIGIS
                     DEDYGDKVKIKKGNIYINSKDKVIKMHKPEVGDIMSTIIKDENILSAHYGGTWGKIGE
                     KIEFGITVHYYKLIEK"
     gene            4861..6297
                     /locus_tag="PC700_001694"
     CDS             4861..6297
                     /locus_tag="PC700_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HDF2581006.1"
                     /translation="MEGRTGIKDGRWINVVERGIKNDGSYNIGPDLQDIIDEIETDFC
                     VLYFPKGKYLFTSGVKINKEIVLQGDSNSPTETTQFITRGVENMSIITLTGKKQCIKN
                     INFYSDSCEIQVNEEPPTKGNPKYHYEMIINKNEKNVEMNNVSAILYDNSKNMIKGLG
                     HYENIYISGFSGTGIRIPYYSIVNDITVSSCGLGIDTGIDTIISNSRISKCQNGMRIR
                     TGTSINNVRIEKIQKVGLISSQVKDYEGYGSYKINNITIDQCGYCGFLFDSVKGSYFS
                     GIIRRCGQYYYNTDYDTYLSIKDRVEEAYSLFYGNYFTECNIDLVSDNKDNWDDGLEY
                     KHKVYVFKTLQIPKLTLRCNIDAYDYIVKSVHGGGNLMLKNNFNNYIFIDSSSPEVIN
                     GINFNYQKNGNLLRLNNGKLSYKNGKEETAIFRPNYGLIVASTDSECEQIETSYGGKW
                     EKLGSEVIGKETVYYYKHHEPDKTNQIK"
     gene            6641..7585
                     /locus_tag="PC700_001695"
     CDS             6641..7585
                     /locus_tag="PC700_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HDF2581007.1"
                     /translation="MIVSVSRRTDIPAFYSKWFFNRLKEGFVYVINPMNPKQVSKIEL
                     NPHTVDCFVFWTKDVTPMLYDLNKLKDYKYYFHYTITSYGKEVETCILDKRKVIDSFK
                     ELSRKIGKERVILRYDPIFLSEKYTVDYHIKAFASLCNQLDGFTEKCIISFIDLYKKT
                     KFNTKALHIKPIEITEIETLSKEISKIAHKHGIILEMCSEEYNLSKFGIGKSKCIDDR
                     LISKIIGCEVEVKKDSTQRDICGCVKSVDIGQYNTCRHYCLYCYANFNYAKVEENCRL
                     YKEDNKLLIDTLRDDAKISIRDMKTIKVDKNKQISIFD"
     gene            7754..8686
                     /locus_tag="PC700_001696"
     CDS             7754..8686
                     /locus_tag="PC700_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HDF2581008.1"
                     /translation="MNLLKKYPIPIASLILSIFTLGNLLQSYSESLRNVIGFIGFILY
                     AIYIVKIILLRKGVKEQLENPLISSTFPTITMATMIFATYIKPLSLELSIMIWSIGIV
                     GHLVLIVLFSKKFLVKFSIKTVFPSWYIVYVGIVAASVTAPAFNQLLIGKIAFWIGFV
                     SYIVLIPVILKRVWIIKEMPEPSLPSIAILAAPGSLLLSGYINSFSDKNNVVLYLLFI
                     LSIVFYIIVLGYLPKLLRLPFYPSFAAFTFPMAISAMGMKLMNGYLTKSGHFISWISY
                     ASKLEEIIATVIILYVLVLFIIYLSKSETTSKAK"
     gene            8791..9258
                     /locus_tag="PC700_001697"
     CDS             8791..9258
                     /locus_tag="PC700_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C_GCAxxG_C_C family protein"
                     /protein_id="HDF2581009.1"
                     /translation="MKTKEEYLKEVRNISEEYFRKGEFFCSEAVLQTINDALGQPLSP
                     EITKLASGFPIGLGKAQCLCGAISGGEMALGIVYGRVHGETMNPKMFEHAKGLHDYIK
                     KEYGATCCRAITKKWDGDNFMSPERKEHCIKITGQVTEWVADKLIEDEKLNIK"
     gene            complement(9502..10356)
                     /locus_tag="PC700_001698"
     CDS             complement(9502..10356)
                     /locus_tag="PC700_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009912760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HDF2581010.1"
                     /translation="MLTIIIALLPVMGWGLMPIVANLKKSSPHEQLLGTSISAFIFST
                     IITLVIHPEITFFSFCISMISGIFWSLGQLMQFKAIQIASVSKVMPISNGSQLTFTTL
                     LAVILFNEWTSSKMLLIGSLSILCIILGILLTNYQTKKSTDKNMLKCVGVVLLSSLFL
                     SLYVVTNQIFLIEGYRIILPQSVGMLITSSIIVKYFSKEIVYRENVLFNLITGILWSI
                     ANLGMFITTNTLGVTISFSISQSCVIVATLGGIIFFKEKKNKKEWLAIFIGIILIMSG
                     VFMLSIIK"
     gene            10522..12486
                     /locus_tag="PC700_001699"
     CDS             10522..12486
                     /locus_tag="PC700_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2581011.1"
                     /translation="MLTDMIQNREIKIIKFLLKEGHTTVKAIADDLDVSEKTVSKSLK
                     EVEAVLKKIGLSLIRKPKIGVYIDGDLKKILPYLDNKGSQIPTTREERVIYIYSVLLK
                     SNDYITIQQLSDELYISRGTIERDLSEVEKLLKNEGISIYKKPSKGIKLNISERDKRQ
                     LTAKFIHKFWSRNWYIKQEQESFYQAFEKIQADVNGIFSEDDLSIIIDILRNFSRERK
                     FEFSDYEFQSLVIHLAIAVERIRKGEYIEESLYIGNIENNQMSNTNYLAKQLEEKFSI
                     LLPKSEIGYINLHLVAADQHRDTNELKNTSTNPCETSTNNISNLREIIKNTLYETAYD
                     KDLIDGLTSHVHSSINRLKYGLSIKNPYVNTIKQNFSLSFEQALDLKKVIEFIYDIRI
                     NDDECAYIALHFEAFRERMKELPQNIRVILVCSTGLGSSRLLSARIKKYFPMIKIEKV
                     LSVQELVSSKIDADLVISTIYLELETIPTIVVSPILNESDIRTLERNIQNVSCCTSKK
                     YKSFRNLIFEENILLNIEASNYEDAIKYITDNLIKKGFVKEGIAKSAIERENLSYTSF
                     QSIATPHANPSYIKKSNISVATLKNPITWGNEKVEVIFFISLENDKNLELDEIYEVFY
                     DFIDNKKNINDILSATSTKEVYDLLLEESI"
     gene            12486..12947
                     /locus_tag="PC700_001700"
     CDS             12486..12947
                     /locus_tag="PC700_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2581012.1"
                     /translation="MKISDVLKKEQIVFNLDTNTKEETIKKLSSIFVENGIVDNEEEY
                     VKSVLEREEHSTTGIGNGIAIPHGKSDAVKKSSIIFAKTKHKIEWDALDEKPVDSIFL
                     LAISNIDKDSNHLVLLSKLATKLMDDDNVDALKLANSEQEIINIFSEEGEM"
     gene            12950..13270
                     /locus_tag="PC700_001701"
     CDS             12950..13270
                     /locus_tag="PC700_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDF2581013.1"
                     /translation="MKRKIIAVTACATGVAHTYMAAQALKKASKKMGHLIKVETQGAT
                     GIDNELTTKDAQIAEVVIFAVDTKVRNAERFEGKEILEVPVNAPIKDAEKVINDALKL
                     IDTK"
     gene            13286..14386
                     /locus_tag="PC700_001702"
     CDS             13286..14386
                     /locus_tag="PC700_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDF2581014.1"
                     /translation="MLKKIVSELKKHVLTGISYMIPLVIAGAMIMAISRVGGSFYNIP
                     DIWDAKYAESASSIVRLLHDLDGFGGTALGLMFPVIAAFIGFSIADKLAIVPGLVGGM
                     IAKDIGAGFLGALAVGLIAGYTCLFIKNHVKLPKSAASIVPIFIVPVFGTLITVVLIN
                     YVIGIPFATLNTGLENWLNGLSGTNQILMAAIIGAMMAVDLGGPVNKAALTTSLALLT
                     SGIYAPNTAAMVGIVIPPLGLGLATILAKQKYNKQLREAGKSSLIMGLIGVSEGAIPF
                     AVESPLKVIPSCVIGTAIASAMAVGLGSVNATPISGFYGWFTVENWPMYVLSIAVGTV
                     IVAGLVIVLRGNPVIEDDEFEDDDFDEAEWES"
     gene            14506..17106
                     /locus_tag="PC700_001703"
     CDS             14506..17106
                     /locus_tag="PC700_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HDF2581015.1"
                     /translation="MIKAHIVNHTHWDREWYFTSGDALVLSEQLFTDVIDELERNPDV
                     SFVLDGQLSILDDYVQLHKEKIEIIKKLIKEDRLHIGPWFTQTDAFFARGESILRNLM
                     IGIFESKKYGKYMKIGYLPDTFGFNAQMPILLKHVGLDNIFFWRGIHLGKHVQSPYFK
                     WKSLNGESYIYAVNMPHGYGTGMLLEPSLKYVNGRLDPAIDFIKSYTDVDEVLIPSGN
                     DQLNIIGDFKNKIKAINDIGKYSYITSSYQEFLKYIKSIESLESYRGEFREPVLARIH
                     KSIGSSRMDIKLACDKLENKLIKRIEPLLVIAKKSKIEISNQLLINTWKKLLEGQAHD
                     SLAGCVTDTVTDYILHRIREANEICDSIENTIVKKISEGLKLSKNDILVFNTEAKRFN
                     GYKEIQVVSDSKNIYFKDNLDATIIEETYVKPRENVLEETPAGNVFIEESGYYILKVR
                     INVQLPALGYKVVSFESSDKEMVSLDKSNDTFISNDNYKIIYEDGSLNLQLKDGTYIS
                     NFLILKDSGNAGDTYDFSPLKNDEDIKLSFDRVYTEKTLGYEKLVVKGTTLLPLTLED
                     RKNKYLNGKLNVKVSIVLSKENPLMDVKLCVDNTIYNHRLRVHIKTDIKDNKNIASLP
                     FGYITRENGVLDNWEDIYSEMPIDLEPLESNITLTNQNRSCTVFTRGIKEYQHIEDEI
                     ALTLLATTDELGKPDLLYRPGRASGDTTKKGHIRIKTDKAQMLKKLEFSFAIYMDSKS
                     FDELEIANLTHNYLRECVNYQLQDYNFFLYRIDNKIQKSIVNKTVSRELEIISLPEKY
                     LISACYPSYYSKDKFIIRVENPSSNRMILDEQIFKNRNGKIVNAIEDVEEEQVYEIAP
                     FDVISILLDL"
BASE COUNT         6856 a         1784 c         2986 g         5627 t
ORIGIN      
        1 tgtaaattgt gaaatggagg tagtaacatg aaaattgaaa atctttctat tgatgatatt
       61 aaacgaggct atatatttaa tgatgattta aattgttata gttgcattaa atgtacaaaa
      121 acatatgagg taggagaaat atataaaata ggtaatagat tctttgaagc ttcaaaagct
      181 attgaacttc atatggaatt agaacatagt gattatttag agcaattgct taatagtgat
      241 tcaaagtaca acacattgac tgaaaatcaa aaacaattat ttcaattatt tattagtgat
      301 ttaccagata aggagatagc aaaacaggtt ggagtatcca catctactat acgccatcaa
      361 aaatttattt tcagagaaaa agcaaaacag gctaagctct atttagcact atatgaatct
      421 gtatttgaag ataaatctaa taaaagtagt actataatac caatccatga acatgctcga
      481 atggtagatg aaagatatgt agtaactgaa gaagagagaa aacacatttt ggatacatca
      541 tttgaaagta tgaatccttt aaaattaaaa gtattttctt caaaagaaaa gaaaaaggtc
      601 gtaattttat ctaagatttt ggaacaattt gaacgtggga agaaatattc agaaaaggaa
      661 gttaatcaga ttttagaatc tatatatgat gattttgcaa caattagaag atatttaatt
      721 gagtatggct ttatgagtag aaataaagaa tgtacaaagt attggttaac ataattaaaa
      781 taataaattt tactaatgtg taatgttatt tttatctagg ggggaggagt taaatatgaa
      841 tgatgaatta aaatctattt tatacaataa aggtgtagac attgttcgct ttgttgatat
      901 atctgaattt ccaataaatc aaacacaagg tttttctaaa gcaattttat tttgtatagg
      961 gttatcaaaa aaatttatta cagatatata caataactta cctacagata gtgatgagtt
     1021 tttggaaaaa gaggagaaag tagaagaatt agctgactgg atttcaaaat atattcaaaa
     1081 taaaggatat agagcgtatt ctcaatcgga gaaaaacaat ctagaacatg gatattttga
     1141 aaaagcctat attaatccag aaatgcaatc agggattagt cctttaccac ataagacaat
     1201 agcaaatatt agtggcattg gatttatggg aaaaaataat ttgtttgtaa cagaggaata
     1261 cggatgtgct tttagcatgt gtacagtact tacagatgct ccaatttctg ttgaaagata
     1321 tcctttgatt gattcaaaat gtttaaattg taatgtttgt gtagagaact gtcctgcaaa
     1381 agctatacat ggaaatgaat ggactttacc tggaaaaaga gaatctatta ttgatgtttc
     1441 aaagtgtttt tgtgcattga ggtgtatgat gagttgccca tggtcattaa gatatgcaaa
     1501 tcaaaaataa ctttactaga gaaggtatta aattgaatat tataaaattt ttaaaatgta
     1561 agattatata agatttctca aaataaaaat ttgtatagaa cactcagaaa tgagtgtttt
     1621 tctattggga ttaaattata ttgatacaaa gcttaactat tttaatcatt atataaaatt
     1681 tgtattaaaa aattaagaat aacttgctaa tagtaaaaat ataatgagat tataattgat
     1741 ttataatata actgtaatga tgttgtaaaa tgaaaattgt catctcaaaa ttcccaaaaa
     1801 gagtaaaagt cacttcggtt atagtcaaaa aattttttct gtgaaaagaa tttagattta
     1861 aaagggagga attaatcatg gaaattgaaa ctaaaactcc agattggatt aatgtactag
     1921 aattaaacag taaaatagat attacttgga aatatgatgt aagtaatatg atacaagata
     1981 ttgtctctga taagtcacta gaaggttcca ttatatattt tccaaaaggt aattacttat
     2041 ttgagaaagg tattaaaatt agtcaacaaa taacacttca aggagattct tattatggag
     2101 gagataatca agtatcaaat ttagataaaa aacaatcaat cgtaggaaca accaatttta
     2161 taacccgagg agtttctaat ataagtatta ttacattgga aggaacaagt caatgtatca
     2221 aaaatattaa tttttattat gatagtcatg atatagagaa aataccacct aaaaatgtat
     2281 ctgctattac agaatatgga gaaacacaag gactatttca ctttgaacac ctgtttattt
     2341 ctggattttc aggaataggg attgaaattc catactattc aactggcaat gatatcattg
     2401 ttagttcatg tggtcttggt atgaggcttg gagagaagtc aatgctttct agtagcaaga
     2461 tttatgaatg taaaaatgga atggagatta ctactggtgt tagcttaaat aatgtgagaa
     2521 tagaagaaat aagagaaata ggtattaata ataaaggatt tggttttaac ctgattatga
     2581 atcttatggt cgaccaatgt ggatattgtg gatttatgtt tgaaaaaatg tgttatagcc
     2641 agataactgc cagaattact aaatgcggtc agtattataa aagtgttgat tataatactt
     2701 atgagaatat ggaagataga aaagaagaag cttattccat tttttatgga gatattttag
     2761 aaaattgcaa tattgtattg atgaatagta atattgatac cttagaaaat gattcacttg
     2821 atgaacataa gatatatgta atcaaggcaa atgaaacaaa caaagtaatg ttaacatgtt
     2881 atgctgaagc agatgagctt atacagtgcg caaagggcaa cttgttgcta gaaaatggtg
     2941 aaaatactta taaattttat aatggtgaga tttgttctgt aggaggtgtt caaatttctg
     3001 atatagatga aaaagattta atcaaaataa tcgagaacac tctgtatata gatgataaag
     3061 gtaagtactt aatggttcct agattaaaaa agaatactgt aatatattca gtactagatt
     3121 ctatagaaga aataaataaa ttacatggtg gaatatggga acagattgat gtaaaagtat
     3181 tagctgggga atctatgtac tattataaaa agataaaaga ttaaattatc aaactaggag
     3241 gtagtgatta atggaagagt ctataataat taaagatata agtggctgga ttaatgtact
     3301 tgattatggg atttgtaatg atgaaacaaa agatgtaagt tccaagttgc aagagattat
     3361 agatgctgca ccagaagatt ctattatata ttttccaaaa ggaaaatact tatttgaaag
     3421 tggtgttaag attaagaaac gattaacctt atgtggagat tcttatattt tggcgaatgt
     3481 tcctaaaaac actgctggag taacacaatt tacttttaaa ggaaagaaaa atcctaaaag
     3541 tgaaaatata gcaattataa cagcaaaaag tgtaagacac tgtattaaga gcatttgctt
     3601 ttattcagat agttgtgata gagatatgac atctaatcat gaacctccaa cagatggaaa
     3661 acctagattt catcataatg taattataaa ttatagaaat atatcggcta ttgtatgtga
     3721 agataaacag ggagaaccag gtcattatga aaacttgttt tttagtggtt tttctggaat
     3781 tgcacttgat atgcctaata attctacagc aaatgatatt acagttttta catccggact
     3841 tggaattagt acaggggaaa attcaacgat tgctaacagt aaggtttggg gctgtgttga
     3901 tggaatgata atttctactg gaacttttat aaatggtatg agggtagaag aaataaaaaa
     3961 agttggaatt aaaaatattg gtagaggttt aaattttatt acaaatatca caattgacca
     4021 atgtggatat tgtggagttt ggtttgattc tatatccaat gttcagatat caggaaatat
     4081 cacaaggtgc ggacagtact attatgatgt aagctatgat aattatttaa tgttggataa
     4141 tagagttaaa gaagcatatt ctttattcta tggtaattta ttggagtcaa gtaatatagt
     4201 tttgtcaaat accaataaag ataattggga agatttcaaa gaggataagc ataaggtgta
     4261 tcttgtagaa gcacttgaaa ccaagaatgt actattaaca tgcaatatag atgctactga
     4321 ctggattgtt aagtcagaaa aaggaaattt aacgtacaat aatatgcgac aaactgttat
     4381 attttacgat ggaaaactaa gtagtatcaa tggaattggt atatctgatg aagattatgg
     4441 ggataaagtc aagataaaaa aaggaaatat atatataaat agtaaggata aggttattaa
     4501 gatgcataaa ccagaggtag gtgacattat gtcaacaatt attaaagatg aaaatatact
     4561 ttccgctcat tatgggggaa catgggggaa aattggtgaa aaaatagagt ttggaataac
     4621 tgtacactat tataaattga tagagaaata agtaaatacg tagtatttga atagatgtat
     4681 tttattggag agctaggact atagttttaa tgtaaatggt ttggtaatag catggattga
     4741 ataatataaa aataatataa aaaattaatt tgattatgta aaattctatg aagtagtaaa
     4801 cctgtgactt tagtcatggt aggttcaaat taagtaaatg ggaaaggaat atataatatt
     4861 atggaaggaa gaactgggat taaagatgga agatggatta atgtagtaga aagaggtatt
     4921 aaaaatgatg gttcgtacaa cataggtcct gatttacagg atattattga tgaaatagaa
     4981 acagatttct gtgtcttata ttttccaaaa ggaaaatact tatttacaag tggtgttaaa
     5041 attaataaag aaattgtatt acaaggtgat tctaattcac ctacagagac tacacaattt
     5101 ataactcgtg gagtagaaaa tatgagtatt attacattga cagggaagaa acaatgtatt
     5161 aaaaatataa atttctattc tgatagttgt gagatacagg taaatgaaga accaccgaca
     5221 aaaggtaatc ctaaatatca ttatgagatg attattaata agaatgaaaa aaatgttgag
     5281 atgaataatg tatcagcaat tctgtatgat aatagtaaga atatgattaa aggtctaggt
     5341 cattatgaaa atatttatat aagtggtttt tcgggtacag gaattaggat accatattac
     5401 tcaattgtaa atgatattac agtttcatct tgtgggttgg gtattgatac aggaatagac
     5461 acaattattt ctaatagtag aatatctaag tgtcaaaatg gaatgaggat tagaactgga
     5521 actagcataa ataatgtaag aattgaaaaa atacagaaag ttggtcttat aagtagtcaa
     5581 gttaaggatt atgagggata tggaagttat aaaattaata atattacgat tgaccaatgt
     5641 gggtattgtg gttttttatt tgactcagtg aaaggtagtt atttttctgg tattataaga
     5701 agatgtgggc agtattatta taatacagat tatgacacat atttaagtat taaagataga
     5761 gtagaagaag catattcttt attttatgga aattatttta cagaatgtaa tattgattta
     5821 gttagtgata ataaggataa ctgggatgat ggcttagaat ataaacataa agtttatgta
     5881 tttaaaaccc ttcaaatacc aaaattaaca ttgagatgca atatagatgc ctatgattat
     5941 atcgttaaat cggtacatgg aggaggtaat ttaatgttaa aaaataactt taacaattat
     6001 atatttattg attcatctag tccagaagtt attaatggaa ttaattttaa ttatcaaaag
     6061 aatggaaacc tgttaaggtt aaataatggt aaattaagtt ataaaaatgg aaaggaagaa
     6121 acagctatat tcagacctaa ttatggtctt attgtagcat caacagattc agaatgtgaa
     6181 caaatagaga ctagctatgg aggaaaatgg gagaagcttg gttctgaagt aattggaaaa
     6241 gaaacggtat attattataa acatcatgaa ccagataaaa caaaccaaat aaaataaaat
     6301 ttgtattata taatgatata aagttttaat atatagtgtt tacctttaat gtaatattgg
     6361 tatatttgta tagtattaaa tttgttaaaa agtaaaattg tagggtgctt gaaaatagca
     6421 ccttttttag tttcattata aaattagttt tgaaagttta ggaataatgt atatttattt
     6481 ttaaagaatt ataaccttat taaggatatt ttaaatgcta gaaatgaatc agtaaatcat
     6541 actggaatta tcttaaatgt tttgattaaa ttaagtattt agagctataa ttatattaaa
     6601 aatatattag gacataatta tattactatg gaggttaaat atgatagtaa gtgtgagtag
     6661 aagaactgat atacctgctt tctatagtaa gtggtttttt aacagactta aagaaggttt
     6721 tgtttatgta attaatccaa tgaatccaaa acaggttagt aagatagagt taaatccaca
     6781 tactgttgat tgttttgtat tttggacaaa ggatgtaact ccaatgttat atgatttaaa
     6841 taaattaaaa gactataaat attattttca ctatactata acatcatatg gaaaagaagt
     6901 agagacatgt attttagata aaagaaaggt aatagattca tttaaggaat tgtctagaaa
     6961 aataggcaaa gaaagagtaa ttttaagata cgacccgatt ttcttaagtg aaaagtacac
     7021 agtagattat catatcaaag catttgcgag tttatgtaat cagcttgatg gatttacaga
     7081 aaaatgtata ataagtttta tcgatttata taagaaaaca aaatttaata ctaaagcatt
     7141 acatataaag ccaatagaaa ttacagaaat agaaacatta tcaaaagaaa tttctaagat
     7201 tgctcataag catgggataa tacttgaaat gtgttctgaa gaatataatt taagtaaatt
     7261 tgggattgga aaaagtaagt gtattgatga tagattaata tcaaaaatta taggatgtga
     7321 agtagaggta aaaaaagata gtacacaaag agatatttgt ggatgtgtta aaagtgtcga
     7381 tataggacaa tacaatactt gcagacatta ttgtctgtat tgttatgcta actttaatta
     7441 tgcaaaagta gaagaaaact gtaggcttta taaagaagat aataaattac tcatagatac
     7501 tttaagagat gatgcaaaaa taagcattag agatatgaag actattaaag tagataagaa
     7561 taaacaaatt agtatatttg attaaaataa atatactaat taccttaata tatcaagaat
     7621 atagatactt ttgataacaa aattaaactt aatagatatt ttctatataa gaaagatggt
     7681 cataatatgt caatttgacc atctttttga tataattaaa acacaaataa tttagtaaca
     7741 aaggaggcag aacttgaatt tattaaaaaa atatcctata cctattgcaa gtttgatttt
     7801 gtcaattttt actttaggta acttattaca atcttatagt gaatcattac gtaatgtaat
     7861 tggatttata ggatttattt tgtatgcaat ttatatagtc aaaataatat tacttagaaa
     7921 gggtgttaaa gagcaattag agaacccttt aatatcaagt acatttccaa ctataacaat
     7981 ggcaacaatg atatttgcta catatataaa accattatct ttagaattaa gtattatgat
     8041 ttggagcata ggaattgtgg gtcatcttgt gttaatagtt ttatttagta agaagttctt
     8101 agttaaattc tcaataaaaa cggtatttcc atcttggtat atagtatatg ttgggatagt
     8161 tgcagctagt gttacggcac cagcatttaa tcaacttttg attggcaaaa ttgctttttg
     8221 gataggattt gtatcttata tagttcttat accagtaatt ttaaagagag tatggataat
     8281 caaggaaatg cctgaaccat ctttaccaag tatagctata ttagctgcac cagggtcact
     8341 attattgtct ggatatatta attctttttc agataaaaat aatgtagtat tatacttgtt
     8401 attcatatta tcaattgtat tttatattat agttttggga tacttaccga aattattaag
     8461 attgcctttt tatccgagtt ttgctgcatt tacattccct atggcaataa gtgcaatggg
     8521 tatgaaacta atgaatggtt atttaactaa atcaggtcat tttataagtt ggatttcata
     8581 tgcttctaag ttagaagaga taattgctac agtgattatt ttatatgttt tagttttatt
     8641 tattatatat cttagtaaat ctgaaactac atctaaagct aaataagaat tattaaatat
     8701 gtgtatttaa taggaatatt gatataaata tatgaagata agataaacca tagaaactta
     8761 atttagctta attttattgg aggaaaacaa atgaaaacaa aagaagaata tttaaaagaa
     8821 gttagaaaca tatcagaaga atactttaga aaaggagagt ttttttgctc tgaagcagtt
     8881 cttcagacaa taaatgatgc tttaggccaa cctttaagtc cagagataac taaattagca
     8941 tcaggatttc cgataggact tggaaaagct caatgccttt gtggagcgat atctggagga
     9001 gagatggctt taggtatagt atatggaaga gtacatggag aaactatgaa tccaaagatg
     9061 tttgagcatg ctaaaggttt gcatgattat attaaaaaag agtatggtgc aacatgttgt
     9121 agagctatta caaagaaatg ggatggtgat aactttatgt ctcccgaaag aaaagagcac
     9181 tgtattaaaa taacaggtca agttacagag tgggttgcag ataagcttat agaagatgaa
     9241 aaacttaata tcaaatagtg taatattagt tggtgaattt ataaagttaa atttacttat
     9301 agtataagat acttgatatt ctcgtattga tagaaaaatt tatagcatat gtatttaatg
     9361 aaatatactt atatttagag tgtggtttaa tttcataaat acatatgcta ttattttaag
     9421 tatataagca aatttattta aataaataga aacagttaat ttatatgtac aaaactatat
     9481 atgattagtg taaataaata cctattttat aatactcaac ataaagacac cagacatgat
     9541 tagtattata ccaataaaaa tagcaagcca ttcttttttg ttcttctttt ctttaaaaaa
     9601 tataatccct cctaaagtgg ctactatcac acaactctga gagatagaaa aacttattgt
     9661 aactcccaat gtattagtcg ttatgaacat acctaaattt gctattgacc acaatatacc
     9721 agtaattaaa ttgaataaaa cattttccct atatacaatc tctttagaaa aatacttgac
     9781 aataatagat gaagttatca acataccaac agattgaggt aatataattc tatatccttc
     9841 aattaaaaat atttgattag ttacaacata taatgataaa aatagagatg ataataatac
     9901 aactccaaca cattttaaca tatttttatc tgtagatttt ttagtttgat aattagtgag
     9961 aagtatacca agtattatac ataatattga taaagaacca atcagtaaca ttttagaact
    10021 ggtccattca ttaaacaaga ttacagctaa taatgtcgta aaagttaatt gtgaaccatt
    10081 tgaaattggc attactttag atacactagc aatttgaatt gctttaaact gcattaattg
    10141 tcccaaactc cagaatatgc cagaaatcat acttatacaa aatgaaaaaa atgtaatttc
    10201 aggatgtata accagagtta ttatagtgga aaatataaac gcacttatgg atgtacctaa
    10261 aagctgttca tgaggagaac ttttctttag atttgcaact atgggcatca atccccatcc
    10321 cattacagga agtaaagcaa taattattgt cagcattaaa ctatttcctt tcatattaaa
    10381 tttagacagc ttttaattta tcatctaaaa ttaaagtgtt caatacataa aaattaccgt
    10441 atttaatcag gaaatattag tagtttttta gaaaaacatt ttccattaaa ataaagataa
    10501 agggaaggag taaaattgaa aatgttaact gatatgattc aaaatcgtga aattaaaata
    10561 ataaagtttt tattaaaaga aggtcataca actgtaaaag caatagcaga tgatttagat
    10621 gtttcagaaa aaacagtttc aaaatctctt aaagaagtag aagcagtgct taaaaagata
    10681 ggtctttctt taataagaaa acctaagata ggtgtttata ttgatggaga tttaaaaaaa
    10741 atacttcctt acctagataa taaaggaagt caaattccta caaccagaga ggagagagtt
    10801 atctatatat attcagtact tttaaagagt aatgattata ttactataca acaactttct
    10861 gatgaactat atataagtcg tggaactatt gaaagggatt tatcagaagt tgaaaaactg
    10921 ctaaaaaacg aagggatttc catatataag aaacctagta agggaataaa gttaaatata
    10981 agtgaaagag ataaaagaca acttactgct aagtttatac ataaattttg gagtagaaac
    11041 tggtatatta agcaagagca agaaagcttt tatcaagcat ttgaaaagat acaggcagat
    11101 gtaaatggta ttttttcgga agatgattta tcaataatta ttgatattct taggaatttc
    11161 agcagagaaa gaaaatttga gttctctgat tatgaatttc agtcattagt tattcatttg
    11221 gcaatagctg tagagagaat tagaaaaggc gaatatatag aagaaagttt gtatattggt
    11281 aatatagaaa ataatcaaat gtcaaatacc aattatttag caaaacaatt agaagaaaaa
    11341 ttttcaatat tattaccaaa atctgaaatt ggatatataa atttacattt agtagcggct
    11401 gaccaacata gagatactaa tgaactaaaa aatacttcaa ctaatccttg tgaaacaagt
    11461 acaaataata tatcaaatct tcgggaaatt ataaaaaata cattgtatga gacagcttat
    11521 gacaaggatt taattgatgg attgacttca catgtacatt catcaataaa tagactgaaa
    11581 tatggactta gtattaaaaa cccatatgta aatacaatca aacaaaactt ttctttatca
    11641 tttgagcaag ctttagattt aaagaaagta attgaattta tatatgatat tagaataaat
    11701 gatgatgaat gtgcttatat agcattacat tttgaagctt ttagagaaag aatgaaagaa
    11761 cttccacaaa atattagagt aattctagta tgcagtacag gattaggttc atctaggtta
    11821 ttgtctgcaa gaataaaaaa atactttcca atgataaaga ttgaaaaagt actttccgta
    11881 caagaattag tatccagtaa aattgatgca gatttagtaa taagtactat ttatttagag
    11941 ttagaaacaa ttccaaccat agtagttagc cctattttaa atgaaagtga tataagaacc
    12001 ttggaaagaa atatacaaaa tgtttcatgt tgtacttcta agaagtataa atctttcaga
    12061 aacctaatct ttgaagaaaa tatattgtta aatattgaag cttcaaacta tgaagatgca
    12121 attaagtata taacagataa cctaataaaa aaaggttttg taaaagaagg tatagctaaa
    12181 agtgcaattg agagagaaaa tttatcttac acgtcttttc agtcaatagc tacacctcat
    12241 gctaatccta gttatattaa aaaatctaat atatctgttg caacgttaaa aaatcctatt
    12301 acatggggaa atgaaaaggt ggaagttatt ttctttattt cattagaaaa tgacaaaaac
    12361 ctagagcttg atgaaatata tgaagttttt tatgacttta ttgacaataa aaaaaatata
    12421 aatgacatat taagtgcaac ttccacaaag gaagtgtacg atttgctatt ggaggagagt
    12481 atatagtgaa aatatcagat gtattgaaaa aagagcaaat tgtttttaat cttgatacaa
    12541 atacaaagga agaaacaata aaaaaattaa gtagtatatt tgttgaaaat gggattgtgg
    12601 acaatgaaga agaatatgtt aagtctgttt tggaaagaga agaacattca actacaggta
    12661 ttggtaatgg tattgctatt cctcatggaa aaagtgatgc tgtcaaaaaa tcttctataa
    12721 tatttgcaaa gactaagcat aagattgagt gggatgcttt agatgaaaaa ccagtagata
    12781 gtattttctt attggctata agcaatattg ataaggatag caatcatctt gtactattat
    12841 ctaaattagc aactaaactt atggatgatg acaatgtaga tgcactaaaa ttagccaatt
    12901 cagaacaaga aataatcaat atatttagtg aagaaggaga gatgtagaaa tgaaaagaaa
    12961 aattatagca gttacagcat gtgcaacagg agttgcacat acatatatgg cagctcaagc
    13021 gttaaagaaa gcttcaaaaa agatgggtca ccttataaaa gttgaaacac aaggagctac
    13081 aggaatcgat aatgagttaa ctacaaagga tgcacaaatt gcagaagtag taatatttgc
    13141 agtagatact aaagtcagaa atgctgaacg ttttgaggga aaagaaatac tagaagtacc
    13201 agttaatgca cctataaagg atgcagagaa agtaataaat gatgcattaa agttaattga
    13261 cactaaataa atggagggaa atgtaatgtt gaaaaagata gtctctgagt taaaaaagca
    13321 tgtcttaact ggaatctcat atatgattcc attagtaatt gcaggtgcta tgataatggc
    13381 aatatctcgt gtaggtggtt ctttctataa tataccagat atatgggatg ctaaatacgc
    13441 agaaagtgct agtagtattg ttagattatt acatgatcta gatggatttg gaggtactgc
    13501 attaggatta atgtttcctg taattgctgc atttatagga ttttctatag ctgataaatt
    13561 agctattgta ccaggtcttg ttggaggtat gattgctaaa gatataggtg caggattttt
    13621 aggagctttg gcagttggtc ttatagcagg atatacatgt ttgtttatta aaaatcatgt
    13681 taaattacca aaatcagcag cttcaatagt accaatattt atagtaccag tatttggaac
    13741 attaatcact gttgtattaa tcaattatgt gataggtata ccttttgcca cattaaatac
    13801 tggattagaa aattggttaa atggattatc tggtacaaat cagatattaa tggcagctat
    13861 tataggggct atgatggcag ttgacttagg tggaccagtt aataaagcag ctttaacaac
    13921 atcacttgca ttattaacaa gtggaattta tgcacctaat actgctgcaa tggtaggaat
    13981 agttatacca ccactaggat tgggacttgc tactatacta gcaaaacaaa aatataataa
    14041 acagttacgt gaagctggga aatcatctct tataatgggt ttaattggag taagtgaagg
    14101 agcaattcct tttgcggtag aatctccatt aaaagtaata ccttcatgtg taattggaac
    14161 agcaattgct tcagctatgg cagttggttt aggttcagta aatgcaactc caattagtgg
    14221 attttatgga tggtttacag tagagaattg gccaatgtat gtgttatcaa tagcagttgg
    14281 aacagtaatt gtagctggat tagttatagt attgcgtgga aatccagtga ttgaggatga
    14341 tgaatttgaa gatgatgatt ttgatgaagc agagtgggaa tcttaaaatt taataactat
    14401 ttaagacgtg ttataagcta tattcaatat gaatcttata ctaaataaag tgttaaatat
    14461 tcagtttaga cttaaatata aaagataaag aacagaggag aataaatgat aaaagcacat
    14521 attgttaatc atacacactg ggatagagaa tggtatttta cttctggaga tgcattagta
    14581 cttagtgaac aactatttac agatgtaatt gatgaattag aacgtaatcc agatgtaagt
    14641 tttgtacttg atggacaact ttctattttg gatgattatg tacaattaca caaagaaaaa
    14701 atagaaatca ttaaaaagtt aataaaagaa gatagactac atattggacc atggtttact
    14761 caaactgatg cattctttgc tagaggagag tctattttaa gaaacttaat gattggaatt
    14821 tttgaaagta aaaagtatgg taagtatatg aaaattgggt atttgccaga tacatttgga
    14881 tttaatgcac agatgcctat actattaaag catgtaggtc ttgataatat attcttttgg
    14941 agaggtatac atcttggtaa acatgttcaa tcaccatatt ttaagtggaa aagcttaaat
    15001 ggagaaagtt atatatatgc agtaaatatg ccacatggat atggaactgg tatgctttta
    15061 gagccttcat taaagtatgt taatggacgc ttagacccag ctattgattt tattaaatca
    15121 tatactgatg tagatgaggt gttaatacct tctgggaatg accagttaaa tataataggt
    15181 gatttcaaaa ataaaattaa agcgattaat gatatcggaa agtattcata tataacgagt
    15241 tcatatcaag agtttttaaa atatataaag tccatagaat cactagaaag ctatagaggt
    15301 gaatttagag aacctgtatt agcaagaata cataaatcaa ttggttctag tagaatggat
    15361 ataaaattag catgtgataa actagaaaac aagctgatta aaagaataga gccattatta
    15421 gtaattgcta aaaagagtaa aattgaaata agcaatcagt tacttataaa tacatggaaa
    15481 aaacttttgg aaggacaagc acacgatagt ctagctggat gtgtaactga tactgtaaca
    15541 gattatattc ttcaccgtat aagagaagca aatgaaatat gtgacagtat tgaaaatact
    15601 attgtaaaaa agatttcaga agggcttaaa ttatctaaaa atgatatttt agtatttaat
    15661 acagaagcta agcgttttaa tggatataaa gagatacaag ttgtaagtga tagtaaaaat
    15721 atttatttta aagataattt agatgctact ataattgaag aaacttatgt aaaaccgaga
    15781 gaaaatgtat tagaagaaac acctgctggc aacgtattta ttgaagaatc aggatattat
    15841 atattaaaag taagaataaa tgtacagctt cctgcacttg gttataaagt tgttagtttt
    15901 gaatcaagtg ataaagaaat ggtatcactt gataaatcta atgacacttt tatatctaat
    15961 gataattata agataatcta tgaagatgga agcttaaatt tacaacttaa agatggaact
    16021 tacatatcaa actttttaat attaaaagat agtggtaatg caggcgatac ctatgacttt
    16081 tctccattaa aaaatgacga ggatataaaa ctttcatttg atagagtgta tacagaaaaa
    16141 actttaggat atgaaaagtt ggtggttaaa ggtactactt tactaccact tacattagaa
    16201 gatagaaaaa acaaatatct taatgggaaa ttaaatgtaa aagtaagtat tgtcttatcc
    16261 aaagaaaatc cattgatgga tgtaaaactt tgtgttgata acacgatata taatcataga
    16321 ctacgtgttc atataaaaac tgatataaaa gataataaaa acatagcatc attgccattt
    16381 ggatatataa caagagaaaa tggagtttta gacaactggg aagatattta cagtgaaatg
    16441 cctatagatt tagaaccttt ggaaagtaat attactctaa caaaccaaaa tagaagctgt
    16501 actgtattta ctagaggaat aaaagagtat caacatattg aagatgaaat tgcattgacc
    16561 ttacttgcta ctacagatga actaggaaag cctgatttat tatatagacc aggacgtgct
    16621 tctggtgata caactaaaaa aggtcatata agaattaaaa cagataaagc tcaaatgctt
    16681 aagaaattag aattttcatt tgcaatatat atggattcta aatcatttga tgaattggaa
    16741 attgcgaatt taacccacaa ttatctaagg gaatgtgtaa attatcaatt acaggattac
    16801 aatttctttt tatatcgtat agataataag attcaaaaat caattgtcaa taaaactgta
    16861 agtagagaat tagaaataat atctttacca gaaaaatatc ttatatcagc atgttatcct
    16921 tcttattaca gtaaagataa atttataata agagtggaaa atccaagtag taatagaatg
    16981 atattggatg agcaaatatt taaaaataga aatgggaaaa ttgtaaatgc aatagaagat
    17041 gtagaagaag aacaggtata tgaaatagca ccttttgacg ttatttcaat attattggat
    17101 ttataagtgt aaaagtaagt ttattgttta aagtctgaca gatattaatc tgccagactt
    17161 tttaatttgt ataaatttgg gtaaatatta atgaatagtt ttagtattta aatatgaatt
    17221 tagtatttaa tatagaattt attgaagtag aat
//
LOCUS       DAMJWP010000060        17184 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.062, whole genome shotgun sequence.
ACCESSION   DAMJWP010000060 DAMJWP010000000
VERSION     DAMJWP010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17184
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.062"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(<1..245)
                     /locus_tag="PC700_001704"
     CDS             complement(<1..245)
                     /locus_tag="PC700_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581016.1"
                     /translation="MLVRVKIVIGATIRYLIKHKVNYCNKKSLDKLELKYGNIDREKA
                     VKLEIFYQYLIELEYIEMSLLIRRLDTAITSLALVSI"
     gene            452..1270
                     /locus_tag="PC700_001705"
     CDS             452..1270
                     /locus_tag="PC700_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2581017.1"
                     /translation="MEKQYFTTGEFAKLCGISKQTLIFYDKIGIFSPEYKDKNNYRYY
                     SVYQYDTLDILQSLREIGMSLEEIKEYIQNRTPQLCVKMLKEEEKKIREKIKKLRKIS
                     SKMQNRINITVEGISKMNSEEIYISKKFEEYLVLSSDLSNMSNSDFMMELIDFTNYCK
                     SKNLYQGYELGVIVSNENIKNGDYLSISSFYLKIDKKIKDKKLYVKPEGMYACIKHIG
                     KYEDSYKSYEKLKKYIYENEYKIIGNSYEESILDFFCESNEDNYMTEISIQVSR"
     gene            complement(1403..3523)
                     /gene="feoB"
                     /locus_tag="PC700_001706"
     CDS             complement(1403..3523)
                     /gene="feoB"
                     /locus_tag="PC700_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDF2581018.1"
                     /translation="MGLTHNSTKMSSLKDMFDIDNKEDQFVIALAGNPNTGKSTVFNH
                     LTGLRQHTGNWPGKTVATARGNFKYKNTEYALIDLPGTYSLFALSQEEIVARDFICFG
                     NPDAVIVVCDATCLERNLNLVFQVMELTDKVILCINLIDEARKKGITIDKKLLEDSLG
                     IPVILTAARNGSGMDELLDTLNDVSFDKYKLNNKPVRYNENIENVVKSIQPELDNIIP
                     GINSRWLGLRLIDGDESIFESMSNYIDKDSIDAINEVKKKIPDNINKQKIRDEFTKIN
                     YDYAKKLSDECCSNVAKKSTDREEKVDKILTSKIFGLPIMLLLLGTILWITIEGANYP
                     STLLSNLLLGFEPSISGILNSINCPSWLNDMLVLGLYRTLAWVISVMLPPMAIFFPLF
                     TLLEDFGYLPRVAFNLDHLFKKACAHGKQCLTMCMGFGCNAAGVIGCRIIDSPRERLI
                     AILTNNFVPCNGRFPTLIAISTIFFSSVITNSFVSSVATALCITLLIILGVIITLLVS
                     YTLSKTLLKGVPSTFTLELPPYRVPQIGRTLYTSIIDRTIFVLGRAVMVAIPAGVITW
                     IFANIYIGDLSILSHVANFLDPLAKLIGLDGFILLAFILGFPANEIVVPILLMAYLAT
                     GSMIELDSFSALGQVLREHGWTYLTALNVMLFSLLHWPCATTLLTIKKETGSLKWTAL
                     GFLMPTILAFVVCFLTTTVYNLFI"
     gene            complement(3549..3776)
                     /locus_tag="PC700_001707"
     CDS             complement(3549..3776)
                     /locus_tag="PC700_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF2581019.1"
                     /translation="MKNLNDIQVKKTVKVEDILSSGNLRERMLALGLTRGAVIDVVRK
                     GPKNNLTVYKIRGSKIALRQEESSLILVSDI"
     gene            complement(4103..4543)
                     /locus_tag="PC700_001708"
     CDS             complement(4103..4543)
                     /locus_tag="PC700_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2581020.1"
                     /translation="MFKIKHFNDLSLDEFYEIAKSRYEVFACEQKIFSLNDYDDIDKS
                     SYHIFLKENGLICAYARIIPKEYSSYNDVSIGRVLVLSSHRRKGLAKQMMDCAIDFIK
                     INLHENNITLSAQTYIKNLYLSCGFKEISEVYDEAGIEHIKMRL"
     gene            4695..5684
                     /locus_tag="PC700_001709"
     CDS             4695..5684
                     /locus_tag="PC700_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HDF2581021.1"
                     /translation="MIYGGSVLNTTKKLTEAALLSSLFIVVTIIAVSTGFGYAIYLDF
                     IVPVFFCIICLKCDLKYTILSGITSLLIISLVLGNLGTAIWASQSVLLGIMCGYLINK
                     PTMVMDDLVYGSVFGVIVMVFIDIYASTLIGYSFMKEFQGYSKWIYFNGYTNFIYYLM
                     IALFPFGMVFCIYLLSLILGNKLHILDSNSLKKFLIFKNFRSLNQFLCCSKKAFYICV
                     SYLAIFEVLKLLNVKVDSVYLKTIFISITYISVYFIIRDSCMSLQNFIIAKFRKLLYA
                     RILFLIIILLLFFIFDVTVISLIIINAFLNKKINIRLSQSNIVNKQINLLIEK"
     misc_binding    5836..6088
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6160..7368
                     /locus_tag="PC700_001710"
     CDS             6160..7368
                     /locus_tag="PC700_001710"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDF2581022.1"
                     /translation="MKSIDEQMRIIMKGVDDLIDEKELREKLIKSEKEGKPMIVKLGL
                     DPSAPDIHLGHTVVLRKMKQLQDLGHQIVIIIGDFTGKIGDPTGKSKARKALTTEQVL
                     ANAKTYEEQIFKVLDKEKTIVRFNSEWLAKLNFEDVIKLAATITVARMLEREDFKKRY
                     EGQMPISVHEFFYPLMQAYDSIALEADIELGGTDQRFNLLMGRSLQREFGMESQIVIM
                     MPLIEGLDGKEKMSKSLGNYIGIDEEAGIMYQKSMEIPDELIIKYYNLVTDVHPDEVN
                     KIESQLKEGSVNPRDIKMNLAREIVTLYHGEESAKEAEERFKSVFQKGQIPEDIQTIQ
                     VKEDGFDLIEVLVSNEIVKSKSEVRRLASQGGVKVNGEKVEDLSTIVKESELVVQIGK
                     KKFVKIELVK"
     gene            complement(7429..8310)
                     /locus_tag="PC700_001711"
     CDS             complement(7429..8310)
                     /locus_tag="PC700_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HDF2581023.1"
                     /translation="MVKKRKKKTVTLTILVVIVIGIFSMAWADMSRKKEMKETQERLK
                     AERLEKERIEKEKNEPIIGAKKEAVKEYGYDAKIVWDKLNKYDYSNNGEKIVFLTFDD
                     GPSTTNTPQVLDILKRHGVRGTFFIKGDSLERKGANEILKRTFDEGNAIAHHSYTHDY
                     KKLYPGRSLNLDTFVNELNKTDEAMKKVLGKNFSSNVVRCPGGYMSWKNMEPLGNYLK
                     EKNMASIDWNALNADAEGKKKNAQELFEHAKKSSEGKEMVVLLMHDTYGKQETVNALD
                     QIITYFKDNGYQFKILI"
     gene            8696..9640
                     /locus_tag="PC700_001712"
     CDS             8696..9640
                     /locus_tag="PC700_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDF2581024.1"
                     /translation="MQKNKKEGALLKKYFGEIGLIFIAIIWGSGFVATQFALDGGLTP
                     LQIITLRFFLAAIIMNLLFFKQIRANMGKKLLKAGGILGIFLFLAFTVQTIGLMYTTP
                     SKNAFITAANVVIVPFIGFILYRRKLDKIGIISSLVALIGIGILSLEADFSINFGDFL
                     TLICSFGFAFHIFFTSEFAKDNNPMALTAIQFTVAFLMSVVVQTFAGQLKMEAELSGY
                     MGTMYLAVFSTTIGFLFQTICQKRVDGTRTAIILSTEAVFGTIFSIIILKELITAKLI
                     IGSILIFVAIITAETKLSFLKSKKVKLKDSEESSLESI"
     gene            9863..10408
                     /locus_tag="PC700_001713"
     CDS             9863..10408
                     /locus_tag="PC700_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HDF2581025.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEMVWADE
                     HRIGVAQGVDAEIIEGLRANFTGECTEVGMYLAMSRQADREGYPEVAEAYKRIAFEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKRAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRHFGK"
     gene            10499..11647
                     /locus_tag="PC700_001714"
     CDS             10499..11647
                     /locus_tag="PC700_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HDF2581026.1"
                     /translation="MKSVSVRSCKTYNYEEVKLSIEKNLEDIGGIDKFVKKGSKVLIK
                     PNLLMKKKPEEATTTHPMVVKVVCEKLLELNCDITIADSPGGPYNQNSLKSIYKASGI
                     EEVANELGIKLNYDVSDVKIHSKKAHVLKYMDIIKPIVDSDYIINLCKLKTHIMATFT
                     GGTKNLYGCIAGLKKAEIHYRFPTEELFCENVLLDICDYIKPTLTIMDGIVGMEGDGP
                     SAGTTREIGVLLASQNPYAIDVVACKIISLLPNRVATIRGAIKRGYIQEDFSDIEILG
                     EDIKDLIIKNYKIPNVSKDLRLFSVKLPKFLNEPISKLITPKPVVRFQDCIKCGKCKE
                     ACPASTIEIGTKGAIIDLSKCIRCYCCHELCPKKAIDVKRNFVFKYVK"
     gene            11700..12965
                     /locus_tag="PC700_001715"
     CDS             11700..12965
                     /locus_tag="PC700_001715"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDF2581027.1"
                     /translation="MILIKNGRVIDPKSQRDENIDLIIKENKIYKIGKFDESDEYEKI
                     IDASGNVVAPGLVDVHVHFRDPGFTYKEDIESGAKSAARGGFTTVICMANTNPIVDNE
                     DTFNYVKEKSKNACINVLQAAAITKGFEGKELVDMEALKKAGVPGFTDDGLPLMDSNL
                     IMEAMIKAKELDVPLSFHEEDPSLVGNPGVNAGKVASELGLKGASSVAEDVMVARDCM
                     LALKTGAKVDIQHISSGVSVDMVRFAKYLGANVVAEASPHHFTLTEEDVLEFGTNAKM
                     NPPLRSKWDRDKIIEGLNDGTIEIIATDHAPHSKEEKDREFIKAPSGIIGLETSLALG
                     ITNLVHKNHLSMMQLIEKMSINPAKLYNLNIGFIEEGAVADIVIFNPEEEWTVESFVS
                     KADNSPFKGKSLYGKVNYTICNGEIVYNA"
     gene            complement(13251..14483)
                     /locus_tag="PC700_001716"
     CDS             complement(13251..14483)
                     /locus_tag="PC700_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2581028.1"
                     /translation="MSLLKNSLANLKGHKLRVFVALLWIIIGITSVILVSSIGNGFQK
                     EIKKSVNNVNPNKTTISFESADNTGLTDDMSIFLKPFNAKDLEELSFVEGVERIAPSR
                     DGFNLDSVYSSQASFDKKTTYVDVGPVKKDSKINLICGRDFSLDDEKRKVILLTLQST
                     SEIFENPEDALGHGININGTIFEIIGVLDDSQQNQAGGFFGGYQDMQFTTSLVPKKAF
                     DTLMSQNSYSNEIYQLDLVSSKGYNVNEVANNVIAKLYEMHPGINGSYTTPDPTEQTA
                     YLESINSNVNKYVSIITVVAMFVGGIGVMNIMYVSVMERQREIGIRRAIGAKPRSILF
                     QFLVEAVFITVCGGILGTIVGFAATNYVSKYIGFEAIPSLNSLFYAIVATILTGVVFG
                     LIPAFKASKLDPIKAIYK"
     gene            complement(14486..15160)
                     /locus_tag="PC700_001717"
     CDS             complement(14486..15160)
                     /locus_tag="PC700_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2581029.1"
                     /translation="MLIKLENIQKYYKVGKDELHVLKSLNLEIESGEFVMIMGKSGSG
                     KTTLLNILGFLDVFDEGRYIFDGTDVTNLSENERSVFRNINIGFVFQQFNLIETLNVY
                     QNVELPLIYNKALKKSNREEIVKDKLSSVGLLDKLKQKPLQLSGGQQQRVAIARCLVN
                     DPQIIFADEPTGALDSETSREIMELLTRLNKQGKTIIMVTHDQDLTKYATKVIRLKDG
                     VFTSEV"
     gene            complement(15168..16397)
                     /locus_tag="PC700_001718"
     CDS             complement(15168..16397)
                     /locus_tag="PC700_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDF2581030.1"
                     /translation="MSFLKKFKNKRFRNNSFKSNNFRNSSFGSSGFKSKNKGFKNNPF
                     KNKRFKIYAGIAVIILLILGVLAYVDSKNTELQANENFIETYTIPENEKIFINGMVVP
                     KQTKDFNISGDYELSDVNVTNGQKVNQGDLLFTAKNPTIIAEIDSLKSQLSQYKKQKI
                     SLSDIAENKDAIASINAQITALNSQIASLDKKAYDRVTAPFDGTVYLNDQTGNPDQPV
                     SFMTIQGLEFYMKGQASEQDLPKMKIDQMVNILVFSTDQKLTGRISFISDKPSTPNTE
                     MGAQQNTLSYYDINIAFDNQEGLVNGFHLQASLEVANSSFKIPASCVLKDNKHSYVFK
                     DLDGILKKQIVDVASQNDDFAVVRGGLEQGDIIIKHPTKEMKEGDPVQGDGVTAGSNN
                     GDTTPNKKVEEVTMDVN"
     gene            complement(16509..>17184)
                     /locus_tag="PC700_001719"
     CDS             complement(16509..>17184)
                     /locus_tag="PC700_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2581031.1"
                     /translation="NNIEKFEENKEILNMWVHDVKIPIAIIKLILEQNENLINEKISD
                     DIDSEIFRIENSVEKILYLSRLEDFHKDFLIQEVNIEKIIRNIIKKYSKYFISKKIIL
                     DLNNLNFKVLSDEKWLYFIFEQLLSNSLKYSSLGDNISIYCKRTKNYLQLRVEDTGCG
                     IKKEDLNRIFNKGFTGNNGRNNSKSTGLGLYLVKELCDKLGHKIDVDSEYNQYTKFTI
                     TFKCDL"
BASE COUNT         6272 a         2256 c         2617 g         6039 t
ORIGIN      
        1 ataatagata ctaatgctaa tgatgttatt gcagtatcta atcttcttat taataaactc
       61 atttctatat attcaagttc tattaaatat tgataaaata tttctagttt aacagctttt
      121 tctctatcta tatttccata ctttaactct aatttatcta aagatttttt attacaataa
      181 ttaactttat gtttaattaa atatcttatt gtagcaccta ttacaatttt cactctaaca
      241 agcataaata taatattcca tccaaaagtt aagaggggat atctttttta tgagaggaat
      301 ggataaaata tttataactg acatctaact gatatttaag ctttaaaatc aaaaccatat
      361 tgattttcta aaataataac tacttgattt agtaaataat aaagaatata gtaactatat
      421 agacaacaaa aagaaaagga gtgtataacc cttggaaaaa cagtatttta caacaggtga
      481 atttgctaaa ttgtgtggta tatctaagca gacattaatt ttctatgaca aaataggtat
      541 tttttctcct gaatataaag ataaaaataa ttatagatat tactcagttt atcaatatga
      601 tacacttgat atattacaat cattaagaga aataggaatg agtttagagg aaataaaaga
      661 gtatatacaa aatagaactc cccagctttg tgtgaaaatg cttaaagaag aagaaaaaaa
      721 gataagagaa aaaataaaaa aactaagaaa gataagctct aagatgcaaa atagaataaa
      781 tataacagtt gaaggaattt ctaaaatgaa tagtgaagaa atttatatat ccaaaaagtt
      841 tgaagaatat ttagttctat caagcgattt gagtaatatg agtaatagtg attttatgat
      901 ggaattaata gatttcacca attattgtaa atcgaaaaat ttgtatcaag gatatgagtt
      961 gggagtaata gtaagtaatg aaaatataaa aaatggggac tatttaagta tatcaagttt
     1021 ttatttgaag atagataaaa aaataaagga taaaaagcta tatgtaaagc cagaaggaat
     1081 gtatgcttgc ataaaacata ttggaaaata tgaggattca tataaatctt acgaaaaatt
     1141 gaaaaaatat atatatgaaa atgaatataa aattattgga aattcgtatg aggagagtat
     1201 acttgacttt ttttgtgaaa gtaatgaaga taattatatg actgaaattt caattcaagt
     1261 atccagataa gaattaaaat agagatatat tcaaatgtta agtaatttta cttatcattg
     1321 aatatatctc tatgctttat aataaagtat ataaattttt aaatagtttc tctaatatat
     1381 gtctaatgtc agttaatact aattatataa ataaattgta aactgtagtt gttaaaaaac
     1441 aaacaacaaa agcaagtata gtaggcatta agaatcctaa ggcagtccat tttaaactac
     1501 cagtttcttt ttttattgtt aaaagtgttg ttgcacaagg ccaatgcagt aaactgaaca
     1561 gcataacatt taaagctgtc aaataagtcc atccatgttc tctaagaact tgacctaaag
     1621 cactaaagct atctaattct atcatagaac cagttgctag gtaagccatt aaaagtatag
     1681 gtacaactat ctcatttgct gggaatccaa gaataaaagc aagtaatata aaaccatcaa
     1741 gaccaattaa tttagctaaa gggtctaaaa agtttgcaac atgtgataat atgcttaaat
     1801 ctcctatata tatatttgca aatatccaag ttatgacacc agcaggtatg gctaccatta
     1861 cagcccttcc taaaacaaat attgttctat ctattataga ggtatataaa gttcttccta
     1921 tttgaggaac tctataagga ggaagttcta atgtaaatgt agacggaact ccttttaata
     1981 gcgtcttaga aagagtataa gaaaccaaca atgttattat aacacctaga attatcagta
     2041 aagttatgca aagtgcagtt gctacgcttg atacgaaaga gttggttata actgagctaa
     2101 aaaatattgt agatatagct atcaaagtag gaaaacgacc attacaagga acaaagttgt
     2161 ttgtaagtat agctataagt ctttcccttg gagagtcaat tatcctacaa ccaataactc
     2221 cagctgcatt acatccaaat cccatacaca tagtcaaaca ctgttttcca tgtgcacaag
     2281 cttttttaaa taggtggtct aagttaaatg caactcttgg caaatatcca aaatcctcaa
     2341 gtagggtaaa taatggaaaa aatattgcca taggaggtag cattacagag attacccatg
     2401 ctaatgttct gtataatcct aaaaccaaca tatcatttaa ccatgatgga caattaatac
     2461 tatttaatat acccgaaata cttggctcaa atccgagtaa taaattagat aataaagtgg
     2521 atggatagtt agccccttct atagtaatcc acaatatagt acctaataat aagagcatta
     2581 ttggaagccc aaatatttta gatgttaata ttttatctac tttttcttct ctatcagttg
     2641 attttttagc aacattagaa caacactcat cactcagttt tttagcataa tcatagttga
     2701 ttttagtaaa ctcatctctt attttctgtt tatttatatt gtctggaatc tttttcttta
     2761 cttcattaat agcatctata gaatctttgt ctatataatt actcatagat tcaaatatac
     2821 tttcatcgcc atcaataagt ctaagtccca accatctaga atttatacct ggtataatat
     2881 tatccaattc tggttgaatt gattttacca cattctctat attttcattg tatctaacag
     2941 gtttattatt taatttgtat ttgtcaaaag atacatcatt taaagtatcc aatagttcat
     3001 ccataccaga gccatttctt gcagcagtta aaataactgg aataccaaga gaatcctcta
     3061 aaagtttttt atcaatagta atgccttttt ttctagcttc atctattaaa ttaatacaca
     3121 aaataacttt atctgtaagt tccatcactt gaaatactaa atttaaattt ctttcaagac
     3181 atgtagcatc acatactact attactgcat ctggattacc aaaacatata aaatcacgag
     3241 ctacaatttc ttcttgtgat aaggcaaata aagaatatgt tcctggtaaa tctattaatg
     3301 catattcggt atttttatat ttaaaattac cacgagctgt agctacagtt tttcctggcc
     3361 agtttccagt atgctgtcta agccctgtaa gatgattaaa tacagtactt ttacctgtat
     3421 ttggattgcc agccaatgca attacaaact ggtcttcctt attgtctata tcaaacatat
     3481 cttttaaaga actcatttta gttgagttat gtgtcaaccc catattaata tcctcctgct
     3541 agaaattttt aaatgtcaga aactaatatt aagctacttt cttcttgtcg tagagctatc
     3601 ttactacctc tgattttata gacagttaaa ttattttttg gaccttttct aactacatct
     3661 ataacagctc ctctagtaag accaagcgct aacattcttt ctcttaaatt accactagat
     3721 aatatatctt caactttgac agtttttttc acttgaatat cgttaaggtt tttcatagta
     3781 gcacctccat gcttgaaatt tagcctgggc taatcttctt attctaaact atgcattata
     3841 ttattacctt gttactttta caaaaaaaag ataggcaaga tgcctatcat acaaagttgg
     3901 gtataattct tacttttggg gaaagtagaa tatagaataa aaattaacac tttgaatcca
     3961 tataattatt gaattttgat aataaaaaag tcagaaagca aaagcctcct gactctatgt
     4021 aaggtatttt caaataccta aatatcaatt caatatttaa tttatactta aattatatat
     4081 taacaatatt agcttgtcaa cactataatc tcattttaat atgttcaata cctgcttcgt
     4141 cgtatacttc tgagatttct ttaaaaccac aagataagta aaggttcttt atataagttt
     4201 gtgctgataa ggttatatta ttttcgtgta agttaatttt tataaaatct atagcacaat
     4261 ccatcatttg ttttgctaat ccttttcttc tatgactaga aagaactaaa actctaccaa
     4321 tggaaacatc attataagag ctgtattctt taggaattat tctagcataa gcacaaatca
     4381 atccattttc ttttaaaaat atgtgataag agcttttatc tatatcatca taatcgttta
     4441 atgaaaatat cttctgttca caagcaaata cctcatatct tgattttgct atttcataaa
     4501 attcatctaa actcaaatca ttaaagtgtt taattttaaa catagtaatt acctcctagc
     4561 atcttaaaaa actgaatatt aagatacatt ataccatttt taacataaat acctttatta
     4621 aaatttatat ttattaaaaa tatttttaaa aagtgatata ctatattaaa aacaaaactt
     4681 tagttagaaa atttatgatt tatggaggca gtgttttgaa tactactaaa aaattaactg
     4741 aagcagcttt attgtcatct ttattcatag tagtaacaat tatagctgtg agtactggat
     4801 ttggatatgc tatttactta gattttatag tgccagtatt tttttgtata atctgcttga
     4861 aatgtgattt aaagtatacc attttatctg gaattacttc acttttaata atttctctgg
     4921 tacttggaaa tctaggaact gctatttggg caagtcagag tgtattactt ggtataatgt
     4981 gtggctatct aataaataag cctacaatgg ttatggatga tttagtatat ggttcagtat
     5041 ttggggttat tgtaatggtt ttcattgata tatatgcttc aacattaata ggttatagtt
     5101 ttatgaaaga atttcaagga tatagcaaat ggatttattt taatgggtat acaaatttta
     5161 tttattattt aatgatagca ttgtttcctt ttggtatggt gttttgcata tatttattaa
     5221 gtttaatttt agggaataaa ttgcatattt tagattctaa ttctttaaaa aaatttttaa
     5281 tatttaagaa ttttagaagt ttaaaccaat ttttatgttg ctcaaaaaaa gctttttaca
     5341 tatgtgtttc ttatctagcc atttttgaag ttctaaaact gttgaatgtt aaagttgatt
     5401 ctgtttattt aaaaacaatc tttatatcaa taacctatat aagtgtctat tttatcatta
     5461 gagactcatg tatgtcacta caaaacttta taatagctaa gtttagaaaa ttattatatg
     5521 ctagaatttt atttttgata ataattttat tgctgttttt tatatttgat gtaacagtta
     5581 ttagtttaat aattataaac gcatttttaa ataagaaaat aaatataaga ttgagtcaga
     5641 gtaatatagt caataaacaa ataaatcttt tgattgaaaa ataaactttg ggtattggta
     5701 aaaaatataa aaaagtagta taatgtaaaa aaacatgtat ataataataa aaaaggttga
     5761 caaaatataa aaataataat atattatgag taagttaaga aaaatttctt gtaattgatg
     5821 aaataatctg aatatataaa tgctatgaag agaagaagta gggctagatt ttatttaaca
     5881 gagagttcca gtttggtgaa atggaataaa ttgatttagt ttgaatacat ctcagagcac
     5941 tatctctgaa agataacaac cattaagaga tatcggatta gtacacgtta tagtacacga
     6001 gtatatgtaa ttgtactcta agagattaat atcgtgagat attgatgaac taaggtggta
     6061 acacgtaagc aatgctttcg tccttttaag aggatgaaag ctttttttat tataaaaaaa
     6121 aaattataga ttatataaat attttaggag gaatgaaaaa tgaaatcgat agacgagcaa
     6181 atgcgaataa ttatgaaagg tgtagatgat ttaatagatg agaaagagtt aagggaaaag
     6241 cttattaaat cagaaaaaga aggaaagcct atgatagtaa aattgggtct agacccaagt
     6301 gctccagata tacatttagg gcatacagta gttcttagaa agatgaaaca attacaagat
     6361 ttaggtcatc aaatagtaat aataattgga gattttacag gaaaaattgg agacccaact
     6421 ggaaaatcta aagctagaaa agctttaaca acagagcaag ttcttgcaaa tgcaaaaact
     6481 tatgaagaac aaattttcaa agttttagat aaagaaaaaa ctattgtaag atttaatagc
     6541 gagtggttag ccaagttaaa ttttgaagat gtaataaaat tagcagcaac tattactgtt
     6601 gcgagaatgt tagaaagaga agattttaaa aagagatatg aaggacaaat gccaatatca
     6661 gtacatgagt tcttctaccc attaatgcaa gcatatgact ctatagcatt agaagctgat
     6721 atagaacttg gtggaactga ccaaagattc aatttactaa tgggaagatc attacaaaga
     6781 gaatttggta tggagtctca aatagtaata atgatgcctt taatagaagg attagatggt
     6841 aaagaaaaaa tgagtaagag tttaggtaac tatattggaa tagatgaaga agcgggaata
     6901 atgtaccaaa aatctatgga aattcctgat gaacttataa ttaaatacta caatttagtg
     6961 acagatgttc atcctgatga agttaataaa atagaatctc agttgaaaga gggttcagta
     7021 aatccaagag atataaagat gaacttagca agagaaatag taacattata ccatggagaa
     7081 gaatctgcta aagaagctga agaaagattt aaatctgtat tccaaaaagg tcaaatacct
     7141 gaagatatac aaacaattca agttaaggaa gatggatttg acttaatcga agtactagtt
     7201 tctaatgaga ttgttaagag taagagtgaa gttagaagat tagcttctca aggtggagta
     7261 aaggtaaatg gtgaaaaagt agaggattta agcactattg taaaagagag tgaattagtt
     7321 gttcaaatag gaaagaaaaa atttgtaaaa atcgagctag taaaatagag taaagtaaaa
     7381 gcaagtctat tgagtataaa atgtacattc aataaacttg cttttttatt atattaaaat
     7441 tttaaattga taaccattgt ctttaaaata tgttataatt tggtccaatg catttactgt
     7501 ttcttgtttt ccataagtat catgcattaa aagaacaacc atttctttgc cttcagaaga
     7561 tttctttgca tgttcaaata attcttgtgc atttttcttt ttaccttctg catcagcatt
     7621 taaagcgttc caatcaattg atgccatgtt tttctctttt aagtagtttc ctagaggttc
     7681 catatttttc catgacatat acccgcctgg gcatctaact acatttgagg aaaaattctt
     7741 tcctaagact tttttcatag cttcatcagt tttgttaagt tcatttacga atgtatcgag
     7801 atttaggctt ctgccaggat atagtttttt atagtcatgg gtataagagt gatgggctat
     7861 tgcatttcct tcgtcaaatg ttctttttaa aatttcatta gctccttttc tttcaagaga
     7921 atcaccctta ataaagaaag ttcctctaac accatgtctt ttcaagatat ctagaacttg
     7981 tggagtatta gtagtagatg gtccatcatc aaatgtaaga aagactatct tttctccatt
     8041 gtttgaatag tcgtatttgt taagtttgtc ccaaactatt tttgcgtcat atccatattc
     8101 ttttacagct tctttttttg caccaataat aggttcattt ttttcttttt caattctttc
     8161 tttttctaat ctttcagctt taagtctttc ttgagtttct ttcatttctt tttttctaga
     8221 catgtccgcc catgccatag aaaaaatacc aataactatt acaacaagta tagtaagtgt
     8281 aacagttttc ttttttcttt tttttaccat aattacagca ttcccccttt tgtcgaattg
     8341 tatcaaaaat atattataat tatatcgaag aaagtagaaa aagtaaaccg acaaagttgt
     8401 aactaagata actaaagtgt aacttatatg acattatttg cataatatta aagtatcata
     8461 tatattaaaa atatctataa aaaaatttag tataggtaaa aaaatataaa aaatataaaa
     8521 atttattaaa aaggtgttta caaaaaatga ttaatatatt actgttaaat agtggatata
     8581 aatttgagct caaaatgtat gtaaatttag atttaaaata ctgaaaaaat ttactatatt
     8641 aatttatagt aatgcttata gaaaaactct cttgctgttt agggggttta taattttgca
     8701 aaaaaataaa aaggaggggg ctttattgaa aaaatacttt ggagaaatag gactaatatt
     8761 tatagcaatt atatggggaa gtggctttgt agcaacccaa tttgcattag acggtgggct
     8821 tacacctcta caaatcataa ctttaagatt tttcttggca gctataatta tgaatttact
     8881 gttttttaaa cagattcgtg ccaatatggg taaaaaatta ttaaaagcag gagggatttt
     8941 aggaatattt ttatttctag cattcacagt acaaactatt ggacttatgt acactacacc
     9001 atctaaaaat gcatttataa cagcagctaa tgttgtaata gttccgttca taggatttat
     9061 actttataga agaaaacttg ataaaatagg aataataagt agtttggtag ctcttatagg
     9121 aattgggata ctttctttag aagctgattt ctctattaat tttggagatt ttctaacact
     9181 tatatgttca ttcggatttg catttcatat attctttaca agtgagtttg caaaagataa
     9241 caacccaatg gcattgacag ctattcaatt tacagtagcg tttttaatgt ctgttgtagt
     9301 tcaaacattt gcaggtcaat taaagatgga agctgaatta tctggttata tgggaactat
     9361 gtatctagct gtatttagta ctacaattgg atttttattt cagactatat gtcaaaaaag
     9421 agttgatgga actagaacag ctataatatt atccacagag gctgtatttg gtacaatatt
     9481 ttctataata attctaaagg aacttattac agcaaaattg attattggta gtatcttgat
     9541 atttgttgca atcataactg cagaaactaa attatctttt ttaaagagca aaaaagtgaa
     9601 acttaaagat tcagaggagt cttcattaga aagtatttaa tatgacaact atttcctaat
     9661 taatcaaata agttatgtat tatgtagaaa tttgacatca aaacttggtt gatactggtg
     9721 tttcaaagtg ataaaattaa ttaggaaata aattttattt ttagtgttta agctataaag
     9781 tctggggtat atatattctt gtagcaagtt attaaactta aaaataaaaa tttaattata
     9841 taaaattggg aggaaattaa ttatgaaaaa atttgtttgt acagtatgtg gatatataca
     9901 tgaaggagat gctgcaccag cacaatgtcc agtatgtaaa gttggagctg ataaatttga
     9961 agaaatgaaa ggcgaaatgg tttgggctga tgaacataga ataggagtag ctcaaggtgt
    10021 agatgcagaa ataatcgaag gattaagagc taactttact ggtgagtgta cagaagtagg
    10081 aatgtattta gcaatgagta gacaagctga tagagaaggt tatccagaag tagctgaagc
    10141 atataagaga atagcttttg aagaggctga acatgctgct aaatttgcag aacttcttgg
    10201 agaagttgta gttgcagata caaaagaaaa cttaagagtt agagttgatg ctgagtatgg
    10261 tgcaactgat ggaaaattaa aattagctaa gagagctaaa gaattaggat tagatgctat
    10321 acatgataca gtacatgaaa tgtgtaaaga cgaagctaga catggtaaag cattcttagg
    10381 attattaaac agacattttg gaaaataata attaaaacat agattaaaag gagaaacttt
    10441 ggtttctcct tttttaaatt atacaaataa actgttgtga tagaaggatg gagataatat
    10501 gaagtcggta tctgttagga gttgtaaaac ttacaattat gaagaagtaa agttatctat
    10561 tgaaaaaaat ttagaggata ttggtggtat agataagttt gttaaaaaag gaagcaaagt
    10621 acttataaaa ccaaatttat taatgaaaaa aaagcctgaa gaagctacaa ctacacatcc
    10681 tatggttgta aaagttgtat gtgaaaaact tttagagtta aattgtgata ttacaatagc
    10741 agatagccct ggtggtccat ataatcaaaa ttctcttaag agcatttata aggcatctgg
    10801 aattgaagaa gtagcaaatg aactgggcat taagttaaat tatgatgttt cagatgttaa
    10861 gatacatagt aaaaaagcac atgttctaaa atatatggat attattaaac caatagtaga
    10921 ttcagattat ataataaatc tttgtaaatt aaagactcat ataatggcaa cctttactgg
    10981 tggtacaaaa aacttatatg gatgcatagc aggattgaaa aaagcagaga tacattatag
    11041 atttcctaca gaagagttgt tttgcgaaaa tgtactttta gatatttgtg attatataaa
    11101 accaacattg acaattatgg atggtatagt aggtatggaa ggtgatggac cttcagctgg
    11161 tactactcga gaaataggag ttttattagc atcacaaaat ccatatgcta tagatgttgt
    11221 tgcatgtaag attataagtt tacttccaaa tagagttgct acaattagag gtgctattaa
    11281 gagaggctat atacaagaag atttttcaga tattgaaatt ttaggtgaag atataaaaga
    11341 tttaattata aaaaattaca agattccaaa tgtaagtaag gatttaagat tgttttccgt
    11401 aaagctacca aaatttttaa atgaacctat ttcaaagctt ataacaccaa agcctgtggt
    11461 aagatttcaa gattgcataa aatgtggaaa atgcaaggaa gcttgtcctg catccactat
    11521 agagataggg actaaaggtg ctattataga cttaagtaaa tgtataagat gttactgttg
    11581 ccatgaactt tgtcctaaaa aggctattga tgtcaaacgt aattttgtat ttaagtatgt
    11641 aaaataatgt ataatattat atgagtctat ataaattaaa aatataaagg agaaataata
    11701 tgattttaat taaaaatggt agagtaatag accctaaaag tcaaagagat gagaatattg
    11761 acttaattat aaaagaaaat aaaatttata aaataggcaa gtttgatgaa agtgatgaat
    11821 atgaaaaaat aatagatgct agtggtaatg tagttgctcc aggtttagtg gatgtacatg
    11881 tacactttag agaccctggg tttacatata aagaagatat agaaagtgga gctaaatctg
    11941 ctgctagagg tggatttaca actgttatat gtatggcaaa tactaatcct attgtagata
    12001 atgaagatac ttttaattat gttaaagaaa agtcaaaaaa tgcatgtata aatgttttac
    12061 aagcagcagc tataactaaa ggttttgaag gtaaagagtt agttgatatg gaagctctta
    12121 aaaaagctgg tgtaccaggg tttactgatg atggattacc tttaatggat agcaatctaa
    12181 ttatggaggc aatgattaag gcaaaagagt tggatgtacc acttagtttt catgaagaag
    12241 acccttcttt agttggaaat ccaggtgtaa atgcaggaaa ggtagcttca gaactaggat
    12301 taaaaggtgc atcaagtgta gcagaagatg ttatggtagc cagagattgt atgttggcac
    12361 taaagactgg tgctaaagtt gatatacaac atataagttc aggtgtatct gttgatatgg
    12421 ttaggtttgc aaaatactta ggtgcaaatg tagttgcaga agcttcacca catcacttta
    12481 cattgactga ggaagatgta ttggagtttg gaacaaatgc aaaaatgaat ccacctctaa
    12541 gaagtaaatg ggacagagat aagattatag agggattaaa tgatggtact attgaaataa
    12601 tagcaacgga ccatgcacct catagtaaag aagaaaaaga tagagagttt ataaaagctc
    12661 caagtggaat tattgggctt gaaacttctt tagctcttgg aataacaaat ttggtgcata
    12721 aaaatcatct tagtatgatg cagttaatag aaaaaatgtc tataaatcct gcaaagttgt
    12781 acaatttaaa tattggattt atagaagaag gggctgtagc agacattgtt atattcaatc
    12841 cagaagaaga atggacagtt gaaagctttg tttctaaagc tgataattca ccgtttaaag
    12901 gtaaatcttt atatggaaag gttaattata caatatgtaa tggggagatt gtatataacg
    12961 cctaatttaa gattattttt ttaaggcgga gtgtacaatg attgaaagta aaagattgta
    13021 tatcagcaga atgagattga ttaatcaaaa ttggaagtat tagtttgaac aattagaaca
    13081 taacatcttg gtaatttaga tatagtaagg aagagaatcc tttttatgaa agaacgcact
    13141 attatatttg gaaagattaa gttcctaatc tatcatatat aatagtgcgt tatatattta
    13201 agtatgtttt atataaacta atctaaaaat cgaaaatctt tatacaattt ctacttataa
    13261 attgccttta tagggtctag tttagaagct ttaaaggctg gaatcagacc aaatacaact
    13321 ccagtcaata tagtagctac aattgcataa aataagctat ttaagctagg tatagcttca
    13381 aaacctatat atttagaaac atagttagta gcagcaaatc caactatagt tcctaaaatt
    13441 ccaccacata ctgttataaa aacagcttca actaaaaatt gaaataatat agacctaggt
    13501 tttgctccaa tagcacgtct tataccaatt tctctttgtc tttccataac agatacatac
    13561 attatattca ttacaccaat accaccaaca aacattgcaa ctacagttat tatagataca
    13621 tatttgttta cattggaatt aatgctttca agataagctg tctgttcagt tgggtctggt
    13681 gtagtataag aaccattgat accagggtgc atctcatata atttagctat aacattgttt
    13741 gcaacttcat ttacattata gcctttagaa gaaactaagt ctaactggta aatttcattt
    13801 gaataagaat tttgactcat taaagtgtca aaagcctttt taggtactag ggaagtagta
    13861 aattgcatat cttggtaacc tccaaagaat cctccagctt ggttttgctg agaatcatct
    13921 aataccccta ttatttcaaa tatagttcca tttatgttga taccatgacc taaggcatct
    13981 tctggatttt caaaaatttc acttgtactt tgtaatgtaa gtaaaattac ttttcttttt
    14041 tcatcatcta aagaaaaatc tctaccacaa attaaattta ttttagaatc tttttttact
    14101 ggtccaacat caacatacgt agttttttta tcaaaagatg cttgtgaaga gtaaactgaa
    14161 tcaaggttaa acccatctct tgaaggagct attctttcta ctccttcaac aaatgataat
    14221 tcttctaaat cttttgcatt aaagggtttt aaaaaaatac tcatatcatc agtaagacct
    14281 gtgttatcgg ctgattcaaa cgaaatagta gttttattag ggtttacatt gtttacagat
    14341 ttcttaattt ctttttgaaa tccattacct atagaactaa caagtattac tgaagttatt
    14401 cctattataa tccagagtaa agctacaaat acacgaagct tgtgaccttt taaattggcc
    14461 aaagaatttt ttaataaact catgattaca cctccgaagt aaacacacca tctttaagtc
    14521 gtataacctt agttgcatac ttagttaaat cttgatcatg tgtaaccatt attatagtct
    14581 taccttgttt gttgagcctt gttaaaagct ccataatttc tctgctagtt tcactatcta
    14641 atgccccagt cggttcatca gcaaagatta tctgagggtc atttactagg caacgtgcaa
    14701 tggctacacg ctgttgttga ccaccagata attgaagtgg tttctgctta agcttgtcca
    14761 aaagcccaac agaacttaat ttatctttaa caatttcttc tctattagat ttttttagtg
    14821 ctttattgta aattagaggc aactctacat tttgatatac atttagagtt tctattaaat
    14881 taaattgttg gaaaacaaat ccaatattaa tatttctaaa aacagaacgt tcattttcac
    14941 tcaaattagt tacatctgtt ccatcaaata tatatcttcc ttcatcaaat acatctagaa
    15001 aacctaagat attaagtaaa gtagttttac cactaccaga cttacccatt atcatgacaa
    15061 attctccaga ttcaatttct aaatttaagg attttaaaac atgtaattca tctttcccta
    15121 ctttgtaata tttttgaata ttttctaatt taattaacat atgtaaatta attcacatcc
    15181 atagtaactt cttctacttt tttgtttgga gtagtatctc cattattaga accagcagtt
    15241 acgccatcgc cttgaactgg gtctccttct ttcatttctt tagtagggtg ttttattatt
    15301 atatcgcctt gttcaagacc gcctctaact acagcaaaat catcattttg acttgctaca
    15361 tctacaattt gctttttcaa tataccatct aaatccttaa atacatatga gtgtttgttg
    15421 tctttaagta cacaagatgc tggtatttta aagcttgaat tagctacttc aagtgatgct
    15481 tgaagatgga atccatttac taatccttct tgattatcaa atgctatgtt tatatcataa
    15541 taagatagtg tattctgctg agctcccatt tcagtatttg gagtagatgg tttatcagat
    15601 ataaaactta ttctaccagt aagcttttgg tcagttgaaa aaactagtat attgaccatt
    15661 tggtcaatct tcattttagg taaatcttgt tcacttgctt gtcctttcat ataaaactct
    15721 agaccttgaa tagtcataaa tgaaactggt tggtctggat ttccagtttg gtcattcaag
    15781 tatactgtgc catcaaaagg agcagtaact ctgtcataag cttttttatc cagagaagct
    15841 atctgagagt ttaaagcagt tatctgtgca tttatagaag caatagcatc tttattttca
    15901 gcaatatcag aaagtgatat tttttgtttt ttgtattgac taagttgtga ttttaaacta
    15961 tctatttctg ctattatagt tggattttta gcagtaaaaa gaaggtctcc ttgatttact
    16021 ttttgaccat ttgtaacatt aacatcactt aactcataat ctccagatat attgaagtcc
    16081 ttagtttgct taggaacaac catcccattt ataaatattt tttcattttc aggaatagta
    16141 tatgtttcga taaaattttc attagcttgt aattcagtgt ttttcgaatc aacataagca
    16201 agaactccta gaattaaaag tatgattact gctattcctg catatatttt gaatctctta
    16261 tttttaaagg gattgttttt aaaaccttta tttttagact tgaagccact acttccaaaa
    16321 ctgctatttc taaagttgtt acttttaaaa ctgttatttc taaatctttt atttttaaat
    16381 ttttttagaa aagacatgtc ttcctccttt ttataaaaac tacttaatta tatactggga
    16441 tgtacatata gaattactat aagaagttga atataagtta atgaaaataa actatcaata
    16501 ataagatact ataaatcaca tttgaatgta atagtaaatt ttgtatattg gttgtactct
    16561 gaatctacat caattttatg accaagctta tcacaaagtt cttttactaa ataaagccca
    16621 agacctgtag atttagaatt gtttcttcca ttattacctg taaatccctt attgaatatt
    16681 cgatttaaat cttccttttt tataccacaa ccagtatctt ccactcttaa ttgtaaataa
    16741 tttttggttc ttttgcaata gatagatata ttatctccta atgaggagta ttttagggaa
    16801 ttactcaata attgctcgaa tatgaagtat agccattttt catcagataa aaccttaaaa
    16861 ttcaaattat ttaagtctaa aataattttt tttgaaataa aatattttga gtattttttt
    16921 ataatatttc taatgatttt ttcaatattt acctcttgaa ttaaaaaatc cttatgaaaa
    16981 tcttcgagtc ttgataaata taaaattttt tcaacggagt tttcaattct aaatatttca
    17041 ctatctatgt catctgaaat tttttcatta attaaatttt cattttgttc aagaatgagt
    17101 tttattatag ctataggtat ctttacatcg tgtacccaca tatttaaaat ctctttgttt
    17161 tcttcaaatt tctcgatatt atta
//
LOCUS       DAMJWP010000061        17083 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.063, whole genome shotgun sequence.
ACCESSION   DAMJWP010000061 DAMJWP010000000
VERSION     DAMJWP010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17083
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.063"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            146..913
                     /locus_tag="PC700_001720"
     CDS             146..913
                     /locus_tag="PC700_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDF2581032.1"
                     /translation="MGEIINALDRALDIILLLYHEKREMGITEISKAMGVYKSTVHRT
                     LVTLENKGFVIQNAENGKYWLGINLYAIGMVVGEKMSLTEIVKPYTKKLNQEFNEVVN
                     VSILEERAQDSPRSIIIHKEYGSNQLLSVNPSVGSSSECYCSAVGKCLMAFNDSIDFE
                     KYRKTPIHKYTEHTIDNWDDMMLFLAKIKEQGYAIDDEELEHGLTCIGAPILDKNNKA
                     IAAISLSGPTIRMREGDFEYKIKRVIETAKSISELFR"
     gene            1196..1393
                     /locus_tag="PC700_001721"
     CDS             1196..1393
                     /locus_tag="PC700_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2581033.1"
                     /translation="MEKVKLSVERCKGCYLCIEACKKDAISLSDYTNKKGFVTIKVDE
                     EKCIQCGACYTMCPDLVFEIL"
     gene            1422..2498
                     /gene="vorB"
                     /locus_tag="PC700_001722"
     CDS             1422..2498
                     /gene="vorB"
                     /locus_tag="PC700_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HDF2581034.1"
                     /translation="MQSLLIKGNEALAEGAIRGGCRFYAGYPITPQSEILEMMAWRQK
                     EVGGVFIQGESEIASVNMVMGASALGARAMTSSSGPGFSLKQEGIAYWVSAGLPAVVA
                     CVQRYGIGDGFIGAGQDNYWQAVKGGGNGDYHLIVLAPNSVQENMDMAYESFELAEKY
                     MHPVLILSDATIGQMVEPCIAPPMKEYDMDKAPWAAKGTPVGEKNKVITDITYFEDQD
                     TWQKGYIEKMRKVQEDEQRWEEIEIEDAEVVFVAYGISSRIAEGAVRDARAKGMKLGL
                     IRPKTLWPYPRKAFKKLSDSLKGLVTVEMNMMAQMKEDVIIACDNRFPVYSVATAQYM
                     AEVNQLIDFAQQIIDGKAKQEEVF"
     gene            2501..3220
                     /locus_tag="PC700_001723"
     CDS             2501..3220
                     /locus_tag="PC700_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HDF2581035.1"
                     /translation="MAKKAPAIMMCENMFCGGCGHSIVDRIIGEVLTEMDICQDTIIC
                     SDIGCNHMFQFSMNVDVVVPPHGKMGAAMAAMKRVRPDKYIFTIAGDGGAYAIGLGET
                     MSAATRNDNVIFLVMNNNMFAMTGGQMAPTTIEGQYTASTPAGRNYADHGSTFDVVKV
                     MGNLDIAYLARGTITNPKEINTTKRYIKKALEKQKEKKGFCLIEVLVPCPTNWGLKPV
                     DAMKRIENELVPKYPLGEFID"
     gene            3233..3772
                     /locus_tag="PC700_001724"
     CDS             3233..3772
                     /locus_tag="PC700_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HDF2581036.1"
                     /translation="MLNEIICAGFGGQGVLTAGKIIMYVAYKAEKKITWFPSYGNEMR
                     GGSANCNVVVSDEKIANPCVKNPDILLGMNSVSVDKYESLMKKNGYMFVNESLVSKDR
                     KYREDINVIKVPVTEIAQNLGNEKAANICMLGAMVKSLDLFTKEEFESGMCEYFENQG
                     KGKFNSKNVEAFEAGYDYE"
     gene            3929..4987
                     /gene="buk"
                     /locus_tag="PC700_001725"
     CDS             3929..4987
                     /gene="buk"
                     /locus_tag="PC700_001725"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HDF2581037.1"
                     /translation="MKVLVINPGGTSTKISVFQDENEILKKNITHTREDLKNFSKVFD
                     EYDYRKQLIVDILSSENHSINSFDAVVGRGGLMKAIKGGTYTVSEEMIEDMRNEINGE
                     HASNLGALLAKTIADEIGVQSFVVDPVSVDEFDDVSRITGISDIEKASWLHALNHKAV
                     SRKIAQKLGGKYEDYNFIVAHLGSGISIVAHKQGKMIDGSGGRSDGPFSPERSGGLLA
                     YPLIELCYSGKYTHDEMVAKVSSIGGMYDYLGTKDMIEIENRIKEGDEKAKLIMDAFI
                     YQVAKEISMYGASLSGNVDRVILTGGISHSEMVVKGVAEKVSYLAPIEIVPGEMEMEA
                     LALGALRVLNGEEIAKTY"
     gene            5005..5898
                     /locus_tag="PC700_001726"
     CDS             5005..5898
                     /locus_tag="PC700_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HDF2581038.1"
                     /translation="MLKDFNNLLEEAKKLEPVIVSVAVAEDKEVLKAVKSATDLNIIK
                     PILVGDKDKIESISREIGLKGHEIIGCQNPDESVKKAVEIVKNKEAKVLMKGLVNTSV
                     YMRAILNKETGLRTGRLLSLLAVYELPQYHKLLYCTDSGINVSPNLNQKKDIMTNALL
                     TMKSIGMENPKTAILTANEMVDPKIQSTVDANLLVEMEKSGEIPKCIVEGPIAFDVAF
                     DAHAAEHKGIKSNIAGDVDLLVFPNIEAGNILGKSWLRFNKAKWAGIVLGATNPIILG
                     SRSDTAEIKINSIALACLASQ"
     gene            5928..7136
                     /locus_tag="PC700_001727"
     CDS             5928..7136
                     /locus_tag="PC700_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2581039.1"
                     /translation="MKYAKRMDMVKASAIRASQKKIAQKVASGGTVISFAAGLPDPNL
                     FPIEEISEITQKVLNERGKFALAYGPTKGEDELLDLLVKRMKEEENIDTKAENIIITT
                     GSQQGIALSAMILLEKGDIVVTENPSYLGAINAFRPYECDFLGVDTDDEGMVTDDLDK
                     LLSENSNIKVIYVIPTFQNPTGKTWSLQRRKDFMEVVNKYDVIVIEDNPYGEIRFTPE
                     PLPTLKSLDTKDKVLYLGSFSKVLCPGFRVAWMCGNVDIIDKAELLKQGIDLQSNQFS
                     QVQVIEFLKKYNLNEHIEKIRAEYKKRCLLMLDAMKKHFPEEAKYTEPDGGMFIWVEL
                     PESINVDDLLDKAIDAGVAYVSGESFFANNGPKNTMRLNYTTMSEEQIVKGVEILAEV
                     IKKELNYACE"
     gene            7160..8422
                     /locus_tag="PC700_001728"
     CDS             7160..8422
                     /locus_tag="PC700_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2581040.1"
                     /translation="MGEKENGIKFGKLMFQLVIIAISWELVYIIPFIQYTLYDPILKA
                     LECTNTQLGFLLTIYGLGNIFGAPIGGWLADRFDYRKIILGSVFLNGVVSFLFAFNMN
                     YPFAVATWVGCAITSLVMNYPSMVKILRVIGKDNQGKVYGFNEAMVGVSGVVMGAIFL
                     YIYTCFSTPVLGMRWVMISLGILSIVMCPVLWFVIKDVDIREDKEEEASEKMSAGDFM
                     TVLKSPNTWLVGISIFCVYSFTVTMSYFTPYITSVLGGSVALSGALAIIRQHGLKLFG
                     APFGGYCADKVKSPTKVLLPIYVFGIAVIILFLVLPASTPMTIFIALTFVVGILGYMG
                     KGIYYAVQDEVKVPVKYSATTIGIAAALGFSPDVFQFALIGHWIDTYGNKGYTYTFIF
                     QIAILIIGILSCLYILKVKKKRESKLEA"
     gene            8998..10461
                     /locus_tag="PC700_001729"
     CDS             8998..10461
                     /locus_tag="PC700_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HDF2581041.1"
                     /translation="MTDLVNTINGIVWSPVLVAMCLCIGLYFSMKLRFFQIKDVKEMF
                     TLLLEGDSSHVGISSFQGFATSMAGRIGTGNIAGVAVAIAMGGPGALVWMCIMATLGS
                     ATAFVESTLAQIYKDEHDGQYRGGPPYYFEKGLGWKKCSILFSVVAIISYTFFMPGTQ
                     SNTLYLAMNQAFGFSKMTIAVTSSIALGIIIFGGIKRIGLFAEKVVPIMGGAYLIMTL
                     VIIIANINLVPGAIMTMFKSAFGFGSVFGGMLGSAISWGVKRGIYSNEAGEGSGTYGA
                     AAAEVSHPAKQGLVQAFSVYVDTLLICMATGIMIIITGMYNVTDVYTTLPGVEPGPQY
                     VQSAINTLHPQLGDIFIAIVLLLFTFTTLLAFSYMMETNVSYLVTRIKAEKHEKHIIN
                     VCRFMLIICAAMCCFIEPLTSWALGDICIGVLTYINLVGIFTLKKPAIKAYYDYQRQK
                     KMGVKRSDRTFNPIELDIRNAEFWENRYLKEDKHELN"
     gene            10517..11653
                     /locus_tag="PC700_001730"
     CDS             10517..11653
                     /locus_tag="PC700_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2581042.1"
                     /translation="MKERIIQVADSKKEKILGLCQSLYDEPEIALQEYKSAKKISEFL
                     REEGFDVEENLAGMATAFKATKKNGDGPKIAFIAEYDALPGNGHACGHHLIASMGVGA
                     GIALSSILDTYKGEVSIIGTPAEETGDGKPYLIEHGVFDGYDAAMMIHPNSKTCVTPE
                     IIAIGGLDFIFTGKASHAGAKPYNGINALDAVVLLYNNINALRQQLVDGTRIHGIILE
                     AGTAANVIPDMGKVRLEIRAKEQNYFDEVVEKVKNCARGAAIATGCELEFYHFEPTCQ
                     GLNENKVLVDIFTKIMEEFGIYEDEQVMLGSTDMGNLSQIMPCIHPLMKFSENGEELH
                     TKEFLEASINSYAKDRVIDGIKILALTGFNLFENPELLKKMKEE"
     gene            11889..13004
                     /locus_tag="PC700_001731"
     CDS             11889..13004
                     /locus_tag="PC700_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HDF2581043.1"
                     /translation="METLEVRNIELSGTNYEIGYRLGELVANMPEIIEGQINKSNLVS
                     KKEEKEMIELFDKYCPGLNEELQGFADAIQVNCNQILYYTMTYLKPGCSQVALTPELT
                     ENGHVLFARNFDFSHNMEDFVLCKTKVNGKYAHIGTTVMQFGRGEGMNECGLGVSQSS
                     CGIPVGNSDGLRKPAIVGLQFWAVIRYLLENCKDVDEALEYLKDMPIAYNINLLLADK
                     SGNIALVETLDGKKEVNIINSLESKREYFLHSTNHTHIDKLHKLEPQSMKNSIHRYKL
                     IKEYINKAKKIGEKELMNLLSLKYPNGLSCNYYNDFFGTLKSIVMDLNIGKFNILWGG
                     LENKWESYYLKNDIKSITQRININIEKAPSDFFDFIE"
     gene            13156..14025
                     /locus_tag="PC700_001732"
     CDS             13156..14025
                     /locus_tag="PC700_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2581044.1"
                     /translation="MDYLKYFEKAVMYIENNLHENITVNDVAKETGYSYYHLTRLFKS
                     MFGESVGSYIKKRRLVSSTKELLYSDKKVIDIAIGSGFESSEAFSRAFKSIYKVSPIE
                     YRKNRIDVFVGKKKKLELDFMRHLVGNITIKPVIKEIEKIKVIGIKDKVILENNSLPD
                     LWEKFRKVHHIVPNTLPSKRVFGICEAISEIHLVSESMEFNEIIGLEVNSYDVIPNSF
                     VSKTIKEGKYAIFTHTGSLDNLDKTYEYIWGTWFLSSKEELDIRDDFEVYDERFLGPS
                     NVNSQIDIYIPIK"
     gene            14217..15107
                     /locus_tag="PC700_001733"
     CDS             14217..15107
                     /locus_tag="PC700_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2581045.1"
                     /translation="MAKLTLEHIKGNTYYIPLPTIVGVYVDGKDAILIDSGNNKDTAR
                     QVLRLLEEHNLSPKLIINTHSNADHIGGNAYLKNQTKCKIATTKIEGYFTENPILESA
                     FLYGGYPSKALKNKFLLAKESEVDYIIPSNGKIVDTELEAISLPGHYFEMIGVRTPDN
                     VLFVADSLTPENIITKYHFFFLLDIDSQFKTLEKLRMLEADFFVPSHSVKTTDIKSLI
                     NINKKKMEEIIDNIKKVCYEPVMIDEVIEKMCDLYNVKLDANQYVLVGSTIRSYITYL
                     YENNMVEYIFDGGKMMIKAV"
     gene            15325..17079
                     /locus_tag="PC700_001734"
     CDS             15325..17079
                     /locus_tag="PC700_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp23"
                     /protein_id="HDF2581046.1"
                     /translation="MYIMNKKFKKFSKKLSILILVLTMFLFNNLTANAQIKEDSIIGI
                     DRYETAGLIADRQTYDTVILVNGDKNLSDGLSSSGLAGAINAPILLTKKSEIPKATLN
                     RLDNKSLNVVKKVYIIGGYNTIENSVEKDIKRKGIQVERINGNNRIETSYNVAKKINE
                     VSSVKEVMLTNGFVGEADAMSIAPVVAKNKGAIILTDGKSVPFKTEGLNVYAIGGKSA
                     INEDLVNETKATRIGGNDRFETNKKVMEKFYNGATDFYITNGYQLVDALTLSPLAKEK
                     PIVLVADGSNKGILKGAKSITKVGGIDANIYKQCLDMIKYNNMDVTPNILKHLTSVEG
                     NEVSEILIESDNLGVVVEKTNTNKFEFDYVSVTNEKNCTFSVYKENSSNNVKNGKLVV
                     SAKKKIDKKDSDLQDMNGDNMINANKDRMVNVIKIGVPDKMYSNFKVDVESGSVELYN
                     IKGGATVDVNDGIANIVDNNVTNPFNINTNSGISGVTAETISSEIKFRANNGIVSVTA
                     TNINGNISLFGSDNKGTFDGIFKLNLKKEPSNLHLKLVGNGINKLPEGWSKDYILGNG
                     QPIIEVKNNGINNISIGE"
BASE COUNT         6669 a         1823 c         3277 g         5314 t
ORIGIN      
        1 ttttgttttt attttacaaa ttaagtataa tatttttgtt ttataaaata taattaagat
       61 aaatagataa ttttgtaatt ttaatatata atatttagta gagtgtggga gtagcactta
      121 atatttgcag gaggagtttt tgtatatggg agaaataata aatgcattag atagagccct
      181 agacataatt ttgctgttat atcatgaaaa gagagaaatg ggaataactg aaataagtaa
      241 ggctatggga gtatataaaa gtactgtaca tagaactttg gttactcttg aaaacaaagg
      301 ttttgttata caaaatgcag agaatggtaa gtattggcta ggaataaatt tatatgcaat
      361 aggtatggtt gtgggagaaa aaatgtcatt gactgaaata gtaaaaccat atacgaaaaa
      421 attaaatcaa gaatttaacg aagtagttaa tgtatctata ttagaagaaa gagctcaaga
      481 ttctccaaga agtatcataa tacataaaga gtatggttca aatcaattac tttcagtaaa
      541 tccatccgtt ggttcaagta gtgaatgtta ttgttcagca gttggtaaat gtcttatggc
      601 atttaatgat tctatagatt ttgaaaaata tagaaaaaca ccaatacaca agtatacaga
      661 acatacaatt gataattggg atgatatgat gcttttttta gcaaaaataa aagaacaagg
      721 atatgctata gatgatgaag aactagaaca tggactaact tgtattggag cacctatctt
      781 agataaaaat aataaagcta ttgcagccat aagtttgtca ggtccaacta taagaatgag
      841 agaaggggac tttgagtata agattaaaag agttatagaa actgcaaaaa gtatatctga
      901 actttttaga tgatttttaa tctcgataat aaaaataggc tatatttaaa ttgttatttg
      961 taaatatcag tttaaatgta gcctattttt ctttaatcta aaattaaata ttcttaaaat
     1021 ttaaaatgtt attgaaatat ataagcgaat gtgttatctt ttaattaagc aataaaaata
     1081 gaacaaggtt ctatactata gaacaaaatt gatgcatata tagtatttat acaaaatata
     1141 aatattatat atttaaaatt aagtaaaaat attacataaa ggggagattt gagaaatgga
     1201 aaaagtcaag ttgagtgttg aaagatgtaa gggatgctat ctttgtattg aagcttgcaa
     1261 aaaagatgca ataagtcttt ctgactacac aaataaaaaa ggctttgtaa ctattaaagt
     1321 cgatgaagaa aaatgtattc aatgtggcgc ttgttataca atgtgccctg atttagtatt
     1381 tgagatatta taaattattt ttattagagg ggagaaaagg tatgcaaagt ttattaatta
     1441 aaggaaatga agctttagca gaaggtgcta tacgtggtgg ctgcagattt tatgcaggtt
     1501 atccaataac accacaatca gaaatattag aaatgatggc atggagacaa aaagaagttg
     1561 gaggagtatt tatccaaggt gaatcagaaa tagccagtgt aaatatggtt atgggagcaa
     1621 gtgcacttgg agcaagagct atgacttctt catctggtcc aggattttca ttaaaacaag
     1681 aaggtattgc atattgggta tcagctggtt tgccagcagt tgtagcatgt gtgcaaagat
     1741 atggtatagg tgatggattt ataggagctg gtcaagataa ttattggcaa gcagtaaaag
     1801 gcggtggaaa tggagattat catttaatcg tgttagcgcc taacagtgtt caagaaaata
     1861 tggatatggc atatgaatct tttgaattgg ctgaaaaata tatgcatcca gtacttatac
     1921 tttctgatgc tacaataggt caaatggtag aaccttgtat agcaccacca atgaaagaat
     1981 acgatatgga taaagctcca tgggctgcaa aaggaacacc tgtaggagaa aaaaataaag
     2041 taataactga tataacatat tttgaagacc aagatacatg gcaaaaaggg tatatagaaa
     2101 aaatgagaaa agttcaagaa gatgaacaaa gatgggaaga gatagaaata gaagatgctg
     2161 aagtggtatt tgtggcatac ggtatttcat caagaatagc agaaggtgca gttagagatg
     2221 ctagagcaaa aggaatgaaa ttaggtctaa taagaccaaa aacattgtgg ccttacccaa
     2281 gaaaagcatt taagaaatta agtgattcat tgaagggact tgtaacagtt gaaatgaaca
     2341 tgatggcaca gatgaaggaa gatgtaatta tagcatgtga taatagattt ccagtatatt
     2401 cagtagcaac agctcaatat atggcagaag taaatcaatt aatagatttt gctcaacaga
     2461 ttatagatgg aaaagctaag caagaggagg tattttaatt atggctaaga aagcaccagc
     2521 aataatgatg tgtgaaaata tgttttgtgg aggctgtgga cactctatag tcgatagaat
     2581 tattggagaa gtattaacag aaatggatat atgtcaagat acaattatat gttcagatat
     2641 aggttgtaat catatgtttc aatttagtat gaatgtagat gttgtcgttc cacctcatgg
     2701 taaaatgggt gcagcaatgg cagctatgaa aagagttaga ccagataaat atatatttac
     2761 aatagctgga gatggtggag cttatgcgat agggcttggg gaaactatgt cagctgctac
     2821 tagaaatgat aatgttatat tccttgttat gaacaataat atgtttgcaa tgacaggagg
     2881 gcaaatggct ccaactacta tagaaggtca atatacagct tctactccag cgggtagaaa
     2941 ttacgcagat catggttcaa catttgatgt tgtaaaagtt atgggaaatt tagatatagc
     3001 atatctagca agaggaacaa taactaaccc taaagaaata aatacaacaa aaagatacat
     3061 caaaaaagct ctagaaaaac aaaaagaaaa gaaaggtttt tgcttaatag aagtgcttgt
     3121 accatgtcca acaaactggg gattaaagcc agtagatgct atgaagagaa ttgaaaatga
     3181 acttgttcca aagtatccac ttggagaatt tattgattag ggaggaaata atatgttgaa
     3241 tgaaattata tgtgctggtt ttggaggaca gggagttctt acagcaggaa aaattataat
     3301 gtatgttgca tataaggcag aaaagaaaat tacatggttc ccatcttatg gaaatgagat
     3361 gaggggagga agtgcaaatt gcaatgttgt tgtaagtgat gaaaaaattg caaatccttg
     3421 tgtaaaaaat ccagatatac tattgggaat gaacagcgta tcagtagata agtatgaaag
     3481 tttaatgaaa aaaaatggtt atatgtttgt caatgaatca ctagtaagta aggatagaaa
     3541 gtacagagaa gacattaatg ttataaaagt acctgttact gaaatagcac aaaatttagg
     3601 caatgaaaaa gctgctaaca tttgcatgtt aggtgctatg gtaaagagtt tggatttatt
     3661 tacaaaagaa gaatttgaaa gtggtatgtg tgaatacttt gaaaatcaag gcaaaggtaa
     3721 attcaattct aaaaatgtag aagcatttga agcaggatac gactacgaat gaatatttta
     3781 aatatttaaa ataattattg aaatacttat ttttatatgt tagtatttaa ttaataaaat
     3841 atagaacgtc gttctggaca ataggacgaa gtctttaggc cggaatacaa gaacgacgtt
     3901 ctgtatagta gaactagggg gtgttaaatt gaaagtttta gtaataaatc ctggtggtac
     3961 atccacaaaa atttctgttt tccaagatga aaatgaaatt ttgaaaaaaa atataaccca
     4021 tacaagagaa gatttaaaaa atttttctaa agtatttgat gaatatgatt atagaaaaca
     4081 actgatagta gatattttaa gtagtgagaa tcatagtata aattcttttg acgcagtagt
     4141 tggtcgtggt ggacttatga aggctataaa aggtggtacc tatactgtga gtgaagaaat
     4201 gattgaggat atgagaaatg aaataaatgg ggaacatgca tcaaatttag gtgcattatt
     4261 agctaaaact atagctgatg aaataggagt acaatcattt gtagtagacc cagtttctgt
     4321 agatgaattt gatgatgtat cgaggattac aggaatatca gacatagaaa aagcaagttg
     4381 gttacatgca ttaaaccata aagctgtttc aagaaaaatt gcacaaaaac tagggggaaa
     4441 atacgaggat tataacttta ttgtagctca tttaggttca ggaatatcga ttgtagcaca
     4501 taaacaaggc aaaatgattg atggaagtgg tggtaggtct gatggtccat tttctccaga
     4561 aaggagtggt ggattacttg catatcctct tatagaattg tgttactccg gaaaatatac
     4621 tcatgatgaa atggtcgcaa aagtaagttc gattggggga atgtacgatt atttaggaac
     4681 aaaggacatg atagaaattg aaaacagaat caaggagggt gatgaaaagg caaaacttat
     4741 aatggatgca tttatttatc aagtagcaaa agaaatttct atgtatggag cttcactttc
     4801 agggaatgta gatagagtta tcttgacagg agggatatct cactcagaaa tggtagtcaa
     4861 aggagtagca gaaaaagttt catatctagc tccaatagag atagtaccag gagagatgga
     4921 aatggaggca cttgctctag gagcacttag agtacttaat ggagaagaaa tagcaaaaac
     4981 ttattaatat ttaggagaaa gagtatgtta aaagatttta ataatttatt agaagaggct
     5041 aaaaagcttg aacctgttat tgtaagtgtt gctgttgcag aagacaaaga agtattaaag
     5101 gctgtgaaat cagcaacaga tttaaacatt ataaagccaa ttcttgtagg agacaaggat
     5161 aaaatagaaa gtatatcaag agaaattgga ttaaaaggtc atgaaataat aggttgtcaa
     5221 aatcccgatg aatcagttaa aaaagctgta gagatagtta aaaataagga agctaaagtt
     5281 cttatgaagg gattagtaaa tacaagtgtc tatatgagag ctatattaaa taaagaaacg
     5341 ggtcttagaa caggaagatt attaagtttg cttgcagtat atgaattacc tcaatatcat
     5401 aaacttcttt attgtactga tagtggaata aatgtatcgc caaacttaaa tcaaaaaaaa
     5461 gatataatga caaatgcact tcttactatg aaaagtatag gcatggaaaa tcctaaaact
     5521 gcgatactta ctgcaaatga aatggtagac cctaagatac aatctacagt ggatgccaac
     5581 ctacttgtag agatggaaaa atcgggagaa ataccaaagt gtatagtgga aggtccaata
     5641 gcatttgatg tagcatttga tgcacatgca gcagaacata aaggtattaa aagtaatatt
     5701 gctggtgatg ttgatttatt agtatttcca aatatagaag cagggaatat attaggaaag
     5761 tcatggctta gatttaataa agctaagtgg gcaggtatag ttttaggagc aacaaatccg
     5821 ataattttag gttcacgttc tgacacagct gaaataaaaa taaattcaat agcattagcg
     5881 tgcctagcat cacaataaga aattattatt aaggaggaga aagtattatg aaatatgcaa
     5941 aaagaatgga tatggtaaaa gcttcagcta taagagcttc tcaaaagaaa atagctcaga
     6001 aggtggcaag tggtggaact gttatatctt ttgctgcagg gcttcctgac ccaaatttat
     6061 ttccaataga agagataagt gaaataactc aaaaagtact taatgaaagg ggaaaatttg
     6121 ccctagcata tggacctact aaaggtgaag atgagttgtt agatctatta gtaaaaagaa
     6181 tgaaagaaga agaaaatatt gatacaaaag cagaaaatat tataataact actgggtcac
     6241 aacaagggat agcattaagt gctatgattt tactagaaaa aggagatata gtagttacag
     6301 aaaatccaag ttacttaggt gcaataaatg catttagacc ttatgaatgt gattttttag
     6361 gagtagatac tgatgatgaa ggaatggtaa cagatgattt agataaatta ttatctgaaa
     6421 attctaacat aaaggtaatc tatgtaatac caacatttca aaatcctaca ggaaaaacat
     6481 ggtctttaca aagaagaaaa gattttatgg aagtcgtgaa taagtatgat gtcatagtaa
     6541 tagaagataa tccttatgga gaaataagat ttacaccaga gcctctacct acacttaaat
     6601 ctcttgatac taaagataaa gtattatact taggttcatt ttcaaaagta ttatgtccag
     6661 ggtttagagt tgcttggatg tgtggtaatg tagacatcat agataaggca gaacttttaa
     6721 aacaaggtat agatttacag tcaaatcaat tttcgcaagt acaagtaata gaatttttaa
     6781 agaaatacaa cttaaatgaa catatagaaa aaataagagc agagtataag aaaagatgtc
     6841 tgctaatgtt agatgctatg aagaaacatt tcccagagga agctaaatat acagaaccag
     6901 atggaggaat gtttatatgg gttgaacttc ctgaaagtat aaatgtagat gatttattgg
     6961 ataaagccat tgatgctgga gttgcttatg tttctggaga gtccttcttt gcaaataatg
     7021 gtccaaaaaa tactatgaga cttaactata caacaatgtc agaagaacaa atcgttaaag
     7081 gtgttgagat acttgcagaa gttataaaaa aagaactcaa ttatgcttgt gaataaatat
     7141 aaaaagtgag ggtttaaata tgggagaaaa agaaaacggt ataaagtttg gaaagttaat
     7201 gtttcaactt gttattatag caatatcttg ggaattagtt tatattatac catttatcca
     7261 atatacatta tatgatccaa tcctaaaagc attagaatgt acaaacactc aactaggttt
     7321 tctacttaca atttatgggt taggtaatat atttggagct cctataggag gctggttagc
     7381 agatagattt gattatagaa agataatttt aggttctgta tttcttaatg gagtagtatc
     7441 atttttattt gcatttaata tgaactatcc atttgcagtt gctacttggg taggttgtgc
     7501 aatcacatca ttagttatga actatccttc tatggtaaaa atacttagag ttataggaaa
     7561 agataatcaa ggtaaagttt atggattcaa tgaagctatg gttggagttt ctggcgtagt
     7621 aatgggagca atattcttat atatttatac atgcttttca actcctgtat tgggtatgag
     7681 atgggtaatg atatctttag gaatattatc aatagtgatg tgtccagtat tatggtttgt
     7741 aattaaagat gtagatatca gagaagacaa agaagaagaa gcaagtgaaa aaatgtctgc
     7801 tggcgatttt atgacagtac taaaatcacc aaacacttgg ctagtaggaa taagcatatt
     7861 ctgtgtatat tcattcacag ttacaatgtc ttattttaca ccatatataa catcagtact
     7921 tggaggttct gtggcattgt ctggggcatt agcaataata agacaacatg gtctaaaatt
     7981 gtttggagca ccatttggag gatattgtgc agataaagta aaatcaccta caaaagtact
     8041 actaccaata tatgtatttg gtatagcagt aataatttta ttcctagtac tacctgcatc
     8101 tactccaatg actatcttta tagctttaac atttgtagta ggtatattag gttacatggg
     8161 aaaaggtata tactatgcag ttcaagatga agtaaaagtt ccagttaaat attcagcaac
     8221 tactataggt atagctgcag ctttaggatt ttcaccagat gtattccaat ttgcattaat
     8281 aggacactgg atagatactt atggcaataa aggttacaca tatacattta tattccagat
     8341 agcaattcta ataattggta tactatcttg tttatatata ttaaaagtta agaagaaaag
     8401 agaaagtaag ttagaagcat aaatattgta aaaataccaa attattaagt tttatagcaa
     8461 tacttaaaat ttaaagtgca acacaccaaa atgttatcaa gttttctagg ttttagtata
     8521 aagttacccc taaaggtctg gaattgagta gcaattatca tctatttata tatttacata
     8581 aacctattta atttaaatta aaagaatttc aaaaataggt ggtaattgta ctcaatttta
     8641 cacctcataa taaatacgaa aatttaaaat ctaactatat actattcaat tatatattaa
     8701 taattgtata tggtactatt tatatagtaa cagtttcagg cttttcatta catgtattcc
     8761 aatttatatt gtttgattat ttgtcaaata catatcgtta aaaagtttat aaatttacgt
     8821 ttatatatcg attaatattc tttgttttag agatagatac ttatagcttg tggattaaaa
     8881 ataatataaa tagaagatag attttttaga tatattgagt tgaagattat atagaatgtt
     8941 gaaacatagc tatttaaatt attgtctata aatataaaat gaatggtggt gagttaagtg
     9001 accgatttag ttaacaccat taatggaatt gtttggtcac ctgtattagt tgcaatgtgt
     9061 ttgtgtatag gattatattt ttcaatgaaa ttaagatttt ttcaaataaa agatgtaaaa
     9121 gaaatgttta ctcttttgct tgaaggagat agctctcatg ttggaatatc ttcgttccaa
     9181 ggttttgcta catctatggc aggaaggata ggaactggaa atatagctgg tgtagctgta
     9241 gcgatagcaa tgggagggcc aggagcatta gtgtggatgt gcataatggc tacattaggg
     9301 tcggcaactg catttgtaga atctacttta gctcagatat ataaagatga gcatgatgga
     9361 cagtacaggg gaggtcctcc atattacttt gaaaaaggtc ttggttggaa aaaatgttca
     9421 attttatttt cagtggttgc aatcataagt tatacatttt ttatgcctgg cacacaatca
     9481 aatactctat atctagcaat gaatcaagca tttggatttt caaaaatgac aatagcagtt
     9541 acatcttcta ttgcactagg tataataatt tttggaggaa ttaaaagaat tggtttattt
     9601 gctgagaaag tagtacctat aatgggtgga gcatacctta ttatgacatt ggtaataatc
     9661 atagcaaata taaatctagt tccaggagct attatgacaa tgtttaaatc tgcatttgga
     9721 tttggttcgg tttttggagg gatgttaggt tcagcaatat cttggggagt aaaaagaggt
     9781 atatactcaa atgaagcggg agaaggttct ggaacttatg gtgcagcagc agcagaagtg
     9841 agtcacccag ccaaacaagg attagtccaa gctttttctg tttatgtaga cacactatta
     9901 atatgtatgg ctacaggtat tatgattata ataacaggaa tgtataatgt tacagatgtt
     9961 tacacaacat taccaggtgt agaaccaggt ccacaatatg tacagagtgc aataaataca
    10021 ttacatcctc aattaggtga tatttttata gcaatagttt tactactatt tacatttaca
    10081 acattattag ccttctcata catgatggag accaacgtat cttacttagt gactagaatt
    10141 aaggcagaaa aacatgaaaa gcacataatt aatgtctgta gatttatgct tataatatgt
    10201 gcagcaatgt gttgctttat agaaccactt acaagttggg cacttggtga tatatgtata
    10261 ggtgtattaa catatataaa cttagttggt atatttacac taaaaaaacc agctataaaa
    10321 gcatattacg attatcaaag gcagaaaaag atgggagtaa aaaggagcga tagaactttt
    10381 aatcctatag aattagatat aagaaatgct gagttttggg aaaatagata cttaaaagag
    10441 gataagcatg agttaaatta aagttataat aaaatataaa aattactaaa taaatttagt
    10501 gggaggtaag attggtatga aagaaagaat aatacaagtg gctgattcaa agaaagaaaa
    10561 aattctaggg ctatgtcaat ctttatatga tgaaccagaa attgctttgc aagaatataa
    10621 atctgctaaa aaaatatcag aatttctaag agaagagggt tttgatgttg aggaaaactt
    10681 ggcaggtatg gctacagctt ttaaagcaac caagaaaaat ggagatggtc ctaaaatagc
    10741 atttatagca gaatatgatg ctcttccagg caatggacat gcatgtggac atcatttaat
    10801 tgcatctatg ggagttggag ctggaatagc tctttcaagt atactggata cttacaaggg
    10861 agaagtaagt ataattggaa cacctgctga agaaactgga gatggaaagc cttacttgat
    10921 agaacatggt gtatttgatg gctatgatgc agcaatgatg atacatccaa attcaaaaac
    10981 ttgtgtaaca cctgaaatta tagcaatagg aggtctagat ttcatattta ctggaaaagc
    11041 atctcatgca ggagctaagc catataacgg aataaatgct ttagatgcag tagtactact
    11101 atataataat ataaatgcac taagacaaca attagtagat ggaacgagaa tacatggtat
    11161 tatactagaa gcaggaactg ctgcaaatgt aatacctgat atgggaaaag taagattaga
    11221 gataagggca aaagaacaaa attactttga tgaagtagta gaaaaagtga aaaattgtgc
    11281 tagaggtgct gcaatagcca ctggatgtga acttgaattt tatcactttg aaccaacttg
    11341 ccaaggatta aatgaaaata aagttttggt agacatattt acaaagatta tggaggaatt
    11401 tggaatatat gaagatgaac aagtaatgtt aggttcaaca gatatgggaa atttaagtca
    11461 aataatgcca tgtatacatc ctttgatgaa gttttctgaa aatggagaag aactacatac
    11521 aaaagaattt ttggaggctt ctataaattc atatgcaaaa gatagagtaa ttgatggtat
    11581 aaaaatatta gctttaacag gatttaattt atttgaaaat ccagagttat tgaaaaaaat
    11641 gaaggaagag tagaacttag actattttta attatactct ttaaaataag aagagtgtct
    11701 caaaatgaac tttttagttt atgagacact cttttttgca tgaaattaaa tatattttta
    11761 ttattttaac aatgaaaaag aggcttatct gtttttatat aacctttttt aacttgtagc
    11821 acaaaatatc atgaatgatt acaattcgaa tgataaaatc gattataaga tatataggag
    11881 gggtacatat ggaaacttta gaagtaagaa atattgaatt gagtggtaca aattatgaga
    11941 taggatacag actaggagaa ttggttgcta atatgccaga gataatagaa ggtcagatta
    12001 ataaatctaa tctggtaagc aaaaaagaag aaaaagagat gatagagttg tttgataaat
    12061 attgtccagg tcttaatgaa gaattgcaag gttttgctga tgcaattcaa gtgaattgta
    12121 atcaaatttt atattatact atgacttatc ttaaaccagg ttgtagtcaa gttgcattaa
    12181 caccagaatt aactgaaaat gggcatgtac tatttgcacg taatttcgat ttctcacaca
    12241 atatggagga ttttgtttta tgtaaaacta aagtaaatgg aaaatatgca catataggta
    12301 ctaccgttat gcaatttgga agaggtgaag gtatgaatga atgtggatta ggtgtaagtc
    12361 agtcatcatg tggtattcct gtaggcaatt cagatggttt gagaaaacca gcaattgtag
    12421 gtttacagtt ttgggctgtt attagatatt tgttagaaaa ttgcaaagat gtagatgaag
    12481 cattagaata tttaaaagat atgccaattg catacaatat taatttattg cttgcagata
    12541 agtctggaaa cattgcactt gtggagactt tagatgggaa aaaagaagta aatataatca
    12601 acagtttaga aagtaagaga gaatattttt tacactctac taatcataca catattgata
    12661 aacttcataa attagaacca caatcaatga aaaattctat tcatagatat aaattaatta
    12721 aagaatatat aaataaggct aaaaaaattg gagagaaaga gttaatgaat ttattgtctt
    12781 taaagtatcc aaatggctta tcttgtaatt actataatga tttttttgga actttaaaaa
    12841 gtatagttat ggatttaaat ataggaaagt ttaatatttt atggggtgga ctagagaata
    12901 aatgggagag ctactattta aaaaatgaca ttaaatctat aacacagagg ataaacataa
    12961 atattgaaaa agcaccatct gacttttttg atttcattga ataaaaaatg tatttaatta
    13021 ttctaaataa ttaggctata taaatcaact aaaagttttt atttatcgta ttgaattagt
    13081 tataatattt tagtatagta aaaaatagtg ctattttaca tatttaaaga gtttaagtag
    13141 aagagaggtg ccatattgga ttacttaaag tattttgaaa aagctgtaat gtatattgag
    13201 aataatttac atgaaaatat aactgtaaat gatgtggcaa aagaaactgg atattcttat
    13261 tatcatttaa cacgcttatt taagtctatg tttggtgaga gtgtaggaag ttatataaaa
    13321 aaaagacgat tagtaagtag tacaaaagaa cttttatata gtgataaaaa agtgatagat
    13381 atagctattg gtagtggatt tgaatcttcg gaagcattta gcagagcatt taaatctata
    13441 tacaaggtta gccctataga atatagaaaa aatagaattg atgtatttgt tggtaaaaag
    13501 aaaaaattag agttagattt tatgagacat cttgttggaa atataacgat taaaccagtt
    13561 ataaaagaaa tagaaaaaat aaaggtaata ggtataaagg ataaagtaat tttagaaaat
    13621 aattcattac ctgacttgtg ggagaaattc agaaaagtac atcatattgt cccaaatact
    13681 ttgccatcaa agagagtttt tggaatctgt gaagcaatct ctgaaataca cctagttagt
    13741 gaaagtatgg aatttaatga gataatagga cttgaagtta atagttatga tgtgattcca
    13801 aattcatttg tttcaaaaac tattaaggaa ggaaagtatg ctatatttac tcatactggt
    13861 agcttagata acttagacaa aacttatgaa tatatatggg gtacttggtt tttaagttca
    13921 aaagaagaac tggatataag agatgatttt gaagtatatg atgaacgatt tttaggtcct
    13981 agtaatgtga actcacaaat tgatatttat atacctataa aataatctat aagaaaacta
    14041 aaagataatt tgattaagaa atagatgttc gtaaaagagc atctattttt tatttttatc
    14101 agatttagat aattttaata ataaaagaaa taaaaatata ttataaaaac aatttgattg
    14161 gaattttcag aaaattaatg ctataattaa aagtaaattt aaagggaggt tataaaatgg
    14221 ctaaattaac tctagaacat ataaaaggaa atacatatta tattccactc cctacaatag
    14281 taggtgtata cgtagatggt aaagatgcaa ttttaattga cagtggaaat aataaggata
    14341 cagctagaca agttttaaga ttattagaag aacataattt aagtcctaaa ttaataataa
    14401 acactcattc aaatgcagac catataggag gaaatgcata tttgaagaat caaacaaaat
    14461 gcaagattgc tacgactaaa attgaaggat attttacaga aaatccaata ttagaatcag
    14521 catttttata tggtggctat ccaagtaaag cactgaagaa taaattttta ttagcaaagg
    14581 aaagtgaagt tgattatatt ataccttcaa atggcaaaat tgtagataca gaacttgaag
    14641 cgatttctct tccaggtcat tattttgaaa tgattggtgt tagaactcca gataatgttt
    14701 tgtttgttgc agatagtctt acaccagaga atataattac taaatatcat tttttcttcc
    14761 tactggatat agattcacag tttaagacac tagaaaaatt gagaatgtta gaggctgatt
    14821 tctttgttcc aagtcactct gtcaaaacta cagatataaa aagtttgatt aatattaata
    14881 agaagaagat ggaagaaata atagataata ttaaaaaggt ttgttatgag ccagttatga
    14941 ttgatgaagt tattgaaaaa atgtgtgatt tgtacaatgt caagcttgat gctaatcaat
    15001 atgtgttggt tggaagtaca atacgttctt atataactta tttatatgag aataatatgg
    15061 ttgaatatat atttgatggt ggtaagatga tgataaaagc tgtgtaaata gtaataattt
    15121 tcaaattata tgatgaaatt gttttagtat gactgttatt tgaaaagtaa cataaaaaaa
    15181 tttatataaa taagctaaga tgattgatat aaagttactg ataattttaa ggtaatatat
    15241 acgttactat aatattatgt tattatttgt aaataagggg ataatttata agcaaattaa
    15301 agaccttaat ttacaggagg aatagtgtat attatgaata aaaaattcaa aaaattcagt
    15361 aaaaaactaa gtatattaat tttagttttg actatgtttc tttttaataa tttaactgca
    15421 aatgcacaga ttaaagaaga ttcaataata ggaattgata gatatgaaac agcaggactt
    15481 atagcagata gacaaactta tgatactgta atattagtta atggagataa aaatttatct
    15541 gatgggcttt catcaagtgg attagctgga gctattaacg caccaatact tttgactaaa
    15601 aaaagtgaaa taccaaaagc tactttaaat agactagata ataagagttt aaatgttgta
    15661 aaaaaagtat atataattgg tggttataat actattgaaa attcagtaga aaaagatata
    15721 aaaagaaaag gtattcaagt ggaaagaata aatggtaaca atagaattga aactagttat
    15781 aatgtagcta aaaaaataaa tgaagtaagt agtgtaaaag aggtaatgct tacaaatgga
    15841 tttgtaggag aggctgatgc tatgagtata gcacctgtag ttgcaaaaaa taaaggagct
    15901 ataatactta cagatgggaa atcagttcca tttaagactg aaggtttaaa tgtttacgct
    15961 ataggtggca aatctgcgat aaatgaggat ttagtaaatg aaactaaagc aactagaatc
    16021 ggaggaaatg atagatttga gaccaataaa aaggtaatgg aaaagtttta taatggagcg
    16081 acagattttt atataacaaa tggatatcaa ttagtagatg cactaacatt atctccttta
    16141 gccaaagaaa aaccaatcgt tttagttgct gatggaagta ataaaggtat tttaaaagga
    16201 gctaaaagta taacaaaagt tggaggtatt gatgccaata tatataaaca gtgtttagat
    16261 atgattaaat ataataatat ggatgtgact ccaaatattt taaaacactt aacttcagtt
    16321 gaaggaaatg aagtttctga aatacttata gaatcagata atttaggtgt ggttgttgaa
    16381 aagacgaata ccaataaatt tgagtttgat tacgtaagtg taactaatga aaaaaattgt
    16441 actttttctg tatataaaga aaattcttct aataatgtaa aaaatggaaa gctagttgta
    16501 agtgctaaga aaaaaataga taagaaagat agtgatttgc aagatatgaa tggcgacaat
    16561 atgataaatg caaataaaga taggatggta aatgtgataa aaattggagt tcccgataaa
    16621 atgtattcta attttaaagt agatgttgaa agtggttcag tagaacttta taatattaaa
    16681 ggaggagcta ctgttgatgt caatgacgga attgctaaca ttgttgataa caatgtgaca
    16741 aatcctttca atataaatac taatagtggg atttcaggtg taacagctga aactatatca
    16801 tctgagataa aatttagagc aaacaatgga attgtaagtg taacagctac aaatataaat
    16861 ggcaatataa gtttatttgg aagcgataat aaaggtacct ttgatggaat atttaaatta
    16921 aatctgaaaa aagaaccatc aaatttacac ttgaagttag ttggaaatgg gataaataaa
    16981 ttgccagaag gttggtctaa agattatatt ttaggaaatg gacaaccaat tattgaagtt
    17041 aaaaataatg gcataaataa tatatcaatt ggagaataaa aac
//
LOCUS       DAMJWP010000062        17041 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.064, whole genome shotgun sequence.
ACCESSION   DAMJWP010000062 DAMJWP010000000
VERSION     DAMJWP010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17041
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.064"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(74..760)
                     /locus_tag="PC700_001735"
     CDS             complement(74..760)
                     /locus_tag="PC700_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDF2581047.1"
                     /translation="MFGVIANSLAIAGGCIVGLIIKGGLPQRVSDTIMNGIALCVLYI
                     GISGALEGKNTLVTIISVAVGALIGELIDIDKWVNKLGAFLQSKFSKGNKKDSIAEGF
                     ISSSLLFCIGAMAVVGSLESGLTGSHDTLFVKSVIDGIASIIFTASLGIGVMFSAISV
                     FLYEGIICVGASFLNGFLSDPVVTEMTAAGSLLIIGLGLNVLKLTNIKVANLLPAIFI
                     PILFGVFGII"
     gene            complement(809..1342)
                     /locus_tag="PC700_001736"
     CDS             complement(809..1342)
                     /locus_tag="PC700_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDF2581048.1"
                     /translation="MSDLNNKKEQDENESSMNETKIYDILDKLNVEYDVVEHEAVYTA
                     EQLVILDGITKGCQCKNLFLRNAKGNKYYLIVVKGEKQVDLNSLKDKIGSSRLSFASP
                     ERLYNVLKLLPGSVNPFSLINDTERKVELYIDKDVIKEDYLNFHPNINTKTVNISRKG
                     FEEFLEYLKYPIQSIDA"
     gene            complement(1386..2018)
                     /locus_tag="PC700_001737"
     CDS             complement(1386..2018)
                     /locus_tag="PC700_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HDF2581049.1"
                     /translation="MKVFDLTHVTHNDMPVYAEPNRPDIKKVAIIEENGYQETLISVF
                     SHNGTHMDSPRHMYTKGETLDKLDIENFVGKAYVLELEKGNENIELEYLKKYEDEIKN
                     SDFIIFKSGWSKFWDKKQYYVGYPTLTKEAANYIANTNIKGIGIDMLSVDRYNTSVFE
                     VHHILFEKGKIIIENLTNLENVPEKFLFIAAPFKYNDADGAPVRAIAIVE"
     gene            complement(2108..3187)
                     /locus_tag="PC700_001738"
     CDS             complement(2108..3187)
                     /locus_tag="PC700_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HDF2581050.1"
                     /translation="MKIAIFLGAGASAAENLPIQNELFSEYFKKLNPTNYNTKMNREL
                     YIFFKQMFNIDIVKDNIDNANFPTFEEVLGLLDLAEQRKEAFRNFGLENLNNRSDSIR
                     FLRQYLILLMAEAIHNTPRTDNKYHKLLVENLLENDLLLDTTFISANYDIHIDNTVTG
                     LRDKNDLPIMLDYGVEFTNFKEELNWRKPKEPLIKLYKIHGSLNWLYCPICNSVTLTP
                     HEGGVMKLIENSSETKCLECGELTEPIIVPPTYFKNMSNIFLSNVWNETEKTLRDTDL
                     LIFCGYSFPEADMHIKYMLKRVQTNRKKPPLKIMVFNNHSQKQRITLKKEEGRYKRFL
                     GEDVIFTDNSFQDFSVNPLRFIKNI"
     gene            complement(3320..4666)
                     /gene="pepV"
                     /locus_tag="PC700_001739"
     CDS             complement(3320..4666)
                     /gene="pepV"
                     /locus_tag="PC700_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HDF2581051.1"
                     /translation="MKEQIKEKVNSLQDEMISSIQESVKIPSVISEATENCPFGENVD
                     KALRGILDLCKSLGFKTVYKDGYYGYAEIGQGEKMIGILGHVDVVPEGDLESWNYPPF
                     EAVLEDGKLYGRGTQDDKGPTISAIYAVKALMDLNVDFNKRIRFIFGADEENLWRCIN
                     KYKENNEEIPNYGFTPDSRFPITNAEKGLLQVHLTCDSKSDIELSVGKALNAVPGKAI
                     YIGKYSDKLKKELDKLNFEYTVEGNKICIIGKSVHSAASDTGINAVARLCIALNNIGI
                     DSNIIKFLAEVIGEDANGNNIIPNCKDDVSGKLTVNIGRVTIDNEKEFAGIDVRIPVT
                     YKKDDFVKELKKMTDKYNLNYEEYDFLDSIYVPEDTLLVKTLRKVYEEETGLDGTPLS
                     SGGATYARALDNCVAFGAIFPGKPETEHQANEYLIVEDIIKATQIYALSIYELLKI"
     gene            complement(4735..6015)
                     /locus_tag="PC700_001740"
     CDS             complement(4735..6015)
                     /locus_tag="PC700_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HDF2581052.1"
                     /translation="MLNETKELLKDYYGIDDDTFKLSQEIMEEIKDKFEEIKEIREYN
                     QYKVLKAMQESKLSDMHFNWTTGYGYNDIGREKIEEIYSKVFNTEDALVRPIIVNGTH
                     ALTLCIQGIVRPGDEILSVTGRPYDTLEGVIGIREEKGSLKEYGVTYDDVDFLEDGNL
                     DLEGIKNKINDRTKLVMIQRSKGYSWRKSLSISDIKEAIEVIKSVKPEAIVMVDNCYG
                     EFLDTKEPTDVGADVMAGSLIKNPGGGLALTGGYIAGRKDLIELISYRMTSPGIGKEC
                     GLTFGTTRNVLQGFFLAPYIVSQAVMGAIFCSRAFEKLGYDVLPKYDDLRSDIIQCIR
                     LNNADEVISFCEGIQEAAPVDSYVKPVPWDMPGYESEVIMAAGAFIQGSSIELSADAP
                     IRPPYNVYFQGGLTFDHSKMGTLKAIEFIKKLKK"
     gene            complement(6142..6402)
                     /gene="hfq"
                     /locus_tag="PC700_001741"
     CDS             complement(6142..6402)
                     /gene="hfq"
                     /locus_tag="PC700_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HDF2581053.1"
                     /translation="MKNTVLNLQDLFLNNARKERIPVTIYLVNGVQVKGLVKGFDSYI
                     ILIEGDNRQQNMIYKHAVSTIQPGKYINLTNQNQNNNNNNNR"
     gene            complement(6516..7451)
                     /gene="miaA"
                     /locus_tag="PC700_001742"
     CDS             complement(6516..7451)
                     /gene="miaA"
                     /locus_tag="PC700_001742"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDF2581054.1"
                     /translation="MKKIPLIILTGPTAVGKTDLSIKLAKDIDAEIISADSMQIYEYM
                     DIGSAKVTEEEMQGVKHYLIDEVKPDYPFSVSEFQQRAKKYIHEINEKGKCVLVTGGT
                     GLYLNSLIYNMDFAQSDANNELREELQKQLAEKGIDYMHNKLKELDEESANRIHKNNT
                     KRVIRALEVCLSGKKMNDFSSDLKFNEEYQPIIIVLNRDREHLYQRINMRVDIMIKNG
                     LVEEVKKLLSMGFKKDMISMQGIGYKEILKYLDGEYTYEEAIEIIKRDSRRYAKRQIT
                     WFKRYKTARWFDLDQYENIDELKNEIILYIKDSIK"
     gene            complement(7489..9456)
                     /gene="mutL"
                     /locus_tag="PC700_001743"
     CDS             complement(7489..9456)
                     /gene="mutL"
                     /locus_tag="PC700_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDF2581055.1"
                     /translation="MKNIINILDDLTINKIAAGEVVERPSSVVKELIENSIDAGANKI
                     SIDIIDGGKSLIKITDNGIGIPSSEVEKSFLRHATSKIKKIDDLYDLYSLGFRGEALA
                     SISAVSKLEMTTKTKDEIIGTKIYVEGGKIISKEPIGSTNGTTIIIKDIFFNTPARQK
                     FLKSTHAETINISDLINKLAIGNPNIQFKYTNNNKQMLNTPGDGKLVNTIRSIYGKEI
                     TENIIDVEFKCNHFKMNGYIGNNNIYRSNKNLQHIYINKRFVKSKIIIDAITESYKSI
                     IPIGKHAVCFLNIEVDPSCIDVNIHPNKLEIKFEKEQEVYIELRDFLKVKLIHSNLIG
                     KYATYSDKKTQPRIAINSREKSTDYKLRNNDLLESTPKNSNITKDKDEVIEVVTLSSE
                     KPINEFQSVSEVLNASVEDDVKNINYLSEDSANDNIQEEFQVDGIKNEGNYYLGDSIK
                     DSEEEYLCSSKRKFSLYGYSVIGVVFNTYIILSKDDSMYLLDQHAAHERILYERYMEK
                     FYRQDINMQILLDPVVIEVSNVDMLQIENNLELFMKFGFELEIFGNNHIMVRCVPTIF
                     GVPETEKFILQIIDNIEEITSNYDLKGERFASMACRSAIKANDKIYDIEIKSLLEQLE
                     KCENPFTCPHGRPIMVEISKTEIEKMFKRIM"
     gene            complement(9467..12310)
                     /gene="mutS"
                     /locus_tag="PC700_001744"
     CDS             complement(9467..12310)
                     /gene="mutS"
                     /locus_tag="PC700_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDF2581056.1"
                     /translation="MQIDMNKLTPMMKQYLEVKNRYKDCILFFRLGDFYEMFFEDALV
                     ASKALEIALTGKACGLEERAPMCGVPFHSANSYISKLVENGYKVAIGEQMEDPSTAKG
                     IVRREVIRVITPGTVLDGNLLENKKNNYLLSLYKDGTNIGLTYVDISTGETNATCLNE
                     DKVIEEIAKIHPTEIIINDLDFIEKLRDIATVSNIYINESFSDNYLDINILKEYFPDV
                     YLQKLKFDDKGLIKSSLSILLNYIYNTQKQITSNINNINIYNSSEYMVLDMFTRTNLE
                     LTQTIRGNKKKGSLLHVLDKTSTAMGGRLLRKYVEEPLINKSKIENRLDVIEEIKDDF
                     ILREDLNDILKNIYDIERICGKIAFERVTPKELIHLKNSIEKLPNLKDTINLSNAKIL
                     KEYVSEMDKLDDIYNLIDEAILEEPTITIKDGNIIKSDFSDELKELREISKNGAFLVK
                     EIENREREKTGVKSLKIGFNKVFGYYIEITKANFKQAKLDETYIRKQTLSNAERYITP
                     ELKEIEEKILHAEEKIKSLEYEIFVEIRDTIYKNIDRIQKVAKTIANIDVFVSLATVA
                     HINNYVKPAINENNKLDIRNGRHPVVENIVGEENFVPNDTYLNRGENIINIITGPNMS
                     GKSTYMRQTAIIALMAHIGSFVPAESADIPILDRIFTRVGASDDLSQGQSTFMVEMNE
                     VSLILKNATERSLVILDEIGRGTSTYDGISLAWSIVEYIQKNIRCKTLFATHYHELTD
                     LEEEFKEVKNYSIAVKEDGEGIIFLRKIIPQGADKSYGIYVAKLAKLPDEVIERAKYI
                     LKDLEKNHVYNSVAINGDKENNNIINSNLDEELVKVNQSNFKNKYESLKIEHELIVKD
                     YKHIKKDYDKLNSKFKALNDEVALMKQNDDKEKINQEDSVKEVALTQISFDSVNRDIL
                     SEEILNLDILNMTPLDAINSLYNLQRKAKEIKS"
     gene            complement(12473..13207)
                     /locus_tag="PC700_001745"
     CDS             complement(12473..13207)
                     /locus_tag="PC700_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2581057.1"
                     /translation="MLTQMNFYHNKKVKVSIENVSKTYKDIEVLKDISIDVYEGEFVS
                     ILGPSGCGKSTIFNIITKLTDYDSGKVNINGKYSYMYQKDLLLPYKTIIDNVSLPLIL
                     KKEKKSKARSMVKPYFEVFGLSGYEDKYPSELSGGMRQRANFLRTFINSNDIMLLDEP
                     FGALDSLTKTSMQKWLLDVKKKVNSTILLITHDIDEAIMLSNRIYVISKKPSIVKKEF
                     VIDSRKLNEDNLENIIKLKKEIISLL"
     gene            complement(13176..14189)
                     /locus_tag="PC700_001746"
     CDS             complement(13176..14189)
                     /locus_tag="PC700_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2581058.1"
                     /translation="MNFKKLGILGLVSVFTLATITGCSSNNNKDKNNDKDTSSKKVTM
                     VLDWTPNTNHTGLFVALDKGYYKEEGLDVEIVQPPESGAETLVATGKADFGISYQEQV
                     TYAKTSEDPLPIKAVATVIQHNTSGFASPKEKNITTAKDFEGKTYGGWGSPSEEAVFK
                     AVMKKNGADFNKLKIVNTGQDDFFAAMKTVDFAWIFEGWDAVKADLIGYDLNFIPVKD
                     LDERLDYYTPLIISNETVLKDNPELAKKFLKATTKGYEYAIKNPEESAKILVKHAPEV
                     DEKLALKSQEYLASKYKDDAPRWGEMKDSVWNNYTSFLKEYKLIDKDMKASDAYTNEF
                     LPQ"
     gene            complement(14211..14981)
                     /locus_tag="PC700_001747"
     CDS             complement(14211..14981)
                     /locus_tag="PC700_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2581059.1"
                     /translation="MKNLEIIDKVKDSIYPIATFLFILILWQSMVGVLEVPQYILPTP
                     VDIINVFFKDYQNLSMHAVVTIGEAILGFIVAIIISLVIGILMDFVSIIKKCLYPIML
                     VTQMIPTIIIAPLFMIWFGFGTMPKVLMVMLTCFFPILISFVDGIENIDKDYLNLFKT
                     MDSNKVNTFIHLKFPMAMDKFFSGLKISATYAVMAATVAEWLGGTKGLGVYMLRSKSA
                     YALDKVFASTILVVIFSLMFVGIVQAVKKVVIRHRLID"
     gene            complement(14956..15246)
                     /locus_tag="PC700_001748"
     CDS             complement(14956..15246)
                     /locus_tag="PC700_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HDF2581060.1"
                     /translation="MVIADIAVMPLRPYKGEEEMYKVVDACIAEIEKSGLKCEIGAMS
                     TTVEGDFDEVFELLKKVHKIPFNLGCERVITVARVDEKAGGLTIDEKLRNHR"
     regulatory      complement(15377..15476)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     regulatory      16096..16185
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            16238..16414
                     /locus_tag="PC700_001749"
     CDS             16238..16414
                     /locus_tag="PC700_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581061.1"
                     /translation="MFMDKMDRCTHILTAYICSSRDYCNFIDTQLNDFILEYGENVVE
                     SCLHQVMVLVSRYN"
     gene            complement(16529..16885)
                     /locus_tag="PC700_001750"
     CDS             complement(16529..16885)
                     /locus_tag="PC700_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2581062.1"
                     /translation="MDIDNNFEEFPNIEERQKDFNCSIGFTMTVIGSKWRAIILWHIL
                     KQEPIRYSQLKKDVVHISHKILSQELKYLERDGLIKRISYPTIPPKVEYLSTKRGKSL
                     ESILLELCNWGKQYMR"
BASE COUNT         5377 a         2925 c         1797 g         6942 t
ORIGIN      
        1 tctaaataaa aaatttgtct aaagtttcta aaaaatttag acaaatctta ggttattatt
       61 ctttaaatta tttttaaatt attccaaata caccaaataa tattggtata aatatagctg
      121 gtaacaaatt agctactttt atattcgtta atttaagtac atttagacca agtcctataa
      181 ttaataaact tccagctgct gtcatttctg tgactacagg atcacttaaa aatccattta
      241 aaaatgatgc tcccacacat ataatacctt catacaaaaa tacagatatt gcagaaaaca
      301 ttacacctat acctaaagaa gcagtaaata taattgaagc tattccatct ataacagatt
      361 ttacaaataa agtatcatga cttcctgtaa gaccactttc taatgaacca actactgcca
      421 tggctcctat acagaataat aaactagatg atataaaacc ctctgctata gaatcttttt
      481 tatttccttt agaaaattta ctttgtaaaa aagcacccaa cttatttacc catttgtcaa
      541 tatctattaa ttctcctatc agagcaccta cagctacgga tattattgtc actaatgtgt
      601 tttttccttc tagtgctcct gatataccaa tgtacaatac acataaggca attccattca
      661 ttatagtatc acttactctt tgaggtaatc ctccttttat tataagacct actatacatc
      721 cacctgcaat tgctagacta tttgctatta ctccaaacat tttttctccc ctcgattagc
      781 taattgaaat ttatcctatt attaaaacct aagcatctat actttgtatt ggatatttta
      841 aatattccaa aaattcttca aaaccttttc tagatatatt tacagtttta gtatttatgt
      901 taggatgaaa atttaagtaa tcttctttaa ttacatcttt atctatgtaa agctcaactt
      961 ttctttctgt atcatttatt aatgaaaaag gatttacact tccaggaaga agtttaagta
     1021 cattgtatag tctttcaggt gaagcaaacg ataatctaga acttcctatc ttatctttaa
     1081 gtgagtttaa atcaacttgt ttttctcctt taactacaat aagataatac ttatttcctt
     1141 tggcatttct taaaaataaa tttttacatt gacacccctt tgttattcca tctaaaatga
     1201 ctaattgctc tgctgtatat acagcttcat gctctactac atcatattca acatttaatt
     1261 tatccaaaat atcatatatt ttagtttcat tcatgctaga ttcattttca tcttgttctt
     1321 ttttattatt taaatcactc atacttttct ccttaaacac ttcatcataa tatcattgtg
     1381 aggtattatt ctactatagc tattgctctt actggagctc catcagcatc attatactta
     1441 aatggtgctg ctataaatag aaatttttct ggaacatttt ctaaatttgt aagattttct
     1501 atgattattt ttcctttttc aaataaaata tgatgcactt caaatactga tgtgttatat
     1561 ctatccactg atagcatatc tattcctatg ccttttatat tagtattagc tatatagttt
     1621 gcagcttctt ttgttaatgt aggataacct acatagtact gctttttatc ccaaaatttt
     1681 gaccaacctg atttaaatat tataaaatca ctatttttta tttcatcttc atattttttt
     1741 agatattcta attctatatt ctcatttccc ttttcaagtt ccaatacata agctttccca
     1801 acaaaatttt ctatatccaa cttatctagg gtctcacctt tagtatacat atgtcttggt
     1861 gaatccatat gagttccatt gtgagaaaat acactaatta atgtttcttg ataaccattt
     1921 tcttcaataa tagctacttt cttgatatct ggtctattag gctctgcata tacaggcatg
     1981 tcattatgtg tcacatgagt taaatcaaaa accttcatct ttatataccc cctatgtttt
     2041 tattttctat tatataccta aattcatttt atttctatca gattttattc cattatatac
     2101 catactacta tatatttttt ataaacctca agggatttac cgaaaaatct tggaaagaat
     2161 tatcagtaaa tataacatct tcacccaaaa aacgtttata ccttccttct tctttcttta
     2221 aagtaattct ctgtttttgt gaatgattat taaaaaccat tatcttaaga ggaggttttt
     2281 ttctattagt ctgcactctt tttaacatgt attttatgtg catatcagct tcaggaaatg
     2341 aatatccaca aaatattaaa aggtcagtat ctcttaaagt tttttctgtt tcattccaaa
     2401 cattacttaa aaatatgttt gacatattct taaaatatgt tggtggcact ataataggtt
     2461 ctgttaactc accacactct aaacattttg tctctgaact attttcaata agcttcatga
     2521 caccaccttc atgtggtgta agagttacac tattacaaat ggggcaataa agccaattca
     2581 atgacccatg tatcttatat aatttgatta atggttcttt tggttttctc caatttaact
     2641 cctctttaaa atttgtaaac tccacaccat aatcaagcat aattggcaaa tcattcttat
     2701 ctcttaatcc agtaactgta ttgtctatat gtatatcata atttgcacta ataaaagtag
     2761 tatcaagaag caaatcattt tccaaaagat tttcaactaa aagtttatga tatttattat
     2821 ctgttctagg tgtattatga attgcttcag ccattagcag tattaaatat tgtcttaaaa
     2881 aacgtatact atcacttctg ttatttagat tttctagtcc aaaatttcta aatgcttctt
     2941 ttctttgttc tgctaagtct aaaagaccta aaacttcttc aaaggtagga aaatttgcat
     3001 tatctatatt atcttttact atatcaatat tgaacatctg tttaaagaaa atatacaatt
     3061 ctctattcat tttggtatta taatttgtgg gatttagttt tttaaaatat tcgctaaaaa
     3121 gttcattttg aataggtaga ttctctgctg ctgaagctcc tgctcctaaa aatattgcta
     3181 ttttcatata gttcctccta atataaatat tactcctatt tttatatcca ttaaatatcc
     3241 atatcattta ttttatgata caacaataaa gctgtctttt actagaaaaa atcctattac
     3301 aagacagcct tatattatat taaatcttta ataattcgta aatagaaaga gcatatattt
     3361 gagtagcttt tataatatcc tctactatta agtattcatt agcttggtgt tctgtttctg
     3421 gttttcctgg aaaaatagct ccaaatgcaa cacaattatc taaagctctt gcgtatgttg
     3481 ctcctccaga tgaaagtggt gttccatcaa gaccagtttc ttcttcatat acttttctaa
     3541 gtgtcttaac aagaagagta tcttctggaa catagattga atccaagaaa tcatattctt
     3601 cataatttaa attgtattta tctgtcattt tctttaactc ttttacaaaa tcatcctttt
     3661 tgtatgttac tggaatcctt acatctattc ctgcaaattc tttttcgtta tcaatagtta
     3721 ctctacctat atttactgtt agtttacctg atacatcatc cttgcaatta ggaattatat
     3781 tgtttccatt agcatcttca ccaataactt ctgctaaaaa cttaattata ttagaatcta
     3841 tacctatatt attaagagct atacaaagtc tagccactgc atttatacca gtatcacttg
     3901 ctgctgaatg gacacttttt ccaattatac agattttatt accttcaaca gtatactcaa
     3961 aatttaattt atcaagttct ttttttaatt tatcagagta ttttccaata tagattgctt
     4021 tacctggaac tgcatttaaa gcctttccaa ctgacaactc tatatcactt ttgctatcac
     4081 aagtcaaatg aacttgtaaa agacctttct ctgcatttgt tattgggaat ctagaatctg
     4141 gagtaaatcc atagtttggt atttcttcat tattttcttt atatttattt atacatctcc
     4201 aaagattttc ttcatcagcc ccaaaaatga atcttatcct cttattaaag tctacattta
     4261 aatccataag agctttcact gcataaattg ctgatattgt aggtccttta tcatcttgtg
     4321 ttcctcttcc atacaattta ccatcttcta aaacagcttc aaatggaggg taattccagc
     4381 tttctaaatc accttctgga accacatcaa cgtgaccaag tatacctatc attttttcac
     4441 cttgtcctat ttctgcatat ccataatatc catctttgta tactgtctta aatcctaaag
     4501 atttacataa gtctaatata cctcttaaag ctttatctac attttcacca aatgggcaat
     4561 tttcagttgc ttcagatata acactaggta tttttacact ttcttgtatt gaagaaatca
     4621 tttcatcttg tagagaattt actttttctt taatttgctc tttcatatta tcacccttca
     4681 attttcatca taatatacta ataaatattt tgaccttttt aaaaaaaaat atgcttattt
     4741 tttcagtttt tttataaatt caatcgcttt taaagtcccc atttttgaat ggtcaaatgt
     4801 aagtccacct tgaaaataaa cattataagg aggtcttatt ggagcatctg cacttaattc
     4861 tatagaagaa ccttgtataa atgctcctgc tgccattatg acttcacttt cgtatccagg
     4921 catgtcccaa ggaacaggtt ttacataaga atcgactggt gcagcttctt gtataccttc
     4981 acaaaaactt attacttcat cagcattatt aagtctaata cattgaataa tatcacttct
     5041 taagtcatca tattttggta atacatcata tcctaatttt tcaaaagctc ttgagcaaaa
     5101 gatagctccc attacagcct gagatactat ataaggcgcc aagaaaaaac cctgaagtac
     5161 atttctagtt gttccaaaag taagaccaca ttcttttcct attccaggag aagtcattct
     5221 ataagatata agctcaatca aatcttttct accagctata tatccacctg tcaatgcaag
     5281 cccaccacca ggatttttta ttaaagagcc agccataaca tctgctccaa catcagtagg
     5341 ctctttggta tctaaaaact caccataaca attatcaacc attactatag cttctggttt
     5401 aacagacttt attacttcta tagcttcttt tatatcacta attgaaagtg attttctcca
     5461 agaatatcct tttgaccttt gtatcataac gagtttagtt ctatcattta ttttgttttt
     5521 aattccttct aaatctaagt ttccatcctc taaaaaatct acatcatcat atgtaacacc
     5581 atactctttc aatgaacctt tttcttctct tatgcctata actccctcta aagtatcata
     5641 tggtctacca gtaacagata aaatttcatc tcctggtcta actatacctt gtatacataa
     5701 agtaagtgca tgtgtaccat ttacaattat tggtcttaca agagcatctt ctgtattaaa
     5761 tactttagaa taaatttcct ctattttttc acgaccaata tcattatatc catatcctgt
     5821 agtccagtta aagtgcatat cacttagttt agattcttgc atagctttta aaactttata
     5881 ttgattatat tctcttattt cttttatttc ttcaaactta tcttttattt cttccattat
     5941 ttcttgcgat agtttaaaag tatcatcatc tattccatag tagtctttta ataattcttt
     6001 tgtctcatta agcattttat cacctgtctc tttattattt tatttaaaca tgtaaaggag
     6061 actattttac taaaacaaat aaattatagt ttataagtta gtataagtct cctctcaatc
     6121 atcttaaatt aaattaatta tctatctgtt gttattatta ttgttgtttt ggttttgatt
     6181 tgttagatta atatactttc caggttgtat agttgataca gcatgtttat aaatcatgtt
     6241 ttgttgtcta ttatcccctt ctattaatat tatataacta tcaaacccct ttacaagccc
     6301 tttaacttgc actccattaa ctaaatatat agtaacaggt atcctttctt tccttgcatt
     6361 atttaaaaac aaatcttgta aatttaaaac tgtatttttc attagatttg taccctccca
     6421 agattaaatt gaacttctaa aatattccta ccttatattc tatataagtt atgacaaatt
     6481 ttcaatatta agttgtatac ttaaaaattt atatattatt ttatagaatc tttaatatat
     6541 aagattattt cattttttaa ttcgtctata ttttcatatt ggtctaaatc aaaccatctt
     6601 gctgttttat atcttttaaa ccatgttatc tgacgttttg cataccttct agaatctctt
     6661 ttaattattt caatagcttc ttcgtatgta tattctccat ccagatactt caatatctct
     6721 ttataaccta taccttgcat tgatatcata tcttttttaa atcccatact taaaagtttt
     6781 tttacctctt caaccaatcc atttttaatc ataatatcaa ctctcatatt tattctttga
     6841 tacaaatgtt ctctatctct atttagaact attattattg gttgatattc ttcattaaat
     6901 tttaaatcac ttgaaaaatc attcattttc ttaccactta gacaaacttc aagagctctt
     6961 atgacccttt tagtattgtt tttatggatt ctattagcag attcttcatc aagttctttt
     7021 aacttattat gcatgtaatc tattcccttt tcagcaagct gtttttgaag ttcttctctt
     7081 aattcattat tggcatcaga ttgtgcaaaa tccatattat atattaaaga gtttaaataa
     7141 agtcctgtgc ctccagttac taaaacacat tttccctttt catttatttc atgtatatat
     7201 ttttttgctc tttgttgaaa ctctgaaact gaaaatggat agtcaggctt aacttcatct
     7261 ataaggtaat gcttcactcc ttgcatttct tcctctgtaa cttttgcact tccaatatcc
     7321 atatattcat atatttgcat agaatcagct gaaattatct ctgcatctat atcttttgct
     7381 aattttattg acaaatctgt ttttccaaca gcagttggac ctgtaagtat tataagtggt
     7441 atttttttca taacattcct ttccatatat tttaaatatc caaatatttt acataattct
     7501 tttaaacatt ttttctattt ctgtcttaga tatttcgacc attataggtc ttccatgagg
     7561 gcatgtaaat ggattctcac acttttctag ctgttccaat aaacttttta tttcaatatc
     7621 gtatatttta tcatttgctt ttatagcaga cctacatgcc atagatgcaa atctctctcc
     7681 ttttaaatca taattacttg taatttcttc tatattatca attatttgaa gtataaattt
     7741 ctcagtttca ggtactccaa atatagtagg aacacatctt accattatat gattattgcc
     7801 aaaaatttct agttcaaaac caaatttcat aaataactcc aagttatttt caatttgaag
     7861 catatctaca tttgatactt caataaccac tgggtctaaa agtatttgca tattgatatc
     7921 ttgtctatag aacttctcca tatatctttc atatagtatt ctttcatgtg cagcatgttg
     7981 gtccaacaaa tacatagaat cgtctttgct caaaattata taagtattaa atacaactcc
     8041 aattacagaa tatccatata aggaaaactt gcgttttgaa ctacacaagt attcttcttc
     8101 actgtcctta atactatctc ctaagtaata atttccttca tttttaattc catcaacttg
     8161 aaactcttct tgtatattat catttgcact atcttcactt agataattaa tgttttttac
     8221 atcatcttct acacttgcat ttaggacttc acttactgat tgaaattcat ttattggttt
     8281 ttcacttgat aaagtcacaa cttcaatgac ttcatcctta tctttagtaa tattactatt
     8341 tttaggagta gattctaata aatcattatt tcttaattta taatctgtac ttttttctct
     8401 actgttaata gcgattctag gttgtgtttt tttatcacta taagtagcat attttccaat
     8461 aagatttgag tgtattaatt taacctttaa aaaatctcta agttctatat acacttcttg
     8521 ttctttttca aatttaattt caagtttatt ggggtgtata tttacatcta tacatgatgg
     8581 gtctacttct atatttaaga agcacactgc atgtttacct attggaatta tagacttata
     8641 actttctgtt attgcatcta tgattatttt actttttaca aatcttttat ttatatagat
     8701 atgttgtaag tttttatttg aacggtatat attgttatta cctatatatc cattcatttt
     8761 aaagtgattg cacttaaact ctacatctat tatattttcc gttatttctt ttccatatat
     8821 acttctaata gtgtttacaa gttttccatc ccctggagta tttaacatct gtttattgtt
     8881 atttgtatat ttaaattgaa tgtttggatt acctattgca agtttattta tcaaatcact
     8941 tatgtttata gtttctgcat gagtagattt taaaaatttc tgtcttgctg gtgtattaaa
     9001 gaaaatatct tttataatga tggtagttcc attagtagaa cctattggtt cttttgatat
     9061 tatttttccg ccttcaacat atattttagt tcctattatt tcatctttag ttttagtcgt
     9121 catttctaat tttgatacag ctgaaataga tgccaatgct tctcctctaa aaccaagaga
     9181 gtataaatca tacaaatcat caattttttt aattttacta gtagcatgtc tcaaaaatga
     9241 tttttcaact tcacttgaag gtattcctat tccattatct gtaatcttaa ttagactttt
     9301 tcctccatca attatatcta tggaaatttt atttgcacct gcatcaatgg agttttcaat
     9361 aagttctttt actacagaag aaggtctttc tacaacttca cctgctgcta tcttatttat
     9421 agtcaaatca tctaggatgt taattatatt tttcattttt acacctctaa gattttattt
     9481 cttttgcttt tctttgaaga ttataaagag agtttatagc atctaatgga gtcatattta
     9541 aaatatccag atttaaaatt tcttctgata gtatatctct atttacacta tcaaaagaga
     9601 tttgagttaa ggctacttct tttacagaat cttcttgatt aattttctct ttatcatcat
     9661 tttgcttcat taaagcaact tcatcattta atgctttaaa tttagaattt aatttatcat
     9721 aatccttttt tatatgtttg tagtctttta ctataagttc atgttctatt tttagagatt
     9781 catatttatt cttaaaatta ctttgattta ccttaactaa ttcttcgtcc aaattactat
     9841 taattatatt attgttttct ttatctccat ttattgcaac actgttgtaa acatgatttt
     9901 tttccaagtc tttaagtata tattttgctc tttctatgac ttcgtctggt aatttagcta
     9961 atttagcaac atatattcca taactcttgt ctgctccctg aggaattatt tttcttaaaa
    10021 atattattcc ttccccatct tctttgactg ctattgaata atttttaacc tctttaaact
    10081 cttcttctaa atcagtaagt tcatgataat gtgttgcaaa taatgtctta catctaatat
    10141 ttttttgaat atattccact attgaccatg ctaaacttat cccatcatag gtactagttc
    10201 cacgacctat ttcatctaat ataactaaac ttctctctgt tgcatttttt aatataaggg
    10261 atacttcatt catctctacc ataaaagtag attgaccttg agataaatcg tcactagctc
    10321 caactcttgt aaatattcta tctaatattg gaatatcagc agactctgct ggtacaaatg
    10381 agcctatatg tgccataaga gctattatag cagtttgtct catatatgtt gatttacctg
    10441 acatattagg accagttatt atgttaataa tgttttctcc acgatttaaa taagtatcat
    10501 ttggaacgaa attctcctct cctactatat tttcgacaac aggatgtcta ccatttctta
    10561 tatcaagttt attattttca tttattgctg gttttacata gttatttata tgtgcaactg
    10621 ttgcaaggga cacaaaaaca tctatattag ctatagtctt agccactttt tgtattctat
    10681 ctatgttttt atatattgta tctcttatct ctacaaatat ctcgtattct aaagatttaa
    10741 tcttttcctc tgcatgtagt atcttttctt ctatttcctt taattctgga gttatatatc
    10801 tttctgcatt actaagtgtt tgctttctta tatatgtctc atctagcttt gcctgtttaa
    10861 agttggcttt tgttatttct atataatatc caaaaacttt attaaatcct atttttaatg
    10921 atttaactcc tgttttttct ctttccctgt tttctatttc ctttacaaga aaggctccat
    10981 tcttagaaat ctctcttagt tccttaagtt catcactaaa atctgattta attatgtttc
    11041 catcttttat ggttatcgta ggttcttcta gaatagcttc atctattaaa ttgtatatat
    11101 catctaattt atccatttct gaaacatact cttttaggat ttttgcattt gataaattta
    11161 tagtatcttt taaattaggt aacttttcta tcgaattttt taagtgaatt aactcttttg
    11221 gtgttactct ctcaaatgct atctttccac atattctttc tatatcgtat atatttttaa
    11281 gtatatcatt taggtcttct cttaatataa agtcatcttt tatttcttct attacatcta
    11341 atctattttc aattttagat ttatttatca aaggttcttc tacatatttt cttaaaagtc
    11401 tacctcccat tgctgtagaa gttttatcaa gtacatgtaa taatgaacct tttttcttat
    11461 tccctcttat agtttgagta agttctaaat tagttctagt aaacatatct aaaaccatat
    11521 attctgatga attgtatata tttatattat taatgttaga tgttatttgt ttttgagtat
    11581 tgtatatata attcagaaga attgataagc ttgactttat tagaccttta tcatcaaact
    11641 ttagtttttg tagataaaca tctggaaaat attcttttaa tatatttata tccaaatagt
    11701 tatcactaaa actttcattt atgtatatat tacttacagt agctatatct ctaagctttt
    11761 ctataaagtc taaatcattt ataattattt cagttggatg gatttttgca atttcttcaa
    11821 ttaccttatc ttcatttaaa catgttgcat tagtttctcc tgtacttata tcaacataag
    11881 ttagacctat attagtacca tccttataca aagataataa ataattattc tttttatttt
    11941 caagtaaatt tccatctaaa actgtaccag gagtgatgac tcttataacc tctcttctaa
    12001 caattccttt tgctgtagat gggtcttcca tttgttcacc tattgctact ttatatccat
    12061 tttcaactaa ttttgatata tatgaattgg ctgagtggaa aggaacacca cacataggag
    12121 cacgttcttc taaaccacaa gcttttcctg tcaatgctat ttcaagagct tttgaagcta
    12181 ctaatgcatc ttcaaaaaac atttcataaa aatctccaag tctaaaaaac agtatacagt
    12241 ctttatatct attttttact tcaagatact gtttcatcat aggagttaat ttattcatat
    12301 ctatttgcat tatcaaacct ccttcttttc taattaaata ttatatcata gtagatgatt
    12361 attattaaca caatatataa aattttgaca tataactaaa aacaatccaa aatgtatact
    12421 cacattttgg attgctaagt ttaatgctta ttaaataaaa atatatatat ttttacaata
    12481 aagatattat ttctttttta agtttaatga tattttcaag gttatcttca tttaactttc
    12541 tactatctat aacaaattct ttctttacta ttgatggttt ttttgaaata acataaattc
    12601 tgtttgatag cattatagct tcgtcaatat catgagttat aagaagtatt gttgaattta
    12661 cttttttctt aacatctaaa agccattttt gcatggatgt tttagttaat gaatcaagtg
    12721 ccccaaaagg ctcatctaaa agcattatgt cattagaatt tataaaagtt ctcaagaagt
    12781 tagctctctg tctcattcct ccagaaagtt ctgaaggata tttatcttca tatccgctta
    12841 atccaaacac ctcaaaataa ggttttacca ttgacctagc tttactcttt ttttcttttt
    12901 ttaaaattag tggtagtgac acattatcta ttatagtttt atatggcaag agtaaatctt
    12961 tttgatacat ataactatat ttaccattta tattcacttt gccactatca taatctgtaa
    13021 gtttagttat aatattaaaa atagtacttt ttccacatcc acttggccct aatatagaaa
    13081 caaactcacc ttcataaaca tcaatactta tatcttttaa aacttcaata tctttataag
    13141 tttttgatac attttctata gatactttta cttttttatt gtggtaaaaa ttcatttgtg
    13201 taagcatcac ttgccttcat atccttatct attaatttat attcttttaa gaatgatgta
    13261 tagttgttcc atacactatc tttcatttct ccccatcgtg gtgcatcatc tttgtacttg
    13321 cttgctaaat attcttgact ctttaaagct agtttttcat ctacttcagg tgcatgtttt
    13381 actaaaattt ttgctgattc ttctggattt tttattgcat attcataacc ttttgttgta
    13441 gcttttaaga attttttagc aagctctgga ttatctttaa gcacagtttc attagaaatt
    13501 ataagtggtg tataataatc caatctctca tctaaatctt ttactggtat aaagtttaag
    13561 tcatatccaa ttaaatcagc tttaactgca tcccaacctt caaatatcca tgcaaaatct
    13621 acagttttca tagctgcaaa gaaatcatct tgaccagtat taactatctt taacttatta
    13681 aaatcagctc catttttctt cataactgct ttaaaaacag cttcttctga tggagagccc
    13741 caaccaccat aagtttttcc ttcaaaatct ttagctgttg ttatattctt ttcttttggt
    13801 gatgcaaacc cagaagtatt atgttgtata acagttgcta cagcttttat tggaagtgga
    13861 tcttcactag tctttgcata agtaacttgt tcttgatagc ttataccaaa gtcagctttt
    13921 cctgttgcta ctaaagtttc tgctccactt tcaggtggtt gtactatttc aacatccaaa
    13981 ccttcttctt tatagtaacc cttatcaagt gctacaaata atccagtatg atttgtattt
    14041 ggtgtccaat caagcaccat agttactttt ttagaagaag tatctttatc attgtttttg
    14101 tctttattgt tatttgaaga acatccagtt attgttgcca atgtaaaaac acttactaaa
    14161 cctaatattc ctaacttttt aaaattcata attgcctccc tcaaatacct ttaatcaatt
    14221 aatctatgtc ttataacaac tttttttaca gcctgtacaa ttcctacaaa cattaaacta
    14281 aatataacta ctaatatagt agatgcaaat actttatcaa gtgcatatgc actttttgac
    14341 ctaagcatat aaacaccaag tcctttcgtt ccacctagcc attctgcaac agttgctgcc
    14401 attacagcat acgttgcaga tattttaagt ccagaaaaaa atttatccat tgccatagga
    14461 aatttaaggt gaatgaatgt attaacttta tttgagtcca tagtcttaaa taaatttaaa
    14521 taatctttat caatattctc aatgccatct acaaaactta ttaatatagg aaaaaaacaa
    14581 gtaagcatta ccatcaatac ttttggcatt gttccaaatc caaaccatat cataaaaagt
    14641 ggtgctataa ttattgtagg tatcatctgt gtaaccaaca taataggata aagacatttt
    14701 tttattattg atacaaaatc catcaatatt ccaattacca atgatattat aattgcgact
    14761 ataaaaccaa gtattgcttc tcctatagta accacagcat gcatggataa attttgataa
    14821 tctttaaaaa acacattaat tatatctact ggtgtgggta gtatatactg aggaacttct
    14881 aaaactccta ccatagattg ccaaagaata agtataaaca aaaatgttgc tattggatat
    14941 atactatctt tgactttatc tatgatttct aagtttttca tctatagtca gacctccagc
    15001 tttttcatct actctagcta ctgttatgac tctttcacat ccaagattaa aagggatttt
    15061 atgtaccttt tttaataatt caaaaacttc atcaaaatca ccttcaacag tagtgctcat
    15121 tgctcctatt tcacatttaa gaccactttt ttcaatttca gcaatacaag catctactac
    15181 tttatacatt tcttcttcac ctttgtaagg tcttagtggc attactgcta tgtctgcgat
    15241 taccatttaa atcactcctt taaaaataat taatacctac taggtatatt ttgtttttta
    15301 ttaaaaaatt ttattactaa aaagatatta tatttaaata aaagaaaagt cttatccaaa
    15361 ataggataag acttattttt atcttaactg gtctccctac actggcatta tccaaacagg
    15421 ttcaaagggt cgagataaaa tctcctctca gcacattagc tccccgaaat atattatatc
    15481 ttttctactt gattatacta cacttatcat attttaacaa attttttatt tatattagat
    15541 attttatccg ttcaaaaata tctaatatac attgtacatt attacaccaa ataaattatc
    15601 ttaaaatggt aatttatcaa catcttccaa aactagatta actgaatttt ctagttttta
    15661 ttttgctatt tcaagcatat ttggatgtat atcaattcca tacaaatatt gttctaaatt
    15721 tctgttttca ctaatctaat ttgcaatcgt tacagattac agattagaaa tagcttttag
    15781 actctactta tctttatcta caaaaaatcc ttcaataaat tcagtaataa tattcagtaa
    15841 tctaaaaata aacctctatc tggattttta agatacaagc cttttaggaa gtatcttttg
    15901 tgataatata aaaattactc gtcattatca tcaaaatcct cagcaaccaa cattttagat
    15961 aagaatcaaa ctctataata taattatttt atccaattta tttttgagaa ataaaatatt
    16021 tttttaattt ttaaattttt taatatgcac cttgaaaaaa gaaacgtttt cctatattat
    16081 ggtaatagta gaataaaact aaataggcaa aactagagaa atttagtgac gcaaagctat
    16141 agggactaag acttatgaaa tgagttatgt cagccagttg ccaaagagat attttctttt
    16201 tgtttttatg aatgtttttt agggggatat atgatatatg tttatggata aaatggatag
    16261 atgtacacat atactaactg catatatatg cagttcacgc gattactgca attttataga
    16321 tacacaatta aatgatttta tactagaata tggagagaat gtggtagaat cttgtttgca
    16381 tcaagtgatg gtattggtaa gcagatataa ttaaagaaat tttattttct agaaaaccct
    16441 ttttagtttt atctacgttt tataattcat taaagaacta atcaaaataa gcttcattat
    16501 atatttttca tgaagtaaac agttgagttt atctcatata ctgtttaccc caattacata
    16561 attctaataa tatagattcc aatgatttac cacgtttagt agacaaatat tcaacttttg
    16621 gaggaatagt cggataagaa attctcttaa taagtccatc tctttcaaga tactttaatt
    16681 cttgtgacag tatcttatga cttatatgaa caacatcttt ttttaattga ctgtaacgta
    16741 ttggctcttg ttttaaaata tgccataaaa taattgccct ccatttactt cctatcacag
    16801 tcatggtaaa accaattgaa caattaaaat ctttttgtct ttcttcaata tttggaaatt
    16861 cttcaaaatt attatctata tccatctcaa tcctctcttt caaaatagta agtatctaac
    16921 taaaaagtgc attcttgttt agcgtactaa ttcgtaatat aatcttgatt aaaaaatagc
    16981 tacgcacact cgtgacttta gtcatgagtt agtagctaaa atagtcaaca tatagggaaa
    17041 c
//
LOCUS       DAMJWP010000063        16777 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.065, whole genome shotgun sequence.
ACCESSION   DAMJWP010000063 DAMJWP010000000
VERSION     DAMJWP010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16777
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.065"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            188..364
                     /locus_tag="PC700_001751"
     CDS             188..364
                     /locus_tag="PC700_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581063.1"
                     /translation="MEINVIEIFPKDKAKLNKIEMDKASWFVNIISKKYPKEALNEAF
                     SILEKELNISKANT"
     gene            538..1668
                     /locus_tag="PC700_001752"
     CDS             538..1668
                     /locus_tag="PC700_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDF2581064.1"
                     /translation="MNNFEIKTRIKFGEGSLKVLQELKNKNVLIITDPFMVKSKTIDK
                     ITSNLINSTYQVFEEIVPDPPIEVVVAGIEAFKSINPDVIIALGGGSAIDAAKSIMDF
                     SKKILKINEVEFIAIPTTSGTGSEVTSFAVITDSQKKCKYPLVSDDLLPDMAILDPEL
                     VKTVPNFITADTGMDVLTHAIEAYVSTNSNDFSDALAEKSIKLVFKYLIKAYKDGNDI
                     EAREKMHNASCLAGMAFNQASLGINHSIAHVLGGKFHIPHGRTNSILLPHVIEYNSSI
                     VGYTNTTYSDTAKKYAQIAELVGLGSANIRISVRNLVNEIKKLQKEMNMPTKLTQCKL
                     SLEDITNEEREIAKLAIKDTCTLTNPKVPTEDDIINILNNIK"
     gene            1909..2259
                     /locus_tag="PC700_001753"
     CDS             1909..2259
                     /locus_tag="PC700_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HDF2581065.1"
                     /translation="MTEESKQRVIQEYVPGKQVTLAHIIANPNEDIYKKLGLVLDKRD
                     AIGILTITPSEASIIAADVATKASNVSLGFIDRFSGSVVISGDVSSVESALNDVLEVL
                     GNMLNFSSTKITRT"
     gene            2267..2716
                     /locus_tag="PC700_001754"
     CDS             2267..2716
                     /locus_tag="PC700_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HDF2581066.1"
                     /translation="MKNIIFMGKTGSGKTTLCQKLDQLELKYKKTQSVELYNNAIDTP
                     GEYMENRNLYNALITTSADAKIIAIVYDATQEENYIAPGFASMFCKDVIGIITKINKA
                     TENEVNIAYERLSLAGASQIFKIDTVDDIGLEKLFKYLEKSKNIDEV"
     gene            2818..3393
                     /locus_tag="PC700_001755"
     CDS             2818..3393
                     /locus_tag="PC700_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDF2581067.1"
                     /translation="MGKKILLVDDEPLLRMDTKDILESHGYEIIGEASDGFEAVEMCK
                     KHKPDLVIMDIEMPILDGIKAGKIICKENLAGGVLLLTSFEDKEYVEKAKSIGAYGYL
                     VKTASEKSLIPTIEMCLNKVNEFEKMKKDLEKVNSKLNERKLVERAKGILIREFKISE
                     DEAYTRIRKLSMDKRTTMMEIAKMIIIGYDD"
     gene            3386..4795
                     /locus_tag="PC700_001756"
     CDS             3386..4795
                     /locus_tag="PC700_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HDF2581068.1"
                     /translation="MTNTIKKISTAQTYLNNNEIEKIIEVSKSLDLMASFYEADVFID
                     VLSKFDDEAIVVAHGRPKEKSMYCENVIGKKALIENEPGVIKCLKTGERVRDIKALTQ
                     EYKLVKQNIQPITLDDKVLAVLIVEKDISSEVKNEFNASEDEENKNLHQFIKLIGNND
                     FLVDNLNSAILIFDKNGKLKIKNRIATEIYKNLGFGYDIQDMHYNDLDIETNTFENIL
                     MDENYDETNEVSIGNMFFKIKTIYIKDDEFKVCKIVQDISDLKRKEEELVLKTVAIRE
                     AHHRVKNNLHTVISIIKKQSRLSQNDEVKQCLDNIINRVFAILSTHYLLSKEVDNNIS
                     IREGINLLVSNIQGGYLFSKDIDIRITGEDFKISGEKATAILLVINEILQNCYDHAFS
                     DREHGTIQILINKDNDYRNIAIIDDGIGFDSNNINKNSLGTYIIDSYITQMLKGNIER
                     KSNENGTEVLITIPTQINI"
     gene            4979..6412
                     /gene="eutA"
                     /locus_tag="PC700_001757"
     CDS             4979..6412
                     /gene="eutA"
                     /locus_tag="PC700_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HDF2581069.1"
                     /translation="MREELLSVGIDIGTSTTQLIFSKLIVENTESNFSVPRISIVDKE
                     IIYKSKVYFTPLISNNEINGQQIKDIVENEYKKANISKEDIHTGAVIITGETARKENA
                     NDVLHTLSGFAGDFVVATAGPDLESIISGKGAGAHIYSKEHSTTIVNIDIGGGTSNIA
                     SFKRGEVTDTGCLDIGGRLVKIDKISKKITYISPKIDKIIKDNNLNLQVGTMATVENT
                     KPIVDIMVDLLMESIGLKKQTPLFDFIITNKSISIDENIKNVSFSGGVADYIYYDGEI
                     KDYFEYGDLGILLGQTIKNCEYLKKVNVVKSMETIRATVVGAGSHTTEVSGSTITYTS
                     EKFPLKNLPVLKLSVDEETGTSKQISEAIKNKLNWFKLENDLQKVAIAMIGKKNPTFL
                     DIQEYAKGFVDGMQQIIENDLPFIVIVENDMAKVLGQAIYALLNYEKEIVCIDSVKVE
                     NGDYIDIGKPIVEGKVLPVVIKTLVFN"
     gene            6430..7794
                     /locus_tag="PC700_001758"
     CDS             6430..7794
                     /locus_tag="PC700_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HDF2581070.1"
                     /translation="MILNTKLFGRTYQFKGLYDVMAKANEEKSGDKLAGLAAESAEER
                     IAAKVVLSHIKLEDIRNNPAVAYEEDEVTRIIQDAVNEKVYEEIKNWTVSELREWLLD
                     SNTTSLDIRRISRGLTSEMIAAVAKLMSNMDLVYAAKKIKVTAHCNTTIGEQGTLSAR
                     LQPNHPTDDPDGILASLLEGLTFGIGDAVLGLNPVDDSVESVTKVLKRFEEVKQKYQI
                     PTQTCVLAHVTTQMEAIRNGAPADLIFQSIAGSEKGNEAFGFNGDTIEEARQLALKHG
                     TATGPNVMYFETGQGSELSSEAHHGIDQVTMEARCYGFAKKYEPFLVNTVVGFIGPEY
                     LYDAREVVRAGLEDHFMGKLHGLPMGVDVCYTNHMKADQNDMENLAILLTTAGCTYFM
                     GIPHGDDVMLNYQTTGYHETASLREMFGLTAIKEFDAWMEKMGFYKDGKLTELAGDAS
                     VLLG"
     gene            7807..8688
                     /gene="eutC"
                     /locus_tag="PC700_001759"
     CDS             7807..8688
                     /gene="eutC"
                     /locus_tag="PC700_001759"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014952556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HDF2581071.1"
                     /translation="MNEKDLKALVEQLVGQMVGELDTNVVSETVKKTTEVVVDNNAFI
                     DDITEVDIRKQLLVKNPKDAEAYLDMKAKTPARLGIGRAGTRYKTETVLRFRADHAAA
                     QDAVFSYVDEEFIKENNMFAVETLCKDKDEYLTRPDLGRKFSQETINNIKSKFGTNQK
                     VLILVGDGLSSAAIEANLKDCVPAIKQGLKMYGIDSSEILFVKHCRVGAMDHLGEELG
                     CEVICMLVGERPGLVTAESMSAYIAYKPYIGMAEAKRTVISNIHKGGTTAVEAGAHIA
                     ELIKTMLDKKASGIDLK"
     gene            8708..9361
                     /gene="eutL"
                     /locus_tag="PC700_001760"
     CDS             8708..9361
                     /gene="eutL"
                     /locus_tag="PC700_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HDF2581072.1"
                     /translation="MKNDVIRPNILGIKIISNISPEMAQKLELKSHHKSLGLITADCD
                     DVTYTALDEATKASEVDVVYAKSMYAGAANASTKLAGEVLGIIAGPSPAEVRSGLNAV
                     VDFLEYGATFISANDDDSIAYYAHCVSRTGTYLSEIAGIREGEALAYLVAPPLEAMYA
                     LDAAMKAADVKMCELFAPPTETNFGGALLTGSQSACKAACDAFAEAVKSVADNPTGF"
     gene            9372..10073
                     /locus_tag="PC700_001761"
     CDS             9372..10073
                     /locus_tag="PC700_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HDF2581073.1"
                     /translation="MDAIGLIEVIGYVAAIEASDACLKSANVNIVRIDKVGAGIVTLT
                     ICGDVGAVKAALEAGEIAASRVGTLRTSHIIPRIHNEVTNALFKTKEPKVCEVSEDKE
                     KNFEITLEITDNVSKNLSEKENVILDVNENSGDTENLYSDIDKTNKKIETVDDSLTEA
                     NEEKIENTNDKEVLEQVKIENSDFDRDDLSKNSVKKLKAMAKKLDSSITYKDLNVLKK
                     EELINLVKKLSRRDR"
     gene            10076..11545
                     /locus_tag="PC700_001762"
     CDS             10076..11545
                     /locus_tag="PC700_001762"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HDF2581074.1"
                     /translation="MTLLDKDLVSIQEVRTLVRRSKVAQEKLSKMSQEQIDKIVKAVV
                     DAAFNNATKLAKMANEETGFGKWEDKVIKNTFASMGVYETLKNTKTIGIIGEDKEANT
                     IDIATPVGVVAGLIPSTNPTSTVIYKTIISLKAGNTIVFSPHPNAKNCILETVNIISE
                     AAIKAGAPEDSILCITNPSIVGTNELMKHKDTNLILATGGEAMVRAAYSSGTPAIGVG
                     PGNGPAFIDKSANVKLAIKRIIDSKTFDNGVICASEQSIVIEKSMEETVLKELKNQGA
                     YVLNEEESERLAKFILRANGTMNPQIVGKSVEVIANLADLTNIPKNTKVLVARENKVG
                     HNVAYSREKLTPILAFYVEENVEAIMNRCREILLNEGAGHTFSMHVNDEELVKRFALN
                     MPVSRILVNTPSALGGIGASTNLVPALTLGCGAIGGSSTSNNIGPLDLINVKRVAYGI
                     KEIEELGRDKSQVEKSMFDSMDKEELINLLVQKLVKELA"
     gene            11641..11928
                     /gene="eutM"
                     /locus_tag="PC700_001763"
     CDS             11641..11928
                     /gene="eutM"
                     /locus_tag="PC700_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HDF2581075.1"
                     /translation="MASANALGMIETKGLVGAIEAADAMVKAANVQLVGKEQVGGGLV
                     TVMVRGDVGAVKAATDAGAAAAERVGELISVHVIPRPHFEVDAILPKVSAE"
     gene            12055..12816
                     /locus_tag="PC700_001764"
     CDS             12055..12816
                     /locus_tag="PC700_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin adenosyltransferase"
                     /protein_id="HDF2581076.1"
                     /translation="MGVLTENEVKRKIGKNKNNITEFYIEKGQIITPSAKSYLAERSI
                     ELKYVDKLSDVNNEPKEVVKEVVKEKKYKYTTVFGAKLDEKPEHMTQLNGNLLVFKDH
                     KRIILRGKIDSLESEILKVQILCQKLDMPKLVKELQEVLDFVRNIMRCEVLDEKLEDF
                     KLLGLTPQELREQSHYPKKYFGIGHEFPSYEMGEVVVAINSIRSNTREVELAAYEAFK
                     GEYGNVHREDLIKALNRLSSVFWIMIYKVRTNKYK"
     gene            12829..13458
                     /locus_tag="PC700_001765"
     CDS             12829..13458
                     /locus_tag="PC700_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HDF2581077.1"
                     /translation="MENLLNEIVEEVITRVKKEAFIEVEASGRHVHLSREDVDKLFGE
                     GYTLTKLKDLSQPGQYACKERVTITGPKGSIKNVVVLGPCRNETQVEISLTDGSTLGL
                     KAPIKQSGDLEGTLSIKISTQYGEVELDKGLMVAKRHIHMTPKDAEKFNVCDKEIVEA
                     KVMGQRPLTFDDVVIRVSDSFKTYMHIDYDEANACGYSKGTVAKIIKKA"
     gene            13500..14225
                     /locus_tag="PC700_001766"
     CDS             13500..14225
                     /locus_tag="PC700_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02536 family ethanolamine utilization
                     protein"
                     /protein_id="HDF2581078.1"
                     /translation="MNYDNLVDLITEEIYKKINSNEIKISNKPKAVIVFEQDNNKFNL
                     LKDEFEILEFDKSIRECEIVIVSRLCMRGLSNIALGNSTSDEERFILKMIMKGKKVYV
                     LDEGIEYKKYKDTAPKALYKKFMSFEDEICKFGVEIIKDLNVITKNKLNIKKELESTR
                     GSKKEDTKVLDKDVLNLTSVESVKIDNEFSLDLRNKKLISEADLRKPTINGVKNILVN
                     KKSIITPLAVDFMRIHHLKLKKL"
     gene            14238..14510
                     /locus_tag="PC700_001767"
     CDS             14238..14510
                     /locus_tag="PC700_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HDF2581079.1"
                     /translation="MDIGKVVGNVWATRKDEKLCGQKLLVVKILKTKDTYKEGFMVAA
                     DNAGAGNGDLVLISKGSSARESIEKSHVPVDATIVGVVDSFEVFDE"
     gene            14503..15054
                     /locus_tag="PC700_001768"
     CDS             14503..15054
                     /locus_tag="PC700_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HDF2581080.1"
                     /translation="MNKSIGAIEFKSIAKGIEVSNEMIKKSSVDVLYLKSICPGKFLI
                     IVGGETSYINECVDYGIKLGEGYIVDNFVINAISQEILDGFKNKYQKLDSIVSIGVVE
                     SSKVCAGIKMLDKTLKSGDLVLVKLQLSFAIGGKLVYVVAGDLSSLEYALKESENVVR
                     EKEVIYKTVIPSVDSQIIKSLIK"
     gene            15066..16157
                     /gene="eutH"
                     /locus_tag="PC700_001769"
     CDS             15066..16157
                     /gene="eutH"
                     /locus_tag="PC700_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HDF2581081.1"
                     /translation="MSINEIIIYVMVVFMVLGAIDKCIGNKFGLGEQFEEGIMAMGSL
                     AVAMVGVICLAPVLADVLRPIVVPVFNILGADPAMFAGSLLANDMGGAPLALELAKDP
                     NSGLFGGLIVGAMMGPTIVFIIPVALGIIEKEDQKFLATGILAGIITIPIGAFVGGLV
                     AGFEVMMVLRNLVPIIIFAVVIALGLLKFENAMIKGFTYFGKGVVIVITLGLAAAIVE
                     ALTGIVVIPGMKPISEGIEIVGDIAIVLAGAFPLVFVITKVFNKPLMALGKVLGMNDV
                     SAAGLVASLANCIPMFGMMKDMDNRGKIINVAFSVSAAFVFGDHLGFTAGFNSNMITP
                     MIVAKLVGGITAVLLAMVIANKTLRKENV"
     gene            16159..16632
                     /locus_tag="PC700_001770"
     CDS             16159..16632
                     /locus_tag="PC700_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF861 domain-containing protein"
                     /protein_id="HDF2581082.1"
                     /translation="MDISNIDKNLIETLVRQIIEEKISGTKDTVDFVRNKDISGITSI
                     KLPTVKVSESDRLDTGNPSDVVYTKDLFTLEESPRLGCGMMEMKETTFDWTLNYDEID
                     YVIDGTLDIIIDGRKVSASSGELIFIPKGSKIQFSVPDYARFIYVTYPADWASQN"
BASE COUNT         6922 a         1875 c         3115 g         4865 t
ORIGIN      
        1 ttctttacta ttcaagttct aaatcaataa taaattttga tatttaattg tatcaggaca
       61 aatctctaat ttcatatatt attaatataa aatcataggt tagtgtgaaa aagttgaaat
      121 ttaaatattt taaaagataa ttttttggaa ctaataggta taacaaaaat ttataggggg
      181 attgcatatg gaaatcaatg ttatagaaat tttccctaaa gataaagcta aacttaataa
      241 aatagaaatg gataaagcta gttggtttgt aaatataata agtaaaaaat atcctaaaga
      301 agctttaaat gaagcattta gtattttaga aaaagaatta aatataagta aagctaatac
      361 ataagctttg attattgatt ttatatacta ttggttttat acgctatgac ttgacattat
      421 attaatcaaa tagtatattt ttaaatatag cacaatgaag tgttaaaaga gcaatggagc
      481 tccatagata aagtctatgg agcttttttg cgtatttaac ttaaggagga tagagctttg
      541 aataactttg agattaaaac tagaataaaa tttggagaag gttcactaaa agttttgcaa
      601 gaattaaaaa acaaaaatgt attaataata acagaccctt ttatggtgaa atcaaaaaca
      661 atagataaaa taacatctaa tttaataaac tcaacatatc aagtttttga agaaatagta
      721 ccagacccac caatagaagt agtagtagca ggaatagaag catttaaatc tataaatcca
      781 gacgtaataa tagcattagg tggtggttct gcaatagatg cagcaaaatc tataatggac
      841 ttttctaaaa aaatattaaa aattaacgaa gtagagttta tagcaatacc aacaacaagt
      901 ggaacaggtt cagaagtaac atcatttgct gtaataacag atagtcaaaa aaaatgtaag
      961 tatccattag tgagtgatga tttacttcca gatatggcaa tactagaccc agagcttgta
     1021 aaaactgtac caaattttat aacagcagat acagggatgg atgtattgac acatgctata
     1081 gaagcttacg tatctacaaa ttctaatgac ttttcagatg cactagctga aaaatctata
     1141 aagttagtat ttaaatactt aataaaagca tataaagatg gaaatgacat agaagctcga
     1201 gaaaaaatgc acaatgcatc ttgtttagca ggtatggcat ttaaccaagc atcacttggc
     1261 ataaatcatt ctattgcaca tgtattaggt ggaaaattcc atataccaca tggaagaaca
     1321 aattctatat tacttcctca tgtaatagaa tataattcat caatagtagg atatactaac
     1381 acaacttact ctgatacagc taaaaaatat gcacaaatag cagaattagt aggacttggg
     1441 agtgctaata taagaatatc tgttagaaat ttagtaaatg aaataaagaa actacaaaaa
     1501 gaaatgaata tgccaactaa attgacacaa tgcaaattaa gtttagaaga tataactaat
     1561 gaagaaagag aaatagcaaa acttgctata aaagatactt gtacattaac taatcctaag
     1621 gtaccaactg aagatgatat aataaacata ttaaataaca ttaaataact acattttatt
     1681 acatgtaaat ataatattat acaaataggg aaaatgtttt taaaaatata aattatatat
     1741 tgacaaatat aaaacataaa actataatga aagtatcaca aaggcgtgat actttagtag
     1801 aaagcaatgg agcttcttag gaatattaat attattctta tggagctcct tttattttaa
     1861 gcaaatagct ttttaataaa ctatattcaa agaaagcagg gaataaattt gactgaagaa
     1921 tctaagcaaa gagttattca agaatatgtt cctggaaaac aggttacatt agcacatatc
     1981 atagctaacc ctaatgaaga catatataag aagttaggat tagtacttga taaaagagat
     2041 gcaataggaa tacttacaat aacaccaagt gaagcgtcaa taatagcagc agatgttgct
     2101 acaaaggctt caaatgtatc tttaggtttt atagatagat ttagtggttc agtagtaata
     2161 tcaggtgatg taagttctgt agaatcagcg ttaaatgatg ttttagaagt cttaggaaat
     2221 atgttaaact tctcatctac aaaaatcaca aggacataat aatacaatga agaatataat
     2281 atttatggga aagacaggta gtggtaaaac taccttatgt caaaaactag accagttaga
     2341 attaaagtat aaaaaaactc aatcagtgga actttataat aatgcaatag atactcctgg
     2401 tgagtatatg gaaaatagaa acctatataa tgccttaata acaacatcag cagatgctaa
     2461 aattatagca atagtgtatg atgccacaca agaagaaaat tatatagcac caggttttgc
     2521 cagtatgttt tgcaaagacg taataggaat tataactaaa ataaataaag ctactgaaaa
     2581 cgaagtcaat atagcttatg aaagactatc actagcagga gcaagtcaaa tctttaaaat
     2641 agacacagtt gatgatattg gacttgaaaa attatttaaa tatctagaaa aatctaaaaa
     2701 tatagatgaa gtttaagggg aaatattttt ttattcaaaa atattattaa agtatgataa
     2761 aataaaaaaa tgtataaact attttaagaa gatttaatct ttaagcgagg taggaacatg
     2821 gggaaaaaaa tactattggt tgatgatgaa ccactgttaa ggatggatac aaaagatata
     2881 ttagaaagtc atggctatga aataataggc gaagcaagtg atggatttga agctgttgaa
     2941 atgtgtaaaa aacacaaacc tgatttagtt ataatggata tagagatgcc aatacttgat
     3001 ggtataaagg caggcaaaat aatatgtaaa gaaaatctag ctggtggagt acttttatta
     3061 acttcatttg aagataaaga atacgtagaa aaggctaaaa gtataggtgc ttatggatat
     3121 ttagtaaaaa cagcaagtga aaaatcactt atcccaacca tagaaatgtg tttaaataag
     3181 gtaaatgaat ttgaaaaaat gaaaaaagat ttagaaaaag taaattctaa attgaatgaa
     3241 agaaaattag tagaaagagc aaaaggtata cttataagag agtttaagat ttctgaggat
     3301 gaagcataca ctagaataag aaaattaagt atggataaaa gaactacaat gatggaaatt
     3361 gccaaaatga ttataatagg atacgatgac taatacgata aaaaaaatat caactgcaca
     3421 aacatactta aataataacg aaattgaaaa aattatagaa gtatcaaaat ccttagattt
     3481 aatggcttct ttttatgagg ctgatgtttt tatagatgta ctaagtaaat ttgatgatga
     3541 agcaattgtt gtggcacatg gtagacctaa agaaaagtca atgtactgtg aaaatgttat
     3601 aggtaaaaag gctttaattg aaaatgaacc aggagttata aagtgcttaa aaacaggaga
     3661 aagagtaaga gatattaaag ctttaacaca ggaatataaa ctggtcaaac aaaatattca
     3721 gccaataaca ctagatgaca aagttttagc agtactaata gttgaaaaag atataagtag
     3781 tgaagttaag aatgagttta atgctagtga agacgaagaa aataaaaatt tacatcagtt
     3841 tataaaactt atcggaaata atgacttttt agttgataat ttaaattcag ccatattaat
     3901 atttgataaa aatggaaaat taaaaattaa aaatcgtatt gcaactgaga tatacaaaaa
     3961 tcttgggttt ggatatgata tacaagatat gcattacaat gatttagata tagaaaccaa
     4021 cacctttgaa aatatattaa tggatgaaaa ttatgatgag acaaatgaag tttcaatagg
     4081 taatatgttt tttaaaataa agaccatata catcaaagat gatgaattta aggtttgcaa
     4141 aatagtacaa gatataagtg atttaaagag aaaagaagaa gaattagttt taaaaacagt
     4201 ggctataaga gaagctcatc atagagtaaa aaataatctt catacagtaa tatctataat
     4261 aaaaaaacaa agtagattat ctcaaaatga tgaagtaaag cagtgtttag ataacataat
     4321 aaacagagta tttgcaatac tttcaactca ttatttacta tctaaagaag ttgataataa
     4381 tatatctata cgagaaggta tcaatttatt agtgtcaaat attcaaggtg gctatttatt
     4441 tagtaaggat atagatatac gtataactgg tgaagatttt aaaattagtg gagaaaaagc
     4501 aacagcaata cttttggtta taaatgaaat actacaaaat tgttatgacc atgcattttc
     4561 ggatagagaa catggtacta tacaaatatt aataaataaa gataacgact atagaaatat
     4621 tgctattata gatgatggaa ttggatttga tagtaataat ataaataaaa atagcttagg
     4681 gacatatata atagatagtt atataacaca gatgttaaaa ggaaatatag agagaaaaag
     4741 taatgagaat ggtacagaag tattaattac tatacctaca caaattaata tttaaataat
     4801 aactcatcta caaagtagta gttttaattg agcaaagagg cttaaggtta ttaatagatt
     4861 aagttaattg aatgtattta taaaaattat aagtatatat tcaatttaac aggtatttat
     4921 tataacttta agcctctttg cttgtttaag acatacttaa attttaggag gtgagaaaat
     4981 gagagaagag ttattaagtg ttggtattga cattggaaca agtactactc agttaatttt
     5041 ttctaaatta atagtagaaa atactgaatc aaatttttcg gtacctagaa taagtatagt
     5101 agacaaagaa attatttata aaagcaaagt ttatttcacc cctcttattt caaataatga
     5161 aataaatggt caacaaataa aagatatagt tgaaaatgaa tataaaaaag caaatatatc
     5221 taaagaggat atacatacag gagcagtaat aataactggt gaaacagcta gaaaagaaaa
     5281 tgcaaatgat gtacttcata ctttgagtgg atttgctggg gacttcgttg ttgcaacagc
     5341 aggacctgat ttagaaagta taatatcagg aaaaggagct ggagctcata tttattcaaa
     5401 agaacattca actacaattg taaatataga cataggtggg ggaactagta acatagcctc
     5461 atttaaaagg ggagaagtaa ccgatactgg gtgtttggat ataggtggaa gacttgtgaa
     5521 gatagataaa ataagtaaaa aaataactta tatatcacct aaaatagata aaatcataaa
     5581 agataataat ttaaatcttc aagtaggcac aatggctact gtagaaaata caaagccaat
     5641 agtggatata atggttgact tattaatgga aagcatagga ttaaagaaac agacaccttt
     5701 gtttgatttt ataataacca ataaatcaat aagtatagat gaaaatatta aaaatgtttc
     5761 attctctggt ggagtagctg attatattta ctatgatgga gaaataaaag attattttga
     5821 atatggtgac ttgggaattt tacttgggca aacaataaaa aattgtgaat accttaaaaa
     5881 agtaaatgta gttaaatcta tggaaacaat aagagccaca gtggttgggg caggttctca
     5941 tactactgaa gtaagtggaa gtacaataac ttacactagc gaaaagtttc cacttaagaa
     6001 cttgccagtt ttaaaattat cagttgatga agaaacaggt acttcaaaac aaatcagtga
     6061 agctataaaa aataaactta attggtttaa actagaaaat gatttgcaga aagttgctat
     6121 agccatgata ggaaagaaaa atccaacctt tttagatata caagagtatg ctaaagggtt
     6181 tgtagatgga atgcaacaaa tcatagaaaa tgatttacct tttatagtaa ttgttgaaaa
     6241 tgatatggca aaagtgttag gtcaagcaat atatgcatta ttaaattatg aaaaagaaat
     6301 tgtttgtata gacagtgtca aggttgaaaa tggagactat atcgacattg gaaaacctat
     6361 agttgaaggt aaagttttac ctgtggtaat aaaaacatta gtttttaatt aatagaagga
     6421 ggtaagtaaa tgattttaaa tacaaaatta tttggacgta cttatcagtt taaaggctta
     6481 tatgatgtaa tggctaaagc taatgaagaa aaatcaggag ataaattagc aggtcttgca
     6541 gcagaatcag ctgaagaaag aatagcagca aaagttgttt tatcacacat aaaattggag
     6601 gatataagaa ataatcctgc agtggcatat gaagaagatg aagtaacaag aataatacaa
     6661 gatgctgtaa atgaaaaagt ttatgaagaa ataaaaaatt ggacagtatc tgaacttaga
     6721 gaatggctat tggattcaaa tactacaagt cttgatataa gaagaatatc aagaggatta
     6781 acttctgaaa tgatagcagc agttgcaaaa ttaatgtcaa atatggactt agtttatgca
     6841 gctaaaaaga taaaggttac tgctcactgt aatacaacta taggagaaca aggaacatta
     6901 tcagcaagac ttcaacctaa tcatccaaca gacgacccag atggaatact tgcatcactt
     6961 cttgaaggtt taacttttgg tataggagat gcagtacttg gattaaatcc agttgatgac
     7021 tctgttgaga gcgttactaa agtattaaaa agatttgaag aagtaaaaca aaaatatcag
     7081 ataccaactc aaacatgtgt attggcacac gttactacac aaatggaagc aataagaaat
     7141 ggtgctcctg cagatttaat attccaatct atagcaggtt ctgaaaaagg aaatgaagca
     7201 tttggattta atggcgacac tatagaagaa gctagacagt tagctttaaa acacggtaca
     7261 gctacaggac ctaacgttat gtattttgaa actggacaag gttcagaatt atcttcagaa
     7321 gcacatcatg gaatagacca agttactatg gaagctagat gctatggatt tgctaaaaag
     7381 tatgaaccat tcttagtaaa tacagttgtt gggtttatag gacctgagta cttatatgat
     7441 gctagagaag ttgtaagagc aggacttgaa gaccacttta tgggtaaact acatggctta
     7501 ccaatgggtg ttgatgtttg ttacactaac catatgaagg ctgaccaaaa tgatatggaa
     7561 aacttagcca tactattaac aacagcagga tgtacgtatt ttatgggaat acctcatgga
     7621 gatgatgtta tgcttaacta tcaaacaaca ggataccatg aaacagcatc acttagagaa
     7681 atgtttggac taacagcaat aaaagaattt gatgcttgga tggaaaagat gggattctat
     7741 aaagatggta agttaacaga gttggcggga gacgcatctg tattattagg ataggaggta
     7801 tttaatatga atgagaagga tttaaaagca ttggtagaac aattagttgg acaaatggtt
     7861 ggagaattag atactaatgt tgtatctgag actgttaaaa agacaactga agttgtagta
     7921 gacaacaatg cttttataga tgatataaca gaagttgata taagaaagca attactagta
     7981 aaaaatccta aggatgcaga agcttattta gatatgaaag caaaaacacc tgcaagatta
     8041 ggtataggaa gagctggtac tagatataaa acagaaacag ttttaagatt tagagcagac
     8101 catgctgctg cacaagatgc tgtattctct tatgtagatg aagaatttat aaaagaaaat
     8161 aatatgtttg cagtggaaac tttatgtaaa gataaagatg aatacttaac aagaccagat
     8221 ttgggaagaa aattttctca agaaactata aataatataa aatctaaatt tggtacaaat
     8281 caaaaggttt taatattagt tggagatggg cttagctcag ctgcaataga ggcaaattta
     8341 aaagactgtg tgccagctat aaaacagggt ctaaaaatgt atggtataga ttcaagtgaa
     8401 attttatttg ttaaacattg tagagttggt gctatggacc acttaggtga agaattaggt
     8461 tgtgaagtta tatgtatgtt ggttggagaa agaccaggat tagttactgc tgaatcaatg
     8521 tcagcatata tagcttataa gccatatata ggaatggcag aagcaaaaag aacagttata
     8581 tcaaatattc acaaaggtgg aacaactgca gttgaagcag gtgctcatat agctgaactt
     8641 ataaaaacta tgttagataa aaaagcttca ggaatagacc ttaaataaag tcgagggagt
     8701 gtagaatatg aaaaatgatg taattcgtcc taatatacta ggaattaaaa taatttcgaa
     8761 tataagtcca gaaatggcac aaaaactaga attaaaatca catcacaaaa gtttaggttt
     8821 aataactgca gactgtgatg atgttacgta tacagctctt gacgaagcaa ctaaagcttc
     8881 agaggttgat gttgtatatg caaaatcaat gtatgcaggt gctgcaaatg cttcaacaaa
     8941 acttgctggt gaagtattag gaataatagc aggcccaagc ccagcagaag taagaagtgg
     9001 tctaaatgca gtagttgatt ttctagagta tggagcaact tttataagtg caaatgatga
     9061 tgattcaata gcatactatg ctcactgcgt atcaagaaca ggtacatacc tttcagagat
     9121 agcaggaata agagaaggtg aagcattagc ttatttagta gcaccaccac ttgaagcaat
     9181 gtatgcacta gatgcagcaa tgaaagctgc agatgttaag atgtgtgaat tatttgcacc
     9241 tccaactgaa acaaactttg gtggagcatt acttacaggt tctcaatcgg cttgtaaagc
     9301 agcatgtgat gcatttgcag aagcagtaaa atcagttgca gacaatccaa caggatttta
     9361 aggatagtta gatggatgct ataggcttaa tagaagtaat aggatatgtt gctgcaatag
     9421 aagcttctga tgcttgttta aaatctgcaa atgttaatat tgtaagaatt gataaggttg
     9481 gggcaggtat tgttacttta acaatatgtg gagatgtcgg tgctgtaaaa gctgctttag
     9541 aagcaggtga aatagcagct tccagagttg gtacattaag aacatcacat attataccga
     9601 gaatccataa tgaagtaaca aatgctttat tcaaaactaa agaacctaaa gtctgtgaag
     9661 tttcagaaga taaagaaaaa aactttgaga ttacattgga aataactgat aatgtatcga
     9721 aaaatttatc agaaaaagaa aatgtaattt tagatgtcaa tgaaaacagt ggagatactg
     9781 aaaatttata tagtgatata gataaaacaa acaaaaaaat tgaaacagtt gatgatagtt
     9841 taacagaagc taatgaggaa aaaatagaaa atactaatga taaagaagta ttggaacaag
     9901 ttaaaataga aaattccgat tttgatagag atgacctatc aaagaacagt gtaaaaaaac
     9961 ttaaagctat ggctaagaaa ttagattctt caataactta taaagattta aatgttctta
    10021 aaaaagaaga attaataaac ttagttaaaa agcttagcag aagggataga tagttatgac
    10081 acttttagat aaagatttag tatctataca agaagtcaga actcttgtaa gaagatctaa
    10141 agtggctcaa gagaaacttt ctaaaatgag tcaagagcaa atagataaaa tagtaaaagc
    10201 tgttgtagat gcagcattta ataatgctac taaattagct aaaatggcaa atgaagaaac
    10261 tggatttgga aaatgggaag ataaagtaat taaaaatact tttgcatcta tgggagttta
    10321 tgaaacttta aagaacacaa aaactattgg aataattggt gaagataaag aagccaatac
    10381 gatagatata gctacaccag ttggagttgt ggcaggtctt ataccttcaa caaacccaac
    10441 atcaacagta atatacaaaa ctataatatc tctaaaagca ggaaatacta tagttttctc
    10501 tccacatcca aatgcaaaaa actgcatact tgaaacagtt aatataataa gtgaggctgc
    10561 tataaaggct ggagctccag aggattcaat attatgtata actaatccaa gtatagttgg
    10621 aaccaatgaa ttgatgaaac ataaagatac aaacttaata ctagcaactg gaggagaagc
    10681 aatggttaga gctgcttact cttcaggaac accagctatt ggagttggac caggaaatgg
    10741 accagcattt atagataaat cagcaaatgt taaattagca atcaaaagaa taatagattc
    10801 taaaactttt gataatggtg ttatatgtgc atcagagcaa tctatagtaa tagaaaaatc
    10861 tatggaagaa actgttttaa aagagcttaa aaatcaaggt gcatatgttt taaatgaaga
    10921 ggaaagtgaa agattagcaa aattcatatt aagagctaat ggtactatga atccacaaat
    10981 agttggtaaa agtgttgagg taatagcaaa tttagcagac ttaacaaata tacctaaaaa
    11041 tacaaaagtt ctagttgcaa gagaaaataa agttggtcac aatgtagcct attcaagaga
    11101 aaaacttaca ccaatactag cgttttatgt tgaagaaaat gtagaagcta ttatgaacag
    11161 atgtagagaa atattgttaa atgaaggtgc aggtcataca ttctcaatgc atgtaaatga
    11221 tgaagaattg gtaaaaagat ttgcccttaa tatgccagta tcaagaatac ttgtgaatac
    11281 tccttcagca cttggaggaa taggtgcatc aacaaacctt gtaccagcac taacacttgg
    11341 ttgtggtgca ataggtggaa gttcaacttc aaacaatata ggaccacttg atttaataaa
    11401 tgttaaaaga gtagcttatg gaatcaaaga aatagaagaa cttggaagag ataaatctca
    11461 agttgaaaaa tcaatgtttg actcaatgga taaagaagaa ttaataaatc ttcttgttca
    11521 aaaactagtc aaagaactag cataactaaa ataaacaatg aaacttatgg ctatcgctat
    11581 aaatttataa aaatataaac tataaaaaac tataaaatta aatttttgga ggataagatt
    11641 atggcaagtg caaacgcatt aggaatgata gaaactaaag gattagtagg agcaatagaa
    11701 gcagcagatg caatggttaa agctgcaaat gtacaattag taggaaaaga gcaagtaggt
    11761 ggaggattag ttactgtaat ggtaagagga gatgttggag ctgttaaagc tgctacagat
    11821 gcaggagctg cagctgccga gagagttgga gaactaatat cagttcatgt tataccaaga
    11881 ccacactttg aagtagatgc tatattacca aaggtatcag ctgagtaata taagaataat
    11941 tttaaaattt aaattttgat tgaaaaagag gctatctcaa aaatgagtct tactaataaa
    12001 ctcaatgtat tttgaggtag tcttttttat acatctaata ggagatgtga taaaatgggt
    12061 gttttaacag aaaacgaagt aaaaagaaaa attggaaaaa acaaaaataa tataactgaa
    12121 ttttatatag aaaaaggtca aataataact ccttctgcaa aatcttattt agctgagaga
    12181 agtattgagc ttaaatatgt tgataaactt tcagatgtaa acaatgaacc taaagaagtt
    12241 gtaaaagaag tagttaaaga gaaaaaatat aaatatacta ctgtatttgg cgctaaattg
    12301 gatgaaaaac cagaacatat gactcaatta aatggaaatc ttttagtttt taaagaccat
    12361 aaaagaataa tattgagagg aaaaatagat tctcttgaat cagaaatact aaaggtacag
    12421 atactttgtc agaaactaga tatgccaaag cttgttaagg aattgcaaga agtattagac
    12481 tttgtaagaa atataatgag atgtgaagtt ttagatgaaa agctagaaga ctttaaactt
    12541 ttaggattaa ctcctcaaga acttagagaa caatctcact atcctaaaaa atactttggt
    12601 ataggacatg aattcccaag ttatgaaatg ggagaagtag tagtagctat aaatagtata
    12661 agaagtaata cacgagaggt tgaacttgca gcctatgaag catttaaggg tgagtatgga
    12721 aatgttcaca gagaagattt aataaaggca cttaatagat tatcgtcagt attttggata
    12781 atgatatata aggttagaac taataagtat aagtagttgg aggacataat ggagaatcta
    12841 ttaaatgaaa tagtggaaga agtaataact agagttaaaa aagaagcatt tatagaagtt
    12901 gaagcatcag gaagacacgt tcacttaagt agagaagatg tcgataaatt atttggagaa
    12961 ggatatactc ttacaaaact aaaagaccta tctcaaccag gtcaatatgc atgtaaggaa
    13021 agagttacaa taactggtcc taaaggaagt ataaaaaatg tagttgtact tggaccatgt
    13081 agaaatgaaa ctcaggtaga aatatcttta acagatggtt caactcttgg actaaaagct
    13141 cctataaaac aaagtggaga cttagaaggt actttaagta ttaaaatatc aacacaatat
    13201 ggtgaagttg aacttgataa ggggttaatg gttgctaaaa gacatataca catgactcct
    13261 aaagatgctg aaaaattcaa tgtatgtgat aaagagatag ttgaagccaa ggtgatgggt
    13321 caaagacctt taacatttga tgatgtagta ataagagtga gtgacagttt taaaacttat
    13381 atgcatatag actatgatga agccaatgca tgtggttatt caaaaggaac tgttgctaaa
    13441 ataataaaga aagcataaat ttaacctact atataaatta cctctaggga ggatttaata
    13501 tgaattatga taaccttgta gatttaataa cggaagaaat ctataaaaaa ataaatagta
    13561 atgaaataaa aatatctaat aaacctaaag ctgtgattgt atttgaacaa gataacaata
    13621 aatttaactt attaaaagat gaatttgaaa tactagaatt tgataaaagt ataagggaat
    13681 gtgaaatagt gatagtttca agactttgta tgagggggct atcgaatata gcacttggaa
    13741 attcaacaag tgatgaagaa agatttatac taaaaatgat tatgaaaggc aaaaaagtat
    13801 atgtcctaga tgaaggaatt gaatacaaaa aatataagga tactgcacca aaagcattat
    13861 ataagaaatt tatgtcattt gaagatgaaa tatgtaaatt tggtgtagaa attataaaag
    13921 acttaaatgt tataactaaa aataagttaa atataaagaa agaactagaa agtactagag
    13981 gttctaaaaa agaagataca aaagttttag ataaagatgt tttaaattta accagtgtag
    14041 aaagtgtaaa aatagataat gaatttagtt tggatttaag gaataaaaaa cttatatcgg
    14101 aagctgactt aagaaaacca actataaatg gagttaaaaa catattagtt aataaaaaaa
    14161 gtataataac accattagct gtagatttta tgagaataca ccatctcaaa ctaaagaaac
    14221 tgtaaggagg taattttatg gatataggaa aagttgtagg taatgtttgg gctacaagaa
    14281 aagatgaaaa attatgtggc caaaaacttt tagttgtaaa aattttaaaa actaaagaca
    14341 catacaagga aggcttcatg gttgcagccg ataatgcagg agcaggaaat ggagacttag
    14401 ttcttatatc caaaggttcc tcagctagag agtcgattga aaaatctcat gttccagtag
    14461 atgcaactat agttggagta gtcgattcgt ttgaggtgtt tgatgaataa aagtatagga
    14521 gctatagagt ttaaaagcat tgctaaaggt atagaagtat ccaatgaaat gataaagaaa
    14581 tcttcagttg atgtattgta cttaaaaagt atttgcccag gtaaatttct aataatagta
    14641 ggtggagaaa cttcttatat aaatgaatgt gtagattatg gaataaaact tggagaagga
    14701 tatattgtag ataacttcgt aataaatgca atttcacaag aaatattaga tggatttaaa
    14761 aataagtatc aaaagctaga tagtatagtc tctataggcg ttgttgaaag tagtaaagta
    14821 tgcgctggaa taaaaatgct tgataagact ttaaaatctg gcgacttagt attagtaaaa
    14881 cttcaattat cttttgctat aggtggcaaa ttagtatatg tagtagcagg agatttaagt
    14941 agtttagaat atgcattaaa agaaagtgaa aatgtagtaa gagaaaaaga agtaatatat
    15001 aagactgtaa taccatcagt agacagtcaa ataataaaaa gtctaataaa ataatggggg
    15061 gaattatgag tataaacgaa attataatct atgtaatggt agtcttcatg gtacttggag
    15121 ctatagataa atgtataggt aacaaatttg gtcttggaga acaatttgaa gaaggtataa
    15181 tggcaatggg gtcacttgca gttgcaatgg ttggtgttat atgtttagca ccagttcttg
    15241 ctgatgtatt aagaccaatt gtagtaccag tatttaatat acttggagct gacccagcta
    15301 tgtttgctgg aagtttatta gctaatgata tgggtggtgc gccacttgca cttgaacttg
    15361 cgaaagaccc taattctggg ttatttggtg gattaatagt aggggctatg atgggaccta
    15421 caatagtatt tataatacca gttgcgcttg gaataattga aaaagaagac caaaaattct
    15481 tagcaacagg aatacttgca ggtataataa caatacctat tggagcattc gttggtggat
    15541 tagttgcagg attcgaggta atgatggttc ttagaaactt agtgccaata ataatatttg
    15601 cagtagttat agcacttgga ttattaaaat tcgaaaatgc tatgataaaa ggattcacat
    15661 actttggaaa aggtgtagta atagttataa ctttaggact tgctgctgcc atagttgaag
    15721 ctttaacagg aatagttgta atacctggaa tgaaaccaat cagtgaaggt atagaaatag
    15781 ttggagatat agcaatagtt ttagcaggag cattcccatt agtgtttgtt ataacaaagg
    15841 tatttaataa accattaatg gctttaggaa aagttcttgg aatgaatgat gtatcagcag
    15901 caggccttgt tgcaagttta gctaactgta taccaatgtt tggtatgatg aaagatatgg
    15961 acaatagagg aaaaataata aatgtagcat tctctgtatc agcagcattc gtatttggag
    16021 accacttagg atttacggca ggatttaatt ctaatatgat aacaccaatg atagttgcta
    16081 aattagttgg tggtataaca gcagtattgt tagctatggt tatcgcaaat aagacactaa
    16141 ggaaggagaa tgtataagat ggatatatca aatatagata aaaatttaat tgaaacatta
    16201 gtaagacaaa taatagaaga aaaaataagt ggaactaaag acacagtaga ttttgttaga
    16261 aataaagaca ttagtggaat aacttctata aagttaccaa ctgtaaaagt tagtgaatct
    16321 gatagattag atacagggaa tccaagtgat gtagtttaca caaaagattt atttacatta
    16381 gaggaaagcc caagattagg ttgtggtatg atggaaatga aagaaacaac ttttgattgg
    16441 actctaaact atgatgaaat agattatgta atagatggaa ctttagatat aataatagat
    16501 ggaagaaagg tttctgcttc ttcaggtgaa ttaatattca taccaaaagg aagtaagata
    16561 caattttctg tacctgatta tgctagattt atatatgtaa catatccggc agattgggca
    16621 tctcaaaatt aataaaaatc aagttaagtt aataataatc aaataaataa tttaaaatac
    16681 aaactcttaa ataaacacac atatttaaaa aagctatgaa ttaaaattca tagctttttt
    16741 tataaagaat gttatatttt agacgtattt tttctat
//
LOCUS       DAMJWP010000064        16691 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.066, whole genome shotgun sequence.
ACCESSION   DAMJWP010000064 DAMJWP010000000
VERSION     DAMJWP010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16691
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.066"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(26..1102)
                     /locus_tag="PC700_001771"
     CDS             complement(26..1102)
                     /locus_tag="PC700_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDF2581083.1"
                     /translation="MSKKLFIPGPIDVKEEVLQKMATPMIGHRGKDASMLQKSISEKM
                     QKLFYTNNTILLSTSSGTGLMEGSIRSCTSKKAAVFSCGSFGDRWYKMAVANNVPADI
                     FKVELGEATTPEMVDKVLSTGEYDLITVTHNETSTGIRNPIEEIGEVVKKYEDVIYCV
                     DTVSSAGGIKVEVDKIGIDICITSVQKALGLPPGMSICTFSQKAIDRAKQVPFRGVYL
                     DLLAMYEYLIKKNYQYPSTPSLSHMFALDFQLDNILDEGLDNRFNRHEDMANLVRNWA
                     KKHFQIFTNENHLSNTLTVIENTQGISVSNLNSKLQERGFQIANGYGDLKEKTFRISH
                     MGDYTVEDVQELLDNIDDILGFNK"
     gene            complement(1475..1990)
                     /locus_tag="PC700_001772"
     CDS             complement(1475..1990)
                     /locus_tag="PC700_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HDF2581084.1"
                     /translation="MDFLAKYKVLKKQNSSKSCLVCGTQNELGLKADFYELENGELVS
                     ICNTKDWHQSYPGRVHGGMSAAILDETIGRAVSINDDQIWGVTVSLELKYKKPVPTDA
                     TIKVVGRITKENRKLFEGTGEIILPNGDIAVTATGKYMKMPIGQIAEGDFSNEEWFFE
                     ESKEKVEYIEL"
     gene            complement(2353..3423)
                     /gene="leuB"
                     /locus_tag="PC700_001773"
     CDS             complement(2353..3423)
                     /gene="leuB"
                     /locus_tag="PC700_001773"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HDF2581085.1"
                     /translation="MNCNIAVIKGDGVGPEIIDEGIKVLNKICCKFNHRFDCEYVLAG
                     GCAIDEIGEPLPNKTVETCRKNEAVLLGAVGGPKWDKCKGDKRPESGLLKLRESLGLF
                     ANLRPATMYESIKEASPLRTDIVEKGIDFVVVRELTGGIYFGERGRKIIDGIENAYDV
                     EIYNENEIRRIGKRAFEIARNRNKKLISVDKANVLESSRLWRSIMEDLAKEFEDVELS
                     HMYVDNAAMQVVKDPSQFDVIVTNNIFGDIISDEASMITGSIGMLPSASLREDSFGMY
                     EPIHGSAPDIAGKDIVNPIATILSVSMMLRHSFNLEEEAKCIEDAVQSVLNKGYRTID
                     IYNGVGNVVGTRAMGELIVNEI"
     gene            complement(3430..3921)
                     /gene="leuD"
                     /locus_tag="PC700_001774"
     CDS             complement(3430..3921)
                     /gene="leuD"
                     /locus_tag="PC700_001774"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HDF2581086.1"
                     /translation="MIANGSVFKFGDNIDTDVIIPARYLNIADYKELATHCMEDIDDK
                     FISKVKKGDIIVATKNFGCGSSREHAPIVIKESGVSCVIASTFARIFFRNSINIGLPI
                     LECEEAANNIDEGDNIEVDFSTGVIKNITKEKEYKAEPFPEFMQNIILNEGLINSIKA
                     NRG"
     gene            complement(3936..5213)
                     /gene="leuC"
                     /locus_tag="PC700_001775"
     CDS             complement(3936..5213)
                     /gene="leuC"
                     /locus_tag="PC700_001775"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HDF2581087.1"
                     /translation="MGMTMTQKILAKHAKLNEVKKGQLIEADLDVVLGNDITSPVAIR
                     EFEKLGIEDVYDKTKVVMVLDHFTPNKDIKSAEQCKFTRSFAKSKGVVNFFDVGDMGI
                     EHVLLPEKGIVTAGDVIIGADSHTCTYGALGAFSTGVGSTDMGAGMATGKCWFKVPGA
                     IKFVLKNKPNKWISGKDIILHIIGEIGVDGALYKSMEFCGDGVEYLSMDDRFTICNMA
                     IEAGAKNGIFPVDDKTMEYINSHKCSTITKDVNIYEADEDAVYDEVYEIDLAELKETV
                     AFPHLPENTRTVDEIDKDIKIDQVVIGSCTNGRISDLEVVAEIMKGKKVADGVRVMIF
                     PGTQKVYLEAIEKGYITTFIEAGAAVSTPTCGPCLGGHMGILAAGEKSISTTNRNFVG
                     RMGHVDSEVYLASPAVAAASAITGKISKPSEII"
     gene            complement(5170..6912)
                     /gene="leuA"
                     /locus_tag="PC700_001776"
     CDS             complement(5170..6912)
                     /gene="leuA"
                     /locus_tag="PC700_001776"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDF2581088.1"
                     /translation="MKCGKYKKYDKMQIVNRKWPDNEIFKAPIWCSVDLRDGNQSLPT
                     PMSVNEKVRMFKMLIDTGFKEIEVGFPSASNTEYTFLRKLIDENMIPDDVTIQVLTQS
                     RAHLIEKTFESIKGCKKAIIHLYNSTSVLQRDVVFNMSKQEIIDIAVEGAKLFNEEVK
                     KYPETEFTFEYSPESFTGTEMDYALEICEAVIDVWKPTPQKKVIINLPSTVEMATPNV
                     YADQIEWFCKNISCRDSIILSLHTHNDRGTCTAASELGLLAGADRLEGTLFGNGERTG
                     NMDIVNVGLNLYTQGIDPELDFSNIDKIIGIYEDCTKLMVHDRHPYAGNLVHCAFSGS
                     HQDAIRKGMIAMKNRDNDYWEVPYLPIDPHDIGREYKEIIRINSQSGKGGAVYIMETD
                     YGFMIPKNMHSDFGNVVKMESDRIGEELSSEAIFNLFKKEYIEVESPYKVKKYKIKSM
                     DELNYENDDSNDTNMIEMTARISYMGNEQRIVGIGNGPVDSFNNALKQCGMKDYKFRY
                     YWEHALEEGSHSRGVAYVGIEHNNEVYFGVSISENINTAAINALMNAINKSYIEEEIK
                     NGDDYDAENISQTC"
     misc_binding    complement(7036..7282)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(7430..7876)
                     /locus_tag="PC700_001777"
     CDS             complement(7430..7876)
                     /locus_tag="PC700_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581089.1"
                     /translation="MSKNKFIYMNYYDNGYFKKGMTIYMVLALIILATIFIYSTTILY
                     ILCIITAVFLFKQGRDFSNKYEGKVYITLDNHSILINNQCIFSIKSKQSQKFNYKIIE
                     KIEVVKNILNIYTDESNYKIRLRALSLEDEKKLLNIIDEKMKKFKA"
     gene            complement(7897..8616)
                     /locus_tag="PC700_001778"
     CDS             complement(7897..8616)
                     /locus_tag="PC700_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HDF2581090.1"
                     /translation="MKKSIMDVHCHTLISGHAHSTFKENVEEASKKNIKYLGISDHGP
                     NMPGGPHPFYFYNLHLLPREVQGVKILRGIEGNIMDYSGNLDVQEDMLQHLDYIIASL
                     HRPCIASGTKEENTNAILKVMDKPKVKIIGHPDDSRYPLDYEPIVKKAKDKNILLEIN
                     NSSLSSNSHRTGTWENVSHMLTLCKTYGTRVILGTDSHICYSIGEFENAEKVLEAVDF
                     PDELVINYHEDEIIEFFDINF"
     gene            complement(8734..10008)
                     /locus_tag="PC700_001779"
     CDS             complement(8734..10008)
                     /locus_tag="PC700_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HDF2581091.1"
                     /translation="MFIMENDLTMQELLDQQEQVFSKVKVGELTTGKITAVRNDEVQL
                     GLDYGFDGIIPISELNIEKNQYIEDIYHIGDEITAVITKVSQKDGTITLSKLQLDKRN
                     DFAELQKAYDEHRIITVNVEKNIDKGVFANYNTYTFFIPISQLDTKFITDTSKFVGLN
                     LEVYIKELDVRKNRLVASHRDVLQERINKEREERRAQIKAEKEAERARIKQEREEEKA
                     RIKAAKEDLFNSLEVGQKRDGKVTKIMPYGAFVDIGGIEGLAHINNLAWTRVESVEDV
                     VSEGQEVEVYVLDVNKETKKIALALKDINNDPWDLIAKEVQIDDVVNAKVLRIIEKGA
                     FVQIKEGVDAYLPISELSDTRVAKVTNVVNIGDEVKVKILDFKPKTKRMLVSIKEATR
                     EPEEDITEYLEVEESLGSIGELFKDKFKDLEV"
     gene            complement(10208..11575)
                     /locus_tag="PC700_001780"
     CDS             complement(10208..11575)
                     /locus_tag="PC700_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2581092.1"
                     /translation="MENSNTNLGSESVGKLLFKLATPAIIAQIVNVLYNIVDRIFIGR
                     MENGEVAMAGVGVAFPIIIIITACSYLIGMGGGPLAAIKMGEQNNDEAEKIMSNSFSV
                     LVILAILLTIGFKIGKEPLLWMFGASESTIRYSMDYLNIYLIGTVFVQISMGMNTFIN
                     TQGFATTGMMTVAIGALINIILDPIFIFGFNMGVKGAALATIIAQGVSAIWVLMFLFG
                     KKSILKIKKKYMIPKASIILPVLGLGISPFIMQSTESLVLIALNSKLQMYGGDLAVGS
                     MAIMSSIMQILMLPNMGVTQGAQPIISYNYGSGQLDRVKKTFKLCLLSCFTYSTILWL
                     LLMIFPAFFVSIFNKNPQLLSMTSWSIKIYFAGAFMFGIQIACQQTFLALGKATISLV
                     LALLRKIVLLIPLIFILPTFFNEKLFAVILAEPVADITAATITAISFFIFYKCFLSKP
                     KAIKE"
     gene            complement(11825..12289)
                     /locus_tag="PC700_001781"
     CDS             complement(11825..12289)
                     /locus_tag="PC700_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HDF2581093.1"
                     /translation="MSLRIGGNSLGYLHILILSMVPVTELRGAIPIGIAMGLNPIWVY
                     VFSVIGSTLVSIPLILTFRHVLQFLRGKKLFKGIADVIDRKINSRMKKLKSVSIIGII
                     LFVGIPLPTTGTWTASAIASILKMRIKDAFMGVFLGNLLSGVIVSALSLHII"
     gene            complement(12294..12923)
                     /locus_tag="PC700_001782"
     CDS             complement(12294..12923)
                     /locus_tag="PC700_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HDF2581094.1"
                     /translation="MKKSVLLLDVDYTVINTDSMIDFFIYSLKNKTFKTIIKLPYIIF
                     ILFMYLIRMIPLKKAKEAIFYPIVDFSEEDLKKFFDDCIMKKINESMKKVIYKNKEED
                     NVIIMITASPYAYMKYFKYYGLADKVIGTEFFYENSRYKNQFIGENCKGIEKVKRIKA
                     VLGKLGIEIDYENSYAYSDSKSDLPMLSLTKNAFLVSKKDGEVIEQINS"
     gene            complement(12957..13844)
                     /locus_tag="PC700_001783"
     CDS             complement(12957..13844)
                     /locus_tag="PC700_001783"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HDF2581095.1"
                     /translation="MKQTNTSIRLLTKISDYIKLMRIKQYIKNFFVFSAIIFSNNILN
                     INLFLNTFIAFVCFCLMSSSVYALNDAIDMDKDKKHPKKCNRPVASGRISKKSANILF
                     VVLALISVCLSTVVSFNLSIILSLYLINNILYSLKLKNIILLDVFSISLGFILRVYAG
                     CVAISVSLSNWIILCTFFLSLYLALGKRKKEIETLRDDAVEHRKILEDYDIENLNQMM
                     IVILSSTIVCYSLYSTSNPEKPHMIFTTIFVVYGVLRYNYIINTTNENNPTDIVLKDN
                     ALKINVILWIITCLIILIF"
     gene            complement(13873..15111)
                     /locus_tag="PC700_001784"
     CDS             complement(13873..15111)
                     /locus_tag="PC700_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581096.1"
                     /translation="MDGDILMSKILKYKNEMFLFILVVITYLIITKIFFSKTTIFYNL
                     NNTYDVLLDTDTGVLFNLNVFAISQDNSKHILFSAIISIFAYPIYLFCTSIANPGTTD
                     FNSAYGFGLICLQIITSAMSITLVFNHIKKIKMQRLTLILLTMIMIFSFPQLFMTLNV
                     ERFIYSQFSLIFFIVIANKMKGKNSYLVELAAIPLFGITISNIYLYFFNMIFEFKLKI
                     GKMLKHLITFILMAYICVVSTKSYESFMNLGNVIQYDTKFISGEPILEKIAMIIERLL
                     YSVFYFPGASIKKGLFLQNGEVATIPVILTLLALCFCVLSVIENSEKRVPKLCMGIII
                     FNLTLHGIVGYNLVNSSIMAINFSFAVIILLAYFTKALRKNEKNMYNIFLSLLLVTIV
                     ISNVNGFIEILNIGIKSYPV"
     gene            complement(15495..16637)
                     /locus_tag="PC700_001785"
     CDS             complement(15495..16637)
                     /locus_tag="PC700_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDF2581097.1"
                     /translation="MRYVEYGKTGKMVSVVGYGGLRFDLEKSNQENADLVKYAYEKGI
                     NYFDTAPGYCDDRSEDIFGLAFRQMIKEGKTDFYVSTKEKPKACDTSEKAIEAVKKSI
                     ERLGVSKINFYHVWNIRKMEHYELAMRPGGQYEGLLKCKEEGLIDHIVFSSHQPGDEV
                     IDILSENKFEGVTMGINILNFPYRLKGAKYAVDNGYGVVAMNPLSGGTIPKYNKELSF
                     LSKDGESTIESALRFNIGISQISISLIGFNKKQDIDDACKIADENRIYSDEDIHDIET
                     RLNKNMNEICTGCGYCKVCPKGINTPAYMLFYNEKQMFKKSDEEMTKLVYGLGHWNYT
                     MNSKAKAKECISCGKCEVECTQHLPIIDRLKEIKKWEEDGANSVKV"
BASE COUNT         5410 a         3041 c         1740 g         6500 t
ORIGIN      
        1 ttaaaacata catattttta caatattatt tattaaaacc taatatatca tcaatattat
       61 ctaataattc ttgtacatct tcgacagtat agtcacccat atgtgaaatt ctaaaagttt
      121 tttcttttaa atctccatag ccatttgcta tttggaatcc tcgttcttgt aattttgaat
      181 ttaaatttga tacactaata ccttgagtat tttctataac agtaagtgta ttggataagt
      241 gattttcatt ggtaaaaatt tggaagtgtt ttttagccca gtttctaact aagtttgcca
      301 tgtcttcatg tctgttgaat ctgttatcta ggccttcatc taatatatta tctaattgga
      361 aatctaaagc gaacatgtga gaaagagatg gtgttgaagg gtattggtag tttttcttga
      421 ttaagtattc atacattgct aacaaatcaa gatatactcc tctaaaaggt acttgcttag
      481 ctctatcgat tgctttttga gaaaatgtgc aaatagacat tcctggtggt aatcccagtg
      541 ctttttgtac tgatgttata caaatatcta ttccaatctt atcaacctcg acttttattc
      601 caccggctga acttacagta tctacacagt agattacatc ttcatacttt ttaacaactt
      661 ctccaatttc ttctattgga tttcttattc cagttgaagt ttcattgtga gttacagtta
      721 ttaaatcgta ttctccggta gaaagcacct tatcaaccat ttctggcgtt gttgcctctc
      781 ctaattcaac tttaaatata tccgctggga cattattagc cactgccatt ttgtaccatc
      841 tatcaccaaa ggatccacag gaaaatactg ctgccttttt ggatgtacag gaacgaatgg
      901 agccttccat taaaccagtt ccggatgacg tggataaaag tatcgtgttg tttgtataga
      961 atagtttttg catcttttca cttatactct tttggagcat agatgcatct tttcctctat
     1021 gtcctatcat tggtgtagcc atcttttgca agacttcttc tttaacatca ataggaccag
     1081 gaataaataa tttcttactc attataattc cccccttttt agtttgtaat atggatagcg
     1141 tttttactgt ccttattttt aataactata atatcaagct gaattaacta tgtcaacata
     1201 aaatttattt tttatatata atttatttta tatattttat gaaaacattc attttctgcc
     1261 ttttcataag tctgaatttt ctgaattatt ctctcaaaaa aaatatactt tttatctata
     1321 taaattaatt tttttagtat gtaaatttga cgctattacg ataaatcttt ttagtataac
     1381 taatttattt ttatcttgta tttttattgt atttttgaat tttatttttt ctaaatataa
     1441 aatttatgtt ttaaaatata aaatttttat ttaatcaaag ttcaatatat tccacttttt
     1501 ctttcgattc ttcaaaaaac cattcttcat tagaaaaatc accttctgca atttgtccaa
     1561 taggcatttt catatacttt ccagttgctg tcactgctat atctccattt ggtagaatta
     1621 tttctcctgt accttcaaat aattttctgt tttctttagt tattcttcca acaactttta
     1681 tagtagcatc tgttggtact ggtttcttat atttcaactc taaagatact gttactcccc
     1741 atatttggtc atcatttata cttacagctc ttccaatagt ctcatctaat attgcagcag
     1801 acatacctcc atgaactctc ccaggataac tttgatgcca atcctttgta ttacaaattg
     1861 atacaagctc tccattttca agttcataaa aatctgcttt aagtcctagc tcattttgtg
     1921 ttccacaaac taaacaagat ttactactat tttgcttctt caaaacttta tactttgcca
     1981 aaaaatccat ctcctaaccg acattagtag tcgcttaata ttaaataatt ctaaaatata
     2041 ttatataata ttaataaaat ccctgcaaac ttctaataat aaaaataggc tacttcaata
     2101 tgataagttt aatttaaagt attattttcc aaaaaataac tttatcaaaa tagaaactca
     2161 aagtagatat aagcctcttt ttcgctatta aaatagtaaa aatatatttg acttaatata
     2221 aaaaagagtg tctcattaac taataaagtt tattttgaga cacccctcat aaaacaaatt
     2281 atactacaat agatttttac taattctaca agttatatat ttaaattata ttattcaaat
     2341 taaactataa ttctaaattt catttactat taattcgccc attgctcttg ttcctacaac
     2401 atttcccaca ccattataga tatcaatagt tctataacct ttattgagta cactttgtac
     2461 agcatcttca atacacttag cttcttcttc taagttaaaa ctatgtctta acatcatgct
     2521 tacagataga atagttgcaa ttggatttac aatatctttt ccagctatat caggcgcact
     2581 tccatgtatt ggttcataca taccaaaact atcttctcta agtgatgctg atggtaacat
     2641 acctatagaa cccgtaatca tagatgcttc atcagatatt atatctccaa atatattatt
     2701 agtcactatt acatcaaatt ggctagggtc ttttacaact tgcatagcag cattatcaac
     2761 atacatatgt gacagttcta catcctcaaa ctctttagct aagtcttcca ttatacttct
     2821 ccaaagtctt gaactctcaa gcacatttgc cttatctaca cttataagtt ttttatttct
     2881 atttctggct atttcaaaag ctctcttacc aattcttctt atttcatttt cgttatatat
     2941 ttctacatca taagcatttt cgataccatc aatgattttt cttccacgtt ctccaaaata
     3001 aatgccccct gttaattctc ttacaacaac aaaatcaatg cctttctcaa ctatatccgt
     3061 tctaagtggt gaagcttctt ttatactttc atacattgta gctggtctta aatttgcaaa
     3121 taaacccaat gactctctta attttaaaag accagactct ggtcttttat ctcccttaca
     3181 tttatcccac tttggtcctc caacagcacc aagcaataca gcttcgtttt ttcgacaagt
     3241 ttcaacagtt ttattaggta gaggttctcc aatttcatca atcgcgcatc ctccagctaa
     3301 tacatattca caatcgaatc tatgattaaa tttacaacaa attttattta gaacctttat
     3361 accttcatct ataatttccg gtccaacacc atcacctttt atgactgcta tattacaatt
     3421 catactccac taccccctat ttgcttttat tgagttaatt aagccttcat ttaatataat
     3481 attttgcata aactcaggaa atggttctgc tttatattct ttttcttttg ttatattttt
     3541 tatcacacct gtagaaaaat ctacttctat attatcccct tcatctatgt tattggcagc
     3601 ttcttcacat tcaagtatag gaagccctat atttatagag tttctaaaaa atattctagc
     3661 aaaagtagaa gcaatcacac aggatactcc actttctttt attacaattg gtgcatgttc
     3721 tcttgatgaa ccacaaccaa agtttttagt tgcaactata atatctcctt tttttacctt
     3781 agaaataaat ttatcatcaa tatcctccat acaatgcgtt gctagttctt tataatcagc
     3841 tatatttaaa tatctagctg gtattattac atctgtatct atattgtcac cgaatttaaa
     3901 aacacttcca ttggcaatca taaattttac ctcccttaaa ttatttctga tggttttgat
     3961 atttttccag ttatagctga tgctgctgct acagctgggc ttgcaagata cacctctgaa
     4021 tcaacatgcc ccattcttcc tacgaaattc ctatttgttg ttgaaatgga tttttctcct
     4081 gctgcaagta tacccatatg tccccctaga catggaccac aagttggtgt agaaactgct
     4141 gctcctgcct ctataaaagt ggtaatatac cctttttcta tagcttcaag atatactttt
     4201 tgagttccag gaaatatcat aactctaact ccatctgcta cttttttgcc tttcattatc
     4261 tcagctacta cttcaaggtc tgatattctt ccatttgtac aagaacctat tacaacttgg
     4321 tctatcttta tatccttatc tatctcatct actgttctag tattttcagg aagatgagga
     4381 aaggctactg tttcttttag ttcggctaaa tctatttcgt atacttcatc atagactgca
     4441 tcttcatctg cttcatatat atttacatct tttgttatag ttgaacactt atgtgaattt
     4501 atatattcca tagtcttatc gtctactgga aagattccat tttttgctcc tgcttctatt
     4561 gccatattac aaatagtaaa tctatcatcc atagataagt attctactcc atctccacaa
     4621 aattccatcg acttgtaaag tgctccatca acacctatct cccctattat atgaagtatt
     4681 atatctttac cacttatcca tttatttggt ttattcttca aaacaaattt tattgctcca
     4741 ggaaccttaa accaacattt tcctgtagcc atacctgctc ccatatcagt tgaacctaca
     4801 ccagtagaaa atgctcctaa tgctccatat gtgcaagtat gagagtcagc acctataatt
     4861 acatctccag cagttactat acctttttca ggaagaagaa catgctctat tcccatatct
     4921 cctacatcaa aaaaatttac aactcctttt gattttgcga aacttcttgt aaatttacat
     4981 tgttctgcac tctttatatc tttattaggt gtaaagtggt ctaaaaccat cactacttta
     5041 gtcttatcat aaacatcttc aattcctaat ttttcaaatt ctctaatggc tactggtgat
     5101 gttatatcat tacctaaaac tacatctaaa tctgcttcga ttaattgccc tttcttaact
     5161 tcatttaatt tagcatgttt ggctaatatt ttctgcgtca tagtcatccc cattttttat
     5221 ttcctcctca atataacttt tatttattgc attcataagt gcattaatag ctgctgtatt
     5281 tatgttttcc gatatactta caccaaaata gacttcatta ttgtgctcta ttcctacata
     5341 agctacccct cttgaatgtg acccttcctc taatgcatgt tcccagtaat atctaaattt
     5401 ataatctttc atgccacatt gctttagtgc attattaaat gaatctacag gtccatttcc
     5461 tataccaaca atcctttgtt catttcccat ataacttatt cttgcagtca tctcaatcat
     5521 gttagtatca ttagaatcat cattttcata gtttaattca tccattgatt ttattttata
     5581 cttcttaact ttataagggc tctcaacttc aatatattct ttcttaaata aattaaatat
     5641 tgcttcacta ctaagttctt ccccaattct atcagattcc attttgacta catttccaaa
     5701 atcagaatgc atattcttag gtatcataaa tccataatct gtttccatta tataaacagc
     5761 tcctcctttt ccagattggc tatttatcct tataatttct ttgtattctc ttccaatatc
     5821 atgagggtct attggtaaat atggaacttc ccagtaatca ttatctctgt ttttcattgc
     5881 tatcatgcct tttctaatag catcttgatg tgaacctgaa aaagcacaat gtacaagatt
     5941 acctgcatat ggatgtctat catgtaccat aagttttgta cagtcttcat atataccaat
     6001 tatcttatct atattgctaa aatctagctc tgggtctatt ccttgtgtat aaagattaag
     6061 tcccacattt actatatcca tatttccagt tctttcacca tttccaaata gtgttccttc
     6121 aagtctatcg gcaccagcca ataatccaag ttcacttgca gccgtacaag ttcctctatc
     6181 attgtgagta tgcaaactta gtataataga atccctacaa cttatatttt tacaaaacca
     6241 ttctatttgg tctgcatata cgtttggtgt tgccatttcc actgttgatg gaagatttat
     6301 tattactttt ttttgaggag ttggtttcca aacatctatc actgcctcac atatttcaag
     6361 tgcataatcc atctctgttc ctgtaaaact ctctggtgaa tattcaaaag taaattctgt
     6421 ttctggatat ttttttactt cttcattaaa taattttgct ccttcaactg ctatatctat
     6481 tatttcttgt ttactcatat taaaaaccac atctctttga agaacagatg tagagttata
     6541 taaatgtata attgcttttt tacatccctt tattgattca aatgtttttt ctataagatg
     6601 agccctagat tgtgtaagta cttgtatagt tacatcatct ggaatcatat tctcatctat
     6661 taattttctt aaaaatgtat actctgtatt tgatgctgat ggaaatccta cctctatttc
     6721 tttaaatcct gtatcaataa gcattttgaa cattctgact ttttcattta cactcatagg
     6781 cgttggcagt gactgatttc cgtctcttaa atcgacacta caccaaattg gagccttaaa
     6841 tatttcatta tctggccatt tacgatttac tatttgcatt ttatcatatt ttttatactt
     6901 tccacacttc atgttatttc cccctttttc tgttttttgt attcagtata ccttatatcg
     6961 acgcaatcct gcacacttca taaggataaa tgtcaatttt atcattagaa taaaaaaaac
     7021 ctcatctcta ataaaattag agacgaggtt ttgctcgcgg taccactcta tttagctttc
     7081 taaaaagctc ccttagtaat agccaataca ataattataa tgactatatt tcctataacg
     7141 tggaattact ccgattattc taagatttaa atcttagaaa acagctccta gacaagttca
     7201 gaactatcaa ctactaaatc acaccatcct ttagctctct gaaagtttca ttgttcttac
     7261 tacttctatt catcactttt ttaatcattt aaattttcta aatattttga tttaatagtt
     7321 attatataca cattaaaatg tattgtcaac aaaaaaaata attatctcaa ttttttattt
     7381 ttgataagca attctataaa acatgctaac tattgaaaac agtatgatat tatgccttaa
     7441 actttttcat cttttcatct atgatattca ataatttttt ttcatcttcc aaactcaatg
     7501 cccttaatct aattttataa ttagattcgt cagtgtatat atttaatata ttttttacta
     7561 cttctatttt ttctattatc ttataattaa acttttgact ttgtttagat tttatactga
     7621 atatacattg attatttatt aatattgaat gattatcgag agtaatatat acttttccct
     7681 catatttatt tgaaaaatca cgaccttgtt taaatagaaa aactgctgtt attatacata
     7741 aaatatataa tattgtcgtt gaatatataa aaattgtagc taaaattata agtgctaaaa
     7801 ccatatatat agtcattcct tttttaaaat aaccattgtc ataataattc atatagataa
     7861 acttattttt actcaaattt tttcactccc aagattctaa aaatttatat caaaaaattc
     7921 tataatttca tcttcatgat aatttatgac taattcatct ggaaaatcta ctgcttctaa
     7981 aactttttct gcattttcaa attctcctat agaataacat atatgagagt cagtaccaag
     8041 aattactctt gtaccatatg ttttacacaa tgtcaacata tgacttacat tttcccaagt
     8101 acccgttcta tgagagtttg aacttaatga tgaattattt atttctaaaa ggatattttt
     8161 atctttagct ttttttacta taggttcata atctaaagga tacctactgt catctggatg
     8221 tccaattatt tttaccttag gtttatccat aacttttaaa atagcatttg tattttcttc
     8281 ttttgtacca gaagctatac aaggtctatg caaacttgcg attatataat ccaaatgttg
     8341 caacatatct tcttgaacat ctaaattacc actataatcc attatatttc cttctattcc
     8401 cctaagaatc tttacacctt gcacttcacg tggaagcaaa tgcaaattat aaaaatagaa
     8461 tggatgtggt cctcctggca tattaggtcc gtggtctgat attcctaagt attttatatt
     8521 cttcttactc gcctcttcga cattttcctt gaaagtacta tgtgcatgtc cacttattag
     8581 agtgtgacaa tgtacatcca taatagactt tttcattaaa tttcctccta atattttaca
     8641 taaattcgtg aaaatagcta aagggctatt tcaaaactaa agctgtcata atagacagtg
     8701 taacgtttta aaacagcccc attttcaatt attttacact tctaaatctt taaatttatc
     8761 tttaaataat tctccaatac ttcctaaaga ttcctctact tctaggtatt ctgttatatc
     8821 ctcttctggc tctctagttg cttcttttat tgaaaccaac attcttttag tctttggttt
     8881 aaaatcaaga atcttaactt taacctcatc tccaatatta acaacattag taactttagc
     8941 cactctagta tcactcaatt cagatattgg taaatacgca tctacacctt ctttaatttg
     9001 aacaaaagcc cctttttcta ttattctaag aactttagca ttgacaacat catcaatttg
     9061 tacttctttt gctattaaat cccaagggtc attatttatg tcttttaatg ccaaggcaat
     9121 ttttttagtc tctttattta catccaacac ataaacttct acttcttgtc cctctgaaac
     9181 aacatcttca acactttcaa ctcttgtcca agctaaatta tttatatgtg ctaatccttc
     9241 aatcccacca atatccacaa atgcaccata aggcattatc tttgtcacct taccatctct
     9301 tttttgtcca acttcaagag aattaaataa gtcttcttta gctgctttga tacgagcttt
     9361 ttcttcttct ctttcttgct taattcttgc tctttcagct tctttttctg ccttaatttg
     9421 agctcttctt tcttctctct ctttatttat acgctcttgt aatacatctc tatgagatgc
     9481 tacaagtcta ttttttctaa catccaattc ttttatataa acttctaagt taagaccaac
     9541 aaatttagat gtatctgtaa taaattttgt atcaagttga gaaattggta taaaaaatgt
     9601 atatgtattg taattagcaa atacaccttt atctatattc ttttcaacat ttacagttat
     9661 tattctatgt tcatcataag ccttttgtaa ctcggcaaaa tcatttcttt tgtcaagttg
     9721 taatttagat aaagttattg taccatcttt ttgagatact tttgttatta cagcagttat
     9781 ttcatcacct atgtggtata tatcttctat atattgattt ttttctatgt tcaattcact
     9841 tataggtatt attccatcaa acccatagtc taatcctaat tgtacttcat catttctcac
     9901 tgcagttatt tttccagttg tcaattctcc aactttcact ttgctgaata cttgttcttg
     9961 ctggtcaagt aattcttgca ttgttaaatc attttccatt ataaacatcc tttcctctta
    10021 ttgattatca tatctgtaaa tattatacca ttaatattgc aaatacttac atttttctta
    10081 ctataatttt taaaataaat tcaacttcat atatttatac attatagaaa atatcttatt
    10141 aaattaaaat acaaattcta atttttatct aataaaatat tttcataaaa aatttatatt
    10201 gtatatctta ttcttttatt gctttaggtt ttgatagaaa acatttataa aatataaaga
    10261 aagaaattgc tgttatagta gcagctgtta tatctgcaac tggctctgct aaaattactg
    10321 caaaaagttt ttcattaaag aatgttggga gtataaatat taaaggtatt aaaagtacaa
    10381 tctttctaag tagagctaaa accaaagata ttgttgcttt tccaagtgct aaaaatgtct
    10441 gttgacaagc tatttgaatc ccaaacataa aagcaccagc aaaatatatt tttatactcc
    10501 aactagtcat actaagtaac tgcggattct tattaaatat actaacaaaa aatgcaggaa
    10561 atatcattaa taaaagccat aaaatagtag aatatgtaaa acatgacaat aaacacaact
    10621 taaatgtctt ttttactcta tcaagttgac ctgaaccata attataactt attattggtt
    10681 gtgctccttg tgtaaccccc atattaggaa gcataagaat ttgcattata ctgctcatta
    10741 tggccattga acctactgct aaatctcctc catacatttg taatttacta tttaaggcta
    10801 taagtactaa actttctgta gattgcatta taaatggaga gattcccaag ccaagcactg
    10861 gtaagatgat acttgctttt ggtatcatat acttcttttt tattttaagt atacttttct
    10921 ttccaaagag aaacataagt acccaaatag cagaaacgcc ttgtgcaatt atagtagcta
    10981 atgcagctcc ttttacaccc atattaaagc caaatataaa aatcgggtca agtattatgt
    11041 ttattaaagc acctatagca actgtcatca ttccagttgt agcaaaccct tgggtattta
    11101 taaaagtatt catacccatt gaaatctgaa caaacactgt acctattaaa taaatattta
    11161 aatagtccat tgaatatcta attgtagatt cacttgctcc aaacatccaa agtaaaggct
    11221 cttttccaat cttaaaacct atagtcaaaa gtattgctaa aatcaccagt acagaaaaac
    11281 tgttactcat tattttctct gcctcatcat tgttttgctc acccattttt atagcagcta
    11341 atggacctcc tcccataccg attaaataac tacatgcagt tattataatt attattggaa
    11401 aggccactcc aacaccagcc atagcaactt ctccattttc cattctacca ataaaaatcc
    11461 tatcgactat gttgtataaa acatttacaa tttgtgcaat aatagcaggt gtagcaagtt
    11521 taaataataa tttacccaca ctttcactgc ctaagttagt gtttgaattt tccattttaa
    11581 aacctcctca atccaataat taaaatgtat cactaaaatt ttattctata taaaaatttc
    11641 attttatcag cagacaaatt tagagtactt tccctacaag taatactata ctatatttct
    11701 tttgccatag taaacatttt agaactaact gaatttaaat actttatctc attttttatt
    11761 tattattaaa aatttttcac atgtactcat aaaaaaacta tccaagtata ttacttagat
    11821 agttttatat tatatgtaaa gaaagagctg aaactataac ccctgataac aaattgccaa
    11881 gaaatactcc cataaaagca tcttttattc tcatttttaa tatggaagct atagcagatg
    11941 cagtccatgt tcctgtagtt ggcagtggta tacctacaaa tagtattata ccaattatcg
    12001 aaacactttt taatttcttc attcttgagt ttatttttct atcaattaca tcagcaatcc
    12061 ctttaaatag tttcttccct cttaaaaatt gtaatacatg tctgaatgtc aatatcaagg
    12121 gaatcgatac caatgtagaa cctattacac taaatacata aacccatata ggatttaagc
    12181 ccattgcaat acctatagga atggctcccc tcaattctgt tacaggaacc atagataaaa
    12241 ttaatatgtg taaatatccc aaactattcc ctcctattct aagtgacatt aagttatgaa
    12301 tttatttgct ctataacctc accatccttt ttactaacta aaaatgcatt tttagtcaat
    12361 gaaagcattg gtaaatcact cttagagtct gaatatgcat atgaattttc ataatcaatc
    12421 tctatcccaa gtttacctaa tacagccttt attcttttta ctttttcaat tcccttacag
    12481 ttttctccaa tgaattgatt tttatatctt gaattttcat aaaaaaactc cgtgcctata
    12541 accttgtctg caagaccata atacttaaaa tatttcatat atgcatatgg agatgcagtt
    12601 atcataatta taacattatc ttcttcttta tttttgtaaa tgaccttttt catagattcg
    12661 ttaatttttt tcattatgca atcatcaaaa aactttttta aatcttcttc actaaaatct
    12721 actattggat aaaagattgc ctcttttgcc ttcttcaagg gtatcattct aattaaatac
    12781 ataaatagaa taaaaataat gtatggcaac tttataatag ttttaaaggt tttatttttt
    12841 aaagagtaaa taaaaaaatc tatcatgcta tcagtattta taacagtata atctacatca
    12901 agtaataata cacttttttt cataagtatt tccccatcct atcactaaaa atatcactaa
    12961 aaaatcaata ttattaaaca tgtaattatc cataagatta cgtttatttt taaagcatta
    13021 tcttttagga caatatcagt aggattattt tcatttgtag tgttaattat ataattatat
    13081 ctaagtacac cataaactac aaatattgtt gtaaatatca tatgtggctt ttctggatta
    13141 cttgtagaat acaatgaata acatacaatt gtagaactta atataactat catcatttga
    13201 tttagatttt ctatgtcata atcttccaat atttttctat gttcaactgc atcatctctt
    13261 agagtttcta tttctttttt acgttttcca agagctaagt acagagataa gaaaaatgta
    13321 cataaaatta tccaatttga taaactaaca cttatggcta cacatcctgc ataaacccta
    13381 agtataaaac caagagatat tgaaaacaca tctaatagta ttatattctt tagtttaaga
    13441 gaatataata tattgtttat caaatagaga ctaagaatta tagaaagatt aaaactaaca
    13501 actgttgata aacagactga aattaaagct aatactacaa atagtatatt agcacttttt
    13561 ttgcttattc ttccacttgc cactggtcta ttacattttt taggatgttt tttatcttta
    13621 tccatgtcta tagcatcatt cagtgcatat actgatgatg acattaggca aaaacacaca
    13681 aacgcaataa acgtatttaa aaataaattt atatttagta tgttgtttga aaaaattata
    13741 gctgaaaata caaagaaatt ctttatatat tgctttattc tcattaactt tatataatct
    13801 gaaattttag taagaagcct tatacttgta ttggtctgtt tcataaactt actacccttt
    13861 caatattatt ttttaaacag gatatgattt tatccctatg tttaaaattt ctataaatcc
    13921 attgacatta ctgattacta tagtaaccaa taacagcgat agaaatatat tatacatatt
    13981 cttttcattt tttctaagcg ctttcgtaaa gtaagccaat aaaattataa ctgcaaaact
    14041 aaaatttata gccataatac ttgaattgac tagattatat cctactatac catgtaaagt
    14101 tagattgaat ataattattc ccatacatag cttaggaact cttttttctg aattttcaat
    14161 aactgataaa acacaaaaac ataaagctaa taatgtaaga attactggta ttgtagcaac
    14221 ttctccattt tgcaaaaata accctttttt aatacttgct ccaggaaaat aaaaaaccga
    14281 atacaataat ctctctataa tcattgcaat cttttccaaa attggttctc cacttataaa
    14341 tttcgtatca tactgtatta catttccaag gttcataaaa ctctcataag acttcgttga
    14401 aactacacaa atatatgcca ttaggataaa tgtaattaaa tgttttaaca tttttcctat
    14461 ttttaattta aactcaaata tcatattaaa aaaatacaaa tatatattac taatagttat
    14521 tccaaataga ggaatagctg ctaattctac taaataagaa ttttttcctt tcatcttatt
    14581 tgcaataact atgaaaaata ttaatgaaaa ttggctatat ataaatctct ctacatttaa
    14641 agtcataaat aactgtggaa aagaaaatat cattatcata gtaagtaaaa ttaaagttaa
    14701 tctctgcatt tttatctttt taatatgatt aaagactaaa gttattgaca tagcactagt
    14761 tattatttgt agacaaatta gtccaaaccc ataggcacta ttaaaatctg ttgtcccagg
    14821 atttgctatt gaagtacaaa ataggtatat tggatatgca aaaattgata taattgctga
    14881 aaacaatatg tgcttactat tgtcttgact tatagcaaat acatttaaat tgaataaaac
    14941 ccctgtatct gtatctaaca atacatcata agtattgttt aaattataaa atatagtagt
    15001 cttagaaaaa aatatcttag ttattattaa gtaagttatt acaactaata taaacaaaaa
    15061 catctcgttt ttatatttta atattttact catcaatata tcaccatcca ttcattatat
    15121 aggatttgct gacacctaca atttattcct tatatttaat agccatcttc ataatgttct
    15181 tacttgtttt attgtaattg aataactctt tatttacaac cacaatattt actttattga
    15241 tgttataatc agtcaagtat tgtttatata acatagatta gtgttgtata aaatgagaat
    15301 ttgtaagcaa tattaatgta aaatatttga aaaactttat ataaaaatat acaatgttgc
    15361 ttatggaatt aaactataac aaattaaagc ataatttaag gagacaaaat tataatctgt
    15421 ctccttaaat attaaaacta tattttaaaa cattactaaa tactatacaa atttcaaata
    15481 tagtataaaa ttaattagac tttaacacta ttagctccat cttcttccca ttttttaatt
    15541 tcttttaatc tatctattat aggtaaatgc tgagtacatt caacttcaca ttttccacaa
    15601 gatatacatt ctttagcttt tgccttacta ttcattgtgt aattccaatg tcctaaacca
    15661 taaactaact ttgtcatttc ttcatcactc tttttaaaca tctgcttttc attataaaaa
    15721 agcatatagg ctggggtatt aatacctttt ggacatactt tacaataacc acaacctgta
    15781 catatttcgt tcatattctt atttaatcta gtctcaatat catgtatatc ctcatctgaa
    15841 taaattctat tttcgtctgc aattttacat gcatcatcta tatcttgttt tttattaaac
    15901 cctatcaaag atatacttat ttgtgaaatt cctatgttga atcttaaagc tgattctata
    15961 gtactttcgc catcttttga tagaaaagaa agttctttat tatactttgg aatagttcca
    16021 ccacttaatg gattcattgc cacaacacca tatccattat caacagcata tttggcacct
    16081 tttagcctat atggaaagtt taatatattt attcccatag ttacaccttc aaacttattt
    16141 tcacttagaa tatctataac ctcatctcct ggttgatgag atgaaaatac tatatggtct
    16201 atcaatcctt cttctttaca tttcaataat ccttcatatt gacctccagg tctcattgct
    16261 agttcgtaat gttccatctt tctaatattc caaacatgat aaaaatttat tttagatacg
    16321 cctaatcgtt ctattgattt ttttacagcc tctatagctt tttctgatgt atcacatgct
    16381 tttggctttt ctttagttga tacataaaaa tcggtcttac cttcctttat catttgtcta
    16441 aaggctagtc caaatatatc ttcacttctg tcatcacaat accctggcgc tgtatcaaag
    16501 taattaatac ctttttcata cgcatatttg actaaatcag cattttcttg attacttttt
    16561 tctaaatcaa accttaatcc tccatagcca actactgaaa ccattttccc tgtctttcca
    16621 tattcaacgt atctcattgt aaattcctcc ttagatatat taagacttaa tttctttata
    16681 gaaaatatta t
//
LOCUS       DAMJWP010000065        16683 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.067, whole genome shotgun sequence.
ACCESSION   DAMJWP010000065 DAMJWP010000000
VERSION     DAMJWP010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16683
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.067"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(72..275)
                     /locus_tag="PC700_001786"
                     /pseudo
     CDS             complement(72..275)
                     /locus_tag="PC700_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
     gene            complement(275..604)
                     /locus_tag="PC700_001787"
     CDS             complement(275..604)
                     /locus_tag="PC700_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1904 domain-containing protein"
                     /protein_id="HDF2581098.1"
                     /translation="MPQIKIRGINENDICKISKKMINDLVEAVKCPRDYFEIECIKSV
                     AIRDGKIADVYPFVEVAWFDRGQEVQDIVARIITDSIRNNLDVESMDLAFTVFEKEKY
                     YENGEHF"
     gene            934..1860
                     /gene="fba"
                     /locus_tag="PC700_001788"
     CDS             934..1860
                     /gene="fba"
                     /locus_tag="PC700_001788"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HDF2581099.1"
                     /translation="MALITTKEMFKKAYEGGFAIGAFNISDLEQLQGVLKAAKAKNSY
                     VMIQASMSAVKYAGPHTLVEMVKAASDEIGVDVALHLDHGPNMDAIKTCIDAGFSSVM
                     IDGSHFDFEENVRITKEAVEYAHSKGVVVEAELGVLAGTEDDVTSDVHKYTQPAEAVE
                     FVERTGVDSLAIAIGTSHGAFKFKGEAKLRFDILEEIQSKLPGFPIVLHGASAVDQNA
                     VATCNEFGGNIAGAKGVPVDMLRKASSMAVCKINMDTDLRLAMTAAIRKFLAENPKEF
                     DPRKYIGAGRDAIQAVVESKIDDVLGSANSIN"
     gene            complement(1995..3626)
                     /locus_tag="PC700_001789"
     CDS             complement(1995..3626)
                     /locus_tag="PC700_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2581100.1"
                     /translation="MDNNLIGLIEHVENPAILCKESGEIIYCNHLIDSIFSFLDIKKP
                     RNINELDSNFDKTEILTDSKKKIAFRELRMTAHIYNMKDNNNENNIVYLFEKSLISDK
                     VIEDIIEHIDEVVVVFNKDGVIEKMNTVSDEILPFKRTEVLGRNITDLVRQGLVEEPI
                     ILNMLKVKKKIYRNIVYPDGKLIAYTAVPRWDSKGKLTGGVLTGRDISRVIKLESQIK
                     YSDISEDTEYISQSKIMDNIKKVVKRAAASDSSIFINGESGVGKEIIARTIYKYSSRR
                     DKPFIAINCGAIPNELLESEFFGYEEGSFTGAKKKGKKGLFEEANGGTIFLDEIGELP
                     MQMQKKLLRVIQENTITRIGGSKPIKIDVRYISATNISHEDLRNNLKFRQDLYYRLSV
                     IPVKIPPLRERKEDIVPLVNYFLKLYNEKYNREVEVSPKVIELLEEYSWPGNIRELKN
                     IIERFVVLSAKNVIGEDEFNMLINLDMIDNETDDLSPIVVNGIMNLNDAYKIVDQIMI
                     SKAINKYGSITKAAEVIGIYPSTIHRKIKSGHIHV"
     gene            complement(4109..5146)
                     /locus_tag="PC700_001790"
     CDS             complement(4109..5146)
                     /locus_tag="PC700_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDF2581101.1"
                     /translation="MNDIKDLSSYKNVWIFAEQREGKIAPVVIELLGEGRKLAKEVDA
                     ELCAILLGKDVDGLAKELITFGADKVYVADDALLEKYTTDAYTKVIKDAIDEIKPEIM
                     LFGATHIGRDLAPRIASRVGTGLTADCTKLEIDPEDKKIKQTRPAFGGNIMATIICPN
                     HRPQMSTVRPGVMDKAEKDETRTGEVIALDYKITQDDIRTTVLETVKTKKDLVSLTDA
                     NVIVSGGLGLGGPEGFEMLKKLADKLGGVVGSSRAAVDAGWIDHSHQVGQTGTTVKPN
                     LYIACGISGAIQHLAGMQSSDFIIAINKNPAAPILEIADYGVVGDLHEIVPMLIEKLD
                     SVDDLLEAIKA"
     gene            complement(5165..5950)
                     /locus_tag="PC700_001791"
     CDS             complement(5165..5950)
                     /locus_tag="PC700_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDF2581102.1"
                     /translation="MKILVCVKQVPDTNEVRINKETGTLIRDGVPSILNPDDANALEE
                     ALKIKDEHDDVTITVITMGPPQADFMLRECLAMGADDAILLSDRAFGGADTWATSNTI
                     AAGISKVGDYDIIFAGRQAIDGDTAQVGPQIAEKLDIPQVTYVQDFKIEGKDIIVQRQ
                     LEDGYELIKVSTPVLLTAVKELNTPRYMSVDKIVKAYKHDVKVWTIDDLDVNKEEVGL
                     KASPTKVFRSFTPEPRGKGEILEGKADEIASKIIIGLKQKHII"
     gene            complement(5983..7116)
                     /locus_tag="PC700_001792"
     CDS             complement(5983..7116)
                     /locus_tag="PC700_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HDF2581103.1"
                     /translation="MLYNKEQELLRKAVRDFVSKELDTLPAEMDKTGVMPKELIKKLA
                     DAKFISSNIPEEYGGGGAGYVSYAIVMEEIARRCASTATFVTAGSSLASLPILYNGTE
                     EQKQKYLKGIATGELIGAFGLTEPGAGSDAGGQQTTAELVGDHYILNGRKTFITNGPF
                     CDVAIVIAVTDRSKGLRGTSAFIVESKWDGFSTGAHEDKMGIRGTETSDLIFENVKVP
                     KENLLGKEGQGFKIAMGTLEVGRIGVAALALGIAQGALDEAVKYTKQRVQFGKPIAKF
                     QNTQFTIADMETKVCAARGLVYDAAQKRDAGMRVAQESAMAKYYASEIANEVAYKALQ
                     LHGGYGFIKDYEIERMYRDARIVSIYEGTSEVQKMVISSNVLK"
     gene            complement(7164..8291)
                     /gene="hadC"
                     /locus_tag="PC700_001793"
     CDS             complement(7164..8291)
                     /gene="hadC"
                     /locus_tag="PC700_001793"
                     /EC_number="4.2.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     beta"
                     /protein_id="HDF2581104.1"
                     /translation="MEAILSKMKEVVENPNAAVKKYKNETGKKAIGCFPVYCPEEIIH
                     AAGMLPVGIWGGQTELDLAKQYFPAFACSIMQSCLEYGLKGAYDELSGVIIPGMCDTL
                     ICLGQNWKSAVPHIKYISLVHPQNRKLEAGVKYLISEYKGVKRELEEICGYEIEEAKI
                     HESIEVYNEHRKTMRDFVEVAYKHSNTIKPSIRSLVIKSGFFMRKEEHTELVKDLIAK
                     LNAMPEEVCSGKKVLLTGILADSKDILDILEDNNISVVADDLAQETRQFRTDVPAGDD
                     ALERLARQWSNIEGCSLAYDPKKKRGSLIVDEVKKKDIDGVIFCMMKFCDPEEYDYPL
                     VRKDIEDSGIPTLYVEIDQQTQNNEQARTRIQTFAEMMSLA"
     gene            complement(8291..9517)
                     /gene="hadB"
                     /locus_tag="PC700_001794"
     CDS             complement(8291..9517)
                     /gene="hadB"
                     /locus_tag="PC700_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     HadB"
                     /protein_id="HDF2581105.1"
                     /translation="MSEKKEARVVINDLLAEQYANAFKAKEEGRPVGWSTSVFPQELA
                     EVFDLNVLYPENQAAGVAAKKGSLELCEIAESKGYSIDLCAYARTNFGLLENGGCEAL
                     DMPAPDFLLCCNNICNQVIKWYENISRELDIPLIMIDTTFNNEDEVTQSRIDYIKAQF
                     EEAIKQLEIISGKKFDPKKFEEVMKISAENGRLWKYSMSLPADSSPSPMNGFDLFTYM
                     AVIVCARGKKETTEAFKLLIEELEDNMKTGKSSFRGEEKYRIMMEGIPCWPYIGYKMK
                     TLAKFGVNMTGSVYPHAWALQYEVNDLDGMAVAYSTMFNNVNLDRMTKYRVDSLVEGK
                     CDGAFYHMNRSCKLMSLIQYEMQRRAAEETGLPYAGFDGDQADPRAFTNAQFETRIQG
                     LVEVMEERKKLNRGEI"
     gene            complement(9532..10332)
                     /gene="hadI"
                     /locus_tag="PC700_001795"
     CDS             complement(9532..10332)
                     /gene="hadI"
                     /locus_tag="PC700_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyisocaproyl-CoA dehydratase activator
                     HadI"
                     /protein_id="HDF2581106.1"
                     /translation="MYTMGLDIGSTASKGVILKNGEDIVASETISSGTGTTGPSRVLE
                     KLYGKTGLAREDIKKVVVTGYGRMNYSDADKQISELSCHARGVNFIIPETRTIIDIGG
                     QDAKVLKLDNNGRLLNFLMNDKCAAGTGRFLDVMAKIIEVDVSELGSISMNSQNEVSI
                     SSTCTVFAESEVISHLSENAKIEDIVAGIHTSVAKRVSSLVKRIGVQRNVVMVGGVAR
                     NSGIVRAMAREINTEIIVPDIPQLTGALGAALYAFDEAKESQKEVKNI"
     gene            complement(10371..11570)
                     /gene="hadA"
                     /locus_tag="PC700_001796"
     CDS             complement(10371..11570)
                     /gene="hadA"
                     /locus_tag="PC700_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocaprenoyl-CoA:2-hydroxyisocaproate
                     CoA-transferase HadA"
                     /protein_id="HDF2581107.1"
                     /translation="MLLEGVKVVELSSFIAAPCCAKMLGDWGAEVIKIEPIEGDGIRV
                     MGGTFKSPASDDENPMFELENGNKKGVSINVKSKEGVEILHKLLSEADIFVTNVRVQA
                     LEKMGIAYDQIKDKYPGLIFSQILGYGEKGPLKDKPGFDYTAYFARGGVSQSVMEKGT
                     SPANTAAGFGDHYAGLALAAGSLAALHKKAQTGKGERVTVSLFHTAIYGMGTMITTAQ
                     YGNEMPLSRENPNSPLMTTYKCKDGRWIQLALIQYNKWLGKFCKVINREYILEDDRYN
                     NIDSMVNHVEDLVKIVGEAMLEKTLDEWSALLEEADLPFEKIQSCEDLLDDEQAWAND
                     FLFKKTYDSGNTGVLVNTPVMFRNEGIKEYTPAPKVGQHTVEVLKSLGYDEEKINNFK
                     DSKVVRY"
     gene            12247..13245
                     /locus_tag="PC700_001797"
     CDS             12247..13245
                     /locus_tag="PC700_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HDF2581108.1"
                     /translation="MKILVFGARDYEEPVIKKWSEEHKDVQVDIYPENMTEENVVKAK
                     GYDGISIQQTNYIDNPYIYETLKDAGVKVIASRTAGVDMIHFDLVNENGLIVTNVPSY
                     SPNAIAELAVTQAMNLLRKTPLVKKKVCEGDYRWIAELLGTEVRSITVGVIGTGKIGA
                     TSAKLFKGLGANVIAFDQYPNSDLNDILTYKDSLEDLLKEADLITLHTPLLEGTKHMI
                     NKDTLAIMKDGAYIVNTGRGGLINTGDLIEALESGKIRAAALDTFETEGLFLNKKMNP
                     GELTDPEINKLLSMEQVIFTHHLGFFTSTAIENIVYSSLSSAVEVIKTGTATNRVN"
     gene            13607..14134
                     /locus_tag="PC700_001798"
     CDS             13607..14134
                     /locus_tag="PC700_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04002 family protein"
                     /protein_id="HDF2581109.1"
                     /translation="MEKMNKVTSKKTTYIVTSALFASIICLTIAYILHIPVGGNNGYV
                     HIGDAFIYLAATILPTNYAIAASAIGAGLADLSTGAAIWVIPTIIIKPILVLFFTSKS
                     DKIINKRNIVASVVAGIVGLVLYMFAEGIIIGSFTSAFVMSLLGLLQPIGSFIVFIIL
                     GMALDKLDFKKRYFN"
     gene            14161..14757
                     /locus_tag="PC700_001799"
     CDS             14161..14757
                     /locus_tag="PC700_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04100 family radical SAM protein"
                     /protein_id="HDF2581110.1"
                     /translation="MNILYTIENSIYVNITNTCPCSCVFCIRNEKDEVANSGSLWLEH
                     EPSVDEVKEAFNKYNLDDYDEIVFCGYGEPLMRINELIEVAKFIKEKSSIKIRINTNG
                     LSDLIHNKKTAILLKDVIDTVSISLNAPNKEAYNRVTQPKFGEKSFDYMLGFARDCKK
                     YIKEVAFSVVDEISPEEIEESKQLAKKLDIPLRVRHKN"
     gene            complement(14822..16405)
                     /gene="asnB"
                     /locus_tag="PC700_001800"
     CDS             complement(14822..16405)
                     /gene="asnB"
                     /locus_tag="PC700_001800"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDF2581111.1"
                     /translation="MINLEEKYLTRKDTYMIKILEYNGHIRNWKVLCEELGIDSSLCR
                     DERERAILVEAYKTWGYDMANHMHGMFAFALWDTVEKKLFCLRDQFGVKPFYYYETEN
                     GKLLYGSSIRKIMEQPGFVKELNEDMLQIYMSFTYVAGENTFFRGVKKLMPGHYLVYQ
                     NKTVCIGRYWKPEFHPDHSKSIEEWTDEIHTTLQKIMPEVKSENETAELFLSGGVDSS
                     YILAMSDIEKTGSCGYEEERFDESKLAQQTANILGCKNSRYIITPEEYFASVPYVMYH
                     MEQPTGDASAIVFAIGCKAAAENTNICYSGEGADEFFGGYNIYHKAECYGENLKTFYI
                     GNTNIMNEGEKRKILKKYNPDVLPIEVVKGIYEETEELDTLTNMSNVDIQIWLEGDIY
                     FNIDKMSTAVGLEVRMPLTDTRIFDIASRIPSEYKVKGKENKIAFRTAASKTLPEEIA
                     FRNKLGFIVPIRIWMADDQYNKDIRDKFNSEIADKFFNIDEINEIFNDYISGNSDNWR
                     KIWMIYTFLVWYEIYFVKC"
BASE COUNT         5159 a         2972 c         2109 g         6443 t
ORIGIN      
        1 gtttccctat atgctgacta ttttagctac taactcatga ctaaagtcac gagtgtacgt
       61 agctattttt taatcaaata tatatattct ctatatataa cttcttctcc attaaataat
      121 atctcatcat tttcagtgtc tacttgagtt cctggatttg aaacttcact tccatttact
      181 actacagacc cttgtttaca atacttcttt atttcacttc tacttccata acctaaattt
      241 gaaagaattt tatctatacg ttgcttttta gccattaaaa atgctctcca ttctcataat
      301 atttttcttt ttcaaatact gtaaatgcta aatccatact ttctacatct aaattatttc
      361 ttatactatc agttattatt cttgctacta tatcttgtac ttcttgtcct ctatcaaacc
      421 aagctacttc aacaaaagga tatacatctg ctattttacc atctcttatt gcaactgact
      481 ttatacattc tatttcaaag tagtctcttg gacattttac agcttcaact aaatcattta
      541 tcattttttt acttatttta catatatcat tttcattaat tcctcttatt tttatctgtg
      601 gcatagttta tctcctttat tttaaatttt tagtgaatta tcatccactt aattcccttc
      661 gataacgttt gaagtggtaa cttcttggtc aagacaacta ttgttatcag tatctccaaa
      721 ctaataaggt agttcctacc cttatagtta gcattatata gctaattttc ctaaattatt
      781 tatattcttt attattttat ttaatcctat tgtcttctat ttaacattat acttgtaatt
      841 tatagttatt acaatttact atatactttt tttgatttaa cgttataata taagtgatgg
      901 gtaaatttta aaagttaata ggaggagttt aaaatggcat taattactac aaaagaaatg
      961 tttaaaaaag cttacgaagg tggatttgct ataggtgcat ttaacataag tgacttagaa
     1021 caattacaag gtgttttaaa agctgctaaa gctaaaaatt cttatgttat gatacaagct
     1081 tctatgtcag ctgttaaata tgctggacct catacattag ttgaaatggt taaggctgct
     1141 tctgatgaaa taggtgtaga tgtagctctt cacttagacc atggtccaaa tatggatgct
     1201 attaaaactt gtattgatgc tggtttctct tcagttatga tagatggttc tcattttgac
     1261 tttgaagaaa atgttagaat aactaaagaa gctgttgaat atgcacactc taaaggtgtt
     1321 gttgttgaag cagaacttgg tgttctagct ggaacagaag atgatgttac ttctgatgtt
     1381 cacaagtata ctcaaccagc tgaggcagtt gagttcgttg aaagaacagg tgttgattca
     1441 ttagcaatag ctattggaac ttctcatggt gctttcaaat tcaaaggaga agctaaatta
     1501 agatttgata tactagaaga aatccaaagc aaattaccag gtttcccaat agttcttcat
     1561 ggtgcatctg ctgttgacca aaacgctgtt gctacatgta atgaatttgg tggaaatata
     1621 gctggtgcta aaggtgtacc tgtagatatg ttaagaaaag catcttctat ggctgtttgt
     1681 aaaataaaca tggatactga cttaagacta gctatgactg ctgctatcag aaaattctta
     1741 gctgaaaatc caaaagaatt tgacccaaga aaatacattg gtgctggtag agatgctata
     1801 caagctgtag ttgaaagcaa gatagatgat gttttaggtt ctgctaactc tataaactaa
     1861 tataactttt aaatatttat aaaagaaaag agatgactta aagtaattaa atttattttt
     1921 aagccatctc ttttaagttg tatcaattat gtatcttata atattttaat tatattgtct
     1981 ttttaaactg acagctaaac gtgaatatgt ccactcttta tctttctatg gatggtagaa
     2041 ggatatatac ctataacttc agcagccttt gttattgaac catacttatt tatagcctta
     2101 cttatcatta tttggtccac tattttatat gcatcattaa gattcataat tccatttacc
     2161 actattggtg ataaatcatc tgtttcatta tcaatcatat ctaaatttat cagcatgtta
     2221 aattcatctt ctccaattac atttttagct gataatacta caaatctttc aatgatgttt
     2281 tttaattctc ttatgtttcc tggccaagaa tattcttcta ataactcaat aactttaggt
     2341 gaaacttcta cttctctatt gtacttttca ttgtaaagtt ttaagaaata atttactaat
     2401 ggtactatat cctcttttct ttctctaaga ggaggtattt ttactggaat aacacttaat
     2461 ctatagtaca aatcttgtct aaatttaagg ttatttctaa ggtcttcatg agaaatatta
     2521 gtagcactta tatatctaac atctattttt ataggcttac taccacctat tctagtaata
     2581 gtattttctt gtataaccct aagtaatttt ttttgcatct gcattggtaa ctcacctatc
     2641 tcatccaaga aaatagttcc accattcgcc tcttcaaaaa gtcccttttt accttttttc
     2701 ttagctccag taaaagaacc ttcttcatat ccaaaaaact ctgactctaa taattcattt
     2761 ggtatagctc cacagtttat agctataaaa ggtttgtctc tccttgaact atacttatat
     2821 attgttcttg ctattatttc ttttcctaca cctgattctc catttataaa tatagaagaa
     2881 tctgaggctg cagctctctt aacaactttt tttatattgt ccataatctt actttgactt
     2941 atatactctg tgtcttctga tatatcacta tattttatct gagattcaag cttaataacc
     3001 cttgaaatat ctctccctgt tagtactcct cctgtaagct ttcccttaga atcccatctt
     3061 ggaactgccg tatatgcaat aagcttccca tctgggtaaa cgatatttct ataaatcttt
     3121 ttcttcactt taagcatgtt taatattatt ggttcttcaa ctaatccttg tctcacaagg
     3181 tctgttatat ttctaccaag tacctcagtt cttttaaaag gtaatatctc atcactgaca
     3241 gtgttcatct tttcaattac cccatctttg ttaaacacaa caacaacttc atctatatgc
     3301 tctattatat cttcaattac tttatcagat attagagact tctcaaataa ataaactata
     3361 ttattttcat tattattatc tttcatattg tatatatgtg cagtcatcct taactctctg
     3421 aaagctattt tctttttact atctgtcagt atttctgttt tgtcaaaatt tgaatctaat
     3481 tcatttatat ttctaggctt ttttatatct agaaaactaa aaatactatc tattagatga
     3541 ttacagtata ttatttcgcc tgattcttta cataaaatgg ctggattttc tacatgttct
     3601 ataagcccta ttaaattatt atccattaat attcgcacct ttcattaagt aatttattga
     3661 actacataca aaatttattg tttgcttaca ataaatacca tcagcacata taatcaactc
     3721 cttataatat tcgtcttttc aagtagttta tttcattaaa acgtaactaa gatataattt
     3781 taatgattat agtttgttaa aaatttcatt ttgcttttaa ttgtatttta tcacatattt
     3841 agtaattatt tgtctattta atacaattta attaaattta agatttattt aaaaaaacaa
     3901 agataatcct tacagaatat aactcacaat ttgtaagcaa tatacatata aggattatcc
     3961 agattatctt gtattataaa attttattca aacagacttt ttaataactg acaaatacac
     4021 actagcaata acttatcaaa gggttaataa ttatgttact aactaataag acttattcaa
     4081 tcagtaatat ttatatagtt tgtatctatt aagcttttat agcttctaat aaatcatcaa
     4141 cgctatctaa tttttctata agcattggaa ctatttcatg taagtctcca actactccat
     4201 agtcagcgat ttctaaaatt ggagctgctg ggtttttgtt tatagcaatt atgaaatctg
     4261 atgattgcat acctgctaaa tgttgtattg ctcctgatat accacaagct atatataggt
     4321 ttggtttaac agtagttcct gtttgaccta cttggtgaga atggtctatc catccagcat
     4381 caacagccgc acgagaagaa ccaactactc cacctaattt gtcagctaat ttcttaagca
     4441 tttcaaatcc ttctggtcca cctaatccta aaccacctga tactataaca tttgcatctg
     4501 taagagatac taaatctttc ttagttttaa ctgtttctaa aacagtagtt cttatatcat
     4561 cttgagttat tttgtagtct aatgctataa cttcaccagt tcttgtttcg tctttttcag
     4621 ctttatccat aacaccaggt ctaactgtag acatttgagg tctatggttt ggacaaatga
     4681 ttgtagccat tatgtttcca ccaaatgctg gacgagtttg ttttattttc ttatcttctg
     4741 ggtctatttc taacttagta cagtcagctg ttaatccagt tccaactctt gaagctattc
     4801 taggtgctaa gtctctacct atatgagttg ctccgaaaag cattatttct ggttttattt
     4861 catctattgc atcttttatt acttttgtat atgcatcagt tgtatatttt tctaaaagag
     4921 catcatctgc aacataaact ttgtcagctc caaaagtgat taattcttta gctaatccat
     4981 caacatcttt tcctaataat attgcacaaa gttctgcatc tacttcttta gctaattttc
     5041 ttccttctcc taataattct ataactactg gagctatttt tccttctctt tgttctgcaa
     5101 atatccatac gtttttataa gaacttaaat cttttatatc attcatagtt tgtactcctc
     5161 caacttatat aatgtgcttt tgttttagac cgattattat tttactagct atttcatcag
     5221 ctttaccttc taagatttct ccttttcctc ttggttcagg agtaaatgat ctaaatactt
     5281 tagttggtga tgcttttaaa ccaacttctt ctttatttac atctaaatca tcaattgtcc
     5341 aaacttttac atcatgttta tatgctttta ctattttatc aacagacatg tatctaggtg
     5401 tatttaactc ttttactgct gttaaaagta caggtgtact tacttttatt aattcgtatc
     5461 catcttctaa ttgtctttga actattatat cttttccttc tattttgaaa tcttgtacat
     5521 atgtaacttg aggaatgtct aatttctctg caatttgtgg tccaacttgt gcagtatctc
     5581 catctattgc ttgtcttcct gcaaatatta tgtcatagtc acctacttta gatattcccg
     5641 ctgcaatagt atttgatgta gcccaagtat cagctcctcc aaatgctcta tcacttaata
     5701 atattgcatc atcagcaccc atagctaaac actctcttag cataaagtct gcttgaggag
     5761 gtcccattgt tataactgtt atagttacat catcatgctc atctttaatt tttaaagctt
     5821 cctctaatgc atttgcatca tctgggttta atatacttgg aactccatct cttataagag
     5881 tacctgtttc tttatttatt cttacttcgt tagtatctgg aacttgtttt acgcaaacta
     5941 atattttcaa agtctatacc tccaatttca ttagattatt tattatttta atacgtttga
     6001 tgaaattacc attttttgaa cttctgatgt tccttcgtat attgatacga ttctagcatc
     6061 tctgtacatt ctttcgattt cataatcttt tataaatcca tatccaccgt gaagttgtaa
     6121 tgctttataa gcaacttcat ttgcaatttc tgatgcatag tatttagcca tagcagattc
     6181 ttgagcaact ctcattcctg catctctctt ttgtgctgca tcataaacta atcctctagc
     6241 tgcacaaact tttgtttcca tgtcagctat agtaaattga gtattttgga atttagctat
     6301 aggcttacca aattgaactc tttgttttgt atatttaaca gcttcatcta aagcaccttg
     6361 agctattcct agagctaagg cagcaacacc tattctacca acttctagag tacccattgc
     6421 tatcttaaat ccttgacctt cttttccaag taagttttct tttggaactt taacgttttc
     6481 aaatattaag tcagaagttt cagttcctct tatacccatc ttatcttcat gagctcctgt
     6541 tgagaaacca tcccatttac tttctactat aaacgctgat gttcctctaa gtcctttact
     6601 tctatctgta actgctatta ctatagctac atcacagaat ggtccattag ttatgaaagt
     6661 ttttctacca tttaatatat agtggtctcc aactaattct gctgttgttt gttgtccacc
     6721 tgcatcagaa ccagctcctg gctcagttaa cccaaatgct cctattaatt cacctgttgc
     6781 tatacctttt aagtattttt gtttttgctc ttcagtacca ttgtaaagta ttggtaatga
     6841 agcaagagaa gaaccagctg taacaaaagt agctgttgaa gcacatcttc tagctatttc
     6901 ttccattact atagcgtaag aaacatatcc tgctccccca ccaccatatt cttctgggat
     6961 attactgctt ataaattttg catcagctaa ttttttgatt aattccttag gcattacacc
     7021 tgttttatcc atttctgctg gtaaagtatc taattcttta ctaacaaaat ctcttactgc
     7081 ttttcttaaa agttcttgtt ctttgttata taacatagct tcctccaatt ttcatatttt
     7141 atagtaaatt tattttaatg aacttacgct aaactcatca tctcagcaaa agtttgaata
     7201 cgagttctgg cttgttcatt attctgagtt tgttggtcga tttcaacata taaagtaggt
     7261 attccactat cttctatatc ttttctaact aaaggataat cgtattcttc tgggtcacag
     7321 aatttcatca tacagaagat aacaccatct atatctttct ttttaacttc atctactata
     7381 agtgacccac gttttttctt agggtcataa gctaatgaac atccttctat gtttgaccat
     7441 tgtcttgcta atctctctaa cgcatcatca cctgctggta catctgttct gaattgtctt
     7501 gtttcttgtg ctaagtcgtc agctacaact gatatattgt tgtcttctaa aatgtctaat
     7561 atatctttag aatcagctaa tatacctgtt aataaaactt tttttccaga acagacttct
     7621 tctggcatag catttaattt tgctattaaa tctttcacta gctcagtatg ttcttctttt
     7681 ctcataaaga acccactttt aattactaag cttcttattg atggttttat agtattagaa
     7741 tgtttataag ctacttcaac aaagtctctc atagtttttc tatgttcatt gtaaacttct
     7801 atactttcat gaatttttgc ttcttctatt tcatatccac aaatttcttc aagttctctt
     7861 tttacacctt tgtactcact gattaagtat tttacaccag cttcaagttt tctattttgt
     7921 gggtgtacta atgatatata ttttatatga ggtactgctg atttccagtt ttgtcctaaa
     7981 caaattagtg tatcacacat acctggtata ataactccag ataattcatc ataagcacct
     8041 tttaatccat attctaaaca tgattgcatt attgaacatg caaatgcagg gaaatattgt
     8101 ttagctaaat ctaattctgt ttgtcctccc catataccaa ctggaagcat tccagctgca
     8161 tgtataattt cttctgggca ataaactggg aaacaaccta tagctttttt accagtttca
     8221 tttttatatt tttttacagc cgcatttgga ttttcaacta cttctttcat tttagataaa
     8281 atagcttcca ttatatctca cctctattaa gtttttttct ttcttccatt acttcaacta
     8341 aaccttgaat tcttgtttca aattgagcat tagtgaaagc tctagggtct gcttggtcac
     8401 catcaaatcc agcatatggt aatccagttt cttcagctgc tcttctttgc atttcatatt
     8461 gtattaaact cataagttta cagcttctgt tcatatgata gaatgctcca tcacatttac
     8521 cctctactaa agaatcaact ctatattttg tcatacggtc taggtttaca ttgttaaaca
     8581 tagtactata tgctactgcc attccatcta aatcattaac ttcatattgt aatgcccaag
     8641 catgtgggta aacactacct gtcatgttaa ctccaaattt agctaatgtt ttcatcttgt
     8701 atcctatata tggccaacaa ggtatacctt ccatcattat tctgtatttt tcttcccctc
     8761 tgaaagaaga tttaccagtt ttcatgttgt cttctaattc ttctataagt aacttaaatg
     8821 cttctgtagt ttctttttta cctctagcac aaactattac agccatgtaa gtaaataagt
     8881 caaatccatt cattggagaa ggagaagaat ctgctggtaa actcatagaa tacttccata
     8941 gtcttccgtt ttcagctgat attttcatta cttcttcaaa cttcttaggg tcaaatttct
     9001 ttcctgatat aatttctagt tgttttatag cttcttcaaa ttgagcttta atataatcta
     9061 ttcttgattg agtaacttcg tcttcattat tgaaagttgt atcaatcatt attaaaggta
     9121 tatctaattc tcttgaaata ttttcatacc attttataac ttgattacat atattgttac
     9181 agcaaagtag gaaatctgga gctggcatat ccaaagcttc acatccacca ttttctaaaa
     9241 gaccaaaatt tgttcttgca tatgcacata ggtcaataga atatccttta gattcagcta
     9301 tttcacataa ttctaaagaa ccttttttag ctgctactcc agctgcttgg ttttctggat
     9361 ataatacgtt taagtcaaat acttctgcta actcttgagg aaatactgat gttgaccaac
     9421 ctacaggtct tccttcttct ttagctttaa atgcatttgc atattgttca gctaataaat
     9481 cattaattac tactctagct tctttttttt cagacatttt catccctctt tttatatatt
     9541 tttcacttct ttttgtgatt cttttgcttc atcaaaagca tataacgctg ctcctaaagc
     9601 accagttaat tgaggtatat caggtacaat aatttctgtg ttgatttctc ttgccatagc
     9661 tcttacaata ccactatttc tagcaactcc accaaccata actacatttc tttgtactcc
     9721 tattcttttt actaggctag aaactctctt tgctactgaa gtatgaatac ctgccactat
     9781 atcttcaatt tttgcatttt cagataaatg tgatataacc tcagactctg caaatactgt
     9841 acatgtactg cttattgata cttcattttg agaattcata gatatacttc cgagttcaga
     9901 tacatcaacc tctattattt ttgccattac atctaaaaat cttcctgtac ctgcagcaca
     9961 tttgtcattc ataagaaagt ttaatagtct tccattatta tctaatttta atacctttgc
    10021 atcttgacca cctatgtcaa taatggttct tgtctctgga attatgaaat ttacccctct
    10081 agcatgacag cttaattcac ttatttgctt atcagcatct gaatagttca ttcttccata
    10141 tcctgtaact acaacttttt taatatcttc tcttgcaaga cctgtcttgc catataattt
    10201 ttctaaaact cttgatggtc cagtagtccc agtaccagag gatattgttt cagaagctac
    10261 aatatcttcc ccattcttta agattactcc ctttgatgca gttgaaccta tatctaatcc
    10321 cattgtgtac atctttagca ttctcctttt aaaaaatcta gaattactaa ttaatatctt
    10381 acaactttac tatctttaaa gttatttatt ttctcttcat cgtagcctaa agattttaat
    10441 acttctacag tatgttgacc tacttttggt gctggtgtat attctttaat tccttcattt
    10501 ctaaacataa ctggagtatt aactaagaca cctgtatttc cgctatcgta tgttttctta
    10561 aataagaagt catttgccca agcttgttcg tcatctaata aatcttcaca gctttgaatt
    10621 ttttcaaatg gtaagtctgc ttcttctaat aaagctgacc actcgtctaa tgttttttct
    10681 aacatagctt ctccaactat cttaactaaa tcttcaacat gattaaccat tgaatctatg
    10741 ttattatatc tatcatcttc taatatatat tctctattta taaccttaca gaatttgcct
    10801 aaccacttgt tgtattgtat taaagctaat tgaatccatc ttccatcttt acatttatat
    10861 gtagtcatta atgggctgtt tggattttct cttgataaag gcatttcatt tccgtattgt
    10921 gctgttgtta tcattgttcc cattccatat atagctgtat ggaaaagact tactgttact
    10981 ctctcacctt taccagtttg agctttttta tgtaatgctg ctaaacttcc tgctgctagt
    11041 gctagacctg catagtggtc accaaatcct gctgctgtat ttgctggaga tgttcctttt
    11101 tccataacag attggctaac tcctcctctt gcgaagtatg cagtatagtc aaatcctggt
    11161 ttatctttta aaggtccttt ttcaccatat cctaatattt gagagaatat taatcctgga
    11221 tacttatctt ttatttggtc ataagctata cccatttttt ctaatgcttg aactctaaca
    11281 ttagttacaa atatgtctgc ttctgataat aatttatgta atatttctac tccttctttt
    11341 gattttacat taatacttac acccttttta tttccatttt ctaattcaaa catagggttt
    11401 tcatcatctg atgctggaga tttaaatgtt ccacccataa ctcttattcc atcaccttct
    11461 ataggttcaa tcttaataac ctctgcaccc cagtcaccta acatttttgc acaacatggt
    11521 gctgcgatga aacttgaaag ttctactact ttaactcctt ctaaaagcat aacgttttcc
    11581 tccttaatag tatcaattat aaaatttcga ttaaatcact cataaaatac ttttttctga
    11641 tattaactgt ctgatgtcaa agttcctaat cttatctaaa ataacttaaa aattattaaa
    11701 attatttttt attgttttat tgtcttacac ctatattgtt aagcatttat cgtgccaact
    11761 ttatatttaa tcaaaatatc gcttgatttg aatgtttttt aacaatatgc aattgaataa
    11821 tctttttcat atcgtggcat cttgccacta tccattgcat tttgctattc tttctatgct
    11881 tataaaagtt ttttttatat atttttttat gaaaaagctt gacatttcaa aaaaagtttt
    11941 cattgctaaa ctcaaaaaca catgttatca ttgtaattaa tcagcaagtt agttaactaa
    12001 tttcaaactt agataatttt ttaataatat ttaatctaaa aaataacaat ttctttgata
    12061 aattttagag taattattac attaaataaa aaattactaa atatcaaaat aacacaggga
    12121 aaacaatttc aaaactaaat ttttaacctt aaaaattttg tataaaaaat ataaccttat
    12181 atgctacata ttatttctaa atgaaaaatt accataataa tttaataatt acaggagggt
    12241 attgatatga aaatactagt atttggagca cgcgattatg aagaaccagt aataaaaaaa
    12301 tggtctgaag aacataagga tgttcaagtg gatatttatc ctgaaaacat gactgaagaa
    12361 aatgtagtta aagctaaagg gtatgatggt atatctatac aacaaactaa ctatatagat
    12421 aatccttata tttatgaaac tttaaaagat gctggggtta aagttatagc ttcaagaact
    12481 gcaggggttg acatgataca ttttgattta gttaatgaaa atggacttat cgttacaaac
    12541 gttccttctt attcacctaa tgcaatagct gaattagctg ttactcaagc tatgaacctt
    12601 ttaagaaaga ctcctctagt aaagaaaaaa gtctgtgaag gtgattaccg ttggatagct
    12661 gaacttcttg gaacagaagt tagatctatt acagttggtg ttataggtac aggaaaaata
    12721 ggtgctactt ctgcaaaatt attcaaaggc ctaggagcta atgtaattgc atttgaccaa
    12781 tatccaaata gtgatttaaa tgatatatta acttacaaag attctttaga agaccttcta
    12841 aaagaagctg accttataac attacatact cctttacttg aaggaacaaa acatatgata
    12901 aataaagata ctctagctat aatgaaggat ggagcttaca tagtaaatac tggccgtggt
    12961 ggtttaatta atacagggga tttaatagaa gcactagagt caggaaaaat tagagctgct
    13021 gcccttgata catttgaaac tgaaggattg ttcttaaaca aaaaaatgaa tcctggagaa
    13081 ttaactgacc cagaaataaa taaacttctt tctatggaac aagttatatt cactcatcac
    13141 cttggtttct tcactagtac agcgattgaa aatatagttt attctagttt aagtagtgct
    13201 gtagaagtta taaaaacagg aactgctact aatagagtaa attagtattt tataaatata
    13261 ttttttcatt aattagctat tatctaacaa aatagccttt aatttcccaa atataagaga
    13321 gtcagctcag tatatattga gtagactctc ttatattatt ttttatatta aacttgtact
    13381 cctcttattt ataccaaatt aaatttatat taacttactc taactactcc tactctatac
    13441 attttaccta tttttacttt tctaattata tacatttgaa tgggtacaat ctgtatttta
    13501 caaaaaaaac acctaacaaa ctatgaaaat tgtatgatta atattaatat atttataaga
    13561 aaacataaca attatgttag ctctggattt ttagggaggg agtatattgg aaaaaatgaa
    13621 taaagttact tctaaaaaaa ctacctatat tgttacttca gcattatttg catccataat
    13681 atgcctgaca atagcatata tattacatat tccagttggt ggaaacaatg gatatgttca
    13741 tataggagat gcttttattt atctagctgc aactattttg ccaacaaact atgctatagc
    13801 tgcttctgca attggtgctg gtctagctga tttatctaca ggtgctgcta tctgggtaat
    13861 accaactata atcataaaac caatacttgt attatttttc acttctaaaa gtgacaagat
    13921 aataaataag agaaatatag ttgcttcggt tgtagcaggt atagtaggat tagttttata
    13981 catgtttgct gaaggtataa ttattggaag ttttacaagt gcatttgtaa tgagcttact
    14041 tggcttatta cagccaatag gaagttttat tgtttttata atcttaggta tggcacttga
    14101 taaacttgat tttaaaaaac gttattttaa ttaagaattt aaatttatgg agggaaaatt
    14161 atgaatatct tatatacaat tgaaaatagt atctatgtaa atataactaa tacatgtcca
    14221 tgtagttgtg ttttttgtat aagaaacgaa aaagatgaag ttgcaaatag tggtagttta
    14281 tggttggagc atgagccaag tgtagatgaa gtaaaagaag ccttcaataa atacaattta
    14341 gacgactatg atgaaatcgt attttgtgga tatggcgaac cattaatgag aataaatgaa
    14401 ttgatagagg tagcaaagtt tataaaagaa aaaagtagca taaaaataag aataaataca
    14461 aatggtctaa gtgatttaat acataacaaa aaaacagcta ttctgttaaa agatgttata
    14521 gatacagtat caataagtct taatgcccca aataaagaag cttataatag agttactcaa
    14581 ccaaagtttg gagaaaaatc atttgactat atgttaggtt ttgcaagaga ttgcaaaaaa
    14641 tatataaaag aagtagcttt ttcagtagta gatgaaatat ctccagaaga aatagaagaa
    14701 tctaaacaac tggcaaaaaa acttgatata ccattaagag ttagacataa gaattagcaa
    14761 gtaaaattat aaaacattaa gaatcacctg aatggtcttt caggtgattc ttcctcttgt
    14821 attaacattt tacaaaatag atttcatacc acactaggaa tgtgtaaatc atccaaatct
    14881 ttctccaatt atcagagttt ccactaatgt agtcattaaa aatctcatta atctcatcta
    14941 tgttaaagaa tttatctgct atctcactat tgaatttatc tcggatatcc ttattatatt
    15001 ggtcatcagc catccatata cgaattggca caataaatcc taatttattt ctaaaagcaa
    15061 tctcttctgg taatgttttt gatgcagctg tacgaaaggc aattttattc tcttttcctt
    15121 tcactttata ttctgatggt attctggatg caatatcaaa tattcttgta tctgtaagag
    15181 gcattctgac ttccaatcca actgcagtac tcattttatc tatattaaaa taaatatctc
    15241 cttccaacca tatttgaata tctacatttg acatgttagt aagtgtatct agttcttctg
    15301 tttcttcata aattcctttt accacttcaa taggaagcac atcaggattg tatttcttaa
    15361 gaatttttct tttctcaccc tcattcataa tgtttgtatt tccaatataa aatgtcttca
    15421 aattctctcc ataacactct gccttatggt atatattata tccaccaaaa aactcatctg
    15481 ctccttctcc agagtaacag atatttgtgt tttcagctgc tgccttgcat ccaatagcaa
    15541 aaacgatagc agatgcatct ccagttggtt gctccatatg gtacattaca taaggtactg
    15601 acgcaaaata ctcttcaggt gtaattatat atctcgaatt tttacaccca agaatattag
    15661 ctgtctgttg tgctaatttt gattcatcaa aacgctcttc ctcataacca catgaacctg
    15721 ttttttctat atctgacatt gccaatatat aagatgaatc aacacctcct gataagaata
    15781 actctgcagt ttcattctct gattttacct ctggcataat tttctgaagt gttgtatgaa
    15841 tttcatccgt ccattcctca atagacttgc tgtggtcagg atgaaattca ggtttccaat
    15901 aacgaccaat acatactgtt ttattctgat agacaaggta atgtcctggc ataagcttct
    15961 ttactccacg gaagaatgta ttttcacctg ctacatatgt gaaagacata taaatctgta
    16021 acatgtcttc atttaactct ttcacaaatc ctggttgctc cataatctta cgaatagatg
    16081 aaccatagag aagcttccca ttttcagtct cataataata gaaaggtttt actccaaact
    16141 ggtctcttag gcagaagagt ttcttttcca cagtatccca taatgcaaag gcaaacattc
    16201 catgcatgtg atttgccata tcatatcccc atgtcttata tgcttccact aaaattgctc
    16261 gttcacgctc atctctacat aatgaagaat cgattccaag ttcttcacag agaactttcc
    16321 agtttcgaat atgtccattg tattctagaa tttttatcat atatgtgtct tttcgtgtta
    16381 aatacttttc ttctaaattt atcatatgtg tatcctttcg tattaaaaca tttaacttct
    16441 aaatttatta aatgtttata tatatttatt cacttataat cactgaaaaa tcgacttttt
    16501 tcaaagtaaa ttctttttga attagtacta atctgctctt tattaatggc ataaatatga
    16561 ttatcaagta ttatcagtgt tataacattg aattttttat tcttcataaa tattgccccc
    16621 tttatattac caactaaata taatatatct tatgttactt acatattctt taagaaattc
    16681 tga
//
LOCUS       DAMJWP010000066        16680 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.068, whole genome shotgun sequence.
ACCESSION   DAMJWP010000066 DAMJWP010000000
VERSION     DAMJWP010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16680
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.068"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..1077
                     /locus_tag="PC700_001801"
     CDS             <1..1077
                     /locus_tag="PC700_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDF2581112.1"
                     /translation="SKNIFYNLEKFYLVLNRISDKEIRVICKEIGILLESGCEITKIF
                     EIIESQSSKKVKNLLSIVSNHIQKGNSIAESFQITGIFSKFFISMIKAGETSGNLDII
                     MSDLSNYYDKEYKLKMKIITISIYPIILIILSILSMLFIFVFVIPNFQVVFTNNGIEP
                     PLITRVLMGISTVVTNNLAYIIFSFILFSIGSIYFFITNDNIKKLINSLKFKIPFIKK
                     INQLVATTRFSRALSVLISSGIQIVEAIDMSASVIDNEFIYERLKISNNYIKKGNSIG
                     KSLNLANVFPKLFISMANTGEESGKLDKSLNVINKFYENELNNKIEQIMKFIEPMLIV
                     FMGLIIGTFILAMVVPMFDAITNF"
     gene            1131..1490
                     /locus_tag="PC700_001802"
     CDS             1131..1490
                     /locus_tag="PC700_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HDF2581113.1"
                     /translation="MINLINKKRKGFTLVEMIVVVTILGVISSIALVKYSKVQESAKL
                     NADYTNAANIVTAASMAINDDEKTIDSLSVETLKEKGYLNTVPVPQSTSGKFELVIND
                     SGTDISVNINSKQFYPK"
     gene            1543..2415
                     /locus_tag="PC700_001803"
     CDS             1543..2415
                     /locus_tag="PC700_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HDF2581114.1"
                     /translation="MAIIKKILNLEINDDYIRLVFLRKSHKKVFIDKSIIKEINFDIK
                     NDSNSIEKIKYIEDVVRFVKEEIGKSKILYKNLYFNIQIQDIVIRNVEVLNTKKKRDI
                     LSMIEFEITQYIPININNYSIKYKVINSKNDKLNIQVILFPKSIIQLIKNISENLNMN
                     PKTININFDILQKLINLNLIENFSEEGVFIECKKSQLIINITKDKKIRETYLLSKEGK
                     SYEFIKRLLRDFKYVYYYGIENCFIEEYFKYTDKSIEVNPLRLKDKVKVENNFDADLE
                     DNRINYINSIGMII"
     gene            2416..2859
                     /locus_tag="PC700_001804"
     CDS             2416..2859
                     /locus_tag="PC700_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581115.1"
                     /translation="MKVDKTINFLNGFKKTKPCNKIYKIIIVLILTCILFKSFMYVKS
                     INELEDYINQNKLVDKSKHTLPTTDKTREKFSKIKLNEIKKIYETIGINSITKISIDK
                     NKLEMEGVCSDLDVLNNLRKIKYIKYYYIDSIVRDGEYYRFRIKS"
     gene            2874..3458
                     /locus_tag="PC700_001805"
     CDS             2874..3458
                     /locus_tag="PC700_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581116.1"
                     /translation="MRLNNREKNLLKILLILVIILASDIAVSYLEENLTSLKNEKQNI
                     INKINNQDNYQIKDNKHIDDKYIESVELNKNEELKNNFNNSFLDKKTNKDIVLLVENE
                     LRDIVNVEYINQESSLDNHNKKQVNLEIKVNGELANVLKLEGVMKKLRLNNKLEKIEI
                     NKLKVNNFDVQNIPNEYKQEKDNIVECKMTVRVI"
     gene            3711..4748
                     /locus_tag="PC700_001806"
     CDS             3711..4748
                     /locus_tag="PC700_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HDF2581117.1"
                     /translation="MLGRNELCPCGSGKKYKKCCLNKDIVSERACRKTELSQKQYTDL
                     YSKLYDYSRQDKFKEECEKAKEMFYIMQNDSVNEKFERFFNTYLIQDHIMENKKVMTV
                     GFLEENGANLSQSEVSILKSMFESYVSIYEVKEVSAGKIVLKDCLSGNELCTEDVKLL
                     RSFKIGSCMIARIVDIEGTNILIDITISISNEVKDIILKDIMNLFNQYQDVYKDMKSF
                     LIYHTHILYKYIQQLLDPSIAEYLKRERDKNDTKVNSENELAEEECKVLDTLKENVEK
                     ENIDACIEFWNEYKKNHENIKGAENGWAAAVEYYVKKEAGQPITQVQISKKYDISSST
                     LGKRYKDLKIS"
     gene            4829..5194
                     /locus_tag="PC700_001807"
     CDS             4829..5194
                     /locus_tag="PC700_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581118.1"
                     /translation="MSLSLGNAIAVSTNFIKSNPAVPVYNGEEIYAVIYHIEIGYNNG
                     VSPDTKQYIIIDYKEFDLSMDKKNELINVAKEYCKSKGIVENENLEINLLDNEEAENF
                     LEKVFNNMKVIRGLITKKG"
     gene            5199..5516
                     /locus_tag="PC700_001808"
     CDS             5199..5516
                     /locus_tag="PC700_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581119.1"
                     /translation="MDYIGIENITPYENTYEFSVYEYDDEITLGSEKLYVCELRVVLI
                     KVNSLYVERLHKSVEAMVLVKNLKKDLDKTLVVNKIKNFVLDEIWVENLVKENIEVIF
                     VES"
     gene            5564..7168
                     /locus_tag="PC700_001809"
     CDS             5564..7168
                     /locus_tag="PC700_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581120.1"
                     /translation="MLRVSNVKLGIDKDISLLKDVVLKKLKIKEKNLVKYSIYKESID
                     ARKKGKMEFVYSVDVEVKDENKILNSKIKDVTKIKEIRYINVPVGDKKLKNNPLVIGS
                     GPAGLFAGLLLAQMGYEPIILERGLDVDSRTKDISDFWTTRKLKNNSNVQFGEGGAGT
                     FSDGKLTTRIKDIRCRKVLEELVQFGAPDEILYSHKPHVGTDILKNVVKNIRNEIISL
                     GGQVRFNSKVTDIVVSNDSIQSVIINDTEKIDTDTIILAVGHSARDTYEMLYSRGIKI
                     VQKPFAIGARVEHPQDLINKAQYKDFYNHPRLGAADYRLIEHVSNGRTAYTFCMCPGG
                     SVIASASNDFEIVTNGMSEHARDKINANSAFLVNVTPEDFKSDNPLAGVHFQQKYEKL
                     AFELGGKNYNAPVQLVGDFINNKTSLNLGSVEPSYRPGYTLTNLSECLPSFVTETMRE
                     AFISLDKKLNGFAMYDAVLTGVETRSSSPIRIVRDENTLESVSIKNLYPCGEGAGYAG
                     GIVTAAVDGIKCAEKIIQKYTNNTKI"
     gene            7292..8920
                     /locus_tag="PC700_001810"
     CDS             7292..8920
                     /locus_tag="PC700_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HDF2581121.1"
                     /translation="MNIIISLMGGLGLFLYGMNLMGEGLQKSAGTKLKKIIKLLTSNL
                     FMGVLVGTGVTAVIQSSSATTVMVVGFVNAGIMTLKQAIGVIMGANIGTTVTAQLVSF
                     DLTGMAPVALGVGIILYLFGNKPRIKNIAEILIGFGILFTGMDFMKMAVEPLRDYQGF
                     TDLLVTFGRYPLLGLLLGFGITAIIQSSSASMGMLVALAAEGLVPLSAALPILYGQNI
                     GTCVTSLLSSIGANKNARRAAMMHLIFNVLGTVIFLIFLNKPVVSMVTSWDPSNVARQ
                     IANTHTLFNIISVLILLPFTNLIIKLAIKLVPDRAGDIDEDETKTIKYIDDRMIETPS
                     IALANTIKEALRMGEKAKESLNASMEALVEHSTEKIAKTYRRERLINDLQKAILNYLL
                     KLSKAPLDDDSREVVDTLFNTVNDIERIGDHAKNIAELSQVAIDSNISFSEEGQSELD
                     VMYNRVVSAYTYALESMRTDNVDLACKVIKIEEQVDIMEKSCRANHMYRLNNNLCSIE
                     NGVIYLDVISNLERISDHAVNIAQQVIAKRLGND"
     gene            9185..10534
                     /locus_tag="PC700_001811"
     CDS             9185..10534
                     /locus_tag="PC700_001811"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDF2581122.1"
                     /translation="MGKINFDYSKATEFFCQNEIDVMQPYVDVAHDMLHNKTGLGNTF
                     LGWIDLPKNYDKEEFDRIKKSAEKIKSDSDVLLVIGIGGSYLGSRAAIDMVSHSFRNG
                     LKKEQRKAPEVYFVGHNISSTYIMDLLDIIEGKDISVNVISKSGTTTEPALAFRIFKD
                     YLEKKYGKEEARKRIYATTDASKGALRQLATEEGYETFVIPDDVGGRFSVLTAVGLLP
                     IAAAGLDIDAMMKGANDAREAFQNPDLKSNDSYRYAVARTILHRKGKDVELLVNYEPQ
                     LHYVSEWWKQLYGESEGKENKGLFPASVDFSTDLHSMGQYIQDGKRLLFETVLNVENC
                     KRNITISSEEVDLDGLNYLAGKTVDFVNHKAFEGTLLAHTDGKVPNLVINIPQLDEYN
                     FGYLVYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLGKPGYEKEKEELEKRLK"
     gene            10690..11769
                     /locus_tag="PC700_001812"
     CDS             10690..11769
                     /locus_tag="PC700_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDF2581123.1"
                     /translation="MSRRKKGISLVILVAIISSILSSFLTIILVKDNLVSKSTGSSTP
                     IVVNDDGKSQNIYQAVAEKATPSVVGITTTSVDTSNMFAIPTETQGVGTGIIVDSNGY
                     ILTNSHVISDGQATSVNVLFNDGSTTSGKVVWFDQQLDLAIVKVDKTGLTPAEFADSD
                     KVKVGDISIAIGNPLGLDFQKTVTQGIISGLDRTIQTEKTNMTGLLQTDASINAGNSG
                     GPLLNQKGQVIGINTAKASQAEGLGFAIPINTAKSIVEEVIKNGKYEKVTLGIKGTDV
                     SNYEAATGTKLSTDKGVYVAEVISGSSAEKAGVKVGDIITKVGDTDITGMNDLNKKLY
                     TFSKGASTKITVNRGGKAVTINVNF"
     gene            12114..13022
                     /locus_tag="PC700_001813"
     CDS             12114..13022
                     /locus_tag="PC700_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HDF2581124.1"
                     /translation="MNPLVINLQKCSIHDGPGIRSTVFFKGCPLECVWCHNPESQTYT
                     KQVLYNEERCSKCEACINICPHKAIYKGETKICLDQDKCEFCETCLDYCVNNAREIVG
                     QEYSVRDLVKEIEKDRIFYEESGGGVTLSGGEVMAQDMDFICGVINMCKSKGIHVAID
                     TCGYAKSENYERVAKCADLFLYDIKLIDEDKHIKFTGKSNDLILKNVKILSELGVNIN
                     IRIPLIVGVNVDDENLEVKKMIEFLKPLNIQAVSLLPYHNIGKHKYDKIYKKYEGEEL
                     QRPSEEKLEEIKRLFEASNFNTKIGG"
     gene            13027..15396
                     /locus_tag="PC700_001814"
     CDS             13027..15396
                     /locus_tag="PC700_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HDF2581125.1"
                     /translation="MARGTFERTKKLREESINAEPHISIERAVLMTEAYKKYEGSVEI
                     PVLRALSFKHYIENRTLSINDGELIVGEKGDSPNGAPTYPEICCHTMEDLEVMHNRDI
                     INFSVSEEARKIHKEEIIPFWKKRQTRDKIINAMTPEWLAAYEAGMFTEFMEQRAPGH
                     TVCGDTIYKKGFLDLKKDIEARLKELDFLNDLDAYNKKADLEAMAIACDAMVILGKRY
                     AEKARQMAEEETDEAKKKDLLLIAETCDVVPAHKPETYHQAIQMYWFVHIGVTTELNI
                     WDAFTPGRLDQHLNPFYERDVENGILDRDRAQELLECLWVKFNNQPAPPKVGITLKES
                     STYTDFANINTGGINPDGQDGVNEVSYIILDVMDEMKLIQPSSNVQISKKTPQKFLKR
                     ACEISRKGWGQPAFYNTEAIVQELMEAGKTIEDARLGGTSGCVETGCFGKEAYVLTGY
                     MNIPKILELTLNNGYDPISKKQIGIETGDPRNFQSYEELFEAFKKQLHYMIDIKIEGN
                     AVIENICAKHMPCPLMSTIVDDCIEKGKDYQRGGARYNTRYIQGVGIGTITDSLTAIK
                     YNVFDKKKFDMDTLLKALDANFEGYEAILNLVSNKTPKYGNDDDYADEIMQEIFNAYY
                     NEVTGRLTVCGGEYRVDMLPTTCHIYFGEIMGASPNGRLCAKPVSEGISPEKGGDTNG
                     PTAVIKSCAKMDHIKTGGTLLNQRFAPSVVQGEKGLDNMANLVRAYFNMDGHHIQFNV
                     FDKNVLLEAQKNPKDYKDLIVRVAGYSDHFNNLSRTLQDEIIGRTEQTF"
     gene            15721..16524
                     /gene="proC"
                     /locus_tag="PC700_001815"
     CDS             15721..16524
                     /gene="proC"
                     /locus_tag="PC700_001815"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDF2581126.1"
                     /translation="MKTLGFIGSGNMGSAMIGGIVQSGLVKAEDITVSDLSQVALDKV
                     KEEFGINTTTDSKVAVTNSDVVIVALKPNICEKALTPLKELIDANKIIVSIAAGKTIE
                     SLESFIGSDKKIVRVMPNTPALVGEGMSALCKNSNVTDEELNMIKALFESFGEAEIVS
                     EYLMDTVTGISGSSPAYVFMFIEAMADAAVLAGMPRQQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPAGTTIEAVKVLEEEGFRAAVIKSIVACIDKSKDMSK"
BASE COUNT         6901 a         1683 c         2972 g         5124 t
ORIGIN      
        1 tctaaaaata tattttataa tttagaaaag ttttatttag tattaaatag aatcagtgat
       61 aaagaaatta gagtaatttg caaagagata ggcatattgc tggaatctgg atgtgaaatt
      121 acaaaaatat ttgaaatcat tgagagtcag tcaagtaaaa aagtaaagaa tttgcttagt
      181 atagtatcaa atcatattca aaaaggtaac tcaatagcag aatcatttca aataacaggt
      241 atattttcta aattctttat aagtatgata aaagcaggag aaactagtgg aaacttagat
      301 ataataatga gtgatttatc taattattat gataaagaat acaaattaaa aatgaaaata
      361 ataactattt caatatatcc aataatactt ataatattat ctatcttatc aatgttgttt
      421 atattcgtat ttgtaatacc taattttcag gtggttttta ccaacaatgg aattgaacca
      481 cctttaataa ctagagtttt aatgggaata tctacagtgg ttacaaataa cttagcttat
      541 ataatattta gttttatatt gttttcaata ggaagcattt atttctttat aacaaatgat
      601 aatataaaaa aattgattaa ttcgttaaaa ttcaaaatac cttttataaa aaaaattaat
      661 cagttagtag ctacaacaag attttcaagg gcattgtccg ttttaattag cagtggaata
      721 caaattgtgg aagctataga catgtcagct agtgttatag acaacgaatt tatttatgaa
      781 aggttaaaga tttctaataa ttatataaaa aaaggaaata gcataggtaa atctttgaat
      841 ttagcaaatg tttttccaaa attatttatt tcaatggcaa atacaggaga agaaagtggg
      901 aaactagata aaagtctaaa tgtaataaat aaattttatg aaaatgaatt aaataacaaa
      961 atagagcaaa taatgaaatt tatagaacct atgctaattg tatttatggg attaattatt
     1021 ggcactttta ttttagcaat ggttgttcct atgtttgatg caattactaa tttttagaga
     1081 gaaaaataaa ttttattaga tgaaactttt gaaaaggaga gaatatttaa ttgataaatt
     1141 tgataaataa aaaacgaaaa ggatttacac ttgttgaaat gattgtagta gtaactattt
     1201 taggcgttat atctagtata gcattagtta agtatagtaa ggttcaagaa agtgccaaat
     1261 taaatgcaga ctatacgaat gctgctaata tagtaactgc agctagcatg gcaattaatg
     1321 atgatgaaaa gacaatagac tctctaagtg tagaaacatt gaaggaaaag ggatacctaa
     1381 atactgttcc agttcctcag agtacatcag gtaaattcga acttgtcata aatgatagcg
     1441 gaacagatat aagcgtaaat ataaattcta aacaatttta tccaaaataa tattttatat
     1501 tgattgctgt aagttttaaa attattttta tttaagaagg agatggctat aattaaaaaa
     1561 attctaaact tagaaataaa tgatgattat ataaggcttg tatttttaag aaaaagtcat
     1621 aaaaaagttt ttatagataa atcaattata aaagagatta actttgatat aaaaaatgat
     1681 agtaattcga ttgagaagat taaatatata gaagatgtag ttagatttgt aaaagaggag
     1741 attggtaaat caaaaatatt atataaaaat ttatacttta acattcaaat tcaagatata
     1801 gtcattagaa atgttgaagt tctaaatact aagaaaaaaa gagacatatt aagcatgatt
     1861 gaatttgaaa taacacaata cattccaata aacataaata actattcaat aaaatataaa
     1921 gttataaatt ctaaaaatga taaattgaat atacaagtaa ttcttttccc aaagagtatt
     1981 attcaattaa ttaagaacat ttcagaaaat ctaaatatga atccaaagac tataaatata
     2041 aattttgata tattacaaaa gcttataaac ttaaatttaa tagagaattt ttcagaagaa
     2101 ggggtattta tagaatgtaa aaaaagtcag ttaattataa atataacaaa agataaaaaa
     2161 ataagagaaa catacttgct atcaaaagag ggaaaatcat atgagttcat aaaaagatta
     2221 ttaagggatt ttaagtatgt ttattactat ggtatagaaa attgttttat tgaagaatac
     2281 tttaaatata cagataaatc tattgaagta aatccattaa gattaaaaga taaagtaaaa
     2341 gtagaaaata attttgatgc agatttagaa gacaatagga taaattatat aaatagtata
     2401 ggaatgatta tttaagtgaa agtggataaa acaataaatt ttcttaatgg atttaaaaaa
     2461 actaaaccat gtaataaaat ctataaaata ataatagtct taattttaac gtgtatttta
     2521 tttaaatcat ttatgtatgt gaaatctata aatgaacttg aagattatat aaatcaaaat
     2581 aagctagtgg ataaatcaaa gcacacttta ccaacaacag ataaaactag agagaaattt
     2641 tctaaaataa aacttaatga aataaaaaaa atatatgaaa caattggaat aaatagtatt
     2701 actaaaatat cgatagataa aaataagcta gagatggaag gtgtgtgtag tgatttagat
     2761 gttttaaaca acctaaggaa aataaagtac ataaaatatt attacataga cagtattgta
     2821 agagatggag aatattatag atttagaata aaaagttaga taggtgatta tttttgagat
     2881 taaataatag agaaaaaaat ctacttaaaa tattgttaat attagttatt atattggcaa
     2941 gtgatatagc agtaagttat ttggaagaaa acctaactag tttgaagaat gaaaagcaaa
     3001 atataataaa taaaataaat aatcaagata attatcaaat aaaggataat aagcatatag
     3061 atgataaata tatagaaagt gttgaattaa ataaaaatga agaattgaaa aataatttta
     3121 ataactcatt tttagataaa aaaacaaata aagatatagt gttattagta gaaaatgaac
     3181 tgagagatat tgtgaatgta gaatatatta atcaagaaag tagtttagat aatcacaata
     3241 aaaaacaagt taatttagaa ataaaagtta atggagaact tgcaaatgtg ttgaaacttg
     3301 aaggtgtaat gaagaagtta agactaaata ataaacttga aaagatagaa attaacaaat
     3361 taaaagtcaa taattttgat gttcaaaata ttccaaatga atataaacaa gaaaaagaca
     3421 atattgttga atgtaaaatg acagttagag tcatataaca aaaaagaata gataaagtaa
     3481 aaaactatta tcaattaata aaattttata gaggaattag ttttacgata attcatttct
     3541 acaagaggga ttgttgatga ttttatcgaa atcctaatta aagttaatat attgtaccaa
     3601 aatataagtg aatttaatag aatatttact tatactatag attgattaat aaaaaaatgg
     3661 tataataaca aattgtactt taatttaata ataagggagt ggaagtcata ttgttaggga
     3721 gaaatgagct atgcccttgt ggaagtggca aaaaatacaa gaaatgttgt ttaaataaag
     3781 atattgtttc agaaagagca tgcagaaaaa ctgaactttc acaaaaacaa tatacagatt
     3841 tatattcaaa gttatatgat tattcaagac aggataagtt taaagaagaa tgtgaaaaag
     3901 ctaaagaaat gttttatata atgcaaaatg atagtgtaaa tgaaaaattt gaaagatttt
     3961 ttaatactta tttaatacaa gaccacataa tggaaaataa aaaggttatg acagttggtt
     4021 ttcttgaaga aaatggtgca aacctaagtc aatcagaagt atcaatatta aaaagtatgt
     4081 ttgaatcata cgtaagtatt tatgaggtaa aagaagtttc tgctggaaaa atagtattaa
     4141 aggactgttt aagtggaaat gaactttgta cagaagatgt aaaacttctg agaagtttta
     4201 aaatcggaag ttgtatgata gctagaatag ttgatattga aggaactaac atattgatag
     4261 atataactat aagtatatca aatgaggtta aagatataat tttaaaggat attatgaatc
     4321 tattcaatca atatcaagat gtatacaaag atatgaagtc gttcttaatt tatcatacac
     4381 atatactata caagtatata caacagcttt tagacccaag tatagctgaa tatttaaaga
     4441 gagaaagaga taaaaacgat acaaaagtta attcagaaaa tgaattagct gaagaagaat
     4501 gtaaagtttt agatactctt aaagaaaatg tagaaaaaga aaatatagat gcatgtatag
     4561 agttttggaa tgaatataag aaaaatcatg aaaatataaa aggagctgaa aatggttggg
     4621 cagctgctgt tgaatactat gttaaaaaag aagcaggaca acctataact caagttcaaa
     4681 tatcaaagaa atacgatata agctctagta cactaggaaa gagatacaaa gatttaaaaa
     4741 tatcttaatt aattataaaa agctttaagt catagataaa gaatctatag tataatatat
     4801 gtataatgtt tagaaagagg tattaataat gagtcttagt ttaggaaatg caattgctgt
     4861 atcaacgaac tttataaaat ctaacccagc tgtacctgtg tacaacggag aagaaatata
     4921 tgcagttatt tatcatatag aaataggata taataatggt gtatcacctg ataccaaaca
     4981 atatataata atagactata aagagtttga tttatctatg gataaaaaaa atgaactaat
     5041 aaatgttgct aaagagtact gtaaatctaa aggtattgta gaaaacgaaa accttgaaat
     5101 aaacctttta gacaatgaag aagcagagaa cttcttagaa aaggtgttta ataacatgaa
     5161 ggttataaga ggtcttataa ctaaaaaagg ataaataaat ggactatata ggaatagaaa
     5221 atataacacc ttatgaaaat acatatgaat ttagtgtata tgaatatgat gatgaaatca
     5281 ccttaggtag tgaaaagtta tatgtgtgtg aattaagggt tgtattgatt aaagttaatt
     5341 ctctgtatgt tgaaagattg cataaatcag ttgaagcaat ggtcttagta aaaaacttga
     5401 aaaaggattt agataaaaca cttgttgtaa acaaaataaa gaattttgtg ctagatgaga
     5461 tttgggtaga aaatctagta aaagagaata tagaagttat atttgtagaa agctaggaat
     5521 aaccctagct tttttatatt acaatctaag agaggaaaac aatatgttaa gagtatctaa
     5581 tgtaaaactt ggaattgata aagatatatc tttattaaaa gatgttgtac ttaagaagct
     5641 aaaaataaaa gaaaaaaact tagtcaaata tagtatatat aaagaatcta tagatgctag
     5701 aaaaaaagga aaaatggaat ttgtttattc tgtagatgta gaagtcaaag atgaaaataa
     5761 aatacttaac tcaaaaataa aagatgtaac aaaaataaag gaaatcagat acataaatgt
     5821 acctgtgggt gataaaaaat taaagaataa tcctttggta attggtagtg gaccagctgg
     5881 tttatttgca ggtctgcttt tagctcaaat gggttatgaa ccaatcatac ttgaaagagg
     5941 tcttgatgta gacagtagaa ccaaagatat aagtgatttt tggacaacta gaaaattaaa
     6001 aaataattca aacgttcaat ttggagaagg tggagcagga actttttcgg atggtaaact
     6061 tacaactaga ataaaagata tacgatgtag aaaagtatta gaagagttag ttcagtttgg
     6121 agcaccagat gaaatattat actcacataa gccacatgtt ggaacagata tattaaaaaa
     6181 tgtagttaaa aatataagaa atgaaataat aagtttaggt ggtcaagtta gatttaattc
     6241 aaaagtaaca gatatagttg tatcaaatga ttctatacaa tctgtaataa taaatgatac
     6301 tgaaaaaata gatacagata caattatact agctgttgga catagtgcaa gagatactta
     6361 tgaaatgttg tattcaagag gtataaaaat agttcaaaaa ccttttgcaa taggagctag
     6421 agttgaacat ccacaagatt taataaataa agcacagtac aaggattttt ataatcatcc
     6481 taggttagga gctgcagatt atagacttat agagcatgtt tcaaatggaa gaacagcata
     6541 tactttttgt atgtgtccag gaggtagtgt catagcatct gcatcaaatg attttgaaat
     6601 agttacaaat ggtatgagtg aacatgcaag agataaaata aatgcaaata gtgcattttt
     6661 agtaaatgta acgccagaag attttaagag tgataatcca ctagcaggtg ttcatttcca
     6721 gcagaagtat gaaaaattag cttttgaatt aggtggaaaa aattacaatg caccagttca
     6781 gcttgttgga gattttataa acaataagac ttctttgaat ttaggaagtg ttgagccatc
     6841 atatagacca ggatatacac ttacaaattt aagtgaatgt ttgcctagct tcgtaacaga
     6901 gactatgaga gaagcgttta tatctcttga taaaaaattg aatgggtttg caatgtatga
     6961 tgctgttttg acgggagtag aaactaggtc atcatcacca ataagaatag taagagatga
     7021 aaatacttta gaatcagtga gtattaagaa tctatatcca tgtggtgaag gcgctggata
     7081 tgcaggtggt atagttacag cagcagttga tggtataaaa tgtgcagaaa aaataataca
     7141 aaaatacacc aataatacaa aaatttaaca taaacttaat gattttttaa tatttaacat
     7201 gaatttaaca ttatcactcc tataatttga taaaatacaa taggttataa aatttttaga
     7261 tacagtaatc tctttacaag gaggaaataa tttgaacatt ataataagtc taatgggtgg
     7321 attaggtctg ttcttatatg gtatgaacct tatgggagaa ggtcttcaaa aatctgctgg
     7381 gacaaagcta aaaaagataa taaaactact tactagtaac ctatttatgg gagttttggt
     7441 aggtacagga gttacagcag taatacaaag ttcaagtgct actactgtaa tggttgttgg
     7501 tttcgtaaat gcaggaataa tgactttaaa acaggcaata ggagttataa tgggtgctaa
     7561 tatagggact actgttacag cccaattggt atctttcgac ttaactggta tggccccagt
     7621 agcattaggt gtaggtatta tactttattt atttggaaat aagcctagaa taaaaaatat
     7681 agccgaaata cttataggtt ttggtatatt atttacaggt atggatttta tgaagatggc
     7741 agtggagcca ctaagagact atcaaggatt tactgaccta cttgttacat ttggtagata
     7801 tccactactt ggattattat tgggatttgg gattacagca ataatacaaa gttcaagtgc
     7861 atctatgggt atgttggttg cactggcagc agaaggtcta gtgccattat cagcagcact
     7921 gccaattctt tatggccaaa atattggtac atgtgtaact tcactgttat caagtattgg
     7981 tgctaacaaa aatgcacgaa gagcagctat gatgcatcta atcttcaatg ttttaggaac
     8041 ggtgatattc ttaatattct tgaataaacc agtagtaagc atggttacaa gttgggatcc
     8101 aagcaatgtt gcaagacaaa tagccaatac acatacattg tttaatataa tatctgtatt
     8161 gatactttta ccatttacaa atcttattat aaaactagct ataaagcttg ttcctgatag
     8221 agcaggtgat atagatgaag atgaaactaa aactattaaa tatatagatg atagaatgat
     8281 agaaactcct tctatagctc ttgcaaatac gataaaagaa gcacttcgta tgggtgagaa
     8341 ggcgaaggaa agcctaaatg catctatgga agccttagta gaacattcaa ctgaaaaaat
     8401 agctaagaca tatagacgag agagattaat caatgattta cagaaagcta ttttaaatta
     8461 cttacttaaa ctttcaaaag cacctttaga tgatgattca agagaagttg tggatacttt
     8521 gtttaataca gtcaatgata tagaaagaat tggtgaccac gctaaaaata tagctgaatt
     8581 atcgcaagtt gcaatagatt caaatatttc tttctcagaa gaaggacaat ctgaacttga
     8641 tgttatgtat aatagagttg tttcagcata tacttatgca ttagaatcaa tgagaactga
     8701 taatgttgat ttagcttgta aggttataaa aattgaagaa caagttgata taatggagaa
     8761 atcttgtaga gctaatcaca tgtatagatt gaacaataat ctatgtagta tagaaaatgg
     8821 agttatttat ttagacgtaa taagtaattt agagagaata tctgaccatg ctgtcaatat
     8881 agcacaacaa gttatagcga agagattagg aaatgattaa aaataattaa tttaaaataa
     8941 tataaaattt aaaatagagg ataaataaaa gagaaacttt aaatgatacg tttagagttt
     9001 ctttttttgt aaatttattt gactaaaatt tttaaattta tacatagtta tagtatttag
     9061 aagaatcgat ttcctgataa taaatgatga gatatgtatg attttgtggc aaacttttaa
     9121 aaaaaacacc tatattttgt gttataatct ctttaaaacg aatatgctga aaagagggat
     9181 ttaaatggga aaaataaatt ttgattatag taaagctaca gagttttttt gtcaaaacga
     9241 aatagatgtc atgcaaccat atgtagatgt tgcgcacgac atgttacata ataaaactgg
     9301 tttaggaaat acttttttag gatggataga ccttcctaaa aattatgata aagaagagtt
     9361 tgacagaata aaaaaatctg ctgagaagat aaaatcagac tcagatgttc ttctggtaat
     9421 aggtatagga ggctcttatt taggttcgag agctgctata gatatggtaa gtcattcttt
     9481 taggaatgga cttaaaaaag agcaaagaaa ggctccagaa gtatattttg tagggcataa
     9541 tataagctca acatatatta tggatttatt agatataatt gaaggaaagg atatttctgt
     9601 aaatgtaata tctaaatcag gaactactac agaaccagct ctggctttta gaatatttaa
     9661 agattactta gagaaaaagt atggtaagga agaagcaaga aagagaattt atgcaacaac
     9721 agatgcaagt aaaggtgcac ttagacagtt agctacagaa gaaggatatg agacttttgt
     9781 aataccagat gatgtaggtg gtcgtttttc tgtattgaca gcagttgggt tacttcctat
     9841 tgctgctgct ggacttgata ttgatgctat gatgaaaggc gcaaatgatg ctagagaagc
     9901 atttcaaaat cctgacttaa aaagtaatga tagttataga tatgctgttg ctagaacaat
     9961 tttacataga aaaggaaaag atgtagagtt attagtgaac tatgaacctc aactacatta
    10021 tgtaagtgag tggtggaaac agctttatgg agaaagtgaa ggaaaagaaa ataaaggttt
    10081 attccctgct tctgtagatt tctcaacaga tttacattct atgggtcaat acatacaaga
    10141 tggaaaaagg cttttatttg agacagtact taatgttgaa aactgtaaga gaaatataac
    10201 tataagttca gaggaagtag acttagatgg tttaaattat ttagcaggta agactgtaga
    10261 ttttgtaaat cataaagctt ttgaaggaac tctattggct catacagatg gaaaagtacc
    10321 taatttagta ataaatatac ctcaactaga tgagtataat tttggatatt tagtttattt
    10381 ctttgagaag gcttgtggaa taagtggata tttattggga gttaatccat ttgaccaacc
    10441 aggagtagaa gcttacaaga aaaatatgtt tgctttacta ggcaaaccag gttatgaaaa
    10501 agaaaaggaa gagctagaaa aaagacttaa ataattttaa atagttaagg tttaattaag
    10561 attaaaataa tataatgaaa tcatcgaaaa ggagtttact caaaatagat gatttcattt
    10621 tttttatgaa aaattgggaa aaatatattt tgataattta tattatatta agatagggag
    10681 tggtatgaga tgtcaagaag aaagaaaggg ataagtttag taatattagt tgctataata
    10741 agttcaatac ttagtagttt tctaacaata attttagtta aggacaattt agtaagcaaa
    10801 tctacaggaa gttctacgcc gatagttgta aatgatgatg gtaagagtca aaatatttat
    10861 caagcagttg cagaaaaagc tacgccatct gttgtaggta ttacaacaac tagtgttgat
    10921 actagtaata tgtttgcaat accaactgaa acacaaggtg taggaactgg aatcatagtt
    10981 gattcaaatg gatatatact tactaattct catgttatat cagatggtca agctacaagt
    11041 gtaaatgtat tattcaatga tggttcaaca acttcaggta aagtagtatg gtttgaccaa
    11101 caacttgatt tagctatagt taaggttgat aaaactggac taacaccagc agaatttgct
    11161 gatagtgaca aagtaaaagt tggagatata tcaatagcaa taggtaatcc tcttggatta
    11221 gattttcaaa aaacagttac tcaagggata ataagtggat tagatagaac tatacaaaca
    11281 gaaaaaacta atatgactgg tttattacaa actgatgcaa gtataaatgc aggaaatagt
    11341 ggaggaccat tgttaaatca aaaaggtcaa gtaattggta taaacacagc aaaagcttct
    11401 caagcagaag gattaggatt tgcaatacca atcaatacag ctaagagtat agttgaagaa
    11461 gtaatcaaaa atggaaaata tgaaaaagta actttaggca taaaaggtac agatgtatca
    11521 aattatgaag cagcaacagg tacaaaatta agtactgata aaggtgttta tgttgcagaa
    11581 gtaatatctg gttcatcagc agaaaaagct ggagtaaagg taggggatat tataactaaa
    11641 gtaggagata cagatataac aggtatgaat gatttaaata aaaagttgta tactttctca
    11701 aaaggtgcta gtacaaaaat aacagtaaat agaggcggaa aagcagtaac aataaatgtg
    11761 aatttctaaa tatgaccaga aagaaggaga ctacatggta aagtgtagtc tctattttta
    11821 tttaattttt atagtatata catctaaatt aattaaaatg atgtattgtt tatagtttat
    11881 tgctatattt tcttataaaa ataaatttaa taaatattta aatttaattt acaaaaacaa
    11941 tataaatatc ataataaaat aaaatttttt aatattacta aaaatatata gacaaaataa
    12001 taacaaatat agtattatgt aattgtagaa atgcttttca taggtatatt ctatgaagat
    12061 gaattgcata aaaatgaaaa aatggaatgg atattattta ggggtgaaaa atcatgaatc
    12121 cattagttat aaacttacaa aaatgtagca tacatgatgg accagggata agaagtacag
    12181 tattttttaa agggtgtcca cttgaatgtg tttggtgtca taatccagag agtcaaacat
    12241 atacaaaaca agttttgtat aatgaagaaa gatgttcaaa atgtgaagca tgtataaata
    12301 tatgccctca taaggcaatt tacaaaggtg aaacaaaaat atgcttagac caagataagt
    12361 gtgagttttg tgaaacttgc ttagactatt gtgtaaacaa tgcaagagag attgttggtc
    12421 aagagtattc tgtaagagac ttggttaaag agatagagaa ggatagaatt ttctatgaag
    12481 aatcgggtgg tggagtaaca ctatcaggtg gagaagtaat ggctcaagat atggacttca
    12541 tttgtggagt aataaacatg tgcaaatcaa agggaatcca tgtagcaatt gatacatgtg
    12601 gctatgcaaa gtcagaaaat tatgaaagag ttgctaaatg tgcagatttg tttttatatg
    12661 atattaagct tattgatgag gataagcaca taaaatttac aggaaaatca aatgacttaa
    12721 tactgaaaaa tgttaagatt ttaagtgaat taggggttaa cataaatata agaataccat
    12781 taattgtagg agtaaatgta gatgatgaaa atttagaggt taaaaaaatg atagagtttt
    12841 taaagccttt gaatatacaa gcagtaagtc ttcttccata tcataatatt ggtaaacaca
    12901 agtatgataa aatttataaa aagtatgaag gagaagaact acaaagacca tcagaagaaa
    12961 aattagagga aataaaaaga ctgtttgaag caagtaattt caatactaag attggagggt
    13021 aataaaatgg caagaggaac ttttgagaga actaaaaaat taagagaaga aagtattaat
    13081 gcagaaccac acatatctat tgaaagagca gtacttatga cagaagctta taaaaaatat
    13141 gagggaagtg tagagattcc tgtacttcgt gctttatcat ttaagcatta tattgagaat
    13201 agaactctta gcataaatga tggagaatta atagtaggtg aaaaaggtga ttctcctaat
    13261 ggagcaccaa catatccaga aatttgttgt cacacaatgg aagatttaga agtaatgcac
    13321 aatagagata taataaattt tagcgtatca gaagaagcta gaaaaataca taaagaagaa
    13381 ataattcctt tttggaaaaa aagacaaaca agagataaga taataaatgc aatgacacct
    13441 gagtggttag cagcatatga agcaggtatg tttacagagt ttatggaaca aagagcccca
    13501 ggacatacag tttgtggaga tacaatatat aaaaaaggat ttttagattt aaagaaagac
    13561 atagaagcaa gattaaaaga gctagatttt cttaatgatt tagatgcata taataaaaaa
    13621 gcagatttag aagctatggc aatagcttgt gatgcaatgg ttatattagg taaaagatat
    13681 gctgagaaag caagacaaat ggcagaagaa gaaacagatg aagctaagaa aaaagattta
    13741 ttactaattg ctgaaacttg tgatgttgta ccagctcaca aaccagaaac ttatcatcaa
    13801 gctatacaaa tgtattggtt tgttcatata ggggttacta ctgaacttaa tatatgggat
    13861 gcttttactc caggaagact tgaccaacat ctaaatcctt tctatgaaag agatgtagaa
    13921 aatggaatac tagatagaga tagagcacaa gaattacttg aatgtttatg ggttaaattt
    13981 aacaatcagc cagcaccacc aaaagttggt ataacattaa aagaaagtag cacatataca
    14041 gattttgcaa atataaacac tggtggaata aatccagatg gtcaagatgg tgttaatgaa
    14101 gttagttata taatacttga tgttatggat gaaatgaaat tgatacaacc tagttcaaat
    14161 gttcaaataa gtaaaaagac tcctcaaaaa ttcttaaaga gagcttgtga aatatcaaga
    14221 aaaggatggg gtcaaccagc attttataat actgaagcaa tagttcaaga gcttatggaa
    14281 gctggaaaaa ctatagaaga tgcaagactt ggtggaacca gtggttgtgt tgaaactgga
    14341 tgttttggta aagaagcata tgttctaact ggatatatga acattccaaa aatacttgaa
    14401 ttaacattaa ataatggata tgacccaatt agtaaaaaac aaataggtat tgaaactgga
    14461 gaccctagaa atttccaatc ttatgaggaa ctatttgaag catttaaaaa gcaattacat
    14521 tatatgattg acataaaaat agaaggaaat gctgtgatag aaaatatatg tgcaaaacac
    14581 atgccttgtc cacttatgtc tacaatagtt gatgattgta tagaaaaagg caaggattac
    14641 caaagaggtg gagcaagata caacactaga tatatacaag gtgttggtat aggaactata
    14701 acagatagtt taacagcaat aaaatataat gtatttgata agaaaaaatt cgatatggat
    14761 acattgctta aagcacttga tgctaatttt gaaggatatg aagctatact taatttagtt
    14821 tcaaataaga ctcctaaata tggaaatgat gatgattatg ctgatgaaat aatgcaagag
    14881 atattcaatg catattacaa tgaggtaact ggtagactaa ctgtttgtgg tggagaatac
    14941 agagtagata tgttaccaac aacttgtcat atatactttg gagaaattat gggagcaagt
    15001 ccaaatggta gactttgtgc aaaaccagtt tcagagggaa tttcacctga aaaaggtgga
    15061 gatactaatg gacctacagc agttataaaa tcttgtgcta agatggacca cataaaaact
    15121 ggtggaacat tattaaatca aagatttgca ccatcagtag ttcaaggaga aaaagggtta
    15181 gataatatgg ctaatctagt aagagcatac tttaatatgg atggtcatca tatacaattt
    15241 aatgtatttg ataaaaatgt actgttagag gctcaaaaga atcctaagga ttataaagac
    15301 ttaatagtta gagttgcagg atatagtgac catttcaata atttaagtag aacactacaa
    15361 gatgagataa taggaagaac tgagcaaaca ttctaggttt gcttgtaaat aaaacttctt
    15421 actatataaa atcgtaactg atataataaa gtataaatgg tttgattcca tttttaaaat
    15481 tttagattaa ttatgagcta actttctaaa taggaagtta gctcgttaag tttaatgaaa
    15541 aaatcgttaa atttatattt agtaaatcat acacatatta gtaagtaata catacaaaag
    15601 aataatttat tatagttata ataaggttat attttataaa aataagataa ttttaagcag
    15661 ttaaaataaa gtcaatttag ctactttaaa ataaaactaa tagaaaaaag gagatttgat
    15721 atgaaaactt taggatttat tggttcaggg aatatgggaa gtgcaatgat aggtggcata
    15781 gtacagtcag gattagtaaa ggcagaagat ataactgtat cagatttaag ccaagttgcc
    15841 ttagataaag taaaagaaga atttggaata aatactacaa ctgattcaaa ggtagctgtt
    15901 acaaattctg atgtggtaat agtagcactt aaacctaata tatgcgaaaa ggctttaact
    15961 ccacttaaag aattaataga tgcaaataaa ataatagtat ctatagcagc aggtaaaact
    16021 atagaaagtc tggaaagttt tataggttca gataaaaaaa tagtaagagt aatgcctaat
    16081 actccagccc ttgttggcga gggaatgtca gctctttgta aaaatagtaa tgtaacagat
    16141 gaagagttaa atatgattaa ggctctgttt gaatcttttg gtgaggcaga aatagtatca
    16201 gaatatttaa tggatacagt tacaggtatt agtggttctt ctcctgcata tgtatttatg
    16261 tttatagaag ctatggcaga tgcagcagta ttagctggaa tgccaagaca acaagcttat
    16321 aaatttgcat ctcaagcagt aatggggtca gctaagatgg ttcttgaaac tggaaaacat
    16381 ccaggtgaat taaaagatat ggtttgttct ccagcaggaa ctactataga agctgttaag
    16441 gtattagaag aagaaggctt tagagcagca gttataaaat ctattgttgc ttgtatagat
    16501 aagtctaaag atatgagcaa ataacagatg taattaagtt tactatatgg tttcttgaat
    16561 tgttttttta gtacttattt atatctatac ataaatgtat ttggtataag taaaataaga
    16621 gtaatttact ataaaaagga atttaatgat tgagtgagaa tttattactt aaatattaat
//
LOCUS       DAMJWP010000067        16639 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.069, whole genome shotgun sequence.
ACCESSION   DAMJWP010000067 DAMJWP010000000
VERSION     DAMJWP010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16639
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.069"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            185..1264
                     /locus_tag="PC700_001816"
     CDS             185..1264
                     /locus_tag="PC700_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2581127.1"
                     /translation="MSRVKNVVGLLGTKGVDALYLTKKTNVNYISGFPDEEAYAVICK
                     DGNFLVTDSRYMELAEKVCKDFEIINWHNFDRSVAKAVKSVCDKVGIKKLGFERTNIV
                     FDKYEELKNLIEKDNGELIPTENIVETLRYVKDKDEIKNTRKACEIADKALEELIPHI
                     KAGVSEIELATKLEYFMKMNGAQNIGFETILISGAKTSLLHGKPSDKIIEKGDFVLID
                     YGAMYNGYISDTTRTFIVGGASEKQLEIYNLVKEAQNVGVENMKAGVHATIPDAEIRK
                     VVKKYEDYYYQGIGHGVGRDVHEEPFIGNYGDKIIEEGCIITMEPGIYFPGWGGVRIE
                     DTVLITKNGPERLTKFPKDLMILDK"
     gene            1377..1976
                     /locus_tag="PC700_001817"
     CDS             1377..1976
                     /locus_tag="PC700_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581128.1"
                     /translation="MYKKHKFIKNIKKAWYKIKLIDRCLIIVMAILMFQSIYNLFVNE
                     VNSQDTTTIDVVVRTTSAAIFGYFLSANFIKRPLRKYTNTDVSSNLFIDNNLPPKGQE
                     SSQNNIMNVKNTIGFTSEADNYEKKIPINNNEGFEEGETSELQIIIATVICIAALTVL
                     FVVRNFTTTTSASLGTISQMRDFVSGCVGFLLGCPSKPD"
     gene            complement(2163..3035)
                     /locus_tag="PC700_001818"
     CDS             complement(2163..3035)
                     /locus_tag="PC700_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2581129.1"
                     /translation="MNINYLYYFQTVCKYKNMTKAAESIHISQPSITLAIKELEKELG
                     FELFYRIGNKIELTPEGKIFLDKSKHFIKQFEDFQCDALDLGKKRKASLKIGIPTVLG
                     TFLLSKILPRFNVIYPDIELKIFEVPTFVGAKMIEESTLDFCIGIIDSDIYDDIDSKT
                     IYKTELYLVTNPKNELAKHPIISNYMLKNVPFVILSEGSYHYKIITKRLEKAKPNIIL
                     HSNQLSTIRYLLENDLASTILYKEIFQNTENLCSIPLERAITANIGVLWRRNQYISHS
                     MKLFIEYMASIHIN"
     gene            3452..4855
                     /locus_tag="PC700_001819"
     CDS             3452..4855
                     /locus_tag="PC700_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581130.1"
                     /translation="MKGSDLVKFELMDFLMNPFVLMFAAVITGILFGKIKFGKFNFGV
                     SGALFTGLFIGWLAYSLGNLIIAKGETAAGYKAATVMMGNGIISSDFFDFFLIIFVAA
                     VGLLAAKDMKAVLKKYGARFVILGVLITFIGGSMTYAMTLLSSDKGSSAYEVSGVYTG
                     ALTSSPGLAAALETAGKHAEDVSKEFEKASIKDKKEILKVVDPEGKLDVNTTTSLTQE
                     QIDKYIAYAEAGVGIGHAVAYPFGVLIVILGVNFLPKLFRMDLKEERRKYEKEMKEAR
                     DSVSGKTIPEVPFNIITFFLTCLAGYLVGGIHVFMGPLGYFTLGATGGSLIVSLVLGY
                     IGKIGVVNFRMEEKVLNILKQIGLVFFLAIVGLRYGGKVVDSIMTSGMHLALVAIAVG
                     VTAMMIGFLVGKYVFKLNWILLSGAVCGGMTSTPGLGAAVDALDSDDPAAGYGATYPF
                     ALLTKVILVIVLHKLPM"
     gene            4882..6426
                     /locus_tag="PC700_001820"
     CDS             4882..6426
                     /locus_tag="PC700_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HDF2581131.1"
                     /translation="MNNEGAFEELSGRLRNKELCDLIMNADEAAKIIEDGMVIGVSGF
                     TPSGYPKAVPLAVSERAKSGEKIKLTVYSGASLGPEVDGAWSEAGIIERRLPYQTNSI
                     LRNNINKGVVDYIDMHLSHSTQFLNYGTIPKVDVAIVEALAITEEGNIIPTSGIGNSP
                     SFIKSADKVIVEINLAKPMEMEGMADIYITENPPNRKPIEINHPKDRIGTTYIPCGLD
                     KIAGVVITNMQDKTRPLGVVDEASKKISNNIIAFLREEVKAGRLSKNLLPLQSGVGSV
                     ANAVLYGLCESEFENLTCYTEVVQDSMLDLIRMGKVTMASTTSVSPSPEGLIKFEKDI
                     DFFKDKIILRPQEISNNPEIARRIGVIAMNTAIEVDIYGNVNSTHIMGSKMMNGIGGS
                     GDFARNGAITIFSTESIAKNGDISSIVPMVSHVDHTEHDVMVIVTEQGYADLRGLAPR
                     ERAIKIIENCAHPDYKEQLRDYLNRACKSGAKQTPHILDEALSWHSKFMSTGTMKKAE
                     TFKSAL"
     gene            6457..7848
                     /locus_tag="PC700_001821"
     CDS             6457..7848
                     /locus_tag="PC700_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HDF2581132.1"
                     /translation="MEKAVENFEDLSKEYINGYIERARKAQREFECYTQEQVDKIVKI
                     VGKVVYYNAEYLAKLAVEETGMGVYEDKVAKNKSKAKVIYNNLKDKKSVGIIDIDRET
                     GITKVAKPVGVVAAITPCTNPIVTPMSNAMFALKGRNAIIITPHHKAIGCSTKTVEMI
                     NEELEKIGAPENLIQILDQQSRENTRNLISSADVVIATGGMGMVKAAYSSGKPALGVG
                     AGNVQCIIDRDVDIKEAVPKIIAGRIFDNGIICSGEQSVIVAEEMFDKIMDEFKNNKG
                     FIVRDKVQKEAFRNAMFVNKSMNKDAVGQSVHTIAKIAGVEIPEDTKIIVIEADGPGE
                     EDIIAKEKMCPVISAYKYKSFEEGVAIAKANLNVEGKGHSVSIHSNTVKNIEYAGENI
                     EVSRFVINQCCATSAGGSFFNGLAPTNTLGCGSWGNNSISENLDYKHLINISRIAYYM
                     PENEVPTDEELWG"
     gene            8041..9537
                     /gene="abfD"
                     /locus_tag="PC700_001822"
     CDS             8041..9537
                     /gene="abfD"
                     /locus_tag="PC700_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyryl-CoA
                     dehydratase/vinylacetyl-CoA-Delta-isomerase"
                     /protein_id="HDF2581133.1"
                     /translation="MALMTGAQYIESLRKLNTKVYMFGEEVKNWVDHPMIRPSINCVA
                     ATYDLAHDPEYADLMTVTSNITGEKINRFGHLHQSVDDLIKKVKMQRLCGQKTASCFQ
                     RCVGMDAFNAVYSTTFECDKAHGTNYHDNFVKYLTYIQENDLVVDGAMTDPKGDRSLS
                     PSAQPDPDMFLHIVERREDGIIVRGAKAHQTGSINSHEHLIMPTISMTEADKDYAVSF
                     AVPSDAEGVFMIYGRQSCDTRKLEEGADVDLGNKEFGGQEALVVFDNVFVPNDRIFLC
                     GEWDFSGMLVERFAGYHRQSYGGCKVGVGDVIIGAAALAADYNGANKASHIKDKLIEM
                     THLNESLYCCGIACSSEGHKTEAGNYQIDLLLANVCKQNVTRFPYEIVRLAEDIAGGL
                     MVTMPSEKDFKSDLKVGTSGMTIGEVCNKYFKASSVASTEERMRILRFLENICLGSSA
                     VGYRTESMHGAGSPQAQRIMISRQGNINQKKELAKKIAGIKKEEALNL"
     gene            9672..9947
                     /locus_tag="PC700_001823"
     CDS             9672..9947
                     /locus_tag="PC700_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581134.1"
                     /translation="MTCGVRFCGGCNPRYERGKALTIIKEHFNGRVDFSIAQENVEYD
                     LLLIIGGCATLCASYQQYKVKNECVKMWDFSHIERVINVIESAICET"
     gene            9971..11278
                     /locus_tag="PC700_001824"
     CDS             9971..11278
                     /locus_tag="PC700_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HDF2581135.1"
                     /translation="MSWQELYQSKLCSATEAVKQIKNGDTVVFAHCVGEPPALVEAMI
                     ENAEQYKDVEIKHMVSLGSGGYTAKGMEAHFRVNPMFVSGNVRKAIENGDGDFTPAFF
                     HEVPKLLREKRLKCDVVLAQVTPPDEHGYCSLGTSVDYTYEAIKTARTVIVQVNDQFP
                     RTYGEVVHVSEFDYIVEKSQPLFELQPAKIGEVEEAIGKNCASLIEDGSTLQLGIGGI
                     PDAVMLFLTDKKDLGIHSEMISDGTLALYEKGVINGKYKNFDKEKMTVTFLMGTKKLY
                     DFANNNPAVEVKPVDYVNHPAIIMKQHKMVSINSAIQVDLMGQVVAEAMGLRQFSGVG
                     GQVDFIRGVSMGEDGKAIIAMPSITTKKDGTVISKIVSIVDEGAPITTSRNDVDYIVT
                     EYGIAELKGKSLRERARNLINIAHPSVRESLAVEFEKRFKEKY"
     gene            11298..12416
                     /locus_tag="PC700_001825"
     CDS             11298..12416
                     /locus_tag="PC700_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate dehydrogenase"
                     /protein_id="HDF2581136.1"
                     /translation="MEALRVVPKIFYFDTFKEFNEEFKIGKNDLVITNEFIYEPYMKP
                     LGIDTNLIFQEKFGTGEPSDEMIDSMTKEMKKYNFDRIIAFGGGTIVDICKILALDVP
                     EKSIDLFEGEVSPKKVKELVVVPTTCGTGSEVTNVAIAELKSKHTKKGLAVEETYADY
                     AVLIPETVKGLPYKFFVTSSVDALIHAIESYLSPKASPFTEMYSLQAIKMIMDGYKKI
                     VDKGEEERFNHLRDFVLASNYAGIAFGNAGCAAVHALSYSIGGAFHVAHGEANYQFFT
                     EVFKMYSRKKPNGKIIKCTKILADALECDPNCDVYGELEKFLNKLIAKKALREYGMVE
                     SQIDEFTDSTIANQQRLLANNYVELSREEIREIFANLY"
     gene            12703..13623
                     /locus_tag="PC700_001826"
     CDS             12703..13623
                     /locus_tag="PC700_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HDF2581137.1"
                     /translation="MYKSENFSKKSVLINSFLSKFLKIIGWGLVSLFTLSALVLSTIS
                     IIIGWAVILPIQVCAVFLLIGGVCIFKGKRLGDQISRYKKYCTIINNRNIIPVELIAE
                     NTSKSLNFIIKDVQKMIDKNYFINTYIDKRNNQIVLTNEDFIPPRYEDVAYEVKEEEK
                     TDESNEVDTIINKGMNYLTQIKEANKNIKSESMGDKIVQVEDVTSKIFDVVKHDPSKL
                     TQIQKFMDYYLPTTLKLLNSYHTLEEQGIDRENITTTMESIENTMNTIVVAFENQLDY
                     LFEDEAIDISTDITVLENMLVQEGLTSGKF"
     gene            13738..14895
                     /locus_tag="PC700_001827"
     CDS             13738..14895
                     /locus_tag="PC700_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDF2581138.1"
                     /translation="MNNLDDIPVMPTLTLDPFGEESNTNDIDNSDLLMKKDEKDPEEE
                     KLSESERKMVKEFAEKIDITNTNMILQYGAGAQKKVASFSETALKSVKTRDLGEVGDM
                     LTNLVTDLKAFSADEKEQSGFLGIFKKANNKISNLKTKYDSAEVNVDKVSKELQKHQV
                     KILKDIAMLDKMYELNLAYSKELTMYIIAGKQKLKDMKEYEMPKLREKARLSGSTEDA
                     QSVNDMVSLCDRFEKKIHDLELTRMVSLQMAPQIRLVQNSNNLMAEKIQSTIVNTIPL
                     WKNQIVLALGISHLNQAMKAQREVSNMTNELLLKNAETLKMGTIETAKESERGIVDID
                     TIKKTNQSLISTIDEVVKIQHDGRIKRQEAEVELSKIENELKSKLLEFTVK"
     gene            complement(14980..16479)
                     /locus_tag="PC700_001828"
     CDS             complement(14980..16479)
                     /locus_tag="PC700_001828"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDF2581139.1"
                     /translation="MAFYFDTPSHTFSEYLLVPGFSSTDCIPANVSLKTPVTKFKKGE
                     EADIYMNIPLTSAIMQSVSDDKMAIALAKEGGISFIYGSQTIENEAAMVARVKSHKAG
                     FVVSDSNIKPDNTLKDILDLKEKTGHSTVAVTEDGTSTGKLLGIVASRDYRISRMDLD
                     TKVSEFMTPMSSIVYANKDVTLKEANNIIWDHKLNSLPVLDDNGNLMYMVFRKDYSSH
                     KENPLELLDSSKRYVVGAGINTRDFAERVPALVEAGADVLCIDSSEGFSEWQKITLDF
                     IREKYGDTVKVGAGNVVDREGFLYLAEAGADFVKVGIGGGSICITREQKGIGRGQATS
                     IIEVAQARDEYFEKTGIYIPICSDGGIVYDHHITLALAMGADFIMLGRYFSRFDESPT
                     NKVNINGSYMKEYWGEGSARARNWQRYDLGGDKKLSFEEGVDSYVPYAGSLKDNVTLS
                     LSKVRSTMCNCGALSIPELQKNAKLTLVSSTSIVEGGAHDVMLKDNRNL"
BASE COUNT         6422 a         1952 c         3037 g         5228 t
ORIGIN      
        1 gctaatttat tatatttatt caattaaaat taagctttct aactaaataa aaactaatga
       61 agtattaaaa tgaaattaat aaaatatgaa aaaattttag gaataaagta tctgttagat
      121 aaaaataaga ttaattagtg atataatata attaagataa aatttattat ttggaggtaa
      181 aatcatgagc agagtaaaaa atgtagtagg attgttggga actaaaggtg ttgatgcact
      241 atatctaaca aagaaaacta atgtaaacta tataagtggt tttcctgatg aagaagcata
      301 tgcagtgatt tgcaaagatg gaaacttttt agttactgat agtagatata tggagcttgc
      361 agaaaaggtt tgcaaagatt ttgaaattat aaattggcat aattttgata gaagtgtggc
      421 aaaggcagtt aaaagtgtat gtgataaagt gggtataaag aaacttgggt ttgaaagaac
      481 taatatagtg tttgataaat atgaagaact taaaaatctg attgagaagg ataatggaga
      541 attaatacct acagaaaata ttgtagagac tttgagatat gtaaaagaca aagatgaaat
      601 caaaaataca agaaaagctt gtgaaatagc ggataaggca ttagaagaat tgattccaca
      661 tataaaagct ggagttagcg aaattgaact ggctacaaag ttagagtact ttatgaaaat
      721 gaacggagca caaaatatag ggtttgaaac tatactgata tcaggagcaa agacttcatt
      781 gcttcatggt aagcctagtg ataagattat agaaaaaggt gattttgtac ttatagacta
      841 tggtgctatg tataatggct atatatctga tacaactaga acttttatag ttggaggagc
      901 ttctgaaaag caattagaaa tatataattt agttaaggaa gctcaaaatg taggggttga
      961 gaacatgaaa gctggtgtac atgcgacaat tccagatgct gagataagaa aagttgtcaa
     1021 aaaatatgaa gactattact atcaaggtat aggacatgga gtcggtaggg atgttcatga
     1081 agaacctttc attggtaatt atggtgataa aatcatagaa gagggatgta taataacaat
     1141 ggaaccaggt atttatttcc ctggatgggg tggagttaga atagaagata ctgttttgat
     1201 tactaaaaat ggtccagaaa gacttactaa attccctaaa gatttgatga ttttagacaa
     1261 ataatttaat ttatatatat ttttttacat aaaatataac ataatctcac tattaagctc
     1321 tatttacata ttatagtaaa gagggcttaa ttttattgta agaggtgata gtatatatgt
     1381 ataaaaaaca taaatttata aaaaatataa aaaaagcctg gtataaaatc aaattaattg
     1441 atagatgcct tataattgta atggcaatat taatgtttca atctatatat aatttatttg
     1501 ttaatgaagt aaattcacaa gatactacaa ctattgatgt agtagtaaga accacttcag
     1561 cagctatatt tgggtatttt ttaagtgcta attttataaa gagaccttta aggaagtata
     1621 caaatactga tgtatcttca aatcttttta tagataataa tttaccccca aaaggtcaag
     1681 agtctagtca aaataatata atgaatgtca aaaataccat tggatttact tcagaagctg
     1741 ataattatga aaagaaaata cctattaata ataatgaagg ttttgaagaa ggagaaacta
     1801 gtgaattgca aattattata gctactgtaa tttgtatagc agcattaaca gtactttttg
     1861 ttgtaagaaa ttttacaact acaacatcag cttctttagg tactatttca cagatgagag
     1921 attttgtctc aggatgcgtg ggttttttgc taggttgtcc aagtaaacct gattaaaaaa
     1981 gtattatcaa taaaaggtat tttaaaaagt atcagtataa taatatttag atttaaatgt
     2041 atacaattat aattctataa cattctatat tgtattttta tactgatact ctaaactata
     2101 ctatttatta aaatatttta acattgtatt taaacaaaat attatatctg tataatataa
     2161 atttagttta tatgaataga tgccatatac tcaataaaaa gtttcatact atgagatatg
     2221 tattgatttc ttctccaaag aactccaata tttgcagtaa ttgcacgttc taatggtatt
     2281 gaacagagat tttctgtatt ttgaaatatt tctttataca atattgttga agccaagtca
     2341 ttttctagta aataacgtat ggtagaaagt tgatttgagt gtaaaattat atttggctta
     2401 gctttttcaa gtctttttgt aataatctta tagtgataag aaccttcaga taagattaca
     2461 aaaggtacat tttttaacat ataatttgat ataatgggat gttttgccaa ttcatttttg
     2521 ggatttgtaa ctaagtacaa ttctgtttta tagattgtct ttgaatctat atcatcataa
     2581 atatcactgt caattatacc aatacaaaaa tcaagtgtag attcttcaat cattttagct
     2641 ccaacaaatg ttggaacttc aaaaatttta agctctatat ctggataaat tacattaaat
     2701 ctaggaagta tttttgaaag caaaaacgta cctaaaactg taggaatacc tattttgaga
     2761 gaagcttttc tcttttttcc taaatcaagt gcgtcacatt gaaaatcctc aaattgtttt
     2821 ataaaatgtt ttgacttgtc taaaaaaatt tttccttcag gcgttaattc tattttatta
     2881 cctattctgt aaaaaagttc aaagccaagt tctttttcta attctttaat tgctaatgtt
     2941 atggatggtt gagaaatatg aatactttcc gcagccttag tcatgttttt atatttacag
     3001 acagtttgaa aatagtataa atagtttata ttcatttggt attagttcct ttcatttaca
     3061 tattagtgac aagtatataa ttaacaatag cataagtttt gtatattgtt gtaaaaaaat
     3121 agaatagttt taaactatca tagcataagt ttttgatatt ttcaatctat taagaatagt
     3181 gctattatta aattaacaaa agagaatagc tggtatggtt tagagtaaat caatctatat
     3241 tacagttata tcaacgaata tcaaaaaatt atctattaga aaataaaaat tatttttaca
     3301 attaaattgt taattaaatc ataaagaaaa aagtgatatt aacaagataa aattttttta
     3361 atcagaaatg ttaatttaat aacaaaatag agtattgggg agaaaggaga aatatatttt
     3421 ttaataatat ttgttaattt aataacgaaa aatgaaaggg agtgatttag tgaagtttga
     3481 attgatggat tttttaatga atccttttgt ccttatgttt gctgcagtaa ttacaggaat
     3541 tttatttgga aaaattaaat ttgggaagtt taattttggg gtatcaggag cattatttac
     3601 aggattattt attggatggc ttgcatatag cttggggaat ctaataatag caaaaggaga
     3661 aacagcagca ggatataagg cagcaactgt aatgatgggg aatggaatta tttcttctga
     3721 cttctttgat ttctttttaa taatatttgt tgcagcagtt ggtctattgg cagcaaaaga
     3781 tatgaaagct gtattaaaga agtatggagc aagatttgta attttaggag ttcttataac
     3841 ttttataggt ggctctatga cttatgcaat gactttacta tcaagtgata aaggaagtag
     3901 tgcttatgaa gtttctggcg tttacacagg agcattaaca agttcgcctg gattagctgc
     3961 tgctttagaa acagctggta aacatgcaga agatgtatca aaagaatttg aaaaagcttc
     4021 aataaaagat aaaaaagaaa ttttaaaagt agtcgaccca gaaggcaaat tggatgtgaa
     4081 tacaactaca tcactgactc aagagcaaat agataagtat attgcatatg ctgaagctgg
     4141 tgttggtata ggtcatgcag tcgcatatcc ttttggagtt ttaatagtaa tcttaggtgt
     4201 aaatttcctt ccaaaattat ttaggatgga cctaaaagaa gaaagaagaa aatatgagaa
     4261 ggaaatgaag gaagcaagag atagtgtttc tggtaaaacg ataccagaag ttccatttaa
     4321 tataataaca tttttcttga catgtttagc tggatactta gttggaggaa tacatgtatt
     4381 tatgggacca ctagggtact tcacactagg cgcaacagga ggttcactaa ttgtatctct
     4441 agttcttgga tatataggta agataggggt agtaaatttc agaatggaag aaaaggtttt
     4501 aaatatttta aaacaaatag ggctagtatt tttcttagca atagttggat taagatatgg
     4561 tggaaaagta gttgattcaa ttatgacatc tggtatgcac ctagccttag tagcaatagc
     4621 agtaggtgtt acagctatga tgataggatt cttagttggc aaatacgtat ttaaattaaa
     4681 ttggatttta ttatctggag ctgtctgtgg tggtatgaca tcaacaccag gtcttggagc
     4741 tgctgttgat gctcttgata gtgatgaccc agcagcaggt tatggtgcta cgtatccatt
     4801 tgcattatta actaaagtta tattggttat agttttacac aaacttccaa tgtaaataag
     4861 attttatatg gggggatatt tatgaataat gaaggagcat ttgaagaatt aagtggaaga
     4921 ttaagaaata aagaattgtg tgatttaata atgaatgcag atgaagctgc taaaattata
     4981 gaggatggca tggttattgg agttagtgga tttacacctt caggctatcc aaaagcagta
     5041 ccattagcag tttcagaaag agctaaaagt ggagaaaaga taaaattaac cgtatattca
     5101 ggagcatctt taggcccaga agttgatgga gcatggtcag aagcaggtat aattgaaaga
     5161 agacttcctt atcaaactaa ttcaatactt agaaataata ttaataaagg tgttgttgat
     5221 tatatagata tgcacctaag tcattctact caatttttaa attatggaac tatacctaaa
     5281 gtagatgtag ctatagtaga agcacttgca ataactgaag aagggaatat tataccaact
     5341 agtgggatag gtaattctcc atcttttata aaaagtgcag ataaagtaat agtagagata
     5401 aatcttgcaa aaccaatgga aatggaaggt atggcagata tatatattac ggaaaatcca
     5461 ccaaatagaa aaccaataga aataaatcat cctaaggata gaattggaac aacatatata
     5521 ccttgtggtt tggataaaat tgcaggagta gtaattacca atatgcaaga taagacccgt
     5581 ccattggggg ttgtagatga agcttcgaaa aaaatatcaa ataatattat tgctttttta
     5641 agagaagaag taaaggcagg cagattatca aagaatttac ttcctcttca atctggtgta
     5701 ggaagtgtag caaatgcagt attatatgga ttatgtgaat ctgaatttga aaatttaact
     5761 tgttatacag aggttgtaca ggactctatg ttggatttaa taagaatggg taaagttaca
     5821 atggcatcta ctacatctgt aagtccatct ccagaaggat taataaaatt tgaaaaagat
     5881 attgattttt ttaaggataa gattatatta agacctcaag aaattagtaa caatccagaa
     5941 atagccagaa gaattggtgt aattgcaatg aacactgcaa ttgaagttga tatttatgga
     6001 aatgtaaatt ctactcacat aatggggtca aagatgatga atggaattgg aggttctggt
     6061 gattttgcta gaaatggagc aataactata tttagtactg agtcgattgc aaaaaatggt
     6121 gatatatcat caatagtacc tatggtatct cacgtagacc acactgaaca tgatgtaatg
     6181 gtaattgtaa cagaacaagg atatgctgat ttaagaggtc ttgcacctag agaaagagct
     6241 ataaaaataa tagaaaattg tgcccatcca gattataaag aacaacttag ggattattta
     6301 aatagagctt gtaaaagtgg agcaaaacaa acacctcaca tattggacga agctttatca
     6361 tggcatagta aatttatgag tacaggtaca atgaaaaagg ctgaaacttt taaaagtgca
     6421 ttataaaata tttaataaat tggaggtaga ttttaaatgg aaaaagcagt ggaaaacttt
     6481 gaagatttaa gtaaggaata cattaatggt tatattgaga gagcaagaaa agctcaaaga
     6541 gagtttgaat gttatactca agaacaagta gataaaatag taaaaatagt tggaaaagta
     6601 gtgtattata atgctgaata tttagctaaa cttgctgtag aagagactgg aatgggagta
     6661 tatgaagata aggtagctaa aaataaaagc aaagcaaaag ttatatataa caatttaaaa
     6721 gataaaaaat cagttggtat aattgatata gatagagaaa ctggaataac aaaggtagct
     6781 aaaccagttg gagtagtagc ggcaataact ccttgtacaa atccaatcgt tacacctatg
     6841 agcaatgcaa tgtttgcact taagggaaga aatgcaatta ttatcacacc tcatcataaa
     6901 gcaattggat gtagtacaaa aactgtagag atgattaatg aagaattaga aaaaatagga
     6961 gctccagaaa atttaataca aatacttgac caacaatcaa gagaaaatac aagaaattta
     7021 atttcatcag cagatgtagt aattgccact ggaggaatgg gaatggtaaa agctgcgtac
     7081 tcaagtggaa aaccagcact tggtgtaggg gcaggaaatg ttcaatgtat catagataga
     7141 gatgtagaca ttaaagaagc agtacctaaa atcatagcag gaagaatttt tgataatggt
     7201 atcatttgtt caggtgaaca gtcagtaata gtagcagaag aaatgtttga taaaataatg
     7261 gatgaattta aaaataacaa aggttttata gttagagata aagtacaaaa agaagctttc
     7321 agaaatgcta tgtttgtaaa taaatctatg aataaagatg cagtaggtca atcagttcat
     7381 acaattgcaa aaattgcagg tgttgagata ccagaagata caaaaataat agtaatagaa
     7441 gctgatggac caggagaaga ggatattatt gcaaaagaaa aaatgtgtcc agttatatct
     7501 gcatacaagt ataaaagttt tgaagaaggt gtagctattg caaaagcaaa tttaaatgta
     7561 gaaggaaaag gacatagtgt atctatacat tcaaatacag ttaaaaatat agaatatgca
     7621 ggagaaaata tagaagtatc aagatttgtt attaatcaat gttgtgctac tagtgctgga
     7681 ggaagtttct ttaatggtct tgcacctact aatactctag gttgtggttc ttggggaaat
     7741 aacagcatct ctgaaaatct tgattataag catctaatta atatttctag aattgcttat
     7801 tatatgccag aaaatgaagt ccctacagat gaagaacttt ggggataaaa cttagacaaa
     7861 aaatattcat tctaaattaa agtaggaata tattttaatt tagaatgaat ttaagacata
     7921 aattaaattt aaattagttt attggactaa atttaaaata ttaagagaca tatagaagaa
     7981 ggaagtatta taatttaaaa taaataaaaa aatctataaa agagaaggag tggttttatt
     8041 atggcattaa tgacaggagc tcaatatatt gaaagtttga gaaaattaaa tactaaggtt
     8101 tatatgtttg gtgaagaggt taagaattgg gttgaccatc ctatgattag accatctata
     8161 aattgtgtag cagcaactta tgacttagct catgacccag aatatgcaga tttaatgaca
     8221 gttacttcaa atatcacagg tgaaaaaata aatagatttg gtcatcttca tcaaagtgtt
     8281 gatgatttga taaaaaaagt aaaaatgcaa agactatgtg gtcaaaaaac agcttcttgt
     8341 ttccaaagat gtgttggtat ggatgcgttt aatgcagtgt attcaacaac atttgagtgt
     8401 gataaagccc atggaactaa ttatcatgat aattttgtaa aatacttaac ttatatacaa
     8461 gaaaacgatt tagtagttga tggagctatg actgacccta agggagatag aagtctatca
     8521 cctagtgctc aacctgaccc agatatgttc cttcatatag tagaaagaag agaagatggg
     8581 ataattgtaa gaggagcaaa ggcacatcaa actggttcaa taaattcaca tgagcacctt
     8641 ataatgccta ctataagtat gactgaagca gataaagact acgctgtatc atttgcagtt
     8701 ccttcagatg ctgaaggtgt gtttatgatt tatggaagac aatcttgtga caccagaaaa
     8761 ttagaagaag gtgcagatgt tgatttagga aataaggagt ttggaggtca agaagcttta
     8821 gtagtatttg ataatgtatt tgttccaaat gatagaattt tcttatgtgg agaatgggat
     8881 ttctctggta tgttagtaga gagatttgca ggataccata gacaaagtta tggtggatgt
     8941 aaagtaggag taggtgatgt aataatagga gcagcagctt tagcagctga ttataatgga
     9001 gcaaacaaag cttcacatat aaaagataaa ctaatagaaa tgactcacct taatgaaagc
     9061 ttatattgct gtggtatagc atgttcatca gaaggtcaca aaacagaagc tggaaactat
     9121 caaatagatt tacttcttgc aaatgtgtgt aaacaaaatg taactagatt tccttatgaa
     9181 atagtaagac ttgcagaaga tatagctgga gggcttatgg ttactatgcc ttcagaaaaa
     9241 gactttaaat cagaccttaa agttggaaca agtggaatga ctattggcga agtatgtaac
     9301 aagtatttta aagcttcttc agtggcttca acagaagaaa gaatgagaat attaagattc
     9361 ttagaaaata tatgtcttgg ttcatctgct gttggttata gaactgagtc aatgcatggg
     9421 gcaggttcac cacaagctca aagaataatg atttcaagac aaggaaacat aaatcaaaag
     9481 aaagaattag ctaagaaaat tgcgggaata aaaaaagagg aagctttaaa tttataaaaa
     9541 gttgatatat aaagggaaat tatatattaa tgtgtattaa ttaatgaaaa gatatgtagt
     9601 ataagagata gttataaaat tatggtggtt tttagctaag taaagaaatt tttaataagg
     9661 agaatttaca catgacctgt ggagttagat tttgtggggg ctgtaaccct agatacgaaa
     9721 gagggaaggc tcttacaata atcaaagagc acttcaatgg tagggtagat tttagcatag
     9781 cacaagagaa cgtcgagtat gatttgctac ttataattgg aggatgcgca actctatgcg
     9841 catcctatca acaatataaa gtgaaaaatg aatgtgttaa gatgtgggat tttagccata
     9901 tagaaagagt tataaatgtg attgaatcag caatttgtga aacttaaaaa agctaaatgg
     9961 ggggatttca ttgagttggc aagaattgta tcaaagtaaa ttatgtagtg ctacagaagc
    10021 tgtaaaacag attaaaaacg gtgatactgt agtatttgca cattgtgtag gtgaacctcc
    10081 tgcactagta gaggctatga ttgaaaatgc tgaacaatat aaagatgttg agattaaaca
    10141 tatggtaagc ttgggtagtg gtggatatac tgctaaggga atggaagctc attttagagt
    10201 aaatccaatg tttgtaagtg gaaatgtaag aaaggcaatt gaaaatggag atggtgattt
    10261 tacacctgca ttcttccatg aagtaccaaa gttgcttaga gaaaaaagat taaaatgtga
    10321 tgttgtttta gcacaggtaa ctccaccaga tgaacatggg tattgtagct taggaacatc
    10381 agttgattat acttatgaag ccattaaaac tgctagaaca gtaattgttc aagtgaatga
    10441 ccagtttcct agaacctatg gtgaggtagt ccatgtatca gagtttgact atatagtgga
    10501 aaaatcacaa cctttatttg aactacaacc tgcaaagatt ggagaagtag aagaagcaat
    10561 tggtaaaaat tgtgcttcat taattgaaga tggttctact ttacagctag gtattggtgg
    10621 gataccagat gcagttatgt tatttttaac tgataaaaag gatttaggga ttcattcaga
    10681 aatgatttca gatgggactt tggcccttta tgaaaaaggt gttataaatg gtaaatataa
    10741 aaattttgat aaagaaaaaa tgacagttac cttcttaatg ggtactaaaa aattatatga
    10801 ctttgcaaat aataaccctg cagtagaggt aaaaccagta gactatgtga atcatccagc
    10861 aattataatg aaacaacata agatggtttc tataaattct gctatacaag ttgatttaat
    10921 gggacaggta gttgcagagg ctatgggact tagacaattt agtggtgttg gaggtcaagt
    10981 tgactttata cgtggagtgt ctatggggga agatggaaag gcaattatag ctatgccttc
    11041 aataactaca aaaaaagatg gtacagtaat ttcaaaaatc gtatctattg tggatgaagg
    11101 tgcacctata acgacatcaa gaaatgatgt tgactatatt gtaacagaat acggtatagc
    11161 agaattaaaa ggaaaatctc ttagagaaag agcaagaaat ttaataaata tagctcatcc
    11221 aagtgtaaga gaaagtctag cagtagaatt tgaaaagaga tttaaggaga aatattaata
    11281 atagaggggt gttaataatg gaagcactta gagtagttcc aaagatattt tattttgata
    11341 catttaaaga atttaatgaa gaatttaaaa taggaaaaaa tgatttagta attactaatg
    11401 agtttatata tgagccatat atgaaacctc ttggaataga tacaaattta atttttcaag
    11461 aaaagtttgg aacaggtgaa ccatctgatg aaatgataga ctccatgaca aaggaaatga
    11521 agaaatacaa ttttgataga attattgcgt ttggaggagg aacaatagtt gatatatgta
    11581 agatattagc acttgatgtt ccagaaaagt ctattgattt atttgaagga gaagtatcac
    11641 ctaaaaaagt aaaagagtta gtagtagttc ctacaacatg tggtacaggt agtgaagtaa
    11701 caaatgtagc tattgcagag ttaaaatcta aacatactaa aaaggggctt gcagtagaag
    11761 aaacatatgc agactatgca gtgttaattc cagagacagt aaaagggtta ccttacaaat
    11821 tctttgttac aagttcggta gatgctctta tacatgctat tgagtcttat ctttctccaa
    11881 aagcaagtcc atttacagag atgtattctc tacaagcaat aaaaatgatt atggatgggt
    11941 ataagaagat agttgataaa ggagaagaag agagatttaa tcatcttaga gactttgtac
    12001 ttgcatcaaa ttatgcagga attgcttttg gaaatgctgg ttgtgcagca gttcatgcac
    12061 tatcttattc aataggtgga gctttccatg ttgcacatgg tgaagcaaat tatcagttct
    12121 ttactgaagt atttaagatg tattctagaa aaaaacctaa tggaaaaatt ataaaatgta
    12181 caaaaatttt agcagatgcc cttgaatgtg accctaactg tgatgtttat ggagaattag
    12241 aaaagttttt aaataaatta atagctaaaa aagctttgag agaatatgga atggtggaaa
    12301 gtcagataga tgaatttaca gatagtacaa tagcaaatca acaacgacta cttgctaata
    12361 attatgtaga gcttagtaga gaggaaataa gagagatatt tgcaaatctt tattaataca
    12421 attttttatt tatatgtata gtcaacataa acaatgctaa ttatatttat gtacaaataa
    12481 aaataaggga acttaaattt taattaaagt taagttctct tatttttata tggagttaaa
    12541 tataagtata tttttaattt aaaatatgct tatatttaag tctattactt tatttgaaac
    12601 aatttgtaga atttcaaatt ccttgacagt aataagtaag taattgtata atcaaactgc
    12661 aatatattaa taatattagt gaaaatgaaa ggattataaa atatgtataa atcagaaaat
    12721 ttttcaaaaa aatctgtatt aataaattca tttttaagta aatttttaaa aataattgga
    12781 tggggtttgg taagcttatt tacattatca gccttagtac tttcaactat ttccattata
    12841 attggatggg ctgtaatact tcctattcaa gtatgtgcag tatttttact tataggagga
    12901 gtttgtattt ttaaaggtaa gagattagga gaccaaattt ctcgttataa aaaatattgt
    12961 acaattataa acaatagaaa tattattcca gtagaactta ttgcagaaaa cacatctaaa
    13021 tcattaaatt ttataataaa agatgtacaa aagatgatag acaaaaacta ctttataaat
    13081 acttatattg ataaaagaaa taatcagatt gtgttaacaa atgaagattt tattccacca
    13141 agatatgaag atgtagcata tgaagtcaaa gaagaagaaa agactgatga atccaatgaa
    13201 gttgatacaa ttataaataa aggaatgaat tatctaacac aaataaaaga agctaataaa
    13261 aatattaaat cagaatcaat gggcgataaa attgtacaag tagaagatgt tacaagtaaa
    13321 atatttgatg ttgtaaaaca tgacccatca aaacttactc aaatacaaaa atttatggat
    13381 tattatcttc caaccacttt aaaactatta aattcatatc atacattgga agaacaagga
    13441 attgatagag aaaatataac aacaactatg gaaagtattg aaaatacaat gaatacgatt
    13501 gtagtagctt ttgaaaatca attggactat ctgtttgaag atgaagccat tgatatttct
    13561 acagatatta ctgttttaga gaatatgtta gtccaagaag gattaacgag tggtaaattt
    13621 taacttcaat agaaaaaata ttctgttatt tttaaacaat taattagtca taaagtataa
    13681 aatgtgtatt atataagtta atcagtactt tatgaaggaa gaggagagaa agatacaatg
    13741 aataatttag atgatatacc agttatgcca actttgacac ttgacccatt tggagaagaa
    13801 tcaaacacta atgatataga taattctgac ttattaatga aaaaggatga aaaagaccca
    13861 gaagaagaaa aactgtctga atctgaacgc aagatggtaa aggaatttgc ggaaaaaata
    13921 gatattacaa atacaaatat gattttgcaa tatggagctg gtgctcaaaa aaaggtagca
    13981 agtttttctg aaacagcttt aaagagtgta aaaacaagag atttaggaga agttggagat
    14041 atgcttacaa atctagttac tgatttaaaa gcattttcag cagatgaaaa agaacaaagt
    14101 ggttttttag gaatttttaa aaaggcaaat aataaaattt caaatttaaa aactaagtat
    14161 gatagtgcag aggtaaatgt ggataaagta agtaaagagc ttcaaaagca tcaggttaaa
    14221 atattgaaag atatagcaat gcttgataag atgtatgaat tgaacttagc ttactcaaaa
    14281 gagcttacta tgtatattat agcaggaaaa caaaaattaa aggatatgaa agaatatgaa
    14341 atgccaaagt tgagagaaaa agctcgttta tctggttcaa cagaagatgc tcaatctgta
    14401 aatgatatgg tttcactttg tgacagattt gaaaagaaaa ttcatgactt agaattaact
    14461 agaatggtat cgcttcaaat ggctcctcag ataagattgg ttcaaaatag taataatctg
    14521 atggcagaaa agattcaatc aactatagta aataccattc cattatggaa aaatcaaata
    14581 gtgcttgctc ttggaatttc tcacttaaat caagctatga aagcacagcg tgaagttagt
    14641 aatatgacaa atgagctttt attgaagaat gcagaaactt tgaagatggg aacaatagaa
    14701 acagcaaaag aatcagaaag aggaattgta gatatagaca caattaagaa aactaatcaa
    14761 tctctaatat ctactattga tgaggtagtt aaaatacaac atgatggtag aataaagaga
    14821 caagaagcag aagttgaatt atcaaaaata gaaaatgaac ttaaaagtaa gcttcttgaa
    14881 tttacagtta aataaaaatt atactaatat aaaaagagac tgtctcaaaa tagagataag
    14941 tctctttttc attgtttaaa tgattaaagt atatttatat tataaatttc tattatcttt
    15001 taacataaca tcatgagctc caccttcaac tatactagta gaagatacaa gtgtaagttt
    15061 agcatttttt tgaagttcag gaatgctaag tgctccacag ttacacatag tagaacgaac
    15121 tttacttaaa gaaagagtta cattgtcttt caaagaacca gcataaggca cataagagtc
    15181 aacaccttct tcaaaagaaa gtttcttatc tccaccaagg tcatatcttt gccagtttct
    15241 agcacgagca gaaccttctc cccaatactc tttcatataa ctaccattta tattaacttt
    15301 gttagttgga ctctcatcaa aacgagagaa gtatctacca agcattataa aatctgcacc
    15361 catagctaaa gctaaagtta tatgatggtc ataaacgata ccaccatcag agcaaattgg
    15421 aatataaata cctgtttttt caaagtattc gtctctagct tgagcaactt caattataga
    15481 agtagcttgt ccacgtccta tacctttttg ttcacgagtt atacatatag aaccaccacc
    15541 aataccaact ttaacgaagt cagcaccagc ttcagctagg taaaggaatc cctcgcggtc
    15601 aactacattt ccagctccta ccttaactgt atcaccatat ttttcacgaa tgaaatcaag
    15661 tgttattttt tgccactcag aaaaaccttc tgaagaatct atacataaaa catcagcacc
    15721 agcttcaact aaagcaggaa cacgttcagc aaagtctcta gtattaatac cagctccaac
    15781 tacatatctc ttagaagaat ctaaaagttc aagtggattt tctttatgag aagagtaatc
    15841 tttacggaat accatataca taagattacc attatcatca agtacaggaa gagaattaag
    15901 tttgtggtcc catataatgt tgtttgcttc ctttaaagta acatctttgt tagcataaac
    15961 tatagatgac ataggagtca taaattcact aactttagta tctaaatcca tacgacttat
    16021 acgataatca cgacttgcaa caatacccaa taatttacca gttgaagttc catcttcagt
    16081 aacagctact gtagaatgtc cagttttttc ttttaagtca agtatgtctt ttaatgtgtt
    16141 atcaggttta atatttgaat cacttacaac aaaaccagct ttgtgggatt taacacgtgc
    16201 taccatagca gcttcatttt caatagtttg agaaccgtat ataaatgaaa taccaccttc
    16261 tttagcaagt gcaatagcca ttttgtcatc agaaacagat tgcataatag cagaagtcaa
    16321 aggtatattc atataaatat ctgcttcctc gcccttttta aatttagtaa caggtgtttt
    16381 taaacttaca ttagcaggta tacaatcagt agaagagaaa cctggtacaa gaaggtattc
    16441 actgaaagta tgtgatggtg tatcaaaata aaacgccatt ttttaatcac tccttatatt
    16501 tttatacttt ttataattga tagtattaat tataacttat tctttaattt tattaaagta
    16561 ttttgtcaaa tattgagatt tttgaaaaat ttaaactatt ttaattaaat ttgtttaata
    16621 tatattgcct tatagggta
//
LOCUS       DAMJWP010000068        16492 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.070, whole genome shotgun sequence.
ACCESSION   DAMJWP010000068 DAMJWP010000000
VERSION     DAMJWP010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16492
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.070"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            64..726
                     /locus_tag="PC700_001829"
     CDS             64..726
                     /locus_tag="PC700_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581140.1"
                     /translation="MIDVSIDDKLKERWPNAKLGCIQAKVAVNKSSEKLINEINEFCD
                     TINQSLKVEDITKLDKIKDARNAYKELGKNPSRYRTSSEALVRRIIQGKGLYTINNIV
                     EINNLISIKSLYPVGTYNVSKLHSPVCFTVGDEGEQYKGIGKELINIENLPILSDSVG
                     KFGSPTSDSERAMITEDANEILICIFSFSQESDIEEYFGYAKELLKEYADGKDIETKI
                     IK"
     gene            1096..2505
                     /locus_tag="PC700_001830"
     CDS             1096..2505
                     /locus_tag="PC700_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HDF2581141.1"
                     /translation="MIDGKEVKQESGQKNLKFKIAIVVGVLFVAYIGMAIFFRNHFYF
                     GTTINGVKASGKTVEQVNDLLTSSTNSYTLNLEERNNKKEQIKAKDIDLKFTPDDKVQ
                     KIKDSQSAIGWIGGIFKSKEYNNMITLSYNKDLLEKRFNALSCFDSKNVVEPKSAYPK
                     YDSTKNAYVIVPEVLGNKVKKDDFYKLVQNSINSGNTSISLDESKVYEAPNNTKDSEK
                     LKQAIKTLDKYVGVVVTYDFSDRKETLDGSEINKWLKVDSEKNYDITFDNDAMVEYTR
                     SLSKKYSTFGDSRPFKTATGKDITISGGIYGWLIDKKKEAEALVDVVKAGKNVTREPI
                     YIQKAVSRNKDDIGNTYVEISLSGQHMWFFKNGEMLVSTDVVTGDLARGFATPSGIYP
                     LNYKARNVSLTGQGYSSPVSYWLPFNGNIGIHDATWRNSFGGGIYKSSGSHGCVNTPF
                     SKAKKIYENIEPGTPIILY"
     gene            2620..2895
                     /locus_tag="PC700_001831"
     CDS             2620..2895
                     /locus_tag="PC700_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HDF2581142.1"
                     /translation="MNEKLTKILEEAKNDDEFRKELIKTREQRDVMDSFCKVVTSRGY
                     DITVGELFSMGEEYTSNLLKSVNGGAAYPIEDWSDWYEDFFIMLSNI"
     gene            3525..3836
                     /locus_tag="PC700_001832"
     CDS             3525..3836
                     /locus_tag="PC700_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581143.1"
                     /translation="MAFEIIKSIVEAEQTADSIKVKAVTDAESIRADAVNKCESIFAD
                     VRKQAKLMEETLIEKAVTDSRAEVDKILANAKSECLKIEKTAEERKSKAIEAVIGKVV
                     R"
     gene            3838..5763
                     /locus_tag="PC700_001833"
     CDS             3838..5763
                     /locus_tag="PC700_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HDF2581144.1"
                     /translation="MAIVNMNKISIVGLEAQKSQILKLLMNRGFVQIDDSAFLTEEEE
                     LKNYLVKDSEESEVIMLEQKMSHVNQAINIVSSLVKQKKSLLAPKREFNKIDSEKEGQ
                     VYAEALELNNLHKEIGTIRSNINLLNNNKSMLEPWVNFDIPLENMETKYTKTILGTVP
                     ISANIDALRVKLEDEIPESVIGIVNSDKLMNYVYLITLKETFDDVLEVLKEFNFSVVS
                     LPNEEGTPLQAIKKYNEMIIRQENVSKDKTEEIKRHADSIHMLENLYDFYTIERDQKK
                     IIERLVKTKTTFCLNGWLPSKRADELIKEITEKFDCCVQTEEGSKEEGFPILLENNKL
                     VTPFESVTNMYSYPSTKDIDPNTILTFFFVVFFGMMLSDAAYGLIIAVVCGFVVYKLK
                     IQKGEGNLIKLIGICGVSTTVWGLIFGGILGDLIPIKALINPLEDVMTLMGMSLLFGI
                     IHIYVGLGIKAYTLIRSGKVIDAIFDIGFWYLCITGVCLLIIPFVAGDIGVFSEVGKY
                     LAIIGAIGLVLTQGRSFKGIPVKLFKGFSSLYGITSYFADILSYTRIMALCLTTGVIA
                     QVINLLGAIAGPILAVVIGVVGHTINLLINALGAYVHTSRLQYVEFFNKFYEGGGVPF
                     VPFKYKTKYTSINKKEM"
     gene            5766..6257
                     /locus_tag="PC700_001834"
     CDS             5766..6257
                     /locus_tag="PC700_001834"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HDF2581145.1"
                     /translation="MEFLNALGNGQFLAISGAAIAALFAGMGSAKGTSIAGQAAAGVV
                     TEDPSKFGQLLVLQLLPGTQGLYGFIIAFLILSKAGIIGGDAIPTSAQGLQLLMAGLP
                     IGLVGLVSGIAQGKAAAAGVGIVAKRPEELGKAITFAAIVETYALLGLLVSFLAYNGI
                     AIG"
     gene            6309..6872
                     /locus_tag="PC700_001835"
     CDS             6309..6872
                     /locus_tag="PC700_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HDF2581146.1"
                     /translation="MGNEQKMIDRIIADAKQEAQEILDKAKSEADLKVNSANEKAEKE
                     MASYTKLAEAEAEKAASKEISGAYMEAKKQILSKKQEILEEVILEAKNKLLNLKDNEY
                     EEIILNMIEKSNCTDDSEIVLSKKDKKTLKDVLSKKGIKVSDETRDITGGFIVKKGDI
                     EYNYSFEAIIAVEHEYIEQIAAEILFN"
     gene            6887..7864
                     /locus_tag="PC700_001836"
     CDS             6887..7864
                     /locus_tag="PC700_001836"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HDF2581147.1"
                     /translation="MAEEKTYPYAVARIRVLEKQLLNRQMFIQMAEAKSPEDSLRIIA
                     EAGYADTSNNNIHNFENILTQELSKTYEILKSLAPEEKFVDVFLYKNDYHNLKVLIKE
                     EISGVDGEKYLIDGGTIPLIKLRESLANRSFSDLPKIMSSAILEAFDVYNKTQNGQMV
                     DIVLDKATFTSMKETAKESQNKFVIDYVMKVCDLTNLKSFIRVKNMKKDFDMFMNVFV
                     SGGSLDKEKFLEAFSSDTPASCFKSTSYSDVCKNGMDSGFTVFEKLCDDYLMEYVRGA
                     KFKPLTLEPLIAYLYAKESEIKTIRIILTSKLNNIDADTIKERLRDAYV"
     gene            7851..8174
                     /locus_tag="PC700_001837"
     CDS             7851..8174
                     /locus_tag="PC700_001837"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HDF2581148.1"
                     /translation="MLMYKVGVVGDKDSIMGFLALGIDIFPAYDSDEIKKSIHKLVED
                     EYAIIYITEQASLLAKESIAKYKDYQLPAIIVIPGIGGSMGLGMNEVRESSKRAIGAD
                     ILFKE"
     gene            8291..10069
                     /locus_tag="PC700_001838"
     CDS             8291..10069
                     /locus_tag="PC700_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HDF2581149.1"
                     /translation="MKTGTIVKVSGPLVVAEGMRDANMFDVVRVSDKHLIGEIIEMHG
                     DKASIQVYEETSGLGPGEEVVSLGMPMSVELGPGLISTIYDGIQRPLEKMYDISGTNI
                     TKGVEVSSLDRTTKWEFKPKKSVGDKVVAGDIIGGVQETAVVECKIMVPYGVKGTIKE
                     IYSGEFTVEDTVCVITDEKGNDIPVTMMQKWPVRKERPYREKKTPDSLLVTGQRVIDT
                     FFPITKGGVAAIPGPFGSGKTVTQHQLAKWADADIVVYIGCGERGNEMTDVLLEFPEL
                     KDPRTGESLMQRTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYSVALMADSTSRW
                     AEALREMSGRLEEMPGEEGYPAYLGSRLAQFYERAGKVNCLGMDEREGTLTAIGAVSP
                     PGGDTSEPVSQATLRIVKVFWGLDASLAYKRHFPAINWLTSYSLYQEKVDVWADKNVN
                     DNFSAQRAQAMKLLQVESELQEIVQLVGVDSLSDGDRLILEVTRSIREDFLHQNSFHE
                     VDTYTSLHKQYRMMGLILKFYKSAQDAIKQNVTINDIIKLSVLEKIGRAKYVEEGDID
                     AAYDSIEDEIDNELADLIKKRGAEEL"
     gene            10066..11439
                     /locus_tag="PC700_001839"
     CDS             10066..11439
                     /locus_tag="PC700_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HDF2581150.1"
                     /translation="MIKEYKSIQEVAGPLMLINGVEGVKFDELGEIELSNGEIRRCKV
                     LEVNGDTALVQLFESSTGINLAESKVRFLGKSLDFGVSPDILGRVFSGMGRPIDGGPE
                     IIPDKRLDINGAPINPAARDYPAEFIQTGVSAIDGLNTLVRGQKLPIFSGSGLPHANL
                     AVQIARQAKVRGTDSKFAVVFAAVGITFEDAEFFISDFKATGAIDRSVVFVNLANDPA
                     VERVSTPRMALTAAEYLAFEQDMHVLVIITDITNYAEALREVSAAKKEVPGRRGYPGY
                     LYTDLATMYERAGKMKGHEGSITMIPILTMPEDDKTHPIPDLTGYITEGQIILSRELY
                     KKDIQPPIDVLPSLSRLKDKGIGKGKTREDHADTMNQLFAAYSRGKDAKELMAILGES
                     ALSEIDLMYAKFADEFEKEYVSQGFRTDRTIEQTLEIGWKLLSMLPKSELKRIRDEYF
                     EKYMPKE"
     gene            11445..12113
                     /locus_tag="PC700_001840"
     CDS             11445..12113
                     /locus_tag="PC700_001840"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HDF2581151.1"
                     /translation="MARLNINPTRMEMTRLKKLLKTATRGHKLLKDKLDELMKQFLEI
                     VRENKRLREEAENALDTAYKNFIIARAVMSQEYLGSALMMPKQSVSVDVSTRNIMSVD
                     VPVFDFKTENNQSDIYPYGLAFTSGELDSAMEAFSDAMQPLLRLAESEKSAQLLAQEI
                     EKTRRRVNALENVMIPNYIETIKYIAMKLEENERASTTRLMKVKDMVLKKALEEKKKN
                     DLVV"
     gene            12390..13439
                     /locus_tag="PC700_001841"
     CDS             12390..13439
                     /locus_tag="PC700_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2581152.1"
                     /translation="MKEFIFTCLCIVVITFMTSCISTTPQTTQGKGNNKLTIYAALEE
                     EQIAYYMEGFREEYPNIDVEIVMDSHGVIASKLLAEKDNPQADIIWGLSAMNMIELKK
                     DGALEAYKPKNYDKVDKKFKDTEDDVSWVGMSVPETAIVVNYKELKKLGIDIPKSYED
                     LIKPEYKGLITMPNPASSGTGYLTVSGLIQMMGEKDAYNYMDKLHKNIATYTHSGSKP
                     AKLAGSGEYPIGITLGYRATQQLSSGEPIEVVFPKEGSGWDLEANALVKKDNIKKEAK
                     LFLDWAISDSAMDRYAHEVAVTSIKTYNPVPKGYSKQPFEQLIKEVDLNSAAKNRSTV
                     LKTWESKYGSKSESK"
     gene            13535..14524
                     /locus_tag="PC700_001842"
     CDS             13535..14524
                     /locus_tag="PC700_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2581153.1"
                     /translation="MSYLKINNVFKSYDQKRVLNNISLDIEEGEFLCLLGPSGCGKTT
                     LLRIIAGLEDVNSGTIILQDKDITNLEPSKRGFGIVFQSYALFPNMTAYNNIAFPLKE
                     RKVSKEKIDNKVKEVLETVGLTNEAHKYPKALSGGQQQRIAIARALALEPKFLLLDEP
                     MSALDAKVRHKLRMDIKRLQKELNITTIMVTHDQEEAITMADKIAILNGGDIMQIGTP
                     EEIYQNPQNLFTAQFIGDTNCFDNGDSILTVRPEYVQIEKSTKENYQGIISNIEFRGN
                     LLRVEIKDKLNENFIISDVSIKEWVNLNLVEGDLVKISIDEKYYLKYPKVKGA"
     gene            14529..16247
                     /locus_tag="PC700_001843"
     CDS             14529..16247
                     /locus_tag="PC700_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HDF2581154.1"
                     /translation="MNNNAISSKSISLKSKMNLDIKKCMLTVLVMSLVIFLLAPIIYL
                     FVQAFLDKDGVFVGLQNFQEYLASESLLNSLKNTVFVSVISTVISLTLALVYAYALTR
                     TKIKFKGFFKSLAMFPMFAPTMMHAIGLVYLFGNQGLVTNLLGVHVPIYGAVGIILAQ
                     IIYVFPQSFQILYISLSSTDYRLYEASNTMGISKIREFISITIPSIKYSLISSAFVSF
                     TLCFTDFGAPKVIGGEFNVLSIDVYKQIIGQQNMPMGATVSILLLIPAIISFIVTKKI
                     EKNQNSYITSKSIPYKVKTNKLRDSILGGVVGIIAFGIIAVMLTVILSSLISVWPYDL
                     RLTLEHFSMKNSIDGIKPFINSIKMSLLTAILGTSFVFLFAYLNEKTEKAPYLKSFGY
                     FLSTLPMALPGLVLGIAYVLFFNKLEFNFMDTIYIKNLFNGLYGTLLIMVICNIVHFF
                     SVAFITATTAIKKVDKELDMVAKSMGINSASILKNVTIPLCLPAVLENFMYFFLNSMV
                     TISALVFIYTASSKVAAISIVQLDDKGSTAQAAALAVLILVTNILVKVIYEFIKNRLE
                     KRTNFN"
     gene            complement(16337..>16492)
                     /locus_tag="PC700_001844"
     CDS             complement(16337..>16492)
                     /locus_tag="PC700_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2581155.1"
                     /translation="LKIVEENSTNELKLSMSIGYAIFDEKVDVSLFDTYRRADSQMYD
                     NKKKINK"
BASE COUNT         6342 a         2010 c         3078 g         5062 t
ORIGIN      
        1 gtactataat gtatagatga taaaaacttt aataaaaatt tagtgtatag gagttgttta
       61 agtatgatag atgtgagtat tgatgacaaa cttaaggaaa gatggcctaa tgctaagttg
      121 ggttgcattc aagcaaaagt agcagtaaat aaaagctcag aaaaattaat taatgaaata
      181 aatgagtttt gtgatactat aaatcaaagt ctaaaagttg aagacattac gaaactagat
      241 aaaattaaag atgcaagaaa tgcttataaa gagcttggaa aaaatccaag tagatataga
      301 acatcttcag aagcattggt tagaagaatt attcaaggga agggtcttta tacaataaat
      361 aatatagtag aaataaacaa tttaatatca ataaaatcat tatatccagt aggtacatat
      421 aatgtaagca aattacattc accagtatgt tttactgtag gagatgaagg agaacagtat
      481 aagggtattg ggaaagaatt aataaatata gaaaatcttc ctatattgtc tgattcagtg
      541 ggtaaatttg ggagtccaac tagtgattct gaaagagcaa tgataacaga agatgcaaat
      601 gaaatattaa tttgtatatt ttcttttagt caagaatctg atattgaaga atattttgga
      661 tatgctaagg agttacttaa agaatatgct gatggaaaag atatagaaac taaaataatt
      721 aaatagaatc tatataacaa cgttatgtta agctaaaatt caatataaaa ataagttaag
      781 ataaagaaac taacataaaa ttagtttctt ttttaaaaga gtagtttagt gttacaaaaa
      841 cagatagata acccacgatt attaaaagta tggtaatata ttaatatagc agagattgta
      901 tttaagggat tttgaaaggg gtgatttata atagtcattt tgtctaaaag ataaaatggt
      961 tttatggaaa taactatata tttaaaaata taaacaatga tacagtaatt tgggtatata
     1021 aaataaggca tacatggttt tgtttaaaaa ccaataaagt acataggtaa ttacaatgta
     1081 aggaaggatt taattatgat tgatggaaaa gaggtaaaac aagaaagtgg acaaaaaaat
     1141 ctaaaattta agatagctat agttgtaggt gttttgtttg tagcttatat aggaatggca
     1201 atctttttta gaaatcattt ttactttgga acgactatta atggcgtaaa ggcttcagga
     1261 aaaacagttg agcaagtaaa tgatttgtta acatctagta ctaattccta tactttaaac
     1321 ttagaggaaa gaaacaataa gaaagaacaa ataaaagcaa aagatattga tttaaaattt
     1381 acaccagatg ataaagttca aaagataaaa gatagtcaaa gcgctattgg atggattggt
     1441 ggaatattta aatctaaaga gtataataat atgataactc tttcatataa taaagattta
     1501 ctagaaaaaa ggtttaatgc tttatcttgt tttgatagta aaaatgttgt agaacctaag
     1561 agtgcttatc ctaagtatga tagcactaaa aacgcttatg ttatagtacc agaggtactt
     1621 ggaaacaaag tgaaaaaaga tgatttttat aagttagtac aaaactcaat taattctggg
     1681 aatacttcta tttctctgga tgaaagtaaa gtttatgaag caccaaacaa cacaaaggat
     1741 tcagaaaagt tgaaacaagc tattaagaca ttagataagt atgttggtgt agtcgttaca
     1801 tatgatttta gtgaccgtaa ggaaacttta gatggttctg aaattaataa atggttaaaa
     1861 gtagactcag aaaagaatta tgacataact tttgataatg atgctatggt agaatataca
     1921 agaagtttaa gtaaaaaata cagtactttt ggtgactcaa gaccatttaa aactgccaca
     1981 ggtaaagata ttacaatttc aggtggaatt tatggttggt tgatagataa aaagaaagaa
     2041 gctgaagcat tagttgatgt tgtaaaagct ggtaaaaatg ttacaagaga acctatttac
     2101 atacaaaaag ctgtatctcg taataaagat gatattggaa atacatatgt agagattagt
     2161 ctttctggcc aacatatgtg gttctttaaa aatggagaaa tgcttgtatc aacagatgtt
     2221 gtaacaggcg atttagctag aggatttgct acaccatcag gtatatatcc tcttaattat
     2281 aaggctagaa atgtttcatt aactggtcaa ggttacagtt cacctgtaag ttattggctt
     2341 ccatttaatg ggaacatagg cattcatgat gctacttgga gaaattcttt tggtggaggt
     2401 atatataagt cttcaggttc acatggatgt gtaaatacac ctttttctaa ggcaaagaaa
     2461 atatatgaaa atatagaacc aggtacacca attattttat actagtaaat gtgttgctaa
     2521 cagttaagcg aagtcatctt agtttattta ggatggcttc ctttttatat aaagcttatt
     2581 gaagtttaca gaaagactac ttaaaaggag aaatacataa tgaatgaaaa attaactaaa
     2641 attttagaag aagctaagaa tgatgatgaa tttagaaagg aacttataaa aacaagagaa
     2701 caacgtgatg ttatggattc attttgtaag gttgttacat ctagaggtta tgatataaca
     2761 gtaggggaac ttttttccat gggagaagaa tatacatcaa acctactgaa aagtgtaaat
     2821 ggaggagcag catatcctat tgaagattgg agtgattggt acgaggattt ttttataatg
     2881 ttgtcgaata tatagagtaa ctaatgatat tatctactta agattaatac attgccttaa
     2941 aataaaacta ttagaactaa gtgtaagttt tatcaatctt taaaaatatt gatttatttc
     3001 taactatata taaaaaattt agattgaaaa taaggtgaca aaaatcgata tagcttgatg
     3061 atttataaaa gttagataaa atagtgcgag atatttctaa aaattcaaaa aaaacaataa
     3121 acttcaaggg ttagaggttg aaaaagaata taaaaaactt ttatatgtaa taaattaaat
     3181 taaaattata aaaaaaataa aataaaaatt taaaatgtcg acatagcgtt aattttaaaa
     3241 caaaataacg ctattgacaa actatatcgc tttaaattat aattattgaa aataatggta
     3301 tttaaaaacc atactcatgt catagtctaa aagtaagtca aaaaaggatg taaatctagg
     3361 caagtactat tctaaaatat gttttattaa gaaataaacg ttgaaaaata ttaattatta
     3421 tataaaaatg ctgtttatac tttaacaatg aggtagtaat ctaattagaa taaaaaaatc
     3481 taattggtta tatacatagt aaaaaggtaa gagaggtgat ttggatggct tttgaaataa
     3541 taaaaagcat tgttgaggca gagcagacag cagacagtat caaagtaaaa gctgttactg
     3601 atgcagagtc tatcagagct gatgctgtaa acaaatgtga aagcatattt gctgatgtaa
     3661 gaaaacaagc aaagcttatg gaagaaactc ttattgagaa ggcagtcacc gacagtagag
     3721 cagaggttga taaaatctta gctaatgcta aaagtgaatg tctgaaaatt gaaaaaactg
     3781 ctgaagaaag aaaaagtaag gctattgaag cggttattgg aaaggtagtg agataatatg
     3841 gcaatagtta atatgaacaa aatatctatt gttggcttag aagctcaaaa aagccagatt
     3901 ttaaagctac ttatgaaccg tgggtttgtt cagattgatg atagtgcctt tcttactgag
     3961 gaggaggaat taaaaaatta tcttgtaaaa gatagtgaag aatctgaagt tataatgcta
     4021 gagcagaaaa tgtctcatgt aaaccaagca attaatattg tttcttcact agtgaaacaa
     4081 aaaaaatccc tacttgcacc aaaaagagag tttaacaaaa ttgatagtga aaaagaaggg
     4141 caagtttatg ctgaagctct ggagttaaac aatttgcaca aggagatagg tacaataagg
     4201 tctaacataa acttacttaa caacaataaa agtatgttag aaccatgggt gaactttgat
     4261 attcctcttg aaaatatgga aactaagtat acaaaaacta tacttggaac tgtgcccata
     4321 agcgcaaata ttgatgcttt aagagtaaaa ttagaagatg aaataccaga aagtgttatt
     4381 gggattgtca attctgacaa gttaatgaac tatgtttatt taattacttt aaaagaaact
     4441 tttgatgatg ttttagaagt tttaaaagaa tttaattttt cagtggtttc attaccaaat
     4501 gaagaaggta caccgttaca agctatcaag aaatacaatg aaatgataat taggcaggaa
     4561 aacgtttcta aggacaaaac agaggaaatt aaaagacatg cagatagtat acacatgctt
     4621 gaaaacctat atgattttta cactatagaa agagaccaaa aaaagattat agagagactt
     4681 gtcaaaacaa aaacaacatt ctgtcttaat ggttggttac caagcaaaag ggcagatgaa
     4741 ttaattaaag aaattacaga aaagtttgat tgttgtgttc agactgaaga aggaagtaaa
     4801 gaagaaggct tcccaattct gcttgaaaac aataaattag taacaccttt tgaaagtgta
     4861 actaacatgt acagctatcc aagtacaaaa gatattgacc caaatacaat tttaactttc
     4921 ttctttgttg tattctttgg aatgatgcta agtgacgccg catatgggtt aataattgca
     4981 gtagtctgtg gatttgttgt atataaatta aagatacaaa aaggtgaagg aaatttaatt
     5041 aaacttattg gtatctgtgg agtttcaact actgtatggg gtcttatatt cgggggtatt
     5101 ttgggggatt taattcctat aaaagcatta attaatcctt tagaggatgt tatgacactt
     5161 atgggaatgt cgttactgtt cggtattatc catatatatg taggtttggg aataaaagca
     5221 tatacattaa taagaagtgg aaaggtaata gatgcaatct ttgacatagg tttttggtat
     5281 ttgtgtataa caggtgtatg tctacttata ataccttttg ttgcagggga cataggtgta
     5341 ttttcagaag taggaaaata tttagcaatt ataggtgcta taggtcttgt acttactcaa
     5401 ggaagaagct ttaaaggtat tcctgttaag ctttttaaag gattttcaag tctctatggc
     5461 ataacaagtt attttgctga tatattatct tatacaagaa ttatggcgct ttgtttaaca
     5521 acaggagtta tagcacaagt tatcaacctt ttaggggcta tagctggtcc gatacttgca
     5581 gttgtaatcg gtgttgtagg acacacaata aaccttctta taaatgcact aggtgcatat
     5641 gttcatacaa gtaggttaca gtatgtagaa tttttcaata agttttatga aggcggagga
     5701 gttccttttg ttccgtttaa gtataaaaca aaatatacaa gcattaataa aaaggagatg
     5761 taaatatgga atttttaaac gcgttaggaa atggtcaatt tttggcaata tcaggagctg
     5821 ctatagcagc attatttgca ggtatgggtt ctgctaaagg tacttcaata gcaggtcaag
     5881 ccgcagcagg tgttgtaaca gaagacccta gtaaattcgg tcaacttcta gttttacaac
     5941 ttcttcctgg tacacaaggt ctgtatggat ttatcatagc tttccttatt ttatcaaaag
     6001 cagggattat tggtggagat gcaataccaa catcagctca aggtttacaa cttcttatgg
     6061 caggacttcc aattggttta gttggtttag tatcaggtat agcacaaggt aaagcagcag
     6121 cagcaggagt tggaatagtt gcaaaaagac ctgaagaact tggtaaagct ataacatttg
     6181 cagcaatagt tgaaacatat gctcttttag gactacttgt ttctttctta gcttacaatg
     6241 gaattgcaat tggttaatta taaattataa ttcataagta aaaaaaatga aaaggaggaa
     6301 gcactcctat gggtaatgaa caaaaaatga ttgacagaat tatagctgat gccaaacaag
     6361 aagcacaaga aatattggat aaagctaaaa gtgaagcaga tttaaaagta aattcagcta
     6421 atgaaaaagc tgaaaaggaa atggcttcct atacaaagct tgcagaggca gaagcagaaa
     6481 aagcggcttc taaggaaata tcaggagctt atatggaggc taaaaagcaa atattatcaa
     6541 aaaaacaaga gattttagaa gaggtaattt tagaagctaa aaacaaactt ttaaatctta
     6601 aagataatga atatgaagag attatactaa acatgattga aaaatcaaat tgtacagatg
     6661 attcagagat agtattatct aaaaaagata aaaaaacatt aaaagatgtg ctatcaaaaa
     6721 aaggcattaa agtgtctgat gaaactagag atattactgg aggtttcata gttaaaaaag
     6781 gtgacattga gtacaattat tcatttgaag ccataattgc agtagagcat gaatatattg
     6841 aacaaattgc agcagaaatt ttgtttaatt aaggaggggt taaaaaatgg cagaagaaaa
     6901 aacttatccc tatgccgttg cacgaataag agtgcttgaa aaacagcttc ttaacagaca
     6961 aatgtttatt caaatggcag aagccaaaag ccctgaagac tctttaagaa ttatagctga
     7021 ggcaggttat gctgatacat caaataataa tatacataat tttgagaata ttttgactca
     7081 agaattatca aagacatatg aaattcttaa aagtctagct cctgaagaaa aatttgttga
     7141 tgtatttctt tataaaaatg actatcacaa tttaaaagtg ctgattaaag aagaaatttc
     7201 tggtgtagat ggtgagaaat atttaattga tggaggtaca attcctctta ttaagcttag
     7261 agaatcttta gcaaatcgta gttttagtga tttacctaag ataatgtcat cagctatatt
     7321 agaggcattt gatgtttata ataagacaca aaatggccag atggttgata ttgttctaga
     7381 taaagcaacc tttaccagta tgaaagaaac tgctaaagaa agccaaaaca aatttgtaat
     7441 agattatgtt atgaaagttt gcgatttaac aaatctaaag agttttataa gagtcaaaaa
     7501 tatgaagaaa gattttgata tgtttatgaa tgtgtttgtt tcaggtggct cacttgataa
     7561 ggaaaaattc ttagaagctt tttcctcaga tactccagca tcatgtttta aatctacttc
     7621 ttactctgat gtctgtaaaa atggcatgga tagtggtttt actgtctttg aaaaactttg
     7681 tgatgactat cttatggaat atgttagagg agcaaaattt aagcctctta cattagaacc
     7741 actaattgca tatttatatg ctaaagaatc agagataaaa acaattagaa ttattttgac
     7801 tagtaagctt aataatatag acgcagatac tataaaagaa aggctaaggg atgcttatgt
     7861 ataaggtagg agtagttgga gataaagata gcatcatggg atttttagct cttggaatag
     7921 atatcttccc agcctatgat agtgacgaaa ttaaaaaaag tatacataag ttggttgaag
     7981 atgaatatgc gataatttat attacagaac aggcatcatt gcttgcaaaa gaatcaatag
     8041 caaaatacaa agattaccaa cttcctgcca ttatagttat tccaggtatt ggaggaagta
     8101 tgggattagg tatgaatgaa gtaagagaat ctagtaaacg tgctataggt gcagatatat
     8161 tatttaaaga atagatgaaa gttgaataaa agacaatctc aaattttatt ggtgctaaag
     8221 atatgtgcta ataaaatata ggagggaata tttaaatttt gataaagtat atgattgcag
     8281 gtgagagaat atgaaaacag gcacaattgt aaaggtgtca ggaccgcttg ttgtagcgga
     8341 gggcatgaga gatgcgaata tgtttgacgt cgtaagagtt tcagataaac atcttatagg
     8401 cgagattata gaaatgcatg gagataaagc ttctatacag gtttatgagg aaacttccgg
     8461 tctaggacca ggggaagaag tagtttctct tggaatgcct atgagtgttg aacttggacc
     8521 aggtcttatt tcaactattt atgatggtat tcaacgtcca cttgaaaaaa tgtatgatat
     8581 ttcaggtact aatataacta aaggtgttga agtatcttca cttgatagaa caactaagtg
     8641 ggaatttaaa cctaaaaaat ctgttggtga taaggttgta gcaggagata taattggtgg
     8701 cgttcaagaa acagcagtag tagaatgtaa aattatggtt ccttatggag ttaagggtac
     8761 tataaaagaa atatattctg gtgaatttac tgttgaagat acagtttgtg ttattacaga
     8821 tgaaaaaggt aatgatatac ctgtaactat gatgcaaaaa tggccagtta gaaaagaaag
     8881 accatataga gaaaagaaaa caccagattc acttcttgtt acaggtcaaa gagttataga
     8941 tacatttttc ccaatcacaa aaggtggggt agcagctata ccgggacctt ttggaagtgg
     9001 aaaaactgtt acacagcatc agcttgctaa atgggcagat gcagatatag ttgtatatat
     9061 aggctgtggc gagcgtggaa atgaaatgac agatgttctt cttgaatttc ctgaattaaa
     9121 agacccaaga acaggcgagt cacttatgca aagaactgtg cttatagcaa atacatcaga
     9181 tatgccagtt gctgcacgtg aagcttctat atacactggt attacaatag ctgaatattt
     9241 tagagatatg ggatatagtg ttgcacttat ggcagactct acatcaagat gggctgaggc
     9301 tcttagagag atgagtggtc gtttagagga gatgcctggt gaagaaggtt atcctgcata
     9361 cttaggttca cgtcttgctc aattctatga gagagcagga aaggtaaatt gtctaggtat
     9421 ggatgaaaga gaaggaacac ttacagcaat tggtgcagtt tctcctcctg gtggtgatac
     9481 atcagaacct gtcagccaag caacgcttcg tatagtaaaa gtattctggg gtcttgatgc
     9541 atctttagca tataaacgtc atttcccagc aattaactgg ttgactagtt attcactcta
     9601 tcaggaaaaa gttgatgtat gggcagataa aaatgtaaat gataatttct cagctcaaag
     9661 agctcaagct atgaaacttt tacaagttga atctgaactt caagaaatag ttcaattggt
     9721 tggggttgat tcactttcag atggagatag attgatacta gaggttactc gttctataag
     9781 agaagacttc cttcatcaaa actctttcca tgaagttgat acttatacat cacttcataa
     9841 acaatataga atgatgggac ttatattaaa attctataag tcagcacaag atgctatcaa
     9901 gcaaaatgtt actataaacg atattatcaa gctttcagtt cttgaaaaga ttggtcgtgc
     9961 taagtatgta gaagaaggcg atatagatgc agcttatgat tcaattgaag atgaaataga
    10021 caacgagctt gcagacctta ttaaaaagag gggggctgaa gaactatgat aaaggaatat
    10081 aagtcaatac aagaggtagc cggtcctctt atgttaataa acggcgtaga aggtgtaaaa
    10141 tttgatgaac ttggagaaat tgaactttca aatggagaaa ttcgccgttg caaagttcta
    10201 gaggtaaatg gagatactgc acttgttcag cttttcgaaa gctcaacagg tataaacctt
    10261 gctgaaagta aagttcgttt tttaggaaag agtcttgact ttggagtttc accagatata
    10321 ttaggccgtg tttttagtgg aatgggtaga cctattgatg gaggtccaga aattatacca
    10381 gataaaagac ttgatataaa tggagctcca ataaatccag cagcacgtga ttatccggcc
    10441 gaatttatac aaactggagt ctctgctata gatggactta atacattggt tagagggcaa
    10501 aaacttccta tattctcagg gtctggtttg ccacatgcca atcttgcagt tcagattgca
    10561 agacaggcaa aagtacgtgg aactgattct aagttcgccg ttgtatttgc tgcagttggt
    10621 ataacttttg aagatgctga attttttata agcgatttta aagcaactgg tgcaatcgac
    10681 cgttctgtag tgtttgtaaa ccttgcaaat gacccagctg tagagcgtgt atctacacct
    10741 agaatggcac ttacagcagc agaatacctt gcttttgagc aagatatgca tgtacttgtt
    10801 ataataacgg atattacaaa ctatgcagag gcattgcgtg aagtttcagc agctaaaaaa
    10861 gaagttccag gacgtcgtgg atatccagga tatctttata ctgaccttgc tactatgtat
    10921 gaaagagcag gaaaaatgaa agggcatgaa gggtctataa ctatgattcc aatacttaca
    10981 atgcctgagg atgataagac acatccaatt cctgacctta caggatacat tacagaaggg
    11041 caaattatac ttagccgtga actttataaa aaagatatac agcctcctat agatgtttta
    11101 ccatcacttt ctcgtctaaa agataaaggt attggtaaag gtaagactcg tgaagaccat
    11161 gcagatacaa tgaaccagct ttttgcagct tattcaagag gtaaggatgc aaaagaactt
    11221 atggctatac ttggtgaatc tgcactttct gagattgatt taatgtatgc taagtttgca
    11281 gatgaatttg agaaagaata tgtttcacaa ggatttagaa ctgatagaac tattgagcag
    11341 acattagaaa tcggatggaa gcttcttagt atgttaccaa aaagtgaact taaacgtatc
    11401 cgtgatgaat attttgaaaa atatatgcct aaggagtgat atctgtggct agattaaata
    11461 taaatccaac acgtatggaa atgactagac ttaagaaact ccttaaaact gctacaagag
    11521 gtcataagct tcttaaagat aaacttgatg aacttatgaa acaatttctt gagattgtaa
    11581 gagaaaataa gcgattgcgt gaagaagcag aaaatgctct tgatactgct tataaaaact
    11641 ttataatcgc aagagcagtt atgagtcagg agtatctggg ttcagcactt atgatgccaa
    11701 agcaatcagt ttcagtagat gtttctacga gaaatataat gagtgttgat gttcctgtgt
    11761 ttgactttaa aactgaaaac aatcaaagtg atatttatcc ttatggtctt gcttttacat
    11821 caggtgaatt agattctgct atggaagctt tctcagatgc tatgcaacca cttttaagac
    11881 ttgcagaaag tgaaaaaagt gcacagcttc ttgcacaaga gatagaaaag acacgtagac
    11941 gtgttaatgc tcttgaaaat gtaatgatac caaattacat tgagactatt aagtatatag
    12001 ctatgaagct tgaagaaaat gagagagcaa gtactactag acttatgaag gttaaggata
    12061 tggtgcttaa aaaagcactt gaagaaaaga aaaaaaatga tttggttgta taattaaata
    12121 cttataaaaa taagtctaac attctacttt atagaggtta gacttatttt ttatgtttaa
    12181 attaaaatgt agatactata aaaattaata tattgtttaa aaacagactg tatttaacta
    12241 aaaataatat atttaacaaa aatttctaaa aattaacgaa ctgttaattt acacttaact
    12301 ttatattaac aaattaggtg tattatataa acataagcaa aaacaataaa aaacaaataa
    12361 aaaaaggaaa aaacacaaag ggggaagata tgaaagaatt tatatttact tgtctgtgca
    12421 ttgtagtgat tacatttatg acaagttgca tatcaacaac accacaaact actcaaggaa
    12481 aaggaaataa taaacttaca atatatgcag cactagagga agagcagata gcttattata
    12541 tggaaggttt tagagaagag tatcccaata tagatgtaga gatagttatg gattctcatg
    12601 gtgtaatagc ttcaaaacta ttagcagaaa aagataatcc acaagcagat attatatggg
    12661 gattatcagc aatgaacatg atagagctaa aaaaagatgg tgcacttgaa gcatataagc
    12721 ctaaaaatta tgataaagtt gataaaaaat ttaaagatac tgaagatgat gtatcttggg
    12781 taggaatgag tgtcccagag acagcaatag tagttaatta taaggaactt aagaaactag
    12841 gtatagatat tccaaaaagt tatgaagatt taataaaacc agaatataaa ggacttataa
    12901 caatgccaaa tccagcatca tctggaacgg ggtatcttac agtatcaggg cttattcaaa
    12961 tgatgggtga aaaagatgct tacaattaca tggataaatt acataaaaat atagctacat
    13021 atacacactc aggttcaaaa cctgcgaagt tagctggctc tggagagtat ccaataggta
    13081 ttacacttgg atatagagct acacaacaat tatctagtgg agaacctata gaggttgtat
    13141 ttccaaagga aggttcaggt tgggacttag aagcaaatgc cttagttaaa aaagataata
    13201 taaaaaaaga agcaaagcta tttttagatt gggcaataag tgattcagct atggatagat
    13261 atgcacatga agtagcagtt acttccataa agacatataa tccagttcca aaaggttatt
    13321 ctaaacaacc atttgaacag cttataaaag aagttgattt aaactctgct gccaaaaaca
    13381 gaagtacagt acttaaaact tgggaatcaa aatacggttc aaaatctgaa agtaagtaag
    13441 gtttaaatta ataatttaga tgaaagaatt ttagaagaaa aagtaggata taaaatttaa
    13501 aataaaaaaa taactatata aggagagata agatatgagt tacttaaaaa taaataatgt
    13561 gtttaaaagt tatgaccaaa aaagggtttt aaataatatt agtttggata ttgaagaagg
    13621 ggaatttcta tgtttactcg gacctagtgg atgtggtaaa actacattac ttagaataat
    13681 agcaggtctt gaagatgtaa atagtggaac tataattctc caggataaag atattacaaa
    13741 tttagagcca tcaaagagag gttttggtat agtatttcaa tcatatgcat tatttccgaa
    13801 tatgactgct tataacaata tagcttttcc actaaaagag agaaaagttt caaaagaaaa
    13861 aatagataat aaagttaagg aagtacttga aacagttgga ttgactaatg aagctcataa
    13921 atatcctaaa gcattatcag gtgggcaaca acagaggatt gcaatagcaa gagcattagc
    13981 tcttgaacca aagtttttac ttttagatga accaatgtcc gcattagatg caaaggttag
    14041 acacaagctt agaatggaca ttaaaagatt acaaaaagaa cttaatataa cgacaataat
    14101 ggtaacacat gaccaagaag aagctataac tatggcagat aaaatagcta ttctaaatgg
    14161 aggagatata atgcaaatag ggactccaga agaaatatac caaaatcctc aaaacctatt
    14221 tacagctcaa tttataggag atacaaactg ttttgataat ggagattcta tattaactgt
    14281 aagaccagaa tatgttcaaa tagagaaatc gacaaaagaa aactaccaag gtataatatc
    14341 aaatatagag tttagaggga atttattgag agttgaaata aaagataaat taaatgaaaa
    14401 tttcataata agtgatgttt ctataaaaga gtgggttaat ttaaatttag tagaaggtga
    14461 ccttgtaaaa ataagtatag acgaaaaata ctatttaaag tatccaaaag ttaaaggagc
    14521 ataagtgtat gaataataat gctataagtt ctaaaagtat aagtcttaaa tcaaaaatga
    14581 atttagatat aaaaaaatgt atgcttacag tattagttat gtctttagta atatttttat
    14641 tagcacctat aatatatcta tttgtacagg cttttttaga taaagatgga gtctttgtag
    14701 gattacaaaa ctttcaagaa tacttggctt ctgaatctct attaaattct ctaaaaaata
    14761 cagtatttgt atcagttata tcaactgtaa tatcactaac acttgcatta gtatatgcat
    14821 atgcattaac tagaacaaag attaaattta aaggtttttt taaatcatta gctatgtttc
    14881 ctatgtttgc tccaacaatg atgcatgcaa taggtctagt atatttattt ggaaatcaag
    14941 gtttggtaac taatttactt ggagtgcatg ttccaatata tggagcagtg ggaataatat
    15001 tggcacaaat tatatatgta tttcctcaat cctttcaaat actttatata tctctaagtt
    15061 caaccgatta tagactatat gaagctagta atactatggg gataagcaag ataagagaat
    15121 ttataagcat aacaatccca agtataaaat actctttaat aagttctgca ttcgtatcat
    15181 ttacattatg ttttacagat tttggagctc caaaggtgat aggaggagag tttaatgtat
    15241 tatcaataga tgtatataag caaattatag ggcaacagaa tatgccaatg ggagcaactg
    15301 tatcaatatt acttttaata cctgcaataa tatcgtttat agtaactaaa aaaattgaaa
    15361 aaaatcaaaa ctcatatata acttcaaagt caattccata taaggttaaa actaataagt
    15421 taagagatag tattttagga ggggttgtag gaattattgc atttggtata atagctgtaa
    15481 tgttaactgt aatcttatcc tcattaatat cagtttggcc atatgattta agacttacac
    15541 tggaacactt ttctatgaaa aacagtattg atggaattaa accatttata aattcaataa
    15601 aaatgtcact attgacagct attctaggga cgagctttgt tttcttattt gcatatttaa
    15661 atgaaaaaac agaaaaagct ccatatttaa aatcatttgg gtatttttta agtacattgc
    15721 ctatggcatt gccaggatta gttttaggta tagcttatgt attatttttt aataaattag
    15781 agtttaattt tatggataca atatacatta aaaatttgtt taatggatta tatggaacat
    15841 tacttataat ggttatatgt aatatagttc atttcttttc agtagcattt ataacagcta
    15901 ctactgctat taagaaagta gataaagaat tggacatggt agctaaatca atgggaataa
    15961 acagcgcatc aattcttaag aacgtaacta taccactttg tttgccagca gttttagaga
    16021 actttatgta cttttttcta aactctatgg tgacaatatc agcactagtt tttatatata
    16081 ctgcaagttc aaaagtagct gcaatatcaa ttgtacaact agatgataag gggagtactg
    16141 cacaggcagc agcactagct gtgttaatat tagtcacaaa tatattagtg aaagtaatat
    16201 atgaatttat aaagaatcgt ttagaaaaaa gaacaaactt taattaaata atttatctta
    16261 taaaaacaca aatttaaaaa gtctattctt atgatttatt atttaaagaa tagacttttt
    16321 aaatttatta ttgatattat ttattaattt tctttttgtt atcatacatt tgagaatctg
    16381 cacgacgata tgtatcaaat aaagaaacat ctactttttc atcaaaaata gcatatccaa
    16441 tagacattga taattttaat tcattagttg agttttcttc aactattttt aa
//
LOCUS       DAMJWP010000069        16391 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.071, whole genome shotgun sequence.
ACCESSION   DAMJWP010000069 DAMJWP010000000
VERSION     DAMJWP010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16391
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.071"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(286..708)
                     /locus_tag="PC700_001845"
     CDS             complement(286..708)
                     /locus_tag="PC700_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HDF2581156.1"
                     /translation="MNTFKTEVLLKYSAKDIFNIFTKSARLNFPNFNEKKAVGTFVQQ
                     KNNKRFKVEITNFEKNRVYEIKTSNKRESTITRYELVHIDLENTKLIFIESESKRNIF
                     GKINSALVRVLFGKRQPEEFNKFIKKLEMELENNSVNL"
     gene            1186..4089
                     /locus_tag="PC700_001846"
     CDS             1186..4089
                     /locus_tag="PC700_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2581157.1"
                     /translation="MELRDKLVNIINTENKKDPLTDVQIAKFLSTTRENITNLRKELN
                     ISNSRQRRYPYLKSAISAILQKNKNISISEITRELMTEGFNISRRVVEELLPKESLEM
                     DVVEESEEESNDPFETLIGNKGSLRNAVEQAKSAILYPPKGLPTLIIGESGVGKSLFS
                     RHMYEFAKQKKIVKESSNFVVFNCADYSDNPQLLLSLLFGYKKGAFTGAEYDTPGLVE
                     EADEGVLFLDEIHRLPPKGQEILFSILDRGKFRRLGETNAERKVSIMLIGATTENVET
                     NLLLTFRRRIPMLITLPPLHDRLLKEKIDLIYNIFQQECNRINAKIFVDKNVIEILVL
                     KKFSGNIGQLQNMIQVLCARAFMKFINSKDEDSESIVVVDINEVLKLKDSFKDAAFQE
                     IEYTEIRKYLKNAIFIPFNLEESSVKGSLLSDEYILPEDIYKTIEKKYYDLKSLDIED
                     IDIENILWTFILNRFADLKFNLDSNEEIFSMSDLNSFVSDSLVKLVKELMNDIVEKNM
                     NLEVNKNMFKYLAIHLEEAIKRIKLNQKIINVNLEKIKIDFSKEYEISKSFAIKIEES
                     ISIKIPDDEIGFITLYIKSALKNEVKKDKVGLIIISHGRIATETVNVVKELLGVKFPV
                     AIDMPLDEKPINIYNKAVELSKIIDQGKGILFLVDIGSLTNIGQIVNKRTNISTKTID
                     RVDLLMALEATRKVSMGEEELDEIFFSLLKDRMGYNYELEKHIDKSNAIVTLCLTGEG
                     TAKYISKTLEEKYDNTKCYQMSALDENLFCKIENLKETNNILAIVGTINPKIPGINFI
                     PYNKELFKNLDIYLSKSKQQDDRPVKSYERMLDEDLVIFEPDIYFKKDLLEYVCSILI
                     NKEYVEKDYLDSVLHREEMLPTYSKGAIGVPHGDSSTVNSTRFVFVKLKNPIDWGVGN
                     VNFIFMPVFQANDKEIVKNVLEILKDAEFMNNANKCFDKDAFRRIIFDKFKHL"
     gene            4122..4568
                     /locus_tag="PC700_001847"
     CDS             4122..4568
                     /locus_tag="PC700_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2581158.1"
                     /translation="MNVDLISLGLESNDYESVIEELGSIMCKKEYVKETYINAVLERE
                     RTLPTGLDIGEMCVAIPHTDSKHVNESNVAVGILKNPVKFNSMIDPKDRLDVELVFLL
                     AVKNPDSQVKLLKDLMSVFQNIKLLKNIKNASTKEEVAKLLDFIEI"
     gene            4691..4993
                     /locus_tag="PC700_001848"
     CDS             4691..4993
                     /locus_tag="PC700_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2581159.1"
                     /translation="MKQFNVLSVCGSGTVTSSMVAGKLKEVLGNKGISITTTEARPTE
                     ALNLAQSGRFDFITFTSPLQTGDYGIPTINAFACLTGIGEEAFFEEVLTVIEGIDK"
     gene            5174..6580
                     /locus_tag="PC700_001849"
     CDS             5174..6580
                     /locus_tag="PC700_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDF2581160.1"
                     /translation="MLEFMKSIIDTFGSAIIVPIIIFIIAKIFKVTTKKSFLSAVYAG
                     VALQGFTLILNSFTPIITPVINRMVESTGVNLPVFDVGWQATSLVAFSTSAGMIYLGL
                     GILLQTILFLIKWTDVFQPSDLWNNYSYMVWGAMVIGVTGNFPLGIACMVLLNLYSLL
                     ISELVAKRWSSYYRYPNCTIIAMHNVEASVFAVFSDPIYNKLGLNKIKLNPKELEKKL
                     GFLGEPITLGLFLGMFIGILGNMTRINTMEAWGEIMKVGISTSAVMAIFPKVASMFAQ
                     AFAPITEAARKIMQKAGNREWYIAVNDAVGYGEPATLISGLILIPIMLVISMVLPGNK
                     VLPVVDLLAIPYMVQGLVAIHNGNIPKVLVSGIIWFGLGLYVCTSTAPLFTDMATNIG
                     VAIPAGAMLITSFNILGKPLMGLVFFAFLSANPIYIGLSVVIYFVLWALFRKNKTSIL
                     DYLEKQALKNVEEEPVAV"
     gene            6924..7982
                     /locus_tag="PC700_001850"
     CDS             6924..7982
                     /locus_tag="PC700_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF2581161.1"
                     /translation="MGDKMRASVLYNVGDVRYEMVDIPEITDTQVLVNVKYVGICGSD
                     LPRSMVSGLSGNTKYPLILGHEFSGEVVKIGEKVKHINVGDRVAVAPLVPCGKCDYCN
                     EGNFGLCDDYNIIGTRVNGAFAEYVRVPEEHILKLPDTLDYETAAGIEPATIAYHGIS
                     KSNIRVGDSVVVLGCGPIGQFVIQWAKVFGASKIIAVDIFDEKLELSKLLGANYILNS
                     KEVNVIKEIKKITNGGADVVIETAGSRFTQEQSLFVAKKRGNIVFVGISHTELPLSAD
                     ATECILRGELTLKGSWNSYASPYPGRAWTATLDFMGKGDIIFKPMISHKIGLNEVGDF
                     LSKMSKREINFNKILVEI"
     gene            8057..8743
                     /gene="araD"
                     /locus_tag="PC700_001851"
     CDS             8057..8743
                     /gene="araD"
                     /locus_tag="PC700_001851"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDF2581162.1"
                     /translation="MLEILKEEVLKANLELPKKKLITYTWGNVSGIDREKGLVVIKPS
                     GVEYSNMTVEDMVVVDLEGNVVDGKLRPSSDTPTHLVLYKEFEELGGIVHTHSSWATI
                     WAQIGKSIPSCGTTHADYFYGSIPCTRKMTREEICNEYEKETGNVIIEEFMGRNPIHC
                     PGIIVNDHGPFTWGKDANEAVHNAVVLEEVAKMAYYTELMSPDNIMDKVLMNKHFSRK
                     HGKNAYYGQK"
     gene            8761..9408
                     /locus_tag="PC700_001852"
     CDS             8761..9408
                     /locus_tag="PC700_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HDF2581163.1"
                     /translation="MLDSLKGKVVEIAIKAEHYGLCKEKTGNFSIRDKDTGYILITPS
                     GIGREELNVEHICVLDLNGNVIEVEKGIKPSSEVLMHIEIYKIRENINAISHTHSLYA
                     TAFSVAKKPIIPIVYESVNYGGYVDIAPYERPGTLKLAKSVSKLLIDTDACLLERHGL
                     TTVGNDLDEALLKSRYVEEVAEIYYRSLVLNQFKELEVVDVDEFESWKYPEEIKF"
     gene            complement(9581..10948)
                     /locus_tag="PC700_001853"
     CDS             complement(9581..10948)
                     /locus_tag="PC700_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HDF2581164.1"
                     /translation="MNLIDILNKVDAFIWGPPLLVLLVGTGILLTVKLGVVQITKLPR
                     ALKLIFSAENKGSGDVSSFAALCTALAATVGTGNIVGVATAIKAGGPGALFWMWIAAF
                     FGMATKYSEGVLAIKYRTKDKNGQVSGGPMYYIVNGMGEKWRPLAIFFAISGILVALL
                     GIGTFTQVNSITDAINNSFGIDPRITGVVLAVFVALVVFGGLRSISNVATKIVPFMAV
                     IYVVICGIILISFWNKIPETFMLIIKSAFTPTAATGGFLGATMSLAIRNGIARGVFSN
                     ESGLGSAPIAAAAAKTEWPAEQGLISMTGTFIDTIIICTLTGFSLVISGVWCSDLNGA
                     VMTQAAFNGAIPTFGPILLTVSLTLFAFTTILGWSYYGERCFEFLFGVKGMNGYRTVF
                     VAMVLLGAFLKLEVVWIIADIVNGLMAIPNLIALLALSPIIVSETKKYFEHINSPENQ
                     IKKNA"
     regulatory      complement(11138..11226)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            11654..13774
                     /locus_tag="PC700_001854"
     CDS             11654..13774
                     /locus_tag="PC700_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDF2581165.1"
                     /translation="MGKILVLAEKPSVGRDIAKVLGSKNEKNGYIEGPKYVITWALGH
                     LVTLADPESYGERYKSWSLEDLPILPKHLKTVVIKKSGKQFNTVKSQMNRNDIDEIVI
                     ATDAGREGELVARWIIEKSQVKKPIKRLWISSSTDKAIKDGFAKLRSGKEYENLYYSA
                     IARAEADWIIGINATRALTTKYNAQLSCGRVQTPTLAMLLKREEEIRNFKPKEYYGLE
                     LIATKGNSDIKFIWNDKNNNSSTFSKEKIESILKKVKGVDIKISEVNKSYKKKYSPAL
                     YDLTELQRDANKMFGFSAKETLSIMQKLYEHHKVLTYPRTDSRYLTSDIVGTLKDRIK
                     AVNISDYSKICNKLLKSNIIANKSFVDNSKVTDHHAIIPTEQKVFMSDFSDKERKIYN
                     LVVKRFLAVLSPPFEYEQTTISATVEGEIFKAKGNKVINLGWKESYKDIEDEDEYESI
                     PNVLEKDVLKVKGLKITSGKTNPPPYLNEGTLLTAMEKNGLGTVATRADIIEKLFNSF
                     LMEKKNKEIYITSKGRQLLDLVPVDLKTPELTASWEKKLLDISKGKLSKNTFINDMKD
                     YSKDIVLEVKNSDNKFKHDNLTKNRCPECGKFMLEVNGKKGKMLICEDRECNTRKTIS
                     QVTNSRCPNCHKKLELRGEGEGRIFTCSCGYREKLSSFNKRKQEEKQKASKKDVNQYL
                     KNQNKNQETFNNPFAEALAKLKNK"
     gene            13878..15131
                     /locus_tag="PC700_001855"
     CDS             13878..15131
                     /locus_tag="PC700_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HDF2581166.1"
                     /translation="MDLSGIFKYYCKECENTWNNSSVELFEDIETYSKDSQKKREKEL
                     DKFINTISVHLERYPSDAVLRKMWVKKGEVFLQKTLEKENIFKLEKMDVEDRKKFLDI
                     TKQFIRDARKFDDDLPIGDIMQAMRNVWISNALQLLFGKEIYYSKANFAYSMLYPYTD
                     NYLDNTNIDKNDKILFNNWLEKRLLGEHIKSKDYHESKVSQMIDYIESVYPREKFTQV
                     YESLLLIFKSQVNSLKQHGKENHLCKEDLLSISIEKGGSSVLVDGYLISGFMTKEEIE
                     FCIGYGFLLQISDDLQDIKEDLKYNHKTIITEMSKEGTLDKVVNKLINFTIELIDSFK
                     INNKNKSVITMIKNDCLMLILFSVVYNAEFFSVGYIKEVEKFIPYTIDYSLEIEEKIK
                     EKFKNIDVLNNENEYKEMIDIICAE"
     gene            complement(15285..16346)
                     /gene="ytvI"
                     /locus_tag="PC700_001856"
     CDS             complement(15285..16346)
                     /gene="ytvI"
                     /locus_tag="PC700_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HDF2581167.1"
                     /translation="MDIQKQKNFIIKFTYVVLITSIVYIVLKFLLPLLMPFVISFIIA
                     SILRPIIRLITNNTNLNRTLISIVILLIFYGLCIFLLVSFGVKIFASVSDVFFRLPEI
                     YKSSIQPTLNTLFSKIDASTPNVNLSLILGWDNISQSMMSLVASISTNALNAIASIAS
                     KTPAFMLKLIITLIASFFFTFDYQKIVNFILKQFPEKSQFMIINIKNSSINALLKLLK
                     AYAILLSVTFLELLIGLTILKVENAFTISVIIALVDILPVLGTGSILTPWMIISLING
                     NINLAIGLLILYIIITVVRQILEPKVVGHQIGLYPLITLMCMFIGAQLFGIAGLFGFP
                     IAATIIKNLHDNGIITAFK"
BASE COUNT         6459 a         1869 c         2751 g         5312 t
ORIGIN      
        1 tatcttttct aaattagaag aggataatct tattagataa agattatcct ctttattgaa
       61 agtttaaggg atattagaga ttattaattt ttctctcttt ttttaactaa aagcaataat
      121 acataaaatt attattgcta atgatactct taatgtggta tatagtcata gtgtcacatg
      181 tttatcaata gttttttcat taattattgt aacgaatagt aggactcata aaatacttag
      241 aaatatgttt ttagatagat tttaaatata taatctaact tatatctata aattaacact
      301 attattctcc aattccattt ctagtttttt tataaattta ttaaattctt ctggttgcct
      361 ttttccaaac aatactctta caagagctga attgattttt ccaaatatat tcctcttgct
      421 ctcactttca ataaaaataa gttttgtatt ttctaagtca atatgtacta attcatatct
      481 agtaatagta ctttctcttt tattgcttgt ttttatctca tatactctat ttttttcgaa
      541 attagttatt tcaaccttaa atcttttatt atttttttgt tgtacaaatg ttcctactgc
      601 ttttttctca ttaaaattag gaaaatttag tcttgcagat tttgtaaata tattaaatat
      661 gtcttttgca gaatatttta ataaaacttc agttttaaat gtattcatat cgttttccct
      721 tctgtatata aatttaatat ttataagtta atttttaagt taaaatagta taatatgcca
      781 tattaaatca tgttaaaact atttatatag ctaaaacctt acctatataa gtatgtatac
      841 tatcatataa aaataaaatc ttaaaataga aagggttaaa aacacagttt attataattt
      901 ttattgttga gtagatacgt actattatat atgattgagc aagtttttca atcataaatt
      961 tataaagtat tttgcattaa aatttaaaca aagagttaaa ttatttaaga ttatgaataa
     1021 ctaatacatt tagtaaatta ctaaatatac aaaatgtaaa tacttatcaa aatgtataaa
     1081 tacttatgac atctagttac ttacagggtt taagtagaaa aaaatatcaa agtaagtatg
     1141 gcatgatagt tgcttatata tttatcaaga aaggagttat taagcatgga gttgagagat
     1201 aaacttgtaa acatcataaa tacagaaaat aaaaaagatc cattaacaga tgttcaaata
     1261 gcaaagtttt taagtacaac tagagagaac ataactaatt tgagaaaaga acttaatata
     1321 agtaattcaa ggcaaagaag atatccttat ttaaaatcag caatttcggc aatattacaa
     1381 aagaataaga atatttctat atcagaaatt acaagggaat tgatgacaga aggctttaat
     1441 atatcgagaa gagttgtgga ggaactttta cctaaagaaa gtttagaaat ggatgtagta
     1501 gaggaatctg aagaagaatc gaatgaccca tttgaaactc tgataggtaa taaagggagt
     1561 cttagaaatg ctgtggagca ggctaaatct gctatattgt atccaccaaa aggattacca
     1621 acgttgatta ttggggaaag tggagttggt aagagtttat tttctagaca tatgtatgag
     1681 tttgcaaagc aaaaaaagat agtaaaagaa agctccaatt ttgttgtatt taactgtgct
     1741 gattatagtg acaatccaca attattatta tctttgttat ttggatataa aaaaggtgca
     1801 tttacaggtg ctgaatatga tacaccaggt cttgtagaag aagctgatga aggagtttta
     1861 tttttggatg aaatacatag attgcctcca aaaggtcaag aaatactatt ttctatctta
     1921 gataggggaa agtttaggag attaggtgaa actaatgctg aaagaaaagt aagtattatg
     1981 ttgattgggg caactactga aaatgtagag actaatttat tattaacatt cagaagaagg
     2041 ataccaatgc taataacttt gccaccatta catgatagat tactaaaaga gaaaatagac
     2101 cttatataca atatctttca acaagagtgt aatagaataa atgcaaaaat atttgtagat
     2161 aaaaatgtga ttgaaatact tgtactaaaa aagtttagtg gaaatatagg acaattacaa
     2221 aatatgattc aggttctttg tgcaagagct tttatgaagt ttataaattc caaagatgaa
     2281 gatagtgaat cgatagtagt agtagatata aatgaagttt taaagcttaa agattctttt
     2341 aaagatgctg cctttcaaga gattgaatat acagagataa gaaaatattt gaaaaatgct
     2401 atatttatac catttaattt agaagaatca tcagtaaaag gaagtctttt aagtgatgaa
     2461 tatatattac cagaggacat atacaaaaca attgaaaaga aatattatga cctaaaatcc
     2521 ctagacattg aagatataga tatagagaat attttatgga cttttatttt aaatagattt
     2581 gctgacttaa agtttaatct cgatagtaat gaagaaattt tttcaatgag tgatttaaat
     2641 agttttgtaa gtgatagttt agttaaactg gtaaaagaac ttatgaatga tatagttgaa
     2701 aaaaatatga atttagaagt aaataaaaat atgttcaaat atctagcaat acacttagaa
     2761 gaagctataa aaagaattaa gctaaatcaa aagataataa atgtaaattt agaaaaaata
     2821 aagattgact tttcaaaaga atatgaaatt agtaaaagtt ttgcaataaa aattgaggaa
     2881 tcaattagta ttaagattcc agatgatgaa attggattta taacactcta cataaagtct
     2941 gctctaaaaa atgaagtgaa aaaagataaa gtagggttaa taataatttc tcatggacgt
     3001 atagctacag agactgtaaa tgtagttaaa gaacttttag gtgtaaagtt tcctgtggct
     3061 atagatatgc ctttagatga aaaaccaata aatatataca ataaggcagt agaactgtca
     3121 aaaataatag accaagggaa aggtatattg tttttggtgg atattggttc acttacaaac
     3181 ataggtcaaa tagtaaataa gagaaccaat ataagcacta aaactattga tagagtagac
     3241 ctcctaatgg cactagaagc aactagaaaa gtttctatgg gagaggaaga acttgatgaa
     3301 atattttttt ctttgctaaa agataggatg ggatataact atgaattaga aaaacacata
     3361 gataaatcaa atgcaattgt aacattatgt cttactggag aaggaactgc taagtatata
     3421 agtaaaacgt tggaagaaaa gtatgataac actaaatgtt atcaaatgag tgcattggat
     3481 gaaaatttat tttgtaaaat tgaaaatcta aaagagacaa acaatatatt agctattgtt
     3541 gggacaataa accctaagat accaggtata aattttatac cctataataa agaacttttc
     3601 aaaaatttgg atatctatct atcaaaatca aaacaacaag atgacagacc agttaaatcg
     3661 tatgaaagaa tgcttgatga ggatttagtg atttttgaac cagatatata ttttaaaaag
     3721 gatttattag aatatgtatg ttcaattctt ataaataaag aatatgtaga aaaagattac
     3781 ttagattctg ttttacatag agaagaaatg cttccaacat attctaaagg agctataggt
     3841 gtgcctcatg gagattcttc tactgtaaat agtacaaggt ttgtttttgt taaactaaag
     3901 aatcctattg attggggtgt aggtaatgta aatttcatat ttatgccagt ttttcaggct
     3961 aatgataaag agattgttaa aaatgttctt gagatactaa aagatgcaga atttatgaac
     4021 aatgccaata agtgttttga taaagatgct tttagaagga ttatatttga taaatttaaa
     4081 catctgtagc catagcaaaa aaattgagag gagttagagt aatgaatgta gatttaatat
     4141 cattgggttt agaatctaat gattatgaaa gtgttattga agaattagga agtataatgt
     4201 gtaaaaaaga atatgtaaaa gaaacataca taaatgctgt cttggaaaga gaaaggacat
     4261 taccaacagg tctagatatt ggagaaatgt gtgttgctat tccacataca gattcaaagc
     4321 atgtaaatga gtctaatgta gctgtaggga tattgaaaaa tcctgtaaag tttaattcaa
     4381 tgatagaccc aaaagataga cttgatgtag aacttgtatt tttattagca gtaaagaacc
     4441 cagattcaca agtaaaactg ttaaaagatt taatgtcagt ttttcaaaat ataaagttat
     4501 taaaaaatat aaaaaacgca tcaacaaaag aagaagtagc taaattattg gattttatag
     4561 aaatttaatg taggagaatt ttataaataa aaaattaaaa tttcaacaat caaagtaagt
     4621 attatttttt taataggttt aatcttatat aaaataaaaa tttagagtga aaaattagga
     4681 ggaaattatt atgaaacaat ttaatgtatt atcagtttgt ggttcaggga cagttacatc
     4741 atctatggta gcaggtaaat taaaagaagt actaggtaat aaggggattt ctattactac
     4801 aacagaagct agaccaacag aagcactaaa tttagcacaa tcaggaagat ttgactttat
     4861 aacatttacg agtccgcttc aaacaggcga ttatggaata ccaactataa atgcatttgc
     4921 ttgcctaact ggtatagggg aagaggcatt ctttgaagaa gttttgactg ttattgaagg
     4981 catagataag taagttttgg agacgtgaat ttatagtatt taaatcaatg atttagatac
     5041 tataataagc taatattaac taaatatttg tggaaaaaac attttatatg ttgtcataaa
     5101 ttaaaaaatt ataaaaatag tcacttaagt attaaattaa gcttataaat atttataagt
     5161 aggagggaaa aagatgttag aatttatgaa atctataatc gatacatttg gttctgcaat
     5221 tatagttcca attataatat tcataatagc aaagatattt aaagtaacta ctaagaaatc
     5281 gtttctttct gctgtgtatg caggagttgc tttacaaggt tttacactga ttttaaattc
     5341 atttacacca ataataacac cagttatcaa taggatggtt gagagtacag gagtaaacct
     5401 accagttttt gatgttggtt ggcaagcaac atcattggtt gcattttcaa caagtgcagg
     5461 tatgatatat ttaggtcttg gtatattatt acaaacaatt ttatttttga taaaatggac
     5521 agacgtgttt caaccatcag atttatggaa taactattct tatatggtat ggggagcaat
     5581 ggttatagga gtaacaggaa attttccttt aggtatagca tgtatggttt tattaaactt
     5641 atatagttta ttaatatcag agttagttgc aaagagatgg tctagttact atagatatcc
     5701 taattgtaca ataatagcta tgcataatgt tgaggcttct gtgtttgcag tattttcaga
     5761 ccctatatac aataaattgg gattaaataa aataaaatta aatccaaaag aattagagaa
     5821 gaaattaggg tttttaggtg aaccaattac tttaggtctt tttttaggta tgtttatagg
     5881 tatattaggt aatatgacga gaataaatac tatggaagct tggggagaaa taatgaaagt
     5941 aggtatatct acaagtgcag tgatggcgat tttccctaaa gtagcaagta tgtttgctca
     6001 agcatttgca cctataacag aagcagctag aaagattatg caaaaagctg gaaaccgtga
     6061 gtggtacata gcagtaaatg atgctgttgg atatggtgaa cctgcaactt taatatcagg
     6121 tcttatatta attccaataa tgcttgtaat atctatggta cttcctggta ataaggtatt
     6181 accagttgtg gatttgttgg caattccata tatggttcaa ggtttagttg ccatacataa
     6241 tggtaatata ccaaaagtat tagtttcagg tataatttgg tttggattag gattatatgt
     6301 ttgtacatct acagcaccat tatttacaga tatggctaca aatataggtg tagctatacc
     6361 tgcaggagca atgttaataa caagctttaa tatactagga aaacctttga tgggattagt
     6421 tttctttgca tttctaagtg caaatccaat ttatataggt ctttcagttg taatttactt
     6481 tgttctatgg gcattgttta gaaagaacaa aacaagtatt cttgattatt tagaaaaaca
     6541 agcacttaaa aatgtagaag aagagcctgt agcagtttaa gtttataaaa atgaaataag
     6601 aataattaaa ggagtttata attgatttta tataagtatc agataatacc tgagaaatat
     6661 ttaaatgaaa tatagacttc tttttatttg ataatatttg taattataat gcattgtatt
     6721 tttatagttg agttttctgt ataacctgct atttaaaaca tactctttta tatttataca
     6781 gtaatgtggt ttaattttat aaaatgtaat ctctactggt aaaagacttt gtatataagg
     6841 catttgcctg aataaaccat ttatcttttg tatggcatag atattgctat atagaagtat
     6901 atagcatgaa aggagatatt tatatgggag ataaaatgag agcatcagta ttgtataatg
     6961 taggtgatgt caggtatgaa atggtagata taccagaaat aacagatacg caagtgcttg
     7021 taaatgtaaa atatgttggg atatgtggtt cagatttgcc taggtctatg gtaagtggtt
     7081 tgagtggaaa tacaaaatat ccattgattt taggacatga attttctggt gaagttgtta
     7141 aaattggaga aaaagtaaag catattaatg taggagatag agtagctgtt gcaccattag
     7201 taccttgtgg aaaatgtgat tattgtaatg aaggtaattt tggcttgtgt gatgattaca
     7261 atataattgg aacaagagta aatggagcgt ttgcagaata tgtaagagtt ccagaagaac
     7321 atattttaaa actaccagat acattggatt atgaaactgc tgccggaatt gaaccagcaa
     7381 ctatagctta tcatgggatt tcaaagtcta atattagagt aggagatagt gtagttgtgt
     7441 taggatgtgg tccaataggt cagtttgtta tacaatgggc aaaggtgttt ggtgcatcta
     7501 agattatagc agttgatatt tttgatgaaa aattagaact ttctaaactt ctaggagcaa
     7561 actatatact taactcaaaa gaagtaaatg taatcaaaga aataaaaaaa ataacaaatg
     7621 gtggtgctga tgttgtaata gagactgctg gtagccgatt tacacaagaa caatctctat
     7681 ttgtagcaaa aaagagagga aatatagtgt ttgtaggtat atcacacaca gaattaccac
     7741 ttagtgcaga cgctactgag tgtatattac gtggagaact taccttgaaa ggttcttgga
     7801 attcatatgc aagcccttat cctggaagag catggactgc aacattggat tttatgggaa
     7861 aaggtgacat tatttttaaa cccatgattt cacataaaat aggtcttaat gaagtgggtg
     7921 actttttaag taaaatgagc aagagagaaa ttaatttcaa taaaatccta gtagaaatat
     7981 aaaaatttaa taaaaaattc taaaaacaat taaattttat aactatgaca ttaaataaaa
     8041 tataggagga agaaaagtgc tagaaatttt aaaagaagaa gtattaaaag caaacttaga
     8101 attacctaag aaaaagctaa ttacttatac ttggggaaat gtaagtggga tagatagaga
     8161 aaaaggatta gtggtgataa aaccaagtgg tgtagagtat agtaatatga ctgtagaaga
     8221 tatggttgta gttgatttag agggaaatgt agttgatggc aaactaagac cctcctcaga
     8281 tactccgact catctagttt tatataaaga gtttgaagaa ttggggggaa tagtacatac
     8341 acattcaagt tgggcaacaa tttgggctca aataggaaaa tctataccat cttgtggtac
     8401 tactcatgca gattattttt atggaagcat accatgtacc agaaaaatga ctagagaaga
     8461 aatttgcaat gaatacgaaa aggaaactgg aaatgtaatc attgaagaat ttatgggaag
     8521 aaatccaatt cattgtccag gaataattgt aaatgaccac ggacctttta catggggaaa
     8581 agatgcaaat gaggctgtac acaatgcagt agttttagaa gaagtcgcaa agatggctta
     8641 ttatacagag ttaatgagtc ctgataatat tatggataag gttcttatga ataagcattt
     8701 ttcaagaaaa catggaaaaa atgcatacta tggtcaaaaa tgattatctg gaggtgaatt
     8761 atgctagact ctttaaaagg taaagttgtg gaaatagcaa ttaaagcaga gcattatggt
     8821 ttatgtaaag aaaagactgg taattttagt attagagata aggatactgg atatatactt
     8881 ataaccccat caggtatagg aagagaagaa ttaaacgttg aacatatttg tgtattggat
     8941 ttgaatggaa atgtaataga agttgagaaa ggtattaagc caagtagtga ggtattgatg
     9001 catattgaaa tatataaaat aagggaaaat ataaatgcta tatcacatac tcattcttta
     9061 tatgcaacag cattttcagt agcaaagaaa ccaattatac caattgtata tgaatcagtt
     9121 aattatggcg gttatgtaga tattgctcca tacgaaagac caggaacttt aaaattagct
     9181 aagagcgttt caaaattact gatagataca gatgcatgct tattagaaag acatgggttg
     9241 acaactgtag gtaatgattt agacgaagca ttattaaaat caagatatgt tgaagaggta
     9301 gcagaaatat actataggtc tttagtatta aatcaattta aagaacttga agtagtagat
     9361 gttgatgagt ttgaatcttg gaaataccca gaagaaataa agttttaatc taaataattt
     9421 tcattgaagt ggttaattaa aatatatgta ttatttatat caattactat gttttattat
     9481 attaatcacc gtattttgta aatatattta ttacattttt tcaaaaaaat atagaatccg
     9541 aaagccatat aaaattgact tgggattcta tactttttat ttatgcattt ttctttatct
     9601 gattttctgg agaatttata tgttcaaaat attttttagt ttcagatact attataggtg
     9661 ataatgcaag taaagctata aggttaggta ttgccataag cccatttaca atatcagcaa
     9721 ttatccaaac aacttctaac tttaagaaag cacctaataa caccatagcc acaaatacag
     9781 ttctataccc attcatgcct tttacaccaa acagaaattc aaaacatctt tctccataat
     9841 aactccatcc caaaattgta gtaaatgcaa ataatgttaa actaactgta agtaatattg
     9901 gtccaaatgt aggaattgct ccgttaaaag ctgcttgtgt cataacagct ccatttaggt
     9961 ctgaacacca cactcctgaa ataacaagag agaatccagt aagagtacag attataatag
    10021 tatctataaa agtacctgtc atagatataa gaccttgttc cgctggccat tcagttttag
    10081 cagcagctgc agctattgga gcactaccta gaccagactc atttgaaaat acaccacgag
    10141 caataccatt tctaattgcc agtgacatag ttgctcctaa aaaaccacca gtagcagcag
    10201 taggtgtaaa tgcactttta attattaaca taaaagtttc agggatttta ttccaaaaag
    10261 atattaaaat gattccacag attacaacat atataactgc cataaaaggt actattttag
    10321 tagctacatt agatatactt ctaagtcctc caaatacaac caaagctaca aatactgcta
    10381 atacaacccc tgtaattcta gggtctatcc cgaaactatt atttattgca tctgttattg
    10441 aatttacttg agtaaaagta ccgattccta aaagtgcaac tagtatacca cttatagcga
    10501 aaaatattgc aagaggtctc catttttctc ccataccatt tactatgtaa tacattggcc
    10561 caccagacac ttgaccattt ttatctttag ttctgtattt tattgcaaga acaccttctg
    10621 aatacttagt agccattcca aagaatgctg ctatccacat ccaaaaaagt gcaccaggtc
    10681 caccagcttt gatagctgta gcaacaccaa caatattacc agtaccaact gtagcagcta
    10741 gagcagtaca taaggctgca aaacttgaaa catcaccact acctttgttt tcagcagaga
    10801 atattaactt aagagctctt ggcagtttag ttatttgaac aactccaagc ttaacagtaa
    10861 gtagtatacc agtaccaact aatagtacca gtaggggtgg tccccaaata aaagcatcaa
    10921 ctttgtttaa aatatcaatt agattcataa aaacactccc tcattttttt tgctttacat
    10981 aatcaataga atttttaatg aagtaattga caggaaataa gtataattta ttaataattt
    11041 ctagtgtaat aaaaaaagct aaatctaaaa aagattcagc tctggatgta cactaattag
    11101 caaccaaaat aaaagtatta gtaaatactg tttttggtag ataatccctg tccttttgcc
    11161 tgagagattg agcataaaag ccttgcccct tcggcgccca taatgggtct ctccagagtt
    11221 ccgtaaattt tacagtccta cttttaaaag tacctgagag tgttactcct tcggtgtgct
    11281 atatagcaac tcttctataa aatcttaacc ggatattgat ttgtcatagc ttattgtatt
    11341 aataatatta atataagttt ttatgttttg tcaactaaaa aattaaaaaa caaagaaaaa
    11401 atataaaaat gtaagcctat ttacgaatta taaacttaga aagatgagta atatacgtaa
    11461 aaatgaattt tgtaatacac atacaaatga agtgtaaaat aagttttaaa taaaaaaata
    11521 ataaaatctt aaataattaa ttagatatga ttaaattatg gtaatattat agttacgata
    11581 ttaagaaaag ttctatttag ttagatttta agcgtaatat gagattatgt atagagaaag
    11641 gatgtatttt tagatgggaa aaatattagt gctagcagaa aaaccttctg ttggaagaga
    11701 tatagcaaaa gttttagggt caaaaaatga aaaaaatggt tatatagagg ggccaaagta
    11761 tgtgataact tgggcactag gtcatttggt tacacttgct gaccccgaaa gttatggaga
    11821 aagatataaa tcatggagct tagaagacct gccaatactt cccaagcacc ttaagactgt
    11881 tgtaataaaa aagagtggaa agcagtttaa tacagttaaa tcgcaaatga atagaaatga
    11941 tatagatgaa atagttatag caacagatgc aggtcgtgaa ggagaacttg tagcaaggtg
    12001 gataatagaa aaatcacagg taaaaaaacc tataaaacgt ctttggattt cttcaagtac
    12061 agacaaagca ataaaagacg gatttgcaaa attaagaagt ggaaaagaat atgaaaattt
    12121 gtattactca gcaatagcac gagcagaagc tgattggatt attggaataa atgcaaccag
    12181 agctttgaca acaaagtaca atgcacagct ttcttgtgga agagtacaaa ctcctactct
    12241 tgcaatgttg cttaaaagag aagaagaaat aagaaatttc aaacctaaag agtattatgg
    12301 attagaatta attgctacaa agggaaattc agatataaaa tttatttgga atgataaaaa
    12361 caataattct tcaactttta gtaaagaaaa gatagaatcc atattaaaaa aagtaaaagg
    12421 tgtagacata aaaatatctg aagtaaacaa gtcatataag aaaaaatatt cacctgcact
    12481 ctatgattta acagaattac aaagagatgc aaataaaatg tttggatttt cagcaaaaga
    12541 aactttatct ataatgcaaa aactgtatga acatcataaa gtacttactt atcctagaac
    12601 tgattctaga tatttaacat cagatattgt tggaacatta aaagatagaa taaaagctgt
    12661 aaatattagt gattattcaa agatttgtaa taaattatta aaaagcaata ttatagctaa
    12721 taaatcgttt gttgacaatt caaaggtaac agaccaccat gcaataatac caacagaaca
    12781 gaaggtattc atgagtgatt ttagtgacaa agagcgaaaa atatataatc ttgtagtaaa
    12841 aagattttta gcagtactat ctcctccttt tgaatatgag cagacaacta taagtgcaac
    12901 tgtagaagga gaaatattta aggctaaggg aaacaaagta ataaatttag gttggaaaga
    12961 aagttataaa gatattgaag atgaagatga gtatgaaagt ataccaaatg ttttagaaaa
    13021 agatgtactt aaggtcaagg gattaaagat aacttcagga aaaacaaatc caccaccata
    13081 cttaaatgag ggaacattat tgactgctat ggaaaagaat ggtctaggga cagttgctac
    13141 aagagcagat ataatagaaa aattattcaa ttcattttta atggaaaaga aaaataaaga
    13201 gatatatata acatcaaaag gaagacaatt attagattta gttccagtag acttaaagac
    13261 accagaactt acagcttcat gggaaaagaa attattggat atttcaaagg gaaaacttag
    13321 taaaaataca ttcataaatg acatgaaaga ttattcaaaa gatattgtac tagaagtaaa
    13381 aaatagtgat aataaattta aacatgataa cttaactaaa aatagatgtc cagaatgtgg
    13441 taaatttatg ttagaggtaa atggtaaaaa aggtaagatg ttgatttgtg aagatagaga
    13501 gtgtaatact agaaaaacta tatcacaagt aaccaattca agatgtccta actgtcataa
    13561 aaaattagaa cttcgtggag aaggtgaggg gagaatcttc acttgtagtt gcggatacag
    13621 agaaaagttg tcttctttca acaagagaaa gcaagaagaa aaacagaagg catcaaaaaa
    13681 agatgttaat caatatttaa aaaatcaaaa taaaaatcaa gagacattta ataatccatt
    13741 tgcagaagca ttagctaagc taaaaaacaa ataattttaa agttattatt atacacaatt
    13801 ataactttaa ctgtatatta gttatatgta ctgatataat atagtgtata gtttaggcat
    13861 gaaagtaggg ctgttaaatg gatttaagtg gcatatttaa atactattgt aaagagtgtg
    13921 aaaatacatg gaataattcg agtgttgaat tatttgagga tatagaaacg tatagtaaag
    13981 attcacaaaa aaagagggaa aaagaattag ataaatttat aaatacaata tcagttcatt
    14041 tagagaggta tccaagtgat gctgtattga gaaaaatgtg ggtaaaaaag ggcgaggttt
    14101 tcttacaaaa gacattggaa aaagaaaata tttttaagtt agaaaaaatg gatgtagagg
    14161 atagaaaaaa atttttagat ataacaaaac agtttattag agatgctaga aaatttgatg
    14221 atgatttacc tataggtgat attatgcaag ctatgaggaa tgtatggatt tcaaatgcat
    14281 tacaattatt atttggtaaa gaaatatatt attcaaaagc taactttgca tatagtatgt
    14341 tatatccata tactgataat tatttagaca atacaaatat agataaaaat gataagattt
    14401 tatttaataa ctggttagaa aaaaggctcc tgggagaaca tattaaatct aaggattatc
    14461 atgaaagtaa agtatctcaa atgatagatt atattgaaag tgtataccct agagaaaagt
    14521 ttacacaagt ttatgaatcg ttattattaa tatttaaaag tcaagtaaat agtttaaaac
    14581 aacatggtaa ggaaaatcat ttgtgtaaag aagatttatt atccatttct attgaaaaag
    14641 gaggttcatc cgttttagta gatggatatt taataagtgg atttatgaca aaggaagaaa
    14701 tagagttttg tataggatat ggatttttat tacaaatatc tgatgattta caagatataa
    14761 aagaagattt aaaatacaac cataaaacta ttattacaga gatgtcaaaa gagggtactt
    14821 tagataaagt tgtaaataaa ctaataaatt ttactattga gttaatagat agttttaaaa
    14881 ttaataataa aaataaatct gtaataacta tgataaagaa tgattgctta atgttaattt
    14941 tattttctgt agtttataat gctgaatttt tttctgtagg atatataaaa gaagtagaga
    15001 aatttattcc atatacaata gattattcat tagagattga agaaaaaata aaagaaaaat
    15061 ttaaaaatat agatgtttta aataatgaaa atgaatataa agaaatgatt gatattattt
    15121 gtgcagagta gaaaaaaagg gagtgtctta gaatagactt tttagtttat gaggcactct
    15181 ttttgtataa aatcaaatgt attttcatca ttttaacaat taaaaagagc ttatccctgt
    15241 ttgagaaagc cctttttaat ttaaataaaa atgtcaaggt taaactattt aaatgcagta
    15301 ataatcccat tatcatgtaa gttttttatg attgtagctg cgatagggaa tccaaataat
    15361 ccagctattc caaatagttg agctccaata aacatacaca ttaatgttat taaaggatat
    15421 aggccaattt ggtgtcctac tacctttggt tctaatattt gtctaacaac ggttataatt
    15481 atgtatagta ttaaaagtcc tattgctaaa ttaatatttc cattaatgag tgagataatc
    15541 atccaaggtg tcaaaatact tcctgtaccc aagactggca aaatatcaac aagtgctatg
    15601 atgacagata tagtaaatgc attttcaacc tttaggatag taagtccaat aagtagttct
    15661 aagaatgtca ctgatagtag tattgcatag gcttttagta gttttaaaag agcattaata
    15721 ctgctatttt ttatatttat tatcataaat tgactttttt ctggaaattg ttttaagata
    15781 aagtttacta ttttttgata gtcaaaagta aagaaaaaag aagcaattaa agtaataata
    15841 agttttaaca taaatgcagg tgttttactt gcaatactag caatagcatt gagtgcattt
    15901 gttgaaatag aagctactaa agacatcata gattgactta tattatccca accaagtatt
    15961 aatgataggt taacattagg tgtactagca tctatctttg aaaataaagt atttagcgtt
    16021 ggttgtatac ttgatttata tatttcagga agtctaaaaa atacatcact tacacttgca
    16081 aatatcttca caccaaagct aactaataaa aatatacaca acccataaaa aattaaaagt
    16141 attactatag aaataagtgt acgatttaaa tttgtattat ttgtaataag tcttataata
    16201 ggtcttaaga tggatgcaat gataaaactg attacaaaag gcattaataa tggcaataag
    16261 aattttaata caatatatac aatacttgtt attaaaacta catatgtaaa tttaattata
    16321 aaattctttt gcttttgaat atccataata taaaacctcc aatgtatttg atattattct
    16381 aaatatggtc t
//
LOCUS       DAMJWP010000070        15977 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.074, whole genome shotgun sequence.
ACCESSION   DAMJWP010000070 DAMJWP010000000
VERSION     DAMJWP010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15977
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.074"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(7..2499)
                     /locus_tag="PC700_001857"
     CDS             complement(7..2499)
                     /locus_tag="PC700_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2581168.1"
                     /translation="MNHNTSIKNKDSNPKIFLVLTLYLLGIFMGAIDTGIVSPARTII
                     QNSLGVNEKTGIWMITIYTLTYASVIPISGKLADKIGRKYVYLVSIFLFGSGSLICGL
                     SSLFSNFYILLIGRVIQAIGGGGIMPIATAEFGTTFPENKRGMALGLVGATYGIANIL
                     GSSIGSTILSIFGTQNWKWLFFVNLPICLIILIGGVFCIKNNKSESKEKIDKLGTLML
                     ICIIVSLLYGLMNIDFFNFKNSIQDISVYPYLLTFIVLIPIFIFIENRAKDPILSFEY
                     FLNPRILIVLILSLIVGIGMMGMVFVPQYAENALKINAGSGGYFVAILGLFAGIAAPL
                     SGRLIDKFGAKKILLLGFSVSMIGSLYLVLIALKTNTVFSVCVSLIFMGLGMGFTMGT
                     PLNYMMLSNTRLEESNSALATLSLIRSIGTSISPAIMIGFIAHAGLSVQDNIMDLVGK
                     PSTPKIVQLEELENMIDDLKSDPEMAKNLKNVSIPNMNDTSNINMDMTGGKLPKSLLN
                     KVQSADTTNITNITKEISTTMFDEKVPSIITKIQKNVQKGIDGTQSGINGIEDGENKL
                     NSGIKGVQTGIDNMVKARAGINQGIDGVKKGIAGVDKGIKGMEQGLEKQDEAIKELTT
                     AYNKIPTTEKTPTDNENNSNEQNQSNTSNSESDDTKNDSTENKNNNSSENKPNVNEQK
                     ESLNAQIQQLKKTRDDLNTKLQKNKTQKNELSKKLRSMESQKKELQSKLNNSINQKKD
                     MEKSLDTMQQQKKSLQAVLAKTQEVKTEIPKAFDKSKLDYINSIENNRTKIENTLQST
                     LNSGFKQMYITVFCVNLLAFIILLFYKENKTR"
     gene            complement(2513..3055)
                     /locus_tag="PC700_001858"
     CDS             complement(2513..3055)
                     /locus_tag="PC700_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2581169.1"
                     /translation="MPKILENVKEDILKVSRDMILEEDYSNISIRKIAQRCGISAGTV
                     YNYFNSKQEIIEYITKSEWDLLIRRIEYSNKNTEDYIKKLNIIFTEIRNFINKVHNIQ
                     YNDFLNTFETERFFEMKKHKDDFHEQLSNKVYEALEKESFFNNDKLVCNIIIKMFFSY
                     STYLHIEFEDLKPYIEKLIH"
     gene            3298..4812
                     /gene="glpK"
                     /locus_tag="PC700_001859"
     CDS             3298..4812
                     /gene="glpK"
                     /locus_tag="PC700_001859"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDF2581170.1"
                     /translation="MEKKYVMALDQGTTSSRAILFNKKGEIVKIAQKEFNQIYPKAGW
                     VEHDPMEIWGSQSGVMREVIETAGIRPEEIASIGITNQRETTVVWSRYTGKPIYNAIV
                     WQCRRTSEICDELKNKGLEESIKEKTGLLIDAYFSATKVKWILDNVEGAREKAEQGEL
                     LFGTIDTWLIWNLTRGKVHVTDYSNASRTMMYNINDLEWDEDILRELDIPISMLPLVK
                     PSSYVYGHTDERMLSGAKIPIAGCAGDQQAALFGQNCVEEGTAKNTYGTGCFLLMNTG
                     SNIVKSKHGLLTTIAWGVDGKVTYALEGSIFIGGASVQWLRDELKIIESAKDSEMYAN
                     RVEDTNGVYVVPAFTGLGAPYWDMYARGSILGLTRGAKKEHIVRATLESIAYQTKDVL
                     EAMQNDSKLKLKSLKVDGGASNNNFLMQFQSDILNVDIDRPKIVETTALGAAYLAGLS
                     VGFYIGRNEITSKWSVEKEFNPNMSEEKRCKLYKGWKKAVSRALSWEKEDELDI"
     gene            complement(4926..5555)
                     /locus_tag="PC700_001860"
     CDS             complement(4926..5555)
                     /locus_tag="PC700_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2581171.1"
                     /translation="MDKNYTMPIYQKIALDIANKIYTGEIQEDSVLFGRSVLAGKYNV
                     SPETIRRAVKILEDIGVVKSIKGKGVIVLSPDKASSFIKKYRDITNISSYKSTLYNLI
                     DTKSNLENEILDTINKILDYSNRLEIINPLVPVQFTINSNCKYIGQTAAQTKFWQNTG
                     ATIVAIKRGEELIISPGPYIEFLEGDILLVVGDQHIYNSIPMFLYENEK"
     gene            6144..6935
                     /locus_tag="PC700_001861"
     CDS             6144..6935
                     /locus_tag="PC700_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HDF2581172.1"
                     /translation="MYKVDLNSDLGESFGTYKIGLDEEVLKYISSANIACGFHAGDPS
                     HMEKTVQLAKKNGVKIGAHPGFLDLIGFGRREMKITKQEAKDYTKYQLGALMAFASSN
                     GCNIQHVKPHGALYNMAAKDKELAMGICEAIYEVDKDIILLGLYNSEMINSAKEIGLR
                     FANEVFADRAYDNNGFLVPRSVEGAVIHDTKHAIDRVVRMVKEGTVETLTGEVIHIKA
                     DSICVHGDNPKAIEFVKEIRKRFELEGIEVCPLENIEVCSSENIV"
     gene            6964..8184
                     /locus_tag="PC700_001862"
     CDS             6964..8184
                     /locus_tag="PC700_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HDF2581173.1"
                     /translation="MSTKNITDKTKNKKDVGALIGAAFIMATSAIGPGFLTQTAQFTQ
                     DFGPNFSFVILITTILFIGAQVNVWRVIGVSGLRGQDIANKIIPGLGYLVAFLVALGG
                     LAFNIGNVGGAALGMNVMFNMNMTLGTVLSGLIAIFVFMSKNSNSLVDKITKFLALGM
                     IIIVGYVAISNHPPVGEAVSRMVKPENPKGLIFPIITLLGGSVGGYITFAGGHRLIDG
                     GITGEENIKEITKSSLLGILVDTMMRVLLFLAILAVVSKGLQLDPENPAASAFKFSAG
                     AIGYKFFGLVLWSAAITSVIGAAYTSVSFLKTLNPFIDKYEKYFIIAFIAISTLIMAF
                     IGKPATLLILAGALNGLILPITLGIMLIASKRKDIVGDYKHPTWLLIFGLIVVLISAY
                     TGITSLSSLGALFA"
     gene            8204..8899
                     /gene="pxpB"
                     /locus_tag="PC700_001863"
     CDS             8204..8899
                     /gene="pxpB"
                     /locus_tag="PC700_001863"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HDF2581174.1"
                     /translation="METRYLISGDKAVVAEFGNEISEDINKKVISFMRAIEISNLKGI
                     VTEMVPTYRSLMISYNPLEIDFDSLIENLKKIEDNLESIVLPKPKIHEIPVCYDEVFG
                     IDIKNVASYNNLTVDEVIKIHTSREYLIYMLGFTPGFPYLGGMDERIATPRLEVPRTK
                     IYGGSVGIAGSQTGVYPIDSPGGWQIIGRTPLKLYDENREEQILLRAGDFIKFVPITL
                     DEFIEIEKNNLSI"
     gene            8929..9915
                     /locus_tag="PC700_001864"
     CDS             8929..9915
                     /locus_tag="PC700_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase"
                     /protein_id="HDF2581175.1"
                     /translation="MGFKVELGGFQTLIQDRGRVGYGQYGVSGCGAMDEYAHRVGNIL
                     VGNSEDEASLEVLMLGPTITFDEYTQIAVTGGDLGAKINGKEIQNWRSYQINPGDVLS
                     FRGVKSGARAYVSIAGGIDVPLAMGSKSTYTRSKIGGFEGRALKKGDYINTFIQEKDF
                     TINKKLNSKYIPTYSSEIVLRIVKGPQFDAFSNGEVEKFLSNKYKVTNEIDRMGCRLD
                     GESIKHLNGADIISDGISYGAIQVPGHGKPIIMLSDRQTSGGYTKIGNVISVDLYKLA
                     QAKPNDVVKFELVDIYEAHRLLREQEDKIQDIYKSMKNIRVVKAKVLNDIAV"
     gene            complement(10134..10700)
                     /locus_tag="PC700_001865"
     CDS             complement(10134..10700)
                     /locus_tag="PC700_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2581176.1"
                     /translation="MLGEKMRNIRKSKKKTLSDVSKLTDLSISYISQIERDAIEPSLS
                     SLRKIAEVLDTPLYMFMDDNNTDDLVIRKEDRVMMKFPKSEMFYEIVSPMPTTEFTPS
                     ILFVEFELKPESEDAKDYIYHASEEIMVVTSGVVDICMGEKVVRLNPGDSTFIKANVP
                     HKIINPSKDTIARGYGVISPPIWPIKSK"
     gene            11052..12119
                     /locus_tag="PC700_001866"
     CDS             11052..12119
                     /locus_tag="PC700_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HDF2581177.1"
                     /translation="MKITDIKFEKLRIKLKKPVVVSFGVIEYGESVILKIETDEGYCG
                     FGEAAPLAAVTGEVLDNVLSVLLMFKKELIGKDPLDIETIHTIMDGIIIGNTSAKAAI
                     DIALYDIKGKIMNAPLYKVLGGFDSKVQTDITISIDKPEKMAVEALERVKEGFRILKL
                     KAGINPEDDIEAVKLIREAVGESIRIRIDANQGWNVNSSINTIKKLEEFNVDAIEQAL
                     PHWDLDGTAYIRNKSNTKIMIDESLHSPIDAIKAIKKNAVDTFNIKLMKSSGIYPAIK
                     INNIAEASGVNCMLGCMLETRIGITAAANLIASKKNITEADLDSFMFCEESKDISGGF
                     VMDGDIMNLVNKPGLGIEVNL"
     gene            12138..13298
                     /locus_tag="PC700_001867"
     CDS             12138..13298
                     /locus_tag="PC700_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HDF2581178.1"
                     /translation="MNTLFSNPGSILAVMTSMIALGFYLQRYKAIKSLGPALTIIIMG
                     IILSNLKVVPVSTELYGTISTYAIPVSMTIMLMSVDLKEMTKLSREPLIAIFVAVLTV
                     SIMAFLFGLVFAEKISEGWKVAGMFVGTYTGGSANLTAIGTGLNVSRQTLAAANAADY
                     VIGVPTLIFMFALPAILKNSKKFKKLWPYHVEETELEDCQNEEFMESKEWSIKDIAWM
                     LAIGFVVTEVSTILAGYFNSSFSSAARILLVTTISIIIAQLKPVKKLKGNLDLGLFVA
                     LFFLCTIGFSVDIKEFLGSTFTITLYCFSIIFASFVFHLGITRLLKIKYQYVILSIVG
                     AIADGPTSALVAASAKWNSLVSVAVVMGVIGGVLGNYAGISVAYAIKMSLGL"
     gene            13311..15407
                     /locus_tag="PC700_001868"
     CDS             13311..15407
                     /locus_tag="PC700_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HDF2581179.1"
                     /translation="MIKFKLYISGLYSGNVIFDGDLLIEKLNPFTNKIESLKPISKEE
                     NTYYLNLTKIDLKSLFNNFDVYTKSLVNTDKDTVINNLGETHSKLNEYIWVQRNKKFP
                     LDIIIVDNKIVGFICLSRETCTILIMDGYEEYTVLKEWEKTHKNEEIYSIRFGGNYMI
                     DMKDGIKLSTDVYLPDFVDSTKKAPTILMRTPYGKENDKEIYYKYVQRGYAVVIQDVR
                     GRNESEGKWEPMIHEREDGDSTINWIVSQEWSSGIVGMLGASYLGYVQWAAASSGNKH
                     LKALVSIVTSGSPFIDIPRKGGAFVSGMLAWAFMVSRNKVDRSKMVRDDWDDVLNIRP
                     IENIPVEALGYRIEFLEEWLKRVEKDEYWDLMDWHLQKDKINVPALVVSGWYDDNSMG
                     TTEALDVIKDYEKGKRKAILGPWMHNSNTLRDINGISLGNSSLRYDLDYNYLLWFDKY
                     LKGIENNIDTTAPVEYYSVGFNKWKTEENWPIINKIDKSMYLISDGNANTSLGNGRLV
                     FDNDLEEKYDSYIYNPKDPSVQLIDMSENEVGVPNNYKDLEKRSDMLCYTSDAFSEEF
                     TVTGDIKLEFFASSSAKDTDWVIKIMDVDLDGNSIKLADGILSARFRNSFYKSEFMEE
                     GEIYKFTIITSKISNTFKVGHKIRLDITSSAKNFIFQNSNTKEGYNSIEYIEAKNTIY
                     HGGKYPSKLILPIENK"
     gene            complement(15566..15910)
                     /locus_tag="PC700_001869"
     CDS             complement(15566..15910)
                     /locus_tag="PC700_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDF2581180.1"
                     /translation="MEIKRYEGTGRMSRAVVHNNTVYLCGQTHAEGGVIEQTTEVLAK
                     IEDLLNKYGSDKQHLLSVTIYLRDMKDFEAMNSVWDAWVEKGFEPARACVEARLAREH
                     LLVEMSVVAATK"
BASE COUNT         5875 a         1943 c         2742 g         5417 t
ORIGIN      
        1 actcttttat ctagttttat tttctttata gaacaataat attataaatg ctagtagatt
       61 tacacagaat actgttatat acatctgttt gaatcctgag tttagagtag attgtagtgt
      121 attttctatt tttgttctat tattttctat ggaatttata taatccaatt ttgatttatc
      181 aaaagccttt ggtatttcag tttttacctc ttgtgtcttt gctaaaactg cttggagtga
      241 ttttttttgc tgttgcattg tatcaaggct tttttccatg tctttctttt ggtttataga
      301 attatttaac ttgctttgta gctctttctt ttgagattcc attgatctta gttttttact
      361 taattcgttc ttttgagttt tatttttttg taactttgta tttaagtcat ctcttgtctt
      421 ttttagttgc tgtatttgag catttaaact ttctttttgt tcatttacat ttggtttatt
      481 ctcgctggaa ttgttattct tattttctgt agaatcattc ttagtgtcgt ctgattcaga
      541 attactggta ttactctggt tttgttcatt tgagttattt tcgttatcag taggagtctt
      601 ttctgtagta ggtattttat tataagcagt tgtaagttcc ttgatggcct catcttgttt
      661 ttcaagacct tgttccatac cttttatacc tttgtcaaca ccagcaatac ctttttttac
      721 accatctatt ccttgattaa taccagctct agcttttacc atattgtcaa taccagtttg
      781 aactccttta ataccactat ttagtttatt ttcaccatct tcaataccgt ttattccact
      841 ttgtgttcca tctattcctt tttgtacatt tttctgtatt ttagtaatta tagatggaac
      901 tttttcatca aacattgttg ttgatatttc ttttgttata ttggtaatgt tggttgtatc
      961 tgctgactga actttattaa gtaaggattt tggaagtttg ccacctgtca tgtccatatt
     1021 tatatttgaa gtatcattca tatttgggat agagacattt tttaaatttt ttgccatttc
     1081 agggtctgat tttaagtcat ctatcatgtt ttcaagttct tcaagttgaa ctattttagg
     1141 agttgagggc tttccaacta agtccataat attatcttgc acagataatc ctgcatgtgc
     1201 tataaatcca atcattatag ctggagaaat tgaagtacca attgagcgta tcaaggataa
     1261 tgttgcaagt gctgaattag attcttctaa tcttgtattt gataacatca tatagtttag
     1321 aggagttccc atagtaaagc ccattcctaa tcccataaat attaaactta cacatacact
     1381 aaatacagta ttggttttta gagcaattaa aactaggtaa agagagccta tcatagatac
     1441 agaaaatcct agtaatagta ttttctttgc cccaaacttg tcaataagtc ttccgcttaa
     1501 aggagcagca atacctgcaa ataatcccaa tatagctaca aaatatccac cagaaccagc
     1561 atttatcttt aatgcatttt cagcatactg tggaacgaat accataccca tcattccaat
     1621 accaacaatt aaagataaaa taagtactat caatattctc ggatttaaaa aatattcaaa
     1681 gcttaaaata gggtctttag ctctattttc aataaatatg aaaataggta taagtacaat
     1741 aaatgtcaat aagtatggat aaactgatat atcttgtata gaatttttaa aattaaaaaa
     1801 gtctatattc ataagaccat ataatagtga tacgattata catatcaaca tcaatgtacc
     1861 aagcttatct attttttctt tagattcaga cttattgttt ttaatacaga aaactccacc
     1921 aataagtatg attaaacaaa ttggaaggtt tacaaaaaat agccacttcc aattttgagt
     1981 tccaaagata cttagaattg tgctaccaat gcttgaacct aaaatattgg caataccata
     2041 tgttgcccca actaaaccta aagccatgcc tcttttattt tctgggaagg tagttccaaa
     2101 ttctgctgta gcaattggca tgattccacc accaccaatt gcttgaataa cacgaccaat
     2161 taataaaata tagaaattgg aaaatagtga tgataatcca catattaatg aaccactacc
     2221 aaatagaaat atactaacta gatacacata ttttcttcct attttatctg ctaattttcc
     2281 agagatagga attacagaag cgtatgttaa tgtgtatatg gtaatcatcc aaattcccgt
     2341 tttttcattt actccaaggc tattttgtat aatcgttctt gctggtgata ctattccagt
     2401 atcaatagct cccataaata tacctaatag ataaagtgta agtactaaaa atatttttgg
     2461 attactgtcc ttgttcttaa tactagtatt atgattcaag gtaaacacca ccttaatgta
     2521 ttaatttttc aatatagggt tttaaatctt caaattctat atgaagatat gttgaatatg
     2581 aaaaaaacat ttttattatg atattgcata ctaatttgtc attattaaaa aaactttctt
     2641 tttctaaagc ttcatatact ttatttgaaa gctgttcatg aaagtcgtct ttgtgttttt
     2701 tcatttcaaa aaatctctct gtttcaaaag tgtttaaaaa gtcattgtat tggatattat
     2761 gaactttgtt tataaaattt cttatttcag taaaaataat gtttagtttt tttatgtaat
     2821 cttcagtgtt tttattggag tattcaattc gccttatcaa taaatcccat tcagattttg
     2881 tgatatattc tattatttct tgttttgaat taaagtaatt atatactgta cctgcactta
     2941 taccacacct ttgagctatt ttccttatgc taatattcga gtaatcttct tctaaaatca
     3001 tatcccgaga tacttttaat atatcttctt ttacattttc taaaatcttt ggcatattac
     3061 cacctctttt attaaaatta ttatgaacac tgtttattat actcctttaa aatttattaa
     3121 tcaatatgta agctataatg tataatatta ttatatagat atgatagaaa ataagtgtct
     3181 ttttataaat atctattata ttaaatttgt acttaagtat gattatttat tgaaatatgt
     3241 gatattaaat ttttcttttt aataaattaa atattatatg ataaggagaa tatggtgatg
     3301 gaaaagaagt atgtaatggc attagaccaa ggaacaacca gttcaagagc tatactattt
     3361 aataaaaaag gcgaaatagt gaaaattgca caaaaagagt tcaatcaaat ttatccaaaa
     3421 gctggatggg tagagcatga ccctatggaa atatgggggt cacaaagtgg tgtaatgaga
     3481 gaagttatag aaacagcagg tataagacca gaagaaattg cttcaatagg gataactaat
     3541 caaagagaga ctacggttgt ttggagtagg tatactggta aacctattta taacgcaata
     3601 gtatggcagt gtaggagaac atcagaaata tgtgatgaac ttaaaaataa aggattggaa
     3661 gaatcaataa aagaaaaaac aggtctttta atagatgcat acttttcagc aacaaaagta
     3721 aaatggatat tagataatgt agaaggagct agggaaaaag cagaacaagg agagttatta
     3781 tttggaacta tagatacatg gttaatatgg aatttaactc gtgggaaagt acatgtaaca
     3841 gattactcaa atgcatctag aacaatgatg tacaatataa atgatttaga atgggatgag
     3901 gatatattac gagaattgga cataccaata agcatgttgc cattagtaaa accttctagc
     3961 tatgtttatg gtcatacaga tgagcgcatg ttatcaggag ctaaaatacc tattgcagga
     4021 tgtgctggag accaacaagc agcattattt ggtcaaaatt gtgttgaaga aggtactgca
     4081 aaaaatactt atggaacggg ttgtttctta cttatgaata caggcagtaa catagttaaa
     4141 tctaagcatg ggttacttac aacaattgct tggggagttg atggaaaagt aacatatgca
     4201 ttagaaggaa gtatatttat tggaggagca tctgttcaat ggcttagaga tgaattaaag
     4261 ataattgaat ctgcaaaaga tagtgaaatg tatgcaaata gggtagagga tactaatggt
     4321 gtatatgtag tgccagcatt tacaggtctt ggagctccat attgggatat gtatgctagg
     4381 ggttcaattt taggacttac aagaggagca aaaaaagaac atatagtaag agccacattg
     4441 gaatcaattg cttatcagac aaaggatgtt ttagaagcaa tgcaaaacga ttcaaaatta
     4501 aaactaaaat cattaaaagt tgatggtgga gcaagtaata ataatttttt aatgcaattt
     4561 caatcagata tattaaatgt agatatagat agacccaaaa tagtagaaac tacagcttta
     4621 ggagctgctt atttagcagg gttgtctgtt ggattctata ttggaagaaa tgaaattact
     4681 tcaaagtggt cagtagaaaa agagtttaat ccaaatatga gtgaagagaa gagatgtaaa
     4741 ttatataaag gatggaaaaa agctgtatcg agagctttaa gctgggaaaa agaagatgaa
     4801 ttggatattt aaaaattaat caaagttaaa tttatttgaa tttaaaaaga gctttatgta
     4861 aaatatgaag ctcttttttt gttgttaatt ttgcattttt atataatcat actaaattat
     4921 ataatttatt tttcgttttc atataaaaac attggtatag aattgtaaat gtgttggtca
     4981 ccaactacta agagaatgtc tccttctaaa aattcaatat atggtccagg agatattata
     5041 agttcttcac ctcttttaat tgcaactatt gtagcacctg tattttgcca aaatttagtt
     5101 tgtgcagctg tttgaccaat atatttgcaa ttagaattta tggtgaactg aacgggcact
     5161 aaaggattga ttatttcaag tctgttggaa tagtctaaaa ttttatttat agtatctaat
     5221 atttcatttt ctaaattaga tttagtatct attaaattat aaagtgttga tttatatgat
     5281 gatatatttg taatatctct atattttttt ataaaagaag aagctttgtc tggagatagt
     5341 acaataacac ctttaccctt tatgctttta acaacgccaa tgtcctctaa aattttgaca
     5401 gctcttctta tcgtttctgg agatacattg tatttaccag ccaaaacaga gcgtccaaat
     5461 agtactgaat cctcttgtat ttcacccgtg tatattttat ttgcaatatc caacgctatt
     5521 ttttggtata ttggcatcgt atagttttta tccattgttt ttcccccaat acaaaattta
     5581 tgttaaaaaa atatataatc ttaacaatac tattgtataa tttataggtt tatatgtcta
     5641 caatatgtac aaatattgtg catgttttaa aataatattt taacatgcat ttagtataca
     5701 tgattttatt atacttaaaa ctatataaag aattaattat aaaaatataa agatatttta
     5761 cttacaaaga atgataatta tatagatgaa agaaacaaat aaatgaagaa aaatgattct
     5821 gtcttgcata taaagtgatt aaaaatgaaa aatactttaa aaatgaaggt taaggccctt
     5881 attttaagtg aaaataatta attattaact aaaaaatggt aaaaagattt tttgaaaaag
     5941 aaatacaaat gaatgttcaa ttaaaataat tgcaaaaata ttccttaaat ggtaaaatta
     6001 tattaattaa atgcaataga atagttgcaa aaataaaatt gaatacaagt gataaaatat
     6061 ttcatgcaat atataaaata aacaaaattt aaaaattaaa actacttatt ctatcaaaat
     6121 gttagcaagg agggggtcat aaattgtata aagtagattt aaatagtgat ttaggagaaa
     6181 gttttggaac gtataagatt ggtttagatg aagaagtttt aaaatatatt tcatcagcta
     6241 atatagcttg tgggtttcat gcaggtgacc ctagtcatat ggaaaaaaca gtacagctag
     6301 ctaaaaaaaa tggagtgaaa ataggcgcac atccaggttt cctagattta ataggatttg
     6361 gaagacgtga gatgaaaata acaaagcagg aagctaaaga ttatacaaaa tatcaattgg
     6421 gtgctttaat ggcatttgca agttcaaatg gatgtaatat acaacatgta aaaccacatg
     6481 gagcattata caatatggca gctaaagata aagagttagc tatggggatt tgtgaagcta
     6541 tatatgaagt cgataaagat attatactgt tggggctata taatagtgaa atgataaatt
     6601 ctgctaaaga aattggactt aggtttgcaa atgaagtatt tgcagataga gcttatgata
     6661 ataatggttt tttggttcca agaagtgtag aaggagcagt tatacatgat acaaaacatg
     6721 caatagatag ggttgttagg atggtaaaag aaggaactgt agaaacttta acaggtgaag
     6781 ttatacatat caaagcagat tctatatgtg tacatgggga caatccaaaa gcaatagagt
     6841 ttgtcaaaga aataagaaag cgttttgaat tagaaggtat agaagtttgt ccattagaaa
     6901 acatagaagt ttgttcgtca gagaacatag tataagactg ataacatgag ggggagataa
     6961 aatatgagta caaaaaatat tacagataaa actaaaaata aaaaagatgt cggagcactt
     7021 ataggggctg catttataat ggcaacatca gcaattggac cagggttttt aactcagact
     7081 gcacaattta cccaagattt tggaccaaat ttttcatttg taattttaat tacaacaatc
     7141 ctttttatag gggctcaagt aaatgtatgg agagtaattg gtgtatcagg acttagaggt
     7201 caggatatag caaataaaat aataccggga ttaggttatt tagtagcttt tttagtagct
     7261 cttggaggat tagcttttaa tataggaaat gttggtggag ctgcacttgg tatgaatgta
     7321 atgtttaata tgaatatgac attaggaact gtattaagtg gattaatagc tatatttgta
     7381 tttatgtcaa aaaattcaaa ttctctagtc gataagataa ctaaattttt agcattagga
     7441 atgataatta tagttggata tgtagcaata tcaaatcatc ctcctgttgg agaggcagta
     7501 tcaagaatgg taaaaccaga aaatccaaaa ggattgatat tcccaataat aactctactt
     7561 ggaggaagtg ttggaggtta tataactttt gctggaggac atagattaat agacggtgga
     7621 ataacaggag aagaaaatat aaaagaaata actaaatctt ctcttttggg tatattagtt
     7681 gacacaatga tgagagttct tttattttta gcaatactgg cagtggtttc aaaaggtctt
     7741 caacttgacc cagaaaatcc agctgcttca gcttttaaat ttagtgcagg agcaatagga
     7801 tataaattct ttggacttgt attatggtct gctgcaatta catcagttat aggagctgct
     7861 tatacatcag tttctttctt aaagacacta aatcctttta tagataaata tgaaaaatat
     7921 tttataatag catttatagc aatatcaaca cttataatgg catttatagg aaaaccagct
     7981 actttattaa ttttagcagg agctttaaat ggattgatac ttccaatcac attaggaatt
     8041 atgcttatag catctaaaag aaaagatatt gttggagatt acaagcatcc tacatggctt
     8101 ttaatatttg gattaatagt agttttaata tctgcatata caggaataac ttctcttagc
     8161 agcctaggtg ctttatttgc ttaatttaga agaggtgaca tttatggaaa ctagatactt
     8221 gatatcaggg gataaggcag tggttgctga atttggaaat gagatatcag aagatataaa
     8281 caaaaaagta atttcattta tgagagcaat agaaatatcg aatcttaaag gtattgtaac
     8341 agaaatggta ccaacatata ggtcacttat gataagttat aatccattag aaatagattt
     8401 tgatagtttg attgagaatt taaaaaaaat agaagataat ttggaaagta tagtattacc
     8461 aaaacctaaa atacatgaga ttccagtatg ttatgatgag gtttttggta tagatattaa
     8521 aaacgttgca agctacaata atctcactgt agatgaagtt ataaaaatac acacaagcag
     8581 agagtacttg atatatatgc tagggtttac tcctggattt ccatatctag gtggtatgga
     8641 tgaaagaata gctacaccta gattagaagt gccaaggact aaaatttatg gtgggagtgt
     8701 tggaatagca ggttcccaga ctggtgtata tcctattgat agtcctggtg gatggcaaat
     8761 aattggtagg acacctttaa aattatatga tgaaaataga gaagaacaga ttttgcttag
     8821 agctggagac tttataaaat ttgttccaat aacattagat gaatttatag aaatcgaaaa
     8881 aaataactta agtatttaaa tatcgttata gtacagggag tggtatatat gggattcaaa
     8941 gttgaattag gaggtttcca aacattaata caagacagag gtagagttgg ctatggtcaa
     9001 tatggtgtat ctggatgtgg agcaatggat gagtatgcac atagagtagg aaatatatta
     9061 gtaggaaatt ctgaagatga ggcttcttta gaagttttaa tgttaggtcc tacaattacc
     9121 tttgatgaat atacgcaaat agcagtaact gggggagatt taggggctaa aataaatgga
     9181 aaagaaattc aaaactggag gtcatatcaa ataaatccag gtgatgtatt atcatttaga
     9241 ggtgtgaaat caggtgctag agcctatgtg tcaatagctg gtggaataga tgttccactt
     9301 gcaatgggaa gtaagtcaac ttatacaaga tctaaaattg gcggatttga aggtagagca
     9361 cttaaaaaag gagattatat aaatactttt attcaggaaa aagattttac aataaataaa
     9421 aaattaaatt caaaatatat tccaacttat agttcagaaa tcgtactaag aattgtaaaa
     9481 ggtcctcaat ttgatgcttt ttctaacggt gaagtagaga aatttttatc aaataaatac
     9541 aaagttacga atgaaataga tagaatgggt tgtagactag atggagagag tataaagcac
     9601 cttaatggag cagatattat atctgatggt atttcttatg gagctataca agtacctggt
     9661 catggaaaac cgattataat gttatcagat agacagacta gtggtggcta tactaagata
     9721 gggaacgtta taagtgtaga tttgtataag ttagcacaag caaagccaaa tgatgtagta
     9781 aaatttgaac ttgtagatat atatgaagca catagactat tgagagagca agaagataag
     9841 attcaagata tctacaagag tatgaaaaat ataagagttg taaaggcaaa agtattaaat
     9901 gatatagcag tttaatttaa ttttaacaaa tacaattttt attataattt ataatataac
     9961 ttacaaatag ctatttacta tttaccataa ttaagatggt ttagattagt caaatagcta
    10021 tttttttagt ttgagtaaat attttaaatt taaataaata cttcaatttt aaaataaaag
    10081 caatatttaa atttattata ttatctacaa aaagtaggtg tgttatgttg agtttattta
    10141 gattttattg gccaaattgg aggtgatata actccatatc cacgagctat tgtatcttta
    10201 cttggattga tgattttatg tggtacatta gcttttataa atgttgagtc acctggattt
    10261 aatctaacta ccttttcacc catacaaata tcaaccacac cactcgttac caccataatt
    10321 tcttctgatg catgatagat ataatcttta gcatcctcac tttctggttt aagttcaaac
    10381 tctacaaata aaattgaagg tgtaaattca gttgtaggca ttggagatac tatctcgtaa
    10441 aacatttcac tctttggaaa tttcatcata actctatctt cttttctaat tactaaatca
    10501 tcagtattat tgtcatccat aaacatatat aaaggagtgt ctaaaacttc agcgattttt
    10561 ctcaatgaag acaaggatgg ttcaatagca tctctttcta tttgagagat ataacttata
    10621 gataaatctg tcaatttgga aacatcactt aatgtttttt tcttgctttt tcttatattt
    10681 ctcatctttt cacctagcat aagaacctcc aaataataaa attaatataa aatattcgct
    10741 taaaacaaag taattatagc acaacataga agtagtggca ataagaatac atagtagatt
    10801 ttgacaatta aaatttaatt aaatccaaat aaaagtctat gaaaatgaat tattgttaaa
    10861 ttaatataaa gaaaataata ataaaaaaat ttaatagaag agtattaatt gaattaagat
    10921 tatcacatta tattttggaa aagagtaaat tttcagtaaa ttatcttgac atatatattg
    10981 aagtaataat ataatttaaa gtataaaatt ttaaaaattg aattataact tcaaaaaata
    11041 aggagggtaa tatgaagata acagatatca aatttgaaaa gcttagaatt aaattaaaaa
    11101 aacctgtagt agtttcattt ggagtaattg aatatggaga aagtgtaatt ttaaaaattg
    11161 aaactgatga agggtattgt ggatttggcg aagcagcacc tcttgcagca gttacaggtg
    11221 aagttttgga taatgttctt tcagtattgc ttatgtttaa aaaagagtta ataggcaaag
    11281 acccactaga tattgaaacc atacatacaa tcatggatgg aattattata ggtaatactt
    11341 ctgcaaaagc agctattgac atagctttat atgatattaa aggaaaaatt atgaatgcac
    11401 ctctttataa agttcttgga ggatttgata gtaaagtcca aacggatatt actataagta
    11461 tagataagcc agaaaaaatg gcagtagaag ctttagaaag agtaaaagaa ggttttagga
    11521 tattgaagct taaagcaggt ataaatcctg aagacgatat tgaagctgta aaattaatta
    11581 gagaagccgt tggagagagt ataagaatta gaattgatgc aaatcaagga tggaacgtaa
    11641 atagcagtat aaatactatc aaaaaattag aagaatttaa tgttgatgca attgaacaag
    11701 cacttccaca ttgggattta gatggtactg cttacataag aaataaaagt aatacaaaaa
    11761 ttatgataga tgaatcgtta cattcaccaa tagatgcaat taaggctata aaaaagaatg
    11821 cagttgatac atttaatatt aagctgatga aatcaagtgg aatatatcct gctataaaaa
    11881 tcaacaacat agctgaggca agtggagtaa attgtatgtt aggatgtatg ctggaaacga
    11941 gaattggtat aacagcagca gcaaatttaa tagcatctaa aaagaacata acagaagctg
    12001 accttgatag ttttatgttt tgtgaagaat caaaagatat atctggagga tttgtaatgg
    12061 atggagatat tatgaatctt gtcaataaac caggtttagg aatagaagta aacttatgat
    12121 aaatttggag gttaaatatg aatacacttt tttcaaatcc aggtagtata cttgctgtaa
    12181 tgacatcaat gattgcattg ggtttttact tacaaagata caaagccatt aaaagcttag
    12241 gaccagcact tacaataatc ataatgggaa taatactttc aaacttaaaa gtagttccag
    12301 taagtactga gttatatgga actatttcaa catatgctat cccagtttct atgactataa
    12361 tgttgatgag tgttgactta aaagaaatga caaaattatc aagagaacct ttaattgcaa
    12421 tttttgtagc tgtattaact gtaagtatca tggcattttt atttggatta gtatttgcag
    12481 aaaaaatatc agaaggttgg aaggttgctg gaatgtttgt aggtacatac actggtggaa
    12541 gtgctaatct tacagctatt ggaacaggcc ttaatgtaag tagacaaact cttgctgcag
    12601 caaatgcagc agactatgta attggagtac caacattgat atttatgttt gctctaccag
    12661 caatattaaa aaattcaaag aagtttaaaa aactttggcc atatcatgta gaagaaacag
    12721 aattagaaga ttgtcaaaat gaagaattta tggagtcaaa agaatggagt attaaagata
    12781 tagcttggat gttggctata ggttttgttg ttacagaagt ttcaacaata cttgctggat
    12841 atttcaattc aagttttagt agtgcagcaa gaatactttt agttacaaca atttcaatta
    12901 taattgctca attaaagcca gtaaagaaac taaagggaaa tttagattta ggattatttg
    12961 ttgcattatt ctttttatgt acaataggtt tttcagtaga tataaaagag tttttaggtt
    13021 caacttttac tataacactt tactgtttta gcataatttt tgcttcattt gtttttcatt
    13081 taggcataac tagactttta aagataaagt atcaatatgt aatactttct atagtaggag
    13141 caattgctga tggtcctact tcagctttag tagctgcttc tgcaaagtgg aattcgcttg
    13201 taagtgtagc agtcgtaatg ggagtaattg gtggagtatt aggaaattat gcaggtatat
    13261 cagtggcata tgcaataaag atgtctctag gattataggg gggagtttaa ttgataaaat
    13321 ttaaattata catatctgga ctatacagtg gaaatgtaat ctttgatggc gatttattaa
    13381 ttgaaaagtt gaatcctttt acaaataaaa tagaaagttt aaaaccaata agcaaagaag
    13441 aaaatacata ctatttaaat cttacaaaaa tagatttaaa gtctttattt aataactttg
    13501 atgtatatac taaaagctta gtaaacacag ataaagacac agttataaat aacttaggag
    13561 aaacgcattc taaacttaat gaatatatat gggttcaaag aaacaaaaag ttcccactag
    13621 atataattat tgtagataat aaaatagtag gttttatctg tttatccaga gaaacttgta
    13681 ctatattaat tatggatgga tatgaagagt atactgtatt aaaagaatgg gaaaaaaccc
    13741 ataaaaatga agagatatat tctataagat ttggtggaaa ttatatgatt gatatgaaag
    13801 atggtataaa actttctaca gatgtatatc tccctgattt tgtagattct accaaaaaag
    13861 caccaacaat tcttatgaga actccatatg gaaaagaaaa tgataaagaa atatattata
    13921 aatatgtaca aagaggatat gctgttgtaa tacaagatgt taggggaaga aatgaatcag
    13981 aaggtaagtg ggaacctatg attcatgaga gggaagatgg agatagtacc ataaattgga
    14041 tagtatcaca ggaatggtca agtggaatag ttggaatgct tggagcatca tatcttggtt
    14101 atgtccaatg ggcagctgca tcttcaggca ataagcatct taaagcttta gttagtatag
    14161 taacatcagg aagccctttt attgatatac caagaaaagg aggagctttt gtatctggta
    14221 tgcttgcatg ggcatttatg gtatcaagaa ataaagttga tagaagtaaa atggtaagag
    14281 atgattggga tgatgtttta aacatacgac caatagaaaa tatacctgtt gaagctcttg
    14341 gatatagaat tgaatttctg gaagaatggt taaagagagt agaaaaagat gagtattggg
    14401 atttgatgga ttggcattta caaaaagata aaataaatgt acctgcattg gttgtatctg
    14461 ggtggtatga tgataatagt atgggaacta cagaggcctt agatgttata aaggactatg
    14521 aaaaaggaaa gagaaaggct attctaggtc catggatgca taattcaaat actttaagag
    14581 atataaatgg tatttcttta ggaaatagtt cattaagata tgatttggat tataattatc
    14641 tattatggtt tgataaatac ttaaaaggta tagaaaataa catagatacg actgctccag
    14701 tagagtatta ctctgtaggt tttaataagt ggaagacaga agaaaattgg ccaataataa
    14761 ataagataga taaaagtatg tatctaataa gtgatggaaa tgctaacaca tctttaggaa
    14821 atggtagatt agtatttgat aatgatttag aagaaaagta tgatagttat atatataatc
    14881 caaaagaccc atcagtacaa ctaattgata tgtcagagaa tgaagttgga gttccaaata
    14941 actacaaaga tttagaaaaa agaagtgata tgttgtgtta tacatcagat gcttttagtg
    15001 aagaatttac tgtaacaggt gatataaagt tagagttttt tgcatcaagt tctgccaaag
    15061 atactgactg ggttataaaa attatggacg tggatttaga tggaaattca attaaattag
    15121 ctgatggtat acttagtgct agatttagaa atagttttta taagtcagag tttatggaag
    15181 aaggagagat ttataagttt acgattataa cttcaaaaat atcaaacacg tttaaagttg
    15241 gtcataaaat tagactagat ataacgtcta gtgctaaaaa ctttattttc caaaatagca
    15301 acacgaaaga gggatataat agtattgaat atatagaagc aaagaatacg atttaccatg
    15361 gaggtaaata tccatctaaa ttgatattgc caatagaaaa taagtaaatt tatatagatt
    15421 aaatagtgag gaggtgtctc aaaatgaatt ttttagttcg tgagacactt tcttttatac
    15481 gaaatcaaat atattttcat catttcaaca atgaaaaaga ggcttatctc tctgttttga
    15541 gatagcctcc tataagctaa attgattatt ttgtagctgc aacaacgctc atttcaacta
    15601 acaaatgctc tctagcaagt ctagcttcaa cacaagctct agcaggttca aatccttttt
    15661 caacccaagc atcccaaaca gagttcatag cttcaaagtc tttcatatct cttaaataaa
    15721 tagtaacaga tagcaaatgt tgcttatctg aaccatattt gtttaataaa tcttctattt
    15781 tagctaaaac ttcagttgtt tgttcaatta ctcctccttc agcatgagtt tgtccacata
    15841 aataaactgt attgttatga actacagctc tactcattct tccagttcct tcatatctct
    15901 ttatttccat attttaaata cctccatagt taagaaatct attaaaaatc taaataataa
    15961 attaaaaaaa gtctata
//
LOCUS       DAMJWP010000071        15959 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.075, whole genome shotgun sequence.
ACCESSION   DAMJWP010000071 DAMJWP010000000
VERSION     DAMJWP010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15959
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.075"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            235..384
                     /gene="rpmG"
                     /locus_tag="PC700_001870"
     CDS             235..384
                     /gene="rpmG"
                     /locus_tag="PC700_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004402360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDF2581181.1"
                     /translation="MRVKVTLACTECKQRNYNTTKNKKNNPDRIELQKYCRFCKKHTT
                     HKETK"
     gene            410..631
                     /gene="secE"
                     /locus_tag="PC700_001871"
     CDS             410..631
                     /gene="secE"
                     /locus_tag="PC700_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDF2581182.1"
                     /translation="MAAQKNDGAKTKKRFSLFGYLKETKQELKRVTWPTKKELFKNTS
                     IVLTVVISCTILVWGIDTILSGALALLLK"
     gene            651..1193
                     /gene="nusG"
                     /locus_tag="PC700_001872"
     CDS             651..1193
                     /gene="nusG"
                     /locus_tag="PC700_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination factor
                     NusG"
                     /protein_id="HDF2581183.1"
                     /translation="MSELQEASWYVVHTYSGHENKVKATIEKAVKTRGMEDCIRQVVV
                     PTEEVVETTKTGKEKTRQRKVYPSYVLVKMIITDESWYVVRNTKGVTGFVGPGSKPVP
                     LSEDEVKAMGIDTTDPKVVNSDVDFEIGDTVKVSQGPFSGQIGNIEEIDLENREVKVC
                     INAFGKRTLFVIELEGIEKI"
     gene            1229..1654
                     /gene="rplK"
                     /locus_tag="PC700_001873"
     CDS             1229..1654
                     /gene="rplK"
                     /locus_tag="PC700_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDF2581184.1"
                     /translation="MAKKVIGQIKLQIPAGKATPAPPVGPALGQHGVNIMGFTKEFNA
                     KTADQAGMIIPVVITVYQDRSFSFITKTPPAAVLIKKALNLKSGSGEPNKKKVAKMTS
                     AQVREIAELKMPDLNAASVEAAMSMIAGTARSMGVVIED"
     gene            1724..2422
                     /gene="rplA"
                     /locus_tag="PC700_001874"
     CDS             1724..2422
                     /gene="rplA"
                     /locus_tag="PC700_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDF2581185.1"
                     /translation="MAKKGKRYAGALQKVDRTKFYDASEALTLVSDIAGAKFDETVEA
                     HIKLGVDSRHADQQVRGAVVLPHGTGKTKRVLVFAKGEKAKEAEQAGADFVGAEELVQ
                     KIQGENWFDFDIVVATPDMMGVVGRLGRVLGPKGLMPNPKSGTVTFDVAKAIDEIKAG
                     KVEYRLDKTNIIHVPVGKVSFGGEKLTENFTALMDAIIKAKPAAAKGQYLRSITVASS
                     MGPGVKINPARTAE"
     misc_feature    2474..2602
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            2645..3151
                     /locus_tag="PC700_001875"
     CDS             2645..3151
                     /locus_tag="PC700_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDF2581186.1"
                     /translation="MRKAIEIKSEVVSEIVEKLQKSSAAVVVDYKGLTVEEVTELRKQ
                     MREAGVDYKVYKNTLVRRAAKEVGIEQFNDELLVGTNAIAFGYDDPVAPARILKGFMD
                     SHPKMKLKMGIVEGAFYDESKIVEMANIPSREVLIAKLLGSLKAPVSNFAYLIDAIAK
                     KAEGQEEA"
     gene            3209..3574
                     /gene="rplL"
                     /locus_tag="PC700_001876"
     CDS             3209..3574
                     /gene="rplL"
                     /locus_tag="PC700_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDF2581187.1"
                     /translation="MTIEQILEAIENMKVLELNELVKAAEEKFGVSASAPVMVAGAAA
                     GGPAAEEKTEFDVVLTDVGSSKVGVIKAVREITGLGLKEAKEVVDNAPKTVKEGASKE
                     EADQIKEKLEAAGAKVEVK"
     gene            3966..4754
                     /locus_tag="PC700_001877"
     CDS             3966..4754
                     /locus_tag="PC700_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDF2581188.1"
                     /translation="MEKLQGKIAVVTAATKGIGLASAEILAKNGATVYLAARSEELAN
                     EVINKISAEGGCAKFVYFNAREEETFTSMIEEVVKKEGKIDILVNNFGSTNPSLDKDL
                     VTGDTDNFFDTVNTNLKSVYLPCKAAIPHMIKNGKGSIVNISSIGSVLPDLSRIAYCV
                     SKAAINSLTQNIATQYAKDNVRCNAVLPGLIATKAALDNMSPEFIKEFLKHVPLNRIG
                     EPDDIAKAVLFYASDDSSFITGDLLEVAGGFGLPTPQFADNILG"
     gene            5225..8941
                     /locus_tag="PC700_001878"
     CDS             5225..8941
                     /locus_tag="PC700_001878"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDF2581189.1"
                     /translation="MPHPVTIGKRTRMSFSKIKEIADVPNLIEIQVDSYEWFLKEGLK
                     EVFDDISPIEDYTGNLILEFVDYSLDDKPKYDIEECKERDATYCAPLKVKVRLINKET
                     GEIKEQEVFMGDFPLMTERGTFVINGAERVIVSQLVRSPGVYYAEERDKTGKRLISST
                     VIPNRGAWLEYETDSNDVISVRVDRTRKQPVTVLLRALGIGTDAEIIDLLGEDERLSA
                     TLEKDNTKTVEEGLVEIYKKLRPGEPPTVESASSLLNALFFDPKRYDLAKVGRYKFNK
                     KLALCYRIMNKISAEDIINPETGEVFVKAGEKISYDLAKAIQNAGINVVNLLMDDDKK
                     VRVIGNNFVDIKSHIDFDIDDLNIKEKVHYPTLKEILDGYSDEEEIKEAIKSRIKELI
                     PKHILLDDIIASISYEFNIFYNIGNIDDIDHLGNRRIRSVGELLQNQVRIGLSRMERV
                     IKERMTVQDMEAITPQALVNIRPVSAAIKEFFGSSQLSQFMDQTNPLSELTHKRRLSA
                     LGPGGLSRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLINSLGTYAKINEFGFIESP
                     YRKFDKETSTVTDEIHYLTADEEDLFVRAQANEPLTEDGKFVNHRVVCRTVNGAVEMV
                     PESRVDYMDISPKQVVSVATAMIPFLENDDANRALMGANMQRQAVPLVRREAPIIGTG
                     IEYRAAKDSGAVVVARNSGIAERVTADEIIIKREDGNRDRYNLLKFKRSNSGTCINQT
                     PIINKGDQIIKGDVIADGPATDLGEVALGRNCLIAFMTWEGYNYEDAILINERLVKED
                     RLSTIHIEEYECEARDTKLGPEEITRDIPNVGDSAIKNLDDRGIIRIGAEVDSGDILV
                     GKVTPKGETELTAEERLLRAIFGEKAREVRDTSLKVPHGESGIIVDVKVFTRENGDDL
                     SPGVNELVRCYIAKKRKIKVGDKMAGRHGNKGVISRVLPEEDMPFMENGTPLDIILNP
                     QGIPSRMNIGQVLEVHLGLAAKTLGWYVATSVFDGANEYDIMDALEEAGYPRDGKLTL
                     YDGRTGQSFDNRITVGYMYYLKLHHLVDEKLHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAHILQEILTVKSDDVVGRVRTYEAIVKGENIPEPGIPESFKVLIK
                     ELQSLCLDVKVLTDEDQEIEVRESVDEDDTIGEFELDVVNHMGEVEESNIIEEIEDDF
                     VEATEDEDIENLEEFTEDDLFEEEIDFESDDFDM"
     gene            8983..12468
                     /gene="rpoC"
                     /locus_tag="PC700_001879"
     CDS             8983..12468
                     /gene="rpoC"
                     /locus_tag="PC700_001879"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDF2581190.1"
                     /translation="MFELNNFESIKIALASPEKIRQWSRGEVKKPETINYRTLKPEKD
                     GLFCERIFGPQKDWECHCGKYRRVRYKGVVCDRCGVEVTKSKVRRERMGHIELAAPMS
                     HIWYFKGIPSRMGLLLDMSPRSLEKILYFASYVVVDPGETGLNEKQLLTEKEYRTALE
                     KYGYTFTVGMGAEAVKTLLQNIDLEQQSKDLRAELKDSTGQKKVRTIRRLEVVEAFKK
                     SGNKPEWMILDAIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELGA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKFYQCGLPKKMALELFKPFVMDKLVKEGYAHNIKSAKSIVEK
                     VKPEVWDVLEDVIKSHPVLLNRAPTLHRLGIQAFEPILVEGKAIKLHPLVCTAYNADF
                     DGDQMAVHVPLSVEAQAEARFLMLSVNNILAPKDGSPITTPSQDMVLGCYYLTIEAQD
                     GAKGTGMVFKDFNELLLAYYNKSVHLHALVKLKVTLEDGRSSLVESTVGRFIFNENIP
                     QDLGFVDRKENPFALEVDFLADKKSLGKIIDKCFRKHGNTETAELLDYIKALGFKYST
                     LGGITVAVDDMSVPEEKKVFIAEAEAKVDKYEKAYRRGLISDEERYEKVIETWTETTD
                     KVTDALMGGLDRLNNIYIMAHSGARGSKNQIRQLAGMRGLMANASGKTVEIPVKSNFR
                     EGLSVLEYFTSSHGARKGLADTAIRTAESGYLTRRLVDVSQDVIVREIDCGTEDTTEI
                     YAIKEGNEVIEEIYDRIVGRYTIDPILNPETGEVLVEADSMIQEDEAETIVALGIEKI
                     RIRTVLNCKTNHGVCSKCYGRNLATGKEVNIGEAVGIIAAQSIGEPGTQLTMRTFHTG
                     GVAGADITQGLPRVEELFEARKPKGLAVITEVSGRVEIDETGKRKEVNVIPEEGETQT
                     YVIPYGSRLKVKQGQMLEAGDPLTQGFINPHDIVRVNGVKGVQEYIVKEVQRVYRLQG
                     VDVNDKHIEVIVRQMLSKVKVEDPGDTDLLPGGYEDVLTFNECNKDAIDKGLRPAVAK
                     RVLLGITKASLATDSFLSAASFQETTRVLTEAAIKGKEDHLIGLKENVILGKLIPAGT
                     GMKKYRNIAVEKIED"
     gene            12757..13179
                     /gene="rpsL"
                     /locus_tag="PC700_001880"
     CDS             12757..13179
                     /gene="rpsL"
                     /locus_tag="PC700_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDF2581191.1"
                     /translation="MPTINQLVRKSRKALEKKSTAPALQKGYNSLNKKVTDASAPQKR
                     GVCTSVKTVTPKKPNSALRKVARVRLTNGIEVSAYIPGEGHNLQEHSVVLIRGGRVKD
                     LPGVRYHILRGTLDTAGVDKRRQSRSKYGAKRPKEAKK"
     gene            13302..13772
                     /gene="rpsG"
                     /locus_tag="PC700_001881"
     CDS             13302..13772
                     /gene="rpsG"
                     /locus_tag="PC700_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDF2581192.1"
                     /translation="MPRKGNIPKREVLPDPMYGSKVVTKLINNLMVDGKKGKSQRIVY
                     DAFAIVAEKTGEEALEVFNKAMDNIMPVLEVKARRVGGANYQVPIEVRPERRQTLGLR
                     WLVKYTRARGEKGMVEKLAKEIMDAANNTGASVKKKEDTHKMAEANKAFAHYRW"
     gene            13821..15887
                     /gene="fusA"
                     /locus_tag="PC700_001882"
     CDS             13821..15887
                     /gene="fusA"
                     /locus_tag="PC700_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDF2581193.1"
                     /translation="MARKFPLEKTRNIGIMAHIDAGKTTTTERILFYTGQTHKIGETH
                     EGASQMDWMEQEKERGITITSAATTASWKDHRINIIDTPGHVDFTVEVERSLRVLDGS
                     VAVFCAKGGVEPQSENVWRQAETYGVPRIAFINKMDILGADFYNVISMMKSRLNSNAV
                     PMQLPIGKEDSFIGIIDLLKMDAVIYKDDLGVEMEETDIPEDMKELAAEWREKLVESV
                     AETDEELMMKYLEGEELTIDELKVAIRKATIACEMNPVFCGTAYRNKGVQLVIDAVLD
                     YLPAPTDIPAIKGILADGEEAERHSSDEEPFSALAFKIMTDPFVGKLAFFRVYSGTLE
                     SGSYVLNATKNKRERIGRILQMHANTREEITKVYAGDIAAAVGLKDTTTGDTLCDPAN
                     PIILESMEFPEPVISVAIEPSSKAAQEKMGIALQKLAEEDPTFTVKTDQETGQTIISG
                     MGELHLEIIVDRLLREFKVEAKVGAPQVAYRETITQPVDVEYKYSKQSGGRGQYGHVK
                     IRVAPQEPGEGYKFTNKTVGGSVPKEYVGPVDMGIQGAMQSGIVAGYPVVDVAVELYD
                     GSYHEVDSSEMAFKMAGSMAFKDAMKKGNAVLLEPYFKVEVVTPEDYMGDVMGDLNSR
                     RGLIQGMEARSGAQVINAFVPLSEMFGYSTDLRSSTQGRATYTMIFDHYEQVPASVAK
                     KIAEGK"
BASE COUNT         6246 a         1985 c         3249 g         4479 t
ORIGIN      
        1 atggattaaa aggaataaca aaaagagggg ttaggctcct ctttttttaa catagaaata
       61 caaaaacatt gacacagcat aggattttgt gataacttat acaaggtaat gattatagga
      121 agttaataat atcttgaata ctgtagattt ttaataaata catattttaa agatattata
      181 ttcacatata ttaacaatat atattattgt attacaaagt tggaggtgta tcagatgaga
      241 gttaaagtaa ctttagcatg tacagagtgt aagcaaagaa actacaacac tactaagaat
      301 aagaaaaata atccagacag aatagaatta caaaaatatt gtagattctg taaaaagcat
      361 acaactcata aagaaacaaa atagtttttt ataaggatgt gagtatggaa tggctgccca
      421 aaaaaatgac ggtgcaaaaa ctaaaaaaag gtttagttta tttggatatt taaaagaaac
      481 taagcaagaa ttaaaaagag ttacatggcc gacaaaaaag gaactgttta aaaacacaag
      541 tatagtttta actgtagtta tttcatgcac tatattagta tggggtatag acactatatt
      601 atctggtgca ctagcgttat tactaaaata gacgaaaaga ggtaagttaa atgtcagaat
      661 tacaagaagc aagttggtat gtagttcata cctattcggg acatgaaaat aaagttaaag
      721 ctacaattga gaaagcagtt aaaacaagag gtatggaaga ctgcataaga caagtagttg
      781 ttccaacaga agaggtagtg gaaactacta aaactggaaa agaaaaaact agacaacgta
      841 aagtttatcc aagttatgtc ttggttaaaa tgataataac tgatgaatct tggtatgtag
      901 taagaaatac taagggagtt acaggtttcg ttggacctgg ttcaaaacca gtaccattaa
      961 gtgaagatga agtcaaagct atggggatag atacaacaga ccctaaagta gtaaatagcg
     1021 atgttgattt tgaaatagga gacacagtaa aagtatctca aggaccattt agtggacaaa
     1081 taggtaacat agaagaaatt gacttagaaa atagagaagt caaagtatgc ataaatgcat
     1141 ttggtaaaag gactctattt gtaatagagc ttgaaggtat agaaaaaatc taatactata
     1201 tagaaaaatc tgaggaggtg cgctcaaaat ggctaaaaaa gttataggtc aaataaaatt
     1261 acaaatacct gcaggaaagg ctactccagc gccaccagtt ggaccagcat taggacaaca
     1321 tggtgttaat ataatgggat tcacaaaaga atttaatgct aaaactgcag atcaagctgg
     1381 aatgataata ccagttgtta taactgtata tcaagataga tcattcagtt ttataacaaa
     1441 aactccacca gctgcagttt taattaaaaa agcattaaac ttaaaatcag gttctggaga
     1501 accaaacaaa aagaaagttg ctaaaatgac ttcagctcaa gtaagagaaa tagctgagtt
     1561 aaaaatgcct gacttaaatg ctgcatcagt agaagctgct atgagtatga tagctggtac
     1621 tgctagaagc atgggtgttg taatcgaaga ctaattaaaa gatacacttt ttttataaag
     1681 tgggaggcga gaagccgaaa ttaaccacaa ggaggaaaat aaaatggcta aaaaaggtaa
     1741 aagatatgca ggtgcattac aaaaagtaga tagaactaaa ttttatgatg catcagaagc
     1801 attaacatta gtttcagata tagctggagc taaatttgat gagacagtag aagcacacat
     1861 taaattaggt gttgactcaa gacatgctga ccaacaagta agaggtgcag ttgtattacc
     1921 tcatggaact ggtaaaacaa aaagagtttt agtttttgct aaaggtgaaa aagctaaaga
     1981 agctgaacaa gctggtgctg actttgtagg agctgaagaa ttagttcaaa aaatacaagg
     2041 tgaaaactgg tttgactttg atatagtagt tgctactcca gatatgatgg gtgtagtagg
     2101 tagattaggt agagtattag gacctaaagg tttaatgcca aaccctaaat caggaacagt
     2161 tacatttgat gtagctaaag ctatagatga aataaaagct ggtaaagttg aatacagatt
     2221 agacaaaact aacataatac acgttccagt aggaaaagta tcatttggtg gagaaaaatt
     2281 aactgaaaac tttactgcat taatggatgc tataataaaa gctaaaccag ctgctgctaa
     2341 aggacaatat ttaagaagta taacagtagc ttcttcaatg ggacctggag ttaaaataaa
     2401 cccagcaaga acagctgaat aataaaactg ttgacacagg ttgacaaaaa tgttattata
     2461 tcacttgtca atgtaaaaaa gaatagattt gccgtagata gtgggtgcga aagcttaata
     2521 tcccgccgag gttttagata aattgagatt actaaagctt ttgatgtcta cttttggcaa
     2581 caaaagcttt ttataatgtt gcggtaaata ttaagcctat ttggtgaagg aggtgcacat
     2641 ctatatgaga aaagctatag aaattaaatc agaagttgtt tctgagatag ttgaaaagtt
     2701 acaaaaatct tctgctgctg ttgttgtaga ttacaaagga ttaacagttg aagaagtaac
     2761 tgagttaaga aaacaaatga gagaagctgg tgtagattac aaagtataca aaaatacttt
     2821 agttagaaga gcagctaaag aagttggtat agagcaattt aatgatgaat tattagttgg
     2881 aactaatgcc atagcatttg gatatgatga tccagttgct ccagctagaa tattaaaagg
     2941 attcatggat tctcatccaa aaatgaaatt aaagatgggt attgtggaag gtgcattcta
     3001 tgatgaatct aagattgttg aaatggctaa cataccatca agagaagttc ttatcgctaa
     3061 attacttggt agcttaaaag ctccagtatc aaacttcgca tacttaatag atgcaatagc
     3121 gaaaaaggca gaaggtcaag aagaagctta attgcttaac taaaaaatta taaatattaa
     3181 tttgaaataa aattcggagg tgctaaaaat gacaatagaa caaatattag aagctataga
     3241 aaatatgaaa gttttagaat taaatgaatt agttaaagct gctgaagaaa aattcggtgt
     3301 atcagcttca gctccagtaa tggtagctgg tgcagctgct ggtggaccag ctgctgaaga
     3361 aaaaactgag tttgatgtag tattaactga cgttggttct tcaaaagttg gagttataaa
     3421 agcagttaga gaaataacag gattaggatt aaaagaagct aaagaagtag ttgacaatgc
     3481 tcctaagaca gttaaagaag gagcttctaa agaagaagct gatcaaataa aagagaaatt
     3541 agaagctgct ggagctaaag tagaagttaa gtagtttctt taaaaagata cccattgggt
     3601 atcttttttt atttataaaa aactataaag aaagtatttg gaattataaa gaaagtgttt
     3661 ttataaaata aaaaaaattt atataatagt actaaattaa gggtatcata tatggtgtaa
     3721 tggtatttta atttaacatt attatcaaat taaaatataa acaattgttg taatgtctgt
     3781 atataaagta tataattaaa ctaaaatgta aaattttaca ttttagttta aaaaaataca
     3841 cgagtgtttt ataaataaaa tgtaataaat aattaattaa caaatataaa ttttaaaata
     3901 agttagatat ttagaaaaat cgaaatttgt aggatttaaa ggacatattc aaggaggagt
     3961 taataatgga aaaattacaa ggaaaaattg cagtagttac tgcagcaaca aaaggtattg
     4021 gattagcatc agcagagata ttagcaaaaa atggagcaac tgtgtactta gcagctcgtt
     4081 cagaagaatt agctaatgaa gttataaata aaataagtgc agaaggtggt tgtgctaagt
     4141 ttgtttactt taatgctcgt gaagaagaga cctttacctc aatgatagaa gaggtagtta
     4201 aaaaagaagg taagatagat atacttgtaa acaattttgg ttcaacaaac ccttctcttg
     4261 acaaagacct tgtaactggg gatacagata atttctttga cacagtaaat actaatttaa
     4321 aaagtgtgta tttaccatgt aaagcagcaa ttcctcatat gataaagaat ggaaaaggaa
     4381 gtatagtaaa tatatcaagt ataggttcag tattacctga tttatctaga atagcttact
     4441 gtgtatcaaa agcagcaatc aactcattaa ctcaaaacat agctacacaa tatgctaaag
     4501 acaatgttag atgtaatgct gtacttccag ggcttattgc aactaaagca gcattagaca
     4561 atatgtctcc agagttcata aaagaatttt taaagcatgt tcctttaaat cgtatagggg
     4621 aaccagatga tatagcaaaa gctgttctat tctatgctag tgatgactca tcatttataa
     4681 caggggattt acttgaagtt gcaggaggat ttggtttacc tactccacaa tttgcagata
     4741 atatattagg ataattaaat tactaatatg caatgaaagc ttatgcatta gttatagtag
     4801 aaatgatatt tttataaagg ccatagatat aatttatatt ggaaaggttt gaggttcaaa
     4861 attccaagtt aaatttattt atggtcttta ttttgtatat tttaataaat tgtatatatt
     4921 tgtataaatg taattattta caatcatttt aatatggaat tataattaaa taggggggat
     4981 ggtacttgta taaaattttt tatagaaaat tagacctata aatgaaaaat atggatttta
     5041 tattggaaaa ttgtacataa tacataatat atgcttaaaa taatttacaa aaaatcaaaa
     5101 ataatgcata gtttacatat aagataaaaa atatattgaa aaatacttga cattatgcta
     5161 aaattattag atgcgttaga atgaactatt gttttatttt taattattga gaggtgaaaa
     5221 gtgaatgcca catcctgtca cgataggtaa aagaactaga atgagctttt ctaaaattaa
     5281 ggaaatagct gatgtgccaa atcttataga aattcaagta gattcctatg agtggttttt
     5341 aaaagaaggt ttaaaagaag tgtttgatga tatttcacca atagaagatt atactggtaa
     5401 tcttatattg gaatttgtcg actattctct agatgataaa ccaaagtacg atatagaaga
     5461 gtgtaaagag agagatgcta catattgtgc acctctaaag gttaaggtaa gacttataaa
     5521 taaagaaaca ggtgagataa aagaacaaga agtgtttatg ggtgacttcc ctttaatgac
     5581 agaaagagga actttcgtta taaatggagc agaaagagtt atagtaagtc aattagttag
     5641 atctcctggt gtttactatg cggaagaaag agataaaaca ggtaagagat tgatttcatc
     5701 tactgtaatt cctaatagag gagcatggtt agaatatgaa acagattcta atgatgtaat
     5761 atctgtaaga gttgatagaa caagaaaaca accagttaca gttttactta gagctttagg
     5821 aataggaaca gatgcagaga taattgacct tttaggagaa gatgagagat tatctgctac
     5881 attagaaaaa gataatacta agacagtaga agaaggtctt gtagagatat acaaaaaatt
     5941 aagaccaggt gaacctccta ctgtagaaag tgcatcatct ttattaaatg ctttattctt
     6001 tgacccaaaa agatatgacc tagcaaaagt tggtagatat aaattcaata agaaacttgc
     6061 tttatgttat agaataatga ataaaatttc agcagaagac ataataaatc cagaaactgg
     6121 agaagtattt gtaaaagctg gagaaaaaat aagctatgat ttggctaaag ccatacaaaa
     6181 tgcaggtata aatgtagtaa atctactaat ggatgatgat aaaaaagtta gagttatagg
     6241 aaataatttt gtggatatta agtctcacat tgattttgac atagatgatt taaacataaa
     6301 agaaaaagta cattatccaa ctttaaaaga aattttagat ggatatagtg atgaagaaga
     6361 aataaaagag gctataaaat caagaattaa agaacttata ccaaaacata tattattaga
     6421 tgatataata gcttcaataa gttatgagtt caatatattc tataatatag gaaatattga
     6481 tgatatagat cacctaggaa acagaagaat aagatctgta ggtgaattgt tacaaaatca
     6541 agttagaatt ggtctttcaa gaatggaaag agttataaaa gaaagaatga cggttcaaga
     6601 tatggaagct ataacgcctc aagcgttagt taacataaga ccagtttcag ctgcaataaa
     6661 agaatttttt ggtagttctc agttatctca gttcatggat caaacaaatc ctctatctga
     6721 gttaacgcat aagagaagat tatcagccct tgggcctgga ggtctttcaa gagaaagagc
     6781 tggattcgaa gtgcgtgacg ttcaccattc acactatggt agaatgtgtc caatagagac
     6841 tccagaggga ccaaatatcg gtcttataaa ctctctagga acttatgcaa aaataaatga
     6901 atttggattt atagaatcac catatagaaa atttgataaa gaaacatcaa cagttactga
     6961 tgaaatacat tatttaactg ctgacgaaga agatttattc gtaagagctc aggcaaatga
     7021 gccattaaca gaagatggta aattcgtaaa ccatagagtt gtttgtagaa ctgtaaatgg
     7081 tgctgttgaa atggttcctg aaagtagagt agattacatg gatatatctc ctaagcaggt
     7141 tgtatctgtt gccactgcta tgataccttt cttagaaaat gatgatgcca accgtgccct
     7201 tatgggagcg aacatgcagc gtcaggcagt gcctctagtt agaagagaag caccaattat
     7261 aggaactggt atagaataca gagcagcaaa agactctgga gcagttgttg ttgctagaaa
     7321 ttctggtata gcagaaagag taacagcaga tgaaataatt ataaaaagag aagatggaaa
     7381 cagagataga tataatctac ttaagtttaa acgttcaaac tcaggtactt gtataaatca
     7441 aacacctata ataaataagg gtgaccagat aataaaaggt gatgttatag cagatggtcc
     7501 agcaactgat ttaggagaag ttgcacttgg aagaaactgt cttatagcat tcatgacttg
     7561 ggaaggttat aactacgagg atgctatatt aataaatgaa agattagtta aagaagatag
     7621 attatcaaca attcatatag aagaatatga atgtgaagct agagatacaa aactagggcc
     7681 agaggaaata actagagata tacctaatgt tggagatagt gcaatcaaaa acttagatga
     7741 tagaggtata ataagaatag gtgcagaagt agactcaggt gatatattag ttggtaaggt
     7801 aactccaaaa ggagaaactg aacttactgc tgaggaaaga ttacttcgtg cgatattcgg
     7861 agaaaaagcc agggaagtta gagatacttc acttaaagta cctcatggtg aatctggtat
     7921 aatagttgat gtaaaagtat ttactagaga aaatggagat gatttatctc caggagtaaa
     7981 tgaattagta agatgttata tagctaaaaa gagaaagata aaagttggag ataagatggc
     8041 tggtcgtcat ggtaataaag gggttatctc gagagtatta cctgaagaag atatgccatt
     8101 catggaaaat ggaacgccat tagatataat acttaaccca caaggtatac catcacgtat
     8161 gaacatcggt caggttctag aagttcattt aggacttgct gctaagacat taggatggta
     8221 tgtagcaaca tcagtattcg atggtgctaa tgagtatgac ataatggatg ctcttgaaga
     8281 agctggatat cctagagatg gtaaattaac tttatatgat ggtagaacag gtcaatcttt
     8341 tgacaataga ataactgttg gatatatgta ttacttgaaa ctacatcact tagtagatga
     8401 aaaactacat gcaagaagta ctggaccata ctcattagtt actcaacagc cactaggtgg
     8461 taaagcacaa tttggtggac aaagatttgg agagatggag gtttgggctc tagaagcata
     8521 tggagcggct cacatacttc aagagatact tactgttaag tctgatgatg ttgtaggtcg
     8581 tgttagaact tatgaagcta tagttaaagg tgaaaacata cctgagccag gaatcccaga
     8641 atcatttaag gttcttataa aggaacttca aagtttatgc ttagatgtaa aagtattgac
     8701 agatgaagac caagaaatag aagtaagaga atctgtagat gaagatgata cgataggtga
     8761 atttgagcta gacgttgtaa atcacatggg agaagttgaa gaaagtaata taatagaaga
     8821 aattgaagat gattttgtgg aagccacaga agatgaggat atagagaatt tagaagaatt
     8881 cactgaagat gatttatttg aagaagaaat agattttgaa agtgatgact ttgatatgta
     8941 aatttaatta atgtaaatct caaatagaag ggagagaact ccttgtttga attaaacaat
     9001 ttcgagtcga taaaaatagc attggcttct ccagaaaaaa taagacaatg gtctagagga
     9061 gaagttaaaa agccagaaac tataaattac cgtactttaa agccagaaaa agatggtctt
     9121 ttctgtgaaa ggatatttgg accacaaaaa gactgggagt gtcattgtgg taaatataga
     9181 agagttagat ataaaggtgt agtttgtgat agatgtggag tagaagtaac taaatcaaaa
     9241 gtaagaagag agagaatggg acatatagag ctagctgctc ctatgtctca catctggtac
     9301 ttcaaaggta taccaagtag aatgggactt ttacttgata tgtcaccaag atcgttagaa
     9361 aaaatactat atttcgcctc atatgtagta gttgatccag gtgaaactgg attgaatgaa
     9421 aaacagttac ttacagaaaa agaatacaga actgctcttg aaaagtatgg atatactttt
     9481 actgtgggta tgggtgctga agctgtaaaa acattactac aaaatataga tttagaacaa
     9541 caaagtaaag acttaagagc ggaattaaaa gatagtacag gacaaaagaa agtaagaaca
     9601 attagaagat tagaagttgt agaagcattt aaaaaatctg gaaataaacc agaatggatg
     9661 attttagatg caataccagt aataccgcca gatttaagac caatggtaca acttgatggt
     9721 ggaagatttg caacttcaga cctaaatgat ttatacagaa gagttataaa tagaaataat
     9781 agacttaaaa gactattaga gcttggagct ccagatataa ttgtaagaaa tgaaaaaaga
     9841 atgcttcaag aagctgttga tgcattgata gataatggta gaagaggtag acctgtaaca
     9901 ggacctggaa atagaccact taaatcttta tcagatatgt taaaaggtaa gcaaggtcgt
     9961 ttccgtcaaa acttacttgg taagcgtgtt gactactcag gacgttctgt tatagttgtt
    10021 ggaccagaac ttaaatttta tcaatgtgga cttccaaaga agatggcatt agaattattc
    10081 aagccatttg ttatggataa attagttaaa gaaggttatg cacataatat aaaaagtgcg
    10141 aaatctatag tagaaaaagt taagccagaa gtttgggatg ttttagaaga tgttataaaa
    10201 agtcatccag ttcttcttaa ccgtgcaccg actctacata gattaggtat acaagcattt
    10261 gaaccaatct tagttgaagg taaggctata aaactacatc ctcttgtatg tacagcttat
    10321 aatgcagact ttgatggtga ccaaatggca gtccatgtac ctttatcagt agaagcacaa
    10381 gcagaagcaa gattcttaat gctttctgta aataatatac ttgctcctaa agatggttca
    10441 cctataacta ctccatctca ggatatggtt ttaggttgct attatctaac aatagaagcc
    10501 caagatggag ctaaaggaac aggtatggta tttaaagact ttaatgaact attacttgct
    10561 tattacaata aatcagttca tctacatgca ttagtgaaac tgaaggtaac acttgaagat
    10621 ggaagaagtt cattggttga aagtactgtt ggtagattta tatttaatga aaatatacct
    10681 caagacttag gttttgtaga tagaaaagaa aatccatttg cacttgaagt tgatttctta
    10741 gcagataaaa aatctcttgg taaaataata gataaatgct ttagaaaaca cggaaataca
    10801 gaaacagctg aattactaga ttatataaaa gctctaggat ttaaatattc tacattaggt
    10861 ggtataacag ttgctgttga tgatatgagt gtcccagaag aaaagaaagt atttatagct
    10921 gaggcagaag ctaaagttga taagtatgaa aaagcataca gaagaggtct aatctctgat
    10981 gaagaaagat atgaaaaagt tatagagaca tggacagaaa caactgataa agttactgat
    11041 gctcttatgg gtggactaga tagattaaat aatatatata taatggcaca ttcaggagcc
    11101 agaggttcta aaaaccaaat tagacagctg gcaggtatgc gtggtcttat ggcaaatgca
    11161 tctggtaaaa cagtcgagat accagttaaa tctaatttcc gtgaaggttt atcagtacta
    11221 gaatacttta catcttcaca tggtgccaga aaaggtcttg ccgatacagc tatacgtaca
    11281 gctgaatctg gatatttaac aagaagactt gttgatgtaa gtcaagatgt tattgtaagg
    11341 gaaatagatt gtggaacaga agatactaca gagatttatg ctataaaaga aggaaatgaa
    11401 gttatagaag agatatatga tagaattgta ggaagatata ctatagaccc tatattaaat
    11461 cctgaaactg gagaagttct agttgaggct gattcaatga tacaagaaga tgaagcagaa
    11521 actatagtag ctttagggat tgaaaaaatt aggataagaa cagttcttaa ctgtaaaact
    11581 aatcatggag tttgttctaa gtgttatggt agaaacttgg caacaggaaa agaagttaat
    11641 ataggtgaag cagttggtat aatagcagct caatctatcg gtgagccggg tactcagctt
    11701 acaatgcgta cattccatac tggaggagtt gcaggagctg atataactca aggtctacca
    11761 agggttgaag aattatttga agctagaaaa ccaaaaggat tagctgtaat aactgaagta
    11821 tctggtagag ttgaaattga tgaaacagga aagagaaaag aagtaaatgt aataccagaa
    11881 gaaggcgaga ctcaaacata tgtaattcca tatggttcta gattaaaagt taagcaaggt
    11941 caaatgctag aggctggaga ccctctaaca caaggattta taaatcctca tgatatagta
    12001 agagtaaatg gtgttaaggg agttcaagaa tatatagtta aagaagttca aagagtgtat
    12061 agacttcaag gggttgatgt taatgataag catatagaag ttatagtaag acaaatgcta
    12121 tctaaagtaa aagttgaaga cccaggagat acagatttat tgccaggtgg atatgaagat
    12181 gtattaacat tcaatgaatg taataaagat gctatagata aaggtctaag accagcagtt
    12241 gctaaaagag ttttacttgg tataactaaa gcatctcttg caactgattc attcttatca
    12301 gcagcttctt tccaagaaac aacaagagta ttaacagagg cagcaataaa aggtaaagaa
    12361 gaccacttaa taggacttaa agaaaatgtt atattaggta agttaatacc agcaggaaca
    12421 ggaatgaaga aatatagaaa tatagctgtt gaaaaaattg aagattaata gcatatagag
    12481 taaagaaatc ttgacaacca ttatttgtgg tgttaaaata gtaaagtgtg ttgtttaaaa
    12541 gtacactaag ggcttgaaca agttcttagt gacttttaga tggcaataaa tgagaactaa
    12601 aaagcatcaa gctagaaaag tgctaatatc acatatttat gtgttataga cattaaattg
    12661 taagtaacga atgttacaat cagtcagtat gaatattcat attgatgtag ttggcggtca
    12721 ctgataagat cgaaaatttg gaaggaggtg cagatgatgc caacaattaa ccaattagtt
    12781 cgtaaaagta gaaaagcgct agagaagaaa tctactgctc cagcattaca aaaaggttac
    12841 aactctttaa ataagaaagt aactgacgct agtgccccac aaaaaagagg ggtttgtact
    12901 tcagtaaaaa cagttactcc taagaagcct aactcagctt taagaaaagt tgccagagtt
    12961 agattaacta atggtataga agtttctgct tatataccag gagaaggaca caacttacaa
    13021 gagcatagtg ttgttcttat aagaggagga agagtaaaag accttccagg ggttagatac
    13081 catatactaa gaggtactct agatacagca ggtgttgata agagaagaca atcaagatct
    13141 aaatacggtg cgaagagacc taaagaagct aaaaaataaa acaattaatg attgattata
    13201 gttcggtgct ttaaatatat atatatttac ggcatcacgg cattaaatga tttatgtcga
    13261 gtacctatga tttaagttaa ataattaagg agggaagcga aatgccaaga aaaggtaata
    13321 ttccaaaaag agaagtttta ccagatccta tgtatgggag taaggtagtt actaaattaa
    13381 taaataatct aatggtagac ggaaaaaaag gaaagtctca aagaatagtt tatgatgctt
    13441 tcgcaattgt tgctgaaaag acaggagaag aagctttaga agtatttaat aaagcaatgg
    13501 ataatataat gcctgtttta gaagtaaaag caagaagagt tggtggggca aattaccaag
    13561 tgccaatcga agttagacct gaaagaagac aaacattagg tttaagatgg ctagtaaaat
    13621 atactagagc tcgtggagaa aaaggaatgg ttgaaaagct agctaaagag ataatggacg
    13681 cagctaacaa cactggagct tcagttaaga aaaaagaaga tactcataaa atggcagaag
    13741 ctaataaagc atttgctcat tacagatggt aggatatata gatatcttat aaattcaagg
    13801 atatcataag gaggataacc atggctagaa agtttccttt ggaaaaaact aggaatatag
    13861 gaatcatggc tcatatagat gcaggaaaga ctactactac agaaagaatc ctgttttata
    13921 ctgggcaaac tcataaaata ggagaaactc atgaaggagc ttctcaaatg gactggatgg
    13981 agcaagaaaa ggaaagaggt ataacaataa cttctgccgc tactactgct tcatggaaag
    14041 accatagaat aaacataata gatactcctg gacacgtgga cttcacagtt gaggttgaaa
    14101 gatctctaag agttcttgat ggttcagttg ctgtattctg tgcaaaaggt ggggttgagc
    14161 ctcaatctga aaatgtatgg agacaagctg aaacttatgg agtacctaga atagctttta
    14221 taaacaaaat ggatatatta ggtgcagatt tctacaatgt tataagcatg atgaaatcta
    14281 gattaaattc taatgcagtt cctatgcaat taccaatagg taaagaagat tcatttatag
    14341 gtataataga cttattaaaa atggatgctg ttatatacaa agacgaccta ggggttgaaa
    14401 tggaagaaac agatatacca gaagatatga aagaattagc tgctgagtgg agagaaaaat
    14461 tagttgaatc agtagctgaa actgatgaag aattaatgat gaaatacctt gaaggtgaag
    14521 aacttactat agatgaatta aaagttgcta taagaaaagc tactatagct tgtgaaatga
    14581 acccagtttt ctgtggtaca gcttatagaa acaaaggtgt tcagttagta atagatgcag
    14641 ttttagatta cttaccagca ccaactgata taccagctat aaaaggtata ttagcagatg
    14701 gagaagaagc tgaaagacat tcttctgatg aagagccatt ctctgcatta gcattcaaaa
    14761 taatgactga cccattcgtt ggaaaactag cgttcttcag agtttattct ggaacattag
    14821 aatcaggttc ttatgtttta aatgcaacta agaataagag agaaagaatt ggtcgtatat
    14881 tacaaatgca tgctaataca agagaagaaa taactaaagt gtatgctgga gatatagcag
    14941 cagcagtagg attaaaagat actactactg gagacacatt atgtgaccct gctaatccaa
    15001 taatacttga atctatggaa ttccctgagc cagttatatc tgttgctata gaaccaagtt
    15061 ctaaagcagc tcaagaaaaa atgggtatag ctcttcaaaa attagctgaa gaagacccaa
    15121 ctttcacagt taagactgac caagaaacag gacaaactat aatatctggt atgggtgaat
    15181 tacacttaga gataatagta gatagattat taagagaatt taaagtagaa gctaaagttg
    15241 gtgcaccaca agttgcatat agagagacta taactcaacc agttgatgtt gaatacaagt
    15301 actctaagca atcaggtggt agaggacaat acggtcacgt taagattaga gttgctcctc
    15361 aagagccagg tgaaggatac aagttcacaa acaaaactgt tggtggatca gtaccaaaag
    15421 aatatgttgg tccagttgac atgggtatac aaggagctat gcaatctggt atagttgctg
    15481 gatatccagt tgtagacgtt gctgttgagt tatatgatgg ttcttaccat gaggttgact
    15541 cttctgagat ggcgtttaag atggctggtt caatggcatt caaagatgct atgaagaaag
    15601 gtaatgcagt tcttttagag ccttacttca aagtagaagt tgttactcca gaagattaca
    15661 tgggagatgt tatgggtgac ttaaactcaa gaagaggatt aatccaaggt atggaagcta
    15721 gaagtggtgc acaagttata aatgcattcg ttccactttc agaaatgttt ggatactcaa
    15781 cagacctaag atcttcaact caaggtcgtg caacttacac aatgatattc gatcactatg
    15841 agcaagttcc agcttcagtt gctaagaaaa tagctgaagg aaaataattt aaaaataatt
    15901 aataatataa atataaaaaa taatcagcta gatgagatat atctcatcta gcacaagat
//
LOCUS       DAMJWP010000072        15725 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.076, whole genome shotgun sequence.
ACCESSION   DAMJWP010000072 DAMJWP010000000
VERSION     DAMJWP010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15725
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.076"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(108..968)
                     /locus_tag="PC700_001883"
     CDS             complement(108..968)
                     /locus_tag="PC700_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HDF2581194.1"
                     /translation="MAMKWRDYADDVNLFEDFDKTIKPLTDEQRRKNINTLIAYFSKE
                     VPSKIYNLSNDEIKPRDILRGLLNVYPPKEIAPEILNMLHNLLLIECEERELVDVNDI
                     EEVEEGIAIWRGNITNLRADAIVNAANNKLLGCLQPLHLCVDNEIHSCAGPRLREDCD
                     KIIKKQGHLEYTGDAKITRGYCLPAKFVVHTVGPIVSGGQPSKEQEKQLLHCYKSCLN
                     TIKEIDEIKNIVFCGISTGVFGYPKKEAANLAVSRVRLWLKENPEKNLKVVFNVFTEE
                     EEEKYRRIFK"
     gene            complement(1285..2613)
                     /locus_tag="PC700_001884"
     CDS             complement(1285..2613)
                     /locus_tag="PC700_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDF2581195.1"
                     /translation="MEKFSVVIAGGGSTYTPEIILMLLDNLDRLPLRAIKLYDNDEER
                     QNKVAKACEILIKEKDPNIEYLATTCPKEAYTDVDFCLAHIRVGKLEMRELDEKIPLK
                     HGVVGQETCGPGGIAYGMRSIEGVLENIDYMEKYSPNCWMLNYSNPASIIAEAVRRLR
                     PNSRVINICDMPIGMEHNIARIAGLKSRKHMDIRYFGLNHFGWYTSIKDKKGNELLPK
                     LVEHIKEYGFINGEEGMKNDKKDSWFETNLFTKEIVKTDLTTIPSSYLKYYLFPDYVV
                     NHSDVNYTRANEVIDGREKEVFGSCALIEKQGHSKGSGLKIGIHAEFIVDLATALAFN
                     TQERMLLIVQNNGAIENIQDDAMVEIPCIVGKDGYEPLSIGKIPTFQKGLIEQQLAVE
                     KLVVDAWIEKSYNKLWQALMLSKTVPSATVAKELLDDLIEANKNYWPKLN"
     gene            complement(2761..3090)
                     /locus_tag="PC700_001885"
     CDS             complement(2761..3090)
                     /locus_tag="PC700_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2581196.1"
                     /translation="MVPEMEEKVFSIISEAGDAKSDVMMSLKEIKKGDYNKAKHLLNS
                     ASEKIQTASKHHLELLSNAMNSEDSGTDFLVVHSEDHYSNALFAHSLVSELVDIFEMM
                     DPRINKN"
     gene            complement(3114..4403)
                     /locus_tag="PC700_001886"
     CDS             complement(3114..4403)
                     /locus_tag="PC700_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2581197.1"
                     /translation="MEALQNNLRRFLLPIAQKIEKQRHLQAIKEGMISITPIIIVGSL
                     SMLFMALNNMLPEGSAKTLLSENMDTLLIPNKFTMSLLSIYSAFFIAQALAKKYNLNH
                     VEIGMTAVVAQLVVCGQVVDGVLDTSYLDAQGLFVSILVALLVVDITKFMNDKNLVIR
                     FPKEVPSVVNKSFRNLTPMIVCIMLFTAIAAITKNVSGQPLPAIIMNFLAPAISSVDN
                     VFAVTIILFITQLLWFFGLHGAAITSSIWMPIAATYMAENATLIAAGGDPKYVFTIGF
                     YYGFLQVTGSGITLGLVYLMSRSKCKSFNSMGKVVILPSLFGINEPVIFGTPIVMNPY
                     MFVPFVFGPVLVGALNFMALKVGLVGLPIAEPPGFLPPGVAAFLMTLDWKAIVLVFAS
                     IILMTLIYYPFFKIMEKEELNKNAELATTLDDDSFDF"
     gene            complement(4419..4745)
                     /locus_tag="PC700_001887"
     CDS             complement(4419..4745)
                     /locus_tag="PC700_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2581198.1"
                     /translation="MNILLVCANGASTGVLVEKMKSFCSEHEKLKTKTINIEATSFEN
                     LKSYIEANDTDVVLVAPQIRFKEDDVVEACKNYKIAVGLIDTKHYGRMDAPSVMKSAI
                     ELYKNR"
     gene            complement(4880..5509)
                     /locus_tag="PC700_001888"
     CDS             complement(4880..5509)
                     /locus_tag="PC700_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581199.1"
                     /translation="MQDKILRDKKANDPWARMTTRNGFSADEIISSFQKAIRRNMVEE
                     ACEFAYELYISSPELEDKLWRRILTISVEDIGMGDPSAVIIINNLNQVRKEYSYADGD
                     RPLFFIHAIRYLCACEKDRSSDLLKNIIIKGFAMGKVPEVMDVALDKHTYRGLEMGRD
                     SFHFLNEASIVIPEKEVDNDYKERYLKILEKYKQEEVIDTAFKFNHWQY"
     gene            complement(5724..6740)
                     /locus_tag="PC700_001889"
     CDS             complement(5724..6740)
                     /locus_tag="PC700_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2581200.1"
                     /translation="MVKAVGDIIGKCSAISFDMKLDKSYDEVIVDFKNLINNIDNKDV
                     LLFTDMGSLNSFDEIIKKEKKCEVRVIPMVMTLTVLEAVQKANMGLPLNDVYNSITNT
                     RKYYFGTNEIQNKENLSKTIIIASHVSEGVDNKTRKILEEKMSRYLDGIDIISVPYKT
                     EKDLSLNITKLKESSNIVAVINEQRINIRGIDYISKKDIDKDENINKLKNIIKISIGY
                     DDVVEGLKTSLKSSNYNRIFKDIKYVSDELFLVFNIEKKYDKVIGLMMHLAFMVDGLI
                     GNTREIEKLDKEKTLDYHKSLSKIKDIVSQLDKKYNIEINEKECYQILLILEYAEIIE
                     KDYQ"
     gene            complement(6721..8379)
                     /locus_tag="PC700_001890"
     CDS             complement(6721..8379)
                     /locus_tag="PC700_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2581201.1"
                     /translation="MIYKKDKVMKVLESSYRENEKGITANQLSEKTGIARNNISTYLN
                     QLFNEGMVTKIKGRPVYYIPANLNLNKNELLDTQSELLGIKKGTKVENKIKKDSFETL
                     IGKEHSLRPIIEKCKVAMLYPTNGLHTIIYGETGVGKSMIARYMYDYSIDSGIRKNKA
                     PFVTFNCADYANNAQLLMGHIFGVEQGAYTGAESSRKGLLEIANGGILFLDEIHRLPA
                     EGQEMLFTFIDKGTFKRLGDATKERDSKVLIICATTENPTSTLLDTFNRRIPMKIEIP
                     SLRDRTMIERMELVKNFFKEESKSISISIKVHKEIIKSLLLYDCKNNVGQLQNDIKLA
                     VANGYLRYKRDNKEPVYIEKKYFDKSINEVSDDYREKSIKADEVITHGIEYFVFTSLG
                     EEEVINYNDINIINAVNTNLNKSVEMNIADASLFNNVKFKSMCESIRQIIKEDEEIDI
                     SESIFKSMAIYIKSVLDKSSSTNNIDLNQIRRNNRSEFKTALKIVGIIENEFDLFLSI
                     ETVAYITLFIVQAKQDEQNKIVNNINIIVAMHGETTASNGKSSW"
     gene            complement(8738..9682)
                     /locus_tag="PC700_001891"
     CDS             complement(8738..9682)
                     /locus_tag="PC700_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009527644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2581202.1"
                     /translation="MNREKILEIKNLKQYFHLDKSTTVKAVDDISFDIYKGEIFGLVG
                     ESGSGKSTTGKTIIRLHESTGGEVIYKGNCISDKKTYKFIKKDVNKSMQIIFQDSTSS
                     LNPRMTIADIISEPLKIQGICKNKTDRMNKVYEMLKLVGLDRSYANKYPSDFSGGQRQ
                     RIGIARALSVDPEFIIADEPIASLDVSIQAQIVNLFKKLQQEKNLTCLFIAHDLSMVR
                     HISDRIGVMYNGKLVELADSNELYNNPIHPYTKSLLSAIPVPDPRYAKSRNRIEYNSN
                     GYDCSNEKSLSWIEVSDGHFVYSSKSEINKYQQNLKVV"
     gene            complement(9675..10700)
                     /locus_tag="PC700_001892"
     CDS             complement(9675..10700)
                     /locus_tag="PC700_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2581203.1"
                     /translation="MEHLLEVNNLSVSFKVEEGEVQAVRNVSFNLKKGETLAIVGESG
                     CGKSVLCKSLMRILPYNGYIKNGEVLLKSSDLVKKSEKEMEDIRGKNISMIFQDPMTS
                     LNPTISIGKQIAEAVIIHQGISKSEAKKRAIELIELVGIDNPEKRFKQFPHHFSGGMR
                     QRIVIAIALACNPDVLIADEPTTALDVTIQAQIIDLIKDLQHKIGLSIIFITHDLGVV
                     ATIADRIAVMYAGKIVEIGTVEDIFYDPRHPYTWGLLGSLPTLDSQDEYLYNIPGMPP
                     NLLNPPKGDAFAIRNKNALKIDYEKEPPMFKINDTHSAATWLLHPDAPEVDVPVRVNC
                     GRVISNE"
     gene            complement(10775..12343)
                     /locus_tag="PC700_001893"
     CDS             complement(10775..12343)
                     /locus_tag="PC700_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2581204.1"
                     /translation="MKLKKLKVLSLVMILSLMAGCSSGGDKDKKADTPKDGKVLVYGS
                     NDYTSINPALYEHGEINSLIFNGLTAHDENNKVVPCLAKDWKFDEATNTYTFNLRDDV
                     KWHDGEKFTANDVKFTIETIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAM
                     LDYLTVGVLPKHVLEGKDIATDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEP
                     GLDKVVFKIVPDDKAKAMQLKSGELDLAQITPKDMSNFEKDEKNFKVNIMKTADYRGI
                     LYNFNSKFFKDKKAKGLPNALSYAIDRKAIVDSVLLGHGVPAYSPLQMGPYNNPDIEK
                     FEYNPEKAKQEIEKLGWKLGSDGIYEKEGTKLAFEITAGESDQVRVDMAKICAQQLKE
                     IGVDAKAVVVTETDWANQDAHLIGWGSPFDPDDHTYKVFGTDKGANYSAYSNPTIDKI
                     LQKARETEDKDEKLKLYKQFQVEMTKDMPYTFIAYIDAIYVGKPNIKGLTPDTVLGHH
                     GVGIFWNIADWTIE"
     gene            complement(12456..13367)
                     /locus_tag="PC700_001894"
     CDS             complement(12456..13367)
                     /locus_tag="PC700_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2581205.1"
                     /translation="MKPQYSDFEIVGENYILVAEDEHIETKKTLSKKIKELPYISIII
                     LSIIVIGSVFSSFIMTHEPTYMDLASSNLAPNKTFLFGTDSMGRDIYSMIWYGGKISL
                     FIGLFSTVISTVIGIVYGSISGSASELVDDAMMRFTEIILSIPSILIIIFVQAILGNS
                     NPVSMSIVIGITSWMNISKIVRTEVRQIRNSEYILAAKSMGGGFFYVLKQHLLPNFVA
                     SIMFMVVTNIGAAIGTESTLSFLGIGLPIEIVSWGSMLSLSEEALLSNRWWIILIPGI
                     FLVTTLVCITNIGNYIRKNNNKKSSNL"
     gene            complement(13351..14337)
                     /locus_tag="PC700_001895"
     CDS             complement(13351..14337)
                     /locus_tag="PC700_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2581206.1"
                     /translation="MLKDILKKLLQFILVMFLLSFIVFYMARLAPGDPLISYYGDGVE
                     RMSTQEKENAMKKLGLNEPIYSQYIKWISNASKGEFGISFKYKQNVTSVINDVYINTI
                     ILGGSGYILTFVLALLLGIFCTLHEDKLIDRIICKLGTITNCIPSFWVALVLILIFSI
                     NLSILPSSGAYSMGEESSILSRISHLILPLTVLILSHLWYYTYMIRNKLLEEIREDYV
                     LLCKAKGLNNRTIVFKHCLRNIMPSYISIMAISIPHILGGTYVVEKVFSYPGLGTLCF
                     ESAKYHDYNMLLVLCLITGALVVFGNMLAQIINNKIDPRMKYDRGDTNEATI"
     gene            14696..15097
                     /locus_tag="PC700_001896"
     CDS             14696..15097
                     /locus_tag="PC700_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2581207.1"
                     /translation="MLLKELYSKTMYTVEENFKENGLTHQQIIVIKLVAHNQELTISQ
                     LCDEMSLAKGTVSGIISRLEQIGYIEKFKKSNDKRNTYVKFTTTGFEFATNFKIKMQE
                     SFDDIFKNCDENELSDLVKNLRNILAKVKER"
     gene            15100..15498
                     /locus_tag="PC700_001897"
     CDS             15100..15498
                     /locus_tag="PC700_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03987 family protein"
                     /protein_id="HDF2581208.1"
                     /translation="MNYKLILAIVFITSALIFYTIGVFGERKAKILKKKHVIIFWLGF
                     IFDTLGTFTMSNIANSHTFEVKSALSQNLHSITGLLAIVLMLFHASWATFVLYKDDEE
                     KKKFFHKFSIVVWTIWLVPYFIGMFIGMVG"
BASE COUNT         4956 a         2730 c         1737 g         6302 t
ORIGIN      
        1 taaaaataca aattatattt tattaatttt tactacttgt aaattattat cttatacaat
       61 gaatttgtat tatataataa gtatttttag gattctgtta cataatatta tttaaatatt
      121 cttctatatt tctcttcttc ttcttcagta aacacattaa atacaacttt taaatttttt
      181 tcaggatttt cttttagcca tagtctaact ctacttacag caagattagc tgcctctttt
      241 tttggataac caaatacacc tgtagaaatt ccacaaaaaa caatattttt tatttcatca
      301 atttctttta ttgtatttaa acatgattta taacaatgaa gcagttgctt ttcttgctct
      361 ttacttggct gaccaccaga tacaataggt ccaacagtat gtactacaaa ttttgcaggt
      421 aaacaatagc ctctagttat cttagcatct ccagtatatt ctaaatgccc ttgttttttt
      481 attattttgt cacaatcttc tctaagtcta ggacctgcgc atgaatgtat ctcattatcc
      541 acgcatagat gtaatggttg taaacaccct aataacttgt tatttgcagc atttactatt
      601 gcatctgctc ttaaattcgt tatattacct ctccatatgg caatcccttc ctctacttct
      661 tctatatcat ttacatctac taactctctt tcttcacatt caatcaacaa taaattatgt
      721 aacatattaa gtatctctgg agcaatttct tttggtggat atacattcaa aagtcctcta
      781 agtatatctc taggcttaat ttcatcattt gataaattgt atattttaga aggaacttct
      841 tttgaaaaat atgcaattaa agtgtttata ttttttcttc tttgttcgtc agttagaggc
      901 ttgatagttt tatcaaaatc ctcaaataaa tttacatcat cagcataatc tctccacttc
      961 atagccatct ctccattctt gtattttaga cttaaaaata attatgtatt tatgtacata
     1021 tttatttatt tctataaaca cctacataaa tcctgttaaa agtgtaaatt ttttattatg
     1081 caacttctct ggttatttct attgattgat agtaaattat tgtacctaaa tttaatccaa
     1141 ctaacaagta actaaaccta ctttcctaac tgcaaaatac actatgtttg ccacttgtat
     1201 ctcatttgta aataaataaa gtccctacaa atacattaac taggctatgt actaaactgt
     1261 aggaacttta aaaatatatt attcttaatt aagttttggc caataatttt tatttgcttc
     1321 tattaaatca tctagtaatt cttttgcaac tgttgcacta ggaactgtct tagatagcat
     1381 aagtgcttgc cacaatttat tataagactt ctcaatccaa gcatcaacta ctaatttttc
     1441 aactgctaat tgttgttcta ttaaaccttt ttggaatgtt ggtattttcc ctattgataa
     1501 tggttcatac ccgtcttttc caactataca tggaatttca accatagcat catcttgtat
     1561 attttctata gctccattat tttgaactat caataacata cgctcctgtg tattaaaagc
     1621 tagagctgta gctaaatcca ctataaactc tgcatgtatt cctatcttaa gcccactgcc
     1681 cttagaatgt ccttgctttt ctatcaatgc acatgaacca aaaacttctt tttctcttcc
     1741 atcaataact tcattggcac gagtataatt tacatcagaa tgatttacaa cataatctgg
     1801 aaatagatag tactttaagt aactacttgg tattgtagtt aaatctgttt ttactatttc
     1861 tttagtaaat aaattagttt caaaccaact atctttcttg tcatttttca ttccttcttc
     1921 accatttata aaaccatatt cttttatatg ttctactaat tttggtaaaa gttcatttcc
     1981 ttttttatct ttaattgaag tataccaacc aaagtgattt aatccaaagt atcttatatc
     2041 catatgttta cgagatttga gtcctgcaat ccttgctata ttatgttcca tacctattgg
     2101 catatcacaa atatttatta ccctagaatt aggtctcaac cttctaacag cttccgctat
     2161 tattgatgct ggattagaat aatttaacat ccaacaattt ggagaatact tttccatata
     2221 atctatgttt tcaagaacac cttctataga cctcattcca taagctattc cacctggtcc
     2281 acaagtttct tgaccaacta ctccatgttt tagaggtatc ttttcatcta gttctctcat
     2341 ttcaagtttt ccaactctta tatgagctaa acaaaaatct acatctgtat aagcctcttt
     2401 agggcatgtt gttgctaaat actctatatt agggtccttt tcctttatta gtatttcaca
     2461 agctttagct accttatttt gtctttcttc atcattatca tataatttga ttgctcttaa
     2521 tggtaaccta tctaagttat ctagcaacat taatatgatt tctggggtat atgtacttcc
     2581 tcctccagct attactactg aaaatttttc cataataata tctccttttc tatttaaaaa
     2641 tttactgtat ttataaataa atttataaat actagtactt taaagtaaaa gggagttcat
     2701 ttattaaatc tattgattta ataaactata gccaaacttc aagcactatt tacaagaaat
     2761 ttaattctta ttaattcttg ggtccatcat ctcaaatata tctacaagtt cagatactaa
     2821 agaatgagca aataatgcat ttgaataatg gtcttcagag tgcacaacta aaaaatcagt
     2881 cccactatct tcactattca tagcatttga tagtaattct aaatgatgct ttgatgctgt
     2941 ttgtattttc tctgaggctg aatttaaaag gtgttttgct ttattgtagt cacctttttt
     3001 tatttcctta agagacatca ttacatcact tttagcatct cctgcttctg atattatact
     3061 aaatactttt tcttccattt caggtaccat atccttctcc ttctaatgac ttgttaaaaa
     3121 tcaaagctat catcatctaa tgttgtagct aattctgcat ttttatttaa ttcttccttc
     3181 tccattatct taaagaatgg ataatatatc aaggtcataa gtattatact tgcaaatact
     3241 aatacgattg ccttccaatc taatgtcatc aaaaatgctg caacccctgg aggtaaaaag
     3301 cctggtggtt ctgcaatagg taatcctact aatcctactt ttaatgccat aaaattaagt
     3361 gctccaacca atacaggtcc aaatacaaat ggtacaaaca tatatgggtt cataactatt
     3421 ggtgttccaa atattactgg ctcatttatt ccaaatagtg atggtaaaat cacaactttt
     3481 cccatactat taaaactctt acacttactt cttgacataa ggtacactaa tcccaaagtt
     3541 atccctgaac cagttacttg caagaaacca tagtaaaatc ctattgtaaa cacatatttt
     3601 gggtctcctc ctgctgctat cagtgttgca ttttctgcca tatatgtagc tgctattggc
     3661 atccatatac ttgaagttat tgctgctcca tgaaggccaa agaaccataa taattgagtt
     3721 ataaatagta ttatagttac tgcaaaaaca ttatctacac tgcttatagc tggtgctaag
     3781 aaattcatta taatagctgg taatggttga cctgagacat ttttagttat tgctgctatt
     3841 gctgtaaata acattataca tactatcatt ggagttaaat ttctaaaact tttatttact
     3901 acactaggta cttcttttgg aaatctaatt acgagatttt tatcattcat aaacttagta
     3961 atatcaacta ctaaaagtgc tactaatata cttacaaata aaccttgtgc atctaaataa
     4021 cttgtatcta atacaccatc tactacttgt ccacatacaa caagttgtgc aacaactgct
     4081 gtcataccta tttctacatg attaagatta tattttttag ctagtgcctg agctataaaa
     4141 aatgctgaat atatagataa taaactcata gtaaatttat tagggataag taatgtatcc
     4201 atattttcag ataataatgt ttttgcactt ccttcaggta acatattgtt taatgccata
     4261 aataacatac ttaaagaacc aacaattatt attggggtaa tgcttatcat accttctttt
     4321 atagcttgaa gatgcctttg tttttcaatc ttttgtgcta ttggtagaag aaatcttctt
     4381 aaattatttt gaagtgcttc catttttatt ccccccaatt atctattttt atataattcg
     4441 attgccgact tcataacaga aggagcatcc atacgaccat aatgcttagt atctattaaa
     4501 ccaactgcta ttttgtaatt cttacaagct tcaactacat catcttcctt gaacctaatt
     4561 tgtggtgcta caagtactac atctgtatca ttagcttcaa tgtatgattt taaattttcg
     4621 aatgatgttg cctctatatt tatagtttta gtttttaact tttcatgctc actacaaaaa
     4681 cttttcatct tttccacaag tacacctgtt gaagctccat ttgcacatac taataaaata
     4741 ttcataatta ccctcctaca atatctaacc tcttttattt tctaattttt tatcagccaa
     4801 ttcttactct aattcctatc agtttttact ttaattctta ttattttatt ttaattctta
     4861 ttaatattta ctctaattct tagtactgcc aatgattaaa cttgaaagcc gtatctataa
     4921 cttcttcttg cttatatttt tctaatattt ttaagtatct ttctttataa tcattatcta
     4981 cttctttttc tggtattact atactagctt catttaaaaa gtggaatgaa tcccttccca
     5041 tttctaatcc tctatatgta tgcttgtcaa gtgccacgtc cataacttct ggaactttac
     5101 ccattgcaaa tccttttata attatatttt ttaataaatc acttgatcta tctttctcac
     5161 atgcacaaag atatcttatt gcatgtatga aaaataaagg tctgtctcca tctgcatatg
     5221 aatattcctt tcttacttga tttaaattat ttatgattat tacagctgat gggtccccca
     5281 ttcctatatc ttcaactgat attgtaagta tccttctcca tagcttatct tctaattctg
     5341 gtgatgatat atatagttca taagcaaatt cacaagcttc ttccaccata tttcttctta
     5401 tagctttttg aaatgacgat attatttcgt ctgcactaaa tccgtttctt gtagtcattc
     5461 ttgcccatgg gtcatttgcc tttttatctc ttaatatttt atcttgcata ttatttactc
     5521 ctttttattt aaattttaat tatttatatt actcatttaa taatataagc aagtattatg
     5581 ccagtttttt tagacacttt aaaacactgt tatttcaatc atttttatca tcaaaactta
     5641 gatactttgt ctaagtatat ttatatgtgt ctaatattaa tattatttta gactaaaaaa
     5701 aattaccaaa taagttttta tactcattgg taatcttttt cgattatttc tgcatattct
     5761 aatattaata atatttgata gcactctttt tcattaattt ctatattata ttttttatct
     5821 aattgagata ctatatcttt tattttgctt aggctcttgt gataatcaag tgttttttct
     5881 ttatcaagct tttcaatttc tctagtattt ccaatcaacc catctaccat gaaagctaaa
     5941 tgcatcataa gtccaattac tttatcgtat ttcttttcta tattaaatac taaaaacaat
     6001 tcatcagaca catattttat atctttaaat atcctattat aattagagct ttttaaggat
     6061 gtctttaaac cttcaactac atcatcataa cctatactta tctttataat attttttaat
     6121 ttattgatat tttcatcctt atctatatct tttttgctaa tatagtcaat tcctctaata
     6181 ttaattcttt gttcattgat tactgcaaca atattactac tttctttaag ttttgtaata
     6241 tttaaactta aatctttttc tgttttatat gggacagata taatatctat tccatctaag
     6301 tacctactca tcttttcttc taatattttt cttgttttat tatctacacc ttctgaaaca
     6361 tgtgatgcta taataattgt tttagacaga ttttctttat tttgaatttc attagttcca
     6421 aaataatact ttctcgtgtt agtgatagag ttgtatacgt catttaaagg taatcccata
     6481 ttggccttct gaactgcttc taacacagtt aatgtcatca ccataggaat tactcttact
     6541 tcacactttt tttctttctt tataatttca tcaaaggagt ttaatgaacc catatctgta
     6601 aatagtaata catctttatt gtctatatta ttaattaaat ttttaaaatc tacaataact
     6661 tcatcatagc ttttatctaa cttcatatcg aaagaaatag cactgcactt tccaataata
     6721 tcaccaactg cttttaccat tacttgctgt agtttctcca tgcatagcaa ctataatatt
     6781 aatgttattc actattttat tttgttcatc ttgtttagct tgtactatga ataatgtaat
     6841 atatgctaca gtttctatag ataaaaataa atcaaattca ttttcaatta ttccaactat
     6901 ttttagtgct gttttaaatt cacttctgtt atttcttcta atttgattca aatctatgtt
     6961 gtttgtagag ctacttttat ccaatacgct ttttatataa attgccattg acttaaatat
     7021 tgattcactt atatctattt cttcatcttc tttaataatt tgtcttattg attcacacat
     7081 gcttttaaac ttaacattat taaatagaga agcatctgct atattcattt ctacagattt
     7141 attaagatta gtatttactg cattaataat atttatatca ttgtagttaa taacttcttc
     7201 ttctcctaat gacgtaaata caaaatattc tattccatgt gttataactt catctgcttt
     7261 aatagacttc tctctataat catcactcac ttcatttata gatttatcaa aatatttttt
     7321 ctctatataa actggttctt tattatctct tttatatctt aggtatccat ttgcaacagc
     7381 taattttata tcattttgaa gttgacctac attattttta caatcataaa gtaatagtga
     7441 tttaattatt tctttatgta cttttattga aatagaaata ctttttgatt cttccttgaa
     7501 aaaatttttg actaattcca ttctttcaat cattgtccta tctctcagtg atggtatttc
     7561 tattttcatt ggtattcttc tattgaatgt atctaatagt gttgatgtcg gattttctgt
     7621 agtagcacat attattaata ccttagaatc tctttctttc gttgcatctc ctagtctttt
     7681 aaatgttccc ttatctataa aagtaaacaa catttcttga ccttctgctg gtagtctatg
     7741 aatttcatct aaaaatagta tccctccatt tgcaatttcc aaaagacctt ttctggaaga
     7801 ttctgcacct gtataagctc cttgttctac tccaaaaata tgtcccataa gtaattgtgc
     7861 attattagca taatcagcac aattaaatgt tacaaaagga gctttatttt ttctaattcc
     7921 actatctata gaataatcgt acatatatct agcaatcata ctctttccta ctcctgtttc
     7981 tccatagata atagtatgaa gtccatttgt aggatagagc attgcgacct tgcatttttc
     8041 tattataggt cttaaactat gttctttacc tattagtgtt tcaaaactat ctttttttat
     8101 tttgttttct acttttgttc ctttttttat gccaagcaac tcactttgtg tatcaagcaa
     8161 ttcattcttg ttaaggttaa gatttgcagg gatataatac acaggtcttc cttttatctt
     8221 cgtaaccata ccctcattga ataattggtt taaatatgta cttatattgt ttcttgcaat
     8281 tcctgtcttt tctgataatt ggttagctgt aatccctttt tcattttccc tatatgatga
     8341 ttctaaaact ttcattactt tatctttttt atatatcatt acaagctccc ctacattata
     8401 atattttatt aactaaaacc cctatacaca ctttattttt taataaactc ttattcttta
     8461 actaaggtta taatttatca aactttcctt actttaaatt atagaaaatt attgtagaaa
     8521 aaataagatt attgctcatt ttttctacat tactattttt aattttatag cactttctta
     8581 acaacatact ttacttgttt ttaattttat tatatttgaa tttattatat ttgaaaagta
     8641 gaaataaaaa accctatttt ggagtagttt taaaatttct ccaaaaatag ggttttagtt
     8701 tgtatttcaa ataaatacct gttcaagtat ttttatacta aacaactttt aaattttgtt
     8761 gatatttatt tatttctgat ttagaagaat atacaaaatg tccatctgaa acctcaatcc
     8821 aacttaatga cttttcattt gaacaatcat atccattaga attatactct attctatttc
     8881 ttgacttagc atatctaggg tcaggaacag gaattgcaga aagtagtgat tttgtatatg
     8941 gatgaatagg attattatac aattcatttg aatctgccaa ttctactaat tttccattat
     9001 acataactcc tatacggtca ctaatatgtc tcaccataga taaatcatga gctataaata
     9061 aacaagttaa attcttttct tgttgcaatt ttttaaataa atttactatt tgtgcttgta
     9121 tagacacatc caaagaagct ataggctcat cagctattat aaattctggg tctactgaaa
     9181 gagctcgtgc tatgccaata cgctgtcttt gtccacctga aaaatcagat ggatacttat
     9241 ttgcataact tctatcaagt cctactagct ttagcatctc atatacttta ttcattctat
     9301 ctgttttatt tttacagata ccttgaattt ttagtggctc tgaaattata tctgcaattg
     9361 tcatacgtgg atttaatgat gaagtagaat cttgaaaaat aatctgcata cttttgttta
     9421 catctttttt tataaatttg tacgtttttt tatctgatat gcaatttcct ttgtaaatta
     9481 cttctccacc agtagattca tgtagtctta ttatagtttt accagttgta gattttcctg
     9541 aacccgactc tcctaccaat ccaaatatct cacccttata tatatcaaaa cttatatcat
     9601 caactgcttt tacagtagta cttttgtcca aatgaaaata ttgctttaag ttttttattt
     9661 ccagtatttt ttctctattc attagaaatc acccttccac aatttactct aactggtaca
     9721 tctacttcag gtgcatctgg gtgcaataac caagttgctg cactatgtgt atcattaatt
     9781 ttaaacattg gtggctcttt ttcataatct atcttaagtg catttttatt tcttattgca
     9841 aaagcatctc cttttggtgg gttcaataaa tttggaggca ttccaggtat attatataaa
     9901 tattcatcct gtgaatctaa agtaggtaat gaacccaaca acccccaagt atatggatgt
     9961 ctagggtcat aaaatatatc ctctacagtt cctatttcta caatctttcc tgcatacata
    10021 actgctattc tatcagctat agttgcaact actcctaaat catgagttat aaatattatt
    10081 gacaaaccta ttttatgttg taaatccttt attaaatcaa ttatttgagc ttgtatagtt
    10141 acatctaaag cagttgtagg ttcatcagca atcaatacat ctggattaca ggcaagtgct
    10201 atagctatta ctatacgttg tctcattcca cctgaaaaat gatgtggaaa ttgtttaaat
    10261 cttttctcag gattatcaat tccaaccaat tcaataagct ctatggctct cttttttgct
    10321 tcacttttac ttataccttg atgaatgatt acagcttctg caatttgctt tcctattgat
    10381 atagttggat tgagagatgt catagggtct tgaaatatca ttgatatatt ctttcctctt
    10441 atatcttcca tctctttttc agacttctta accaaatcac ttgatttaag taaaacctct
    10501 ccatttttaa tatatccatt gtatgggagt atcctcatca aactcttaca cagtacagat
    10561 ttcccacaac cagattctcc aactatagcc aatgtctcac ctttttttag attaaaagat
    10621 acatttctta cagcctgaac ttctccttct tctactttaa aacttacaga tagattatta
    10681 acctccaata aatgttccaa aaaccatcaa cccctatgct attcaaacct tagggatata
    10741 tttttatccc taaggtttct aattaatttc agtattattc aattgtccag tcagctatgt
    10801 tccagaatat acctacacca tgatgaccta aaactgtatc aggtgttaat ccttttatgt
    10861 ttggttttcc aacatatatt gcatctatat aagcaataaa tgtataaggc atatccttag
    10921 tcatttctac ttggaattgt ttatataact ttaatttttc atctttatct tctgtttctc
    10981 ttgctttttg aagtatttta tctatagtag gattagagta agcactgtaa tttgcacctt
    11041 tatctgttcc aaatacttta tatgtatggt catctgggtc aaatggactt ccccaaccta
    11101 ttaaatgagc atcttgatta gcccaatctg tttcagttac aacaacagct ttagcatcaa
    11161 caccaatttc ttttaactgt tgtgcacata ttttagccat atcaactcta acttggtcac
    11221 tctcacctgc tgttatctcg aaagctaact tagtaccttc tttttcatag attccatcag
    11281 aacctaattt ccaacctagc ttttcaattt cttgtttagc tttttctgga ttatattcaa
    11341 acttttctat atcaggatta ttatatggtc ccatttgtaa aggtgaatat gctggtacac
    11401 catgtcctaa taatacactg tcaactattg cttttctatc tattgcatag cttaaagcat
    11461 ttggtaatcc tttagctttt ttatctttaa agaatttaga attaaaattg tataatatac
    11521 ctctatagtc ggctgttttc attatattta ctttaaaatt cttttcgtct ttttcaaagt
    11581 tagacatatc tttaggagtt atttgagcta agtctaattc tcctgatttt aattgcattg
    11641 ccttagcctt atcatctgga acaatcttaa agactacttt atctaatcct ggttctttta
    11701 caaaataatc actattctta acaagtgtta tactttgacc tttgtcccat ttttcaagtt
    11761 taaatggtcc agtacctatt ggtttttgat taaattcatc agtagctata tccttacctt
    11821 ccaaaacatg ttttggtaat actcctactg ttaaataatc aagcattgct gtatttggag
    11881 cttttaaagt aatttttata gtattatcat taaccacatc aatttttgtt atatcttcat
    11941 aatttgatgc tatctcagaa gcattatctg gattcattat agtttctatt gtaaacttaa
    12001 catcatttgc cgtaaatttc tctccatcat gccacttaac atcatctctt aaattaaaag
    12061 tatatgtatt tgttgcctca tcaaatttcc agtcctttgc tagacaagga accaccttat
    12121 tattttcatc atgagctgtt aatccattaa atattaatga atttatttct ccatgttcat
    12181 atagtgcagg atttatactt gtatagtcat tacttccata aacaagtacc tttccatctt
    12241 taggcgtatc tgccttctta tctttgtctc ctccactaga acaacctgcc attagactaa
    12301 gtatcattac taaacttaaa acttttaact tctttaattt catgatgctc ccccttttgc
    12361 tttttataaa atatttttca aagctttgac cccatttatt taaatagata tttcaaatac
    12421 ctcagtattt gcttatttag tactcaaatg ctgatttata aattactaga ttttttatta
    12481 ttattctttc ttatataatt acctatattt gttatacaca ccaatgtggt aaccaaaaat
    12541 atacccggaa tcagtataat ccaccatcta tttgaaagta atgcttcctc tgacaaagat
    12601 aacatacttc cccaagaaac aatttctatt ggtaaaccaa ttcctaaaaa gctaagtgtc
    12661 gattcagtac caatagctgc gcctatgttt gttacaacca tgaacattat tgatgccaca
    12721 aaatttggaa gtaaatgttg ctttaataca tagaagaatc ctcctcccat gctcttagct
    12781 gctagtatgt attcgctatt tcttatttgt ctaacttctg ttctcactat cttagatata
    12841 ttcatccaac ttgttatccc tattactatg gacatagata ctggatttga atttccaagt
    12901 atagcttgta caaatattat aattaatatt gaaggtatac taagtatgat ttctgtaaac
    12961 ctcatcattg catcatctac gagttcagat gctgaaccac ttatactacc ataaacaatt
    13021 cctataacag ttgatattac agtggaaaat agtcctataa ataaagatat ttttcctcca
    13081 taccatatca tagaatatat atctctaccc attgagtctg ttccaaataa aaaagtttta
    13141 tttggagcta aattagaact tgctaaatcc atgtatgttg gttcatgtgt cattataaaa
    13201 gatgagaata cacttccaat gactatgatt gataaaataa taattgatat atatggtaat
    13261 tctttaattt ttttagataa ggttttcttt gtttctatat gttcatcttc tgctactaaa
    13321 atataatttt ctcctacaat ttcaaaatca ctatattgtg gcttcattag tatcacctct
    13381 atcatatttc attctagggt ctattttatt gtttataatt tgagctaaca tattcccaaa
    13441 tactactaaa gcccctgtta ttaagcataa aactaataac atattataat catgatactt
    13501 tgcactttca aaacaaagtg ttcctaaccc aggataagaa aaaacttttt caactacata
    13561 agttcctcct agtatatgag gtatcgaaat ggccattata ctaatatatg atggcattat
    13621 atttctcagg caatgcttaa aaacaatagt tctattattt aaaccttttg ctttacataa
    13681 aagcacataa tcttctctta tttcctctag taatttattt cttatcatat atgtgtaata
    13741 ccatagatgg cttaatataa gtactgtaag aggtaagatt agatgtgata tcctacttaa
    13801 tatactggac tcttctccca ttgaataagc accactactt ggaagtatac ttaagtttat
    13861 actaaatata agtatgagta ctaaggcaac ccaaaatgat ggaatacagt tggttattgt
    13921 tcctaatttg catatgattc tatcaatgag cttatcttca tgtaatgtac aaaatattcc
    13981 taacaataag gctagcacaa aggtaagtat ataccctgaa ccacctaata ttatagtgtt
    14041 tatatagaca tcatttataa cagaagtaac attttgttta tatttaaatg aaattccaaa
    14101 ttcccctttt gatgcatttg aaatccattt tatatattga ctgtatattg gttcattcaa
    14161 accaagtttt ttcatagcat tttctttttc ttgtgtactc attctttcta ctccatcccc
    14221 atagtaagag attaatgggt ctcctggagc aagtcttgcc atgtaaaaaa caataaaaga
    14281 taataaaaac attactaaaa taaactgcaa caactttttc agtatatcct ttaacaaacc
    14341 ccaacccccc tttgttgcaa taaaaaaacc acataaaagc aaacgtaaag taacgttaaa
    14401 accttcgtgg ttccattatc attaaacaaa attaaatttt ctatatttat ctatgagtta
    14461 attttagcat catttattaa ctgtgtaaac aaacttttta aaaattgtta aaatttcatc
    14521 aaattatttt atttatacct aaaataaaat ttttatgttc tattcttcat tattttctat
    14581 aaaattataa aattttaggt tgactatata agaaaagtat attatattag tttgtatgca
    14641 aaccatttaa gaggtgattt tattgaaaag taattatgac tgttttagaa tagccatgct
    14701 cttaaaagaa ctttattcta aaactatgta cacagtagaa gaaaatttta aagaaaatgg
    14761 attaacacac caacagatta tagttataaa actggtagct cataatcaag aacttacaat
    14821 atcacaacta tgtgatgaaa tgtctttagc taaaggtacg gtttcaggca taatcagcag
    14881 attagaacag attggatata tagaaaaatt caagaaatct aatgataaaa gaaatacata
    14941 cgttaagttt acaacaacag gatttgaatt tgccacaaat tttaaaatta aaatgcaaga
    15001 aagttttgat gatattttta aaaattgtga tgaaaacgaa ttgagtgact tagtaaaaaa
    15061 tcttagaaat atattggcaa aagtaaagga gagataatta tgaattataa attaatactt
    15121 gcaatagtat ttataacatc agctttaatt ttctacacta taggtgtatt tggagaaaga
    15181 aaggcaaaaa ttttaaaaaa gaaacatgta attatatttt ggcttgggtt tatatttgat
    15241 actttaggaa catttacaat gagtaacata gccaacagcc atacttttga agtaaaatct
    15301 gctttatcac aaaaccttca tagtattaca ggtcttcttg caattgtgct tatgcttttt
    15361 catgcaagtt gggctacttt tgtcctatac aaagatgatg aggaaaagaa aaaattcttt
    15421 cataagttta gtatagttgt atggactata tggttagttc catactttat aggtatgttt
    15481 attggtatgg ttggatagta aaataaaaag tgtgtaagaa atactgtagg taaatttgta
    15541 taagacaaat ttatctacat ccttctttca cacttttaca tatttatcta taatactcca
    15601 ttatttcttt catgtttctt aatacctata tagaatattt aattgtttct atagtatttt
    15661 attattttct cacacttttt catatatata ttgttttact attttttcat atgtatatat
    15721 gaaat
//
LOCUS       DAMJWP010000073        15718 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.077, whole genome shotgun sequence.
ACCESSION   DAMJWP010000073 DAMJWP010000000
VERSION     DAMJWP010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15718
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.077"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            132..1349
                     /locus_tag="PC700_001898"
     CDS             132..1349
                     /locus_tag="PC700_001898"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HDF2581209.1"
                     /translation="MMNVTLTDVKEARETIKNIVKRTDLLESVKLSEKTGANVFYKCE
                     NLQKTGSFKLRGACNKIASLTDEEKANGVIASSAGNHAQGVALGAKMTGIKSTIVMPA
                     TAPLAKVSATKGYGAEVVLNGAVYDDAYAKAVEIQKETGATFLHPFNDKYVIAGQGTI
                     SLEIFEQLDNKVDTILCPVGGGGIISGVAVAAKALNPNVKIVGVQTANIPSMKESIKN
                     GKVTTAFNDTTIADGIAVKTPGDLTFEIINELVDEIVVVEETEIAESILFMMESQKIV
                     SEGAGAVCTAAILSGKYVPAKDENVVCIISGGNIDINTLYRIIGVALAKEGRRYSFST
                     IMEDKPGNFAELTRIISENGGNILSANQGKLSAGEALGKQSAEFILETIDYDHIARIK
                     KAIEEKGFKIIEL"
     gene            1444..1824
                     /locus_tag="PC700_001899"
     CDS             1444..1824
                     /locus_tag="PC700_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDF2581210.1"
                     /translation="MKHEVIHTNDAPAALGPYSQAIKAGNLLFVSGQVPLVPETMEVV
                     EGDVQAQTAQSLKNLKAILAESGADFSNVVKTTVFIKDMNEFGAINEVYAEYFGENKP
                     ARACVEVARLPKDVKVEIEVIAVL"
     gene            2108..2590
                     /locus_tag="PC700_001900"
     CDS             2108..2590
                     /locus_tag="PC700_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2581211.1"
                     /translation="MLNFFKKNKSYKLHAVVSGNSINIEKVNDSVFSKKLMGDGVAII
                     PNSNVVVAPCNGKVTVLTESKHAFGMVSDEGVEILVHIGIDTVNLQGEGFKNEVSQGD
                     TVKKGSPIISFEREKINSQGIDCTTIIIVLNHSEFSEINCMVENEVVAGQDTVIEIMK
                     "
     gene            2611..3426
                     /locus_tag="PC700_001901"
     CDS             2611..3426
                     /locus_tag="PC700_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2581212.1"
                     /translation="MRVVKIFNNNALSTVTNDRKEAILLGLGIGFNKRPGDKVNEDKI
                     EKIYYVQDHMQTKFLELLKNVSPEVMDASQQIISLIPGNEGKFNNKGILSLVEHISFA
                     IDRMKNNVFLPNLMLLDIKMMYSKEFELGVKALEIIYRVCHIHLPEDEAGYIALHFVN
                     LQSNDNLAYDTLKFVKGSIDLIKECYGLELDESSLSTLRFRTHLKFLAQRIFKNEICQ
                     DDKMIEMYDYLINNHPKNKEYLEKLNVYIEKEFRYKLDKPEKIYLLLHLTKIL"
     gene            3477..5027
                     /locus_tag="PC700_001902"
     CDS             3477..5027
                     /locus_tag="PC700_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2581213.1"
                     /translation="MKEKLLGGLETFAKAMVQPLMYLSSAGILMVVGVLLTNDMLLGV
                     LPFFKWEPIQIFGSLIYNCIMAIINNLAVIFAIGIPAALAKKEKHKAALIGFMSYIIY
                     LTASNTMLTSLGQLAKESKMLGLIGTGQATILGIQVVDTSVFGGIILGCIAGYVFNRT
                     SNKTFKGALQIYSGANFSFICMFFVSILIGVVSNFIWPLAQQGIASLANVIKDTGTFG
                     LFLYGFLERILVPTGLHHLIYTPFQFSDLGGVLKVGADTVSGAYAIVMTEMNLPVERF
                     SDSIYYMATGFTKMFGYIGIGAAFIYTAYKQNKKKVKATIIPLIITAVLASVTEPLDF
                     MFVFCAPLLFFVHAVIAGLFIALLKIFDVTALCGGNLLVSIIMNTTVGVEKTHWPVMM
                     ILGLAQIIVYFVVFSFLIKKFNYKTPGREDESTATGEEQSKQLNLDAGIENIIDGLGG
                     KENINTVENCITRLRVNVKDESKINEDIINLTPNSGIVRKGKDIQIIFGLHVHEVRRA
                     VEDFLEAY"
     gene            5124..6476
                     /locus_tag="PC700_001903"
     CDS             5124..6476
                     /locus_tag="PC700_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDF2581214.1"
                     /translation="MIITIVGGGSTFTPGIVKSIALREKELELEEIRLFDIDKERQDK
                     VAVVVNWILKEDVKTDVKLTVTNCEKEAYTDATFIFAQMRVGKYEMREQDEKIPLKYG
                     CVGQETCGCGGMAYGLRTIQPMIKVIDDVEKYAKKDYWILNYSNPAAIVSEACRKLRP
                     NARIINICDMPIAIIDMIAESLNINDVHNIRYDYFGLNHFGWFTSIDYKHRDLMEEIK
                     EYIKENKILLPKSYIENLNKSRSTGNRHAVSSWIHVWESVYTMLESFPKYLPNTYLTY
                     YLLSKETVEHSNINRTRANEVMESREKDLFEGVRHYLETGEISEKVFYAGSHGDWISD
                     LAVSIKNDTRSRFLVITENRGAIPNMPYDAMVEIPAYIGKDGPEVIARGPIPLFQQGL
                     MMQQLNSEKLIVEAYIENSYEKALQAFTLNKTIPSMNVAKEILDEMIEVNKEYWPELR
                     "
     gene            6607..7365
                     /locus_tag="PC700_001904"
     CDS             6607..7365
                     /locus_tag="PC700_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2581215.1"
                     /translation="MNIINKIFWQEDRIGMITNNLEADTHSHCMLQLFLGIEDSIEIT
                     VNEKLVKCNCIIVDKNISHSFSARKKVYYSAIIEPTSIYAEQLTSKMNDFGYWICDND
                     GLEKLRQQGTFLIYNSSKEQYLRFMEMLNNYLNIPITLKHYDDRITELLNLLRTCNCD
                     NHTISSFADKVSLSASRLSHLFKEQIGIPLKSYILFHQMECAFRELLSGKNVTEASMT
                     AGFDTPSHFAGTVKRMMGMPVSLSLKDSEFLKVY"
     gene            7412..8131
                     /locus_tag="PC700_001905"
     CDS             7412..8131
                     /locus_tag="PC700_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HDF2581216.1"
                     /translation="MNKEYLEETKMLNYNEPQLKLLVSSKNWLELDDFHKIKSIYEFV
                     QNDILFGYNASDMLSATQVLNDGMGQCNTKATLLMALLRAVNIPCRLHAFDVTKDFQR
                     GATSKLISLLAPKYILHTWVEVFYQDRWIALEGVITDKKYLEAIQKKFFNHGGTFKKY
                     AIATNDLKNTSIDWDGKDTFIQKEAIVYDYGIFPSPDVFFSTHSQHMSKLKNFIYVHL
                     IRKIMTKNVCKARNNYIDKNE"
     gene            complement(8255..9655)
                     /locus_tag="PC700_001906"
     CDS             complement(8255..9655)
                     /locus_tag="PC700_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDF2581217.1"
                     /translation="METQSNNKKGNLIIAIVMTGAFISSLSQTLLSTALPNIMSDFKI
                     TADIGQWLTTIYLLIAGIIVPTTAYLINRFSTRKLFITSMSIFSIGCIIALFSNNFST
                     MLIARVLQAMGSGSLMPLLQVIILYLCPEEKRGAAMSLVGITVGFAPAIGPTLSGWLV
                     DSFGWHSLFLFLSPIAILDVILSFILLRNVGETQKLKLDIPSIVLSSLGFGGLLIGFT
                     NQGNYGWTNIATYLPILIGIMSLILFTLRQLKSKEPFLELRVFKNKPFLISTILIMIV
                     YASMMSATLMIPLYVQSVRGFSALSSGSLMLPGAILMVVLNPIAGRHLDKYGPHALSI
                     LGTGCLFLGTLSFAFLGRDTSLIHVSLMYCIRMIGISMVLMPLTTWGIKTLDRELISH
                     ATAINNTLRQISGAIGSAILITIMTSATKKAHMSSNMLSNIHGIDVAFSIAATLAFTG
                     LIVSICFIKRHQIIRS"
     gene            complement(9659..10567)
                     /locus_tag="PC700_001907"
     CDS             complement(9659..10567)
                     /locus_tag="PC700_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2581218.1"
                     /translation="MKGDNKVTEKQRLIINIAQKIFDQKGFQNTSISDVVKECKMSKA
                     TFYKHFETKESFICEIINYYDEKFLEIIHSINENNDIPSSEKLNRKIIAVWKNIFSRT
                     TINTYIRENFSEEQRKATSKLQTKSRANLLNEYKLSLFDNYGDKIEKIIFDLVFLLDA
                     LIHQFIYIIHVQKREINVYFIAKFTIQILDLVVENMDNLNPLIEKSMFLHKQEDEVYF
                     SLHNKSLFFKTIQDIEELIKTDTSLLENPKLLEAIKKLYVEGKNQQYDSLIMDAMIAY
                     LEKEDTLRPKVLLLNSIKNQLKKETL"
     gene            10828..11475
                     /locus_tag="PC700_001908"
     CDS             10828..11475
                     /locus_tag="PC700_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF2581219.1"
                     /translation="MKKKICVAIIFALFIVFVEMFIDSSSEGSKIMTAELSKYRKGVN
                     VLSINNIIVANKKYSLPKDYSPQESSEARDAFYKMNKDAQKSGLNLKAFSTYRSYEYQ
                     DRLFKSYVKEHGEKEANRFSAKPGESEHQTGLAFDIGGDDQSCWANKKFNNTKEAKWL
                     YENAYKYGFILRYPEGKENITGYMYESWHYRYVGTEHSKNFAMNNLTLEEYLHIN"
     gene            11800..14439
                     /locus_tag="PC700_001909"
     CDS             11800..14439
                     /locus_tag="PC700_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDF2581220.1"
                     /translation="MSYKSTYQEVIKNLDSSLSGLSKERAEELLEKNGANELKEADKV
                     PTYKLFLESFKDPLVIILLIAALVQIFLGETVESIIIFAVIIINSVLGVVQTKKAESS
                     LESLKNLSAPNAKVIRDNKKMTIPAKNLVVGDIVFLEAGDYIPADGRLIEAQSLKVVE
                     GMLTGESEPVLKHTEKISEDVSLGDQKNMVFSGAVVVYGRGSFVVTGTGMDTEMGKIA
                     GLLESAENKLTPLQQKIEDFSKKLGVGIVILALLIFIIQVARNYFMAGNGEMTQIILD
                     SFMFSVAVAVAAIPEALSSIVTIVLAVGTNSMAKKQAIIRKLPAVETLGSTSVICTDK
                     TGTLTQNKMTVVDYYMYGTNNDELDKEKVNYSYHAKLLTMVSTLCNDSHITSDGKEVG
                     DPTEVALINYSSKNDLDYDKLRSKYIRINEIPFDSDRKLMSTVNSIYGDYIMFTKGAP
                     DIVFSRCKYALKNGSKVDITDEIIEEYKHKNEEFSNRALRVLAFAIKDVPEEDFIPSI
                     DDEHDMTLVGIMAMIDPPREEVMDAVKEAKSAGIKTVMITGDHKTTAAAIAKEIGIME
                     EGDLALTGKELDALSEEQLYEKLENISVYARVSPENKIRIVKAWQHKNNITAMTGDGV
                     NDAPALKQADIGIGMGSGTDVAKDASAMVLVDDNFASIVNAVEVGRTVYSNIIKSITY
                     LFAGNLGAIVSIVFAVFAGWSNPFTALQLLFINLVNDSLPAIALGFEKSEKNIMNKPP
                     RHPDESILAGGTLKAVAIRGCIIGVVTIIAQYIGLQVSPELGVAMAFSTLILARIMQT
                     LPARSNNQTVIKLGFFSNKYVIGAVLVCLGLYSLTLIPVLRGIFSIPTTFGLYQLGIC
                     LGLALISSILMELNKLINRSEVK"
     gene            complement(14497..15600)
                     /locus_tag="PC700_001910"
     CDS             complement(14497..15600)
                     /locus_tag="PC700_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HDF2581221.1"
                     /translation="MKLKINKAIEMQLKKSYAIESGHSEDVFEIDCGEGINTVSYSSK
                     AVEAFNALKFDMIRGYPHSIALKDNIVDYWKDFIALDTDRICLADGSIHVIYLLNRLF
                     IEKGDKVLGYSPQFSEYETDIKMHGATYDYVLLKKEDNFKFNEKEFIEKINPEYKVIY
                     IDNPNNPTGQIIPLSSIENIVKEAAKYDIAVMVDEAYGEYMPKENSAVKLLNNYDNVI
                     ALKTFSKGFGLAGLRAGYAVLPEQLVSPIKKISTPYEVSEISRSIAANLLDDVQFIEE
                     LKEKTKDIKNQLLIPWKNLNIAETSDTVSIMTVEHKNKDIDLQQEFAKLKIRVISGSD
                     FTGLDKNFIRFRMPEEKELPEVIKAFQIIDNIE"
BASE COUNT         5919 a         1897 c         2741 g         5161 t
ORIGIN      
        1 tgtattttta ggttttttgt gattaattaa aaaattataa aaatcgttta catgttgatt
       61 tttaaaaaaa aaagatatag aattatatag aaagtaataa acaaaaattg gatttacggg
      121 agtggaatga aatgatgaat gttaccttaa cagatgtgaa agaggctaga gagaccatta
      181 agaacatagt aaaaagaact gacttacttg aaagtgttaa acttagcgaa aaaacaggag
      241 caaatgtgtt ctataaatgt gagaatcttc aaaaaacagg ttcttttaaa ttaagaggag
      301 cttgtaacaa aatagcgagt ttaacagatg aagaaaaggc aaatggagtt atagcttcaa
      361 gtgcagggaa tcatgctcaa ggagttgcat tgggagcaaa aatgactggt ataaaatcta
      421 caatagtaat gccagcaact gcgcctttag caaaagttag tgcaactaaa ggttatggag
      481 cagaagtagt tttaaatggt gcggtttatg atgatgcata tgcaaaagct gttgaaatac
      541 aaaaagaaac aggagcaact ttcttacatc cattcaatga taagtatgtt atagcaggac
      601 aaggaactat atctcttgaa attttcgaac aattagacaa taaagttgat actatactat
      661 gcccagttgg tggaggagga atcatctctg gtgttgctgt tgcagcaaaa gcattaaatc
      721 ctaatgtaaa aatagtcggt gttcaaactg caaatatacc ttcaatgaaa gagtcaataa
      781 aaaatggaaa agtaacaaca gcatttaatg atactacaat agctgatggt atagctgtta
      841 aaactccagg agatttaact tttgaaataa taaatgaatt ggttgatgaa atagtagtgg
      901 ttgaagagac agaaattgca gaatcaatat tatttatgat ggaaagtcaa aaaatagttt
      961 cagaaggtgc tggtgctgta tgtacagctg ctatattaag tggtaagtat gtaccagcaa
     1021 aagatgaaaa tgtagtttgt ataatatctg gtggtaatat agacataaat acgttatata
     1081 gaataatagg tgttgcttta gctaaagaag gaagaagata ttcatttagt acaattatgg
     1141 aagataagcc aggaaacttt gctgagttaa ctagaataat aagtgagaat ggtggaaaca
     1201 ttttaagtgc taatcaaggt aaattatcag caggggaagc tctaggaaaa caaagtgcgg
     1261 aatttatatt agagacaata gattatgacc atatagctag aataaagaaa gctatagaag
     1321 aaaaaggttt taaaattata gaattataat aaaaagtgta tataatatac aagaaaaata
     1381 gtataatagt gtatgaagat acattttatg tgtattagat taacgaatta ggaggatttt
     1441 aaaatgaaac atgaagtaat acatactaat gatgcaccag cagctttagg accatattct
     1501 caagctataa aagctggaaa cttattattt gtatctggac aagttcctct tgtaccagag
     1561 actatggaag tagttgaagg agatgtacaa gctcaaactg ctcaatcttt aaaaaatctt
     1621 aaagctatat tagctgaaag tggagctgat ttttcaaatg tagttaagac tacagtattt
     1681 ataaaagata tgaatgaatt tggggctata aacgaagttt atgctgaata ttttggagaa
     1741 aataaaccag caagagcttg tgtagaagtt gctagattac caaaagacgt taaagttgaa
     1801 atagaagtaa tagctgtatt ataaaaatac aatattttta atttgaaaag gctgaaaaac
     1861 tatattctag tgattcagtc tttttataaa aaaataaatc acaataaaga acatgataaa
     1921 tattgtcgaa ttaaaaaata aaattaaaat aaatattgca atatttttga gaaggtttta
     1981 taatgagtat ataatttgaa acgtgtaact attaaattag gcatgagttc ttggatattt
     2041 ttatcctgag gattcatgcc ttttttgtat tgaaattcaa aaatctattt aataaggagg
     2101 aagtattgtg ttaaattttt tcaagaaaaa taaaagctat aagttacatg cagtagtgag
     2161 tggtaacagc attaatattg agaaagtaaa cgattctgtg ttttcaaaaa agttaatggg
     2221 agatggagtt gcaattattc ctaatagtaa tgttgttgta gccccttgta atggtaaagt
     2281 aactgtgctt acagaatcaa agcatgcctt tggtatggtc tcagatgagg gtgttgaaat
     2341 attagtccac ataggaattg atacagttaa tttacagggt gaggggttta aaaatgaagt
     2401 gtctcaagga gatactgtta aaaaaggtag tccaataata agttttgaaa gagaaaaaat
     2461 aaattctcag ggaattgatt gtacaacaat aataattgta ttaaatcata gcgaattttc
     2521 agaaataaat tgcatggtag aaaatgaagt tgttgcaggg caagatactg ttattgaaat
     2581 tatgaaataa taggaaggga ttaattcaat atgagagtag taaaaatctt taataataat
     2641 gcattatcta cagtaactaa tgataggaaa gaagctattt tactaggtct tggtataggt
     2701 tttaataaac gtccaggaga taaagttaat gaagataaaa tagaaaagat ttattatgtt
     2761 caagaccaca tgcaaacaaa atttttagaa ttattaaaaa atgttagtcc agaagtgatg
     2821 gatgcttcac aacaaattat aagtcttata ccaggaaatg agggaaaatt taataataaa
     2881 gggatattat ctttagtgga acatattagt tttgcaatag atagaatgaa aaataatgtt
     2941 tttttaccta atttaatgct tttggatata aagatgatgt attcaaaaga gtttgaatta
     3001 ggagtaaaag ctttagaaat tatatataga gtatgtcata tccatttgcc tgaagatgaa
     3061 gcaggttaca tagctttaca ttttgtcaat ttacaatcaa atgataattt ggcatatgat
     3121 actttaaaat ttgtaaaagg tagtattgat ttgattaaag aatgttatgg attagaatta
     3181 gacgaatcga gtttaagtac attacgtttt agaactcatc ttaaatttct tgctcaaaga
     3241 atttttaaga atgaaatttg tcaggatgat aaaatgattg aaatgtatga ttatttaatt
     3301 aacaatcatc ctaaaaataa agaatattta gaaaagttaa atgtatatat tgaaaaggag
     3361 tttaggtata aattagataa acctgaaaaa atttatctac tactacacct aacaaaaatc
     3421 ttataagaat gagatgaatt agtaatagaa atatataatt ttaggggggg ataagattga
     3481 aagaaaaact acttggagga ctggaaacat ttgcaaaggc aatggttcag ccattaatgt
     3541 atttatcgag tgcaggaata ttaatggtgg taggtgtact acttacaaat gatatgttat
     3601 taggagtact tccatttttt aaatgggagc caattcaaat atttggaagc ctaatttata
     3661 actgtataat ggctattatt aataatcttg ctgttatatt tgctataggt attcctgctg
     3721 cactagcaaa aaaagagaag cacaaagcag cgttaatagg atttatgagt tatataatat
     3781 accttacagc ttccaacact atgttaacat cactaggtca actagcaaaa gaaagcaaga
     3841 tgcttggact gatagggact ggacaagcta ctattttagg tattcaagtt gtagatacaa
     3901 gtgtatttgg tggaattatt ttagggtgta tagctggata tgtatttaat cgtacaagca
     3961 acaaaacatt taaaggagct ttacagatat actcgggagc aaattttagt tttatatgca
     4021 tgttctttgt atccatttta attggagttg tgagtaactt tatatggcca ttagctcaac
     4081 aaggaatagc ttctcttgca aatgtcatca aagatacagg tacatttggt ttattcttat
     4141 atggtttttt agagagaata ttagtaccaa ctggacttca tcatttaatc tatacaccat
     4201 ttcagttttc tgatttagga ggagtgctta aagttggtgc agatacagtt tcaggagcct
     4261 atgctatagt tatgacagaa atgaatttac cagttgagcg tttctcagat agtatatact
     4321 acatggcaac tggatttaca aagatgtttg gttatatagg tataggagct gcctttattt
     4381 atactgcata taagcaaaat aagaagaaag ttaaggcaac gattattcca ctaataatta
     4441 cggcagtatt agcctctgta acagaaccat tagactttat gtttgtattc tgtgcaccac
     4501 ttttattctt tgtacatgca gttatagctg gtctttttat agctttatta aaaatatttg
     4561 atgttactgc attatgtgga gggaacttat tagtatcaat tataatgaat actacagtag
     4621 gtgtagaaaa aacacactgg ccagtaatga tgatattagg acttgcacaa attattgtat
     4681 attttgtagt atttagtttt ttaattaaga aatttaatta taaaactcca ggtcgtgaag
     4741 atgagagtac agctacaggc gaagaacaat caaaacaact taatttagat gcaggtatag
     4801 aaaatataat tgatgggtta ggcggaaaag aaaatattaa tacagtagaa aattgcatca
     4861 ctagattacg tgttaatgta aaagatgaaa gtaagattaa tgaagatata attaatttaa
     4921 cgccaaatag cgggattgta aggaaaggaa aagatataca aataattttt ggacttcatg
     4981 tgcatgaggt aagacgtgca gtagaagatt ttttagaagc ttattaaaga tatatattta
     5041 tatagcaagg gtaaataaca gctttaagtg ctgtttacta tttattaaag atacatataa
     5101 tttatagagg aggaataatt attatgatta ttactattgt tggtggagga agcacattta
     5161 ctccaggtat tgttaagtca attgcgttaa gagaaaaaga gttagagctt gaggaaattc
     5221 gcttatttga cattgataaa gaacgccaag ataaagtagc tgtagttgtc aactggatac
     5281 taaaagaaga tgttaaaaca gatgttaaac taactgtaac taattgtgaa aaagaagcgt
     5341 atacagatgc tacatttata tttgcacaaa tgcgtgttgg taaatatgaa atgcgtgagc
     5401 aggatgaaaa aattccattg aaatatggtt gtgtaggtca agaaacttgt ggttgtggag
     5461 ggatggcata cggactgcgt acaatacaac caatgataaa ggttattgat gatgtagaga
     5521 aatatgctaa aaaagattat tggatattaa attactctaa tcctgctgca atcgtttctg
     5581 aagcttgtcg taagttaaga ccaaatgcaa ggattataaa tatctgtgat atgccaatag
     5641 ctattataga tatgatagcg gagtcattaa atatcaatga cgttcataat atacgctatg
     5701 attattttgg tctaaatcat tttggctggt ttacttcaat agattataaa catagagact
     5761 taatggaaga gattaaggaa tacataaaag aaaataaaat attattacca aaatcttata
     5821 tagaaaactt aaataaaagt cgtagtacag gaaatcgcca tgctgtaagt agctggatac
     5881 atgtttggga atctgtttat acaatgttag aaagcttccc aaaatatctg ccaaacactt
     5941 atttaacata ctatctttta agtaaagaaa cagtggaaca ttcaaacata aatcgtactc
     6001 gtgcaaatga ggttatggaa agccgtgaaa aagacttatt tgaaggtgtg agacattact
     6061 tagaaactgg agaaatttct gaaaaagtat tttatgcagg ttcacatgga gattggatta
     6121 gtgatttggc agtttcaatt aaaaatgata cacgtagtcg ttttttagtt ataactgaaa
     6181 atcgtggagc tataccaaac atgccatatg atgcaatggt agaaattcca gcatatattg
     6241 gtaaagatgg tccagaagtt attgctcgag gtccaatacc tctattccaa caaggattaa
     6301 tgatgcaaca acttaacagt gaaaaattga tagtagaagc ttatatagaa aatagttatg
     6361 aaaaagcttt acaagcattc acattaaata aaactattcc aagtatgaat gttgctaaag
     6421 aaattttgga tgaaatgata gaagttaata aggaatactg gccagaatta agatagtagg
     6481 ataaaagaaa gggtttttat ttaataatta gatatgtttt cagagtcttt tatcaaaatg
     6541 tattcacaaa catatggcaa tatattaaaa taaagagtga tatttttaga agaatggggt
     6601 gatgcaatga acattataaa taaaattttt tggcaagaag ataggattgg catgattaca
     6661 aataatttag aggcagatac acatagccac tgcatgttac aactgttctt agggatagaa
     6721 gacagcattg aaataacagt caatgaaaaa ttagttaaat gtaattgtat catagtagat
     6781 aaaaatatct ctcattcatt ttcagcaaga aaaaaagttt attactctgc tattattgaa
     6841 cctacatcta tatatgcaga acagcttacc tcaaaaatga atgattttgg ctactggata
     6901 tgtgacaatg atggattaga gaaattacgt caacaaggaa cttttttgat atataactct
     6961 agtaaagaac aatatttaag atttatggaa atgcttaata actatttgaa tatacctata
     7021 actctcaaac attatgatga cagaattact gaattgctca atttattacg tacttgtaat
     7081 tgtgataatc atactatttc aagttttgca gataaagtgt ccctatcagc aagtagatta
     7141 tcacatctgt ttaaagaaca aatagggata cctttaaaaa gctatatctt atttcatcaa
     7201 atggagtgtg cttttagaga attactttct gggaaaaatg ttaccgaagc ttctatgact
     7261 gctggattcg atactccatc acactttgca gggacagtta agcgaatgat gggaatgcct
     7321 gtttctttat ctttgaaaga tagcgaattt ttgaaagtat attaaatatt ctcgttatat
     7381 aatgaagttg tactaagaaa ggggctttgt tatgaataaa gaatatttag aggaaacaaa
     7441 aatgttaaat tataatgaac cacaacttaa gcttttggta tcatcaaaga attggttgga
     7501 attagatgat tttcataaaa taaaatctat ttatgagttt gtacagaatg atattttatt
     7561 tggatataat gcttctgata tgttaagcgc aacacaggta ttaaatgatg gtatgggaca
     7621 atgcaataca aaagctactc ttttaatggc attattacgt gcagtgaata tcccttgtag
     7681 gcttcatgct tttgatgtaa caaaagattt tcaacgaggt gctacatcca aattgatttc
     7741 actattagca ccaaaatata ttcttcatac ttgggtagag gtattttatc aagataggtg
     7801 gattgctctg gagggtgtca taactgataa aaaatattta gaagcaattc agaagaagtt
     7861 ttttaatcat ggtggaacgt ttaaaaagta tgcaatagct acaaatgatt tgaaaaatac
     7921 atctattgat tgggatggta aagatacttt tatccaaaaa gaagctatcg tgtatgatta
     7981 tggtattttt ccatcaccag atgtattttt ttctactcat tcacaacata tgtcaaaatt
     8041 aaaaaatttc atatatgtac atcttatcag aaaaataatg acaaaaaatg tatgtaaggc
     8101 tagaaacaat tatattgata aaaacgaatg agtgtttaag aggtaactat ataggaaata
     8161 tatagaataa ttaaaactgt actggttaaa atgaataaaa aggagttatc atagagcaaa
     8221 cttaaattgt tcataggata actccttttc tgtattaact tctaataatt tgatgtcttt
     8281 ttataaaaca tatagataca attaaaccag taaatgcaag agtagctgct attgaaaatg
     8341 caacatctat tccatgaatg ttagacagca tgtttgaact catatgagct ttcttagtag
     8401 cacttgtcat tatagtaata agtatagcag aacctatagc tccagatatc tgacggagtg
     8461 tgttattaat tgctgtggca tgagatataa gttctctatc taatgtttta attccccatg
     8521 tagttaatgg cattaaaacc attgaaatac caatcatacg aatacaatac atcaatgaca
     8581 catgaattaa agatgtatct ctgcctaaaa aagcgaagga taaagttcct aaaaacaaac
     8641 atcctgttcc taatattgat agagcatgag gtccatattt atctaaatga cgtcctgcaa
     8701 ttggattgag tacaaccatc aatatagcac caggtaacat taatgaacct gaagataatg
     8761 cagaaaatcc tcttacagat tgaacgtaca gaggaatcat taatgtagca gacatcattg
     8821 aggcatatac aatcataatt aatattgtag aaataagaaa tggtttattt ttaaaaaccc
     8881 tcaattctaa aaaaggttct ttagatttta attgacgcaa tgtaaataaa attaatgaca
     8941 taattcctat taatattggt aaatatgtgg caatattagt ccacccataa ttaccttgat
     9001 tagtaaatcc aattaataaa ccaccaaaac ctagagatga tagtactatt gaaggtatat
     9061 ctaactttag tttttgagtt tccccaacat ttcttaatag aataaatgat agtataacat
     9121 ctaatatagc aataggggaa agaaacagaa ataaactatg ccaaccaaat gaatctacta
     9181 accatcctga taaagttgga cctattgctg gagcaaatcc tactgtaata ccaactaaac
     9241 tcatagctgc accacgtttt tcctcagggc ataaatagag tataataact tgtaaaagtg
     9301 gcattagaga accagaaccc attgcctgca acacacgagc aatcaacata gttgaaaaat
     9361 tattagaaaa aagtgcaata atacatccta tagaaaagat agacattgat gtaataaata
     9421 actttctagt actaaatcga tttattaaat aagctgttgt gggtacaata atacctgcaa
     9481 taagtagata aattgttgtt aaccactgac caatatcagc agtaatttta aaatctgaca
     9541 ttatattagg taatgctgtt gacaatagtg tttgactgag agaacttata aaagcaccag
     9601 tcattacaat tgctattatt aaatttcctt ttttattgtt actttgggtt tccataattt
     9661 ataacgtctc cttttttaat tgatttttaa tgctgttcaa taaaagtact tttggtctta
     9721 aagtgtcttc tttttctaaa tatgcaatca ttgcatccat aattaaagaa tcatattgtt
     9781 gattttttcc ctcgacgtat aattttttta ttgcttctag taatttagga ttttcaagga
     9841 gagaagtatc agttttgatt agttcttcta tatcttgtat cgttttaaaa aatagcgatt
     9901 tattatgaag ggaaaagtaa acttcatctt cttgtttatg taaaaacatt gatttttcaa
     9961 ttaaaggatt tagattatcc atattttcta caactaaatc tagaatttgt atagtaaatt
    10021 tggcaataaa gtatacattt atttctcttt tttgtacatg aataatgtat ataaactgat
    10081 gaattagagc atccaataag aaaactaaat caaatataat tttttctatt ttatcaccat
    10141 agttatcaaa taagcttaat ttgtattcat tcaataagtt agctcgtgat tttgtttgca
    10201 atttacttgt agccttacgt tgctcttcag aaaaattttc tcttatatag gtatttatgg
    10261 tagttctaga aaaaatattt ttccatacag caattatctt tctgtttaac ttttctgatg
    10321 atggaatgtc attattttcg tttatggaat gtataatttc taaaaacttt tcatcataat
    10381 aattgattat ttcgcagata aaactttcct tagtttcaaa atgcttgtaa aaagtagctt
    10441 tagacatttt acactctttt acaacatccg atattgaagt attttgaaaa cctttttggt
    10501 caaaaatttt ttgtgcaata tttataatga gcctttgttt ttctgtcact ttattatctc
    10561 ctttcacgca aaaacgttta gtactatata gtacctacaa gttttaataa atatattata
    10621 catcatttgg aaaaaaaatc aatatataga ctgtaatatg aaatttttga aaaatttgtt
    10681 gttattttta gtcgtataaa aaagaaactt aagagtttct taagaattgt ttaaagaagt
    10741 tgcaataaat tagtggtatg attatagtat gaaagagaat gtcttgaaat ttttctgtat
    10801 caaattccaa ttatggaggt gtagtatatg aaaaagaaaa tttgtgtagc aataattttt
    10861 gctctattta tagtttttgt agaaatgttt attgatagct ctagcgaagg aagtaaaatt
    10921 atgacagctg aattaagtaa atacaggaaa ggcgtaaatg tattatcaat aaacaatatt
    10981 atagtagcga ataaaaaata tagtttgccc aaagattatt ctcctcagga aagcagtgaa
    11041 gcgagagatg ctttttataa aatgaataaa gatgctcaaa agtcaggatt aaatttaaaa
    11101 gcttttagta catatagaag ttatgaatat caagatagat tatttaaatc atatgtaaaa
    11161 gaacatggag aaaaagaagc aaatagattt tcagctaaac ctggagagag tgagcatcaa
    11221 actggacttg cttttgatat tggaggagat gaccagtctt gttgggcaaa taaaaagttc
    11281 aacaatacaa aagaagctaa gtggttgtat gaaaatgcat ataaatatgg ttttatactt
    11341 agatatccag aaggtaaaga gaatataaca ggatatatgt atgaatcttg gcactataga
    11401 tatgttggaa ctgaacatag caaaaacttt gcaatgaata atttgacttt agaagaatat
    11461 ttacatataa attaagagat ggtttgttaa aattaggtta aaataaaaaa taacctagac
    11521 tattagctgt gtaaagtgat ataatgtaaa tacaatttaa gataactact cgaaggggag
    11581 tagcttacac gaaagtggta attaatcgtc aagacagtag aaatactcgg ttaattagca
    11641 agggattcct tgatagcaag accttcaaaa taagcattag gtatgtttgt tttggggtct
    11701 ttttttattc aaggaatttt tatttaaaaa aatttaaatt tcaagtatac ataagagccc
    11761 ccaaacataa aataaattta ttgaagagga gggcaacaaa tgtcttacaa aagtacttat
    11821 caagaagtaa ttaaaaattt agactcaagt ttatctggtt tgagtaaaga aagagcagaa
    11881 gagttgttgg aaaaaaatgg agctaatgaa ttaaaagaag cagataaggt tcctacttat
    11941 aagcttttcc tagaaagttt taaagaccca ttagtaataa ttctattgat agctgcttta
    12001 gtacaaatat ttttaggtga aacagtggag tctataataa tttttgcagt tattattata
    12061 aactctgttc tgggtgttgt acaaacaaaa aaagcagaaa gttcattaga aagtttgaaa
    12121 aatttatctg caccaaacgc aaaggtaata agagacaata aaaaaatgac aattccagca
    12181 aaaaatctag tagttggaga tattgtattc ttagaagctg gcgattacat accagcagat
    12241 ggaaggctta tagaagcaca atctctcaag gttgtagaag ggatgttaac tggagaatca
    12301 gaaccagttt taaagcatac agaaaaaata agtgaagatg taagtttagg tgaccagaaa
    12361 aatatggtat ttagtggtgc tgtagttgtt tatggtagag gttcttttgt agtgactgga
    12421 acaggtatgg atactgagat gggtaaaata gctgggcttt tagaaagtgc tgaaaataaa
    12481 cttacaccat tgcaacaaaa aatagaggat tttagtaaaa aacttggtgt aggtattgta
    12541 atacttgctc ttttaatatt tataattcaa gtagcaagaa attactttat ggctggaaat
    12601 ggagaaatga cacaaattat tttagattca ttcatgttct ctgtagcagt agcagtagca
    12661 gctataccag aagctctatc atcaatagtc actatagtat tggctgttgg aacaaatagt
    12721 atggcaaaaa aacaagccat aataagaaaa ttaccagcag tagaaacttt aggttcaaca
    12781 agtgtaatat gtactgataa aacaggtact cttacacaaa ataaaatgac agtagttgat
    12841 tattatatgt atggaactaa taatgatgag ttagataaag aaaaagtaaa ctattcttat
    12901 catgctaaat tattgactat ggtttctaca ttgtgtaatg actctcatat aactagtgat
    12961 ggtaaagaag ttggtgaccc aacagaagtg gctttaataa attattcaag taaaaatgat
    13021 ttagattatg ataaattaag aagtaagtat atcagaataa atgaaatacc atttgattca
    13081 gacagaaagc ttatgtctac tgtaaatagt atatatggag attatataat gtttacaaaa
    13141 ggtgctccag atattgtgtt tagtagatgt aaatatgctt tgaaaaatgg ttcaaaagta
    13201 gatataacag atgagataat agaagaatat aaacataaaa atgaagagtt ttcaaataga
    13261 gcacttagag tattagcttt tgcaataaaa gatgtcccag aagaagattt tataccttca
    13321 attgatgacg aacatgacat gacattggtt ggaataatgg caatgataga cccaccaaga
    13381 gaagaagtaa tggatgcagt aaaagaggca aagagtgctg gtataaaaac tgttatgata
    13441 actggtgacc ataaaacaac agcagcagca attgcaaaag agattggtat aatggaagaa
    13501 ggagacttgg ctcttactgg aaaagagctt gatgctttga gtgaagaaca actatatgaa
    13561 aaactagaaa atataagtgt atatgccaga gtctcgccag aaaataaaat tcgtatagta
    13621 aaagcatggc aacataagaa taatataaca gcaatgactg gtgatggtgt taatgatgca
    13681 cctgcattaa aacaagcaga tataggaatt ggtatgggaa gtggtactga tgtagcaaaa
    13741 gatgcttctg ctatggtatt ggttgatgat aactttgcaa gtatagtaaa tgctgttgaa
    13801 gttggtagaa cagtatacag taatataata aaatcaatta cttatctatt tgcaggaaac
    13861 ttaggagcta tagtatccat agtatttgca gtgtttgcag gttggtcaaa tccatttaca
    13921 gctttacaac ttttgtttat aaacttagta aatgactctt taccagcaat tgcattaggg
    13981 tttgaaaagt cagaaaaaaa tataatgaat aaacctccta gacacccgga tgaaagtata
    14041 ctagcaggag gaacattgaa ggcagttgct ataagaggtt gtataatagg tgtagttaca
    14101 ataattgcac aatatatagg attacaagta tctccagaac ttggagtagc aatggcattt
    14161 tcaacattga tattagctag aataatgcaa acattacctg cacgttctaa taatcaaaca
    14221 gtaataaaat taggtttttt cagcaataaa tacgttatag gagcagtttt agtctgtctt
    14281 gggctttact ctttaacact aataccagtg ctaagaggaa tattctctat accaacaacg
    14341 tttggattgt accagttggg gatttgccta gggttagcat taatttcatc aatattaatg
    14401 gaacttaaca aattaataaa tagaagtgaa gtaaaataat caaaaaatta aagggcatat
    14461 tgattttaac aatatgcctt ttataaatag tctaatttat tctatattat cgattatttg
    14521 gaatgctttt ataacttcag gtaattcttt ttcttcaggc attctaaatc ttatgaaatt
    14581 tttatcaagt ccagtaaaat cacttccaga tataactctt atttttaatt tagcaaactc
    14641 ttgttgtaaa tctatgtcct tgtttttatg ttcaactgtc ataattgata cagtatcact
    14701 agtttcagct atattaaggt ttttccatgg aattagtaat tgatttttta tatcttttgt
    14761 tttttctttt aattcttcta taaattgaac atcatctaat aaatttgctg caatactacg
    14821 agatatttca gacacttcgt aaggagtaga aatcttttta atagggctaa caagttgttc
    14881 tggaagaact gcatatcctg ctcttagtcc tgctaaacca aatccttttg aaaatgtttt
    14941 taaagcaatt acattatcat aattatttag aagcttgaca gctgaatttt cttttggcat
    15001 gtattcacca taagcttcat ctaccataac tgctatatca tatttagctg cttcctttac
    15061 tatattttca attgatgata aaggtataat ttgtcctgta ggattgtttg gattgtctat
    15121 gtaaattact ttatattctg gatttatttt ttcaataaat tctttttcat taaacttgaa
    15181 attatcctct ttctttaaaa gaacataatc ataagttgca ccatgcattt ttatatctgt
    15241 ctcatattct gagaactgag gtgaatatcc taaaacttta tctccctttt caataaatag
    15301 tctatttaaa aggtaaataa catgtataga accatctgcc aaacaaattc tgtcagtatc
    15361 taatgctata aaatctttcc aataatcgac tatattatct tttaaagcaa ttgaatgagg
    15421 atatcctctt atcatatcaa attttaaagc attaaaagct tctacagctt tgcttgagta
    15481 agacacggta tttatacctt caccacagtc tatctcgaat acatcttctg aatgtccaga
    15541 ttctattgca taactttttt tcaactgcat ttcaatagct ttgttaattt ttagtttcat
    15601 actttatcac tccttattta gtcaataata aatgttataa aagttgctag atttaaaata
    15661 ttatgaaaat atataatagt aaaaaatgaa tataaatatt ataatagctc actttatt
//
LOCUS       DAMJWP010000074        15490 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.078, whole genome shotgun sequence.
ACCESSION   DAMJWP010000074 DAMJWP010000000
VERSION     DAMJWP010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15490
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.078"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            58..876
                     /gene="larE"
                     /locus_tag="PC700_001911"
     CDS             58..876
                     /gene="larE"
                     /locus_tag="PC700_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HDF2581222.1"
                     /translation="MEVNFVKEKEKLDKLKKMLLELGSVVVAYSGGVDSNFLLKVAKD
                     TLGENVIAVTIHAMMHSSREIEEAKQYTQNFGVKHIILNIENFDLKEFKENGVDRCYH
                     CKKYIFSKIKEVAKEHNIKYIVDGTNMDDLGDYRPGLKALSELGVISPLKDSSLKKEE
                     IRSLSKILGLNTFNKPSFACLASRIPYGVEITDENLRIVEKSEEYLFNLGFSQFRVRM
                     HGEIARIEVGQEELGKFFENNNFNKVDTKLKIFGFKYVTLDMSGYKMGSMNLNV"
     gene            931..2079
                     /locus_tag="PC700_001912"
     CDS             931..2079
                     /locus_tag="PC700_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HDF2581223.1"
                     /translation="MKNYTLISPCFFGMEKMLAREITNLGYEIIKTEDGRITYKTDEF
                     GIAKSNMWLRCAERVHLKIAEFEAKSFDELFENTKRINWSRYIPYGAQFPISKASSIK
                     SKLYSTPDVQAIVKKAIVESLKKSYLEDGLLKEDKEKYPIFVFIHKDKVTISIDTTGD
                     ALHKRGYREKANKAPIRETLAAGLIYLTPWKAGRVLVDPMCGSGTILIEAAMIGINMA
                     PGLNREFISEKWRTLDKKIWWDVRKDAFNKIDNESKFKIYGYDIDEESIDIARENAEI
                     AGVDEYIEFNVGDATQFKSEDEFGFIITNPPYGERLEDKDSVKQLYKELGYAFRKLKN
                     WSYYLITSYEDFEYEFGQKADKKRKLYNGMLKTNFFQYPGPKPPRNNK"
     gene            2215..3603
                     /locus_tag="PC700_001913"
     CDS             2215..3603
                     /locus_tag="PC700_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581224.1"
                     /translation="MKVLKLITVVALMMLFVTGCNIKTESPEQLAKIPDYDDTKKVLY
                     DGIDQLLKPDSSMILPSNTKEVGKINKVDLNSDGIDELVVFEQKEDLSNNVSQVGFVT
                     LSYNGEKYVLGDHFLENGESIEYANFYDLDSDGYKEVILLVKSKDKTNMHIYKVVDNE
                     ITKIYDLDASWLQNKEDFNDMKVKIGYIDGDDKLDILMLHLNNKTNEMFASVANFDGK
                     MKIKDSVKFENVKNLSELYTTLGNVASSVGNSSAAIKGIVLDIPILKDNNYITQILYM
                     KDGKINKAFSDYDKTITKSYYIPVDDIDKDKIIEIPIVAGSTGNNKNTYSSKSSATIS
                     WYRWNGKEGADSSLIFTSQIYYNYKYNFKLFIPNNLFDKILVEQEFVGEKAVFKFYYF
                     DYSDRKNLFNIVVESKNKLEDRKNTGTQNSIVLQESDEYTFLLVVNNANEMDKLDMTI
                     DALKEYFSLIYG"
     gene            3632..4330
                     /locus_tag="PC700_001914"
     CDS             3632..4330
                     /locus_tag="PC700_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2581225.1"
                     /translation="MKEKILILEDEIGIRSFVSINLKREGYEIVEAGTGREAIEKMTT
                     ENDITIALLDVMLPDISGIEVCKFIRENFDQVGIIMLTAKAQEDDKIEGFISGADDYI
                     IKPFSIKELLVRVSALLRRVAKDDSSVKSSEIVSPPFILDIDKRKLFKNGKEIELTPT
                     EFSIVKYLISNAKQSLSRDQILDEVWGTNYLYDFKIVDVNIRRIRNKIEDDPSKPKYI
                     QTIWGYGYCFRKEE"
     gene            4333..5766
                     /locus_tag="PC700_001915"
     CDS             4333..5766
                     /locus_tag="PC700_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2581226.1"
                     /translation="MLHFEGLRKKVIKNYFIIIIIMVTLFEGLFMFYIQNYYYDSVKQ
                     LLESEIKYADEYNAITMETTSFEKKVKNIFDKQPLTKNSEFGISIIDKDKNIILDQYG
                     FKSKEKANYEDVNNALKDIKTKNLTPYTYRIPDTGEHVMSISLPLKVNNIIEGVVRYT
                     VSLDAIDNAILKQATWLILAGIFILIIAILISLKFAETLIKPLRELKKFANELAVGNY
                     NIKLEKMKIVDDEIGDLAQTFEHMAHEIDKSEKLKEEFISSVSHELRTPLTSIKGWSE
                     TLGYESITREELDLGLGIIQDETERLIKLVEELLDFSRLSSDRIKLHVDIVDVEGLIV
                     GVVNQLKVKAAEKDISLLFEFENEFIENIQGDKNRLRQVLINLIQNSFKFTSQGGYIK
                     VVASQDEEITTISVEDNGSGIEKQNLNKVLDKFFQEDYNKAGSGLGLAISNEIVKLHG
                     GRMKIESEKNVGTKITFNIKNKFAKQA"
     gene            5788..6525
                     /gene="truA"
                     /locus_tag="PC700_001916"
     CDS             5788..6525
                     /gene="truA"
                     /locus_tag="PC700_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDF2581227.1"
                     /translation="MRNIKMIVAYDGSRYKGYQKLGDNNMTIQEKLENVLSKMTNETV
                     EIIGSGRTDMGAHARGQVVNFRTNCMDSLDKIQKYLYEYLPEDIVVKTVEEVDERFHS
                     RYNVKSKTYMYKIDNNKYHNPFIRKYATHVSKKLDLDRMRKASEYLVGEHDFTSFASS
                     KSKKKSNVREIYSINIKEDDNVIEIYVEGNGFLYNMVRIIVGALIDVGLKRRAPQDIK
                     YMLESKDRCQSSDTAPAKGLCLWKVTY"
     misc_binding    6636..6843
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            7096..8295
                     /locus_tag="PC700_001917"
     CDS             7096..8295
                     /locus_tag="PC700_001917"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HDF2581228.1"
                     /translation="MKEKVVLAYSGGLDTSIIIPWLKENYEDIDVIAVCGNVGQEDKM
                     EDVYEKALQSGASKAYVDDISEEFVTETIFKAVKAEAKYEGKYLLGTSLARPIIAKKL
                     VEVAHKEGAKYICHGCTGKGNDQVRFEATIAALDPTIKVIAPWRIWDIKSREDAIDYA
                     EKHNIKVTATKAKIYSVDANLWHVSTEGGDIEHLENEHKKDVYKQCVDPEDACDVAEY
                     VEVYFEKGVPKKVNGEELSPVALIHKLNELGCKHGIGVIDIVENRLVGMKSRGIYETP
                     GGTILYEAHNILESATLDKDTLHFKQMVSYKYGELIYNGLWYCKLRESIDAFMEQTQD
                     NVTGTVKVKLYKGNIKPAGIFTENALYDEGISSFGNSELYDHKDAEGFINLFTLPLKI
                     RAMKAGK"
     gene            8542..9588
                     /locus_tag="PC700_001918"
     CDS             8542..9588
                     /locus_tag="PC700_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4872 domain-containing protein"
                     /protein_id="HDF2581229.1"
                     /translation="MRKIIEIKHHSHDYECMWNGIEDLYINKTGECLPNDFFFLLSGF
                     GSFCYMKTNKADLKRMVALGDGRTKQMYKFLAPIVGFEYKHHEFKKYEQALKKAKSEI
                     DGGYPVILGALDMYYLPYYKKLYHKEHIPFHYILMVGYDDERIYLYDCGRTELLHLSY
                     DKLRESMNCNSPGLSKENTICTIRMNTSKNKYNIAREAIAIKRDMFLNPSKGFLGYKG
                     FEKFIKELPQWNTELDKDDYDKILTNMVMFFGTVPTIPNAIKGIKEPDTVQFKGGFDK
                     MSRMLNSIGEEYKNDSFIQVADIFDKTAIIIEKISNIIIDYLTQTCDDTEQLPLLFSE
                     VLEHMKTGYLILDV"
     gene            9696..10859
                     /locus_tag="PC700_001919"
     CDS             9696..10859
                     /locus_tag="PC700_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2581230.1"
                     /translation="MRTVKQVSDLTGISVRALHYYDEIGLLKPNKITDAGYRLYDDES
                     IKTLQQILFFKEIDIPLREVKEIMSSQYFDKVEALKNQKKLLILKRKRLDELIELINQ
                     TLRGEGNIDFKEFDMSEYFNVLEEFKREHRNKIIKIYGSVEKYNEYIERVKSNEEKIA
                     KMAIKQYGTIEKFAQAIKTNFSSDILNLGEKFDRYKNDCLKEKHPKLKELYRKLVEDL
                     SKNPSSTDLQEIAKEITDISKKDYEIFSMYTGDDNWYYMIQNYLVNPMWIEEVDKKYG
                     SGAGKFIGQVLKTYLRDRKPKINTLYEKLVEDLSRDCSSREVQSIVEEIDNEMKRSNE
                     FYKIDNGERYFDYMSELYLQDSNYIKVTDKNYGDGASKFIGEAFKIYFDNNNC"
     gene            complement(10948..11328)
                     /locus_tag="PC700_001920"
     CDS             complement(10948..11328)
                     /locus_tag="PC700_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HDF2581231.1"
                     /translation="MKSTTFVILWTILFVLFGFYVNNKLYDFTEGYKDNISVLEKSIE
                     NEDWEKAQKEADSISTSWNKEKNHWYKVLNHEYFDEIGLRFNILDKAIYTENKLKSLE
                     EVESIKTYLGNIIESVKFDVNYIF"
     gene            complement(11331..11990)
                     /locus_tag="PC700_001921"
     CDS             complement(11331..11990)
                     /locus_tag="PC700_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HDF2581232.1"
                     /translation="MTVVLIRSIILYITVLIALRVMGKGEIAEMNCFDLVITLLIAEV
                     ASVPMENNNIPIINGVAAISGLVIMQTLISFLSLKSRKLSSFLSGKPSVLIDKGKIVY
                     KELKKERISIDELLEQLRIQGYFNLKDVQYAILETDGNLSVVPASSYNSTPPRAFNHL
                     PIPLILDGRIINKNLDIAQKDTNWLMGILKSNHIETFKDVLICVLDENDKIFIQNKKG
                     D"
     gene            complement(12096..13070)
                     /locus_tag="PC700_001922"
     CDS             complement(12096..13070)
                     /locus_tag="PC700_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HDF2581233.1"
                     /translation="MKYKLFIDFEFNILDDNKNKPKEYNGAELISIGGVLVDNEFNTI
                     DNYYSLVKPKYNKILSNKCKNLTKLNQLDINNAPNLLYVMDDFYKWFCKFDDVTIYNW
                     GDFDITGLLTSFRVYKYTGKCLELFNMMIDIQPFISQHITYNNRILSKQISLLNMKKI
                     FSVEGDIKHNSLSDAVDLMNVCKCFFFNSPKNIDILEELYNKLPALKTPLYYIPYFED
                     ENFELKFDRTPEDIIIYLKQILTILNISKKEIYFKKRSVLVNNSKIINFKNLSSSFKL
                     IKLNEFEEYPDFILKLGDKKSFVESVIKINKSNRKSIKNLILKLSKKP"
     gene            complement(13110..13457)
                     /locus_tag="PC700_001923"
     CDS             complement(13110..13457)
                     /locus_tag="PC700_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581234.1"
                     /translation="MLNLKEIREEIIKKYIGDSLISELTEELKNILITDLQSNPFKSN
                     IELSIIPANIEIFKKIENILIDSKVNLKMGWERILINYILKDLFELNYEINKSCNEPL
                     MFRVIISIDSYDA"
     gene            complement(13675..14631)
                     /locus_tag="PC700_001924"
     CDS             complement(13675..14631)
                     /locus_tag="PC700_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HDF2581235.1"
                     /translation="MKKILITGALGQIGSELTIKLRNEYGEQNVIASSRRVKEGNPVC
                     ESGIFEILDVTDKNRFFEIAKKYDVDTIIHLASLLSAVAESKPLEAWNLNMNGLINGL
                     EIAKELDCKFFTPSSIAAFGENSPKNMTPQDTLQRPNTIYGVTKVSGELLCDYYHSKF
                     GVDTRGVRFPGLISYVTPPGGGTTDYAVDIYYEALKNKRYKSYIAEGTKMDMMYMPDA
                     LQSIVDLIQAPADKLIHRNAFNITAMSFSPEEIADSIKKYIPDFVIEYDVDPVRQSIA
                     DSWPNSLDSSSAVKEWNFKFSYDLDKMTKDMLEKLSEKGIGK"
     gene            15072..15440
                     /locus_tag="PC700_001925"
     CDS             15072..15440
                     /locus_tag="PC700_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HDF2581236.1"
                     /translation="MTRPSIYHSQGYTCAEALIKSYNEEHNTDIPISIGSGMGVGMNV
                     GSVCGAVNAAVVIIGYLNGRNSNLDENIARKYSRELLNKVRNKYSSEICVSLKKNKVT
                     CEEIINFTYDALNDMLKNQK"
BASE COUNT         6086 a         1662 c         2533 g         5209 t
ORIGIN      
        1 aatcattaag aatgtaaaaa aataatatat agttttatag ttcatatttt tccagatatg
       61 gaggtaaatt ttgtgaagga aaaagaaaaa ttagataaat taaaaaaaat gttacttgaa
      121 ttaggcagtg ttgtagttgc ttattctggt ggagtagata gtaatttttt gcttaaggtc
      181 gcaaaagata cattaggtga aaatgttata gctgtgacca ttcatgcaat gatgcattca
      241 agtagggaaa ttgaagaggc taaacaatat actcaaaatt ttggcgttaa acatatcata
      301 ttaaatatag aaaactttga tttaaaagaa tttaaagaaa atggtgttga tagatgttat
      361 cattgtaaaa aatatatatt ctctaaaata aaagaggtag caaaagaaca taatataaag
      421 tacatagtag atgggacaaa tatggatgat ttaggagact atagacctgg gcttaaggca
      481 ttgagtgaat taggagtgat tagtccatta aaagatagta gtttaaaaaa agaagaaatt
      541 aggtcgcttt caaaaatatt aggattaaat acttttaata aaccatcatt tgcatgtctt
      601 gcaagtagga ttccttatgg agttgagata acagatgaaa atttaagaat agtagaaaag
      661 agtgaagaat atctattcaa tttaggtttt tctcaattta gagtgagaat gcatggagag
      721 atagctagaa tagaagttgg gcaggaagaa ctaggtaaat tttttgaaaa taacaatttt
      781 aataaagttg atactaaatt aaaaatattt ggctttaaat atgttacact agatatgtca
      841 ggatataaaa tgggaagtat gaatttaaat gtgtagatta ttttaaaaat aaataagttt
      901 tatagttaat ataaggaaac aggaggtttt atgaaaaatt atacattaat atcgccatgt
      961 ttttttggaa tggaaaaaat gctggctaga gagattacta atttaggata tgaaataata
     1021 aaaacagaag atggaagaat aacatataaa actgatgagt ttggaatagc aaagtcaaac
     1081 atgtggttga gatgtgctga aagagtacat cttaaaattg ctgaatttga ggcaaaaagc
     1141 tttgatgaat tatttgaaaa tacaaaaaga ataaactggt ctagatatat accttatgga
     1201 gctcaatttc ctatatcaaa agcttcctct ataaaatcaa aattatacag tacaccagat
     1261 gtacaagcca tagtaaaaaa ggctatagta gaaagcttaa agaaaagtta tttagaggat
     1321 ggtttgctta aagaagataa ggagaaatac cctatttttg tatttataca caaagataaa
     1381 gtaaccattt ctatagatac aacaggagat gctttgcata agagaggata tagagaaaag
     1441 gcaaataaag ctccaataag agagacttta gctgctgggc ttatttattt aactccctgg
     1501 aaagcaggta gagttttagt agaccctatg tgtggttctg gtactatatt gattgaagct
     1561 gctatgattg gtataaatat ggcacctggc ttaaatagag agtttatatc agaaaagtgg
     1621 agaactcttg ataagaaaat atggtgggat gtaagaaaag atgcttttaa taagatagat
     1681 aatgaatcta aatttaagat ttatggatat gatatagatg aagaatctat agatatagct
     1741 agagaaaatg ctgaaatagc aggtgtagat gaatatatag agtttaatgt aggagatgca
     1801 actcaattta aaagtgaaga tgagtttgga tttataatca ctaatcctcc ttacggagaa
     1861 agattagaag ataaagacag tgttaaacag ttgtataagg agcttggtta tgcatttaga
     1921 aaattaaaaa attggtcgta ttatttgata acttcttatg aagactttga atatgagttt
     1981 gggcaaaaag cagacaaaaa aagaaaattg tataatggta tgttaaaaac taactttttc
     2041 caatatccgg gtcctaagcc tcctagaaat aataaataag ttttacatga tttatttgta
     2101 aaatataaaa ttttattaaa ataagtaaaa cattatgatg ccttgaaacg tctaattatt
     2161 gtgaggagat ttttgtgaac aaatttaaag atttatagga ttaggaggag ttaaatgaaa
     2221 gtgttaaagc ttattactgt agtagccctg atgatgttat ttgttactgg atgtaatata
     2281 aaaactgaat ctccagaaca attagcgaag atacctgact atgatgatac aaaaaaagta
     2341 ttgtatgatg gtattgacca gttactaaaa ccagattcat ctatgatatt gccttctaat
     2401 acaaaagaag taggaaaaat aaataaggta gatttaaaca gcgatggaat tgatgaatta
     2461 gtagtatttg aacaaaaaga agatttaagt aataatgtga gtcaggtagg gtttgtaacc
     2521 ttgagttaca atggagaaaa atacgtactt ggagaccatt ttttagaaaa tggcgaatca
     2581 atagaatatg ctaattttta tgatttggac tcagatggat ataaggaagt tatcttattg
     2641 gttaaatcca aagacaaaac gaatatgcat atatataaag tagtagataa tgaaataaca
     2701 aaaatatatg acttggatgc atcatggctt caaaataaag aagattttaa tgacatgaaa
     2761 gtaaaaattg gatatataga tggagatgac aagttagaca tattgatgct tcatttgaat
     2821 aataagacta atgaaatgtt tgcaagtgtt gcaaattttg atgggaaaat gaaaatcaaa
     2881 gattctgtta agtttgaaaa tgtaaaaaac ttaagtgaat tatatacaac attaggtaat
     2941 gttgcttcat ctgttggaaa ttcttctgca gctataaaag gtattgtact tgatattcct
     3001 atattgaaag ataataacta tattacgcag atactttata tgaaagatgg gaaaattaac
     3061 aaagctttta gtgattatga taaaactatt actaaatctt attatatacc tgtagatgat
     3121 attgacaaag ataagattat agaaatacct atagttgcag gcagtacagg taataataaa
     3181 aatacttata gttctaaatc atctgcaact ataagttggt atagatggaa tggaaaagaa
     3241 ggtgctgact caagtttaat atttacaagt cagatatatt ataattataa atataatttt
     3301 aaattattta ttccaaataa tttatttgat aaaatacttg ttgaacagga atttgttgga
     3361 gaaaaagcag tatttaaatt ttactatttt gactattcag atcgtaagaa tttatttaat
     3421 atagtagtag agagtaaaaa caaacttgaa gatagaaaaa atacaggtac tcaaaactct
     3481 attgtacttc aagagagcga tgaatatact ttcttactag ttgtaaacaa tgcaaatgaa
     3541 atggataagc tagatatgac tatagacgca ttaaaagaat atttctcatt aatatatggt
     3601 taatattata ctcaattgga ggttttattt tatgaaggaa aagatactta tactagaaga
     3661 tgaaattggt attagaagtt ttgttagtat aaacttaaaa agagaaggct atgaaatagt
     3721 tgaggcaggt actggaagag aagctataga aaaaatgaca acagaaaatg atattacaat
     3781 agctctttta gatgtgatgc ttccagatat aagtggtata gaagtatgta aatttataag
     3841 ggagaatttt gaccaagtag gtataataat gcttactgct aaagctcaag aggatgataa
     3901 aatagaagga tttatttcag gagcagatga ttacatcata aaaccattta gtataaaaga
     3961 gcttttagtc agggtttctg ctcttcttag aagagtagca aaggatgatt caagtgttaa
     4021 gtcgagtgaa atagtttctc caccatttat acttgatata gacaagagaa aattatttaa
     4081 aaatggaaag gaaattgagc ttacaccaac tgaattttca atagtgaaat atctaatatc
     4141 aaatgctaag caatcgttaa gtagagacca gatattagac gaagtatggg gaactaacta
     4201 tttatatgat tttaaaatag tagatgtaaa tataagaagg ataagaaata aaatagaaga
     4261 tgacccatca aagccaaaat atatacaaac tatttggggt tatggatact gttttagaaa
     4321 ggaagaataa tagtgttaca ttttgaaggt ctgagaaaaa aagtaataaa aaactatttt
     4381 ataataatta ttataatggt tactctgttt gaaggattgt tcatgtttta catacaaaac
     4441 tactattatg attctgtcaa acaactactt gaatctgaaa taaaatatgc tgatgaatat
     4501 aatgctatta caatggaaac tacaagtttt gagaaaaaag taaaaaatat atttgataaa
     4561 caaccattaa ctaaaaattc agaatttgga atttcaataa tagataaaga taaaaatatt
     4621 atattagacc aatatggttt taaaagtaaa gaaaaagcaa actatgaaga tgttaacaat
     4681 gcattaaaag atataaaaac taaaaattta acaccttata cttatagaat accagataca
     4741 ggggagcatg ttatgagtat ctccttacca cttaaggtaa ataatataat agaaggtgtt
     4801 gttagatata cagtatcatt agatgctatt gataatgcaa tattaaagca agctacatgg
     4861 cttatattag caggaatatt tatacttatt atagctatac taataagtct taaatttgct
     4921 gagactctta taaaaccatt acgagagcta aagaaatttg caaatgaatt ggctgtagga
     4981 aattataata taaaattaga aaagatgaag attgtagatg atgagattgg tgatttagca
     5041 cagacatttg aacacatggc acatgagatt gacaaaagtg aaaaattaaa agaggaattt
     5101 atatcatctg tatctcatga actaaggact cctcttacat ctataaaggg gtggagtgaa
     5161 accttaggtt atgaaagtat aacaagagaa gaactagact taggtttagg cattatacaa
     5221 gatgaaacag aaagacttat aaaactagtt gaagaattat tagacttttc aaggctttct
     5281 tcagacagga taaaacttca tgttgatata gttgatgttg aaggtttaat tgtaggtgtt
     5341 gtaaatcaac ttaaagttaa agcagctgaa aaagatatat cacttttatt tgaatttgaa
     5401 aatgaattta tagagaacat tcaaggagat aaaaatagat taagacaggt tctcataaat
     5461 ttaatacaaa attcatttaa atttacgagt caaggtggtt acataaaagt tgtagcttct
     5521 caagatgaag agataacaac catatcagta gaggataatg gttcaggtat agaaaagcaa
     5581 aatttaaata aagtgttaga taaatttttc caagaagatt ataataaggc tggtagtgga
     5641 ttaggattag caattagtaa tgaaattgta aaacttcatg gtggaagaat gaaaatagaa
     5701 agtgaaaaga atgttggaac aaaaataact tttaatataa aaaataaatt tgctaaacaa
     5761 gcataaattc ttagaaagag gattaagatg agaaacataa aaatgatagt agcatacgat
     5821 ggttcaagat ataaaggata tcaaaaatta ggtgacaata atatgactat tcaagaaaaa
     5881 cttgaaaatg ttttaagtaa aatgactaat gaaacagtgg agataatagg gtctggaaga
     5941 actgatatgg gagctcatgc aagaggacaa gtggtaaatt ttagaactaa ttgtatggat
     6001 tctttggata aaattcaaaa atatttatat gaatatttac cagaagatat agttgtaaaa
     6061 actgttgaag aagttgatga aagatttcat tcaagataca atgtaaaatc aaagacttat
     6121 atgtataaaa ttgacaataa taagtatcac aatcctttta taagaaaata tgctactcat
     6181 gtaagtaaaa aattagattt agatagaatg agaaaagcaa gtgagtattt agtaggagaa
     6241 catgatttta ctagttttgc ttcttctaaa tctaagaaaa aatcaaatgt aagagaaatt
     6301 tattctataa atataaaaga agatgataat gttatagaaa tatatgtaga aggaaatgga
     6361 tttttgtaca acatggtaag gataatagta ggtgctttga tagatgttgg tctaaaaaga
     6421 agagctccac aagatattaa gtatatgctt gaatcaaaag atagatgtca atcatcagat
     6481 actgcaccag ctaaaggact atgtctttgg aaagtaacat attagtcaat tatataaaac
     6541 tctctttaaa ataaaaataa aataaattat atagttgaca taaatggaaa aataaactat
     6601 tatatatttt ataagagaga tataaaactt tatatttaaa tcctgtgaga aagagagtaa
     6661 cttctatgtg ttttatagag agctgaggat ggtggaagct cagcaaataa gtattagtga
     6721 aaagaacttt ggaggatact aattgaattt agtatgttag taggtaatct cgccttaaga
     6781 gaaagagtgg ataagtttat cttatcaatt agagtggtaa cgcggatata tttcgtctct
     6841 tggtttttcc aagaggcgtt ttttaatata tttaattatt agtgtttatc tggtatgttg
     6901 aacatttact aattgattag ctaagtttat caataagagt ggtaccgcgg atatttcgcc
     6961 tcttagtatt ttaaaatact aaggggtttt tttagtatta aaatgatttt atgtgagtct
     7021 aaattctcag agtcattcaa tattaagaat ttctataaaa taaaaattat aatttaagat
     7081 tgggaggaat taaaaatgaa ggaaaaagta gtattagcat attcaggagg acttgacaca
     7141 tcaatcataa taccttggtt aaaggaaaat tatgaagata tagatgttat agctgtttgt
     7201 ggaaatgtag gtcaggaaga taaaatggaa gatgtatatg aaaaagcttt acaaagtgga
     7261 gcatcaaagg catatgtgga tgatataagt gaagaatttg taactgaaac tatatttaaa
     7321 gccgtaaaag cagaggctaa atatgaagga aaatacttat taggaacttc tttagcaaga
     7381 cctataatag ctaaaaaatt agttgaagtt gctcataaag aaggtgcaaa atatatatgc
     7441 catggatgta caggaaaagg taatgaccaa gttagatttg aagctacaat tgcagctctt
     7501 gacccgacta taaaagttat tgcaccttgg agaatttggg atataaaatc aagagaagat
     7561 gctatagact atgctgaaaa acataatata aaggtaacag caactaaagc taaaatatat
     7621 tcagtagatg ctaacctttg gcatgtttca acagaaggtg gagatataga gcacctagaa
     7681 aatgaacata aaaaagatgt ttataaacaa tgtgtagacc cagaagatgc atgtgatgta
     7741 gctgaatatg tagaagttta ctttgaaaaa ggtgtaccta aaaaagtaaa tggcgaagaa
     7801 ttatctccag ttgcactgat tcataagtta aatgagttgg gttgtaaaca tggaattggt
     7861 gttatagata tagttgagaa tagacttgtt ggtatgaaat caagaggtat atatgagact
     7921 ccaggaggaa caatccttta tgaagcacat aatattttag aaagtgctac tttagacaaa
     7981 gatacattac atttcaaaca aatggtatct tataaatacg gagaacttat ttacaatgga
     8041 ctatggtatt gtaaattaag agaatctata gatgcattta tggaacaaac tcaagataat
     8101 gttacaggaa cagtaaaagt taaactttac aaaggaaata taaaacctgc tggaatattt
     8161 actgaaaatg ctctttatga tgaaggaata tcttcttttg gaaatagtga actttatgac
     8221 cacaaagatg cagaaggatt tattaattta ttcactttac cattaaaaat cagagctatg
     8281 aaagctggaa aataataaaa taaattattt aagagtgtat aaaaactatt tttaggtttt
     8341 tatatgctct ttttttattt gaatttatta aattatacag taaatagtat aaaaaaatat
     8401 attatagttc caaatgactt aataatgtga aaatataaga cctaaaatat aaattttata
     8461 aatacaatag ttattgactc tctcattgta agagggttta tactgaatct aatgaaaatt
     8521 tatagtgagg agatagatta tatgagaaaa ataattgaaa ttaaacatca ttcacatgat
     8581 tatgagtgta tgtggaatgg tatagaagat ttatatataa ataaaaccgg tgaatgcctt
     8641 ccaaacgatt ttttcttttt actgtcaggt tttggttcat tttgttatat gaaaacaaat
     8701 aaagctgatt tgaaaagaat ggtagctttg ggagatggaa ggacaaaaca gatgtataaa
     8761 tttttagcac caattgttgg atttgaatac aaacatcatg agtttaaaaa gtatgagcaa
     8821 gcattaaaga aagcaaaatc tgaaattgat ggtggatatc cagttatttt aggagcttta
     8881 gatatgtatt atctaccata ttataagaag ctatatcata aagaacacat tccttttcat
     8941 tatatattaa tggtaggata tgatgatgaa agaatatatc tgtatgattg tggtagaaca
     9001 gaattattac atctttctta tgataaatta cgtgaaagca tgaattgcaa ttcccctgga
     9061 ttaagcaaag aaaatactat ttgcacaata agaatgaata cttcaaagaa taaatataac
     9121 attgctagag aagctattgc tataaagcgt gatatgtttt taaatccatc taaaggattt
     9181 ttgggatata aaggttttga aaagtttatt aaagagttgc cacagtggaa cactgaattg
     9241 gataaagatg attatgataa gattttgacc aatatggtta tgttttttgg aacagtacca
     9301 acaattccaa atgcaatcaa aggaattaaa gaaccagata ctgttcaatt taaaggtgga
     9361 tttgataaaa tgagtagaat gctcaacagt attggggagg aatacaaaaa cgactctttt
     9421 atacaagtag ctgatatttt tgataaaact gctattataa ttgagaaaat atccaatatt
     9481 ataatagatt atttaactca aacatgtgat gatacagaac aacttccgtt attgttctct
     9541 gaggtcttag agcatatgaa aacaggatat ttaatcttgg atgtataaaa aatatattgt
     9601 tgaccctaac attacgtaat agtgtaatat gaaaatatag cttgaatata ttagtaggta
     9661 ttgatggctg taaaattaaa atttgaggtg tggatatgag aacagtgaaa caagtttctg
     9721 atttaactgg aataagtgtg agggcattac attattatga tgagataggt cttttaaaac
     9781 caaataaaat tacagatgca ggatatagac tttatgatga tgaatctatt aaaactcttc
     9841 aacaaatttt attttttaag gagattgata ttcctttaag agaggttaag gaaataatgt
     9901 caagccaata ttttgataag gttgaagctc taaaaaatca aaaaaaatta cttattttaa
     9961 aacgaaagag actggatgaa ttgatagagc ttataaatca aactctaaga ggagaaggaa
    10021 atattgattt taaagagttt gatatgagcg agtattttaa tgttttagaa gaatttaaaa
    10081 gagaacatag gaataagata attaaaattt atggtagtgt agaaaaatac aatgaatata
    10141 tagaaagagt taagtctaat gaagagaaaa ttgctaaaat ggctataaaa caatatggaa
    10201 ctattgagaa gtttgcccaa gctataaaaa caaattttag cagtgatata ttgaatttag
    10261 gagaaaaatt tgatagatat aaaaatgatt gtttaaaaga aaaacatcca aaattaaaag
    10321 aattatatag aaagcttgta gaagatttaa gtaagaatcc ttcttcaaca gaccttcaag
    10381 aaatagcaaa agagataaca gatatatcta aaaaagatta tgaaattttc tctatgtata
    10441 ctggtgatga caactggtat tatatgattc aaaattatct agtaaatcct atgtggatag
    10501 aagaagtaga taaaaagtat ggaagtggag caggcaagtt tattggacaa gtactaaaga
    10561 cttatctaag agatagaaaa ccaaagataa atacattgta tgaaaaactt gtagaagatt
    10621 tgagtagaga ttgttcttca agggaagttc aaagcattgt tgaagaaata gacaatgaaa
    10681 tgaaaagaag taatgaattt tataaaatag acaatggtga aagatacttt gattatatgt
    10741 cagagcttta tttacaagat tctaactata taaaagtaac tgataagaat tatggtgatg
    10801 gtgcatctaa gtttattgga gaagctttta agatttattt tgataataat aattgttgaa
    10861 aatttggaag taaaaaatat gtaagtttta taaaagcaca aaaataagga tgtagattag
    10921 ctccatctat atccttattt aaagttatta aaatatatag tttacatcaa acttaacact
    10981 ttcaataata tttcccaaat atgtttttat actttcaact tcttctaaag attttaattt
    11041 attttcagta taaattgctt tgtccagaat attaaacctt aatcctattt catcaaagta
    11101 ctcatgattt aaaactttgt accaatggtt tttttcttta ttccaactcg tagatattga
    11161 atcagcttct ttttgagctt tttcccaatc ttcattttct atagattttt ctaaaacaga
    11221 tatattgtct ttatatcctt ctgtaaaatc atatagttta ttgttaacgt aaaatccaaa
    11281 taatacaaat aagatagtcc atagtataac aaatgttgta gatttcaata ttaatcaccc
    11341 tttttatttt gaataaatat tttgtcattt tcatctaaaa cacaaataag aacatcttta
    11401 aaagtttcta tgtgattaga ttttaaaata cccattaacc agttagtatc tttttgagct
    11461 atgtctagat ttttatttat tattctacca tccagtataa gtgggatagg taaatgatta
    11521 aaagctcttg gaggagtact attgtagctt gatgctggta caacacttag attaccatca
    11581 gtctccaaaa tagcatattg tacatctttt aggttgaagt atccttgaat cctaagttgt
    11641 tctaataatt catctatact aattctttct tttttaagct ctttatatac aatttttcct
    11701 ttatctatta atacagaagg tttacctgat aaaaatgaac ttaattttct gctttttaat
    11761 gataagaatg aaataagagt ctgcatgatt acaagaccag atattgctgc aacaccattt
    11821 atgattggta tattattatt ttccattggg acagatgcaa cttctgcaat taatagtgtt
    11881 ataactaaat caaaacagtt catttcagct atttcacctt ttcccatgac tctaagtgca
    11941 attagcactg ttatgtataa tataatactt cttattaata ctactgtcat atctatcaat
    12001 tactccttta aaaaatattt acacttagtt agtatatcct atataaaaaa tttaattcaa
    12061 agtataaaaa accttaaata attggattca actatttaag gtttttttga taattttaat
    12121 attaaatttt ttatggattt tctgttactt ttatttattt taataacaga ttcaacaaaa
    12181 ctctttttat ctcctaattt taagataaaa tcaggatatt cttcaaattc attgagtttt
    12241 attaatttga aagaagaaga tagattttta aagttaatga ttttagagtt attcactaat
    12301 acacttcttt ttttaaaata aatttctttt ttacttatat ttagtatagt taatatttgt
    12361 ttaagatata ttattatatc ttctggagtt ctatcaaatt tgagttcaaa attttcatct
    12421 tcaaagtagg gaatataata taaaggtgtt ttgagagcag gtaatttatt atataattct
    12481 tcaagaatat cgatattttt aggggagtta aagaaaaagc atttacatac gttcattaaa
    12541 tccacagcat ctgaaagtga gttatgttta atatctcctt ctacagaaaa aatttttttc
    12601 atattcagta aagatatttg cttagataag attctattat tgtatgtaat atgttgagat
    12661 ataaaaggtt gaatgtcaat catcatatta aataactcta agcatttacc tgtatattta
    12721 tatactctaa atgaagttag aaggccagtt atatcaaaat caccccaatt atatattgta
    12781 acatcatcaa atttacaaaa ccatttataa aaatcatcca taacatataa aagattagga
    12841 gcattattga tatctagttg gttcaatttg gttaaatttt tacatttatt tgaaagtatt
    12901 ttattgtatt ttggcttaac taaagagtaa taattatcta ttgtattaaa ttcgttatca
    12961 actaaaactc caccaataga aattaattcc gcaccattat attcttttgg tttattttta
    13021 ttatcatcta gtatattaaa ttcaaagtct ataaataact tgtatttcat aaatctaaag
    13081 cacatcctca ggtaaattat ttattgagcc taagcatcat aagaatcaat agatataatg
    13141 actctaaaca tcaaaggttc attacaactt ttatttattt cataatttaa ttcaaataaa
    13201 tcttttaaga tataatttat aagtattctt tcccaaccca tttttaaatt aactttagag
    13261 tctattaata tattttctat ctttttaaat atttctatat ttgctggtat tatactgagc
    13321 tcaatattac ttttaaacgg gttagactgt aaatctgtta taagtatatt ttttaattcc
    13381 tctgtaagtt cagaaattaa actatcacct atgtattttt ttatgatttc ctctctaatt
    13441 tcttttaaat ttagcaatat tagtacctgc tacttacata gtagcttttc ctttcctttt
    13501 aatactatta ttcatttttg aatcttattt agatatattt taagattaaa agtctttagc
    13561 tatataattt tttacccatt tttaacttat attaacatat tacaggctta tatctccacc
    13621 ctgcttacat tgaggatgga gtatattgcc tattttgata aaaatgatag gttgttattt
    13681 gcctattcct ttttctgata atttttctaa catatctttg gtcattttat ctaaatcata
    13741 actaaactta aagttccatt cttttactgc actactactg tctagagagt taggccaaga
    13801 atcagctata ctttgtctta cagggtctac atcatattct atcacaaaat caggaatata
    13861 tttttttata gaatcagcta tttcttcagg agaaaagctc atagcagtaa tattaaaagc
    13921 atttctatgg atgagtttat ccgctggagc ttggattaaa tccactattg attgaagtgc
    13981 atcaggcata tacatcatat ccatttttgt accttctgct atgtaagatt tatatctctt
    14041 attttttaat gcctcataat atatatcaac tgcataatct gtagttcctc caccaggagg
    14101 cgtaacataa gaaataagac ctggaaatct aacacctcta gtgtctactc caaattttga
    14161 gtgataataa tcacatagta attctccaga aactttagtc acaccatata tagtattagg
    14221 tctttgtaat gtatcttgag gagtcatatt ttttggagaa ttttcaccaa aagcagcaat
    14281 tgaactaggt gtaaaaaatt tacaatccaa ttctttggct atttcaaggc catttattaa
    14341 tccattcata tttaagttcc aagcttctag gggtttactt tcagcaactg cagaaagtaa
    14401 agatgctaga tgaattatag tatctacatc atattttttt gcaatttcaa aaaaacgatt
    14461 cttatctgta acatctaata tctcaaatat accagattca catactggat taccttcttt
    14521 tactctcctt gaactagcga ttacattttg ttctccatac tcatttctaa gctttattgt
    14581 taattctgaa cctatttgtc ctaatgcacc tgttataagt atttttttca attttacaac
    14641 ctcctaaggg tttaatttat gtagtactac ttatttttat tttagcaaat cttttgttta
    14701 aatacaaaga attaaagata ataatatacg acttaattga atgaagtgca gtaaattcct
    14761 aaaaatttaa agttgagata taatggtcta ttgtataaca ataaaagact agattaaatt
    14821 attatataga ttgtgtgact aagtcacaga aagtatagtt aatttaatat ataataaaag
    14881 catatcaagt aaagctttaa attaaagtaa ctttatataa aaagtaatat aaattgataa
    14941 aattataaat ttatttatag tgaatatagg ttggatttta tagatacaaa aatagtatat
    15001 atgatatgta gaccattagc atgtttaaag tagcaaaaca tataaaataa aatttgaaag
    15061 gaagtataaa aatgacaaga ccatcaatat atcatagcca aggatacact tgtgcagagg
    15121 cattaataaa gtcttataat gaggaacaca acacagatat accaatctca ataggaagtg
    15181 gtatgggggt tggaatgaat gttggaagtg tttgtggagc tgtaaatgct gctgttgtta
    15241 taatagggta tttaaatggg cgaaatagta atttagatga aaatatagca aggaagtatt
    15301 caagagagct attgaataaa gtaagaaata aatacagctc agaaatatgt gtatctctta
    15361 aaaaaaataa agtaacttgt gaagaaataa taaatttcac atatgatgct ctaaatgata
    15421 tgctaaaaaa tcaaaaataa ttctaagaaa caagtatttt acattcgata gaatacttgt
    15481 ttttttatga
//
LOCUS       DAMJWP010000075        15485 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.079, whole genome shotgun sequence.
ACCESSION   DAMJWP010000075 DAMJWP010000000
VERSION     DAMJWP010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15485
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.079"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(<1..1001)
                     /locus_tag="PC700_001926"
     CDS             complement(<1..1001)
                     /locus_tag="PC700_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HDF2581237.1"
                     /translation="MKRVYIDFEMNMPNSKSKRAVFNSDIIAIGAVMYDEKTKNIDKF
                     KSLIRPVSNEELYPHIQELTHISSEELKSAPSYEEVMRKFKKWLGIFSDIKGIYTFGN
                     LDLTCFNNTDMRSAKKNNHPRFVNNIRDLFVDIKEKYINCGMRCMNYISLKNLLEFVN
                     LEFSGDAHDPLNDAYNLFILDEAITNNVDIQNLLIIRDIIRPPFNDINSNLDNCFNKF
                     KESLYKKEGNYNIVDFSVEIIKTVRMYLLTIIDVNIQNLEIMKDINKKMDTIDKLKDI
                     EEGYFYLLEDVYLDMKDVLEDLMLYRMNEDEYKYEIKNIIKMFDEDLDNEKIYINKC"
     gene            complement(1049..1909)
                     /locus_tag="PC700_001927"
     CDS             complement(1049..1909)
                     /locus_tag="PC700_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HDF2581238.1"
                     /translation="MSELSGKGCEIIVPFEERLPVRDIEKSIIKKYRKNLWSKFMKSI
                     RDYKLVEEGDKIAVAISGGKDSILMAKMFQELKKHGQVNFDVEFIAMDPGYHANIRQL
                     LIDNCEYLNIPIHLFDSRIFEIADEIAKDYPCYMCARMRRGALYSKAEELGCNKLALG
                     HHYDDVIETTMLNLLCAGNFKTMLPKLNSTNFEGIKIIRPLYYIREEHIIRFIQNSGI
                     WPLNCACMVAAKKTGNKRYEIKDLIKSLESNFKNVEKSIFKAAENVNLDSVLGWQKDG
                     EKHSFLENFE"
     gene            2188..2397
                     /locus_tag="PC700_001928"
     CDS             2188..2397
                     /locus_tag="PC700_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HDF2581239.1"
                     /translation="MENKTRDIKKNCGPSLARPYINNQIFTEMYCLSDALKFGTIFPE
                     LNLLESEMYNKELYAKPKKYRGGRR"
     gene            2397..2663
                     /locus_tag="PC700_001929"
     CDS             2397..2663
                     /locus_tag="PC700_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HDF2581240.1"
                     /translation="MKDSSRAELMRKVQETSFACVDMNLYLDNHPDDKNAINTYNSLC
                     NQFAQARYAYENKYGPLTNFGYAPSRCPWQWADQPWPWDREFNY"
     gene            2692..3264
                     /locus_tag="PC700_001930"
     CDS             2692..3264
                     /locus_tag="PC700_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDF2581241.1"
                     /translation="MWIYQKTIQHPVNIKTCDPRMAKFLITQFGGPNGELAASLRYLS
                     QRYTMPTGNMRALLTDIGTEELAHVELICTMVYQLTSDASPEELKAAGLGSNYAQNGY
                     GIYPTDSNGVPFDVRPIAVMSNPVTDLHEDMAAEQKALATYYQLINLTDDVDVIDVLK
                     FLGQREIIHYQRFGEALMDAYELEESQKMF"
     gene            complement(3498..4688)
                     /locus_tag="PC700_001931"
     CDS             complement(3498..4688)
                     /locus_tag="PC700_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDF2581242.1"
                     /translation="MNFNQKRIAASIMATAIIMPTMGNLAYANESEVESVSIESRTIT
                     GNAVNFRKGPGTNHESMGKLYKGDKVEYVGKEGSWVKVKYNGNTGYVHGNYVAINSLG
                     SSNESSDTSVKSTKVVTAKGLNFRTGPSTSSSKISTLGYGTEVGYISESNGWSKISSN
                     GRVGYVSSKYLGTSVNDSTNENVENSSNDLVKGTKVVTAKSLNVRTGPGTSHSKIATL
                     SYGTEVGSISESGGWTKVSYGNQTGYVSSQYLAEKGSVDTSIPSYSTNSPSQGADSVI
                     SFAKTLLGKPYVWGAEGPNSFDCSGFTQYVMKKSAGVSIPRVSRDQSKYGTYVNRGDL
                     RSGDLVFFDTQGSNNGSVSHVGIYIGNGDMIHASSGSSKKVTISNINSSYYSSRYVNA
                     RRVL"
     gene            complement(4948..5379)
                     /gene="dut"
                     /locus_tag="PC700_001932"
     CDS             complement(4948..5379)
                     /gene="dut"
                     /locus_tag="PC700_001932"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDF2581243.1"
                     /translation="MYNLKVKKLNDDAIIPNFAHKGDAGMDLYSIEEVVIPPGETKLI
                     KTGICIELPTMTEAQVRPRSGLALKHSVTVLNTPGTIDEGYRGELKIILINHGKNDFK
                     VEKHMKIAQMIVKPIYDINIEEVKELSDSERGKGGFGSTGF"
     gene            5492..7720
                     /locus_tag="PC700_001933"
     CDS             5492..7720
                     /locus_tag="PC700_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HDF2581244.1"
                     /translation="MKLVLAEKPSVAKTIASFLGAKTRQDGYFEGNDYIITYAVGHLV
                     SLYDMKDYDKDKYSGSWKMNNFPFVPEDKFKFKIDSSKTKQFNTVKKLLNRKDVEYVI
                     NATDNDREGELIAFLIFLLAKNKKPVKRILVNEWTPEDITRGIKNLKDEYEMRNLQAA
                     GYTRLITDWLIGINFTSVATLKYGNGKLLNIGRVILPTVKLVYDRDMEILNFVPKTYY
                     EIEGHFKAEAGEYKGKYVKGKESKFDTLEDANKIIASITSETGKILDKKVTMSKEYAP
                     KLFSLTSLQGYITSKYSNFTSDKVLNVCQSLYEGNGKGGYITYPRTDSIFLEESLASK
                     ASQTLDKLKVGLEYENKIKFSKTKRVFDSSKVDSHSAIIPTYIIPKSLSKDEQLVYDA
                     IKDRFVANFMPPAEYENTEIKTEVDNCTFLTKGKVLKSKGYLEVYNKEEKNDLLPLVN
                     KDDVVDVLEIKPLTKQTTPPKPYTEDTLLKAMKNCGKNVPEEDTTVLSGYSIGTSATR
                     ADVLKKISQVGYVKKKGKSYSITDLGKNLVEIFPVKDLFDVDYTGKLEKSLSDIQKGK
                     YTRKEYLTNIISFIYQNVNIIKQDASKNINTEAYTFDSKTKKFTKTKEKKQKKDVTNT
                     EKEVAISKDSNNKKEVKNGDENQSLGKCPICQGDVLEFDKGFACKNHKECKFVIWKND
                     KYLSLYKKKVNKTMVKNILKKGETKVKSLTAKNGNKFDATLKYDKNPDTGYFNWKIEF
                     DN"
     gene            complement(7831..8316)
                     /locus_tag="PC700_001934"
     CDS             complement(7831..8316)
                     /locus_tag="PC700_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2581245.1"
                     /translation="MDNNNILKIADSFLDFLFVLENNIFKESDLLKKFQNNSDIMKAY
                     FEECPMAPSHAKVILYLMTSNSSSISQIASNLGILKSNMTPIIDRLVEHGLVNKFPDP
                     KDRRILRVELTDKAFKLFDAVKAILKESLVKKLSNLSEEELTLLDEHTLKLSEIVKKL
                     G"
     gene            8568..9791
                     /locus_tag="PC700_001935"
     CDS             8568..9791
                     /locus_tag="PC700_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDF2581246.1"
                     /translation="MKRYLIVFLMCSSLFLVGCGKTKESEPEKPIAVSVQKAVGGEIE
                     NTNSFSGTTKVKDETAVTAQTVGTVQEVYVKLGQNVRKGDELLSISSPELENSVKQSK
                     ASLDLAKASYSSATGGSLEAQVNQAKTALDNAKIQYEESQRNYDNNKILYEQEVISLD
                     QFKKIEFSLEQTKQQLDSAQRAYDTATSKSIPQAKALAKKQLDQAQVSYNLAMSNLDK
                     LTLTSPVDGTITAKNFDSKEMITQSQPAFIISNPNILEVDLNVAESDIGKFKKDGNVD
                     VIIEDQRILGKIDYVPSVVDPQTSLYPVKVLVNNANNKFKAGMSAQVNLSIEKENGAV
                     TVPKKAIFEENGKKYVYIATKDNIAKKHLVQTGIVTEDKIEIKSGVSDKDTVIIGGIS
                     LISDGTKIFPVEKEK"
     gene            9795..12929
                     /locus_tag="PC700_001936"
     CDS             9795..12929
                     /locus_tag="PC700_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HDF2581247.1"
                     /translation="MNLTQTSVKRPLTIIMVFLVLIVFGGIGYKKMSINLMPDIEIPV
                     VMVMTTWTGAGPQDVDEQVSQKVDESLSAVSNVKSTISSSQESVSMVVAQFEFGTNLD
                     EIMNDVRSKVDALQTSLPDDAAKPTVLKLDMNAQAIGQLVISGGNENSSQALRKYAED
                     VIQPKIESIDGVTSADLKGGEKAQVNVIADPAVLSNYGVSLSTIKGVLSSSNKTFPYG
                     SITQGEDKIVLRAIDKLESLDDIKQIQIPVKGGNTVRLDNLCSVDYGYADKDSIYRYN
                     GKDSLVIDISKQQDANTVKVMQSAYKYVDELNKENPQFKIKIANDTSSYISESINSVM
                     SNLLISAVIAFIVIFAFLKSVRASLVVAVAIPISIIGAIAILYFTGESLNLITASSLV
                     ISVGMVVDNATVVIENIFKYRKSGRLSLDDCAIEGTRTVTNAILASTLTTIAIFLPIM
                     FTEGIAKITFGALGKTLIASLAFSFIGAITLVPSVFAKLSRGKNSQKMQEKDSPIFDK
                     VSEAYKKLLSVCLRHQRLVVISSLLIFVASLFGMGFIGMDFMPAADKGELSISIELPK
                     GLSLESNDYYVSMTEKKVSDIPEIKTLITTLTSDSNNSKASIAIELESQKNRKRTTDE
                     IEKEIIDRVATVPDCKINVSQNSSVMGGAGGADFTLEMKGPDLDVLKILGKEAEKEFS
                     SIKGFRNVETSLTDTTQEAQFTIDKHKAQEYGVNTAEIAGMLRTAINGDSVTTATIDD
                     YKVDVNLKFKADTIDSLEDIKQLKVNSATGKEVPLGAFSDIKMADGLQSISRTDGDFS
                     VNITATLDNLDAGTATKLALEKTNKMVIPKDYSIGVGGETEMMNESMASLGFSMVIAI
                     ALVYMVMVAQFESFSKPFIIMFCVPFAFVGVVLSLLVSGQSLSTVGMLGIILLIGIVV
                     NNGIVLIDYIEQLRKTNRNNDLIGLVSIGCAARLRPVLMTTATTILGMLPTALSLGEG
                     GETMQPLAIVIMGGLTVSTLITLVLIPTIYLIFDKWENKFSDKMPKIFGKITDKIGSI
                     KRKIIKKPKLKAKV"
     gene            12963..14261
                     /locus_tag="PC700_001937"
     CDS             12963..14261
                     /locus_tag="PC700_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HDF2581248.1"
                     /translation="MSKIVAIGCGVGIITANLIPVYADNNTSSINNVDNSILSDSNKR
                     TSEKEILTAREAVSAALKNSEKLKMKSEEIKMLKEKLEVQDEFDSFTGSDNSFPYDQI
                     GLLKNQSEQAKGFMEDQIANDITNKFNDLVSRENELDKIKNNLEIKTKEIKDMKLKKD
                     LGLVTSLETESAELELQTLQNTQKAKLQELKNNQDYFKLLTNIDLNNYQLDKEYRFES
                     FRVGGSVDSYMEGKVNEYLKYDQLILERTEESFNDKDENKADLPDRPNLTRPVAPTKP
                     EQGNLTDDEYKVLMDKYEKDYKRYEKEVEAYNLERQTYAAGLTTYANYLQQKFNTENG
                     LVTLEDSKKALKKGLIDSYAQLLALEDTIQITKKQLDLSEKQLKNTKLRYDLGLITLT
                     DYKKQVVSNQDAKNSYDTLIVNYNSLKNGIEKPWILNTGK"
     gene            complement(14471..15298)
                     /locus_tag="PC700_001938"
     CDS             complement(14471..15298)
                     /locus_tag="PC700_001938"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDF2581249.1"
                     /translation="MSRKYSISSMCKLIGVSRSGYYKWLSYSKKSSDRGIKDRIIKDY
                     IIEIHKKYRGTYGRKRICTYLNKILDSPINHKKVYRLMKELGIKSIIRKKVYRRKFKS
                     YEVYDNILNREFRANQPLEKICMDITYIPIGKKFLYMNVAKDLFNGEIVAYEISTKMD
                     TKLVNKTVNQLINMNLAKDCILHTDQGSQYTSRSYSKRLKDNGIIQSMSRRGNCWDNA
                     PIESFFSHFKSELIYLIDTTDPKEMISLINDYIYFYNNERIQLKNGMSSIEYRTHSA"
BASE COUNT         5662 a         2278 c         2225 g         5320 t
ORIGIN      
        1 ttacatttat ttatatatat tttttcatta tctaagtctt catcaaacat ttttattata
       61 ttttttatct catacttgta ctcatcttca ttcattctat aaagcattaa atcttctaat
      121 acatctttca tgtcgaggta cacatcttct aataaataaa aatagccttc ctctatatct
      181 ttcaatttat ctatagtatc cattttttta tttatatctt tcataatttc taaattttgt
      241 atatttacat ctatgattgt taataagtac attctcacag tttttattat ttcaacagaa
      301 aaatctacaa tattataatt tccttctttt ttatataatg actccttaaa tttgttaaaa
      361 caattatcta aattagaatt tatatcgtta aatggtggtc ttattatgtc acgtattatt
      421 aaaagatttt gtatatctac attatttgtg attgcctcat ccaaaataaa taaattatat
      481 gcatcattta atgggtcatg ggcatctcca ctaaactcta agttaacaaa ttcaagtaaa
      541 tttttcaatg atatatagtt catacatctc ataccacaat ttatatattt ttcttttata
      601 tctacaaata aatctctaat attatttaca aaccttggat ggttattttt tttagcactt
      661 ctcatatctg tgttattgaa acatgtcaaa tccaaattac caaaagtata tattccttta
      721 atatcagaaa atatgccaag ccatttttta aattttctca tgacctcttc ataacttggt
      781 gcactcttca attcttcaga agatatatgt gttaattctt gtatatgagg atataactcc
      841 tcattactta ctggtcttat taaagattta aatttatcaa tattttttgt tttttcatca
      901 tacataactg ctccgattgc aattatatca gaattaaata ctgctctttt tgatttagaa
      961 tttggcatat tcatttcaaa atctatatat actcttttca atataaacac cctttccaat
     1021 tgtttatagt gattcttttt aactattttt attcaaaatt ttctaagaaa gaatgtttct
     1081 ccccatcttt ttgccaacca agtactgaat caagatttac attttctgct gccttaaata
     1141 tagatttttc tacattttta aaatttgatt ctaagctctt tattaagtct tttatctcat
     1201 atcttttatt acctgttttt ttagcagcaa ccatacatgc acaatttaat ggccatatac
     1261 cactattttg aataaatctt ataatatgtt cttctcttat ataataaagt ggtcttataa
     1321 tttttatacc ttcaaaatta gtagaattaa gtttaggaag cattgtttta aaatttccag
     1381 cacacaataa gttaagcatt gttgtctcta ttacatcatc ataatgatga cctaatgcaa
     1441 gtttgttaca ccctaattct tctgcctttg aatataatgc tcctctcctc attcttgcac
     1501 acatatagca tggataatct tttgctattt catctgctat ctcaaatatt cttgaatcaa
     1561 ataaatgtat tggtatattt agatactcac aattatctat aagtaattgt cttatatttg
     1621 catggtatcc agggtccata gcaataaatt caacatcaaa attaacttgt ccatgttttt
     1681 ttaactcttg aaacatttta gccataagaa tactgtcttt tccaccagaa atcgctacag
     1741 ctatcttatc cccttcttca accaatttat aatctcttat tgacttcata aacttagacc
     1801 ataaattttt tctatatttc tttattatac ttttttctat atctcttact ggaagtcttt
     1861 cctcaaaagg aactataatt tcacagcctt ttcctgatag ttcactcatc taatcacctc
     1921 gtatatttat gtatatttat gttaataata ttttacaaaa aagtctacct tcatccacaa
     1981 aatactataa tttttacttt aattttacta aataatttat agtttacaat aacacataaa
     2041 cttataataa cataaataac ctctttcttt tattatcttt tttatttttc tcatttaata
     2101 ttttattatt agtaacgatt tttaaatgat tgaatattat acactagata gagttaccta
     2161 tctatttaca acaaggaggt tttcatcttg gaaaataaga ctagagatat caaaaaaaat
     2221 tgtggtccat ctttggctag accatacatt aataatcaaa ttttcacaga aatgtactgc
     2281 ctttctgatg cattaaaatt tggaactata tttccagaac taaacttact agagtctgaa
     2341 atgtacaaca aagaattata tgctaagcca aaaaaatata gaggaggtag aagataatga
     2401 aagactcatc tagagctgaa cttatgagaa aagttcagga aacatctttt gcatgtgttg
     2461 atatgaactt atatttagac aatcaccctg atgataaaaa tgctatcaac acttataatt
     2521 cattatgtaa tcaatttgcg caagctagat atgcttacga aaataaatac ggtcctttaa
     2581 ctaactttgg gtatgctcca agtagatgcc cttggcaatg ggctgaccaa ccttggcctt
     2641 gggacagaga atttaattat tagacatcaa gtattggagg attgaaaatt catgtggata
     2701 tatcagaaaa ctatacaaca cccagttaat ataaaaactt gtgaccctag aatggctaaa
     2761 tttcttataa ctcaatttgg tgggccaaat ggagaacttg ctgcatcttt aagatattta
     2821 agccaaagat atacaatgcc tactggtaat atgcgtgcac ttttaacaga tattggtaca
     2881 gaagagctag ctcacgttga gcttatatgt actatggttt atcaattaac ttctgatgca
     2941 agcccagaag agttaaaagc tgcaggtctt ggttcaaact atgctcaaaa tggatatgga
     3001 atttatccaa cagattcaaa tggtgttcca tttgatgtaa gacctatagc agttatgtca
     3061 aatcccgtaa ccgacttaca tgaggatatg gcagctgaac aaaaagcact tgcaacttat
     3121 tatcaactta taaacctaac agatgacgtt gatgttatag atgtattaaa attcttaggt
     3181 caaagagaaa taattcacta tcaaagattt ggtgaagctt taatggatgc ttacgagtta
     3241 gaagaatctc aaaaaatgtt ctaaaatttt tatttgtata actattatta tttgataatt
     3301 tataatttac tgttgtgtta aaatttataa aaaatataaa tataatttat tttctataaa
     3361 cagagaaaat aaataaaaag caggtatcaa tcaaatagta acatgtatta tactatacat
     3421 tactatcaat attgtacctg ctttcatatt ataagcaata aagaaaaatc tatttattag
     3481 gttttataat aaaacaatta taaaactctt cttgcattta catatctact tgaatagtag
     3541 ctactattta tattagatat tgttactttt tttgatgaac ctgaagatgc atgtatcata
     3601 tctccatttc ctatgtatat tccaacatga cttacagaac cattatttga accttgtgta
     3661 tcaaagaata ctaaatctcc acttctaagg tctcctctat ttacatatgt tccatactta
     3721 ctttggtctc tagatactct tggtatacta actcctgcag attttttcat aacgtactgt
     3781 gtgaatccag aacagtcaaa actatttgga ccttctgctc cccatacata tggttttcca
     3841 agtagtgtct ttgcaaaact tattacacta tcagctcctt gacttggaga attagtcgaa
     3901 taagatggaa ttgaagtatc aacagaacct ttttcagcaa ggtattgact tgacacatat
     3961 ccagtttgat tgccataact tacttttgtc catcctccac tttcagaaat acttccaacc
     4021 tcagtaccat aacttaatgt agctatttta gaatgacttg tacctggtcc tgttctaacg
     4081 ttcaatgact ttgctgttac aacctttgta ccctttacaa ggtcatttga actgttttca
     4141 acattttcat ttgtagagtc atttacacta gttcctaaat atttacttga aacataacct
     4201 actctaccat tagaacttat ttttgaccat ccatttgact cagatatgta tccgacttca
     4261 gtcccatatc ctagtgttga tattttagaa ctacttgtac ttggtcctgt tctaaaattt
     4321 aatccttttg ctgttacaac ttttgtcgac ttaactgaag tatcactact ttcattgcta
     4381 cttccaaggc tatttattgc tacataattt ccatgcacat aacctgtgtt tccattatat
     4441 ttaactttta cccaagaacc ttcttttcct acgtactcaa ctttatcacc tttgtaaagt
     4501 ttccccattg attcatggtt tgtacctggt ccttttctaa aatttaccgc attccctgtt
     4561 attgttctgc tttctatact cacactctct acttcagact cattagcata tgctaaattc
     4621 cccatagtag gcattattat cgcagttgcc attatactgg ctgctattct cttttgatta
     4681 aaattcataa ctttccactc atctcctctt ttctactatc tttatttcac ttcaaaatat
     4741 aaaagaaata attgacacta ttaaaatatc aattatttct tttatattaa tattattaca
     4801 aatttgtaac tatttgtcta tagtaatatt tgcttaccat aaattaccat gtataaattt
     4861 taatcttcaa tagcctgtaa tttagtaata tagccatctt ttaccttttt taaaaatggc
     4921 tatatttttt aacttttttg taacttttta aaatccagtt gagccaaacc caccttttcc
     4981 tctttcggag tcacttaatt cttttacttc ttctatgtta atatcataga taggttttac
     5041 tatcatttga gcaattttca tatgcttttc aaccttaaaa tcatttttcc catgatttat
     5101 taatataatt ttaagctctc ctctgtatcc ttcgtcaata gttcctggtg tatttaatac
     5161 agtaacagaa tgctttaagg caagcccact tctaggtctt acctgcgctt ctgtcatagt
     5221 aggaagctct atacatatcc cagttttgat aagtttagtc tctccaggag gtattacaac
     5281 ctcttctata gaatacaaat ccataccagc atcacctttg tgagcaaaat taggtattat
     5341 agcatcatca tttaattttt ttacttttaa attgtacatc ttatatctcc tttcaacttt
     5401 ctataaatat tataacttaa atttcgacct cttgaagttt tttatttttt tgtggtaata
     5461 taattattag tgttttaaag gaggattttt tatgaaactt gtacttgctg agaaaccatc
     5521 tgtggcaaaa actatagcat catttttagg tgcaaagacc agacaagatg gttactttga
     5581 aggaaatgat tatataatta cttatgcagt tggacactta gtatcattat atgatatgaa
     5641 ggattatgat aaagataaat actctggctc ttggaaaatg aacaatttcc catttgttcc
     5701 tgaggataag tttaaattta agattgatag ttctaaaact aaacaattca acactgtaaa
     5761 aaagcttttg aatagaaaag atgtagaata tgtaataaat gccactgaca atgaccgaga
     5821 aggagaactt atagcatttt taatattttt actggcaaaa aataaaaaac cagtaaaaag
     5881 aatcttggta aatgaatgga caccagaaga tattacaaga ggtataaaaa acctaaaaga
     5941 tgaatatgaa atgagaaatc ttcaagctgc tggatatact aggcttatta ctgactggtt
     6001 gattggaata aatttcactt ctgttgctac attgaaatat ggaaatggga aactattaaa
     6061 cataggtagg gtaattcttc caacagttaa attagtctat gatagagata tggaaatact
     6121 aaactttgtt cctaaaactt attatgaaat agaaggacat tttaaagctg aagctggtga
     6181 atataaaggt aaatatgtta agggtaaaga aagtaaattt gatacacttg aagatgcaaa
     6241 taaaattata gcttcaatta catctgaaac tggtaaaata ctagataaaa aagttactat
     6301 gtcaaaggaa tatgcaccaa aactttttag tttgacatca cttcaaggat atataacttc
     6361 taagtattcc aattttacat ctgataaggt tttaaatgtc tgtcaatctc tctatgaggg
     6421 taatggtaag ggtggataca taacttaccc tagaactgat tccattttct tagaagaaag
     6481 tttggcttca aaagcttctc aaactttaga taaattaaag gttggtcttg aatatgaaaa
     6541 taaaattaaa ttttctaaaa ctaaaagagt ttttgattct tctaaagtag actcccatag
     6601 tgctataatt ccaacttata taattccaaa aagtttatct aaggatgaac aattagttta
     6661 tgatgccata aaagatagat ttgtagcaaa ctttatgcct cctgctgaat atgagaatac
     6721 agaaattaag actgaagtag ataattgtac tttcttaaca aaaggtaaag tgcttaaatc
     6781 aaaaggatac cttgaggtat ataataagga agaaaaaaac gatttacttc cacttgtaaa
     6841 taaagatgat gttgtagatg tgttagaaat aaaaccatta actaagcaaa ctactccacc
     6901 taaaccatat actgaggaca cccttttaaa ggctatgaaa aattgtggta aaaatgtgcc
     6961 tgaagaagat acaacagtgc tatctggata ttcgattgga acatcagcca cacgtgctga
     7021 tgtgttaaag aagataagtc aagtgggcta tgtaaagaaa aaagggaaat cttattctat
     7081 aacagacctt gggaaaaatt tagttgaaat ttttcctgtt aaagacttat ttgatgtaga
     7141 ttatactgga aaacttgaaa aaagtttaag cgatattcaa aaaggaaagt atacaagaaa
     7201 agaatatctt actaatatca ttagttttat atatcaaaat gtcaatatta taaaacaaga
     7261 tgcttcaaaa aatataaaca cagaagcata tactttcgat tcaaaaacta aaaaatttac
     7321 taaaacaaaa gaaaaaaaac agaaaaaaga tgtaactaat actgaaaaag aagttgctat
     7381 atcaaaagat tctaataaca aaaaagaagt taaaaatggt gatgaaaatc aatctcttgg
     7441 caaatgcccc atttgtcaag gtgatgtact agaatttgac aaaggctttg cttgtaaaaa
     7501 tcataaagaa tgtaaatttg ttatttggaa aaatgataag tatttaagtc tttataaaaa
     7561 gaaagttaac aaaacaatgg tcaaaaatat tttaaaaaaa ggtgaaacta aagtaaaatc
     7621 tttaactgct aaaaacggta ataaatttga tgctacatta aaatatgata aaaatccaga
     7681 taccggctat ttcaattgga aaatagagtt tgacaactaa atattcataa gaaaagaaat
     7741 agcagtagta taataatcta tattatatta ctgctatttc ttttcttatg aagttttaac
     7801 ttttttaata gtgactttta caatcacact ctaacctaat ttctttacta tttctgataa
     7861 ctttaatgta tgttcatcta ataaagttag ttcctcttca gaaagattag ataatttttt
     7921 tactagagat tctttgagaa tagcttttac tgcatcaaat aatttaaatg ctttatctgt
     7981 taattcaacc ctaagaatcc ttctatcttt tgggtcagga aatttattga ctaaaccatg
     8041 ttccactaat ctgtcaataa tcggagtcat atttgatttt aaaattccta aattagaagc
     8101 tatttgagaa attgatgatg aatttgatgt cataagataa agaattacct tagcatgtga
     8161 tggagccatt ggacattctt caaaataagc tttcattata tcagagttat tttgaaactt
     8221 ttttaacaaa tcgctttctt taaatatatt attttcaagc acaaataaaa aatctaaaaa
     8281 agagtccgct attttaagta tattgttgtt atccatattt gtaacatccc ttcaatagat
     8341 aatataataa aacattgttg tttaatatag tataaattat aaactattaa taaatttaaa
     8401 acaagaaaaa gttattgact cataataatt ataaatttat aatcaaatta gagaattata
     8461 aattgccaat aaagggaagt tttttaatta gtctaaaact aaacttaaat tataagacac
     8521 tttaaattct acaacaagct agtgtgcaat atataatagg aggagatatg aaaagatatt
     8581 taatagtttt cttaatgtgt agttcactgt ttttagttgg atgtggaaaa acaaaagaat
     8641 ctgaacctga gaaaccaata gcagtttctg ttcaaaaagc agtaggtgga gaaattgaaa
     8701 acacaaattc tttctcagga actacaaaag ttaaagatga aacagctgtt actgcacaga
     8761 ctgttggcac agttcaagaa gtgtatgtaa aacttgggca aaatgtaaga aaaggagatg
     8821 aattattgag tataagcagt ccagagctag aaaatagtgt aaagcaaagt aaagcctcac
     8881 tagatttagc aaaagcaagt tattcaagtg ccactggtgg ttcattagag gcacaagtaa
     8941 atcaggcaaa aacagctctt gacaatgcaa agattcaata tgaggagtct caaagaaatt
     9001 atgacaataa taagattctt tatgaacagg aagtcattag tttagaccaa tttaaaaaaa
     9061 tagagttttc tttagagcaa acaaaacaac agttagattc tgctcaaaga gcatacgata
     9121 cagcaacttc aaaaagtata cctcaagcta aagcacttgc taaaaaacag cttgaccaag
     9181 ctcaagtatc atataattta gcaatgagta atttagacaa attaacatta acatctccag
     9241 tagatggtac aataacagct aaaaactttg attcaaagga aatgataaca caatcacaac
     9301 cagcttttat tatttctaat cctaatatac tagaagttga tttaaatgta gcagaatcag
     9361 atataggtaa atttaagaaa gatggaaatg tagatgttat aattgaagac caaaggattt
     9421 taggaaaaat agattatgta ccttcagtag ttgaccctca gacatcttta tatcctgtta
     9481 aagtacttgt aaataatgca aacaacaaat ttaaggcagg tatgtctgca caagtaaact
     9541 taagtattga aaaagaaaat ggtgctgtta cagtgcctaa aaaagcaata tttgaagaaa
     9601 atggtaaaaa atatgtatac atagcaacaa aagacaatat agcaaagaaa cacttggttc
     9661 aaactggaat tgtaacagag gataaaatag aaattaaaag tggtgtaagt gataaagata
     9721 cagtgattat tggaggaata agtcttattt ctgacggaac taagatattt cctgtagaaa
     9781 aggagaagta gactatgaat ttaacacaga cttccgttaa acgtcctctc acaatcataa
     9841 tggtcttttt agtattaata gtttttggtg gtattggata taaaaaaatg tcaataaatc
     9901 taatgcctga tatagaaata ccagttgtaa tggtcatgac tacttggaca ggtgctggtc
     9961 ctcaagacgt tgatgaacaa gttagtcaaa aagttgatga aagtttatct gccgtttcaa
    10021 atgtaaaatc tactatatca agttcacaag aaagtgtttc tatggtagtt gctcagtttg
    10081 aatttggaac taatttggat gaaataatga atgatgttag gtctaaagtt gatgcattac
    10141 agaccagcct cccagatgat gcagcaaaac caacagtttt aaagctggat atgaatgcac
    10201 aggctatagg tcaacttgtc atatctggag gaaatgaaaa tagttcacaa gccttaagaa
    10261 aatacgctga agatgtaatt cagcctaaaa tagaatccat agatggtgtt acttcagcag
    10321 accttaaagg tggagaaaaa gcacaggtta atgtaatagc tgacccagct gttctttcaa
    10381 attatggagt tagtttatcc actataaaag gtgtcttatc ctctagtaat aaaacattcc
    10441 cttatggttc aattactcaa ggtgaggata aaatagtttt aagagcaatt gataaattag
    10501 aatccctaga tgatataaaa cagattcaaa ttcctgtaaa aggaggaaat acagttagat
    10561 tagataatct ttgtagtgta gattatggat atgcagacaa agatagtatt tatagataca
    10621 atggaaaaga cagtttggta atagatattt ctaaacaaca agatgccaac actgtaaaag
    10681 ttatgcagag tgcatacaaa tacgtagatg aattgaataa ggaaaatcct caatttaaaa
    10741 ttaaaattgc taatgataca agttcataca taagtgaatc aataaacagt gtaatgagca
    10801 acttgcttat aagtgctgtt atcgcattta ttgtaatatt tgctttccta aaaagtgtaa
    10861 gagcttcact agttgttgca gttgcaatac caatttctat aataggtgct atagcaattc
    10921 tctactttac tggtgaaagt ttaaatctta ttactgcaag ttcattggta atttcagttg
    10981 gtatggttgt tgataatgct acagttgtaa ttgaaaatat atttaaatat aggaaaagtg
    11041 gcagattgag tcttgatgat tgtgctattg agggaacaag aactgtaaca aatgcaatac
    11101 ttgcatcaac attgaccacg atagcaatat tcttacctat catgtttaca gaaggtatag
    11161 ctaaaataac ttttggggca ttaggtaaaa cactaatagc ttcactagca ttctccttta
    11221 taggtgcaat tacactcgtt cctagtgtat ttgcaaaatt aagcagagga aaaaatagtc
    11281 aaaagatgca agaaaaagat agtcctatat ttgataaggt aagtgaagca tataaaaaat
    11341 tattaagtgt atgtttaaga catcaacgct tagttgtaat atcaagttta cttatttttg
    11401 tagcttcctt atttggaatg ggatttatag gtatggactt tatgcctgca gcagacaagg
    11461 gtgaattatc tatttctata gaacttccaa aaggtcttag cctagaatcc aatgattact
    11521 atgtatctat gactgagaaa aaagtctcag atattccaga aattaaaact ttaataacaa
    11581 ctttgacatc agattcaaat aatagtaaag caagtatagc aattgaatta gaatctcaaa
    11641 aaaatagaaa aagaactact gatgaaatag aaaaagagat aattgataga gtggctactg
    11701 taccagattg taaaataaat gtaagtcaaa acagtagtgt tatgggtgga gctggaggag
    11761 cagacttcac attagaaatg aaaggtcctg atttagatgt attaaaaata ctgggaaaag
    11821 aagctgaaaa agaatttagc tctataaaag gatttagaaa tgttgaaact tctcttacag
    11881 atacaacaca agaagctcaa tttactatag ataaacataa ggctcaagaa tatggtgtaa
    11941 atactgctga aattgcaggc atgttacgta cagcaataaa tggtgactcc gtaaccactg
    12001 ctactattga tgattacaaa gtggatgtaa acctaaaatt taaagcagat actattgata
    12061 gcttagaaga tataaagcaa ctaaaagtaa actctgcaac tggaaaagaa gttcctcttg
    12121 gtgctttttc agatataaaa atggcagatg gtctacaatc tatatctagg actgatggag
    12181 atttttctgt aaatataact gctacacttg acaatttaga tgctggtact gctacaaaac
    12241 tagcactaga aaaaacgaat aaaatggtaa ttcctaaaga ttactctata ggtgttggtg
    12301 gtgaaacaga aatgatgaat gaatctatgg caagtcttgg attttcaatg gtcatagcta
    12361 tagcattagt ttatatggta atggtagctc agtttgaatc atttagtaaa ccatttataa
    12421 tcatgttctg tgttccattt gcattcgttg gagttgtgct ttcgcttcta gttagtggtc
    12481 aaagtttaag tactgtcggt atgctaggta taatcttact tatcggtatt gttgttaata
    12541 atggtatagt tcttattgac tacatagagc aacttagaaa aacaaatcgt aacaatgatt
    12601 taattggttt ggtttcaatt ggttgtgcag caagattaag accagtctta atgactacag
    12661 caacaacaat tttaggtatg ttgccaacag cactatctct tggtgaaggt ggagaaacta
    12721 tgcaaccact agccatagtt ataatgggtg gtctaactgt atctacactt ataactttgg
    12781 tgcttatacc tactatatac ttaatatttg ataaatggga aaataagttt tcagacaaaa
    12841 tgcctaaaat atttggtaag ataacagata aaataggaag tataaaacgt aaaataataa
    12901 agaaaccaaa attgaaagct aaagtttaaa tagaatggag ggtgattgta ttgaataaaa
    12961 aaatgagcaa aatagttgca attggctgtg gtgttggtat aataactgcc aatttaatcc
    13021 cagtttacgc agataataat acttcttcta taaacaacgt tgataattct attttaagtg
    13081 actcaaataa aagaacatct gaaaaggaaa ttttgactgc tagagaagct gttagtgctg
    13141 cacttaaaaa tagtgaaaaa ttaaaaatga aatcagaaga aataaaaatg ttaaaagaaa
    13201 agttagaagt acaagatgag tttgatagtt ttactggaag tgacaactcg tttccatacg
    13261 accaaattgg acttcttaaa aatcaaagtg aacaggctaa aggctttatg gaggaccaaa
    13321 tagcaaatga tattactaat aaatttaacg atttagtttc aagagaaaat gagctagata
    13381 agataaaaaa caatcttgaa ataaaaacta aagaaattaa agatatgaaa ttaaaaaaag
    13441 atttagggtt agtaacatct ttagaaactg aatcagctga acttgaactt caaacactac
    13501 aaaatactca aaaagctaaa cttcaagaac taaaaaacaa tcaagactat tttaagttat
    13561 tgactaatat agatttaaat aattatcaat tggataaaga atatagattt gaatctttta
    13621 gagtaggtgg ctcagttgat tcatatatgg aaggtaaggt taatgaatat ttaaaatacg
    13681 accaattgat tttagaacgt acagaagaaa gctttaatga taaagatgag aacaaagcag
    13741 atttaccaga tagacctaat cttacaagac cagttgctcc tacaaaacca gagcaaggta
    13801 acctaactga tgatgaatac aaggttctta tggataagta tgaaaaagac tacaagagat
    13861 atgaaaaaga agttgaagca tacaatcttg aaagacagac ttatgctgct ggccttacta
    13921 cttatgcaaa ttatcttcaa caaaaattta ataccgaaaa tggtctagta acattagaag
    13981 acagtaaaaa agctcttaaa aaagggttaa ttgatagcta tgctcaatta cttgctttag
    14041 aagatacaat tcagattact aaaaaacaac ttgatttatc agaaaaacaa ttaaaaaata
    14101 ctaaattaag atatgattta ggattaatta ctctaactga ttataaaaaa caagtggtaa
    14161 gtaatcaaga tgctaaaaat agctatgaca ctcttattgt gaattataac tcactaaaaa
    14221 atggtataga aaaaccttgg atacttaata ctggaaaata attctggtaa atagaaagat
    14281 tactttaaca tacgtttgat taaaaaatag ttatgcacac tcgtggcttt agtcatggaa
    14341 gattcagaag gcaaataata gaaatagatt agtaaaaata tatttactta taaaactgta
    14401 tacttttata gaggctatct caaaattgga tatgtccctg tcaagtagac agacttaaaa
    14461 aaggctatct ttatgcggaa tgagttcgat attcaatcga actcattccg ttttttaatt
    14521 gtattctttc attattataa aaataaatat aatcatttat tagactaatc atttcctttg
    14581 gatctgtagt atcaattaaa tatattaatt cagatttaaa atgactgaaa aaactttcta
    14641 taggagcatt atcccagcag ttgccccttc gggacataga ttgtataata ccattatctt
    14701 taagcctttt agagtaagat ctacttgtat attgtgaacc ttgatctgta tgtaaaatac
    14761 aatctttagc taaattcata tttattagtt gatttactgt tttattaact aatttagtat
    14821 ccatttttgt acttatctca tatgccacta tttcaccatt aaataaatct ttggctacat
    14881 tcatgtataa gaatttttta ccgataggta tgtatgttat atccatacaa attttttcta
    14941 atggttgatt agctctaaat tcacgattta atatattatc atatacctca tatgatttaa
    15001 acttacgtct atatactttc tttctaataa tagatttaat tcctagttct ttcatcaacc
    15061 tatatacttt cttatgatta atagggctat caagtatttt atttaaataa gtacaaattc
    15121 tttttctacc gtaagttcct ctatattttt tatgtatttc aattatataa tccttaataa
    15181 ttctatcttt gataccccta tcggaagatt ttttagaata acttagccat ttatagtaac
    15241 cgcttcttga cacacctatc aacttacaca tactagaaat actatacttc ctagacataa
    15301 gtttaattgt tttaaattta tcgcttactt tgacttgtcc aatagggtac ttaacttttt
    15361 taaaaattca ttctccatgc gtaagtactt caattcttct tcaacagatt taaattttct
    15421 attatcattt tctagactaa tatttttaaa tctgcctcta gtttcctcat taaatgcagt
    15481 tttac
//
LOCUS       DAMJWP010000076        15484 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.080, whole genome shotgun sequence.
ACCESSION   DAMJWP010000076 DAMJWP010000000
VERSION     DAMJWP010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15484
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.080"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            204..761
                     /gene="efp"
                     /locus_tag="PC700_001939"
     CDS             204..761
                     /gene="efp"
                     /locus_tag="PC700_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDF2581250.1"
                     /translation="MVSAGDFRKGVTFEKDGQPCLVVDFQHVKPGKGAAFVRTKYKNL
                     KTGAIREESFNPSEKFPKAVIDTRQMQYLYNDGELYYFMDQENFEQIPLNYEQVEDAI
                     KFLKENEVATIRFYQGQPFQVEAPNFAELEVTDTEPGIKGDTASNVTKAATVETGAVV
                     QVPLFINTGDKVKIDTRTGEYLSRV"
     gene            853..1308
                     /locus_tag="PC700_001940"
     CDS             853..1308
                     /locus_tag="PC700_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2581251.1"
                     /translation="MKHLYEEVAYLKGLAEGLEISAESKEGKMIHKIVDALEVFADAI
                     VTLDEEQEELQDFVESIDEDLADLEEDIADMEEDLYEEDDDEDDEDFSYIEMECPNCG
                     ELVEIDEDLLYDDEVDVVCPDCKAVILSSEDDCDDDCTCGGCPSCDDRE"
     gene            1471..2181
                     /locus_tag="PC700_001941"
     CDS             1471..2181
                     /locus_tag="PC700_001941"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDF2581252.1"
                     /translation="MKISKKLLDNIQRFENVINYKFKNKEYILEALTHSSYSNENKKY
                     NFNERLEFLGDSVLGIVISDYLFNEEANLPEGELTKLRANIVCEDSLSEVANDINLGI
                     HMLLGRGEEATGGRHRTSILADAFEAVIAAIYLDGGFESARQFILHHMENIIYDSRKG
                     NIFRDYKTHLQEVLQGNGENNIWYRLIEEKGPDHNKRFVMEVGINDDVLGIGEGKSKK
                     EAEQLAAKIALKKKLWEK"
     gene            2235..3305
                     /locus_tag="PC700_001942"
     CDS             2235..3305
                     /locus_tag="PC700_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2581253.1"
                     /translation="MKKRIIPIFVPHKGCPHDCIFCNQKKITGVSTDVTSEDARNIIE
                     ECLETIDKDADVEIAFFGGSFTAIDIDIQKSLLSVAKEYVEKGLVKDIRMSTRPDCID
                     EDILSMLKEYKTSIIELGVQSLDEKVLLDSIRGHQSEIVYKSSKMIKNSGIKLGLQMM
                     IGLPADTEEKCIFTAKKFVELKPDCVRVYPTLVVKDTGLEKLMEQNKYNPFTLEESVN
                     IVKKVLVLFYVNNINVIRVGLQATDDIQIGKAVLAGPYHPAFRELVEADMIKDYLEFV
                     ILQNKNIKQMLVKSNKKNISKIIGNKKTNVKYMEEKFGVLLKTQESDLDINQLEIVLD
                     GKSLISANMRDIHRKLYDIYDL"
     gene            3333..6887
                     /gene="smc"
                     /locus_tag="PC700_001943"
     CDS             3333..6887
                     /gene="smc"
                     /locus_tag="PC700_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HDF2581254.1"
                     /translation="MYLKRLELKGFKSFPVKTDIIFKEGITAIVGPNGSGKSNISDAV
                     RWVLGEQSIKSLRGDKLEDVIFAGTDTKKPMNYCEVALTIDNSENQLELDFTEVTIRR
                     RAYRNGESEFFLNNKSCRLKDIKEVFLDTGIGKDGYSIIEQGKVDEILSNNPLSRRKV
                     FDEACGISKYRYKKQEAERNLSNTKENLERIDDVYIEIENQLKPLFNQQTKAKKYLEI
                     SEKLKILEVNSFIREIEGIEKELSEVNEHRKVIEKELNEKEEQKNVVEKKQEDINKEV
                     EVLQDVIEKSVDYINSIKGVISKKESQINLIKERIRNFTNEISRKNLEINDIKEKLNE
                     NKQYIKELESNKLSGSEELSTLQENIKVLEASKDKQKIKLESLNNEIELLKESIIDIL
                     NKKQEFSNKLSTLNANKENMNIRDENINSEITELNKNIEIKSSELDTINKEFNMQNEN
                     LKNVNNRHKELSINLQDSISEHNKLEDEIQKSKYNLNGYNSKLNVYIDMENHYEGFNR
                     GVKEVLKNKNLKGVHGALGQIINVPEKYEKSIEAALGAYMQNIITDNEFSAKSAINYL
                     KQNNLGRVTFLPLNIIKSNKVSLGNLKANTKFIGIASDLITFDEKYRNIIENILGRTI
                     LINNIDEGIKFAKETGHRFKIVTLDGEILNPGGSLTGGSLKTNGNILSRKRYINEYTE
                     KISNIKNEISHLELKRESLDKDVKNIKNEIDSHESKIKDLEKSIIIKSTSIKNVESEI
                     ESLKGSITKLENEKNDLNSNLNYTLEKSDDVRKDMEELDDLYNKNKEKIDALNEEIKR
                     YNDLYDKEKSEFDELNLSLVKKTEAYNSIVRDIKRISGENCELEEKNKQLEESLNYEE
                     HEIIKLQDSIATEEKEKENLTKQLGDSNRNLETRKIAKDDLKNSFDEINKELKTIDRQ
                     HIELKESLFKVGGRLERLKTSQDTYINKLFEQYDMTLVQALEIKDEDLDIDRKFLESL
                     KREIRSLGNINIGSIKEYEEIKERYDFYSEQKQDLEESMEEIEKLIHTLEENMKSEFE
                     IKFEEISKNFKYVYKRLFGGGCGELTILDKENLLESDILITAQPPGKKMKNLNLLSGG
                     EKALTAISILFAILITKPTPFCILDEIEAPLDDANIFRFGEFLKDLSKETQFISVTHR
                     RGTMEAADYIYGVTMQEKAISKVISLKLKEAQEITDII"
     gene            6914..8194
                     /gene="ftsY"
                     /locus_tag="PC700_001944"
     CDS             6914..8194
                     /gene="ftsY"
                     /locus_tag="PC700_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDF2581255.1"
                     /translation="MLKKLFGFGKDKEKEIEKKDAEEEIEVEDSVDSLENLEETIFSG
                     LEEEVIDKVEDIEEKTEESVNNEGTEEIENFEKIEVEMDSNAKEVENISNNDSQEEII
                     EELENYDEIESKEIEDKEDKKVNLFERLKQGLTKAKQGITDRIDEVLKSYTKIDEELL
                     EDLEEILITADVGVNTTMDIIERLRDKIKQKGITEPIKVREELKSIVEDILTNENSTL
                     DIEPAPCIILMVGVNGVGKTTTIGKLANRYKKDGKKVLLAAADTFRAAATEQLEIWAN
                     RTNVDIIKHQEGADPGAVVFDAIKAAKARKTDVLICDTAGRLHNKANLMNELGKVFKI
                     VDREFPEAKREVLLVVDATTGQNAVVQAKTFKEVADITGIVLTKLDGTAKGGVVLAVK
                     SEVDVPVKLIGVGESVEDLQDFNAKSFSDALFGN"
     gene            8297..8665
                     /locus_tag="PC700_001945"
     CDS             8297..8665
                     /locus_tag="PC700_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HDF2581256.1"
                     /translation="MNIEKMVEIGLLFEQYKELLTDKQKEIVALYYEEDYSLGEISEN
                     LNVSRQGVYDTLKRSEKILRDYEEKLHLVSKIQEQEKNIKIIKDKIIDIKEDLLHNRD
                     CANLIPKLENIEDVCREMIK"
     gene            8662..10014
                     /gene="ffh"
                     /locus_tag="PC700_001946"
     CDS             8662..10014
                     /gene="ffh"
                     /locus_tag="PC700_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDF2581257.1"
                     /translation="MIFEGLSDKLQGAFSKLKSKGKLTEVDVKNAMREVKLALLEADV
                     NFKVVKDFIKKVQERCVGQEVMQSLTPAQHVIKIVNEELTSLMGDVQSKVMISSKPPT
                     ILMMVGLQGAGKTTTAGKLGGYFKKQGKKPLLVACDIYRPAAIKQLQVVGEKLDIPVF
                     NMGDKENPVNIAKAGLSHAIKNANDLVIIDTAGRLHIDEVLMAELKSIKSEVKPHEIL
                     LVVDSMTGQDAVNVAESFNEALGVDGVVLTKLDGDTRGGAALSIRAVTQKPIKFMGMG
                     EKLDDIEPFHPDRMASRILGMGDVLSLIEKAQENIDLEKAKELEQKIKKQELDFEDFL
                     QQMEQIQNMGPLDKILGMIPGMGNVKDQLGDVDLNGKEMKRTKAIIQSMTTYERRNPG
                     VLNASRKKRIAKGSGTSVQDVNRLIKQLNEMKKMMKMFAGSQKSMKKRGGLGGLPFFK
                     "
     gene            10052..10324
                     /gene="rpsP"
                     /locus_tag="PC700_001947"
     CDS             10052..10324
                     /gene="rpsP"
                     /locus_tag="PC700_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDF2581258.1"
                     /translation="MAVKIRLKRMGANKKPFYRIVVADSRAPRDGKFIEEIGYYNPIS
                     EPKQVRINDEKAIKWLATGAQPTEVVKKLLVKNGVIEKFEASKQAK"
     gene            10389..10616
                     /locus_tag="PC700_001948"
     CDS             10389..10616
                     /locus_tag="PC700_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HDF2581259.1"
                     /translation="MKELVVDIAKALVDNPDSVVVEEFEDNDGIVLKLTVAQEDMGKV
                     IGKQGRIAKAIRTVVRSVANRENIKVSLEIV"
     gene            10724..11239
                     /gene="rimM"
                     /locus_tag="PC700_001949"
     CDS             10724..11239
                     /gene="rimM"
                     /locus_tag="PC700_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HDF2581260.1"
                     /translation="MKEKLTHFKVGQIVNTQGLKGEVRVYPLTDDIERFDELKDFYLG
                     KDLNNKLAVEKVRYKGSMVIMKIKGIDSIEKAEKLKNKFMYVSREDSRELEEGEFFIA
                     DMIGMEVLTVDGKYVGILEDVLQYSANDVYVVKGEEDKEFMIPAIKKFVPTIDIDERK
                     MIIDPIKGMID"
     gene            11248..11943
                     /gene="trmD"
                     /locus_tag="PC700_001950"
     CDS             11248..11943
                     /gene="trmD"
                     /locus_tag="PC700_001950"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDF2581261.1"
                     /translation="MRFHIMTLFPEIFNSYMDESIMKRAVEKGIIEVYIYNIRDFSNN
                     KHKKVDDYPFGGGVGMVMTPQPIYDTYKHIITTHNISNPSVIYLTPKGKVYNQSIAKQ
                     MSLKEDIILLCGHYEGVDERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGV
                     LNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITK
                     ERRFDLYKKSNEK"
     gene            12128..12478
                     /gene="rplS"
                     /locus_tag="PC700_001951"
     CDS             12128..12478
                     /gene="rplS"
                     /locus_tag="PC700_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDF2581262.1"
                     /translation="MNEMLRAIEQEQLKNEVPNFGPGDTVKVHVRIIEGKRERIQVFE
                     GVVLKRQGGGARETFTVRKMSFNVGVERTFPVHSPKIEKIEVTRKGKVRRAKLNYLRG
                     RVGKAAKIKEARNK"
     gene            12799..13695
                     /gene="ylqF"
                     /locus_tag="PC700_001952"
     CDS             12799..13695
                     /gene="ylqF"
                     /locus_tag="PC700_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HDF2581263.1"
                     /translation="MSNEYEEYLMDDNLHINWYPGHMKKTKELVKNNLKLIDVVIELL
                     DARIPFSSKNPDIDRLVGDKPRVVVLNKSDMADRDKLNQWIEYYKKINIKAIPVDTIK
                     GVGINNIIEECKNVTREKMSSLKDKGRKERAIRIMIVGVPNVGKSSLINKLTGRKSTQ
                     TGDKPGVTKGKQWVRLKGNLELLDTPGILWPKFEDQEVALNLAFSRAIKDEILDTETL
                     ALRLIEKLMKIEPEKLKARYKLDCLGETPIETMDMIGHKRGFITGKKELDYTRIAATV
                     LNEFRDGKIGNITLEVPENVKR"
     gene            14084..15376
                     /gene="brnQ"
                     /locus_tag="PC700_001953"
     CDS             14084..15376
                     /gene="brnQ"
                     /locus_tag="PC700_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDF2581264.1"
                     /translation="MGKTKDVIVFGFALFAMFFGAGNLIFPPYLGIITGPEWLIAFLG
                     FTFADAGLALLAVMATAKFDGNVVEMFKRCGMKLGILIGCADILCIGPFLAIPRTGAT
                     TYEMGIMPLFGTSIPVLLFCILFFALSYVLTIRPSKVVDIVGQFLTPALLIALAFIII
                     KGIISPLGDIVDKPMIPNVFAEGIGQGYQTMDAFAAIALASVLIVSLNDKGYSTISDK
                     LKMIGKAGVLACGGLALVYGGLCFLGATVSTMYGTDAVQSQVIVNITEGLLGNVGKAI
                     LAVVVSLACLTTSIGLTSATGQYFSRLTKGKLSYEKIVLAVSVFSAVVASFGVGTIIK
                     IASPILSIVYPPSIVLIILAFFNEKIKNDNVYKGAVYMSLLVSILTVISSYGVDVPVV
                     KSLPLHSLGFNWVVPVIIAGIIGNFIPSKSQSNTLGTN"
BASE COUNT         6554 a         1477 c         2951 g         4502 t
ORIGIN      
        1 aatatagagg ataataataa aaatatagaa taagtaattt gccaaatact tgtttatatt
       61 gtataactta tgatattatg atattagtaa aaatttgtaa tcctgacttg acagaattca
      121 agtaagagat ataattttaa aggcatagta atgtaaaatt tactaaaaaa taagaaattt
      181 attttaatat ggaggaacaa aaaatggtat cagcaggtga ttttagaaaa ggtgttacat
      241 ttgaaaaaga tggacaacca tgtttagtag ttgattttca acacgttaag ccaggtaaag
      301 gagctgcttt cgttagaact aaatacaaaa acttaaaaac aggagctata agagaagaaa
      361 gtttcaatcc aagtgaaaaa ttccctaagg ctgttataga tacaagacaa atgcaatatc
      421 tatacaatga tggtgagtta tattatttta tggatcaaga aaattttgaa caaataccat
      481 taaactatga gcaagttgaa gatgcaatta agttcttaaa agaaaatgaa gttgctacaa
      541 taagatttta ccaaggacaa ccattccaag tggaagcgcc aaactttgca gagctagaag
      601 ttacagatac agagccaggt ataaaaggtg atacagcaag taatgtaact aaagcagcta
      661 cagttgaaac tggagcagtt gttcaagttc cattatttat aaatactgga gataaagtaa
      721 agatagatac tagaacaggt gaatatttat caagagtata aaaatttatg tatataaaag
      781 cttatatgtc ttaataatat aagatatatg agcttttagt ttatatataa aaaagaaagc
      841 gaggggtgtt acatgaaaca tttatatgaa gaagttgcat acttaaaagg cctagcagaa
      901 ggactagaaa taagtgctga aagtaaagaa gggaaaatga tacataaaat agttgatgca
      961 ttagaagtat ttgcagatgc tattgtaaca ttggatgaag aacaagaaga acttcaagat
     1021 tttgttgaat ctatagatga agatttagca gatttagaag aagatattgc tgacatggaa
     1081 gaagatcttt atgaagaaga tgacgatgag gatgatgagg acttcagcta tatagaaatg
     1141 gaatgcccaa attgtggaga gttagtagaa atagacgaag atttgttata tgatgatgaa
     1201 gtagatgttg tttgtcctga ttgtaaggct gtgatattat catcagaaga tgattgtgat
     1261 gatgattgta catgtggtgg ttgtccaagt tgtgatgata gagagtaaaa attcataaga
     1321 attaaaactg cctatgatgc ctatgagata ttttgcttca taggtagttt tatttttatg
     1381 aggaagggta ttatgtattt tggttttgca atacttggac aaaaatagta cactagaatt
     1441 tatagaatag gattttggag ggatgtaaat atgaaaataa gtaaaaaatt actagataat
     1501 atacaaagat ttgaaaatgt tataaattat aaatttaaaa ataaagaata tatactagaa
     1561 gctcttactc atagttcata ttctaatgaa aataaaaaat ataattttaa cgaaaggttg
     1621 gaatttttag gggattcggt tttaggcata gtgataagtg attatttatt taatgaagaa
     1681 gctaatctgc ccgaagggga actaactaaa cttagagcaa atatagtttg tgaagattcg
     1741 ttaagtgaag tagctaatga tataaattta ggtattcata tgttattagg tagaggagaa
     1801 gaagcaacag gaggaagaca taggacttca atattagcag acgcatttga agctgtaata
     1861 gctgctatat atcttgatgg aggttttgaa agtgcaagac agtttatact tcatcatatg
     1921 gaaaatataa tttatgattc aagaaaagga aatatattta gagactacaa aacgcatctt
     1981 caagaagtgt tacaagggaa tggagaaaat aatatctggt acaggcttat agaagaaaaa
     2041 ggaccagacc ataataaaag atttgtaatg gaagttggaa ttaatgacga tgtattgggt
     2101 ataggagaag gaaagagtaa aaaagaagct gaacagttag ctgctaaaat agctttaaag
     2161 aaaaaattat gggaaaaata aatataatat gtaaaaatat aaaaagaaat atttatatta
     2221 gaaggatgtt gtagatgaaa aagagaatta tacctatatt tgttcctcac aaaggatgtc
     2281 ctcatgattg catattttgt aatcaaaaaa aaataacagg tgtgagtact gatgttacta
     2341 gtgaagatgc aagaaatata attgaggaat gtttagagac tatagataaa gatgcagatg
     2401 tagaaatagc attttttggt ggtagcttta ctgctataga tattgatata caaaaaagtt
     2461 tattatcagt tgctaaagaa tatgtagaaa aaggtcttgt aaaagatatt agaatgtcta
     2521 caagaccaga ttgtattgat gaagatatat taagtatgct aaaagaatat aagacaagta
     2581 taattgaact aggagtacaa tccttagatg aaaaagtatt attagatagt ataagaggtc
     2641 atcaatctga aattgtatac aagagttcaa agatgataaa aaatagtgga atcaaacttg
     2701 gattacagat gatgattggt cttccagctg acacagaaga aaagtgtata tttacagcaa
     2761 aaaaatttgt agagctaaaa ccagattgtg ttagagtcta tccaactttg gtagtaaaag
     2821 atacaggtct tgaaaagtta atggaacaaa ataaatataa tccatttact cttgaagaga
     2881 gtgtaaatat agtaaaaaaa gttcttgtac tgttttatgt taataatatc aatgtaataa
     2941 gagttgggct tcaagctact gatgatatac aaattggaaa agctgtttta gcaggaccat
     3001 atcatcctgc atttagagag ttggtagaag cagatatgat aaaagattat ttagaatttg
     3061 ttattttgca aaataaaaat ataaagcaaa tgttggtcaa atctaataaa aagaatattt
     3121 ctaaaatcat aggaaataaa aaaacaaatg ttaaatatat ggaagaaaaa tttggtgtgc
     3181 ttttaaaaac acaagaatcc gatttagata taaatcaatt agaaattgtg ttagatggga
     3241 aaagtttaat tagtgctaat atgagagaca ttcatagaaa attgtatgac atttatgacc
     3301 tttagtttta ataatgaagg gagagaaaag atttgtattt aaaaagacta gagttaaagg
     3361 gatttaaatc ctttccagta aaaacagata ttatttttaa agaaggaata acagccatag
     3421 taggaccaaa tgggagtgga aaaagtaata tatctgatgc tgtaagatgg gtattaggag
     3481 aacagagtat taagagtctt agaggtgata aactcgaaga tgtaatattt gcaggtacag
     3541 atacaaaaaa acctatgaat tattgtgaag tggctcttac tattgataat tctgaaaatc
     3601 aattggaatt agattttaca gaagtaacta taagaagaag ggcttataga aatggtgaaa
     3661 gtgaattttt tctaaataat aaaagttgta gattaaaaga tataaaagaa gtctttttag
     3721 atactggtat tggaaaagat ggttattcaa tcatagaaca aggaaaagta gatgaaatat
     3781 taagtaataa tcctttaagc agaagaaaag tttttgatga agcttgtggc atatcaaaat
     3841 atagatataa aaaacaggaa gcagaaagga atttaagtaa tacaaaggaa aatttagaaa
     3901 gaatagatga tgtatatata gaaattgaaa atcaattaaa gcctcttttt aatcaacaga
     3961 caaaggcaaa aaagtacttg gaaataagtg aaaaattaaa gatacttgaa gtaaatagct
     4021 ttataagaga aattgaagga atagaaaaag aattaagtga ggttaatgaa catcgtaagg
     4081 ttattgaaaa ggagctcaat gaaaaagaag aacaaaaaaa tgttgtagag aaaaagcaag
     4141 aagatattaa taaagaggta gaggtattac aagatgtaat tgaaaaatct gtagattata
     4201 taaattcaat taaaggagtt atatcaaaaa aagagtccca aataaattta attaaggaaa
     4261 gaataagaaa ttttacgaat gaaattagta gaaaaaattt agaaataaat gatataaaag
     4321 agaagctaaa tgaaaataag caatacataa aagaattaga aagtaataaa ttatcaggta
     4381 gtgaagaact atctaccttg caggaaaata taaaagtatt ggaggcaagt aaagataaac
     4441 aaaaaattaa gttagaatcc ctaaataatg agatagaatt attgaaagaa agtataattg
     4501 atatattaaa taagaaacaa gagtttagta ataagctgtc cactttaaac gcaaataaag
     4561 aaaatatgaa tatcagagat gaaaatataa attcagagat aacagagtta aacaaaaata
     4621 ttgaaataaa atcatctgaa ttagatacta taaataaaga atttaatatg caaaatgaaa
     4681 atttaaagaa tgtaaataat agacataaag aattatcaat taatttgcag gattcaatta
     4741 gtgaacacaa taaactagaa gatgaaatac agaaaagtaa atataattta aatggatata
     4801 attcaaagtt aaatgtctat atagatatgg aaaatcatta tgaaggattt aatagaggag
     4861 taaaagaagt actaaaaaat aaaaatttaa aaggagttca tggagcatta ggacaaatta
     4921 taaatgtacc tgaaaaatat gagaaatcta tagaagcagc tcttggtgct tatatgcaaa
     4981 atataataac tgataatgag tttagtgcta agtctgcaat aaactatcta aaacagaata
     5041 atttaggtag agtaacattt cttccattaa atataattaa atcaaataaa gtaagtctag
     5101 gaaatttaaa agcaaatact aagttcatag gaatagcaag tgatttaatt acatttgatg
     5161 aaaagtatag aaatataatt gaaaatatac ttggaagaac aatacttata aataatatag
     5221 atgagggcat aaaatttgca aaagaaactg gtcataggtt taaaatagta actttagatg
     5281 gtgaaatctt aaatccagga ggttctttga ctgggggaag tctaaaaact aatgggaata
     5341 tactatcgag aaaaagatac attaatgaat atacagaaaa gataagtaat atcaagaatg
     5401 aaatttcaca tctagaatta aaaagagaat ctttagacaa agatgtgaaa aatatcaaaa
     5461 atgaaattga ttcacatgaa agtaaaataa aagatttaga aaaaagtata ataataaaaa
     5521 gtacaagtat aaagaatgtt gaatctgaaa ttgaatcgtt aaaaggtagt attactaaat
     5581 tagaaaatga gaagaatgat ttaaattcta atctaaatta tacacttgaa aaaagcgatg
     5641 atgttagaaa agatatggaa gagttagatg atttatacaa taaaaataaa gaaaaaattg
     5701 atgcattgaa tgaggaaata aaaagataca atgatttata tgataaagaa aaatctgaat
     5761 ttgatgaatt gaacttaagt ttagtgaaga aaacagaagc ttataatagt atagttagag
     5821 atataaaaag aatatctggt gaaaattgtg agctagaaga aaagaataaa caattagaag
     5881 agtcattaaa ttatgaagaa catgaaataa ttaaattaca agattctata gccactgaag
     5941 aaaaagaaaa agaaaattta acaaaacaac taggggatag taatagaaat cttgaaacta
     6001 gaaaaatagc aaaagatgat ttaaaaaata gttttgatga aattaataaa gaattaaaaa
     6061 ctatagatag acagcatata gagcttaaag aaagtttatt taaagttggt ggaagattag
     6121 aaagattaaa aactagtcaa gacacatata taaataagtt gtttgagcaa tatgatatga
     6181 ctttagtaca agcattggaa attaaagatg aagatttgga tatagataga aaattcttag
     6241 aaagtctaaa gagagaaata agaagtcttg gaaatataaa tataggctct ataaaggaat
     6301 atgaggaaat aaaagaaaga tacgattttt atagcgaaca aaagcaagat ttagaagagt
     6361 ctatggaaga gatagaaaaa cttatacaca ctttagaaga aaatatgaaa tctgaatttg
     6421 aaattaagtt tgaagaaata agtaaaaatt ttaaatatgt atataagaga ttatttggtg
     6481 gaggttgtgg agaattaact attttagata aagaaaattt attagaaagt gatatactaa
     6541 taacagctca acctccaggg aaaaaaatga aaaatttaaa tctattatct ggtggagaga
     6601 aagctttaac agcaataagt atattatttg ctatattgat tacaaagcca acaccatttt
     6661 gtatattaga tgagattgaa gcgccacttg atgatgcaaa tatatttaga tttggagaat
     6721 ttttgaagga tttatctaaa gagactcaat ttatatcagt aactcatagg agaggaacta
     6781 tggaagctgc tgattatatc tatggagtta caatgcaaga aaaagccatt tcaaaagtta
     6841 ttagcttaaa attaaaagaa gctcaggaaa taacagatat tatataactt agattgacat
     6901 aggaggaata acagtgttaa aaaaattatt tggttttgga aaagacaagg aaaaagaaat
     6961 agaaaaaaaa gatgctgaag aagaaataga agttgaagac agtgttgata gtttagaaaa
     7021 cttggaagaa accatttttt caggacttga agaagaagtg atagataaag tggaagatat
     7081 tgaagaaaaa actgaggaaa gtgtcaataa tgaaggcact gaagaaatcg aaaattttga
     7141 aaaaatagaa gttgagatgg atagtaatgc aaaagaagta gaaaatattt caaataatga
     7201 ttctcaagaa gagataatcg aagaattaga aaattatgat gaaattgagt ctaaagagat
     7261 agaagataaa gaagataaga aagttaactt gtttgaaaga ctaaaacaag gattgacaaa
     7321 agcaaagcaa ggaataactg atagaataga tgaagtttta aaatcatata caaaaataga
     7381 tgaagaatta cttgaagatt tagaggaaat tttaataaca gcagatgttg gtgtaaatac
     7441 aactatggat ataattgaaa gactaagaga taaaataaag caaaaaggta ttactgaacc
     7501 aataaaggta agagaagaac ttaaatctat agttgaagat attcttacaa atgaaaactc
     7561 tacactagat atagaaccag caccatgtat aatattaatg gtgggtgtaa atggggtcgg
     7621 taagactact acaataggta aactagcaaa tagatacaaa aaagatggta aaaaagtttt
     7681 attagcagca gcagatacat ttagagcagc agcaacagag caattagaaa tttgggcaaa
     7741 tagaaccaat gtagacataa taaaacatca agagggagca gaccctggag ctgtagtatt
     7801 tgatgctata aaagctgcaa aagctagaaa aacagatgta ttaatatgtg atacagcagg
     7861 aagattacac aataaggcta atcttatgaa tgaacttgga aaagtattta aaatagtaga
     7921 tagagaattt ccagaagcaa aacgtgaagt acttcttgtg gtggatgcta caacaggtca
     7981 aaatgcagtt gttcaagcaa agacatttaa agaagttgca gatataacag gtatagtact
     8041 tacaaaactt gatggaactg caaaaggtgg agtagtcctt gcagtaaaat cagaagtaga
     8101 tgtacctgta aaacttattg gagttggaga aagtgtagaa gacctacaag atttcaatgc
     8161 aaaatcattt tcagatgcat tatttggtaa ctaatgttct tttaaaaata atggttgaca
     8221 agagtgttgt tatgatataa aatacaatag tgtaaagtaa ataaccttta cagtatactg
     8281 ctaggaagtg taagatatga atatagaaaa aatggtcgaa attggattgt tatttgaaca
     8341 gtataaagaa ttattaactg acaagcagaa agaaatagtt gctctatact atgaagaaga
     8401 ttattcttta ggtgaaatta gtgaaaatct gaatgtatca agacaaggtg tttatgatac
     8461 cttaaagcgt tcagaaaaaa tactaagaga ttatgaagaa aagttacact tagtatcaaa
     8521 gattcaagag caggaaaaaa atattaagat tataaaagat aaaattattg acattaaaga
     8581 agatttattg cacaatagag attgtgctaa tttaatccct aagctagaaa atatagaaga
     8641 tgtatgtagg gagatgataa aatgatattt gaaggattgt ctgataaact acaaggggca
     8701 tttagtaagt taaaatctaa aggaaaactt acagaagtag atgtaaaaaa tgctatgaga
     8761 gaagttaaac ttgctcttct tgaggcagat gttaacttta aagtagttaa agatttcata
     8821 aaaaaagttc aagagagatg tgttggacaa gaagttatgc aaagcttaac tccagctcag
     8881 catgtaatca aaattgtaaa tgaagagctt acaagtttaa tgggagatgt acaaagtaaa
     8941 gtaatgattt cctcaaagcc tcctacaatt ttaatgatgg ttggattgca aggtgcaggt
     9001 aagactacta cagcaggaaa acttggagga tattttaaaa agcaaggtaa aaaacctcta
     9061 ttagtagctt gtgatattta tagaccggca gctataaagc aacttcaagt tgttggagaa
     9121 aaattggata tacctgtatt caatatggga gataaagaaa atccagtaaa tattgcgaaa
     9181 gcaggtttaa gccatgcgat taaaaatgct aatgatttag ttattataga tacagctggt
     9241 agacttcata tagatgaagt acttatggct gagttaaagt ctattaaatc agaagttaag
     9301 ccacatgaga tacttttggt tgtagattct atgacaggtc aagatgctgt aaatgttgca
     9361 gaaagcttca atgaggcact tggtgttgat ggagtagtat taactaaatt ggatggagat
     9421 actagaggtg gtgctgcact ttcaataaga gcagttacac aaaagcctat aaaatttatg
     9481 gggatgggag aaaagctaga tgatatagaa cctttccacc cagatagaat ggcatcaaga
     9541 atattaggta tgggagatgt cttaagttta atagaaaaag cacaagaaaa tatagactta
     9601 gagaaagcta aagaattaga gcaaaaaatt aaaaaacaag agcttgattt tgaagatttc
     9661 ttacaacaaa tggaacaaat acaaaatatg ggacctcttg acaagatact tggtatgata
     9721 cctggtatgg gaaatgtaaa agaccaactt ggagatgtag atttgaatgg caaagaaatg
     9781 aagagaacaa aagccataat tcagtcaatg actacatatg agagaagaaa cccaggagta
     9841 ttaaatgctt caagaaagaa aagaatagct aagggcagtg gaactagtgt acaagatgta
     9901 aatagactta taaaacaact taatgaaatg aaaaaaatga tgaagatgtt tgctggttca
     9961 caaaagagta tgaagaaaag gggcggctta ggcggtttac ctttttttaa ataaaatata
    10021 aaaaagtaaa aattattcgg aggtgactgt aatggcagtt aaaataagat taaaaagaat
    10081 gggagctaac aaaaaacctt tctacagaat agtagtagct gattcaagag ctccaagaga
    10141 tggaaaattt atagaagaaa taggatacta taatccaatt tctgaaccta aacaagtaag
    10201 aataaatgat gaaaaagcta taaaatggtt agctactggt gctcagccaa cagaagtagt
    10261 taaaaaatta cttgtaaaaa acggagtaat agaaaaattc gaagcttcta aacaagcaaa
    10321 ataatttttt cttggcaatt agtttaagta agggctcatt gctaaggtta ctttaggaga
    10381 tgaaatatat gaaagagcta gttgtagata tagctaaggc tctagttgat aatcctgact
    10441 cagttgttgt tgaggaattt gaagataatg atggtatcgt cttaaagctt acagttgctc
    10501 aagaggatat gggtaaggtt attgggaagc agggaagaat agctaaagct ataagaactg
    10561 tcgtaagatc tgttgcaaat agagaaaata taaaagtttc tcttgagata gtataaaata
    10621 ggaggattag gtataatgcc tagtcctttt tatataaatg cgtagtttaa gattttatga
    10681 tgatatgacc acataagatt aaatttttta agaaggtgat aacatgaaag aaaaattaac
    10741 tcattttaag gtaggtcaaa tagtaaatac acaaggttta aaaggtgaag taagagttta
    10801 tccacttact gatgatatag agaggtttga tgaattaaaa gatttctatc tgggaaaaga
    10861 tttaaataat aaattggctg ttgaaaaagt aagatacaaa ggttctatgg ttataatgaa
    10921 gataaaaggc atagacagca tagagaaagc agaaaaacta aaaaataaat ttatgtatgt
    10981 atctagagaa gatagcagag agcttgaaga aggcgaattt tttatagcag atatgattgg
    11041 aatggaagtt ttaactgtag atggaaaata tgtaggaata ctagaagatg ttcttcaata
    11101 ctcagcaaat gatgtttatg tagtaaaagg tgaagaagat aaagaattta tgataccagc
    11161 tataaaaaaa tttgtaccta caatagatat tgatgaaaga aaaatgatta tagaccctat
    11221 taagggaatg atagattagg tggtaatatg agatttcata taatgacact ttttccagaa
    11281 atatttaatt cgtacatgga tgaaagtata atgaagagag cagttgaaaa aggtataatt
    11341 gaggtttata tatataatat aagggatttt tctaataata aacataaaaa agtagatgat
    11401 tatccatttg gaggaggagt ggggatggta atgactcctc aacctatata tgacacatat
    11461 aagcatataa taacaactca caatataagt aatccaagtg taatttatct tactccaaaa
    11521 ggtaaggtat acaatcaatc tatagcaaag cagatgtctt taaaagaaga tataatacta
    11581 ttatgtggcc attatgaagg agtagatgag agaatcattg atttaatagt tactgatgaa
    11641 atttcaattg gagattatgt attaactggg ggagagctcc cagcacttat aatgatagat
    11701 tctatatcaa gattaatacc aggtgtatta aaccaagaag aatcatttga agaagaatca
    11761 tttaaagata acttattgga atatcctcat tacactagac ctagagattt tgaaggatta
    11821 aaagtaccag aagtgctttt atcagggaat cataaaaaga tagatgagtg gagaagagaa
    11881 gaatctataa ggataaccaa agaaagacga tttgatttat ataaaaagtc taatgaaaaa
    11941 taaaaatata atgaaaaatt aatatatatg tatacaataa agtacctata aatggaatta
    12001 aaaacataaa agtgcttgta ataactattg aagtatagta taatataaaa tgttgaaaat
    12061 acgggcgttc ctctgtttac actggtcggg tagttaagaa cgtctatgat atctaggagg
    12121 aagaaagatg aatgaaatgc taagagcaat tgagcaagaa caattaaaaa atgaagttcc
    12181 taattttgga cctggggaca cagttaaagt acacgttaga attatagaag gaaaaagaga
    12241 aagaatacaa gtattcgaag gtgtagtatt aaagagacaa ggtggaggag caagagaaac
    12301 tttcacagtt agaaaaatgt ctttcaatgt tggtgtagaa agaactttcc cagttcactc
    12361 tccaaaaata gaaaaaatag aagttactag aaaaggtaaa gtaagaagag ctaaactaaa
    12421 ctacttaaga ggtagagtag gtaaagctgc taagataaaa gaagctagaa ataaataatt
    12481 aaaaacttta cttataaatg gggggctgat ttatcagtcc tctttctatt ttaatgaaaa
    12541 ttacagcaaa taattatact agaaataaat agaaatattt gttgaaaata tacattattt
    12601 atctatgaat ttatagcttt taaaatgaaa aataaaattg gtaaatattg gattatattt
    12661 tgtaacgata ttaaaaataa gagaacagta ttatgtaaag tatgacgtta aaaagatgac
    12721 aatattatag tttgtgaaga tagatttttt aagtggaatt agaatataat aatataatat
    12781 taataaggag tgatacatgt gtcaaatgaa tacgaagagt atcttatgga tgacaatcta
    12841 catataaact ggtatccagg tcatatgaaa aaaactaagg agttagtaaa aaataactta
    12901 aaattgattg atgtagtaat tgaacttttg gatgctagaa tcccgtttag tagtaaaaat
    12961 ccagacatag acaggcttgt aggagataag ccaagagttg tcgtacttaa taagagtgat
    13021 atggcagata gggataaatt gaatcaatgg attgaatact ataagaaaat taatatcaaa
    13081 gctataccag tagatacaat aaaaggtgtt ggtataaata atataatcga agaatgtaaa
    13141 aatgtaacta gagaaaaaat gagttcttta aaagacaaag ggagaaaaga aagagctata
    13201 cgaataatga tagttggagt tccaaacgtt ggtaaatcgt ctcttataaa caaattaaca
    13261 ggtagaaaaa gcactcaaac tggagataag ccaggagtaa cgaaagggaa acaatgggtt
    13321 agattaaagg gaaatcttga acttttagat acacctggaa tactttggcc taagtttgaa
    13381 gaccaagaag ttgcattaaa tttagcattt agtagagcta taaaggatga gattttagat
    13441 actgagactt tggctttaag acttatagaa aaacttatga aaatagaacc tgagaagttg
    13501 aaagccagat ataagttaga ttgtctagga gaaactccaa ttgaaactat ggatatgatt
    13561 ggccataaaa gagggtttat aacaggtaaa aaagaactag attatacacg catagccgca
    13621 actgtattaa atgaatttag agatggaaaa ataggaaata taacattgga agttccagaa
    13681 aacgttaaaa gatagatttt aaaacaaaat caaagattta atgttaaaaa aataaaaaag
    13741 ccttttagca aaggcttttt tatttttttg aaatatagac aaatatctcc atataaaagt
    13801 ataaattagg ttagaaaacc tttaaaaata ttaatttaag acaaatacac ctaatatttt
    13861 gaaaattaaa atgagtgtga ttattttaaa ttttagcata ttgacttgct tgtttcgtat
    13921 aatatataat aagcacaaat aaatacaata tattaaaaat ttaatttatt taattttatt
    13981 aatttaataa taagttctta ttgtttaatt taatttattt atgcaagcgt aaacaatcga
    14041 gacattttat taatttaata aggttaaaaa gggggatttt ataatgggta agaccaagga
    14101 tgttatagta tttggatttg cgttatttgc aatgttcttt ggagcaggta acttaatatt
    14161 tccaccttac ttgggaataa ttacaggtcc agaatggtta attgccttct taggatttac
    14221 gtttgctgat gctggattgg cacttctagc agtaatggct acagctaaat tcgatggaaa
    14281 cgtagttgaa atgtttaaaa gatgtggtat gaagttaggg attcttatag gttgtgcaga
    14341 cattttatgt ataggtccat tcttagctat accaagaaca ggtgcaacaa cttatgaaat
    14401 gggtataatg cctttatttg gaacttcaat tccagtgtta ttattctgta tacttttttt
    14461 tgcgctttca tatgtactaa caataagacc ttctaaggtc gtagatatag ttggacagtt
    14521 tttaactcca gctcttttaa tagctctagc atttataatt ataaaaggta ttatatcacc
    14581 tcttggagat atagtagata aacctatgat acctaatgta tttgcagaag gtataggtca
    14641 aggatatcag acaatggatg cttttgcagc aatagcactt gcatcagtgt taatagtatc
    14701 tttaaatgat aaaggatatt ctactattag tgacaagtta aaaatgattg gtaaggcggg
    14761 ggttcttgct tgtggaggat tagctctagt ttatggagga ttatgtttcc ttggagcaac
    14821 tgtatcaaca atgtatggaa cagatgcagt acaatcacaa gtaatagtaa atataactga
    14881 aggattactt ggaaatgttg ggaaagcgat tttagcagta gtagtatcat tagcatgttt
    14941 aactacatct ataggtctta catctgccac aggtcaatat ttctctagac ttacaaaagg
    15001 taaattgagt tatgaaaaaa tagtattagc tgtatctgta ttcagtgcag tagtagcatc
    15061 ttttggtgtt ggaactataa taaagatagc atctcctata ctaagtatag tttatccacc
    15121 atcaattgtt ttaataattt tagctttctt caatgaaaaa attaagaatg ataatgtgta
    15181 caaaggagca gtatatatgt cattgcttgt aagtatacta acagttatat ctagttatgg
    15241 agtagatgtt ccagtagtaa aatctttacc tcttcattca ttaggattta actgggtggt
    15301 accagttata atagctggaa taattggtaa ttttatacca tcaaagagtc aatctaatac
    15361 acttggaaca aactaagttt tgtttaatta aaatataatc aattgtaaat aaatgagtta
    15421 actaaaagta aaaggagtta tttgtacaaa taactccttt tattttggga ttctataatt
    15481 aatt
//
LOCUS       DAMJWP010000077        15446 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.081, whole genome shotgun sequence.
ACCESSION   DAMJWP010000077 DAMJWP010000000
VERSION     DAMJWP010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15446
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.081"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(47..781)
                     /locus_tag="PC700_001954"
     CDS             complement(47..781)
                     /locus_tag="PC700_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis protein TtcA"
                     /protein_id="HDF2581265.1"
                     /translation="MSIQKLLSKARKAIQDFDMIQENDKIAVGLSGGKDSLTLLHILK
                     SYQRFSPQNFELIAITLNTGGVDNSPLDKLCKEINVPFYEFQTDIKEIVFDIRQEKNP
                     CALCANLRRGALNDNAKKLGCNKVALGHHKDDAIETFLMSMFYEGRVNCFSPKTYLDR
                     QDLTVIRPMIYIEEYMTKKISKDSNYPIITNPCPANGHTRREYIKNLIANLNKEMPDF
                     KRNVFGALNNSEKLFIWDKEKIKNFK"
     gene            907..1623
                     /locus_tag="PC700_001955"
     CDS             907..1623
                     /locus_tag="PC700_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF2581266.1"
                     /translation="MKKFKLIGAIILVVFISVFISKDRLLKKDKDPIVAAQHDSEQDV
                     EANSQNKNHSNSISNILLVNKTNGISKNYTPENITKVNIPFVEEATEEEKQMAGEPAK
                     AVEDLVKQANSEGIQFLGSSAYRSYDTQLDTYTRRVKSQGREKADAYVAKPGYSEHQT
                     GLCIDLTNPERWFVGSTKEAKWLAENAHKFGFIIRYPEGKEDITGTAYEPWHIRYVGK
                     DAAEEIYSKGLTLEEYLQNK"
     gene            1757..2449
                     /locus_tag="PC700_001956"
     CDS             1757..2449
                     /locus_tag="PC700_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2581267.1"
                     /translation="MNNTVLVVDDEKEIRDLIEIYLLNSGYNVIKAEDGKEALDILKK
                     ENIHLMILDIMMPKMDGIEVCRKVRESLNIPILMLSAKSEDMDKIQGIMTGADDYLTK
                     PFNPLELTVRVKSLIRRAYYFSGANNEDKDVIKVGVVIIDKEKHSVTVAGNEIILTSR
                     EFDILYLLANNKGRVYSTEEIFEKVWKEKYYQSNNTVMVHMSRIRDKIEKYTDGEKII
                     HTVWGVGYKIEK"
     gene            2439..3620
                     /locus_tag="PC700_001957"
     CDS             2439..3620
                     /locus_tag="PC700_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HDF2581268.1"
                     /translation="MKNKSLLNHMFRPILDLIISCALTSMLILTSMYIIKLLYFNSEI
                     SVINQLVYQLRDFRSLIGESLFYPFLFTITSLAIYSMISYKRNKQIVDFIRKTNRKNQ
                     KLEIIKSAVNLMDEGNLEKSIDIEYDLDILKDKEKDDIDELAHKINNIVHQLRNITIE
                     ERQAQQTKTDLITNVSHDLRTPLTSIMGYLGVIEEGKYKDEVQMMYYVDIAYEKSKNL
                     NVLINDLFELTKMQNNTIKLNKIEINLVELLSQVVSQFEIYFKQEFMVSRINFNEEKL
                     IVKADPNKLVRAFENLITNAVKYGKDGHYVDIVTEKMEDMAVVKVINYGEPIPVLDLP
                     NIFDRFYRVEKSRNRNVGGSGLGLAITKNIVNLHDGEITVSSDKYQTVFEVQLPIIQS
                     A"
     gene            3902..4357
                     /locus_tag="PC700_001958"
     CDS             3902..4357
                     /locus_tag="PC700_001958"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDF2581269.1"
                     /translation="MEKVESFKLDHTKVKAPFVRKCSVLDGVKGDKVTKFDLRFLQPN
                     VESFGTAAMHGLEHLLATYLRDTLDGVIDLSPMGCRTGFYLILWGDVDAKTVKIGLEE
                     ALKKVLESDKMPAATAIECGNYRDLSLFGAKEYAKDVLDKGFSLNIYGE"
     gene            4347..5072
                     /locus_tag="PC700_001959"
     CDS             4347..5072
                     /locus_tag="PC700_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vitamin B12 dependent methionine synthase,
                     activation domain protein"
                     /protein_id="HDF2581270.1"
                     /translation="MENNLIGREDISINQSEVLRYLQYRGQEIDKDLKYTIDECVAIT
                     KNKINPRYLSRIYPIRINKDNVVELEGTNLTLESKDVYELLKECNECVIMAATIGIDI
                     EKEIRRHSYSNLTKGLIIDSCGTTAIEEVCDIVQNNLEKVFLNQDKFITMRYSPGYGD
                     LPIEKNIDILNVLNAQKQIGLTITSNGIMIPRKSVVAIIGISNTRINKSKKEKSCENC
                     KNYRNCTYRKGANSCGNKTISEK"
     gene            5044..7425
                     /locus_tag="PC700_001960"
     CDS             5044..7425
                     /locus_tag="PC700_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HDF2581271.1"
                     /translation="MEIRQYLKNNILIFDGAMGTMLQQKGLKLGENPEVFGLQNPDKL
                     IEIHTAYLEAGSNVILTNTFGCNELKLDSKYTVEEVIDNAVLVARKAIENVDNTKPRY
                     VALDIGPIGEMLEPMGTLSFDNAYEIFKRQVLQGVKSGVDVIVIETMMDLYEAKVAVL
                     AAKENSDLPIFCTMTFDEGGRSFTGCMPECMVATIEGLGVDAIGVNCSLGPKQLLPIV
                     EKIASRATVPVMVQANAGLPNIVDGEAIYDVDAKEFFEGVKKFVEVGATIIGGCCGTN
                     PSFIKEISENINSVTKGCIEKIDKCVVCSPSKFVEVQSPTVVGERLNPTGRKSLQEAL
                     KNENVDYAINLGLEQVNAGAQILGVNVGLPEIDEKKLMPKLIREIQAVVDTPLQVDSS
                     NVEALEQGLRYYNGRTIVNSVNGKEESLESILPIVKKYGSCVVGLTLDEKGIPKKAEE
                     RFEIAKRIVNRAVSCGIKPKDIFIDCLSLTVSAQQEEAIETIKAITMVKTLGVKTILG
                     VSNISFGLPNRKALNASFLTLALGAGLDLAIINPNEYSMMEAINSFKILNNTDKGCIN
                     YINQYSNINNSKKSDSSTKIDKDLPLDILVERGLKDEAKNVTLNLLKEKDENYILDEI
                     LIPALDKVGKRYDSGDIFLPQLIQSAETVKVSLNTIKETLLSKSSNNVSKGKIIVATV
                     KGDIHDIGKNIVKIMLENYGYEVIDLGKDVPIEEVVEVAKKRNIKLVGLSALMTTTVQ
                     SMKDTIQALRDNEINAKVFVGGAVLTEEYAEEMGADYYSKDAKSAVEIAKLNF"
     gene            complement(7630..8859)
                     /locus_tag="PC700_001961"
     CDS             complement(7630..8859)
                     /locus_tag="PC700_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDF2581272.1"
                     /translation="MNLNRNLEKYAELAIKVGVNIQPGQILLIKSPIECADFARNALK
                     EAYKCGAKNVYIEWSDEESTLIKYLYAPDEAFHEFPKWTAEQYVDIAKEGGAFLSVYA
                     QNPDLLKDVDPERVANFQKASGKALKEWRSYTLTDKCKWSIVSVPTKDWAKKVFPNLS
                     EDKAIEKLWDAIFKCSRVDGQDPIKAWEEHNENLKSKMDFLNKNNFKTLKYKSSKTDL
                     TLDLPKGHVWLSGASKDPNGISFNPNIPTEEIFGMPHKFKVNGTVYSTKPLVYGGNII
                     DNFFLTFKDGKIIDFSAEKGLDTLEKLIETDEGSHYLGEVALVPYNSPISNTDIIFYN
                     TLYDENASCHFAIGSAYKTCLEGGNNLKDEELDEHGVNDSLTHVDFMIGSADMDIIGE
                     TYDGKQIQIFKNGNWAF"
     gene            9459..10373
                     /locus_tag="PC700_001962"
     CDS             9459..10373
                     /locus_tag="PC700_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581273.1"
                     /translation="MELYEAIFYRKSVRNFSSKKIKKPLMEEIKRSCKNLEYLNEDLN
                     IKAHVVDRGHIIHFLMGKECKVKAPHYIVVTSDKGEDYLQNIGFATEGVVLRLTTLGI
                     ATCWLEGNLKREDILEFVDIGNKNFEEDEEDDLDYLAGVKTEEDDENKIENPYIIIAF
                     GYPAKNEELFRTRYARPDRKPVKDMINKINRNRKWKDILELTRRAPSVKNSQPWYFHK
                     DERGLHLFEKRPKKHCEDMNKVSLGVALRHFDIACIKNKIDVSYEKLPIRNKIGKSYF
                     ITVVEHVKPEEETQEENVTLEKEESQDE"
     gene            10397..11032
                     /locus_tag="PC700_001963"
     CDS             10397..11032
                     /locus_tag="PC700_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HDF2581274.1"
                     /translation="MYKCGKCNLRGCSNGNMDKLMKICPTREIELQQKAKQLYEEEEN
                     RKIAYNAGLVEAEGYCQYTRLKEIIEFAKKCGYKKIGIAFCIGLKREMQTAQKIFEHH
                     GFEVFSVICKNGAMPKSTIGLEASQTISKCSDEVMCNPIGQALFLNEAKVDLNVILGL
                     CVGHDTLAMKYMEAPITTLVVKDRVTGHNPVAAIYNAESYFEKKLFDKDEE"
     gene            11408..12124
                     /gene="pyrF"
                     /locus_tag="PC700_001964"
     CDS             11408..12124
                     /gene="pyrF"
                     /locus_tag="PC700_001964"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDF2581275.1"
                     /translation="MINGKEKLIVAIDTDEFDKAKELIDRLEDSVDIFKVGLEQYVAT
                     KGKTIDYLKEKGKKIFLDLKFHDIPNTMKSAVRAAVRDNVWLMTIHVSDMEGMRQCAL
                     IAREEAERLNIEKPLVVGVTVLTSLSNQDLQDIGCNMTTEELAIKRAKLAKESGIDGV
                     VCSAQEVDKIVEACGADFITVCPGIRPKSAEVGDQKRVVTPSDAIKKGAHYLVVGRPI
                     TKAENPRESAINIVREIENA"
     gene            12150..13193
                     /gene="carA"
                     /locus_tag="PC700_001965"
     CDS             12150..13193
                     /gene="carA"
                     /locus_tag="PC700_001965"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDF2581276.1"
                     /translation="MKGKLILEDGTVFEGKAFGYLKEAIGEVVFNTSMAGYGEILTDA
                     TYYGQIVTMTYPLIGNYGINLEDVESNKVQVKGLIVREKSDNPNNFRCEMDIDTYLKQ
                     NKVIGLEGIDTRALTKIIRNNGTKRGIITLENVSLQDVQSKLEEHCNKDSVKNVTRKE
                     IENIKGTGAKVVVIDFGVKNSVLESLKACNCDITIAPAMTTVEEILNINPDLILLSNG
                     PGNPEDINDIVENIKKLIGKKPLTGIGLGHQILALALGGKTSKLVFGHRGGNHPVKCL
                     ETGKVFITSQNHGYNVSEMPENTEVTHINLNDNTIEGMRHKELPIYSVQYIPEYTTKE
                     DLDCIFNKFLALI"
     gene            13246..>15446
                     /gene="carB"
                     /locus_tag="PC700_001966"
     CDS             13246..>15446
                     /gene="carB"
                     /locus_tag="PC700_001966"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HDF2581277.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQSLKEEGIEV
                     VLINSNPATIMTDKEVADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     YEKGILDKYGVKIIGTSVESIKKGEDRDIFREVMKEINQPVIVSDIVTDLQAGLDYAL
                     TIGYPVVVRPAYTLGGTGGGIADNEEELREILSHGLQLSPVGQVLIEKSIKGWKEIEY
                     EVIRDSKGNCIVVCNMENIDPVGVHTGDSIVVAPTQTLSNKECAMLKKASLDILNAVE
                     VQGGCNVQFALNPHSFEYAVIEINPRVSRSSALASKATGYPIAKVSSKIALGYTLDEI
                     ENAVTKKTYACFEPTIDYVVAKIPKWPFDKFKKANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHTPSEERSIEELKKRVVVPDDERLFDLAEMIRRGYKVEMIEQ
                     ITGIDKWFINKFKWIVEQEEKLKVMKIEDLTKDYLLELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIKPAYKMVDTCAGEIDAISPYYYSTYEQYDEVVVSDKKKVIVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMDIETIIINNNPETVSTDFDTSDKLYFEPLTEEEVLSI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFDDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWSLNEGIEVANELGYPVLVRPSYVLGGQGMEITYNEEKLSQYL"
BASE COUNT         6207 a         1713 c         2785 g         4741 t
ORIGIN      
        1 tttataatta atacaatctt gcttttttat ttaaaaattt taattattat ttaaaatttt
       61 ttatcttctc tttatcccat ataaacaatt tctcagaatt atttaaagcg ccaaatacat
      121 ttcttttaaa atcaggcatc tctttattta aattagcaat aagatttttt atgtactctc
      181 tcctagtatg accatttgca gggcagggat ttgtgataat aggatagtta gaatcttttg
      241 aaattttctt agtcatatac tcttctatgt aaatcatagg tcttataact gttaaatcct
      301 gtctatctag gtatgtcttt ggggaaaaac aatttactct accctcataa aacatagaca
      361 ttaaaaatgt ctcaattgca tcatctttat gatgaccaag agctacttta ttacatccaa
      421 gttttttagc attatcgttc aaagcacctc ttcttaaatt agcacataat gcacaaggat
      481 ttttttcttg tcttatatca aatacaattt cttttatgtc agtttgaaat tcataaaaag
      541 gaacatttat ttctttacac aatttatcta gtggagaatt atcaacacct ccagtattta
      601 atgttatggc tatcaattca aagttttgtg gagaaaatct ttgataactt ttaagtatat
      661 gaagtagagt taaactatct tttccaccag aaagtccaac cgcaatttta tcattttctt
      721 gtatcatatc aaaatcttgt atagccttac gagctttact tagaagtttt tgtatagaca
      781 ttttatcacc atcctttaaa aatattaaat ttaagattta tttaagattt acttataaaa
      841 tacttaagtc ttatactcta aaattaacat tatagacaaa cataacaaaa aggagaagat
      901 ttaattatga aaaaatttaa attaatagga gccattattt tagtagtatt tataagtgta
      961 tttatatcta aagatagatt acttaagaaa gataaagatc caatagtagc tgctcaacat
     1021 gattcagaac aagatgttga ggcaaattct caaaataaaa atcactcaaa tagtatatca
     1081 aatatattat tggtaaacaa gacaaatgga attagcaaaa actatactcc tgaaaatatt
     1141 actaaggtaa acataccttt tgttgaagaa gcaacagagg aagaaaaaca aatggcagga
     1201 gaacctgcaa aggcagttga agatttagtt aagcaagcta attcagaagg aatacagttt
     1261 ttagggtctt cagcatacag atcttatgat actcaactgg atacttatac aagaagggta
     1321 aaatctcagg gacgagaaaa ggcagatgca tatgtagcaa agcctggtta cagtgagcac
     1381 caaactggac tttgtattga tttaacaaat ccagaaagat ggtttgttgg aagtacaaaa
     1441 gaagctaaat ggttggcaga aaatgctcac aagtttggat ttataataag atatcctgaa
     1501 ggtaaagaag atataacagg tacagcatat gaaccatggc atattagata tgttggtaag
     1561 gatgctgccg aggaaattta ttcaaaagga ttaactttag aagaatattt acaaaataaa
     1621 taatataaat gtgtaaataa tttgtattat attggaaatt tagaaatgaa taaatttata
     1681 gattacacta actaaaatat agtataattt tatatgaata aagctatata caaagattaa
     1741 gttaggatgt gtaaaaatga ataatacagt tcttgttgtg gatgatgaaa aggaaataag
     1801 agaccttata gagatatatc ttttaaatag tggatataat gtaattaaag ctgaagatgg
     1861 aaaagaggct ctagatattt taaaaaagga aaatattcat ttaatgatat tggatataat
     1921 gatgcctaaa atggatggga tagaagtttg tagaaaagtt agagaaagtt tgaatatacc
     1981 aatacttatg ttaagtgcaa aatcagaaga tatggataaa atacaaggga taatgactgg
     2041 tgcagatgat tatttgacaa aaccatttaa cccacttgaa cttactgtga gagtgaaatc
     2101 actgattaga agagcatatt attttagtgg tgctaataat gaagataaag atgtcataaa
     2161 ggttggagta gtaattatag ataaggaaaa acatagtgta acagtggctg gaaatgaaat
     2221 aattttgaca tcaagagaat ttgatatatt atatttacta gcaaataata aaggcagagt
     2281 atatagtaca gaagaaatat ttgaaaaggt atggaaagag aaatattacc aatctaataa
     2341 tactgtaatg gttcatatga gcagaataag ggataaaatt gaaaaatata cagatggaga
     2401 aaaaataatt catactgtat ggggagtagg atataaaatt gaaaaataaa agtttattaa
     2461 atcatatgtt tcgacccata ttagatctta taataagctg tgctttaact tcaatgctta
     2521 ttttaacaag tatgtatata ataaaattat tatatttcaa tagtgagata tctgtaatta
     2581 atcaacttgt atatcagtta agagatttca gaagtcttat aggagagagt ttattttatc
     2641 catttttatt tacaatcaca tcattagcaa tttattccat gatatcgtac aagagaaata
     2701 agcaaattgt agattttata agaaagacta atagaaaaaa tcaaaaatta gaaatcatta
     2761 aaagtgctgt aaatttaatg gatgaaggta acttagaaaa aagtatagat attgagtatg
     2821 atttagatat attaaaggat aaagaaaagg atgatattga tgaactagca cataaaatca
     2881 ataatatagt ccatcaactt agaaatataa ctattgaaga gagacaagct cagcagacaa
     2941 aaacagattt aataacaaat gtgtcacatg acttaagaac acctttgaca tccataatgg
     3001 gttatttagg agtgatagaa gaaggtaagt ataaagatga agttcagatg atgtattatg
     3061 ttgatattgc atatgaaaaa tcgaaaaatt taaatgttct tataaatgat ttgttcgaat
     3121 tgactaaaat gcaaaataat acaataaaac taaacaaaat agaaataaat ttagtagagt
     3181 tattaagtca agtagtatct caatttgaaa tttattttaa acaagaattt atggttagta
     3241 gaattaattt taatgaagaa aaattaattg tgaaagctga tccaaataaa ttggtaagag
     3301 catttgaaaa tcttataact aatgctgtga aatatggaaa agatgggcat tatgtagata
     3361 tagtcacaga gaaaatggaa gatatggcgg ttgtaaaagt gataaattat ggagaaccaa
     3421 ttccagtgtt agatttacca aatatatttg atagatttta tagagttgaa aaatcgagaa
     3481 atagaaatgt tggaggttct ggattgggtc ttgcgattac aaaaaatatt gtaaatttac
     3541 atgatggaga aattactgtt tcaagcgata aatatcaaac tgtgtttgag gtacaactgc
     3601 caataataca atcagcttag ttataaaata aaagtacgat tatgtgatat aaatattata
     3661 acaataaagt gtttacaaaa ataaaaaagt gatatataat ttaagtcagt ttaataagat
     3721 acttatcaag aattagggga gagagttagc tcagtgatcc tataccaacc ttttatcagg
     3781 tggtcctgct aacaacgatg agatgacttg aaataatata ctcttcttat caataataga
     3841 taggaagagt tttttttaga attaatgtaa caaactttaa agtataatgg aggattaaat
     3901 aatggaaaaa gtagaaagtt ttaagctaga ccatacaaaa gtaaaagctc cttttgtaag
     3961 aaagtgtagt gtacttgatg gagtaaaggg agataaagta actaaatttg acttgagatt
     4021 tttacagcca aatgtagaaa gttttggaac tgctgctatg cacggtttag agcatctttt
     4081 agctacttat ttaagagata cattagatgg tgtaatagac ttatctccaa tgggatgtag
     4141 aacaggattt tatttaatac tatggggaga tgtagatgcc aaaacagtta agattggttt
     4201 agaagaagca ttaaaaaaag ttcttgaatc agataaaatg ccagcagcta cagccataga
     4261 atgtggaaac tacagagact tatctttatt tggggcaaag gaatatgcaa aagatgtttt
     4321 agataaagga ttttctttaa atatatatgg agaataattt aattggaaga gaagatataa
     4381 gcataaatca aagtgaagtt ttaagatatt tacaatacag aggtcaagag atagataaag
     4441 accttaaata tactattgat gaatgtgtag ctataactaa aaataaaata aatcctagat
     4501 atttatctag gatttatcct attagaataa ataaagataa tgttgtagaa ttagagggaa
     4561 caaatttaac attagagagt aaagatgtat atgaactttt aaaagaatgt aatgagtgtg
     4621 taattatggc agctactata gggatagata tagaaaaaga aataagacga cactcttatt
     4681 caaatttaac aaaaggacta ataattgatt catgtggaac aacagcaata gaagaagtgt
     4741 gtgacattgt tcaaaataat ttagaaaagg tttttttaaa ccaagataaa tttataacta
     4801 tgcgatatag cccaggatat ggagatttac caatagaaaa aaatatagat atattaaacg
     4861 ttttgaatgc tcaaaaacaa ataggtctaa caattacttc taatggaatt atgataccga
     4921 gaaagtcagt agtggctatt ataggtattt ctaatacaag aataaataaa tctaagaaag
     4981 aaaagtcgtg tgaaaattgt aaaaactata gaaactgtac atatagaaaa ggagcgaact
     5041 cttgtggaaa taagacaata tctgaaaaat aatatattaa tatttgatgg tgcaatggga
     5101 acaatgcttc aacaaaaagg gttaaagtta ggtgaaaatc cagaagtatt tggtcttcaa
     5161 aatccagata aacttataga aatacataca gcatacttag aggcaggttc gaatgtaatt
     5221 cttacaaata cttttggatg taatgaacta aaattagata gtaagtatac tgtagaagag
     5281 gtgattgaca atgctgtttt ggtggctaga aaagctatag aaaatgtaga taatactaaa
     5341 ccaagatatg tagctttaga tattggtcct ataggagaaa tgctagagcc tatgggtact
     5401 ttaagttttg ataatgcata tgagatattt aaaagacaag tactacaagg cgttaaatct
     5461 ggagttgatg taatagtaat agagactatg atggatttat atgaagctaa agtagctgta
     5521 ttagcagcaa aagaaaactc tgatttacct attttctgta ctatgacatt tgatgaaggt
     5581 ggaagaagtt ttactggatg tatgccagaa tgtatggtgg ctactataga aggtctaggt
     5641 gtagatgcaa taggagttaa ttgttcatta ggacctaaac aacttttacc aatagttgaa
     5701 aaaatagcat caagagctac agtccctgtt atggttcaag caaatgcagg tcttccaaat
     5761 atagttgatg gagaggctat atatgatgtt gatgcaaaag aattttttga aggggttaaa
     5821 aagttcgttg aagttggtgc tactataata ggtggatgtt gtggtacgaa tccaagtttt
     5881 ataaaagaaa tatctgagaa tataaattct gttacaaagg gatgtatcga gaaaattgat
     5941 aaatgtgtag tctgttcacc atcaaaattt gtggaagtac aatcgcctac agttgttgga
     6001 gaaagattga atcccacagg tagaaaatct ctacaagaag cattaaaaaa tgaaaatgtt
     6061 gattatgcta taaatttagg cttagaacaa gtaaatgctg gagcacaaat acttggagta
     6121 aatgttggtc ttccagagat tgatgaaaaa aaactaatgc caaaattaat aagagagatt
     6181 caagcagtag tagatacacc tttacaagtt gattcttcca atgtagaggc cttagagcaa
     6241 ggacttagat attataatgg tagaacaata gtaaactctg taaatggcaa agaggagtct
     6301 ttagaaagta tattaccaat agttaaaaag tatggttcat gtgtggttgg acttacactt
     6361 gatgaaaagg ggatacctaa aaaggctgaa gaaagatttg aaatagcaaa aaggatagtc
     6421 aatagagcag tttcttgtgg gataaaacca aaagatatat ttatagactg tttatcatta
     6481 actgtttcag cacaacaaga agaagcaata gaaactataa aagctataac aatggtaaag
     6541 acactaggag taaaaactat attaggtgtt tcaaatattt catttggtct tccaaatagg
     6601 aaagcactta atgcttcttt tctaacatta gctttagggg cagggcttga tttagcaatt
     6661 ataaatccaa atgaatatag tatgatggaa gctatcaatt cttttaaaat attaaataac
     6721 acagataaag gatgtataaa ctatataaat caatatagta atataaataa ttcaaaaaaa
     6781 tcggattcat ctacaaagat tgacaaagat ttgccattgg atattttagt tgaaagagga
     6841 ttaaaagatg aagcaaaaaa tgtaacactg aatcttttaa aagagaagga tgaaaattac
     6901 atattagatg agattttaat tcctgctctt gataaagtag gaaaaagata tgatagcgga
     6961 gatatatttt taccacaatt gattcaatca gcagaaacgg taaaagtttc attaaataca
     7021 ataaaggaaa ctttattaag taaaagtagc aataatgtat ccaaaggaaa aataatagtt
     7081 gctacagtaa aaggtgatat ccatgatatt gggaaaaata tagtaaaaat aatgctagaa
     7141 aattatggat atgaggttat agatttaggt aaagatgtcc ctattgaaga agtagtggaa
     7201 gttgctaaaa agagaaatat aaaattagta ggattaagtg cacttatgac tacgactgtt
     7261 caaagtatga aagatacaat tcaggcactg agagataatg agataaatgc aaaggtattt
     7321 gtaggaggtg cagttttgac agaagaatac gctgaagaaa tgggcgctga ttattattca
     7381 aaggatgcaa agtctgctgt tgaaatagca aagttaaatt tttagcatat aaattaaaat
     7441 aaaggtttta ctaaatagga tattttatat ataatataaa tataagtttt agatacttat
     7501 gtttaaaaca ataggaatta atctataata atgagattaa ttaatataaa aaatatttat
     7561 catcaacatt aaaaagggct gggaagtctg gtataacttc cattagccct ttttagttaa
     7621 aatcatatgt taaaaagccc aattaccatt tttaaaaatc tgtatttgtt ttccatcata
     7681 agtctcacca ataatatcca tatcagcaga accaatcata aaatctacat gtgttaaact
     7741 gtcatttaca ccatgttcat ctaattcttc atcttttaaa ttgtttccac cttccagaca
     7801 agtcttataa gcagaaccaa ttgcaaagtg gcaagaagca ttttcatcat atagagtatt
     7861 ataaaaaatt atatccgtat tggatattgg tgagttgtat ggaacaagag caacttctcc
     7921 taaataatga gagccttcat ccgtttcaat aagtttttct agtgtatcta atcccttttc
     7981 agcagagaaa tctattattt taccatcttt aaatgttaag aagaaattgt caattatgtt
     8041 tccaccataa acaagaggtt ttgtactata aacagtccca tttactttaa acttatgtgg
     8101 cattccaaaa atctcttcag ttggaatatt cggattaaaa cttattccat tagggtcttt
     8161 ggatgctcca cttagccaaa catgaccctt tggcaagtct agagtcaaat cagtttttga
     8221 ggatttatat tttaaagttt taaagttatt tttatttaga aagtccattt tagatttaag
     8281 attttcatta tgttcttccc atgcttttat agggtcctga ccatctactc ttgaacattt
     8341 aaatatagca tcccacagtt tttctattgc tttatcttct gataaatttg gaaacacttt
     8401 ttttgcccaa tcttttgttg gcacagagac tatactccat ttacatttat cagttagagt
     8461 gtaagaacgc cattctttta aagcttttcc agatgccttc tgaaaatttg caactctttc
     8521 agggtcaaca tcttttaata aatctggatt ttgggcatag actgataaaa atgccccacc
     8581 ttcttttgct atatctacat attgttctgc tgtccatttg gggaactcat gaaatgcttc
     8641 atctggagca tacaagtatt taataagagt actttcttca tcagaccact ctatataaac
     8701 atttttagca ccacatttat atgcttcttt aagtgcattt cttgcaaaat cagcacattc
     8761 tattggtgac tttataagta gaatttgacc tggctgtatg ttaactccaa cttttattgc
     8821 taattcagcg tatttttcta aatttctatt taaattcata tttatcctcc tatattttta
     8881 atattgaata acttataaag tattataaca ttacttatgt ttttaatatt gaacaactta
     8941 taaaatatta taactttact tagattttgg gtcatattca aaatatttat actttataga
     9001 ttgagaaata aaaaaaatat tttaattaaa acagattatg aggataacaa acttatttaa
     9061 gcagtatgtt ttttgttgat aataatatta aagtagaaat aaaaatactt ttttagtgaa
     9121 aagctataca agaataccga aaaaaactta taatataata atgagataga tatttataaa
     9181 tgaaaaaaat ttttctggca aagcttaatt aaaaaggtaa tagctagtgt tgttatttaa
     9241 attagcatag aaaatcaaat aaaaaaatta tgctaatgta tagacattga taaggtagaa
     9301 tttaagcctt ttgaatgcta tattaaacta gagtagatat tcttatgaat ttcaaaatag
     9361 ggcaggagga aaaaggaaat aaaactataa aaaatataaa gtatttatat aaatttatag
     9421 agtttatacg tcagaagatt tcagttagga gggcataaat ggagctttat gaagccattt
     9481 tttatagaaa atctgtaaga aatttttcca gtaagaagat aaaaaagcca ttgatggaag
     9541 aaataaaaag aagttgtaaa aatttagaat acttaaatga agatttaaat attaaagcac
     9601 atgttgttga tagaggtcat ataatacatt ttttgatggg taaggaatgt aaagtaaagg
     9661 cacctcatta tatagtagtt acatcagaca aaggtgagga ctatttacag aatataggat
     9721 ttgctacaga aggtgtagtt ttgcgcctta caacattggg aatagctact tgctggttgg
     9781 aaggaaactt aaagagagaa gatatcttgg aatttgtaga tattgggaat aagaattttg
     9841 aagaagatga ggaagatgat ttagattatc tagctggagt aaaaacagaa gaagatgatg
     9901 aaaacaaaat agaaaatcct tatataataa tagcatttgg atatccagca aaaaatgaag
     9961 agttgtttag gacgagatat gctagacctg atagaaagcc agtaaaagat atgataaata
    10021 agataaatag gaatagaaag tggaaagata tcttagaatt gacaagaaga gctccatctg
    10081 taaaaaattc tcaaccgtgg tatttccata aggatgaacg aggtcttcat ttatttgaaa
    10141 agagacctaa gaaacactgt gaagatatga ataaggttag cttaggtgta gccttaagac
    10201 attttgatat agcttgtata aaaaataaaa tagatgtttc atatgaaaaa cttcctataa
    10261 ggaataaaat aggaaaatca tattttataa ctgtagtcga gcatgtgaaa ccagaagaag
    10321 aaacccaaga agaaaatgta actctagaaa aagaagaatc acaagatgaa taaaattttg
    10381 gaaggggata ttggatatgt ataagtgtgg aaaatgtaat cttagaggct gttctaatgg
    10441 aaatatggat aaattaatga agatatgtcc aactagagaa attgaactac aacaaaaagc
    10501 aaagcaactt tatgaagaag aagagaatag aaaaatagct tataatgctg gattagttga
    10561 agcagagggg tattgtcaat atactagact aaaagagatt atagagtttg ctaaaaagtg
    10621 tggttataaa aagattggca tagcattttg tattggacta aaaagagaaa tgcaaacagc
    10681 acaaaaaata tttgaacatc atgggtttga agtattttct gttatatgta aaaatggggc
    10741 aatgcctaag agtacaattg gactagaagc atctcaaaca atatcaaagt gttcagatga
    10801 ggtaatgtgc aatccaatag gacaagcttt atttttaaat gaagctaagg ttgaccttaa
    10861 tgtaattctt ggattatgtg ttggtcacga tactttagcc atgaaatata tggaagctcc
    10921 tataacaact ttagttgtca aagatagagt aactgggcat aatccagttg ctgcaatata
    10981 taatgcagaa tcatattttg agaaaaaatt gtttgataaa gatgaagaat aattcaaaga
    11041 cttattgaca taaaaagcaa ttagtggttt aattataaaa aagataaaac tttaaattat
    11101 cctatagata cataaagtta tataaataaa gaaaaatatt acagctaaat tccttaaagt
    11161 agtccagaga gactaaaggg ttatagataa tgttagtata taacctctag taaatcttct
    11221 agaggttttt gatgagtata aaataaaaat ttaaatataa taaagtcttt taaactagtc
    11281 cagagagact gggaaagata tctcatagct aatgaatgga gttatgtata gataaaatcc
    11341 ttatgcctct tttggtgtga ggattttttt atgattattt aggctttatt aaaaaggaga
    11401 tgtaaaaatg ataaatggta aagaaaaatt gatagttgca attgatactg acgaatttga
    11461 taaagctaaa gaactaatag acagattaga agatagtgta gacatattta aggtaggtct
    11521 agagcaatac gttgccacaa aaggtaaaac aatagattat cttaaagaaa aagggaaaaa
    11581 aatattttta gaccttaaat tccatgatat accaaatact atgaaaagtg cagtgagagc
    11641 tgcagtgaga gataatgttt ggttaatgac aatacatgta tcagatatgg aaggaatgag
    11701 acaatgtgca ctaattgcta gggaagaagc agaaagatta aatatagaaa agccattagt
    11761 agttggagta acagttttaa cttcattaag taaccaagat ttacaggaca taggatgtaa
    11821 tatgacaaca gaagagttag ctataaagag agctaaactg gcaaaagaat caggcataga
    11881 tggggttgtt tgttcagcgc aagaagtaga taaaatagta gaagcatgtg gagctgattt
    11941 tataacagtt tgcccaggaa taagacctaa atcagcagag gttggagacc aaaaaagagt
    12001 tgtaactcca tcagatgcaa taaaaaaagg tgctcattat ttagttgttg gaagacctat
    12061 aacaaaagca gaaaatccaa gagaatcagc tattaatatt gtaagagaaa tagaaaatgc
    12121 ttagataaaa tatttgggag gtagcttata tgaaggggaa attaatttta gaagatggaa
    12181 cagtatttga aggaaaagca tttggatatc taaaggaagc tattggagaa gttgtattta
    12241 atacttcaat ggctgggtat ggagaaatat tgacagacgc aacttattat ggacaaatag
    12301 ttactatgac ttatccactt ataggaaact atggaataaa tttagaagat gttgaatcaa
    12361 ataaggtgca agtaaaagga cttattgtaa gagaaaaaag tgataaccca aataatttta
    12421 gatgtgaaat ggatatagac acatatctaa agcaaaataa ggtaatagga cttgaaggaa
    12481 tagatacaag agcattaact aaaattatta gaaacaatgg aactaagaga ggaattataa
    12541 ctttagaaaa tgtatcatta caagatgttc agtctaaatt agaagagcat tgtaacaaag
    12601 attcagtaaa aaatgttaca agaaaagaaa ttgaaaatat aaaaggtact ggagctaaag
    12661 ttgtagttat agactttggt gttaaaaata gtgtactaga gtctcttaaa gcttgtaact
    12721 gtgatataac tatagcacca gcaatgacta cagtagaaga aattttaaat ataaatcctg
    12781 acttaatctt attatctaat ggaccaggaa atccagagga tataaatgat atagttgaaa
    12841 atataaaaaa attaataggc aaaaagccat tgacaggaat aggtttagga catcaaattt
    12901 tagcattggc attaggagga aagacttcaa agcttgtatt tggacataga ggaggtaatc
    12961 acccagttaa atgtttagaa actggaaagg tatttataac ttctcaaaat catggatata
    13021 atgtttcaga aatgccagaa aatacagagg taactcacat aaatttaaat gacaatacta
    13081 tagaaggaat gagacataag gaattaccaa tatatagtgt tcagtacatt ccagaataca
    13141 caacaaaaga agatttagat tgtatattta ataaattctt agcattaata tagatttagt
    13201 attatttaca gatagtaaaa accatttaga taaggagaaa aatttatgcc taaattagat
    13261 agtataaaga aaactttagt attagggtca ggaccaataa taataggtca agctgcagag
    13321 tttgactatt caggaacaca agcttgccag tctttgaaag aggaagggat tgaagttgta
    13381 ttaataaata gtaatccagc cactataatg actgataaag aagtggcaga taaaatatat
    13441 atagaaccat taacaataga atttatagaa aaaataatag aaaaagaaag accagacagt
    13501 ttacttgcag gtatgggtgg acaaacaggg ctaaacctag cagttgaact ttatgaaaaa
    13561 ggcatactag ataaatatgg agtaaagata ataggtactt cagtagaatc tatcaaaaaa
    13621 ggtgaagata gagatatatt tagagaagta atgaaagaaa taaatcagcc tgtaatagtt
    13681 agtgatatag ttacagattt acaagcaggt cttgattatg cacttacaat tggttatcca
    13741 gtagttgtaa gaccagcata cacactaggt ggaactggtg gtggtatagc tgataatgag
    13801 gaagaattaa gagaaatact ttcacatggt ctacaattaa gtcctgttgg acaagtatta
    13861 attgaaaaga gtataaaagg ttggaaagaa atagaatatg aggtaataag agattcaaag
    13921 ggaaattgta tagttgtatg taacatggaa aatatagacc cagtcggagt tcatacagga
    13981 gacagtatag ttgttgctcc aactcaaact ttaagtaaca aagaatgtgc aatgcttaaa
    14041 aaagcatctt tagatatatt aaatgcagta gaagttcaag gtggttgtaa cgttcaattt
    14101 gcacttaatc cacatagttt tgaatatgca gttatagaga taaatccaag agttagtaga
    14161 tcatcagctt tagcatcaaa agctacagga tatccaatag ctaaagtttc atcaaaaata
    14221 gctttaggtt atactttaga tgagatagaa aatgcagtaa ctaaaaaaac ttatgcttgt
    14281 tttgaaccaa ctatagatta tgtggtggca aaaataccaa aatggccttt tgataaattt
    14341 aaaaaagcaa atagaaaatt aggaactaag atgatggcaa ctggtgaaat tatgagtata
    14401 ggaagtaact ttgaggctgc tattcttaaa gggataagat cacttgaaac aggaaaatat
    14461 tcattagttc acacaccatc tgaggagaga agcattgagg aattaaagaa aagagttgta
    14521 gtacctgatg atgagagact atttgactta gctgaaatga taagaagagg ctataaagta
    14581 gagatgatag aacaaattac aggtatagat aaatggttta taaataaatt taaatggata
    14641 gtagagcaag aagaaaaatt aaaagttatg aaaattgaag atttaactaa agactatctt
    14701 cttgaattaa agaaaaaggg attctctgat aaaggtatat ctgacttaat gaagataagt
    14761 ccagaaaaat tatatgaatt gagaagtcta tacaatatca aaccagcata taaaatggtt
    14821 gatacttgtg caggtgagat agatgctata tctccttact attattcaac ttatgagcaa
    14881 tatgatgagg tagttgttag tgataagaaa aaggtgatag tattaggttc tggtccaata
    14941 agaataggtc aaggtataga atttgactat tgttcagttc attgtgtaaa atctttaaga
    15001 aaaatggaca tagaaactat aatcataaac aataaccctg aaactgtaag tacagacttt
    15061 gatacatcag ataagctgta ttttgaacca ttaacagaag aagaagtttt aagtataata
    15121 gaaaaagaaa agccagaagg tgtaatacta cagtttggtg ggcaaacagc tataaaattg
    15181 gctaagtttt tacatgaaaa aaatatacct atactgggaa cagattttga tgatatagat
    15241 gcagcagaag atagagaaaa atttgatgat ttactagaga gactagatat aaatagacca
    15301 aaaggaaagg gtgtatggtc acttaacgaa ggtattgaag ttgctaatga attaggttat
    15361 ccagtattag ttagaccatc ttatgtactt ggtggtcaag gtatggaaat aacttataac
    15421 gaagaaaaat tatctcaata tctaca
//
LOCUS       DAMJWP010000078        15404 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.082, whole genome shotgun sequence.
ACCESSION   DAMJWP010000078 DAMJWP010000000
VERSION     DAMJWP010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15404
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.082"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            115..1506
                     /locus_tag="PC700_001967"
     CDS             115..1506
                     /locus_tag="PC700_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDF2581278.1"
                     /translation="MSSTESVSKKSGHPKSFWLICMTILWERFSYYGLTALLVLFFTA
                     SVAQGGLGLSTQEATSMFGLFTGLIYLTPLVGGWLADKVLGQQKCISIGALLIAIGDF
                     VLFASSTSIAMVWVGLSIIILGNGFFKSSCSNIVGDIYPKTDVSRKDAAYSLFYMAVN
                     VGAFIAPIICGLLSDNVFAVRGASGDIVSYGYKMAFLICGIGMMLGMIIFTLLAPKLL
                     NEVGKYPVTKGNKGEKIEYGPLTKLEKNRIIAMVILFLFVVLFWTAYYQTQSSFMIYV
                     RDNVNRTLFGFEMPVAWLTSLNAILCVALCPVLATLWVKLSHTKRGDFSIPVKMGLGM
                     IIMGVGFIVMVFATLANGPDGMVKASILFIVLTYIFTTVGELFLSPIGLAMFNKLAPA
                     KYATLSMGAWYLTSFFASLLSGKIAGFTATLGFLQIFGGIATVLIIFGLILLGIRNVL
                     TRLMAMDLLTKEK"
     gene            1759..3552
                     /locus_tag="PC700_001968"
     CDS             1759..3552
                     /locus_tag="PC700_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2581279.1"
                     /translation="MNIKDRLSGLRKFMEEKNIDAYMIPSSDNHQSEYVGDYFKSREF
                     ISGFNGSAGTVIVTKDEAGLWTDGRYFIQAESQLEGSTIKLFKMGQEGCPTTDEYLYK
                     NIPEGGTLGFDGRVISAREGATLAEKLSKKGIKIEYQYDLIDGIWPDRPALSDSKAFL
                     LDVKYCGESFSSKLARLREKMSEKGTSTHVITTLDDIAWLFNIRGGDVKYNPVVLSYA
                     VITLKEVYLFVDESKLNEEILDELAKENVQIKPYNDVYEFVKNIDKTEKVLLDGTKLS
                     YTIYNNIPCEVEKVDEFNPVMFFKAQKNEVELENIRNSHVKDGVAFTKFMYWLKKNVG
                     KMEITEISATQKLEDLRREQEGFFEPSFNTIAAYKEHAAMMHYSATPESNYKLEAEGL
                     FLVDSGGQYYDGTTDITRTTVLGPISDELKLHFTSVARGMINLSKAKFLHGCRGYNLD
                     ILSRSCMWNMGIDYQCGTGHGIGFVLNVHEAPNGFRWRVVPERFDSAVLEEGMVTTNE
                     PGIYIEGSHGIRTENEIVVRKAEKNFYGQFMEFEVVTLAPIDLDGIVPELMNKDEKDY
                     LNWYHKLVYDKISPFLTDEEREWLKVYTRAI"
     gene            complement(3633..5015)
                     /locus_tag="PC700_001969"
     CDS             complement(3633..5015)
                     /locus_tag="PC700_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDF2581280.1"
                     /translation="MSVLKTQEKLKQPRDLYFCCSIFTFVGLAYYGMKLVLLLFLAEQ
                     VSKGGLGLTPAESASMLASFLAWTYFSPVIGGWVSDRFLGPKNCIILGMLMVSIGYFL
                     GYIANSKMDINIMIFLSGLGIGFYKGNLHTMVGNLYTNDDPRKDGAFSITYMATNLGT
                     LFGPLICGLVANEWFAMKSGLSISMYGYRYVFLFSSITVLIGLFFFIFGNRKFYKPLN
                     NDPSNGRESIRKSLEYKKMLASRPLTLIEKKRIIVILVLAFFTVFFWIAYNQASMSIA
                     LYTKEHINLTVGNFQIPTSWIDSYNGLLCVILGPISALVWVKLSQTKKGDLSVPKKMS
                     LGFILLAIAFLFMIVAVIQTGTDPNSIHKASVFWVVGFLTFQSIGEICFSPVGYGMVN
                     KLSPEKYISFLMGVWFLGKFAANKLSGYTQAIIDQLGMLQVFIVIPSFLLIFGIILLI
                     MNKKLVELSN"
     gene            5273..5620
                     /locus_tag="PC700_001970"
     CDS             5273..5620
                     /locus_tag="PC700_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDF2581281.1"
                     /translation="MIRKKSELQVEVKENLNGGIGKVKLENIIQNEELKGKGRLFKRV
                     ALPVGSSIGVHDHTTDFEVYYILKGKGKVFDNGEFVEVNEGDVVYTADGEKHSIENIG
                     EEDLEFVALVLYM"
     gene            5638..6672
                     /locus_tag="PC700_001971"
     CDS             5638..6672
                     /locus_tag="PC700_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581282.1"
                     /translation="MTGKIEEIKDKINRFCNSNLNQEYKDISFKILQNLLDNNKEIIH
                     SSRADIWSSAILNIVLEQNLLYNKKHPLHITKKEFSKQIGVSLNTINNKSSLIKDVCS
                     IDFLNQDTIAISNVEFWVKETNSKSKSVDLDLEKKKILYKRYIKLSQDANSDIESIRY
                     MEEAVNIGKSMITKEDKELGFWKGLSTRAYMIALESLARKLESINNLKESKKVLEYLI
                     EINPSDEQGIRYKLLNVLIRLNDRTSINKLFELYKEEKSATWMYSKALYYFKNKSMFL
                     ANDSIKVARSKNKYVGLYLLDWRNAFGREFVTEEEKAEAVYYYDENIVVWNEVKGSMD
                     WLLKKMLEFS"
     gene            complement(6721..7770)
                     /locus_tag="PC700_001972"
     CDS             complement(6721..7770)
                     /locus_tag="PC700_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2581283.1"
                     /translation="MEINYKKRNFKSFAKRKNIEISVQRYLIDALSYMALGLFSSLLI
                     GTIFNTLGDNFNISLFTEVISPLAKQVTGPAIAVAIAYGLQAPPLVLFSCALVGACGN
                     ELGGPVGAFVATLFAVEVGKLVSKETKIDILVTPAVTILIGVLVAELIGPAVSAFMTS
                     FGNIIMQATTMQPIIMGALVSALVGIALTLPISSAAICMMLSLGGLAGGAATVGCCCQ
                     MVGFAVMSFRENRVGGLVAQGLGTSMLQVPNIIKNWKIWIPPTVASILLGPLSTTIFK
                     MENIPIGSGMGTCGLVGQFGTVTAMQSAGKGGISMWVGILLLNFILPAIVTLLISEFM
                     RKKGLIKPEDLKLDV"
     gene            8045..9580
                     /locus_tag="PC700_001973"
     CDS             8045..9580
                     /locus_tag="PC700_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581284.1"
                     /translation="MIFVKNKKYRKIAIIISIPILLVAQYYLFKFGILRPMVKGVEIE
                     IVDGEYVKDIDKYVVKLNETVTLSTGEYIKVPSYAKNPNIWFNVLDNSGTLKIDGNKM
                     TALKVGYSSVGIMKNSRVLKKATIKVVDPEVESLDMEINGDLKHVGDSATIESTIEVD
                     YDKFKKSYKPVYKSSNENILKIKGNKVSAVGVGKATIYAKCGNKEIERTFRIQARVAE
                     INMDDIKIEVDQNEKLKPDIITSPPGLEPPKIYYELVDSKLPVERAISLSSNGNVVGL
                     REGKDRVRITCGDKSKVITVTVVKETITNDYIQNLVGSHKIVNNKVIIKLEWDYMKDV
                     FDYDIYLKDKLSGESFKKIKSITINESDLGKSNKVYATIEVDMKGNQNIDFDIYVIGK
                     KDSDTTKPSNVINITNGNGNSNNDNNIKDMRVENISANVDRDNNVIRVNWSSISHKDC
                     TYSIYVKNNTNGDGEFNLYQSDIQGNEHTISIPEGDLNLDIYVVANYNGNSSQNSDVI
                     NVR"
     gene            complement(9766..10452)
                     /locus_tag="PC700_001974"
     CDS             complement(9766..10452)
                     /locus_tag="PC700_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HDF2581285.1"
                     /translation="MKTIGLIGGMSWESTITYYQVINTVIKERLGGLHSSKCILYSVD
                     FQEIEECQSSGNWEKSAKILADAAIKLQEAGADFIVICTNTMHKVSDKIQESIHIPLL
                     HIADVTATVLREKEIKKVALLGTKYTMEQDFYKNVIINNGIEVLIPNEEDRIIVNDTI
                     FNELCLGIISESSKKAFLSIIDKLGKQGAEGVILGCTEIGLLIKQNDTSIPLFDTTVI
                     HAIEAALSSI"
     gene            10577..11959
                     /locus_tag="PC700_001975"
     CDS             10577..11959
                     /locus_tag="PC700_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2581286.1"
                     /translation="MPINSFENYPMTWKPKLDNRKPPIYKTLAMLLEEDIKRGNLNPG
                     DKLPPQRELADFLDLNLSTITRAFKLCEEKGLICAKVGKGTFISSDVNVSNTLLYQTE
                     SKDIIELGTVHPPYEQNTYIIDFIKNVLKQPEMDRFLQYMSPSGTYMQKKSIAKWIER
                     NNVYTSEENILLSTGGQNAICATLLGLFKAGDRIATDSLSFSGIKSIAKMIGIQLVPI
                     PQENNEISIEYLESYCKNENIKGIYLIPDYHNPTTHTMSDLSRKKIAKIAKQYNIIII
                     EDAINSIFRDGIQTPIFSLASDNTIYIFSTSKFLCAGLRVAFVVAPKRYIENLENALY
                     NMNLMVSPFTAEIVHRLLYSPIIDKIIEEKRDAIIERNEVADKVLSDYNLIGDKNCSF
                     RWLLLPDELDGKSFEISAKNLGVQVYCAERFSVGNSTVPKAVRICVTAPKDVEELEKG
                     LNIIKSLLKS"
     gene            12361..13629
                     /gene="eam"
                     /locus_tag="PC700_001976"
     CDS             12361..13629
                     /gene="eam"
                     /locus_tag="PC700_001976"
                     /EC_number="5.4.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 2,3-aminomutase"
                     /protein_id="HDF2581287.1"
                     /translation="MNEQTRISLERAAELKSKIDDYIQARKTINRGLEKEEEINKRKQ
                     KILSILNGTEEDWNNYKWQLSNRITDVDTLSKIITLTKKEKEYIKEVGTQFRWAISPY
                     YLSLIDPEDICDPIKLLSIPTHIELEDEQEDLDPMGEEYTNPAGCITRRYPDRLIINV
                     TNECAMYCRHCQRRRNIGQQDSHKSKAIIQESIDYIRENEEIRDVLVTGGDALTLKDD
                     YLEWILSQLKEIPHVDYVRLGTRTLVTMPQRITDEFCNMLKKYHPVYINTHFNHPMEI
                     TKESKEACEKLANAGVPLGNQAVLLNGINNDKFVMRCLNQELLKIRVKPYYIFQSKHV
                     KGTKHFNTSVDDGLEIMEYLRGYTSGMAIPTYIVNAPKGGGKTPLLPQYLVSKGTDYV
                     MLRTWEGKVIKMEDEPAVDIKKLIKEQAQD"
     gene            complement(13722..14078)
                     /locus_tag="PC700_001977"
     CDS             complement(13722..14078)
                     /locus_tag="PC700_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581288.1"
                     /translation="MKKALISLLVLSCLLLTAPVNESSYADSQKNVPVSQNTKLTQEQ
                     AKELLVKYNNEVDYIYQGNASDFEALKAKNLNGYVFLPDADGDIGYFVNENTSEIYYF
                     HPSGYLELVNQQQNNK"
     gene            complement(14229..15323)
                     /locus_tag="PC700_001978"
     CDS             complement(14229..15323)
                     /locus_tag="PC700_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDF2581289.1"
                     /translation="MQDKKIYLNIVLTVVVSYILIKVIDNYKYFFGVISLLMSLLTPF
                     IIAFVLAYIFNPLVKFLESKLNFKRIYSLLLTYGVLIVILISFILFTVPSIVNSLADL
                     VAQIPTYIDKTEQFLFDLGKSLQSVDPNTLKEYGDKIMSVMPKFSNLLIGSLGGIFST
                     TFSVGKFIVQFLLGFIICFYILLEKEKFLLFTKKVVYISLGKKYGDFIIELCQSLNLN
                     IGKYFTGKILDSFIVGVLSGIGLYFLKSEYALLFGTLMGVMNMVPYFGPVIGMAPVVI
                     INLFSNPTIALTSLIYLIIIQQVEVTFIEPKIVGGQLGLSPFFTILAVTVGGGFFGIP
                     GMILSAPVMGVIKIYFCEFVNRRHDKIQME"
BASE COUNT         5952 a         1911 c         2487 g         5054 t
ORIGIN      
        1 aggcgataat ctattgagta aacatagaaa agatgatatt ataagtatgt gctaaaatgg
       61 ttaaagcaat attaatatta aattcaacta taaaacaaaa gggggcgttt ttttatgtca
      121 agtacagaaa gcgtatctaa aaaaagtggt catccgaaga gtttttggct tatttgcatg
      181 acaattttgt gggaaagatt tagttattat ggtttaacag ctttattggt attatttttt
      241 acagcaagtg tagctcaagg tggtttaggt ctttctactc aagaagcaac atccatgttt
      301 ggcttattta caggtcttat atatcttaca ccattagtcg gtggctggtt agcagataaa
      361 gttttaggtc aacaaaaatg tatttctata ggtgcacttt taatagcaat aggagatttc
      421 gtactatttg cttcatctac atctattgcc atggtttggg taggtctatc aataattatt
      481 ttaggaaatg gtttttttaa atcaagttgt tcaaatatag ttggggatat atatcctaaa
      541 actgatgtgt caagaaaaga tgcagcatac agtttattct atatggcagt aaatgtagga
      601 gcatttatag ctccaataat atgtggattg ctttctgata atgtatttgc agtaagagga
      661 gcaagtggag atatagtatc ttatggatat aagatggcat ttttaatttg tggtataggc
      721 atgatgttag gtatgattat atttacactt cttgcaccaa aattattaaa tgaagtcgga
      781 aaatatcctg tgactaaagg taataaaggt gaaaaaattg agtatggacc acttactaag
      841 ttggagaaaa ataggataat agcaatggtt attttatttt tatttgtggt cttattctgg
      901 acagcatact atcaaacaca aagttctttt atgatttatg taagggataa tgtaaataga
      961 actctatttg gatttgaaat gccagtagca tggcttacat cactaaatgc tatattatgt
     1021 gtggctttat gcccagtatt agctacccta tgggttaaat tatctcatac caaaagaggt
     1081 gatttctcta ttccagttaa aatgggatta ggaatgataa taatgggtgt tgggtttata
     1141 gttatggtat ttgctacatt agctaatggt ccagatggaa tggtaaaggc tagtatattg
     1201 tttatagtat tgacttatat atttacaaca gtaggtgaat tattcttatc tcctatagga
     1261 cttgcaatgt tcaataaatt agctccagct aagtatgcta cattatctat gggagcctgg
     1321 tatctaacaa gtttttttgc aagtttatta tcaggaaaga ttgctggttt tacagccaca
     1381 cttggttttt tacagatatt tggaggaata gcaacagttt taataatatt tggtttaata
     1441 ttattgggaa taagaaatgt attaacaaga ttaatggcta tggatttact aacaaaagaa
     1501 aaataattca tgacgaatta ttgtaaatag atgaattact tcaaataaag gaagttacat
     1561 ttaaatataa aattaaatgt aatttcctct ttgattatga aaaaaagctt tacacaaata
     1621 gatattataa ttaaaattat aattgtgata attagagtgt ataagattta tataaattat
     1681 acaaaaaata tttatgtaat aaactttaca tacttatata tttagttatt caaactatta
     1741 tttcgaggag gtcttgttgt gaatataaaa gatagattaa gtggattaag aaaatttatg
     1801 gaagaaaaaa atatagatgc ctatatgatt ccatcttctg ataatcacca aagtgaatat
     1861 gtaggagatt acttcaaatc aagagaattt atatcagggt ttaatggttc agcagggacg
     1921 gttattgtga ctaaagacga agctgggtta tggaccgatg gaagatattt tatacaggca
     1981 gaatctcaat tagaaggaag tacaataaaa ttattcaaaa tgggacaaga aggttgccca
     2041 actacagatg aatatttata taaaaatatt cctgaaggag gaactttagg atttgatggt
     2101 agagttatat ctgctagaga aggagctaca ttagctgaaa aattatctaa aaaaggtata
     2161 aagatagaat atcagtatga tttaatagat ggtatatggc cagatagacc tgctttatca
     2221 gatagtaaag catttttatt agatgttaaa tattgtggag aaagttttag ctcaaaatta
     2281 gctagattaa gagaaaaaat gagtgaaaaa ggtacttcta ctcatgtaat aactactctt
     2341 gatgatatag catggttatt caatataaga ggtggagatg ttaaatataa tccagttgta
     2401 ctttcttatg cagtaataac tttaaaagaa gtttatttat ttgttgatga aagtaagtta
     2461 aatgaagaaa tattagatga actagctaag gaaaatgttc aaataaagcc atataatgat
     2521 gtgtatgaat ttgtaaaaaa tatagacaaa actgaaaaag ttttattaga tggaactaag
     2581 cttagttata caatttacaa taatatacct tgtgaagttg agaaagttga tgagttcaat
     2641 ccagtaatgt tttttaaagc tcaaaaaaat gaggtagaat tagaaaatat aagaaattca
     2701 catgtaaaag atggtgtggc tttcactaaa tttatgtact ggttaaagaa aaatgtaggt
     2761 aagatggaaa tcactgaaat aagtgctact caaaaattag aggacttaag aagagagcaa
     2821 gaaggtttct ttgagccaag tttcaataca attgctgcat ataaagagca tgctgcaatg
     2881 atgcactata gtgcaactcc agagtcaaat tataaattag aggcagaagg attattttta
     2941 gtagattcag gtggacaata ttatgatgga actactgata taactaggac tacagtttta
     3001 ggacctataa gtgatgaatt aaaactacac tttacttctg tagcaagagg aatgataaat
     3061 ctttctaagg ctaagttctt gcatggttgt agaggatata acttagacat actatctaga
     3121 agttgcatgt ggaatatggg aattgattat caatgtggaa ctggacatgg tataggattt
     3181 gtgctaaatg ttcacgaagc tccaaatgga tttagatgga gagtagtacc tgaaagattt
     3241 gatagtgcag ttcttgaaga aggaatggta actactaatg aaccaggtat atatatagaa
     3301 ggttctcatg gtataagaac agagaatgaa attgtagtta gaaaagctga aaagaatttc
     3361 tatggtcaat ttatggaatt tgaagttgtt acgttagctc ctattgactt agatggtata
     3421 gtaccagaat taatgaataa ggatgaaaaa gattatttaa actggtatca taagttagtt
     3481 tatgataaaa tatcaccttt cttaactgat gaagaaagag aatggttaaa agtttacact
     3541 agagctatat agtttgtact agatacaata aaaatcgagg tattataatg cctcgatttt
     3601 ttattgtata ttttattaac tttaaaaaat ttttaatttg ataattctac taatttttta
     3661 ttcattataa gtagtataat tccaaatatc aataaaaatg atggtatcac tatgaagact
     3721 tgtaacatac caagttggtc tataatagct tgcgtatagc cagataattt atttgctgca
     3781 aatttgccta aaaaccaaac acccattagg aatgaaatat acttttcagg cgaaagttta
     3841 tttaccatac catacccgac tggagaaaag caaatttctc ctatactttg gaatgttaaa
     3901 aatccaacta cccaaaatac acttgctttg tgaattgaat tagggtctgt tcctgtttgt
     3961 ataactgcta ctatcataaa caaaaatgct atagctaata gtataaaacc taagctcatt
     4021 ttttttggca cacttaaatc gcctttttta gtttgagata attttaccca tactaaagca
     4081 gagataggtc caagaataac acaaaggaga ccattgtatg aatctatcca tgaagtagga
     4141 atttgaaaat tgccaacagt aagatttatg tgttcctttg tatagagtgc aatagacata
     4201 gaggcttggt tatatgctat ccaaaaaaat actgtgaaaa aagctaacac caatattact
     4261 attattcttt ttttctcaat caaagttaaa ggtcttgaag cgagcatttt cttatattct
     4321 agtgattttc ttatagactc tctcccgtta ctagggtcat tattcaaagg tttataaaac
     4381 tttctgtttc caaatataaa gaagaataaa cctattagaa ccgtaattga tgaaaataaa
     4441 aatacatatc tatatccata catacttata gataatccgc ttttcatagc aaaccattca
     4501 ttagcaacta aaccgcatat taaagggcca aataaagtac ctagattagt agccatatac
     4561 gtaatagaga aagctccatc ttttcttggg tcatcattgg tatataagtt tccaaccata
     4621 gtatgtaagt ttcctttata aaaacctatt cctaatccag ataaaaatat catgatgttt
     4681 atatccatct tactatttgc tatgtatcct agaaaataac caattgaaac cattagcatt
     4741 ccaagtataa tacagttttt gggacctaaa aatctatcac tgacccatcc accaataact
     4801 ggagagaaat aagtccatgc taaaaaagat gcaagcattg atgcggattc tgctggtgtt
     4861 aaccctaaac cacctttgga gacttgttca gctaaaaaca ataataatac caatttcata
     4921 ccatagtatg caagtcctac aaaggtaaat attgaacagc agaaatataa atctctaggt
     4981 tgtttaagtt tttcttgcgt ttttaaaact gacatatgtc gagaacctcc agataaatag
     5041 tttaacaaaa ttttaccata ctatatgagt atatgttttt ttcatataga cgtcaacttg
     5101 taagataatt taattaagtc gaaatatatg taaaaatagt atcatttttg tgtattcagt
     5161 aatatttttt tatgataata tatgttatta ttatattaaa atacaaacat atttatggaa
     5221 aggatatgcc aatttagtag ttagattgat tgtttggcag gtgggatgag ctatgataag
     5281 aaagaaatct gaattacaag tagaagtaaa agaaaattta aatggtggta taggaaaggt
     5341 taagcttgaa aacataatac aaaatgaaga gcttaaagga aaaggaagac tatttaaaag
     5401 agtagcttta ccagtaggaa gttcaattgg tgtgcatgac catacaacag attttgaagt
     5461 atattatata ctaaagggaa aaggtaaagt atttgataat ggggaatttg tagaagtaaa
     5521 tgagggtgat gtagtgtata cagctgatgg cgaaaaacat tctatagaaa atataggaga
     5581 agaagatctt gagtttgtag cactagtttt atatatgtaa aatatggagg aggagtcatg
     5641 acaggcaaga tagaagaaat taaggacaag ataaatagat tttgcaactc caatttaaat
     5701 caagaataca aagatataag ctttaaaatt ttgcaaaacc ttttagataa taataaagag
     5761 ataattcatt cttctagagc tgatatatgg tcttcagcta tactaaatat agttttagaa
     5821 caaaatcttt tatacaataa gaagcatcca ctacatataa caaaaaaaga gttttcaaaa
     5881 caaataggtg tttctttaaa tactataaat aataaatcgt ctttaataaa agatgtttgt
     5941 agtattgact ttttaaatca agatactata gctatttcta atgtagaatt ttgggtaaaa
     6001 gagaccaatt ccaaatcaaa gtctgtagat ttagatttag aaaagaaaaa aatactatat
     6061 aaaagatata ttaagctatc acaagatgcg aatagtgata ttgaatcaat tagatatatg
     6121 gaggaagctg taaatatagg taaaagtatg attactaaag aagataaaga acttggattt
     6181 tggaaaggtt tatctaccag agcctatatg atagcacttg aatcccttgc tagaaagtta
     6241 gaaagtatta ataatctaaa ggagtccaaa aaagtattag aatatttaat agaaattaat
     6301 cctagtgatg agcagggaat aagatacaaa ttattaaatg tattaattag attaaatgat
     6361 agaacttcaa taaataaatt atttgaactt tataaagaag aaaaatctgc tacatggatg
     6421 tacagtaaag ctctttatta ctttaaaaat aaaagtatgt ttttggcaaa tgattctatt
     6481 aaggttgcta gaagtaaaaa taagtacgtt ggattatatt tgttagattg gcgaaatgct
     6541 tttggtagag aatttgttac agaggaagaa aaggcagaag ctgtctatta ctatgatgaa
     6601 aacatagtgg tttggaatga agttaaaggt tctatggatt ggctattaaa aaaaatgttg
     6661 gaattttcat aaaaaaaact actccttact tttatataag taaaaggagt agtttttaat
     6721 ttatacatct agttttaaat cttcaggttt tattagacct ttttttctca taaattctga
     6781 aatcaacaaa gttacaatag caggaagtat aaaattaagt aaaagtatac caacccacat
     6841 agatatacct ccttttccag cactttgcat agcagttact gttccaaatt gtccaaccaa
     6901 tccacaagta cccataccag aaccaatagg aatattctcc attttgaata ttgttgtaga
     6961 taatggtcca agtaatattg aagctacagt aggtggtatc caaattttcc aattttttat
     7021 tatattaggt acttgcaaca tagatgtccc aagaccttgt gccacaaggc caccaactct
     7081 attttctcta aagctcataa cagcaaagcc taccatttgg caacaacaac ctacagttgc
     7141 tgcaccacca gcaagaccac ctaaacttaa catcatacaa atagctgcac tacttatagg
     7201 taatgtaaga gctattccaa caagtgcaga gactaaagct cccattatta taggttgcat
     7261 agttgtagct tgcattatta tatttccaaa tgaagtcata aaagcagaaa cagctggacc
     7321 aataagttca gctacaagta ctccaattag tattgtcaca gcaggtgtaa ccaatatatc
     7381 tatttttgtt tcttttgaaa ctaattttcc aacctctaca gcaaaaagtg tagcgacaaa
     7441 tgctccaaca ggtcctccta attcattacc acaagctcca acaagtgcac aagaaaatag
     7501 tacaagtgga ggtgcttgaa gtccatacgc aatagccact gctatagctg gaccagtaac
     7561 ctgtttagca agagggctta taacttcagt aaaaagagaa atattgaaat tatctcctaa
     7621 tgtattgaaa attgttccaa ttaaaaggga cgaaaaaaga ccaagtgcca tataacttag
     7681 tgcgtcaata agataccttt gaacagaaat ttcaatattt tttcttttag caaatgattt
     7741 gaaatttctt tttttataat tgatttccaa tttaaaatcc tcctcatata atataagtat
     7801 aatttctttt taattaatta tataactttt cagctgacaa gacaaatgtt atttcactaa
     7861 aattagacta ttgtaatagt aagaaattaa cattaatttt agttttaaat tttaaattta
     7921 tggtttataa ttagtatagg gatattctga aatataataa ttttccagtt tatcccaata
     7981 tttaacttag gtagattgct tacaaaacaa atgattatat attaggctaa aaggaagtgg
     8041 atagatgata tttgtaaaaa ataagaaata cagaaaaatt gccataatta tatctattcc
     8101 tatattgctt gttgcgcagt actacctttt taaatttgga atacttagac caatggtaaa
     8161 gggagtagaa attgaaattg tagatgggga atatgttaaa gatatagata aatatgtagt
     8221 aaaattaaat gaaacagtta ctttgtctac aggtgaatat attaaggttc cttcgtatgc
     8281 taaaaatcca aatatttggt ttaatgtatt ggataactca gggactttaa aaatagatgg
     8341 aaataaaatg acagcattaa aggttgggta ttcttctgtg ggtatcatga aaaattcaag
     8401 agtattaaaa aaggctacta taaaagtagt agacccagag gtagaatcac tagatatgga
     8461 gattaatggg gatttaaaac atgttggtga tagtgctact atagaaagta caatagaagt
     8521 tgattatgat aagtttaaaa aatcatataa gccagtttat aagagtagca atgaaaatat
     8581 tttaaaaatc aaaggaaata aagtaagtgc agttggagtt ggtaaagcta caatatatgc
     8641 taagtgtgga aataaagaaa ttgagagaac atttaggatt caagctagag ttgctgaaat
     8701 aaatatggat gatataaaaa tagaagttga ccaaaatgaa aaattgaaac cagatataat
     8761 aacttctcca cctggtttag aaccaccaaa aatatattat gagttagtag attcaaaatt
     8821 acctgtagaa agagcaataa gtttaagtag taatggaaat gttgtaggtc ttagagaagg
     8881 taaagatagg gtgagaataa cttgtggaga taaatctaaa gtaataacag taacagtagt
     8941 caaagaaact ataacgaatg actatataca aaacttggtt ggttcacata aaatagtaaa
     9001 caataaagtt attattaaac ttgaatggga ttatatgaaa gatgtctttg attatgatat
     9061 atatcttaaa gataagttgt caggagaatc atttaagaaa attaaatcta taactataaa
     9121 tgaaagtgat ttaggtaagt ctaacaaggt atatgcaact atagaagtag atatgaaggg
     9181 taatcaaaat atagattttg atatttatgt catagggaaa aaggattcag atacaactaa
     9241 gccaagtaat gtaataaata ttactaatgg aaatggaaat tcaaataatg ataataatat
     9301 taaagatatg agggtagaaa atatatcagc taatgttgat agagataata atgtcattag
     9361 agttaactgg agtagtatat ctcataagga ctgtacttac agtatatatg taaaaaataa
     9421 tactaatgga gatggagaat ttaatctata tcaaagtgat atacagggaa atgagcatac
     9481 tataagtatt cctgaaggag atttaaatct agatatttat gttgttgcaa attataatgg
     9541 aaatagctct caaaatagtg atgttataaa tgtaagatag atttgtatac aaattgaaag
     9601 tgttgagttt atttaaatat taaattaaga aaaaggatag tatagggtaa ttatactatc
     9661 ctttttttgt tacaatatat aggtttaaaa tctctagtaa tataaattat aaaaagtctt
     9721 atttacacaa atctatagat atttttttta ttaatcaata ttgtcttaaa ttgaacttag
     9781 tgcggcttct attgcatgta ttactgtcgt atcaaatagt ggaatagaag tgtcattttg
     9841 tttaattaaa agacctattt ctgtacatcc taatataaca ccctctgcac cttgtttgcc
     9901 aagcttatct ataatagata agaaagcttt ttttgaagat tcagatataa ttcctaagca
     9961 aagttcgtta aatattgtgt catttactat tatacggtct tcttcatttg gaattaagac
    10021 ttcaattcca ttattaataa ttacattttt ataaaaatct tgttccattg tatatttagt
    10081 acctaataag gccacttttt ttatttcttt ttcccttaat actgtagcag ttacatctgc
    10141 tatgtgaagt aatggaatat gtatactttc ttgtatttta tctgatacct tatgcatagt
    10201 attagtacag ataacaataa aatctgcccc tgcctcttgt aatttaattg cagcatcagc
    10261 taaaattttg gcgctttttt cccaatttcc actagattgg cattcttcaa tttcttgaaa
    10321 atctacactg taaagtatac atttagaaga gtgtagtcca cctaatcttt cttttattac
    10381 tgtattaata acttgataat atgtaatggt actttcccaa ctcataccac caattaagcc
    10441 tatagtcttc atattatacc tctcttattt atttattttg aaatagctat ttacaaatac
    10501 aagtatagca tctaaaatag aagcatacaa taataaaatt gtatggagta caattataat
    10561 taagaggagt aattatatgc caataaattc ttttgaaaac tatcctatga catggaaacc
    10621 aaaattagat aaccgaaaac caccaatata taaaacttta gcaatgttat tagaagaaga
    10681 tattaaaaga ggaaatttaa atcctggaga taaactgcct ccacaacggg aattagcaga
    10741 ttttctagat ttaaacttaa gtacaataac acgagcattt aaactttgcg aagaaaaggg
    10801 actaatatgt gcaaaagtgg gaaagggaac attcatttct tctgatgtaa atgtaagtaa
    10861 tacattatta tatcaaacgg aatcaaaaga cataattgaa ttagggacag ttcatcctcc
    10921 atatgagcaa aatacatata ttattgactt tattaaaaat gtactaaaac aaccagaaat
    10981 ggataggttt ttacaataca tgtctccatc tggcacatat atgcaaaaaa agtcaattgc
    11041 aaaatggata gaaagaaata atgtatacac cagtgaagaa aatatattat tatctacagg
    11101 agggcaaaat gctatatgtg caactttact aggattattt aaagctggtg atagaattgc
    11161 tacagattct ttaagttttt ctggaattaa gtcaatagct aaaatgatag gtattcaatt
    11221 agtcccaatt cctcaagaaa ataatgaaat atcgattgaa tatcttgaaa gttattgtaa
    11281 aaatgaaaat ataaaaggca tatatcttat accagattat cataatccaa cgacacatac
    11341 tatgtctgat ttatctcgta aaaagatagc caagatagca aagcaatata atataataat
    11401 tattgaagat gctataaata gcatttttag agacggtata caaactccca tattttcttt
    11461 agctagtgat aatactatat atatttttag tacatctaaa tttttatgtg ctggtcttag
    11521 agtagcattt gtagttgctc caaaacgata tatagaaaat ttagaaaatg cactatataa
    11581 tatgaatcta atggtttctc catttacagc agaaatagta catcgtttat tatattctcc
    11641 tattatagat aaaattatag aagagaaaag agatgcaatt atagaaagaa atgaagttgc
    11701 tgataaagtt ttgtcagatt ataatttgat tggtgataag aattgcagtt ttcgttggtt
    11761 acttcttcct gatgaattgg atggaaaatc atttgaaatt tctgcaaaaa atctaggagt
    11821 tcaagtatat tgtgctgaac gattttcagt aggaaactct acagttccaa aagcagttcg
    11881 tatttgtgta acagctccaa aagatgtaga agaattagaa aaagggctta atataataaa
    11941 gtcacttctt aagagttaaa tttattttac tattatttaa ttaaataaat aaaaaggcgg
    12001 agaaatttat tataataaac ctccgctttt attatttaat gaaatacttt actttttatt
    12061 aaaaaattta attttaataa tcttgaaaat ataatcgttt tatggtaaaa taattcttaa
    12121 aaatagaaaa aattaatcat ttgttaaaaa aaatgcataa tttattgtaa tttattgacg
    12181 aaggttgaaa aatattcata aatgtgcgaa ataacagaaa aaatgcaaaa attaattcaa
    12241 tttcatttgt tataaaatct atatggcaaa attaaatctg cctatcagaa tgatataaac
    12301 acatcagaaa ggaggacgct atcaaataat acaagtagtt aactttgata gcaaaaaatt
    12361 atgaatgaac aaactagaat atccttagag agagctgctg aattaaaatc aaaaattgat
    12421 gattatattc aggctagaaa aacgattaac agaggtcttg aaaaagaaga agagataaat
    12481 aaacgaaaac agaaaatatt aagtatctta aatggaactg aagaggattg gaataactac
    12541 aaatggcaat tatcaaatag aataacagat gtagatactt tatcaaaaat tataactcta
    12601 actaaaaaag aaaaagaata tataaaagag gttggtactc aatttagatg ggcaatatct
    12661 ccatattatt tgagtcttat agacccagaa gatatatgtg acccaataaa attactgtct
    12721 ataccaacac atatagagtt ggaagatgag caagaagatt tggacccaat gggagaagag
    12781 tatacaaacc cagcaggatg tataactaga agatacccag atagattaat aataaatgta
    12841 acaaatgagt gtgctatgta ttgtagacac tgtcagagaa gaagaaatat tggacaacaa
    12901 gattctcata agtcaaaagc tattatccaa gaatctatag actatatcag agaaaatgaa
    12961 gaaataagag atgtactagt aactggtgga gatgctctta cattaaaaga tgattattta
    13021 gagtggattc ttagccaact taaagagata ccacatgttg attatgttag attaggtact
    13081 aggactcttg ttacaatgcc acaaagaatt acagatgaat tttgcaatat gctaaaaaaa
    13141 tatcaccctg tatatataaa tactcatttt aatcatccaa tggaaataac taaggaatct
    13201 aaagaagctt gtgaaaagtt agcaaatgca ggagttccat taggaaatca ggcagtatta
    13261 ttaaatggaa taaataatga taaatttgta atgagatgtt taaatcaaga attactaaaa
    13321 ataagagtaa aaccttatta tatattccaa agtaaacatg taaagggaac aaaacatttc
    13381 aatacatcag tagatgatgg tcttgaaatc atggagtatt taagaggata tacatcagga
    13441 atggctatac caacatatat agtaaatgcg ccaaaaggag gaggaaagac tcctttgctt
    13501 ccgcaatacc ttgtatcaaa aggaacagat tacgttatgc ttagaacatg ggaaggaaaa
    13561 gttataaaaa tggaagatga acctgctgta gatataaaga aacttataaa agagcaagca
    13621 caggattaaa aaagtattta aagtttataa aaagaaacaa ctttaaaata aaaagaaaat
    13681 ggatgtcaaa tttatatcaa tttgcatcca ttttttatta gttatttatt attttgttgt
    13741 tgattaacta attcaagata tccacttggg tggaagtaat atatctcaga agtattttca
    13801 tttacaaagt aaccaatatc accatctgca tctggtaaaa atacataacc attaagattt
    13861 ttagctttta atgcttcaaa atcactagca tttccttgat atatgtaatc aacttcattg
    13921 ttgtatttta ctaaaagttc tttagcttgt tcttgagtca atttagtatt ttggcttaca
    13981 ggaacattct tttgagaatc tgcataagaa gattcattta ctggtgcagt taataacaaa
    14041 caagataaaa ctaataaaga tataagtgct tttttcatac taaaaccctc tttcatgttt
    14101 aattatataa gattcattac ttaataatat tgtatgtttc ctataattaa atattaccat
    14161 taaagagaat aatattacag ttacacagct gtaacttttg attacaaaat cgtaattaaa
    14221 ttgtattttt attccatttg tattttatca tgtctacgat ttacaaactc acagaagtat
    14281 atctttataa cgcccatcac aggagcagat agaatcatac caggtatacc aaagaatcct
    14341 ccaccaacag taactgctag gattgtaaag aatgggctaa gacctagttg tccacctacg
    14401 atttttggct ctataaatgt aacttcaact tgttgaatta ttattaagta aattaaagaa
    14461 gttaaagcaa ttgttggatt ggagaataaa ttaattatta caactggtgc catacctatt
    14521 actggaccaa aatatggaac catattcatg acacccataa gtgtaccaaa taatagtgca
    14581 tattcagatt ttaaaaagta aagaccaatt ccagaaagta ctccaactat aaaagaatct
    14641 aaaatcttac ctgtaaagta tttaccaata tttaaattta agctttgaca tagttctatt
    14701 ataaagtctc catatttttt acctagggaa atataaacta ctttttttgt aaacagtaag
    14761 aatttttctt tttctaataa tatatagaaa caaattataa aacctaaaag aaattgtact
    14821 ataaattttc caactgaaaa tgtagtgcta aatatcccac caagtgaacc aataagcaag
    14881 ttactaaatt taggcattac actcataatt ttgtcaccat actcttttaa tgtattaggg
    14941 tcaactgatt gtaaactctt acctaagtcg aataagaact gctctgtttt atctatgtat
    15001 gtaggaattt gagcaactaa atcagctaaa ctatttacta tggatggaac tgtaaataat
    15061 ataaaggaaa ttagtataac tatgagtaca ccataagtaa gaagtaacga atatattctc
    15121 ttaaaattta atttactttc taagaacttt actagtgggt tgaatatata tgctaataca
    15181 aatgctatta taaatggtgt tagtagtgac attaataaac ttatcactcc aaaaaaatat
    15241 ttgtaattat caataacttt aatcaagata tatgaaacaa ctacagtgag tacaatattt
    15301 agataaattt ttttatcttg caaaataaca caacctttct tatatttatt aaatttagat
    15361 atacttaaat ctaagttaaa gttaatattt atagcttatt atac
//
LOCUS       DAMJWP010000079        15075 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.083, whole genome shotgun sequence.
ACCESSION   DAMJWP010000079 DAMJWP010000000
VERSION     DAMJWP010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15075
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.083"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(<1..970)
                     /locus_tag="PC700_001979"
     CDS             complement(<1..970)
                     /locus_tag="PC700_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HDF2581290.1"
                     /translation="MIFDGHSDTWADVTIRRLNGEKDVFRRIHLDNFKRANIEGAIFV
                     IWIDPPYDKEPEKRVKQIISSIKDEIEDMKDVVNIVKKYSDIDKGISENKINILIGLE
                     GLSHIGRNIDLLDYFYKEVGARHASLTWNEQNELATGCLGDENRGLTEIGKKAVNKME
                     KLGMIVDVSHLNEKSFWDLMSVASKPVIASHSNARSLCDAKRNLTDNQLKEISNTGGL
                     VGINSFRGFTNSNPKKQDLIHLVDHIDYMVNLIGIEHVSFGFDFSDYLGKETIGSFTG
                     NASVPSVDGLESTHTAQNIVVELENRGYKKKDIELISSGNFYRLFKD"
     gene            complement(1027..2229)
                     /locus_tag="PC700_001980"
     CDS             complement(1027..2229)
                     /locus_tag="PC700_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 domain-containing protein"
                     /protein_id="HDF2581291.1"
                     /translation="MKSFVSPDNIWVLWAVVTGWAAFSIYLEQKYNWASKVSGAIIAL
                     VGAMLLSNLNIIPVESVVYDQVWGYVVPLAIALLLYQCNIKKIWKESGRLLIIFLVGS
                     VGTVLGAMIGFLALKNVVPDLNIVAAMMTGSYIGGNVNFAAMSGAFDAPGELVSATVV
                     ADNLLMALYFFVLIAIPSIGFFRKHFKHPHVDEMESIGITEGETVAANYWGRKEISLK
                     DIAIAIGSAFIIVAVSVELSTWFKAIIPLSNPFLAMLNTLFGNQYLIITTLTMLFATF
                     MPNFFGDIKGAQELGTFLIYIFFVVVGVPASITLIIQKSPLLLLYCGIMVLINMLVTF
                     IVAKIFKFSLEEAILASNANIGGPTTAAAMAISKGWSKLVGPILIVGTFGYIVGNYFG
                     LLVGNILI"
     gene            2424..2978
                     /locus_tag="PC700_001981"
     CDS             2424..2978
                     /locus_tag="PC700_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDF2581292.1"
                     /translation="MNIDVGSKIRSLRKSKNISISTLAKNSDLSTGLISQIERNMVVP
                     SIVAMWKISKALDINIGYFFEEIGREDSDIVVKKNNRKKIVTNDSTKFYELLVPNLSG
                     KKIEFILVTLDGHTQKNSEFVTHEGEECGYIIKGKMKITLGNKEYLLEEGDSFYFNST
                     IPHVYENYDDEVCISVWAMTPPSF"
     gene            complement(3093..3884)
                     /locus_tag="PC700_001982"
     CDS             complement(3093..3884)
                     /locus_tag="PC700_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HDF2581293.1"
                     /translation="MIVFSDLDRSIIYSNKFLNADSKYANIEIYREKEISYISLDTIN
                     LIKQIQYYGMFIPTTTRTVEQFKRIEFNKYGIYFPWSITSNGGVILKDNEILKSWSEK
                     IDKLKPDYEPIESMIHKFKDYLNVDGITNFKVAEDTFFYIVVDFSRFNLDSIKEYTNI
                     LESKNWKFYVSGRKIYFIPKEISKENAIKYLTKELGIEYFYAVGDSIMDYGMLNISNK
                     SYVLKHGDINKNEIENSFISSSFKGMSGTEEILSNILDENCLLNI"
     gene            complement(3912..5000)
                     /locus_tag="PC700_001983"
     CDS             complement(3912..5000)
                     /locus_tag="PC700_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HDF2581294.1"
                     /translation="MRDFSTYSKDDVIFLLKDISNLIEEEGNKERELKIQNGRHYSEM
                     IPIEYEVSEDYLNLYHEKLRENKDKLAFSIGVMCEKIIKKNGNDVVLVSLARAGTPIG
                     ILAKRYIRSKYNVSLPHYTISIIRDKGIDMNAIEYIVKNHPSSKIQFLDGWTGKGTIS
                     KELEKACDELNVKFKKHFDSTLAVLADPAGYSGLYGIREDFLIPSACLNSTVSGLVSR
                     TVLREDLIGKNDFHGAKFYRHLKDKDESMNYIETIEECFKNQFKNISDEVENWESDII
                     TRDGYFDVLNIKEKYNITDINFIKPGVGETTRVLLRRVPYKILVKDLNDKSLDHIFIL
                     AKEKNVEVEQMDLKAYKCCGIIKNMKDI"
     gene            complement(5054..6292)
                     /locus_tag="PC700_001984"
     CDS             complement(5054..6292)
                     /locus_tag="PC700_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HDF2581295.1"
                     /translation="MKNCLNFNCEIDIIDNPLNLELNDFLDVGVRNNSKRRFLFISKK
                     LGKHLACKPSEMDNLGKLMATIYNEKNKEESNGTVIAFAETGTAVGHSFFDYLCGDYE
                     FIHTTREQFDELENLEFLEEHSHATDQNLYFGMLNNFKFGDEIILVDDEITTANTCMN
                     IIRKIQSIYPKKKYTICSILNWVSDENLSKVTRLERELNCKIDFVYLFKGNFKFYIDE
                     DIISNYNDNYSITKLETISSDKVNLVVNHIYVDLEDYIGNKKYVKYTGRFGINRREQF
                     RLKEIVKRESKKITMASKESLKNEKEKILFLGTEEFMYIPMLFAKEKENQYDIYYHST
                     TRSPIINIDKKHYPIKSKFMLKSFYNIDIQNYIYNIDKHNFSKCYLFVELNKSENSYL
                     EFIDIIKKTSIKELTIVCCS"
     gene            complement(6270..7346)
                     /locus_tag="PC700_001985"
     CDS             complement(6270..7346)
                     /locus_tag="PC700_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HDF2581296.1"
                     /translation="MKYFDYICKDDLERIFLKEPEDFSAKTEKDVLKYALGAFLYVPA
                     TQYNMIYKSIIGDVKGVRPLAICLEDAVGVNGELEAIENLRLILKNISNESITNKDGI
                     PLIFVRIKDVEQLLRIKEIIIKNSHSITGILIPKANSELIENCIEALYSMNLQDMYVI
                     PIIETREFIYNEKKELSFTNLYNAILRHKSRILSIRVGITDILGMYGIRRDKNFSIYN
                     NLICSSFILDIITYLQRPELDIPISGGVSEFFDMTNKDIRNKYIEEILLDKYHGLIGK
                     TVIHPMQIQIVQALSTVSYEDFTDALDILDSTDSKYGVSKGVLGERMNETNPHFLWAK
                     KTLILSKIYGVLNKGVDYEELLKF"
     gene            complement(7410..8546)
                     /locus_tag="PC700_001986"
     CDS             complement(7410..8546)
                     /locus_tag="PC700_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDF2581297.1"
                     /translation="MGININSFSKDKNSVEVTENLPTAVQEENFDIMEYTNNKKNELR
                     KSKEVEALTSLIEVENPDTILQFGRKASEGVARVSDSLLNTIKLNRNEENSKMLVHLT
                     KIMDKFDLDDFQETKEPNFVQKLFKKANNAIEMMFQKYETLGGEVEKIQIELEQYERD
                     IALSNKQIGAMLNENFEFYNELQKYIVAGEMAIEEMDNEILPFFKQKSETSGDQMDVV
                     NYQELLKVYDMLNQRVYDLRIAENIAIQTIPMLRGMQHNNYGLIRKINSAFVVTLPVF
                     KQCLSQAILLKKQELQAKSLKALDDKTNELLLRNAQNVSTQSAQIARMAGTSSVQIET
                     LEKMYNTIKSGIDETMRIEENNRALIKDNTKRLEELNTTIIYNK"
     gene            complement(8547..10109)
                     /locus_tag="PC700_001987"
     CDS             complement(8547..10109)
                     /locus_tag="PC700_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceG family protein"
                     /protein_id="HDF2581298.1"
                     /translation="MGDSKVFEKIFSSQSDRGNYTPSKGYLSYFISYIGLEDEVLYNL
                     EIFKTKQNIDSKKDIALFTDVIANPSDFDIINYFKSGLQKYRTSMEDVDINILGFEEI
                     DYKIKQAMDRVLKEEEKEFTNDRVKQNFIVKIMAWIKIYIGALDINKNEAPKVIFYGD
                     IKKHEVYLLLILYLAGFDVLYLNPNSKSNIDILKSERYNIEFEEANIIEEKISFEERV
                     ILGEKIDKSSVKKAFTVGAEASKRISEELLNDAGFIKPWQLQDRKIKNLLLSSTVDEI
                     SIYWNQPLKLRPGFKFNDAIVEAPNFLSKINGIYNDKNEYIKFLDLLRDSESSTFIEF
                     NGDVDRFSKAFTREAFSLSFLLDSKGVIDKNSVLNNKDYSISTLALNQQIMILEKVEE
                     LLEGSMFLNGLSGEDKIKGLFTVLHMDKKFVHMMNNFDYSLINPKLIIYMYKSIVFDK
                     EIVFLMLLLSKIGFDIIILCPGGENNIENVINNQLIDIHRLDKMVYDLKLNSLENDIP
                     LLKKIFGKRRRF"
     gene            complement(10196..12955)
                     /locus_tag="PC700_001988"
     CDS             complement(10196..12955)
                     /locus_tag="PC700_001988"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HDF2581299.1"
                     /translation="MEFNNDIKYYNVDKEEVTKDLSVNPEKGLSESEVKTRREKYGLN
                     EFTPKEEGSFWDDLKESLSEPMIVILIIAAVVSAVIGETHDAIGIVGAIAIGIAIGMI
                     TEGRSKKAAEALSKLTENIEVKVLRDGEVHQISKSELVPGDIVYIETGDMVPADGRLI
                     ESINLKIREDMLTGESDDVSKKCDVVVSMEEIESKGAVVVQEPIPAKQINMVFGGTLV
                     AYGRGALVVTSIGDNSEMGKIAQNLSETDEQTPLQIKLGNLGGLIAKVSSAVAGLLFI
                     FMVFQMISKNVLNVDMSGILPFLESIDPVKTAFTVCVALIVAAVPEGLPTMINMTLAI
                     TMQKMAKINALVTKKEACETIGSVSVICSDKTGTLTENRMTVEVAYVDGRYIESSEEE
                     INSYFEENCMINSTADVEHNDGDIKYLGSATECALLLYYKNVDYRQVRKNSNIVAQNP
                     FTSDSKRMTSVIGQDNHHVLLSKGAPEVLLELCSHIQRGKDIVPLTENIKHEILEEIK
                     KLQIKSMRTLGFAYKEISQAEEEAAVTAESDAMNVSAMENNLVFSGFVGIRDPLRKDV
                     IESVNTANKAGVSVKMLTGDNINTARAIGEELGLLKNNMRVVEASYIDTLDDEELKQE
                     IQSISIVARSKPDSKMRIVSALQKSGDVVAVTGDGINDAPALSKADVGIAMGISGTEV
                     SKNAADIILTDDSFSTIVKGIKWGRGIYDNFQRFVQFQLTVNVIAFLVAIISQVMGQE
                     MPFTTIQLLWVNIIMDGPPALALGLEPVRDHVLNRKPVDRKANIISKSMVYTIVLNAF
                     YITAILMLQSTFNFLGVDEAHKGTVMFALFAFSALFNAFNCREFNADSIFPNFTHNKL
                     ALQIIVLTGIAQVIFTQLFQDFFNSVAMDFMTWVKVLCVAASVIVVNEVVKCIIRLVK
                     PKKTA"
     gene            complement(12945..13574)
                     /locus_tag="PC700_001989"
     CDS             complement(12945..13574)
                     /locus_tag="PC700_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2581300.1"
                     /translation="MSINLSKGDKIDLKKSNPGLSNILVGLGWDPVQQSGGGFFKSLF
                     GGGQADIDCDASVFMLNQEGKLSGIKDLIYFGNLKSACKSVLHTGDNLTGEGTGDDEQ
                     ILVNLDKVPSNIHKLLFVVNIYNCVDRKQHFGMIENAYIRVEDQGNKKEIAKYNLSDN
                     YSEKTTLIVGAIYRKDGSWQFKAIGEGTKDAGLKEVMQNLDRIECAYGI"
     gene            complement(13660..14238)
                     /locus_tag="PC700_001990"
     CDS             complement(13660..14238)
                     /locus_tag="PC700_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2581301.1"
                     /translation="MAIVLKKGQKIDLTKGNPGLKNIKLGLGWDTNSFDSGYDYDLDV
                     SVFMVGESQRVEKDEDFIFYNNLRHPSGAVEHLGDNRTGEGDGDDEEILVNLETMPKH
                     IQKIAVTVTIYEAAERRQNFGQVSNSYIRVLNSENDEEILRYDLGEEFSIETAITVCE
                     IYKYNGEWKFSAVGAGFEGGLEALCRNYGLNV"
     gene            complement(14277..14855)
                     /locus_tag="PC700_001991"
     CDS             complement(14277..14855)
                     /locus_tag="PC700_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2581302.1"
                     /translation="MGITLSKGQKVDLTKGNPGLKNILVGLGWDTNKYDGGFDFDLDT
                     AAFLTGASGSVTNDGDFVFYNNLKHTSGAVEHLGDNRTGEGDGDDEQIVVDLSKIPGE
                     ISKISFTVTIHDATERRQNFGQVSNSYIRIVDKDTNEELIKYELGEDFSIETAIVVAE
                     IYKHNGEWKFNALGSGFEGGLAALCGNFGINL"
BASE COUNT         4841 a         2744 c         1561 g         5929 t
ORIGIN      
        1 tatctttaaa gagcctataa aaattaccac tagagattaa ctctatatct ttctttttat
       61 aacctctatt ttcaagctct actactatgt tctgtgctgt atgtgtgctt tcaagaccat
      121 ctacacttgg cacactagca tttccagtaa aagaacctat agtctctttc cctaagtaat
      181 cactaaaatc aaaaccaaaa gatacatgtt ctatacctat aagatttacc atatagtcta
      241 tatggtcaac caaatgtatt aaatcttgct tcttaggatt actattagta aatcctctaa
      301 aactatttat tccaactaat ccacctgtat ttgaaatttc ttttaattga ttatctgtaa
      361 gatttctttt agcatcacat aaactcctag cattagaatg agaagctata actggcttac
      421 ttgcaacaga cattaaatcc caaaatgatt tttcatttaa atgtgacaca tctactatca
      481 tgcccaattt ttccatttta ttgactgctt ttttacctat ttcagttaaa ccacgatttt
      541 catcccctaa acatcctgta gcaagttcat tctgttcatt ccaagttaat gatgcatgtc
      601 ttgcaccaac ttctttataa aaataatcta gtaaatctat atttcttcca atatggctta
      661 atccttcaag accaattaaa atatttattt tgttttcact tataccttta tctatatctg
      721 aatatttttt tactatgttt acaacatctt tcatatcttc aatttcatct tttatagagc
      781 ttataatttg tttaactctt ttttcaggct ctttatcata tggtgggtct atccatatta
      841 caaaaatagc tccttctata tttgctcttt taaaattatc aagatggatt cttctaaata
      901 catctttttc cccgttaagc cttcttattg ttacatctgc ccaagtatct gaatgtccat
      961 cgaaaatcat aacaacctcc aattaaataa aaatctttct aaaaaacaac ttcttttcac
     1021 gtaaaattat atcaaaatat ttcctactaa aagaccaaaa taattaccaa ctatgtatcc
     1081 aaatgttcca actataagta ttggccctac tagcttagac catccttttg atattgccat
     1141 agctgctgct gttgtaggac caccaatatt tgcattagat gcaagaatag cttcttctaa
     1201 actaaactta aatatttttg caactataaa tgtaactagc atgtttatta gtaccattat
     1261 tccgcagtat aaaagtagta atggtgattt ttgaattatt aatgttattg aagcaggaac
     1321 acctactaca acaaagaata tatatattaa aaatgtaccc aattcctgtg ctccttttat
     1381 atcaccaaag aaattaggca taaatgtggc aaacaacata gtcaaagtag ttattattag
     1441 atattgattt ccaaataaag tatttaacat tgctaaaaaa ggatttgata atggtattat
     1501 agctttaaac caagttgata gttctacaga tactgcgaca attataaagg cgctaccaat
     1561 ggctattgct atatctttta aagatatttc ttttcttccc caataatttg ctgctactgt
     1621 ttctccttca gttataccta tactttccat ttcatctaca tgtggatgtt taaaatgttt
     1681 tctaaaaaat ccaattgatg gtatagctat taagacaaag aaatataatg ccattaaaag
     1741 attatctgct acaactgttg ctgatactaa ttctcctggc gcatcaaacg cccctgacat
     1801 agcagcaaag tttacatttc cacctatata agaacctgtc atcatagctg caacaatatt
     1861 taaatctggt actacatttt ttaatgctaa aaatccaatc atagctccaa gtacagttcc
     1921 tacagaacct acaagaaata taatgagcaa tcttccactt tctttccata tctttttaat
     1981 attacattga tataacaata aagctatggc taaaggaaca acatatcccc atacttggtc
     2041 atatacaaca gactctactg gtataatatt tagatttgat aataacattg ctcctacaag
     2101 agctattatt gctcccgaca cttttgatgc ccaattgtac ttttgttcca agtaaatact
     2161 aaatgctgcc catccagtta caactgccca taatacccaa atattatcag gacttacaaa
     2221 actcttcatc ttaattacct ccatttcatt aaattaaaat atcattatca atattcaata
     2281 tattgaatat taatactatt ataatgcatc ttttcagaaa attcaatata acataaaaaa
     2341 tacatgcatt ataaaacttt tttttatttt attaatcttt ataaattata tgttataatc
     2401 atgtctgagg gggtttgtat ctaatgaata tagatgtcgg aagcaaaatt agatctctta
     2461 gaaaatctaa aaatataagt atctctactt tagcaaaaaa ttcagattta agtacaggct
     2521 taataagtca aattgagcgt aatatggttg ttccatctat tgtagcaatg tggaaaatat
     2581 caaaagctct tgatataaat ataggttatt tttttgaaga gattggaaga gaggactctg
     2641 atatagtagt aaagaaaaat aatcgaaaaa aaatagtaac taatgattct acaaaatttt
     2701 atgaacttct tgttcctaat ttatctggga aaaaaataga atttatactt gttacgctag
     2761 atggtcatac acaaaaaaac agtgaatttg tgacacacga aggagaagaa tgtggctata
     2821 taataaaagg taaaatgaag ataacacttg gaaataagga atatctattg gaggaaggag
     2881 atagttttta ttttaacagt actataccac atgtgtatga aaattatgat gatgaggtat
     2941 gtatctcagt ctgggcaatg actccacctt cattttaaca tattgagttc ttacttaaat
     3001 ataaattgat aaaaatctga aataaattag tatgataaac tttactataa tttatttcag
     3061 atttttctag tttattattt atatatcagt cattatatat taagtaggca gttctcgtct
     3121 aaaatgttag ataatatctc ttcagttcca ctcattcctt tgaaagaact tgatataaat
     3181 gaattttcta tttcattttt atttatatca ccatgcttca atacatagga tttatttgag
     3241 atattaagca taccatagtc cataatagaa tctccaactg cataaaaata ttctattcct
     3301 aattctttag tcaagtattt tatagcattt tcttttgaaa tttcttttgg tataaaatat
     3361 atttttctgc cactcacata aaacttccaa ttttttgatt ccaatatatt tgtatattct
     3421 ttgattgaat ccaaattaaa tcttgagaaa tcaacaacta tataaaaaaa tgtatcttct
     3481 gcaactttaa aattagttat tccatctaca ttcaaatagt ctttaaattt atgaatcata
     3541 ctttcaatgg gctcataatc aggttttagt ttatctattt tttcactcca tgatttcaat
     3601 atttcattat ctttaagtat aactcctcca tttgaagtta tactccaagg aaaataaatc
     3661 ccatatttat taaattctat acgcttaaat tgctccactg ttctagtagt tgtaggtata
     3721 aacataccat aatattgtat ttgctttatt aaatttatag tatctaaaga tatataagat
     3781 atttcttttt ccctataaat ttctatatta gcatatttac tatcagcatt taaaaattta
     3841 ttggagtaaa tgatactcct gtctaaatct gaaaaaacaa tcattcaata ttctcctgtc
     3901 taatatattt cctaaatatc tttcatattt tttattattc cacaacattt ataggctttt
     3961 aaatccattt gttcaacttc aacatttttc tccttagcta atatgaatat atggtctaaa
     4021 gatttatcat ttaagtcttt aacaagaatc ttatacggaa ctcttctaag taaaaccctt
     4081 gtcgtttccc ctacacctgg tttgataaag ttaatatctg ttatgttata cttttctttt
     4141 atatttaaaa catcaaagta tccatctctt gttataatgt cactttccca gttttcaact
     4201 tcatcagata tattcttaaa ttgattttta aaacattctt ctatagtttc gatatagttc
     4261 atagattcat ctttatcttt taaatgtcta taaaacttag ctccatgaaa gtcattttta
     4321 ccaattaaat cctctcttag tacagttcta cttactaaac cagaaacggt tgaatttaaa
     4381 catgcacttg gtattaagaa atcttctcta attccataaa gtcctgaata tccagcaggg
     4441 tcagctaata cagcaagtgt tgagtcaaaa tgttttttaa atttaacatt aagttcatca
     4501 catgcttttt caagttcttt tgatatagtt cccttaccag tccatccatc taaaaattga
     4561 atttttgagc tcggatgatt ctttactata tattcaattg cattcatatc tattccctta
     4621 tctcttatta tagagattgt atagtgaggt aaacttacat tatacttgct tcttatatat
     4681 ctttttgcca aaataccaat aggtgttcct gctctagcaa gtgaaactag tactacatca
     4741 tttccatttt ttttaattat tttttcacac ataactccta tagaaaatgc aagcttatct
     4801 ttattttctc ttagtttctc atgatataaa ttcagataat cttcactaac ttcatactct
     4861 attggtatca tttctgaata atgtctacca ttttgtattt ttaattctct ttctttattt
     4921 ccttcttctt caattaaatt tgatatatct tttaataaaa aaataacatc atctttagaa
     4981 tacgtgctaa aatctctcat atgactcaac tcctatctta aaatcgtgat atattttatc
     5041 ctgttaaaac actttagcta caacatacta tagttaattc ttttatactt gtttttttaa
     5101 ttatatcaat aaattctaga taagaatttt cacttttatt cagctctaca aataaataac
     5161 acttagaaaa attatgttta tctatattgt aaatataatt ttgaatatct atattataaa
     5221 aactctttag cataaattta cttttaatag gataatgctt cttatcaata ttaataattg
     5281 gacttcttgt tgttgaatga tagtatatat catattgatt ttctttttct tttgcaaaca
     5341 gcataggtat atacataaat tcttcagtac ctaaaaatag aatcttttct ttttcatttt
     5401 ttaaagattc tttactggcc atagttattt ttttactttc tctcttaacg atttctttta
     5461 atctaaattg ttctcttcta ttaattccaa atcttcctgt atattttaca tatttcttat
     5521 ttccaatata atcttctaaa tcaacataaa tatgatttac tactaaattt actttatcac
     5581 ttgaaatagt ctctaattta gtaatactat aattatcgtt gtaatttgaa ataatatctt
     5641 catctatata aaatttaaag tttcctttaa ataagtatac aaaatctatc ttacaattta
     5701 gctctctttc taaacgagta actttactta aattttcatc acttacccaa ttgagtattg
     5761 agcatatagt gtatttcttt ttaggataaa tgctttgaat tttcctaatt atattcatgc
     5821 atgtattggc tgtggtaatc tcatcatcaa ccaatattat ctcatctcca aatttaaagt
     5881 tgttaagcat accaaagtat aaattctggt ctgttgcatg ggaatgttcc tctaaaaatt
     5941 ccagattttc taactcgtca aactgctctc tagtagtatg aataaattca taatctccac
     6001 ataagtaatc aaaaaaacta tgcccaacag ctgtaccagt ttcagcaaaa gctataacag
     6061 taccatttga ttcttcctta tttttttcat tataaattgt agccattaat tttcccaaat
     6121 tatccatctc acttggctta catgctaaat gtttaccgag tttctttgag ataaacaaaa
     6181 aacgtctttt gctattattt cttactccta cgtctaaaaa atcatttagt tctaaattca
     6241 atggattatc aataatgtca atctcacaat taaaatttaa gcaattcttc atagtctact
     6301 cctttattta atacaccata tattttagat agaatcaatg tttttttagc ccataaaaaa
     6361 tgtggatttg tttcattcat tctctctcct aatacaccct tgctcacacc atacttactg
     6421 tctgtactat ccagtatatc caaagcatca gtaaaatctt catatgatac tgtagatagc
     6481 gcttgtacaa tttgaatttg cattggatga attacggtct ttccaattaa tccatgatat
     6541 ttatccaata atatttcttc tatatactta tttcttatat ccttatttgt catatcaaaa
     6601 aattctgata ctccaccact tataggtata tctaactctg gtctttgaag atatgtgatt
     6661 atatctaata taaatgaaga acaaatcaaa ttattatata tagagaagtt tttatctctc
     6721 cttataccat acatccctaa tatatcagtt atgcctactc taatgcttaa tattcttgac
     6781 ttatgtctta aaatagcatt gtacaaatta gtaaaactaa gttctttttt ttcattatat
     6841 ataaattctc ttgtctctat tattggaata acatacatat cttgaagatt catagaatat
     6901 aaagcttcta tacaattttc aatcaactcc gaatttgctt taggaattaa tattcctgtt
     6961 atagaatgac tatttttaat tataatctct ttaattctca acagttgctc cacatctttt
     7021 attcttacaa aaattagtgg tattccatct ttattagtta tactttcatt gctaatattt
     7081 tttaaaatta atctaagatt ttctatagcc tctaactctc catttactcc aacagcatct
     7141 tctaaacaaa tagctaatgg tctaactccc ttaacatccc ctataatact cttataaatc
     7201 atattatatt gagtagctgg aacgtataaa aaagcgccca gtgcatattt caacacatct
     7261 ttttcagtct tagcactaaa gtcttctggc tccttcaaaa aaattctctc caaatcatct
     7321 ttgcagatat aatcgaaata tttcaatgtc tagcctcctc atatacatag aaagggccaa
     7381 ctttatacaa attagccctt aatataatac tacttattat aaattatagt cgtatttaat
     7441 tcttccaatc tctttgtatt atccttaatt aaagctctat tgttttcttc tattctcata
     7501 gtttcatcta taccagactt gatagtattg tacatttttt ccaatgtttc tatttgaaca
     7561 gaacttgttc cagccattct agctatttgt gcactttgag tacttacatt ttgtgcattt
     7621 ctaagcaata attcattagt tttgtcatca agtgctttta gagattttgc ttgtaattct
     7681 tgctttttaa gtaatatagc ttgagataaa cattgtttaa atactggtaa agttacgaca
     7741 aatgcactat ttatttttct aattaaacca taattattat gttgcatacc tctaagcata
     7801 ggtattgttt ggatagctat attttctgct attcttaaat catagactct ttgatttaac
     7861 atatcataaa cttttaatag ttcttgataa ttgacaacat ccatttggtc tccactagtc
     7921 tctgattttt gtttaaagaa aggtagtatt tcattatcca tttcttctat tgccatttct
     7981 ccagcaacaa tatatttttg aagttcatta taaaactcaa agttttcatt taacatagca
     8041 cctatttgct tattagaaag tgctatatct ctttcgtatt gctcaagctc aatttgaatt
     8101 ttctcaactt ctcctcccaa tgtttcatac ttttgaaaca tcatttcaat ggcattatta
     8161 gcttttttga aaagtttttg aacgaaattt ggttccttag tttcttgaaa atcgtctaaa
     8221 tcaaacttat ccattatttt agttaaatga accagcattt tagaattttc ttcatttcta
     8281 tttaatttta ttgtatttaa aaggctatca gaaactcttg ctactccttc agatgctttc
     8341 ctaccaaact gtaaaatcgt atctggattt tctacttcta taagggatgt caatgcctca
     8401 acttccttag actttctcag ttcatttttt ttattatttg tatattccat tatatcgaag
     8461 ttttcttctt gaactgctgt aggcaaattc tcagtaacct ctacagagtt cttatctttt
     8521 gaaaaactat ttatatttat ccccatctaa aatcgtctcc tctttccaaa aattttttta
     8581 agtaaaggta tatcattttc aagcgaattt aatttaaggt catataccat tttatccaat
     8641 ctgtgtatat caatcaattg gttatttata acattctcta tattgttttc tcctcctgga
     8701 cataatatga taatatcaaa tccaatctta ctaagtaaaa gcataagaaa aactatttct
     8761 ttatcaaata caatactctt gtacatgtat ataatcaatt ttgggtttat aagtgagtaa
     8821 tcgaaattgt tcatcatatg aacaaatttc ttatccatgt gaagtactgt aaatagacct
     8881 tttatcttgt cttctccact taaaccattc aaaaacatac ttccttctaa caactcttcc
     8941 actttctcta aaatcataat ttgttgatta agtgctagtg ttgatatgga ataatcttta
     9001 ttatttaaaa cagagttttt atctattaca ccttttgaat ccaataaaaa ggataaacta
     9061 aaagcttctc tcgtaaaagc ttttgaaaat ctatctacat ctccattaaa ttcaataaat
     9121 gttgaacttt ccgaatctct taataaatct aaaaacttaa tatattcatt cttatcatta
     9181 tatattccat taatcttaga aagaaaattt ggagcttcta caatagcatc attaaactta
     9241 aatcctggtc ttaatttaag tggttgattc cagtatattg atatttcatc aacagttgaa
     9301 cttagaagta gattttttat ttttctatct tggagttgcc aaggttttat aaatcctgca
     9361 tcatttaaca attcctcact tatacgcttt gatgcttctg ccccaacagt aaaagctttt
     9421 tttacagaac ttttatctat tttttcccct aaaataaccc tttcttcaaa agaaattttt
     9481 tcttctatta tgttggcttc ttcaaactct atattgtatc tttctgattt taaaatatct
     9541 atattactct ttgaatttgg attaagatat aaaacatcaa aacctgccaa gtataatatt
     9601 aagagtaaat aaacttcatg tttttttata tctccataaa atattacttt tggagcttca
     9661 tttttattta tatctaaagc accgatataa atttttatcc atgccattat tttcactata
     9721 aaattttgtt taactctatc attagtaaat tctttttcct cttcttttaa cactctatcc
     9781 attgcttgct ttattttata atcaatctct tcaaatccca aaatatttat atctacatct
     9841 tccatgctag ttctatattt ttgaagtcca cttttaaaat aatttataat atcaaaatca
     9901 ctaggatttg ctataacatc agtaaataaa gcaatatctt ttttgctatc aatattttgt
     9961 tttgtcttaa atatctcgag attgtacaaa acttcatctt ctaaaccaat atacgaaata
    10021 aaataactta gatatccctt tgaaggagtg taatttcctc tatctgattg gctggagaat
    10081 attttttcaa atacctttga gtcacccata actatcctcc caatttaaat gtttattcag
    10141 aactgaccca aaattcatat tttgagtcag tctgttttct tctaaaaaat ttgttttaag
    10201 cagtcttttt tggttttacc agtcttatta tacattttac aacttcatta accacaatta
    10261 cacttgctgc tacacataaa acttttaccc atgtcataaa atccattgct acagagttaa
    10321 agaaatcttg gaatagctga gtaaatatta cttgtgcaat tcctgtcaac actattattt
    10381 gtagtgctaa tttgttatgt gtaaaattag ggaagatact atctgcatta aactctctac
    10441 agttaaatgc attaaacagt gcgctaaaag caaataacgc aaacattact gttcccttat
    10501 gagcttcatc aactcctaag aaattaaatg tagattgtaa cattagtatt gctgttatat
    10561 aaaatgcatt taatactatt gtatacacca ttgacttaga aattatattg gcttttctat
    10621 caacaggttt tctatttaac acatggtctc taactggctc taaacctaat gctagtgctg
    10681 gtggtccatc cataattatg tttacccata ataattgtat tgtagtaaat ggcatctctt
    10741 gtcccataac ttgagatatt atagccacta aaaatgcaat tacatttaca gttaattgga
    10801 attgaacgaa tctttggaag ttatcataaa ttcctctacc ccatttaata ccttttacaa
    10861 tggtactaaa actatcatca gttaaaatta tgtctgctgc gtttttagat acctctgtac
    10921 cagatatacc cattgctata ccaacatctg cttttgaaag tgctggagca tcatttatac
    10981 catcacctgt tactgcaaca acatccccgc tcttttgcag agctgaaact attctcattt
    11041 tactatctgg tttacttctt gccacgattg aaatactttg tatctcttgt tttaattctt
    11101 catcatctaa agtatctata tatgaagctt caaccactct catattattt tttaataggc
    11161 ctaactcttc acctatagct cttgccgtat ttatattatc tcctgtaagc atttttacag
    11221 aaacaccagc tttatttgct gtatttacag actctataac atcttttcta agtgggtctc
    11281 ttataccaac aaaaccacta aatactaaat tattttccat tgcacttaca ttcatagcat
    11341 cactttcagc agttactgct gcttcttctt cagcttgtga gatttcttta tatgcaaacc
    11401 ctaaagttct cattgatttt atttgcaatt tctttatttc ttctaaaatc tcatgtttta
    11461 tattttcagt taatggaact atgtcttttc ctctttgaat atgtgaacat aattcaagta
    11521 agacttctgg tgctcctttt gataatagta catgatgatt atcttgacct ataacagaag
    11581 tcattctctt tgaatctgaa gtaaaaggat tttgagctac tatattagaa ttttttctaa
    11641 cttgtctata atctacgttt ttgtaatata aaagcaatgc acattcagta gcacttccta
    11701 aatacttaat atcaccatca ttgtgctcta catcagctgt agagtttatc atacagtttt
    11761 cttcaaaata actatttatt tcctcttctg aactttcaat atatctgcca tcaacatatg
    11821 caacttctac agtcattcta ttttcagtta atgtaccagt tttatctgaa catattacag
    11881 aaacagaacc tatcgtttca caagcttctt ttttggtaac caatgcattt attttagcca
    11941 ttttttgcat tgttattgca agtgtcatat ttatcatagt tggaagacct tctggcactg
    12001 ctgcaactat caacgcaaca catactgtaa atgctgtttt aactgggtct attgattcta
    12061 aaaacggtaa tattccagac atatctacat ttaatacatt cttacttatc atttggaaaa
    12121 ccataaatat aaatagtaat cctgctactg cactagaaac tttggctatc aaaccaccta
    12181 aatttccaag ttttatctga agtggagttt gctcatctgt ttctgataag ttttgagcaa
    12241 ttttacccat ttcactatta tctccaattg aagttacaac tagagctcct ctaccataag
    12301 ctacaagtgt accaccaaac accatattta tctgttttgc aggtattggc tcctgtacta
    12361 ctactgctcc tttagattct atctcttcca tagaaactac tacatcacac tttttagata
    12421 cgtcatcaga ttctcctgtt aacatatctt ctctaatttt aaggtttata ctttctataa
    12481 gtctaccatc agcaggtacc atgtcaccag tttcaatata tactatatca cctggtacca
    12541 attcactctt agaaatttga tgtacttccc catctctcaa tacttttact tctatgtttt
    12601 cagtcaactt agaaagagcc tctgctgctt tcttagaacg accttctgta atcataccta
    12661 ttgctatacc tattgctata gcacctacaa tccctattgc atcatgagtt tccccaatta
    12721 ctgcacttac tactgctgca ataatcaaga taactatcat tggttcactt aaactttctt
    12781 ttaaatcatc ccaaaaacta ccctcttctt ttggagtaaa ttcattcaaa ccatattttt
    12841 ctcttctagt cttgacttct gattctgata aacctttttc aggattaaca gataagtctt
    12901 ttgtaacttc ttctttatct acattataat atttgatatc attattaaat tccatatgca
    12961 cactcaatcc tgtctaaatt ttgcattact tctttaagac ccgcatcttt tgttccttca
    13021 ccaattgctt taaattgcca acttccatct tttctatata tagctcctac tataagagta
    13081 gttttttcag aataattatc agatagattg tattttgcta tctctttctt attaccttgg
    13141 tcttcaactc ttatataggc attttctatc attccaaagt gttgttttct atctacacaa
    13201 ttgtatatat ttactacaaa caataattta tgaatatttg atggaacttt atccaaatta
    13261 actaggattt gttcatcatc acctgtaccc tcaccagtta gattgtctcc tgtatgcaaa
    13321 actgatttac aggcactttt tagatttcca aaataaatta aatcttttat tccacttaat
    13381 ttaccttctt gattaagcat aaatacagat gcatcacaat ctatatcagc ttgtcctcca
    13441 ccaaataacg atttaaaaaa acctcctcca gattgttgca cagggtccca tcctagacct
    13501 acaagtatgt tactcagtcc tgggttagat ttttttaaat ctattttatc accttttgat
    13561 aaattaatcg acatactaaa cctccacaaa attttatata atataaccta tacattattt
    13621 gtataggtta tttatattta atatattata ttgcttaaat taaacattta agccatagtt
    13681 tctacaaagt gcttctaagc caccttcaaa tcctgctcca actgcactaa acttccattc
    13741 tccattatac ttatatatct cacatactgt aattgcagtt tctatactaa attcttcacc
    13801 taagtcatat cttaatattt cttcatcatt ttcagaattt aatactctta tatatgagtt
    13861 gcttacttgt ccaaaatttt gtcttctttc agcagcctca tatattgtaa ctgttacagc
    13921 tatcttttgt atatgctttg gcattgtctc taaattcact aatatttcct catcatctcc
    13981 atcaccttca ccagttctat tatcacctaa gtgttcaact gctccagatg gatgtcttaa
    14041 gttattgtag aatataaaat cttcatcttt ttcaactctt tgagattctc caaccatgaa
    14101 aacacttaca tctaaatcat aatcatagcc actatcaaat gaatttgtat cccatcctag
    14161 tccaagtttt atatttttaa gacctggatt tcccttagtt aaatcgattt tttgtccttt
    14221 ttttaatact atagccatgg aaaatatccc cctttagttt attgttatag ttgaaattat
    14281 aagtttattc caaagtttcc acataatgca gcaagaccac cttcaaaacc agaacctagt
    14341 gcattaaact tccattctcc attgtgctta tatatttcag ctactactat agctgtttct
    14401 atactaaaat cttctcctaa ttcatacttt attaactctt catttgtgtc tttatctact
    14461 attcttatat aagaattact tacttgtcca aaattttgtc ttctttctgt cgcatcatga
    14521 attgtaacag taaaagaaat cttgcttatt tctccaggaa tcttagataa atcaacaact
    14581 atttgttcat catctccatc accttcgcca gttctgttat ctcctagatg ctcaactgct
    14641 ccagatgtat gttttaaatt gttgtaaaat acaaaatctc catcattagt aacacttcca
    14701 cttgctcctg ttaaaaatgc tgctgtatct aaatcaaagt caaatccacc atcatacttg
    14761 ttagtatccc atcctaaacc tactaaaata tttttaagac ctggatttcc tttagttaaa
    14821 tcaacctttt ggccttttga taatgttata cccataattt atcgctccct tgttaatttt
    14881 agctaaacaa atcttgctaa ttatttatct ttttctttaa tcatattata acatttttta
    14941 ttcatttgtg aagaaaactt gcttgatatt tttaaaatat aattaaaaaa atgtttatat
    15001 ttcttacttt ttacatagat tttacatact ttattaaatg tataaaagct atgtttaata
    15061 tgtaattata tatca
//
LOCUS       DAMJWP010000080        15064 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.084, whole genome shotgun sequence.
ACCESSION   DAMJWP010000080 DAMJWP010000000
VERSION     DAMJWP010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15064
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.084"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(270..413)
                     /gene="scfA"
                     /locus_tag="PC700_001992"
     CDS             complement(270..413)
                     /gene="scfA"
                     /locus_tag="PC700_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HDF2581303.1"
                     /translation="MENKHVKTLSQATLKQSAAKGGCGECQTSCQSACKTSCTVANQE
                     CER"
     gene            complement(479..841)
                     /locus_tag="PC700_001993"
     CDS             complement(479..841)
                     /locus_tag="PC700_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HDF2581304.1"
                     /translation="MGKSNYIFKGLGYAYIITLAVLLVYNLFLTFTDIGGDNITMVSS
                     FITTISAAIGGFYTSKHMKEKGLMYGLLVGLLYIVCIFLTVFLAQEKFVFEVGMIYKL
                     LLISAAGGIGGVLGVNFK"
     gene            complement(899..1192)
                     /gene="yajC"
                     /locus_tag="PC700_001994"
     CDS             complement(899..1192)
                     /gene="yajC"
                     /locus_tag="PC700_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDF2581305.1"
                     /translation="MPQQQIIMSIGLWVVVIAIFYFLMIRPQKKKDKQLKEMRSSLSV
                     GDKVITIGGIVANVAKVEDDVVILELGPNRTKVPFEKWAIGTVKSKKEEIEED"
     gene            complement(1326..2447)
                     /gene="tgt"
                     /locus_tag="PC700_001995"
     CDS             complement(1326..2447)
                     /gene="tgt"
                     /locus_tag="PC700_001995"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDF2581306.1"
                     /translation="MYAVRYELIKTCKQSGARLGRLHTPHGIIETPIFMPVGTQATVK
                     SMTPEELKEIGSQIILSNTYHLYMRPGHELIKRAGGLHKFMNWDKPILTDSGGFQVFS
                     LGPLRKIKEEGVEFRSHLDGSKHFLTPEKAMEIQNALGSDIMMAFDECAPYPSDREYV
                     KNSLERTTRWLKRCKDAHNNTDKQALFGIIQGGMYKDLREQSAKEITSIDLPGYAIGG
                     LSVGEPKPLMYDVLEHTTPLMPQDKPRYLMGVGSPDDLVEGVIRGVDMFDCVLPTRIA
                     RNGTAMTSQGKVVVRNATYAEDFTPLDPECDCYACKNYSRAYIRHLIKANEILGARLI
                     TTHNLHFLLNLMKQIRQAIMEDRLLDFRNEFFAKYGYEI"
     gene            complement(2526..3011)
                     /locus_tag="PC700_001996"
     CDS             complement(2526..3011)
                     /locus_tag="PC700_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581307.1"
                     /translation="MHSLQKQKKNNPILKIAFIFERVLAVVVLIAVFLGTIDVLRLMW
                     GAYIVDFQNPVQYSQLNDFLAQILLLVIGVELVVMLSLHIPGAFIEALLYAIARKMLL
                     LPKNEGMIDVLIGVIAIAGLFAIKKFLVTKDKSALNIMSIHDEEAIKEQQNKEDEDKS
                     I"
     gene            complement(3023..4048)
                     /gene="queA"
                     /locus_tag="PC700_001997"
     CDS             complement(3023..4048)
                     /gene="queA"
                     /locus_tag="PC700_001997"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDF2581308.1"
                     /translation="MKTSDFKFDLPQELIAQVPIEDRASSRLMVLDKETGNIEHKVFR
                     DIIEYLNPGDCLVLNNTRVIPARLIGEKLETGGKIEFLLLKRTEEDTWQALVKPGKRA
                     KVGTKFSFGNGKLIGEVVDLSDEGSRIIKFLYDGIFEEILDELGNMPLPPYITARLDE
                     KERYQTVYSKHNGSAAAPTAGLHFTEELLNKIKEKGVDIAFVTLHVGLGTFRPVKVED
                     VLNHKMHSEYYMVSQEAADKINRAKENGNNVICVGTTSCRTIESACNEDGKMKETSGW
                     TEIFIYPGYKFKVLDKLITNFHLPESTLIMLVSAICGKDNVLNAYNEAVKERYRFFSF
                     GDAMIIK"
     gene            complement(4366..5385)
                     /gene="ruvB"
                     /locus_tag="PC700_001998"
     CDS             complement(4366..5385)
                     /gene="ruvB"
                     /locus_tag="PC700_001998"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDF2581309.1"
                     /translation="MQGFEDENRIITSTMKMEDIDIENSLRPKTLEDYLGQEKSKEQL
                     SIFIEAAKSRNEQLDHVLLYGPPGLGKTTLASIIANEMGVNLRITSGPAIERAGDLAA
                     ILTNLNENDVLFIDEIHRINRSVEEVLYPAMEDFCLDIIIGKGPSARSIRLDLPKFTL
                     IGATTRAGMLTNPLRDRFGVICKLDYYTVDELSKIVLRSSSILDAEIQSNGALELAKR
                     SRGTPRIANRLLKRVRDFAQVRADGKITDKVAKDALELLGVDSLGLDFVDEKLLMTII
                     EKFRGGPVGLDTLAASIGEDRNTIEDVYEPYLLQLGFINRGPRGRVAMPLAYEHLKIP
                     YPNEK"
     gene            complement(5492..6094)
                     /gene="ruvA"
                     /locus_tag="PC700_001999"
     CDS             complement(5492..6094)
                     /gene="ruvA"
                     /locus_tag="PC700_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDF2581310.1"
                     /translation="MYSYIKGTVEEIYIDSIVVENNGIGYKINVSSNTIMNLQVGEAT
                     KIYTKLIVREDDMSLCGFVSREELKMFELLTSVSKIGPKVALSILSFASSAQLGAYIL
                     SEDIGKLSKAPGVGKKTAERIVLELKDKVDKNNIEFEPTLLSQKPTLISQDESVDALV
                     ALGYTLSESKEAVQKCKKDGMNTEAIIKKALTYIMSKSLK"
     gene            complement(6087..6596)
                     /gene="ruvC"
                     /locus_tag="PC700_002000"
     CDS             complement(6087..6596)
                     /gene="ruvC"
                     /locus_tag="PC700_002000"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HDF2581311.1"
                     /translation="MIILGIDPGIAIVGYGIIEYKNSKFKVIDYGAVTTPAHMNISRR
                     LELVYKGIDTIVKNYNIDEVGMEELFFNKNVKTAITVAQARGVTMLACAHNGKPVYEY
                     TPLQVKQGVVGYGRADKAQVQQMVTSFLSLKKVPKPDDVADALAVAICHAHSNKLEKT
                     LKNIGGKYV"
     gene            complement(6908..7537)
                     /locus_tag="PC700_002001"
     CDS             complement(6908..7537)
                     /locus_tag="PC700_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581312.1"
                     /translation="MFEKKKKELFPWKISALILVGVFVLSSGVYIGTRLKNNGVQTSK
                     VVSASANMDEKSKEAFGLNKNCEVWLQSEGENETNYKRTLTMAGIVPESLLDKTKSEI
                     VSYFKKEYPTKKIKSMDKNEIILVESKYSDNKDVQAMNATIKGKYTIENDNGNIALYK
                     YDDSGSKTLVEKTKIRVDSLPKTVQDEIKKGVVMDTEEEAYSRLEDFAS"
     gene            7740..8063
                     /locus_tag="PC700_002002"
     CDS             7740..8063
                     /locus_tag="PC700_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HDF2581313.1"
                     /translation="MERINCNVINCSHNNSHICYADKIFINGEKAHNDRHTCCSSFLD
                     ESIYSNLSSNVNNRNCEELVCTVKNCKYNKDGSLCTLDNILVAPETDRVNLYIETCCS
                     SFECK"
     gene            8331..9185
                     /locus_tag="PC700_002003"
     CDS             8331..9185
                     /locus_tag="PC700_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581314.1"
                     /translation="MSTFYIQFLYFVIYSFIGWCCETTYCSVLQKQFVNRGFLNGPFC
                     PIYGFGALAIVATLTPFVHNIPLLFLFSIIITSIMEYCTSFILEKIFNMTWWDYSKHK
                     FNIHGRVCLENSLMFGVLSLIVMLIVHPIVVDFINSISKNVLFIFAISIEIYFVLDLV
                     ITVHTILQLNGKLKQINLIIKELKDKKEYYKLITQETIENKLESLVENRLDTLEDRFE
                     ILKEKENYRYAKNRIDELKNKLNTLLSNHKLLHRRIIKAFPNISSNKHTDILNKIKEN
                     IKSIKKDK"
     gene            complement(9371..10807)
                     /locus_tag="PC700_002004"
     CDS             complement(9371..10807)
                     /locus_tag="PC700_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HDF2581315.1"
                     /translation="MREVKPISIDILNTFKQVDEDRLNELLADELKHLDRKIVVLDDD
                     PTGVQTVHDISVYTDWDKDSMEQGFNEKNSMFFILTNSRGFTVAQTTKAHKEISKNIV
                     DVSKKVNKDFIIISRSDSTMRGHYPVETNLLKSEVERLSGKLFQGEIIMPFFKEGGRF
                     TIDNVHYVKEGEILVPAGMTEFAKDKSFGYKSSDIGEWCEEKTHGKYKSSDMIYISSK
                     ELRELNIEAITEKLKKAEGFNKIIVNATDYVDVKVFTIAFIRAVNSGKEFMFRSAAAI
                     TKVLGGVSDKEMLTKDELVSKGNTNGGIILVGSHVNKTTQQLEELKNCKYPIEFIEFN
                     QHLVLQENGLKNEVKRVIKVVEEKISSGKTVAVYTRRERFDLDTNDKDKQLMISVEIS
                     DAVTSIIGMLNVRPNFIIAKGGITSSDVGTKALRVKKATVMGQIKPGIPVWMTGKESK
                     FPNMPYIIFPGNVGEISTLRESVEILMG"
     gene            complement(10878..12260)
                     /locus_tag="PC700_002005"
     CDS             complement(10878..12260)
                     /locus_tag="PC700_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDF2581316.1"
                     /translation="MITGYGLLIAFVISIGILLVSIIKFKVNPFLALLITSIITGFMV
                     RMPINEISTTISTGFGNTLGSIGIVIGLGIIFGNILSESRATESIAKGLLAKTGEKNS
                     ALAVTTAGFLISIPVFMDAAFVIMMPIIKYVSRVTKKSLMVFVCALGVGTIVGHALVI
                     PTPGPLAVAANVNANVGSFILYSIIVAFPAVLIGGWIYGKRFEKYPAYAIDENDREKN
                     LEKGKDSIKIEDDDSSKVPGFGISMFSLLFPILLILISNVFSMFLEKGSTMSGVLAFI
                     GDKNIAILLGILVAIGFLKRYINKPMGDVVIEAADSAGLILLITGSGGAFGSVINASG
                     IGNFLVDTMSGLSISVVVLGFLLSALLRISQGSATVALVTTSSILGPTILATGMSPVL
                     VGLAICAGGVGFSLPNDSGFWVLSRFSGLSVKDTLNSWTIGGTIAGVVAFIMVLLLSV
                     INGIIPLPGL"
     gene            complement(12396..13301)
                     /gene="garR"
                     /locus_tag="PC700_002006"
     CDS             complement(12396..13301)
                     /gene="garR"
                     /locus_tag="PC700_002006"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HDF2581317.1"
                     /translation="MEGYKMKLGFIGLGIMGKPMAKNLLKDGCNLLVYDINKSAVDEL
                     ISCGAKYASVLEMGQECDIVFTILPNGTIVQDILFGMDGLAKTLKEGSIVVDMSSVTP
                     TESILCANKLKDMGLEFIDSPVSGGEPKAIDGTLAFMAGGKEEIYKKVEPFFNIMGSS
                     SILIGDNGSGSVTKLTNQVIVNLTIAAVSEAFVLAAKAGADPEKVYKAIRGGLAGSTI
                     LDAKIPMIMNRDFKPGGKISINLKDIKNVMQTAHNLDVPLPMTSQLLEIMQTLKVHGH
                     LEDDHSGIAQYFEKLAGVEIKKHNN"
     gene            13697..14362
                     /locus_tag="PC700_002007"
     CDS             13697..14362
                     /locus_tag="PC700_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2581318.1"
                     /translation="MATFKRLQEQAYDYLKELILSGEMIENEIYSETKLASEIGISRT
                     PIRDALQRLSQDGFIDIIPSKGFRIHQITANEIVEIFQIRSAIEGFCTFLITSQYKEA
                     RAVETISKLKHLLDKQKDILLGDKNLNSFAEYDTLFHTTIVSYAQNTEFDKMFNNYMY
                     RIKKLALDSLSHEGRLETTLKEHTDIFNNIANGCIEDVYKTTLIHMETPKYINLEDFC
                     NNK"
     gene            14470..15018
                     /locus_tag="PC700_002008"
     CDS             14470..15018
                     /locus_tag="PC700_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581319.1"
                     /translation="MIFLNENVVTNETIDKDYAIEEVRMACKHFGDLYFYFSKVLFEE
                     FGEDKTIEILRKVLFERSEERAIAMRERALENGDELIADNIISTTDVPFLGWVPEFKE
                     LHCPYGASWLLRFEENPWFKKFASLYCDVTDTTVAEVFTGDTSHKITKNILWGDKSCE
                     RIYFHDDKVSEGKYTYGNKNCK"
BASE COUNT         4968 a         2637 c         1736 g         5723 t
ORIGIN      
        1 taaaatcatc ataaattaga gtttaaaatt aaaaattatt ctaatacatg taattgataa
       61 aaaataaaat ataagagtag ctactttaaa ataaatgctc ttatatgatt agatattttt
      121 gcataattaa acgataagaa atcaaattta attttacata agtagaatat atatttttat
      181 ataataattg aaaataaaag aggatacctc ataatgataa aagaatcact ttgagatacc
      241 ctcttacaaa caactaaatc aataattttc tatctttcac actcttgatt tgcaactgta
      301 caagaagttt tacaagctga ttgacaagaa gtttgacact ctccacatcc accttttgca
      361 gcactttgct ttaaagtagc ttgagataaa gtttttacat gtttattttc cattttcaac
      421 gcctccattt ctaattgttt cctattgatt atatcataaa actactttaa tataaactct
      481 atttaaagtt gacacccaat acgcctccaa tgcctcctgc tgctgatatt agcaataact
      541 tataaatcat ccctacttca aatacaaact tttcttgtgc taaaaacaca gtcaaaaaaa
      601 tacatactat gtacaaaagt cctactaaaa gtccatacat aagtcctttt tctttcatgt
      661 gttttgaagt gtaaaaccct cctatagctg cagaaatagt agttataaat gatgagacca
      721 tagttatatt atctccacca atatctgtaa atgtcaaaaa taagttatac actaataaaa
      781 cagccaaagt tattatatat gcatatccta gacctttaaa aatataatta gattttccca
      841 taaaaaaacc tcctctcaaa ttaatatata ttcgagaaaa ggcttttaaa ttactaattt
      901 agtcttcttc tatttcttct tttttagatt taacagttcc tatagcccat ttttcaaatg
      961 gaactttagt tctatttggt cctaattcta atattacaac atcatcttca actttagcta
     1021 cattagctac tattccacct attgtaatga ccttgtctcc tacacttagg ctacttctca
     1081 tttcttttaa ttgtttgtct tttttctttt gaggtctaat cattaggaaa taaaagattg
     1141 cgattactac tacccataga cctatactca taattatttg ttgctgtggc attaattttt
     1201 cactcctttg taaattatac tcttttgtaa atcataataa atgtttatat aacaatttac
     1261 tttctctatc tttgagtact attgaatatt atatattaaa atgattataa ttaatagtgc
     1321 aaaacctata tttcatatcc atattttgca aaaaattcat ttctaaaatc aagtaatcta
     1381 tcttccatta tcgcttgtct tatttgtttc attaaattta ataaaaagtg aagattatga
     1441 gtagttatta atcttgcacc cagtatttca tttgctttta taaggtgtct tatataagct
     1501 cttgaatagt ttttacaagc ataacaatca cattcagggt caagtggagt aaagtcttca
     1561 gcataagttg catttctaac tactacttta ccttggctag tcattgcagt tccatttcta
     1621 gcgatacgag taggtaggac acaatcaaac atgtctactc ctcttataac accttccact
     1681 aagtcatctg gactaccaac tcccattagg tatcttggtt tatcctgtgg cataagtgga
     1741 gttgtatgct ctagcacgtc atacataagt ggttttggtt ctccaacact aagcccacca
     1801 atagcatatc ctggtaaatc aatactagtt atttcttttg ctgattgttc tcttaagtct
     1861 ttatacatcc caccttgtat tataccaaat aaagcttgtt tgtcggtatt attatgggca
     1921 tctttacatc tttttaacca tcttgttgtt ctttctaaag aattttttac atattctcta
     1981 tctgatgggt atggtgcaca ctcatcaaac gccatcatta tgtctgaacc caatgcattt
     2041 tgtatttcca tagctttttc tggagttaga aaatgttttg aaccatctaa gtgtgaccta
     2101 aattctacac cttcttcttt tatctttctt aaaggcccta agctaaatac ttggaaccct
     2161 ccactatctg taagtattgg tttatcccag ttcataaact tatgaagacc accagctctt
     2221 tttataagtt catgacctgg tctcatatat aaatggtatg tgttactaag tattatttgt
     2281 gaaccaatct cttttaattc ttctggtgtc atagatttta cagttgcttg agttccaact
     2341 ggcatgaata ttggtgtttc aataatccca tgtggagtgt gtaatcttcc taaccttgca
     2401 ccactctgct tacaagtctt tatcaattca tatcttactg cgtacataaa gttctcctta
     2461 accatattta tttctcatta tttcttaaga tatatttgta ataagctatt catataattt
     2521 attgtctaaa tagatttatc ttcgtcttct ttgttctgtt gctccttaat tgcttcttca
     2581 tcatgaatac tcattatatt aagagcactc ttgtcttttg ttactaaaaa cttttttata
     2641 gcaaatagtc ctgctatagc tataactcct attaaaacat ctatcatacc ttcattttta
     2701 ggcagtagca acatttttct agctattgcg taaagtaatg cctcaataaa tgctcctggt
     2761 atatgaagag atagcattac tacaagttca acacctatta ctaaaagtaa tatttgtgct
     2821 aaaaaatcat taagctgact gtattgtact ggattttgaa aatcaactat atatgctccc
     2881 cacataagtc ttagaacatc tatagttcct aaaaatactg ctattagaac tactactgct
     2941 aaaacacgtt caaatataaa agcaatcttt aatatagggt tattcttttt ttgtttttgt
     3001 aaactatgca ttcattacct ccctacttta taatcatagc atctccaaaa ctgaagaatc
     3061 tatatctttc ttttactgcc tcattatatg catttaaaac attatccttt ccacaaatag
     3121 cacttactaa cattattagt gtagattctg gtaaatgaaa attagtaata agtttatcta
     3181 aaactttaaa tttataacct ggatatataa atatttctgt ccatccacta gtttctttca
     3241 tctttccatc ctcattacaa gctgattcta tagttctaca actagtagta ccaacacata
     3301 ttacattgtt tccattttcc ttcgctctat ttattttgtc agctgcttct tggctaacca
     3361 tataatattc tgaatgcatc ttgtgattta aaacatcttc tacttttact ggtctaaaag
     3421 tgcctaaacc aacgtgtaga gttacaaaag ctatatctac acctttttct tttattttat
     3481 ttaacaattc ttctgtaaaa tgtaatcctg ctgtaggtgc tgctgctgaa ccattatgct
     3541 ttgaataaac agtctggtat ctttcttttt catctagtct agcagtgata tatggaggta
     3601 gtggcatatt tccaagttca tccaatattt cttcaaaaat tccatcataa aggaatttta
     3661 taattcttga accttcatct gataaatcta caacttctcc aattagcttt ccatttccaa
     3721 aagaaaattt agtccctact tttgctcttt ttcctggttt tactaaagcc tgccaagtat
     3781 cttcctctgt tctttttaat aataaaaact ctattttacc tccagtttca agcttctctc
     3841 ctattaatct agctggtata actctagtat tatttaatac taaacaatct cctggattta
     3901 aatattctat tatatctcta aatactttat gttcaatatt acctgtctct ttatctaata
     3961 ccatcaatct tgagctagct ctatcttcta taggaacttg agctataagt tcctgtggta
     4021 agtcaaattt aaaatcactt gttttcaatg ttaaacttcc tctcttaaaa tttctccagc
     4081 tacaataata tcaaaaaaac aacttatttt ccacaatatt accataatct atcaagaaat
     4141 tgctattttt aacagacacc atatccacaa tatatagata tttttccacg atatacatgt
     4201 cttatccaca atatatagat acaatacaca caatttttat cgtgtttatc cacagtatct
     4261 acagatataa ttaccatttt gtggatatat ttgaattttt agccatttta gaaatatttt
     4321 aatttaatga ctcttttatt ttataaatac tcgtataatc tatttttatt tttcattagg
     4381 atatggaatt tttaagtgtt catatgctaa tggcatggct actcttcctc taggacctct
     4441 atttataaat cctaattgaa gcaaatatgg ctcatataca tcttctattg tatttctatc
     4501 ttctccaata gaagctgcca atgtatctaa acccactggt cctcctctaa atttttctat
     4561 tatagtcata agaagcttct catctacaaa atcgagaccc aaactatcga ctcctaaaag
     4621 ttccagagca tcttttgcca ccttatcagt tatttttcca tcagctctga cttgtgcaaa
     4681 atctctaact ctttttagta atctatttgc tatccttgga gtccctcttg aacgttttgc
     4741 aagttctaat gctccattag actgtatctc tgcatctaag atgcttgaag acctaagtac
     4801 tatttttgaa agttcatcaa ctgtataata atccaattta caaataacac caaatctatc
     4861 tctaagtgga tttgtaagca taccagctct tgtagtagct ccaataagtg taaatttagg
     4921 aaggtcaagt cttatgcttc tagcagatgg ccctttccct attattatat ctaggcaaaa
     4981 atcctccatt gctggatata aaacttcttc aacacttcta ttgattctat gaatctcatc
     5041 tataaataaa acatcatttt catttaaatt cgtaagtatg gctgccaaat ctccagctct
     5101 ttctattgcg ggtcctgacg taattcttaa atttaccccc atctcatttg ctattatact
     5161 tgctagtgta gtttttccaa gacctggtgg tccatataaa agcacatggt ctaactgctc
     5221 atttctcgat ttagctgctt ctataaatat actaagttgt tctttggatt tttcttgacc
     5281 caaataatct tctaaagttt ttggtcttaa actgttttct atatctatat cttccatctt
     5341 cattgtagaa gttattatcc tattttcatc ttcaaaacct tgcatttata cccctcctct
     5401 cgtacttttg aaaatctact ttatatattt tcaaatttac ataaactcct taaatattgt
     5461 tttaaaatcc aaatattata aaatccaaat actatttcaa ggatttactc attatatatg
     5521 taagagcttt ctttataata gcttctgtat tcataccatc ttttttacat ttttgaactg
     5581 cctcttttga ttccgacaaa gtatagccta aagctacaag tgcatctaca gactcatctt
     5641 gtgatatcaa ggtaggtttt tgtgataata acgttggttc aaactctatg ttatttttgt
     5701 ctaccttatc cttaagctct aagacgattc tttctgctgt ctttttacca acaccaggag
     5761 ctttggataa ttttccaatg tcttcactta aaatatatgc tcctaactgt gctgatgatg
     5821 caaatgatag tattgaaagc gccaccttag gtcctatttt agatacagat gttagtaatt
     5881 caaacatctt aagttcttcc ctacttacaa aaccacaaag actcatatca tcttctctta
     5941 caattaattt agtatatatt tttgtggctt ctcccacttg taaattcatt attgtattgg
     6001 atgatacatt tattttatat cctatcccat tattttcaac aactatactg tctatataaa
     6061 tttcttctac tgtacctttt atataactat acatacttac cacctatatt ctttagagtt
     6121 ttttcaagtt tgtttgaatg agcatggcaa atagccacag ctaaagcatc tgcaacatca
     6181 tctggttttg gaactttttt tagacttaaa aatgaagtta ccatctgttg aacttgtgcc
     6241 ttatctgctc tcccatatcc aactacacct tgttttactt gaagtggagt gtattcatat
     6301 acaggcttcc cattatgagc acatgcaagc atagtaacac ctctagcttg tgcaactgtt
     6361 atagctgttt ttacattctt gttaaagaat aattcttcca ttccaacttc atctatattg
     6421 taattcttta ctattgtatc aattccttta tacacaagtt ccaatcttct cgatatattc
     6481 atatgggcag gtgttgtaac tgctccataa tcgattactt taaacttgct atttttgtat
     6541 tcaattatac catatccaac tatggctata cctgggtcaa tccccaatat tatcattttt
     6601 taaaattccc cctaaaaata aaagaaatac caaaatgtat ttctcataaa aacttatgtt
     6661 ctaaatcatt atatcataaa taatattaac aaatcataaa atgctctatg taaaatataa
     6721 aatcaaataa aaaagttata tcaaagcaag attttacctc aatataactt ttatatttat
     6781 tacaaataaa ttattgtatt tttataggaa ttaaatgtga ctacaatttt aatactaatc
     6841 tatttaatat taaattattt attattaact tacttagtat ttaacttgaa ataatattaa
     6901 tttactatta acttgcaaag tcttctaatc ttgaataggc ctcttcttca gtatccataa
     6961 ctacaccttt tttgatttca tcttgaactg ttttaggaag tgaatctact cttatttttg
     7021 ttttttctac taatgtttta cttccactat catcatattt atatagtgct atatttccat
     7081 tatcattttc aatagtatat ttacctttta tagtagcgtt cattgcttgt acatctttat
     7141 tatcactata tttagattct accaatatga tttcattttt atccatactc tttatttttt
     7201 tagttggata ttctttttta aaatatgata ctatttctga ttttgtctta tctaataatg
     7261 actctggaac tatcccagcc attgtaagtg ttctcttata gttagtttca ttttcgcctt
     7321 cactttgaag ccaaacttca caatttttat ttaatccaaa agcttctttt gacttttcgt
     7381 ccatattcgc actagcactt acaactttac tagtttgaac tccattattt tttaatcttg
     7441 tccctatata aactccgcta cttaatacaa atactccaac cagtattaag gcagatattt
     7501 tccaaggaaa taattctttt ttcttctttt caaacattga ttacgcctcc tttttctatt
     7561 attttctcca agctgtaagt ttttatacaa tttaggtaaa ttttagaaga aaatatattt
     7621 atatttcaaa ttactctaag aatagatatt ttctaaaagg tagttttctc ttaatggtta
     7681 tattcttgta attatatgga cataatttat aataaataat cgtttaggag gtaataactt
     7741 tggaaagaat aaactgtaat gtaattaact gttcacataa caacagccat atatgttatg
     7801 cagataaaat ttttataaat ggtgaaaaag ctcacaatga cagacatact tgttgtagtt
     7861 catttttaga tgaatctatt tatagtaatt tatctagcaa cgttaataat agaaattgtg
     7921 aggaattagt ttgtactgta aaaaattgta aatacaacaa agatggtagc ttatgtacac
     7981 ttgataatat attggttgct cctgaaactg atagagtaaa cctttatatt gaaacatgtt
     8041 gttctagttt cgaatgtaaa tagtcatagt ataaatataa gggtgtcaaa tgtaattaaa
     8101 ctaacatagt gtagagtatc tacaactatt agtttgatta tacttgacac ccttatttat
     8161 tttaaataaa atttcattat tataaccatg tttataatgt tctaactgta attaaatttg
     8221 ataaatttct ataaattggt ataatcaata tgtaaattga atttagcaaa ttaatcataa
     8281 aaataaaata tcgagtatat agtgttatac taactagaaa tgaggtgctt atgagtacat
     8341 tttatattca atttctttat tttgtaattt atagctttat aggatggtgt tgtgaaacta
     8401 catattgttc agttttacaa aaacaatttg taaatagagg ttttttaaat ggtccatttt
     8461 gccctatata tggatttgga gcattagcaa tagttgctac cttgactcct tttgtacaca
     8521 atattccttt gttatttttg tttagtataa ttataactag cataatggag tattgtacga
     8581 gctttatttt agaaaaaata tttaatatga cttggtggga ttattctaaa cataaattta
     8641 atattcatgg aagagtttgt ctggaaaact cacttatgtt tggtgtatta tcattgattg
     8701 taatgcttat agtacatcct attgtggttg actttattaa ctctatctct aaaaatgtgc
     8761 tatttatatt tgctatatct atagaaattt attttgtttt agatttagtt atcacagtcc
     8821 atacaatttt acaattaaat ggtaagttaa aacaaatcaa tcttataatt aaggaattaa
     8881 aagataagaa agaatactac aaacttatta cacaagaaac tatagaaaac aaacttgaat
     8941 ctttagttga aaatagactg gatactttgg aagatagatt tgaaatactt aaggaaaaag
     9001 aaaactatcg ctatgctaaa aatcgtattg atgagctaaa aaataaactt aatactttgc
     9061 tgtctaacca caaattactt catagacgta taatcaaagc attccccaac ataagctcta
     9121 ataagcatac agatatttta aataaaatca aagaaaatat taaatcaatt aaaaaagata
     9181 aataataaaa taaacacata agttaaataa taaaaatact aatacataag attaaggttt
     9241 agaatatttt ttgtaaaata ctctaaaccc taattaatat actattattt ttaatcttaa
     9301 tttataattt tagtttattt tacttttaac acagtaaatt taagcgttta aacttcaata
     9361 cactaatata ctaccccatc aatatctcaa ctgattctct aagtgtgcta atctctccca
     9421 catttccagg gaaaattata tatggcatat taggaaattt gctttctttg cctgtcatcc
     9481 atactggtat accaggtttt atctgtccca ttactgtagc tttttttact cttagtgctt
     9541 tagtaccaac atcactagaa gtaattccac ccttagctat aataaaattt ggtcttacat
     9601 taagcatacc aattatactg gtaactgcat ctgatatttc tacagaaatc ataagctgtt
     9661 tatctttatc attcgtatcc aagtcaaaac gctctcgtct agtataaaca gcaacagttt
     9721 taccactgct tattttttcc tctacaactt ttataactct ttttacctca ttttttagac
     9781 cattttcctg aagaactaaa tgttgattaa attctataaa ttctattgga tatttacaat
     9841 ttttaagttc ttctaactgt tgggtagtct tattcacatg tgaaccaaca agtataattc
     9901 ctccattggt attacctttt gatactaact catccttagt aagcatttcc ttatcactca
     9961 ctccgccaag tacttttgtt atggcagcag cgcttctaaa cataaattcc ttaccactat
    10021 ttactgctcg tataaacgca attgtaaata cctttacatc tacataatcg gttgcattta
    10081 caataatttt attgaatcct tcagcttttt taagtttttc tgttatagct tcaatattta
    10141 gctctctaag ttctttagaa gaaatatata tcatatcact agatttgtac tttccatgtg
    10201 tcttttcttc acaccactca cctatatcag atgatttata tccaaaagat ttatcctttg
    10261 caaattcagt cattcctgct ggtacaagta tttctccttc tttaacataa tgtacattat
    10321 ctatagtaaa cctaccacct tctttaaaaa atggcattat tatttcaccc tgaaataact
    10381 tccccgataa tctctctact tcgcttttta ataaattagt ttcaacagga taatgtcctc
    10441 tcatagtaga atcactacga cttatgatta taaaatcctt attaactttc tttgatacat
    10501 ccacaatatt ctttgaaatt tctttatgtg cttttgtagt ttgagcaaca gtaaatcccc
    10561 ttgaattagt aagtataaaa aacatactat ttttctcatt aaaaccttgt tccatactat
    10621 ccttatccca atcagtataa acagaaatat catgaactgt ttgtacacct gttgggtcat
    10681 catctaatac aacaattttt ctatctaaat gcttaagctc atctgcaaga agttcattta
    10741 atctgtcctc atctacttgt ttaaaagtat taagtatatc tatgctaatt ggttttactt
    10801 ctctcattta aaacgcccct ttatgtaatt tgtatagtct aaatgtaatt tatataatat
    10861 aaacaatagt ttgttattta tagacctgga agtggtataa ttccatttat aacactaagc
    10921 aataaaacca ttataaatgc tacaactcca gcaatggttc ctccaattgt ccaagagttt
    10981 aaagtatctt taactgataa tccactaaat cttgaaagta cccagaatcc agaatcattt
    11041 ggaagggaga aaccaactcc tccagcacat atagcaagcc ctactaaaac tggtgacatt
    11101 ccagttgcca aaattgtagg tcctaaaatt gatgatgttg ttactaaggc tacagtagca
    11161 gaaccttgag aaattctaag caaagcactt aataagaatc caagcactac aactgatata
    11221 ctaagaccag acatagtatc aactaagaag ttacctatac cacttgcatt tataacactg
    11281 ccaaacgcac cacctgaacc tgttatcaat aatattagcc ctgctgaatc agcagcttca
    11341 ataactacat ctcccatagg tttatttatg tatctcttta aaaatcctat tgccaccaat
    11401 attccaagaa gtattgctat gtttttatct cctataaatg caagtactcc actcattgta
    11461 gaaccttttt ctaagaacat actaaataca tttgatataa gtataagaag tattgggaac
    11521 aataaagaaa acatagaaat tccaaaacct ggtactttag aagaatcatc atcttcaatt
    11581 tttatgctat cttttccctt ttctaaattt ttttctctat cattctcatc aatagcataa
    11641 gctggatatt tttcaaaacg ttttccataa atccatccac ctataagaac tgctggaaaa
    11701 gcaacaatta tagaataaag tataaaacta cctacatttg catttacatt tgctgcaact
    11761 gctaaaggtc ctggtgtagg tattacaagt gcatgaccta ctatagtccc aactcctaag
    11821 gcacatacaa aaaccattag agatttctta gttactctcg aaacatactt aattataggc
    11881 atcattataa caaaagctgc atccataaaa actggaattg aaattaaaaa acctgctgta
    11941 gttactgcta aagcactgtt tttttctcca gtcttagcta acaacccctt tgcaatagat
    12001 tctgttgctc ttgattctga aagaatattt ccaaatatta ttccaagtcc aattacaata
    12061 cctatactac ctaatgtatt tccaaatcca gtcgatattg tggttgatat ttcatttata
    12121 ggcattctaa ccataaagcc tgtaattata gatgttatca gtaatgccaa aaacggattt
    12181 actttaaatt taataatcga tacaagtaaa attcctatag atataacaaa tgctatcaaa
    12241 agtccatatc ctgtaatcat aatctttacc cctttatcat tctactttat tatttatcaa
    12301 ttttattttt cttttactat gataacttaa cctcgctgta tttatataat ctaatcctaa
    12361 taatatgtat ttaattatat tgatatattt aaatattaat tattgtgttt ttttatttca
    12421 acacctgcca acttttcaaa atactgtgct attccactat ggtcatcttc caaatgtcca
    12481 tgtactttta atgtctgcat aatttctaat aattgacttg tcataggtag cggtacatct
    12541 aaattgtgtg ctgtttgcat tacattctta atatctttca aattaataga tatttttcca
    12601 cctggtttaa aatcacgatt cataatcatt ggtatcttcg catccaatat tgtactacca
    12661 gctaggcctc ctcttattgc cttatatact ttttctgggt ctgcacctgc ttttgctgca
    12721 agtacaaatg cctcagaaac tgctgctatc gtcaaattaa ctattacttg gtttgtaagt
    12781 tttgtaacac ttccactacc attatctcca attaaaatag atgagcttcc cattatatta
    12841 aaaaatggct ctactttctt ataaatttct tccttaccac cagccataaa agcaagtgta
    12901 ccatcaatcg cttttggctc ccctccactt actggtgagt ctataaattc taagcccata
    12961 tcttttaatt tatttgcaca caatattgat tctgtaggag taactgaact catatcgact
    13021 actatacttc cttcttttaa agtttttgcc aatccatcca tcccaaataa tatatcctga
    13081 acaatagttc catttggaag gattgtaaat acaatatcac attcttgacc catttctaaa
    13141 acacttgcgt attttgctcc acatgatata agttcatcca cagcagattt attaatgtca
    13201 tacaccaaaa gattacatcc atcttttaat aaattttttg ccattggctt tcccattatt
    13261 cctaatccaa taaatcccaa tttcatttta taaccctcca tatcttcaca tttttgaaaa
    13321 cgttttttat gttttagagt atactacata ccagtatacc ggtcaatata aaatataaaa
    13381 gtatttattt taacaataaa tagtaatttt taaagtcatt tattatattt agtcatccta
    13441 tataagtttt aatacatact attgttttta aatataatat gctactttta cagttttgta
    13501 tttttactaa tttcaattag ttttaattaa tctgccttta atgttaatca tacttcacac
    13561 ttcaataatt ttttatattt tttaatctta tctgcaattt aaattgcaaa agattgacct
    13621 tattttcact atgttgtatg cttaaaatta tataagtttt tgtattttta tattgtttag
    13681 gggggtatta ttaagtatgg ctacatttaa aagattacaa gaacaagcat atgactactt
    13741 aaaagaattg atactatctg gagaaatgat agaaaatgaa atatattctg aaacaaaact
    13801 tgctagtgaa ataggtatct ctagaacacc tataagagat gctcttcaaa gacttagtca
    13861 agatggattt atagatataa tcccaagcaa aggatttaga atccatcaaa ttacagcaaa
    13921 tgaaattgta gagatattcc aaatacgctc tgcaatagaa gggttttgta cttttttaat
    13981 tacaagtcaa tacaaagaag ctagggcagt agaaactata agtaaattaa aacatctttt
    14041 agataaacaa aaagatattt tactaggtga taaaaattta aactcatttg cagaatacga
    14101 tacattattt cacacaacta tagtttcata tgctcaaaat actgaatttg acaaaatgtt
    14161 taacaactat atgtacagaa taaaaaaatt agctctcgat tcattatcac atgaaggaag
    14221 gcttgaaaca acattaaaag aacacacaga tatttttaat aatattgcaa atggatgtat
    14281 tgaagatgtt tacaaaacta ctctcattca tatggaaact cctaaataca ttaatttaga
    14341 agatttttgt aacaataagt gattttagta ttatactttc atttattaaa aatatatcta
    14401 cctcaaatta atttcctaat ttataaaaaa tattctataa ttatattgta actaatacta
    14461 ttctaaggag tgattttttt gaacgaaaat gttgtaacta atgaaactat tgacaaagat
    14521 tatgctattg aagaagttcg tatggcatgc aaacattttg gtgacttata tttttatttt
    14581 tccaaagtgt tatttgaaga gtttggtgaa gataaaacta tcgaaatact aagaaaagtt
    14641 ttatttgaaa ggtctgaaga aagagctatt gctatgagag aaagagctct tgaaaatgga
    14701 gatgaattaa ttgcagacaa tattattagc acaactgatg ttccatttct aggatgggtt
    14761 cctgaattta aagagttaca ttgcccttat ggagcttcct ggcttttaag atttgaggaa
    14821 aacccttggt ttaaaaaatt cgcttctctt tactgtgatg ttacagatac tactgtagct
    14881 gaagtattta caggagatac gtcccataaa ataacaaaaa acattctatg gggagataaa
    14941 agttgtgaaa gaatatattt ccatgatgac aaggtatctg aaggtaaata tacttatgga
    15001 aataaaaact gtaaataaat aatttaaatt ttctatattc atatactata ttttgtactt
    15061 tttc
//
LOCUS       DAMJWP010000081        14721 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.085, whole genome shotgun sequence.
ACCESSION   DAMJWP010000081 DAMJWP010000000
VERSION     DAMJWP010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14721
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.085"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(94..2232)
                     /gene="vanT"
                     /locus_tag="PC700_002009"
     CDS             complement(94..2232)
                     /gene="vanT"
                     /locus_tag="PC700_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HDF2581320.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            complement(2250..3056)
                     /locus_tag="PC700_002010"
     CDS             complement(2250..3056)
                     /locus_tag="PC700_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF2581321.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            complement(3053..4153)
                     /gene="vanG"
                     /locus_tag="PC700_002011"
     CDS             complement(3053..4153)
                     /gene="vanG"
                     /locus_tag="PC700_002011"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HDF2581322.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            complement(4414..5496)
                     /locus_tag="PC700_002012"
     CDS             complement(4414..5496)
                     /locus_tag="PC700_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2581323.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIVGNL"
     gene            complement(5546..6247)
                     /gene="vanR"
                     /locus_tag="PC700_002013"
     CDS             complement(5546..6247)
                     /gene="vanR"
                     /locus_tag="PC700_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanR-ABDEGLN family response regulator
                     transcription factor"
                     /protein_id="HDF2581324.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KDLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            complement(6681..9212)
                     /locus_tag="PC700_002014"
     CDS             complement(6681..9212)
                     /locus_tag="PC700_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF2581325.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVKEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLHSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            complement(9320..9937)
                     /locus_tag="PC700_002015"
     CDS             complement(9320..9937)
                     /locus_tag="PC700_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDF2581326.1"
                     /translation="MCKKTYLLVVSILIAGLLTACSGSPTKDSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIITPQQAMEIALKEQNG
                     MVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            complement(10299..11183)
                     /locus_tag="PC700_002016"
     CDS             complement(10299..11183)
                     /locus_tag="PC700_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HDF2581327.1"
                     /translation="MDTTKIDDISKLDLNNLDINKMDMGGIMESLLEWATTSGVKLII
                     GILILSIGFKIIKKFVNHVMLVLSKRDIDLTLRRFLKSLLLSVLKVAVIIIVLEYWGM
                     SLSSFAAVIASAGVAIGLALQGSLSNFAGGFIILLIRPFKVGDYVEAAGHGGTVEQIG
                     LFYTQLVTPDNKQILIPNGSVSNDSLINYSAKNTRRVDLTFSVGYEDDILHVRRVLKD
                     IVNNHKLIINEPEPFIGVVEHGDNAVKFATRVWCKTEDYWTIYLDLLEEVKVRFDEEG
                     ITIPYPKMDLTVKELNKI"
     gene            complement(11374..12624)
                     /locus_tag="PC700_002017"
     CDS             complement(11374..12624)
                     /locus_tag="PC700_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2581328.1"
                     /translation="MKSYTKAKWSVWGIITFSFVLVLFLRMSTAVVSDNLANELGFNS
                     IQISNIASFCLYAYAFMQIPAGILIDKYGARKISSLGIIMASLGSILFGLIQSIELAY
                     ISRVIVGAGTSVILLCILKIQGRWFNKSEFASATAKFSFVGNLGGVLATFPLVFLSEL
                     VGWRNSFLLIGIIGVVIGCFIYIIVRDTPKEYGFNVDTEPYEKSEKVNIVDGIKSVIK
                     NKSTWYNSMIMFSFVGLTSAFISLWGVRYIMDVYGVSKSFSAFIVSFFTYGFIFGSII
                     MDFVFAKIRSSKFNIIKFGAMIDLFIWIVIVVVYQVKPPIIVLPISFFIMGCIVMSHL
                     QVFNDAKYKNKEIYSGLATSVINTFEFIGSGIINLIIAISLQVNSYNIVDGYKKGFVV
                     FIVLSIITIVSSHIGVKNDDCKAI"
     gene            complement(12846..13610)
                     /locus_tag="PC700_002018"
     CDS             complement(12846..13610)
                     /locus_tag="PC700_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2581329.1"
                     /translation="MLGKLLKYELKASGRIFIPLYIAILIVAVFNGIFMNTNILQVQG
                     IGILVLTSLFMALGVLTIVVTIQRFRKNLLGDEGYLMFTLPVSTSSLILSKCITALIY
                     AVLSFIVAVFTFGVLMLFGTSGILLPEILDLFNTSFKWISENFLDILLLVVVMFISYS
                     SFILLLYTSISMGQLPKFNKHRNIVAFASFIAINIVISIVGDAVGSILPNEDTNMVYH
                     LYQSPSFMLAILGSLVVAIALFFATKFILDKKLNLE"
     gene            complement(13604..14329)
                     /locus_tag="PC700_002019"
     CDS             complement(13604..14329)
                     /locus_tag="PC700_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2581330.1"
                     /translation="MDKNISNVSQDGIVEFKGVNKSYGTKNVLKNIDLNIPKGKIVGL
                     LGPNGSGKSTMIKLMNGLLQPDNGEIMINGMKPSIDTKKIVSYLPERTYLNDWMKVSD
                     LLKFFYDFYSDFDVRRANEMIKSLDIDVNEKLKTMSKGTKEKVQLILVMSRNASIYIL
                     DEPIGGVDPAARSYILKTILKNYSEDSTLLIATHLISEIENICDEIIFISKGEIVLQG
                     DVEAIREEKGKSIDALFREEFKC"
     gene            complement(14331..14699)
                     /locus_tag="PC700_002020"
     CDS             complement(14331..14699)
                     /locus_tag="PC700_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2581331.1"
                     /translation="MEWELDNNKPIYIQLVEHLKLKIISGEIKIGSKLETVRALAEDA
                     EVNPNTMQKALTELERQGLVYSQRTKGRFVTDDKEKIKAMKEEIANVEINTLKVTLEK
                     LGYDRDEMLKLITENLKGEL"
BASE COUNT         5020 a         2661 c         1490 g         5550 t
ORIGIN      
        1 ataaaaaata tatattagac ataatattac taaaaatgtc tagtcagagt aaaataaatt
       61 acacagtaca acaaatatta ctatttaatt gagttacttc tttataacat ttaaccttct
      121 tcctactctg ctcaataatt cattcgatat actacatgag ttatttgcaa cttcagaagc
      181 agataattca gataaattat ctcttcctat tattattgcc gtattaccaa cttctacatt
      241 aggtatatct gtaatatcaa cagcaagttg gtccatgcaa attcgaccca ctattgctgc
      301 tctacaacca tttattatta cgtaactttt tccacaggat aaatttctag gtataccatc
      361 ggcatagcca atagataata tagcaattac gctatctctg cttgcaataa agactcttcc
      421 atatccaaaa ctatcatctt tttgtatttt tctaataaga attacttgtg attttaagga
      481 taaaacaggt cgtaaatcaa gttgcaattt agtcgtatca tttggagaac ttaatatccc
      541 gtataatgca attccaattc ttacataatt acacttaaga ttgggataat taaaaaaacc
      601 ataactactt tgaatatgta ctttaggaat ttttatacct ttttttaaca gaaaatcaat
      661 taacttataa aaacattcaa tttgtttatt tgtaaagtct acatcttcat ctttcagact
      721 atctgaaaca cacaagtgtg taaatatgcc acatatatct aagtatttta atttaaatgc
      781 ctttaaaatt gacctcacat cattaacatc aaaacccagt ctgtgcatac ctgtatcaat
      841 tttaatgtgt gattttatgt tgtatttctg tttgtttaaa gagatagcat gattaaggtc
      901 aataacactc tgtgtaagat tgtatttatg aagttccttt gcccttattg ggttagtata
      961 tccaagaatc aatatttctc ctagtattcc atatcgacgt aaatcaatcc cctcatctat
     1021 tgttgctact gcaaatgctt ttacacctat ttggtttgca caagtcgcta cttcaaaagc
     1081 actatgacca taggcattag cttttacaac tgccatcaat tcacaatctt ctggcatagc
     1141 tttttgtatc atctttacat tatgttccaa attatttaaa tcaatttcaa tccaagctct
     1201 attggtattt accttatgtt ctcctttcaa gtcttttttt agaggtttaa gaatcaatat
     1261 aataattata gaagctataa aagatgctat acttaccact atataatgaa taaggctatt
     1321 gtcaaccaag acagtttgta ttccgaatat tttagaaaac atacgaataa caacaatcat
     1381 cataggatgt ataatataaa taattaatga tgatgttcgc aataacctag ctcgagaacc
     1441 ttttaaaaat gttaaggctg taaataagaa atacatacat ggcaataaca taatatacat
     1501 gctatcatga cgctgtaccc ctagtttatg taaaaacatt ccctcacata acataagtaa
     1561 gaatgatata agaaatccaa tcaagctatt tttagttgac atataattag atttattaga
     1621 tattattcca ccaagtatga aaaatacagg tgcaaaaaat ataccatttc gtgtataatc
     1681 tgatacttca aaaatatacc catataaatt ctttaatagt ggtatttttt ctaaaaaacc
     1741 ataataacta tcaccaaaca taccaattac ataaataaca atagtgatta ctaatgagta
     1801 ctttaaacct agttttttta caagaaagta tgcaatactt gcaccaataa ttgatgcagg
     1861 tagataccat aaatgatata aagtcccatc aaatacaatg tccttaataa tattgggcac
     1921 taaattattc atggaaaaat atccagtgta aatatttaag ggaatataaa ttacaatagc
     1981 tatcccgtaa ataaataata cattttttag aaaagatttc agtttattac aattataatt
     2041 atatgttgaa attagaaaaa agcctgatag cataaaaaag aatggcacag caatacgagc
     2101 aattatacta gttaacacaa agtcagccat tttactatat gataataatg gagatgtatg
     2161 aattgcaatt ataagtaatg cagctataat cctaaaataa tcaagccctg cataagattt
     2221 attcctaatc attcttgttc ctccatttct tagtcctttc tatttctcca aaatgtcaca
     2281 ataaaaccat ccttattgtt acccgatatt tgatatacac attgttcagg tatagagatt
     2341 ctcacttctt ctttagtatg aataggcaca taaaagattt ctataatttt ttctttatct
     2401 tctattttta aacaatttgt atacgaaaat ctcttaatat attctatata ttcttcaagg
     2461 gcgagattca ttttattgat aatctcagaa tgtggatatc cgacataacg aaaatgccat
     2521 ggttcttgag atattcctgt tatttcttct tttttacact gataacgctc tataaaacca
     2581 taatgtagtg ctgccttacg gaactcatta caaataccat tataaggaaa ttcagggcag
     2641 ataaaatcta taacctcttt cttcaaccct aaatcaatag caagccctgt ctgatgttca
     2701 ctgtgatttg gaagtgctac aaacttttta gtaaagtctt ttccattgtc acgcagagag
     2761 cttgaatata tatcttgttg ttcctctgca gtacgatatc cacttacagg tactatttca
     2821 tttattccac atatactttt taaaatattt tgtaaaatat ttgtagcttc acgtttcata
     2881 agaatacttg gaaaccttgt atctacagga attaaatcta tatcttcaga tgttttaact
     2941 ggtaaaaaag catttactag aattagattc ccatcataaa ctttatcttt agtcaataag
     3001 atagtatttg tcattttatc ttctttattt ttatctaata aaatattatt catcatttta
     3061 tgtacacctc aattatctta tccaatattt cttcaaaaga cagtccaata cctttcatca
     3121 tattcggata acgactatga gaagtaaagc caggaattgt attgacttca ttaaaaataa
     3181 tttcaccaga tggagttaaa aacatatcta ctctagcaaa tccagaacat ccaagagttc
     3241 tataaataat ttttgctgtc tcttgtattc ttttctcaca atccaaatca attctagctg
     3301 gcatatgaat ttttgaactt ttcaatgtat atttttctgt ataatcaaaa aatccttttg
     3361 acaattctat ttcatctact cttccaacaa gtaagtcatc gtttcctaaa actgcacacc
     3421 ctacctcaaa accatcaata ttttcctcaa ttattacttc attatcatgt tcaaaagcca
     3481 agtctactgc tacttgtaac tcttcttttt tataaatttt actaatacca aatgatgaac
     3541 ctgcacgcac aggttttata aataatggat aagttaagtc ttttgacagt tcagccacat
     3601 taaatttatc tgagtatttt agtaatattg attttggtac tttaatacct tctgaatgaa
     3661 caagtttatg tgatttgtct ttgtccatac ataatgctga agaaagagta ccacatccaa
     3721 taacctgtat acctgccaat tcaaataacc cctgcactgt tccatcttct ccattttttc
     3781 catgcaatac aggaaataca aaatcaagtt tagtgtattt aatatcattt tcacatgatt
     3841 caatagcact atttggaata tgattctgag aaacagcatc aggaattaaa aattcaataa
     3901 taccattttt gacacggttc tgcgaaacaa taactggaat tatatcagta tccctttcaa
     3961 accaagtgtt attctggata ttattataat ctccaatata atgataccaa ttaccctccc
     4021 ttgtaattcc aattggtata atatcatatt tttccgtatt caaattctca agtactgcaa
     4081 aagctgattg taatgataca tcatattctg ttgagtttcc accaaatata actgcaattt
     4141 ttttattatt catctttgat tctcctttgt tgatttattt atttttatac aatctacaga
     4201 aacatgttaa aatacttttt ctatttcaaa tccaatcaac cttcacttac aattgcatct
     4261 gttccacata atatattttc acatttttct attgatataa ttactgtatc aaaataaaag
     4321 cactcttgtt ttgatacagt aattatatca ataacaagag tgctctttat ttatttatcc
     4381 taaagaattt cctatttttt tatacttaaa ttcttacaaa tttcctacaa tcggaataat
     4441 aaccttaaag gtaattatat tgttctcgct atgagctgtt attgtcccgt tatgaagctc
     4501 cactatttct ttagctatag caagtcccaa cccagctcta ccactgtttg aattacgaga
     4561 tgtatccaac ctgtaaaatt gttcaaaaat acgctcaagt ttttcctctg gaatcgtatt
     4621 gctatgattt ataaatttaa gctttatata atcatcctcc tgtacagctg ttatttcaat
     4681 agtagtatct tcaaaactat aataaacagc atttcgtaat aagttatcaa atacacgttg
     4741 cattttatta acatcacacc ttatcatcat atttggtgta acattcagtg tatattttaa
     4801 attcttactc atcaaagtag gtttaaactc atatgttagt tgttcaagca tacgagttaa
     4861 gcttactgta ctgtattcaa gtgatatatt tgataaatta aaccttgtaa tttcaaagaa
     4921 ttcattaatt aaatcttcaa gacgctctgc cttatccaac gaaatagata aatatttttt
     4981 tcgcagttct tcagaaatct gactttcatc tcgaagtagt gtcagatacc ctataactga
     5041 tgtaagtggt gtttttaaat catgtgcaag ataaacaata agttcattct tacgttgttc
     5101 tgccatttgt gtctcaaatt tacgttgttc aagtatatgt ttaattgagt ttattttctt
     5161 ttctattgcc aataactctg gagataaagc cacttctcca acatcttctt tgataagaga
     5221 gtcaatacct tgatttattt cactaaaata ttttgtaaac caatttaaat aaacacggaa
     5281 tattataaaa aacacaatca ccatagctag tatcatgaag agctccatgc tattacggaa
     5341 aatctgctga tataaattca atgcagcttt atattccata cgaaaagcat tttcaaagaa
     5401 tgatactatc cagatagaaa acttaccata caatataaaa gaatataaaa tccatatgaa
     5461 aataatggct ataaatgcca ttaaagctgt tctcataaaa attttatttc tcacttgagt
     5521 aaagtcagga ttgactttaa tatttttact tttcaattgt ataacctact ccccatacag
     5581 tttttataaa tttcgggttt ttagaaggtt catgaatttt ttcacgtaat cgccctatat
     5641 gagccataac tgtgttatta ttatcaagat atttttctcc ccatacagct tcaaacaatt
     5701 cttcagaagg aaccactttt ccttgatgtt cacataaata ccaaagaata gaaaattcaa
     5761 tcggtgttaa tgctacttct ttcccaaata aactacattt atgtgtttct ttattgataa
     5821 tcaatccttt aatatcatac tcattaacaa ttatcgactg ttgttcataa gaattattat
     5881 atcgcatata tcttcttaat tgtgtcttta ctcttgcaac aacttccaaa gggttaaatg
     5941 gctttgttat ataatcatca gcacctatgg ttaaacccat tatcttgtct atatcttcaa
     6001 ttttagctgt taacataatg ataggataat agtatttttc tcgaattttc tggcaaatat
     6061 gaaatccatc aatatcagga agcataatat ctaaaatagc catatccaaa tctttagatt
     6121 caatacattc tagagcttcc ttacctgtat aaaacttgta tactgtaaaa ccttcatttt
     6181 ttaaatatac ttcaattaaa tctgctattt ctttttcatc atccacaatc aaaatatttt
     6241 cgttcataga ttatttttca ctctccttat tatcgaggtc atcatttata atattggtaa
     6301 atttacatat caaataatta caagcatatt tgtaactaat ttttataaga aaagaactac
     6361 tacatcagaa tgtgttgatg ctactattgg aacacaaaaa ctgagattaa ttgttattat
     6421 aaaaactccc ctaacacgtc cttattatcg cataacaggt attaactttc aataaaaata
     6481 tctatcagtc aaacacaatt caatttatta taccagagac aatccatata aaaaacaatt
     6541 aaacctatgt atgtaaccac agacattact aaaatttcca tgttatttta tataaaaatt
     6601 caaaaaagga gttgaattta aaatttgatt agaaaaaaat caattttgag acaactcctt
     6661 tttattttaa tgtatttaaa ttatagtata tcagccttca taatatcctc aaaatcacct
     6721 ttttcagata ttaatcttat caatttaaca gtacagctta aatcatttat aagtatagct
     6781 ccaactaatg aaccatcttt cataaataat tttttatata ttctttgacc ttcatctttt
     6841 gatgaaattt ctttatcaaa attttgtata ttaccaatac taaatacttt tacattcatt
     6901 gcatctaatg aaactggata tacttcactt tcaaatgtca aattatctcc acttgcattt
     6961 gctcctgcta tttttcccat ttctactgaa gatggccata ttgctaaact aatatttccc
     7021 acttgtgcaa catctccaca agcatatata tctttaacat ttgtctgtaa tgtcttatct
     7081 acaacaatac ctctatcaat ttttacagat gtatcctgaa ccatttgagt gtttgctctt
     7141 acaccaattg aaaatactac catatcacag tcaacactat ctccatcttt aaatacaact
     7201 ttttgagctt ttccatcacc ttcaatacca tcaactgcta cacccaatct tacatctatt
     7261 cctgtatctc ttacacattg ttctaatatt ttactaccct ctttatcaag ttgttttgat
     7321 agtatgctat ccatggcttc aactactact acttctaatc cttttagtct aaattcccat
     7381 gcagcttcaa gtcccaataa accgccacca atcactacaa ctttcttaga tttttctagt
     7441 gcttctttta cactatataa atcttttata tttctaagag taaacacatt ttctaaatca
     7501 tggcccttaa tcggtggtat aaagtttcta cttcctgtag ctattacaag cttatcaaat
     7561 ttaatagctc catcattcac tataatagtt ttattcactt catctaattt atctacattc
     7621 gttcctaata ttactactat attattttta tcataccaat ctttatcttc catgtagaaa
     7681 tctgaaccaa gttcttcatt tataccatca gaaagtgcag gtctatagta agtcaactca
     7741 tcttcattag aaatcatagt tatcttacaa gttttatttc tttttcttat agcatctgct
     7801 gcataaaaac cagctgctcc atttcctact attacgaaat attcatcagt atcttttcta
     7861 aaagtaactt catcttcttc aacttctata aattggtctt ccttagctcc acatacaggg
     7921 catacactag gagcactatc accttcaaaa acttcattac aaattacaca tttaaatttc
     7981 ttagttttct ttttacttcc aaaagtagaa tttaacttat ctaggaattt acctgtataa
     8041 gaatgtaatt tatcaatctc ttcatttgat ggtttaaagt taacagcaaa aggttttact
     8101 gctgtcattc taagctgact tattctctcc atagcatttt ctataccttc tccactccat
     8161 ccatatgaac caaatactga agcaactttt ccaccatgaa gaactgggtt taacgatatt
     8221 aatatatccc atataggctt taacgcatct cctaatattg ttggtgttcc aaataataca
     8281 ccttgtgata ctgctatttt tgccaaaata tcttcttgct tatgctctat tacatcaaat
     8341 aaatttactt tatagttgct atttttttca atatatgctt ttatagcttc tgccataatc
     8401 tttgtatatc catgtgcaga aacataacaa attgtaactt ctttttctaa ctttatttgt
     8461 tcctttacac tccagttata ataaaggtct ataatttttc taggattttc agttaatact
     8521 ggaccatgac ctggacatac agtatctata tctaaatctt ttatcttttc tatagcagtt
     8581 accatagatg gtttaaatgg acccattata caatcataat agtatcttaa agcatccaag
     8641 taatcctttt cttcattttc atcaagtgta tttactatct tatcattact atagtgacta
     8701 ccaaatgaat cacatgttac aagagtttta tcctctttta tatatgtgta tatagtatct
     8761 ggccaatgaa gcataggtac agagaaaaat tctaaagttt tgtccccaat tgataatgta
     8821 tctccatctg taactgcaac atattcaaag tctttgttta cgatttcctt aagatattta
     8881 attgctgttt ctgaagctac tacttttgca tttttagcta aatccaaaag cttttcaacg
     8941 cttcctgcat ggtctggttc agtatgactt acaacaatat agtctatttt ttcaaaatct
     9001 atatttaaat catttaatct ttcaatataa ttgtcaaaat gcttatcttt aactgtctca
     9061 aacaaaactg ttttttctgt tccttttaat acatatgaat tataagtagt tccataaggt
     9121 gtatacataa ttatgtcaaa tactcttaaa tcagggtcaa gtgaacctac ccaatataaa
     9181 ttttctttta tttttagtga tttcaatgac ataccgtact cctcctaaaa tagttattta
     9241 gtctctttat accctgcttg ttatatttta aacaaaattt attcaaaaaa gaaattattt
     9301 taaagatagt ttaaatcttc taatcttctt tttctacctt caatacagtt ccctttttag
     9361 aatctacttt tatgtcatat tcatttttat ctttatctat tcttatttta taaaaagtaa
     9421 catctaaatc tttgtctaaa ctccattctt taaccattcc attttgttct tttaatgcaa
     9481 tttccatagc ttgttgaggt gtaattatgt catttaaatc taatttttct tttctagcaa
     9541 ctccatttaa atcttctgaa tctaactttt ctgttttatc ttctaataca tcttttgtat
     9601 tagcatcaat tttcatctta tattcattat tatcatctac accttccaca gtataaacaa
     9661 atttatttaa atctctctca agacttaaat ctactatatc agcatccttg tatttatctt
     9721 taaatatgtt tattgcatct gtgtatggca ctttaaaatc ttgttccatt aaattttcat
     9781 tatttttttc atcttttaaa gcattatttg aagcatttgc ctcctttttt gtattatctt
     9841 gtgcttgtcc tgaatcctta gttgggctcc cactacatgc agttaataaa cctgctataa
     9901 gtatacttac cactaataaa tacgtttttt tacacataaa aatcctcctt aaatatatta
     9961 atttattcta tattatgccc tctaaaccct ttatttaccc ttttatatta aaaattcctc
    10021 ctattttatc aattttaaaa ctttatattt actaaaatta aagttacata aaaattatac
    10081 tgtctcaaaa tagaaacaag catctttttc attgttgaaa taataaaaat atattttatt
    10141 tcgtacaaaa agagtgcctc atgaactaaa aagttcattt tgagacactc ccattctttt
    10201 atatttttac ttacaaaata acagtactta ttaatttttt acaaacctta taagtatatc
    10261 tatattatat tcttaaattt aaacactata ttaaaaaatt aaattttgtt taattctttt
    10321 actgttaaat ccattttagg atatggaatc gtaattcctt cttcgtcaaa cctaacttta
    10381 acctcttcta gtaaatcaag ataaattgtc caataatctt ctgtcttgca ccaaactcta
    10441 gttgcaaatt taacagcatt atctccatgt tctactactc ctataaatgg ttctggctca
    10501 tttattatta atttatgatt attaacaata tctttaagta ctcttctaac atgtagtata
    10561 tcatcttcat accctacact aaaagtcaaa tcaactcgtc tagtattctt tgctgaatag
    10621 tttattagac tatcatttga tacagaacca ttaggtataa gtatctgttt attatctgga
    10681 gtaaccaatt gtgtataaaa aagtcctatt tgttcaaccg ttcctccatg gcctgctgct
    10741 tctacgtaat ctcctacttt aaaaggtcta atcaatagta taataaatcc tccagcaaaa
    10801 tttgatagac taccttgtaa agccaaacct attgctaccc ctgctgatgc tatcacagca
    10861 gcaaaactgg acaaactcat tccccaatat tccagaacaa tgattataac ggcaacttta
    10921 agtacactca aaagaagaga cttcaaaaac cttcttaaag ttaaatcaat atctctttta
    10981 ctaagtacta acataacatg gtttacaaat ttttttatta ttttaaatcc aatagaaagt
    11041 ataagtatac ctattataag ttttactccg cttgtagtag cccattctag cagactttcc
    11101 attatacctc ccatatccat cttattaatg tctaaattat ttaaatctaa cttgcttata
    11161 tcatctattt tcgtcgtatc cataaaaaac tccttacctt atattttaga accaatatta
    11221 tagtttaaag ttcttatttt ctatttaata taatacaata ccatttgtac atagtaaaca
    11281 agatttatat tccaattata catcttagga aaatgcaaac tatttgttat atatatgcaa
    11341 aatataatac taaattaaac tattaataaa acactatata gccttacaat catcattttt
    11401 tacccctata tgagatgata ctatagttat aatacttagt actataaata ctacaaaacc
    11461 ttttttatat ccatcaacta tattatatga atttacttgt aaagagatag ctataatcaa
    11521 attaataatt ccacttccta taaattcaaa agtattaata acactagttg ccagtccaga
    11581 ataaatttct ttatttttat acttggcatc attaaaaacc tgtagatgtg acataactat
    11641 acatcccata ataaagaatg atattggtaa aactataatt ggtggtttta cctggtatac
    11701 aactacaatt acaatccata taaacaaatc tatcattgct ccaaatttta ttatattaaa
    11761 tttgctagac cttattttag caaatacaaa atccataatt attgaaccaa atataaatcc
    11821 atatgtaaaa aatgatacta taaacgcaga aaaactctta ctcacaccat aaacgtccat
    11881 tatgtatctt actccccaca agcttataaa tgcacttgta agaccaacaa aagagaacat
    11941 tatcattgaa ttataccatg ttgatttgtt ctttataaca gacttaatac catcaacaat
    12001 attaaccttc tctgactttt catagggttc agtatctaca ttgaatccat actcttttgg
    12061 tgtatctcta acaataatat atataaaaca tccaattaca acaccaatta taccgattaa
    12121 taaaaatgaa tttctccaac caacaagttc agacaaaaac acaagtggaa aagtagcaag
    12181 aactccacct aaatttccta caaatgaaaa tttagctgtt gcagaagcaa attctgactt
    12241 attaaaccat ctcccttgaa tttttaaaat acaaagtaat attactgatg ttcctgcccc
    12301 tacaattaca cgagaaatat atgctaattc tatactttga atcaaaccaa ataaaattga
    12361 ccctaaactt gccattatta ttccaagact gctaattttt cttgctccat atttatcaat
    12421 caaaattcct gcaggtattt gcataaatgc atatgcatat aggcaaaagg atgctatatt
    12481 tgaaatttgt atagaattaa aacctaactc atttgctaaa ttgtctgaaa caacagcagt
    12541 tgacatcctt aaaaataata ctaaaacaaa tgaaaatgta attatacccc aaacactcca
    12601 cttagccttt gtataacttt tcatatcttc tccctcaaat ccatatattt ttaaattttc
    12661 ttgatataac ttatataaag tgtatagtta ttcttacaaa tttttacacg caatatcatt
    12721 atacaacata tgtatatatt taactagaaa taaaatacta ttcatattat atacatgaat
    12781 taactacata aataataaga gccatctttt aacattactc aaaagatggc tccatataaa
    12841 aacatttatt ctaaatttaa ttttttatct aatataaatt ttgtagcgaa aaataacgct
    12901 attgcaacta caagactacc aagtattgca agcataaaac taggactctg ataaagatga
    12961 tataccatat ttgtatcttc atttggtaag atgcttccaa ctgcatctcc aacaattgat
    13021 attactatat ttatagctat aaaagaagca aatgccacta tgtttctatg tttattgaat
    13081 tttggtaatt gtcccattga gattgatgta tataataaca aaataaatga tgaatatgaa
    13141 ataaacatta ctactactaa taataaaata tctaaaaagt tttcagaaat ccatttaaaa
    13201 ctagtgttaa acaaatctaa aatttcaggt agtaaaattc cagaagtacc aaaaagcata
    13261 agaactccaa aagtgaatac tgcaactata aaactaagta cagcgtatat aagtgctgtt
    13321 atacatttag ataatataag tgaactagtg ctaacaggaa gagtaaacat taaataacct
    13381 tcatccccca aaagattttt tctaaacctt tgaattgtaa ctacaatagt taaaactcct
    13441 aatgccataa aaagacttgt taatactagt attcctattc cctgtacctg aagtatattt
    13501 gtattcataa atattccatt aaatacagct actatcaata tggctatata taacggtata
    13561 aatattcttc cacttgcttt taactcatat tttaataatt tacctaacat ttaaattcct
    13621 ccctaaataa tgcatctata gattttcctt tttcttctct tattgcttca acgtctcctt
    13681 gtaacactat ttctcctttt gatatgaata ttatctcatc acatatgttt tctatttcac
    13741 ttattaagtg ggtagcaatc agtaaagtac tatcttcaga ataatttttt aatatagtct
    13801 taagtatata tgaccttgca gctgggtcaa caccacctat aggttcatcc aatatatata
    13861 tgctcgcatt tcttgacatt actaaaataa gttgaacttt ttctttagtt cctttagaca
    13921 ttgtctttag cttttcattt acatcaatat ctaagctttt aatcatctca tttgctcttc
    13981 taacatcaaa atcactataa aaatcataga aaaacttaag taaatctgat accttcatcc
    14041 aatcatttaa ataagttctt tctggtaaat atgaaactat ttttttagta tctatggatg
    14101 gtttcattcc atttatcata atttcaccat tatcaggctg taataatcca ttcattagct
    14161 taatcattgt acttttacca cttccattag gccctaacag cccgactatt tttccctttg
    14221 gtatatttaa atcaatattt tttaaaacat tcttagttcc ataactcttg tttacacctt
    14281 taaattcaac tattccatct tgagaaacat tgcttatatt tttatccatt ttataattct
    14341 cctttcagat tttctgtaat aagttttagc atttcatctc tatcatatcc aagcttttct
    14401 aaggttactt ttaatgtatt tatctccaca ttagctattt cctctttcat agcttttatt
    14461 ttttccttat catctgttac aaatcttcct ttagttctct gactataaac taaaccttgt
    14521 ctttcaagtt ctgttagagc cttttgcatt gtatttggat taacttccgc atcttctgct
    14581 agtgctctca ctgtttctaa tttagatcct attttaattt cccctgaaat tatttttaac
    14641 tttaaatgtt ctactaattg tatatatata ggtttgttat tatcaagttc ccattccatg
    14701 ttcatgcctc cttattatat t
//
LOCUS       DAMJWP010000082        14677 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.086, whole genome shotgun sequence.
ACCESSION   DAMJWP010000082 DAMJWP010000000
VERSION     DAMJWP010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14677
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.086"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            370..1281
                     /locus_tag="PC700_002021"
     CDS             370..1281
                     /locus_tag="PC700_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HDF2581332.1"
                     /translation="MKINRLTEIIVILLNKKLVTAKELADRFEVSTRTIYRDIETLSM
                     SGVPVYMTKGKGGGISLIEEYSIDKAILSKKDKESLIVALKTLQATKYPEINSVVNKI
                     GSIFGEQNFSNWIEIDFTEWGSNFNEDDKFTKIKEAILRRNTINFNYVNSLSSQTNRT
                     VEPLKLMYKSKTWYLYGFCKLKDDFRIFRISRIRNLSIKDEVFSRKIIEEVCLNDSKV
                     IKENTITLKLRFKEKMLFRVFDDFNKDLITKNEDDTYDVITEFPIGEWIYGYILSFGD
                     NVEVLEPKDVRDNVITRLRELSKIYSL"
     gene            1456..1953
                     /locus_tag="PC700_002022"
     CDS             1456..1953
                     /locus_tag="PC700_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HDF2581333.1"
                     /translation="MNYEIVEINEKIVIGISKETTNKDGQAVNAIGELWKKFMGKGIY
                     NAIKGKKNDKTIGLYTDYQGDFTSPYSFVACCEVNSNSDKEKNLEELNTNNTVGESII
                     SKVILAGKYAKFVIVGGQKEVGDFWFKFWQMDFDRTYISDFEEYQCNTFDTKKQEIHI
                     YIGIK"
     gene            2697..3059
                     /locus_tag="PC700_002023"
     CDS             2697..3059
                     /locus_tag="PC700_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDF2581334.1"
                     /translation="MENKILIVDDAIFMRMIIKEALSKSGYNNLIEANDGEEACNIFK
                     YEKPDLMLLDITMPKKDGLEVLREIKKLDSSAKVVMCSAIGQENMIVEAIKLGALDFI
                     VKPFKTETLVKVVNNVLK"
     gene            3125..3742
                     /locus_tag="PC700_002024"
     CDS             3125..3742
                     /locus_tag="PC700_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HDF2581335.1"
                     /translation="MINYLELDDVHIDALREIGNIGSGNAITALASMINSNVEVLIPM
                     VKILEYNEATNLLGGPENKVVCILLDMKGDINGMFMFLLDESITQLMLSSLFNKEEAF
                     LDEIEAIEISAIKEIGNIMASSYVNAIASMLNMTISVSIPDICIDMVGAVLNVPMIRF
                     SDVGDKVLFIENKFKMSDNYFTSHILMIPEMSSLREILVRLGLEV"
     gene            3739..4224
                     /locus_tag="PC700_002025"
     CDS             3739..4224
                     /locus_tag="PC700_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HDF2581336.1"
                     /translation="MNREIVVNIADMKIAYRPNVLVTYALGSCVGVCLIDKVAGIGGM
                     LHVMLPYSKDAINIENKCKFADTGINELIKSMENIGANRIYIKAKIAGGAQMFLGSSN
                     SVIASIGHRNVEAVKKTLLGLNIPILAEDTGMNYGRTIRFYTETGELLVDSINKGKKK
                     L"
     gene            4267..4785
                     /locus_tag="PC700_002026"
     CDS             4267..4785
                     /locus_tag="PC700_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-binding chemotaxis protein CheW"
                     /protein_id="HDF2581337.1"
                     /translation="MRFPDDPYLDYGLIDEGIKYLKFKVDEQDFGVELEKVIEITGNQ
                     EIIFIPELPTYSKGIINLRGKIIPIIDMRLRLKKDELMTTNCMYIVVTEIKDLVVGFV
                     VDRVDDIVSLEKSKISPPPRVTIEYSDYFVSGVGELEGKIITLLDLEKLLTDKDEVLI
                     DELINGFDCTIM"
     gene            4921..5880
                     /locus_tag="PC700_002027"
     CDS             4921..5880
                     /locus_tag="PC700_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2581338.1"
                     /translation="MKNKQQGTILVVDDSVLMCDLIDKTLTKDNFKVKKAFNAIEAKE
                     FIEEFIPDIILLDIILPDNSGFEFCKEVKSNTRTADIPIIFITSKNTDTDIVKGFGVG
                     AIDYMVKPFSMTELKARVIAHLEVKKSQDKLKKINNELESSLEKLNRLVVRDYLTGLY
                     NRRHIINQLMEQRRINKYSETSISLILGDIDDFKVINDTYGHEAGDFVLTVISSIIKK
                     NCRQIDTVSRWGGEEFLIMLTNTPIEGAKILSERIRKEIKEFDFNYKEHKIKCTITLG
                     VVEVNSNISLEENISLADKAMYEGKNLGKDCSVVSTMKGLKKI"
     gene            5906..7609
                     /locus_tag="PC700_002028"
     CDS             5906..7609
                     /locus_tag="PC700_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HDF2581339.1"
                     /translation="MKREVKLSKLAIVLLIIIVLAVLAGIFSSISSYKAFEKVEKSIN
                     DMNNLRTQSQYIKDTTREISDVLRRYVFTGEKKNKDIYEKELYGKTRESALNKLEKIG
                     LTKSESNKILEAKEISDSLVPTEQSAIKAVESNDKSKAQSIIFGSDYINSQEKMDSLI
                     NEFQEDIVTRSNSKIVEVKKELQSLILRTIFILSLLIILTIIEYTVIKLKIVIPINRV
                     EKHFSRIASGDLRTSIDVEESKSEIGLLVSSVKKMQSMLLNYIDLIDSTLTSIAKGDL
                     RVEIDQEFIGDFKSIKISLESIINSFNGDFSEINLSAEQVASASEQVAAGAQALSQGA
                     TEQASSVEELASTINEISDGIKKCADSASLACEVSDQSASQVGFGSRCMDDMMTSMGE
                     ISEKSSEISKIIKTIDEIAFQTNILALNAAVEAARAGQYGRGFAVVADEVRSLAGKSA
                     KAAQNTAILIEEAIKTVENGVRIAKETANALDLVVNGVEKTTNYINGISSAVHSQEVA
                     ITQILSGIEQISIVVQMNSATAEESAAASEEMSAQAQILKELVEKFHLKESEVRESSW
                     V"
     gene            7866..9968
                     /locus_tag="PC700_002029"
     CDS             7866..9968
                     /locus_tag="PC700_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HDF2581340.1"
                     /translation="MRQMLDMFIFETSLLLEQLDEIMIQIEKEKVFTHNNINETFRIM
                     HTIKGSASMMGLDNISALAHSVEDLFYAIREGKIEVLDNDLLFDLVFQSLDFIRRDID
                     ELQSRGCISSDYSNFIEGIEEYVSTLELSSSMKSKDNANLESTDEKEAIDSNFEYKED
                     SDVVKIIFEQGCMMENVRAFMLVFELRDYCEFIDFYPKDVETNAESTEYIKSYGFYIQ
                     FVAKEDKAIVYKTIEDNLYVKEYINISKKEIEMKNTSNNDAVENKLKDNNKVSKSYQN
                     NYTEDEIQEDVLNEKTDMEKDSLSEENEHLPISKQSIMSVNLEKLDLLQNIVGEIVIM
                     ESMVVNSVNSTGVDIDNFNKATRQLHKLTDELQDIVMSMRMIQIAGVFQKMHRIVRDM
                     NKKLDKNVELITIGAETEVDKNVIDNLAEPFMHLIRNAMDHAIEPIEERIAKGKEEVG
                     KIVLSAENLGGEAVISISDDGRGIMRDKILAKAKEQGLLKHQINEYTDDEVNSLIMTP
                     GFSTKESITEFSGRGVGMDIVRKSIEKVGGSIEVKSKQDEGTTFTIKIPLTLSIVEGM
                     KFKVGDSLFTLPISSIRRTFKLTDLNQIITEGNKTELITIYGACIPIIRLHKIYNIQT
                     KVTNLMHGNMIIIENEHKAVCIFVDEIIGNQQVVVKPFPVYFNRFNIKNKGLSGCTIL
                     GDGSISLIIDADSIIEIY"
     gene            9981..10421
                     /locus_tag="PC700_002030"
     CDS             9981..10421
                     /locus_tag="PC700_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HDF2581341.1"
                     /translation="MAKDEIIKQVLTFYVNDVIYGIELENVIETIRFQSITYVPCLPT
                     YISGVINLRGRIIPVINMHIKYNLPKADYNERTCIIITKVDEYQVGIIVDKVVDVIHI
                     ENLNLLETTNSNNTNINKDIKEIAKVEDNSILILDIRKFLINSI"
     gene            10457..11260
                     /locus_tag="PC700_002031"
     CDS             10457..11260
                     /locus_tag="PC700_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HDF2581342.1"
                     /translation="MIKLTDEEFIVLVNHVKKEYGIDLSKKRALIEGRLYNTMIEKKL
                     SSFSQYMNLLFKDKTGNEAINLINRLSTNHTFFMREPQHFEFIQNSILPFWEENNKIR
                     NINIWSAGCSSGEEAYSIAMTLDDYFGYNKELWNIKIIATDISTNSLEKAKRGIYIES
                     NVNNVPELWKKKYFIDNKDGTFKICDKIRKSVIFKSFNLMNDFSYQHFDLIFCRNVMI
                     YFDLKTREELINKFYNATKKDSFLFVGHAEVINRSSTKYKYVKPAIYKR"
     gene            11300..11896
                     /locus_tag="PC700_002032"
     CDS             11300..11896
                     /locus_tag="PC700_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HDF2581343.1"
                     /translation="MERFNNMVIAIGASVGGTEAILEIIKDLPKSTPGIVVVQHMPAI
                     FTYMYAQRLDKQCIMNVREAKNNDRVEQGNVLIAPGGYQMKLCTDKQGYYVTCEKGER
                     ISGHCPSVDVLFDSVAEVAGKNSIGIILTGMGYDGANGLLKMKENGLFTIGQDENSCI
                     VYGMPMVAFDRGSVMLQLPLGEISNCLIRHINILKQKD"
     gene            12002..12865
                     /locus_tag="PC700_002033"
     CDS             12002..12865
                     /locus_tag="PC700_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HDF2581344.1"
                     /translation="MKFFKVDTKAKVLRYVFVLTLTIMAVLAIKHDYKYNNLRKDASI
                     NLNSNKEAVTVMSNQSLPKGNTNSKQTLSVSTIGSKTIVKNDEYLESTINMPVITNSN
                     KIVERSTNDKIKNDIFEFYNKSYKEAKQYLKDNPDEKNKFVANVDFELKKNTDSALSI
                     KVRYYTYSGGAHGFYQDIAYNVDMRTGKFLELMDLFKDNTKYKEVIDEEIKRQIAELE
                     KKDEENIGIYNFKGIKENQNFYLQDENLVVYFDLYDITPYAAGIPEFSINKALISSML
                     KPEYVDLFDLK"
     gene            12932..13921
                     /locus_tag="PC700_002034"
     CDS             12932..13921
                     /locus_tag="PC700_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HDF2581345.1"
                     /translation="MLLLKKDDIKKVFTMRDAIEADKEAFRIYCEGKSVNPLRTNISV
                     PSQDASMLFMPGYVEELGCGGIKIVSVFPKNAQKGKPVIPATVLLLDGETGEVSAVLD
                     GTYVTQIRTGAASGAAIDVLAKKDSKIGALIGLGGQGEPQLEAMIEARNLDEVRVFSR
                     NRESREKFAEEMNLKLSKYNTKIVAVESSDEAIDNADVIVLATPSKQPVLNGNLVKKG
                     ALISAVGSYMPDMQELCPNCLTRASKIFFESTDAVLSESGDILIPLKEGKISKDDFSG
                     DLGNVINGTIPGREDDDEIIVFKTVGIGVQDVVTAKRIYDKAKENGIGIDWKW"
     gene            14013..14624
                     /locus_tag="PC700_002035"
     CDS             14013..14624
                     /locus_tag="PC700_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2581346.1"
                     /translation="MKMVKKLILSTIMALVLVLTIGCSNAEEKVKSNFEANLAALEMY
                     KYDEKGISDALTEAEYKQIQTYNSKDKVSMIAQLTPLYGKENAEKIYETIVKLIQGVE
                     KTVTVESSSKDKITLKVVSKALDTSKVMSEANTLVKSYVTDEKNYDKKEKEVQDDMTK
                     MIIDLYNKKPTKEVTTMITYTKKDGKWQPPSNIQKLCEGIYTI"
BASE COUNT         6001 a         1381 c         2723 g         4572 t
ORIGIN      
        1 gaaatgataa taagtgaatt gatatagcaa taagtaaatt aagtaagacg ataacaaatg
       61 aaatgataat aagtgaattg atatagcaat aagtaaatta agtaagacga taacaaatga
      121 gatgataata agtgaattga tatagaaata agtaaattaa gtaagatgat aacaaatgag
      181 atgataataa gtaaattgat atagtaataa ataaattaag atgataatat ccaaagtgag
      241 aaagataata gaaagtaaat aaaatcatgt aagaataatt tagataagat tctaagattt
      301 tatttattta aaaattagta tgattaattt agattaaata aatttaataa ttttagtagg
      361 tggtaatgaa tgaaaataaa tagattaaca gaaataatag taattctatt aaataaaaaa
      421 ttagtaacag ctaaagagtt ggcagatagg tttgaagttt ctacaagaac catatataga
      481 gatatagaaa ctctgtctat gtcgggagta ccagtatata tgacgaaagg caaaggagga
      541 gggatatctt taatagaaga atattctata gacaaggcca tactgtcaaa aaaagataaa
      601 gagagtttaa tagttgcact gaaaacatta caggcgacta agtatccaga aataaattca
      661 gtagtaaata aaattggctc aatatttgga gagcagaatt tttcaaactg gattgaaata
      721 gactttactg aatggggaag taattttaat gaagatgata aatttacaaa aataaaagaa
      781 gctattttaa gacgtaatac tataaatttt aattatgtaa atagtttgtc aagccagaca
      841 aacagaactg tcgaaccact gaagcttatg tataagagta agacttggta cttgtatgga
      901 ttttgtaaac taaaagatga ttttaggata tttagaatta gtaggataag gaatttatct
      961 ataaaggatg aagtattttc aagaaagata atagaagaag tatgtttgaa tgattctaaa
     1021 gtaattaagg aaaatactat aacgttgaaa ttgaggttta aagaaaagat gctttttaga
     1081 gtatttgatg attttaataa ggatttaata actaaaaatg aagatgatac atatgatgta
     1141 attacagaat ttcctattgg tgaatggatt tatggatata ttttatcgtt tggtgacaat
     1201 gttgaagtac ttgaacctaa agatgttagg gataatgtaa taactagact aagggagttg
     1261 tctaaaattt attcattata atatgatgct agattaaaga ttttattaaa tatataatat
     1321 cttgcattta gtatataata tctcactgag tttaattata ttaaaaatat gacataaagg
     1381 tgtcgtatat tataaattat aattgaatta gttcaaaata aaatatatta ataaaaacaa
     1441 agtgaggtat aaagtatgaa ttatgaaata gtagagataa atgagaaaat agttatagga
     1501 atatcgaagg agactacaaa taaagatgga caagcggtaa atgctatagg tgaattgtgg
     1561 aaaaaattta tgggcaaagg gatatataat gctataaagg gtaaaaagaa tgacaagaca
     1621 ataggtctat atacagatta ccagggtgat tttacaagtc cgtatagctt tgttgcatgt
     1681 tgtgaagtaa atagtaattc tgataaagag aagaatttag aagaattaaa cactaataat
     1741 actgttggag aaagtattat tagtaaagtt atactagcag gtaagtatgc aaagtttgtt
     1801 atagtaggag gtcaaaaaga agtaggagat ttttggttta agttttggca aatggatttt
     1861 gataggactt atataagtga ttttgaagag tatcaatgta atacttttga tacaaagaaa
     1921 caagaaattc atatttacat aggaataaag tagatttgtt attactatat tgaatgaagt
     1981 attgagtata gtacatttat tatagatagt tttactattt ataactataa gcagtaaatc
     2041 tgtaaaagat ttactgcttt tttagtaatt aatagtatca gtatcacttt gaatcttcag
     2101 acatattcgt catttaatat tgtttattga taaatgatac ttgtaaatat aatgatttgt
     2161 tatttaattt aaaatatgaa aaaaagtatt ataaacacat tagaatactt ctggatttat
     2221 gagtatacaa tttgaaaaat ataaaaaata atgtataaag ctaaatctaa tttaatcgat
     2281 aatacatata gagaaatata attaagtaaa taaacaacta tctattagat attgagaaag
     2341 tcaaatccta tatgaatcta cagaaatgat tttaatgaat ctgaaaaaat tttttataca
     2401 aacataaaaa aggtataaag ttgttttatt ataatttgat tacttttgat aactttttat
     2461 gttttataca attatatttt taataactcg tcaaaacgcc aaacatatat tacaatagat
     2521 atataagttt ttttgttgac aaattatgtt aagagaatta agtataatat ggcataaata
     2581 attactttta tttatcaatg aaatgtattt attgtgtagc gaaaaatagt cagatagtat
     2641 agacaataac tgaagataaa agcgtttatt gttgtacata tggaggagtt agttttatgg
     2701 aaaataaaat tttgatagta gatgatgcta tttttatgag aatgataatt aaagaagctc
     2761 tgagtaaaag tggctataat aatttaatag aagcaaatga tggagaagaa gcgtgtaata
     2821 tatttaaata tgaaaagcct gatttgatgt tgcttgatat tacaatgcca aaaaaagatg
     2881 gtttagaggt gctaagagaa ataaaaaagt tggattcaag tgctaaggtt gtaatgtgct
     2941 ctgcaatagg acaagagaat atgattgtag aagccataaa acttggagcg ttagatttta
     3001 tagtgaagcc cttcaaaaca gaaacattag ttaaagttgt aaataatgtt ttaaaataaa
     3061 agtgtaaata tacactgata caatgaaaag caaataaatg caataataaa agggaggggt
     3121 ttatattata aattatttag aacttgatga tgttcatata gatgctctta gagaaattgg
     3181 taatataggc tctgggaatg caattactgc acttgcatcc atgataaata gcaatgtaga
     3241 agtattaata cctatggtca agatacttga atataatgaa gcgacaaatt tgcttggtgg
     3301 tccagaaaac aaagtagtat gtattttact cgatatgaaa ggtgatatca atggaatgtt
     3361 tatgtttttg ttggatgaat caataactca acttatgcta agttctcttt ttaataaaga
     3421 agaagctttt ttagatgaga ttgaggcaat tgaaatttca gcaattaagg aaataggaaa
     3481 tattatggca agttcatatg taaatgctat tgcatctatg ttaaacatga ctataagtgt
     3541 ttcaattcca gatatatgta ttgatatggt tggagctgtt ctaaatgtgc ctatgataag
     3601 attttcagat gttggtgaca aagtcttatt tatagaaaat aaatttaaaa tgagtgataa
     3661 ttattttact agccacattt tgatgattcc agaaatgagc tcacttagag aaatactcgt
     3721 gaggttggga ttagaagtat gaatagagag atagtagtga acattgcaga tatgaagata
     3781 gcctatagac caaatgtttt agttacttat gcacttggct cttgtgtagg agtatgtttg
     3841 atagataaag tagctggaat tggtggaatg ttacacgtta tgttgccata tagtaaagat
     3901 gctattaaca ttgaaaataa gtgtaaattt gctgacacag gaataaatga gctcattaaa
     3961 tctatggaga atataggtgc aaatagaata tatataaaag caaaaattgc tggaggtgct
     4021 cagatgtttt taggaagcag caattcagta attgcaagta taggacatag gaatgttgag
     4081 gcagtaaaaa aaactctttt aggattaaat attccaatat tagctgagga cacaggtatg
     4141 aattatggta gaacaatacg tttttacact gagacgggtg aattacttgt agattcaata
     4201 aataaaggta agaagaaatt ataatatata tttcatagaa agtgtatttt aggagggagg
     4261 tattaaatga gatttccaga tgacccatat ttagattatg gactaattga tgaaggaatt
     4321 aagtatctaa agtttaaagt agatgagcaa gattttgggg ttgaacttga aaaggtaata
     4381 gaaattactg gaaatcaaga aattatattt attccagaac tacctacata ttctaaaggc
     4441 ataataaatc taagaggaaa aatcattcca ataatagata tgcgtttgag acttaaaaaa
     4501 gatgaattaa tgactacaaa ttgcatgtat atagtagtaa ctgaaataaa ggacttggta
     4561 gttggatttg tggttgatag agttgatgat atagtaagct tagaaaaaag taaaatatca
     4621 cctccaccaa gagttactat agaatattcg gattattttg taagtggcgt tggagaatta
     4681 gaaggtaaaa taattacttt actagattta gagaaacttt tgacagataa agatgaagtt
     4741 ttaatagatg aattaatcaa tgggtttgat tgcactatca tgtaacatag ggatgtagtt
     4801 ggagtgttat tataaaaaga ttgtgatgag aacatttaag aatatagtgg tataattatt
     4861 aagtataaaa attttaaatc attaaaattt gaatatatta tgtttaagag aggtttgtat
     4921 atgaaaaata agcaacaggg aacaatttta gtcgttgatg atagtgttct gatgtgtgac
     4981 ttaatagata aaacacttac aaaagataat tttaaggtta aaaaagcatt taatgcaata
     5041 gaggcaaaag aatttattga agaatttata ccagacatta ttttgcttga tataatttta
     5101 ccagataact ctggttttga gttttgtaag gaagtaaaat ctaatactcg tacagcagat
     5161 atacctataa tttttataac ttcaaaaaat acagatacag acatagtaaa aggatttggt
     5221 gtaggagcaa ttgactatat ggtgaaacct tttagtatga cagaattaaa agctcgtgtt
     5281 atagctcatc ttgaagtaaa gaaatctcaa gataaattaa aaaagataaa taatgaactg
     5341 gaatcttctc ttgaaaaatt gaatagactc gttgtaagag attatcttac aggtctttac
     5401 aataggcgac atataattaa tcagcttatg gagcaaagaa gaattaataa atatagtgaa
     5461 acatcaatat cccttatcct tggtgatatt gatgacttta aggttatcaa tgatacttat
     5521 gggcatgaag caggggattt cgtgttaaca gtaatatcaa gtataataaa aaagaattgt
     5581 agacaaattg atactgtatc aagatggggt ggagaagagt ttttgataat gctcacaaat
     5641 actccaatag aaggtgctaa gatactttca gaaagaatcc gtaaagagat aaaggagttt
     5701 gatttcaact ataaagaaca taaaataaag tgcactatta cattaggagt agtagaagta
     5761 aactcaaata tatcattaga agaaaacata tccttagcag ataaagcaat gtatgagggt
     5821 aaaaacttag gaaaagattg tagcgtagtg agtacaatga aaggattaaa aaagatataa
     5881 acttacttta tgaaggagta cagatatgaa aagagaagta aagctttcta aattagcaat
     5941 tgtattgctg attattatag tattggctgt tctagcaggg attttttctt ctatttcatc
     6001 ttataaagca tttgaaaaag tagaaaaaag tattaatgat atgaataatt tgaggactca
     6061 aagtcagtat ataaaagata caactagaga gataagtgat gtattaagaa gatatgtatt
     6121 tacaggagaa aaaaagaata aagatattta tgaaaaagaa ttatacggaa aaacaagaga
     6181 atcagcttta aacaaattag aaaaaattgg tcttacaaag agcgagtcaa ataaaatctt
     6241 agaagcaaaa gaaatatcag attctttagt accaacagaa caaagtgcaa taaaggcagt
     6301 cgaaagtaat gataagagta aagctcaaag tataattttt ggtagtgact atataaactc
     6361 ccaagagaaa atggattctt taataaatga atttcaagag gatatagtta cccgttcaaa
     6421 ttccaaaatt gtagaagtaa aaaaagaatt acaaagtcta atattaagaa caatatttat
     6481 actttccctt ttaataatat tgacaataat agaatataca gtaataaaat taaagatagt
     6541 gattcctatt aatagagtag aaaaacactt ttcaagaatt gcatctgggg acttaagaac
     6601 aagtatagac gttgaagaaa gtaaatctga aataggacta ttagtgagtt ctgtcaaaaa
     6661 gatgcaaagt atgcttttaa attacataga ccttatagac agtacattaa ctagtattgc
     6721 aaaaggagat ttaagagtag agatagacca agaatttata ggtgatttta aatcaattaa
     6781 aatatcttta gaaagtataa taaactcttt taatggtgat ttttctgaaa taaatttatc
     6841 agcagaacaa gttgcaagtg cttcagaaca agttgcagca ggtgcacaag ctctttcaca
     6901 gggtgctact gaacaagcta gttcagtaga agaattagct tccacgatta atgagatatc
     6961 agatggaatt aagaagtgtg cagatagtgc tagtcttgca tgtgaggtat ctgaccaatc
     7021 tgcatcacaa gttggttttg gaagtagatg tatggatgat atgatgacat caatgggtga
     7081 aataagtgaa aaatcgagtg aaatatcaaa aataatcaaa acaatagatg agatagcatt
     7141 ccaaactaat attctcgcat taaatgcagc tgttgaagca gcaagagcag gtcaatatgg
     7201 tagaggattt gcagttgtag cagatgaagt taggtctttg gctggtaaaa gtgcaaaagc
     7261 agctcaaaat acagctattc tcatagaaga agctattaaa acagtagaaa atggtgtcag
     7321 aattgctaaa gagactgcaa atgcattgga cttggtagta aatggtgtag aaaaaactac
     7381 taattatatt aatggtattt cttcggctgt acatagtcaa gaagtagcaa ttacacagat
     7441 tttatctgga atagagcaaa tttccatagt agtccaaatg aactcagcaa cagcagagga
     7501 aagtgcagca gcctcagaag agatgtctgc tcaagctcaa attttaaaag aattagtaga
     7561 aaaatttcat ctaaaggaaa gtgaagtaag agaatcatca tgggtttaaa ttatgaggtg
     7621 aatattgaag taagagaatt atcatgtatt taaattatga ggtgaatatt gaagtaagag
     7681 aattatcatg tatttaaatt atgagatgta tatatattga aataagagaa tgattgtgta
     7741 tttaaattat gaggtacata tagtgagata agagaatcat caaggattta aattatgaga
     7801 tgcatatata atagaaaatt atttgtaggt cgtaggtgat aaatgtgaat tatattgaac
     7861 cgagtatgag acaaatgtta gatatgttta tatttgagac aagtttatta ctcgaacaat
     7921 tagatgaaat tatgattcaa atagaaaaag agaaagtatt tactcataac aacattaatg
     7981 aaacttttag aataatgcat acgataaaag gttcagcatc tatgatgggg ttagacaata
     8041 tatctgcttt agcacatagt gtagaagact tgttttatgc tatacgtgaa ggtaaaatag
     8101 aagttttaga caatgaccta ttatttgatt tagtctttca atcattagat tttataagaa
     8161 gagatataga tgagctacaa tcaagaggtt gtatatcttc tgattactct aattttattg
     8221 aaggaattga agagtatgtg agtactttgg aattatcaag ttctatgaaa tcaaaggata
     8281 atgcaaattt agaaagtaca gacgaaaaag aagcaatcga ttcaaatttt gaatacaaag
     8341 aagactctga tgtagtaaaa ataatctttg aacaaggttg catgatggaa aatgtgagag
     8401 cgttcatgct tgtatttgaa cttagagatt actgtgaatt tatagatttt tatccaaaag
     8461 atgtagaaac aaatgcagag tctacagaat atattaaaag ttatgggttt tatatacaat
     8521 ttgtagctaa agaggataag gcaatagttt ataaaactat agaagataat ctctatgtaa
     8581 aagaatacat aaatattagt aaaaaagaaa tagaaatgaa aaatacatca aataatgatg
     8641 cagtagaaaa taaattaaaa gataataata aagtaagtaa aagttatcaa aataactata
     8701 cagaagatga gatacaagaa gatgttttaa atgaaaaaac tgatatggaa aaagatagtc
     8761 taagtgaaga aaatgaacat ttaccaataa gtaagcagag tattatgagt gtaaatttag
     8821 aaaaattgga tttacttcaa aatatagttg gagaaattgt aattatggaa tcaatggttg
     8881 taaatagcgt aaattctaca ggtgttgata tagataattt caataaggca acaagacaat
     8941 tacacaagct tacagatgaa ttgcaagata tagtaatgtc tatgcgtatg attcaaattg
     9001 ctggtgtatt tcaaaaaatg catagaattg tacgagatat gaataaaaaa ttagataaaa
     9061 atgtagagtt aataactata ggagcagaga cagaggtaga taagaatgta attgataatt
     9121 tagcagaacc ttttatgcac cttataagaa atgctatgga ccatgcaata gagcctattg
     9181 aggagcgtat agcgaaaggt aaagaagaag tgggaaagat agttttatct gctgagaatc
     9241 taggcggaga agctgtgata agtatttcag atgatggtag aggcattatg agagataaga
     9301 ttttggcaaa agctaaggaa caaggtcttc ttaaacatca aattaatgag tatactgatg
     9361 acgaggtcaa ttctttgatt atgactccag gtttttctac aaaagaatct ataactgaat
     9421 tttctggaag aggagtaggc atggacattg ttcgtaaaag tattgaaaaa gttggaggtt
     9481 caattgaagt aaaaagtaaa caggatgaag ggacaacctt tactataaaa ataccattaa
     9541 ctctttctat agtagagggt atgaagttta aagttggaga ttcactattt actttaccaa
     9601 tatcatccat aagaagaaca tttaaactta cagacttaaa tcaaataatt acagaaggaa
     9661 ataagacaga gttaattaca atatatggag catgtattcc cattataagg ttacataaaa
     9721 tatataatat tcaaacaaaa gtaaccaatc tgatgcatgg taatatgata attattgaaa
     9781 atgagcataa agcagtatgc atttttgttg atgaaataat aggtaatcag caagttgttg
     9841 tgaaaccatt tccagtttat ttcaaccgat ttaatataaa aaataagggt ttatcgggat
     9901 gtacaatact tggggatgga agtataagtt taattataga tgcagatagc attatagaaa
     9961 tatattaggg gatttaaagt atggctaaag atgaaattat taaacaagta ttaacatttt
    10021 atgtaaatga tgttatttat ggaatagaat tagaaaatgt gattgaaacc ataagatttc
    10081 aatcaataac gtatgtacca tgtcttccaa catatataag tggagttatt aatttgagag
    10141 gaagaataat accagttatt aatatgcata taaagtacaa tcttcctaaa gctgattata
    10201 atgagagaac ttgtataata attacaaagg ttgatgagta tcaagttgga attattgtag
    10261 ataaagttgt tgatgttatt catatagaaa atctaaattt actagagacg actaattcta
    10321 acaacactaa tattaataaa gatataaaag agattgcgaa agttgaagat aattctatat
    10381 taattttaga tattcgaaag tttttgatta atagcatata gttagttctg ataaattagc
    10441 aggaggaagt ttttgcatga taaaactcac agatgaagaa tttatagttc ttgtaaacca
    10501 tgtaaaaaaa gaatatggta ttgatttaag taaaaaaaga gcgttaattg aaggtcgctt
    10561 atacaataca atgatagaga aaaaacttag ttcttttagt caatatatga atctattatt
    10621 taaagataag acaggaaatg aagctataaa cctaataaat agattgagta ctaaccacac
    10681 attttttatg agagaacctc aacattttga gtttatacag aatagtatac ttcctttttg
    10741 ggaagaaaat aataaaataa gaaacataaa tatatggagt gcaggatgtt ctagtggaga
    10801 agaagcgtat tcaatagcta tgactttgga tgattatttt ggatataata aagagttgtg
    10861 gaacattaaa atcatagcca cagatatttc aactaactca cttgaaaaag ctaaaagagg
    10921 tatatacata gaatcaaatg ttaacaatgt tcctgagtta tggaagaaga agtattttat
    10981 agacaacaag gatggtacat ttaaaatttg tgataagatt agaaaaagtg taatttttaa
    11041 gtcatttaat ttaatgaatg atttctcata tcaacatttt gatttgattt tttgtagaaa
    11101 tgttatgatt tattttgatt taaaaacaag agaagaactt ataaataaat tttataatgc
    11161 taccaaaaaa gatagttttt tgtttgtagg acatgcagaa gtaatcaaca gaagtagtac
    11221 taaatacaaa tatgtaaaac ctgctatata taaacgataa aaaggtattt ttgatatatg
    11281 attttttgga ggcggtaatg tggaacgttt taataatatg gttattgcta taggcgcatc
    11341 tgtaggtggt acagaagcaa tacttgaaat tataaaagac ctaccaaaat caacaccagg
    11401 gattgttgta gttcaacata tgccagctat atttacttat atgtatgcac aacggcttga
    11461 caagcagtgt attatgaatg taagagaagc taagaataat gatagagtcg aacaaggaaa
    11521 tgtgttaatt gcacctgggg gttaccagat gaaattgtgt acggataaac aaggatatta
    11581 tgtaacttgt gaaaaaggag aaagaataag tggtcactgt ccatcagtag acgttttgtt
    11641 tgattcagta gcagaagtag caggtaaaaa ttcaatagga ataatactta caggtatggg
    11701 ttatgatgga gcaaatggac ttttaaaaat gaaagaaaat ggtttattta caattggaca
    11761 ggatgaaaat tcttgtatag tatatggaat gccaatggta gcttttgata gaggttctgt
    11821 tatgttacaa ttacctcttg gtgaaatttc aaactgtttg ataagacata ttaatatttt
    11881 aaaacaaaaa gattaatttt ttataaataa aaattaatct tttattaaat tatatggata
    11941 aaaaacccta gaaactccaa atataataag agagatttat atttaggagg aaaatgataa
    12001 tatgaaattt tttaaagtcg atacaaaagc taaagtttta agatatgtat ttgttttaac
    12061 attgactata atggctgttt tggcaattaa acatgactat aaatataata atttaaggaa
    12121 agatgcttca attaatttaa atagtaataa ggaagcagtg actgttatga gtaaccagtc
    12181 attaccaaag ggtaatacaa atagtaaaca aactttatca gtaagtacaa taggaagtaa
    12241 aacaatagtg aaaaatgatg agtatttaga aagtactata aacatgcctg taataacaaa
    12301 ttcaaataaa attgttgaaa ggtctacaaa tgataagata aaaaatgaca tatttgaatt
    12361 ttataataaa tcatataaag aagcaaagca gtatcttaag gataatcctg acgagaaaaa
    12421 taagtttgtt gcaaatgtag actttgaact taaaaagaat actgattctg ctcttagtat
    12481 aaaagtgaga tattatactt atagtggtgg agcacatgga ttttatcaag atatagcata
    12541 taatgtggat atgagaactg gaaaattttt agaattaatg gacttattta aggataatac
    12601 taaatataaa gaagttatag atgaagaaat taaaagacaa atagctgaac ttgaaaaaaa
    12661 ggatgaagaa aatatcggaa tatataattt taaaggtata aaagagaatc aaaactttta
    12721 cttgcaagat gagaatctag ttgtatattt tgatttatat gatataactc catatgcagc
    12781 gggaatacca gaattttcaa taaataaagc gttgataagt tctatgctaa aaccagagta
    12841 tgtagattta tttgatttga aatagaagat taaattaaat gttatgctat aatatttagc
    12901 atagaaactt attaaaattg ggggtataaa aatgctactt ttaaaaaaag acgacataaa
    12961 aaaagtattt acaatgagag atgcaattga agctgataag gaagctttta gaatttattg
    13021 tgaaggaaag agtgtaaatc ctttgagaac caatatatct gttccaagtc aagatgctag
    13081 tatgttattt atgccaggat acgtagaaga attaggctgt gggggaataa aaattgtatc
    13141 tgtatttcct aaaaatgctc aaaaaggcaa accagtaatt ccagcaacag tactattatt
    13201 agatggagaa actggagaag ttagtgcagt attggatggt acttatgtaa ctcagattcg
    13261 tactggagca gcctctggag cagctataga tgtattggct aaaaaagatt ctaagattgg
    13321 agctttgata ggtctaggag gacaaggtga gccacaactt gaagccatga tagaagctag
    13381 aaatttggat gaggtaagag tttttagtag aaacagggaa agtagagaaa aatttgcaga
    13441 agaaatgaac ttaaaattaa gtaaatataa tactaaaata gttgcagttg aatcatctga
    13501 tgaagccata gataacgcag atgtgatagt tttagctact ccatcaaaac aaccagtact
    13561 taatggaaac ttagtgaaaa aaggagcttt aataagtgca gtaggttcat atatgccaga
    13621 tatgcaagag ttgtgcccaa actgtttaac tagagcgtct aagatatttt ttgaatcaac
    13681 agatgcagtt ttatctgagt caggagatat attaattcca ttaaaagaag gtaagataag
    13741 taaagatgat tttagtggtg atttaggaaa cgttataaat ggaactattc ctggaagaga
    13801 agatgatgat gagataattg tatttaaaac tgttgggata ggtgtacaag atgtcgtaac
    13861 agcaaaaaga atatacgata aagcaaaaga aaacggtatt ggaatagact ggaagtggta
    13921 aaatattcaa aataattcta tagttagaca aatttatgtt ataatgttga taaataaatt
    13981 ttttattatt ttaattgagg gaggatgttt gtatgaaaat ggtaaaaaag ttaatattgt
    14041 ctacgattat ggctctagtt ttagttctaa ctatagggtg ttctaatgca gaagaaaaag
    14101 taaagtcgaa ttttgaagct aatttagctg cattagaaat gtataagtat gatgaaaaag
    14161 gaatttctga tgcattaaca gaagctgaat ataaacagat tcaaacttat aacagcaagg
    14221 acaaggtttc aatgattgct caattgacac ctctttatgg taaagaaaat gctgaaaaga
    14281 tatacgaaac aattgttaaa ttaattcaag gtgttgaaaa gacagtaaca gtggaatctt
    14341 ctagtaagga taagataact ttgaaagttg taagtaaagc tttagatact agtaaagtaa
    14401 tgagtgaagc gaacacttta gtaaaatctt atgtaactga tgaaaagaat tacgataaaa
    14461 aagaaaaaga agttcaagat gatatgacaa aaatgattat agacctttac aataagaaac
    14521 caactaagga agttacaact atgattacat atactaaaaa agatggtaaa tggcaaccac
    14581 ctagtaatat acaaaagtta tgtgaaggaa tatatacaat ttaatttatt ttaaagacac
    14641 aaaactatct tttaagcata taataaaatg tttggaa
//
LOCUS       DAMJWP010000083        13827 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.087, whole genome shotgun sequence.
ACCESSION   DAMJWP010000083 DAMJWP010000000
VERSION     DAMJWP010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13827
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.087"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            98..328
                     /locus_tag="PC700_002036"
     CDS             98..328
                     /locus_tag="PC700_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581347.1"
                     /translation="MRLDMDSMLYVLIYMTLLVSIIGSIFYFCKDLLNHKIKYVLIRD
                     IFVNRITYSEERLPKQYIVFQKYDIWRYCSLF"
     gene            385..1035
                     /locus_tag="PC700_002037"
     CDS             385..1035
                     /locus_tag="PC700_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDF2581348.1"
                     /translation="MDFISSILQGLLSILFNFTGDFGISIVLITLIVKLVLMPLSLKQ
                     RFSVKKQQELAEKMEHIKEKYKNDTKELEKQIQVSSVESMKSMLGCLIVLVQMPVVYA
                     LYHVCLDMTKEFTTIIIPWIANLGTFDNLFILPCIYTLIMLASNLVNYIPYLKINSQV
                     KFNKQMAVVTTITSLILTVRTPVAIGLYFITSAIYSLIEDICFRMYFNRKNRIVTK"
     gene            1249..1920
                     /locus_tag="PC700_002038"
     CDS             1249..1920
                     /locus_tag="PC700_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2581349.1"
                     /translation="MAKILVVEDEKRMQSIIVEYMQKGGYTCITANDGVEALTILKSN
                     NIDLMILDIMMPYLDGFSVCRVSREMTNIPIIILTAKGEEEDKLKGYEYGADDYITKP
                     FSPKVLLAKVNALLRRYTTDIPKNSLSLGKIFIMVASRQVYVEDKLIDLTYKEFELLR
                     LFMENPNQVFSREKLLNCIWGYDFEGNTRTVDTHIKTLRKKLGSEGHHIVTLIRSGYK
                     FEVKE"
     gene            1917..3047
                     /locus_tag="PC700_002039"
     CDS             1917..3047
                     /locus_tag="PC700_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2581350.1"
                     /translation="MNSLRKIIEKLKNVDISNWIKQNFGLYSVRKKVFLLSKLSGALI
                     ILFYLVVEEFSINSKFEFWIWFIILVIFIMSIDFLLGKFISEPITSINKSAKSMSQLD
                     FSNPCTVNTNDEFGELSRSLNTMSTNLQQALSDLESANIQLEKDVNKERMLLEQRKEL
                     VDAISHEMKTPLGIIRAYTEGLMDEVDEEKKKNYMNVIIEETDRMNNMIVSLLDLSAL
                     EAGVSKLNPERFDFIEFVETVAGRLLIDIPDANFYFTYDLPEYEVFVVADKMRMEQVV
                     ENLIINAKKHVVYNGNLDLSITCENGLLLFEIYNDGRIIEQDEISKIWSKFYRSVQSQ
                     RKGGSGLGLAIVSQILTMQGLEYGVNNRDRGVEFYFMIPIDE"
     gene            3452..4297
                     /locus_tag="PC700_002040"
     CDS             3452..4297
                     /locus_tag="PC700_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2581351.1"
                     /translation="MIKVGELKFSYGKDKQILHGLNFEVKEGEIFGFLGPNGSGKSTT
                     QKILNGVLKGYNGHVSLFGKEVKAYTESLYQKIGVLFEFPYLYTNLSAIDNLEYFSSF
                     YPKNQRRDIYELLDLLEFKKEFINKPVSSYSKGMKQRISMARALVSNPKLLFLDEPTS
                     GLDPSGAVLFRKIIEEERKKGTTIFLTTHNMLDADLMCNNVAFIADGKIMVIDKPKNL
                     KMKNSNNKVEVEFVYNGNRDIEIVDIQELESGITFKYDEILSVHSKEPTLEEVFIKYT
                     GRMLV"
     gene            4297..5325
                     /locus_tag="PC700_002041"
     CDS             4297..5325
                     /locus_tag="PC700_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2581352.1"
                     /translation="MYSKFFTLVKKDFWMMIYGKFFVLTLGSLILYSCYINFGYVKFM
                     DEDSYHNNVYLYNPENISMSASPLINQVSSIGELKAKLDEDTSAVCIDMSSSKPKVIL
                     HESIKGVDNYRADYALSLLSSEKDHNSKISDNNAIIQINDKDTSEIKQRKDMTCEILF
                     FEIVAIGFLGIASLLFKEKQMGVMRITGILPVKRSLFILSKIFAFLICDLCFTVLLVV
                     FNIGFFEGVKILPQVLVQASILSIIMSLIGIGCSMLLRDFKQFSLIYLVVAVFITTPI
                     FLSANTSFEIDWIKYHPFYHVYSQLKNAFFMNFSKELVYFVVCSLTIIVLFLLVKYIF
                     NKELVREG"
     gene            5328..6341
                     /locus_tag="PC700_002042"
     CDS             5328..6341
                     /locus_tag="PC700_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2581353.1"
                     /translation="MIGLRYQLKSIKKDKMCIISFFLPVVMAILINFMGTIDISSLGE
                     IRFGIVKDTLPVQTEQWLKKYGMVSVYDTKMHLIREIKDIKTDTIGVVRDGKGIKTML
                     YGNEINAIKNVGKTLPEMFRLKGDLKDVEVTILPKDSILKEFQNVFIAMILITAMFMG
                     CTFNAMNIISEKEDGISFINEVLPQSRSQYIIQKVFLGLLCGCLSSMITVFICLDISI
                     LDMIMMLLLVVFSAFISSLIGLFIGKLSEGLMVGIVYIKIVMILFIGAPLLIYLLGVN
                     TSGIGNLCYIMPSVATFNGIMEIINGNVIIIKDIVILVVHCVVWFMIYIILDKKRKLS
                     FSR"
     gene            complement(6823..8073)
                     /gene="cme"
                     /locus_tag="PC700_002043"
     CDS             complement(6823..8073)
                     /gene="cme"
                     /locus_tag="PC700_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Cme"
                     /protein_id="HDF2581354.1"
                     /translation="MENKNTNSTWKKKVILFLASQYISLFGSSLVQMAMIWYVTLQTS
                     SGVWVTVLTVCSFIPQMIISFFAGVWADYYNRKVLIIVSDSTIAISTLVLILFMMSSN
                     GTTSELIAISVISVIRSLGSGVQTPAVNAMIPQLVPEEHLMRFNGINSSLQSIVQFIV
                     PIVAAAILGFGNKINQVLLIDVFTAIIAIVLLIFIKIPKHNIPKQTEKVSFFADMVSG
                     IKYCFSDKFIRKVLFTYGAFMLLCVPSGFLIGLLIERTFGKGYIYLSIIEMIGFLVMV
                     LGGLIIGVFGGFKNRNKTFFLAILSYAIFSIILGLVTQAWQFFIFMFFTNFSIPIIQA
                     SAMTMLQEHVAPQMLGRVLSLPIIIYTGFIPLGMMIFGPMADVISINNLIIVAGIILL
                     FFALAIPYSKQFYKQGISKSNESE"
     gene            complement(8159..8905)
                     /locus_tag="PC700_002044"
     CDS             complement(8159..8905)
                     /locus_tag="PC700_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2581355.1"
                     /translation="MDTYTTSEVADLIGVHPNTVRLYEELNLIPKPKRNTNGYRIFND
                     FHIEQFRFARTALKIEVVQRGLRKDALEIVRTSANRDFDKAISLVNQYLQQLEIEKSN
                     AEEALTIVAQILNGNTPETNKLFLTRKETAEHLNVTIDTLRNWEMNGLLKVKRKQNGY
                     RVYTDEDIKRLKIISALRCANYSLSAILRMLNALSSNPNTNIKTVLDTPKDDDEIISV
                     CDKLITSLKEAEHNAKTMKSHLQIMKKQFS"
     gene            complement(9020..10042)
                     /locus_tag="PC700_002045"
     CDS             complement(9020..10042)
                     /locus_tag="PC700_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HDF2581356.1"
                     /translation="MNKNNCLDLIFEKVDPSSVGMSSEKLSQLEPLIISQYSNINGII
                     IARKGKVVFEKYYNGYNADDTFHVASVTKSIISALIGIAIDKGYIKDVDQKVVDFFPE
                     YTCSPSEICKKAVTIRHLLTMTAPYPFKNMTESLERLCKQKDWIKYTLNQLGQKGEIG
                     TFKYSSAGTHLLSAIITKTTGKSAREFANEYLFKPIGMKVIPDYDMKDFGFEDFFGKN
                     LKGWAKDRQGYSIGGWGLTLSARDMLRFGFLYLNDGIWNNKRIISKSWIEESVKMNSN
                     EYGYLWWICKKDGVLWYSAMGTGGNAICCIPQYDLVIAIPSKIVFKPRDRWEFIEKYI
                     LTSIID"
     gene            complement(10042..12462)
                     /locus_tag="PC700_002046"
     CDS             complement(10042..12462)
                     /locus_tag="PC700_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF2581357.1"
                     /translation="MKEIKINGAKIHNLKNIDVSIPKDKLVVATGVSGSGKSSLMFDI
                     VFEEGRKQYLQSLGILAGIDSEDKFDNIQGLAPTIAVQQNIIRQSNPRSTVGTRTNIL
                     NMLALLYSVEGQIMCTMCDTPVSDNLICNNCGYEEERLRPSYFSYNSSDGMCMKCSGH
                     GAYFEINIEKLVLDKHDTLKQVLDRAKITPGYLRVFSKKFNDYLDIQFLQLPEEIRNE
                     VLYGHYENGKKSSSLSKVFHNRYKRGEDLNGVYTMTACSDCNGFKIGEEARRVLLNGK
                     HIGELGKMTILEIDDFLKILINQGNLNTFGTNLVNDILSKTRHLIESRLGHLSLYREM
                     STLSGGEMQRLFLNSHLDSEMDSLIYVLDEPTAGLHESEKIDILKSIKKLKDLGNTVI
                     VVEHDKNTIEMAEHIIDIGPKAGVEGGQLVYQGDLEGLLQSDKSLTGQYLSGKYPMPN
                     RTSNKNITHTDKIPCITIQNANTNNLKNVTVSLPLGAMVGISGKSGSGKSSLISDTLL
                     PLLRSQFNNHTINNQINSSDSEIGDEDDYAVIETIADRLIGTKHISGYSEISQSPIGK
                     NMNSTPASYIGIWDKIRTLFAGQPESLERGFTAGHFSFNSKGACPKCSGSGYEKVWLG
                     NNLSIDHICSECQGKRFNDESLSIKYKNKNIHDILNMSVSESVHFFKDIPNIVSHLNV
                     LEQIGMGYIKLGQPTPTLSGGESQRIKLAKEIGKKRNGNILYVLDEPTTGLSLYDTSK
                     LIQLLDELVENGNSVIVVEHDIDVLNVCDWIVELGPEGGDKGGYIIAEGSPKTLKENP
                     KSITGRYL"
     gene            complement(12548..13468)
                     /locus_tag="PC700_002047"
     CDS             complement(12548..13468)
                     /locus_tag="PC700_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2581358.1"
                     /translation="MTNAKSKKEYLNRICKVQDYIEEHLHEPLSLDELSNIAGFSKFH
                     FHRIFKAIEKETLAQYVNRLKLENATAFLIHRTDMTVTDIAHYFGFTDSAVFSRAFKN
                     YYKISPIKYRNNYSKNCKEPYKISQYNRSISKPKCKNTREVKGEIEILELDNMNTIYT
                     RYTGSYGNLTSTFPKLLERLFTYASEQNLLGLENTQILAIYHDNPQFTQEHQLRTSIC
                     MTIPDNIVVKENDNLGNMIIPSGKYLVGHFDISKSEYSDAWDFMYNEWLSNGNYKLRA
                     SFPFEVYLNNPHNHPQNRHFVDIYLPIEPF"
BASE COUNT         4858 a         1804 c         2190 g         4975 t
ORIGIN      
        1 ctataattat ttatatttaa aactaaatat aaatgttgga attatttagg atttgaaatt
       61 gtctaaagat aaatataagt gatgaagagg tgatgtgatg agattagata tggatagtat
      121 gttatatgta ctaatttata tgacattatt ggtatctatc attggaagta tattttattt
      181 ttgtaaagac ctcttaaatc ataaaattaa gtatgtttta ataagagaca tatttgtaaa
      241 tagaattaca tattctgaag aacggttgcc taaacagtat atagtttttc agaaatatga
      301 tatttggagg tattgtagtt tgttttaatt tgaaatgaat ctattataaa ttctaaagtt
      361 taaaaataaa aaggagagaa taaaatggat ttcatttcaa gtatattgca aggattatta
      421 agtatattat ttaattttac aggtgatttt ggaatttcta ttgtacttat aactttaata
      481 gttaaattag tacttatgcc actatcatta aaacaaagat tttctgtaaa aaaacagcaa
      541 gaattagctg aaaaaatgga acatataaaa gaaaagtata agaatgatac taaagaattg
      601 gaaaagcaaa tacaagtaag ttctgtagaa agtatgaaga gtatgctggg atgtcttata
      661 gttttggttc aaatgccagt agtatatgct ttatatcatg tatgtttaga tatgacaaag
      721 gaatttacaa caattataat tccatggatt gctaatttag gtacatttga taatttgttt
      781 atattacctt gtatatatac attaattatg ttagcttcaa atttagtgaa ttatatacca
      841 tatttaaaaa taaattctca agtaaagttt aacaaacaaa tggctgtggt gactaccata
      901 acaagtctta tactaactgt tagaactcca gtagccatag gtctgtactt tataacaagt
      961 gcaatttatt cattaattga agatatttgt tttagaatgt attttaatcg aaaaaataga
     1021 atagtgacaa aataatataa ttattattag aaatatttta gcctgctaac atagaaaaag
     1081 ttagtaggct atttttatct atgatatcaa ttccattaag aatagtctaa aaaatactat
     1141 caaaatgcta taaaaaatac tataaaaata ctataaaaaa atagttgaaa aataaataaa
     1201 ttaatataaa ctaaaataaa gcctacaaaa aagaggtgat aaatagaaat ggctaaaata
     1261 ctagttgtag aagatgaaaa aaggatgcaa agtattattg tagaatatat gcaaaaggga
     1321 ggatatactt gtataactgc taatgatgga gtcgaggcac ttacaatatt aaagagcaac
     1381 aatatagatt taatgatatt ggatattatg atgccgtatt tagatggatt ttcagtgtgt
     1441 agagtttcac gtgaaatgac taatatacct attatcatat tgacagctaa aggtgaagag
     1501 gaggacaaat taaagggtta tgaatatggc gcagatgatt atataacaaa accatttagc
     1561 ccaaaagtac ttttagcaaa agttaatgcc ttgttacgta gatacactac tgatattccc
     1621 aaaaattcat tgagtttagg aaagattttt attatggtag catcaaggca agtgtatgta
     1681 gaagataaat tgattgattt aacatataaa gaatttgaat tattgcgttt atttatggaa
     1741 aaccctaatc aagttttttc cagagagaag cttttaaatt gtatatgggg atatgatttt
     1801 gaaggaaaca cacgaactgt agatacacat ataaaaacct tacgcaaaaa gcttggtagt
     1861 gaaggacacc atatagttac attaattcgt tcaggatata agtttgaggt taaggaatga
     1921 atagtttaag aaagattata gaaaaactga aaaatgtaga tatttcaaat tggattaaac
     1981 aaaattttgg actatattca gttagaaaaa aagtgttttt actttctaaa ttatcaggag
     2041 cgttaattat attattttat ctggttgttg aagaattttc gataaactct aaatttgaat
     2101 tttggatttg gtttataata ctggttatat ttattatgag tattgatttt ttattgggaa
     2161 aatttatttc agaaccaatc acatctataa ataaatctgc aaaaagtatg tcccaacttg
     2221 atttttctaa tccatgtact gtaaatacta atgatgaatt tggagaactt tctaggagcc
     2281 tgaatacaat gtctacaaat ctacaacagg cattatcaga cctagaaagt gcaaatattc
     2341 aacttgaaaa ggatgtaaat aaggaaagaa tgctattgga acaaagaaag gagttggtag
     2401 atgcaatttc acatgaaatg aagactccac ttggcattat acgtgcgtat acagaggggc
     2461 ttatggatga agttgatgaa gaaaaaaaga aaaattatat gaatgttata atagaagaaa
     2521 ctgatagaat gaacaatatg attgtatcat tattagactt atctgcattg gaagcaggag
     2581 tttctaaact taacccagag cgttttgatt ttatagagtt tgttgaaact gtggcaggtc
     2641 gtcttttaat tgatatacca gatgccaatt tttattttac atatgatttg ccagagtatg
     2701 aagtttttgt agtggcagat aaaatgagaa tggaacaagt agtagagaat ctaattatta
     2761 atgcaaagaa gcatgttgta tataatggta atttagattt atcgattaca tgtgaaaatg
     2821 gcttgctttt atttgaaatt tataatgatg gtaggattat agagcaagat gagatttcaa
     2881 agatttggtc taagttttat cgtagtgttc aatctcagcg taaaggaggt tcaggtctag
     2941 ggcttgcaat tgtttcacaa atacttacaa tgcaagggtt agaatatgga gtgaataatc
     3001 gtgatagagg agtagagttt tattttatga ttccaattga tgaataattt agtattaatt
     3061 tataaattat aagtatctga tgtaaaatat ttttctttgt atacgtaagt atttaaagag
     3121 aataaaataa aaaacttaat gaaaaagtct aataaaaagt ttaatgaaaa gtcttgtttt
     3181 cctgatgaaa acaagatttt ttattaagct ttttattatt tcacttcgat ttcacaagag
     3241 agacactctg atttcaccaa atagaaataa aataggttaa ataaattaaa aaatagaagc
     3301 gtaattaaat aaggttacaa gggggagtac ttatgaaatg gtatacatgg ttgactatta
     3361 tattgataat attgctttct attccgctta aaattaaatt tataaaatgg tgggatagac
     3421 gacaaaaaaa tgataaagga ggttatgata gatgataaaa gtaggagagt taaaattttc
     3481 ttatggaaaa gataaacaga tattgcatgg acttaatttt gaagtaaaag aaggagaaat
     3541 ctttggattc cttggaccta atggttcagg aaaatccaca acacagaaaa tcttaaatgg
     3601 cgttttaaaa ggttacaatg ggcacgtatc cctttttggg aaggaagtta aagcatatac
     3661 agagtctcta tatcaaaaga ttggtgtttt atttgaattt ccttatttat ataccaattt
     3721 aagtgcaatt gataatttag aatatttttc gtctttttat cctaaaaatc aaaggagaga
     3781 catatatgaa ctcttagatt tgttagagtt taagaaagag tttataaata agcctgtatc
     3841 atcctactca aaaggtatga agcaacgtat tagcatggca agagcattgg taagtaatcc
     3901 aaagttgtta tttttagatg agcctactag tggacttgac ccatctggag ctgtactatt
     3961 tcgtaagatt attgaagaag aacgaaaaaa aggaacaact atctttttga ctactcataa
     4021 tatgctagat gcagatttaa tgtgtaacaa tgtggctttt attgcagatg gcaagattat
     4081 ggtaatagat aaacccaaaa atttaaaaat gaaaaatagc aataataaag ttgaagtgga
     4141 atttgtatat aatggaaatc gagatattga aattgtagat atacaagagt tagaatctgg
     4201 aataactttt aaatatgatg aaatcttaag tgtacattca aaagaaccta cactagaaga
     4261 agtttttatt aaatacacag gaaggatgtt agtgtaatgt attctaaatt ttttacctta
     4321 gtaaaaaaag atttttggat gatgatttat ggtaaatttt ttgtgctgac acttggctct
     4381 ctcatacttt attcgtgtta tattaatttt ggttatgtaa agtttatgga tgaagatagt
     4441 tatcacaata atgtatatct atataatcca gagaatataa gcatgagtgc atctccatta
     4501 attaatcaag tttcttctat aggtgaatta aaagcaaaac ttgatgaaga tacaagtgca
     4561 gtttgtattg atatgagttc cagtaagccg aaagtaattt tacatgaaag tattaaagga
     4621 gttgacaatt atagagctga ctatgcattg tcattattat catcagaaaa agaccataat
     4681 agcaaaatat cagacaacaa tgcaataatt caaattaatg ataaggatac atctgagata
     4741 aaacaaagaa aagacatgac ttgtgagatt ttattctttg aaattgtagc tattggattt
     4801 ttagggattg cctctttatt atttaaagaa aagcagatgg gagtaatgcg tattactggt
     4861 attttacctg taaaaagaag tctttttata ttatcaaaga tatttgcttt tttgatatgt
     4921 gacctatgtt ttacagttct tttagtagta tttaatattg gattttttga aggagtaaaa
     4981 atacttccac aagtattagt tcaagctagt attttatcaa ttattatgtc tttgattgga
     5041 ataggatgct caatgttatt gagagatttt aagcaatttt ctcttatata tcttgtggtt
     5101 gctgtattca taactacacc tatatttcta tctgcaaata catcttttga aatagattgg
     5161 attaaatatc atcccttcta tcatgtttat agtcagctta aaaatgcgtt tttcatgaat
     5221 ttttcaaaag aattagttta ttttgtagtg tgttctttga caataatagt tctattttta
     5281 cttgtaaaat atatttttaa taaagagttg gttagggagg gataatcttg ataggattac
     5341 gttaccagtt aaagagtata aaaaaagata aaatgtgtat aatttccttc tttttaccag
     5401 ttgttatggc gattttaata aattttatgg gaacaattga tatatcttca ttaggagaga
     5461 tacgttttgg tatagtgaag gatacacttc ctgtacaaac agagcagtgg ttgaagaagt
     5521 atggtatggt aagtgtatat gacacaaaaa tgcatttaat acgtgaaatt aaggatatta
     5581 aaacggacac aattggagtt gttagagatg gtaaaggaat aaaaactatg ctttatggaa
     5641 atgagattaa tgctataaaa aatgttggaa agacattgcc agagatgttt agactaaagg
     5701 gtgatttaaa agatgttgag gttacaatat tgcctaaaga tagtatttta aaagagtttc
     5761 agaatgtttt tattgctatg attttaatta ctgctatgtt tatgggatgt acctttaatg
     5821 caatgaatat tatatctgaa aaagaagatg gaatatcatt tataaatgaa gttttgccac
     5881 agagtagaag tcagtatatt atacaaaaag tatttttggg tcttttatgt gggtgtttat
     5941 cttcaatgat aactgtattt atatgtttgg atatttcaat attagatatg ataatgatgt
     6001 tgttactcgt tgtattttca gcatttattt catcacttat aggtttattt attggaaagt
     6061 tgtcagaagg tcttatggta ggaattgttt atattaaaat agtaatgatt ttatttatag
     6121 gtgctccttt gttaatatat cttctagggg ttaataccag cggtataggg aatttatgct
     6181 atataatgcc atcagttgct acatttaatg gtattatgga gattattaat ggtaatgtta
     6241 ttattataaa agatattgta attttggttg tacattgtgt agtatggttt atgatatata
     6301 ttattttaga taaaaaaaga aagttatctt ttagtagata actcagtata attataattg
     6361 ggacaattta ataggtgatt taatatactt tgtatagtgt aatttatgta gtgcaatttg
     6421 tatactttaa tttatgtagt gtaatagtta gttttatttt ataattattc aatataattt
     6481 agtaagaaat tcagtacaat tagagctact tagtggaaaa cttaataggt acttaaacta
     6541 tataaatact ttattttcct ccatttgtac tataaattta atttatgata taaatggagg
     6601 tattattata tgaggtatta ttatataaag ttaataaaga tagtatcaaa atattatttg
     6661 ctataaagag agggactcaa aataaagata aacctatttt tcaatgttga aatgataaaa
     6721 tatatttgat ttcatataaa aaattttctt cataaattaa aaagtccatt ttaagccccc
     6781 tcatttttaa ccagataaat cgagttaaac tatttaaata tattactcgg attcatttga
     6841 cttagatatt ccttgcttgt aaaattgttt tgaatagggg atagccaaag caaaaaataa
     6901 aagtataatc cctgctacga ttattaaatt attaatagaa attacatctg ccattggtcc
     6961 gaaaatcatc ataccaagag gaataaaacc agtatatata ataataggta agctaagaac
     7021 tcgacctagc atttgtggtg ctacatgctc ttgcaacata gtcatagctg atgcttgaat
     7081 aattggaatt gaaaaattcg taaaaaacat aaatataaaa aattgccaag cttgtgttac
     7141 aagacctaga ataattgaaa aaatagcata ggataatatt gctagaaaaa aggtcttatt
     7201 tctattttta aaaccaccaa aaactcctat aatcaatcca cctaaaacca taactaaaaa
     7261 tccaatcatt tcaataatag acaggtatat ataacccttg ccaaatgttc gttcaataag
     7321 aagtcctata agaaatcctg atggaacaca aagaagcatg aatgctccat aagtgaaaag
     7381 cacttttcta atgaacttat ctgaaaaaca gtactttatc cctgatacca tatctgcaaa
     7441 aaatgagact ttttcagttt gttttggtat attatgttta ggaattttga tgaatatcaa
     7501 taatacaata gcaataattg cagtaaatac atcaattaaa agaacttgat ttattttatt
     7561 accaaatcca agtattgcag cagctacaat aggcactata aattgtacta tagactgtag
     7621 actactattt ataccattaa aacgcattaa atgttcttct ggtacaagtt gaggaatcat
     7681 tgcgtttaca gcaggtgttt gtacaccact tcctagtgaa cgtataacag atattactga
     7741 tatggctatt aattcacttg ttgtgccatt tgatgacatc ataaatagaa ttagaactaa
     7801 tgtagatata gcaattgttg aatctgatac aattatgagt acctttcgat tgtagtagtc
     7861 tgcccataca ccagcaaaaa aagaaattat catttgaggt ataaaggagc atacagttaa
     7921 aactgttacc catacacctg atgatgtctg aagtgtgaca taccatatca ttgccatttg
     7981 tacaagtgat gagccaaata aggaaatata ttgacttgct aaaaaaagaa tgactttttt
     8041 cttccatgta gaattagtat ttttgttttc catcctattc ctcctttgat ttgtgtgttg
     8101 aatttttctt gctcttaagg ataacacaca aatcaaagtc tggagttaga gtggagggct
     8161 aggaaaattg ttttttcata atttgaagat gagatttcat ggtttttgca ttatgttcag
     8221 cttctttcaa tgaagttatc agtttgtcac acactgagat aatttcgtca tcatcttttg
     8281 gagtgtcaag gacagtttta atattagtat taggattaga agataaagca tttaacattc
     8341 gtaaaatagc tgaaagagaa tagtttgcac aacgaagtgc gcttataatt ttaagtcgtt
     8401 ttatatcttc atcagtatat acacgataac cattttgttt tctttttact tttaacaaac
     8461 cattcatttc ccaatttctt aaagtatcta ttgtaacatt taagtgttct gcagtttctt
     8521 ttctagttaa aaagagttta tttgtttctg gagtatttcc atttaaaatc tgtgcaacaa
     8581 tagtaagtgc ttcctcggca ttagattttt ctatttctaa ttgttgcaag tattgattta
     8641 caagactaat agccttatca aagtccctat tcgctgatgt tcttacaatc tcaagggcat
     8701 cttttcttaa tcccctttgt accacttcaa ttttcaaggc tgtacgagca aatctaaatt
     8761 gttctatatg aaaatcatta aagatacgat aaccattggt attacgctta ggtttaggta
     8821 ttaagtttag ttcttcatat aaacgtactg tattaggatg tacaccaatt aaatctgcta
     8881 cctctgatgt cgtgtaagtg tccattagta ttcaactcct tttagatata tagtattagt
     8941 cataataaca ccaaacaaaa gtctggtgtt atagtggagg gtttggttat ctaagagtaa
     9001 ttagtttatc taagagtgat taatctatta tagaagtaag tatatatttt tcaataaact
     9061 cccatctatc acgaggttta aacactattt tagatggtat ggctattact aagtcatatt
     9121 gtggaataca acaaatagca tttcctccag ttcccattgc tgaataccac aaaacaccat
     9181 cttttttaca tatccaccac agataaccgt attcattact attcatttta accgattctt
     9241 caatccacga tttagatata atacgtttat tattccaaat accatcattt aaatacagaa
     9301 aaccaaatcg caacatatct ctagcactca atgtaagtcc ccagccacct atagaataac
     9361 cctgtctatc ttttgcccaa cccttaagat tttttccaaa gaaatcttca aaaccaaaat
     9421 ctttcatatc ataatctggt ataactttca taccaatagg tttaaataag tactcattag
     9481 caaattcacg agcagatttt ccagtagttt ttgttataat agctgaaagt aggtgcgttc
     9541 cagctgaaga atatttgaaa gtaccaattt cgcctttttg accaagttga ttcaaggtat
     9601 attttatcca atctttttgt ttgcaaagtc tttctaaaga ctcagtcata tttttaaaag
     9661 ggtatggtgc agtcattgta agtaggtgac gtatagttac tgctttttta catatttcag
     9721 aaggactaca agtatattca gggaaaaaat caacaacctt ttggtcaaca tcttttatat
     9781 atcctttatc tattgcaata cctattaagg ctgatataat actttttgta actgatgcaa
     9841 catgaaaagt atcatcagca ttatatccat tatagtactt ttcaaagaca acttttcctt
     9901 ttcttgcaat aattattcca tttatattac tatattgtga gattattaaa ggttccagtt
     9961 gtgagagctt ttctgaactc atgccaacac ttgaagggtc tactttttca aatattaaat
    10021 ctaagcaatt gtttttattc attaaagata cctccctgtt atagattttg gattttcttt
    10081 tagagttttt ggagaacctt ctgcaattat ataacctcct ttgtcaccac cttctggacc
    10141 aagctcaaca atccagtcac aaacatttaa tacatctata tcatgttcaa caacaattac
    10201 agaatttcca ttttccacca attcatctaa taattgaatc aacttagaag tatcatatag
    10261 acttagacca gtagttggct catccaatac ataaaggata ttaccatttc gttttttacc
    10321 aatttctttt gcaagtttga ttctttggga ttcaccacca cttaaagtcg gtgtaggttg
    10381 gcctaatttt atatacccca tacctatttg ttctaataca tttaagtgtg aaacaatatt
    10441 tggaatatcc ttgaagaaat gtacagattc agaaacactc atatttaaaa tatcatgtat
    10501 atttttattt ttatatttaa tagataaaga ttcatcatta aatctttttc cctgacattc
    10561 actgcatata tggtctatac ttagattatt tcctaaccag accttttcat atccactacc
    10621 actacattta ggacaagcac ctttggagtt aaatgagaaa tgaccagctg taaatcctcg
    10681 ttctaacgat tctggctgac ctgcaaatag tgtacgtatc ttatcccata taccaatata
    10741 agaagcagga gttgagttca tatttttgcc aataggagat tgagatatct ctgaatatcc
    10801 agaaatatgt ttagtaccaa tcagtctatc tgcaatagtt tcaataactg cataatcgtc
    10861 ttcatcacct atttcgctat ctgaagaatt tatttgatta ttgatagtat ggttattaaa
    10921 ttggcttcta agaagaggaa gtagggtgtc tgatataagt gaactcttgc cactacctga
    10981 ttttccagat attccaacca tagcacccaa aggtaatgat acagttacat ttttaagatt
    11041 atttgtattt gcattttgaa tagttataca aggtatttta tcagtatgtg taatattttt
    11101 attgcttgtt ctattaggca ttggatattt tccagataga tactgcccag taagtgattt
    11161 gtcggattga agtagacctt ctaaatctcc ttgatatact aattgaccac cttcaacacc
    11221 agcttttgga ccaatatcaa taatatgttc tgccatttca attgtgtttt tatcgtgctc
    11281 aactactatg accgtattgc ctaaatcttt aagtttcttt attgatttta agatgtctat
    11341 tttttctgat tcatgcaatc cagctgttgg ttcatcaagc acataaatta atgagtccat
    11401 ctctgaatcg agatgtgaat ttaaaaatag tctttgcatt tccccaccac tcaaagttga
    11461 catttctcgg tataaggata gatgacctaa tcgtgattct attaaatgtc gagttttgga
    11521 taaaatatca tttacaagat ttgtaccaaa tgtgtttaaa ttaccttggt ttattaagat
    11581 tttaaggaaa tcatcaattt ctaaaatcgt catttttcca agttctccaa tatgttttcc
    11641 gtttaaaagt actctacgtg cttcttcacc aattttaaaa ccattgcaat ctgaacaggc
    11701 agtcatagta taaactccat ttaaatcctc accccttttg taacgattgt gaaatacctt
    11761 tgataaagag gaactttttt taccattttc ataatgacca tagagtactt catttcttat
    11821 ttcttcaggt agttgtaaga attgtatgtc taaataatcg ttgaattttt tagaaaatac
    11881 tctcaaatat cctggtgtaa tctttgctct atctaagact tgttttaggg tatcatgctt
    11941 atcaaggact aatttttcaa tattaatctc aaaatatgca ccatgtccag agcacttcat
    12001 acacattcca tctgaagagt tgtatgagaa ataacttggt cttaatcgtt cttcctcata
    12061 cccacaatta ttgcaaatta gattatcgct tacaggagta tcacacatag tacacattat
    12121 ttgaccttct actgaataca atagtgcaag catgttgaga atattggttc ttgtgcctac
    12181 tgttgaacga ggattgctct ggcgaataat attttgttga acagctatag tgggagctaa
    12241 accttgaata ttatcaaact tatcctcact atcaatacct gcaagtatac ctaaagattg
    12301 cagatattgt ttacgacctt cctcaaatac aatgtcaaac ataaggctag acttacctga
    12361 gccactgaca cctgttgcaa caacaagctt atctttgggg atagaaacat ctatattttt
    12421 aagattgtgt atctttgctc cattaatttt tatttctttc attggttcac ctcattttat
    12481 caattttatc aaaccgtaaa atagggatat attaatcctt aatacaagaa tagattaggt
    12541 gaggttatta aaaaggttct ataggtaaat atatatcgac aaagtgtcta ttttgagggt
    12601 gattgtgtgg gttgttaaga tatacttcaa aagggaagga ggcacgtagc ttataattac
    12661 catttgatag ccattcatta tacataaaat cccacgcatc actatattca gattttgaaa
    12721 tatcaaaatg tcccacaaga tattttccag aagggattat catattgcca agattgtcat
    12781 tctctttgac aacaatgtta tctggaatgg tcatgcaaat actagtcctt aattgatgct
    12841 cctgtgtaaa ttgtggatta tcatgataaa tggctaatat ttgtgtgttt tcaagtccaa
    12901 gtagattctg ctcacttgca tatgtgaata accgttctaa aagttttggg aaagtactgg
    12961 ttagattacc atatgaacca gtatatctag tgtaaatagt attcatatta tcaagttcta
    13021 gaatttcaat ttcaccttta acctctctag tattcttaca tttaggtttt gatatgcttc
    13081 tattgtactg agaaatttta tatggttctt tgcaattctt gctataattg tttctatatt
    13141 ttatagggct tattttatag taatttttaa atgctctcga gaaaactgct gaatcagtaa
    13201 agccgaagta gtgtgcaata tcagtaacag tcatgtcagt tcgatgaata agaaaagcag
    13261 ttgcattttc tagttttagc ctatttacat attgtgccaa agtttctttt tctattgctt
    13321 tgaagattct atgaaaatga aattttgaaa aacctgctat attagaaagt tcatcaagag
    13381 aaagaggttc atgtaaatgt tcttctatat agtcttgcac tttgcaaata cgatttagat
    13441 attctttttt acttttagca tttgtcatat atagccctcc ttattgtgta aaatgtgtat
    13501 taattaattt atagcaatat agtaataaaa ttttatgttt ttgcaattta tgataaataa
    13561 gatagcgttt aattttttat aattgataaa agatatttat aattaataaa agatataaga
    13621 tgccttatat ttatttttgc atataagtat agtcttcgta ataattagta taccatagtg
    13681 atttattttt aattgtgtat ttatataaat acacaactta tgatatggac gaattattat
    13741 gcaaataagc tgtaattaaa taaattgcaa tataaaccag ttgaaacata agttagggaa
    13801 atattatcaa agttattatt tagcata
//
LOCUS       DAMJWP010000084        13479 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.088, whole genome shotgun sequence.
ACCESSION   DAMJWP010000084 DAMJWP010000000
VERSION     DAMJWP010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13479
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.088"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            305..1138
                     /locus_tag="PC700_002048"
     CDS             305..1138
                     /locus_tag="PC700_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HDF2581359.1"
                     /translation="MRNKKKKINRKKLYLLLSAIVVCLAFIVVGVRVSSLNMKENEIS
                     RNAKLSSSTITDIVSNTSIEVSKGPSKSSGKIAYITIDDGPSKFTDEMIKTLNKYNVK
                     ATFFMIDGNMKEYPQQVKNIIKNGNTAGFHSVSHDIHKLYVTSTSAKEEFDTNDQTFY
                     KITGKHSKVIRIPYGSKPYTPQASYQALVDAGYKIWDWDLDTEDWRSNSSQIVQNVKN
                     HIKNRKGEDKDQLVVLMHEKKQSAEALDSVLKFLSDEGYEFAPVDQNQIPKNYWLRNL
                     E"
     gene            1231..2163
                     /locus_tag="PC700_002049"
     CDS             1231..2163
                     /locus_tag="PC700_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2581360.1"
                     /translation="MVGILSLMMVGCNKSLAKVNDVEITKEQYKKTKAVLSATNNYIN
                     GQSLDELEKTLDKKGRNKLENVIISFMVDNELLYQEAKDKGLTPSKSEVDSKYQELED
                     KMNLNTSYKEKMDKAGVDKEYLKQEISRDLAIDKNKKAFEDRINISDNDMEAYYTSHK
                     KDFNVEEVSASQILISTLDKNKKEVSKDKKEALKKKADNILTKIKNGESFESLAKKYS
                     DDKATGKNGGQLGYFTKDDKNAEFTKEVFKLKKNEVSNVFETSYGYHIVKVTDKRERQ
                     KSFNECQSLIRESILNEKYIEHIKKLNEDAKIDR"
     gene            2215..2697
                     /locus_tag="PC700_002050"
     CDS             2215..2697
                     /locus_tag="PC700_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2581361.1"
                     /translation="MFKSLKRTKVEKYIIDNKDSFYRIAYSYTKNEEDALDVVQEAMY
                     KALYSVENIKEVNYIKTWFYKILVRTSIDFIRKNRKYNNMTDIDLIDETGEYDKYTDL
                     DLRRALEELPIEYKSIIILRFFEDLKIEEVAIILDENVNTVKTRLYTALKKLKLKIEE
                     "
     gene            2709..3578
                     /locus_tag="PC700_002051"
     CDS             2709..3578
                     /locus_tag="PC700_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDF2581362.1"
                     /translation="MTSKERLNKLKDEYHKIPIPKKLDTIINHEKIENIYREKNKKVN
                     RLRFKVAIAFACIFTVLVNISPVFADNFSKIPVIGVIVEVITIKNYSLKSENYEAEID
                     IPKIRGLKDKNLEQRLNSSFMEDGKRLYHQFQERMEKIQSSKNKGYKSLSLSYSVKNN
                     SKKFLSIEMTKNEIEASSYVSKAHYTIDKKRQIVLTLPMLFKDDKYIKVISDNIKEQM
                     REQMKKDSTKSYFIDQKKDLPVEDFKTINKYQDFYFNKNEDLVICFDEYEVAPGYMGA
                     VEFVIPYKVIKDL"
     gene            3621..4376
                     /locus_tag="PC700_002052"
     CDS             3621..4376
                     /locus_tag="PC700_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDF2581363.1"
                     /translation="MTTYKKLKKSIFKISIFSICLLVSLVDINPIFAGSSSNETSNSD
                     TIKVLNAKSLNFKQGNYEVNVSIPKIKGLKDKTLENKLNNEFLQDGKKIYSEFQNKMK
                     KENNKGHRYLNVIHNVKNNSKDFLSIEMTKEEIEDSSSITKKHYTVDKNRGILLNLPI
                     FFKDSRYIRAISDNIKEQMRNQIKKDDTKSYFIDQDKDFPVKDFESINKYQDFYFNKN
                     GNLVISFEEYEVVPGYMGAVEFVIPNKVIKRLR"
     gene            4418..5239
                     /locus_tag="PC700_002053"
     CDS             4418..5239
                     /locus_tag="PC700_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HDF2581364.1"
                     /translation="MNIYEQAIRLIENNEDFAFATITSHSGSTPRETGAMMIVKNDST
                     IFGSVGGGSVEAECIKHAINVIKNRESMLYKFTLNKSDVAKLGMICGGTGEIQIDFID
                     SKLKSNIEKFNKRLKENTSKAYIFGAGHISRDVAVILSLLEFRTVVIDDREEFANYER
                     FPESEVVVLDSFENIPDFPTDENSYIIILTRGHLYDSSALEWALKRETGYIGMIGSRT
                     KIGLTYEKLMKKGFKKEELSKVHAPIGIKLNAQTPAEIAVCIAAELINCRANKEK"
     gene            5244..6005
                     /locus_tag="PC700_002054"
     CDS             5244..6005
                     /locus_tag="PC700_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HDF2581365.1"
                     /translation="MNTGSKDIDTLNVNFRGIEIKAVLVYKNRKNISIKIDPFGKIIV
                     MSPPKISKKIIKDIIIEKGDWILKKSEKYKGREEVYKQRMFITGEKFLYLGEEYHLVI
                     KELLKDSVKKSNCRITINEYQIVVQTNDTSTDFIKKSLKSWYKMESERTVLERIDFLK
                     LNCEIMKQLVPASVKVKEQNKRWGSCTAQKNIYINSKISMARLDVIDYVIIHEFSHLV
                     HMNHSKKFYDLVKSIMPDYKDKENWLKINGYKIII"
     misc_binding    6166..6423
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6536..6775
                     /locus_tag="PC700_002055"
     CDS             6536..6775
                     /locus_tag="PC700_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDF2581366.1"
                     /translation="MENTVYAKLNQGIDSFLRVAMTLRRREVDIQSISMTVDNMNNSG
                     IKLIVNEEKTSLDSVLNHMKKLHDIREITVERMRH"
     gene            7045..8040
                     /gene="ilvC"
                     /locus_tag="PC700_002056"
     CDS             7045..8040
                     /gene="ilvC"
                     /locus_tag="PC700_002056"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HDF2581367.1"
                     /translation="MKMARMYYEKDVDLEVLKNKKVAVLGYGSQGHAHAQNLRDNGVH
                     VVIGLYDGSKSAQKAKEDGFEVKSVAEATKESDLTMMLMPDEKQKKVYEESVKDNLKE
                     GQTLAFAHGFNIHYNQVQPPEFVDVVMVAPKGPGHLVRNVFTKGSGVPALFAVYQDYT
                     KKATETVLAYAKGIGATRAGVLETTFKEETETDLFGEQSVLCGGISELIKLGYKTLVD
                     AGYQKEVAYFECLHEMKLIVDLIYEGGFERMRYSISDTAEYGDYVSGKRVITDAAKQG
                     MQNVLEDIQNGKFAKAWIKENEEGRENFLKTREEEYNTEIAEVGRNLRSMMSFLK"
     gene            8093..9781
                     /gene="ilvB"
                     /locus_tag="PC700_002057"
     CDS             8093..9781
                     /gene="ilvB"
                     /locus_tag="PC700_002057"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HDF2581368.1"
                     /translation="MNGAKVILECLKKEGIDTIFGYPGGAVIPLYDALYDYSDDFKHI
                     RTSHEQGLVHAADGYARSTNTVGVCFTTSGPGATNAITGIATAFMDSSPMVVISGQVP
                     TSLLGKDSFQEIDITGATLSMTKHNYLVRNTKELVPTIKEAFRVANSGRKGPVLVDVP
                     KDLFLAEMDFSGEDYDLCQIDDYMDYKSDFDLDDETNIKLLNEAIDIIKESKKPVIYA
                     GGGVKSSDSEEILEKFATKIDTPVLNTLMGLGNIDRKNELSLGMVGMHGSRESNLALS
                     NSDLVIAIGARFSDRVISKSSEFAKNAKIIHIDIDPSEISKNIESNVSLVGDVKLVLS
                     LLIERVESKNNSNWKEEIKTFRKSEGVQTYEFHPQNILKKINEKYETLKKPTVVVTDV
                     GQHQMWSAKYWNFKGNKSFITSAGLGTMGFGLGAAIGTKAGNVDKNVVLVTGDGSFRM
                     NCNELATVANYNVPMLILLLNNRTLGMVRQWQKLFSNQRYSQTDINENVDYVKLVNAY
                     NIDGYKVSNMEELGKVLDVIDFNKPVFLQCDIDKDYDVYPIVAPNDALENLICN"
     gene            complement(10139..11269)
                     /locus_tag="PC700_002058"
     CDS             complement(10139..11269)
                     /locus_tag="PC700_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDF2581369.1"
                     /translation="MKGGVRKRSNKWYYYFDLGIVEGKRKKVERVGGNTKKEAEKALR
                     EALNEYENSGIVFEESNISLSDYLDFWYKEYVLLNCKYNTQESYRINIEKHIKPKLGA
                     YKVKALTPAILQNFINKKYKEDYSQNTLQVLKAILHRSLKSAVHPYKHIRENPMQYVS
                     IPKTKSKTETNKVKTITLEEFNQILNIFPQDSFQRIVLLIGFHTGMRRGEIIALKWDN
                     IDLDNKTITVKHTLIKKPNGMFELGQPKTESSCRTIFTGDTLIKALKEHKLYQKKMKL
                     KYGEFYFDSDWVCTKENGQQVNTHTLDTIVRQIRVALNNDFHFHSLRHAHATLLLENG
                     ANIKDIQNRLGHSQLSTTMDTYSHVTDKMKNETVDIFEKITN"
     gene            complement(11348..12055)
                     /locus_tag="PC700_002059"
     CDS             complement(11348..12055)
                     /locus_tag="PC700_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HDF2581370.1"
                     /translation="MSIKNGKDFLYVIWKDPKSRRQFIIGKLAKNGLYEFSYEYEIDE
                     AIKAGFNALISFDDISKTYYSEDLFSPFASRLPDKKRKDINKILDKYDMSEYDTYELL
                     KRSGARLPIDTLEFVDPIPLDIDKNIIRYFYIAGVRHYAGCEGHECDNSIELESNEAL
                     VLELEPENKFDPHAIKVFDKKENHIGYIPRYYSGELTSIINRGLKYNCIIKFVNKENN
                     CDECIKVKLEILFDEYN"
     gene            complement(12068..12937)
                     /locus_tag="PC700_002060"
     CDS             complement(12068..12937)
                     /locus_tag="PC700_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HDF2581371.1"
                     /translation="MIKNFDEWKEYDGFSYGSGRSEKIWLINEKTKDIGLFKFPKTKE
                     TGEYWAEKLACEIASLLKIPCANVEIGTYNKRIGSMSYLINKNDEKLIEGVWLINKYY
                     PCYNTDKLYDYKNNEYYSLKMIFKSIEEYNIKNDFLKMLIFDYLIGNRDRHQNNWALL
                     ESQNKITLSPLYDNGSSLCCYVNIENIDSFFTDHQRFEALINTRSRSRIRIDGKNKKE
                     PTHLEVLKYLNSNYYEQTIDFVEKIINLMNEVNINTLIDNYSENIISPKLKKLLKIYL
                     KEKVLRIKNIYNL"
BASE COUNT         5306 a         1475 c         2158 g         4540 t
ORIGIN      
        1 ttttggtatt tattatttgt ttaatatttc taatttataa aaaaaaaatt ataaatttta
       61 aaaattataa atgacatttt aactagaatt aattgtaaaa aacactatat aatactcaat
      121 tttcaacatt ctatacaatt atacagaaat tatacagcat aataatcacc gaaataacaa
      181 attttaaaaa ttaagcaaaa ctttttgtcg aagttcaacg tctatatata tgtagtgtaa
      241 atttttggga taaaatacta caaaaatata aatgtaaaaa tatagtaata ttggaggtcg
      301 aacgatgaga aataaaaaga aaaaaataaa tagaaagaag ttgtatttgc ttttaagtgc
      361 tattgtagtg tgtctggctt ttatagtagt tggtgttcgc gtcagctctt taaatatgaa
      421 ggaaaatgaa attagtagaa atgcaaaact gtcaagttct actattacag atatagtatc
      481 taatacatca atagaagtta gtaaagggcc aagtaaaagt tctggaaaaa tagcatacat
      541 aactatagat gatggcccat ctaaatttac tgacgagatg ataaaaactt taaataaata
      601 caatgttaaa gcaacttttt ttatgataga tgggaatatg aaggaatatc cacagcaagt
      661 taaaaatata attaaaaatg ggaacacagc aggttttcat agtgtatcac atgatattca
      721 caagctttat gttactagta catcagcaaa agaggaattt gatacaaatg atcaaacttt
      781 ttataagata actggtaaac attcaaaagt aataagaata ccttatggaa gcaaaccata
      841 tacaccacaa gcctcttatc aggctttagt tgatgctgga tataaaattt gggactggga
      901 cttagataca gaagattgga gatctaattc ttctcaaatt gtacaaaatg taaaaaatca
      961 tattaagaat agaaaaggtg aagataaaga ccaacttgta gttttaatgc atgagaaaaa
     1021 acagagtgca gaagctttag attcagtttt aaaattctta tcagatgaag gttatgaatt
     1081 tgcgccagta gatcaaaacc aaataccaaa aaattattgg ttacgtaatt tagagtagtt
     1141 aaaatataag aatattgatt gatattttta gaaattgaga ataaaaatga gtgaggtgta
     1201 ggtatattga ataaaaaact ttatatagga atggtcggaa tattgtcttt gatgatggtt
     1261 ggttgtaata aaagtttagc aaaagtaaat gatgtggaaa tcacaaaaga acagtacaaa
     1321 aaaacaaaag cagttttatc tgctactaat aattatataa atgggcagtc tttagatgaa
     1381 ttagaaaaaa ctttggataa aaaaggtaga aataaattag aaaatgttat aatatcgttt
     1441 atggtggata atgaactttt atatcaagag gctaaggata aaggactaac tccaagtaaa
     1501 agtgaagtag actcaaaata tcaagaacta gaagataaga tgaatttgaa tacaagctat
     1561 aaggaaaaaa tggacaaagc aggtgttgat aaagaatatt taaaacaaga aatatctaga
     1621 gatttagcaa tagataaaaa taaaaaggcc tttgaagata gaataaacat aagtgataat
     1681 gatatggaag cctactatac aagtcataaa aaagatttta atgtagaaga agtaagtgct
     1741 tctcagatac ttatatcgac tttagataaa aataaaaaag aagtaagcaa agataaaaaa
     1801 gaagctttga agaagaaggc agataatata ttgactaaaa tcaaaaatgg agaaagtttt
     1861 gaaagtttag ctaaaaaata ttcggatgat aaagcaactg gaaaaaatgg aggacagcta
     1921 ggatatttta caaaggatga taaaaatgct gagtttacaa aggaagtgtt caaattaaag
     1981 aaaaatgaag tttcaaatgt gtttgaaact agttatggat atcatatagt caaagttact
     2041 gataaaagag aaagacaaaa aagttttaat gagtgtcaga gtttaataag agaatctatt
     2101 ttaaatgaaa agtatattga gcatataaaa aaactgaatg aagatgctaa aatagacaga
     2161 tagaatttct aacagctcaa tcctagcctg taatcggagg gagattttac aggaatgttt
     2221 aagagtttaa agagaacgaa agttgagaaa tacattatcg ataataaaga ctctttttat
     2281 agaatagcat atagttatac gaagaatgaa gaagatgcct tagatgtggt tcaagaagcc
     2341 atgtataagg cactatactc agtggaaaat attaaagaag taaattatat aaagacatgg
     2401 ttttataaaa ttttagttag aacgagtata gattttatta gaaaaaaccg taaatataat
     2461 aatatgacag atattgacct tatagatgag actggagagt atgataaata tactgatttg
     2521 gatttaagaa gagctttgga agaattacct attgaataca aatctattat aatattaaga
     2581 ttttttgaag atttaaagat tgaagaagta gcaataatat tggatgaaaa tgtgaatact
     2641 gttaaaacta ggttatatac tgctttaaaa aaactaaaat taaaaattga agagtaggga
     2701 taaaaagaat gacaagcaaa gaaaggttaa ataagttaaa agatgagtat cataaaattc
     2761 caatacccaa gaaattagat actataataa atcatgaaaa aatagaaaac atatatcgag
     2821 agaaaaataa aaaagtaaat agattaagat ttaaagttgc aatagccttt gcatgtatat
     2881 ttacagtatt agtaaatata agtcctgtat ttgcagacaa tttttctaag atacctgtaa
     2941 ttggagtaat agtagaagta ataactataa aaaattatag tttaaaaagt gaaaattatg
     3001 aagctgagat tgatattcct aaaatacgag gtttaaaaga taaaaactta gaacaaagat
     3061 taaacagtag ttttatggaa gatggaaaaa ggctttatca tcaattccaa gagagaatgg
     3121 agaagataca atcatcaaag aataaaggat ataaatcttt aagtttgagt tatagtgtaa
     3181 aaaataattc taaaaaattt ctatcaatag agatgacaaa aaatgagata gaggcttcat
     3241 catatgtgag taaggcacat tatactattg ataaaaaaag acaaatagtt ttgactttgc
     3301 caatgctttt taaagatgat aagtatataa aagttataag tgataatata aaagaacaaa
     3361 tgagagaaca aatgaaaaag gatagtacaa aatcctattt tatagatcaa aaaaaagact
     3421 tgcctgttga agattttaaa acaattaata agtatcagga tttttatttt aataaaaatg
     3481 aagaccttgt aatttgtttt gatgaatatg aagtagcacc tggttatatg ggtgcagttg
     3541 agtttgtaat accatataaa gtaataaaag atttgtaaat tgaatagtag tttatatata
     3601 catattttaa gaaggacaat atgactacat ataaaaagct taaaaaatct atatttaaaa
     3661 tttctatatt ttcaatatgc ttattagtct cattagtaga tataaaccct atatttgcag
     3721 gtagttcctc taatgagact tcaaatagtg atacgataaa ggtgttaaat gctaagagtc
     3781 tcaactttaa acaaggcaat tatgaagtga atgtgagtat tcctaaaata aaaggtttaa
     3841 aagataaaac tttagagaat aaattaaaca atgagttttt gcaagatggg aaaaaaattt
     3901 acagtgaatt tcaaaataaa atgaaaaagg aaaataataa gggtcataga tacctaaatg
     3961 taatccataa tgtcaaaaat aattctaagg actttttatc aatagaaatg acaaaagaag
     4021 agatagaaga ttcctcgtct ataactaaga agcattacac tgttgataaa aatagaggaa
     4081 tactattaaa tttaccaata ttttttaaag atagtagata tattagagct ataagtgata
     4141 atataaaaga gcagatgaga aatcaaatta agaaagatga tacaaaatct tattttatag
     4201 accaagataa agacttccca gtgaaagatt ttgaatctat aaataagtat caagattttt
     4261 attttaataa aaatggaaat cttgtgatta gttttgaaga atatgaagta gtacctggtt
     4321 atatgggagc agtagaattt gtgataccaa ataaagtaat aaaaagacta agatagatat
     4381 atagaagatt tttagataga ctggtaagga gaattatatg aatatatatg aacaagcaat
     4441 aagattaata gaaaataatg aagatttcgc atttgctaca ataacgtctc attcaggttc
     4501 aacaccacgt gaaactggag caatgatgat tgtgaaaaat gattctacta tatttggttc
     4561 agtaggagga ggtagtgtag aagcagaatg tataaaacat gcaattaatg ttataaaaaa
     4621 tagagaatca atgttgtata aatttactct aaacaaatct gatgtggcaa aactaggaat
     4681 gatatgtgga ggtactggag aaatacaaat agattttata gatagtaaat taaaaagtaa
     4741 tatagaaaaa ttcaataaga gactaaagga aaatacaagt aaagcatata tatttggtgc
     4801 aggtcatatt tcaagagatg ttgctgtgat actatctctt ttagaattta gaacagtagt
     4861 aatagatgat agagaagaat ttgctaatta tgaaaggttc ccagaatcag aagtagtagt
     4921 tttagattct tttgaaaata taccagattt tccaactgac gaaaatagct atataattat
     4981 tttaacaaga ggtcatctat atgattcaag tgctttagaa tgggctctta aaagagaaac
     5041 tggttatata ggtatgattg gaagtagaac aaaaattgga ttgacgtacg aaaaactcat
     5101 gaaaaaaggt tttaagaagg aagaattatc aaaagtacat gcaccaatag gcataaaatt
     5161 gaatgctcaa acaccagctg aaatagctgt atgtatagct gctgaactta taaattgtag
     5221 agctaataag gaaaaataaa ctaatgaaca cagggagtaa agatattgat actttaaatg
     5281 ttaattttag agggattgaa ataaaagcag ttttagtata taaaaataga aaaaatattt
     5341 ctataaaaat agatccattt ggcaaaataa ttgtaatgag tcctcctaaa atatccaaaa
     5401 aaataataaa agatataatt attgaaaaag gagactggat acttaaaaag tcagaaaaat
     5461 ataaaggaag agaagaagta tacaaacaaa gaatgtttat aactggcgaa aaatttcttt
     5521 atttaggtga agagtaccat ctagttataa aagaactgtt gaaagattct gtcaaaaaaa
     5581 gtaactgtag aataactatt aacgaatatc aaatagttgt tcagacaaat gatacaagta
     5641 cagattttat taaaaaatct ttaaaatctt ggtataagat ggaaagtgaa agaacagtat
     5701 tagagagaat agattttctt aaattaaatt gtgaaataat gaagcaatta gttccagcaa
     5761 gtgttaaggt aaaagaacaa aataagagat ggggaagttg tacagctcaa aaaaatattt
     5821 atataaactc aaaaatatct atggcaagat tagatgtaat tgattatgta ataatacatg
     5881 agtttagcca tttagtacat atgaatcact ctaaaaaatt ctatgattta gtaaaatcca
     5941 ttatgcccga ttataaagat aaagaaaatt ggctaaaaat aaatggatat aaaataataa
     6001 tctgaaaacg attattccaa aatacaggcg taaagtataa aagaagtatt attgtgaaaa
     6061 aaataaataa tctgaaaatt ttaaaaaaag tcattgacat tgtaaatgat agggaatata
     6121 atctatatta acaacaaaac agaattaaaa tataaaatta aaaaattaaa agcaatgaca
     6181 gagacaacaa tactaatagt gttatccaca gagaaccaat gttgctgaga attggtgtta
     6241 acagtagtat aatgatcact ctggagcttt tgtactgaat atttttagta agtacaaacg
     6301 tatgtcaacg ttacatgact aaggttaagg taattaatta cccgacctga aaaaagaggg
     6361 gtagatgtat tttttatatc tacaaataag gtggtaccgc ggaatataac tttcgtcctt
     6421 ataagtaata tttatattgc ttataaagat gaaagttttt ttattttaag cgatataaat
     6481 aaatttaatc catataaaat taataaaaaa ataataaaaa taaggaggga tttttatgga
     6541 aaatactgta tatgctaaat taaaccaagg aatagattct tttttaagag tggctatgac
     6601 tctaagaaga agagaggttg atatacaatc aataagtatg actgtagata atatgaataa
     6661 ttcaggtata aaattaatag taaatgaaga aaagacatct ttagattctg tcttaaacca
     6721 tatgaaaaaa ctacatgata taagagagat aactgttgaa agaatgagac attaattaaa
     6781 tatattttat aaagctaaag ataaatatta aataagaaaa atcttagatt aaaatataag
     6841 tgtcattgaa tttatcagtt tatcacagtt aataaattta ttagtttgtt gtagctaata
     6901 aaaactgaaa attctaaaat tataattgta agattatata aatttagaat ttaaatctgt
     6961 tgaataatat taaaacaatg ataatgagta tgaatttcta ataaaaataa atatcataaa
     7021 aataagaaaa agtattggga ggaaatgaaa atggcaagaa tgtattatga aaaagatgtg
     7081 gatttagagg ttttaaaaaa taaaaaggtt gcagttttag gttatggaag tcaaggacat
     7141 gcacatgcac aaaatcttag agataacggt gtacatgtag tgataggtct ttatgacgga
     7201 agtaaatcag cacaaaaagc aaaagaagat ggatttgaag ttaagagtgt agcagaagct
     7261 acaaaagaaa gtgatttgac aatgatgtta atgcctgatg aaaaacaaaa gaaagtttat
     7321 gaagagagtg taaaggataa cttaaaagaa ggtcagacat tagcttttgc tcatggattc
     7381 aatatacatt ataatcaagt acaaccacca gaatttgttg atgttgtaat ggtagctcct
     7441 aaaggacctg gtcacttagt tagaaatgta tttacaaagg gaagtggagt tcctgcacta
     7501 tttgcagtat atcaagacta cactaaaaaa gctacagaga cagttttagc atatgcaaaa
     7561 gggataggag caacaagagc aggtgtttta gaaactactt ttaaagaaga aacagaaaca
     7621 gatttatttg gagaacaatc agtactttgt ggtggaataa gtgaacttat aaaattggga
     7681 tataaaacgc ttgtagatgc aggttatcaa aaagaagtag catactttga atgtttacat
     7741 gaaatgaaac taatagtcga ccttatatat gaaggtggat ttgaaagaat gagatatagt
     7801 ataagtgaca cagcagaata tggagattat gtatctggta aaagggtaat aactgatgct
     7861 gcaaaacaag gtatgcaaaa tgttttagag gatatacaaa atggaaaatt tgcaaaagcg
     7921 tggattaaag aaaatgaaga aggacgtgaa aacttcctaa aaactagaga agaagaatat
     7981 aatacagaaa ttgccgaagt tggtagaaat ctaagaagta tgatgtcatt cttaaaataa
     8041 atatatatta aatggattag tttgagggaa agagtggtgt atagtagtgc gtatgaatgg
     8101 agcaaaggta attttagaat gtttaaagaa agagggaatt gacacaatat ttggatatcc
     8161 tggtggagcc gtgatacctc tttatgatgc tctatacgat tattcagatg attttaaaca
     8221 tataaggaca tcccatgaac aaggtttagt tcatgctgca gatgggtacg caagaagtac
     8281 taatacagta ggagtttgtt ttacaacatc tggtccagga gcaacaaatg ccataactgg
     8341 aatagcaaca gcttttatgg attcttcacc tatggttgta atttcagggc aggtgccaac
     8401 tagtttatta ggaaaagatt cttttcaaga aattgacata actggagcaa cattatctat
     8461 gacaaaacat aattatttag ttagaaacac taaagaatta gttcctacaa taaaagaagc
     8521 ttttagagtt gcaaattcag gtagaaaagg acctgtactt gtagatgttc caaaagattt
     8581 atttttagca gaaatggatt ttagtggtga ggactatgat ttatgtcaaa tagatgatta
     8641 tatggattat aagagtgatt ttgatctaga tgatgaaact aatattaagc ttttaaatga
     8701 agcaatagat atcataaaag aatctaaaaa gcctgtaata tatgcaggtg gaggtgttaa
     8761 atcttcagac agtgaggaaa ttcttgaaaa atttgcaact aagatagata cacccgtact
     8821 aaatacactt atggggcttg gaaatataga tagaaagaat gaactatctc ttggaatggt
     8881 tggaatgcat ggaagtagag aaagtaattt agcactttct aattcagatt tagtaatagc
     8941 gataggtgcg agatttagtg atagagtgat aagtaaaagt tctgaatttg ctaaaaatgc
     9001 aaaaataata cacatagaca tagacccatc tgaaataagt aaaaatatag aatctaatgt
     9061 atctttggtt ggagatgtaa agctagtcct tagtttatta attgaaaggg ttgaaagtaa
     9121 gaataatagc aattggaaag aagagataaa gacatttaga aagagtgagg gtgtacaaac
     9181 atatgaattt catccacaaa atatacttaa aaaaataaat gaaaaatatg aaacattaaa
     9241 gaaacccact gtggtagtaa ctgatgtcgg tcaacatcaa atgtggtctg ctaaatactg
     9301 gaattttaaa ggtaataaaa gttttataac atcagcaggg ttaggaacta tgggatttgg
     9361 tttaggtgca gctattggaa ctaaagctgg taatgtagat aaaaatgtgg ttttagtaac
     9421 tggtgatgga agttttagaa tgaactgtaa tgaactagct acagttgcaa attataatgt
     9481 acctatgctt attttactac tcaataatag aacattagga atggtaagac aatggcagaa
     9541 gttattttcc aatcaaagat actctcaaac tgatattaat gaaaatgtgg attatgttaa
     9601 actcgtaaat gcatataata ttgatggata taaggtatcc aatatggaag aattaggaaa
     9661 ggttctagat gtgatagatt ttaataaacc tgtattttta caatgtgata tagataagga
     9721 ttatgatgtt tatcctattg tagcacctaa tgatgcattg gaaaatttga tatgtaacta
     9781 aaacttatac tgaatattga taaaattttt tattaaattt ttattggtat tcagtttttg
     9841 tttagagata gattggcatt ttttattttt gcaaataaat caatgaatat ataattaaga
     9901 acttattaaa ttttaattat acaatttaga atagtattac aacttaagta aatattaagt
     9961 tttattcgtt agatttatta ttaatacctt atattagtta tggttgttat atactctaaa
    10021 gccttgaaat tacaagcgta tagctgacta tataaatttg ataattttag cttttaaaat
    10081 agataaatta gattttgcca cccatttgcc accgtattat atttttgggt ggcaaactct
    10141 aatttgtaat tttttcaaat atatctacag tttcattttt cattttatca gttacatgtg
    10201 aataggtatc cattgtagtt gataattggc tatgacccaa acggttttgt atgtctttaa
    10261 tgttagcacc attttctaat aatagagtag catgtgcatg tcttaaagaa tgaaaatgga
    10321 agtcattgtt taaagctact cgaatttgtc ttactatagt gtctaaagtg tgagtattca
    10381 cttgttgacc attttctttg gtacataccc aatcactatc aaagtaaaat tctccgtatt
    10441 ttaatttcat tttcttttga tataatttat gttcttttaa tgcctttatt aaagtgtcac
    10501 ctgtaaatat agttctgcaa gagctttctg tttttggttg ccctaattca aacattccat
    10561 ttggtttttt aatcaaagta tgctttactg tgatagtttt attatcaagg tctatattat
    10621 cccattttag tgcaataatt tcacctcttc gcataccagt atgaaatcca attagtaaaa
    10681 ctatacgttg aaatgaatct tgaggaaata tatttagtat ttgattaaat tcttctaatg
    10741 taatagtttt aactttatta gtttctgttt tagatttagt ttttggtatg cttacatatt
    10801 gcatagggtt ttctcgtatg tgtttgtaag gatggactgc tgattttaat gacctatgta
    10861 atatggcttt taatacttgt aatgtatttt gagaataatc ctctttgtac tttttattta
    10921 tgaagttttg tagtattgca ggagttaaag cttttacttt gtaagctcct agttttggct
    10981 ttatatgttt ttctatgttt attcggtagc tttcttgagt gttgtattta cagttgagta
    11041 aaacatattc tttgtaccaa aagtctaggt agtctgataa gctgatgtta ctttcttcaa
    11101 agactatgcc agagttttca tattcattta gtgcttctct tagtgctttt tctgcttctt
    11161 tttttgtatt gccaccaact ctttctactt tttttctttt tccttctact atgcctaggt
    11221 caaagtagta ataccatttg ttgcttcttt ttcttactcc acctttcata atagtatccc
    11281 tcccttttag aatgtatgtt tgtttggtgt ttatataaaa aatgtctcct atatttacaa
    11341 gaagacatta attatattcg tcaaataata tttctaattt aacttttata cattcatcac
    11401 aattattttc tttgttaaca aatttaataa tgcaattata ctttaatcct ctatttatta
    11461 tacttgttaa ttctccacta taatatctag gaatgtaacc tatatgattc tcttttttat
    11521 caaatacctt gattgcatga ggatcaaatt tattttctgg ttctaactct agaactagag
    11581 cttcatttga ttctaattct attgaattat cacattcatg tccttcacac ccagcataat
    11641 gtcttactcc tgcaatataa aaatatctaa ttatattttt atcaatgtcc aaaggaattg
    11701 gatctacaaa ttctagtgta tctataggta atcttgcacc acttcttttt aaaagttcat
    11761 atgtatcata ctcagacata tcatacttat ccaaaatttt attgatgtcc tttctttttt
    11821 tatcaggtaa tctacttgca aatgggctaa ataaatcttc actataatat gttttactaa
    11881 tatcatcaaa tgatattaaa gcattaaaac ctgctttaat agcttcatca atttcatatt
    11941 cataagaaaa ttcatacaat ccatttttag ctaactttcc aattataaat tgtcttctac
    12001 tttttggatc tttccaaata acatataaaa aatcttttcc atttttaatt gacattttat
    12061 ccctctttca caaattatat atatttttta ttcttaacac tttttctttt agatatattt
    12121 ttagcaattt ttttaacttt gggcttataa tattttctga atagttatct attaatgtat
    12181 ttatgtttac ttcattcata agatttatta tcttttctac aaaatctata gtttgttcat
    12241 aataatttga atttaaatat tttagcactt ctaagtgtgt tggttctttt ttattttttc
    12301 catctatcct tattcttgat cttgaacgtg tatttataag agcttcaaat ctttgatgat
    12361 cagtgaagaa agaatctatg ttctcaatat taacataaca acataatgat gatccgttat
    12421 catatagagg actaagagta attttatttt gactttctag tagagcccaa ttattttgat
    12481 gcctatctct atttccaatc aagtaatcaa aaattaacat ttttagaaaa tcatttttta
    12541 tgttatattc ttctatagat ttaaaaatca ttttcaatga atagtattca ttatttttat
    12601 aatcatataa cttatctgta ttataacaag gataatattt gtttattaac cacacgcctt
    12661 caatcaattt ctcatcattt ttattgatta aataactcat tgaaccaatt cttttattat
    12721 atgtacctat ctcaacatta gcacaaggaa tctttaacaa actagctatt tcacaggcta
    12781 atttttctgc ccaatattct ccagtttctt ttgttttagg gaatttaaac aagcctatat
    12841 ctttagtttt ttcattaatt agccagattt tctcgcttct tccactacca taagaaaaac
    12901 catcatactc tttccattca tcaaaatttt taatcacgaa aataatcctc tcaaacaagt
    12961 attttatatt cttattgtat catttgattc taaatttgca agaattttat tatccataat
    13021 aagcttaact taaaataaaa attttttcta gattttattt attccacttt taacttcaag
    13081 ctttattaca tatggaatat gtgtttactc atcaaaatta ctactgtgta taatggtttt
    13141 actttataaa tacttaattt tagttttcta tatttatttg gtaatttttt tgagtgttgt
    13201 taagtaagaa atattttttg taccaaaagg taagctgata ttgctttatt cagatactat
    13261 gcctaggtca aagtagtaat accatttgtt gcttcttttt cttactccac ctttcataat
    13321 agtatctctc ccttttagaa tgtatgtatg tttggtgttt atataaaaga gcagattaac
    13381 tgctctttat atacttttgt tatgtacaat attatcttaa ttttgtttat ctggttttta
    13441 cttaattatt ttcttaatat tcttacaatt ttttagctt
//
LOCUS       DAMJWP010000085        13264 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.089, whole genome shotgun sequence.
ACCESSION   DAMJWP010000085 DAMJWP010000000
VERSION     DAMJWP010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13264
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.089"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(196..1077)
                     /locus_tag="PC700_002061"
     CDS             complement(196..1077)
                     /locus_tag="PC700_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HDF2581372.1"
                     /translation="MKITEGYMPFKGFKTYYRIVGENTEGKKPLVLLHGGPGSTHNYF
                     EVLDKIAESGRQVIMYDQIGCGNSFVEGHPELFNADTWIEELIELRKHLGLDEIHLLG
                     QSWGGMQAIWYAIEYKPKGIKSYILSSTLSSAKLWEKEQKRRISYMSEVDQKALLDAV
                     NTGDYSNKEYNDALERFMEMYCAGEVTEDSPECLRRPKKSGSEAYIVGWGQNEFSPTG
                     TLSGYEFTDRLHEIKEPCLVTSGAIDLCSPYIAKTMYDRIPNSKWELFEYSRHMPFVE
                     ENEKYIKVLTEWLNAND"
     gene            1533..2507
                     /locus_tag="PC700_002062"
     CDS             1533..2507
                     /locus_tag="PC700_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581373.1"
                     /translation="MNNINSLKVIKLSLGCSLAIFIAWLLKLEYSMVAGVIVLLTVKD
                     TKRETLKGSIGKIYGFLLCTIFSYLCFNILGYNLTSFSIFIFIIIPLCFLLNIQDVIA
                     MCVVIASHYYLQGETSIKWILNEAGIFAVGTGIGVLINMYIPTNIHKIHEVQKKLQEE
                     VSIVLIDIADIIVNPKKENGYSRDLYKLNSLIDSSISETYDNINNTLLSDTRFFLEHM
                     DIIKSQRDILENLYSYVSQLNSTPPQAHILSAFIHKIGYTEFEAETGNLLLEELKRLM
                     LSMKNHPLPVDRTEFENRAILFLCLTELKQFLVNRKHAQMLRDNNFYK"
     gene            complement(2623..3654)
                     /locus_tag="PC700_002063"
     CDS             complement(2623..3654)
                     /locus_tag="PC700_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HDF2581374.1"
                     /translation="MKKIRKLATKLLITTIVLVSGMSMTVYGMTAKEVTAKTPKSYVT
                     GTNSVYGPKLSQAQLNSVAQATADFMNNKITKNMTTDAKILVAYNHIKNNTTYVDWNA
                     VEGANTAYTLVTKKGACSGMARSMKALCDAMGIESYYVHSTSNDHQWNLIRFGDGRLY
                     HVDIDANKSAGKDIIYKSLSHPLPFDKTAYPTVGAVAQTQKPALPGKHTMLPHQDGAK
                     VVGKFGDIKDKHVMYEGVRCVVINSSVNTVTFLGSKSNLDPSNLTIAVIGFMNTPYGM
                     FALAPEGEPVEYPFKYDKPFSLDFSGKNRSGAELAKYCKKNDVTIKIEITDEKANIGL
                     GQIMYIRYE"
     gene            complement(4266..5117)
                     /locus_tag="PC700_002064"
     CDS             complement(4266..5117)
                     /locus_tag="PC700_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2581375.1"
                     /translation="MSCILKLKQIFEDLTEVDKKIADYILNNTEAISKLSVSELASNS
                     KTSTASIVRFSRKMGYSGFGDLKIEIAKDLMGKENEYTYVDENYDCNIDSVINKITNK
                     NIETINQTRLLNEKDIIKEAVEQIIKAKNVYIFGVGGSALVALDLQMKLLRINKQAFT
                     SLDSHTQLMVSSNVDKEDIAIAISYSGESKEVIKSIENAKLKGCKVICITKYSDNHLS
                     KISDLKLVVPNIEKRLREGAISSRIAMLTLIDIIYISIIQENLNEAEQKLQETKRILD
                     YLNINNA"
     gene            complement(5134..6054)
                     /locus_tag="PC700_002065"
     CDS             complement(5134..6054)
                     /locus_tag="PC700_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HDF2581376.1"
                     /translation="MYFLGVDGGGTKTTFTLVDEELNIVGTITKGTCHYNQIGFDNLT
                     KLLITGLEEVCKDAKINVEEITYAFVGLAGYGKIKEVLYALEVATKNAYSHINYTLGN
                     DVEIALAGSLNGEKGINIIAGTGSIAQALDKDGNLHRCGGWGYVLGDEGSAYYIGMAT
                     LKMFTMQSDGRCSKTKLYDLIKRHLNIENDYDIIKYVNDGIQGDRIEIAKFATICLKA
                     VIEGDNTASKIFDDAAYELSRLIIGLEHHFEQGTKIKVSYSGGVFKSGDLILEPLKKY
                     LNKTRFGIVRPVLTPDLGACLLAKKKYYSK"
     gene            complement(6164..7078)
                     /gene="murQ"
                     /locus_tag="PC700_002066"
     CDS             complement(6164..7078)
                     /gene="murQ"
                     /locus_tag="PC700_002066"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDF2581377.1"
                     /translation="MLIKTLENLVTEGRNKNTLQIDKEDTLGIIELINNEDKTVAYAV
                     EEQKESIAKAVNIIVDRMKQGGRLFYIGAGTSGRIGILDATECPPTYGVDFELVQAII
                     AGGNQAIFKAIEGAEDDKELGKQDIIDRGVTSKDVICGIAASGRTPYVIGAMEYAKEL
                     GCAVLSITMNPNSEMSKKADLPINIIVGAEVIMGSTRMKSGTAQKMVCNMLTTASMVK
                     MGKVYSNLMVDVKTSNEKLVERAKRIIMIATNVKYDVAEKFLEEADNRVKLAIFMIKS
                     GLDKDSAKSILDRQEGYISEALKSIEKL"
     gene            complement(7177..8274)
                     /locus_tag="PC700_002067"
     CDS             complement(7177..8274)
                     /locus_tag="PC700_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDF2581378.1"
                     /translation="MGRLGISIYPEKTTSKEIYDYIDKAAENGFTRIFSCLLSVKDTK
                     ENIIKKFKSINEYAKFKGFEVILDVNPRVFGELEISYKDLTFFKKTKADGIRLDMGFT
                     GSEKALMTFNPQNLKIEINMSNNTNYIDTIMDYQPNKENLTGCHNFYPHRYSGLNLEH
                     FIKCTERFNKYGLRTAVFVTSQNETTFGPWPVTDGLPTIEMHRNLPIDIQVKHIIAMN
                     NINDIIISNCYPTYEELKVLGSMRKDMVTFDVAIEENIREVEKKILFEEIHFNRGDIS
                     DSLIRSTQSRVKYKGYKFELFNTPKIIKRGDIVIESSEYGHYAGELQIALTDMKNSGK
                     SNVVGYIRKEELFILDYIKPWQKFDFKPSTK"
     gene            complement(8507..9853)
                     /locus_tag="PC700_002068"
     CDS             complement(8507..9853)
                     /locus_tag="PC700_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2581379.1"
                     /translation="MLDKFTEFLDSKLSTSMARLAEQRHLRAVRDGIVASLPLIIVGS
                     FFLILAFPPLPDSWGVTQYLKENAVTILLPYRMTFFIISVYSTFGIGYSLAKSYKLDG
                     LTGGLLATATFLLTIVPTAIEGLTPDLLKIVEKSPELLAWYNSIPQGWQMPMANLGGG
                     SMFVAIVTSIIGVEIYRFTDKSKFKITMPEAVPASVTRSFEALTPAALIIILMAIVTY
                     FLGFDWHGFIAKLVTPLVSATDTLPSVIILIFLITFFWSFGIHGVSIIGSLARPLWIQ
                     LLDANTASYAAGQAVPGIAAEPFFQWFIWIGGSGATIGLALLLAFRAKSAYGKSLGKA
                     TVIPAIFNINEPIIFGAPIVLNPILIIPFVVAPIVMATVSWFAMSANLVSRVVVTAPW
                     TLPGPVGAYLATGGDWRASVLNLLCIAISIIIYYPFVKMYDKRLLHEEKVEEQEAY"
     gene            complement(9942..10250)
                     /locus_tag="PC700_002069"
     CDS             complement(9942..10250)
                     /locus_tag="PC700_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2581380.1"
                     /translation="MRVLFVCSSGMSSAIAVNALQKEGAKNGIDIDVLAVGTQEFENE
                     VKNGWDIAMVAPQVRHRFDYLKAFADEASVPCALIQAQAYSPLGGPKLLKQVQELLSK
                     "
     gene            complement(10511..10900)
                     /locus_tag="PC700_002070"
     CDS             complement(10511..10900)
                     /locus_tag="PC700_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581381.1"
                     /translation="MSNNKKKKRKKNNNYKNAKSNKNTKKEMEKNQRTDYNIPTISFK
                     SGLILIVSSLVSTVVFPLLLSIFGIDNRFGVVIGNSLITSFAVAYTRYFVETKRGFSL
                     GFFRLYILFVLSFAIIGYFWIYKGIHI"
     gene            complement(10902..12062)
                     /locus_tag="PC700_002071"
     CDS             complement(10902..12062)
                     /locus_tag="PC700_002071"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HDF2581382.1"
                     /translation="MITMYSVGLMSGTSLDGVDAVLAEISGNGRNTKVKQIEFITLEI
                     PKDIKDEIRKCCIEEESSVDLICSLNFKLGYLFSKAVKSVCHKANFHIANLDFIASHG
                     QTIFHIPRSYNNFVSSTLQIGEPAIIAYETNTKVISNFRVMDIAAGGEGAPLVPYSEF
                     LLYSDKNKNLALQNIGGIGNITIIPKSCNIDDVFAFDTGPGNMIIDGVCQRLFNRKYD
                     KNGYFASKGKINEEMLKDLMSHKYLSQAPPKSTGREVFGQVYLDNMLNKYKHVDKYDL
                     IATVTMFTAKAIYYNYKNFILPKVNVDTLLIGGGGVHNLTLIGYIKELLLEVEVLTQD
                     EYGYSSDAKEALAFVILGNETLNNSFSNVISATGAKNKVILGNITPKPFGGK"
     gene            complement(12492..>13264)
                     /locus_tag="PC700_002072"
     CDS             complement(12492..>13264)
                     /locus_tag="PC700_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2581383.1"
                     /translation="IDGQSFEENLIKDIIENLKIKNNSDIETYKQSLKELDDYIAKWV
                     HEIKIPISSLSIITDRLSSIEDSLDIKNQVAKINFLVNSILYSSRSNTMFEDVFINKF
                     NLEKLVKMSIKNNSFLLIKNNVEVSLNELENDVYTDSKCMSYVLDQIINNAIKYSKEV
                     GKIEFNSKKLENGVVLSIKDFGIGINEEDISRVFDKGFTGKNGRNQLYKSTGMGMYFV
                     KKMIDSLGHEIEVCSKIGSYTIFNIYFYDISDYLSLDS"
BASE COUNT         4254 a         2343 c         1461 g         5206 t
ORIGIN      
        1 ctattatttc aataagtata caattagatt agaatctaac gctagtaata aaaaattact
       61 ttcccaaaat agatataagc ctcattttat cgttcaaata atgaaaatat atttgatttc
      121 atgcaaaaag agtgcctaat gaactaaaaa gttcattttg agacactcat ttctttatat
      181 aaatttatat agtttttaat cgttcgcatt caaccactca gttaagactt ttatatattt
      241 ctcattttct tcaacgaatg gcatatgtct tgaatactca aataactccc acttactatt
      301 aggtattcta tcatacatag tctttgcaat atatggcgaa cataaatcta ttgctccact
      361 agttactaga caaggctctt ttatttcatg taatctatct gtaaattcat atccagaaag
      421 agttcctgta ggagaaaatt cattttgtcc ccatcctaca atatatgctt cagaacctga
      481 ttttttaggt cttctcaggc actctggaga atcttctgtc acttcacctg cacaatacat
      541 ttccataaat ctttctaatg catcattata ttctttatta gagtaatctc cagtatttac
      601 tgcatcaagc aatgcttttt ggtcaacctc actcatatat gatattcttc ttttttgttc
      661 tttctcccaa agttttgcag aagaaagagt agatgaaagg atgtaagact taataccttt
      721 tggtttatat tcaatagcat accaaatagc ctgcatacct ccccaagatt gacctaacag
      781 atgaatttca tcaagtccta gatgttttct tagttcaata agctcctcta tccaagtatc
      841 tgcattaaat aactcaggat gcccttctac aaaggaattt ccacaaccaa tttggtcata
      901 cattattact tgtctaccac tttcagctat cttatccaat acctcaaaat agttgtgtgt
      961 agaacctgga cctccatgaa gtaatactaa tggcttcttc ccttccgtat tttctcctac
     1021 tatacggtaa tacgttttaa aacctttaaa aggcatatat ccttcagtaa ttttcatatt
     1081 tatctcctcc aatttgtaat tataaacaat aatcaagttc attaacaata ctatttaaaa
     1141 cctctcatac gtatttaatt atgtctttta atctttatac ctacttttat aaatagtata
     1201 acataaaagt taaaaatctc tatactaaac caattataaa attatattaa ctatataatt
     1261 ttatttttta ataatagtat atacatattt gattattttc atttgctatt taaaatcaat
     1321 tttaagtttt cttcttataa taaacttcct ctttgtggtc tcatgttagt ctctaaattt
     1381 aaaattttac aaaaaatttt cataaaataa aattatattg attgtatcta aacaaatagg
     1441 gcaataaaat aattaagtat agttacttac ctttcaagca atattagttt tatgttagaa
     1501 ttttattata ttgatttaca ggaggtttgc atttgaataa tattaattca cttaaagtaa
     1561 taaaattatc tcttggatgt tctcttgcta tattcattgc ttggctatta aaattagaat
     1621 attcaatggt tgctggagtt atagtactac ttacagtaaa ggacactaaa agagaaactc
     1681 ttaaaggctc cattggaaag atttatggtt ttcttttatg tactattttt tcatatttat
     1741 gttttaatat tttaggatat aatttgactt cattttcaat atttatattt ataattatac
     1801 cactttgttt tttgttaaat attcaagatg ttattgctat gtgtgtagtc atagcatccc
     1861 attactacct tcaaggagaa acttctatca aatggatttt aaatgaagct ggaatatttg
     1921 cagtaggaac tggaataggt gtcttaataa atatgtatat tccaacaaat atacataaaa
     1981 tccatgaagt tcaaaaaaag ttacaggaag aagtgagtat tgtacttata gacattgcag
     2041 atattattgt aaatcctaaa aaagaaaatg gatatagtag agatttatat aaattaaatt
     2101 cacttattga cagcagtatt tctgaaactt acgataatat taataatact ttattaagtg
     2161 atactagatt cttcttagaa catatggata ttataaaaag ccaacgggat atcttagaaa
     2221 atttgtatag ttatgtatct caactaaatt ctacaccacc acaagctcac attctatctg
     2281 ctttcataca taaaataggt tatactgaat ttgaagctga aacaggcaat cttcttcttg
     2341 aagaattgaa gagattaatg cttagtatga aaaaccatcc tctacctgtt gacagaacag
     2401 agtttgaaaa cagagctatt ttattcctat gcttgactga gttaaaacaa tttttagtta
     2461 atcgtaaaca tgctcagatg cttagagata ataattttta taaataaaaa ggtgagaaat
     2521 ccagcagttg acctgactac aaaatatcag atataatatt aaatgcgccc ctagtaagag
     2581 ggcgcgatgt ttataactta ttctactgtc atgattaaat cattattcat atcgaatata
     2641 cattatttga ccaagtccaa tattagcttt ttcatcagtg atttctattt taatagtcac
     2701 atcatttttt ttacagtact ttgctaattc tgcaccagaa cgatttttac cactaaaatc
     2761 taaagaaaat ggcttatcat atttaaaagg atattcaaca ggttcacctt ctggtgctaa
     2821 tgcaaacatc ccatatggtg tgttcataaa tcctattaca gcaattgtaa gatttgatgg
     2881 gtctaaattt gatttactac ctagaaatgt aactgtattc actgatgaat taattacaac
     2941 acatctaaca ccttcataca ttacatgttt atcctttata tctccaaatt ttccaacaac
     3001 ctttgccccg tcttgatgtg gtaacatagt gtgcttccca ggtagtgctg gtttttgcgt
     3061 ttgtgctact gctccaactg ttggatatgc tgtcttatca aatggtaatg ggtggctaag
     3121 actcttgtat atgatatcct ttcctgctga tttattagca tcaatatcta catgatacaa
     3181 acgcccatca ccaaaacgta taagattcca ctggtggtca tttgatgtgc tgtgtacata
     3241 ataagattca attcccatag cgtcacacaa agctttcatg ctcctagcca ttcctgaaca
     3301 tgcacctttt ttagtaacca atgtataggc agtattcgct ccttctactg cattccagtc
     3361 aacatatgtt gtgttatttt taatgtgatt ataggcaact aaaatcttag cgtctgtagt
     3421 catgttcttt gtaattttat tgttcataaa atctgctgtt gcttgtgcta ctgagtttag
     3481 ttgggcttgt gacagctttg gaccatatac gctattggtt ccagtgacat agctttttgg
     3541 tgtttttgct gttacctctt tggctgtcat accataaact gtcatactca tacctgagac
     3601 taaaacaatt gttgtaatca ataacttggt agctaacttc ctaattttct tcatacttag
     3661 tattctcctt tataaaatat tgttacttcc tattctctac cattaataaa gttcatttgg
     3721 gatttaaaaa ggtggattgt caatatgtat gtttcttcat ttatacgttc gtgaaatatt
     3781 taaatttcat ggaaaatcta atagatattt tctagataat ttgtatacaa tacaactcgc
     3841 aaatcagggt gtttgctgtt agtttgagaa aatgatgatg attaatatat tcggaatctt
     3901 tgacctataa taaataccat tgtcttcaat cattaataat ggtaagagta aagagaatta
     3961 tcaataatac agttaaaatc aactttgtat acatttcata cattctctcc caacaacatt
     4021 atacttctta taatttgtat attcaatacg ttttctggga aaatatagga tatatctata
     4081 aaaatataaa aaaggagttg aacttaaaat ttgattaaaa aaaatcaatt ttaagacaac
     4141 tcatttgtat tataaaaatg ctatatatta ttattaagta agtcaatatt ttagatattt
     4201 atctatttct tattgactta cgcttatagt ctaacttatt tgtttagttg atatataacc
     4261 tagttttaag cattattaat atttaaataa tcaagtatcc tttttgtttc ttgtagtttc
     4321 tgttctgcct catttaagtt ttcttgaatt atagaaatat atattatatc tatcaacgta
     4381 agcattgcta ttcttgaaga aatagctcct tctctaagcc ttttttctat atttggtact
     4441 actagcttta aatcactaat cttactaaga tgattgtcac tatatttagt tatacaaatg
     4501 accttacatc cttttaattt tgcattttct atagacttta ttacttcttt tgattctcca
     4561 gagtaagata ttgctatagc tatatcttct ttatctacat ttgaagatac cataagttgt
     4621 gtatgactat ctaaagaggt aaatgcctgt ttatttatcc ttaaaagttt catctgtaaa
     4681 tctagtgcaa ctaaagccga accacctact ccaaatatat atacattttt agcctttatt
     4741 atctgttcaa ctgcttcttt tattatatct ttttcattca acaaccttgt ttggttgata
     4801 gtctctatgt ttttgtttgt aattttgttt ataacactat ctatattaca atcatagttt
     4861 tcatctacat atgtgtattc attttctttg cccatcaaat ctttagctat ctcaattttt
     4921 aaatccccaa aacccgaata tcccattttt ctagaaaacc taactatact tgctgtactt
     4981 gtttttgagt ttgacgccaa ttctgataca gaaagcttac ttatagcctc tgtattatta
     5041 agtatgtaat cagctatttt tttatctact tccgttaaat cttcaaatat ctgttttaat
     5101 tttaaaatgc aactcatact ctaccacctc tacctattta ctataatatt tcttcttagc
     5161 aagtaaacat gctcctaaat ctggagtcaa cactggtctc actataccaa atctcgtttt
     5221 attcaaatat tttttaagtg gctctaatat taaatctcct gacttaaaca ctccacctga
     5281 ataagatacc tttatctttg taccttgttc aaaatgatgc tctaatccta ttataagcct
     5341 acttaattca taagctgcat catcaaatat tttactagca gtattatcac cttcaataac
     5401 tgcttttaaa catatcgtag caaactttgc tatttctatt ctatctcctt gtattccatc
     5461 atttacatat tttattatgt cataatcatt ctctatatta agatgtcttt ttattaaatc
     5521 atataatttt gtctttgaac accttccatc agactgcatt gtgaacattt ttaaggtagc
     5581 cattcctata taatacgctg aaccttcatc tcctaataca tagccccatc ctccacatct
     5641 gtgaagattc ccatctttat ctaaagcctg tgctattgaa ccagtacctg caattatatt
     5701 tatacctttt tccccattta aagaacctgc taatgctatt tctacatcat tacctagagt
     5761 ataatttata tgactatatg cattttttgt tgctacttct aatgcataca atacttcttt
     5821 tatttttcca tatccagcaa gtccaacaaa tgcatacgtt atttcttcta catttatctt
     5881 agcatcttta caaacttctt ccaacccagt tattaataat ttcgttagat tatcaaatcc
     5941 tatttgatta taatgacaag ttcctttggt tatggttcct actatattta attcttcatc
     6001 tactaatgta aatgttgttt tcgttcctcc accatctaca cctaaaaaat acatactatc
     6061 ctcctacaat atctttaata attctttcaa tacttaatat ctaaaaataa ccttccaatg
     6121 ctgttttttt aatattgaaa ggttatcagt actattattt attttacaat ttttctattg
     6181 attttaaagc ttcacttata taaccttctt gtctatctag gatactcttt gcactatcct
     6241 tatctaaacc actttttatc ataaatatag ccaatttaac tctattatct gcttcttcta
     6301 agaatttttc tgcaacgtca tattttacat ttgtagctat cattattatt ctttttgctc
     6361 tttcaaccaa cttttcattt gatgtcttaa catctaccat caagtttgag tatacttttc
     6421 ccatcttaac catagaagct gttgttaaca tattacatac cattttttga gctgtaccac
     6481 ttttcattct agttgaaccc attattactt ctgcccctac tataatgttt attggtaaat
     6541 ccgctttttt actcatttca ctattcggat tcatagttat tgatagtact gcacacccca
     6601 attccttagc atactccatt gcacctataa cataaggagt ccttcctgat gcagctatac
     6661 cacatataac atccttagat gtaactcctc tatctattat atcttgcttt ccaagttctt
     6721 tatcatcttc agcaccttct attgctttaa atattgcttg atttccacca gctattatag
     6781 cttgaacaag ctcaaaatcc actccgtaag ttggaggaca ttctgtggca tctagtattc
     6841 ctatcctccc actggtacct gctcctatat agaataatct acctccctgc ttcattctat
     6901 ctactattat attaacagct tttgctatac tctccttttg ttcttcaaca gcataggcta
     6961 cagttttatc ttcgttattt attaattcta ttatgcctaa tgtatcttct ttatctattt
     7021 gcaatgtatt cttatttcta ccttctgtaa ctaaattttc taaagttttt attaacatat
     7081 catttcctca ttttcttaat attaaatttt taaacacatt ttaactccaa ctttcataat
     7141 tatattttta taataaatta atattttcat aataaattat tttgttgagg gtttaaaatc
     7201 aaacttttgc catggtttaa tataatctaa tataaataat tcttcttttc ttatatatcc
     7261 tactacattc gatttaccac tgtttttcat atcagtcaag gctatttgta actcccctgc
     7321 ataatgacca tattcactac tttcaattac tatatctcct ctttttatta ttttaggagt
     7381 attaaatagt tcaaatttgt atcctttata tttaacacga gattgagtag accttatcag
     7441 actgtcagat atatcccctc tattaaaatg tatttcttca aagagtatct tcttctcaac
     7501 ttctcgtata ttttcctcta ttgctacatc aaatgtaacc atatcttttc tcatagaacc
     7561 taataccttt agctcctcat atgttggata acaatttgat attattatat catttatatt
     7621 attcatagct attatatgct ttacttgaat atctataggt agatttctgt gcatctcaat
     7681 tgttggaagc ccatcagtta caggccatgg tccaaatgtg gtttcatttt gacttgttac
     7741 aaaaactgct gttcttaatc catacttatt aaatctttct gtacatttta taaagtgttc
     7801 taaatttaat ccactatatc tatgtggata aaagttatga catcctgtca aattttcttt
     7861 atttggttgg tagtccatta ttgtatctat ataattagta ttattactca tattgatttc
     7921 tattttaaga ttttgaggat taaaagtcat taaggccttt tcagaacctg taaatcccat
     7981 atcaagcctt attccgtctg ccttcgtctt tttaaaaaaa gtcaaatctt tgtagcttat
     8041 ttctaattca ccaaatactc tgggatttac atcaagtatt acctcaaaac ctttaaactt
     8101 agcgtattca tttatacttt taaatttttt aattatattt tctttagtat ctttaactga
     8161 taataaacat gaaaatatac gtgtaaatcc attttcagca gctttatcta tataatcata
     8221 tatttcctta gatgttgttt tttctggata aattgatatt cctaatcttc ccatctgtgt
     8281 catctcctta tatcctatat gtgtttaaat ttatttcgaa ttacacgaaa cttttttaaa
     8341 aatttggtga aaaaaagttt catatatatt tataaaaaaa gaaaagttta ccttttcttt
     8401 ttttataaac aatcaacaaa atatcttcgt tacttatttc ataactgtta tactttgaaa
     8461 tttatactaa tctaatatag tttttaattt ccaatatagt ttctaattaa taagcttctt
     8521 gctcttcaac tttttcttca tgaagcaatc tcttatcgta catcttgaca aatggatagt
     8581 aaattattat tgatatagct atacataata agttaagaac tgaagctctc caatctccac
     8641 cagtggctaa atatgcccct actggtcctg gtaatgtcca aggagcagta actactactc
     8701 tggatactaa attagcactc atagcaaacc atgaaactgt tgccattact attggtgcca
     8761 caacaaatgg tattattaat attgggttta atactattgg tgctccaaat attattggtt
     8821 catttatatt gaatattgct ggtattacag ttgcttttcc taaagacttt ccataagctg
     8881 attttgctct aaatgcaaga agtaaagcta agcctattgt agctccagaa cctccaatcc
     8941 atataaacca ttgaaaaaag ggttcagcag caatacctgg aactgcttga cccgcagcat
     9001 atgaagctgt attagcatct aataattgta tccataaagg tcttgccaat gaacctatta
     9061 ttgatacacc atgtattcca aatgaccaaa aaaacgttat taaaaatatt agtataatta
     9121 ctgatggtaa tgtgtcagtg gctgaaacta atggagttac taatttagct atgaagccat
     9181 gccaatcaaa ccctaggaaa taagtaacaa tagccattaa aattattatt aatgcggctg
     9241 gtgtcagagc ttcaaaagac cttgtgactg atgctggaac tgcttctggc atggttatct
     9301 tgaatttaga tttgtcagta aatctataaa tctcaacacc tattatagat gttacaatag
     9361 caacaaacat tgaacctcca ccaagattag ccattggcat ttgccatcct tgaggaattg
     9421 aattatacca agctaaaagt tctggactct tttcaactat ctttaataaa tcaggggtaa
     9481 gaccctctat tgcagtagga actattgtca aaaggaatgt agctgtagct aataaacctc
     9541 ctgtcaatcc atctaactta tatgactttg ccaaactata acctatgcca aatgtagaat
     9601 atactgatat aataaaaaat gtcattctat atggtagtaa tattgtaact gcattttcct
     9661 ttagatactg agttaccccc catgaatctg gtaatggagg aaatgctaat atcaagaaaa
     9721 atgacccaac tattataagt ggtaatgaag ctacgattcc atctcttaca gctcttaagt
     9781 gacgttgttc tgccaatctg gccatagaag ttgataattt actgtctaaa aattctgtaa
     9841 atttatctag catcttttct ctccttaatt aatatttatt tttattatca ttaaaatttt
     9901 tataaaatta actttcttct aacctaacaa cactgcaact actatttact caacaactct
     9961 tgaacttgtt ttaataactt tggtcctcct aatggactat aagcttgtgc ttgtattaat
    10021 gcacatggta cactagcctc atcagcaaaa gcttttaaat agtcaaatct atgtctgact
    10081 tgtggagcta ccatagcaat atcccatcca tttttaactt cattctcaaa ctcttgagta
    10141 ccaacagcca aaacatctat atctattcca ttttttgctc cttctttttg taatgcatta
    10201 actgctatag cacttgacat ccctgatgaa caaacaaata atactctcat attgattccc
    10261 cctgtttttt tatttctttt caatcattat agcatgtatt tttgattaca ggaatacgtt
    10321 tgtatatatt ttttcatatt ttacattttt aattgtaatt ttatttcatg catttatatc
    10381 ttgctttaat tgctttattt atctaatcat tcttttgtct aaaattacac actacttaaa
    10441 atactcatat cttttcttaa aaaagaaaaa agactatctt ttattaacat aaaatagtct
    10501 caatactttt ttaaatatgt attcctttat atatccagaa gtaacctatt attgcaaatg
    10561 ataaaacaaa taaaatatac agtctaaaaa atcccaaaga aaaacctctc ttagtttcaa
    10621 caaagtatct tgtatatgca actgcaaaag atgtaattaa tgaatttcct ataacaactc
    10681 caaatctatt atctattcca aatatagata ataacaaagg aaaaactaca gtacttacta
    10741 gagatgaaac tattagtatt aatccacttt taaaacttat agttggaata ttataatctg
    10801 ttctttgatt tttctccatt tctttcttag tatttttatt actttttgca tttttataat
    10861 tattattttt ctttcttttc ttttttttat tattagacat gtcatttacc tccaaaaggt
    10921 ttaggtgtta tattccctaa aataacttta tttttagccc ctgttgcaga tattacatta
    10981 gaaaaagaat tatttaatgt ttcattgccc aagattacaa atgccaaagc ctcctttgca
    11041 tcagaagaat acccatattc atcttgagtt aatacttcaa cctctaataa taactctttt
    11101 atatatccta ttaaagttag attatgaact cctccacctc caattaataa agtatctaca
    11161 ttaacctttg gaagtatgaa gtttttataa ttataatata tagcttttgc cgtaaacata
    11221 gttactgttg ctatcaaatc atatttatct acatgtttat atttgttaag catgttatcc
    11281 aaatagactt gtccaaatac ctctcttcca gtgcttttag gtggcgcttg actaagatac
    11341 ttatgactca tcaaatcttt taacatttct tcattaattt taccttttga tgcaaagtat
    11401 ccatttttat catactttct attaaataat ctttgacata ctccatctat tatcatattt
    11461 cctggtcctg tatcaaatgc aaatacatca tctatgttac atgatttagg tattatagta
    11521 atatttccta ttccaccaat attttgaagt gctaaatttt tatttttatc actatataat
    11581 aaaaattcac tatatggtac taatggagca ccttctccac ctgctgctat atccattact
    11641 ctaaaattag atattacctt tgtatttgtt tcatatgcta taattgctgg ttcacctatt
    11701 tgaagcgttg aagatacaaa attattataa cttcttggta tatgaaagat tgtttgacca
    11761 tgagatgcga taaaatccaa atttgctata tgaaagtttg ctttatgaca cactgatttt
    11821 acagctttac taaataaata tccaagctta aaatttaagc tacagattaa atcaacactc
    11881 gattcctctt ctatacagca ctttcttatt tcgtctttta tatctttagg aatttctaga
    11941 gttataaatt ctatttgctt aactttagta tttctaccat tcccactaat ttcagctagt
    12001 actgcatcaa ctccatctaa ggaagttcct gacattaaac ctacactata catagttatc
    12061 atccttttaa gtttatagtt attcctcccc aaatcaaggt attaagttta ataattttag
    12121 aattaatctt atttcatctt taattgcgat aatttactct tacgaaatta atcgagttaa
    12181 tcttatacac gttttttacc ttagctaaat cttacattaa tttattttca gtgtcaatgt
    12241 aatgcatgta attttttttc acaaacaaaa catattctct ctccaaaaac catattaaaa
    12301 tagttactac aaataaaaaa cataccaggt acctatctga tatgtttttc atatataata
    12361 attattattt tataatatag atagaacatc atttaaggca ctaattaaat cttcactatt
    12421 ttcaagaact aaatttattc ttattgtata tgtcgatttg tatatgatga tagattttat
    12481 agtctaataa cctaactatc taggcttaaa taatccgata tatcataaaa gtagatatta
    12541 aaaattgtat atgaaccaat tttcgaacat acctcaatct catgccctaa actatctatc
    12601 attttcttta caaaatacat ccccattcca gtagacttat acaattgatt tcttccattt
    12661 ttccccgtaa atcccttgtc aaaaacccta gatatatctt cctcatttat acctatacca
    12721 aaatctttta tagataatac cactccattt tctaactttt ttgaattaaa ttcaatctta
    12781 ccaacttcct ttgaatactt tatagcatta ttaataattt ggtctaaaac atatgacatg
    12841 catttagaat ctgtgtaaac atcattttct aattcattca aactaacttc tacattattt
    12901 tttataagca aaaatgaatt attttttata gacattttta ccaatttttc taaattaaat
    12961 ttatttataa atacatcttc aaacatagtg ttgcttctac tactatacaa aatagagttt
    13021 actaaaaaat ttatctttgc aacctgattc ttaatatcca aactatcttc tatgctactt
    13081 aacctatctg taattatact taaagaagat attggaattt ttatttcatg aacccattta
    13141 gctatgtaat catctaattc ttttaatgat tgtttataag tctctatgtc actattattt
    13201 tttattttta agttttctat aatatctttt attagatttt cttcaaaaga ttgtccatca
    13261 atta
//
LOCUS       DAMJWP010000086        13258 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.090, whole genome shotgun sequence.
ACCESSION   DAMJWP010000086 DAMJWP010000000
VERSION     DAMJWP010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13258
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.090"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            87..323
                     /locus_tag="PC700_002073"
     CDS             87..323
                     /locus_tag="PC700_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HDF2581384.1"
                     /translation="MLEISTDLQVNQPVITVTSNTFISIENYLSILEYEVDLIRIKTK
                     VKTIKISGDKLSLKYITDSEIGIKGIIYNVEYVD"
     gene            336..1679
                     /gene="yqfD"
                     /locus_tag="PC700_002074"
     CDS             336..1679
                     /gene="yqfD"
                     /locus_tag="PC700_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HDF2581385.1"
                     /translation="MISFIRGYYVIVVEGVGLEQFLNHLIRNGISVYNVTRIKNTKME
                     FHIDRQDIKEFKNVYRGSKFDIKVKQKTGVPFIIKRVYKHKGMWICALVSLFLLMSTS
                     QFVTDVYIQSPEGIKKEALRNELYKVGVRPGVYKKSIDRKEVRDHMMSKFNDVAYLSI
                     NVKGTNIFVTVTKKAESLKSTDQSNYCNVIALKNGIIEKVIPRSGKSVIKSGDIVQKG
                     DVLLNGANTKSIPEVWASTFYESTKKASYVDTVNKKTGEKKNIYTLSFYDKEFTMRKN
                     IKYKDYVVENKEKKLSIGNYTFPIKIKTSTFYETKKVEVKRNKEELKKELSEKALKEL
                     EYIIPASARIIDVKHNFKVNKNMLEYLITVQTSENIAKIYPLSKSEAERFIKEESKPD
                     EGEEEVPSNPEKRPLNDIRNEFDEDNKDNKDQNNSDENNSNQNSNNSQNNNSNNN"
     gene            1618..2733
                     /locus_tag="PC700_002075"
     CDS             1618..2733
                     /locus_tag="PC700_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDF2581386.1"
                     /translation="MKIIVIKIVIIVKIIIAITTNKIKLKILGGLIQLIIQKKFTIAD
                     GNFERELFGNFDENVKLIEKTLNIDVILREGNIILIGEEKNVDSALKLMNELHQTVSN
                     GKHLDKQSISYSLSLLLEGSEQKIKELEGTIVITQRGKAVQPKTLGQKEYIKLIENND
                     ITFGVGPAGTGKTYLAVAMAVKAFKRDEVSRIILTRPAVEAGESLGFLPGDLKDKVDP
                     YLRPLYDALFEMLGADKFNKYLERGTIEVAPLAFMRGRTLDNSFIILDEAQNTTSEQM
                     KMFLTRLGFGSKAVVTGDVTQTDLPQNKKSGLIQATEILKGVPGIGSIMLTDRDVVRH
                     ELVQRIIRAYEKHDKREEFKKEERKKVKMQEKTFKRK"
     gene            2790..3251
                     /gene="ybeY"
                     /locus_tag="PC700_002076"
     CDS             2790..3251
                     /gene="ybeY"
                     /locus_tag="PC700_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDF2581387.1"
                     /translation="MDLILDDRQDKLEVSEELIEKIKDIIIECLDYEGYDDNYEVSLS
                     FVDNKEIHELNREYRGVDRVTDVLSFPLLSDDFEDVELEEESLGDIVVSLERALEQSI
                     EYNHSFEREVCFLICHSMFHLLGYDHDTDENTKEMREKEEHILNKLNITRE"
     gene            3257..3976
                     /locus_tag="PC700_002077"
     CDS             3257..3976
                     /locus_tag="PC700_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDF2581388.1"
                     /translation="MKPEKTRQGIIKAFNAAIEGILYTFKFERNMKIHYLGSVAVLII
                     SLFFNFSKLEMIMLLMSICLVVVAEMFNTAIEKAVDLVTDEYHVLAKIAKDVAAGGVL
                     VAALNSVVVGYILFYDKLTDISGILIYKIRESELHITLICILLVLIAVVVVKALTSTG
                     TPLKGGMPSGHAALAFAIATAITLMTERVVASTLAYIMAVLVAQSRIEGKIHTFWETI
                     AGALLGVLIAILVFQLGMFYN"
     gene            4095..4496
                     /locus_tag="PC700_002078"
     CDS             4095..4496
                     /locus_tag="PC700_002078"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDF2581389.1"
                     /translation="MDNIELLRLAEDARQHSYAPYSGFRVGAALLTKSGKVYTGCNIE
                     CASLGGTNCAERTAIFKAISEGDKDIYKIAIASDNSENNEQTYPCGICRQVIIEFGSD
                     IKIITGYTKGEVFEHSIKDLLPNYFSGNDLK"
     gene            4560..5453
                     /gene="era"
                     /locus_tag="PC700_002079"
     CDS             4560..5453
                     /gene="era"
                     /locus_tag="PC700_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDF2581390.1"
                     /translation="MFKSGFVSIVGRPNVGKSTLMNNVVGEKIAIMSDKPQTTRNTIQ
                     AVYTDEEMQIVFLDTPGIHKPKNKLGEFMVKAATEAFKNVDLILFVVDDSKKIGPGDR
                     KIIEDLRSVKTPIILVVNKIDQLDQKDELFDIIKMYDREGIFKEIVPISALKGKNTDT
                     LIKVIQNYLEEGPKYFPDYMITDQPERVLIAELIREKVLHYLNDEIPHGVAVEIEKMK
                     ARNDKEIVDVSAVIYCERDSHKGIIIGKNGRKLKGIGKSARQDIELLLGSQINLQLWV
                     KVKENWRNLQNYINNFGYNDK"
     gene            5465..6844
                     /gene="mgtE"
                     /locus_tag="PC700_002080"
     CDS             5465..6844
                     /gene="mgtE"
                     /locus_tag="PC700_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HDF2581391.1"
                     /translation="MDRKLDASQDLLYEVKSLIDNNKVLELRELIEEYHIIDIFDIME
                     NLEEDMKIQLFEVLPLDMASSILEEGSVEFFISILSKLDVEHSKNILELMSLGDMADK
                     LSELEEEEREHIINLLNQENADYVKELLFYDEDSAGGTMTTGYISINKDMTALEAIDH
                     MREEAEEAETIYYIYVVDDEEKLVGVLSLRELIIARDANIVEDLMSENIISVYVDEDR
                     EEAVRLVSKYNLIAIPVVDRQEKLKGIITVDDIIDVMEEEATEDMYKFAGSSEHEREV
                     AEKENPTLREQIISALRGRLPWLIITLVGGLLASLILSNLDYIMNPVYAPLVFFIPVV
                     IGMGGNIGTQSSSVTVITLSNKDLNFSNVVREGIVGIITGLLCSIITGIVIYFVMRKL
                     DIVLIVSISLFINMVLGATIGAFMPVLLKKMDADPSTVSSPIISTALDITGIAVYFII
                     TTALLSKIV"
     gene            6917..7072
                     /locus_tag="PC700_002081"
     CDS             6917..7072
                     /locus_tag="PC700_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HDF2581392.1"
                     /translation="MNNISWEVFKKTGSIDAYLYFCDCKNIHEEIEEVREKKEDDDNP
                     EHPGDST"
     gene            7038..7811
                     /gene="recO"
                     /locus_tag="PC700_002082"
     CDS             7038..7811
                     /gene="recO"
                     /locus_tag="PC700_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDF2581393.1"
                     /translation="MIILNTQGIVLKAIRYKESDIILTLFTRKLGKVSAIAKGAKKNK
                     SSLLSSSQLFSYSNFTLKKQGNMYKVTQSEIIKSFYNISYDIEAFSYATYITKLVENS
                     ILENQTNNRLFILLAQTLYLYTQDNTDNRFITAAFELKFLDYIGFKPIVNKCINCECK
                     ILQNSVFNIYEGGILCSKCSKLFDNNIKLDLTTISLMEYVLRNDILTCSKAKVSKYIT
                     HELENILDKYLKVYVDNINFKSLHVLQSVKNNKGVDNDE"
     gene            7804..8403
                     /locus_tag="PC700_002083"
     CDS             7804..8403
                     /locus_tag="PC700_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HDF2581394.1"
                     /translation="MSSNITIEMVDSVFERVPGATYAEAKEALVMCNGDVIEAVIYLE
                     NKKNTCNCKAKSAKETMEEVFGKDGENIKFQLKELLRKSSVVRIIVEKDGKTMMNIPL
                     TVGVVGLAFVPLATLVGLSAAVISKYRIKVQNEEDGEIVDLGELNEEKLHVLKNMITN
                     AAKDVKDVVVDKKEDDKDVTADLMKEESEKNKEDEDENK"
     gene            8563..9441
                     /gene="glyQ"
                     /locus_tag="PC700_002084"
     CDS             8563..9441
                     /gene="glyQ"
                     /locus_tag="PC700_002084"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDF2581395.1"
                     /translation="MNFQEMILALQKYWSKQGCIMMQPYDIEKGAGTMNPNTFLRSLG
                     PEPWQVCYVEPSRRPADGRYGENPNRLYQHHQFQVILKPSPDNIQELYLESLKEIGID
                     PSEHDIRFVEDNWEAATVGAWGLGWEVWLDGMEITQFTYFQQVGNIECELETGEITYG
                     LERLAMYIQEVDSVYDLKWNDKITYGEVFNKAEYENSMYAFELCDADMLFNLFDIYEK
                     EALRLMENGLVIPSYDYVLKCSHAFNTLDARGAIGVSQRASFIGRVRNMAKTVAETFV
                     KQREEMGFPLLKDGDK"
     gene            9441..11507
                     /locus_tag="PC700_002085"
     CDS             9441..11507
                     /locus_tag="PC700_002085"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDF2581396.1"
                     /translation="MKNYLLFEIGVEEMPSRFVGSTLEQLKNNLSKLFDENRIAFDKI
                     NAYGTPRRLTLVVEGLSDRQSDLEEEIKGPAKKIAVDAENNLTKPALGFIKSKGLSES
                     DIYFKQVGKEEYIFATIKQDGKETSEVLKDILPETIKSVVFPKAMRWGGKNLKFARPI
                     RWIVALLNDKVLEFDLEGIISSNVTKGHRFLGESTIEVDSIEDYFEKLEKNYIVLDQN
                     KRKEMIRKQCIEVANSLGGEVEFDEDLLEEVTYLVEYPTAFYGEFDVDYIKLPKEVVI
                     TPMKQHQRYFPVLKEGKLLPNFIAVRNGDSYRIDNVKAGNEKVLEARLADALFFYRED
                     TKRNLESYIEKLKTVVFQVKLGTIYDKTLRLEKLSADILDSLGLVDEKADTIRAAKLS
                     KADLVTGMVGEFDELQGFMGKEYAKVGGENDVVSEAIFEHYLPRFAGDILPKTNAGVA
                     LSIADKLDSIAGFFAIGIQPTGSQDPYALRRQALGILSILMDRKVAVDIKVLIEHALD
                     NYSELDFNKEEVVEQIMNFFNERVKNLFKDLGIRYDVVDAVLSSDINDVSDMHMRALE
                     LNNWLEKDELVEMLTAFNRVSTLAQKAESDKIDESLLKEDAEIRLYNEFKQVKVKVNE
                     LLKDKKYGIALDEFASLRPVVDNLFDTVMVMDNDEAIKNNRLALLKQVYDTMLEICDL
                     SKIVYK"
     gene            complement(11673..13220)
                     /locus_tag="PC700_002086"
     CDS             complement(11673..13220)
                     /locus_tag="PC700_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HDF2581397.1"
                     /translation="MDNFKKILLNELPNFKEYSSKFLNGEINKMQYKGFSGGYGVYAQ
                     RDKKSFMIRLRVSAGVLSQYQLNKIYEIAIKHNLDKIHLTTRQAIQLHDLSINSIVDI
                     MEEGIKNDIFTRGGGGNYPRNVGLSPLSGVDPSEVFDVTPYAVATDKYFIKNATTYHL
                     PRKLKVSYSNCYTDTAHCSIQDLGFVATLKNDEPYFRVFLGGGLGKQPKVALELDELI
                     KPKDALYCVEGMIKFFMDYGNYENKNRARVRYMVESLGEELFLEKFKEYYKLEKENGS
                     LELNIEEIDYSKPGVKIDIQDSRLIPQKQDGLYTVYIHPVGGILLTKDLSTLLKELDN
                     VENPMIRLGMTEGLYILNLNGNEAKRILEISKTISCNSQLEQSISCIGVPICQMGIQN
                     SQKMLHEIIDYFRLQNNEEILNKAPKLYISGCLNSCGVHQVGSIGLCGKKKNVDGIST
                     DAFELFVGGSFEIGKTRLGKSLGDFKASDIPEMLYKISDASSGNFYEWVNSNDNILNK
                     ITDKYKI"
BASE COUNT         5228 a         1449 c         2373 g         4208 t
ORIGIN      
        1 aaaattttaa aactaatctg ttacaacatc aataaagtgc ataaatacct atttcaagca
       61 taaaattaat taagggggta atgcttatgt tagaaatatc cactgattta caagtaaatc
      121 aaccagttat tactgttaca agtaatacgt ttattagtat agaaaattat ctatcaatat
      181 tagaatatga agttgattta ataagaataa agactaaagt taaaacaatt aaaatatctg
      241 gggataaact ttcacttaag tatattactg atagtgagat aggtataaaa gggattatct
      301 acaatgttga gtatgttgat taggaggaga tacacttgat aagttttata aggggatact
      361 atgttatagt tgtggaagga gtaggactcg aacaattttt aaatcatctt ataagaaatg
      421 ggataagtgt ttacaatgta actagaataa aaaatactaa aatggagttt catatagata
      481 gacaagatat aaaagaattt aagaatgttt atagaggaag taagtttgat ataaaggtaa
      541 agcaaaaaac tggggtccct tttattatca aaagagttta taagcacaaa ggtatgtgga
      601 tttgtgctct tgtttcttta tttttattga tgtctacatc gcagtttgtg acagatgtat
      661 atatacaatc tcctgaaggt ataaaaaaag aagctcttag aaatgagctt tataaggttg
      721 gagtaagacc aggagtctat aagaagagta ttgatagaaa agaagttaga gaccatatga
      781 tgtcaaaatt taatgatgtg gcatatttat ctatcaatgt aaaaggaaca aatatatttg
      841 ttactgttac aaaaaaggca gaatccctaa aatctacaga ccaatcaaat tattgtaatg
      901 taattgcttt aaaaaatggt ataatagaaa aggtaatccc tagaagtggt aaatcagtta
      961 ttaaatctgg agacatagtc caaaagggag atgtcttatt aaatggagct aataccaagt
     1021 caataccaga agtatgggca agtacttttt atgagtccac aaaaaaggca agttatgtag
     1081 atactgtaaa taaaaaaact ggagaaaaga aaaatatata tacactaagt ttttatgata
     1141 aagagtttac aatgagaaaa aatataaaat ataaagatta tgttgttgaa aataaagaaa
     1201 aaaaactatc aataggaaac tatacttttc caataaagat aaaaacgagt actttttatg
     1261 aaacaaaaaa ggttgaagta aaaaggaata aagaagagtt aaaaaaagaa ttatctgaaa
     1321 aagctttaaa agagttagaa tacataatcc cagcttcggc tagaataata gatgtaaaac
     1381 ataactttaa ggttaataaa aatatgttag aatatttaat aacagttcaa actagtgaaa
     1441 atattgctaa aatttatcct cttagtaagt cagaagcaga aaggtttata aaggaagaaa
     1501 gtaaaccaga tgaaggtgaa gaggaagttc cttccaatcc agaaaaaaga ccattaaatg
     1561 atataagaaa tgagtttgat gaggataata aggataataa agaccaaaac aattctgatg
     1621 aaaataatag taatcaaaat agtaataata gtcaaaataa taatagcaat aacaactaat
     1681 aaaattaaac ttaaaatctt aggaggacta attcaattga taatacaaaa gaaatttaca
     1741 atagcagatg gtaactttga aagagagtta tttggcaact ttgatgaaaa tgtaaaatta
     1801 atagagaaga ctttaaatat agatgtaata cttagagaag gcaatataat tttaattgga
     1861 gaagaaaaaa atgtagattc tgcattaaaa cttatgaatg aattacacca aactgtatca
     1921 aatggtaagc atttagataa gcagagtatt tcttattcat tatctctttt gcttgaagga
     1981 agtgaacaaa aaataaaaga acttgagggg acaatcgtca taactcaaag gggaaaagct
     2041 gttcaaccta aaactttagg tcaaaaagaa tatataaaac ttatagaaaa taatgatata
     2101 acttttggtg ttggacctgc tggaacagga aagacttatc ttgcggttgc tatggcagta
     2161 aaagctttta aaagagatga agttagtaga attatactta caagaccagc tgtggaagca
     2221 ggagagagtc taggtttttt accaggagat ttaaaggaca aagttgaccc atatttaaga
     2281 ccactatatg atgccttatt tgaaatgttg ggtgctgata aatttaataa gtatttagaa
     2341 agaggtacta ttgaggttgc accacttgca tttatgagag gtagaacact tgataattca
     2401 tttataatat tggatgaagc tcaaaatact acttctgaac aaatgaaaat gtttttaact
     2461 agacttggat ttggctctaa agctgtagtt acaggagatg taactcaaac agatttacca
     2521 caaaataaga agagtggtct aatacaagct acagagatcc ttaaaggtgt tccaggtatt
     2581 gggtctataa tgctaacaga tagagatgtt gtaagacatg agttggtaca gagaataata
     2641 agagcttatg agaagcacga caaaagagaa gaatttaaaa aagaagaacg taaaaaagtg
     2701 aaaatgcagg aaaagacttt caaaagaaag taaaagtgta gtaatataga tatatagcat
     2761 tattaatgaa taaaaagtag gtgtagtgaa tggatttgat actagatgat agacaagata
     2821 aattagaagt aagtgaggag ttaattgaaa agataaagga tataatcata gaatgtttag
     2881 attatgaagg atatgatgat aattatgaag tgagtctttc ttttgtagat aataaagaga
     2941 ttcatgaatt aaatagagaa tatagaggag ttgatagagt gactgatgtt ttatcatttc
     3001 ctcttttaag tgatgatttt gaggatgttg agttagaaga agaatcccta ggtgacatag
     3061 tagtatctct tgaaagagcc ttagaacaga gtatagagta taaccatagt tttgaaagag
     3121 aagtatgttt tttaatatgt catagtatgt ttcatctttt aggttatgac cacgatacag
     3181 atgaaaatac aaaagaaatg agagaaaaag aagaacacat attaaataag ttaaacataa
     3241 caagggagta gtttttatga aaccagaaaa aactcgtcaa ggtataatta aagcatttaa
     3301 tgcagctata gaagggatat tgtatacatt taaatttgaa agaaatatga aaatacatta
     3361 tttaggttca gttgctgttc ttataataag tttgtttttt aacttttcaa aattagaaat
     3421 gataatgtta cttatgtcca tatgcttagt tgtagttgcc gaaatgttta atacagctat
     3481 agaaaaagct gttgatttag taactgatga atatcatgtt cttgctaaga tagcaaaaga
     3541 tgttgcagct ggaggcgttc tagtggcagc actaaattca gtagtagttg gatatatttt
     3601 gttttatgat aaattgacag atatatctgg aatattaatt tataaaataa gagaatcaga
     3661 attacatata accttgatat gtatattatt ggttcttata gctgttgtag ttgtaaaagc
     3721 attgacttct acaggtactc ctctaaaagg aggaatgcca agtggtcatg ctgccttagc
     3781 atttgctata gcaactgcaa ttacacttat gactgaaaga gttgttgcct ctactcttgc
     3841 atatataatg gctgtattag ttgcccaaag tagaattgaa ggaaaaatac ataccttttg
     3901 ggagactatt gctggagctt tattaggtgt gttgatagct atattagtat ttcagcttgg
     3961 aatgttctat aattaaaata aatgtacata aaatatttaa aataaaacaa atctgtgcta
     4021 attaaatttt tatgatataa tgtagagtat taaataaaaa taacttttaa ttattgtata
     4081 caggaggagt tgttgtggat aacatagaat tgttaaggtt ggcagaagat gccagacaac
     4141 attcttatgc tccttactca ggttttagag taggtgcagc tcttttaact aagagtggta
     4201 aagtttatac tggatgtaat atagaatgtg catcattagg tggaacaaat tgtgcagaaa
     4261 gaacagctat ttttaaagca atatcagagg gtgataagga tatctataaa atagctatag
     4321 caagtgataa ctcagaaaat aatgagcaaa catatccatg tggaatatgc aggcaggtaa
     4381 ttatagagtt tggttctgat ataaaaataa taacgggata tactaaagga gaggtgtttg
     4441 agcatagtat aaaggatttg ttgccaaact atttctctgg aaatgattta aaataaattt
     4501 aaaagtataa ataaatttaa gtaattaaaa caagaagata aaaagttagg agacagttaa
     4561 tgttcaaatc aggttttgtc agtatagtgg gtagaccaaa tgtaggaaaa tctactttaa
     4621 tgaataatgt agttggagaa aaaatagcta taatgagtga taagcctcaa actacaagaa
     4681 atacaataca agcagtatat acagatgaag aaatgcagat agtattctta gatacacccg
     4741 gtattcacaa accaaaaaat aaattaggtg aatttatggt aaaggcagca acagaagctt
     4801 ttaaaaatgt agatttaata ttatttgtag ttgatgattc taaaaaaata ggtcctggag
     4861 atagaaagat aatagaggat ttaagaagtg ttaagacacc aataatttta gttgtaaaca
     4921 aaatagacca actagaccaa aaagatgagc tatttgatat aatcaaaatg tatgatagag
     4981 aaggtatttt taaagaaata gtaccaatat cagcattaaa aggaaagaat acggatacct
     5041 taataaaagt tatacaaaat tatttagaag aagggccaaa gtattttcca gattatatga
     5101 ttacagacca gccagaaaga gtattaatag cagaacttat aagagaaaaa gttcttcact
     5161 atcttaatga tgaaatacct catggtgtag ctgttgaaat tgaaaaaatg aaagcgagaa
     5221 atgataagga aatagtagat gtatctgctg taatatactg tgaaagagat tcacataagg
     5281 gaataataat aggaaaaaat ggtaggaaac taaaaggaat aggtaagtca gcaagacaag
     5341 atatagaatt gcttttaggt tcacaaataa atcttcaatt atgggttaaa gtcaaagaaa
     5401 attggagaaa tttacaaaat tatataaata attttggata taatgataaa taaaggtggg
     5461 ttatatggat aggaaactgg atgcttccca agatttatta tatgaagtaa aatcattaat
     5521 tgataataat aaagtattgg agttaagaga attaatagag gaatatcata taattgatat
     5581 attcgatata atggaaaatt tagaggaaga catgaaaatt caattatttg aagtacttcc
     5641 tttagatatg gcatcttcta tattagaaga aggtagtgta gagtttttta ttagtatttt
     5701 atctaaactg gatgtagaac actcgaaaaa tatacttgaa cttatgtctc ttggagatat
     5761 ggcagacaag ttgagtgaac ttgaagaaga agaaagagaa cacataataa atcttttgaa
     5821 tcaagagaat gccgattatg taaaagagtt actattctat gatgaagatt ctgctggtgg
     5881 aacaatgacc actggatata tttctataaa taaagatatg acagctcttg aggctataga
     5941 ccatatgaga gaagaagctg aagaagctga aactatttat tatatatatg tggttgatga
     6001 tgaagaaaaa ctagttggag tattgtcatt aagagaatta ataatagcta gagacgcaaa
     6061 tatagtagaa gatttgatga gtgaaaatat aatctctgtt tatgtagatg aagatagaga
     6121 agaagcagta agattagtat ctaaatacaa tttaatagca attccagtag tagacagaca
     6181 agaaaaatta aaaggaataa taacagtaga tgatataatt gatgtaatgg aagaagaagc
     6241 aactgaagat atgtataaat ttgcaggaag ttcagaacat gaaagagaag ttgctgaaaa
     6301 agaaaaccct acattgagag agcaaataat atcagcactt agaggacgat taccttggtt
     6361 aattattacc ttggttggag ggttgttggc ttcgttaata ctttctaact tagactatat
     6421 aatgaatcct gtatatgctc cattagtatt ttttatacct gtagtaattg gtatgggtgg
     6481 aaatattgga actcaatcat catctgttac agtaataaca ttatctaata aggatttaaa
     6541 ttttagcaat gtggttagag aaggtattgt aggtattatt acaggtcttt tgtgtagtat
     6601 aataacaggt attgtaattt actttgttat gagaaagtta gacattgtat taattgtttc
     6661 aatatcatta tttataaata tggttttagg tgcaacaata ggtgcgttta tgcctgtttt
     6721 attaaaaaaa atggatgcag acccatctac agtttcatca ccaattatat ctacagctct
     6781 tgatataact ggtatagcag tttactttat aataacaacg gcattattgt caaaaattgt
     6841 ttaataactt tatgtatgat ttatcctctt cttactaaaa atataagtat aaatgtaaga
     6901 agggggtttt ttaaaaatga acaatatcag ttgggaagtt tttaaaaaaa caggtagtat
     6961 agatgcatat ttatatttct gtgattgcaa aaacatacat gaagaaattg aagaagtaag
     7021 ggagaaaaaa gaagacgatg ataatcctga acacccaggg gatagtactt aaagctataa
     7081 ggtataagga aagtgacata atacttacac tatttacaag aaagcttgga aaagtatctg
     7141 caatagcaaa aggagctaaa aaaaataaga gctctttgct ttcatcatca caattatttt
     7201 cttatagcaa ttttacctta aaaaaacaag gtaatatgta caaggttact caaagtgaaa
     7261 taataaaaag tttttataat atatcctatg atatagaggc attttcttat gctacatata
     7321 ttacaaaatt agttgaaaat tctatacttg aaaatcagac taataatagg ctatttatat
     7381 tacttgcgca gactctgtat ttgtatacac aggataatac agacaatagg tttataacag
     7441 cagcattcga gttaaaattt ttagactata taggatttaa acctatagta aataaatgca
     7501 ttaattgtga atgtaaaatt ttacaaaatt ctgtgtttaa tatctacgaa ggtggtatac
     7561 tatgtagtaa gtgtagtaaa ctttttgata ataatataaa attagactta acaactataa
     7621 gtcttatgga atatgtatta agaaatgata ttttaacatg tagtaaagct aaagtatcaa
     7681 aatatataac acatgaactt gagaatattc tagacaagta tttgaaagtt tatgttgata
     7741 atattaattt taaatcatta cacgtattgc aaagtgtaaa aaataataag ggagtggata
     7801 atgatgagta gtaatataac tattgaaatg gttgattcag tctttgaaag agttccagga
     7861 gcaacttatg cagaagcaaa agaagcatta gtaatgtgca atggagatgt aattgaggca
     7921 gtaatatatt tagaaaataa aaaaaatact tgtaattgta aagcaaaatc tgctaaagaa
     7981 actatggaag aagtatttgg caaagatgga gaaaatataa agtttcaatt aaaagagctt
     8041 cttagaaaaa gtagtgtagt gagaataata gttgaaaaag atggaaaaac tatgatgaac
     8101 attcctttaa cagttggagt tgttggatta gcttttgtac cattagctac tttagttgga
     8161 ttatcagctg ctgttataag taaatatcgt ataaaagttc aaaatgaaga agatggagaa
     8221 attgtagatt taggtgaatt gaatgaagaa aaacttcacg ttttgaagaa tatgataact
     8281 aatgcagcaa aagatgtgaa ggatgtagta gttgacaaaa aagaagatga caaagatgta
     8341 actgctgacc ttatgaaaga agaatctgaa aagaataaag aagacgaaga cgaaaataaa
     8401 tagatataaa gtgactttta aaaaatgaag agaagataat tataagaatt atcactaaca
     8461 ttgacaaatt ttatagaatt tgatattcta aaagttgtag aagaactatt aaaatagcct
     8521 ttcgtgtaga cgagaggctt tattgttata ggagggattt ttatgaattt tcaagagatg
     8581 atactggcgt tgcaaaaata ctggtcaaag caaggttgta tcatgatgca accttatgat
     8641 attgaaaaag gtgcaggaac tatgaatcct aatacttttt taagatcatt aggacctgag
     8701 ccttggcaag tatgctatgt agagccttca agaagaccag cagatggaag atatggtgaa
     8761 aacccaaaca gactatatca acatcatcaa ttccaagtta tactaaaacc ttctccagat
     8821 aatatacaag aattatattt ggaaagttta aaagagattg gaattgaccc aagtgagcat
     8881 gacataagat ttgttgaaga taactgggaa gctgctacag ttggtgcttg gggacttgga
     8941 tgggaagttt ggctagatgg tatggaaata actcaattta cgtattttca acaagtaggt
     9001 aatattgaat gtgagcttga gacaggagaa attacatatg gattagaaag attagctatg
     9061 tacattcaag aagttgatag tgtttatgat ttaaaatgga atgataaaat aacttatggt
     9121 gaagtattta ataaagctga gtatgaaaac tcaatgtacg catttgaatt atgtgatgca
     9181 gatatgttgt ttaatctgtt tgatatatat gaaaaagaag cattaagact tatggaaaat
     9241 ggccttgtaa taccttcata tgactatgtg ttaaaatgct ctcatgcatt taatacattg
     9301 gacgcaagag gtgcaatagg agttagtcaa agagcttcat ttataggtag agttagaaat
     9361 atggcaaaga ctgttgcaga aacttttgta aaacaaagag aagaaatggg attccctctt
     9421 ttaaaggacg gtgacaaata atgaaaaatt atcttttatt tgaaatcggt gttgaagaaa
     9481 tgccatcaag atttgtaggc agtacactag aacaattaaa aaacaatctt agtaaattat
     9541 ttgatgaaaa tagaatagct tttgataaaa taaatgcgta tgggacacca agaagactta
     9601 ctttagtagt cgaaggattg agtgatagac aaagtgattt agaagaggaa ataaagggtc
     9661 ctgctaagaa aattgctgtt gatgcagaaa ataatttaac aaaaccagca ttaggattta
     9721 taaaaagcaa aggattaagc gaatctgaca tatattttaa acaagttgga aaagaagaat
     9781 atatatttgc gactataaag caagatggaa aagaaacttc agaagtacta aaagacatat
     9841 taccagagac tataaaatct gtagtattcc caaaagctat gcgttgggga ggaaaaaatc
     9901 ttaaatttgc aagacctata agatggatag ttgcattgtt aaatgataaa gtattagagt
     9961 ttgatttaga aggaataata tcttctaatg taacaaaagg tcatagattt ttaggtgaaa
    10021 gtacaataga agttgattct atagaagatt attttgaaaa attagaaaaa aattatatag
    10081 ttttagacca aaacaagaga aaagaaatga taagaaaaca atgtatagaa gtagcaaatt
    10141 ctttaggtgg agaagttgaa tttgatgaag atttacttga agaggtaact tatttagttg
    10201 aatatccaac tgctttttat ggagaatttg atgtggatta tataaaacta ccaaaagaag
    10261 ttgttataac tcctatgaaa caacatcaaa gatattttcc agtacttaaa gaaggaaaac
    10321 tgttacctaa ctttatagct gttagaaatg gtgattctta cagaatagac aatgttaaag
    10381 ctggaaatga aaaagtttta gaagcaagac ttgcagatgc tttatttttc tatagagaag
    10441 atactaagag aaacttagaa agctacatag aaaaattaaa aactgttgta ttccaagtaa
    10501 aactaggaac tatttacgac aaaactttaa gacttgaaaa attaagtgct gatatacttg
    10561 atagtttagg tttagtagat gaaaaggcag atactataag ggctgctaaa ttatctaagg
    10621 cagatttagt tactggaatg gtaggagaat ttgatgaatt acaaggtttt atgggaaaag
    10681 aatacgcaaa agttggagga gagaatgatg ttgtaagtga agctattttt gaacattatc
    10741 ttccaagatt tgcaggagat atacttccta agactaatgc aggggttgcc ttatcaatag
    10801 ctgataagtt ggattcaata gcaggtttct ttgctatagg aatacaacca acaggttctc
    10861 aagacccata tgctttaaga agacaagctt taggtatact tagtatactt atggacagaa
    10921 aagttgctgt ggatataaaa gtacttattg aacatgcttt agataattat tctgaattag
    10981 attttaataa agaagaagtt gttgagcaaa taatgaattt cttcaatgag agagtaaaaa
    11041 atctatttaa agatttagga ataagatatg atgttgttga tgctgtttta agttctgata
    11101 taaatgatgt ttctgatatg catatgagag ctttagagct taataattgg ctagaaaaag
    11161 atgaattagt ggagatgctt actgcattta atagagtttc tactctagct caaaaagcag
    11221 aaagtgataa aattgatgaa tctttattaa aagaagatgc tgaaattaga ctttacaatg
    11281 aatttaaaca agtaaaggta aaagttaatg aattattaaa ggataagaaa tatggtattg
    11341 ctttagatga atttgcatct ttaagaccag tagttgataa tttatttgat acagttatgg
    11401 ttatggataa tgatgaagct attaagaaca atagacttgc tttacttaaa caagtatatg
    11461 atactatgct agaaatttgt gatttatcta aaattgttta taaataaaat ttaaactatt
    11521 tataagaaat aaggctgtct caaaatagag atagtcttat ttttcattgt tgagatgatg
    11581 aaaatatatt tgatttcata gaaaaagagt gtctcataaa ctaaaaagtt cattttgaga
    11641 cacccatgct ttttgtaagt aaatataata aattatattt tatacttatc agttattttg
    11701 tttaaaatat tatcatttga atttacccat tcataaaaat tgcctgatga tgcgtccgat
    11761 attttataaa gcatttcagg aatatcacta gctttaaaat ctccaagtga ttttcccaat
    11821 ctagtcttac ctatttcaaa actgcctcca acaaataatt caaatgcatc tgtagatata
    11881 ccatcaacat tcttcttttt accacataaa cctatgctac caacttgatg aacaccacat
    11941 gaatttaaac atcctgatat atataactta ggtgctttat ttaaaatttc ttcattattt
    12001 tgtaatctaa aataatctat gatttcatgt aacattttct gactattttg tatgcccatt
    12061 tgacaaattg gaacacctat gcaagatata ctttgttcta attgagaatt acaactaata
    12121 gttttactta tttctaatat tctttttgct tcatttccat ttaaatttaa aatatataat
    12181 ccctctgtca ttcctagtct aatcatagga ttttcaacat tatctaattc tttaagtaaa
    12241 gtacttaaat cctttgttaa caaaatacct ccaactggat gtatatatac agtgtataaa
    12301 ccatcctgtt tttgaggtat tagtctagaa tcttgtatat ctattttaac tccaggttta
    12361 gagtaatcta tttcttcaat attgagttca agactaccat tttctttttc taatttatag
    12421 tattctttaa atttttctaa gaatagttct tcaccaagtg attcgaccat atatctaacc
    12481 ctagctctgt ttttattttc ataatttcca taatccataa agaatttaat cataccttca
    12541 acacaatata aggcgtcttt cggctttata agttcatcaa gttctaaagc aactttgggt
    12601 tgtttaccta gacctccacc taagaaaact ctgaaatatg gttcatcatt tttcaaagta
    12661 gcaacaaatc ctaagtcttg tatactacaa tgagcagtat ctgtgtaaca attagaataa
    12721 gaaactttta attttctagg taaatggtat gtagtagcat ttttgataaa atatttatca
    12781 gtagcaactg catatggagt aacatcaaag acttcactag ggtcaactcc agataatgga
    12841 gataatccga catttctagg ataattacca ccaccacctc tagtgaatat atcatttttt
    12901 attccttctt ccatgatatc aacaattgaa tttatagata aatcgtgtaa ttgtatagct
    12961 tgtctagttg ttaagtgtat cttatctaag ttgtgtttaa tagctatttc atagatttta
    13021 ttaagttgat actgagataa aactccagca gatactctaa gtcttatcat aaaagatttt
    13081 ttgtctcttt gagcataaac accatatcca ccagaaaatc ccttatattg cattttattt
    13141 atttctccat ttaaaaattt tgaagaatat tctttaaaat taggtagttc atttaacaat
    13201 atctttttaa aattatccat tatttcacct cattgttagt cttgtataaa aatatatc
//
LOCUS       DAMJWP010000087        13236 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.091, whole genome shotgun sequence.
ACCESSION   DAMJWP010000087 DAMJWP010000000
VERSION     DAMJWP010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13236
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.091"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(65..2065)
                     /locus_tag="PC700_002087"
     CDS             complement(65..2065)
                     /locus_tag="PC700_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2581398.1"
                     /translation="MKQSIAIVTDKITKLSSFLEENIRLVLGNYIDINHYYLEVLKEN
                     DKIQGDFVLVMNDDRLNSMRKHLVQDSKIIVIRRTLKENEIYGLFSIPNGTEVLVVND
                     TKETTLETISLFYKIGVKNLKFTPYIEGKIYKNIDIAITPGVKEKVPSYINKVIDLGN
                     RYIDISTFIEIINGLRIDVKEIRKNLMAYSESLISLDNGVKDNYRQLFLKIEEQDVIL
                     NLSKDGIIFTSADGIINTFNKEALKILGINENPQGKNIEDIISEDLNILLEDKEIIDE
                     VVFTNKKYINVNKKSIFSMGNKAGIYYNLQEITYIKKLEQNLTNKLREQGQIARYTFK
                     DIKTKNKNMVKCVELAKKISKSDLSVLIIGESGTGKELLSQSIHNASNRSNQPFIAVN
                     CAAVPDSLLESQLFGYEKGSFTGALREGKKGLFELANNGTIFLDEIGDMPPLLQTKLL
                     RVLQEKQVMPIGSHNVINIDVRVIAATNKNLLKMIREGKFREDLYYRLNVLPIDVPSL
                     RNRKEDIITLMNFFLKNNVRLSPEVITILEKYNWPGNIRELQNVASYVSLMCDSMVFR
                     DDLPPYIRVECANNYEDGFGYTKKEFEEILRILYERRESSSGVGRGFIIEKLIQEGIE
                     ISESKVRKVLSYLSECGYISSKSGRGGSQITSEGIKLYNNIN"
     gene            complement(2096..3814)
                     /locus_tag="PC700_002088"
     CDS             complement(2096..3814)
                     /locus_tag="PC700_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2581399.1"
                     /translation="MNFRLPKKRLPKKIIFICIILISIILFIKYDQNGLKEREIINKG
                     KYMSESLVADKEYDEAKKVVKTSFESIPEKKYNKVYNEVWDMLKTISYVPDGTKIVID
                     SLEKMRESDVKLSDECRFNIEKRLASVYIMDNNYSKAVDLTVKSIKLAEKLGNNYEKA
                     RLDVDLASIFLNIGSYETGEKLIKDSFKIDIDEGEKNGMVRLYAIINLAEIYVEEGEY
                     DKAIEISSRIKDYKKFVNTDNYNDFDIMASIMKSNAYVGKNNIQKAKTFLEKADYLIK
                     SDRTVYILDKDMYYYMAMGNLEYKSENYNSAIDNYKKSLEISTKRKLTQEKIKNLNKI
                     LNIYEKQGNIESLNEYQKMLINEYKENKSIRDSETSFYIIDKVSNESKLFEKTKKEIK
                     YYKILFTVILIAILGSTFLYNRLKYFKAQNLHDGLTNVYNRKSFDIMYEKYREKDDNF
                     ALVMIDIDNFKLINDNYGHKFGDTVIKGITNTICVMLEQDDKVFRYGGEEFSVLIRNK
                     SGEEVADIIDNIRVAIANKKWNDDVTVTISAGVAHISDSKDVLEEADKNLYKAKQLGR
                     NKVVYK"
     gene            complement(4040..5278)
                     /gene="fabF"
                     /locus_tag="PC700_002089"
     CDS             complement(4040..5278)
                     /gene="fabF"
                     /locus_tag="PC700_002089"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDF2581400.1"
                     /translation="MNKRVVITGLGCVTPLGTGKEEFWSNIKSGVSGIDKITNFDAST
                     YQTQIAGEVKNFHPEEYISKKELKRLDKFAQFAIVSAKLAVEDANLDLDKVDRERFGV
                     IIGSGIGGVEAIETQHKILLEKGNKRVSSLFVPMMIGNMAAGQVSIFLGAKGPNTNVC
                     TACASGTHSIGDAFKVIQRGDADIMVAGGSEAAVTGLAFAGFCNMKAMSTRNDDPKTA
                     SRPFDKDRDGFVMGEGAGIVILEDLEHALARGAKIYAEVVGYGLTADAYHMTTPAENG
                     EGAARSMNMALKDGNVPLEEVDYINAHGTSTYYNDLYETMAIKTVFGEKAYDLCVSST
                     KSMTGHLLGASGAIEAVVCAMSIEDSFVPPTINIQEVGEDLDLDYVPNQGKEKNIRYA
                     LSNSLGFGGHNATIVLKKYV"
     gene            complement(5307..5531)
                     /gene="acpP"
                     /locus_tag="PC700_002090"
     CDS             complement(5307..5531)
                     /gene="acpP"
                     /locus_tag="PC700_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDF2581401.1"
                     /translation="MFNKVVEIIIEQLGVEDKEITMETSLMKDLEADSLDAVEIIMAL
                     EDEFGIEIPDTEAENFKSIGDIVKYIEANK"
     gene            complement(5585..6334)
                     /gene="fabG"
                     /locus_tag="PC700_002091"
     CDS             complement(5585..6334)
                     /gene="fabG"
                     /locus_tag="PC700_002091"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HDF2581402.1"
                     /translation="MINLTGQVAVVTGGSRGIGKEIAKKLASFGADVVINYTSKEDEA
                     LKTKNEIESMGVKCTSIKCDVSKFDEVNQMIDSVVSEFGKIDILVNNAGITKDGLLMR
                     MKEEDFDRVIDINLKGVFNCTKAVTKPMMKKKYGRIINMTSVVGIMGNAGQTNYCASK
                     AGVIGFTKASARELASRNININAVAPGFIETDMTRVLSDDVKESTLANIPKKSYGKPE
                     DVANAVAFLVSDMSSYITGQVINVDGGMVMQ"
     gene            complement(6334..7284)
                     /gene="fabD"
                     /locus_tag="PC700_002092"
     CDS             complement(6334..7284)
                     /gene="fabD"
                     /locus_tag="PC700_002092"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDF2581403.1"
                     /translation="MGKVALVFPGQGAQYVGMAKDLYENNAVAKSVIDEASDALNMDL
                     KNLMFNGNEEELSKTENTQPAMVTHSVAVLKAVQAQIDLKYNACLGLSLGEYSALVAA
                     DAIDFKDAVCLVKKRGKFMQETVPAGVGAMAAILGLDRNVLENVVKDIQGGIVEVANY
                     NSPGQIVISGENEKIEEAMVKCKEAGAKRAVKLNVSGPFHSSMLKEAGVKLASELEKV
                     NITKPKVDVVANVNADYYKNEGKDLLIKQVSSSVLWEDSIEILLNDGYDTFIEMGPGK
                     TLKAFIKKIASNKKANVNIYNIDGIDSLNEFVQNYRNGEI"
     gene            complement(7300..8229)
                     /gene="fabK"
                     /locus_tag="PC700_002093"
     CDS             complement(7300..8229)
                     /gene="fabK"
                     /locus_tag="PC700_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HDF2581404.1"
                     /translation="MNKICKILNIKYPVIQGGMAWVATASLASAVSNAGGLGIIAAGN
                     APKEAIKKEIVECKKLTDKPFGVNVMLMSPFVDDIIDLIIEEKVQVITTGAGNPAKYM
                     DRLKEAGTKVIPVVPTIALAQRMEKLGATAVIAEGTEGGGHIGELTTMVLVPQVADAV
                     NIPVIAAGGIVDGRGIAASFALGASAVQVGTRFICSEECSVHSNYKNLVLKAKDRDAI
                     VTGRSTGHPVRTLKNKLSKEFLKMEQNGATPEELDKKGTGALRFATVDGDIEKGSFMA
                     GQSAAMVKEITPCKEIIEAMVNQAREIMPAIEL"
     gene            complement(8338..9324)
                     /locus_tag="PC700_002094"
     CDS             complement(8338..9324)
                     /locus_tag="PC700_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDF2581405.1"
                     /translation="MNTKAGILGVGSYLPEQSYDNFHFEKIMDTSDEWISTRTGIKER
                     RFAKESEATSDLASKAALKAIECAKLNVEDIELIILATITPDMSLPSTACIVQDAIGA
                     VNATAFDISAACSGFVYGVTIAKQFVETGCYKNVLVIGAETCSKFLNYDDRTTAVLFG
                     DGAGAAVIGPVNEGGILSTHMGSDGKGKDCLKVPAGGSRLKASKETVEANLHTIEMAG
                     SDVFKFAVRKMAETSLRALEKANLNTTDIDYLVPHQANIRIIQASSKRLELDMKKVYV
                     NIDKYGNMSAASIPVALDEAYREGKIKKGDNVVLVGFGGGLTWGASVVKWTL"
     gene            complement(9350..10372)
                     /gene="plsX"
                     /locus_tag="PC700_002095"
     CDS             complement(9350..10372)
                     /gene="plsX"
                     /locus_tag="PC700_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDF2581406.1"
                     /translation="MKIVIDGMGGDNAPKSNVEGAVNAIKEYQVDLIITGDKDLLEKE
                     FSNYEFDRNKLEIVHTTEIIENEDKPVKAIRSKKDSSMVVALNLVKEGKADAIISAGN
                     TGALLAGGLFVVGRIKGIDRPCLCSAIPNVKRGMTLIADCGANADCKPKNLVEFAAMS
                     NIYSRKVLGLENPKVALANVGLEEGKGNDLVKRSYEEIKKLDLNFIGNVEAREVINAY
                     TDIIICDGFTGNILLKSAEGVALSVMSLIKETFMASTKSKIGALLIKDDLRKLKSFID
                     YSEYGGAPLLGLNGGVIKAHGSSDAKAIKNAINQGIKFSKGKVVEDINQFISKYNEEN
                     KNNEDE"
     gene            complement(10383..10940)
                     /gene="fapR"
                     /locus_tag="PC700_002096"
     CDS             complement(10383..10940)
                     /gene="fapR"
                     /locus_tag="PC700_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HDF2581407.1"
                     /translation="MKKKSKAQRQKELIDMLKTDPFYTDEELSSLFDVSIQTIRLDRM
                     SLNIPELRERVKSIAETQSSKVKTLGVKEITGEIIDLSVGRLGISMLEVTQDMIYSKT
                     NTLKDTYIFSLADSLAMAIIDAPKVIMRVANVKSFKLIEQQDRLIAKAEVYRNIDKKH
                     YVKVVINNKAQEQIFRGKFIFEELD"
     gene            complement(11147..11323)
                     /gene="rpmF"
                     /locus_tag="PC700_002097"
     CDS             complement(11147..11323)
                     /gene="rpmF"
                     /locus_tag="PC700_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDF2581408.1"
                     /translation="MAVPKRKTSKSNTKMRRAANSKMEATGFVSCPQCHEPKLPHRVC
                     PDCGYYKGKEVVSK"
     gene            complement(11348..11872)
                     /locus_tag="PC700_002098"
     CDS             complement(11348..11872)
                     /locus_tag="PC700_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HDF2581409.1"
                     /translation="MKVSIEKINRKETDKIDLNFCEKIDTISYCDEIYKLVSPVNLKG
                     KVSKTNKGLYLDIDVNFTIVDTCSRCLKEVEIPLEYSIQGFLVKEEDYDEDEFEEFDP
                     FIFDGEEIDLIDIIEQTLDFNVPHKVLCSENCKGLCQVCGANLNEEECSCSEITNDEE
                     YIDPRFAKLKDLFN"
     gene            complement(11965..13161)
                     /locus_tag="PC700_002099"
     CDS             complement(11965..13161)
                     /locus_tag="PC700_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDF2581410.1"
                     /translation="MKILVLNCGSSSLKYQLIDMNNEEVLCIGLVERIGIEGSILKHE
                     KAGRDDKYVVEQPMKDHKDAIALVLEAVAHPEFGAVKEMKEIDAVGHRVVHAGEKFAT
                     SVVITPEVEEALKECIDLAPLHNPANIMGIDACKAILPDVPMVGVFDTAFHQTMPKSS
                     YLYGLPHELYTKYGVRRYGFHGTSHNYVSQRAAEILGKDIKDLKIVTCHLGNGASIAA
                     VDGGKCVDTSMGFTPLEGLIMGTRCGDIDPAILPFLMRKEGLDADGLDKLMNKESGVY
                     GMTGISSDFRDIEDAAKNGDERAQATLEAYVKKVQKYIGAYAAEMNGLDVVVFTAGVG
                     ENGKAIRADIASNMEFLGMKLDKEANDVRGKETVISTADSKVKMLLIPTNEELMIARD
                     TLRLVK"
BASE COUNT         3899 a         2613 c         1374 g         5350 t
ORIGIN      
        1 tataaaagtt tattatttaa atatctaaat tcaaaatatg tgatttcaat atcaaatact
       61 tagcttaatt tatattatta taaagtttta tcccctctga ggttatttga cttcctcctc
      121 taccagattt agatgatata tatccacatt cacttaaata agatagcacc tttctaactt
      181 tgctttcaga aatctcaatc ccttcctgta taagcttttc gattataaac cctcttccaa
      241 ctccagagga tgactctctt ctttcatata gaatcctcag tatttcctca aattcttttt
      301 ttgtataacc aaatccatct tcataattgt tagcacattc tacccttata tatggtggta
      361 agtcatctct aaaaaccata ctatcacaca ttagacttac ataagaagca acattttgta
      421 actctcttat atttcctggc caattatatt tttctaaaat agttataact tctggcgaaa
      481 gtcttacatt gtttttcaaa aagaaattca taagtgttat tatatcttct tttctattcc
      541 tcaatgatgg tacatctatt ggcaatacat ttagtctata atacaggtcc tctctaaatt
      601 ttccttctct aatcatcttt agtaagtttt tgtttgttgc tgctatgacc ctaacatcta
      661 tgtttataac attatgggaa cctataggca taacctgctt ttcttgtaat acccttagta
      721 actttgtctg tagcaatgga ggcatatctc caatttcatc cagaaatata gttccattat
      781 tagcaagttc aaatagaccc ttttttcctt ctcttaaagc tcctgtaaaa gagccttttt
      841 catatccaaa cagttgactt tctaatagac tatctggaac tgctgcacaa ttaaccgcta
      901 taaatggttg attacttcta ttggaagcat tatgaattga ttgtgataat aattctttac
      961 cagtaccact ttctcctatt attaatacag aaaggtctga tttagatatt tttttagcca
     1021 attctacaca ctttaccata tttttatttt tagtttttat atctttaaat gtatatcttg
     1081 ctatctgacc ttgttctctt aatttattag taagattttg ttctaatttt tttatatacg
     1141 ttatttcttg taagttataa tatatcccag ctttattccc catagaaaaa atactttttt
     1201 tatttacatt tatatatttt ttatttgtaa ataccacttc atcaattatt tctttgtctt
     1261 caagtaaaat atttaaatct tcagaaatga tatcttctat gtttttgcct tgtggatttt
     1321 catttatacc taaaattttt aaagcttctt tattaaatgt gtttattata ccatctgctg
     1381 aagtaaatat tattccatct tttgatagat tcaatataac atcttgttct tctattttta
     1441 aaaacagttg cctataatta tctttaactc cattatctaa acttattaaa ctctcagaat
     1501 atgccattaa atttttacgt atttctttaa catcaattct aagaccattt attatttcta
     1561 taaaagtaga tatatcaata tatctattac caaggtcgat tactttattt atataacttg
     1621 gtactttttc ctttactcca ggcgttatag ctatgtctat atttttataa atttttcctt
     1681 caatatatgg agtaaacttt aaatttttta ctcctatttt gtaaaataag cttatcgttt
     1741 ctaaggtagt ttcttttgta tcatttacaa ccaatacttc agttccattt ggtattgaaa
     1801 ataatccata tatttcattt tctttaagtg ttctccttat cactataatc ttactatctt
     1861 gtactaaatg ctttctcata ctgtttaatc tatcatcatt cattactagt acaaaatcac
     1921 cttgtatttt atcattctct tttaaaactt ccagataata gtggtttata tcaatataat
     1981 tacctaaaac caaacgtata ttttcttcta agaaagatga tagtttagtt attttatcag
     2041 ttactatagc tattgattgt ttcatattta tacctcttat ttatatagta tataattact
     2101 tatatactac tttatttcta ccaagttgtt ttgctttata taaattttta tcagcttctt
     2161 ctagtacatc tttgctatct gaaatatgag ctacacctgc acttatagtc acagtcacat
     2221 catcattcca ctttttattt gcaattgcaa cccttatatt gtcaattata tctgctactt
     2281 cctcaccaga tttatttctt attaatacag aaaattcttc tcctccgtac ctaaagactt
     2341 tatcatcttg ttctaacata acacaaatgg tatttgttat tccctttata acagtgtcac
     2401 caaatttatg tccataatta tcatttatga gcttgaaatt atcaatatct atcatcacaa
     2461 gtgcaaaatt atcatctttc tctctgtact tttcatacat aatatcaaaa ctttttctat
     2521 tataaacatt tgtaagacca tcatgtaaat tttgtgcttt aaaatacttc aatcggttgt
     2581 ataaaaaagt agaccctaat atagcaatta aaattactgt aaataatatt ttataatatt
     2641 ttatttcttt tttagttttt tcaaatagct tactttcgtt tgatacttta tcaattatat
     2701 agaaactagt ctctgaatct cttatacttt tgttctcttt atattcattt attaacatct
     2761 tttgatactc atttaaagat tctatatttc cttgtttttc atatatattt aatatcttat
     2821 ttaaattttt tattttctcc tgagttaact ttcttttggt agatatctct aaactttttt
     2881 tataattatc tattgcagaa ttataatttt cgcttttata ttctaagttt cccattgcca
     2941 tataatagta catatcttta tctaatatat aaactgttct atctgatttt ataagataat
     3001 cagctttttc taaaaaagtc tttgcctttt gtatattatt tttgcccaca tacgcatttg
     3061 acttcattat agaggccatt atatcaaaat cattgtaatt atccgtattt acaaactttt
     3121 tataatcttt tatcctgcta cttatttcaa tagctttatc atattctccc tcttctacat
     3181 atatttctgc tagatttata atagcatata atctaaccat tccatttttt tcaccttcat
     3241 caatatctat tttaaaagaa tcctttatta atttctctcc tgtttcatag ctacctatat
     3301 ttaaaaatat gctcgccaaa tcaacatcta gtcttgcttt ttcataatta ttccccaatt
     3361 tctcagccaa ttttatagac tttacagtta aatctactgc cttagaataa ttattatcca
     3421 ttatatacac tgatgctaat cttttttcta tattaaatct acattcatca gataatttca
     3481 catctgattc tctcatcttt tctaaagaat caatgactat cttcgttcca tctggaacat
     3541 atgaaatagt ctttaacata tcccaaactt cattatatac tttattgtat tttttttctg
     3601 gaatagattc gaaagaagtc ttaaccactt tttttgcttc atcatactcc ttatctgcca
     3661 caaggctttc agacatatat tttcccttat ttataatttc tctttctttc aatccatttt
     3721 ggtcatactt tataaacaaa ataatactaa taagtattat acatataaaa attatctttt
     3781 tgggtaacct tttctttggt agtctaaaat tcaaaatttt agtcctcctt ttgtatattg
     3841 tagaatgaaa acttgcatat ataaataacc tcttaataat taataataca atatatcaaa
     3901 atgatttaca acatatgcta gacttaaatc ttaataaaaa agtctcctaa aataatttag
     3961 gagactttaa atttaagcta atataaattt atacagttaa aacaagtgct tgttttttag
     4021 ttttatattt aatatttatt tatacatatt tttttagtac tattgtagca ttatgcccac
     4081 caaatcctaa agaatttgat agtgcatatc taatattttt ttctttacct tgatttggaa
     4141 cataatctaa atccaaatct tcaccaactt cttgtatgtt tatagttgga ggcacaaaac
     4201 tatcttctat actcatagca caaacaacag cctctatagc acctgatgct cctaataagt
     4261 gaccagtcat agattttgtt gatgacacac ataagtcata tgccttttct ccaaatacag
     4321 tttttatagc catagtttca tataaatcat tgtaataagt tgaagttcca tgagcattta
     4381 tataatctac ttcttctaat ggaacatttc catctttcaa agccatattc atagacctag
     4441 ctgctccctc tccattttca gctggagttg tcatatggta tgcatcagca gttaaaccat
     4501 atccaactac ttctgcatat attttagccc ctcttgctaa agcatgttct aagtcttcta
     4561 agataacaat acctgctcct tctcccatta caaaaccatc tctgtcttta tcaaacggtc
     4621 ttgatgcagt tttagggtca tcatttctag ttgacatagc cttcatgtta cagaatccag
     4681 caaaagcaag acctgttaca gcagcttcag agccccctgc taccatgatg tctgcatcac
     4741 ctctttgaat taccttaaaa gcatcaccta ttgaatgagt tcctgatgca caagctgtac
     4801 atacattagt gttaggccct tttgctccta aaaatattga aacttgacca gctgccatat
     4861 ttcctatcat cataggtaca aaaagtgaac ttactctttt atttcctttt tccaaaagaa
     4921 ttttatgttg agtttctata gcttcaactc cacctattcc agaacctatt ataacaccaa
     4981 aacgttctct atcaacttta tctaaatcaa gatttgcatc ttcaactgct aatttagcag
     5041 atactattgc aaattgagca aatttatcta atctcttcaa ttctttttta cttatatatt
     5101 cttctggatg aaaattctta acttctcctg ctatctgagt ttgatatgtg ctagcatcaa
     5161 aattagttat tttatctata ccagatactc cagattttat attactccag aactcttctt
     5221 ttcccgttcc caacggcgta acacatccaa gtcctgttat tactactctt ttattcatcg
     5281 gatacctcca cttttttaaa gctcgactat ttattagctt ctatatactt aactatatct
     5341 cctatagatt tgaagttttc tgcttctgta tctggtattt ctataccaaa ttcatcttca
     5401 agtgccatta ttatttctac tgcatctaat gaatctgctt ctaaatcctt cattaatgat
     5461 gtctccatag ttatttcttt atcttctact cctaattgct ctattattat ttctacaact
     5521 ttattaaaca ttgtgtacct cctaaaattt tttattttaa tagatttctc tactttttaa
     5581 attattattg cattaccatt ccaccatcta catttattac ttgtcctgtt atataacttg
     5641 acatgtcact aactaaaaat gctacggcat tggctacatc ttctggttta ccataagatt
     5701 tctttggtat gtttgctagt gttgattctt ttacatcatc acttagtact ctagtcatat
     5761 ctgtttctat aaatccaggt gctactgcat ttatatttat gtttcttgat gctaactctc
     5821 ttgcagaagc ttttgtaaat ccaattacac ctgcttttga tgcacaataa ttagtttgcc
     5881 ctgcattacc cataattcca actactgaag tcatatttat tattcttcca tacttctttt
     5941 tcatcatagg tttagtaact gcttttgtac aattaaagac accttttaag tttatatcta
     6001 taactctatc aaaatcttct tctttcattc tcataagcag accatcttta gttatgcctg
     6061 cattattaac caatatatca atttttccaa attcgcttac aacagaatct atcatttgat
     6121 tcacttcatc aaatttagac acatcacatt ttatagaggt acactttacc cccatacttt
     6181 ctatttcatt tttagttttt agtgcttcat cttctttaga agtataattg attactacat
     6241 cagctccaaa agatgctagt ttttttgcta tttcttttcc tataccccta gagccaccag
     6301 taacaactgc tacttgtcct gtaagattta tcattatatc tctccatttc tataattttg
     6361 tacaaattca ttaagagaat ctataccatc tatattataa atattgacat ttgctttttt
     6421 attggaagct atcttcttaa taaatgcttt taaagttttt ccaggtccca tttctataaa
     6481 agtatcatat ccatcattta aaagtatttc tatagaatct tcccaaagaa cagatgaact
     6541 tacttgtttt ataagcaggt ctttaccttc gtttttatag taatctgcat ttacatttgc
     6601 caccacatca actttaggtt tagttatgtt aactttttct aattcactgg ccaattttac
     6661 accagcttct tttaacattg atgagtgaaa aggtccactt acatttaatt taactgccct
     6721 ttttgctcct gcttctttgc attttaccat tgcttcttct atcttttcat tttcaccaga
     6781 tataactatt tgtccaggag aattgtaatt tgctacttct acaatacctc cttgtatatc
     6841 tttaaccaca ttttcaagta catttctatc taaacctaaa attgctgcca tagctccaac
     6901 acctgctgga actgtttcct gcataaactt acctcttttt ttaactaaac atacagcatc
     6961 tttaaagtca attgcatctg ctgcaaccaa cgcactatac tctcctaagg ataatcctaa
     7021 gcatgcatta tattttaaat ctatttgagc ttgtaccgct tttaatactg ctacactatg
     7081 tgtaaccata gcaggttgag tgttttcagt tttagaaagt tcttcttcat ttccattaaa
     7141 cataaggttt tttaaatcca tatttaaggc atcactagcc tcatctatta cacttttagc
     7201 aacagcatta ttttcatata aatccttggc catacctaca tattgagctc cttgaccagg
     7261 gaatactagt gcaacttttc ccatcttgct cctcctgatt tacagttcta ttgctggcat
     7321 aatctctctt gcttgattta ccatagcctc tataatttcc ttacaaggtg ttatttcttt
     7381 taccatagca gcactttgac ctgccataaa tgaacctttt tctatgtctc catctactgt
     7441 tgcaaatctt aaagctcctg tacctttttt atccaattct tcaggagtag ctccattttg
     7501 ttccatcttt aaaaattctt ttgatagttt attttttaat gttcttactg gatgaccagt
     7561 acttcttcct gttacaattg catctctatc ttttgctttt agtactaagt ttttatagtt
     7621 tgaatgcaca gaacactctt cactgcaaat aaatctagtt cctacttgaa ctgcactggc
     7681 acctaatgca aatgatgctg caattcctct accatctaca attcctccag cagctattac
     7741 aggtatgttt acagcatcag caacttgtgg aactaagacc atagtagtaa gttctcctat
     7801 atgtcctcca ccttcagtac cttctgctat tactgctgta gctcctaact tttccattct
     7861 ttgtgccaaa gctattgtag gtactacagg aataaccttt gttccagctt cctttaatct
     7921 atccatatat tttgcaggat ttccagcacc agtagtaata acttgaactt tttcttctat
     7981 aatcaaatca attatatcat caacaaatgg cgacataagc attacattta ctccaaaagg
     8041 tttatctgtt aattttttac actcaacaat ttctttcttt atagcttctt ttggtgcgtt
     8101 tcctgctgct attatgccaa gtcctcctgc attagataca gcacttgcta atgatgcagt
     8161 agctacccat gccattcccc cttggataac tgggtatttt atatttaata ttttgcaaat
     8221 tttattcata caattctcct tcacttgaaa tcataaaagt aaacttttta ttattaatca
     8281 actttatttt gtgtataagt ttaattttgt taattaaatc attttagata ttttatacta
     8341 taaagtccac ttgacaactg aagcccccca agttagacct ccaccaaagc caactaaaac
     8401 tacattatca ccctttttaa ttttaccttc tctataagct tcatctaatg ctactggtat
     8461 agatgcagca gacatatttc catatttgtc tatatttaca taaacttttt tcatatcaag
     8521 ctctaatctc ttagaagaag cttgtatgat tcttatatta gcttgatgtg gcactaagta
     8581 gtctatatca gttgtattta ggtttgcttt ttcaagagct cttaaagaag tttctgccat
     8641 ctttctaact gcaaatttaa acacatcact acctgccatt tctattgtat gtagattagc
     8701 ttctacagtc tctttacttg ccttaagtct tgaacctcca gcaggtactt ttaaacaatc
     8761 ttttccttta ccatcagagc ccatatgtgt agacagtatt ccaccctcat ttacaggacc
     8821 aataacagct gctccagctc catctccaaa caatactgct gttgttctat catcataatt
     8881 taaaaactta gaacaagttt ctgctcctat tacaagcaca tttttatagc aacctgtttc
     8941 cacaaattgc tttgcaatag ttactccata tacaaatcca gaacaagctg ctgatatatc
     9001 aaatgcagta gcattcactg ctcctattgc atcttgtact atacatgcag ttgaaggcaa
     9061 agacatatca ggagtaattg tagctaatat aatcagctct atatcctcta cgtttaactt
     9121 tgcacattct atagctttta aggcagcttt agatgctaag tcagaagttg cttcagactc
     9181 tttggcaaat cttctttcct taataccagt tctagtactt atccactcat ctgatgtatc
     9241 cattattttt tcgaagtgaa aattatcata tgattgctca ggtagataac ttcctactcc
     9301 taatattcca gcctttgtat tcattatata ttaaacctcc aaaacgagtt tattcatctt
     9361 cgttattttt attttcttca ttgtatttgc ttataaattg gttaatatct tcaacgactt
     9421 ttccctttga aaattttatt ccttgattta tagcattttt tatagcttta gcatcagaac
     9481 taccatgagc ttttataact cctccattta gacctaaaag tggagctccc ccatactcag
     9541 aataatctat aaaacttttt aattttctta aatcatcctt tataagtaat gctcctattt
     9601 tactttttgt actggccata aaagtttcct ttataagact cataactgaa agtgctacac
     9661 cttcagcaga tttaagtaat atattacctg taaatccatc acatatgatt atatctgtgt
     9721 atgcatttat aacttctcta gcttctacat tccctataaa atttaaatct aattttttaa
     9781 tttcttcata tgaccttttg actaaatcat tgcctttgcc ttcttctagt ccaacatttg
     9841 ctaatgctac cttaggattt tcaagaccta aaacttttct tgaataaata ttactcatag
     9901 ctgcaaattc aactagattc ttaggtttac aatctgcatt tgcgccacag tctgctataa
     9961 gtgtcattcc tcgctttaca tttggtattg ctgaacataa gcaaggtcta tcgattcctt
    10021 ttattctacc aacaacaaat aatccaccag caagtaaggc tcctgtattt ccagctgata
    10081 ttatagcatc tgcctttcct tcttttacaa gatttaatgc tactaccata gatgaatctt
    10141 ttttacttct tatagccttt actggtttat cttcgttttc tataatctca gtagtatgaa
    10201 ctatctctaa tttatttcta tcaaattcat aatttgaaaa ttccttttca agtaaatctt
    10261 tatctcctgt gattattaaa tcaacctgat attcttttat agcatttact gcgccttcta
    10321 cattagattt aggtgcatta tctccaccca ttccatcaat tactattttc ataatttccc
    10381 tcctaatcta attcttcaaa gataaacttt cctctaaata tttgctcttg ggctttattg
    10441 ttgataacaa cttttacata atgcttttta tctatatttc tatacacttc tgcttttgct
    10501 ataagtctat cttgttgttc tatcaactta aaacttttta catttgcaac tctcataata
    10561 accttaggtg catctattat agccattgcc aatgaatctg ctagagaaaa aatatacgta
    10621 tcttttaaag tattagtctt agagtaaatc atgtcctgtg taacttctaa catagatata
    10681 cctaatcgcc ctacagataa atcaatgatt tctcctgtaa tttcctttac tcctaaagtc
    10741 tttacttttg aactctgagt ttctgctatt gatttgactc tttctcttag ctctggtatg
    10801 tttagactca ttctatctaa tcttatggtc tgtatactta catcaaacag acttgataat
    10861 tcttcatcag tataaaaagg atctgtcttt agcatatcta taagctcttt ttgtctttgg
    10921 gctttacttt tctttttcat gttccctcca aattttatag ctgtaattag taccagctac
    10981 taatagtagt atatagtaac acacaaaaat tttcaatact tatattatta tttttctatt
    11041 ttttaatatg cataaaatcc acttattttc tctagtaaat catcttattt ttttatatgt
    11101 acaaaaaaaa catgtagagt atactctaca tgctttttag aagctattac ttagacacaa
    11161 cttctttacc tttataatat ccacagtctg gacacactct atgtggtaat tttggctcat
    11221 gacattgtgg gcaacttaca aatcctgttg cttccatctt tgagttagct gctcttctca
    11281 tttttgtgtt tgatttagac gttttacgct ttggtactgc catttacgcc acctccttag
    11341 tcaaaattta attaaataaa tcttttaatt tagcaaaacg aggatctatg tattcctcat
    11401 cgtttgtaat ttcactgcaa gaacactctt cttcatttaa gtttgccccg catacttgac
    11461 aaagaccttt acagttttca ctacaaagaa ctttatgagg tacattaaaa tctaatgtct
    11521 gttctattat atcaataagg tcgatttctt caccatcgaa tataaaagga tcaaattcct
    11581 caaactcatc ttcatcataa tcttcttctt ttactaaaaa accttgtata gaatactcta
    11641 gtggtatttc tacttcttta agacatcttg aacaagtgtc aacaattgta aaattgacat
    11701 ctatatctag atacaaacct ttattagtct ttgacacttt cccttttaaa tttacaggtg
    11761 atactaattt gtaaatctca tcacaataac taatagtatc aattttttca caaaaattca
    11821 agtctatttt gtcagtttct tttctattta ttttttctat gctaactttc attaagttca
    11881 cctcaacata tcaccaaatt tattatacaa atctagcatt tgtttgtcaa gtatttttac
    11941 ttgataggca taggtttaat ttaattattt taccaatctt aatgtatctc tagctatcat
    12001 taattcttca ttagttggta ttaaaagcat ttttacttta gaatcagcag tagatattac
    12061 tgtttctttt cctctaacat cattagcttc tttatctaat ttcattccta aaaactccat
    12121 gttagaagca atatctgctc ttatagcctt tccattttca ccaacgcctg cagtaaatac
    12181 tacaacatcc aatccattca tttcagcagc ataagcgcct atatattttt gtactttttt
    12241 aacatacgct tctaatgtag cctgagctct ttcgtctcca tttttagctg catcttctat
    12301 atctctgaag tcactagaaa ttccagtcat tccatatact ccagattctt tattcattaa
    12361 tttatccaat ccatcagcat ctaaaccttc ttttctcatt aagaatggta atatagctgg
    12421 gtctatatct ccacatctag ttcccattat taatccttct aatggagtga atcccataga
    12481 tgtatctaca cattttccac catcaacagc agctatagaa gcaccatttc ctaagtgaca
    12541 agttactatt tttaaatctt ttatatcttt tcctaatatc tctgctgctc tctgagatac
    12601 ataattatga gaagttccgt ggaatccata tcttcttacc ccatatttag tatatagctc
    12661 atgaggtaaa ccatataagt atgaagattt aggcattgtt tgatggaaag cagtatcaaa
    12721 tacacctacc attggtacat ctggaagtat agctttacaa gcatctattc ccattatgtt
    12781 tgctggatta tgaagtggag ctaaatctat acattctttt aaagcttctt ctacttctgg
    12841 agttattact actgaagttg caaatttttc tccagcatgt acaactctat gtcctactgc
    12901 atctatttct ttcatttctt taactgcacc aaattctgga tgagcaacag cttctaaaac
    12961 taatgctatt gcatctttgt ggtccttcat tggttgttca acaacgtatt tatcatctct
    13021 acctgctttt tcatgtttta atatagaacc ttctattcct attctttcaa ccaatcctat
    13081 acataaaact tcttcattat tcatatctat taattgatat tttaatgaag aactcccaca
    13141 gtttaatact aatattttca ttattatttc ctccttagat ttttttataa ataatatgta
    13201 ataaattttg atttgtaata aatgtctaaa taaaac
//
LOCUS       DAMJWP010000088        13068 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.092, whole genome shotgun sequence.
ACCESSION   DAMJWP010000088 DAMJWP010000000
VERSION     DAMJWP010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13068
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.092"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(180..878)
                     /locus_tag="PC700_002100"
     CDS             complement(180..878)
                     /locus_tag="PC700_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2581411.1"
                     /translation="MSKKLILLVEDDKTIRKFISTALLTQDYDVKEAITGKEGISIAV
                     SYSPDVVLLDLGLEDMDGIEVIKAIRQFSNIPIIVVSAREQDRDKVEVFDAGADDYLT
                     KPFSIVELLARVRVAFRHSQVEAQQKDDVKSTFEVDKLLIDFDKRKVIVDDVEVHLTP
                     IEYNILSLLAKHHGKVLTHNFIIKEIWGSVIGNETKSLRVFMATLRRKIEKQPANPRY
                     IITEVGVGYRLNDE"
     gene            complement(912..3614)
                     /locus_tag="PC700_002101"
     CDS             complement(912..3614)
                     /locus_tag="PC700_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HDF2581412.1"
                     /translation="MDRPNPDILLEKIKNEEEKLSRGQLKIFFGYAAGVGKTYSMLES
                     AQNLKKVGVDVVVGYIEPHTRPETLALLDGLEILPVKEIEYKSIKLKEFDLDAALARK
                     PEVILVDEFAHSNVAGLRHTKRWQDIEELLLAGINVYTTVNVQHLESLNDIVEVITNV
                     SVKETIPDKFLDTNTQLELIDVEPDVLLERFNEGKIYKKEQATRAKNNFFIKDNLVAL
                     REIALRKTAERVNKEVQMTRLSKGDVTVIPTSDTLLACISPSPSSAKVIRTASRISDS
                     TFAKWIALYVETPNTARLPFDEQKQLQNNLKLAKKLGGEIIVLHGENIIEQILRIAKL
                     RNVTKIVIGRNHSSNKRFSKRFKKDIVDKLIDEVDYIDIHIIPYKFASDVKYKPKKDK
                     SSIKSKFKISKVDFIKLIFITLMISILAYAVQSMGFIRENILLIYMLGVVLVSMWTKG
                     YSTGIISSVFNIVLLNYFFTAPLYTLSIADSNYIVTLVVFSIVGIITSTLTSKIQHEA
                     ETAAKREENTKMIYQIIKGFLRLSSKDDIVNKGIELLSLSLSRDVICYLNVDKHNKSK
                     LYKKNTNNKDKNDLDSEDEKAVASWVLSNSTVAGNDTDTLPGSKGYYIPIVGMNLTLG
                     VVGISCIDSKLDTEDISLIETIIAQMAIALDREILSEAKENTNLEIERERLRSNLLRA
                     VSHDLRTPLAGISGAVSTIIKNKGTIGQDIIDELLSGVYEDTQWLIRLVENLLSMTKI
                     DEGKLEVKKHSELVEEIISEALQKIKKRIENALIDINIPEQILFVPMDAKLIEQVLIN
                     LIDNSLKYSKEDCKIEINVYEKDDYVWFEVSDNGPGISKELKKHIFDRFFTGEEGAKD
                     SRKGVGLGLSICKSIIQAHKGEIMVENNKDKGSTFKFSLPKENE"
     gene            complement(3792..5609)
                     /gene="ftsH"
                     /locus_tag="PC700_002102"
     CDS             complement(3792..5609)
                     /gene="ftsH"
                     /locus_tag="PC700_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDF2581413.1"
                     /translation="MDEHKPQKRSALYYYTVIIIVVMFLNALIFPSLMDKKMVEIDYG
                     KFLTMLDTGKVKSVEIENNRIAINPSSSKDKNIYITGRMDDPELVDRLKNAKVEFTKV
                     IPKENSPLLSILLTWIIPIGILMLFGNLMMKSMQKRMGGNAMQFGKSNAKMYVSAQSG
                     KRFNDVAGQDEAKEALTEIVDFLHNPEKYKKIGAQMPKGALLVGPPGTGKTLLAKAVA
                     GEANVPFFSISGSEFVEMFVGMGASRIRDLFKQAKEKAPCIVFIDEIDTIGKKRDNGS
                     GLGGNDEREQTLNQLLSEMDGFDGGIGVVILAATNRPDSLDKALLRPGRFDRRIPVEL
                     PDLGGREAILKVHAQNVNVSSDIDYNAIARSTSGASGAELANIVNEAALLAVKSGRDI
                     VEQQDFDESVETVIAGYQRKGAVISDKEKKIIAYHEIGHALVAAMQKHSAPVHKITII
                     PRTSGALGYTMQVAEDESVLMSKEEAIDKITTFTGGRAAEEVIFNTCTSGASNDIEQA
                     TKIARAMVTRLGMSEEFDMVALETVSNQYLGGDTSLACSSDTAAKIDEEVFKIVKACH
                     KNAIEILENNIDKLHELANYLLEKETITGDEFMEILNLN"
     gene            complement(5640..6083)
                     /locus_tag="PC700_002103"
     CDS             complement(5640..6083)
                     /locus_tag="PC700_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2581414.1"
                     /translation="MDEMSFKKELLELTRDINMKFTTLLSDFYQPLGITAVQALILSE
                     LCEHGEKKISDLGKNLNMTNSNVSVICQRLEKNGFLNRIRDIEDQRIVKVKVTNKSLD
                     IQEHISSSIFDSYFENMTSEKLQDMEDIIEGLEKLNKLLTYIDCK"
     gene            complement(6574..7464)
                     /gene="metA"
                     /locus_tag="PC700_002104"
     CDS             complement(6574..7464)
                     /gene="metA"
                     /locus_tag="PC700_002104"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HDF2581415.1"
                     /translation="MALILPKGLPVINKLLDEGIDVIYKEDFKKELKYEENIDTKIAI
                     LNLMPIKIDTELDLLRRIDKTGFNVSVEFIKISTRESKRSCNEYVKEFYKTFDEAKGE
                     YFDGFIITGAPVEQMEFEEVDYWNELEEIMDYSKRKTKSTLYICWAAQASLYKYYNVK
                     KLPLSQKCFGVFKHKVDKDSKIVDGFENEFFAPHSRHTTVNIEALKENKELSIVSHSK
                     EAGPYIITNSRDVFVMGHSEYDKYTLDKEYKRDINKGDKISIPQNYYINDDPSEEPTV
                     KWKKHSELLFRNWIKNYLIQ"
     gene            complement(7494..8759)
                     /locus_tag="PC700_002105"
     CDS             complement(7494..8759)
                     /locus_tag="PC700_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HDF2581416.1"
                     /translation="MYNKETICVQGNYKPGNGEPRVLPLYQSTTFKYSSIDQLAELFD
                     LKVDGHIYSRISNPTIQAFEEKISLLEGGVSSVAVSSGQSANMLAVLNICKSGDSILC
                     SSKVYGGTFNLLGPSLKKFGIDLISFDLDSSEDEIVELAKENTKVVFAETLANPTLEV
                     IDFEKIANVAKRINVPFIVDNSLASPVLCNPLKYGANIVTHSTTKYLDGHASSVGGII
                     VDGGNFNWDNGKFPELVEPDPTYHGISYTQKFGNAAYATKARVQLLRDYGNCLSPFNA
                     YLTNLNVETLHLRMERHSENALKIARFLEKHENVDWINYPGLEDNKYYENAKKYLSRG
                     CSGVLSFGVRGGLENAKKFVEKLQIASLVTHVSDVRTCVIHPASTTHRQLTEEQLIAS
                     GVLPSLIRLSVGIENVEDLIADLNQALNF"
     regulatory      complement(8869..8971)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(9025..11682)
                     /locus_tag="PC700_002106"
     CDS             complement(9025..11682)
                     /locus_tag="PC700_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDF2581417.1"
                     /translation="MENHKQMPWHSRATHEILESLDSSEEGLTDTEAEKRLKENGLNE
                     LRKKPPRTTLQMLWSQITDAMVMILIGAAILSLIFGEFTEAFVILIIVTVNAVIGIVQ
                     EKKAESSLEALKNMNSPTARVMRDGEENVIPASNLVVGDIVFLEDGAMVPADLRLIET
                     SSLKIQEASLTGESVPSEKDADTILPKECVLGDRSNMAYTSSIVTYGRGVGVVVATGM
                     DTEVGNIAHLLDSQDDFDTPIKRKLNTVGKTLSVIGIIVCVVIFAIGAFYQRPLLPMF
                     MIAISLAISIIPEGLPATATIVMALGVQRMAKRNALIRKLPAVETLGGATVICCDKTG
                     TLTLNKMTVTHIAVNGDFESGKVTSIDTASEKHPLVYKEIVYAGALCNDACINPDKTD
                     EILGDPTEGALIFMAKKFGIDQEALEEEYPRVFEQPFDSDRKCMTTVHNIDEKIIAYT
                     KGAVDEILDLCTKILTSKGERNITEVDKKNIHELCLNMSKDALRVLGFAKREISSIPK
                     EDSENIEYDLTFIGMVGMIDPPRTEVIDAVSTCKEAGIRTIMITGDHKVTATTIAHEL
                     GIWSEENTVISGDELDNFSDDELDEAVKNTTVFARVSPFDKLRIIQSLKRIGEVPAMT
                     GDGVNDSPALKSADIGIAMGISGTDVAKDSSDMILMDDSFTTIAYAIKEGRRVYRNIQ
                     KVIQFLLVGNIAEILTLFVAILLNWDTPLLAVHILWVNLATATLPALALGVDPPSRNI
                     MKHQPVKSTTLFEKNLIQRVVTQGIFVAIMTILAYKIGLNIENHSVGQTMAFCVLAFS
                     QMLRAFSQRSNTDSMFNRNNGKNPFLLISFLTSAFLIAVILFVPVFRNAFNLTVLNNL
                     EWLITIVLAIMSVIQVEVGKIIKRKKRKK"
     gene            complement(11810..12553)
                     /gene="yaaA"
                     /locus_tag="PC700_002107"
     CDS             complement(11810..12553)
                     /gene="yaaA"
                     /locus_tag="PC700_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HDF2581418.1"
                     /translation="MIIMLSPAKNMKNIEVFDRDLSLPCFIDNTKEIVENIKTFGIED
                     FKNKMKINEKLAVLNKNRFESIKFDRLGNPAILTYDGIQYKNIEAENFTRKDEEFANS
                     CIRIISGLYGVVKPYDSIYEYRLEMQTKLRVGEFKNLYEYWGNRIYKELIKEKTAIIN
                     LSSNEYSKSIEKFIKDSDTYITCTFKVNKNGILKVESTQAKKARGMMTKYIVKNRIRD
                     IEELKKFNLEGYKYKENLSNNSEYIFVKE"
     gene            complement(12563..13027)
                     /gene="bcp"
                     /locus_tag="PC700_002108"
     CDS             complement(12563..13027)
                     /gene="bcp"
                     /locus_tag="PC700_002108"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HDF2581419.1"
                     /translation="MLSIGTKAPEFTLEDKDGNKVSMSDFKGKKVVVYFYPKDNTPGC
                     TRQACAFRNAYDGFKKEDIQVIGISKDSIKSHQKFAEKHELPFILLSDPDLVAIKAFD
                     VWKEKKMYGKTALGVVRATYIIDENGIIEKVFEKAKPDTNAQEILEYLEKQE"
BASE COUNT         4040 a         2391 c         1510 g         5127 t
ORIGIN      
        1 agattacact atatacttat tttaaaacat atttcatatt attgatacta aaaagctaaa
       61 aaataaaatg catgtgctaa actgacaaca agcacataca cttcattctt attttaacta
      121 aactatataa ttttaatata tattaataag cacataatct aataattaat atattacaac
      181 tattcatcat ttaatctgta tcctacacca acttctgtaa ttatatatct tgggtttgca
      241 ggttgttttt ctatttttcg tcttaatgtt gccataaaga cccttaaaga cttagtctca
      301 ttccctataa cactacccca tatttctttt attataaaat tatgagtaag cacttttcca
      361 tgatgctttg caagtaatga aagaatatta tattcaatag gtgtcaaatg gacttcaaca
      421 tcatcaacaa taacttttct tttatcaaaa tcaatcaata atttatctac ttcaaacgta
      481 gatttaacat catctttttg ttgagcttct acttgtgaat gtctaaatgc gactcttact
      541 cttgctaata actctactat ggagaatggt tttgttaagt aatcatctgc ccctgcatca
      601 aatacttcta ccttatctct atcttgctct ctagctgaaa ctacaattat tggtatattt
      661 gaaaattgtc tgatagcttt tattacctct attccatcca tatcttctaa gcctaaatct
      721 aaaagaacaa catctggaga atatgagaca gcaatagata taccttcttt tccagttata
      781 gcctccttga catcataatc ctgtgttaaa agtgcagtag atataaattt tcttattgtc
      841 ttatcatcct ctactaacaa tataagtttt ttactcattt cttcctccaa aactaatttt
      901 aaaatttaaa actattcatt ttccttaggc aaactaaact taaaagttga acccttatct
      961 ttattgtttt ctaccataat ttcaccttta tgagcttgaa ttatagattt acaaattgat
     1021 aatccaagtc caacaccctt tctggaatct ttagctcctt cttctccagt aaaaaatctg
     1081 tcaaagatat gctttttaag ttcctttgat ataccaggac cattatcaga tacttcaaac
     1141 caaacataat catctttctc gtatacattt atctctatct tacaatcttc ctttgaatat
     1201 ttcaaagaat tatctattaa attaattaaa acttgttcta ttaattttgc atccatagga
     1261 acaaataata tctgttctgg tatattaata tctataagtg cattttcaat tctttttttt
     1321 attttttgta acgcttctga tataatttcc tcaaccagtt cagaatgttt tttaacttcc
     1381 aatttccctt catctatctt ggtcatacta agtaaatttt caacaagtct tattaaccac
     1441 tgagtatctt catatacacc actaagtaat tcatctatta tatcttgacc tatagtaccc
     1501 ttgtttttta taattgtact tactgcacca gatattcctg ctagtggagt tctaaggtca
     1561 tgcgaaactg ctcttaaaag attacttctt aatctttccc tttcaatctc aagattggta
     1621 ttttctttag cttctgacaa gatttctcta tcaagcgcta ttgccatttg agcaattatt
     1681 gtttcaatta agcttatatc ttctgtatcc aattttgaat caatacatga tattcctact
     1741 actcctaaag ttaaattcat tcctacaatt ggtatatagt atccctttga accaggaagt
     1801 gtatccgtgt catttcctgc cactgttgaa ttggataaaa cccaacttgc aacagctttt
     1861 tcatcttcac tatccaaatc atttttatcc ttattattag tatttttttt gtaaagtttt
     1921 gatttattat gcttatctac atttaaataa caaattacat ctctacttaa gctaagagac
     1981 aaaagttcta tacctttgtt tactatatca tctttacttg ataatcttaa aaatccctta
     2041 attatttgat atatcatttt tgtattttct tctcttttag cagcagtttc tgcttcatgc
     2101 tgtatcttag acgtaagagt gctagttatt atacctacta ttgagaatac aaccagtgtt
     2161 actatatagt ttgaatctgc tatagataat gtatataatg gtgctgtaaa aaaataattt
     2221 aaaagaacta tattaaatac agaacttatt atacctgtac tatacccttt agtccacata
     2281 gaaactaaaa ctacacccaa catatatatt aacaatatat tttctcttat aaatcccatg
     2341 ctttgtactg catatgctaa aatacttatc atcaaagtaa taaatataag ttttataaaa
     2401 tcaactttgc taattttaaa tttagatttt attgatgatt tatccttttt aggtttgtat
     2461 ttaacatctg aggcaaattt atatggaatt atatgtatat ctatataatc aacttcatca
     2521 attaatttgt caactatatc ttttttaaat cttttactaa atcttttatt acttgaatga
     2581 tttctaccta ttacaatttt tgttacattt ctcagttttg caattctaag tatttgctct
     2641 attatatttt caccatgtaa aacaattatt tctccaccta atttcttagc taattttagg
     2701 ttattttgta gttgtttttg ttcatcaaat ggaagtcttg ctgtgtttgg agtttcaaca
     2761 tataaagcta tccattttgc aaatgtactg tctgatattc tggatgcagt tctaataact
     2821 tttgctgaag atggagatgg acttatacat gcaagaagtg tatctgatgt aggtattaca
     2881 gttacatcac cctttgatag tctggtcatt tgaacttctt tattaactct ctctgctgtt
     2941 tttctaagtg cgatttctct aagtgcaacc aggttatctt ttataaaaaa attatttttt
     3001 gctctagtag cttgttcttt cttatatatc ttaccttcat tgaacctttc aagaagtaca
     3061 tcaggttcaa catctataag ttctaactgt gtatttgtat ccaaaaactt atctggtata
     3121 gtctctttaa ctgatacgtt tgtaattacc tcaacaatgt catttagact ttctaaatgt
     3181 tgtacattta cagtagtgta gacatttatg cctgctaaca gcaattcttc tatatcttgc
     3241 catctttttg tatgccttaa cccagcaaca ttagaatggg caaattcatc tactaatata
     3301 acttctggtt ttcttgctaa agcagcatct aaatcaaatt cttttagttt tatagattta
     3361 tattctatct cctttacagg aagaatttcc aagccatcaa gtagagccaa tgtttctggt
     3421 ctagtgtgtg gttcaatata tccaactact acatctacac ccactttttt taaattttgt
     3481 gcagattcca acatagagta ggtttttcct actccagctg cataaccaaa gaatattttt
     3541 aattgacctc tacttagttt ttcttcttca tttttaattt tttctaatag aatatcagga
     3601 ttaggtctat ccattaaaaa acctccttac accattcatt actattataa tatcaattcc
     3661 gatataaaat tgtagttaag atgtattttt aatgattaag attatgtaaa gatttaaata
     3721 tgaatttaaa aaatttagtt gtaaaataaa aatgcctaac aataggcatt tttatcttgt
     3781 gtataatcat tttagtttaa gttaagtatt tccataaact catcacctgt aatagtttct
     3841 ttttctaata aataattagc aagttcatgc aatttatcaa tattattttc taagatttca
     3901 attgcatttt tgtgacaagc ctttactatt ttaaatacct cttcatctat tttagcagca
     3961 gtgtcggaag aacaagcaag agaagtatct cctcctaaat attgattgct tacagtttca
     4021 agagcgacca tatcaaactc ttcactcata ccaaggcgag taaccatagc tcttgcaatt
     4081 ttagtagctt gttctatatc atttgatgct cctgatgtac aagtgttaaa gataacctct
     4141 tcagcagcac gaccacctgt aaaagtagtt attttatcaa ttgcttcttc tttagacata
     4201 agtacactct catcttcagc aacttgcata gtatatccca atgctcctga agttctaggt
     4261 attatagtaa tcttatggac aggtgcagaa tgtttttgca tagcagcaac aagtgcatga
     4321 cctatttcat gatatgctat aattttcttt tccttatctg aaattacagc tcctttacgt
     4381 tgatacccag caattacagt ttcaacagat tcatcaaaat cctgttgttc tactatatct
     4441 ctgccagact taactgctaa aagtgctgct tcgtttacaa tatttgcaag ttctgcacct
     4501 gatgctcctg atgtagacct tgcaattgca ttataatcta tatctgagct tacatttaca
     4561 ttttgagcat gtacctttaa tatagcttca cgtcctccaa ggtctggaag ttcaactggt
     4621 atacgtctat caaatcttcc tggacgaagt aatgctttgt caagactgtc aggtctattt
     4681 gtagcagcaa gtattactac tccaattcca ccatcaaaac catccatctc acttaacaac
     4741 tgatttaaag tttgttcacg ttcatcattt ccacctaatc cacttccatt atcacgcttt
     4801 ttaccaatag tatcaatctc atctataaac actatacatg gagctttttc ttttgcttgt
     4861 ttaaataaat cacgaatcct agaagctccc attcctacaa acatctcaac aaactcagaa
     4921 ccagatattg agaaaaatgg tacatttgct tcacctgcta cagcttttgc aaggagtgtt
     4981 tttcctgtac ctggaggtcc tactaaaagt gctcctttag gcatttgtgc tcctatcttt
     5041 ttatactttt ctggattgtg taagaaatct actatttcag ttaaagcttc ttttgcctca
     5101 tcttgaccag ctacatcatt aaatcttttt ccactttgag cagatacata cattttggcg
     5161 ttgctttttc caaattgcat agcattacca cccattcgct tttgcataga cttcatcatt
     5221 aagttaccaa atagcataag aatacctata ggtattatcc atgtaagtaa aatacttaaa
     5281 agaggtgaat tttccttagg aataacctta gtaaattcta cttttgcatt ttttagtcta
     5341 tctaccaatt ctgggtcatc cattctacca gttatgtata tattcttatc cttgctacta
     5401 gatggattta ttgctattct attattttct atctcaacag atttaacctt tcctgtatcc
     5461 aacattgtga gaaactttcc atagtcaatc tcaaccattt ttttatccat taaacttgga
     5521 aatattaaag catttaaaaa cattactact attattataa ctgtataata atagagtgct
     5581 gacctttttt ggggtttatg ttcatccata attttattct cctttaaatt atttttatgt
     5641 tatttgcaat caatgtaagt taaaagttta tttaatttct ctaaaccttc tattatatct
     5701 tccatatctt gcaatttttc actggtcata ttttcaaaat aactatcaaa aatactactt
     5761 gaaatatgtt cttgtatatc caatgattta tttgtaactt ttacttttac aattcgttgg
     5821 tcttctatat ctcttatgcg atttaaaaag ccattttttt caagcctttg acagattact
     5881 gatacattac tatttgtcat gtttaagttc ttccctaaat cgcttatttt tttctctcca
     5941 tgttcacata actcagataa aatcaaagct tgcactgctg taataccaag tggctgataa
     6001 aaatcactta ataaagttgt aaatttcata tttatatctc tagtcaactc tagtagctct
     6061 ttcttaaaac tcatctcatc catacaaacg ctccctacga aaatacttta tttcaaattt
     6121 aggctgaagt gcatactgta aataaatact taatactata atgtttaatg tattaaatat
     6181 tatagtatta agtatttatt gtcaattaca atattgtaac ttcaattgga attttgttat
     6241 cttattgata tttaatttta tacaggatat ttttaataaa atttaataaa aaatcgatat
     6301 atatatttta cactgatttc tcccttttta atctctttta aaagttaaaa tacaaattta
     6361 ttgatactat aaaggtttag tctcccatta tcgcaccata tataaaatta aaaccacctc
     6421 tatctacata cacctaaagt gattttctta aaatataaaa ctactattat aaggttaaat
     6481 ttaaaaaaaa taaggtataa agtatcatta agattaactt tatacctata aatttctata
     6541 tttttttagt taacatttat atactcttaa aatttattgt atcaaatagt tttttatcca
     6601 atttctaaac aacaattctg aatgtttttt ccattttaca gttggctctt ctgaagggtc
     6661 atcattaata tagtaatttt gaggtataga tattttatct cctttattta tatccctctt
     6721 atattcttta tcaagagtgt atttgtcata ctcgctatga cccattacga acacatctct
     6781 tgaatttgta attatatagg gaccagcttc tttagaatga ctcacaatgc tcaactcttt
     6841 attctctttc aaagcttcaa tatttacagt agtatgtctt gaatgtggag caaaaaattc
     6901 attttcaaat ccatctacta ttttactatc tttatctacc ttatgcttaa atactccaaa
     6961 acatttttga cttagtggaa gtttttttac attgtagtat ttgtaaagac ttgcttgagc
     7021 tgcccaacat atatataatg tagatttagt ttttctctta gaataatcca taatttcttc
     7081 tagttcattc caataatcaa cttcttcaaa ctccatttgt tcaactggag ctcctgttat
     7141 tataaagcca tcaaaatact ctcctttggc ttcatcaaat gttttataaa attctttaac
     7201 atattcatta caacttcttt tactttctct tgttgaaatt ttaataaatt caacagacac
     7261 gttgaatcct gttttatcta tccttcttag taaatctaat tctgtatcta tctttatagg
     7321 cattagattt agtatagcta ttttagtgtc tatgttttct tcatacttta attccttttt
     7381 aaaatcttct ttataaatta catctattcc ttcatctaat aatttattaa taacaggtaa
     7441 tccctttggt agtattaaag ccatatcttt tcacttctct ttctcttaga atattagaaa
     7501 tttaaagctt gatttaaatc agctattaaa tcctctacat tttctattcc aacagataat
     7561 cttattagtg aaggcaatac tccagatgca attaattgtt cttctgttaa ttgtctatga
     7621 gtagttgaag ctggatgtat aacacaagtt cttacatctg aaacatgtgt aaccaaagat
     7681 gctatctgta atttttccac aaattttttg gcattttcta acccacctct tactccaaat
     7741 gataaaacac cactacatcc tcttgataaa tactttttgg cattctcata atacttgtta
     7801 tcttcaagtc ctgggtaatt aatccaatct acattttcat gtttttctaa aaatctagct
     7861 attttaagtg cattttcact atgtctctcc attctaagat gtagtgtttc aacatttaaa
     7921 ttagtaagat acgcattgaa tgggcttaaa caatttccat agtctctaag caactgaact
     7981 cttgctttag ttgcatatgc ggcatttcca aatttttgag tatagcttat tccatgatat
     8041 gttgggtctg gctcaactaa ttctggaaat tttccattat cccagttaaa gtttccacca
     8101 tccactataa ttcctccaac acttgaagca tgcccatcta aatattttgt ggtagaatga
     8161 gtaactatat ttgctccata ctttaaaggg ttacaaagca ctggagatgc taatgaatta
     8221 tcaacaataa atggaacatt aattctctta gctacatttg ctattttttc aaaatctatg
     8281 acttcaagag tcggatttgc aagtgtttct gcaaatacaa ccttagtatt ttcctttgca
     8341 agttctacta tctcatcttc acttgaatct aagtcaaacg atattaaatc tataccaaat
     8401 tttttaagac taggtcctag taaattgaat gttcctccat atacttttga agaacaaagt
     8461 atactatctc ctgatttaca tatatttaaa actgccaaca tatttgcaga ttgccctgat
     8521 gatacagcta cagaagatac tccaccctct agtaaactta ttttttcttc aaaagcttga
     8581 atagtaggat tgcttattct tgaatatata tgtccatcaa cttttaaatc aaataattca
     8641 gcaagttggt ctatactgct atatttaaaa gttgtacttt gatataaagg taatactctt
     8701 ggttctccat tacctggttt ataatttcct tgcacacata ttgtttcttt attatacatt
     8761 cttgtatccc ccttagattt tagtaaaaaa aattaaacta tttatcagat gagagttaaa
     8821 ataaataaag cctgagtata taactcaggc tctgtataag cttaattata ctcatctctc
     8881 agctttcgcc gcaggattta gcaccaatgt atataaatat actggttgcc gggtttcata
     8941 gggccagtcc ctccacctct ctggataaga tttatttaat ttttctatgt tttatatctt
     9001 atatattttt cctaattttg tcaactattt ttttcttttt tttcttttaa taattttccc
     9061 aacctctact tgaattacag acattatggc taaaacaatc gttataagcc actctaagtt
     9121 atttaaaact gttaagttaa aagcatttct aaaaacaggt acaaacagta ttactgctat
     9181 taaaaatgca gaagttaaaa atgagattaa aagaaatgga tttttaccat tatttctatt
     9241 aaacattgaa tctgtatttg aacgctgact aaatgctcgt aacatctgtg aaaatgctaa
     9301 tacacaaaat gccattgttt gacctacaga atgattttct atattaagac caattttata
     9361 tgctaaaata gtcattattg ccacaaatat accttgggta acaactcttt gtataagatt
     9421 tttttcaaac aaagtggttg atttcactgg ctggtgcttc attatatttc tgcttggtgg
     9481 gtcaactcca agagcaaggg ctggaagcgt agcagttgca aggtttaccc atagaatatg
     9541 aacagcaaga agcggagtat cccaatttaa taatattgct acaaacaacg taagtatctc
     9601 tgctatatta ccaacaagca aaaattgtat aactttttgt atgtttctat atacacgtct
     9661 accttctttt attgcataag caattgttgt aaaactatcg tccattaaaa tcatgtcaga
     9721 agaatctttt gcaacatctg taccacttat acccatggct ataccaatat cagcagactt
     9781 taaggcagga gagtcattta caccatcccc agtcattgct ggtacttctc caattctttt
     9841 tagagattgt ataattctaa gtttatcaaa cggggacaca cgagcaaata ctgtagtatt
     9901 tttaacagct tcatctagtt catcatcact aaaattatca agttcatcac cagatatcac
     9961 agtattttct tcactccata ttcctaactc atgagcgatt gttgtggcag taactttatg
    10021 gtcacctgtt atcattatgg ttcttatacc tgcttcttta caagtactta ctgcatcaat
    10081 tacctcagtt cttggtgggt caatcatacc aaccatacct ataaatgtca aatcatattc
    10141 tatattttca ctatcttctt ttggtataga tgaaatctca cgcttagcaa aaccaagtac
    10201 acgcaatgca tctttagaca tgttaagaca taattcgtgt atattctttt tatccacttc
    10261 ggtaatgttt ctttcacctt ttgaagttaa tattttagta cataggtcga gaatttcgtc
    10321 tactgctcct ttagtataag caataatctt ttcatctata ttatgaacag ttgtcataca
    10381 ttttctatct gaatcaaaag gctgttcaaa tacacgtgga tattcttctt caagagcttc
    10441 ttgatctatt ccaaattttt tggccataaa aattaatgcg ccctctgttg ggtcacctaa
    10501 tatttcatct gttttatctg gattaataca tgcatcatta caaagtgcac cagcataaac
    10561 aatctcttta tacactaatg gatgtttttc agatgcagta tctattgatg tcacttttcc
    10621 agattcaaaa tctccattga cagcaatatg tgtaacagtc attttattta aggttaatgt
    10681 accagtttta tcacaacata ttactgtagc tcctcctaat gtttcaacag caggtaattt
    10741 tctaattaaa gcatttcttt tagccatacg ctgtacgcct aaagccatta caatagtagc
    10801 tgttgcaggt aatccctctg gaataattga tatggcaagg gaaattgcaa tcataaacat
    10861 tggcaagaga ggtctctgat aaaatgcacc tattgcaaat ataacaacgc atacaataat
    10921 acctatgaca cttaatgtct ttccaacagt attaagttta cgttttatag gagtatcaaa
    10981 atcatcttgt gaatctaaaa gatgtgctat gttacccact tctgtatcca taccagttgc
    11041 tactacaaca cctacacctc taccatatgt gactattgaa gatgtatacg ccatattgct
    11101 acggtctcct aatacacatt ctttaggtaa gattgtatct gcatcttttt ctgatggaac
    11161 agattctcct gttaaagaag cctcttgaat ttttaaactt gatgtttcta ttaaacgaag
    11221 gtctgctgga accatagctc catcttctaa aaaaacaata tctcctacta caagattact
    11281 tgctggaata acattttctt caccatcacg cataacacga gctgtaggtg agttcatatt
    11341 ttttaatgct tctaatgatg attctgcttt tttctcttga acaattccaa taactgcatt
    11401 tactgttaca attattagta ttacaaaagc ttctgtaaat tcgccaaata tcaaagataa
    11461 tattgctgct cctattagta tcataaccat agcatctgta atttgactcc aaagcatttg
    11521 taaagttgtt ctaggtggtt tttttctaag ttcatttaag ccattctctt taagccgttt
    11581 ttccgcctcg gtatcggtga gcccctcctc tgatgaatct agactttcta aaatttcatg
    11641 cgttgctctt gaatgccaag gcatctgttt gtgattttcc attttggaag accactcctt
    11701 tcttttttta atttaagaat aattatattt taaaatcaag ttcaattcaa tacacactaa
    11761 gtatatttta cctaaattta acaatgccat ttaatattta acaaaacttc tactctttta
    11821 caaatatgta ttcactattg ttagataagt tttctttata cttataacct tctaaattaa
    11881 actttttcag ttcttcaata tctcttatcc tattttttac aatgtatttt gtcatcatac
    11941 ctctagcttt ctttgcttgt gtagattcca ctttcaatat gccattttta tttactttaa
    12001 aagtacatgt aatatatgta tcactatctt taataaattt ttctatggac ttgctgtatt
    12061 catttgatga aagattaatg atagctgttt tttctttgat tagttcttta tatatacgat
    12121 ttccccaata ttcgtataaa ttcttaaact cacctactcg taattttgtc tgcatttcta
    12181 atctatattc atatatacta tcatatggtt ttacaacacc atacaatcca cttatgattc
    12241 gaatacagct atttgcaaac tcttcatctt tccttgtaaa attttctgct tctatatttt
    12301 tatattgtat tccatcataa gttaagattg cagggtttcc taatctatca aatttaatgc
    12361 tttcaaatct atttttattt aatacagcta atttttcatt aattttcatt ttatttttaa
    12421 aatcttctat accaaatgtc tttatatttt ccactatttc ttttgtgtta tctataaaac
    12481 aaggcaaact caaatctcta tcaaatactt ctatattctt catattttta gctggtgata
    12541 acattataat cataaattct tattactcct gtttttccaa atattcaaga atctcttgtg
    12601 catttgtgtc tggttttgct ttttcaaaga ctttttcaat aattccattt tcatctataa
    12661 tatatgtcgc acgtacaact cccaatgcag tttttccata catttttttc tctttccata
    12721 catcaaaagc ttttatagca actaagtcag ggtcagaaag taaaataaaa ggaagttcat
    12781 gcttttctgc aaatttttga tgtgatttta tactatcttt acttatccca ataacttgta
    12841 tatcttcctt tttaaatcca tcatatgcat ttctaaaagc acaagcttga cgtgtacatc
    12901 ctggtgtatt atctttagga taaaaatata ctactacttt tttgccttta aaatctgaca
    12961 tacttacctt atttccatct ttatcttcta gtgtaaattc tggtgctttt gttcctatac
    13021 tcaacataaa tatctctcct tttgttttta ttaataattt tatactat
//
LOCUS       DAMJWP010000089        13003 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.093, whole genome shotgun sequence.
ACCESSION   DAMJWP010000089 DAMJWP010000000
VERSION     DAMJWP010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13003
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.093"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            79..960
                     /locus_tag="PC700_002109"
     CDS             79..960
                     /locus_tag="PC700_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2581420.1"
                     /translation="MNLYHLRYFVTLAHLEHYTKAAENLSITQPSLSHAISLLENELG
                     VALFEKEGRNIVLTKYGKIFLKDVEKSLEILDSSVKSLKITGTGEGQIDLAFLRTLGT
                     DFIPDIVHKFLKSNPAKSIDFKFHTGVTTDIIQGLKERKYDIAFCSKLEKEKGIEFIP
                     VAKQDLVLIVPYSHPLAAKDTIDLKETIPYPQIVFNQRSGLRYIIDDMFKKINQQPNI
                     VYEVEEDQVIAGLVAKNFGIAVVPNMNMLSFTKVKVIQIIHPSWERNFYLAFIKDRYL
                     PPAIKNFKNFVIKNAQL"
     gene            complement(1168..2031)
                     /locus_tag="PC700_002110"
     CDS             complement(1168..2031)
                     /locus_tag="PC700_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HDF2581421.1"
                     /translation="MLSSYVKRVSKIIKKKYIMFIDESGRADINHEDPFTLTGVIFEY
                     KYAVSQGDYVCSLRKEMDAFKEYCFGTSNIGIHLTDISRKSRAFEKFDDSQIKLFYDE
                     LPLFLSRLECTIISVTVDKARLKKYYAPSKEPYVVAFIHVLQNFYSFVNNETVESARI
                     VIESRDDVSNLKVQKAFFDVFNNGTIYLDIDEELRDKVKGFIIAKKEDADYREGLEIA
                     DILCNPLSRARRGLIEANPKCMQYGEKNKIFKSVRNKIYTPTSVNDIRNWGFKEVPIV
                     EPVGPWLEKMR"
     gene            complement(2186..4120)
                     /locus_tag="PC700_002111"
     CDS             complement(2186..4120)
                     /locus_tag="PC700_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp22"
                     /protein_id="HDF2581422.1"
                     /translation="MKKSLKKYLVLALTLVLVFACGVPESSAASKHVIIVNSRKNTLG
                     YFVNNKLVKEFRVATGKKGSETPTGKTKVVNKIKNRPYYKGNIPGGSPRNPLGDRWMG
                     LALKGTYGDTYGIHGNNNESSIGKHISGGCIRMHNKDVRWLFDQVPVGSDVIIDYSND
                     SYVKIAAKYKINLNQTGWKTENGKKYYVKSDGTYQKNSWLKVNGKMYYFDASGVMQTG
                     WKAINNKKYYLGTDGARVSGWKVIDGKTYYFNSDGVMQTGWQEKNGKKYYLGSDGLSV
                     TGWQEIDGNKYYFDKTGIMQTGWQQIDGKSYYLDKDGKMLTGSQKIDGKDYTFNEDGT
                     INPTWDTIIGANRFDTAKKISSVGNWNADSSDTVILVNGNAIADGITATPLASSYDST
                     ILLTNTANLPTETVEEMKLLAPKTVILIGGENAISSKLEQEIKTTFNTETKRIAGQDR
                     YQTATRIAEELGNREEIKTAYMVSGNGEADALSVASKAGEEKQPIILVNKDGITEESY
                     KWLTERKLENAYFIGGPSAINDSVIAKMNDITTEDISGNRIYGDSRVDTNAKVIEKFY
                     GDTDLQAVLVSKSDALVDALSAGPLAVKLHSPIVLMDNSGLSLEQQRVFANKKVETPY
                     QIGGGVSYIVMDKLMDILAK"
     gene            complement(4232..5245)
                     /gene="galE"
                     /locus_tag="PC700_002112"
     CDS             complement(4232..5245)
                     /gene="galE"
                     /locus_tag="PC700_002112"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDF2581423.1"
                     /translation="MAVLVAGGAGYIGSHTAIELLESGYEVVIVDNLSNSNSIVVDRI
                     KELSKKPVKFYNIDIRNKDEMHVVFKENNIESIIHFAALKAVGESVEKPIEYYSNNLI
                     STLNLFELMREYGVKKFVFSSSATVYGDPHTCPILEDFPLSVTNPYGRTKLMIEQMLV
                     DISKADKSLDIALLRYFNPVGAHKSGRIGEEPNGVPSNLMPYITKIAVGKLKELSVYG
                     NDYPTHDGTGVRDYIHVLDLAAGHVKALQKLEENPGLVVYNLGTGKGYSVLDLVKAFS
                     KASGKEIPYKIVGRRAGDVAMCYADSSKAEKELGWKAKYELEEMCEDSWRWQSMNPNG
                     YEE"
     gene            complement(5270..6139)
                     /gene="galU"
                     /locus_tag="PC700_002113"
     CDS             complement(5270..6139)
                     /gene="galU"
                     /locus_tag="PC700_002113"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDF2581424.1"
                     /translation="MTVKKAIIPAAGLGTRFLPATKSQPKEMLPIVDKPTLQYIIEEA
                     IESGIEEILIITGRNKKSIEDHFDKSVELELELEQKGKTEMLEMVRDISNMVNIHYIR
                     QKEPKGLGHAIYCAKSFIGDEPFAVLLGDDIVDSDVPCLKQLIDTYNEYKTTVLGVQK
                     IAKEDTNKYGILDVKHIEDRVYKVKDMVEKPAIEEAPSDIAILGRYIITPAIFSILEK
                     QTPGKGGEIQLTDALQTLGKQEAIYAYNFEGRRYDVGDKIGFLEATIDFALKRENLKD
                     DLMDYMRKKVSEK"
     gene            complement(6337..6657)
                     /locus_tag="PC700_002114"
     CDS             complement(6337..6657)
                     /locus_tag="PC700_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581425.1"
                     /translation="MSSKYNVWTFNYEFLGLDSGDEKNNEVYYEIDLDEILNENYIDE
                     DIDSGLDSDGNSILGELISEMDIDEDVSVDMTYQELEVDLEDLDSYLDEDIDSDIKGI
                     LDEI"
     gene            complement(6647..8638)
                     /locus_tag="PC700_002115"
     CDS             complement(6647..8638)
                     /locus_tag="PC700_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581426.1"
                     /translation="MLKLGEKIIYELSDGFELSGISRYSKEYKKLHITNLGNIIISKS
                     DEVNEDTGVKYIYSFSDNKEKLEAVLEDSQIIVYGNNLPFYIKSENSSKNVTANLTMK
                     ITLDEYDLICKSQREFIFYLKDNMTILSLDDNKFYMGGINKDNEKFIFISGKNRFEIN
                     FDDIERYILEDKRVSLKGYFHMEREGIIVRSVSIFNNNIDSVVPSDLNERVKDNQKIG
                     NLPKDCDIVFCKISGNIDGFDYKNTNMLLVKYQDQLIFINKKSKKTIVKSAKDNCSKL
                     NLGEDIILYDNKNVFNLHINDKNREIMQIDDLKDIENEIVGYTLKHAPFFIQEDFDSL
                     TILKSFQKEIISIKNSDIKDIVINKELENENSNFVETEIKFNNQKVLLNLSKNMVQKL
                     MQDVFIYAKQPLLKENSIEVIYKNWSKAMNDMIIFNFFGNIYYMKSEFDKILEKELND
                     EIRIEVINSLYKQIQEQRNNLDLLSAYMPRILENQEIDLFEKYNTKLDVQVFKQIKNL
                     LSDLSYNISSYLNEVEKSLDNIIFVISGEDKKKYNYRMLKESESASLDVFLKQAISRL
                     NHLVENMYPYYVDETSREMFKLFELLWKNYRNIDDDSIKEILFERITNTYVFKQLTLN
                     NSTKERRKDIIEKIYNSVDYGTNKLDENMFFTGGIKYVK"
     gene            complement(8760..11303)
                     /locus_tag="PC700_002116"
     CDS             complement(8760..11303)
                     /locus_tag="PC700_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HDF2581427.1"
                     /translation="MKVLSENNIIRNTVLNVTASFLKQESKINEKLDGVLEKKFEKVE
                     FNEAKYAELLKFNILFYKTLARNTEPLIGKWIVDKYIPEIDELEKDLELTTAKCRKYV
                     NKAMKEGLDSLKANDLNSFLAYDKMELSERRRRLEKDYKVLNLYKDLLNISLRKISLE
                     KKECGDLFLKNQADAKRELKREIIFCVNKILASNKDVVPNNVEENNEQDKVEIKATEI
                     EDLQLGKDNSENDMLLNEIKNSKVKHESKSNDIESKFIDKLNSLNNESISQTIHNEYK
                     KLCGLEELHSVEGYGFGKEIIKDFACATVVLEFLKRRNRDLIEGAMRLTIIGEFGPEN
                     FKEFVDYVIKNKTEISEDTWNKAQNLIKDNYSELENHEIASKRTRRNKDIDIEEYIYM
                     IKNADKDICFRSSISIEDDAEEGAKEEVDSNNQDIGDVVEDKDTTDKEYDSNKEDIIE
                     PENKKSKKKAKLFGFIKKDNEEVEQEEENLNDISPDIILDKPVENHQAKSEEIEQNEL
                     KEIKQEEPSQHIEEERSVKIEKPINNNLDEKVSSNNESKLEKESKNLEDKKAKEIKEE
                     KLENEKSVVIPIKKKENSNKKSKNSSKDKYRENKKEMKNYVSDKEDSLDDEEVVSKKS
                     RLKETIIAVVIVVIVGVGYFITVGNNKKNDKENIPKSSTQQQANNKLTEEEKKAQAEK
                     EKKEAEEKAKAEKEAQEKAQAEKKAKEMEAYKDGKGVYYTVYAGSLKVEKTAKETAKE
                     YEAKGISSTIIQENGYYKIKIGDYSQYGEAQEKCNELAKKSIDTYIAMYDKYYDYKLE
                     ELKESAPSLSAEELKQKYEDLRSELKNKSGYREYVKHLDKLYEEIVEGA"
     gene            complement(11581..11915)
                     /gene="rnpB"
                     /locus_tag="PC700_002117"
     ncRNA           complement(11581..11915)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PC700_002117"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            12047..12724
                     /gene="srtB"
                     /locus_tag="PC700_002118"
     CDS             12047..12724
                     /gene="srtB"
                     /locus_tag="PC700_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtB"
                     /protein_id="HDF2581428.1"
                     /translation="MKKLYRIVINIILVLVILYSGFNIYSKLTKYNHDTKTSSELQKK
                     EYKKEDLSKINSDFKFWLSVENTNINYPVVQSKDNSYYLDKDFYKKDSISGTLFMDYR
                     NKSIDDKNIIIYGHNMKNKTMFNNLNKFKDADFFKNNNKIKITLNGKEFLYDVFSAYI
                     VESDYDYLKTNFNNESDYQNYINDITSKSLYKSPIKVNSNDKIVTLSTCTYEFDDARM
                     VIHGRLI"
     gene            complement(12884..12958)
                     /locus_tag="PC700_002119"
     tRNA            complement(12884..12958)
                     /locus_tag="PC700_002119"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12924..12926),aa:Thr,seq:tgt)
BASE COUNT         4007 a         2286 c         1326 g         5384 t
ORIGIN      
        1 ataaaaatgt aaaaatatct taaaggttaa cttctacaaa tatataaata aaaataccta
       61 tgaaaggatg aacaaaaatt gaatttatat cacttacgct attttgttac attggcacat
      121 ttagaacact atacaaaagc ggcagaaaat ttatctataa cacaaccaag cttaagtcat
      181 gctatttctt tactagaaaa tgaacttggg gtggctctat ttgaaaaaga aggtagaaat
      241 atagtcctta ctaagtatgg aaaaatattt ttaaaagatg tggaaaaatc attagaaatt
      301 ttagattcaa gtgtaaaatc attaaaaata actggtactg gtgaaggaca aatagactta
      361 gcttttctta gaacacttgg tactgatttt atcccagaca tagtgcataa atttttaaaa
      421 tctaatcctg ctaaatcaat agattttaaa tttcatactg gtgtaactac tgacattatt
      481 caaggtctta aagaaagaaa atatgatatt gcattttgtt cgaaacttga aaaagaaaag
      541 ggaattgaat ttatacctgt tgccaaacaa gatttagtac taattgtacc atatagtcat
      601 ccactagctg caaaggatac tatagattta aaagaaacca taccttatcc tcaaatagta
      661 tttaatcaga gaagtggtct tagatatata attgatgata tgtttaaaaa aataaatcaa
      721 cagcctaata tagtctatga agtagaggaa gaccaagtaa ttgctggatt agttgcaaaa
      781 aatttcggaa ttgctgtagt cccaaatatg aatatgctta gctttacaaa agtaaaagta
      841 attcagatta ttcacccaag ttgggaaaga aacttttact tagcatttat aaaagataga
      901 tatttgcctc ctgcaattaa aaactttaaa aactttgtca ttaaaaacgc tcaattataa
      961 tttattaata aaggggttat ttttaaatat cctcttttat tctttttata aaataaaaaa
     1021 atctatactt ttaaaagtat agattcctaa ctgacagtcg gcatccgagt ctcagtaatt
     1081 atcgttatac aacagcaact ggattcagtt cctgaataac taggttattt acattatatc
     1141 agattttttt aaatttgtca attgtatcta acgcattttt tcaagccatg gtccaacagg
     1201 ctctacaata ggaacttctt taaatcccca atttcttata tcattcacac tcgttggagt
     1261 atatattttg tttctgacag atttgaatat tttgtttttt tctccatatt gcatacactt
     1321 tggattagct tctattaacc ctcttcttgc tctgctaaga ggattacata aaatatctgc
     1381 tatttctaaa ccttctctat agtcagcatc ttcttttttg gcaattataa aacccttaac
     1441 cttatctctt aattcttcat ctatatctaa gtatattgtt ccattattaa atacatcaaa
     1501 aaacgccttt tgtactttta agtttgagac atcatccctt gactctataa ctattcgtgc
     1561 agattctaca gtttcattat ttacaaatga ataaaaattc tgaagaacat gtataaaagc
     1621 cacaacatac ggttcctttg aaggtgcata atatttcttt aatctagctt tatctactgt
     1681 tactgatatt atagtacact ctaatctaga tagaaataaa ggcaactcat catagaataa
     1741 cttaatctgt gaatcatcaa acttctcaaa tgctcttgat tttcttgata tatctgtcaa
     1801 atgtattcct atgttactgg ttccaaaaca atactcttta aatgcatcca tttctttcct
     1861 caaagaacat acataatcgc cttgacttac tgcatattta tattcaaaaa ttactcctgt
     1921 caatgtaaat gggtcttcat gatttatatc agctcttcca ctttcatcaa taaacattat
     1981 atattttttc tttataatct tactcaccct cttaacatat gatgatagca tctatattca
     2041 ttttatatga gttttaaaaa tattccaaga tttttatata acttaaactt aattttatac
     2101 taaaaaattg agatgtaaaa caatcaagct tttattgatt gaatttcaca tctcaattat
     2161 aattattttt caatatatat ttttactatt ttgcaagaat atccattaat ttatccatta
     2221 ctatataaga tactccgcct cctatttgat aaggagtttc aactttttta tttgcaaata
     2281 ctctttgttg ctctaatgat aatcctgaat tatccattaa aactattggt gaatgtaatt
     2341 ttacagctaa tggtccagct gataaagcat ctactaaagc atctgattta ctaactaata
     2401 cagcctgtaa atcagtatct ccatagaatt tttctattac tttggcatta gtatctactc
     2461 tactatctcc atatattcta tttcctgaaa tatcttcagt agttatatca ttcatttttg
     2521 ctattactga atcgttaatt gcacttggtc cacctataaa gtatgcattt tctaattttc
     2581 tttctgttag ccatttataa gattcttctg taattccatc tttattgact aatattatag
     2641 gttgtttttc ttccccagct ttagaagcta cagataaagc atctgcttcc ccatttccag
     2701 aaaccatata tgcagttttt atttcttctc gatttccaag ttcttcagct attcttgtag
     2761 cagtttgata tctatcttga cctgctattc tctttgtctc tgtgttgaaa gtagttttta
     2821 tttcttgctc taatttagaa cttattgcat tttctccacc tatcaagatt actgttttag
     2881 gtgctaataa cttcatttct tcaacagttt cagttggtaa attagctgta ttagtaagta
     2941 gtattgttga gtcatagcta cttgctaaag gagttgctgt tataccatca gctattgcat
     3001 taccatttac taatattaca gtatctgaac tatcagcatt ccagtttcca actgaactta
     3061 tctttttagc tgtatcaaat ctatttgcac ctataattgt atcccatgta ggatttatag
     3121 ttccatcttc attaaaagta taatctttcc catctatttt ttgagaacca gttagcatct
     3181 ttccatcttt gtctaaatag taagacttac catctatttg ttgccatcct gtctgcatta
     3241 ttcctgtttt gtcaaagtaa tacttattac catctatttc ttgccatcct gttactgata
     3301 aaccatcaga tcctagatag tatttctttc cattcttttc ttgccatcct gtttgcatta
     3361 ctccatctga gttaaagtaa taagtttttc cgtctattac tttccatcca gatactcttg
     3421 ctccatcagt tcctaaataa tattttttgt tattaattgc tttccatcct gtttgcatta
     3481 ctcctgaagc atcaaagtaa tacatttttc catttacttt tagccaacta ttcttttggt
     3541 atgttccatc agattttaca taatacttct taccattttc tgttttccat cctgtttgat
     3601 ttagatttat cttatatttt gccgctatct ttacatagct gtcattacta taatctataa
     3661 ttacatctga acctacagga acttggtcaa ataaccatct aacatcttta ttgtgcattc
     3721 ttatacatcc accagaaata tgctttccta tagaactctc attattattt ccatgtattc
     3781 catatgtatc tccataagtt ccttttaatg ctaatcccat ccatctatct cctaatggat
     3841 ttcttggact tcctccaggt atattacctt tataatatgg tctatttttt atcttgttaa
     3901 ctacttttgt tttccctgtt ggtgtttctg accccttttt acctgttgca actctaaatt
     3961 cttttacaag tttattattt acaaaatatc ctagagtatt ctttcttgaa tttactataa
     4021 tcacatgttt actggctgcg cttgattctg gtacgccaca tgcaaatact aaaactaaag
     4081 tcagtgctag tactaaatac ttttttagac tttttttcac tataacttcc ccctctttta
     4141 gaaacattac tacatgtgtc taattataac ataaaattac caatacctta tatttacaaa
     4201 tttgttacat ttatacatat aagtatgtat tctactcttc atacccatta ggattcatac
     4261 tttgccatct ccatgaatct tcacacatct cttcaagttc atacttagct ttccatccaa
     4321 gctctttttc tgcctttgaa gaatctgcat aacacatggc tacatctcca gcccttcttc
     4381 ctacaatctt atatggtatt tcttttccac ttgctttaga aaatgctttt actaaatcaa
     4441 gaacactata accctttcca gttcctaaat tataaacaac aagtcctgga ttttcttcta
     4501 atttttgtaa tgcttttaca tgaccagcag ctaaatctaa tacatgtata tagtctctaa
     4561 ctccagttcc atcatgtgtt ggataatcat taccataaac actcaattcc ttcaatttac
     4621 ctactgctat ttttgttata taaggcatta gattgctcgg aactccattt ggttcttcac
     4681 ctattctacc acttttatga gcaccaactg gattaaagta tcttaaaaga gctatatcca
     4741 atgacttatc tgcttttgat atatctacta acatctgctc tatcataagt ttagttcttc
     4801 catatggatt ggttacagaa agtgggaaat cctctaatat aggacaagta tgtgggtctc
     4861 catatacagt agctgaagaa ctaaatacaa actttttaac accatattct ctcattaatt
     4921 caaataaatt taaggtactt attagattat tgctgtaata ttcaattggt ttttctactg
     4981 attcaccaac tgcttttaaa gctgcaaagt gtataattga ttcaatatta ttttctttaa
     5041 atacaacatg catttcatct ttatttctga tatcaatatt ataaaatttt actggttttt
     5101 tagataattc cttaattcta tcaaccacta ttgagttgct attacttaag ttatcaacta
     5161 taacaacttc ataacctgat tctaaaagtt ctattgctgt atggcttcct atatatcctg
     5221 ctcctccagc tactaaaact gccattgtaa ttacctcttt tctaatattt tatttttcac
     5281 ttactttttt tctcatatag tccattaaat catcttttaa attttctctc tttagagcaa
     5341 agtctattgt tgcctctaaa aaaccaatct tatctcccac atcatatctt ctaccttcaa
     5401 agttataagc ataaatagcc tcttgttttc caagagtttg aagtgcatct gtcagttgta
     5461 tctctccacc tttaccaggt gtctgtttct ctaatatact aaatattgca ggagttatta
     5521 tatatcttcc aagtatagct atatcagatg gagcttcttc aattgcaggt ttttcaacca
     5581 tatctttaac tttatatact ctatcttcga tatgttttac atccaatata ccatatttat
     5641 tagtatcttc tttagctatc ttttgaacac ctaatacagt tgttttatac tcattataag
     5701 tatctattaa ttgttttaag cacggaacat ctgaatctac tatatcatca cctaataata
     5761 cggcaaaagg ttcatcacct ataaaacttt ttgcacagta aattgcatgt ccaagacctt
     5821 ttggctcttt ttgtcttata taatgaatat ttaccatatt tgatatgtct cttaccattt
     5881 ctaacatctc tgtcttacct ttttgctcta gttctaactc taattcaact gatttatcaa
     5941 aatgatcttc tatagatttt ttatttctac ctgttattat aagaatttct tcaattcccg
     6001 attctatagc ttcttctatt atatactgta aagttggttt atctactata ggaagcattt
     6061 ctttgggctg tgattttgtt gctggtaaaa atctggttcc tagccctgct gctggtatta
     6121 ttgctttttt aactgtcatc tgcatttttt cacccttctt cgtaaatttt ctattgtacc
     6181 aaattaatat accatattta tactttttct tcaaactcta tattatccca atatttaatg
     6241 ctataagata atataaggta gatgaaattt catctacctt tagttttata agttaatatt
     6301 gtattgttta tattttatcc aaaacttata caggtactaa atttcatcta agatgccttt
     6361 tatatcacta tctatatctt catctagata agaatctaaa tcttctaagt caacttctag
     6421 ttcttgatat gtcatatcaa cactgacatc ctcatctata tccatttctg atattaattc
     6481 tcctaaaata ctatttccat cagaatctaa accactatct atatcttcat ctatatagtt
     6541 ttcatttaaa atttcatcta aatcaatctc gtaataaact tcattatttt tttcatcccc
     6601 tgagtccaat cctaagaact catagttaaa agtccaaaca ttgtatttac ttgacatact
     6661 ttattcctcc tgtgaaaaac atattttcat ctagtttatt ggttccataa tcaacagagt
     6721 tatatatttt ttcaattatg tcttttcttc tttcttttgt actattattt aatgtcaatt
     6781 gtttaaatac atatgtattt gttattctct cgaataatat ttcttttata ctatcatcat
     6841 caatatttct atagtttttc cacaataact caaataactt aaacatttcc ctactagttt
     6901 catctacata ataaggatac atattttcta ctaaatggtt taatcttgat atggcttgtt
     6961 ttaaaaatac atcaagactt gcactttcac tttcttttaa cattctataa ttgtatttct
     7021 tcttatcttc tcctgaaatt acaaagatga tattgtctaa acttttttca acttcattta
     7081 aataactgct tatattatat gataagtcag acaatagatt tttaatctgc ttaaacactt
     7141 gtacatcaag tttagtatta tacttttcaa acaaatctat ttcttgattt tctaatattc
     7201 ttggcatata tgctgacaat aaatctaaat tgtttctttg ttcttgtatt tgcttataaa
     7261 gtgaatttat aacttctatt cttatttcat catttaactc tttttctaaa atcttatcaa
     7321 attcagattt catatagtaa atatttccaa aaaaattaaa tattatcata tcattcatag
     7381 ctttagacca attcttatat atcacttcaa tagaattttc tttaagtaga ggttgctttg
     7441 catatataaa tacatcttgc ataagttttt gaaccatatt tttacttaag tttaaaagta
     7501 ctttttgatt attaaatttt atttccgttt ctacaaaatt agaattttcg ttttcaagtt
     7561 ctttattaat tacaatgtct tttatatcac tattttttat agatatgatt tctttttgaa
     7621 aagactttag aatagtcaag ctatcgaaat cttcttgaat aaaaaatgga gcatgtttta
     7681 atgtatatcc cactatttca ttttctatat cttttaagtc atctatttgc ataatttctc
     7741 tatttttatc atttatatgt aggttaaata catttttatt atcatataat attatatctt
     7801 ctcctaaatt taacttagaa caattatctt tcgctgactt tacaatagtc ttcttagatt
     7861 tcttatttat aaaaattaat tggtcttgat attttactaa aagcatgttt gtgtttttat
     7921 aatcaaatcc atctatattt cctgatatct tgcagaatac tatatcacaa tctttaggaa
     7981 gatttcctat tttttggtta tctttaactc tctcatttaa atcagatgga actacactat
     8041 ctatattgtt attaaatata gatacagacc ttactataat cccctctctt tccatatgaa
     8101 agtatccctt caatgacact cttttatctt ctaaaatgta tctttctata tcatcaaaat
     8161 ttatttcaaa tctatttttc ccactgataa atataaactt ctcattatct ttattaattc
     8221 cacccatata aaacttattg tcatctaaac ttaatatagt catattatcc tttagataga
     8281 atataaattc tctttgactt ttgcagatta aatcatattc atccaaagtt attttcatag
     8341 tcaaatttgc tgtaacattt ttacttgaat tttcactttt tatgtaaaaa ggcaagttat
     8401 ttccatagac aattatttga gaatcttcta aaactgcctc taatttttct ttattgtctg
     8461 aaaaactata tatatatttt acacctgtat cctcattaac ttcatctgat ttgctgatta
     8521 ttatattccc tagatttgtt atatgaagct ttttatattc ttttgagtat ctacttatgc
     8581 cactaagctc aaacccatca gaaagctcat atatgatttt ttctcctaat ttcaacatac
     8641 cttcaccacc taaacagaat tataagtatt ttataaaggt gaataaaatg tctcaaaaca
     8701 aataatattt ttttaatact ttttattctg agacatagtt tctccttttt tataatgctc
     8761 taagcacctt caactatttc ttcataaagt ttatctaaat gtttaacata ttcacgatat
     8821 ccagatttgt tctttaactc acttcttaaa tcttcatatt tttgtttaag ttcttcagca
     8881 ctcaaagaag gagctgattc ttttaattct tctaacttat aatcatagta cttatcatac
     8941 atagctatat atgtatcaat agatttctta gctagttcat tacatttttc ttgtgcttct
     9001 ccatattgag aataatcacc tatttttatt ttatagtatc cattttcttg aattattgtt
     9061 gaagaaatac ctttagcctc atattctttt gctgtttctt ttgctgtttt ttcaacttta
     9121 agtgaacctg cataaacagt gtaatataca cctttaccat ctttatatgc ttccatttct
     9181 tttgctttct tttcagcttg tgctttttct tgagcctctt tttcagcttt tgccttttct
     9241 tctgcttctt ttttctcttt ttcagcttgt gcttttttct cttcctcagt taatttattg
     9301 ttagcttgtt gttgagttga tgactttgga atattctctt tatcattttt cttattgttg
     9361 cctactgtta taaaatatcc tacaccaaca ataaccacta taactactgc tataatagtt
     9421 tcttttaatc tactcttctt agaaacaacc tcttcatcat ctaaagaatc ttctttatct
     9481 gatacataat ttttcatttc tttcttgttt tctctatatt tgtccttaga agaattttta
     9541 gattttttat tactattttc tttcttttta ataggtatta ctactgattt ttcattttct
     9601 aatttttctt cttttatttc ttttgctttt ttatcttcta agtttttact ttctttttca
     9661 agcttcgatt cattatttga agatactttt tcatctaagt tattatttat tggtttttct
     9721 atttttacac tacgttcttc ttctatatgt tgacttggtt cttcttgttt tatttctttt
     9781 aattcatttt gctctatttc ttctgatttt gcttgatgat tttcaactgg tttatctaaa
     9841 attatatcag gagaaatatc atttaaattc tcttcttctt gttcaacttc ttcattgtct
     9901 tttttaataa atccaaataa tttagccttc tttttactct ttttattttc tggttcaatt
     9961 atatcttctt tattgctgtc gtactcttta tctgtagtat ctttatcttc tacaacgtca
    10021 cctatatctt gattattact atctacttct tctttagccc cttcttcagc atcatcttct
    10081 attgaaatac tacttctaaa gcaaatatct ttatctgcat tttttatcat gtaaatgtat
    10141 tcttcaatat cgatatcttt atttcttcta gttctttttg atgcaatttc atgattttct
    10201 aattcactat agttatcttt tattagattc tgtgctttat tccatgtatc ttcactaatt
    10261 tctgttttat ttttaattac atagtccaca aattccttaa aattttcagg accaaattct
    10321 ccaattatag tcagtctcat tgcaccttct ataaggtctc tatttcttct ctttaaaaat
    10381 tctaatacaa ccgttgcaca agcaaaatct ttaataattt ctttgccaaa accatatcct
    10441 tcaacagaat gaagttcttc taatccacat aacttcttat attcattgtg tattgtttga
    10501 gatatacttt cattatttaa gctatttaac ttatctataa acttcgattc tatatcattt
    10561 gattttgatt catgttttac ttttgaattt ttaatttcat ttagtagcat gtcattttct
    10621 gaattatctt tacctaattg caaatcctca atttcagttg cttttatttc tactttgtct
    10681 tgttcgttat tctcttccac attattaggt accacatctt tattgctagc taatattttg
    10741 tttacacaga atataatttc tctttttaat tctcttttcg catctgcttg atttttaaga
    10801 aataaatctc cacattcttt cttttctaaa cttatttttc taagagatat attaaggagg
    10861 tctttatata ggtttaaaac cttataatcc ttttctaaac gtcttcttct ctctgatagt
    10921 tccattttat cataagctaa aaaactattt aaatcattgg cttttagaga gtctaatccc
    10981 tctttcatag ccttatttac atactttcta cattttgctg tagttaattc taaatctttt
    11041 tctaattcat ctatttcagg tatgtactta tctactatcc atttgccaat aagtggttct
    11101 gtatttcttg ctaatgtctt ataaaacaat atattaaatt ttaaaagctc tgcatatttt
    11161 gcttcattaa attctacttt ttcaaatttc ttttccaaaa caccatctag cttttcattt
    11221 attttagact cttgttttaa aaaacttgct gtgacattta aaacagtgtt tcttattata
    11281 ttattttcac tcaaaacctt cactccttaa atcaattatt ttattgtaat atttacattg
    11341 tgcaaagttg ctaatttgat tatgatatct aaataattat ttactaaatt tgctacatct
    11401 tatatcatat catattattt acatcatttt actttttctt tttatatcta tcttatatta
    11461 taccattttt aatatctgta ctaacttata ttacaatatt cctcaaaatt ttaaaagtag
    11521 attagacaaa tttacaaata aaaaaacatc agtttaaact gatgtttttg actatcgaaa
    11581 taattaagca agtctataag cggagttctg tattagataa tcatctttct aggcctattg
    11641 tcaccaatag gctcaagcga cctacctacc ggagagtacg agcagcactc tttattccta
    11701 tcttggtctt gctccaggtg gggtttacat agccaactag tcgcctagct gctggtgagc
    11761 tcttacctcg cctttccatc cttaccacaa tagtggcggt ttatttctgt tgcactatcc
    11821 ttaggatcgc tcccactagg cgttacctag caccctgctc tatggagctc cgactttcct
    11881 catgtaagat tacatgcgat tatctggctt acttaatatt tattttactt agttatgtta
    11941 acacaaattc ttttacatgt caaagtaata ttatactaaa aatttaaatt tattcaaaaa
    12001 tagtgtaata tagatatata aactccattt aactcgaggt gatatattga aaaaattata
    12061 tagaatagta attaacatta tactagtatt agtaatactt tacagtggat ttaatatata
    12121 ctcaaaactt accaaataca atcatgatac taaaacatcc tcagaacttc aaaaaaaaga
    12181 atataaaaaa gaggatttat caaagattaa cagtgatttt aaattttggc tttctgttga
    12241 gaatacaaat ataaattatc cagttgtaca atctaaagat aattcttact atcttgacaa
    12301 agatttttat aaaaaagatt ctatttctgg tacactgttt atggattata gaaataaatc
    12361 tattgatgac aaaaatatca taatatatgg acataatatg aaaaataaaa ctatgttcaa
    12421 caacctaaat aagtttaaag atgctgactt ctttaaaaac aataataaaa taaaaataac
    12481 cttgaatgga aaagaatttt tatatgatgt tttttctgct tatattgtag aatctgatta
    12541 tgattatcta aaaacaaatt ttaacaatga atctgattat caaaattaca taaatgatat
    12601 tacttctaaa tcattgtata aatctcctat taaggtaaat agcaatgata aaatagttac
    12661 actttctaca tgtacctatg aatttgatga tgccagaatg gtgattcatg gtagattgat
    12721 ttaaaactca cagacacagt ttaaaacaat aattttaaac tgtgtcatct tttataccta
    12781 taatttataa attaggccac taaaagtatg taatacaaaa taacaaatac atcaaacaat
    12841 aaaaaacaat aaaaaaagac tatgctaaaa acatagtctt tactggagct ggtgatagga
    12901 atcgaaccta caacctgctg attacaagtc agctgctcta ccgttgagcc acaccagcac
    12961 acctattcta ttgtaaatta gtaaaactaa tttttaaaag tta
//
LOCUS       DAMJWP010000090        13001 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.094, whole genome shotgun sequence.
ACCESSION   DAMJWP010000090 DAMJWP010000000
VERSION     DAMJWP010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13001
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.094"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(153..530)
                     /locus_tag="PC700_002120"
     CDS             complement(153..530)
                     /locus_tag="PC700_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDF2581429.1"
                     /translation="MSKVVIATEKAPGAIGPYSQAIQVGNIVSTSGQLPIDMKTGELV
                     SDPKEATKASLDNVKAILEQAGTSMDKIFKTTVFVKDLKDFNDINEVYATYFKENPPA
                     RSCVQVAQLPKDAVLEIEAMAVL"
     gene            1381..1866
                     /locus_tag="PC700_002121"
     CDS             1381..1866
                     /locus_tag="PC700_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581430.1"
                     /translation="MSKSELTAETTEEMLEVLSGKDYDIACHLHELGKSLDCKIEPKT
                     GARSYKIVYSTKKPKRSLFTIECNEKKWRVKANLFHLNTYKDAVEECSKTIKDSITKT
                     RTCKKCNSKCIGGSSFDLDGKSYLTCIGSGHYFANMEEMDWKNLEKLITKENNIMQES
                     V"
     gene            complement(2066..2605)
                     /locus_tag="PC700_002122"
     CDS             complement(2066..2605)
                     /locus_tag="PC700_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2581431.1"
                     /translation="MSKNVLIVSASPRKGGNSDLLCDQFIQGVLESKNQVEKIFLKDK
                     DINYCTGCGACYEKEASCSQTDDMGEILEKMIGADVIVLATPVYFYTMNAQMKTMIDR
                     TCSRYTEICDKDFYFIVAAADENVADMERTIEGFRGFTSCLNGSKEKGIVYGVGAWNV
                     GDIKGSKAMKQAYEMGKLA"
     gene            complement(2598..3011)
                     /locus_tag="PC700_002123"
     CDS             complement(2598..3011)
                     /locus_tag="PC700_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2581432.1"
                     /translation="MTISEVSKKYELSADTLRYYERIGLIPPVNRNKSGIRSFTEEDC
                     EWVNFIKCMRGAGLSIETLIEYVAMFQQGSSTIKARKELLIEQRNQLAKRIEEMQKTL
                     ERLNFKIDRYEEGIIEKEKVLKSSRNKKMEAVSYE"
     gene            complement(3047..4168)
                     /locus_tag="PC700_002124"
     CDS             complement(3047..4168)
                     /locus_tag="PC700_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDF2581433.1"
                     /translation="MEAKKLGFGLMRLPIKDENDVTTIDMEHLNKMVDTFLERGFTYF
                     DTAYVYHMGKSEIALRESLVKRHKRESFTIATKLPLMALKKKEEQETIFNEQLEKCGV
                     DYFDYYLLHNIGVSHYEVAKKFDSFKFIEEKKKEGKIKNIGFSFHDSAELLDKVLSEH
                     PEVDFVQLQINYLDWDNESIQSRKCYEVAEKHNKPVIVMEPVKGGTLAKIPEKAEKLL
                     NDYNPDMSISSWAIRFAASKDNVMMVLSGMSNMEQLLDNTGYMQNFKPFVQEEYNIID
                     EAVEIINESILVPCTACQYCVEGCPKNIAIPNYFALYNAEKQALNTGFSTQMVYYNNY
                     TKTYGKASDCIECKQCEESCPQHIKIIDALKNVAETFEK"
     gene            4767..6419
                     /locus_tag="PC700_002125"
     CDS             4767..6419
                     /locus_tag="PC700_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp21"
                     /protein_id="HDF2581434.1"
                     /translation="MNNKNLAILISGMMFFSSSSAIFADNTTKQERLIGKTRYETAVE
                     VSKLGWVQSKTAIIVNGNSIQSALCANPFAKLKNAPILLVNNNSIENSTKAELKRLGV
                     DNVYIVDSGNSISSKVENEIKSLNIKINKIVGNNIYKMSTNVLKEIDKIKKVENVAVV
                     NGLKGLSDAVSIASPAAIHNMPIILVSDENSNDSSINFLNNRSIKKSYIVGGNKILSD
                     NTVKKYPNRERISGSDRNNTNAKIIDKFYKDKKINNVFVAKDGINQENALVDSLSIGV
                     LAAKENSPIMISNGSLVNSQKEFIKNKDIDEITQVGGGYNQKAFEDILKLKNVNIRNK
                     TKKISLIQAKEILKQALPGSDILVCYYDEDDNEYDCKVIKDNILYEIEIDAYSGKIIE
                     IEKDDDLNDNNLGTQNISLDKAKSIALDAVPNGNLIYCKYDAEDNEYEAKVIKNKKTY
                     ELEISAFDGRIVDIDMDNDSDDDFDDNDKNNSNTNDNSSIISAEKAKSIMLDKVPGGK
                     IVKFKYDEDDKEYEGEIIKGKIEYEITINAITGKILEFEIDD"
     gene            6716..7465
                     /locus_tag="PC700_002126"
     CDS             6716..7465
                     /locus_tag="PC700_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005340256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2581435.1"
                     /translation="MNTILKVNNITKTYNKGQQPSLDKVSFNVSEGEFIGIMGASGSG
                     KTTLLNVLSTIDKPTSGDIFINNINLKSLNDTCAADFRKDNLGFIFQEYFLLDSLTIF
                     ENISVPLTLQKLNPDKIENMVKSLAERFDIASQLNKYPNELSGGQRQRASAVRAIVKH
                     PSILFADEPTGALDSNSATELLHSLQEANEELKTTILMVTHDAYAASFANRILIFKDG
                     HIIKELNKQPNNRHTFYELIIQEIAKLDTKR"
     gene            7478..9505
                     /locus_tag="PC700_002127"
     CDS             7478..9505
                     /locus_tag="PC700_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2581436.1"
                     /translation="MNIFYMAIKNLKKNFSFYSLYLLSVSFVITVFFAFTSFSVNKIM
                     LEKISTDGRVESMCNVISIFLMAFVIFYMAYSNRFFLRRRTKELGIYALLGYRKFTIL
                     SLLTFENVLICSGAFITGLVLGALSHKGIVFGITALLNLSIDNSQIPFFNINAINKTI
                     IFIALVVLVLAISNGKFLYKTSLVKLIRHEKSAEKKLKFRKIPAFMGFIMILSGYCLS
                     LDILRGAKSVWVSIGFYQMGLITMIIIVIGTVLFINSFLPYIMQKSKKNKRTFYTDMK
                     IIAIPNFIYRIRSNSKTLIMLTLLSATTLTVSSVMALSLYYPIASVSRIAPSEIEFRV
                     EKNKQIEDVKQIVSQYTSNTDDIIFTQTNIYKVKSSSKDLPFEYEIGTSKNNLSNSSS
                     SKEVISRESGFECISFSDYYTLLKAQNRTKLLKQLTPLRDNECILMKYQPNNDGYSEN
                     GTIYPLLINGKETPVAVRETSLNNAISFANSVGTLIVNDSIYEQIAQNKLPMTSILSI
                     NGKSIKNNENLYSNISDYLDGSPYLQGYSHRINDLLSINSSTFLLIGFLVVLFFIAVG
                     SILYFNNISAISDSTSDYKILQKMGYDNIQIKKIIKKQVLPFFNIPFLLGILDCIFAT
                     LVYKTALMQNLLGNSFSQYIPVIIAIFLTSVVYLIYYILTIKSCCKIVLKN"
     gene            9649..11232
                     /locus_tag="PC700_002128"
     CDS             9649..11232
                     /locus_tag="PC700_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2581437.1"
                     /translation="MTNFNLKILFLGNYILSLLFMGISTSTETYMNIQSIIPLIISTL
                     LLIDITYITSCNVRENNIISLFCGLLALDSWYLLLSFVTTPIGTIIFKILSTVIVYVS
                     IKFIFLFLFQGSGYNFRKITDILLLITCIGAIVGAFISNRTYACMYGIQFVVSIISFL
                     YIIFYHLKRVVFVIKSEKKPISISVFITIIGFLTYYFFTLRISNHIGNFGIYLTVLVF
                     FMSIHGIALKENDSFPISTVFNIKQRAIFLFISILVISLVVLFANGNFSIILIAINVL
                     FMLIFLCNIILEFNLRDEKNKVVKNSKYTFALEKLQQEEQLKTEFSNFLHDDILQNLL
                     SIKNMMSKSYRPEVQDIIYETLNNLNTCIRNQMQDYHPVILKNLTVKENYQNLIESVS
                     SSFPQKNICVSFKCSDTLFLVEPYDILIYRLIKELLTNVYKHSNGSHAWIALALEKNI
                     IKLNINDNGVKNTSFKTPLDITKITFDIIKHKGIISIKEQVENLGGTISISDNIPHGV
                     CVQITIPMKGDGSYKYFVS"
     gene            11204..11824
                     /locus_tag="PC700_002129"
     CDS             11204..11824
                     /locus_tag="PC700_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2581438.1"
                     /translation="MVLINILLVDDHMLFAKSLSIALEEYEEIEQFYSTQDIHNLSEM
                     IQDKKIDIVLMDINLRGLSECDGLKVAYNLLETNSTIKIIILTGYDLPVYKHEAKKIG
                     IYGFLNKNISPDSLIDSLLKVYQGVTCFPSENTEVIIEDLTNMEKRILQLICSGKKRK
                     IIADELYISERTLSNHLQHIYDKLDVSSSVEAITKAIKMGYISPVY"
     gene            complement(12012..12938)
                     /locus_tag="PC700_002130"
     CDS             complement(12012..12938)
                     /locus_tag="PC700_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HDF2581439.1"
                     /translation="MSNSFYQLNSAVLYFNEKRDVNRIRFNHYVMRKENIQRLKGTLP
                     RGQFMMTVRKAASDLDVSISTISRLVNEFIDLGILRLISRGVKGNCCSVYSYVCSENS
                     NAEIVTKNSDIFKNIEKYIDATINNNQKTMKYKHRDELFKNSNTNTYSRINQESIDKD
                     YDLIIHTGYETENETKKKELLKNNLNKKNLNKNTFENLDEDLTEDSTYKNPESIHEII
                     IRKLNSESGKNFKADLSITKALIDKRLKEGYSLEDFYKVIEAKVRRWRYTDMEIYIEP
                     TILFGYEFESYLNEESSLSACNYGDMCYEDMY"
BASE COUNT         4702 a         1838 c         1677 g         4784 t
ORIGIN      
        1 aaaaatatac attatatgag aaaagctcaa tagttaaata tttagataga tttttatata
       61 gactttttta atataaactt ttttaattgt agactttttt tattagtttt taatttttat
      121 tagttttgtt attggtttta ttgttataat acttataata ctgccattgc ttcaatttct
      181 aaaactgcat cttttggtaa ttgagcaact tgtacgcatg accttgctgg tggattttct
      241 ttaaaatatg tagcatatac ttcattaata tcattaaaat cctttaaatc ttttacgaat
      301 actgtagtct taaatatttt atccatactt gtaccagctt gttctaatat tgcttttaca
      361 ttatctaaag aagcttttgt tgcttctttt gggtcagata ctaactcgcc agtcttcata
      421 tctattggaa gttgacctga tgtagaaact atgtttccaa cttgaattgc ttgagaatat
      481 ggtccaattg ctcctggagc tttctctgta gctattacta cttttgacat gacttcacct
      541 cctattatta agttatattt acaggaaaat aaaatttcct aaacaccaat attaatacga
      601 acctttagat taaatttcaa atctaaaact tcaaataatc aatacttaga tgattatgtt
      661 tatttgtgtc ttgttaactt ttattaaagc aattaacgtg ccaaacttta tttatatata
      721 aaattttgtg aagtcattca aaatagttac ttctgaattt tttcgtaaaa aaatgcttta
      781 aaattacttt tttatgatgt caattttttt ctcatatcta gaaatttacc aacatgagaa
      841 ttcttgtttt gagaatttac agattaaagt tatttttcaa tcttaaatta tcaagttatt
      901 acatatgaaa gaactatagt tgacaatccc actattataa ataataaaac ctttacccat
      961 accttattta ccctagttat catagagcct attgaaaata taacagctaa atataataat
     1021 aaccaaaaaa ttatcaatat ctaccactgt tgctgaaaat ccaaaaaact accttttgat
     1081 agtattactt aacaatctaa ttaatctcat aataaattta caacttataa ataaattgtt
     1141 atattggccg tatctcaaca actttctcct ttcctagttt taaatctttt atagaattgt
     1201 accacgtcta agtaaatttt catactatta agtcttattt ctaataaatc attagctctc
     1261 attctatgcc caataatgta agattgtagt aattttattg attttaatag ttttatttaa
     1321 tatacttata ttaattattt tcatgtgaaa ttattactta tataggggga tttttttaat
     1381 atgagcaaaa gtgaattaac ggcagaaaca acagaagaaa tgttagaagt actaagtggt
     1441 aaagattatg atattgcatg tcatttacat gaacttggta aatcattaga ttgtaaaatt
     1501 gaaccaaaaa caggtgctcg ttcttacaaa atagtatatt caactaagaa accaaaacgt
     1561 agcttattta ctattgaatg taatgaaaaa aaatggagag ttaaagcaaa tctttttcat
     1621 ctaaatacat ataaagatgc tgtggaggaa tgctctaaaa ctattaaaga tagtataact
     1681 aaaactcgta cttgtaagaa atgtaattca aagtgtattg gaggttcttc gtttgattta
     1741 gatggaaagt cttatctgac ttgcatagga agtggtcatt attttgcaaa tatggaagaa
     1801 atggattgga aaaacctaga aaaattaatt actaaagaaa ataatattat gcaggaatct
     1861 gtatagtttt ttaagggtaa ttattaaaat atatgagtaa caacagatac ttaaataggc
     1921 ataaaaaaat aaagggtata ataaggatat agaataaaaa taaatgatat agcacaaaga
     1981 ggctgtctca aaatatagat aagcctcttt ttattgttga aatgataaga atatattcga
     2041 ttttatacaa ttttatacaa acatactaag ccaacttacc catttcatat gcctgtttca
     2101 ttgccttact tcccttaata tcacctacat tccaagcacc tacaccatat actatacctt
     2161 tttctttaga accatttaga catgatgtaa atcctctaaa tccttctatt gttctttcca
     2221 tatcagccac attttcatct gctgctgcaa cgataaaata gaaatcttta tcacaaattt
     2281 cagtatatct agagcaagtt ctatcaatca tagttttcat ttgcgcattc atagtataaa
     2341 aataaacggg agtcgctaaa acaatcacat ctgccccaat cattttttca agaatttctc
     2401 ccatgtcatc tgtctgtgaa caacttgctt ctttttcata acaagcacca catccagtac
     2461 aatagttaat atctttatct tttaaaaata tcttctccac ttgattttta gattctaata
     2521 ccccttgtat aaactggtca cacaataagt cagaatttcc accttttcta ggactagctg
     2581 atactattaa tacattttta ctcataagat actgcctcca ttttcttatt tctactgctc
     2641 tttaatactt tttctttctc aataatacct tcttcatatc tatcaatctt aaaatttaat
     2701 cgttccaaag ttttttgcat ttcttctatt ctttttgcta gttggtttct ctgctctatt
     2761 aaaagttctt ttctagcttt gatagtactg ctcccttgtt gaaacatagc tacatactca
     2821 attaatgtct caatagaaag acctgcacct ctcatacact ttatgaaatt aacccactca
     2881 caatcttctt ctgtaaaaga cctaattcca cttttatttc tatttacagg tggtattaaa
     2941 ccaattcgtt cataatatcg taaagtatcc gctgaaagct cgtatttttt actaacttct
     3001 gaaatcgtca tacatatctt ctcctcttga tattgtatag attgaattat ttctcaaagg
     3061 tttctgctac attctttaaa gcatctataa ttttaatgtg ttgaggacag ctttcttcac
     3121 attgtttaca ttcaatacaa tctgaggcct ttccataagt tttagtataa ttattatagt
     3181 aaaccatctg tgttgaaaat ccagtattaa gagcttgttt ttctgcattg tacagcgcaa
     3241 aatagtttgg aatagcgata ttttttggac agccttctac acaatactga caggcagtac
     3301 aaggaactaa aattgattcg ttgataatct ctactgcttc atctataata ttatattcct
     3361 cttgtacgaa tggtttgaaa ttttgcatat atcctgtatt gtctaataat tgttccatat
     3421 ttgacatccc acttaagacc atcataacat tatctttact tgctgcaaaa cgtattgccc
     3481 aagatgaaat tgacatatct ggattgtagt cattcaataa tttctctgct ttttctggta
     3541 tctttgcaag tgtacctcct tttactggct ccataacaat tacaggctta ttatgcttct
     3601 ctgccacctc gtagcatttt ctagattgta tgctttcatt atcccaatct aaataattaa
     3661 tctgtaattg cacgaagtct acttctggat gctcactcaa gactttatcc aatagttctg
     3721 cactatcatg aaaagaaaaa ccaatattct ttatttttcc ttcttttttc ttttcttcaa
     3781 taaacttaaa gctatcaaat tttttagcta cttcataatg tgacacgcct atattgtgta
     3841 aaagatagta gtcaaaatag tctacaccac atttttctaa ttgctcatta aaaatggttt
     3901 cttgctcttc ttttttcttt aaagccatta atggaagttt tgtagctata gtaaaactct
     3961 ctcttttgtg tctttttact agagattctc taagtgctat ctcacttttt cccatgtgat
     4021 acacataggc tgtatcaaaa tatgtaaatc ccctttctaa aaaagtgtct accattttat
     4081 ttaaatgttc catatcaata gttgtaacat cattttcatc ttttattggt aaacgcatta
     4141 atccaaatcc taattttttt gcctccattg ttttacttcc ccctttaaaa cttgtttgtt
     4201 ttttctctct actttaacta ttcttacaag tatataattc ttgtataatt atactttact
     4261 acttggagtt aacttcaagt caatagattt ttttattttt aagacaaata ttataaaaat
     4321 acagttcttt aatattaatc cattgcatgt ttctttcacc ctccaattct caaacttcac
     4381 aaactccaac atgcaaagaa atatatactt taagaaattc agtccattaa tattactaca
     4441 aatacttttt agatatgttt aaaaagaaat ttcacctaaa atatttataa aagatttaga
     4501 aataggtctt gtgtctattt taaaatatat aaaacagtgg taaagactta tttataagat
     4561 aaaaaatgaa tagtgtatac cctttttaaa aagttttttc ctttttatat ttgtgataca
     4621 atgttaaata taaaaaggaa aattttgtat taaagaaatc ttatttgttg attttgtaac
     4681 ttcaaactca gtatcaattt gattttaaga ttatatttta ttaagtatgc taaataacaa
     4741 atttttaaag attgtgaggt aaaaatatga ataataaaaa tttagctata ttaatatcag
     4801 gtatgatgtt tttttctagt tcttcagcaa tttttgcaga caatactaca aagcaagaaa
     4861 gattaatagg taaaacaaga tatgaaacag cagtagaagt aagtaaattg ggatgggttc
     4921 aatcaaaaac agccattata gttaatggta attctattca aagtgcgctt tgtgcaaatc
     4981 catttgctaa gctaaagaat gcacctatac ttttagtaaa caacaacagt attgaaaatt
     5041 caactaaggc tgagcttaaa aggcttggtg tagataatgt gtacattgta gatagtggaa
     5101 atagcatttc ttctaaagta gaaaatgaaa taaaatcact taacataaaa ataaataaaa
     5161 tagtaggaaa caatatatat aaaatgtcta caaatgtact taaagaaata gataagataa
     5221 aaaaggtaga aaatgttgca gttgtaaatg gactaaaggg attatctgat gcagttagta
     5281 tagcttctcc agcagcaata cataatatgc ctataatatt agtatcagat gaaaacagca
     5341 atgatagtag tataaatttc ttaaacaata ggtctataaa aaaatcttac atagttggag
     5401 gaaataagat tttatcagac aatacagtaa agaaatatcc aaacagagaa agaatcagtg
     5461 gttcagatag aaacaatact aatgcaaaaa ttattgataa attttataaa gataaaaaaa
     5521 taaataatgt atttgtagct aaagatggta ttaaccaaga aaatgcatta gtagattcat
     5581 tatcaatagg agtattagct gctaaagaaa attctcctat aatgatatct aatggtagcc
     5641 ttgtaaatag tcaaaaggaa tttattaaaa ataaagatat agatgaaata acacaagtag
     5701 gaggaggata taatcaaaaa gcatttgaag atattttaaa actaaaaaat gtgaacataa
     5761 gaaataaaac taaaaaaata tctttaatac aggctaaaga aatattaaaa caagctcttc
     5821 caggaagcga tattttagtt tgttattatg atgaagatga taatgaatat gactgcaaag
     5881 ttataaagga taatatccta tatgagatag aaattgatgc ctactctggt aaaataatag
     5941 aaattgaaaa agatgatgac ttaaatgata ataatttagg tacacaaaat atatcattgg
     6001 ataaagctaa gtctatagca ttagatgctg ttcctaatgg taatttaatc tattgtaaat
     6061 atgatgctga agataacgaa tatgaggcta aagtaataaa aaataagaaa acatatgaat
     6121 tagagataag tgcatttgat ggaagaattg tagatattga catggacaat gattctgatg
     6181 atgactttga tgataatgat aagaataact ctaatactaa tgacaacagt tctattatta
     6241 gtgctgaaaa agctaaatca ataatgctag ataaagtgcc tggtgggaaa atagtaaaat
     6301 ttaagtatga tgaagatgac aaagaatatg aaggtgaaat aataaaagga aaaattgaat
     6361 atgagataac aattaatgca attactggta aaatattaga atttgaaata gatgattaat
     6421 aaaaaatata tattctatta attattacaa gcaataaagc cataaatatt gaatttttca
     6481 atatttatgg ctttatttta ttttttttaa tttaaatcta cattatgcca ttatatatct
     6541 atgcactctt ttaaattaaa atcttgtcca tcatcccaaa cctaataaag ttagatttta
     6601 aacaatatat tgtgatataa gtaatttgtg tagaattatt gcgtgattta ctcatgaaga
     6661 aacaataagg ttaatatata atttgactat attaagacga aaggagacat atacaatgaa
     6721 tacaatatta aaagtaaata acataactaa aacttataat aaagggcaac agccgagttt
     6781 agataaggta tcctttaatg tatcagaagg tgaatttata ggtataatgg gagcttctgg
     6841 ctctgggaaa acaactctgc ttaatgtatt atctacaatt gataaaccaa caagtggaga
     6901 tattttcata aataatataa atttgaaatc gcttaatgac acttgtgctg ctgattttcg
     6961 taaggataat ttaggcttta tctttcaaga gtatttttta ctagatagtc ttacaatttt
     7021 cgagaatatt tcagtacctc ttacacttca gaaactcaat ccagataaaa tagaaaacat
     7081 ggtaaagagt ctagctgaac gatttgacat agcttctcaa ttaaataaat acccaaatga
     7141 attatctggt gggcaaaggc agagagcttc agcagttaga gcaatagtaa aacatcctag
     7201 tattctcttt gcagatgaac caacaggagc tttggattct aattcagcta ctgaactact
     7261 ccactcacta caggaagcaa atgaggagtt gaaaacaaca attttaatgg taacacatga
     7321 tgcctatgca gcaagttttg caaatagaat tttgattttt aaagatggtc atatcattaa
     7381 agaattaaat aaacaaccga ataatagaca tacattttat gagttgatta ttcaagaaat
     7441 tgcaaaattg gatactaaaa gataggagga taatttaatg aacatttttt atatggcaat
     7501 aaagaatctt aaaaaaaact tttcattcta ttctctatat ctactttcag tctcttttgt
     7561 tattactgta tttttcgcat ttacttcatt ctctgttaat aagataatgc tagaaaaaat
     7621 ttcaactgat ggtagagttg aatctatgtg taatgttatt tcgatatttt tgatggcatt
     7681 tgtcatcttc tatatggcat actcaaatcg attcttcttg agacgtcgta caaaagaatt
     7741 aggcatatat gctttacttg gctatcgtaa gtttacaata ttatcgctac ttacctttga
     7801 aaatgtatta atttgtagtg gagcttttat tactggattg gtattaggag cattgtcaca
     7861 taagggtatt gtctttggaa ttacagcact acttaattta tcaattgata attcacagat
     7921 accatttttt aatataaatg caataaacaa aacaattatc tttatagcat tagttgtttt
     7981 agtcttagct atatcaaatg gtaaatttct ttataagact tcacttgtaa aattgataag
     8041 acatgaaaaa agtgcagaaa aaaaattaaa gttcagaaaa attccagcct ttatgggatt
     8101 tattatgatt ttaagtgggt attgtttatc actagatatt cttcgtggag ctaaatcagt
     8161 atgggtaagt attggatttt atcaaatggg cttgataact atgattatca ttgtaattgg
     8221 tacagttctc tttatcaatt cctttcttcc atatattatg caaaagagta aaaaaaataa
     8281 acgtactttc tacacagata tgaagataat agctattcca aactttatct atcgtatacg
     8341 ttcaaactct aaaactctaa ttatgcttac attactctct gctactactt tgacagtttc
     8401 aagtgttatg gcactgtcct tatactaccc tattgcatct gtttcgcgta ttgcaccttc
     8461 agagatagaa tttagagtag aaaaaaataa acaaatagaa gatgtaaaac aaattgtcag
     8521 tcaatatacg tctaatacag atgatattat ttttacacag acaaatattt ataaggtaaa
     8581 atcttcatca aaagaccttc catttgaata tgaaattggt acatctaaga ataatttatc
     8641 taatagtagt tcttctaaag aagttatttc aagagaatca ggatttgaat gtatatcttt
     8701 ttctgattat tatactttat taaaggcaca aaatcgaaca aagctactaa aacaacttac
     8761 ccctttaagg gataatgaat gtattttaat gaaatatcaa ccaaataatg atggatatag
     8821 tgaaaatgga acgatttatc cattacttat taatggtaaa gaaacacctg tagctgtaag
     8881 ggaaacatca ttaaacaatg ctatttcttt tgccaatagc gttggaacac ttatcgttaa
     8941 tgatagtata tatgagcaaa tagcacaaaa taaattacca atgacaagca ttttaagtat
     9001 taatggtaaa tcaattaaaa ataatgaaaa tttatatagt aatatttcag attatttaga
     9061 tggaagccct tatttacaag gatattctca caggataaat gatttattat caattaatag
     9121 ttctacattt ctgttaatcg gatttcttgt ggtattattc tttattgctg ttggaagtat
     9181 tttatacttt aacaatatat cagcaatttc agattcaaca tctgattata agattttaca
     9241 gaaaatgggt tatgataata tacaaattaa aaaaattatt aaaaaacaag tattgccatt
     9301 ttttaatatt ccgtttttac ttggtatatt ggactgtata tttgctacct tggtttataa
     9361 gactgctttg atgcagaatt tacttggaaa ttcattttca caatacatac ctgtgattat
     9421 tgcaattttc ttaacttctg tagtgtattt gatatattat atacttacta ttaaatcctg
     9481 ttgtaaaatt gtattaaaaa attaatattt accaaatcgt caagcttaga ggtgtgacga
     9541 tttggtatac ttcatttata agtatttgat ataatttaaa attatagatt cttaggtttg
     9601 atttagtcat aaaattaata taattaactc ataaggaggt gtataagatt gacaaatttt
     9661 aatttaaaaa tactattttt aggaaactac attctatcac ttctcttcat gggaatatct
     9721 acaagcactg aaacttatat gaatatccaa agcattattc ccttaataat ttcaacatta
     9781 ttactcatag acatcacata tataacctct tgtaatgtcc gagaaaataa cattatttca
     9841 ttattttgtg gattactagc actagacagt tggtatttac tcttatcatt tgtaaccaca
     9901 ccaataggca ctattatttt taaaatatta agcacagtta tagtgtatgt ctctattaaa
     9961 tttatatttt tatttttgtt tcaaggtagt ggatacaatt tccgtaagat aacagatatt
    10021 ttattgctta tcacctgcat tggtgcaatt gtaggagcat ttatttctaa cagaacgtat
    10081 gcttgtatgt acggaataca gtttgttgtt agtataataa gctttttata tattattttt
    10141 tatcatttga aaagggttgt ttttgtcata aaaagcgaaa aaaagcctat atcgatttca
    10201 gtatttataa caattatagg attccttact tattattttt ttacactaag aatatcaaat
    10261 catattggta attttggaat ctatctaact gtattggtat tttttatgag tattcatggt
    10321 attgcactta aagaaaatga tagttttcct atttcaacag tttttaacat aaaacaaagg
    10381 gctatatttc tttttataag tattttagta attagcttgg tcgtactatt tgccaatggg
    10441 aatttttcta taatattaat agctataaat gttttattta tgcttatttt cctctgtaac
    10501 attattttgg agtttaacct tagagatgaa aaaaataaag tagttaaaaa tagcaagtat
    10561 acttttgcat tagaaaaatt acaacaagaa gaacaactaa aaacagagtt ttctaatttt
    10621 ttacatgatg acatattaca gaacctgctt tcaataaaaa acatgatgag taagtcatat
    10681 cgcccagaag ttcaagatat tatatatgaa acacttaaca atctaaatac atgtataaga
    10741 aatcaaatgc aagactatca tcctgttatt ttaaaaaatt taacagtaaa agaaaattat
    10801 cagaatttaa ttgaaagtgt ttcttcatcc tttccacaaa aaaacatttg tgtttcattt
    10861 aagtgttctg atactttatt cttagttgaa ccatatgata tattgatata tcgactgatt
    10921 aaagaattat tgacaaacgt gtataaacat tcaaatggta gccatgcatg gatagcttta
    10981 gcactagaaa aaaatataat aaaattaaat attaatgata atggtgtgaa aaatacatct
    11041 tttaaaacac ctttagatat aactaaaatt acttttgata taataaaaca caaagggatt
    11101 atatcaatta aagaacaagt agaaaatcta ggaggaacga taagtatttc tgataatata
    11161 cctcatggag tttgtgtaca gattacaatc cctatgaaag gagatggttc ttataaatat
    11221 tttgttagtt gatgaccata tgctttttgc taaaagcttg tcaattgcac tagaagaata
    11281 cgaagaaatc gaacagtttt attccacaca ggacatccat aatctttcag aaatgattca
    11341 agataaaaaa atagacattg tcttaatgga tataaacctt agaggcttaa gtgaatgtga
    11401 tggcttaaaa gtagcttata atttattaga gactaattca actattaaaa taataatctt
    11461 gactggatac gacctcccag tttataaaca tgaggctaaa aaaataggta tctatggatt
    11521 cctaaataag aatattagcc ctgatagttt aattgattca ttattaaaag tctatcaagg
    11581 ggtaacctgt tttccatctg aaaatactga agtaattatt gaagatttaa ctaatatgga
    11641 gaaaagaatt ttacagctta tctgtagtgg taaaaaaaga aaaataattg ctgatgaatt
    11701 gtatataagt gaaagaacat tatcaaatca tttacaacac atatatgaca agctagatgt
    11761 ttcatcatca gttgaggcta ttacaaaagc cattaaaatg ggttatattt ctccagtata
    11821 ttaaaaaagt gagtgtctca aaatgaactt tctagctcat aaggcactct cctttgtatt
    11881 aaatcaaata tattttcatc atttaaacaa ttaagaagag gcttctctct attttgagac
    11941 aactccattt taatacacta tttatattta tatataaaca tattttaaac aactaaaaag
    12001 caactaatat ctcaatacat atcttcataa cacatatcac cataattaca agcagataaa
    12061 gaactctcct catttaaata cgactcaaat tcatacccaa acagaattgt tggttctata
    12121 tatatttcca tatctgtata tctccatctt cttacctttg cctctataac cttgtaaaaa
    12181 tcttctaaac tatacccttc ttttaatctt ttatctataa gtgccttagt tatactcaaa
    12241 tcagctttaa aattttttcc tgattcacta tttaattttc ttataataat ttcatgtatg
    12301 ctttctggat ttttataagt agaatcttca gtcaaatctt catccaaatt ttcaaaggta
    12361 tttttattta aattcttttt atttaaatta ttttttaata attctttttt ctttgtctca
    12421 ttttctgttt catacccagt atgaattatc aaatcataat ctttgtctat actctcttga
    12481 tttatacggc tgtatgtgtt ggtattactg ttcttgaaca actcatctct atgtttatat
    12541 ttcattgttt tttggttatt gttaatagtt gcatcaatgt atttctctat atttttgaaa
    12601 atatcactat tctttgttac aatttctgca ttactatttt cagaacaaac ataactatat
    12661 acagaacaac aatttccctt tactccccta cttattaatc tcaatatacc taaatctata
    12721 aattcattta caagtctact tattgtagat atagatacat caaggtcaga tgcagctttc
    12781 cttactgtca tcataaattg tcctcttggc agagttcctt ttaatctttg tatattttct
    12841 tttctcatta catagtgatt aaaccttatt ctatttacat ctcttttttc attaaaatat
    12901 aatactgctg aatttaattg ataaaaacta ttactcatat tcccactcct ccaatttaaa
    12961 tagataattt gttgtaaagt ttttagattt ttaaataact t
//
LOCUS       DAMJWP010000091        12888 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.095, whole genome shotgun sequence.
ACCESSION   DAMJWP010000091 DAMJWP010000000
VERSION     DAMJWP010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12888
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.095"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            286..987
                     /locus_tag="PC700_002131"
     CDS             286..987
                     /locus_tag="PC700_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDF2581440.1"
                     /translation="MNNSFLDKLIETKELKNSLYNVLKHNFLYHSNKIEGSTFTTEAL
                     ALLLDKNVVTGRHTLDDVQETVNSSYVFDTVIDSLKEKITHNFLRNLHSSLIFNTTLH
                     SRGMAGIYKTIPNMILGTDVSIAQPFEVEPKLDELIEWYYSQSEVSIKVIAEFHYRFE
                     LIHPFQDGNGRIGRFVMLKQMLENNLPIKIVSWDSEDLYRNSLNSCSLGNYVPLIEYL
                     SSLEDFREVYKMLWK"
     gene            1891..2541
                     /locus_tag="PC700_002132"
     CDS             1891..2541
                     /locus_tag="PC700_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2581441.1"
                     /translation="MKVKSNLDEILYHKIIESLIRGEYSVGQKILLNDLCEKFEVSRT
                     PVVQAVKMLNKDGVLTIMTNGKVYVPEYEYDMVKQVCETRTLIETYALEKMMQEEEEV
                     FQQKLDVIKRYSDKCEEYYRQEKSVELALADLELHKAIVEGANNEILNDVYVGIQGRF
                     IVVNYLIRPLKNRNYEGTVQDHFEILKFIEDRDTKKAVDKLRNHIQGTIKRFCEDE"
     gene            2819..4885
                     /locus_tag="PC700_002133"
     CDS             2819..4885
                     /locus_tag="PC700_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HDF2581442.1"
                     /translation="MKEVVESLKKITKERIENTMKKLFTSETFLEYQFISDCQISPDG
                     GYTAFIVKKADIKENGYTSQVYILNNKTGELKQITSINSVGAYVWEDENTILFPALRN
                     EKVKEAVKNGKQCMSYYALSLNGGEAEELFRLPIKGGKLNPIGKGLYAVIDSYDNDRP
                     IVEGLPEEEQQKKINAYNKRHYEHFKEIPYAVNGEGYISRKRKRLYIYDSNINKLKAI
                     TAPMFNVVGMKISDGKILYIGQEFKDVKGLKNGVYVFDTNTNTNICILEKDKYIIKGF
                     ELYQNQVILNLTDALSYGNGENGDFYTIDIDTKEMKLLSGHQHHCIGNTVTSDVKMGA
                     GQTTKVDGEYIYYTSTVDMDCIIERIHIPTGKQEKVTQTGSVDFIDVKDGNIVCVGCL
                     GNGLPEVYTVENGTLCKKTHLNDHILEEYKISVPEYIESKGSSKWEIQGYVYKPVDYE
                     VGKKYPAVLAIHGGPRLTYGPYFMHEIQVFTSAGYFVFFCNPRGSEGRGNAFADIRKQ
                     FGDIDYIDFMEFTDTVLEKYTDIDKTKLAVEGGSYGGFMTNWIIGHTNRFAVACAQRS
                     IANWSSMEGTTDIGYYFCKGQTGASHMENHELQWKQSPLAYADKCVTPTLFLHGEKDY
                     RCYMQEAFQMFSALKIHGCPTKLCLFEGENHELSRSGRPKQKLQRLVEMLDWFSVYIK
                     KDNIGQ"
     gene            4902..6857
                     /locus_tag="PC700_002134"
     CDS             4902..6857
                     /locus_tag="PC700_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HDF2581443.1"
                     /translation="MEKIKIESSDIFEYIFPHDISCSPDGKHIAYIISNINEEKDCYE
                     HDLYVMDIKTEKQIHMTQTKDVTSFSWISNTELLFTSKRNKPKAGTTDFYTISIEGGE
                     AKEAFSIPKACSVPVSLGNKLWLLTTKNPTDSKKSEPDRAVEGVDYWTFTDKPFIRDR
                     ENFSQRRRVTLELYQEGENITKAITPKFCEVAGIDVSSDKNRILYTGQIYEDCATPFS
                     GLWEYHIDSGETKELVPQGKYQISLAKYIGKDKVMLQASTLERSITQNHDIFILDLST
                     SEINMIASPDGMYATLLDVDAVYGGGRSNKVIGDKFIGARICRTMTEFNEFDTKTGNI
                     RIITKVDAFTSFDIYDNTMYTVMLKDYELAEIYSIDMTTGTMKKMTAFSKPYLDTHKV
                     SLPEKLTFVAKNKEEVDGFVIPPIDAKEGEKYPAVLFIHGGPKWAYGYMFTHLKQCVT
                     SKGMYVIYCNPHGGDGYGEKFLEMVERWGYVDYEHLMEFVDTCIEKYPGIDADRLGVA
                     GGSYGGYMTNWIIGHTDRFKAAVSQRGISNLITASLIIDFGDRIMKQTCGDKTPWNHE
                     EVLWNHSPIKYVKNVKTPTLFLHSDRDYRCFMGDTFQMFTALKQLGVDTEMYLFHGDT
                     HGLSRNGRPSNRIARANAIVDWFERYL"
     gene            6873..8063
                     /locus_tag="PC700_002135"
     CDS             6873..8063
                     /locus_tag="PC700_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDF2581444.1"
                     /translation="MSDMLLWFSVVLVLFAIGDLIASKTKAKVSAVFVTLLLFLILFV
                     TKAIPADIIEKAGMTAAASWSVPMIMFSMGTMLNVKQFIDEWRTVLTAWLGIVAVIVC
                     VSLCIPLFGKSTVLTSIPVINGALPATTIMTQAALEKGLTLAAATATVVFAIQKFIGT
                     PIASRAALQEANRLLVEYHEAKSKGIDLANVDTDKKEAENTGTKVKQAFCEKYDKYYS
                     TNVCIFFIALFSYLGYELSEIIHVNYSIVCLVVGVIVTRIGIVPKDILEKGKIKGFIN
                     MVVFAAVIPSLAKVSLTDLISLFVPIVGMFAASIIGIFLMMKVLPGWKIIGSKPLAFG
                     VGFCQMLGFPTTYLISNEVCNAVGETEEERAYLMSKIMPKLVVGGMACMISIVVAGLM
                     VPML"
     gene            complement(8911..9094)
                     /locus_tag="PC700_002136"
                     /pseudo
     CDS             complement(8911..9094)
                     /locus_tag="PC700_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427897.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9197..9571
                     /locus_tag="PC700_002137"
     CDS             9197..9571
                     /locus_tag="PC700_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2581445.1"
                     /translation="MKVLISNTSDKPLYQQIKEQIKEAIFTEELKEGDAMPSIRNFAN
                     DLKVSILTIRRVYEELENEGFLTRQVGIGTFVSTGNLEILRDSKRRIVEQKMADMIKD
                     AKALGITQIELQEMMGILYEED"
     gene            9573..10430
                     /locus_tag="PC700_002138"
     CDS             9573..10430
                     /locus_tag="PC700_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2581446.1"
                     /translation="MENILEVYNLNKTYTNFSLSNISFVLPEDCITGFIGVNGAGKTT
                     TIRSILDVVKRDSGKIKIFGKDFDGNEKELKNRIGVVFDDSYFYEELSMSEMKSIIAP
                     SYDNWCEEDYQQNMKRFNLNPKQKIDTLSKGMKMKFALALALSHKAEFLIMDEPTSGL
                     DPLIRSQILDILTEYMKKCGRGVFFSTHITSDLDKIADMIVMIDNGRIILEREKDTLL
                     DEHRKVKGDIQFLNDEIKKLFLNLEITGYGFIGITSKINEVQKNMPDILIERPTIEDI
                     MLAYIERRE"
     gene            10433..11071
                     /locus_tag="PC700_002139"
     CDS             10433..11071
                     /locus_tag="PC700_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HDF2581447.1"
                     /translation="MLIHLVKKDILIVKKYVLLILFIAIGIPLFILWRLPPFAQILGF
                     VLSIIFSEFMLCQYLLLKESQYSKASVLLCSTPYPRRGLVQSKYVLFAMVFIYCTLVY
                     WIENLLIPQIGTFDLTYILIVLLISSIIYGVYMPIQYKLGYETTKFFFVMIIMASSFV
                     LPLTIAHSNFEIPFIYQYPSMIVNIVLFLLSLIILLASMFISIKIFSNKELV"
     gene            12045..12779
                     /locus_tag="PC700_002140"
     CDS             12045..12779
                     /locus_tag="PC700_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2581448.1"
                     /translation="MNLAKGLDVLKISSNVFGEDKVMYIPVIYTEDDATLIDTGLPGQ
                     GDLIIDALNKSNTSFDRLKNIIITHHDIDHIGNINYLREKSKNNIKVYAYKSEVSYIT
                     GEETPFKLYMLEQMVDKIDDKMLSMLNVMVLGFKSSYTKVDVSLDNHEKLNLGEEIEV
                     IHTGGHTRGHICLYLKESKVLIAGDLLQVENGELTPVDVMHSNKQELKDAIKNISNYD
                     IETIVFSHGGLYQRDIIGTLKNLIIE"
BASE COUNT         4844 a         1467 c         2188 g         4389 t
ORIGIN      
        1 aaatgaactc taagaagaga taaaaatgcg ttttatatgt ttttagatta atatgaaata
       61 tcacaatgaa atgttgtaca aagtgatata taataattat atgaactata aaattgacta
      121 aacttattga ctaataaatt agatgaaaag ttgagactta ctgaaaataa atacaatgaa
      181 atatatacta caagtattaa attaaatctg aactttacat actattagaa taagtattat
      241 aagattataa tgatttaaaa atattaaaat aattgaggtg ataacatgaa caattccttt
      301 ttagataaat tgattgagac aaaagaactt aaaaattcac tttataatgt gttaaaacac
      361 aattttcttt atcattctaa taagatagag ggcagtactt ttactactga agcattagca
      421 cttttactcg ataagaatgt tgttacagga agacatactt tagatgatgt acaagaaact
      481 gttaattcta gttatgtatt tgatacagta attgactcac tgaaagagaa aataacacat
      541 aatttcttaa ggaatctaca ttcaagttta atcttcaata caaccctaca ttccagaggg
      601 atggctggta tatataaaac tatacccaat atgatactag gaactgatgt aagcatagca
      661 caaccatttg aagttgagcc aaagcttgat gaattaattg aatggtacta tagtcagagt
      721 gaagtgtcta ttaaagttat agctgaattt cattatagat ttgaattgat acatccattt
      781 caagatggta atggtcgcat aggtagattt gttatgctaa aacagatgtt agaaaataat
      841 cttcctataa aaattgtatc ttgggatagt gaggacttgt atagaaactc cttaaattct
      901 tgtagtttag gtaattatgt ccctttaatt gaatatttat caagtttaga agattttaga
      961 gaagtatata aaatgttatg gaaataattg tttgtagcaa aaaaagtttt tatataaata
     1021 tatttttaac tatgctattt aacgttgatt tcttagttat tcttagcttg gactataccc
     1081 actattttaa ctatataaaa tagtggatat agtttaacta tctctttata actattttta
     1141 tgtatcttag ttgtttctta atattgggac agttcataaa actaaaagtg atttttaatt
     1201 ttatcattta taatttacca ttacatcaga aaaatgctta tcattagaat tttcataaaa
     1261 ggtacctatt ttcttcaatt aactaaatca attctattag tcttaatctt tcgtatttca
     1321 aattgataat acaagaaaat aaaaaataaa aaattttaaa ttttttattt attgcgaaaa
     1381 aaataaaaag gtgctatact ttttgtaaca tattattcaa attattaaga gatagtaaat
     1441 atttatagtt taatttgtag tacactagca tgatttgcag aacaatgaat taatctatag
     1501 tataatagtt taatttgaag tacaacgatt taatctatag tacactagca tgatttgaag
     1561 tataacgatt taatctatag tacaataact tgatttgcaa tacaataatt taatctatag
     1621 tataatagca tgatttgaag tataacgatt taatctatag tacaataact tgatttgaag
     1681 tacaacgatt taatctatag tacaataact tgatttgcaa tacaatgatt taatctatag
     1741 tataatagca tgatttacag tacaataatt taaattgtag tacaaaagta ctatcaaata
     1801 agaatttcta ctttaaaaat attactaaaa gtaattaaaa tccaataatt caatataata
     1861 tggcaaaaga atagaaataa ggtgagagta gtgaaagtaa agtcaaattt agatgaaatt
     1921 ttatatcata agattattga aagtcttatt cgtggtgaat actctgtagg tcaaaaaata
     1981 ttactaaatg acctttgtga aaaatttgaa gtaagcagaa caccagttgt acaagcagta
     2041 aaaatgctaa ataaagatgg agtgcttact attatgacaa atggtaaagt gtacgtacca
     2101 gagtatgaat atgatatggt aaaacaagtt tgtgaaacga gaacactgat tgaaacatat
     2161 gctttagaaa aaatgatgca agaagaggaa gaggtttttc aacaaaagct agatgtaata
     2221 aaaagatact ctgataaatg cgaggagtac tatcgtcaag aaaaatctgt agaacttgca
     2281 ctagcagatt tggaattaca taaagccatt gtagaagggg caaataatga gattttaaat
     2341 gatgtatatg ttggtataca aggaagattt atagtagtga actatttaat tcgaccatta
     2401 aaaaatcgta attatgaagg aacagtacaa gaccattttg aaatattaaa attcattgaa
     2461 gacagagata caaaaaaagc agtagataaa ctaagaaatc atattcaagg aactattaaa
     2521 agattctgtg aagatgaata ataagttttg aagttactat atacttatct attaagttgc
     2581 taaatgtagt ctaagtataa tctaattata gtttaaacat aatctaaata taatctaaat
     2641 atagtgtaaa taagtctaag tatgaattaa atatagatta agtatgaatt aagcatggat
     2701 taaatatact ttaaattaca aatattacaa ggaagccaga aggcttccta aaaaaataac
     2761 ctttataaaa ttttttattt taaatttaaa attttttatt ttaaatttaa aataaaaaat
     2821 gaaagaggtg gtagagagtt tgaaaaaaat tactaaagaa aggatagaaa atactatgaa
     2881 aaagctattt acgagcgaaa catttttgga atatcaattt ataagtgatt gccaaatatc
     2941 accagatgga ggatatacag catttattgt aaaaaaagct gatatcaaag agaatggtta
     3001 cacaagccaa gtttatatat taaacaataa aactggcgaa ttaaaacaaa taacgtctat
     3061 aaactctgtt ggagcatatg tctgggaaga tgaaaatact atcttgttcc ctgctttgcg
     3121 aaatgaaaaa gtaaaagaag ctgtcaaaaa cggtaagcaa tgcatgagtt actatgcttt
     3181 atctttaaat ggtggagaag cagaagaact ttttagactc cctatcaaag gtggtaaatt
     3241 gaatcctata ggaaaaggtt tgtatgcagt tattgattct tatgacaatg accgacctat
     3301 tgtagaggga ttgccagaag aagaacaaca gaaaaaaata aatgcttata acaaaagaca
     3361 ttatgaacat ttcaaagaaa ttccttatgc tgtcaatggg gagggatata ttagcagaaa
     3421 aagaaaacgc ctatatatat acgacagtaa tataaataag ttgaaggcaa ttacagcacc
     3481 tatgtttaat gttgtaggta tgaaaataag tgatggaaaa atcttatata tagggcaaga
     3541 gtttaaagat gtaaaaggtt tgaaaaatgg ggtctatgta tttgatacaa acacaaacac
     3601 aaatatctgt atattagaaa aagataagta tattataaaa ggttttgaat tatatcaaaa
     3661 tcaagtgata ctaaacctaa ctgatgcttt atcttatgga aatggagaaa atggagattt
     3721 ttatactatt gatattgata cgaaagaaat gaaacttctt tcagggcatc aacatcattg
     3781 tattggaaat acagtaacat ctgatgttaa aatgggtgct ggtcagacaa caaaggtaga
     3841 tggagaatat atttattaca cttctacagt agatatggac tgcattattg aaagaataca
     3901 tattccaaca ggaaaacagg aaaaagtaac acagacaggt tcagttgatt ttatagatgt
     3961 aaaagatggc aatattgtat gtgtgggttg tttaggaaat ggattaccag aagtttatac
     4021 agtagagaat ggaacattgt gtaagaaaac acatttaaat gaccatattt tagaggaata
     4081 taaaatttct gtgccagaat acatagagtc aaaaggaagt tctaaatggg aaatacaagg
     4141 atacgtatat aaaccagtag attatgaggt ggggaaaaaa tatccagctg tattggcgat
     4201 tcatggagga ccaagattga catatggacc atattttatg catgagatac aggtatttac
     4261 aagtgcaggt tactttgtat tcttctgtaa cccaaggggg tctgaaggtc gtggcaatgc
     4321 ttttgcagat attaggaaac agtttggaga tattgattat atagatttta tggaatttac
     4381 agatactgta cttgaaaaat atacagacat tgataaaaca aaattggctg ttgaaggtgg
     4441 aagttatggt ggatttatga cgaattggat aattggtcat acaaaccgtt ttgctgttgc
     4501 ttgtgctcag cgttctattg caaactggag tagcatggag ggtacaacag acattggtta
     4561 ttatttctgc aaaggacaaa caggtgcttc tcatatggaa aaccacgagc ttcaatggaa
     4621 acagtcacct cttgcttatg ctgataaatg tgttacaccg actttatttt tacatggtga
     4681 aaaagactat agatgctata tgcaagaagc attccagatg ttttcagcat tgaaaattca
     4741 tgggtgtcct acaaaacttt gtctatttga aggggaaaac catgaactgt ctcgttctgg
     4801 tagaccaaaa caaaagctac aaagattagt tgaaatgcta gattggtttt ctgtgtacat
     4861 aaaaaaagat aacataggac agtagaaagg agaatataaa aatggaaaaa ataaagattg
     4921 aaagttcaga tatttttgaa tatatattcc ctcatgatat ttcttgttca cctgatggaa
     4981 agcatattgc ttatattata tcaaatatca acgaggaaaa agactgctat gaacatgact
     5041 tatatgtgat ggacatcaaa acagaaaaac aaatacatat gactcaaaca aaagatgtaa
     5101 catcatttag ttggatttct aatacagaac tactttttac atctaaaaga aataagccaa
     5161 aagcaggtac aacagatttt tacactattt ctatagaagg aggagaagca aaagaagcat
     5221 tttctattcc aaaagcttgt agcgtacctg tatcacttgg aaataaattg tggttattga
     5281 caacaaaaaa tccaacggat tctaaaaaat cagaaccaga tagggcagta gagggtgtag
     5341 attattggac atttacagat aaacctttta taagagatag agaaaatttt tctcaaagaa
     5401 gacgtgtaac attagaatta tatcaagaag gtgaaaatat aacaaaagcc attacaccaa
     5461 aattttgtga agtagcaggt atagacgttt cttctgataa aaacagaatt ttatatactg
     5521 gtcagattta tgaagattgt gcaactcctt tttctggact ttgggagtat catatagaca
     5581 gtggtgaaac caaagaactt gtaccacaag gaaagtacca aataagtttg gcaaaatata
     5641 ttggcaaaga taaagtaatg ttgcaagctt caacattaga acgttctatc acacagaatc
     5701 atgatatttt tatacttgat ttgagtacta gtgaaataaa tatgattgct agtccagatg
     5761 gtatgtatgc tacattactg gatgtagatg cagtatatgg tggtggacgt agtaataaag
     5821 ttataggaga taaatttatt ggtgcaagaa tttgcagaac tatgacagaa tttaatgagt
     5881 ttgatacaaa gacaggtaat atacgtatca tcacaaaagt agatgctttt acatcttttg
     5941 atatttacga caacaccatg tacacagtaa tgttgaaaga ttatgagtta gcagaaatat
     6001 atagcattga tatgactaca ggtacaatga aaaaaatgac tgctttttct aaaccatatt
     6061 tagatactca taaggtttca ttacctgaaa aattgacgtt tgtagcaaaa aataaagaag
     6121 aagttgatgg ttttgttata ccacctatag atgctaaaga aggcgaaaaa tatcctgctg
     6181 tattgttcat tcatggtgga cctaaatggg catatggcta tatgtttaca cacttgaagc
     6241 agtgtgtaac atcaaaggga atgtatgtga tttattgtaa ccctcatggt ggtgacggtt
     6301 atggtgaaaa attcttagaa atggtagaaa gatgggggta tgttgactat gagcatctta
     6361 tggaatttgt agatacttgt attgaaaaat atcctgggat tgatgcagac cgcttagggg
     6421 ttgcaggggg aagttacggt ggttatatga caaactggat aattggccat acagaccgtt
     6481 tcaaagcagc agtttctcaa agaggtatta gtaacttgat tacagcttct ttaattattg
     6541 actttgggga tagaattatg aagcagactt gtggagataa gacgccttgg aatcatgagg
     6601 aggttctttg gaatcattca cctattaaat atgtgaaaaa tgttaagaca ccaacattgt
     6661 tcttacatag tgatagggat tatagatgtt ttatggggga tactttccaa atgtttacag
     6721 cattaaaaca gcttggtgta gatacagaaa tgtatttgtt ccatggagat acacatggat
     6781 tgtctagaaa tggaagacca tctaatagaa ttgcacgagc aaatgctatt gtagattggt
     6841 ttgaaagata tttataataa gggggttatt ctatgagtga tatgttatta tggtttagtg
     6901 tcgtattagt tttatttgct attggggatt taattgcaag caaaacaaaa gcaaaagtat
     6961 cagctgtatt tgttacacta cttttatttt tgattctatt tgtcacaaaa gcaataccag
     7021 ctgatattat tgaaaaagca ggtatgaccg cagcagctag ttggagtgtt ccaatgatta
     7081 tgtttagtat gggaacaatg ttgaatgtta aacaatttat agacgaatgg agaacagtat
     7141 tgacagcatg gcttggaatt gtagcagtta tagtctgtgt ctcattatgt attcctttgt
     7201 ttggaaaatc aacagtattg acaagcatac cagttataaa tggagcttta cctgctacta
     7261 ctattatgac acaagcagca ctagaaaaag gactgacact tgcagcagct acagcaacag
     7321 ttgtatttgc aattcagaag tttattggta caccaatagc ttcaagagca gcgttacaag
     7381 aggcaaaccg tcttttagta gaatatcatg aagcaaaatc aaaaggcatt gatttagcaa
     7441 atgttgatac tgataagaaa gaagcagaga atacaggcac aaaagtaaaa caagctttct
     7501 gtgaaaaata cgataaatat tattctacaa atgtttgtat attttttata gcactattct
     7561 cttatctagg gtatgaactt tcagagatta ttcatgtaaa ttattctatt gtatgtcttg
     7621 ttgttggtgt tattgtaaca cgtattggta ttgtaccaaa agacattttg gaaaaaggaa
     7681 aaatcaaggg atttattaac atggttgtgt ttgcagctgt tattccttct ttagcaaaag
     7741 tttctttgac agatttaatt agtctttttg taccaattgt gggtatgttt gcagcttcta
     7801 tcattggtat attccttatg atgaaggtgt taccaggttg gaaaattatc ggttctaaac
     7861 ctcttgcttt tggagtaggg ttttgtcaga tgttgggatt cccaactaca tatttaattt
     7921 caaatgaagt gtgtaatgct gttggagaaa cggaagaaga aagagcatat ttgatgtcta
     7981 agattatgcc taagctggta gttggtggta tggcttgtat gatttctatt gtagtagctg
     8041 gtcttatggt accaatgttg tagataggtt tcatagttaa aaagtggtgt taatttatag
     8101 gagaagctaa aaagtagtat taatttataa aataatttta tattagttaa tttgttataa
     8161 gaatattttc tttttatgat tataattttg attttataag agagcagatt ttttatttgc
     8221 tatgctaata gttataattt gtttttattt gctaaaatgc aactttaatt tgatttatgt
     8281 ataatatagg gtatttaata atatttttga agaattttta taaaaaatta aaaagaggag
     8341 aaaaaaggga gtgccacaaa gtgaactttt tagttctaag gcactctttt ttgtacgaaa
     8401 tcgaatatat tttcaacgtt ttaaaagtga gaaagagact tatctctagt ttgggatagc
     8461 caaatttaat tattaaaatt attttttgtt ttatttatca aagagtattc gtgttttaga
     8521 agttagtatt ttttatttta tatatccaag gttttccgtt tctaatgttt tcttttttaa
     8581 aataattagg gtattcaatt atagtaaaag gtggatggta ttttggaatg atattattta
     8641 aagcacagta ttgaatgttg aaatcctcac attttagagt attatctttt gtaatcaaaa
     8701 aaatcttcta tacttatagc aaggttactt ctgcaaatat tccatgcatc ataatatcca
     8761 aatttaaatt tttttaatat agagtttacc tctagtctac actcttgaat aactctttcc
     8821 tctatccaaa ttcttataaa ctcatcccat ttattactat ctatttatta ctatctaaat
     8881 tttttagtat gtttgtaatt ggaaatggat ttattccttt atgtttttta taaatagttt
     8941 ataacttaat ttattattaa acaaatcatt catacagaat tactttttct tttagatatt
     9001 caattcttac ttttaataaa tatttaatac attctatctt atgtggtttt aaaaagtgtt
     9061 tatggctatc aataatctct aatatttcat tcatatttat tttgtcatag taatatttta
     9121 catcttttat attgacaaaa aagtgctcta agtgtatagt gtgtatataa taacatcaca
     9181 ctggaggtga taaaaaatga aagtgctcat ttctaacaca tcagataagc cattatacca
     9241 acaaattaaa gagcaaatta aagaagcaat atttactgaa gaactgaaag aaggggatgc
     9301 aatgccctca ataagaaatt ttgctaatga tttaaaagtt agtattttga ctattagacg
     9361 agtgtatgaa gaacttgaaa atgaaggttt tcttacacgc caagttggaa taggaacatt
     9421 tgtttcaact ggaaatttag aaatactaag agactcaaag aggcgtatag ttgaacaaaa
     9481 aatggcagat atgattaagg atgcaaaagc actaggaatt acacagatag agttacaaga
     9541 aatgatgggt attctatatg aggaggatta atatggaaaa tattttagaa gtttataact
     9601 taaataagac ttataccaat ttttcattat ctaatataag ctttgtatta ccagaagatt
     9661 gtataactgg atttataggt gttaatggag caggaaaaac aacaacaatt cgttcgatat
     9721 tggatgtcgt aaagcgtgat agtggaaaaa taaaaatctt tggtaaagat ttcgatggaa
     9781 atgagaagga attaaaaaat cgaattggag ttgtatttga tgattcatat ttctatgagg
     9841 agttgtcaat gtcagaaatg aaaagtatta ttgcaccttc atatgataat tggtgtgaag
     9901 aagattatca acaaaatatg aagcgtttta atcttaatcc aaaacaaaag attgatacac
     9961 tttcaaaagg aatgaaaatg aaatttgctt tagcattagc attatcgcat aaagctgagt
    10021 ttttaattat ggatgagcct acaagtggtt tagacccact aatccgtagt cagatacttg
    10081 acatactaac agaatatatg aagaaatgtg gtcgtggtgt tttcttttct actcatataa
    10141 catctgattt agacaaaata gcagatatga ttgttatgat tgacaatgga cgtattatat
    10201 tagagcgtga aaaagatact ctattggatg aacatagaaa agtaaaaggg gatattcaat
    10261 ttctaaatga tgaaatcaaa aaattatttt taaatttaga aattacaggt tatggtttta
    10321 tagggattac ctcaaaaatt aatgaagtgc aaaaaaatat gccagatatt ttaatagagc
    10381 gtcctacaat tgaagatatt atgcttgcat atattgaaag gagagagtaa ttatgttgat
    10441 tcatttagtt aaaaaagata ttctgattgt aaaaaaatat gtattgttga tattgtttat
    10501 tgcaatagga atcccattat ttatattatg gcgtttacct ccatttgcac aaatattggg
    10561 atttgtacta tcaattatat tttcagaatt tatgctgtgc cagtatctat tattaaagga
    10621 gagtcaatat tcaaaagcat ctgtattact ttgttcaact ccttatccta gacgtggctt
    10681 agttcaatct aagtatgttt tatttgcaat ggtatttatc tattgtacat tagtttactg
    10741 gattgaaaat ttacttattc cacaaatagg aacttttgac ttaacatata ttttgattgt
    10801 tttattaata agttctatta tttatggagt ttatatgcct attcaataca aattaggata
    10861 tgaaacaaca aaattctttt tcgtgatgat aatcatggct tcatcatttg tattaccatt
    10921 aactatagct cacagcaatt ttgaaattcc atttatatat cagtatccat ccatgatagt
    10981 gaatattgta ttgttccttt taagtttaat tatactgtta gcttcaatgt ttatttctat
    11041 taaaattttt agtaataagg agcttgtata aggtaagggg gaattttata tgccacattt
    11101 gccagattgg gctgttttac tttgtatctt acttccaata gtatataaag cttgtcagat
    11161 atttaaaagt aaaaaataga gtacatattt atattgccat ttttattgag tgaagttaga
    11221 tggtatgaat ttaaaaggtt acttgaaaat aaagttactt ggtgtgggag agtaacatca
    11281 taatattata gattatatgt ttcttgtgaa tcaatttgta ttttttaata gataatatcc
    11341 agtaatttta ttttccattg attattttca attatgagta tgattgtgtg tattgtatac
    11401 catcttttaa ggtgttttta aaaaattata tcgtcaaaat ataaatctaa ggaatcctat
    11461 aaataaagct tttcaatttt agaaaactaa taaaaagtag gaagttaata aaaagaagct
    11521 gtctcaaaat agagataagc ttctttttca ttgctgaaat gacgaaaata taattgattt
    11581 aatataaaaa agagtgcttt atgaactatt tattttgaac agtagataca cttgcactca
    11641 atttagttac tcctaagctt tatgaactaa aagtttattt tgaagcctat ttttctaaat
    11701 aatcatctat atacagtatt gcgtttcttt tatttattca taatttattt tcttattaaa
    11761 gtttttctat attactttac ttttaatttt atacgaatta tcaaaaataa aatagttatt
    11821 agaaaaaaat aaaagtaatt gcaaagattg caattactta cttctatatt aattactata
    11881 ccataaaata acaaaaaaat caagtcgagt aatccttcaa cttaagtgat atactaggta
    11941 ttaataattt ttaattaata aaatataact agaggaaaat ctacattttc tcaatttctt
    12001 taattaaatt gacctctaat taagtaatta taaggagaaa aattatgaat ttagcaaagg
    12061 gattagatgt tttaaaaatt tcatcaaatg tattcggtga agataaagtt atgtatattc
    12121 ctgtaatcta cacagaagat gatgcaacac ttattgacac aggtcttccg ggtcaaggtg
    12181 atttaataat tgatgctctt aataaaagta acacgagctt tgataggtta aaaaatataa
    12241 taattactca tcatgatata gaccatatag gaaacataaa ttatctaaga gaaaaatcta
    12301 agaataatat caaagtatat gcatacaaaa gtgaagtcag ttatataaca ggagaagaaa
    12361 ctccttttaa actttatatg ttggaacaaa tggtagataa aattgatgat aagatgctta
    12421 gtatgctaaa tgttatggta cttggattta aatcatcata tactaaagta gatgtgtcat
    12481 tagataacca tgaaaaattg aatctaggtg aggaaataga agttatacat acaggaggtc
    12541 acactagagg tcacatatgt ctttatttaa aagagtctaa agtacttatt gctggagatt
    12601 tgttacaggt agaaaatgga gaacttacgc ctgttgatgt aatgcacagc aacaaacaag
    12661 agttaaaaga tgctattaaa aatataagta attatgatat agaaacgata gtttttagtc
    12721 atggtggatt atatcaaaga gacataattg gaacattgaa aaacctaatt attgagtagt
    12781 actatatgat ttacaataat ttcttaaatt acttaaaatt atatctaaag atgtagaatc
    12841 aaaattaaat ataagtaata agaattttgc tttaaaatta tacttaag
//
LOCUS       DAMJWP010000092        12803 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.096, whole genome shotgun sequence.
ACCESSION   DAMJWP010000092 DAMJWP010000000
VERSION     DAMJWP010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12803
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.096"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            174..842
                     /locus_tag="PC700_002141"
     CDS             174..842
                     /locus_tag="PC700_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581449.1"
                     /translation="MSKLESTTEKVEVKWYGYVALILGALFFSGIFKDAPGPLKVLDF
                     NNVLGSFGTLGTINDGVGTLASNFRGDGGTGPRDGWLYALTLIPSVMFALGVVRVIDH
                     LDGMKAAQKLLSPLLKPLLGLPGFAGLTLIASLQSTDAAASMTKELKDDGYIDEKQKA
                     VFCAFQFSGASAITNFFASGAALFPFIGDVPIFIPLALILIMKFVGANLLRLYLNKFD
                     KGEA"
     gene            844..1329
                     /locus_tag="PC700_002142"
     CDS             844..1329
                     /locus_tag="PC700_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HDF2581450.1"
                     /translation="MSEVAKKEVKVKKGNVIDAFIGGARNGFQISTNSMAPNVIFGFA
                     IISVFNLTGLLDVIGTIFTPVMSIFGLPGVAATAIMTIFISMGGASGVIAGLFTAGQI
                     SQAHVAILLPMLLLSGAQIQFMGRLLGTAELKTKYYPHMFVIATLNGCIAMLIMNYFV
                     V"
     gene            1390..2511
                     /locus_tag="PC700_002143"
     CDS             1390..2511
                     /locus_tag="PC700_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HDF2581451.1"
                     /translation="MGTNKDYIEYVHKVYDYLHTIPEKGFEEYKTSEFVLKELEKMGF
                     ENINKGVAGTGIIVTIDSKKEGPVFAIRADMDALEFIIDGEKKMIHACGHDGHTSMLL
                     TAAKLIKERNLVKRGKLKILFQPGEEKLYGALRVIDSGLLDDVEEMVGMHLRPGQDCR
                     LGKAIPAMYHCASCIMQFTIKGITCHGSKPHLGVNAVDAVCLAVNAINAIRVNPAIPN
                     SIKVTNISVKGETYNNIPDEAFMALDIRAESNDIIEELKEKAIHAVKTAVSSINAEAV
                     LISKDGVPAAEFDEELVNLAEETIKDVLGADASIGIFKNSGGEDFHYYTQKLKCKTTY
                     IGLGADATPGFHNPNVTFDTKALEYGVDIWCRLVEKRLG"
     gene            2792..3982
                     /locus_tag="PC700_002144"
     CDS             2792..3982
                     /locus_tag="PC700_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2581452.1"
                     /translation="MSKYNFDRVIDRVGTDCVKWDFRTNCSTKAQKDGLPFWIADMDF
                     ECAEPIIEALRKKVEHKIFGYSSNDSDKYFNAVCDWYKRRFNWEINRKDIIFSPGVVP
                     AVAILVRILTNSNEGVIVQKPVYYPFEAKIKSNNRDIVNNPLIYENGTYRMDYDDLEE
                     KAKCSNNKVLILCSPHNPVGRVWREDELKKVVEICKKYDLWIIADEIHSDLVRKGFKH
                     TPLQSLCPEYKDKIITCIAPSKTFNLAGMQLSNIIVNNDELKKKYQEEVTAVGVATSP
                     NPFAIVATIAAYNESEDWLNELNDYLDNNIQFIDEYLKENLPKVKLVYPEGTYLAWLD
                     FTAYGLNEVELEDLMFKKANVLFDEGYIFGKEGIGFERINVACPQSLLKECMDRLKTI
                     FENL"
     misc_binding    4116..4371
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4430..5431
                     /locus_tag="PC700_002145"
     CDS             4430..5431
                     /locus_tag="PC700_002145"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HDF2581453.1"
                     /translation="MSTIIPKDYKSSLNVIDTQIAIKKLKDFFEMRLSNELNLIRVSS
                     PLFVLPETGANDNLNGEKAVSFDIPFMNKNAEIVQSLAKWKRLALKKYGFEVGSGLYT
                     DMNAIRKNEELDNIHSLYVDQWDWELVIDKSSRNEKTLKDVVKRLYGVFKDTEIFVCS
                     MYEGIKEILPEEITFITSQELEDMYPELSPKEREDKIVKEKKAVFITQIGKTLISGEK
                     HDGRSPDYDDWELNGDILFYNPVLDSALELSSMGIRVDEESLDRQLKLAGCEERKEFD
                     YHKMLLNGELPYTIGGGIGQSRICMYFLQKAHIGEVQVGVWPQDMIQNCSSAGIELL"
     gene            5535..5924
                     /locus_tag="PC700_002146"
     CDS             5535..5924
                     /locus_tag="PC700_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HDF2581454.1"
                     /translation="MEFLKIIINIVLDILKKILVRFKNAKFGLVFVFDLLKLPDFMTD
                     KRINIVDKIKVISVLIFTISYFVSGVDIIPEMIAGAFGFIDDAIVLIWSIGIVNEEIN
                     KYRVIIKKDKHSNIIENVEFSIKDEEE"
     gene            6085..7293
                     /locus_tag="PC700_002147"
     CDS             6085..7293
                     /locus_tag="PC700_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HDF2581455.1"
                     /translation="MKKKIGLVPKLIIGIIVGILIGSYAPEIIVQILVTVSTLFSAFL
                     KFVIPFIIIGFVTAGIADLATGAGKLLGITTGIAYGSTLIAGLLSFAVSTLIFPNFID
                     ASVASQIGDPEAGMLAPIFTIPLEPMVDVTAAIVFAFVMGLGISALRNHGKGETLFNF
                     FQEFQGIVTKTLSTVIIPLLPLYIAGTFANITIAGEVWTILGVFWKVYLVVIPLHFVY
                     MACQFTAAGVFSGKNPVRMLKNQVPGYLTAIGTQSSAATIPVNVGCAEKNGVSKQIRE
                     FVVPLCATIHLSGSMISITGFTTAVLMMNGMPHGIGVLFPYIAMLGIAMVAAPGAPGG
                     AIMSALPFLPMVGIPSDGGLASLMIALYLTQDSFGTACNVSGDNAIAVVVDKINDGMS
                     KKGNKEEQSA"
     gene            complement(7380..8456)
                     /locus_tag="PC700_002148"
     CDS             complement(7380..8456)
                     /locus_tag="PC700_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2581456.1"
                     /translation="MSTNHGANLYSLSSKYGFSKEEFMDFSSNINPFGTSSLAKQYIV
                     NNIDMVSMYPDPDYIDLKTSISNYCKCSIDNIVLGSGATELISSFIHTINPKQALLLS
                     PAYSEYEKELSKINCSIEKYFAKEEDNFHINLENLIKTINAKDYDLVVICNPSNPTGF
                     AFTKVEVREILKNTDSFLMIDETYVEFTDTDTYSCTQLVDDYSNLFVIRGTSKFFSTP
                     GIRLGYGLISNTNVKNEINKNLDLWNINIIASKMGEIMFSDLDFISNTISLMNTERDY
                     LLKELKNIKSLDIYNTKGNFILCKIKTKELTAKSLREQLLPQKIIIRDCCSFEGLDEY
                     FFRVCILKPNENKLLISSLKAIFK"
     gene            8685..9212
                     /gene="hpt"
                     /locus_tag="PC700_002149"
     CDS             8685..9212
                     /gene="hpt"
                     /locus_tag="PC700_002149"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDF2581457.1"
                     /translation="MYKVTGKMLTEEQIKEKVYELGKKIEEDFKGEDLLVVGILKGAS
                     VFVSDLIRCIDLDVNIDFMSVTSYGNSTESSGTVKILKDLDVDIEGKNVLIVEDIIDS
                     GLTLSNLVAALKTRNPKSLKLCTLLDKPQRRKANIPVDYVGFVIEDKFIVGYGIDYAE
                     KYRNLPYIGIVEDVE"
     gene            9301..10233
                     /locus_tag="PC700_002150"
     CDS             9301..10233
                     /locus_tag="PC700_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDF2581458.1"
                     /translation="MDIQLNILLFFQNLRNPILNFIFLVFTISTEAPLLILITTIIYW
                     CINKKCGQKILFAIIGNFVVNLGIKEFVKAPRPIGIKGLESLRVSTAGGYSFPSAHTQ
                     TATTFWVSIMTIFKKRYLHLIGAVMVLGVGLSRLYLAVHWPIDVIVGWMLGIFFTVIF
                     IKIFDYIDDNKNYILLVVLLIPFIIVAIFLNSPEYFEKFGIIVGFVFGYMVEDRFVKF
                     NTDNNNKKINFSNKNKGSKNIIFSRICRFLVGIVTIGMLYIGIKLFISMLIVTLNISD
                     STMSIIFMNFIKNTVVVFYGIAGAPALFKLLKLN"
     gene            10295..11521
                     /locus_tag="PC700_002151"
     CDS             10295..11521
                     /locus_tag="PC700_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF2581459.1"
                     /translation="MKKVVIIGGGPAGMIAASTACEKGYDVTLIEKNHKLGKKLAITG
                     KGRCNITNACEIEELIENVPTNGKFLYSAFYTFTNDDVISMFNNLGVKTKTERGKRVF
                     PESDKAFDIVNALERQLKSKKVNILLNSKVEKIISKNNKIEKVILNDKKEIKCDSVVV
                     ATGGLSYPLTGSTGDGYKFAISQGHTIIDTKPSLIGIEVQESFTKDLEKLSLRNVEIR
                     VFNSKQKKVYSDFGELEFTRFGLDGPIIKSASCRMKDTRKENYTILLDLKPALDEEKL
                     DKRVQKDFQKYTNKKFEKALDDLLPKKLIPIIINLSEINANTVVHQISREQRKNLVHL
                     LKNLKFTVKRYRPIEEAIITSGGVKVNEINSSTMESKLVEGLFFAGEVIDIDAYTGGF
                     NLQIAFSTGYLAGFNC"
     gene            11605..11826
                     /locus_tag="PC700_002152"
     CDS             11605..11826
                     /locus_tag="PC700_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HDF2581460.1"
                     /translation="MASNNNNNRTVVPGAKEALNQMKLEIANELGMSNYQQVDKGNLT
                     ARENGYVGGYMTKKLVEMAEQQMAGKSNR"
     gene            12015..12185
                     /locus_tag="PC700_002153"
     CDS             12015..12185
                     /locus_tag="PC700_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581461.1"
                     /translation="MSSVNFEDLKNKFINSDLDEKIRIYTTTEGLSVEQFKELLKYYP
                     IQHLSKLEKALG"
     gene            complement(12278..12739)
                     /locus_tag="PC700_002154"
     CDS             complement(12278..12739)
                     /locus_tag="PC700_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581462.1"
                     /translation="MQIEQLDLETRNKIYCYTKKILRKYQKGITSGKLTADKFADNIL
                     SQHFISSILNEKIVNETNFKISYRNYIETLINIQNENLSNLRKKSTKTAKCFNISQIT
                     QLKNLLSNTGYNLLIPYKYLTARDIEGIVTLINTGSIELGNERIYNYISKA"
BASE COUNT         4949 a         1343 c         2265 g         4246 t
ORIGIN      
        1 aaatattata tttttaaact tgaaaagtaa gtaattataa gctatttaat cgctggaata
       61 aatttacaat aaaaaaataa atacaaaggc tttatcatta aggtattggc ataagttttg
      121 cttattaaac aagtatacta ataaaattca tttaaacagg agggagaatt tatatgagta
      181 aattagaatc aacaacagaa aaagttgagg taaaatggta tgggtatgta gcattaatat
      241 tgggagcatt atttttttca ggaatattta aagatgcacc aggaccatta aaagtattgg
      301 attttaacaa tgtattaggt tcttttggaa cattgggaac aattaatgat ggtgtaggta
      361 cattagcttc aaactttaga ggtgatggag gtactggccc tcgagatgga tggctatatg
      421 cattgacatt aataccatca gttatgtttg cacttggagt agttagagtt atagaccact
      481 tagatggaat gaaggcagct caaaagcttc ttagtccact attaaagcca ttattagggt
      541 tacctggatt tgcaggacta actttgatag ctagtttaca gtctacagat gcagctgcat
      601 ctatgactaa agaacttaaa gatgatgggt atatagatga aaaacaaaaa gctgtatttt
      661 gtgcatttca attttcaggt gcgtctgcaa ttacgaattt ctttgcttct ggagcagcac
      721 tatttccatt tataggagat gtaccaatat ttataccact tgctttaata ttaattatga
      781 agtttgttgg ggcgaactta cttagattat atttaaataa atttgacaag ggggaagctt
      841 aacatgagtg aggtagcaaa aaaagaagta aaagttaaaa aaggaaatgt gatagatgcg
      901 tttattggag gagctagaaa tggattccaa ataagtacaa attcaatggc tccaaatgtc
      961 atcttcggtt ttgctataat ttctgtgttc aatttaacag gtcttttgga tgtaataggg
     1021 acaatattta cacctgtaat gagtatcttt ggacttccag gtgttgcagc aacagctata
     1081 atgacaatat ttatatcaat gggaggagca tctggagtaa tcgcaggttt atttacagca
     1141 ggtcaaataa gtcaagcaca tgttgcaatt ctattgccaa tgttattgct ttcaggagcg
     1201 cagatacaat ttatggggag acttttggga acagctgagt taaaaactaa atattatcca
     1261 catatgtttg ttatagcaac tttaaatggt tgtattgcaa tgcttataat gaattatttc
     1321 gtagtatagg taattataat aaaaatttaa tataaatgga aagtttaaat tttaaggagg
     1381 agctagatta tgggaactaa caaagattat attgagtatg tacataaagt gtatgattat
     1441 ctacacacta taccagaaaa aggatttgaa gaatataaga catcagagtt tgttttaaaa
     1501 gagcttgaaa aaatggggtt tgaaaatatt aataaaggag ttgcaggaac tggtattata
     1561 gtaactatag attctaaaaa agaaggacca gtatttgcta taagagcaga tatggatgca
     1621 ttggaattta tcatagatgg agaaaagaaa atgatacatg cttgtggtca tgatggtcat
     1681 acttctatgc ttttaacagc tgcaaagtta ataaaagaga gaaatttagt aaaaagaggt
     1741 aagttaaaaa tactttttca accaggtgaa gaaaaattat atggagcatt aagagtaatt
     1801 gattctggtt tgttggatga tgtagaagaa atggtaggga tgcatttaag acctggacaa
     1861 gattgtaggt tgggaaaggc gatacctgca atgtaccatt gtgctagctg tataatgcaa
     1921 tttacgatta aaggtatcac ttgtcatggt tctaaaccac atttaggagt aaatgcagta
     1981 gatgctgtct gtttagcagt gaatgcaata aatgcaataa gagtaaatcc agctatacca
     2041 aattctataa aagtaacaaa tatatcagtt aagggagaaa cttataataa tataccagat
     2101 gaagcattta tggctttaga tataagggca gaaagtaatg atataataga agaattaaaa
     2161 gaaaaagcaa tacatgctgt aaaaactgct gtaagttcta taaatgcaga agctgtacta
     2221 atttccaaag atggagtacc agcagcagaa tttgatgaag aacttgtaaa cttggctgaa
     2281 gaaactataa aagatgtatt gggagctgat gcatctatag gtatatttaa aaattctggt
     2341 ggagaagatt tccattatta tacacaaaaa ctaaaatgta aaactacata tattggatta
     2401 ggagctgatg ctacaccagg atttcataat ccaaatgtaa catttgatac gaaagcttta
     2461 gaatatggtg tagatatatg gtgtagattg gttgaaaaga gattgggata atatccaaac
     2521 taataattct acatttaaaa ttataattat agaagttata gaaaaagatt gcttatgtta
     2581 attagaaaat aattaatatg agcaatctta ttttgatata atgaaactga ttttggaata
     2641 ttgaaaaaat atgatggaaa agtaattata ttttaaatct attggacaaa aaagtgatat
     2701 tattataata ttaagaattg taaaataata ccaaagtgca tctagtaatg gacttaacaa
     2761 ttataaaaat tttattctta ggagggaaaa aatgagtaag tataattttg acagagtaat
     2821 agatagagta ggaacagatt gtgtaaaatg ggattttaga actaattgtt caacaaaagc
     2881 acaaaaagat ggtttgcctt tttggattgc tgatatggat tttgaatgtg cagaaccaat
     2941 aatagaagca ttacgtaaaa aagttgaaca taaaatattt ggatatagtt caaatgatag
     3001 tgataaatac tttaatgcag tttgtgattg gtataaaagg agatttaact gggaaataaa
     3061 tagaaaagat attattttta gtcctggtgt tgtacctgct gttgctattt tagtaagaat
     3121 attaactaat tctaatgaag gtgtaatagt ccaaaaacca gtgtattacc catttgaagc
     3181 taagataaag agcaataata gggacattgt aaacaatcct ctaatatatg agaatggtac
     3241 ttataggatg gattatgatg atttagaaga aaaagctaag tgtagcaaca ataaagtact
     3301 gatactttgt agtcctcaca accctgttgg aagagtttgg agagaagatg aattaaagaa
     3361 ggttgtagaa atatgtaaaa aatatgacct atggataata gctgatgaga tacattctga
     3421 tttagttaga aaaggattta aacatactcc tttacagtca ttatgcccag agtacaagga
     3481 taaaataatt acatgtatag ctccaagtaa aacatttaat ctggcaggaa tgcagttgtc
     3541 taatataata gtgaataatg atgaactaaa aaagaaatat caagaagagg ttacagctgt
     3601 tggagttgca acttcaccaa atccctttgc tattgtagct acaatagcag catataatga
     3661 aagtgaagac tggcttaatg aattaaatga ttacttagat aataatatac aatttattga
     3721 tgaatactta aaagaaaacc taccaaaagt aaaacttgtt tatccagaag gaacatatct
     3781 agcatggtta gattttactg cttatggatt aaatgaagta gaacttgagg atttaatgtt
     3841 taaaaaagct aatgtattat ttgatgaagg ttatatattt ggaaaagaag gaataggatt
     3901 tgaaagaata aatgtagctt gtccacagtc tttattaaaa gaatgtatgg acagattaaa
     3961 gactatattt gaaaatttat aaagtatagg taaaaattta taaagtgttt aaaaataaaa
     4021 aaataataaa cttgacaaat tttaactaat cacttatcat actattatga ataaatagaa
     4081 aacataagag agctaaattt tattgaatta aactcttaaa gactttgaaa ggaagagtag
     4141 ttatagaatg taattaaagc gagttggtga tggtgtgaga ccaatatgaa gtctataatg
     4201 aagaacatcc tggagttgct aaccgaaatt ttaaaagagt aggcttagtc ggaaccaaac
     4261 cgttatcata ttggggtgta tcgagtaata tctgtacatt gtgagctggg tttgaattta
     4321 tttcaaacta atcagggtgg taccgcggaa aagcaacctt tcgtcccttt tatataggga
     4381 tgaagggttt ttttaatgga aaaatatata aatataggga gtgaatgata tgagcacaat
     4441 aataccaaag gattataaaa gtagtttgaa cgtaattgat acacaaatag ctataaaaaa
     4501 attaaaagat ttttttgaaa tgagactaag taatgaatta aatcttataa gagtttcttc
     4561 tccgttattt gtattgccag aaacaggtgc aaatgataat ttaaatggag aaaaggctgt
     4621 aagctttgat ataccattta tgaataaaaa tgcagaaatt gttcaatcac ttgcaaaatg
     4681 gaaaagatta gcccttaaaa aatatgggtt tgaagttggt agtggtttat atacagatat
     4741 gaatgcaata agaaaaaatg aagaattaga caatatacat tctctttatg tagaccaatg
     4801 ggattgggaa ttggttatag ataaaagtag tagaaatgaa aagactctaa aagatgttgt
     4861 aaaaagattg tatggagtat ttaaagatac tgaaattttt gtatgtagta tgtatgaagg
     4921 tataaaagaa atattaccag aagaaataac ttttataaca tcacaagagt tagaagatat
     4981 gtatccagaa ttatcaccga aggaaagaga agataaaatt gtaaaagaga agaaagcagt
     5041 atttataact caaataggaa aaacattaat atcaggagaa aaacatgatg gaagaagccc
     5101 tgattatgat gattgggaat tgaatgggga tatattattt tacaatcctg ttttagattc
     5161 agcattagaa ttatcttcaa tgggaattag agttgatgaa gaatctcttg atagacaatt
     5221 gaaacttgct ggatgtgaag agagaaaaga gtttgactat cataagatgc ttttaaatgg
     5281 cgaacttccg tatacaattg gtggaggaat tggtcagtca agaatatgta tgtatttctt
     5341 acaaaaagct catataggag aagtacaagt tggagtatgg ccacaggata tgattcaaaa
     5401 ttgtagtagt gcaggaattg aattattata atttaaatat aaactaattg ataaaattta
     5461 tatctaatat gttcatcaaa ttaattatat cggttataat gtagaataac ctataattat
     5521 aagaggtgat aaatatggag tttttaaaaa taatcattaa tatagtttta gatattttaa
     5581 aaaaaatttt ggtcagattt aaaaatgcta aatttggttt ggtttttgta tttgatttac
     5641 ttaaactacc tgattttatg acagataaaa gaatcaatat agttgataaa ataaaggtaa
     5701 tttctgtatt aatttttact atatcctact ttgtatctgg ggttgatata attccagaga
     5761 tgattgcagg ggcatttggt tttatagatg atgctatagt tttaatttgg agtataggta
     5821 tagtgaatga agaaattaat aagtatagag ttattataaa aaaagataaa cattcaaata
     5881 taattgaaaa tgtagaattt tctataaaag atgaagaaga ataacaattt atatatgtta
     5941 ttcttttttt tattaacaaa aaatggttaa attgttgacg gagaggtaaa actctattat
     6001 cataataagt ggatatccaa tatttaacta agtattcaat gtgcaagaat gcttaattta
     6061 aaaaagttat ggagggtaaa tattatgaag aaaaaaatag gtttagtgcc caaactaatc
     6121 atcggtatca tagtcggtat tttaatcgga agttatgcac cagaaataat agtacaaata
     6181 ttagtaacag ttagtacctt attctcagca ttcctaaaat ttgttatacc atttataatt
     6241 ataggttttg taacagcagg tatagctgat ttagcaacag gtgcaggaaa gttattgggg
     6301 ataactacgg gaatagcata tggttctacc ttaatagcag gattattatc atttgcagta
     6361 tctacattaa tcttcccaaa ttttattgat gcaagtgttg caagtcaaat tggagaccca
     6421 gaggcaggaa tgttagctcc aatattcaca attccattag agcctatggt agatgtaaca
     6481 gctgcaatcg tatttgcatt tgtaatggga cttggaatat cagctttaag aaatcatgga
     6541 aaaggagaga ctttatttaa tttcttccaa gaatttcaag gaatcgtaac gaaaacgttg
     6601 tcaacagtga ttataccttt attaccttta tacatagcag gaacttttgc aaatataact
     6661 atagcagggg aagtatggac aatattaggg gtattctgga aagtatactt agtagtaata
     6721 cctttacatt ttgtatatat ggcatgtcaa ttcacagcag caggtgtatt ttcaggaaaa
     6781 aatccagtaa gaatgttaaa aaatcaagta cctggatatt taacagctat aggaactcaa
     6841 tcttcagcag caactatacc agttaatgtt ggttgtgcag aaaaaaatgg agtaagtaag
     6901 caaataagag aatttgttgt acctctttgt gcaacaatac atttatcagg tagtatgata
     6961 tcaataacag gatttacaac tgcagtttta atgatgaatg gtatgccaca tggaataggt
     7021 gtattattcc cttatattgc aatgttaggt atagcaatgg ttgcagcacc aggagcacca
     7081 ggtggggcta taatgagtgc acttcctttc ctaccaatgg ttggaatacc atcagatggt
     7141 gggcttgcaa gtttaatgat agctttatac ttaactcaag acagttttgg tacagcatgt
     7201 aatgtatcag gagataatgc aatagcagta gtagtagata aaataaatga tggaatgagt
     7261 aaaaaaggaa acaaagaaga gcaaagtgct tagtttttta ataatcaaaa atgagttatc
     7321 tcaaatagag atagctcatt ttttatggta taaaaaaatt ataaaataaa gggatttttt
     7381 tatttaaaaa ttgctttaag agatgatatt aataatttat tttcattagg ttttaatata
     7441 catactctaa agaaatactc atctagacct tcaaatgaac aacaatctct tattattatt
     7501 ttttgtggaa gcagttgttc tcgaagcgat ttagcagtta attcctttgt tttgatttta
     7561 cacaatatga aattaccttt agtattgtat atatctaagc tttttatatt cttaagctct
     7621 ttaagcaggt aatctctttc agtgttcatt aaggaaatag tatttgatat aaaatctaaa
     7681 tcactaaaca tgatttcccc cattttagaa gctattatgt ttatattcca taaatctaga
     7741 tttttattta tttcattttt gacattagtg ttagatataa gaccatatcc aagccttatt
     7801 ccaggcgttg aaaaaaattt tgatgtacct cttataacga ataaattaga ataatcatca
     7861 actaattgag tacaagagta tgtatctgta tcagtaaact ctacataagt ctcatcaatc
     7921 attaaaaatg agtctgtatt ttttaaaatt tctctcactt ctactttagt aaatgcaaat
     7981 ccagttggat tactaggatt acagattact accaaatcat aatctttagc attgatagtt
     8041 tttataagat tttctaaatt tatatgaaaa ttatcttctt cttttgcaaa gtatttttct
     8101 atagaacaat taattttaga taactctttt tcatactctg aataggctgg agagagtaaa
     8161 agagcctgtt taggattaat tgtatgtata aatgatgaaa ttagttctgt agctccactg
     8221 ccaagtacta tattatctat agaacatttg cagtaattgg atatagaagt ttttaagtct
     8281 atgtaatcag ggtcaggata catagatacc atatctatat tgtttacaat gtattgtttg
     8341 gctaaacttg atgtaccaaa aggatttata tttgagctaa aatccataaa ttcttccttt
     8401 gaaaagccat acttactaga taaactatat aaattagctc catgatttgt gctcataaaa
     8461 gtacctccaa aaataaaatt atataatcga taaaaataac tgattaatat atgtacatat
     8521 agattataat acaaaggaat cgttattcca aatttttaat ggaaaaagca aaaaagtatg
     8581 gataagctgt taatttaggt ctaaaaatga tgaaaaagat tttaattgat attttggtat
     8641 gctataatat ataagttaat agaaggaaag cgggaggata caaaatgtac aaagttacag
     8701 gaaaaatgtt gacagaagaa caaataaaag aaaaagttta tgaattagga aaaaagatag
     8761 aagaagactt taaaggcgaa gatttactag tggtaggtat acttaaagga gctagtgtat
     8821 ttgtttcaga tttaataaga tgtatagatt tggatgtaaa tatagatttt atgagtgtta
     8881 caagttatgg aaactcaaca gagtcttctg gaacagttaa aatattaaaa gatttagatg
     8941 tagatattga aggaaagaat gtgttgattg tagaggatat aatagattct ggtttaacat
     9001 taagtaatct agttgcagct ttaaaaacta gaaatccaaa atctttaaaa ctatgtactc
     9061 tacttgacaa acctcagaga agaaaagcta atatacctgt agactatgtt ggatttgtta
     9121 tagaagacaa atttattgtt ggttatggaa tagactatgc tgaaaagtat agaaatttac
     9181 cttatatagg tatagttgag gatgtagaat agatttaaat gtgaatcaaa agcactaatt
     9241 tctaagggag ttagtgcttt ttagttaatt atataaaatt ttagtaaagg agacatctat
     9301 atggatattc agttaaatat attattattt ttccaaaatt taagaaatcc aattttgaac
     9361 tttatatttc ttgtctttac aataagcaca gaagcaccgt tattaatatt aattacaaca
     9421 attatatact ggtgtataaa taaaaaatgt ggacagaaaa tactgtttgc tataattgga
     9481 aattttgttg taaatttagg catcaaagag tttgtaaaag ctcccagacc aataggaata
     9541 aaaggtcttg aatcattgag agtttctact gctggtggtt attcttttcc aagtgctcat
     9601 actcaaactg caacaacatt ttgggttagt attatgacta tatttaaaaa gagatatctt
     9661 catttaatag gtgcagttat ggttttagga gtaggattgt ctaggctgta tttagctgtt
     9721 cattggccaa tagatgtgat agtgggttgg atgttaggaa tattttttac agttatattt
     9781 ataaaaatat ttgactatat tgatgataat aaaaattata ttttacttgt agttttactt
     9841 ataccattta taatagtagc tatatttcta aatagccctg aatattttga aaaatttggt
     9901 ataatagtag gatttgtatt tggatatatg gttgaggata gatttgttaa atttaataca
     9961 gataataata acaaaaaaat aaattttagc aataaaaata aggggagtaa aaatataatt
    10021 ttttcaagaa tatgtaggtt tttggttggg atagtaacaa taggaatgtt atatattggc
    10081 attaagttgt ttatttcaat gttaatagtc actttaaata taagtgactc tactatgagt
    10141 ataattttta tgaattttat taagaataca gtagttgttt tttatggaat agcaggagct
    10201 ccagcattat ttaaactttt aaaattaaat tgattttaag gtatagagtc aatagtttaa
    10261 attaaaaaaa ttaagggtag aaaagagtga ttatttgaag aaggtagtaa taataggtgg
    10321 tggaccagca gggatgatag ccgcatcaac tgcatgtgag aaaggctatg atgtaacatt
    10381 aattgagaaa aatcataaat taggaaaaaa acttgcaata acaggaaaag gtagatgtaa
    10441 tattactaac gcttgtgaaa tagaggaatt aatagaaaat gtacctacaa atggaaaatt
    10501 tctttatagt gctttttata cttttacaaa tgatgatgta ataagtatgt ttaataatct
    10561 tggagtaaag actaaaacag aaagaggaaa aagggttttt ccagagagtg ataaagcctt
    10621 tgatattgta aatgctcttg aaagacaact taaaagtaag aaagtaaata tattattaaa
    10681 ttcaaaagta gaaaaaataa tatctaaaaa taataaaatt gagaaagtaa ttttaaacga
    10741 taaaaaagaa attaagtgtg attcagttgt agtagcaact ggtggattaa gttatccact
    10801 tactggttct actggtgatg gatataaatt tgcaatttca caggggcaca caattataga
    10861 tacaaaacca tctttaattg gtatagaagt acaagaaagt tttacaaaag atttagaaaa
    10921 attatcattg agaaatgttg aaataagggt gtttaatagc aaacaaaaga aagtttatag
    10981 tgattttgga gaacttgaat ttactagatt tggtttagat ggacctataa tcaagtctgc
    11041 aagttgtaga atgaaagata caaggaaaga aaattacact atattattag atttaaaacc
    11101 tgcattagat gaggaaaaac tggataaaag agttcaaaaa gattttcaga agtatacaaa
    11161 taaaaaattt gaaaaagcat tagatgattt acttccaaaa aaactaatac ctataataat
    11221 aaacttaagt gaaattaatg caaatacggt tgttcaccaa atttctagag agcagagaaa
    11281 aaacttagtc catttattaa aaaatttaaa gtttacagta aagagataca gacctattga
    11341 agaagctata ataacttctg gtggtgtaaa agtaaacgaa ataaattcaa gcacaatgga
    11401 atcaaaatta gtagaaggtt tattttttgc tggagaagta atagatatag atgcatatac
    11461 aggtggattc aatttgcaaa tagctttttc tactggatat ttggcaggtt ttaattgcta
    11521 aaaattacct gaattaaaaa tgtttaaaat cacataatat tatcagcaac acaaaataaa
    11581 ttaaacagaa ggaaggtaat caacatggca agtaacaata acaacaacag aacagtagta
    11641 ccaggagcaa aagaggcttt aaaccaaatg aaattagaaa tagctaatga acttggtatg
    11701 agtaattatc aacaagttga taaaggaaac cttactgcaa gagaaaatgg ttatgtaggt
    11761 ggatacatga ctaaaaaatt agttgaaatg gctgaacaac aaatggctgg aaaaagcaac
    11821 agatagttat aaataattat ttataactat tggctattag tttagctaga gctatctcaa
    11881 atatagaaag taatttctaa attgagatag ctttttttta ttaaattatt aaatagatat
    11941 atttttatgt aataaatttt atgtatttgg ataaaataat acaaatgaat atgttacata
    12001 aggagtgatg taaaatgagt agtgtaaatt ttgaggattt aaaaaacaaa tttattaata
    12061 gtgacttaga tgaaaaaata agaatatata caacaacaga aggtttatca gtagaacaat
    12121 ttaaagagct actaaaatat tatccaatac aacatttatc taaactagaa aaagcacttg
    12181 gatagcagta tttagtccaa aattaaaaag gcaatttatt taatattaaa aatatttttt
    12241 attaaataaa ttgccttttt ataataggtt taaattatta tgcttttgat atataattat
    12301 agattctctc atttccaagt tcaatagacc cagtattaat aagagtaacg attccttcta
    12361 tatctctagc agttaagtat ttatatggaa ttaataaatt ataaccagta ttacttagta
    12421 agttttttaa ttgggttatt tgagagatat tgaaacattt tgcagtttta gtagattttt
    12481 ttcttaagtt agataaattt tcattttgaa tatttattaa agtttctata tagtttctat
    12541 aagatatctt aaaatttgtc tcattgacta ttttttcatt aagtatacta ctgataaaat
    12601 gttgagacaa tatattatct gcaaatttat ctgctgttaa ttttccagat gttatgcctt
    12661 tttgatattt tctaagaatt tttttagtat agcagtatat tttatttctc gtttctaaat
    12721 cgagttgttc tatttgcaca aaaccacttc cttctaatta taaaaatatt tattaaaaat
    12781 ctatacttaa ctaataaagc ttc
//
LOCUS       DAMJWP010000093        12780 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.097, whole genome shotgun sequence.
ACCESSION   DAMJWP010000093 DAMJWP010000000
VERSION     DAMJWP010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12780
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.097"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            123..860
                     /gene="cas6"
                     /locus_tag="PC700_002155"
     CDS             123..860
                     /gene="cas6"
                     /locus_tag="PC700_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HDF2581463.1"
                     /translation="MSRISIFMESCNGKFPNENSMLSVSFIKNILNAENKDFAKNIFN
                     YGEERKNNKQIKDINTAIYIPNLIRSKNELLVDGDIIFNISFYNYSMFSKLYNGILKV
                     KELEYKGYRFKIKNIKIHKEHEIKENGVIFKTMSPIIVKNKEGKYLDVEDSNYIECLN
                     YIANLTLESIRGSGLRKPLEFIPLNFKKRVLKEKIRGFKVREYYYINAYAGTFFLKGN
                     MEDLNALYKMGIGYRRTENAGMVDILK"
     gene            865..2340
                     /locus_tag="PC700_002156"
     CDS             865..2340
                     /locus_tag="PC700_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581464.1"
                     /translation="MREIKAKFYRSGDIVRDIGIVYFYELLMDLKNELEKNDYKLKFT
                     CELNRNYLSLESAEYIDPKYISDYILENQLFKVFKNGKKETLEKIFSNIDNLTYVNFI
                     EELDKSSATEKQKEGIKKDFKNRRFPYVRNSGKYGLNTSLENMETNVKRIVDTVIKSN
                     IEIDEIDKLDLTQYEEKDSVCNVCNINKTTKLDIDLRERKDSKYNFLFRGAEKSGFKR
                     SGQVESNICFECEFFNLMCLLYINLKRPMVFAYTNDLRELAFLNHKIMLKRKMYSDKS
                     FYKKLLHEKISSIRLYRFDIDTNKGIILKFDSIIEYKELLKKIELIDIIDNYNFSREP
                     GNTRNLGKKMIDNGNLSNLKELLLDNISVLRQLTGTSREMDFVSSSYNIGLYIKLCWI
                     VDGGEEYKMKNYMESNLIYSRVGKDLFNKMTDESRRKNFSMKVIQLLKSNDRTQLFQT
                     IMHVLVSNGILIGEGFVEGLMQSSELELNTNVGLFIQEIMK"
     gene            2360..3373
                     /locus_tag="PC700_002157"
     CDS             2360..3373
                     /locus_tag="PC700_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HDF2581465.1"
                     /translation="MNEKRVKGVTLTILTESPVPLSYDQGYGNYTPIKKEQYREKIHA
                     KTSIATITYDLRRMLHQEYGWNLSNIIFGKKGNIYPSIKQNVGCVDENGLETDVFGYL
                     IPLKDKGSISKASPLRIIPFRSLNSYRGSTQLITNRGFLSSEFGRKYYDEKEENEVLR
                     DENFPTSQALAIEETLSDYYVYTITLELDRIGVVEVEDGKLLLPEERKFMSKELREKA
                     VKDILDAITVFTRNIKHSSVLLKPLAVMGGAFDKVVPFFWDDVDYNADSGEINLEGVI
                     ETIESYSLKESNTILAINDKLKISNKKELNKFNLSKYPVKEIKNLADRLEIGEDNMWY
                     LKE"
     gene            3389..4195
                     /gene="cas5"
                     /locus_tag="PC700_002158"
     CDS             3389..4195
                     /gene="cas5"
                     /locus_tag="PC700_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HDF2581466.1"
                     /translation="MKVLQCKLKQPTAHYRDPKVFQNEYISTLNLPSKTTIMGMITYL
                     CDRRLNSDIDIGIIGTHHHRELEFSRGENIDFWNEYTNMRKGKDKEKFLLQGNYYDYY
                     KEHKAQNSILNYEVLKEVELTIFISCKDDEELEFIKKKLESPCKYANLGRKEDFVIPS
                     EKGCFVKEVELKEVMPMNTRDAIKENIKLKNTYVRVDLRDKENVETIINQGVLIALPY
                     KYKDLEANRDDRVYEFCHYIYVDNDGIYPKNIKVNVCNVCIDTKEVFTWL"
     gene            4222..6630
                     /locus_tag="PC700_002159"
     CDS             4222..6630
                     /locus_tag="PC700_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HDF2581467.1"
                     /translation="MKNIMQKEILAKSYNLRTNQQEQTLVEHIKDLFEVLESILELNL
                     YSDKDVEILKICCALHDLGKINSIMQQKMEINNKISYSCSEEERKKLESDKKSLKKIA
                     RHNIFSGAFLKDILEKMNLLEEDKLYIYKSIMLHHGNYEDYMRLSTSKVQEEIYDYIE
                     KGILEKEEFNLKDIESYINDILNVDFKFGEDVLDYDYIDKLSESMFIDSDYNNGQIDD
                     SVLNYRKFKYILYKGMLNLIDHSASSRQKGIKFYNDFTDEEIDDMILNEIYKSQGNLE
                     KNNIEFNTIQKRLRELSGRNVLTVAFTGSGKTAADYRKTFKRKFFLVPNKISAESFYR
                     KNIFQNKNDLDTRSSNDYIGLLHGDINLYSENEDNGEHDFVLTLRDIDLSINFCKPCV
                     IATVDQLLLSMFKFPGYEKIFAAVKNASITVDEVHLLEPKMFLILIYFMQFACKYLDV
                     DFHLMTATLPKSYKEQMINKGIIFQEESNENVTDKGEIVFIESNKEEEICEGKDVKVS
                     FIKEKEIKSIVEGALENKQKILIIKNTIDSVNRTYEFLKENLSDKYGGVDIDVLHSRF
                     KFKDKKEKYSKILNGEGDIWISTQSVEISLDLDFNIIISDLATMDSLIQRMGRCNRNN
                     KYEYGNFYILPSEDKIYDNKLKNTTKNILKDILKTNSIFTMGIRKTILDDYYDNSVVK
                     NYFEENFISCDKEIKNIYGINKEIFDGLDLIFNFEPYKNIVDSKKEAAKIFRDVDVSY
                     KIILEEDFYKEDRELQQDSIQVSGFIFNRLYYYGLISKVEGYMVLKSSSKFEYNSTVG
                     LILK"
     gene            7071..8018
                     /gene="prmA"
                     /locus_tag="PC700_002160"
     CDS             7071..8018
                     /gene="prmA"
                     /locus_tag="PC700_002160"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDF2581468.1"
                     /translation="MNNWIEVTIKTTTEAVEPITNILYEQGAGGAVIEDPKDFLFQKK
                     NELDWDYVEEEVFKKNEEDDVLIKTYVSEEKNVMEFVEIIKQKVLGLKDFGIDIGEGS
                     VSLDQVNEADWANAWKAYYKPTKVGQRVVVKPTWEDYAMQDGDLIIELDPGMAFGTGT
                     HETTSMCIRELEKYVNKDSKVFDIGCGSGILAIAAAKLGAKEVVAVDLDEVAVKVAKE
                     NVLENKVEKSVSVMHGNLTDVIKDKADVIVANIIADIIKILAKDVQNFMKEDAIFISS
                     GIILDKVEEVKESLIENGFEIVEVQKLGEWSAIVSKLKK"
     gene            8037..8795
                     /locus_tag="PC700_002161"
     CDS             8037..8795
                     /locus_tag="PC700_002161"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDF2581469.1"
                     /translation="MDRFFVEKNNINLQDKTCTIEGEDVKHISKVLRCKLGEKLEICD
                     KNNNEYICEIMNIDKSIVNLEILEKVDINRESELKVRLYQGLPKAPKMEMILQKLTEV
                     GVEEIILVQTKRSVVKVDDKKEDKKFERWERIIYEAAKQSKRGKIPKLRGVLSFKEAL
                     EDMKKNNVNICPYENERTVSIKHALKKCDSNIDSVGIFIGPEGGFSEEEIEQIQKNNC
                     NVVSLGPRILRTETASVVASTIALYELSDLGGEK"
     gene            8797..10095
                     /gene="mtaB"
                     /locus_tag="PC700_002162"
     CDS             8797..10095
                     /gene="mtaB"
                     /locus_tag="PC700_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HDF2581470.1"
                     /translation="MKKVAFYTLGCKVNQYETEAMLELFEKDGYEQVNSEEYADVYVI
                     NTCTVTHMSDRKSRQYIRRVKKKNPDAIIAVVGCYSQVSPEEILDIEEVNLVMGTNDR
                     RKIVEEIKKINSSKKVSTVDDIMKVKAFEEIEISQTNGKTRAFMKIQDGCDRFCTYCI
                     IPYARGRVRSRDIDSIVDEVKKLANNGYKEVVLTGIHVASYGKDLKDRDIKLLDVIKQ
                     INQIEKIERIRLSSVEPILFTDEFVNEVLKMDKVCPHYHLSLQSGCDETLKRMNRRYT
                     TLEYKTIVDRLRSKMPDVAITTDVIVGFPGETNEEFKKTYEFLKEIELSQMHIFKYSP
                     RKGTPAATMENQVDPQMKHFRSEQLLNLSKVNFNKFATKFIGRELDVLFEQNIEGNKY
                     EGLTSNYIRVVVESDKNIQGQILKVKINDVKDEYVEGILL"
     gene            10138..10488
                     /locus_tag="PC700_002163"
     CDS             10138..10488
                     /locus_tag="PC700_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HDF2581471.1"
                     /translation="MDCIFCKIANGEIPSTKVYEDDRVLAFNDLNPVAPYHILVVPKK
                     HYDSLIDIPDKEMDIVSHIHVVINKIAKEKGFDQTGFRVINNCGSDGGQEVKHLHYHI
                     LAGKKLPNYEAGQN"
     gene            10651..10830
                     /locus_tag="PC700_002164"
     CDS             10651..10830
                     /locus_tag="PC700_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDF2581472.1"
                     /translation="MSEVRVRENETLDSALRRFKRQCAMSGIMSEVRKREHYDKPSVK
                     RKKKAEAARRKNAKK"
     gene            10860..11303
                     /locus_tag="PC700_002165"
     CDS             10860..11303
                     /locus_tag="PC700_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HDF2581473.1"
                     /translation="MSLKQKLQEDLKSSMKNKDTVRKSVVTLIRASIKQYEVDNRVEL
                     DEDGIIDVIAKQLKQRRDALVEFEKAGREDLIKETEGEIEVLKEYLPQQLSEEELEEI
                     VKSTISEVGATSMKDMGKIMSVIQPKVKGRADGKLINKLVKQNLQ"
     gene            11414..11911
                     /locus_tag="PC700_002166"
     CDS             11414..11911
                     /locus_tag="PC700_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HDF2581474.1"
                     /translation="MIISVKEIRNFLINIFIPLIIGYLSSILTMIISGTDISTYYLQL
                     TKPSFAPPAFIFPIVWTILYILMGISSYLILRKGYNLPKVRDAIFYYGLQLVLNFIWS
                     ILFFGFGLRFTALIDIIIMILVSIIMISKFSKIDKRSGYINLIYLMWLVYAGFLNYFI
                     WFINK"
     gene            12104..12667
                     /gene="raiA"
                     /locus_tag="PC700_002167"
     CDS             12104..12667
                     /gene="raiA"
                     /locus_tag="PC700_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDF2581475.1"
                     /translation="MQIIVSGRQMKLTDGIKGYVDGKLSRLEKYLDPESEVKVTVSAK
                     KDRQKVEVTIIPINGQIIRAEDVEDDLYAAIDIVCDKLSRQVVKYKTKVKDKVQNNKS
                     IRFENLDFIDNSSEFDDYDYDDEEDENIVIERRKKFNVKPMSSEEAILQMELVGHNFY
                     MFRNQDNFEINIVYKRKAGGYGLIEQD"
BASE COUNT         5404 a         1124 c         2337 g         3915 t
ORIGIN      
        1 atatttaaaa aattaaaaaa caaaattaaa tttattatca aaaaatcatt ttatgatatg
       61 ttttattttt tatattacaa atagagaata atgaattata ctagaaagta ggaggtgaaa
      121 atatgagcag aatttcaatt tttatggagt catgtaatgg caaatttcca aatgaaaatt
      181 caatgttgtc agtttccttt ataaaaaata ttttaaatgc agaaaacaaa gattttgcta
      241 aaaatatatt taactatggg gaagaaagaa aaaacaataa gcaaataaag gatataaata
      301 cagccatcta cataccaaat cttataagaa gcaaaaatga gttgcttgtt gatggagata
      361 taatattcaa tataagtttt tataattatt ctatgttcag taaattatac aatggaattt
      421 taaaagtaaa ggagctagaa tataaaggtt atagatttaa aataaaaaat ataaaaatac
      481 ataaagaaca cgagataaaa gaaaatggtg ttatatttaa aactatgtct cctataatag
      541 taaagaataa agaaggtaaa tatttagatg tagaagattc aaattatata gaatgtttaa
      601 actatatagc taatctaact ctggagagta taagaggtag tgggcttaga aagccattgg
      661 aatttatacc tcttaacttc aaaaaaagag tgttaaaaga aaagattcgt ggatttaaag
      721 tgagagaata ttactatata aatgcttatg caggaacatt ttttttaaaa ggaaatatgg
      781 aagatttaaa tgcactgtat aaaatgggaa ttggatatag aaggacggaa aatgcaggaa
      841 tggttgatat attaaagtag cgaggtgaga gaaataaaag ctaaatttta tagatcaggt
      901 gatattgtaa gagatattgg cattgtttac ttttatgaat tactaatgga tttaaaaaac
      961 gaactagaaa aaaatgatta taagctaaaa tttacttgtg agttaaatag aaattattta
     1021 tctttagaat ctgcagaata tatagaccct aagtatatat ctgattatat attagaaaat
     1081 caacttttta aggtatttaa aaatgggaaa aaggaaacct tagaaaaaat atttagtaat
     1141 atagataatt taacgtatgt aaattttatt gaagaattag ataaatcttc tgcaactgag
     1201 aaacaaaagg aagggattaa aaaagatttt aaaaacagaa gatttccata tgttagaaat
     1261 agtggtaagt atggattaaa cacgagttta gaaaatatgg aaacaaatgt aaaaagaata
     1321 gtagatactg taattaaatc aaatatagag atagatgaaa tagataaact tgatttgaca
     1381 caatatgaag aaaaagatag tgtatgtaat gtttgtaata taaataaaac aacaaaacta
     1441 gatattgatt taagagagcg aaaggactct aagtataact ttttatttag aggtgcagaa
     1501 aaatcaggct ttaaaagaag tggacaagtt gaaagtaata tttgttttga gtgtgaattt
     1561 ttcaacttaa tgtgtctttt atatataaat ttaaaaagac caatggtttt tgcatatacg
     1621 aatgatttaa gggaattagc atttttaaat cataagataa tgcttaaaag aaagatgtat
     1681 tcagataaat cattttataa aaaacttttg catgaaaaaa taagttcgat tagactatat
     1741 aggtttgata ttgatacgaa taaaggtata attctaaagt ttgactctat tatagagtat
     1801 aaagaattat taaaaaaaat tgaacttata gatattatag ataattataa tttttcaaga
     1861 gagccaggga atactagaaa tttgggtaaa aaaatgattg ataatgggaa tttatcaaat
     1921 ttaaaagaat tattacttga taatataagt gttttaaggc aactaactgg aacttctcga
     1981 gaaatggatt ttgtatctag ctcatataat atcggtttat atataaaatt gtgttggatt
     2041 gtagatggag gggaggagta taaaatgaaa aactatatgg aaagtaattt aatatattca
     2101 agagtaggta aagacttatt taataaaatg acagatgaat ccagaagaaa aaatttttct
     2161 atgaaagtga ttcaactact taagtctaat gatagaactc agctatttca aactataatg
     2221 catgtgttag tttctaatgg aatcttaata ggagaagggt ttgtagaagg tctaatgcag
     2281 agttctgaat tagaattaaa tacgaatgta ggtctattta ttcaagaaat catgaaataa
     2341 aaaaggggga aaatttaaaa tgaatgaaaa aagagtaaaa ggagtaacat taactatact
     2401 gacagaaagc ccagttccac tttcatatga ccaagggtat ggaaattata caccaataaa
     2461 aaaagaacaa tatagagaaa aaatacatgc aaaaactagt atagccacaa taacatatga
     2521 tttaagaaga atgctccacc aagaatatgg ttggaattta agtaatatta tttttgggaa
     2581 aaaaggaaat atatatccat ctattaagca aaatgtagga tgtgtagatg aaaatggatt
     2641 agagactgat gtatttggtt atttgatacc acttaaagat aaaggttcta taagtaaggc
     2701 ttctccgctt agaataatac cttttaggtc attaaattca tatagaggtt caacacagtt
     2761 gataacaaat agaggttttt taagtagcga atttggtaga aaatattatg atgagaagga
     2821 agaaaatgaa gtacttagag atgaaaactt tccaacatca caagcattag caatagaaga
     2881 gactttatca gattactacg tttatacaat aacattggag cttgatagaa taggagtagt
     2941 agaagtagaa gatggaaagt tgcttttacc agaagaaaga aaatttatga gtaaggagtt
     3001 aagagaaaaa gcagtaaaag atatattaga tgcaattact gtgtttacaa gaaacataaa
     3061 acattcatca gtactgttaa aaccacttgc agtaatgggt ggagcttttg ataaagttgt
     3121 accgtttttt tgggatgatg tagattataa tgctgatagt ggtgagataa atttagaggg
     3181 agtcatagaa actatagaaa gttatagcct taaagaaagt aacacaatac ttgctatcaa
     3241 tgacaaatta aaaatttcta ataaaaagga attaaataag tttaatttaa gtaaataccc
     3301 tgtaaaagag attaaaaatc tagcagatag attggaaatt ggagaagata atatgtggta
     3361 tttaaaagag taggaggttt tttgaattat gaaagtatta caatgtaaac ttaagcaacc
     3421 aactgctcac tatagagacc caaaagtatt tcaaaatgaa tatataagta ctttaaattt
     3481 accatctaag accacaataa tgggaatgat aacgtattta tgtgatagaa gattaaactc
     3541 tgatatagat ataggaataa ttggaacaca tcatcataga gaactagaat tttcaagagg
     3601 agagaacatt gatttttgga atgaatatac aaatatgaga aaaggcaaag ataaagaaaa
     3661 gtttttgctt caaggaaact actatgatta ctataaagaa cataaagctc aaaatagcat
     3721 attaaactat gaagttctta aagaagtaga actgactata tttatttctt gcaaggatga
     3781 tgaagaactt gaatttatta aaaaaaagct tgaaagtcca tgtaaatatg caaatttagg
     3841 aagaaaagaa gattttgtaa taccaagtga aaaagggtgt tttgtgaaag aagttgagtt
     3901 aaaagaggtt atgcctatga atactcgtga tgctataaaa gaaaatataa aattaaagaa
     3961 tacatatgta agagttgact taagagataa agagaatgtc gagactataa taaatcaagg
     4021 tgtattgatt gcattacctt ataaatataa agatttagaa gcaaatagag atgatagagt
     4081 ttatgagttt tgccattata tatatgttga taatgatggt atatatccta aaaatattaa
     4141 ggtaaatgta tgtaatgtat gtattgatac aaaagaagtt tttacatggc tttaaattat
     4201 tttgaaagat aggtgtttat catgaaaaat atcatgcaaa aagaaatatt agctaagtca
     4261 tataatttaa gaactaatca acaagaacaa acattagttg aacatattaa ggatttattt
     4321 gaagtattag aatctatatt agaactaaat ctatattcag ataaagatgt agagatacta
     4381 aaaatatgtt gtgctttgca cgatttagga aaaattaatt caattatgca acaaaaaatg
     4441 gagattaata ataaaatatc gtatagttgc tcagaagaag agaggaaaaa gttagagtct
     4501 gacaaaaaat ctttaaaaaa gatagcaaga cacaatatat tttctggagc atttttaaaa
     4561 gatatattag aaaaaatgaa cttgttagaa gaagataagt tatatatata taaaagtata
     4621 atgttacatc atggaaacta tgaagattat atgaggttaa gtactagtaa agttcaagaa
     4681 gaaatttatg attatattga aaaaggcatt ttagaaaaag aagagtttaa tctgaaagat
     4741 atagaaagtt atataaatga cattttaaat gtagatttta aatttggtga agatgtattg
     4801 gattatgatt acatagataa attaagtgaa agtatgttta tagacagtga ttataacaat
     4861 ggtcaaatag atgattcagt tttaaattat agaaagttta agtatatttt atataaaggg
     4921 atgctgaact taatagacca tagtgcatca agtagacaga aaggtataaa attttataac
     4981 gattttactg atgaagaaat tgatgatatg attttaaatg agatatataa gagtcagggt
     5041 aatttagaaa aaaataatat agagttcaat actatacaaa aaaggttgag agaactttct
     5101 ggaagaaatg ttctgactgt tgcatttaca ggttcaggaa aaacagctgc agattataga
     5161 aagactttta aaagaaaatt ctttttagtg ccaaataaaa tatcagcaga aagtttttat
     5221 agaaaaaata tttttcaaaa taaaaatgat ttagatacaa gaagtagtaa tgattacatt
     5281 ggattacttc atggagatat aaacttatat tcagagaatg aagataatgg agaacatgat
     5341 tttgtattga cattaaggga tatagatttg agtataaatt tttgtaaacc ttgtgtaatt
     5401 gcaacagttg accagttatt acttagtatg tttaaatttc cgggatatga aaaaatattc
     5461 gcagccgtaa aaaatgcatc tataactgtt gatgaagtac atttattgga accaaagatg
     5521 tttttgattt tgatatattt tatgcagttt gcatgcaagt acttagatgt tgattttcac
     5581 ttaatgacag ctacacttcc aaaatcatat aaagagcaaa tgataaacaa gggaattatt
     5641 tttcaagaag aaagtaatga aaatgttact gataaaggag aaatagtttt tattgagagc
     5701 aacaaggaag aagaaatttg tgaaggtaaa gatgtaaagg tttcttttat aaaggaaaaa
     5761 gaaatcaagt caatagttga aggtgcttta gaaaataaac aaaaaattct tataataaag
     5821 aatactattg attcagtcaa tagaacatat gagtttttaa aagaaaattt atctgataag
     5881 tatggtggtg tagatataga tgttttacat agtagattta agttcaaaga taaaaaagag
     5941 aaatatagta aaatattaaa tggagagggt gatatttgga tatcaactca atcagtagaa
     6001 atatccttgg atttggattt caatataata ataagtgatt tagcaactat ggatagctta
     6061 atacaaagaa tgggtagatg caatagaaat aataaatatg aatatggaaa cttttatatt
     6121 ctacctagtg aagataaaat ttatgataac aaacttaaga acacaactaa aaacatctta
     6181 aaggacatat taaaaactaa ctctattttt acaatgggga ttagaaaaac tattttggat
     6241 gattactatg ataatagtgt agttaaaaac tattttgaag agaattttat aagttgtgat
     6301 aaagaaatta agaatattta tggtataaat aaagaaattt ttgatggatt ggatttaatt
     6361 tttaattttg aaccttacaa aaatattgtt gatagtaaga aggaagcagc taaaattttt
     6421 agagatgttg atgttagtta caaaattatt ctggaagaag atttttataa ggaagatagg
     6481 gagttacaac aagattcgat tcaagtatca gggtttatat ttaatagatt atattattat
     6541 ggattaataa gcaaagtaga aggatacatg gtactaaaat ctagtagtaa gtttgagtat
     6601 aatagtactg taggcttaat tttgaagtga gcatatgttt aaatataata tacctttaga
     6661 cattgaaaat acaaaaagta ctactgtaag cccactgtaa aaactatttt ttaaaaggaa
     6721 aaataagttg tgagatagag tgtctacaag tgtatataga taataaattt gaggtttaag
     6781 attaactaag tgggtttcag gtacatcgcg aaaataatta taagttggag attttgtatg
     6841 tttaagatta actaagtgga tttcaaatat tgatatagta gatataatag aagttttatt
     6901 attaggttta aaattaacta atcggatttt taaatattct gactattata taaggatatt
     6961 attagcttga atttaaaacc aataatataa aacaacaaaa tattatattt atgatataat
     7021 aaaagttatg gaaaaacagg aatattacta tagagaagag aggtaaactt atgaataatt
     7081 ggatagaagt aactataaaa acaacaacag aagcagttga acctataacg aatatactat
     7141 atgaacaagg agctggagga gctgtaatag aagacccaaa agacttttta ttccaaaaga
     7201 aaaatgaact agactgggat tatgttgaag aagaagtatt taaaaagaat gaagaagatg
     7261 atgttcttat aaaaacttat gtttctgaag aaaaaaatgt gatggaattt gtcgaaataa
     7321 taaagcaaaa agttttagga ttaaaagatt ttggtataga tataggtgaa ggaagtgtat
     7381 cattggacca agtaaatgaa gctgattggg caaatgcatg gaaagcatac tacaaaccaa
     7441 caaaagtagg acaaagggta gtagtaaagc ctacatggga agattatgct atgcaagatg
     7501 gggatttaat catagagctt gaccctggaa tggcatttgg tacagggaca catgaaacta
     7561 ctagtatgtg cattagagaa ttagaaaagt atgtgaataa agattctaaa gtatttgata
     7621 ttggatgtgg tagtggaatc cttgcaatag cagcagctaa attaggagca aaagaagttg
     7681 tagctgtaga tttagatgaa gtggctgtaa aagttgcaaa agaaaatgtt cttgaaaata
     7741 aagttgaaaa aagtgtatct gtaatgcatg gtaatttaac tgatgtaata aaagataaag
     7801 cggatgtaat agttgcaaat ataatagctg atataataaa gatacttgca aaagatgttc
     7861 aaaactttat gaaagaagat gctatattta tatcttcagg tataattttg gataaagtag
     7921 aagaagttaa agaaagctta attgaaaatg gatttgaaat agttgaagtt caaaaattag
     7981 gtgaatggtc agcaatagtt tctaagctta aaaaataagg gatatggtga cgtataatgg
     8041 atagattttt tgttgaaaaa aataatataa atctacaaga taaaacttgc actatagaag
     8101 gtgaagatgt aaaacatatt tcaaaagttt taagatgcaa actaggtgag aaacttgaaa
     8161 tatgtgacaa gaataataat gagtatattt gtgagataat gaatatagat aagagtatag
     8221 ttaatttaga gattttagaa aaagttgata taaatagaga atcagaactt aaagtaaggt
     8281 tataccaggg acttccaaaa gctccaaaaa tggaaatgat acttcaaaaa ttaactgaag
     8341 tgggtgtaga agaaataata ttagttcaaa ctaagagaag tgttgttaaa gttgatgaca
     8401 aaaaagaaga taaaaaattt gaacgttggg aaagaataat ttatgaagca gcaaagcaaa
     8461 gcaaaagagg taagatacca aaattaagag gtgttttaag ttttaaagaa gctttagaag
     8521 atatgaaaaa aaataatgta aatatttgcc cttatgaaaa tgaaagaaca gtttcaataa
     8581 aacatgcact aaaaaaatgt gattctaata tagatagtgt tggaattttt ataggtccag
     8641 aaggtggatt ttctgaagaa gaaatagagc aaatacaaaa aaataattgt aatgttgtat
     8701 cattggggcc tagaatatta agaactgaga cagcttctgt tgtagcatca acaatagctt
     8761 tgtatgaact aagtgattta ggaggagaaa aataaattga aaaaagtagc tttttataca
     8821 cttgggtgta aagttaatca atatgaaaca gaagctatgc tagaactatt tgagaaagat
     8881 ggttatgaac aagtcaatag tgaagagtat gctgatgttt atgttataaa tacatgtaca
     8941 gttactcata tgagtgatag aaaatctcgt caatatataa gacgagttaa aaagaagaat
     9001 ccagatgcaa taatagccgt tgtaggatgt tattctcagg tatctccaga agaaattttg
     9061 gatatagaag aagtaaattt agtcatgggt acaaatgata gaagaaagat tgttgaagaa
     9121 attaaaaaga taaattcaag taagaaagtt agtacagttg atgacataat gaaagttaaa
     9181 gcttttgaag aaatagaaat cagccaaact aatggaaaga ctagagcatt tatgaaaata
     9241 caagatggat gtgacagatt ttgtacttat tgtatcattc cttatgcccg aggacgcgtt
     9301 agaagtagag atattgatag tatagtcgat gaagtgaaaa aattggctaa taatggctat
     9361 aaagaggttg tattaacagg aatacatgtt gcctcttatg gaaaagattt aaaggatagg
     9421 gatattaaac ttcttgatgt aataaaacaa ataaaccaga tagaaaaaat agaaagaatt
     9481 aggcttagtt ctgttgaacc aattttattt acagatgaat ttgtaaatga agttttaaag
     9541 atggataaag tttgtcctca ttaccattta tcacttcaaa gtggatgtga tgaaactcta
     9601 aaaagaatga atagacgata cacaacttta gagtacaaaa ctattgtaga tagattgaga
     9661 agtaaaatgc cagatgtggc aataactaca gatgtaatag taggttttcc tggagagaca
     9721 aatgaagagt ttaaaaaaac gtacgagttt ttaaaagaaa tagagctttc acaaatgcat
     9781 atttttaaat attcaccaag aaaaggaact ccagctgcaa ctatggaaaa tcaagttgac
     9841 cctcaaatga aacactttag aagtgaacaa cttttaaatt taagtaaagt aaattttaat
     9901 aaatttgcaa ctaaatttat cggccgtgag ctggatgttt tgtttgaaca aaatatagaa
     9961 ggcaataaat atgagggatt aacttcaaat tatataagag ttgttgttga aagtgacaaa
    10021 aatatacaag gtcagatatt aaaagtgaaa ataaatgatg taaaagatga atatgtagaa
    10081 ggaattttgc tataacatgt agaaattata atatttataa gataggaggt ggaaagaatg
    10141 gattgtattt tttgtaaaat agcgaacgga gaaatacctt caactaaagt ttatgaagat
    10201 gatagagtct tagcttttaa tgacttgaac ccagttgctc catatcatat attagttgta
    10261 cctaaaaaac attatgatag tttaattgat attcctgata aagagatgga cattgtgtca
    10321 catattcatg ttgttattaa taaaatagct aaagagaaag gttttgacca aactggattc
    10381 agagtcataa ataattgtgg aagtgatgga gggcaagaag ttaaacattt gcactatcat
    10441 attttagctg gtaaaaagct tcctaattac gaagcgggac aaaactaaac taaattattg
    10501 aaaaattgag ttccttcttg ataaaaatac ttgataaaag taaaaattta gtttataata
    10561 aacaaagtgt taggttgtaa tgcatattat gcaaaaagaa catagttaca gtcccagcat
    10621 aattgcgcta ttggagggag ggaaaaagaa atgtcagaag taagagttag agagaatgaa
    10681 acattagaca gcgctttaag aagattcaag cgtcaatgtg caatgtctgg catcatgtca
    10741 gaagtgagaa aaagagagca ctatgataag ccaagcgtta agcgtaagaa aaaagctgaa
    10801 gcagcaagaa gaaaaaacgc taagaaatag tagtaaataa gaataaagag gtgaagggga
    10861 tgtcccttaa gcaaaagtta caagaggatt tgaaatcttc gatgaaaaac aaagatacag
    10921 tgagaaaatc tgtagtaact ttaataagag cttctataaa gcagtatgag gttgataata
    10981 gggtcgagct tgatgaagat ggaattatcg atgtaatagc aaaacaattg aagcaacgta
    11041 gagatgcatt agttgaattt gaaaaagctg gaagagaaga cttaataaaa gaaacagaag
    11101 gtgaaattga agttttaaaa gagtacctac ctcaacaatt aagcgaagaa gagttagaag
    11161 aaattgtaaa atctactata tccgaagtag gggcaacttc tatgaaggat atgggaaaaa
    11221 taatgtcagt tatccaacca aaagttaaag gaagagctga cggaaaactt ataaataagt
    11281 tggtaaaaca aaacttacaa tagattgata tagcctagag agaaatctct aggcttttta
    11341 tagtttttgg gaataacgag tcatatttga gcatatctat aatcaaagaa aattttgaaa
    11401 gaaggggaaa aatttgatta tatcagtaaa agagattagg aattttttga taaacatatt
    11461 tataccacta ataataggtt acttaagtag catcttaaca atgattatca gtggaacaga
    11521 tatttcaacc tattatttac aactcacaaa gccaagcttt gcaccaccag cttttatctt
    11581 tcctatagtt tggactatat tgtacatatt gatgggtata tcttcatacc taatattaag
    11641 aaagggttac aacttaccta aggtaagaga tgcaattttt tattatggat tacaacttgt
    11701 attaaacttt atttggagta tactattttt tggatttgga cttagattta cagctttgat
    11761 agatataata ataatgattt tggtttctat aataatgatt tcgaagtttt ctaaaataga
    11821 taagagatct ggatacataa atttgattta tttaatgtgg cttgtttatg caggattttt
    11881 gaactatttt atatggttta ttaataaata gaatgtaaag attttgtaaa ttaccctgta
    11941 cttttaataa tatcataaca aacgaaaaat aaatagacta ttaaaaataa tcattttcat
    12001 atacaataat attaatttaa atacaataaa tttataaaaa ttgtatttaa agattaacat
    12061 attaaagtat tagaattgga aaggcttgaa gggagaggtt tttatgcaaa taatagtatc
    12121 tgggagacaa atgaaattaa ctgacggaat aaaaggatac gtagatggaa aattaagtag
    12181 gttagaaaaa taccttgatc ctgaatcaga agtgaaagta acagtaagtg cgaaaaagga
    12241 tagacaaaaa gtggaggtta caataattcc tattaacggt caaataataa gagcagaaga
    12301 tgtagaagat gacttatatg cagcaattga tattgtttgt gacaaattaa gtagacaagt
    12361 tgtcaaatat aaaactaaag ttaaagacaa agttcaaaat aataagagca taagatttga
    12421 aaatcttgac tttatagata atagcagtga atttgatgat tatgattatg atgatgaaga
    12481 agatgaaaat atagtaatag aaagaagaaa gaaatttaat gtaaaaccta tgagttctga
    12541 ggaggcgata cttcaaatgg aactagtagg acacaatttc tatatgttta gaaaccaaga
    12601 taattttgaa ataaacatag tgtataagcg taaagctggt ggatatggtt taatagaaca
    12661 agattaataa aaattgtata cgtttagatt ttatataaga aaaaataaaa aggtaatcat
    12721 gttgattttt tatcaatatg attacctttt tattgtttaa ataatatatt agatattagg
//
LOCUS       DAMJWP010000094        12568 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.098, whole genome shotgun sequence.
ACCESSION   DAMJWP010000094 DAMJWP010000000
VERSION     DAMJWP010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12568
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.098"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            36..1466
                     /locus_tag="PC700_002168"
     CDS             36..1466
                     /locus_tag="PC700_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2581476.1"
                     /translation="MNLDLEFYQKILEASHDEICVSDDKGIIIYCNKAFEENYGLKKE
                     DILGKNVSFLEDSGYSTKSPIPIVLKTKTKFSLEQDTQTGKKLIITATPIFDENGNLE
                     FTVENCRDITELNNIKNKLEDTKKQVKKYKSEVETLYRTALRIEDTVIMDGIVMRPII
                     NTVNHVSKTDVSVLLLGESGTGKSSLARYIHHNSNRSNGPFITINCATISPQLLESEL
                     FGYTSGAFTGASTKGKVGLVELANGGTLFLDEIGDIPQNLQAKFLQLIQDRTFTPVGS
                     LKNKKVDIRIISATNADLVSKVKEKKFREDLYYRLNVIEIKLPPLRERRDNLVEIIKY
                     YFNRYSSDFNLNKTISKEAMDAIANYRFPGNIRELQNIIQKILLTCTDNHITIDDLPN
                     ILTKNIHITNNGNKTHISQINKVIISDSKSTNYKNKNFDTLIKEYEKNIILDAYEKFG
                     SSYKVAKHLEISQSKANRLIRKYTNT"
     gene            1666..2250
                     /locus_tag="PC700_002169"
     CDS             1666..2250
                     /locus_tag="PC700_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HDF2581477.1"
                     /translation="MLMNTHFIMAKSILDNIDENKTFFISEKNFIYGNIKPDAFSKYK
                     LKKHYLDESFNMIVSKINYLCSLNIDSLSKYFSVSRLSQELGVICHFLCDFFCVAHSE
                     RWEFKHSLNKHVTYERELGAFAKDVDLSKIKCGTINSSFEKFFTKLYSDYKKKTDYMN
                     DLKFSAYTCNSVINYILDSILSNTIKSYHIANCG"
     gene            complement(2354..3133)
                     /locus_tag="PC700_002170"
     CDS             complement(2354..3133)
                     /locus_tag="PC700_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2581478.1"
                     /translation="MIKHIFCDLDGTLYENGTITKEDIVAIEEIEKKGVQFNVATGRI
                     FKQAHNIIKDSLDMNGYYVCENGSFIYDKDYNVIFKRTIDDNLVKKVIDRFESSDAQL
                     YFKYKGDVIVGSDTTAFRHYSSDFIVDPDFEKRSSFDNLIGNIGVCSENLEELSRIEL
                     YLKSEFSEVLEIYFSGTYTLNIVPKSVSKRGSIEHVIKTLNVSPDEVATIGDSPNDIC
                     MLEGFKYSFAMSKAREDVKQSANYVVDSVKSAIDVIMEINS"
     gene            complement(3203..4459)
                     /locus_tag="PC700_002171"
     CDS             complement(3203..4459)
                     /locus_tag="PC700_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HDF2581479.1"
                     /translation="MKAEIISVGTEILLGDIVNTNSQFLAKELASLGIEVYHQSTVGD
                     NKQRLLECFDESLKRSDFVITTGGLGPTGDDMTKETAAEYFGQKLELHKPSLEVLESF
                     FVKTGKKMAENNMKQVYFPKDAIVLKNNNGTAPGAILKKDGKFIIVLPGPPREMKAMF
                     NESVKPYLQQFTNEMLVSKTLRLYGIGESNLELEILDIINEQTNPTVALYAKELEVTI
                     RITAKAENEREAFKLIKPVEEKIKSRVGKYVYTEGDISISEGETALEDAVSKLLVEKN
                     LTIAVAESCTGGLVSSSLINYPGISSVFLEGCVTYSNDSKMKRLGVKRETLEEFGAVS
                     EQTAIEMAEGVAKGLKANIGISTTGVAGPGGGTKEKPVGLVYTAIYINGKTIVKKNIF
                     NGDRRKIRLRATRDLLNELRIQLEKL"
     gene            4717..5616
                     /locus_tag="PC700_002172"
     CDS             4717..5616
                     /locus_tag="PC700_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDF2581480.1"
                     /translation="MKIAVIMGGISSEREVSLNSGKEIYNNLDKNKYEVVKVIIDDKK
                     DIFTKIPEDIDFAILALHGKFGEDGCIQSILETMDIPYSGCGPLCSGMCMDKNITKKM
                     LRDSNLPTAPWVLVKSVDEIDYDEIDNIGYPVFIKPNSGGSSVATFFIHSKDEVEEAV
                     RKGLEVDEFVMIEKYIPGGEYTSFILNGEVFPTISIKSDSGFFDYEAKYSVEKGAKEE
                     VVYLDEELQKRVNEISETCWKIFNCKAYVRVDMIISEGIPYVLELNTLPGMTQTSLIP
                     RSAAARGIKYSELLDKLIEYSLN"
     gene            5640..7088
                     /gene="ddlR"
                     /locus_tag="PC700_002173"
     CDS             5640..7088
                     /gene="ddlR"
                     /locus_tag="PC700_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DdlR"
                     /protein_id="HDF2581481.1"
                     /translation="MIFSNLKLNDNEPIYIQLKNYISDMISKGLIPDNSKLPSTRELS
                     QLLQVSRNSVVLTYEELKSEGLIYSISGKGTFVKSKNKSSNTTWSLNWDCLENTYSKK
                     ANELDIIKSEIPWSSDLISFKSISPDGDLFDMEELKKSFLNRISLEGHKLLNYGYAQG
                     YKPLIDYLLEYMTNKGADISNKDILITNGFTEGFDIIMSSFTQEKDYIICENPTHNTA
                     IKIMKSHNLNILGVDINEDGLDFNMLEDNLVKYKNKIKFAYITPSYHNPTGIVMTPEN
                     RYKFYNLMKKYNIAIIEDGFNEELLYNSSHIFPICSLDNMNNGVVYIGSFSKILFPGM
                     RIGWIFADKKVINKLESVKRCRNIHVSFLDQGILFDYLSNGGFEKYIKKIRKFYGDKF
                     NFAYKCIKKYIKTEYILGDGGLHIFIKLKNIDTRVLLEKCYEKNVIFMPGDLFFTDNS
                     GFDTLRLGFSRLSFEDIEIGIKIIGETINEMF"
     gene            7147..7953
                     /locus_tag="PC700_002174"
     CDS             7147..7953
                     /locus_tag="PC700_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF2581482.1"
                     /translation="MNLILGDNMSKKSKRRKINKLKLTIVITVLFLALLSIYEILFSN
                     PNKKGNIKSQETMQNKNNTTDDSKNNQSSTKKQEDNNIKPTFSYSSIPDNIKNKMIGK
                     SMPTDEPISFDSLSYLKLTYYGFDEKTHQGEMIVNSELAPEVVDIFKELYEKKYPIEK
                     IKLIDDYDAVDEKSMSDNNTSSFCYRTIAGTNVVSNHGKGRAIDINPLQNPQVSGNDV
                     TPKISTVYADRSSTKFGMIKKGDDCYNAFVSRGWSWGGYWKNPDYQHFEK"
     gene            7979..9898
                     /locus_tag="PC700_002175"
     CDS             7979..9898
                     /locus_tag="PC700_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071223118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF2581483.1"
                     /translation="MNLMTLENISKSYSEKKLLENISLGINEGEKIGLIGVNGTGKST
                     LLKIIAGVEESETGNITKTNGIRIEYLPQNPTYNENSTVLEQVFNGTSEELKLLGEYQ
                     EILEKLNSNFTEELNKKLLSVHEKIDALNLWDLESEAKSVLTKLGINDFNQKIAELSG
                     GQRKRVSLASALITPCEFLILDEPTNHLDNDTIDWLEEYLNSRKGSLLMITHDRYFLD
                     RVTNRIIELDKGRLFSYDGNYSIFLEKKMERLSLEASIEDKRQNLIRTELAWVKRGAQ
                     ARSTKQKARLQRFDELMNRDSFKPDENLDISVASSRLGNKIIEIHNISKSFEENTVID
                     NLEYTLTRTDRIGIIGKNGMGKSTLINILNGEIEPDSGYISIGETVKIGCFSQDDSHM
                     NIDMRAIDYVKEASDYIETSDGTRITASQMCERFLFNGTMQYTLIGKLSGGERRRLHL
                     LKILMTAPNVLLLDEPTNDLDIETLKILEEYLDEFKGVVVTVSHDRYFLDRICNKIFA
                     YEGNGKIHIYTGNYSDYLIYREIQGIEFEESKRESVKKDDSTTLKKEKPKNDKKLKFS
                     YNEQREFENIDSDIEKLESKIATLDESTAKFSTDFTKLQEILDEKSKLEKELEYKYER
                     WEYLNNLADEIANNK"
     gene            complement(10140..10487)
                     /locus_tag="PC700_002176"
     CDS             complement(10140..10487)
                     /locus_tag="PC700_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HDF2581484.1"
                     /translation="MSKSNDVLDIVFKITDGLEYEVAENSVVTILEKQDHKIQKFFRK
                     LKARIPKYKKIELDEYSSFVFLQIDGQRTVREIGKNLEAKYGEESHPLYERLLVFLNH
                     IEVNCHYIERLDI"
     gene            complement(10474..12402)
                     /locus_tag="PC700_002177"
     CDS             complement(10474..12402)
                     /locus_tag="PC700_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HDF2581485.1"
                     /translation="MNKKLPKGAYGEVSGKDYVPYITDKSRTGGNVAVLIIGIILAAI
                     FAASTTYSGMKAGLTVAAGIPGAIIGSAFVGAFARSKGILGKNLIQGMSSGGESVASG
                     FIFVLPAVILIGSQITFFEGLAVGVGGVLFGIGIAAIVHNYLIVEEHGKLMYPESMAI
                     SETLVASEAGGDSIKYMGIGFVISGFITVLTGSFLNVANNVMSLVGSKFYKWKFDIEV
                     NPLLLGIGFIVGLEVSLTMFAGSILSNFGIAPLIGYFTDMAKDGAMVWNNPAMPLNQM
                     DVGAISSSYVKYIGAGMMLCGGIIGAIKLIPTIIASIKETLKAKSSAGEGEEGSSIQM
                     ILLLGGVVIGFLAAFLISGNIVMAIIGAIISLLLSLLFVIVAGRLTGTIGTSNLPVSG
                     MTIASLVIVTLVFVIMGWTDLEANKSLLLFGSFIVVAIAIAGGYTQSQKVTYIIGGSK
                     NEMQRYFTIASIVGVIVVVGVILLLSDQLRATGDNVQFALPQANLMSTLTSGIMSGSL
                     PWVMIIVGVFMAIVLYALNLPIMTIAIGFYLPIATTSIILVGALIRLFVELVSKTEKE
                     KEVKVSNGISLSSGLVAGGSIIGLIGIILQVTGVVTPKVPSGFAATNSMAIALLVVLV
                     VLTTLPIVLSKVKNNEQE"
BASE COUNT         4766 a         1831 c         1722 g         4249 t
ORIGIN      
        1 atatatcatt aaaaatttag gaggaaaata taaaaatgaa tttagatttg gaattttatc
       61 aaaaaatatt agaagcttcc catgatgaaa tatgtgtttc tgatgataaa ggaataataa
      121 tatattgtaa taaagcattt gaagaaaatt atggtctaaa aaaagaagat attttaggaa
      181 aaaatgtttc ttttttagaa gactctggat attcaacaaa aagccccata ccaattgtat
      241 taaaaacaaa gactaaattt tcactggaac aagatactca aacagggaaa aagcttatca
      301 taactgctac ccctatattt gatgaaaatg gaaacttaga atttactgtt gaaaattgta
      361 gagatataac tgaacttaat aatataaaaa ataaacttga agacacaaaa aagcaagtaa
      421 aaaaatataa atctgaggta gaaacactat acagaactgc acttagaatt gaagatactg
      481 taattatgga cggaatagtg atgagaccaa tcataaacac agtaaatcat gtttctaaaa
      541 cggatgtaag tgtactttta ttgggtgaat ctggaacagg aaaaagctca cttgctagat
      601 atatacacca caacagcaat agatctaatg gcccatttat aacaataaat tgtgcaacta
      661 tatctccaca acttcttgaa tctgaacttt ttggatatac ttcaggtgct tttacaggtg
      721 caagtactaa aggtaaagtt gggctcgtag aattagctaa tggaggaact ctttttttag
      781 atgaaatagg agatatacca caaaatctac aggcaaaatt tttgcaatta atacaggata
      841 gaacttttac tccagttggg agcttaaaaa ataaaaaggt tgatataaga attatatctg
      901 ctacaaatgc tgatttagtt tcaaaagtaa aagaaaaaaa gtttagagaa gatttatact
      961 atagattaaa tgttatagaa ataaaattgc ctcctcttag agaaagaagg gataatctag
     1021 tggaaattat aaaatattat tttaataggt actcaagtga cttcaaccta aataaaacta
     1081 tttctaaaga agctatggat gctattgcaa attatagatt cccaggcaat ataagagaat
     1141 tacaaaatat aattcaaaaa atcttgctta cttgtacaga taatcatata acaatagatg
     1201 acttaccaaa cattcttaca aaaaatatac atattaccaa taatggaaat aaaacacata
     1261 tatcccaaat taataaggtt attatatctg actcaaaatc tactaattat aaaaataaaa
     1321 attttgacac cttaattaaa gagtatgaaa aaaatataat tttggatgct tatgaaaaat
     1381 ttggtagttc ttataaagta gccaaacacc ttgaaatctc acagtctaaa gccaataggt
     1441 taattagaaa gtatactaat acttaatttt aaatttgata aataaaaact taacactatt
     1501 tacatattgt aaatttggcc atttactgtt attatgaata tggttgaaat tatttaaata
     1561 aatactgttg acttagtttc atatatttac cattaaactt ttatcataaa tatcatatca
     1621 taataatata tttaataagt ctaataaact atacgaggtg taaatatgtt aatgaatacc
     1681 cattttatta tggcaaaatc aatacttgat aatatagatg aaaataaaac attctttata
     1741 agcgaaaaaa attttatata tggcaatata aagcctgatg ctttttctaa atataaatta
     1801 aaaaaacatt atttagatga gtcatttaat atgattgttt ctaaaataaa ctatctatgc
     1861 agtcttaata tagatagtct ttctaaatac ttttcagtaa gtagacttag tcaggaacta
     1921 ggagtcatat gtcatttttt atgtgatttt ttctgtgtag ctcacagtga acgttgggaa
     1981 tttaagcata gcttaaataa acatgttact tatgaaagag aacttggagc atttgcaaaa
     2041 gatgttgatt tatctaaaat taaatgtggt actataaata gtagttttga gaagtttttt
     2101 actaaacttt atagtgatta caaaaagaaa actgattata tgaatgattt aaaattttca
     2161 gcttatactt gtaatagtgt cataaattat atacttgact ctattttatc taatacaata
     2221 aaatcatatc atatagcaaa ttgcggttaa cttaagaatt tttaaattta tataaactta
     2281 taattgaata taattaaaat gactgctcta tagaatataa ttttatactt ctctaggaca
     2341 gtcatttttt atactaacta tttatctcca ttataacatc tatggcactt tttacactat
     2401 ctactacata atttgcactt tgctttacat cttcccttgc cttagacatt gcaaaactat
     2461 atttaaatcc ttctaacatg caaatatcat ttggagaatc cccaatagta gcaacttcat
     2521 ctggacttac atttaacgtt tttattacat gctctatact tcctcttttt gacacacttt
     2581 taggaactat atttaaagta tatgttccag aaaaatatat ttctaatact tcactaaact
     2641 cactttttag ataaagttct attctggata actcttccaa attttcagaa catacgccta
     2701 tatttcctat aagattatca aaactacttc ttttttcaaa atctgggtca actataaagt
     2761 cactagaata atgtctaaaa gcagtggtat cactacccac aattacatca cccttatatt
     2821 taaaataaag ttgtgcatca cttgattcaa atctatcaat gacttttttt actaaattat
     2881 catctatcgt tcttttaaaa attacattat aatctttatc atatataaat gaaccatttt
     2941 cacatacata atatccattc atatctaaac tatctttgat aatattatgt gcttgtttaa
     3001 aaattctacc tgtagcaaca ttaaactgta ctcccttttt ttctatttct tcaattgcta
     3061 ctatatcttc ttttgttatg gttccatttt catatagagt tccatctaaa tcacaaaata
     3121 tatgttttat cataaattaa aagcctccaa aaacaatttt atattatagt aacacttcaa
     3181 atatatccaa tatcataaca acttaaagct tttctaattg tattctcaat tcattaagta
     3241 aatctctagt agctcttaat cttatctttc ttctatcccc attaaatata tttttcttta
     3301 ctattgtttt tccatttata tatattgcag tataaactag tcccacaggc ttctctttgg
     3361 ttcctccacc aggtccagct actccagttg tggatatacc tatattagct ttaagtcctt
     3421 tagcaacgcc ctcagccatt tctatagctg tttgctcact tactgctcca aactcttcca
     3481 aagtttctct ttttactcct aatcttttca ttttagaatc attagaatat gttacacatc
     3541 cttcaagaaa tacagaagat attcctggat agtttataag agatgaagaa accaaacctc
     3601 cagtacaaga ttcagccact gctattgtta gatttttttc tacaagtaac ttagaaacag
     3661 catcttctaa agcagtctcc ccttcagata tggaaatatc tccttcagta tatacatatt
     3721 taccaactct actttttatt ttttcttcta ctggttttat taatttaaaa gcttctcttt
     3781 cattttcagc ctttgcagtt attcttattg taacttctaa ttcttttgca tataaagcaa
     3841 ctgttggatt agtctgctca ttaattatat cgagaatttc taattctaga tttgactcac
     3901 ctattccata taatcttaaa gtcttagata ctagcatttc atttgtgaat tgttgtaagt
     3961 aaggtttgac actctcatta aacatagctt tcatttctct tggaggtcca ggcaatacta
     4021 ttataaattt accatctttt tttaaaattg cccctggagc tgtaccatta ttatttttta
     4081 gtactatagc atcttttgga aagtacactt gtttcatatt attttcggcc attttttttc
     4141 ctgttttaac aaagaaagat tccaaaacct ctagtgaagg tttatgtagt tctaatttct
     4201 gaccaaagta ttctgcagca gtttcttttg tcatatcatc accagttggc ccaagaccac
     4261 ctgtagtaat tacaaaatca cttctcttta agctttcatc aaaacattct aaaagtcttt
     4321 gtttgttatc tccaacagta ctctgatggt atacttctat tcccaatgat gcaagttctt
     4381 tagctaaaaa ttgagaattt gtatttacta tatctcccaa taatatttct gttcctacac
     4441 ttattatttc agctttcaaa atttatcact ccttgattta tagccattaa gactagtctt
     4501 aagttcataa caataatttt agcaaaaata ataacataaa taaagttaaa aaatcattta
     4561 aacatatctt taaactagat tattattcta aaaccacaca taatataaac ttcgtatata
     4621 gtggtacttt catcctcaac tgttaattca tataattaag tatgtaaata aaaagtttac
     4681 tttaaaataa taaaaattaa aataggagtg tataatatga aaatagcagt tataatgggt
     4741 ggaatatcat cagagagaga agtttcttta aattcaggaa aagagatata taataacctt
     4801 gataaaaata aatatgaagt tgtaaaagta ataatagatg ataaaaaaga tatatttaca
     4861 aagataccag aagatataga ttttgcaata cttgctttac atggaaaatt tggtgaagat
     4921 ggatgtattc aatcaatact tgagactatg gatataccat actctggatg tggacctctt
     4981 tgcagtggga tgtgtatgga taaaaatata actaaaaaaa tgcttagaga ttcaaaccta
     5041 cctactgctc catgggtttt agtaaaatca gttgatgaaa tcgactatga tgaaatagat
     5101 aatataggtt atccagtatt tataaaacca aactcaggag gttcaagtgt agccacattc
     5161 tttattcatt ctaaggatga agttgaagaa gcagttagaa aaggtctgga agttgacgaa
     5221 tttgtaatga ttgaaaaata tataccaggt ggagaatata cttcattcat attaaatgga
     5281 gaagtgttcc ctacaatatc tataaaatct gattcaggat tctttgatta tgaagctaaa
     5341 tactcagttg aaaaaggtgc aaaggaagaa gttgtttact tagatgaaga acttcaaaaa
     5401 agagtaaacg aaatttctga gacttgttgg aaaattttta attgcaaagc atatgtaaga
     5461 gttgatatga ttataagtga aggtattcca tatgttcttg aactaaatac tcttcctgga
     5521 atgactcaaa ctagtttaat tcctagaagt gctgctgcta gaggtattaa gtacagtgaa
     5581 ctattagata aacttataga atactcttta aattagatga aataatttga ggtggtaatt
     5641 tgatattttc aaatctaaaa ctaaatgata atgaaccaat ttatatacaa cttaaaaatt
     5701 atataagtga tatgatatca aaaggactaa ttccagacaa tagtaagctc ccttctacaa
     5761 gggagcttag tcagctttta caagttagcc gaaattctgt agttttaact tatgaagaat
     5821 taaaatcaga aggattaatc tactctattt caggaaaagg tacatttgtt aaatcaaaga
     5881 ataagtcttc aaatactaca tggtcattaa actgggattg tttagagaat acatactcta
     5941 aaaaagccaa tgaattggac attataaaaa gtgaaattcc ttggagctct gacctgattt
     6001 cctttaaaag tatttctcca gatggagatt tattcgacat ggaagaatta aaaaaatcct
     6061 ttttaaatag aatttcacta gaaggacata aattactcaa ctatggatat gcacaaggtt
     6121 ataagcccct tattgactat ttactagaat atatgacaaa caagggtgct gatataagta
     6181 ataaagatat actaataaca aatggattca cagaaggctt tgatattatt atgtcatctt
     6241 ttacccaaga aaaagactac ataatatgtg aaaatcctac tcataatact gctataaaaa
     6301 ttatgaaatc tcataacctc aatatcttag gtgtagatat aaatgaagat ggtttggact
     6361 tcaatatgct agaagacaat ttagttaagt ataaaaataa aataaagttt gcatacatta
     6421 caccctctta tcacaatcct acaggaattg taatgactcc tgaaaatagg tacaaattct
     6481 ataatcttat gaaaaaatac aatatagcaa ttatagaaga tggatttaat gaagaattac
     6541 tctacaatag ttctcatata tttcctatct gttccctaga taatatgaat aatggggtcg
     6601 tatatattgg tagtttttca aaaattttat ttcctggaat gagaattgga tggatatttg
     6661 cagataaaaa agtaatcaat aaactagaaa gtgtaaaaag gtgcagaaac atacatgtat
     6721 cctttttaga ccaaggaatt ttatttgatt atttaagtaa tggaggtttt gaaaaataca
     6781 ttaaaaaaat acgtaaattc tatggggaca aatttaactt tgcatataaa tgtataaaaa
     6841 aatatataaa aacagaatat atcttgggtg atggaggttt gcatatattt ataaaactaa
     6901 aaaatataga tactagagtt ttattagaaa aatgttatga aaaaaatgtt atatttatgc
     6961 ctggagattt attttttaca gataactctg gttttgatac tttaagactc ggattttcta
     7021 ggttgtcttt tgaagacatt gaaattggaa taaaaataat tggagaaact ataaatgaaa
     7081 tgttctaggt taactaaata ttgttagcct agtttatttt tatgaggtat aatatatata
     7141 gtttatatga atctaatact gggggataat atgagtaaaa agtcaaaacg aagaaaaatt
     7201 aataaattaa aattaacgat tgtaataact gtattatttc ttgctttatt atctatatat
     7261 gagatactgt tctctaatcc caacaaaaaa ggtaatataa agagtcaaga aactatgcag
     7321 aataaaaata atacaacaga tgattctaaa aataatcaaa gctctaccaa aaagcaggaa
     7381 gataataata tcaagcctac tttttcatat tcttcaatac cagataatat aaaaaataaa
     7441 atgattggta aatctatgcc tactgatgaa cctataagct ttgatagtct atcttattta
     7501 aaacttacat attatggatt tgatgaaaaa actcatcaag gagaaatgat agtaaacagt
     7561 gaactagctc cagaagtagt ggatattttt aaggaattat acgaaaaaaa atatccaatt
     7621 gaaaaaatta aattaataga tgactatgat gctgtagatg aaaaatctat gtctgataat
     7681 aatacctcat cattttgtta tagaactata gctggtacaa atgtagtttc aaatcatggt
     7741 aaaggacgtg ctatagatat aaacccatta cagaatcctc aagtttctgg aaatgatgtt
     7801 acacctaaaa taagtacagt ttatgctgat aggtctagca caaaatttgg aatgataaaa
     7861 aaaggtgatg attgttataa tgcttttgta agcagaggtt ggagttgggg tggctattgg
     7921 aaaaacccag attatcaaca ttttgaaaaa taatagatta gaaataaagg tgaattatat
     7981 gaatttaatg acattggaaa atataagtaa aagctattct gaaaaaaaat tactagaaaa
     8041 tatatctctt ggaataaatg agggggaaaa aataggactt attggtgtaa atggtactgg
     8101 taaatctacc ttactcaaaa ttattgcagg agttgaagaa agtgagactg gtaatataac
     8161 aaaaactaat ggtattagga tagaatatct acctcaaaat ccaacttaca atgaaaattc
     8221 aactgtactt gaacaagtat ttaatggaac ttctgaagaa ttaaagctac ttggtgaata
     8281 tcaagaaatc ctagaaaaac ttaattcaaa ctttacagag gaattaaata aaaaattatt
     8341 atcagtacac gaaaaaatag atgccttaaa tctttgggat ttagaaagtg aagctaagtc
     8401 agtacttaca aagcttggta taaatgactt taatcaaaaa atagcagaac tttctggtgg
     8461 gcaaagaaag agagtctctc tggcatcagc ccttattaca ccttgtgagt ttttgatact
     8521 agatgagcct acaaaccatc tagacaatga tactatagat tggcttgagg aatatcttaa
     8581 ttcaagaaaa ggctcattgc ttatgataac tcatgataga tactttttag accgtgttac
     8641 aaatagaatt attgaactgg acaagggtag attattcagt tatgatggga attattcaat
     8701 atttttggaa aagaaaatgg aaagactttc gctagaagca agtattgaag ataaaagaca
     8761 aaatttaata agaacagagc ttgcgtgggt aaaacgagga gctcaggctc gtagcactaa
     8821 acaaaaagct cgtttacaaa gatttgatga acttatgaat agagattctt ttaaacctga
     8881 tgaaaatctc gatatatctg ttgcatcaag tagacttgga aataaaataa tagaaataca
     8941 caatatatct aaatcatttg aagaaaatac tgttatagat aatttagaat acactttaac
     9001 tcgtacagat agaattggta taataggcaa aaatggtatg ggtaaatcta cccttataaa
     9061 tatacttaat ggagaaatag agcctgatag tggatatatt tcaattggag aaacagttaa
     9121 aataggttgt ttttctcaag acgactctca tatgaatatt gatatgaggg ctatagacta
     9181 tgtaaaagaa gctagtgact atatagaaac ttcagatggt actaggatta cagcttctca
     9241 aatgtgtgaa agattccttt ttaatggtac tatgcaatat actttgatag gtaaattatc
     9301 tggtggtgaa agaagaagac tacatcttct taaaatcctt atgacagctc ctaatgtgct
     9361 tcttttagat gaacctacaa atgatttaga tattgaaacg cttaaaatat tagaagaata
     9421 cctagacgaa tttaaaggtg tagtagttac agtatcacat gatagatatt tcttagatag
     9481 aatttgtaat aaaatatttg catatgaagg taatggaaaa attcatatct atacaggtaa
     9541 ttacagtgat tatcttattt atagagagat tcaaggaatt gaatttgaag aatctaaaag
     9601 ggaatcagtt aaaaaagatg actctactac cctaaaaaaa gaaaaaccta aaaatgataa
     9661 aaagcttaag ttctcatata atgaacaaag ggagtttgaa aatattgact ctgatataga
     9721 aaaactcgaa tctaaaattg caacacttga tgaaagtact gcaaaatttt caactgattt
     9781 tactaaactt caagaaattt tagatgagaa atctaaacta gaaaaggaac ttgaatacaa
     9841 atatgaaaga tgggaatatt taaataatct agctgatgaa atagctaata ataagtgata
     9901 ttaaaataat cttaagttta ataagttttg ctttaaatct atactttata aatattttaa
     9961 aggtagtgtc tcaaaatgaa ctttttagtt ttatgagaca ctctttttta tgagaaatca
    10021 aatatgtttt catcatttca acaatacaaa agaggcttat ctctattttg agacagcctc
    10081 tttcataatt accttctaat ttatatgctt taatatatct ataataattc ttattatatc
    10141 tatatatcca gtctttcaat atagtgacaa ttaacttcaa tatgatttaa aaatactaac
    10201 aacctttcat aaagtggatg agattcttct ccatatttag cttctaggtt cttgcctatt
    10261 tctctaacag tcctttgacc atcaatttgt aaaaaaacaa aactggaata ttcatctagt
    10321 tctatttttt tgtatttagg tattctagct tttaactttc taaaaaactt ttgaatttta
    10381 tggtcctgtt tttcaagtat agtaacaaca ctattttcag ctacttcata ttcaagtcca
    10441 tcagtaattt taaatacaat atctaaaaca tcattactct tgctcattat tttttacttt
    10501 agataaaaca ataggtaatg ttgtaagcac aactaaaact actaataaag caattgccat
    10561 tgaatttgtt gcagcaaatc cacttggtac ctttggagtt acaactccag ttacttgtaa
    10621 tattatacct attaaaccaa tgatagaacc accagcaaca agaccagaag ataaacttat
    10681 accattagaa actttaactt ctttttcttt ttcagtttta gatactaatt caacaaataa
    10741 acgaattaat gctccaacta atattataga agttgttgca attggtaaat agaatcctat
    10801 agctatagtc attattggta aatttaatgc atatagaact attgccataa atactccaac
    10861 aataatcata acccaaggta aactacctga cataatacca gaagttaatg ttgacattaa
    10921 gtttgcttgt ggtaatgcaa attgtacatt atcaccagtt gctctaagtt ggtcagaaag
    10981 taataaaatt actcctacaa caactataac accaactata cttgcaattg taaagtaacg
    11041 ttgcatttcg tttttgctac caccaataat ataagttact ttttgagatt gagtataacc
    11101 tccagctata gcaatagcaa caactataaa tgaaccaaat aaaagtaatg atttattagc
    11161 ttctaaatca gtccatccca ttataacaaa cactaatgtt acaataacta gagaagctat
    11221 agtcatacca gaaactggaa gatttgatgt tccaattgtt cctgttaaac gaccagctac
    11281 tatgacaaat aataaagata ataaaagaga tatgatagct ccaataattg ccataacaat
    11341 gttaccagaa attaagaatg ctgctaaaaa tccaataact actccaccta ataaaagtat
    11401 catttgtata gatgaacctt cttctccttc tccagcactt gatttagctt taagtgtttc
    11461 ttttatagaa gcgattatag ttggtataag ttttattgca ccaataatac ctccacaaag
    11521 catcatacca gcacctatat attttacata actactagat attgctccga catccatttg
    11581 gttaagaggc attgcaggat tattccatac catagctcca tctttagcca tatctgtaaa
    11641 gtatcctatt aaaggtgcta taccaaagtt tgataaaata cttcctgcaa acattgttaa
    11701 agaaacttca agccctacta taaatccaat tcctaaaagt agaggattta cttcaatatc
    11761 aaatttccat ttgtagaatt tgcttcctac aagactcatt acattgtttg ctacatttaa
    11821 gaatgaacca gttaatacag taataaatcc acttattaca aatccaattc ccatgtactt
    11881 aattgaatct ccaccagcct ctgaagcaac aagtgtctca gatatagcca ttgattctgg
    11941 atacataagt ttaccatgtt cttcaactat tagatagttg tgaactatag cagcaattcc
    12001 tataccaaat aaaactccac caactccaac agctaggcct tcaaagaatg taatttggct
    12061 tccaattaat ataactgctg gtaatacgaa aataaatcca cttgcaacag attctccacc
    12121 acttgacatc ccttggatta agtttttacc aagaataccc tttgaacgag caaatgctcc
    12181 aacaaaagca gaacctatta tagctcctgg aatacctgca gcaactgtaa gaccggcttt
    12241 cattccagag taagttgtag atgcggcaaa aatggcagct aaaataatac cgataatcag
    12301 tacagctaca tttccaccgg ttctggactt atctgtgata tatggaacgt aatcttttcc
    12361 actaacctca ccataagcac ccttaggtaa ttttttgttc ataaaatttt cacccctttt
    12421 caatttgtat atattataac atatatacat ataaaactta actttttatt attttacaaa
    12481 tagcgagtca aaactactaa aatttaaccc tatattatta atttctactt ttagatattt
    12541 ttatgctaat tttcttttta tttttcaa
//
LOCUS       DAMJWP010000095        12314 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.099, whole genome shotgun sequence.
ACCESSION   DAMJWP010000095 DAMJWP010000000
VERSION     DAMJWP010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12314
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.099"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            63..266
                     /locus_tag="PC700_002178"
     CDS             63..266
                     /locus_tag="PC700_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2581486.1"
                     /translation="MISYAPLWKLLIDRKIKKMEFVNISGISISVLGRLGNDKSVSMD
                     TMEKICLALDCKIEDVVEIKKGI"
     gene            430..1584
                     /locus_tag="PC700_002179"
     CDS             430..1584
                     /locus_tag="PC700_002179"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HDF2581487.1"
                     /translation="MKKISILGSTGSIGKQTLDVVREHRDKFEIVAISANSNIELLLE
                     QIVEFKPKYVTVFEENKALKLKEMLPKNIEIEVLAGMEGLKIISSLDEVDVLLTAVVG
                     MIGLVPTLCAIKKGIDIALANKETLVTAGELVMKEAEKYNVNILPVDSEHSAIFQCLN
                     GENKKNIEKIILTASGGPFRGKKKGELVNITKNEALKHPNWSMGRKISIDSSTLMNKG
                     LEVIEARWLFRVEQENIDVVVHPQSIIHSMVQYADSSIIAQLGCPDMRLPIQYALTYP
                     DRMESSFERMNFSKFSTLTFEEPDLETFPCLKLAYECLKMGGTYSSVLNSANEVLVSE
                     FLEDKIGFYDIPYYIEKTLEVHSSISEPTLEEILETDRWSRAYVANLIKK"
     gene            1597..2601
                     /gene="rseP"
                     /locus_tag="PC700_002180"
     CDS             1597..2601
                     /gene="rseP"
                     /locus_tag="PC700_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HDF2581488.1"
                     /translation="MTIIAALILFSIIVLIHELGHFIFAKRSGIKVNEFSIGMGPKIY
                     SVKKDTEYSIRALPIGGYVSMEGEDEEQISPNSFGNKSILQRFSTIVAGPIFNIILAA
                     ILLVPVFLYIGSPTTKLGKIMPDTPAQAVGLQVGDKINKINGNSVKTWDEVANIINTS
                     SGGELKLSITRDGSNKVVNVTPKNNNGKYEIGIQPQREKDFLASIVNACKTTVDMTKQ
                     MLTFLGQMITGRVPGGIGNAVAGPVGVIGMVSDAARTGIINVVYLAAVISLNLGIVNL
                     LPIPALDGWRILMLLLEAVRGGKKLDPNKEGMINVVGFGALMLFMLFITYKDILRLFQ
                     "
     gene            2784..3839
                     /gene="ispG"
                     /locus_tag="PC700_002181"
                     /gene_synonym="gcpE"
     CDS             2784..3839
                     /gene="ispG"
                     /locus_tag="PC700_002181"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HDF2581489.1"
                     /translation="MSYERRKTREVSVGTVKIGGENPISIQSMTNTDTRDADATIAQI
                     KRLEEAGCDIVRVAVPDIKAAKNIAKIKSSVNIPIIADIHFDYKLALEAIEQGVDGIR
                     INPGNIGSRERVKMVVEKCKERNLKIRIGVNGGSLEKELLKKHGSATAEALVESAMGH
                     IKILEDLDFHNIVISLKSSDIYKTVDAYELISKKVDYPLHIGITESGSVHKGTIKSSI
                     GVGALLLKGIGDTVRISLTGDPVEEVIVGKQILRSLGLLNDKIKVISCPTCGRCNIDL
                     ISVVNEVEEKIGSMEKNITVAIMGCAVNGPGEAREADIGIAGGKGEGLLFKKGEIVRM
                     IDGNKLVDELLEEIEKL"
     gene            4187..5011
                     /locus_tag="PC700_002182"
     CDS             4187..5011
                     /locus_tag="PC700_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581490.1"
                     /translation="MRYKKISSLPILVVALLITGTTTTFSLSTTNSEKTNVYLASTVS
                     TVTKKVSDIQSSKGLVYNENIPLFINNSKIQYDDTPYHWPSNVISLTNSSEKAIMDYE
                     ITCLAYDKNGKPLELYWDAQNVAADGEVGSVGFSPAGVDYGIVTGISPVSPKSYSHTY
                     RKMQQSPPQDIISMFEKQQGKAWVENWLKEWKQMEKEYAKQNAIAPGKNQNDAFLLFD
                     KWKQSTGEHGVKYIISCVKQVTFNDGSIWKNSAYENWLKSFQGKEVSNSVLENYYK"
     gene            complement(5484..6607)
                     /locus_tag="PC700_002183"
                     /pseudo
     CDS             complement(5484..6607)
                     /locus_tag="PC700_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
     gene            6804..7028
                     /locus_tag="PC700_002184"
     CDS             6804..7028
                     /locus_tag="PC700_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581491.1"
                     /translation="MIIKLTKDNFNFRFKQYEGMFFHAEVVEGMESIRYKITKDKEGT
                     ELKEFGSTHIKDNMIYVPQVEAYIDLNSIK"
     gene            7081..7605
                     /locus_tag="PC700_002185"
     CDS             7081..7605
                     /locus_tag="PC700_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2581492.1"
                     /translation="MSKPIDITIRPIRREDAQAFNEIRRTYSVMRNTLALMSDRVDKT
                     EAMLASLGENDYMFVAETNNNGEKDVIGFAGLHVNSSPRLRHSAEIGISVAENYQGRG
                     VGKKLMEQLLDIADNWIMLTRVGLEVIVDNEKGLNLYKKLGFEIEGTKKYAVIRDGKF
                     EDVYIMGRYNKNLI"
     gene            complement(7710..7856)
                     /locus_tag="PC700_002186"
     CDS             complement(7710..7856)
                     /locus_tag="PC700_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581493.1"
                     /translation="MDMDLLFKIVFAIVAIGVSVKIIKTVASLAFKIALILLIILFVY
                     RMFL"
     gene            complement(7995..8921)
                     /locus_tag="PC700_002187"
     CDS             complement(7995..8921)
                     /locus_tag="PC700_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDF2581494.1"
                     /translation="MNFSNIFIQVAVLFIIILVGYFVRKFNLLDDHCTSKLSTLTMTV
                     FLPSMIISSMQINFDSKMIQKILLLLFTSLIMYIVSIIIAFLLKYILKCNDKKDLGIY
                     QYIVVFSNVAFMGYPVIEAVLGHEAIFYTAIFNLPFNLFSFTLGIYLLSKGSTSRGFS
                     IKSLISPATIAIVIGLFLFVTGLRLPQFINEPLEMLGSITTPISMIIIGSLLANSSAL
                     DCFVNKKLYIVTFIRLLVLPVIVYFILKGWINDKMILAIPVVISSMPAAANTAIMANQ
                     YDSNITLASQCVFFTTLFSVISIPFISILLLS"
     gene            9112..10983
                     /locus_tag="PC700_002188"
     CDS             9112..10983
                     /locus_tag="PC700_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDF2581495.1"
                     /translation="MKNTKFSIKKIKNISNDPKVVHTKGALLEKMGKMESAIELYKSA
                     ALDNYVKSQYTLGNIYESKKQFKEAEKWYSMAYKSGSEDAAFDLGNMYYKLDGYEYAI
                     YWYEKIANLGYLQAQNNLGVCHFKMKDFVRCEKWLKTAADKNLGKACFNLGVLYTFLE
                     KDDEAYEYYKKGSVLLDDDAKYNLAILNGKKKDNKSTVSLYKQLYKSGHMKGCFNLGM
                     IMEINDNLEDAEKYYKKSADRDDVKSEYRLAYVYDRKEELGDAIYYYEKAIEKNHTLS
                     KYRLANLYNRENEIEKAKTYYKMAAEDDITEAKNNLAGIYFEEKDYENAIKYYEDAIA
                     VGCKSSLENLGDLYYQNQDIEKAISYYSRIPNNASCQIKLGNIYEDLNNIEEAISWYK
                     KASENGDTRSSYRLGCIYESLGNTKNARKYFEMASSKNHMNARIHLGRIYFREGKLEE
                     SKMMFDTPANENNVYAQHMVGLIYDMFYKDYVNSKFWYEKARAQGCVESIYNLGQIYL
                     KLNDDAEAEKYYKEGIKYGSKKCEYMLAGLYYKKSLDMYISLANEEYDNCEEIVEGFP
                     NLNIDFDEVLIPPFKLQEEVIDEEEYVPIYILNIKESLDSMLEGLETEMIIDEEHDS"
     gene            complement(11075..12010)
                     /locus_tag="PC700_002189"
     CDS             complement(11075..12010)
                     /locus_tag="PC700_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDF2581496.1"
                     /translation="MIRYYKTIDSKLEKLSFFEDGCWINLVEPNHSEINEISNLLNID
                     VESIESALDEEERSRIDVEDNHTLILIDIPVDESDSNSSHYTTIPLGIILTEEAIVTV
                     CDAQTKILNDFIVGHIKDFFTFKKTRFLLQILHKNAAYYLHYLRKINKMTIIIEREIY
                     KSMKNKELVQLLELEKSLVYFSTSLKSNELVLNKMVRTAGIKKYPDDEDLLEDVIVEN
                     RQALDMAKIYGDILSRIMDAFSAIISNNQNNVMQILTVVTLIFSIPTIISGFFGMNVI
                     NMPFSNNPNGFWIILLITSIICIVITFFMSRNKLL"
BASE COUNT         4853 a         1297 c         2084 g         4080 t
ORIGIN      
        1 aatagatgaa aattttccaa aatagataaa atgtgataga atatattttg cgaggtgata
       61 acatgatttc atacgctcca ttatggaaat tgttaattga cagaaaaata aagaaaatgg
      121 aatttgtaaa tatatcaggt ataagtattt ctgttttagg cagactagga aatgataaat
      181 ctgtttctat ggatacaatg gagaagatat gtttagcact agactgtaaa atagaagatg
      241 ttgtggaaat aaaaaagggc atttaataat actagaatat tttataatat tccacaagat
      301 acaaaaaata atagattaat tttaatctgt ggaataatat atgaaatttc tatgaatagt
      361 aaaaataatt gttttagtag tgttataatg ttttgacagg gtatatttat tattagaaag
      421 gattattata tgaaaaagat atcaatttta ggttctacag gctctatagg taaacaaact
      481 ttggatgtgg ttagagaaca tagagataag tttgagatag ttgcaatatc agctaacagt
      541 aatattgaat tattgctaga acaaatagta gagtttaaac ctaaatatgt aacagttttt
      601 gaggaaaata aggcattgaa attaaaagaa atgttgccta aaaatataga aatagaagtt
      661 ttagcaggaa tggaaggatt aaaaataata tcatcacttg atgaagtgga tgtcctttta
      721 actgctgtag taggtatgat aggattagtc ccaactcttt gtgctataaa gaagggaata
      781 gacatagcat tagcgaataa agaaacgttg gtaactgctg gtgaacttgt aatgaaagaa
      841 gcagaaaaat acaatgtaaa tattcttcct gttgatagtg aacatagtgc tatatttcaa
      901 tgtctaaatg gagaaaataa aaaaaatata gaaaagataa tacttacagc atctggtggt
      961 ccatttagag gaaagaaaaa gggagaactt gtaaatataa ctaaaaatga agctttgaag
     1021 catccaaatt ggagtatggg aagaaaaata agtattgatt cttcaacgct tatgaataaa
     1081 ggacttgaag tgatagaagc tagatggttg tttagggtag aacaagaaaa tatagatgta
     1141 gtagttcacc cacaaagcat aattcattca atggtgcagt atgcagatag ctcaataatt
     1201 gctcaattag ggtgtccaga tatgagacta ccaatacagt atgctcttac atatccagat
     1261 agaatggaaa gtagctttga aagaatgaat ttttcaaaat ttagtacttt aacttttgaa
     1321 gaaccagatt tagaaacttt tccatgttta aaattggcct atgagtgttt aaaaatggga
     1381 ggaacttatt cttctgtttt aaattcagca aatgaagttt tagtaagtga atttttagag
     1441 gataaaattg gtttttatga cataccatat tacatagaga agactttaga agttcatagt
     1501 agcataagcg aaccaacatt agaagaaatc ttagaaacag atagatggag tagagcttat
     1561 gttgcaaatc tgataaaaaa ataggaaggt gaatttatga ctataatagc cgcattaata
     1621 ctatttagta taattgtatt aatacatgag ttaggacatt ttatatttgc aaaaagaagt
     1681 ggtatcaaag taaatgagtt ttctataggt atggggccta agatatatag tgtaaaaaaa
     1741 gatacagagt attctataag agcacttcca attggtggat atgtcagcat ggaaggtgaa
     1801 gatgaagaac aaataagtcc aaattctttt ggaaacaagt ctatattaca aagatttagt
     1861 actattgtag caggaccaat attcaatata atattagcag caatactttt agttccagta
     1921 tttttgtata taggttctcc aacgactaaa ttaggaaaga taatgccaga tactcctgca
     1981 caggctgtag ggcttcaagt aggagataag ataaataaaa ttaatggtaa ttctgttaag
     2041 acttgggatg aagttgcaaa tataataaac acttcttctg gtggagaatt aaaacttagt
     2101 ataactagag atggaagtaa taaagttgta aatgtaacac ctaaaaataa caatggaaag
     2161 tatgaaatag ggattcaacc acaaagagaa aaagactttt tggcgtcaat agtaaatgca
     2221 tgtaaaacta ctgtagatat gacaaaacag atgttaacat ttctaggtca aatgattaca
     2281 ggacgtgtac caggagggat tggcaatgct gtagcaggac cagtaggtgt tattggtatg
     2341 gtatcagatg ctgctcgtac aggcatcata aatgtagtgt atttagcagc tgtcataagc
     2401 ttaaatttag gtatagtaaa tctattacca ataccagcac ttgatggatg gagaatactt
     2461 atgctattac tagaagctgt tagaggtggt aaaaaactcg acccaaacaa agaaggcatg
     2521 ataaatgttg ttggttttgg agctttaatg ttatttatgc tttttatcac atataaagat
     2581 atattaagat tatttcaatg atttagtttt ttagagattt taagaaaata ggctatgtag
     2641 ttttaaataa gaataaatta atttttatgt atgaaattta ttcttattta actagatagc
     2701 tttttaattt ataatataca tgtaggttat aatattttac tatttaaaga gattcaatat
     2761 aaaataatat atagaggtgg aatatgagtt acgagagaag aaagacgaga gaagtaagtg
     2821 taggaactgt aaaaatagga ggagaaaatc ctataagtat acaatctatg acaaatactg
     2881 atacaagaga tgcagatgct acaatagctc aaataaagag actagaggaa gctggttgtg
     2941 atatagttag agttgctgtt cctgatatta aagctgctaa gaatatagca aagataaagt
     3001 ctagtgtaaa tataccaatt attgcagata tacatttcga ttataagcta gcccttgaag
     3061 ctatagaaca aggtgtggat ggaataagaa taaatcctgg taatattgga agtagagaga
     3121 gagttaagat ggtagttgag aaatgtaagg aaagaaattt aaaaattaga attggagtca
     3181 atggaggttc tttagagaaa gaacttttaa aaaagcatgg ctctgcaact gctgaagcat
     3241 tagttgaaag tgcaatggga catattaaga ttcttgaaga cttagatttt cataatatag
     3301 ttatatctct aaaatcatcc gatatttata aaactgttga tgcatatgaa ttaatatcaa
     3361 aaaaagttga ttatcctctt catattggaa ttacagaatc tggaagtgtt cacaaaggaa
     3421 caataaagtc ttctataggg gtaggagcac ttcttcttaa aggaattgga gatactgtta
     3481 gaatatcttt aacaggcgac cctgttgaag aggttattgt tggtaaacag attttaagaa
     3541 gtttgggact tttaaatgat aagattaaag ttatatcatg ccctacatgt ggtagatgta
     3601 atatagactt aattagtgtt gtaaatgagg ttgaagaaaa gattggtagt atggaaaaaa
     3661 atatcactgt tgcaattatg ggctgtgctg taaatggacc tggtgaagcc agagaagcag
     3721 atataggtat tgctgggggt aaaggtgaag gtcttttatt taaaaagggt gaaatagtta
     3781 gaatgattga tggtaacaaa ttagttgatg agttgctaga agagattgaa aaattatagt
     3841 atacaattca tatatatatt aattgactag atttttaaat acttttgtaa taagttttta
     3901 tttacagtat ttaattatct agtcattttt tattttataa acttcattaa atgtaagaaa
     3961 cttatattct aatttgacta tatatgtatg agttaaatca tattgtaaat ttataaaatt
     4021 aaaaaaagat ttaatatagt gaaaatttcc atcaatagta ttgaaacttg atagtaacta
     4081 tgataggata tagttactag aacagttggt aaattatgtt aacatagatt ttaatgtttt
     4141 ggtaaattgt tttattataa gttaatagaa aaagaggtga agaactgtga gatataaaaa
     4201 aatatctagc ttacctattt tagttgttgc tttgctgatt actggaacga caacaacatt
     4261 ttctcttagt acgaccaatt cagaaaaaac aaatgtatac ttagcaagta cggtaagtac
     4321 tgtcacaaag aaagtatccg atattcaaag tagtaaaggt cttgtttata atgagaatat
     4381 tccattattt ataaataata gcaaaataca gtatgatgat actccatatc attggcctag
     4441 caatgttata agcttaacaa atagcagtga aaaggcaatt atggattatg agattacatg
     4501 cttagcatat gataaaaatg gaaaaccact tgagctatat tgggatgctc aaaatgtggc
     4561 agcagatggc gaagtaggaa gtgttggatt tagcccagct ggtgtagact atggtattgt
     4621 tacaggcata tctcctgtgt ctccaaaatc atattcacat acttatagaa aaatgcaaca
     4681 atcaccacca caggatataa taagtatgtt tgaaaaacaa caaggcaagg catgggtaga
     4741 aaattggttg aaagaatgga aacaaatgga gaaagaatat gcaaagcaaa atgcaatagc
     4801 accaggtaag aatcaaaatg atgcctttct attgtttgat aaatggaaac aatcaactgg
     4861 agagcatggt gtaaagtaca ttatatcttg tgtaaaacaa gtaacattta atgatggtag
     4921 tatatggaaa aattcagcat atgagaattg gttgaaaagt tttcaaggga aggaagtttc
     4981 aaatagtgta ttagaaaatt actataagta atgtttaatg atagcaaaat gagtacgaat
     5041 ttaattaatg ttaatttaat taaataagga ctattttgtt taaaaatatc agtgttacaa
     5101 ggaaattttt attgtgacaa aatggtatat attcaaaata aaaaagcatg tgtcaagttt
     5161 tgatacatgc ttttttattt tgaatatatg ttttctataa atttatgatt ttttagttat
     5221 gtaattttat atgtgaattt ttagattttt atatagatag tgaaataatg tggaagtttt
     5281 atgttttaaa aattttagtt aaaacattta ttaaaaaaga taatagaaat gttttaacta
     5341 aaatagttag aattgaaatg ttatacagat agtataagta ctatcaaatt atataagatt
     5401 gcttgaagtt aaataattta tttaaataga aaacagctga tttaatatca gctgtatatg
     5461 aataaaattt tgaattgtag tgtttagtta tatggatata attcataata agttagcaat
     5521 aaatcaacca ttttaccata actctgagta ccttcagtaa cgccattagc ttttaagtaa
     5581 gaattgttaa actcattaga aatttcatta attttacctt catatttttg ccaaaattca
     5641 ctttcatttt ttaaatctct acggacagaa tctgaaatgc cagcagaaat atcaacatat
     5701 gcattataat caaccttact taaagcagaa gcagtataat ttaaagctaa aatatatcca
     5761 gaatagttaa aatcaatatg ggggtgtttt atagaagtaa gatacgctat aaaattagcc
     5821 tcatcttcac tagcaaatcc cctttgatgt gctatttcat gttcaactgt acaaggaata
     5881 tataagtcag gtattgctat atttacattt gcctcacctg taaaaggaaa gtatatacca
     5941 gttattcctg tatagcacat tagatttgat gaaataatat acttaggctt agaataacta
     6001 ccactaacac taggtaatat atccaagata ttatcgtatc ctagttgagc tctatttatg
     6061 acaccattat aatctgtatt agattttaca ataccatttt tatcttgtaa tgtgagtttt
     6121 ctagtttcat ttgctttaat tacaaggaat ttatataatt tagtaagctc tttaacacta
     6181 tattgttttg attggataga ggtattgttt tttaaattat agttatttat aagtgtggtt
     6241 tctagtggaa tcctattata gtttattccc caaagcacta caaaaagaaa gtagactatt
     6301 gatacaatgg ataatatatt taaaatggaa ttttttaaaa atatgtttaa ttttttttct
     6361 tattaaaaat tgtagataaa gtacaacata aaaataagaa gatagatata actattatgt
     6421 acattgtaat ctcatagaga gaaaatggga aaattccaga aactttactc aatatttgta
     6481 caattatttt atctatgaat tgggagtaat atttttcaac aatatttgga actttgctgg
     6541 ctataaaatt taaaagtaaa gcaataggaa agagaattaa agaaaaatat tttgtttttt
     6601 tgctcatatt tcacctcaca tacattagtt tatataaaca tattatatat caattgaaga
     6661 aagattgtaa aataagtata aatatatcta tttataactt tatactaaat aaatgataag
     6721 ttataatata ttaatatgaa taagcagtat atatacaact aataataaga aaaatatcaa
     6781 ttatataagg agtagataaa aatatgataa taaaacttac taaagacaat tttaatttta
     6841 gatttaaaca atatgaaggt atgttttttc atgctgaagt agttgaaggt atggaatcca
     6901 ttagatataa aataactaag gataaagaag gtacagaatt aaaagaattt ggaagtacac
     6961 atataaaaga taatatgata tatgtacctc aggtagaagc atacatagat ttgaacagta
     7021 taaaataaag atgtaaaatt ttaattaaaa ataaagatta tgtttgaggg ggaatccaat
     7081 atgagtaagc ctatagatat aacaataaga cctataagaa gagaagatgc acaagctttt
     7141 aatgaaatta gaagaacata cagtgttatg agaaatactt tggcattaat gagtgacaga
     7201 gtcgataaaa ctgaagctat gttagcttct ttgggagaaa atgattatat gtttgtagct
     7261 gaaactaata ataatggaga aaaagatgta ataggttttg ctgggttaca tgtaaattct
     7321 tctccacgat tgagacatag tgcagaaata ggaataagtg ttgcagagaa ttatcaggga
     7381 agaggagtag gtaagaagct tatggaacaa cttttagata tagcagataa ttggattatg
     7441 ttaactagag taggattaga agtaattgta gataatgaaa agggattaaa tttatataaa
     7501 aaactaggtt ttgaaataga gggaactaaa aaatatgctg taatcagaga tggaaaattt
     7561 gaagatgttt atattatggg aagatataat aaaaatttaa tttaaagaag ctaatagaga
     7621 agtaaaatag agtgtcttta ataaaaaatt tacattaaaa ataatctatt ttacttctct
     7681 attgtattaa aaaacttatt tttaaatgtt tacaaaaaca ttctatatac aaataaaatt
     7741 attagcaata tcaacgctat tttaaaagct aaactagcaa cagttttaat gatttttaca
     7801 gaaacaccta tagcaactat agcaaatact attttaaaga gtaaatccat atccatattt
     7861 aagcctccta ttctataata catatagata ttattgacaa atatctagga aaaaattcca
     7921 tcaatatact acaataaaat gtttatattt gtaatttatt gaatatgtta ttatataata
     7981 cataaaaaaa taaatcaaga taagagtaaa atacttataa aaggtataga tataactgaa
     8041 aatagtgttg taaaaaacac acattgtgaa gctagtgtta tattggagtc atattgatta
     8101 gccattatag cagtatttgc agcagcaggc attgaagaaa ttactactgg tatagctaaa
     8161 atcatcttat catttatcca tccttttaga ataaaataca caatcacagg aagtaccaat
     8221 aatcttataa aagtgactat atataatttt ttgttgacaa aacaatcgag agctgaagaa
     8281 ttagcaagta agcttccaat gataatcatg gatattggtg tagttatact tcccaacatc
     8341 tcaagtggct cgtttataaa ttggggtaac ctaagtcctg ttacaaataa aaaaagtcca
     8401 ataacaatag ctattgtagc agggcttata agtgatttta ttgaaaaacc tctacttgta
     8461 ctgcctttag ataaaagata aattcctaaa gtgaaagaaa ataagttaaa aggcaaattg
     8521 aatattgcag tatagaatat tgcttcatga cctaaaactg cttcaattac aggataaccc
     8581 ataaaagcaa catttgaaaa aacaactata tactgatata ttcccaaatc ttttttatca
     8641 ttacatttta aaatgtattt caataaaaat gcaattataa ttgaaactat atacataatc
     8701 aaagatgtaa ataataacaa tagtattttt tgtatcattt tactatcaaa gttaatttgc
     8761 atggagctaa ttatcattga tggtaaaaaa actgtcattg tcaaagttga aagcttagat
     8821 gtgcagtggt catctaataa gttgaatttt ctgacaaaat atccaactaa aataattata
     8881 aataaaactg caacttggat aaaaatattg ctgaaattca taaaatgtcc ccccctatat
     8941 atacattata taccaattac ataataaaat ctaatgatta taaaatttaa aatttaatgt
     9001 tacaatatag tgacaaaagg taaaaattca atattatagc attaattata gattataatg
     9061 tataataatg tttgagagaa tatattttat aaaatgggta ggtagcgaat aatgaaaaat
     9121 acaaaatttt ctataaaaaa aataaaaaat atatctaatg acccgaaagt cgtacatact
     9181 aagggtgccc tccttgaaaa gatgggtaag atggaaagcg caatagagtt gtataagagt
     9241 gctgcattgg ataattacgt aaaatctcaa tatactttag gaaatatata tgaaagtaaa
     9301 aaacaattta aagaagcaga aaagtggtat tcaatggcat ataaaagtgg aagtgaagat
     9361 gctgcttttg atttaggtaa tatgtactat aagttagatg gctatgaata tgctatatat
     9421 tggtatgaaa agatagcaaa tttaggttat ttacaagcac aaaataattt aggtgtatgc
     9481 cattttaaaa tgaaagattt tgtaaggtgt gaaaaatggt taaaaacagc agcagacaag
     9541 aatttaggta aagcttgttt taatttaggg gtattatata cttttttgga gaaagatgat
     9601 gaagcatatg aatactataa aaaaggttct gtgcttttag atgatgatgc aaaatataat
     9661 ttagcaatat taaatggaaa aaagaaagat aataaatcaa cagtaagttt atataaacag
     9721 ttgtacaagt caggtcatat gaaaggttgc tttaacttgg gtatgattat ggaaataaat
     9781 gataatttag aagatgcaga gaagtattat aaaaagagtg ctgatagaga tgatgtgaag
     9841 tctgagtata ggcttgcata tgtatatgat agaaaagaag agctgggaga tgcaatttat
     9901 tattatgaga aagcaataga aaaaaatcat acactttcta agtatagact tgctaattta
     9961 tataatagag aaaatgagat agagaaggca aaaacttatt ataaaatggc tgctgaagat
    10021 gatataacag aagcaaaaaa taatctggca ggtatatatt ttgaagaaaa agattatgaa
    10081 aatgctatta aatattatga ggatgctata gctgttggat gcaaaagttc ccttgaaaat
    10141 cttggggatt tatattatca gaatcaagat atagaaaaag cgatttcgta ttattcgagg
    10201 attccaaata atgcatcttg tcaaatcaaa ttaggaaata tctatgaaga tttaaataat
    10261 attgaagaag ccataagttg gtataagaaa gcatctgaaa atggagatac gaggtctagt
    10321 tatagattgg ggtgtatata tgagagctta ggaaatacaa aaaatgctag aaagtatttt
    10381 gaaatggcaa gtagcaaaaa tcatatgaat gctcgtatac atctaggtag aatttatttt
    10441 agagaaggta aattggaaga gtctaaaatg atgtttgata cacctgccaa tgaaaataat
    10501 gtgtacgctc aacatatggt aggattaatt tatgatatgt tctataaaga ttatgtaaat
    10561 tctaaatttt ggtatgaaaa ggctagagca caaggttgtg ttgaatcaat atataattta
    10621 ggccaaattt atttaaaatt aaatgatgac gcagaagctg agaaatatta taaagaaggt
    10681 attaaatatg gaagtaagaa gtgtgagtat atgcttgcag gtctttatta taaaaagagc
    10741 ctagatatgt acatttcttt agcaaatgaa gagtacgata attgtgaaga gatagtagaa
    10801 ggctttccca atttaaatat agactttgat gaagtattaa ttcctccatt taagttgcaa
    10861 gaagaggtaa tagatgaaga agagtatgta ccaatttata tactaaatat aaaagaaagt
    10921 ttagattcaa tgctagaagg gcttgaaact gaaatgatta tagatgaaga acatgatagt
    10981 taaaaagtat tataagatta tatagattta aatatataaa agccattagg ataaatgtaa
    11041 atcaattaat tctaatggct tttttattaa tattttataa tagcttattt cttgacatga
    11101 aaaatgtaat cactatacat attatagagg taataagaag tattatccaa aatccgtttg
    11161 ggttattaga aaatggcatg ttgatgacat tcataccaaa gaatcctgat attattgtag
    11221 gtattgagaa aattagtgta actactgtta aaatttgcat aacattattc tgattattac
    11281 ttataattgc actgaaagca tccataattc tactcaatat atctccatat attttagcca
    11341 tatcaagagc ctgtctattt tcaactataa catcttctaa taggtcttca tcatctggat
    11401 acttctttat accagcagtt ctaaccattt tgtttaaaac aagttcatta gattttagtg
    11461 aggtagagaa gtataccaaa gatttttcta attccaataa ttgaaccaat tctttgtttt
    11521 tcattgattt gtaaatttct cgctctatta tgattgtcat tttatttatt tttcttaagt
    11581 aatgcaagta ataagctgca tttttgtgaa gaatttgaag caaaaagcga gtctttttaa
    11641 acgtaaaaaa atctttaata tgaccaacta taaagtcatt taaaatctta gtctgtgcat
    11701 cacaaactgt tacaatagct tcttcagtta gtataatacc taagggtatt gtagtataat
    11761 gagaagaatt agagtcactt tcgtctactg gtatatctat aagtataaga gtatgattgt
    11821 cttcaacatc tattcttgag cgttcttctt catctagtgc agattctatg ctttctacat
    11881 ctatatttaa taaattagaa atttcgttga tttcactatg attaggttca acaagattta
    11941 tccaacagcc atcttcaaaa aaactaagtt tctctaattt tgaatctata gttttgtaat
    12001 atcttatcat ggcaacacat cctttattca gttttatata aatctcatca tttaatagta
    12061 tctattaatt aactaattta tataactgaa tatatgtatt agttttgatt ttagatatta
    12121 ttaagtgatg aaaatgtaaa aaattctctc cgcaacggac tagtatccat tgcccatcac
    12181 tctccttatt tattatttta tatatagcta tttatgaaat gcgtatttta agcactcttt
    12241 aatataatac cttttccagg tatataaatc aagttttgat ttgattaaat aatattttta
    12301 ccataattta caca
//
LOCUS       DAMJWP010000096        12283 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.100, whole genome shotgun sequence.
ACCESSION   DAMJWP010000096 DAMJWP010000000
VERSION     DAMJWP010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12283
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.100"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(1..267)
                     /locus_tag="PC700_002190"
     CDS             complement(1..267)
                     /locus_tag="PC700_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HDF2581497.1"
                     /translation="MKNFILIYFIVINSIAFFSMYIDKKRAIRNEWRIKEATLMSIAV
                     IGGSIGSMIGMYSFRHKTKHIKFTFGIPFILFLQFLLVYFYILK"
     gene            494..1195
                     /locus_tag="PC700_002191"
     CDS             494..1195
                     /locus_tag="PC700_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581498.1"
                     /translation="MKFIVEKEVFDKLENVCFGVVVAKGIDNTKEIERISNLLDISID
                     RVEDYFKDKKVKESEEIIPYREAFRSLGMNPNKFMSSIEAMTTRVAKNKKLPHINPIV
                     DLGNSISLKYLLPMGAHDMDFRNDDVYVRFSKKGDKFVPFGETDVELMEEGELIYSVG
                     DMVKTRRWIWRQGEEGKITNSSKNIFFPIDGFTDANLDKVMSAREELAKLLKEIFNCE
                     IKVGFVDKDNPEMEI"
     gene            1277..1885
                     /locus_tag="PC700_002192"
     CDS             1277..1885
                     /locus_tag="PC700_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF2581499.1"
                     /translation="MKKTIIKNVNPMIENKFIGLFEIEYKNKLDEDKVWMVASRKSSE
                     QLKNIYLKNEEDSVDAVVIVGLHKSSKKLILIRQFRVPINGYIYELPAGLVDEGESID
                     ISVERELREETGLTLLEIKKNKSIDKVYLSPGMSDESVAFVYCICDGDITDEFLEPDE
                     EIEALLISQEDAKEILQSNHKIDTKAFLILQMFVSLGAKLFE"
     gene            2209..2673
                     /locus_tag="PC700_002193"
     CDS             2209..2673
                     /locus_tag="PC700_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4624 family lipoprotein"
                     /protein_id="HDF2581500.1"
                     /translation="MKKLVIILISIISVVSLTACKKSNESKYNNLNNKESGQTTIEMD
                     LDKNYDTSDPFVNARLFCVSNDIDVLDTEISFKMDGDSGIVEIKDNKTDETLWSNTWH
                     GSVDNDTFAISLSNIQKEKEYAICFTGIKINHAVVKVTFESNLVKEKEKPSK"
     gene            3310..4326
                     /gene="gap"
                     /locus_tag="PC700_002194"
     CDS             3310..4326
                     /gene="gap"
                     /locus_tag="PC700_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDF2581501.1"
                     /translation="MKIKVGMNGFGRIGRAVLRIAQEELGDNIEIVAINARATTESLA
                     HLFTYDSCYGTFRGEVEAKDEDTLVVNSKEIKILRYSDPEELPWKELGVDIVIESTGL
                     FTQREKAEKHIKAGAKKVIITAPGKNEDITIVIGVNEKEYDNQKHNIISNASCTTNCL
                     APFAKVLDEKFGIVKGLMTTVHSYTNDQRILDKSHKDLRRARAAAESIIPTTTGAAKA
                     VSRVLPQLEGKLNGFSLRVPTPTVSLVDLVCELKENVTSEQVNSVLKDAAEGELKGVL
                     GYCDKPLVSIDYKGDSRSSIIDALSTMVIEDNMVKVVSWYDNEWGYSSRTVDLVKYVA
                     EKLK"
     gene            4721..5890
                     /locus_tag="PC700_002195"
     CDS             4721..5890
                     /locus_tag="PC700_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581502.1"
                     /translation="MEKLTDRRQVKAVSMETFIFLILLVVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLETVFLIMAMAVLAGALSALLSEFGVIALINKIFAVFMKPLYGLPGASIA
                     GAITTYLSDNPAIIPFAKDKTFTQYFKQYQVPALCNLGTAFGMGLILTTFMISQGTEY
                     VLPALIGNLGAIIGSVISVRIMLTFTKKFYKYNPEEDKATGTLEKKDEFREIREGNVF
                     QRALDAILEGGKMGVDMGMAIIPGVLVVCTLVMLLTFGPSTDPVTGQEVYTGAAYEGI
                     KLLPVIGDKLGFILEPLFGFTSPEAIAFPITSLGAVGAAMSLVPEFIKSGAITPNDIA
                     VFTAMGMCWSGYLSTHIGMMDALNARQLAGKAILSHTIGGLCAGAAAHFIFTLVG"
     gene            6271..10524
                     /locus_tag="PC700_002196"
     CDS             6271..10524
                     /locus_tag="PC700_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HDF2581503.1"
                     /translation="MDELFHIGIDVGSTTVKVVVLNNSNNIVHKEYRRHYSDVKKSVK
                     EVLNGIYEKLGDINTTIIITGSGGIGISKKLGVKFVQEVISSTKSIEYFHPETDVVIE
                     LGGEDAKITYLSGGIDQRMNGICAGGTGAFIDQMASLLKTDASGLNELAKGYNVIYPI
                     ASRCGVFAKTDIQPLINDGANQTDIAMSIFNAVVVQTVSVLSCGRKIEGNVAFLGGPL
                     YFLSELREAFKRVLDLKDENIIFPENAQLYIAIGAGLLSVDEDNIQLKSLIEKLDSIK
                     NIEDGEVNLLEPLFKDKNEYEEFSRRHEKEKISYVDINSIKSNCYLGIDAGSTTTKAA
                     LIDEDGRLVYSYYNSNEGNPLKTTIKVINEVYDILPKNIKILSSTVTGYGEGLIKKAL
                     KIDNGEIETIAHYKAAKFFNKDVDFILDIGGQDMKCLKIKDGVIDNIILNEACSSGCG
                     SFLETFASSLSMTIEEFAHEGIYSQNPVDLGSRCTVFMNSRVKQAQKEGASVGDISAG
                     LSYSVIKNALFKVIKIRDLNEIGNNIVVQGGTFYNDLVLRSFEKLIGKNVIRPNISGI
                     MGAFGSALIAKEKYESGYKTSLLSKEELNNIKLEASVTRCKGCSNHCLLTINKFSDNE
                     IFISGNRCEKGEAIYGDKKIGLEKKKPINLFKYKYNRIFRYKPLEKEEAKNGEIGIPR
                     VLNMYEDYPFWFTFFNAMGFRVVLSDRSSKQLYETGITSIASETVCYPGKLVHGHIEN
                     LIAKGIKNIFYPSVTNENKEDSNADNYYNCPVVISYSEVIKNNVENIRNKNINYINPF
                     ISLNDKEKLKKRLYDELSRHFSDIKITKEEVNHAVDKATDEQNLFKMEIQAAGERALK
                     EIKERNMKGIVLCGRPYHIDPEINHGMPELINSLDMAVLTEDSICHLANVQRPLRVVD
                     QWVYHSRLYKAASFVRDKNYLELVQLNSFGCGLDAVTTDQVQEILNEKSKIYTIIKID
                     EGNNLGAAKIRMRSLKAAMFERESKNIDVKNIKVNRIQYAKNNQITNKHTILAPQMSP
                     IHFQFLEKAVNLSGYNIEILKDTDSSVIEEGLRYVNNDACYPAIIVVGQLITALKSGK
                     YDLNNTSVTITQTGGGCRATNYIGFLRKAIYDAGFKDVPVIALSVNGIEDSGIMDNIS
                     LKLINRLFMSVVYGDLLMKVLYRVRPYEKVTGSANALYEKWVDICKSSLVKAKISVFT
                     RNIKDIVKEFDNLEILDIKKPKVGLVGEILVKFHPIANNNLVDILEREGAEAVVPDLT
                     NFFLSCAFNTIYKHTHLEGSRKSRMIGEAFIYITGIYQRVYKKALDKSERFYAPANIK
                     NVAKSTEPVVSLGNQTGEGWLLTGEMVELLNEGVENIICMQPFGCLPNHIIGKGSIKE
                     LKRLYKNANIIPIDYDPSASEVNQLNRIKLMLSKAFKNI"
     gene            10673..11167
                     /locus_tag="PC700_002197"
     CDS             10673..11167
                     /locus_tag="PC700_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDF2581504.1"
                     /translation="MSLVYDVYKSKLGNLYILSEDGFIVSVYIGDEKFNNLKNECIKK
                     GIDIKISKEDLKEAISQIDDYFNGKRKVFNLNIRIEGTEFQKSVYREMLKIPYGETLC
                     YSDIARNINNPKSVRAIGQASKSNKIPIIIPCHRVVGKKNIGGYMGNHSDLKEILLNL
                     ERQS"
     gene            11283..11660
                     /locus_tag="PC700_002198"
     CDS             11283..11660
                     /locus_tag="PC700_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HDF2581505.1"
                     /translation="MCDKVIASKYHEKGFNCAESVIKAYNEEFNTDIPVCLGSGLGSG
                     CGVASLCGAVNASNIIIGYVKGRNHEDESTKAKVYAKDLTTTVRKEYGSELCIDLKKD
                     LVACREIMDFAYDSLKETLKKEL"
     gene            11866..>12283
                     /locus_tag="PC700_002199"
     CDS             11866..>12283
                     /locus_tag="PC700_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufBD protein"
                     /protein_id="HDF2581506.1"
                     /translation="MKNLNLLINKLYSKNHNEAYKTFLFLENESLKSNITYCFFDSFL
                     EMIDNENSYIRTRGLLLISANAQWDIDNKIEINIDSILSHIVDKKPSVSRMFIKSIPN
                     ITKYKENLINRIKMELSNADISIYNNNMKPLVEKDIN"
BASE COUNT         5016 a         1214 c         2161 g         3892 t
ORIGIN      
        1 ttactttaaa atataaaaat atactaataa aaattgtaaa aatagtataa atggtatacc
       61 aaatgtaaat ttaatgtgtt tagttttatg tctaaaagaa tacattccaa tcatagaacc
      121 tatactacct ccaattactg ctatagacat aagagtagct tcttttattc tccattcgtt
      181 tctaattgct cttttcttat ctatgtacat tgaaaagaaa gcaatagaat taatcactat
      241 aaaatatatt agaataaaat ttttcacagt cttgcctcca taaaaatata ttatacatta
      301 taaaacaaga tatttgtttt ggaaagttaa atttgtaatt agtacatata aaatatctga
      361 taagaataaa tataatatta attggaaaat ttaaaaaaat taaacaatta gcaaataggg
      421 ggtatattta taagacacaa agtgataaaa tctatttaga aaataatgta aaataacagg
      481 aggataaagc aaaatgaaat ttatagtaga aaaagaagtt tttgataaat tagaaaatgt
      541 atgctttggt gtagtagttg caaaaggaat agataatact aaagaaatag aaagaataag
      601 caacctttta gatataagta tagatagagt agaagattac ttcaaagata aaaaagtaaa
      661 ggaatcagaa gaaattatac catatagaga ggcatttaga agtctaggca tgaacccaaa
      721 taaatttatg agttcgatag aagcaatgac aacaagggtt gctaagaata agaaacttcc
      781 tcatataaat cctattgtag atttaggaaa ctctatttct ttaaaatatt tacttccaat
      841 gggtgctcat gatatggatt ttagaaatga tgatgtatat gttagatttt ctaaaaaagg
      901 ggataaattt gtaccatttg gagaaactga tgttgaatta atggaagaag gagaattaat
      961 ttattcagtt ggagacatgg taaaaactag aagatggata tggagacaag gagaggaagg
     1021 aaaaattact aatagctcaa aaaatatatt ttttcctata gatggtttta ctgatgctaa
     1081 tttagataag gtaatgagtg caagagaaga attagcaaaa ttgttaaagg aaatatttaa
     1141 ttgtgaaatc aaagtaggtt ttgtagataa agataatcct gaaatggaaa tataataagt
     1201 tgttgaatac ttgttaagat attatattta cttaatgatg ttaaaaaggt attaaaaaag
     1261 aaaggtcgta gtaaatttga aaaaaactat aataaagaat gtaaatccaa tgattgaaaa
     1321 taagtttata ggactttttg aaatagagta taaaaacaaa ttagatgaag ataaggtttg
     1381 gatggtagca tcaagaaaaa gtagtgagca attaaaaaat atttatttga agaatgaaga
     1441 agatagtgta gatgcagttg taatagttgg gcttcataaa agtagtaaaa aattaatttt
     1501 aataagacaa tttagagtac ctattaatgg atatatatac gagttaccag caggtcttgt
     1561 tgatgaagga gagtctatag atatttctgt tgaaagagaa cttagagaag aaacaggact
     1621 tactctttta gaaattaaaa aaaataaaag tattgataaa gtttatttat ctcctggaat
     1681 gagtgatgaa tctgttgcct ttgtttattg tatatgtgat ggtgatataa cagatgaatt
     1741 tttagaacca gatgaagaga ttgaagcgct tttaatttcg caagaagatg caaaagagat
     1801 tttacaaagt aatcataaga tagacacaaa agcatttttg attttacaaa tgtttgtgag
     1861 tttaggagct aaattatttg aatagatata tagaaaagag gttatataga agagtaaaat
     1921 aataaagttt attttacttg tataaattat aatctctttt tttatacaat aaagtataat
     1981 tttgtgtatt tttaaataag ttacttgact cttaaatttt cataatatgt tataaaccaa
     2041 tttaagatac tacaataatt aaaattccaa aattatttag catatttgca attaaaatga
     2101 gaaaaggtaa aggctgaatg attgaaaaat ttgatgtaaa atagtagatt agtttaatat
     2161 tatagtttgt agggttaaaa attagaaatt gtaaaggaga taaattctat gaaaaaatta
     2221 gttattattt taattagtat cattagtgtg gttagcttga cagcttgtaa aaaaagcaat
     2281 gaaagtaaat ataataattt aaacaacaaa gaatcaggac aaacaaccat tgaaatggac
     2341 ttagataaaa attatgatac ttcagaccca tttgttaatg cacgtttgtt ttgtgtgtct
     2401 aatgatatag atgttttaga cacagagatt tcttttaaaa tggatggaga tagtggtatt
     2461 gtggaaataa aggacaataa aacagatgaa actttatgga gtaatacatg gcatggaagc
     2521 gttgataatg atacattcgc aatttctctt tctaatatac aaaaagagaa agagtatgct
     2581 atatgcttta ccggaattaa aataaatcat gcagttgtaa aggttacttt tgaaagcaat
     2641 ttagttaagg agaaagaaaa accctcaaaa taaattccag cttgtctata acttgatatt
     2701 cagtacataa aagcagttga tatttatgat tgactgcttt ttcctatcaa aaaggatagg
     2761 ttgtgcattt ttacttgaaa tagtttatgc acatcaaaag ttggtgagca tgatattgga
     2821 aatggtaagc atgaaatctt gataacacaa ggaaataaat ttaagttttc aattaagtat
     2881 atttccataa tttagttaac aataagctaa ttattattta tattttgata tataaataat
     2941 aattgtggca aattttaaaa aatcatatat tttttgaact aaataaaaat ttgtttttat
     3001 tattttaaat tagaggagat tcttgaagat atattatttt taaaaataag tatagtttaa
     3061 aaattcaaaa aaaagatggc aaaatatgtc ccaagggaca ttgagtaaaa taaatgattt
     3121 cagcttattt acactaaaaa gtacatacta aatgaggggt gaaataaaga aaaatgatat
     3181 actaattgtc ttttgataat aaaaaaacat ggaatttgat tatttaatca agaaaaagta
     3241 ttgaacaaat gatttattta gtgtacaata atcatgtaaa aacatagtaa tggagggaga
     3301 catttcataa tgaaaattaa ggtaggaatg aacggatttg gaagaatagg aagagctgta
     3361 ttaagaatag ctcaagaaga attaggagat aatatagaaa ttgtagccat aaatgcaaga
     3421 gcaacaacag aatctttggc acatttattt acatatgatt catgctatgg aacttttaga
     3481 ggtgaagtag aggcaaagga tgaagacact ctagtagtaa actctaaaga gataaaaata
     3541 ttaagatata gtgacccaga agaattacca tggaaagaac tgggggttga tatagtaata
     3601 gaatcaacag gattatttac acaaagagaa aaggcagaaa aacatattaa agcaggagca
     3661 aaaaaagtaa ttataacagc tccaggaaaa aatgaagata taactatagt tatcggagtt
     3721 aatgagaaag aatatgataa tcaaaagcac aatataattt caaatgcatc ttgtactaca
     3781 aattgtctag ctccttttgc taaagtattg gacgaaaaat ttggtatagt aaaagggtta
     3841 atgactacag tacattcata tactaacgat caaagaatat tagataaaag ccataaagat
     3901 ttgagaagag caagagcagc ggctgaatct ataataccta ccactacagg agcagcaaaa
     3961 gctgtgtcaa gagtactacc acaattagaa ggaaaattaa atggtttttc acttagagta
     4021 ccaacaccaa cagtttcact tgttgattta gtgtgcgaac ttaaagaaaa cgttacatct
     4081 gagcaagtta actctgtgtt aaaagatgct gctgaaggag aattaaaagg tgttttagga
     4141 tattgtgaca agccacttgt ttctatagat tataaaggag attcaagatc atctattata
     4201 gacgctctat ccacaatggt tatagaagac aatatggtta aggtagtttc ttggtatgat
     4261 aatgaatggg gatattcttc aagaactgtt gacttagtaa aatatgtagc tgaaaaatta
     4321 aaataatata tatttcttaa agcaaatttt aaaaaaattt tttgaaaaaa ttatataaaa
     4381 ctaaaaaaaa agattatgaa aaatattgaa ggaaaaattt tttcataatc ttttttcttt
     4441 catgcaaaaa gatacaagtt acaacttata tttgcatatt aggtcgttag agaagtatat
     4501 atatatttag taaggaatca tactttttat aaaatgaaag ataagatgtc gaaaacttgc
     4561 ataaatctga aaaattttta ttattaaaat actgattttc attttaaaaa tgataaaaaa
     4621 atgacaaata aagttgcaaa tagtaatact tataagtata attaaaaaag agaaaaggtt
     4681 ttgttaaact ttataacaaa aaaagaaggg atgattcaaa atggagaagt taacagacag
     4741 gagacaggtt aaggcagtaa gcatggaaac cttcattttc ctaatattac tagtagttgg
     4801 ttttgggtat gttggtagta ttatgggagc aggaatgatg ttcaaagtaa taatgagtac
     4861 agcacatgct ttactattgg agacagtttt cttaattatg gctatggcag ttcttgcagg
     4921 cgcattaagc gcactacttt cagagtttgg agtaatagcg ttaattaata aaattttcgc
     4981 tgtatttatg aagcctttat atggactacc tggtgctagt atagcaggag ctataacaac
     5041 ttatttatca gataatccag ctattatacc ttttgctaaa gataaaacat tcacacagta
     5101 ttttaaacaa taccaagttc cagcattatg taacttagga acagcctttg gtatgggact
     5161 tatcttaaca acatttatga tttctcaagg tacagaatat gtacttccag cattaattgg
     5221 taatttaggt gcaattatag gtagcgtcat aagtgttaga ataatgttga catttactaa
     5281 gaagttttat aaatacaatc cggaagaaga taaagcaaca ggaacattag agaaaaagga
     5341 tgaatttaga gaaatcagag aaggaaatgt atttcaaaga gctttagatg caattcttga
     5401 aggtggtaaa atgggggtag atatgggaat ggctataatt cctggagtcc tagtagtatg
     5461 tacattggtg atgttattaa cttttggacc atctacagac cctgttacag gtcaagaagt
     5521 ttatacagga gctgcatatg aaggaataaa attacttcca gttattggag ataaacttgg
     5581 atttatatta gaaccattat ttggatttac atcaccagag gcaatagcat tccctataac
     5641 ttcattggga gctgttggtg cagcaatgtc tctagttcca gagtttataa agtcaggagc
     5701 tataactcct aatgatatag cagtatttac agctatgggt atgtgttgga gtggatattt
     5761 aagtacacat attggtatga tggatgcatt aaatgctaga cagttggcag gtaaagctat
     5821 tctttctcat acaataggtg gtttatgtgc aggagcagca gctcacttta tatttacact
     5881 cgtaggataa acgtaatcag ctaagataaa aaataatata caaaaaaatg gttgttgata
     5941 tgtaataaga atttacatat caacaaccat ttttttctga ctaaagataa tttataatac
     6001 ataaacatta agtggatatt agacttaagt tatatttatg taaaaagtaa caaaaatctg
     6061 tagaaattta tgacaaaatt tagtacacta actatatgtt cttatttaaa atttaaggtt
     6121 agaattagga attacctagt agagtaagct ttgattaata aaagaatcat aaaataaaaa
     6181 tattagtcat aaagattata aataacttac ttaaaataaa gccatagaac ttacaaaaat
     6241 taaaaaagca tataaatatg gaggggtaca atggatgaat tatttcatat aggaattgat
     6301 gtaggttcta caacggtgaa ggttgtggta cttaataata gtaataatat agttcataaa
     6361 gaatatagaa gacattattc tgatgtaaaa aaatcagtta aagaagtact aaatggtata
     6421 tatgaaaaat tgggagatat aaatactacc attataataa ctggttcagg tggaattgga
     6481 atatcaaaaa aattaggtgt aaaatttgtt caggaagtta tatctagtac aaaatcaata
     6541 gagtattttc atcctgaaac tgatgttgtc atagaacttg ggggtgaaga tgcaaagata
     6601 acatatctaa gtggaggaat agaccaaaga atgaatggaa tatgtgcagg tggcactgga
     6661 gcattcatag accaaatggc atcactttta aagacagatg ccagtggatt aaatgaactt
     6721 gctaaagggt ataatgttat atatcctata gcttcaagat gtggtgtttt tgctaaaaca
     6781 gatattcaac ctcttataaa tgatggagcc aatcagactg atatagcaat gagtattttc
     6841 aatgcagttg tcgtacaaac tgtgagtgtc ctttcttgtg gaagaaaaat agaaggaaat
     6901 gttgcgtttt tgggtggtcc attgtacttt ttatctgaac ttagagaagc atttaaaaga
     6961 gtattggatt taaaagatga aaacataatc ttcccagaaa atgcacaact ttatattgct
     7021 attggagcag gattattatc agtagatgaa gataatatac aactaaaatc tctaatagaa
     7081 aaattggata gtataaagaa tattgaagat ggagaagtaa atttattaga acctttattt
     7141 aaagataaaa atgagtatga agaattttca agaagacatg aaaaagaaaa aataagttat
     7201 gtggacataa attcaataaa aagcaattgt tatttaggaa ttgatgcagg ctcaactaca
     7261 actaaagcag cacttattga tgaggatgga agacttgtat attcttatta taatagcaat
     7321 gaaggaaatc cattaaaaac aaccattaaa gttataaatg aagtatatga tatattacca
     7381 aaaaatatta aaatattgag ttcaactgta actggatatg gagaaggtct tattaaaaaa
     7441 gctcttaaaa tcgataacgg agagatagag actatagcac attacaaagc tgctaaattt
     7501 ttcaataaag atgtagattt tatacttgat attggtggtc aagatatgaa gtgcctaaaa
     7561 ataaaagatg gagtaattga taatataata ttaaatgagg cttgttcatc aggttgtggc
     7621 tcatttttag agacatttgc aagttcttta tctatgacaa tagaagaatt tgcacatgaa
     7681 ggtatttatt ctcaaaatcc tgtagattta ggttcaagat gtactgtatt tatgaattct
     7741 agagtaaaac aagctcaaaa agaaggtgca agtgttggag atatatcagc agggctatct
     7801 tattcagtaa taaaaaatgc tttatttaaa gtaataaaga ttagagattt aaatgagatt
     7861 ggtaataata ttgttgtaca aggtggaacc ttttataatg atttggtgct tagaagcttt
     7921 gaaaaactaa ttggaaaaaa tgtaattaga cctaatattt caggaattat gggagctttt
     7981 ggttcagcac ttatagctaa agaaaaatat gaaagtggat acaaaacatc cttgttatct
     8041 aaggaagagt tgaataatat caagctagaa gcaagtgtaa ctagatgtaa aggttgttca
     8101 aatcattgtc ttcttacaat aaataaattt tctgataatg aaatttttat ttcaggtaat
     8161 agatgtgaga agggtgaagc tatttatggt gataaaaaaa taggattaga aaagaaaaaa
     8221 ccaataaatt tatttaagta caaatacaat agaattttta gatacaaacc attggaaaaa
     8281 gaagaagcaa aaaatgggga aatagggata ccaagagtat taaatatgta tgaagattat
     8341 ccattttggt ttactttttt caatgctatg ggctttagag ttgtactttc agataggtca
     8401 tcaaaacagt tatatgaaac tggtataact agtattgcat ctgaaacagt ttgttatcca
     8461 ggaaaattag ttcatggaca tatagaaaat ttaattgcaa aaggtataaa aaatatattt
     8521 tatccaagtg taacaaatga aaataaagag gattcaaatg cagataatta ttataattgt
     8581 cctgttgtaa tatcatattc tgaagtaata aaaaacaatg tagagaatat aagaaataag
     8641 aacataaatt atataaatcc atttataagt ctaaatgata aggaaaaatt gaagaaaaga
     8701 ctatatgatg aattatcaag acatttttct gatataaaaa taactaaaga agaagttaat
     8761 catgcagttg ataaagctac tgatgaacag aatttattta aaatggagat acaagcagca
     8821 ggtgaaagag cacttaaaga aataaaagaa agaaatatga aggggattgt gttatgtgga
     8881 agaccatatc atatagaccc agaaataaat catggaatgc cagagcttat aaattcatta
     8941 gatatggcag tgcttactga agactctata tgtcatttag ccaatgtaca aagaccactt
     9001 agagttgtag accaatgggt atatcattca agattgtata aagcagctag ttttgtaaga
     9061 gataaaaact atttggaact tgttcaactt aattcatttg gctgtggatt agatgcagta
     9121 actacagacc aggttcaaga aatacttaat gaaaaatcga agatatatac tattataaag
     9181 attgacgaag gcaacaattt aggagcagct aagataagga tgaggtcctt aaaagcagct
     9241 atgtttgaga gagaaagcaa aaatatagat gtaaaaaata taaaggtaaa caggatacag
     9301 tatgctaaaa ataatcagat aacgaacaaa catactatat tagcaccaca aatgtctcca
     9361 atacatttcc aattccttga aaaagctgtt aacttaagtg gttataatat agaaattcta
     9421 aaagatactg atagtagtgt aattgaagaa gggttaagat atgtaaataa tgatgcatgt
     9481 tatccagcta taatagttgt aggtcagcta ataacagcat taaaatctgg caaatatgat
     9541 ttaaataata cttctgttac tataactcag actggtggag gttgtagagc tacaaattat
     9601 attggattct taagaaaggc tatatatgat gctggattta aagatgttcc tgtcattgca
     9661 cttagtgtaa atggaataga agatagtggt ataatggata atatatcact aaaattaatc
     9721 aatagactct ttatgtctgt tgtttatggt gatttgctga tgaaagtact atatagagta
     9781 agaccatatg aaaaagttac tggaagtgca aatgctctat atgaaaaatg ggttgatata
     9841 tgcaaatctt ctcttgttaa agcaaaaatt tctgtattta ctagaaatat aaaagatata
     9901 gtaaaagaat ttgataattt agagatacta gatatcaaaa aaccaaaagt aggtttggtt
     9961 ggagaaatat tggtgaaatt ccatccaatt gcaaataata atttagtaga tatattagag
    10021 cgagaaggtg ctgaagcagt cgtgccagac cttacaaatt tcttcttaag ttgtgccttt
    10081 aatactatat acaaacacac tcatttagaa ggaagtagaa agagtagaat gataggagaa
    10141 gcatttatat acataactgg aatatatcaa agagtatata aaaaagcact tgataagagt
    10201 gagagatttt atgctccagc caatataaaa aatgttgcta aaagtacaga accagtagtg
    10261 tcattaggta atcaaactgg agaaggttgg cttttaacag gtgagatggt tgaattatta
    10321 aatgaaggtg tagaaaacat tatttgtatg caaccttttg gatgtttacc taatcacata
    10381 attggtaagg gttctataaa agaattaaag aggttgtata aaaatgcaaa tataatacct
    10441 attgattatg acccttctgc aagtgaggta aatcaactta acaggataaa acttatgctt
    10501 tctaaggctt ttaaaaatat ataagattta tattttgata tgttaaaagt taatgaattt
    10561 aaaaatatac tctatattaa gtatctataa aaaagagtta tcctattttt tagggtaact
    10621 ctttattagt taaaaaatta tatgtatact gatattaaag gggggaagta atatgagttt
    10681 agtatatgat gtttataaat caaaattagg aaatttgtac atattaagtg aagatggttt
    10741 tatagttagt gtatacatag gagatgaaaa atttaataat ttaaaaaatg aatgtattaa
    10801 aaaaggaatt gatataaaga taagtaagga agatttaaag gaagcaataa gccagataga
    10861 tgattacttt aatggaaaaa gaaaagtctt taatttaaat attcgtattg aagggactga
    10921 atttcaaaaa tcagtttata gggaaatgtt aaaaatacct tatggagaaa cattgtgtta
    10981 ttcagatata gcaagaaata taaacaatcc aaaatcagta agagcaatag gacaagcaag
    11041 taaaagtaat aaaattccaa taataatacc ttgtcataga gtagttggca aaaaaaatat
    11101 tggtggatat atggggaatc attctgattt gaaagagata ctattaaatt tagaaagaca
    11161 gagttaactt agaaagaaat gtttttattg ttaatatttg atataataat agtaaaaaga
    11221 agtatttttt acaaaaacaa tgaagaattt attttaattt acaaaaatga gggggaattt
    11281 taatgtgtga taaagttata gcatcaaaat atcatgagaa gggatttaat tgtgcagaat
    11341 cagttataaa agcatataat gaggaattta atacagatat accagtttgt ttaggaagtg
    11401 gactgggttc agggtgtggg gttgctagtt tatgtggtgc agtaaatgca tcaaatataa
    11461 taattggata cgtaaaaggc agaaaccacg aagatgaaag tactaaagct aaagtatatg
    11521 ctaaagattt aactacaaca gtaagaaaag agtatggttc agagttatgt atagacctaa
    11581 aaaaagattt agttgcttgt agagaaataa tggatttcgc atatgatagt ctaaaggaaa
    11641 ctttaaaaaa agaattataa gaaaataagc tatcaagtat aaattatttg atagcttatt
    11701 tttatagtcg atactaaata aatcaaatat attttagaat tattatttgt aaataaacag
    11761 caaataattt taggattttt atttaatata gtttttattt atatttattt ttgaatatgt
    11821 tatactttgt ttaaataaat atttgtaatg agggagatga gaagattgaa gaatctcaat
    11881 ttacttatta ataaattata ttcaaaaaat cataatgaag catataaaac ttttttattc
    11941 ttagaaaatg aaagtttaaa atcaaatatt acatattgtt tttttgactc attcctagaa
    12001 atgattgata acgaaaactc atatattcga acaaggggat tgttattaat ctctgcaaat
    12061 gctcagtggg atattgataa taaaattgaa ataaatatag atagtatact aagtcatatc
    12121 gtagataaaa aaccttctgt atcaagaatg tttattaaat ccatacctaa tattacaaag
    12181 tataaagaaa atcttattaa tcgcataaaa atggaattaa gtaatgcaga tataagtatt
    12241 tataataata atatgaaacc attagtagaa aaggatataa acg
//
LOCUS       DAMJWP010000097        12167 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.101, whole genome shotgun sequence.
ACCESSION   DAMJWP010000097 DAMJWP010000000
VERSION     DAMJWP010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12167
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.101"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..1381
                     /locus_tag="PC700_002200"
     CDS             <1..1381
                     /locus_tag="PC700_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2581507.1"
                     /translation="GILACIILILQYSKKMVRLEKLNVDLKNIAYKDELTGVNTIEKF
                     KLDANQNICINKDKKFAVFYIDFENFKYINDIFGYDYGDMILKRYANLMMNDIGKYEI
                     FAREIADRFVALRCYVDKEDLVVRQRDVDNELINITNEIKNKHSITVVSGICCIEDVN
                     EKLSIDGLINRANFAQKTVKNKPGTNYAFYNDSIRKKMIEENTIKSRIHEAIEKREFI
                     VYLQPKVNLHSQKINCAEALVRWLTPDKGIISPAIFIPVLEKNFFIALVDKYVFEEVC
                     KWIRKRLDENKPFVQISVNVSRIQFYNTKFVETYSNIQNKYRIPKNTIEIEFTESVAF
                     ENQKHLLEIIHDLHENGFTCSLDDFGKGYSSLSVLKDLPFDVLKLDSMFFKASLDKDK
                     EKIVIKNIVHMLKELNITTVAEGIECKEQVEFLRDIGCDLVQGFVFYKPMPILEFEEI
                     LDKEFVYNS"
     gene            complement(1673..2272)
                     /locus_tag="PC700_002201"
     CDS             complement(1673..2272)
                     /locus_tag="PC700_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2581508.1"
                     /translation="MAIELKKGQKINLTKKENSDLGEILVNLNWNQKVQKKGFFGSLR
                     SSNIDLDLGCLFEMKNGVKGAVQALGNAFGSLDNPPFAQLDGDDRTGSNTQGENLRIN
                     GNKIKDIKRILIYAFIYEGVANWSEADGIVTIKQKQDSDLVVKLDEHKNGYNMCSIAL
                     IENVNDETFSVEKVVKYFKGHREMDTEFHWGLKWVAGRK"
     gene            complement(2375..3172)
                     /locus_tag="PC700_002202"
     CDS             complement(2375..3172)
                     /locus_tag="PC700_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2581509.1"
                     /translation="MKFKKLLCLLLCLVLTLAVVGCSKAKDDKKIVVGATLVPGGELL
                     EELKPLIKEKGYTLEVKNFDDYILPNEALNNGEIDANLFQHEPYLKEAVKAKGYKIMA
                     GKKLYVCPAILYSYKIKSVDEFKKGDTIAISNNPSSCSKNLRYLESIGLLTLPKGDGL
                     VSPKDIIENPKGIQFKELDIAQIPSSLPDVTAAFIDTTYAVPAGLDAKKNGIYTAPIN
                     DEYANLLAFRTEDKDSEKIKVLQDVLTSDKARSLIEEKYKGIVIPTF"
     regulatory      complement(3285..3390)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(3482..4468)
                     /locus_tag="PC700_002203"
     CDS             complement(3482..4468)
                     /locus_tag="PC700_002203"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDF2581510.1"
                     /translation="MLLIYNEKTNPYFNLAMEEYLLKNFDEDLFILWRNEPSIIVGKN
                     QNTLSEINLEYIKEYSIPVVRRQSGGGAVFHDLGNINFTFIACNNNNFSDFRRFTQPI
                     IDLLKTLDVNAEFSGRNDLLIDGKKFSGNAQYNYKNKVMHHGTLLFSSEINDLSKALK
                     VKPIKFEGKGIKSVKSRVTNISEHLHSKMDILEFKDAIMDYLSKTNTDNTNYCLNEHD
                     IKQIEKLTKSKYETWDWNFGNSPKYTLSNELKYSGGNVEFNLNVDKGIITSIKFFGDF
                     FGKCDVAFVENKLIGIRHEENALRNTLDSIEINDYFLGADTDILVSGILGVK"
     gene            4952..6682
                     /locus_tag="PC700_002204"
     CDS             4952..6682
                     /locus_tag="PC700_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HDF2581511.1"
                     /translation="MRNKKINIIFLTTIMFIMSTVMVFAEEDIDTIALANAEKFGILT
                     LIPPLVAIILAFITKNVIISLLIGILSGSFIIKASGINVFATFIQAFLDLVDRALVSL
                     ADPWNAGIILQVLAIGGVINLVAKMGGAKAIAEALAKRAKSAKGTQLITWFLGLLVFF
                     DDYANSLIVGPMMRPVADKMKISREKLAFIIDATAAPVAGLAIISTWIGLEVGLIHDA
                     FESISIDVDAFGIFLNTIPFRFYNILILAFIVISALLLKEFGPMRKAEIKSRSRKISI
                     DLDEGVEELDDLAPKNGVKLSVWNAIIPIGTLIIVALASFYYSGYTSIMGGDDKALIQ
                     LFTNSPYSFEAIKEAFSASDASRALFQSALVASLVAIIMAVVKKIFTISEAIDVWIDG
                     MKSLVITGVILILAWSLSSVIKELGTAKFLIHLLSGSLPPFLLPSLIFGLGAIISFAT
                     GTAYGTMGILMPLAIPLAYSLNPDMSYVIVSTSAVLTGAIFGDHCSPISDTTILSSMG
                     AGCNHIDHVNTQMPYAIFTAVITIVFGYIPAGLGLPIYIVLPVAIAAIFVGIQIIGKK
                     VDEAEIELVE"
     gene            6792..6872
                     /locus_tag="PC700_002205"
     tRNA            6792..6872
                     /locus_tag="PC700_002205"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6826..6828,aa:Leu,seq:tag)
     regulatory      7769..7873
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            8083..10557
                     /gene="gcvPA"
                     /locus_tag="PC700_002206"
     CDS             8083..10557
                     /gene="gcvPA"
                     /locus_tag="PC700_002206"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HDF2581512.1"
                     /translation="MNELKRVSLYNIHKELGAKLVEFAGWEMPLEYEGINKEHEKVRK
                     SAGIFDVSHMGEVQIKGAESEKFIQNLVTNDISTLKINDIIYTPMCYENGGVVDDLLI
                     YKFGEEDYLLVINAGNIDKDVAWIIKQSEGYNVDIKNISSEVSQLAIQGPKAEEILQK
                     ITDIDLNSIKFYKSIPSTKVCGCPCLVSRTGYTGEDGFEIYCKNKYVEIIWNEVLKVG
                     GEDICPAGLGCRDTLRFEAALPLYGHEINEHISPIEGGLSIFVKTNKESFIGKSILSK
                     EKESGAKRKLVGFEMQGKGMPRNGYDIRIGDKTVGFVTTGCASPTTGKILGMGIIDSE
                     YAKVGNEIGIAIRKKVVPAVIVKKPFYKKQYKKDNIILNKENKFSYIPATSEDKSKML
                     KVVGLNSVDELFSDIPEEVKLKRDLNLEIGKSELEVSKIVKRLSEENLSLEDLTCFLG
                     AGAYDHYIPSIIKHITSRSEFYTAYTPYQAEISQGTLQVVFEFQSMIAEITGMEIANA
                     SMYDGATAAIEACIMAMNQTRKSKIVVSKTIHHETLSVLRTYLQYKDCEIVEIDFCNE
                     YGTTDIEKLKASVDKDTACVLIQTPNFFGIIEEMEEIEKITHENKAMLIMSVDPISLG
                     VLKTPGEIGADIVVGEAQSLGNPLNFGGPYVGFLASKSKYTRKMPGRIVGQSLDVEGK
                     IAYVLTLQTREQHVRREKATSNICSNQALNALVASIYMATMGKEGFKEVGMQSMKKAH
                     YTYNKLVQTGKYKPIFKGKFFKEFAVQGNLNIETINDKLLEENILGGYNLEYNYPELK
                     NSTLLCVTEKRSKEEIDKLVGIMEGL"
     gene            10557..12014
                     /gene="gcvPB"
                     /locus_tag="PC700_002207"
     CDS             10557..12014
                     /gene="gcvPB"
                     /locus_tag="PC700_002207"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HDF2581513.1"
                     /translation="MKEYNSLLIDISKKGRKAYSLPKLDIDDIKIEDMIDQDMARQSE
                     LNLPEVGELELVRHYTLLSNKNFGVDTGFYPLGSCTMKYNPKINEDMAALPNFTGMHP
                     YQSSDTAQGSLSLMYDLSRRLAEITGMDEVTLQPSAGSHGEFTGLMIIKAYHENRGDH
                     KRTKVIIPDSAHGTNPASAAMANFDVIQIASDKNGAVDINALKEVLNDEVAALMLTNP
                     STLGLFEKNIKEIATLVHEAGGLLYYDGANMNAIMGITRPGDMGFDVVHLNLHKTFST
                     PHGGGGPGAGPVGVKKELVPFLPIPLIERKEDKYVLNYDREKSIGKIKNFYGNFGVLV
                     RAYTYILTMGRDGLKEASEMAVLNANYIKESIKDDYILPIDTLCKHEFVLGGLPRGEA
                     NIKTIDIAKRLLDYGYHPPTMYFPLIINEALMIEPTESESIETLDSFIEAMKSVAKEA
                     KEEPELLKTAPHNTLVKRVDDARAVKKPILTWSQR"
BASE COUNT         4541 a         1481 c         2020 g         4125 t
ORIGIN      
        1 aggtatatta gcatgtatca tattgatttt acaatatagt aaaaaaatgg ttagactgga
       61 aaaattaaat gtagatttaa aaaatattgc atataaagat gaactaacag gagtaaatac
      121 tatagaaaaa tttaaattgg atgcaaatca aaatatttgt ataaataagg ataagaaatt
      181 tgctgtattc tatattgatt ttgaaaactt taaatacatt aatgatatat ttggatatga
      241 ttatggtgac atgatactga aaagatatgc taatcttatg atgaatgaca tagggaaata
      301 tgaaatattt gcaagagaaa ttgcagatag atttgttgcg ttaagatgct atgtagataa
      361 ggaagattta gtagtaagac aacgagatgt agacaatgaa ctaattaata taacaaatga
      421 aattaaaaat aaacatagta ttactgttgt cagtggaatt tgttgcattg aagatgtaaa
      481 tgaaaaatta agtatagatg gtttaataaa tagagcaaat tttgcacaaa aaactgttaa
      541 gaataaacca ggtactaatt atgcttttta taatgacagt attcgtaaaa aaatgattga
      601 agaaaatact atcaagagta gaatacatga agctattgaa aaaagagaat ttatagttta
      661 tttacagcct aaagttaatt tacatagtca aaaaataaat tgtgcagaag ctttagttcg
      721 ttggcttact ccggataaag gtattatatc tccagcaata tttattccag tacttgagaa
      781 gaattttttt attgccttag tagataagta tgtttttgag gaggtttgta aatggattag
      841 aaagaggtta gatgaaaata aaccttttgt tcagatatca gttaatgttt caagaataca
      901 attttataac actaaatttg tagaaactta ttccaatata caaaataaat acaggatacc
      961 taaaaatact atagaaattg aatttacaga gagtgttgct tttgagaatc aaaaacatct
     1021 tttggaaatt atacatgatt tgcatgaaaa tggatttact tgttctttag atgattttgg
     1081 aaaaggttat tcgtctttaa gtgttttaaa agatttacca tttgatgttt taaagcttga
     1141 ctctatgttt ttcaaggcta gtttagataa agataaagag aaaatagtaa taaaaaatat
     1201 tgtacatatg ttaaaagagc ttaatatcac aactgttgct gagggcattg aatgcaagga
     1261 acaagttgaa tttcttaggg atattgggtg tgatttagtt cagggatttg tattttataa
     1321 accaatgcca atattagaat ttgaagaaat attggataaa gagtttgttt ataatagcta
     1381 aaatgtactt tataaaatat gaactaatta agtaaaatta ttttgtactg aataaatttt
     1441 tactttattt acagaatagt gattatttac agaaaagggc gaaaagtgta ttcctttaag
     1501 tatgggatgg atagcacaaa taggggagaa aaaatattgg ccatataaga atgcataata
     1561 atataaatat ttgaaaggtc agcattatat aaaaattaaa aagaaaataa ataatttttc
     1621 tataaaaaag agactatgag aaaagttact catagtctcc taaataatgt atttattttc
     1681 ttcctgcaac ccattttaag ccccaatgaa attcagtatc catttctcta tgacctttga
     1741 agtatttaac tactttttca acactgaatg tctcatcatt tacattttct atcaatgcta
     1801 ttgaacacat attatagcca tttttatgtt catcaagttt gacaactaag tctgaatctt
     1861 gtttttgttt gattgttaca attccatcag cttcagacca gttagctaca ccttcgtata
     1921 taaaagcata aataagtatt ctttttatat cttttatttt attaccattt attcttaaat
     1981 tttctccttg tgtattactt cctgttctgt catctccatc taattgagca aaaggtggat
     2041 tgtctaaact accaaaagca tttcctaatg cttgaacagc accttttaca ccatttttca
     2101 tctcaaatag gcaacccaaa tctaaatcta tattactgct tcttaaagag ccaaaaaaac
     2161 cctttttttg tactttttga ttccaattta aattaactaa gatttcacct aaatcgctat
     2221 tttccttttt tgtaaggttt attttttgtc ccttttttaa ttctatagcc atagtaatct
     2281 tcctccacaa acatagattt atttaaatgg tagcacttaa taagtcaata ttcaacaaga
     2341 ttagaataat aatattaaaa ctatactaaa tatactaaaa agtaggaatc acaatacctt
     2401 tgtatttttc ttcaattaag cttcttgctt tatctgatgt aagaacatct tgtagaactt
     2461 taatcttttc actgtcttta tcttcagttc taaatgctaa aagatttgca tactcgtcat
     2521 ttataggagc tgtataaatt ccgtttttct tggcatcaag accagcaggt acagcataag
     2581 tagtatctat aaatgcagca gtaacatctg gaagagaaga tggaatttga gcaatatcta
     2641 actctttgaa ttgtattccc tttggatttt ctattatgtc cttaggacta actaatccat
     2701 cccctttagg taacgttaaa agtccaatac tttcaaggta tctaaggttc ttagagcaag
     2761 aagatgggtt attgcttatt gcaattgtat ctcctttttt aaattcatca acagatttta
     2821 tcttataaga gtaaagtatt gctgggcata catatagttt tttaccagcc attattttgt
     2881 aacctttggc cttaacagct tcttttagat aaggttcatg ttgaaataga tttgcatcaa
     2941 tttcaccatt atttaaagct tcgtttggaa gtatgtaatc atcaaagttt ttaacttcta
     3001 aagtatatcc tttttcttta ataagtggtt ttaattcttc taataactct cctcctggaa
     3061 ctaaagtagc accaacaaca atttttttat catctttagc tttagaacat ccaaccacag
     3121 ctaaagttag tactagacat aataacaaac ataataattt tttgaatttc atataaattc
     3181 cctcctgaat ataattgatt agttaaaaat aaaataccat tctttatcac actgcaaaat
     3241 aaaaataagc ctgagtttaa actcaggctt taaaagctaa tttagactca tctttcagta
     3301 taatactgca ggatttagca ccaatgtata taaaaatata ctggttgctg ggtttcatag
     3361 ggccagtccc ttcaccactc tggataagaa gtatcaattt ttaattatgt tataaatatt
     3421 atcatgattt tttacgtctg taaacataat tttataaata ttttaaaatc tttaatgtat
     3481 tttatttaac acctaaaatt ccagaaacta gaatatcagt atcagcacct aagaaatagt
     3541 catttatttc aatagaatcc agagtatttc tcaaagcatt ttcttcatgc cttataccaa
     3601 taagtttatt ttctacaaat gcaacatcac attttccaaa aaaatctcca aaaaatttta
     3661 tactagttat tatgccttta tcaacattta aattaaactc aacatttcca ccagaatatt
     3721 ttaattcatt tgaaagagta tattttggtg aattaccaaa attccaatcc caagtttcgt
     3781 attttgattt agtaagcttt tctatttgtt ttatatcatg ctcattcaaa caataattag
     3841 tattatcagt atttgtttta gaaagataat ccattatagc atctttaaat tctaatatgt
     3901 ccatttttga atgtaaatgt tcacttatat tggttacacg agattttaca gattttattc
     3961 cttttccttc aaattttata ggtttaacct ttaatgcttt tgagaggtca tttatttcag
     4021 atgaaaataa aagagtacca tgatgcatta ctttgttttt gtagttgtat tgagcatttc
     4081 cagaaaattt cttgccatct ataagaaggt catttcttcc agaaaattcg gcattgacat
     4141 ctagtgtttt aagtaaatct attatgggct gagtgaatct tctgaaatca ctaaaattat
     4201 tattgttaca agctataaat gtaaaattta tatttcccaa atcatgaaat acagctccac
     4261 caccagactg tctcctaacg acaggtatag aatattcttt tatatattct aaatttattt
     4321 cagaaagtgt gttttggttt tttccaacaa ttatagaagg ttcattcctc cataaaataa
     4381 ataagtcttc atcaaaattt tttagaagat attcttccat tgctaaatta aaataagggt
     4441 ttgttttttc attataaatt aataacatta aagtagttcc tccatagaat tgataaaatt
     4501 tttattatga atacaattaa ttttataatt cgacaataat ataaaaactc ctttaaatta
     4561 gaaattttaa aaactacctt gcaaagttta aataaataat atatactcaa aatatgttta
     4621 ataaaagtga atagaagggt agaggcgcaa taatctatca gtactaataa ggagttaagc
     4681 acagagagtt attaggaaag gagatattgc cgaagtttta taatcgatgc tttaagatta
     4741 tattgctggg ggtacataga aaatatgtac aactgtcaca ggtttatttg tggtgagcta
     4801 tcatttaagg aattatatta tatttatttt tactattgtt gatatattgt aatgtttaag
     4861 ccttgagtgt ttaccactca aggctttttt agttttattg taacataata ccctgtacag
     4921 ttcacaaaat taaaaggaga gggagattta tatgaggaac aaaaaaatta atattatttt
     4981 tctgacgaca atcatgttca taatgtcaac agtaatggtt tttgcagaag aagatataga
     5041 cactattgca ctagctaatg cagaaaaatt tgggatttta actttaatac caccattagt
     5101 tgctataata cttgcattta taactaaaaa cgtaataatt tctttactta taggtattct
     5161 ttctggtagt tttataatta aagctagtgg tattaatgtt tttgcgacat ttatacaggc
     5221 atttttagat ttagtagata gagctttagt atctttagca gacccttgga atgcagggat
     5281 aatactacag gttttggcaa ttggaggagt aataaatctt gttgctaaaa tgggaggagc
     5341 taaagctata gctgaagcac ttgctaaaag agcaaagtca gcaaaaggga cacagcttat
     5401 aacatggttt ttggggcttt tagtattctt tgatgattat gccaattcac taatagtagg
     5461 tcctatgatg agacctgttg cagataaaat gaaaatatca agagaaaagc tagcatttat
     5521 aatagatgcc acagcagcac cagttgctgg tttagctata atatctacat ggataggtct
     5581 tgaggttggt ttaatacatg atgcttttga aagtataagt atagatgtag atgcatttgg
     5641 tatattttta aatacaatac catttagatt ttacaatata ttaatattag ctttcattgt
     5701 aatatcagca ttattattaa aagaatttgg tccaatgaga aaagctgaga taaaatctag
     5761 aagtagaaaa attagtatag accttgatga aggcgtagaa gagttggatg atttagctcc
     5821 aaaaaatgga gtaaaattga gtgtatggaa tgctataatc ccaataggaa cattgataat
     5881 agttgcatta gcatcatttt attatagtgg atatacatct ataatgggag gagacgataa
     5941 ggctctgata caactattta caaattctcc atattctttt gaagcaataa aagaagcatt
     6001 tagtgcatct gatgcatcta gagcattatt tcagtctgca cttgttgcaa gtttagttgc
     6061 tattataatg gctgtagtaa aaaagatttt tacaatatca gaagcaattg atgtttggat
     6121 agatggaatg aaaagtttag taatcactgg tgtaatatta atacttgcat ggtcattaag
     6181 ttcagtaata aaagagttag gtacagctaa gtttttaata cacctattat caggttcact
     6241 accaccattc ttattaccaa gtttaatatt tggtttagga gcaataatat cttttgctac
     6301 tggaacagct tatggaacaa tgggtatact tatgccactt gcaattccat tagcatactc
     6361 attaaatcct gatatgtctt atgtaatagt aagtactagt gcagttttaa caggtgcaat
     6421 atttggagac cattgttctc caatttcaga tacaacaata ctttcttcaa tgggagctgg
     6481 ctgtaatcat atagaccatg ttaacactca aatgccatat gctatattta cagcagtaat
     6541 cactattgta tttgggtata taccagctgg acttggattg ccaatatata tagtattacc
     6601 agtggcaata gctgctatat ttgtaggtat tcaaataatt ggtaagaaag ttgatgaagc
     6661 ggaaatagaa cttgtagaat aaagatataa ataagtttca ccttttatta tcactatttt
     6721 gttagttttt agataatttt aaaaagttat tgaaaataat ggcgaaatat agtaaaataa
     6781 aagagtgata agcggatgtg gcggaattgg cagacgcact agacttagga tctagcgcca
     6841 ttggcgtggg ggttcgactc ccttcatccg cactttacag tacttaattg tactgaaaaa
     6901 aactggggtt gctggctttc ggagtgggct atcccgtttt tatttttttg tatatataat
     6961 agattggcac tttagtataa ggtgcttttt ttataggcaa aaacagtaca tttatttctg
     7021 ttttattatc attcatctat tagaatatac tttatttaag aatgtgttaa taagcagtgt
     7081 tcacattctt gataaaatgt ggaataatat gcctaccaat aagcatttgg tgtataagta
     7141 agagtattta tatagttgat tgtgaaatga gtacaacaat tctgtacagt ataattggca
     7201 ttataattgt agcattgaat ttaaagtttg cacagtcgct gattggaaaa tttacaaagt
     7261 ggatgtcaaa catataaatt ttagtttatt ggacagttca aggaatcaga aattggaatt
     7321 ttatagtatt aatattttat agtattaata taatgtataa aattttatag caatgtattt
     7381 cataagtata aagtgactag aattttaaga aatatacaaa ttctataaaa actaaataat
     7441 ttcaagtata ttttaaaaat cttatttttc acaagtttaa aattgtaaac gatttcatca
     7501 agtctatgaa tatactttta acatgttata gtaaatctca atataaattt acatttggtt
     7561 ctttgtgtgt tattttaata ataaattaag tgaaaatgtt attttacatt aatatataat
     7621 atctttgcat aagagtgaat aatataaaag agaaagaatt gataataaaa acttaacaaa
     7681 aaaattaata cataaaaaaa aataaatata atgtgttgac atttaattga aatattcata
     7741 caataaaatt gaaaataaat atttccaaat gagattagcg agagagtcct ttatacgaaa
     7801 aggcgccgaa gaagaaattt aagaaatgcc aatttttaga gaagctttca ggcaaaaata
     7861 tcgctaattg atgggactct ggagagactt aagttaagat actataagtc accgaagaag
     7921 caaaataata ttaaattatt aaatctttca ggtatacaaa cagggatata taggctagga
     7981 ttattttcca tgctgatata tctttttttt atgaaaaagt acaggaaata atatcaaagt
     8041 tacttaaata cagtaggtct aattttctag ggagggttat atatgaatga attgaaaaga
     8101 gtatctttgt acaatataca caaagagctt ggagcaaaac ttgttgaatt tgctggctgg
     8161 gaaatgcctt tagaatatga gggtataaat aaagagcatg agaaggttag aaaaagtgca
     8221 ggtatatttg atgtatctca tatgggagag gtacagataa agggagcaga gtcagaaaaa
     8281 tttatacaaa acctagttac aaatgatata agcacattaa agataaatga cattatatat
     8341 acacctatgt gttatgaaaa tggtggagta gttgatgatt tattaatata taaatttggt
     8401 gaagaagatt atttattagt aataaatgca ggtaatatag ataaagatgt agcatggata
     8461 ataaaacaaa gtgaaggata caatgtagat ataaaaaata tatctagtga agtgagtcaa
     8521 ttagcaattc aaggacctaa agcagaagaa attcttcaaa aaatcacaga tatagactta
     8581 aactcaataa aattttataa gtcaattcca tcaacaaaag tatgtggttg tccttgttta
     8641 gtttcaagaa caggatatac tggggaagat gggtttgaga tatactgtaa aaataaatat
     8701 gtagaaataa tttggaatga agttctaaag gttggaggag aagatatatg tccagctggt
     8761 ctaggttgta gggatacttt gagatttgaa gcagctctac ctctatatgg tcatgaaata
     8821 aatgaacaca tatctccaat tgaaggtggt ttatctatat ttgtgaaaac aaataaagaa
     8881 tcatttattg gtaaatcaat tttatcaaaa gaaaaagaaa gtggagctaa aagaaagtta
     8941 gtaggatttg aaatgcaagg taaaggaatg cctagaaatg gatatgatat aaggattggt
     9001 gataaaacag ttggatttgt aacaacagga tgtgcatctc caacgacagg aaaaatttta
     9061 ggtatgggaa ttatagattc tgaatatgca aaagtaggaa atgaaatagg tatagcaatc
     9121 agaaaaaaag ttgttcctgc tgtgatagtt aagaaacctt tttataaaaa acaatataaa
     9181 aaagataata ttatattaaa caaagaaaat aaattttcat atatacctgc tacaagtgaa
     9241 gataaatcaa aaatgttaaa agtagttgga ttaaattcgg ttgatgaatt attttcagat
     9301 ataccagaag aagtaaaatt aaaaagagat ttaaatttgg aaattggaaa atctgaatta
     9361 gaggtaagca aaatagtaaa aagattgtca gaagaaaact taagtttaga agacttgact
     9421 tgttttcttg gtgctggtgc atatgaccat tatataccat caattataaa acatataact
     9481 tcaagatctg aattttatac agcatacact ccatatcaag ctgaaataag tcagggaact
     9541 ttacaagtag tttttgaatt tcaatctatg attgcagaaa ttacaggtat ggaaattgca
     9601 aatgcatcaa tgtatgatgg agcaactgct gcaatagaag catgtataat ggcaatgaat
     9661 cagactagaa aaagtaaaat agtagtgtca aaaactattc atcatgaaac tttgtcagta
     9721 ttaagaactt acttacaata taaagattgt gagatagtag aaattgactt ttgtaatgaa
     9781 tatgggacta cagatataga aaaattaaaa gcttctgtag acaaggatac agcatgtgtt
     9841 cttatacaaa ctcctaattt ctttggaatt attgaagaaa tggaagaaat agaaaagata
     9901 actcatgaaa ataaggctat gttaattatg agtgtagacc caatatcatt aggagtttta
     9961 aaaacaccag gtgagatagg tgcagatatt gttgttggag aagctcaatc acttggaaac
    10021 cctcttaatt ttggaggtcc ttatgtagga tttttagcta gcaaatctaa atatactaga
    10081 aaaatgccag gaagaatagt tgggcaaagt ttagatgtag aaggtaaaat tgcatatgta
    10141 ttaactttac aaactagaga acagcatgta agaagagaaa aagcaacttc taatatatgt
    10201 tcaaatcaag ctttaaatgc tcttgtagct tcaatatata tggctactat gggtaaagaa
    10261 ggatttaaag aggttggtat gcagtctatg aaaaaagcac attatactta caataagctt
    10321 gtacaaactg gaaaatataa gccaatattt aaaggtaagt ttttcaaaga atttgctgta
    10381 caaggaaatc ttaatataga aactataaat gataagctct tagaagaaaa tatattaggt
    10441 ggttataatt tagaatacaa ttatccagaa ttaaaaaatt caactttgct ttgtgtgact
    10501 gagaaaagaa gcaaagagga aatagataag ttagttggaa taatggaggg attataatga
    10561 aagaatacaa tagtttgttg atagatatat ctaaaaaagg taggaaagca tactcacttc
    10621 caaagttaga tatagatgat ataaaaatag aagatatgat tgaccaagat atggcaagac
    10681 aaagtgagtt gaatttgcca gaagttggtg aattagaatt agttagacat tatacattat
    10741 tatcaaataa aaactttgga gttgatacag gtttttatcc tttgggttct tgtactatga
    10801 aatataatcc taaaataaat gaagatatgg cagcacttcc aaactttact ggaatgcatc
    10861 cataccaatc ttcggataca gctcaaggtt ctctttcttt gatgtatgat ttatcaagaa
    10921 gacttgcaga aattacaggt atggatgaag ttacactaca gccttcagca ggttctcatg
    10981 gtgagtttac agggcttatg attataaaag catatcatga aaatagaggt gaccataaga
    11041 gaactaaagt gataattcca gattctgcac atggaacaaa tccagcaagt gcagctatgg
    11101 caaactttga tgtgattcaa atagcatctg ataaaaatgg agctgtagac ataaatgcat
    11161 taaaagaggt tttgaatgat gaagtagcag ctcttatgct tactaatcca agtactcttg
    11221 gtttatttga aaaaaatata aaagaaatag ctactcttgt acatgaagca ggaggacttt
    11281 tgtactatga tggtgcaaac atgaatgcta ttatggggat aacaagacct ggagatatgg
    11341 ggtttgatgt ggttcatctt aatcttcata agactttttc aactcctcat ggaggtggag
    11401 gtccaggagc tggtcctgtt ggagttaaaa aagaattagt acctttttta ccaataccat
    11461 taattgaaag aaaagaagat aagtatgtac taaattatga tagagaaaaa tctattggga
    11521 aaattaaaaa tttctatggt aattttggag ttcttgtaag agcttacaca tatatactaa
    11581 ctatgggaag ggatggactt aaagaggcta gtgaaatggc agttttaaat gctaattata
    11641 taaaggaaag tataaaagat gattatattt tgccaataga taccttgtgt aagcatgagt
    11701 ttgtattagg tggattgcca agaggagagg ccaatataaa aactatcgat attgcaaaaa
    11761 gactactaga ttatggatac catcctccaa caatgtactt tcctcttatt ataaatgagg
    11821 cacttatgat agaacctaca gaaagtgaga gtatagagac attagatagt tttatagaag
    11881 ctatgaaaag tgttgctaaa gaggcaaagg aagaaccaga actattgaaa actgctccac
    11941 ataatacttt ggtcaaaaga gtagatgatg caagggcagt aaagaaacct atactaacat
    12001 ggtcacaaag ataaataatt aaaaagtaga gatttaaagt ataaagagaa ttaaatttgt
    12061 aagaaaaagc tatctaactt aatccagttt agatagcttt tttgtatatt agtatgtatg
    12121 gtattttcta tataaagttt taaattatat tagtgcttat atagaat
//
LOCUS       DAMJWP010000098        12166 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.102, whole genome shotgun sequence.
ACCESSION   DAMJWP010000098 DAMJWP010000000
VERSION     DAMJWP010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12166
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.102"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            127..1170
                     /locus_tag="PC700_002208"
     CDS             127..1170
                     /locus_tag="PC700_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDF2581514.1"
                     /translation="MKRKIAVVLLTVAMLLSLVACSNDNTSKSDKLEDTLVVYTTHGE
                     DMLGAISEEFTKETGVNVEFINLKGELADRVRSEKDNPQADIMFGGDTSTYMQLKEEG
                     LYEKTEPSWNNELDKSYKDSDGYWYGTIKTPVMMFYNTKLLSADKAPKDWSDLTKEEY
                     KDLIVTRDSLSSSMRSTISNLINYYSNTKSEEEAWKYLEDLNKNIKNYYNSGSVMYSA
                     IGKGEATISVAVLSDIIENKEKNNMPLEVVDAESGSVVITDCVAALKNAPHPNAAAKF
                     VDFVGSAKVQAMVANDFNRIPTLDSALKDSPKWMSKEYKAMDVDWSVIGKNQSNWIEK
                     WETDIIDNNKTIK"
     gene            1431..2305
                     /locus_tag="PC700_002209"
                     /pseudo
     CDS             1431..2305
                     /locus_tag="PC700_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421888.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2324..3316
                     /locus_tag="PC700_002210"
     CDS             2324..3316
                     /locus_tag="PC700_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2581515.1"
                     /translation="MKNIELKEINHYYGKEKVLHDINISIKEGEFFTLLGPSGCGKTT
                     ILRIIGGFIKPSSGSIYVGDKNITNLEPENRNMGTVFQNYALFPNMTVEENVSYGLKI
                     KKLSKKIIKEKCDNYLELSGMKDFRKKKIDELSGGQQQRVAVARSLATEPTMLLLDEP
                     MSNLDIALRIKMREEIREIQQKTGITTLFITHDQQEALAISDRIAVMDKGKVLQIGVP
                     IEVYKSPLNDFVANFVGTSNCIGKEDYVNFNIEKETKPYIYKRPEEMILLQNTNQSGF
                     VKVKVESKNFLGAILEYTVSNDGKKYQVTELNRLNNSNKLNVGDMAYLGVLQGD"
     gene            3318..5012
                     /locus_tag="PC700_002211"
     CDS             3318..5012
                     /locus_tag="PC700_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDF2581516.1"
                     /translation="MIKKINRSKFTSKNVIFIVLYSILFWILIGYIIIPIGNTFVQAF
                     QGKGGYSFEVFKEYFSNTNNLRVVSNTIILGLGSVFVCGIMGISLALYMTFVCDKHKK
                     IIHILLLSPMMIPGVIMVIACIQIYGESGIVTKSIEILLPFIKKPYDFSGLKGILFVV
                     AYTQYVYFYLNVYVALKYVDYSTVEAAKGMGASKLRVFFDVIWPVITPAVITSTIITF
                     ASGISSFSAPNLIGGGFKVLSTQIVRSKANNHMDIASVQVITLLLMGILFMILLQYYG
                     KKYGVVPSLKSQSYKVHNRKKDLFTFVCKVIIEIQIIMIMLPIVTIVYLSFVSTHSIM
                     TTIFPRDFTLENYQMIFQKSRVLQPILNSFKMSLMAVAVGLIITVPVSYLVIKKDTAY
                     NRLAKFIIMLPWSMPASAIAVNLINTFNQKSIFAFNESLIGGFYIVPIAYTIYALPLL
                     FSSNEVAMKSINLGLEESSRSLGAGRIKTFYNVIVPNMMPGIISGAILVFIRTIGEYT
                     MSALLYGVYNRPISISIVTNMQEFKIGVSMAYGVLVIGICYIALGIIFKLDRKRFT"
     gene            5563..6441
                     /locus_tag="PC700_002212"
     CDS             5563..6441
                     /locus_tag="PC700_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HDF2581517.1"
                     /translation="MLINMKEMLKVAQENQFAVPAFNIGSGQILKAVVQGANEKNAPV
                     ILAIHPNELSFLGDSFVVSCIEEANKSKVPMVIHLDHGENKEQILRAIRCGFTSVMID
                     GSHLPYEENVAISREIVEIAKGLNVSVEGELGTIGTTGTSSEGGTDEIIYTDSKLAKD
                     FVEKTGVDTLAIAIGTAHGIYPKGFKPELKLDLLKEIREVVDIPLVLHGGSSNPDEEI
                     AQAVKLGVCKINISSDVKSAYYKKCRELLEQNPSLYEPDTIYPPCIKAAREVIEFKMN
                     LFNAIDKLKCFYKNKN"
     gene            6477..8372
                     /locus_tag="PC700_002213"
     CDS             6477..8372
                     /locus_tag="PC700_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2581518.1"
                     /translation="MIGKILRSDCIVLDKQLQTKKDVINYMANHLYHGNYINNKEKFI
                     ADINKREKESSTNMGNLIAIPHAKSNVVNQPVMMYLRTDREITDNDGQKLRSFFMIAV
                     PESGSDEHLKIISKLATYLMEENFLKHLLSAETPYEILSIFNNKAAESGDFSNNQNKG
                     LIVGITGCPTGIAHTFMAKKALEEAGQELGYEVKIETHGAIGVENKLTKEDIKRAEYV
                     IIACDKEVQLSRFIGKKVLQKKVVDAKNVSLAKNLIINAENGMAKEIKPGGKTLEDSK
                     EMLQGSETKGVYKHLMSGVSEMIPFVVIGGIGIAIAFMFGIFASDPTHETYNPIAGFF
                     GQLGGDSAFKLFIPILAGFIAKSIAGRQGFPPAMIAGFMAVIGGSGFIGGMVAGFATG
                     YIALGVMKITENLPDVLKGVNAVLICPLLIAFTSGAIMFFVVNTPASWLNEALQSWLQ
                     SMSGTNKVLLGAILAGMMASDMGGPINKTASAFGLAMFSSNIFEPSAALMVGGMVPPL
                     AISLAMLIFKNKFTKEERKSSLPTAIMGASFITEGAIPFAAADPLRMIPANIIGSCIG
                     GAICMSLDITLMAPHGGIFVIPFACSAPVAYIACILIGTVITALIIGVAKPTIRSENS
                     EVELLGA"
     gene            8640..10547
                     /locus_tag="PC700_002214"
     CDS             8640..10547
                     /locus_tag="PC700_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2581519.1"
                     /translation="MLVSRQIEFLKLILNENKYRPIKYFKDKLNVSDKTLQKDLKMIE
                     KYLETFNIKIDIKRGYGILIEDLAKKNIDLINSLNIQSKENKSLSVEQRRMEILKYLL
                     LNSNNITSINQLADKYYVSKTSIVNDFKYIEAWIKEYNLKLNKTLEGTKIIGKEVDIR
                     KSIAKMMDDLLDENYEQHDISELTRLDSATFSALINLFDIDSIIFVETIITELEYNLG
                     YTINQPYYINLITHILICLKRIEEGNQIESQEEREISVDNLDELVYKNVTTLIDKIEN
                     RYRVKITKDEIMYIYIFLVSSGFSSENNSSYNDESDILSESEKVSSIMIENMSQFLNI
                     NLKNDDLLQKSLASHVRPMLNRLRYDIQIKNPLLGEIQERFSDVLGLCMMTINIMTEY
                     DNLKNISIDEVSYLATYFQAAIERNMSSKRVIVVCHTGYGTSQLLAARLKREFPEWTI
                     VDIVPMHQLSKRNLDDVDFVISTVNLDLKDKLHIVVSVLLMESDINNIKNALLSKPKK
                     SMNLDTNLLGMYLEDNIYFNFDVAQMESLIGVNLGSKYESISLGKDLNIYIHKQSKLN
                     KGIMNINNVTRTIDIYLEISNQSFLKNILMEISNLYRQKNYINYIIDCKSKEDIKILF
                     KNNLLEKTNGN"
     gene            10537..10986
                     /locus_tag="PC700_002215"
     CDS             10537..10986
                     /locus_tag="PC700_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2581520.1"
                     /translation="MEIREILTEESIILDLKSTTKEGIIQELVDKLYDTGSITLKSKF
                     IKALKHREKIGSTSIGYQMAMPHGKTKYVKKASIVIGKSKKGVDWPSIDGLPVKVIIM
                     FAMPQSGGNEDMILISKIAYILGGKNISERLLNVHTKKEILDIFYEV"
     gene            11086..11973
                     /locus_tag="PC700_002216"
     CDS             11086..11973
                     /locus_tag="PC700_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2581521.1"
                     /translation="MAIYLEMPKLIHEFPFRSLSNEGEVLTTPHWHKEIEILLITEGV
                     VNLFINDKPMQLKKGEIVIIKGGDIHYILPSPGSERLVFQFDISFFNDLILLNEEKES
                     LINLFSSIKKCSRDWSAKIRKSVFDILIDIYNEDLYKIEGYSYAIKGKMYNLIPILYR
                     QIPREIEENTVEYEINSKEILERLNNIFKYVEKNYKQPIKLEDVSYEVGFSVYYFTKF
                     FKKNTGKTFITFLNEYRLEKAKWILLNNNISIVDLVEEVGFGSTKTFYRVFKEKIGVS
                     PLEFKKRYNSLEKKGGASK"
BASE COUNT         4893 a         1274 c         2144 g         3855 t
ORIGIN      
        1 tagaaaaaaa caataataaa ataagctgtt atagaaaaat acaattatat agattgttgt
       61 tgaatcattg actgcaagaa taataacatt taaaatgaac atggagaata tgggggagga
      121 atcaatatga agagaaaaat agcagttgtt ttattaactg tagcaatgtt attatcactt
      181 gtagcttgta gcaatgataa cacatcaaaa agtgataaat tagaggatac attagtagta
      241 tatactacac atggagagga tatgctagga gcaatttctg aagaatttac aaaggaaaca
      301 ggagttaatg ttgaatttat aaacttaaaa ggtgaactag cagaccgtgt tagaagtgaa
      361 aaagataatc cgcaagcaga tataatgttt ggtggagata catctacata catgcaatta
      421 aaagaagaag gattatatga aaagactgaa ccaagctgga ataatgaatt agacaagtct
      481 tacaaagatt cagatggata ttggtatgga actataaaaa caccagtaat gatgttttat
      541 aacacaaaac tattatcagc tgataaagcg ccaaaagatt ggagtgattt aacaaaagaa
      601 gaatacaaag acttaatagt tactagagat tcattatcat catctatgcg ttctactatt
      661 agtaacttaa tcaattacta ctcaaatact aagagtgaag aagaagcttg gaagtatctt
      721 gaggatttaa ataagaatat aaagaattac tacaatagtg gaagtgtcat gtattcagca
      781 ataggtaaag gtgaagctac aatatctgtt gcagtattaa gtgatataat agagaataaa
      841 gaaaaaaata atatgccatt agaagttgta gatgcagaga gcggttctgt agtaatcaca
      901 gactgtgtag cagctctaaa aaatgcacca catccaaatg cagcagctaa atttgtagat
      961 tttgttggaa gtgctaaggt tcaagctatg gttgcaaatg attttaatcg tattccaaca
     1021 ttagattctg cacttaaaga ttcaccaaag tggatgtcaa aagaatataa agctatggat
     1081 gtagattggt ctgtaattgg aaaaaatcag tctaactgga ttgaaaagtg ggaaacagat
     1141 ataatagata acaataaaac tataaaatag cctaattaaa taatttaata ttatatacaa
     1201 aaatagtgag gattttcctc gctattttac gtataaggaa actatagtat ctcataattt
     1261 tgtataattc atacaacaaa atgatactaa tacatactta ttaaataaaa tagtttttga
     1321 gtaactgatg aagtaattga ctatataaag tgatttgtag acatagtctc tcaaataaag
     1381 tgaatttttt attatgcaaa acttttaatt atagtaaaag tacaatttat atgggcaagg
     1441 agataaaaat ggaactaaaa attagtgatt ataaattatt atgtaataaa ggtagtggcg
     1501 cttttaacga agatatcgta ggtttatcac cttatggggc atgggtactt gatggagcta
     1561 caggtttaaa taataaaaat ttagtctcta cagaaagtga tgccaaatgg tatgtaagtt
     1621 ggtggaatag ttatcttcat gagaatatgg aaagagaaga tagcttaaaa gtaattatta
     1681 aagaaggcat tgaggagata acaaaagagt actacaaatt aacagatgat aaagaagtag
     1741 aagcaataga tattccttct tgttcatgtt ctatattaaa gttttataaa gataagatag
     1801 agtatttact tttaggagat tgtactttat tagttaacaa taatgaatct gtgaaaattt
     1861 taaaagatga gactttatgt gaacttgata aaaaagtatt tgattttatg aaagaagcca
     1921 aagatgctac aaaccttact ttaaaggaga gaaaagccga ggtaatgcga ttataataga
     1981 gaatagactt aagaaaaata ctgtgggggg atattggact ttggaatttt ctaaagaggc
     2041 cgttgacaat tgtgtaagtg gatttattga tatagaatat aaaatgaaaa ttatgatggc
     2101 tagtgatggt ttttcatgtg gatatgatag atataagatt tttgataaaa atgaaatgat
     2161 gaaaatagca gagattaaag gtatagatta tatatataga aaggttaggg aactagaaaa
     2221 ggatgatgaa atggcaatag agtttcctag atttaaaatc tatgatgatt catcatgtgt
     2281 atatttagag atttataaat attagataat ttatggagac gatatgaaga atatagaatt
     2341 aaaagaaatt aatcattatt atggaaaaga aaaagtatta catgatatta atatatctat
     2401 taaggaagga gagtttttta ctttactagg tccttctgga tgtggaaaaa cgactatttt
     2461 aagaataatt ggtggattta taaaaccttc ttctggaagt atttatgttg gtgataaaaa
     2521 cattaccaat ttagaaccag aaaatagaaa tatgggaact gtttttcaaa actatgcact
     2581 gtttccaaat atgacagttg aagaaaatgt atcttatgga ttgaaaataa aaaagctgtc
     2641 taaaaaaatt ataaaggaaa aatgtgataa ttatttagag ctgtcaggaa tgaaagattt
     2701 taggaaaaaa aagattgatg aattatctgg tggacagcaa caaagggtgg cagtagcaag
     2761 gtcattagcg actgagccta ctatgctttt attagatgag ccaatgtcta atctagatat
     2821 agctttaaga atcaagatga gagaagaaat aagagagata cagcaaaaga caggtattac
     2881 tactttattt attacccatg atcaacaaga agccttagct atttctgata gaatcgctgt
     2941 aatggataag ggtaaagttc ttcaaattgg tgtgccaata gaagtttaca aaagcccatt
     3001 gaatgatttt gtagcaaact ttgtaggtac ttcaaactgt attggaaaag aagattatgt
     3061 taattttaat attgaaaaag agacaaagcc ttatatttat aaaagacctg aagaaatgat
     3121 attattgcaa aatacaaatc aaagtggttt tgtaaaagta aaagtagaat ctaagaattt
     3181 tctaggagca attttagaat atacagtatc aaatgatggg aaaaaatatc aagtaactga
     3241 gcttaataga ctgaataata gcaataaatt aaacgtaggg gatatggcat atttaggtgt
     3301 gctacaaggt gactaatatg attaaaaaaa taaaccgtag taaatttaca tctaaaaatg
     3361 ttatttttat tgttttatac agtatattat tttggatttt gattggatat attatcatac
     3421 caataggcaa tacttttgta caagcattcc aaggtaaagg tggatattct tttgaagttt
     3481 ttaaagaata tttttcaaat actaataatt taagggtagt aagcaatact ataattttgg
     3541 gattgggttc tgtgttcgta tgtggaatta tgggaatatc acttgcctta tatatgacat
     3601 ttgtttgtga taaacataag aaaatcatac atattctttt actaagtcca atgatgattc
     3661 ctggagtaat tatggtaatt gcatgtattc agatttatgg agaaagtgga atagttacaa
     3721 aatctataga aattttactc ccctttatca aaaaacctta tgattttagt ggattaaagg
     3781 gaattttatt tgtggttgct tatactcagt atgtatattt ttatttaaat gtatatgttg
     3841 ccttaaaata tgtggattac tcaacagttg aagctgcaaa aggaatggga gcatcaaagc
     3901 taagagtatt ttttgatgta atatggcctg ttattacacc tgctgttata acttctacta
     3961 ttataacttt tgcatcaggg attagttctt tctcagctcc aaatcttata ggtggtggtt
     4021 ttaaagtttt aagcactcaa attgttaggt ctaaggctaa taaccatatg gatatagctt
     4081 cagttcaagt aattacttta ttactaatgg ggattttatt tatgatttta ttacagtatt
     4141 atggcaaaaa atatggagta gtgccatcct taaaatctca aagttataaa gttcataata
     4201 gaaaaaaaga cttatttaca tttgtatgta aggttattat tgaaatacaa attattatga
     4261 ttatgcttcc tatagttact attgtgtatt tatcctttgt aagcacacat tcaattatga
     4321 caaccatatt tcctagagac tttacactgg aaaactatca gatgattttc caaaaatcac
     4381 gtgtattaca gcctatatta aatagtttca aaatgtcttt aatggcagta gcagtaggat
     4441 tgataattac tgtaccagta tcatatttag taattaaaaa ggatactgca tataatcgac
     4501 tagcaaaatt tattataatg ttaccttgga gtatgcctgc aagcgccatt gcagttaacc
     4561 taattaatac atttaatcaa aaaagtatat ttgcttttaa tgaatcctta ataggaggtt
     4621 tttatattgt acctatagca tatacaattt atgctctacc attattattt tcatccaatg
     4681 aggttgctat gaagtcaata aatttaggac tagaagaatc atcaaggagt ttaggtgctg
     4741 gaaggataaa aaccttttat aatgtaatag ttccaaatat gatgccagga ataatttcag
     4801 gagctatatt agtattcatt cgtacaattg gggaatatac aatgtcagcc ttgctttatg
     4861 gtgtttataa tagacctatt tcaatttcta ttgtaactaa tatgcaagaa tttaagatag
     4921 gagtatctat ggcttatggt gtattagtaa ttggtatatg ctatatagca ttaggaataa
     4981 tttttaaatt agatagaaaa agatttacat aagataacaa aggctaattt ataaattggt
     5041 gcttagaaaa ccaaatcact ttattagcta aagatgaatt tgaagggtga cactttggtg
     5101 ttttatgtaa agtttatgga agtaaaacat taaataatcg tgagtatgat tcctataaat
     5161 cgtatatact attgaaatcc aagaaaaggt attggctaaa tagctaatat ctttttaatt
     5221 ttttgactcc catatagcat taactattac atgagtatct ttaaatttat agttaaagta
     5281 tcttaattgt ttattataaa aatataggat ggctaaagca cattaaatta aaacacttaa
     5341 aaagagaaga tgtacatgag aaatataatt aactatgata cagacttcta aaaattattt
     5401 agtgaaaagg aacttagagt gtataataaa agttcctttt caataaggaa aacgattgtt
     5461 attttctgag cttcattacc ctgatattat ttaaatataa acaacaacaa aattaacaag
     5521 aagtaaattc aacctaaaat ttaaaatatc gggaggtaca atatgttaat caatatgaag
     5581 gaaatgctaa aagtggcaca agaaaatcaa tttgctgtac cagcatttaa tataggaagt
     5641 gggcaaatac ttaaagcagt agtacagggt gctaacgaaa aaaatgcacc agtaatactt
     5701 gcaatacacc caaatgagct aagttttcta ggcgatagct ttgtagttag ttgcatagaa
     5761 gaagcaaata agagtaaagt acctatggta atacacttag atcatgggga aaataaagaa
     5821 caaatattaa gggcaataag atgtggattt acatcagtaa tgattgatgg ctcacattta
     5881 ccatatgaag agaatgtagc tatatctagg gaaatagtag aaatagcaaa aggattaaat
     5941 gtgtccgtag aaggagaact tggaacaata ggaacaacag gaacatcaag tgaaggtggt
     6001 actgatgaaa taatatatac agattcaaaa ttagctaagg attttgttga aaaaacagga
     6061 gtagacacac tagctattgc aattggtaca gcacatggta tatatccaaa aggttttaaa
     6121 ccagagctta aactagattt attaaaagaa ataagagaag tagtagatat acctttagtt
     6181 cttcatggtg gttcatcaaa tccagatgaa gaaatagcac aagcagttaa actaggagtt
     6241 tgtaaaataa atatttcgag tgatgttaag tcggcatatt ataagaagtg cagagaacta
     6301 ttagaacaaa acccaagctt atatgagcca gatactatat atccaccatg tataaaagct
     6361 gcaagagaag ttatagagtt taaaatgaat ctattcaatg ctatagataa gttaaagtgc
     6421 ttttataaaa ataaaaatta attatgaggt aacaaattaa aaggagggga gaatttatga
     6481 taggtaaaat actacgaagc gattgtattg tactagataa acaattacaa acaaaaaaag
     6541 atgtaattaa ttatatggca aatcatttgt accatggaaa ttatataaat aataaagaaa
     6601 agtttatagc tgatattaat aaacgtgaaa aagaatcatc aactaatatg ggtaatttaa
     6661 tagctatacc tcatgctaag tcaaatgtag taaatcaacc tgtaatgatg tatttaagaa
     6721 cagatagaga gattacagat aatgatggtc aaaaacttag gtcatttttt atgatagcag
     6781 ttccagaatc aggttcagat gaacatctta agattatatc taaactagct acatatctaa
     6841 tggaagaaaa ttttcttaag catctactaa gtgcagaaac tccatacgaa atattaagta
     6901 tatttaataa taaagctgca gaatcaggag attttagtaa taatcaaaat aaaggtctta
     6961 tagttgggat aacaggatgt ccaacaggta tagctcatac ttttatggca aaaaaagcat
     7021 tagaggaagc agggcaagaa cttggatatg aagttaaaat agaaactcat ggagctatag
     7081 gtgtagaaaa taagttgact aaagaagata taaaaagagc tgaatacgta ataatagctt
     7141 gtgataaaga agttcaactg agtagattta taggaaagaa agtgctacaa aagaaagtag
     7201 tagatgctaa gaatgtttca ttagctaaaa atttaattat aaacgctgaa aatggaatgg
     7261 ctaaagaaat taaaccaggt ggaaagactt tagaagattc aaaagaaatg cttcaagggt
     7321 cagagacaaa gggtgtttat aagcacttaa tgagtggtgt atctgaaatg attccatttg
     7381 tagtgatagg tggaattggt atagccatag catttatgtt tggtatattt gcatcagacc
     7441 caactcatga aacatacaac cctatagctg gattctttgg acaacttgga ggcgattcag
     7501 catttaagtt atttataccg atacttgctg gttttatagc taaatcaata gcaggaagac
     7561 aaggatttcc tcctgctatg atagctggtt ttatggcagt tattggaggt tcagggttta
     7621 taggtggtat ggtagctggt tttgcaacag gatatatagc tttaggagta atgaagataa
     7681 cagaaaattt accagatgta ttaaaaggag taaatgctgt attgatatgt ccacttttga
     7741 ttgcatttac gagtggagct ataatgttct ttgtagttaa tacgccagca tcatggttaa
     7801 atgaagcttt acaatcttgg ttacaaagta tgtctggaac taataaagta ttattaggtg
     7861 caatattagc agggatgatg gcatcagata tgggaggacc tattaataaa acagcttcag
     7921 cttttggatt ggctatgttt tcaagtaata tatttgagcc atcagcagca ttaatggtag
     7981 gaggaatggt acctccatta gcgatatcat tagctatgtt aatattcaaa aataagttta
     8041 ctaaagaaga aagaaagtca tctctaccaa ctgctataat gggggcatct ttcataacag
     8101 aaggcgcaat accgtttgca gctgcagacc cattaagaat gattccagca aatataattg
     8161 gttcttgtat aggtggggca atctgtatga gtttagatat aactttaatg gcaccacatg
     8221 ggggaatatt tgtaatacca tttgcatgta gtgcaccagt tgcatatata gcttgtatat
     8281 tgattggtac agtaataaca gcattgatta taggggtagc aaagcctact ataagaagtg
     8341 aaaacagtga agttgaatta cttggtgctt aaattataaa taatagtaaa aataagtaat
     8401 ggaataacaa caagtatata gaataataat aaggatattt agaatcagtt aactacttct
     8461 aaatatcctt aatttttatt ttgaaattat attatctatt atatcaattt agtaaggtgt
     8521 atcataaatt cttttatgat acagaagtat cttagtaatt ttacatagtt agtaaatatg
     8581 ttaaaatagt acttaatata aacatagaaa tatataaaca taggtaggga gggctaagta
     8641 tgcttgtgtc gaggcaaatt gaatttctta aattgatact aaacgaaaat aaatatagac
     8701 ctataaaata ctttaaagat aaattaaatg tatctgataa aactttacaa aaagatttga
     8761 agatgattga gaaatattta gaaactttta atataaaaat tgatataaaa agaggctatg
     8821 gaatattaat agaagattta gcaaagaaga acatagactt aattaatagt ttaaatatac
     8881 aatcgaagga aaataaatct ttatcagtag agcagagaag aatggagatt ttaaagtatt
     8941 tattactaaa ttcaaataat ataacttcaa taaatcagtt agcagataaa tattatgtaa
     9001 gtaaaacttc tatagtaaat gattttaaat atatagaggc atggataaaa gaatacaatt
     9061 taaaacttaa taaaacttta gaaggaacta agattatagg taaagaagtg gatataagga
     9121 aatcgattgc caaaatgatg gatgatttat tagatgaaaa ttatgaacaa catgatatat
     9181 ctgagttaac aagattagat tctgctacat ttagtgcatt gataaattta tttgatattg
     9241 attctattat atttgtagaa actataatta cagaattaga atacaattta ggatatacta
     9301 taaatcagcc ctactatata aatttaatta cccatatact tatatgttta aagagaatcg
     9361 aagaaggtaa tcaaatagaa tctcaggaag aaagagaaat ttctgtagat aatttggacg
     9421 agttagtata taaaaatgtc actacactta tagataaaat agaaaataga tacagagtaa
     9481 aaataacgaa agatgaaatt atgtacatat atattttttt ggtatcatct ggatttagta
     9541 gcgaaaataa tagtagttat aatgatgaat cagatatatt aagtgaaagt gaaaaagttt
     9601 catctataat gatagagaat atgtcacaat ttttaaatat aaatctcaaa aatgatgact
     9661 tactacaaaa aagtctagca tctcatgtaa ggcctatgtt aaacagatta agatatgata
     9721 tccaaataaa aaacccgttg ttaggagaga tacaagagcg attttcagat gtgctgggat
     9781 tatgtatgat gactataaat attatgacag agtatgataa tctgaagaat attagtatag
     9841 atgaagtttc gtatcttgcc acatattttc aagcggcaat agaaagaaat atgagtagta
     9901 aaagagtaat agtagtttgt catacaggat atggaacatc tcagctcctt gcagccagat
     9961 taaaaagaga atttcctgaa tggactatag ttgatatagt tcctatgcat cagttgagta
    10021 agcgaaattt agatgatgta gactttgtaa tatctactgt aaacttagat ttgaaagata
    10081 aattacatat agttgtatca gtactattaa tggaaagtga tattaataat ataaaaaatg
    10141 ctttactttc taaacctaaa aaaagtatga acttagatac aaacttatta ggaatgtatt
    10201 tagaagataa tatatatttt aattttgatg tagctcagat ggaaagttta ataggtgtga
    10261 atttagggag taaatatgaa tctatttcat taggaaaaga tttgaatata tatatacata
    10321 agcaaagtaa attaaataaa gggataatga atatcaataa tgttactaga acgattgata
    10381 tttatttaga aattagtaat caaagttttt taaagaatat tttaatggaa atatcaaatt
    10441 tatataggca aaagaactat ataaactata taatagattg caagagcaaa gaggatatta
    10501 aaatattatt taagaataat ctattggaga aaacaaatgg aaattagaga aatattaaca
    10561 gaagaaagca ttattttaga tttgaaatca actacaaaag aaggcataat acaggaatta
    10621 gtagataaat tatacgatac aggaagtata actttaaaaa gtaagtttat aaaggcatta
    10681 aaacatagag aaaaaatagg ctctactagt ataggttatc aaatggccat gccacatgga
    10741 aaaacaaaat atgtaaagaa ggcatccata gtaataggta aatctaaaaa aggtgtagat
    10801 tggccaagta tagatggctt acctgtaaag gtaataatta tgtttgctat gccacaatct
    10861 ggaggcaatg aagatatgat attaatatct aaaatagctt acattttagg tggtaaaaat
    10921 ataagtgaaa gacttttaaa tgtacataca aaaaaagaaa tattagatat attttatgaa
    10981 gtgtaggtat tgtataaatt ataaatttga ggtttttaaa ataaaatgtg tattctttta
    11041 aatagtatag attaattttt agacactaga gaggagagga tatctatggc aatatattta
    11101 gaaatgccta aattaataca tgaatttcct tttcgtagtc tatcaaatga aggagaagta
    11161 ttaacaacac cacactggca taaggaaata gaaatattac ttataacaga aggtgtagta
    11221 aatctattta taaatgacaa gcctatgcaa ttaaaaaaag gcgagatagt gataataaaa
    11281 ggaggagata ttcattatat actgccatca ccgggaagtg aaagattagt atttcaattt
    11341 gatatatctt tttttaatga tttaatttta ttaaatgagg aaaaagaaag cttaattaat
    11401 ttattcagta gtataaaaaa atgtagtaga gattggtcag caaaaataag aaagtcagtt
    11461 ttcgatatat tgatagatat ctacaatgaa gacctataca aaatagaagg atatagttat
    11521 gcaataaaag gtaaaatgta taatttaatt cctatattat atagacagat tcctagagaa
    11581 attgaagaaa atacagtaga atatgaaata aactctaaag agatattaga gagattgaac
    11641 aatatattca aatatgtaga aaaaaattat aaacaaccta taaaattaga agatgtgtct
    11701 tatgaagtag gatttagtgt atattatttt actaaatttt tcaaaaaaaa tacaggaaaa
    11761 acatttataa cttttttaaa tgaatataga ttagaaaaag caaagtggat attacttaat
    11821 aacaatatat ccattgtaga tttggttgaa gaagttggtt ttggaagtac aaaaactttt
    11881 tacagagtat ttaaagaaaa aattggagtc tctccattag aatttaaaaa aagatataat
    11941 agcttggaga aaaaaggggg tgcctcaaaa tgaacttagt tcatgaggca ctttctttta
    12001 tatgaaatca aatatatttt cagtatttca acaatgaaaa ttccattcat ttataaagtc
    12061 taaattttag ttctcaatac aatctctatt tcgaaataaa tccttttata tatataaata
    12121 tacagcaata tatgagaata agataacaat aatgaggata taaaaa
//
LOCUS       DAMJWP010000099        12132 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.103, whole genome shotgun sequence.
ACCESSION   DAMJWP010000099 DAMJWP010000000
VERSION     DAMJWP010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12132
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.103"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            41..916
                     /locus_tag="PC700_002217"
     CDS             41..916
                     /locus_tag="PC700_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HDF2581522.1"
                     /translation="MITFDVNGAIIRFDDTKNNYNKIRKIFKLYALEISKNFERDCLN
                     SFQNLKQISDRGLSLGEKYIEESFKKGIETIVSFGIITIDIDTFKSVYCEKYLDFKRL
                     FNNLNKTFIIPNKNKKNGHASFLEIESTIKKMSNYLYNDCFKIHYAVVDALIENRITA
                     VHSYIDEESIKKSNALFNNYKDGFIGKADECKVVKQIITLNPYREDVYEFLVKEDGDF
                     SKEIERLTEFLGYDIKEYKASLMDIYINELMESDENDVENAKEKIEKYARYIGCSDEN
                     LYVARVNAIYTFANA"
     gene            1277..2575
                     /locus_tag="PC700_002218"
     CDS             1277..2575
                     /locus_tag="PC700_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase/BAAT N-terminal
                     domain-containing protein"
                     /protein_id="HDF2581523.1"
                     /translation="MKVNVYNVESFVDERLKIMVSDLIPNTKLKISVKMEFPWCKGEE
                     FSSYGVFCSNEKGEVDLDLVEPIEGTYKADNCMGLIYSLEKSKTEGKNFAENISIDKP
                     IIMNMIFETSTERKEVELKRIFKSEDITTKYISDEFIGKLFYKQNSNNKTILMLGGSD
                     GNLDALDLLAAPLASRGFNVLTVAYFALEGLPDKLEEVPLEYFEKVFEWISENEITST
                     KEIFVHGTSKGGELAFLLASRYEQIKKVVVSQPHIYCFQALNGLMSGNDTSSWSYKGE
                     PFPYIKVDNDIFFEEQKKNISKGIPFGFNSTYKKSLKRAENRKEARIKIENSKADILM
                     IAGKEDNIWNSYEACLEALEIFEENNYPYNVELLTYNNMGHPLPILYIMPLKETLCMS
                     MSGGIFSSGGTVEGNSKGQFESWNRTIEFYKKLLSSDLNN"
     gene            2765..4156
                     /locus_tag="PC700_002219"
     CDS             2765..4156
                     /locus_tag="PC700_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HDF2581524.1"
                     /translation="MERLARREQPHIKFGMFKIRIPFIHYRFEKPEAIQGIISSMTSL
                     GTIGLSTQILGLPYEVAWSMAIINSILYCLHVFMGDPVVPGWITASITLTTAYLLKFS
                     MGIERIQALTALQIDLGIIFILMGITGVAGKLVSKIPNSIKGGILMGVGISTIISEFN
                     PKGRFDSYPISITVGILVACFVMFSERFDTLKVKNKFLFHLGEYGVVSAILVSLIVGI
                     FSKEIEIPRFSFDNLVYIVDFKNLINTVSPFGIGFPSVMLFIQGIPMAFMIYIIAFGD
                     FITGENLVLSESENRKDEYIDFNSNRSNVISGIRNIFMAIFSPYIPMCGPLAATLTGS
                     VAQRYKVGKEAMQSVFSGMGTLVWVSAIFICFYPIAQIATPLIPLALSVTLLVQGYLC
                     TKLSMELCNTGVEKGLIGLMGGVIAAKGGTWGLAVGFILYFILIDSKKRKEKDINSIE
                     DCVYEILDKKKSI"
     gene            4333..4920
                     /locus_tag="PC700_002220"
     CDS             4333..4920
                     /locus_tag="PC700_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2581525.1"
                     /translation="MPPKVKITKEMILNTVLDITREVGFEAVNARSIASKLQCSTRPI
                     FTCYENMNELKNEFLAFAYEYYNQYVVNYRNSKSVSPYLLLPLSYIEFAQEETYLFKL
                     LFINDLDLMMKEANDFYKEIDNEKKARLFSESIGIELECAKVIFLDLFLYTHGIAVLT
                     ATKKLTLDRYSAEKMLKNILTAFIRQEKPDWNLSI"
     gene            4953..5744
                     /locus_tag="PC700_002221"
     CDS             4953..5744
                     /locus_tag="PC700_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2581526.1"
                     /translation="METKQYLIDFYNTYDEDSRLALKHGMVEFLTTMHYIDKYIKSGD
                     CVLEIGAATGRYSHTLARQGYDVDAVELVEHNIEVFHKNTQSNENISITQGNAMDLSV
                     FPDNKYDITLLLGPLYHLYNKEDKQQALHEAIRVTKPGGVVFAAYVISDGCLIDEGFH
                     RGNINVSEYIEKGLIDPQTFAAKSEPKDLFELVRKENIDDLMSAFNVTRLHYVASDGL
                     ALYMREAVDSMDDDAFALYLKYHLATCEREDLVGVTSHAIDIFRK"
     gene            complement(5925..8342)
                     /locus_tag="PC700_002222"
     CDS             complement(5925..8342)
                     /locus_tag="PC700_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2581527.1"
                     /translation="MFRNNNRTFIRKIALNDLKINKLKTYLSGIIIMISTCLLLTVTL
                     VSYNASVDLVNASPYHAIYKSVDEKAKNILYKDKNLSGVGTYKLIGSNKKTDCIMSIV
                     YADDTAIKLMNFQPLKGKLPTNKNEIAISEKYLQEFGLDKDIGDSIKLNYYDDITNKE
                     VQCDFIIVGFLENYYQDNAKQYYTVVSNDYFKNIASTPLQNKSNTFDESRPDTVDVLV
                     KLSEENTEKDSASIKTQLKKIGLSLGIKKYNIYLNDNYIESNLIDGEQIITVLVVGIV
                     VLFSSVFVIYSIFYISVVNSVQMYAKLKSLGMTSFQLKKTISLQGNILSIIFIPLGAI
                     ASCIIAYIIQPLAWQMIADLFIILILSLVMFLTVRISLFKPARIISKISAIEAMQYTE
                     TKFRKQAKNFSYINIKNLALKNIESNRKKNLIALISLSISGVLFISIANLANSIDFKK
                     QLSQQFVYNEDYIISIKSDNLYEHITEIQQNNPLTDSLKNEIKSIHGVKKLIESKSIK
                     SNIIEPYIKDRDEKQFITLIKGITPELSKSLEEYIVSGKIDYTHLDSDSLIINKYRTK
                     SYGLNLKVGDTVTFNIFSGNDIIKKKMKIIGIIDNSNTGGMFFASDKTLESLTPYNTN
                     LDFSILIDKKHSKEVEEKLLSIIQKNSNLMLYSYEDDYRMITRAFQYIIIASYIFVGL
                     ISCFGILNMTNTLINSVLIRKKEFALLQAVGMTRKQLRNMLYREGLNISIKAICTSSI
                     LGYFGSNLLCTFIKDVIRLDFINFKFSIFTILIFSFVLIGIQVLVTELLIRNIEKKSL
                     TERLRSQ"
     gene            complement(8355..9020)
                     /locus_tag="PC700_002223"
     CDS             complement(8355..9020)
                     /locus_tag="PC700_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2581528.1"
                     /translation="MSILQVINLKKYYGQSSNITKALDGVTLSIEKGEFISIVGTSGS
                     GKSTLLNMMGGLDNPTSGKVIIKGKELSTMTDEQLTIFRRRNIGFIFQNYNLVPLLNV
                     YENIILPIGLDGETIDKDFINEIINMLSLEEKLYDMPSNLSGGQQQRVAIARALVTKP
                     AIILADEPTGSLDSRTSSDVLSLLKVTSKKFNQTIVMITHNNEIAQLTDRIIRIEDGK
                     IVN"
     gene            complement(9102..10133)
                     /locus_tag="PC700_002224"
     CDS             complement(9102..10133)
                     /locus_tag="PC700_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2581529.1"
                     /translation="MNFKKFSFSSIYLIIAVGIIFTAILFNISIYLITDDLKLIALLL
                     IYTIVLCGFIALIIFLLHKKIVIFCSKICHTLDSMMNSEDNIEIELEEETILSRINHR
                     LIRMYEVIQENRNSIAIEKADLQELVSDLSHQIKTPIANLKMINTTLLNQTFKTEIQK
                     DFLMDMENQLDKLDFLMQSMVKTSRLETGIITLSKKKNSIYETIATALSGILFDAEKK
                     NIEVTVDCDPNLYIIHDKKWTSEAIFNILDNAVKYTNSNGKIRVATECWVMYTKIDII
                     DNGKGISESHQAEIFKRFYREEDVHDIEGIGIGLYLSRKIISLQGGYIKVTSDIGKGS
                     TFSIFLPNK"
     gene            complement(10123..10809)
                     /locus_tag="PC700_002225"
     CDS             complement(10123..10809)
                     /locus_tag="PC700_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2581530.1"
                     /translation="MEKILVVEDDSILNKTLSYNLIEDGYIITSKFTAKSALKSIFEC
                     EFDLIILDINLPDKSGFELCNEIKENYNIPIIFLTANDMECDMIKGYELGALDYITKP
                     FNINLFKQKVKAFLNHLTTKTKQDYYRDGYLEINFSELSANINGNQIIFTPLEYRTLK
                     LLTENPKSILTRKVLLEKLWDIDANFVDEHTLTSVISRIRSKIEKDNLKYIKTVYGMG
                     YMWLGERNEF"
     gene            complement(10967..12031)
                     /locus_tag="PC700_002226"
     CDS             complement(10967..12031)
                     /locus_tag="PC700_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HDF2581531.1"
                     /translation="MKRVDERNTMFARANYKKDSTAYNDYYKKNPDKKEIDDSIRNRP
                     NLCSEGTMTYNELNSPMASSAFDFLSDIKSLCEGKVSDTKVDVDAKSMTKKIKGLAKQ
                     YGASVVGITKLKDYHIYTNRGRHEENYGEEINLTHKYAIVFGCEMDKEMINRAPMICE
                     VIETSKCYVDASIVGMILSYYIRNLGYDARNHMDANYLVMPVFIARDAGLGDIGRNAI
                     LTNKDYGSRLRLGVVTTDIPLLEDEYVDFGLEDFCKVCKKCSFNCPSHSLSNDIKIGD
                     DGKYNWVIEHETCYIKWRYLGTDCGMCISACPFSQNLETIKNTTSFKGNNELIQKALD
                     EYTSKFGKRIFIPGNPSWLK"
BASE COUNT         4396 a         1540 c         1740 g         4456 t
ORIGIN      
        1 tttattttat tgatgaaact aatggggagg ttaaaactca atgattactt ttgatgtaaa
       61 tggagctatt atcagatttg atgatacaaa aaataattat aataagataa ggaaaatctt
      121 taaattatat gctttagaaa tatctaaaaa ctttgagaga gattgtttaa atagttttca
      181 aaatctaaag caaatatctg atagaggttt aagtttagga gaaaaatata tagaagagtc
      241 ttttaagaaa ggaattgaga ctatagtaag ttttggaatt ataactatag atatcgatac
      301 ctttaaaagc gtttattgtg aaaaatattt agattttaag agacttttta ataacttgaa
      361 taaaactttt attataccaa ataaaaataa aaaaaatggg cacgctagct tcttagaaat
      421 agaatcgaca ataaagaaaa tgagcaatta tttatacaat gattgtttta aaattcatta
      481 tgctgttgta gatgccctta tagaaaatcg tataactgcg gtacattctt atattgatga
      541 agaatctata aaaaaatcta atgccttatt taataattat aaagatggtt ttattgggaa
      601 ggctgacgaa tgcaaagtag taaaacaaat aataacttta aatccatata gggaagatgt
      661 atacgaattt ttggtaaaag aagatggaga ttttagtaag gaaattgaga gattgactga
      721 atttttaggc tatgatataa aagaatataa agcgtcatta atggatattt atataaatga
      781 gttaatggag agcgatgaaa atgatgttga aaatgcaaaa gaaaagattg aaaaatatgc
      841 tagatatatt ggatgtagtg atgagaattt atatgtagca agagttaatg ccatatatac
      901 ttttgcaaat gcataatttt attaaacatt aaatattaga tgttcaatta tataaattta
      961 aataaaaaaa tatccttatt aataacaaat agtttatatt aaaaaccatt cttaaaatga
     1021 gtggtttttt attttgaata gatatatttt aaacctaata gcattcctat ctttaattag
     1081 ccaaaaatca actggatttc atatagacta cataatttta aattactaaa aaagacaata
     1141 tattgacaaa aatgaaatat gttgataaaa tgaaatagtc aatatggtgt cttaatttaa
     1201 aaatttattt atgttgtatt agataaagtt gttttatata taaagcataa ataattatta
     1261 taaggagaaa tagattatga aagtaaatgt atacaatgta gaatcatttg tggatgaaag
     1321 acttaagatt atggtttcag acttaattcc aaatactaaa cttaaaataa gtgtgaaaat
     1381 ggaatttcca tggtgcaagg gagaagaatt ttcatcttat ggagtgtttt gttcaaatga
     1441 aaaaggagaa gtagatttag atttagttga acctatagaa ggtacttata aggctgataa
     1501 ttgtatgggg ttaatttatt cgctggaaaa atctaaaact gaaggtaaaa attttgcaga
     1561 aaatatatct attgataaac caataattat gaatatgata tttgaaactt ctactgaaag
     1621 aaaagaagtt gaattaaaga gaatatttaa atcagaagat attaccacaa aatacatatc
     1681 tgatgagttt ataggaaaat tattttataa acaaaattct aataataaaa caattttaat
     1741 gctaggtggt tctgatggaa atttagacgc tctagacctt ttagcagctc cacttgcatc
     1801 tagaggattc aatgtactaa cagttgcata ctttgcttta gaagggttac cagataaatt
     1861 agaagaggtt ccattagaat attttgaaaa agtatttgag tggattagtg aaaatgaaat
     1921 taccagtact aaagaaatat ttgtacatgg gacatcaaag ggtggagaat tggccttttt
     1981 acttgcttca agatatgaac aaattaaaaa ggttgtagta tcacaacctc acatttattg
     2041 tttccaagct cttaatggat tgatgagtgg aaatgatact tcatcatggt catataaagg
     2101 agagccattt ccatatatca aagtagacaa tgatatattc tttgaagaac agaaaaaaaa
     2161 tatatcaaaa ggtattccat ttggatttaa tagtacttat aagaaaagtc ttaaaagagc
     2221 agagaataga aaagaggcta gaataaaaat agaaaattcc aaagcagata tacttatgat
     2281 tgctggaaaa gaagataaca tatggaatag ttatgaagct tgtttagagg cattagaaat
     2341 ctttgaagaa aataattatc catataacgt agaattatta acatacaata atatgggtca
     2401 tccgttacca attctatata ttatgcctct aaaggagact ttatgtatga gtatgagtgg
     2461 agggattttt tcttcaggtg gaactgtaga aggaaattca aaaggacagt ttgaatcatg
     2521 gaatagaact attgaatttt ataaaaaact tttatcaagt gatttaaata actaatatta
     2581 agtgatttaa gtaactaaaa atgaagcggt ttttctatat atgcacaaaa tctatcaatt
     2641 ttatagattt aatttattgt ttatataatt tgcatgttat atactaattt taatgacatt
     2701 gacaatttat aaacaattat aaagttgtaa acttataaga gttattgaaa ggtggagtga
     2761 agttatggaa agactcgcta gaagggaaca acctcatata aaatttggaa tgtttaagat
     2821 aagaattcca tttatacatt acagatttga gaaaccagaa gctattcaag gtataatatc
     2881 aagtatgaca agtttaggca caattggcct tagtacacaa attttgggat taccctatga
     2941 agtggcatgg agtatggcta ttattaactc aatattatac tgtttgcatg tttttatggg
     3001 agacccagtt gtacctggct ggattacagc ttctataact cttacaactg catatttgtt
     3061 aaagttcagc atgggcattg aaagaataca agcattaaca gctttacaaa tagatttagg
     3121 aataatattt attttaatgg gtataactgg tgtagctgga aagttagtta gtaagatacc
     3181 aaattctatt aaaggtggaa tactaatggg tgtcggtata tcgactataa tatcagagtt
     3241 taatcctaaa gggagatttg attcttatcc tatatctata acagtaggaa ttttggttgc
     3301 ttgttttgta atgttttctg aaagatttga tactctaaaa gttaagaata aatttttatt
     3361 tcatttagga gaatatggtg tagtatcagc tattttagtg agtttaatag taggtatatt
     3421 tagtaaagaa atagaaattc ctagatttag ttttgacaat ttagtttaca tagtagattt
     3481 taaaaatctg ataaatacag tatctccttt tggaataggt tttccaagtg taatgttatt
     3541 tatacaaggg ataccaatgg cttttatgat atacataatt gcctttggtg attttataac
     3601 aggtgaaaac ttagtacttt ctgaaagtga aaatagaaaa gatgaatata tagatttcaa
     3661 ttcaaatagg tctaatgtta taagtgggat aagaaatatt tttatggcaa tcttttctcc
     3721 atatatacca atgtgcggtc cattagctgc gacacttaca ggaagtgttg ctcaaaggta
     3781 caaggttgga aaggaagcca tgcagtctgt ttttagtggc atgggtacat tagtatgggt
     3841 ttcagcaatt tttatctgtt tttatccaat tgcgcagatt gctacacctc ttataccatt
     3901 agcattgtca gtaacacttt tagttcaagg atatttatgt acaaagctat caatggagct
     3961 atgtaataca ggtgttgaga agggattgat tggacttatg ggaggtgtaa ttgctgctaa
     4021 aggcggaaca tggggacttg ctgtaggttt tatactgtat ttcattttaa ttgattctaa
     4081 aaaaagaaaa gaaaaagata ttaattcaat agaagattgt gtatatgaaa tattagacaa
     4141 aaagaagagt atttaaagga aaatatgagt tgctgttatc tattaataga ttaattgcaa
     4201 ctcatgtttt atttataagt tttaggaatt gcaaaaaaat gcaaagcttt ttcttgacat
     4261 aatttcttga tatgttacta tagttatata acatatgtgt tttataaaaa tttaatggag
     4321 aggtgaacat atatgccacc taaagtaaag ataacaaaag aaatgatttt gaatacagtc
     4381 ttggatatta caagagaagt gggatttgaa gctgtaaatg cgaggagtat tgcaagtaaa
     4441 ttacagtgtt ccactcgacc aatttttact tgttacgaaa acatgaatga attaaaaaat
     4501 gaatttcttg cttttgcata tgaatattat aatcagtatg ttgttaatta tcgtaattct
     4561 aaaagtgtaa gcccttattt actgcttccg ctttcataca ttgaatttgc tcaagaagaa
     4621 acgtatttat ttaagttgtt atttataaat gatttagatt tgatgatgaa agaggcgaat
     4681 gatttttata aagaaattga caatgaaaaa aaggcaagac ttttttcgga aagcattgga
     4741 atagagttgg aatgtgcaaa agtaatattt ttagatttat ttctttatac tcatggcata
     4801 gctgtcttaa cagcaacaaa aaaattaaca ttagatagat atagtgctga aaaaatgttg
     4861 aaaaatatat taacagcttt tataagacaa gaaaaaccag attggaattt gtctatttga
     4921 tgtttattaa ctaatataga agaggtgaga ttatggaaac aaaacaatac ttaattgact
     4981 tttataatac ttatgatgaa gatagtcgac ttgcattaaa acatggaatg gtggaatttc
     5041 tcactactat gcattacatt gataagtaca taaaatcagg tgattgtgtg cttgaaattg
     5101 gcgctgcaac tggtcgttat tctcacacac tggcacgtca gggatatgat gttgacgcag
     5161 tggaattagt cgaacataat atagaggttt tccataaaaa tactcaatca aatgaaaata
     5221 taagcattac tcagggtaat gctatggatt tatctgtttt cccagataat aaatatgata
     5281 ttacgttact gttaggacca ctatatcatc tttataacaa agaagataaa caacaagctt
     5341 tacatgaggc aattcgtgta acaaaacctg gtggtgtggt ttttgctgca tacgttatat
     5401 ctgatggctg tctcattgac gaggggttcc atagaggaaa tattaatgta tctgaatata
     5461 ttgaaaaagg tttgattgat ccacagacat ttgctgctaa gtctgaacca aaggatttgt
     5521 ttgagcttgt acgtaaagaa aacattgatg atttgatgtc tgcatttaat gtaactagac
     5581 tgcattatgt cgcatcagat ggtttggctt tgtatatgcg tgaagcagta gactctatgg
     5641 atgatgatgc atttgcgtta tatttaaaat atcaccttgc tacttgtgaa cgtgaagatt
     5701 tagtaggagt tacaagtcat gcaattgaca tatttagaaa ataaggaata tgcttgttta
     5761 tgtctctggt gatgttgctt ggagatataa ggaagtgtat ctgtataaaa taaaaacaag
     5821 tagagggaca gaaaattatg taaagtcaaa attttctatc cttttagctt ttaaaaaatt
     5881 atgttaaaac ttgtataaaa aatggttatt ttttatatac attcttactg agaacgtaag
     5941 cgttctgtaa gtgatttttt ttctatattt cttataagta attcagtcac taaaacttgt
     6001 ataccaatta atacgaatga aaaaattaaa atagtaaata tagaaaattt aaaattaata
     6061 aagtctaatc taattacatc ttttataaaa gtgcaaagta gatttgaacc aaaataacct
     6121 agtatacttg aagtacatat agctttgata cttatgttta gaccttctct ataaagcata
     6181 tttcgcagtt gctttcttgt cattccaacg gcttgaagta aggcaaactc tttttttcta
     6241 attaaaacgc tgtttattag ggtattggtc atatttagta taccaaagca agaaattaaa
     6301 ccaacaaaaa tgtatgaagc tataattata tattgaaatg ctctagtaat cattctataa
     6361 tcatcttcat atgaataaag cattaaattt gaatttttct gaattatact taacaacttt
     6421 tcttcaactt ctttagaatg ctttttatct attaaaatag aaaaatctaa atttgtatta
     6481 tatggtgtta gagattctaa agttttgtct gaagcaaaaa acatacctcc tgtattggaa
     6541 ttgtcaatga ttcctataat tttcattttc tttttaataa tatcatttcc actaaagata
     6601 ttaaatgtaa ctgtatctcc aacttttaaa tttaatccat aagattttgt tctatattta
     6661 tttataatta aactatctga atctaaatgt gtatagtcta tttttccact gacaatatat
     6721 tcttcaagtg attttgaaag ttcaggcgta atacctttaa ttagagtaat aaattgtttt
     6781 tcgtctctat ctttgatgta aggttctata atattacttt taattgattt ggattcaatt
     6841 aattttttta ctccatgaat agatttaatt tcattcttaa gagaatctgt tagtggatta
     6901 ttttgttgga tttctgtaat atgctcatat aagttatcac tcttaatgct aattatataa
     6961 tcttcgttat agacaaattg ttgagatagt tgttttttga aatctattga attagcaaga
     7021 ttagcgattg aaataaataa aactccacta atgcttagcg atattaatgc aatcaaattt
     7081 ttctttctat ttgactcgat attctttaat gctagatttt ttatatttat ataactaaaa
     7141 ttttttgctt gttttctaaa tttagtttct gtatattgca tggcttcaat agcagatatt
     7201 ttagaaataa ttctagctgg tttaaataaa gaaattctaa cagttaaaaa cattaccagt
     7261 gataaaatta aaattataaa taaatcagct atcatttgcc atgctaatgg ttgaattatg
     7321 taagcaatta tacaagaagc tatagcccct aatggaataa aaataattga caaaatattt
     7381 ccttgtagag aaatagtctt ttttaactga aaagaagtca tacctaacga tttcaactta
     7441 gcatacatct gaactgaatt aacaactgaa atataaaaaa tactatatat aacaaaaacg
     7501 ctagaaaaaa gaacaactat tccaactact aaaactgtaa taatctgctc accatcaatt
     7561 aaatttgatt caatatagtt atcatttaag tatatattat atttcttaat acctaaagat
     7621 aaaccaattt tctttaactg tgtcttaatt gaagctgaat ctttctctgt attttcttct
     7681 gatagtttaa ctaatacatc tacagtatca ggtcttgact cgtcaaaagt attactttta
     7741 ttttgtaaag gtgtagaagc tatattctta aaataatcat ttgaaacgac tgtatagtat
     7801 tgcttggcat tatcttgata ataattttct agaaatccaa caataataaa atcacattga
     7861 acttctttat tcgtaatatc atcataataa tttaatttaa tgctatctcc aatatcttta
     7921 tctaaaccaa attcctgtaa atatttttct gaaatagcaa tttcattttt atttgtaggt
     7981 agtttgcctt tcaatggttg aaaattcata agtttaattg ctgtatcatc tgcataaaca
     8041 atcgacataa tacagtctgt ctttttatta cttccaatta atttgtaggt tcctacgcca
     8101 ctaaggtttt tatctttata tagtatattt tttgcttttt catctacaga tttatatata
     8161 gcatgatatg gagatgcatt aactaaatct acagaagcat tatatgaaac taaggtcact
     8221 gttaataata aacatgtaga aatcattata atgatacctg aaagatatgt tttaagttta
     8281 ttaattttta aatcatttaa agctattttt cttataaatg tgcgattatt atttctaaac
     8341 atatactaca cctcctagtt aacaattttt ccatcttcaa tacgaataat tctatcagtt
     8401 agttgtgcaa tttcattatt gtgtgtaatc attacaattg tttggttgaa ttttttactt
     8461 gtcactttaa gtaaactcag tacatctgaa ctagttctac tatcaaggct acctgtgggt
     8521 tcatcagcta gtataatagc tggttttgtt acaagtgctc tagctatagc aacacgttgc
     8581 tgttgaccac cagaaagatt acttggcata tcatataatt tttcttcaag agaaagcata
     8641 tttataattt catttataaa atccttatct atggtctctc catccaatcc tattggtaag
     8701 ataatatttt catatacatt aagtaaagga acaagattat aattttgaaa tataaaacct
     8761 atatttctgc gtctaaagat tgtaagctgt tcatcagtca tagtagataa ttccttaccc
     8821 tttataataa cctttccaga tgtaggatta tctaatcctc ccatcatatt taataatgtg
     8881 gatttgccac taccagatgt acctacaata gaaataaatt cgcctttttc aatagataga
     8941 gttacaccat caagagcttt tgtaatgttt gaactttgtc cataatattt ctttaaatta
     9001 attacttgta aaatactcat aataatacct ccttaacatc ttgtaaatac agtataaaat
     9061 caaaatgttg cagaatcatt acaaataaaa tatttaaaaa tttatttatt aggtaagaag
     9121 atagaaaaag tcgacccttt tccaatatct gatgtaactt taatatagcc accttgaaga
     9181 gatataattt ttcttgaaag atatagacct attccaatac cttctatgtc atgtacatct
     9241 tcttcacgat aaaatctttt aaagatttct gcttgatgac tttctgatat tccttttcca
     9301 ttatcaataa tatctatttt tgtatacata acccaacatt ctgtagctac acgtatctta
     9361 ccattagaat ttgtgtattt tacagcatta tctaatatat taaaaatagc ttcagaagtc
     9421 catttcttgt catgaattat atataaattt gggtcacaat caactgtaac ttcaatattc
     9481 tttttttcag catcaaacaa aattccacta agtgctgtag caattgtttc ataaattgaa
     9541 tttttctttt tagatagtgt gattatacct gtctctagtc tggaggtttt taccatagat
     9601 tgcattaaaa aatctaattt atctaattgg ttttccatat ccattaaaaa atccttttga
     9661 atttctgttt taaatgtttg atttagtaga gtagtattaa tcatttttaa atttgcaata
     9721 ggagttttaa tttgatgaga tagatcagaa actagttctt gtaaatcagc tttctctata
     9781 gcaatactat tgcgattttc ttgaataact tcatacattc taattaatct gtgattgata
     9841 cgtgataaaa tagtttcttc ttctaattca atttcaatat tgtcttcact attcatcata
     9901 ctatctaaag tgtgacatat tttagaacaa aatataacta tttttttatg aagtaaaaat
     9961 attattaatg ctataaatcc acaaagtact attgtatata ttaataatag agcaattagt
    10021 tttaaatcat cagttattag ataaattgaa atattaaata aaatagctgt aaaaattata
    10081 ccaacagcaa ttattaaata aatagaacta aaagaaaatt ttttaaaatt catttctttc
    10141 acctaaccac atatatccca taccatatac cgttttgata tacttaagat tgtctttttc
    10201 aattttgctt ctaatcctac ttataacaga tgttaaagta tgttcatcta caaagttagc
    10261 atctatatcc cagagttttt ctaaaagtac tttccttgtt aaaatacttt taggattttc
    10321 agtaagcagt tttaaagtac gatattctaa tggtgtaaat ataatttgat ttccatttat
    10381 attggcagat aactcagaaa agttaatttc aagatatcca tccctatagt aatcttgttt
    10441 tgtttttgtt gtaagatgat ttaaaaaagc tttaactttc tgtttaaata aattgatatt
    10501 aaatggtttt gttatataat ccaaagctcc aagttcatag cctttaatca tatcacattc
    10561 catatcatta gcagttaaaa aaattattgg tatattgtaa ttttccttta tttcattaca
    10621 taattcaaac ccacttttat ctggtaaatt aatatccaaa attattaaat caaattcaca
    10681 ttcaaaaata gactttagtg cagatttagc tgtaaatttt gaagtaatta tatatccatc
    10741 ttcaattagg ttatatgata gagttttatt taatatagaa tcatcttcta ctactaatat
    10801 tttttccata cagttactcc ttttagttta atattttata atgtcaatta tagactaaaa
    10861 acattgctga attgttataa acgagaaaaa gtatttaaaa atttatatat ttaataaaga
    10921 acctgtccta tgataaattc tagtacagat tctttattaa ataaaattat ttcaaccaag
    10981 atggatttcc aggtataaat attcttttac caaattttga agtatactca tctaaagctt
    11041 tttgaataag ctcattatta cccttaaatg aagtagtatt ctttatagtt tcgagatttt
    11101 ggctaaatgg gcaagcagat atacacatac cacaatctgt acctaaatat ctccatttaa
    11161 tatagcatgt ctcatgttca attacccaat tatattttcc atcatcacca attttaatat
    11221 cattagaaag cgaatgagaa gggcagttaa aagagcattt tttacagacc ttacaaaaat
    11281 cttctagtcc aaaatcaaca tattcatctt ctaaaagagg aatatctgta gtaacaacac
    11341 ctaatctaag tctagagcca taatctttat ttgtaagtat agcatttctt cctatatctc
    11401 ctaaacctgc atcacgagct ataaaaacag gcattactaa ataattagca tccatatgat
    11461 ttctagcatc atatcctaaa tttcttatat agtaacttaa aatcatgccg acgatagatg
    11521 catccacata gcactttgat gtttctataa cttcacatat cattggagct ctatttatca
    11581 tttctttatc catctcacat ccaaatacaa ttgcatactt atgagttaga ttaatttctt
    11641 caccgtaatt ttcttcatgt ctacccctat ttgtataaat atgatagtct tttaatttag
    11701 ttataccaac tacagaagct ccatactgct ttgcaagacc cttaattttt tttgtcattg
    11761 actttgcatc tacatctact ttagtatcag aaacttttcc ttcgcataaa gatttgatat
    11821 cagataaaaa atcgaatgca ctacttgcca ttggagagtt taactcattg taagtcattg
    11881 ttccttcact acataaattt ggtctatttc ttatactatc atcaatttct tttttgtcag
    11941 gatttttctt ataataatca ttataagcag tactatcttt tttgtaattt gctcttgcaa
    12001 acatagtatt tctctcatca acccttttca attctattcc ccctagtaaa aatatattta
    12061 aaaaatataa ctttattgtt gtataattta attatataag aataacgtta tattttcaat
    12121 ttaagcgtaa tt
//
LOCUS       DAMJWP010000100        11891 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.104, whole genome shotgun sequence.
ACCESSION   DAMJWP010000100 DAMJWP010000000
VERSION     DAMJWP010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11891
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.104"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            155..1468
                     /locus_tag="PC700_002227"
     CDS             155..1468
                     /locus_tag="PC700_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2581532.1"
                     /translation="MHTQLQKTIGLSAALSTVVGMVIGSGVFFKPQAIYTTTNGAPGL
                     GIIAWLLGGFITITAGLTATEISAAIPKTGGMMIYIEEIYGEKLGFLTGWMQTVLFFP
                     GTSAALGVIFAQQASELLGMSPNNMANVLPIAIGVILFLALLNIIGSSLGGKVQTVAT
                     IGKMIPLILIIVFGFIKGQSSEVLNPFVGDGVNASNALGQVLIATLFAYDGWINVGAI
                     SGEMKSPEKDLPRAIVGGLSLVMAVYIIINIAYLWVVPASELATVTSPATLVATRLFG
                     NIGGKVITVGILISVFGTLNGYLLTGSRIPYTLAEMGTLPASKTLLKVNSGGSPVNSI
                     LLITVLACVYALSGQFNLLTDLTIFSIWVFYVLTFIGVMRLRRQKPDLHRPYKVPLYP
                     IIPIIAILGGLFVIINQILTSTIISLGGIFITLLGLPVYYYMKKR"
     gene            1640..2815
                     /locus_tag="PC700_002228"
     CDS             1640..2815
                     /locus_tag="PC700_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDF2581533.1"
                     /translation="MLGLYVHIPFCVKKCKYCDFNSYKMDIDSKKRYIEDLKIEMKLY
                     SNKLYKDNKYKNKECCSLNKNDKITSIFVGGGTPSILTSDEIREVFISIKEMFDIDEN
                     AEITIECNPGTLTLEKLKTMKEIGINRLSIGLQAIQEKHLNFIGRIHTYEEFEKNYKD
                     ALSVGFKNINVDLMYSLPNQTLCDWKETLEKVVHLNPTHISAYSLILEEGTELYNMYE
                     SNKFELIDENVDIEMYEYTINYLKSKGYNQYEISNYSKEGYNCEHNILYWECEHYIGI
                     GAGASGYINENRYNNVESLEDYHLSLVKREKPIQENEILSEKDMIEEKIFMGLRMNKG
                     IKFEDFKKKFGIDFREKYNKQIEMLLARKLINQSFEGIQLTQKGREISNSVFIEFME"
     gene            2918..3937
                     /gene="hrcA"
                     /locus_tag="PC700_002229"
     CDS             2918..3937
                     /gene="hrcA"
                     /locus_tag="PC700_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HDF2581534.1"
                     /translation="MELNERKLNILKAIVKDYIETAEAIGSRTISKRHDLGVSAATIR
                     NEMADLEELGYLIQPHTSAGRVPSEKGYKLYVNSLMSKSELDDNDKILIEQCMNHNIN
                     HIKELIHETSKLLSQLTNYTTVAVTKSLINQSVIKHIQLVAMNDNNILLIVVTDKGDL
                     KKANLTTNVYLDQSKLNLISDNLTRKLLGKSITDLDDNLIAFIKYEISEYSGLIDELL
                     NALNSNMKEEDFSLSLNGATNIFSYPEFNDVLKAKSFLNMLEKKETIADIIKSKGIQK
                     DNLNIIIGSDNDCELAQDCSIVTATYNVDRDLVGRISFIGPTRMDYARIYSIINYMSL
                     LINRK"
     gene            3967..4587
                     /gene="grpE"
                     /locus_tag="PC700_002230"
     CDS             3967..4587
                     /gene="grpE"
                     /locus_tag="PC700_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDF2581535.1"
                     /translation="MDKKNEQQEVREENDTSINQESETQVELEEEVVNEECETSSEKT
                     DEKEVDDENVTDINSKLAEKKLQDELDELNDKYQRLQAEYANYRRRTQQEKETIGVFA
                     NEKIITELIPVIDSMERALDACEDKEDTMYKGISLVHKQLIDTLVKFGVEEIEAESKE
                     FDPNLHLAVMQESVDGVEANQIVMVLQKGYKLGTKVVRPSMVKVSC"
     gene            4679..6526
                     /gene="dnaK"
                     /locus_tag="PC700_002231"
     CDS             4679..6526
                     /gene="dnaK"
                     /locus_tag="PC700_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDF2581536.1"
                     /translation="MGKIIGIDLGTTNSCVAVLEGGEAQIIANSEGMRTTPSVVAFTK
                     DGERIVGEPAKRQAVTNADKTITSIKTHMGTDYKVNIDGKSYTPQEISAIILQKLKSD
                     AESYLGQTVTEAVITVPAYFTDAQRQATKDAGRIAGLDVKRIINEPTAAALAYGMDKL
                     DQEKKILVFDLGGGTFDVSILEIGDGTFEVLATAGNNRLGGDDFDQIVIDYLADEFKK
                     AEGVDLRNDKMALQRLKEAAEKAKKELSSTMSSNINLPFITATAEGPKHLNIDLSRAK
                     FEELTRGLVEKTMEPTKTALQDAGLSTGDIDDVLLVGGSTRIPAVQEAVKKFIGKEPH
                     KGINPDECVAAGASIQAGVLAGDVKDLLLLDVTPLSLGIETMGNVMTKIIERNTTIPT
                     KKSQIFSTAADNQTAVDIHVLQGERSMAYDNTTLGRFQLTDIPPAQRGIPQIEVTFDI
                     DANGIVNVSAKDLGTGKEQKITITSNTNLSEAEIEQKIKEAEMNAEADKQKKEKIEAF
                     NQAESTIYQTEKTLNELGDKISSGEKEDIEKAIADLKAVKDNQDATAEELKKATDEVM
                     TKFQKVSQEMYQKAAQEQQAAQGAEQAQDNGPKDDNVVDADFKEVDEDK"
     gene            6737..7891
                     /gene="dnaJ"
                     /locus_tag="PC700_002232"
     CDS             6737..7891
                     /gene="dnaJ"
                     /locus_tag="PC700_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDF2581537.1"
                     /translation="MSTKRDYYEVLGISKDAEAQEIKKAYRKLAMKYHPDRNPGDKEA
                     EEKFKEINEAYEVLSDDTKRKTYDQFGHDGLNGQGGFGGQGGFGGQGFGGFEDMFGDI
                     FGDMFGGSFGGGRARRRGPQRGADIRQSVTISFEEAAFGKKMSIKVNRSEECEECNGT
                     GAKPGTSKKTCSTCNGTGQVRTVQRTPFGNIASSRPCSACNGTGEVIESPCSKCHGTG
                     NTRKVKTIEVDIPAGIDDGQMIKLSGQGEVGEKGAPRGDLYIVVNVKSHPLFTRDGND
                     IYFEMPITFVQATLGDEIEVPTLDGKVKYSVPEGTQTGTVFRLKEKGIPRIRGNSRGD
                     QYVKVVVEIPKKLNDKQKELLREFAKECGSNVHEKKKTFGQKIEDMFKKK"
     gene            8857..9792
                     /locus_tag="PC700_002233"
     CDS             8857..9792
                     /locus_tag="PC700_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDF2581538.1"
                     /translation="MYYIGVDIGGTGIQAGVVDNYGKIIFRSECKTVIEKGFEGILND
                     IKIMIYKLLEDNKLTMSDIKSIGFGVPGFINKEGLVTCVNLKWNKKAFNKELKRRFPD
                     AEIHGENDATVAALGEAKFGSMKGANVGVLYTLGTGVGGGIVINQKVFSGAHGLGSEI
                     GHQIIGENYFNCNCGNNGCVETFCSATAIIKYSQKLIEEGEKSRILDLAEGNLENVNA
                     KMVFDAYRENDLVAIKVINRFKDYLAKTFANTINSLDPEIISIGGGISKSSDIILDGI
                     EDLVRKFVLYKTEDIATITCATLGSDAGIIGAAFL"
     gene            9828..11201
                     /locus_tag="PC700_002234"
     CDS             9828..11201
                     /locus_tag="PC700_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2581539.1"
                     /translation="MGKTNARRTLFLIAIGSGTMLNPLNSSMISLALHSIQNDFHISF
                     TTVSWIVSAFYLASAIAQPVSGKIGDLIGRKVLFLSGLMLVILSASIVPLVQSFFILI
                     FIRVIQAIGTSTLYPSGVALIQNNIKERQSSALAVLTIFASTTAGLGPTLGGLLLDLG
                     GWHAIFLVNIPVVLVSICLGYFLFPKEVKEKKSIKETLKNVLSRLDIIGILLFSIAMI
                     FILLFLLSMKESFNLEQLVFGIILMCLFIWHELRTKLPFIDIKLFVSNPKLSKVYLQF
                     IILNFFNYILFFGLPSYFQDALHYSAKSTGLFMLFMSGIGIFISPLTGKWIDKSGTRF
                     PLVTSSIFMFISAVLLSVVFIHIPVIGKGIILSLAGISYGVGNVALQSSMFEESPRDS
                     IGTASGLFQTSRYLGSILATVLLGMVFEGNITSEQFQILGYVMVVLGTISFLLNFILK
                     KELRKVE"
     gene            complement(11389..>11891)
                     /locus_tag="PC700_002235"
     CDS             complement(11389..>11891)
                     /locus_tag="PC700_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2581540.1"
                     /translation="FIVNSKYNLDINVTTQNIIETETYQSTYIFYLGELDSKADFYFR
                     LQLCSDLLPHIIKDNAENKFLNLFDLIRYKTLDLINEDNILNKLIDFNKIKSIDEELL
                     YTGIKFINNDLNISKTSTSMFLHRNTLVYRLEKINEILGFDLKNFENAMIFYLSVKSY
                     FLYKKI"
BASE COUNT         4675 a         1296 c         2167 g         3753 t
ORIGIN      
        1 agaatatttt agaagttacg attgtatgat ttttaaacgc atagcattta ctctaggact
       61 atagactaat ataaaaaaaa actttgggaa tactaaaacc ataaatttat aaaattaata
      121 ttttatatac tgtaaaacag tgaggaggga cagtatgcat actcaactac aaaagactat
      181 tggtctttca gcggctcttt ctactgttgt aggtatggtt ataggttcgg gagttttttt
      241 taagccacaa gcaatataca ctacaactaa tggagctcca ggtctaggta ttatagcgtg
      301 gcttttaggt ggttttataa caataactgc tgggcttaca gctacagaaa tttcagctgc
      361 tataccaaaa actggtggta tgatgattta tatcgaagaa atatatggag aaaagttagg
      421 ctttttaact ggatggatgc aaactgtatt atttttccca ggtacatcag cagcattagg
      481 agtaatattt gctcaacaag catctgaact tttaggaatg agcccaaata atatggcaaa
      541 tgtacttcct atagcaattg gagttatact atttttagcg ttattaaata taataggttc
      601 atccttagga ggaaaagttc agacagtagc cactattgga aagatgatac ctttaatttt
      661 aataatagtc tttggattta ttaaaggtca aagtagtgaa gtattaaatc catttgtagg
      721 agatggtgta aatgcatcaa atgcacttgg acaagtatta atagctactt tgtttgcata
      781 tgatggttgg ataaatgttg gagctatatc aggagaaatg aagtctccag agaaagattt
      841 accaagagca atagttggtg gtttatcatt agttatggca gtttatatta taataaatat
      901 agcttatctt tgggtagtac cagcaagtga attagcaaca gttacttccc cagctacatt
      961 agtagcaaca agattgtttg gaaatatagg tggaaaagta ataactgtgg gtatattaat
     1021 atcagtattt gggacattga atggatattt attaacaggt tcaaggatac cttatacatt
     1081 agctgaaatg ggaactttac cagcatctaa aactctttta aaagtaaatt ctggaggttc
     1141 tcctgtaaat tcaatactac ttataactgt gttagcctgt gtatatgcat tatctggtca
     1201 atttaatctt ttgacagact taactatatt ttcaatatgg gtattttatg tattgacttt
     1261 tataggtgtt atgaggctta gaagacaaaa gccagattta cacagaccat ataaagtccc
     1321 tttatatcca ataataccaa ttatagctat actaggtgga ctgtttgtaa taataaatca
     1381 aatattgact tcaacaatta tatctttagg tggaatattt ataacactat taggtttacc
     1441 agtttattat tatatgaaaa aaagataact acttaattta agttagataa atttgggtaa
     1501 aggtgaaaat taacttttat ccaaattttt tatactgtta tacaatataa gcattatata
     1561 ttacttgatg tgttattttg tttgatttat aatttagtaa ttgatgcttt aagataattt
     1621 aagaaatgag gaataaaaaa tgctaggact ttatgtacat attccatttt gtgtgaaaaa
     1681 atgtaagtat tgtgatttta attcttataa aatggacata gattcaaaaa aaagatatat
     1741 agaagactta aaaatagaaa tgaaattata tagtaataaa ttatataaag ataataaata
     1801 taaaaataaa gaatgctgta gtctaaataa aaatgacaaa ataacgagta tatttgtagg
     1861 gggaggtaca cctagtatac taacatctga tgaaataaga gaagtattta taagtataaa
     1921 agaaatgttt gatatagatg agaatgcaga aataactata gagtgtaatc ctgggacatt
     1981 aacattagaa aaattaaaaa ctatgaaaga gattggcata aatagactta gtatagggct
     2041 acaggccatt caagaaaagc atttgaattt tataggaaga atacatactt atgaagaatt
     2101 tgagaaaaac tataaagatg cattaagtgt aggttttaaa aatattaatg tagatttaat
     2161 gtacagtctt ccaaatcaaa ctctatgtga ctggaaggaa actcttgaaa aagttgtaca
     2221 cttaaatcca acacatatat cagcgtattc ccttatacta gaagaaggaa ctgaactata
     2281 taatatgtat gaaagtaata aatttgagct tatagatgaa aatgtagata tagaaatgta
     2341 tgagtataca ataaactact taaagtcaaa aggatataat caatatgaaa tatcaaatta
     2401 ctcaaaagaa ggctataatt gtgagcataa tattttatat tgggaatgtg aacattatat
     2461 agggattgga gcaggagctt ctggctatat taatgagaac agatacaata atgtagaatc
     2521 tttagaagat tatcatttaa gtttggtaaa aagagaaaaa ccaatacaag aaaatgaaat
     2581 tctttctgag aaagatatga tagaagaaaa gatatttatg gggcttagaa tgaataaagg
     2641 cataaaattt gaagatttta aaaaaaaatt tggaatagat tttagagaga agtataataa
     2701 gcaaatagaa atgctattag ctagaaagct tataaatcag agctttgaag gtatacaatt
     2761 aactcaaaaa ggaagagaaa tatctaatag tgtatttata gaatttatgg aataattttt
     2821 aaggtgttga caaaataaat ttataatggt atataatata tataatcttt ttagcactcg
     2881 aacttagtga gtgctaacag ctaggaggta taataatatg gaattaaatg aaagaaaatt
     2941 aaatatcctc aaagctatag ttaaagatta tatagaaaca gctgaagcta taggttctag
     3001 aacaatatct aaaagacatg atttaggtgt tagtgctgct accataagaa atgaaatggc
     3061 tgaccttgaa gaattagggt atttaattca gcctcatact tctgcgggta gagttccatc
     3121 tgaaaaaggc tataagttat atgtaaactc tcttatgagt aaaagcgaat tggatgataa
     3181 tgataagatt cttattgaac aatgtatgaa tcataatata aatcatataa aagaattgat
     3241 tcatgaaacc tctaagctat tatcgcaact aacaaactat acaactgtag cagttactaa
     3301 gagtttaata aatcaaagtg ttataaagca catacagttg gttgctatga atgacaataa
     3361 tattttactt atagttgtaa cagataaagg ggacttaaaa aaagctaacc ttacaactaa
     3421 tgtatattta gatcaatcta agttaaattt gatttctgat aatcttacaa ggaagctttt
     3481 aggtaaaagt ataacagatt tggatgataa tctaattgct tttattaaat acgaaataag
     3541 tgagtattct ggacttatag atgagctttt aaatgctttg aactcaaata tgaaagaaga
     3601 agatttttct ctatcattaa atggagctac taacatattt agttatccag aatttaatga
     3661 tgtattaaaa gcaaagtcat ttttaaatat gttggaaaaa aaggaaacaa tagctgacat
     3721 aattaaatca aagggaatac aaaaagacaa tctaaatata ataattggta gtgataatga
     3781 ttgtgaactg gcacaggatt gtagtatagt tacagcaact tataatgtag atagagactt
     3841 ggttgggcga ataagcttta taggccctac cagaatggat tatgctagaa tttactctat
     3901 aataaattat atgagtttat taattaatag aaagtaaaga aattttaaat tgcgaggtgt
     3961 aaagtcttgg ataaaaaaaa tgagcaacag gaagtaagag aagaaaatga tacttctatt
     4021 aaccaagaat cggaaactca agtagagtta gaagaggaag ttgttaatga agaatgtgaa
     4081 acatcaagtg aaaaaactga tgaaaaagaa gttgatgatg aaaatgtaac agatataaat
     4141 tctaagttag cagaaaaaaa attgcaggat gagttggatg aattaaatga taaatatcaa
     4201 agacttcaag ccgaatatgc aaactacaga agaagaactc aacaagagaa agagacaata
     4261 ggcgtatttg caaatgaaaa aataataact gagttgatac cagttataga tagtatggaa
     4321 agagccttag atgcatgtga agataaggaa gatactatgt ataagggtat aagccttgta
     4381 cataaacaat taatagacac tttggtaaaa tttggagtag aagaaataga agcagaatct
     4441 aaggaatttg accctaatct acatctagca gttatgcaag aaagtgtaga tggagttgaa
     4501 gcaaatcaaa tagtaatggt acttcaaaaa ggatataagc taggaactaa agttgtaaga
     4561 ccttcaatgg ttaaagtttc ttgctaagta taaattaaag gttaaattat ttaacctaat
     4621 ataaaatatt aaaataagta aaacttaatt atataattac tcgaggagga ttttagtcat
     4681 gggaaaaata ataggaatag atttaggaac aacaaattca tgtgttgcag tgttagaagg
     4741 tggagaagca caaataatag caaatagtga aggtatgaga actactccat cagtagtagc
     4801 atttacaaaa gatggtgaga gaatagttgg agaacctgca aaaagacaag cagttacaaa
     4861 tgcagataaa acaataactt ctataaaaac tcatatggga actgattata aagtaaatat
     4921 agatggaaaa tcatatactc cacaagaaat atcagcaata atattacaaa aattaaaatc
     4981 agatgcagaa agttacttag gacaaacagt aacagaagca gttataacag taccagctta
     5041 ctttacagat gctcaaagac aagcgactaa agatgctggt agaatagcag ggcttgatgt
     5101 taagagaata ataaatgagc caacagcagc agctcttgct tatggtatgg ataaattaga
     5161 ccaagaaaag aaaatattag tatttgactt aggtggagga acttttgacg tatctatact
     5221 tgaaatagga gatggaactt ttgaagtttt agctacagct ggtaacaata gattaggtgg
     5281 agatgacttt gaccaaatag taatagatta tttagcagat gaatttaaaa aagctgaagg
     5341 tgtagattta agaaatgata aaatggctct tcaaagatta aaagaagcag cagaaaaggc
     5401 taagaaagaa ttatcatcaa caatgagttc aaacataaac ttacctttca taactgctac
     5461 agcagaagga ccaaaacact taaatataga tttatcaaga gctaaatttg aagaattaac
     5521 tagaggttta gttgaaaaaa ctatggaacc aactaagaca gctttacaag atgcaggact
     5581 ttctacaggt gatatagatg atgtattatt agtaggtggt tctacaagaa taccagcagt
     5641 acaagaagct gttaagaagt ttataggaaa agaacctcat aaaggtataa acccagatga
     5701 gtgtgttgca gcaggtgctt ctatacaagc tggagtttta gcaggggatg ttaaagattt
     5761 attattacta gatgttactc cattatctct tggtatagaa actatgggta atgtaatgac
     5821 taagataata gaaagaaata ctacaatacc aactaagaag tctcaaatat tctcaactgc
     5881 tgctgataac caaacagctg ttgatataca tgttcttcaa ggtgaaagaa gtatggctta
     5941 tgacaataca actttaggaa gattccaatt aacagatata ccaccagcac aaagaggaat
     6001 acctcaaata gaagttactt ttgatataga tgcaaatggt atagttaatg tttcagctaa
     6061 agatttaggt acaggaaaag agcaaaaaat aactataaca tcaaacacta atttatcaga
     6121 agcagaaata gagcaaaaga taaaagaagc agaaatgaat gcagaagctg acaaacaaaa
     6181 gaaagaaaaa atagaagcat tcaatcaagc tgaatctact atatatcaaa ctgaaaaaac
     6241 tttaaatgaa cttggagata aaataagttc aggtgaaaaa gaggatatag aaaaagctat
     6301 agcagactta aaagctgtaa aagataatca agatgctaca gcagaagaac ttaagaaagc
     6361 tacagatgaa gtaatgacta agttccaaaa agtatctcaa gaaatgtacc aaaaagcagc
     6421 tcaagaacaa caagcggcac aaggtgcaga acaagcacaa gacaatggac ctaaagatga
     6481 caatgtagta gatgctgact ttaaagaagt agatgaagat aaataagcta tataatatgt
     6541 aacactaaat gataattgga acatatatga tttgtgaaaa tcatatagat gttaaagata
     6601 tatggacaat acacatcata aagaatataa tatatcatag attatataac ttaaagtaga
     6661 atataagaat taatgatagc ttgtagttta ttattctaca agctatcgtt ttgtaaaatg
     6721 aaggtggtgt caatttttga gtacaaaaag ggattattat gaagtcttag gtatcagtaa
     6781 agatgcagaa gctcaagaga taaaaaaagc ttatagaaaa ttagcaatga agtaccatcc
     6841 agatagaaat cctggagata aagaggcgga agaaaaattt aaagagataa atgaagctta
     6901 tgaagttcta tctgatgata caaagagaaa aacttatgac cagtttggac atgatggatt
     6961 aaatggtcaa ggtggttttg gtggtcaagg aggatttggc ggtcaaggct ttggtggttt
     7021 tgaagatatg ttcggagata tatttggtga catgtttgga ggaagcttcg gaggtggaag
     7081 agcaagaaga agagggcctc aacgtggtgc agatataaga cagagtgtta ctatatcttt
     7141 tgaagaagct gcatttggta aaaagatgtc tataaaagta aatagaagtg aagagtgtga
     7201 agaatgtaat ggaactggag caaaaccagg aacatcaaag aaaacttgtt caacttgtaa
     7261 tggaactggc caagttagaa ctgttcaaag aactccattt ggtaatatag caagttcaag
     7321 accatgtagt gcctgtaatg gaactggaga agtgattgaa tctccatgta gtaaatgtca
     7381 tggaactgga aatacaagaa aagttaagac tatagaagta gatatacctg ctggtataga
     7441 tgatggtcaa atgataaaac tttctggtca aggtgaagta ggagaaaaag gtgctccaag
     7501 aggagactta tatattgtag ttaatgttaa gtcacatcca ctatttacta gagatggaaa
     7561 tgatatttat tttgaaatgc caattacatt tgttcaagca actttaggtg atgagataga
     7621 agtacctact ttggatggaa aagttaaata tagtgtgcca gaggggacac aaactggaac
     7681 tgtatttaga cttaaagaga aaggaatccc tagaattaga ggtaattcaa gaggagacca
     7741 atatgttaag gttgtagttg aaattcctaa aaaattaaac gataagcaaa aagaactatt
     7801 gagagaattt gcgaaggagt gtgggtcgaa cgtccacgaa aagaagaaaa ccttcggaca
     7861 aaagatagaa gatatgttta agaaaaagta gttaaagtag ccaatatata atcatatttt
     7921 tgaaatctta gttatttata agattgtcaa aggagatatt atgattttta tattggctat
     7981 ttttgtataa aaaataaatg tgcataaaat aaaactgaat ataaataatt ttctgaaaaa
     8041 tacttgtcct aaaatatata atgtgctata atataatcaa ataaaagaaa ggggtgattc
     8101 cagtgggaac ggatgtggca ctgccaaatg atgaagaaaa ctttattgta atatacaaaa
     8161 taaaattaga tacttttagt gctagattaa gtactggaaa aactcatgag ctgggataat
     8221 tattgtaatt acctagactc aaacttttag tagtaaaata gtaatattat atagacaagg
     8281 tatctaattt ttaaatcttt tgtatgagtt aaaattttta taaagggtat atctgattag
     8341 aatgtaatta gggaaaccta gttgtgaatt acttttagta tatacatgtg taaagaaata
     8401 tgtatatggt attggttatt gtgctagatg tgtatgtaaa ggaatatgtg tctagttggg
     8461 tatatgtaaa gaaatatact tataatctat gaaaaatttt tagtataaat tgctaaaaca
     8521 ttgagattgt tttttataga aagttagttt ataccaacta aaaatgtgct taagatttgt
     8581 ttgatgttta attataaata aggttgtaga taaaaaaata agtaaagaca aaggtttaaa
     8641 atacttgctt taaatatagt ttgttttgaa cacttgccaa attaaaataa ttaataaaat
     8701 agacatcttc tcatagtaaa atatactgtg agaggatgtt tttgctttgt tcataaactt
     8761 ttatgaaaaa tttgcatagt gtcacatttt aatatactat atatataata aaatatagat
     8821 aaaattaaat gattcaaaag agaaaggtga aaagagatgt attatatagg tgtagacata
     8881 ggaggaacag gtatacaagc aggagtagtt gataactatg ggaagataat ttttaggtca
     8941 gagtgtaaaa cagtaattga aaaaggcttt gaaggaatac ttaatgacat aaaaataatg
     9001 atttataagt tgttggaaga taataaactt actatgagtg acataaaatc tattggattt
     9061 ggtgttccag gttttataaa taaagaagga ttagtaactt gtgtaaattt aaaatggaat
     9121 aaaaaagctt ttaataaaga gttaaaaaga agatttccag atgctgaaat tcatggagaa
     9181 aatgatgcaa cagtagcagc acttggagaa gcaaagtttg gaagtatgaa aggtgctaat
     9241 gttggagtat tatatacttt aggaactggt gtaggtggag gaatagtaat taatcaaaaa
     9301 gtcttttctg gagcacatgg attgggttca gaaataggtc atcaaattat aggagaaaac
     9361 tattttaatt gtaattgtgg aaataatggt tgtgtagaaa ctttttgttc agcaactgca
     9421 attataaagt attctcaaaa actgatagaa gaaggagaaa agagtaggat tttagatttg
     9481 gcagagggaa atttagaaaa tgtaaatgca aagatggtgt ttgatgcata tagagaaaat
     9541 gatttagtgg caattaaagt aataaataga tttaaagatt atttagcaaa aacttttgca
     9601 aacacaataa attctttaga cccagaaatt atatctatag gaggaggcat ttctaaatct
     9661 agcgatataa tacttgatgg aatagaagat ttggtaagaa aatttgtatt atacaaaaca
     9721 gaagatatag ctacaattac atgtgcaact ttaggttcag acgcaggaat tataggagca
     9781 gcttttttat aatgtaaatg aactgcttat tggaggcgtg atgatatatg ggtaaaacaa
     9841 atgctagaag aacgcttttt ttgatagcaa taggttcagg aactatgctg aatcctctta
     9901 attcttctat gatttcactt gctttacata gtatacaaaa tgattttcat attagtttta
     9961 caactgtatc ttggattgtt tcagcatttt acctagctag tgctattgca caaccagttt
    10021 caggtaagat aggagattta ataggtagaa aggtcttatt tttgagtggt ttgatgcttg
    10081 taattttatc agcatccata gtaccacttg ttcaatcatt ctttatattg atttttataa
    10141 gagtaattca agcaattgga acaagtaccc tatatccatc tggagttgct cttatacaaa
    10201 ataatataaa agaaagacaa tcttctgcat tagcagttct aaccattttt gcttcaacta
    10261 cagcaggatt aggtccgaca cttggaggat tattgttgga cttaggagga tggcatgcta
    10321 tatttcttgt aaatatacct gttgttttgg tttctatatg cttagggtat tttttattcc
    10381 caaaagaagt aaaagaaaag aaatctataa aagaaacttt aaaaaatgtt ttgtcaagat
    10441 tagatattat tggcatactt ctattttcaa ttgcaatgat ttttatatta ctatttttgc
    10501 tatctatgaa ggaatctttt aacttagaac aacttgtatt tggtataata ctgatgtgtc
    10561 tttttatttg gcatgaattg agaacaaaat tgccatttat agatattaag ctttttgtaa
    10621 gtaatcctaa attgtcaaaa gtttacttgc aatttattat attaaatttc tttaattata
    10681 ttttgttttt tggtcttcct agttattttc aagatgcctt acattatagt gcaaagtcga
    10741 ctggattatt tatgctattt atgtctggta taggtatatt tatatctcca ttaactggaa
    10801 aatggataga taaatctgga actagatttc ctcttgttac atcatcaatc tttatgttta
    10861 ttagtgcagt acttctttca gtagttttta tccatatacc tgtaatagga aagggtatta
    10921 tattatcatt agcaggtatt agttatggag ttggtaatgt agccctgcaa tcctctatgt
    10981 ttgaagaaag tcctcgtgac agtataggga cagcttctgg tctgtttcaa actagtcgtt
    11041 atttaggttc tattttagca actgttttac ttggaatggt atttgaagga aatataacat
    11101 cagaacaatt tcagatatta ggttatgtta tggtggtctt aggtacaata agttttttat
    11161 taaattttat attaaagaaa gaattaagaa aagtagaata gtggatttat ggatttaata
    11221 agtctaataa ctgataagtt taaaaatatt gtttgtaaaa cataactggt ttgatattta
    11281 ttaattctaa gttttgaagc atagagtact cgataagtat acaatatttt gaattaaaat
    11341 taagcagaaa tttgaactag tctttcaaat ctctgcttaa tttagtagtt aaattttttt
    11401 atagagaaaa taagatttga cacttagata aaaaatcatt gcattttcaa aattctttaa
    11461 atcaaaccca agtatctcat taatcttttc caatctatat actagcgtgt ttctatgtaa
    11521 aaacatacta gtagatgtct tagaaatatt taaatcatta tttataaatt ttattcctgt
    11581 atatagaagt tcttcgtcaa tagatttaat cttattaaaa tctattaatt tattgagtat
    11641 attatcctca ttaattaaat ctaaagtttt atatctaata aggtcaaaca aatttaaaaa
    11701 cttattttca gcattgtctt ttataatatg aggtagtaaa tcagaacata actgtaatct
    11761 aaaataaaaa tcagctttac tatctaattc accaagataa aaaatataag tactttgata
    11821 agtttcagtt tcaattatat tttgtgtagt tacattaata tctaaattat atttagaatt
    11881 tactataaaa a
//
LOCUS       DAMJWP010000101        11870 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.105, whole genome shotgun sequence.
ACCESSION   DAMJWP010000101 DAMJWP010000000
VERSION     DAMJWP010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11870
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.105"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            85..1002
                     /locus_tag="PC700_002236"
     CDS             85..1002
                     /locus_tag="PC700_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDF2581541.1"
                     /translation="MMILIQSRGKMKCKELSEELEVSERQIKSYKMYLEQAGIFINST
                     PGIYGGYEIDKCNSISLIKLLDSEVSILDMINSQLEYNNDIYKNEFNNIVEKIKAVLN
                     TGEKSDTYMDYFTVQAQRNCDYKSEKNKCNEIIRAYTTKHKFWIEYYSLNSGNSERIV
                     HPYGLFNYKSDTYMVAFCEKRFKFIDFKLCRIKDYKVLEEKYNVDKSFSWDEYSKNSI
                     GIYKGEEINVVIKISHPFSIIIKEKVWVNNQQIIEYDDKSIMFKAKMRGYEEIKSWIL
                     SMGAYVEVVEPDRLRNDILSEIEKMKKIY"
     gene            1080..1817
                     /gene="cas6"
                     /locus_tag="PC700_002237"
     CDS             1080..1817
                     /gene="cas6"
                     /locus_tag="PC700_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HDF2581542.1"
                     /translation="MKMKIEFSTGCIPISYNSLFMSIIKEAIRKSNEDYYKNMYYYKE
                     KNNKKTKNFTFSVYIKKYSIEGDNFIIEDKVILNISTPDLELGFHIYNGLMTSKKCLY
                     KAYELTRIRIDLSREKKVTEERVLFNALSPICVKSKEGKFLEITDDRYIEELNYITNE
                     VVKNYRGNGLKRKLEFENIGLKKIVVKESLREFKKITGKEYQYINGYKGKFILKGDID
                     DLNLIYNLGIGFRRAQGFGDVDILEWR"
     gene            1823..3700
                     /gene="cas8a1"
                     /locus_tag="PC700_002238"
     CDS             1823..3700
                     /gene="cas8a1"
                     /locus_tag="PC700_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas8b1/Cst1"
                     /protein_id="HDF2581543.1"
                     /translation="MKLRVYRGDWFFNMGIVGFLNIIKKADKQAEIFIMEDYIEFDSL
                     FLENFHEYYFNYFMDEYDVSKRIKKNIDYSINFIKSKPDRIKDGIKKIKDSVKQQNDK
                     IKKFDEEKFNLIKEKLDSMSKIKSYDEFDSLEALVDETIDIFKIKSINDRLTANLYKY
                     VVQDNYFGQVSFFNVAKAKLDLDGLKQIMFNDYLRQIIYFGELADLLEENDYDKLKNY
                     LNDRLNSIAKDINEKKVSKSSINTIEKIMKEINKNFIKKNKSIEDIKEYMDSLEVCEM
                     CGLYKGILDEYSESNFAPLGVSTNNARNMFWNQAYVPSICDICKLILFCTPAGATYTR
                     KNYLINEENEFYLFVNMDTSINELFERNNSLKAQKSEYSDSKDENPFNQLIKSIVEEN
                     TLKSEWQLRNILFVEFKASIDSKKCKINYFNIPTYLAKFFTDKYANKKIQSIYDYKLK
                     SNVLDLLLKNRDLKHLTNNILRNKVKNDMESNNKSNISGIDCFRVVQLRALINSYKKG
                     VYKMDSKNLEKNDEKLRIIYYLGCDIHDYFVNKNSKNKIDGVSYKLLNSVKVGNKSDF
                     MDTIIRVFMSAEKQIPAFILDIEIEKDLDFESIGHAFISGLISGKYEGKDKLPNEKEE
                     K"
     gene            3702..4616
                     /gene="cas7i"
                     /locus_tag="PC700_002239"
     CDS             3702..4616
                     /gene="cas7i"
                     /locus_tag="PC700_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HDF2581544.1"
                     /translation="MKRGLTFTVIAQAQSLNYGEGIGNISELKKLSRDDGNIYTFASR
                     QCLRYDIVRLAAELFNWNLQVVDKEKGTIQFKDNISIRESEEMDLFGYMKTNKKDEKD
                     KKGGSLTREATVRLSNAISLEPYRSDMDFLNNKGLADRIGEHPNLANIEQHLSYYTYT
                     VTIDLSKIGKDGDIELDNKEKCRRVVEFLEIIKVLNRNIRGRQENLSPLFVVGGVYEI
                     ANPFFLGRIKLKGDKNGFKINKQAIEEVIQGTFLGKDLKEFTYVGMVDGVFINKEEFK
                     GLFEDNFLSVDKFFGQLVKEVKEYYGVN"
     gene            4618..5319
                     /gene="cas5b"
                     /locus_tag="PC700_002240"
     CDS             4618..5319
                     /gene="cas5b"
                     /locus_tag="PC700_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas5"
                     /protein_id="HDF2581545.1"
                     /translation="MKVLKLDLFQETACYKKPFAMKITETYPLPPYSTINGLIHKILN
                     ATEYIPFNISVQGTYESIFNNYQTTYFYKKDSITSMPMNSHMLLNVNLIIHIGGDFDL
                     LEEIYDSLLNYDEHLSLGRKEDLVRINDIRFVEVNKFEVDEDIQLDDYENYKLSECNI
                     RMPIYIPKTSLQDTDIDGISYRLNNYYDNNAVEDKKRVWNKVDSYYVEDGNTISIGDI
                     LLDNEGDLLYFNNIN"
     gene            5330..7651
                     /locus_tag="PC700_002241"
     CDS             5330..7651
                     /locus_tag="PC700_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HDF2581546.1"
                     /translation="MLYAKSNPVETLREHTDELLKQMNVLRESYGKNINSLDFLEEEI
                     FWQLLDFVIEFHDIGKAFSPFQELIKSRMDTKINEPKIVTHLENNVGHNYLSPAFIDY
                     SYIDRKKNKELRAVLNQVIVYHHERDIFIDKDFKNLIQKILDEDLINKVYELQREFKV
                     RYPIKTEKLSKVYLQSVEKRIDKNHKYYNLYIMLKGILHRLDHSASAHEVVECNNVIN
                     IGEQTENYLMKEFGSLREAQSFAKSNRNKNIILIASTGMGKTETALIWIDKDKAFFTL
                     PLRVSINALFDRAKNIIGVGEANDTFLGLLHSTAIDYLEESNQENSSEIVDLAKLLSC
                     KLTFSTIDQIFKFPFLYRGYEKVYSTLAYSKVVIDEIQAYSPEIAAVLVKGIEMIHKI
                     GGRFMIMTATMPTIYIDELKKRGVMNSNLADLTCNTEKIRHCVSIVENSIDENLGKII
                     QSGMNKKVLVIVNTVKSAVEKYELIEEIVKPKGIDVNLNLLHSMYIQEDRAKLEKYIK
                     EFADSDSNGIWITTQLVEASLDIDFDELHTENSTLDSLFQRFGRCYRSREYEENSPNI
                     FIYTEKATGIGSIYDKDIVEKGLELLKTFINGKECVKMKEKYKVEMVKILYSKESLEG
                     TAFEKRFTSALNILDTITPYSLGSKDAQNILRNIEGYTVIPEDIYNKIEETLIKDYEE
                     LGQELINAYSNDDRQLINKIKSKRKKARREIIKKTVNLPIYKAKQNVIDIQVKGLEDL
                     KILLYKYDIHENKHTKQIFGKGVLISNEIDQFI"
     gene            7666..8217
                     /gene="cas4"
                     /locus_tag="PC700_002242"
     CDS             7666..8217
                     /gene="cas4"
                     /locus_tag="PC700_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HDF2581547.1"
                     /translation="MWLKFTKEGDFVPIDLEVLKVQGVKINYYYVCKRKLWLFSKGIT
                     MEQNSSRVLSGKIVHEDSYKRMKNKEMLIDDILKLDIIKDEYVKEVKISSKMKEADEM
                     QLYYYLYYLKNSMGIEKKGTINYVKEKKQEELILTEEKEKIIENTLININEINMQLSP
                     PKIEKKHYCTKCAYYEFCFVKEE"
     gene            8220..9200
                     /gene="cas1b"
                     /locus_tag="PC700_002243"
     CDS             8220..9200
                     /gene="cas1b"
                     /locus_tag="PC700_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1"
                     /protein_id="HDF2581548.1"
                     /translation="MYKDYYIISNGNIKRKENTIYYYNLEGEKKILPVEQVDDIHIYG
                     EVDLNTKLINYISKYGVMLHFYNYYGYYSGTYYPRKKNVSGFSLVCQSACYLDKNERL
                     YLAKSFVESASYHILRNMRYYKVDESLIDTIKDELNDLNDVKEISELMGKEGRIRKTY
                     YKSFNQILKDDFEFYKREKRPPKDPVNTLMSFGNSIMYTNVLSEIYKTQLDPTISFLH
                     EPSTKRFSLSLDIAEIFKPIIVDTIIFSLINNKRINKNDFEYQDKICYLSEKGKKKFL
                     QEFENKMQTTIKHRNLNRKVSYRMFIRLECYKLIKHFINDERYKVLKVWW"
     gene            9203..9469
                     /gene="cas2"
                     /locus_tag="PC700_002244"
     CDS             9203..9469
                     /gene="cas2"
                     /locus_tag="PC700_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HDF2581549.1"
                     /translation="MFVIVTYDIVEARSLNRIRRILRKYLTWTQNSVFEGNITDGKLH
                     KCISEIENIIDNSEDSIYVYEIKNPNSIKKKCYGIDKYSDEMFI"
     repeat_region   9653..11270
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=9653..9681
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaat"
     gene            11460..>11870
                     /locus_tag="PC700_002245"
     CDS             11460..>11870
                     /locus_tag="PC700_002245"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDF2581550.1"
                     /translation="MIKKLNNKDINKIMEIWEKSTIKAHDFISKEYWQNNYNTVKNEY
                     IPISDTFVYDDGDEIKGFISIIDKSFIGALFIEPKYQNLGIGGKLLDYATKKYKSLSL
                     AVYKDNKKAVVFYNKKGFNIVKEQVNEDSGFKEYI"
BASE COUNT         5021 a         1024 c         1995 g         3830 t
ORIGIN      
        1 atatgtgtag aaataaataa taaaaatatc aattaatgtt attagggggg gttactcatt
       61 agtaaattag ctaatacact tagaatgatg atattaattc aaagcagagg caaaatgaaa
      121 tgcaaagaat tatcagaaga gttggaagta agtgaaaggc aaataaaaag ttacaagatg
      181 tatttagagc aagctggtat atttataaat tcaacacctg ggatttatgg tggttatgaa
      241 atagataaat gtaatagcat atcattaatt aaattattag atagtgaagt ttcaattcta
      301 gatatgataa attcacagtt agagtataat aatgatattt acaaaaatga atttaataat
      361 attgtagaaa aaataaaagc agtattaaat acaggagaaa aaagcgatac atatatggat
      421 tattttacag ttcaagcaca acgtaattgt gattataagt ctgaaaaaaa taagtgtaat
      481 gaaataatta gagcatatac cacaaaacac aaattttgga tagaatatta ttcattaaat
      541 tcaggaaata gtgaaagaat tgttcatcca tatggtttat ttaattataa atcagatact
      601 tatatggtag ctttttgtga aaaaagattt aagtttatag actttaaatt atgtagaatt
      661 aaagattata aggttttaga agaaaaatat aatgtagata aaagttttag ttgggatgaa
      721 tatagtaaaa atagtatagg aatttataaa ggagaagaaa ttaatgttgt tataaaaatt
      781 agtcatccat tttcaataat tataaaggaa aaagtttggg ttaataatca acaaatcata
      841 gaatatgatg ataaatctat aatgtttaaa gctaaaatga gaggttatga ggagataaaa
      901 agttggattt taagtatggg agcttatgtt gaggttgtag aaccagatag acttagaaat
      961 gatatattgt cagagattga aaaaatgaaa aaaatttact gattttttca aataatttat
     1021 acttggacca atatacttca cggtctagtg ttaatatatt aattgagaga ggtgatatta
     1081 tgaaaatgaa aattgaattt agtacaggtt gtataccaat atcttataat tcgctcttta
     1141 tgagtattat aaaagaagca ataagaaaat ctaatgaaga ttactataaa aacatgtact
     1201 attataaaga aaagaataat aaaaaaacta aaaattttac attttcggtt tatataaaaa
     1261 agtatagtat tgaaggtgac aattttatta tagaagataa agtaatatta aatataagta
     1321 ctccagattt agaattgggt tttcatatat acaatgggtt aatgacttct aaaaaatgtt
     1381 tatataaagc ctatgaactt acaagaatta gaatcgattt atcaagagaa aagaaggtaa
     1441 cagaagaaag ggtactattt aatgcattgt ctccaatctg tgtaaagtct aaagaaggaa
     1501 aattcttgga gataactgat gatagataca tagaagaact aaactatata actaatgaag
     1561 tggttaaaaa ttatagggga aatggattaa aaagaaagtt agaatttgaa aatataggtc
     1621 tcaaaaagat agtagttaaa gagagcttaa gagaatttaa aaaaataaca ggtaaagagt
     1681 atcaatatat taatggttac aaaggtaaat ttatattgaa aggtgatatt gatgatttaa
     1741 atcttatcta caatctagga attggtttta gacgtgctca gggatttgga gatgtagata
     1801 ttttagaatg gaggtgagat aaatgaagtt aagagtttat agaggagatt ggttttttaa
     1861 tatgggtata gttggatttt taaatataat aaagaaagca gataaacaag ctgaaatatt
     1921 tattatggaa gattatatcg aatttgatag cttatttctt gaaaactttc atgagtatta
     1981 ttttaattac tttatggatg aatatgatgt atcaaaaaga attaaaaaaa atattgatta
     2041 cagtataaat tttattaaat ctaagcctga tagaatcaaa gatggaatta agaaaattaa
     2101 agatagtgtg aaacaacaaa atgataagat taaaaaattt gatgaagaaa aatttaacct
     2161 gataaaagag aagctagata gcatgtctaa gataaaaagt tatgatgaat ttgacagttt
     2221 agaagcttta gtagatgaga ctatagatat atttaaaata aaatcaataa atgacaggct
     2281 tacagcaaat ctttataaat atgtagttca agataattat tttggacaag ttagtttttt
     2341 taatgttgct aaggcgaaat tagatttaga tggattaaaa cagataatgt ttaatgatta
     2401 tttaagacaa attatatatt ttggagagtt agcagattta ttagaagaga atgactatga
     2461 taagttgaaa aactatttaa atgatagatt aaatagtata gctaaggata taaatgaaaa
     2521 aaaagttagt aagtctagta taaatactat agaaaaaatt atgaaggaaa taaataaaaa
     2581 ctttataaag aaaaataaaa gcatagaaga tataaaagag tatatggata gtttagaagt
     2641 atgtgaaatg tgtggtttat ataagggaat attagatgaa tactcagaaa gtaattttgc
     2701 tccacttgga gtaagcacca ataatgctag gaatatgttt tggaatcagg cgtatgtacc
     2761 atcaatatgt gatatttgta agctaatatt gttctgtact ccagcagggg ctacttatac
     2821 aagaaagaat tatttaatta atgaagaaaa tgagttttat ttatttgtta acatggatac
     2881 ttctataaat gagctttttg aaagaaataa tagtttaaaa gctcaaaaaa gtgaatattc
     2941 agattccaaa gatgaaaatc cttttaatca gcttataaaa agtatagtag aagaaaatac
     3001 attaaaaagt gaatggcaac ttcgtaatat actttttgtg gaatttaaag caagtataga
     3061 ttctaaaaaa tgtaaaataa attattttaa tattccaact tatttagcaa aattttttac
     3121 agacaagtat gcaaataaaa aaatacaaag tatctatgat tacaagctta aatcaaatgt
     3181 actagattta ctcttgaaaa atagggactt gaagcatcta actaataaca ttttaagaaa
     3241 taaagttaaa aatgatatgg aaagcaacaa caaatcaaat atatcaggaa tagattgttt
     3301 tagagtagtc caattaagag cattaataaa tagctacaaa aagggggtat ataaaatgga
     3361 tagtaaaaat ttggagaaga atgatgaaaa gctgagaata atttattatt taggttgtga
     3421 tatacatgac tattttgtta ataaaaattc taaaaataaa atagatggag taagctataa
     3481 acttttaaat tcagttaaag tcggaaataa aagtgatttt atggatacaa ttataagagt
     3541 ttttatgtca gctgaaaaac agattccagc atttatttta gatatagaaa ttgaaaaaga
     3601 tttagacttt gaatctatag gtcatgcttt tatatctgga ttaatttctg gaaaatatga
     3661 aggaaaagac aaactaccaa acgaaaagga ggaaaaataa gatgaaaaga gggttaacat
     3721 ttacagtaat agcacaggct caaagtttaa actatgggga agggataggt aatatatcag
     3781 aattaaaaaa attaagtaga gatgatggaa atatatatac atttgcatct cgccagtgtt
     3841 taagatatga catagtacga ttagcagcag aattgtttaa ttggaattta caggttgtcg
     3901 ataaagaaaa aggtactatt caatttaaag acaatataag tataagagaa tcagaagaaa
     3961 tggatttatt tggatatatg aaaacaaata aaaaagatga gaaagataaa aaaggaggtt
     4021 ctttgactag agaagctact gtaagattaa gtaatgcaat ttcccttgag ccatatagaa
     4081 gtgacatgga ctttttaaat aataaaggat tagcagatag aataggagaa catcctaatc
     4141 ttgctaacat agagcaacat ttaagttatt atacatatac agtgactata gatttatcta
     4201 aaataggtaa ggatggagat atagaacttg ataataaaga gaaatgtaga agagtagtag
     4261 aatttttaga aataataaaa gtattgaata gaaatattag aggtagacaa gaaaatttat
     4321 ctcctctatt tgtagtagga ggagtatatg aaatagcaaa tccatttttc ttgggtagaa
     4381 ttaaattaaa aggagataaa aatggattta aaataaataa gcaagctata gaagaagtaa
     4441 ttcaaggtac ttttttagga aaagatttaa aagaatttac ctatgttgga atggtagatg
     4501 gggtatttat aaataaagaa gaatttaagg gattgtttga agataatttt ttaagtgtag
     4561 ataaattttt tggacaacta gttaaagaag taaaggaata ttatggggtg aattaatatg
     4621 aaggttttga aattagattt atttcaagaa actgcatgct ataaaaaacc atttgctatg
     4681 aagattacag agacatatcc actgcctcca tattctacaa taaatggatt aattcacaaa
     4741 atattaaatg ctacagaata tataccattt aatataagtg tacagggaac atatgaaagt
     4801 atatttaata attaccagac aacatatttt tataaaaaag atagtattac atctatgcca
     4861 atgaatagtc atatgctatt gaatgtaaat ttgataattc atataggtgg ggattttgat
     4921 ttattggaag aaatatatga tagtttatta aattatgacg agcacttatc attaggtaga
     4981 aaagaagatt tagtaagaat taatgatatt agatttgtag aagtcaataa atttgaagta
     5041 gatgaggata tacaattaga tgattatgaa aattataagt tatcagaatg taatataaga
     5101 atgcctatat atataccaaa aacaagttta caagatacag atatagatgg tataagttac
     5161 agactcaata attactatga taataatgct gtggaagata aaaaaagggt ttggaataag
     5221 gtagatagtt attatgttga ggatggaaat actatatcaa ttggagatat attattagat
     5281 aatgaaggag atttattata ttttaataat ataaattagg agggagagta tgttatatgc
     5341 taaatctaat ccagtggaaa cacttagaga acatactgat gaacttttaa aacaaatgaa
     5401 tgtattaaga gaaagttatg gaaaaaatat aaatagctta gattttctag aggaagagat
     5461 attttggcaa ttacttgact ttgttataga atttcatgat attggaaaag cattttcacc
     5521 gtttcaagag ttaataaaaa gcagaatgga tactaaaata aatgaaccta aaatagtaac
     5581 tcatttagaa aataatgtag gccataatta tctttctcct gcatttattg attatagtta
     5641 tatagacagg aaaaaaaata aagaacttag agcagtatta aatcaagtta ttgtatatca
     5701 tcatgaaaga gatatcttta tagataaaga ttttaaaaat ttaatacaaa aaatattaga
     5761 tgaagattta ataaataaag tttatgaact acagcgtgaa tttaaagtta gatatccaat
     5821 aaaaacagaa aaattgagta aagtatatct acaaagtgta gaaaaaagaa tagataaaaa
     5881 tcacaagtac tataatttat atattatgtt aaaaggaata ctacatagac tagaccatag
     5941 tgcttctgct catgaagttg tggaatgcaa taatgttata aatataggag aacagacaga
     6001 aaattattta atgaaagagt ttggtagttt gagagaggct caatcatttg ctaaatctaa
     6061 tagaaataag aatataatac tgattgcatc tacaggaatg ggaaaaacag aaactgcttt
     6121 gatttggata gataaggata aagcattttt tacactacca ttaagagtaa gtataaatgc
     6181 attatttgat agggctaaaa atataattgg agtaggagaa gcaaatgata cattcttagg
     6241 tctgttacat tcaacagcaa tagattattt agaagaaagt aatcaggaaa attcaagtga
     6301 gattgtagat ttagcaaagt tattatcttg taaactgaca ttttccacaa tagaccaaat
     6361 atttaaattt ccttttttgt atagaggcta tgaaaaagtg tactcaacat tagcatattc
     6421 aaaagtagta atagatgaga tacaagcata ttcaccagaa atagcagctg tattggtaaa
     6481 aggtatagaa atgatacata agataggtgg cagatttatg ataatgacag ctactatgcc
     6541 aaccatatat atagatgaat taaaaaaaag aggtgtaatg aatagtaact tagctgactt
     6601 gacatgtaac acagaaaaaa taagacattg tgtgtctatt gtagaaaatt caatagatga
     6661 aaacttaggt aaaataatac aaagtggaat gaataaaaaa gttcttgtaa tagtaaatac
     6721 agttaaaagt gctgtagaaa aatatgagtt aatagaagaa atagttaaac caaaaggaat
     6781 agatgtcaac ttaaatcttt tacattctat gtatatacaa gaagatagag caaaattaga
     6841 aaaatatata aaagaatttg cagatagtga tagtaatggt atttggatta ctacacaatt
     6901 agtggaagca tcccttgata ttgattttga tgagcttcat acagaaaatt caacacttga
     6961 cagtctattt caacgatttg gtagatgtta tagaagtcgt gagtatgaag aaaatagccc
     7021 aaatatattt atatatacag aaaaagctac aggaataggt agtatttatg ataaggatat
     7081 tgtagaaaaa ggacttgagc tactgaaaac ttttataaat ggaaaagaat gtgtgaagat
     7141 gaaagaaaaa tataaggtag aaatggttaa gatactatac tctaaagaaa gtctagaagg
     7201 tacagctttt gaaaagagat ttaccagtgc tttaaatatt ttagatacta taacgccata
     7261 tagtttaggt tctaaagatg cacaaaatat acttagaaat attgaaggat atacagtaat
     7321 tccagaggat atatataata aaattgaaga aactttaatc aaagattatg aagagttggg
     7381 acaagagctt ataaatgcat atagtaatga tgatagacaa ttgataaata aaataaagtc
     7441 taagagaaaa aaagcaagaa gagagataat taaaaaaact gtaaatttac caatatataa
     7501 agctaaacaa aatgtaattg acatacaggt gaaaggtctt gaagatttaa aaatactttt
     7561 atataaatat gatatacacg aaaataagca tacaaaacag atatttggta aaggtgtgct
     7621 tataagtaat gaaatagacc aatttatttg atttttatat taacaatgtg gctaaaattt
     7681 actaaagaag gtgattttgt gccaatagat ttagaagtat taaaagttca aggcgtaaaa
     7741 ataaattatt attatgtatg taaaagaaaa ctatggctat tttctaaagg aataactatg
     7801 gaacaaaata gtagtagagt attgtctgga aagatagtac atgaagattc atataaaaga
     7861 atgaaaaata aagaaatgtt gattgatgat atcttgaagc tagatataat aaaggacgag
     7921 tatgtaaaag aagtaaaaat aagttcaaag atgaaagaag cagatgaaat gcagttatac
     7981 tattacttat actatttaaa gaatagtatg ggtatagaga aaaaaggaac tataaattat
     8041 gttaaggaaa agaaacaaga agaattgata ttgacagaag aaaaagaaaa aattattgaa
     8101 aacacactta taaatataaa tgaaataaat atgcagttat ctcctcccaa aattgagaaa
     8161 aaacattatt gtacaaaatg tgcttattat gaattttgtt ttgtgaagga ggagtaacaa
     8221 tgtataaaga ctattatatt ataagtaatg gaaatataaa aaggaaagaa aatacgattt
     8281 actattacaa tttagaaggt gagaaaaaaa tattaccagt agaacaagtt gatgatattc
     8341 atatatatgg agaagtagat ttaaacacaa aacttataaa ttacataagt aaatatgggg
     8401 tgatgcttca tttctacaat tactatggat attattcagg tacatattat ccaaggaaga
     8461 aaaatgtatc aggattcagt ttagtatgtc aatcagcctg ctatttagat aaaaatgaaa
     8521 gattatactt agcaaaatcc tttgtggaaa gtgcatcata ccatatttta agaaatatga
     8581 gatattacaa agttgatgaa agtttaattg atactataaa ggatgaacta aatgatttga
     8641 atgatgtcaa agaaatatca gaacttatgg gtaaagaagg cagaataaga aaaacatact
     8701 ataaatcatt taatcaaata ttaaaagatg attttgaatt ttataaaaga gaaaaaagac
     8761 ctcccaaaga cccagtaaat actcttatgt cttttggaaa tagcataatg tatacgaatg
     8821 ttttaagtga gatatataag acacaacttg accctacaat aagcttttta catgaaccat
     8881 caacaaagag attttcttta agtttagata ttgcagaaat ttttaagcca attatagttg
     8941 acacaataat ttttagcctg attaataata aaagaattaa taagaatgat tttgaatatc
     9001 aagacaaaat ttgttattta agtgaaaaag gaaagaagaa atttttgcaa gaatttgaaa
     9061 ataagatgca gacaactata aaacatagaa atctaaatag aaaagtttca tatagaatgt
     9121 ttataaggtt agagtgttat aaattaatta aacattttat aaatgatgaa aggtacaaag
     9181 ttttaaaggt gtggtggtaa gtatgtttgt tattgttact tatgatattg ttgaagcaag
     9241 gtcgttaaat agaattagaa ggatacttag aaaatatttg acttggacgc aaaattctgt
     9301 ttttgaaggc aatattactg atggaaagtt acataaatgt atttctgaaa tagaaaatat
     9361 tattgataat agcgaggatt caatctatgt ttatgagata aaaaatccta attcaattaa
     9421 aaagaaatgt tatgggattg ataagtattc tgatgaaatg tttatatagg tttgcagtga
     9481 gcgatattta tgctaaaata ggtgttaaca gttggaatat aagggattga aggtgtatga
     9541 taactgttat caattgcact actgctcgct cactgcaaat tttgatgttt ttattgaatt
     9601 ataattgctt gattgaagta ttttcaatgt attcaattat acctattttg gggttttata
     9661 ttaactaagt ggtatgtaaa taagcaacta tagtagaagc aaaagcgaca acaactaaag
     9721 gttttatatt aactaagtgg tatgtaaatt tggttaactt aagtgctatg aaaggaatta
     9781 atgaagtttt atattaacta agtggtatgt aaatttaaag gtgcaagtct taatacttgt
     9841 tacatgagtt gttttatatt aactaagtgg tatgtaaata acaaagaacc atttgaatca
     9901 cgaaaactaa aaaacagttt tatattaact aagtggtatg taaatttatt aaccttatca
     9961 gaaacctgac catcccaatt atagttttat attaactaag tggtatgtaa attccatttg
    10021 tctaatgctt ctaactcttt ttttatacgt tttatattaa ctaagtggta tgtaaattcc
    10081 aaaatgggct tggagttaag agttgtataa gaaacgtttt atattaacta agtggtatgt
    10141 aaattaatta cacactccct tttaacgaac atattttaga ttgttttata ttaactaagt
    10201 ggtatgtaaa tagactcgac atagaatcgg ttactaaatc cgagggtttc aggttttata
    10261 ttaactaagt ggtatgtaaa tactaaacct ttttgtttac ttttatttct cattttacgt
    10321 tttatattaa ctaagtggta tgtaaatccg gatttttctg cttcgactgt tgttattgag
    10381 acttgtttta tattaactaa gtggtatgta aatagtaatt ccatgcaatc ttttatattg
    10441 ctttttaacc tgttttatat taactaagtg gtatgtaaat agatatacaa ggtgaaagtg
    10501 ttgaaatttc aatcttgttt tatattaact aagtggtatg taaatattct tagatgttat
    10561 aatttgtgtt gcatttaaat cgttttatat taactaagtg gtatgtaaat acaatattat
    10621 tgttcatttc atttaataat tctagtagtt ttatattaac taagtggtat gtaaatttgt
    10681 ttataccttt aatctttaaa attaaatata aaagttttat attaactaag tggtatgtaa
    10741 atcattgtat actaagcttg atatagtgca aaatgaaacg ttttatatta actaagtggt
    10801 atgtaaatac taattgggtt aaacaaatgg atataactaa attagtttta tattaactaa
    10861 gtggtatgta aatccaaccc ttcgcacgaa tccgtgcgac gaaatataaa gttttatatt
    10921 aactaagtgg tatgtaaatt gtaaaaatat tgcttagcat attgatatca ctaactgttt
    10981 tatattaact aagtggtatg taaatacaag aggtttttca gcaatatatg tacttctata
    11041 atgttttata ttaactaagt ggtatgtaaa ttcatcaata ttaattttgg gaatatcaac
    11101 attttttgcg ttttatatta actaagtggt atgtaaatgc gataagagat tcgagtcttt
    11161 ttactgggct taatcgtttt atattaacta agtggtatgt aaataggata aagaaaagac
    11221 tcacacaaga cacagtgtca ggttttatat caactaagtg gtagataagt agaatattag
    11281 caatatcaac gagtatttag aaacttattt tatattaata aagcagtata taaaattgta
    11341 gaataaacaa tagcatatac taaaacatcc accttatatt aactaaatca tatctaaata
    11401 aaatgcactt acctataaac attataaaat caatacaaaa atgaggtgaa acaaaattta
    11461 tgataaagaa attaaacaat aaagacataa ataaaatcat ggaaatatgg gaaaaaagta
    11521 caatcaaagc acatgacttt ataagtaaag aatactggca aaataactac aatactgtta
    11581 aaaacgaata tatacctata tcagatacat ttgtatatga tgatggagat gaaataaaag
    11641 gatttataag cataatagat aaaagcttta taggagcttt atttatagaa cccaaatacc
    11701 aaaatctagg tatcggaggt aaacttttag attatgcaac taaaaaatat aaaagtctaa
    11761 gtttagcagt atataaagat aataaaaaag cagttgtgtt ttataataaa aaaggtttta
    11821 atatagtaaa agaacaagta aatgaagatt caggatttaa agagtacata
//
LOCUS       DAMJWP010000102        11869 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.106, whole genome shotgun sequence.
ACCESSION   DAMJWP010000102 DAMJWP010000000
VERSION     DAMJWP010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11869
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.106"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(<1..153)
                     /locus_tag="PC700_002246"
     CDS             complement(<1..153)
                     /locus_tag="PC700_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2581551.1"
                     /translation="MKNRLEEIRKERGIKQEELASILQVSRQTIGSLENGRYNPSIIL
                     AFKIARY"
     gene            complement(327..1193)
                     /locus_tag="PC700_002247"
     CDS             complement(327..1193)
                     /locus_tag="PC700_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2581552.1"
                     /translation="MEWINKLNQAITYIEENLESGVDYAEAAKIACCSTFHFQRMFSY
                     IAEVPLSEYIRRRRMTKAAFELQNSNIKILELSEKYGYDSPTSFNRAFQNIHNISPSA
                     ARAKGVVLKAYPKMTLSIYVKGNVEMQYRIVEKKGFRIVGIKESMNMIVEECFEKVPK
                     LWAKCIKNGTIDKLSELINNEPCGLLGVSVCTNSKGLDYYIAAPTDKPVLEDTREYLI
                     PSGTWAVFECVSPMPNALAIQELQKRIITEWLPSSGYVYANLPDIELYPNGDINAPDY
                     TTEVWIPIQKPS"
     gene            complement(1419..2288)
                     /locus_tag="PC700_002248"
     CDS             complement(1419..2288)
                     /locus_tag="PC700_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2581553.1"
                     /translation="MDYKTIILNRKSVREYKNIDIKEEYLSEIKKYSESCKKLVPEID
                     VDVRIMNKSEVFNNLDKVAGYKGNMIDSPSYIIILSDIKDNYIENSGYIGESIIFKVT
                     ELGIGSCWVTFEDGKKVKEKLGINSDKEVTGIIAIGYGENVSNAKVLNATKTGQNYSK
                     SDMQVVTDKGSTRLGVEDVVYMNEWGNVASIEILEERALLDAFHYARLAPSTLNRQPW
                     RFIVDGGTVVLAVRKDGHTNLYEEKIDIGIVMLYFATIISATMFDLKWNLGTPDKDYK
                     VPEDYKIVGYCNI"
     gene            complement(2794..3015)
                     /locus_tag="PC700_002249"
     CDS             complement(2794..3015)
                     /locus_tag="PC700_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581554.1"
                     /translation="MNVNNHKCPECGEHSKKFEIVEENEDCVIFECYTCGTISILKYC
                     ENCKEFVFVNIPNNVETYEYCCGKCNNKI"
     gene            complement(3516..4496)
                     /locus_tag="PC700_002250"
     CDS             complement(3516..4496)
                     /locus_tag="PC700_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581555.1"
                     /translation="MGDTSRFYESVKNKYPKLKDGTRVHLWPKENIIIGPIYDEEDGY
                     KKSIGNVDSLSWKQIVILAECDGKNTTDDIINILSNRYRNIHNMKEKVMEFFMFYENV
                     YLTFEDDIQTLSSVFEITGNKSYITPLYFTIEIDGDNNTNDYFSEITSLLNCMYEKGC
                     RFIEVIGEDILKNKSMREIFQYMLDHFDLIVVTKDSFTIDRSLIKELDNYRHKVIWKV
                     YSKNDDTRIKLKEDIKITSLIKRGHTVVRGDREKAIKIEDMRFKDTKERFSKYGAEWS
                     HLYISSDGSVKSYSFQKDKRVYLGNIFNNSVEEIFLEMQNKIDKLEIVYQ"
     gene            complement(4660..5532)
                     /locus_tag="PC700_002251"
     CDS             complement(4660..5532)
                     /locus_tag="PC700_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2581556.1"
                     /translation="MIIKVSMTVLVLILLYILYKYETQHIETTEYTIFNKKIPKEFDN
                     FKIVQVSDLHNKVFDKNNKVLIDKIESLNPDVIFITGDLVDGENKNFQVALDLIDNLV
                     EKYEVYHIIGNHEQKSLIKKYKHLYKDYFKELYSKKIINMENESVRIYRGNSHLNLYG
                     LIIPLECYPYLFANNKKLELSDNFVEDNLGKVNENEYNILLAHTPFFFEKYAKWGADL
                     VLAGHVHGGIIRIPFVGGVLSPNREFFPKYDWGKYEKDNSTMILNKGLGGSKVLIRLN
                     CKPEIVKITLKYEK"
     gene            complement(5696..6247)
                     /locus_tag="PC700_002252"
     CDS             complement(5696..6247)
                     /locus_tag="PC700_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2581557.1"
                     /translation="MDNKFPNVGENLRLLRQEMGISLDKASKMTGVSKAMLGQIERGE
                     SSPTVSTLWKISSGFKINFTTLLNENTNTYEVIKKEEVEPIVEQKGNMKLYPIYPFSP
                     QRRFELFIIELEENCTHVSSTHSYVLEEYVLVIEGKLDLNVDGKTYILESGHSIRFDG
                     TLEHIYKNLNKGKTIFHNIEVYR"
     gene            complement(6267..7169)
                     /locus_tag="PC700_002253"
     CDS             complement(6267..7169)
                     /locus_tag="PC700_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HDF2581558.1"
                     /translation="MSKKGRVVKVQHFSVNDGDGIRTTIFLEGCKLKCKWCSNPDSWS
                     NIVKLGVMKDKCVSCNRCIDVCPQNISSLFDRTQINNKCDLCGECIKVCLKDAICIMT
                     EKMSVEEIVEEVEKDFIFFFESNGGITFSGGEPTLQIDFLRELVDIFYDKGINIAIET
                     CGYFDWNKVNDVFEKIDHIFVDIKSMDDNIHKEYTGVSNKIILDNICRLSKLNKSMVI
                     RVPIIYGVNDSEENIRNTALFVKQNVPGGKMELLPYHKFGIDKYKALGLEDYIYEFDE
                     ICNNHMLKLKEIVELTGVKIIEYK"
     gene            complement(7184..9556)
                     /locus_tag="PC700_002254"
     CDS             complement(7184..9556)
                     /locus_tag="PC700_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HDF2581559.1"
                     /translation="MSQTNRIEAFRQEYINSKPMICCERARIFTESHKKTEGEAICIR
                     RAKAFLETCKELPIKIFENELIVGTAGKFRRTGILTPEFSWQWVDKEMDTFDKRTQDP
                     YVISKEQIEFIRKEIFPYWKGKSLEEVFLARIPEDTAKILVDTGIIDNDSKWRQAVGE
                     VTPDYQDILFVKGYKGIKEDADKKIKELDISVSENIEKIDFYKSVSIVAEGIMTLAQR
                     YSNLAKEMSKQETDEKRKLELIKISEICMNVPANPPTNFYEAIQFVWFVQLGGILSEN
                     PLALNLGRFDQYMYPYYENDAREGKITESEAQELIEALWIKLSEWVWTISANTANYFA
                     GYNQFQNLTVGGKKRNGTDGTNDISYMCLKATESVKTHQPGLSVRVSQGAPDNFVMAV
                     AKLVKQGTGFPAIHSDSAGAQMLLQDGYDAEDARDWSNCGCVVPHFRKTGQWTSAVNI
                     NFAAALEYAMNEGKSRLTGEKMGLDTKNITEFTSFEELKDEFLKQLAYLVKSSVIGTT
                     VAQQIHKEMVPRPFLSTCVDGCLDKGVDLSKGGAKYNIGPVLTGIGLGVVSNSLAAIK
                     KLVFEDKVTTLEELTKALNNDWEGYEELRKLALDVPKYGNDNDYVDSLAIEVSDFYYT
                     ETRKYKDIFGSKFNSAFMGISNYVPTGKIVGATPCGRKATKPLTEGVSPFVGTDTTSP
                     LAAMKSASKINHDVHTGGTLLNLRLNQDLVETERGLRNLTSMIKSYFALGGFHVQFNT
                     ISNDTLLKAQENPEEYKDLLVRVAGYSTQFVNLSREMQDAIIARNSHSNF"
     gene            complement(9775..10464)
                     /locus_tag="PC700_002255"
     CDS             complement(9775..10464)
                     /locus_tag="PC700_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581560.1"
                     /translation="MKVKRNINRVAIIVALNLSLALMAGCMSPVSDKNLSSSEQISKD
                     SLKEQVEDKKDESIELFNNYSQKINDAKGREEVKKDLSQRFSKAIIDSGYSITDSKSD
                     GSFVVSINGAEKPPEKNIRSLFYSMSEDDKEGKVTIDILCAKEYPEDDKLSESDKYIK
                     FIYNLFKSLTNTNLTEKEIFSMVEEEFNKGVGVVELPYMKDIHVEVNKVKQSTKVLKL
                     SLVYKFDISNH"
     gene            complement(10705..>11869)
                     /locus_tag="PC700_002256"
     CDS             complement(10705..>11869)
                     /locus_tag="PC700_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2581561.1"
                     /translation="AFIAWGGYYNYFYKMDYIKYSIFFYLFSVYYKCLDAVIYRILFF
                     AITGNVISSKESLLVPHLESIKIQIYSNLIIILIYLVFSFFRKSIKLISQDKRNYVYL
                     LFALLANTMNMLVNYVLKKLERFGSLHSEGYYFDNFVNPKLVGASSIFLILLFKEIIK
                     ENRLKSQAELIKNKLDMQYAHYLSIQESHMRVKKLYHDINNHIYCIDNLRNNSKEINE
                     YVNNLKDEIKTFKYIYNTGNMILDIIINEKSEICLKKGIKFTCSINFSKVNFVKPIDV
                     SSIFSNILDNAIEACDKIVDENINKYIRIKGTITKSFFVLKCENSKLNQLTFNKNILL
                     TNKMDKFVHGIGIQSIKSSLQKYNGELLFENSIDKFILNIYIPLEQDTDSWGD"
BASE COUNT         3883 a         2069 c         1143 g         4774 t
ORIGIN      
        1 gtatctagct atcttaaagg ctaagatgat agaaggatta tacctaccat tttctaatga
       61 acctatagtt tgtcttgata cttgcaaaat agaagcaagt tcttcttgtt ttatgcctct
      121 ttcttttctt atttcttcta atctattttt cacatctatc cccctttaat atctcaacta
      181 cggaaagtct actttccata attatacaac tatatttcat ttatgtaaag cttgctttcc
      241 atatttacaa aaaaataaca gactattttc agcctgtaat tttaaaattt catgttgttt
      301 attaaattta atgttattca ttaactttaa cttggtttct gaattggaat ccaaacttct
      361 gtagtataat caggagcatt gatatcgcca ttgggataaa gttcaatatc tggtaagtta
      421 gcatacacat acccagaact tggtaaccac tcagtaataa ttcttttttg caattcctgt
      481 attgccagag catttggcat tggagaaaca cattcaaata ctgcccatgt acctgatggt
      541 attaagtatt cacgagtatc ttctaataca ggcttatctg ttggtgcagc aatataataa
      601 tctaaaccct tactatttgt acacacacta actccaagca atccacaagg ttcgttattt
      661 attaactctg aaagtttatc aattgttcca ttttttatgc atttagccca taacttagga
      721 actttctcaa agcactcttc tacaatcatg ttcatacttt cttttatacc aacaatacga
      781 aaccctttct tttctacaat acgatattgc atttctacat ttccttttac ataaatgctt
      841 aatgtcatct taggatatgc ttttaaaaca acaccctttg ctctagctgc agatggagaa
      901 atgttatgta tattttgaaa agctctatta aaagatgttg gtgaatcgta accatacttc
      961 tcagatagtt ctaaaatttt gatgttactg ttctgaagtt caaatgcagc cttagtcata
     1021 cgtcttcgtc taatatattc agataatggt acttctgcaa tatatgaaaa cattctttga
     1081 aaatgaaagg ttgaacaaca tgcaattttt gctgcttctg cataatccac tccactttcg
     1141 agattttctt caatataagt aatagcttga tttaatttat ttatccattc catttgatat
     1201 tacctccaag caaattatac agtttaataa aataatatac ctctctattc atgcacaaaa
     1261 ttgtaagcat ttaaattcat aagtattttt caatcaatac aataaatata aaaattgtat
     1321 tatacaataa aaaagttcaa taacatttta ttataaaata ttattgaact ttttataaaa
     1381 attaatttaa gtgtagttaa attaagtatt aaactatctt atatattaca atatcctact
     1441 atcttatagt cttctggcac tttataatct ttgtctggag tacctaaatt ccattttaaa
     1501 tcaaacatag ttgcacttat tattgttgca aaataaagca taactatacc aatgtctatt
     1561 ttttcttcat ataaattagt atgaccatct tttctaacag ctaaaactac agtgcctcca
     1621 tcaactataa atctccatgg ttgtctattt aatgtagatg gagctaatct tgcatagtga
     1681 aatgcatcaa gaagtgctct ttcttctaag atttctatac ttgcaacatt tccccattcg
     1741 ttcatataaa caacatcttc tacacctagt ctagtagacc ccttatctgt tacaacttgc
     1801 atatcagatt ttgaataatt ttgcccagtt tttgtagcat ttaatacttt agcattactt
     1861 acattttctc catatccaat agctataatt cctgttacct ctttatctga attaattcca
     1921 agtttttctt ttaccttttt tccatcttca aaagtaaccc aacaagaacc aattccaagt
     1981 tctgttactt taaagattat actttctcct atgtaccctg aattttcaat gtaattatct
     2041 tttatgtcag ataatattat aatataactt ggagaatcta tcatatttcc tttgtaccct
     2101 gctactttat ctaaattatt aaaaacttca cttttattca taattcttac atcaacatct
     2161 atttctggaa ctagtttctt acaagattct gagtatttct taatttcact aagatattct
     2221 tctttaatat ctatgttctt atattctcta acagactttc tatttaatat gattgtttta
     2281 taatccataa ttttatcctc cccaaactag tattgtctta attatatacc cattaatata
     2341 ttattttact catttattta ataaataaag attacaattt tgtaattatt atttattaat
     2401 aatattccac ttttacatta ataatgacca gttgtgcaaa atccattgcc aaccaccaaa
     2461 taaaaacata ttataaaagt aatataaaat aacaaacaaa tcttaacaaa aaattaaact
     2521 ttttttacac gaaagaaggc ttcccgttgc cttctttatt tttataaaaa aataagctgt
     2581 ttctaaaatg atttcacatt tttgaaacag ccaagttatt ctataatgac aactataatt
     2641 tgaggatgtg agggagagtt gtcattataa atttagggta attatattat gttttaagct
     2701 agttcttcct atatttatat aatacatttt atatatgaat tttatatgag attggtataa
     2761 atgatatatg aaattacata tgtttatatt atattaaatt ttattattac attttccaca
     2821 acaatactca tatgtctcca cattatttgg aatatttaca aatacaaatt ctttacagtt
     2881 ctcacaatac tttaatatag atatagttcc acaagtgtaa cattcaaata ttacacaatc
     2941 ctcattttcc tctactattt caaacttttt actatgttca ccacactctg ggcacttgtg
     3001 attatttaca ttcataaata ctcctcctta ttagtatagt tacatttcaa aaacttaatt
     3061 aatgtattct atattactta ttatatattc tataataaat catattctaa tataataaaa
     3121 caaattaaat aaaaaaaaac cataaatttt aaatttatgg attaattctt gtaatgataa
     3181 ctctatttga ggatgtgagg gagggttatc attacaatag ggtattatat tatggttttt
     3241 aagctagtct ttctacacct catatgatac atatgctata tgaattttat atgattttta
     3301 cataaatatt ttatgaaatt taaatttttt agtcattttg aaatttgtaa aaagttctct
     3361 aaaaatccac agcatcataa acagccaaaa tttaagtaaa acatcataaa aaaatttgtc
     3421 caataatttt aacaagtcat ttcacctcat tataattatt gacacaaact taaataaaca
     3481 atctataaat cttatataaa tcttaaactt tacccttatt gatacacaat ttctagtttg
     3541 tcaattttat tttgcatctc taagaaaatt tcttcaacag aattgttaaa tatattgcct
     3601 aaatagactc ttttatcttt ttgaaatgaa taagatttta cactaccatc agaagaaata
     3661 tataaatgac tccactctgc tccatatttt gaaaacctct cctttgtatc tttaaacctc
     3721 atatcttcta tttttattgc tttttctctg tctcctctta ctacagtgtg accccttttt
     3781 attaaactgg ttatttttat atcttctttt aatttaatac gtgtatcatc attttttgaa
     3841 tatactttcc atatcacttt atgacgataa ttatctaatt cttttattaa gcttctatca
     3901 atagtaaagc tatctttagt taccacaatt aaatcaaaat ggtctaacat atattgaaat
     3961 atttctctca tacttttatt ttttaaaata tcctcaccaa taacctctat aaatctacag
     4021 cctttctcat acatgcagtt caataaactt gtgatttcag aaaaataatc atttgtatta
     4081 ttatctccat ctatttctat agtaaagtac aaaggagtaa tgtatgattt atttcctgtt
     4141 atctcaaata cacttgatag tgtctgtata tcatcttcaa acgttaagta tacattctca
     4201 taaaacataa aaaattccat aactttttct ttcatattat ggatatttct atatctattg
     4261 gataatatat ttattatatc gtcagtagta tttttcccat cacattcagc cagtataaca
     4321 atttgcttcc aacttaagct atccacattt ccgatacttt ttttatatcc atcttcttca
     4381 tcataaatag gaccaattat aatgttttct tttggccaca gatgaaccct agttccatct
     4441 tttaatttcg gatatttatt ttttacagac tcataaaatc tacttgtatc acccatgatt
     4501 ctttactccc ccccatagtt taaatattct atgctaaaaa tagcacttta tttttattaa
     4561 ttcaacaatt attcaaaatt ggtcattata tatgtttaaa tggatttaac atcaacctta
     4621 tttatatgtt attgatgttt atatatgtta tataaaatct tatttctcat acttcaatgt
     4681 aatcttaact atctctggtt tacaattaag cctaattaaa acttttgagc ctcctaagcc
     4741 tttatttaaa atcatagtgc tattatcttt ttcatatttt ccccagtcat acttaggaaa
     4801 gaactctcta tttggagata atacacctcc aacaaaaggt attcttatta tacctccatg
     4861 aacatgtcct gctaaaacca aatctgctcc ccattttgca tatttttcaa agaaaaatgg
     4921 tgtatgtgct aaaagaatgt tatattcatt ttcattaact ttacctagat tgtcttctac
     4981 aaaattgtca cttagttcta attttttgtt attagcaaat aaatatggat agcattccaa
     5041 tggtataatt aaaccatata aattaagatg gctattacct ctatatattc ttacagattc
     5101 attttccata tttattattt tctttgaata tagttcctta aaataatcct tatatagatg
     5161 tttatacttt tttattaaag atttttgttc atgatttcca attatatgat atacttcata
     5221 tttttcaacc aaattatcta ttaaatctaa tgcaacttga aaatttttat tttctccatc
     5281 tactaaatct cctgttataa atataacatc tgggtttaat gattcaattt tatctatcaa
     5341 taccttatta tttttatcaa acaccttatt atgtaaatca ctcacttgaa ctattttaaa
     5401 gttatcaaat tcctttggaa tttttttgtt gaaaatagta tattctgtcg tttctatatg
     5461 ctgagtttca tacttataca gaatatacaa taatattaat actaatactg tcattgaaac
     5521 ctttataatc atattctctt cctctttatt ctattttgtt aatattatta taatgctttt
     5581 atgttttttt taaaacaata acatcatcac atacatataa atcttttcga cattataaaa
     5641 ataagttatc cttttctaaa attttcattt attttagaaa aggataacta taaaattatc
     5701 tataaacctc tatattgtga aatatagttt ttcctttatt taaattttta tatatatgct
     5761 ctaaagttcc atcaaaccta atagaatgtc cagactctaa aatataagtt ttcccatcca
     5821 cattaaggtc aagtttacct tctattacga gtacatactc ttctaaaaca taactatgtg
     5881 tagatgaaac atgagtacag ttttcctcaa gctcaattat aaaaagttca aaccttcttt
     5941 gagggctaaa agggtatatt ggatataact tcatgttccc tttttgctca acaataggct
     6001 ctacttcttc tttctttata acctcatagg tatttgtatt ttcatttaat agtgtagtaa
     6061 aatttatttt aaatccagat gaaattttcc ataaagtaga tactgtcgga cttgattcac
     6121 ctctctcaat ctgtccaagc attgctttac tgacacctgt catctttgaa gctttgtcta
     6181 aacttatgcc catttcttgt ctcaataacc ttaaattttc tcccacatta ggaaatttat
     6241 tatccatgtt aaaatcctcc tgatagctat ttgtattcta tgatttttac tcctgtaagt
     6301 tctactatct cttttaattt taacatatga ttgttacaaa tttcatcaaa ttcatatata
     6361 taatcttcaa gccctaatgc cttatattta tcgattccaa atttatgata tggaagtaac
     6421 tccatcttgc ctcctggaac attttgtttg acaaataatg cagtatttct tatattttct
     6481 tcactatcat ttactccata aataattggc actcttataa ccatagattt attcaacttt
     6541 gaaagcctac atatattatc taatataatt ttattactta ctcctgtata ttctttatga
     6601 atattatcat ccatagattt tatatctaca aatatgtggt ctattttctc aaatacatca
     6661 tttaccttat tccaatcaaa atatccacaa gtttcaattg caatatttat gcctttatca
     6721 taaaaaatat caactaattc tcttaaaaaa tcaatctgaa gtgtaggttc acctccagaa
     6781 aaagttatcc ctccattaga ttcaaagaaa aatataaaat ccttttcaac ttcctctact
     6841 atttcttcaa cactcatttt ttcggtcatt atacaaatgg catctttaag acataccttg
     6901 atacactctc cacataaatc acatttatta tttatttgcg ttctatcaaa tagtgaactt
     6961 atattttgag gacaaacatc tatacatcta ttacaggata cacatttatc tttcataaca
     7021 cctaatttta ctatgttact ccaggaatct ggattagaac accacttaca ttttaattta
     7081 catccttcta aaaatatagt agtccttatt ccatctccat catttactga aaaatgttgt
     7141 acttttacaa ctctaccttt tttactcata atctacctca taattaaaaa ttgctatgtg
     7201 aatttctagc tattatagca tcttgcattt ctcttgataa attaacaaat tgagtactat
     7261 atccagcaac tctcactaat aaatctttat attcttctgg attttcttga gcttttaata
     7321 gagtatcatt tgatatcgta ttaaattgta catggaatcc tcctaaagca aaatatgatt
     7381 taatcataga tgttagattt ctaagtcctc tttcagtttc caccaaatct tgattcagtc
     7441 ttaaatttag aagtgttcca ccagtatgaa catcatgatt tatttttgat gcagatttca
     7501 tggctgctag tggacttgtt gtatcagtac caacaaacgg agaaactccc tctgtcaaag
     7561 gttttgtcgc ttttcttcca caaggagttg cacctacgat ttttccagtt ggtacatagt
     7621 ttgatatacc cataaatgca ctattaaatt tagagccaaa aatatccttg tattttctag
     7681 tttcagtata ataaaaatca gaaacttcaa ttgctaatga atctacataa tcattatcat
     7741 ttccatactt aggaacatcc aacgcaagtt ttcttaattc ttcataacct tcccaatcat
     7801 tatttagtgc ttttgttaat tcttctagcg tagtcacctt atcctcaaat actaattttt
     7861 ttatagctgc caatgaattt gaaactacac ctaaccctat accagttaat acaggaccta
     7921 tattatattt tgctcctcct ttacttaaat caactccctt atccaaacat ccatctacac
     7981 aagtagataa aaatggtctt ggtaccattt ctttatgaat ttgttgagca acagtagttc
     8041 ctataacaga actttttaca agatatgcta attgctttaa aaactcatcc ttaagttctt
     8101 caaaagaagt aaattctgtg atattttttg tatctaaccc cattttttct ccagttaatc
     8161 tactcttacc ttcattcata gcatattcta aagctgctgc aaaattgata tttacggctg
     8221 aagtccactg tcctgttttt ctaaaatgag gaactacaca accacagtta ctccaatctc
     8281 tagcatcttc tgcatcatat ccatcttgta ataacatttg tgcccctgca ctatcgctgt
     8341 gtatagcagg gaaaccagtt ccttgtttta ccaatttagc aactgccatt acgaaattat
     8401 ctggtgctcc ttgactcact ctaacactta atcctggctg gtgagttttt acactttctg
     8461 tagcctttaa acacatatat gagatatcat tcgttccatc agtaccattt ctctttttac
     8521 caccaacagt aagattttgg aattgattat atcctgcaaa gtaatttgct gtatttgctg
     8581 atatagtcca tacccattct gataacttaa tccaaagtgc ttctatcaat tcttgagctt
     8641 ctgattcagt tattttacct tctctagcat cattttcata atatggatac atatattggt
     8701 caaatctacc taaatttaat gccaatggat tttctgatag aataccacct aattgaacaa
     8761 accatacaaa ttgtattgct tcataaaaat tagttggagg gtttgctggt acattcatac
     8821 atatctctga aatttttata agttctaact ttctcttttc atctgtttct tgcttactca
     8881 tctcttttgc aagatttgaa tatctttgtg ccaatgtcat aataccctct gcaactatag
     8941 aaacagattt gtaaaaatct atcttttcaa tattttctga aactgatata tctaattctt
     9001 taattttttt atctgcatct tccttaatcc ctttatatcc ttttacaaac aatatatctt
     9061 gataatctgg tgttacttca ccaactgcct gtctccactt agaatcatta tctattattc
     9121 ctgtatctac taatatctta gctgtatctt ctgggattct tgctaagaaa acttcttcca
     9181 aagattttcc cttccaatat ggaaatattt cttttcttat aaattctatt tgttcttttg
     9241 atattacata tgggtcttga gttcttttat caaaagtatc catttctttg tctacccatt
     9301 gccaagaaaa ctctggtgtc aatatccctg ttcttctaaa ttttcctgct gtacctacta
     9361 ttaattcatt ttcaaagatt tttataggaa gttctttaca tgtctccaaa aaagctttag
     9421 ctcttcttat acagatagct tctccctcag tttttttatg tgattctgta aaaattctag
     9481 ccctttcaca acatatcata ggtttagaat taatatattc ttgtctaaat gcttctattc
     9541 tattagtttg actcatttaa aatccccctt tggataatat atactacatt tttgtatgtt
     9601 ataacatact ttaattatat tatactgtcc tttagcataa aatggaagca aaataacttt
     9661 ttttttcatc taccttgaac acctatatat taatactttt tttgttaaat ttataaaatc
     9721 taaaaaaatt ttctatgata tattttctat attgttatag ttatttctaa tatactaatg
     9781 atttgaaata tcaaatttgt atactagact tagttttaaa accttagttg attgtttaac
     9841 tttgttaact tcaacatgta tatccttcat ataaggaagc tccacaacac ctactccctt
     9901 attaaattct tcttcaacca tagaaaaaat ttctttctca gttaagttcg tattagtcaa
     9961 tgatttaaat aaattgtata taaatttaat atatttatca ctctcagata acttatcatc
    10021 ttctggatac tctttagcac agagaatatc tattgttact tttccttctt tatcgtcttc
    10081 agacatagaa tagaataaac ttcgaatatt tttttcaggt ggtttttcag caccatttat
    10141 agatactaca aagctaccat ctgattttga gtcagtaata ctataaccac tgtctattat
    10201 agctttcgaa aatctttgag ataaatcttt ttttacttct tctctacctt tcgcatcatt
    10261 aatcttttgt gagtaattat taaatagctc gatagattca tcttttttat cttcaacttg
    10321 ttctttcaat gaatctttac taatttgttc tgagctactt aagttcttat cagatacagg
    10381 agacatacat cctgccatca atgctaagct aagatttaaa gccacaatta ttgccactct
    10441 atttatattc ctttttactt tcatacacaa tcctcccatc acactaaccg ctctaaagta
    10501 ttattcattg cattagaata tatttatatt ttctttatat taaagattta ttattcaata
    10561 catatcacct tcctatccaa ttcttataac ttactattag taaaattata agctccttaa
    10621 tacagaaata aggatgttat cttttgttat taaaagataa catccttatt tttttgtatc
    10681 aatacatatg tcccaactgg taacttaatc gccccaactg tcagtatctt gctccaatgg
    10741 tatatatata tttagtataa atttatcgat actattttca aataataatt ctccattata
    10801 tttttgtaaa gaagatttaa tactctgaat acctattcca tgtacgaact tatccatttt
    10861 gtttgttaac aatatatttt tattaaaagt aagctgattt agtttactat tttcacattt
    10921 aagtacaaaa aatgactttg ttatagttcc tttaattcta atatatttat taatattttc
    10981 atccactatt ttatcacatg cttctattgc attatccaaa atattagaaa atatgcttga
    11041 cacatctata ggttttacaa aatttacttt tgaaaaattt atagagcaag taaatttaat
    11101 tccttttttt aaacatattt cactcttttc gttgattata atgtctaata tcatatttcc
    11161 tgtattatag atatatttaa atgtttttat ttcatctttt aaattgttaa catattcatt
    11221 aatttctttg ctattgtttc taaggttgtc tatacaataa atgtggttat ttatatcgtg
    11281 atatagtttc ttaactcgca tatgagattc ttgaatactt aaataatgcg catattgcat
    11341 atctaactta ttttttatta attcagcttg agattttaat ctattctctt ttattatttc
    11401 tttaaacaat aatataagaa atatactact agctcctacc aactttggat taacaaaatt
    11461 atcaaaatag tatccttctg aatgtaatga cccaaaccgt tctaattttt ttaatacata
    11521 atttacaagc atattcattg tattagctaa taaggcaaat aataaatata cataatttct
    11581 cttatcttgg ctaattaatt ttatagactt cctaaaaaag ctaaatacaa gatatataag
    11641 aataattatt aaattactat atatctgaat ttttatagat tctaagtgag gtactaataa
    11701 ggattcttta gatgaaataa cattacctgt aattgcaaaa aatagtattc tatatataac
    11761 agcatccaaa catttataat atacactaaa tagataaaag aaaatggaat acttaatata
    11821 atccatctta taaaaataat tatagtatcc tccccaagca ataaatgct
//
LOCUS       DAMJWP010000103        11859 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.107, whole genome shotgun sequence.
ACCESSION   DAMJWP010000103 DAMJWP010000000
VERSION     DAMJWP010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11859
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.107"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            369..1283
                     /gene="queG"
                     /locus_tag="PC700_002257"
     CDS             369..1283
                     /gene="queG"
                     /locus_tag="PC700_002257"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDF2581562.1"
                     /translation="MDNKELKAFCNLIGLNCVGVAGVDKYDNLEKILKDRQQKGYLTG
                     MEEPIIENRINPRHIMKDANSIIVCAFPYHINRQEKNKNSNLSKYCHGKDYHIVVKDF
                     LQQICDYISKTVTDFKYKLFVDNGPLVDRYLAYLSGIGYFGINNNIITDEYGSYVFIG
                     YIVNNYKFEEDKPLEKSCFKCGKCVKYCPGNAILGNYDMNPKKCLSYITQKKGDLSEK
                     EKVVLKNGKKVFGCDICQEVCPHNVEMPTTHILEFKENIIDYLDYDEIQNISNKEFKR
                     RYGDRAFSWRGRNIIKRNMEIILDKYDD"
     gene            1389..3626
                     /locus_tag="PC700_002258"
     CDS             1389..3626
                     /locus_tag="PC700_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2581563.1"
                     /translation="MKYNNTSEINPLDYILGQERAVEAMELGLKINNPAYNIYIAGEP
                     GTGKSTYALKVLNEYASKKNTHKDWCYIYNFENPREPIIVELEKGFGRELKKDMEKLI
                     ESLLEDFKNAFESENFEIEKNRLLDEYEIEKDMLLKQIKKYGEEKGFKLKQSKMGIVF
                     IPLKEEEDESDKSDEEFYKAKRELENMAIQVVYKIRNLEEIAEKAVLELEEEIAKLVV
                     EPHIKRLCEKYENYDKIQTYLENIKKDIIEYMYLFYLDEEELKDKYDKEHFIKYKINL
                     FVDNESSKNKESAPVVVEINPSPANLFGKAEYDYANGNIKTDFTKLLSGAFHRANGGY
                     LVLYADQLLKYTMSWEILKKTLQTKKVILETQTAIKPENMPLDVKLVLIGSHYIYDIL
                     YRYDEDFEKYFKVFVDFDSEMDKNEDNEEGIARFIAYQCDKNNLRHFTKSAVEEVIKF
                     STRLSGDLEKLSTKFNKIMEVVIEGSAYAEFRNSEYTEQCDVKKAISEKRKRINRVET
                     HMDESIENGFTLIETEGRKVGVINGLSVLSTGEYSFGRASRITATTSPGSKGIVNIER
                     EVNMSGSIHNKGVLILGGYLSENFAQDLLLSLNAYVCFEQNYGGIDGDSASSAELYVL
                     LSSLSGVSIKQNIAVTGSINQKGDIQVVGGISEKIEGFYSICKTKGLNGEQGVIIPRK
                     NQRNLVLSDEVNDAVRDGKFKIYTVERVEEAIEILTDVKFEEIKKLVKEKLISFSKIQ
                     TVSKE"
     gene            3823..4875
                     /gene="nth"
                     /locus_tag="PC700_002259"
     CDS             3823..4875
                     /gene="nth"
                     /locus_tag="PC700_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDF2581564.1"
                     /translation="MKNVNLKEINEKDVIVEEDINLKGMDKKDVIEKEEVNKKSIDKK
                     DIIEKEEIDKKNVKKVEVQKEIDKMISKKDEIIDEKDIENFRYIEEDKKSIKVSKKST
                     KKGETEKKTKKSSSNKATQKQSKEVKSKSKIKSESANKKDVNKILDELEKLYPDAKCE
                     LNYGTAFELLIATILSAQCTDVRVNKVTSELFKKYNTARDFANLSIEEISKEIKSCGL
                     YKSKSQKIKDTSEQLCELYDGEVPDSLEKLIKLPGVGRKTAGVVLSNAFNHPAIAVDT
                     HVFRVSNRIGIVDEPNPQKTEFALMEAIPKERWSHSHHVLIFHGRRMCKARNPECASC
                     PIKEDCNYYKELNETK"
     gene            5069..5539
                     /gene="trmL"
                     /locus_tag="PC700_002260"
     CDS             5069..5539
                     /gene="trmL"
                     /locus_tag="PC700_002260"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDF2581565.1"
                     /translation="MSLNIVLVEPEIPQNTGNIIRTCAATGSTLHLVKPLGFSLDDKH
                     LKRCGLDYWDIADIQYYDSFEELQEKYPNSKYFFSTTKAKQSHSDVKYEENCFIVFGK
                     ETKGLPEPLLKANMDTCIRVPMLDVEKARSLNLSNSVAIVVYEALRQIGYPNMR"
     gene            5575..6423
                     /locus_tag="PC700_002261"
     CDS             5575..6423
                     /locus_tag="PC700_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDF2581566.1"
                     /translation="MKNLKIICDSLSDVPKDLLDKYDIDIVSLTVILEEREYKDRIDI
                     SGDEFYKRLREENVYPKTSQATYAQFKEIFEKYTNEDREILYIAGSATATGTYQSAVM
                     AKNDVDGKIYTYDSNLLCMGTGLLVIKAGELASEGKSVDEILPVLDELKEKAFVIFSV
                     DTLEYLQKGGRISSTKAAIGSILNIKPILEIRDGLVSQISQVRGKKNVISKMMELIKS
                     NCGDDLSDQIVYIGYSDDFKEKEQLSKVMQDELRPKEIRFFQIGVCIGAHAGPGVTGI
                     ICLKNK"
     gene            6796..7800
                     /locus_tag="PC700_002262"
     CDS             6796..7800
                     /locus_tag="PC700_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2581567.1"
                     /translation="MRFKRILILMLTVAMIAGMLVGCANNPSNNPSSSKDNKSDISDK
                     SNSKKSVSMILDIEGTNNEAMNNAALLALNNAQKKLNIDTNKVESDDSSTFSNSIDIL
                     CNDNYDLIIAVGARFAKPLEMVAKKYPKQQFAIIDYEYDKQPSNITSISYEDNKSGYL
                     AGLIAGKMTESDKVGFIGGIKSSSRDKFESGFREGVKFSNSSIKDISVEYADVFKDSK
                     SVESIAKKMMDNGVDIIFSTTEDDSKAVIDAVRAKNKKVIATNKDQHELAPENVISSI
                     VKDFENPTYNLIQSFVKGNYKGGKVIENTVKSGSTGLAQNSSQNIPPDVLEYVNKNNK
                     "
     gene            7925..9037
                     /locus_tag="PC700_002263"
     CDS             7925..9037
                     /locus_tag="PC700_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2581568.1"
                     /translation="MKIKRILMACFVVVFTIFILAICNFDFGFDKVNKNNSNIDSAKS
                     KQSESKSVPTSAEKDKLNSKSKNEEDDTEDDSYVRKTIGVLLDTENLNNESYNNNVLS
                     ALKRADEDFDVRIKLEKPKTSKSFKESIEKLHKYEPDLIIAVGARFAEPIKEAATKYP
                     KQQFAIVDYTYKVQPANVISVSFQDNVTGYLAGLIAGKMTDTGKVGFIGGVEGSSRDK
                     YESGFRKGLKTSNKDAELLVKYSNIFENPESARTIAKEMRDSDRDVVFSATEDDSKRA
                     IQVARENGGKVIAINKDQYDMAPDNVISSIIKDVEQPVYELIKNVAKDGFKGSKVMEF
                     KIKDGELGLTSKSSRNIPPDVLEYIKKEYNKVKDTY"
     gene            9460..10221
                     /locus_tag="PC700_002264"
     CDS             9460..10221
                     /locus_tag="PC700_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581569.1"
                     /translation="MEFTREPFILDDDLCLALVDKRISYLMENELKLRNIEIIKTIEC
                     KELYESIKYHPDICICNLGKGDIIVAPNVYNQYKELLSRYKFNIIKGEANLLNRYPYD
                     IPYNIAIVGDYAIHNFKYTDKAILDYIENNKLKKINIEQGYSKCSICIVDRTSIITSD
                     KGIWKSMKNTEIECLLIEKGHINLFEMNYGFIGGCTGLISKDKLAFCGDVKKHPDYER
                     IKSFVESKNKEIVTLSCENLLDIGSIVPLMTRKER"
     gene            10224..11042
                     /locus_tag="PC700_002265"
     CDS             10224..11042
                     /locus_tag="PC700_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HDF2581570.1"
                     /translation="MVEKDNISSMKFLRNNNVNDRLIYTNDIVEVVIDSAKQEVLNKK
                     ILNGTKAEQRALNIDEISTEITSMIISIMKEKLLKEYILKCYGRTYPEDKNNIYIYSL
                     NIFAKKEIFMRETVFTRVRNYIEENDFINVEGFIRFRMREFMKYISAIGDIAVEEYLI
                     KKDQDEFIRVLKYFIDTQEEKIDLLKVHIMEDNTFVLYDKNGNKIDSIDDEEIINMVI
                     RENLNYEDFLISTLLTLCPKKIEILDLLNNNCSKEIIDTVEAIFENKVSIIMEN"
     gene            11222..11824
                     /locus_tag="PC700_002266"
     CDS             11222..11824
                     /locus_tag="PC700_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HDF2581571.1"
                     /translation="MDNLIRILILAVIGGFIGYVTNVVAIRLIFRPIEPIKIPILNIE
                     IVGLIPKRRAEIAANVGEIIQEEFLSMDEILANIITDEDKEEVVRYIKARVKIIIHEK
                     VSFIPSGIKNMIQDYLGEIIESEVKQSIDELSKNIINKANERIDIQKMVEDKINELDL
                     YELEEIIIRIAKKELKHIEFLGLVLGFLIGIAQGLITMFI"
BASE COUNT         5017 a         1056 c         2209 g         3577 t
ORIGIN      
        1 ataaaaaaga ctaagtattc aaaagagtta gtaggtaaaa taattaaata gataaaaaga
       61 ctaagtattt aaaggagtta agcagataaa aaagttaaat aagtaaatga aaaagacgaa
      121 gtatctaaaa gagttagtaa ataaaaaagt taaataagta aataaaaaag atgaagtatc
      181 taaaagagtt agtaggtaaa aaactaaata ggtaaaaaga cgaagtatct aaaagagtta
      241 gtaggtaaaa aagttaaata aataaaaaag acgaagtatt taaaagagtt agtagctaga
      301 aaaggttaaa aagactaaaa aaattaaata aatttaaaaa gtcaattaga ttaaaaagga
      361 ataattttat ggataataag gaattaaaag cattttgtaa tttaatagga ttaaactgtg
      421 taggagtagc aggggtagat aagtatgata atttagaaaa gatacttaag gacagacaac
      481 aaaaaggtta tttaactggt atggaagaac caataattga gaacagaatt aacccaagac
      541 atataatgaa ggatgctaac tctataatag tctgtgcatt tccatatcat ataaatagac
      601 aagaaaagaa taaaaattca aatttatcaa aatattgtca tggaaaggat tatcacattg
      661 tagtaaagga ctttttacaa caaatttgtg attatatttc taaaacagta acagatttta
      721 aatacaagtt atttgttgat aatggaccat tagttgacag gtatttagct tatttatcag
      781 gaataggata ttttggaata aataataata tcataactga tgaatatggt tcttacgtat
      841 ttatagggta tatagtaaat aattataaat ttgaagaaga taaaccacta gaaaaaagtt
      901 gttttaaatg tggtaagtgt gtaaaatatt gtccgggaaa tgctatattg ggaaattatg
      961 atatgaaccc caaaaaatgt ttatcatata taactcaaaa aaaaggagat ttgtcagaga
     1021 aagaaaaagt tgttttaaag aatggcaaaa aggtatttgg atgcgatatt tgtcaagaag
     1081 tatgtccaca taatgttgaa atgccaacaa ctcatatttt ggaatttaaa gaaaatataa
     1141 tagattattt ggattatgat gagatacaaa atatttcaaa taaagaattt aaaaggagat
     1201 atggagatag agcttttagc tggagaggga gaaatataat aaagagaaat atggaaataa
     1261 ttttagataa gtatgatgac taataataag agtctgtttc atatacttta atgtgtattt
     1321 actttaacac ttgttgtgta aaagtcatat tatatcatta aagttaaata tggggaggtg
     1381 ggggaatatt gaaatataat aatacatctg aaataaatcc actagattat atattgggtc
     1441 aagaaagagc tgtagaagct atggaattag ggttgaaaat aaataatcca gcgtacaaca
     1501 tatatatagc tggtgaacct ggaactggta aaagtactta tgccttaaag gttttaaatg
     1561 aatatgcatc caaaaaaaat acacataaag attggtgtta tatatataat tttgaaaacc
     1621 ctagagagcc tattatagtt gaattagaaa aggggtttgg aagagaactt aagaaagata
     1681 tggagaaatt aattgaaagt cttttagaag attttaaaaa tgcgtttgaa agtgaaaatt
     1741 ttgaaataga aaaaaataga cttctagatg aatatgaaat agaaaaagat atgcttttga
     1801 agcaaattaa gaaatatggg gaagaaaaag gatttaaatt aaaacagagt aaaatgggaa
     1861 tagtttttat ccctttgaaa gaagaggaag atgaaagtga taagtctgat gaagagtttt
     1921 acaaagctaa aagagaacta gaaaatatgg cgatacaggt cgtatataaa attagaaact
     1981 tagaagaaat agctgaaaaa gcagtgttag agcttgaaga agagatagct aaacttgtag
     2041 tagaacctca tatcaagaga ttgtgtgaaa aatatgaaaa ttatgataag attcaaactt
     2101 atctggaaaa tataaagaaa gatataatag aatatatgta tttgttctat ttagatgaag
     2161 aggagttaaa agataagtat gataaagagc attttattaa atacaaaatt aacctatttg
     2221 tagacaatga aagctcaaag aataaagaat cagcacctgt tgtagtagag ataaacccaa
     2281 gtccagcaaa tctatttgga aaggcagagt atgattatgc aaatggaaat ataaagactg
     2341 atttcacaaa actattatca ggagcatttc atagggctaa tggtggttat ttagtacttt
     2401 atgcagacca attactaaaa tatacaatgt cttgggaaat tcttaaaaag actttgcaga
     2461 ctaaaaaagt aatattggaa acacaaacag caattaaacc agaaaatatg ccattggatg
     2521 ttaaattagt actgattgga agccactata tatatgatat tttatataga tatgatgaag
     2581 attttgaaaa atattttaaa gtatttgtag actttgatag tgaaatggat aaaaatgaag
     2641 ataatgaaga aggaatagca agatttatag cataccaatg tgataagaat aatttaaggc
     2701 attttactaa atcagcagtt gaagaggtta ttaaatttag cactagatta tctggtgatt
     2761 tagaaaaact atcaactaag tttaataaaa taatggaagt tgtaatagaa ggaagtgctt
     2821 atgcagagtt tagaaattcg gagtatacag agcagtgtga tgttaaaaaa gcaataagtg
     2881 aaaagagaaa aagaatcaat agagtggaaa cacatatgga tgaatcaatt gaaaatggat
     2941 ttactctaat agaaacagag ggaagaaaag taggagtgat aaatggtctg tcagtcttaa
     3001 gtacaggaga atactctttt ggtagagctt caaggattac agcgaccact agcccaggaa
     3061 gtaaaggcat agtaaatata gaaagagaag taaatatgag tggctctata cataacaaag
     3121 gagtgcttat attaggtggc tatctttctg agaattttgc acaggattta ctattgtcac
     3181 taaacgcata tgtttgtttt gaacaaaatt atggtggaat agatggagat agtgcatctt
     3241 ctgcagaatt atatgtacta ttatcatctc ttagtggagt atcaattaaa caaaatattg
     3301 ctgttacagg ttctataaat caaaagggtg atatacaagt agttggtggt ataagtgaaa
     3361 agatagaagg attttattct atatgtaaaa ctaaagggtt aaatggtgag caaggcgtca
     3421 taattcctag aaaaaatcaa agaaatctcg ttttatcaga tgaagttaat gatgcagtgc
     3481 gagatggaaa atttaagata tatactgttg aaagagtaga agaggctata gagatattga
     3541 cagatgttaa atttgaagaa ataaagaaat tggtaaaaga aaaattaata tctttcagca
     3601 agatacaaac tgtgtcaaaa gaataagttt attatagaat ttaatgggta tttatgtata
     3661 ataaaaagca agaactttaa taggtttttg ctttttttgt aaatatatat aaattaaatt
     3721 cttattttgt atgttttata tattgatgtt tattttttgt tgatatgact aaactattta
     3781 taaatagtgt tttataaatt ggtattttaa agggggataa ttatgaaaaa tgtaaatcta
     3841 aaagagataa atgaaaaaga tgtaattgtg gaagaagata taaatctaaa aggaatggat
     3901 aagaaagatg taattgagaa ggaagaagta aataaaaaaa gtatagataa gaaagatata
     3961 attgagaaag aagagataga taaaaaaaat gtaaagaagg ttgaggttca aaaagaaata
     4021 gataaaatga ttagtaaaaa agatgagata attgatgaga aagatataga gaattttagg
     4081 tatattgaag aagataaaaa aagtatcaaa gtaagtaaaa aaagtactaa aaaaggtgaa
     4141 acggagaaaa aaacaaagaa aagtagttct aataaggcta cacaaaaaca aagcaaagaa
     4201 gttaaaagta agtctaaaat taaaagtgaa tcagccaata aaaaagatgt aaataagatt
     4261 ttagatgagt tggaaaaatt gtacccagat gctaaatgtg agttaaatta tgggacagcc
     4321 tttgaacttt taatagcaac tattttatca gcacaatgta ctgatgtaag agtgaataaa
     4381 gttacttcag agttgtttaa aaagtacaac acagctagag actttgctaa tttgagcatt
     4441 gaagaaatta gtaaagagat aaagagttgt ggtctttaca aaagcaagtc tcaaaaaata
     4501 aaggatacat ctgaacagct atgtgaatta tatgatggtg aagtacctga tagcttagaa
     4561 aagcttataa aattacctgg tgttggaaga aaaacggcag gggttgtact tagtaacgct
     4621 tttaatcatc cagcaatagc tgtagatact catgtattta gagttagcaa tagaatagga
     4681 atagtggacg agccaaatcc tcaaaaaaca gaatttgcac ttatggaagc tataccaaaa
     4741 gaaagatggt cacactcaca tcatgtatta atttttcatg gaagaagaat gtgtaaagct
     4801 agaaatccag aatgtgcaag ttgcccaata aaagaagatt gtaattatta taaagaactt
     4861 aatgaaacga aataactttt agaaagtaag ttgtacaaga gggattttct gatataataa
     4921 tacttgtgaa aatgttagat aaaaaactat tatatacatg aaataaaaat tattattaac
     4981 attattttat ataatagtat aatattattg ttattctgtt tataaaataa aatttgataa
     5041 ataaaagcga tacttaggga ggattaaaat gtcattaaat atagtattag tagaaccaga
     5101 aatacctcaa aatacaggaa atataataag aacctgtgca gcaacaggtt caactttaca
     5161 tttagtaaaa ccactgggtt tttcattgga tgataaacac ctaaaaagat gtgggcttga
     5221 ctattgggat atagctgata tacaatatta cgatagcttt gaagaattac aagaaaagta
     5281 tccaaatagt aaatactttt tttctacaac aaaggctaaa cagtcacatt cagatgtgaa
     5341 atatgaagaa aattgtttta tagtatttgg gaaagaaaca aagggattac cagaaccttt
     5401 gttgaaagca aatatggata cttgtattag agtgcctatg ttggatgttg aaaaggctag
     5461 gtcattaaat ttatcaaatt cagttgctat tgtagtgtac gaggctttaa gacaaatagg
     5521 atacccaaat atgaggtagt ttcaacaagc ctaaatgaga aaggagcata cttaatgaaa
     5581 aatctaaaga taatctgtga cagtttatcg gacgtgccaa aagatttgtt agataaatat
     5641 gatatagata tagtttcatt aacagttata ctggaggaaa gagaatacaa ggatagaatt
     5701 gatatatctg gagatgaatt ttataaaagg cttagagaag aaaatgtata tccaaaaact
     5761 tctcaagcta cttatgccca atttaaggaa atttttgaga aatatacaaa tgaggataga
     5821 gagatacttt atatagcagg gtctgccaca gctacaggga cgtatcaaag tgctgtaatg
     5881 gcaaagaatg atgtggatgg taaaatatat acatatgact ctaacttgct ttgtatggga
     5941 actggtttac ttgtaataaa agcaggagaa cttgcaagtg aaggaaagtc tgttgatgaa
     6001 atattacctg ttttggatga attaaaagag aaggcttttg ttatattttc agtagataca
     6061 ttggaatatt tacaaaaggg aggtagaata tcatctacta aggcagcaat aggaagtata
     6121 ttaaatataa aaccgattct tgaaataaga gatggactag tttcgcaaat atctcaagta
     6181 agaggaaaga agaatgtaat ttctaagatg atggaattga taaaatcaaa ttgtggggat
     6241 gatttatcag accaaattgt gtatatagga tatagtgatg attttaagga aaaagaacaa
     6301 ttatctaagg ttatgcaaga tgaattaaga cctaaagaaa ttagattttt ccaaattggt
     6361 gtgtgtatag gtgcgcatgc tggtcctggt gtcactggta tcatatgctt aaaaaataaa
     6421 taatatattt ttggatgaca ttatttattt ttttattaaa ttaggataga atattaatta
     6481 ttgagatgtt aaattggatt tattttggat gaaattaagt gttgatatat tagatttagt
     6541 ttgtagggaa gtgtgtaaat agataagaat ctattgcaca cttttttata gtaagattta
     6601 ttaaaaaaat ttcaaaatgt acttgtaaat tgtatataaa aaaattataa ataggatgta
     6661 aaagtaaata ggatgtaaaa gtaaatagga tgtaaaagta aataggatgt aaaagtaaat
     6721 aggatgtaaa agtaaatagg atgtaaaagt aaataggatg taaaaatatc gtaaatgtat
     6781 gaggagtgaa atttaatgcg tttcaaaaga atattgatat tgatgttaac tgtagcaatg
     6841 atagctggaa tgttggttgg gtgtgctaat aacccaagca ataatcctag ctctagcaaa
     6901 gataataaaa gtgatataag cgataaaagt aattcaaaaa aatctgtatc aatgatacta
     6961 gatattgaag gaacaaataa tgaagctatg aataatgcag ctttattagc attaaacaat
     7021 gctcaaaaga aacttaatat agatacaaac aaagtggaat cagatgattc ctcaactttt
     7081 tctaatagta tagatatttt atgtaatgac aattacgatt taattatagc tgtaggagct
     7141 agatttgcaa aaccattgga gatggtagcc aagaaatatc caaagcaaca atttgcaatt
     7201 atagactatg agtatgacaa gcaaccttct aatataactt caatttctta tgaagataat
     7261 aaatctggat acctagctgg attaattgct ggaaaaatga ctgaaagcga taaggttggt
     7321 tttataggag gaataaagag ttctagtagg gataaatttg aatcaggttt tagagagggt
     7381 gttaaatttt ctaatagtag tataaaggat atttcagttg agtatgcaga tgtttttaaa
     7441 gatagcaaat cagtagaatc tatagctaaa aaaatgatgg ataatggagt ggatattatt
     7501 ttttctacta ctgaagatga ttcaaaagct gtaatagatg cagtaagggc aaaaaataaa
     7561 aaagttatag caacgaataa agaccaacat gagttagctc cagaaaatgt tataagttct
     7621 attgtaaaag attttgagaa tcctacttat aatctgatac aaagttttgt aaaaggaaat
     7681 tataaaggcg gaaaagttat agaaaacaca gttaagtctg gaagtactgg gcttgcccaa
     7741 aatagttctc aaaatattcc tccagatgtg ttagaatatg ttaataagaa caataaataa
     7801 tctactataa tatccaagaa tacttatgac tttagttatg aggtgaattg gatgttataa
     7861 tagacatata tagaaagacg tatgcagagg taggcagacc tatctaaatc agggagtgaa
     7921 ttttgtgaaa ataaaaagga tattgatggc ttgctttgta gtagttttta caatttttat
     7981 attagctata tgtaactttg attttggatt tgataaagta aataagaata attctaatat
     8041 agatagtgct aaatcgaaac agagtgagtc taaaagtgta ccaacttcag cagaaaagga
     8101 taaattaaat agtaaaagta agaatgaaga agatgacaca gaagatgatt catatgtaag
     8161 aaaaactatt ggagtattgt tagataccga aaacttaaat aacgaatcat ataataataa
     8221 tgtactatca gccttaaaaa gggctgatga agattttgat gttaggataa aacttgaaaa
     8281 accaaagact tctaaaagtt ttaaagagag tatagaaaaa ctacataagt atgaaccaga
     8341 tttgataata gcagttggag ctagatttgc agagccaatt aaagaagctg ctactaaata
     8401 tcccaaacag caatttgcaa tagttgacta tacttataaa gttcagccag ctaatgtaat
     8461 ttctgtatca tttcaagata atgttactgg atatttagca gggcttattg caggtaaaat
     8521 gactgatact gggaaagtcg gttttatagg cggagttgag ggttctagta gagataagta
     8581 tgaatctgga tttagaaagg ggcttaaaac ttcaaataaa gatgctgagt tactagttaa
     8641 gtattcaaat atatttgaaa accctgaatc agctagaact attgcaaagg aaatgagaga
     8701 ttctgataga gatgttgtat tttcagctac agaagatgat agtaaaagag ctattcaggt
     8761 agcaagagaa aatggtggta aagttatagc tattaataaa gaccaatacg atatggctcc
     8821 agataatgta ataagttcaa ttataaaaga tgtagaacaa cctgtctatg aactgataaa
     8881 gaacgttgca aaagatggtt ttaagggaag taaagtaatg gaatttaaaa taaaagatgg
     8941 agaacttggc ttgacttcaa agagcagtag aaatatacca ccagatgttt tggaatacat
     9001 aaaaaaagag tataacaaag tgaaagatac atattaatta ttgatgtaga agattataaa
     9061 aatatactgt ttaagtttag attagcaata aaaagatata tttattaaat ttgtgctaac
     9121 tgtacaaaat atattgatta agtttagatt ggtgataaaa agatatattt attaaattta
     9181 gtctaactgt acaaaatata ttgattgagt ttagattagc aataaaaaga tatatttatt
     9241 aaatttgtgc taactgtacg gaatatactg cttaagttta gattagtgat aaaaagatat
     9301 atttattaaa tttgaaccaa ctgtacaaag ctatactaat taagcttaga ttaactatat
     9361 aaaattataa caataagtgt gtgggcagaa aaattctgct acacacttta tttatattca
     9421 tataaaaaaa ttatcaaaaa aaagaaaaag gagtaaaaaa tggaatttac aagagaacca
     9481 tttatactag atgacgattt gtgtctagct ttggtggaca aaagaattag ttatctaatg
     9541 gaaaatgaat taaaattaag aaatatagag ataataaaaa caatagaatg taaagaatta
     9601 tatgaaagca taaaatatca cccggatata tgtatttgta atttaggaaa aggagatata
     9661 attgttgcgc cgaatgtata taatcaatac aaagagttat taagtagata taaatttaat
     9721 ataattaagg gagaagctaa tttattaaat agataccctt atgatatacc ttacaatatt
     9781 gcgatagttg gtgactatgc tatacacaat tttaagtata ctgacaaagc aatattagat
     9841 tatattgaaa ataataaact taaaaagata aatatagagc agggctattc taaatgctca
     9901 atctgtatag tagatagaac gtctataata acatcagata aaggtatttg gaaaagtatg
     9961 aagaatacag aaatagagtg tttattgata gaaaaaggtc acataaattt atttgagatg
    10021 aattatggat ttataggtgg atgtacaggg ttgatttcta aggataaact agctttttgt
    10081 ggagatgtta aaaaacatcc tgactatgaa cgtataaaaa gttttgtaga aagtaaaaac
    10141 aaggagatag ttacacttag ctgtgaaaat ttactagata taggttctat agttcctctc
    10201 atgacaagaa aggagcgata actttggttg agaaggataa tatttcttcc atgaaatttt
    10261 tgaggaataa taatgtaaac gatagattaa tatatacgaa tgacattgta gaagtagtga
    10321 tagattctgc aaagcaagaa gtgttaaata aaaaaatact taatggtact aaagctgaac
    10381 aaagagcttt aaatattgat gagatatcaa cggaaataac tagtatgata ataagtataa
    10441 tgaaagagaa acttttgaaa gaatacattc taaaatgcta tgggcgtaca tatcctgaag
    10501 ataaaaataa tatatacata tattcactga acatatttgc taaaaaagag atttttatga
    10561 gagaaacagt atttactagg gtgagaaatt atatagaaga aaatgatttt ataaatgtag
    10621 aaggttttat tagatttaga atgagagaat ttatgaaata tatttctgct ataggtgata
    10681 ttgcagttga ggagtattta ataaagaaag accaagatga atttataaga gttttaaaat
    10741 actttataga tactcaagaa gaaaagattg atttgcttaa agttcatata atggaagata
    10801 atacatttgt tttatacgat aaaaatggaa ataagataga cagtatagat gatgaagaaa
    10861 ttataaatat ggtaataaga gaaaatttaa attatgagga ctttttaata agtaccttat
    10921 taactctatg tcccaaaaaa atagaaatat tggatttgtt aaataataat tgctcaaagg
    10981 aaattataga tactgtagaa gcaatttttg aaaataaagt tagtataatt atggaaaact
    11041 agaatctaag catataaaag ctagccatat aaaattaaaa aaattctttt taaatcgaca
    11101 caaaagactt ataattgtat ataaagctat agtataacta gagttattaa gtataaagaa
    11161 tatctacttg attaatgcaa tatagctggc aaatatacaa ttgataagga gtaattttat
    11221 aatggataat ttaattagga tactgatatt agctgttatt ggtggattta taggttatgt
    11281 tacgaatgta gttgctataa gactgatatt tagaccaata gagcctatta aaatacctat
    11341 tttaaatata gaaatagtag gtcttatacc aaagagaaga gcagaaatag cagcaaatgt
    11401 aggagaaata attcaagaag aatttctttc tatggacgaa atacttgcaa atatcattac
    11461 tgacgaagat aaggaagaag ttgtaagata cataaaagca agggtaaaaa ttataataca
    11521 tgagaaagtt tcatttatcc cgagtggtat aaaaaacatg atacaagatt atctaggaga
    11581 gataattgag tctgaagtta agcaaagtat agatgaattg agtaaaaata taataaataa
    11641 agcaaatgaa cgtatagata tacaaaaaat ggtcgaagat aagattaatg aactagattt
    11701 atatgaattg gaagagataa taataagaat agctaagaaa gaattaaaac atatagagtt
    11761 tttggggctt gtcttagggt ttttaatagg tatagctcaa gggttaatta ctatgtttat
    11821 ttaaatatat gagaagtagc atttatttaa gtactaatt
//
LOCUS       DAMJWP010000104        11781 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.108, whole genome shotgun sequence.
ACCESSION   DAMJWP010000104 DAMJWP010000000
VERSION     DAMJWP010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11781
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.108"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            297..818
                     /locus_tag="PC700_002267"
     CDS             297..818
                     /locus_tag="PC700_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2581572.1"
                     /translation="MEEIVKKEQASMNDIFLYLKAKSNSLYKFVILYNEFINKPKDYG
                     TGELINMVEVHMLTAIEENPGISPTQLAKIWNRTKGAISQTISKLEKKGYITKEKKEG
                     NAKTILLYPTTSGVELSNAHKLYDTIDVTRTFEYLIKECTLEEIDSFYKVIDLFINLL
                     EESPQENNDSEKV"
     gene            1197..2372
                     /locus_tag="PC700_002268"
     CDS             1197..2372
                     /locus_tag="PC700_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2581573.1"
                     /translation="MNLKQLSEKYEEYVIQMRREFHKYPELSFKEIRTTQRIREELQK
                     IGVPFVSIEPNIVIATIQGKNEGKTIAIRGDIDALPMQEDSDVEYKSEYDGIMHSCGH
                     DAHGAMLLGLAHMLNDIRKDLKGRVLLVFQAAEEVGGGHKEIIQYFKENGGPDRLIAT
                     HVWSDIEAGKISVEPGPRMAGGSGWRIDITGRGGHGSRPDQSIDPIKPACDIVLKVSS
                     IPVNHISVLEQCVVHACAIHGGTTIGNVIPNNAEVIGGYRYFTEESGKKVFDIIKQMS
                     NGVGQAYGVDVKVTHTGGIGPVINDEEAVKMAKEIVSNIDGLELDSYEPICASDCYGE
                     ILKEYSGFYCYLGVGNKEKGIIYAQHHPKYNIDEDTLKLGCEFMAKYAVEFLNKNNN"
     gene            2407..3945
                     /locus_tag="PC700_002269"
     CDS             2407..3945
                     /locus_tag="PC700_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HDF2581574.1"
                     /translation="MQGALKENKKKSGGGFLGWIERVGNKMPHPLALFTYITLIVVAL
                     SAIASFLGFSATSPATGKLIKVMNLISAKGLVLFMQEFVKNFQNFPVLGVVLVMAVAT
                     GVCEKTGFFSTAIKMSLSKVKGNAVVFIIAFIGVFANQAGDAAFVLVPTLAGAIFYGL
                     NRNPLAGIFCGFASVGGGFATAVIPGGWDVTLTPITVSAAQTIQPAFDMTLLASYYAL
                     FVSAFIVATVSTIVTVKFIEPKLGKYTGKPEGIDDNQGFEVTKEQAKAAKLAGFTVLA
                     YVALLIALCIPANSFLRSPEGSLIFGAPLMSCLQFFIVILFFLPGIVYGMRVGKIKTV
                     KDLNDILCQSMAAMAPFIVLAIVIGQFLTLFSVSNLAQVLAIKGGALLNSLPLPPQVI
                     ILLFLVLVAFINIFVISGSTKWMIFGPVFVPMLMQLNIHPAFTQFVYRLGDSVTNHLS
                     PLNAFFIILLATVQKYDKNAGMGTIFSAMIPYTIGYFVVLAVLVVVWMTIGIPVGIGG
                     QVWL"
     gene            3977..5179
                     /locus_tag="PC700_002270"
     CDS             3977..5179
                     /locus_tag="PC700_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2581575.1"
                     /translation="METTEIRYNFDEVIDRSGTNCLKYDLKSQFMPESPEDALPLWVA
                     DMDFACAPEIINAMHERIDRKIFGYSSQQTDDYYEAVCGWFKRRFDWKIEKENIFFSP
                     GVVPALGFFIQILTQKGDGIIIEKPVYYPFEDKITNNERKVVNSPLKFEDGHYSMDYD
                     GFEELAKDENNKVFILCSPHNPVGRVWKKEELRKIVDICKKYDLWIISDEIHCDIIRK
                     SEKHIPLEVACPDYKDRIITCTAPSKSFNLAGMQLSNIVINNKELQEKWMLETDSKLA
                     IFLPNPFAIVATKVAYNDCEDWMNQVNEYIDGNIKYVEDFVNEYMPKVKIIKSQGTYL
                     IWLDFRGYGLDAKQLEYVMLNKAKVLLDEGYIFGEEGAGFERINVASPRTIIEECMNR
                     IKCAFEGL"
     gene            complement(5358..5957)
                     /locus_tag="PC700_002271"
     CDS             complement(5358..5957)
                     /locus_tag="PC700_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2581576.1"
                     /translation="MNDNIVEVKNINMNFYTKEGELEVLKDVNFNLKEGEILTLLGPS
                     GSGKSTILNILTNLLKPTSGDIKITGNIGYMFQKDNLLEWRNIMDNITIGLEIQGKKD
                     KKSLDRVESLLKTYGLWDFRSMYPKELSGGMRQRVALIRTLSVNPDILLLDEPFSALD
                     YQTRLLVCDDVYSIIKNENKSTILVTHDIGEARCIKVQL"
     gene            6126..7484
                     /locus_tag="PC700_002272"
     CDS             6126..7484
                     /locus_tag="PC700_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581577.1"
                     /translation="MSNIITNLIESVFTNPITILFLLAITVYSVFKIIENNRVYSFVS
                     SKLDEINRDYKTSEFYKKVRDDYYTYSKENPYADVNITSFIEEVVSDLKHNNLPLLEK
                     IRSIKNSSSISILLGVLGTFVGLSTMLLCVDTKDIINSLPSTISSMQTAFTTSIFGVV
                     FSLIIGYFTKIKDCEHVLIQIMLKSENLLTSEITHFKSERMDLKVEEVKNTIKQISKS
                     IEAIEKFDKISKDLNDFNDEFISGIEALKSLLEGSQSSIKTFDQSVRKLDKQFNILNI
                     KFVKLFDKYDNQDNINKEILFDIKESSKNIYNATESQFKIRDYIKNINAGFALYERSA
                     QDLLTKLMTHENKISQNQKILLDEKFTLDDSIKNLSSIIENFSNDLQVKLDMMFENSL
                     DIQDKLDVMFNNSFMNDEVPLDSEELFKDDVNNVFNPFSEEIYEIEDKEIKVIGEDEL
                     NE"
     gene            7477..8109
                     /locus_tag="PC700_002273"
     CDS             7477..8109
                     /locus_tag="PC700_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDF2581578.1"
                     /translation="MNNKYRRTVNREFEKSSFWPTFTDLLSTVLMVVILILFSSESIS
                     GSVEQDLAKNVNASVEETFKKSKIPVKVDKSNGQVTFGEKTMFDVDSDVLKPEAKEML
                     KMFVPKYIETIYKDYGDYISKIVIEGHTDDVGSYIYNLDLSQRRAYSVAKFIVGDEIG
                     DYKYKDKVTKHIIAIGRSKAELIKNGDNSVNRDASRRVELKYEININQNK"
     gene            8438..9595
                     /locus_tag="PC700_002274"
     CDS             8438..9595
                     /locus_tag="PC700_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2581579.1"
                     /translation="MIEISIKNKKNSKIKTLILLLIVSISIYLTGCTTSDNGNNQSNN
                     NDSLSSSISSDKNVKIHFIDTGNSDAILIQDGKTFTLIDGGDNDDEGLMVDYLNNQGV
                     KDIKYLIATHSHADHLGGLDSVVKNFNIENVFVSNGSAETKSYRDFINALANKGLSPS
                     VPLENNKFYLEDSYFEVLNTNGGDTTNEQSLVLVYTNGNDKVLFTGDAEEGTEKEILP
                     KLEKVDLLKVAHHGSRSSSSQEFLDKVNPEYAVLLVGKGNSYGHPHQETMNKLKKMGV
                     KVHRSDECSDIIFESTGDGVFTSCKDGSYNKGVREDGNSGKSNSNTSKNDSFKNKQNT
                     FNSEEIVYFTPKGKSYHSIKNCSGLSRSKKILSGTIEESKKNDPCDICYGK"
     gene            complement(9743..10378)
                     /gene="lexA"
                     /locus_tag="PC700_002275"
     CDS             complement(9743..10378)
                     /gene="lexA"
                     /locus_tag="PC700_002275"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HDF2581580.1"
                     /translation="MYLDLTEKQVLILEFIKSQITLKGYPPAVREICTAVGLRSTSTV
                     HSHLNKLEKLGYIRKDPTKPRAIEVLERSKVNDVSGANQEIIELPLVGQITAGEPILA
                     QQNIEEYIPFPASLVKGSNNFVLRVKGESMINAGILDEDYVVVDKKNTALNSQIVVAL
                     INGESATVKRFFKEGNLIRLQPENDFMEPIMLNDSEVEIVGIVTGVFRVIK"
     gene            complement(10482..11609)
                     /locus_tag="PC700_002276"
     CDS             complement(10482..11609)
                     /locus_tag="PC700_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2581581.1"
                     /translation="MNNDKKIIKVHNKSDKPDNIVIKENELIEKCLLLENKLPVFMKD
                     YFIYLKGSVAVSTRLAYLEDINFFCSYLIETKELTNADCVKDITEDDFNTIKSRDINL
                     FLGDYCSRYYKNTEKNTLIFENNNRALARKKSSISTLFKFLYRNSQIDNNITDGFNPI
                     KLPKPQPDAIKRLEIDEVAKMLESVETGEGLTEKEKVYWRKTKLRDKAILALFVTYGL
                     RLNELRELNISSFNFSRGEFKIYRKRGKEVLMPINHTCEHVIKDYLQNERTRDELLND
                     EEKDALFLSLQNKRITAKAIRTLVKKYTSIPLDTTRDNGYSPHKLRATAATSLIQTGF
                     SIYDVQNLLDHDNVTTTQLYAAHKKNVKRDIVKNFEWIEDD"
BASE COUNT         4418 a         1441 c         1915 g         4007 t
ORIGIN      
        1 ttttactgat aatcttttcc ttatcactaa tcttacattt taaaatatta tttttactcg
       61 taatattatc ataagaattt gtttcttatt taaaactaat tagaattata aagtttattg
      121 ccatgttatt aataaaataa atccagttaa atcgagtgct agttaaagta ataagaaact
      181 tttacaatgg tttcctttat cgattgaaat aaaagttaat atggtataat atctaaagag
      241 agaatcttat caattagaat atgcatagaa acaagtggct taaaaggaga cgttatatgg
      301 aagagatagt aaagaaggaa caagcaagta tgaatgatat ttttttgtat cttaaagcta
      361 aatcaaactc attatataaa tttgtaatat tatataatga gtttatcaat aaacctaaag
      421 attatggaac tggggaactt ataaacatgg ttgaagtaca tatgctaaca gcgattgaag
      481 aaaatccagg aataagtcca actcagttag ctaaaatatg gaacagaact aaaggtgcaa
      541 tatctcaaac tatatctaaa ttggagaaaa agggttatat aactaaagaa aagaaagaag
      601 gaaatgcaaa aactatatta ctatatccaa ccacatcagg agtagagtta agtaatgcac
      661 ataaactata tgacaccata gatgttacaa gaacatttga atacttaatc aaggagtgta
      721 cattagagga gatagatagt ttttataaag taattgattt gtttattaat ttattagaag
      781 aatctccgca ggaaaataat gattctgaaa aagtgtagct aagattgaag ttgaataaag
      841 agaaatagat attatagaag tagtacttaa gtacacatta tataaaaata taataggtat
      901 aggattaaaa agtttagtgg tttaaagttt aaccactaaa cttttttaat gtctataatc
      961 tactattatt taatttacta aaaataaaaa aactcattat attaagatga aaatagaaaa
     1021 ataactagaa aaatactata atatatcagc ttaatacaga aaaaagttaa aaatattgag
     1081 aaaaataaaa atgaaaacgt tttatattga catagagaaa aagtagtgat accatttaaa
     1141 tatagtttag tagctaaact atatttaaat tataataact attttagggg gatgttatga
     1201 atttaaaaca attaagtgaa aaatacgaag aatatgtaat tcaaatgaga agagaatttc
     1261 ataagtatcc agaattatct tttaaagaaa taagaacaac ccaaagaata agggaagaat
     1321 tacaaaaaat aggggtccct tttgtatcta ttgaaccaaa tatcgtaata gctactatac
     1381 aaggcaaaaa tgaaggtaag acaattgcaa ttagaggaga tatagatgcg cttccaatgc
     1441 aagaagattc agatgttgaa tacaaatcag aatatgatgg aataatgcat tcttgtggac
     1501 atgatgctca tggagctatg ttactaggac ttgcacatat gctaaatgat ataagaaaag
     1561 atttaaaagg tagagtatta ttagtttttc aagcagctga agaagttggt ggcggacaca
     1621 aggagataat acagtacttt aaggaaaatg gaggaccaga tagattaata gctactcatg
     1681 tttggtccga tattgaagct ggtaaaattt ctgttgaacc aggacctcgt atggctggtg
     1741 ggtctggttg gagaatagac ataacaggta gaggaggtca tggttctaga ccagatcaaa
     1801 gcatagatcc tataaagcca gcttgtgata tagttttaaa agtatcttct ataccagtta
     1861 atcatataag tgtactagaa caatgtgttg tacatgcatg tgcaatacat ggaggaacta
     1921 caataggaaa tgttattcca aacaatgcag aggtaatagg tggatataga tattttactg
     1981 aagaatcagg caaaaaagtg tttgacatta taaagcaaat gagtaatggt gttggacaag
     2041 cttatggagt ggatgtgaag gttactcata ctggaggcat aggacctgtt atcaatgatg
     2101 aagaagcagt taagatggct aaagaaatag tttctaatat agatggattg gagttagatt
     2161 cttatgagcc aatatgtgca tcagattgtt atggtgaaat attaaaagag tattctggtt
     2221 tttattgtta tctaggggta ggtaacaaag aaaaaggaat aatatatgca caacaccatc
     2281 ctaagtacaa tatagatgaa gatacattaa agttaggttg cgaatttatg gctaaatatg
     2341 cagttgaatt tttaaataaa aataataact aaaataaatc gaattttata aatggggggg
     2401 atgaaaatgc aaggtgcatt aaaggaaaat aagaaaaaaa gtggtggagg atttttagga
     2461 tggattgaaa gagttgggaa taaaatgcca catcctctag cattatttac atatattaca
     2521 ttgatagtag tagcattatc tgcaatagca agctttttag gtttttcagc aacaagtcct
     2581 gctacaggaa agttaataaa agttatgaac ttaataagtg caaaaggttt agtactattt
     2641 atgcaagagt ttgttaagaa cttccaaaac ttccctgtgc ttggggtagt tttagttatg
     2701 gcagttgcca caggagtttg tgaaaagaca ggattttttt caactgccat aaaaatgagt
     2761 ttatcaaaag ttaaggggaa tgcggttgta tttataatag cttttatagg agtatttgct
     2821 aatcaagctg gtgatgctgc ttttgtatta gtaccaactc ttgcaggagc aatattttat
     2881 ggattaaata gaaatccatt agctggtata ttctgtggat ttgcatcagt tggtggtgga
     2941 tttgcaacag ctgtaattcc tgggggatgg gacgttacgc taacgccaat aactgtatca
     3001 gctgctcaaa ctattcaacc tgcatttgat atgacattat tagctagtta ttatgcacta
     3061 tttgtatctg cgtttatagt agctacagta tcaacaatag taacggttaa atttatagaa
     3121 cctaaattag gtaagtatac tggtaaacca gaaggtatag atgataatca aggttttgag
     3181 gtcacaaaag aacaagcaaa agcagcaaag ttagcaggtt ttacagtttt agcttatgta
     3241 gcgttattaa ttgctttgtg tataccagct aatagttttt taagaagtcc agaaggttct
     3301 ttaatatttg gagcaccact tatgtcttgc ttacagtttt ttatagtaat actattcttc
     3361 ttgccaggga ttgtgtatgg aatgagggtt ggtaaaatca agactgtaaa agacttaaat
     3421 gatatattat gtcaatcaat ggcagcaatg gcaccgttta tcgtacttgc aatagttata
     3481 ggtcaattct taacgctatt ctcagtgtct aatttagcac aggtactagc tataaaaggt
     3541 ggagcattac tcaactcatt gccactacca ccacaagtta taatactgct attcttagta
     3601 ctagtagcgt ttataaatat atttgttata agcggaagta caaaatggat gatatttgga
     3661 ccagtatttg ttccgatgtt aatgcaatta aacatacatc cagcatttac tcagtttgta
     3721 tatagattag gagattctgt aactaatcat ttatcaccat taaatgcatt ctttataata
     3781 cttttagcaa cagtccaaaa atacgataaa aatgcaggta tgggaactat attctcagct
     3841 atgatacctt acactatagg ttactttgta gtacttgcag ttttagtagt agtttggatg
     3901 acaataggta taccagtagg aataggtgga caagtttggt tataaaacat atttaataat
     3961 tttgggagga aagaaaatgg aaacaactga aataagatac aattttgatg aagtaataga
     4021 tagaagtgga acaaactgtc ttaaatatga tcttaagagt caatttatgc cagaatctcc
     4081 agaagatgca ctaccactat gggtagcaga catggatttt gcatgtgctc ctgagataat
     4141 taatgcaatg catgaaagaa tagacagaaa aatatttgga tatagctctc aacaaactga
     4201 tgattattat gaagctgtat gtggatggtt taagagaaga tttgattgga aaatagaaaa
     4261 agagaatata ttctttagcc caggggttgt gcctgcgtta ggatttttca tacaaatatt
     4321 aacacaaaag ggtgatggta tcataataga aaagcctgta tactatcctt ttgaagataa
     4381 aataactaat aatgaaagaa aagtagttaa tagtccttta aagtttgaag atggtcatta
     4441 ttctatggat tatgatggat ttgaagaatt agctaaagat gaaaataata aggtgtttat
     4501 attatgtagt cctcataatc cagttggtag agtatggaaa aaagaagaac ttagaaaaat
     4561 agtagacata tgtaagaaat atgatttgtg gataatatct gatgaaatac attgtgatat
     4621 tattcgtaaa agtgaaaagc atataccatt agaagtagca tgtcctgatt ataaagatag
     4681 aataataact tgtacagctc caagtaagag ttttaactta gctggtatgc aattatcaaa
     4741 tatagtaatc aataataagg aattacaaga aaaatggatg cttgaaacag attctaaatt
     4801 agctatattc ctacctaatc cattcgcaat agttgctact aaagttgctt ataatgattg
     4861 tgaagattgg atgaatcaag taaatgagta tatagatggt aatattaaat atgtagaaga
     4921 ttttgttaat gagtatatgc ctaaagtaaa gataataaaa tctcaaggaa cttacttaat
     4981 ttggttagac tttagagggt atggattaga tgcaaaacag cttgaatatg taatgcttaa
     5041 caaggcgaag gtattgctag atgaaggata catattcgga gaagaaggag caggatttga
     5101 aagaataaat gttgcttcgc ctagaacaat tatagaagag tgtatgaata gaataaaatg
     5161 tgcatttgaa ggattataat agtaatatct aaataatata tttgtgtatt ttaaaaaaag
     5221 actatgttaa aaaaatattt caaagcatag tcttttaaag ttttatgatg agtttaaaaa
     5281 atttcaaaac gttactatta ttgataatct tcacttatat gtttatatta gaaataattc
     5341 cacaatatgc tcattttcta aagttgcact tttatacacc ttgcttcacc aatatcatgt
     5401 gtaaccaaaa tagtagactt attctcattc ttaataatac tataaacatc atcacaaact
     5461 aataatctag tctgataatc aagagctgaa aaaggttcat ctaaaagtag aatatctgga
     5521 tttacagaaa gtgttctaat aagcgcaact ctttgtctca tacctccaga taattctttt
     5581 ggatacatac ttctaaaatc ccataatcca taagtcttta aaaggctttc aactctatct
     5641 aaagattttt tatctttttt gccttgaatt tccaatccaa tagttatatt atccattata
     5701 tttctccatt caagcaaatt atctttttga aacatatatc caatatttcc agtaatcttt
     5761 atatcaccac tagtcggttt taaaagatta gtaagaatat ttaatattgt agatttacca
     5821 cttccagaag gaccaagcaa agttaatatt tctccttctt tcaagttaaa attgacatcc
     5881 tttaatactt caagctcgcc ttccttagtg tagaagttca tatttatatt tttaacttct
     5941 actatgttgt cattcattat gccaccccct ataactttta gctcgtatat attatgttat
     6001 taacaatatg acctatttgt accatttttc ataaatataa tataaatatt ttgtttattt
     6061 acgtattttc ttagtaactt gtacgtataa attataataa ataaattata aggtagggga
     6121 taaatttgag taacataata acgaatttaa tagagtcagt atttactaat ccaataacta
     6181 tattgttttt acttgcaata actgtgtatt ctgtttttaa aataatagaa aataacagag
     6241 tatattcatt tgtaagttca aaattagacg aaatcaatag agattataag acaagtgagt
     6301 tttataaaaa ggtaagggat gactactata catatagtaa agaaaatcct tatgcagacg
     6361 taaatataac ttcatttata gaggaagttg tatctgattt aaagcataat aatttgccat
     6421 tgttagaaaa aattagaagt ataaaaaatt catcttcaat aagtatatta cttggggttt
     6481 taggtacgtt tgttggtctt tctacaatgc ttttatgtgt tgatactaaa gacattataa
     6541 attcacttcc ttcaactatt agtagtatgc aaactgcatt tacaactagt atatttggag
     6601 ttgtattttc tctaattata ggttacttta ctaagataaa agattgtgaa catgttttga
     6661 tacaaataat gttaaaatct gaaaatttac taacatctga aataactcat tttaagtcag
     6721 aaagaatgga tttaaaagta gaagaggtaa aaaatacaat aaaacaaata agtaagtcta
     6781 tagaagctat tgaaaaattt gataaaatat ctaaagattt aaatgacttt aatgatgaat
     6841 ttatcagtgg tatagaagct ttaaaaagcc ttttagaagg ttctcaaagt tctattaaga
     6901 catttgacca gagcgtaaga aaattagata aacagtttaa tattttaaat ataaaatttg
     6961 taaagctttt tgataaatat gataatcaag ataatataaa taaagagatt ttatttgata
     7021 taaaagaatc ttcaaaaaat atatacaatg ccactgaaag ccaattcaaa attagagatt
     7081 atataaaaaa tataaatgca ggatttgctc tatatgaaag aagtgctcaa gatttattaa
     7141 ctaaattaat gacccatgaa aataaaatct ctcaaaatca aaaaatcctt ttagatgaaa
     7201 agtttacctt agatgactca ataaaaaatc tctcaagtat tattgaaaac ttttctaatg
     7261 acctacaagt taaactggat atgatgtttg aaaattcttt agatattcaa gataagttag
     7321 atgttatgtt taataactct tttatgaatg atgaagttcc tcttgatagt gaggaattat
     7381 ttaaggatga tgttaataat gtatttaatc cttttagtga agaaatttat gagatagagg
     7441 ataaagaaat aaaggtcata ggtgaggatg agttaaatga ataataaata tagaagaact
     7501 gttaacagag aatttgaaaa gagtagcttt tggccaacat ttacagattt attatctact
     7561 gtattgatgg ttgtgattct tatcttattt agctcagaaa gtatttcagg ttctgttgaa
     7621 caagatttag ctaagaatgt caatgcttct gttgaagaaa cttttaaaaa aagcaaaata
     7681 cctgtgaagg tagataaatc taatgggcaa gtaacttttg gtgaaaaaac catgtttgat
     7741 gtagatagtg atgttttaaa accagaggct aaagaaatgc taaaaatgtt tgttccaaag
     7801 tacatagaga ctatttataa agattatggt gactatatat ccaaaatagt tatagaaggt
     7861 catacagatg atgtgggaag ttatatatac aacttagatt tatcgcaaag aagagcatat
     7921 agcgttgcta aatttatagt gggagatgaa ataggtgact ataaatacaa agataaagtg
     7981 actaagcata ttattgcaat aggaaggtca aaagctgaac tcatcaaaaa tggtgacaat
     8041 agcgtgaata gagatgcatc tagaagagta gaactaaaat acgaaataaa tattaatcaa
     8101 aataaataat taaattttta taaaaaaaaa gaaggcagtt ggtgtgagct ttaagccttc
     8161 tttaaggaaa aagtttattt atttttgttg atacttaaca tattatagtt tttagaagca
     8221 gtttgcaata tattaaacgt aaatggtcgt ttaaaatgca taactggata ctactgatgt
     8281 aaaatatttc attttatata atataaaaat tatacagtgt caattttcta tattattgta
     8341 ctataatcta ttatttaaaa tattatgtaa aaaaatatca taaaaacgca tatcatgtta
     8401 taattactag agtattatga atatggaggc gtagtttgtg atagaaataa gtataaaaaa
     8461 taaaaaaaat tctaaaatta aaactttgat acttttattg atagtatcta tatcgattta
     8521 tttaactggt tgtactacat cagataatgg aaataatcaa tcaaataata atgattcttt
     8581 gtctagtagt atatcaagtg ataagaatgt aaaaattcat tttattgaca ctggaaatag
     8641 tgatgcaata ttgattcaag atggtaaaac ttttacattg atagatgggg gagataatga
     8701 tgatgaggga ttaatggtag attacctaaa caatcaaggt gtaaaagata ttaaatatct
     8761 tatagctacc cattctcatg cagaccattt aggtggactt gattctgttg tcaaaaactt
     8821 taatatagaa aatgtttttg tatcgaatgg ttcagcagaa acaaagagtt atagggactt
     8881 tataaatgct ttagcaaata agggtttatc cccaagtgta ccgcttgaaa ataataagtt
     8941 ctatttagaa gattcttatt ttgaagttct aaatacaaat gggggagata ctactaatga
     9001 acaatctcta gtccttgtat atactaatgg gaatgataaa gttttattta caggtgatgc
     9061 tgaagaaggt accgaaaaag aaatactacc taagcttgag aaagtagacc ttttaaaagt
     9121 agctcatcat ggttctcgta gtagttcatc tcaagagttt ttagataaag taaatcctga
     9181 atatgcggtt cttttagtag gcaagggtaa ttcatatggt catccacatc aggagacaat
     9241 gaataaattg aaaaaaatgg gagtaaaagt acatagaagt gatgagtgtt cagatataat
     9301 atttgaatca acaggtgatg gagtatttac aagttgtaaa gatggaagtt ataataaagg
     9361 tgttagagaa gatggaaact ctggaaaatc aaacagtaat acaagtaaaa atgattcttt
     9421 taaaaataaa caaaatacat tcaattcaga agaaatagtg tattttactc caaaaggtaa
     9481 gagttatcac tctataaaaa attgtagtgg gctttcaaga agtaaaaaaa tattatcagg
     9541 cacaattgaa gaaagtaaga aaaatgaccc atgtgatata tgttatggaa aataacaaaa
     9601 aaacaaccaa tttatctata aagcttctga actttaaatc atttaaaaaa taaaataaat
     9661 gcataaaaaa aataagctat aatcaataga taatacattt catctatacg aatagcttat
     9721 ttttttaagt attttttaat agctatttaa taactctaaa tacaccagtt acaataccta
     9781 caatttcaac ttctgagtca ttgagcataa ttggttccat aaaatcattt tctggctgaa
     9841 gtcttattaa atttccttct ttaaaaaacc tcttaactgt agcagattca ccatttataa
     9901 gtgcaactac tatctgagaa tttaaggccg tatttttttt gtctactaca acgtaatctt
     9961 catctaaaat tcctgcatta atcatacttt cgccttttac tcttaataca aaattgttac
    10021 tgccctttac taaactggca gggaatggaa tgtattcctc tatgttttgt tgagctaaaa
    10081 tgggttctcc agctgttatt tgacctacta aaggaagctc tattatttct tgattagctc
    10141 cagaaacatc atttacttta ctacgttcta aaacttcaat agctcttggt ttagtagggt
    10201 cctttcttat gtatcccagt ttttcaagtt tatttagatg agagtgcaca gttgaagtag
    10261 atcttaaccc tacagcagta catatttctc ttacagcagg tggataacct tttaatgtga
    10321 tttgagactt tataaattcc aatatcaaga cttgcttttc agttagatct aaatacataa
    10381 catcactcat ttccaaacat attttgtttt tatttccata cactttatat ttttaattat
    10441 aacataatcc atttatatct gcaaacattt gttcctgatt tttaatcgtc ctcaatccat
    10501 tcaaaattct tgactatatc acgttttaca tttttcttat gagctgcata aagctgagta
    10561 gttgtgacat tatcatggtc aagtaagttt tgaacatcat atatagaaaa tcctgtttgg
    10621 attagagaag ttgcagccgt tgctcttaat ttatggggac tatatccatt gtctctagta
    10681 gtatcaagtg gaatagatgt atattttttt accagtgttc ttatagcctt tgctgtaatt
    10741 ctcttatttt gaagagacaa aaataaagca tctttttctt catcatttaa tagttcatct
    10801 cttgttcttt cgttttgcaa ataatctttt attacatgtt cacaagtatg atttataggc
    10861 attaaaactt ctttcccacg ttttctatat attttaaatt cacctcttga aaaattaaaa
    10921 gaggagatat tgagttctct aagctcattt aatctaagcc cgtatgtaac aaataatgct
    10981 agtatagctt tgtcacgtaa tttagttttt ctccaatata ccttttcttt ctctgtaagc
    11041 ccttcacctg tttctactga ttcaagcatc ttagcaactt catctatctc aaggcgctta
    11101 atagcatctg gctgaggttt aggaagtttt attggattga atccatcagt tatattatta
    11161 tctatctgag agtttctgta taagaattta aatagagtag agatggatga tttttttctt
    11221 gctaacgctc tattgttatt ttcaaaaatc aatgtgtttt tttctgtatt tttgtaatat
    11281 ctactgcaat aatcacctaa gaataaattt atatctcttg attttattgt attaaaatca
    11341 tcttctgtaa tatcttttac acaatctgca tttgttaatt ctttggtttc aatcaaatat
    11401 gaacaaaaaa aattaatatc ttctaaataa gcaagtcgtg tagaaactgc aacagaacct
    11461 tttaaatata taaaataatc cttcataaat acaggaagtt tattttccaa aagtagacat
    11521 ttttctatga gttcattttc tttgataaca atattatcag gtttatctga tttattatgt
    11581 acttttataa ttttcttatc attattcaaa attaaacctc ctaaaactaa aatataaatc
    11641 ttttatatta ttttttgata tttataccaa gtgagtataa atatttaatt taatcatttt
    11701 ttacattttt aagagattag cagtgcttta gatgaaatta tcactaacta ttccctaaat
    11761 ccacatttaa agactagttg a
//
LOCUS       DAMJWP010000105        11695 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.109, whole genome shotgun sequence.
ACCESSION   DAMJWP010000105 DAMJWP010000000
VERSION     DAMJWP010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11695
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.109"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            22..1722
                     /locus_tag="PC700_002277"
     CDS             22..1722
                     /locus_tag="PC700_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581582.1"
                     /translation="MKRYLKTYGVALIIVIIYAFIKLPVLRLDFTSLFSVGIIFFVVA
                     GILDMMLDRNERASKMAKYNFSIAIALLVYIVVVPFITSTPVLHAKSYRELLGKVTES
                     KFTDDISPVSVNDIRLVDEDMAMKLGDKKLGEVPAIGSVSKLGKFHIQNVDGELYWVA
                     PLVHRDIIKWITSLSGTSGYVKVSASNPQDVQLVQEIDGKPIKIVYQPEAYLHQDLQR
                     HLYIHGIVNVGMTDFTLEINDEGRPYWVVSLYEHKIGYGGANATGIATVDAETGKINV
                     YDVKNTPKWVDRIQPQSFVTDQIKDWGVYVNGFLNSVISEKGVLVPTEGTSLVYGNDN
                     RSYWYTGITSSGGDESTIGFMLVDSRTKEARLYKQPGATETAAMKSAEGNVQEKNYEA
                     TFPVMYNILGQPTYVSSLKDKAGLVKRVAFVSVEDYNVLGIGEDKNEALRNYKDALES
                     KGNDLKLDNDLKDEVLEGTVTRISPDVRGGNTNYYVTLDSNKDIIFRATSKVSSELPL
                     TQVGDKVKISYSKEESGVIEMSEFDNLNIANFEDKKAKEKTGTDENTDSKDSENQGNV
                     "
     gene            1892..2509
                     /locus_tag="PC700_002278"
     CDS             1892..2509
                     /locus_tag="PC700_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581583.1"
                     /translation="MTNMEKEILLLISRVRCVTESQFRKIYGDQKRYNKKNFKKTLRK
                     MCSEYTLKKYPCDVDYYNYKDLSYVYYLNGSKLFKGDDLIKALVGSELVVRINTAGCD
                     VKRFYRNVSVDDVKYDLFIEYTDQFGNIKQVLVDVDLNNEIDISKYENLSKKIDKSTI
                     PFFKVPNVLVVTPDNLSELNDLERYDNEYSINFIDLGLNKLFNCI"
     gene            2870..4063
                     /locus_tag="PC700_002279"
     CDS             2870..4063
                     /locus_tag="PC700_002279"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDF2581584.1"
                     /translation="MSIIVQKYGGSSVADTEKIKSIAENIIERRKENPQMVIVVSAMG
                     KSTDEYITLAKELSNEPSKRELDALMSTGEMISASLLSIALNALGCKAISYNAYQLNI
                     KTSGLHGKSQIDDINVNRIKNSLDEGNVVIVTGFQGINEDGDVTTLGRGGSDTSAVAL
                     AVKLNGKCEIYTDVDGIYFTDPRKYSKASKLDEIEYEEMLELASLGAQVMHSRSIELA
                     QKYGVEIYVGRTCGTEKGTYIRGGKDMKLEDKVITGLATSDDDSSITIKDFKAENISS
                     LFEDIATIGISVDMISQTAPILDKISVSFTVPKEELGECKKIVSKYTDEEHVVIDNNI
                     TKFSLVGLGMKNTSGVAAKVFKIFNENGIMIKLITTSEIRITCAINSDDKQVAIEKIA
                     EVFNI"
     gene            4081..5373
                     /gene="lysA"
                     /locus_tag="PC700_002280"
     CDS             4081..5373
                     /gene="lysA"
                     /locus_tag="PC700_002280"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDF2581585.1"
                     /translation="MKLHGTMTVNNDELYIGGVSCLELSKKYQTPLYVFDEALVRQNC
                     REYIEYFKAREGKNKVAYAGKAFLPLYMCNLINEEQMCLDVVSGGELYTAYKSNFPME
                     KVFFHGNNKTKEEINMGLDLGVGKFVVDNFYELDLLEALCEDKGKTQEIYFRITPGIE
                     AHTHDYIKTGQIDSKFGFALVNGDLFRAIEKLSEYKNIKLVGLHAHIGSQIFDVEPYI
                     DTVDIMMNLVKEIKEKFNIQLTEIDLGGGVGVYYSEGDKPKTIKEFCETIITKAETSC
                     KELGIEVPTLVIEPGRSIVANAGSTLYTVGSIKDIKDVRVYVSVDGGMTDNVRPSLYQ
                     ASYECSIVNKINHEAMNHVTIAGKCCESGDILIGDINIMDIKSGDILITTSTGAYGYS
                     MSSNYNKIPRAAVVSVFDGNDKIICKRQSYEDLLALEI"
     gene            5590..6249
                     /locus_tag="PC700_002281"
     CDS             5590..6249
                     /locus_tag="PC700_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581586.1"
                     /translation="MRGRVILLISILSIFLVGCSFNKKDEINLVEQGKTYLEKHNYKK
                     AMESLSSALEEDSTNENARAMYMQAMRMSNMTEFEELKNYEGAIKELKLIEKIKNGSS
                     VIKEEATKKKEELTKLEKEQNEAEEQRKKKAKDTAGEDRYRVESDARKSSYSGSSKNN
                     KYSSKNNDKKSDSNKSDNNNSSSQNQEKPNNSNPTPAPTPQDTTGGSDSGGNSQNSNN
                     Q"
     gene            complement(6404..7489)
                     /locus_tag="PC700_002282"
     CDS             complement(6404..7489)
                     /locus_tag="PC700_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDF2581587.1"
                     /translation="MIIILKENIKYYLIVVFIGILFFKFINTPSNFISSIEGFLKFFS
                     PFLLAILLALLLNPLVMLFEVKFKAHRLLAIFLSYIFIGFILAFAIRLLIPSIANTLN
                     RLINEMPMYTDYIDSFIEKNMSNIDFLKTLIPHIQHSLDNLLKEASNFVGKIPKNFLI
                     YTLSISSMLFNMTMGFILSIYIIYDKEKIALGFKRFLYSSTARNKADNIIEFFRTTHD
                     IFYDYLLGRILDSLIIGIIAFLGFQFVIRIENALFLASIIFLGNIIPYFGPFIGAIPP
                     IAMTILYSPQKTIWVIAFLFILQQLDGNFIEPKVMGNQVGIGAIWVISAILVGQSLFG
                     FIGVFLSVPIAAVVKTYVDKYIDNRLQ"
     gene            7680..8396
                     /locus_tag="PC700_002283"
     CDS             7680..8396
                     /locus_tag="PC700_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDF2581588.1"
                     /translation="MRINKYIASCGIASRRKSEEIILGGRIKVNGSVVKELSFNIDEE
                     KDIVEFDNKKVKPSENYIYIVLNKPEGYITTVKDQFNRPSVIDILKDVRERVYPIGRL
                     DYETSGLLILTNDGDLTYKLTHPKHEIDKTYVASVKGIISDDEIRQFETGLKIEDYTT
                     APAKIRVTKENKEKNYSVCEITIHEGRNRQVRKMCKAINHPVLNLRRISVGKIVLKDT
                     KVGEYRYLTEDEIKYLKSIK"
     gene            8454..9032
                     /locus_tag="PC700_002284"
     CDS             8454..9032
                     /locus_tag="PC700_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF2581589.1"
                     /translation="MAYAKYLTKITDINKFYLENIINEGDIVIDATMGNGYDTRYLAE
                     KVGEKGFVYAFDIQEEAIKSTRKKLEKEGYTDRVKLIFDGHEKMNQYIKEEVSCVLFN
                     LGYLPRAKHSIITKPDTTLKAIKLSLEMLKENGVISIAIYTGHDGGQEEKDCIYNLVN
                     NLNQDEFNVLESKFLNQVNNPPQLILIEKKKA"
     gene            9426..10298
                     /locus_tag="PC700_002285"
     CDS             9426..10298
                     /locus_tag="PC700_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2581590.1"
                     /translation="MEETNEMKDSKHLISNIQSQYTRLSKGQKLIAQYILNNYDKVAF
                     MTACKLGETVGVSESTVVRFANALGYSGYPKLQAALQELIKNKLTTVQRVEMAHDYSD
                     DFAILNKVLKSDIDNIRSTLEEIDERAFKEASNKLLRARKIYILGMRSSFVVAQYLGF
                     YLDIILDNVHIIRMDMGDAFEQIVRINEEDVIVAISFPRYSKKSYQIVNYAKEKGAHV
                     ISLTDSLFAPVASLADNTLLVKSNMASFVDSLVPALSISNALAISVGMKEKEDIKQHF
                     DDLEQIWKRYSVYE"
     repeat_region   11129..11687
                     /inference="COORDINATES: alignment:crt:1.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=11129..11157
                     /rpt_unit_seq="gttttatattaactatatggaatgtaaag"
BASE COUNT         4779 a         1186 c         2046 g         3684 t
ORIGIN      
        1 ttttttagag aggagtatta aatgaaacga tatcttaaaa catatggagt tgcattaata
       61 attgtaataa tttatgcatt tattaaatta cctgtactaa gattagattt tacatcttta
      121 ttttcagtag gaataatttt ctttgttgta gcaggaatac tagatatgat gttagataga
      181 aatgaaagag catctaaaat ggctaaatat aatttttcaa tagcaatagc tttgcttgtg
      241 tatattgtag tagttccctt tataacttct acacctgtac ttcatgcaaa atcatataga
      301 gaactattgg gaaaagtaac tgaaagtaaa tttactgatg acattagccc tgtcagtgta
      361 aatgacattc gtcttgtaga tgaagatatg gcaatgaaat taggtgacaa aaagcttgga
      421 gaagttcctg caatagggag tgtatccaaa ttaggaaaat ttcatataca aaatgtagat
      481 ggagagttgt actgggtagc gcctcttgtt cacagagaca taattaaatg gataacatct
      541 ctaagtggta caagtggtta tgtaaaagta tcagctagta atcctcaaga cgtccaatta
      601 gttcaagaaa tagatggaaa gccaataaag atagtttacc aaccagaagc ctacttacat
      661 caggatttac aaagacattt atacatacat ggtattgtaa atgttggtat gacagatttc
      721 actcttgaaa taaatgatga aggaagacct tattgggttg taagtttata tgaacataaa
      781 attggctatg gaggagcaaa tgcaacagga atagccactg ttgatgcaga aactggaaaa
      841 ataaatgttt atgatgttaa aaacacacct aaatgggtgg atagaataca accacaaagc
      901 tttgtaacag accagattaa agactggggt gtttatgtta atggattttt aaactctgtt
      961 atatcagaaa agggagtttt agtaccaaca gaaggtacat cattagttta tggcaatgat
     1021 aacaggtctt attggtatac aggtataaca tcttcaggtg gagatgaatc tacaattgga
     1081 tttatgttag ttgattctag aacaaaagaa gctagattat ataaacaacc aggagctact
     1141 gaaacggctg caatgaaatc agctgaaggt aatgttcaag aaaaaaatta tgaagcgacg
     1201 ttccctgtaa tgtataatat attgggtcag ccaacatatg tatcatcatt aaaagataag
     1261 gctggtcttg ttaagagagt tgcatttgta tctgttgaag attataatgt acttggaata
     1321 ggtgaagata agaatgaagc attaagaaac tataaggatg cattagaatc taagggtaat
     1381 gatttaaaac ttgataatga tttaaaagat gaggtattag aaggcacagt gacaagaata
     1441 agcccagatg tgcgaggtgg taacactaat tattatgtaa ctttagattc aaataaagat
     1501 ataatattta gggctacttc aaaagtatct agcgaattgc cattgactca ggttggtgat
     1561 aaagtcaaaa tatcgtattc aaaagaagaa tctggtgtaa tagaaatgtc agaatttgat
     1621 aacctaaata ttgcaaactt tgaagataaa aaagcaaaag aaaaaacagg aactgatgaa
     1681 aatacagatt ccaaagattc tgaaaatcaa ggtaatgtat aatttaaata aaaagattga
     1741 taatagtatt taacatagtg atagctaatt tttatagaat ttaattctat aaaaattagc
     1801 tattttattt taattctttg taaatttaat gtcataaaat taatataata ttagtacaac
     1861 tttgcaaata atacttcaag gagaatgtac tatgacgaat atggaaaaag agatattact
     1921 tttaattagc agggttagat gtgttacaga gtctcaattt agaaaaattt atggagacca
     1981 aaaaagatac aataaaaaaa attttaaaaa aactttgaga aaaatgtgta gtgaatatac
     2041 actaaaaaaa tatccttgtg atgtggatta ttataattat aaagacctct cttatgtata
     2101 ttatttaaac ggtagtaaac tttttaaggg agatgattta ataaaggctc tagtaggatc
     2161 agaattagtt gttagaatca atactgcagg ttgtgatgta aaaagatttt accgaaatgt
     2221 tagtgtagat gatgtgaaat acgatttatt tattgaatat acagaccaat ttggtaatat
     2281 aaagcaagtt ttagttgatg ttgacttaaa taatgaaata gacatttcta aatacgaaaa
     2341 tctttctaaa aaaatagata aatctacaat tccatttttt aaagtaccaa acgttttggt
     2401 agttacacca gataatctat ccgaattaaa tgatttagag agatatgata atgagtatag
     2461 cataaatttt atagatttag gattaaataa actatttaat tgtatataag ctaactttag
     2521 aaaagaaaac tatcgatttg ccacattttg taaaataatt gttgcaatat tatatagaat
     2581 atgataaaac tatattaata aattaattaa aaataatgag cctagggtag aggtagctgt
     2641 attcaatagt attcaatagg agctggtaag cattgataat tgatgaaagg gtatatagcc
     2701 gaagaaattt tgttgacgga caaattttct ggacttatgc agaatatgca taaggctgtc
     2761 acttttaaag tggtgagcta caaggttact tggtctatga aatttacacg tccaaagtat
     2821 acctttgggc gtttttattt atcaaaattt agcttgaggg ggagaaagca tgagtataat
     2881 tgttcaaaaa tatggtggaa gttcagtagc agatactgaa aaaataaaat caatagcaga
     2941 aaatataata gaacgaagaa aagaaaatcc acaaatggta atagttgtgt ctgccatggg
     3001 aaaatccact gatgaatata taactttagc aaaggaattg agtaatgaac ctagtaagag
     3061 ggaattagat gctttaatgt ctactggtga aatgatttca gcatctttat tatcaatagc
     3121 attaaatgca cttggatgta aagctataag ttataatgct tatcagttaa atataaaaac
     3181 gagtggtctt catggaaagt ctcaaattga tgatataaac gtaaacagaa ttaaaaatag
     3241 tttagatgaa ggaaatgtcg tgatagtaac tggatttcag ggaatcaacg aagatggtga
     3301 tgtaaccact cttggaagag gtggttcaga tacgtctgct gtagctcttg cggttaaatt
     3361 aaatggaaaa tgtgagatat atacagatgt tgatggaatt tattttacag acccaagaaa
     3421 atattcaaag gctagtaaac ttgatgagat tgaatatgaa gaaatgcttg agttagcgag
     3481 tttaggagca caagtaatgc attcaagaag tatagagctt gcacaaaaat atggtgttga
     3541 aatctatgtc ggacgtacat gtggtacaga aaaaggaaca tatataagag ggggaaaaga
     3601 catgaaatta gaggataaag taataacagg attagctact agtgatgatg acagttctat
     3661 aacaataaaa gattttaaag ctgaaaatat atctagctta tttgaagata tagcaactat
     3721 aggtataagt gtagatatga taagccaaac tgcacctatt ttagataaga taagtgtatc
     3781 atttacagtt ccaaaagaag agttgggaga atgtaaaaag atagtatcta aatatacaga
     3841 tgaagaacat gtagtgattg ataataatat cactaaattt tctttagtag gtcttggtat
     3901 gaaaaatact tctggagttg ctgctaaagt atttaagata ttcaatgaaa atggtataat
     3961 gataaaactt ataactacat cagagattcg tataacttgt gctataaatt ctgatgataa
     4021 gcaagtagct atagaaaaaa tagcagaagt atttaatata taaaatttgg ggaggctatt
     4081 atgaaacttc atggaactat gactgtaaat aatgatgaat tatatattgg aggagtgagt
     4141 tgtttagaac ttagtaaaaa atatcaaact ccactttatg tatttgatga agctttagtt
     4201 agacaaaatt gtagagaata tatagaatat tttaaggcta gagagggaaa aaataaggtt
     4261 gcttatgcag ggaaagcttt tttacctctt tacatgtgta atcttataaa tgaagaacaa
     4321 atgtgtttag atgtagtttc aggaggcgag ttatatacag cttataaatc aaattttcct
     4381 atggaaaaag ttttctttca tggaaacaat aagactaaag aagaaataaa catgggatta
     4441 gatttaggtg taggtaaatt tgtagtggat aatttttatg aattagattt gcttgaagca
     4501 ttatgtgaag ataaaggtaa aactcaagag atatacttta gaataactcc tggtatagaa
     4561 gctcatacac atgattatat aaaaactgga cagatagatt ctaaatttgg atttgcttta
     4621 gtaaatggag atttatttag agcaatagaa aaattaagtg aatataaaaa tattaaactt
     4681 gtaggtcttc acgctcatat aggttcacaa atatttgatg tagaaccata catagataca
     4741 gtggatataa tgatgaactt ggtaaaagaa ataaaagaaa aattcaacat acaactaaca
     4801 gaaattgact taggtggagg agttggcgtt tattatagcg aaggagataa acctaaaact
     4861 ataaaagagt tttgtgaaac cataatcact aaagcagaaa cttcatgtaa agagcttgga
     4921 atagaagtac ctacattggt aatagaacca ggaagatcaa tagttgcaaa tgcaggaagt
     4981 acattgtata cagtaggttc tataaaagat ataaaagatg ttagggttta tgtaagtgta
     5041 gatggaggta tgacagataa tgtaagacct tcgttgtacc aagctagtta tgagtgtagt
     5101 attgtgaata aaattaatca tgaggctatg aatcatgtaa caatagctgg aaaatgttgt
     5161 gagagtggag atatattaat tggagatata aacataatgg atattaaaag tggagatata
     5221 cttataacaa catcaactgg tgcatatgga tattcaatgt cttcaaatta taataaaata
     5281 ccaagagcag cagtagtatc agtatttgat ggaaatgata agataatttg taagagacaa
     5341 agctatgagg acttattagc tttagagata taaaatggaa aagtatataa taaaaattaa
     5401 atattaaagt ttattatgag cagatttgct aggatatgta tctctaaatt aatttttggt
     5461 ttattgtatc gttcgtagca tatctgctta ttttttgttt tactaacaaa tttcttgtaa
     5521 taaatataaa atattttaaa aactttataa ttatgataaa atttaaagta tatgacaagg
     5581 agggatttaa tgagaggaag agtaatttta ttaatatcga tattatctat atttcttgta
     5641 ggatgttcat ttaataaaaa agatgaaata aatttagtag agcaaggaaa aacatattta
     5701 gaaaaacata actataaaaa agctatggaa tcattatcct ctgcactgga agaagatagt
     5761 actaatgaga atgcaagagc tatgtatatg caagctatga gaatgtcaaa tatgacggag
     5821 tttgaagaat taaaaaatta tgagggtgca atcaaggaac ttaaacttat agagaaaatc
     5881 aaaaatggtt cctctgttat taaagaagag gcaactaaaa agaaagaaga gttaactaaa
     5941 ttagaaaaag aacaaaacga agcagaggaa caaagaaaga aaaaggcaaa agatacggct
     6001 ggagaagata ggtatagagt tgaatcagat gcaaggaaat cttcttatag tggaagtagt
     6061 aaaaataata aatatagttc taaaaataat gataaaaaat cagatagtaa taagtctgac
     6121 aataataaca gttcttctca aaatcaagaa aagccaaata atagtaatcc aacaccagca
     6181 ccaactccac aagatactac tggtggttca gatagtggtg gaaattctca aaactcaaat
     6241 aatcaataag tataaaatat atgcataaat ttaatgaaca aattatatat tataaaaaat
     6301 atctcttagc aattttttct tactaagaga tattttttga gaaataattt aataaataac
     6361 agttaaattt taaactaaaa attaaagtca cagtagagat aagctattgt aaacgattat
     6421 ctatatattt atcaacataa gtttttacaa cagcagctat tggtactgaa agaaaaactc
     6481 ctatgaaacc aaatagagat tgtcccacca aaatagcaga tattacccat atagctccta
     6541 taccaacttg attacccata acttttggtt ctataaaatt tccatctaat tgttgcaata
     6601 taaatagaaa tgcaatgacc catatagttt tttgtggact atataatata gtcatggcta
     6661 ttggagggat tgcaccaata aaaggaccaa aatagggaat tatattacct aagaatatta
     6721 ttgaagctaa aaataaagca ttttctattc tgataacaaa ttgaaatcct aaaaatgcaa
     6781 ttataccaat aataagagag tctaatattc taccaagtaa ataatcatag aaaatatcat
     6841 gagtagtcct aaaaaattct ataatgttat ctgccttgtt tctagcagta gaagaatata
     6901 gaaatctctt aaatcccaat gcaatttttt ctttgtcata tataatgtat atggatagta
     6961 taaatcccat tgtcatatta aatagcatag aagatatact aagtgtatag attaagaagt
     7021 ttttaggaat tttaccaaca aaattacttg cttcttttaa taaattatct aaactatgtt
     7081 gtatatgagg aattaaagtt tttagaaaat ctatgtttga catatttttt tctataaaac
     7141 tatctatata atcagtatac ataggcattt catttatcaa tctatttaat gtattagcta
     7201 ttgatggaat taatagccta atggcaaagg ctaaaataaa acctataaat atataagata
     7261 aaaaaatggc taaaagtcta tgtgctttga attttacctc aaaaagcatc acaagtggat
     7321 ttaaaagtaa agcaagtaaa atagctaata aaaatgggct aaaaaacttt aagaatcctt
     7381 caatactaga tataaagttt gatggagtat taataaattt aaaaaataaa attccaataa
     7441 acacgactat taaataatat tttatatttt cctttaaaat aataatcact tccttaaaat
     7501 agtgtttagt cagctattac atattaatag tatgtgaaaa gaaattacaa ttatttagga
     7561 aaattaaata atttttttgc ttgtttttaa ttaaggtcta gtatagtata ataatgtagc
     7621 aaaattaaca ttgataaaaa tattaaacac acataattta agataaggaa ggattaagta
     7681 tgagaattaa taagtatata gcttcttgtg gaatagcatc aagaagaaaa tcagaagaaa
     7741 taattcttgg aggaagaata aaggtaaatg gaagtgtagt aaaagagtta tcttttaata
     7801 ttgatgaaga aaaagacata gtagaatttg ataataaaaa agtaaaacca agtgaaaatt
     7861 atatatatat agtattaaat aaaccagagg gatatataac tacagtaaaa gaccaattca
     7921 atagacccag tgtaatagac atacttaaag atgtaaggga aagagtatat ccaataggga
     7981 gattagatta tgaaactagt ggtttattaa tacttactaa tgatggcgat ttaacatata
     8041 agcttaccca tccaaagcat gaaattgata aaacgtatgt ggcaagtgtg aagggaatta
     8101 taagtgatga tgagataaga caattcgaaa caggactaaa aatagaagat tacacaacgg
     8161 cacctgccaa aataagggta actaaagaaa ataaagaaaa aaattattca gtgtgtgaaa
     8221 taactataca tgagggtaga aatagacaag taagaaaaat gtgcaaagct ataaatcatc
     8281 cagttttaaa tttaagaagg atttctgtgg ggaaaatagt attaaaagac accaaagttg
     8341 gagaatatag atacctaaca gaagatgaaa taaaatattt aaaatcaata aaataagata
     8401 aaaataaatt gagaaaaaat gaaataaagt taatagtagg gagaaatagt attatggcat
     8461 atgcaaagta tttaactaaa attacagata taaataaatt ttatttagaa aatattataa
     8521 atgaagggga catagtaatt gatgctacta tgggaaatgg gtatgataca aggtatctag
     8581 ctgagaaggt tggagaaaag ggctttgtat atgcttttga tatacaagaa gaagcaataa
     8641 agtctacaag aaagaaacta gagaaagaag gatatacaga tagagtaaag ttgatatttg
     8701 atgggcatga aaaaatgaat caatatataa aagaagaagt ttcatgtgtt ttatttaatt
     8761 tgggttatct tccaagagcg aaacattcaa ttataaccaa accagataca actttaaagg
     8821 ctataaagtt aagtttagaa atgttaaaag aaaatggagt aataagcatt gccatatata
     8881 cagggcatga tggaggtcaa gaagaaaaag attgcatata taaccttgtc aataatttaa
     8941 atcaagatga atttaatgtt ttagaatcta agtttttaaa tcaagtcaat aacccaccac
     9001 aattgatttt aattgaaaag aaaaaagcat aataaatact aaaaaacttg acaagattct
     9061 attaagagtt aatatatata gtgtgaccta ttaaaaatac tttaaataat aagtatacaa
     9121 cagcttattc tcaaaacaaa atatacatct aaatatgcaa aataagatag ataactttta
     9181 aatgagtaaa aattgaaaaa tttaaaagaa atattggata aatattaaaa aatgcaagga
     9241 aagatggtat aataaattta atcgaaaata ctatgtgaag tataaggtac agaaaagtta
     9301 gaattttatg agtttaagaa gctatgataa aaactaagat gaaattgaga taaaaaataa
     9361 aatttttaga gaaaaagaca gtgttaataa aacaaaatag tttatataac taaaaggaga
     9421 aaaaaatgga agaaacaaat gaaatgaagg acagtaaaca tttgatatca aatatacaat
     9481 ctcaatatac gagattgagt aagggtcaaa aacttatagc acaatacatt ttaaataatt
     9541 atgataaagt tgcatttatg actgcatgta aattgggtga aaccgtagga gtaagtgagt
     9601 ctacagttgt aagatttgca aatgcattag gttatagtgg atatcctaaa ttacaagctg
     9661 cacttcaaga actcataaaa aataaactaa ctacagtaca aagagttgaa atggcacatg
     9721 attattccga tgattttgca atactaaaca aagttttaaa aagtgatata gataatataa
     9781 ggtctacact agaagaaatt gacgagagag cgtttaaaga agcttctaac aaacttttaa
     9841 gagcgagaaa aatatatata cttggaatga ggagttcttt tgtagtagct caatatttag
     9901 gtttttactt agatataata cttgacaatg ttcatataat tagaatggat atgggagatg
     9961 cttttgagca gattgtaaga ataaatgaag aagatgtaat agttgctatt agtttccctc
    10021 gttattctaa gaaatcttat caaattgtca attatgctaa agaaaaagga gcccatgtaa
    10081 tatcattaac agatagctta tttgcgccag tagcatcact tgcagataac actcttctag
    10141 ttaagagtaa tatggcttca tttgtagatt ctctagttcc agctcttagt atttcaaatg
    10201 cccttgctat atctgttgga atgaaggaaa aagaagatat taaacagcat tttgatgatt
    10261 tagagcaaat ttggaaaaga tattctgtgt atgagtaaag tttgtatatt aataagtatt
    10321 attaattatt aaaatgttca aatatagtga tgtttggata aaatatataa aatagctgac
    10381 tggcatttaa ctgacagtca gtttttttat ttaaacaata ttttgcttaa attttaagga
    10441 atatctttgt ctatttcttc taaatgagag tatttattca tagttatttt catttattat
    10501 gtttcttgtt gatgatatat ttttttatag aaaagaaaat agttatggtt ttgtatctgt
    10561 tatttttgca cttattcaat ttatttttgt gaaacctgga aagataaaac tttaatagca
    10621 gataataacc ttgagattgt aaagtaacaa agtgaaaatg tagaaagaga aaaagaaatg
    10681 gaaggaagaa aagtatatag ataaagagat ataacacgta ttttgattta actgtataag
    10741 atgaaaaatt tgatgatttt gaatagttta aaaaattgtt aatgaaaata aaagacttgt
    10801 tgagctaaag tgcgattaca taggaaggat gcaataaaaa gaaaaaggaa tcattggaat
    10861 agagaatata ataaatactt ataagaatgt agatgctttt agtttgtaaa attatcccat
    10921 ttttatttta tagtatgagt tttatgatat aataaaaata taaaagtttt gcagtgagcg
    10981 atttttgtga taaagtaggg tttaatagtt gaaatataaa gcgttgagag tgtatgataa
    11041 ctgttatcaa ttgcactatt gctcgctcac tgcaagttta ggagaattgt atatgtataa
    11101 gtattggaaa tacttaattt attttggggt tttatattaa ctatatggaa tgtaaagtag
    11161 gattggtgtg ttacgaccac acaacgctgg actgttttat attaactata tggaatgtaa
    11221 agagtgaatt tggcgaactc gatatagaga aaaaaataaa ggttttatat taactatatg
    11281 gaatgtaaag gaagttttta atgttgtcgc gactcttgct gttgcttgtt ttatattaac
    11341 tatatggaat gtaaagctgc tttggctttt ggtcgtataa tgctccttat aatagtttta
    11401 tattaactat atggaatgta aagttaaatc tagcatcagt cattagtata aaatttcctc
    11461 gttttatatt aactatatgg aatgtaaaga ataaaaaagg tatagttttc ttcttagtta
    11521 ctgccatgtt ttatattaac tatatggaat gtaaagtgtt ataatattaa aacacaacta
    11581 aaatatacag aaagttttat attaactata tggaatgtaa agtctagttt tttctcttta
    11641 gaaaaaataa aaacaactgt tttatattaa ctatatggaa tgtaaattaa aattg
//
LOCUS       DAMJWP010000106        11446 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.110, whole genome shotgun sequence.
ACCESSION   DAMJWP010000106 DAMJWP010000000
VERSION     DAMJWP010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11446
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.110"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            325..477
                     /locus_tag="PC700_002286"
     CDS             325..477
                     /locus_tag="PC700_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581591.1"
                     /translation="MKIFSFLLFVVGICFCIAGFMGHYPAFAGATLSFFATTMIAVRQ
                     VKTKKK"
     gene            complement(769..1473)
                     /locus_tag="PC700_002287"
     CDS             complement(769..1473)
                     /locus_tag="PC700_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2581592.1"
                     /translation="MEIKPLVLIVEDDKPICKFIKVSLETQNYRCVETDNGGTAISLI
                     HSLDPDLIILDLGLPDIDGIEVIGRVRACAKTNKIIVVSAREHERDKVEALDGGADDY
                     LTKPFSVTELLARVRVALRNKAQQDNINNDAPKSFEVKNLKIDYENHIVSINGEEIHL
                     TPIEYKIIELMSKYSGRVLTHKFIIDKVWGNYYESENQSLRVFMASIRRKIEKNPAQP
                     EYILTEVGVGYRMADE"
     gene            complement(1464..3071)
                     /locus_tag="PC700_002288"
     CDS             complement(1464..3071)
                     /locus_tag="PC700_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HDF2581593.1"
                     /translation="MKKERLQHEDLKEYDYDEDGYLSKKIIIKDFIKTILVMTIATGI
                     SVVFEKVGFNESNIILVYILGVLFATSITYGYFFGILSSVIGVLSFNFFFTHPLYTFL
                     AYRKDYPMTFCIMLMAAIITSTLTSRIKREVRLSHKREKMIRLLYENNKTLLKAKNKN
                     QIIEFCGSNLVNILNRTVIVTIADENNNLGKPSIYICNNDGSENIFHSVLEKEAIKEA
                     FRTGNPVGIGTKYCKNSSAYYFPIKGQESNVLGVIGVACFKIDIITENEKILLEAVST
                     QVALAIDRENLFEKNKKANLEAERERLRGNLLRSISHDLRTPLTSILGSSSTILENDD
                     VIDKETRVELLKNIYEDTSWLTHSVENILSMTRIDEGKLDIEKRPEVVDEIIAESILR
                     VKKFANSRDIKTNIPDEIIIVHVDVLLIEQVLVNLIDNAIRHTPKNSKIELTVKKESN
                     QVLFEVADNGKGIPNEDIGNIFNRFYTKNKSRNLERRGIGLGLEICKSIVEAHGGEIV
                     AFNNPSGGATFRFSIPMYDEEGKDKWK"
     gene            complement(3325..4503)
                     /locus_tag="PC700_002289"
     CDS             complement(3325..4503)
                     /locus_tag="PC700_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581594.1"
                     /translation="MEKLSDKRRVKAVSMETFVFIVLLAVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLDTVFLIMAMAVLAGALSALLSEFGVISLVNKIFKGLMRPIWGLPGASIA
                     GVVATYLSDNPAIIPFAKDKTFTQYFKKYQVPALCNLGTAFGMGLIVTTFMIAQGKEY
                     VLPAIIGNVGAIIGSIISVRIMLTFTKKYYNYDPKNDTEKQINDKGAKLEEFREIRDG
                     NVFQRTLDAILEGGKLGVEMGMAIIPGVLVVCTLVMLLTFGPSTDPATGQAVYTGAAY
                     EGIKLLPAIGDKISFIIEPLFGFTSPEAIAFPVTALGAVGAAISLVPEFIKSGAITPN
                     DIAVFTAMGMCWSGYLSTHIGMMDALDARPLAGKAILSHTIGGLCAGICAHFIFMLVG
                     "
     gene            complement(4729..5223)
                     /locus_tag="PC700_002290"
     CDS             complement(4729..5223)
                     /locus_tag="PC700_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDF2581595.1"
                     /translation="MHLFIEVIAAFFTALSFGVLFNMKGKNLILAGIGGSIAWFSYKF
                     LLNMGVTENLCFFIATVCFAIYCELCARIYMTPATTLSVCCLIILVPGYGIYNTMYSV
                     LTNNYIKAVEYGVSTLSCASSIALGLVFITTVFRKVNLYGVLTKIKENEKYKKSINKI
                     KQQK"
     gene            complement(5224..5985)
                     /locus_tag="PC700_002291"
     CDS             complement(5224..5985)
                     /locus_tag="PC700_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDF2581596.1"
                     /translation="MDIDRILNFSSNAGKAMLQSGGETYRVEETIARICQSFGIDHVD
                     VFATPTAVMASVFVDGKLHSAIKRISSRRVDLNLVHEVNSLSRAISINNLDIELCEKI
                     LDEISEDNYYSDLITIFFAGIAAATFSILFGGNVEEFIAAFTVGILTKFVIMYLSKSS
                     LNDFFVNAIGAVIIASCSILILKLGFIKTLDHLIAGAIMLLVPGLALTNALRDLLDGQ
                     LISGLAKLAEVFFVGVSIAVGMFLVLGLYFKLGGI"
     gene            6708..9542
                     /locus_tag="PC700_002292"
     CDS             6708..9542
                     /locus_tag="PC700_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2581597.1"
                     /translation="MLRKDKVLLSLHKLCENMTLKDLQSDKTGFTTIEIANHSSLDRS
                     NTSKELNNLYNEKKVLKISGKPILFLHVSIIENLIGRTLNSDEFSINNCNELLKSSKN
                     DSEDIFSTLLGHDSSLKLPIEKAKSAILYPPKGLNTLLIGPTGVGKSTFAETMYKYAV
                     ESKIFSSESKFVVFNCAEYAENANLLLSNLFGYVKGSFTGANKDKFGIVAQADGGILF
                     LDEIHRLPPEGQEMLFLLMDKGIYRRLGESDIVHKANVFIVCATTEDIHSSLLGTFLR
                     RIPVTINLPALQDRSLKERLSLIKYFFIIESKYTNATIKVHKDVIKSLLLYDCFGNIG
                     QLKSDIQLMSARAFLNYKTGLKDNIEIDLSLVPDYIYIGLLKLNENRSNLNKLSELDY
                     MLFYEFNGNNLEVEYTKELSKYNIDTSNNGFKYSTEIPYLENDIRSMFINYMEKYSTK
                     LLSISSVTHSDYTSNEIFKVINPKIYYAVEHSLKEAEKISRNKYTKQTYIALSMHISA
                     LIENINQDTYNPKYNLYNDKIFLDKDLNISKIVLSIIENDLQTSLPKDEVKFISMFLN
                     SMYLEPNNQSESIGVIILAHGNSTASSMCDVANSLLGTNHCNAIDMPLDVKVSTILDK
                     TIELVKECDEGKGVLLLTDMGSLIAFGELISEKTKIKTRSIEMVSTPIVLEAVRKSTL
                     PEMTLDELANSLSSLNPYIGRAAIDSTEINYPEKSREYVIITTCITGYGSAMKVADFI
                     NSSLKNIRKYNINLVPHNIESFKMYKNKFSNTKILAVVGSVDFNVANTPFIHIEELLN
                     GYGLDMLNKIIEGTFNANSITNIKETNLSYNSLIINFMTQNLELLDSVKLFNYVNSSL
                     FTIIELIDENLKSKFKLGYILHCSFMIERCFKNTTFPYNNITELISKNHELYSLIREA
                     FKPTEAYFNILIPDEEIAYIIDMINEYLNK"
     gene            9585..10829
                     /locus_tag="PC700_002293"
     CDS             9585..10829
                     /locus_tag="PC700_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDF2581598.1"
                     /translation="MEDEILKGKIKQLTILALIFIFITPVFAFADTPPVPNSSRAALL
                     IDQETKRILFEKNIDEKMPLASLSKMMTFLLAIEAVDKNQVKETDMVKIDKSTASVGG
                     STCKLKDGDEISLGELMQGLMLVSGNDAAIAIAKHIGKTEKNFVNMMNKKAEEIGMID
                     TYYFNPNGLPIYTDPEHKEPPIENMSTAHDIVTLGKYMYDHYENQVTRITTMQVYNDT
                     KKDFTHYNTNPLLVSVPGVDGIKTGYTDNAGYCLAFSMMVPKDAKNERNHRLIGVVLG
                     DGNKKNRISSSATLLKYGKDNFHSKKIAHKGDIIETPCVDGIDDFKITVKVDKDLYGV
                     VSDNENINPKVVFKNMNYPIHKGDIVGVAKYYNDSGKFVGSVDVKSESNIGCIPLKDK
                     IKIKVAKINKKLEIKNSVCFKV"
     gene            complement(11270..>11446)
                     /locus_tag="PC700_002294"
     CDS             complement(11270..>11446)
                     /locus_tag="PC700_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HDF2581599.1"
                     /translation="TYKTAIDEAQAILDKADATQKEIDDAVTALNTATATFEAGKVPT
                     TIALMLSRILGFMK"
BASE COUNT         4091 a         1839 c         1424 g         4092 t
ORIGIN      
        1 aaaaagacaa aaagggtaat ttttttatat caaaaaaaat gttaaattga tattatccat
       61 atataaattg atattatcca tatagatgct atatttaaca attctactta cttcccttac
      121 acttattagc atatagttat taagagtttg ttgcaaaaca acacttagca cactttaaaa
      181 agtgtggtct aataagtaat caacgaagaa gacataataa agcataaaat atgatatact
      241 tttcctgtca tcaaaactga tgattttact attaatatct gctaaaatat gcttaataaa
      301 ttaaatttta gtgggagaat agttatgaaa atatttagtt tcttgttgtt tgtagtggga
      361 atatgttttt gtattgctgg atttatggga cattacccag catttgctgg tgcaacattg
      421 agtttttttg caactacaat gatagctgta agacaagtca aaactaagaa aaaatgaaat
      481 aggcaaatcc taactttaca agttaatatt acataagtaa ttagtctaaa attggatact
      541 ttttctatta cagaaataca tcatccactt tgtattctct taaaaaaata aaatagctat
      601 gtaaattcaa gattagatag attaactctc tatccatttc attgtaaaat atacatagct
      661 atttgtattc gatttaaaat acttagattt tcttacataa caaataattt ctatctgatt
      721 attactcata ttcatttagt aatcactcag caactaatca tttagtatct attcatccgc
      781 catacggtaa cctaccccaa cctcagtcaa aatatattct ggctgagcag gattcttttc
      841 aattttcctt cttatacttg ccataaaaac tctaagcgac tgattttcac tttcatagta
      901 atttccccaa actttatcta ttataaactt atgagttaag actcttcctg aatacttaga
      961 catcaactca ataatcttat attcgattgg agttaaatgt atttcttcac cattaattga
     1021 cacaatatga ttttcataat ctatctttag gtttttaact tcaaaagact ttggagcatc
     1081 attatttata ttatcctgtt gagctttatt tctaagagcc acacgtactc tagccaaaag
     1141 ctctgttaca ctaaaaggtt tagttaaata atcgtctgct cctccatcta aagcttccac
     1201 cttatccctc tcatgctctc tcgctgaaac aacaattatt ttattagtct ttgcacatgc
     1261 cctaactcgt cctattacct caatcccatc aatatctggt agtcctaaat ccaaaattat
     1321 caaatctggg tcaagagaat gtataagtga tatagctgtt cctccattat ctgtctctac
     1381 acacctataa ttttgagttt caagagatac ttttataaat ttacaaattg gcttatcatc
     1441 ttctacaatt agaactaatg gctttatttc cacttgtctt tcccctcctc atcatacata
     1501 ggtatgctaa atctaaatgt agctcctcca gaaggattat taaatgcgac aatttctcca
     1561 ccatgagctt ctactataga tttacatatc tccaatccaa gaccaatacc tcttctttca
     1621 agatttctag acttattctt agtataaaac ctattaaaaa tattaccgat atcttcattt
     1681 ggtattcctt ttccattatc tgcaacttca aatagcactt gatttgactc tttttttaca
     1741 gttagttcta tcttagaatt tttaggtgta tgccttatag cattatcaat taaatttact
     1801 aacacttgtt ctattaataa tacatccaca tgaacgatta ttatctcatc tggaatattg
     1861 gtttttatgt ctctactatt ggcaaacttt ttgactctta atatagattc agctattatt
     1921 tcatcaacaa cttctggtcg tttttcaata tccaatttcc cttcatctat tctagtcata
     1981 ctaagtatat tctcaacaga atgagttagc caactcgtat cttcatatat atttttcaat
     2041 aattctactc tagtttcttt atctatcaca tcatcatttt caagtattgt tgaagacgaa
     2101 cccaatatac ttgtaagtgg agttcttaaa tcatgagaaa ttgacctaag caaatttcct
     2161 cttaatctct ctctctctgc ctcaagattt gcttttttat ttttctcaaa taaattttct
     2221 ctatcaattg ccaacgcaac ctgtgttgaa actgcttcta atagtatctt ttcattttct
     2281 gtaataatat ctatcttaaa acacgcaaca cctataacac caagaacatt tgattcttga
     2341 ccttttatag ggaaataata tgcactgctg tttttgcaat attttgtccc aattccaact
     2401 gggttacctg tcctaaaagc ttcttttata gcttcttttt ctaatactga atggaatata
     2461 ttctcacttc catcattgtt acaaatataa atacttggct tacctagatt attattctca
     2521 tctgcaatag tcactattac agttctattt aatatattta ctaaattaga cccacaaaat
     2581 tcaataattt gatttttatt ttttgcttta agcaacgttt tgttattctc atagagcaat
     2641 cttatcattt tttctctctt atgagaaagt ctcacttctc tttttatcct agaagttaaa
     2701 gtacttgtta taattgctgc cattagcata atacaaaaag tcataggata atcttttcta
     2761 taagctaaaa aagtatataa tggatgcgta aagaaaaaat taaatgatag cactcctatt
     2821 actgaagaaa gtattccaaa aaaatatcca taggttatac ttgtagcaaa tagtacacct
     2881 aatatgtata ctagtataat attagattca ttaaaaccta ccttttcaaa aactactgat
     2941 attccagtag caattgtcat aaccaatata gtttttatga agtccttaat aattatcttt
     3001 ttactgagat agccatcttc atcataatca tactctttta agtcttcatg ttgtagtcgt
     3061 tcctttttca tttactacaa ttcctcctta ttgttactgt ttaaattggc tctattttat
     3121 ataatacaat tttatcataa taaattcaat caatacattt aattaatgta cttaaattac
     3181 taaaaggtgc tgtcttaaaa tgaaaataaa tctctatttc aaaacagcac ctatatattt
     3241 aaattactaa tttagttttt acatacttaa atcaatagtt ttattcaatt ttataaatta
     3301 taatacttca attatggaac atttctatcc aacaagcata aatataaagt gggcacatat
     3361 acctgcacac aaaccaccta tggtatgcga aagtatcgct ttaccagcca atggtcttgc
     3421 atctaaagca tccatcatac ctatgtgtgt acttaaatat ccactccaac acatacccat
     3481 tgctgtgaat actgctatat cattaggagt tatagcgcct gattttataa actctggtac
     3541 taaagatatt gctgctccaa cagctcctaa tgcagttaca gggaatgcta ttgcctctgg
     3601 tgatgtgaat ccaaataatg gttctattat gaagctaatc ttatcaccaa tagcaggtaa
     3661 taattttata ccttcatatg cagctcctgt gtaaacagct tgacctgtag cagggtctgt
     3721 agatggtcca aaagttaata acataactaa tgtacaaact actaaaactc ctggtattat
     3781 agccataccc atttcaacac ctagcttacc accttcaagt atagcatcca atgttctttg
     3841 gaatacatta ccatctctta tttctctaaa ctcttcaagt tttgcacctt tatcatttat
     3901 ttgcttttca gtatcatttt tagggtcata attataatac tttttagtaa aagtcaacat
     3961 aattctaaca ctaataatac tacctattat tgccccaaca ttacctatta tagctggtag
     4021 tacatattct ttaccttgag ctatcataaa agttgttaca ataagtccca taccaaaggc
     4081 tgttcctaaa ttacataaag caggtacttg atacttctta aaatattgtg taaatgtttt
     4141 gtctttagca aatggtataa tagctggatt atcagataaa tatgttgcaa ctactcctgc
     4201 tatactagca ccaggtaagc cccatatagg tctcataaga cccttaaata tcttattaac
     4261 taaagatatt actccaaact ctgaaagcaa cgcactaagt gcacctgcaa gaactgccat
     4321 agccattatt aaaaatactg tgtctaataa aagcgcatgt gctgtactca ttattacttt
     4381 aaacatcatt cctgcaccca tgatgcttcc tacatatcca aatcctaccg ccaatagtac
     4441 aataaatacg aaggtttcca tacttactgc cttgactctt ctcttatccg acagcttttc
     4501 catcttgaaa tcttccccct tttaatttta aattaaggtt cctaacattg gtacaaattt
     4561 tttggtacaa actgagttct aaaacttaca ctcgtaatgc aagttgtcag ttttttgaaa
     4621 taaaaaaaaa ttacgtttat agaataagaa tatcctcttt aatttggata ctcttattct
     4681 attttatgtt attatattat catccttttg tttaaaagtc aattttttct atttttgttg
     4741 tttaatttta tttatactct ttttatattt ttcattttct tttatctttg taagcactcc
     4801 atataagttg acttttctaa atacagtagt tataaatacc aatcctaatg caattgaact
     4861 tgcacatgat aatgtactca ccccatattc tacagctttt atataattat tggtcaaaac
     4921 agaatacata gtattatata ttccataacc aggtactaat attattaaac aacaaacact
     4981 taatgtagtt gctggagtca tataaattct tgcacataac tcacaatata tcgcaaaaca
     5041 aactgtagct ataaaaaaac ataagttttc tgttactccc atatttaaaa gaaatttata
     5101 tgaaaaccaa gctatacttc cacctattcc tgctagtatt aagttttttc ctttcatatt
     5161 aaatagcact ccaaagctta aggcagtaaa aaaagctgct attacttcta taaacaaatg
     5221 cacttatatc ccccctaact tgaagtataa tccaagtacc aagaacattc ctactgctat
     5281 agatacacct acaaaaaata cttctgctaa ttttgcaaga ccagaaatca actgtccatc
     5341 taacaaatct cttaatgcat tagtcaaagc aagcccggga actaagagca ttattgctcc
     5401 tgctattaaa tggtctaacg tctttataaa tccgagcttt aaaattaaaa ttgaacaaga
     5461 agctataata actgctccaa ttgcatttac aaaaaagtca ttaagtgaag atttacttaa
     5521 atacataata acaaacttag tcagaatccc aacagtaaat gcagctataa attcttccac
     5581 atttcctcca aataatatcg aaaaagttgc tgctgcaata cctgcaaaaa atattgttat
     5641 taaatccgag taatagttat cttcacttat ttcatctaaa attttctcac ataattcaat
     5701 atctaaatta tttatactta ttgctcttga taatgagtta acttcatgaa ccaagtttag
     5761 gtctactctt cttgagctta ttcttttaat tgctgaatgt aatttcccat ctacaaatac
     5821 agatgccatt acagctgttg gagttgcaaa tacatctacg tggtctattc caaaagattg
     5881 acatattcta gctatcgttt cttcaactct ataggtttct cctccacttt gcaacatagc
     5941 tttacctgca tttgaggaaa agtttaaaat tctatctata tccacaaaac atatctccca
     6001 ttcttttata gtattattca tattttaata caagatatta tttatttaaa ctattttaaa
     6061 taattcatat ccaatattta cattttatga aaaaacttgt tttttatgct tgtcaaaatc
     6121 aaattttaag cttgtttaca ccataaaatg gctgaattaa tatttgacaa acactttcaa
     6181 ccatttatat aaccttctaa attgtataac tttttactac aaataaaatt atatctcttt
     6241 taattctttc tcttgagtat ttaagcttat agcaacttca tatttatata tgtacaatat
     6301 aaaacattat ttttatgata caaaatataa tttgtaaacg tatatatgta ttaactgtgt
     6361 atatataaat tattcttaac cttatcagtt ttttcatata tcttcatcta tatcgaccat
     6421 atattaatta cattgaccat atactaataa atgatattac atagactgaa gtatattaaa
     6481 aaacttaaat ttagttattt aacaacataa tactctaatt taatctataa aaatccatac
     6541 ctaatataat gcaactctta ttttaacacc atctattaca caaaacaaca tattaaaaag
     6601 taaaatacaa aatatccact ataaacctta ttttaatact gaatatatat tataatatat
     6661 ttaatgtagc ttaaatatag tttaaacata aagaaaggaa atggtatatg ctaagaaaag
     6721 ataaagttct attatctcta cataaattat gtgaaaatat gactttaaaa gaccttcaat
     6781 cagataaaac aggattcact acaatagaaa ttgctaacca ttcatctctt gatagaagta
     6841 ataccagtaa agaattaaat aacctatata atgaaaaaaa agttctgaaa atatctggta
     6901 agccaatttt atttctacat gttagtatta tagaaaattt aattggccgt actttgaaca
     6961 gtgatgaatt ttctataaat aattgtaatg agctacttaa atcaagtaaa aatgactcag
     7021 aagatatttt ttcaacatta cttggacatg attctagtct taaactacct atagaaaaag
     7081 ctaaatctgc tatactctat cctccaaaag gtcttaatac gttactcata ggacctactg
     7141 gtgttggaaa aagtacattt gccgaaacaa tgtacaaata tgctgtagaa agtaaaatct
     7201 tttcttctga aagcaaattc gtagtattta actgtgctga atatgcagaa aatgctaact
     7261 tattattaag taatttattt ggttatgtca aaggttcttt tactggtgct aataaggata
     7321 aattcggcat agttgcacag gctgatggag gcatattatt cttagatgaa atacacagat
     7381 taccaccaga aggtcaagag atgctttttc ttttgatgga caaaggcata tacagacgat
     7441 taggagaatc tgacattgtg cacaaagcta atgtctttat tgtgtgtgcc acaacagaag
     7501 atatacattc ttctctgctt gggacttttt taagaagaat ccctgttaca atcaacctac
     7561 ctgctttaca ggataggtct ttaaaagaaa gattatctct tattaaatac ttctttatta
     7621 tagaatcaaa atacaccaat gcaactataa aagttcacaa agatgtaatt aaatcattac
     7681 tcctttatga ttgttttggt aatataggtc agcttaaatc tgacatacaa cttatgagtg
     7741 caagagcctt tttaaattac aaaactggac ttaaggataa tatagaaata gatttatccc
     7801 ttgttcctga ttacatctat attggtcttt taaaattgaa tgaaaatcgt agtaatctaa
     7861 ataaattatc agaacttgat tatatgcttt tttatgaatt taatggtaat aacttagaag
     7921 ttgaatatac aaaagaacta tctaaataca atatagatac atcaaacaat ggatttaaat
     7981 actctacaga gattccttat ctggaaaatg atataaggag tatgtttata aattacatgg
     8041 aaaagtactc tacaaaatta ctttcgatat cttcagtaac tcattctgat tatacaagta
     8101 atgaaatttt taaagtaatc aatccaaaga tttactatgc tgttgaacat tctttaaaag
     8161 aggccgaaaa aatatcgaga aacaaataca caaaacaaac ttatattgct ctttcaatgc
     8221 atataagtgc acttatagaa aatataaatc aagacaccta taatcctaag tacaatttat
     8281 ataatgataa aattttctta gataaagatt taaatatatc taaaatagtt ttaagtataa
     8341 ttgaaaatga cttacaaaca tctttaccta aagatgaagt aaaatttata tccatgtttc
     8401 taaatagcat gtatcttgaa ccaaacaatc aatctgaaag tataggagtc ataatacttg
     8461 ctcatggaaa ttcaacagca agcagtatgt gtgatgtagc aaattcattg cttggtacaa
     8521 accattgtaa tgcaattgac atgcctcttg atgtaaaagt aagtaccata cttgataaaa
     8581 ctatagagtt ggtaaaagaa tgtgacgaag gtaagggtgt acttctttta acagatatgg
     8641 gctctcttat agcttttgga gaacttattt cagaaaaaac aaaaatcaaa actcgctcaa
     8701 ttgaaatggt atctactcca atagttttag aagcagtaag aaaaagtaca ttaccagaaa
     8761 tgactttgga tgaattagct aattccttaa gctctttaaa tccatatatt ggacgagcag
     8821 caatcgattc aacagaaata aactatcctg aaaagtcaag agagtatgta ataataacta
     8881 cttgtataac aggatatggt tcagctatga aagtagcaga ctttataaat tcttcactta
     8941 aaaatattcg taaatacaat attaatttag ttcctcataa tatagaaagt tttaaaatgt
     9001 ataagaataa atttagtaat actaaaattc tggcagttgt tggaagtgta gattttaatg
     9061 tagccaacac cccttttata cacatagagg aattactaaa tggatatggc ctagatatgc
     9121 taaacaaaat aatagaaggt acattcaatg ccaatagtat tacaaatata aaagaaacaa
     9181 atctatcata caacagttta ataataaact ttatgaccca aaacttagaa cttttggact
     9241 ctgttaaatt attcaactat gtcaattctt ccttatttac aataatagaa ttaatagatg
     9301 aaaacttaaa gagcaaattt aagctaggat atatactaca ttgctctttt atgatagaaa
     9361 gatgttttaa aaatacaact tttccatata ataacattac tgaattaatt agtaaaaatc
     9421 atgaactata tagtttaata cgtgaagcct ttaagccaac agaagcttat tttaatattt
     9481 tgataccaga cgaagaaatt gcttatataa tagacatgat taatgagtat ctaaataaat
     9541 aaaatttata gcacttattt tgtgcatatt aattaatata tgaaatggag gacgagattt
     9601 tgaaaggaaa aataaaacag ttaacaatac ttgcattaat ttttatattt ataactcctg
     9661 tatttgcatt tgcagatact ccaccagtac caaattcttc aagagcagca ctacttatag
     9721 accaggaaac taaaagaata ttatttgaaa aaaacattga tgaaaaaatg ccattagcga
     9781 gtttaagtaa aatgatgacc tttttattgg caatagaagc agttgataag aatcaagtaa
     9841 aagagactga tatggtgaaa atagacaagt ctacagcttc agtaggaggt tctacttgta
     9901 aacttaaaga tggtgatgaa atatctctag gagagcttat gcagggtcta atgctagtat
     9961 ctggaaatga tgctgctata gccatagcta aacatattgg taagactgaa aagaattttg
    10021 taaatatgat gaacaagaaa gcagaagaaa taggaatgat tgatacgtat tattttaatc
    10081 caaatgggct tcctatatat actgacccag aacataaaga acctcctatt gaaaatatgt
    10141 caactgctca cgatatagtt acacttggaa agtatatgta tgaccattat gaaaatcaag
    10201 ttacaagaat aaccactatg caagtatata atgatactaa aaaggatttt acacattata
    10261 acacaaatcc cctacttgtt tcagtacctg gtgttgatgg aataaagaca ggctatacag
    10321 ataatgctgg atattgcctt gcattttcta tgatggtacc taaagatgct aaaaacgaaa
    10381 gaaatcatag attgataggt gttgtacttg gtgatggtaa caagaaaaat agaatttctt
    10441 cttccgctac tttattgaaa tatggtaaag ataatttcca ttctaaaaag atagcacata
    10501 agggtgatat tatagagact ccttgtgttg atggtattga tgattttaag ataacagtta
    10561 aggttgataa agatttgtat ggtgttgttt cagataacga aaatatcaat cctaaggtag
    10621 tgtttaaaaa tatgaattat cctatccata agggagatat tgttggtgtt gcaaagtatt
    10681 ataatgactc tggtaaattt gttggaagtg ttgatgtaaa aagtgaaagt aatataggat
    10741 gtattccttt gaaggataag attaagatta aggttgctaa gataaataaa aaacttgaga
    10801 ttaaaaattc tgtttgtttt aaagtatagt aaagcatact attaaaagag tatattaaat
    10861 ttataaatac tccactttgt ggagtttttt tgttttttac aaaaaattaa aatagtggca
    10921 acaattgagg atgtgaggga gagttgccac tattttgtat agggtattat attatgtttt
    10981 taagctagtt ttactatctt ctatgtttat ataataactc ttgtgtgtga atttaattga
    11041 taattaatat gaaaattatg tgaagttttg atattaggtt atactttgaa ctttatctaa
    11101 cattttaaat tttatatctc taaattatct aaaattacta tatcaattaa ctttacatga
    11161 taaaaaggct gtgtataaat ttatttttaa aaattttact cacagcctta aattaaattt
    11221 tctatttaaa gtatatataa attttattaa ataatttgct ttttatttct tatttcataa
    11281 atcctagtat tcttgataac attaatgcaa ttgtagtagg tactttccct gcttcaaatg
    11341 tagctgttgc tgtgtttaat gctgttactg catcatctat ttctttttgt gttgcatctg
    11401 ctttatctaa tattgcttgt gcttcatcta ttgctgtttt atatgt
//
LOCUS       DAMJWP010000107        11425 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.111, whole genome shotgun sequence.
ACCESSION   DAMJWP010000107 DAMJWP010000000
VERSION     DAMJWP010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11425
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.111"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            60..944
                     /locus_tag="PC700_002295"
     CDS             60..944
                     /locus_tag="PC700_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDF2581600.1"
                     /translation="MKFVTFCSSNEEKIGVFNSETNSIYEINSLGLSKLYTDMNDFIE
                     NVSTGDLEKIKNNSFENAKCYKLEEVKLCSPIVRPKKDIICLGLNYKDHVNEIPDGVI
                     KNVVMPDYPIYFSKRADKIIGVDDKISLHGDLVEKLDYESELAVIIGKEGINISKEDA
                     YEYIFGYTIVNDISERALQDKHVQWFRGKSLDTHTSMGPCIVHKEEFEHPLKLDISSV
                     VNGEVRQNSNTEYFIFDIPTIINDLSRGMTLKPGDIISTGTPAGVAMGMNPQVYLKHG
                     DIVECKVEGIGVLKNIVD"
     gene            1043..2218
                     /locus_tag="PC700_002296"
     CDS             1043..2218
                     /locus_tag="PC700_002296"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HDF2581601.1"
                     /translation="MARELELKYGCNPNQKPSKIYMKNGELPIEVLNGKPGYINFLDA
                     FNSWQLVKELKEATGLPAATSFKHVSPAGAAVGVPLSDTLKQIYFVDDLELSPLACAY
                     AMARGADRMSSYGDFIALSDVCDKETATIIAREVSDGIIAPGYTDEALEILKGKRKGN
                     YNIVKIDENYTPEPIETKDVYGITFEQGRNEILINEELLKDIPTANKIFTDSAKRDLI
                     IALITLKYTQSNSVCYAKDGQVIGVGAGQQSRIHCTRLAGNKADTWYLRQHPKVLNLK
                     FKKDIGRPDRDNTIDVYLSDDYMDVLADGIWQNFFEEKPEPLTGEGKRAWLKTLTGVA
                     LGSDAFFPFGDNIERAKRSGVSFIAQPGGSIRDDNVILTCNKYNIVMAFTKNRLFHH"
     gene            2346..3200
                     /locus_tag="PC700_002297"
     CDS             2346..3200
                     /locus_tag="PC700_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581602.1"
                     /translation="MALNIRNYSLLCNQGTAKFNEDVVGINPFGAWVLDGATGLNGKN
                     LVSNESDARWYVQWWNRYLYKNINKEESLKSIINTGIIEVKKEYELRLNGFKVEKLDL
                     PSSSIVVVKFHENKVEYFLLGDCALFIKNGDSKVIKDRSICKFDNTVFDEMSKLSNLE
                     EMAFDEIRTSVMDTIVSNRLKKNTKEGYWILDFDEEAVENSIYGYIEIKKDFQLMLTS
                     DGFTSACDRYNLIKEEELIRIAEKFGIGYIHNKVRDFEDNDYKAVKVPRFKIKDDSSC
                     LYLDIYND"
     gene            3244..4479
                     /locus_tag="PC700_002298"
     CDS             3244..4479
                     /locus_tag="PC700_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDF2581603.1"
                     /translation="MKILHIITQKPNSTGSGIYLSGMIKGFEKIGHKQAVIAGIDVND
                     DVNCFPSEVSFYPVKYNCGELNFPVVGMSDSMPYESTRYKDLNIDMINRLKYQFKVNI
                     DKAMNDFKPELIICHHLYLLTAFVREIVKDIKVMSICHGTCLRQLNTIDLEKEYIIAN
                     IRKLDLIFALHENQKYDIIKTFGVSESKVVVIGSGYNDDIFYNKNYKIKDDKIKIVFA
                     GKICKSKGLIPFIKSISKLKYSKDLIEVNFAGTGSDIESYNEIVKLASKSPFKMNFLG
                     KLEQRDLAELFNRSQIFVLPSFYEGLPVVVLEALSCGTDVITTDILGVKEWIGSEINN
                     SGKIEYISLPFMEKEGIPKDEELYDFENNLYNAIDSKIQSLLNNSNEKTSVDMSKKTW
                     DGLAYRINEVILMDELCLV"
     gene            4580..5521
                     /locus_tag="PC700_002299"
     CDS             4580..5521
                     /locus_tag="PC700_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp25"
                     /protein_id="HDF2581604.1"
                     /translation="MRISKKVLALGISALLLSVSFPTSINALDKIENIQGVDKYETAG
                     LISDKQDFTTAVLINADSTMVDGIAASGLAGVNNAAILLTNKDDIPEATLQRLNRVTK
                     IYVIGGENSISKDVEKMLLMRRMQVIRIDGVDRVDTSYKIAGEIEKIKNSDKMFLVNG
                     FKDEADAVSVASVAYRDGAPIILTKDIPSAEEDTDLDPWFGVSPVPVYAIGGESTLSD
                     YIVSRYRATRIGGVDRYQTNKNVIEKFYNGAKEFYITSGDDLVYALVASPLAKNAPVV
                     LVSNKSDKSILSGASKVTAIGISDKSIIEQCLDAVKK"
     gene            complement(5750..6103)
                     /locus_tag="PC700_002300"
     CDS             complement(5750..6103)
                     /locus_tag="PC700_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDF2581605.1"
                     /translation="MDRKRELKQLYKEMKFDTGVFIIKNDITKKIYLGKSNDIKSKFN
                     SLKFQLGAGSCMIKELNNEWKKYGEKAFTFEVLELIKHDDNKTEKDYLEELDILEMIW
                     LEKLKEDNTYEIYSI"
     gene            6399..7310
                     /locus_tag="PC700_002301"
     CDS             6399..7310
                     /locus_tag="PC700_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HDF2581606.1"
                     /translation="MNKKIELIGNFASDWIRYSDYEYKITEEDELYIVPTEDAVFTMY
                     NPFDVADDIIVDIIRIGQEALQDISKETKEMKLKKEVLEFVKKYGLLGLIYSSVYNRD
                     IVGEERVLMIEKNYITKENILSTDEYVNMFIPFASDEDVYFKKYRRGIDVSKREESPK
                     FFGKRPLILDLVFSKFYTEQLDWIVSFAKDMCKHFNQICVYKNTSNLLTESVTIMSGG
                     FNPQKIGFSISQLDKPIIAWEFDSLKTAIETIYAFAITSEPSSLNKCKYCGKIYIPTN
                     SKSQYCSPSCRNCSNVKKSRSRAKKDK"
     gene            7570..8004
                     /locus_tag="PC700_002302"
     CDS             7570..8004
                     /locus_tag="PC700_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581607.1"
                     /translation="MISPIILSSINQNLKEIERNELLETNIESGDYGLALSESDVKDI
                     INSRDNTLKGYGRIELDIKVTKQLIENIYTSQYTNVDNYLEAINDMQEIFYYLKNETD
                     DKICDDEIIEILGEFYEKFSGNMDNVRGEADEFAKKFKFGEV"
     gene            8027..9562
                     /locus_tag="PC700_002303"
     CDS             8027..9562
                     /locus_tag="PC700_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581608.1"
                     /translation="MLNNLSNININENNLIKNQYSISLLKECLNCKVIDEREVYNIQQ
                     EISLILMDLIKKYTNGQSTSVKTEVAEKLLISIWYAIDAYINKLGDIEKRVVELTAKN
                     VKKVYLGGIEILEEELVRLKDFYKLMMSRKLDTNSIAYNDTLMELSSFFKSYIVKFEA
                     QDVPVSIDYPLALDDMDVKGIYYVKNYIESIDIENRFCNLFKKKDREKLFYDYGVIYK
                     IDCDVILVNLFELIINNCIFSTILGNEVINLEISKYEYEFLEKQFKNMYNNITEDKVI
                     GSEESNIIELIEVDDSFNTEVDSNLDYNDFEEINREIRINKKITLLLMEAIEVLIKRL
                     SIEDEDVIRYIKKYEKIFIESVLSSIKSNTFKSMVTVSKNKNEHLEDYIVDDESKLDD
                     ESFREIFNEILDSTSIIEKIRIIKENINAKKDFIDILESECLFGEEYLMLFASLSELE
                     LAILGSVVFYEDIRMREINILEFMLNEKTETTLWKTEYIKFMKRQSEDKIYSIEEHMN
                     KIN"
     gene            9646..11013
                     /locus_tag="PC700_002304"
     CDS             9646..11013
                     /locus_tag="PC700_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2581609.1"
                     /translation="MKEYIINNVEEIREELIDLSKKIWENPELAFEEKYASSIQKEYL
                     KSKGFKIEEVENLPTGFIASFGEGKPVIGILGEYDALPELSQCVSAERKPLVEGKAGH
                     GCGHNLLGVAGVGAVVSIKKLIEENKFNGTIKYFGCPAEEEGGGKTVMCINGCFDDVD
                     CAFTWHPFDINAPWRGGSLANLSVKFKFKGITAHAAQAPHNGRSALDAVEIMNVGANY
                     LREHVIDSIRMHYVITNGGGRPNVVPGFAESWYFIRGKKARDAEHVLDRLIKVAQGAA
                     MMTETEMEYKVTDGIYDYIPNQCLTDLVYDNMVFVGCPKNTPEEEEFAKKLCDTLTRE
                     ERLGVATSLQNDKSIVESYIHKEIVDGDKDKGLAGSTDVGDVSYVIPVAQFAMAAWPV
                     GIASHTWQSCSSAGSNIGFSAMINSAKVLACSAYDVFMNTKIIDEAKIEFDKSLDGQK
                     FKPLV"
     gene            11048..11272
                     /locus_tag="PC700_002305"
     CDS             11048..11272
                     /locus_tag="PC700_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HDF2581610.1"
                     /translation="MREKVEKVLEEKIKPVLQRDGGDVELIDVNENGVVLVRLQGACS
                     GCPGATMTIKAIIENVLVSEVPGVTQVLGV"
BASE COUNT         4557 a         1049 c         2108 g         3711 t
ORIGIN      
        1 ttatataaac taataaaaat taaataatat taatcaactt attgaatgga ggaataaaaa
       61 tgaaatttgt gactttttgt agtagcaatg aagaaaaaat aggtgtattt aattcagaaa
      121 caaatagtat atatgaaatt aatagtttag gattaagtaa attatatact gatatgaatg
      181 attttattga aaacgtatct acaggagatt tagaaaaaat aaaaaacaat tcatttgaaa
      241 acgcaaagtg ttataagtta gaagaagtta aattatgttc cccaattgta agacctaaaa
      301 aagatattat atgtttaggt ctaaactata aagaccatgt caatgaaatc ccagatggag
      361 ttataaaaaa cgtagttatg ccagattatc caatttattt ttctaagaga gcagacaaaa
      421 taattggagt ggatgataag ataagcttac atggagattt ggtagagaag ttggactatg
      481 aatcagaact tgcagtaatc ataggtaaag aaggtataaa tatatcaaaa gaagatgctt
      541 atgaatatat atttggatat acaatagtaa atgatataag tgaaagagca cttcaagata
      601 aacatgttca atggtttaga ggaaaaagct tagacacaca tacatctatg ggaccttgta
      661 tagtacataa agaagaattt gaacatcctt taaaattaga tataagtagt gtagtaaatg
      721 gagaagtaag acaaaattct aatacagagt actttatatt tgatatacct actataataa
      781 atgatttatc aagaggaatg actctaaagc caggagatat aatttcaact ggaactccag
      841 caggggttgc tatggggatg aatcctcaag tatatttaaa gcatggcgat atagttgaat
      901 gtaaagtcga aggaatagga gttttaaaaa atatagttga ttaatggata aatatgccat
      961 ataattttat tagtacaaat aatttaatta atataggatt gatcaaactt atgattaatt
     1021 tattatagag agggatgagt atatggcaag agaactagaa ttaaagtatg gctgtaatcc
     1081 aaatcaaaaa ccatcaaaga tttatatgaa gaatggagaa cttcctatag aagtattaaa
     1141 tggaaaacct ggatatataa attttttaga tgcatttaat agttggcaac ttgtgaagga
     1201 attaaaagaa gctacaggtc ttccagcagc aacttcattt aaacatgtaa gtccagcagg
     1261 agcagcagta ggggtacctt tatcagatac tttaaagcaa atatattttg ttgatgattt
     1321 agagctttct ccattagctt gtgcttatgc aatggcaaga ggagcagata gaatgtcttc
     1381 ttatggtgat tttatagcac tttcagatgt atgtgataag gagactgcaa ctattatagc
     1441 aagagaggtt tctgatggta taatagctcc aggatataca gatgaagccc ttgaaatatt
     1501 aaaaggaaaa cgtaaaggca actataatat tgtaaaaata gatgaaaatt atactcctga
     1561 gcctattgaa acaaaggatg tttatggaat tacttttgaa caaggtagaa atgaaatatt
     1621 aataaatgaa gaactattaa aagatattcc aactgcgaat aaaattttta cagatagtgc
     1681 aaaacgtgat ttaattatag ctcttattac attaaaatat acacaatcaa attctgtttg
     1741 ttatgctaaa gatggacaag taataggggt aggtgcaggt caacaatcac gtattcattg
     1801 tactagactt gcaggtaaca aagctgatac ttggtatctt cgtcaacatc ctaaagtatt
     1861 aaacttaaaa tttaaaaagg atataggtcg tccagataga gataacacta ttgatgtata
     1921 tctttctgat gattatatgg atgtgcttgc tgatggcata tggcaaaact tttttgaaga
     1981 aaaaccagag cctctaactg gagaaggaaa aagagcatgg cttaaaactt taacaggagt
     2041 agcacttggt tcagatgcat ttttcccatt tggagataat atagaacgtg caaaaagaag
     2101 tggggtaagt tttatagcac aaccaggtgg ttctattcgt gatgataatg ttatattaac
     2161 ttgtaataag tataacatag tcatggcatt tactaaaaat agattattcc atcattaaaa
     2221 tactgaaagt ataaagataa tatagattaa gtaaaaatct acaacaagtt ttgttgtaga
     2281 tttttttatt tgctgtataa ttttaaatag aactttattt ttagatattc aatttggagg
     2341 ttgatatggc tttaaatata agaaactata gcctattatg taatcaagga acagctaaat
     2401 ttaatgaaga tgtagtcggt ataaatccat ttggggcatg ggttttagat ggtgctacag
     2461 gacttaatgg aaaaaattta gtgtcaaatg aaagtgacgc aaggtggtat gttcagtggt
     2521 ggaataggta tttatataaa aatataaata aagaagaatc attaaagagt attattaata
     2581 ctggaataat agaagtaaaa aaagagtatg aactaagatt aaatgggttt aaggttgaaa
     2641 aattagattt accatctagt tctatagttg ttgtaaaatt tcatgaaaat aaagtagagt
     2701 attttttatt aggagattgt gctttgttta ttaaaaatgg agattccaaa gttataaaag
     2761 ataggagtat ttgtaaattt gataatactg tatttgatga aatgagtaaa ctttctaact
     2821 tggaagagat ggcatttgat gagattagaa ctagtgttat ggatacaata gtaagcaata
     2881 gattgaagaa aaatacgaaa gagggctatt ggatattgga ttttgatgag gaagctgtgg
     2941 aaaattctat atatggatac attgaaataa aaaaagattt tcaattgatg ctaacgagtg
     3001 atggttttac aagtgcatgt gatagatata atctaataaa agaagaagaa ttgatacgta
     3061 tagcagaaaa atttggtata ggatatatac ataataaagt aagggatttt gaagataatg
     3121 actataaagc ggttaaagtg ccaagattta aaataaaaga tgattcatct tgtttatatt
     3181 tagatatata taatgattag actgtataaa actatttaat tttagaaagg aggcaagtga
     3241 aaagtgaaaa ttcttcatat aataacacaa aaaccaaata gtacaggtag tggtatatat
     3301 ttgtctggta tgataaaagg gttcgaaaaa atcggtcata agcaagctgt aattgcaggt
     3361 atagatgtaa atgatgatgt aaattgtttt ccaagtgaag taagttttta tccagtaaag
     3421 tataattgtg gggagttaaa ttttcctgtt gtaggtatga gtgattctat gccatatgaa
     3481 agtacaagat ataaagattt aaatatagac atgataaata gattgaaata tcaatttaaa
     3541 gtaaatatag ataaggctat gaatgatttt aaaccagaac ttataatatg tcatcatttg
     3601 tacctcttaa ctgcatttgt tagggaaata gtaaaagata taaaggttat gtctatttgt
     3661 catggaactt gtttaagaca gttaaatacc attgatttag agaaagaata cataatagca
     3721 aatataagga aattagattt aatatttgct cttcatgaaa atcagaaata tgatattata
     3781 aagacttttg gagtaagtga aagtaaagta gttgtaattg gtagtggtta taatgatgat
     3841 atattttata ataaaaatta taaaataaag gatgataaaa taaaaatagt atttgcaggt
     3901 aagatatgta aatcaaaggg attgattcca tttataaaat ctatatctaa attaaaatat
     3961 agtaaagatt taatagaagt caattttgca ggtactggaa gcgatatcga atcatataat
     4021 gagatagtta aactggcaag taagtctcca tttaaaatga atttcctagg aaagttagaa
     4081 cagagagatt tagctgagct attcaacagg tcacaaatat ttgtgttacc atcattttat
     4141 gagggattac cagttgtagt tttagaagct ttatcttgtg gcacagatgt aatcactact
     4201 gatattcttg gtgttaaaga gtggattggt agtgagatta ataattctgg aaagatagaa
     4261 tatatttctc taccttttat ggagaaagaa ggtattccaa aagatgaaga gttatatgat
     4321 tttgaaaata atttgtataa tgcaatagat tctaagatac aatctttatt gaataactca
     4381 aatgagaaaa catctgtaga tatgtcaaaa aaaacgtggg atggcttagc atatagaata
     4441 aatgaagtta ttttaatgga tgaattgtgt ttagtctaaa attatttaaa ttataaaaaa
     4501 gtgtaactat ttttaaactt tttttaaatg attttatatt atactaatac taaatatatt
     4561 tgttttaggg aggtaattta tgaggattag taaaaaggta ttggcgttag gtatttcagc
     4621 attactgtta agtgttagtt ttccaacatc aataaacgca ctagataaga ttgaaaatat
     4681 tcaaggagta gataaatatg aaacagctgg gcttatttca gataagcaag attttacaac
     4741 agcagttctt atcaatgcag attctacaat ggtagatggg attgctgcaa gtggtcttgc
     4801 aggagtaaat aatgcagcta ttttattaac taataaggat gatataccag aagcaacact
     4861 tcaaagactg aatagagtta ctaagatata tgtaataggt ggagaaaatt ctataagtaa
     4921 agatgtagaa aaaatgttac ttatgagaag aatgcaagtt ataagaattg atggtgtaga
     4981 tagagtagat acaagttata aaattgcagg tgaaatagaa aaaattaaaa acagtgataa
     5041 gatgttttta gtaaatggat ttaaggatga agctgatgca gtaagtgttg catctgttgc
     5101 ttatagagat ggagctccta taatattgac aaaagatata ccttcagcag aagaagacac
     5161 agacttagac ccatggtttg gagtttctcc tgttccagta tatgctattg gaggagagtc
     5221 tacattaagt gattacatag tgagcagata tagagcaact agaattggcg gtgttgatag
     5281 atatcaaact aataaaaatg ttattgaaaa gttctataat ggagcaaaag agttctatat
     5341 aacaagtgga gacgatttag tttatgcttt agttgcatca ccacttgcaa aaaatgctcc
     5401 tgttgtttta gtttcaaata aaagtgataa gagtatatta agtggagcta gtaaagtaac
     5461 agcaataggt ataagtgata agagtattat agagcaatgt ttggatgctg taaagaagta
     5521 atggagtgtt attagtaaaa tgaatatata cattgaaaat tagtagattg ttcattttat
     5581 aatggtataa aatatatggg aagaggctat caaatggaga taagcctctt ttttattgat
     5641 aaaattatga aaatatactt tattttatat aaaaaaagta tctcatgaac taaatgtcta
     5701 ttttgatata ctatctcctt ttcttatatt atatgtatta tatgattttt tatatggaat
     5761 aaatttcata agtattatct tcctttaatt tttctaacca aatcatctct aaaatatcta
     5821 gttcttctaa atagtctttc tctgttttgt tatcatcatg ttttataagt tctaaaactt
     5881 caaatgtaaa tgctttttca ccatattttt tccattcatt atttaattct tttatcatac
     5941 atgagccagc accaagctga aatttgagag agttaaattt gctttttatg tcattacttt
     6001 ttccaaggta aatcttttta gtaatatcat tttttattat gaatacacct gtgtcaaatt
     6061 tcatttcttt gtataattgt tttaattcac gttttctatc catgatttta gatacctctc
     6121 tttataattg atatgtaata tgtaagttta aaaatatatt acatatcaat tataaagaga
     6181 ttaattttat atgtaaatag tttttataaa tttaattatg aattagtcat caataaacaa
     6241 gaaatttagc tgtagaaatc cattataaag gctattataa attttaatat gctaaaaata
     6301 taaaaagaat agttgacagt atagaaaata tggaataaac taaaagtgta atagtaatac
     6361 gaaattatat attacattga aaggtgtgaa tgattattat gaataaaaaa atagaattaa
     6421 tagggaattt tgcaagtgat tggattaggt atagtgatta tgaatataaa attactgaag
     6481 aagatgaatt atatatagtt ccaacagaag atgctgtgtt taccatgtat aacccatttg
     6541 atgtagcaga tgatataata gtagatataa ttcgcatagg acaagaggca ttacaagata
     6601 tatcaaaaga gacaaaagaa atgaaactaa agaaagaagt acttgaattt gtaaaaaagt
     6661 atggattatt gggtttaata tattcaagtg tatataatag agatatagtt ggagaagaaa
     6721 gagtactgat gattgaaaag aattatatta caaaagagaa tatattgagt acagatgaat
     6781 atgtaaacat gtttatacca tttgcaagtg atgaagatgt gtattttaaa aaatatagaa
     6841 ggggtataga tgtatcaaag agagaagaat ctccaaagtt ttttggaaag agaccattga
     6901 ttttagattt agtattttca aagttctaca ctgaacaact agattggatt gtaagttttg
     6961 ctaaagatat gtgtaagcat tttaatcaaa tttgtgtata taaaaataca tctaatctat
     7021 taactgaaag tgttactatt atgtctggag ggtttaatcc tcaaaagata ggattttcta
     7081 taagccaatt agataaacca attattgcat gggagtttga ttctttgaaa acagctattg
     7141 agactatcta tgcctttgca attacaagtg aaccttcttc tttaaataaa tgcaagtact
     7201 gtggtaaaat ttatattcct acaaattcta aatctcaata ctgttcacct tcttgtagaa
     7261 attgctcaaa tgtaaagaaa agtagaagta gagccaaaaa agataaataa aattaataaa
     7321 tatatcattg tttagatttt agtaaataac tactgaatta aaagttgtta tagaaaatta
     7381 tatatctaaa tgatggtatt tttttgttga gaaaattgaa aagtttaatt gagatagtaa
     7441 ttttgagttg acaaaaacca cttttaacac tactattata agtggttggt aaaataatat
     7501 acactaattt aaatatagca taaaaaacaa aaaaagtcaa tgaaaaaaat aaaaaaggag
     7561 aatttaaaaa tgatatcacc tattattttg agttctataa atcagaattt aaaggaaata
     7621 gaaagaaatg aactgcttga aactaatata gaatctggag attatggtct agctctttct
     7681 gaatctgatg taaaagatat aataaattcg agagataata ctttaaaggg gtatggaaga
     7741 atagaattag atataaaagt tacaaaacag ttaatagaaa atatatatac atcacaatat
     7801 acaaatgtgg acaattattt agaggctatt aatgatatgc aagaaatttt ttattattta
     7861 aaaaatgaaa ctgatgacaa gatatgtgat gatgagatta tagaaatact gggtgaattt
     7921 tatgaaaaat tttcaggcaa tatggacaat gtaagaggag aagcagacga atttgcaaag
     7981 aaatttaaat ttggagaagt ttaatatatg ataggagaga ttttgtatgt taaataattt
     8041 aagtaatatt aatataaacg aaaataattt aataaagaac caatattcca tatctctact
     8101 taaggaatgt ctaaattgta aagtgataga tgaaagagaa gtatataata tacaacaaga
     8161 aatatctcta atacttatgg atttgattaa aaaatataca aatggtcaaa gcacatcagt
     8221 aaaaacagaa gttgccgaaa aactgttgat ttcaatatgg tatgctattg atgcatatat
     8281 aaataagctt ggagatatag aaaaaagagt tgtggagttg actgctaaga atgttaaaaa
     8341 agtgtacctt ggaggcattg aaatattaga ggaagagtta gttaggctga aagattttta
     8401 taaactaatg atgagtagaa aattggatac taattcaata gcatataatg ataccttgat
     8461 ggagttatca agtttcttta aaagttatat tgtaaaattt gaagctcagg acgttccagt
     8521 aagtatagat tatccattgg cactagatga tatggatgtt aagggtattt attatgtgaa
     8581 gaattatatt gagagtatag atatagaaaa tagattttgt aacctattta agaaaaaaga
     8641 tagagaaaaa ttattttatg actatggggt gatttataaa atagattgtg atgttatact
     8701 tgtaaattta tttgaactta ttataaataa ttgtattttt tctactattt taggaaatga
     8761 agtgattaat ttagaaattt caaaatatga gtatgaattt ttagaaaaac aatttaaaaa
     8821 tatgtacaat aatattactg aagataaagt tataggctct gaagaaagta atataattga
     8881 attgattgaa gtagatgata gttttaacac tgaagtagat agtaatttgg attataatga
     8941 ttttgaagaa attaacagag aaattagaat aaataaaaaa ataactttac tacttatgga
     9001 agcaattgaa gtattaataa aaagattaag catagaagat gaagatgtaa taagatatat
     9061 taaaaaatat gaaaagatat ttattgaatc tgtgctaagc tcaataaaaa gtaacacttt
     9121 taaaagtatg gtaactgtga gtaaaaataa aaatgaacac ttagaagact atattgttga
     9181 tgatgaaagt aaattggatg atgaatcgtt tagagagata ttcaatgaga tattagatag
     9241 tacatctata attgaaaaaa taagaataat aaaggaaaat atcaatgcta aaaaagactt
     9301 tatcgatatt cttgaatctg aatgtttatt tggagaagag tatttaatgt tgtttgcatc
     9361 attaagtgaa ttagaattgg caatattggg tagtgtagta ttttatgaag atattagaat
     9421 gagagaaatt aatatattag aatttatgtt aaatgagaaa actgaaacta ctttatggaa
     9481 gacagagtac attaaattta tgaagaggca aagtgaagat aagatatact ccatagaaga
     9541 gcatatgaat aaaattaatt aacaaaaaat tgtgaataaa tagaatgtta gtatataata
     9601 taatctgtaa agcatatcaa aaatacatat atttggagga aaattatgaa agagtatatc
     9661 ataaataatg ttgaagaaat aagagaagaa ttaattgatt tatctaagaa aatatgggaa
     9721 aatccagaat tagcctttga agaaaagtat gcaagtagca ttcaaaaaga atacctaaag
     9781 tcaaaaggat ttaaaattga agaagtagaa aatttaccta ctggatttat agcaagtttt
     9841 ggagagggta aacctgtgat tggcatactt ggagaatatg atgctttacc agagctatca
     9901 caatgtgtga gtgcagaaag aaagccattg gtagaaggaa aagctggaca tggatgtggg
     9961 cataacctgt tgggtgtagc aggtgtagga gcagttgttt ctataaaaaa attaatagaa
    10021 gaaaataaat ttaatggaac aataaaatat tttggttgtc ctgctgaaga agaaggtgga
    10081 ggaaagacag ttatgtgtat aaatggctgt tttgatgatg tagattgtgc atttacatgg
    10141 catccttttg atataaatgc accttggaga ggtggaagtc tggcaaactt atctgtaaaa
    10201 tttaaattta aagggataac agcccatgca gctcaagctc ctcataatgg tagaagcgca
    10261 cttgatgctg ttgaaattat gaatgttgga gctaattatt tgagagaaca tgttattgac
    10321 tctataagaa tgcactatgt tataactaat ggtggtggaa gacctaatgt agttcctgga
    10381 tttgctgaaa gttggtactt tattagaggt aaaaaagcaa gggatgcaga acatgttctt
    10441 gatagattaa taaaagtagc tcaaggagca gcaatgatga cagaaactga aatggagtat
    10501 aaggttacag atggaatata tgactatata ccaaaccaat gtttgactga tttagtttat
    10561 gacaatatgg tttttgttgg ttgccctaaa aatactcctg aagaagaaga atttgcaaaa
    10621 aaattgtgtg atactcttac tagagaagaa agattaggtg tagcaacatc attacaaaat
    10681 gataaatcta ttgtagaaag ttatatacac aaggaaatag ttgacggtga caaagataaa
    10741 ggactagctg gttctactga tgttggagat gtaagttatg taataccagt ggctcaattt
    10801 gcaatggcag cttggcctgt tggaattgca agtcatacat ggcagtcatg ttcatcagca
    10861 ggttcaaata taggattttc tgctatgata aattcagcta aagtactggc ttgtagtgct
    10921 tatgatgtat ttatgaatac taaaattatt gatgaagcaa aaattgaatt tgataaatca
    10981 ttagatggac aaaaatttaa accattggta taattttggt aataaaatat ttctgggggg
    11041 aatagctatg agagaaaaag ttgaaaaagt gcttgaggaa aagataaaac cagtattaca
    11101 aagagatggt ggagatgtag agcttataga tgtaaatgaa aatggtgtgg ttttagttag
    11161 actacaaggg gcatgtagtg ggtgtccagg agcgactatg acaatcaaag ctattattga
    11221 aaatgttttg gtgagtgaag ttccaggagt aactcaagta ttaggtgttt aatagttagc
    11281 gtttattaaa tgtatgttat acataagtat ttaataaaga agtaaaaaaa atagtgaagc
    11341 aatatatatg aaaaagtatg ataaagataa taaaacatta tagaaataat gaaatatttc
    11401 atatgtatat gaagaaataa gaaaa
//
LOCUS       DAMJWP010000108        11365 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.113, whole genome shotgun sequence.
ACCESSION   DAMJWP010000108 DAMJWP010000000
VERSION     DAMJWP010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11365
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.113"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(155..982)
                     /locus_tag="PC700_002306"
     CDS             complement(155..982)
                     /locus_tag="PC700_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2581611.1"
                     /translation="MKIKDNKAVFHVFIRIFLIAVLILTGTLLEEKINNVPVENISSK
                     GENTLELLSQSNTLGEIIYKASQEETKGPGATSEKVAYITIDDGPSKYTNALLDILKE
                     NDVKATFFMLNQNMKNHKEEVRRVLEEENSIGFHSVSHDIHKLYKSPEVTVGEFNTCN
                     NTLQEITGQSSKLIRLPYGSKPYMPEGSYNKLMANDYLIWDWNLDTQDWKSSTSQIVS
                     NILYYGRKRDELVVLIHEKEQSLNALNNIIRILKERGYTILPITENITPKNFWQENL"
     gene            complement(1030..2466)
                     /locus_tag="PC700_002307"
     CDS             complement(1030..2466)
                     /locus_tag="PC700_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase"
                     /protein_id="HDF2581612.1"
                     /translation="MNTRNDNSKILEIYKMKLFGELVKAVWNDDIEDIENLPNKILNE
                     DLKDNLNNYFSGKFDKDTMVNLIRVTMGLDVTEDTDKNLKESLHEAVNLDEVKRPIIS
                     VISDACKYCDENNKEDESCKIRSKHINCNEEDVCSACGDCVSRCKLGAISDKIEFIPM
                     IKLLKDIKSPVYAIVAPAFVGQFGKDVSPGKLRTALKKIGFEDMIEVALAADMLTAKE
                     AYDYYEHMKKDEEGCFITSCCCPIWVSLIQNSFPQILENVSPSVSPMIACGRAVKILN
                     PDAKVVFIGPCMAKKKEAMLEDIKDAVDFVLTFKELQEIFDALELNPAIMDESNRVEA
                     SLSGRVYARTGGVSKAVKLSVRRIDKNIKFEEKAFEGTKECMDGLKKVLNKEIEATFI
                     EGMGCVGGCVGGPKRILSVEEGTKNVDEYSESTNMETPFDNLNVLQFLTSLGIKRVES
                     LGEKEEEKVKKIFSRDIKSNKKENKYKK"
     gene            complement(2553..3422)
                     /locus_tag="PC700_002308"
     CDS             complement(2553..3422)
                     /locus_tag="PC700_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDF2581613.1"
                     /translation="MKLQKMGMRTIKTGISVTLCMILGNYLVENMFYSLVACIISVQD
                     TVKGSLKTGINRVEGTILGGIIGFIFAIIKAGDPFLCGLGVMCSIYLCNLFKIKSVVV
                     ACVTFLAIHLGVGNSDPVYYSLHRVLDTSVGVLFGVLVNYYVARPNYLNSVIDEFKKI
                     EELSMLLIEDKILRKENLNVEKFEEEVEKLEEIYSKLIDELDYSLSETNIEKIENALR
                     ICREIHFHMQSIELLENKLYLKEHTYKYLKKTYNVDKLDWNIDEEKSPVFNYHLMKIM
                     IEIELLRKLNKSY"
     gene            complement(3580..4233)
                     /gene="phoU"
                     /locus_tag="PC700_002309"
     CDS             complement(3580..4233)
                     /gene="phoU"
                     /locus_tag="PC700_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDF2581614.1"
                     /translation="MVNNSLDISIDTLKEQTVLMMEKCEEAIEKSVTCMIEKDLEGAR
                     KVVEDDEEINKLREEIRDKSIELIVLKQPMARDLRFIYSLGNISIELERIGDYAANIA
                     REMLKIGGEAYIKELIDIPKMAKECKSMMSQTREALILFDASIAYDVGTKDDIVDGLY
                     EQVQIDCLKIMHENSNTIDQGVKLLLIGRYLERIGDHITNVCEKIIFAIEGKMVEIG"
     gene            complement(4261..5025)
                     /gene="pstB"
                     /locus_tag="PC700_002310"
     CDS             complement(4261..5025)
                     /gene="pstB"
                     /locus_tag="PC700_002310"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDF2581615.1"
                     /translation="MELIDKIKMSVKDLDLFYGDKQALKKINMDIKENKVTALIGPSG
                     CGKSTFIRTLNRMNDLIEDVTIKGNISVDGEDIYTSDDVINLRTKVGMVFQKPNPFPM
                     SIYDNVAYGPRTHGLRDKKQLDKIVEESLKGAAIWDEVKDRLKSSALGLSGGQQQRIC
                     IARAIAMRPEVILMDEPTSALDPISTLKVEELIEDLKKDYTIVIVTHNMQQAARISDE
                     TAFFLNGEVIEFSDTKTMFTTPVDKRTEDYITGRFG"
     gene            complement(5044..5868)
                     /gene="pstA"
                     /locus_tag="PC700_002311"
     CDS             complement(5044..5868)
                     /gene="pstA"
                     /locus_tag="PC700_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDF2581616.1"
                     /translation="MRKFKENLLKSLVYLSALFTIVSLVIIVGFIFIKGIGNMNFNFL
                     FSNYSASGDGGILPMIITTLYTVVVSIVVATPIGILAAIYLQEYAKKGKAVTAIRFAT
                     ESLSGIPSIVYGLFGGIFFVVTLKMGYSIVAGSLTVAIIILPVIIRTTEEALKTVPQA
                     YRESSLALGATKFQTLYKVVLPSAIPGILSGVILSVGRIIGESAAILLTAGTVAKMPG
                     GIFDSARTLTVHSYLLTKESGDIATAASIGIVLIVIVLALNMLARFVAKKLNKANY"
     gene            complement(5870..6787)
                     /gene="pstC"
                     /locus_tag="PC700_002312"
     CDS             complement(5870..6787)
                     /gene="pstC"
                     /locus_tag="PC700_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDF2581617.1"
                     /translation="MIVKSRLVGLEKNDNNGNKTKYVIEKVAKNIFLLSALVAVASLL
                     LIIGFVFYKGLRPFIFEGYSFIDFLTGADWVPSANKFGISSMIVASIFATLGALVIGV
                     PVGILTSVFIAEVAPKRLAKIMSPAVELLAGIPSVLYGVFGLAVIVPTIQSIFNLPKG
                     QSLLAIVIVLAVMMLPTIISVSETAIRAVPKAYKEGSLALGASKIETIFKVVLPAAKS
                     GVLAAVVLGVGRALGETMAVILVAGNSPVMPTAITDSVRPLTTNIALEMGYAFGTHQE
                     MLFATGVVLFTFILILNLVLNKLSNKAVN"
     gene            complement(7169..8404)
                     /locus_tag="PC700_002313"
     CDS             complement(7169..8404)
                     /locus_tag="PC700_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2581618.1"
                     /translation="MRIFYLEIKRVLHSRRTLILLLLALVLSALMAFLPITYEGINYL
                     DNNGKVVELSGLDAIKYKKTIYANNSEISSGVVTPKKLKHALNIWKKTEEKYGKVGSE
                     DFPLDIYSKSILPIRPLLNRLPEAFANSETGFATELSEINTNDLDSFYEQCKTHLNDI
                     MKIERQTSSAQMKAQELYRKVDTPFEIYPGYSKDAFDYIIFYIFILLIICVAITAPTF
                     SGEYQTQSDSILRCTKHGHTRLAFTKILALFSIFIVMFVVCISIHLAISDLAFGTECL
                     KTSFQMLFSVISLVNINLLQAQILIAIGGLISILAMVSLTLFLSAKCKDSLTSMLIAF
                     SICLIPMGIGVSWIGYLLPPGGIGLKISLLYQMTSFNFLHLGGMSFWTPHIILFFSVI
                     EIPIFIFLAIRTYCKHQVS"
     gene            complement(8405..9292)
                     /locus_tag="PC700_002314"
     CDS             complement(8405..9292)
                     /locus_tag="PC700_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2581619.1"
                     /translation="MELKIEHVTKKFKDKIAVEDVCLTLTPGVWGFLGANGAGKTTLM
                     RIITDIMKPTNGKILYNGKNIYIMGAEYRNLLGFLPQEFGFPQDFSVKDYLEYIAALK
                     DVPPREVKGKINNLLEILTLSDVKNKKIIKLSGGMKRRVGIAQAMLNDPKILIMDEPT
                     AGLDPGERVRFRNFISEFSHNRVVLISTHIVSDIEYISTQNAIMKSGKILDVGTTDKL
                     VKKVEGKVWECLISNRDISKYEMNLRIINQRSEKNNQVSIRYLAEKSQIDNSIAVEPR
                     LEDLYLWLFPEAVYSKEGK"
     gene            complement(9437..10552)
                     /locus_tag="PC700_002315"
     CDS             complement(9437..10552)
                     /locus_tag="PC700_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2581620.1"
                     /translation="MKKINNFTYRKLRDKYKFIKREKNTEAYLKYKLYIMLAIYTGIV
                     LVLMFMADYTFSNFSNPLVKWLKLRRDVLFFLVFIIGYITIFNFYWKKPWNYLREIIN
                     ATHTIYQQNDELVELSKPLKEIEQQMNQIKMSVLLSQQAVKEAENKKNNLVMYLAHDI
                     RTPLTSVIGYLSLLKEIPNMDNSQKSKYTEVALDKAQYLEKLINELFEITRYNSNQIK
                     INFEKVDLYYMLLQLKDEFYPLLSEKGNRAILKANESLTIKADSEKLARVFNNILKNA
                     IAYSYPNTEIIISAVHKDSKVDIAFRNYGQTIPEEQLSAIFEKFNRLDEARKSDMGGA
                     GLGLSIAKEIINLHGGDITVQSKDEVTTFTISLPTSI"
     gene            complement(10588..11286)
                     /gene="vanR"
                     /locus_tag="PC700_002316"
     CDS             complement(10588..11286)
                     /gene="vanR"
                     /locus_tag="PC700_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanR-ABDEGLN family response regulator
                     transcription factor"
                     /protein_id="HDF2581621.1"
                     /translation="MSGKILVVDDERDIADLIEVYLTNENYIVYKFYSGLDALNCINL
                     NEFDLAILDVMLPGVNGFSLCQKIREKYTYPIIMLTAKDEETDKITGLTLGADDYVTK
                     PFRPLELVARVKAQIRRYKKYSGSLQSGEELSHIFSFLGLELNTQTYECHLNGKALSL
                     TPTEFSILHVLLKNKGSVVNSENLFHAIWKDEYYNKSNNTITVHIRHLREKLGDTVDE
                     AKYIKTIWGVGYKI"
BASE COUNT         3635 a         2045 c         1266 g         4419 t
ORIGIN      
        1 tcaataattg caagattatt ttattttaat tttaatgctt tctcattaat tttatattat
       61 attatcctta cagtatacct cttattcgta ttttttatat tatatgtttt attttataaa
      121 taatttactt tatcttttac tatatttaaa tttattataa gttttcttgc cagaaatttt
      181 taggtgttat attttctgta attggcaata ttgtatatcc tctctctttt aagattctta
      241 taatattatt taaagcattt aaactttgct ctttctcatg aattaatact actaattcat
      301 ctctttttct tccataatat aatatattac ttactatctg acttgtagaa gacttccaat
      361 cctgtgtatc caaattccag tcccaaatta aataatcatt tgccataagt ttattatatg
      421 aaccttctgg catatatggt ttacttccgt atggtaatct tattaatttt gaactctgac
      481 cagttatttc ttgtagagta ttattgcatg tattaaattc cccaactgtt acttctgggc
      541 ttttatataa cttatgtata tcatgactta cactatgaaa acctattgag ttttcctctt
      601 ccaagactct ccttacttct tccttgtgat ttttcatatt ttgattaagc ataaaaaatg
      661 tcgctttgac atcattttct tttaaaatat caagtagtgc atttgtatat tttgatggac
      721 catcatctat tgttatgtat gctacttttt cagaagtagc ccctggacct tttgtttctt
      781 cttgagatgc tttgtatatg atttctccta aagtattact ttgagaaagc agttccagtg
      841 tattttcacc ttttgaactt atattttcaa ctggaacatt attaattttt tcctccaata
      901 atgttccagt taaaattaaa acagctataa gaaatatcct tataaataca tgaaaaacag
      961 ccttattatc tttaattttc atcccccata tctccctttt ataaaagcat aagtttaaga
     1021 cttaaaaagt tattttttat atttattctc tttcttatta ctttttatat ctctactaaa
     1081 tattttttta accttttctt cttctttttc tcctaaagat tctactcttt tgattcccaa
     1141 agaagttaaa aattgtaata catttaaatt atcaaatgga gtttccatat tcgtactttc
     1201 actatattca tcaacattct tagtaccttc ttccacactt aaaatacgct tcggacctcc
     1261 tacacagcct cctacacacc ccattccttc tataaatgta gcttctattt ctttatttag
     1321 aactttcttt agaccgtcca tgcactcttt tgttccttca aatgcctttt cttcaaattt
     1381 tatatttttg tcaattcttc tgactgataa cttaactgct ttacttacac ctcctgttct
     1441 agcataaact ctacctgaaa gggacgcctc tactctatta ctctcatcca ttattgctgg
     1501 atttaactct aatgcatcaa atatttcttg caactcttta aatgtaagca caaaatcaac
     1561 tgcatctttt atatcttcta acattgcttc tttctttttt gccatacaag gacctatgaa
     1621 tacaacttta gcatctggat ttaaaatttt tacagctctc ccacaagcaa tcataggaga
     1681 aacggatggt gatacattct ccaatatctg aggaaaacta ttttgtatca aactaaccca
     1741 aataggacag caacaactag ttataaagca tccttcttcg tcctttttca tatgctcata
     1801 ataatcatat gcttcttttg cagtaagcat atctgctgcc aaagcaactt ctatcatatc
     1861 ttcaaatcct atctttttta atgcagttct taatttacca gggcttacat ctttcccaaa
     1921 ctgtcctaca aaagctggtg caactattgc atatacaggg ctctttatat cttttaacaa
     1981 ttttatcatt ggtataaatt ctattttatc tgaaattgca cccaacttac atcttgatac
     2041 acaatcacca caagcactac atacatcttc ctcattacaa ttaatatgct tacttcttat
     2101 cttacatgac tcatcttcct tattattttc atcacaatat ttgcaagcat ctgatattac
     2161 agatattatt ggtcttttga cttcatctaa atttacagct tcatgtagtg actcttttaa
     2221 atttttatct gtatcttctg tcacatccaa tcccatagtt accctaatta aatttaccat
     2281 agtatcttta tcaaattttc cactaaaata attatttaaa ttatccttta aatcttcatt
     2341 taaaatttta ttaggtaagt tttcaatatc ctctatatca tcattccaca ctgcttttac
     2401 aagttcgcca aaaagcttca ttttatatat ttctagtatt ttagaattgt catttctagt
     2461 attcataaaa aatagtacct ccataaatta aacttatata tgtttaatat ttacaaagat
     2521 actatttttt atttattatt taactactat tattaatatg atttatttag ctttctcaac
     2581 aactctattt caatcattat tttcattaga tgatagttga atacggggct tttttcttca
     2641 tctatattcc agtctagctt atccacatta tatgttttct taaggtattt atatgtatgc
     2701 tctttcaaat aaagtttatt ttcaagtaac tctatagact gcatatgaaa atgtatttct
     2761 ctacatatcc ttaaggcatt ctcaattttt tctatattag tttcactcaa actataatcc
     2821 aactcatcaa taagttttga atatatttct tctaattttt ctacttcttc ttcaaatttc
     2881 tctacattta aattttcttt tctaagaatt ttatcttcaa taagtaacat tgaaagttct
     2941 tctatctttt taaattcatc tataacacta tttagataat ttggtctagc cacataataa
     3001 ttaacaagta ctccaaacaa aacacctaca gatgtatcta acactctatg taaagaataa
     3061 tacacagggt cactatttcc tacacctaga tgtatagcca gaaaggttac acaggcaact
     3121 actactgact ttattttaaa cagattgcat aaatatattg agcacattac tcctagacca
     3181 cataaaaaag ggtctccagc tttaatgatg gcaaatatga atcctattat accacctaaa
     3241 atagtacctt ccactctatt gattcctgtc tttaaagaac cttttaccgt atcttgaaca
     3301 gaaattatac atgctactag agaataaaac atattctcta ctagatagtt accaagaatc
     3361 atacataaag tgacactaat tcctgttttt atagtcctca tacccatctt ttgaagtttc
     3421 aaagttccac ctcctaaaaa gcaccttata taaaatacgc atcctcatat ttaatatgtt
     3481 cctagtatat cacaattttt taaatttacc cattaatcat atttttttat caattctttc
     3541 ttaatttaga caataaaaaa actgctttaa agcagttttt tatcctatct caaccatttt
     3601 accctcaatt gcaaaaatta tcttctcaca tacatttgtt atatggtctc caattctttc
     3661 aaggtatctt cctataagta gaagttttac tccttggtct atagtatttg aattttcatg
     3721 cattattttt aaacaatcaa tttgtacttg ttcatacaat ccatctacaa tatcatcttt
     3781 agttccaaca tcataagcta tacttgcatc aaataagatt aaagcttctc tagtctgaga
     3841 catcatagac ttacattctt ttgccatctt aggaatatct atcagctctt ttatataagc
     3901 ctctccacct atcttcaaca tttctctagc tatatttgct gcataatctc ctattctttc
     3961 caactctatc gatatattac ctaatgaata aataaatctt aagtcccttg ccattggttg
     4021 ctttaatact attagctcta tactcttatc tcttatttct tctcttaatt tatttatttc
     4081 ttcatcatct tctactactt ttcttgcacc ctctaaatcc ttctctatca tacaagtcac
     4141 agacttttct attgcttctt cacatttttc catcataagt actgtttgtt cttttaaagt
     4201 atcaatactt atatctaaac tattattgac cattgtctaa ctcctttata gttatatatt
     4261 ttaaccaaat ctacctgtta tatagtcttc tgttctttta tctacaggag tggtaaacat
     4321 agtttttgta tcgctaaatt ctataacctc tccatttaag aaaaaagctg tttcatctga
     4381 tatacgagca gcttgttgca tattatgtgt aactatgact attgtgtagt ctttttttaa
     4441 atcctctata agttcctcta cttttaatgt tgaaattggg tctaaagcag atgtaggttc
     4501 atccataaga ataacttctg gtctcatagc aattgctctt gctatacata tacgctgttg
     4561 ttgaccacca gaaagtccta gtgcagatga tttaagcctg tctttaacct catcccaaat
     4621 agctgcacct tttaagcttt cttctacaat tttatctaat tgcttcttat ctcttagtcc
     4681 atgtgtacga ggaccatatg ccacattatc atatatactc ataggaaatg gatttggttt
     4741 ttgaaatacc attcctactt tagtacgaag atttataaca tcatctgagg tatatatgtc
     4801 ctctccatca acagaaatgt ttcctttaat tgttacatct tcgattaagt cattcattct
     4861 atttaacgtt cttataaaag ttgattttcc gcatccagat ggacctatta aagcagtaac
     4921 cttattctct tttatatcca tatttatttt ctttaatgct tgcttatcac cataaaataa
     4981 atccaaatct tttacactca tttttatctt atcgattaat tccattattc acacctctta
     5041 aatttaataa ttagctttat ttaatttctt agcaacaaat cttgctagca tgtttagtgc
     5101 taatacaatt actattaaaa caattcctat actggctgct gttgctatat ctccactctc
     5161 tttagtcagt aagtatgagt gtacagttaa tgttcttgca ctatcaaata ttccacctgg
     5221 catcttggct acagtacctg ctgtaagtaa tattgcagct gattctccaa ttatacgacc
     5281 aactgataag attactccag ataagattcc tggtattgca cttggaagta ctaccttata
     5341 taaagtttga aattttgtag ctcctaatgc caatgatgat tctctataag cttgaggaac
     5401 tgtttttaaa gcttcttctg ttgtacgaat tattactggt aatattatta ttgcaactgt
     5461 aagtgaacct gcaactatgg aatatcccat ttttaatgta actacaaaga aaattcctcc
     5521 aaataatcca tatactatag atggtatccc tgataaactt tcagttgcaa atcttatagc
     5581 tgtaactgct tttccttttt tagcatattc ttgtaaatat atagcagcca atattcctat
     5641 aggagttgct acaactatag aaactactac tgtatataat gttgtaatta tcattggaag
     5701 tataccacca tcacctgatg ctgaataatt gctaaataag aaattaaaat tcatgttgcc
     5761 aattcccttt ataaatataa atcccactat aatgactaat gatactattg taaatagtgc
     5821 tgatagatat actaatgact tcaatagatt ctctttgaac tttctcatct taatttaccg
     5881 ccttatttga aagtttattt aaaactaagt ttaaaattaa tataaatgta aacaatacta
     5941 ctccagttgc aaataacatt tcttgatgtg taccaaatgc gtatcccatt tcaagtgcta
     6001 tattagttgt aagtggtctt acactatctg ttattgcagt tggcataact ggtgagtttc
     6061 ctgcaacaag tattactgcc attgtttcac ctaaagctct tcctactcct aatactactg
     6121 cagctaatac tccagatttc gcagcaggta gtacaacttt gaatattgtt tctatttttg
     6181 atgcaccaag tgctaatgaa ccttctttgt atgcttttgg tactgctctt attgctgttt
     6241 ctgatacaga tattattgtt ggtaacatca ttactgctaa tactattact attgctaaca
     6301 aactttgacc ttttggaaga ttaaatatac tttgtatagt tggaactatc actgcaagac
     6361 caaatacacc atataatact gaaggtatac ctgctaaaag ttcaactgct ggtgacatta
     6421 tttttgctaa tcttttaggt gctacttctg ctataaatac agaggttaat ataccaacag
     6481 gtactcctat cacaagtgct ccaagtgttg caaatattga tgctactatc atagatgata
     6541 ttccaaattt attggcagaa ggaacccaat ctgcaccagt taaaaagtct ataaatgaat
     6601 atccttcaaa tataaatggt cttaagcctt tataaaatac gaatcctatt atcaaaagta
     6661 aacttgcaac tgctactaaa gcactaagta gaaaaatatt ttttgctact ttttcaatta
     6721 cgtattttgt cttattgcca ttattgtcat ttttttctaa acctactaat ctagatttta
     6781 ctatcataat accctcccat taaaatatac ttatcgattt tcgttctgta ttcaaaattt
     6841 aattttttat aattcgtctt catcaaattt cctgtacttt tattctactt tttttaagtt
     6901 aaatctatat taggattaag ttaagcaagt gtaaaattat atttactttt ttaacattaa
     6961 ttttactttt aaaactatat ttgacattta ctttacaata tactgttgtt tctctatatt
     7021 aaaatagcag atatagataa taatacagtt tatgtataat ttattaccta tatctgctat
     7081 taagatttgt taactaaatt taacttttat actacatata taattatcgt atcctctagt
     7141 ttatatcact attatttcta tcaaattgtt atgatacttg atgtttgcaa taggtacgaa
     7201 tagccaaaaa tataaaaata ggtatttcta tcactgagaa aaacagtata atgtgtggtg
     7261 tccaaaaact catacctcct aagtgtaaga aattaaatga tgtcatttga tataataaac
     7321 taatcttaag accaattccc cctggtggta ataggtatcc aatccaacta acacctattc
     7381 ccataggaat taagcaaata gaaaaagcaa tcagcataga agtaagagaa tccttacatt
     7441 ttgcagataa aaataatgtt aaactaacca ttgcaagtat tgaaattaga ccaccaatag
     7501 ctataagaat ctgtgcttgt agtaaattta tatttactag actaatgaca gaaaacaaca
     7561 tttgaaatga ggttttcaaa cattcagtac caaaagctaa atcagaaatt gccaaatgga
     7621 tagaaataca tacaacaaac attactataa atatagaaaa tagagctaat attttagtaa
     7681 acgctagacg agtatgtcca tgtttcgtac aacgaaggat gctatctgat tgtgtctgat
     7741 actcaccaga aaaagtaggt gcagtaattg caacacatat aattaataat ataaaaatgt
     7801 aaaatattat ataatcgaaa gcatctttag aataacctgg gtatatctca aatggagtat
     7861 ctacttttct ataaagttct tgagctttca tttgtgcact ggaagtttgt cgttcaatct
     7921 tcataatatc atttaaatga gttttgcatt gttcataaaa agaatctaaa tcatttgtat
     7981 ttatttctga taattctgtt gcaaatcctg tttctgaatt agcaaatgct tcaggaagtc
     8041 tattcaacaa tgggcgtatt ggtaaaatac ttttagaata aatatctagt gggaaatctt
     8101 cactacctac tttaccatac ttttcttctg tttttttcca tatatttaaa gcatgcttta
     8161 atttttttgg tgttacaact ccactactaa tttcactatt attagcataa attgtttttt
     8221 tatatttaat agcatctaaa ccactcaatt caactacctt gccattatta tctaaataat
     8281 tgataccctc gtaagtaatt ggaagaaaag ccatcaatgc agataaaacc aatgccaaca
     8341 ataataaaat taatgtacga cgtgagtgca acactcgttt aatttccaaa taaaaaatac
     8401 gcatttattt cccctcctta ctataaactg cttctggaaa taaccataaa tataaatctt
     8461 ctagtcgtgg ttcaacagca atagagttat caatttgtga cttttctgcc aagtaacgaa
     8521 tagatacttg attgtttttt tcactacgct gattgattat acgtaaattc atttcatatt
     8581 ttgaaatatc tctatttgat ataagacatt cccatacttt gccctctact ttcttaacca
     8641 gtttatcggt tgttcccaca tcaagaattt ttccactttt cataatagca ttttgagttg
     8701 atatgtactc aatatcagat acaatgtgag tagatattag gacaacacga ttatgagaaa
     8761 attctgaaat aaaattacga aatctcactc gctcaccagg gtcaagccct gctgttggtt
     8821 catccattat aaggatttta gggtcattta acatggcttg tgcaattccc actcgtcgtt
     8881 tcattccacc agataattta ataatctttt tattttttac atcagataat gtaagaattt
     8941 ccaatagatt attaattttt ccttttactt ctcttggtgg tacatctttc aatgctgcta
     9001 tatattctaa ataatctttt acagaaaaat cctgtgggaa accaaattct tgtggtaaaa
     9061 atcccagtaa atttctgtac tcagcaccca ttatataaat attttttcca ttatacagaa
     9121 ttttgccatt ggtaggtttc ataatgtctg taataattct catcaaggta gttttacctg
     9181 ctccgtttgc tcctagaaat ccccacacac ctggtgttaa ggtaaggcaa acatcttcta
     9241 cagcaatttt atccttaaat ttttttgtca catgttcaat ttttaattcc atatttgaaa
     9301 ctcctctctt tatctactta cctgcaaaaa caaaagtgtc ttgcttagta tttattttac
     9361 taaacaaaga cactcataat tttaatatta aatagataat ttcttaagaa aatcctaata
     9421 tttgatttat attttactaa atagatgtag gaagcgatat agtaaatgtt gtgacttcat
     9481 ctttactttg aacagtaata tcaccaccat gcaaatttat aatttcctta gctatagaaa
     9541 gccctaaacc tgcaccaccc atatcagatt ttctagcttc atctaaacga ttgaactttt
     9601 caaatatagc agaaagttgt tcttctggaa ttgtctgtcc ataatttcta aaagcaatat
     9661 ctaccttgct gtctttatga accgctgaga taataatttc tgtattagga tagctgtaag
     9721 caatagcatt tttcaagata ttattaaaca ctcttgcaag tttttcagaa tctgctttta
     9781 ttgttaaact ttcatttgct tttagaatgg ctctatttcc tttttcagac agcaatgggt
     9841 aaaattcatc ctttaattga agtaacatat aatataggtc tactttttcg aaattaattt
     9901 tgatttgatt agaattatat cgtgttattt caaaaagttc attgataagt ttttctaaat
     9961 attgtgcttt gtcaagtgca acttcagtat acttagattt ttgagaatta tccatattag
    10021 gtatttcttt taataagctc aaatacccaa taactgaagt aagtggtgtc cttatatcat
    10081 gtgctagata catcacaagg ttattctttt tattttcagc ctctttcact gcttgttgac
    10141 ttaacaaaac agacatctta atttggttca tttgttgctc tatttccttt aatggcttag
    10201 atagttcaac caattcatca ttttgttgat aaatagtatg tgttgcattg atgatttctc
    10261 ttaaataatt ccatggtttt ttccaataaa aattgaaaat agtgatatat ccaattataa
    10321 aaaccaaaaa aaataataca tctcttctca acttaagcca tttaacaaga ggatttgaaa
    10381 aatttgaaaa cgtataatca gccataaaca ttaaaaccaa tactatacct gtatagatag
    10441 ctaacattat atataattta tactttaaat acgcctctgt atttttttct ctttttataa
    10501 atttatattt atctcttagt ttcctataag taaaattatt aatctttttc ataatactac
    10561 atcccttctt gacaagaaaa ataggtgtca aatcttgtaa ccaacacccc aaattgtttt
    10621 tatatatttt gcttcatcta cagtatcacc taatttttca cgaagatggc gaatatgaac
    10681 tgttatagta ttgttacttt tattgtaata ttcatctttc cagatagcat gaaaaagatt
    10741 ttctgaattt accacagagc ctttattttt caataataca tgtagaatag aaaactctgt
    10801 tggagtaagt gaaagagctt taccatttaa atggcattca tatgtttggg tattcaattc
    10861 cagacctaaa aaagaaaaaa tatgtgaaag ttcttcacca gattgtaaac ttccactgta
    10921 tttcttatat cttctaatct gtgccttcac tctagcaaca agttccaaag gtcggaaagg
    10981 tttagtaaca taatcatctg cacctaaagt taagcctgta atcttgtctg tttcttcatc
    11041 cttagctgtt aacataatga ttggataagt atatttttct cggatttttt ggcataaaga
    11101 aaatccattt actcctggca acataacatc taaaattgca aggtcaaatt catttaaatt
    11161 aatgcagttt agtgcatcaa gaccagaata aaatttataa acaatatagt tttcatttgt
    11221 aaggtagact tctattaaat cagctatatc acgttcgtca tctacaacta aaatttttcc
    11281 actcatagac tttaaacatc ctttctatat atatttatat atattatact aaatttaatt
    11341 tttttatatg attttttaca ttaga
//
LOCUS       DAMJWP010000109        11299 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.114, whole genome shotgun sequence.
ACCESSION   DAMJWP010000109 DAMJWP010000000
VERSION     DAMJWP010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11299
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.114"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            198..899
                     /locus_tag="PC700_002317"
     CDS             198..899
                     /locus_tag="PC700_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HDF2581622.1"
                     /translation="MKFQEMINSPGLWIVSSFLVIISVVQAIVFMKSALKEASNLGIE
                     RKGIKAAIRSASVTAIGPSLSPVITLLSLVAVIGAPTTWMRLCDVGAARTELGVISLT
                     SNLSGVEVGSAAFGAEAFSYALWGMALNNLGWLLVVFILGHRMRGIVEKMNVKYNPTW
                     VKKLLAGATVGLFAYLLSNQIKTFEIPKLTPAVISAIVMLVLTTLFKKNQRLQELSLG
                     IAMLIGMFGTQIVLS"
     gene            925..1581
                     /locus_tag="PC700_002318"
     CDS             925..1581
                     /locus_tag="PC700_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581623.1"
                     /translation="MYDKKMIKIGRISIGLAIIANFIPAIYVGLRFGQMPMLSVIFQI
                     WGLVATTYGVSWIVQPIAYYPTLGAPGSYIGWLAGSVGDIRMPAASMAQKIAGVEAGT
                     HEGEVVGTIGTCCSIFVSASMITIFTIVGYKVIPLLPEFITDSFNYILPALFGAIYVD
                     IARKDIRAGGCTIAVALAIMYFGEKIGIPGGLLTLFIVLGGILITRVFFVIDSNKTKE
                     "
     gene            1788..3428
                     /locus_tag="PC700_002319"
     CDS             1788..3428
                     /locus_tag="PC700_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HDF2581624.1"
                     /translation="MFDVKIANGIIIDGTGNSRYKGDVGIVGDKIMAIGDLSQKEAKE
                     TIDATGKFVSPGFIDFHTHSDLSLVYDKYTRSRIHTGVTTDVIANCGIGVAPIREEKK
                     QELIDYLGTRIIGTIPIKLNLHWNTMQEYFDYLTENSPAVNVVAYVAQGPVRINEMGF
                     SKEPATLEQLKNMKLEVRKAMEAGCVALSSGLVYLPGAYTKKEEMAELCKELIPYNGY
                     YISHIRDEGDEEMEALDEAIYIAKTAGVPLHISHLKVMGHKNFGTIDKVFKKLDEAEA
                     DGLEVTFDCYPYTAGMTSLGALLPPWAFEGGVENMVKRLEVQENRDRIIKELEEGIPG
                     WQCFYQLAGGWDGVVLASVMTEANKYVEGKTLMEVAKINGENPFDTFFRLLIEEKSKI
                     QVVVHTQGQEDTDKVVCHPKSCIGSDSMDLSTEGLLSLGKPHPRAFGTFGRIFSYYVR
                     EKGMLTFEEAVKKITYLSAKRLGIYKERGLLKENYFADIVVFDPDTIEDKATYSNPKQ
                     YTVGVEYVLVNGKIALAGGKQTDVCAGRVIKNPLSIAK"
     gene            3787..5109
                     /locus_tag="PC700_002320"
     CDS             3787..5109
                     /locus_tag="PC700_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581625.1"
                     /translation="MKILYVGPGMVIDKILAVSKKNFPNIEADFLRYDRYIEVPDLLE
                     KYRGRVDAVLFTGKSPFKFFEKRQKDFVLSDYIPRHETTLYRVLLEITYLLKFDISRL
                     SIDTYDKAMLKRLYKEIGVEFKDEHMFFAEQKYLEEDYIKFVLEFHKYNYQNNDICCC
                     ITGLEEAYHAMKKENIPVVLALPSEDVMVQSIKNIQMRYIAKKNNENQIVVLAIKLNM
                     PSEYSLMKEDEYAYLSQRIKILDKLYHFNSRIDGVLVEQSRSEFMIFTTKKILELETK
                     KYRDIYLLDMIREVSVINTYIGIGYGKTANEAKFNAYESIKMAQSQKNDAAYVVFENG
                     EIMGPLKPNPAVKEKNSFDERFYQIATETGLSANTVYKVFNKIINSGKTEFTSKELAI
                     ICGVSIRTMDRIILRLCDSGYCEVVSEQLMSKYGRPSRILRFKPFLLF"
     gene            5306..6313
                     /locus_tag="PC700_002321"
     CDS             5306..6313
                     /locus_tag="PC700_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HDF2581626.1"
                     /translation="MGKYQDSKKVVRNYFEALENATGDEVENVLKQHMKSDYNWKGVY
                     PFREQEGTENVADVFWKPLKKSLSNMQRRQDIFIAGTNEIDNSEWVMSMGHFMGLFDK
                     DWLGIKHTRKMISLRYAEFNCVQDGKIAKTGLFVDIIGFMIQAGVNPLPPQTGSYFVY
                     PGPIDHNGLLFEDADEVEGVKTLALVNKMVDDLSELNESGTMGCPPEILKKSWAKDMI
                     WYGPGGIGASYTIPRYQEQHQLPFRNNLKGKTFNGHVCRFAEGNFACFFGWPNLTNVP
                     CGGFLGLPGGEVKADMQVVDVYYRKGDKLQENWVLIDIPYWLKQQGLDILERTKSIFN
                     A"
     gene            6351..7748
                     /locus_tag="PC700_002322"
     CDS             6351..7748
                     /locus_tag="PC700_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HDF2581627.1"
                     /translation="MASTNKNNVTSKEEEKEVSTSGLESCTVKENKAKMNQTNFSNIN
                     ENQTNENKTNTYKGNIDKINTNKSDINKVNIDTVDTDKLNTNTLTEKNKKVIFKGEDN
                     MSSTKIYYGDSLEVTPVGVMDYNDFSKQTKREQEIPGFDSKYRDFVDYIMKITHNIWE
                     EKGIGVIYDTYHNNVTMHCGSSNLVGIKDVISNTLQTLHAFPDRRLIGQNVIWSNFGA
                     DGFLSSHRVLSTATNLGDSNFGPATGRKINFRTVIDCATTNNRIHEEWLVRDNLWIVT
                     QLGLNPQEVAKSMAKASESKVLSLQSTYGICESMDGQFMPTKYQAKDDSVGEMMLEMT
                     SRIYNYKYINEVKKYYHDNAVVHFICDKDLNGYDEIQGMIVSLLASIPNGSYEVERVT
                     CNQREKNEGYDVSIRWRLRGINEGIGFFGQPSGKHIEVMGINHYHILQGKVKEEWITF
                     DGMDVLKQMYMGVEE"
     gene            7893..9158
                     /locus_tag="PC700_002323"
     CDS             7893..9158
                     /locus_tag="PC700_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2581628.1"
                     /translation="MKTTVEIENDIILGKDYSNLKRGIAFFSIALIYFFYCYNFMVGT
                     FIKPTMIYALADGGFGFSLKQTEEIFAVMSFGTIPGTFIFGVISTKIGKKRTLISVAL
                     LIGLTTFIPMLSPTDIMLWKIARLCTGVVLGGVFGTAMPLVADMFPSKYRGKLAAILT
                     ALFSLAMIFGGKVYGVLGDANWQILMYTAIIPPIVGAILAIFFVPDDLEYTKELVKKG
                     KETGEKISYISMYKGKYLWIGLGTILLSGANFTAYSAFSNNATTYLVTGIGMSAAVAG
                     SIYSLQGIGQLVGYLFWGSIADKFGRKIPFIGMALAAVFVFIYTKLGGDNTTTFYMIS
                     VLLGVAVGYSGAWGAYYTELFPSKYRSLSSGMSFNGGRIISTFAIPAIAGTASGTLGM
                     MTIFYISIAVFVAGAIVWLFLPETLNNKK"
     gene            9200..10239
                     /locus_tag="PC700_002324"
                     /pseudo
     CDS             9200..10239
                     /locus_tag="PC700_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
     gene            10274..11296
                     /locus_tag="PC700_002325"
     CDS             10274..11296
                     /locus_tag="PC700_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF2581629.1"
                     /translation="MNQKKVHYNKSATSKDVARLAGVSQSSVSRAFGSANGKGVKPEV
                     RERIFRVANEIGYVPNLVARGMISGKTNVIGLIVGDSLGPFYNRIINLFVEKIQEIGK
                     QCLVFKVPRQQSIDSIISKVIQFQVEAVIITASAMNKVMAETCEENNIPVILFNRFIP
                     GIKISTVYVDPIEGGGMVAEYLLKKGHKNIGYIQFTRETSEEMEKKIGFYSKLRQSGI
                     HNLKEESANYDYDSGYEAGKRMLALSNPPTAIFCTSDLIAIGVMDAARFEYKLRVPED
                     LSVIGYDDIQMASWKSYNLTTIRQPLDLLIEKTINILKSLLNEENPESVVEMLKPELI
                     ERGSTL"
BASE COUNT         4062 a         1292 c         2275 g         3670 t
ORIGIN      
        1 attttctgat atttactaat tatcaataaa gtatttaagt attaatgttt ttagtgagac
       61 tttaagacag ataatctcct attaaagata ctatatctta gataaaataa attaaaacaa
      121 tataaaaaca taacaagatt acagaaagaa agctcataat aaaacactaa aaaatttatt
      181 ttaaggagga ataaagtatg aagtttcaag aaatgattaa tagcccaggt ctttggattg
      241 tttctagttt tttagtaatt atttcagttg ttcaagcaat cgtatttatg aaatcagcac
      301 ttaaagaggc ttcaaatctt ggtattgaaa gaaaaggaat taaagcagca attcgctcag
      361 ctagtgttac agcaattgga ccctctctat ctcctgttat tacattatta tcactggtag
      421 ctgtgattgg tgctcctacg acttggatga ggctttgtga tgtaggagct gccagaacag
      481 aacttggagt aatatctctt acatcaaatt tatctggagt agaagtaggt agtgctgcat
      541 ttggagcaga agctttttcg tatgcactat ggggaatggc tttaaataac ttaggttggt
      601 tgcttgttgt atttattttg ggtcatagaa tgcgtggaat tgttgaaaaa atgaatgtga
      661 aatataatcc tacttgggtt aaaaaactac ttgcaggggc tacagttgga ttatttgctt
      721 accttctttc aaatcaaata aaaacattcg aaataccaaa acttacacca gcagttattt
      781 cagctatagt tatgctagta ttgacgactt tatttaaaaa gaatcaacga ttacaagaat
      841 tatccttagg tattgccatg ttgattggta tgtttggcac acagattgta ctatcatgat
      901 taaaacagat aagaggtgat aattatgtat gataaaaaga tgataaaaat agggagaatt
      961 tctataggtc ttgcaattat tgcaaatttt attcctgcaa tctatgtagg tcttcgcttt
     1021 ggtcagatgc caatgctttc tgttatattc caaatttggg gattagttgc tacaacttat
     1081 ggagtgagtt ggattgttca acctatagct tattatccaa cactaggagc tccaggttct
     1141 tatataggtt ggcttgcagg ttctgttgga gatatacgta tgcctgcagc atcaatggca
     1201 cagaaaattg caggtgtaga agcaggaact catgagggtg aagttgtagg aacaattggt
     1261 acatgttgtt caatctttgt atcagcttct atgattacta tttttaccat tgtaggctat
     1321 aaagtaattc ctttgttacc tgaatttatt actgattcat ttaattatat acttccagca
     1381 ttgtttgggg caatttacgt tgatattgcg cgtaaagata ttcgagctgg tggatgtacg
     1441 attgctgtag cattagctat tatgtacttt ggtgaaaaaa ttggtatacc aggtggattg
     1501 ttgacattgt ttattgtgtt aggtgggata cttattacaa gggttttctt tgtaattgat
     1561 agcaataaaa cgaaagaata ggattgttta ctaggaagaa agactagaat tatttgctga
     1621 gaaaaagaat aggattgttt actaggagga aagactagaa ttatttgctg agaaaaagaa
     1681 taggattgtt tactaggagg aaagactaga attatttgtt gagaaaaaag aataggattg
     1741 tttattaaga aatatgaata taaataagtc ttgaaaggag aagaagaatg tttgatgtta
     1801 aaattgccaa tggaataatc attgatggta caggaaattc aagatataaa ggtgatgtag
     1861 ggattgtagg agacaaaatc atggctattg gagatctaag tcaaaaagag gctaaagaga
     1921 ctatagacgc aactggaaaa tttgtatcac ctggttttat agatttccac actcattctg
     1981 atttatctct tgtgtatgac aaatacacaa gaagccgtat ccatacaggt gtaacaacag
     2041 acgttattgc aaactgtggt ataggagttg ctccgataag agaagagaag aagcaggagt
     2101 taattgacta tcttggaacg aggataattg gtacaattcc aataaaattg aaccttcact
     2161 ggaatacaat gcaggaatac tttgattact taactgaaaa ttctccagca gtaaatgttg
     2221 tagcttatgt agcacaagga cctgtacgta tcaatgaaat gggattttca aaggagccag
     2281 ctactttaga gcaacttaaa aatatgaagt tggaagttag aaaggctatg gaagctggat
     2341 gtgtagctct ttcgtcagga ttagtttatt taccaggagc atatacaaag aaagaagaaa
     2401 tggctgaatt atgtaaggaa ttgataccat acaatggata ctatatatct catattagag
     2461 atgagggtga tgaagaaatg gaagctcttg atgaagcaat ttatatagct aagacggcag
     2521 gagttcctct acatatttct catttaaaag tcatgggtca taagaatttt ggaactattg
     2581 ataaggtatt taaaaagcta gatgaagctg aggcagatgg cttggaagtt acttttgatt
     2641 gttacccata tacagctgga atgacatctt tgggtgcact tttacctcct tgggcttttg
     2701 aaggtggtgt agaaaacatg gtaaaacgat tagaagtgca agaaaatagg gacagaatta
     2761 taaaagagct agaggaaggg attcctggat ggcaatgttt ctaccagtta gcaggtggtt
     2821 gggacggtgt tgtactagcc tctgtaatga ctgaagcaaa taaatatgta gagggtaaaa
     2881 ctttaatgga ggtagctaaa atcaatggtg aaaatccatt tgatacgttc tttaggcttt
     2941 tgattgaaga aaaaagtaaa attcaagtag ttgttcatac acaaggtcaa gaggatacag
     3001 ataaggtcgt ttgccatcca aagtcatgta taggttctga tagtatggac ctttctacag
     3061 aaggcttact ttctcttgga aagcctcatc cccgtgcatt tggtactttt ggacgaattt
     3121 tctcttacta tgttagagaa aaaggtatgt tgacttttga ggaagcagtt aaaaagataa
     3181 cttatctttc agcaaaacgc ttaggaattt ataaagaaag gggtctactt aaggaaaatt
     3241 attttgctga cattgtggta tttgacccag atacaataga agataaagcg acttatagta
     3301 atcctaaaca gtatacagtg ggagtggaat atgtattggt aaatggtaaa attgcattgg
     3361 caggaggaaa gcaaactgat gtatgtgctg gacgtgtaat taaaaatcct cttagcatag
     3421 caaaatagaa tacattaaga acttttataa aaatagtctt tctttgaata ttaaggaagg
     3481 ctatttttgt taaattaata aagattactc tacttgtgtt ttctagagaa tttaacttta
     3541 agtagaaaaa acaatatatt aattgtaaaa tgtaattaat attaatggat aagatttaaa
     3601 tacaaaaact taaatacaaa aatgttttaa cagatagtca taattaaaaa acttaaagta
     3661 actttttata taagatataa ttgattatgc aaaattttat gaagcagcaa ccttgttact
     3721 ttaagtcaag ggaggttcag aaagtgtctt aagagataat agtatcttta ggaaaggaga
     3781 aacgaaatga aaatacttta tgtaggacca ggcatggtaa tcgacaaaat actagcagta
     3841 agtaaaaaga attttcccaa tattgaagca gattttctta gatatgatag atatattgaa
     3901 gtacctgatt tattggaaaa atatagaggt agagtagatg cagttctttt tacaggtaaa
     3961 tcgccattta aattttttga aaaaagacaa aaagattttg tgttgtctga ttatattcct
     4021 agacatgaaa caacattata cagagtattg cttgaaatta catatttgct taaattcgat
     4081 attagtagac ttagtataga tacttatgat aaagctatgc tgaagagatt gtataaagaa
     4141 attggagttg aatttaagga tgaacatatg ttttttgcag agcaaaagta tttagaagag
     4201 gactacataa agtttgtgct tgaatttcat aaatataatt atcaaaataa tgacatatgt
     4261 tgttgtatta caggtcttga agaagcatat catgcaatga aaaaagagaa tattccagta
     4321 gttttagcct tgccttctga ggatgtaatg gtacaaagta taaaaaacat tcagatgcgt
     4381 tatattgcaa agaaaaacaa tgagaatcag attgttgttc ttgctatcaa acttaatatg
     4441 ccttcagaat attcactgat gaaggaagat gagtacgctt atctttcaca aaggattaag
     4501 attcttgata aactatacca tttcaatagc cgtatagatg gtgttttggt agagcagagt
     4561 agaagcgaat ttatgatttt tacaactaaa aaaatacttg agttagaaac aaagaagtac
     4621 agagatattt atttgttgga tatgattcgt gaagtttcag ttataaacac ttatatagga
     4681 attggttatg gaaaaacagc aaatgaagct aaatttaacg cttatgagag tattaagatg
     4741 gctcaaagtc aaaaaaatga tgcagcatat gtagtttttg aaaatggaga aattatggga
     4801 ccactaaagc caaatcctgc tgtcaaagag aaaaatagct ttgatgaacg attttatcaa
     4861 attgctacag agacagggct aagtgctaat actgtatata aagtgttcaa taaaataata
     4921 aatagtggaa aaactgaatt tacatcaaag gaattagcta ttatatgtgg tgtaagtatt
     4981 agaactatgg acaggattat acttagattg tgtgattctg gttattgtga ggtagtgagt
     5041 gaacagctta tgagcaagta tggtagaccc agtagaatat tacgttttaa accttttctg
     5101 ttgttttaaa tagatataag ttaaaaatta aatttttatg ataacatttt ctttacaatt
     5161 ttttagatat atgttaatat ttagaaaaat gcatatgtat tcataaaata aactcaaaat
     5221 agctaaaaaa tcattaaaaa ggaaatcgta tgcataaaat gagaaagtta atttataact
     5281 taaataaatc tgggaggaga taattatggg aaaatatcag gattcaaaaa aagttgtaag
     5341 aaattatttt gaagcattag aaaatgctac tggggatgag gttgaaaatg tcttaaagca
     5401 acatatgaaa tcagattata actggaaagg tgtgtatcca tttcgtgaac aagaaggcac
     5461 tgaaaatgta gcagatgtat tttggaagcc tttaaaaaaa tcattgagta atatgcaaag
     5521 acgacaagat attttcattg caggaacaaa tgagattgac aatagtgaat gggtaatgag
     5581 tatggggcat tttatgggac tttttgataa ggattggctt ggcattaaac atactcgtaa
     5641 aatgattagt ttaagatatg cagaatttaa ctgtgttcaa gatggtaaaa ttgctaaaac
     5701 tggattattt gtagatataa ttgggtttat gattcaagca ggagttaatc cattgccacc
     5761 acaaacaggt tcatattttg tatatccagg gccaattgac cataatggat tactttttga
     5821 agatgcagat gaagttgaag gagtaaaaac tttagcatta gtaaataaaa tggtagatga
     5881 tttatctgaa ttaaatgaaa gtggtactat gggatgccca ccagaaatac ttaaaaaatc
     5941 ttgggctaaa gatatgattt ggtatggtcc aggtggaatt ggtgcttctt atactatacc
     6001 tagatatcaa gagcaacacc agctaccatt tagaaataat ttaaaaggaa agactttcaa
     6061 tgggcatgta tgtagatttg cagaaggtaa ttttgcttgt ttctttggat ggcctaatct
     6121 gactaatgta ccatgtggag gttttttagg attaccaggt ggagaggtta aagcagatat
     6181 gcaagttgtt gatgtttatt atcgtaaggg cgataaactt caagaaaact gggtgttaat
     6241 tgatattcct tattggttga aacaacaagg gcttgatatt cttgagagaa ctaaaagtat
     6301 ttttaatgct tagttatgca tacgtattct aaaacaaagg gggatataaa gtggcatcta
     6361 ccaacaaaaa taatgtaaca agtaaagagg aagaaaaaga agtttctaca agtgggcttg
     6421 aatcatgtac ggttaaagaa aacaaagcta aaatgaatca aactaatttt agtaatataa
     6481 atgaaaatca aacaaatgag aataaaacga acacatataa aggaaatata gataaaataa
     6541 acacaaataa atccgacata aataaagtta acatagatac agtggataca gataaattaa
     6601 atacaaatac tcttactgaa aaaaataaaa aagttatatt taagggggaa gataatatgt
     6661 catctacaaa aatttactat ggagattcat tagaagtaac gcctgttgga gtaatggatt
     6721 acaatgattt ttcaaaacag acaaaaaggg aacaggaaat acctggattt gattctaagt
     6781 atagagattt tgtagattat attatgaaga ttacacataa tatctgggaa gaaaaaggta
     6841 taggtgttat ttatgataca tatcacaata atgttacaat gcattgtggc tcttcaaatt
     6901 tagttggtat taaagatgtt atttccaata ctcttcaaac attacatgcg tttcctgata
     6961 gaagattaat tggtcaaaat gtaatatggt ctaactttgg tgctgatggt tttttatctt
     7021 ctcaccgtgt tttgtctaca gcaactaact taggtgatag taattttgga ccagcaacag
     7081 gtagaaaaat aaactttaga actgtaattg attgtgccac aactaacaat agaatccacg
     7141 aagaatggtt ggttagagat aatttatgga ttgttactca attaggatta aaccctcagg
     7201 aagtagcaaa gagtatggca aaggcaagtg aatctaaagt gcttagctta caatcaactt
     7261 atggtatctg tgaatctatg gatggtcaat ttatgccaac taagtatcaa gcaaaagatg
     7321 attctgttgg cgaaatgatg cttgaaatga caagccgtat ttataattat aaatatataa
     7381 atgaagttaa aaaatactat catgataatg cagtagtaca ttttatatgt gataaagatt
     7441 taaatggtta tgatgagatt caaggaatga ttgtaagttt acttgcatca attccaaatg
     7501 gaagttatga agtggaaaga gtaacttgta atcaaagaga aaaaaatgaa ggttatgatg
     7561 tatctataag atggagatta cgtggaataa atgaaggtat aggatttttt ggacagcctt
     7621 ctggaaagca tatagaagta atgggaatca atcattatca tatacttcaa ggtaaagtaa
     7681 aggaagagtg gataactttt gatggtatgg atgtattaaa acaaatgtat atgggagtag
     7741 aagaataaag ctgtttagag tataaataat tttgaaatgt atatagggaa gtattgcttt
     7801 ataaaattta agtggggaat gtatatgaaa aatagatgtt taaactttta gcaaaaatta
     7861 tgactaaatt taaaaggggg aattataaaa atatgaaaac aacggttgaa atagaaaatg
     7921 atatcatatt aggaaaagac tattcaaacc taaaaagagg aattgcattt ttttcaattg
     7981 ctttaatata ctttttttat tgctacaatt ttatggtggg tacttttatt aaaccaacaa
     8041 tgatttatgc tttagcagat gggggatttg gcttttcatt aaaacaaaca gaagaaatat
     8101 ttgcagttat gtcttttggt acaattccag gtacatttat ttttggggtg atttctacaa
     8161 aaattgggaa gaagaggact ttaattagcg ttgctttact gattggattg acaacattta
     8221 taccaatgtt atctcctaca gatattatgt tatggaaaat agcacgtttg tgtacaggag
     8281 tagtgttggg aggcgtattt ggaacagcta tgcctttagt tgcagatatg tttccaagta
     8341 aatatcgtgg taagttagct gctatattaa ctgctttatt ttcattagca atgatttttg
     8401 gtgggaaagt atatggcgtt ttaggtgatg caaattggca aatattgatg tatactgcga
     8461 ttataccacc tatagttgga gctatcttag caatcttctt tgtgccagat gatttggagt
     8521 atacaaagga attagttaaa aagggaaaag aaactggtga aaagattagc tatataagta
     8581 tgtataaggg aaaatactta tggattggat tgggaacgat tcttttatca ggtgcaaatt
     8641 ttacagcata ttcagcattt tcaaataatg caactacata cctagtgaca ggaataggaa
     8701 tgtcagcagc agtggctggg tctatatata gtttacaagg aattggtcaa ttagtaggat
     8761 atttattctg gggttcaatt gcagataaat ttggaagaaa aatacctttt ataggtatgg
     8821 cattagcagc agtatttgtt tttatatata caaaattagg tggagataat acaacaacat
     8881 tctatatgat atcagtatta ctaggtgttg ctgttggata ttctggagca tggggagcat
     8941 actacacaga gttgtttcca agtaaatatc gttcgttgtc atctggtatg tcatttaatg
     9001 gaggaaggat tatatctacg tttgcaatac ctgctatagc tggtacagct tctggtactt
     9061 taggtatgat gactatcttt tacatatcaa tcgcagtctt tgtagcaggt gctattgtat
     9121 ggttattctt accagagacg ttaaataata aaaaataaaa tactaggaat ttattttgtt
     9181 acttagaaag gggtggctta tgatttcatc aattgatgta tgtattataa taggatattt
     9241 gctattaatg ttagttattg gttattattc aggaaaagat aataaaaatc aagaagatta
     9301 tttcttggca ggtaggtcaa tgccatggat tccaattgct ttatcagttg cagctactat
     9361 gattagtgca aatggcttta taggtggtcc tggatgggca tatgtagatg gaatgtatcc
     9421 agtaatgtaa aatgtgcaaa aaattctttt gtagttagtg catttgttat gaatttagtt
     9481 tactacttta tgctattggt aggagtaatt ttatttgttt tttatggggg aaggacattt
     9541 gaaacttcaa atgaaataat gattactttt attttaaatg aacttccagt aggagccatt
     9601 ggaattatta ttgcaggtgt gtttgcagca gcaatgtcta gtatcgactc tatattaaac
     9661 agtatgacaa ctgtttttac gaaggatatt tatgagtttt atttcaaaaa agacaacaaa
     9721 gaatcatcat taaaagttac aatgattata actgtagtta taggagttgt tatgacaggt
     9781 tttattatta tgggatttgg aggaagcatc aaatcaattc ttgaccttgt tggtaactat
     9841 atctcatact ttgctggacc agctacaggt gcatttgtac tagcaatgtt tacatataaa
     9901 gctaatgata aaggagtatc tattggtttt attgtagggt tagttttagg attttttatt
     9961 tctaataaat ataatgtgag ttggttgtgg aatccagcaa taggtgctac aataacattg
    10021 atatttggat atatttttag tgtcatttta aagaaaaatc ataaagtaga tgatattaaa
    10081 aaatacactg catttggtat gagaaatcac cttatttctc aaggaatgga taaagtagat
    10141 ggagtatcta tagtgccatt ttctttaggt aaacaagaac taattgtatt agcttttttt
    10201 attatccaat atattatatt atttttgata caattttaag aagaaatact agatatttaa
    10261 ggagaaatac taaatgaatc aaaagaaagt tcattataat aaatcggcta catcaaaaga
    10321 tgtcgcaaga ttggcaggag tatctcaatc atctgtttca agagcatttg gtagtgcaaa
    10381 tggaaagggc gtaaaacctg aagtaagaga aagaattttt cgtgtagcaa acgaaatagg
    10441 gtatgttcct aatttggtag cacggggaat gattagtgga aaaactaatg ttataggttt
    10501 gatagttggt gatagtctag gtccatttta caatagaatt atcaatttat ttgtagaaaa
    10561 aattcaagag ataggtaaac agtgtctcgt ttttaaagtt cctagacaac aaagtataga
    10621 tagtataatt tcaaaagtaa ttcagtttca agtagaagct gtgattatta cagcttcggc
    10681 catgaataag gtgatggctg agacttgtga agaaaacaat attcctgtga ttctttttaa
    10741 tcgttttata cctggtataa aaataagtac agtctatgta gacccaattg aaggtggggg
    10801 catggttgct gaatatctgc ttaagaaagg tcataagaat ataggatata ttcaatttac
    10861 gagagaaaca agtgaagaaa tggaaaagaa aattggattt tattctaagt taagacaaag
    10921 tgggattcat aaccttaaag aagaatctgc aaactatgat tatgattctg gatatgaagc
    10981 aggtaagaga atgttagcat tatcaaatcc tccaacagca attttttgca caagtgactt
    11041 gatagctatt ggtgtaatgg atgctgcaag gtttgagtac aagttgaggg tgccagagga
    11101 tttatcagtg ataggatatg atgatattca aatggcttca tggaagagtt ataatttaac
    11161 gacgattaga caaccactag atttgttgat tgaaaaaaca attaacatac ttaagtcctt
    11221 attaaatgaa gaaaatccag agtcagtcgt tgaaatgtta aaacctgaat tgattgaaag
    11281 aggttctaca ctttaagtg
//
LOCUS       DAMJWP010000110        11290 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.115, whole genome shotgun sequence.
ACCESSION   DAMJWP010000110 DAMJWP010000000
VERSION     DAMJWP010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11290
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.115"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            413..1273
                     /locus_tag="PC700_002326"
     CDS             413..1273
                     /locus_tag="PC700_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2581630.1"
                     /translation="MKIIQILNNNVALVKRGKNEIIVFVKGIGFKKRVGQVINENEIE
                     RTYILDSYDMLEHFSYLLQNSDSNNIVLINEIIEYGEKALNIKSSDYLSLTLLDHLEF
                     LLKRCEKNQFIKSPLVWNVKRFYPKHFEIGMHALKLIGDSKGINLPDDEAVSIALHFV
                     NMEVNKESHDSTIVELRTLADIVSIIKYHFNVELDETSTNYMRFTTHLQYFIQRIMSD
                     EASIDEDNSIDLYKQVSQLYPKSFGSVQKIRKYIKSQFNVDISISEETYLMLHINRVT
                     QRLEAKSDEI"
     gene            1263..3134
                     /locus_tag="PC700_002327"
     CDS             1263..3134
                     /locus_tag="PC700_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2581631.1"
                     /translation="MKYKDLNENIIKLIGGKENIKSVAHCVTRLRLTLNDRSIAQTEE
                     IKKLKGVIDVVSNDVAYQIIIGTHVVDVYNEFMSMIGLSSSESSIKSERKKGIRGIKG
                     IFNSIFVVVSETMTSVIEVLLAAGILASILALLNMSGILQSDSPTYQILDTLRSATFH
                     FLPVLIAIASAKRLNVNPYIAVVLAVTLLSSSIDGVEGLSLFGINLQATTYANSFIPI
                     LLGVWAMGIIIEGLKKILPKALHYFLVPVLSLIITLSVTLTVFGPIGMWIGDGLNYAC
                     MFLIDTLGNWSVVALYAALQPLLIVFGAANFTMPISLNSVTTLGYDPIFMGAATISDI
                     AVCGAMFGYFLKTRNKEQKQLFGTVSFSALMGVTEPSVFGVFMKYRRPFVAVMIGGGI
                     GGLIAGLAQVKTYGMVWGLAALPTYLVGGEASNFTFMIISVVVSFVAATAASYLLGIP
                     SDEKEEVKGDNEKELELIINENNSRTISIGNVAKGQVIALGEVNDKAFSSGALGKGVG
                     VIPSESKSTVISPVDGTITIVFPTKHAYGIVTDEGLEILIHIGIDTVNLDGKFFESLV
                     TQNQRVKKGDALAVFESDKIIEEGFDSTIITVVTNTSDYLDVISSNDESEELLTVIV"
     gene            3154..4608
                     /locus_tag="PC700_002328"
     CDS             3154..4608
                     /locus_tag="PC700_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDF2581632.1"
                     /translation="MNIKDTFFWGGSIAAHQCEGSWDSDNKGPAIMDFVTKGSYETPR
                     VITDKIEEKLDYPSHNGIDFYNRYKEDIALFKEMGFSALRISIDWSRIFPNGDDENPN
                     ELGIKYYEGLIDTLIENNIEPIVTLYHFELPMNLVHKYGSWNNRKLMDLYLKYSETVI
                     RRFDDKVKYWITFNEMNHIDPQTEHSDIFTYMIAGIKYSEIENKVQTLANVGYNMTLA
                     GVKVAKLAREINPNNKVGCVFGLNPIYPYNCNPSNVLNAFLDNDRDYYQIDAMCNGKF
                     PQYKLKEYEKNNITIEVTDEDKKAFKEGKLDFIGLNYYFSSVSTPKENLSGDKSLFGG
                     IQNPYLEQSKWGWAIDPIGLRFTLNYIYRKYQLPILITENGLGAYDKIEEDGTINDDY
                     RIEYLSKHLSQMKSAIEEDNVECFGYLMWGPIDLVSATTGQMSKRYGFIYVDLDDEGK
                     GSFKRYKKKSFDWYKEVIKSNGEVLDFSTDIVVG"
     gene            5632..6840
                     /locus_tag="PC700_002329"
     CDS             5632..6840
                     /locus_tag="PC700_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581633.1"
                     /translation="MENFWNPLTCFTVCVMIYAFSEFLSKKTKGAISTLLFVCIIFLL
                     GFWTNVLPKDITGASGILAAMSNFGIALLITNLGTLINLEDLCHEWKTVVIALVGIVG
                     IGVGCLTVGSMLFGREYALIAAPPIAGSTVAGIIVTTVAEAANRPELAAFVILVLSFQ
                     KFFGIPITTYCINKELKFKRDNGDFLKDESKSNFRLPSMRIFKEDLGKKSSTLVYLAK
                     LSFVAMIGNFVGLATLIPGFTPANYYLNPNIACLLVGLIFTRIGFLEKGILDKSQSNG
                     IIMFGCMLMLPGGLAKVTPTALFGMIVPTIGILVVGSVFIILFTAIVGKLLGYSPRMS
                     AAIGVTCMLAYPATQIISNEGIDAMEGTEEEKQRATDYLLPKMIIGGFVTVTIASVAF
                     ASIIGPIIFG"
     gene            6871..8250
                     /locus_tag="PC700_002330"
     CDS             6871..8250
                     /locus_tag="PC700_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2581634.1"
                     /translation="MIGSISINEIDKKRFKLDDLSKKIWENPEKAFKEFKACENTANF
                     LRSEGFDVEVGVGGLATAIRASFGSGKPVIGFMGEFDALPGLNQKVSTKQEAFELSAY
                     GHGCGHNLLCTAHVGAVVGLKREMLENNLSGTIVFYACPGEEQLTGKGFMARGGAFEG
                     LDLAINFHPNKISEATIGTSTAVNSVKFHFKGKTAHAGSDPQNGRSALDAVELTNVGA
                     NYLREHVTSDVRIHYTITDGGVAPNIVPDKASVWYYTRALSREAVESTYERLVKVAKG
                     AAMMTETEVEVEFLGGCYNTLNNHVLANLVSECMNEIKQEPWTREELDFAAELDRQSP
                     QQYKAMISKYNLPEGTHLYYGGGNVTNFNSYGSTDIGDVMHIVPTAYFFTGCTNLGAP
                     GHSWQFTSCAGSSIGEKGMIYASKIMAMFGAKILNNPEIAKKAKEEFDKSMNGKTYKC
                     PIPDDIPTP"
     gene            8356..9546
                     /locus_tag="PC700_002331"
     CDS             8356..9546
                     /locus_tag="PC700_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2581635.1"
                     /translation="MKNFFKEAQSIEGEIIRWRRYLHENPEIGCNLPKTVTFVTSTLE
                     KMGYSPKLTSNNGIVAVLDSENGGKTLLLRADMDALPIMEETDLPFKSKNKDASHSCG
                     HDNHTAMLLGAAKILMDNKHLLKGRVKFVFEPDEESTRGAKSMIDDGILENPQVDAAM
                     AFHSMVGKFLNTGQVAYSRGPAMASADIFEITVEGKATHGASPEFGVDPINIMSHIQI
                     ALNTILSREKPQKEPVVLTIGMFNAGNVPNAIPNKASMSGTLRTFDQFVREKLKNRII
                     EISTGIAKSFGGNAKVEFKMGTPAVINDLDVGDEIVSYVKEIIGNENVIDFPSAMGSE
                     DFSEILLRVPGVFLWIGMGSISQGYNFGMHNPKAIFNEEGLVYGSALFAHCAVKWLEN
                     NFSR"
     gene            complement(9674..10738)
                     /locus_tag="PC700_002332"
     CDS             complement(9674..10738)
                     /locus_tag="PC700_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581636.1"
                     /translation="MFLKLIFKYNTLDFSRAYFDLTDWADNPKDISYYLSNGTFGDLM
                     DSIDENASKMSLSDIAKLEKKIAKKHINLWNEGKIDFSTTRFSSIVPMLQNAGVNFHF
                     IYPDIDLLKNTFNNLIKDIHLHRMKGNQPSVIYITVKDTNYDDSTLDQVYKILNSIKK
                     DKLTDFIIEKNSNSFKIFTKCETIKSLTKNFTACYIKESIENSTNIKVCVGYGIGNDI
                     KQAMSSAISANKESKINPLNYSFLVNENYETIGPLLCDKLLTVSNKVTPHINTIAKSS
                     KLSTLTIQKIISVTEILQRDEVTPKELSSCLNVTVRTANRFLSSLLKSGNATILYEKQ
                     NSTKGRPERVYKLFISENKY"
     gene            complement(10886..>11290)
                     /locus_tag="PC700_002333"
     CDS             complement(10886..>11290)
                     /locus_tag="PC700_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2581637.1"
                     /translation="RLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNMIKNRRL
                     SRLISDVSWSEFIRQLEYKANWHGRKIVKVGKFFASSQICNKCGYKNEEVKDLNIREW
                     ICPSCNETHDRDINASINILKEGLRLITIQNK"
BASE COUNT         4167 a         1299 c         2012 g         3812 t
ORIGIN      
        1 tgattaaata aaactaaact taagaattta tttattaaca tacagttcat tatatttaaa
       61 atcattacta tgataattca taatagtaaa atagataatg gttcagacaa tattgtaaat
      121 attagtgaca gtaaaatagt atcaatttag tatataaact ttgccagata atcgcataac
      181 agagcttgta gttatagaat aaaaaaatat ctaggaaacc gttgacagat ttatatggat
      241 agtataacat atagaatatg aaattaaaca aaaaagaatt ttaattatgg cgacgtagtc
      301 ataattttga gtatgttact gattagcagg cagaactcaa gtatatatgt gaaaaataga
      361 gggatactct atacatatat gcttgagttc tttttattga aaggaggaat atatgaaaat
      421 aattcagatt ttaaataata atgtagcctt agtaaaaaga ggtaaaaatg aaattattgt
      481 atttgttaaa ggaattggct ttaaaaaaag agtgggacaa gtaataaatg aaaatgaaat
      541 agagagaaca tatatactag attcatacga tatgctagaa cactttagtt atctactaca
      601 aaactcagac tccaataata tagttcttat taatgaaata attgaatatg gtgagaaagc
      661 tttaaatata aagtcaagtg attatctaag cttaacctta ctagaccatt tagagttttt
      721 acttaaaaga tgtgaaaaga atcaatttat aaaaagtcct ttggtatgga atgttaaaag
      781 attttatcca aagcattttg aaattggaat gcatgcacta aaattaattg gtgatagtaa
      841 gggaattaat cttcctgatg atgaggcagt atcaatagca cttcattttg taaatatgga
      901 ggtaaataaa gagtctcatg atagtacaat tgtagagttg agaactttag cagatatagt
      961 ttctataata aaatatcatt ttaatgtaga gctagatgaa acttcaacaa attatatgag
     1021 atttactact catctacagt actttatcca aagaattatg tcagatgaag cttcaataga
     1081 tgaagataat tctatagatt tatataaaca agtaagtcaa ctttatccaa agtcatttgg
     1141 aagtgttcaa aagataagaa aatatataaa atcacaattt aatgtcgata tatctataag
     1201 tgaagaaact tatttaatgt tgcatataaa tagggtgact caaagactgg aggcaaaatc
     1261 agatgaaata taaagattta aatgaaaata taataaaact tataggggga aaagaaaata
     1321 taaagtcggt agcgcattgt gttactcgtt taagacttac tttaaatgat agaagtatcg
     1381 ctcaaacaga agaaattaaa aaattaaaag gagtaattga tgtagtttct aatgatgttg
     1441 cttatcaaat tataataggt actcatgttg tagatgtata taacgagttt atgtctatga
     1501 taggtttaag ttcaagtgaa tcaagtataa aaagtgaaag aaaaaaggga attagaggta
     1561 tcaaaggtat atttaattcc atctttgtgg tagtctcaga gactatgact tcagtaattg
     1621 aagtactact tgcagctggt atactcgcaa gtatattagc acttttaaat atgtctggca
     1681 tacttcagag tgatagccct acatatcaaa tattagatac attaagaagt gctacatttc
     1741 acttcttacc agtattaatt gcaatagcat cagcaaaaag acttaatgta aatccctata
     1801 tagcagtagt gttagcagtt acgttattat cgtcatcaat agatggtgta gaaggattaa
     1861 gtttatttgg tataaattta caggctacaa cttatgcaaa cagttttatt ccaatacttt
     1921 taggagtatg ggcaatggga attataattg aaggtttgaa aaaaatatta cctaaagcac
     1981 tacactattt tttagttcca gtgttatctt taataataac cctatcagtt acattaactg
     2041 tatttggacc tattggtatg tggattggtg atggattaaa ctatgcatgt atgtttttaa
     2101 ttgatacatt gggtaattgg agtgttgttg cactgtatgc agctttacaa ccactcttaa
     2161 tagtttttgg tgcagcaaac tttactatgc ctatatcttt aaattctgtg acaacattgg
     2221 ggtatgaccc aatttttatg ggcgctgcaa caatatctga tattgcagtt tgtggtgcaa
     2281 tgtttggata tttcttaaaa actaggaata aagaacaaaa acaattattt gggacagtaa
     2341 gttttagtgc acttatggga gttacagagc cttcagtgtt tggtgtgttt atgaaatata
     2401 gaagaccatt tgtagcagta atgataggtg gaggtatagg tggtttaata gcaggtttag
     2461 ctcaagttaa aacatatgga atggtttggg gattagctgc attaccaaca tatcttgttg
     2521 gaggagaggc atctaacttt acatttatga taataagtgt agtagtatca tttgtagcag
     2581 caacagcagc ttcctattta ctaggaatac cttcagatga aaaagaagaa gttaagggtg
     2641 ataatgaaaa agaattagaa ctaataatca acgagaacaa ttcaagaact attagtattg
     2701 gaaatgttgc aaaaggtcag gtaattgcac ttggggaagt taatgacaaa gcattttcct
     2761 ctggagcatt aggtaaaggg gttggagtta tacctagtga atcaaaatca actgttatat
     2821 ctcctgtaga tggaactata actatagtat ttccaactaa acatgcatat ggtatagtga
     2881 ctgatgaagg attagaaata ttaattcata ttggtataga tactgtaaac ttagatggaa
     2941 aattctttga aagtcttgta acacaaaatc aaagggttaa aaaaggagat gctttagcag
     3001 tatttgaatc tgataaaatt atagaggaag gctttgattc tactataata actgtagtta
     3061 caaatactag cgattattta gatgtaatct catcaaatga tgaatcagaa gaattattaa
     3121 ctgtaatagt ataaatttta ggagggaaaa ttcatgaata ttaaagatac atttttctgg
     3181 ggaggaagta ttgcagccca tcaatgtgaa ggttcatggg atagtgataa caaaggacct
     3241 gcaattatgg attttgtaac taagggctct tatgaaacgc caagagtaat tactgataaa
     3301 atagaagaaa aattggatta tccatctcat aatggaatag atttttataa tagatataaa
     3361 gaagatatag ctttatttaa agaaatggga tttagtgcat taagaatatc tatagattgg
     3421 tcaagaattt ttccaaatgg agatgatgaa aatcctaatg aattagggat taaatactat
     3481 gaaggtctga tagatacatt aatagaaaat aatatagaac ctattgtaac actatatcat
     3541 tttgaattac ctatgaattt agtacataaa tatggttcat ggaataacag aaaacttatg
     3601 gatttgtatt taaagtacag tgagactgta ataagaaggt ttgatgataa agtaaagtac
     3661 tggattacat ttaatgagat gaaccatatt gaccctcaaa cagagcactc ggatattttt
     3721 acttatatga tagcaggtat taagtatagt gaaatagaaa ataaggttca aacattggca
     3781 aatgtagggt ataatatgac tttggcgggt gtaaaggttg caaagttggc tagagaaatc
     3841 aatcccaaca ataaagttgg atgtgtattt ggactaaatc caatttatcc atacaattgt
     3901 aatcctagta atgtgttaaa tgcattttta gataatgatc gtgattatta tcaaattgat
     3961 gcaatgtgca atgggaagtt tccccaatat aaattaaaag aatatgaaaa aaataatata
     4021 actattgaag tgactgatga agacaaaaaa gcatttaaag aaggtaagtt agattttata
     4081 ggtcttaatt attatttttc aagtgtatct actccaaaag aaaatttatc aggtgataaa
     4141 tctttatttg gtgggattca aaatccatat ttggaacaaa gtaagtgggg atgggcaata
     4201 gaccctatag gtttaagatt taccttgaac tatatttaca gaaagtatca attacctatc
     4261 ttaattacag aaaatggttt aggggcatat gataaaatcg aagaagatgg aactataaat
     4321 gatgattata gaattgaata tttaagtaag catctatctc aaatgaagtc tgcaattgaa
     4381 gaggataatg ttgaatgttt tggatatttg atgtggggac caattgattt agtcagtgca
     4441 actacaggtc agatgagtaa gagatatgga tttatatatg ttgacttaga tgatgaaggc
     4501 aaaggaagtt ttaagagata taagaaaaaa tcatttgatt ggtacaaaga agtaataaaa
     4561 tctaatggag aagttttaga tttttctacg gatatagtag tagggtagta ataagataaa
     4621 tagaagttag gaactaatgt taattagtgt ctagtaaagc atttaaaata gagtataggg
     4681 tctttaaagt aaagtgtgta ttcttattaa aagtaatggc aactaaaatt actataaaaa
     4741 ggagaataca cattttttat gtttatactt aagtaaatat ttagacatat tcatagtata
     4801 atttactcat tatataaaaa tagtatgatt tactcactat ataactttat aaattttcta
     4861 gaaaaatgtc agaatatatt gacaaaaaaa agatttgaaa ttataatcta tattgagaat
     4921 agtttgacaa tataaaaaca agtattttta caaatagtga aattattttt attgtcaatt
     4981 taagaataat ttaggactac tataaagatg aaggtataga tgtcattata tgataaatca
     5041 tacttgtata gaaagaattt taaagtatgc ttatcattga tttaatataa taaataattt
     5101 ttgtttctag taatagttca taggaataag atatctaaat tcgcttagaa atagtctaat
     5161 ttacaaagga ggggatgcac tagtagataa aatttttata taaagaagac ttaaagtgga
     5221 gtatattctg ataatattgg tttaatggtt atattattat ttcgttttag atattttaag
     5281 aataatttaa gacgtttgta aaaatgtatt ctatatctaa tatttaaata aatataagtc
     5341 ttccaacatg tatactaaga tagaaagtag ttgaagtaca attctaactt gtaaaattat
     5401 gtagaaagta aaaatcaaaa agaaatctag taagttgata attatagtta atatttagag
     5461 agttaactct agttatattt atttagatat tttaggaata atttaagacg tatataatat
     5521 catcaaaaaa tatcaagcta taccatatgt agttaagaat aactgcataa tatacatttt
     5581 ataattattg ttttattgat aaaaaattat tatatttagg aggaaacaac aatggaaaat
     5641 ttttggaatc cacttacttg ctttactgta tgtgtaatga tttatgcttt tagtgagttt
     5701 ttatctaaga aaacaaaagg tgcaatttca acattgttat ttgtatgtat tatattttta
     5761 ctaggatttt ggacaaatgt tttaccaaaa gacattacag gagcatcagg gatattagct
     5821 gcaatgagca actttggtat agctttatta ataactaact taggaacact tataaatctt
     5881 gaagacttat gtcatgaatg gaaaacggtt gtaattgcat tagtaggtat tgtaggaata
     5941 ggggttggct gtttaacagt gggtagtatg ttatttggta gagagtatgc tttaatagca
     6001 gcacctccaa tagcaggttc tactgttgcc ggtattatcg taacaactgt tgcagaagct
     6061 gcaaatagac ctgaacttgc tgcatttgta attctagtat tatcatttca aaagttcttt
     6121 ggcattccga taactacata ttgtataaat aaagaattaa aatttaaaag agataacggg
     6181 gattttttga aagatgagtc aaaaagtaat tttagacttc caagtatgag aatttttaaa
     6241 gaagatttag gtaaaaaatc atctactcta gtgtatcttg caaagctttc atttgttgct
     6301 atgattggta attttgtagg attggctact ttaataccag gatttacccc tgcaaactat
     6361 tatttaaatc ctaatatagc atgtttgtta gtagggttga tttttacacg aataggtttt
     6421 ttagaaaaag gaattttaga taaatctcaa tctaatggaa ttataatgtt tggttgtatg
     6481 ttgatgttac ctggaggact tgctaaggta actccaacag cattgtttgg gatgattgtt
     6541 ccaactatag gaatacttgt tgtaggttct gtatttataa tattatttac tgcaattgta
     6601 ggaaaactat tgggatattc tccaagaatg tcagctgcaa ttggtgtaac ttgtatgcta
     6661 gcttatcctg caacacagat aatttccaac gaaggtattg atgctatgga aggaactgaa
     6721 gaagaaaaac aaagggcaac agattattta ttaccaaaga tgattatagg aggatttgtt
     6781 actgtaacca ttgcatcagt ggcatttgct agtataatag gtccaattat atttggttga
     6841 tagtaaatat acttatagga ggaataagaa atgataggaa gtatatctat aaatgagata
     6901 gataaaaaaa gattcaaact tgatgattta agtaagaaaa tatgggaaaa cccagaaaaa
     6961 gcatttaaag aatttaaagc atgtgaaaat acagcaaact ttctaagaag tgaaggattt
     7021 gatgtggaag taggtgtagg aggtttagcc acggctatta gagctagttt tggttcaggt
     7081 aaaccagtta taggttttat gggagaattt gatgcattac ctggtttaaa tcaaaaagtt
     7141 tcaacaaagc aagaagcatt tgaattgagt gcatatggtc atggatgtgg gcataattta
     7201 ttatgtacag cacatgtagg ggcagtagta ggacttaaaa gagagatgct agaaaataac
     7261 ttaagtggga ctatagtatt ttatgcatgt ccaggagaag aacaattaac aggtaaagga
     7321 tttatggcta gaggtggagc atttgaaggg ttagacttag ctataaattt tcatccaaat
     7381 aaaattagtg aagctacaat tggtacttca acagctgtta actctgtaaa atttcacttt
     7441 aagggaaaga ctgcacatgc aggctcagac cctcaaaatg gaagaagtgc actagatgct
     7501 gtggaactta ctaatgtggg agctaattat ttgagagaac atgttacttc tgatgtaagg
     7561 attcattata caattacaga tggaggagta gcacccaaca tagttcctga taaagcatca
     7621 gtatggtatt atacaagagc tttaagtaga gaagctgtag aaagtacata tgaaagactt
     7681 gtaaaggtag caaaaggagc tgcaatgatg actgaaactg aagttgaagt agagttttta
     7741 ggaggttgtt ataatacttt aaataatcat gttttagcaa atcttgtatc agaatgtatg
     7801 aatgagatta aacaagagcc ttggactcga gaggaattgg attttgcagc tgaattagat
     7861 agacaaagcc cacaacaata taaggctatg ataagtaaat ataatttgcc agaaggaact
     7921 catctatatt atggtggagg aaatgttaca aactttaata gttatggttc tactgatatt
     7981 ggagatgtaa tgcatatagt tccgacagca tactttttta cagggtgtac gaatctaggt
     8041 gcaccaggtc atagctggca gtttacatct tgtgcaggaa gttctatagg agaaaaaggt
     8101 atgatatatg catcaaaaat catggctatg tttggagcca aaatattaaa taatccagag
     8161 atagctaaaa aggcaaaaga agagtttgat aaatctatga atggtaaaac atataaatgt
     8221 cctattccag atgatattcc aactccttaa aaagacatga agtttaatat ataaaattat
     8281 aatttttata tcagcatcat ttcattacta aaagaaagtg agattttgct gactatctga
     8341 ttaggaggaa ggaatatgaa aaattttttt aaagaagcgc agtcaataga aggggaaatt
     8401 attagatgga gaagatattt acatgaaaat cccgaaattg gttgcaatct accgaaaaca
     8461 gtaacctttg ttaccagtac tttagaaaaa atgggatatt ctccgaaatt gacgtctaat
     8521 aatggaatag tagctgtttt agacagtgaa aatggtggaa agaccttatt attaagggca
     8581 gatatggatg cacttcctat tatggaggag acggatttac cttttaaaag taagaataaa
     8641 gatgcatctc atagttgtgg acacgataat cacacagcaa tgctcttagg agcagctaaa
     8701 atacttatgg ataataagca cttacttaaa ggtagagtaa agtttgtatt tgaaccagat
     8761 gaagagagta ccagaggtgc aaaatcaatg attgatgatg gtatattaga aaatcctcag
     8821 gtcgatgcag ctatggcatt tcacagtatg gtgggtaaat ttcttaacac aggtcaagta
     8881 gcttattcac gtggtccagc tatggcatct gcggatatat ttgaaataac agtagaagga
     8941 aaagcaactc atggagcaag ccctgaattt ggagtggacc caataaacat aatgtcgcat
     9001 atacaaatag cattaaacac tatactttca agagaaaaac ctcagaaaga gcctgtagtt
     9061 ttaactattg gtatgtttaa tgcaggaaac gtaccaaatg ctataccaaa taaagcatct
     9121 atgtcaggaa ctttaagaac atttgaccaa tttgttagag aaaaattaaa aaatagaatt
     9181 attgaaatat ctacaggaat agctaaatca tttggaggaa atgctaaagt ggaatttaaa
     9241 atggggactc ccgcagttat aaatgatttg gatgtaggag atgagattgt atcttatgtt
     9301 aaagaaatta taggaaatga gaatgtaata gactttccat ctgcaatggg ttcagaagat
     9361 ttttcagaga ttttactaag ggttccagga gtatttttat ggattggaat gggaagtatt
     9421 agtcagggat ataattttgg tatgcataat cctaaagcaa tttttaatga agagggatta
     9481 gtttatggtt cagctttatt tgctcattgt gcagtaaaat ggttagaaaa taattttagt
     9541 agataataac ataaaatatt attaaatatc ataaaaggta tatcttaaaa agatgcaaag
     9601 atataccttt tagaaagtca tatataattt tacttaatat tagatgataa aatattttta
     9661 tgcattttgg attttaatat ttattttctg atataaataa tttgtatacc ctttcaggtc
     9721 taccttttgt tgagttttgt ttttcatata atatagtagc gtttccagac tttaagagac
     9781 tacttagaaa tctattagca gttctaactg ttacatttaa acatgaagat aattccttag
     9841 gagtaacttc atctctttgt agaatttcag taacagatat aatcttttga atagtaagag
     9901 tagacaattt agaacttttg gcaattgtat ttatatgtgg agttacttta tttgaaacag
     9961 taagtaactt atcacacagt aaaggtccta ttgtctcata attttcgttg actaagaaac
    10021 tgtaatttaa aggatttatt ttagattcct tattagcact tatggcacta gacatagctt
    10081 gctttatatc attaccaatt ccatatccaa cacatacttt aatatttgta ctattttcaa
    10141 tagattcttt tatataacat gcagtaaaat ttttagttaa tgatttaatc gtttcacatt
    10201 tagtgaaaat tttaaaagaa ttactatttt tttcaattat aaaatcggtt aatttatctt
    10261 ttttaatact atttaatatt ttataaactt ggtctagagt agagtcatca taatttgtat
    10321 ctttaactgt tatatatatt acacttggtt gatttccctt cattctatgt aaatgaatat
    10381 cttttattaa gttattaaaa gtatttttta ataaatctat atcagggtat atgaaatgaa
    10441 agtttactcc tgcattttgt aacataggaa caatactact aaaacgagta gtagaaaaat
    10501 cgattttccc ttcattccat agattaatat gtttttttgc aatctttttt tcaagttttg
    10561 ctatgtcaga taaactcatt ttagatgcat tttcatcaat actatccatt aagtctccaa
    10621 atgtgccatt tgataaataa tatgatatat cttttggatt atctgcccag tctgttaagt
    10681 caaagtatgc tctagaaaaa tctaatgtat tatatttaaa gataagcttt agaaacatat
    10741 catagtaact ttgtaagtca gtaccaaact gaacctccca tgactaaagt cacagggttc
    10801 ctgcttcata gaattttgca tactaaaata tgtcttacta attcttcaca ggccatcccc
    10861 gtagttccta cggttcttac ataagttatt tattttgaat tgttattagt cttaatcctt
    10921 cttttaatat atttatactt gcatttatat ctctatcgtg agtttcatta caactaggac
    10981 aaatccattc tcttatattt aagtccttca cttcctcgtt tttataccca catttattgc
    11041 atatttgtga acttgcaaag aatttaccta cttttacaat ttttctacca tgccaattag
    11101 ccttgtattc taattgacga ataaactctg accatgatac atcagaaatt aagcgagata
    11161 gtctacgatt tttaatcata ttctttactt gtaaatcttc tatgcaaata atatcattct
    11221 cttttattaa tttagtagat aatttttgta gaaaatcatt tctttgatta gctatatgtt
    11281 cttgaagtct
//
LOCUS       DAMJWP010000111        11269 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.116, whole genome shotgun sequence.
ACCESSION   DAMJWP010000111 DAMJWP010000000
VERSION     DAMJWP010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11269
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.116"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            217..1392
                     /locus_tag="PC700_002334"
     CDS             217..1392
                     /locus_tag="PC700_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2581638.1"
                     /translation="MNLEKIKKFFGFPLDKKHKENVEKELCNYLYHTGKIICIIIVIS
                     QIIMMISISAREGGPFATPRRQWYFYMYVTLFCVSSVFMFVFSYLWNKKKDCCTFYIN
                     LWIIYVTFFCLWGCVITLLDQLGGNDLSVFSYIMLAAAAFSVLKFGESVIVFGGCFLF
                     LNLFLPGIQTIPGNEFSNIVNTFFITSLAIFVSTMLYRNRVFTYYDKLLISQQYEEIS
                     HINNLLNDKVMTDDLTKMNNRRYLEDILQKKLISHANQEPITGMMIDIDYFKQFNDNY
                     GHQKGDYCLQNIAKVILDFTSSENEHAIRYGGEEFFICIFNCDETDAKLKSECLRKII
                     ESCNFERDDVPLKCVTVSIGVYTKKIEKAITLNEFISNCDKALYTAKEQGRNCVAFY"
     gene            complement(1565..2962)
                     /gene="asnS"
                     /locus_tag="PC700_002335"
     CDS             complement(1565..2962)
                     /gene="asnS"
                     /locus_tag="PC700_002335"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDF2581639.1"
                     /translation="MQKEFITVKELYREKEQYIGKTVKVAGWIRTSRVSKNFGFIELN
                     DGSFFKNMQIVFDEKVENFKEVGKLAISSSILVEGELVSTEGAKQPVEIHAKSIVVEG
                     ESDSSYPLQKKRHTLEYLRTIAHLRPRSNTFSAVFRVRSIAAYAIHKFFQERNFVYAH
                     SPIITGSDCEGAGEMFRITTMDLNDIPKTEDGKIDYTQDFFGKEANLTVSGQLNAEIM
                     ALSFRNVYTFGPTFRAENSYTGRHASEFWMIEPEIVFADLEDNMELAEDMIKYVISYV
                     LENAPEEMEFFNSFIDKGLLERLNKIVNSEFIRITYTKAVELLIESGHEFEYPVEWGC
                     DLQTEHERYITEQIYNCPVFVTDYPKDIKAFYMRMNEDGKTVRAMDLLVPGVGEIVGG
                     SQREEREDVLLDRINEMGLREEDYWWYLELRKFGTATHSGFGLGFERIIMYMTGMSNI
                     RDVIPFPRTPKNAEF"
     misc_binding    complement(3039..3272)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(3441..3635)
                     /locus_tag="PC700_002336"
     CDS             complement(3441..3635)
                     /locus_tag="PC700_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HDF2581640.1"
                     /translation="MESKKARALKGKDNKRLKSSLLVGNESTAAWADVEKLKPHSKVS
                     IPSIKGVEEAKDWVDNGSKL"
     gene            complement(3909..5582)
                     /locus_tag="PC700_002337"
     CDS             complement(3909..5582)
                     /locus_tag="PC700_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HDF2581641.1"
                     /translation="MEKSYCIIYQGDIESALQENGINRYMVLNSQLAVIYVPVDFDET
                     ILNNIIQVAWWEESEPMSSLIEITNNVNNGETITTAAETDYIYENPYNDITGRGILLA
                     VIDSGIDYLHPDFINDDGTSKVLYLWDQEANTNPPPEGFIFGSEFTRSQLNIAINRND
                     GSLSQDNIGTGTLVSGILAGNGRINSQYRGITTESDLIVVKLKSYTDTYYAGRINYSV
                     SDFLAAITYVTNIARTENKPLIINLTIGVKSSAVATTSILDTFNILSSAGVVVVSGAG
                     NQGNTDIHYSGRFSSVGEVQDVIIQDGDDYALDITLNTNGPDKVGAQIISPSGEVSHD
                     IRYSPDFYIYRGKFNLENTTYAMRFIYPYITSGKENLEIRLRDIKPGVWILRLTSELI
                     ISGEYDIYLPNKNLIAPDTRFLDPDSVATITMYAASDDVITVGTFNNKTDSMWIGSSK
                     GPIRGRGIKPDIVASGVDIISTYKNGTYNTGTGTGVSSSIVTGVLALLMEYLEKQDNV
                     PRLSLFTQVLKTYLILGATKLEIYTYPNVSQGYGILNLKNTIQQIANTL"
     gene            complement(5592..8990)
                     /locus_tag="PC700_002338"
     CDS             complement(5592..8990)
                     /locus_tag="PC700_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDF2581642.1"
                     /translation="MIIINYELIVKYNGDILRLEEELGVSVEILNSSYAIITSSNEED
                     VNTLLTYPEIEFIEKPFILQTQDVQSFSSTGITGFKNRTGLTGKGTIIGIIDSGIDYT
                     LPVFRDSDGRSKILYYWDQSIQGNPPEGFREGTLYTNEDINNAIDGSMYIPISTTSLH
                     GTHVAGICATIASDARIIVVRVGNIQTDIFSRSTEFMRAIKFILDRALELRMPVTLNI
                     SYGSNEGSHRGTSLFEQYIDDMCLFWKNNIVVAAGNNADKGGHKRIRLQNNITEEVEF
                     IVGEGERILNINIWPDFVDDFSVHLVNPSNNQTQAISLTSGEIRNTLGETRITGYFYP
                     IAPYSLTRRVTLQLSSNTQITPGLWKIVFEPIDIVTGNVNIYLPTSEGLNRNTRFLIP
                     TQELTVTVPGTASRVVTVGSFNSRTDIVSIFSGEGDTQLGVFKPDLLAPGEDIISFLP
                     GGTSGALTGTSMATPHVTGVCSLFMEWGIVNGNDLFLYSQKLRALLLKGARRLSNQSY
                     PNNSSGFGFLNLSDIDLYTLSSINQDLETEDIGYRSINKSFKDEENRYKFIDGYNIQI
                     HNDLENEIYISKNASRQSGILSGIDVVHTPEFEEELAGLGMSQNFFKISDSLGVLSIN
                     NTDYSSIQRVLQLPSIIRTVSTTKMTLLGEINRGTFGGVVATEEMGVNFFKNNPNINI
                     TGRGTLISIADTGIDYLHPDFIYPDGTSKIVYLWDQTKEGTPPDGFYIGTEYTREDIN
                     RAIAENDPSLSQDEVGQGTMLSGICSGLGNVNSEYAGIAEDSELIIIKLGKIDGFYNS
                     AMLFAASQYAYKKAFELRRPLVINMSLGTSSLAGLTNRSNSEKAFFTRGLCITAGAGN
                     EGNTQTHTSGIIPHVGGSVEVELELNEDEEELSLELWLNRPDKADVIIVSPTGEESKS
                     VGISNYNKVTGLFDLEGTEYSITYIYPTTFSGQQFTNVTLKNAKRGVWKIRLVGVYII
                     TGRYNLYLPNRELLKSGTRFREVDPFYTINYPAIQDDLITVGAYNTINGSLWQSSSRG
                     PTIEDRLKPDIVAPGVNIIAAYPGNTYATITGTAAASAHAAGAAAMYFQYTFVDGRYP
                     NQAYVQKIKTFMQAGARKDSNTVYPNTNSGYGLLDVRGMFDVLR"
     gene            complement(9156..10352)
                     /locus_tag="PC700_002339"
     CDS             complement(9156..10352)
                     /locus_tag="PC700_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDF2581643.1"
                     /translation="MFIEFFYLLRSRGLNISLNEWMTLIEALDKGLCYSNFSNFYYLC
                     RMILIKSESDFDKFDAVFLEYFKGIEHQEEIPEEIMNWLKKPDIDLEEFERLEQNPNI
                     NLDKLRAKLEERIKEQDSPHNGGNYWIGTGGTSELGHSGKGQTGIRIGGNSTYGRAVL
                     EVAGERKYRDFRDDEVLNMRQFQVALKRLRQFSTRIDAPKTELDLDKTIEETCNNAGY
                     LKLFFEKPRKNTVKLLLLMDSGGSMRGYSTLCNTLFQSVSKSNHFKDVKIYYFHNCIY
                     DRLFTTPECWLSKSINTEWILKNIDKNYKVIIVGDASMSPSELLHIGGNYRGPYNYTP
                     GIEWLKRFKRKYSKIVWMNPELRDGWDSINYWYQTQRMIQSEFNMFPLTVKGLEKALK
                     NLMLSR"
     gene            complement(10373..11194)
                     /locus_tag="PC700_002340"
     CDS             complement(10373..11194)
                     /locus_tag="PC700_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDF2581644.1"
                     /translation="MIKKEISNFRGSSDYVVSPELMASVNVAIALEKPLLIKGEPGTG
                     KTMLAQAISNELKKDLVIWNIKSTTKAQEGLYVYDTVQRLYDSQFGGEGVDDISKYIK
                     YGKLGEAFSSNQQVILLIDEIDKADLEFPNDLLWELDKMEFYINETKETVRAKQRPIV
                     IITSNAEKELPDAFLRRCIFHYIEFPDRDMMEEIVKVHFDKVEEHLLEQVMTTFYWIR
                     SLKDIQKKPSTSELIDWIQALTLSGMPIEKIEKEVPFAGILLKNNEDIESMQRHL"
BASE COUNT         3596 a         2060 c         1309 g         4304 t
ORIGIN      
        1 tttattttca gcagcaaact atctgtaaat cttaattata gtaaagtatt aattaataaa
       61 gttactcaac attagctaat aaattttatc tatcacaatt ttagattgtt acacacaata
      121 tattttgata taattaaatc ataaacgaag tattccaaat atctaatgat agaaaggaat
      181 acttattttt atgctttatt tttaatggag gcttatatga acttagaaaa aataaaaaaa
      241 ttttttggat ttcctctaga caaaaaacac aaagaaaatg ttgaaaaaga actctgcaat
      301 tatctttatc atacgggtaa aatcatttgc ataataatag taatatctca aataattatg
      361 atgatatcaa tctcagctag agaaggaggc ccttttgcca caccaagacg ccaatggtat
      421 ttttatatgt atgtaacact attttgtgta tcttctgtct ttatgtttgt attttcttat
      481 ctttggaata aaaaaaagga ttgttgtacg ttttatataa atttatggat aatttatgtg
      541 acttttttct gcctatgggg ttgtgtaatt actctacttg accagttagg tggaaatgac
      601 ctaagtgtat ttagttatat tatgttagca gctgcagctt tctcagtatt aaaatttgga
      661 gaatcagtaa tagtttttgg aggatgcttt ttgtttctta atttattctt acctgggatt
      721 caaactatac caggcaatga gtttagtaat attgtcaaca cattttttat aacatccctt
      781 gccatttttg tttctacaat gctatataga aatcgtgtat ttacttacta tgataaactt
      841 ttaattagtc aacaatatga agaaatatct catataaata atttacttaa cgacaaagtt
      901 atgacagacg accttactaa aatgaacaac cgtagatacc ttgaagatat tctacaaaaa
      961 aaattaatca gccatgcaaa tcaagaacct ataactggaa tgatgataga tatagattat
     1021 ttcaaacaat ttaatgataa ttatggacat caaaagggag actattgtct acaaaatatt
     1081 gccaaagtaa ttttagattt tacaagttct gaaaatgaac atgctatacg atatggtggt
     1141 gaggaatttt ttatatgtat ttttaactgt gatgagacag atgctaaact taaatcagaa
     1201 tgtttacgaa aaattataga gtcctgcaat tttgagcgtg atgatgttcc tttaaaatgt
     1261 gtaactgtta gtattggagt ttatacaaag aaaattgaaa aggcaataac attaaatgag
     1321 tttataagta attgtgataa ggctctatat actgcaaaag aacagggacg taattgtgtt
     1381 gcattttatt aaaaagttac atactattat ataaacctat aagtattata tcgcaaaata
     1441 aaagtaatca aattatatat ttatagtaca ataaaaataa cttgcatatc tctaaaatag
     1501 ataaatatgc aagtcatttt agtgtttata cttaaagtta tataagtttt attatatata
     1561 attattaaaa ttcagcattt ttaggagttc ttgggaaagg tataacgtct cttatgttag
     1621 acatacctgt catatacata attattcttt caaatccaag accaaatcca gagtgagtag
     1681 cagtaccaaa ctttctaagt tctaaatacc accaataatc ttcttctctt aatcccattt
     1741 cattaattct atctaacaat acatcttctc tttcttctct ttgagaacct cctacgattt
     1801 ctccaactcc aggaactaat aaatccattg ctctaacagt tttaccgtct tcattcattc
     1861 tcatatagaa agcttttata tcttttggat aatctgtaac aaacactgga caattatata
     1921 tttgctccgt tatatatctt tcatgctcag tttgaaggtc acatccccac tcaacaggat
     1981 actcaaattc atgaccagat tcaattaata attccactgc tttagtatac gttattctaa
     2041 taaactcaga gtttactatc ttatttaatc tttctaacaa tcctttatct ataaaactat
     2101 taaaaaattc catttcttca ggagcatttt ctaatacata gcttataaca tattttatca
     2161 tatcttctgc aagttccatg ttatcttcaa ggtctgcaaa aactatttct ggctctatca
     2221 tccaaaattc tgaagcatgt cttcctgtat aagagttttc agctctaaat gtaggtccaa
     2281 aagtataaac gtttctaaaa gaaagtgcca ttatctcggc atttagttga ccactaactg
     2341 ttaaattagc ctcttttcca aagaaatctt gagtatagtc tattttacca tcttcagttt
     2401 ttggaatatc atttaaatcc atagttgtta ttctaaacat ttctcctgca ccttcacagt
     2461 cactacccgt tatgattgga gaatgtgcat atacaaagtt tctttcttgg aagaatttgt
     2521 gtattgcata ggcagctatt gatcttactc taaatacagc tgaaaaagta ttacttcttg
     2581 gtcttaaatg tgctatcgtt cttaaatatt caagcgtatg tctcttcttt tgtagtggat
     2641 acgaactatc tgattcccct tctacaacta tgctttttgc atgtatttcg actggttgtt
     2701 tagccccttc tgtagatact aactctccct ctacaagtat agatgagctt attgctaatt
     2761 ttcctacctc tttaaaattt tctactttct catcaaatac tatctgcata tttttaaaga
     2821 agcttccatc atttagctct ataaatccaa aatttttaga tactctagat gttcttatcc
     2881 atccagctac ttttacagtc ttccctatgt actgttcttt ttctctgtac aattctttta
     2941 ctgttataaa ttccttttgc attttaaatc ctcctagtat aattgttatt aatttctttt
     3001 aaatataatt tcaataaaaa aagcccttca tccctatata aagggacgaa agactattac
     3061 tctcgcggta ccaccctagt tgtcataatg acctcttagt taagcaatgc ttaaagccta
     3121 taacggtgcc ttccgtctaa gtctacttac taaataagct ttcgattaga aactccaaga
     3181 tgttcttcaa tataaacctc gtattgagct ttcaccatcc tcaactcgct agaactacat
     3241 tttatatcta ctcttctctt cacagtattt atcttttatt atttctacta attaacaaaa
     3301 ttataccaat ttataaattt ttagtcaata aatttatata attgatactg attgttattt
     3361 aaaatataat aattctacct tgcataatga taataaatac ttatcttcat tacttcttta
     3421 ttacttaaat ttccaaaatt ttataattta cttccattgt caacccagtc ttttgcttct
     3481 tcgacaccct taattgaagg tattgaaact ttactatgag gttttaattt ttcaacatca
     3541 gcccatgctg ctgtcgactc atttccaact aaaagtgaac ttttaagtct tttattatct
     3601 ttacctttta atgctcttgc ctttttactt tccaaaacta tcacctctat tatttatttt
     3661 gtaatagttt tagcaatttt acaaataaaa ttcataagtc taaattataa tgatttttaa
     3721 attaacatca tatcttttca atacttaatt tatacctatt tatatacaaa atatttactt
     3781 tgtgtattca aaatataaaa ctgttttttt aattttcata tttatttttt atgccatttt
     3841 tctactctat ctaaagtatt tgatacaatt aaattactaa tcaattataa ttttacatag
     3901 gttcttatct atagagtatt tgctatctgt tgaatcgtat tttttaaatt taaaatccca
     3961 tatccctgcg atacatttgg atatgtatat atctcaagtt ttgtagcacc caatattaaa
     4021 tatgttttta aaacttgtgt aaacaatgat aatctcggta cattatcctg tttttctaag
     4081 tattccatca ataaggctaa aacacctgtg actatagaac tgctcactcc tgttcctgtt
     4141 cctgtattat atgttccatt tttatatgtg gatattatat ctacacctga agctactata
     4201 tctggcttaa ttcctcttcc tctaattggt cctttcgaag aaccaatcca catactatca
     4261 gtcttattat taaatgtacc aactgtaatt acatcatcac tagcagcata catagttata
     4321 gtagctactg aatcagggtc taaaaacctt gtgtctggag ctatcaaatt tttatttggt
     4381 aagtatatgt catattctcc acttattata agttctgaag tcaatcttaa aatccatact
     4441 ccaggtttta tatctcttaa tcttatctcc aaattttctt ttccagaggt aatatatgga
     4501 taaataaatc tcattgcata agtagtattt tctaagttaa attttcctct atatatataa
     4561 aagtctggag aataccttat atcatgacta acttcacctg aaggagatat tatctgtgct
     4621 cccactttat ctggaccatt cgtatttagt gttatatcaa gagcatagtc atctccatct
     4681 tgtattatta catcttgaac ttctcccaca ctgctaaatc tgccagaata atgaatatct
     4741 gtattccctt gatttcctgc accactcact actaccacac cagcactact caatatatta
     4801 aaagtatcta atattgaagt agttgctaca gcacttgact taactccaat agtcaaattt
     4861 attattagtg gtttattttc tgttcttgct atattagtaa catacgttat agctgctaaa
     4921 aaatctgaaa cactataatt tatccttcca gcataataag tatctgtata tgattttaat
     4981 ttaactacta ttaagtctga ttctgtagta atacctctat actgcgaatt tatccttcca
     5041 tttccagcta atataccaga aactaaagtt ccagtaccta tattatcttg actcaaactt
     5101 ccatcatttc tatttatggc tatatttaac tgacttcttg taaattcact tccaaaaata
     5161 aaaccttctg gaggtggatt tgtatttgct tcttggtccc ataaatatag aactttacta
     5221 gttccatcat catttataaa atcaggatgc aaataatcta taccagaatc tataactgct
     5281 aataaaattc ctcttcctgt tatatcatta tatggatttt cataaatata atcagtttca
     5341 gctgctgttg taatggtttc tccattattt acattattag ttatttcaat tagactgctc
     5401 attggttcag attcttccca ccaagcaact tgtattatat tatttaaaat agtttcatca
     5461 aaatctacag gtacatatat tactgcaagc tgactattta aaaccatata cctatttatt
     5521 ccattttctt gtagggcaga ttctatatca ccttgataaa ttatacaata agatttttcc
     5581 ataaatccct cctatcttaa aacatcaaac attcctctaa catccaaaag cccatatcca
     5641 gaatttgtat ttggatatac tgtgtttgaa tcttttctag caccagcttg cataaaagtt
     5701 tttatttttt gcacatatgc ttggtttgga tatctaccat caacaaaagt atattggaaa
     5761 tacattgcag cagcaccagc tgcatgtgca cttgctgctg ctgtaccagt tatagttgca
     5821 tacgtgttcc caggataagc agctattata ttgacacctg gtgctactat atctggtttt
     5881 aacctgtctt caatagttgg tcctcttgat gaactttgcc ataaactacc attaatagta
     5941 ttgtaggctc ctacagttat taaatcatct tgaatagcag gataatttat agtgtaaaaa
     6001 gggtctacct ctctaaatct agttccactt tttaaaagtt ctctatttgg aaggtataag
     6061 ttatatcttc ctgttataat ataaacccct actaatcgaa tcttccatac acctcttttt
     6121 gcattcttta aagtaacatt agtaaattgc tgcccagaaa aagttgttgg gtatatatac
     6181 gttatagaat attcagtccc ctctaaatca aataaacctg ttactttatt ataatttgaa
     6241 attccaacac ttttactttc ttcaccagtg ggagatacta ttattacatc agctttatct
     6301 ggtctattta accaaagttc taatgataat tcttcctcgt cttcatttaa ctctaattca
     6361 acttcaacag aacctccaac atgtggtatt attccagatg tatgtgtctg tgtattgccc
     6421 tcatttcctg ctcctgctgt tatacataac cctctagtaa agaatgcttt ttcactattg
     6481 ctcctattgg ttaaacctgc caagctactg gtacctaaag acatatttat aactagtggt
     6541 cttctaagtt caaaagcttt tttatatgca tactgagatg cagcaaacaa cattgcacta
     6601 ttataaaaac catctatctt accgagtttt attattatta gctcagaatc ttctgctata
     6661 cctgcatatt cactatttac atttccaagc cctgagcata taccactcaa catagttcct
     6721 tgaccaactt catcctgaga tagactaggg tcattttctg cgatagctcg atttatatct
     6781 tctcgtgtat attctgtccc aatataaaaa ccatcaggtg gagttccttc ttttgtttgg
     6841 tcccataaat atactatttt tgaagtacca tctggataaa taaaatctgg atgtagatag
     6901 tctattcctg tatctgcaat tgatattaga gtccctcttc ctgttatatt aatattagga
     6961 ttatttttaa agaaatttac gcccatttct tctgttgcaa ctacacctcc aaaagtacct
     7021 cgattaattt ctccaagtaa tgtcatcttt gtagtcgata cagttcttat gattgaagga
     7081 agttgcaaaa ctcgttgtat actactatag tctgtattat ttatagatag tacacctaat
     7141 gaatcactta tcttaaaaaa attttgactc atacctaacc cagctagttc ttcttcaaat
     7201 tcgggagtat ggactacatc tattcctgat agtattcctg attgtctact tgcattttta
     7261 cttatataaa tttcattctc taagtcatta tgtatttgta tattataacc atctataaac
     7321 ttatacctat tttcctcatc tttaaatgac ttattaatac ttctatatcc aatatcctcc
     7381 gtttctaaat cttgatttat actagataga gtgtacaaat ctatatctga taaatttaaa
     7441 aaaccaaatc ctgatgaatt atttggataa gattgattac ttaatctcct tgctcccttt
     7501 aaaagtaatg cccttaattt ctgtgaatac aaaaataaat catttccatt tacaattccc
     7561 cattccataa acaatgaaca aacccctgta acatgaggtg tagccatgct agttccagtt
     7621 aatgctccac ttgtacctcc tggtaaaaaa gatattatat cttcacctgg agctaataaa
     7681 tcaggtttaa aaacacctaa ttgtgtatct ccttcaccag aaaatataga tactatgtct
     7741 gttcttgaat taaaactacc tactgttaca actctacttg ctgttccagg aactgttaca
     7801 gtaagctctt gagtaggaat cagaaatcta gtatttctat ttagtccttc agaagttggc
     7861 aaatatatat ttacattacc agttactata tctattggtt caaatacaat tttccataac
     7921 ccaggagtta tctgtgtatt tgaacttaat tgtagagtaa ctcttcttgt aagagagtac
     7981 ggtgctatag ggtaaaaata accagttatt ctagtttcac ccaaagtatt tcttatctca
     8041 cctgaagtta gagagattgc ttgtgtttga ttgtttgatg ggtttaccaa atgtacacta
     8101 aaatcatcaa caaaatctgg ccatatattt atgtttaata tacgctctcc ttccccaact
     8161 ataaactcaa cttcttctgt aatattattt tgaagcctaa tccttttatg acctccttta
     8221 tctgcattat ttccagctgc tacaactata ttgtttttcc aaaataaaca catatcatct
     8281 atatattgtt caaataaaga tgttcctcta tgtgaacctt cattacttcc ataacttata
     8341 tttaatgtaa caggcattct taattctaat gccctatcta aaataaattt aattgccctc
     8401 ataaactctg tacttctcga aaaaatatcg gtctgtatat ttccaactct aaccactatt
     8461 attctggcat cacttgcaat agttgcacaa atacctgcaa catgtgtccc atgtaaacta
     8521 gttgttgaaa taggtatata catacttcca tctattgcat tatttatatc ttcattagta
     8581 tataatgttc cttctctaaa accttctggt ggatttcctt gaatagattg gtcccaataa
     8641 tataatatct tagacctccc atcactatct ctaaacacag gtagagtata atctattccc
     8701 gaatcaatta taccaattat agttcccttt ccagttaaac cagttctatt tttaaacccc
     8761 gttattcctg tacttgaaaa actttgcaca tcctgagtct gtaatataaa aggcttttct
     8821 ataaattcaa tttctggata agttaaaagt gtatttacat cttcttcatt acttgatgtt
     8881 attattgcat atgatgaatt aagtatctct actgatacac ctaattcttc ttctaatctc
     8941 aatatatctc cattgtattt tacaattaat tcataattta ttataatcac ctcgacttct
     9001 aaaattatta ttaatgtata tgttttgtgt aagtctaaaa tgtattagac tatataattt
     9061 tttaagtttc agttatacaa tttataatag atatattaaa aaataaataa tgatggtaca
     9121 attttgaata aatcatacca tcatttatat ttactttatc ttgaaagcat taaatttttc
     9181 aatgcttttt caagaccttt tacagtcagt ggaaacatat taaattcact ttgtatcatt
     9241 ctttgagttt ggtaccaata attaattgaa tcccatccat ctctaagttc tggattcatc
     9301 catacaattt tactgtactt tctcttaaac cttttaagcc attctatacc aggtgtgtaa
     9361 ttatatggac cacgatagtt accaccaata tgaagtaact cagaaggcga catagaagca
     9421 tctccaacaa ttataacttt atagttttta tctatatttt ttaatatcca ttcagtatta
     9481 atagatttac ttaaccaaca ttcaggtgtt gtaaataatc tatcataaat acagttatgg
     9541 aaataatata tctttacatc tttaaaatga tttgatttac ttacagactg aaataatgta
     9601 ttacataaag tgctataccc cctcattgaa ccacctgaat ccataagcaa taaaagcttc
     9661 actgtatttt ttcttggctt ctcaaaaaat aactttaaat atcctgcatt attacaagtc
     9721 tcttcaatag tcttatccaa atcaagctct gtctttggag catctattct agtagaaaat
     9781 tgacgaagtc tctttagtgc aacttgaaat tgacgcatat ttaaaacttc atcatctcta
     9841 aaatctctgt attttctttc tcctgcaact tcaagaacag ctctaccata agtagaattt
     9901 ccacctatac gtataccagt ttgccctttt cctgaatgtc ctaactcgga agtgcctcct
     9961 gtgcctatcc aataattacc accattatga ggagagtctt gttcttttat tcgttcctcc
    10021 aatttagctc ttaatttatc aagatttata tttggatttt gttccaatct ttcaaactct
    10081 tctaaatcta tatctggttt tttaagccaa ttcataatct cttctggaat ttcttcttgg
    10141 tgttctatac ctttaaaata ctccaaaaac actgcatcaa acttatcaaa atcactttca
    10201 ctttttatta aaatcatcct gcaaagatag taaaaatttg aaaagtttga atagcacagc
    10261 cctttatcca atgcttcaat taaagtcatc cattcattta aagatatatt taatcctctt
    10321 gacctcaata aataaaaaaa ttcaataaac ataattatcc agctcctatt aactataagt
    10381 gtctttgcat agattcaata tcctcattat tcttaagtag tattcctgca aaaggaactt
    10441 ctttttctat tttttcaata ggcattccac tcaaagttaa tgcttgtatc cagtctatta
    10501 actctgatgt actaggcttt ttttgtatgt cctttaaact tctaatccaa taaaaagtag
    10561 tcattacttg ttccaatagg tgttcttcta ctttatcaaa atgtacttta actatttctt
    10621 ccatcatgtc tctatcagga aattctatat aatgaaatat acatcttctt aaaaaagcat
    10681 ctggaagttc tttttccgca tttgaagtta taataactat tggtctttgt ttagctctta
    10741 cagtttcttt tgtctcattt atgtaaaact ccattttatc caattcccaa agcaagtcat
    10801 ttggaaattc taaatccgcc ttatctattt catctattaa aagaattact tgttgatttg
    10861 agctaaaggc ctctcctagt ttaccatatt ttatgtactt gctaatatca tctacacctt
    10921 ctcctccaaa ttggctatca taaagtcttt gaacagtatc atatacataa agcccttctt
    10981 gtgcttttgt agttgatttt atattccaaa ttactaaatc tttttttaat tcatttgaaa
    11041 ttgcttgtgc tagcatagtt ttacctgttc caggttcgcc cttaatcaaa agtggctttt
    11101 caagagcaat agcaacatta actgatgcca tcaattctgg tgatactaca taatcactac
    11161 ttcctctaaa gttagatatc tcttttttta tcataaatcc tactcctttt aaacagtatt
    11221 ttctaaatgt atatagactt tattactatt tttgatataa aaataataa
//
LOCUS       DAMJWP010000112        11204 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.117, whole genome shotgun sequence.
ACCESSION   DAMJWP010000112 DAMJWP010000000
VERSION     DAMJWP010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11204
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.117"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(72..497)
                     /locus_tag="PC700_002341"
                     /pseudo
     CDS             complement(72..497)
                     /locus_tag="PC700_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902013.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
     gene            complement(970..1257)
                     /locus_tag="PC700_002342"
     CDS             complement(970..1257)
                     /locus_tag="PC700_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581645.1"
                     /translation="MRLNVTKQNTIEVENSRKEDVYFVIVDNVFFERQGDVFNSITNN
                     NLNNYDVKILPTDNVFLELKITSTKDLEPVFIVVEDRQYNHGSIMFKPVLV"
     gene            complement(1277..1552)
                     /locus_tag="PC700_002343"
     CDS             complement(1277..1552)
                     /locus_tag="PC700_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581646.1"
                     /translation="MSLFVGGWSKFRAVEEEDLKVFNEAIGMLKGVDYEPLIVATQVV
                     AGTNFKFICNATSVTNPPHDYLAEIIVFKPLPCDEHNATITSINIIK"
     gene            1740..2559
                     /locus_tag="PC700_002344"
                     /pseudo
     CDS             1740..2559
                     /locus_tag="PC700_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418025.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(2818..5385)
                     /locus_tag="PC700_002345"
     CDS             complement(2818..5385)
                     /locus_tag="PC700_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HDF2581647.1"
                     /translation="MIYYVLPLEHKQATIEIVGGKGMSLSKLLTAGIPVPDGFHVTTA
                     SYQIFVETNHIQSRINKLLDGIDSNNTNQLEDVSKKIGELFHNGEMPQEVSDAIKTAY
                     AGLGNISVAVRSSATAEDLPDASFAGQQETYLNIQGEDKVIDAVKRCWASLWTARAIA
                     YRVKNNIKHEIVALAVVVQKLAFSDSSGIMFTLNPINGRRSEMIINAAWGLGEAVVSS
                     LVTPDTIVVDKDSERIISYEVANKEIMTVRTSEGTEETMVPERLRKKYALTRNQVMQL
                     IQLGKKIEKYYQMPMDVEWALEKDKLYIVQARPITVLPPEWVLPEQDVVYTKGSLAEH
                     LPNPVTPLFATLGLEIVNRASELLWIDMFGKSAKKLLPKNGAYTVINGYVYLSAKSKP
                     LLIAIKSLSPRSLRRTLMNSVARWETARKGYEDAIKQWEEKPLHTMNAHQIMDGIQTI
                     FYAACIYFTRIQLTLPAASISETLFTKLFQGAVRRAGITDTSVFLLGFDTIALQSEKE
                     LWGLSEWVKQNNSINLYLQNSPAIKIAEDFMSSVMPVGVSQEVWIEWKNRINKYFKEF
                     GRTAYEFDFAYSTPQETLTPTFESVKTFVEGKGESPFLRQTTFEKRRKHAEEEILQHI
                     GGLRKKLFFKLLHWAQETSPMRENAIYLMGMGHPLIRCMFQEISERFIRGGAISHLDD
                     IYWLTKSELEVLIEQLDKNKSLSDMNGIIPVRKAELKKYMGYVSPSKLPEKNKKTISQ
                     APQKQKDGKIVLTGIGTSTGVVTAPACVLNSPADFESFQPGSVLVAVTTTPAWTPLFS
                     SASAIVTDIGGPLSHSSIVAREYGIPAVMATHIATRTIKSGQMITVDGLAGTVTFDE"
     gene            complement(5388..5993)
                     /locus_tag="PC700_002346"
     CDS             complement(5388..5993)
                     /locus_tag="PC700_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2581648.1"
                     /translation="MPKGIVLTPEQQAERREKIVAVALSLMAENGFQKTSMREIAVLA
                     NMGKSSLYDFFKTKDEIVVYAVEKEIEKTIQQVHRIIDGEPSPQQCLRKIMLNHLRFP
                     EQYRTVLMWLNTESDYLEEEYRKRLKDARYAYQDIIKSVIDNGVTTGIFRKTDTALVT
                     RLLINSMLSISYTSRPSDSSEKMLDETMNIFLHGIMNDGGE"
     gene            complement(6367..7827)
                     /locus_tag="PC700_002347"
     CDS             complement(6367..7827)
                     /locus_tag="PC700_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HDF2581649.1"
                     /translation="MFNELKTKENVYKNLINGKWVESNSRKPIEIYSPIDNSLVGKVQ
                     SMTKHEVDEVIKNTKESIKVWAEMPVYKRANIFHKAADLLLENIDEIANILVLEIAKD
                     IKSARAEVERTADFLRYTADVGKNMEGEAISGDNFPGGTRNKMSYVSRVPLGTVLAIS
                     PFNYPVNLSMSKIAPALIGGNAVVLKPATQGAISALHVVEIMRKAGIPDGVLNTITGR
                     GSEIGDYVVTHKGINFINFTGSTEVGQHISKISGMVPLLLELGGKDAAIVLEDADLDF
                     AAKNIVSGAYSYSGQRCTAVKRILVQESVADKLVGKIKPLVEKLTIGNPMDEVVITPL
                     IDNKATDFVQGLVDDALHKGAKLITGNVRKNNLFYPTLLDNVNVDMKIAWEEPFGPVL
                     PIIRVKDINQAIEIANQSEYGLQSSVFTSDIDRAFYIADKLEVGTVQINNKTERGPDH
                     FPFLGVKASGMGTQGVKYSIEAMTRPKAVVVNVREL"
     gene            8158..8736
                     /locus_tag="PC700_002348"
     CDS             8158..8736
                     /locus_tag="PC700_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2581650.1"
                     /translation="MAKSFTEIEKDNIRKRLISECEINWSKYGYKKTNIDELCSKSGI
                     AKGSFYTFFDSKEKLFFEVLNGIQNRLISSVDEILSNMPPKEGFAKSLKMLYRLYDKN
                     PFLYSPNNPDFISLINKLPQDMFLQLESNNIQSFYDLIKRHNLKLKIEKEKAFGVCNS
                     LLSSLLVKENIEYNYIEVFDFMVDNLVEHILE"
     gene            9028..10278
                     /locus_tag="PC700_002349"
     CDS             9028..10278
                     /locus_tag="PC700_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2581651.1"
                     /translation="MVKSSNTFNKNFNLMILGQIISLFGASILKFALSLYILDITGKA
                     EIFATILAVSSIPIIVFSPIGGAIADIFNRRNLMVIFDFSSSLTVLILAVFLFNNNGS
                     ILLVGIIMTILSVISTMYQPTVQSSTPLLVDNEHLMNANGIVAGVASLTNIAGPVLGG
                     VLYGVIGINAIIVISCVSFFLSAIMEIFIQIPFIKQEQNSNIIKTIFTDIKDGIVHIC
                     KENRFILKILFLAIAINLFLSSMLIVGIPYIVKITMGASNFLYGLAEGAISFSSLVAA
                     VSIGIFSKHLKITNLHISFIICAVMFIPMAVSVYPFILNTGFPVPFLIFIVSSMLIFF
                     TTTLISIYVITVTQLKTPNELLGKVMSILFAGTAIAMPLGQVMYGKLFDLLSDRVYII
                     ILGVGFITLLIAFVSKITLRNENL"
     gene            10439..11008
                     /locus_tag="PC700_002350"
     CDS             10439..11008
                     /locus_tag="PC700_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDF2581652.1"
                     /translation="MSYMPTLEQAWEILRKYNKEEFHIRHAQIVSGVMRYYAKEYDPE
                     RVDFWEIVGLLHDLDFEMYPDEHCVKQRELMTELDLDKSIIDSTISHGYGLTGSDVKP
                     EQFMEKVLFAVDELTGLIGAVAIMRPSKSVSDLELKSVKKKFKDKSFAAGCSRDVIRS
                     GAEMLGWELDKLIQTTIEAMRSLIPEMAI"
BASE COUNT         3690 a         1955 c         1579 g         3980 t
ORIGIN      
        1 gtttccctat atgctgacta ttttagctac taactcatga ctaaattcac gagtgtgcgt
       61 agctattttt taatcaatac aattactgca catatagatg cagaattttt aaatacttca
      121 cctaatgaaa ctgctgcact aatatcatgg gctactcccc tattgtcgct aaaacctgca
      181 gtatcacata attttgcatc cattccttct attggttttc tcatatttac aatgtttggg
      241 tataaagttt gagaaacagc tcctgaacca atctctgcca cagggtctaa gcaatcaata
      301 tatgatatac cagtatcttc atcttctact tccatcatta cacatcctgc aagatagtta
      361 attgcagtac ttttgccaca tcctgtccca ccaagaatta gaattaaatt ttgattttca
      421 gccttattta ttgctacttt tccttcattt aataagagtg ttatttcttc taaaagttga
      481 ttgttatttt ctggcattta ttttccccct tggttatata ttttatttaa ataaagttta
      541 atcccttaag tagattacaa agtttttaga ctatatttaa atttgttttt tatttttaat
      601 aaatttgcat tttcactcaa taattattct tctttgttta ttaaaataac tgtgttgtat
      661 aatataattt ttaccaacac agttatttta tttaatgcgc ttattttatt gtattttatg
      721 taatacagtt gtttttttat attatatgtc cactattttc ctatggttat tacatataga
      781 tttttaatta tatttgtacc attaatttaa acattcctat actactgctt aagttttaca
      841 cctataccag cttgaatatt ttatttttat ttgcttaaat agttcaacta tactggttta
      901 ataggtttcc tttagtttaa atagttcacc tatacctact tgaatagttc acttttattt
      961 gcttaaattc tataccaata ctggcttaaa cattatacta ccatgattgt attgtctatc
     1021 ttcaactaca ataaaaacag gttctaaatc tttagtagat gttattttta attctaaaaa
     1081 aacattatca gttggtaata tttttacatc ataattattt aaattattat tcgtgatact
     1141 attaaataca tcaccttgac gttcaaaaaa aacattatca actattacaa aataaacatc
     1201 ttcttttcta ctattttcaa cctctatagt attttgttta gttacattta gtctcatctt
     1261 ccacctccta ttaatattac ttaattatat taatacttgt aattgttgca ttatgttcat
     1321 cacaaggaag aggtttaaaa acaattatct ctgctaaata atcatgtgga ggatttgtaa
     1381 ctgaagtagc attgcaaata aatttaaaat ttgttcctgc tacaacttga gtagcaacaa
     1441 taagtggttc ataatcaacc ccttttagca ttcctatagc ttcattaaat acttttaaat
     1501 cttcttcttc gacagcccta aattttgacc atccaccaac aaaaagactc atataaaaac
     1561 ctcctttata tatatattta tttatatgat agtaccaatt ttcttaatat tcaatatatt
     1621 acgtctaact ggtcaataaa aatgcataac tggataaaaa gacttataaa atagcttaac
     1681 tttacatata ataagattat tctaaaatat gagacatatc ttgctttgaa tatattctat
     1741 acaacaattt tccaggcctc attgccaagt ttaaggtaac tgactttgga ttccaacttc
     1801 ttgatgctaa taaaaaatat tttgatatgt tcaaagataa tgtaaatttt ctattagatg
     1861 atataaattt agaaggttat cttaacaatg tgtataaata tcataatgat tttaaatctg
     1921 ggagatgcgt ttgtttttct atttctccac aaactattaa tggagatatt tttcacatgc
     1981 aagttgtagc tgaatgtata gattgggaag atgataagcc tatatatctt ttaatatatt
     2041 cagatgtaac gacattggta aaacaatatg tctcattatc atttttcatc caagaatttt
     2101 atcaagaaat atgataaaat agctaaaaaa tatgatattc cttctcacct tatagaaatt
     2161 gagattacag aaacaattgt atttgaagat gtcaaagtat ttagtgaaat aataaatgaa
     2221 attcataaaa atgactttaa atgctctatt gatgattttg gaagtggcta ttcttcaatc
     2281 aatatgctta agggtattta tgttgataca ttaaaattag atagagcatt ttttcaatca
     2341 gaagatttag attctcaacg tgaaagaaat ataattgtat ctataatcga actagctaaa
     2401 aagttaaaaa tgaccactgt agctgagaga atagagacca gttttcaaaa agatttcttg
     2461 caagaaataa actgtgatat gttacaagga tacgtgttct ctagaccttt gcaaataaaa
     2521 gactttgaaa aacttatgtt tcaaaactcc tccaattaac aatataaaat tgcttattat
     2581 aagtgtatta ttgtatcaac aaataaagga attgtctcaa aattgatttt ttctaatcaa
     2641 atttttattt tgagacattc tcattttttt gcataagaaa atacccacca agctacggtt
     2701 tttcttaccg ctgttcggtg ggtattagta gtattatgaa atctgaaaac tttaagtatt
     2761 actacttccg ctaacgcaat cacaaaggtc gaaagcttca aagcgaaaat aggcttttta
     2821 ctcatcaaac gtcaccgtac ctgccaaccc gtccaccgtt atcatttgtc cgctcttaat
     2881 agttcgtgtg gcaatgtgtg tagccataac ggctgggatg ccatattcac gtgcgacaat
     2941 gcttgaatgg ctaagaggac caccaatgtc cgttacaatc gcacttgcag atgaaaataa
     3001 tggtgtccat gctggagttg tggtaactgc aaccaaaaca cttccaggtt gaaagctttc
     3061 aaaatctgct ggactattca gtacacaagc tggagccgtc acaacaccag tactggtacc
     3121 aatgcccgtc aacacaattt tcccatcttt ctgcttctgt ggtgcttgtg atatagtttt
     3181 tttgtttttc tctggcaact tggacggtga cacataaccc atgtattttt taagttcagc
     3241 ttttcgaaca ggtattatcc cgttcatatc agatagagac ttgtttttat ccaactgctc
     3301 tataagtacc tccaattccg attttgtgag ccaataaata tcatctaaat gtgaaatagc
     3361 tcctcctcgt atgaatcgtt ctgagatttc ctgaaacata caacgaatca gaggatgtcc
     3421 cattcccatc aaataaatag cattttcacg cataggagag gtttcctgtg cccaatggag
     3481 caacttaaaa aatagttttt tacgcaatcc acctatgtgt tgtaaaatct cttcttctgc
     3541 atgttttcta cgcttttcaa acgtggtctg gcgtaaaaaa ggactttccc cttttccttc
     3601 cacaaatgtt tttacggatt caaaagttgg tgtaagtgtt tcctgcggtg tggaatacgc
     3661 aaaatcaaac tcataagcgg tacgaccaaa ctctttaaag tacttattga ttcgattttt
     3721 ccattctatc catacttcct gtgaaactcc aacaggcata actgaagaca tgaaatcttc
     3781 tgctatcttt attgcaggac tgttttgcag ataaaggttg atgctgttat tttgctttac
     3841 ccattctgaa agaccccaca actccttttc cgattgcaga gcaatcgtat caaacccaag
     3901 caggaaaacg gaagtatccg ttataccagc acgtcgaact gctccttgaa ataattttgt
     3961 aaataatgtt tcactgatag aagcagcagg caatgtaagc tgaatccttg tgaaataaat
     4021 acatgcggca taaaatattg tctgaatacc atccataatt tgatgggcgt tcatcgtatg
     4081 caggggcttt tcttcccatt gtttaatagc atcctcatat ccttttcgtg ccgtttccca
     4141 gcgtgcaacg ctgttcataa gtgtcctacg taaagagcgt ggggaaaggg atttgatagc
     4201 aatcaaaagt ggctttgatt tagcagaaag ataaacatat ccattaataa ccgtatatgc
     4261 gccgtttttt ggtaataatt ttttcgcact tttaccaaac atatctatcc ataaaagctc
     4321 cgatgcacgg tttacgattt caagaccaag cgtggcgaac aaaggtgtaa caggatttgg
     4381 caagtgttct gccaaacttc cctttgtgta tacaacatcc tgttctggca atacccattc
     4441 cggtggcaga acagtaattg gacgagcttg aacaatgtac aatttatctt tttccagtgc
     4501 ccactctaca tccataggca tttgataata cttctcaatt ttctttccaa gctgtatcag
     4561 ttgcattacc tgattacgag taagagcata tttttttctc aacctttcag gaaccatggt
     4621 ttcttctgtt ccttctgaag tacgaaccgt cataatctct ttatttgcca cttcatacga
     4681 tataattcgt tcggagtcct tatccactac gattgtatcg ggtgtaacta agctagaaac
     4741 caccgcttca ccaagccccc atgcagcgtt aataatcatt tcgctacgtc taccattgat
     4801 tgggtttaac gtaaacataa taccagatga atcggaaaat gcaagctttt gtactacaac
     4861 agcaagtgcg acaatctcgt gctttatatt attcttcaca cgataagcaa tagcacgagc
     4921 tgtccataag gaagcccagc atctctttac agcgtcaata actttatctt caccttgtat
     4981 gttaagataa gtttcctgct gacctgcaaa agaagcgtca ggtaaatctt cagcagtcgc
     5041 agacgaacga acagccaccg atatgttccc taatccggca taagctgttt taattgcgtc
     5101 cgacacttcc tgtggcattt ctccattatg gaaaagctcc cctattttct tagatacatc
     5161 ttcaagttgg ttggtattat tggaatcaat accatctagc agtttattaa ttcgagactg
     5221 aatatggttc gtctcaacaa aaatctggta tgaagcagtc gtaacatgaa aaccgtctgg
     5281 cacagggatg cctgctgtca atagttttga taaagacatc ccttttccac caacaatttc
     5341 aatggttgct tgtttatgtt ccagtggcaa gacataataa atcataatca ttcacctcca
     5401 tcattcataa ttccgtgcag aaatatattc attgtttcat ccaacatttt ttccgaacta
     5461 tccgatggtc gtgacgtgta gctgattgat aacatggagt taattaacaa acgtgtcaca
     5521 agagcggtat ccgttttgcg aaagatgcct gtggtcacac cattatcaat taccgatttt
     5581 ataatatctt gatatgcata acgagcatct ttaagtctct ttcgatactc ttcttctaaa
     5641 taatcagatt ctgtatttaa ccacatcagt acagttctat actgctctgg aaatctaaga
     5701 tgattaagca tgatttttct aaggcactgt tgtggtgaag gttcaccatc aataatccta
     5761 tgaacttgtt ggatagtttt ttctatttct ttctcaactg catatacaac aatctcgtct
     5821 ttcgttttga aaaaatcgta tagacttgac tttcccatgt tggctaaaac tgcaatttct
     5881 ctcattgatg ttttttggaa tccattttca gccatgagac tcaaagcaac agcaactatt
     5941 ttttctcggc gttctgcttg ttgttcaggt gtaagcacaa ttccttttgg catagggcga
     6001 tacctccttt tagcgaccaa tggtcgtttc ttttttattt atcatacagc gaccatcggt
     6061 cacttgtcaa ggttaatttt tttcattgta tatggtactc gagataaaaa tgttcatttt
     6121 agaaattgct ctatttaagt agaaataggc ttcatcatta gaaaacaaga ctataaattt
     6181 gagatattta tcatgttcaa atttcatcaa tatcaaagtt taagatatta ataaaaaaag
     6241 aattactaag aaataacata taaagattca aaaaaggagt tgaacttaaa attcaattac
     6301 aaaaaatcaa atttaagaca actcctttat tctttatctc tactattctt tatctctatt
     6361 tatttattac aactccctaa catttaccac tactgcctta ggtctagtca tagcctcgat
     6421 tgaatactta acaccttgag ttcccatacc agatgccttt acacctaaaa atgggaagtg
     6481 gtctggacct ctttcagtct tattatttat ttgaacagta ccaacttcaa gtttatcagc
     6541 tatataaaat gccctatcaa tatcacttgt aaatactgat gattgaagcc catactctga
     6601 ttgattagca atttcaattg cttgatttat atctttaact cttataattg gaagtactgg
     6661 accaaatggt tcttcccaag caattttcat atcaacattt acattatcta acaatgttgg
     6721 atagaataaa ttattttttc taacattacc tgttattaac tttgctcctt tatgtaatgc
     6781 atcatcaacc aacccttgaa caaaatcagt tgctttatta tctataagag gagtgataac
     6841 tacttcatcc attggatttc caattgtcaa cttctctacc aatggcttta tttttccgac
     6901 taatttatcc gccacactct cttgtactaa aattctcttt actgccgtac atctttgtcc
     6961 tgaatatgaa tacgctccag atactatatt cttagctgca aaatctaagt ctgcatcttc
     7021 taatactata gccgcatctt ttccaccaag ctccaataat agtggtacca ttccagatat
     7081 tttagatata tgctgaccaa cttcagtact accagtaaag ttaataaaat taattccttt
     7141 atgcgttaca acataatccc ctatctcact acccctacct gtaatagtat taagtactcc
     7201 atctggtatc ccagcttttc tcatgatttc taccacatga agtgcgctaa tagccccttg
     7261 tgtagcaggt tttaatacaa ctgcatttcc accaataagc gctggagcaa tctttgacat
     7321 tgataaattt acaggataat taaaaggaga tatagctaaa acagtaccta atggaaccct
     7381 tgaaacataa gacatcttat ttcttgttcc accagggaaa ttatctccac taatggcttc
     7441 accctccata ttttttccca catcagctgt gtatcttaaa aaatctgctg ttctctcaac
     7501 ttctgccctt gcagatttta tgtctttggc tatttcaagt accagtatat tagctatctc
     7561 atctatattt tctaacaata aatcagcagc cttatgaaat atattggctc tcttgtatac
     7621 aggcatttca gcccatactt ttatactctc ttttgtgttt tttataacct catctacctc
     7681 atgttttgtc atagattgta ctttcccaac aagagaatta tctattggtg agtaaatttc
     7741 tattggcttt ctcgaattag attctaccca ttttccattt attaaatttt tatatacatt
     7801 ttcttttgtt ttaagttcat taaacatatt tttattcctc tctctgatat aactttgtgt
     7861 atttaaatta tagccattta ttcgttatgt aaacatttct ttttttcata aacgtttacc
     7921 tataaaaaaa attttaagtt aacgtttttc tagattcttc tattttaata acatactcgc
     7981 tccaaatcta tataggtcac actttaatat caatatacca atataccaat ataccaatat
     8041 aaatattata aacataaatc acttgacaaa tttataagta gacaatatta tatatctatg
     8101 acttaaaata tttttaaagt catagatagc atatgcagat agaaaggatt gataaaaatg
     8161 gctaaaagtt ttacagaaat agaaaaagac aatataagaa aaagactaat ttcagaatgt
     8221 gaaatcaact ggtctaaata tggatacaaa aaaactaaca ttgatgaact ttgttcaaaa
     8281 tcaggaatcg caaagggttc tttctataca ttttttgatt caaaagaaaa gttatttttt
     8341 gaagtactaa atggaatcca aaacagactt atatcatcag tagatgaaat tttaagtaac
     8401 atgcctccaa aagaaggctt tgcaaaatcc ttaaaaatgt tatatcgatt atatgataaa
     8461 aatccatttt tatattcacc aaataatcct gatttcatca gcctaataaa taaactacct
     8521 caagatatgt ttttacaact tgagtccaat aacatacaaa gtttttacga cttaattaag
     8581 agacacaatc tcaagttgaa gatagaaaaa gaaaaagcct ttggtgtttg taattctcta
     8641 ctatcaagtc tattggtaaa agaaaatatt gaatataact atattgaagt atttgatttt
     8701 atggtagata atcttgtaga acacatactt gaataacaat ttaaaataat ttttctaaat
     8761 atttctttat actaaagagg attaatatga ttgaattaat gaaaaaaata aacaatatca
     8821 tttacaaaat aaatgataaa ctaaatatac acacaaaact tatagacccc agagttaact
     8881 cattccactc tactctaagc catctataat agttcgtata tttctaataa tattagatat
     8941 taaagtaaga aaaaaattcc ttataataat tgctattata tcaattatta taaatatttt
     9001 aataatctta gataggtagg tatatatatg gtaaaatcct caaacacttt taataaaaac
     9061 ttcaacttga tgatattagg tcaaataatc tcattgtttg gtgcatcaat acttaaattt
     9121 gctttatctt tatacatact tgatataact ggtaaagcag aaatatttgc tactatacta
     9181 gcagtatctt caatcccaat tatagtattc tcccctattg gtggagctat tgcggatata
     9241 ttcaacagaa gaaacttaat ggttattttt gatttttcaa gcagtcttac agtactaatc
     9301 ttagcagtat tcttattcaa caataatggt tcaatattac ttgtaggaat tattatgacc
     9361 atattatcag ttataagcac aatgtatcaa ccaactgttc agtcaagtac acccctttta
     9421 gttgataatg agcatttaat gaatgcaaat ggtattgtag ctggagttgc atctcttaca
     9481 aatattgcag gacctgtatt aggaggtgtc ttgtatggtg tgattggaat taatgcaatt
     9541 atagttataa gttgtgtaag tttctttcta tctgcaatta tggagatatt tatacagatt
     9601 ccttttatca aacaagagca aaacagcaac attataaaaa ctatatttac tgatataaag
     9661 gatggtattg ttcatatatg taaagaaaat agatttatac taaaaatact ttttttagct
     9721 atagcaataa acttattttt aagctctatg ttaatagttg gtatccccta tatagtaaaa
     9781 atcactatgg gagctagtaa cttcttatat ggtctagcag aaggagcaat ttcttttagt
     9841 tcactggtag ctgctgttag tattggtatt ttctcaaaac atctaaaaat cactaatttg
     9901 cacatttcct ttattatatg tgcagttatg ttcataccaa tggcggtttc agtatatcca
     9961 tttatattaa acacaggatt tccagttcca tttttaatct ttatagtatc atctatgcta
    10021 atatttttta caacgactct tatctcaata tatgttataa cagtcacaca attaaaaact
    10081 ccaaatgaac tcttaggaaa agtaatgtca attctttttg caggaacagc aattgcaatg
    10141 cctcttggtc aggttatgta tggtaaactt tttgatttat tatcagatag agtgtacatc
    10201 attatactag gagttggatt tattacacta cttatagcat ttgtatcaaa gataacattg
    10261 aggaacgaaa atctttagat acaaagaatt tagatataaa gatttaattt tcactaattt
    10321 ctgaatttaa atgcaaaatt gcgtatcagt atgttgacgt tttagaaatt gattgatata
    10381 atcaatggaa aataaacttt ataaaaattc aaaatattca gctagaggag tgataaaaat
    10441 gagttacatg ccaactttag aacaagcatg ggaaatatta agaaaataca ataaggaaga
    10501 atttcatata agacatgctc aaattgtatc tggagttatg agatactatg caaaggagta
    10561 cgaccctgag agagtagact tttgggaaat agttggactt ttacatgatt tagattttga
    10621 aatgtatcct gatgaacatt gtgttaaaca aagagaatta atgacagagc ttgatttaga
    10681 taaatcaata atcgattcta caataagtca tggatatgga ttaactggtt cagatgttaa
    10741 accagagcaa tttatggaga aagtattatt tgctgtggat gaacttactg gtttaattgg
    10801 tgctgttgca ataatgcgtc cttcaaagag cgtttctgat ttagaattaa aatctgttaa
    10861 gaagaaattt aaagataaat catttgctgc tggctgttct cgagacgtta taagaagtgg
    10921 tgcagaaatg cttggatggg aacttgataa gcttatccaa acaactatag aagctatgag
    10981 aagcttgatt ccagagatgg caatatagct cctcatacat ataccataga agttacatct
    11041 ttaaagagca tttctctttt tgatgcaact tctttttata ttttgtagaa taaaaagttt
    11101 aatattttaa actcactata taatctataa taaatactat aaataatata atgtataata
    11161 gtcataaact ttctaacctt aaaagtttct aatactcaat aaac
//
LOCUS       DAMJWP010000113        11088 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.118, whole genome shotgun sequence.
ACCESSION   DAMJWP010000113 DAMJWP010000000
VERSION     DAMJWP010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11088
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.118"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(355..3462)
                     /locus_tag="PC700_002351"
     CDS             complement(355..3462)
                     /locus_tag="PC700_002351"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDF2581653.1"
                     /translation="MAKFKPLVDSSVKQAEAQVFDYWKDINILEKTLEKGKDDPSFVF
                     YEGPPTANGNPGVHHVLSRTLKDSVCRYKTMSGYQVKRKAGWDTHGLPVEIQVEKELG
                     LTSKQQIEEYGIAEFNQKCRESVFSFEKQWRIMTERMAYEVDLDNPYITLDNNYIESV
                     WWILNKFNKEGYIYEGHKILPYCPRCGTGLASHEVAQGYKEVKTNTVIAKFKKKDADE
                     YFLAWTTTPWTLPSNVSLTVNADVDYLKVKKGDEVYYVSKPLADKVLGEDYEVLEEMK
                     GKDLEGLEYEQLMPFVEVDKKAFFITVGDYVTTEDGTGIVHTAPAFGEDDYNTGKRYD
                     LPVIQPVDETGKFTSTPWEGRFVMEDGLDVEIIKWLASENKLYSKEKVVHNYPHCWRC
                     QTPLVYYAKPSWYIEMTKLKDKLIENNNGVKWFPSFVGEKRFGNWLENLNDWAISRTR
                     YWGTPLPIWRCECGHTDSVGSRAELAEKAIEDVNPETVELHRPYVDDIHLKCEKCGKP
                     MTRVTEVIDCWFDSGAMPFAQHHYPFENKENFDQLFPADYISEGIDQTRGWFYSLLAV
                     STFVLGKAPYKSVLVPDLVLDKDGKKMSKSRGNTVNPMELFDQYGADALRWYLLYVSP
                     PWTPTKFDMDGLKEIQSKFIGTMKNVYNFFTLYANTDDINPTEFFVEYKDRPELDRWI
                     LSKFNNLMKDVEENLAIFELNKTLRMIQDFINDDLSNWYIRRSRRRFWATELTEDKKA
                     VYNTTYELLHGLCRAIAPFAPYMSEEMYRNLTGEVSVHLAEYPKCNEELVDTKLEEKM
                     DLAKNLVTLGRASREVERIKVRQPLQKVLVDGKFEDTISDVVDLIKEELNVKEVIFAK
                     DLDEYMNFSLKPNFKEAGPLLGSKMNLFVGALSKLNAHETANKLEKGETLTVDLDGEA
                     FEFNKDLVLIGITAKEGFNVSVENNLFVILDTKLTEELINEGYAREFISKVQQLRKSN
                     NFEVLDNIVIEYCGDDEIAKAVDHFNEYIKSETLALEINRVDDKSLEEQNLNDHMTGI
                     KVIKK"
     misc_binding    complement(3555..3787)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(3962..4489)
                     /locus_tag="PC700_002352"
     CDS             complement(3962..4489)
                     /locus_tag="PC700_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HDF2581654.1"
                     /translation="MLTPIEIENKEFKKGLRGYRDEEVDEFLDIIKEDYESLCRENTA
                     LKEKLGLYQDQVNKYENIEETLKATLITAQSAAEDTCSAANKKAKIIVEEADLKARQI
                     IEQANNRVIEIRKEYDALVKEFKIFRNKFKSLLEDEIRSVDEIFYDVDEKCVNAFEGT
                     TVYNYSNDEAATTLE"
     gene            complement(4524..5309)
                     /locus_tag="PC700_002353"
     CDS             complement(4524..5309)
                     /locus_tag="PC700_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HDF2581655.1"
                     /translation="MDKLKLTNHIKDIDLKNKMFKVIDKANSCIKNYDVKSTEFLNPY
                     EVKNAVAILNSTNEIKYSVDGGYEQAERSTVFIYPFYMEYEDIEDTLRFLQIEGNFKF
                     KNISHKDYLGSILGLGIKREKIGDIIIHDSFCQVVVSSDICDFIIVNLEKVSRNNVIV
                     KEISRENIVNSSSKYKEVSFTVSSDRLDCVISGIYNISRQDSAKYINGEKVHVNYEKI
                     TSTSKIVKNDDLISIRGKGRAKVTQIGDITKKGKIKVQARLIV"
     gene            complement(5319..5576)
                     /locus_tag="PC700_002354"
     CDS             complement(5319..5576)
                     /locus_tag="PC700_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HDF2581656.1"
                     /translation="MGTIRIALYYLLDIIAWMIVIKSLMTWFPNGRQSKIFNILENLT
                     EPIEGPIRSIMYKYTNGPIDFSPLIAIVLLMFLRQLVLVIF"
     gene            complement(5595..6053)
                     /locus_tag="PC700_002355"
     CDS             complement(5595..6053)
                     /locus_tag="PC700_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HDF2581657.1"
                     /translation="MSNGIISKFKNWIVDEEEDYIEDEYESGMDDIVQEEEMNSGFST
                     AKANKIVNLHTTSQMKVVIVEPKVYDEAATIADHLKQRRAVIVNLEGLTNSEVRKSIF
                     NFMNGAVYVLDGSIQKVSKSIFILAPNNVDIDANMKKELESKAFFPWQNK"
     gene            complement(6093..6797)
                     /locus_tag="PC700_002356"
     CDS             complement(6093..6797)
                     /locus_tag="PC700_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HDF2581658.1"
                     /translation="MGSIKDNLSEIRNKIDKAAKKVDRDSKEITLLAVTKTVDVDLVN
                     EAMREGITSVGENKPQELARKYDIIGDKLNWHLIGTLQTNKVKYIIDKVCMIHSLDRI
                     ALCEEIQKRAEQINREIDCLVQVNISKEESKHGLDREEVVDFVKEVSEKYKNIKIKGL
                     MTMAPFIEDEDEIRGVFKGLKDLSKQIKELNIPNVGMDVLSMGMSHDYEIAIEEGATI
                     VRVGTSIFGERNYNKK"
     gene            complement(6807..7442)
                     /locus_tag="PC700_002357"
     CDS             complement(6807..7442)
                     /locus_tag="PC700_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581659.1"
                     /translation="MKKVKYTRLVILGILIYLVFNILVMIISKNVDTLVLKNEKVDAK
                     IPVKGTIEKDANDQSNYLSTYISKEDSNNFEKNQNVIIEYHEQKLDAKVYKIYENNNK
                     IMVKLKISNQIVANQDTSVEEFDIIYNQMECLKIPKTSIKTKDNKRGVYVIDEQSQSV
                     KFVILEGITYENESSVFVDYYKNDINGVTSVNLYDKIILRPNSINTNIRIK"
     gene            7823..8863
                     /locus_tag="PC700_002358"
     CDS             7823..8863
                     /locus_tag="PC700_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2581660.1"
                     /translation="MSEILLGTGLLLLVLCCFTIFSYKAPYGMKAMGALANAACATFL
                     VEAFNLSLLGDVLGMEFFRSVGAANGSLGGVAAAILVPLALGVSPVYAVLIGLSCLDF
                     SILSGFVAGYFVSFLIKFIEKKVPPGVDLVVVILLCAPLSRLIASNVDPLVNSTLLQI
                     GQVLIEATNISPIIMGIILGGIITVVGTSPLSSMALTSIIGLTGLPMAIGALGAFGSA
                     FMNSTLFAKLKLGSKKDIISVAIEPLTQSDIISANPIPIYSTNFFGGAFSGIIVALMG
                     LVNMTPGTATPVAGFAIMFAYNPVQQVLITAGLCALFNVIAGFLGSKIFSTFKVLTAG
                     EIRGTDVEEEIA"
     gene            complement(9326..10681)
                     /locus_tag="PC700_002359"
     CDS             complement(9326..10681)
                     /locus_tag="PC700_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HDF2581661.1"
                     /translation="MNVGNILETIKMIDEEQLDIRTITMGISLLDCIDPDGDKAREKI
                     YNKIMSSAKDLVKVGKDIEREFGIPIVNKRVSVTPISIIAGATDENDYVKFAQTLDKA
                     AEDLGIDFIGGFSALVQKGYTKGDKILIKSIPKALASTNKVCASVNVGSTRCGINMDA
                     VKEMGEIIKETAELTKDAKGFGCAKLVVFCNAVEDNPFMAGAFHGVGEADRIINVGVS
                     GPGVVKRALEKVKGEPFDVVSETVKKTAFKITRVGQLVAKEASSRLDVPFGIIDLSLA
                     PTPAIGDSVANILEEMGLEVVGTHGTTAALALLNDAVKKGGVMACSHVGGLSGAFIPV
                     SEDAGMIDAVIKGALSIDKLEAMTAICSVGLDMIAVPGNTTAGTLGAMIADEAAIGMI
                     NNKTTAVRIIPAPGCDVGDMVEFGGLLGRAPVMPISTNSSELFTQRGGRIPAPIHSFK
                     N"
     gene            complement(10707..10976)
                     /locus_tag="PC700_002360"
     CDS             complement(10707..10976)
                     /locus_tag="PC700_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDF2581662.1"
                     /translation="MKAILTVIGKDKPGIVAGISDELYKQNINIVDISQKILQDYFTM
                     IMIVDLEKSLNSFEKTVEDLVERGKKLSVDVKIQREEIFNSMHNL"
BASE COUNT         3362 a         2020 c         1236 g         4470 t
ORIGIN      
        1 tcacgagtgt gcgtagctat tttttaatca atacttaact ttttgttttc accatacatc
       61 aaaacgcacc cctttgcata ataaataaac caccctgcag aacacacgat aaatttataa
      121 ataaatttat cagaagtagc ttactaaaaa tattattcat cctcacttaa acgtttattc
      181 attaatattc tatcacatga cataaataat ttatatgatt tatatagatt tatttattgt
      241 aaattttgga atattaaaac attttattga atagataatt atattacata aaaaatccct
      301 atagaaatct ataaactaat ttctataggg attttggtta actaactatt ctttttattt
      361 ttttataact tttatacctg tcatatggtc atttaagttt tgttcctcta aagatttatc
      421 atctactctg tttatttcta aagctaaagt ttcagacttg atatattcat taaaatggtc
      481 aacagcctta gcaatttcat catcaccaca gtattctatt actatattat ctaatacttc
      541 aaagttatta gattttctta attgctgtac tttagatata aattctctag cataaccttc
      601 attaatcaac tcttcagtta gcttagtatc tagtataaca aaaagattgt tttcaacaga
      661 tacattaaat ccttccttag cagttatacc tattaatact aaatccttat taaattcaaa
      721 agcttcgcca tctaaatcaa ctgttaaagt ctcacctttt tcaagtttat tagcagtttc
      781 atgagcattt aatttactta atgctccaac gaataaattc atcttagaac ctaataatgg
      841 ccctgcttcc ttaaagtttg gttttaaact aaagttcata tactcatcta aatccttagc
      901 aaatattacc tctttaacat taagctcttc ttttattaaa tcaactacat cacttattgt
      961 atcttcaaac ttaccatcta ctaacacttt ttgtaatggt tgacgaactt ttattctttc
     1021 tacttctctt gaagctctac ctaaagtaac taagttttta gctaaatcca ttttttcttc
     1081 taatttagta tctactaact cttcattaca tttaggatat tcagctaaat gaacagatac
     1141 ttctcctgtt aagtttctat acatttcttc tgacatatat ggtgcaaatg gagctatagc
     1201 tctacaaaga ccatgtaaca attcataagt tgtattgtac actgcttttt tatcttctgt
     1261 taattcagtt gcccagaatc ttcttcttga acgtcttata taccagttag ataaatcatc
     1321 atttataaaa tcttgtatca tccttaaagt tttatttaac tcaaatatag ctaaattttc
     1381 ttctacatct ttcattaaat tattgaactt agataatatc catctatcaa gttctggtct
     1441 atctttatat tctacaaaaa attcagttgg atttatatca tctgtgtttg catataaagt
     1501 aaagaagtta tatacattct tcatagtacc tataaattta ctttggattt cttttaatcc
     1561 atccatatca aatttagttg gagtccatgg tggagaaaca tatagtaaat accatcttaa
     1621 tgcatctgct ccatattggt caaacaattc cattggattt actgtatttc ctcttgattt
     1681 actcattttc ttaccatctt tatcaagtac aaggtctgga actaaaacac tcttatatgg
     1741 tgcttttcct aatacaaatg ttgatactgc aagtaatgag tagaaccaac ctctagtctg
     1801 gtctatacct tctgatatat agtcagctgg gaacaattgg tcaaaatttt ccttattttc
     1861 aaatggatag tggtgttgag caaatggcat agccccacta tcaaaccaac agtctataac
     1921 ctctgttact cttgtcattg gttttccaca tttttcacat tttaagtgta tatcatctac
     1981 atatggtcta tgaagttcaa ctgtttctgg atttacatct tctatagctt tttcagctaa
     2041 ttcagctctt gaaccaactg aatctgtatg accacattca catctccata taggaagtgg
     2101 agttccccaa tatctagttc ttgatattgc ccagtcattt agattttcta accagtttcc
     2161 aaatctcttc tctcctacaa aacttgggaa ccattttaca ccattattat tttctattaa
     2221 tttatcttta agtttagtca tctctatata ccagcttggt tttgcatagt atactaatgg
     2281 agtttgacat ctccagcagt gtgggtaatt atgaactact ttttctttgc tatataattt
     2341 attttcagaa gctagccatt ttattatttc aacatctagc ccatcttcca ttacaaatct
     2401 accttcccaa ggagtagatg taaatttacc agtttcatct actggttgaa taactggtaa
     2461 atcatatctt ttacctgtgt tgtagtcatc ttcaccaaat gctggtgctg tatgaactat
     2521 accagttcca tcttcagtag ttacataatc tcctactgta ataaaaaatg cttttttgtc
     2581 aacttctaca aaaggcatta attgttcata ttctaaacct tctaggtctt tacctttcat
     2641 ttcttctaaa acttcataat cttcacctag tactttatca gctaaaggtt tagatacata
     2701 atatacctca tcaccttttt taacttttaa ataatctaca tcagcattta ctgttaatga
     2761 aacatttgat ggtaaagtcc atggagttgt tgtccatgct aagaagtatt catcagcatc
     2821 ttttttctta aatttagcta taactgtatt agtttttact tctttatatc cttgtgcaac
     2881 ctcatgacta gcaagaccag ttccacatct tgggcaataa ggaagtattt tatgcccttc
     2941 atatatatat ccttctttgt tgaatttgtt tagtatccac catacagact ctatatagtt
     3001 attatctaaa gttatatatg gattatctaa atcaacttca taagccattc tctcagtcat
     3061 tattctccat tgtttttcaa atgagaaaac tgactctcta catttttggt taaattcagc
     3121 tattccatat tcttctattt gttgcttgct agtaagacct aactctttct ctacttgtat
     3181 ttcaactggt aatccatgtg tatcccaacc agcttttctc tttacttgat aaccagacat
     3241 tgttttatat ctacaaactg aatctttaag cgttctagat aaaacatggt gtactccagg
     3301 atttccatta gctgttggag gtccttcata gaatacgaaa cttgggtcat ctttcccttt
     3361 ttctaaagtt ttttcaagta tatttatgtc tttccaataa tcaaaaactt gtgcttctgc
     3421 ttgttttaca gaactgtcta ccaatggctt aaatttcgcc atgtaaatca ccctttccta
     3481 ctaaatatta taattccaaa attttttatt ctttcacata aatttaaata ctaaaaaact
     3541 cgcccccttt aaaccaaagg gacgagttat aaatcgcgtt accaccctaa ttctatatgt
     3601 aaactaaata ctagctacat ataacactct aatagtataa cggtactaac cggcacagct
     3661 tagtagaatt taatcgttca gcctgcagct caagagtgat ttttatcttt ataaattcat
     3721 tggttctcag catacccaat tctctgtaaa attatctaaa gataaatgtc tctatcaacg
     3781 cttttaaaaa tttatctgtg tttatatgaa attttataac aaataatttg aatagtcaat
     3841 atatttttga aaaatataat aatttctatt tttcttacat ttttatccta tttttaatct
     3901 ttgttataga tttctcaaac tcacacagtt attctacata aaaaataaac aaattacaag
     3961 cttattctaa agttgtagca gcttcatcat tactataatt atacactgtt gttccttcaa
     4021 atgcatttac acatttttca tctacatcat aaaaaatctc atcaacactt cttatttcat
     4081 cttctaacag tgatttaaat ttatttctaa atatcttaaa ttctttgact aaagcatcat
     4141 actcttttct tatctctatg acacggttat tagcttgttc tataatttgt ctagctttta
     4201 aatcagcttc ttcaacaatt atcttagcct ttttattagc cgcgctacaa gtgtcttctg
     4261 ctgcactttg agcagttatt aaagtagctt ttagtgtttc ttcaatattt tcatacttgt
     4321 ttacttggtc ttgatataac cctaattttt ctttaagagc tgtattttcc ctacatagag
     4381 attcataatc ttccttgatg atatctaaaa attcatcaac ctcttcatct ctataccctc
     4441 ttaaaccttt tttaaactct ttattttcta tctcaattgg agttagcatt acatttcctc
     4501 ccagctataa tttaaattag tccttataca attaatcttg cttgaacttt tatttttcct
     4561 tttttggtaa tatctcctat ttgtgttacc ttagctcttc ctttgcctct aatagaaatc
     4621 aaatcatcat tttttactat tttagaagtt gaagttatct tttcataatt gacatggact
     4681 ttttctccat ttatgtattt tgcactatct tgtctagata tattgtaaat accacttatc
     4741 acacaatcta acctatctga agatactgta aatgatactt ctttatattt tgaagaacta
     4801 ttaacaatat tttctctgga aatttccttt acaattacat tatttcttga aaccttttct
     4861 aagtttacaa ttataaaatc gcaaatatca ctacttacaa ctacttgaca aaagctatca
     4921 tgtataatta tatctccaat tttttctctt tttataccta gacccagtat tgaccctaag
     4981 taatctttat gagatatatt tttaaacttg aaattccctt ctatttgtaa aaatctaaga
     5041 gtatcttcta tatcttcata ttccatgtaa aatggataaa taaatacagt gcttctttca
     5101 gcttgttcat aacctccatc cacactgtat tttatttcat ttgtagaatt taatattgct
     5161 acagcatttt tcacttcata tggattaaga aactccgtag acttaacatc atagtttttt
     5221 atacaactat tagccttgtc tatcacttta aacattttat ttttcaagtc tatatccttt
     5281 atgtgatttg ttagttttaa tttatccatc ctatcacctt agaatataac caatactaat
     5341 tgtcttagga acatcaataa tactattgca attaaaggag aaaaatctat aggtccattt
     5401 gtatatttat acattataga ccttattggc ccttctattg gctctgtaag attttccaaa
     5461 atattaaaaa ttttactttg tcttccattt ggaaaccaag tcatcaagct ttttataact
     5521 atcatccatg ctattatatc taaaagataa taaagtgcaa ttcttattgt tcccatatat
     5581 taaccgcctt tttactattt attttgccaa gggaagaatg ctttactttc taattctttt
     5641 ttcatatttg catctatatc cacattatta ggagctagta taaatattga tttagaaacc
     5701 ttttgtatac ttccatctaa tacataaact gccccattca taaagttaaa tatagattta
     5761 cgaacttcag aattagtcaa tccttccaaa ttcactataa ctgcccttct ttgttttaga
     5821 tggtctgcta tagttgcagc ttcatcataa accttaggtt caactattac aactttcatt
     5881 tggctagttg tatgtaaatt tacaatttta tttgcttttg cagtactaaa gccactgttc
     5941 atttcttctt cttgaactat atcgtccatg ccactttcat attcatcttc aatataatct
     6001 tcttcttcat caactatcca attcttaaat ttagatatta ttccatttga cattattaac
     6061 ttcctcctta aaatttattc aaatttgagt aattattttt tattgtagtt tctttctcca
     6121 aagattgatg ttccaactct aacaatggta gccccttcct ctatagcaat ttcataatcg
     6181 tgactcattc ccattgacaa aacatccatc cctacattag ggatgtttag ttcttttatt
     6241 tgttttgata aatctttaag acctttaaat acacctctta tctcatcttc atcttctata
     6301 aaaggtgcca ttgtcattag accctttatt tttatatttt tatatttttc tgacacttct
     6361 ttgacaaaat ctacaacttc ttctctatca agtccatgct tagactcttc tttagatata
     6421 ttcacttgta ctaagcaatc tatttctctg tttatttgtt ctgcacgttt ttgaatttct
     6481 tcgcataatg caattctatc taaagagtgt atcatacaaa ctttatcgat tatgtactta
     6541 actttattgg tttgcaaagt tcctatcagg tgccagttta atttgtcacc tataatatca
     6601 tactttctag ctaattcttg gggtttattt tcaccaacag aagtgattcc ttccctcatt
     6661 gcctcattaa ctaagtctac atcaacagtc tttgtgactg ccaaaagggt aatctctttg
     6721 ctgtctctat ctactttttt tgcagcttta tcaattttgt ttcttatctc agacaaatta
     6781 tctttaatag aacccattat tatcacctac tttatcctta tatttgtgtt tatactgtta
     6841 ggtcttaaaa ttattttgtc atatagatta acacttgtaa ctccatttat gtcattttta
     6901 taataatcca caaatacaga agattcattc tcataagtta ttccttctaa tatgacaaat
     6961 tttacacttt gtgattgttc atcaattaca tatacaccac gtttattgtc ttttgttttt
     7021 atagaagttt taggtatttt taaacattcc atctgattat atattatatc aaattcttcg
     7081 acacttgtat cctgatttgc aacaatttgg ttacttattt ttaattttac cattatttta
     7141 ttattatttt catatatttt gtaaactttt gcatctaatt tttgctcatg atattcaatt
     7201 attacatttt gatttttttc aaagttatta gaatcttctt tactgatgta tgtagataga
     7261 taattacttt ggtcattggc atctttttct atagttcctt ttacaggaat ctttgcatca
     7321 actttttcat tttttaacac caacgtatct acatttttgc taattatcat tactaatata
     7381 ttaaatacta aatatataag aataccaagt atcactaatc tggtatattt tacctttttc
     7441 aaagactctc cccctataaa attactttca ttataagtac ttcttctaaa aaatgtttat
     7501 tcctttttta taagctaata ttttacgttc taataattat tttttccata ttaaatttat
     7561 tttttatcaa tcttcctata caagactgca ttatttatat ataattgtat tataaagtat
     7621 aagtttaatt tacattttat acattaagct tactttatat gtttttatag ttatttttat
     7681 ttttagatgc aatataaaac caattaagat ttaaaataac ttttgttatg ttgacattta
     7741 tttaggtaat catttacgaa tcgggataac gttatcataa caaaagttta tgtcctaaaa
     7801 aaaatacaag gaggattttc ttatgtcaga aattttatta ggaacaggtt tacttttatt
     7861 ggttctatgt tgtttcacaa tttttagtta caaggcccca tatggtatga aggctatggg
     7921 agctctagca aacgctgcat gtgctacttt cttagtagag gcattcaacc tttctttact
     7981 tggtgatgtt ttaggtatgg aattttttag aagtgttgga gctgctaatg gtagtcttgg
     8041 tggagttgct gctgctatac ttgtgccttt agccttaggt gtatctccag tttatgcagt
     8101 tttaatagga ttatcttgtt tagatttttc tatattatct ggattcgttg ctggatactt
     8161 tgtttctttc ttaattaagt ttatagagaa aaaagtgcca cctggagtgg atttagtggt
     8221 tgtaattctt ctatgtgcac cgctatctcg tcttatagca tcaaatgtag acccattagt
     8281 taatagtaca ctactacaaa ttggacaagt tttgatagaa gctaccaaca ttagccctat
     8341 aataatggga attatactag gaggcataat aacagtagtt ggaacgtctc cacttagttc
     8401 aatggctctt acatctataa ttggtcttac tggtcttcct atggcaatag gagcattagg
     8461 tgcttttggt tcagctttta tgaactcaac actatttgca aaattgaaat taggttctaa
     8521 aaaagatatt atatctgttg ctattgaacc attaacccaa tcagacataa tttcagctaa
     8581 tccaatacct atatattcta ctaatttctt tggaggagcc ttttctggta tcattgtagc
     8641 cttgatgggt cttgtaaaca tgacaccagg tacagctaca cctgtggctg gttttgctat
     8701 aatgtttgct tataatccag ttcaacaagt tcttataaca gctggtttat gtgctttatt
     8761 caatgtaatt gcaggatttt taggttcaaa aatattcagt acctttaaag ttcttactgc
     8821 tggtgaaata cgtggtactg atgtagaaga agaaattgct taaaaaacaa taagatttat
     8881 aaatttatat ctcaagtagt agagttttat attgtaggca attttattaa ttgcaaacgt
     8941 tttatttttt atttatgttt atgattatta tgtgtgttga atatttagat aaatttttat
     9001 ttattatttt tatttagatt tttgtttagt tttttatttg gttgatatat taggaataaa
     9061 aattagtata gtttattatt agttagatag cataactttt tagatacata aaaaatttta
     9121 tattaattag atattattaa aataaaagcg atacatgaat ttttaaatgt ccatgtatcg
     9181 ctttattttt gccataatct taacaactga tttttaaagt ttattatctt aacgaagttc
     9241 attatcttat ataatattta aatgcataga cttctttaat ttaaccatct aaatatttaa
     9301 ttaaatatta ttatatgtaa tataactaat ttttaaatga atgtatagga gctggaatcc
     9361 ttcctcctct ttgagtaaat aattcagatg aatttgtact tataggcatt actggtgctc
     9421 gtcctaaaag tcctccaaat tctaccatat cacctacatc acatcctggg gctggtataa
     9481 ttctaacggc tgtagtctta ttattaatca tacctattgc tgcctcatca gctatcatag
     9541 ctccaagcgt acctgctgta gtatttcctg gcacagctat catatcaaga cctacagaac
     9601 aaatagcagt cattgcttca agcttatcta tacttaaagc acctttaata acagcatcaa
     9661 tcatacctgc atcttcagat actggtataa acgctccact aagtcctcca acatgtgaac
     9721 atgccattac tccacctttt ttaacagcat catttaaaag tgctaatgct gctgtagtcc
     9781 catgagtacc aacaacttca agacccattt cctctaatat atttgctact gaatcaccaa
     9841 tagcaggtgt aggagctagt gataagtcta ttatcccaaa tggaacatcc aatctactag
     9901 aagcttcctt tgccacaagt tgtccaactc ttgtaatttt aaatgcagtc tttttaactg
     9961 tttctgaaac aacatcaaaa ggctctccct ttactttctc taaagctctc ttaacaacac
    10021 cagggccact tacaccaaca tttataattc tatcagcctc accaacacca tggaaagctc
    10081 ctgccataaa tggattatct tctactgcat tacaaaatac tactaatttt gcacaaccaa
    10141 aaccttttgc atcttttgtt aattctgcag tttcttttat gatttctccc atttctttca
    10201 ctgcatccat atttatacca catcttgtag aacctacatt tactgaagca catactttat
    10261 tagtacttgc tagagctttt ggaattgact taattagtat cttatcacct tttgtatatc
    10321 ccttttgaac taatgctgag aatccaccaa taaaatctat acctaaatcc tctgcagcct
    10381 tatccaatgt ctgtgcaaac tttacatagt cattttcatc tgttgcacct gcaataatgg
    10441 aaattggagt tactgatacc cttttattta caattgggat accaaattca cgctctatat
    10501 cttttccaac cttcaccaaa tctttagcag aactcataat cttattgtat attttttctc
    10561 tagctttatc tccatcaggg tctatacaat ctaataaaga aatccccata gttatagttc
    10621 ttatatccaa ctgttcttca tctatcatct ttattgtttc aagtatattt cctacattca
    10681 ctattatcac ctccccttac tgtctactac agattatgca ttgaattaaa tatttcttct
    10741 cgctgaattt ttacatccac tgataatttc ttacctcttt caaccaagtc ctcaaccgtt
    10801 ttctcaaaag aatttaatga tttctctaaa tcaactatca taatcatagt aaaataatcc
    10861 tgtaatattt tttgtgatat atctactatg ttgatatttt gcttgtataa ttcatcacta
    10921 attcctgcca ctatacctgg tttatccttt ccaattactg ttaaaattgc tttcataata
    10981 atatcctccc ttattttttt ataaatctta tatttttaca aattgaacta tgtattaaca
    11041 tatatttcat ttatttgtta gtaaaccact ttttatgtat atatttaa
//
LOCUS       DAMJWP010000114        10945 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.119, whole genome shotgun sequence.
ACCESSION   DAMJWP010000114 DAMJWP010000000
VERSION     DAMJWP010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10945
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.119"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            197..592
                     /locus_tag="PC700_002361"
     CDS             197..592
                     /locus_tag="PC700_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581663.1"
                     /translation="MNILKMKKTGIFLGGVLFGTVGVKILSSDCVKKTCTRATAGALK
                     VKDSVLETTTKIQENVEDILAEAKEINEQEDLSTSKVNVVQDTLKCEQEVLKTETIKT
                     VEDTNSNNIKCEEIKDNVIEEKNNTCTTE"
     gene            635..2734
                     /locus_tag="PC700_002362"
     CDS             635..2734
                     /locus_tag="PC700_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDF2581664.1"
                     /translation="MNFYIKHEIRGRIRFDLQLGKLNNKQSDTLLYYLTSINGITKAK
                     VYDRTGDAVIYYTGEREYIIREITKFSFNNSKIENLVPEHTSRELNNYYRDKLANKLL
                     VKSISKIFFPKSLRFFLIGLHSIKYFKKGLSSLLNKKIEISVLDATAIGISIFRKDIN
                     TAGSVMFLLGIGELLEEWTRKKSIDDLAQSMSLNIEKVWLKKDATEILVPISEIKEGD
                     LVSVTMGNMIPLDGVIVSGEAMVNQASLTGESLAVNKKEGSYIYAGTGIEQGNIVMCV
                     KEKAGTTRFQKIVTMIEESEKLKSSVESKFEHLADTLVPYSFLGSILTYAITRNPIKS
                     LSILMVDFSCALKLSIPISVLSAMRECNNHSIAVKGGKFLEGVACADTIVFDKTGTLT
                     KAQPTVSDIITFQNYNKKDMLRLAACLEEHFPHSIANAVVYEAEKQGLSHKEMHTEVE
                     YIIAHGISTKVDDKKVVIGSKHFVFEDEKCTIPDGEEDKYNNLSDEYSHLFMAISGKL
                     SAVICINDPLRKEAKYVISNLREYGIKKIVMMTGDSEKTAKSIASKVGVDEYYSEVLP
                     EDKANFVKSEKLKGRKVIMIGDGINDSPAISESDVGIAMSEGAEIAREISDITISADN
                     LNNLIVLKQISNKLMKRVDLSSKFIIGFNLGLILLGVSGFVRPSTSAFLHNASTVGIS
                     LNSMTNLLENTNTYNYY"
     gene            complement(2959..8169)
                     /locus_tag="PC700_002363"
     CDS             complement(2959..8169)
                     /locus_tag="PC700_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HDF2581665.1"
                     /translation="MANKLYSEIVNLLEEGRDELRKYDLKEKSILLFLGASGVGKSTC
                     INYLKGCVMEEKTDEETGQIYITAKDSAVEIGNGVYSKTLCPEVVDIANRNFSLCDCP
                     GFFDNRGAEYMIAGAMLVRETISTSSKVKGMVVILDYQSLLDSRHTLLIETGKNLKDM
                     LGDYAKYKENFFFLINKIPKTLIGNVTEEKIRNILEKAKEDLDSQEKDYDGCKELVYL
                     LNVALSDGVSIKLCNPLDRQPEKLLDEMKNLPETFEKTDLGLMLTASCKIEIQKAVDV
                     TYKDLEKLLVPYIESVKKGMEDSISILSSINSLKDKKTQLDKMYTDLCSRTNNMKELM
                     EFVQELSYGDGVQKVNILEETRLLLELNSYCSETHIVGLSELLKSLLKNTLEDTLKDI
                     KYWIAEEEYREEEAKKLEDAEYRIEKIQSCTYIKSYEKENVLSIYIPIPKVNLSDLTK
                     IISGYQLNILSSIYMYGDCELKIDCNMEKEVFSGKNIVLLTEKLCIPEGIYSINVSGK
                     DAEKLVGEGSIYDGMNGRNGNNAGTSGDILLSFKEIEKRGELKLYVDGGRGGNGQDGK
                     RGDTGITGEAGENAKIGSKKESKEFAKVKGTKYYVVKAEKGKQGGTGGTGGNGGNAGL
                     GSKSGQVYLLGKASLLKGAISKKDGNHGLAGKGGSGGAGGAGGLGGIRGIYEETIRYI
                     GTSAPIDDSDRNYYYFDRYVFNHASNENQVADDGEKLNALYEKHKDWTISKREDSGLV
                     GAEGKDGRSGDALKEKELLKQEYSLSIDTFFYEFFERFGFSGIKGYRNLRLLCAVGIL
                     EADILELSHIYEIFHAVEKIEMQLNREILQKYYSFEKKNKAVLSLDDMNIKKEFYKEA
                     KEIYEYLINKLMQSDRIESFIGEKKNFSEWSMILFALEQKTAFYVQMIEESEIGGNRV
                     TDIRSLTDYLDKTLRELEQGKKDELKEGYFNTYKESILTQIESGNQQIEVLKDELENK
                     MLSVNAKMNLLIQEIIKMKEEEQEDIEELKKKQAKLEEQMKTQLILSGIQSALSLLTA
                     FSGVGQACLGAFDSAKSLKENLSAKEEEYQWTNRMVDFSDFKLDSDGMRFVLGEQLSE
                     DRKNALDKMTYNLAGVDEERRTKFDQIKEIIVKSIDDDNYAEKADDIQRAKEGLSESE
                     YKEIYTKIQKKEIGMLEANYNNKQQKDKLAALEKSKQAIERQQKTSKIKNALSIGSSA
                     CSLINTAISGVNNCLNVKSSFESKIEVVKEAIKDKENNIKKLDELNTQMVEFKDKTLK
                     ESVIPFVEKFDSTVKNQDIFENEISKLKMKEVLTELSTKFGSLKMENSEEISIIFKKV
                     QDMMETQIIITDKIDKKKDNLAMGELIYQMSGSGNQMNEYQKKLIHQIELNQVRYLCQ
                     VEAAAFQLWTFPFGSNIVNILNRCHVGITEDENEVINQAKEQNRQIREFLDQDAYQWQ
                     KTDNHILKVALTGSLDGKSRVGFENTYSLYDCNEEEVKKLLTGDEISFEVPLNKNFDA
                     IKYVTMYANIPNLRRNILEDSLKVKVHIHLYDSGYFSLYDDKKDVYYQYVFPQNYIDI
                     THGLDVKYNDSDSINSIWSSVEDKGIEKFKNAGLNSGRSPYSMAKISLKAHFNKKLSY
                     DSLTNEQKKKLESYFDKPSKYAWSESIDILKSLGYIDLKGILSKSWYEIKKDIDSDGE
                     IMKHFSILQVDLLERIIYEQTSILKENIPNLTIELVGCGIYVESEFNYLKQNDLKCYE
                     ERLVENTIIC"
     gene            complement(8184..10307)
                     /locus_tag="PC700_002364"
     CDS             complement(8184..10307)
                     /locus_tag="PC700_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2581666.1"
                     /translation="MQNSVKQEPCDFSHGRFRPEADILDSRLTKVLDLFQQLNMCLHK
                     DNFRGSITFFISKSFLGRTEKQIYNVIKRKYEQLCENLDSNIEEKAWLFEELLKDNGK
                     NIHLCKPLESDEREGLLKQVLELDEVKDKTSAFQYPISADARLVLDTLIKEIQNNIVS
                     SLEDYVKAYTEDFISIIGSITMIQEIAKYDEVVKVFKNWLESTTTLTIERFINVLAKQ
                     SGLFLQDSCMELQNKIKGNLDIIDALSKYTEHGTGEILLPSVHMEFIIEKASKKIMEQ
                     NFIIIRKNLEKVLVSYEVQKQVFTLPNREKLMEVANKKDYVYSVSDFNSILTNVTVTQ
                     NEELEDSITRLSAYGEEGNEIIREFIGKYILNPFIVKTININDKKGIEIRALIPNLVI
                     SSVLEYFKNRKVDDNVSTFMFFAKDTIYMDDNLGMDFASEKNIILACNNLDVGSNVKI
                     NVSGKSGELVDSSMYGKNGEDGRDAGNIYLYIKEEIRNYRLTLIANGGNGSKGIDGKN
                     GETGDSGSNGADAGYTSSFHGQMFKGYFAKGTSGKKGDKGGAGGNAGRGGNAGNKGNI
                     YFISDSEEQKNRIINHCDITVKDGEAGPNGRPGSGGYGGSGGYHGYNTLSFSPSGIDK
                     TVYYTGYFTSWDYKLDSIIKYHIEFSGGSWNEGKTGPAREYAEYGDRGDDGGFNAPNT
                     KLSTAVSKVEENLIKEKYQGYCSEI"
     gene            complement(10372..>10945)
                     /locus_tag="PC700_002365"
     CDS             complement(10372..>10945)
                     /locus_tag="PC700_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2581667.1"
                     /translation="GLDLGIKEFCISSCGDFIENPKYLKKSLNKLAKLQRELSRKTIG
                     SLNRNKARLKVARLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNMIKNRKLSR
                     LISDVSWSEFIRQLKYKANWHGRQIVKVGKFFASSQICNKCGYKNEEVKDLSIREWIC
                     PSCNETHDRDINASINILKEGLRLITIQNK"
BASE COUNT         3493 a         2045 c         1285 g         4122 t
ORIGIN      
        1 tgcatatata agctatataa aaattatttc aactatataa actacacatg taaaattcat
       61 gtaaatttac taatataaat caaataaaat tactaaactc tctaaactaa ttttgtaaaa
      121 catactctat ttttaacatt tgttacctta tacataagta taaacaaaat ctaactaaat
      181 gaaaggataa tgtaaaatga atattttaaa aatgaaaaaa acaggaatat ttttaggagg
      241 agtacttttt ggtactgtag gtgttaagat actttcaagt gactgtgtaa aaaaaacttg
      301 cactcgtgca acagcaggag cattgaaagt taaagattct gttcttgaaa caactacgaa
      361 aattcaagaa aatgtagaag atattttagc tgaagcaaaa gaaatcaacg agcaagaaga
      421 cttatctaca tctaaggtaa acgttgtaca agatactctt aaatgcgaac aagaagttct
      481 taaaactgaa actataaaaa ctgtagaaga tactaattct aataacatta aatgtgaaga
      541 aatcaaagat aacgtaattg aagaaaaaaa taatacttgt acaacagaat aaatttaatg
      601 attattatga ttaataatat caggaggaag taaattgaat ttttatataa aacacgaaat
      661 aagaggtcgt atacgctttg acttacaact tggcaaatta aacaacaagc agtccgatac
      721 tctcttatat tatttaactt ctataaatgg tattaccaaa gcaaaagttt acgatagaac
      781 tggtgatgca gtcatttatt acactggtga aagagaatac ataataagag aaatcactaa
      841 attcagtttt aacaatagca aaatagaaaa cctagttcct gaacatacca gcagggaatt
      901 aaacaattat tatagagaca aattagcaaa taaactgcta gtcaaaagta tctccaaaat
      961 attttttcca aagtccttaa gatttttttt aattggactt cattctataa aatattttaa
     1021 aaaaggtctc tcttccctac taaataaaaa aatagaaatt tctgttttag atgccactgc
     1081 tataggtatt tctattttta gaaaagatat caatacggct ggttcagtta tgtttcttct
     1141 tggtatcggt gagttattag aagaatggac tagaaaaaaa tctatagacg acttggctca
     1201 aagtatgtca ctaaacattg aaaaagtatg gttaaaaaaa gatgccactg aaatactagt
     1261 tccaatttca gagataaagg aaggagattt agtcagtgta actatgggaa atatgattcc
     1321 attggatgga gttatagtat caggagaagc aatggtcaat caggcatcac ttacaggtga
     1381 atcccttgca gttaacaaaa aagaaggctc ttacatatat gctggaactg gaatagagca
     1441 aggcaatatt gttatgtgcg tcaaggaaaa agctggtact actagattcc agaaaatagt
     1501 aactatgatt gaagaatctg aaaaactaaa atcttctgta gagagtaaat ttgaacatct
     1561 agcagataca ttagttccat acagcttttt aggaagtata ttaacctatg caattactag
     1621 aaaccctata aaatcactct ctatattgat ggtagatttc tcttgtgcat taaaactctc
     1681 tatccctatt tcagtactat cagccatgag agaatgtaac aatcacagca ttgctgtaaa
     1741 aggtggaaaa ttccttgaag gtgttgcatg tgcagatact attgtatttg ataagacagg
     1801 cacattaaca aaagctcaac caactgtttc agatataatt acattccaaa attataataa
     1861 gaaggatatg ttaagacttg cagcttgctt agaagaacac ttcccacact ccatagcaaa
     1921 tgcagtagtt tatgaagctg aaaaacaagg gcttagtcac aaagaaatgc atacagaggt
     1981 agaatatatt attgctcatg gcatttcaac aaaggtcgat gataaaaagg ttgtaatagg
     2041 tagcaaacat tttgtatttg aagatgagaa gtgtactatc ccagacggtg aggaggataa
     2101 gtacaacaat ctatcagatg agtattctca cctatttatg gcaatatcgg gtaaattatc
     2161 agcagttatt tgcataaatg accctttacg aaaagaagca aaatatgtaa tttcaaattt
     2221 aagagaatat ggaataaaaa agattgtgat gatgactgga gatagcgaaa aaacagctaa
     2281 atctatagca agtaaagttg gtgttgatga atattattca gaagtcttac cagaagacaa
     2341 agccaatttt gttaaaagtg aaaaactaaa aggtcgcaaa gtaattatga ttggtgatgg
     2401 aatcaatgat tctcctgcta tttcagaatc agatgtcgga attgcaatga gtgaaggtgc
     2461 tgaaatagca agagagattt cagatattac catatcagca gataatttga ataatttaat
     2521 cgttttaaag cagattagta ataagcttat gaaaagagtt gatttaagtt ctaagtttat
     2581 tatcggcttt aatttaggtc tgattttact tggcgtaagt ggatttgtta gaccaagtac
     2641 atctgccttt ttacacaacg catctactgt tggaataagt ttaaacagta tgacaaattt
     2701 attggaaaac accaatactt ataactacta ttaatcccca ttatataaaa catatgtata
     2761 taaaaaattt ataattcatc ttttaaaaag aaactgtctt aaaaggagct tttttctaat
     2821 caaaattata taaaacatat tttacaaatt ttaaactcaa cttatttcta ttttttacaa
     2881 aaaggagatg attcaaattt tcattttgaa acatcccctt ttgtaaattt ctttaaaact
     2941 tatatattaa ttatattttc aacaaataat tgtattttca accaatcttt cttcatagca
     3001 ttttaaatca ttttgcttta gataattaaa ttcagactct acataaatac cacagcctac
     3061 caattcaatt gtcaaattag gtatattttc ctttagtata ctagtctgct cataaataat
     3121 tctttccaat aaatcaactt gtaatataga aaaatgcttc ataatctccc catcactatc
     3181 tatatctttc ttgatttcat accaactctt tgacaaaatt cccttcaaat caatataccc
     3241 tagactcttc aatatatcta tactctcgct ccatgcatac ttactaggtt tatcaaagta
     3301 tgattctagt ttcttcttct gctcatttgt aagtgaatca taagaaagct tcttattaaa
     3361 atgtgccttc aaagaaatct tagccataga atatggactt ctaccagagt ttaatcctgc
     3421 atttttgaac ttttcaattc ctttatcttc tactgaagac catattgagt ttattgaatc
     3481 tgaatcattg tatttaacat caagaccatg tgtaatatct atatagtttt gtggaaatac
     3541 atattgataa tagacatctt ttttatcatc atatagggag aagtatccac tatcatataa
     3601 atgaatatgc acctttactt ttaaactatc ctctaaaata tttcgtctaa ggttcgggat
     3661 atttgcatac atggttacat attttatggc atcaaagttt ttatttaatg gaacttcaaa
     3721 actaatttca tctcctgtca aaagcttttt cacttcttct tcattacaat catatagaga
     3781 atatgtattt tcaaatccca cacgtgattt cccatctaaa ctgcctgtta atgctacctt
     3841 caaaatatgg ttatccgttt tttgccattg gtaagcatct tggtcaagaa actctctaat
     3901 ctgtctattt tgctctttcg cctgatttat aacctcgttt tcatcttctg taatacctac
     3961 atggcatcta tttaagatat ttactatatt gcttccaaat ggaaacgtcc atagttgaaa
     4021 agctgctgcc tcaacctgac acaaatatct aacctgattt aattcaattt gatggataag
     4081 ttttttctga tattcattca tctgattacc actacctgac atttgataaa tcaattctcc
     4141 cattgcaaga ttatcttttt tcttatcaat cttatcagta atgataattt gagtttccat
     4201 catatcctgt acttttttaa atattataga aatctcctct gaattttcca tttttaagct
     4261 tccaaacttt gtgcttaatt cagttaaaac ttccttcatc tttaatttac ttatctcatt
     4321 ttcaaaaata tcttgatttt taactgtact atcgaatttt tctacaaatg gtataacaga
     4381 ttctttcaaa gttttatctt taaattccac catttgagta ttcaattcat ctaatttctt
     4441 aatattattt tctttatctt taattgcctc tttaactacc tctattttac tctcaaaact
     4501 agattttaca ttaagacaat tattaacacc actaatcgca gtatttataa gagaacaagc
     4561 agaacttcca atactcaatg catttttaat tttagatgtc ttttgctgtc tttcaatagc
     4621 ttgtttactt ttctctagtg cagctaactt atctttttgc tgtttattat tgtaatttgc
     4681 ctccaacata cctatttctt ttttctggat ttttgtgtaa atttccttat actcgctttc
     4741 acttaacccc tcttttgctc tttgaatatc gtcagctttt tctgcatagt tatcatcatc
     4801 tatagactta actataatct ccttaatttg gtcaaattta gttcttcttt cttcatcaac
     4861 acctgccaaa ttataagtca ttttatctaa agcatttttt ctatcttcac ttagctgttc
     4921 tccaagtaca aatctcatac catcagaatc caatttaaaa tctgaaaaat caaccatcct
     4981 gttagtccat tggtattcct cttctttagc tgaaagattt tccttcaaag attttgcact
     5041 gtcaaatgcc cctagacaag cttgaccaac accactaaat gcagtcagta aactcaatgc
     5101 gctttgtatt ccagaaagaa ttaactgtgt tttcatttgt tcctcaagtt ttgcctgttt
     5161 cttttttagt tcctctatgt cttcttgctc ctcttctttc atctttataa tttcttgaat
     5221 caacaggttc atctttgcat taacagaaag cattttattc tcaagttcat cttttaaaac
     5281 ttctatttgt tgatttccag attcaatttg agttaggata ctttccttat aagtattgaa
     5341 atatccttct tttaactcat ctttctttcc ttgttccaat tctcttaagg tcttgtccag
     5401 ataatctgtc aaagaacgaa tatctgtaac acgatttcct ccaatttcac tttcttcaat
     5461 catctgcaca tagaatgctg ttttttgttc aagtgcaaat aaaatcatac tccattcgct
     5521 aaaattcttc ttttctccta taaaagattc tattctatca gactgcatca atttatttat
     5581 aaggtattca tagatttcct ttgcctcctt ataaaattct ttctttatat tcatatcatc
     5641 cagagaaagc actgctttat ttttcttctc aaaactatag tacttctgaa gtatttctct
     5701 atttaattgc atctctattt tctcaactgc atgaaaaatt tcataaatat gtgaaagttc
     5761 taaaatatca gcttctaaaa ttcccactgc acacaacagt ctaagatttc tataaccttt
     5821 aattccagaa aaaccaaatc tctcgaaaaa ttcatagaag aaggtatcta ttgacaaaga
     5881 atactcctgc tttaacaatt ccttttcttt taatgcatct ccagagcgac cgtcttttcc
     5941 ctctgctccg actaaacctg aatcttcacg tttgctaatt gtccagtcct tatgtttttc
     6001 ataaagagca tttaatttct ctccatcatc tgctacttgg ttctcatttg aagcatgatt
     6061 aaacacatat ctatcaaaat aatagtaatt tctatcactg tcatctattg gagcagaggt
     6121 acctatatat cttattgttt cttcatatat acctctaatt ccaccaagac ctcctgcacc
     6181 accagctcct ccagagcctc cttttcctgc tagaccatga tttccatctt ttttacttat
     6241 agcacctttt aataaagaag cttttccaag gagataaacc tgtccagact tgctcccaag
     6301 acctgcattt ccaccattac ctcctgtgcc tccagttcca ccttgttttc ctttttctgc
     6361 ttttaccaca tagtattttg tacctttaac cttagcaaat tcttttgact cttttttaga
     6421 acctatttta gcattttccc cagcttctcc agttatccct gtatctcctc tttttccatc
     6481 ttgtccattt ccaccacgtc caccatctac gtaaagttta agttctcctc ttttttctat
     6541 ttctttaaaa gataataaaa tatcacctga agtacctgca ttattgccat ttcttccatt
     6601 cattccgtcg taaatagaac cttctccaac caatttttct gcatccttac cagatacgtt
     6661 gattgaataa atcccttctg gtatacataa tttttctgta agtagtacaa tattcttacc
     6721 tgagaagact tctttctcca tattacagtc aattttcaac tcacaatctc catacatata
     6781 tatagatgac aaaatattaa gttgataacc agatattatt ttagtcaaat ccgataaatt
     6841 cactttaggt ataggaatat atatagaaag tacattttct ttctcatatg attttatata
     6901 tgtacaactt tgaatcttct ctatcctata ctctgcatct tcaagctttt tggcttcttc
     6961 ttccctatat tcttcttctg ctatccaata tttgatatct tttaatgtat cttctaaagt
     7021 attttttagc aatgatttta acaactcact tagaccaaca atatgagtct cactacagta
     7081 gctatttaac tctaaaagaa gacgtgtttc ttctaatata ttcactttct gtacgccatc
     7141 accatatgaa agttcttgta caaactccat aagctctttc atattattag tcctactaca
     7201 taaatctgta tacatcttat ctaattgagt ctttttatcc tttagactat ttatagaaga
     7261 tagaatggaa atagaatctt ccataccttt tttgacactt tctatataag gaactaaaag
     7321 tttttctagg tctttatagg ttacatcaac ggctttttgt atttctattt tacaagaagc
     7381 agtaagcatc aatcccaaat ctgttttttc aaatgtttca ggtagatttt tcatttcatc
     7441 caacaacttt tctggctgtc tatctagtgg attacataat ttaatagata caccatctga
     7501 taatgctaca tttaataagt acactaattc cttacaaccg tcgtaatctt tctcttgact
     7561 atctaggtct tcttttgctt tttccaatat attccttatt ttttcttctg ttacattacc
     7621 gattagtgtt ttaggtattt tattgataag aaagaagaaa ttttctttat actttgcata
     7681 atcaccaagc atatctttca aatttttgcc tgtttctatc aataatgtat gtctagagtc
     7741 cagtaaggat tgatagtcta aaataactac catccctttc acctttgaag atgtagaaat
     7801 agtttccctt acaagcatag caccagcaat catatactct gctccacggt tatcaaaaaa
     7861 acctgggcaa tcacataatg aaaaatttct atttgcaata tctacaactt ctggacataa
     7921 agtcttagaa tatacaccat ttccaatttc gacagctgaa tcttttgcag ttatatatat
     7981 ttgtccagtc tcttcatccg ttttttcttc catcacgcaa ccttttaaat aatttataca
     8041 agtactcttt ccaacgcctg aagcacctaa gaataacaaa attgactttt cttttaagtc
     8101 atattttcta agctcgtctc ttccttcttc caaaaggttt acgatttcac tataaagttt
     8161 atttgccata catccatccc tttctatatt tcggaacaat agccttgata tttttcttta
     8221 ataagattct cttctacctt tgaaacagct gttgataact tagtatttgg agcattaaat
     8281 cctccgtcgt ctcctctatc tccatattca gcgtattctc ttgctggtcc tgttttgcct
     8341 tcattccaac ttccgccact aaattctata tgatatttaa ttatactatc taacttataa
     8401 tcccaacttg taaaatatcc tgtatagtaa acagtcttat ctataccact tggagaaaat
     8461 gaaagtgtat tatatccgtg atagccacca cttcctccat atccacctga acctggtcta
     8521 ccatttggac ctgcttctcc atctttcact gttatatcac aatgattaat tattctattt
     8581 ttttgttcct ctgagtctga aatgaagtaa atattacctt tattaccagc atttccacct
     8641 cttccggcat ttccaccagc tccacctttg tcaccttttt taccacttgt accttttgca
     8701 aaataccctt taaacatctg tccatggaaa ctacttgtat atcctgcatc agctccatta
     8761 cttccagaat ctcctgtttc accattttta ccatctattc ctttagaacc atttcctcca
     8821 tttgcaatca gtgttaaacg ataatttcta atttcttctt taatatataa gtaaatatta
     8881 cctgcatctc taccatcttc accatttttc ccatacatag aactatctac aagttctccg
     8941 ctctttccac ttacattaat ttttacatta ctacctacat caagattgtt acaagcaaga
     9001 ataatatttt tctctgatgc aaagtccatt ccaaggttat catccatata aatagtatct
     9061 ttggcaaaga acataaatgt agaaacattg tcatctactt ttctattttt gaaatattcc
     9121 aatacactac taattactaa gtttggaatt aatgctctta tttcaatacc tttcttatca
     9181 ttaatattaa ttgtttttac tataaatgga tttaaaatgt atttaccaat aaattctcta
     9241 attatctcat taccttcttc tccatatgca cttaatcttg ttatactgtc ttctaactct
     9301 tcattttgtg taactgtaac atttgtcaaa attgaattaa aatcacttac actatataca
     9361 taatcctttt tatttgcaac ttccattaat ttttctctat ttggtaatgt aaatacctgt
     9421 ttttgtactt cataagaaac taaaactttt tctaaattct ttcttattat aatgaagttt
     9481 tgttccatta tcttcttact tgccttttct ataataaatt ccatatgtac agaaggtaat
     9541 aaaatttccc cagttccatg ttctgtatat ttacttaaag catcaattat atctaagttt
     9601 ccttttatct tattttgtaa ttccatgcaa ctatcttgta agaataagcc actttgtttg
     9661 gctaatacgt tgataaatct ctctatagta agagttgttg tactttccaa ccagttttta
     9721 aatactttta caacctcatc atattttgct atttcttgaa tcattgttat acttccaata
     9781 atactgataa aatcttctgt atacgcctta acataatctt ctaggctaga tacgatattg
     9841 ttttgaattt cttttatcaa cgtatctaaa actaatcttg catctgcact aattggatat
     9901 tggaatgctg atgtcttatc tttaacttca tctaattcta gtacttgctt taataagcct
     9961 tccctctcgt ctgattcaag tggcttacaa agatgaatat ttttaccatt atcttttagc
    10021 aattcttcaa ataaccaagc tttttcctct atattactat ctaaattttc acataattgt
    10081 tcatattttc tttttataac attatatatt tgtttttctg ttcttcctaa gaaacttttt
    10141 gaaatgaaaa aagttatact gcccctaaaa ttgtctttat gaagacacat atttaactgt
    10201 tgaaacaggt ctagtacttt tgtaagtcta gaatctaata tatctgcttc tggtctgaac
    10261 ctcccatgac taaagtcaca gggttcctgc ttcacagaat tttgcatact aaaatatgtc
    10321 ttactaattc tccacaggcc atccccgtag ttcctacggt tcttacataa gttatttatt
    10381 ttgaattgtt attagtctta atccttcttt taatatattt atacttgcat ttatatctct
    10441 atcgtgagtt tcattacagc taggacaaat ccattctctt atacttaagt ccttcacttc
    10501 ctcgttttta tacccacatt tattgcatat ttgtgaactt gcaaagaatt tacctacctt
    10561 tacaatttgt ctaccatgcc aattagcctt gtattttaat tgacgaataa attctgacca
    10621 tgatacatca gaaattaagc gagatagttt acgattttta atcatattct ttacttgtaa
    10681 atcttctatg caaataatat cattctcttt tattaattta gtagatagtt tttgtagaaa
    10741 atcatttctt tgattggcta tatgttcttg aagtcttgca acttttaatc ttgctttatt
    10801 tctatttaaa ctaccgattg tttttctcga taattctctt tgtaatttag caagtttgtt
    10861 taatgacttc ttaagatact taggattttc tataaagtct ccacaacttg agatacaaaa
    10921 ttctttaatt cctaaatcta aacct
//
LOCUS       DAMJWP010000115        10762 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.120, whole genome shotgun sequence.
ACCESSION   DAMJWP010000115 DAMJWP010000000
VERSION     DAMJWP010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10762
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.120"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            64..912
                     /locus_tag="PC700_002366"
     CDS             64..912
                     /locus_tag="PC700_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDF2581668.1"
                     /translation="MNNQKNLNIPITISSYTLGTEVSFRDRVRIAKEAGFDGIGLRAE
                     NYIDAKNAGVTDEEMIAILNEYDMKVTEVEYITQWGTEEDRTEAQREKEQNVYHMAHL
                     FNVKHINCGLLEMIPEEQIITALGELCDRAGELIIGLEFMPYSGVPDLATAWRVVKGC
                     NRENAMLILDTWHWARAKQTAEDLAPVPADKIVSIQVCDVLETPYEKLRDESLHDRLA
                     PGEGYGNTEEFVKIIKEHNISPRVIGAEVISDSLISKGLSTAAETVFNATKKVLDKAW
                     SEVSPK"
     gene            991..2913
                     /locus_tag="PC700_002367"
     CDS             991..2913
                     /locus_tag="PC700_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF2581669.1"
                     /translation="MKISSMFTPIRIGSMTVPNRFVVPPMGNNFANTDGTLSETSKAY
                     YLERALGGFGLITIESSVVDKKAKGGPRKPCLYDDSTIDSFKNVIDACHDAGSKVSIQ
                     LQHAGSEGNEKVAGHPLKAASAIPASNGRNTPLAITTEEIYELIESYGDAALRAQKAG
                     ADAVEVHCAHGYLVSSFISQRTNKRVDEFGGCFENRMRLPRLIIENIRKKVGNSLAIL
                     CRINSTDDVPGGIDIHDSSVIAAYLEGCGIDGLHVSRSIHIHDEYMWAPTTLHAGFSA
                     ELVTEIKKAVSIPVITVGRYTEPQFAELMVRQGRCDLVAFGRQSLADPEMPNKAKNGK
                     LDEMIPCIACLQGCVPNMFQGKPIACLANPILGHEAELKPAEISKEVLVVGGGVGGML
                     AAWVCAKRGHNVTLVEKSEVLGGQMRLAAYPPGKGDITNLVRSYISKCNQYGVKICTN
                     TEATVELIKEKSPDVVIIATGATPLVLPIPGINDSGLIHAVDLLDGKKSCGKKVLVVG
                     GGMVGCEVAAFLGEQEHEVTVIELREEVGADVISEHRKFLMNDFENYNIHTITGAKVS
                     KFYDDGVDYLLTDGTEHDLKGFDSVVLAMGSRNYDPLSEVIKEVVKETYIVGDAVKAR
                     RALDATKEAFEVALNI"
     gene            2995..3858
                     /gene="aroE"
                     /locus_tag="PC700_002368"
     CDS             2995..3858
                     /gene="aroE"
                     /locus_tag="PC700_002368"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDF2581670.1"
                     /translation="MEISGRTGLFALIGTPVGHSKSPVMYNYSFKKLDLDYRYLAFDI
                     TVDKVKEALLAIKTFNIKGANVTMPCKSAVTEYMDELSPAARIIGACNTIVNDNGKLV
                     GHITDGVGYVRNLKENGIEVKGKKITIMGAGGAATAIQVQCALDGAREISIFNAKDDF
                     YKRAEQTVENIKKDVPECVVNLYDLEDTNKLYEEIESSDILTNATLIGMKPYDNETNI
                     KDTSVLRKDLVVTDVVYNPKKTKMIEDAEANGCKAIGGLGMLLYQGAEAFNLYTGLEM
                     PVEEVNELCFK"
     gene            4058..5275
                     /locus_tag="PC700_002369"
     CDS             4058..5275
                     /locus_tag="PC700_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2581671.1"
                     /translation="MNNKKYYPTAIALYFSYFLLGIGISILGQYKPEFSSMWGAKTLS
                     DGTLDVSIVLAVIAALGLGRLISYPFAGPISDKYGRKVSGLIGNFLHAIFFVGIVFSP
                     NFYIAYVFAIIGGAANSFLDTCVTPSCMEIFASLGTIANMFTKFTIALAQFLLPFIIG
                     FVAANSISFKVIFIVTAILIVVDAILIAVLPFPPANNVIDNKGKTVKSEKMKFTPTSI
                     ALVCIGFTCTSTFVLWLNCNQELGKLYGMADPTKIQSFYSMGVICAVLITSLLIKKYI
                     KPIRILVIYPIIALLMLLIVYFVQTPTICMLGGFVIGYSAAGGVLQLTTSTANEMFPT
                     NKGKITSIVMIASSIANYVILNIAGIITKSGGVNGPKYVVLFNVAITFVGILLALFVN
                     MRYEKEKVYDYDV"
     gene            5511..7376
                     /locus_tag="PC700_002370"
     CDS             5511..7376
                     /locus_tag="PC700_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HDF2581672.1"
                     /translation="MENVFKRLQEFNGYDGYKESFEMNYLCIYESIPLREQVELANNL
                     VDEILNMYKSESNEIYLLEDSNSKSLICYFEIFMKKINTLVKEMIIDEKWLYKLTKEL
                     IYKSKKVEYVKLGLVLSEKYLNVENLREVVDTFSKSGEYVFYLSNTIKKLEFYNTYLF
                     NLSKKATGSIKVFAIVNMENLDSKINSYLIEDGYKDTKYERLLMNYIISIVDLNEYLE
                     KRDLDKEKINNLARLICNYLLSVEFKYIGNKLELVNRFLPTVVNYGTNFESLYSIFLI
                     AINVLKDENIEYNKIEFEKEINDILLSEKWKNIYFEALRDASGKTEDIIKMSEIYDVN
                     LSFDDLLPYLNRDIRDFEVYWHISKKGTTSSRLKLLNFFEETFKIDDLIGKMKDIEKD
                     KLTQEYYDDMLFFIVLKGSKSLYPEGKNISLKGIFGNINEVRKESINILKRYREKLSL
                     EELKIVKEAYEKEKNVILKDELRRVLYESNNLKKEFVNIEKIKVDEHGKDIYLTSIAV
                     AGSRFRNREYLEKELEKSKIYYLTREKDNLYDEKAIKIVGETGYVIGYVPRKENYILS
                     NLLDGGKLLYCRVTEYNLYEDCIYANVYLSYKDVIETVENSLKMVLDKSRIKLIN"
     gene            complement(7554..8918)
                     /locus_tag="PC700_002371"
     CDS             complement(7554..8918)
                     /locus_tag="PC700_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2581673.1"
                     /translation="MSQLLIKNAYLITMNADREVYETGSILIENNIIKNVGKFDINIV
                     DKDAEIYDAKGKILMPGLVNTHVHLSQQLGRGLADDVVLLTWLRERIWPYESSFNYED
                     SLISSTACCVELIKSGVTTFLESGGQYVDAMVEAVDKTGLRACLAKSVMDTGDGLPEA
                     WQKTTDEEINTQLDLFHKFNNTSNERIKIWFALRTIFNTTEELLIRTKELADKYNTGI
                     HMHIAEIADEISFVKQNNGVGTVEYLDKLGVLGPNLLAAHTVWLTHKEIDLFRLHDVK
                     VSHNPGAAMKVVLGFASIPEMLEKGIPVSIGTDGAPSNNRMDLMRDMYLTSLIHKGRT
                     LNPTVVPAEEVLEMATINGAKCALLEKEIGSLEVGKKADMIILNPDTIHCLPMHNPIG
                     NIVYSMTSENVDSTICDGKWLMKERKVLVVDESELLNKVKKQASKIKDKAEVKIPSKF
                     KIIK"
     gene            complement(9065..10408)
                     /locus_tag="PC700_002372"
     CDS             complement(9065..10408)
                     /locus_tag="PC700_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDF2581674.1"
                     /translation="MVPNTNNVQPDGNNSSLSFLDKMFKFSERGSSLKTEVIGGVTTF
                     LTMAYIVFVNPAILSEAGMDKAALITVTILATAIGTLIFAILGNAPFALAPGMGLNAF
                     FTYSLVIGAGIPWQTGLGVVFLSGCVFFTLAVTGLRKTIADAIPRELAVASSAGIGLF
                     LAFVGLKNMGVIIANPDTIVSLADFTPTVLIALFALILMAILEYKKVKGGILISIVVA
                     TIISVIFGFVELPTAIISTPPSIAPVAFKLDIVGALKFSLIGPIFSFMFIDMFDSLAF
                     LITCCKQMGLEDENGEIKDLGRMLYADVSSTLIGSFLGTSTVTTFGESASGIAAGART
                     GLASIVTAALFLATLIFTPLLGIVPTYAAAPALVMVGVFMFESIKQIDFADSKIAVPA
                     FVTVLLMPLTYSISIGLCFGFISYIIMHVVAKETDKISITLWIIGLLAVVNLVLS"
     regulatory      complement(10481..10582)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
BASE COUNT         3978 a         1357 c         1844 g         3583 t
ORIGIN      
        1 ataaaaatat aaaatcaaag atactattaa ttataaagaa aataaagggg gcaaatttaa
       61 gctatgaata atcaaaaaaa tcttaatatt ccaattacaa ttagttctta tacattggga
      121 acagaagttt cattccgtga tagagttcgt attgcaaaag aagctggatt tgatggtatt
      181 ggattgcgtg ctgaaaatta tattgatgca aaaaatgcag gtgttacaga tgaagaaatg
      241 attgcaattc ttaatgaata tgatatgaaa gttacagaag tagaatatat aactcaatgg
      301 ggaacagaag aagatagaac tgaagctcaa agagaaaaag agcaaaatgt ttatcatatg
      361 gctcacttat ttaatgttaa acatataaat tgtggattat tagagatgat accagaagaa
      421 caaattataa ctgcattggg agaattatgt gacagagcag gtgaacttat tataggtctt
      481 gagtttatgc catatagtgg tgtaccagat ttggctactg catggcgtgt tgtgaaagga
      541 tgtaatcgtg aaaatgcaat gcttatatta gatacttggc actgggctag agcaaagcag
      601 acagcagagg accttgcacc tgttccagca gataagatag tatcaattca agtgtgtgat
      661 gttttagaaa ctccttatga aaaacttagg gatgaatcac tacatgaccg tttggctcca
      721 ggtgaaggat atggaaacac agaggaattt gtgaaaataa taaaagaaca caatatatca
      781 ccaagagtca taggggcaga agtaatatct gattctctaa tatctaaagg gttatccact
      841 gctgctgaaa ctgtatttaa tgcaactaaa aaagttttgg ataaagcatg gtctgaagta
      901 tctccaaaat aaacttatat aatataaaaa tatacagagt attataatat aaaactttta
      961 aatataaaac ttgaaaggaa ggaaaacctt atgaagatta gttctatgtt tacacctata
     1021 agaattggtt caatgacagt tccaaatcgt tttgtagttc cccctatggg aaacaatttt
     1081 gcaaacacag acggtacttt aagtgaaact tctaaggctt attatcttga gcgtgcttta
     1141 ggtggttttg gattaataac tatagaatct agtgttgtag acaaaaaagc aaaaggaggt
     1201 cctcgtaagc cttgtttata tgatgatagc acaatagata gttttaaaaa tgtaatagat
     1261 gcttgtcatg atgcaggttc gaaggtatct atacaattac aacatgctgg ttctgaagga
     1321 aatgaaaaag tagcaggaca tccattaaaa gctgcttctg caatacctgc atcaaatggt
     1381 cgtaatacac cactagcaat aacaacagaa gaaatatatg aacttataga atcttatgga
     1441 gatgctgcat tacgtgcaca aaaagctggt gcagatgctg ttgaagttca ctgcgctcat
     1501 ggatatctgg ttagtagttt tatatcacaa agaacaaata agcgtgtaga tgagtttggt
     1561 ggatgttttg aaaatagaat gagattacct cgtcttatca ttgaaaatat acgtaaaaaa
     1621 gtaggaaatt ctctagcaat attatgtcgt ataaacagca ctgatgatgt tccaggagga
     1681 attgatatac atgatagttc agtaatagca gcatatctag aaggttgtgg aatagatgga
     1741 cttcatgttt cacgtagtat acatatacat gatgaatata tgtgggctcc tacgacttta
     1801 catgctggat tcagtgcaga attagttact gaaataaaaa aagctgttag tataccagta
     1861 ataacagttg gacgttatac agagccacag tttgctgagt taatggtcag acaaggaaga
     1921 tgtgatttag tagcattcgg tagacaaagc ttggctgacc cagaaatgcc taataaagct
     1981 aaaaatggaa aactagacga aatgatacca tgtattgcat gcttacaagg atgtgtacca
     2041 aatatgttcc aaggaaaacc tatagcctgt cttgcaaatc ctatattagg acatgaagca
     2101 gagcttaagc cagctgaaat tagcaaagaa gttcttgttg ttggtggagg tgttggaggt
     2161 atgcttgcag catgggtatg tgctaaaaga ggacataatg taactttagt tgaaaaatct
     2221 gaagttcttg gtgggcaaat gcgtttagct gcatatcctc cagggaaagg agatattact
     2281 aacttagtta gaagttatat ttcaaaatgt aatcaatatg gtgtaaaaat atgtactaat
     2341 acagaagcta cagtagaact tataaaggag aaatcacctg atgtagttat tatagctact
     2401 ggagcaactc cacttgtact tcctatacca ggaatcaatg attcaggatt aattcatgct
     2461 gtagaccttc ttgatggtaa aaaatcttgt gggaaaaaag tacttgttgt tggaggtgga
     2521 atggttggat gtgaagtggc agccttcctt ggagaacaag aacatgaggt tactgttatt
     2581 gagcttagag aagaagttgg tgcagatgta atttcagaac accgtaaatt ccttatgaat
     2641 gattttgaaa attataatat acataccatt actggtgcaa aagtttctaa attttatgat
     2701 gatggtgtcg actatttact tacagatggc acagagcatg atttaaaagg ttttgattca
     2761 gtagtacttg ctatgggttc tcgtaattac gacccattaa gtgaagttat aaaagaagtt
     2821 gtaaaagaaa cttatattgt tggtgatgca gtaaaagctc gtagggcatt agatgcaaca
     2881 aaagaagctt ttgaagtagc attaaatata taaataataa ataaattttt ctttgtaaat
     2941 aactgaatag gttatttgca aagaaaatga taatattggg aggaaattat agttatggaa
     3001 atttcaggta gaacaggatt atttgcatta ataggtacac cagttggtca ctctaaatca
     3061 ccagtgatgt ataattatag ttttaagaaa ttagatttag attatagata tttagccttt
     3121 gatatcacag tagataaggt aaaagaggca cttttagcaa taaaaacttt taatataaaa
     3181 ggagccaatg ttactatgcc atgtaaatct gctgttacag aatatatgga tgaactttct
     3241 ccagcagctc gtataattgg agcatgtaat acaattgtaa atgacaatgg aaagttagtt
     3301 ggtcatataa cagatggagt aggctatgtt cgtaatttaa aagaaaatgg gatagaagtt
     3361 aaaggtaaaa agattacaat tatgggtgct ggtggtgcag caactgctat acaagtacaa
     3421 tgtgctttag atggagcaag agaaatatct atctttaatg ctaaggatga tttttataaa
     3481 agagcagagc agacagttga gaatataaaa aaagatgtac cagaatgtgt tgttaatcta
     3541 tatgatttag aagatacaaa caaactttat gaggagatag aatcaagtga tatattgaca
     3601 aatgcaactc ttattggtat gaagccttat gacaatgaaa caaatataaa agatactagt
     3661 gttttaagaa aagacttagt agtaacagat gtcgtttata atcctaagaa aacaaaaatg
     3721 attgaagatg ctgaagctaa tggatgtaag gctattggtg gattaggaat gttattatac
     3781 caaggagcag aagcttttaa tctatatact ggattagaaa tgcctgtaga agaagttaat
     3841 gaattatgct ttaaatagat aagagtgata tcttaaacaa tgaaaaaaca tacgtatttc
     3901 aatattttta ataataaatt acaaattgtc atagtgggtt ttgcatcata gtccagttaa
     3961 atttgaagct ttaactgact atactttaaa tccattatga ggatgagttt attgtacaca
     4021 aaaatgttaa taaattaaca agaatgggag aaaaattatg aacaataaaa aatattatcc
     4081 tactgccata gcgttgtatt tttcatattt tttacttggt attggaattt caattttagg
     4141 acaatacaaa ccagaatttt catcaatgtg gggggctaaa acactatcag atggaacatt
     4201 ggatgtaagt attgttttag ctgttattgc tgcactagga ttagggagac ttatatctta
     4261 tccatttgca ggacctattt cagataagta tggaagaaaa gtaagtggtc ttataggaaa
     4321 ctttctacat gcaattttct ttgttggaat tgtattttca ccaaattttt atatagcata
     4381 tgtatttgca ataattggag gggcagcaaa ttcattctta gatacctgtg taactccttc
     4441 ttgtatggag atatttgcta gtttaggaac aatagcaaat atgtttacta aatttacaat
     4501 agctttagca caatttttgc ttccttttat aattggattt gttgcagcaa attcaatatc
     4561 atttaaagtt atttttatag taacagcgat tcttattgta gtagatgcca ttttgatagc
     4621 tgtgttacca tttccaccag ctaataatgt tattgataac aaaggcaaaa cagttaaatc
     4681 agagaagatg aaatttacac caacaagtat agctcttgtt tgtattggat ttacatgtac
     4741 atctacattt gtattatggt taaattgtaa tcaggaattg ggtaaattgt atgggatggc
     4801 tgaccctaca aaaattcaat ccttttactc aatgggagta atatgtgcag tattaataac
     4861 ttctttactt attaaaaaat atattaaacc aataagaata ttagttatat atcctataat
     4921 agctttactt atgctattaa ttgtatattt tgttcaaact ccaactattt gtatgttagg
     4981 gggatttgta ataggttatt cagcagcagg aggtgttctt cagttaacaa cgtcaactgc
     5041 aaatgaaatg tttccaacta ataaaggaaa aataacatct atagttatga ttgcatctag
     5101 tatagcaaac tatgttattt taaatatagc aggtattatt acaaaatcag ggggagttaa
     5161 tggaccaaaa tatgttgtat tgtttaatgt ggcaataaca tttgttggaa ttttacttgc
     5221 attgtttgta aacatgagat atgaaaaaga aaaagtgtat gattatgatg tctaatatta
     5281 gtataatata tgcatagaga agtctaaatc taggatagat ttttattaaa agtaattaaa
     5341 attaataagg atattattta cagttataag atagggtatt tacataatta ataataagga
     5401 tattactaga atttcaattt ctagtaatat ttttttgcgt ataaatgtaa acttgacgaa
     5461 ataagtagta taatcaatat aaattacatc atgtaaagga ggataatttg gtggagaatg
     5521 tttttaagcg attgcaggaa tttaatggtt atgatggata caaagaatca tttgaaatga
     5581 attacctatg tatatatgaa agtataccgt taagagaaca agtagaactt gcaaataatc
     5641 tagtagatga aattttaaat atgtataaaa gtgaatctaa tgaaatttat ttattggagg
     5701 atagtaattc taagtcttta atttgttatt tcgaaatttt tatgaaaaag ataaatactt
     5761 tggtaaaaga aatgattatt gatgagaaat ggttatataa attaactaaa gaattaatat
     5821 ataagagtaa aaaagttgag tatgtaaaac tagggttagt tttaagtgag aaatatctaa
     5881 atgtagaaaa cttaagagaa gttgtagata cattttctaa atctggagaa tacgtatttt
     5941 atctgagtaa tactattaaa aaacttgaat tttacaatac atatttattt aatttatcta
     6001 aaaaggctac aggttcaata aaagtatttg ctattgttaa tatggaaaat ttagattcta
     6061 aaataaattc ctatctaatt gaagatggtt ataaggatac aaagtacgaa cgattattga
     6121 tgaactatat tatttcaatt gtagatttga atgaatatct tgaaaaacga gatttagata
     6181 aagaaaagat aaataattta gcacgtctta tatgcaatta cttgctaagt gtagaattta
     6241 aatatatagg taataaatta gaattagtga atcgattttt acctactgta gtaaattatg
     6301 ggactaactt tgaaagttta tattcaattt tcctaatagc tataaacgtc ttaaaagatg
     6361 aaaatataga gtacaataag attgaatttg aaaaagaaat aaatgatatc ttgctcagtg
     6421 aaaaatggaa aaatatatat tttgaagcat taagagatgc tagtggaaaa acagaagata
     6481 ttataaaaat gtcagaaatt tatgatgtaa acttatcttt tgatgacttg ttgccatatc
     6541 tgaatagaga tattagagat tttgaggtat attggcatat atctaaaaag ggaacgacaa
     6601 gttctagatt aaaattatta aatttttttg aagagacgtt taaaatagat gatttaattg
     6661 gaaaaatgaa agatatagaa aaagataaat tgactcaaga atattatgat gatatgttat
     6721 tttttattgt gcttaaagga agtaaatcac tatatcctga aggaaagaat atatctttaa
     6781 aaggaatatt tggaaatata aatgaagtaa gaaaagaatc cataaatatc ttaaaaagat
     6841 acagagagaa actatctctt gaagaattaa aaattgttaa agaagcgtat gaaaaagaaa
     6901 aaaatgttat attgaaggat gaattaagaa gagtattata tgaaagtaat aatttaaaaa
     6961 aagaatttgt aaatattgaa aaaataaaag tagatgaaca tggtaaggat atctatttga
     7021 cttcaatagc tgttgctggt tcaaggttta gaaatagaga atatcttgaa aaagaattgg
     7081 agaagagtaa gatatactat ttaactagag agaaagataa tctatatgat gaaaaagcta
     7141 ttaaaatagt tggtgaaact ggatatgtaa taggctatgt cccaagaaaa gaaaactata
     7201 ttttgagcaa tttattagat ggaggaaaac tgctgtattg tagagtaact gaatataatt
     7261 tatatgaaga ttgtatctac gcaaatgtgt acttgagtta taaagatgta attgaaacag
     7321 ttgaaaactc tttaaagatg gttttagata agagtagaat aaaacttata aactaagacg
     7381 tttatatatt gcttagatga tgtaatccta actgattggt tattttttct ggaattatgg
     7441 tgtattttat gagcaatgtt aatgaatata aaataaaaaa acaattgttt tgtatatatg
     7501 atgtgtataa atatatttat atattcatat ttacaaaaca attgaaattt atgttatttt
     7561 atgattttaa atttagatgg aattttaact tctgctttat cttttatttt agaagcttgt
     7621 tttttaactt tatttaataa ttcagattca tctacaacta atacttttct ttccttcatc
     7681 aaccatttac catcacaaat agtagaatct acattttcac tagtcataga ataaactata
     7741 tttccaattg gattatgcat tggtaaacaa tgaattgtat ctggatttag aataatcata
     7801 tcagcttttt ttcctacttc aagactacca atttcttttt ctagtaaagc acattttgct
     7861 ccatttatag tagccatttc taagacttct tcagctggta cgacagttgg gtttaaagtt
     7921 ctacctttat gtattagtga tgtaagatac atatccctca taaggtccat tctattattt
     7981 gaaggagctc catcagtacc aattgaaaca ggtattcctt tttctaacat ttcaggaatt
     8041 gaggcaaatc ctagaactac tttcatagct gcacctggat tatgagaaac ttttacatca
     8101 tgtaatctaa ataaatcaat ttctttatga gtaagccaaa ctgtgtgagc agctaaaaga
     8161 tttggtccta atacacctag tttatcaaga tattcaactg ttccaactcc gttgttttgc
     8221 tttacaaatg aaatttcatc agctatttca gcaatatgca tatgtatacc tgtattgtat
     8281 ttatcagcta actctttagt cctaataagc aattcttcag ttgtattaaa aatagttctt
     8341 aatgcaaacc atattttaat cctctcatta gatgtgttat taaacttatg gaataagtct
     8401 aattgagtgt tgatttcctc atcagtagtt ttttgccatg cttcaggaag accatctcct
     8461 gtatccataa ctgatttagc taaacaggct ctaagtccag ttttatcaac agcttcaacc
     8521 atagcatcta catattgacc accagattct aaaaatgtag ttaccccaga ttttatcaat
     8581 tcaacacaac atgcagtaga tgaaatcaat gaatcttcat agttgaagct actttcgtat
     8641 ggccagattc tctctctaag ccaagttaaa agtacaacat catctgcaag accacgtcct
     8701 aattgttggg ataagtgaac gtgtgtattg actaaccctg gcattagtat ttttccttta
     8761 gcatcatata tctcagcatc tttatctaca atattaatat caaacttacc aacatttttt
     8821 ataatattat tttcaataag tatacttcca gtttcatata cctctctatc tgcattcata
     8881 gtaattagat atgcattttt tataagtaat tgtgacataa gtacaacctc ttttctatat
     8941 atttttatat ctatgtatta tgataataaa ttttctatac aaagttaata gcaaaatcaa
     9001 tttatacata aattgatttt gctatttata aatttaaaat attgacctag aactatttaa
     9061 aatattaact tagaactaag tttacaactg caagtaaacc tataatccaa agtgtaatac
     9121 tgattttatc agtttcttta gctaccacat gcattattat atatgaaata aatccaaagc
     9181 ataaaccaat acttatacta taagttaaag gcatcaataa aactgtaaca aaagcaggta
     9241 cagctatttt gctatcagca aaatcaatct gttttatgct ttcaaacatg aatacaccaa
     9301 ccattacaag tgcaggagct gcagcgtaag taggaactat accaagcaat ggtgtaaata
     9361 taagtgtagc taagaataat gctgctgtta ctattgaagc aagtcctgtt cttgcacctg
     9421 ctgcgatacc agaagcagat tctccaaatg ttgtaacagt actagttcct aagaatgagc
     9481 ctattaaagt agaagaaaca tctgcatata acattcttcc taaatctttt atttcaccat
     9541 tctcatcttc aagacccatt tgtttacaac aagtaataag aaatgctaat gaatcaaaca
     9601 tgtctataaa catgaaagag aatataggtc caattaatga gaatttcaat gcacctacaa
     9661 tatctaattt aaaagcaact ggtgctatac taggaggtgt tgagattatt gctgttggca
     9721 actccacaaa tccaaatata acagatatta ttgttgcaac tactatactt atcaatattc
     9781 cacctttaac ttttttatat tctagtattg ccattaagat aagagcaaaa agagctatta
     9841 aaactgttgg tgtaaaatct gcaagtgaaa ctatagtatc tggattagct attataacac
     9901 ccatattttt taaaccaaca aaagcaagga aaagacctat acctgctgaa gatgctactg
     9961 ccaattctct aggaattgcg tctgcaattg tctttcgaag acctgttact gcgagtgtaa
    10021 agaagacaca tccagataga aaaactaccc caagacctgt ttgccatggg attcctgcac
    10081 ctataaccaa agagtatgtg aaaaatgcat ttaatcccat accaggagct agagcaaatg
    10141 gtgcatttcc taaaatcgca aatataagtg taccaatggc tgtagctagt atggtaactg
    10201 ttataagggc ggctttatcc attccagctt cagataatat tgctggattt acgaaaacta
    10261 tgtatgccat tgttaagaat gtagtaactc cacctattac ttctgtttta aggctagagc
    10321 cacgttcgct aaatttaaac attttgtcta aaaagcttaa tgatgaatta tttccatctg
    10381 gttgtacatt gttagtgttt ggtaccattt ttacctccaa aaaactttaa gatataaaca
    10441 aaaaaatata tattcatata gatagaatag aacatatatt caaagttgat attctattca
    10501 tagaaaaccc atttaaggtg agtttgtaga aactattaga ccgtattact aatattatat
    10561 gaacattttc tatttgtttt tctgttgtat tatatcgtaa tattgtatta tttacaataa
    10621 atataataaa attattcgtt tttttgtcga ttttataaaa aattaaggtt atttattggc
    10681 aagaattact gttttcaata attgaatatg tataagtata atgtcaatta ttctaacttg
    10741 ttttatacta ttatatagaa gt
//
LOCUS       DAMJWP010000116        10669 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.121, whole genome shotgun sequence.
ACCESSION   DAMJWP010000116 DAMJWP010000000
VERSION     DAMJWP010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10669
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.121"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            119..1813
                     /locus_tag="PC700_002373"
     CDS             119..1813
                     /locus_tag="PC700_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HDF2581675.1"
                     /translation="MKFSEFKYERPNYNSMKKEFLSCVEDINNSRNYKEQQKNIHKIN
                     LLRNKIETLSNIASIRYSTDTFNKFYKEEKNYWDEYMPLYEELNSHFYNAIVNSKFKY
                     DLIKEFGEQFFTIVEYSLKSFSKEIISELQEENKLCSEYTRLLASAEIMFDGNIRNLS
                     GMGKFMYSKNRKTRELANKAYYNFFEENETKFDDIFDKLVKLRDKISKKLGFEDFVEL
                     GYVRMMRSDYREDMIKNVRKQVLKYVVPMANELYEKQAKRIGLEYLSYIDEGVEFLTG
                     NASLKGDSRYIIQNGKRMYSELSKETNEFFDFMLENELMDLETKKGKGAGGYCTYIPD
                     YKSPFIFSNFNQTADDIDVLTHEAGHAFQLYMSRWIDMPEINFPTLDSCEIHSMSMEF
                     ITWPWMDLFFKEDTDKYKFTHLSSSIKFIPYGVIVDEFQHYIYKNPNVDKSKRKEIWR
                     FLEKKYLPHRKYGDNSFLERGCWWFKQGHIFKNPFYYIDYVLAQICALQFWKKMIQDR
                     DAGWKDYINICKVGGTKSFLDIVSMGNLYSPFDDGCIESIIGDVKSWFDEINDSKL"
     regulatory      2145..2255
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            2359..2730
                     /locus_tag="PC700_002374"
     CDS             2359..2730
                     /locus_tag="PC700_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HDF2581676.1"
                     /translation="MIIITNNPKVKEEVQGREVLFKDTTYIGILEASRDLIHEGYELL
                     SHPLYGSVKPNETPYRTVILKKGNRLDINSLTLIEEAIITASKFQDNKKTPKWTENVQ
                     DDFRVIDYDIFYNTIQRMQYE"
     gene            2826..3773
                     /gene="trxB"
                     /locus_tag="PC700_002375"
     CDS             2826..3773
                     /gene="trxB"
                     /locus_tag="PC700_002375"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDF2581677.1"
                     /translation="MENVYDLVIIGSGPAGLAAGLYGARAKLKTLILEKDKTGGQIVI
                     THEIANYPGSVPNATGPSLIARMVEQCKEFGAEMLRDNIVDTELDGDIKVLKGEKAEY
                     RAKAVIIGTGATPRKIGCPGEKELTGKGVSYCATCDADFFEDFEVFVVGGGDSALEEA
                     MYLTKFARKVTIVHRRQGFRCAKSVEEKAKANPKIEFLLDTVIEEIKGDGILESVVFK
                     NKVTGETHEYFADEEDGTMGVFVFVGLDAQTDLFKGKVDMDEKGYIITDEDMRTNIPG
                     VFAAGDCRSKTLRQVVTATNDGAIASIVAEKYIDEKFGN"
     gene            3858..4175
                     /locus_tag="PC700_002376"
     CDS             3858..4175
                     /locus_tag="PC700_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HDF2581678.1"
                     /translation="MLDLDKATFEEEVLNAEGFVFVDFWSEGCEPCKALMPDVHKLAE
                     TYGDKIKFCKMDTTKARRLAIKQKVLGLPTMAIYKDGEKVDEVTKDDATVPNIENMIK
                     KYL"
     gene            4444..5730
                     /locus_tag="PC700_002377"
     CDS             4444..5730
                     /locus_tag="PC700_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HDF2581679.1"
                     /translation="MRLELGKIFIKDIQFGDVTEVKDGVLFINKQEMLQEIGGDEHIK
                     SIDIELARPGESVRITPVKDVIEPRVKVEGNGGIFPGIMSKVDTVGEGKTHALKGVAV
                     VTTGKIVGFQEGIIDMTGEGAKYTPFSKLNNVVVIAEPIDGLKQYAHEKAVRMIGFKA
                     AMYLGEVARNLTPDEVSVYETKPLLESIKEYPELPKVGYVYMLQTQGLLHDTYVYGVD
                     AKQIVPTLLYPTELMDGAIVSGNCVSACDKNPSYVHINNGVVEDLYARHGKDINFVGV
                     IITNENVYLADKERSSNWTSKLCKYLGLDAVIVSQEGFGNPDTDLIMNCKKIEMQGVK
                     TVIVTDEYAGRDGGSQSLADADVRADAVVTGGNANQVVVLPKLDKVIGHLEVVDVIAG
                     GSDGSLRADGTIEVEIQAITGATNETGFGHLTAKGY"
     gene            5842..6315
                     /locus_tag="PC700_002378"
     CDS             5842..6315
                     /locus_tag="PC700_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:5974..5976,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HDF2581680.1"
                     /translation="MSLLSNKKVLIIGDRDGIPGPAIEECVKTVEGAEVVFSSTECFV
                     UTAAGAMDLENQNRVKDAADKFGAENVVILLGAAEAEAAGLAAETVTAGDPTFAGPLA
                     GVALGLSVYHVVEEPIKSLFDESVYEDQISMMEMVLEVEEIEEEMSGIREEFCKF"
     gene            6347..7657
                     /gene="grdB"
                     /locus_tag="PC700_002379"
     CDS             6347..7657
                     /gene="grdB"
                     /locus_tag="PC700_002379"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:7394..7396,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HDF2581681.1"
                     /translation="MGKLKAVHYINQFFAGIGGEEKADTKPHVAETLPPISLQLDKLL
                     GEDIEIVGTVVCGDSYFNENIDSASEEVLSMVKGFEPQLFIAGPAFNAGRYGVAAGTI
                     TKVVKDALNIPALTGMYVENPGADMFKKDVYVVETSDSAAGMRKALPKIAKLAVKLAN
                     GEEIGTPKDEGYIARGIRVNYFHEDRGSKRAVDMLVKKIKGEPFETEYPMPNFDRVDP
                     SKAVKDLSKCKIALVTSGGIVPKGNPDRIESSSASKYGTYSIAGVMDLTEETYETAHG
                     GYDPVYANLDADRVLPVDVLRDLEKEGVIGKLHETFYTTVGNGTSVANSKKYASEIGA
                     ALVADGVDAVILTSTUGTCTRCGATMVKEIEKTGLPVVHMCTVVPISLTVGANRIVPT
                     IAIPHPLGNPALDPTEEKALRRGLVEKALNALTTEVDGQTVFEK"
     gene            7914..9446
                     /locus_tag="PC700_002380"
     CDS             7914..9446
                     /locus_tag="PC700_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III family protein"
                     /protein_id="HDF2581682.1"
                     /translation="MTYPVLKGAGYVLIHTPDMIVQNGSTCTVERATNSDSEFLKEVS
                     NHIRSYEDVVNYMPNQVYIGNRRPEELRDLPMPWCEQKIEGTRNGKFGEIMPQDEFIA
                     LMQISDAFDLVKLSQEFIDEVKPKIENNYPEIAPFVGKLKGDDIEEGKELVATHIAEG
                     LYHDGKFVGYVKRAHDVDVNLNAHTMFENLVVKASGVLSAIQMLRHSKIDPAEIDYVI
                     ECSEEACGDINQRGGGNFAKSIAEIAGLQNATGSDTRGFCAAPTHALIQAAALVKAGI
                     HKNVMVVAGGASAKLGMNAKDHVKKGLPVLEDVVGGFAVLVSENDGVNPVIRTDLTGK
                     HTVGTGSSPQAVMTALITSGLDRANLKITDVDVYSVEMQNPDITKPAGAGDVPEANYK
                     MIGALAVKRGDLEKKELKDFVSNKGLPGWAPTQGHIPSGAPYIGFLIDDLTTGNRNRA
                     MIVGKGSLFLGRMTNLFDGVSFIAERNTGVTEETSGISKDEIKKIIAESMKKLALDML
                     EE"
     gene            9457..10602
                     /locus_tag="PC700_002381"
     CDS             9457..10602
                     /locus_tag="PC700_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HDF2581683.1"
                     /translation="MSKKVIADVFLEVANAIESGEFGKKVKIGVTTLGSEHGVENMVN
                     GAQLAKSNLFDIVLIGPKVETDLEVVEVNDEKEMHAKMEELLDSGYIDACVTMHYNFP
                     IGVSTVGRVITPAKGKEMILATTTGTSATNRIEAMVRNAIYGIATAKSMGNKCPKVGI
                     LNVDGARQVEKCLKELKDNGYDMEFADSIRADGGCVMRGNDLLVGAPDVMVTDTLSGN
                     IFMKVFSSYTTGGDYEAQGFGYGPGVGEDYDRKVLIVSRASGSPVVANALKYAYDVVK
                     GDISNVARNEFAKVKKAKFDDIISSLTKKEVKAEKVEVKMPDKEIVTRQIAGVDIMDL
                     EDAVSELWKNGIYAESGMGCTGPIVLVNEAKGDLAVETLVKAGYTAK"
BASE COUNT         4232 a         1165 c         2120 g         3152 t
ORIGIN      
        1 atgattataa tataaagaat atttaatata tataataatt tataaaaaag tattacatag
       61 tacatgtaaa tctatattta taaatacaat aaagtaaagt tgtaaaggag tgctttaaat
      121 gaagttttca gagtttaagt atgaaagacc caattataat agtatgaaaa aagagttttt
      181 gagttgtgta gaagatataa ataattctcg taattataaa gagcaacaaa aaaatataca
      241 taagattaat ttattaagaa ataaaataga aacgttgtca aatatagctt cgataagata
      301 tagtacagac acatttaata aattctacaa agaagaaaaa aattattggg atgaatatat
      361 gcctttgtat gaagaattaa attcacattt ttataatgct attgtaaatt caaaatttaa
      421 atatgatttg ataaaagagt ttggagaaca atttttcaca atagtagagt attctttaaa
      481 aagtttttct aaagaaataa taagtgaatt gcaggaagaa aataaattgt gttcagagta
      541 tactagactt ttagcttcag ctgagataat gtttgatggg aatataagaa atttatctgg
      601 tatggggaaa ttcatgtatt ctaagaatag aaaaacaaga gaattagcaa acaaagcata
      661 ttataatttc tttgaagaaa atgaaactaa atttgatgac atatttgata aattggttaa
      721 acttagagat aagatatcta aaaaactagg ttttgaagat tttgtagagc ttgggtacgt
      781 tagaatgatg agaagtgact acagagaaga tatgataaaa aatgttagaa aacaagtatt
      841 aaaatatgtt gttccaatgg ccaatgagct ttatgaaaaa caagctaaaa gaattggtct
      901 agaatattta agttacatag atgaaggcgt ggaattttta acaggaaatg caagtttaaa
      961 gggagattca agatacataa ttcaaaatgg taaaaggatg tattctgaac tgtcaaaaga
     1021 aacaaatgaa ttttttgatt ttatgttaga aaatgaactt atggatttag aaacgaaaaa
     1081 aggaaaagga gctggagggt attgtactta tataccagat tataagtcac cattcatttt
     1141 ttctaatttt aatcaaactg ctgatgatat agatgtgtta acacatgagg caggacatgc
     1201 atttcagttg tatatgtcta gatggattga tatgccagaa attaattttc caacgcttga
     1261 tagttgtgaa atccattcaa tgagtatgga atttataaca tggccgtgga tggatttatt
     1321 ttttaaagaa gatacagata agtacaaatt cactcactta tcatcttcaa ttaaatttat
     1381 accttatgga gtaattgttg atgaatttca acactatatt tacaaaaatc ctaatgtaga
     1441 taagtcaaaa cgaaaagaaa tctggagatt tttagagaaa aaatacttac ctcatagaaa
     1501 atatggtgat aattcttttt tagagagagg ttgttggtgg tttaaacaag gacatatatt
     1561 taaaaatccg ttttattata tagattatgt attagctcaa atttgtgcgt tacaattttg
     1621 gaaaaaaatg attcaagata gagatgcagg atggaaagat tatataaata tctgtaaagt
     1681 tggaggaaca aaatcatttt tagatattgt gagtatggga aatttgtatt ctccatttga
     1741 tgatggctgt attgagtcta ttataggtga tgttaaatct tggtttgatg aaataaatga
     1801 cagcaaatta taaaaaatta tgagaatttt attgtataaa agtgaaaaat taaaaaatat
     1861 ataaaaagtg attatttgtt gtgaaaatag tcttgttttt aactaaattg ataaaataaa
     1921 ctaaaaaaga tttgtactta aaaaaaaaag aaaatacaag aaatatgcaa tatcaacatt
     1981 tggaaaaaaa agtaaaaatg tatacaaaat acatataaaa aaactattgc aaattttata
     2041 gaatgtttat atacttaagt caacagagat agaaaaaagc tatttataga aaacaaagga
     2101 taaattgctt aattttaaat tttctgaaaa aaataataat ccggatgaag agtatgggag
     2161 actgctttat aaaaagcgac cgaagggaca agtttaatct tacttaccca aaagattaga
     2221 tgaaattctc aggtaaaggt gactgtactt ggacggggct ctgaaaatga ttttaacagg
     2281 gaagacaaat tgggagattt gtttttcctg ttttttattt atcaagtaat aaataaaagt
     2341 ttgaaagaga gtaaaaaaat gataataatt acgaataatc caaaagtaaa agaagaagtt
     2401 caaggcagag aagttttgtt taaagacaca acttatattg gaatcttaga agcaagtaga
     2461 gatttgatac atgaaggtta tgaactttta tctcatccac tctatgggag tgtaaaacca
     2521 aatgaaacac cttacaggac tgttatacta aagaaaggaa atcgtttgga tataaactct
     2581 ttaaccttaa ttgaagaagc aatcatcact gcaagtaaat ttcaggataa taaaaagact
     2641 ccaaaatgga cagaaaatgt ccaagacgat tttagagtaa ttgattatga tatattttat
     2701 aatacaatac aaagaatgca gtatgaataa aaaaatttgt taaaaagtta ttgaaacagc
     2761 ctaaatatta aatctaattt aatattaaat aaaatgagaa atttaattgg ggaggataag
     2821 tcaaaatgga aaatgtgtat gacttagtaa taattggttc aggaccagct gggcttgcag
     2881 caggtcttta tggagcaaga gcaaagttaa aaactctaat cctagaaaaa gacaagactg
     2941 gtggacaaat tgtaataaca catgaaattg caaattatcc agggtcagtt ccaaatgcaa
     3001 caggaccaag tttaatagct agaatggttg aacagtgtaa agagtttgga gcagaaatgc
     3061 taagagataa cattgttgat actgagctag atggagatat aaaagtttta aaaggggaaa
     3121 aggcagaata tagagcaaaa gcagtaataa taggaacagg agcaacacca agaaagattg
     3181 gttgtcctgg agaaaaagaa cttactggta aaggggtttc atattgtgca acttgtgatg
     3241 cagatttctt tgaagatttt gaagtatttg ttgtaggtgg aggagacagt gcattagaag
     3301 aagcaatgta tttaactaaa tttgctagaa aagtaacaat tgtacataga agacaaggat
     3361 ttagatgtgc taagagtgtt gaagaaaaag caaaagctaa tccaaagata gagtttttat
     3421 tagatacagt aattgaagaa atcaaaggtg atggaatatt agaatcagtt gtatttaaaa
     3481 ataaagttac tggagaaact catgaatact ttgcagatga agaagatgga acaatgggag
     3541 tatttgtgtt cgttggatta gatgctcaaa ctgatttatt caaaggtaaa gttgatatgg
     3601 atgaaaaagg ttacataata actgatgaag atatgagaac taacatacca ggcgtatttg
     3661 cagcaggaga ttgtagatct aagactctaa gacaggttgt aactgcaact aatgatggcg
     3721 caattgcatc tatagtagct gagaaataca ttgatgaaaa atttgggaac tagaatctgt
     3781 aaattaagaa atagtgtctg taaatttaat gaataatatt taaaattaaa ataaaaattt
     3841 caagggggaa taagaaaatg ttagatttag ataaggctac atttgaagaa gaagttttaa
     3901 atgcagaagg ttttgtattt gttgattttt ggagtgaagg ttgtgagcct tgtaaagctt
     3961 taatgccaga tgttcataaa ttagctgaga cttatgggga taaaataaaa ttctgtaaga
     4021 tggatacaac aaaagctaga agattagcta taaaacaaaa agtattaggt cttccaacta
     4081 tggctatata caaagatgga gaaaaagtag acgaagttac taaggatgat gctacagttc
     4141 ctaatataga aaacatgatt aaaaaatatc tttaatatgt tgataaagta ttaacattaa
     4201 aagaaaaaat ctatttttat ctgaagataa gaagaaaaag gtttttatgt ccaactttgt
     4261 taaaaaaata attttaaata aaataatttt tttacaataa atatttttta gtaataaaat
     4321 aaagatttaa ggcataaaaa cacaataata tatctttaaa aaaataagat aaattgtaaa
     4381 atgatataat tttgagaaaa actcaaatgc atatacacaa attaaataac aggaggtgca
     4441 agtatgcgtc ttgaattagg gaaaatcttt ataaaagata ttcaatttgg tgatgtaaca
     4501 gaagtaaaag atggagtatt attcatcaat aagcaagaga tgctacaaga aattggtgga
     4561 gatgagcata ttaaatctat agatatagaa ttagctcgtc caggtgaaag cgtaagaata
     4621 acacctgtaa aagatgttat tgagccaaga gtaaaagttg aaggtaatgg gggaatcttc
     4681 cctggtatca tgtctaaagt tgatactgta ggagaaggaa aaactcatgc tttaaaaggt
     4741 gtagctgttg taactactgg aaaaatagta ggtttccaag aaggtataat agacatgact
     4801 ggagaaggag ctaagtatac tccattttct aaattaaata atgtagtagt aatagctgaa
     4861 cctattgatg gtttaaaaca atacgctcat gaaaaagctg ttagaatgat aggattcaaa
     4921 gcagcaatgt acttaggaga agtagcaaga aatcttactc cagatgaagt ttcagtatat
     4981 gaaactaaac ctcttcttga atcaataaaa gaatatccag agcttccaaa agtaggatat
     5041 gtttacatgc ttcagactca aggtttatta catgatactt atgtatatgg agtagatgca
     5101 aaacaaatag taccaacttt attatatcca acagaattaa tggacggagc aattgtaagt
     5161 ggtaactgtg tttctgcatg tgacaaaaac ccaagttatg ttcatataaa caatggggtt
     5221 gttgaagact tatatgcacg acatggtaaa gatataaact ttgtaggtgt aattataact
     5281 aatgaaaatg tttacctagc agataaagaa agatcttcaa actggacttc taaattatgt
     5341 aaatatttag gattagatgc agttatagtt tcacaagaag gttttggaaa tcctgatact
     5401 gaccttataa tgaactgtaa gaagatagaa atgcaaggtg ttaaaacagt tatagttaca
     5461 gatgaatatg ctggacgtga tggaggttct caatcacttg cagatgcaga tgtaagagct
     5521 gatgctgttg taacaggtgg aaatgccaac caagttgtag ttcttcctaa gttagataaa
     5581 gttattggac atttagaagt agtagatgta atagctggtg gttctgatgg aagtttaaga
     5641 gctgatggaa ctattgaagt tgaaatccaa gctataacag gtgcaactaa tgaaacagga
     5701 tttggtcatt taactgctaa aggatattaa gaagtatttt attaaacgat tgagtaatgt
     5761 taataaaaca tattatagat aattataata agtgttatag atatataaaa atatattaga
     5821 aatataataa ggaggattat tatgagttta cttagtaata aaaaggttct tataataggt
     5881 gaccgtgatg gtataccagg acctgcgata gaagaatgtg taaaaacagt agaaggagca
     5941 gaggttgttt tctcatctac agaatgcttt gtctgaacag ctgctggggc tatggactta
     6001 gaaaatcaaa acagagttaa agatgctgct gataaattcg gagctgaaaa tgttgtgatt
     6061 ttactaggtg ctgctgaagc cgaagctgca ggtcttgcag ccgaaacagt aactgctgga
     6121 gatccaactt tcgctggacc acttgctgga gttgccttag gattaagtgt ttaccacgtt
     6181 gttgaggaac caataaaatc attatttgat gaaagtgtat atgaagacca aataagtatg
     6241 atggaaatgg ttttagaagt tgaagaaata gaagaagaaa tgtctggtat aagagaagaa
     6301 ttttgtaaat tttaatgcct aaaagcaaaa ggggtgaaac taagagatgg gaaaactaaa
     6361 agcagtacat tatattaatc agttctttgc tggtataggt ggagaagaaa aagctgatac
     6421 taagccacat gtggcagaaa ctttacctcc aataagttta caacttgata aattattagg
     6481 tgaagatatt gaaatagttg gaacagtagt ttgtggagat agctatttca atgaaaatat
     6541 tgatagtgca agtgaagaag ttttatcaat ggtaaaaggt tttgaaccac aacttttcat
     6601 agctggtcct gcatttaatg ctggtagata tggggttgct gctggaacaa taactaaagt
     6661 tgtaaaagat gctttgaata taccagcttt aacaggtatg tatgttgaaa atccaggagc
     6721 agatatgttc aaaaaggatg tttatgtagt agaaacttct gattctgcag caggtatgag
     6781 aaaggcactt cctaagatag ctaaacttgc agtaaaactt gctaatggag aagaaattgg
     6841 aactcctaaa gatgaaggat acatagctag aggaataaga gttaactact tccatgaaga
     6901 cagaggttct aagagagctg ttgacatgtt agttaagaaa ataaaaggtg aaccatttga
     6961 aactgaatac ccaatgccaa actttgatag agttgaccca agtaaagctg taaaagattt
     7021 atcaaaatgt aaaatagctt tagttacttc tggtggtata gttccaaaag gaaatccaga
     7081 tagaatagag tcttcttcag cttctaaata tggaacttat tctatagcag gagttatgga
     7141 tttaacagaa gagacttatg aaactgcaca tggtggatat gacccagtgt atgcaaattt
     7201 agatgcagat agagtattac ctgttgatgt tcttagagat ttagaaaaag aaggtgtaat
     7261 tggaaaatta catgagactt tctatacaac tgtaggtaat ggtacatctg ttgcaaactc
     7321 taaaaaatat gcttctgaaa ttggagcagc attagtggct gatggtgtag atgcagtaat
     7381 attaacttct acatgaggta cttgtactcg ttgcggtgca acgatggtaa aagaaataga
     7441 aaaaactgga cttccagtag ttcatatgtg tacagtagta cctatttcat taacagtagg
     7501 ggctaataga atagttccaa ctatagcaat acctcatcca cttggaaatc cagcgcttga
     7561 ccctacagaa gaaaaagccc taagaagagg ccttgtagaa aaagcattaa atgctttaac
     7621 tacagaagta gatggtcaaa cagtatttga aaaataaaaa taacacacac atatattata
     7681 tagataaaaa tataaacaca cactaaaaat aaaaaataac tatacacaaa aaaaataata
     7741 tagatttcat gaatggataa attggagtta ttcattcatg aaatcttaaa aaatgccaaa
     7801 atacataaac aataatagaa tcaaatatac aataaaatac aatgattaga gcttttaata
     7861 agtttaataa gattaaaaaa taagtgttca taaaatttat ggaggtgcac aaaatgactt
     7921 atccagtttt aaaaggggca ggctatgtac ttattcatac tccagatatg atagtgcaaa
     7981 atggaagtac ttgtacagtt gaaagagcaa caaattcaga ttcagaattt ttaaaagaag
     8041 taagtaatca tataagaagt tatgaagatg tagttaacta catgccaaat caagtttata
     8101 taggaaatag aagaccagaa gaattaagag atttaccaat gccatggtgt gaacaaaaga
     8161 ttgagggaac tagaaatggt aagtttggag aaataatgcc tcaagatgag ttcatagctt
     8221 taatgcaaat aagtgatgca tttgatttag taaaattatc acaagaattt attgatgaag
     8281 taaagccaaa aatcgaaaat aactaccctg aaattgcacc atttgtaggt aaattaaagg
     8341 gtgatgatat agaagaaggt aaagaattag tagcaacaca catagctgaa ggattatacc
     8401 atgatggaaa atttgttggt tatgtaaaaa gagcacatga tgtagatgtt aacttaaatg
     8461 cccatacaat gtttgaaaat ctagtagtaa aagcatctgg agttttatct gctattcaaa
     8521 tgctaagaca tagcaaaata gaccctgctg aaatagatta tgttattgag tgttcagaag
     8581 aagcttgtgg agatataaac caaagaggtg gaggaaactt tgctaaatca atagctgaga
     8641 tagcaggact tcagaatgca actggttctg acacaagagg attttgtgca gcaccaactc
     8701 atgcattaat tcaagcagca gctttagtaa aagctggcat acataaaaat gtaatggtag
     8761 tagcaggtgg agcaagtgct aaacttggaa tgaatgctaa agaccatgtt aaaaaaggtc
     8821 ttcctgtact tgaagatgtt gttggaggat ttgcagtact tgtatctgaa aatgatggtg
     8881 taaatccagt tataagaact gatttaacag gtaagcatac tgttggaact ggttcttcac
     8941 cacaagcagt tatgacagca cttataactt caggtcttga tagagctaat ttaaaaataa
     9001 cagatgttga tgtgtattca gttgaaatgc aaaacccaga tataactaag ccagcaggag
     9061 caggagatgt tccagaagca aactacaaaa tgataggggc acttgctgtt aaacgtggag
     9121 acttagaaaa gaaagaattg aaagattttg tttctaacaa aggattacca ggatgggcac
     9181 caactcaagg acatatacca tcaggagctc cttatatagg atttttaata gatgatttaa
     9241 ctacaggaaa tagaaataga gctatgatag ttggaaaagg tagtttattc ttaggaagaa
     9301 tgactaactt atttgatgga gtatctttta tagctgaaag aaatacaggt gttactgaag
     9361 agacttcagg aatatctaag gatgaaatta agaagataat agctgaatct atgaaaaaac
     9421 tagcattaga tatgttagaa gaataggggt gaggatatgt caaaaaaagt aatcgcagat
     9481 gtattcttag aagtagctaa tgcaatagaa agcggcgaat ttggcaaaaa agtaaaaatt
     9541 ggtgttacta cacttggaag tgaacatggt gtagaaaaca tggttaatgg tgcacaatta
     9601 gcaaaatcta atttatttga tattgtgctt attggaccaa aagtggaaac tgaccttgaa
     9661 gtagttgaag ttaatgatga aaaagaaatg catgcaaaaa tggaagaatt attagattca
     9721 ggatatattg atgcttgtgt tactatgcat tataattttc caataggtgt atctacagta
     9781 ggtagagtaa taactccagc aaaaggaaaa gaaatgatac ttgcaacaac aacaggaaca
     9841 agtgctacta atagaataga ggcaatggta agaaatgcca tttatggtat tgctacagct
     9901 aaatcaatgg gtaacaaatg cccaaaagtt ggaatactta atgttgatgg agcaagacaa
     9961 gttgagaagt gcttaaaaga attaaaagat aatggatatg acatggagtt tgcggattca
    10021 attagagcag atggtggatg tgtaatgaga ggtaatgatt tacttgtagg tgcacctgat
    10081 gttatggtta ctgatacatt atctggaaat atatttatga aagtattttc ttcatatact
    10141 acaggtggag attatgaagc tcaaggcttt ggatacggac ctggagttgg agaagactat
    10201 gatagaaagg tacttatagt atcaagagca tcaggttctc cggtagtagc aaatgcttta
    10261 aaatacgctt atgatgttgt aaagggagat ataagtaatg ttgcaagaaa tgaatttgca
    10321 aaggttaaaa aagcaaaatt tgatgacatt atatcctctc ttactaaaaa agaagttaaa
    10381 gctgaaaaag tagaagttaa aatgccagat aaagaaattg ttactcgtca aatagctggt
    10441 gttgatataa tggatttaga agatgcagta tctgagttat ggaaaaacgg aatctatgct
    10501 gaaagtggta tgggttgtac aggtccaata gttttagtaa atgaagctaa aggagactta
    10561 gcagttgaaa ctttagttaa agctggatat actgcaaaat aagtacattt tttatatgta
    10621 aataagaaca tatagttata attttattga ttataattat atgttcttt
//
LOCUS       DAMJWP010000117        10653 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.122, whole genome shotgun sequence.
ACCESSION   DAMJWP010000117 DAMJWP010000000
VERSION     DAMJWP010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10653
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.122"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(10..654)
                     /locus_tag="PC700_002382"
     CDS             complement(10..654)
                     /locus_tag="PC700_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2581684.1"
                     /translation="MEQHSKVGNFGDLLKVLLDEKELSMGELSKKSGIDKSTISRIAN
                     NKQRPNINHLEKMAIHLNINLEELLKASGYEFRNSNNQIFNADSDFSNFDDILGFANL
                     INDKNFNGNIEKELSKCKLYVQTDEGKKLLFDNFNKKIDSIEKQGQFTDRLRQMYADF
                     CTDGLTIKKYLLIGSMLLYFVISTDVIPDFVFPIGFMDDLVALNIVTKLLKNDK"
     gene            839..1462
                     /gene="ytaF"
                     /locus_tag="PC700_002383"
     CDS             839..1462
                     /gene="ytaF"
                     /locus_tag="PC700_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation membrane protein YtaF"
                     /protein_id="HDF2581685.1"
                     /translation="MIESILLVTSLCIDAFVTSFGYGVNKVKVPLYSNIIISVVCSAI
                     LAISLFAGTIVRDFLPQNITMLICFFILFLLGSARLIESFFKSYFKKKSIHSKNYNFN
                     MLDLELNFNVSIEPDVLDTSKSKVLKSAEAFTLALALSLDGLAIGFGSGLVGVNYIQV
                     ILFSLISNIIAVLLGCLLGNMFVKNVNLNLSWLSGLILLVIGFMKIC"
     gene            complement(1525..3435)
                     /locus_tag="PC700_002384"
     CDS             complement(1525..3435)
                     /locus_tag="PC700_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDF2581686.1"
                     /translation="MNSNTFERLQLNEVKDLIKIHCVSSLGKNLIDKLTPSGNIGVVR
                     RKLAENKEARKIIENSNHIPLEGLFNAGSTIDKIEKGMIIEPVELVNIEDFLRGCRKM
                     KAFMLEKEFYSPTLSSYALNITECKSIEDEINYCIKSNKVDSNASKELKKIRRNIEIT
                     EGKIKDRLNKFITSTVNKKYIQEFIISKRNDRYVIPIKSSYKNEVNGTILDTSSKGNT
                     VFIEPISVSNLSTELTMLKADETIEEYKILSYLTELIFEKISQIKLNIEILSEYDMVF
                     AKAKYSQKIKGITPKINNNGYIKIIKGKHPLLTGDAVPLDFEIGKNYRSLIITGPNAG
                     GKTVTLKTVGLLTLMVQCGFDISAKEGSEFSVFEKVFVDIGDNQSIENALSTFSSHIK
                     NIAEIMSLSNNSTLVLFDEIGSGTEPNEGAGLAISLLEEFYKMGCITIASTHYGEIKK
                     FATLHPEFENAGMMFDKETLEPLYKLTIGKSEDSNALFISKKMGIKNRVLDRAKEYVI
                     DRNYNLDLVKRSKLQSNIEDKNSSEVILSYTDYNVGDKVKLLDEDDFGIVYKPMDKFN
                     NVEVLFNDNFISVNIKRLQLSIKASELYPEGYDLDVLFTSFKERKLEKDIQRGSKKAL
                     KKIQKEIRENRK"
     gene            complement(3972..5039)
                     /locus_tag="PC700_002385"
     CDS             complement(3972..5039)
                     /locus_tag="PC700_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HDF2581687.1"
                     /translation="MSNIKIIASNKCWIEDIARKQLEDISKLNGILRVVGLPDLHTGK
                     IPVGLAVETKNIIYPHIIGNDIGCGMTLFKTGVLKKKFKKDKWIKSLSKIKDLSDIEI
                     KNTYKEECPILNLGTIGSGNHFVEIQCISEIYNKEQFEQLKFSSDDIMMLVHCGSRNY
                     GEDILKKFYDKDGLEVESEKAIDYIKKHDNALMWAERNREAISKKIMQSIGTSEDVET
                     IFSINHNFIEKREDKFIHRKGAVSSERGAVIIPGSRGSLSYIVMPTENTEISLYSLSH
                     GAGRKWSRSVCKSRLKSKYSKDTIKQTKFKSQIICNDVNLLFQEAPEAYKNIEQIIES
                     LIEYELIQVVATMKPLITYKG"
     gene            complement(5285..6280)
                     /locus_tag="PC700_002386"
     CDS             complement(5285..6280)
                     /locus_tag="PC700_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HDF2581688.1"
                     /translation="MYEEVTWLNFIGNRYEVVNSDDVLEINKIYKARDVFYRRNVLIK
                     VIKHNNYICEDFVSNLIDESTALDEINSPYILKIIDVGIHCTEETTLYYVVSEDFNGI
                     GLDELILGNYLHLEAIVNIMIQVLKALEMIHRNHSYHGSLKSSSIIVDTEYNIKICDF
                     GITKANNGVNTRSYGNRRYLCPHQLCINYTDKESDFFATGLILFESIFKKLPFGESNS
                     EEKMLQSIDKGLDWNSIKAINGNTELINVIKRLLGRNNKYTRANDIIIDLSKVMYEKA
                     YIEVEDEKEEEQKQDKVIQVKKEYKSKMRKKLHKKLVVAGLAIVMISMIIISSII"
     gene            complement(6539..7798)
                     /locus_tag="PC700_002387"
     CDS             complement(6539..7798)
                     /locus_tag="PC700_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HDF2581689.1"
                     /translation="MGRRTTRVSRLGKEKKRSVLIVLAALVLIMGGFTYFFNSKFLYN
                     GKIAKNVYIEGVNVSDMTKAEALKAITDKYTPEDLNLTYDGKKYTISPKDIDLKYDTE
                     DVVKDAYESTKKGSYFQNLKKYIDIRVNKANMKIKAEYDEAKLSSKVSSIADSINVKM
                     KNASISVGSGGLSYTDSVVGREFDLAANKESIYNMIKNKEHKTLELKVNLQKPDITTE
                     QVKTVNSVIGQYSTTYSQAVEGRSYNVGLSARKTSDVLLMPGEEFSYNKLTGPSNKAN
                     GYKDAPVIVYGKLEQSAGGGVCQTSSTVYNAALLSGMEITQVTNHSSASTYVPKGRDA
                     TVSDGGLNLKFKNPYKHPVYIKNYAGGGSVSSVIYGNSGDKPNISIEVKQTGQNKYST
                     YRIFKDSSGKVIKKEHISNSSYKELKK"
     gene            complement(8037..8666)
                     /locus_tag="PC700_002388"
     CDS             complement(8037..8666)
                     /locus_tag="PC700_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDF2581690.1"
                     /translation="MKIVTYNIHKGMDSNNRLTLTKMGLYLKQLDCDVICLQEVLYPQ
                     FLALKAVLNMDGVFATNVKKVNMIYGICTFTTSKMLNSNHFFLTSKKEQRGALCITID
                     AYGRIINVINTHLGLDRQERAKQLDEIIDYRNRLVGIVILCGDFNEKNVFLSMFNDMA
                     ISLNKSYLPTFEKSNSRIDYIFVNKNTELKGYTVEKIYLSDHYPVIGYI"
     gene            complement(8859..9020)
                     /locus_tag="PC700_002389"
     CDS             complement(8859..9020)
                     /locus_tag="PC700_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581691.1"
                     /translation="MIIPSRRIQDITLTNLKNGEVTLIELDEIYKKMGFLFVVSEGKL
                     KKIKKENHH"
     gene            complement(9363..10523)
                     /locus_tag="PC700_002390"
     CDS             complement(9363..10523)
                     /locus_tag="PC700_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HDF2581692.1"
                     /translation="MIETGFVYCSFLVVFVGVIFFMVDKLKWKVFDVINPMLLIYLGS
                     AILGSFGLFAQNEDVGTYQNIITVNFLPLMLVFLLIQCDLRKILKMGPKMLLSFFCAT
                     FTFMLGFVIAFIIFKNSMGADSWKILGAASGSWIGGSSNMIAAANALGVAEEGLSYAI
                     LMGSIGYTVWMSVCLMSVKFGPKFNKWTKCDTSFIDEVTSKLEKENKDNTAPTFVELM
                     ILLSVGFGVASFAQIVSKILPTSGILNETMWVVLVASTVSILLGMTKISKLKGSTLVG
                     NVFMYLLLAATGSKANFFGLSEAPVYIMFGLVVVLVHAISFIGLAKLFKLDLFTCEVG
                     SIANIGGVTTAPMIAGVHNPVLIPVGVLMGLLGNIIGTYCALVVTQILHMLA"
BASE COUNT         3489 a         1748 c         1052 g         4364 t
ORIGIN      
        1 ttctaaaact tacttatcat ttttaagtaa ttttgtaact atatttaatg ctactaaatc
       61 atccataaat cctattggga acacgaagtc tggaatcaca tctgttgata taacaaagta
      121 aagtagcata cttccaatca ataaatattt ctttattgta agtccatctg tacaaaaatc
      181 agcatacatt tgtcttaatc tatctgtaaa ttgaccttgt ttttcaatag aatctatttt
      241 tttattaaaa ttatcaaaaa gtaatttttt accttcatct gtttggacat ataacttaca
      301 cttacttaac tctttttcaa tatttccatt aaagttctta tcattgatta aatttgcaaa
      361 tccaagaata tcatcaaagt tactaaaatc actatctgca ttaaatattt gattattgct
      421 gtttctaaat tcatatcctg aagctttcaa cagttcttct aggttaatat ttaaatgtat
      481 tgccatcttt tctaaatgat ttatatttgg cctttgttta ttattagcta tacgagatat
      541 agtagattta tcaattccac tttttttact tagttctccc attgataatt ctttttcatc
      601 taataaaact ttcaacaaat caccgaaatt cccaacctta ctatgttgtt ccattatttt
      661 cacctgcttt attatgattg gttttatatt atatgatatg attcttttgt tatttaggtg
      721 ttgtatgatg ttgaaatttt atcaacaatt tattggatta tctgtaacaa tttatccatt
      781 tttacaatac tataactaat ataagtacaa aatttaagag aaaggatgtt aatctactgt
      841 gattgaatca attttattag ttacttctct ttgtatagat gcctttgtga caagctttgg
      901 atatggagta aataaagtta aagttccctt atattcaaat ataattatca gcgtagtttg
      961 ttcagctatt ttagcaatat cgctttttgc aggtacaata gttagagatt ttttacctca
     1021 aaatataaca atgttaatat gtttctttat tttattttta ttaggaagtg ctagactaat
     1081 tgaaagtttt tttaaatctt attttaagaa aaaatctata cactcaaaga attataattt
     1141 taatatgtta gaccttgaat taaattttaa tgtaagtatc gaacctgatg tcttagatac
     1201 aagtaaatct aaagtgctta aatctgctga agcatttaca cttgcacttg ctttaagttt
     1261 agatggatta gctatcggtt ttggtagtgg ccttgtaggt gtgaattata tacaagttat
     1321 tctattttct ttaatatcaa atataattgc agtattactt ggttgtttac taggaaatat
     1381 gtttgttaaa aacgtaaatt taaacttatc ttggttaagt ggtttaatct tgttagtcat
     1441 tggatttatg aagatatgtt aataagaaag caatctaaag ataaactttt ctctttagat
     1501 tgctttcttt tgtgcttttt atatttattt tctattctct ctaatttctt tctgaatctt
     1561 tttaagagct ttttttgaac ctctttgaat atctttttct aattttcttt ctttaaaact
     1621 agtaaataat acatctaaat catacccttc tggatacaat tctgatgctt ttatgctaag
     1681 ttgtaatctt tttatattta ctgaaataaa attatcatta aacaatactt ctacattatt
     1741 aaacttgtcc ataggcttat atacaattcc aaaatcatct tcatctagta attttacctt
     1801 atcccctaca ttataatctg tatagcttaa aatgacttca gaactatttt tatcttcaat
     1861 attagattgt agcttacttc ttttaactaa atctaaattg tagtttctat caattacata
     1921 ttctttagct ctatctaaaa ctctattttt tattcccatt tttttagaaa taaaaagagc
     1981 attactatct tcagattttc caatagttaa cttatacaat ggttctaacg tttctttatc
     2041 aaacatcata ccagcatttt caaattctgg atgtaatgta gcaaactttt ttatctctcc
     2101 ataatgagtt gatgctatag ttatacatcc cattttgtaa aactcttcta ataatgatat
     2161 tgctaaacca gctccttcat taggttcagt tccacttcct atttcatcaa acagaacaag
     2221 agtagaatta tttgataaac tcataatctc tgcaatattt tttatatgtg atgaaaaagt
     2281 acttaaggca ttttcaatac tttgattatc ccctatatct acaaaaactt tttcaaatac
     2341 actaaattca ctaccttctt ttgcagaaat atcaaatcca cattgaacca ttaaagttag
     2401 taaaccaaca gtcttaaggg ttacagtttt tcctcctgca ttaggacctg ttattataag
     2461 acttctataa tttttgccta tttcaaaatc taaaggtact gcatctcctg ttagaagtgg
     2521 atgtttacct ttaattattt ttatatagcc attattattt atttttggag taattccttt
     2581 tattttttga ctatacttag cttttgcaaa taccatatcg tattctgaaa gtatctctat
     2641 atttaatttt atctgactta tcttctcaaa tataagttct gttaaataag ataatatttt
     2701 gtattcttca atagtttcat cagcttttag catagttaac tctgtactta aatttgaaac
     2761 actaataggt tcaataaata cagtatttcc ttttgatgat gtatctaata tagtaccatt
     2821 aacttcattt ttataagaag atttaattgg tattacatat ctatcatttc ttttacttat
     2881 tataaactct tgtatatact ttttatttac agttgatgtt ataaatttat ttaatctatc
     2941 ttttatcttt ccttctgtaa tttctatatt tcgtcttatc ttttttaatt ctttactagc
     3001 attagaatca actttattag attttataca gtaatttatc tcatcttcta tacttttaca
     3061 ttctgttata tttaaagcat aagaacttaa tgtaggtgaa taaaattctt tttctaacat
     3121 aaaagctttc atcttcctac aacctcttaa aaaatcttca atatttacaa gttcaacagg
     3181 ttctattatc attccttttt caattttatc tattgtagaa cctgcattaa acaatccttc
     3241 aagtggtatg tgattactat tttcaattat ctttctagct tctttatttt ctgctaattt
     3301 ccttcttact actccaatat tcccactagg agttaattta tctattaaat tttttcctag
     3361 cgaacttaca caatgtattt ttattaagtc tttaacttca tttagttgca atctttcaaa
     3421 tgtattacta ttcattattt tttctccttt atttataagt catcgtcttt taaatcaccc
     3481 tatcatttac tttgataata gttattttgc gaacaactta ataaataagg agttattata
     3541 tatcaaactc cttatttatt aagaagttta actccggact cattaatgtt agtatttttt
     3601 ttcataaaac acttctcctt ttctattctt atttaaacac ttataccagt gcttctaata
     3661 ttattccata aattatatat ttatgtcaat aaaaataatg ttattaatgt ttttgtacag
     3721 tgaaaatttt tatagtgaaa ataagcttat actttcattt atattttgga attacttata
     3781 ttgtcatcaa ttcatgtatg tgtagtttcc aatacaatct aactttgtaa tcattatata
     3841 tcaaagaatt tagctctata aattatcgtc tctataaaat aaaaaaatag ctaataataa
     3901 taaaacactt ttaaatgttc cataaaatta gctatttata atataatttt aatctttttt
     3961 aactttaaaa attatccttt ataagtaatc aatggcttca tagttgccac tacttgaata
     4021 agttcatatt ctatcaaact ttcaataatc tgctctatat tcttatatgc ctctggagct
     4081 tcttgaaata ataaatttac atcattacat ataatctgac ttttaaattt agtttgtttt
     4141 atagtatctt tactatactt acttttaagg cgcgatttac aaactgaacg agaccatttt
     4201 ctacctgctc catgtgatag agaatataaa gatatctctg tattttcagt aggcataact
     4261 atatatgata gactgcctct tgaacctggt ataatcacag ctcctctttc acttgaaaca
     4321 gcaccttttc tatgtataaa tttatcttcc cttttctcta taaagttatg atttatagag
     4381 aatattgttt caacatcttc agatgtacct attgactgca taattttttt actgatagct
     4441 tcacgattac gctctgccca cattaaagca ttatcatgtt ttttaatata atctattgct
     4501 ttctcacttt caacttcaag tccatcttta tcataaaatt tttttaaaat atcttctcca
     4561 taatttcttg acccacagtg aactaacatc ataatatcat cagatgaaaa ttttaattgt
     4621 tcaaattgtt ctttattata aatttcactt atacattgaa tttctacaaa atgatttcca
     4681 ctccctatag ttcctaaatt taaaattgga cattcttctt tatatgtgtt ttttatttct
     4741 atatcggaca aatctttaat cttactaagt gacttaatcc atttgtcttt tttaaatttc
     4801 ttttttagaa ccccagtttt aaataaagtc attccgcatc ctatatcatt tccaattata
     4861 tgtggataaa taatattttt agtctcaact gctaaaccta caggtatttt acctgtatgt
     4921 aagtcaggta atcctactac tcttaaaatt ccatttaact ttgaaatatc ttctagttgt
     4981 ttccttgcta tatcttcaat ccaacattta ttacttgcta ttatttttat attgctcata
     5041 atttgctcct ttttaatatt ctcctgcctt taaaaagtag caaaaagctg tgacctttag
     5101 ccacagcttt tttattctaa actgtaaaag caggtggcta tttgttaaaa tactcctaga
     5161 cgtttaatac ctcattattt tttgttaaca taataaccac ttcctttcat aatttaagac
     5221 tacactaaaa tttctatgta tatcatttat agacattgat tttaaaatac ttaatataca
     5281 atttttatat tatggaacta ataataatca ttgaaatcat gactatagct agaccggcaa
     5341 caacaagttt tttatgtaat ttttttctca ttttactttt atattctttt ttaacttgta
     5401 taactttgtc ttgtttctgt tcttcttctt tctcatcttc aacttctata tatgcttttt
     5461 catacattac tttacttaaa tcaataataa tatcattggc tctagtatac ttattatttc
     5521 ttccaagaag cctttttata acatttataa gttctgtatt tccattaatt gcttttatac
     5581 tattccaatc taatccctta tcaatagatt gtagcatttt ttcttctgaa tttgattctc
     5641 caaaaggcaa ttttttaaat atagattcaa atagaataag acctgttgca aaaaaatcac
     5701 tttctttatc agtataattt atacatagtt gatgtgggca taaatatctc ctgtttccat
     5761 agcttcttgt attaacccca ttatttgctt ttgtaattcc aaaatcacaa atttttatat
     5821 tgtattctgt atctactatt atactactcg attttaaact accatgatat gaatgattcc
     5881 tatgtatcat ttctaaagct tttaaaactt gtatcattat gtttacaatt gcttctaaat
     5941 gaagataatt acctagaatt aattcatcta gtccgatacc attaaagtct tcacttacaa
     6001 cataatataa tgttgtttct tctgtacagt gaatcccaac atctattatt tttagtatat
     6061 aaggagagtt tatctcatct aaagcagtac tttcatcaat taaatttgaa acaaaatctt
     6121 cacaaatata attattgtgc tttataactt ttattaacac atttctcctg taaaaaacat
     6181 ctctagcttt atagattttg ttaatttcta aaacgtcatc agaatttact acctcatatc
     6241 tattgcctat aaaattcaac catgtcacct cctcatacat tttatataac taaatctatt
     6301 tacaattata ccaatatata ggaatatttt ctatatatta atgattgtaa tattaaaaca
     6361 agaactaata gtgttaattt ttacaaaaaa ggctgtctca aaatagagat acgcttcttt
     6421 tgtattgttg aaatgatgaa aatatatttg atttcacata aaaagagtgt ctcataaact
     6481 aaaaagttca ttttgagaca ctccctttta tattacaaat atttctctaa aaaattatct
     6541 atttttttaa ttctttataa gaactatttg atatatgttc tttttttatg acctttccac
     6601 ttgaatcttt aaatattcga tatgtagaat atttattctg accagtttgt tttacctcta
     6661 tagatatatt tggtttgtca ccagaattac catagattac tgatgataca cttcctccac
     6721 ctgcataatt tttaatatat acaggatgct tatatggatt tttaaacttt aagtttaatc
     6781 ctccatcgct cacagtagca tctcttcctt taggcacata agtagatgca gatgaatgat
     6841 ttgtaacttg agttatctcc atacccgaaa gaagagccgc attataaact gttgaagatg
     6901 tttgacaaac acctcctcct gctgattgtt ctagcttacc ataaactata acaggtgcat
     6961 ctttgtaacc atttgcttta ttactaggtc ctgtaagttt attgtatgaa aattcttcac
     7021 ctggcattaa taatacatca ctagtttttc ttgctgataa tcctacatta tatgacctac
     7081 cctctacagc ttgtgaataa gtagttgaat attgacctat aacagaattt acagttttta
     7141 cttgttcagt tgttatatct ggtttttgaa gatttacttt aagttctaaa gttttatgct
     7201 ctttattttt tatcatatta tatatagact ctttatttgc tgccaaatca aattctctac
     7261 ctacaacaga gtctgtataa cttagtccac cacttccaac actaatagaa gcatttttca
     7321 tcttaacatt tatactatct gctattgaag acactttgga actaagtttt gcctcatcat
     7381 attcagcttt tatcttcata tttgccttat tcactcttat atcaatatat ttttttaagt
     7441 tttgaaaata ggaccctttt tttgtacttt cataagcatc ttttacaaca tcttctgtat
     7501 catattttaa atcaatatct tttggtgata ttgtatattt tttcccatca taagtaagat
     7561 ttaagtcttc tggagtatac ttatctgtta tagcttttaa tgcctctgct ttagtcatat
     7621 cagatacatt tactccttct atatatacat ttttagcgat tttcccattg tacagaaatt
     7681 tactgttaaa aaaataagta aatccaccca ttattagaac taaagctgcc aatacaatta
     7741 aaacacttcg ttttttttct ttacctaatc ttgaaactct tgttgttctt ctgcccataa
     7801 cttcacctca ttttgacact agcttaaatt tgcataatag agttcccatt tcaaaactac
     7861 cttagattat atttgaatag cttttgttaa acaaggaatt taatcaaatg taactttatt
     7921 gtaaatttat gtcttataat tagttttatt tttgatattt tttattatat ttcttttact
     7981 atttcttaac aaaaaattgt ttattttata acaactaatt tcatttatat tgaaatttaa
     8041 atgtaaccta ttacaggata atggtctgac aggtaaattt tttctactgt gtatcctttt
     8101 aattcagtat ttttattaac aaaaatgtaa tctattctag agtttgattt ttcaaatgtt
     8161 ggtaagtaag acttgtttag tgaaatagcc atatcattaa acatacttaa aaagacattt
     8221 ttctcattaa aatcaccaca taaaataact atccccacta atctgttcct atagtctatt
     8281 atttcgtcta attgttttgc tctctcctgc ctatcaagtc ccaagtgagt atttattaca
     8341 tttataattc ttccatatgc atctatagtt atacaaagag ctccccgttg ttcttttttg
     8401 ctagttaaga aaaaatgatt actgtttaac attttagatg ttgtaaatgt acaaatacca
     8461 tatatcatat ttactttttt tacatttgta gcaaagacac catccatatt taaaacagct
     8521 tttaaagcta aaaattgagg atataatact tcttgcaaac aaattacatc acaatccaat
     8581 tgttttaaat ataaccccat tttagttaga gttaatctat tatttgaatc cataccttta
     8641 tgaatattgt aggttactat tttcataata atatcacctc ttatgcaata taatacttac
     8701 taaaatttaa acttatttac ttttcactta agaccattat ttatcttata aaattgagta
     8761 cttataaact ttatcacaaa aaaacctaac ttaagtattt atattctaca taagttaggt
     8821 tttataaact attataatat ttattatttc attcatttct aatgatgatt ctcctttttt
     8881 atctttttca atttgccttc actaacaaca aataaaaatc ccatcttctt gtatatttca
     8941 tccaattcaa ttaaagttac ttcaccattt tttaaatttg ttaatgttat atcttgtatt
     9001 cttcttgatg gaattatcat aagttatcaa cactccttta aattcttttt aatctttata
     9061 tttaaatata tttatgcatt ctgatatata ttcataaatt attaaaataa attcaaaaaa
     9121 cacccgttct gcaaacattc gttctgtata tttattatac atcatacaga cgttcgattc
     9181 aatataaaaa ttaaatatat ctcgttttta gtaaatttta caatattata cactaaatat
     9241 gtcaaaacat cttcttgaaa tagattatct tgtcaaacaa aaaaaaccat ttcagtaaaa
     9301 gcttctgctt aaattgaaat gacttttctt atttaaatta ttaattttat accatatttt
     9361 tattaagcta acatatgtaa tatttgagtt accacaagag cacaataagt ccctataata
     9421 tttcctagaa gccccataag tacacctact ggtattaata caggattgtg aaccccagca
     9481 atcattggag ctgtagttac tcctcctata ttagctatac ttcctacttc acaagtaaat
     9541 aaatcaagtt taaatagttt agcaagccct ataaatgaaa tagcatgtac aaggactact
     9601 accaatccaa acattatgta aacaggtgct tctgacaatc caaagaaatt tgctttacta
     9661 cctgtagctg caaggagtaa atacataaac acatttccta ccagtgtact ccctttaagc
     9721 tttgatattt ttgtcatacc caataatatt gatactgtag atgcaacaag tactacccac
     9781 atagtttcat taagtatacc tgaagttgga agtatcttgg atacaatttg tgcaaaagac
     9841 gcaactccaa acccaacaga aagtaaaatc ataagttcta caaacgtagg tgccgtatta
     9901 tctttatttt ctttttcaag ttttgatgta acctcatcta taaaacttgt atcgcacttt
     9961 gtccatttgt taaatttagg tccaaatttc acagacatta gacaaacaga catccatact
    10021 gtatatccta tagaacccat aagtattgca taactaagtc cttcttctgc aactcctaga
    10081 gcattagctg ctgcaatcat atttgatgag ccacctatcc atgaaccaga tgctgcccca
    10141 agaattttcc atgaatctgc acccattgaa tttttaaaaa ttatgaatgc tataacaaat
    10201 ccaagcataa atgtaaatgt agcacagaaa aatgataata acattttagg ccccatcttt
    10261 aatatttttc ttaaatcaca ttgaattaac aagaacacta acattaatgg aaggaagttc
    10321 actgttatta tgttttgata cgttccaaca tcttcattct gtgcaaataa accaaatgat
    10381 ccaagtatag ctgaacctaa gtatattaaa agcattggat taataacatc aaataccttc
    10441 catttcaatt tatcaaccat aaaaaaaatt acacctacga atacaaccaa gaaactacaa
    10501 tatacaaatc cagtctcaat cacattatac acaccctttc ttttcctaat taatttgatt
    10561 tttatgattt ctaagtaaag actttttcag ataatagaca attaaaataa atacttttat
    10621 ctatatcatg ctaattaaat acatatatac cta
//
LOCUS       DAMJWP010000118        10605 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.123, whole genome shotgun sequence.
ACCESSION   DAMJWP010000118 DAMJWP010000000
VERSION     DAMJWP010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10605
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.123"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            288..746
                     /locus_tag="PC700_002391"
     CDS             288..746
                     /locus_tag="PC700_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2581693.1"
                     /translation="MEFINFVSDKVIDLKMDCKTKEDFFKEIHNKVFDLGFVKEEFGE
                     KILARENVFPTGLNLGDYGVAIPHTDAEYIKEQFISVCTFNEPVVFSSMEDQDEKVPV
                     NLAFILGLNQPHSQLSVLTELMGIMQNKELVDKLMSSTDKEEVLRTLKSL"
     gene            807..1082
                     /locus_tag="PC700_002392"
     CDS             807..1082
                     /locus_tag="PC700_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2581694.1"
                     /translation="MKKILVACGAGIATSTVVCDKVERLVKENNIQAEVIQCKIAECA
                     TKQEGADLIVSTTILPTTYNIPAIKATAYITGVNTAALDKKILDALK"
     gene            1141..2415
                     /locus_tag="PC700_002393"
     CDS             1141..2415
                     /locus_tag="PC700_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012951111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDF2581695.1"
                     /translation="MDKLLAVVQYILNMGPTVILPITIVVLGMIFRIKFTKALKSGLT
                     IGIGFVGINLVVNLLTSTLGPAAQGMVERFGLNLTVIDVGWPTTASATWASPVAPILI
                     PVCLLVNLLLLYFNKTKILNVDIWNYWHFIVAGAVGYIVTGSYVWAIFCAILMELLVL
                     FIAEKTAKNVQEFYDLEGIALPTGSTASFAPLGYLVGKFVEKIPGINKIQADPDSIQK
                     RFGIFGEPMMMGIILGIILGALAGYDVAGIFNVGISMGAVMLLMPRMVRILMEGLIPI
                     SESIRDFLQAKYGDRELYIGLDAAVATGHPAVISTALILVPITLFLAVILPGNKVLPF
                     GDLATIPFYMAFIVCFRKGNIVQSVITGTIVIAISLYMATNFADVHTQLLQQANMMPE
                     GTTMVTSIDTGGNLLKWGILKLSQLVSSFGIF"
     gene            2545..3597
                     /locus_tag="PC700_002394"
     CDS             2545..3597
                     /locus_tag="PC700_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF2581696.1"
                     /translation="MKAAVLHGTNDMRFEDIEIKPCESDEVKIKVMAAGICGSDPPRV
                     LKHWKYPVPAIPGHEFSGVIAEVGKDVKNVKVGDRVVAIPFIPCNECEYCKRGLFSLC
                     DDHGMLGAKSFGAFAEYVNIKATNVLPIGDMDFEDAAMIEPLAVAMHGVLNIGVQVGD
                     AVAVMGSGTMGQLVIQGLKIAGAGTIIAVDISDNKLRESKELGADIIINAKDIDPVEK
                     IKELTGGKGVDIALECAGSKITQEQCLLITKKKSKIGFLGIAYSDITLSEEAFENIFR
                     KELELKGFWNSYSAPFPGQEWTKGINLVNEGKIKLKEMVSHRFSLEDTYKAFEMIRDR
                     KEEFNKILILPQGVEK"
     gene            3597..4628
                     /locus_tag="PC700_002395"
     CDS             3597..4628
                     /locus_tag="PC700_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDF2581697.1"
                     /translation="MKAVVKTKPGYDNVEVLEVEEPKATGDKVKIKVEYSGICGSDIH
                     SFKGEYANIKAPVTLGHEFSGIVVEVGEDVKDIKVGDRVTSETTFATCEKCIYCETKD
                     YNLCPTRKGIGTQVNGSFAEYVLSREESIHVLPENVSLLSAALTEPLACCVHAALEKT
                     TIESSDTVLIIGPGPIGLLLAQVVKSQGATVIMSGVTKDKERLEIAKNLGVDRTVNVM
                     EESLADVVNEMTDGNGVNKGFDCSGFMPAVNEALRLLRKKGTFVQVGIFAKKLNEMDQ
                     EAIIQRELQYIGSRSQKPSSWITALDLMASGKVNTEALVTRTVGLDEFKEGITALMNG
                     EEIKVAVKS"
     gene            4720..5532
                     /locus_tag="PC700_002396"
     CDS             4720..5532
                     /locus_tag="PC700_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDF2581698.1"
                     /translation="MSTLRDHSNNIRKNIIKMVAEAKSGHPGGSLSIADMLTVLYFDK
                     MNVCAENPKMADRDRFVLSKGHAAPALYATLAEKGFFPEEELITLRKFGSKLQGHPDM
                     KKVAGIDMSTGSLGQGLSAANGMALAGKLDNKDYTVYTILGDGEIQEGQIWEAAMTAS
                     HYKLDNLIAFVDLNGLQIDGSNEEVMNVNPVDDKFKSFGWNVIVINGHSFEEIGNAVD
                     EAKKVTGKPTVIIAKTVKGKGVSFMENNAAWHGTAPNAEQAEQALKDLEGGL"
     gene            5535..6455
                     /locus_tag="PC700_002397"
     CDS             5535..6455
                     /locus_tag="PC700_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HDF2581699.1"
                     /translation="MGIATREAYGQVLKELAENKDIVVLDADLGKATKSISFKEVAPD
                     RFFDMGIAEGDMIGTAAGLATCGKIPFASTFAIFAAGRGYEQIRNSVAYPNLNVKIAA
                     THAGVTVGEDGGSHQAIEDISLMRGIPNMVVLNPADALEARQAILASIDYNGPVYIRL
                     GRAATPDVNSENYKFEIGKGTVLREGSDITVIATGIMVAKALEAAEELAKEGINVEVV
                     NISTIKPLDEALIKETAKKTGKVVTAEEHSIIGGLGSAVCEALAETKDVVVRRIGVKD
                     VFGQSGTPADLLKHYGLTTEDIVKNIKELL"
     gene            6630..7076
                     /gene="rpiB"
                     /locus_tag="PC700_002398"
     CDS             6630..7076
                     /gene="rpiB"
                     /locus_tag="PC700_002398"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HDF2581700.1"
                     /translation="MKISIGCDHGGYELKEYIKENLINKGIEVEDVGTNSKESVDFPI
                     YAKKVSQNVQSKKSDLGILCCGTGIGMSIAANKFKGIRAAVVSDCFSAKATREHNNTN
                     VLCLGERVIGKGLAMRIVEEWLNSSYNGERHARRLQMIDDIEKENL"
     gene            7115..7780
                     /locus_tag="PC700_002399"
     CDS             7115..7780
                     /locus_tag="PC700_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDF2581701.1"
                     /translation="MSIICASIMCADQMKLKEELEALEEAGVKLLHCDVMDGIFVKNL
                     AMGPELLKSISENTTIPLDIHLATETPDKYIDMMSYIKPKYISFHVESSTNVKADIQK
                     LRNYGIGPVLAISPQTSVDKIEEYISLVEGILVMTVNPGFAGQKFNLSVLDKLDKLTE
                     ILKDYDNPPFIEVDGNINKDTIKLMNGKKVDIYVVGTSALFNDKPPISYKDKIEELKE
                     SIK"
     gene            7890..9392
                     /locus_tag="PC700_002400"
     CDS             7890..9392
                     /locus_tag="PC700_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HDF2581702.1"
                     /translation="MGDLYKLQNGTDIRGIAYENDSKEVNLTVEEVKKIAKAFHIWLK
                     EKTKKDKVTVAIGTDSRITGSQFRSTVIETLTNDDCNVIDCEIATTPAMFMTTIMDGY
                     NSDGAIMITASHLPYYYNGLKFFTESGGLEKTNIKEMLDIAVKNDSVDYEEVNKKGEV
                     IVKNLIEDYSNLLIDKIRKGVNSSKNYEKPFSGLKILVDAGNGAGGFFAEKVLHILGA
                     DTTGSQFLNPDGMFPNHIPNPENKEAMESICKAVLDNNSNLGIIFDTDVDRAAIVGKN
                     GKPINKNALIAVISSIVLEEHPKTAIVTDSITSEGLAKFINELQGRHHRFKRGYKNVI
                     NEAIRLNSEGEECHLAIETSGHAALKENYFLDDGAYLIAKILIKVAKLSLEGKTIEEL
                     IENLEEAQEEKEVRIGINKEDFRPYAEDILKDLDSHVKSIDGWSVAPNNFEGIRVNCD
                     ESNGDGWFLLRISLHEPLLALNIESNKIGGAEQIYSKLKLFLEKYDLKGL"
     gene            complement(9704..>10605)
                     /locus_tag="PC700_002401"
     CDS             complement(9704..>10605)
                     /locus_tag="PC700_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2581703.1"
                     /translation="LFTLVNTVFHFYIYNFLSVKFNYINNISKNIKNIENGNLKYKLE
                     VIGDDEISTLAKSINNITGGLETAVDANIKNERMKAELITNVSHDLKTPLTSILSYVD
                     MLKNNDFDRETINDYINILDKKSQRLKLLIDDIFEASKLSSGDVEFNITKTDIRELLI
                     QSIVEFEDKIQNSSLDFIIETPEYAVFTMIDGKKTWRVFENLICNILKYSMPNTRVYI
                     DMFIKEENIILTFKNISNDKLNLKPEELIERFRRGDISRKTDGSGLGLSIAQNIIELE
                     NAHMEIIIDADLFKVMLTFRRVN"
BASE COUNT         4234 a         1210 c         1956 g         3205 t
ORIGIN      
        1 acataaaaaa ccatttgtaa aaaacaaaaa aatcatttct ctaacaaaaa aaaccacaaa
       61 aaatataaaa aagtatggct agttaagtga ttaacaagta aaaattaaaa taaaaatcaa
      121 aaaaaattta tcaatgaaag tcagctaaaa tcctggaaga gagcattatt ttcgggaaaa
      181 cattttcttt tgtttcacaa aaaaagccac tttttggcac gcaacatgct ttatataaag
      241 tcaagaaaaa ctaaggtata agacaaaggc aattaggagg tgtaaatatg gagtttataa
      301 attttgtaag tgataaagtt atagacctta aaatggattg taaaacgaaa gaagatttct
      361 ttaaagaaat acataataaa gtttttgatt taggctttgt aaaagaagaa tttggagaaa
      421 aaatattagc aagagaaaat gtatttccaa caggtttaaa tttaggtgat tatggagtag
      481 caataccaca tacagatgca gaatatatta aagagcaatt tatatcagtc tgtactttta
      541 atgaaccagt agtatttagt tctatggaag accaagatga aaaagtacct gtaaatttgg
      601 ctttcatatt aggacttaat caaccacaca gtcaattatc tgtattaaca gaattaatgg
      661 gaattatgca aaataaagag ttagttgata agttgatgag ttctactgat aaagaagaag
      721 ttttaaggac actaaagtct ttataaaaat aaaaaagtac taaaaataaa aaatataaaa
      781 ataaaaatta ttaggaggaa ataaaaatga aaaaaatatt agtagcatgt ggagcaggaa
      841 tagcaacatc aacagtagtt tgtgacaagg tagagagatt agttaaggaa aataatatac
      901 aagcagaagt tatacaatgc aaaatagctg aatgtgcaac aaagcaagaa ggagcagatt
      961 taatagtatc aacaactata ctaccaacaa cttataatat accagcaata aaagcaacag
     1021 catatataac tggagtaaat acagcagcat tagataaaaa aatattagat gctttaaaat
     1081 aatcctatca tagatacatt gaaataatat tttataaaac agaaaaagga ggatttataa
     1141 atggataaat tattagcagt agtacaatac attttaaata tgggaccgac agtaatacta
     1201 ccaataacaa ttgtagtact aggaatgata tttagaatta agtttacaaa agctttaaag
     1261 tcaggtctta caattggtat aggatttgtg ggtataaact tagttgtaaa cctattaaca
     1321 tcaacactag ggccagcggc tcaaggaatg gttgaaagat tcggacttaa tttaacagta
     1381 atagatgttg gttggccaac aacagcatca gcaacatggg cttcaccagt tgcaccaata
     1441 ttaataccag tttgtttatt agtaaactta ctattattat acttcaataa aacaaagata
     1501 ttaaacgttg atatatggaa ttattggcac tttatagtag caggtgcagt tggatatata
     1561 gtaacaggaa gttatgtttg ggctatattc tgtgcaatac taatggagtt attagtactg
     1621 tttattgcag aaaagacagc aaaaaatgtt caggagtttt atgatttaga aggtatagca
     1681 ttaccaactg gttcaacagc ttcatttgct ccacttggat atttagtagg taaatttgtt
     1741 gaaaaaatac caggaataaa taaaatacaa gcagacccag actctataca aaaaagattt
     1801 ggtatatttg gagaaccaat gatgatgggg attatactag gtataatact tggagcactt
     1861 gcgggatatg atgtagcagg aatattcaat gtaggtatat caatgggtgc agtaatgtta
     1921 cttatgccta gaatggttag aatattaatg gaaggtttaa ttccaatttc agaatcaata
     1981 agagatttct tacaagcaaa atatggagac agagaacttt atataggtct ggatgcagca
     2041 gtagcaacgg gacatccagc agttatatca acagctctta tacttgttcc tataacgttg
     2101 ttcttagcag ttatactacc aggaaacaaa gttttaccat ttggagactt agcgacaata
     2161 ccattttata tggcatttat agtgtgcttt agaaaaggta atatagttca atcagttata
     2221 acaggaacaa tagtaatagc tatatcactt tatatggcta caaactttgc agatgttcat
     2281 acacaattat tacaacaagc aaatatgatg ccagaaggaa caacaatggt tacaagtata
     2341 gatacaggtg gaaacttgct aaaatgggga atattgaaat tatctcaatt agtttcttca
     2401 tttggaatat tctaatatgt agttaataga gattactctt ttttagtaaa gtatgcgaaa
     2461 atttaacatt tagcagctac taaaaaagag taatattttt tataaatttt taaaaataca
     2521 aaatacaaga ggaggagatt tatcgtgaaa gcagcagtat tacatggcac aaatgatatg
     2581 agatttgaag atatagaaat aaaaccatgt gaaagtgatg aagtaaaaat aaaagttatg
     2641 gcagcaggaa tatgtgggtc agatcctcca agagtattaa aacattggaa atatccagtt
     2701 ccagctatac caggacatga gttttctgga gtaattgctg aagttggtaa ggatgtaaaa
     2761 aatgtaaaag ttggagatag agtagttgca attcctttta taccttgtaa tgaatgtgaa
     2821 tattgtaaaa gaggattatt ttcattgtgt gatgaccatg gaatgttagg agctaaaagt
     2881 tttggagctt ttgcagagta tgtaaatata aaagctacaa atgtacttcc aataggagat
     2941 atggactttg aagatgctgc aatgatagag ccactagcag ttgctatgca tggagtactt
     3001 aatataggtg ttcaagttgg agacgctgta gccgtaatgg gtagtggaac aatggggcag
     3061 ttggtaatac aaggacttaa aatagcagga gcaggaacaa ttatagcagt tgatatatct
     3121 gataataaat taagagaatc aaaggaatta ggggctgata taataatcaa tgctaaagat
     3181 attgacccag tagaaaaaat aaaagaactt actggtggaa aaggcgttga catagctctt
     3241 gaatgtgcag gttcaaaaat aactcaagaa caatgtttac ttataactaa aaagaaaagt
     3301 aagataggat ttttagggat agcatattca gatataactt tatcagaaga agcatttgaa
     3361 aatatattta gaaaagaact agaattaaaa ggtttttgga actcttattc tgcaccattt
     3421 ccaggacaag aatggactaa gggaatcaat ctagttaatg aaggaaagat aaaattaaaa
     3481 gaaatggtat ctcatagatt ctcacttgaa gatacttaca aagcatttga aatgataaga
     3541 gatagaaaag aagaatttaa caaaatatta attttaccac aaggggttga aaaataatga
     3601 aagcagtagt taaaactaaa ccaggctatg ataatgtaga agttttagaa gtagaagaac
     3661 caaaagctac aggagacaag gttaaaataa aagtagaata tagtggtata tgtggttcag
     3721 atatacattc atttaaaggt gaatatgcaa atataaaagc cccagtaaca ttaggtcatg
     3781 aattttctgg aattgttgta gaagtaggag aagatgttaa agatataaaa gtaggagata
     3841 gagttacatc agaaactact tttgcaactt gtgagaaatg tatatattgt gaaactaaag
     3901 attataactt atgcccaact agaaaaggta ttggtactca agtaaatgga agttttgctg
     3961 agtatgtact atcaagagaa gaaagcatac atgtacttcc tgaaaatgta tctttattat
     4021 cagcagccct tacagagcca ctagcttgtt gtgttcatgc agctcttgaa aagactacta
     4081 tagaaagttc tgatacagtt ttaataatag gaccagggcc aattggttta ttattagctc
     4141 aagttgttaa atctcaaggt gctacagtta taatgtctgg tgttacaaaa gataaggaaa
     4201 gattagaaat agctaaaaat cttggagtag atagaactgt aaatgtaatg gaagaaagtt
     4261 tggcagatgt agtgaatgaa atgactgatg gaaatggagt aaacaaagga tttgattgtt
     4321 ctgggtttat gccagcagtt aacgaagcct taagactgct tagaaaaaaa ggtactttcg
     4381 tacaagttgg aatctttgct aaaaaattaa atgaaatgga ccaagaagca ataatccaaa
     4441 gagagttaca atatatagga agtagatctc aaaagccaag ttcttggatt acagcattag
     4501 atttaatggc aagtgggaaa gtaaatacag aagctttagt tactagaact gttggtttag
     4561 acgaatttaa agaaggtatt acagctttaa tgaacgggga agaaataaaa gtagcagtaa
     4621 aatcttaaaa aaacatttgt attattttaa aaaaaattgt aaaatctact gtaggataaa
     4681 aaaatgtaag gtcttaaaaa ataacaggag gaagaaagta tgagcactct tagagatcat
     4741 tctaataata ttagaaaaaa cataataaaa atggttgcag aagctaaaag cggacatcca
     4801 ggtggttcat tatcaatagc tgatatgttg acagtattat attttgataa gatgaacgta
     4861 tgtgcagaaa atccaaaaat ggctgataga gatagatttg ttctatcaaa aggtcatgca
     4921 gcaccagctt tatatgctac attagcagaa aaaggatttt tcccagaaga agaattaata
     4981 acactaagaa aatttggttc taagttacaa ggtcacccag acatgaaaaa agtagcagga
     5041 atcgatatgt ctactggttc tcttggtcaa gggctatctg cagcaaatgg tatggcactt
     5101 gcaggaaaat tagataacaa agactataca gtatatacta tattaggtga tggagaaatc
     5161 caagaaggtc aaatatggga agctgcaatg acagcatcac attacaaatt agataattta
     5221 attgcatttg ttgacttaaa tggattacaa atagatggtt ctaatgaaga agtaatgaat
     5281 gtaaatccag tagatgacaa attcaaatct tttggatgga atgtaatagt aataaatgga
     5341 cattcttttg aagaaatagg aaatgcagtt gatgaagcta aaaaagttac aggaaaacca
     5401 actgtaataa tagctaaaac agtaaaaggt aaaggtgttt catttatgga aaataatgct
     5461 gcatggcatg gaacagctcc aaatgcagaa caagcagagc aagcattgaa agatttagaa
     5521 ggaggtctat aggaatggga atagcaacta gagaagctta cggacaagtg ttaaaagaac
     5581 ttgcagaaaa taaagatata gtagtattag atgcagattt aggaaaagct acaaaaagta
     5641 tatcatttaa agaagttgcc ccagatagat tttttgacat ggggatagca gaaggtgaca
     5701 tgattggtac agcagcaggt cttgctactt gtggaaaaat accttttgcg agtacttttg
     5761 caatatttgc agcaggaaga ggctatgagc aaataagaaa ctctgtagca tatccaaatt
     5821 taaatgtaaa aatagcagct actcatgcag gtgtaacagt aggtgaagat ggtggtagcc
     5881 atcaagctat agaggatata tctctaatga gaggtatacc aaatatggtt gtattaaatc
     5941 cagcagatgc tttagaagca agacaagcta ttcttgcatc tattgattat aatggacctg
     6001 tttatataag attaggtaga gctgcaactc ctgatgtaaa cagtgaaaat tataaatttg
     6061 aaataggaaa aggaactgtt ttaagagaag gttctgacat aacagtaata gctacaggaa
     6121 taatggtagc aaaagcctta gaagcagcag aagaacttgc taaagaaggt ataaatgtag
     6181 aagttgttaa tatatctaca ataaaaccat tagatgaagc attaataaaa gaaactgcta
     6241 agaaaactgg aaaagtagtt acagcagagg agcacagtat aataggtggt ttaggttcag
     6301 ctgtatgtga agctttagca gaaactaaag atgtagtagt tagaagaatt ggtgttaaag
     6361 atgtatttgg tcaatcaggt acaccagctg atttattaaa acactatggt ttaactacag
     6421 aagatatagt aaaaaatata aaagagttgt tatagatata aatatatcta aataacaaat
     6481 tgatttgaca taagtcaatt aaagctgtct taaaaataga aagaaatttc tacaatggga
     6541 cagctttatt tttacaatac tttattttta tgataattat aaaatagtct gaaagtaatc
     6601 aaaaaaatta taaaaagagg tgctatttaa tgaaaatatc tattggatgt gaccatggtg
     6661 gttatgaatt aaaggaatat ataaaggaaa atttaataaa taaaggtata gaagttgaag
     6721 atgtgggtac aaattcaaaa gaaagtgtag attttccaat atatgcaaaa aaggtaagtc
     6781 aaaatgttca aagtaaaaaa tctgatttag gcatactttg ttgtggaaca ggaataggca
     6841 tgtctattgc agcaaataag tttaaaggaa tcagagcagc tgttgtttct gactgttttt
     6901 cagctaaggc aactagagaa cacaataata caaatgtact ttgtttagga gaaagagtta
     6961 ttgggaaagg attagcaatg agaattgttg aagaatggtt aaattcaagt tataatggag
     7021 aaagacatgc aagaagatta caaatgattg atgatataga aaaagaaaat ttataaatat
     7081 atataatata taatttataa caagggggag cgttatgtct attatttgtg cttcaataat
     7141 gtgtgcagac caaatgaagt taaaagagga attagaagct cttgaagaag caggtgtaaa
     7201 attacttcat tgtgatgtta tggatggaat atttgttaaa aacttggcta tgggaccaga
     7261 gttattaaaa tcaataagtg aaaatacaac aataccttta gacatacact tagccacaga
     7321 aactccagat aaatatattg atatgatgtc ttatataaag ccaaaatata tttcttttca
     7381 tgtagaatca agtacaaatg taaaagctga tatacaaaag ttaagaaatt atggtatagg
     7441 gccagtgtta gcaataagcc ctcaaacaag tgtggataaa atagaagaat atataagttt
     7501 agttgagggt atcttagtaa tgacagtaaa tccagggttt gcaggtcaaa agtttaatct
     7561 ttctgtattg gataagttgg ataaacttac agagatacta aaagattatg ataatcctcc
     7621 atttatagag gttgatggaa atataaataa ggatactatt aaacttatga atggtaaaaa
     7681 agtagatata tatgttgttg gtacttcagc tttatttaat gataagcctc caatttcata
     7741 taaagataaa atagaagaac taaaagaaag tataaagtaa ccatgtaata aaattataat
     7801 atgtttctgt ttaatagtaa aaatttaggt ggaaacaaga aattttataa tatttaataa
     7861 aaattttggt tgagtaagga gaatttttta tgggagacct atataaacta caaaatggta
     7921 ctgatattag aggaatagcg tatgaaaatg attctaaaga agttaaccta actgtagaag
     7981 aagtaaaaaa aatagctaaa gcgtttcata tttggctaaa agaaaagact aagaaagata
     8041 aagtaactgt tgccatagga actgattcta ggataacagg aagccaattt agaagtactg
     8101 ttattgaaac tttaactaat gatgattgta atgtaattga ttgtgagatt gctacaactc
     8161 cagctatgtt tatgacaact attatggatg gatacaattc tgatggggcg attatgatta
     8221 cagcaagtca cttaccttat tactataatg gtttaaagtt tttcacagaa agtggaggtt
     8281 tagaaaaaac taatataaaa gagatgttgg atatagctgt taaaaatgat agtgtagact
     8341 atgaggaagt aaataaaaaa ggtgaagtta tagtaaaaaa tttaatagaa gattattcta
     8401 atttattgat agataaaatt agaaaaggtg taaattcaag taaaaattat gaaaagcctt
     8461 tttctgggct taagatattg gttgatgcag gaaatggagc tggtggattc tttgctgaaa
     8521 aagttttaca catactggga gcagatacga caggcagtca gtttttgaac ccagatggta
     8581 tgttcccaaa tcatatacct aatcccgaaa acaaagaagc aatggaaagc atatgtaaag
     8641 ctgtgttgga taataatagc aacctaggaa taatctttga tacagatgta gacagagctg
     8701 ctattgtagg taaaaatggc aaaccaataa ataaaaatgc tcttatagct gttatatcat
     8761 caatagtatt agaagaacat ccaaaaacag ctatagtaac agattcgatt acttctgaag
     8821 gacttgctaa atttataaat gaattacaag gaagacatca tagatttaaa agaggatata
     8881 aaaatgtaat aaatgaagct ataagattga atagtgaagg tgaagagtgc catttagcta
     8941 tagaaacgtc tggacatgct gctttaaaag aaaattattt cttagatgat ggggcatatt
     9001 taatagctaa aattttaata aaagtagcaa agctatcctt ggaaggaaag actatagaag
     9061 aattgataga aaaccttgaa gaagctcaag aagagaaaga agttagaata ggcattaaca
     9121 aagaagattt tagaccatat gcagaggata tattaaaaga tttagattct catgttaaga
     9181 gtattgatgg atggtctgta gcaccaaata attttgaagg aataagagtt aattgtgatg
     9241 aatcaaatgg agatggatgg tttttactta gaatatcact acatgaacca ttactagctt
     9301 taaatattga atcaaataaa attggtggag cagaacaaat ttattctaag ttaaaacttt
     9361 tcttagaaaa atatgattta aaaggtcttt agggtaaata gaaagaatat atttatatta
     9421 aagaacaaat gtaaatttgt acctaagatt acaaatatta caaaattgta atcttaggta
     9481 acagagatgt aactgattat ggaaacacta tgtgatatta tagatacaga gagatgaaat
     9541 atgtgatgaa ttatagagaa atataattta aaatgtatta atatcacacc aaaaaggtat
     9601 aatcaaaaaa tatgtaaata taaaccccaa catttagaaa aagacctcta aggaggtttt
     9661 tttcttgttt aaataactta aaatatattt ttatattaat atatcaatta actcttctaa
     9721 aagtaagcat taccttaaat aaatcagcat ctataattat ttccatgtgt gcattttcta
     9781 attcaattat attttgagca attgataaac caagaccaga gccatctgtt tttctagata
     9841 tatcacccct tctaaatctt tctattaact cttctggctt tagatttagt ttatcatttg
     9901 aaatattttt aaacgttaat attatatttt cttcctttat aaacatatct atatagaccc
     9961 tagtgttagg catagagtat tttagtatat tgcaaattag attctcaaaa actctccaag
    10021 tttttttacc atctatcatg gtaaaaacag catattcagg tgtttctatt ataaaatcta
    10081 aactgctatt ttgaattttg tcttcaaact ctacaatact ttgaattaaa agttctctta
    10141 tatcggtttt tgttatatta aattctacat ctccactact tagcttagat gcttcaaata
    10201 tgtcatctat taataatttt aatctttgtg attttttatc taaaatattt atatagtcat
    10261 ttatagtctc tctgtcaaag tcattatttt ttaacatatc aacatagctt aatatagatg
    10321 taagtggagt ttttaagtca tgagatacgt ttgtgataag ctctgccttc atcctttcat
    10381 tttttatatt agcatctaca gcagtttcaa gaccacctgt tatattattt atacttttgg
    10441 ctaaagtaga tatttcatca tctcctataa cttctaattt atactttaaa ttgccatttt
    10501 ctatattttt tatatttttt gatatgttat ttatgtaatt aaacttaaca gataaaaagt
    10561 tgtatatata aaaatgaaaa actgtgttta caagtgtaaa taaaa
//
LOCUS       DAMJWP010000119        10245 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.124, whole genome shotgun sequence.
ACCESSION   DAMJWP010000119 DAMJWP010000000
VERSION     DAMJWP010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10245
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.124"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            331..1761
                     /locus_tag="PC700_002402"
     CDS             331..1761
                     /locus_tag="PC700_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2581704.1"
                     /translation="MSSQESKPKGNLISNFRDDMRDPKKRAIYTYSSSINLAYNFIGY
                     FVSTVTNIFLTDVAKLGVIMAGGMQIIQSFIKIAVAPITGTVFDKQPFKKGKYYPWLR
                     YAPAALALTYGIFFIVPLMKISPALLLPLMTALLLIASCAQQVVFTIIYAIYPVVAKT
                     PRDRVIGSTTTNIFKEVGKFLVGFSYPLLLVLFADVLGGEGMGYLGTYLFFAAGCLLV
                     FWFSSTEIIKSGAEEEVLQKEEKPKIKASEMAKALFTNVPLMMAFCLEFLICIRSIGL
                     GPLAPYYFKYVIEDERGLAVFLSIMPLVSVAFMFFAPVFIKICREQKLASIISFSICA
                     LCHLLVAWTPWGKTTLGVTMLLAIGGGFSNVVSIINLNFFAGSCDYGHWKSGKDLPGL
                     SMSLYPVAIQVGVLLATTIRTVLMNSMGYQADMVVTEAVKSGFINMISYSMAIPLIIA
                     VVIAILYPVSDKKLNQIREELNQRNA"
     gene            1864..2967
                     /locus_tag="PC700_002403"
     CDS             1864..2967
                     /locus_tag="PC700_002403"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDF2581705.1"
                     /translation="MIEKLLRNTVKELHQYVPGEPIEKVKEKYGVKEIIKLASNENPL
                     GPSPKAIEAMIEMLKQGQLYPEPEANELRRKLAEKLDLKPENFIVANGADNVITLIGE
                     AFINRGDEVIYCNPTFPSYRTATIKNEGIPVEVPLTEDYKYDLQGILDKITDKTKLIC
                     VCNPNNPTGTIVDDKELEEFLKKVPENIITILDEAYIEFLTVPNYVDGLKYVRENYNV
                     IVTRTFSKIYGLAGLRVGYGIAKDEIIRTLFTVKEPFSANRVAISGATAALDDEEFIK
                     ETYELNRVGMTYFKEEFTKMGFDVVDSQSNFLYVDMKTDIPKLFEDLKKRGFVIRPSA
                     THARVSIGTMEENKKFVAVLKEILNIKGEPSIG"
     gene            2982..3218
                     /locus_tag="PC700_002404"
     CDS             2982..3218
                     /locus_tag="PC700_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2581706.1"
                     /translation="MKRGVDMNLKINAERCKSCEYCVISCKKGALKISSKINKEGYAH
                     VEVDESKCILCGICYQVCPDNVFEIIKEKACQEA"
     gene            3235..4284
                     /gene="vorB"
                     /locus_tag="PC700_002405"
     CDS             3235..4284
                     /gene="vorB"
                     /locus_tag="PC700_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HDF2581707.1"
                     /translation="MKKMWKGNHAIAEAALRGGCEFYAGYPITPQTEVMEFLSHRMSE
                     LGRTFIQSENEMAAIYMVYGAYASGMRSMTSSSGPGISLKQEGISYLCANHYPCVIVN
                     VQRWGPGLGSLDSAQTDYLRDTRGGGNGDYHLIVYAPNSIQETVDLMYNSFDVAEKYR
                     VPVEILTEAALGQMMEPVEFPEFKKREGDLGWTYDGSNRDHAKVADNQKPTFCMEKRM
                     RITENEQQWEDYQVEDAEYVFVAFGIPSRTTMNAVRRLREQGEKVGIIRPITVWPFPY
                     KAFEKVSKDVKGFISVESTDTGQLVEDVALASKKVCKENVPVYGLFSGNHIPKTLQVM
                     DTYSKIKSGEIKEVF"
     gene            4286..5026
                     /locus_tag="PC700_002406"
     CDS             4286..5026
                     /locus_tag="PC700_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HDF2581708.1"
                     /translation="MATEKLTPSVVSIPNKFCPGCGHGIVNRIIAEVIEEHGYEKNHV
                     LTLGVGCVCNMNFSWNGDKMQTAHGRASSTAIGVKVALPDTLVMTYQGDGDAYVIGLS
                     ETLNTAYRNHNVTVFVINNNNFAMTGGQMSWTTMPGQVTTTSVNGRDIKTTGSPLKVP
                     EMVANFFDVAYVARGSVHSPKEIINLKKYIKNAIEAQLNGEGYSLVEILAPCPTNWGV
                     SLEKSIKWMEEEIVPYYALGEFKQRDGE"
     gene            5029..5568
                     /locus_tag="PC700_002407"
     CDS             5029..5568
                     /locus_tag="PC700_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HDF2581709.1"
                     /translation="MEKELVFAGFGGQGVLTLGLIVAESALELGKQVTWMPAYGPTMR
                     GGKAYSVVKFSDESIGGPDMEEIDVLVAMNKPSLDYINLVKEGGTVVINTSAIDENVT
                     LREDISVVKINCQELAQKVNNPKAANIVVLGALIAKTGLLEKELALKTMCDFFEEKGK
                     GKFNVQNEAAFMEGYNNAI"
     gene            5692..7791
                     /locus_tag="PC700_002408"
     CDS             5692..7791
                     /locus_tag="PC700_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HDF2581710.1"
                     /translation="MSLEKLIRPKSIAIVGVTDKLGFGRSAALSIVKSKETDRVYYVN
                     PKREELFGRKCYKMIQEVPEVVDCVVVCTPRNVVPSVLKDSGELGTKAAVVYASGFAE
                     EGTEEGTDLENQLIEISNTYDMKILGPNCMGLLNCIDKVNLWAGGSKWALDTKKPGIG
                     IVGQSGFITAEIVSSDYFNISYGFSTGNGNIVTLEDAVDFLVDDDYASVIAIYLEGLK
                     NPQKFIDALKRAAQKRKPVIILKSGRSEKGAISAASHTGNLAGSSKAFESIFEKYGVI
                     SVENLEQFMCLAQAFSVLDGNLPANSNFAAINFSGGENTICADLAEENGVELAEISSE
                     TKEEMKKYLPGFATPKNPLDATTALFHEKDMIVGLLHTFEKDDSVGFTMVGANIRDEE
                     NEMHETLCKAVSEAREQGLKKPVFAVPTLEGNRYLDYRPRLEDNQVPIMSSVGTTFTC
                     FNKMAKFIDYDYSKRTLEFKAVKKRESNNVVALSELDSKIEMKKYGVPVPGQGNTKSI
                     DELDEILKYIKYPVVLKINSSEILHKSDVGGVKIGIKNRDEAVDAYNEILTNVKKAKP
                     DANIDGILVQEMVESGIEIIIGITNDDQFGPMLLVGLGGVFVEVFKDTTLYPLPINHD
                     EAIMMLKKLKSFKLLNGYRGSEPCDIDALADMMVKLGKYAYENKDEVKEIDLNPVFVY
                     PKGKGVCAVDALIVKYK"
     gene            complement(8058..8573)
                     /locus_tag="PC700_002409"
     CDS             complement(8058..8573)
                     /locus_tag="PC700_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HDF2581711.1"
                     /translation="MISAKMEKLLNEQINHELYSAYLYLSMSSYLEAEGLKGFSNWFY
                     VQYKEETDHAMFFYKYLHNVGGKVQLDAIPMPDSDFTSAMDILERTLAHEKKVTALIN
                     NLAAVANSESDFRTSQFLLWFISEQAEEETNCEDNIKRVKLAGEGGLFFVDQEFANRT
                     YAAPTNPPVTI"
     gene            complement(8665..9876)
                     /locus_tag="PC700_002410"
     CDS             complement(8665..9876)
                     /locus_tag="PC700_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HDF2581712.1"
                     /translation="MSNKQGSNIKDVKIKNKLIILKLLSTNTPMSRVDLAKSTGLTKM
                     TLSNLVTELINENMIREIESSTLNAHSSGRKPILLDISNISPCICGILIRRNICKVII
                     SDLKGNIVKQLSETYPKCLSNDVLKDIIKRMFTKIMKINKRDIIGIGISSIGPVDDIN
                     GTILNPPDFGNVSNLNIVDFIKEMSDFPIFLINDANSGALAEKMYGLGKNISNYIYLH
                     IMNGIGAGLVLENKLHTGNLGQSGEIGHTSINFSGPLCSCGNNGCLDYYTSVSNLIKR
                     IESLSHIYPNSPLINCKDFSLVKLIDEANNKDSLAMFLLDEFCTYVSYALVNTLTLID
                     CSSIIIGYDFNIPGTFIENTLLKKLTASASFSKYKKISVRHSNFGANAPLIGSIAIVS
                     YNFFNGSINFY"
BASE COUNT         3883 a         1254 c         1864 g         3244 t
ORIGIN      
        1 attattcttg aataaaatta taaaaattta aaatcactag aaatatgata aaataattta
       61 aaatacgcat ttcaatgaca tttcaagaca agaaaataat aaaaaacaaa tctaaattaa
      121 gcctaaatag ctttttagtt aattttagta gtaaaaaata aaacttaaaa agtgtttgac
      181 aattatgaat tttaagaata taattaaatt aaaattatac acatataaaa atatactcct
      241 gaatcaagaa tgagatattt aagattcaaa atatatatta ttatacgtga ataatgaaaa
      301 gtaagaaaat tttataaggg aggggtagat gtgagtagtc aagaaagcaa accaaaagga
      361 aatcttatat caaattttag agatgatatg agagacccta agaaaagagc aatatacaca
      421 tattcaagtt ctataaactt agcgtacaat tttataggtt actttgtatc aactgttaca
      481 aatatttttt taactgatgt ggcaaaactt ggtgtaatta tggcaggtgg tatgcaaatt
      541 atacaatctt tcataaaaat tgctgtagct cctataacag gtacagtatt tgacaaacaa
      601 ccattcaaaa aaggaaagta ttatccatgg cttagatatg caccagcagc gttagcatta
      661 acatatggaa tatttttcat agttccatta atgaaaataa gtccagcatt acttttacct
      721 ttgatgacag ctttattatt aatagcatct tgtgctcagc aagtagtttt cactataatt
      781 tatgctattt accctgtagt tgctaagaca cctagagacc gagttatagg ctcaactact
      841 actaatatat ttaaagaagt tggtaaattc ttagtaggtt tttcatatcc gttattatta
      901 gttttatttg cagatgtatt aggtggagaa ggaatgggtt atttaggtac ctacttattc
      961 tttgcagcag gatgcctttt agtattttgg ttttcttcaa cagagataat aaaatctgga
     1021 gcagaagaag aagttcttca aaaagaagag aagccaaaga ttaaagcaag tgaaatggca
     1081 aaagctctat tcactaatgt accacttatg atggcatttt gtttagaatt tcttatatgt
     1141 ataagatcta taggattagg acctttggct ccatattatt ttaaatatgt aatagaagat
     1201 gaacgtggat tagcagtatt tttatcaatc atgcctttgg taagtgtagc attcatgttt
     1261 ttcgcaccag tgtttataaa aatctgtaga gagcaaaaac tagcttcaat aatttctttt
     1321 tctatatgtg cactatgcca cttattggtt gcatggactc cttgggggaa aactacttta
     1381 ggagtaacta tgttgctggc aattggtgga ggtttttcaa atgtagtatc aataataaat
     1441 cttaatttct ttgccggttc ttgtgattat gggcattgga aatcaggaaa agacttacct
     1501 ggcttaagta tgtcactata tccagttgca atacaagtag gagtattact tgcaactact
     1561 ataagaacag ttttaatgaa ctcaatggga tatcaagctg atatggtagt cacagaagca
     1621 gttaaatcag gttttataaa tatgatttct tattctatgg caataccttt aataatagct
     1681 gttgttatag caatactata tccagtaagt gacaagaaat taaaccaaat aagagaagag
     1741 ttaaatcaaa gaaatgctta gttaaaagaa agttaagtag atggttaatt taagctatta
     1801 aagtaagtag acgcaatagc gtttatataa atcaaaatac atatacagag ggggctttta
     1861 aaaatgatag aaaaattatt gagaaatacc gtaaaagaat tacatcaata tgttccaggt
     1921 gaaccaatag aaaaagttaa ggaaaaatat ggtgtaaaag aaataataaa attagcttca
     1981 aatgaaaatc ctctaggacc atcaccaaaa gcaatagaag ctatgattga aatgctaaaa
     2041 caaggtcaac tttatccaga gccagaagct aacgaactta gaagaaaatt agctgaaaag
     2101 ctagatttaa aaccagaaaa ttttattgtt gctaatggtg cggataacgt tatcactcta
     2161 ataggtgagg cttttataaa tagaggagat gaagttatat attgtaaccc aacatttcca
     2221 tcatatagaa ctgcaactat aaaaaatgaa gggataccag tagaagtgcc tttaacagaa
     2281 gattataagt atgatttaca aggtatatta gataagatta cagataaaac aaaattaata
     2341 tgtgtatgta accctaataa tccaacagga acaattgtgg atgataaaga gttagaagaa
     2401 tttttaaaga aagtccctga aaatattata actatattgg atgaagcata tatagaattt
     2461 ttaacagttc ctaattatgt ggatggactt aagtatgtaa gggaaaatta taacgttata
     2521 gtaacaagaa ctttctctaa aatatatggt cttgcagggc ttagagtagg atatggtatt
     2581 gctaaagatg agataataag aactttattt acagtaaaag agcctttctc tgcaaatcgt
     2641 gttgctataa gtggagcaac agcagcactt gatgatgaag agtttataaa agaaacatat
     2701 gaattaaata gagttggaat gacttacttt aaagaagagt ttacaaaaat ggggtttgat
     2761 gttgtagatt cacaatcaaa tttcctttat gtagatatga aaacagatat acctaagtta
     2821 tttgaagact tgaaaaaaag agggtttgta ataagaccaa gtgctacaca tgcacgtgta
     2881 agtattggaa ctatggaaga aaataagaaa tttgtagcag ttttaaagga aatattaaat
     2941 attaaaggag agccaagtat tggataatag atagaaatat tatgaaacgg ggtgtagata
     3001 tgaacttaaa aataaacgca gagagatgta aaagttgtga atactgcgtt atatcttgta
     3061 aaaaaggagc attaaaaata tcaagtaaga taaataaaga aggttatgct catgtagaag
     3121 ttgatgagag taaatgcata ctttgtggaa tttgctatca ggtgtgtcca gataatgttt
     3181 ttgaaataat taaagaaaaa gcatgtcaag aggcttagaa ggagttggga taatatgaaa
     3241 aagatgtgga aggggaacca cgccattgca gaagcagccc taagaggagg ttgtgaattt
     3301 tatgcaggat atccaattac accacaaaca gaggtaatgg aatttttatc acatagaatg
     3361 tctgaacttg gaagaacttt tatacagtcc gaaaatgaga tggctgctat atatatggta
     3421 tatggagcat atgcaagtgg aatgaggtct atgacgagtt cttcaggacc aggaatatct
     3481 ttaaaacaag aagggatttc ttacttatgt gcaaatcatt acccttgtgt aatcgtaaat
     3541 gtacagagat gggggcctgg tcttggctca ttggactcag cacagacaga ctatctaaga
     3601 gatactagag ggggaggaaa tggagattat catttgatag tttatgcacc aaacagtatt
     3661 caagagactg tagatttgat gtataactca tttgatgtag cggaaaaata cagagtgcct
     3721 gtcgaaattt tgacagaagc agctttaggt caaatgatgg aaccagtaga atttccagaa
     3781 tttaagaaga gagaaggaga tttaggttgg acttatgatg gaagtaatag agaccatgca
     3841 aaggttgcag ataaccaaaa gccaactttt tgtatggaga aacgtatgag aataactgaa
     3901 aatgaacaac aatgggaaga ttatcaagta gaagatgcag aatatgtgtt tgtagcgttc
     3961 ggtataccat caagaacaac tatgaatgct gttagaagac ttagagaaca aggtgaaaaa
     4021 gttggaataa taagaccaat aacagtatgg cctttcccat ataaagcatt tgaaaaggta
     4081 agtaaagatg taaaaggatt tatctcagta gaatcaacag acacaggtca attagtagaa
     4141 gatgttgctt tagcctcaaa gaaagtatgt aaagaaaatg tacctgtgta tggtttattt
     4201 agtggaaatc atataccaaa aacattgcaa gtaatggaca cttatagcaa aataaaatct
     4261 ggcgaaatca aggaggtgtt ctaatatggc tacagaaaaa ttaacaccta gcgttgtttc
     4321 aataccaaac aagttttgcc caggttgtgg tcatggaata gtaaatcgta taatagcaga
     4381 ggtaatagaa gaacatggat atgaaaaaaa tcatgtatta acattaggag ttggttgtgt
     4441 ctgcaatatg aattttagct ggaatggtga taagatgcaa actgcacatg gtagagcttc
     4501 atctacagct ataggagtta aagtagcact tccagataca ttagtaatga cttaccaagg
     4561 tgatggagat gcttatgtta taggtctttc tgaaacttta aatacagcat atagaaatca
     4621 taatgtgact gtatttgtaa taaacaataa taattttgca atgactggtg gacaaatgtc
     4681 atggaccact atgccgggtc aagtcacaac aacaagtgta aatggaagag atataaagac
     4741 tactggtagt ccactgaaag ttcctgagat ggtggcaaac ttctttgatg tagcatatgt
     4801 agcaagaggt tcagttcata gcccaaagga aataattaat ctaaaaaaat atataaaaaa
     4861 tgcaatagaa gctcaattaa atggagaggg atattcctta gttgaaatat tagcaccttg
     4921 cccaactaac tggggtgtat cactagagaa atctatcaaa tggatggaag aagaaatagt
     4981 gccatattat gctcttggcg aatttaagca aagggatggt gaatagttat ggaaaaagag
     5041 ctagtatttg cagggtttgg tggtcaagga gttttaactc ttggtctgat tgtagctgag
     5101 tcagcacttg aattggggaa acaagtaact tggatgcctg catatggtcc aactatgaga
     5161 ggtggaaaag cctatagtgt agtaaagttt tctgatgaga gtataggtgg gcctgatatg
     5221 gaagaaatag atgttttagt agcaatgaat aaaccatctt tagattatat aaatttagtt
     5281 aaagaaggtg gaacagtagt tattaataca tctgcaatag atgagaatgt aacattaaga
     5341 gaagatataa gtgtagttaa aataaattgt caggaattag ctcaaaaagt aaacaatcca
     5401 aaagcagcaa atatagtagt acttggagca ttaattgcta aaactgggtt acttgaaaaa
     5461 gaattagctt taaaaactat gtgtgatttc tttgaggaaa aaggaaaagg aaagtttaat
     5521 gttcaaaatg aagcagcatt catggaaggc tataataacg ccatttgata aattaccctt
     5581 atcttaagtg atttatctca agatttaata atctttagtg ccaagagaaa cttcatcttg
     5641 agataaaatt cacttaaaat cacaactata aacatatagg agcgtgaatt tatgagtttg
     5701 gaaaaactta ttagaccaaa aagtattgct atagtaggag tgactgataa acttggtttt
     5761 ggaagaagtg cagcacttag tatagtaaaa agtaaggaaa cagatagagt atattatgtt
     5821 aatcccaaaa gagaagaatt gtttgggaga aaatgttata agatgataca agaagttcca
     5881 gaagtagtag actgtgtagt agtatgtacg cctagaaatg tagttccatc tgtattaaaa
     5941 gattctggag agttaggaac aaaggcagca gtggtatatg ctagtggatt tgcagaggaa
     6001 ggtacagagg aaggtacaga tttagaaaat caacttatag aaatatcaaa tacatacgat
     6061 atgaaaatat tgggtccaaa ctgcatgggg ttgctaaatt gtatagataa ggttaatcta
     6121 tgggcaggag gttctaaatg ggctttagat actaaaaaac ctggtatagg aattgtaggt
     6181 caaagtggat ttataactgc tgaaattgtt tcaagtgatt attttaatat atcttatggt
     6241 ttttcaacag gaaatggaaa tatagttaca ctagaagatg cagtagattt ccttgtagat
     6301 gatgattatg catcagtaat agcaatttac cttgaaggtt taaaaaatcc tcaaaaattt
     6361 atagatgctt taaaaagggc agcacaaaaa agaaagccag taataatatt gaaatcagga
     6421 aggtcagaaa aaggtgctat ttctgctgcc tctcatacag gaaatttagc tggttcgagt
     6481 aaagcttttg aaagtatttt tgaaaaatat ggagtaattt ctgtggaaaa tttagaacag
     6541 tttatgtgtt tagcacaagc ttttagtgtt ttagatggta atttacctgc aaattctaat
     6601 tttgcagcaa taaacttctc aggaggagaa aatactattt gtgcagattt agcagaagaa
     6661 aatggtgtag aacttgcaga aatatcttct gaaactaaag aagaaatgaa aaaatatctt
     6721 ccaggatttg caacacctaa aaatccacta gatgctacaa cagcattatt ccatgaaaag
     6781 gatatgatag taggtttatt acataccttt gaaaaggatg atagtgttgg atttactatg
     6841 gtaggtgcaa atataagaga tgaagaaaat gaaatgcatg aaaccctttg taaagctgta
     6901 agtgaagcta gagaacaagg attgaaaaaa cctgtgtttg cagttcctac acttgaagga
     6961 aatagatatt tagattatag gcctagatta gaagataatc aagtgcctat aatgtcatca
     7021 gttggaacta catttacttg ttttaataaa atggctaagt ttatagatta tgattattca
     7081 aaaagaactt tagaatttaa agctgttaag aaaagagagt caaataatgt agtagcatta
     7141 tcagaattag atagtaagat tgagatgaaa aaatatggag taccagttcc aggacaaggt
     7201 aatacaaaaa gtattgatga attagatgaa atcttaaaat atattaagta tcctgttgtt
     7261 ttaaaaataa attcttctga aatattgcac aaatctgatg ttggaggagt taaaatagga
     7321 attaagaata gagatgaggc agtagatgca tataatgaga ttttaactaa tgtaaaaaaa
     7381 gcaaaaccag atgctaatat tgatggtata ttagttcaag aaatggtaga aagtggtata
     7441 gaaattatta ttggtataac taatgatgac caatttggac caatgttatt agtcggttta
     7501 ggaggagtat ttgtagaggt atttaaagat actacattat atccattacc tataaatcat
     7561 gatgaagcta ttatgatgct taaaaaacta aaatcattta agcttttaaa tggctataga
     7621 ggaagtgagc cttgtgatat tgatgctctt gcagatatga tggtcaaatt aggaaaatat
     7681 gcttatgaaa ataaggatga agtcaaggaa atagacttaa atccagtgtt tgtatatcct
     7741 aaaggaaaag gcgtatgtgc agtagatgcc ttaatagtta aatataaata gtttataagt
     7801 tttggtattc aaccataatt aaagcaccga ctgaaaatca cattttgtaa gttagatagt
     7861 gtgattttca gtcaccccaa atttaattta aaaatatttt aaaatgaaag tatagttttg
     7921 agtttaattg tgaaatagca ctgtataaac aataaaatac tacgtataaa acgaataata
     7981 aaaggtgttt taatgataag aatcaatctt gattttcatt aaaacacctt ctttattata
     8041 ttaaaaattt ttaaacgtta tattgtcaca ggtgggtttg ttggagctgc atatgtacga
     8101 ttagcaaatt cttggtcaac aaagaataga ccaccctctc cagcaagttt aacacgtttt
     8161 atattgtctt cacaatttgt ttcttcttct gcttgttcag atataaacca aagtaagaat
     8221 tgagaagttc taaagtctga ttcagagttt gctacagctg ctaagttgtt tattaaagct
     8281 gtaacctttt tttcatgagc taacgtacgc tcaagaatat ccattgcaga agtaaagtct
     8341 gaatctggca ttgggatagc atcaagctga acctttcctc ctacattatg taagtatttg
     8401 taaaaaaaca tagcgtggtc tgtctcttct ttatattgaa cataaaacca attagaaaat
     8461 cctttaagtc cttctgcttc aaggtaagaa gacatagaaa gataaagata cgcagaatat
     8521 aattcatggt taatctgctc attaagtaat ttttccattt tagctgaaat catttttata
     8581 cctccaaatt ttaaatgctc ttataaaata taataatatt aaatggaaaa atattcaata
     8641 aaagtgttta aatattttac atatttaata aaaatttata ctaccattaa aaaaattata
     8701 acttacaatg gctatagagc caattaatgg tgcatttgct ccaaaatttg aatgccttac
     8761 acttattttt ttatatttag aaaaacttgc tgaagcagtc aattttttta ataatgtatt
     8821 ttctataaat gtaccaggta tattaaagtc atagcctatt attatagaac tacaatcaat
     8881 caatgtaagt gtattaacta atgcatatga tacgtatgta caaaattcat caagcaaaaa
     8941 catggcaaga ctatctttat tatttgcctc atctattaac ttgactaaag aaaaatcttt
     9001 acagttaatt aatggggagt taggataaat atggctcaat gattcaattc ttttaattag
     9061 attactgaca cttgtataat aatctaaaca tccattgtta ccacaactgc acaatggtcc
     9121 actaaaatta attgatgtat gtcctatttc tccactttgt cctaggttgc cagtatgaag
     9181 tttattttct agaactagac cagctccaat accattcatt atgtgtaggt atatataatt
     9241 tgatatattt ttacctaaac catacatttt ttctgctaat gcacccgaat tagcatcatt
     9301 tatcaaaaat attggaaaat cagacatttc ttttataaaa tctactatat ttaggtttga
     9361 tacatttcca aagtctgggg gatttagtat ggtaccattt atatcatcta ctggacctat
     9421 tgaagaaata ccaattccaa ttatatctct cttgttgatt ttcattattt tagtaaacat
     9481 tctttttatt atatctttta aaacatcatt tgatagacat ttagggtaag tttcagaaag
     9541 ttgttttaca atattacctt ttaaatctga aataattacc ttacatatat ttcttcttat
     9601 taggattcca caaatacaag gcgatatatt agaaatatct aataaaatag gtttacgacc
     9661 tgatgaatgt gcatttaaag ttgatgactc tatttcccta atcatatttt cattaataag
     9721 ttcagtaact agatttgata aagtcatttt agtaagtcct gtagacttag ctaaatcaac
     9781 tctggacata ggtgtatttg tagaaagtaa tttcaatatt attaacttat ttttaatttt
     9841 tacatctttg atgttagaac cttgtttgtt actcatcata aatctcctta aataaagtta
     9901 agtgttttat ttattttcaa gtatactatc accttataat agagtaacat ttttttactt
     9961 gaaaatatca ttaattagaa tatatcacat tatatatcat aaaacatata aaaaaatgtt
    10021 agaattaaat taaaagtgaa aagtagattg atatataaat agtaaatact taattaaaaa
    10081 aagataatga tagcaataat tattgttacc aagaatctac tacttcaagt gttaccttag
    10141 gtatttaagt tgatagtggt ttgcacgtaa taaacttgat gtaatatata ttcttaataa
    10201 tatcgataaa agatttactt aaaaataata tagattatgt aaaca
//
LOCUS       DAMJWP010000120        10225 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.125, whole genome shotgun sequence.
ACCESSION   DAMJWP010000120 DAMJWP010000000
VERSION     DAMJWP010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10225
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.125"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            180..1604
                     /locus_tag="PC700_002411"
     CDS             180..1604
                     /locus_tag="PC700_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HDF2581713.1"
                     /translation="MSSSNQSTIKTSIQRVGKFLSGMVMPNIGAFIAWGLITALFIPT
                     GWMPNEKLSTIGDPMIKYLLPLLIAYTGGKAIAGQRGGVIGAAAAMGVIVGADIPMFI
                     GAMIMGPFAGWVIKKFDKFVDGKIPTGFEMLVNNFSIGIIGMLLAILGFYAIGPAIVA
                     GTALIESGVQFIVSKSLLPLVSVFIEPGKVLFLNNAINHGILGPIGIAEAKEAGKSIM
                     FLLESNPGPGLGVLLAYWMFSRGSVKQSAPGAVIIHFFGGIHEIYFPYILMNPVLILA
                     TMAGGAAGILTFSILGAGLVAAPSPGSIFALMALAPKGGLLPVLAGVAVATVVSFLVA
                     APFVKRASANQSEEDSTSLEEAKAKMSDMKSASKNSEKNIEEKQLEVNEIKKIVFACD
                     AGMGSSAMGASRFKNRIKNLDLNIEITNSSVDNLPDDTQIVVTHNTLVERVAKNNSSV
                     EIVSINNFLNDPNLDALFKRLESK"
     gene            1639..3768
                     /locus_tag="PC700_002412"
     CDS             1639..3768
                     /locus_tag="PC700_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDF2581714.1"
                     /translation="MKKKKITSRQKKIILMIVENSKKNIPITISEIAGTLELSSRTVL
                     RDMSGIEKWFDENDFNFVKKPGVGLILEENIENQNFIIELLEEEKIEKEYSKEERNLI
                     ILSKLLVSNEPVKSYYFAKILKVSEGVLNNDFALASKWLERFDIELVRKPGLGVYLKG
                     QEKNFREAYVNLIYDSFNEKEILDMVRNISENIQTDKAIEILSENRLLNLMDRCIIRK
                     VEATLTKKLSDLDVNLADSAYIGLVVHISLALQRIKNGENITMDKEFLKELSITEEFK
                     LAKEIVKGMETDFNMDIPVDEVGYITMHIRGAKQRSSSNHKALNLDDIEIMEITNKMI
                     DLAEDEFKISLKNDERLFKDLANHLGPSINRLNMGLEIRNPLLDEIKSKYSYAYDGVE
                     KISRIIKDKLNINSIPESEIGYIAMHFASAIEKNLMMNTNINIVVACPTGIGTSRFLS
                     TKIENKFPNLNILETISAINIDEEYLKEKDVDLIVSTVELNTSLNYICVGPFMSLDDE
                     QIIKEKIKSIAQNKLINLNAKNDTKSKNKVYEQITESMNIGKDILQFLEEIRFEKFES
                     KDLSELIEDSSKIFAKSSEDIISIKESLKERLKISIPYIEESKILLLHCMSERIDIMK
                     LSIIKLENRIVLDSNEEIDNVVFMLLPKNSPSYQRQIMSEISGSLIDNIIFTNKINKF
                     SIEEMTLEVKDIVFNFYTNRLKAFIDK"
     gene            3798..4220
                     /locus_tag="PC700_002413"
     CDS             3798..4220
                     /locus_tag="PC700_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2581715.1"
                     /translation="MSKVLNENNIFLGLDSVSKEEAITLAGRKLVENGYVKEEYIPAM
                     LEREKVMTTYMGMGVAIPHGVNEAKKEILSSGIVILQFPNGIDFDGEKAYLLIGIAGV
                     GDEHLEILSNIAVVLDDDLTERLKNSNDKQAFMEAFAN"
     gene            4239..5390
                     /locus_tag="PC700_002414"
     CDS             4239..5390
                     /locus_tag="PC700_002414"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF2581716.1"
                     /translation="MKKAIQFGAGNIGRGFIGSLLVKSGYHVVFADVNEEILNSINKD
                     KKYTIHIRDVECIDEVIDNISAVSSIKEEIIDEIVQAEIITTAVGPLVLTKIASTIAK
                     GIKARKEKGLTSNLNIIACENAIYASSSLKEEVLKYLNKEEVEYLEMYVGFPNCSVDR
                     IVPPGKNENPLDVTVENFYEWNVEKQGFKGEIPTIVGMNLADNLMAYIERKLFTLNTG
                     HAITAYIGYLKGYKTIEESIKDKFICDIVKSAMVESGEGLIKKYNFDSEVHYKYIDKI
                     LNRFKNPYLNDDVLRVGREPLRKLSDKDRLIKPLMTAKSYGLSVDNLILGIGAALHYN
                     NSEDTQSVELQELIKSIGVKKAVAKIANISNDEEILNNIEKSYIFMKNL"
     regulatory      5496..5597
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            5780..6352
                     /locus_tag="PC700_002415"
     CDS             5780..6352
                     /locus_tag="PC700_002415"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDF2581717.1"
                     /translation="MKALQEKILREGSVSGNDILKVDSFLNHQIDVAFLNEIGREFKE
                     RFKGEKVDKIFTIEASGIAIASIVSQYFDNAPVVFAKKSESKNLDKDVYETNVYSFTK
                     AREYSVKVSKKYINKGENILIVDDFLANGRAALGLKDLIEQAEANLVGVGIVIEKGFQ
                     AGGALLKANDVRLESLAVVESIDNGTVKFR"
     gene            6416..7066
                     /gene="fsa"
                     /locus_tag="PC700_002416"
     CDS             6416..7066
                     /gene="fsa"
                     /locus_tag="PC700_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDF2581718.1"
                     /translation="MRFFIDTANIEEIKEANDLGVICGVTTNPSLIAKEGRDFIEVVK
                     EISEIVDGPISAEVISLEHKGMIEEAEKLSKIHKNIVIKIPMTAEGLKAVKVLSSKGI
                     KTNVTLIFSAGQALLAARAGATYVSPFVGRLDDISQNGLDLIEEIVDIFSVNSIEAQI
                     IVASVRNPIHVLQAARMGADIATVPLKVINQMIKHPLTDKGIDSFMKDWEGAFLKL"
     gene            7218..10142
                     /locus_tag="PC700_002417"
     CDS             7218..10142
                     /locus_tag="PC700_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2581719.1"
                     /translation="MDKILREIQREDKKNPYTDQELAEMLNVARSEVISVRKKNNILD
                     SRERRKKILIKDISKIVSENPQMSERKITEILIENGYNISRSAVSKLLKEENLNQSKI
                     GIKDKDPASSGKYVKVSKVVEEDGFEELIGIKGSLKEKVNLAKSAIMYPPNGLHTIIY
                     GETGVGKSELATCMYKYAIKNNIKEENSPFIVFNCADYAENPNLLIAQLFGVVKGAYT
                     GADANREGLVEQANNGILFLDEIHRLPAAGQEILFSLIDRGEFRRLGESSSNRKANVL
                     IISATTENPDSNLLQTFRRRIPMVINLPNMSERPKSERYEIIIKFFEREAKRVNKNFI
                     ITKEVMCALMNYKCTGNIGQLKSDIQVTCARAFSKSIFSSDKVIIDLDSLRDYIKSGY
                     YDCNCKNEKYSEFSVEDIYIDISELVSGKNRILSDNEISEIYNYAEKELKVLESKCFS
                     EEELKNAFIEKLDKKFNAIKNNKNLSDRRSRIDWGGQLKESTLEIMDKVIVFIKEQFK
                     HVNQGLYLALAIHIEHAIGRIKDGKTIINPSLDKIKASMPMEYELSRYIMGIVEDLTQ
                     VTFPEDELGYLAYYINKFCYNEESIKDKVKVVIVTHGKVGIEMSKVVNHILGIECTLG
                     IEIALTDSPSEGIEHVLEELQKIEARKGILVLIDMGSLVILGDEVEKRLGIRCKTVNR
                     VDTLLAMEAGKLATIEGKSLDGIIADLKKNKNYAMINTNKFSYRKNEYGKKNVIITLC
                     LSGVGTALNLKEHIEKQIKEYGTLIEVKPVAFLNNNLEEELNDIEAEYNIIAICGTID
                     IDYKNVPFISYNEVLGKNGINKVLEHLNSKQPALNIENQLNNLIHEDLILYNFEGISK
                     EYIIDTLVSKLEEGGYVDSKYILSVYKREAMGSVVMASKVAVPHGLPENVIKPAIAIA
                     RLNKPIVWDNKFMVDLVVLLALKENNKKEIRSLFSKINDQHTLEVLLNTEDKNEIKEL
                     LS"
BASE COUNT         4197 a         1000 c         1934 g         3094 t
ORIGIN      
        1 atactccaaa atactacata aataaaatta aacaaaaact aaaatgacaa atgtcattat
       61 ctaaagtgac atagaaacgg tttcttaaag ttgttactat aaaatataaa ctctataata
      121 aaagtataac aaaagggaat catatcgata tagaatataa ttcaagagga ggtaaaatta
      181 tgagtagttc taatcaatcg acaataaaga catctataca gcgagtaggg aaatttttaa
      241 gtggtatggt aatgccaaat ataggagctt ttatagcatg gggtcttata acagcattat
      301 ttataccaac aggatggatg cctaatgaaa aattgagtac tataggcgac ccaatgataa
      361 aatacctttt accactattg atagcttata ctggtggtaa agcaatagca ggacaaagag
      421 gtggcgtaat aggtgcagca gcagctatgg gagtaattgt tggtgcagat ataccaatgt
      481 ttatcggagc aatgataatg ggaccatttg caggatgggt aattaagaag tttgataaat
      541 ttgtagatgg aaagatacca acaggatttg aaatgttagt aaataacttt tcaataggta
      601 taataggtat gcttcttgca atactaggat tttatgcaat tggaccagca atagtagcag
      661 ggacagctct tatagaatct ggagttcaat ttatagttag caaaagttta ttgcctctag
      721 tttcagtatt tatagaacct ggaaaagtat tattcttaaa caatgcaata aatcatggta
      781 tattagggcc tataggtata gctgaggcaa aagaagcagg taaatctata atgttcttac
      841 tagaaagtaa ccctggacca ggtttaggag ttctattagc atattggatg tttagtagag
      901 gaagtgtaaa acaatcagca ccaggagctg taataataca tttctttggt ggtatccatg
      961 aaatttactt cccatatatt ttaatgaatc cagttttaat attggctaca atggcaggtg
     1021 gagcagcagg aatattgact ttttcaatat taggagctgg gcttgtagca gcaccatcac
     1081 caggaagtat atttgcactt atggcactag ctccaaaggg aggattactt ccagtattag
     1141 caggagttgc agtggcaaca gttgtatcat tcttagtggc agcaccattt gttaagagag
     1201 cttcagcaaa tcaaagtgaa gaagacagta cttctttaga agaagctaaa gcaaaaatga
     1261 gtgatatgaa atctgcaagt aagaactctg agaaaaatat tgaggaaaaa cagcttgaag
     1321 taaatgagat taagaagatt gtatttgcat gtgatgcagg aatgggttcg agtgcaatgg
     1381 gagcttccag atttaaaaat agaataaaaa acttagactt aaatatagaa attacaaatt
     1441 catctgttga taatcttcca gatgatacac aaatagtagt aactcacaat acattagttg
     1501 aaagagttgc taagaataat tcaagtgttg agatagtttc aataaacaat tttttaaatg
     1561 acccaaattt agatgcatta tttaaaagat tagaatctaa ataaaaataa aaagtaccac
     1621 taattaggag tggataattt gaaaaaaaag aagattacat ctaggcaaaa aaagataata
     1681 ctaatgatag tagaaaattc aaagaaaaac attccaataa ctatttctga gattgcaggg
     1741 actttagagc taagttcaag aactgtactt agagatatgt ctggaattga aaaatggttt
     1801 gatgaaaatg attttaactt tgtaaaaaaa ccaggagtgg gattgatttt agaggaaaat
     1861 atagaaaatc aaaactttat aattgagttg ttagaagaag aaaaaataga aaaagaatac
     1921 agcaaggaag aaagaaattt aattatatta tcaaagctac tagtttctaa cgagccagta
     1981 aaatcctatt attttgcaaa aatattaaag gtatctgaag gggtactgaa taatgatttt
     2041 gccttagcaa gtaaatggtt ggaaagattt gatattgagc ttgttagaaa accagggctt
     2101 ggtgtctact taaaaggtca agaaaaaaac tttagagagg catatgtaaa tcttatatac
     2161 gattcattca atgaaaaaga aatactagat atggtcagaa atataagtga aaatatacaa
     2221 acagataaag caatagaaat tttaagtgaa aatagacttt taaatttaat ggacaggtgc
     2281 ataataagaa aagttgaagc tacattaaca aagaagcttt cagatttaga tgtaaattta
     2341 gctgatagtg catatatagg gcttgtagtt catatttcat tagccttaca gaggataaaa
     2401 aatggtgaaa atataactat ggataaagag tttctaaaag aattatctat aacagaagag
     2461 tttaaattag ctaaagaaat cgtaaaaggt atggaaacag attttaacat ggatatacca
     2521 gtagatgagg ttggatatat aactatgcat ataagaggtg caaagcaaag gtcatcttca
     2581 aaccataagg ctttaaactt agatgatata gaaataatgg aaattacaaa taagatgata
     2641 gatttggcgg aagatgaatt taaaataagt ttaaaaaatg acgagaggtt atttaaagac
     2701 ctagcaaatc acttagggcc ttctataaat agattgaata tgggattaga aataagaaat
     2761 cctcttttag atgaaataaa aagcaagtat tcatatgcct atgatggagt tgaaaagatt
     2821 tctagaatta taaaagataa actaaatata aattcaatac ctgaatcaga aataggatat
     2881 attgcaatgc actttgcatc agccatagaa aaaaatctta tgatgaatac taatataaat
     2941 attgttgtgg catgtcccac tggaataggt acttcaagat tcttatcaac taagattgaa
     3001 aataaatttc ctaatttaaa tatacttgaa accatatcag ctataaacat agatgaagag
     3061 tatttaaaag aaaaagatgt tgatttaata gtgtctacag ttgagttaaa tacaagttta
     3121 aattatatat gtgtaggtcc atttatgagt ttagatgatg aacagataat caaggaaaaa
     3181 ataaaatcta tagctcaaaa taaattaatc aatttaaatg cgaaaaatga tactaaaagt
     3241 aagaataaag tatatgaaca gataacagaa agtatgaata taggaaaaga tattcttcaa
     3301 tttttagagg aaattagatt tgaaaaattt gaaagtaaag atttaagtga gcttattgaa
     3361 gactcatcaa aaatatttgc aaaatctagt gaagatataa tctctataaa agaatcgtta
     3421 aaagaaagat taaaaatctc aattccatat atagaagaat caaaaattct attgttacat
     3481 tgtatgagtg aaagaataga tattatgaag ttatcaataa taaaactaga gaatagaatc
     3541 gtgctagatt caaatgaaga aatcgataat gtagttttta tgttacttcc taaaaattca
     3601 ccaagttatc aaagacaaat aatgagtgaa ataagtggtt cattaataga caatataatt
     3661 tttactaata agataaataa attctctata gaagagatga cattagaggt aaaagatata
     3721 gtttttaatt tttatacaaa cagattaaaa gcatttatag ataaataaaa ttaatattaa
     3781 aaaggaaggt tgatactatg agtaaagtat tgaatgagaa taatatattt ttagggttag
     3841 atagtgtttc aaaggaagaa gctataactt tagcaggaag aaaattagtt gaaaatggat
     3901 atgtaaaaga agaatatata ccagcaatgt tagaaagaga aaaagtaatg actacatata
     3961 tgggaatggg agttgctatt ccacatggtg taaatgaagc aaaaaaagaa atattatctt
     4021 ctggaatagt tatattacaa ttccctaatg gtatagattt tgatggagaa aaagcatatt
     4081 tattaatagg aatagctggt gttggagatg aacatttaga aatactatct aatatagcag
     4141 tagttttaga tgatgactta acagaaagac taaaaaattc aaatgacaaa caagctttta
     4201 tggaagcttt tgccaactaa tcttaggagg gatttaaaat gaaaaaggcg attcagtttg
     4261 gagcaggaaa tattggaaga ggttttattg gaagcttgtt agttaagtcg ggttatcatg
     4321 tagtatttgc tgatgtaaat gaggagatat taaactcaat aaacaaagat aaaaagtata
     4381 ctatacacat aagagatgta gaatgtatag atgaagtaat tgataatata agtgcggttt
     4441 catctataaa agaagaaata atagatgaga ttgtacaggc tgaaataata actacagcag
     4501 ttggaccttt agttttgact aaaatagcct ctacaattgc aaaaggtata aaagctagaa
     4561 aagaaaaggg attaacaagt aatctaaata taatagcatg tgaaaatgca atatacgcaa
     4621 gttcttcttt aaaagaagag gtacttaagt atttaaataa ggaagaagta gaatatttag
     4681 agatgtatgt tggttttcca aactgttcag ttgatagaat agttcctcct ggtaaaaacg
     4741 aaaatcctct tgatgtaaca gttgaaaatt tttatgagtg gaatgttgaa aaacaaggat
     4801 ttaaagggga aataccaact atagttggaa tgaatttagc tgataacctt atggcttata
     4861 tagaacgtaa attgtttaca ctgaatacag gtcatgctat aacagcttat ataggatatt
     4921 taaaaggata taagactata gaagagagta taaaagataa atttatctgt gatatagtca
     4981 aaagtgcaat ggtagaaagt ggagaaggat tgattaaaaa atacaatttt gactcagaag
     5041 tgcactataa atatattgat aaaattttaa atagatttaa aaatccatat cttaatgatg
     5101 atgtattaag agtcggaagg gaaccattaa gaaaactaag tgataaagat agattgatta
     5161 aacctttaat gacagcaaaa tcatatggat tatcagttga caatttgatt cttggaatag
     5221 gtgcagcgct tcattataat aatagtgaag atactcaaag tgtagaatta caagagttga
     5281 taaagtctat tggggtaaaa aaagccgttg ctaaaatagc taatataagc aatgacgaag
     5341 aaattttaaa taatatcgaa aaaagttata tatttatgaa aaatttataa atttacttga
     5401 aaaaaagtag ttctgataaa gtaactataa tatattattc tacacagttt gaattatttg
     5461 ttgaataata gagaattata atatataatt aaaaggaata aattaatata tttacctgta
     5521 tatgtttgat aatatggttc aaatgtttct actaagctac cattaatagc ttgactacag
     5581 gagtgaagaa gatatagttt atacttattt taaattatat attaagttaa ttactttgaa
     5641 ttttaagctc ctgtctatga tatagacagg agctttttta gtattttaat ataaaatact
     5701 tacaagaata gatagcaaaa ttagaacaaa tataattttt ttattaatat aattttagaa
     5761 agggctaggt ggaagttcaa tgaaggcttt acaagaaaag atattgagag aaggcagtgt
     5821 ttcaggaaat gatatactaa aagttgatag ttttttaaac catcaaattg atgtagcttt
     5881 tttaaatgaa ataggaagag aatttaaaga gagatttaaa ggagaaaagg tggataagat
     5941 atttacaata gaggcttcag gaatagctat agcatcaatt gtttcacagt attttgataa
     6001 tgcacctgta gtatttgcaa aaaaatcaga atctaaaaat ttggataaag atgtatatga
     6061 aactaatgta tattcattta ctaaggcaag agagtattca gttaaagttt ctaaaaagta
     6121 cataaacaaa ggcgaaaata tattgatagt ggatgatttt ttggcaaatg gaagagctgc
     6181 tcttggactt aaagatttaa ttgagcaagc agaagctaac ttagttggag taggaattgt
     6241 cattgaaaaa ggatttcaag ctggaggagc acttttaaag gctaatgatg taagattaga
     6301 gtctttggca gtagttgaat ctatagataa tggaactgta aaatttaggt aatttatgat
     6361 attatataat tgtgtttagc acatatttta actatgtaat tatggaggat tttttatgag
     6421 gttttttata gacacagcca atattgaaga aataaaagaa gcaaatgatt taggtgtaat
     6481 atgtggtgta actactaatc cgtctctaat agctaaagaa ggtagagatt ttatagaggt
     6541 agttaaagaa ataagtgaaa tagtagatgg gccaataagc gcagaagtaa taagtttaga
     6601 acataaaggt atgatagagg aagcagaaaa attatctaag atacataaaa atatagttat
     6661 aaaaatacct atgacagcag aaggtctaaa agcggtgaaa gtactatcaa gtaaaggtat
     6721 aaagactaat gtaacgctta tattttctgc tggacaagct cttttagctg ctagagctgg
     6781 agctacatat gtaagtcctt ttgttggtag attggatgat atatcacaaa atggtcttga
     6841 tttaattgaa gaaatcgttg atatatttag tgtaaatagt attgaggctc aaataatagt
     6901 tgctagtgtt agaaatccaa tacatgtttt acaagctgct agaatgggag cagatatagc
     6961 aacagttcct cttaaagtta taaaccaaat gattaagcat cctttgacag acaaaggaat
     7021 agatagcttt atgaaggatt gggaaggtgc ttttttaaaa ttataattat aaactacgct
     7081 ttataagtat attaaagagg ttattatgga aattctaagt aataaggcta ggctattttg
     7141 cctagtctta ttttggtgta taatatattg aaacatatta ttgaaataga ttaatttttt
     7201 aagtgaggag gcgaaaaatg gataaaatat taagagagat acaaagagaa gataaaaaaa
     7261 atccatatac tgaccaagaa cttgctgaaa tgttaaatgt agctaggtct gaagtgatat
     7321 cagttaggaa aaaaaataat attttagatt cgagagaaag aagaaaaaaa atattaataa
     7381 aagatatatc aaaaattgta agtgaaaatc ctcaaatgtc tgaaagaaaa ataactgaaa
     7441 tattaataga aaatggatat aatatatcga gaagtgcagt ttcaaaatta ttaaaagagg
     7501 aaaatttaaa tcagtcaaag attgggatta aggataaaga cccagcatca tcaggaaagt
     7561 atgtgaaggt gagtaaagtt gtagaagaag atggatttga agaattgata ggtataaagg
     7621 gaagtttgaa agaaaaggta aatctagcta agtcagcaat aatgtatcct cctaatggcc
     7681 ttcatactat aatatatgga gaaacaggag taggtaaaag tgaattagct acatgtatgt
     7741 ataaatatgc tataaaaaac aacattaaag aagagaacag cccatttata gtgtttaact
     7801 gtgcagacta tgcagaaaat cctaatctat tgatagctca actctttggg gtagtaaaag
     7861 gtgcatatac aggagcagat gcaaatagag aaggtcttgt agaacaagct aataatggaa
     7921 tattgttttt agatgaaatt catagattgc ctgctgctgg gcaagaaatc ttattttcat
     7981 taatagatag aggagaattt agaaggttag gagaaagtag ttctaataga aaggcaaatg
     8041 ttctcataat aagtgctaca acagaaaatc cagattctaa tttacttcaa acatttagaa
     8101 gacgtatacc aatggtaata aatttaccta atatgagtga aagaccaaaa tctgaaagat
     8161 atgaaataat aataaagttt tttgaaagag aagcaaagcg agtaaacaag aattttataa
     8221 taactaaaga agttatgtgt gcacttatga attataagtg tactggaaat ataggtcagt
     8281 taaagagtga tatacaagta acttgtgcta gagcttttag taaatcgata ttttctagcg
     8341 ataaggtaat tatagattta gatagtttaa gggattatat aaaaagtgga tattatgatt
     8401 gtaattgtaa aaatgaaaag tattctgagt tttcagttga ggatatatat atagatatta
     8461 gtgaattggt gagtggaaaa aatagaattt tatctgataa tgagataagc gagatatata
     8521 attatgcaga aaaagagctt aaggttttgg aaagtaagtg cttttcagaa gaagagttaa
     8581 aaaatgcatt tatagaaaag ttagataaga agtttaatgc tattaaaaac aataaaaatt
     8641 tatctgatag aagaagtcga atagactggg gaggacaatt aaaagagtct acactggaga
     8701 taatggataa ggtaattgta tttattaagg agcagtttaa gcatgtaaat caaggtctgt
     8761 acttggctct agcaattcat attgagcatg ctataggtag gataaaagat ggaaaaacta
     8821 taataaatcc gagtttagat aaaataaaag catcaatgcc aatggaatat gagttgtcaa
     8881 gatatataat gggtatagta gaagatttga cacaagtaac atttccagaa gatgagcttg
     8941 gatatttggc ttattatata aataaatttt gttataatga ggaaagtata aaagataaag
     9001 ttaaagtagt aatagttacg catggtaaag ttggaattga aatgtctaaa gttgtaaatc
     9061 atattttagg tatagaatgt acactaggta tagaaatagc acttacagat tcaccatcag
     9121 aggggataga gcatgtatta gaagagcttc aaaagataga agctagaaaa ggtattctag
     9181 ttctaattga tatgggttct ttggtaattc ttggtgatga ggttgaaaaa aggcttggaa
     9241 ttagatgtaa aactgtaaat agagtagata cattattggc aatggaagca ggaaaacttg
     9301 caaccattga aggaaaaagt ttagatggta taatagctga tttaaagaaa aataagaatt
     9361 atgctatgat aaatacaaat aaatttagtt acagaaaaaa cgagtatgga aagaaaaatg
     9421 taatcattac tttatgtttg agtggtgtag gtacagcttt aaatttaaaa gagcatatag
     9481 aaaaacaaat taaggaatat ggcactttaa ttgaagtaaa gccagttgct tttttaaata
     9541 ataatttaga agaagagtta aatgatattg aagctgaata taatattatt gctatctgtg
     9601 gaactattga tatagattat aaaaacgtgc cttttatttc ttataatgag gtacttggta
     9661 aaaatggtat taataaagta ttagagcatc taaatagtaa acaaccagct ttaaatattg
     9721 aaaaccagtt gaataattta atacatgaag atttgatttt atacaatttt gaaggaattt
     9781 caaaggagta tataattgat actcttgttt caaaacttga agaaggtgga tatgttgatt
     9841 caaagtatat attaagtgta tataaaagag aagcaatggg aagtgttgta atggcctcta
     9901 aggtagccgt gccacatgga cttccagaaa acgttataaa gcctgcaata gcaatcgcaa
     9961 ggttgaataa acctatagta tgggacaata aatttatggt ggatttggtg gtactactgg
    10021 cacttaaaga aaataataag aaagaaatac gaagcttatt ttcaaagatt aatgaccagc
    10081 atactttaga agttttatta aatactgaag ataaaaatga aataaaagaa cttttaagct
    10141 aattattagt accgagaaga aagacaaaaa aatttgcttt agtataaaat ttaaaattac
    10201 tcaattgcta agtagttaat tactc
//
LOCUS       DAMJWP010000121        10209 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.126, whole genome shotgun sequence.
ACCESSION   DAMJWP010000121 DAMJWP010000000
VERSION     DAMJWP010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10209
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.126"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            109..2298
                     /locus_tag="PC700_002418"
     CDS             109..2298
                     /locus_tag="PC700_002418"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HDF2581720.1"
                     /translation="MKYIIKRDGTKELFSKSKIEDAVYKASINSEGGVDRELAYDISR
                     KIANDYNSMEKCLSVEEIQDIVEVLLMESNRKDIAKHYILYRERRSEIRTKAWEFDEL
                     QKSIWKNKYRHNGESFDEWIKRISNNSSRISKLIRQKRFLFAGRILANRGLQKEGKKV
                     TYSNCYVIEPPKDSIEDIFDTAKKLARTFSYGGGVGIDISKLRPKGAKVNNAAKHTTG
                     AVSFMELYSMTTGLIGQRGRRGALMISMDVNHPEIEEFIDIKTDLEKVTKANISIRVT
                     DEFMQAVREKKLYNCTFEVETTGEKIVKIVNAYDLFNKFVKNNWDYAEPGILFWDKIK
                     TYNFMSEDKNFCFSGVNPCAEEPLPSGGSCLLGSINLSEFIIQPFGEHATFDINKLKS
                     CVREAVVALNDVLDEGLNLHPLQEQRDSVHKYRQIGLGVMGIADMLIKMGIRYGSDES
                     LELCDLIGKTMLNEAVKQSALIAKEYGTFSEYKYENISKSKFFIENLNEDTKEMVKKY
                     GLRNSQLLTIPPTGSISTMIGISGGIEPIFNLSYIRKTESLHDEDVYYKVYTPIVKEY
                     MDKKGITDEECLPDFFVTAMNIGYEERINMQKVWQKYIDASISSTINIPFETTVEDVF
                     NIYIKAWEAGLKGVTIFRDGCKRSGILINEKKEENNKEDKKENSKKEYYKNNKKEKDN
                     KDEKAKEIINKNFEEDEKFVCPECGSEGIAHTGGCSICLKCGYSGCN"
     gene            2366..3133
                     /locus_tag="PC700_002419"
     CDS             2366..3133
                     /locus_tag="PC700_002419"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDF2581721.1"
                     /translation="MQDKSVREIKEIIETLEVEKYMEYIELLRVDERKSVQGLAIKLA
                     KKLDNIRKEEERLETINIFENEGYDKGYLYIGGIDEAGRGPLAGPVVASVVVFKKDTK
                     IEGVNDSKKLSEAKRDELFEVIKEEALDYGIGIVNNEEIDEFNILNATYMAMKKAINC
                     LKKAPDYLLVDAATIPGIDISQNPIVKGDSKSISIAAASILAKVTRDSIMYQYDRVYP
                     EYGFKSHKGYGTKEHYEAIEKYGITPIHRKSFLKNIL"
     gene            3191..4207
                     /locus_tag="PC700_002420"
     CDS             3191..4207
                     /locus_tag="PC700_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDF2581722.1"
                     /translation="MEYNELLKSARENLNGSCKVCKVCNGVACAGEVPGMGGKGSGSS
                     FIENSKSLEKVKVNMRVIHDVSNPDTSIEMFGKKMSAPIFAAPVTGTTLNMGGKINER
                     DYIEPVVAGCANSGIYAMVGDTAVDAFLMENLDVVKKYNGAGIVFIKPWDNENIIKKI
                     RLAEEAGAFAVGVDIDACGLVTLSLHGKPVLPKNVKQIKELVKSTKLPFILKGIMTVE
                     DALMAVEAGVYAIVVSNHGGRVLDCTPGACEVLPKIADAVKGKVTILADGGVRTGLDV
                     LKMIGLGADAVLIGRPFVTASFGGATDGVETYVNKLQSELSSSMILTGCQTIKDIDGK
                     VIYK"
     gene            4273..4521
                     /locus_tag="PC700_002421"
     CDS             4273..4521
                     /locus_tag="PC700_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581723.1"
                     /translation="MSRKAYEEALVELEKFIDERKEIIKSAEDCIDKYIVDRTLPFDY
                     KDKCVEWQQELLDIAEAQVLEANELGVLLEEKKELEEE"
     gene            complement(4698..5195)
                     /locus_tag="PC700_002422"
     CDS             complement(4698..5195)
                     /locus_tag="PC700_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HDF2581724.1"
                     /translation="MYKIGESVMYPKEGACYISGLVTKDINHHIQKYYELTVIYNSNL
                     KISIPVLNADKIGVRPIMDENEVENFIQSLDKVDCLWVFDRKKRLKLYHDKFHSGDVF
                     EIVKLIKMLMIQDNSKQLCSTDKDFLNKAQRFALSELAAAQCKSYTIVLEEMKNHILN
                     SKNNN"
     gene            complement(5436..6500)
                     /locus_tag="PC700_002423"
     CDS             complement(5436..6500)
                     /locus_tag="PC700_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2581725.1"
                     /translation="MIRLTIANIKRYIKNHALLVNMVLFPVILMFYLNFFTGNSENQS
                     MYFSPVAIVSNSSGKYEHKLIDSSKLKENSFGLNEQDKAMDLLKNNKVSAVFILDKNF
                     SSNIDNLKRPEVKCFKTENGGGSLWAESQIESFITNSLKLKIDRNIDDKLTKTNIIDN
                     SSGKNKNSFLIVFLICYFMYINAAHLASDLFSLKKSNTLKRLVSTKHNDIKIIFSIFL
                     GLFFTQAIVYTLTLVCFTSISNFKLTLNTLMIVLANSFVATGFVFWIARVFKNEGSIS
                     LMSTFYSLIGLAISISNMIPSLDKLSFMANLSKLTPFYWTIDAINNNGNILINIIVLI
                     LIGMVFVTAGSLNLRDFARN"
     gene            complement(6503..7600)
                     /locus_tag="PC700_002424"
     CDS             complement(6503..7600)
                     /locus_tag="PC700_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2581726.1"
                     /translation="MKLYISVKTMLKVLKSSFMLNLIYFLALPLVLAGFLGMVTESIF
                     QNPIKTDLTSIVIYDKDNTKLSSNLTGYLKNDLSSILTVQKDDSKADLKLTIPKGYEN
                     NLLNEKSNNLSIEKLKSNDNIAILLQDILDTYHEKLYLNNSQKLSSEDFSKLFNKNSI
                     DTSIIKNNVKQNSYEYFALVSLGFFAIIFIMNSILSNYVSESKGLSKRLYSIPVTRVQ
                     FLIYDFIGFWIYSFIFLSLYVLFFRTLGVTFKGNFVILALLCALASYFMTSISTFITT
                     FFSKKYGTVIVYALLFLQTVFGGIFSIVNDVFTKFTSLSPTYLIGTLFSNYETFKTID
                     SIESLIFTCLIASTVLIALSIVKEKYKWREV"
     gene            complement(7614..8549)
                     /locus_tag="PC700_002425"
     CDS             complement(7614..8549)
                     /locus_tag="PC700_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2581727.1"
                     /translation="MDIVRVNNITKRFNDKLALDNISFSVKEGEIFGLIGPNGAGKST
                     LINIITNLMLPNSGDIQINGLDLSKDCIKAKSIIGLVPQELALIETLTPFDNLEYFGT
                     YYGLKGKLLQERITEALEVTGLTDVKKKKIKKLSGGMQRRLNIGIALLNHPKILILDE
                     PTVGVDPQSRNHIFNFIKNISKKQKTTVIYTSHYMEEVEHLCSKIFIMDEGKEIAFGD
                     NDYLKSLVSTNTKLIMEIKNINAQLIFDLKNTKGVVSILENNSLLELGVDKKLQLTDV
                     LSVVDKNNSKITRISYEEPSLEDVFLNLTGKNLRD"
     gene            complement(8737..9381)
                     /locus_tag="PC700_002426"
     CDS             complement(8737..9381)
                     /locus_tag="PC700_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2581728.1"
                     /translation="MNKIKVLIVDDEKLIRKGLNIILSSYDDLEIVGDASNGYEALEF
                     CKTNDVDIVLMDIRMKVCDGVLGTRLIKEYDSSIALLILTTFNDDEYIEDAMKFGASG
                     YLLKDSSDEVLHEGIRSSFFGNIVLDKSVAEKIMYPQKTIEQKHISDMYNLTEKEISI
                     IRLIANGLNNKEISQELFLSEGTIKNNITNILGKLELRDRTQLVIFAFKNKIVI"
     gene            complement(9374..>10209)
                     /locus_tag="PC700_002427"
     CDS             complement(9374..>10209)
                     /locus_tag="PC700_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2581729.1"
                     /translation="IFSFIIGLDKNLYLALESSSTLLIITLFSVYLENENLSKLYNQS
                     LYDKLRVSKDELKKANADLEIYANSIEELAILKERNRISREIHDSVGHSLSTTIIQLN
                     AIEKLLKDKPLISDLVHELREFVTESFQDVRRAISELKPIEYENYQNLLKIRELVNSF
                     IKLTNINVRLTISKNTWNLSTNQSIALYRLVQESLSNASRHGKATEIRIFITFNPSNL
                     IITISDNGIGCNNIKRGNGLSSISERIYELNGKVEFDNSNTGFTIRASFPKFSGGDFF
                     E"
BASE COUNT         3877 a         1215 c         1620 g         3497 t
ORIGIN      
        1 attatgttag ataattaata tgaaaatata caaatatata aaaaagtttg tttagagtta
       61 tagttaatgt gtgtattaat ttaatactaa tttattggag gtttaaaaat gaaatacata
      121 ataaaaagag atggtacaaa agaacttttt agtaaaagta agattgaaga tgctgtctat
      181 aaagctagta taaatagtga aggtggagta gatagagagt tggcatatga tatatcaaga
      241 aaaatagcta atgactataa ttctatggaa aaatgcttat ccgtagaaga aatacaagat
      301 atagttgaag tactacttat ggaatcaaat agaaaagata tagcaaaaca ctacatatta
      361 tatagagaaa gacgctctga aataaggact aaagcttggg agtttgatga attacaaaaa
      421 tcaatatgga agaacaagta tagacataat ggtgagagtt ttgatgaatg gataaagaga
      481 atttcaaata atagttcaag aatatcaaaa ctaataagac aaaaaagatt tctatttgct
      541 ggaagaattt tagcaaatag aggattacag aaagaaggaa aaaaagtaac atattctaat
      601 tgttatgtaa tagaaccacc aaaagatagc atagaagata tatttgatac tgcaaaaaaa
      661 cttgctagaa cttttagtta tggtggagga gttggaattg atatttcaaa gctaagacca
      721 aaaggagcga aagttaacaa tgctgcaaaa catacgactg gagcagtctc ttttatggaa
      781 ctttacagta tgacaactgg cttaattggt caaagaggga gaagaggagc tcttatgatt
      841 tctatggatg taaatcatcc ggagatagaa gagtttatag atataaaaac agatttggag
      901 aaggttacta aagcaaatat ttccataagg gtaacagatg aatttatgca agctgtaagg
      961 gaaaaaaagt tatataattg tacttttgaa gttgaaacta ctggagaaaa aatagttaag
     1021 atagtaaatg cttatgattt atttaataaa tttgtgaaaa ataactggga ttatgcagaa
     1081 ccaggtatat tgttttggga taaaataaaa acatataatt ttatgagtga agataagaac
     1141 ttttgctttt caggagtaaa tccttgtgca gaagaaccac ttccaagtgg aggaagttgt
     1201 ctacttggct caataaactt gtcagagttt ataatacaac catttggtga acatgctact
     1261 tttgatatta ataaattaaa atcttgcgtt agagaagctg tagtagcttt aaatgatgtc
     1321 ttagatgagg ggttgaactt gcatccatta caagaacaaa gagatagtgt acataagtat
     1381 agacagatag gtttaggtgt aatgggtata gctgatatgc ttataaaaat gggaataaga
     1441 tatggttctg atgaatcttt agaactatgt gatttaattg gtaagactat gttaaatgaa
     1501 gctgtgaagc aatcagcatt aattgctaaa gaatatggaa catttagtga gtataaatat
     1561 gaaaatattt ctaaatctaa gttttttata gaaaatctaa atgaagacac taaagaaatg
     1621 gttaaaaagt atgggcttag aaattcacaa cttttaacca tacctcctac aggttctata
     1681 tctacaatga taggtatcag tggtggaata gaacctatat ttaatctttc atatataaga
     1741 aaaactgaaa gcttgcatga tgaggatgtg tattataaag tatatactcc aattgttaaa
     1801 gagtatatgg acaagaaagg tattacagat gaagaatgtt taccagattt ttttgttact
     1861 gctatgaata taggatatga agaaagaata aacatgcaaa aagtatggca aaagtatatt
     1921 gatgcttcta tatcatctac aataaatata ccttttgaaa ctacagttga agatgttttc
     1981 aatatatata ttaaggcatg ggaggctggt cttaaaggag taacaatatt tagagatgga
     2041 tgtaaaagga gtggaatttt aataaatgaa aaaaaggaag aaaataataa agaagataaa
     2101 aaagaaaata gtaaaaaaga atattataaa aataataaaa aagaaaaaga taataaagat
     2161 gaaaaagcca aagaaataat aaataaaaat tttgaagaag atgaaaaatt tgtgtgccct
     2221 gaatgtggaa gtgaaggaat tgcacataca ggtggatgta gtatatgttt aaaatgtggc
     2281 tatagtggat gcaattaaac tctaattaaa aaattttaat atatttaaaa attaaatttt
     2341 taaaaacgaa agatgaggga atggtatgca ggataaaagt gtgagggaga ttaaagaaat
     2401 aatagagact ttagaagtag aaaaatatat ggaatatata gaactgttaa gagttgatga
     2461 aagaaaatct gtacaaggtc tagctataaa actagcaaaa aagctggata atattagaaa
     2521 agaagaagaa agattggaaa ctataaatat atttgagaac gaaggctatg ataaaggtta
     2581 tttgtatatt ggaggaattg atgaggcggg aagagggcca cttgctggac ctgttgtagc
     2641 ttctgttgta gtatttaaaa aagatacaaa aatagaaggt gtaaatgact ctaagaaact
     2701 gagtgaggca aaaagagatg aactttttga agtaataaaa gaagaagcac tagattatgg
     2761 aataggtata gtaaataatg aagaaataga tgaatttaat atattaaatg ctacatatat
     2821 ggcaatgaaa aaggctataa attgtttaaa aaaagcaccg gattatttat tagttgatgc
     2881 agcaacaata ccaggtattg acatatctca aaatccaatt gtgaaaggag attcaaaatc
     2941 tatatcaata gcagcagcaa gtatacttgc taaagttact agggatagta taatgtacca
     3001 atatgataga gtatacccag aatatgggtt taaatctcac aaaggatatg gaactaaaga
     3061 acactatgaa gctattgaaa aatatggcat aactcctatc cacagaaaaa gttttttgaa
     3121 aaatatactg taaatataat atgatataat gtatataata taaattacta tagttaatgg
     3181 gggattaaag atggaatata atgaattatt aaaaagtgct agagaaaatt taaatggaag
     3241 ttgtaaagtt tgtaaggttt gtaatggggt tgcttgtgca ggtgaagtgc ctggcatggg
     3301 aggtaaagga agtggttctt cttttataga aaatagcaag agtttagaaa aagtaaaagt
     3361 aaatatgaga gtaatacatg atgtatcaaa tcctgataca tctatagaga tgtttggaaa
     3421 gaaaatgagt gcacctatat ttgcagctcc agtaacagga actactttaa atatgggtgg
     3481 aaaaattaat gaaagagatt acatagaacc agtagttgca ggatgtgcta atagtggtat
     3541 ttatgcaatg gttggtgata cggcagtaga tgctttttta atggaaaatt tagatgtagt
     3601 caaaaagtat aatggagctg gaattgtatt tattaaacct tgggataatg aaaatattat
     3661 aaaaaagata agattagctg aagaagctgg tgcatttgca gttggagttg atatagatgc
     3721 ctgtggtcta gtaactttat cattacatgg aaaaccagta cttcctaaaa atgttaaaca
     3781 aataaaggag ttagtaaagt ctactaaact accatttata ttgaaaggta taatgactgt
     3841 tgaagatgct ttaatggcag tagaagcagg tgtgtatgca atagttgtat caaatcatgg
     3901 aggaagagtg cttgactgta caccaggagc atgtgaagtt ttaccaaaaa ttgctgatgc
     3961 tgttaaaggt aaagttacaa tacttgcaga tggtggagtg agaactggtt tagacgtact
     4021 aaaaatgata ggtttaggtg cagatgcagt attaatagga agaccttttg tgactgcttc
     4081 atttggtggt gcaactgatg gtgtagaaac ttatgtaaat aaacttcaat cagagctaag
     4141 tagttctatg atattaactg gatgtcaaac tataaaagat attgatggaa aagttatata
     4201 caaataaaat tttatacaaa aaagaggaaa aatcatttta atgtagaata attgtacaaa
     4261 gggggaattt atatgagtag aaaagcttat gaagaagcct tagtagaatt agaaaaattt
     4321 attgatgaaa gaaaagaaat aattaaaagt gcggaggact gtattgataa gtacatagtt
     4381 gacagaacgc taccttttga ttacaaagat aaatgtgtgg aatggcaaca agagttactt
     4441 gatattgcag aggcacaagt tttagaagca aacgaattag gtgttttact ggaagagaaa
     4501 aaagaacttg aggaagagta agttttatta ctaaatctag ggaaaatcaa gaatatgatt
     4561 tacccctaga tttttttatt taaataaaga tgtctatata attaatctat ctttatcaat
     4621 gttagattaa aaggtttgaa gaaaaataga gtaattgtat gaatagttaa acaattaaac
     4681 tagtctataa gaccaattta attgttattt tttgaattta gtatatggtt tttcatttct
     4741 tctaatacaa ttgtgtagga tttgcattgt gctgcagcca attctgacaa agcaaacctt
     4801 tgagccttat ttagaaagtc cttatctgtg ctacatagtt gttttgaatt atcttgtatc
     4861 ataagcatct taattaattt tacaatctca aaaacatctc ctgaatgaaa tttatcatgg
     4921 tatagtttaa gtcgtttttt tctatcaaaa acccataaac aatcaacctt atcaagggat
     4981 tggataaagt tttcaacttc attttcatcc ataattggac gtacaccaat cttatcagca
     5041 tttaaaactg gaatcgaaat tttaagatta ctattgtaaa taacagtcaa ttcatagtat
     5101 ttctgtatat gatgatttat atcctttgtt acaagaccac ttatataaca ggctccttct
     5161 tttggataca ttacactttc accgatttta tacatatggt tatacctcca aactgaattt
     5221 tagatattgc aatttgggta caatgcttta attatctaat aattaatatt atatcacgaa
     5281 atgcttgaca agtcaaaaag attaatatat aataaaaagt ttagaataat agatatatat
     5341 aattggctat aaaaatataa gataagagga tttaatatat taaaatatct agttagtatt
     5401 gtataaaata acatactaac tagatttaat aattattaat ttctagcaaa atctcttaag
     5461 ttaagacttc cggctgttac aaataccatt ccaataagaa ttaaaacaat tatatttatt
     5521 aatatattac cattattatt aatagcatct atagtccaat aaaatggtgt aagctttgaa
     5581 agattagcca taaaggataa tttatcaagt gatggtatca tattagatat ggatatagct
     5641 aaacctatta aagaataaaa cgtagacata agacttatag aaccttcgtt tttaaacacc
     5701 ctagctatcc aaaatacaaa tccagtagca acaaaactat ttgctagtac aatcatcaat
     5761 gtatttagag tcaatttaaa attacttata gatgtgaaac atactaaagt taaggtgtat
     5821 acaattgctt gagtaaaaaa taaccctaaa aatatgctga aaattatttt tatatcatta
     5881 tgctttgtag aaacaagtct ttttaaggta ttagatttct ttaatgaaaa taaatcagaa
     5941 gctagatgtg ctgcattaat gtacatgaaa taacatatca agaaaactat taaaaatgaa
     6001 tttttattct tacctgaact gttatcaatt atatttgtct tggtcaattt atcatctata
     6061 tttctatcta tttttaattt taatgaatta gtaataaaag attctatttg tgattctgcc
     6121 caaagactac ctccaccatt ttcagtttta aaacatttaa cctcaggtcg ttttaagtta
     6181 tctatattgc ttgaaaagtt tttatctaaa atgaagacag cagaaacttt attatttttt
     6241 aatagatcca tagccttgtc ttgttcgttt aatccaaatg agttttcttt taatttagaa
     6301 ctatctatta acttatgttc gtattttcca gaagaattgg atacaatcgc tactggagaa
     6361 aagtacattg attgattttc tgagttgcca gtaaaaaagt taagataaaa cattaatata
     6421 acaggaaaca aaaccatatt aactaaaaga gcatgatttt ttatatatct ttttatgttt
     6481 gcaatagtca acctaatcat tattaaacct ccctccattt atatttttct tttacaatag
     6541 aaagtgcaat caatactgta gatgcaatta ggcatgtaaa aattaaactt tctattgaat
     6601 ctatagtttt aaacgtctca taattactga acaatgttcc tattaaataa gtaggagata
     6661 aacttgtaaa cttagtaaac acatcattta ctatgctgaa aatacctcca aaaactgttt
     6721 gcaagaagag taatgcataa actataactg taccatattt cttgctaaaa aaggtagtta
     6781 taaaagtaga tattgaagtc ataaaataag aagctaaagc acaaagtaaa gctaatataa
     6841 caaagttacc tttgaatgtt acaccgagtg ttctaaaaaa taaaacgtat aatgataaaa
     6901 atataaatga gtaaatccag aaacctataa aatcataaat taaaaattgt actctagtta
     6961 caggtataga gtagagtctt tttgaaagtc cttttgattc acttacataa tttgataaaa
     7021 tgctattcat tataaaaatt attgcgaaga atccaaggga aactaaagca aagtactcat
     7081 aagagttttg tttaacatta ttttttatta tagaggtatc aatagaattc ttattaaata
     7141 attttgagaa atcttctgaa cttaattttt gagaattatt cagatataat ttttcatgat
     7201 aagtatctaa tatatcttga agtaatattg caatattatc attagattta agtttttcta
     7261 tacttagatt atttgatttt tcatttaata aattattctc atatccttta ggtatagtaa
     7321 gttttaagtc tgccttagaa tcatcttttt gaactgttaa tatagaagat aaatcgtttt
     7381 ttagatatcc tgtcaaatta cttgatagct ttgtattatc tttatcatat ataacaattg
     7441 atgtcaaatc tgtttttata ggattttgaa atatactttc tgtcaccatc ccaagaaatc
     7501 cagctaaaac tagaggtaag gctaagaaat atattaaatt aagcataaag gagcttttta
     7561 aaacttttag catagttttt acactaatgt acaatttcat gaaatcacct ccattagtcc
     7621 cttaaatttt tacctgttag atttaaaaat acgtcttcta aactaggttc ttcataagat
     7681 attcgtgtta tttttgaatt atttttgtca acaacagata atacatctgt aagttgtaat
     7741 tttttgtcta cacctaattc taataatgaa ttattttcta aaatagatac aacaccttta
     7801 gtatttttta aatcaaatat aagttgagca tttatatttt ttatttccat aattaatttt
     7861 gtatttgtag atactaatga ttttaaataa tcattatctc caaaagcaat ctctttacct
     7921 tcatccataa taaaaatctt agaacaaaga tgctcaactt cttccatata atgagaagta
     7981 tatattactg tggttttttg ctttttagat atatttttta taaaattgaa aatatggttt
     8041 cttgattgcg ggtcaacacc tacagttggt tcatccaata ttaatatttt aggatgattt
     8101 aaaagtgcta ttccaatgtt caatctcctt tgcataccac cagataattt tttgattttt
     8161 ttctttttaa catcagtgag tcctgtgact tctaaggctt cagttattct ctcttgaagc
     8221 aattttccct ttagtccata gtaagtgcca aagtactcta agttatcaaa aggagtaaga
     8281 gtctctataa gtgctaattc ttgtggaact aatcctatta tagactttgc ttttatacaa
     8341 tcttttgaca aatctaaacc attgatttgg atgtcgccac tatttggtaa cataagattt
     8401 gtaattatat ttataagtgt agatttacca gcaccattcg gtccaataag tccaaatatc
     8461 tctccttctt taacagaaaa gctgatatta tcaagtgcta atttatcatt aaatcttttt
     8521 gttatattat ttactcttac tatatccata ataattcctc ccaaaataat tttgtataag
     8581 ttaaaaatct ataatactat aataaagaat tatgaatcat aattctatta ctataagtca
     8641 ctaaaaaaag tgacttaagt cactttatat tttcatttat tataaatgag tttgtgagta
     8701 agaaatttct agggtataga tttcttacct atatatttat attacaattt tatttttgaa
     8761 ggcaaatata actagttgtg tcctatcacg aagctctagt ttccctaaga tattggtgat
     8821 attattcttt attgtacctt cagataagaa taattcttgg ctgatttctt tgttgttaag
     8881 tccatttgcg attaatctaa ttatggatat ttctttttct gttaaattat acatatcaga
     8941 aatatgtttt tgttctatag ttttttgagg gtacataatt ttttcagcca cacttttatc
     9001 aagtacaata tttccaaaaa acgaggaacg aatcccttca tgtaatactt catccgaact
     9061 atcctttaaa agatatcctg aagccccaaa tttcatagca tcttctatgt actcatcatc
     9121 attaaaggtt gtcaaaatta ataatgctat agagctatca tattctttta taagtcttgt
     9181 gcctagaact ccatcacaaa ctttcatccg aatatccata agaactatgt ctacatcatt
     9241 tgttttacaa aattctaatg cttcatatcc attggaagca tctcctacaa tttctaaatc
     9301 gtcataagat gataaaataa tgttaagacc ttttctaatc aatttttcat catctactat
     9361 aagaactttg attttattca aaaaaatcac ctcctgaaaa tttaggaaaa gacgctctaa
     9421 ttgtgaatcc agtattagaa ttatcgaatt caactttacc atttaattca tatattcttt
     9481 cgctaatact gctaagtcca tttcctcttt ttatattgtt acaccctata ccattatcgc
     9541 tgatagtaat tataagattt gaaggattaa atgttataaa aattctgatt tcagttgctt
     9601 ttccatgtct tgatgcgttg gaaagagatt cctgaacaag tctatataaa gcgatagatt
     9661 gatttgtaga taggttccag gtattttttg atatagttaa tctaacgttg atatttgtaa
     9721 gtttaataaa gctattaacc agttctctga ttttaagtaa attttggtaa ttttcatatt
     9781 caatgggttt tagttcagat atagctcttc tcacatcttg aaaactttca gttacaaact
     9841 ctctaagttc atgaactaaa tcagaaatga gaggtttgtc ttttaagagt ttttctatag
     9901 catttaattg gattatcgtc gtagaaagag aatgtcccac actatcgtgt atttccctcg
     9961 atatacggtt tctttctttc aaaattgcta actcttctat agaatttgca taaatctcta
    10021 aatcggcatt agcttttttt aattcatctt tagaaaccct gagtttgtca taaagacttt
    10081 gattgtataa tttagataaa ttctcgtttt ctagatacac agaaaagagg gttataatta
    10141 gtaatgtaga acttgattct agtgctaaat ataaattttt atctaaacca ataataaaag
    10201 aaaatatta
//
LOCUS       DAMJWP010000122        10121 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.127, whole genome shotgun sequence.
ACCESSION   DAMJWP010000122 DAMJWP010000000
VERSION     DAMJWP010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10121
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.127"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            58..1125
                     /locus_tag="PC700_002428"
     CDS             58..1125
                     /locus_tag="PC700_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HDF2581730.1"
                     /translation="MKNKLVYICIMSLLLFVVGCSNNPNKESTIKKDSKNNQSQVKEE
                     KREPVKKDITISFAGDVTMGNYKGSSYYGSFDHEFKRQGEDYNYFLKNVKPVFEKDDL
                     TVVNLEGPLTTASNAKEKKFAFKGKPGYVNILKSGSIEAVSIANNHSEDYFEEGMKDT
                     QFILDENKINYFGLGKDAVVDVKGIKVGLLGYNGLSTEYNEKNLKQMEDDIKSLKKKS
                     DVVVVYFHWGIELDYYPDKKQKDFAHYAIDKGADLVMGSHPHVIQGIEKYKNKYIAYS
                     LGNFCFGGNKNPSDTDSYIYQQTFTFSDNKLTSIKEPNIIPTSITSSSSRNNYQPKVL
                     DGSEKDRVLNKLEKISKDLNK"
     gene            1295..3196
                     /gene="cooS"
                     /locus_tag="PC700_002429"
     CDS             1295..3196
                     /gene="cooS"
                     /locus_tag="PC700_002429"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HDF2581731.1"
                     /translation="MACNTCKMCESADKKLESFVASKDVETAFHRTEDQKVKCGFGLQ
                     GVCCRLCSNGPCRVTPKSPRGICGADADTIVARNFLRAVASGAACYLHVVENTAKNLK
                     NVGITKGVVKGEKTLNQLAEMFGIESDEKYDKCIKVADKVINDLYKSRDDKMELVEKI
                     AYAPRVKKWKELGIMPGGAKSEVFDAIVKSSTNLNSDPVDMLVNCLNLGISTGLYGLT
                     LTNLLNDVMLGEPVIRMAPVGFNVIDPDYINIMITGHQHSTFANFQDRLKDEDVIKLA
                     QSVGAKGFKLVGCTCVGQDLQLRGEHYQEVFAGHAGNNFTSEAVLSTGAIDIVLSEFN
                     CTIPGLEPIADKYKVKMVCLDDVAKKANAEYIGLDRSKLDELSNTLIEKALESYKERR
                     GSIEIDIPKDHGFEQSLTGVSEKNLKAFLGDSWKPLINLIAEGKIKGVAAVVGCSNMT
                     AGGHDVNTVELTKELIKKDIIVLSAGCSTGGLENVGLMSPGAEELAGENLKEVCKTLG
                     IPPVLNFGPCLAIGRLEIVATELAAELGIDLPQLPLVLSAPQWLEEQALADGAFGLAL
                     GLPLHLALPPFITGGKLVTEVLTEKLKDLTGGHVIVNPDPKSSANQLEEIIIDRRKNL
                     GLKDVECNA"
     gene            3189..3662
                     /locus_tag="PC700_002430"
     CDS             3189..3662
                     /locus_tag="PC700_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2581732.1"
                     /translation="MHRIFINKDLCTGCKSCVLACMLKHNKDYDMYTLDLENIDNDSR
                     GHIELDSKHNNPVPILCRHCDEPECVLACMSGAMHKDSESGIVSYDEEKCGSCYMCVM
                     SCPYGLLKPDDRSKQNILKCDLCKDEEYPRCVANCPSGAIELQKEENDELCSVRG"
     gene            3637..4863
                     /locus_tag="PC700_002431"
     CDS             3637..4863
                     /locus_tag="PC700_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF2581733.1"
                     /translation="MNYVVLGASAAGINAVKTLRELDKDSNIVVISKDENVYSRCMLH
                     HVISEHRTLKQINFVDEDFMEQNNVKWIAGKTVKGIDINKKVVQTEDITVNYDKLLIA
                     TGASSAIPPIKNLRDGNFVYSVRNIDDIYKIKEKAQNSKNVVIIGAGLVGIDALVGLF
                     KYEQLNISVAFMEKYILDRQLDEYTASVYENKFKEKGVKFYPSASIQEIVLDNSKNVT
                     GVAFSNGEVLDADMVIVATGVKPNADFLDGTGIEYDRGIIIDDMCQTTQKDIYAAGDV
                     VGKNAIWPLAVKQGIVAAYNMVGKDKKIEDEFAFKNSMNFMDIPTISIGMNTPVDDSY
                     KVLTRHGINDYKKFVFKDNVIYGAVIQGDISYVGVLTYLIKNKVEIYDLENRIFDVCY
                     ADFFNIKENGEFCYSV"
     gene            complement(5002..6189)
                     /locus_tag="PC700_002432"
     CDS             complement(5002..6189)
                     /locus_tag="PC700_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2581734.1"
                     /translation="MNITKNFLKDYLEKFGETPFLVRLKDEEEFLIGEGSPQFKVIVN
                     NDISKSELLKSTSLALGEAYINRDIEVEGDLFLTLNLLLKQIDKFNIDNSAVKHLLHS
                     SNSMRHQKKEIHAHYDIGNDFYSLWLDKTLSYSCAYFRNDNDTLYDAQVNKVHHILTK
                     LNLEEGMSLLDIGCGWGFLLIEAAKKYKIHGVGITLSEEQFKEFNARIKEENLQDYLE
                     VRLMDYRELKKSDLMFDRVVSVGMLEHVGRENYELFIKNVNAVLKDGGLFLLHYISGL
                     QESYGDPWIKKYIFPGGVIPSLREMIHICSDYKYHTIDVESLRLHYVKTLLYWQKSFS
                     ENLPKIKEMFDDKFIRMWDLYLCSCAAAFNTGIIDIHQILFTKGIKNDLPLTREYMYN
                     CSK"
     gene            6462..7286
                     /locus_tag="PC700_002433"
     CDS             6462..7286
                     /locus_tag="PC700_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDF2581735.1"
                     /translation="MEVYALVGSSGTGKSFKALEFAYENDIEYIIDDGILIYKNKVLA
                     GISAKQANTTIEAVKRAIFYNLEHRQEVREAIKKENIRKILIIGTSKKMVNQIVERLS
                     IGRVCKFINIQDISTKYEIEIAKQARKEGNHVIPVPAVEIKSMASGLSINSLKRLFRK
                     GNNRNMVVLEKTIIRPTFSYIGKFYISADVIKQIIEYEVYNFGSIDRINKINIENCNN
                     FMNIFVSININDLETIKKIEDIQQTIKKSIEKMTLVNVQKIDINIHKIKKINFLQC"
     gene            7415..8680
                     /gene="gluD"
                     /locus_tag="PC700_002434"
     CDS             7415..8680
                     /gene="gluD"
                     /locus_tag="PC700_002434"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-specific glutamate dehydrogenase"
                     /protein_id="HDF2581736.1"
                     /translation="MSGKDVNVFEMAQSQVKNACDKLGMEPAVYELLKEPMRVIEVSI
                     PVKMDDGSIKTFKGFRSQHNDAVGPTKGGIRFHQNVSRDEVKALSIWMTFKCSVTGIP
                     YGGGKGGIIVDPSTLSQGELERLSRGYIDGIYKLIGEKVDVPAPDVNTNGQIMSWMVD
                     EYNKLTGQSSIGVITGKPVEFGGSLGRTAATGFGVAVTAREAAAKLGIDMKKAKIAVQ
                     GIGNVGSYTVLNCEKLGGTVVAMAEWCKSEGSYAIYNENGLDGQAMLDYMKEHGNLLN
                     FPGAKRISLEEFWASDVDIVIPAALENSITKEVAESIKAKLVCEAANGPTTPEADEVF
                     AERGIVLTPDILTNAGGVTVSYFEWVQNLYGYYWSEEEVEQKEEIAMVKAFESIWKIK
                     EEYNVTMREAAYMHSIKKVAEAMKLRGWY"
     gene            8873..9529
                     /locus_tag="PC700_002435"
     CDS             8873..9529
                     /locus_tag="PC700_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HDF2581737.1"
                     /translation="MYFLPPHDYKLEELDWIFGHELMYFKSKDLHLKYLVLFLKIVYW
                     FNPFIYIMEKYMDLDCELYCDERVLKNCTVQQKQEYALTIVNSMKRGINFSNKFVTGL
                     NKQSDIRKRVMNMFSEKHKNGILMALIISLFSSITFFKFDSTSLIEENDFLERSRYAF
                     EGRQEDMFVTIDSTYADAPIKLRKEHEENCRVNGIVPKDSDSIEIIPRFYKDCMDLKK
                     "
     gene            9860..>10121
                     /locus_tag="PC700_002436"
     CDS             9860..>10121
                     /locus_tag="PC700_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HDF2581738.1"
                     /translation="MSNFILNGIFRTVIVSSLSILLILIFKKNVFKRFSKKFNYYIWM
                     IVVIKLFLPFTYYTFTINILRSKKHININNINLEGFNNVSTIS"
BASE COUNT         3947 a         1095 c         1813 g         3266 t
ORIGIN      
        1 tatactctaa aaaagtatat tttattttaa aatttattaa tacaaaggag atttactatg
       61 aaaaataaat tagtttacat atgtattatg agtctattgc tatttgtagt aggatgttct
      121 aataatccaa ataaggaaag taccattaaa aaagactcta agaataacca aagccaagtt
      181 aaagaagaaa aaagagaacc tgtgaaaaaa gatataacta taagtttcgc tggtgatgta
      241 actatgggga actataaagg ttcatcatat tatggcagtt ttgaccatga atttaaaaga
      301 caaggagaag attacaatta ttttcttaaa aatgtaaaac ctgtatttga aaaagatgac
      361 ttaacagtgg ttaatttaga aggtccactt actacagcgt caaatgcaaa agaaaaaaaa
      421 tttgcattta aaggaaagcc tggatatgta aatattttaa aatctggaag tatagaggca
      481 gtaagtattg caaataatca ttcagaagat tattttgaag aaggaatgaa agatacacag
      541 tttatattgg atgagaataa aataaattat tttggattag ggaaagatgc tgttgtagat
      601 gtcaaaggaa ttaaagtggg tctattaggg tataatggat tatcaactga atataatgaa
      661 aaaaatttaa aacaaatgga agatgatatt aagtccttaa aaaagaaatc ggatgttgtt
      721 gtagtgtact ttcattgggg gatagaatta gattattatc cagataaaaa acaaaaagat
      781 tttgctcatt atgctataga caagggagca gatttagtta tggggtctca cccacatgtt
      841 attcaaggaa tagaaaagta taaaaataag tatatagctt atagtctagg gaatttttgc
      901 tttggaggaa ataagaatcc ttctgataca gatagttata tatatcaaca aacctttact
      961 ttttcagata ataaattaac ttcaattaaa gagccaaata taataccaac ctcaataaca
     1021 tctagtagct ccagaaataa ttatcaacca aaagtattgg atggaagtga gaaggataga
     1081 gttttaaata aacttgaaaa aataagcaaa gatttaaata aataaaatgt aaaaaaaaat
     1141 aaatatatat atttatttta ataatttata ttttgtttag ggtatggtaa atcgtggtat
     1201 taaataagta gattttaata gttaaattat agtcaattat aataaagcta tttaatctac
     1261 ttttttaata taaaaatata agggggaatt tgtaatggct tgtaacactt gtaaaatgtg
     1321 cgaaagtgca gacaagaaat tagaaagttt tgtagcttca aaggatgttg aaacagcatt
     1381 tcatagaaca gaagatcaga aagttaagtg tggttttgga cttcaggggg tttgttgtag
     1441 actctgttca aatggaccat gtagagtaac tcctaaatca ccaagaggta tatgtggagc
     1501 agatgcagac actatagtag caagaaactt tttaagagca gtagcatcag gagcagcttg
     1561 ttatttacat gtggtagaaa atacagctaa gaatttaaaa aatgttggaa taactaaagg
     1621 tgttgttaaa ggagaaaaaa cattaaatca attagcagag atgtttggaa tagaaagtga
     1681 tgaaaagtat gataaatgta taaaggttgc agacaaagtt atcaacgatt tgtataaatc
     1741 aagagatgat aaaatggaat tagtagaaaa aatagcttat gcacctagag tgaaaaagtg
     1801 gaaagagtta ggtataatgc ctggtggtgc aaagtcagaa gtttttgatg caatagtaaa
     1861 atcatcaaca aatttaaata gtgatccagt agacatgcta gttaattgtt tgaacttagg
     1921 tatatcaaca ggattatatg gtcttacact aactaatctt ttaaatgatg ttatgttggg
     1981 agaaccagtt ataagaatgg ctcctgtagg atttaatgta atagacccag actacataaa
     2041 tataatgata acaggccatc aacattcaac atttgcaaat ttccaagata gattaaaaga
     2101 tgaagatgtt ataaaattag ctcaatctgt tggtgcaaaa ggctttaagc tagtaggatg
     2161 tacatgtgtt ggtcaagatt tacaattaag aggagaacat tatcaagaag tatttgctgg
     2221 acatgcaggt aacaatttta caagtgaggc tgttttatca actggagcaa tagatatagt
     2281 tctttcagaa tttaactgta ctataccagg gcttgaacca atagcagata aatataaagt
     2341 aaaaatggtt tgtttagatg atgttgctaa aaaagcgaat gcagaataca taggtttaga
     2401 cagaagtaaa ttagatgaac ttagtaacac tttaatagaa aaagcattgg agtcttataa
     2461 ggagagaaga ggaagtattg agatagatat accaaaagac catggatttg aacaatcttt
     2521 aactggtgta agtgaaaaga acctgaaagc gtttttggga gattcatgga aacctcttat
     2581 taatttaatt gcagaaggaa aaattaaagg tgttgcagct gttgtaggat gttcaaatat
     2641 gacagcagga ggacatgatg taaatactgt tgaacttact aaagaattga ttaaaaaaga
     2701 tataatagta ctatctgcag gatgttctac tggaggatta gaaaatgttg gtcttatgtc
     2761 acctggagca gaagaattag caggagaaaa cttaaaagaa gtatgtaaga ctttaggaat
     2821 accaccagta ttaaactttg gaccatgtct agctatagga agactagaaa tagtagcaac
     2881 agaattagca gctgaacttg gaattgacct accacaatta ccattagtgc tttcagctcc
     2941 acaatggttg gaagaacaag cattagctga tggagcgttt ggtcttgccc ttggcttacc
     3001 tcttcattta gctttacctc catttataac aggaggaaaa ttggtgacag aagtattaac
     3061 agaaaaatta aaagacttaa caggaggaca tgttatagtg aatccagacc ctaaatcttc
     3121 agctaatcaa ttagaagaaa taataataga tagaagaaaa aatttagggt taaaggatgt
     3181 ggaatgcaat gcatagaatt tttataaata aagatttatg tacaggatgt aagagctgtg
     3241 tactagcatg tatgctaaaa cacaataaag attatgacat gtacactctt gatttagaaa
     3301 atatagataa tgatagtaga gggcatatag aattagatag taaacataat aatccagttc
     3361 caattctttg tagacactgt gatgaacctg aatgtgtatt agcatgtatg agtggtgcaa
     3421 tgcataaaga tagtgaaagt ggaatcgttt catatgatga agagaaatgt ggttcatgtt
     3481 atatgtgtgt aatgtcttgt ccatatggat tattaaagcc agatgataga agcaagcaaa
     3541 acatacttaa atgtgattta tgtaaagatg aggagtatcc tagatgtgta gctaattgcc
     3601 caagtggagc aattgaatta cagaaggagg aaaatgatga attatgtagt gttaggggct
     3661 agtgcagctg gtataaatgc agtaaaaaca ttaagagaat tagataaaga ttcaaatata
     3721 gtagtaattt ctaaagatga aaacgtatat tcaagatgta tgttgcatca cgttatatct
     3781 gaacacagaa ctttaaaaca aataaatttt gttgatgaag attttatgga gcaaaataat
     3841 gtaaaatgga ttgcaggtaa gactgttaaa ggtatagaca taaataaaaa ggtagttcaa
     3901 acagaagaca ttactgtaaa ttatgataaa cttttaatag ctacaggtgc atcttctgct
     3961 attcctccta taaaaaattt aagagatgga aattttgtat attctgtgag aaacatagat
     4021 gatatttata aaataaaaga aaaagcacaa aattctaaaa atgtagttat aataggtgct
     4081 ggtttagttg gtatagatgc attagtggga ttatttaaat atgagcagtt aaatatttct
     4141 gtagctttta tggaaaagta cattttagat agacaattgg atgaatatac agcatcagtg
     4201 tatgaaaata agttcaaaga aaaaggtgta aaattttacc caagtgcatc aatacaagaa
     4261 attgtattgg ataacagtaa aaatgttaca ggtgttgcat tttcaaatgg tgaagtttta
     4321 gatgcagaca tggtaattgt agctacaggg gttaaaccaa atgctgactt tttagatggg
     4381 acaggaatag aatatgatag aggtataata atagatgata tgtgccaaac aacacaaaaa
     4441 gacatatatg cagcaggaga tgttgtaggt aaaaatgcta tttggcctct ggcagtaaag
     4501 caaggaattg tagctgccta taatatggtt ggaaaagata agaagataga agatgaattt
     4561 gcttttaaaa atagtatgaa tttcatggat ataccaacta tttctatagg aatgaataca
     4621 cctgtagatg atagctataa agtattaacc agacatggaa ttaatgatta taagaagttt
     4681 gtttttaaag ataatgtaat ctacggagca gttatacaag gagatatttc atatgtaggt
     4741 gtattaactt atttaataaa aaataaagtt gaaatatatg atttagaaaa taggatattt
     4801 gatgtatgtt atgcagactt ttttaatata aaagaaaatg gagaattttg ttatagtgta
     4861 tagtatatac gtggagtaag gtttataaat acagtaaatc aataaataga gttattctaa
     4921 aataagaaaa atgacaaatc ttatttagaa atagctctat tttttgtaat taaatttaaa
     4981 ttatattcaa tttgtaaaat attattttga acaattatac atatactctc tagttagagg
     5041 taaatcattt tttatacctt tagtgaaaag tatttgatgt atatctataa taccagtgtt
     5101 gaaagcagca gcacaagaac aaaggtataa atcccacatt cttataaact tatcatcaaa
     5161 catttcttta atctttggaa gattctcaga gaaacttttt tgccagtata gtaaggtttt
     5221 tacataatgt aatcttaaac tttctacgtc tatagtatga tatttgtaat cactacaaat
     5281 atgtatcatt tctcttaggc taggaataac tcctcctggg aaaatatatt tttttatcca
     5341 agggtctcca taagattcct gcaatccact tatatagtga agtaaaaaca atccaccatc
     5401 ttttaaaaca gcatttacgt ttttaatgaa aagttcatag ttttctctac caacatgttc
     5461 aagcattcca acgcttacaa ctctatcaaa cataaggtct gattttttta attctctata
     5521 gtccataagt cttacctcaa gatagtcttg aaggttctct tctttaattc ttgcgttaaa
     5581 ttccttaaat tgctcttcgc taagtgtaat tccaacgccg tgaattttat atttttttgc
     5641 agcttctatt agtaaaaatc cccaaccaca accaatatcg agaaggctca taccttcctc
     5701 taaatttagt ttagttaata tgtggtgaac tttattgact tgagcatcat aaagagtatc
     5761 attatcattt ctaaagtatg cacaagaata actaagtgtc ttatctaacc ataagctata
     5821 aaaatcgttg ccaatgtcat agtgggcatg tatttctttc ttctgatgtc tcattgaatt
     5881 tgatgagtgc aatagatgtt ttacagctga attatcaata ttgaatttgt ctatttgttt
     5941 taaaagtaga tttaaagtta agaataaatc tccctcaact tcaatgtctc tatttatgta
     6001 ggcttctcca agtgctaaag atgtactttt taatagttca gacttgctaa tatcattatt
     6061 tactataact ttgaactgag gggagccctc tccaattaga aattcttcct catctttaag
     6121 cctaactaga aatggtgttt caccaaattt ctctaaatag tcttttaaaa aattttttgt
     6181 aatattcata ttaccagctc cttctaatgt aaatccagta agtttccctg cttaaaatat
     6241 attatagtcc tttttttaaa aaagtaaaat tttttcaata aattttttat ttaaaggaaa
     6301 tttatttttt ataaagaaat ttcatgttgt agtatatttt ttgcttataa attttggtac
     6361 tggatataat attaaactgt taatgatata ataaatttaa tctataaagt aaataaaatt
     6421 ttgtaataaa tagttaaagc tattttataa ggagatatat gatggaagta tatgctttgg
     6481 ttgggtcaag tggcacaggg aagagcttta aggctttaga gttcgcatat gaaaatgata
     6541 tagaatatat aatcgatgat gggattttaa tatacaaaaa caaggtttta gctgggatat
     6601 cggcaaaaca agcaaatact actatagaag cagttaaaag ggctattttt tataatctgg
     6661 agcatagaca agaagttaga gaagcaatta aaaaagaaaa tataagaaaa attcttatta
     6721 ttgggacatc aaaaaaaatg gttaatcaaa ttgtagagag attgtcaatt ggaagggtct
     6781 gtaagtttat aaatatacaa gatataagta ctaaatatga aatagaaata gcaaaacaag
     6841 ctagaaaaga aggaaatcat gtaattcctg ttccagccgt tgagataaaa agtatggcaa
     6901 gtggtctcag tataaattct ttgaagagac tttttagaaa aggaaataat aggaatatgg
     6961 tagttttaga aaaaacaatt ataagaccaa cttttagtta tataggaaag ttttatatta
     7021 gtgctgatgt aattaaacag attattgaat atgaagtata caactttgga agcattgata
     7081 gaataaataa aatcaatata gagaattgca ataattttat gaatattttt gtaagtataa
     7141 atatcaatga tttagaaact ataaaaaaaa ttgaagacat acaacagaca ataaaaaagt
     7201 ctattgagaa aatgacttta gttaatgtac aaaaaataga tattaacatt cataaaatta
     7261 aaaaaataaa cttcctccaa tgttaaaaaa taaactgaga aaatgatata ctaatttgca
     7321 ataaagtgtg ctataattaa actgtaaatt taggaaatgg ttttttctaa atggagtata
     7381 cataattata acgtatttat aagggggctt tcaaatgtca ggaaaagatg taaatgtctt
     7441 cgagatggcg caatctcaag taaaaaatgc atgtgataaa ttaggtatgg aaccagcagt
     7501 ttatgaatta ttaaaagaac ctatgagagt tatagaggtt tcaattccag taaaaatgga
     7561 tgatggttct ataaaaactt ttaaaggatt tagatcacaa cataatgatg cagtagggcc
     7621 aacaaaaggt ggaataagat tccatcaaaa tgtttcaaga gacgaagtaa aagctttatc
     7681 tatatggatg actttcaagt gttctgtaac aggtatacca tatggtggag gtaaaggtgg
     7741 aataatagta gatccatcaa ctttatctca aggtgaatta gaaagattaa gtagaggata
     7801 catagatgga atatataaat taataggtga aaaagttgac gttcctgcac cagacgtaaa
     7861 tactaatggt caaataatgt cttggatggt tgatgagtac aataaattaa ctggacaaag
     7921 ttctataggt gttataactg gtaagccagt tgaatttggt ggttctttag gaagaacagc
     7981 tgctactgga tttggtgttg ctgttactgc aagagaagct gctgctaaat taggaataga
     8041 catgaaaaaa gctaaaatag ctgttcaagg tataggaaac gttggttctt atacagttct
     8101 taactgtgaa aaacttggtg gtactgttgt agctatggct gaatggtgta aatcagaagg
     8161 ttcttatgct atatacaatg aaaatggttt agatggtcaa gctatgttag attatatgaa
     8221 agaacatggt aacttattaa acttcccagg agctaagaga atatctttag aagagttctg
     8281 ggcttcagat gttgatatag taataccagc tgcattagaa aactctataa ctaaagaagt
     8341 tgctgaatct ataaaagcta aattagtttg tgaggctgct aatggaccaa ctactccaga
     8401 ggctgacgaa gtatttgctg aaagaggaat agttcttact ccagatatat taactaatgc
     8461 tggtggagtt acagtttctt acttcgagtg ggtacaaaac ttatatggat actactggtc
     8521 agaagaagaa gtagagcaaa aagaagaaat agctatggtt aaagcatttg aatctatttg
     8581 gaaaatcaaa gaagagtaca atgttacaat gagagaagct gcttacatgc attcaattaa
     8641 gaaagttgct gaagctatga aattaagagg atggtactaa gatttactct taataaataa
     8701 taggatataa ttatttaaga tataaagggg ctgtacatag tacagccctt aaattatgtg
     8761 gcttacaatg aagtaagcta tttttgtttt aacttataaa taacttgata aattatgtaa
     8821 tttagaatgc ataaataaga tgggtgaaaa ttaatggttg atttaatttt gaatgtattt
     8881 tttaccacca catgattaca aattagagga gttagattgg atttttggac atgaacttat
     8941 gtattttaaa agcaaagacc ttcatttaaa gtatctagta ttatttttaa aaatagtata
     9001 ttggtttaat ccatttatat atataatgga aaaatatatg gatttggatt gtgaacttta
     9061 ttgtgatgag agagttttaa aaaattgtac tgtacaacaa aagcaagaat atgctctaac
     9121 tattgtaaat tcaatgaaaa gaggtataaa tttttcaaat aaatttgtaa ctggattaaa
     9181 taaacaatct gatattagaa aaagagtaat gaacatgttc agtgaaaaac ataaaaatgg
     9241 tatcttaatg gctttgatta taagcttgtt ttcttctatt acatttttta aattcgattc
     9301 aactagtctt attgaagaga atgatttctt agagaggtca agatatgcgt ttgaaggaag
     9361 gcaagaagac atgtttgtaa caatagattc tacatatgca gatgcaccta taaaacttag
     9421 aaaagagcat gaagaaaact gtagagtcaa tggaatagta ccaaaagatt cagattcaat
     9481 agaaattatt cctagatttt ataaagattg tatggattta aaaaaataag ttagacttaa
     9541 tattgatgtt gatattatga attttttata tttaagcaat tgaaaatttt attatagatt
     9601 caattttata tgtgtttaag tatgaaattt atagatagtt gaaataatta aatatgattg
     9661 atgcatagga atcattacta agctataatt aattataggg gagtttaaat aaattatgag
     9721 ttacacaatt ataaaatgat aaaaaatagt tatctgttac aaaaaatgta tctttttaat
     9781 taataaagat aatatgaaaa cttaaaaggc aaataaactt aaacataaaa tactcttaaa
     9841 taagatagga gaaaaatgta tgtctaactt tattctaaat gggattttta gaacagttat
     9901 agtaagtagt ctaagcattc ttctgatatt gattttcaag aaaaatgtat ttaaaagatt
     9961 tagcaaaaaa tttaattatt atatatggat gatagttgtt attaaattat ttttaccatt
    10021 tacatattat acttttacaa taaatatctt gagaagtaaa aaacacatta atattaataa
    10081 cataaattta gagggattta ataatgtttc aactataagt a
//
LOCUS       DAMJWP010000123        10050 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.128, whole genome shotgun sequence.
ACCESSION   DAMJWP010000123 DAMJWP010000000
VERSION     DAMJWP010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10050
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.128"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..1323
                     /locus_tag="PC700_002437"
     CDS             <1..1323
                     /locus_tag="PC700_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HDF2581739.1"
                     /translation="SNAKLIEQLKKLAYYDSLSGIKNKEKFRKDSMYILKNYYQDNFY
                     LVQLDVNKFKYINEMFGYAEGNKILIHISQVLNNNTNKYEICARMDNDHFILLIACNT
                     EDELLNRLSKINKEICNLNTTNSSKYKIVMSSGIYKITKKDDIKKIDLLIDRANIAAK
                     SKKEKYEHSYSFFNEDTRNRLYKEKRLEDNMNKALEKGEFIVYYQPKYSLDDVNEIEG
                     AEALIRWNSPEFGFISPIDFVPLFEKNGFIVNIDMFVFEEVCKTLNKWINKGYTPVPI
                     SVNMSRVHLYRDNFIENITDLISKYNISPEFIELELTESVVFDNLNILIDIMKKIKEI
                     GFLISMDDFGSGYSSLNLLKDLSFDILKLDRGFLIETTDTKRGKIIISKIVEMAKAID
                     IKVICEGVETYEQVEFLKEIGCDKVQGYLFAKPMVLDEFEKHLNFKFD"
     gene            1470..2216
                     /locus_tag="PC700_002438"
     CDS             1470..2216
                     /locus_tag="PC700_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2581740.1"
                     /translation="MNILSIRNISKTYCGNIPFKALDKVSLNIEKGEFVSVMGPSGSG
                     KSTLLNIISTVDRQSEGEVVLDGYDVSKLKGEKLAEFRRKQLGFVFQDFNLIDTLTVG
                     ENIMLPLTLEGASIKDMNIQTKSISKFLGIDKILDRKTYEISGGQAQRCAIARAIINK
                     PTILLADEPTGNLDSKSTDDVLKLFTRINKEQNVTTLMVTHEVYSASYSDRVIFIKDG
                     CIYTEIKKSESSNSFYSDILAVLSQIGGVR"
     gene            2216..4096
                     /locus_tag="PC700_002439"
     CDS             2216..4096
                     /locus_tag="PC700_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2581741.1"
                     /translation="MDFRKFAYNNIVRNFRAYLAYFLSSVFSIMIFFVFAVSMFHPII
                     TKSGMQSGSTAFMALMTSELTILGFSLLFILYSLGNFLKYRLKDFGVLMIIGMSNRQL
                     KKLILIENLIIGFLAVMLGILLGLSISKLFLLYLSKLFYMNLTEIYFPVKAILLTIVS
                     FMILFLITGPMSLKLLRKKSILELLVGTKKPKKEIKSSRLFGIIGVICLILGYAMILF
                     GNKLKINGGMYTTALLITLGIFLFFSQFTILILKYLKNKEKFYKNKVNMLLISNLIYK
                     LKDNTRLLFLITILLSATLVAFTTTSTLVSSQGEASKNNFPMVYSYFSEHNNEKEYEE
                     LQQIREAIKGYDYKELSFPVLKYNREVLLSVKNYNKLSKELGLDKISSKLEKYEGIII
                     PSSNSQEHINSLKQLKNYSIKNLNIEVKKSKNKIIFPTGLFSKVVVISDDLYDEVKGE
                     FSKINFHGFDYKNWQSSAKITENLKEKHFNLDREYNRSYFLTLPDMYLVELQQSKILQ
                     FMGVFIGVILFIAVFSFLYFRLYTDEIEDNVKYKKLSKIGLSFKNMNKIVSIEIGTLF
                     FIPYFIAIINSIISLLFLNQVLNNSFRLENLGILLLISSIYSLYFYLLKKIYTRKVWF
                     FD"
     gene            4215..5606
                     /locus_tag="PC700_002440"
     CDS             4215..5606
                     /locus_tag="PC700_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2581742.1"
                     /translation="MENQQLLGTERISKLLLKYSIPAIIGMLVNSLYNVVDRIFIGNI
                     PGVGPLAITGLGVTMPIMTIILAFGMLIGIGTTTTISIKLGQGKVEEARKLIGNAMTL
                     SVITGIIIMILGILFANKILTLFGASENTLIYAKSYINIILLGTVVNLLSFSLNHSIR
                     ADGSPKISAGIMIVGCLTNIVLDWILIFGFNLGIQGAAIATVTSQALTAILTIGYYIS
                     GKSNLRFSKSNLKLDKKLIKAVFAIGMSPFAMQLAASLVQVISNIALKTHGGDLAIGA
                     MATISSIAMVFLMPIFGINQGAQPIIGFNYGAEKYDRVKKAYLGSLVVATIILCMGMV
                     VVMLFPEAIIGIFNKDPELMNISVNGLRIYLLMLPIVGLSVTGTNFIQSIGKAKMAML
                     LSLLRQVILLIPAVLILPTFLGLQGVWTAQPVSDFIATVITGIVVFRELKRYTPKTEK
                     LNENERLNEITTE"
     gene            5713..6753
                     /locus_tag="PC700_002441"
     CDS             5713..6753
                     /locus_tag="PC700_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDF2581743.1"
                     /translation="MEFTKKSKLKNMYVNMNVARMLSKINEYKGRQLLYKKQPKEILE
                     NLEKKSLVDCSESTYIGNQRDSSNFNLEKLISNEVTPRSREELSIVEYRDVVKTINSA
                     YESIPISSQTILELHGYLYKFSSTRGGSYKSDNDFIEHNLKPSEFISIDSSVNKVEKA
                     VEEICEAYNTLIEEDEIDILILISAFVLDFILIHPFKEGNIKMARVLILLLLNKNGYE
                     VGRYISLGKIFDDSSYEYYSNLNYLKASIGSEKADMNAWIEYFLETILTAYEKLDDSL
                     NISDKKRQTKTSRIEKIINSTLGYFTKEDIRDLCPDIPEPTINRVFNNLRKQDKIEVV
                     ARGRSAKWKKKY"
     gene            7242..7895
                     /locus_tag="PC700_002442"
     CDS             7242..7895
                     /locus_tag="PC700_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDF2581744.1"
                     /translation="MKKKLLYINVNSKPEDLSSSKTVARKFINKFMEKNKDFEVEEID
                     LYKEHIPRLEYQYFEKRNSIVSEENAKNLDDKDRKELTKIRNLCDQFVSASVYVIAAP
                     MWSLSFPAPLKEYIDCIIQDGKTISFEGNDKPQGILNDIDRSMVYIQSSGGHIPWVLK
                     PVMNKGLNYVESIMKFIGIKKFDELLVDGTGTSEEERQAAIEKASEKIHGIIDGMKF"
     gene            7971..8180
                     /locus_tag="PC700_002443"
     CDS             7971..8180
                     /locus_tag="PC700_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581745.1"
                     /translation="MREEKSNEKYDCYWCNQENNFCVEIKDNIVMIDDGTGTLKQAVF
                     IGYKQIQINLNCSHCQNLNRIKLNL"
     gene            complement(8320..8886)
                     /locus_tag="PC700_002444"
     CDS             complement(8320..8886)
                     /locus_tag="PC700_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HDF2581746.1"
                     /translation="MMNNELNTIILETLNNADITSNDIPSIDLYMDQIISLIDNKLSP
                     NKRFESDKILTKTMINNYSKEGLIKPVKGKKYTKEQILQMIIIYSMKNTLTIQEIKRI
                     LHGVYEKDNFSEKDLVSCYEKFMLIKENQRKNIPDFIESNFENISINPENKDDLLIAL
                     LSLTSMADQLKNISEKLVDRYFPDITKK"
     gene            9047..9700
                     /locus_tag="PC700_002445"
     CDS             9047..9700
                     /locus_tag="PC700_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDF2581747.1"
                     /translation="MYQFFLKGRDPISSLTHFIGACLSLLATIILVFQSVTLQETSLL
                     MIVSVSVFGLSLIALYSASSYYHFLKGTPEQELFFRKVDHAMIYVLIAGSYTPICLNF
                     MEKKEGIIFVTAIWIVAFIGIIIKIFWMDAPRWLSTSIYLLMGWAIVFDINAFNSIPK
                     DCLRLLIMEGVSYSIGAIIYIIKKPNISPEFGFHEIFHIFIMIGSLFHFLAVLLYVL"
     gene            complement(9858..10034)
                     /locus_tag="PC700_002446"
     CDS             complement(9858..10034)
                     /locus_tag="PC700_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581748.1"
                     /translation="MYKYDDYVNLTNDLDLPVEIRNKCVRIVSVSENLEDIIIVHKFK
                     LYVVNEKYIKGIME"
BASE COUNT         3931 a          992 c         1606 g         3521 t
ORIGIN      
        1 agtaatgcta aattaataga acaacttaaa aaattggctt attatgattc tttaagtgga
       61 ataaaaaata aagaaaagtt tagaaaagat tctatgtata ttttaaagaa ttattatcaa
      121 gataattttt acttagtaca attggatgtc aataaattta aatatataaa tgagatgttt
      181 ggatatgctg aagggaataa gatattaatt catatatcac aggttttaaa taataataca
      241 aataaatatg aaatttgtgc taggatggat aatgaccatt ttatattgtt gattgcttgt
      301 aatactgaag atgaactttt aaatagatta tctaaaataa ataaagaaat ttgtaactta
      361 aatactacta actcatctaa gtataaaatt gttatgtcaa gcggaatata taagattact
      421 aaaaaggatg atattaaaaa gattgacctg ttaattgata gagcaaatat tgcagcaaaa
      481 agtaaaaagg aaaaatatga acattcatat tcgtttttta atgaagatac aagaaatcga
      541 ttgtacaagg aaaaacgatt ggaagataat atgaataaag ctttagaaaa aggtgaattt
      601 atagtatatt atcaacctaa gtatagttta gatgatgtaa atgaaattga aggagcagaa
      661 gccttgataa gatggaatag tccagaattt gggtttattt cacctataga ttttgtacct
      721 ttatttgaaa agaatggttt tattgtaaat attgatatgt tcgtgtttga agaagtatgc
      781 aaaacattaa acaagtggat aaataaaggg tatactcctg taccaatctc tgtaaatatg
      841 tctagagttc atttatatag ggataatttt atagaaaata taactgattt gataagtaaa
      901 tacaatatat ctcctgagtt tattgaactt gaactaacag aaagtgttgt gtttgataat
      961 ttaaatatat tgattgatat aatgaaaaag attaaagaga taggattcct tatatcaatg
     1021 gatgattttg gttcgggata ttcatcttta aatctgttaa aagacctttc ttttgatatt
     1081 ttaaaattag atagaggatt tttaatcgaa acaacagata caaaacgtgg aaaaattatt
     1141 atttctaaga ttgtggaaat ggcaaaagcg attgatatta aagtaatctg tgagggcgta
     1201 gaaacttatg aacaagtaga gttcttgaaa gagataggtt gtgataaggt tcaaggatac
     1261 ttatttgcta aacctatggt attagatgaa tttgaaaaac acttaaactt taaatttgat
     1321 taatcattaa taattattag actataatat accctaacaa cttacttaca aatatgtaag
     1381 ttaattgtta gggtattttt atggttaaag tagtatattc acgatattat ggtattgtag
     1441 taaaaataaa aaattatgga gaatataaaa tgaacatatt gagtattaga aatatatcaa
     1501 aaacatattg cggaaatatt ccatttaaag cattagataa ggttagtcta aatatagaaa
     1561 agggagaatt tgtgtctgtt atgggaccat ctggaagtgg taaatctact cttttaaata
     1621 taatatctac agttgataga cagagtgaag gtgaggttgt attagatgga tatgatgtaa
     1681 gtaaattgaa aggagaaaaa cttgctgaat ttagaaggaa acaacttggt tttgtatttc
     1741 aggattttaa tttgattgac actttaactg ttggcgaaaa tattatgttg ccacttacac
     1801 tagagggggc aagtatcaaa gatatgaata tacaaactaa aagtatatct aaatttttag
     1861 gaatagataa aattttagat agaaaaacct atgaaatttc aggaggtcaa gctcaaaggt
     1921 gtgctattgc tagagctatt ataaataaac ctaccatact tcttgcagat gagccaactg
     1981 gaaatcttga ttcaaaatct actgatgatg ttttgaaatt atttactagg ataaacaaag
     2041 aacaaaatgt aacaacactt atggtaactc atgaagtata tagtgcaagt tattcagata
     2101 gagtaatttt tataaaagat ggttgtatat atacagaaat aaaaaaatct gaaagcagta
     2161 attctttcta ttctgatata ctagctgttt tgtctcaaat aggaggtgta agatagtgga
     2221 ttttagaaag tttgcttata ataatatagt tagaaatttc agggcatatt tagcatattt
     2281 tttaagttct gttttttcta taatgatatt ttttgttttt gcagtgtcta tgttccatcc
     2341 gattataaca aaatctggaa tgcagagtgg ttctactgca tttatggctc ttatgacttc
     2401 agaattaaca atattaggtt ttagtctact ttttatattg tactctttag gaaatttctt
     2461 gaaatataga ttgaaagatt ttggagtatt gatgataatt ggaatgagca acagacaact
     2521 gaaaaaattg atattaattg aaaatttgat aataggattt ttagcagtga tgttaggaat
     2581 attacttggt ttatcaattt caaaattgtt tttattgtat ttaagcaaac tgttttatat
     2641 gaatttaact gaaatttatt ttccagtaaa agcaatacta ttgactattg tatcatttat
     2701 gattttattt ttgataacag gaccaatgag cttaaaatta ttgagaaaaa agagtatttt
     2761 agagctttta gtaggtacaa aaaagcctaa aaaagagata aaatcttcaa ggctatttgg
     2821 aataattggt gtaatatgtc ttatattagg atatgctatg atactatttg gcaataagtt
     2881 aaaaataaat ggaggtatgt atacaacagc actattaatt actttgggga tatttttatt
     2941 tttttctcaa tttactattt tgatattaaa gtatttaaaa aataaagaaa aattttataa
     3001 gaacaaagta aatatgttac ttataagtaa tttgatatat aaattaaagg ataatacacg
     3061 attgttattt ttaataacaa tacttttatc ggctacttta gtagcattta caacaacttc
     3121 aacacttgta agttctcaag gagaagcttc taaaaataat ttcccaatgg tttactcata
     3181 tttttcagag cataataatg aaaaagaata tgaagaacta caacaaatta gagaagcaat
     3241 caaaggatat gattataagg agttaagttt tcctgtactt aaatataata gggaagtctt
     3301 actaagtgta aagaattata acaaattatc aaaggaattg ggattagata aaattagttc
     3361 aaagttagaa aaatatgaag ggattataat tcctagtagc aattcacaag aacatataaa
     3421 ttctttaaaa caattaaaaa attatagtat aaaaaattta aatatagaag taaaaaaatc
     3481 aaaaaataaa ataatttttc ctacaggact gtttagtaaa gttgtagtta taagtgatga
     3541 cttatacgat gaagtaaaag gtgaattttc aaaaattaat tttcatggat ttgattataa
     3601 aaactggcaa agcagtgcaa agataactga aaatttaaaa gaaaaacatt ttaatttgga
     3661 tagagaatat aacaggtcat attttttaac attaccagat atgtatttag tagagttaca
     3721 acaaagtaaa attcttcaat ttatgggtgt ttttatagga gtgatattat ttatagcagt
     3781 ttttagtttt ttatacttca gattatatac agacgagata gaagataatg taaagtacaa
     3841 aaaactatca aaaataggtc tatcttttaa aaatatgaat aagattgtta gtatagagat
     3901 aggtacattg ttttttatac cgtattttat agcaattata aatagcataa tttctttact
     3961 gtttttaaat caagtattaa ataatagttt taggttagaa aatttgggta tattactact
     4021 gatatcatct atatattcac tttattttta cttattaaaa aaaatttata ctagaaaagt
     4081 ttggtttttt gattaaagtg gtatataata gatatataaa ttaatattta aaaagtagtt
     4141 aatattaggg gagggcattt gcacctccct tgtaatatgt ggaaaattga atattagaag
     4201 aaggaggaga cttgatggaa aatcaacaat tattaggaac agaacgaatt agtaaattac
     4261 tactaaaata ttctatacca gctataatag gtatgttggt aaatagttta tataatgtag
     4321 tagatagaat ttttattgga aatataccag gggttggacc acttgcaata acaggtcttg
     4381 gtgtaacaat gccaataatg acaataatac ttgcatttgg gatgttgata ggtataggaa
     4441 ctactactac catatcaata aaacttgggc aaggtaaagt agaagaggca agaaaactta
     4501 taggaaatgc aatgacgtta tctgtaataa caggaattat cataatgata cttggaatat
     4561 tatttgcaaa taaaatactt acattgtttg gagcaagtga aaatacttta atatatgcaa
     4621 aatcatatat aaatattata ttattaggta cagtagtaaa tcttctttcc ttttctttaa
     4681 accactcaat aagagctgat ggtagtccta aaatttcagc aggaataatg atagtaggat
     4741 gtttaactaa tatagtttta gactggatat taatatttgg attcaatttg ggaatacaag
     4801 gagctgcaat tgctacagta acttctcagg ctcttacagc tattttaact atagggtatt
     4861 atataagtgg aaaatcaaac ttacgattca gtaaatctaa tctaaaatta gataaaaagc
     4921 taataaaagc agtgtttgca ataggtatgt cgccatttgc gatgcaactt gcagcaagtt
     4981 tagttcaagt aatatcaaat attgcactta aaactcatgg tggagattta gctatagggg
     5041 ctatggctac tatttcttcc attgcaatgg tatttttgat gcctatcttt ggaataaatc
     5101 aaggagcgca acctattata ggtttcaatt atggagctga aaaatatgat agggttaaaa
     5161 aagcatattt aggttcatta gtagtagcta ctataatttt atgtatgggt atggtagtag
     5221 ttatgctttt cccagaagct attataggta tatttaataa agaccctgaa cttatgaata
     5281 tatctgttaa tggattaaga atatacttac ttatgttgcc aattgtaggc ttatcagtta
     5341 caggaacaaa ctttatacaa tctataggaa aagcaaaaat ggctatgtta ttgagccttt
     5401 taagacaagt aattttattg ataccagcag tattaattct tcctacattc ttaggtttac
     5461 agggtgtatg gacagctcaa ccagtgtctg attttatagc tacagtaatt acaggtatag
     5521 ttgtatttag agaattaaaa agatatactc ctaaaactga aaaattaaat gaaaatgaaa
     5581 gactaaatga gataactact gagtaaaaat ataaattcag ataattgagc aagaaattgc
     5641 aaaaaatacg attatatgat aaaataaaac cgctgtcaag gataagagaa attctaatta
     5701 tggagtgatt ttatggaatt tacaaagaaa agcaaattaa aaaatatgta tgtaaacatg
     5761 aatgtagcta gaatgttgtc taagataaat gagtataagg gtaggcaact gctttacaaa
     5821 aaacaaccaa aagaaatttt agaaaaccta gaaaaaaaat ctttagtaga ttgttctgaa
     5881 tctacataca ttggaaatca aagagatagt agtaatttta atctagaaaa gttgataagt
     5941 aatgaagtta cacctagaag tagagaggaa ttgagtattg ttgaatacag agatgtggta
     6001 aaaactatta acagtgctta tgagagtatt cccatcagtt cacaaacgat tcttgagctt
     6061 catgggtact tatataaatt ttcatctact agaggaggaa gttataagtc agataatgat
     6121 tttattgaac acaatttaaa acctagcgaa tttataagta ttgattcaag tgtcaataaa
     6181 gttgaaaaag ctgtagaaga aatatgtgaa gcttataata cattgataga ggaagatgaa
     6241 attgatatat taatattaat ttctgcattt gtgttagatt tcatattgat acatccattc
     6301 aaagaaggta atataaaaat ggcgagagta cttatcctat tgttactaaa taaaaatggg
     6361 tatgaagttg gaagatatat aagtttagga aagatatttg atgatagttc atatgaatat
     6421 tatagtaatt taaattattt gaaagcctca ataggtagtg aaaaagcaga tatgaatgca
     6481 tggattgagt attttttaga gacgatatta actgcatatg agaagctgga tgatagttta
     6541 aatatatctg ataagaaaag acaaacaaaa actagtagaa tagaaaaaat aataaattct
     6601 acccttggat attttacaaa agaagatata agagatttat gtcctgacat tccagaacct
     6661 actataaata gagtttttaa taatctaaga aaacaagata agatagaagt tgttgcaaga
     6721 ggaagaagtg caaaatggaa gaaaaaatat taaaaaatgt ttaaataact aaaatggtat
     6781 agtgttttac actataccat ttttaatgtc attagcatga gcaataattt aacaacttaa
     6841 gcaatatcaa ttttatttat tgattatttt tgaactgaaa aatggggtta tatggtatac
     6901 tacaataata attaattttg gatgagaata tataacgtta cagaatatct taggagtata
     6961 tgtataaaaa atataataaa agtgtgaggt ttagtaatta acaaattatg tggcttcaca
     7021 cttttttata aaataaaaat acttagcaga agaatatttt agttgatatg tttataagat
     7081 agtacttgtt tgggtaaaag tataaatagt aaaaaagtag aagtaatgtg tataaaaaca
     7141 taagttacat tctgtaataa tagttatttt ataattcaac aaaagaatag aatgggtata
     7201 tttgtgaaat aataatataa aataatctta ggagtgatct tatgaagaaa aaattattat
     7261 atataaatgt aaattcaaaa cctgaagact tatcttcaag taaaactgta gcaagaaaat
     7321 ttataaataa atttatggaa aaaaacaaag attttgaggt tgaagagata gacctttata
     7381 aagaacatat accgagactt gagtatcagt actttgaaaa aagaaatagc atagttagtg
     7441 aagaaaatgc taaaaattta gacgataagg atagaaaaga gcttacaaag attagaaatc
     7501 tttgtgacca atttgtgagt gcaagtgttt atgtaatagc agcacctatg tggagtttat
     7561 cttttccagc accacttaaa gagtatatag attgtataat tcaagatgga aaaactatat
     7621 cttttgaagg caatgataaa ccacaaggaa ttttaaatga tattgataga agtatggtat
     7681 atatacaatc ttctggtgga cacataccat gggtcttgaa gccagttatg aataaagggt
     7741 taaattatgt ggaaagtatt atgaaattta taggaattaa aaaatttgat gaattgttag
     7801 tagatggaac tggaactagt gaagaagaaa gacaagctgc aatagaaaaa gcatctgaaa
     7861 aaatacatgg tataatagat ggtatgaaat tttaattata atattttatt aaaaatataa
     7921 aggaaaaacc tcttttaatg tagaaactta tattgaagga ggcgagaatc atgcgagaag
     7981 aaaaaagtaa tgaaaagtat gattgttatt ggtgtaatca agaaaataat ttctgtgtag
     8041 aaataaaaga taatatagta atgatagatg atggcactgg tacgttaaaa caagccgttt
     8101 tcatagggta taaacaaatc caaattaatt taaattgttc acattgtcaa aacttaaata
     8161 gaataaaatt aaatttgtag aattttaaaa tggaaattat tgaaggaatt aaaaatagaa
     8221 agtgttaata tttagactga aaattatatt ttatacaata aaagtttatt taaataacat
     8281 tgtattttaa tatttatttt cagtcttact ttattaaaat tattttttgg ttatgtctgg
     8341 aaaatatctg tctactaatt tttctgaaat attttttaat tggtcagcca ttgaagtcag
     8401 acttaataag gctatcaata aatcatcttt attttctgga tttatactaa tattttcaaa
     8461 attagactct ataaagtccg gtatattctt tctttgattt tcttttatta acatgaattt
     8521 ttcgtaacat gaaactaaat ctttttcact gaaattatcc ttttcataca caccatgtaa
     8581 gattctttta atttcttgta ttgtaagtgt atttttcata gaataaataa ttatcatctg
     8641 taaaatttgt tctttagtat acttttttcc cttaactggt ttgattaatc cctctttgct
     8701 gtaattattt atcatggttt tagtaagaat tttatcactt tcaaatcttt tattaggaga
     8761 aagtttattg tcaattagag aaataatttg atccatatat aagtcaatag agggaatatc
     8821 gtttgaagtt atatctgcat tatttagtgt ttctagtatt atagtattaa gttcattatt
     8881 catcatatgt tacctccata atatatataa tacactaaat tagaaagtaa aacaatatta
     8941 agacttgtaa aaaaaacata aagtggtata attaaatata taatatagta atgaaaacta
     9001 tatttatgaa tctgattttt aaaatacata attgtaggag gacgaaatgt atcaattttt
     9061 tttaaagggt agagacccaa taagcagttt aactcatttt ataggtgctt gtttatctct
     9121 tttagcaact ataattttag tttttcaatc tgttacatta caggaaactt ctctcttgat
     9181 gatagtatct gtatcggtat ttggactttc actaattgct ttatatagtg caagttcgta
     9241 ttatcatttt ttaaagggga caccagaaca agaattgttt tttagaaaag tagaccatgc
     9301 aatgatttat gtattgattg ctggctctta tacaccaata tgtctgaatt ttatggaaaa
     9361 aaaagaaggc ataatatttg ttacagcaat atggatagtt gcatttattg gcataataat
     9421 aaaaatattt tggatggatg cacctagatg gttatctacc agtatatatt tacttatggg
     9481 gtgggcaatt gtttttgata taaatgcatt caattcaatt cctaaggatt gccttagact
     9541 attaattatg gaaggtgtat cttactctat aggtgcaatt atttatataa ttaaaaagcc
     9601 taatataagt cctgagtttg gatttcatga aatatttcat atttttatta tgataggttc
     9661 tttatttcac tttttagcag ttttattgta tgttttatag ttttaaaaga tttgattaat
     9721 tgatactaaa attctacaat gaataaaatg gaactgcctt atatagatta tatcttcgac
     9781 aataagacag ttccttatta taaatattaa attctactta taataaaatt ttgataaaaa
     9841 tagtccattc aaaatgccta ttccataatt cctttaatgt acttttcatt tacgacatat
     9901 agtttgaatt tatgtactat aattatatcc tctaaattct ctgaaacact aacaattctg
     9961 acacatttgt ttctaatttc tacaggtaaa tctaaatcat ttgttaaatt cacatagtca
    10021 tcatatttat acataaaatc atccctttac
//
LOCUS       DAMJWP010000124         9953 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.129, whole genome shotgun sequence.
ACCESSION   DAMJWP010000124 DAMJWP010000000
VERSION     DAMJWP010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9953
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.129"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..1030
                     /locus_tag="PC700_002447"
     CDS             <1..1030
                     /locus_tag="PC700_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HDF2581749.1"
                     /translation="KKIKKLFLDDKGYYFNSNWEDIHIGIYDTKLGDSLKYITNEYIE
                     NMERVKYDYRELKNIENQTDGMCILAIRQNYKALEYVKNQTEELCLEAIKYNYKALEY
                     VKEQTEYLCLEAIKKDCNALKYVRNKTEGLIIKAISHSSNIDVVSILKALETQTRRIC
                     LEAIKKDGRCLAYVREQSEELCIEAIKQNYKALKYVKNQTEKMCIESVRQNGMALQYV
                     NKQTDKICIEAVKQDGRSLQFVNNKTEEICINAIRYLNKKYNIKDVLSYIDKYTEDIC
                     IEIVRQNGKMLMYIKNQTEKMCIEAVKENYKSLKYVKEQSERICKEALKQNHKAKEYV
                     KIAIDDCI"
     gene            1169..1621
                     /gene="tsaE"
                     /locus_tag="PC700_002448"
     CDS             1169..1621
                     /gene="tsaE"
                     /locus_tag="PC700_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDF2581750.1"
                     /translation="MAKIYLENENKTREIGYKLGKLLKEGSVICLVGDLGAGKTTMTQ
                     SLADSLGIEDYITSPTFTIINEYEGKIPLYHFDVYRIGSSDEMYDIGYDEYVNSNGIC
                     IIEWANLIEDILPKEYLNIELRYKDEGREMILTPKGEFYKEIVEELIK"
     gene            1621..2337
                     /gene="tsaB"
                     /locus_tag="PC700_002449"
     CDS             1621..2337
                     /gene="tsaB"
                     /locus_tag="PC700_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDF2581751.1"
                     /translation="MKILGMDTSSMAASVAVVEDDNLICEFTVNNKKTHSQKLMPMIE
                     NMLSMSDLSIKDMDLLAVCIGPGSFTGLRIGMATVKAMAHVNNIPIIAVNSLESLANN
                     INFCDRKICCILDAQKNQVYSAKYKFENGEMIELDGVDVVEFDALVDEIVSTNEEFII
                     VGEAVYKYKDKLENIKNIKIPSPANNVSNAGSLCSLALNKYNKNIDVHTCYTINPMYI
                     RKSQAEVQYDEKMKRLNNGR"
     gene            2327..2803
                     /gene="rimI"
                     /locus_tag="PC700_002450"
     CDS             2327..2803
                     /gene="rimI"
                     /locus_tag="PC700_002450"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDF2581752.1"
                     /translation="MEDNTKLIVDDIKIEEMTTEDIDEVFEVEKNCFEDYWSKESFRK
                     ELSNEVARYIVAKLNGKIVGYVGIWLILDEGHINNVAVHSDYRGKKIGDKLIKCIVDL
                     CKDNNIASMTLEVRASNKIAQNLYRKYGFKMGGIRKEYYSDNKEDAIIMWNQLKEV"
     gene            2809..3825
                     /gene="tsaD"
                     /locus_tag="PC700_002451"
     CDS             2809..3825
                     /gene="tsaD"
                     /locus_tag="PC700_002451"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDF2581753.1"
                     /translation="MSDIITLAIESSCDETAASVLKNGREVLSNIISTQIETHKKFGG
                     VVPEVASRKHVENIDIVVQEALDKANIGFNDIDHIAVTYGPGLVGALLVGLSYAKALA
                     YTLNIPLVGVNHIEGHLSANYIEHKDLKPPFITLIVSGGHTHLVEVKDYGKYEILGKT
                     RDDASGEAFDKISRAMNLGYPGGPIIDNLAKNGNKHAIEFPRAYLEEDSYDFSFSGLK
                     SSVLNYLNGKRMKNEEIVVEDVAASFQEAVVEVLSNKALKAVKDKGYNIITLSGGVAS
                     NSGLRAKITELAKDNGITVKYPPLILCTDNAAMIGCAGYYNFINGKTHDMSLNAVPNL
                     KINQ"
     gene            4122..6830
                     /gene="hpdB"
                     /locus_tag="PC700_002452"
     CDS             4122..6830
                     /gene="hpdB"
                     /locus_tag="PC700_002452"
                     /EC_number="4.1.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase large
                     subunit"
                     /protein_id="HDF2581754.1"
                     /translation="MSQSKEDKIRSILEAKNIKSNFQNKENLSEFNEKKASKRAEDLL
                     DVYYNTLSTADMEFPYWYNREYRKSDGDIPVVRRAKALKAAFSHMTPNIIPGEKIVMQ
                     KTRHYRGSFPMPWVSESFFVAQGEQMREEAKKLASNTADELTKFGSGGGNVTESFGNV
                     VSIAGKFGMRKEEVPVLVKMAKEWVGKSVEDLGFHYEKMMPDYDLKENLMSTLICMFD
                     SGYTLPQGREVINYFYPLNYGLDGIIEMAKECKKAVAGNASGDGLIGMDRLYFYEAVI
                     QVIEGLQTWILNYAKHAKYLESIETDLEAKKEYSDLVEILEHIAHKQPRTFREALQLT
                     YTIHIASVNEDAISGMSIGRFGQILYPWYEQDIEKGLITKEEVIELLELYRIKITCID
                     CFASAGVNGGVLSGNTFNTLSIGGLKEDGSTGANELEELLLEASMRCRTPQPSLTMLY
                     DEKLPEDFLMKAAECTKLGSGYPAWVNNSNGTTFMMKQFADEGMTVEEARAFALGGCL
                     ETSPGCWKQLTLNGKTYSIAGGAGQSAGSGVHFIANPKILELVLMNGKDHRMNIQVFE
                     PHNKPLDTYEEVIEVFKDYYKQAINVLERANNIELDIWRKFDTSIINSLLKPDCLDKG
                     QHIGNMGYRYNATLNVETCGTVTMVNSFAALKKLVYDDKAFTIEEMKDAILNNFGFKD
                     ALEVGNYSMADQVKVDKTGKYDAIYKACLDAPKYGNNDLYADNILKNYEVWLSKVCEE
                     AQSLYAKKMYPCQISVSTHGPQGAATLATPDGRLSGTTYSDGSVSAYAGTDKNGVYAL
                     FESATIWDQAVVQNSQMNLKLHPTTIKGQQGTKKLLDLTRSYLRKGGFHIQYNVVDSE
                     TLKDAQKNPDNYRQLMVRVAGFTQYWCELGKPIQDEVIARTEYEGV"
     gene            6834..7091
                     /gene="hpdC"
                     /locus_tag="PC700_002453"
     CDS             6834..7091
                     /gene="hpdC"
                     /locus_tag="PC700_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase small
                     subunit"
                     /protein_id="HDF2581755.1"
                     /translation="MRKHSDCMNFCAVDATKGICRLSKQMINLDDSACPEIKVMPKCK
                     NCKNFVEANDEGIGKCVGLEKEDWVYSTLNAITCEGHVFNE"
     gene            7084..8034
                     /gene="hpdA"
                     /locus_tag="PC700_002454"
     CDS             7084..8034
                     /gene="hpdA"
                     /locus_tag="PC700_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase activase"
                     /protein_id="HDF2581756.1"
                     /translation="MSSQKQLEGMIFDVQSFSVHDGPGCRTTVFLNGCPLSCKWCANP
                     ESWTVRPHMMFSELSCQYENGCTVCHGKCKNGALSFNLDNKPVIDWNICKDCESFECV
                     NSCYYNAFKLCAKPYTVDELVQVIKRDSNNWRSNGGVTFSGGEPLLQHEFLHEVLLKC
                     HEVNIHTAIETSACVSNEVFNKIFKDIDFAFIDIKHMDREKHKEQTGVYNDLILENIS
                     NLANSDWNGRLVLRVPVISGFNDSAENISDIISFMHKNNLIEINLLPFHRLGESKWIQ
                     LGKEYEYSDKGDIDEEHLEELQDIFLDNGIACYVGHETAF"
     gene            8354..9316
                     /locus_tag="PC700_002455"
     CDS             8354..9316
                     /locus_tag="PC700_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2581757.1"
                     /translation="MNKSWSNLIIKNKDKKVRSGWIIITVMAAFYIFEYYLSTILMEI
                     IRKILIATGDINLKTNYFSEYANWLNDVGLQVVFQILTDSLMIIIPIIVWRFIMKNPI
                     SEMGLHSYRIKKKEGYVGMLLGIFNCTVIFIITITIGGGHVTSWVPKISPLTVSWILV
                     FILVAFAEEILNRGFFMAVLRRCKNIYFIMIVPSVIFGLIHIWNPDVTFLSVINIIII
                     GILFSYMFIKSSNIWMCIGYHFTWNVFQGIIYGMPVSGLQVPGIITTQITHGNLLNGG
                     MFGIEGGILTTFVNLLSFIFVWYYYRDSKYDFVSDKVNVTNIKN"
     gene            complement(9415..>9953)
                     /locus_tag="PC700_002456"
     CDS             complement(9415..>9953)
                     /locus_tag="PC700_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2581758.1"
                     /translation="LLIIYFMISVIVPTSFILILQGAQPNYSGIIKFIFTPITSLTTI
                     YIFFSEEYAWRGFLQNIFFDKFGKKLGVIILGMCWSLWHLPLIFTLYTPEAPILGVIL
                     RSIHIVGISIFLGYLYIKTKNIWFCAIIHVLNNSAFLITNSKESTITYHDILIVSMVV
                     LIFYVPFLFTKEYKNNLE"
BASE COUNT         3899 a         1038 c         1853 g         3163 t
ORIGIN      
        1 taaaaaaata aaaaaattat ttttagatga taaagggtat tattttaata gtaattggga
       61 agatattcat attggtattt atgatacaaa attaggagat tctttaaagt atatcactaa
      121 tgaatatatt gaaaatatgg aaagggtaaa atacgattat cgtgaattaa aaaatataga
      181 aaatcaaact gatggtatgt gtattttagc aataaggcag aattacaagg cattagagta
      241 tgtaaaaaat caaaccgaag aattatgttt agaggcaata aagtataatt ataaagcact
      301 tgaatatgta aaagaacaaa cagaatactt gtgtcttgaa gctataaaaa aagattgtaa
      361 tgcacttaag tatgtacgaa ataaaacaga agggcttatt ataaaagcaa taagtcattc
      421 ttccaatata gatgttgtaa gtatactaaa ggctttagag actcaaacaa gaaggatatg
      481 tttagaagca attaaaaaag atggcaggtg tcttgcatac gttagagaac agagtgaaga
      541 attatgtata gaggccatta agcaaaatta taaagcatta aaatatgtaa aaaatcaaac
      601 ggaaaaaatg tgtatagaat ccgtaagaca aaatggtatg gcattacaat atgtaaataa
      661 acaaactgat aaaatatgta tagaagctgt caaacaagat ggaagaagtc tacaatttgt
      721 caacaataag acagaagaaa tatgtataaa tgctataaga tatttaaata aaaaatataa
      781 tattaaagat gtactaagtt acatagataa gtatacagaa gatatatgta tagagattgt
      841 aagacaaaat ggaaaaatgc ttatgtatat aaaaaatcaa acagaaaaaa tgtgtattga
      901 agcagtaaaa gagaactata agtctcttaa gtatgtaaaa gagcaatctg agagaatatg
      961 taaagaagca ttgaagcaaa accataaagc aaaagagtat gtaaaaattg ctattgatga
     1021 ttgtatataa ggtgtagata cagccatcaa tagtgaaaga taaaaaaaga taaagacaag
     1081 tactttataa taatctaggc ttatactata attaattaag cagtgatata aattcacatg
     1141 acgattatat caaaatggag atgaattgat ggcaaaaata tatttagaaa atgaaaataa
     1201 aactagagaa ataggatata aattaggtaa acttttaaaa gaaggcagtg ttatctgttt
     1261 agtaggagat ttaggagctg gtaaaactac aatgacacaa agcttggcag attcattagg
     1321 gatagaagat tatataacaa gtcctacttt tactataata aatgaatatg aaggaaaaat
     1381 acctttgtat cattttgatg tttatagaat aggtagtagt gatgaaatgt atgatattgg
     1441 ttatgatgaa tatgtaaatt caaatggaat ctgtataata gaatgggcta atctaataga
     1501 agatatactt ccaaaagaat atttgaatat agaattgaga tataaagatg aaggaagaga
     1561 aatgatatta actccaaaag gagagtttta taaagagata gttgaggagt tgattaaata
     1621 atgaaaatac taggtatgga tacgtcttct atggcagcaa gtgtagcagt tgttgaagat
     1681 gataacttaa tatgtgagtt tacagtaaac aacaaaaaaa ctcattcaca aaagcttatg
     1741 ccaatgatag aaaatatgtt gagtatgagt gatttaagta taaaagatat ggatttatta
     1801 gctgtatgta taggtccagg ttcgtttaca ggacttagaa taggtatggc tacagtaaaa
     1861 gctatggccc atgtaaataa tataccaata atagcagtaa attctttgga atctctagca
     1921 aataacataa atttctgtga tagaaagata tgttgtatat tagatgcgca gaaaaatcaa
     1981 gtttattcag ctaaatacaa gtttgaaaat ggagagatga tagaattaga tggtgttgat
     2041 gttgtagagt ttgatgcttt agtagatgaa attgtatcaa ctaatgaaga atttataata
     2101 gttggagaag ctgtatacaa atataaagac aagttagaga atataaaaaa tataaaaata
     2161 ccttcaccag ctaataatgt atctaacgca ggaagcttat gctctttagc attaaataaa
     2221 tataataaaa atatagatgt acatacttgt tatacgataa atcctatgta tatcagaaaa
     2281 tctcaagctg aagtacaata tgatgaaaaa atgaagaggc taaataatgg aagataatac
     2341 aaaactaata gtagatgata taaaaataga agaaatgact acagaagata tagatgaagt
     2401 ttttgaagta gaaaaaaatt gttttgaaga ttattggtca aaagagtctt ttagaaaaga
     2461 gttgtctaat gaagtagcaa ggtatatagt agctaagtta aatggaaaaa ttgtcggata
     2521 tgtgggaatt tggcttatac ttgatgaagg gcatataaat aatgtggctg ttcatagtga
     2581 ctatagaggg aaaaaaatag gggacaagtt gataaaatgc atagttgatt tatgtaaaga
     2641 taataatatt gcatctatga cattagaagt tagagcatca aataaaatag cacaaaattt
     2701 atatagaaaa tatggattta aaatgggtgg aataagaaaa gaatactaca gtgataataa
     2761 agaagatgca attataatgt ggaaccaatt aaaagaggtg tagagtttat gagtgatata
     2821 ataacattag ctatagagag tagttgtgac gaaacagctg catcagtttt aaaaaatggt
     2881 agagaagtac tttcaaacat aatctctact caaatcgaga cacataaaaa atttggtggt
     2941 gtagttccag aagtggcctc aagaaaacat gtggaaaata ttgatatagt tgttcaagaa
     3001 gctttagaca aagcaaatat aggttttaat gatatagacc atatcgcagt tacatatgga
     3061 cctgggctag taggtgcttt attggttggt ctatcttatg ctaaagcact tgcatatact
     3121 ttgaatattc cacttgtagg agtaaatcat atagaagggc atctaagtgc aaactatata
     3181 gagcataagg atttaaaacc tccttttata acattaatag tatctggagg tcatactcac
     3241 ttggttgaag taaaagatta tggcaagtat gaaattttag gaaaaacaag agatgatgca
     3301 tctggagaag catttgataa gatttcaaga gctatgaatt taggctatcc aggaggtcct
     3361 attattgata atctggccaa gaatggaaat aaacatgcta tagaatttcc aagagcctat
     3421 ttagaagaag atagctatga ttttagtttt agtggattaa aatcttctgt attaaattat
     3481 ctaaatggaa aaagaatgaa aaatgaagaa atagtagttg aagatgtagc agcatctttc
     3541 caagaggctg tagtagaggt tttatctaat aaagcactaa aagcagtaaa agacaaagga
     3601 tacaatatca taactctttc tggtggggtt gcttctaatt cggggcttag agctaaaata
     3661 acagagcttg ctaaagacaa tggtattact gttaagtatc ctcctctaat tttatgcaca
     3721 gataatgcag ctatgatagg gtgtgcagga tattataatt ttataaatgg gaaaacgcat
     3781 gatatgagtt taaatgcagt tccaaattta aagataaatc agtagagtta gatgaatttg
     3841 taaatataaa agtattgttt caaaaaagta agtagaagtg aaattaaata taataaaaat
     3901 aaatatagta tatattaaaa cttgaagtaa aactatttat aatctttata ttccaatgaa
     3961 ttgcaacaaa ttttaggata tacagattgt ctttggctta gatttgcttc aagtttaatt
     4021 ttttctatag aaagattatt ttaaaaagtg ggtaatatac cctttttttt cattcttaaa
     4081 aagttttata ctaattatag aaagattaag gaggggaaga aatgagtcaa agtaaagaag
     4141 acaaaataag aagtattttg gaagcaaaaa atattaaatc aaattttcaa aataaagaaa
     4201 atttaagtga atttaatgaa aaaaaagcaa gtaaaagagc agaagactta ttagatgtat
     4261 attataatac attatctact gctgatatgg aatttccata ttggtataat agagaatata
     4321 gaaaatcaga tggtgatata ccagtagtta gaagagctaa agctcttaaa gctgcttttt
     4381 cacatatgac accaaatata atacctggtg agaaaatagt aatgcaaaag acacgccatt
     4441 atagaggttc ttttccaatg ccatgggtaa gtgaaagttt ctttgttgct caaggagaac
     4501 aaatgagaga agaggcaaag aaattagcca gtaacacagc tgatgaactt acaaaatttg
     4561 ggtctggtgg aggaaatgtt actgaaagtt ttggaaatgt cgtttcaata gctggtaaat
     4621 tcggaatgag aaaagaagaa gtacctgtac ttgtaaaaat ggcaaaggaa tgggttggaa
     4681 agtctgttga agatttaggt tttcactatg aaaaaatgat gccagattat gatttaaaag
     4741 aaaacctaat gagtacactt atatgcatgt ttgattcagg gtatacactt cctcaaggta
     4801 gagaagttat aaattatttt tatcctttaa attatggatt agatggaatt atagaaatgg
     4861 caaaagaatg taaaaaggca gttgcaggaa atgcatctgg agatgggctt attggtatgg
     4921 accgtctata tttttatgag gctgttattc aagttattga aggattacaa acttggattt
     4981 taaattatgc aaaacatgca aaatatttag aaagtataga gacagattta gaagctaaaa
     5041 aagaatactc agatttagtg gagatattag aacatattgc acataaacaa cctcgtacat
     5101 ttagggaagc tcttcagttg acttatacaa ttcatatagc atcagtaaat gaggatgcta
     5161 tatctggtat gtctatagga cgttttggtc aaattttata tccttggtat gaacaagata
     5221 tagaaaaagg actaattaca aaagaagagg ttatcgaatt attagagctt tatagaataa
     5281 aaatcacttg tattgactgt tttgcttcag cgggcgtaaa tggaggagta ctatcaggta
     5341 atacatttaa tactttatct ataggtggat taaaagaaga tggttctaca ggagctaatg
     5401 aactagaaga gttattatta gaagcaagta tgagatgtag aactcctcaa cctagtttaa
     5461 ctatgctata tgatgaaaag cttccagaag atttcttaat gaaagctgct gaatgtacaa
     5521 agcttggttc tggttatcca gcctgggtaa acaactcaaa tggaacaaca tttatgatga
     5581 aacaatttgc tgatgaaggt atgactgttg aagaagcaag agcatttgca ttaggtggat
     5641 gtttggaaac gtctccaggg tgttggaaac agttaacttt aaacggaaaa acgtactcta
     5701 ttgcaggtgg agcaggtcaa tctgctggct ctggagttca ctttatagct aatcccaaaa
     5761 ttttagaact tgttcttatg aatggtaaag accatcgtat gaatatacaa gtatttgagc
     5821 ctcataataa gccattagat acttatgaag aagtcataga agtatttaaa gattattata
     5881 aacaagcaat aaatgtttta gaaagagcaa ataacattga gttagatata tggagaaaat
     5941 ttgatacttc tataataaat tcactactaa agccagactg tttagacaaa ggtcaacata
     6001 taggaaatat gggatatcgt tataatgcaa cattaaacgt agaaacttgt ggtactgtta
     6061 ctatggtcaa ttcatttgca gcacttaaga aacttgtata tgatgacaaa gcatttacaa
     6121 tagaggaaat gaaggatgca atattaaata attttggatt taaagatgca ttagaagtag
     6181 gaaattattc tatggcagac caagtaaaag tagataagac tggtaaatat gatgctattt
     6241 acaaggcctg tcttgatgca cctaaatatg gaaataatga tttgtatgca gataatattc
     6301 ttaaaaacta tgaggtatgg ttatcaaaag tatgtgaaga agcacaatct ttatatgcta
     6361 agaagatgta cccttgccaa atatctgttt ctacacatgg accacaggga gctgctacct
     6421 tggctactcc agatggtaga ctaagtggaa caacatattc agatggttca gtctctgcat
     6481 atgcaggaac agataaaaat ggagtatatg cattatttga atctgcaact atttgggacc
     6541 aagcagtagt tcaaaattct cagatgaatt taaaacttca tccaactact ataaaaggac
     6601 aacaaggaac taaaaaatta ttagatttaa caagaagtta tttaagaaaa ggtgggttcc
     6661 atattcaata taatgttgta gattcagaaa cattaaaaga tgcacaaaaa aatcctgaca
     6721 attatcgtca attaatggta cgtgttgctg ggtttactca atattggtgt gaattaggta
     6781 aaccaataca agatgaagta attgctagaa cagagtatga aggggtgtaa aatatgagaa
     6841 agcatagtga ttgtatgaat ttttgtgcag tagatgcaac caaaggaatt tgtagattat
     6901 caaaacaaat gattaattta gatgattcag catgtccaga gataaaagta atgccaaaat
     6961 gtaaaaattg taaaaatttt gttgaagcta atgatgaagg tataggcaaa tgtgttggcc
     7021 tagagaaaga agactgggta tattcaacat tgaatgcaat tacttgtgaa ggacatgtgt
     7081 ttaatgagta gtcaaaagca attagaaggc atgattttcg atgtacaaag cttttcagtt
     7141 catgatggtc caggttgtag aacaactgta tttttaaatg gatgtccact gagttgtaaa
     7201 tggtgtgcaa atccagaaag ttggactgta agaccacata tgatgtttag tgaattatct
     7261 tgtcaatatg aaaatggatg tactgtatgt catggtaagt gtaaaaatgg tgcattgagc
     7321 tttaatcttg ataataagcc agttatagat tggaacatct gtaaggactg tgaaagtttt
     7381 gaatgcgtca actcatgtta ttataatgcg tttaaattat gtgcaaaacc ttatactgta
     7441 gatgaacttg tgcaggtaat taaacgtgat tctaacaatt ggagaagtaa tggaggagta
     7501 acatttagtg gaggtgagcc actacttcag catgaatttt tacatgaagt attattaaag
     7561 tgtcatgagg taaatataca cactgccatt gaaacaagtg catgtgtatc aaatgaagtt
     7621 tttaataaaa tatttaaaga tattgatttt gcatttattg atataaagca tatggataga
     7681 gaaaaacaca aagaacaaac aggtgtatat aatgatttaa tattagaaaa tatatcaaac
     7741 cttgcaaatt ctgactggaa tggaaggtta gtacttagag taccagttat ctctgggttt
     7801 aatgatagtg ctgaaaatat aagtgatatt atatctttta tgcacaaaaa taatttaata
     7861 gagattaact tattaccatt tcataggcta ggagaatcaa agtggattca attaggtaag
     7921 gagtatgagt attctgataa gggagatatt gatgaagagc atcttgaaga attacaagat
     7981 atatttttgg ataatggtat agcttgctat gttggtcatg agacagcttt ttaaaatcaa
     8041 atacaagttt ttttaattaa aaaaggatgt tacactaaaa gctagagtta catccttttt
     8101 tatgtggttt ttatgtagaa ataattaagt aaagttttat atgttttata tattgttcat
     8161 attgctttat gtccataaat attactttaa atttatactt tatatatttt ttatagttta
     8221 aattacagtt agagaatata ttacgtttac tttattgaag cagaagtttt gtggtaaaat
     8281 actactatga agaaatttaa tagaatatat tttagaaaca actaaaaata atagttagga
     8341 ggaagacatc tatatgaaca aatcatggag taacttgatt attaagaata aagacaaaaa
     8401 agtacgttca gggtggatta ttataactgt tatggctgcc ttttacatat ttgagtatta
     8461 tttaagcact atacttatgg aaataataag aaaaattctg attgctacag gtgacattaa
     8521 tttaaaaaca aactattttt cggaatatgc taattggtta aatgacgttg ggttacaggt
     8581 tgtatttcaa attctaacag attcacttat gattattatt cctataattg tatggagatt
     8641 tattatgaaa aatcctatta gtgaaatggg attacattct tatagaatta agaaaaaaga
     8701 aggatatgta ggaatgttat taggaatatt taattgtaca gtaatcttta ttataacaat
     8761 aacaattggt ggtggtcatg ttacttcatg ggtaccaaaa atttcacctt taacagtttc
     8821 ttggatactt gtctttattt tagttgcttt tgcagaagaa attttaaatc gtggtttttt
     8881 tatggcagtt ttacgaagat gtaaaaatat ttattttata atgattgttc catctgttat
     8941 ttttgggttg attcatatat ggaatcctga tgttactttt ttgtctgtaa ttaatattat
     9001 aattattggg attctttttt catatatgtt tataaagtcg tcgaatatat ggatgtgtat
     9061 aggttatcat tttacatgga atgtatttca ggggattata tatggtatgc ctgtaagtgg
     9121 acttcaggtt ccagggatta taactacaca aatcactcat ggtaatctat taaatggagg
     9181 gatgtttggt attgaaggtg gaattttgac tacttttgtt aatctactta gctttatatt
     9241 tgtatggtat tactatagag attctaagta tgattttgtt agtgataaag taaatgtgac
     9301 taatatcaag aattagttga ttataataaa ttttaataat atcttctaaa cttaaagtat
     9361 tctgtaggat tgttggaaat ttacaaaaat ataaaaataa cttggatatt ctatttactc
     9421 caagttattt ttatattctt ttgtaaatag aaatggaaca taaaaaatta aaacaaccat
     9481 agatactatt agtatatcat ggtaagtgat agttgattct ttagaatttg tgataagaaa
     9541 agcactatta tttaaaacat gaattatagc acaaaaccag atattcttag tttttatata
     9601 aagatatcct aaaaatattg aaattccaac tatatgtatt gaacgtaata ttactcctaa
     9661 tattggcgct tcaggtgtat ataatgtaaa tattagtggt aagtgccaca agctccaaca
     9721 catacctaat attatgactc ctaatttttt accaaattta tcaaaaaaaa tattctgtaa
     9781 aaaacctctc caggcatatt cttcactaaa aaaaatataa atagtagtta atgatgttat
     9841 tggagtaaat ataaatttta tgataccact ataattaggt tgagcccctt gtagtattag
     9901 tataaatgat gttggaacaa ttacagaaat cataaagtaa ataattaata aag
//
LOCUS       DAMJWP010000125         9948 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.130, whole genome shotgun sequence.
ACCESSION   DAMJWP010000125 DAMJWP010000000
VERSION     DAMJWP010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9948
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.130"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(109..1431)
                     /locus_tag="PC700_002457"
     CDS             complement(109..1431)
                     /locus_tag="PC700_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HDF2581759.1"
                     /translation="MKILLVDDELEYGVVMKKILQKKGYLVDIALSGEEAINIIKKDK
                     NYDLVLSDVMMKNMDGVQLLDRIKSINRDIEVILVTGYGSIENAVDAMKRGALSYFIK
                     SNPIENLLEEVEKVKASKISVSVQKNNFEFTLESKNRDFNNIIKIAKKAACKDVNILI
                     LGESGVGKDILARYIHSISPRKNEIFVPVNCCSFSENLLESELFGHEKGSFTGAVDSR
                     KGRFELSNKGTLFLDEIGDIPLNVQVKLLRTLEDKSIERIGSNKSIKVDFRLICAMNK
                     EPKVEISNGNIREDFFYRISTITITIPPLRKRREDLTTLIEFFLNKYQIEHDKKIHSI
                     DKEVKDFLLNYNYPGNIRELKNIINRLVVLSEEGNLSKDNLNLISNNVYIDDKISIRP
                     LREIRKEFECEYIEKVLSLCGNNISNTAKKLEISRRQLTNKISEYNIK"
     gene            complement(1431..3425)
                     /locus_tag="PC700_002458"
     CDS             complement(1431..3425)
                     /locus_tag="PC700_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDF2581760.1"
                     /translation="MNKKKIVIIGIIYSFLVVFSLTNMYVNMEYNLNVFEYIKKSLPF
                     TEEEKKWLEKHKNLIYSSDQSSPPLRYKGKEDGQYKGIIVDLINSLSIQIGRDFYFKP
                     NNWWKESFVNSIDDSIKFFDLIPSKERANKFIFTDPIYTLSANILKDKKSQDINSYMD
                     LKGKTVAIPEGDYSINFLKQKIQDINILLTPDIKTGVNHLMSGKVDAVVGDEPVLRYY
                     INNYGLSNKYSVLSNPIYTKKAVLAVPKQYEELVSILNKGIFKLQKNGVYKDLKKKWY
                     STYNEVDDILYERGIVPSIYLFIGIILISIYVFYSYTYLLKIEIKRKTEQVIENKKTL
                     EATFNSITDIIMLVDENNNIVESNKVLYDFMGEMSYKIADLISMIKGVIKNTFSENTN
                     KTSEIEIYNKILKINTFPVEYKKNNTEYIVVLIKDITNEKIVEAKLLRENKMISIGQL
                     ASGVAHEIRNPLGIIRNNCYLLKDNVTMEEVNDCVKSIESNVDRASNIITNLLNFARI
                     SDDNLEHINIRNFIENIVKLQYKMLQLKNVEIKIDCEHNLVCYINGESLKHVFINLIS
                     NSIDAIHQDGKIIIYCYEKNHCLFIDFKDNGEGIKEDALKDIFNPFYTTKPIGEGTGL
                     GLYITYNEIKKNNGDISVESKLGVGTCFHIKIPLNKEVTI"
     gene            complement(3512..4759)
                     /locus_tag="PC700_002459"
     CDS             complement(3512..4759)
                     /locus_tag="PC700_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDF2581761.1"
                     /translation="MQITTFGAIFGLLIAIILIIKKFQAVYSLMLGAFIGGLVGGANI
                     TQTVDFMANGAMNISPSILRALASGVLAGSLIKTGAVDKISEQIVKIFGEKRALFSIA
                     ISTMVLAGVGVNLDVSIITVAPIGLYIGRKLNYSKLSILLAMLGGGKAGNIISPNPNT
                     IAVADNFSVNLSSVMMANIIPAIIGVVITVILASILINKGNKVQSYEILEQREDLPSL
                     FKSLCGPIIAIFLLFLGNVSPIVIDPMIALPIGGIVTLIVTGNLNNSREYLAFGLSKM
                     QGVCILLLGTGTIAGIIQMSELQQSTIGALQFLNMPQFLLAPVSGILMSLATASSTAG
                     ATIASSTFHDAIINGGLSPISGASIVNAGSSVFEQLPHGSLFHTSSGSINMDIGERFK
                     LIPYEALIGIVMTIISTSIQLVL"
     gene            4987..5532
                     /locus_tag="PC700_002460"
     CDS             4987..5532
                     /locus_tag="PC700_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2581762.1"
                     /translation="MNDLNLGEKIAEVRKKQNLSIRDLAKLADVTPSLLSQIERGLAN
                     PSVNSLKSIASSLNVPLFTFFVSEVDKKNLIVRHDNRKKVILPGSKEVIYEILIPDSS
                     GNLEFAIMDLAPNTSSCIDRITHNGDEIAYVLEGEVKLFMDDDEFTLSKGDSVKVPLG
                     TKHKWQNDSNSESKVIFAVIL"
     gene            5546..6232
                     /locus_tag="PC700_002461"
     CDS             5546..6232
                     /locus_tag="PC700_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HDF2581763.1"
                     /translation="MKTYKFIFFDADDTLFDFKKSESHAFKKLLSEFDLEFNFENYIE
                     SYRNISDKLWLDLEKNIITLNELKLLRFELFANKISLDVDSETLSKMYLNFLGECTFL
                     IPGAIDILQYLKKKYTIVIITNGISAVQKKRLENSKIKGYIDGMVVSEELKISKPNPE
                     IFKYALKKFNCHDKSSALMIGNSLTSDVLGGINSGIDTCWLNSNNSINYTNHIPTYEI
                     NTLIELKKLL"
     gene            complement(6356..6976)
                     /locus_tag="PC700_002462"
     CDS             complement(6356..6976)
                     /locus_tag="PC700_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581764.1"
                     /translation="MNYNSNKNKYLLKDINLEDIFIDKNIVLKFLGYGNRKAPDTISE
                     IVEREIRNINDILDIKIYISEVDINKTPKECKKAFAVLYTIGDKIDIKMNDYMENCNM
                     MAGIALDKIGIVCLDYINEKIKMYLKGKYDSLKISHEIYPGDKDFEVERQKDIYNYVK
                     NKYNSIEIEINDYHQLSPIKSVAMLILMGDEENLESRCSKCPRKCF"
     gene            complement(6966..7988)
                     /locus_tag="PC700_002463"
     CDS             complement(6966..7988)
                     /locus_tag="PC700_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HDF2581765.1"
                     /translation="MFSDMTLLQYINSEDRGFFLPDMGTNGLFLINKKAYEVYDSVDL
                     QLELAKTMDKYFESDFIYSFCDGITFCETLGLETLRPDYDFPSVLTHTITDRDKLRKL
                     DVPDPYTSGRMPLNIESLSVISKSIKKPLYESIQGPFTLAGQLAGATQLLRCIITDKK
                     FVEELLEFTTELVRRYAVAANKAGAKYISIAEPTSVTLSKDRFDEYIVKNLNKIYNEL
                     DCWKGMHICGDTRELLDNMLSCNIDAVSLDQILDYEEIAPMIPRDIVLIGNLDPIKLL
                     GRSKPDKIRRETLKLLKKMRGYDNFLCDFGCNCLNTTPVENLQAAIKAGRINYKELDR
                     IDIDEL"
     gene            complement(7989..8627)
                     /locus_tag="PC700_002464"
     CDS             complement(7989..8627)
                     /locus_tag="PC700_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423639.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HDF2581766.1"
                     /translation="MDILDDIAECILNMGVDNIENLVKIAIDKNIDVEDIYEYGLNKG
                     MIGALDKFENKEYYLSEVIVCTDALNKGINLLKGTGKVKKKSKGVILMSVVEGDTHEI
                     GKNIVKVMVEATGYKVIDLGVNRKSEDIIEEAIKNNVDIIGLSSMMTTTMENMKSVID
                     ELNMIEIDKRPKVIIGGGPVSMEFAEEIGADGYSSNAPKAVKLINKLIGGEV"
     gene            complement(8745..9896)
                     /locus_tag="PC700_002465"
     CDS             complement(8745..9896)
                     /locus_tag="PC700_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004452985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2581767.1"
                     /translation="MELRPRSAKLEEFDKVIELINYVFRISRNHKPTMMEEFPLLLSK
                     NNIENMIIISEDDKVVSDVNYLIQDVSIQGNRLKVAAIGGVCTHPDYEKRGYSSKILD
                     KVEEKMFYDGVDIVIISGTRSLYSRRNCSLVKSFYKYTIKPEDVKIAYEIVEFDETNF
                     EKDDDLDKMLELYNQNSTRFIRTRDEFQKLLHAATIAWGPIGYKKVFIKENNNIIGYL
                     IIRTIKKEDSTVGEVAEIGLNSVNVENILKYVANKFGLEYLNYKVHVKNLKDQLKCNG
                     TESLDYQQGTMKIINFTKLCDSLRSYFSQYVDFELLKYMEFKQVENKYIIKYKEEELV
                     IENLDKLNKLFFEKNEEQYNEFKHLKNIYEFATKAFPVDFPWTANLNYQ"
BASE COUNT         3275 a         1659 c          983 g         4031 t
ORIGIN      
        1 aaatattaat ttttaaaatt ataataaaaa aagataaaca gattttttct ctttatcttt
       61 taatacagtt tgagaaattt ttttctcact taatgagaag tttttttctc acttaatatt
      121 atactctgat attttattgg ttaactgtct tctactaatc tccaattttt tagctgtatt
      181 tgaaatatta tttccacaca aagataaaac tttttcaata tactcacatt caaactcttt
      241 tcttatttct cttaaaggtc taatacttat tttatcatct atgtatacat tatttgatat
      301 taaatttaaa ttgtcttttg ataaattgcc ttcttcactt aaaactacaa gtctatttat
      361 tatatttttt aattctctta tatttcctgg ataattgtaa tttaataaaa aatcctttac
      421 ttccttatca atcgagtgta tctttttatc atgttcaatt tggtacttat ttaagaaaaa
      481 ttctataaga gtagttaagt cttctcttct ctttcttagt ggtggtatag ttattgttat
      541 tgtacttatt ctataaaaaa aatcttctct tatatttcca tttgatattt ccacttttgg
      601 ttctttattc atagcacaaa ttaacctaaa gtcaacttta atagatttat tacttcctat
      661 cctctctata gatttgtcct caagtgtcct caaaagtttt acctgaacat ttagaggtat
      721 atctcctatt tcatccaaaa atagagttcc tttattcgaa agttcaaatc taccttttct
      781 actatctact gctcctgtaa aagaaccttt ttcatgacca aacaactcag actccaatag
      841 attttctgaa aaagaacaac aatttacagg tacaaatatc tcattctttc ttggacttat
      901 actatgtata tatctagcta atatatcttt tcctacacca gattccccca agattaatat
      961 attaacatct ttacaagcag ctttttttgc tatttttatg atattattaa aatctctatt
     1021 tttagattcc aaagtaaatt caaaattatt tttttgtaca gaaacagata ttttacttgc
     1081 ctttaccttt tcaacttctt ctaataaatt ttcaatggga ttacttttta taaaataaga
     1141 taaagctcct cttttcatag catcaactgc attttcaata cttccatatc ctgtaactaa
     1201 tataacctct atatctctat taatagattt aattctatct aatagctgta ctccatccat
     1261 atttttcatc ataacatcag ataaaaccaa atcataattt ttatcttttt taattatatt
     1321 tatagcttct tcaccactta gtgctatatc tacaagatag cccttctttt gtagaatttt
     1381 tttcattacc actccatatt ctagctcatc atcaactaat agtattttca ttatatagtc
     1441 acctctttat ttaatggaat ttttatatga aaacaagttc caacccccaa tttactttca
     1501 acacttatat ctccattatt ctttttaatc tcattataag taatatataa acctaaacca
     1561 gttccctcac caattggttt tgttgtatag aagggattaa atatgtcctt tagggcatct
     1621 tcttttattc cttcaccatt atctttaaaa tctataaata aacaatgatt tttttcataa
     1681 cagtatataa taatttttcc atcttgatgt attgcatcta ttgaattaga aattaaattt
     1741 ataaatacat gttttaacga ttctccattt atatagcata ctaaattatg ttcacagtca
     1801 atttttattt caacattctt taactgtaac attttatatt gaagtttcac tatattttca
     1861 ataaaatttc ttatatttat atgttctaaa ttatcatctg atattcttgc gaaatttaac
     1921 aagttggtta ttatattact tgccctatct acattacttt ctattgattt aacacaatca
     1981 ttcacttctt ccattgtaac gttatctttt aatagatagc aattatttct tattataccc
     2041 aaaggatttc ttatctcatg tgctacccca gaagctaatt gtcctataga tatcattttg
     2101 ttttctctaa gtaatttcgc ctctactatt ttttcattag ttatatcttt aataagaact
     2161 actatatact cagtattatt ttttttatac tctacaggaa aagtgtttat ctttaaaatt
     2221 ttattgtata tttctatttc actagtttta ttagtatttt cagagaaggt atttttaata
     2281 acacctttta tcatgctaat caaatctgct atcttataac tcatttcacc cataaagtca
     2341 tatagtactt tgttcgattc tactatattg ttattttcat ctacaagcat tattatatct
     2401 gtaatactat taaaagttgc ctctaaagtt tttttatttt ctattacctg ttctgttttc
     2461 cttttgattt ctatctttaa caaatatgta tagctataaa aaacataaat tgatattaaa
     2521 attataccta taaaaagata aatactaggc acaattcctc gctcatacaa tatatcatca
     2581 acttcattat acgtagaata ccattttttc tttaaatctt tatacactcc atttttctga
     2641 agtttaaaaa tgcctttatt taaaatacta accagctctt catattgttt aggtacagca
     2701 agaactgctt ttttagtata tataggatta cttaaaacac tgtatttatt tgaaagtcca
     2761 taattattta tatagtatcg caatactggt tcatcaccaa caactgcgtc tacttttcca
     2821 gacattaaat ggttgactcc agtctttata tctggagtca ataaaatgtt tatatcttga
     2881 attttttgct ttaaaaaatt tatagaataa tcaccttctg gaattgctac tgttttcccc
     2941 tttaaatcca tgtaactatt tatatcttgg gactttttat ctttcaatat atttgcactc
     3001 aaagtgtata ttgggtcagt aaatataaat ttatttgccc tttcttttga tggaatcaag
     3061 tcaaaaaact ttatactatc atctattgaa tttacgaaag attctttcca ccaattattt
     3121 ggtttaaaat aaaaatctct accaatttga attgacagag aatttattaa atcaacaata
     3181 attcccttat actgtccatc ttccttaccc ttatatctca aaggaggtga actttggtca
     3241 gagctgtaaa tcaaattctt gtgtttttct aaccactttt tttcttcttc tgtaaatgga
     3301 agtgacttct taatatattc aaatacattt aaattatact ccatatttac atacatattt
     3361 gtaagtgaaa atactactaa aaatgaataa ataatcccta taataacaat ctttttttta
     3421 ttcactttaa aactccaatc ttctttttta ctataataaa ttcaatattt aataacaaaa
     3481 tcctactttt ttcattaaat atttatattt attataaaac taattgaata gatgttgaaa
     3541 ttatagtcat tactattcct atcaatgctt catatggaat taacttaaat ctttcaccta
     3601 tatccatgtt tatactacct gaactagtat gaaatagtga gccatgtggt aattgctcga
     3661 atacactaga cccagcattt actattgatg cacctgaaat cggtgatagc ccaccattta
     3721 ttatagcatc atgaaaagta gatgatgcta tagtagcacc agctgttgat gatgctgttg
     3781 ctaatgacat aagtataccc gaaactggtg ccaataaaaa ttgtggcata tttaaaaatt
     3841 gtaaagcacc tattgtactc tgttgtaatt cagacatctg tataattcct gcgatagtcc
     3901 ctgttccaag taataatatg catacccctt gcatcttaga taatccaaaa gctaaatatt
     3961 ctcttgaatt gttcaagttt cctgtgacaa ttagagttac gattcctcct attggaaggg
     4021 ctatcattgg gtctataact ataggagata catttcctaa aaacaataga aatatagcta
     4081 taattggacc acataatgat ttaaataaac ttggtaaatc ttctctttgc tctaaaattt
     4141 catatgattg tactttattt cctttattta ttaatatact tgctaaaata actgtaataa
     4201 caactccaat aattgctgga attatatttg ccatcataac actagagaga ttgacagaaa
     4261 aattatctgc tactgctata gtatttggat ttggtgatat tatattacct gctttacccc
     4321 ctcccaacat agctaatagt attgacaatt tagaataatt taattttcta cctatatata
     4381 atcctatagg tgctacagtt attatagata catccaaatt tacgcctact ccagcaagta
     4441 ccatagttga aatggcaatc gaaaataaag ccctcttttc accaaatatt tttacaatct
     4501 gttctgatat tttatctact gctcctgttt ttattaaact tccagctaat acacctgatg
     4561 ccaaagctct aagaattgat ggtgatatat tcatagctcc atttgccata aaatctacag
     4621 tttgtgttat atttgctcct ccaactaatc ctccaataaa tgctcctaac attaaactat
     4681 atacagcttg aaattttttt ataattaaaa taattgctat taataaacca aatatagctc
     4741 caaaagtcgt tatttgcata atgtcccctc ctatcataaa ttaactatct tttgcttaat
     4801 tttatttaaa tatattttaa gtattctgta tccactttgt caattatatt ttaatttatt
     4861 taattatatt gaacatagtt ttttcaaaac ctaattctat atgttataat aatttcatac
     4921 tacataatca ttttacttga ttattttaag ttaatatttt ttaaattaaa aagagaggat
     4981 atttatatga atgatttaaa tttaggggaa aaaatagctg aagttcgaaa aaaacaaaac
     5041 ttaagtattc gagatttagc aaaactagct gatgttacac catcattatt gagccaaata
     5101 gaacgaggtc ttgctaatcc atctgtaaat tcattaaaat ctatagcctc ttctctaaac
     5161 gttcctttat ttacattttt tgtatctgaa gtagataaaa aaaacttgat agtaagacat
     5221 gataatagaa aaaaagttat tttacctgga agcaaagaag ttatatatga gattttaatt
     5281 ccagactcta gtggtaattt agaatttgca attatggatt tagctccaaa tacttcttct
     5341 tgtattgata ggattactca taatggagat gaaattgcat atgttttgga gggagaagtt
     5401 aaacttttta tggatgatga tgaatttact ttgtccaaag gagatagtgt aaaagttcct
     5461 ttaggaacaa agcataaatg gcaaaatgac tcaaatagtg aaagtaaagt aatttttgca
     5521 gtcatcttgt aaaggagtaa aaaccatgaa gacatataaa tttatatttt ttgatgcaga
     5581 tgatacttta tttgatttta agaaaagtga atcacatgct tttaaaaaac ttctatctga
     5641 gtttgattta gaatttaatt ttgagaatta tatagagtca tatagaaata taagtgataa
     5701 gctttggctc gaccttgaaa aaaatattat aactttgaat gaacttaaat tacttagatt
     5761 tgaacttttt gcaaataaaa tatctttgga tgttgattct gaaactctta gcaaaatgta
     5821 cttaaatttt ttaggtgaat gtacattttt aattccaggt gcaatcgata tattacaata
     5881 tcttaaaaaa aagtatacta ttgttataat aacaaatgga atttctgcag ttcaaaagaa
     5941 aagacttgaa aattcaaaaa taaaaggata tattgatgga atggttgtat ctgaagagtt
     6001 gaaaatatca aaaccaaatc ctgaaatatt taaatacgct ctcaaaaagt ttaattgtca
     6061 tgataaatct tctgccttaa tgattgggaa tagcttaacc tctgatgtct taggtggaat
     6121 aaattcagga atagatactt gttggttaaa ttccaataac agtataaatt atactaatca
     6181 tataccaact tatgaaataa atacacttat tgaattaaag aaactactat agtttgtaag
     6241 tctgttcaaa ttaaatagaa tattactttg caataatttt tttagtttat acataaaatg
     6301 accaaaaaaa ttattgcaat tcatacacaa aacaaatctt agtcttgaaa actagttaaa
     6361 agcattttct aggacatttg ctacatcttg attctaaatt ttcttcatct cccattaaaa
     6421 taagcattgc aacacttttt atgggggata gttgatgata atcattgatt tctatttcta
     6481 tactattata tttatttttt acatagttat aaatatcctt ttgtctctct acttcaaaat
     6541 ctttatctcc tggatatatt tcatgcgata ttttaagact atcatacttt ccttttaaat
     6601 acatttttat tttctcatta atataatcta aacatactat acctatttta tccaatgcta
     6661 tccctgccat catattgcaa ttttccatat agtcattcat cttaatatca attttatccc
     6721 ctattgtata tagtacagca aatgcttttt tacattcttt aggtgtttta ttaatatcaa
     6781 cttcacttat atatatcttg atatctaaaa tatcatttat atttcttatt tctctttcaa
     6841 cgatttcact aattgtatct ggagcttttc tatttccata ccccaaaaac tttagcacaa
     6901 tattcttatc tataaaaata tcctctaaat ttatatcctt taaaagatat ttatttttat
     6961 tactattata attcatcaat atcaatccta tctaattctt tataattaat tcttcctgct
     7021 tttatagctg cttgtaaatt ttctactgga gtggtattta agcagttaca gccaaaatca
     7081 cataaaaagt tatcatatcc tctcatcttc tttaacaatt ttagtgtctc tcttctaatc
     7141 ttatctggct tagaacgtcc aagaagtttt attgggtcta aattaccaat caacactata
     7201 tctctaggaa tcattggagc aatttcttca tagtctaaaa tttggtctaa acttactgca
     7261 tctatattac aacttagcat attatctaat aattctctgg tgtctccaca tatatgcata
     7321 cctttccaac aatcaagttc attgtagatt ttatttaaat tttttactat atattcatca
     7381 aatctatctt tacttagggt tactgacgtt ggctctgcta ttgaaatata ttttgctcct
     7441 gctttatttg cagctacagc atatcttctt accaattcag ttgtaaattc caacaattct
     7501 tctacaaatt ttttatcagt tattatacat cttaaaagtt gagtagctcc agctaattga
     7561 cctgcaagtg taaatggacc ttgtatcgat tcatatagtg gttttttaat actttttgat
     7621 ataactgata agctctctat attgagtggc atacgtccac tcgtataagg gtctggaaca
     7681 tctaattttc ttaatttatc tctgtcagtt atagtatgtg tcaatacact tggaaaatca
     7741 taatctggtc ttaatgtctc taaacccaga gtttcacaaa atgtaatccc atcacagaat
     7801 gagtatataa aatctgattc aaaatacttg tccatagtct ttgctaattc taattgcaaa
     7861 tctacactat catatacttc atatgccttt ttatttatta agaacaatcc atttgtaccc
     7921 atatcaggca gaaaaaaacc tctatcttca ctatttatat actgaagtaa agtcatatct
     7981 gaaaacattt aaacttcacc tcctataagt ttgtttatta atttaactgc ttttggagca
     8041 tttgaactgt atccatctgc tcctatttct tctgcaaact ccatacttac aggtcctcca
     8101 ccaattatta cttttggtct tttatctatc tcaatcatat taagctcgtc tataaccgat
     8161 ttcatatttt ccattgtagt tgtcatcatt gaagataagc ctattatatc tacattgttt
     8221 tttatagctt cttctattat atcttcactt tttctattta cacctaagtc aataacctta
     8281 tatccagtgg cttctaccat tacttttaca atattttttc ctatctcatg agtatctcct
     8341 tctactacag acatcagaat tactcccttt gattttttct ttacttttcc agttcccttc
     8401 aaaagattaa tacctttatt taatgcatct gtgcatacta taacttctga aagatagtat
     8461 tccttattct caaacttatc taaagctcct atcatacctt tgtttaaacc atattcataa
     8521 atatcttcca catcaatatt tttatctata gcaattttta ctaaattctc tatattatct
     8581 acacccatat ttaatataca ttctgctata tcatcaagta tgtccatata atactcatcc
     8641 tttctaatta cttacaaaat tgctttttca atatccaata atttctctta tttaacaagg
     8701 actaactcta ggtatcaatc aattttattt aatatcatct aaaactattg ataatttaaa
     8761 tttgcagtcc atggaaaatc tacaggaaat gcctttgtag caaattcata tatatttttt
     8821 agatgtttaa attcattata ctgttcctca tttttttcaa aaaacagttt attaagctta
     8881 tccaaatttt ctattacaag ttcttcttct ttgtatttaa taatatattt attttcaact
     8941 tgtttaaatt ccatgtattt tagtaattca aaatctacat attgactaaa ataacttctt
     9001 aaactatcac acaacttagt aaaatttatt attttcatag ttccttgttg atagtccaaa
     9061 gattctgtcc cattacattt caactggtct ttaagatttt tcacatgtac tttataattt
     9121 aaatattcta atccaaattt atttgcaaca tatttcaata tattttcaac atttacagag
     9181 tttaacccta tttcagcaac ttctcctaca gtagaatctt ctttttttat tgttcttatt
     9241 atcaaatatc ctattatatt attattttcc tttataaata ccttcttgta accaattggt
     9301 ccccaggcaa tagttgctgc atgcaatagt ttttgaaact catctctcgt tcttataaat
     9361 ctagtactat tttgattata tagttcaagc attttatcta aatcatcatc tttttcaaaa
     9421 ttagtttcat caaattcaac tatttcatat gctattttta catcttcagg ttttattgta
     9481 tatttataaa aactctttac caaactacaa tttcttctac tgtatagact tcttgttcct
     9541 gatataatta caatatctac accatcataa aacatttttt cttctacttt atccaatatc
     9601 ttagatgaat agcctctctt ttcataatct ggatgagtac atactccacc tatagctgca
     9661 accttcaatc tatttccttg aatagataca tcttgaatta gatagtttac atcagaaacc
     9721 actttatcat cttctgatat tataatcata ttttctatat tatttttact tagtaaaaga
     9781 ggaaattctt ccatcatagt tggcttatga tttcttgata ttctaaatac atagtttatt
     9841 aattctatta ctttatcaaa ttcttctaat tttgcacttc ttggtcttaa ttccattcat
     9901 attcctccaa atattattta agatacttaa aactctatta tctataat
//
LOCUS       DAMJWP010000126         9935 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.131, whole genome shotgun sequence.
ACCESSION   DAMJWP010000126 DAMJWP010000000
VERSION     DAMJWP010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9935
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.131"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            338..949
                     /locus_tag="PC700_002466"
     CDS             338..949
                     /locus_tag="PC700_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581768.1"
                     /translation="MNIKRINLLLSTVMAAVIISLPLAVTANAMEKTESDNNVKIELS
                     DKEVEELHNYWPEDKIFSDEKIDEILIKTGQFTQKELDKINQEVDDSAKKVNQNSRVK
                     YINGYNKYRTFTKNGKNYLYIYVSGKTLKKIKAGTDLASTFGGFLPKIWVELDVVAIG
                     KIITINMDGINTNYGIVLSYIEDKWNFQGATYTYRYDHWFYQT"
     gene            977..1159
                     /locus_tag="PC700_002467"
     CDS             977..1159
                     /locus_tag="PC700_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581769.1"
                     /translation="MKKRLYKVFLKTIPNLIIVILSLALSFYTNTFDIDLKILLLIPL
                     AYVLLFIYYYIKTKKD"
     gene            1399..1941
                     /locus_tag="PC700_002468"
     CDS             1399..1941
                     /locus_tag="PC700_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2581770.1"
                     /translation="MLIYLAILELEEDKIKFEKVYEKYRQIMFYVANKILKDDHLSED
                     AVHNAFLRIIKNIDKIDEVDSPRTKAFIVIIVERIAIDFYRKRKREKVSDIEEEYKNR
                     EINFSIEDKVCESNLAIALAKLNESYFQVLSLKFQYGFSNKEIANALNLGEENVYKRI
                     QRARKKLKEILEEMEVGNIE"
     gene            1973..2635
                     /locus_tag="PC700_002469"
     CDS             1973..2635
                     /locus_tag="PC700_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4367 domain-containing protein"
                     /protein_id="HDF2581771.1"
                     /translation="MLRESIPIATKIIIDDLLSDDELNHVFSKGFERKMKKLIKQQKR
                     SSLTNKIIFYSKQTAIVFLIVLASLLTITMSSEALRSRFFRLVIRVYEDLTSFVFSTN
                     GEDNLSLKNKEPEYIPKGFKEVDRVDDFVITYRNDENIEIQYVTDKISSNSIILDTEN
                     AKVKDIFVNGHKAKYIVKGTFLQLFWNDNNFIYLLNVDYQNSGESEFYEDILIKIAKS
                     IK"
     gene            2825..3142
                     /locus_tag="PC700_002470"
     CDS             2825..3142
                     /locus_tag="PC700_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581772.1"
                     /translation="MKSDLYLCKSNKLYRDNEVLEKFMEDLSFLIDDYKFSLETISIL
                     CKINIEKLTNFYAGKGYILYEEMSLIESVLAPFLSAIDIAKHNYDVVIKSRESDKPLI
                     FEN"
     gene            3164..3607
                     /locus_tag="PC700_002471"
     CDS             3164..3607
                     /locus_tag="PC700_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581773.1"
                     /translation="MKRLFALLLTLSLVILPSTLVFANEKAENDSIYAEAPLVFQSNE
                     KAYTHGSGGTAYIGYMSSKHLNWRITSSKGIVILFSGTVEIRNSKNMLVASYPISGGG
                     SSTISGQIDVKYLKKGDYKAILKGTSTTGGGVLVVKSGLVQMFTI"
     gene            3825..4268
                     /locus_tag="PC700_002472"
     CDS             3825..4268
                     /locus_tag="PC700_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581774.1"
                     /translation="MNLRKTVISGMCALVCSSAVAIPAFADEYQDKNIEQYSAVNYID
                     DTMVKMSYIKSGTCNLSISGSTAKVTCFVRGASNVTSTSIIARLQKSNDNGKTWSTIQ
                     TWNASGNISCSLSKSKTIGKGYYRVHSTIKANSESKNIISSTKKY"
     gene            complement(4399..4819)
                     /locus_tag="PC700_002473"
                     /pseudo
     CDS             complement(4399..4819)
                     /locus_tag="PC700_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894307.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            complement(4797..6074)
                     /locus_tag="PC700_002474"
                     /pseudo
     CDS             complement(4797..6074)
                     /locus_tag="PC700_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899679.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(6097..6639)
                     /locus_tag="PC700_002475"
     CDS             complement(6097..6639)
                     /locus_tag="PC700_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581775.1"
                     /translation="MLLCDPASAGGSKHDYSAFLVGSESENGLLCGRLAELAKINART
                     DFDKYILHMIYLLKVYPDITHVYIEKNTFNGTAANQLELKIKNDDVLYYRDIEIINEH
                     QKKNKDDKISTLIPVLNKGQMIFAEEDKAFIQQILNFTGQKYSLHDDAPDISAEFINR
                     IFNIKVNESITLLDRRNLGL"
     gene            complement(6679..6897)
                     /locus_tag="PC700_002476"
     CDS             complement(6679..6897)
                     /locus_tag="PC700_002476"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581776.1"
                     /translation="MLFDFKNTNHLEDAKEFYWENEKEIKFPILWPSFWNCLDMALSY
                     YENPTSFKQEVQNDVNSIGEKWFKTIRT"
     gene            complement(7093..7836)
                     /locus_tag="PC700_002477"
     CDS             complement(7093..7836)
                     /locus_tag="PC700_002477"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581777.1"
                     /translation="MDNLERNIQLIFSYMAKTFIRNGATIREAEKEANEIIKTNSSNL
                     WGVNGLAYQLGKINLEFFCMYFIQDTYLPKEDNVAAPIAKVHHELWEDIQESIIGDGS
                     QQLGRIYPRGTGKSAFGDLATTVWSHCYKHKTYTLICSDIGSTAEKFVKDIKNALLEN
                     EYIKKAFGVLLNDNDRKYICNSTQLELTNKTFIEAISSSSPMRGKKYNNNRPDLIILD
                     DYQSEENVRTEDAREKKFKKFSDDVKYAA"
     gene            complement(8136..8693)
                     /locus_tag="PC700_002478"
     CDS             complement(8136..8693)
                     /locus_tag="PC700_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF2581778.1"
                     /translation="MVVGYMRVSTAEQNEDRQLVTMKKHNVEKMYQEKISAKDTKRSK
                     LNAMLDFVREGDTIIVHDFSRLARSTKDLLEIVELLEKKHVKLISDKENLDTSTPTGK
                     LMLTMLGAIYEFERTNMLERQREGISIAKEQGKYKGRKEVKIDNFGSYYDRYKTRELN
                     KTQLAKELGVSRPTLDRLIKSHESN"
     gene            complement(8888..9076)
                     /locus_tag="PC700_002479"
     CDS             complement(8888..9076)
                     /locus_tag="PC700_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581779.1"
                     /translation="MNNKKKSFLISGTINVILLIIVFALFSYNIIENSTRNMIITVIL
                     IFIFEIIKIKILEKYYNL"
     gene            complement(9087..9662)
                     /locus_tag="PC700_002480"
     CDS             complement(9087..9662)
                     /locus_tag="PC700_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581780.1"
                     /translation="MLKKKIISFLMAGIFVLSSGYNLVYASTFEENEYKQELVNENEF
                     TIINSNENEMIVDKKGNNTYVSTKVTPGSWKNVKYLGQKSRKLSSPYTRERIIQTIQW
                     GLGYLSGGSSFLLGLAESIYREKVYDITKYGGVYTKTYYRRQITDIRNSHANVPVPYR
                     YEYVTYFYSSSNFKKVVYTERVGDGIKLANN"
BASE COUNT         3544 a         1333 c         1254 g         3804 t
ORIGIN      
        1 taaattttta ttcaaagaat acacataaat atttccagac tcacttattg tataagttat
       61 attctatttt gtatgaagtc aattaaaaac tgaactatat taaaattaat tttataaata
      121 acaaatataa ataattacaa aaccttaact gcgttaagtt tgaaatataa taatattaaa
      181 aacacttctc tcaaatcaag tatcttttgt tatattttca taagttttaa aaatataaat
      241 tttttaaaac ttgtgtccaa aaaacaaaat taaatatgtt atatatgtaa agggttaatt
      301 ttaaaacctt aaatttatta tagttagggg gatttaaatg aatatcaaaa gaattaatct
      361 ccttttatca acagtgatgg cagctgtgat aatatcttta ccacttgctg ttacagcaaa
      421 tgcaatggaa aaaacagaat cagataataa tgtgaaaata gagttgtctg acaaagaagt
      481 agaggaatta cataattact ggcctgagga taaaattttt tcagatgaaa agattgatga
      541 gatcttaatt aaaacaggtc aatttactca aaaggaattg gataaaatca atcaagaagt
      601 ggacgattct gctaagaaag taaatcaaaa ttctcgtgtg aaatatataa atgggtataa
      661 caaatacaga acttttacta aaaatggtaa aaactattta tatatttatg taagtggaaa
      721 gacattaaag aaaataaaag ctggaacaga tcttgcatct acttttggag gttttttacc
      781 aaaaatttgg gtagagcttg atgtagtggc aattggaaag ataataacaa ttaacatgga
      841 tggtataaat actaattatg ggattgtttt atcatatata gaggataagt ggaattttca
      901 gggtgcaaca tatacttata gatatgatca ttggttctat caaacttaaa agacatgctt
      961 ttttaggagg aataatatga aaaaaaggct ttataaggtt tttttaaaaa caattccaaa
     1021 cctgataatt gtgatattat cacttgcatt atcattttat actaatacat ttgatattga
     1081 tttaaaaata ttgcttttaa tacctttagc atatgtactt ttgtttatat actattacat
     1141 caaaacaaaa aaagattaat ttttatggaa ttagatatat acgaaaataa ttaatttaaa
     1201 aatttaactt tgagaatcat ttgtagagtt aaggccctta taatcccgaa aatggatttg
     1261 aagggccttt ttactagata tctttaaaaa gcttgttaag catagttata ttctatttaa
     1321 gtagagttaa ttatatattt atgttataat tgaactttaa aatattaatt cagaattttt
     1381 atattggagg aattctaaat gttaatttat ctagctatac ttgaactcga agaagataaa
     1441 ataaagtttg aaaaggtata tgaaaagtat agacaaataa tgttttatgt agccaataaa
     1501 atattaaagg atgatcattt atcagaagat gcagtacata atgctttttt aagaataatt
     1561 aaaaatatag ataaaattga tgaggtagac agtccaagaa cgaaggcttt tatcgttata
     1621 atagtagaaa ggattgctat agacttttac agaaagagaa aaagagaaaa ggtatctgat
     1681 attgaagaag agtataaaaa cagggagata aatttttcta tagaagataa agtatgtgaa
     1741 agtaatttag ctatagcact tgcaaaactt aatgaaagtt actttcaagt gctttcctta
     1801 aagttccagt atggtttttc aaataaagaa atagcaaatg cattaaattt aggggaggaa
     1861 aatgtatata agagaattca gcgtgctaga aaaaaattaa aagaaatatt agaagaaatg
     1921 gaggttggaa atattgagta ataaaaaaga tgattttgat attacggatg atatgcttag
     1981 agaaagtatc ccaatagcga caaaaattat tattgatgac cttctttcag atgatgaatt
     2041 aaatcatgtt ttttcaaaag gttttgagcg aaaaatgaaa aaactcatta agcaacaaaa
     2101 aagaagtagt cttacaaata aaatcatttt ttattcaaaa caaacagcta ttgtattttt
     2161 aatagtttta gctagtttac ttactattac tatgagtagt gaggcattgc gttcacgttt
     2221 ctttagatta gtaataagag tatatgaaga cttaacttca tttgtttttt caactaatgg
     2281 agaagataat cttagtttaa aaaataaaga acctgagtat ataccaaaag gatttaaaga
     2341 agttgataga gttgatgatt ttgttattac atatagaaat gatgaaaata ttgagataca
     2401 atatgtaaca gataaaatta gttcaaattc tattatatta gatacagaaa atgcaaaagt
     2461 taaagatata tttgtaaatg ggcataaagc aaaatatata gtaaaaggaa ctttcttaca
     2521 gttattttgg aatgacaaca actttatata tttattaaat gtagattatc aaaatagtgg
     2581 agaatctgaa ttttatgaag atattttgat aaaaatagcg aaaagtataa aataattttt
     2641 ttaagtgtcc aagggtagcc tcttcattcg ttatacaagt atatatgaaa atgtgaggtg
     2701 atttttatgg ttttaaaatc tacatttacc tgagcgttaa aaatattgtt ataggaaaaa
     2761 ttaatacatt atagttataa catgcttgca ataggaataa aactttaaaa ttgaggtgta
     2821 aaaaatgaaa tcagatttat atttatgtaa aagtaataaa ttgtatagag acaacgaagt
     2881 tctagaaaaa tttatggaag atttaagttt tcttatagac gattataaat tctcgttaga
     2941 aacaatatct attctatgca aaataaatat tgagaaatta actaatttct atgcaggaaa
     3001 aggatatata ctgtatgaag aaatgtctct tattgaaagt gtacttgcac cttttttaag
     3061 tgcaattgat attgctaaac ataattatga tgttgtcatt aaatcgaggg aaagcgataa
     3121 acctttgata ttcgaaaatt aatttaaaac tgggggaata cacatgaaaa gattatttgc
     3181 gttattactt actttaagtt tagttatatt accatcaact ttagtatttg ctaatgaaaa
     3241 agctgaaaat gattcaattt atgctgaggc gccattagtg tttcaaagta atgaaaaagc
     3301 atatacacat ggtagtggag gaactgcata tataggttat atgtcaagta aacatcttaa
     3361 ttggaggata acatcttcaa aaggaatagt tatactattt tcaggaactg ttgagattag
     3421 aaattcaaaa aatatgcttg tagcatcata tccaataagt ggtggaggat cttcaactat
     3481 atctggacaa atagatgtta aatatcttaa aaaaggggat tataaagcta tattgaaagg
     3541 tacaagtaca acaggaggcg gagtcttagt agtaaaatct ggtctagttc agatgtttac
     3601 aatttaatat tataagtaaa ataaaaatca tgaatttatt gggggatagt cacaatacat
     3661 attatttaca gatattcata actattatag ttaaagacat tctattataa agaaaatgta
     3721 taatttaaaa ttataataaa aaaaattatc attagtgtcc aaaaatcact tcttcattcg
     3781 ttatataagt tatataaata gaaaacaaat gaagaggtga ttttatgaat cttagaaaaa
     3841 cagttataag tggtatgtgt gctttagtat gttcatcagc agttgcaata cctgcatttg
     3901 ctgatgaata tcaagataag aatattgaac aatattcagc agtaaactat atagatgata
     3961 ctatggtgaa aatgtcttat attaagtctg gtacttgcaa tttaagtatt tcaggaagta
     4021 cagctaaagt aacatgcttt gttagaggag catcaaatgt tacaagcaca tcaattattg
     4081 ctagattaca gaaaagcaat gataatggga aaacatggtc aacaatccaa acatggaatg
     4141 cttcaggaaa tatttcatgt tctctttcta agtcaaaaac tataggaaaa ggatattaca
     4201 gagttcattc cacaataaaa gctaactctg aaagtaaaaa tataataagt agtacaaaga
     4261 aatattaaaa atagatattg tatttatgag ttgtagtaaa caagaataat agttttttaa
     4321 aagcaaatat aattaatttt aaatagagtg ccaatataaa ataggtgtat aatatttata
     4381 cttttttata ttggcactct attttataaa ataagttatt atagaaaatg aaacataatt
     4441 tatatataaa ttttctgttt gacctggtat ataatctttt atatcacata tcagtatttt
     4501 cccatcatta taaccccaac caatcacagt tgcaggtatc atatcagctt ctccatcata
     4561 aatgattgta tgtttataca tgatttttcc ctcgctatta tctatagttt tattcaaaat
     4621 accttctgct attaatttag caactatgtc tttaatagtc tgtatctgct ttactgtcta
     4681 caaaacatac ttcaattaat atcgctggag cttttgtatg actaagccaa tacaattccc
     4741 ttacgtccga ttttgcacct ctattttcag cagatttata tatatcttca gtcaatttag
     4801 ttataatctt ttgattcatc taataaaatc tctccctctg aaactatttc ctgttctttt
     4861 ttagcctttt ccatttctgc atgaggatta tcaataaaac ttggttgaga taatccggtt
     4921 tcagtagata atttatctcc aagttgactt aatatatttg ctacaactaa atcattatgt
     4981 ggtatcttag gggtaaattt agccttaata tctttataat catagtcaaa accatatttt
     5041 ttattaagcc aaatgcacaa taatttgtac ctattaaata aagtatctgc aatactatca
     5101 ttattctggc tacaaatcca ttctgtactg attaatcttg ctacaactgc taaacttgat
     5161 gtattacttt gcattttttc attgtgattt atatgagatg taattttata tatgttttct
     5221 tttattgaag atagtgtatt ttgaacaaat gtatcattca tgtctttaat aagccattct
     5281 atttttccat ctccattaac ttgaagtatt ccaagttctt tcatccttgg caaatcatct
     5341 tctttaatat tacattcaga gaaggttaaa taagcatttc taaaatcact aatttcatta
     5401 cttatatcac ttaaattagt ttcataagca tcttgtaatc cttttatatc attgaatatt
     5461 gtattttttc ctagttcttc acttctttta caaattgata ttggaacatt accattaaat
     5521 atattattta ctgtaggttt ttctatctct ttaaaatttg aatcaaaatg atatatgtat
     5581 tctttatcat atacatctat ataaatatca tctttaaaat cttttttaaa ttctctaaaa
     5641 aaatagatgg tctctccatc ttcattttca taatggcagc catctgttgg ttttattatt
     5701 cttactttca tttcattgtt ttttacatag taaagttcat agcaaaatcc aaataatagt
     5761 gtatatctca ataaatctga atcgtgcttt ttactccaat ggcaagtata gtattctaaa
     5821 tcatttataa tcttctcatc acctaatcta cttgtatagg tgattttatt tgcaagagaa
     5881 taagcgacct cttcattaat aaactttttt aagaaattag tgtttatctt attattagac
     5941 ctcttggtaa ccattttata gttactaata gcatctgttt cacctttata atacttatac
     6001 atagtgttgt attcttgctt ttttaaactc tagtcttcat atatttctct taataaactt
     6061 aaatctattt ccatttaatg catcacctcc atacaatcaa agacctaaat ttcttctatc
     6121 taatagtgta atactttcat ttactttaat attaaaaatc ctatttataa attctgctga
     6181 tatatctggt gcatcatcat gtagagagta cttttgtcct gtaaaattaa ggatttgctg
     6241 tataaatgcc ttatcctctt ctgcaaaaat catttgacct ttatttaaaa caggtattaa
     6301 agtagagatt ttatcatctt tatttttctt ttggtgctca ttaatgattt ctatatctct
     6361 gtagtaaaga acatcatcat tttttatttt aagttctaat tgattagcag ctgtaccatt
     6421 aaatgtattt ttttctatat aaacatgtgt tatatctgga tacactttta ataaataaat
     6481 catatgcaat atatatttat caaaatcagt tctagcattt attttggcta gttctgccaa
     6541 tctgccacat agtaaaccat tttctgattc acttccaaca agaaaagcac tatagtcatg
     6601 tttagaacca cctgcagatg ctgggtcaca taataacatt gtctttttga atgtatgagt
     6661 ttctatacat tctctagatt aagttctgat tgttttaaac catttctcac ctattgaatt
     6721 tacatcattt tgtacctctt gtttaaaaga tgttgggttt tcgtaataac ttaaagccat
     6781 gtctaaacaa ttccaaaaac ttggccataa tataggaaat ttaatttctt tttcattttc
     6841 ccagtaaaat tctttagcat cttcaagatg atttgtgttt ttgaaatcaa ataacaactc
     6901 tttgaaatta agccataaac cagaattaaa taattcatct acattatcaa ctaaaactcc
     6961 tttttcacat ttaaatttcc aagttggtaa attctttagc ctactataga aacattcttt
     7021 atgttgtaag gttcctaatg ctataaaagt tgttcctctt tttataatct taccatttct
     7081 tattactggt ttttaagcag catattttac atcatcagaa aattttttaa attttttctc
     7141 tctagcatct tctgttctaa cattttcttc tgactgataa tcatcaagta tgataagatc
     7201 tggacgatta ttattgtatt tttttcctct cattggtgat gaagatgata ttgcttcaat
     7261 aaaggtttta ttagttaatt ctaattgagt actattgcaa atatattttc tatcattatc
     7321 atttaagaga actccaaatg cttttttaat atactcattt tcaagtaatg cattttttat
     7381 atcttttacg aatttttctg ctgtagagcc tatatcagag caaattaaag tatatgtctt
     7441 atgtttataa caatgtgacc atacagttgt tgctaaatct ccaaatgcac ttttacctgt
     7501 tcctcttgga taaattcttc ctaattgctg tgaaccatct ccaattatag actcttgtat
     7561 atcttcccat aactcatgat gaactttagc tataggagca gcaacattat cttcttttgg
     7621 taaatatgtg tcttgtataa aatacataca gaaaaattct aaatttattt ttccaagctg
     7681 ataagctagg ccattaactc cccaaagatt gcttgaatta gtttttataa tctcatttgc
     7741 ttctttttca gcttctctta tagttgctcc atttcttata aaggtctttg ccatataact
     7801 aaatattaat tgtatatttc tttctaaatt atccactcgt atttacttcc tcttaattct
     7861 tctatttttt taggttcagt gagtaataca attaataaaa taaacaatgc aaattttttt
     7921 ctaataaaat caccctcttt gaaatatgtc tctggttttt aatttttgct agaaaatttc
     7981 tagaactcat tttctataaa taatactttt ttgaaataga aggatacccc ctatactgaa
     8041 aaacagttat ttataattat tttaaacata attaaagggc ataattctat gaatttgccc
     8101 cactaaaatt aacttaagca tatatattgc tcttcttagt tactctcatg gctctttatt
     8161 aatctatcta atgttggtct acttactccc aattctttag ccaattgagt cttattaagt
     8221 tctctagttt tatatctatc ataataacta ccaaagttgt ctatcttaac ttctttacgt
     8281 cctttatatt taccctgttc tttagctata gagatacctt ctcgttgtct ttctaacatg
     8341 tttgttctct caaattcata tattgcgcca agcattgtaa gcataagttt tcctgtaggt
     8401 gtacttgtat ctaaattttc tttatcacta attaacttta catgtttctt ttctaagagc
     8461 tcaactattt ctaataagtc tttagtactt ctagcaagtc tactaaagtc atgtacaatt
     8521 attgtatctc cttctcttac aaaatctaac attgcgttta gttttgatct tttagtatct
     8581 ttggcactta ttttctcctg atacattttt tctacattat gctttttcat tgtaactaat
     8641 tgtctatctt cattttgttc tgctgttgaa actctcatat aacctactac catctttgat
     8701 acctccacct atgtttactc tataaataat attatagcat aaatgtaaaa ctagagttaa
     8761 agatttgcca tgctttttgt aaattaattg gagttttgat tttattttta catcagaata
     8821 gatagtttgt aatgtaaatt tgtaccagca tactttacaa tatgctctta ggaaagatat
     8881 ttcttgttta tagattataa tatttctcta gtatctttat ttttataatt tcaaaaataa
     8941 aaattaaaat tacagttatt atcatatttc gtgtactatt ttctataata ttataactaa
     9001 acaatgcaaa aacaatgatt aataaaatta cgtttatagt gccagaaatc aagaatgatt
     9061 ttttcttatt attcatatat ttcctcctag ttatttgcta gttttatacc atctcctact
     9121 ctttcagtat atacaacttt tttaaagtta gatgagctat aaaaatatgt aacatattca
     9181 tatctgtatg gaacaggaac attagcatga ctgtttctaa tatcagttat ttgtctccta
     9241 tagtatgttt ttgtatatac gcctccatat ttagtaatgt catatacttt ttctctataa
     9301 atactctcag ctaaacctaa taaaaaagat gaccctccag aaagataacc taatccccat
     9361 tgaatagttt gtataattct ttctcttgta taaggagaac ttaatttgcg tgatttttga
     9421 cctagatatt taacattttt ccaactacca ggtgtaactt ttgttgaaac ataagtgtta
     9481 ttgccttttt tatccacaat catttcattt tcattgctat taataatagt aaattcgttt
     9541 tcattaacaa gttcttgttt atattcattt tcttcaaaag tagatgcata aaccaaatta
     9601 tatccactag ataaaacaaa tattcctgcc attaaaaaac ttattatttt tttctttaac
     9661 ataatatttt actaccttta cttaaatatt tattctataa acactgtcat ataacctgtt
     9721 agttatatta aaaataatct ttttatataa cttcacaatc aaaccttgtc ttatttcata
     9781 atagttttat ttaatatcat ataatttaga cctccttata agattattta tatatataac
     9841 gaataataaa agtaattttg gacaaaataa aaatcttttt attttaagaa tgaaaaattt
     9901 aaccctctaa gaaaactcaa ttaagagcga ttcat
//
LOCUS       DAMJWP010000127         9884 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.132, whole genome shotgun sequence.
ACCESSION   DAMJWP010000127 DAMJWP010000000
VERSION     DAMJWP010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9884
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.132"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            152..1396
                     /locus_tag="PC700_002481"
     CDS             152..1396
                     /locus_tag="PC700_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDF2581781.1"
                     /translation="MFENLKKADPEIYESMKRELKRQQRNIELIASENIVSVPVMETM
                     GSHLTNKYAEGYSGKRYYGGCEYIDEVETLAIDRIKKIFGAEHANVQPHSGANANIGV
                     YFAMLKPGDVVMGMNLSQGGHLTHGAPVNISGQYYKFYEYGIDKDSGLIDFDEVRKIA
                     HEVKPKMIVAGASAYPREIDFKKFREIADEVGALLMVDMAHIAGLVAAGIHQNPCEVA
                     DFVTTTTHKTLRGPRGGVILCKEKYAKDIDKAIFPGIQGGPLEHIIASKAVCFKEALS
                     DEFKEYQVQVAKNAKALAEELIKRDFKLISGGTDNHLILLDLINKNITGKAAEKRLDD
                     AYITANKNTIPFDPNGALVTSGIRLGTPAVTTRGMKEEDMAIIAEAIDLCLTYDEESK
                     ARTLVVGLTEKYPLYEEYNIMD"
     gene            1719..2816
                     /locus_tag="PC700_002482"
     CDS             1719..2816
                     /locus_tag="PC700_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-diphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDF2581782.1"
                     /translation="MKVLILTGKFGMGHYSASNSLSEDIKAKFDNSEITIKDIFEYIM
                     PNYSDKMYKTFSILVNRGSSLYNLFYKCAENGKKDIKFTFSDYFLNKLDTLLHEVQPT
                     VVISTFPFCSQLVSRYKEKYNSNLPLITCITDISSHSEWISKNTDCYLVASKSTKEEL
                     VFKGIDESKIKVNGIPVKKEFKRIEHVNHSTKKNILIMGGGLGLLPKSEQFYKELNSL
                     EGVKTTVITGNNKKMYYKLYGRYENIEVVGYTNEVYKYMKDSDLIISKPGGITLFETI
                     YSELPILAFNPFLQQEIDNASFILNNEIGRILGKNKKYYVDEIKDLIYDDATLKEMSS
                     NMKELKKQFDNNTLENILFSLDEQGACRECM"
     gene            2807..3505
                     /locus_tag="PC700_002483"
     CDS             2807..3505
                     /locus_tag="PC700_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2581783.1"
                     /translation="MYVVGLIIVVALIFLVHSIIPTYYNKLLNKEVLKNMAGENEIAL
                     TFDDGPDKRYTEKLLDVLKENDIQAMFFVVAKNAEKEPEIIKRMLRENHIVGLHSLEH
                     RNAWLYSYSYVKKDFIESTNIMKNLGVDVNYYRPPWGHTNIFSNSFVKKYNLKMTLWD
                     VMAEDWEKDSTVDIIINKLMSRTKENSIICLHDAGENSGGAVGAPERTIEALKIAIPK
                     LKASGLKFVTPERM"
     gene            3508..4572
                     /locus_tag="PC700_002484"
     CDS             3508..4572
                     /locus_tag="PC700_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDF2581784.1"
                     /translation="MISKTEKKNKCLGSVAFLVLLMGITGYFVFRGQSVESLIKSLKG
                     ASPMFILIGFAMMFIYVACEGINIYLGMKALNQKTTLLKCMGYAFIGFYFSSITPSAS
                     GGQPAQVYYMKKDDINISYSSLILLVIVVIHQVVILAYSGIMFIMEREFILNNVSGMN
                     ILLIYGVITNVALVIGVIAIIFSKKLVNNFIISITNLLGKLRIIKDVESSRKVIISQI
                     EEYVKGAQYIKQNPKLVVQILVITIVQITAMFLVPFFVYKAFHLSTYTVFEILAIQSL
                     LNIAVSSLPLPGAVGASENSFMTLFKIFFPGHLLVPAMLLSRGISFYAFVAISGLICI
                     VVHVKSSEKVKAVKKVAYVR"
     gene            4755..5459
                     /locus_tag="PC700_002485"
     CDS             4755..5459
                     /locus_tag="PC700_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2581785.1"
                     /translation="MNKEIPKKILVADDNPEIREIVEILLTGEGYEVIMATNGEEAVN
                     LVDSTIDLIILDVVMPVKTGFVACNEIREKTTAPILFLTAKTQDSDKVIGFSAGGDDY
                     LSKPFSYSELISRVKSLLRRYYIYQGKEKEREPNILKTKELCVNLDTQDVTLNGEPVF
                     LTTIEYSILILMLKNRKKVFSSENLYETIWDEQYFYTANNTIMVHIRNLRKKLEKDQK
                     NPQYIKTAWGKGYYID"
     gene            5452..6774
                     /locus_tag="PC700_002486"
     CDS             5452..6774
                     /locus_tag="PC700_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2581786.1"
                     /translation="MINKIKPSKLGVILLIYVVISAAVAFILNIPFYSAVESIVKDVS
                     EGKVYFKREVNQLMDNFQTYVNNNGIRADDGDDIAKWNSDNWFVSMKIYKGNKVFYDT
                     LYYPNEVPDKSEENEFYSSPMARNYEIQFSDIKANIVVVALFRFRFQNFIEAISITFM
                     ALTFIITFLLLFSRKIKYLRKIEMGIKIVESGSLKYRIPVKGNDELSSLASSINEMSH
                     TLENQLIKEREMKNKNKDIVTSISHDVRTPLTSVICYLDLIKDHKYNSPEQLEQYIKN
                     VRSKAYQIKDLTDDLFTHFVNSGEEVSKGYEKINANEFIFQLLLDSSYTLEEKGFHIT
                     IENELYNQYHIYVDVTQFRRIFDNLCSNIIKYARKDKPIVFLIKVEDSKLLIIQKNRV
                     SNFSKNVESFGIGIKNCEKIVAYHNGSINVNSDDKEFAIEISIPAKES"
     gene            6961..8334
                     /locus_tag="PC700_002487"
     CDS             6961..8334
                     /locus_tag="PC700_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581787.1"
                     /translation="MVLTGAHYIYILFMVIILITMIMKKDTIVPCILGVFFMGLFFEK
                     NIFGAITAVFNSFIISLNELGPIILIIAIMVALSKALEANNAIQYMVRPFSRVIKNSN
                     TAFFVTGFVMLVLSWFFWPTPAVALVGAVFLPVAMRAGLPAIGVAVALNLFGHGLALS
                     TDFVIQGAPSITAGAAGVAVSYVINDGMILFWVMGIVTISMAFYTLKRDINKGMFREE
                     LKGFESEEVEEFNGKSKIATILVALGFLADIIVMYIFDLKGGDASALLGGTAVFLIII
                     INVINFGKDSLENVCENIIDGFVFGIKIFGAIIPIAAFFYMGEVAPLTGVFGKVLAPG
                     SQGLLSDIGIALSQTIPLNKFAVSGIETVVGAITGLDGSGFSGISLVGSLASVFGTAI
                     NASVGALAALGQISGIWVGGGCLVPWGLISAAAICGVSPIELAKRNFVPVITGLVVTT
                     IVAMFII"
     gene            complement(8707..9645)
                     /gene="pdaA"
                     /locus_tag="PC700_002488"
     CDS             complement(8707..9645)
                     /gene="pdaA"
                     /locus_tag="PC700_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HDF2581788.1"
                     /translation="MFRKVLHYLTLLSISIFFIVGCSNSQNNQNENQNKETQLQEDKE
                     KIDGGKDTSNVIVSDGTDKPSKATTNNDNNKLDVSSLDNTTLDWFYIPNNKHKTPEVN
                     TDIEFKFSDYDALYNGPTKDGQKTLYLTFDEGYENGYTTKILDTLKQNQVKAVFFVTA
                     PYIKENKDLVKRMVSEGHIVGNHSKTHPSMPTKTSNLKNFNDELYDVEKLYKDVTGKD
                     MVKFFRPPMGKYSEKSLAMTKNLGYKTVFWSFAYRDWDTDKQPSHEEATQKIMDNLHD
                     GSILLLHAVSKTSTEILNDFISNARKLGYEFELLEY"
BASE COUNT         3664 a         1101 c         1685 g         3434 t
ORIGIN      
        1 aagaaagtaa ctatgttaaa tatggaaaaa tggcaaaaca cttgactatt aattggtaaa
       61 ggactataat aataaattat catattgata aaaacgaaaa ttatgaaatg tctgtaaaaa
      121 taaaatttaa ataataacta ggaggagtaa tatgtttgag aacttgaaaa aagcggatcc
      181 tgagatttat gaaagtatga aaagagagtt aaaaagacaa caaagaaata ttgaattaat
      241 agcatctgaa aacatagtat cagttccagt aatggaaact atgggaagtc atcttacaaa
      301 taagtatgca gaaggttact caggcaaaag atattatggt ggatgtgaat acatagatga
      361 agttgaaact cttgcaatag atagaatcaa aaaaatattt ggagcagaac atgctaatgt
      421 tcaacctcac tctggagcaa atgcaaatat tggtgtatat tttgctatgt taaaaccagg
      481 tgatgttgtt atgggaatga atttatctca aggtggacat ttaactcatg gtgctccagt
      541 taatatttct ggtcaatatt ataaatttta tgaatatggt atagataaag acagtggact
      601 tatagatttt gatgaagtta gaaagatagc acatgaagta aaacctaaaa tgatagttgc
      661 aggagcaagt gcatatccta gagaaataga ttttaaaaag tttagagaga tagctgatga
      721 ggttggagca ttgcttatgg tagatatggc tcatatagca ggtcttgtgg ctgctggtat
      781 tcaccaaaat ccatgtgaag tggctgattt tgttacaaca acaactcaca aaactttaag
      841 aggacctcgt gggggtgtta ttctttgtaa agaaaaatat gctaaagaca tagacaaagc
      901 aatattccct ggtattcaag gtggacctct tgaacatata attgcttcta aggcagtttg
      961 ctttaaagaa gctttaagtg atgagtttaa agaatatcag gtacaagttg ctaaaaatgc
     1021 aaaagcctta gctgaagaac ttattaaaag agattttaaa ttgatttctg gaggtacaga
     1081 taatcattta atactattag atttgattaa taaaaatata actggtaaag cagcagaaaa
     1141 gagattggat gatgcttata taacagctaa taaaaataca attccatttg atccaaatgg
     1201 tgctcttgtt acaagtggaa taagacttgg tactccagct gtaactacta gaggtatgaa
     1261 agaagaagat atggcaataa tcgctgaggc tatagaccta tgtttaactt atgatgaaga
     1321 atctaaggct agaacattgg ttgttggttt aactgaaaaa tatcctttat atgaagaata
     1381 taatattatg gattaattat aaagaacaac taaataaaat acatttaaat aattacaatg
     1441 attgtgtatg tatttgaata tatttagata taaaaaaggt gtctcaaaat gaacttttta
     1501 gtttatgagg cacttttttg tatggaatcg aacatatttg tgttatttaa ataataaaaa
     1561 atatggttat ctctattttt agatggtctc tttgtatttt ttaattaaaa ctttaagatt
     1621 tcttaagaat tatactaggt ttttctaaag atttaaagtc tatacttata agtatagtaa
     1681 tcatatgaaa agaaaagctt taacgaggag ggaatcttat gaaggtatta attcttacag
     1741 gaaaatttgg tatgggtcat tactcagcat caaattcttt aagtgaagat ataaaagcaa
     1801 aatttgataa ttcagaaata acaattaaag atatatttga atacattatg cctaactatt
     1861 cagataaaat gtacaagact ttttctattc ttgtaaatcg gggaagtagt ctttataatt
     1921 tgttttataa atgtgctgaa aatggaaaga aagatataaa atttactttt tcagattatt
     1981 ttttaaataa attagataca ttacttcatg aagtacaacc aacagtagtt atttcaactt
     2041 ttccattttg ttctcaatta gtatcaagat ataaggaaaa atataactca aatcttcctt
     2101 taataacatg cataactgat ataagtagtc actcagaatg gattagtaaa aatacagact
     2161 gttatttagt agctagtaaa tctactaagg aagaacttgt ttttaaagga atagatgaat
     2221 ctaagattaa agttaatggt attccagtaa aaaaagaatt taaaagaata gaacatgtaa
     2281 atcattcaac taaaaaaaat attcttataa tgggtggagg attaggatta ctgcctaaat
     2341 cagaacaatt ttacaaagaa ttaaatagtt tagaaggagt aaaaactact gtaataactg
     2401 gtaataataa aaaaatgtac tataaactat atggtagata tgaaaatata gaggtagtag
     2461 gttacaccaa tgaagtatat aaatatatga aagattctga ccttattatt tctaaaccag
     2521 gtggaataac actatttgaa acaatttatt cagaacttcc aatattggca tttaatccat
     2581 ttttacaaca agagatagat aatgctagtt ttatattaaa taatgaaatt gggagaatac
     2641 ttggtaaaaa taaaaaatac tatgtagatg agataaaaga tttaatttat gatgatgcta
     2701 ctcttaaaga aatgagcagt aacatgaaag aattaaaaaa acaatttgac aataatacct
     2761 tagagaatat tttattttca cttgatgaac aaggagcgtg tagagaatgt atgtagtagg
     2821 tttaattatc gttgttgcat tgattttttt agtccattca attataccta catactataa
     2881 taaactatta aataaagagg tattaaaaaa tatggctgga gaaaatgaaa ttgcacttac
     2941 atttgatgat ggtccagata agagatatac agaaaaatta ctagatgtgt taaaagaaaa
     3001 tgatatacaa gctatgtttt ttgtagtagc aaagaatgct gaaaaagagc ctgaaattat
     3061 aaaaagaatg ttgagagaaa atcatatagt tggtttacat tcattggaac acagaaatgc
     3121 atggctttat agttatagtt atgttaaaaa agattttatt gaaagtacaa atataatgaa
     3181 aaatttaggt gtagatgtaa actactatcg tccaccatgg ggtcatacta atatattttc
     3241 aaattcattt gttaaaaaat ataatctaaa aatgactttg tgggatgtaa tggcagaaga
     3301 ttgggagaaa gatagtacag tagacattat tataaacaag ttaatgagta gaacaaaaga
     3361 aaattctata atttgtttac atgatgcagg tgaaaattca ggtggtgcag taggtgcacc
     3421 tgagcgtact attgaagctt taaagatagc tattccaaaa cttaaagcca gtggtttaaa
     3481 gttcgtaaca ccagaaagga tgtagttatg ataagtaaaa ctgaaaagaa aaataaatgc
     3541 ctaggtagtg tagctttttt agttttacta atgggcataa caggatattt tgtttttaga
     3601 gggcagtcag ttgaatcatt gataaaatca ttaaaaggtg caagccctat gtttatacta
     3661 attggatttg caatgatgtt tatatatgtt gcctgtgaag gtataaatat atacttaggt
     3721 atgaaagctt taaaccaaaa aactactctt ttaaaatgta tgggatatgc ttttattgga
     3781 ttttatttta gttctattac gccttcagca tcaggtggtc aaccagcaca agtttattat
     3841 atgaaaaaag acgatataaa tatatcatat tcttcactta tactattagt cattgttgtt
     3901 attcaccaag tagttatact ggcatatagt ggaataatgt tcattatgga acgtgagttt
     3961 atattaaata atgtaagtgg tatgaacatt ttactaatat atggtgtaat aacaaatgtt
     4021 gctcttgtca ttggagtaat tgcaattata ttttcaaaaa aacttgtaaa caattttata
     4081 atatcaataa caaatttatt aggtaagtta agaataataa aagatgttga aagttcaaga
     4141 aaagttataa tatctcaaat tgaagagtat gttaaaggtg ctcaatatat aaagcagaat
     4201 ccaaaacttg tagtccaaat tttagtcata actattgtac aaataacagc tatgttttta
     4261 gttcctttct ttgtatacaa agcttttcat ctatcaacat atactgtatt tgagatatta
     4321 gcgatacaat ccttgcttaa tattgctgtt tcttcattac cattgccagg ggctgttgga
     4381 gcttctgaaa atagttttat gacattattt aagatatttt tccctggtca cttattagtt
     4441 ccggcaatgt tacttagtag gggaataagt ttttatgcat ttgtagctat aagtggattg
     4501 atatgtatag tagtacatgt aaaatcttct gaaaaagtaa aagcagtgaa aaaagttgct
     4561 tatgtaagat aatattatat agattatttt tattaatatg tcaatttgtg tgtacatttt
     4621 aattaagttt taaacatgtt tacaggagta taaactatta aaacgtgtga cattgctaag
     4681 cttttgctaa taaagtataa tattttatat agtttgaaat atttaagaaa tatagcaaaa
     4741 aaaggagctt ggtgatgaat aaagagattc caaaaaaaat tttagtagct gatgataatc
     4801 ctgagattag ggaaatcgta gaaatacttt taactggtga aggttatgaa gttattatgg
     4861 caacgaatgg agaagaagct gtaaacttag tagatagtac gattgatttg attattttgg
     4921 atgttgtaat gcctgtaaaa acaggatttg ttgcatgtaa tgaaattaga gaaaaaacga
     4981 cagcaccaat attattttta actgcaaaaa ctcaagactc tgataaagtt attggatttt
     5041 cagcaggagg agatgactat ttatcaaagc ctttttcata ttctgaacta atttcaaggg
     5101 ttaagtcgct acttagacgt tattatatat accagggaaa ggaaaaggaa agagagccaa
     5161 atatattaaa aacaaaagag ttatgtgtca atttagatac tcaagatgtt acactaaatg
     5221 gagaacctgt gtttttgaca actatagaat atagtatatt aattcttatg ttgaaaaata
     5281 gaaaaaaagt tttttcgtct gaaaatttat atgaaactat ttgggatgag cagtattttt
     5341 atacggcaaa taatacaata atggttcata ttagaaattt aagaaaaaag ttagaaaaag
     5401 accaaaaaaa tccacaatat ataaaaaccg catggggaaa ggggtattat attgattaac
     5461 aaaattaagc catctaagtt gggagttata ctattgatat atgtagtaat ctcagctgca
     5521 gtggctttca ttttaaatat accattttac tctgctgttg aatctatagt aaaagatgta
     5581 tcagaaggga aagtatattt taaaagagaa gtaaatcaac ttatggataa ttttcaaact
     5641 tatgtgaata acaatggtat aagagcagat gatggagatg atatagcaaa atggaatagt
     5701 gataattggt ttgtttctat gaagatatat aaaggaaata aggtttttta tgatacatta
     5761 tactatccta atgaagtacc tgataagagt gaggaaaatg agttttatag cagtcccatg
     5821 gctagaaact atgagataca attttctgat attaaagcta atatagttgt agtagcttta
     5881 tttagattta gatttcaaaa ttttatagaa gcaatttcta ttacatttat ggcacttaca
     5941 tttattataa catttttact gttgtttagt agaaagataa agtacctaag gaaaatagaa
     6001 atgggtataa agatagtaga gagtggcagt ttaaagtata gaatcccagt aaaaggtaat
     6061 gatgaattat catcacttgc atctagtatt aatgagatga gtcatacatt ggagaatcaa
     6121 cttataaaag aaagggagat gaagaataaa aataaagata tagtaacttc gatatcacat
     6181 gatgttagaa ctccattaac atcagtaatt tgttatttag atttgatcaa agaccataaa
     6241 tacaatagtc ctgagcaatt ggaacaatac ataaaaaatg taaggtctaa agcatatcaa
     6301 ataaaagatt taacagatga cctttttacg cattttgtta attctggaga ggaagtttca
     6361 aaagggtatg aaaaaataaa tgctaatgaa tttatttttc aattactttt agatagttca
     6421 tatacattgg aggagaaagg atttcatata acaatagaaa atgaacttta taaccaatat
     6481 catatttatg tagatgttac acaatttaga aggatatttg ataatttatg ttctaatata
     6541 ataaaatatg ctaggaaaga taagcctata gtatttttaa ttaaagtaga ggattctaaa
     6601 ttactaatta tacaaaaaaa tagagtaagt aatttttcta agaatgtaga aagttttgga
     6661 attggtataa agaattgtga gaaaatagtt gcatatcata atggaagcat aaatgtaaat
     6721 tcagatgata aagaatttgc tattgaaata tctattccag caaaagaatc ataaaaattt
     6781 aaattatata ttgtgtaatt gctattatat attgtgtaat tgcttatgta aaattctatg
     6841 aagcaggaag catgtgactt tagttatggg atgttcaata ttgtgtacct ctaggcaagt
     6901 aacaagttta cttacaagac catataatat tattatctta aaaaaagggg acataataat
     6961 atggttttga caggagcaca ttatatttac attctattta tggtaattat attaattact
     7021 atgattatga aaaaggatac aatagtacca tgtatattag gtgttttttt tatgggattg
     7081 ttctttgaaa aaaacatctt tggtgctata acagcagtgt ttaactcttt tattatatct
     7141 ttaaatgagt tgggtccaat tatcttgatt atagctatta tggttgcatt atctaaagcc
     7201 ctagaagcca ataatgctat acagtatatg gtgagaccat tttcgagagt tataaaaaat
     7261 tctaatacag cattttttgt aactggattt gttatgttag ttctttcttg gtttttctgg
     7321 ccaactccag ctgttgcctt agttggagct gtatttttac ctgttgctat gagagctggg
     7381 ctaccagcaa ttggtgttgc agtagcatta aatttatttg gtcatggtct tgccttgtct
     7441 acagactttg ttattcaagg agctccaagt ataacagctg gtgcagcagg tgttgcagta
     7501 tcatatgtta taaatgatgg tatgattttg ttttgggtga tgggaatcgt aaccatatct
     7561 atggcatttt atacattaaa aagagatatt aacaaaggaa tgtttagaga agaactaaaa
     7621 ggttttgaaa gtgaagaagt agaagagttc aatggaaaat ctaaaatagc aactatttta
     7681 gtagctctgg gatttcttgc tgatataatt gttatgtata tatttgacct aaaaggagga
     7741 gatgcatcag ctcttttggg tggaacagca gtatttttaa taataattat aaatgttata
     7801 aattttggca aagatagtct tgaaaatgta tgtgagaata taattgatgg ttttgtgttt
     7861 gggataaaaa tatttggagc aattatacca atagcagcat ttttctatat gggtgaagtt
     7921 gcgcccttga caggtgtatt tggaaaagta ttagcaccag gttcacaagg tttattatca
     7981 gatataggca tagctctttc tcaaacaata ccacttaata aatttgccgt atcaggtata
     8041 gaaactgtag tgggggcaat aactgggctt gatggttctg gattttctgg gatttcatta
     8101 gttggttcac ttgcctctgt atttggaact gctataaatg caagtgttgg tgcattagct
     8161 gcattaggac aaatcagtgg tatatgggta ggtggaggct gtctagttcc ttggggtctt
     8221 atttcagcag cggcaatttg tggggttagt ccaatagagt tggcaaagcg aaactttgtc
     8281 ccagttataa ccggattggt tgtaacaacc atagtagcaa tgtttattat ttaataaatc
     8341 gaataaatct ttaattgtac aaattaaaaa tagatgtgta gctataattt attataattg
     8401 ttttactaat tttaagccat acatctattt ttattgatat ttttattagt attctttaat
     8461 aaaataatat ttttcattat actatacata acatctaaat ataacatcta aaatactgtt
     8521 atctatgttt aatatcttgc caaatttttt gatgttttat taccctgtcc caccaacata
     8581 tataagctat cgcaagtttt ttacctatac tatcaccaaa caaaaatttt aattttaatt
     8641 tattatttta taatctatat tatattaact atattagttt gtagacttta gatgtaattc
     8701 taaatattaa tactccaaga gttcaaattc atatcctagt tttcttgcat ttgatataaa
     8761 atcatttaaa atttctgtac tagttttgga aactgcatgt agtaacaaga tagaaccatc
     8821 atgtagatta tccattattt tttgcgttgc ttcttcatgt gatggttgtt tatcagtatc
     8881 ccagtctctg tatgcaaaac tccaaaatac agtcttgtat cctaaatttt tagtcattgc
     8941 caagcttttt tctgagtatt ttcccatagg aggtctaaaa aatttaacca tatctttccc
     9001 agttacatct ttatataact tttcaacatc atataattcg tcattaaagt tttttagatt
     9061 gcttgttttt gttggcatag atggatgggt tttactatga tttccaacta tatgtccctc
     9121 agaaaccatt ctttttacta aatctttatt ttcttttata tatggagctg taacaaaaaa
     9181 aactgcttta acctggtttt gttttagagt gtcgagtatc tttgtagtgt atccattttc
     9241 ataaccttca tcaaatgtaa gatatagtgt tttttgacca tcttttgtag gtccattgta
     9301 tagggcatca tagtcagaaa atttaaattc aatgtccgta tttacttctg gtgttttatg
     9361 cttattgtta ggtatataaa accaatcaag agtagtgtta tctaaagaag atacatctaa
     9421 tttattatta tcattattag tagtggcttt tgatggttta tctgttccgt ctgaaactat
     9481 tacattggaa gtatccttac caccatctat tttttcctta tcttcttgta actgtgtttc
     9541 cttattttga ttttcgtttt gattgttttg tgaatttgaa catccaacaa taaaaaaaat
     9601 agaaatagaa agtagtgtta aataatgcaa tacttttcta aacattttga agctcctttc
     9661 taaattaata ataattgcat atagtattat tataattgga aattactatt aataatagtt
     9721 atatctttaa aaataaagtc acaatagtat gacaaagggt gaataatcaa taaatttaaa
     9781 aaaaaatgaa aaaaatagtt gaccatatta tgagaagatg ttattataat aaatgtgctc
     9841 aacaagaagt aacaaaataa caagcacaaa gaagttaatt gaat
//
LOCUS       DAMJWP010000128         9787 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.133, whole genome shotgun sequence.
ACCESSION   DAMJWP010000128 DAMJWP010000000
VERSION     DAMJWP010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9787
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.133"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            72..1943
                     /locus_tag="PC700_002489"
     CDS             72..1943
                     /locus_tag="PC700_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp2"
                     /protein_id="HDF2581789.1"
                     /translation="MNKKNLSVIMAAAMISTSVAPVFAAETTQVKKETITKKEATELV
                     SKVRDLMSQKYTGGSQVGQPIYEIKVGETLSKLKIITNIDELEKLVNALGENKELIVT
                     ITDKGHITNSANEVVAEATEKYENSADLSAEANSITEKAKTETNGIYKVADVKASYDS
                     AKDKLVITLRDKTDTVTSKTIEIGIGDEKIDLTANPVDSTGTNLDPSTEGFRVNKIVK
                     LGVAGAKNIDDVQLAEITIKNSDLNTVSPQDLYDGYRLTVKGNMVANGTSKSISDISS
                     KDSETGKYKFTIKYTDASGKAIELTVESTNEKDLKDAKAALEGNSKVKLIAGDDRYAT
                     AVAIAKQTKYTDNIVIVNSNKLVDGLAATPLAQSKKAPILLASDNEIPKVTLDYIKDI
                     IKKSPSAKIYIVGGESAVSNTAKKQLESVTKNVERLAGDDRHMTSVAVAKAMGSFKDA
                     FVVGAKGEADAMSIAAKAAELKAPIIVNGWNDLSADAIKLMDGKEIGIVGGSNNVSSQ
                     IENQLADVDKDRKVQRVEGETRHDTNAKVIETYYGKLDKLYIAKDGYGNNGMLVDALA
                     AGPLAAGKGPILLAKADITDSQRNALSKKLNLGAEVTQIGNGVELTVIQKIAKILGW"
     gene            2050..2757
                     /locus_tag="PC700_002490"
     CDS             2050..2757
                     /locus_tag="PC700_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDF2581790.1"
                     /translation="MVDATLFFTPHQDDETLSMGSAIIEHVEKSDTHVILCTDGSKSI
                     IRKVLDDGGSCSYHIKDVHKYSLSESEFSKDRDEEFKDSCEAMGVKESNIHIEDNRAH
                     DGELSKEKAREIILKYLEEYPDAKVKTVTPFKASGIHEDHRALGEAALELYKEGKIKD
                     LRFYVEPYDYKDFKKVNPNVEVWKVLPSQEEKLLSAMNAYKKWNPESGHYAIGYHSVK
                     SHFDELAINKIQYVHAP"
     gene            2790..4625
                     /locus_tag="PC700_002491"
     CDS             2790..4625
                     /locus_tag="PC700_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF2581791.1"
                     /translation="MKISKKIVSLLTMTFLTVTLYGNTSNASTKDTLTGSGRWETAIK
                     ISQAGWKKSENAVLVNDNSIADALSATPFAKAKDAPILLTQSNKLDSRTKAELKRLGV
                     KNVYLIGGSIALSSEIEKQLNAENISFERISGNSRYDTSLKLAEKLDREKSISKIVVV
                     NGEKGLADAVSVGAIAAQENMPIILSDSENGTEVADNFIDSKDIEKSYVIGGTYSISN
                     SVERSLPNATRIAGSSRSETNAKIIEEFYKDTDIKNIYVTKDGTRSKHDLIDSLAVGV
                     LASKNGSPILLAGNKLDSSQKDVLNTKIIDKVIQIGGLGNENVVEDVIDMQEETKYTV
                     ETIDELNVAIKKSDANDIIRFKPEKDKNITNSFKIETNKAITIEFDGIYKQSITIDMP
                     NGDIKNFGDIDNDLVINNIKNETLVNEGSIQGIDVYDENGCKIENEDSGEIWLITILK
                     NSKDVYILNRGEITKISNNSSSVTIKNSGNINTISGSEEPSISGKKPITNDTDKDEDK
                     KARGLYPKVESCIPALKDYVRITISTKPESSKYKIYYRVVDEKPSAKYVGEKISTKGW
                     SMVTISNQSFIERAKDGSYIEAVEIDTSNNEVSRWGKSSITDDGI"
     gene            complement(4673..5068)
                     /locus_tag="PC700_002492"
     CDS             complement(4673..5068)
                     /locus_tag="PC700_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HDF2581792.1"
                     /translation="MILKKHRETILYLFFGAFTTLVNIVSYLFFTRVILFNFMVANAL
                     AWILAVLFAYVTNKFFVFESKRIEIRFVFKEFLSFVSFRLLSGVVEMLIMYVMIDLLF
                     VNDVIVKIFTNIVVIVLNYLFSKMIIFKK"
     gene            5295..7706
                     /locus_tag="PC700_002493"
     CDS             5295..7706
                     /locus_tag="PC700_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp84"
                     /protein_id="HDF2581793.1"
                     /translation="MRKYKSKKLSKLLALLTVCFLIVSTIPVSAENHKTLDGVETAEY
                     SESYLQYLEDVKNGDTAKYNGVIPFPHEMEGTTLRNKGRSSLPSAYKSSVAYNPMDLG
                     LTTPAKNQGSLNTCWSFSGMSTLEAYLKLKGYGTYDLSEEHLRWWATGGKYGWNLDDM
                     SGSSNVTAIGYLTAWAGPKLEKDIPYNLKSEAQGATKPSNMDTAPTQFNVTDVVRLNK
                     DKETVKNAIMQYGSVTSGYAHYSTYFNKDETAYNCTNKRAPLNHAVAIVGWDDNYSKD
                     NFASDVKPESNGAWLVKSSWGEFNSMKGFFWISYEDKTLLTDTDNYAMKSVSKPDSDK
                     KMYQLEYAGLSKIMSNKVTAANVFDFSRDSEKLDSVMFETDSVGAKYEVYYAPVVNGV
                     PQNNSMTKLASGTVSYSGYINVPTNSYSLPKGKGAIVVVIDNTANPNREKSTLAYETD
                     IDGYYLYEAKANLGESYILQNNKFEDINTYSEFSPCNFVIKAITKTSSGQATSGESLT
                     GADRYETAVKVSQKGWTSSQNAVLVNGDAIVDALTATPFTAAIDSPILLTGKDNLDSK
                     TKAELQRLGTKKVYLIGGENSLSKNVQTQLSNMGISVERISGSDRYKTSISLAQKLNS
                     IKSVSQVAVANGVNGLADAISIGAAAADNNMPIILTNEKSELQGADEFLNSSKITKSY
                     IIGGTATLSSNLESKLSNPTRLAGSNRNETNAKIIDKFYPSSDLKYAFVVKDGSKSQG
                     DLIDGLAVGALGAKTDSPVVLVGNKLDESQKNVLKSKKIETPIRVGGNGNESAFNELN
                     TLLGK"
     gene            8003..9580
                     /locus_tag="PC700_002494"
     CDS             8003..9580
                     /locus_tag="PC700_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp5"
                     /protein_id="HDF2581794.1"
                     /translation="MKAPKTILTILTIALTLSSISIIPSYALTEEKLIGNGRYETAVK
                     ISQKAYSSSSNVVLVNDNSLADALSATPFAKAKGAPILLTESDKLDDRTEKEIKRLGA
                     KDIYLIGGTAVLNKDIENKLKGNGLNVERINGKDRYETSLILANKLKDIKDIKEVAVV
                     NGEKGLSDAVSVGAPAAQNKMPIILSSPKDGVEAFDKFIRDEKVIKAYVIGGTNSVSR
                     AVEKSLPNAERVSGKDRNETNAKVIEKFYTDTNLSNLYVTKDGSKNENQLIDSLAVGV
                     LAAKNESPVVLVGNKLNTKQRDILSTKKLNTITQVGGNGNEEAFDEIKSLQEKTVFEA
                     KTVEELTDMINIASPNDIINFKPKENTVNEAFRMVTNKPITVNIKGDCSKTLTVDMPN
                     GEVNNYATLVNVIVRNIGEGGFNNHDTITILSVRDKNGRVIENTRNSDIDTLMILASA
                     NDTKLINDGYIGKLIDNSSNSDITNNGTIDKKVNQVENLEAKVDSIEKAIDSISQKVN
                     KIQDILDKIGFLKKFLS"
BASE COUNT         4069 a         1135 c         1756 g         2827 t
ORIGIN      
        1 tcaaatatat attataattg gtaaataagg aaaaaataat aaaaatttga attttttagg
       61 gggaaaatac catgaataaa aaaaatcttt ctgtaattat ggctgctgca atgataagta
      121 catcagtagc tccagttttt gctgcagaaa ctacacaggt aaaaaaagaa acaataacta
      181 agaaagaagc tacagaacta gtttcgaaag ttagagattt aatgtctcaa aagtatactg
      241 gtggttctca agttggacaa ccaatatatg aaataaaagt tggcgagact ttatcaaaat
      301 taaaaataat aactaatata gatgaattag agaaattagt aaatgctttg ggagaaaata
      361 aagaacttat tgtaactata acagataaag ggcatataac aaatagtgca aatgaagtag
      421 ttgcagaagc aactgaaaaa tatgaaaatt cagcagacct ttccgctgag gctaattcta
      481 taacagaaaa agctaaaact gaaactaatg gaatttataa agttgcagat gtaaaagctt
      541 catatgatag tgctaaagat aagttagtta taactttaag agataaaaca gacacagtaa
      601 cttctaaaac tatagagata ggtattggtg atgaaaaaat tgatttaaca gcaaatccag
      661 ttgattcaac gggaacaaac ttagaccctt ctacagaagg atttagagta aataaaatcg
      721 ttaaactagg tgtagcagga gctaaaaata ttgatgatgt ccaattagct gaaataacta
      781 taaaaaatag tgacctaaat acagtttcac cacaagattt atatgatgga tatagattaa
      841 ctgttaaagg taatatggta gcaaatggaa catcaaagtc aattagtgat atttcatcaa
      901 aagattcaga aacaggaaag tataaattta ctattaagta tactgatgca tctggaaaag
      961 caatagagct tactgtagaa agtactaatg aaaaagattt aaaagatgcc aaagctgcat
     1021 tagaaggtaa ttcaaaggtt aaattgatag ctggagatga tagatatgca actgcagtgg
     1081 ctatagcaaa acaaacaaaa tatactgaca atatagttat agttaattca aataaactag
     1141 ttgatggatt agcagctaca ccacttgctc aatctaaaaa agcacctata ttattagcat
     1201 ccgataatga aataccaaaa gtaactttag attatataaa agatataatt aagaaaagcc
     1261 catcagctaa aatatatata gtaggtggag aatcagcagt atcaaataca gctaaaaagc
     1321 aattagaatc agtaactaag aatgttgaaa gactagctgg agatgataga catatgactt
     1381 ctgtagcagt agcaaaagct atggggtctt ttaaagatgc atttgtagta ggtgcgaaag
     1441 gggaggctga tgctatgagt atagctgcca aagctgctga acttaaggct cctataatag
     1501 taaatggctg gaatgatctt tcagcagacg ctatcaaatt gatggatgga aaagagattg
     1561 gtatagttgg tggttctaac aatgtatcta gtcaaattga aaatcaactt gctgatgttg
     1621 ataaagatag aaaagttcaa agagttgaag gagaaacaag acacgatact aatgctaagg
     1681 ttatagaaac atattatgga aaattagata aactatatat agcaaaagat ggatatggaa
     1741 ataatggtat gctagtagat gcattggcag caggacctct agcagcaggt aaaggtccaa
     1801 tacttctagc taaagctgat ataacagact cacaaaggaa tgcacttagt aaaaaattaa
     1861 atcttggtgc agaagtaact caaataggta atggagttga attgacagta atacaaaaga
     1921 tagctaaaat actaggttgg taataactta tactgtggag aagattcctt aattaataca
     1981 ggaatctttt tctataatta tgctatttat tgtaaattat aacattttat aaagtaggag
     2041 gatgtagtag tggtagatgc aactttattt tttacaccac atcaagatga tgaaacctta
     2101 agtatgggaa gtgctataat tgaacatgtt gaaaaaagtg atacacatgt catattatgc
     2161 acagatggta gtaaatctat aataagaaaa gttttggatg atggagggtc atgttcttat
     2221 catattaaag atgttcataa atactcttta tcagaaagtg aattttcaaa agatagagat
     2281 gaagaattta aggatagctg tgaagcaatg ggtgtaaaag aaagtaatat acatatagaa
     2341 gataatagag cacatgatgg tgagttaagt aaagaaaagg caagggaaat aatacttaag
     2401 tacttagaag aatatccaga tgcaaaagta aaaactgtta ctccgtttaa agctagtgga
     2461 atacatgaag accatagggc attaggagaa gctgcactag agttatataa agaaggcaaa
     2521 ataaaagatt taagatttta tgtagaacca tacgactata aggattttaa aaaagttaat
     2581 ccaaatgtag aggtatggaa ggtattacct agtcaagagg agaagttatt aagtgcaatg
     2641 aatgcatata aaaaatggaa tcctgaaagt ggacattatg ctataggata tcattcagta
     2701 aaatctcatt ttgatgagct agctataaat aaaatacagt atgtacatgc tccataaaat
     2761 attatgaaag aattaggagg taagaattaa tgaaaatatc aaaaaagata gtgtctttgt
     2821 taactatgac atttttaact gttacattat atggaaatac atctaatgca tctacaaaag
     2881 atacattaac aggttctgga agatgggaaa cagcaataaa aataagtcaa gcaggatgga
     2941 aaaagtctga aaacgctgtg ctagtaaatg ataattcaat agcagatgct ttatcagcta
     3001 ctccatttgc taaagcaaaa gatgcaccaa tattattaac tcaaagtaat aaattggata
     3061 gtagaacaaa agcagaatta aaaagacttg gtgtgaaaaa tgtatattta ataggtggtt
     3121 caattgcatt aagttcagag attgaaaagc agttaaatgc agaaaatata agttttgaaa
     3181 gaatatctgg aaatagtaga tatgatactt ctttaaaatt agctgaaaag ctagataggg
     3241 aaaaatctat atctaaaata gtagtagtaa atggagaaaa gggtcttgct gatgcagtga
     3301 gtgttggagc tatagctgct caagaaaaca tgccaataat actttctgat tcagagaatg
     3361 gaactgaagt agctgataat tttatagata gtaaagatat agaaaaatcg tatgtaatag
     3421 gtggtacata ttctatttct aattctgtag aaagaagttt accaaatgca acaagaatag
     3481 caggtagtag tagaagtgag acaaatgcaa aaattataga agaattttac aaagatactg
     3541 atataaaaaa tatttatgta acaaaggatg gtacaaggag taagcatgat ttaatagatt
     3601 ctttagctgt gggtgtatta gcttctaaaa atggttctcc aatactttta gcaggaaata
     3661 agctagatag ttctcaaaaa gatgtgttaa acactaaaat tatagataaa gttattcaaa
     3721 ttggcggatt gggtaatgaa aatgttgtag aagatgtaat agatatgcaa gaagaaacta
     3781 agtatactgt tgaaactatt gatgaactta atgttgctat aaaaaaatca gatgcaaatg
     3841 acatcataag atttaagcca gaaaaagata aaaatataac taattctttt aaaatagaaa
     3901 caaataaagc tataactatt gaatttgatg gtatatataa acaatcaata actatagata
     3961 tgccaaatgg agatataaaa aactttgggg acatagataa tgatctagta ataaataata
     4021 taaaaaatga gactttagtt aacgaaggaa gtatacaagg gatagatgtt tatgatgaga
     4081 atggatgtaa aatagagaat gaggattctg gagaaatatg gcttataacg atacttaaaa
     4141 attcaaaaga tgtatatatt ttgaatcgtg gagaaataac taaaatatct aataattctt
     4201 ctagtgtaac aataaaaaat tcaggtaata ttaatactat aagtggaagt gaagaaccat
     4261 caataagtgg taaaaaacct ataactaatg atacagataa agatgaagat aaaaaggcac
     4321 gtggtttata tcctaaagta gaatcttgta tacctgcttt aaaagattat gtaagaataa
     4381 ctatatcaac aaaaccagag agttcaaagt ataaaattta ttatagagta gttgatgaaa
     4441 aaccatctgc gaaatatgtt ggagaaaaaa ttagtactaa aggttggagt atggttacaa
     4501 tatcaaatca atcttttatt gaaagagcca aagatggatc atatatagaa gcagtagaaa
     4561 tagatacctc aaataatgaa gtttcacgat ggggaaaatc tagcattaca gatgatggaa
     4621 tttagacatt aaacttattc attttgcaga ttactattta gtaatctgct atttattttt
     4681 taaaaattat catcttgcta aacaagtaat ttaaaactat aacaactata ttagtaaata
     4741 tttttacaat aacatcattt acaaaaagca aatcaatcat tacatacata attaacattt
     4801 caacgacacc agaaagtagc ctaaaactaa caaaagacaa aaattcttta aatacgaacc
     4861 ttatttcaat tcttttactt tcaaatacaa aaaacttatt agtaacataa gcaaataaaa
     4921 cagcaagtat ccatgccaaa gcatttgcaa ccataaaatt aaataaaata actctagtaa
     4981 aaaataaata cgaaactata tttacaagag tagtaaatgc tccaaaaaat aaatataata
     5041 ttgtctctct atgctttttt aaaatcaaat ataatcctcc taatctaatt taatataaat
     5101 atacttaaag atactaacaa acattagtat agcacatcta ttaataaatc aatatatctt
     5161 aaaatattat aaaaataaaa ctataatatt ctataattaa tatgtactca taatccatat
     5221 tttatcaaaa taaaatatga ccttaattta gaaatctgat ataataaaaa ttaaatacat
     5281 ataagggggt aaacatgaga aagtacaaat caaaaaaatt gtcaaagcta ctggcattgt
     5341 tgacagtttg ctttttaata gtgtcaacaa tacctgtctc agcagaaaac cataaaactc
     5401 tagatggagt agaaactgca gagtattctg aaagttatct tcaataccta gaagatgtca
     5461 aaaatggaga cacagcaaaa tataatggag taataccttt tccacatgaa atggaaggta
     5521 caacacttcg aaataagggc agaagtagtc ttccatcagc atataaatca agtgtagcat
     5581 acaacccaat ggatcttggt cttactacac cagcaaaaaa tcaaggaagt cttaatacat
     5641 gctggtcttt ttcaggtatg tcaactttag aagcatatct taaattaaag ggatatggaa
     5701 cgtatgactt atcagaagaa catttaagat ggtgggcaac tggtggaaaa tatggatgga
     5761 acttagatga tatgtcaggt agttcaaatg taacagccat aggatatctg acagcatggg
     5821 caggtcctaa gttagaaaaa gatataccat ataatcttaa atctgaggca caaggtgcaa
     5881 ctaaaccttc aaatatggat actgcaccta ctcaatttaa tgtaacagat gttgttcgtc
     5941 ttaataaaga taaggaaact gtaaaaaatg ctataatgca atatggttca gtgacatcag
     6001 gttatgcaca ttattcgaca tattttaata aagatgaaac agcatataac tgtacaaata
     6061 agagggctcc attaaatcac gctgtagcga tagtaggatg ggatgataat tattcaaaag
     6121 ataactttgc atctgatgta aaaccagaat caaatggagc atggttagta aaaagcagtt
     6181 ggggagaatt taattctatg aaaggattct tctggatttc ttatgaagat aaaactctac
     6241 taacagatac agataactat gcaatgaaat cagtatcgaa accagatagt gataaaaaaa
     6301 tgtaccaact tgaatatgct ggtcttagta agataatgtc aaataaagta acagcagcaa
     6361 atgtatttga ttttagcaga gattctgaaa aacttgactc tgttatgttt gaaacagact
     6421 ctgtaggagc aaaatatgaa gtatattatg caccagtagt aaatggagtt cctcaaaaca
     6481 actcaatgac aaaacttgca agtggaacag tatcatattc tggatacata aatgtaccta
     6541 ctaattctta cagcttacca aaaggtaaag gagcaatagt agtagttata gacaacacag
     6601 caaatcctaa tagagaaaaa tcaactttag catatgaaac tgatatagat ggatactatc
     6661 tatatgaggc taaagcaaac ttaggtgaaa gctacatact tcaaaacaat aagtttgaag
     6721 atataaatac atatagtgaa ttttctcctt gtaactttgt tataaaagct ataacaaaaa
     6781 catcttctgg acaagctact tcaggagaat ctttaactgg agcagataga tatgaaacgg
     6841 cagttaaagt tagtcaaaaa ggatggactt cttcacaaaa tgcagtatta gtaaatggag
     6901 acgcaatagt ggatgcttta acagctacac catttacagc agcaatcgac tctccaattc
     6961 ttttaacagg aaaagataac ttagattcaa aaactaaggc agagttacaa agattaggaa
     7021 ctaaaaaagt ttatctaata ggtggagaaa attctttaag caagaatgta caaactcaac
     7081 ttagtaatat gggtatatca gtagaaagaa tttcaggtag tgatagatac aagactagta
     7141 tatctctagc tcaaaagtta aatagtataa aatctgtttc acaagttgca gtggcaaatg
     7201 gtgtaaatgg acttgcagat gcaataagta ttggtgcagc agctgctgat aacaatatgc
     7261 caataatact tactaatgaa aagagtgagt tgcaaggtgc tgatgagttt ttaaattcat
     7321 caaaaataac taagtcttat ataattggtg gtacagctac tttatcatca aatttagaaa
     7381 gtaagctttc aaatccaaca agacttgcag gaagtaatag aaatgaaact aatgctaaga
     7441 taatagataa attctaccct agctcagatt tgaaatatgc ttttgttgtt aaagatggtt
     7501 caaagagtca aggagacttg atagatggac tagcagtagg agcattaggt gctaaaactg
     7561 attcaccagt agttctagtt ggaaataagt tagatgaaag tcaaaaaaat gtacttaagt
     7621 ctaagaaaat agaaactcct attagagttg gtggaaatgg aaatgaaagt gcttttaatg
     7681 aactaaatac tcttttagga aaatagtgaa tggaaagata gtaaggatat acattttatt
     7741 attaaaaaat attcaataga agtttataaa tataaaataa caaaaagggg tgtctcaaaa
     7801 tgaacttttt agttcatgag gcactctttt ttgttgtttt atatttttat catttaaata
     7861 atgaaaaata ggcttgtcta tatttttaga tagttttctt attattgatg aaaaatgtaa
     7921 taattttaaa ctgtaaaata gtgtaaagtt attttataat ataaatatag taagatttta
     7981 aacaaaataa gggggaataa taatgaaagc accaaaaact attttaacaa tactgacaat
     8041 agcgcttact ttaagtagca tttctataat accatcatat gcacttacag aggaaaaatt
     8101 aataggtaat ggtagatatg aaactgctgt aaagataagt caaaaggcat acagttctag
     8161 ttctaatgta gtgttagtta atgacaattc attagctgat gcactttcag ctacaccatt
     8221 tgcaaaggct aaaggagcac caatactttt gacagaaagt gataagctag atgataggac
     8281 tgaaaaagag ataaaacgtc taggagctaa agatatttat ttgataggag gtactgctgt
     8341 acttaataaa gatatagaga acaagttaaa aggtaatgga cttaatgtag aaagaattaa
     8401 tggtaaggat agatatgaaa cttcactgat tttggctaat aagcttaagg atataaaaga
     8461 tattaaggaa gttgctgtag taaatggtga aaaaggatta agtgatgctg ttagtgttgg
     8521 agcaccagca gctcaaaata agatgcctat aattttgtct agtcctaagg acggagtaga
     8581 agcatttgat aaatttataa gagatgagaa agttataaaa gcatacgtaa ttggaggaac
     8641 aaattctgtt tcaagagctg tagaaaagag ccttcctaat gcagaaagag tgagtggaaa
     8701 agatagaaat gagactaatg ccaaagttat agaaaagttt tatactgata caaatttaag
     8761 taatttatat gtaactaagg atggtagtaa aaatgagaat caactaatag actcgttagc
     8821 tgttggagta ttggctgcaa aaaatgagtc tcctgttgtt ttggttggta ataaattaaa
     8881 cacgaaacaa agggatatac ttagtactaa aaaattaaat actataacac aggtaggtgg
     8941 aaatggaaat gaagaagctt ttgatgaaat taagagttta caagaaaaaa ctgtgtttga
     9001 agcaaaaact gttgaggaat taactgatat gataaatata gcaagtccta atgatattat
     9061 aaactttaaa ccaaaagaga atactgtaaa tgaagctttt agaatggtaa caaataaacc
     9121 aataactgtg aatataaagg gtgattgttc aaagactctt acagtagata tgcctaatgg
     9181 tgaagtgaat aactatgcta ctttggtaaa tgttatagtt agaaatattg gagaaggtgg
     9241 atttaataac catgatacaa tcactatatt gtcagttcgt gacaaaaatg gtagagttat
     9301 agaaaatact agaaattcag atatagatac gttgatgata ttagcttctg ctaatgatac
     9361 aaaattgatt aatgatggat atataggaaa gcttatagat aattcatcta atagtgatat
     9421 aactaataat ggtactatag ataaaaaagt taatcaagtt gagaatttag aggctaaagt
     9481 agattcaatt gaaaaagcta tagattctat aagtcaaaaa gtcaataaaa tacaagatat
     9541 actagataag attggatttc taaagaaatt tcttagttaa gaatatgtat atttaagtga
     9601 agtgtgtgtt gtaattgttc taaagttata tagttaaaca ggtttatata caaacagact
     9661 tatagataaa tatgtagttt agtatatatt tgaataagct ttggggaggt aacatgggga
     9721 ttggaattga atgtaacaaa aaaataaata aaagtattga caaaactaga ataaagaaaa
     9781 ataatct
//
LOCUS       DAMJWP010000129         9703 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.134, whole genome shotgun sequence.
ACCESSION   DAMJWP010000129 DAMJWP010000000
VERSION     DAMJWP010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9703
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.134"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(107..1138)
                     /locus_tag="PC700_002495"
     CDS             complement(107..1138)
                     /locus_tag="PC700_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HDF2581795.1"
                     /translation="MLKKKSSLTLKTIREILPGLFISVLVGYISIFISILIPKVGAAS
                     ISIFLGMFVGNLFLNQKVFQKGYKFSETDLLSYSIVLLGATLSVSTLIDLKVSGILFI
                     ILQMTITIIAALYIGKKLGFEENFRFLMASGNAVCGSSAIGAIVPVVNATDKEKGIAV
                     TIVNVTGIFLMFLLPIISQFLYSHELVKTSAMIGGTLQSIGQVVASGAIVGENVKDLA
                     TIFKIVRVIFLVVVVLVFGHMKNQSNREILEEEKDEIKKKKVTIPWYVIGFFITCALF
                     SMNIIPKEVSVLCKEISNKLEIIALAAIGLKVNVKDLVKQGKEVSLYGLFVGTVQVVS
                     AVVLIKIFI"
     gene            1326..2216
                     /locus_tag="PC700_002496"
     CDS             1326..2216
                     /locus_tag="PC700_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2581796.1"
                     /translation="MFEELKTFVAVVEYKNFTKAGEYLNLSQPSVSKHIKNLENYFKV
                     VLINRSIKQKTIFITESGQILYKRAKEILNLLNITYHDVSQVSDAITGHLKIGASLTI
                     GEYILPNFLALFSQKYPDIYVEVFIKNTSIVSSHVKDYILDIGLIEGTCSSPSFIQEY
                     FFEDKMVLALPYNSHLLKDFSFDKLQDQKWIVREDGSGTRDYLDMFLSVKEIIPKSMM
                     VFGSNYAVKESVRNNLGITIVSNLVTSLPVLNNELSVIELGSSYNRHFSYIFPKDITL
                     SKAATIFIEELKIFSNLNSI"
     gene            complement(2268..3194)
                     /locus_tag="PC700_002497"
     CDS             complement(2268..3194)
                     /locus_tag="PC700_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HDF2581797.1"
                     /translation="MKNQLNENHKKVVFFGVGAVGATFAEQFFNSKYDFKILCDNERK
                     KRYLEEGFIINGKRYDFNYVTKDEYKQEADFIIIGLKYNNLKENIKELDGLVGKNTVI
                     MSLLNGVDSEEIIGERFGIGKMVYSYVTNIDAKKINNNIIHTTNGIIVFGNKDNSEDR
                     KTNIITEVFDDVNIEYILSKDIQRDMWWKYMVNIGVNQTSAILGAPYGVFQSSEHLRE
                     LAKSAMREVVAIAQAKDISLTEDDVENSLHRILEHSKEGRTSMLQDVEAHRPTEVDMF
                     SKNICKLGKKYSIPTPINQTLFYMIKVIESRF"
     gene            complement(3409..3993)
                     /locus_tag="PC700_002498"
     CDS             complement(3409..3993)
                     /locus_tag="PC700_002498"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDF2581798.1"
                     /translation="MSKVDVVDILKKSDALLEGHFLLSSGKHSNRYVQCAKVLRFPEY
                     AAEVLSTVVEQIKDLDIDLVVGPAMGGVIVSYELGRQLGKEAVFTERKDNTMELRRGF
                     EVKKGAKIIIAEDVVTTGKSTMETKRVLEALGGEVVGVACIADRTNHDIGMPIYSAIK
                     LDIQVYESDECPLCKEGKLPVVKPGSREFKELGM"
     gene            complement(4078..4980)
                     /locus_tag="PC700_002499"
     CDS             complement(4078..4980)
                     /locus_tag="PC700_002499"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDF2581799.1"
                     /translation="MGNLSVKFGSVEFKNPVIMASGTFGFGKEYNEIYDIQKLGGISS
                     KGLTLNKKPGNNGMRVHETPSGMMNSVGLENPGVQGFIDYELPFFSKLDLVRIANVGG
                     GTLEDYLLGVQMLNDKPIDIIELNISCPNVKAGGMAFGIKNEVAREVVREVRNITKLP
                     LVIKLSPNAEDIVGMAKVCEEEGADGVSLVNTFKAMAIDIKNRRPVFENVYAGLSGPA
                     IKPIALRMVHEVCKNVNIPVMGMGGITKATDAIEFIMAGATCIQVGTANFMNPRIGIE
                     IIDGINEFMDREGIKSLDEIRGII"
     gene            complement(4973..5665)
                     /locus_tag="PC700_002500"
     CDS             complement(4973..5665)
                     /locus_tag="PC700_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HDF2581800.1"
                     /translation="MYKILENIYIGEDMYRMKVKGNFEGKMGQFYMLRAWDTYPVLSR
                     PISIHDIDEEGITFLYKVVGEGTQILSNLKVNDNIKLEGPYGNGYAKVDGKVALVGGG
                     IGVAPLYLVAKNIKNCDAYLGFREDVILEDEYKQVCNKVYTTVGNTFVTDIIDVEKYD
                     YILTCGPTPMMEKLVKMVEGTKTRIMVSLENHMACGVGACLVCTCKTNGGNKKTCKDG
                     PVFWGEDVIFNG"
     gene            complement(5704..6624)
                     /gene="pyrB"
                     /locus_tag="PC700_002501"
     CDS             complement(5704..6624)
                     /gene="pyrB"
                     /locus_tag="PC700_002501"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HDF2581801.1"
                     /translation="MLKSRNLIQPEDFSIEEIDEILELAQKIIDNPSKYSRICEGKLL
                     ATLFYEPSTRTRLSFESAMNRLGGRVVGFSEPNSSSASKGETLGDTMRIVSGYVDIIA
                     MRHPQSGAASEAARYTEVPFINAGDGKNQHPTQTLTDLLTIKSLKGTLESHTIGLCGD
                     LKYGRTVHSLVKAMARYKNTKFVFIAPEELKMPDYIKEAIKGHAYYETNNLDDVIGSL
                     DVLYMTRVQQERFEDKSEYERLKNYYILNKAKLEKASKDMLVMHPLPRVNEIDIDVDS
                     DDRAVYFKQAKYGMYVRMALIIKLLGINED"
     gene            complement(6920..7942)
                     /locus_tag="PC700_002502"
     CDS             complement(6920..7942)
                     /locus_tag="PC700_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF2581802.1"
                     /translation="MKKKILIPVFASVMALSVSSIVNADEVNDSSQNKDDKTNTELNL
                     GEYKEVKYKVAKIKDGVAIKIREEGQVQNIAYSGDEFTVLGTQGEWVKVKVEDGEGWL
                     ATRYVDISEGVGYTNADKVNLRKDKSESSEVIEELEKGSSLLVLEDNGDWLKVKDGET
                     EGYVKSSYISDKAPVIEEPQVNPSIDQNVDSTNNNQQNNANTNQQNNNSNVPTANSNA
                     VQAVLNLAYSKQGCPYVWGAEGPNTFDCSGFTQYVYRNAVGKNIPRTSKAQSKYGQTV
                     SKANLQPGDLVFFTTNGSGSVSHVGIYVGGGNMIHSPSTGKTVSVTSINSSYYTARFV
                     TAKRIL"
     gene            complement(8869..>9703)
                     /locus_tag="PC700_002503"
     CDS             complement(8869..>9703)
                     /locus_tag="PC700_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HDF2581803.1"
                     /translation="VYLIYTIFKYIKLKNLINDLSYDVDDREIVNLYKNILEEFNITK
                     DIKLKYSYEVETPAFFNSCVLLPPHEYKLKELDWIFRHELMHFKSRDLYLKYVILFLK
                     TVYWFNPFIYIMDKHIDLDCELYCDERVLKNRNSEEKKDYALTILNAMRKGSNTSNKF
                     IAGLHKQSDIKKRVMNMFNEKYKNGILMALTLCLLSSITFLKVNSTSIVNLNVLPKGF
                     EDLRTVSSIRFSVIGFTYADAPKNMREEYKINCESLGLIPKDSDKIEVGVEYYDSLLN
                     D"
BASE COUNT         3159 a         1701 c         1007 g         3836 t
ORIGIN      
        1 tatacccact taaacttatc gaaattcatt ttttatttat attataaatt attagtatca
       61 tattatttta attaatcaaa ttaatattac aaatactatt tttgtgctat ataaatattt
      121 ttattaaaac tacagctgat acaacttgta ctgtacctac aaataaacca tataaagaaa
      181 cttctttccc ttgtttcact aaatctttta cattaacttt taatccaatt gctgctaaag
      241 ctattatttc tagtttgtta cttatttctt tgcacaacac agatacttct tttggtataa
      301 tgttcattga aaaaagtgca caagttataa agaatccaat tacataccat ggtatagtta
      361 cttttttctt ttttatctca tccttttctt cttcaaggat ttccctatta gactgatttt
      421 tcatatgtcc aaatactaat acaacaacaa ctagaaatat tactcttact atcttaaaaa
      481 tagtagccaa atcttttaca ttttctccaa caattgctcc acttgctaca acctgaccaa
      541 ttgattgcaa agttccacct atcattgctg aagtttttac aagttcatgg ctatataaaa
      601 attgagatat tattggaagt aaaaacataa ggaatatacc agtaacattt acaatagtta
      661 ctgcaattcc cttttcttta tctgtcgcat taaccactgg aactattgct cctatggcag
      721 aagaaccaca tactgcattt ccacttgcca ttagaaatct aaagttttct tcaaatccta
      781 gttttttacc tatataaagt gcagctatta ttgttattgt catttgcaat attataaata
      841 aaattcctga aacttttaag tctattaagg tagatacact gagtgttgcc cctaatagta
      901 ctattgaata tgataacaaa tctgtctctg aaaatttata acctttttga aatacctttt
      961 gatttaaaaa taggtttcct acaaacatac ctaaaaatat agaaattgat gctgccccta
     1021 ccttaggaat taaaatagaa ataaatatac taatataacc tactaacact gatataaaca
     1081 gtcctggtaa tatttccctt atagttttta atgtcaaact gctttttttc tttaacataa
     1141 tatacctacc ttataattaa tatagttttc ttcatagcaa tagtatataa ttgtaataac
     1201 cataagtaaa ataaatatat aatatcgtaa tgattaatgt ttttaatggt tattatatat
     1261 agtataatta tctgtagaaa gtatataaaa aatgtaaaat ttcattaggt gaaggagaca
     1321 aaaaattgtt tgaagaatta aaaacatttg tagctgttgt tgaatacaaa aactttacta
     1381 aagctgggga atacttaaat ctatcacaac caagtgtaag taaacacata aaaaatctag
     1441 agaattattt taaagttgtg cttataaaca ggtctattaa acaaaaaacc atttttataa
     1501 cagaaagtgg acaaatattg tacaaaagag ctaaagaaat attaaatctt ttaaatatta
     1561 cttatcatga tgtaagccaa gtttcagatg ctataactgg tcacttaaag attggtgcaa
     1621 gtttaactat tggagaatat attctaccaa attttttagc attattttct caaaagtatc
     1681 ctgacatata tgtagaagtt tttataaaaa atacatcaat cgtatcttct catgttaagg
     1741 attatatttt agacattggt cttattgaag gcacttgctc ttccccttca tttattcaag
     1801 aatatttttt tgaagataag atggttttag cacttccata caatagtcat ttattaaaag
     1861 attttagttt tgataaactt caagaccaaa aatggatagt cagggaggat ggttctggaa
     1921 ccagagacta tttagatatg tttttaagtg ttaaagaaat tattccaaaa agtatgatgg
     1981 tttttggaag taattatgct gtcaaagaat ctgttagaaa taatttagga attacaatag
     2041 tttctaatct agtgacaagc ttacctgttt taaacaatga gctgtctgtc atagagcttg
     2101 gaagcagcta caacagacat ttctcatata tatttccaaa agatataact ttgtcaaaag
     2161 cagcaactat tttcattgaa gagttaaaaa tatttagtaa cttaaatagt atataacatt
     2221 aataaaattt aaaatcaata attatcaata tttcttacaa atacccttta aaatctactt
     2281 tcaattactt ttatcatata aaataaagtc tgatttatag gagtaggtat gctatatttt
     2341 tttccaagtt tgcagatatt cttagaaaac atatctactt ctgtaggtct atgagcttcc
     2401 acgtcttgaa gcattgatgt tcttccttct tttgaatgtt ctagtattct atgtaatgaa
     2461 ttttcaacat catcttctgt aagagatatg tcttttgctt gtgctatagc aacaacttcc
     2521 ctcattgcag attttgctaa ttctcttaaa tgctcagaac tctgaaaaac tccataaggt
     2581 gcaccaagta tagctgaagt ttgatttaca ccaatattaa ccatgtactt ccaccacata
     2641 tctcgttgaa tatcttttga taaaatatat tctatattta catcatcaaa gacttcagtt
     2701 attatattag tttttctatc ttcactatta tctttgttac caaatacaat tatcccatta
     2761 gtagtgtgta taatattatt attgattttc tttgcatcta tattggtaac atatgaatat
     2821 accatttttc caattccaaa tctttctcct attatctctt cgctatcaac tccatttagc
     2881 agagacatta taactgtatt ttttccaact aatccatcta attcttttat attttctttt
     2941 aaattattat attttagacc tataattata aaatcagcct cttgtttata ctcatcttta
     3001 gttacataat taaaatcata cctttttcca tttattataa atccttcttc taaatatctt
     3061 ttctttcttt cattatcaca aagaatttta aaatcatatt tagagttaaa aaattgttct
     3121 gcaaaagtag ctcctacagc acctactcca aaaaatacaa cctttttatg attttcattt
     3181 aattgatttt tcatacttac ccctccctaa tatataatac ttttacctta tacttatatt
     3241 gtactgctac tatctcttat tttgtaaata tatttttaac aaaaaaaaca gctaaccttc
     3301 atttataagg ctaactgctt tttattttta agttatatgt tatattacaa atttttactc
     3361 taaaaaattt acatttttta agtaacacta taaataatta agtttttatt acatccctaa
     3421 ttccttgaac tctctacttc caggcttaac aactggtaat tttccttcct tacataaagg
     3481 acactcatca gattcataaa cttgaatatc aagttttata gcactatata taggcatacc
     3541 tatatcatga ttagttctat ctgctataca tgcaacacct acaacttctc cacctaaggc
     3601 ttctaatact ctttttgtct ccatagttga tttaccagta gttacaacgt cttcagcaat
     3661 tattatcttt gctccttttt taacttcaaa tcctcttctt aactccattg tattgtcttt
     3721 tctctcagta aatacagctt cttttcctaa ttgtcttcct aactcataag aaactattac
     3781 tccacccata gctggtccta ctactaagtc tatatctaag tcttttattt gttcaacaac
     3841 tgtacttaat acctcagcag catattctgg aaatcttaat acttttgcac attgtacgta
     3901 tctattactg tgttttcctg aagataataa aaaatgtccc tccaacaatg catcactttt
     3961 ctttaatata tccactacat ctactttact catattattt ttcctccatg ttaatgttat
     4021 aaaattattg tttatttaat ctatattttt tatctttaaa atactattta ttctacctta
     4081 tataattcct cttatttcgt ctaaactttt tattccttct ctatccataa attcattaat
     4141 cccatctatt atttcaatac ctattctagg attcataaaa tttgcagttc caacttggat
     4201 acaagtagct cctgccatta taaactctat agcatctgtg gctttagtta ttccgcccat
     4261 tcccattaca ggtatattta catttttaca cacttcatgt accattctca aagctatagg
     4321 ttttatagct ggtccagata atccagcata tacattttca aatactggtc ttctattttt
     4381 tatatcaatt gccattgctt taaaagtatt aactaatgaa actccatcag caccttcttc
     4441 ttcacaaact tttgccattc ctacaatatc ttctgcattg ggtgataatt ttattactaa
     4501 aggcagtttc gttatgtttc ttacctcacg aacaacttct cttgccactt cattttttat
     4561 accaaaagcc attccaccag ctttgacatt tgggcaagat atatttaatt caataatgtc
     4621 tataggcttg tcatttaaca tttgaacccc taataagtag tcttctaatg tcccacctcc
     4681 aacattagct attctgacta agtctagttt actaaaaaaa ggtaattcat agtctataaa
     4741 tccttgaact ccagggtttt caagcccaac actattcatc ataccggatg gtgtctcatg
     4801 aactctcata ccattgttac caggtttttt atttaaagta agacccttgc tacttatacc
     4861 accaagtttt tgtatatcat aaatctcatt atattctttt ccaaatccaa aagttccaga
     4921 cgccattatt actggatttt taaattctac acttccaaat ttcacactta aattacccat
     4981 taaaaatcac gtcctctccc cagaacactg gaccatcttt acaagtcttt ttgtttcctc
     5041 cattagtttt acacgtacat actaaacagg cacctactcc acaagccata tggttttcaa
     5101 gagataccat aattctcgtt ttagtacctt ctaccatctt aactaatttt tccatcattg
     5161 gagttggacc acatgttaat atataatcat atttctcaac atcaattata tctgttacaa
     5221 aagtatttcc tactgttgta tagactttat tacatacttg cttatattca tcttctaata
     5281 taacatcttc tctaaaccca agatatgcat cacaattttt tatattttta gcaaccaaat
     5341 atagtggagc tacaccgatt cctcctccta ctagagctac ctttccatca accttagcat
     5401 atccattacc ataagggcct tctaatttta tattatcatt tacctttagg ttactcaaaa
     5461 tttgagttcc ttctcctact actttataaa gaaaagttat cccttcttca tctatatcat
     5521 gtatacttat aggtcttgaa agtactggat aagtatccca tgctcttaac atatagaatt
     5581 gtcccatttt accttcaaaa ttacctttta ctttcattct atacatatct tcgcctatat
     5641 atatattttc cagaatttta tacatcccaa aatctccttt gttttactat tatatgataa
     5701 ctattagtct tcatttatac ctaaaagttt tattattaaa gccattctaa catacattcc
     5761 atatttagct tgtttaaaat atacagctct atcatcacta tctacatcta tgtctatttc
     5821 atttactctt ggtaatggat gcattactaa catatcttta gatgcctttt ctaatttagc
     5881 tttatttaaa atataatagt ttttaagtct ttcatactct gatttatctt caaacctttc
     5941 ttgttgaact ctagtcatat ataaaacatc caagcttcct ataacatcat ccaaattatt
     6001 agtttcataa tatgcatgtc cctttatagc ttcttttata taatctggca tttttaactc
     6061 ttcaggcgct ataaatacaa actttgtatt tttatatctt gccatagcct taaccaaaga
     6121 atgcacagtt cttccatatt ttaaatcacc acataatcct attgtatggc tttctaaagt
     6181 tccttttaat gattttatag taagtaagtc tgtaagtgtt tgagttggat gctggttttt
     6241 gccgtctcct gcatttataa atggcacttc cgtatatcta gctgcttctg aagctgcacc
     6301 tgattgagga tgtctcatag ctattatatc aacataacca gatactatcc tcattgtatc
     6361 tcctagagtt tcaccttttg atgcagagga tgaatttggc tctgaaaagc ctactacacg
     6421 tcctcctaat ctgttcatag cagattcaaa gcttaatctt gttcttgttg atggttcata
     6481 aaataatgta gctaatagtt ttccttcaca tatacgagaa tattttgacg gattgtcaat
     6541 tattttttga gctaattcta atatttcatc tatttcttct attgaaaagt cttctggttg
     6601 gattaaattt cttgatttta acatctttcg tcctcctttt ttagcctctc tgaactaaaa
     6661 ttaaaagtat ttttgtactg ttagtagctt accaccttta ccctatcctg tcaagggatt
     6721 gcgcaaataa tcatcatatg aaaaggctcc tatttaatag tcatattaac ttgaatcttg
     6781 attagattta aagtgataca aaaacgctct atagtacatt taaaatataa tcattttttg
     6841 tttccatgat taaattaaat ataatataga gcgtttatat atatttatgt atttatcaat
     6901 ttgtcatatt acaaaccgat tataatattc tttttgctgt aacaaatctt gctgtatagt
     6961 atgatgaatt tatactagtt acactaactg tttttcctgt tgatggtgaa tgtatcatat
     7021 tacccccacc tacatatatt ccaacatgac taacagaacc agaaccatta gttgtgaaga
     7081 atactaagtc tcctggttgt aaatttgctt tacttacagt ttgtccatat ttagattgtg
     7141 ctttagatgt tcttggtata ttctttccta ctgcatttct atacacatat tgtgtaaatc
     7201 cagaacagtc aaaagtgttt ggtccttctg ctccccaaac atatggacaa ccttgcttag
     7261 aatatgccaa gttaagtaca gcttgaactg cattagagtt agctgttggc acatttgaat
     7321 tattattttg ttgattagta tttgcattgt tttgttgatt attatttgta gaatctacat
     7381 tttggtcaat acttgggttt acttgtggct cttctattac aggagcttta tcagatatat
     7441 aagatgattt tacatatcct tctgtttccc catctttaac ttttaaccaa tctccattat
     7501 cctccaaaac caataaagaa cttccttttt ccagctcttc aattacttct gaactttcac
     7561 tcttatcttt tcttaagttt actttatcag cgttagtata tcctacacct tcagatatat
     7621 ctacatatct agtagctaac caaccttcgc catcttcaac tttaacctta acccattctc
     7681 cttgagttcc taaaaccgta aattcatctc cagaataagc tatgttttga acttgacctt
     7741 cttctcttat ctttatagct actccatctt ttatttttgc aactttatat tttacttctt
     7801 tatattcacc taaatttaat tcagtatttg ttttatcatc cttattttga gatgaatcat
     7861 ttacttcatc tgcatttaca attgaactta ctgataatgc cataactgat gcaaatactg
     7921 gtattaatat ctttttcttc acgataaacc tcctaaaatt ttcccataat tatcttatga
     7981 ttctattttg taggtgaagt gtacctaagg ataaaatact aatacacaat acattaataa
     8041 aagctctatc tgtaactttt ttgtaaacca ctttatataa cttttatagt atcattatat
     8101 taataaaata aataaattag cttatccaat atgtcaccta aatatacatt atttacttaa
     8161 ttatataata tgggtctgta aaagtaaaga attttgtacc attcgaggtt aaagtgcgca
     8221 taaatattgt tttatgtgtt tttttatgcg cttttatatg gcgtatattt tttgatggat
     8281 ttagtataaa gttagatatt ctgtgaaatt tttaaccctg cataaatcaa aacctatttc
     8341 ctaatagtga ttctcaaatt ctaataattc taagttaaaa agatgaaaat tttggagaat
     8401 ataaatatta aaaaattaag tatgaaaaga ttgtcctagc agtaatcttt tcatacttaa
     8461 tttttttaga taatatatca atagcaacta tcattaatat acaatagtta aattttttac
     8521 taactttttt aagtacatta ttctcaaaga ttaatataag aagaatgctt aaactactta
     8581 ctataacaac tttgaaaatt ctattttaaa tgaagttcaa catacacttt tccgaattgt
     8641 gttttatagt attttacacc tgaatttatc taatttttta aaattataaa ccttataatt
     8701 aataatttat ttcacttatt taagttcttt ataagtatat ccttgtgtac tatcttaact
     8761 attttttatt cttggtatat aagtatctat ttttataagg atatatttat ttctcaacaa
     8821 attactattc aaaactttat aattaatatc tgttaaaata atatttattt aatcattcaa
     8881 tagactgtca taatattcta caccaacttc tattttatca gagtctttag gtatcaaacc
     8941 taatgattca caatttattt tatattcttc cctcatattt ttaggagcat ctgcatatgt
     9001 aaatcctata actgaaaaac gtatactgct aacagttcgt aagtcttcaa atcctttagg
     9061 taatacatta agattaacaa tactcgttga attaaccttc aaaaaagtaa tagaagataa
     9121 taaacataaa gttaatgcca ttaatatacc attcttgtac ttttcattaa acatattcat
     9181 aactctcttt ttaatatcag attgcttatg gagtccagct ataaatttat ttgaagtatt
     9241 tgaacctttt ctcattgcat ttagaattgt taaagcataa tccttttttt cctctgaatt
     9301 tcgatttttt aaaactcttt catcacaata aagttcacaa tctaaatcta tatgtttatc
     9361 cattatatat ataaatggat taaaccaata tactgttttt aaaaacagga ttacatattt
     9421 taaataaagg tccctactct taaaatgcat aagctcatgt ctaaaaatcc agtctaactc
     9481 tttcaattta tactcatgtg gtggcaaaag tacacatgaa ttaaaaaatg ctggtgtttc
     9541 aacttcataa gagtatttga gttttatatc ttttgttata ttaaattctt ctaatatatt
     9601 tttataaaga tttacaatct ctctatcatc aacatcataa gataagtcat ttattaaatt
     9661 tttaagtttt atatatttaa atatagtgta aattaaatac act
//
LOCUS       DAMJWP010000130         9662 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.135, whole genome shotgun sequence.
ACCESSION   DAMJWP010000130 DAMJWP010000000
VERSION     DAMJWP010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9662
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.135"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(242..1669)
                     /locus_tag="PC700_002504"
     CDS             complement(242..1669)
                     /locus_tag="PC700_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp26"
                     /protein_id="HDF2581804.1"
                     /translation="MKIKKLLTIGLSLSIFIASCPISANALDKIESIKGADKYETAGI
                     IADKQNYTTAILINADSTMADGLSASGLAGAINAPILLTKKNNIPNATLKRLEKAKKV
                     YIIGGENSINKYTETVLKGKGIEIKRLQGSDRIKTSYNVAKEINSINKVNKVILTNAF
                     KGEPDAMSAAPVAVRDKAAIVLTDGKSVPFNTTGVESYVIGGTSSMNDKLVKDTNSTR
                     LGGTDRYDTNKKIINKFYSGVKEFYIASGTDLVYALVGSTIAKNNAIVLVGNDSNKSV
                     LKSTIKLTAIGNLSDSILQQCLNVTKNIGDSNTGREYTVEDAANAVKKLLGVKKFYYE
                     PNYSEDEDLIFLLDKNFDFSGKEYYVFAYIKQGAEGDVRYCVAKNDMSKVYTYDVNGN
                     MKVCNPDDYKNKGSITEEQAKQIALKECAKRHKVSINNLVVDMIDFRDDIYSGNVYAV
                     KINSKNSNGTFGWFFIDIETGKIYN"
     gene            1860..2681
                     /locus_tag="PC700_002505"
     CDS             1860..2681
                     /locus_tag="PC700_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF2581805.1"
                     /translation="MIISKKITAILVSFLIMISMVGCAPKESATEVAKKYLDSIEIKD
                     YEVAYNLLSKDSKESVSLEKFEEYQDTLFKLQTLKDYKLGEENKVEKYKYSNKEYTNL
                     VKIKETDTIKYITENKEDTANFDRYFIIEDNEYKLLWHKDFKRALSSNYVKIARSETK
                     KDEDKDYNKAAIDIKKAIEIDDKNPLAYYTQSYIYIELQRYDEALKSIDKSLEYLDKN
                     NEAYKIDASDAYNLRGVIFMSKSEFNKARESYNKALELNPDNEYAKTNLNDIKGL"
     gene            2806..4323
                     /locus_tag="PC700_002506"
     CDS             2806..4323
                     /locus_tag="PC700_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF2581806.1"
                     /translation="MSVAIYLRKSRADEEAEKQGEFETLSRHKSTLLKLAKEQNLDVI
                     EIKEELVSGESIIHRPKMLELLKEVEENKYDAVLVMDLDRLGRGDMKDQGIILETFKE
                     SKTKIITPRKTYDLTDEFDEEYSEFEAFMARKELKLISRRMQRGRIKSVEEGNFIGTS
                     APFGYDAVTTGRKERILVPNKDADVVRTIFDLYINEDMGCSKISKYLNNLGIKTATGA
                     NWYNSAITNIIKNKVYCGYIQWQKKDYKKSKNPNKIKTVKLRPKDEWIEAKGKHEPLI
                     SEITWKKAQNILKKNGHVSYGNQIKNPLAGIVICKNCGRPLVYRPYADHDYIICYHPG
                     CNKSSRFEFIEAAILKSLEDTVKKYQLKACDLDLDKNNKDSNIEFQKRVLKGLETELK
                     ELGKQKNKLYDLLERGIYDEDTFIERSNNISSRTEEIKDSIKTVKNRLSTVKKDNSKI
                     IEDIKTVLSLYHDSDSLGKNKLLKSVIDKAIYYKSKEQKLDSFELMVHLKLHEDQ"
     gene            4346..4630
                     /locus_tag="PC700_002507"
     CDS             4346..4630
                     /locus_tag="PC700_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2581807.1"
                     /translation="MCNEISLLDILGTEANYVLDEVELKVQVGKLYEQLNKILTPRER
                     EIVQLRYGLTPYGYKTQREIAQKLDISRSYVSRIEKKALKKLEKELVQES"
     gene            complement(4727..6391)
                     /locus_tag="PC700_002508"
     CDS             complement(4727..6391)
                     /locus_tag="PC700_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDF2581808.1"
                     /translation="MSKKKTPFLKKVGKRSWCIFTIILIIYSVLIYRLVDIQVLKGDK
                     YKQSVESQSVEKVELNSGRGIIYDRNNKKLTDTSKSQVLIVEKEKLNNNYKILELIKK
                     ATKMNDLDIYKAVQEQLTRPIIQIQTKNIDKSMKKELEKNGIMVEEKTMRYAKDGLLS
                     HTIGYIKEDDKSGQSGIEKSMDSVLRNSNEKYISAFKAGDAGNEKSLNILKGSVKTVD
                     NKDKDRHLKTTIDYNIQKKLEQILNKEENPTAAIISEASTGEILAMCSRPNFDQNDIS
                     KSLKGKNGEFENRVIKATYPPGSVFKMVVLFSALENGVIDENYTYNCTGKTKVGNTNE
                     ILRCNKRDGHGFQNLRQAFSNSCNPAFLDIAMKLGKEKILKSAEKLHLFEKVDIGLDE
                     EKIREAPKNISIRNLAIGQENIEFTPLQINQMTQIIANNGTFKPLYLYKSLVDNNMNT
                     IKTYKSSKKEELISPYVCTQVKEYMKSVSRIGTAKDLKDIEGGCGVKTGTAQSSLNKK
                     AIDHGWITGFYPEERPKYVITVLVEGTQKGNKSATPIFKEICESIK"
     gene            complement(6463..7710)
                     /locus_tag="PC700_002509"
     CDS             complement(6463..7710)
                     /locus_tag="PC700_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDF2581809.1"
                     /translation="MELNKVELLAPAGDLERLKIAITYGADAVYIGGEIFGMRSAAKN
                     FSKEDMAEGVAFAHERGKKVFVTVNIIPHNEDFLQLEDYLLELEEIGIDAVIIADPGV
                     LSVIKKVIPNMEIHLSTQANTTNYLSANFWYEHGIKRVVVARELSFDEISEIRAKTPL
                     DMDIEAFMHGAMCISYSGRCLISNYMTGRDANKGSCAQSCRWQYHLVEEKRPGEYFPI
                     YEDERGTFFFNSKDLCMIEYIPELIKSGITSLKIEGRMKTAYYVATVVRAYRMAIDEF
                     YRAPENWKFNPMWMEELKKGSHRHFTSGFYLNKPTTEDQNYQSASYVRNYDFIGIVRE
                     TEDEDGLIVVEQRNKMCVGDEIEVMGPYKETMFTKIEEMYNEEGEAIESAPHPRQIVK
                     LKLSVKVGKDYMLRKVIEEKVEE"
     gene            complement(7697..8371)
                     /locus_tag="PC700_002510"
     CDS             complement(7697..8371)
                     /locus_tag="PC700_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HDF2581810.1"
                     /translation="MSNIVNDLVEDYIRVTLKEKEGFLKDLEIYAEENSVPIIHKEVS
                     DLLKVLLKVQKPKRILEVGCAIGYSSIFFASIIGKDADIITVERNEKMIEKAKENIKL
                     AGFDNNITILEGDAEEKLSQVQGEFDLIFIDAAKGQYKLFFDLVIDKLKDGGLLVSDN
                     ILYKGMVAHDDFVVRRKKTIVKRMRNYLDYICNCDYLSTSLVPIGDGVALSYKESKRG
                     DVDGIK"
     gene            complement(8543..9622)
                     /gene="mltG"
                     /locus_tag="PC700_002511"
     CDS             complement(8543..9622)
                     /gene="mltG"
                     /locus_tag="PC700_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HDF2581811.1"
                     /translation="MNFKENRLKIAVLIIVILIILAGIFVFIQIGPYDKNNKKDVIID
                     VPSGASVGKISDILYENKLIKNELLFKLLVKVSNKAPSIKSGTYLLNQSYSNNDIISL
                     LVSGKIYQDGIKVTIPEGATSKEIIAMLVSKNLGDKATFENLIKKPQEFYDKFPYLKE
                     DGITSLEGFLYPETYYFNSKKQSEEDILSEMLKVFDSKYTDKFKKKQKELNMTLQEVM
                     EMASIIEKEAVLDKDRPIIASVFYNRLKVGMPLQSDATIQYIFEERKKIVTYDDLKID
                     SPYNSYKNKGLPPTPISNPGIKSIEAALYPEKTDYLYFVAKIDGGNNYSTNYQDHLKY
                     VKEYKEARDKQSKDTKATNKENTKK"
BASE COUNT         3292 a         1547 c         1161 g         3662 t
ORIGIN      
        1 gcgagagtgg ctttttgctt ttttgaatag tttactgatt aagtaaacta ccacactagc
       61 tgttaaacta gctaaaacat taaacaaaaa gttatccatg aactcacctc ccttcgaatc
      121 gttgggagga taatcttttg tacatgaact ccactctata gattgtaagt taaatcttct
      181 tgctaaaaat attataacat attatggtaa attatataat tattgtttaa aagttctaat
      241 tttaattata aattttccca gtttctatat ctataaaaaa ccaaccaaaa gtaccattac
      301 tattttttga attgatttta acagcataga cattaccact atatatatca tctctaaaat
      361 ctatcatatc aactactaag ttattgatac tgactttatg tctcttagca cattctttta
      421 atgctatctg ttttgcttgt tcctctgtta tacttccttt atttttataa tcatctggat
      481 tacatacttt catattacca ttaacatcat atgtataaac tttgctcata tcatttttag
      541 ctacacagta tctaacatca ccctctgcac cctgtttaat gtaagcaaat acatagtact
      601 cttttcctga aaaatcaaaa tttttatcta gtagaaatat taaatcttca tcttctgaat
      661 aattaggttc gtaataaaat ttcttcacac ctaatagttt tttcactgca ttagcagcat
      721 cttctactgt atattctctt ccagtattag aatcgcctat attttttgtt acatttaaac
      781 actgctgtaa tatactatca ctcaaatttc ctatagcagt taatttaatt gtacttttca
      841 atacactttt attactatca ttaccaacta acacaatagc attattttta gcaatggtag
      901 aaccaacaag agcatacact aaatcagtac cacttgctat ataaaattct tttactccac
      961 tataaaattt atttattatc tttttgttag tatcatatct atctgtccca cctaatctag
     1021 ttgaattagt atccttaact agtttgtcat tcattgatga agtaccacct attacataac
     1081 tttctacacc agttgtatta aatggaacac tttttccatc agttaatact atagctgctt
     1141 tatctctaac tgctacaggt gcagcactca ttgcatcagg ctctccttta aaagcattag
     1201 tcaatatgac tttatttact ttgttaattg aatttatttc ttttgcaaca ttatagcttg
     1261 tcttaattct atcactacct tgaagtcttt taatctctat tcctttgcct tttagaacag
     1321 tttcagtata tttattaata gagttttctc ctccaattat atatactttt tttgcctttt
     1381 ctaatctttt aagtgtagca tttggtatgt tatttttctt agtcaaaaga attggagcgt
     1441 ttatagctcc tgcaagtcca cttgcactta atccatcagc cattgtactg tctgcattaa
     1501 ttaatatagc tgtagtatag ttttgtttgt ctgcaattat accagcagtt tcgtatttat
     1561 ctgcaccttt aattgattca attttatcaa gagcatttgc agatataggg caactagcaa
     1621 taaatataga taaagataac cctattgtta ataatttttt aattttcata atatctcctt
     1681 ctcttaaata ttttcaccta aattatataa tacttttcag gaaaatgtta ccataaattt
     1741 taaaaattat tattacttaa tataatatta atgaaaaata tcattcttaa attaatgact
     1801 ttggtagaat atggtgataa aataaaatta gtgagagaca atataagggg gattatatta
     1861 tgataatcag taaaaaaata acagcaatat tagtatcatt tttaattatg atatcaatgg
     1921 ttggatgtgc tccaaaagaa agtgctacag aagtagctaa aaagtacctt gatagtatag
     1981 aaatcaaaga ctacgaagta gcatataatt tattaagtaa agacagtaag gaaagtgtca
     2041 gtctagaaaa atttgaagaa tatcaagata ctttatttaa acttcaaacc ctaaaagatt
     2101 ataaacttgg tgaagaaaat aaagtagaga aatacaaata ttctaataaa gagtacacaa
     2161 atcttgttaa aataaaagaa actgatacta taaagtatat cactgaaaat aaagaagata
     2221 ctgcaaattt tgatagatat tttataatcg aagataatga atataaatta ttatggcata
     2281 aagactttaa aagagctctt agctctaatt atgtcaaaat cgctaggtca gaaacaaaaa
     2341 aagatgaaga taaggattat aataaagctg ctattgatat caaaaaagct atagaaattg
     2401 atgataaaaa tccacttgca tattatacac aatcatatat ttatatagag ttacaacgat
     2461 atgatgaggc tttaaagtca attgataaat ccttagaata tctagataaa aataatgaag
     2521 cttataaaat agacgctagt gatgcttaca atttaagagg tgttattttt atgagtaaat
     2581 cagaatttaa taaagctaga gaatcttaca ataaggcgct tgaacttaac ccggataatg
     2641 aatatgcaaa aactaatcta aatgatatta aaggattata aggcattaat attatagaaa
     2701 ataagtatgt tgaaagttaa attaactttt gacatgctta ttttctatat tttaaactct
     2761 attgatttac tatataatag tgcataagga gtgtgattac ttattatgag tgttgccata
     2821 tacttaagaa aatcccgtgc tgatgaagag gcagaaaaac aaggcgaatt tgaaacttta
     2881 agtagacata aatcaaccct actaaaacta gcaaaagaac aaaacttaga tgtaattgag
     2941 ataaaagaag aattagtttc tggtgaaagt ataatacata gacccaaaat gcttgaactt
     3001 ctaaaagaag ttgaagaaaa taaatatgat gcagtcttag taatggactt agacagactt
     3061 ggtcgtggag acatgaaaga ccaaggtatc atattagaaa cattcaaaga aagtaaaacc
     3121 aaaatcatca cacctagaaa aacatacgac ctaacagatg aatttgatga ggagtattca
     3181 gaatttgaag catttatggc aagaaaagaa ttaaaactta tatcaagaag aatgcaaaga
     3241 ggtagaataa aatcagttga agaagggaat ttcataggaa cttctgctcc atttggatat
     3301 gatgctgtaa caacaggtag aaaagaaaga atacttgtac caaataagga tgctgatgta
     3361 gttaggacta tctttgactt atatattaat gaagatatgg gttgtagtaa aatatcaaaa
     3421 tacctaaata acttaggcat taaaactgct acaggtgcta attggtacaa ttctgcaatt
     3481 acaaatataa taaaaaataa ggtatattgt ggctacattc agtggcaaaa aaaggactat
     3541 aaaaaatcta aaaatccaaa taaaataaaa actgttaagc taagacctaa agatgaatgg
     3601 attgaagcaa aaggaaagca tgaaccttta ataagtgaaa taacatggaa aaaagctcaa
     3661 aatatactta aaaaaaacgg tcatgtcagc tatggtaatc aaataaaaaa tccacttgca
     3721 ggaatagtga tttgcaaaaa ttgtggtaga ccattagtat atagaccata tgcagaccac
     3781 gactatataa tttgttacca tcctggttgc aataaaagtt ctcgatttga atttattgaa
     3841 gctgccattt taaaatcttt ggaagacacg gtaaaaaaat atcaattaaa agcatgtgat
     3901 ttagacttag ataaaaataa taaagatagt aatatagaat ttcaaaaacg agtcttaaag
     3961 ggcttagaaa ctgaattaaa agaattaggt aaacaaaaaa ataagttata tgatttatta
     4021 gaacgtggaa tatatgatga agatacattt attgaaaggt ccaacaatat aagttcaaga
     4081 actgaagaaa ttaaagattc aattaagaca gtaaaaaata gattgagtac tgttaaaaaa
     4141 gacaattcta aaataataga agatattaaa actgtgttat cactttatca tgattcagat
     4201 tcccttggaa aaaataaact cctaaaatct gtaattgata aagcaattta ttataagtct
     4261 aaagaacaaa aactagattc ttttgaatta atggttcatt tgaaattaca tgaagaccag
     4321 taataccaat aacatataaa gagtagtgtg taatgaaata agtcttttag atatacttgg
     4381 cacagaggct aactatgttc ttgatgaagt tgaactaaaa gttcaagttg gaaaacttta
     4441 cgaacaacta aacaaaattc ttactcctag agaaagagaa attgtccagt taagatatgg
     4501 gttaactcct tatggctata agacacaaag agaaattgca caaaaactcg atatatcaag
     4561 gtcatatgta tctagaatcg aaaaaaaagc tcttaaaaaa ctagaaaaag agcttgttca
     4621 agaaagttaa tactatattt taaaaggata tatcgtacat cattagacaa agttttctat
     4681 ctaaaatcaa agttacgata tatccttaaa ttttatagta cataatttat tttatacttt
     4741 cacatatttc cttaaatata ggtgttgcag atttatttcc tttttgagtt ccttctacta
     4801 acacagttat tacatactta ggtctttctt caggataaaa tcctgttatc catccgtggt
     4861 ctatagcttt tttatttaaa ctactttgag ctgtaccagt tttaactcca caaccacctt
     4921 caatatcttt taggtcttta gctgtaccaa tcctagatac agacttcata tattctttaa
     4981 cttgtgtaca cacataagga gaaataagtt cttctttttt tgaagactta taagttttta
     5041 ttgtattcat attattgtcc acaagacttt tatataagta tagtggtttg aatgttccat
     5101 tattagctat tatttgagtc atttgattta tttgaagtgg tgtaaattct atattttctt
     5161 gacctatagc taaatttctt attgatatgt tttttggagc ttctctaatt ttttcttcat
     5221 caagaccaat atcaaccttt tcaaataaat gtaatttttc agcagatttg agtatctttt
     5281 cttttccaag tttcatagct atatctaaaa atgcaggatt gcatgaatta gaaaaagctt
     5341 gtcttaagtt ttggaaacca tgaccatctc ttttattaca tcgcagtatt tcattagtat
     5401 tcccaacttt agtcttacct gtacagttgt aagtatagtt ctcatctata acaccatttt
     5461 caagtgcaga aaataaaact accatcttaa atacagaccc tggtggatat gtcgctttta
     5521 taactctatt ttcaaattcc ccattttttc cttttaaact ttttgatatg tcattttggt
     5581 caaaattagg tctagagcac attgcaagaa tttcacctgt agatgcttcc gagattattg
     5641 ctgctgtagg attttcttct ttatttagta tttgttctaa ttttttttga atgttataat
     5701 caatagtagt ttttagatgt ctatctttat ccttattatc aacagttttt acacttccct
     5761 ttagtatgtt tagagatttt tcatttcctg catctccagc tttaaaagca cttatatact
     5821 tttcatttga atttctaagt acactatcca tactcttttc aattcctgat tgacctgatt
     5881 tatcatcttc ttttatatat cctattgtat gtgataataa accatctttt gcgtacctca
     5941 tagttttttc ttctaccatt attccatttt tctcaagttc ttttttcata ctcttatcga
     6001 tatttttagt ctgaatctgt attatagggc gtgtcaattg ctcttgtaca gctttatata
     6061 tatctaaatc attcatctta gtagctttct taataagttc caaaatttta tagttattat
     6121 ttaatttttc tttttcaaca attaatacct gagatttact tgtatctgta agcttcttgt
     6181 tgtttctatc atagattatt cctcttccac tatttaactc gactttctca acgctttgtg
     6241 attcaacact ttgtttgtat ttatcacctt ttaatacttg tatatcaact agcctataaa
     6301 ttaaaacact ataaattatt aaaattatag taaatataca ccagcttctc tttcccactt
     6361 ttttcaaaaa tggagtcttc ttttttgaca tgaaatcacc ccttagataa tttcattatt
     6421 gacaatttct aaggggctaa tactattttt atcaattttt tattattctt caaccttttc
     6481 ttctataact tttctaagca tataatcttt accaactttt actgatagct ttaacttcac
     6541 tatttgtcta ggatgaggag ctgattctat agcttctcct tcttcattgt acatttcttc
     6601 aattttagta aacatagttt ctttatatgg tcccataact tctatttcat caccaacaca
     6661 cattttattt ctttgctcaa caacaataag accatcttca tcttctgttt ctctaactat
     6721 acctataaaa tcatagtttc taacatatga tgctgattga taattttggt cttctgttgt
     6781 tggtttattc aagtaaaaac ctgatgtaaa atgtctatga ctaccttttt ttagttcttc
     6841 catccacatt ggattgaact tccagttttc tggggctctg taaaattcat ctattgccat
     6901 tctgtatgct cttacaacag tagctacata gtatgcagtc ttcattctac cttctatttt
     6961 taaacttgtt ataccagatt tgattaactc tggtatatac tctatcatac ataaatcttt
     7021 tgagttaaag aagaatgtac ccctttcatc ttcgtatatt ggaaaatact caccaggtct
     7081 tttttcttcc accaaatgat attgccatct acaagattga gcacaagaac ctttgtttgc
     7141 atctcttcca gtcatatagt tgcttattaa acatctacca gaataagaaa tacacatagc
     7201 accatgcata aacgcttcta tatccatatc taaaggtgtt tttgctctta tctcagatat
     7261 ttcatcaaaa gataattctc ttgccactac aactcttttt attccatgct cgtaccagaa
     7321 gtttgcactt agataattgg ttgtatttgc ttgtgtactt aaatgtattt ccatattcgg
     7381 aattactttt ttaatgacac taagcactcc tgggtcagct attataactg catctattcc
     7441 tatctcttct agttccaaca aatagtcttc taattgtaga aaatcttcat tatgaggtat
     7501 tatatttact gttacgaata ctttcttgcc tctttcatgt gcaaaagcta ctccctctgc
     7561 catatcttcc ttactaaaat tcttcgctgc agacctcatt ccaaaaattt ctccacctat
     7621 ataaacggca tctgctccat atgttatagc tattttcaat ctctccaaat cacctgctgg
     7681 agctaaaagt tctactttat ttaattccat ctacatcacc tcttttactt tctttataac
     7741 ttagtgctac accatcacct attggtacca atgatgtgct taaatagtca caattacata
     7801 tataatctaa ataatttctc attcttttaa ctatagtctt ttttcttctt acaacaaaat
     7861 catcatgagc aaccatacct ttatacaaaa tattatcaga tactaaaagt cccccatctt
     7921 ttaatttatc tataactaaa tcaaaaaata gcttatattg acctttggct gcatctataa
     7981 atataaggtc aaattcacct tggacttgac taagtttttc ttctgcatct ccttctagta
     8041 tagttatgtt attgtcaaat ccagctaatt ttatgttttc ttttgctttt tctatcattt
     8101 tttcatttct ctcaacagtt attatatctg catcttttcc tataatactt gcaaaaaaaa
     8161 tagaagaata accaattgca catcctactt ctaaaattct ctttggtttt tgaaccttta
     8221 acaagacttt taataaatct gaaacttctt tatgtattat aggaacacta ttttcctctg
     8281 cataaatttc taaatctttt aaaaatcctt ctttttcttt taatgtcact cttatataat
     8341 cttcaaccaa atcattaact atattactca taaaaacact ccctaaaata ataaatattc
     8401 aagttttata ttaatttatt attttttaaa ttcatattgt tatatatatt tacactttct
     8461 ttattataaa tctaagaata acaattttca ataaaaaagc ccaacattat tcttaccctg
     8521 ttagactttt tataatcatg gtttattttt ttgtattttc tttatttgtt gcttttgtat
     8581 cttttgattg tttatccctt gcttctttat attctttaac gtattttaaa tggtcttgat
     8641 agtttgtact ataattattt ccaccatcaa tcttagctac aaaatataaa tagtctgttt
     8701 tctctggata taatgctgct tcaatagatt ttataccagg atttgatatt ggtgtaggag
     8761 gtaatccctt atttttataa ctattatatg gtgaatcaat ttttaaatca tcatatgtta
     8821 caattttctt tctctcttca aatatatatt gtattgtagc atctgattga agaggcatac
     8881 ctaccttaag tctattataa aacacacttg ctattatagg tctgtctttg tcaagaacag
     8941 cttctttctc aattattgat gccatttcca ttacttcttg aagagtcata tttaattctt
     9001 tttgtttctt tttaaacttg tccgtgtatt tactgtcaaa tacttttaac atttcgctta
     9061 aaatatcttc ttctgattgt ttctttgaat taaaataata agtttctgga tacaagaacc
     9121 cttctaaaga ggttattcca tcttccttca aatatggaaa tttatcataa aattcttgag
     9181 gtttttttat taaattttca aaagtagctt tatctcctaa atttttactt actaacattg
     9241 ctataatttc tttagatgta gctccctctg gaatagtcac ttttatacca tcctgatata
     9301 ttttacctga aactaaaaga cttattatat cattgtttga atacgattga ttaagtaagt
     9361 atgttcctga ttttatagac ggagctttat tgctaacctt taccaaaagt ttaaataata
     9421 gttcattctt tattaattta ttttcatata atatgtcaga tatttttcca acagatgctc
     9481 cacttggaac gtcaataatt acatcttttt tgttattttt atcataagga cctatttgta
     9541 taaacacaaa tatacctgct aagataatca aaataacaat tattaatact gctattttca
     9601 atctattttc tttaaagttc ataatataat ctactcctta tgtatattta tccattttct
     9661 at
//
LOCUS       DAMJWP010000131         9660 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.136, whole genome shotgun sequence.
ACCESSION   DAMJWP010000131 DAMJWP010000000
VERSION     DAMJWP010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9660
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.136"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            522..1423
                     /locus_tag="PC700_002512"
                     /pseudo
     CDS             522..1423
                     /locus_tag="PC700_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            1410..2165
                     /locus_tag="PC700_002513"
     CDS             1410..2165
                     /locus_tag="PC700_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2581812.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KSTLLNIISGLIKESSGQVLLNKHNLSNLAEHRRTQIVSRVFQDPSLGTCPSMTVREN
                     LSLALNKGKLLNLRKCLRYKRDFLENLLEGVSLDLKKYLDVQVKFLSGGQRQSLSLIM
                     SCLTSPSVLLLDEHTAALDPKTSNEVIELTNKIVREKNITTLMVTHNLKHALHYGDRL
                     IMLHKGEVVLDVKGKEKEQLTVEEILEKFEYAV"
     gene            2296..3318
                     /locus_tag="PC700_002514"
     CDS             2296..3318
                     /locus_tag="PC700_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2581813.1"
                     /translation="MINKKRLASLILAGALSISMLTGCSQGGDSGNSKQESNSKGKEV
                     KKIGITQLVEHPALDATRIGFVKALEKNGFKDGENIDIDFQNAQNDMPTTQSIASKFA
                     SDKKDLIFAISTPSAQAAFNATKDIPILITAVSDPVAAGLVKTLEKPGTNVSGTSDFV
                     SVDKGLELLKIFAPKAKTIGVMYNTSEVNSKVQVDALKEYASKNGFKVVEKGITTSNE
                     VNQGISSLVGKIDVLYVPTDNLVASSMPIVSKIATENKIPVIAAESGPVEKGALACQG
                     INYEKLGYKTGEMAVKILNGESVSDMPVATSDDTDIIVNEDILKALGMEKPSNENISY
                     VKTKQE"
     gene            complement(3482..4039)
                     /locus_tag="PC700_002515"
     CDS             complement(3482..4039)
                     /locus_tag="PC700_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDF2581814.1"
                     /translation="MTEKEKMLSGKGYYANDELLVKEREYCKKLTRLFNNTLEDEYEK
                     REDILRQLFGSVGKQINVEQNIRCDYGYNIHVGENFFANYDCIFLDVCKIEIGDNVML
                     APNVQIYTAYHPIDAQLRNSGIEYGSPVKIGDNVWIGGGVIITPGITIGDNVVIGAGS
                     VVTKDIPPNTVAVGNPCRVIKKIEE"
     gene            complement(4045..5121)
                     /locus_tag="PC700_002516"
     CDS             complement(4045..5121)
                     /locus_tag="PC700_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HDF2581815.1"
                     /translation="MSLYVDIEKDLSSFKLKVEIKQEKGTLGFLGESGSGKSMTLKCI
                     AGLEKPTRGKIVLNDRVLFDSEKKINLSTQDRKVGFLFQNYALFPHMTVSQNIELGLL
                     KLSKSEKKEIVARYLDILKLNGFEGRYPWQLSGGQQQRVALARALATSPDILLLDEPF
                     SALDHHLRSNMEKELMNMLKDYKGDILFVTHDIEEAYRVCDDIIVYNKGEGLPKRPKK
                     ELFESPKYLIEAKITGCKNISKLNRLDKNTIYATDWGCELTLNREIGDNIEYVGIREH
                     HIKVLDSNEDLNEKLCFELINIVENPFTYTIYVRKTDLSNECVPIQIELEKSKMRFKK
                     GDRIYLDFPQEYLFCFRYNYNKKE"
     gene            complement(5122..5805)
                     /gene="modB"
                     /locus_tag="PC700_002517"
     CDS             complement(5122..5805)
                     /gene="modB"
                     /locus_tag="PC700_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HDF2581816.1"
                     /translation="MGTDWSPLWISLKTSILSTIITFVIGIFVSYIMANYRGKWKGLI
                     DGLFTLPLILPPTVVGFFLLLLCGKNGFIGKFLLNFDRTLIFSWTATVISAVVVSFPM
                     MYRTSRSAFEQIDNNMLSAARTLGLNEWKVFFKIAVPLAWPGIIGGLVLSFARALGEF
                     GATLMIAGNIPGKTQTMPIAIFFAVEGGDMNKAMMWVMIIVAISIAMILLLNYWSEFQ
                     QKIIGKRCG"
     gene            complement(5954..6766)
                     /gene="modA"
                     /locus_tag="PC700_002518"
     CDS             complement(5954..6766)
                     /gene="modA"
                     /locus_tag="PC700_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2581817.1"
                     /translation="MKKILGILGLVACLTLGTVGCNSNEDKTDDGKQEKTTKSSDSVE
                     LNISAAASLKEAMAKIEEEYKKVDSNVKLTVNYGASGSLQQQIEQGAPCDLFISAGQK
                     QMKALDEEKLLVSDTMKDLVKNDLVLISSADSSVSGMKDLTTDKVKKIAVGEVESVPA
                     GKYADEVLTNLNLKDKLKDKLVFAKDVKEVLAWVQSGNADVGFVYFSDTVNNDKIKVV
                     EKTDEKTHSPITYPVSVIKASKNVDAAKKFEEFLLSESGQKIFEEFGYKKVE"
     gene            6959..7888
                     /locus_tag="PC700_002519"
     CDS             6959..7888
                     /locus_tag="PC700_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2581818.1"
                     /translation="MDSLSSLTPVEVAELLKITKNTVYELIKRGELPSYKVGKKIRVD
                     TKDVEEYINSQKTGKVKDTHFNNKNIDINNNFKIIISGQDVILDILARSVEKKLDGIS
                     TFRSYIGSYNALYELYNGRVSIASSHLWDFETDEYNSTFVKKLLPGIPCVLINLAYRM
                     QGFYVAKGNPKEITTWEDLTKPSITIVNREKGSGTRVLLDGKLRLLNFNGKHIKGYDN
                     EELSHLGVASTVSRGIADVGLGNEKAALQVNNIDFIPLHKERYDLVIKKEDLQNPVYQ
                     TIINIINSPEFKAELQGLGGYDLTDTGKTIAKV"
     gene            complement(8049..8498)
                     /locus_tag="PC700_002520"
     CDS             complement(8049..8498)
                     /locus_tag="PC700_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581819.1"
                     /translation="MKNKNNKDKNMLNIKVLRVLGVLLILFDYTGDTLNFYNFFAKPL
                     VSGNLYKTTGTDCIVFLLFIVECVYLVGRYILKVKINWSEIIENEKGNFINNLLVEKI
                     QEESNIVYNLLNSYVLPHIYKKARVLMFKTYHMLIYIKEKYYYVNIY"
     gene            complement(8701..>9660)
                     /locus_tag="PC700_002521"
     CDS             complement(8701..>9660)
                     /locus_tag="PC700_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2581820.1"
                     /translation="CISLRNKFIIISIVFLLILLIVSYIFGKLVYNSSVGLEQLIKNE
                     DIVKIFSERKDKPLEKLKNYKTSELMIQSSNNYKLESLFITSNIKTRDTMILVHGIGS
                     SYYEMLKVAYRYLDKGYNVLVYNQRNTGNSGGDNYTFGLYERYDLDSLVKFVKNKFPE
                     GRLGVHGFSMGAGTAAMHSEINSKDDKVDFYILDSPYSEMKDAIRMGVLEKRIPDILI
                     NYVVICGDLYNKFKSGFWYSDVKPYESVEKSNVPILFIHGTKDTVCNYQNSKKMYDLV
                     KHDKKDLWLIEGIGHVDGFEHDSTVYFNKIFKFIDSNVLSDKS"
BASE COUNT         3341 a         1538 c         1208 g         3573 t
ORIGIN      
        1 ataaaaaaac aataattaag aatataacta tattctacat gtaaaaattt ttttatttaa
       61 actcaatttt ctgataactt gataattttt caaaatttat agttgacttt tattttttac
      121 tgtattaaac taattactat acaaaacaga aaacaaaatt ttcaaaatta ttaaattaaa
      181 aggagaggta aatatgaaga ctttattaaa caaacaagta cgatttttta gtagccaatt
      241 tagttagggt ttgtacctaa gttaagacaa attgtcataa catagataca agccccttag
      301 gattatgatt tttaaggatt gtatctatgt tggctatagt aattgttgtt aaagttttta
      361 ttttatcatc ataatataga gccacatgga ttatactgtg tggcttattc gaatttttgc
      421 catacagtta tatatactgt gtggtttttt tatatcaaaa tcatttttta aaaccaaatt
      481 taaaaaacca tttaaaatat tttcaaaggg gtgttttatt tatgtcaggt attatatctg
      541 taatgacgca aagtttaatt ttatcgatta tggcactggg agtttatata acttacaaaa
      601 tcctagattt tccagacatg tctgctgatg gtagctatac aatgggtgct tctattgtag
      661 cattttctct tactaatggt atttctcctg tagtagcaac tttaatggct atcttatgtg
      721 gttgtactgc tggtcttgtt acagggattc ttcacattaa gtttaagata tctaacttac
      781 tttctggtat tttagttatg ggaatgctat actctatcaa tttaagaata atgggaaaat
      841 caaatattcc tctatttagc tttaaacact tattcaatgg ggaaatatct cctatagttt
      901 tagccttagc ttttgtattc atctgtaaaa tccttttaga tttattccta aaaacaggtc
      961 ttggatacac tttaaaaggt gttggtgata actctcaaat gattaaatct ctgggtataa
     1021 atatcggttc tataaaaata ctaggactta tgatttcaaa cggacttata gctttatctg
     1081 gtagtcttat ggctcagttc ttgggattct cagatgtaaa tatgggtata ggaactttag
     1141 ttcttggaat tgcctctatc ataattggta ttacactatt taagaaattt acttttatca
     1201 aagatactac tgcaataatt gttggctctt ttatatacca attcactata tactttgcaa
     1261 tgagtttagg aatgttatca actgacttaa aactaataac tgccatagtt ataatagctt
     1321 tcctagctac tggtaactta aatatttcac taaaaaaaac aaatgcaaag ctagtaccaa
     1381 aaataaatca gaaaaaagag gtgttgtaga tgttacaaat taaaaatctt tcaaagagtt
     1441 tccctaatcc atatggtgaa cctaatacta tctttgaaaa tttatcaatc gatatagaag
     1501 atggtgaatt tgtaagtatc ataggtagta atggaactgg taaatctact ttattaaata
     1561 taatatccgg gcttataaaa gaatcttctg gtcaagtatt attaaataaa cataacttgt
     1621 ctaatttagc tgaacacaga agaactcaaa tagtaagtag agtttttcaa gacccaagtc
     1681 ttggaacttg cccttctatg actgttagag aaaacttgtc tttagctctt aacaaaggaa
     1741 aactattaaa tctaagaaaa tgtcttcgtt acaaaagaga tttcttagaa aatttattgg
     1801 aaggtgtatc tcttgattta aaaaaatacc ttgatgttca agtaaaattt ttatcaggag
     1861 gtcaaagaca atcattatca ctaattatgt cgtgcttaac aagtccatca gttttattac
     1921 ttgatgaaca tacagcagca cttgacccaa aaacatcaaa tgaagtaatt gaattgacta
     1981 ataagattgt aagggaaaaa aatataacta cacttatggt tactcacaac ttaaaacacg
     2041 ctcttcatta tggtgataga ttgataatgc ttcataaggg tgaagttgta ttagatgtaa
     2101 aaggtaaaga aaaagaacaa ttaacagttg aagaaatctt agaaaaattt gaatatgcag
     2161 tttaaaataa tatatctatt gtattaaact cggctttaaa ataaagttaa aattacaata
     2221 attatggtta ctttgtaata attcatatga atatatgaac atatataata ataaattttt
     2281 gagggggata taaaaatgat taataaaaaa agattggcaa gtttgatttt agctggtgct
     2341 cttagtatat ctatgcttac agggtgttca caaggaggag actctggtaa ttcaaaacaa
     2401 gaaagtaatt ctaaaggtaa agaagttaaa aaaattggta taactcaact ggttgaacat
     2461 ccagcattag atgccactag gataggtttt gtaaaagcat tagaaaagaa cggatttaaa
     2521 gatggagaaa atattgatat agacttccaa aatgctcaaa atgacatgcc tactacacaa
     2581 agtattgcga gtaaatttgc atctgataag aaggacttaa tatttgctat atctactcca
     2641 tcagctcagg cagcatttaa tgctactaaa gatataccta tacttattac tgctgtgagt
     2701 gaccctgttg ctgctggatt agttaaaact cttgaaaagc caggcacaaa tgtatctgga
     2761 acatcagatt ttgtttctgt agacaaaggt ttagaacttc ttaaaatatt tgctcctaag
     2821 gcaaaaacta taggagttat gtacaatact agtgaagtaa attccaaagt tcaagttgat
     2881 gctttaaaag aatatgcatc taaaaatgga tttaaagttg ttgaaaaagg tataactact
     2941 tcaaatgaag ttaaccaagg tatttctagt ttagttggca aaatagacgt attatatgtt
     3001 cctacagata acttagtggc ttcttctatg cctatagtct ctaaaatagc tactgagaat
     3061 aaaatacctg ttatagcagc tgaatctggg ccagttgaaa aaggtgctct tgcttgtcaa
     3121 ggtatcaact acgaaaaact aggttacaaa acaggtgaaa tggcagttaa aattttaaat
     3181 ggcgaatcag tatctgatat gcctgtggct acatcagatg atacagatat cattgttaat
     3241 gaagatatat taaaagcttt aggtatggaa aaaccttcta atgaaaatat ttcttatgta
     3301 aagactaaac aagaatagaa agatattttc tatatacgat taatatgcct gtttctataa
     3361 attaatttat agaaacaggc atatattttt attttaatgt attatctatt taaagattat
     3421 atgtaaatgc tcttaatatg attgtataaa ttttacttgt ataaacttta tacaatcata
     3481 tttattcttc tattttcttt attactctac atggatttcc aacagctaca gtatttggtg
     3541 gtatatcttt tgtgactaca cttcctgcac ctattactac attatcacct atagtaattc
     3601 ctggtgttat gattactccc ccacctatcc atacattatc acctattttt acaggagaac
     3661 catactctat gcctgaattt ctaagttgtg catcaattgg atgataggct gtatagattt
     3721 gaacgtttgg agctaacatt acattgtcac ctatttcaat tttacataca tctaaaaaaa
     3781 tacaatcata atttgcaaag aaattttcgc ctacatgtat gttatatcca taatcacacc
     3841 taatgttttg ttctacattt atttgcttgc caactgaacc aaatagttgt cttaaaatat
     3901 cttctctttt ttcatattca tcctctaaag tattattaaa caatctagtt aattttttac
     3961 aatactctct ttcttttact aacaattcat catttgcata gtatcctttt ccagacaaca
     4021 ttttttcttt ttcagtcata tagtttactc ctttttgtta taattgtatc taaagcaaaa
     4081 taaatattcc tgtggaaaat ctaaatatat tctgtcacct ttcttaaatc tcattttact
     4141 tttttccaat tctatttgaa taggtacaca ttcgttagat aaatcagttt ttctaacata
     4201 tatagtatat gtaaaaggat tttctacaat gttaatcaat tcgaaacata acttttcatt
     4261 taaatcttca ttagaatcca agaccttaat atgatgctcc ctgattccca catattctat
     4321 attatcacct atttctctat ttaatgtcaa ctcgcatccc cagtctgtag cataaatagt
     4381 atttttatcc agtctattta atttggatat atttttacac cctgttatct tagcttctat
     4441 aaggtacttt ggagattcaa agagctcttt tttaggtctc tttggaagac cttctccttt
     4501 gttgtacact ataatatcat cacaaactcg gtaagcttct tcaatatcat gagtaacaaa
     4561 tagtatatct cccttgtaat cttttaacat attcataagt tctttttcca tattacttct
     4621 taaatgatgg tccagtgctg agaaaggttc atctaaaagt aatatatctg gagatgttgc
     4681 caatgctcta gctagtgcta ccctttgttg ttgaccacca gataattgcc aaggatatct
     4741 cccttcaaac ccatttaatt taagtatatc taagtacctt gctactattt cctttttttc
     4801 actcttactt agttttaaaa gcccaagttc tatattttga cttacagtca tatgtggaaa
     4861 caaagcatag ttttgaaata aaaatcctac ctttctatct tgtgttgata aatttatctt
     4921 cttttcagaa tcaaatagaa ctctatcatt taatacaatt ttacctcttg ttggtttctc
     4981 aagccctgct atacatttta atgtcatact ttttccagaa ccagattccc ccaaaaaccc
     5041 cagagtacct ttttcttgtt taatttctac ctttagttta aaagaggata aatctttttc
     5101 aatatccacg tataagctca actatccaca cctctttcct ataatttttt gctgaaattc
     5161 cgaccaataa tttagtaata aaatcattgc tatagatatt gctacaataa tcataaccca
     5221 catcatagct ttattcatat ctccgccttc aacagcaaaa aatatagcta ttggcatagt
     5281 ttgagttttt ccaggtatat ttccagctat cataagtgta gcaccaaatt cgccaagagc
     5341 tctggcaaac gacagcacca atccaccaat tattccaggc catgctaatg gaactgcaat
     5401 cttaaaaaat actttccatt cattaagacc taatgttcta gcagctgaca gcatattatt
     5461 atcaatctgt tcaaaagctg accttgaagt tctatacatc ataggaaaag acaccactac
     5521 tgctgaaatt acagttgctg tccaactaaa tattaaagtc ctatcgaaat ttaataaaaa
     5581 ctttcctata aatccattct tcccacatag taataataaa aagaaaccta caactgttgg
     5641 tggcaaaatt agtggaagtg taaatagtcc atctataaga cccttccact tacctctata
     5701 gttagccata atatatgaca caaatattcc tattacaaaa gttattattg ttgacaaaat
     5761 agatgtctta agtgatatcc ataagggtga ccaatctgtt cccatctttc cctccttaat
     5821 tttaaatgga aatagtgaaa ctaataactc tattaatttc actatttcca ttttattata
     5881 atacacaatt attttcaatt ttcatttatt tatatataat ttatacttaa ttacacataa
     5941 tttatttata agtttattct acttttttat atccaaattc ttcaaaaatc ttttgtcctg
     6001 actcacttaa taaaaattct tcaaatttct tagcagcatc tacgttttta ctagctttaa
     6061 ttactgaaac tggatatgta ataggtgaat gagttttttc gtctgttttt tcaactactt
     6121 ttatcttatc attattgaca gtatcactga agtaaacgaa tccaacatca gcatttcctg
     6181 attgcaccca tgctaaaact tcttttacat cttttgcaaa tactagctta tcttttaact
     6241 tatcttttaa atttaaatta gttaacacct catctgcata cttaccagct ggaacacttt
     6301 caacttcacc aacagcaatt ttcttcactt tatctgtagt caaatctttc atacctgata
     6361 cactactgtc agcagaagaa ataagaacta aatcattttt tactaaatct ttcatagtat
     6421 cagaaactaa taacttctct tcatccaaag ctttcatttg tttttgacct gctgaaataa
     6481 ataaatcaca aggtgctcct tgttctattt gttgttgtaa tgagcctgat gctccatagt
     6541 taactgtaag ttttacattt gaatcaactt ttttatattc ttcttcaatc tttgccattg
     6601 cttcttttaa acttgcagca gccgatatat ttaactctac tgaatcacta cttttagttg
     6661 ttttttcttg ttttccatca tctgttttgt cttcattact attgcaccca actgtcccca
     6721 aagttaaaca tgcaactaat cctaatattc ccaatatttt tttcataaca atctcccttt
     6781 ttgtaaattt gatacacatt ttaatatgtt ttattatgtt ttatcatgtt ttgttatata
     6841 tagtttaatt atatatacat gataataaat tttcaagtaa tttatttaca ctacttttaa
     6901 atattttgct acattttcct ttttttaatg ttaaaataac cttatatgga ggtggattat
     6961 ggactcatta tcatctttga cacctgtcga ggtagctgaa ttattaaaga taacaaaaaa
     7021 tactgtatat gaattaatca aaagaggtga acttccttct tataaagtag ggaaaaaaat
     7081 aagagttgat actaaagatg ttgaagaata tatcaacagc caaaaaactg gtaaagtaaa
     7141 ggatacacat ttcaacaata aaaatataga tataaataat aatttcaaaa ttattatttc
     7201 tggtcaagat gtaatattag acattttagc taggtctgtt gaaaaaaaat tggatggaat
     7261 aagtacattt cgctcttata taggtagtta taatgctttg tatgagttat ataatggcag
     7321 agtttctata gcatcatccc atctatggga tttcgaaacg gatgaatata atagcacttt
     7381 tgttaaaaag ctattgccag gaataccatg tgtacttata aatctagctt atagaatgca
     7441 aggtttctat gttgctaaag gaaatcctaa agagataaca acctgggagg atttaacaaa
     7501 accaagtata accattgtaa atagggaaaa aggttctgga actagggttt tactagatgg
     7561 taaacttaga cttctaaact ttaatggtaa acatataaaa ggttatgata atgaagaatt
     7621 atcacatctt ggagttgcaa gtactgtttc tcgtggaata gctgatgttg gtttgggaaa
     7681 tgaaaaagct gctttacaag taaataatat cgactttatt cctcttcaca aagagcgcta
     7741 tgatttagtt attaaaaaag aagacttaca aaatccagtt taccaaacta taattaacat
     7801 aataaactct cctgagttta aagctgaact tcaaggtctt ggcggatatg atttaactga
     7861 tactggaaaa actatagcaa aagtttaaga ttaaaatcgc aatctatttt ataagattta
     7921 taatttttca caaaatttat aacttaaaca cgaaataaat tgcgatttta gtgtctcaaa
     7981 aaatttatgt ctttcatttt atcatccaat acaaaatata atataattaa ttgtgcaatc
     8041 atattatatt aatatatatt tacataatag tatttttctt taatgtagat taacatatga
     8101 tatgttttaa acatcaacac ccttgctttc ttatatatat gtggcaacac atatgagttt
     8161 aataaattat atacaatgtt gctttcttct tgtatttttt caaccaacaa gttatttata
     8221 aaattacctt tctcattttc aataatctca ctccaattta tcttaacttt taatatatat
     8281 cttcccacta ggtatacaca ctctacaata aataataaaa atacaataca gtcagtacct
     8341 gttgtcttat ataaattacc cgatactaat ggctttgcga agaaattata aaaattcaaa
     8401 gtatcacccg tataatcaaa aagtattaat agcactccta gaactctcaa tactttaata
     8461 ttcaacatgt ttttatcttt attatttttg tttttcatat atactacctc tcttttcttt
     8521 agatttgatt gtaatctaat actaactctt ttatttgagg tataaatgaa tttaattggt
     8581 agttattttc atttttacta gaaattaatt cacattacta aacaaaactt atatttaaac
     8641 ataaaaatag cctatctatt ttttaataaa tacaaaaata aataagctac taatattcaa
     8701 ttaactttta tcacttaaaa cattactatc tataaattta aatattttat tgaagtatac
     8761 tgtactatca tgttcaaatc catcaacatg accaatacct tctataagcc ataggtcttt
     8821 tttatcatgt ttaactaaat catacatttt cttgctattt tgataattac aaacagtatc
     8881 ttttgttcca tgtataaaca gtatgggtac attagatttc tcaactgact catatggttt
     8941 tacatcactg taccaaaatc cagatttaaa cttattatat aaatctccac aaatcaccac
     9001 ataattgatt aatatatctg gtattcgttt ttctagtact cccattctaa ttgcatcttt
     9061 catttcagaa taaggagaat ccagtatata aaaatcgacc ttatcatctt ttgaatttat
     9121 ctctgaatgc atagcagctg tccctgctcc catggaaaat ccatgcactc ctaatcttcc
     9181 ttcaggaaat ttatttttta caaatttaac tagactatct aaatcatacc tttcataaag
     9241 accaaatgtg taattatctc caccactatt accagtattt ctttgattat aaactaatac
     9301 attataacct ttatctaaat atctatatgc tacctttaac atttcatagt aactacttcc
     9361 aattccatga acaagtatca tagtatctct tgtctttata tttgaagtta taaaaagact
     9421 ttcaagcttg taattattag aactttgtat cattaattca gatgtcttgt agttttttaa
     9481 tttctccagt ggtttatctt ttctctcaga aaatattttt actatatctt cgttttttat
     9541 taactgctct aaaccaaccg aactattata cactaatttg ccaaatatat aagatacaat
     9601 taataggatt aatagaaaca caattgatat aattataaat ttatttctta aactaataca
//
LOCUS       DAMJWP010000132         9493 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.137, whole genome shotgun sequence.
ACCESSION   DAMJWP010000132 DAMJWP010000000
VERSION     DAMJWP010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9493
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.137"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            33..1397
                     /locus_tag="PC700_002522"
     CDS             33..1397
                     /locus_tag="PC700_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2581821.1"
                     /translation="MGEKKINNMNYSHNNYSKKTGEPTRKIKNSHITLKSKEEKICSS
                     CNKENNTKDNYCKFCGNELYEIASLRPLETKLDLKSKIKELSYHANKRGVFLTTFTTI
                     FILFIIALIFKAIITIQFNDISYLINPAHIILALNLGQISVSMSTMMGSGFINANIGL
                     LILLIIPILVLLISNLIFMRKRCRDSKTVLANSLGVGIFYGLVLAILSIFTNVKTSSH
                     SMLEYGYALEYSYEFFSVLLNGFVLGFICTYITTYKKSYEKENMYLSLFSNSIRTFIL
                     GYVLVLVILLVLTISDSSYLNELDMSSYSNGLNLFTILPQIASYMWAFANGISVTIIN
                     STVSMFTLSSSSLFGDTKLMFYAMGALSMLILLLNGYKLRFKYNTDSIRPIIVFSIYY
                     AFLMGILALFSTFILDSNINFFNTTNYGTTLIMQFKVLQAIVISFVYSFVISLIGYKL
                     NSAD"
     gene            1419..1910
                     /locus_tag="PC700_002523"
     CDS             1419..1910
                     /locus_tag="PC700_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2581822.1"
                     /translation="MEGNTNNKVDDKFSKVKKKFGSGKFKVELYKEKSKLNREIDDIQ
                     EKKAKIFLEMGILTYQKIREDSINDNSFDKFCDELLELDKLIYEKNMEINEMEMAESN
                     VTCECGYVGNVNDKYCAECGKKFELENECDDFIICGYCESEIDTEAEFCPCCGRKIIM
                     DLE"
     gene            2183..2854
                     /locus_tag="PC700_002524"
     CDS             2183..2854
                     /locus_tag="PC700_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDF2581823.1"
                     /translation="MLDKVKGRLIVSCQALENEPLHSPFIMGRMAKAAMEGGAVGIRA
                     QGVEDIIEIKKVTGLPVIGIIKRNYEDSDIYITPTKKEVDELLTTGCEMIALDATNRV
                     RPNNEDLKELIKYIKENGVLVMADISNYDEAIKAQEYGVDCVSTTLSGYTPYTKTLEG
                     PDFVLMERLVKDLEIPVIAEGKVNTPQDLKKVFELGVHSSVVGSAITRPQLITEKFVK
                     AIEIN"
     gene            2939..3805
                     /locus_tag="PC700_002525"
     CDS             2939..3805
                     /locus_tag="PC700_002525"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HDF2581824.1"
                     /translation="MKGIYSALLVSFDKEGNINEKGLRQIIRHNIDVCKVDGLYVGGS
                     TGENFMLSTDEKKRIFEIAKDEVKEEIKLIAQVGSVNLKEAVELAKFTTDLGYDAISA
                     VTPFYYKFDFEEIKHYYNTIINSVDNRLIIYSIPFLTGVDMSLDQFGELFENEKIIGV
                     KFTAADFYLLERMRKTFPNKLIFAGFDEMMLPATVLGVDGAIGSTFNVNGVRARQIFE
                     LTKNEKISEALEVQHVTNDLITDILGNGLYQTIKLLLEEQGVEAGYCRQPMKEATDEM
                     KSRAKEIYRKYF"
     gene            3838..5340
                     /locus_tag="PC700_002526"
     CDS             3838..5340
                     /locus_tag="PC700_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HDF2581825.1"
                     /translation="MVGFTWIDFAILVVYLLAVLFAGLLFSKKEMKGKEFFKGDGTIP
                     WWVTSVSIFATLLSPISFLSLAGNSYGGTWILWFAQLGMFIAIPLTIKFFLPLYSRLE
                     IDTAYEYLEMRYESKGLRVLGALMFIIYQIGRMSIIMYLPSVVLAGLTGIPVNVLIVV
                     MGVIAIIYSYTGGLKAVLWTDFIQGMVLIVGVTGTLFYLIASINGGFGTVMDTLTTGH
                     KFLASNEVMFDKNILSTSAFIIFIGAGLNTFSSYVSSQDIVQRFTTTTDIKQLNKMTY
                     GNGVLSMGLATVFYLIGTCLFIYYTQNPDLAQTVQQDQVFASYIAYELPVGITGILLA
                     AIYAASQSTLSTGLNSVATSWTLDIQTIITKDISMEKQTKIAQYISLGVGILSIAVAI
                     VLANGEIKSAYEWFNSFMGLVLGVLAGIFVLGAFCKKATKMGAYVGFIVSAILVVYLK
                     YRMPEVTSWAYSLITITTSVVVGTIVSAIEAKVKAKVSLPKAETTVYYEK"
     gene            5470..5922
                     /locus_tag="PC700_002527"
     CDS             5470..5922
                     /locus_tag="PC700_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDF2581826.1"
                     /translation="MIFGNINHSKTYSTLHEDLLRCFEYAKDNSLIDYEKGTYTIDGD
                     DIFVNIVEYKTCEKEDRFWEAHKKYIDVHLMLDGCERIDINFIENLEQKIYEEKGDFL
                     PLDGKNNGYMELRKGDFLVCYPEDAHMTGIKVLEKENIKKAIFKVLVR"
     gene            6082..6885
                     /locus_tag="PC700_002528"
     CDS             6082..6885
                     /locus_tag="PC700_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581827.1"
                     /translation="MENIDVFLLIGQSNARGLGNPKESIIPNENCFEYLSTDEIINMR
                     CELETSEGDGTIAPAFSNEWNKLTGNKVCFIHNAKDGSRIKNWNHDNNWFLNDTIEKF
                     NTGCTTLSKHYNIENKYVIWIQGESDAKYGSDALYYKESLKKIAYRLKEECMIDKMFV
                     SLTGYWLGEDEYFIRTRRIAAAQESACNECDILCVGSKIAMQFHDRGLTIDDVHYTQE
                     GLNMLGEDLCKNIYKYHITKEKSVLSDTIDLSEARKYICELEKISKRFV"
     gene            6943..7830
                     /locus_tag="PC700_002529"
     CDS             6943..7830
                     /locus_tag="PC700_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDF2581828.1"
                     /translation="MKNYVCIDVGGTSIKYGFIREDGFIIDKSSLDTEAKEKGGEGIL
                     AKIKDIVKKYIEENEISGICISTAGMVDPVEGKILFALEELIPNYKGMQLKKEVEKEF
                     NIKCEVENDVNCAGLGEVWLGAGRGATSSICLTIGTGIGGCIIINNKLINGFSNSAGE
                     VGYMNINGSSFQELASTSSLIKKVAKIKNLNEKDLNGKIIFDLAKNNDEDCLKELDNM
                     IKSLAVGIANICYVINPEVVILGGGIMAQEKFLKPKIDKALKEVLIERVYKNTNIEFA
                     KRQNDAGMLGALYNFLNKI"
     gene            7849..8697
                     /locus_tag="PC700_002530"
     CDS             7849..8697
                     /locus_tag="PC700_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2581829.1"
                     /translation="MSILEQLESPTFKVTKSDKLLIAYIKENIDDVFYKPISQIAKES
                     NIGEATITRFVKKMNFNGLQDFKVTLAQEISTINKKNIINKNIQNDEPALDTAKKLLS
                     SNVTTLENTVEIINSKDVHDCARLIINAKKVYFIGIGYSGIIAQDSNYKFMRIGLNCV
                     SFDSSHTMIMMSSIMEEGDLIIAISHSGETEEIIKTVKLARANNAKIISITENKNSEL
                     KDISDVHLSYVSGETVLETGSISSKLAQFFIIDLVYTQVVKELSNEAIERKIKTTNAI
                     KLFKNE"
     gene            8760..9449
                     /locus_tag="PC700_002531"
     CDS             8760..9449
                     /locus_tag="PC700_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF2581830.1"
                     /translation="MSKLKDYNIDKVIIFNNLSTKTKNQLKEKAKLKRLQKKEILFYE
                     KDSLDSVYILLEGKVSIFKISENGERKVIFILNSGEVINDITFDSSKNSTVGCEAFEN
                     SIVAYYSIEEFLEIMQNDFQLTKDIISYMERRIRRLYRQLKNSISIKVDKKLAAKLYR
                     LSREFGVCCGEWTLLNVNITVTYLADMLGCKRETVSRMIKVLQKEELIKIDNKGFYVK
                     ETELSRYFKNN"
BASE COUNT         3819 a          900 c         1673 g         3101 t
ORIGIN      
        1 taatttacaa tagtaaaact tggagggaaa aaatgggtga aaagaaaata aataatatga
       61 attattcaca taataattat tccaaaaaga caggtgaacc aactagaaaa ataaaaaata
      121 gccatatcac tcttaaaagt aaagaagaaa aaatttgttc tagttgcaat aaagaaaata
      181 atactaagga taattactgt aaattttgtg gaaatgaact ttatgaaata gcatcattaa
      241 gaccacttga aacaaaacta gacttgaaat ctaaaataaa agagctttca tatcatgcaa
      301 ataaaagagg tgtattttta acaacattta caaccatatt tatattgttt attattgcat
      361 tgatatttaa agctattata actattcaat ttaatgatat tagttattta ataaatccag
      421 cacatataat cctagctctc aacttgggtc aaattagtgt atctatgtct accatgatgg
      481 gttctgggtt tataaatgcc aatataggtt tgttgatatt acttataatc cctattttgg
      541 tattattaat atcaaaccta atttttatga gaaaaagatg tagagattca aaaactgtat
      601 tagcaaattc tctaggagtt ggaatttttt atggattagt tttagctatt ttatctattt
      661 ttactaatgt aaaaactagt tcacatagta tgttagaata tggatatgca ttagaatata
      721 gttatgaatt ttttagtgtt cttttaaatg gatttgtatt agggtttata tgtacataca
      781 taactactta taaaaaaagt tatgaaaagg aaaatatgta tttatctctt ttttcaaatt
      841 ctataagaac attcatatta ggttatgttt tagtattagt aatactattg gtactaacta
      901 tatctgatag tagttactta aatgaactag atatgagtag ttattcaaat ggattaaatt
      961 tatttactat actcccacaa atagctagtt atatgtgggc ttttgcaaat ggaatttcag
     1021 taaccataat aaactcaaca gtatcaatgt ttactttaag ttcttcatct ttatttggag
     1081 atactaaatt aatgttttat gcaatgggag cattgtctat gttaatatta ttattaaatg
     1141 gatacaagct aagatttaag tataatacag atagtataag acctataata gtatttagta
     1201 tatattatgc atttttgatg ggaatattgg ctttatttag tacatttata ctagatagta
     1261 atattaattt ctttaataca actaattatg gaacaacttt aataatgcaa tttaaagtct
     1321 tacaagctat tgtaatttca tttgtatata gttttgtgat atctttaata ggatataagc
     1381 taaattcagc tgattaaata tgattggagg gaatttctgt ggaaggaaat actaataata
     1441 aagtagatga taaattttct aaggttaaga aaaagtttgg tagtggaaaa tttaaagttg
     1501 aattatataa agaaaaatca aaattaaata gagagattga tgatattcaa gaaaagaaag
     1561 caaagatatt cttggaaatg ggaatactga catatcaaaa aattagagaa gattctatta
     1621 atgataatag ttttgataaa ttctgtgatg agttactaga acttgataag ttaatttatg
     1681 aaaaaaatat ggaaattaat gaaatggaaa tggcagaaag taatgtaact tgtgagtgtg
     1741 gttatgttgg aaatgtaaat gataaatatt gtgctgaatg tggtaaaaaa tttgaattgg
     1801 aaaatgaatg tgatgatttt ataatttgtg gttattgtga aagtgagata gatacagagg
     1861 ctgaattttg tccatgctgt ggaagaaaaa ttataatgga tttagaataa tattgggaaa
     1921 cctatgtata gtatacatgg gtttttttat tgaaattatt taaatggact tataacagta
     1981 gttatataaa atgaaaataa acaccaaaaa aatgaaataa aatttctttt aaatgtaaaa
     2041 aacaagctaa tatataaaat aaatcagtaa aaatatagat gcgagaaaaa aatatgaaaa
     2101 aatattgcaa agaaaaagtt ttcatgttat tattaataca taaatcaaga aaataaattt
     2161 cagaaacgag gtaatttaaa aaatgctaga taaggtaaag ggaagattaa tagtatcatg
     2221 ccaagcttta gaaaatgaac ctcttcatag tccttttata atggggagaa tggctaaggc
     2281 tgctatggaa ggtggagctg ttggaattag agctcaagga gttgaagata ttattgaaat
     2341 aaaaaaagtt actggacttc ctgttatagg tattataaag agaaactatg aagattcaga
     2401 tatatatata acgccaacta aaaaagaagt tgatgaactt ttaacaacag ggtgtgaaat
     2461 gatagcatta gatgctacca atagagttag accaaataat gaagacttaa aagaacttat
     2521 aaagtatata aaagaaaacg gtgttctagt aatggcagac atttcaaatt atgatgaggc
     2581 tatcaaagca caagagtacg gtgtagattg cgtttcaact acactatctg gatatactcc
     2641 atatactaaa acattagaag gtccagattt tgtattaatg gaaagattag ttaaagattt
     2701 agaaatacct gtaatagctg aaggaaaggt aaatactcct caagatttaa aaaaggtctt
     2761 tgagttgggt gttcattcat cagtagtagg ttcagcaata acaagaccac aattaataac
     2821 agaaaaattt gttaaagcaa ttgaaattaa ttaaaaatta atttataaat taaaaaatta
     2881 ttatatttaa ctatacttga aaattaaaat tggaggtaaa gatatgagaa caacagatat
     2941 gaagggaatt tattcagcat tattagtttc atttgataag gaagggaata taaatgaaaa
     3001 agggttaaga caaataatta gacacaatat agatgtttgt aaagtagatg gtctttatgt
     3061 aggaggaagt acaggggaaa acttcatgct ttcaactgat gaaaagaaaa gaatatttga
     3121 aatagctaaa gatgaagtga aagaagaaat aaaattaata gctcaagttg gttcagttaa
     3181 tttaaaagaa gctgtagaac ttgctaaatt tacaactgat ttaggatatg atgcaataag
     3241 tgctgttaca cctttttact ataaatttga ttttgaagaa ataaaacact attataatac
     3301 aataataaat tctgtagata atagattaat aatatactct ataccatttt taactggtgt
     3361 tgatatgagt ttagaccagt ttggagaatt atttgaaaat gaaaaaataa taggtgttaa
     3421 gtttacagca gcagatttct acttattaga gagaatgaga aaaacattcc caaataaatt
     3481 aatatttgca ggatttgatg aaatgatgtt accagctaca gttttaggtg tagatggggc
     3541 aattggttct acattcaatg taaacggtgt cagagctaga caaatattcg aattaactaa
     3601 gaatgaaaaa atatcagaag cacttgaagt acaacatgtg actaatgatt taataacaga
     3661 tatattagga aatggcttat atcaaacaat taaattacta cttgaagaac aaggtgttga
     3721 ggctggatac tgtagacaac ctatgaaaga agcaactgat gaaatgaaat caagagcaaa
     3781 agaaatatac agaaagtatt tttaataaaa tacttttagt atgggaggat tagtcaaatg
     3841 gtaggattta cttggataga ttttgcaata ctagtggttt acttattggc agttttattt
     3901 gcaggattgt tattttcaaa aaaggaaatg aagggaaaag agttcttcaa aggtgatgga
     3961 actattccgt ggtgggttac atcagtatca atatttgcca cattattaag tccaatatca
     4021 tttttatcat tagcaggaaa ttcttatggt ggtacttgga ttttgtggtt tgcacaatta
     4081 ggtatgttta tagcaatacc tttgacaata aaatttttct tacctctgta tagtaggtta
     4141 gaaatagata cagcatatga atacttagaa atgagatacg aaagtaaagg tcttagagta
     4201 cttggagctt tgatgtttat aatatatcaa ataggacgta tgtctatcat aatgtatctt
     4261 ccatcagtgg ttctagctgg tcttacagga atacctgtaa atgtattgat agtagttatg
     4321 ggtgtaatag caataattta ttcatataca ggtggattaa aggctgtctt gtggacagat
     4381 tttatacaag gtatggtatt aatagtaggt gttacaggga cattgttcta tttaatagca
     4441 agtataaatg gtggttttgg aacagtaatg gatactctta caactggaca caagttctta
     4501 gcaagtaatg aagttatgtt tgataaaaat atactatcta caagtgcatt tataatattt
     4561 attggagctg gtcttaatac tttttcatca tatgtatcga gtcaagatat agttcaaaga
     4621 tttactacaa caactgatat aaagcaactt aataaaatga catatggaaa tggtgtttta
     4681 tcaatgggac ttgcaacagt attttatcta attggaactt gtttatttat atactatact
     4741 caaaacccag atttagctca aacagttcaa caagaccaag tatttgcatc atatatagct
     4801 tatgaattac cagtgggtat aacaggaata ctacttgcag cgatatatgc agcttctcaa
     4861 tcaactttat cgacagggct taattcagtt gctacaagtt ggactttaga tatacaaact
     4921 atcataacaa aagatataag tatggaaaag caaactaaga tagcacaata tatatcatta
     4981 ggtgtaggta tactttctat agctgttgca atagtattag caaatggaga aataaagtca
     5041 gcatatgaat ggtttaatag ttttatgggg ctagtactag gtgttttagc aggaatattt
     5101 gtacttggtg cattttgtaa aaaagcgact aaaatgggag catatgttgg attcatagta
     5161 tcagcaattt tagttgttta tttaaaatac cgtatgccag aagtaacttc ttgggcatat
     5221 tctctaataa caatcacgac ttctgtagtg gtaggtacaa tagttagtgc aatagaagct
     5281 aaagttaaag caaaagtatc tttaccaaaa gctgagacta ctgtttatta tgaaaaataa
     5341 tatttaaatg ataaattgtc ttatagagtg attagataaa taatttagtt actctataag
     5401 attgtataat atactttgta tataaaagta taaaaataga ctttataatt atatagggag
     5461 atagtgctta tgatatttgg aaatataaat catagtaaga catattcaac attgcatgaa
     5521 gatttactta gatgttttga atatgctaaa gataattcat taatagatta tgaaaaaggt
     5581 acttatacaa tagatggtga tgatattttt gtgaatattg tagagtataa aacttgtgaa
     5641 aaagaagata gattctggga agctcataag aagtatatag atgttcattt gatgcttgat
     5701 ggatgtgaac gaatagatat aaattttata gaaaatttag aacaaaaaat atatgaagaa
     5761 aagggcgatt tcttgcctct tgatggtaaa aataatggat acatggaact tagaaaaggt
     5821 gattttttag tttgttatcc agaagatgct catatgacag gaataaaagt tttagaaaaa
     5881 gaaaatatca aaaaagctat ttttaaggtt ttggttagat aatgataatt tatataacca
     5941 ctagggctga aaatagtgat ttaactttca gcgattagtg agacttcgta aattttagtt
     6001 agattactat tttcagcctt agttttttat gagaaaattg aaaataatta aaagcttaaa
     6061 attttgtaaa ggagttaact tatggaaaat atagatgtat tcttattaat aggacaaagc
     6121 aatgctagag gattgggaaa tccaaaagag agcatcattc caaatgaaaa ttgttttgaa
     6181 tatttgagta ctgatgaaat tataaatatg agatgtgagc tagagacttc agaaggtgat
     6241 ggtactatag cacctgcatt ttcaaatgaa tggaataagc ttactggaaa taaagtttgt
     6301 tttattcaca atgcaaaaga tggttcaaga ataaaaaact ggaatcatga taataactgg
     6361 tttctaaatg acactataga aaaatttaat acaggctgta ctaccttaag taaacattat
     6421 aatatagaaa ataaatacgt tatttggatt caaggtgaaa gtgatgcaaa atatggtagt
     6481 gatgcattgt actataaaga aagtctaaaa aaaattgcgt ataggcttaa agaagaatgt
     6541 atgattgata aaatgtttgt aagtttaact ggttattggc ttggagaaga tgaatatttt
     6601 attagaacaa gacgaattgc agcagctcaa gaatctgctt gtaatgaatg tgatatactg
     6661 tgtgtaggaa gtaaaattgc tatgcaattt catgacagag gattaactat tgatgatgtt
     6721 cattacactc aagaaggtct taatatgtta ggagaggact tatgtaaaaa tatttataag
     6781 tatcatataa ccaaagaaaa aagtgtatta agtgatacta tagacttatc agaagcaagg
     6841 aaatatatct gtgaattgga aaagataagt aaaagatttg tgtaatataa aattttaaat
     6901 agagttgtta atttataaaa ataatttgta tggagatatg ttatgaaaaa ttacgtttgt
     6961 atagatgttg gaggtacttc aataaaatat ggttttatta gagaagatgg atttataatt
     7021 gataaatcaa gtcttgatac tgaagcaaag gaaaaaggtg gagaaggaat attagccaaa
     7081 ataaaagata tagtaaaaaa atatatagag gaaaatgaaa taagtggtat atgtatatca
     7141 actgcaggta tggttgaccc agttgaaggt aaaatactat ttgcattaga agaattaatt
     7201 ccaaattata agggtatgca attaaaaaaa gaagtagaaa aagagtttaa tataaaatgt
     7261 gaagttgaaa atgatgttaa ttgtgctgga cttggtgaag tgtggcttgg cgcaggtaga
     7321 ggtgcaacat cttctatatg tttaacaata ggtactggaa ttggtggatg tattataata
     7381 aacaataaac tgataaatgg gtttagtaat agcgctggtg aagtaggata tatgaatata
     7441 aatggaagtt cttttcaaga attggcatct actagcagtc taattaaaaa agtagcaaaa
     7501 ataaaaaatt tgaatgaaaa ggacttaaat ggaaaaataa tttttgattt agcaaagaat
     7561 aatgatgaag attgtttaaa agaactcgat aatatgataa aatcattagc agttggaatc
     7621 gcaaatatat gttatgtaat aaatccagaa gtggtaattt taggtggtgg gattatggca
     7681 caagaaaagt tcctaaaacc aaaaatagac aaagcattaa aggaggttct tatagagaga
     7741 gtttacaaaa acactaatat agaatttgca aagagacaaa atgatgcagg aatgcttgga
     7801 gcattatata attttttaaa caaaatataa agaatagaag ggtttttaat gagtatttta
     7861 gaacaattgg aatcaccaac ttttaaagtc acaaaatcag ataaattatt aatagcttat
     7921 ataaaagaaa atatagatga tgtattttat aaaccaatat cacaaatagc taaagagagc
     7981 aatattggtg aagcaactat aactaggttt gtaaaaaaaa tgaattttaa tggattacag
     8041 gattttaaag ttactttagc acaagaaata tcaactatca ataaaaaaaa tattataaat
     8101 aaaaatatac aaaatgatga gccagctttg gatacagcaa aaaaactttt aagttcaaat
     8161 gtaaccactt tagagaacac agtagagata atcaatagta aggatgtaca tgactgtgca
     8221 aggcttataa taaatgcaaa gaaagtttac ttcataggca ttggatattc tggtataata
     8281 gcacaggatt caaactataa atttatgaga ataggcctta attgtgtaag ttttgatagc
     8341 agtcatacga tgataatgat gtcttccata atggaagaag gagatttaat aattgcaatt
     8401 tcacattctg gtgaaacaga agagattata aaaactgtaa agcttgcaag agccaataat
     8461 gctaaaatta tatcaattac tgaaaataaa aattctgaat taaaggatat ttcagatgta
     8521 cacttatctt atgtatcagg agaaacagta ctggaaactg gttcaatatc ttctaaatta
     8581 gcacaatttt tcattattga tttagtatat acacaggtag taaaagaact ttctaatgaa
     8641 gctattgaaa ggaaaattaa aactacaaat gctataaaat tgtttaaaaa tgaataaaat
     8701 aagaaatttg taatgtttta tagagctctc ttaaaaattt aggaatggag agatattaca
     8761 tgtctaaatt aaaagattat aatattgata aagtaataat ttttaataat ttaagtacta
     8821 aaacgaagaa tcaacttaaa gaaaaagcta aactaaaaag attacaaaaa aaagaaattt
     8881 tattttatga aaaagatagc ttagacagtg tttatatact tttagaagga aaggtatcta
     8941 tttttaagat aagtgaaaat ggagagagaa aagtaatttt catattaaat agtggggaag
     9001 tcataaatga tattacattt gactcttcta aaaactccac tgttggctgt gaagcatttg
     9061 aaaattcaat tgtagcttat tatagtattg aagaattttt agaaattatg caaaatgatt
     9121 ttcaacttac aaaggatatt atttcttata tggaaagaag aataagacgt ttatatagac
     9181 agttgaaaaa ttctatatcc ataaaagtag ataaaaaact tgcagcaaaa ttatataggt
     9241 taagtagaga atttggagtt tgctgtggag aatggacttt actaaatgta aatattacag
     9301 ttacatattt agctgatatg cttggctgta aaagggagac tgtttccaga atgataaagg
     9361 tattgcaaaa agaagaactt attaagattg ataataaagg tttttatgtt aaagaaactg
     9421 aattatcaag gtatttcaaa aataattaat tatgattaag taaaagtatt attagaattt
     9481 taattaaata aaa
//
LOCUS       DAMJWP010000133         9409 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.138, whole genome shotgun sequence.
ACCESSION   DAMJWP010000133 DAMJWP010000000
VERSION     DAMJWP010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9409
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.138"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            57..1013
                     /locus_tag="PC700_002532"
     CDS             57..1013
                     /locus_tag="PC700_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HDF2581831.1"
                     /translation="MYILNLNTRETIEDFRDKFYVAENSYLILSAPKNLKLLKETLDI
                     DEITFNDCLKFDEITKLDLFDNYDFLSLNTFELRDGEAVIEEVNMYLSDNFILVVVNE
                     EHFLFEFVKNIILKNSQLEKNPVINLFKINYLILREVIKNGFESLEKVEELILQIEDE
                     MMDNINKNHVSRISDVRGLTRIIVKNTRPLLYIGDRIVKENIRYLKYSNVKKYNLENF
                     QGIDFGIDKLYNFALSTRELADKLLDIYSSRVGEKTNNLITKLTLLTAISAPLTIITG
                     IYGMNFRYMPELNWIYGYPATLFFMLCIIFIGIIIFKIKKLL"
     gene            1190..1345
                     /locus_tag="PC700_002533"
     CDS             1190..1345
                     /locus_tag="PC700_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581832.1"
                     /translation="MKGILKELTAKLYSFFYFMFWGFYFSAGYFFYKNICTSFLVRPY
                     FSDFEHK"
     gene            1475..2488
                     /gene="aroF"
                     /locus_tag="PC700_002534"
     CDS             1475..2488
                     /gene="aroF"
                     /locus_tag="PC700_002534"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDF2581833.1"
                     /translation="MIVVLKMGADKNEVKKLIEAIGREGVEVNPIDGTELTVLGLVGD
                     TSKIDAKRIEANKIVEKVMHVVEPFKKANRKFHPEPSIINVNGMEIGSKKIAMIAGPC
                     SVETEDQIVSIAKDVKKSGAGFLRGGAFKPRTSPYAFQGLKYDGLDLLKKAKEKTGLP
                     IVTEIMSTQDIDIFEENVDVIQVGARNMQNFDLLKELGKTNKTILLKRGLSATIEEWL
                     MSAEYIMAGGNENVVLCERGIRTFETYTRNTLDLSAILAVKKLSHLPVIVDPSHAAGK
                     SWMVDSLSKAAIAVGADGLIIEVHNDPAHALCDGKQSIKPNEYDELISELKTIASAVG
                     REI"
     gene            2510..3574
                     /locus_tag="PC700_002535"
     CDS             2510..3574
                     /locus_tag="PC700_002535"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDF2581834.1"
                     /translation="MEEKLLVNLKENSYNIFIKKNILKNIGEEIKKIYFGNKIFIVTD
                     ENIAKLYLDKVKNNLSESGFEIKSLILKSGEETKSFNTLPKIYDSLLDFKLTRKDLII
                     TLGGGVIGDLGGFAAATFLRGISFIQIPTSLLAQVDSSVGGKVGVDLERGKNLVGSFY
                     QPKAVFIDPNVLLSLPEKFYRDGMAEVIKYGCIKDKTFFNKLLSLKNREEVMQHIEYI
                     IHKCCFIKKTVVEADEKDFGDRMLLNFGHTLGHAIEKYYNFTGYTHGEAVAIGMYKIT
                     LESEKIGITKKGTSELIKSILMNYNLPYEIELRDNKSIVDAISLDKKNLGDTLKIILL
                     KDIGESIIYNTTSKFFQEVR"
     gene            3574..4887
                     /gene="aroA"
                     /locus_tag="PC700_002536"
     CDS             3574..4887
                     /gene="aroA"
                     /locus_tag="PC700_002536"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDF2581835.1"
                     /translation="MGSLKIYPSKLSGDVKIPPSKSMAHRAVICSSLSNGKSRISNID
                     FSDDIIATIRAMTSLGAIIEKKEDILEISGIFSKEGILNRENQLNQPKLTIDCNESGS
                     TLRFLVPISLAFDGVKRFIGRGNLGKRPLDTYYEIFDRQNIKYSYKENQLDLIISGKL
                     KPDEFRVKGNISSQFITGLLFILPTLESDSKIIITTELESKGYLDLTLSTIKDFGVEI
                     INNNYKEFIIKGNQTYKARDYKVEGDYSQGAFYLSADAIGEDISILDLKEDSLQGDSE
                     VVEILSRMGMEILREGNKIKGITNGLNSTLIDASQCPDIIPVLSVVASLSIGRTTIIN
                     AGRLRIKECDRLHAINVELSKLGANIEEKEDSLIIEGVSKLNGGVEVWSHKDHRIAMT
                     LAIASCRCDKPIILKDFECVSKSYPHFFKDFKMLGGRIDEWNMGK"
     gene            4865..5944
                     /gene="aroC"
                     /locus_tag="PC700_002537"
     CDS             4865..5944
                     /gene="aroC"
                     /locus_tag="PC700_002537"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDF2581836.1"
                     /translation="MSGIWGNNLKVSIFGESHGNAIGINIDGLPSGIELDLDKIDKEM
                     KRRAPGKNSISTSRNESDIPEILSGYFNGRTTGTPLCAIIRNSDTRSKDYGELKNLMR
                     PGHADFTGNVRYSGFNDYRGGGHFSGRITAPLVFCGAICKQILSQKGIEIGAHIKKIK
                     NIEDMSFDYVNISKQQLSNLQTLELPLLDLSKEEAMKNTIIDAKNQRDSVGGIIECAV
                     VGINVGLGNPFFDSVESTLSHLLFSVPAVKGVEFGLGFELADMYGSQSNDEMYYEGNQ
                     VKSKTNNNGGIIGGITTGMPIIFKVAIKPTPSISRQQNTVNIKDKKDDILYIKGRHDP
                     CIVQRAIPVIEAVTAIGIFDLMKGR"
     gene            5947..7143
                     /gene="pheA"
                     /locus_tag="PC700_002538"
     CDS             5947..7143
                     /gene="pheA"
                     /locus_tag="PC700_002538"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HDF2581837.1"
                     /translation="MEDLTRYRNEIDNIDKEIIQLFEKRMNTVLEIARHKMKNNTTIL
                     QKDREEKVLSRAIDNLDNKAYSQETVQFFNSIMEISRGLQKRLIDNDGEQKIEFSLDS
                     SNKKFDLSNVNKYKSLKDELNKKNILVGFPGKSGSFTEEALNKFFNKTISKKQFKEFE
                     DVFIALKNKEINYGIIPIENSSTGAISETYDLLRKYGFYIVGEECIKIDQNLIGIKDT
                     KLEDIKEIYSHPQGIGQCSEFLKQNSTWKLIPFNNTATSAELVRKLQDKTKAAIASKK
                     AASIYELEIISPCINDIANNYTRFVVISNQIHIEEESNKMSVVFSVEHEAGKLYKVLG
                     YFAENNINMTKIESRPMKNASWRYFFYIDFECSIYNSKVYDLLELIEHNTAYFKFMGV
                     YKNKTE"
     gene            7190..7999
                     /gene="aroE"
                     /locus_tag="PC700_002539"
     CDS             7190..7999
                     /gene="aroE"
                     /locus_tag="PC700_002539"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDF2581838.1"
                     /translation="MNFFGLVGEKLSHSVSPQIHKRIFEILNIESAYKNFEIPKEDIS
                     KLDGAIKLLGIQGVNVTVPYKERIMKYLDFISPEAKRIGAVNTILLRENMLYGYNTDY
                     FGLDSMFKMANIDVQGKVAVILGTGGASKAALTYFIDSGIEKLYVSTRKKDDKKLLNS
                     KAILIDYEELKHIKGDIILNATPVGMYPNVGISPVSKSIIQNFDILIDLIYNPGETEF
                     LRIGNSMGKKTCDGLYMLVGQAIKSQEIWQDTKIDNSILDVIYNELKLEFL"
     gene            8011..8544
                     /locus_tag="PC700_002540"
     CDS             8011..8544
                     /locus_tag="PC700_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HDF2581839.1"
                     /translation="MINKTKEKLILIGMPGSGKTTIGKLLAKEYNCSFCDMDDYIIQI
                     SQKSIAELFSEGEDIFRNYETQACRELSISDKTVISTGGGVIKKDVNMEILKETGIII
                     FIDRPIQKILEDININSRPLLKNGKDRLYNLYNERINLYKKFSDIEILNDKSLNNAVY
                     NITNAVSENFKFDFKEK"
     gene            8565..9404
                     /locus_tag="PC700_002541"
     CDS             8565..9404
                     /locus_tag="PC700_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HDF2581840.1"
                     /translation="MNIVIVGLGVIGGSFAKALKKAGYENVFGVDVDLETLKKAEKAK
                     IIKKGCTTGKELFKKADLIILSIYPRLVVDFLNNNKNFFKKGTIITDTTGIKETLIND
                     VLQIIPDDIDFIFGHPMAGREKKGIDFASEQVFNGANYIITPTGRNNIKNLELVENLI
                     LEIGFKRVKKLTSQKHDEIIAFTSQLPHVMAVALINSDEEGRDTGKFIGDSYRDLTRI
                     ANMNEDLWSELFLGNRDNLLKVIENFESEVNLVKEAIFNNDKNKLIEYFKKSSIRREA
                     LEK"
BASE COUNT         3876 a          923 c         1655 g         2955 t
ORIGIN      
        1 acagtaagga ttataaaaaa tttttagaat gtaaattgaa atttaggagt agatgtatgt
       61 atatactgaa tttaaataca agagagacta ttgaagattt tagagataaa ttttatgtag
      121 cagaaaattc ttatcttata ttaagtgctc caaaaaattt aaaattgctt aaggaaactt
      181 tggatataga tgaaataact tttaatgatt gtcttaaatt tgatgaaata accaaacttg
      241 atttatttga taattacgat tttttaagcc taaatacatt tgaactaaga gatggagaag
      301 ctgttataga agaagtaaat atgtatttat cagataattt tatattggtt gtagtgaatg
      361 aagaacactt tttatttgaa tttgtaaaaa atattatttt gaagaactct caacttgaaa
      421 aaaatccagt tattaatcta tttaagataa actacttgat tcttagagaa gttataaaaa
      481 atggttttga gtctttggaa aaagttgaag agttaattct tcaaatagaa gatgaaatga
      541 tggacaacat taataagaat catgtatcta gaataagtga tgtaagagga ttaacaagaa
      601 ttatagttaa gaatactaga cctcttttgt atattgggga tagaatagtt aaggaaaata
      661 taagatattt aaaatattca aatgtaaaaa aatataattt agaaaatttt caaggtatag
      721 attttggaat tgacaaatta tacaatttcg cattgtcaac acgagagtta gcagataaat
      781 tattagatat ttattcttcc agagtaggtg agaaaacaaa taacttaatt acaaaattaa
      841 ccttgttaac agccatatca gcaccactta caattataac tggtatatat ggaatgaact
      901 ttagatatat gccagaatta aactggattt atggatatcc agcaacttta ttttttatgc
      961 tatgtataat ttttattgga attataatat ttaaaataaa aaaattattg taaattacag
     1021 atgatgttta attgatttta taaaaataca tcaattgaga agctgattta agtaaataaa
     1081 aaaatatttt aaattgaaaa tatttataga aaatattgac aattatattt tctttgttat
     1141 aattattaaa caagaagaat aataaattat aaaaatggag gataatacta tgaaaggaat
     1201 attaaaagaa ctaacagcaa aactttatag ctttttttac tttatgttct ggggctttta
     1261 ttttagcgct ggctatttct tttacaaaaa tatatgtact tcctttctgg tgaggcctta
     1321 ttttagtgat tttgagcata agtaaatttt ataaaaatga ttattttata tatttatata
     1381 agatttagtg cttgatagat tattttagag aactcattag tgagttctct tttttatttt
     1441 aaatataaaa ttataatatt gggaggtagt aaaaatgata gtagtattaa aaatgggagc
     1501 agataaaaat gaagttaaaa aattaattga ggctattggg agagaaggag tagaagttaa
     1561 tcccatagat ggaacagaat taactgtttt aggtttagtt ggagatacta gtaaaataga
     1621 tgccaaaaga atagaagcaa ataagatagt tgaaaaagtt atgcatgtag tagagccttt
     1681 taaaaaggcc aatagaaaat ttcatccaga accttcaatc ataaatgtta atggaatgga
     1741 aattggttca aagaaaattg ctatgatagc tgggccttgt tctgttgaaa cagaagacca
     1801 aatagtatct atagctaaag atgtgaaaaa atcaggtgca ggatttttga gaggtggagc
     1861 attcaaacca agaacatcac catacgcttt ccaaggattg aaatatgatg gattggattt
     1921 attaaaaaaa gcaaaagaaa aaacagggct tcctatagtt acagaaataa tgtcaacaca
     1981 agatatagat atatttgaag aaaatgtaga tgtaattcaa gttggagcta gaaacatgca
     2041 gaactttgat ttattaaaag aattaggtaa aacaaataaa acaatacttt taaaaagagg
     2101 attatctgct acaattgaag agtggctaat gtcagcagag tatataatgg ctggaggaaa
     2161 tgaaaatgta gttctttgtg aaagaggtat aagaactttt gaaacatata ctagaaatac
     2221 tttggattta agtgcaattt tagctgtgaa gaaacttagt catttaccag ttatagttga
     2281 cccaagtcat gcagcaggta aatcatggat ggttgactct ttatctaagg cagcaattgc
     2341 agttggagca gatggtctaa taatagaagt tcataatgac ccagcacatg cactttgtga
     2401 tggaaaacaa tcgattaagc caaatgaata tgatgaattg ataagtgaat taaagactat
     2461 cgcatcagca gttggtagag aaatataatc tataaaagga tggttaaacg tggaagaaaa
     2521 actattagta aatttaaaag aaaatagcta caatatattt attaaaaaaa atatactaaa
     2581 gaatatagga gaagaaataa aaaaaatata ttttggaaat aagattttta ttgtaactga
     2641 tgaaaatata gcaaaattat acttggataa agtaaaaaat aatttaagtg aatctggatt
     2701 tgaaattaaa tctttaatat taaaatctgg tgaagaaaca aaatcattta atactcttcc
     2761 taaaatatac gatagtttac ttgactttaa gttaactaga aaagatttga ttattacttt
     2821 aggaggaggg gtaataggtg atttaggtgg atttgcagca gcaacttttt taagaggtat
     2881 aagttttatt caaattccaa catctttatt agcacaagtt gatagttctg taggtggaaa
     2941 agttggagtt gaccttgaga gaggcaaaaa tttagtggga agcttttacc aaccaaaagc
     3001 tgtgtttatc gacccaaatg tattattatc acttccagaa aaattctata gagatggaat
     3061 ggcagaagtt ataaaatatg gatgtataaa agacaaaact tttttcaata aacttttaag
     3121 tttaaaaaat agagaagaag taatgcaaca tatagaatac attatacata aatgttgctt
     3181 tattaaaaaa actgtagttg aagctgatga aaaagacttt ggagatagaa tgcttctaaa
     3241 ctttggccat acattaggtc atgctattga gaaatactac aattttacgg gttataccca
     3301 tggagaagct gttgccatag gaatgtataa gataacttta gaaagtgaaa agataggaat
     3361 tacaaaaaaa ggtacatcag aacttataaa atcaatttta atgaattata atttgcctta
     3421 tgagattgaa ttaagagata ataaatctat agtagatgct atatctcttg ataaaaaaaa
     3481 tctaggtgat acattaaaaa taatactttt aaaagatatt ggagagagta ttatatataa
     3541 tactacttct aaattttttc aggaggtaag ataatgggaa gtctaaaaat atatccaagt
     3601 aaattatctg gagatgtaaa aataccacca tcaaaaagta tggcacatag ggcagttatt
     3661 tgttcatctc taagcaatgg aaaaagtaga atttctaata tagatttttc tgatgatata
     3721 atagctacta taagagcaat gacatcatta ggagctatta tagaaaagaa agaagatatt
     3781 ttagaaattt cgggaatttt ttctaaagaa ggtattttaa atagagagaa tcagttgaat
     3841 caacctaaat tgacaataga ttgtaatgaa tcaggctcta cattaagatt tttagtacct
     3901 atatctttag catttgatgg tgtaaaaagg tttataggta gaggtaatct tggaaaaaga
     3961 ccacttgata cttattatga gatatttgac agacagaata taaaatatag ttacaaagaa
     4021 aatcagttag atttgattat atcaggtaag cttaaaccag atgaatttag agtaaaagga
     4081 aatataagtt ctcagtttat cacaggtcta ttgtttatat tgcctacact agagagtgat
     4141 tcaaagataa taattactac tgagctagaa tctaaaggat atttagattt aactctttca
     4201 acgataaaag attttggggt agaaattata aacaataact ataaagaatt tattataaaa
     4261 ggaaatcaaa cgtataaagc gagagattat aaagtagaag gagattattc tcaaggagca
     4321 ttttatttat cagcagatgc tataggagaa gatattagca tattagacct taaagaagat
     4381 tctcttcaag gggatagtga agtagttgaa attttatcta ggatgggaat ggaaatatta
     4441 agagaaggca acaaaataaa aggaataaca aatggtttaa atagtacttt gatagatgca
     4501 tctcaatgcc cagatataat acctgtatta tctgtagtag catccttaag tatagggagg
     4561 actacaataa ttaatgctgg taggcttaga attaaagaat gtgatagact tcatgcaata
     4621 aatgttgagt taagtaaact tggagcaaat atagaggaga aagaggatag tttgataatc
     4681 gaaggagtaa gcaagttaaa tggtggagta gaggtgtgga gtcacaaaga ccatagaatt
     4741 gctatgacac ttgcaattgc ttcttgtaga tgtgataaac caataatatt aaaagatttt
     4801 gagtgtgtat caaaatctta tccacatttt tttaaagatt ttaagatgtt aggaggtcga
     4861 attgatgagt ggaatatggg gaaataattt aaaagtatca atttttggag aatctcatgg
     4921 aaatgctata ggtataaata tagatggtct tccttcgggg attgaacttg accttgataa
     4981 gattgataaa gaaatgaaga gaagagcacc tggaaaaaat tctatatcta cttcaagaaa
     5041 tgaaagtgat ataccagaaa ttctaagtgg atattttaat ggaagaacta caggaacgcc
     5101 attatgtgca atcataagaa atagtgatac tcgttcaaaa gattatggag agcttaaaaa
     5161 tttaatgaga ccaggacatg cagatttcac aggtaatgtg agatattctg gatttaatga
     5221 ctatagaggg ggaggtcatt tttcaggtag gataacagct ccattagtat tttgtggggc
     5281 tatatgtaaa caaattttat cacaaaaagg aatagagata ggagcacaca taaaaaaaat
     5341 taaaaatata gaagatatga gttttgatta tgtaaatatt tcaaaacaac agttatcaaa
     5401 tttgcaaact ttggaactac ctttacttga tttatcaaag gaagaagcca tgaaaaatac
     5461 gattatagat gcaaaaaatc aaagagattc agttggtggt ataattgagt gtgcagtagt
     5521 aggtattaat gtagggcttg gaaatccatt ttttgactct gttgaatcta ctttatcaca
     5581 tttattgttt tctgttcctg ccgtaaaagg tgtagagttt ggtttaggtt ttgaacttgc
     5641 agatatgtat ggctctcaaa gtaatgatga aatgtattat gaaggaaatc aagttaagag
     5701 caaaacaaat aacaatggag ggattatagg agggattact actggaatgc caataatttt
     5761 caaagtggca attaagccaa ctccttcaat tagcagacaa caaaacacag ttaatataaa
     5821 agataaaaag gacgatattt tatatattaa aggcagacat gacccttgta tagttcaaag
     5881 ggctatacct gtaatagaag cagtaacagc cattggaatt tttgatttga tgaaaggacg
     5941 ataataatgg aggatttaac gagatataga aatgagattg acaatataga taaagagatt
     6001 attcaattat ttgaaaagag aatgaataca gttcttgaaa ttgccagaca taaaatgaaa
     6061 aataacacta ctatacttca aaaagataga gaagaaaaag tactaagtag agccatagat
     6121 aatttagata ataaagcata ctcacaagaa acggtacagt tttttaattc tataatggaa
     6181 attagtagag gattacaaaa aagattgata gataatgatg gtgagcagaa aatcgagttt
     6241 agtttagatt catcaaataa gaaatttgat ttgtcaaatg taaataaata taagtcttta
     6301 aaagatgaat taaataaaaa aaatatccta gtaggatttc caggaaaatc aggttcattt
     6361 acagaagaag cactaaataa attcttcaac aaaacaatat caaaaaaaca atttaaagaa
     6421 tttgaagatg ttttcatagc acttaaaaat aaagaaataa actatggaat aatccccata
     6481 gaaaattcat ctacaggagc tatctctgaa acttatgacc ttttgagaaa gtacggtttt
     6541 tatatagttg gagaagaatg tataaaaata gaccaaaatt taatagggat aaaagacaca
     6601 aaacttgaag atataaaaga aatatattct catccacaag gtataggaca atgtagtgaa
     6661 tttttaaaac aaaatagtac atggaaattg ataccattta ataacacagc aacaagtgca
     6721 gaacttgtaa gaaaacttca agataaaaca aaagcagcaa ttgcaagcaa aaaagctgca
     6781 agtatttatg aattagaaat aataagtcct tgtataaatg atattgcaaa caactataca
     6841 agatttgtag taatcagtaa tcaaatacac attgaagaag aatcaaacaa aatgagtgta
     6901 gtattttctg ttgaacatga agcaggaaaa ctttataaag tacttggata ctttgctgaa
     6961 aataatataa atatgacaaa aatagagtca agacctatga aaaatgcctc atggagatat
     7021 ttcttttata tagattttga gtgttctata tacaattcta aagtgtacga cttattagaa
     7081 ttgatagaac ataatacagc atattttaaa tttatgggag tatataaaaa taaaactgaa
     7141 tagataatat aaatttttat ttaatttata attattagga ggtgtaagta tgaatttttt
     7201 cggtctagta ggtgaaaaac tctctcacag tgtttcacct caaatccaca agagaatatt
     7261 cgagatattg aatattgaaa gtgcttataa aaactttgaa attccaaaag aagatatatc
     7321 taagctagac ggtgctataa aattattagg aatacaagga gtaaatgtta ctgtaccata
     7381 taaggagcgt attatgaaat atcttgattt tatatcacca gaggcaaaac gtataggagc
     7441 agttaataca attttactca gagaaaatat gttatatggt tacaatacag attactttgg
     7501 tcttgatagt atgtttaaaa tggctaatat tgatgttcaa ggaaaagtag cagttatatt
     7561 aggaactgga ggtgcttcta aagctgctct tacatatttt attgattctg gtatagaaaa
     7621 gctttatgtt tcaactagaa aaaaggatga taaaaaatta ttaaatagta aagctatatt
     7681 aatagattat gaagaactta aacatataaa aggagatata atattaaatg ctacaccagt
     7741 aggtatgtac cctaatgtag gaatttcacc agtgtcaaaa tctattatac aaaattttga
     7801 tatattaata gatttaattt acaatcctgg agaaacagag tttttaagaa ttggtaatag
     7861 tatgggtaaa aaaacttgtg atggattata tatgctggta gggcaagcaa taaaatctca
     7921 ggaaatttgg caagatacta aaatagataa tagtatttta gatgttatat ataatgaact
     7981 aaaattagaa tttttgtaag ggagattgtc atgataaata aaacaaaaga aaaattgata
     8041 ttaataggta tgccaggtag tggaaaaact acaattggta aacttttggc caaagaatat
     8101 aattgtagtt tctgtgatat ggatgattat ataattcaaa tatctcaaaa aagtatagca
     8161 gagttgtttt cagaaggaga agatatcttt aggaactatg agactcaagc atgtagagaa
     8221 ctttctataa gtgataaaac agtgatatct acaggtggag gtgtaataaa aaaggatgtt
     8281 aatatggaaa tactaaaaga aacaggtata ataattttta ttgatagacc tatacaaaag
     8341 attttggaag atataaatat taattcaaga ccattattaa aaaacggaaa agatagattg
     8401 tataatcttt ataatgagag aataaatctt tataaaaaat tttcagatat agagatttta
     8461 aatgataaat cattaaataa tgctgtatat aatattacaa atgcagtcag tgaaaatttt
     8521 aaatttgact ttaaagaaaa ataatctagg gggaaataaa aataatgaat attgttattg
     8581 taggactagg agttatagga ggctcttttg ctaaagcatt aaaaaaagct ggatatgaaa
     8641 atgtatttgg agtagatgta gatttagaaa ctttaaaaaa agcagaaaaa gcaaaaatca
     8701 taaaaaaagg atgtactaca ggtaaagagt tatttaaaaa agctgattta attatactat
     8761 ctatatatcc tagattagtt gtagattttc taaataataa taaaaacttt tttaaaaaag
     8821 ggactataat tactgatact acaggaataa aagaaacact tatcaatgat gtgttgcaaa
     8881 ttattccaga tgatatagat tttatatttg gacatccaat ggcaggtagg gaaaaaaagg
     8941 gtattgattt tgcaagtgaa caagttttta atggcgcaaa ttatataatt actccaactg
     9001 gcagaaacaa cataaaaaat ttagagttag ttgaaaattt aattttagag attggattta
     9061 aaagagtaaa aaagttaact tcacaaaaac atgatgagat tatagctttt acttctcagt
     9121 tacctcatgt tatggcagtt gctcttataa atagtgatga agaaggtaga gatacaggta
     9181 aatttattgg agatagttat agagatttaa ctagaattgc aaatatgaat gaagacttat
     9241 ggagtgaact ttttttagga aatagagata atttactaaa agttatagaa aactttgaat
     9301 cagaagtaaa tttagtaaaa gaagctatat ttaacaatga taaaaataaa ttgatagagt
     9361 attttaaaaa atcatcaata agaagagaag ctttagaaaa ataaatatt
//
LOCUS       DAMJWP010000134         9405 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.139, whole genome shotgun sequence.
ACCESSION   DAMJWP010000134 DAMJWP010000000
VERSION     DAMJWP010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9405
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.139"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(122..775)
                     /locus_tag="PC700_002542"
     CDS             complement(122..775)
                     /locus_tag="PC700_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2581841.1"
                     /translation="MKVDPLTTQVYDYISKKIQNGEYEANQRITESEICKSIGVSRTP
                     AREALTRLAGENLLEKIPNKGFVVKEFQEKEKLDTYSVIGVLDALAGSSALQNLTESD
                     LVKMEELTEMMAVSIKYKNYNSYLKLSNEFHDIYITKSDNQVLVNLLNSLRYNFMSKS
                     YTSDNEDELYKMLTYSNNQHIEVVKFMRENDLENVERVLREHWKTIPIAEMKKEKLL"
     gene            complement(1020..2885)
                     /locus_tag="PC700_002543"
     CDS             complement(1020..2885)
                     /locus_tag="PC700_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase C-terminal domain-containing
                     protein"
                     /protein_id="HDF2581842.1"
                     /translation="MQSVIEYNSWDKNFKAPFGALKFDEELTICVKVNEGYNIKSISL
                     EINREEEMRTITLNEELDNDKLGKCFCGKIEKFDGTGVYFYYFKVDVEMDGQIKTLFY
                     GKNRDNGYSCEYNYSDINKYQITVYKDFKVPTWYKEGILYHIFVDRFNNGNRSGKVDN
                     PKKNSFIYGNWEDIPMYIKDSQGDVIRWDFHGGNLRGIINKLGYLKKLGVSILYLSPI
                     FEASSNHKYDTGDYKKIDPMFGDEDTFKELIDKAKEKGISIVLDGVFSHTGADSKYFN
                     MYGNYNSLGAYQSKESPYYSWYMFEEFPQKYKSWWDVKTLPNINELEHSYMDYIIYDN
                     DSVINKWVNMGIKGWRLDVADELPTKFIRELKKELKKADDDSILIGEVWEDASNKISY
                     GQRRSYLLGEELDSVMGYPFRNNMFSFLKGEINSYELCNRYMQIKENYPKESFRSNLN
                     LIGTHDVTRAKTELNDDVDLVKLAVAIQMTFEGVPYIYYGDEAGLCGGVDPDNRRTYP
                     WKNEDEDMLNFYRDVIKIRNKNKLLSSGNTEFIYTNNDSVFSFIRVDENNDRMLILVN
                     RSENVESISLCIESSFIEEIPIKYSLKNANSTIQIENNELKVDLDSKSFRIFRVN"
     gene            complement(2890..5310)
                     /locus_tag="PC700_002544"
     CDS             complement(2890..5310)
                     /locus_tag="PC700_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HDF2581843.1"
                     /translation="MVTISKKKFKQKFEVKMYSLYAQSIKEATDEQLLNVLCSLLKDE
                     IAKKWVATKLDKKKEVYYFSLEFLIGRQLKSNLLNLNIEEEVREGLSELGINLDDLIE
                     AEVDPALGNGGLGRLAACFLDSMASLNISGQGYGIRYKYGLFEQKFVNGYQVEVPDNW
                     LTEGRYAWETVRPNEATMVKFGGEVELIKEGSHLKVIHKNYLPVMAMPYDIPIIGYQN
                     QCINTLRLFKSEIPKRDFGELTSNALNYSGSYEEALKHKYYTEEISQVLYPDDSNYAG
                     KLLRLKQEYFFVSAGIQDIIRKYKKNKLNIDNLFDKVAIHINDTHPTLCIPELMRILL
                     DEENLSWDEAWEITKKTVSYTNHTIMSEAMEKWPVSMMKELLPRIYMIIEEINRRYVE
                     ELNNKGYEQDRIKRMSIIDCDNINMANLCIVTSHSVNGVAKLHTHILETEVLKDFYQD
                     EPNKFNNKTNGIAHRRWLISSNPQLSNLITDLIGDSWKKDTLQLKNIEKFKNDSSVLQ
                     RLDDIKYNNKANLAKFIKDKYDLNVDPSSIFDVQVKRLHAYKRQLLNIFNVLHMYHEL
                     LDNPNLNLDPRTFIFGAKAAPGYYLAKCIIKFINSVASTINNDVRVKDKLKVVFLENY
                     GVSLAEIIIPAANVSEQISTTTKEASGTSNMKFMMNGAITLATLDGANVEICEQVGKE
                     NMFLFGLSAEQVLNYNKYGGYSSLDLYHSNMDIKRVVDDLINGFIPNLGEEGRSIYNS
                     LTTYNDEYFVLRDFENYGQAQADINRLYRDKEKWNKMSLVNIANSGFFSSDRTISEYA
                     KDIWFKRV"
     gene            complement(5324..6766)
                     /gene="glgA"
                     /locus_tag="PC700_002545"
     CDS             complement(5324..6766)
                     /gene="glgA"
                     /locus_tag="PC700_002545"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011053260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HDF2581844.1"
                     /translation="MKVFYVTAECWPFAKTGGLGDVSYALPKELKKEGVDVRVIMPKY
                     STIPSYLKDQLKEIAVFSVRVGWRNQYCGLLEMELDGVKFYFIDNEFYFRREDERKSI
                     YGYGDDAERYTFFTDAVLEAISRIDFYPDVIHINDWHTGMLPLILKERYATLEGYKNI
                     KTMYTIHNLQYQGVFDKHVLYDILDLPQKYFDNRDIEYYGSINFMKAGINFADKIITV
                     SPTYANEIQTSFYGEQLDGLLRKESGKLKGILNGIDYDLNDPAKDKDIFVHYDVDSID
                     KKVENKLRLQDILGLKKDSSIPLIGIVSRLVSQKGFDLIAYMMPELMREDLQIVVLGT
                     GEHQYQSMFNYYDSNFSDKVSARITFNASLAQQIYAASDMFLMPSLFEPCGIGQMLAM
                     RYGSLPIVRETGGLRDTVTPYNKFTGEGNGFSFKNYNAHEMFFCLKNAIKVFKDKEKW
                     IKLVENAMKTDNSWKKSAKEYIETYRDICD"
     gene            complement(6795..7925)
                     /gene="glgD"
                     /locus_tag="PC700_002546"
     CDS             complement(6795..7925)
                     /gene="glgD"
                     /locus_tag="PC700_002546"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HDF2581845.1"
                     /translation="MRNECLGIINLNKKGDPAINKLNYGRPIASTPIAGRYRIIDFAL
                     SNMINSGITKVGIFAKEKYRSLTDHIGSGKDWDLSRKKGGLSIFSPENTKYRNTYSHR
                     EGDIYTILANLDYIEKSEEEYILIAPSYMLCNLNYSQALEYHKKSHNDITIIYKNVNN
                     ANKDFAGNLTLNLDSNNRVINVGNNLGKFPRANICMETYIMKREDFVECIYNIVNKGS
                     YCYLEEFIIEEAENMKIGAYEYTGYLKCVNSVESYFEMSKDLLNLEVADELLYSERKI
                     FTKEKNESPTIYTDSAKVENSFIASGCLIEGTVKDSIIFRKVNVEKGTVIENSIVMQN
                     CVIKSNAKLYNAILDKNTSVSNGKELRGDEKYPLVVEKNTNI"
     gene            complement(7927..9087)
                     /locus_tag="PC700_002547"
     CDS             complement(7927..9087)
                     /locus_tag="PC700_002547"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HDF2581846.1"
                     /translation="MKKEMLAMILAGGQGSRLGVFTKRIAKPAVSFGGKYRIIDFVLS
                     NCSNSGIDTVGVLTQYRPLILNSHIGMGSHWDLDRINGGVYVLQPFMNEKEGNWYNGT
                     AHAIYQNMDFVDTYNPEYVLILSGDHIYKMDYSKMLKFHKEKRSKATIAVIEVPWDEA
                     SRFGIMNTNEDSSIYEFEEKPSEPKSNLASMGVYIFDWKMLRNYFKEAEKNPEINYDD
                     FGKNLIPKMLEDNVGMYAYPFKGYWRDVGTIQSLWDANMDIIKSPETLDLADPKWKIY
                     TNTMAMPPQYIGKNANVHRSMIADGCRILGEVGNSVLSHGVVVGKGSKVIDSVIMPNV
                     VIGENVTIEKAMIGECATINDNVQIKNVNNEINVVSEYENIEPRCVLIEGGL"
BASE COUNT         2972 a         1726 c          914 g         3793 t
ORIGIN      
        1 tatattattc tatcgtagtg ccttagagtt tttatttgta tttaaagtta aatttattct
       61 tgtttttcct ttaatattta atagtaaaat aacaaaatta taaaaaaaat aaagagaaac
      121 tttataaaag cttctctttt ttcatctctg ctataggtat cgttttccaa tgttctctca
      181 aaactctttc aacattttct aaatcattct ccctcataaa ttttactact tcaatatgtt
      241 gattattgct gtatgtcaac attttatata actcatcttc attatcacta gtatatgact
      301 ttgacataaa attatatctt aaagagttca atagatttac taacacctga ttatctgatt
      361 tagttatgta tatatcgtga aattcattac ttaattttaa atagctattg taatttttat
      421 actttataga gaccgccatc atttctgtta attcttccat ttttactagg tctgattcag
      481 ttaaattttg taatgctgaa gaaccggcta acgcatccaa tactccaatt acggaatagg
      541 tgtctaattt ctccttttcc tgaaattctt ttactacaaa tcccttatta ggtatttttt
      601 ctagtaaatt ttcacctgct aacctagtta atgcttctct agctggtgtt ctactaactc
      661 cgatagattt acaaatctca gattcagtaa ttctctgatt agcttcatac tctccatttt
      721 ggattttctt tgatatatag tcatatactt gagttgtaag tggatctacc ttcataagac
      781 gttcctccat attaagatta taaaagttaa atctccctac aactttggta gagagttcag
      841 ttttatctta ctaatttata taatatcaca attatgatat tttttatact tctttgtaat
      901 attttgaaac actaaaattc ttatgataat ttatacaaaa aaagtagaaa aagttttgat
      961 aaaacaaaac tctttctaca ttcaccttat tttctattta tttttctaaa aaaagatatt
     1021 taatttactc taaaaattct aaatgattta gagtctaaat ctactttcaa ctcattattt
     1081 tcaatttgta tagtactatt tgcattttta agactatact ttataggtat ttcctcaata
     1141 aaactactct ctatacataa tgatatactt tccacatttt cacttctatt tactaaaatt
     1201 aacattctat cattattttc atcaactcta ataaaagaaa atacagaatc attattagta
     1261 tatataaatt ctgtatttcc agaacttaaa agtttattct tatttctaat ttttataaca
     1321 tctctataaa aattaagcat atcttcgtct tcatttttcc atggataagt tcttctatta
     1381 tctgggtcaa cacctccaca aagacctgct tcatctccat aatatatata tggtactcct
     1441 tcaaatgtca tctgtatagc tacagcaagc ttcactaaat ctacatcatc atttaattcc
     1501 gttttagctc ttgtaacatc atgtgttcca attaaattta aattagacct aaatgattct
     1561 tttggataat tttctttaat ctgcatatat ctattacaaa gttcatatga attaatttct
     1621 ccttttaaga aagaaaacat gttatttcta aaaggataac ccataacaga atctaattct
     1681 tcacctaata aataacttct tctctgacca tagctaatct tatttgatgc atcttcccat
     1741 acttcaccta taagaattga gtcatcatct gcctttttta attccttttt taactctctt
     1801 ataaacttag ttggtaattc gtctgcaaca tctagtctcc agcccttaat acccatattt
     1861 acccatttat ttataacgct atcattgtca tatattatat aatccatata gctatgttct
     1921 aattcattta tatttggcaa tgtcttaaca tcccaccaac ttttatactt ttgaggaaat
     1981 tcttcaaaca tataccatga ataatatgga gattctttag attgatatgc ccctaaacta
     2041 ttatagtttc catacatatt aaaatattta ctatctgctc cagtatgact aaaaactcca
     2101 tctaatacta ttgatatacc tttttctttt gctttatcaa taagttcttt aaatgtatct
     2161 tcatccccaa acattgggtc aattttttta tagtcacctg tgtcatattt atgattacta
     2221 gaagcttcaa atatagggct tagatatagt atacttacac ctaatttttt taaatatcca
     2281 agtttattga ttatccctct taaatttcca ccatggaaat cccatcttat aacatcccct
     2341 tgactatcct ttatgtacat aggtatatct tcccaatttc catatatgaa actatttttt
     2401 ttaggattat caaccttacc actacgattt ccattattga atctatctac aaatatatga
     2461 taaagtattc cttctttgta ccatgttggt actttaaaat ctttgtaaac agtgatttga
     2521 tatttattaa tatcactata attatattca caagaatatc cattatctct gttttttcca
     2581 taaaataaag tctttatctg accatccatt tcaacatcaa ctttaaagta ataaaaataa
     2641 acacctgtac catcaaactt ttcaatctta ccacaaaagc actttccaag tttatcatta
     2701 tctaattctt catttaaagt tattgttctc atttcttctt ctctatttat ttctagggaa
     2761 atagacttga tattgtatcc ctcatttact ttaacacaaa tggtcaattc ctcatcaaat
     2821 tttaaagctc caaacggagc cttaaaattt ttatcccaag agttatattc tattacactt
     2881 tgcatttaat cacacccttt taaaccaaat gtcttttgca tactcagaaa ttgttctatc
     2941 agaagaaaaa aatccagagt tagctatatt aactaaagac attttattcc atttttcttt
     3001 atctctatat aacctgttta tatctgcttg agcttgacca taattttcaa aatctctcaa
     3061 tacaaaatac tcatcattgt aagtagtgag tgaattataa atacttcttc cttcttcgcc
     3121 aagattagga ataaatccat ttattaaatc atctaccacc ctttttatat ccatattaga
     3181 atgatataag tcaagtgaag aatatccacc atatttatta taatttagta cttgttctgc
     3241 actaagtcca aataaaaaca tattttcttt accaacctgt tcacatatct ctacatttgc
     3301 gccgtcaagt gtagccaaag ttatagcacc attcatcata aacttcatat tacttgttcc
     3361 agatgcttcc ttagttgtag ttgaaatttg ctcactaaca tttgctgctg gtattattat
     3421 ttctgctaat gatactccat aattctctaa aaatactact tttaacttgt cctttactct
     3481 aacatcatta tttatagtac ttgcaacaga atttataaat ttaattatac atttagccaa
     3541 ataatatcct ggtgctgctt ttgcaccaaa tataaaagtt cttgggtcaa gattcaaatt
     3601 aggattatca agtaattcat gatacatatg aagtacatta aatatattca gtaactgtct
     3661 tttatacgca tgcaatctct taacttgaac atcaaatatc gaacttgggt ctacatttaa
     3721 atcgtattta tcttttataa actttgctag atttgcttta ttattgtatt ttatatcatc
     3781 aagtctttgt aaaacagagg aatcattttt aaatttttca atatttttta attgtaaggt
     3841 atccttcttc catgaatcac caattaaatc tgtaattaaa ttgctaagtt gaggatttga
     3901 acttataagc catcgcctat gtgcaattcc attagtctta ttattaaatt tatttggttc
     3961 atcttgatag aaatctttta atacctctgt ttcaagaata tgagtatgaa gcttagcaac
     4021 tccatttaca ctatgacttg tgacaataca taagtttgcc atattgatat tgtcacagtc
     4081 tattatactc attcttttta ttctatcctg ctcatatcct ttattattta attcctctac
     4141 atatcttcta tttatttctt ctattatcat ataaattctt ggtaatagtt ctttcatcat
     4201 acttacaggc catttttcca ttgcttctga cattatagta tgattagtat aggacactgt
     4261 ttttttagtt atttcccatg cttcatccca agataaattt tcttcatcaa gcaaaattct
     4321 cataagttct ggtatgcata aggtaggatg tgtatcattt atatgaattg ctaccttatc
     4381 gaataaatta tcaatattta atttgttctt tttatatttt cttattatat cttgtatccc
     4441 tgcacttaca aaaaagtatt cttgtttcaa cctcaacaac ttacctgcat agtttgaatc
     4501 atcaggatat aatacctgtg atatttcctc agtataatac ttatgtttta aagcctcttc
     4561 atagctacca gaataattta gagcatttga ggtaagttca ccaaaatctc tctttggtat
     4621 ttcactctta aacaatctta aagtatttat acattggttt tggtatccga ttattggtat
     4681 atcataaggc attgccatta caggaaggta gtttttatga attactttta aatgactacc
     4741 ttccttgatt aattctacct caccaccaaa cttcaccatt gttgcttcat ttggtcttac
     4801 agtttcccat gcatatcgtc cttctgtaag ccagttatca ggcacttcta cctgataacc
     4861 atttacaaat ttttgttcaa acagaccata tttatacctt attccatatc cttgaccact
     4921 tatgttcaaa gatgccatag aatctaaaaa gcaagctgct aatctaccaa gtccaccatt
     4981 tcctaaggct gggtctactt cagcttcaat taaatcatcc aagttgattc ctaattcaga
     5041 taaaccttct ctaacttctt cttcaatatt taaatttaat aaattagatt ttaactgtct
     5101 ccctattaaa aattctaaac taaaataata aacttctttt ttcttatcta actttgtagc
     5161 aacccatttt tttgctatct catctttcaa tagactacat aatacattta acaattgttc
     5221 atcagtagct tccttgatag attgtgcata taagctatac atttttactt caaatttttg
     5281 cttgaatttc tttttgctta ttgttaccaa aaaaaccact cctttaatca catatgtctc
     5341 tatatgtttc tatatactcc ttagctgatt ttttccaact attatctgtt ttcattgcat
     5401 tttcaactaa tttaatccat ttttctttgt ccttaaatac tttgattgca tttttcaaac
     5461 agaaaaacat ctcatgagca ttgtaatttt taaaactaaa tccattgcct tctccagtaa
     5521 atttattata aggtgttact gtatctctaa gccctccagt ctctctaact attggtaaag
     5581 aaccatatct catagctaac atttgaccta taccacaagg ctcaaataat gatggcatta
     5641 agaacatatc acttgcagca tatatctgtt gagccaatga tgcattaaaa gtaattctag
     5701 ctgatacttt atctgaaaaa ttagaatcgt aatagttgaa cattgattga tattgatgtt
     5761 ctccagttcc taaaacgaca atctgtaaat cttctctcat aagttctggc atcatgtatg
     5821 ctattaaatc aaatcctttt tgagaaacta atctcgaaac tatacctatc aacggaatag
     5881 atgaatcttt tttaagtccc aatatatctt gtaatctcaa tttattttct acttttttgt
     5941 caattgagtc tacatcataa tgaacaaata tatctttatc ttttgcaggg tcgtttaaat
     6001 cataatctat accatttaat atacctttta attttcctga ctcttttcta agtaatccat
     6061 ctaattgttc accataaaaa gatgtttgta tttcatttgc ataagtagga ctaacagtta
     6121 taattttatc tgcaaaattt ataccagctt tcataaaatt tattgagcca taatactcta
     6181 tatctctatt atcaaagtat ttttgtggta agtccaatat atcatataaa acatgcttat
     6241 caaatacacc ttgatattgg agattatgta tagtatacat agtttttata tttttatacc
     6301 cctcaagggt tgcatatctt tcttttagaa ttaatggtaa catccctgta tgccaatcat
     6361 ttatatgtat tacatctgga taaaaatcaa ttctacttat agcttctaaa actgcatctg
     6421 taaagaaagt atatctttct gcatcatctc catatccata gatactcttt ctttcatctt
     6481 ctcttctaaa ataaaattca ttatctataa agtaaaattt tacaccatcc aattccattt
     6541 ctaaaagacc acagtattga tttctccatc caacacgtac actaaacaca gctatttctt
     6601 ttagttgatc ttttaaataa cttggtatag ttgaatattt gggcattatt acccttacat
     6661 ctacaccttc tttttttaat tctttaggca atgcataaga aacatctcct aatcctccag
     6721 tttttgcaaa gggccaacac tctgctgtta cataaaaaac cttcattagt cattccccct
     6781 tctaaaaaat ttatttatat atttgtattc ttttccacta caagaggata tttttcatct
     6841 cctcttaatt ctttcccatt tgatacactc gtatttttat ctaatattgc attatatagt
     6901 tttgcatttg actttattac acaattttgc attactattg agttctctat aactgttcct
     6961 ttttcaacat ttacctttct aaatatgata ctatctttta cagtaccttc tattaaacaa
     7021 ccagatgcta taaagctatt ttctacttta gcactatctg tataaattgt aggtgattca
     7081 ttcttttctt ttgtaaatat ctttctttca gagtataata attcatctgc aacttctaaa
     7141 tttaataaat ctttgctcat ttcaaaatat gattcaacag aatttacaca ttttagatat
     7201 cctgtatatt cataagctcc tattttcata ttttctgctt cttcaataat aaattcttct
     7261 aaataacaat aactgccttt atttacaatg ttatatatac attcaacaaa atcttcacgc
     7321 ttcataatat atgtctccat acatatgttt gctctaggga atttgcctaa attatttcct
     7381 acatttatta ccctattgtt actatctaaa tttagtgtta agttaccagc aaaatcttta
     7441 tttgcattat ttacattttt atatattata gttatatcat tatgagattt tttatggtat
     7501 tccaatgctt gagaataatt taaattgcat agcatatagc ttggagctat taaaatatat
     7561 tcttcttcac tcttttctat ataatctaga ttagctaaga tagtatatat atcaccttct
     7621 ctatgtgagt aagtatttct atacttagtg ttttctggac taaaaattga taggccacct
     7681 ttttttctac ttaaatccca atctttacca ctacctatat ggtctgtcaa tgatctgtat
     7741 ttttcttttg cgaatattcc aactttagtg attcctgaat ttatcatatt agataaagca
     7801 aagtctatta ttctatatct tccagctatt ggtgtagatg ctattggtct tccatagtta
     7861 agtttgttta ttgctgggtc tccttttttg tttaaattta ttatccctaa acattcattt
     7921 ctcatcttaa agacctcctt ctattaatac gcatctaggt tcgatatttt catactcaga
     7981 aactacattt atttcattat ttacattctt tatctgaaca ttatcattta tagttgcaca
     8041 ctcacctatc atagcttttt ctatagttac attttcacct attacaacat ttggcattat
     8101 tactgaatca attactttac ttcctttacc aacaacaact ccatgtgata atacagagtt
     8161 gcctacttca cctaaaattc tacaaccatc agctatcatt gatctatgaa catttgcatt
     8221 tttacctata tattgtggag gcattgccat agtatttgta tatatcttcc atttagggtc
     8281 tgctaaatca agtgtttctg gagattttat tatatccata ttagcatccc atagactttg
     8341 tatagttcct acatctctcc agtatccttt aaatggataa gcatacattc caacattatc
     8401 ttctaacatt ttaggtatta aattttttcc aaaatcatca taatttattt ctggattttt
     8461 ttctgcttcc ttaaaataat ttctcaacat tttccaatca aatatgtata ctcccataga
     8521 tgccaaattg ctcttaggtt cacttggttt ttcttcaaat tcatagatac ttgaatcttc
     8581 atttgtattc attatcccaa atcttgaagc ttcatcccaa ggaacttcaa tcactgctat
     8641 tgtagcttta gagcgttttt ccttatgaaa ttttaacatt ttgctataat ccatcttgta
     8701 tatatggtca ccagataata tcaatacata ttctggatta taggtatcta caaagtccat
     8761 attttgatat atcgcatgtg ctgtaccatt ataccaattt ccttcttttt cattcataaa
     8821 tggttgtaaa acatagacac caccatttat tctgtccaaa tcccaatgac ttcccatgcc
     8881 tatatgtgaa tttaatatca acggtctata ttgagttaaa acacctacag tatcaatacc
     8941 agagtttgaa caattactta acacaaaatc tataattcta tacttacctc caaatgatac
     9001 agctggtttg gcaattcttt ttgtaaaaac acctaatctt gaaccttgcc ctcctgccaa
     9061 aatcatagct aacatctctt ttttcattta atgacactcc cctcatctag taattaatat
     9121 ttataattgc atttttactt ttataaactt tatcagtcaa taaatgccgt gtataaatgt
     9181 tgcttaaata ataccttaat aaaaataaaa taatatcaac tatacaattg atataatgta
     9241 taccccataa tgaacttacc gacgtataca ttattttcca taagcactat agtatttatt
     9301 aatattcaat tatcggttaa aaaataatac aaattttatt ttataaatat aaaaacattt
     9361 tttataaatc ccttgtgtca tataaacatg cttatttttt aattt
//
LOCUS       DAMJWP010000135         9387 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.140, whole genome shotgun sequence.
ACCESSION   DAMJWP010000135 DAMJWP010000000
VERSION     DAMJWP010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9387
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.140"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            915..1304
                     /locus_tag="PC700_002548"
     CDS             915..1304
                     /locus_tag="PC700_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2581847.1"
                     /translation="MTIKKLPQSELKIMKFIWKSDSKVTSRDIVLGMEQKYQWKQTTT
                     LTLLSRLVVKRFLNSQKIDKYTHYEVLIKEKEYIGVETRDFFRNIHDSSIKSLLLSLH
                     ENINLSKDDILLIEEWIKNLKEEEKDV"
     gene            2049..2519
                     /locus_tag="PC700_002549"
     CDS             2049..2519
                     /locus_tag="PC700_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HDF2581848.1"
                     /translation="MKYQVSLLAVKDIEVSKKFYKELFEQEIELDLGKNVTFKGGFAI
                     QEDFAWLTGINPDTIIQKSNNMELYFEVDDFDEFIEKLNYYKDVEFVHKPLKHEWQQR
                     VVRIYDPDKHIIEIGESMEVIARRYLSEGYSVEETSKIIQHPIEFVKQCENSKE"
     gene            2737..3726
                     /locus_tag="PC700_002550"
     CDS             2737..3726
                     /locus_tag="PC700_002550"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HDF2581849.1"
                     /translation="MFENLPKIDLHCHLDGSVRVETMLDIAIKEKIDLPSNNMDEIKK
                     LAKVSFNCTSLDEYLEKFDLPLKVMQSKENLKRITFELLEDASRENVKYIEIRFAPLL
                     HTQKGMSVKNIIEGIIEGIREAESIYDIKGNLILGCMRTMTSKEALLVIEEGKSFVNK
                     GVVAVDLCGPEKEGFCKEYKDVFKLAREYGYKVTIHAGEAASGENVLDAINILKADRI
                     GHGVKIKDHKKAYNLVKDKKILLELCPTSNVQTKTVDSYEVHPFYTFYKDNLHVSINT
                     DNRTVSDINLSSELNVIFDTFKLGLEDYKIIYRNAVEASFADKETKEYLNSLI"
     gene            3798..4538
                     /locus_tag="PC700_002551"
     CDS             3798..4538
                     /locus_tag="PC700_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HDF2581850.1"
                     /translation="MKDYEDVVYIKLVKKELEEWKKNILKKPSIFDKASKGIQNKFNG
                     VLPDKYHEIITSSIKVMTKTVLFGYKYATKPPLRNMSLEEREKLVLEKAKTYKTTAMI
                     EGAGTGAGGILLGLSDFPLLLGIKVKFLYDVASIYGYDVKDYRERIYILNILQLAFSS
                     QQHINKTFKLMNNWDEYVNSLPEDINDFDWRTFQQEYRDYLDLAKFLQLMPGIGAFVG
                     FYVNGKLIDKLCETAVSAYRLRIRELKL"
     gene            4738..5889
                     /locus_tag="PC700_002552"
     CDS             4738..5889
                     /locus_tag="PC700_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HDF2581851.1"
                     /translation="MTNLPKQFDSFNEARQKGFINAKELKESGKKMVGVFCTFTPVEI
                     PMAAGATVVGVCGVSEEPIPDAERVLPRNLCPLIKSSYGHAITDTCPYFYFSDLLIGE
                     TTCDGKKKMYEELAKVKPTYVMHLPNTSKGEFAYKLWKDEMIRLKEEVEKSLGVTITE
                     EDIRTAIKDKNEERELLKEFYALGKLEPSALTGLELHNVLYQAGFKFDRAELKHSLRK
                     VIDDMKERYEKGECPVQKDKPRILITGSPIGGISEKIVKTLEDAGASVVAYELCGAIR
                     SNDLLVDEEIEDVYDALTQKYINIGCSCMMNNDNRIELLDRIIDEYNVDAVIDVVLQA
                     CHTFNIESYRIREFVTKEKNKPFMSLETDYSKSDTEQLRTRFEAFVEML"
     gene            5914..6669
                     /locus_tag="PC700_002553"
     CDS             5914..6669
                     /locus_tag="PC700_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HDF2581852.1"
                     /translation="MFSIGVDSGSVATKGVLFDGEKIIKKIIIPTGWSPKNTSKQVYE
                     LLSSEIDKKDIKKVVGTGYGRGVMDFADKKVTEITCHTRGIYFLNKNIRTILDVGGQD
                     SKVINLDRDGNVFNFIMNDKCAAGTGRFLEITSNLLGSDIESIDTLAKGYEPVNISSM
                     CTVFAESEIVSLLAQNISTGEVAAGILKSIANKSTSMLARGEVIDEVAFTGGLAKSKE
                     LVKMIEEILGKKIFIAEDTQIIGALGAAVIGFR"
     gene            6861..9248
                     /locus_tag="PC700_002554"
     CDS             6861..9248
                     /locus_tag="PC700_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp13"
                     /protein_id="HDF2581853.1"
                     /translation="MKKFTSKKVARFVALFLISILVMSTIPVSADNSNTLNQIKKAEY
                     SDTYKQYLNDAKNGRTEKYNGIIPNPYQLEGTQIQSKGRTAPTSYDPRKLGLMTSVKN
                     QEDLGICWDFAAMATLESFLKLNNYGDYDLSEEHLRWWASDGEYGWSVNDMDGALNYE
                     AMGYLTSWSGPKLEKDIPYNGRVSKAQGAKKPTNMNTAPTVFNVTDAVCVSNDINSTK
                     NAILQYGAVTSGYYEDIKYQSDDQNSYYCPTKSFNTNHAISIVGWDDNYSKDNFNANI
                     RPSKNGAWLIKNSWGDYNSEGGYFWISYEDKTLMSDIDNFSIKGGKKPNDDEKMYQHD
                     YASIVPLISKKITAANVFDFNRGDETLKSVMFLTESIGAKYEVSYAPVVNGIPQENNM
                     KKLKEGTAQYSGYITVPIDSFNIPEGKGAIVVSIEGKNGESTIGSESNVPGYDTFKAK
                     ANLGESYIIDNTGKFFDINRDSNFYPCNFTIKAVTEKSSGESIPNESLIGSDRYETAI
                     KVSQNGFNSSENVVLVNGSSIVDALAATPFTSAINSPILLTQKEALNSKTKAEIQRLG
                     AKKVYLIGGENSISKEIEQQLKSLNISIERISGSDRYKTSLLLAQKLNGIKNVSQIAV
                     VNGVKGLADAISVGAAAAENNIPIILANEKSELQGADEFLNSLNIEKSYIIGGTASLS
                     NNLESKLKNPTRLSGSSRDETNSKIIDNFYKKDTLKNAFVVKNGIKNQNDLIDGLAVG
                     PLGAKTGSPVILVGDKLADSQKEVLKNKTLEKVTQVGGGANKNAFKELVKLKSAK"
BASE COUNT         3897 a          864 c         1736 g         2890 t
ORIGIN      
        1 ttaaacaagg caaaaactat aatcttaaaa ttaaaattaa cattattctt aagaagtaag
       61 aaatttaaga agtaaaaagt ttagtgataa gtggtagtaa gcaagaaatt tagaaagtag
      121 gaagtttagt gataagtggt agtaagcgag aaatttagaa agtgggaagt ttagtaataa
      181 tttgtagcaa gtaagaaatt taggaagtag gaagtttagt aataatttgt agcaagtaag
      241 aaatttaggg agtaggaagt ttagtagtaa taatttgtag caagtaagaa atttagggag
      301 taggaagttt agtaataatt tgtagtaagt aagaaattta gaaagtagga aatttagtaa
      361 taatttatag taagtaagaa atttagaaag taggaagttt agtaataatt tatagcaagt
      421 aagaaattta gaaagtagga agtttagtag taatttatag taagtaagaa atttagtaag
      481 tgagaagttt agttataatt tatagtaagt aagaaattta gtaagtgaga agttcagtaa
      541 taagttgtag taaatgaaaa gtttaggaag taggaagttc agtaataagt tgtagtaaat
      601 aaaaaattta ggaagtagga tttactgaaa tacaaatatt taaaatcaaa gagtttaaaa
      661 gaaaggagag ctactggaca agtatccaaa atagatagct gagtacaaaa tatctcataa
      721 aattaagcaa tataatacaa gagaattttg acattattta tatatattta ccgtgtacaa
      781 atagctaaat ttaggattaa gattgaaaaa tatcttataa gtctgagctt atataaatcc
      841 gtataagatg tatttaatcg aaaaatttta ttatggatat tgcttgtcta tattctgagt
      901 agcaaggttt tatcatgact ataaaaaagt tgccacaatc agaattaaag ataatgaaat
      961 ttatatggaa atcagattcc aaagtaacat ctagagatat tgttttagga atggagcaaa
     1021 aatatcaatg gaaacaaacg acaactttga ctcttttatc tagattagta gtaaaaagat
     1081 ttctaaattc tcaaaagata gataaatata cacattatga agttttaatt aaagaaaagg
     1141 aatacatagg agttgagaca agagattttt ttagaaatat tcatgattct tctataaaaa
     1201 gtttattgtt gtcattacat gaaaatatta atttgagcaa agatgatatc ttacttattg
     1261 aagagtggat aaaaaatcta aaagaagagg agaaagacgt ataagcaaac gtatttctta
     1321 tagcattaat ataaagtgta tgtaagtgta tttatgtaat atattagtct acttaagtaa
     1381 tatgacaaat gattataagt ggattatgat actataatgt gaatctaaat aaattaaata
     1441 taaattgata gtggatattc tatttaatta aatttaaaaa gctaccttta cttataagta
     1501 taattaaggt agctttttaa atagaggtag tgtgattaaa gagaattttt aaatataaat
     1561 tgtgaagctt taatatagat gacaatatat attttgcaaa tatgacacag tgttttcgta
     1621 tttaagatga taaaataaat aggaattaat gaagatattt aaaaaataaa aataaagaat
     1681 taatagaaga tattaaagag atagagataa aagtgttaat agaagatatt gaaaaagtaa
     1741 aaataaagga ttaatagaag atactgaaga ggtagaaaca aaagtgttaa tagaagatat
     1801 tgaaaaaata aaaacaaaag attaataaaa ggtactgaag agatagagat aaaagtgtta
     1861 atagaagata ttgaaaaata aaaataaaag attgatataa tatactgaag aggtagaaac
     1921 aaaagtgtta atagaagata ctaaaaaagt aaaaataaag gactaataaa aggtactgaa
     1981 gagatagaga aaaaaatgtt aatagaagat actgaaaaaa tagaaataaa aaattaggag
     2041 gaaaatttat gaaatatcag gtttcattat tagcagtgaa ggatattgaa gtatcaaaaa
     2101 aattttacaa agaattattt gagcaagaaa ttgaattgga cttaggcaaa aatgtaacat
     2161 ttaaaggagg atttgctata caagaagact tcgcatggct tacagggatt aatccagata
     2221 caattataca aaaatcaaat aatatggaac tttattttga ggttgatgat tttgacgaat
     2281 ttattgaaaa actcaattat tataaagatg tagagtttgt ccataagcca ctaaaacatg
     2341 aatggcaaca aagagtagtg cgtatttatg acccagataa gcatattatt gagataggag
     2401 agtctatgga agttatagct agaagatatt tatctgaggg atactctgta gaagaaactt
     2461 ctaaaataat tcaacatcca attgaatttg taaagcagtg tgaaaatagt aaggaatgat
     2521 tgtttaaatt ataattattt agtagaaatt aattgtagtg gaactaattc aagataatat
     2581 ataggactag tatttgacaa atgtcataaa ttaaattaaa atattttaat gagatgtgac
     2641 ttttttagaa tgaaaaaccg tagtatattg atataataga aataagactt aaaaatctgt
     2701 tgatagtatt atttataagt acagtaggag gataaaatgt ttgaaaattt acctaagata
     2761 gatttacatt gccacttaga tggaagtgtt agagtagaaa ccatgcttga tatagctatt
     2821 aaagagaaaa tagatttacc ttcaaataat atggatgaaa taaaaaaatt agcaaaggtt
     2881 tcttttaatt gtacttcact agatgaatat ttagaaaaat ttgatttacc cttaaaagta
     2941 atgcaatcta aggaaaattt aaaaagaatt acttttgaac ttttggaaga tgcaagtaga
     3001 gaaaatgtaa aatatattga aattagattt gcaccattac ttcacaccca aaaaggtatg
     3061 agtgttaaaa atataattga gggtataatt gaaggaatta gagaagctga aagtatttat
     3121 gatataaaag gcaatttaat attaggttgt atgagaacaa tgacctcaaa agaggcatta
     3181 ttagtaattg aagaagggaa atcatttgta aataaaggtg ttgtagcagt agacttgtgt
     3241 ggtccagaga aagagggatt ttgtaaggaa tataaagatg tttttaaatt agcaagagaa
     3301 tatggatata aagttacaat acatgcagga gaagctgcaa gtggagaaaa tgtattggat
     3361 gctataaata ttttaaaggc ggataggatt ggtcatgggg taaaaattaa agaccataaa
     3421 aaggcgtata atttagttaa agataaaaaa atattgcttg aattatgtcc aactagtaat
     3481 gtacaaacta agacagtaga ttcttatgag gtacatccat tttatacttt ttataaagat
     3541 aatttacatg taagtataaa tactgataat agaactgtat cagatataaa tttaagttca
     3601 gaactaaatg ttatatttga tacttttaaa ctaggattag aagactataa aattatttat
     3661 agaaatgcag tagaagctag ttttgcagat aaagaaacta aagagtattt gaatagttta
     3721 atttaataga taaaaatatt taaactttaa attaagctat taaattgaca aaaatacatt
     3781 gtattaggag aaaaaatgtg aaagattatg aagatgttgt ttatattaaa ctagtcaaaa
     3841 aagaacttga agaatggaag aaaaatatat taaagaaacc ttctatattt gacaaagcat
     3901 caaagggtat acagaataaa tttaatggag tattgccaga taaatatcat gaaataatta
     3961 cttcttctat aaaagtaatg acaaaaaccg tactgtttgg atataaatat gctacaaagc
     4021 caccacttag aaatatgtca ttagaagaaa gagaaaaatt agttttagaa aaagctaaaa
     4081 cttacaagac cacagcaatg attgaagggg caggaacagg ggcaggagga atcctcttag
     4141 gtttatctga ttttcctttg cttttaggta ttaaagtgaa atttttatat gatgtagctt
     4201 caatatatgg atatgatgtt aaagattata gagaaaggat ttatatatta aacatattac
     4261 agttggcttt ttctagtcaa cagcatataa acaaaacttt taaattgatg aataactggg
     4321 atgaatatgt aaatagtcta ccagaagaca tcaatgattt tgattggaga acatttcaac
     4381 aagagtatag agattattta gatttagcta agtttttaca actaatgcct ggaataggag
     4441 catttgtagg attttatgtg aatggaaaat taattgataa attatgtgaa actgctgtgt
     4501 ctgcttatag attgaggatt cgagagctta agttataaga gaaattataa cataaaaaat
     4561 tagttataca atatatctat atgtatctaa ttttgcattg ataaaaacaa ttatggataa
     4621 aaggtaaagc atgatagaat ctaatgtaga ggtttgtatt ttaatggaaa aatttagttt
     4681 ttattgctta agatacaaat tgaattacga agagaagtaa ttgggaggaa tattttcatg
     4741 acaaatttgc caaaacaatt tgatagcttc aatgaagcta gacaaaaggg atttataaat
     4801 gcaaaggaac ttaaggaaag tgggaaaaag atggttggag ttttctgtac atttactcca
     4861 gttgaaattc caatggcagc tggagcaact gtagtaggtg tatgcggtgt tagtgaagag
     4921 ccaatacctg atgcagaaag ggtgcttcca agaaatcttt gtcctttaat taaatcaagc
     4981 tatggacatg caataacaga cacctgtcct tatttctatt tttctgattt gttgatagga
     5041 gaaaccactt gtgatggtaa gaaaaaaatg tatgaagaac ttgcaaaagt taagccaaca
     5101 tatgtaatgc atttgccaaa tacttcaaaa ggagaatttg cctataaact ttggaaagat
     5161 gaaatgataa gattaaaaga agaggttgag aaatctttag gtgtaactat aactgaagaa
     5221 gatataagaa ctgctataaa agataaaaat gaagaaagag aacttttaaa agagttttat
     5281 gcacttggta aattagaacc atctgcactt acaggccttg agttacataa tgttttatat
     5341 caagcaggtt ttaaatttga ccgtgcagaa cttaaacatt ctttaagaaa agttatagat
     5401 gatatgaaag aaagatatga aaaaggtgaa tgtcctgttc aaaaagataa gccaagaata
     5461 ttgataacag gttctcctat tggtggaata agcgaaaaaa tagttaaaac tttagaagat
     5521 gcaggggcat cagtcgttgc atatgaactt tgtggagcta ttagatcaaa cgatttattg
     5581 gttgatgaag aaatagaaga tgtatatgat gctttaacac aaaaatatat aaatataggt
     5641 tgttcttgta tgatgaataa tgacaatcgt attgaattat tagatagaat aatagatgaa
     5701 tataatgtag atgctgtaat agatgtagtt ttacaagcat gtcatacatt taatatagaa
     5761 agttatagaa taagagaatt tgttacaaaa gaaaaaaata agccatttat gtctcttgaa
     5821 actgattatt caaaatcaga tactgaacaa ttaagaacta gatttgaagc gtttgtagag
     5881 atgttgtagg aataaataaa agtaggagga actatgttta gtataggagt tgactcaggg
     5941 tcagtggcaa caaagggtgt gctatttgat ggtgaaaaaa ttattaagaa aataattata
     6001 ccaactggat ggagccctaa aaatacatct aaacaggtct atgagttgct aagtagtgaa
     6061 attgacaaaa aagatataaa aaaggtggta ggtacaggtt atggaagggg agtaatggat
     6121 tttgctgata agaaagtaac agaaataact tgtcatacta gaggtattta ctttttaaac
     6181 aagaacataa ggactatttt agatgtaggt ggtcaagata gtaaagttat aaatttagat
     6241 agagatggaa atgtatttaa ctttataatg aatgataagt gtgctgcagg aactggtcga
     6301 tttttagaga ttacttctaa ccttttagga agcgatattg aaagtataga tacattagct
     6361 aaaggatatg aaccagttaa tatatctagt atgtgtacag tttttgctga gtctgaaatt
     6421 gtaagtcttc tagctcaaaa catttctact ggagaggtag cagcagggat acttaaatct
     6481 attgcaaata aatccacctc aatgttagca agaggtgagg taatagatga agttgcattt
     6541 actggagggc ttgcaaaaag taaagaacta gtaaaaatga tagaagaaat actgggtaaa
     6601 aaaattttta ttgcagaaga tactcaaata ataggagctt taggagctgc tgtaatagga
     6661 tttagataaa aacatataaa aagaaaatga aaagctgaat ttatcataaa taagttcagt
     6721 tttttatttt attttgttta tttataaagg aaaactttgg tataattgat ttaaatgatt
     6781 gtatatttgt aaatacttga tatttagtca tatataacat tagaatttta aaaattagct
     6841 caaaataagg gggagaaagc gtgaaaaaat ttacttcaaa aaaagtagca aggtttgttg
     6901 cactgttttt aatatctatt ttagtaatgt caacaattcc tgtgtcagct gacaatagca
     6961 atacattgaa tcagataaaa aaagcagaat acagtgatac ttataaacaa tatctaaatg
     7021 atgctaaaaa tggacgtaca gaaaaatata atggaattat accaaatcca tatcagttag
     7081 aaggtactca aattcaaagt aaaggaagaa cagctccaac atcttatgac cctagaaaat
     7141 taggtcttat gacatcagta aaaaatcaag aggatttagg aatatgctgg gattttgctg
     7201 caatggcaac cttggaaagt ttcttgaagt taaataatta tggagactac gatttatcag
     7261 aagaacactt aagatggtgg gcttcagatg gtgaatatgg atggtctgta aatgatatgg
     7321 atggtgcttt aaattatgaa gctatgggat atttaacatc ttggtcaggt cctaaattag
     7381 aaaaagatat cccatacaat ggtagagtat ctaaagcaca aggagcaaaa aaaccaacta
     7441 atatgaatac agcacctact gtttttaatg tgacagatgc tgtgtgtgta agtaatgaca
     7501 taaattctac aaaaaatgct atattacagt atggagcagt aacatctggt tattatgaag
     7561 atattaaata tcaaagtgat gaccaaaatt catattattg tccaacaaaa agttttaata
     7621 caaatcatgc tatttcaatt gtgggttggg atgataacta ttctaaagat aattttaatg
     7681 caaatattag accatcaaaa aatggtgctt ggttaataaa aaatagctgg ggagattata
     7741 attctgaggg tggatatttc tggatatctt atgaagacaa gacacttatg tctgatatag
     7801 ataatttttc aatcaaaggt ggtaaaaaac caaatgatga tgagaagatg tatcaacatg
     7861 actatgcaag tattgtacct ttaatttcaa aaaagataac agctgcaaat gtatttgatt
     7921 tcaatagagg cgatgaaact ctaaaatctg taatgttctt aactgaaagt ataggagcta
     7981 agtatgaagt atcttatgct ccagtagtaa atggtatacc tcaagaaaac aatatgaaaa
     8041 aattaaaaga aggtactgcc caatattcag gatacataac agttccaatt gattctttta
     8101 atataccaga aggaaagggt gcaattgtag tcagtataga ggggaaaaat ggagagtcaa
     8161 ctataggttc tgagtcaaat gtaccaggat atgatacatt taaagcaaaa gctaatttag
     8221 gtgaaagtta tatcatagat aatactggaa aattctttga tataaataga gatagtaact
     8281 tctatccgtg taattttact ataaaagctg ttacagaaaa gtcttctggt gagagtatcc
     8341 cgaatgagtc tttaattggt agtgatagat atgaaactgc tatcaaagtt agtcaaaatg
     8401 gtttcaattc ttcagaaaat gtagttttag taaatggcag ttcaatagta gatgcattgg
     8461 cagcaactcc ttttacatct gctataaatt caccaatatt attgacacaa aaagaagctt
     8521 taaattcaaa aactaaagct gagatacaga gattaggtgc taagaaagta tacctaatag
     8581 gcggagaaaa ttctataagt aaagaaattg aacaacaatt aaaaagctta aatatatcga
     8641 tagagagaat atcaggaagt gatagataca agacaagttt attattagct caaaagttaa
     8701 atggtataaa aaatgtatct caaatagcag ttgtaaatgg agtgaaagga ttagctgatg
     8761 caattagtgt aggtgctgct gcagcagaaa ataacatacc aatcatactt gcaaatgaaa
     8821 agagcgaatt gcaaggagct gatgaatttc taaattcatt aaatatagag aaatcttata
     8881 ttattggagg aacagcatcc ctatcaaaca atctagaaag taagttaaaa aatccaacta
     8941 gattatctgg tagtagtaga gacgaaacta attcaaagat aatagataat ttttataaaa
     9001 aagatacttt aaaaaatgca tttgtagtta aaaatggaat caagaatcaa aatgatttaa
     9061 tagatggatt ggcagttggt cctttaggag caaaaactgg gtcaccagta attttagttg
     9121 gtgataaatt agctgatagc caaaaagaag ttcttaaaaa taagacttta gaaaaagtaa
     9181 ctcaagttgg tggaggagca aataaaaatg catttaaaga gttagtaaaa ttaaaaagtg
     9241 ctaaatagag tacttaagta ttaacatata ttagtgtgta ttagtaactt atcttaataa
     9301 aagctgaaaa taattacttt aaattattga taaataagat aatcaatgat tgtaaagttt
     9361 tgttttcagc tttaaattta tgtagta
//
LOCUS       DAMJWP010000136         9384 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.141, whole genome shotgun sequence.
ACCESSION   DAMJWP010000136 DAMJWP010000000
VERSION     DAMJWP010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9384
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.141"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(384..1787)
                     /locus_tag="PC700_002555"
     CDS             complement(384..1787)
                     /locus_tag="PC700_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2581854.1"
                     /translation="MKMLNNFFKKINNPAPVFAQTKMERDNQTDDSLSTKPKTTFRDV
                     AGLDEVKEELFEIVDFMKSPQKYQKMGAKIPKGVLFYGPPGTGKTLLASAVAGETNSS
                     FFNVTGSEFVEKYVGVGAKRVRTLFEKARKEAPSIIFIDEIDAVGAKRHLESNNEKDQ
                     TLNQLLVEMDGFNKDSNVLIIGATNRLDLLDEALLRPGRFDRHIHIGAPNYHTRFEIL
                     KVHTDDKPIDKSVNLELLAKKTHGFNGAHLSNIANEAAIFAVRDDSECITSEHFDKAL
                     ERVIAGLESKNSALVEKEKKIVAYHEAGHALVSDIVGICPIQKISIVPRGQALGYVLQ
                     LPDEDRYIYTKDELIGKIKILLAGKASEELIFNHKSTGAKDDLKKVTEIANQMVCEYG
                     MSNLGFMTIDGNDKTFLCDKVQKEANRIVEICYKETLEMLKDNLEDLHSVSKFLFEKE
                     TMTHEELKDLIGKEAVN"
     gene            2664..3173
                     /locus_tag="PC700_002556"
     CDS             2664..3173
                     /locus_tag="PC700_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009528606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDF2581855.1"
                     /translation="MSKELAINEQIKDKEIRVLSPTGEQLGVMPTKEAQAMANSKNLD
                     LVQISPNANPPVCKIMDYGKFRYEQARKEKEAKKKQKTIVVKEVRLRPGIEQNDLNTK
                     ANNAIKFLKKGDKVKVELRFRGRELGHKDIGKEVMLKFLDIIKEFGEPTKAPAFEGNN
                     MVVIIDPKK"
     gene            3201..3395
                     /gene="rpmI"
                     /locus_tag="PC700_002557"
     CDS             3201..3395
                     /gene="rpmI"
                     /locus_tag="PC700_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDF2581856.1"
                     /translation="MPKMKTHRGAAKRLKKTGTGKLKRAKAFKKHILTKKSAKTKMNL
                     RKSTLVSDGDAKRIAQLLPY"
     gene            3451..3807
                     /gene="rplT"
                     /locus_tag="PC700_002558"
     CDS             3451..3807
                     /gene="rplT"
                     /locus_tag="PC700_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDF2581857.1"
                     /translation="MARVKKAMNARKKHKKILKLAKGFRGSRSKLYRPANTFVMKALK
                     NAYIGRKLKKRDFRKLWIQRINAAARMNGISYSRLMNGLKLSGIEVNRKMLSEMAIQD
                     PEGFAKLAEVAKAKLA"
     gene            4117..4662
                     /locus_tag="PC700_002559"
     CDS             4117..4662
                     /locus_tag="PC700_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581858.1"
                     /translation="MNEKLMDCFVNPIKCKLLLEVFSNNRTTAKQLGEKFQDISKATL
                     YRYLNKMLKDDIIKVVEENKIRGTVEKVYAMKLDIADDVNKIIDENSGEEYAQLFTQY
                     MMGFLREFYEYAERKEKDILKEGSGFSVCPVYASIDEIVEVSKKIASILEPLRNNSHE
                     EGRRMHSIGVIITPPKEDEGK"
     gene            4649..4822
                     /locus_tag="PC700_002560"
     CDS             4649..4822
                     /locus_tag="PC700_002560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581859.1"
                     /translation="MKGNKSDLHEVEPIYTENRFMIYICIQSKVSLHGLYFIVPFHPM
                     TWSGTMKFFNFSY"
     gene            5098..7023
                     /locus_tag="PC700_002561"
     CDS             5098..7023
                     /locus_tag="PC700_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2581860.1"
                     /translation="MKIKFVIMPLVIFLIVLFVGCQSTKDTEVKAKDTEIKILGTGDL
                     HSILTDSMVSYVNEEREKNKNLLMVDAGDFYGTQSREMWEWSSGKKLINIKRNGRAEY
                     DDIIKSSKDEVPIVKDMAKLKYDAVVLGDNEFISNDKQSLDKLVSDFKNNNMPLVSAN
                     IYEQSGENYVQPYVMKNIKTDEGNVKVGILGLTIKEVGETLGLEDFEKDKKARELGEQ
                     ADYKGKLYANDLVEDAKKWIKVMEKESPDIIVAVVHSGEEPKTDRNPGNRIKELATTV
                     DGIDAIVAGHTHEKIDEHKYKNKSGDEVIVTQPGWHGDRVSEINFKLNKENGKWDIKE
                     KSSKLKIIDKEVSIVATADLHSHFSDKLTVDLVNERSNPIEPVVVDAGDFLDPQTDEM
                     TQWYKEWKSIRDNNSDKTISRCPMVMDMNQGMYDAVVLGNHEFVSEKDEFWSDERLLD
                     AVIEDFEQVAISVLSANIYRQSGKNYVKPYIIKDVETSEGNVKVGILGLTIKEVGKGL
                     KNVNLQEQFQYKDKLYANDLVEDAKKWVKVMKKKKPDIIVAVVHSGEEPKNPKHPGNR
                     IKELATTVDGIDAIVASHTHEKIDEHEYKNKSGEKVIVTQPGEHGKYYSKITFKVNKE
                     KGSWIINDKFSKTIEVE"
     gene            7424..9328
                     /locus_tag="PC700_002562"
     CDS             7424..9328
                     /locus_tag="PC700_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2581861.1"
                     /translation="MKIKFIIVASIIFLIVLLVGCQSTKDTEINVLATSDLHSIIPDN
                     LISYVEEERKYDENLLMVDAGDFFDMQSGEMNKWFTGQKLVRFKDGIPEYKKIAEPRE
                     GEVPLAKKMAKLKYDAVTFGNHEFVSNDKQSLDKLVSDFKNNNIPLVSANVYEQSGEN
                     YVKPYVMKNINTDKGTVKVGILGLTIKEVGERSRFKSFKQDKKARELGEQPQYKGKLY
                     ANDLVEDANKWVKVMEKKEKPDIIVAIVHSGEEPKNPKNPGNRIKELATTVEGIDAIV
                     AGHTHEKIEQHTYKNNSGEEVIVTQPGAHGNSISKINFKLDKKNDKWFIKDKYSELKV
                     FGKEVNIITTSGLNPKFSDEMAVNLFNERDRDVQPIFIDTGDFLNTNTKEMTKWAKEW
                     QYNENKGIYKKINEWPIVYLGYDAVVLGSHEFTEDKETFEGKKSTLDYIIENFEEMKI
                     PVLSANIYDESGENYVKPYIIHNVETREGNIKVGVLGLTTTDKDEEDLSSSSLSKDSK
                     TSKIPEETGKLYTNNLVKDASEWVKVMQKESPDVIVAVVHSDNNSTLKNPSSEVKKLA
                     MSVDGIDAIVVGHTKNKVEEHIYKNKSGDEVIVTQSGEDCYSKISLELRKENGKWNII
                     NKYSKAIKMK"
BASE COUNT         3751 a         1041 c         1641 g         2951 t
ORIGIN      
        1 aagtgataga tacctaatta agttgtagat atctgataag aaatttttaa gttttcaatc
       61 gttctaaatt gtgagtataa aaatctacta aatttagtta tgtaaaatat aaattataag
      121 tgtagaaact aggctactag cttttatacc acttcaaaca ttactggaag tagttaagtt
      181 tacagcagtt tagttttatg tagatataat attctgtaaa tagacaaaat taaaatatgt
      241 aattaaaata tgtaagtgtc tcaaaatgaa atttttaatt tatgaggcac ttttttgtat
      301 aaaatcaaat atatttttat aactttaaca ataaaaagag gcttatctct attttgagac
      361 agcctcaaat ttttaaaact atattagttt acagcttctt ttcctatcaa atcttttaac
      421 tcttcatgag tcatagtttc tttttcaaat aagaatttag aaacagaatg taaatcttca
      481 agattatcct ttaacatctc taaagtctct ttataacaaa tttcaactat acgatttgct
      541 tccttttgaa ccttatcaca taagaaagtc ttatcatttc catcaatagt cataaatcct
      601 aagttactca taccatattc acaaaccatt tggttagcta tttcagtgac tttctttaag
      661 tcgtcctttg caccagtaga tttatgatta aatattaatt cttctgaagc ctttccagca
      721 agtaatattt ttatttttcc aatcaactca tccttagtat aaatatatct atcttcatca
      781 ggtaattgaa gaacataacc taatgcttgt cctctaggaa ctatagatat tttttgtata
      841 ggacaaattc ctactatatc acttacaaga gcatgtccag cttcatgata tgctactatt
      901 tttttctctt tttcaaccaa tgcagagttt ttagattcta gccctgcaat aactctttca
      961 agtgccttgt caaaatgttc tgatgtaata cactcactgt catctctaac tgcaaatata
     1021 gctgcttcat ttgctatatt tgaaagatga gccccattga aaccatgagt ttttttagct
     1081 aaaagttcta gatttacaga tttgtctata ggtttgtcat ctgtatgtac ctttagaatt
     1141 tcaaatctag tgtgatagtt tggtgcacct atatgaatgt gtctgtcaaa tcttcctggt
     1201 ctaagtaaag cttcatctaa taagtcaagt ctattggtag cacctattat taatacattg
     1261 gaatctttat taaatccatc catttcaact aaaagttggt ttagtgtttg gtctttttcg
     1321 ttgttacttt caagatgtct ctttgctcct acagcatcta tttcgtctat aaatattata
     1381 cttggagctt cttttctagc tttttcaaat agagttctaa ctctttttgc tcccacacct
     1441 acatattttt caacgaactc tgaaccagta acattgaaaa atgatgagtt tgtttcacca
     1501 gctacagcag atgcaagtag ggttttacct gttcctggag gaccataaaa taatactcct
     1561 ttaggtattt ttgcacccat tttttgatat ttttgtggtg acttcataaa atcgactatt
     1621 tcaaatagtt cttcttttac ttcgtctaat ccagcaacgt ctctaaatgt agtctttggt
     1681 tttgtagaaa gtgaatcatc tgtttgatta tctctttcca tttttgtctg tgcaaagaca
     1741 ggagctggat tgttgatttt cttaaaaaag ttgtttagca ttttcatatt cacacctcag
     1801 gtaattaatt attgttaatc aattttatat tttccatatt tactattttt tattatttat
     1861 ttttcatgga aaaaatatac atgttatcta aataaaaata aatttgtaag atgtatgaaa
     1921 aaatgaattt atgtatatag agatttctac ttatgtattg aataaagtat tacaaattat
     1981 aaattatgta ttgattatat attgagtaaa gtattataaa gcatgtattg attaagtatt
     2041 tattaagtat tgaataaaat attacaaatt tagttgaaat gaaaatgata aaaaatgttg
     2101 tgctatattt tacaaatcat ataaatagag aaattgttgt agcacaaagt aataggtagt
     2161 atattagaat ataatatagg atttgttgtg tagtatagta gcatattaga atataatata
     2221 ggatttgtta tgtagtatag tagcatatta gaatataata taggatttgt tatgtatata
     2281 gtagcatatt agaatataat gtaggatttg ttatgtagaa tatatagagt catgtagaat
     2341 ataaatatat aaaaggattt tgggtataaa tttaaaaatt aatagcaaaa tgagtagtat
     2401 agacatataa ttagtagtag gaagtaatta aatttttcct aaacaaaaaa aatagttgca
     2461 aataatttga ttaagtgata tagtaaataa ggtaagaata aaacaaagaa gaagtccgct
     2521 tctcacctta cgccggaggt tagtaaggta aatataagtt aaagcttaat tttaatttaa
     2581 gtttgtttaa atttggtaac ttatattgcg gatgattacg tcatccgctt tttattttat
     2641 aaaaattttg gaggtgtcct acaattagca aagaattagc aatcaatgaa caaataaaag
     2701 ataaggaaat aagagtcctt tcaccaacag gtgaacaact tggagtaatg cctactaaag
     2761 aagctcaagc aatggctaat agcaaaaact tagatttggt tcaaatctcg ccgaatgcga
     2821 atccaccagt atgtaagata atggattatg gaaagtttag atatgagcaa gcgagaaaag
     2881 agaaagaagc taagaaaaaa caaaaaacta tagttgtaaa agaagtaaga cttagaccag
     2941 gtattgaaca aaatgattta aatacgaaag ctaataatgc tataaagttc cttaaaaaag
     3001 gggataaagt caaagttgaa cttagattta gaggaagaga attaggacat aaagacatag
     3061 gtaaagaagt tatgcttaag tttttagata taataaaaga gtttggagaa ccaacaaaag
     3121 cccctgcatt tgagggtaat aatatggttg taattataga tccaaaaaaa tagatgatta
     3181 actgagagga ggaatttgtc atgcctaaaa tgaaaactca tagaggtgca gcaaagagat
     3241 taaagaaaac aggaactgga aagttaaaga gagctaaagc ttttaaaaaa catatattaa
     3301 ctaaaaaatc tgcaaaaact aaaatgaact taagaaaatc aactttagta agtgatggag
     3361 atgccaagag aatagcacaa ttattaccat actaagatac agattgtaaa taataactat
     3421 agaagaggga tataaaagga ggtcataacg atggcaagag ttaaaaaagc tatgaacgct
     3481 cgtaaaaagc ataagaaaat attaaaactt gcaaaaggat tcagaggatc tagaagcaaa
     3541 ttatatagac cagcaaatac attcgtaatg aaagcattaa aaaatgctta tataggaaga
     3601 aagttaaaga agagagactt cagaaaactt tggatacaaa gaataaatgc agctgctaga
     3661 atgaatggaa tatcttattc aagattaatg aatggattaa aattatctgg tattgaagtt
     3721 aacagaaaaa tgttatctga aatggctata caagacccag aaggatttgc aaaattagca
     3781 gaagttgcta aagcaaaatt agcttagtat tgaatcatga gatgtaaagt ctttgagtta
     3841 tataaataat tcaaggactt ttttattttt tgaaaaatac ttattagtgt catgtacaac
     3901 caattttata atgataaata aaataagcaa tacaatagaa ttttatgaga tttattataa
     3961 gaattgttac aattatttat catagctata aaaagtcata gtaaaaatga tattgatgta
     4021 tgtttatata tgtataaagc tttcaatcaa tcttgaatta atattatcaa catgataata
     4081 ttatcttatt aataatataa ataaggggga gataatatga atgagaaact aatggattgt
     4141 tttgtaaacc caattaaatg caagttattg cttgaagttt tttcaaataa tcgaactaca
     4201 gctaaacaat taggagaaaa atttcaagat atatcaaagg caacacttta tcgttacctt
     4261 aataaaatgt tgaaagatga tattataaaa gtggttgaag aaaataaaat aaggggaact
     4321 gttgaaaaag tatatgctat gaagttagat atagctgatg atgtgaacaa aattatagat
     4381 gagaactctg gagaagaata tgctcaactg tttactcaat atatgatggg atttttaaga
     4441 gaattttatg aatacgctga aagaaaggaa aaggatatcc taaaagaggg ttcaggattt
     4501 tctgtctgtc cagtatatgc tagtattgat gaaatagttg aagtgagtaa aaaaattgct
     4561 agtatactag aacctctaag aaataactcg cacgaagaag gcagaagaat gcattctata
     4621 ggagtgataa tcactccacc taaagaggat gaagggaaat aaatctgatt tacatgaggt
     4681 agagccaatt tacacggaaa atcgttttat gatttacata tgtatccaga gcaaagtatc
     4741 tctgcatgga ttgtacttca ttgtaccatt tcatccaatg acttggagtg gaacaatgaa
     4801 gttttttaat ttttcatatt aatgcctaaa gaaatgaact ttagaccaca tatgcaatta
     4861 ggctacatta tacaattaga ccagatatac agaataaaaa ttagtatttt tatagcagat
     4921 aaattgactg ttattcttct taaaaatcat ctattagtta ttgttagcat attgaaagaa
     4981 attatagtaa tccattgaat aaatgaatat tgcccataat catataaact gctatagttt
     5041 aaaataaagc aggacgttaa attttatagt aatcttaaaa tttaagaggg ggattttatg
     5101 aaaataaaat ttgttattat gccattggtg atatttttaa tagtattatt tgtggggtgt
     5161 caaagtacaa aagatacaga agttaaagca aaagatacag aaattaaaat attaggtaca
     5221 ggtgatttgc attcaatttt gacagactct atggtttcat atgtaaatga ggaaagggaa
     5281 aagaataaaa atctgcttat ggtagatgcg ggagatttct atggtacaca aagtagagaa
     5341 atgtgggaat ggtcttctgg aaaaaagtta attaatatta aaagaaatgg tagagcagag
     5401 tatgatgaca taattaaaag tagtaaagat gaagtaccta tagttaaaga tatggcaaag
     5461 ttgaaatatg atgctgtagt tttgggagat aatgaattta tttcaaatga taagcaaagt
     5521 cttgataaac tagtttctga ttttaaaaat aataatatgc cattggtatc agcaaatata
     5581 tatgaacaat ctggagaaaa ttatgtacaa ccatatgtaa tgaaaaatat taagacagat
     5641 gagggcaatg taaaagttgg aatactaggt cttactataa aagaagtagg ggaaaccctt
     5701 ggtcttgagg attttgaaaa ggataaaaaa gctagagagc ttggagaaca agctgattac
     5761 aaaggaaaat tatatgcaaa tgatttagta gaagatgcaa agaaatggat aaaagttatg
     5821 gaaaaagaaa gtccagacat aatagttgca gttgtacact caggggaaga acctaaaaca
     5881 gatagaaatc caggaaatag aataaaagag ttagcaacta cagtggatgg aatagatgca
     5941 attgtagctg gtcatactca tgaaaaaata gatgaacaca aatataaaaa taaatctgga
     6001 gatgaagtaa ttgttacaca accaggttgg catggagata gagtatcaga gattaatttc
     6061 aaattaaata aagagaatgg aaagtgggac ataaaggaaa agtctagtaa attaaaaata
     6121 attgataaag aagtaagtat agttgctact gcagatttac attcacattt ctctgataaa
     6181 ttaacagttg accttgttaa tgaacgtagt aacccaattg aacctgttgt agtagatgca
     6241 ggcgacttct tagaccctca aacagatgaa atgactcaat ggtataaaga gtggaaaagt
     6301 ataagagata ataatagtga taaaacgatt agtagatgcc caatggtcat ggatatgaat
     6361 cagggaatgt atgatgcagt agtacttgga aatcatgaat ttgtatctga aaaagatgaa
     6421 ttttggtcag atgaacgcct tttagatgct gttatagagg attttgaaca ggtggcaatt
     6481 tctgtgttat cagcaaatat atataggcaa tctggtaaaa attatgttaa accatatatt
     6541 ataaaagatg tggagacgag tgaaggtaat gtaaaagtag gaatattggg acttactata
     6601 aaagaagtag gtaaaggtct taaaaatgta aacttacaag agcaatttca atataaggat
     6661 aaattgtatg caaatgattt agtggaagat gcaaagaaat gggtaaaagt tatgaaaaag
     6721 aaaaaaccag atataatagt tgcagttgta cattcaggag aagaaccaaa aaatccaaaa
     6781 cacccaggaa atagaataaa agagctagca actacagtag atggtattga tgcaattgtt
     6841 gctagtcata ctcatgaaaa aatagatgag catgaatata aaaataagtc tggagaaaaa
     6901 gttatagtga cacaaccagg tgagcatggt aaatattatt ctaaaattac ctttaaagta
     6961 aataaagaaa agggttcgtg gattattaat gataaattca gtaaaacaat tgaggttgaa
     7021 taactttata aaatttatag ataaagtaaa ttatctgata ttgacatgat atataagata
     7081 aagtttctaa tattagtata atgcataatg tgaaatattt taataataag agaataaaca
     7141 agacaaataa aaatatttta acatgtaaga tatagtatga actattatac taatgcttac
     7201 tttataattt gaacatgtta gaataagtct tatttgaaaa ttttagtgta ttagagtaag
     7261 tattatttta ccagtaaaaa atattgagaa ctatatgtag atttgcttaa aaaggaattt
     7321 acatatagtt cttttcgtat aataaatagt acaatcttat atggtaaaga taaagtgtga
     7381 tattaaattt tattatcatc caaaatctaa ggaggagtct cttatgaaaa taaaatttat
     7441 tatagtagca tcaataatat ttttaatagt attacttgtg gggtgtcaaa gtactaaaga
     7501 cacagaaatc aatgtactcg ccacaagtga tttacattca attattccag ataatttaat
     7561 ttcctatgta gaagaggaga gaaaatatga tgagaatcta cttatggtag atgcaggaga
     7621 tttttttgac atgcaaagtg gtgagatgaa taagtggttt actggtcaaa aattagtaag
     7681 atttaaagat gggataccag agtacaaaaa aatagctgaa ccacgtgaag gagaagttcc
     7741 attggctaaa aaaatggcaa agctgaagta tgatgcagtt acatttggga atcatgaatt
     7801 tgtatctaat gacaagcaga gccttgataa attggtttct gattttaaaa ataacaatat
     7861 accattagta tcagcaaacg tatatgaaca atctggagaa aactacgtaa agccatatgt
     7921 aatgaaaaat ataaatactg acaagggaac tgtaaaggtt ggtatacttg gtctgactat
     7981 aaaagaagta ggggaacgtt caaggtttaa aagttttaaa caagataaaa aggctagaga
     8041 acttggggaa caacctcaat acaaagggaa attatatgca aatgatttag tagaagatgc
     8101 aaataagtgg gtaaaggtta tggaaaaaaa agaaaagcca gatataatag ttgccattgt
     8161 acattcaggt gaagagccaa aaaatcctaa aaatccagga aatagaataa aagaactagc
     8221 aacaactgta gagggaatag atgctattgt agcaggtcat acacatgaaa aaatagaaca
     8281 gcatacatat aaaaataatt ctggtgaaga agtaatagta acacagcctg gagctcatgg
     8341 taatagtata tcaaagatta attttaagtt ggataagaag aatgataagt ggtttataaa
     8401 agacaaatat agtgaattaa aggtttttgg aaaggaagta aatataatta caacttctgg
     8461 tctgaatcca aaattttcag atgaaatggc agttaatttg tttaatgaaa gagatagaga
     8521 tgttcaacct atctttatag atactggaga ttttttaaat actaatacaa aagaaatgac
     8581 taagtgggct aaagagtggc aatacaatga aaataaaggc atctataaaa aaataaatga
     8641 atggccaatt gtatatcttg gttatgatgc tgtagtactt ggtagccatg aatttacaga
     8701 ggataaagag acttttgagg gaaaaaaatc tacattggat tatattatag aaaactttga
     8761 agaaatgaag ataccagtat tatcagctaa tatatatgat gaatctggag aaaattatgt
     8821 aaaaccttat ataattcata atgtagaaac tagggaagga aatataaagg ttggagtatt
     8881 aggtcttact actactgata aggatgaaga agacttaagt agctcaagct taagcaaaga
     8941 ctcaaagact tcgaaaattc ctgaagaaac aggaaagtta tatactaata atctagtaaa
     9001 agatgcaagc gaatgggtaa aagttatgca aaaggaaagt cctgatgtga ttgttgcagt
     9061 tgtacattca gataataata gtactctaaa aaatccaagt agtgaagtca aaaaacttgc
     9121 aatgtcagta gatggaatag atgctattgt tgtaggtcat acgaaaaata aggtagaaga
     9181 acatatttac aaaaataagt ctggagatga ggttatagta acacaatctg gagaagattg
     9241 ttattcaaaa ataagtcttg aactaagaaa agaaaatggc aagtggaata tcataaacaa
     9301 atatagtaaa gcaataaaaa tgaagtgaat ttattgatat ataaaccatt attaacactg
     9361 atttttataa tataataaat actg
//
LOCUS       DAMJWP010000137         9379 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.142, whole genome shotgun sequence.
ACCESSION   DAMJWP010000137 DAMJWP010000000
VERSION     DAMJWP010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9379
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.142"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            50..1810
                     /gene="prdR"
                     /locus_tag="PC700_002563"
     CDS             50..1810
                     /gene="prdR"
                     /locus_tag="PC700_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator
                     PrdR"
                     /protein_id="HDF2581862.1"
                     /translation="MFSIPEVKKVEEVMDTKFTTIDEDTRIEDAIKEMIKSNTKTLMV
                     IDSSDQLKGIISMTDIHNLYEMHKKYEGQPVKLIMKKDVIYVNEGLTLDECRDIMILK
                     NIGILPVLRDNKIIGVLKQEHIRDYLYMHLEDYGLTLKYIIGQIKEGICAINNEGVVI
                     LWNKFMEERYDIKSEDIVGRPMNEFLENTISEKVLNSKVGMSDLYFTDKKENMYALVH
                     ANPIFYKEEFIGVVCTEVDVTEAKILALELEKVNDTLKYLKNEVKNLSKGSFDKILGK
                     SYKLEKSKAIAKQVARTNSSIFIWGESGTGKEVFARAIHDYSERKGQFIPVNCSAIPN
                     ELFESEFFGYESGAFTGASKKGRIGIFELAKDGTVFLDEIADLPLSMQAKLLRVLQEK
                     EIRRVGGDTTIKINPRIISATNKDLSKMVKAEKFREDLYYRLNVVEIKIPPLRERKED
                     IGLLVHSFLEEICKQNNKPVLTISKDVIDIFQNYRWKGNIRELKNTIENIVVLSQNSK
                     IDVDDVPSYMMDSTNNSTEEEEYPLDLTKATQKIEIKNITKALKMSNGNKAKAAKILN
                     IPRTTLYYKIDQYKIDVSKI"
     gene            2239..4119
                     /gene="prdA"
                     /locus_tag="PC700_002564"
     CDS             2239..4119
                     /gene="prdA"
                     /locus_tag="PC700_002564"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-proline reductase (dithiol) proprotein PrdA"
                     /protein_id="HDF2581863.1"
                     /translation="MSITLETAQAHANDPAVCCCRFEAGTIIAPENLEDPAIFADLED
                     SGLLTIPENGLTIGQVLGAKLKETLDALSPMTTDNVEGYKAGEAKEEVVEETVEEAAP
                     VSEAAVVPVSTGVAGETVKIHIGEGKNISLEIPLSVAGQAGVAAPVANVAAPVASAAA
                     EVAPKVEEKKLLRSLTKKHFKIDKVEFADETKIEGTTLYIRNAEEICKEANETQELVV
                     DMKLEIITPDKYETYSEAVLDIQPIATKEEGELGSGITRVIDGAVMVLTGTDEDGVQI
                     GEFGSSEGELNTTIMWGRPGAADKGEIFIKGQVTIKAGTNMERPGPLAAHRAFDYVTQ
                     EIREALKKVDNSLVVDEEVIEQYRREGKKKVVVIKEIMGQGAMHDNLILPVEPVGTLG
                     AQPNVDLGNMPVVLSPLEVLDGGIHALTCIGPASKEMSRHYWREPLVIRAMEDEEIDL
                     VGVVFVGSPQVNAEKFYVSKRLGMLVEAMEVDGAVVTTEGFGNNHIDFASHIEQIGMR
                     GIPVVGVSFSAVQGALVVGNKYMTHMVDNNKSKQGIENEILSNNTLAPEDAVRIMAML
                     KNAIEGVEVKAPERKWNPNVKLNNIEAIEKVTGEKIVLEENEQSLPMSKKRREIYEKD
                     EN"
     gene            4200..4484
                     /locus_tag="PC700_002565"
     CDS             4200..4484
                     /locus_tag="PC700_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581864.1"
                     /translation="MDRLKYISSETFYEGIIVDIKGGGVTIDLKGRLGQFKIPNRMLI
                     TDYELKIGQEVGFMLSYPEVLSPEPNQEYVENIRREKEKQAEMQSKQEEK"
     gene            4522..5247
                     /gene="prdB"
                     /locus_tag="PC700_002566"
     CDS             4522..5247
                     /gene="prdB"
                     /locus_tag="PC700_002566"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:4972..4974,aa:Sec)
                     /transl_table=11
                     /product="D-proline reductase (dithiol) protein PrdB"
                     /protein_id="HDF2581865.1"
                     /translation="MSLTTVQGLQSEIFVPITPPPVWTPVTKELKDMTIALATAAGVH
                     LKSDKRFNLAGDTTFRAIPNTATVDEMMVSHGGYDNGDVNKDINCMFPIDRLHELAAE
                     GFIKGVAPMHYAFMGGGGNQHVFTEETGPAIAAKLKEEGVDGVVMTAGUGTCHRTAVI
                     VQRAIEEAGIPTIIIAALPPVVRQNGTPRAVAPLVPMGANAGEPHNIEMQTHILRDTL
                     EQLVAIPSAGKIVPLPYEYKAHV"
     gene            5327..6085
                     /gene="prdD"
                     /locus_tag="PC700_002567"
     CDS             5327..6085
                     /gene="prdD"
                     /locus_tag="PC700_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase cluster protein PrdD"
                     /protein_id="HDF2581866.1"
                     /translation="MEEKIMRRLVIKPFHMNEVNFGSKTSIKKDVLTIDLSSIDEIKE
                     REELITDIKVDIIKPGDYDREINTIMDIIPISTKVLGRLGEGITHTLTGVYVMLTGAD
                     EDGNQMHEFGSSEGILKDQMVFGRYGTPSVEDYIIHVDVTLKGGLPFERTLPLAAFRA
                     CDDFIQEIRASLKMEDGRNATQVREYFDKIRPNAKKVVIVKQIAGQGAMYDNQLFSKE
                     PSGFEGGTSIIDMGNVPMIISPNEYRDGALRAMT"
     gene            6100..6567
                     /locus_tag="PC700_002568"
     CDS             6100..6567
                     /locus_tag="PC700_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HDF2581867.1"
                     /translation="MGIGPSTKETSLHHFRDPLLDIVESDKDVDLLGVIVVGTPDGNE
                     NKTFVGQRTAAWLEAMRVDGAIVSSDGWGNSHVDYANTFEEIGKRDIPVVGVTFNGTQ
                     AKFVVSNQYMDTIVDMNKSKEGIETEVVGENNTNEIDAKKALAFLKLKMRKHG"
     gene            6585..7067
                     /locus_tag="PC700_002569"
     CDS             6585..7067
                     /locus_tag="PC700_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HDF2581868.1"
                     /translation="MSFVGISTSMKNTSLHIFRDPLLDLIDNDSDIDLLGVVVVGTSE
                     HNEWKTFLATRLGRWIESLRPDGVIITLDMAGNQHIDFTNAIAEFVKSDIPAVGLTIM
                     GADGLVITNPYLDKATIIDYKKTTGYIETEVVGDNHMDEVDVKKALAFLKLKMRKDAK
                     "
     gene            7087..8094
                     /locus_tag="PC700_002570"
     CDS             7087..8094
                     /locus_tag="PC700_002570"
                     /EC_number="5.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase"
                     /protein_id="HDF2581869.1"
                     /translation="MKFSRSIQAIDSHTAGEATRIVVGGIPNIKGNSMPEKKEYLEEN
                     LDYLRTAIMLEPRGHNDMFGSVMTQPCCPDADFGIIFMDGGGYLNMCGHGTIGAMTAA
                     IETGVVPAVEPVTHVVMEAPAGIIRGDVTVVDGKAKEVSFLNVPAFLYKEGVEVDLPG
                     VGTVKFDISFGGSFFAIIHASQLGLKIEPQNAGKLTELAMKLRDIINEKIEIQHPTLA
                     HIKTVDLVEIYDEPTHPEATYKNVVIFGQGQVDRSPCGTGTSAKLATLHAKGELKVGE
                     KFVYESILGTLFKGEIVEETKVADFNAVVPKITGSAYITGFNHFVIDEEDPLKHGFIL
                     K"
     gene            8214..9095
                     /locus_tag="PC700_002571"
     CDS             8214..9095
                     /locus_tag="PC700_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HDF2581870.1"
                     /translation="MVAILKALYVVFAAIFAVVFGKDVAKANKEGKLEEQSTPKVAVT
                     GFITDFFDTLGIGSFAPTIAMSKALKLNIPDKKMPGTLNVAHTIPVVTEAFIFTSIIP
                     VDGVTLVSMVVAAAVGSYIGAGIIAKMDERKIQLVMGVTLALTAILMLLGHPWINVLP
                     GGGNELGLTGVKLVIGVIGNFILGALMTAGIGLYAPGMAMVYFLGMSAKVAFPIVMGS
                     CALLMPVASMKFIKEDAYTKKASVIIAICGLIGVFVAAYIVKTLPMDILKALVIVVIA
                     YTSATMIMAANKNKKTA"
BASE COUNT         3676 a         1150 c         1881 g         2672 t
ORIGIN      
        1 tatatataaa taagttttga tgaggtacta ctataaaggg ggggacttta tgttttcgat
       61 accagaagta aaaaaagtag aagaggtaat ggatacaaaa tttacaacta tagacgaaga
      121 cacaagaatt gaagatgcaa taaaagaaat gataaaaagc aacacaaaaa ctctaatggt
      181 aatagattca tctgaccaat taaaaggcat tatatccatg acggatatac ataatcttta
      241 tgagatgcat aaaaagtatg aaggtcaacc agtaaaactt ataatgaaga aggatgtaat
      301 atatgtcaac gaaggtttaa ccttagatga atgtagagat ataatgatat taaaaaatat
      361 agggattctt cctgttctta gagataataa aattattgga gttctaaaac aagagcacat
      421 tagggactat ctatatatgc atttagaaga ttatggattg actttgaagt atattatagg
      481 tcaaattaaa gaaggaattt gtgctataaa taacgaaggt gttgttatct tgtggaataa
      541 gtttatggag gaaaggtatg atataaagtc tgaagatata gttggtcgac cgatgaatga
      601 atttttggaa aatactattt cagaaaaagt actaaatagt aaagttggaa tgagtgactt
      661 gtattttaca gataaaaaag aaaatatgta tgcattagtt catgcaaatc ctatatttta
      721 taaagaagaa tttatcggtg ttgtatgtac agaagttgat gttacagaag ctaagatatt
      781 agctcttgaa cttgaaaaag taaatgatac attaaaatat ctaaagaacg aagtcaaaaa
      841 tttatctaaa ggtagttttg ataagatttt aggtaaaagt tataaattgg aaaaatcgaa
      901 agcgattgca aaacaagtgg ctagaacaaa tagtagcata tttatttggg gtgaaagtgg
      961 tacgggtaaa gaagtatttg caagagctat tcatgattat agtgagagaa aaggtcaatt
     1021 tatacctgtt aactgtagtg ccataccaaa tgagttattt gaaagtgagt tttttgggta
     1081 tgaatcaggt gcatttacag gagcaagtaa aaagggtcga ataggtatat ttgaacttgc
     1141 aaaagatgga actgtatttt tagatgaaat tgccgattta cctcttagta tgcaagcaaa
     1201 gttgcttaga gtattacagg aaaaagaaat aagaagagtt ggtggagata ccacaataaa
     1261 aataaatcct agaataatat cagctaccaa taaagatttg agtaaaatgg taaaggcaga
     1321 aaaatttaga gaagatttat actatagatt aaatgttgtt gaaataaaga ttcctccact
     1381 tagagagaga aaagaagaca taggattatt ggttcatagc tttttagagg agatatgtaa
     1441 acaaaataat aagcctgttc ttacaattag caaagatgta atagatatat ttcagaatta
     1501 tagatggaaa ggtaatatta gagaattaaa aaatacgatt gaaaatattg ttgtattgtc
     1561 acaaaattct aaaatagatg tagatgatgt accaagttat atgatggatt ctacaaataa
     1621 tagtactgaa gaagaagagt atcccttaga tttaacgaag gcaactcaaa aaattgaaat
     1681 aaagaatata accaaggcac ttaaaatgtc taatggaaat aaagctaaag cagctaagat
     1741 attaaatata cccagaacaa ctttgtatta taagatagac caatacaaaa tagatgtgtc
     1801 taaaatatga catatgacta aattttgaca aaatgataaa ctttaattta aaacctagta
     1861 aagccaacac ttgtataagt gttgtgtata tagatgtgtt aaaaaaaatg aaaaagtgtt
     1921 gattaattga cacttttttt gtttgagaaa atgtttacaa atacaaaaaa taacatattt
     1981 taaataaata tttatataat atttggctaa aaatagcgat agatgaagaa tctattatag
     2041 aaaaaatctt tttgttaata agttggcata ggaattgctt ataatataaa gtgactttga
     2101 gtgagaaaaa aataatatta atcataacaa attatgtagg ttttaattta cttaatttct
     2161 cagttaaggg gttacctgtt gaggaattga gatttaaaat aataaataag aaaataatgt
     2221 aaggaggtat tttatactat gtcaataact ttagaaacag ctcaagccca tgcaaatgac
     2281 ccagcagttt gttgttgtag atttgaagcg ggaacaatta tagcgccaga aaacttagaa
     2341 gatccagcaa tatttgcaga cttagaggat tctggattat taacaatacc agaaaatgga
     2401 ttaactatag gtcaagtact aggagctaag ttaaaagaaa ctttagatgc actttctcca
     2461 atgactacag ataacgtaga aggatacaaa gcaggagagg ctaaagaaga agtagtagaa
     2521 gaaacagtag aagaagcagc tccagtatca gaagcagcag tagttccagt aagcacagga
     2581 gttgcaggtg aaacagttaa aatacacata ggtgaaggta agaacataag cttagagata
     2641 cctttatcag tagctggtca agcaggagtt gctgctccag tagcaaacgt tgctgctcca
     2701 gtggcaagtg cagcagcaga agtagctcca aaagttgaag aaaagaaact tttaagaagc
     2761 ttaactaaaa aacactttaa aatagataaa gttgaatttg ctgatgaaac taaaatagaa
     2821 ggaactactt tatacatcag aaacgcagaa gaaatatgta aagaagctaa tgaaactcaa
     2881 gagttagttg tagatatgaa gttagaaata ataactcctg ataaatatga aacttacagt
     2941 gaagctgtat tagatataca accaatcgct actaaagaag aaggcgaatt aggttcaggt
     3001 ataactagag ttatagatgg agctgtaatg gtattaactg gtacagatga agatggagtt
     3061 caaataggtg aatttggttc ttcagaaggt gagttaaata ctactataat gtggggtaga
     3121 ccaggtgctg ctgacaaagg tgaaatattc atcaaaggtc aagtaacaat aaaagcagga
     3181 actaacatgg aaagaccagg acctttagct gctcaccgtg catttgacta tgtaactcaa
     3241 gaaataagag aagcattaaa gaaagttgac aactctttag tagttgatga agaagtaata
     3301 gagcaataca gaagagaagg taaaaagaaa gttgttgtta taaaagaaat aatgggacaa
     3361 ggtgcaatgc atgataacct aatattacca gttgagccag ttggtacatt aggagctcaa
     3421 ccaaacgttg acttaggaaa catgccagtt gtattatctc cacttgaagt attagatggt
     3481 ggtatccatg cattaacttg tataggacct gcatcaaaag aaatgtcaag acattactgg
     3541 agagagccat tagtaataag agctatggaa gacgaagaaa tagatttagt aggtgttgta
     3601 tttgttggtt ctccacaagt aaatgctgag aaattctatg tatctaagag attaggtatg
     3661 ttagttgaag ctatggaagt tgatggagct gtagtaacta ctgaaggttt cggaaacaac
     3721 catatagatt tcgcatctca catagagcaa ataggtatga gaggtatacc agtagttggt
     3781 gtaagtttct cagctgttca aggtgctcta gttgttggta ataaatacat gactcacatg
     3841 gtagacaaca ataagtctaa gcaaggtata gagaatgaaa tattatctaa caacacttta
     3901 gctccagaag atgctgttag aataatggct atgcttaaaa atgctataga aggtgtagaa
     3961 gttaaagctc ctgaaagaaa atggaatcca aatgttaaat taaataacat agaagctata
     4021 gaaaaagtta caggagaaaa aatagtatta gaagagaatg agcaatctct accaatgagt
     4081 aagaagagaa gagaaatata cgaaaaagac gaaaactaaa atttagatac taactaagat
     4141 ttagacaata acataaattt agatccaagt ttaaaacagt atagagaggt agaattataa
     4201 tggataggtt aaaatatatc tcatcagaga ctttttatga aggtattata gttgacatca
     4261 aaggtggtgg agtgactata gacctaaaag gtagacttgg acaatttaaa atacctaata
     4321 gaatgcttat aactgactac gaacttaaga taggtcaaga agttggattc atgctaagct
     4381 atcctgaggt attaagtcca gagcctaatc aagagtatgt agaaaatatt aggagagaaa
     4441 aagaaaaaca agcagaaatg caatcaaaac aagaagaaaa ataataaata atcgtttgaa
     4501 aatagaaggg agaggaaata tatgagcctt acaacagtac aaggacttca atctgaaata
     4561 ttcgttccaa taacacctcc tccagtttgg actcctgtaa ctaaagaatt aaaagatatg
     4621 actatagctt tagctacagc agctggtgtt catttaaaat ctgacaagag atttaatcta
     4681 gcaggagata ctacatttag agctatacct aatacagcta cagttgatga aatgatggta
     4741 tcacatggtg gatatgacaa tggagatgta aacaaagata taaactgtat gttccctata
     4801 gatagattac atgaattagc tgcagaagga tttatcaaag gagtagctcc tatgcactat
     4861 gcattcatgg gtggtggagg aaaccaacat gtcttcactg aagaaactgg tcctgctatc
     4921 gctgctaaac ttaaggaaga gggagtagac ggtgtagtta tgacagctgg ctgaggtact
     4981 tgccatagaa ctgccgtgat cgtgcagaga gcaatagagg aagctggaat acctacaata
     5041 ataatagcag ctcttcctcc agtagttaga caaaacggaa ctcctagagc agttgctcca
     5101 ttagttccaa tgggtgctaa tgctggtgaa ccacataata tagaaatgca aacacatata
     5161 ttaagagata cattagagca attagttgca ataccatctg ctggtaagat agttccatta
     5221 ccatacgaat ataaagctca cgtttaaaat gtaatttata tttgaaaata tagaaccctt
     5281 ctcctacacg gttaggagag gggttttaag aacaaaggtg gatattatgg aagaaaaaat
     5341 aatgagaaga cttgttataa aaccgtttca tatgaatgaa gttaatttcg gttcaaaaac
     5401 ttctataaag aaagatgtac ttacaataga tttatcaagt atagatgaaa taaaagaaag
     5461 agaagagtta ataacagaca taaaagtaga cattataaaa cctggagatt atgatagaga
     5521 aattaatact attatggaca ttataccaat atcaacaaaa gttttaggca gactgggaga
     5581 gggtatcaca catactttaa cgggtgtata tgtaatgtta actggtgcag atgaagatgg
     5641 aaatcaaatg catgaatttg gttcttcaga aggaattttg aaagaccaaa tggtatttgg
     5701 gagatatgga actccatcag ttgaagatta tataatccat gtagatgtaa ctttaaaggg
     5761 aggtttacct tttgaaagaa cacttccact tgcagcattc agagcatgtg atgattttat
     5821 acaagaaata agagcgtctt tgaaaatgga agacggaaga aatgctactc aagttcgtga
     5881 atattttgac aaaattcgac caaatgcaaa aaaggttgtc atagtaaaac aaatagcagg
     5941 tcaaggtgct atgtatgaca atcagttatt ctcaaaggaa cctagtggat ttgaaggagg
     6001 aacatcaatt atagatatgg gaaatgtacc tatgattata tctcctaatg aatacagaga
     6061 tggtgccctt agagctatga cttaagagag gtggatatta tgggaatagg accttcaaca
     6121 aaagaaacat ctcttcatca ctttagagac ccacttttag acatagttga aagtgataag
     6181 gatgtagatt tacttggagt aatagtagta ggaactccag atggaaatga aaataaaacc
     6241 tttgtgggac aaagaacggc cgcttggctg gaagccatga gagtagatgg agctatagtt
     6301 tcatcagatg gttggggaaa ctcacatgtt gactacgcta acacatttga ggaaatagga
     6361 aaaagagata tacctgtagt tggagttaca tttaatggga ctcaagctaa gtttgttgta
     6421 agcaatcaat atatggatac catagttgat atgaacaaat caaaggaagg tattgagaca
     6481 gaagtagttg gagaaaacaa cacaaatgaa atagatgcta aaaaagcttt agcattttta
     6541 aaacttaaaa tgagaaaaca tggataaaaa atttaagtaa gggaatgagt tttgtgggaa
     6601 tttctacatc aatgaaaaac acatcacttc atatatttag agacccgtta ctagatttga
     6661 tagataatga ttctgacata gacttgttag gagtggttgt agttgggaca tcagagcaca
     6721 atgaatggaa aacattttta gcaactagac ttggaagatg gattgaatca ttgcgacctg
     6781 atggagtgat tataacttta gatatggcag gaaaccagca cattgatttt acaaatgcta
     6841 tagcagagtt tgtaaaatca gatatacctg cagtaggtct tactattatg ggagcagatg
     6901 gattagtaat taccaatcca tatttggata aagctaccat aattgattat aaaaagacaa
     6961 caggctacat agaaacagaa gtagttggag acaatcacat ggatgaagtt gatgttaaaa
     7021 aagcattggc tttcctaaaa ttaaaaatgc gtaaagacgc aaaataaacc taagggggaa
     7081 tttgatatga aatttagcag aagtatacaa gctatagact ctcatacagc aggtgaagca
     7141 actagaatag ttgtaggtgg aatacctaac ataaaaggaa attctatgcc tgagaaaaaa
     7201 gaatatttag aggaaaatct agattattta agaactgcta taatgttaga gccaagagga
     7261 cataatgata tgtttggttc tgtaatgact caaccttgtt gcccagatgc tgactttgga
     7321 ataatcttca tggatggtgg tggatacctt aacatgtgtg gccatggtac aataggagct
     7381 atgacagcag ctatagaaac aggtgtagtt ccagcagtag agcctgtaac tcatgtagtt
     7441 atggaagctc cagcaggaat aataagagga gacgttacag ttgtagatgg aaaagctaaa
     7501 gaagtatcat tcttaaacgt accagctttc ttatataaag aaggtgttga agttgactta
     7561 ccaggtgttg ggactgttaa atttgacata tcatttggtg gaagcttctt tgctataata
     7621 catgcaagtc aattaggttt aaaaatcgaa cctcaaaatg ctggtaaatt aactgaacta
     7681 gctatgaaac ttagagatat aataaatgag aaaatagaaa tacaacatcc aactttagct
     7741 catataaaaa ctgtagactt agttgaaata tatgatgagc caactcatcc agaagctact
     7801 tacaaaaatg tagttatatt tggtcaaggt caagttgaca gatctccatg tggaactgga
     7861 acaagtgcta aattagctac tttacatgct aaaggtgaat taaaagtagg agaaaaattc
     7921 gtatatgaaa gtatattagg aactttattc aaaggtgaaa tagttgaaga aactaaagtt
     7981 gcagatttca atgctgtagt acctaaaata actggttctg cttatataac tggatttaat
     8041 cattttgtaa tagatgaaga agacccactt aaacatggat ttattcttaa ataagtaata
     8101 aataaattag gaaattaaaa tagaatagta gccacttgtc ggttgctatt ctatagcttt
     8161 taggctataa gattcagaat atatgaaaaa ataaaaaaat aggaggatgg attatggtag
     8221 caatcttaaa ggcattatat gtagtattcg cagcaatctt tgcagttgtg tttggtaaag
     8281 acgtagctaa agcaaacaaa gaggggaaac tagaagaaca aagtactcca aaagttgctg
     8341 taacaggatt tataacagac ttctttgata cattaggaat agggtcattt gctccaacaa
     8401 tagcaatgtc aaaggcttta aagttaaata taccagataa aaaaatgcct ggtacattaa
     8461 acgtagcaca tactattcca gttgttacag aagcattcat atttacatca atcataccag
     8521 ttgatggtgt tactttagta tctatggttg tggcagcagc agttggttca tatataggtg
     8581 caggaataat agctaagatg gacgagagaa aaatacaact tgttatgggt gtaactttag
     8641 cattaacagc aatattaatg ttattaggtc atccttggat taacgtatta ccaggtggag
     8701 gaaatgaatt aggtcttact ggtgttaaat tggtaatagg tgtaataggt aactttatac
     8761 taggagcttt aatgacagct ggtataggtc tatatgctcc aggtatggca atggtttatt
     8821 tcttaggaat gtctgcaaaa gttgcattcc caatagttat gggttcttgt gcattattaa
     8881 tgccagttgc aagtatgaaa tttataaaag aagatgcata tactaaaaaa gcatcagtta
     8941 taatagctat atgtggttta ataggagttt tcgtagctgc atatatagta aaaactctac
     9001 caatggatat attaaaagca ttagtaatag tagttatagc ttatacttct gctacaatga
     9061 taatggctgc aaataaaaac aaaaagactg cttagttaaa gactacttag tgtttattaa
     9121 atattacagc taataataaa gattataaac tgctttaaaa tattgaatta atattttagg
     9181 gcagtttttt atttgctaaa ttaatataaa gcaattaaca tgccaattat gtaatatata
     9241 tagtgattta gtagatttaa tgtaaaaaac gttgtatttt caaagaataa agcataattt
     9301 aaagctaaat atttaaattt tataatcaat aagttcaata aatgttcata tattaataca
     9361 gattaataaa aatataagc
//
LOCUS       DAMJWP010000138         9307 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.143, whole genome shotgun sequence.
ACCESSION   DAMJWP010000138 DAMJWP010000000
VERSION     DAMJWP010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9307
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.143"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..315
                     /locus_tag="PC700_002572"
     CDS             <1..315
                     /locus_tag="PC700_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2581871.1"
                     /translation="EDLQVKNMIKNRKLSRLISDVSWSEFVRQLEYKANWHGRQIVKV
                     GKFFASSQICNKCGYKNEEVKNLNIREWICPSCNETHDRDINASINILKEGLRLITIQ
                     NK"
     gene            458..1006
                     /locus_tag="PC700_002573"
     CDS             458..1006
                     /locus_tag="PC700_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2581872.1"
                     /translation="MGGVDSVLSSVKVKNIIQANYKKDSKQYDEYIDSLKKADIEPVL
                     LKDNMNIVINSAEINIHPASKSKYESSNDYSIITNISYGAHKFLFAGDAEEERLSEFI
                     NGNTLKYDFVKMPHHGRYDKLTETFLESTSPQYAVITCSEKKEPEEGVLKILERLNIK
                     TFLTSSGEVVINSDGKTLSVNQ"
     gene            1125..1316
                     /locus_tag="PC700_002574"
     CDS             1125..1316
                     /locus_tag="PC700_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581873.1"
                     /translation="MLDRFFGEGGFGGCAWWIIILFFLFLAFQDCWAEMDLCAWIPFL
                     ILLLILCECGDVFDGGCGC"
     gene            1539..1886
                     /locus_tag="PC700_002575"
     CDS             1539..1886
                     /locus_tag="PC700_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581874.1"
                     /translation="MAIVDKIFNEIKLMVARLLLGKKYSEYEEKDLVYNTEEVILITL
                     KRLVFQGNVNEAEEILFDKAKTVTSENMQYIAIEFYTMLMEKTDEELEAMNFSKQEVY
                     QGIEDIRKVLNLE"
     gene            2148..3653
                     /locus_tag="PC700_002576"
     CDS             2148..3653
                     /locus_tag="PC700_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HDF2581875.1"
                     /translation="MKVGFDHKKYLEEQSKYILERVNNFDKLYLEFGGKLMGDLHAKR
                     VLPGFDENAKIKVLQHMKEKVEVVICVYAGDIERNKIRGDFGITYDMEVLRLIDDLRG
                     YELDVNSVVITRFEGQPATTVFINKLERRGIKVYKHYPTKGYPSDVETIVSDEGYGAN
                     EYIETTKPIVVVTAPGPNSGKLGTCLSQLYHENKRGNVVGYSKFETFPVWNVPVKHPL
                     NIAYEAATVDLKDVNMIDSFHLETYGEMSVNYNRDLELFPVLKKIIEKITGKESIFQS
                     PTDMGVNRVGFGIIDDEVIRKASIEEIIRRYFKTACEYKKGQVDKGAYDRMKMIMEEL
                     NLKPEDRNVVLPARNYSNKLKESADKNDTCPVVALELEDGTILTGKGSDLMNGTAAVV
                     LNAIKHLANISDDMHLISPVILEPIINLKTKTLASRNVSLSCQEVLTALSICAVTNPT
                     AQFAMEKLALLKGCQAHSTTILNRDDEQTFRKLGIDVTCDPEYPSQNLYYS"
     gene            4043..5140
                     /gene="dinB"
                     /locus_tag="PC700_002577"
     CDS             4043..5140
                     /gene="dinB"
                     /locus_tag="PC700_002577"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDF2581876.1"
                     /translation="MKHYRKIIHIDMDAFYASIEQRDNKKLKGKPVIVGGNPQSRGVV
                     ATCSYEARKFGIHSAMPSAVAYNRCPYAVFVRPRFDVYKSVSEQIRDIFYRYTDLVEP
                     LSLDEAYLDVTKNKKNIDSSIEIAKQIKKDIFREVGLTSSAGVSYNKFLAKIASDLRK
                     PNGLTVITEENAQDFLDKLPVNKFFGVGKVTSNTLKNLGIKTGYDLRCLNLFELENIF
                     KKRGYELYKFARGIDDRPVEPNRVRKSVGAETTLSHNLDIDEEETRNILDELCEEVCH
                     RLKSSEKFGKTLTLKIKYEDFTKITRSLSLEHYIDEYNDIRSGVDNLLRNVEVNGKQI
                     RLLGVTISNLSDKKETYKDITLFEYMDSIQM"
     gene            5396..6532
                     /locus_tag="PC700_002578"
     CDS             5396..6532
                     /locus_tag="PC700_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2581877.1"
                     /translation="MIEIRNVTKKIGNNVILDDISLVVETGTLVVLIGSSGCGKTTTL
                     KLINKLIKPTSGEIYINGKPISQENEIELRRKIGYVIQNTGLFPHLTIKENIELIPRL
                     KKEKSVEEIEKRTLQLLEMVGLDSDEFLNKYPSELSGGQQQRIGVARAIATDAEIILM
                     DEPFSALDPITRTSLQEQLFSLQDELKKTIIFVTHDMDEALKIADKICIMKDGRIAQY
                     DTPENILRKPANDFVKDFIGEDRVWDNPEYIKARDIMIKNPIAVNSTRTVTQGIEIMR
                     TSKVDSLLIIDRAKTLKGIVTVKDMKDIDDKSILLADIMSSEPLHVNEGDNLVEILNV
                     MNRNSVGYIPVISDENKLVGLITRSSLLSVLSEQFLEMEVSVLG"
     gene            6525..8072
                     /locus_tag="PC700_002579"
     CDS             6525..8072
                     /locus_tag="PC700_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDF2581878.1"
                     /translation="MANFFNFILLQKDKIIELLIQHMSLTVTSILIAIIVGVPLGIII
                     SRISSLRKFVLGFVNLVQAVPSMALLGLLVPILGIGSKPAIFMVVVYSLLPIVKNTYI
                     GITSIDPVVLESAKGIGLTRNQTLFKIQFPLALPIIMGGVRISAVTAVGLMTLAAFIG
                     AGGLGYLVFSGVQTVNNNMILAGAIPSCIIALLVDYLFSKIEVAVTPKGLNPKAPKKN
                     YIALKVISVVVVVSMLFVVFSSSFSSKKDTITIGSKDYTEQLILGNVYAELVEKNTNL
                     KVKKNLNLGGSSVAFNAIKSGELDMYVDYTGTLLVNVMKHAPIKDADEAYNVVKDTME
                     KENQLTLLDPLGFNNTYTLAMMPETAEKYGINTISDLTKYGKEFTFSPTLEFENREDG
                     LVGLSRDYGLKFKDVKAMNGSLRYTALDNNESQVIDAFLTDGLIKKFNLKILEDDKNF
                     FVPYYAAPLVREDTLKKYPELEKVMNMLSGKINEETMRELNYQVDELGKSPEEVAHSF
                     LVKEGLV"
     gene            8406..9272
                     /locus_tag="PC700_002580"
     CDS             8406..9272
                     /locus_tag="PC700_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDF2581879.1"
                     /translation="MDNYKKVKQVLWIILFANFAVALLKIIIGNQIKSYSMTADGFHS
                     LSDGASNIVGLIGIFFASKPKDKNHPYGHKKFEIITSLFISGMLFVIAIKIILSAVLR
                     IANPVVPAITIESLIALIITLFINIFVCMYEYRIGTKLNSYVLISDSLHTRSDIFVSL
                     GVLVTLVGVKLGFPVIIESIVPIIISAFIIYSAYGIFRPSIGILVDRVAVDEDYIKEI
                     VFEFNEVRDVHNIRSRGSKSSIYIDMHVMVDPFISVEQSHDLTHKIEKQIQEEINENA
                     QVIVHIEPFYSF"
BASE COUNT         3582 a          995 c         1656 g         3074 t
ORIGIN      
        1 gaagatttac aagtaaagaa tatgattaaa aatcgtaaac tatctcgctt aatttctgat
       61 gtgtcatggt cagaatttgt tcgtcaatta gaatacaagg ctaattggca tggtagacaa
      121 attgtaaaag taggcaaatt ctttgcaagt tcacaaatat gcaataaatg tgggtataaa
      181 aatgaggaag ttaagaactt aaatataaga gaatggattt gtcctagttg taatgaaact
      241 cacgatagag atataaatgc aagtataaat atattaaaag aaggattaag actaataaca
      301 attcaaaata aataacgtat gtaagaaccg taggaactac ggggatagcc tgtggagaat
      361 tagtaagaca tatgttagta tgcaaaattc tatgaagcag gaaccctgtg actttagtca
      421 taggaggttc agtttaaccc atttagataa ggaccatata ggtggagtag atagtgtgtt
      481 atctagtgtt aaagtaaaga atattataca agcaaattat aagaaagata gtaagcaata
      541 tgatgaatat attgactcac ttaaaaaagc tgatatagaa ccagtgctac taaaagataa
      601 tatgaatatt gtaatcaata gtgcagaaat aaacatacat ccggcttcaa aaagcaagta
      661 cgagagttca aatgattatt caataatcac taatataagc tatggagcac ataagttttt
      721 atttgcagga gatgcagagg aagaaagatt gtcagagttt ataaatggaa atactttaaa
      781 atatgatttt gttaaaatgc ctcatcatgg aagatatgac aaattaactg aaacattttt
      841 agagtctact tcaccccaat atgctgttat tacatgttca gagaaaaaag aaccagagga
      901 aggtgtttta aaaatattgg aaagattgaa tataaaaaca tttttgactt ctagtggaga
      961 ggtagttatc aatagtgatg gtaagacatt gagtgtgaat caataatttt ttttaaaata
     1021 tctaaaaaaa atttaaaaaa atcattagca aatattaccc cttaaacata gtatatttta
     1081 ggagctgaaa ctccacatac aaaatgacta ggaggtaaca tattatgttg gatagatttt
     1141 ttggtgaagg cggatttggt ggatgtgctt ggtggataat aatattattc ttcttattct
     1201 tagcattcca agattgttgg gctgaaatgg acttatgtgc atggatacct ttcttaatat
     1261 tattattaat actttgtgaa tgtggtgacg tttttgatgg aggatgtgga tgctaaaaaa
     1321 atcaatttag atgatttaat aaagcttatg gtgggtataa ataaaatacc aaaccaatca
     1381 caatctagtg tttgaaggta gtcggaaatc ccccgctacc ttcttttttt tattgttaga
     1441 atttattttt gaattatttc atttacatat aataattttt acaataaagt ctgatataat
     1501 taaaaagaaa ataaaattta tttataggag atgtaaaaat ggctatagtg gataagattt
     1561 tcaatgagat aaaacttatg gttgcaagac ttttattggg aaaaaaatac agtgaatatg
     1621 aagaaaaaga tttagtgtat aatacagaag aagttatact tataacttta aaaagattag
     1681 tttttcaggg aaatgtaaac gaagcagaag aaattttatt tgataaagct aagactgtaa
     1741 ctagtgaaaa tatgcagtat atagcaatag aattttatac tatgcttatg gaaaagactg
     1801 atgaagaatt agaagctatg aatttttcta aacaagaagt ttatcaaggt atagaagata
     1861 ttagaaaagt attgaattta gaataaaaga tatatgaaaa tattgaaata aattaatgtt
     1921 aaggtgagaa tattatactt atatataaaa atataatatt cttattttat tagttatatt
     1981 aggtatatct tatttaccat aaaactatct tgaaaaaaat taatcttaat tgtataatat
     2041 tactttgagt gaaaaagtat taacattttt ttataagatg ttttactaaa aaaatatata
     2101 taaatatact gttatcaaca agaaatttaa tttagtgagg tgttaagatg aaagtaggat
     2161 ttgaccacaa aaaatattta gaagagcagt ctaaatacat cttagaaaga gtaaataatt
     2221 ttgacaaact ttatttagag tttggaggaa aattgatggg agaccttcat gcaaaaagag
     2281 tacttccagg atttgatgaa aatgcaaaga taaaagtatt acaacatatg aaagaaaaag
     2341 ttgaagttgt aatttgtgtc tatgcaggag atatagaaag aaacaaaata agaggagact
     2401 ttggtatcac ttatgatatg gaagttttaa gattaataga tgatttaaga ggttatgaac
     2461 tagatgtaaa tagtgtagta attactagat ttgaaggaca acctgctaca actgtattta
     2521 taaataaatt agaacgtaga ggaattaaag tttataagca ttatccaaca aagggatatc
     2581 cgtcagatgt tgagactata gttagtgatg agggatatgg agcaaatgaa tatatagaaa
     2641 ctacaaagcc aatagtagta gtcacagcac caggacctaa tagtggaaaa cttggtactt
     2701 gtctaagtca gttgtatcat gaaaataaaa gaggtaatgt agttggatat tctaagtttg
     2761 agacattccc agtttggaat gtacctgtaa aacatccttt gaatatagct tatgaagcag
     2821 ctacagttga tttaaaagat gtgaatatga tagattcatt ccaccttgaa acgtatggtg
     2881 aaatgtcagt aaactacaat agagatttgg aattatttcc agtgcttaaa aaaataatag
     2941 aaaagataac tggaaaagaa tcaattttcc aatctccaac tgatatgggt gttaatagag
     3001 ttggctttgg aataatagat gatgaagtaa taagaaaagc ttctatagaa gaaataataa
     3061 gaagatactt taaaactgcc tgtgagtata aaaagggaca agttgataaa ggcgcttatg
     3121 atagaatgaa aatgattatg gaagagctta acttaaagcc agaagataga aatgttgtat
     3181 tacctgctag aaattacagt aataaattaa aagaaagtgc cgacaagaat gatacttgtc
     3241 ctgtagttgc attagaatta gaagatggta caatattaac tggtaagggt tctgatttaa
     3301 tgaatggaac agcagcagta gtattaaatg ccattaaaca tttagccaat atatctgatg
     3361 atatgcactt aatatcacct gtaatattag aacctatcat aaacttaaaa acaaagactt
     3421 tagctagtag aaatgtttca cttagttgcc aagaggtgtt aactgcactt agtatttgtg
     3481 ctgtaactaa tccaactgca cagtttgcta tggaaaagtt agcattatta aaagggtgtc
     3541 aagctcattc tactactata ttaaatagag atgatgaaca aacatttaga aagcttggta
     3601 tagatgtaac ttgtgaccca gaatatcctt cacaaaatct ttactatagt taaaattata
     3661 taaaaaaata tgggctgttt taaagtaata gtttgagcag tccattacaa ttataataaa
     3721 atcagtaggc tgtcttgtat aagacagtct tttgttttta aaacatccca taaattaaat
     3781 atatatactg ttatccataa ggaattttaa atttagttcc ttggaatacc tattgtaaag
     3841 cttttatata gtttttgcaa gtgtttttaa ggatttttta taaattatat tgaagttaat
     3901 ggattataga tggttttatt tatggaaaat atgaatatat aaagaaaata atgaaaaaat
     3961 accaaaaaga aacataagtt tggtataatt aaaaagtaat ataaataaaa ttaatctatt
     4021 tatactttaa aaggtgataa taatgaaaca ttatagaaaa ataattcata tagatatgga
     4081 cgctttttat gcatctatag aacaaagaga taataaaaaa ttaaaaggaa aacctgttat
     4141 tgtcggtgga aatccacaaa gccgaggtgt ggttgcaacg tgttcttatg aagctagaaa
     4201 atttggtatc cattcagcta tgccatcagc tgtagcttac aataggtgtc catatgcagt
     4261 ttttgtaaga cctagatttg atgtttataa gagtgtgtca gagcaaataa gagatatatt
     4321 ttatagatac acggatttag ttgaaccatt atctttagat gaagcttact tagatgtaac
     4381 aaaaaataaa aaaaatattg attcatcaat tgagatagca aagcaaataa aaaaagatat
     4441 atttagagaa gttggtctta cttcttcagc tggtgtgtca tacaataagt ttcttgctaa
     4501 gattgcatca gacttaagaa agccaaatgg gttaactgta ataaccgaag aaaatgccca
     4561 ggacttttta gataaattac ctgtgaataa attttttggt gttggaaaag ttacttcaaa
     4621 tactcttaaa aatttaggta taaaaactgg atatgattta aggtgtttaa atttatttga
     4681 acttgaaaat atattcaaaa aaagaggtta tgaattatat aaatttgcta gaggaataga
     4741 cgatagacca gtggaaccaa atagggttag aaaatctgtt ggggctgaga ctacattaag
     4801 tcataatttg gatattgatg aagaagaaac acgcaacatt cttgatgaac tttgtgaaga
     4861 agtatgtcat agattaaaaa gctctgagaa gtttggaaaa acattaacat taaaaattaa
     4921 atatgaggac tttacaaaga taactagaag ccttagttta gaacactata ttgacgaata
     4981 taatgatata agaagtggtg tagataattt gttgagaaat gttgaggtta atggaaaaca
     5041 aattaggcta ttgggagtta caatatcaaa cttaagtgat aaaaaagaaa cttataaaga
     5101 tattacatta tttgaataca tggatagtat tcaaatgtga gttttgttta aatgaaataa
     5161 cactagcttt aatactatat aattgaggct agtgttattt tatgaatatt tttatacttt
     5221 ttgttttttg acattatata ctatatataa atactttata tttgtagcaa tattcagaca
     5281 aatagtgacc taaaaatcgt actttgttta cataaataaa atagtatgat ataattataa
     5341 aaacaactta taatattgga gaaggttgct tgttcgaaaa aggggatgat aaattatgat
     5401 agagataaga aatgtaacaa aaaaaatagg aaataatgtt atattagatg atatttctct
     5461 agttgtagaa acaggtacat tggtagtttt gattggttca agtggttgtg gaaagactac
     5521 tactttgaag cttataaaca aattaataaa acctacatct ggtgagatat atattaatgg
     5581 caaaccaatt tctcaggaaa atgaaataga gcttagaaga aaaataggat atgttataca
     5641 gaacacaggc ctattccctc atcttacaat aaaagaaaat atagagctaa ttccacgcct
     5701 aaaaaaggaa aaatccgttg aagaaattga aaaaagaacg ttgcaactac ttgagatggt
     5761 aggacttgat agtgatgaat ttttgaataa gtatccttca gaacttagtg gtggacagca
     5821 acaaagaatt ggtgttgcaa gagccattgc tactgatgct gaaataatat tgatggatga
     5881 gccttttagt gctttagacc caataacgag aacatcttta caagaacaat tattttctct
     5941 tcaagatgaa ttgaaaaaaa ctataatatt tgtaacgcat gatatggatg aggctttaaa
     6001 gatagctgat aaaatttgta ttatgaagga tggaagaata gcacaatatg atactcccga
     6061 aaatatactt agaaagcctg caaatgactt tgtaaaagat tttatcggag aagatagagt
     6121 ttgggataat ccagaatata taaaagcaag agatattatg ataaaaaatc ctattgctgt
     6181 taattccact agaactgtaa ctcaaggaat agaaattatg agaacgagta aggttgatag
     6241 cttactaatt atagatagag caaaaacatt aaaaggtatt gttactgtaa aagatatgaa
     6301 agatattgat gataagtcaa tattacttgc tgatattatg tcgagtgagc ctttgcatgt
     6361 aaatgaaggt gataatttag tagaaatact aaatgtaatg aatcgtaatt cggttggata
     6421 tattccggtt atttctgatg aaaataaact ggtagggctt attactagaa gtagtttact
     6481 ttctgtttta agtgaacaat tcttagaaat ggaggtgagt gttcttggct aattttttta
     6541 actttatact attacaaaaa gataagatta tagagctttt aatacaacat atgagtctaa
     6601 ctgttacatc tattttaata gctataatag ttggtgttcc tcttggaata attatttcaa
     6661 gaatctcatc tcttagaaag tttgtacttg gatttgtaaa cttagtccaa gcagttccgt
     6721 ctatggcact tttgggtctt cttgtaccta tacttggcat aggaagtaaa ccagccatat
     6781 ttatggtagt ggtttattca ttactaccaa ttgtaaaaaa tacgtatata ggtataacaa
     6841 gtattgaccc agtagtatta gaatcagcta aaggtattgg tcttacaaga aatcagacat
     6901 tatttaaaat acaattccct ttagcacttc ctataataat gggtggagtt agaatatctg
     6961 cagttacagc tgttggtttg atgactcttg cagcatttat aggtgctggt ggtttaggat
     7021 atttagtatt ttcaggggtt caaactgtaa ataataatat gattttagca ggggcaatcc
     7081 catcatgtat aattgcactg ttagttgatt atttatttag taaaattgaa gttgcagtta
     7141 ctccaaaagg attaaatcca aaagcaccta agaaaaatta tatagcactt aaagtaatat
     7201 ctgtagtggt tgttgtttca atgttgttcg ttgtttttag ttctagtttt tcaagtaaaa
     7261 aagatacaat aactattggt tctaaagatt atacagaaca acttatactt ggtaatgttt
     7321 atgctgaatt agtagaaaaa aatacgaatc ttaaagttaa gaagaattta aatctaggtg
     7381 gttcatctgt tgcgtttaat gcaattaaat ctggtgagtt agacatgtat gttgactata
     7441 caggtacact tttggtaaat gtaatgaaac atgctccgat taaagatgct gatgaagcat
     7501 ataatgttgt aaaagatact atggaaaaag aaaatcagtt aactttactt gaccctcttg
     7561 gatttaataa tacttacaca ttagctatga tgcctgaaac tgctgaaaag tatggaataa
     7621 atactatttc tgatttgact aaatatggta aagaatttac attctcacca actttggagt
     7681 ttgaaaatag agaagatggt cttgtagggc tttctagaga ttatggttta aaattcaaag
     7741 atgtaaaagc aatgaatgga agtttaagat atacagcttt agataataat gaatctcagg
     7801 ttatagatgc atttttaact gatggtctta ttaaaaagtt taatcttaag atattagagg
     7861 acgataaaaa tttctttgta ccatattatg cagcaccatt agtaagagaa gatactttga
     7921 agaaatatcc tgagttagaa aaagttatga atatgctttc aggaaaaata aatgaagaaa
     7981 ctatgagaga acttaactat caagtggatg aacttggtaa atcaccagaa gaagttgctc
     8041 attcattctt agttaaagaa ggcttagtat aaaaaattaa tagtttatat caaaaattta
     8101 agggagtgtc tcaaaatgaa ctttttagtt catgaggcac tcttttttat atgaaatcaa
     8161 atatatttta tcatttcaac aataaaaaag aagcttatct ttattttgag acagtctctt
     8221 atttttatgt ataccaaaac catacctacg atttttattt ataattaagt ttcaaataga
     8281 atgaacaagt acaaccaatt ttgaatataa atatagtaaa tgtgcttata tacgatttta
     8341 ttatctgggt gattgatatt gatagatagc tttctttatt gaatcgaagg gagggggaat
     8401 ttgttatgga taactataag aaagtgaagc aagtattgtg gataatttta tttgctaatt
     8461 ttgcagtagc attattaaaa atcattattg gtaatcaaat aaaaagctat agtatgacag
     8521 cagacggatt tcattctttg tcagatggag catctaatat agtggggctt ataggtattt
     8581 tctttgcatc taaaccaaaa gataaaaatc atccttatgg acataaaaag tttgaaatta
     8641 taaccagttt atttataagt ggtatgttgt ttgttatagc aattaagata attcttagtg
     8701 cagtgctaag aatagctaat ccagttgtac ctgctataac tatagaaagt ttaattgctt
     8761 taattattac actttttata aatatttttg tatgcatgta tgaatataga attgggacta
     8821 aactaaatag ttatgtttta atttcggatt cattgcatac aagaagtgat atatttgtgt
     8881 ctctaggtgt attggttact ttagttggtg ttaagttagg atttccagta ataatagaat
     8941 ctatagttcc tattattatt tctgcattca taatttattc ggcttatgga atatttaggc
     9001 catctattgg aattttggtc gatagagtgg ctgtagatga ggattatata aaagaaattg
     9061 tgtttgaatt taatgaggta agggatgttc acaatatcag aagtagaggt agcaaaagtt
     9121 ctatttatat tgacatgcat gtaatggtag acccatttat aagcgtagag caatctcatg
     9181 atttaacaca taaaattgag aaacaaatac aagaagaaat taatgaaaat gctcaagtaa
     9241 tagtccatat tgaacctttt tatagttttt aacttagcta taaaatacaa gttataaaaa
     9301 aatataa
//
LOCUS       DAMJWP010000139         9300 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.144, whole genome shotgun sequence.
ACCESSION   DAMJWP010000139 DAMJWP010000000
VERSION     DAMJWP010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9300
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.144"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            683..1522
                     /locus_tag="PC700_002581"
     CDS             683..1522
                     /locus_tag="PC700_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581880.1"
                     /translation="MFGYVKINKMDLTFREYDYYKAYYCGLCKYLKRNHGEISRFSLN
                     YDITFLIVLLTAVYNPESISTEEVCIVNPFKKKKVITNDITEYAASMNILLTYYKLED
                     NLMDDKRIKDKLAYYIYKNKLKLAYEKYPEKAEYIKQQLNELNKLEKDKNINIDEVSS
                     IFGNIMGEVFVYKKDENERNLRMIGFNIGKYIYLLDAYEDLDEDFKKGRYNPFIEYID
                     KNDELKEKVKKIIVTSLGFLARGIDNLNLNKNVGIIENIIYSGVYLRYINILESRGGK
                     NVQ"
     gene            1512..1955
                     /locus_tag="PC700_002582"
     CDS             1512..1955
                     /locus_tag="PC700_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581881.1"
                     /translation="MYSNTYNNENFMRARAFIENSEFKKAYDFLKTLTDKCAEWYYLT
                     GFSAMNIGYYEEGEDFLKRAKFMEPENSEYSDALRSYTQYRNDYSNRADNYNRRRRND
                     LDGCCCCCCDDCCCCLGDDCCENCAKLWCLDSCCECFGGDLITCC"
     gene            1966..2457
                     /locus_tag="PC700_002583"
     CDS             1966..2457
                     /locus_tag="PC700_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581882.1"
                     /translation="MSIKVAYGGVLLALNVILLTLTNIIPVNTLFIMGLASLLVSIVI
                     MEWGFKSGIAFYIGSIVLGFIVMASKSQWIVYSLTFGIYGIVKYLIEKDRSIYIEYFM
                     KLVFANIMILILYFLLRTIVYIPINIFIIGSFEIAFIVYDYVYSSFIGYYNNRLRKML
                     FKK"
     gene            complement(2659..3387)
                     /locus_tag="PC700_002584"
     CDS             complement(2659..3387)
                     /locus_tag="PC700_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HDF2581883.1"
                     /translation="MKNIAAFFDIDGTLYRDSLMVEHFKKLIKYDIIDQKNWYKHARD
                     TFMDWDKRQGNYDDYLLEICDLYVDSLKGLDKTCINFTSDQVIKLKADRVYKYTRSRI
                     EWHLNQGHIVIFISGSPGFLVEKMAKKYNVTDFLGSDYVFENNIFTGTVIPMWDSISK
                     NNAINDFVVKYDLDLSNSYAYGDTNGDINMLKRVGNPIAINPTKELLSQISLDEEISK
                     NVEIIVERKDIIYSLSTDVNIVDI"
     gene            3512..4600
                     /locus_tag="PC700_002585"
     CDS             3512..4600
                     /locus_tag="PC700_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581884.1"
                     /translation="MNFNFAKATNSRLMGSLGLIINWIDDENNHFCQYFLLDAEGLGL
                     ADYVSLNNPTQEEAYMEEERLMGGFGSDRVELTKDESLFLVSYFGNKNLYYDKLLPGD
                     KCEYIDIIKNYKTDLIIEKLYNKICKRVDEEVEFINYMTMRFIAWDRESLKYFSGSDE
                     IANMHITNINGTLLKNVVSDKGQGRYISEALYEDNDGYYICKIAFCISKCYETGFRIN
                     SLLVTDKELMYDFEVFDEISKSEFVSVYSVNSPEEFAGVFYRENPFLLKSNMNEGIFF
                     TRFNFNNDHVKENVYIINNDMKAIYYLMGNKFFIGTYNENDCNYINEISQSNYSDYIK
                     FEEKFFFEQNALYDFAESGSLDFDDFLE"
     gene            complement(5038..5886)
                     /locus_tag="PC700_002586"
     CDS             complement(5038..5886)
                     /locus_tag="PC700_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581885.1"
                     /translation="MSNKKKKDLDTSYMPYLDPANYADYITNEEIPQDELRNDPQISN
                     GYTKIPNNQNINNLYPNNVNHYPKSSNSPNNLPNTNNTSGNMNYNTSATNNMPNNMNN
                     NTGMPNNMSNNMNSNMGMPNNMPNNMNSNMGMPNNMSNNMNSNMGMPNNMPNNMNSNM
                     GMPNNMPNNMNSNMGMPNNMPNNMNSNMGMPNNVPNNMNSNMGYNMNNTMGIPNFSCN
                     QNMPPNVLMMIPGVICHNTMQGMPVVMPSTMPPNIYPTPYGSSNMSIQGIPQATNIEE
                     FDEEEM"
     gene            6085..6450
                     /locus_tag="PC700_002587"
     CDS             6085..6450
                     /locus_tag="PC700_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2581886.1"
                     /translation="MREEINDCNCNIVHEEIVTEAKSTMPDEEMLYDLAELFKVFGDT
                     TRVKILYALFANEMCVCDIASLLNMTHSAISHQLRVLKQARLVKFRREGKTVYYSLDD
                     SHISQIFDCGLNHIRETYK"
     gene            6473..8860
                     /gene="cadA"
                     /locus_tag="PC700_002588"
     CDS             6473..8860
                     /gene="cadA"
                     /locus_tag="PC700_002588"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDF2581887.1"
                     /translation="MEANNLIKKEFILGGLNCAHCAEEINNKVSKLQEVKSSNLNFIN
                     KKLIVNIKESFNEDTTIEKIIDIIDSTEPGLDIQISSKENASSKTSIKKELILGGLNC
                     AHCAEEINNKVSKLKEVESSNLNFVNKKLTVNISNNFKEDDVINKIKEIINSTEPGLD
                     IQVGSTDKVKGRTTEKSGAVNDTNKKELVPLIIGALVYIFGIYQTATGYESQFSNIVF
                     IVAYVIVGGDVLLRAIRNISKGRVFDENFLMALATVGALTIGESSEAVGVMLFYKVGE
                     YLQGVAVGKSRKSITSLMQIRPDYANLKFNSEVKVVSPEEVNVGDIIVVKPGEKVPLD
                     GVVVDGVSMLDTSALTGESVLREVEKGDEILSGVINKNALLSIEVTKSFGESTVSKIL
                     DLVENSSIKKSKTENFISKFSRYYTPIVVIAALLIAFVPPLVISGEVFSDWLYRGLIF
                     LVVSCPCALVLSIPLSFFSGIGFASKNGILIKGSNYLEALRSVDTVVFDKTGTLTKGV
                     FNVTKLNPEGISEEELLEYAAIAEVNSNHPIAKSILSYYNKKIDLDTIDSYEEIAAYG
                     IRVKHNGNFILAGNEKLMKKENISYSSAKEVGTVVYIAVDKVYRGYIVISDEVKEDSK
                     NAIRSLKAIGVKEVVMLTGDNEKVAKNIAQELELDTVYSNLLPNEKVDRLEDLYEGRT
                     EKEKIAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVLMTDEPSKISKAIEIA
                     NKTNKIVWQNIIFALGVKIIVMILGAGGVATMWEAIFADVGVALIAVVNAMRAMR"
BASE COUNT         3609 a          925 c         1535 g         3231 t
ORIGIN      
        1 tataatcaaa taaatatatt tatttgtcat aaattaaatg tccactaaaa tagaaaaaat
       61 aagaagaaat aggtaataaa tgatagctta tagcaaaata aagatgtaag tataacaata
      121 gatgagattg gaattaagtc actataccta acagaacatt cttaggcgaa tatagaagac
      181 ttaaaaaaga aaagagaagt agacaagaat ttattaatga ttacatataa aataaatgaa
      241 ttataatttt attaatgata taataataat taacaaaaaa taaaagagga acgagttaat
      301 gtttgatggc atacactcat tttctaacca ataaaacaat agattcaatt aaggttacta
      361 aagtctaatc aacacataaa catataggat aaatacatct ttttaatata ttaattatgt
      421 tagacttaca aaaaatgtaa atacatagct tgttaaatct tgattgaacc ttaagtaata
      481 agggataaaa aatgacttgt tcaaaggcaa agaatatact attaaatgat ataagtaact
      541 tagagataat atgtagtaag tcaaaagaac tatatagcat cagtaagtaa aaagcgtaaa
      601 aacttacata atgcactata aaataaatga gttagaaaat aaatataatt tatataataa
      661 agtagtacaa aaaggagtat ttatgtttgg atatgttaaa ataaacaaaa tggatttaac
      721 atttagagaa tatgattatt acaaagctta ctattgtgga ctatgtaaat acttaaaaag
      781 aaatcatgga gaaatctcta ggttttcttt gaattatgat ataacatttt taattgtttt
      841 attaacagca gtatataacc cagagtctat aagtacagag gaagtgtgta tagtaaatcc
      901 atttaaaaag aaaaaggtaa ttacaaatga tattacagaa tatgcagcaa gtatgaatat
      961 tcttttaacg tattataaat tggaagataa tttaatggat gataaacgca taaaagataa
     1021 attggcatat tatatatata aaaacaaatt aaaattagca tatgaaaagt atcctgaaaa
     1081 agcagagtat ataaaacaac aactaaatga attaaataag ttagaaaaag ataaaaatat
     1141 aaatatagat gaagtatcta gtatatttgg aaatataatg ggtgaagtat ttgtctataa
     1201 aaaagatgaa aatgagagaa acttaagaat gataggtttt aatataggta agtatatata
     1261 tctactagat gcctatgaag atttagatga agattttaag aaaggaagat ataatccatt
     1321 tatagagtat attgataaaa atgatgagtt aaaagaaaaa gtaaaaaaaa taatagtaac
     1381 atcattagga tttttagcta ggggaattga taatctaaat ctaaataaaa atgttggaat
     1441 aatagaaaat ataatttatt ctggtgttta tttaagatat attaatatat tagaaagtag
     1501 aggtggaaag aatgtacagt aatacttata ataatgaaaa ttttatgaga gcaagagcat
     1561 tcatagaaaa tagtgaattt aaaaaagcat atgatttttt aaaaactttg actgacaaat
     1621 gtgcagagtg gtattattta actggctttt ctgcaatgaa cataggatat tatgaagaag
     1681 gtgaagactt cttaaaaaga gcaaaattta tggagcctga aaatagtgaa tatagtgatg
     1741 cacttagaag ttatacacaa tatagaaatg attatagcaa tagggctgac aattataata
     1801 gaagaagacg taatgattta gatggatgtt gttgctgttg ctgtgatgat tgctgttgtt
     1861 gtctaggtga cgattgttgt gaaaattgtg ctaagctttg gtgtttagat tcatgttgtg
     1921 aatgttttgg tggagattta ataacttgtt gttaggatga taaatatgag tataaaagtt
     1981 gcttatggag gagttttatt agcattaaat gtgatattgt tgactcttac aaatattatt
     2041 cctgtaaata cattgtttat tatggggtta gcatcacttt tagtatcaat tgtaataatg
     2101 gaatggggct ttaagagtgg aatcgctttt tatataggtt ctatagtttt aggatttata
     2161 gtgatggcaa gtaagtctca atggattgta tattcactta cctttggaat atatggtatt
     2221 gtaaaatatc ttatagaaaa agataggtct atatatattg agtattttat gaaattagta
     2281 tttgcaaata taatgatact tatattgtat tttttattaa gaacaatagt ttatattcca
     2341 ataaacatat ttattatagg ttcatttgag atagccttta tagtatatga ctatgtttac
     2401 agttctttta taggttatta caataatagg ttaagaaaga tgctatttaa aaaataatag
     2461 ttgattttta tatttaattt taatgaaatt gtagtttgga taagtgtaaa tgatatatct
     2521 gaatatacat taaagcataa agctaaattt aatatattct tagtgcaaca tataaaagct
     2581 ttgattttta gtcaaagctt ttatatgtta gatataaata ttagatataa tataagtttt
     2641 taaaatagat agtataaatt aaatatccac tatatttaca tctgtagata gagaataaat
     2701 aatatctttt ctttctacta ttatctcaac atttttagaa atttcctcat caagtgatat
     2761 ttgagataaa agttcctttg ttggatttat tgctattgga tttccaactc tttttagcat
     2821 atttatatca ccattagtat ctccataggc atacgagttt gataaatcta aatcatactt
     2881 tacaacaaaa tcatttatag cattattctt acttatagaa tcccacattg gaataactgt
     2941 gcctgtaaat atattatttt caaagacata atcacttcct agaaaatcgg ttacattata
     3001 tttttttgcc attttttcaa caagaaatcc agggctacca gatataaata taactatgtg
     3061 tccttgattt agatgccact caatccttga acgtgtgtat ttataaactc tatctgcttt
     3121 taatttaatt acttggtcac ttgtaaagtt gatacaggtt ttatctaatc cttttagtga
     3181 atcaacataa aggtcacata tttctaatag ataatcatca taattacctt gtcttttatc
     3241 ccaatccata aaagtgtctc tagcatgttt ataccaattt ttttggtcaa ttatatcgta
     3301 tttaattaat tttttaaaat gttcaaccat aagagagtct ctataaagag taccatctat
     3361 atcaaaaaaa gctgctatat ttttcataag accacctcaa ttaatcattt ccttatatta
     3421 tattataaat tgtatctaag ttgttaaaat atatgtataa taatttatat attaaaaaac
     3481 aagtatttta gagtttaaag gatgtgacta tatgaatttt aattttgcaa aggctacaaa
     3541 ttctagactt atgggcagtt taggtcttat aataaattgg atagatgatg aaaacaatca
     3601 cttttgtcaa tattttttat tagatgcaga gggattagga ttagcagatt atgtgagttt
     3661 aaataaccca acacaagaag aagcttatat ggaagaagaa agattgatgg gaggatttgg
     3721 ttcagataga gttgaattaa cgaaggatga atctttattt ttagtatctt attttggtaa
     3781 taagaatctt tattatgata agctattgcc aggagataaa tgtgagtata ttgatattat
     3841 aaaaaactat aaaacagatt taattataga aaaattatac aataaaatat gtaaaagagt
     3901 tgatgaggaa gttgaattta taaactatat gactatgaga tttatagcat gggatagaga
     3961 aagtttaaaa tatttttctg gaagtgacga gattgcaaat atgcatatta ctaatataaa
     4021 tggaacatta cttaaaaacg tggtaagtga taaaggtcaa ggcagatata taagtgaagc
     4081 tttatatgaa gacaatgatg gatattatat atgtaaaatt gctttttgca taagtaagtg
     4141 ctatgaaact ggttttagaa ttaattctct attagtgaca gataaagaac ttatgtatga
     4201 ttttgaggta tttgatgaga tttctaaaag tgaatttgta agtgtttaca gtgtaaattc
     4261 acctgaagaa tttgcaggag tattttatag agaaaatcca tttctgttaa aaagtaatat
     4321 gaatgaaggt atatttttta ctagatttaa ttttaataat gaccatgtaa aagaaaatgt
     4381 atatataata aacaatgata tgaaagctat atattactta atgggaaata agttttttat
     4441 tggtacttat aacgaaaatg attgcaatta tatcaatgaa ataagtcagt ctaattatag
     4501 tgactatatt aaatttgaag agaaattttt ctttgagcaa aatgcattat atgactttgc
     4561 agaatcaggt tcattagatt ttgatgattt tttggaataa taaatttaga cattatttaa
     4621 gcgagattaa ctgaatttaa tctcgcttaa atttatttct ataatattgg gggaatatta
     4681 tgaggaatac atttattatt gtggtcaatt attaaaattt tatacagttt attataaaaa
     4741 atttatactt ttctttatgt tgtttgccaa tattagtctc aaatacgagt accatactat
     4801 tctgacccac caacacaaga aagagttttt taaatgaaaa agtcagataa aattaaattt
     4861 tatctgactt tttctatgtt tatcttatta gattatgtta atatctgttg accgaataag
     4921 cgcatactca gtcaactttc tatacctata ataatattgg gggaatatta tgaggaatac
     4981 atttattatt atggtcaatt attaatgttt tatacaactt gctcaaaaaa atatatatta
     5041 catttcttcc tcatcaaatt cttcaatatt agtagcctgt ggtatacctt gtatagacat
     5101 attactagaa ccataaggag taggatatat attaggaggc atagtactag gcataactac
     5161 aggcatacct tgcattgtat tatgacatat aactcctggt atcatcataa gtacattagg
     5221 aggcatattt tgattacatg agaagttagg aatacccata gtattattca tattataccc
     5281 catattactg ttcatgttat taggcacatt gttaggcata cccatgttac tattcatatt
     5341 attaggcata ttgttaggca tacccatgtt actattcata ttattaggca tattgttagg
     5401 catacccatg ttactattca tgttattagg catattgtta ggcataccca tattactatt
     5461 catattgtta gacatattgt taggcatacc catgttacta ttcatgttat taggcatatt
     5521 gttaggcata cccatattac tattcatatt gttagacata ttattaggca tacccgtatt
     5581 attattcatg ttattaggca tattatttgt agcagatgta ttgtaattca tatttccgga
     5641 tgtattattt gtgttaggca agttgtttgg agaattacta cttttaggat aatgatttac
     5701 attattagga tataggttat ttatgttctg gttattaggt atttttgtat aaccattaga
     5761 aatttgaggg tcatttctca attcatcttg gggaatttct tcattagtaa tataatctgc
     5821 atagttcgct gggtctaagt aaggcatgta actagtatct aaatcctttt ttttcttgtt
     5881 actcatatta aacttgcacc tcctagaaaa ctataaaaaa gtttacaaaa acttattcta
     5941 ataatatata tattaaaaaa gtttataata tgtgcattct aattatatta aattaataaa
     6001 aattattgtt gcaaatttag gaatgatagc atataatgaa catatgaaca gttattcata
     6061 tattaaaaca atggaggatt aaagatgaga gaagagataa atgattgtaa ttgtaatata
     6121 gtacatgaag aaatagtaac agaggctaaa tcaactatgc cagatgaaga gatgttatat
     6181 gatttagcag aattatttaa agtatttggg gatactacta gagtaaagat attatatgca
     6241 ttatttgcaa atgaaatgtg tgtatgtgat atagcgagtt tattaaatat gactcactct
     6301 gcaatatctc atcaacttag agtactaaag caggctagat tagtaaagtt tagaagagaa
     6361 ggaaaaacag tgtattactc attagatgat agtcatataa gccagatatt tgattgtgga
     6421 ttaaatcata ttagagaaac ttataaatga taaatgagag gagtgaatat atatggaagc
     6481 aaataattta attaaaaaag aatttatact aggaggatta aactgtgcac attgtgcaga
     6541 ggagattaat aataaagttt ctaaactaca agaagttaaa tcttccaact taaattttat
     6601 aaataaaaaa cttatagtaa atataaagga aagctttaat gaggatacta ctatagaaaa
     6661 gataatagat ataatagatt caacagaacc aggtcttgat atacaaataa gttctaaaga
     6721 aaatgcatct agtaaaacat caattaaaaa agagcttata ttaggaggat taaactgtgc
     6781 acattgtgca gaggagatta ataacaaggt ttctaaatta aaagaagttg aatcttctaa
     6841 tttaaacttt gtaaataaaa aacttacagt aaatataagt aataacttta aagaggatga
     6901 tgttataaat aaaataaaag aaataataaa ttcaacagag ccaggacttg atatacaagt
     6961 agggtctact gacaaagtaa aagggagaac tactgaaaaa tcaggagctg taaatgatac
     7021 aaataagaaa gaattagttc cacttataat tggagcactt gtatatatat ttggtatata
     7081 tcaaacagct acaggttatg aaagtcagtt tagcaatata gtatttatag ttgcctatgt
     7141 aatagtaggt ggcgatgtgc ttttaagagc aataagaaat atatcaaaag gaagagtatt
     7201 tgatgagaac tttttgatgg ctctagcaac agtaggagca ttaacaatag gggagtcatc
     7261 agaagcagta ggggttatgc ttttctataa agtaggagag tatttacaag gtgttgcagt
     7321 tggtaaatcc agaaagtcta taacttcact tatgcaaata agaccagatt atgcaaatct
     7381 aaaatttaat tctgaagtta aggtagtgtc accagaagaa gtaaatgtag gggatataat
     7441 agtagtaaaa cctggtgaaa aagtaccatt agatggtgta gtagtggatg gagtttctat
     7501 gctagatact tctgcactca caggagagtc agtgctgaga gaagttgaaa aaggagatga
     7561 aatcttatca ggtgttataa ataaaaatgc tttattgagt attgaagtta caaagagttt
     7621 tggagaatca acagtttcta agattcttga ccttgttgaa aattctagca ttaaaaaatc
     7681 aaagacagaa aactttatat ctaagttttc aagatactat actccaatag tagtaatagc
     7741 tgctttatta atagcatttg tgccaccact tgtaatctca ggtgaggtat ttagtgattg
     7801 gttatataga ggattgatat tcttagtcgt ttcttgccct tgtgcattag ttttatcaat
     7861 accacttagt ttctttagtg gaattgggtt tgcatctaaa aatggtatcc ttattaaagg
     7921 aagtaactat ttagaagctt taagaagtgt agatacagta gtatttgata aaacaggaac
     7981 acttactaaa ggtgtgttca atgtaactaa gttaaatcct gaaggtatat cagaagaaga
     8041 attattagaa tatgcagcta ttgcggaggt aaattcaaat catccaatag caaagtctat
     8101 attaagttac tataataaaa aaattgattt agatacaata gacagttatg aggaaatagc
     8161 agcttatgga attagagtaa agcataatgg caattttata ttagcaggta atgagaaact
     8221 tatgaaaaaa gaaaatatat cttattcatc agctaaagaa gtaggcacag tagtctatat
     8281 tgctgttgat aaagtatata gaggttatat agttatatct gatgaagtaa aagaagatag
     8341 taaaaatgcc ataagaagct taaaagcaat aggggtaaaa gaagttgtaa tgttaactgg
     8401 agataatgag aaagtagcaa aaaatattgc tcaagaatta gaattggata cagtttactc
     8461 aaatttactt cctaatgaaa aggtagatag attagaagat ttatatgaag gaagaactga
     8521 aaaagagaaa atagcatttg taggtgatgg aataaatgat gctccagtat tagctcgtgc
     8581 tgatgttggt atagctatgg gaggcttagg ttcagatgct gctattgaag ctgcagatgt
     8641 agtgcttatg acagatgagc ctagcaagat atctaaagca attgaaatag caaacaagac
     8701 taataagata gtgtggcaaa acataatatt tgctctagga gtaaaaatta tagttatgat
     8761 actaggtgct ggaggagtag ctactatgtg ggaagctata tttgctgatg taggtgtggc
     8821 acttatagca gtagtaaatg ctatgagagc tatgagataa gggataaatg taaatccata
     8881 ctatttaaaa aacttataat tatatattga agtatgtaat aatgaaaggt atcaataagt
     8941 ttcttttaac tttattggta cctttttatt ggagcgcttt gcatatataa atttatattc
     9001 attatccatt gtgtttattt aatgtattta tttagtgtag agcttttata ttgtaatttt
     9061 aaaatttaga catagaaggt ttattaataa gtctatatta agtttaaatt agattcataa
     9121 aaattataat acttatgatt gtatatatat tatattaata gttataattt ttacaacaaa
     9181 atgtgtgtgt taaaatactc taaaccaaca gtatgactgt cattcaataa attatgtaat
     9241 ataagaaagt aaatattttt aaaaaataat ttaaaaaagt attgacgaaa tatttttaag
//
LOCUS       DAMJWP010000140         9241 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.145, whole genome shotgun sequence.
ACCESSION   DAMJWP010000140 DAMJWP010000000
VERSION     DAMJWP010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9241
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.145"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            60..689
                     /locus_tag="PC700_002589"
     CDS             60..689
                     /locus_tag="PC700_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581888.1"
                     /translation="MNKYLLINPVAERMYGEKFHLIKANLIKKGYIIAECEPQLEYVK
                     KQYKEYTKKTENTMLDCRCPESISLLKRNNLINNFEVPMIEPILVRTCRVLYDKYIKS
                     KDDMLVVTCPCTQLRDFASEKFKDKGNAMFYTWKEFAEQEGVKSLGKIDESPIPLGYF
                     KDTFEKVLEVSSEDEILSEIQKIRNGFEDKYDIVEMFYCKDGCNNGDGL"
     gene            690..1472
                     /locus_tag="PC700_002590"
     CDS             690..1472
                     /locus_tag="PC700_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2581889.1"
                     /translation="MVNKFNNKFLRLVKSVLIFFIILLLWKITNYLGIWSDYILPSPE
                     KVYSTFLNMISDGSIFINVYASMKRVLIGFAINTAIGVPLGIFFGIYSGVYEYFKSLI
                     NFLRNTPPLALIPMLILWFGIGEESKIIIIVLASFFPIFTSTLKGIKNCDSKLIEVGR
                     VFEFSKLQIIFKIIIPNAILDIAVGLKLALGYSFRAIIGAELVAASSGLGYLISDGKE
                     MSRTDVVIVGIIVIGLLGIITDYIFSIIVKKVSKGKMVEAYE"
     gene            1465..2343
                     /locus_tag="PC700_002591"
     CDS             1465..2343
                     /locus_tag="PC700_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2581890.1"
                     /translation="MNNLVCDSGYIIRNLYKSYKVDNEEHVVLSDISLNIDRNHITVI
                     LGESGCGKTTLLRAIASLEGINSGEIRFINDNKEYRPNVGFVFQESRLMPWLNVSENI
                     AFHNQRKNTILNKVLRKFKHNKRIIGKIINDNFRSLETNDNLNKSNKYNRKSNIDVEK
                     YLKMMNLQKFKNSYPNELSGGMAQRVSIARALSFNPDMLLMDEPFSALDYFTRIDMQN
                     EVIKIHKSTNKGVIFVTHDIDEALKIGKKIVVFTDERKIKEFNIEDGYNRDLTSEYYI
                     KLKKDILMTMKSNKDI"
     gene            2376..3335
                     /locus_tag="PC700_002592"
     CDS             2376..3335
                     /locus_tag="PC700_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2581891.1"
                     /translation="MKLKKLLIGITILTLATASLAGCAKKNNGEKLSEINLTYVKSPL
                     NVPSIIQKQDDLFGKEFKKDNIKVNFHEITTGPEQTQALAAGEIDFLHALGGTSALIA
                     ASNGVELKILNTYSRSPKGFMILTNNNSIKSAADLVGKKVAGPKGTILHQVLISALDK
                     EGLSMDDVEFVNMGIPEASAALSDGSVDVALIAGPAALKAMKSGSKLVANGEGLVDGI
                     IVTAVSTDFAEKHPEIVERFMKVEKETLEYVNNNFDEAMEKVAKEVDLSLEETKELYA
                     WYDFSLDITDKDISSLEDTQDFLIKNKLQEKKVNIKELIYNAK"
     gene            3649..4341
                     /locus_tag="PC700_002593"
     CDS             3649..4341
                     /locus_tag="PC700_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2581892.1"
                     /translation="MSYKVYIVEDDISISTLLKDYIEKYGFEVIVEENFDDIMITFES
                     FKPHLVLLDVNLPKFDGFYWCRCIRQQSNLPIIFISARDGNVDQIRALESGADDYITK
                     PFYYDVVMAKIKSHIRRVYGEYSPKIDERIVELDGLKFYPERLELELNGENLIITKKE
                     GILIECLIKRYPKVVSRDYLLEKIWDDIEFVEENTLNVNVSRIRKRFEELGIDNAVQT
                     VRGLGYKLNKNW"
     gene            4351..5355
                     /locus_tag="PC700_002594"
     CDS             4351..5355
                     /locus_tag="PC700_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2581893.1"
                     /translation="MKLFLKDYKGYIFIYYIGIVMTIIYCNLMKFISFGESLYILLFS
                     SFILLCFLIYKYYKNREVYNLFEKGIHSLEESFLYLGSSFLGRNISVILKQQHKLYQS
                     MIQEHKKIHSEHLTFINQWIHQMKTPISVISLQLQDYEGEEVTENIRKEIDRLNKGLD
                     MAMHFARADNFQKDFIVEKAKLNEIVSSIVNEEKRLFIKSGMIPKIQVDKFTYVYTDV
                     KWMKFIIGQLITNGVKYSKDYSNYLTIKSEENNKYVILSIIDEGIGIVSKDLKRVFDP
                     FFTGENGRKFGESTGMGLYLVKKVCDDLGHKVSIKSEVGKGTEVSIYFNKDVNSLIKM
                     "
     gene            5603..6454
                     /locus_tag="PC700_002595"
     CDS             5603..6454
                     /locus_tag="PC700_002595"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDF2581894.1"
                     /translation="MSLDVIFILKSVIIAIVEGFTEFIPVSSTGHMILVGNLIDFKGQ
                     FAEMFEVVIQLGAILAVVVLYWKKIKDSVIEFFKFIFTGGKEGKIGFRFGMNVIIGCI
                     PFAIIGVLFYDNIKSLFNLQSVIIGFIVGGILLLVVETLFRKKNHSTDNIDKITPIQA
                     LKVGTLQVLSAWPGMSRSASTIMGGWIAGLNSPTAAEFSFFLAVPAMVASSGKDLFEF
                     DYSIMTPTLWIALVVGFIVAFIVSIIVMEKFVNFLKKKPMRVFAVYRIIMGVVLAVLA
                     FTNIISV"
     gene            6558..7304
                     /locus_tag="PC700_002596"
     CDS             6558..7304
                     /locus_tag="PC700_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2581895.1"
                     /translation="MKILTVNNLSKVYGKKIIFNALNDINFSIEDGEFVGIMGPSGSG
                     KTTLLNMISTIDKPTTGTMELKGKNPLLLRGEELALFRRRELGFVFQDFNLLDTLTIG
                     ENIVLPLTLDKVSVKEQDERLNEVSTILGIKDLLGKRTFEVSGGQAQRTAIARALINN
                     PSILLADEPTGNLDSKSSKVVMELFQKINKENKVTTMMVTHDPLAASYCSRILFIKDG
                     SIYNEIYKGSSREQFYQEIMDVLTLLGGDN"
     gene            7304..9220
                     /locus_tag="PC700_002597"
     CDS             7304..9220
                     /locus_tag="PC700_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2581896.1"
                     /translation="MNIKQFAINNISRNIKAYLGYLASIVISSSLLFSFIMFTSHPDL
                     DVSQFPDYLKEGLKMSKIIAYLFLFFFVFYSVSVFLKSRYKEFGILYITGISKRQVMK
                     LIFIENMIINIVSSVIGIIIGLIFAKIFLVVMSTFLELSPLKFYIPFNAILSTLIYFM
                     ILAVLTSIFTYFIVKENQVLKLLKGTKTPKPEPKTSLMLAILSICLFIIAYYNAITST
                     DQYTTYLRLVPVTSLTIVATYLFFSQFSVFFMKKLKLNKRFYRKNTNMLWISNLIYKV
                     KDNARIFFLITITSAVAFTAIGTVYSFWKDVERQINLIYPNTIYYSTMTLHNDPKKPD
                     SKEKDKERMMFIENNLKKENVDYTRTNGEFKTVFPKKSDFNVRIMKESKYLEITETIG
                     VKPISFEDNECISLLSSSLPGNNNVKENIIVGNRGLKVAKQVEECVMPAYYKNMYVVK
                     DNFYDSIESKFIVDRFTAFEVEDSSKTIEICREFENKFNNESGMQPYLFFSKELMIGT
                     GKLIYSTFIFLAIFIGLIFFVTTSSFLYNKFYMDCQVDKKKYEQLNKLGMTYNEIKKA
                     STIEIGIVFLFPYVVAVIHSIFALTALKNSFDIEVASSAVLVMGSLFLVQIVYFLIVR
                     NNYLKEIRLNLVNF"
BASE COUNT         3587 a          872 c         1643 g         3139 t
ORIGIN      
        1 agtgctcatt taaactatta attgataata atattcaata ttaaataata ggaggcgtta
       61 tgaataaata tcttctaata aatccagtgg cagaaagaat gtatggagaa aagtttcacc
      121 ttattaaagc aaatttgata aaaaaggggt acataatagc agaatgtgaa ccacagttag
      181 aatatgtaaa aaagcagtac aaagagtata caaaaaagac tgaaaataca atgttggatt
      241 gtagatgtcc agaaagtata agtcttttaa aaagaaataa tttgataaat aattttgaag
      301 tacctatgat agaaccaata ttagtaagga cttgtagggt tttatacgat aaatatataa
      361 aaagtaaaga tgatatgttg gtagtaacct gtccatgtac acaattaaga gactttgcaa
      421 gtgaaaagtt taaggataag ggtaatgcta tgttttatac atggaaagag tttgctgaac
      481 aagaaggtgt aaaatcactt ggaaaaatag atgaatcacc aattccatta ggatatttca
      541 aagatacttt tgaaaaagtt cttgaagtat caagtgaaga cgaaatatta agtgaaatac
      601 aaaaaataag aaatggcttt gaagataagt atgacatagt agaaatgttt tattgtaaag
      661 atggttgtaa taatggagat ggtttgtaaa tggtcaataa atttaataat aagtttttaa
      721 ggctagtaaa gagtgttctg atatttttta taattctttt attatggaaa attacaaatt
      781 atcttggtat ttggagtgat tatatattac catcacccga aaaggtatat agtacatttt
      841 taaatatgat aagtgatggt tcaatattta ttaatgtata tgcaagtatg aaaagagttt
      901 tgataggttt tgcaataaat actgctatag gtgtgccttt agggatattc tttggaattt
      961 atagtggagt atacgaatac tttaaatctc taataaattt tttaaggaat acacctcccc
     1021 ttgctttaat accaatgctt atattatggt ttggtatagg agaggaatca aaaattataa
     1081 ttatagtttt agcatccttt ttcccaatct ttacaagcac attgaaaggg attaaaaatt
     1141 gtgactcaaa actgattgaa gtgggtagag tatttgagtt ttcaaagctt cagataatat
     1201 ttaaaataat aataccaaat gccattcttg atattgctgt tgggttaaag ttagctcttg
     1261 gatatagctt tagagcaatt attggggcag aacttgtagc agcttcatct ggtcttggat
     1321 acttgatatc tgatggaaaa gaaatgtcta gaacagatgt tgttattgta ggtataatcg
     1381 taattggact tttaggaata attacagatt acatattttc gattattgtg aagaaggtaa
     1441 gtaagggaaa gatggtggaa gcatatgaat aatttagtat gtgactctgg atatataatt
     1501 agaaatttat ataaaagtta taaagttgac aatgaagaac atgtagttct tagtgatatc
     1561 tctttaaaca tagatagaaa ccatataaca gtaattttag gtgaaagtgg ttgtggaaag
     1621 actaccttgc ttagagcaat tgcatcactt gaaggtataa atagtggtga gattaggttt
     1681 attaatgata ataaagagta tagacctaat gttggttttg tttttcagga gagcagatta
     1741 atgccttggt taaacgtaag tgaaaatata gcttttcata atcaaagaaa aaatactatt
     1801 ttaaacaagg tgcttagaaa atttaaacac aataaaagaa ttattggtaa aattataaat
     1861 gataatttta gaagcttaga aacaaatgat aatttgaaca aatcaaataa atataatagg
     1921 aaaagtaata ttgatgtaga aaaatatctt aagatgatga acttgcagaa atttaaaaac
     1981 tcttatccaa atgaattgtc aggaggtatg gcacagagag taagcatagc tagagctctt
     2041 tctttcaatc cagatatgtt gcttatggac gagccttttt cggcattaga ttattttaca
     2101 aggattgata tgcaaaatga agttataaaa atacataagt caactaataa aggcgtaata
     2161 tttgtaactc atgatattga tgaagcgtta aagataggca aaaaaatagt tgttttcaca
     2221 gacgaaagaa aaataaaaga atttaatata gaagatggtt ataatagaga tttaacttca
     2281 gagtactata ttaaattaaa aaaagatatt ttaatgacaa tgaaatcaaa taaagacatt
     2341 taaagaaatt aataatattt taaggagtag agtaaatgaa attaaaaaag ctattgatag
     2401 gtataacgat attaacactt gcaacagcat ctttagctgg atgtgctaaa aaaaataatg
     2461 gagaaaaact ttcagaaata aatttaactt atgtaaaatc accattaaat gttccttcta
     2521 taattcagaa acaggatgat ttatttggaa aggaatttaa aaaggataat ataaaagtta
     2581 actttcacga aattactaca ggtccagagc aaacacaggc tttagctgct ggagaaatag
     2641 acttcttaca tgcactaggt ggtacgtcag ctttaatagc tgcatctaat ggagtagagt
     2701 taaagatatt aaacacttat agcaggtcac caaaaggatt tatgattttg actaataata
     2761 attcaataaa atcagcagca gatttagtag gtaagaaggt ggcaggtcca aaagggacta
     2821 tattacatca ggtattgatt tcagcacttg ataaagaagg attaagtatg gatgatgtgg
     2881 aatttgttaa tatgggtatt ccagaagcat cagcagctct ttctgatggt agcgtagatg
     2941 ttgcattgat tgctggtcca gcagctctta aagctatgaa gtcaggtagt aaacttgtag
     3001 caaatggtga aggtttagta gatggtataa tagtaactgc tgttagcaca gactttgcag
     3061 agaaacaccc agaaatagtt gaaagattta tgaaggttga aaaggaaact ttagaatatg
     3121 taaataataa ttttgatgaa gctatggaaa aagtagctaa agaagttgat ttaagtcttg
     3181 aagaaactaa agaattatat gcttggtatg attttagttt agatataaca gataaagata
     3241 tatcttcgct tgaagataca caagacttct taataaaaaa taagttacaa gagaaaaaag
     3301 tgaatataaa agaattaatc tataatgcta agtagaaact atgctaaaat aatactaagt
     3361 aagaaatata ataaaagttt aagttacctt attaaggtaa ctttttctat tttgaattag
     3421 taatttcaat ttattttaga atgattagtt gataataatg attaattaat cataaaaaga
     3481 tactttttta tagagtttta ttaagatgtc taataatatg gttatactta tgaagtcagt
     3541 aattgtgaag ttaataatat gattaaattt atatattgta aaaagattat aatatattaa
     3601 aattggactg ttatatgatg agtaaatagg tatattagga ggaattatat gagttataaa
     3661 gtatatattg tggaagatga tatatcaata agtacattac ttaaagatta tattgagaaa
     3721 tatggatttg aagtgattgt tgaagagaac tttgatgata taatgattac atttgaaagt
     3781 tttaagccac acttagttct attagatgta aatttaccta aatttgatgg tttctactgg
     3841 tgtagatgta ttagacaaca gtcgaattta cctataattt ttatatcagc tagagatggc
     3901 aatgtagacc aaattagagc tttagaaagt ggagcagatg attatataac aaaacctttt
     3961 tactatgatg tagtaatggc aaaaataaag agtcatataa gaagggtata tggtgaatat
     4021 tcacctaaaa tagacgaaag aatagtggag ctagatggat taaagtttta tccagaaagg
     4081 ttagaactag aattaaatgg tgaaaatcta attataacta aaaaagaagg tatactgatt
     4141 gaatgtctta tcaagagata cccaaaagta gtaagtagag attatctttt agaaaagata
     4201 tgggatgata ttgagtttgt agaagaaaat actttaaatg taaatgttag caggataagg
     4261 aaaagatttg aggaattagg tatagataat gctgttcaga ctgtccgagg gttaggatat
     4321 aaattaaata agaattggta aggagaaagt atgaagttat ttttaaaaga ttataaaggt
     4381 tatattttta tatattatat aggcatagtt atgacaatta tttactgtaa tcttatgaag
     4441 tttattagtt ttggagagag tttatatatt cttttattta gttcttttat attgttatgt
     4501 tttttaatat ataagtatta taaaaataga gaagtatata atctgtttga aaaaggaata
     4561 cattctttgg aggagtcatt tttgtattta ggaagctcat tcttggggag aaatatatca
     4621 gttattttaa aacaacaaca taaattgtat caatcaatga tacaagagca taaaaagata
     4681 catagtgaac atttaacttt catcaaccaa tggattcatc agatgaaaac accaatatca
     4741 gttataagcc ttcagcttca agattatgag ggagaagaag taactgaaaa tataagaaaa
     4801 gaaatagata gattaaataa agggttagat atggctatgc attttgcaag ggcagataat
     4861 tttcagaaag actttatagt tgaaaaagcc aaattaaatg aaattgtatc tagtattgta
     4921 aatgaagaaa aaaggttatt tataaaaagt ggaatgattc ctaagattca agtagacaaa
     4981 tttacttatg tttatacaga tgtaaaatgg atgaaattta taataggtca attaattaca
     5041 aatggagtga aatattctaa agattatagt aactacctaa ctataaaatc tgaagaaaat
     5101 aataaatatg taattttaag tataatagat gaaggaattg gaattgtttc taaagattta
     5161 aaaagggtat ttgacccatt ttttacagga gaaaatggtc gtaagtttgg agaatctaca
     5221 ggtatgggtc tttatctagt aaaaaaggta tgtgatgatt taggtcataa agtaagtata
     5281 aaatcagaag taggaaaagg tacagaagtt tcaatctact ttaataaaga tgtaaatagt
     5341 ttgataaaga tgtaaattat ctgttacaat agggttttta gttaattgta cttttttatt
     5401 atacaattta ttaaatgaaa tattcatagt taaataatca aaatttaaga gagtttattt
     5461 gcaaacctta catagatgta aggtttgtgt aatgtttgca gatagttaaa ttttctaatc
     5521 taaattacaa ttaatataag aaataacatg agagaaataa cataaaaaaa gaaattttgt
     5581 aaaaaaggaa ggatgtttat tgatgagttt agatgttatt tttatattga aatcagtcat
     5641 aatagctata gtagaggggt ttacagaatt tattccagtt tcatctacag gtcatatgat
     5701 tttggttggt aatttaatag attttaaggg gcaatttgct gaaatgtttg aggttgtaat
     5761 acaacttggg gctatattag cagtagtagt actttattgg aaaaagataa aagatagcgt
     5821 tatagaattt tttaaattta tatttactgg cggaaaagaa ggaaagattg gttttagatt
     5881 tggaatgaat gtaattatag gttgtatacc atttgcgatt ataggagttc tattttatga
     5941 taatataaaa tcattgttta atttacaatc tgtaattata ggatttatag tgggtgggat
     6001 tttattatta gtagtagaaa ctttgtttag aaaaaagaat cattcaacag ataatataga
     6061 taaaattact ccaatacaag ctttaaaggt tgggacatta caagttttgt ctgcatggcc
     6121 aggtatgtca aggagtgcat ctactattat gggtggatgg atagcaggac ttaattctcc
     6181 tacagcagct gagttttctt tctttttagc agtaccagct atggtggctt cttcaggaaa
     6241 agatttattt gaatttgatt attcaataat gacaccaaca ttgtggattg ctttggtagt
     6301 tgggtttata gtagctttta ttgtatcaat aatagttatg gaaaaattcg tgaatttttt
     6361 aaagaaaaaa ccaatgagag tgtttgctgt atacagaata atcatgggtg tagtacttgc
     6421 agtattagct tttactaaca taattagtgt atagaaaaaa tagatttatt atctataaaa
     6481 attaaactaa gataaattat aattaacaat tatatatatg ttatttcaaa aattaaatta
     6541 ggaaggatag ttaaataatg aaaattttaa ctgtaaataa tttaagtaaa gtatatggta
     6601 aaaaaataat tttcaacgca ttaaatgaca taaattttag tatagaagat ggagaatttg
     6661 taggaattat gggtccatct ggtagtggaa aaactactct tttaaatatg atttctacta
     6721 ttgacaagcc aactacagga actatggaat taaagggaaa gaatccttta ttgttaagag
     6781 gtgaggaact tgcattattt agaagaagag agttaggttt tgtatttcag gattttaatt
     6841 tattggatac attaactata ggagaaaata tagtattgcc attgacatta gataaggtca
     6901 gtgtaaaaga gcaagatgag agattgaatg aagtatctac aatattaggt ataaaagatt
     6961 tattaggaaa aagaactttt gaagtatcag gtggtcaagc tcagaggact gctattgcta
     7021 gagcattgat taacaatcca tcaatacttt tagcagatga accaacaggt aatttagatt
     7081 caaagtcatc aaaggttgta atggagctat ttcaaaagat aaataaagaa aataaagtta
     7141 ctactatgat ggtaactcat gacccattag cagcgagcta ctgtagtaga atcttattca
     7201 taaaagatgg gtctatatat aatgaaatat ataagggtag tagtagagag cagttttatc
     7261 aagagataat ggatgtactt acattattgg gaggcgataa ctaatgaata taaaacagtt
     7321 tgctattaac aatatttcaa gaaatattaa agcgtattta ggatacttag caagtattgt
     7381 tatatcatct tcactgctat tttcttttat tatgtttaca agtcatccag atttagatgt
     7441 atctcaattt cctgattatt taaaagaagg tctcaagatg agtaagataa ttgcatatct
     7501 attcttattc ttctttgtgt tttattcagt tagtgtgttt cttaaaagta gatataaaga
     7561 atttggaata ctttatatta caggtatatc aaaacgccaa gtaatgaaat taatctttat
     7621 agaaaatatg ataataaata tagtatcttc tgtaattgga ataataatag gcttaatctt
     7681 tgcaaagata tttttagtgg ttatgtctac ttttttagaa ttatcgccat taaaattcta
     7741 tataccattt aatgcgatat tgtctacttt aatctatttt atgattttag ctgtattaac
     7801 atcaatattt acttatttta tagttaaaga aaatcaggtg ttgaagttac ttaaaggtac
     7861 aaaaactcca aaaccagagc ctaaaacatc actaatgctt gctatattaa gtatttgttt
     7921 gtttataata gcgtattata atgctattac ctcaaccgac caatatacaa catatctgcg
     7981 cttagttcca gttacatccc ttactatagt agcaacttac ttgttctttt ctcaatttag
     8041 tgtatttttt atgaagaagc taaaactaaa caagagattt tatagaaaaa atacaaatat
     8101 gctttggatt tcgaacttga tttataaggt aaaggataat gctagaatat ttttcttgat
     8161 aactataact tctgcagtag catttactgc tataggtaca gtttattcct tttggaaaga
     8221 tgtagagcgt caaattaatt taatatatcc taacacaata tattattcaa caatgacatt
     8281 gcataatgac cctaaaaagc ctgatagcaa agaaaaagac aaggaaagaa tgatgtttat
     8341 agaaaataat ctaaaaaaag agaatgtaga ctatacaagg actaatggtg aatttaaaac
     8401 tgtatttccc aagaaaagtg attttaatgt cagaattatg aaagaatcta agtacttaga
     8461 aattacagaa actatagggg ttaagcctat aagttttgaa gataatgaat gcatatcatt
     8521 attatcatca agtttaccag gaaataataa cgtcaaagaa aatattattg tagggaatag
     8581 aggtttaaag gtggcaaaac aagtggaaga atgtgttatg ccagcatatt acaaaaatat
     8641 gtatgtggta aaggataatt tttatgatag tatagaatca aaatttatag tggatagatt
     8701 tactgcattt gaagtagagg attcaagtaa aactatagag atatgcagag aatttgaaaa
     8761 taaatttaat aatgagtcag ggatgcaacc atatttgttt ttctctaaag aattaatgat
     8821 aggaactggg aaattaatat attctacatt tatattttta gcaatattta taggtttaat
     8881 tttctttgtt actacaagta gttttttata taataaattt tatatggatt gccaagttga
     8941 taagaaaaag tatgagcaat taaataaatt ggggatgaca tataatgaga ttaaaaaagc
     9001 ttcaacaatt gagattggaa tagtattttt gtttccttat gtagtggctg ttatacattc
     9061 aatatttgcc cttactgcac tgaaaaattc ttttgatata gaagtagcat catcagctgt
     9121 tttggtaatg ggaagcttgt tcttggttca aatagtttat tttttaattg taagaaataa
     9181 ttatttaaaa gagataaggt taaatttagt caatttttag ttactattat taaatgataa
     9241 t
//
LOCUS       DAMJWP010000141         9187 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.146, whole genome shotgun sequence.
ACCESSION   DAMJWP010000141 DAMJWP010000000
VERSION     DAMJWP010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9187
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.146"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            127..1755
                     /gene="aspD"
                     /locus_tag="PC700_002598"
     CDS             127..1755
                     /gene="aspD"
                     /locus_tag="PC700_002598"
                     /EC_number="4.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 4-decarboxylase"
                     /protein_id="HDF2581897.1"
                     /translation="MKNHTYKEIKNIYGKISPFEFKDKLIDIAKYTAKENNRELLDAG
                     RGNPNWTCSTAREAFFTFGHFAITETRSNWDLGHLAGMPQKKGIKERFFKFINENIDM
                     PGAYLARDIINFGINELGFDGDEFVHELADGIIGDNYPLPDRMLPHMEKIVHDYLVQE
                     MKYDISGKYGDVEIFAVEGATAAMCYIFDSLMANELLKKGDTIALMTPIFTPYLEIPN
                     LPRYDFKVVNINANEVDEKGAHTWQYTKKELEKLRDKSIKALFVVNPNNPASIAMDEI
                     SCNNLIDVIENYNKDLMIISDDVYGTFVEDFSSLMSKLPYNTVGVYSYSKYFGVTGWR
                     LGTFALHKKNVFDKKINDLTGELKKSVDKRYSDMSLNPSSLSFMERVVADSRLVALNH
                     TAGLSTPQQVQMAFFSAFALIDKVDAYKKLNMSICHRRQKLLFEALELPINENKNNAA
                     YYTQFDIEEWAKLNYGEDLFKFISKTASPVDVLYKLANDYSVVLLSGNGFYGPEWSIR
                     ISLANLYDEAYTKIGKAIREILNGYIVEWQKIKK"
     gene            1876..2871
                     /locus_tag="PC700_002599"
     CDS             1876..2871
                     /locus_tag="PC700_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDF2581898.1"
                     /translation="MSIKKKVAIVFTGGTISMTVDDKVGAAIPTLSGEQILSMVTNID
                     KVADVEVFNFDEIPGPHITPYRMMELKNYVNDLLARKDITGVVITHGTDSLEETAYFL
                     DLTIDNIKPVIVTGAMRSSSELGYDGSSNLSASVCTAVSKDAMGKGVLVVLNNEVLLA
                     SEATKTNTLSLNTFKSLTSGPLGIIDCNELVLTRDIVNRTIIDTDKVESKVALFKAYV
                     GENADFIRFAVDSGYKGIVIEAMGIGNIPPQMLSGVEYAREKGLPVVIVSRCHSGRVF
                     DSYGYFGSGRDLKNLGCIFGGDLPGQKARIKLILALGKTDNLDEIKDFFEKGIYC"
     gene            complement(3029..3697)
                     /locus_tag="PC700_002600"
     CDS             complement(3029..3697)
                     /locus_tag="PC700_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDF2581899.1"
                     /translation="METVSNNQSSVEIQLSLFEAIKDLLLINPIFLLGIILIGIFLVV
                     IFKKNSDIPKVKTSILSLAMYYYLCLTLSHIVGIPTFSEYIRLSQLGETFFNPNINLI
                     PFSDGFSLSFILNILLFVPLGFLCPLISKTFERLRNTFLIGFGLSLFIETIQLFTLHR
                     ATDIDDLLTNVIGTIIGYFCFKLIAKLRIVKLHSDDDSMEQDYTVCIPIAIIIVALIL
                     GFFS"
     gene            complement(3796..4108)
                     /locus_tag="PC700_002601"
                     /pseudo
     CDS             complement(3796..4108)
                     /locus_tag="PC700_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431045.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4535..6955)
                     /locus_tag="PC700_002602"
     CDS             complement(4535..6955)
                     /locus_tag="PC700_002602"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDF2581900.1"
                     /translation="MSVYNFKEVESKWQKIWKDNNQYKMDTAQTEKPNYYTLEMFPYP
                     SGKIHMGHVRNYSIGDVVARFKKMEGYNVLHPMGWDSFGLPAENAAIKHGIHPHKWTM
                     ENIEEMKEQLNLLGISYDWDKEVATSTPEYYRFTQEIFLKFLEHGLAYKKKSYVNWCP
                     SCETVLANEQVVQGACERCKATVLKKDLEQWYFKTTEFAEELLNDLDTLDGWPEKVKT
                     MQKNWIGKSTGADLVFDIDGTDKSMTVFTTRPDTTYGVTYMVLAPEHELVKELVAGTE
                     YEADVEAFVQKMHTMTEIERTAADVEKEGMFIGRYVINPLNGKKVPLWIANYVLVEYG
                     TGAIMAVPAHDERDRDFAEKYNLDIIDVITEDNKMINSEEFNGLDASEGFEGIIDKLE
                     KEGRGKRTINYRLRDWLVSRQRYWGCPIPVVYCDECGIVPVKKEDLPVLLPTDVEFTG
                     KGESPLTTSKQFMSTTCPHCGKPARREVDTMDTFVDSSWYFLRYVDSNNENEPFSKEL
                     VNRWHPVDQYIGGVEHAIMHLLYARWFVKAFKSMGMVDFNEPFKNLLTQGMVLMDGSK
                     MSKSKGNTVSPMDIIDEYGADTARLFVLFAAPPERDLDWSEQGVDGCFRFLNRVYRLV
                     DELADVVKKDVEFGELNSQDKDMRYTIHSTLKKVTADLSEKFGFNTAISALMELINDM
                     YKYKELDNINEAVIKEGVQTIVTIIAPFAPHLGEELWTMIGKEGSVFDIDWPKYDEKA
                     LVKDEIEVVVQVNGKVRGKLTVNSNISKDEMEKVALEDEKIKGLVEGKTIVKVVAVPK
                     KLVNIVVK"
     gene            complement(7309..7659)
                     /gene="rsfS"
                     /locus_tag="PC700_002603"
     CDS             complement(7309..7659)
                     /gene="rsfS"
                     /locus_tag="PC700_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDF2581901.1"
                     /translation="MTVEQMTKIAYDAIEDKLGQDTVIINIGKVSSLCDYFIITTASS
                     QRQVKAIADNVEDELAKLGLEPRGKEGQGTQTWVLLDYGDIMVHVFNEENRGFYNLEK
                     LWKDAPYIDIDTLA"
     gene            complement(7776..8348)
                     /gene="yqeK"
                     /locus_tag="PC700_002604"
     CDS             complement(7776..8348)
                     /gene="yqeK"
                     /locus_tag="PC700_002604"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HDF2581902.1"
                     /translation="MNLENINQRLNQMLPVGRLSHSRNVAKCAEKLCEIYGCDKEKAY
                     LAGMIHDCAKYLSDKEIEDYVNKYEIYLDPIEDGNRSLSHSVIGAYICEYEFEVEDED
                     IINAIKYHTTGREDMSLLEKIIYIADLIEEGRKFPVVDTLRELAYGGKLDEALLTSFN
                     NTLMFVINKKEEIHPRTVMARNYLIKEKLL"
     gene            complement(8349..9038)
                     /locus_tag="PC700_002605"
     CDS             complement(8349..9038)
                     /locus_tag="PC700_002605"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HDF2581903.1"
                     /translation="MRSENLVKMAELKNTEKLEKFNRHKGKIKIGILGGTFDPIHYAH
                     LATAEFIRDKYDIDKIIFIPSGNPPHKLCITTDKYDRYNMTLLATESNEDFLVSKVEI
                     ERKKRTYTIDTLKYLKKKYKNADIYFITGADAICSVEEWKDVKKNFELATFIAATRPG
                     ISLLRSQETIEKLTKKYNADIITVYVPSLDISSTYIREQLNEGKSIRYLVPENVENYL
                     YENKLYQYGDD"
BASE COUNT         3022 a         1548 c         1215 g         3402 t
ORIGIN      
        1 agttattatt ttaatttcat actttacttt ttctataaac ctattataat taaagtataa
       61 tcatttttgc aacttgttta ttttattata aaaatgtgaa acttattaca taaggaggaa
      121 tttaacatga aaaatcatac ttataaagaa atcaaaaaca tatatggaaa aataagccct
      181 tttgaattta aggacaaatt aatagatatt gcaaaataca ctgcaaaaga aaataacaga
      241 gaattacttg atgcaggtcg tggaaatcca aactggactt gttcaactgc tagagaagcc
      301 ttttttactt ttggtcattt cgctattact gaaactcgtt ctaattggga tttaggtcat
      361 ttggctggaa tgcctcaaaa aaagggtatt aaagaaagat tttttaaatt tataaatgaa
      421 aatatagata tgcctggagc atatttagct agagatatta taaactttgg tataaatgaa
      481 cttggatttg atggagatga atttgttcat gaactagctg atggaattat tggtgataac
      541 tatccacttc cagatagaat gttacctcat atggaaaaaa tagtacacga ttacctagtt
      601 caagaaatga aatatgatat atcaggtaaa tatggtgatg tagaaatttt tgctgttgaa
      661 ggtgctacag ctgctatgtg ctatatattt gattcattaa tggcaaatga acttctaaaa
      721 aaaggagata caatagcctt aatgactcca atatttacac cttatcttga aatacctaac
      781 cttcctcgtt acgactttaa agtggtaaat ataaatgcta atgaagttga tgaaaaaggt
      841 gctcatacat ggcaatatac aaaaaaagaa ttagagaaac ttcgtgacaa atctattaaa
      901 gctttattcg ttgttaatcc taataatcct gcttctatag ctatggatga aatatcttgt
      961 aataatctaa tagatgtaat tgaaaattat aataaagatt taatgataat atcagatgat
     1021 gtatatggaa cttttgtaga agatttttca tccttaatgt caaaactacc ttacaacact
     1081 gttggagtat attcttattc aaaatatttt ggagttacag gatggagact tggtacattt
     1141 gcccttcata agaaaaatgt ttttgataag aaaataaatg atttaactgg agaattaaaa
     1201 aaatcagtag ataagcgtta cagtgatatg agtcttaacc cttctagtct ttcatttatg
     1261 gaaagagttg ttgctgatag cagacttgtt gctctcaacc atactgctgg tctttctact
     1321 ccacagcaag tacaaatggc gtttttctct gcttttgcat tgattgacaa agtagatgcc
     1381 tacaaaaaac tcaatatgag tatctgccat agacgtcaaa aactattatt tgaagcttta
     1441 gagcttccaa ttaatgaaaa caaaaacaat gctgcatatt acactcaatt tgatattgaa
     1501 gaatgggcta aattaaacta cggagaagac ttatttaaat ttataagtaa aactgcatct
     1561 ccagtagatg ttctttataa attagctaat gactattcag tagttttact tagtggaaat
     1621 ggattctatg gccctgaatg gtcaattaga atatctctag caaatctata tgatgaagct
     1681 tatactaaga taggaaaagc cataagagag atattaaatg gttatatcgt tgaatggcaa
     1741 aaaattaaga aataattata ttacactgaa atatctaaat tatatgaata aaagagttta
     1801 tgtagacctt aactactttt tttgatataa tcctatcgga attataaatt ttatttatga
     1861 aaggcagaga atcgtatgag tattaagaaa aaagttgcca tagtttttac tggtggaact
     1921 atatctatga cagtagatga taaagtcgga gcagctatac caactttatc aggtgaacaa
     1981 atactatcaa tggttaccaa cattgataaa gttgcagatg ttgaagtttt taattttgat
     2041 gaaattccag gacctcacat aacaccttat agaatgatgg aattgaaaaa ttatgtaaat
     2101 gatttattag caagaaaaga tattacaggt gttgtaataa cacatggtac agatagttta
     2161 gaggaaacag cttattttct agaccttaca attgacaaca tcaaacctgt aatagtcaca
     2221 ggtgctatga gaagtagttc tgaacttgga tatgatggtt caagtaattt atctgcatca
     2281 gtttgtactg cagtatccaa agatgctatg ggaaaaggag tccttgtagt tttaaacaat
     2341 gaagtattat tagcttctga agctactaaa acaaatactc tttctctaaa tacatttaaa
     2401 tctctaacta gtggtccatt aggaataatt gactgcaacg aacttgtcct tacaagagat
     2461 attgtaaata gaacaattat agatactgat aaggtagaat ctaaagttgc cttatttaaa
     2521 gcatatgtag gagaaaatgc tgactttata agatttgctg ttgatagtgg ttataaaggt
     2581 atagttattg aggcaatggg tataggtaat attcctcctc aaatgctttc tggagttgaa
     2641 tatgctagag aaaaaggact tcctgtagtt atagtttcta gatgtcattc aggtagagta
     2701 ttcgatagtt atggttactt tggttctggt agagatttaa aaaatcttgg ttgtatattt
     2761 ggtggggact tgccaggtca aaaagctaga ataaaactta tcttagcact tggaaaaact
     2821 gataaccttg atgaaatcaa agatttcttt gaaaaaggaa tctattgcta attctaataa
     2881 cttctattta attatttagt tcagtttaca agaaaataca cacaaaccat aaaatgagat
     2941 gttgacaaca taacagacag tatgctcctg taaaaggtaa catactatct gttatattgt
     3001 ctggattccc aaattagcta tttggaactt aactaaaaaa tcctaatata agggcaacta
     3061 ttataatggc aatcggaata catactgtgt aatcctgttc catagagtca tcatctgaat
     3121 gtagttttac aattcgcaat ttagcaatca gtttgaagca gaaatagcct attattgttc
     3181 caattacatt cgtgagtaag tcgtctatat cggttgctct gtgtaacgta aaaagctgaa
     3241 tagtttcgat aaagagagat aatccaaaac caatcaagaa tgtatttctc aaccgttcaa
     3301 aggttttgct gataagcgga cataaaaaac ctaatggaac aaaaagtaaa atattcaaaa
     3361 taaaactcaa actaaatcca tcactaaaag gaataagatt tatgtttgga ttaaagaacg
     3421 tttcccctaa ctgtgacagt cttatatatt cactgaatgt agggatacca acaatatgcg
     3481 atagtgttaa acataagtag taatacattg ccagtgataa aatagatgtt tttacctttg
     3541 gaatatcact atttttcttg aatattacaa ctaagaaaat accaatcaag ataattccca
     3601 acaagaaaat aggattaatt aacaataaat ctttgatagc ttcaaacaga ctgagttgaa
     3661 tttctacact tgattgatta tttgatacag tctccatttg tacacacccc ctcataacat
     3721 ttcaattatt tctaaaaaac aaaatagaag ataaatctta aaatcatcct atcaaaattt
     3781 ttaaatttgc ctttatcagt ttataccacc agttaattcc tgtcttactt ctgtatggta
     3841 ttcttgggat tctttcatca aattagacaa aaaaatggtt accatatcaa taaactggtt
     3901 acgctccata tctcctgaag caaacatccc ctgagaataa atccgtatca ggaatctcca
     3961 catctactgc acctatcaca agtcatttga taacatcctc aaacaatccc aacttggata
     4021 tatcagcaac acccattaga atactactgc tgaccatcct tgttttcctg gtgataattt
     4081 taatcaactg ggaagttgaa acgcctatct tttttgcaag ttccttttta gaaacatggt
     4141 agttattata tgaatctgaa tctcgttgtc tgggtgttta agataaagca tagatacgca
     4201 ccttttttgc ctgttttcat tatataaaaa aaattttaaa aatacaataa ttatattttt
     4261 ttcgtttaag cttagagcag ttccttgatg aacaacactt tagaaactga ataatttaca
     4321 taccacttaa tacattaaca cgatttaaca ttaattataa taatatttaa aacaacaatt
     4381 agagtgtata agaatgaact ttttagttct ttaatcattt ttttgtataa aatcaaatct
     4441 atttttgtaa tttcaacaat gaaaaaaggc ttatctctat tttgagacag cctcatttat
     4501 tttataaatt tatatttttg ttcatatttt tgagttattt aacaactata tttactaatt
     4561 ttttaggtac agctacaact ttaactatag ttttaccttc aactaatcct tttatttttt
     4621 catcttctaa agctaccttt tccatctcat ctttagatat atttgagtta acagttaatt
     4681 ttcctctaac ttttccatta acttgtacaa caacttctat ttcatctttt actaaagctt
     4741 tttcgtcata tttaggccag tctatatcaa atacacttcc ttctttacct atcatagtcc
     4801 atagttcttc acctaaatga ggggcaaatg gtgcaattat agttactata gtttgtacac
     4861 cttcttttat aactgcttcg tttatattat ctaactcttt atatttatac atatcattta
     4921 ttaattccat aagagctgat atagcagtat taaatccgaa tttttcactt aaatcagcag
     4981 taactttttt aagtgtagag tgtatagtat atctcatatc cttatcttgg gaatttaatt
     5041 ctccaaactc tacatccttt ttaaccacat ctgctaactc gtcaaccaat ctataaaccc
     5101 tatttaagaa tctaaagcat ccatcaacac cttgctctga ccagtctaaa tctctttctg
     5161 gtggtgctgc aaataatacg aaaagtcttg cagtatctgc tccatactca tctattatat
     5221 ccataggaga tactgtattc cctttagact tactcatttt agaaccatcc ataagtacca
     5281 ttccttgagt aagtaagttt ttaaatggct cattgaagtc taccattccc atactcttaa
     5341 atgcctttac aaaccatctt gcataaagca aatgcattat agcatgttct actccaccta
     5401 tatattggtc tactggatgc catctattaa ctaattcctt actaaatggt tcattttcat
     5461 tattgctatc cacatatctt aagaaatacc aagaagaatc aacgaatgta tccatagtat
     5521 caacttccct tctagctggt tttccacagt gaggacaagt tgttgacata aattgctttg
     5581 aagtcgtaag tggagattct ccctttccag taaattcaac atctgttggt aaaagaactg
     5641 gtaaatcttc ctttttaact ggaactattc cacattcatc acaataaacc acaggtatag
     5701 gacaacccca gtatctttgt cttgaaacta accagtctct aagtctataa ttaattgttc
     5761 tctttcctct accttctttt tctaacttat cgattattcc ttcaaatcct tctgaagcat
     5821 ctaatccatt aaattcttct gaatttatca ttttgttatc ttctgttatt acatcaatta
     5881 tgtctaaatt atatttttca gcaaaatctc tatctctttc atcatgtgct ggaactgcca
     5941 ttatagcacc cgttccatac tcaactaata catagttagc tatccataga ggaactttct
     6001 tgccatttaa aggatttata acatatctac ctataaacat tccttctttt tctacatcag
     6061 ctgctgttct ttctatttct gtcatagtat gcattttttg aacaaaagct tcaacatctg
     6121 cttcatattc agtccctgca accaattctt taactaattc atgctctgga gcaagtacca
     6181 tataagtaac tccataagtt gtgtctggtc tagttgtaaa tactgtcata gatttatcag
     6241 ttccatctat atcaaaaact aaatctgctc ctgtactttt acctatccaa tttttttgca
     6301 ttgttttaac tttttctggc catccatcta atgtatctaa gtcattaagt aattcttctg
     6361 caaattcagt agtcttaaaa taccattgtt ctaaatcttt ctttaatact gttgctttac
     6421 atctctcaca tgctccttga acaacttgct catttgcaag aacagtctca caagaaggac
     6481 accagttaac atatgatttt ttcttatatg caagcccatg ctctaaaaac ttcaaaaata
     6541 tttcttgtgt aaatctatag tattctggag ttgaagttgc aacttcttta tcccaatcat
     6601 aactaatacc taataaattt aattgctctt tcatttcttc tatattttcc attgtccatt
     6661 tatgcggatg tattccatgt tttatagctg cattttcagc tggtaatcca aaagaatccc
     6721 atcccattgg atgaagtaca ttatatcctt ccattttttt gaatcttgcg acaacatcac
     6781 ctatagaata atttctaaca tgtcccatgt gtatttttcc agatggataa gggaacattt
     6841 ctaatgtgta ataattaggt ttttcagttt gtgcagtatc cattttatac tgattattat
     6901 ctttccaaat tttttgccat ttcgattcta cttctttaaa attatatacg ctcatatttt
     6961 gcctcctatt tttaattgtg tataactacc ttatttagta ttatttactt ataatttcca
     7021 aataattctt tgaaaataaa aaatcctcgt cactaaactt acgtttaggg acgaggataa
     7081 atcttcgcgg taccacccta attagcctac tacatatata gactctctta gatagctttt
     7141 aatccagcca tggagaatag attttaatct agtaagttca aagttatata atattagttc
     7201 tcaccttcca ctaactctct gaaaaaatat aatcttttac tgctctagtc tattcaattt
     7261 aatataattt ttgaaatttt ctaattataa taacatatat tatcatgttt atgctaatgt
     7321 gtcaatatct atataaggtg catctttcca taatttttct aaattataaa accctctatt
     7381 ttcctcatta aatacatgaa ccattatgtc tccataatca aggagtaccc aagtttgagt
     7441 tccttgtcct tctttacctc ttggctctaa tcctagtttt gctaattcat cttccacatt
     7501 gtcagcaata gcttttacct gtctctgaga agatgctgta gtaataataa aataatcaca
     7561 caaactagaa acttttccta tatttattat aactgtatct tgacccagtt tatcttctat
     7621 ggcatcatat gctatttttg tcatttgttc cactgtcata taaaaattcc tcactttcct
     7681 aattaaaata cacttagtta ttgtaacata ttaatacgat tttgtaacat agtcaaattt
     7741 aaatttatta atatgtttgc ctttatatag cttttttata ataacttttc tttaattaga
     7801 tagtttcttg ccataactgt tcttggatgt atctcttctt ttttattgat gacaaacatt
     7861 aaagtattat taaaagaagt taaaagtgct tcatcaagct tccctccata tgctaattct
     7921 cttaatgtat ctacaactgg aaattttctt ccttcttcaa ttaaatcagc tatgtaaatt
     7981 attttttcta aaagagacat gtcctctctc cctgttgtat gatatttgat tgcatttata
     8041 atatcttcat cttctacttc aaattcatac tcgcatatgt acgccccaat tacactatga
     8101 gacaaggaac gatttccatc ctctattggg tctaaatata tttcatactt atttacataa
     8161 tcctctattt ccttgtcact taaatacttt gcacaatcat gaatcattcc tgctaagtaa
     8221 gctttttctt tgtcacatcc ataaatctca cataattttt cagcacattt tgcaacattt
     8281 cttgaatggc taagcctccc cactggtagc atttgattta gtctttggtt aatattttct
     8341 aagttcattt aatcatctcc atattgatat agtttatttt catataaata attttctaca
     8401 ttttcgggta ctaagtaacg tattgacttg ccttcattta gctgttccct tatatatgtg
     8461 gacgatatat ccagtgatgg aacataaact gttataatgt cagcattata cttcttagtt
     8521 agtttttcta tagtctcttg tgaccttaac aaactaattc ctggtctagt tgcggctata
     8581 aaagtagcta actcaaagtt tttctttaca tctttccact cttcaacact acaaattgca
     8641 tctgcacctg ttataaaata aatgtctgca tttttgtatt ttttctttaa gtattttaat
     8701 gtatctattg tataagtcct tttctttctt tcgatttcta cttttgaaac taaaaaatct
     8761 tcgttgcttt cagttgctag aagtgtcata ttatatctat catacttatc tgtagtgata
     8821 cataatttat gtggtggatt tcctgatggt ataaagatta ttttgtcaat gtcatacttg
     8881 tctctaataa attctgctgt tgctaaatga gcatagtgta tagggtcaaa agtacctccc
     8941 aatataccta ttttgatttt gcctttatgg cgattaaatt tctctagttt ttcagtattt
     9001 ttcaactctg ccatcttaac tagattttca cttctcatat tttgtctcct tttaatgtta
     9061 taatactatt gtaccattat tttttatttc ttaaagctta taatttgttt ttttgggtat
     9121 ataattatat ttatatgcta aaaaacactt taaaaaataa ttatttaaca tgattctgct
     9181 ttacttt
//
LOCUS       DAMJWP010000142         9177 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.147, whole genome shotgun sequence.
ACCESSION   DAMJWP010000142 DAMJWP010000000
VERSION     DAMJWP010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9177
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.147"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(73..264)
                     /locus_tag="PC700_002606"
     CDS             complement(73..264)
                     /locus_tag="PC700_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HDF2581904.1"
                     /translation="MIRVCPNCSNVDVNKLKTLVGEENVKTGCIGQCRAFKKEAVGII
                     DNELEIKETEAEFFEACKE"
     gene            complement(412..654)
                     /locus_tag="PC700_002607"
     CDS             complement(412..654)
                     /locus_tag="PC700_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581905.1"
                     /translation="MLEVTKIIIKYFASESDTFIKEEVCNFEELLKWSHKFIEGKKIE
                     DVLYIKSKENYKVNVIKSLEIKELEIVRKQQIPPYM"
     gene            866..2689
                     /gene="typA"
                     /locus_tag="PC700_002608"
     CDS             866..2689
                     /gene="typA"
                     /locus_tag="PC700_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HDF2581906.1"
                     /translation="MSQKHKIINIAVIAHVDAGKSTLVDAFLSQSGVFRKNEVVKDCV
                     MDSNDLEKERGITIYSKNCAINYEDYKINIVDTPGHSDFSSEVERVMKTVDTVILLVD
                     ASEGPMPQTRFVLQKSLEFGLKPILFINKIDKKDQRAEEVVNEVFDLFVDLNATDEQC
                     EFPIIYGIAKQGIAKLEMDDDSEDLSPLFKTIVNHVEAYPNYDNEPLQFQISALAYDD
                     YVGRLGIGRIYKGTLKNNTQVAICREDSVVSKGKVSKLSVYEGLKQVEVDEATSGEIV
                     VIAGIPDISIGETICDLDSPLPMEMIKIEEPTLSMNFLVNDSPFVGKSGKFVTTRHLK
                     DRLEKELEVNVGLKVEPLDTTDGYKVSGRGELHLSILLENMRREGYEVGVSKPEVLMH
                     KEDGKLMEPIERVVVNCPEVYSGTIINELNMRKGMMESMSIEGDYVKIEFLAPTRGLL
                     GYRSEFINATRGEGTLVRSFEKFEEFKGEIPSRGNGVLIAQGPGVTMGYSLNALSDRA
                     VMFVDPGVEVYEGMIIGMNSRKDDMVVNPCKNKKMSNVRASGSDDAIKLSPPRIFTLE
                     EALEFIEDDELVEITPDSIRLRKRFLNEHDRLRYNKSRQGK"
     gene            complement(2779..3687)
                     /gene="trxB"
                     /locus_tag="PC700_002609"
     CDS             complement(2779..3687)
                     /gene="trxB"
                     /locus_tag="PC700_002609"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDF2581907.1"
                     /translation="MVDIIVIGAGPAGLTSAIYAMRAGLSVTVFEKNIYGGQVASTSE
                     VENYPAVQKISGVEFSNNIYNQAVAQGVDIQFDEVEEINLEGKVKVVKTSSGEHKAKA
                     VILANGVERRKLGCAGEQEFTGRGVSYCATCDGAFFKDKEVAIVGGGNTALEDALFLA
                     NNCTKVYLIHRRDSFRGEEVLEKSVKARENIEILYSHGVEKIEGEKTVSKIEVKNLKT
                     EEKRTIDVSGIFIAIGLKPNNKMFENVLDLDEGGYIISDESCTTSVEGVYVAGDSRTK
                     FLRQIITAASDGAIAAVQAANYINVE"
     misc_binding    4489..4720
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4828..6309
                     /locus_tag="PC700_002610"
     CDS             4828..6309
                     /locus_tag="PC700_002610"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HDF2581908.1"
                     /translation="MYYSSTRGTEEKVTASQAIIKGISSDGGLYVPSEFPNVKNELIN
                     LVNLTYSQIAFFVLSKFLCDFTEDEIKNCIENAYDEKFDCSSIAPLNKVNDTYFLELY
                     HGPTLAFKDMALTIMPHLLKTSIKKDNLEKDVVILTATSGDTGKAALEGFKDIDKIKI
                     IVFFPEDGVSPVQKLQMKTQTGKNTYVVGIKGNFDDAQSGVKKIFSDKKFNQKLLDNN
                     YILSSANSINIGRLLPQVAYYFYSYMKLVSNSEINLNDKINFVVPTGNFGNILAGYYA
                     KQMGLPINKLICASNDNNVLYDFFKTGVYDKNRTLKLTTSPSMDILISSNLERLLFEI
                     SNRNTDIVNKLLSDLSDKGVYKISDDMEKNLASFYGEYSNEEEVKSTINNVFKNYNYL
                     IDTHTAVAYNCYEKYKKETSDNTKTVIVSTASPFKFSEDVLKSIDCDFKDLDDFAIID
                     RLSSISKIDIPKPIKNLKNAEILHKDIYEKDELKLAIKKFLKV"
     gene            6319..7212
                     /locus_tag="PC700_002611"
     CDS             6319..7212
                     /locus_tag="PC700_002611"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HDF2581909.1"
                     /translation="MLEIIVPATSANIGPGFDCLGIALNIYNKFYVEEIESGLEIEGC
                     EDAYKNENNLVYTSMKYFFDRVKPEKIPAGIKIKIHSEVPICRGLGSSASCIVAGVIA
                     ANALSGANLDKNQLLNIASEIEGHPDNVAPAILGNMIVSVTDNENIHYDIIKIPEELK
                     FCAMIPNFKLSTEKARGVLPKEIPYSDGVFNVSRVALLISALLNKNFDLLKVACQDKL
                     HQNYRGTLIENYNDIVEKSEQLNSIGVFLSGAGPTIMSLIKENDDSFVDNMKNYLQKL
                     KSDWEIKELCCDSNGAVLNII"
     gene            7336..8397
                     /locus_tag="PC700_002612"
     CDS             7336..8397
                     /locus_tag="PC700_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HDF2581910.1"
                     /translation="MIIKELIIRDLVAKVPIIQGGMGVGISRSSLAGAVAKLGGVGVI
                     SGVQIGYDEEDFETNTINANLRAIKKHISKAKEISNGGIIGINFMVAMKEYETYVKEA
                     VKAGVDLIISGAGLPNKLPSLVKGSNVKIAPIVSTAKAANVILKMWDRKEKTTADLIV
                     VEGPKAGGHLGYSNEELDNIDSIDYDKEFVEILKVANTYGEKFGRNIPVVAAGGITSS
                     SDVKKYIDMGASGVQVGTRFVATYECDAHENFKMAYINASEEDVHIVKSPVGLPGRAI
                     RNKFIEEVKINRPEIKKCYNCLIPCNPKETPYCISQALINAVKGDVENSLLFCGNDAY
                     KIDKLSTVEDVINELISEL"
     gene            complement(8552..9064)
                     /locus_tag="PC700_002613"
     CDS             complement(8552..9064)
                     /locus_tag="PC700_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDF2581911.1"
                     /translation="MSDFKEILAEEIKNNPFGLIGKDWTLITAENEGKVNTMTASWGG
                     LGVMWGKDVAFVVIRPQRYTKEFIDNTDKFSLTFFDEDFRKELSYCGKVSGREEDKIS
                     QIGFNIEHLNDTPYFKEAKMAIICKKIYSQKLEPQCFIAEGIDGRWYPQKDYHTLYIA
                     EITNVLVKED"
BASE COUNT         3292 a         1286 c         1316 g         3283 t
ORIGIN      
        1 gagcatctaa attgtttaat gcttatacaa tttagatgct ctatttatta taatttatat
       61 tttatttaaa atttattctt tacatgcttc aaaaaactct gcttctgttt cctttatttc
      121 aagctcattg tctattattc caacagcttc ttttttaaat gctctacact gacctataca
      181 accagtcttt acattttctt ctccaaccaa agtttttagc ttatttacat ccacatttga
      241 acagtttgga catactctta tcatatttaa gttcctccta attaatgtct aattaatact
      301 tttataccca aatataaact ttataaaagt ttttttaata taaattatta ccatatatac
      361 catttatatc ctattgtgag tattacatat taattttata tattattaat actacatgta
      421 cggtggtatc tgttgttttc tcactatttc caattcctta atttctaaag acttaatcac
      481 atttacttta tagttttctt tagactttat atacaaaaca tcttctatct ttttaccttc
      541 aataaattta tgagaccact taagaagttc ttcaaaatta caaacttctt cttttataaa
      601 agtatcactt tcacttgcaa aatatttaat tattattttg gttacttcca acatatatca
      661 tcctttactc ttttattaat ctttttatct attaagatta ttatttgtct aaaaaatttt
      721 tttaaacaga ataattttca gatgcaaaac taacagtttt atccaattta aactatatta
      781 atacaattta atatattcct ccttgtacct aataatatat tgtgctataa ttaataagtt
      841 attttaatcg aagggagcaa caaaaatgtc acaaaaacat aaaattataa atatcgcagt
      901 tatagcccac gttgacgctg gaaaatcaac attggttgat gctttccttt ctcaaagcgg
      961 tgtttttaga aagaacgaag tagttaaaga ctgcgttatg gacagtaacg accttgaaaa
     1021 agaaagaggc ataactatat attcaaaaaa ctgtgcaata aactatgaag attacaaaat
     1081 aaacatagtt gatactccag gacacagtga cttttcttct gaagttgaac gtgttatgaa
     1141 aactgttgac acagttattc tattagttga tgctagtgaa ggcccaatgc ctcaaactag
     1201 attcgtttta caaaaatctt tagagttcgg tttaaaacca atattattta taaacaagat
     1261 tgataaaaaa gaccagagag cggaagaagt agttaatgaa gttttcgatt tatttgtaga
     1321 cttaaatgca acagatgagc aatgcgaatt tcctataata tatggtattg ctaaacaagg
     1381 tattgctaaa cttgaaatgg atgatgatag tgaagacctt tctcctctat ttaaaactat
     1441 tgtaaatcac gttgaagctt atcctaatta cgataatgaa cctctacaat tccaaatatc
     1501 agctcttgca tatgatgatt atgtaggtag acttggtata ggtagaattt acaaaggtac
     1561 tcttaaaaac aatacacaag ttgctatatg tagagaagat tctgttgtat ctaaaggaaa
     1621 agtatcaaaa ctttctgtgt atgaaggttt aaaacaagtt gaagttgatg aagctactag
     1681 tggagaaata gttgttatag ctggtattcc tgatatatct ataggtgaaa ctatatgcga
     1741 tttagatagt ccactaccta tggaaatgat aaaaatagaa gagcctactc tttctatgaa
     1801 cttcttagta aacgactctc cattcgttgg aaaaagtgga aaattcgtta caactagaca
     1861 tttgaaagat agacttgaaa aggaacttga agtaaatgtt ggtcttaaag ttgaacctct
     1921 tgatactact gatggataca aagtttctgg acgtggagag cttcacttat ctatactact
     1981 tgaaaatatg agacgtgaag gttatgaagt tggtgtttct aaacctgaag ttttaatgca
     2041 taaagaagat ggaaaactaa tggaaccaat agaaagagtt gttgtaaact gtcctgaagt
     2101 ttactctggt actattataa atgaattaaa catgagaaaa ggtatgatgg agtctatgtc
     2161 aatagaagga gactatgtaa aaatagagtt cttagcacct actcgtggtc ttttaggata
     2221 tcgaagtgaa tttataaacg ctactcgtgg agaaggtact ttagttcgtt catttgaaaa
     2281 atttgaagaa tttaaaggag aaataccttc aagaggaaat ggtgttttaa tagcacaagg
     2341 acctggtgtt actatggggt attctcttaa tgccttaagt gatagagctg taatgtttgt
     2401 agaccctggt gtagaagttt acgaaggtat gataattggt atgaattcca gaaaagatga
     2461 tatggtagtc aacccttgta aaaacaagaa aatgagtaac gtacgtgctt ctggttcaga
     2521 tgatgctata aaattatctc ctcctagaat atttactctt gaagaagcac tagagtttat
     2581 agaagatgat gaattagttg aaattacacc tgattctata agacttagaa aaagattctt
     2641 aaatgaacat gatagattaa gatataacaa atctagacaa ggtaaataaa acgtacattt
     2701 acctctaatt aacaaaatag ccatatagta ctgaaaaatt atttcagttt atatggctat
     2761 ttttatttga aatttatatt attcaacatt tatataatta gctgcttgaa cggctgcaat
     2821 agctccatct gatgctgctg ttattatttg acgtaaaaac ttagttctac tatctcctgc
     2881 tacatataca ccttctactg atgttgtaca agactcatct gaaattatat atccaccttc
     2941 atccaaatca agaacatttt caaacatttt attattaggt ttaagaccta tagcaataaa
     3001 aataccacta acatctatag ttcttttttc ttcagtcttt aagtttttta cttctatttt
     3061 cgatacagtc ttttctcctt ctattttttc tacaccatga ctgtaaagta tctcaatatt
     3121 ctctcttgct ttcacagatt tctccaacac ttcttcacct ctaaaactat ctcttctgtg
     3181 aattaaatac acttttgtac aattattagc taaaaataaa gcatcttcta gtgcagtatt
     3241 tcctccacct actatagcaa cttctttatc tttaaaaaag gctccatcac atgttgcaca
     3301 gtaagatact cctctacctg taaattcttg ttcccctgca caacctaatt tacgtctttc
     3361 aactccattt gcaagaataa cagcctttgc tttatgttca cctgaagaag tttttacaac
     3421 ctttactttt ccctctaaat ttatctcttc aacctcatca aactgaatat caacaccttg
     3481 agcaactgct tgattgtata tattattaga aaattctacc ccagaaatct tttgaactgc
     3541 tggataattt tctacctcag aggtacttgc aacttgacct ccataaatat ttttttcaaa
     3601 tactgtaacg cttaaccctg ctctcatagc ataaattgct gaagttaaac ctgctggtcc
     3661 agcaccaatt acaatgatat ctaccataat gtttacctct ctttatatta ttatttctta
     3721 tatattattc tcatcattct tatactttac tattattatc catatatact acaaacaaaa
     3781 cattattttt tattaattaa ctttatttta catatatata aaaaaactgc aaataaaaat
     3841 taacttttat ttacagttct cacttattta tataattcaa aaccttattg gttatcattt
     3901 acagtgtctt ctaatatatt gctttctgat atatatgata gaattgaaag tccatacata
     3961 accaaaacta atgctgctgt tataagtact attcccatat ttaatacctc cttttgttta
     4021 cagtatacta ttattatgtg aaatatttat gaatttaatt tgaatatata tgtaatttca
     4081 cactaacaat ttcttcaaat taaacattat taactatata cttttgttga accaattatg
     4141 attaatagtt taaatattag cactatttac ttatatttat atgatatacc atataattac
     4201 atttttcaaa ttaaagtttt gtaaactttt tttgtaatat agtgtagtat aaaattgtaa
     4261 tattacagat atttaatagt tatcttttga ttactttcat taaaaacagc ttcatcacta
     4321 tgaaaatcta ggtttttaaa cttttaaaaa agttaaaaaa atatttttct aatactcaat
     4381 ttgattacaa tttttttaca atttaaaaaa tattttttcc aaatttttat attaatactt
     4441 gactttgtct gttttataaa ctagaataat taataatatt aagaatattg aaaggcattg
     4501 attaggaaga gtaaatctga atcgattacc agagaaggaa actactagct gaaatgtttc
     4561 cctaattaga aggattgaaa actacctagg agcttctatc cgaagttatc ttaatgattt
     4621 tagtaggaat agacgttttt attacttgcg ttaaagtatc aagtggattt tttaaaatca
     4681 atttgggtgg aaccgcggga aacagattct cgtccctttt ttagggatga gggtcttttt
     4741 ttattaccta aattcggttt ttaaaatatt ctgtatctaa tttaattagc taattaaaaa
     4801 tttaaaatat atggaggatt ataaaaaatg tattattcaa gtacgagagg caccgaagaa
     4861 aaagtaactg catcacaagc aataataaaa ggaatatcaa gtgatggtgg tctttatgtt
     4921 ccaagtgaat ttcccaatgt aaaaaatgaa cttataaatt tagtaaattt aacgtattct
     4981 cagatagcat tttttgtact tagtaaattt ttatgtgatt ttactgaaga tgaaattaaa
     5041 aattgtatag aaaatgcata tgatgaaaag tttgattgta gtagtatagc tccattaaat
     5101 aaagtaaatg atacttattt cttagaacta tatcacggtc caactttagc atttaaagat
     5161 atggcactta ctataatgcc tcatcttctt aaaacatcta taaagaaaga taatttagag
     5221 aaagatgttg tgattcttac tgctacatct ggagatacag gcaaagctgc acttgaggga
     5281 tttaaagata tagataaaat aaagataatt gtattcttcc cagaagatgg tgtaagccca
     5341 gtacaaaaac ttcaaatgaa aactcaaact ggaaaaaata catatgtagt tggtataaaa
     5401 ggaaattttg atgatgcaca atcaggtgta aagaagattt tttctgataa aaaatttaat
     5461 caaaaactat tggataataa ttatatatta tcttctgcaa attcaattaa tataggaaga
     5521 cttcttccac aagttgcata ctatttttat tcatatatga aattagttag caatagtgag
     5581 ataaatttaa atgataaaat aaattttgtt gtcccaactg gtaattttgg taatattcta
     5641 gctggatatt atgccaaaca aatgggtctt ccaataaaca aattgatatg tgcatcaaat
     5701 gataataatg ttctttatga tttctttaaa actggtgtat atgataaaaa tagaacattg
     5761 aaattaacta cttctccttc tatggatata ttaatttcaa gtaatttaga acgattactg
     5821 tttgaaatat caaatagaaa tactgatatt gtaaataagc tgttatcaga ccttagtgat
     5881 aaaggagtct acaaaataag tgatgatatg gaaaaaaatt tagcctcatt ctatggagaa
     5941 tattcaaatg aagaagaagt taaaagcacg ataaacaatg tgtttaaaaa ttataattat
     6001 ttgatagata ctcatactgc tgttgcatac aattgttatg aaaaatataa aaaagaaact
     6061 tctgataaca caaaaactgt tattgtaagt actgcaagtc cattcaagtt ttctgaagat
     6121 gttctaaagt ctatagattg tgattttaaa gaccttgatg actttgctat aattgataga
     6181 ttatcaagta tttctaaaat agatattcca aaacctatta aaaatcttaa aaatgcagaa
     6241 atactacata aagatattta tgaaaaagat gaattaaaac tagctataaa aaaattctta
     6301 aaggtatagg tgattattat gttagagatt atagttcctg caacaagtgc taatataggt
     6361 ccaggatttg attgtcttgg aattgcgtta aatatataca ataaatttta tgttgaagaa
     6421 attgaaagtg gtcttgaaat agaaggttgt gaagatgctt acaaaaacga aaataactta
     6481 gtttatacat ctatgaagta cttttttgat agagttaaac cagaaaaaat acctgctggt
     6541 atcaaaataa aaatccacag tgaagtacct atatgtagag gtcttggcag tagtgcctct
     6601 tgtatagtag ctggagttat agctgcaaat gctctatcag gtgctaattt agataaaaat
     6661 caattattaa atatagcttc agaaatagaa ggtcacccag ataatgttgc tcctgctatt
     6721 ttaggaaata tgatcgtatc tgttactgat aatgagaata tacattatga tattataaaa
     6781 attcctgaag aacttaaatt ttgtgcaatg atacctaatt ttaagttatc aacagaaaaa
     6841 gctagaggtg ttttaccaaa ggaaatacca tattctgatg gggtattcaa tgttagtaga
     6901 gttgctttac taatatctgc cttactaaac aaaaactttg acctacttaa agtagcttgt
     6961 caagacaaat tgcatcaaaa ttatagagga actctaattg agaactacaa tgatattgtt
     7021 gaaaaatctg aacaattaaa tagtattgga gtatttttaa gtggagctgg acctactata
     7081 atgtctttaa taaaagaaaa tgatgatagt tttgtggata atatgaaaaa ttacctacaa
     7141 aaactaaaat ctgattggga aataaaagag ttatgttgcg attctaatgg agctgtttta
     7201 aatataatat aattatcgtc ttctatacaa aatataacta ctcttttatt ataatttgta
     7261 gtataataat taggactagg taataatatt aagtattata atctagttct ttaaacatgt
     7321 ttacattgag gagggattat cataaaagag ttaataatta gagatttagt tgcaaaagtt
     7381 cctataattc aaggtggaat gggcgttggt atatctaggt catcactagc tggtgcagtt
     7441 gctaagcttg gtggtgttgg tgtaatttct ggagttcaaa ttggatatga tgaggaagat
     7501 tttgaaacta atactataaa tgcaaattta agagctataa aaaaacatat ttctaaggcc
     7561 aaagaaatct caaatggtgg tattattggt attaatttta tggtagctat gaaagaatat
     7621 gaaacatacg taaaggaagc tgttaaagct ggtgtagacc ttataatatc tggagctgga
     7681 ttacctaata agctaccatc attagtaaag ggaagtaatg taaaaatagc acccatagtt
     7741 tcaactgcaa aagctgctaa tgtaatttta aaaatgtggg atagaaaaga gaaaactact
     7801 gctgacttaa tagttgttga aggtccaaaa gctggtggtc accttggata ctctaacgag
     7861 gaacttgata acatagattc tatagactat gataaggaat ttgttgaaat tttaaaggtt
     7921 gcaaatactt atggagaaaa atttggtaga aacataccag tagttgctgc tggtggtata
     7981 acttctagta gtgatgttaa aaaatatata gatatgggtg caagtggtgt acaagttggt
     8041 acaagatttg ttgctactta cgaatgtgat gcccatgaga actttaaaat ggcatacata
     8101 aatgctagtg aagaagatgt tcatatagtt aaaagtcctg taggtcttcc tggacgcgcc
     8161 ataagaaaca aatttataga agaagttaaa ataaatcgtc cagaaataaa gaagtgctat
     8221 aattgtttga ttccttgtaa tccaaaagaa actccttact gtatttctca agctctaatc
     8281 aatgcagtta aaggagatgt tgaaaattca cttttatttt gtggaaatga tgcctataaa
     8341 attgataaat taagtactgt tgaagatgtt atcaatgaat tgatttctga attataattt
     8401 aaagaaaact aaattaaaat tttatcaagc taaggagttt taatacgtta tttattaatc
     8461 tttataaaca tatttatgtc cttagctttt ttgtgtcaat ttccttttat tgaaatttta
     8521 atttaattag agatagtatt aacccttata actaatcttc ttttacaagt acattggtta
     8581 tttcagctat atataaagta tgataatctt tttgtggata ccatcttcca tctatacctt
     8641 cagcaataaa acattggggt tcaagtttct gactataaat ttttttgcat ataatagcca
     8701 ttttagcttc cttaaaataa ggtgtatcat ttaagtgttc tatattaaat ccaatctgtg
     8761 atatcttatc ctcttctcta ccagatacct ttccacaata tgaaagctct ttacgaaaat
     8821 cttcatcaaa aaatgtaaga gaaaatttat ctgtattatc tataaattcc ttagtataac
     8881 gctgtggtct tattactaca aaagcaacat cttttcccca cataactcca agtcctcccc
     8941 agctagcagt cattgtattc actttacctt cattttctgc tgttataagt gtccaatctt
     9001 tacctatcaa tccaaatgga ttatttttaa tctcttctgc caaaatttct ttaaaatcac
     9061 tcatatctct aataacctct cattctctta tatttatatt ccttttattt tcgtgataaa
     9121 ttataaatga agtctagttt tgaaaataaa atactttttt aaatatatta ttgcaaa
//
LOCUS       DAMJWP010000143         9124 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.148, whole genome shotgun sequence.
ACCESSION   DAMJWP010000143 DAMJWP010000000
VERSION     DAMJWP010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9124
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.148"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            154..474
                     /locus_tag="PC700_002614"
     CDS             154..474
                     /locus_tag="PC700_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HDF2581912.1"
                     /translation="MKITLPETAIDTLKSILKDNQDKPNNIRVYFAGVGCGGPSFGLA
                     LDEKKEDDLTYEVGELQFVMSSDEYSQYGDIIIEDTGFGFRVIPENMKDQGGGGCSGC
                     SGCH"
     gene            612..1256
                     /gene="trmB"
                     /locus_tag="PC700_002615"
     CDS             612..1256
                     /gene="trmB"
                     /locus_tag="PC700_002615"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDF2581913.1"
                     /translation="MRRRRKKGADEKLLSYTKYVLRDDIDKLKGKWNLKFRNDNPIHV
                     EFGTGKGKFITTLAKQNPDINYIAMELKEEVLLKAVEKADASNLNNILFLWGDVSNIL
                     DYFEAKELSRIYINFCDPWPKNRWSKRRLTHSGFLEMYNRVLEDDGEIHFKTDNEKLF
                     EFSLNEIAANNWLLKNISLDLGNSEYENNVTTEYEDKFMSQGMRIFRCEAKKRN"
     gene            1385..2131
                     /locus_tag="PC700_002616"
     CDS             1385..2131
                     /locus_tag="PC700_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581914.1"
                     /translation="MWEGTMNLANLIPFIISAAALSPINSNDKVLSENQLKDIFPDAN
                     LRAVVKRYINPDEMTISNIKALDGEFYATGESISNLKGISYLENVDNFIFWNNNIKEV
                     PKEALSLKDMDSINLANNYLIDDDVVNSLSHNGVDVNCDLNFIDTKDNQYKLDSKYHN
                     VDIAKGESIDLRKIITKKIDSYYKYWEVTDNLPKDLDFIVSVSDKSVLSCEDMIIKGN
                     KTGQAVVKVILSDKNDLNTSQEVLITVNVK"
     gene            complement(2260..2538)
                     /locus_tag="PC700_002617"
     CDS             complement(2260..2538)
                     /locus_tag="PC700_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581915.1"
                     /translation="MVDTLEFGLKILFFILSIIWMGKIMILRTDKQIVINPLLIGISA
                     VLVMLHTSQSNIEFFGLDVQYIRIVLYIIYSLIILIGIWATNRRNGIF"
     gene            2813..3259
                     /locus_tag="PC700_002618"
     CDS             2813..3259
                     /locus_tag="PC700_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581916.1"
                     /translation="MESKFFNWYTQSLGGTFGIISCICAYLNGYMFVYTNSASKYFES
                     IGFLGIISSYTLLPLCMLTLIFGVIYSYTPETSDKSIFNIPFFTLNKRLILITVVFGF
                     IGARVYFIIPAILITFNLYSNVLIAKLKYILCKNKENDTNLKESEV"
     gene            3529..3981
                     /gene="accB"
                     /locus_tag="PC700_002619"
     CDS             3529..3981
                     /gene="accB"
                     /locus_tag="PC700_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDF2581917.1"
                     /translation="MNINEIKELLKAIDSTNLEYVKLESSDLRLEVSKKAQSTSSPVL
                     SVQQESVVDLSLEKPVVNDTPVTSNENLSVVVAPLMGTFYDSPSPDADSFVKVGDVVE
                     EGDTLCILEAMKLMNEITSEIKGEIIEVLVSNEELVEYNQPLFKIKPL"
     gene            3997..5358
                     /gene="accC"
                     /locus_tag="PC700_002620"
     CDS             3997..5358
                     /gene="accC"
                     /locus_tag="PC700_002620"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDF2581918.1"
                     /translation="MIKKILVANRGDIAVRIIRTCKELGIKTVAIYSEIDKDCFHRYI
                     ADESICIGPNNISKSYNNIENIIYLALKLKCDAIHPGFGFLSENPEFAKQCEDNNIIF
                     IGPTREQMILMGDKSRARETMMELNIPVVPGSESVLKTKEEALEVAREIGYPVMIKAS
                     SGGGGKGMRIVRKEEELFSNFDMASSEALAAFSNSDLYMEKFIENPRHIEVQVFGDKH
                     SNAIHLGDRDCSMQRRNQKVIEESLSPYLSDEERQKLHKIAVDIVKGVGYIGAGTIEF
                     IVDKDKNFYFIEMNTRIQVEHPVTEMVTNLDLIKLQISIANGDKIPFKQEDITFRGHA
                     IECRINAEDSSKNFAPSPGKIESLNLPGGFGVRFDTFVYAGYTIPPLYDSMIGKLICW
                     AETREECISRIYRALDEIIVEGINTNVEFQKALVTSEEFREDTHHTKFIEDVFMKKEF
                     ATL"
     gene            5373..6227
                     /locus_tag="PC700_002621"
     CDS             5373..6227
                     /locus_tag="PC700_002621"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HDF2581919.1"
                     /translation="MIKKFLSSKESKYVTISLDDNFKKNSVDDKFWTYCKGCDSHVFR
                     KDIEENSFVCPKCSRHYGLRARKRINLLIDKGTFMEFNSDVEFQNPLNFPKYKEKVDS
                     YKEKTKESEAVVTGYGRINGIKTVICVMNPDFMMGSMGSIVGEKITYSIEYAAENNLP
                     IIICSASGGARMQEGMVSLMQMAKTSQALSKLEEKSLPYISVLTDPTTGGVTASFAML
                     GDIIISEPNTLIGFAGPRVIEQTINQKLPEGFQTSEFLLEKGFIDMIVDRRKMKELLY
                     QILAMHKK"
     gene            6248..7192
                     /locus_tag="PC700_002622"
     CDS             6248..7192
                     /locus_tag="PC700_002622"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HDF2581920.1"
                     /translation="MIENNSEKIKVLENDIKQLITISKANNIDLSDKINSLNEKLEKL
                     KEDAFSHLSAYEKVTLSRDIKRPTTLEYIEHICSNFLELHGDRLFKDDPSMVGGIGQI
                     GKFNVTIVGHQKGRDTKENIKRNFGMPHPEGYRKALRLMKQAEKFNRPIITFIDTSGA
                     FCGLEAEERGQGEAIARNLLEMSKLSVPVITFVIGEGGSGGALGIGVGNDVCMLEHSV
                     YSVISPEGLSSILFKDSSKAKEACDVMKLTSNDLYDLKIIDKIIKEPLGGAQKDVEAV
                     SKEIKAYILERLNHYKDMDKEEIIAQRYKKFRNIGKCL"
     gene            7381..7641
                     /locus_tag="PC700_002623"
     CDS             7381..7641
                     /locus_tag="PC700_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HDF2581921.1"
                     /translation="MEVLKVSSKSNPNSVAGALAGVLRERGVAEIQAIGAGALNQAIK
                     SIAIARGFVAPSGMDLVCIPAFTDIEIEGDKKTAIKLIIEPR"
     gene            7794..8165
                     /locus_tag="PC700_002624"
     CDS             7794..8165
                     /locus_tag="PC700_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDF2581922.1"
                     /translation="MKLKLLVEEYAVCRLNNDSKIPTWIDTEKFYSITRTDDELSVVC
                     LNNNIPSDVKSEKEWRILKILGPLDFSLVGILSKLSSLLADNKISIFAISTYDTDYIL
                     VKEKDIEDACKILSCNGYEVE"
     gene            8195..9040
                     /locus_tag="PC700_002625"
     CDS             8195..9040
                     /locus_tag="PC700_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581923.1"
                     /translation="MIYIIEILKISVINIFCFIGIFIVFGLIFSITENLNNKFIFSSF
                     GKTGIIVTGAIGTFVHELSHLIMCLIFMHKINSVKFFRPIESKNDGILGYVSHSYKKD
                     NLYQSIGNFFIGIAPIIGGTIMIILIFKILLPDSYMKVTQNIDLKLYVSMINNLNING
                     FIHIILGDISNFIRIMLLTPSVYSLKYFIFMFLMYSISTHMSLSSADLKNSLNGLSFI
                     VIVIFVISSITYLFGFDNLNISSLIVKYNIFISFFMAIGFIFSIVTLIISYILSLVSP
                     FKNFS"
BASE COUNT         3545 a          954 c         1542 g         3083 t
ORIGIN      
        1 taaggtaata aaatattata taaaatattt taaagcctta ggtaatatta ggtagaagtt
       61 ttagtaaaat atgcaaaaac taattgatta tgtaccttgt atgggtatat aatagatatt
      121 gttttaataa aatatttgaa agggaatgat ttgatgaaaa ttactttacc agaaacagct
      181 attgatactt taaaaagtat cttaaaagac aatcaagata aacctaataa tataagagta
      241 tactttgcag gtgttggttg tggaggtcct tcttttggat tagctttaga tgagaaaaaa
      301 gaagatgact taacttatga agttggagaa ttacaatttg taatgagctc tgatgaatat
      361 agtcagtatg gagatataat aatagaagat acaggatttg gatttagagt tattcctgaa
      421 aatatgaaag atcaaggtgg aggaggatgc tctggttgtt cagggtgcca ctaagattaa
      481 gtttttagta agtttagaac tctctttata gaaataaatt tctattttaa gagagttctt
      541 tttatttttt ggtgagataa tgtataataa aaatgtattt tactaatttt ttatatgtaa
      601 ggagaattat tgtgagaaga agacgaaaaa aaggtgcaga tgaaaagctt ttaagttata
      661 caaaatatgt tttaagagac gatatagaca aattaaaagg caagtggaat ttaaagttta
      721 gaaatgataa tccaatacat gttgaatttg ggactggaaa aggtaagttt ataacaactt
      781 tagctaagca gaaccctgac ataaattata ttgccatgga acttaaggaa gaagttcttt
      841 taaaagctgt tgaaaaagca gatgcttcta atttgaataa tatactattt ttatggggag
      901 atgtaagcaa tattttggat tactttgaag ctaaagaatt atcgagaatt tatataaatt
      961 tctgtgaccc atggcctaaa aatagatgga gtaaaagaag actcactcat tctggatttt
     1021 tagaaatgta caatagggtt ttggaagatg atggcgaaat tcactttaaa acagataatg
     1081 aaaaattatt cgagtttagt ttaaatgaaa ttgctgcaaa taattggcta ttaaagaata
     1141 tttctcttga tttaggaaat agtgagtatg aaaataatgt tacaacagag tatgaagaca
     1201 aatttatgtc acaaggtatg cgaatattta gatgtgaagc taaaaaacgt aactagtaat
     1261 atgtttataa gtttaatagt agaaatttat tttaatttat attaataaag tgtacgaaag
     1321 taggtatata taacaaggaa acagaaccct ctcatagaaa ttaaattatt ataataaaaa
     1381 atttatgtgg gagggtacta tgaatttagc taatttaata ccatttatta tttctgcagc
     1441 agctttaagt ccaataaatt caaatgataa ggttttgagc gaaaatcaac ttaaagatat
     1501 ttttcctgat gcaaatttaa gagcagttgt aaaaagatat ataaacccag atgagatgac
     1561 tataagcaat ataaaggctt tagatggaga attttatgca actggtgaga gcatttcaaa
     1621 tttaaaagga atatcatatc ttgaaaatgt agacaatttt attttttgga ataataatat
     1681 aaaagaggtt cctaaggaag ctttaagtct aaaagatatg gattcaataa atcttgccaa
     1741 taattatttg attgatgacg atgtagtaaa ttctctttct cataatggtg tggatgtaaa
     1801 ttgcgattta aattttattg atacaaaaga taatcagtat aaactggact ctaaatatca
     1861 taatgttgat atagcaaaag gtgaaagtat tgatttgaga aaaataatta cgaagaaaat
     1921 tgatagttat tataaatact gggaagttac tgataatctt ccaaaagatt tagactttat
     1981 tgtatctgta agtgataaga gtgtactttc atgtgaagat atgataatta aaggtaataa
     2041 aacaggtcaa gctgtggtaa aagttatact aagcgacaaa aatgatttaa atacatctca
     2101 agaagttcta atcactgtca atgtaaaata atatgtcaac ttattcaatc attctatttt
     2161 caatttaagt gtataatcaa aataaaggtt gttagtaaca gaacgctaat attagtgctt
     2221 tgttactagc aacctttaaa ttttatagaa atagattatc taaaaaattc catttcttct
     2281 gttagtagcc catattccta ttaaaattat cagtgaatat attatataca aaactattct
     2341 tatatattgg acatctaatc caaaaaattc aatgttactc tgtgaggtat gcaacatcac
     2401 aaggactgca gatataccaa taagcaaagg attgattact atttgcttat cagttcttaa
     2461 aatcattatt ttacccatcc aaattattga taatataaaa aataaaattt taagaccaaa
     2521 ttctaatgta tctaccaaag cgaaaacatc ccttcattta taataaattt ttcagctcaa
     2581 caaaatataa aaccctacaa acaacttttg aatccataat aagataactt gattaagcac
     2641 tatgaaataa gtactataaa acttcatcta cattaatata tactacacta tacttcaaat
     2701 ttcttaaaca taaattaatg aaattaacaa aattttattc agaaacatat aaagatatat
     2761 tttactattt aaatgcatat cattatatat acagttttag gaggtgaggt atatggaatc
     2821 taaatttttt aattggtaca ctcaatcctt agggggaact tttggcatta tatcatgtat
     2881 atgcgcatat ttaaatggat acatgtttgt ttatacaaat agtgctagca aatattttga
     2941 aagtatagga tttctgggaa taatttcaag ttatacacta cttccacttt gtatgcttac
     3001 tttgattttt ggagttatat attcttatac tccagaaaca tccgacaaaa gtatttttaa
     3061 tatacctttt tttactttaa ataaaagatt aatacttatt acagtagttt ttggttttat
     3121 aggtgctaga gtttacttta taattcctgc aattctaata acttttaacc tatatagtaa
     3181 tgttttgata gctaaattaa agtacatcct atgtaaaaat aaagaaaatg atactaattt
     3241 gaaagaaagc gaagtctaaa ttgcataata ttggtatttg tcttaaaaat tcccgaggta
     3301 ttaatttgta ttctcttgta ttttgcaaaa aatattgaat tttattcatt ttatacttat
     3361 aatttatatt aataatggaa aattaaaaca aatataagtt actggttgtt aatttaaaat
     3421 taaaagatat attgatgtgt tgattctcta gtcagcactt tttcttttat aaatttgttt
     3481 tttgtgatat aatattagca ccaggtaata atacaagggg gtaacaaaat gaacatcaat
     3541 gaaattaagg aattattaaa ggccatagac tcaactaact tagaatatgt aaaacttgaa
     3601 agtagcgatt taagattaga agtatctaaa aaagctcaat ctacaagttc acctgtttta
     3661 agtgtacaac aagaatcagt agtggatctt tctttagaaa agccagttgt aaacgataca
     3721 cctgtcacat ctaatgagaa tctatcagta gtagtagctc ctttgatggg aacattctat
     3781 gattctccaa gtccagatgc agatagcttt gttaaagttg gcgatgtagt tgaagaaggc
     3841 gacactttat gtatattaga agcaatgaag cttatgaatg agataacaag cgaaattaaa
     3901 ggtgaaataa ttgaagtatt agttagtaat gaagaactag tagaatataa tcaaccttta
     3961 tttaagataa aaccattgta gtaggaggta tttaagttga taaaaaaaat actagttgca
     4021 aatagaggcg atatagcagt tagaatcatc agaacatgta aagaactagg tataaaaact
     4081 gttgctatct attctgaaat agataaagat tgttttcata gatatatagc tgatgaatct
     4141 atttgtatag gaccaaataa tataagtaag agttataata atatagaaaa tataatctat
     4201 cttgctctta aattaaagtg tgatgctatt catccaggct ttggattctt atcagagaat
     4261 cctgaatttg caaagcaatg tgaagataat aatattattt ttataggtcc aacaagagaa
     4321 caaatgattt tgatgggaga taaatctaga gcaagagaaa ctatgatgga attgaatatc
     4381 cctgttgttc ctggttcaga atctgttttg aaaactaagg aagaagcttt agaagttgca
     4441 agagaaattg gatatcctgt tatgataaaa gcctcaagtg gaggcggtgg aaaaggtatg
     4501 agaatcgtta gaaaagaaga agaacttttt tcaaattttg atatggcaag ttcagaagct
     4561 ctagcagcat tttctaatag tgatttatat atggaaaagt ttatagaaaa tcctagacat
     4621 atagaagtac aagtatttgg agataaacat tcaaatgcaa tacatttagg tgatagagat
     4681 tgttctatgc aaaggcgaaa tcaaaaagta atagaagaat ctttaagtcc atacctttca
     4741 gatgaagaaa gacaaaaact tcataaaatt gcagtagata tagtaaaagg agtaggttat
     4801 atcggtgcag gtactattga gtttatagtt gacaaagata aaaactttta ttttatagag
     4861 atgaatacaa gaatacaagt tgaacatcca gttacagaga tggtaacaaa tcttgacctt
     4921 ataaaacttc aaatatctat agctaatggg gataaaattc cttttaaaca agaagatatt
     4981 acttttagag gtcatgctat tgaatgtaga ataaatgcag aagattcatc aaagaatttt
     5041 gctccatcac caggtaagat agaatcatta aatttacctg gaggttttgg tgttagattt
     5101 gatacttttg tatatgcagg atatactata ccacctttat atgattctat gataggtaag
     5161 ttaatatgtt gggctgaaac tagagaagaa tgtataagta gaatatatag agctctagac
     5221 gaaattattg tagaaggtat taatacaaat gtagaatttc aaaaggcact tgtaacaagt
     5281 gaggagttta gagaagatac tcatcatacc aaatttatag aagatgtctt tatgaagaaa
     5341 gaatttgcta cattataagg agtaaataga ttatgataaa aaaattttta tcatcaaaag
     5401 aatctaagta tgttacgatt tctttagatg acaactttaa aaagaatagt gtagatgata
     5461 aattttggac ctattgcaaa ggttgtgata gtcatgtatt tagaaaggac atagaagaaa
     5521 attcatttgt atgtccaaaa tgttcaagac attatggctt aagagcaaga aaaagaataa
     5581 atttattgat tgataaagga acttttatgg agtttaattc tgatgtagaa tttcaaaatc
     5641 cactaaattt tcctaaatac aaagaaaaag tagactctta taaggaaaaa acaaaagagt
     5701 cagaagctgt tgtcacaggt tatggaagaa taaatggaat taaaacagtt atttgtgtaa
     5761 tgaatcctga ttttatgatg ggaagtatgg gttctattgt tggggaaaaa ataacttatt
     5821 caatagagta tgctgctgaa aataatttac ctataattat ttgttctgct tcaggaggag
     5881 ctagaatgca agaaggtatg gtatctctta tgcagatggc aaagacttct caagcacttt
     5941 ctaagcttga agaaaaatca ttgccatata tatcagttct aactgaccct actactggag
     6001 gagtaactgc tagttttgct atgcttggtg atattataat ttctgagcca aatacgttga
     6061 taggttttgc aggtccaaga gtaatagagc aaacaataaa tcaaaaactt cctgaaggat
     6121 ttcaaacttc tgagttttta cttgaaaaag gttttattga catgatagta gatagaagaa
     6181 aaatgaagga attattatat caaattcttg ctatgcacaa gaaatagggc ataaaggagg
     6241 aactgttttg attgaaaata atagtgaaaa aataaaagtc ttagaaaatg atattaaaca
     6301 attaataact atttctaagg caaataatat agatttaagt gataaaataa attcacttaa
     6361 tgagaaattg gaaaagctta aggaagatgc tttttcacat ctatcagcat atgaaaaggt
     6421 aacactaagt agagatataa agagacctac gacattggaa tacatagaac atatttgttc
     6481 taatttttta gaacttcatg gagataggct ttttaaagat gacccttcta tggttggagg
     6541 gattggtcag ataggaaagt ttaatgttac tatagttgga caccaaaaag gaagagatac
     6601 aaaagaaaat ataaaaagaa attttggaat gcctcatcca gaaggataca gaaaagcatt
     6661 aagacttatg aaacaagctg aaaaatttaa tagacctata ataacgttta ttgatacatc
     6721 aggagctttt tgtggtttag aagcagaaga aagaggacaa ggagaagcta ttgcaagaaa
     6781 cttacttgaa atgagtaaac tttctgttcc agtaatcact tttgtaattg gtgaaggtgg
     6841 tagtggagga gctttaggta tcggagtagg aaatgatgtt tgtatgctag aacattcagt
     6901 ttattctgtg atttctccag aagggctttc tagtattcta tttaaagatt catctaaagc
     6961 taaagaagct tgtgatgtta tgaagcttac aagtaatgat ttatatgatt taaaaataat
     7021 agataagatt ataaaagaac ctcttggagg agcacaaaaa gatgtagaag ctgtttcaaa
     7081 agaaataaaa gcttatattt tagaaagatt aaatcattac aaagatatgg ataaagagga
     7141 aattatagct caaagatata aaaaatttag aaatattgga aaatgcttat agaatataaa
     7201 gaaatttgta gttttttcat aataaaatgg ttaaaaaaaa taaaggtttt cttaaaacga
     7261 ttatagaagt atatttttat aaaagtatac tttaaatgat gattgtaatt agtaaatggc
     7321 aattatgatt tagtacttta ggtaatggat actttaacaa attaacaagg agatgttgaa
     7381 atggaggtac taaaagtttc atcaaaatct aatcctaatt ctgtagctgg agccttggct
     7441 ggagtactaa gagaacgagg cgttgcagaa atacaagcta taggagctgg agctctaaat
     7501 caagcgataa aatcaattgc aatagcaaga ggttttgttg cacctagtgg aatggattta
     7561 gtttgcattc ccgcatttac tgatatagaa atcgaaggtg ataagaaaac agccattaaa
     7621 cttataattg aacctagata atatatctct ttattagaat ttaattaagt tttaaaggtt
     7681 ggctatagaa ataggacact atagtgaacc ttttttactt tatatgtttt attttatgta
     7741 acttttaatg tataatctta agtaaacaaa taagattgga ggaaatatac attatgaaat
     7801 taaaattatt ggtagaagag tatgcagtat gtagattaaa taatgacagt aaaataccaa
     7861 catggataga tacagagaag ttttactcta taactagaac tgatgatgaa ttaagtgttg
     7921 tatgtttaaa taataatatt ccttctgatg ttaagtcaga aaaggagtgg agaatactta
     7981 agatactagg tcctttagac ttttcacttg taggaatttt atctaagtta agtagtttgt
     8041 tagcagacaa caaaataagc atatttgcaa tttctacgta tgatacagac tatatcttag
     8101 taaaagaaaa agatatagaa gatgcttgta aaattttaag ctgtaatgga tatgaagtag
     8161 agtagttaaa tacttttaca tgaggactta acttatgatt tatataatcg aaattttaaa
     8221 aataagtgta ataaatattt tttgtttcat aggaatattt attgtatttg gattgatttt
     8281 tagcattaca gaaaatctaa ataataaatt tatcttttca tcttttggaa aaactggaat
     8341 tattgtaact ggagctatag gaacgtttgt tcatgaattg agtcatttga taatgtgctt
     8401 gattttcatg cataaaataa attcagttaa gtttttcaga ccaatagaaa gtaaaaatga
     8461 tggtatacta ggatatgtaa gtcatagcta taaaaaagat aatctttatc aaagtatagg
     8521 aaatttcttt ataggaattg cacctattat aggtggaaca ataatgatta ttttaatttt
     8581 taagatatta ctgccagata gttatatgaa agtgactcaa aatatagatt taaagttata
     8641 tgtaagtatg attaataatt taaatataaa tggttttata cacattattt taggtgatat
     8701 ttccaatttt ataagaataa tgttgttaac acctagtgta tattcattga agtattttat
     8761 atttatgttt ttaatgtatt ctatatctac acatatgtca cttagtagtg ctgatttaaa
     8821 aaatagctta aatggtctga gctttatagt tatagttatt tttgtaataa gttcaattac
     8881 atacttattt ggatttgata acttgaatat atccagttta atagttaagt ataacatatt
     8941 tataagtttc tttatggcaa tagggtttat tttttcaata gtaactctca taatatccta
     9001 tattttaagc ttagtcagcc catttaaaaa ctttagttaa ataaatacat attttactaa
     9061 tatttggttt cgttagaaat atataaaaat attatataaa aaagatttta gtagagcata
     9121 aaaa
//
LOCUS       DAMJWP010000144         9073 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.149, whole genome shotgun sequence.
ACCESSION   DAMJWP010000144 DAMJWP010000000
VERSION     DAMJWP010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9073
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.149"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            258..914
                     /locus_tag="PC700_002626"
     CDS             258..914
                     /locus_tag="PC700_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581924.1"
                     /translation="MKKGDFIWSGVLICIIAVLAIPSSREVFMEATGAHPYIGGFLKF
                     AVLATMGDLLGARILYKDWKIPVGVFYRAIVWGIIGVVTTYGMSIYSSGVGQAQVDGM
                     LPFEGIAFANALFKSATMNILMSPTVFLFHKVMDCFIDKKYEVGKGIKVKLKDVVNIV
                     DWNAYLGFSVLKTIPFFWIPCHTIVFLFPAEYRVIASAFASIALGLILALANKSKVAV
                     "
     gene            complement(1384..2655)
                     /gene="sleC"
                     /locus_tag="PC700_002627"
     CDS             complement(1384..2655)
                     /gene="sleC"
                     /locus_tag="PC700_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic protein SleC"
                     /protein_id="HDF2581925.1"
                     /translation="MQDGFLTVSIIDATNNRPIQNAVVNIYSMSNGSQSSSTLYQNLR
                     SNESGQVTGLVLPAPDVDYSLQPSDVRPYSQYIVEAIADGYETVVIEGTQLLATIVAR
                     QGVPMSPRTRSKRSFSRQSELIFDIGEHTLYGTYPPKIPESNLKPLPPPTGFVVLDNP
                     VVPEFIVVHDGLPEDSSAPNYWIPFKEYIKNIASSEIYSTWPEQTIYANVIAIISFTL
                     NRVFTEWYRNKGYNFTITSTTAYDHKFINNRNLFEPINVVVDAIFNTFIKRPPTSRQP
                     LLAQYCDGQKSQCPDQMTQWGSKDLGDQGYDYESILRYFYGDEIVFERAPIVSGVPVS
                     FPGTTLQVGSSGQYVRTIQNQLNAISNSYPAVPKVIEDGIYGADTENAVKIFQGIFGL
                     PQSGVVDFKTWYEISRVYVATTRIASLNPLI"
     gene            2900..3616
                     /locus_tag="PC700_002628"
     CDS             2900..3616
                     /locus_tag="PC700_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HDF2581926.1"
                     /translation="MKLDLFLLAIVPILIGMFWIRSKDRYCREPLIHLIKFFLIGAFL
                     SVIIILLENLLMKFNVFEGYSELIYVSFVVAGLVEEGVKALILIPALIKEKHFTEKLD
                     GIIYSVFLALGFATIENMVYIFSESRNLALQVGINRAVISIPAHVMFAITMGYYISKY
                     KFEGNKNKRREYLFMAVLIPILLHGVFDFILMIEYRWAIILLIVYVIILWKINLDKLE
                     KYMNHSKKVFFGNLRKKKKK"
     gene            3695..5005
                     /locus_tag="PC700_002629"
     CDS             3695..5005
                     /locus_tag="PC700_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HDF2581927.1"
                     /translation="MTELPKEFLEDIEEILKDEYDDFIKSYEESKTTGLRINTLKIDK
                     KKLFDLELFDLNQIPWAEEGFYYDDKIDRPGRNPLHEAGAYYLQEPSAMSVIPKADIK
                     EGEKVLDMCAAPGGKSTYILSKLNDTGLLVSNEINASRIKALGENLERFGARNCIITN
                     TDSNSLRKTFNGYFDKVIIDAPCSGQGMFRKDEVAISDWSYAKVVECQSIQKEIIRDG
                     YKMLKKDGILIYSTCTFSREENEHVINEFISEYPHAELIEMERLWPHKIKGEGHFVAK
                     IKKLEEEECNVKELKIKNLDKEIKDYREFEKKFLKVDFYSNKKNKFYLRGENLYLLPE
                     IYPDTKKLKVLRYGLHLGVLKKNRFEPSHALSHYLKVEDVKNVENFSVQSESILKYLK
                     GDVVNSNDSRGWVLVSVEGIPLGWGKESSGVIKNHYPKGLRKVF"
     gene            5105..5641
                     /gene="lepB"
                     /locus_tag="PC700_002630"
     CDS             5105..5641
                     /gene="lepB"
                     /locus_tag="PC700_002630"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDF2581928.1"
                     /translation="MGEAVKKEVVEWIKVIVIALVLAFAITRFIVPTIVKGESMYPTL
                     VERDYLIVNRIAYKVGEPKYKDIIVFKTDLTEENGKKKDLVKRVIGVPGDHVKIQDSK
                     VYVNDKLLDETSYIHNNRTDGDIDIVVPEGKLFAMGDNREKSLDSRYDEVGLVDEHTI
                     LGKVLVRLYPFSKIGTID"
     gene            5807..6343
                     /gene="lepB"
                     /locus_tag="PC700_002631"
     CDS             5807..6343
                     /gene="lepB"
                     /locus_tag="PC700_002631"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDF2581929.1"
                     /translation="MNETIKEEIVEWIKIIITALFFAFIITRFIKPTLVNGESMYPTL
                     KSHDYLVANRMTYKLSEPKCGDIMIFKTDLLQENGRKKELVKRVIGVPGDHLKIKDSK
                     VYINGKLLNEVSYIHDNYTEGDIDMVIPKGKVFAMGDNREVSLDSRYKEVGLVDEENI
                     KGKVILRVFPFTDIGIFE"
     gene            6353..7108
                     /locus_tag="PC700_002632"
     CDS             6353..7108
                     /locus_tag="PC700_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HDF2581930.1"
                     /translation="MAVGIASLLFNIEEALNICESIKQITHLEIGIDNISECSELCKY
                     KERISKLGLSIGIHLPMELNTCENIEYIRNSWINFIEKIEFELKGFDIRYFNLHLGYV
                     MTNRVRKNRDKYLGNSVDFLDKLNTNSYVCIENTYSKGGDFSNIGNISYDFEYIFKRI
                     KNSKICFCYDTGHCLIDEDAYVKNLKDKIRLIHLSDNDGINDTHVGIGRGILSEEGIK
                     EVLTLDAEYLVLEINYEDIEDTISKLNNIVREG"
     gene            7182..8849
                     /locus_tag="PC700_002633"
     CDS             7182..8849
                     /locus_tag="PC700_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HDF2581931.1"
                     /translation="MKKINLKIEGKDKEYSLEENSPGIRLGDIAKEFCDEHKGYITLA
                     VVDNKLKELNCRVKKDCEINFLDTTNEDGERVYFRVMSFIFVMACREIFWDSRVTIEH
                     SLSDGLYCEVHIDRKLKEADVENIKNKMKEIVNNDYVIEKIEVTRNEAISIFENNEMY
                     EKAELLKYKEYDSAKVYKCRNYIDHFYGYMLPSTGYIKSFDIKLYNGGVIILGPSEED
                     KTLPMKFVPQPKLSSVYYEAEEWSRLMGIDKVVSLNKIIENNEYGDIIRTFEALHEKK
                     LSQIADMIKDGNKRVILIAAPSSSGKTSFAHRLSIHLRVNNLNPISISLDDYFIDRKH
                     TPLDEYGNYDFESIYAIDLEKFNLDLKKLLAGEEIDDIRFNFKEGKREYTGKKIKIDS
                     NQPIILEGIHGLNPILTSSIPDEDKFKIYISALTQINLDDHNRIPTADLRMIRRIVRD
                     YNFRGYSADNTILQWASVRRGEKKNIFPYQEEADAIFNSACVYELAVLKRFAKPLLEE
                     IKEDNPAHIEATRLLKFLQYFVELDDTSDIPGISILREFIGGSKIVD"
BASE COUNT         3394 a          943 c         1694 g         3042 t
ORIGIN      
        1 gatgatggta gataagaata tcagaaaaaa atagataatt aaagggttag ggtgaaagag
       61 taatcgtaaa ataaatatat agataatggt ggatagagta gtaagaaaat aaatagataa
      121 agggatagaa taaatttaaa tatggaaaat gaatatctaa taataagtga ttaataaata
      181 attgtagata gattgtaaat taaataaatc tttaatagaa aggagaacta tttatatatt
      241 aaatagtatt tataaatatg aaaaaaggcg attttatatg gagtggagtt cttatttgta
      301 ttattgctgt cttagcaata ccaagtagta gagaagtatt tatggaagca acaggtgcac
      361 atccatatat tgggggtttt ttaaagtttg ctgtattagc tacaatggga gatttacttg
      421 gtgctagaat tttgtataag gattggaaaa taccagtagg agtattttat agagcaatag
      481 tttggggaat aataggtgta gttaccacat atggaatgtc aatctactca tcaggtgtag
      541 gtcaagcaca agtggatggt atgcttccat ttgagggaat agcttttgca aatgctctat
      601 ttaagagtgc aactatgaat atactaatgt caccaactgt atttttattc cataaggtta
      661 tggactgttt tatagataaa aaatatgagg ttggaaaggg gattaaggta aaacttaaag
      721 atgtggtaaa tattgtggac tggaatgctt accttggttt tagtgtttta aaaactatac
      781 catttttctg gataccatgt catacaattg tattcttatt ccctgctgag tatagagtaa
      841 ttgcttctgc atttgcatct atagctttag gtctaatatt ggctttagct aataagtcta
      901 aagttgcagt ttaggttcac tgatgaagat aatagtatta tttaaattat gaataacttt
      961 ttaaattaat attatttata tttataataa ataataattt agataagaag gaatattcta
     1021 agtgatagat agtgttttaa ataataagga gtaatattct aagtgagaag tagcatttta
     1081 aataataagt gagattttaa ataaaaagta agcttttcgt tagaatagca ttttattaaa
     1141 cttaaaaata aatataatat tttaaagaaa tctaagttgc taagcactgt ttttaagaag
     1201 cttaaaatcc cccatttaaa gttttaagat tctaaaaaaa caagttataa ctaaaaaatg
     1261 gctgttaaga ctacaatact tataagtaaa aatacttatg gtatatatta gttcataaca
     1321 gccattttta ttttaaatta ataattttat tcaataattt gagtttatgt tctaaatcaa
     1381 gctttaaatt aaaggattta aagaagctat tctagttgta gctacataaa ctctggatat
     1441 ttcataccat gttttaaaat ctactactcc gctttgaggt aaaccaaaga ttccttggaa
     1501 tatcttaaca gcgttttctg tatctgctcc atagatacca tcttctataa cttttggtac
     1561 agctggataa gaattagata tagcatttaa ctgattttgt atggttctaa catactgccc
     1621 agaagaacca acttgtagtg tagtaccagg gaatgacact ggtacaccac ttactatagg
     1681 tgctctttca aatactatct catctccata gaaatatctt aggatactct catagtcata
     1741 tccttggtca cctaagtctt tactgcccca ttgagtcatt tggtcaggac attgagattt
     1801 ttgtccatca caatattgtg ctaatagtgg ctgtcttgat gtaggaggtc tttttataaa
     1861 agtattaaaa atagcatcta caactacgtt aattggttca aataagtttc tattatttat
     1921 aaacttatgg tcgtatgctg ttgttgaagt tattgtaaag ttatatcctt tatttctata
     1981 ccattcagta aagactctat ttaaagtaaa tgatattata gctattacat ttgcatagat
     2041 tgtctgttca ggccaagttg aatatatttc agatgacgct atgtttttaa tgtactcttt
     2101 aaatggaatc caatagtttg gagctgatga atcttctggc aaaccatcat gaactactat
     2161 aaattcaggc actacagggt tatctaatac aacgaaacca gtaggaggtg gtaatggttt
     2221 taagttactt tctggaatct taggaggata agtaccatat agagtatgtt ctcctatgtc
     2281 aaaaatcaac tcactttgtc ttgagaaaga acgcttactt cttgttctag gagacatggg
     2341 aactccttgt cttgctacaa ttgtagctaa caactgcgtt ccttctatga caactgtctc
     2401 atatccatct gctatggctt ccactatata ctggctataa ggtctaacat cagatggctg
     2461 tagagagtaa tctacatctg gtgctggtaa tactaaacca gtcacttgac cagattcatt
     2521 acttcttagg ttttggtata aagttgatga gctttggcta ccattagaca ttgaatatat
     2581 gtttactact gcattttgaa taggtctatt gttagtagca tcaattatgc ttactgttaa
     2641 gaaaccatct tgcatcaaat caccctttct ttaaatgaat ttagttccct tatatttcaa
     2701 gatatgaata tagcttgaaa tttgttaaat aatatagata taaatttaaa tttttttgag
     2761 tatttgagta tatttgtatt taaataattt aaaataattt aatgtatatt ttagtatgta
     2821 tttaaaacac aaataagcta aaaataagta gatgatataa atcataaata aatcaaaaat
     2881 tttgaaagga aaataatcta tgaagctaga tttattttta ttagcaatag ttcctatatt
     2941 gatagggatg ttttggataa ggtctaaaga tagatattgt agagaacccc taatccatct
     3001 aattaaattt tttttaatag gtgcttttct aagtgttata attatacttt tagaaaattt
     3061 actgatgaag tttaatgtat ttgaagggta ttcagaacta atatatgtat catttgtagt
     3121 tgcaggactt gtggaagaag gtgtaaaagc tctgatactg attcctgccc taattaaaga
     3181 aaaacacttt actgaaaagt tagatggtat tatttactct gtatttttag ctttaggatt
     3241 tgcaacaatt gaaaatatgg tttatatttt ttctgaaagt agaaatctag cactgcaagt
     3301 tggtattaat agggcagtta tctctatacc agctcatgta atgtttgcaa ttactatggg
     3361 ctactatatt tctaagtata aatttgaggg aaataaaaat aaaagacgtg agtatttgtt
     3421 tatggcagtg ttaattccaa ttttattaca tggagttttt gatttcatat tgatgataga
     3481 atatagatgg gctattatat tacttatagt ttatgtaata attttgtgga agataaatct
     3541 tgataaactt gagaaatata tgaatcactc taaaaaagtg tttttcggta atcttaggaa
     3601 aaagaagaag aaataaaatt taaaggaaag acataaataa ttaaagtata ataaatgaca
     3661 cacaagtaaa gcagattgaa gggggattat agatttgaca gaattaccaa aggaattttt
     3721 agaagatatt gaagaaatac taaaagatga atatgatgat tttataaaaa gttatgagga
     3781 aagtaagact actgggctta gaataaatac tttaaaaatt gacaagaaaa aattatttga
     3841 tttggagttg tttgatttga atcaaatacc atgggcagaa gaaggttttt attatgatga
     3901 taaaattgac agacctggta gaaatccact gcatgaggct ggagcatact atttgcaaga
     3961 gccttctgca atgagtgtaa taccaaaggc agatattaaa gagggtgaaa aagttctcga
     4021 tatgtgtgct gctcctggtg gaaaatctac atatatacta tctaagctaa acgatacagg
     4081 tttattggtt tctaatgaaa ttaatgctag tagaattaag gctctagggg aaaatttaga
     4141 gcgatttgga gcgagaaatt gtattataac aaacaccgat tctaatagct taagaaagac
     4201 gtttaatggg tattttgata aggttattat agatgcacct tgttctgggc aaggtatgtt
     4261 taggaaggat gaggttgcca tatcagattg gagttatgca aaggtagtag aatgtcaatc
     4321 catccaaaaa gagattatca gagatggata taaaatgtta aagaaggatg gcattttaat
     4381 ttactcaact tgtacttttt caagggagga aaatgagcat gttataaatg agtttatcag
     4441 tgaatatcca catgcagaac ttattgaaat ggaaagatta tggcctcata agataaaggg
     4501 agaaggtcat tttgttgcca aaataaaaaa attagaagaa gaagaatgta atgtcaaaga
     4561 attaaaaata aaaaatttag ataaagaaat aaaggattat agggagtttg aaaagaaatt
     4621 tttaaaggtt gatttttata gtaataaaaa aaataaattt tatttaagag gagaaaattt
     4681 gtacctactt cctgaaatat atccagatac gaaaaaacta aaagtattga gatacggttt
     4741 gcatttaggg gttttaaaga agaatagatt tgagccttct catgcactat ctcattactt
     4801 aaaagttgaa gatgtaaaaa atgttgaaaa cttcagtgta cagagtgaat caattcttaa
     4861 atatttaaaa ggggatgttg tgaattctaa tgatagtaga ggatgggttt tggtttcggt
     4921 tgaaggaata cctttaggtt ggggaaaaga atcttctggt gtaattaaga atcattatcc
     4981 taaaggtctt agaaaagtat tttaaactat tagaaaaaag aagtataata tttatattgt
     5041 aggtttatgg tagattattt tgctgtagat tgacttaata tttaacacag aggagacgat
     5101 tttagtgggt gaagcagtta aaaaagaagt tgtagaatgg ataaaagtga ttgtcatagc
     5161 tcttgttttg gcatttgcaa taactcgttt tatagtgcca acaatagtca aaggagaatc
     5221 aatgtatcct acattagttg aacgtgatta tttgatagtt aacagaattg cgtacaaggt
     5281 aggagagcca aaatacaaag atataatagt atttaaaacc gacttaacag aggaaaatgg
     5341 aaagaaaaaa gatttagtaa aaagagttat cggggttcct ggtgaccatg taaaaataca
     5401 agactccaag gtatatgtaa atgataagtt gttagatgag acttcctata tacataataa
     5461 tcgtactgat ggagatattg atatcgtagt tccagaagga aaattatttg caatgggaga
     5521 taatagagaa aaaagtttag atagtagata cgatgaggtt ggattggtcg acgagcatac
     5581 cattttagga aaggttctag tcagattgta tccattttct aagataggaa ctattgacta
     5641 aacatataaa atttggagca atcgctgatt agctgaaatc accagaccat acacaatagt
     5701 tgtctatata aaaaagacaa caccttattt agtagactta tctaaaagat agtgtttaag
     5761 agacattatc aataaagata atataactat aaaggagaat ttgtgaatga atgaaactat
     5821 taaagaagag attgtagagt ggataaaaat aattattact gcactttttt ttgcatttat
     5881 tataactcgt tttataaaac caacactagt aaatggagaa tcaatgtacc cgacacttaa
     5941 atcacatgat tatttggtag caaataggat gacatataag ttatcagaac caaaatgtgg
     6001 agatataatg atatttaaga ctgatttatt acaagagaat ggaaggaaaa aagagcttgt
     6061 aaaaagggtt ataggtgttc ctggtgacca tctaaaaatt aaggattcta aggtttatat
     6121 aaatggtaag ttattaaatg aagtttcata tatacatgat aattatactg aaggcgatat
     6181 tgatatggtg attcctaagg gaaaagtatt tgcgatggga gacaatagag aagttagttt
     6241 agacagtaga tataaagaag tgggattggt agatgaagaa aatattaaag gaaaagttat
     6301 tttaagagta tttcctttta cagatatagg tatttttgag taggtgattt gtatggcagt
     6361 tggtatagcg tccttattgt ttaatattga ggaggcttta aacatttgtg agagtataaa
     6421 gcaaataact catttagaga ttggaattga caatatatct gaatgtagcg agttatgtaa
     6481 atataaagag agaatttcta agttaggatt atctataggg atacacttgc ctatggaatt
     6541 aaatacttgc gaaaacatag agtatatacg aaattcatgg ataaatttta ttgagaagat
     6601 agaatttgaa ctaaaaggat ttgatataag gtattttaac cttcatttgg gatatgttat
     6661 gacaaataga gttagaaaga atagagacaa atatttagga aatagtgtgg attttttaga
     6721 taaattaaat acaaattcat atgtatgtat agagaatact tattcaaaag gtggggattt
     6781 ttcaaatatt ggaaatatat cttatgattt tgaatatata tttaagagga ttaaaaattc
     6841 taagatttgt ttttgttacg atactggaca ctgtttaatt gatgaggatg cttatgtaaa
     6901 gaacttaaag gataaaataa gactgataca cttaagtgat aatgatggta tcaatgatac
     6961 acatgttggt ataggaagag gaattttatc tgaagagggg attaaagagg tcttaacatt
     7021 agatgcagaa tatttagtat tagaaataaa ttacgaagac attgaagata ctataagtaa
     7081 attgaataat attgtaagag agggttaaat acctctcttt atcttgttga tatgcctcat
     7141 taactaaaat ttatattcat caaaaggaag gagatgttga agtgaaaaaa ataaacttaa
     7201 aaatagaagg aaaagataag gaatattcat tagaagaaaa ttcccctgga atcagattgg
     7261 gagatatagc gaaagaattt tgtgatgagc ataagggtta tataacactt gcagttgtag
     7321 acaataaact aaaagagcta aattgtagag ttaagaagga ttgtgagata aattttttag
     7381 acactactaa tgaagatgga gagagagttt actttagagt catgtcattt atatttgtaa
     7441 tggcttgtag agaaatcttt tgggatagca gagttacaat agaacattca ctatcagatg
     7501 ggctatattg tgaagttcat atagatagaa agcttaaaga agcagatgtt gaaaatataa
     7561 aaaacaagat gaaagagata gtaaacaacg attatgttat agaaaaaatt gaagttacaa
     7621 gaaatgaagc tatttctata tttgaaaata atgaaatgta tgaaaaggca gagcttttga
     7681 aatacaaaga gtatgatagt gctaaagtat ataagtgtag aaactatata gaccattttt
     7741 atggttatat gttgccatca actggttata taaaatcatt tgatattaag ctttataatg
     7801 gaggcgttat aattttaggg cctagtgaag aagataagac tcttcctatg aagtttgtac
     7861 ctcaacctaa gctatcaagt gtatattatg aagcagaaga atggtcaaga cttatgggaa
     7921 ttgacaaggt agtatctctt aacaaaataa tagaaaacaa tgaatatgga gatataataa
     7981 ggacattcga agcattgcat gaaaagaagt tatcccaaat agcggatatg ataaaggatg
     8041 gcaataagag agtaatttta atagcagctc catcttcatc tggaaaaaca agttttgctc
     8101 acagattatc aatacatctt agagtaaata atttaaatcc tatttcaatt tctttagatg
     8161 attattttat agatagaaaa catactcctt tagatgaata tggaaattat gattttgaat
     8221 caatttatgc aatagattta gagaagttta acttagactt aaagaaactt ttagctggtg
     8281 aagaaattga tgatataaga ttcaatttta aagaaggtaa gagagaatac actggtaaaa
     8341 aaataaagat agattcaaat cagccaatta tattagaagg tatacatggg ctaaatccta
     8401 ttttaacatc atctatacca gatgaagata aatttaagat atacataagt gcccttactc
     8461 aaataaattt ggatgaccat aatcgaatcc ctactgctga tttgagaatg ataagaagaa
     8521 ttgttagaga ctataatttt agaggatata gtgctgataa tactatttta caatgggcat
     8581 cagttagaag aggagaaaag aagaatatat tcccgtacca agaggaggca gatgctatat
     8641 ttaactctgc ttgtgtatat gaattagcag ttttaaaacg atttgcgaaa cctttgttgg
     8701 aagagataaa ggaagataat cctgcacata ttgaagctac tagattacta aagtttttgc
     8761 agtattttgt tgaattggac gatacttctg acataccagg tatttcaatt cttagggaat
     8821 ttattggagg aagtaaaata gttgattaat atgtgtatta tagattgttt tatgaagatt
     8881 aaatggcata gcaagtagtg tgattggttt atgttaagta tattaagtgt aaagtggaga
     8941 attaaattga tacagatttt aaagtttgag ttgatatata aaaaggcttg ttttatactt
     9001 ttgtatgaag caagcttttt attttaaatt ttactttttt gtttaatatt ttgttagttt
     9061 atgtgataat ttt
//
LOCUS       DAMJWP010000145         9046 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.150, whole genome shotgun sequence.
ACCESSION   DAMJWP010000145 DAMJWP010000000
VERSION     DAMJWP010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9046
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.150"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            1..261
                     /locus_tag="PC700_002634"
     CDS             1..261
                     /locus_tag="PC700_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581932.1"
                     /translation="MFKTVFEDKEYVLLCKNYSKSDGVKAIFIYLISMLLLFFQGYLY
                     TTNLSSTILTNSQMILSILILLIGLGFVLLSKEKLNTIGITM"
     gene            280..708
                     /locus_tag="PC700_002635"
     CDS             280..708
                     /locus_tag="PC700_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2581933.1"
                     /translation="MYGVIGATVLVIFVILTAGIMQGKKVDFQLPTVFMIVTFIVSAI
                     SEEIVFRGYIQTRLTGLIKNSVLSSCINAFLFLSTHYPVKWISSGDFSFAILSGFYVI
                     CLILLNFSCDLVYRKTNCLWGAWLLHILYNIGTGVLIFTT"
     gene            828..1436
                     /locus_tag="PC700_002636"
     CDS             828..1436
                     /locus_tag="PC700_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HDF2581934.1"
                     /translation="MVDNLIKYIHKSNIAIVVLHEIYGVNNFIKDVCQKYYKGGYDVF
                     CPELLGEKRVFSYLKAQEAYDFFINNVKFDIYKKVEKLINGLKSEYKHVFVVGYSIGA
                     TIAWRCSENSLCDGIICCYGSRIRDYMDVIPTCPILLVFAKYDSFDVDYVSSQLSRRQ
                     NIQIEILDANHGFIDTYSKNYSNAQTEVFNIKEKNFLSKCLG"
     gene            1960..2172
                     /locus_tag="PC700_002637"
     CDS             1960..2172
                     /locus_tag="PC700_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF2581935.1"
                     /translation="MMPLILSNTGEEVVIKKISGSDKTRTFLNSIGFVVGTNVKIVSK
                     IDGNLIIDVKDTRVALDKKMASRIII"
     gene            2185..2406
                     /locus_tag="PC700_002638"
     CDS             2185..2406
                     /locus_tag="PC700_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF2581936.1"
                     /translation="MNRLKDIKCGETVKVKKLEGEGATRRRIMDMGITRGVNIFIRKV
                     APLGDPIEVTVRDYELSIRKSDAEKIIVE"
     gene            2506..4662
                     /gene="feoB"
                     /locus_tag="PC700_002639"
     CDS             2506..4662
                     /gene="feoB"
                     /locus_tag="PC700_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDF2581937.1"
                     /translation="MSIKIGLIGNPNCGKTTMFNGLTGSSQYVGNWPGVTVEKKGGKL
                     KGNKDVEIVDLPGIYSLSPYTLEEVVTRNFMLDDKPDAVINIVDASNIERNLYLTTQV
                     LELGIPTIIALNMMDIVNKNGDKINIKELSEVIGCPVVEVTAVKGQGIMEAAEKAVEL
                     ASSNNKLNFKLPFVDESKDAIEKIEKIIEEKTPYIDVETRWLAIKLFERDENVIQKLD
                     ISKTILNSIEEITRNCEDELDDDSESIITANRYEFISSIISSIIKKNRKGKETVSDKI
                     DKIVTNRILALPIFALIMWGVYYIAVSSLGTIATDWTNDVLFGEIIQGNVSNFLASLN
                     VAEWLQGLVVDGLIGGVGAVLGFVPQIMLLFLLLSILEDCGYMSRVAFIMDRIFRKFG
                     LSGKSFIPMLISSGCGVPGVMSTRTIENDRDRKMTIMLTTFIPCGAKIPIIALFAGAL
                     FGGASWVAPSMYFLGIAMIIICGIILKKTSLFAGEPSPFVMELPQYHVPSAKGVLIHM
                     WDRGKAFIIKAGTIIFVACGVIWFLQSFNWSLQMVDAGDSILASLGNIVAPIFAPLGF
                     GNWQSSVATVTGLVAKENVVGTFGVLFGISDATEQDPTLLASVASMFTVASAFAFMAF
                     NMLCAPCFAAIGAIKREMGSWKWTWITLGFQTLTAYIIALLINQVGSLVLGTGGSIAG
                     AIISIFIAVAVVFVVLTYSNKNMKKEKMGKLSYMKN"
     gene            4688..4840
                     /locus_tag="PC700_002640"
     CDS             4688..4840
                     /locus_tag="PC700_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HDF2581938.1"
                     /translation="MATFIIAAIVVVLMALAVMYMVKNSKKNGSSCGCGCSGCSSSKS
                     CHSAKR"
     gene            5020..5730
                     /locus_tag="PC700_002641"
     CDS             5020..5730
                     /locus_tag="PC700_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HDF2581939.1"
                     /translation="MFKFDVTNELMCIARGSGKFFAKKGAMVAFKGNFNFEKLLLGPS
                     NGGGLGRALLGHVQRSLTGEQMPIMVVEGEGEIYLAQNAYHVDVISIDPGDSIYVESE
                     NLLAFSEQLNYSVKLIGSGVISQKGLFTTHLVNKTGQNQDVAIITDGNPLILEGPCCV
                     DPDALVAWTGREPYPKVSKLSWKTFIGQTSGESYHMEFVEPGQIVIIQPSERLSGLKT
                     DSTPSDRMSSGGLRLGID"
     gene            5815..6585
                     /locus_tag="PC700_002642"
     CDS             5815..6585
                     /locus_tag="PC700_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2581940.1"
                     /translation="MFEKIHKRFKGYYIFLIILILWKIVCVLGIWSSYILPPPEIVLD
                     TFIKMIYDGSIFLNVYASVRRILIGFLISSLMAIPLGVVFGVNKKMYEYFKPLFEFMR
                     STPPLALVPMLILWFGIGEESKIIIIILASFFPIFLNTLKGIKNCDNKLIEVGKSFNL
                     SKKRIFYKIIIPNSMLDIVVGLSLGLGYSFRAIIGAELIAASSGLGYLISDGKDMSRT
                     DVVLVGIFVIGFLGILSDYLFSKIITKFSKGKQVDLNG"
     gene            6578..7309
                     /locus_tag="PC700_002643"
     CDS             6578..7309
                     /locus_tag="PC700_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2581941.1"
                     /translation="MVRSGFLLENIYKKYLVDNKEHLVLDNISLNISSEEITVILGES
                     GCGKTTLLRILAGLENATSGNIYFFNNDKKCTPKVGMVFQESRLMPWLNVSENILLHT
                     EKDNRNKVDLDKYLKMMKLEKFKNSYPNELSGGMAHRVSIARALSFNPDILLMDEPFA
                     ALDYFTRRKMQKEVVNIHKNTKKGVVFVTHNIEEAMEIAKKIIVFSKNKRIKQFSVED
                     EYNRDLTKNYYINLKKEILRELGEF"
     gene            7343..8302
                     /locus_tag="PC700_002644"
     CDS             7343..8302
                     /locus_tag="PC700_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2581942.1"
                     /translation="MTSLIMVLVGTVGCSTTNDKKDEPAKKEASLPKEINLTYVKSPL
                     NVPSILEKKDDLFGKEFKKDNIKVNFHELTTGPEQTQALAAGELDFLHALGGTSAIIA
                     ASNGVGLKITNIYSRSPKGYMLITNNDNIKSIKDLKGKKIVGPKGTILHQLLIAALAK
                     EGYTIDDVEYVNMDIPSAASALESKSADVAMLAGPVALKTINSGAKMIVNGEGLVSGI
                     IVTAVSDDFAEKYPELVKRFMKVHEETLKYMNENKDEVMDVVSKEVGLSLDETKEMYS
                     WYDFSSKITDKDIKELEDTQEFLMSNGMQQKKINIKDMLYNQN"
     gene            complement(8400..8996)
                     /locus_tag="PC700_002645"
     CDS             complement(8400..8996)
                     /locus_tag="PC700_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HDF2581943.1"
                     /translation="MSCCNSQFCCKNKIESSYIKWLLEVLGPVFLGSKPAEIINISFT
                     DVNREEKINDIYKYLYKCKKIDFIVIDKEKKGLKILFVNKKALSEKLKCKKTVNFLKF
                     LGYKQNTNVNAYLEHLVNKLKSDVFPDEIGIFLGYPLKDVIGFMGYSNYEVSMIKYWK
                     VYGDTKQSEDTYSKFLLHRKKMRKLLDYISVDKIVSCF"
BASE COUNT         3370 a          961 c         1649 g         3066 t
ORIGIN      
        1 gtgtttaaaa ctgtatttga agataaagaa tatgttttat tatgtaagaa ttattctaaa
       61 agtgatggag taaaagcaat atttatttat ttgatatcta tgttactatt gtttttccaa
      121 ggttatttgt atacaacaaa tttaagttca acaattttaa ctaactcaca aatgatattg
      181 tcaatcttga ttttacttat agggctggga tttgtactac tttcgaagga aaaactaaat
      241 acgattggga ttacaatgtg aaatttaaat aaaagtcttt tgtatggagt tattggtgct
      301 actgtactcg taattttcgt tattttaaca gctggaatta tgcaaggaaa aaaagttgat
      361 tttcaattac ctactgtttt tatgattgtt acatttattg tttctgctat ttcagaagaa
      421 attgttttta gagggtatat tcaaacaagg ttaactggat taataaaaaa tagtgtactt
      481 tcatcttgta ttaatgcttt tctttttcta tctacacatt accctgtaaa atggatttca
      541 agtggagatt tttcatttgc aattctatct ggattttatg tgatatgcct tatattactt
      601 aacttttcat gtgatttagt atatcgaaaa actaactgtc tgtggggggc atggctactg
      661 catatcttgt ataacattgg cacaggagtg ctgattttta ctacttagtc aattttactt
      721 tgcagaaagt ttatcgtaag aaacggatta ttttataagt caaattttag agctagtaaa
      781 ttgacatact taaaataatt taaactagat gtaaaaaata tcgacaggtg gtggacaatc
      841 tgattaagta tatacataaa agtaatatag ctattgttgt actgcatgaa atttatggag
      901 ttaataattt tataaaagat gtttgtcaaa agtattataa aggtgggtat gatgtgtttt
      961 gtccagaact cttaggagaa aaaagagttt tttcatactt aaaagctcaa gaagcatatg
     1021 atttttttat aaataatgtt aagtttgata tttataaaaa agtagaaaaa cttataaatg
     1081 gtttaaaatc ggagtataaa catgtatttg tagttgggta tagtataggt gcaactattg
     1141 catggagatg tagtgaaaac tctttgtgtg atggtataat ttgttgttat ggctcccgaa
     1201 ttagagatta tatggatgta attccaacat gtcctatttt gctagtgttt gcaaaatacg
     1261 attcattcga tgttgattat gtttcttctc aactttctag aaggcaaaat attcaaattg
     1321 aaattttaga cgcaaatcat ggttttattg atacatactc aaaaaactat tctaatgctc
     1381 aaacagaagt ttttaatatt aaagaaaaaa actttctatc taaatgttta ggctaatatg
     1441 ttttattata aaatatagaa aatttaatca atttgaaaaa cataatgaca ctactaatgt
     1501 taaaaaatat tagtagtgtc attatatttt ttgagataga gatagtcagt agaatcatag
     1561 aattaattat gtttttatga ttgtattaga ttaatattta aattacattg aaaagaaaaa
     1621 aatcattact atagaataat atctttatca aaagaacatt cttatgcaga atatggttat
     1681 gagtatctaa gagaaatctc aaaatatata ggatagcagt atgaattttt atagatttta
     1741 ttaatacaat aaatcaaatg agaaaaaaaa tcaaaagaat acaataatca cttgcaaaaa
     1801 atatgctatt gtgatagact taaatcaaaa ttaggaattg taagaataag gaaaaagata
     1861 agtaattatt ttaacgagtc aaaatttaag aaattgtgat ttatatataa gatagaggtt
     1921 gtttgtaaat ttaaattggt tgaagaggag gaatattata tgatgccatt gatattatca
     1981 aatacaggtg aagaagtagt tataaagaaa atttcgggaa gtgataagac gaggactttt
     2041 ttaaatagta taggttttgt agtaggtaca aatgttaaaa tagtttctaa gatagatggt
     2101 aacttaataa tagacgtaaa agatactaga gttgcattag ataaaaaaat ggcaagtaga
     2161 ataataatat agggaggtaa tgacatgaac agactgaagg atattaagtg tggagagact
     2221 gtaaaagtca aaaagttaga aggagaagga gctacacgaa gacgtataat ggatatgggg
     2281 attacaagag gtgttaatat atttataaga aaagtagctc ctcttggtga tccaattgag
     2341 gtaacagtca gagattatga actatcaatt cgtaagtcag atgcagaaaa aattattgtg
     2401 gaataataaa gattaattag agtagggtta atgtaattat ataatgagaa tgattttctg
     2461 tatcacaaac agtgatgtat ttaaataata atggagggaa tggaaatgtc aataaaaata
     2521 ggtctcatag gaaatcctaa ctgtggtaag acaacaatgt tcaatgggct tacaggctcg
     2581 tcgcaatatg ttggtaactg gccaggagta acagttgaaa aaaaaggtgg aaaattaaaa
     2641 ggaaataaag atgtggaaat agtagattta ccaggtattt attctttatc tccatataca
     2701 ttagaagaag tagttacacg taattttatg ctagatgata agccggatgc agtaataaat
     2761 attgtagacg catcaaatat agagagaaat ttatatctta caactcaagt tttagagcta
     2821 ggtataccga ccattatagc tcttaatatg atggatattg taaataagaa tggtgataag
     2881 ataaatatta aagaactatc tgaagttata ggttgcccag tggtagaagt aacagcagtt
     2941 aaaggtcaag gaattatgga ggctgctgaa aaggcagttg aacttgcaag tagtaacaat
     3001 aaattaaatt tcaaattacc ttttgttgat gaaagtaagg acgcaataga aaaaattgaa
     3061 aaaataatag aagaaaagac tccatatata gatgtagaaa cacgctggtt agctataaag
     3121 ctatttgaga gagatgaaaa tgttatacaa aaacttgata tttctaaaac aatattgaat
     3181 agtattgaag aaataactag aaattgtgaa gatgaattgg atgatgatag tgaaagtata
     3241 ataacagcca atcgttatga atttattagt tcaataattt caagtataat aaagaagaat
     3301 cgaaaaggta aggaaactgt atcagataag atagataaga ttgtaacaaa tcgtatacta
     3361 gcattaccta tttttgcact tataatgtgg ggagtttact atatagcagt aagttcattg
     3421 ggtactatag caactgattg gacaaatgat gtactgtttg gagaaattat tcaaggcaat
     3481 gtaagtaatt ttctagcttc tttaaatgta gcagagtggt tacaaggact tgtagttgat
     3541 ggtctaattg gtggagttgg agcagtacta ggatttgtac cacaaatcat gcttttattc
     3601 ttattattgt caatattaga agattgtggg tatatgtcac gtgtagcttt tataatggat
     3661 agaattttcc gtaagtttgg tctttcagga aaatcattta taccaatgct tataagttca
     3721 ggttgtggag ttccaggagt tatgtcaact cgtactatag agaatgatag agatagaaaa
     3781 atgactatta tgttaactac attcattcca tgtggagcta agattccaat aatagcatta
     3841 tttgcagggg cattatttgg aggtgcttca tgggtagctc catcaatgta tttcttaggt
     3901 attgcaatga taatcatttg tggaattata ttaaagaaaa ctagtttatt tgcaggagaa
     3961 ccatctccat ttgttatgga attacctcaa tatcatgttc caagtgcaaa aggtgtttta
     4021 atacatatgt gggatagagg aaaagcattt ataatcaaag caggaacaat tatatttgtt
     4081 gcttgtggag taatctggtt cttacaatca tttaattggt cattacaaat ggtagatgct
     4141 ggagatagta tattagctag cttaggaaat atagttgcac caatatttgc tcctcttgga
     4201 tttggaaatt ggcaatcatc agtagcaaca gttacaggtc tagtagctaa ggaaaatgta
     4261 gttggaacat ttggagtttt atttggtata tcagatgcaa cagagcaaga cccaacactt
     4321 cttgcttcag ttgcaagtat gtttacagta gctagtgcgt ttgcatttat ggcatttaat
     4381 atgctttgtg caccttgttt tgcagctatt ggagccataa aaagagaaat gggttcatgg
     4441 aaatggacat ggattacatt aggattccaa actttaacag cgtatataat tgctcttctt
     4501 ataaatcaag ttggaagttt agttttaggt actggaggaa gtattgctgg ggcaataata
     4561 tctatattta tagcagtagc agtagtcttt gtagttttaa cttattcaaa taaaaatatg
     4621 aaaaaagaaa aaatgggtaa attaagttat atgaaaaatt aattcatata aataaggagt
     4681 gatgaatatg gctacattta ttatagcagc tattgtagta gtattaatgg cactagctgt
     4741 aatgtatatg gttaaaaatt caaaaaagaa tggtagcagt tgtggatgtg gttgttctgg
     4801 atgctcaagt agtaagagtt gtcatagcgc taagagataa ataggataag agcctgataa
     4861 aaagttaaat tgctttatta tcaggttctt attttttata taaataacaa atttcatatt
     4921 tgaagtaggt tagactatgc tgtaatttta ctgtataatt atatataaat atatgattag
     4981 gagggattat tgctaaatta agcaatataa gtgaatatta tgtttaaatt tgatgttaca
     5041 aacgagctta tgtgtatagc aaggggaagt ggtaaatttt ttgcaaaaaa aggtgctatg
     5101 gtagccttta aaggaaattt taattttgaa aaattgctgt taggtccaag taatggcggt
     5161 ggattgggaa gagcattatt aggtcatgtt cagaggtcat tgacaggaga acaaatgcca
     5221 ataatggtgg ttgaaggtga aggtgaaatt tacctagctc aaaatgctta ccatgtagat
     5281 gtaatatcaa ttgacccagg tgattccatt tatgttgaga gtgaaaactt attggcattt
     5341 tctgaacaat taaattacag tgtaaaattg attggttcag gggtaatatc tcaaaaaggt
     5401 ctttttacta cacatttagt aaataaaaca ggtcaaaatc aagatgttgc tataataaca
     5461 gatggtaatc ctctaattct tgaaggacct tgttgtgttg acccagatgc attagtagct
     5521 tggactggaa gagaaccata tcctaaagta tcaaaactat catggaagac ttttattggt
     5581 cagacttcag gagaatctta tcatatggaa tttgttgaac ctggacaaat agtcattata
     5641 caaccatctg aaagattgtc tggcttaaaa acagattcta caccttctga tagaatgagt
     5701 tcaggtggat taagacttgg catagattaa atcttatgat atagcttaat tattatttaa
     5761 gatattaaaa aataaaaagg gtagcaggga tagatatgtt aaaaggagtt ttatatgttt
     5821 gagaaaattc ataaaaggtt taaaggatat tatatttttt taataatttt aattttatgg
     5881 aagatagtat gtgttttggg tatatggagt agctatattc tgcctcctcc tgaaatagtc
     5941 ttagatactt ttataaaaat gatatatgac ggttctattt tcttaaatgt ttatgccagt
     6001 gtaagaagga tattaatagg ttttttaata agttcattga tggctatacc acttggagtt
     6061 gtatttggag taaataaaaa aatgtatgaa tatttcaaac cactatttga atttatgcga
     6121 agtacacctc cactagcttt ggtgccaatg cttattttat ggtttggtat aggtgaagaa
     6181 tctaaaatta taattattat tttggcatcc ttttttccaa tatttttaaa tacattgaaa
     6241 ggtataaaaa attgtgacaa taaattaata gaagtaggta aatcttttaa tttatctaaa
     6301 aagagaattt tttataaaat aataatccct aattctatgc tggatattgt tgttggactt
     6361 agtttagggc ttgggtatag ttttagggct ataattggag cagagcttat tgcagcttct
     6421 tcaggactag gatatttgat ttctgatgga aaagatatgt ctagaactga tgttgtattg
     6481 gtaggaattt ttgttattgg atttttaggt atactttcag attatctatt ttcaaagata
     6541 ataacaaaat ttagcaaagg gaagcaggtt gatttaaatg gttagaagtg gatttttgct
     6601 agagaatata tataagaaat atttagttga taataaagag catttagttc ttgataatat
     6661 atctttaaat atatctagtg aagagataac tgttatatta ggtgaaagtg gatgtgggaa
     6721 aacaacgcta cttagaattt tagcagggct tgaaaatgct actagtggaa acatatattt
     6781 ttttaataat gataaaaagt gtactccaaa agtaggaatg gtatttcaag aaagtagact
     6841 tatgccatgg ctgaatgtaa gtgaaaatat acttttacat actgaaaaag ataatagaaa
     6901 taaagttgat ttagacaagt atttaaaaat gatgaagctt gaaaagttta aaaactcata
     6961 tccaaatgaa ttatcaggag gaatggctca cagagtaagt atagcaagag cactttcctt
     7021 caatcccgat attttgctga tggacgaacc atttgcagcg ctagattact tcacaagaag
     7081 aaaaatgcaa aaagaagttg taaatatcca taaaaatact aaaaaaggtg tagtatttgt
     7141 aactcacaat attgaagaag ctatggaaat agcaaagaaa ataattgttt ttagtaaaaa
     7201 taaaaggatt aaacaattta gtgtcgaaga tgaatataat agagatttaa ctaaaaacta
     7261 ttatataaac ttgaaaaaag aaatattaag ggaattgggg gagttttaat tgattaaaag
     7321 aaacaaaaag attttagcag taatgacatc tttaataatg gtgttggttg ggactgtagg
     7381 atgttcaaca acaaatgata agaaagatga acctgcaaaa aaagaggcta gtttacctaa
     7441 ggaaataaat ttaacttatg taaaatcacc acttaatgtt cctagtatat tagaaaaaaa
     7501 ggatgattta tttggaaagg aatttaaaaa ggacaatata aaagtcaact tccatgaatt
     7561 gacaacagga ccagagcaaa ctcaagcatt agcagcaggt gagctagact ttttacatgc
     7621 attaggagga acgtctgcaa taatagctgc atcaaatggt gttggtctta aaattacgaa
     7681 tatttatagc cgttcaccaa aaggatatat gcttataact aataatgata atattaaatc
     7741 tataaaagac ttaaaaggta aaaaaattgt aggtccaaaa gggactatac ttcatcaact
     7801 gttgattgct gcacttgcta aagaaggcta tactatagat gatgttgaat atgtaaatat
     7861 ggatattcct agtgctgctt ctgcattaga aagtaaaagt gctgatgtag caatgcttgc
     7921 aggtccagtg gcattgaaaa cgataaattc aggtgccaaa atgattgtaa atggagaagg
     7981 attagtaagt ggaataatag ttactgctgt aagtgatgac tttgctgaaa aatatcctga
     8041 attagtaaaa agatttatga aggttcatga agaaacttta aaatatatga atgaaaacaa
     8101 ggatgaagtg atggatgttg tatctaaaga agttggttta tctttagatg aaacaaaaga
     8161 aatgtattct tggtatgact ttagttctaa aataacggac aaggatataa aagaattaga
     8221 agatacacag gaatttttga tgtctaatgg aatgcaacaa aagaaaataa atattaaaga
     8281 tatgttgtac aatcaaaatt agaatacttt aaaatattaa agtggaaaat acacccttat
     8341 ttattatgga aactgtatgg taaaagaggg tgtattttta ttattataca aaaatacaat
     8401 taaaaacaac ttactatttt atctacactt atgtagtcta gtaattttct cattttctta
     8461 cggtgaagta aaaactttga atatgtgtcc tcggattgtt ttgtgtcacc ataaactttc
     8521 caatatttaa tcatagaaac ttcataatta ctatatccca taaatccaat cacatcttta
     8581 agaggatatc ctaaaaaaat acctatttca tcaggaaaaa catcactctt aagtttattt
     8641 actaaatgct ctaaataagc attaacattc gtattttgtt tgtatcctaa aaactttaaa
     8701 aagtttactg tttttttaca ttttaatttt tctgataatg cttttttatt tacaaataag
     8761 atttttaatc cctttttttc tttatcaata actataaaat ctattttctt acatttatat
     8821 agatatttat aaatatcatt aattttttcc tctctattta catcagtaaa cgaaatattt
     8881 attatttcag caggtttaga gcctaaaaag actggaccta aaacctctaa aagccatttt
     8941 atatatgatg attcaatttt atttttacaa caaaattgtg aattacaaca agacataaaa
     9001 gataacctcc ttcaaatgaa aattaatatc aagtaattag attata
//
LOCUS       DAMJWP010000146         8899 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.151, whole genome shotgun sequence.
ACCESSION   DAMJWP010000146 DAMJWP010000000
VERSION     DAMJWP010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8899
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.151"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            74..415
                     /locus_tag="PC700_002646"
     CDS             74..415
                     /locus_tag="PC700_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HDF2581944.1"
                     /translation="MKEENFINVEYEDKRFSINSKEVLYIEKQEYEVKIYTESGNYEV
                     GENAPCIRKLLKEENFFKCSNSYIVNLDKCEFYLSNNCIFVNNNFIRILGSERKKKLK
                     KKLEKLYDYED"
     gene            566..979
                     /locus_tag="PC700_002647"
     CDS             566..979
                     /locus_tag="PC700_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HDF2581945.1"
                     /translation="MIELKNNLGISERKLKYPCIYKHFKGKYYATMGLSKAIDDIENI
                     CEIYGKENLIQNRNKYKLVIRHTEREEDIYVYRDLDGNLYHKKEEDTNDLVLYKTLYD
                     DTGIFARPLDMFLEKVDTDKYVNSIQEYRFEEVYK"
     gene            1181..1822
                     /locus_tag="PC700_002648"
     CDS             1181..1822
                     /locus_tag="PC700_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDF2581946.1"
                     /translation="MGNTFYIVRHGQTDWNILGKTQGHGNSDLTPQGIEQAKELSEDI
                     GKYSIDYIFSSDLGRAMQTAQILGDKLNIEVQKTEALREMGFGVWEGLLIKEIQKDYS
                     DIYATWRNEPHLVNIPEGETLKIIKERVDAFIKELNEKYDNKNIILVTHSITLRVMLL
                     SFLESGMENIYRIKQDNTALNIVEFKDYGPVIVKMNDTSHIKNHVKINNSALE"
     gene            1846..3099
                     /locus_tag="PC700_002649"
     CDS             1846..3099
                     /locus_tag="PC700_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF2581947.1"
                     /translation="MSKVIVVGGGASGMMAALSASKNNNEVILVERNGELGRKLRATG
                     GGRCNFTNNREIEDFFDKVVSNKKFLYSSFYTFTNKDLISYFESRNLEYKIEKENDHK
                     VYTKNDKSIEVIEVLNKDLLNHNVKIMYNKKVIDIITEEIALKDDSNKDKSKYLIKGI
                     ILDNGDKILGDKVIISTGGVSYSKTGSDGSMYKILKKHGHTLNKLYPALVPLTIEEKW
                     IKDLQGISMKNVEISCKIKKRKISKSGDMLFAHFGITGPCVLIMSSYINKIIEKEKVE
                     LNIDFLPNLSTDEISSIIRAFPNKNVLNNLKQILPQNFLREIFSLLSLVDKKASDLSK
                     ADEIRIIEYIKNMKLTCNGTTGINTGMVTSGGISVKEINSSTMESKLVKNLFFTGEVI
                     DIDAETGGYNLQIAFSTGYLAGISV"
     gene            3145..3795
                     /locus_tag="PC700_002650"
     CDS             3145..3795
                     /locus_tag="PC700_002650"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDF2581948.1"
                     /translation="MGNLVIAVDGPAGAGKSTIAKIVAKKLNINYIDTGAMYRAVTYK
                     CLKSGVDVNNEKEVIQIAENSDIDFKDNNIYLDKEVINEEIRTIEVSNNVSNVAKIKE
                     VRQLMVEVQRKIGMKSSVILDGRDIGSYVFPDADYKFFLVATPEERGNRRYKELCNKG
                     YNTTLEEVIEDIIRRDEIDSNREFAPLVKANDALEIDTTGKTIEEVVEEVVSKINL"
     gene            3846..4442
                     /locus_tag="PC700_002651"
     CDS             3846..4442
                     /locus_tag="PC700_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDF2581949.1"
                     /translation="MNFYRFVINIFKGFSKIFFKYEVIGAENIPDRGNIVIASNHKSN
                     LDPIFLAAAIENREIAAIAKKELFKVKPLGFILKKLHVMPINREKPDVSTIKTILRSV
                     RDGYVLGIFPEGTRIKGDSFGKAKAGLSVFTIKSKSKVVPVSIISKYKLFSKVIVYIG
                     EPISFEEHFKEKLSNDDHERISQEILEVIKQNYFKYSK"
     gene            4455..5294
                     /locus_tag="PC700_002652"
     CDS             4455..5294
                     /locus_tag="PC700_002652"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HDF2581950.1"
                     /translation="MNIKIAKNAGFCFGVKRAMKMAWDEVEKNDSGIYALGPLIHNKQ
                     AVAKYEEKGLKTVNEIDTIPNHENMIIRSHGVPENIYKEAKDKKLKIVDTTCPFVKKI
                     HTVVSEYHNKGYEIIVIGDMKHPEVIGINGWCENSAIIIKTLEQMENMEFNNSKKYCL
                     VAQTTINPELYISIVNKLSDKLEEIVFNDTICSATKTRQESAKELAKEVDCMIVIGGK
                     HSSNTQKLVKVCEDLVPTFAIETKDELDVNTLKKYKNLGITAGASTPNWIIEEVVTFL
                     ENL"
     gene            5539..6693
                     /locus_tag="PC700_002653"
     CDS             5539..6693
                     /locus_tag="PC700_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic efflux protein"
                     /protein_id="HDF2581951.1"
                     /translation="MEMLISASEEAFLHVGSMIGFFILLFGYINYKTSGNFTNIISKN
                     RKFQPLIGALIGAIPGCGGSLAIMPLYINGKLSFGAIIASLIASMGDAAFVLISSNIK
                     MYFFVTIVSTITGIITGQLVDYFKLEEKLGLKNRRKSDKYDISKNNPKENKEQHNHDE
                     IILDTLAKSHGNTNRLAFIITHGKGYKFYIGIIVIGFIFMSLAHSGLNLPIIEKLHSL
                     EEVIAVIGILFSIIYMWCFKKVFKNSNQKEEENKKISLREMLIHSVGEISFVITWIFI
                     AYLIYDLIILILGGDEYLVKLVLSTGVISVFIGAGLGLIPGCGIQIVLMSFYLKGTIP
                     LGAVIANSISQDGDALFPLLAMDKKSSLWAMIITTIPAILVGMIVYAFLG"
     gene            6999..8696
                     /gene="ade"
                     /locus_tag="PC700_002654"
     CDS             6999..8696
                     /gene="ade"
                     /locus_tag="PC700_002654"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HDF2581952.1"
                     /translation="MGNLKRQCDVSSGREKADIVLKNGTIINVFTEELITGDVAIVGD
                     TIVGIGDYKGNVEIDCSNKYISPGFIDAHMHIESTMVMPIELSKKLLKSGTTTIIADP
                     HELVNVKGASAIDFLLESTKDIPLNVYIMVPSSVPATSFETNGVGKFSAKDMESYVNN
                     PRILGLGEVMCFNDVINSENEILDKLELFKNKVVDGHAPNINGKSLQTYVCAGIENDH
                     ECITFDEVYEKLRAGLKILIREGSAAKNLKSIVSGMLKHNLPIEEFMFCTDDKHLDDI
                     EKQGHIRWNIKCAIDLGMEPVRAIKVATYNSAKAYGLRKIGAIGAGYKADIVVLNDLD
                     KMEVDSVYKDGNLVNEEMFSNYNYEIKDKELLNTVKFKYINKEKIQLKVSEKNYVMEI
                     VPYQILTNKVYESLPCADGYFVPNKEYSKLCVVERHRMTGNVAIASLKGFGIKNGAIA
                     TTVAHDSHNIIVAGDNDDDILVAINYLKEIQGGYVIVSNGKVLAHLSLQVAGLISTFT
                     AEEVQEITDNMLEIARKMGVPEYVDPFITLSFMALPVVPQIRLTDLGLFDVEEFKFI"
BASE COUNT         3639 a          859 c         1580 g         2821 t
ORIGIN      
        1 catatatttc cgatagatta tattgataaa aaacactaaa attaaattaa taaaagtact
       61 tggagggatg gaaatgaaag aggaaaattt tattaatgtt gaatacgagg ataaacgttt
      121 ttcaattaat agtaaagaag ttttgtacat agaaaagcaa gagtatgaag taaagattta
      181 tactgaaagt ggtaattatg aagttggaga aaatgctcct tgtataagaa aactattgaa
      241 agaagaaaat tttttcaaat gcagtaatag ttatattgta aatctagaca agtgtgaatt
      301 ttatctcagc aataattgta tttttgtgaa taataatttt ataagaattt taggttcaga
      361 aagaaaaaag aaattaaaaa agaaattaga aaaattatat gattatgaag attaatattg
      421 ggaggctgta ttttatacag cctctttatt tattgattgt attattaata aattttaaat
      481 tatgataaaa tataaaacaa gtaacttatt tcagaataaa atacttttta aaagaaaggg
      541 acgagatatg ggagattata ataaaatgat tgaattaaag aataacttag gtatcagtga
      601 aagaaagtta aaatatcctt gtatctataa acattttaaa gggaaatatt atgctacaat
      661 gggtcttagc aaggctattg atgatataga aaacatatgt gaaatatatg gtaaagaaaa
      721 tttaatccaa aacagaaata aatataaatt agttattaga catactgaaa gagaagaaga
      781 tatctatgtt tatagggatt tagatggaaa cctttatcat aaaaaagaag aagatactaa
      841 tgatttagtt ttatacaaga ccttatatga tgatacagga atatttgcaa gacctttgga
      901 tatgttttta gaaaaagtag atacagataa gtatgtcaat tcaattcaag aatacagatt
      961 tgaagaagta tataaataat ataagagaaa tatgtttata aatttagaaa aattctttaa
     1021 cagtacaata atttgcagaa atacaaatga gatttattgt aaataatatg ttagattaga
     1081 ttataaaata gaaacaaagt aaagtattat atgatataat taagttttga gaagtgataa
     1141 tttatattta ataaaaaata ggcatatatt aggaggacat atgggaaata ctttttatat
     1201 agtaagacat ggacaaacgg attggaatat attgggaaaa acacaaggtc atggtaattc
     1261 tgaccttacg cctcaaggta tagaacaggc aaaagaactt tctgaagata ttggcaagta
     1321 ttcaattgat tatattttta gtagcgacct tgggagagca atgcaaacag ctcaaatttt
     1381 aggagataag ctcaatattg aagttcaaaa aactgaggca cttagagaaa tgggattcgg
     1441 agtttgggaa ggccttttaa taaaagaaat tcaaaaagat tattcagata tatatgcaac
     1501 atggagaaat gaaccacact tagtaaatat accagaaggc gaaactctta agattataaa
     1561 ggaaagagta gatgctttta taaaggaact aaatgaaaaa tatgataata aaaatataat
     1621 tcttgttaca cattcaatta cattgagggt tatgttgttg tcatttttag aatcaggtat
     1681 ggaaaatata tatagaatta agcaagataa tactgcttta aatatagtag aatttaaaga
     1741 ttatggacct gttatagtaa aaatgaatga tacaagtcat ataaaaaatc atgtaaagat
     1801 aaataattct gcactggagt aggaatcaat tataaggaga aatttatgag taaagttata
     1861 gtagttggtg gaggagcttc tggaatgatg gcagctttgt cagcttcaaa aaacaataat
     1921 gaagtaatat tagttgagag aaatggagaa ttaggaagaa aacttagggc aactggtggc
     1981 ggtagatgca attttaccaa taatagagag atagaagatt tttttgataa agttgtcagt
     2041 aataaaaaat ttctctatag tagtttctat acatttacaa ataaagacct tatttcgtat
     2101 tttgaaagta gaaatttgga atataagatt gaaaaagaaa atgaccacaa agtatacact
     2161 aaaaatgata aatctataga agtaatagaa gtattaaata aagatttact caatcacaat
     2221 gtaaagataa tgtataataa gaaagttata gatattataa ctgaagaaat tgctctaaaa
     2281 gatgattcta ataaggacaa gtcaaagtat ctaataaaag ggattatttt agataatgga
     2341 gataagatac taggagacaa ggttattata tcaactggag gagtgagtta ttcaaagacg
     2401 ggttcagatg gttcaatgta taagattctt aaaaagcatg gacatacatt aaacaaacta
     2461 tatcctgctt tagttccact tacaatagag gaaaaatgga ttaaagattt acaaggaata
     2521 tccatgaaga atgtggaaat atcttgtaag attaagaaaa ggaaaatatc aaaatctgga
     2581 gacatgctat ttgcccattt tgggataaca ggaccttgtg ttcttataat gtcttcttat
     2641 attaacaaga ttattgaaaa ggaaaaggta gaattaaata tagatttctt accaaattta
     2701 agtactgatg aaatctctag tataatcaga gcatttccaa ataaaaatgt tctaaataat
     2761 cttaagcaaa tactacctca aaatttctta agagagattt tttctctatt atctttagtt
     2821 gataaaaagg caagtgattt gtctaaggca gatgaaatta gaattattga gtatataaaa
     2881 aatatgaagc taacttgtaa tgggactaca ggtataaaca ctggtatggt tacttctggt
     2941 ggtatttcag ttaaagaaat taattcttct actatggaat ctaaattagt taagaactta
     3001 ttttttacag gtgaagtaat tgatatagat gcagaaactg gtggatataa tttacaaata
     3061 gcattttcaa ctggatatct tgcgggtata agtgtataaa tataattttt taataattaa
     3121 atatgaataa atagaggtgt atatatgggt aatttagtta tagctgtaga tggtccagca
     3181 ggagctggaa aaagtacaat agccaagata gtagcaaaaa agctaaatat aaattatata
     3241 gatacaggtg ctatgtacag agcagttact tataagtgtt taaaaagtgg tgttgatgtc
     3301 aataatgaaa aagaagttat tcaaatagct gaaaattcgg atatagattt taaagataac
     3361 aatatatatt tggacaaaga agttataaat gaagaaataa gaacaataga agtaagcaat
     3421 aatgtttcta atgtagcaaa aataaaagaa gtaagacagt tgatggttga agttcaacga
     3481 aaaataggaa tgaaaagttc agtaatacta gatggtagag atataggttc atatgttttc
     3541 ccagatgcag attacaaatt ctttttagta gctactccag aagaaagagg aaatagacgc
     3601 tataaagaac tttgtaataa aggatataat actactttag aagaagtaat tgaggatata
     3661 ataagacgag atgaaattga ttcaaataga gaatttgcac cattggtaaa agctaatgat
     3721 gcactagaaa tagatactac aggaaaaact atagaagaag ttgtagaaga agttgtatct
     3781 aaaataaatc tttagttttt tatgcatcat aatgaaaata aatgtagtgt tgaagggaga
     3841 taaacgtgaa tttttataga tttgtaataa atatttttaa gggtttttct aaaatatttt
     3901 ttaaatatga agttataggt gcagagaata ttccagatag aggaaatata gtaatagcat
     3961 caaatcataa atcgaatctt gaccctatat tcttggctgc tgccatagaa aatagggaaa
     4021 tagctgcaat tgcaaagaaa gaattgttta aagtaaagcc attgggattt atattaaaaa
     4081 aattgcatgt tatgcctata aatagagaaa aaccagatgt atctacaata aaaactattt
     4141 taaggtcagt tagagatggt tatgtattag gaatatttcc agagggtact agaataaaag
     4201 gagattcttt tggtaaggct aaggcaggtc tttctgtatt tacaataaaa agtaaatcta
     4261 aagttgttcc agtatcaata atatctaaat ataaactttt tagtaaagta attgtatata
     4321 ttggagaacc aatatcattt gaagaacatt ttaaggaaaa attgagtaat gatgaccatg
     4381 aaaggatatc acaagagatt ttagaagtga taaaacaaaa ttactttaaa tattcaaaat
     4441 aagggggtaa agttatgaac ataaaaatag caaaaaatgc tggcttttgt tttggagtaa
     4501 aaagagcaat gaaaatggca tgggatgaag ttgaaaaaaa tgattctggc atatatgctt
     4561 taggtcctct tattcataac aagcaagcag tagcaaaata tgaagaaaaa ggtcttaaga
     4621 ctgtgaatga aattgataca ataccaaatc atgaaaatat gataataagg tctcatggag
     4681 ttcctgaaaa tatatataag gaagccaaag ataaaaaatt aaaaattgtg gatactacat
     4741 gtccatttgt aaagaaaata catacagtag taagtgaata tcataataaa ggctatgaaa
     4801 taatagttat tggagatatg aagcatccag aagttatagg tataaatgga tggtgtgaaa
     4861 attcagctat aataataaag accttagaac aaatggaaaa tatggaattt aataactcca
     4921 aaaaatattg tcttgttgca cagactacaa taaatccaga gttgtatata agtatagtta
     4981 ataaactatc agataaatta gaagaaatag tatttaatga tacaatttgt tcagctacaa
     5041 aaacaagaca agaatcagct aaagagttag ctaaagaagt tgattgtatg atagttatag
     5101 gaggtaaaca cagttcaaat actcaaaagt tagtaaaggt ttgtgaagac ttagtaccaa
     5161 cttttgcaat agagactaaa gatgaattgg atgtaaatac gcttaaaaaa tataaaaatt
     5221 tgggtattac agcaggggca tctacaccta attggataat agaagaagtt gtaacattcc
     5281 ttgaaaattt ataatttgat aggtaataaa aataggtgga cattgttata atttatcata
     5341 atatccacct ttgatttcat attacatgtg gtaaatagat aacctaatat caaaaagtgt
     5401 gtatcttaag atgaaaaact aagagtataa gatgcgcact ttttaatatt aaaaattcaa
     5461 aagaaatatt gatattgatt atcaatagaa aatagaatat aatataattg ttgcaaaatt
     5521 agtctaggag gtaaattaat ggaaatgttg atatccgctt ctgaagaggc gtttttacat
     5581 gtaggttcta tgattggatt ttttattttg ttatttggat atattaatta taaaactagt
     5641 ggaaatttta ctaatatcat atcaaaaaac agaaaatttc aacccttaat aggtgcttta
     5701 attggagcta taccaggttg tggcggaagt ctggcaataa tgccattgta tataaatgga
     5761 aaattaagct ttggagcaat aattgcaagt ttaatagcta gtatgggaga tgcagcattt
     5821 gtacttattt catcaaatat taaaatgtat ttttttgtca ctatagttag tacaattaca
     5881 ggaataataa cgggacaatt agtggattac tttaaactag aagaaaaatt agggttaaaa
     5941 aaccgtagaa aatcagataa atatgatata agtaaaaata accctaaaga aaataaagaa
     6001 caacataatc atgatgaaat aattttagac actctagcta aatctcatgg aaatacaaat
     6061 agacttgcat ttataataac acatggaaag ggatataaat tttatatagg aattatagta
     6121 atcggtttta tatttatgtc acttgctcac tctggactta atcttcctat aattgagaag
     6181 ttacattcat tggaagaagt aatagcagtg attggaatat tattttcaat aatttatatg
     6241 tggtgtttta aaaaggtatt taaaaattct aatcaaaaag aggaagaaaa taaaaagatt
     6301 tctttgcgag aaatgcttat ccattctgtt ggagaaatat catttgtaat tacatggatt
     6361 tttattgcat atcttattta cgatttgata atattaattt taggtggaga tgaatactta
     6421 gttaaactag tattatcaac aggtgtaata agtgtattta taggtgcagg tcttggactt
     6481 attccaggtt gtgggataca aattgtgctt atgtcatttt atttgaaggg aactatacca
     6541 cttggtgcag tgatagccaa ctctatttct caagatggag atgcactttt cccactttta
     6601 gcaatggata aaaaatcttc tttatgggct atgattatta ctactatacc agcaatactt
     6661 gtaggtatga tagtttatgc atttttaggt taaatataat tacgaataaa gaagtattaa
     6721 aaaaataata aagcacataa atttatttat aagaatagtg aagattaaat ataaaaataa
     6781 aaaattcaat atgaattaaa ttattgctta aatattaatg agaacatggg agataatatt
     6841 attcctcatg ttctctctat ttaattaaag gaattatgga acaaatttca agtgatactt
     6901 tctatcaaaa ctcatatata atatatatta ataaattaaa ctataatacg taatacagcc
     6961 agtatattga tataaaaaac ttaggaggag ttgtaaaaat gggtaattta aaaagacagt
     7021 gtgatgtttc aagtggaaga gaaaaagctg acattgtact aaaaaatggg acaatcataa
     7081 atgtatttac agaagagtta ataacaggag atgtagcaat agtaggagac actatcgttg
     7141 gcattggcga ttacaagggc aatgtagaaa ttgattgtag caataaatat atatcacctg
     7201 gctttattga tgcacatatg catattgaat ctacaatggt aatgccaatt gaattatcga
     7261 aaaaattatt aaaatcaggt acgacaacta tcatagccga cccacatgag ttggtcaatg
     7321 taaaaggagc tagtgcaata gattttttat tagaatcgac aaaagatata ccacttaatg
     7381 tatatattat ggtgccttca tccgtacctg caacaagttt tgaaactaat ggagttggta
     7441 aatttagtgc aaaagacatg gagagttatg taaataatcc tagaatttta ggtttaggag
     7501 aggtaatgtg ttttaatgat gttataaatt ctgaaaatga aatcttggat aaactagagc
     7561 tttttaaaaa taaagttgta gatggacatg cacctaatat aaatggtaaa tcacttcaga
     7621 catatgtatg tgcaggaata gaaaatgacc atgaatgtat aacttttgat gaagtatatg
     7681 aaaaattgag agcaggatta aaaattttaa taagagaagg aagcgcagca aaaaatttaa
     7741 agtctatagt aagtggtatg ctaaagcata acttgccaat tgaagaattt atgttttgca
     7801 cagatgataa gcacttagat gatatagaaa aacaaggtca cataagatgg aatataaaat
     7861 gtgcgattga tttaggtatg gaacctgtta gggctataaa agttgcaact tataattctg
     7921 caaaagcata tgggttaagg aaaattggag caataggtgc gggttataag gcagatattg
     7981 ttgtattgaa tgatttagac aaaatggaag tggatagtgt atataaagat ggtaatttgg
     8041 taaacgaaga gatgttttct aattacaatt atgagattaa agataaagaa ttgcttaata
     8101 cagtcaagtt taagtatatt aataaagaaa aaatacagtt aaaagtaagt gaaaaaaatt
     8161 atgtaatgga gattgtacct tatcaaattt tgacaaataa ggtatatgaa tctttaccat
     8221 gtgcagatgg atattttgtt ccaaataaag agtattcaaa actttgtgtt gttgagagac
     8281 atagaatgac tggaaatgta gcaattgctt cactaaaagg atttggcata aaaaatggag
     8341 caattgctac tactgtggcc catgattctc ataatattat agttgcaggg gataatgatg
     8401 atgatattct agtagctata aattacttaa aggaaataca aggtggttat gtaattgtat
     8461 caaatggaaa agttcttgca cacctaagtc ttcaagtagc tggattaatt agtactttta
     8521 cagctgaaga agtacaagaa attactgata atatgttgga gatagcaaga aaaatgggag
     8581 ttccagagta tgtagaccct tttattacac tttcttttat ggcacttcca gttgttccac
     8641 aaataagact tacagattta ggtctatttg atgttgagga atttaaattt atatagataa
     8701 tataaaagta ataataaatg aggctttctc aaaataggga taagtctctt tttattgttg
     8761 aaatgatgaa aatatatttg atttcataca aaaaagagca tctaatgaac taaaaatttt
     8821 atttttagat atccttattt taattaatct atcatttaac cttgttttaa gcatagatat
     8881 cttaataaaa ctattttca
//
LOCUS       DAMJWP010000147         8882 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.152, whole genome shotgun sequence.
ACCESSION   DAMJWP010000147 DAMJWP010000000
VERSION     DAMJWP010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8882
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.152"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            49..1341
                     /locus_tag="PC700_002655"
     CDS             49..1341
                     /locus_tag="PC700_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HDF2581953.1"
                     /translation="MREQFKNSIKNIKSNRPLVSVQDIVNFIDKGEKYLKLSIDEIDE
                     MEILQGIVMSPDYQRTYKSSNKEESSIIESLLIGIPIPEIFLVKIGVNDMQIRHVMDG
                     QHRLNAIYRYVKNKYPLKNLDILGNDPLYDNKRFSELEKKDKIKILGSHLSILEFDGF
                     DNPEIEIELFKRYNRNTKPLEMQEIEMATYFSETSKYISKFINTLIKDKYKDEIDKSE
                     YKYANYKSDLLRIYNITKSRNDKQKNHQEICIIFSILENGLQENIRDGVTASKKFLEY
                     KSIQYKNNESEKLELLKDKFDEFNRMILKISEKIEYPFSTSLISDDKQRQSKYLIGVS
                     IVLASICYYYDIDLENNYLIQDIKKIISCSPIADIEYKASSTNMKNMILYLFIKNKIQ
                     DNKFESLNLKRDKIKEINTLVMNNDGYLNEQTRLLEIY"
     gene            complement(1568..1753)
                     /locus_tag="PC700_002656"
     CDS             complement(1568..1753)
                     /locus_tag="PC700_002656"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDF2581954.1"
                     /translation="MEGFWRILKVEMYYLRKLDTYEQLVKTIEDYIYYYNNFRYQKRL
                     NSMSPLEFRKYLNTEIA"
     gene            complement(1783..2172)
                     /locus_tag="PC700_002657"
     CDS             complement(1783..2172)
                     /locus_tag="PC700_002657"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HDF2581955.1"
                     /translation="MRAVGLSSVCRKKKFSYVKCTPEVIAENVLSRKFSADKTSQKWL
                     TGVTEFKLTNGMKAYLSAILDLDDRSIVSYVIGKSNNNNLVLETFDKAIELYPNAKPI
                     FHNDKSFQYTSKVFKSKLLTQGMIQSM"
     gene            complement(2184..2644)
                     /locus_tag="PC700_002658"
                     /pseudo
     CDS             complement(2184..2644)
                     /locus_tag="PC700_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888992.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3073..4083
                     /locus_tag="PC700_002659"
     CDS             3073..4083
                     /locus_tag="PC700_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581956.1"
                     /translation="MITKIKKTYIYLKEIQKIYKINEHKELVHIIKKLINDNKIKPIK
                     TSDLTPQHEQLYTKYRIIKQETEEDKKILEEINYKICDKLSIDYYRKNLVHYKNNRVA
                     IMDLNSYLIKKSDNLSKKISINERSYEIFKDEKFISSKVGKEVLKNLKIDLIKDLNVY
                     KTPEPFIYLSINRISPQKILIIENKDTYITITKMLLEGKEILKNKIDTVIYGEGKKII
                     NSIKGLKEDITIEYLTNKENEFLYWGDIDKTGLSIYTSLKDTNEINNIRLFEVAYEHM
                     IDKTKENTLRNIPKNQKDNFECGLENINNLNLKESIKNILVQGKYIPQEALNRFDLEE
                     YN"
     gene            4083..5702
                     /locus_tag="PC700_002660"
     CDS             4083..5702
                     /locus_tag="PC700_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581957.1"
                     /translation="MEMINILEGYNKRVNCIGSYYLIATLANNRNKFKEFDNIQFYNL
                     LIQVLCYIFDRSLRRKNCLRDDIKDFIEEINRMDYKIMLSEDDLKDLANYIINGLTNS
                     GKVYLFTYYSLEQEKHIDESIKIIEDKNVKINNQERLSYSLTTEGYRLLLSTKEYDEL
                     FQIQISQMIAKLRIEKGDYQGAKEDIYDIINTLEIHQQKIDNYIKIIKVNILYIKEEK
                     YSTVIDTTVETLLEEMDKYEELKKEVKLLIKDKEDYLEKDEDIDKEKIKNIRTQLLQM
                     KELIDGIVLAKSTATNLLSRVQDFREEFKEILKKLLTTPVIKRFNFKEEILDRLEKDS
                     DFLLIIRELYISLFKVELPNRFNIGVAYQEQKLLNTREIENEIVNKDELEIIENKEKE
                     ERINYCENYYFDFFKYLIKYGLEKGQFTLEEFLKNYLKYDKVIYLEITKDSKLMRDII
                     IYFANQRESINIKKIIRMLENSAFSPNTDFQIERLIERLLVEDFELLNSVSHIKSNKI
                     FEDEMKINTEINLETMTAVKLEIANIKFEII"
     gene            5720..6448
                     /locus_tag="PC700_002661"
     CDS             5720..6448
                     /locus_tag="PC700_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581958.1"
                     /translation="MNISLNDYKIANEIFYRLLKKSELKEKEAKELFLAYINNSNVRY
                     ALEAICEVSKIKIILISDTLYLIPNNDNDIFGFDYRRESLLGKDLKDSYLGYLIMTII
                     FSEFTNGVAPASYIQVLNLLDLVQESLERSIARDNLEEIEKSCEFNITILKNYWESKR
                     KWDESSKKGIETTSADFQIGFIRKIINFLKNEGLIVHIEDEDKIKPTRRFLDLMNNYF
                     LDEERKVLIEKLVLRKGESINASN"
     gene            6435..>8882
                     /locus_tag="PC700_002662"
     CDS             6435..>8882
                     /locus_tag="PC700_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581959.1"
                     /translation="MPVINKYKIINFKFGMGQERILSNRTMELYGENLQVEAENTAGK
                     TMSIQTIIQSICPLSDVAKPFTAIYNKKEPLYSMTEWLLDDGKTLLLTGIGFEKKAGS
                     SKEEGKEKKDDLYKYFMFIIEESKEMEVDIDNIPLLKNNDKGIRIVQPLTASQDYIKM
                     LRDKYGKNKVHFFNNYLRKQHREKLEEYGINQSEWKEIIIKLNSNNKEGGLSDFVKEH
                     NTSEKLIRDKILPLIETSLTTEEFAPILQIKEQVRKCINNIIDMNEKLSDYKNYRDLN
                     IRVESLQNKINRILFIEEDKNNVLGELANLYVHIENKRNELNEEIKQYQKMKASKEEE
                     FKIVEYEEDSYEYFRKEKEIQDTQVEFDKAKNELNDKIEELELDEQYLNISKFKRKKK
                     GLEEGEKDKIEKESEKQRKEVNQEEITAIINRLGSSIHYCLKEESIINNEKLKENTNN
                     NIAIQEKKNQLNDDKSKLTKVLISKNSEIKYIEGKERDFNNQLHEFKLNNTDIKGFIS
                     NNFLGEVIDYTRYINYLNIKVKSIEENLESKVMQIEESNKLKDDINEAINNLKLDNHE
                     SYMKLQVLKTRKKDIDNFKEKYKIIKTRFEIDNELDNSTKLYKEVKQELNKMKLQVQD
                     LENNKKQLEKELEDLQNYKDIIFEEEILTDLERNGIKPIEGMKYLLTLDGDLEYKNNL
                     ISKNPLLPYSIIITNKEYQKLKQINLKNKTKISMPIFVRDNIEDTFITTSSNLVISNG
                     LNILSSFDNKALDPEKREKDMNLIKEQINSIRDKVEQYKAEIEILEEFKLLTNKIDFT
                     AEDLSIDVKIENYKKIIF"
BASE COUNT         3982 a          930 c         1241 g         2729 t
ORIGIN      
        1 taaatctata aaattctatt atgatgagat agtaggggga aatttataat gagagaacaa
       61 tttaaaaatt ctataaaaaa tattaaatca aacagacctc ttgtatcagt ccaagatatt
      121 gtgaatttta ttgataaagg agaaaagtac ttgaaattat caatagatga aatagatgaa
      181 atggaaattt tacaaggaat agttatgagt cctgattatc aacgtacata taaatcatca
      241 aacaaggaag aaagttctat aattgaatct cttttaattg gaataccaat acctgaaata
      301 tttcttgtaa aaataggtgt aaatgatatg caaataagac atgtaatgga tgggcagcat
      361 cgtttgaatg ctatatatag gtatgttaag aataaatatc cacttaaaaa tttagatata
      421 ttaggcaatg acccactata tgataataaa aggttttcag aattagagaa aaaagataaa
      481 attaaaatat taggaagtca tttatcaatt ctagaatttg atggttttga taatccagaa
      541 atagaaatag aattatttaa aagatataac agaaatacaa aaccattaga gatgcaagaa
      601 atcgagatgg caacatattt ttcagaaact agtaagtata taagtaaatt tataaatact
      661 ctaataaaag ataaatataa ggatgaaata gataaatcag aatataaata tgctaattac
      721 aaatcagatt tacttagaat atataacata actaaatctc gtaatgataa acaaaaaaat
      781 catcaagaaa tatgtataat atttagtatt ttagaaaatg ggctacaaga aaatatcaga
      841 gatggagtta cagcctctaa gaaattttta gagtataagt caattcagta taaaaataat
      901 gaaagtgaaa aattagagtt attaaaagat aagtttgatg agtttaatag gatgatactg
      961 aaaatatctg agaagataga atatccattc tcaacaagtt taatatctga tgataagcaa
     1021 agacaaagta aatacttgat aggagtatct attgtgttgg cttctatttg ttattattat
     1081 gatatagact tggaaaataa ttatttaata caagatatta aaaaaataat atcttgttca
     1141 cccatagcag atatagaata taaggcgtct tcaactaata tgaaaaatat gattctatac
     1201 ttatttataa agaataaaat acaagataat aaatttgaat cattaaattt aaaaagagat
     1261 aaaattaaag aaataaatac tttagtaatg aataatgatg gttatttaaa tgaacagaca
     1321 aggctattag aaatatatta acaagttata aaataaatca tggtaatgta aaataatttg
     1381 tgcttttaag gttaataaaa atcttcttta tggcaattat ttaaatataa tcaaatgtcg
     1441 taaaggagat tttttatgaa cgctttaaga aaacaattag ccaaagaaat tatatcagat
     1501 atctaaaaaa gagcatgtca aataaaaccc taggtattta aattttttta gagctactta
     1561 aaataaatta tgctatctct gtatttaaat atttcctaaa ttcaagtgga gacatagaat
     1621 ttaatctctt ttgatatcta aaattattat agtaatatat ataatcctct attgttttga
     1681 ctaattgttc gtatgtatca agttttctta aatagtacat ttccactttt aaaattctcc
     1741 aaaatccttc cataggagca ttatcaatac aacgagcaac tcttacatac tttgtatcat
     1801 gccttgtgtt aaaagttttg atttaaatac cttactagta tactggaaac ttttatcatt
     1861 atgaaaaata ggttttgcat ttggatatag ctctattgct ttatcaaaag tttctaacac
     1921 caagttattg ttatttgact ttccaataac atatgaaact atacttctat cgtctaaatc
     1981 tagaattgcg cttaaatagg ccttcatccc attggttaat ttaaattcag ttacacctgt
     2041 taaccatttt tgagaggttt tatctgcact aaattttcta cttaatacat tttccgcgat
     2101 cacttctggc gtacatttaa cataactaaa tttctttttt ctgcaaacag aacttaagcc
     2161 tacggctctc attagtctgt acactctcta caatctttaa caatttcaac tctttcatct
     2221 aatgctgttt ttctagtctc cataaagatt tctcccttcg aggtttcttt tatctactta
     2281 tgaggattat actctttacc ccaagctttt agtgaataaa ctgatctcat tttatatttt
     2341 tttgagattg tgtcataaga tccttcacca tttagataag attttatagc attcaattta
     2401 aattcttcac aatagcataa tttacaactt tgttttatta aaccatcatt tccaaaagct
     2461 tcataatttc gcatccactc tctgaatgta cgtttatcca atatttaatt cttccattat
     2521 ttttattact gtattctcac cttttaaata tgattcaatt gcttcgattt ttttgctctg
     2581 aagatatctt agttttagat agtcttacat attttaactg tctcctaaga tgagtataat
     2641 tcattcatca tcagttctat agaatacacc tagttcctac ttactgtagg aactaggact
     2701 atatgaaacc cattcataga ttgtagtttc ttctgttgca ttatagcgca gaatagtaaa
     2761 ttcatgtgaa attttatttt gaatttacta tttatatata aatgaagagt ggtcttattt
     2821 tcaattaaaa tttggaacaa ttttgggttg acatctacaa ttaaggattt aagtttttgt
     2881 tatactaata aataaaaaca gagattcaaa atatataaat aatacttaaa ctagtgctga
     2941 tagattttct ttaagttcta gtttttttat tgtcattatc taatatgtac tattttatgt
     3001 acagtcaata taatctgaaa ttgtaaaatg gtgtataata tagttataag taaagaaggg
     3061 atttaaacat gtatgattac aaaaataaaa aagacttata tataccttaa ggaaatacaa
     3121 aaaatataca aaatcaatga gcataaagaa ctagtacaca taataaaaaa actaataaat
     3181 gataataaaa taaaaccaat aaaaacctca gatctaacac ctcagcatga gcaattatat
     3241 acaaagtata gaataataaa acaagaaaca gaagaagata aaaaaatatt agaagaaata
     3301 aattacaaaa tatgtgataa attaagtata gattattata gaaaaaactt agtacactat
     3361 aaaaataata gagttgcaat aatggattta aatagttatc ttattaaaaa atctgataat
     3421 ctatcaaaga aaatatcgat taacgaaaga tcttatgaaa tatttaagga tgagaaattc
     3481 ataagtagta aagtaggaaa agaagtacta aaaaacttaa aaatagatct aataaaggat
     3541 ttaaacgtat ataagacacc agagccattc atatatttat caataaatcg tatatctcca
     3601 caaaaaatat taattataga aaataaagac acttacataa caataacaaa aatgctatta
     3661 gaaggaaaag aaatacttaa aaataaaata gatactgtta tatacggaga aggaaaaaaa
     3721 ataatcaaca gtattaaagg attaaaggaa gatataacaa ttgaatatct tacaaataaa
     3781 gaaaatgaat ttttatattg gggagatata gataaaactg gattatcaat atacacttcc
     3841 ctaaaagata ccaatgaaat taataatata agattatttg aagttgctta tgagcatatg
     3901 atagataaaa caaaggaaaa taccttgagg aatatcccca aaaatcaaaa agataatttt
     3961 gaatgtggat tagaaaatat taataaccta aacttaaaag aaagtattaa aaatatttta
     4021 gtacaaggaa aatacatacc tcaagaagct ctaaatagat tcgacttgga ggaatataac
     4081 taatggaaat gattaatatt ttagaaggct acaataagag agtaaactgt ataggatctt
     4141 attatcttat tgcaactctt gctaacaata gaaataaatt taaagaattt gacaacattc
     4201 aattttacaa tttattaatt caagttttgt gttatatatt tgatagatct cttagaagaa
     4261 agaactgttt aagagatgat attaaggatt ttattgaaga aataaatcgt atggattata
     4321 aaataatgct ttcagaggat gacttaaagg atttagcaaa ctatataatt aatggattaa
     4381 ccaatagtgg taaagtatat ctatttacct actattcttt agaacaagaa aaacatatag
     4441 atgaaagtat taaaattata gaagataaaa atgtaaaaat aaataatcag gaaagactat
     4501 cctactcctt aacaacagag ggttatagat tgttgttatc aacaaaagaa tatgatgaac
     4561 tattccaaat tcaaatatca caaatgattg caaagttaag gatagaaaag ggtgattatc
     4621 aaggcgcaaa ggaagatatt tacgatataa taaatacctt agagattcat caacagaaaa
     4681 ttgataatta tataaaaata ataaaagtaa atatactata tataaaagaa gaaaaatatt
     4741 caacagtaat agatacaact gtagaaacac ttttagaaga aatggacaaa tatgaagaat
     4801 taaaaaaaga agtaaaacta ttaatcaaag ataaagagga ctacttggaa aaggatgagg
     4861 atatagacaa agaaaagata aaaaatataa gaactcaact attacaaatg aaagaattaa
     4921 tagatggaat agttttagca aagtctacag ccacaaattt gttatctaga gttcaagatt
     4981 ttagagaaga atttaaagaa atacttaaaa aactattaac aactccagta attaaaagat
     5041 tcaatttcaa ggaagaaatc ttagatagac ttgaaaaaga cagtgatttt ttattgataa
     5101 taagagaatt atatatatct ttatttaaag tagagttgcc aaatcgattt aatattggag
     5161 tggcatatca agaacagaaa ctattaaata ctagagaaat tgaaaatgaa atagttaata
     5221 aagacgagtt agaaattatt gaaaataaag agaaggagga acgtataaat tattgtgaaa
     5281 attactattt tgattttttt aaatacttaa ttaaatatgg acttgaaaaa ggacaattta
     5341 cattagaaga atttctaaaa aattatctga aatatgacaa agttatttac ttagaaataa
     5401 ctaaagactc caaattgatg agagatataa taatttactt cgctaatcaa agggaaagta
     5461 tcaatatcaa aaagataatc agaatgttag aaaatagtgc cttctcacca aatacagatt
     5521 ttcaaattga aagattaata gaacgtttat tagtagaaga ttttgaacta ttaaatagcg
     5581 ttagccatat taaaagtaat aaaatatttg aagatgaaat gaaaataaat actgaaataa
     5641 atttagaaac tatgactgca gttaagctag aaatagcaaa tataaaattt gaaataatct
     5701 aaatataagg ggaaatacaa tgaatatatc tttaaatgac tataaaatag ccaatgaaat
     5761 attttataga ttacttaaaa aaagtgagtt aaaagaaaaa gaagctaaag aattattttt
     5821 agcttatata aataacagta atgtaaggta tgctttagag gcaatttgtg aagtatctaa
     5881 aataaaaata atattaattt cagatacctt atacttaata ccaaataatg acaatgatat
     5941 ttttgggttt gactatagaa gagaatcttt attagggaaa gatttaaaag atagttattt
     6001 aggatatttg ataatgacaa taatattttc tgaatttaca aatggggtag caccagcatc
     6061 atatattcaa gtattaaact tactggattt agtacaagaa tccttagaac gttcaatagc
     6121 tcgtgataat ctagaagaaa tagaaaaatc atgtgagttt aatataacta tcttaaagaa
     6181 ctattgggaa agtaaaagaa agtgggacga atctagtaag aaaggaatag aaacaacatc
     6241 agcagatttc caaatagggt ttattagaaa aataattaat ttcttaaaaa atgaaggctt
     6301 aattgtacac attgaggatg aagataagat aaaaccaact agaagatttc ttgatcttat
     6361 gaataattat tttttagatg aagaaaggaa agtattaata gaaaaactag ttttaagaaa
     6421 aggggaaagt attaatgcca gtaattaata aatacaaaat tataaatttt aaatttggta
     6481 tgggacaaga aagaatactt agcaacagaa ccatggaact gtatggagaa aacttacaag
     6541 tagaagcaga aaatacagca ggcaaaacta tgagtattca aactatcata caaagtattt
     6601 gtccactatc ggatgtagcc aaaccgttta ctgcaatata taataaaaaa gaaccattat
     6661 actcaatgac tgaatggttg ctagatgatg gtaaaacgtt attattaacg ggaataggat
     6721 ttgagaaaaa ggctggatca tctaaagaag aagggaaaga aaaaaaggac gatttataca
     6781 aatactttat gtttattata gaagaatcta aagaaatgga ggttgatata gataatatac
     6841 ctttattaaa aaacaatgac aaaggtataa ggatagtaca acctttaacg gcctcacaag
     6901 actatataaa aatgctaaga gataaatatg gaaaaaacaa agtacacttc tttaataatt
     6961 acttaagaaa acaacataga gaaaaattag aagaatatgg aattaaccaa agtgaatgga
     7021 aagaaattat tataaaactt aatagtaata acaaagaagg tggactatca gactttgtta
     7081 aagaacataa tacttcagaa aaacttataa gagataaaat attaccttta atagagacaa
     7141 gcttaacaac agaagaattc gctccaatat tgcaaattaa ggaacaagta agaaagtgta
     7201 taaataatat tatagacatg aatgaaaaat tatcagatta taaaaactat agagatttaa
     7261 acattagagt tgaaagttta caaaataaaa taaataggat cctattcata gaagaagata
     7321 aaaataatgt attaggagaa ttagctaatc tatatgtaca tatagaaaat aaacgcaatg
     7381 aattaaatga agaaataaaa cagtatcaaa aaatgaaagc atcaaaagaa gaggaattta
     7441 agatagtaga atatgaagaa gatagctatg agtattttag aaaagaaaaa gagatacaag
     7501 atactcaggt agaatttgat aaagccaaaa atgaattaaa tgataaaata gaagaactag
     7561 aattagatga acaatattta aatatatcaa agttcaaaag aaaaaagaag gggctagaag
     7621 aaggggaaaa agataagata gaaaaagaaa gtgaaaaaca aagaaaagag gtaaatcaag
     7681 aagaaataac ggctattata aatcgtctag gtagcagtat tcattattgt ttaaaggaag
     7741 aaagtattat aaataatgaa aaacttaaag aaaatacaaa caacaatata gctatacaag
     7801 aaaaaaagaa tcaacttaat gatgacaaaa gtaagctcac aaaagtattg ataagtaaaa
     7861 attcagaaat taaatatata gaaggcaaag aacgtgattt taataatcaa ctacatgaat
     7921 ttaaacttaa taacactgat attaaaggct ttatttctaa taatttttta ggcgaagtaa
     7981 tagattatac taggtatata aattatttaa atataaaagt aaaatcaata gaagaaaatc
     8041 ttgaatcgaa agtcatgcaa atagaagaat cgaataaact taaggatgat ataaatgaag
     8101 ctataaataa tcttaaatta gataatcatg aatcttatat gaaactgcaa gtattaaaaa
     8161 ctagaaagaa agatatagat aattttaaag aaaaatataa aatcattaaa acaagatttg
     8221 aaatagacaa tgaattagat aactctacta aactatataa agaggtaaaa caagagctta
     8281 acaaaatgaa acttcaagta caggacttag aaaataataa aaagcaatta gaaaaagagc
     8341 tagaagattt acaaaattat aaagatataa ttttcgaaga agaaatatta acagatctag
     8401 aaagaaatgg aataaaacct attgaaggca tgaaatatct attaacctta gatggagatt
     8461 tagaatacaa aaataatctc atatctaaaa atccattgct accatattct ataataatta
     8521 caaataaaga gtatcaaaaa ctgaagcaaa taaacttaaa aaataaaact aaaatctcaa
     8581 tgcctatatt tgtaagagat aacatagaag atacttttat aactacatct agtaaccttg
     8641 taattagtaa tggattaaat atactatctt ctttcgataa caaagcttta gatcctgaaa
     8701 aaagagaaaa ggatatgaat ctaatcaaag aacaaataaa tagtattcga gataaagtgg
     8761 agcaatataa agcagaaatt gaaatattag aagaatttaa acttctaact aataaaattg
     8821 attttacagc tgaagactta tccatagatg tgaaaattga aaattataaa aaaattatat
     8881 tt
//
LOCUS       DAMJWP010000148         8745 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.153, whole genome shotgun sequence.
ACCESSION   DAMJWP010000148 DAMJWP010000000
VERSION     DAMJWP010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8745
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.153"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            158..1219
                     /locus_tag="PC700_002663"
     CDS             158..1219
                     /locus_tag="PC700_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HDF2581960.1"
                     /translation="MEKRKSIHLHKLNIYLIGGGDRLFITVVLFLVGFLLITKGADIF
                     INCTVEIGRKTNISEIILGATIVSFATTLPEFTVSLLASIDGHTTMSLGNAVGSIICN
                     TGLALGLVVFISPFNVDKKMFFSKSLLLIVSVIVLILLSLDGVITRGDSLLLIIILIF
                     YMVNNYRSVVGKSDTKNRNIKNNTIKDISTKKGKNYRGFSVLEIVKILLLFATGLIMM
                     IIGSQILIESGVIIASFLNIPQGIVSLTIIALGTSLPEIVSSITAIRKNHHEISVGNI
                     LGANILNIVSVIAVSAIPNNIPILSQNRQLDIPFMILLLLIVIIPTLKSNRLSRIQGI
                     LMLFTYFLYISILYFMYII"
     gene            1310..2923
                     /locus_tag="PC700_002664"
     CDS             1310..2923
                     /locus_tag="PC700_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2581961.1"
                     /translation="MNITKFEDLPISEGIKKAIAEMGFEEPSPIQAQSIPAILSGKDV
                     IGQAQTGTGKTAAFSIPILETIDPNNRSLQAVVLCPTRELAIQVSTEIRKLAKYSHGI
                     KTLPIYGGQPIDRQIKSLKSGVQVVIGTPGRTIDHINRKTLKMDNVKMIILDEADEML
                     DMGFREDIEMILSKIPEERQTTFFSATMPRGILELTKRYQKDPEHIKVVRKELTVSNT
                     KQYYIETRSSNKLEVLCRLVDVYDPKLSVVFCNTKRKADELVGDLQARGYFADALHGD
                     LKQTQRDIVMDKFRNGTIDILVATDVAARGIDVDDVECVFNYDLPQDEEYYVHRIGRT
                     GRAGREGMSFTFVFGKEMRKMKDIERYTKSKLIKHNIPTITDVEEKKVGTFFAQVKQT
                     IEEGHLTKQLQWLEGFCNDEDYAMVDIAAALVKLSLGEEMKEEIIEEKPRRERGDRKG
                     GTGAKDGMIRLFINIGRNQRVQAKDIVGAIAGEVGIPGKVVGTIDIYDKYTFVEIPKK
                     DAKTVIEKMKDIKIKGNKINIEKANKKKK"
     gene            3198..5183
                     /locus_tag="PC700_002665"
     CDS             3198..5183
                     /locus_tag="PC700_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDF2581962.1"
                     /translation="MSMTYMDPRVFLILEMVLEEDTNTLEQFSNVLGVSTRTIRNYIN
                     QINRELKGGIAKIVKNEKGTYSIRIEDESKLIEIVNFNRGKLSNFINLNSPDERINYV
                     LDILTMTNNPITIDDLAEEICIGRTTLIKDLKKADRILAEYDLELKRKPNIGMILVGE
                     EMDIRLLILDRLYENYIDVLENVNQINLVKDYDIECLRDELKKLFKKEELYITEQVLR
                     DVERYIIMSVLRNLNGYKFEKIDKRFEVIRCSDEYTLGLKLKALLEKIFNIVLNEKET
                     IFLTMPLIGRKAPSTKLALSSIKINDSVKEVVDEVTSFLLETSGIDFKEDKKLIENLE
                     YHLYFALNRMRFNIRVKNPLLQEIKERYTFPYSVAKIAAMIIEEKFDLKICDHEIGYI
                     ALHFGSYFEKNSRKVLNVNRVAVVCGTGLGTAQLIRIKIGKILGESVEVNTFSDIEAK
                     ANLDLLKYYDLVFTTVDFKTDLDVPIFRVNALFNEESIKKEIENAILLKNSINLKSIK
                     KIEKNIPFIGKLIEEDKFFILNKSTFMENLEDMLEKLVHKGIIDIEFKKKVLKREKKS
                     STALDNYIALPHSVNTNGENLYLSFGILEKPVVWDTKEIRIIILMIIPDKNVDSTDLI
                     IKSYEEVLEVGRDKKMIEELAKVKSFDEFYKIITKRR"
     gene            5188..5622
                     /locus_tag="PC700_002666"
     CDS             5188..5622
                     /locus_tag="PC700_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HDF2581963.1"
                     /translation="MDPVIFIIASLITVYLLNIFLGYLQLKDFNKNYIELKRKGRVAI
                     GRKKGRISSGTIVLILINDDGVIVETRKMQGVTVLARVKSFEGLVGKSLEYIEKSDLT
                     VYNKLLKKAILDAVKQYITFEKNKCEDTNNLSTDEECRMEVV"
     gene            5651..6211
                     /gene="srlA"
                     /locus_tag="PC700_002667"
     CDS             5651..6211
                     /gene="srlA"
                     /locus_tag="PC700_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418408.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDF2581964.1"
                     /translation="MTQFFQVLASGADSFMNLFRAGGQQFVGFVTDIVPLLVALLVTM
                     NAIINFIGTDRVERLAKKCSANIFTRYLILPVLGTFVFANPMTLSLGRFLPEKFKPSY
                     FAAASFSCHTMNGLFPHINPGELFIYLGIANGITTLGFSTADLAVRYLLVGIVANFIK
                     GVVTDYTTKFVEKQQGIALNSDIKIG"
     gene            6275..6643
                     /locus_tag="PC700_002668"
     CDS             6275..6643
                     /locus_tag="PC700_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIB"
                     /protein_id="HDF2581965.1"
                     /translation="MAFNKVKIVKGSGGWGGPLIIEPTEKKNKVVYVTGGAKPETAVK
                     IAELTGCELIDGFSQGVKDDEIACVIINCGGTLRCGIYPQKKIPTINIMKTGKSGPLA
                     MFIKEDIYVSGVKPTNVEQL"
     gene            6668..7234
                     /locus_tag="PC700_002669"
     CDS             6668..7234
                     /locus_tag="PC700_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDF2581966.1"
                     /translation="MAYDTKKKISEQSQGIVAKIGIALGNVTSTLFQAGRDTIDTLIH
                     TILPFMAFVAVLIGIINQSGIGNWVANLLTPLIGNVGGLVVLSIICSFPLLSPFLGPG
                     AVIAQIVGVLVGTEIGKGNIPPSLALPALFAINSQAACDFIPVGLGLAEAEPDTVQVG
                     VPSVLYSRFLTGAPTVLLAWLASFGLYK"
     gene            7275..7640
                     /locus_tag="PC700_002670"
     CDS             7275..7640
                     /locus_tag="PC700_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDF2581967.1"
                     /translation="MELIYSTQINKIGDNANEFLQHNMFVTFKDNAPQELENYCYIHS
                     ENNLVKEILTNDVLSINNVDYKITSVGELVNQNLSELGHITFKFTGEKDGAIGGTLYL
                     EEKEIAPIGVGTILKIIRK"
     gene            7741..8514
                     /gene="srlD"
                     /locus_tag="PC700_002671"
     CDS             7741..8514
                     /gene="srlD"
                     /locus_tag="PC700_002671"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HDF2581968.1"
                     /translation="MRKVALVVGGGRSLGEYLSKHLAKVGYDVAIADLNEDNAKKVAE
                     EIETEFGCKSVGYGCNAIDERQVISTVKAIENDFDRIDLLVYNAGVAVSKKITDFGYK
                     DFKFIVDVNLNGYFLFAREVSKVMIAKGIEGRIIQINSKSGRVGSKHNSGYSAGKFAG
                     VGLTQSLALDLAEHKITVNALMLGNLLDSDMFESLIPQYAVKLGIPESEVKQVYIDKV
                     PLKRGCRFIDVANMLTFYASKEAEYCTGQSINITGGQVM"
BASE COUNT         3493 a          886 c         1621 g         2745 t
ORIGIN      
        1 cataaaaaat aaaaaattgt cgctttaaag tataaaacag attatatgtt aataatgtgg
       61 tgaaattata cccatatata aataaaataa aacaaataaa tagctaatta aggagaaaat
      121 attttttatt ttttgtttag gataaataaa ttttattatg gaaaaaagaa aaagcataca
      181 tcttcacaaa ttgaatattt atttaatagg gggaggtgat agattgttta tcacagtggt
      241 attattttta gtaggtttcc tattaattac taaaggcgca gatatcttta taaattgcac
      301 tgtagagata ggtagaaaaa cgaatatatc agaaattata cttggagcta ctatagttag
      361 ttttgcaaca acattacctg aatttacagt gtctctactt gcttctattg atggtcatac
      421 tactatgagt ttaggaaatg cagttggttc tataatatgt aatacaggat tagcattagg
      481 gttggttgtt tttataagtc catttaatgt agataaaaaa atgttttttt ctaagtcttt
      541 actattaata gtatccgtta tagttttaat tttattgagt ttagatggag ttataacaag
      601 aggtgattca ttattactta taataatatt aatcttttat atggttaata attatagaag
      661 tgtcgttgga aaatctgata ctaaaaatag aaatattaaa aataacacta ttaaagacat
      721 atctactaaa aaagggaaaa attatagagg tttttccgta ttagaaattg taaaaatact
      781 attacttttt gctacaggat tgataatgat gataataggt tcacaaatcc ttatagagag
      841 tggagtaata atagctagtt ttttaaatat acctcagggt atagtaagtt taactataat
      901 agcacttgga acttctctgc cagagatagt ttcgtcgatt actgctataa gaaaaaatca
      961 tcatgaaata tcagtaggaa atattttggg tgcaaacata ttgaatatag tatcagtaat
     1021 agctgtatca gcaataccaa ataacatacc aatactatct caaaatagac aattggatat
     1081 accattcatg atattattat tattaatagt aataatacca acacttaaat ctaatagact
     1141 tagtagaata caaggtatat tgatgttatt cacatatttt ctgtacatat caattttgta
     1201 tttcatgtat attatttaac tatactgttt aaaatattaa ttgcagaata tcctaaaaaa
     1261 gtatataatg gataggttaa gattaattga taacggagag gaataatata tgaatataac
     1321 aaagtttgaa gatttaccaa taagtgaggg aataaaaaaa gctatagctg aaatggggtt
     1381 tgaagaacca tctcctatac aagcacagtc tatacctgcg attttgtcag gtaaagacgt
     1441 tataggacaa gctcaaactg gtacgggaaa aacagctgct tttagtatcc ctatactaga
     1501 aacaatagac ccaaataata gaagtttaca agctgtagtg ctttgtccaa caagagaact
     1561 ggcaatacaa gtatcaacag aaataagaaa gttagctaag tattcgcacg gaataaagac
     1621 attacctata tatggtggcc agccaataga tagacaaata aaatctctaa agagtggtgt
     1681 gcaagttgta ataggtacac caggacgtac aatagaccat atcaatcgta aaacattaaa
     1741 gatggataat gtaaaaatga ttatattaga tgaagcagat gaaatgctag atatgggatt
     1801 tagagaagat atagaaatga ttttaagtaa gattccagaa gaaagacaaa caactttctt
     1861 ttcagctact atgccaagag gaattttgga attaactaag agatatcaaa aagacccaga
     1921 gcatataaaa gttgttagaa aagaacttac agtttcaaat acaaagcaat actatataga
     1981 aactaggtca tcaaataaat tagaagtatt atgtagatta gtagatgtat atgaccctaa
     2041 actatctgtt gttttctgta atactaagag aaaagcagat gaattggtag gagatttaca
     2101 agcaagagga tattttgcag atgcattaca tggtgactta aaacagactc aaagagatat
     2161 agtaatggat aagtttagaa atggtacaat agatatactt gttgctactg atgttgcagc
     2221 tagaggtata gatgttgatg atgttgaatg tgtattcaac tatgacttac cacaagatga
     2281 agagtattac gttcatagaa ttggtagaac tggtcgtgct ggtagagaag gtatgtcatt
     2341 tacttttgta tttggaaaag aaatgagaaa gatgaaagat atagaaagat atactaaatc
     2401 taaactaata aagcataata taccaacaat aactgatgtt gaagagaaaa aagtaggtac
     2461 tttctttgca caagttaaac aaactataga agaaggtcac ttaactaaac aattacaatg
     2521 gttagaaggt ttctgtaatg atgaagatta tgcaatggtt gatatagctg ctgcacttgt
     2581 aaaactatct ctaggtgaag aaatgaaaga agaaataata gaagaaaaac ctagaagaga
     2641 gcgtggagat agaaaaggtg gtactggagc taaagatggt atgattagat tatttatcaa
     2701 tattggtaga aatcaaagag tacaagcaaa agatatagtt ggtgctatag ctggagaagt
     2761 tggaatacca ggtaaagtag taggtactat agatatatat gataagtata cttttgttga
     2821 aatacctaaa aaagatgcaa aaacagtaat tgaaaagatg aaagatatta agataaaagg
     2881 taataagatt aatatagaaa aagcaaataa gaagaaaaaa taacgtatag tatacaaaac
     2941 tataattaga aaatatataa aataatatat tggatattta aaaagcctga tgtgttttca
     3001 aaacagatag tagataatct gtaagagtta acagcataag gcttttcttg ttttatatga
     3061 tattatgatt ttccagatcg ttgtgaaaaa tgtatattag ttcctttttt tcaaatctga
     3121 tttgaaaaag agaatattaa ttatctatat agtttagatt ataataaaaa atgtaagata
     3181 acaaagggga gaaaaagatg tcaatgactt atatggaccc tagggttttt ttgattttag
     3241 aaatggtgtt agaagaagac acaaatactc tagaacaatt ttcaaatgtt ttgggtgtta
     3301 gtactaggac tatcagaaac tatataaacc agattaatcg agaattaaaa ggtggtattg
     3361 ctaaaatagt taaaaatgaa aaaggtacat atagtataag aattgaggat gaaagtaaat
     3421 taattgagat tgtaaatttt aatagaggaa agctatcaaa ttttataaat ttaaattcac
     3481 ctgatgaaag aataaattat gttcttgata ttttaactat gaccaacaat ccaattacca
     3541 tagatgattt ggctgaagag atatgtatag gaagaacaac attaataaag gatttaaaaa
     3601 aagcagacag aattttagct gaatatgatt tagaattaaa aaggaagcca aatataggta
     3661 tgattttagt tggtgaagaa atggatataa gattattaat ccttgataga ttgtatgaaa
     3721 attatattga tgtcttggaa aacgttaatc agataaattt agttaaagat tatgatattg
     3781 aatgtcttag agatgaatta aaaaagttgt ttaaaaaaga agaactctac ataacagaac
     3841 aggtcttgag agatgttgaa agatatataa tcatgtctgt cttaagaaat ctaaatgggt
     3901 acaaatttga aaagatagat aagagatttg aagtgataag atgttcagat gaatatactt
     3961 taggattaaa gctcaaagct ttattggaaa agatatttaa tatagtttta aatgaaaaag
     4021 aaaccatatt tttgacaatg ccattaatag gtagaaaagc tcctagtaca aaattagcac
     4081 ttagcagtat aaagataaat gatagcgtaa aggaagttgt agatgaagta actagttttt
     4141 tattagagac ctcagggatt gattttaaag aagataaaaa gcttatagaa aatttggaat
     4201 atcacttgta ttttgcactt aatagaatga gattcaacat aagggtaaaa aatcctcttt
     4261 tacaagaaat aaaggaaaga tatacatttc catatagtgt tgcaaagata gctgcaatga
     4321 taattgagga aaagtttgac ttgaaaatat gtgaccacga aataggatat atagcattac
     4381 attttggaag ttattttgaa aaaaattcaa gaaaagttct aaatgtaaat agggtagctg
     4441 ttgtttgtgg aacaggtctt gggactgcac aacttattag aataaagata ggaaaaatac
     4501 ttggagaaag tgtagaagta aatacttttt ctgatataga agctaaagct aatttagatt
     4561 tattaaagta ttatgattta gtatttacaa cagtagattt caaaacagac ttagatgttc
     4621 caatttttag agtaaatgcg ttatttaatg aagaaagtat aaagaaagag atagaaaatg
     4681 caatactttt gaaaaatagt ataaatctta agtctataaa aaagattgaa aaaaatatcc
     4741 cttttatagg taaattaatt gaagaggata agttctttat attgaataag agtacgttta
     4801 tggaaaattt agaagatatg ttagaaaaat tagttcataa aggaattatt gatatagagt
     4861 tcaaaaaaaa ggtattaaaa agagaaaaga aatcttcaac agcattagat aattatatag
     4921 cattgcctca ctcagttaat actaatgggg aaaatcttta tctgtcattt ggaatattag
     4981 aaaaaccagt ggtttgggat acaaaggaaa ttagaataat aatattgatg ataattccag
     5041 ataagaatgt agatagtaca gacctgataa taaaaagtta tgaagaagta cttgaagtag
     5101 ggagagataa aaaaatgata gaagaacttg ctaaagtaaa atcatttgat gaattttata
     5161 aaataattac caagaggagg taataatatg gacccagtaa tttttattat agcatcactt
     5221 attacagtgt atttattaaa tatttttctt ggatacttac agcttaaaga ttttaataaa
     5281 aattatattg aactaaaaag aaaaggaaga gtggcaatag gcagaaaaaa aggaagaatt
     5341 tcatcaggta caattgtgtt gatacttata aatgatgatg gtgtgattgt cgaaactaga
     5401 aaaatgcaag gtgttacagt attggcaaga gtaaagtcat ttgaaggttt ggtaggaaag
     5461 agcttagagt acatagaaaa atcagattta actgtttaca ataaactact taaaaaagca
     5521 atactagatg cagtcaaaca gtacataaca tttgagaaaa ataaatgtga agatacaaat
     5581 aatctgagta ctgatgaaga gtgtaggatg gaagtagttt aaaaaaatat atttataagg
     5641 aggagttaat atgacgcaat tttttcaagt attagcaagt ggtgcagata gttttatgaa
     5701 tttatttagg gcaggtgggc aacaatttgt aggttttgta acagatatag taccactttt
     5761 agtagcatta ttagttacaa tgaatgcaat tatcaacttt attggtacag atagagtcga
     5821 gagacttgca aaaaaatgtt ctgcaaatat atttactaga tatttgatat taccagtcct
     5881 tggtacattt gtatttgcaa acccaatgac actatcacta ggaagattct tgccagaaaa
     5941 gtttaaacct agttactttg cagcagcaag cttttcatgc catacaatga atggattatt
     6001 cccacatatc aatccaggtg aactatttat atatctagga attgcaaatg gtataacaac
     6061 tttgggattc tcaacagcag atttagcagt tagatatttg ttagtgggta tagttgcaaa
     6121 ctttattaaa ggtgtagtta ctgactatac aacaaaattt gtagagaaac aacaaggaat
     6181 agcattaaat tcagatataa agattggata attactttaa ggtataaata aatttttcaa
     6241 ataaaaagat agtgtataga ggaggataaa ttatatggca tttaacaaag ttaaaatagt
     6301 aaaaggttct ggaggatggg gtggaccatt gataatagaa ccaacagaaa agaagaataa
     6361 ggtagtatat gtgactgggg gagcaaagcc agaaactgca gtaaaaatag ctgaacttac
     6421 aggatgtgag ttaatagatg gattttcaca gggtgttaaa gatgatgaaa tcgcatgtgt
     6481 aataataaat tgtggtggaa cattgagatg tggtatatat cctcaaaaga aaataccgac
     6541 tataaatata atgaaaacag gaaaaagtgg accattagca atgtttataa aagaagatat
     6601 atatgtttca ggggtaaaac caacaaatgt agagcagtta taattgaggt agtaggaggg
     6661 aagaaatatg gcttatgata ctaaaaagaa gataagtgag cagagtcaag gaattgttgc
     6721 aaaaataggt atagcactag gaaatgtgac aagtactctt tttcaagctg gtagggatac
     6781 tatagataca ttaatacata ctatactgcc ttttatggct ttcgttgcag tcctaatagg
     6841 tataataaat caatcaggta taggaaattg ggtagccaat ttacttactc cattaatcgg
     6901 aaatgttgga ggtttagttg tcttatcaat aatatgttca tttccgttgt tgtctccgtt
     6961 tcttggacca ggagctgtta tagctcagat agtgggggta ttggttggta cagaaatagg
     7021 aaaaggaaat attccaccaa gtttagcatt accagcattg tttgcaataa actcacaagc
     7081 agcttgtgac tttataccag taggtcttgg tcttgcagaa gctgaacctg atacagttca
     7141 agtaggagtt ccgtcagttt tatattctag atttttaaca ggtgcaccaa ctgttctatt
     7201 agcatggtta gcaagctttg ggttatataa atagaagaat actttataaa taattagaga
     7261 gaggttcagt tgatatggaa ttaatataca gtactcaaat aaataaaata ggagataatg
     7321 caaatgaatt tttacaacat aatatgtttg taactttcaa agataatgct cctcaagagt
     7381 tagaaaatta ctgttatata cattctgaaa ataatttagt aaaagaaata cttacgaatg
     7441 atgtgttatc aataaataat gttgattaca aaattacttc tgttggtgag ttagtaaacc
     7501 aaaatctttc agaattaggt catataacat ttaagtttac aggtgaaaaa gatggagcaa
     7561 ttggagggac cttatatctt gaagaaaaag aaatagcacc tattggcgta ggtactatac
     7621 taaaaataat tagaaaataa aatttaaaac gaatctaatc atattataag taaattatag
     7681 ataaaagtca atttaactaa tcaagttaac ttttaatgtg ataaaaaagg ggaaatgaaa
     7741 atgagaaaag tggctttagt tgttggagga ggaagaagtt taggtgaata tttatctaaa
     7801 catttagcta aagtaggtta tgatgttgct attgcagatt taaatgaaga caacgcaaaa
     7861 aaagtagctg aagaaataga aactgaattt ggttgtaaat ctgtaggata tggttgtaat
     7921 gccatagatg aaagacaggt tataagcact gttaaagcta ttgaaaatga ttttgataga
     7981 atagatttat tagtgtacaa tgcaggagtg gctgtaagta aaaaaataac tgattttgga
     8041 tacaaagatt ttaaatttat tgtagacgta aatttaaatg ggtactttct atttgcaaga
     8101 gaagtatcta aagtcatgat agcaaaaggt attgagggta gaatcataca gataaattca
     8161 aaatcaggta gagtgggaag caagcataat tcaggatatt cagcaggtaa atttgctgga
     8221 gtaggactta ctcaaagttt agcgctagat ttggcagaac ataagataac tgtaaatgca
     8281 ctgatgctcg gtaatttact tgattctgat atgtttgaaa gtttaattcc tcaatatgca
     8341 gtaaaattgg gaatacctga atcagaagtc aagcaagttt atatagataa agtaccttta
     8401 aaaagagggt gtagatttat agatgttgca aatatgctta cattttatgc ttcaaaggaa
     8461 gctgaatact gtactggtca atctataaat attactggtg gacaagttat gtaaggtttt
     8521 tatggagtgt ctcaagatga actctttagt tcatgaggca ctttttttgt ataaaattaa
     8581 atatattttc atcatttaaa caatgaaaaa gaggtttatc tttattttga gatagcctca
     8641 tttttgtaat taagaaacat agtgtttaag taaacatata agattgttag ttttttaaaa
     8701 gagaattttt taataatatt atatttatga agtattttta aactt
//
LOCUS       DAMJWP010000149         8718 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.154, whole genome shotgun sequence.
ACCESSION   DAMJWP010000149 DAMJWP010000000
VERSION     DAMJWP010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8718
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.154"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            22..798
                     /locus_tag="PC700_002672"
     CDS             22..798
                     /locus_tag="PC700_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HDF2581969.1"
                     /translation="MEYIIFDLEFNQGFDKKLNKTVSNEKCPFEIIQIGAIKLDSKFN
                     IIDTFNSYVKPTIYKDIHPFISRMTNIKNSDFNDSPTFPEVYNNFIKFISSQDPILCV
                     WGVGDLKELYRNINYHKLPSSSLPKSYINIQQHASKYFNNPAGKSIGLQNAISILELD
                     EKMSYHNALNDAYYTAKVFIKIYNPSIVPDIYLYTSIKPKTIRYSNKKRVDYDKLFDE
                     FRKILNRELTKDEKKIINLAYNMGKTNQFTLENVKQRKNK"
     gene            complement(890..1642)
                     /gene="xth"
                     /locus_tag="PC700_002673"
     CDS             complement(890..1642)
                     /gene="xth"
                     /locus_tag="PC700_002673"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDF2581970.1"
                     /translation="MKFISWNVNGIRACVGKGFLDFFKEVDADIFCLQETKLQEGQIE
                     LDLPGYFQYWNYAERKGYSGTAIFTKKEPLKVMYGINIEEHDKEGRVITLEFEDFYFV
                     TVYTPNSQSELKRLEYRTRWEDDFIDYLTKLDNHKPVIVCGDMNVAHKEIDLKNPKNN
                     MKNAGFTKEEREKFSKLLDSGFIDTYRYFNPDKEGVYSWWSYRFNARKNNAGWRIDYF
                     CASKKLEDRLVSADIHTEILGSDHCPVELEIK"
     gene            complement(1803..2753)
                     /gene="galT"
                     /locus_tag="PC700_002674"
     CDS             complement(1803..2753)
                     /gene="galT"
                     /locus_tag="PC700_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HDF2581971.1"
                     /translation="MKELRIDPITNDVVIFATDRLKRPLDTADIPNEDEETNEYDEEC
                     PFCKGNEAHATDALFEIESEEGWLVKSVYNKFPIIDDMARDVYGVHEVMIESDKHNRS
                     FYNMSQKEFEDVFFMYRNRFRDLSKDDKVEYVSIFKNFLRKAGASLMHPHAQILSMSF
                     IPPEITNELLVSKEYYDSNKSSLYDDLIENEISLDRRVVYNGEAFLVLIPYATKYSGE
                     VRIIFKDKIEFGELNDNNIQELSTIFEKLFKKLYNINGYMPFNLCIHTHPTKIETKSY
                     FNVHMHIIPRKYNFGGFELGTNMYVSSMSPEDLTKKLKID"
     gene            3138..4334
                     /locus_tag="PC700_002675"
     CDS             3138..4334
                     /locus_tag="PC700_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2581972.1"
                     /translation="MNYSNRVSAMQASPIRKLVPFAQAAKDKGIKVYHLNIGQPDIKT
                     PKGFFDAVKNFDSEVLEYATSEGIPELLEALQNYYKTYDMNFEKDELLVTNGGSEALL
                     FTMMAVCDPGDNLLVPEPFYTNYNGFGQSVNVEVNAVTTKAENGFHLPSKEEILSKVD
                     DKTKAIILSNPGNPTGAIYTKEELNILAEIAKEKDLWIIADEVYREFVYDGLEYTSCG
                     NLEGVQDRVIIIDSVSKRYSACGARIGSIACKNKGLIAQILKLCQGRLCVPTLEQIGA
                     VELYKTPVSYFKEVNEEYKKRRDVLYNELMKVEGVICKKPTGAFYIVAKLPVENAEDF
                     TIWMLKEFNKDNETVMVCPAEGFYATPGLGRDEIRLAYILNEKDLHRAATLLKEGLEQ
                     YVALQK"
     gene            complement(4446..5792)
                     /locus_tag="PC700_002676"
     CDS             complement(4446..5792)
                     /locus_tag="PC700_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDF2581973.1"
                     /translation="MTMKKRYNIAIVGGGSTWTPGLLKSLCKMSDRFPLNKVTMLDVV
                     EERQRLIGEFGKILFKEEYPKATLEYTTNPDEAFIDVDFVFVQMRTGGLKMRELDEKI
                     PLQFGLVGQETCGAGGFAYGLRSIGDMIEMVKTVRKYSPKAWILNYTNPAAIVAEALK
                     RVFPDDKRLLNMCDQPVNLLRSYGRLLDMDSRTFEPVYFGLNHFGWFTHLYDKNGEDL
                     VPKIKDLVANSGFQPVDAEQRDKSWLDTYGMVKDMLEDVPDYLPNTYVQYYLYPDKKV
                     AKSNINCTRAREVMNGREKRVFEECKSVIEKGTSIGSNLVHNDAHGEFIVEVAEAIAH
                     NKHQIAIVITENNGAINNLPDDAMVEVAAILTKNGPRPLHVGNIPTFYKGLLEGQLAY
                     EKLAVDAYFEQSYEKAILSLTLNRTIISPTKARQVLDALIKVNKDYWPTLYKENEK"
     gene            complement(5925..6677)
                     /locus_tag="PC700_002677"
     CDS             complement(5925..6677)
                     /locus_tag="PC700_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2581974.1"
                     /translation="MNLIDKLDINQKKLSTNEISLLNYFLKNSEKINQKKIKDIANET
                     FNSTAYIVRFCQKLGFSGYSEFKNFLVFSYSNRNERNKEFTKSQSDIFTDVMDTQNLI
                     NVENIDMCLNEIHNSKHIYFFGVGSSRLVCTEMSQRFSAIGVNVKYYDDSTLMYLAAS
                     NITKDDLVIAVSMSGETAQVIKACNIAKTNQVKVISLTNISVNSLSNIADIKLFVSSP
                     QYNFNSVNYTSRVPALSLMEYMFHKYIEKYNR"
     gene            complement(6836..8413)
                     /locus_tag="PC700_002678"
     CDS             complement(6836..8413)
                     /locus_tag="PC700_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2581975.1"
                     /translation="MKQKMKEFFLQLGKSFLLPIALISAAGIFLGISSAFSNPNIVSD
                     IPFLENNFIQGILGFTKAITGVLFGNLAIFFAISISVGLAKEEKAVSAFSGFVGYLVL
                     HTSVNYMLGCRGILAEPDMMREMGQAVTLGIQTLELGVFGGIVVGIITASLHNKYYNI
                     KLPDYLGFFGGTRFVPIITTVVFSILGIIIPYIWPYVNTIIQSVGIGIASLGYLGTFV
                     FGFLERLLIPFGLHHILNAMFRFTEVGGSLVVGGQEVFGALNIFLAQLSDPETISFST
                     EATRFLAQGKIPIMVFGLPAAAYAMYKTAKPENRSKVKGILLAGALATFVTGITEPLE
                     FSFLFVSPILFVIHAFLSGISFMMMHIFGVAIGNTQGGIIDLVVFGVMQPNTKWIYSI
                     ILGLVYALVYYNVFKVLILRFNLQTPGREEITDINFDLEPLSNKVEIAQSGETTKRAL
                     SIIKYLGGKENIEDITNCITRLRVVVKDMSKVDENAFKKYTGAMGVIKVSDKDIQIIY
                     GPSVSQVRDEVSFAMSI"
BASE COUNT         3070 a         1396 c         1044 g         3208 t
ORIGIN      
        1 aaaatttagg agataaactt tatggaatac ataattttcg atttggaatt taaccaagga
       61 tttgataaaa aactcaataa aactgtatcc aatgaaaagt gtccttttga aattatacaa
      121 ataggagcaa ttaaattgga ttctaaattc aatataatag atacgtttaa tagctatgta
      181 aaacctacaa tttataaaga catacatccc tttattagtc gtatgactaa tattaaaaat
      241 tctgatttta atgattcgcc tactttccct gaggtctaca acaattttat aaaatttata
      301 tcatcacaag accctatatt atgtgtttgg ggagttggag atttaaagga attatacagg
      361 aatatcaact atcacaagct accatcaagt tccttaccta aatcttatat taatatacaa
      421 caacatgcat ctaaatattt caataatcca gcaggaaaaa gcattggcct acaaaatgct
      481 atttctatat tggaacttga tgaaaaaatg tcttatcaca atgctttaaa tgatgcatat
      541 tatactgcta aagtttttat taagatatac aatccaagta tagtcccaga tatatatctt
      601 tatacgagta taaaacctaa aactataaga tattctaata aaaaaagagt tgattatgat
      661 aagttatttg atgaatttag gaaaatttta aatcgtgaat taactaaaga cgagaaaaaa
      721 atcattaact tagcttataa tatggggaaa acaaatcaat ttacattaga aaatgttaag
      781 caaagaaaaa acaaatagag tttatttaga taaaaaatag gcatgtagat taaaaaacta
      841 gttaacttta aatttatatc tacagcccaa tttttatcgt aattaatttt tattttatct
      901 ctaattcaac tggacaatgg tctgaaccta atatttcagt gtgtatatcc gcacttacta
      961 atctatcttc taattttttg gatgcacaaa aataatctat tctccaccct gcattatttt
     1021 ttcttgcatt aaatctatat gaccaccaac tataaacgcc ttctttatca ggattaaaat
     1081 atctatatgt gtctataaac ccactgtcta ataatttaga aaacttctct ctttcttctt
     1141 tagtaaaacc tgcatttttc atattatttt taggattttt taaatctatt tctttatgtg
     1201 caacgttcat atctccacac acaattacag gcttatgatt atctagtttg gttaaataat
     1261 ctataaagtc gtcttcccat ctagttctat attctagtct ctttaattca gattgagaat
     1321 ttggcgtata tactgtaaca aagtaaaaat cttcaaactc tagagttatc actctaccct
     1381 ctttgtcatg ttcttctata tttattccat acattacttt taaaggctct ttttttgtaa
     1441 atatagcagt acctgaataa ccctttctct cagcataatt ccaatattga aaatatcctg
     1501 gtaaatctaa ctctatttgc ccttcttgca atttagtttc ttgtaaacaa aatatatctg
     1561 catctacttc tttgaaaaag tctaaaaatc ctttcccaac acaagctctt attccattaa
     1621 cattccatga tataaatttc atacttatac tcctcatcta aacattgaca cactccccat
     1681 agctaaagat gggggctttt caacaatttt attgtaaatt cttaatttac ttttatttta
     1741 acaatactat actataattt catctataat tactatttac ttaataccta aaaatatatg
     1801 ttttaatcaa tttttaactt ttttgttaaa tcttcagggc tcattgaaga aacatacata
     1861 tttgttccaa gttcaaatcc tccaaaatta tattttcttg ggattatgtg catatgaaca
     1921 ttaaaatacg attttgtttc aatctttgtt ggatgagtgt gaatacataa gttaaatggc
     1981 atatatccat tgatattgta taacttctta aacaactttt caaatattgt agaaagctcc
     2041 tgtatattat tatcatttaa ctcaccaaac tctattttat ctttaaatat tattcttact
     2101 tctccactat acttggttgc atatggtata agtaccaaaa aagcttctcc attataaaca
     2161 actcttctat ctaaacttat ttcattttct attaaatcat catacaaaga gcttttattg
     2221 ctgtcataat actcctttga aactaacagt tcattcgtaa tttcaggtgg aataaaagac
     2281 atagacaata tttgagcatg tggatgcatc aaagatgctc ctgcctttct caagaagttt
     2341 ttgaagatac taacatactc tactttatca tctttcgaca aatctctaaa tctatttcta
     2401 tacataaaaa aaacatcttc aaattctttt tgactcatgt tataaaaact acgattatgt
     2461 ttatcgctct ctatcattac ttcatgaaca ccatatacat ctcttgccat atcatcaatt
     2521 ataggaaatt tattgtagac agattttaca agccatcctt cttcactttc tatttcaaat
     2581 aatgcatcag ttgcatgagc ttcatttcct ttgcaaaatg ggcattcttc atcatattca
     2641 ttagtttctt catcttcatt tgggatatct gctgtatcta aaggtctttt taacctatct
     2701 gtagcaaata taacaacatc attagtaatt ggatctattc ttaattcttt cataattaac
     2761 acctcttagt taatagcctt taatataatt atatgatatt tatcgtatct gttaaataaa
     2821 tttataatat tttttataat tttgcatatt tattattata tattttgtct atagctgaaa
     2881 gattataact aaaaaatcac tatcagtgta tcagaatatt atcttactat aaattaattt
     2941 atgaatacat aatttagtgg cataaacatg ggtattttac tataatttgt taaaagttta
     3001 ttttaaagct atattaattt tttttgatga tttttaccca taagtatgtt attattatct
     3061 tgtaaagatg tcttgtattc aaacatacaa tttacataat acaatatacg tatttaaatt
     3121 aaataggggg tattaaaatg aattattcaa acagagttag tgcaatgcaa gcctctccaa
     3181 tcagaaaact tgttccattt gcacaagcag caaaagataa aggaatcaaa gtatatcact
     3241 taaacatagg acaaccagac ataaaaactc ctaaaggatt ttttgatgct gttaaaaact
     3301 ttgatagcga agtcttagaa tatgctacat cagaaggtat tccagaatta ttagaagcac
     3361 ttcaaaatta ctataaaact tacgatatga attttgaaaa agatgaatta ttagtaacta
     3421 atggtggtag tgaagcttta cttttcacta tgatggcagt ttgtgaccca ggagataatc
     3481 tattagttcc agaaccattc tatacaaact acaatggatt tggtcaatct gtaaatgtag
     3541 aagttaatgc tgttacaaca aaggcagaaa acggtttcca ccttccatct aaagaagaaa
     3601 tattgtctaa agtagatgat aaaactaagg ctataattct ttctaaccca ggaaatccaa
     3661 ctggtgcaat ttatacaaaa gaagaactta atatcctagc tgaaatagct aaagaaaaag
     3721 atttatggat aatagcagac gaagtttata gagaatttgt ttatgatggg cttgagtata
     3781 caagctgtgg taacttagag ggtgtacagg atagagttat aataatagac agtgtttcta
     3841 agagatacag tgcttgtggt gctagaattg gttctatcgc ttgcaaaaat aaaggtctta
     3901 tcgctcaaat acttaaatta tgtcaaggaa gactttgtgt tcctacatta gagcaaatag
     3961 gagctgttga gttatacaaa actcctgttt cttacttcaa agaagttaat gaagagtaca
     4021 aaaaaagaag agatgtatta tacaatgaat taatgaaggt tgaaggtgtt atatgtaaaa
     4081 aaccaactgg tgctttctat atagttgcta aattaccagt tgaaaatgca gaagatttca
     4141 caatttggat gttaaaagaa tttaacaaag ataatgaaac agttatggtt tgccctgctg
     4201 aaggattcta tgctactcca ggtttaggac gtgatgaaat aagattagct tacatcttaa
     4261 atgaaaaaga ccttcataga gctgctacat tattaaaaga aggtttagag caatatgtag
     4321 ctttacaaaa ataatttttt aaacttaatt tatataaatt taatgatata aaatgtcccc
     4381 ttttcagata ttatttgaaa aggggacatt tcttaattaa tatattaatt aagacttaga
     4441 cctgactatt tttcattttc tttatatagt gttggccagt aatctttatt cactttaatt
     4501 aaagcatcta aaacttgtct tgccttagtt ggagagatta tagttctatt taatgttaaa
     4561 gataaaatag ctttttcata agattgttca aagtatgcat caactgctaa cttttcataa
     4621 gctagttgac cttctaacag tcccttataa aatgttggta tattacctac atgtaaaggt
     4681 ctcggtccat tctttgtaag aatcgcagct acttctacca ttgcatcatc aggtaagttg
     4741 ttaatagccc cattattttc tgttataact atggctattt ggtgtttgtt atgagctatt
     4801 gcttctgcaa cttcaactat aaattcccca tgagcatcat tatgcactaa attagaacct
     4861 atagatgttc ctttttctat aacagattta cattcttcaa atacacgctt ttctcttcca
     4921 ttcataactt ctcttgctct agtacaattt atatttgatt tagctacttt tttatcagga
     4981 tatagataat actgtacata tgtattaggt aagtaatctg gtacatcttc caacatatct
     5041 ttaaccattc cataagtgtc taaccaagat ttatctcttt gttctgcatc aacaggttgg
     5101 aatccagagt ttgctactaa atcttttatc ttaggtacca aatcttcacc atttttatca
     5161 tacaaatgtg taaaccatcc aaagtgattt aatccaaagt atacaggctc aaatgttctt
     5221 gaatccatgt ctaacaatct tccataagac cttaacaagt taactggttg gtcacacata
     5281 ttaagtaatc ttttatcatc tggaaatact ctttttaatg cttctgcaac aatagcagca
     5341 ggatttgtat aatttaatat ccatgcttta ggagagtatt ttcttacagt cttaaccatt
     5401 tctatcatat cacctataga ccttaaccca taagcaaatc caccagctcc acaagtttct
     5461 tgaccaacca gtccaaattg tagaggaatc ttttcatcta attctcgcat cttaagacca
     5521 ccagttctca tttgaacaaa tacaaaatct acatctataa atgcttcatc tgggtttgta
     5581 gtatattcaa gtgttgcttt aggatattct tctttaaata atatttttcc aaattcccct
     5641 attaatcttt gacgttcttc tactacatct aacatagtta ctttatttag aggaaatcta
     5701 tcactcattt tacataaaga ctttaaaagt cctggagtcc aagtactgcc tcctccaact
     5761 atagctatat tgtatctttt tttcattgtc atattaatca cccctgtttt ttattaacta
     5821 acttgtaaga tgattataat atataaagtt ttctacatga atacttttag gtaatcatta
     5881 aaaatagttc aattttagta tttttttcaa aattgtaatt tttttcatct attgtatttt
     5941 tcaatgtatt tatgaaacat atattccata agagaaagtg ctggcactct agacgtatag
     6001 ttaacactat taaaattata ttgtggagaa gatacaaata attttatatc agctatattt
     6061 gataatgaat tgacacttat atttgtcaga gaaattactt taacttgatt tgtttttgct
     6121 atattacatg ccttaataac ttgagcagtc tctccagaca tagatacagc aattacaagg
     6181 tcatctttag taatatttga tgcagccaaa tacatgagag ttgaatcatc atagtatttt
     6241 acatttacac ctatagctga aaaacgttga gacatttcag tacatactaa tctagaagac
     6301 ccaacaccaa aaaaatatat atgcttagaa ttatgtattt catttaaaca catatctata
     6361 ttctctacat ttattaaatt ttgtgtatcc ataacatcag taaaaatatc actttgactt
     6421 ttagtaaact ccttatttct ttcatttcta tttgaatatg aaaaaactaa aaagttttta
     6481 aattcactat atccagaaaa gccgagcttc tgacaaaacc ttactatgta tgcagtcgaa
     6541 ttaaaagttt catttgctat atctttaatc tttttttgat ttattttttc agaatttttt
     6601 agaaaatagt ttaaaagaga tatttcattt gtagataatt ttttttgatt tatatctaat
     6661 ttgtctatta ggttcattta tacctccata ataatgattt gtagttaaac aactttctca
     6721 tatttattat ataaatatac actatacaac atttatctta tgtttattat actacaaaaa
     6781 agacaataca aattataaat tagtattgcc ttttaatatt tacattagat agacattaaa
     6841 tagacattgc aaaagaaact tcgtctctta cttgagatac agatggccca taaattattt
     6901 ggatatcttt atcagatact tttataacgc ccattgcacc tgtatatttt ttaaaagcat
     6961 tttcatctac tttactcata tctttaacta ctactcttaa gcgagttata cagtttgtta
     7021 tatcttctat attctcctta ccacctaaat atttaattat ggataatgct ctcttagtag
     7081 tttctccact ttgagctatt tctactttat ttgataaagg ttctaaatca aaatttatat
     7141 ctgttatttc ttctcgtcct ggagtttgta agttaaatct aagtatcaaa accttaaata
     7201 cattataata aaccagtgca tacactaaac caagtattat agaataaatc cactttgtat
     7261 ttggttgcat aactccaaat actactaaat ctattatgcc accttgagta tttcctattg
     7321 ctactccaaa tatatgcatc atcataaatg atatacctga taaaaatgca tgtataacaa
     7381 ataatattgg tgatacaaat aagaatgaaa attccaatgg ttccgtaatt cctgttacaa
     7441 atgttgctaa agcaccagct agtaatatac ctttaacctt cgacctattt tcaggctttg
     7501 cagttttata catggcatat gctgcagcag gaagaccaaa taccattata ggtattttcc
     7561 cttgagctaa aaatctagtt gcttctgttg aaaaactgat tgtttcaggg tcacttagtt
     7621 gagctaaaaa tatgttcaat gctccaaata cttcttgtcc tcctacaact aaagaacctc
     7681 ctacttcagt aaatctaaac atagcattta atatatgatg taatccaaat ggtattaaaa
     7741 gcctttcaag aaatccaaat acaaaggttc ctaaatatcc taaactagct ataccaatac
     7801 ctacactctg aataattgta tttacatatg gccatatata tggtattatt atccccaaaa
     7861 tagagaatac tactgttgtt attattggaa caaacctagt tccaccaaag aaccctaaat
     7921 aatctggcag ttttatatta tagtatttat tatgtaatga tgctgttatt ataccaacta
     7981 caattccacc aaatactcct aactctaatg tttgtatgcc taaagttaca gcttgaccca
     8041 tttctctcat catgtcaggt tcagcaagaa tacctctaca ccctaacata taatttacac
     8101 tagtgtgtaa tactaaataa ccaacaaaac cagaaaatgc agaaactgct ttttcttctt
     8161 tagcaagacc aacagatatt gatatagcaa agaatattgc taagtttcca aaaagtactc
     8221 ctgttatagc ttttgtaaaa cccaatattc cttgaataaa attattttct aaaaaaggta
     8281 tatcactaac tatattagga tttgaaaatg ctgaacttat tcctaaaaat atacctgcag
     8341 cagatattaa tgctattggc aataaaaatg attttcctaa ctgtaaaaaa aactctttca
     8401 ttttttgttt catttctatc cctccatttt taaattttta aaacgttttc tatgtcataa
     8461 ttatatattt ttattttgtt tttgaaactt tataatttgt tttaaaatat ttttacataa
     8521 atagtaattt tttcaaaact gtaaattttt tcaaacaaag acatagtaac attgtgatat
     8581 atagaaattt aaaaagagat tagaaaataa aattattgaa tataaaatgg aaaaatataa
     8641 attacacaaa gaggtatgag taggtactaa caaataagaa aatgtataag aaattataaa
     8701 aaagtacatc aagaaaat
//
LOCUS       DAMJWP010000150         8694 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.155, whole genome shotgun sequence.
ACCESSION   DAMJWP010000150 DAMJWP010000000
VERSION     DAMJWP010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8694
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.155"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            27..851
                     /gene="pdaA"
                     /locus_tag="PC700_002679"
     CDS             27..851
                     /gene="pdaA"
                     /locus_tag="PC700_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HDF2581976.1"
                     /translation="MKKDSLKKYIMIGAFALILFGIASINFKSLDKTKTQISSPTLDT
                     HEYDWYFNPREDGKQPSPIKEADFFKKYGAYYVGNPNEKVIYLSFDAGYESGNTPKLL
                     DTLKKHNAKAQFFVVESYIKSNPELIKRMEKEGHLVCNHSKSHPSMAGITDFEKFKEE
                     ITSVEKAYKDVTGKEMPKYFRPPMGKFSEQSLKYTQDLGYKSIFWSFAYVDWYEKKQP
                     THEFAKNKIYSRTHPGAIVLLHPNSSTNTEILDEVLTHWEKEGYKLKTLDYLNNKK"
     gene            1040..1804
                     /locus_tag="PC700_002680"
     CDS             1040..1804
                     /locus_tag="PC700_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2581977.1"
                     /translation="MTTICVMIDHIFLDTEYRQPTIHKHLAKHIIISQSGNLSVTFDK
                     GESLQCKGIIIDSNVSHTVSSKDNKMLVFLIDDTSILAQNISQHQLLNHSFFVIDNLI
                     VERIQNIYTACQVKGLSQSYIDFSKQAFRLLNLNYHISSITDSRIQWALNHILKSTHI
                     HNEIFFEICAEVKLSKSRFSHLFKEQMGVSLNSYLALSKLRKAYELLLKSGDITSAAM
                     DAGFSSPSHFSASSKKYLGITASELIGKCKIYFIRQ"
     gene            1907..3085
                     /locus_tag="PC700_002681"
     CDS             1907..3085
                     /locus_tag="PC700_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDF2581978.1"
                     /translation="MKTNKISFNKGLYYLNTNANFNFQLNRIIMWDGGRLEDIQKVSS
                     KIVDSESWKTELMYLAQEAEQDGRTENAIAYYRMSEFFMYDGDPDKISVYKKATTLFY
                     NYYSEYFSNGTVQIFHVPYENITLPVMYAKAQGEVKDVILLHGGNDSYFEEFFFPMLY
                     LASQGFDIYLFEGPGQGGVMRLQGKHFTYQWERPVKTILDYFHLENITIVGASLGGFL
                     APRAAAFEKRIERVVAWSVFPNFLDIIVGSQPIKMQNLLRLLLKLKTKWLVNFIIKQK
                     MKSGDSMVVWGLKHGMYAYEASTPYEYFQKIKYYQIDSIAKNINQDILILGATQDHFI
                     DYRTVGKEIDLLNNVRSLTFRLMTEAEQASNHCNCGNSKLVLDTISNWIMMMKIHDRV
                     "
     gene            complement(3456..4241)
                     /locus_tag="PC700_002682"
     CDS             complement(3456..4241)
                     /locus_tag="PC700_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2581979.1"
                     /translation="MIVKIDNTLEKEFWQYVSHEESLNLFIIGYVENYGFSSQYQDIW
                     SQVEDGNITSIILKNKSTLIIYSFKNNFDIGEMKNHIKDLDVESISGKKCVIDRLISK
                     YKDFYEKLDNKFCVLKEIKEIDFSNMKEYKIENAQEKDIDEIGKLLNRSDYKVSKNYI
                     EERKEHLKEGNVRAYFIRNDDTMISTVSTGMETSFLAMVVSVSTDKRYRGKGLASYMV
                     YNLSKELLLEGKVPCLFYNNDVAGKIYHNIGYKEINEWTILFK"
     gene            4706..5071
                     /locus_tag="PC700_002683"
     CDS             4706..5071
                     /locus_tag="PC700_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2581980.1"
                     /translation="MKIRAEYTCPLEIVHDIIKGKWKTILIFELRQSNKTFSELQHNI
                     YGINQKMLIQQLKELRDFGIIDKISSAGYPLHVEYFLTNRGKKMLKAVEIMQEIGIEY
                     MLEHGQQEILDNKGICYNK"
     gene            5149..5703
                     /locus_tag="PC700_002684"
     CDS             5149..5703
                     /locus_tag="PC700_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDF2581981.1"
                     /translation="MSIALILIDIQNDYFKNGKCELFQSEETAENAKKILLFFRKNNL
                     PVIHIQHISTDKTASFFLPNTHGSEINKIVFPLDNEEIIIKHTPDSFFETDLQSILEK
                     KNITNLVICGMMSHMCIDTSVRTAKKLRYDITLISDACTTKNLSWNNIEINANTVHQV
                     FMASLQNSFADVKNTDKFLSNYHK"
     gene            5923..6597
                     /locus_tag="PC700_002685"
     CDS             5923..6597
                     /locus_tag="PC700_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDF2581982.1"
                     /translation="MNHHILIQFEYIARIITAGFCGALIGYERKNRLKEAGIRTHFIV
                     ALASALIIIVSKYGFNDIVGTPGIGLDPARVAAQVVSGIGFLGAGLIFIRNQSVSGLT
                     TAAGIWATAGTGLAIGSGLYLVGIVSSIIIVIVQITLHRDRKWMKLPTAEQVSLKISN
                     STDSIEYIEGKFKKSNIEIINMNIKRLDGDWLEVVIYTKLPKGYKKTNLLKLFSDNPL
                     IETLEI"
     regulatory      6952..7048
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            7102..7278
                     /locus_tag="PC700_002686"
     CDS             7102..7278
                     /locus_tag="PC700_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581983.1"
                     /translation="MFRDEMDKCTHMLTAYISSLYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            complement(7466..7705)
                     /locus_tag="PC700_002687"
     CDS             complement(7466..7705)
                     /locus_tag="PC700_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581984.1"
                     /translation="MKCSNCGSVNIEKGILTTGGIYAETIGLKYRGDGKLDKVINKTF
                     PVQSTIYSDLCLDCGEIVRTYIKDNTDRNWCKDKK"
     gene            complement(7850..8659)
                     /locus_tag="PC700_002688"
     CDS             complement(7850..8659)
                     /locus_tag="PC700_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2581985.1"
                     /translation="MKDYYKIGEISKIYGIGRDSLMYYEEIGILRPVRDINGYRMYNI
                     SDIWKLNLIKEFRSLNFPMKKIKEYLDDRSIESTKNILNEELDLIDKKIAEFISHKEN
                     IIKRLSSIESVIQNTKIDEIEVVYIEKRKALELNADIKRDEDFDFLIQKLQKEYEDRF
                     NILGNNNIGSAFSTEAINKGIFNEFKSVFCFLESNEKVYNIVFDEGYYVTLNYTGSNS
                     NNKMYMEKVFKFIEENNYKIIADPIEIYKIDIHETGIVEEFVTEIQVPITK"
BASE COUNT         3127 a         1288 c         1126 g         3153 t
ORIGIN      
        1 ttaaatttta aataagaggt gaaattgtga aaaaagatag cctaaaaaaa tacattatga
       61 taggagcatt tgctctaata ctttttggaa ttgctagtat aaattttaaa tcattagata
      121 aaacaaaaac tcaaatttct agtccaacac tcgacacaca tgaatatgat tggtatttta
      181 accctaggga ggatggtaaa cagccatcac ctataaaaga agctgatttt tttaaaaaat
      241 acggagctta ttatgtagga aatcccaatg aaaaagttat atatctatca tttgatgctg
      301 gatatgaaag tggaaacact ccaaaattat tagatacgct taaaaaacac aatgctaaag
      361 ctcaattttt cgtagttgaa agttatataa aaagcaatcc agagttaata aagcgtatgg
      421 aaaaagaagg acatttagtt tgtaatcatt caaaaagcca tccatcaatg gcaggtatta
      481 cagattttga aaaattcaaa gaagaaatta caagtgtaga aaaagcatac aaagatgtta
      541 ctggaaaaga aatgcctaag tattttagac ctccaatggg taaatttagc gaacaatctt
      601 taaaatatac acaagattta ggatataaat caatattttg gagttttgct tatgtagatt
      661 ggtatgaaaa gaaacaacct actcatgaat ttgccaaaaa caaaatttat tcaagaactc
      721 atcctggtgc aatagtttta ctgcatccaa attcatcaac aaatactgag atattagatg
      781 aggttcttac acattgggaa aaagaaggtt ataaacttaa aacacttgat tatttaaata
      841 ataaaaaata gtatcataaa ttttaaaaaa ctctatcatg aatcatagta aaaatatagt
      901 tatttataaa tactttaatt ctcgatagag ttttttattt ttcattgcct aaaataggat
      961 ttttaaatac ttgtaatcac taagctttct tgttaaaaac tgtataatat gctaagctat
     1021 gaacaaggag ttgacagcta tgactaccat ttgtgtaatg attgaccata tatttttaga
     1081 tacagagtat agacaaccaa ctatccataa acatcttgca aaacatatta ttatatcaca
     1141 gtctggtaac ttatctgtga cgtttgataa gggcgaatca ttacaatgta aaggaattat
     1201 tattgattcc aatgtatcgc atacggtgtc aagtaaagat aataaaatgt tggttttctt
     1261 gatagatgat acaagcatac tcgcccaaaa tattagtcaa caccaattac taaatcactc
     1321 ttttttcgtt atagataatc ttatagttga acgaatacaa aatatttata cagcttgtca
     1381 ggtaaaagga ttgtcccaaa gctatattga tttttcaaaa caagctttcc gtctattgaa
     1441 tttaaattat catatttcgt ctattacaga cagtagaata caatgggcat tgaatcatat
     1501 tctaaagtcc acacacattc acaatgaaat cttttttgag atatgtgccg aagtaaaatt
     1561 gtccaaaagc cggttttctc atcttttcaa agaacaaatg ggagtatcac taaacagcta
     1621 tttggcttta tcaaaacttc gcaaggctta tgagttattg ttaaaaagtg gagatattac
     1681 ctcagctgct atggacgcag ggtttagttc accgtctcat ttctcagctt ccagcaaaaa
     1741 atatcttggc attactgcaa gtgagcttat aggaaaatgt aaaatatatt ttatccgcca
     1801 atagcgtcta tttgaaagtc agtacttcta atatttgtta caataaatgc atagcattaa
     1861 agattactct ttattgctat gcattatctt tttaggagtg atgacattga aaacaaataa
     1921 aatttctttt aataaaggtc tatattacct taatacaaat gccaatttta attttcagtt
     1981 aaacaggatt attatgtggg atggggggcg cttggaagat atacaaaaag tttcctctaa
     2041 aattgtcgat agcgaaagtt ggaaaacaga acttatgtat ctggcacaag aagctgaaca
     2101 agatggacgc acagaaaatg caattgctta ctatcgtatg agtgaatttt ttatgtatga
     2161 tggtgaccct gataaaatat cagtctacaa aaaagcaact actctattct acaactacta
     2221 ctctgaatat ttctcaaatg gaactgtaca aatttttcat gtaccatatg aaaatatcac
     2281 cctacctgta atgtatgcaa aagcgcaagg cgaggtaaaa gatgttatat tgctccacgg
     2341 tggaaatgat tcttactttg aagaattctt tttcccaatg ctttatcttg cgtcacaggg
     2401 atttgatatt tatctttttg agggacctgg tcaaggtgga gtgatgcgtt tgcagggaaa
     2461 acactttact tatcaatggg aacgtcccgt taagacaatt ttagattatt ttcatttaga
     2521 gaatataact attgtaggtg cttccttggg aggttttctt gctccacgag cagcagcctt
     2581 cgaaaaacga attgaacgcg tggtagcttg gtctgtattc cccaatttct tagacataat
     2641 agttggttca caaccaataa aaatgcaaaa tctacttaga ctactgctaa aactcaaaac
     2701 aaaatggcta gtcaatttta taataaagca aaaaatgaaa agtggcgata gtatggtggt
     2761 atggggttta aaacatggaa tgtacgcata tgaagcgtct acaccctatg aatattttca
     2821 gaaaatcaag tattatcaaa tagattctat tgcaaaaaat ataaaccaag acattttgat
     2881 tttaggtgca acacaagatc attttattga ttatagaact gttggtaaag agattgattt
     2941 actcaacaat gtccgatccc ttacatttag gcttatgaca gaagcagaac aagctagtaa
     3001 ccactgtaac tgtggaaata gcaaactggt tttagataca atttcaaatt ggattatgat
     3061 gatgaaaata catgatagag tctaaatatt gatttttaaa ttatacaaaa tccatctgct
     3121 agcatatcct tattgctttg ttcaaataat cacttgttaa aaaattggtt cccacttacg
     3181 aaatactacg tttatagaaa tgaactatat catgtaagca cacaatatat atgataataa
     3241 gacccaatta ctcaatgtaa ctgggtcttt cttatgtcat agattttata ggactcatga
     3301 aatgtaaaag agtatttcac cgatttccaa agggaacttt acttagataa ggagaccata
     3361 aatgctaaca aacccatcta ctagacacct ttcaagtatt tgagcgatta tacaatacac
     3421 gttaagaatt acacgaaaat ttattatttt tatagctatt taaaaagaat tgtccattca
     3481 tttatctctt tgtatcctat attatgatat attttacctg ctacatcatt attataaaaa
     3541 agacatggaa cttttccttc caacaataat tctttactca aattgtaaac catataactt
     3601 gcaagtcctt ttcccctata tcttttatct gtacttacag agaccaccat tgccaaaaaa
     3661 ctagtttcca taccagttga cacagtgcta atcatagtat catcatttct aataaaatat
     3721 gctcttacat ttccctcttt taaatgttct ttcctttctt ctatgtaatt tttacttact
     3781 ttatagtctg acctatttaa aagttttcct atttcatcaa tatctttttc ctgagcattt
     3841 tctattttat actccttcat gttggaaaaa tctatttctt ttatttcttt taatacacaa
     3901 aatttattat ctagtttttc ataaaaatct ttgtatttac ttattagtct atctattaca
     3961 catttttttc cacttatgct ttctacatct aaatctttta tatgattctt catttctcct
     4021 atatcaaaat tatttttaaa actataaatt ataagtgttg acttattttt taatattata
     4081 gaggttatat ttccatcttc tacctgagac catatatctt gatattgaga gctaaatcca
     4141 taattctcaa cgtatccaat tatgaataaa tttaaacttt cttcatgtga tacatattgc
     4201 caaaattctt tttctaaagt attatctatt tttacaatca agcagatatc ctccttctat
     4261 gttaataatg atatacctta taatttatag tatcatttaa aatcattata ttctaccaaa
     4321 tattctaaat ttttaaatat ctatgcaata tgttcgtatt tatttttata ttctgttact
     4381 gcttcactcc aagcttcttc tactgaaata cctctcttat ttgcaatttt ttgtgctaat
     4441 tctattattt tcatagcttt tcttgatata ttcatatata ttcctcctct aaattatcat
     4501 aagttatttt aaagttaaat ttctatagtc atgttatata tttatttact tatatcttac
     4561 taattattta tgtactaaat atgaactaca atagaagaaa tttagaactt aataaaacat
     4621 aaaatttata tacagttcat atatatttgt catatcacaa gacaagtgat ataatttatt
     4681 atcattttac tgggggtgat atgctatgaa aataagagct gaatatacat gtcctcttga
     4741 aattgtacat gacataatca aaggaaaatg gaaaactatc cttatatttg aattaagaca
     4801 atctaataaa acattttctg aattgcaaca taatatttat ggaataaatc aaaaaatgtt
     4861 gattcaacaa ttaaaagagt tacgtgattt tgggattatt gataaaataa gttctgctgg
     4921 atatccatta catgttgaat attttcttac taatcgtggt aaaaaaatgt taaaagcagt
     4981 ggaaataatg caagaaatag gaatcgaata tatgcttgaa catggtcaac aagaaattct
     5041 agataataaa ggaatttgct acaataagta atcctataca taccaaaaag taagtaattt
     5101 acatttttta caatagtatt ataataaaag taaatagaag gtataatcat gtctatagct
     5161 ttaatattga ttgatattca aaatgattat tttaaaaatg gaaaatgtga attatttcaa
     5221 tcagaagaaa ctgctgaaaa tgctaaaaaa atactactgt tttttagaaa aaacaattta
     5281 ccagttattc atatacagca tatatcaaca gataaaacag cttctttttt tcttcctaat
     5341 actcatggtt cagaaataaa taaaattgtt tttccactag ataatgaaga aattataatt
     5401 aaacacacac cagatagttt ctttgaaaca gatttgcaaa gtatccttga gaaaaaaaat
     5461 attactaatc tagtaatctg tggaatgatg agccatatgt gtattgatac aagtgtaaga
     5521 acagcaaaga aacttcgata cgacataact ctaataagcg atgcatgtac aacgaagaat
     5581 ttatcttgga ataatataga aattaatgca aatactgtac atcaagtatt catggcatca
     5641 ctacagaaca gttttgcaga tgtaaaaaac acagataaat ttttatctaa ctatcacaaa
     5701 taaaaacata cactatgtta aaagagcaag tttgatttat ttaagcttgc tctttaatca
     5761 tttaaaatta gatgctttct atcattttta tatacataat atacgtttat acattttata
     5821 ttcatatata actattatat aaattttaaa ctttactata ttcacataaa aaatgtagta
     5881 ttatagtata tataatatta tatagaaggg ggtgctagat aaatgaacca tcatatattg
     5941 attcaatttg aatatatagc aagaatcatt actgctggtt tctgtggggc tttaattgga
     6001 tatgagagaa agaatagact aaaagaagct ggaatacgca ctcattttat agtggcatta
     6061 gcttcagcac ttataatcat tgtttccaag tatggattta atgatatagt gggtactcca
     6121 ggtattggtc tagacccagc ccgcgttgca gcacaagttg tcagtggtat aggtttttta
     6181 ggtgctggat taatatttat tagaaatcaa tcagtaagtg gtcttactac agctgctggt
     6241 atatgggcaa cagccggtac aggtcttgcc attggctctg gattatactt agttggtatt
     6301 gtttcaagta tcattattgt tattgtacag ataacacttc atagagatag gaagtggatg
     6361 aaactcccta cagcagagca agtttctctt aaaatttcta acagcactga ttccatagaa
     6421 tatattgaag gtaaatttaa aaaatctaat attgaaatta taaacatgaa tattaaacgc
     6481 cttgatgggg attggttaga agtagttata tatactaagt tacctaaagg atataaaaaa
     6541 acaaatttat taaaattatt tagcgataat cctcttatag aaactttaga aatataatat
     6601 aaaaattgaa gcttaaaaat aataatatat ttaatcaagg tcaatatata aataattacg
     6661 ggatgagtct atataaaatt catcccttgt tttttgccat tcatatatat tttttcaaga
     6721 tatttttgct catctattat ctagccaagt aactcttgtg aactaaactt tttaatacaa
     6781 aaacttcaat acaaaactaa atataatttt tattaaatat tctacatgta tagtttgttt
     6841 cttgagtgaa aaatagattt catattgtta agatactgtt aaatatttca attttctgac
     6901 aagtatgttg aacaataact tatcctagtg tattatttta gttgtagaat aaaactaaat
     6961 cggcaaaact agagaaattt agtgacgcaa agctataggg actaacgctt ataaaataaa
     7021 ctatgagtta tgtcagccag ttgccaaaaa gatattgttc tttttgtttt atgaaagttt
     7081 tttaggggga tagtaatata tatgtttaga gatgaaatgg ataaatgtac acacatgtta
     7141 actgcttata ttagtagttt atatgattat tgtgatttta tagatacaca gctagatgat
     7201 tttatactag agtacggaga aaatgtagta gaatcttgtt tacatcaagt gatggtattg
     7261 gtaagtaagt ataattaaat agaaattgtt ttctagagaa ccctttttag ttttatctac
     7321 atttaaaaat gttattatat taaaaaacta atctccccta tatgcaaagt tttcttccat
     7381 ataacaattt cttcccattt atactttttc acttgtatct ttaataaaat tcaacttcaa
     7441 atattaatat acttcaaaaa ttattctatt tcttatcttt acaccaattc ctatcagtat
     7501 tatcctttat ataggttcta actatttcgc cacaatctaa acataaatct gaatatatag
     7561 tagattgtac tggaaaagtt ttatttataa ctttatctaa ttttccatct cctctatatt
     7621 ttaatccaat agtttcagca tatatgccac cagttgttaa aatacccttt tcaatattta
     7681 cacttccaca attagaacat ttcacattta taacccccta aacaattatt ttttcatgct
     7741 atcttataat ttaaaatata atttgctacc gattttataa cacagtagca aatcggtggc
     7801 aaatcaacat tttttcattc tatttaccat ctaatccatt gaaatatggc tatttagtta
     7861 taggaacttg aatttccgtt acaaactctt ctacaattcc agtctcatgt atatctatct
     7921 tatagatttc tatagggtca gcaattatct tataattatt ttcttctata aatttaaaca
     7981 ccttctccat atacatctta ttattagaat tactcccagt ataatttaaa gttacataat
     8041 atccttcatc aaaaactata ttatatactt tctcattact ttccagaaaa caaaatacag
     8101 atttaaactc attaaatata cctttattaa ttgcttcagt agaaaaggca gaaccaatat
     8161 tgttgtttcc aagtatatta aatctatctt catattcttt ctgaagtttc tgaattaaaa
     8221 aatcgaagtc ttcatccctc tttatatcag catttaattc taaagctttt cttttttcta
     8281 tatatacaac ctctatctca tctatttttg tattttgaat tacactttca atcgaactca
     8341 atctttttat aatattttcc ttatggctta taaattcagc tattttttta tcaattaaat
     8401 cgagttcttc atttaaaata tttttcgtag actctatact tctgtcatct aggtattctt
     8461 ttattttctt cattggaaag ttcaagcttc taaattcttt tattaaattt aatttccata
     8521 tatctgatat attatacatt ctatatccat ttatatctct aactggtctt agaatgccta
     8581 tttcttcata atacattaat gaatctctac ctattccata tattttagat atctctccta
     8641 ttttatagta atctttcatt tatataataa actcctccga aatcctattt ttat
//
LOCUS       DAMJWP010000151         8671 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.156, whole genome shotgun sequence.
ACCESSION   DAMJWP010000151 DAMJWP010000000
VERSION     DAMJWP010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8671
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.156"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(4..1776)
                     /locus_tag="PC700_002689"
     CDS             complement(4..1776)
                     /locus_tag="PC700_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HDF2581986.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDIILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYNMDKHPALDSMTRRIVNDIRNIDTN
                     QDMMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(1790..2719)
                     /locus_tag="PC700_002690"
     CDS             complement(1790..2719)
                     /locus_tag="PC700_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2581987.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            2948..3136
                     /locus_tag="PC700_002691"
     CDS             2948..3136
                     /locus_tag="PC700_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2581988.1"
                     /translation="MEYMSCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKN
                     AEKPVDGRAKRRKEVQYE"
     gene            3129..3830
                     /locus_tag="PC700_002692"
     CDS             3129..3830
                     /locus_tag="PC700_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2581989.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHCGAEGLHLLDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDCIIINGMIIDKTNRIVSINNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            3832..4755
                     /locus_tag="PC700_002693"
     CDS             3832..4755
                     /locus_tag="PC700_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2581990.1"
                     /translation="MNTSVYLLLILALSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            4912..5835
                     /locus_tag="PC700_002694"
     CDS             4912..5835
                     /locus_tag="PC700_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2581991.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHVKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGCEKRVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSKYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDHTLRLYNTTLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            5828..6565
                     /locus_tag="PC700_002695"
     CDS             5828..6565
                     /locus_tag="PC700_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2581992.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPTKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFASIALGRRK"
     gene            6576..7349
                     /locus_tag="PC700_002696"
     CDS             6576..7349
                     /locus_tag="PC700_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2581993.1"
                     /translation="MWKLLWAECQKIRRSKIVWITFFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETKLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIISLLLCACLSAFILKGLKHNEKN"
     gene            7365..7517
                     /locus_tag="PC700_002697"
     CDS             7365..7517
                     /locus_tag="PC700_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581994.1"
                     /translation="MKKIKKLIILIVFLFLFGMLHCYTEWKFNNDPPKGVIERINEWT
                     DKIGLD"
     gene            complement(7890..8498)
                     /locus_tag="PC700_002698"
     CDS             complement(7890..8498)
                     /locus_tag="PC700_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDF2581995.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
BASE COUNT         2869 a         1441 c         1247 g         3114 t
ORIGIN      
        1 ttattaattt ctaattatct tctttaaata cattgaaact acatcagaaa atctagttat
       61 atctcttgaa tatataaaat catttccata tattaatctt tcagcttcaa gttctgattc
      121 ttttcctgta aatactccta gtactaatat attttgcttt ctagcttttc tcacttcatt
      181 ggctgtatct ctcaaaccaa ctatacccct ataagccttt tctcctctta atgttctctc
      241 actacttttt cctatcttta catcatttgg tcttccatcg ctgagaacta tcaggatttt
      301 attttcttca tctctattta ttagaccact acatatagat tttattgcca aaccatctct
      361 attattacca gcacaaaaat attcaaatat attttcacta ttttttatac tatcatcata
      421 atctttaaat ctttttaata ttgtatagtc taaaaaacta gaaaatccca tcactctatt
      481 tggtatgcca acttctgtta gagcatttgc aattatgtaa gcttgaattg ccacatttcc
      541 ctgatttcta ctttgagagc cacttgcatc caataatata tcaataacgt atttcccttt
      601 ttcattttct atatctttat aaaatatttt attattatta cttctaccaa ctctccaagc
      661 tctatttgct aatatagtac cacaatcaga atatactcta cttttagcat tttcttctat
      721 taaaattctt gacagacttt cttttaatct ctttatttgc tgtctataca ttctagcatg
      781 ttctctaaat tttcctaaat tattttcttt ttgtcttgtt acatatttta gttgaaatac
      841 attattacac tcacttctaa gaactccatc tgtaaagtgt attctacaac cttcatgaac
      901 atttctacaa ttttgtgtct caattttttt aatttcacct ttactcaaat aagtttttcc
      961 ataataatgt tctatttttg aatatatctt atttgcagtg tcttcatcaa catatataac
     1021 tcggtctgct gaattgctac atttagaatc atcaaaatcc cctacacctt ctacaagcat
     1081 ggatgaagat attgaatcta tatcactttc aataatattt tcttcttcat cactataaag
     1141 ttcttcatac atgaaatcag caaatgtatc aaaatttact tctacacttt ctacattatt
     1201 ttcatcaata agactatttt cttctccatt taatatgtat tcgaaataag ttaaatatat
     1261 aacatctaat gcttttaaaa tctccatcat atcttgattg gtatctatat ttcttatatc
     1321 attaactatt cttctagtca tactatctaa tgctgggtgc ttgtccatat tataaagtac
     1381 aaatgtgtat ttaattttat ccataatatt atcattgtgt attttggtaa aacgtgaaac
     1441 aatatcttta taagcatttc ttcttatgtc ttctatccct ggcctatctt taatgacctt
     1501 ttcttcaact attgtattta atataacttg aattagaccc agtataatat ctttgttgta
     1561 tccttttctt attctatttg taagatattt tttaactata tcccaatcta catactttct
     1621 tctagcacct gcaattattg caaaatatag acctatttct tttgaagagt aatctttttc
     1681 tgatatatct atactttcat tgtaatcacc agctattgtc catgataagt tattagctct
     1741 attttcaaat tcataatcat catatatcca tttcataatc tcacctatat taaatatgac
     1801 ttattggaaa tacatctata ctctcccatt tattaggtat tctagtttta attacatctc
     1861 caaccatttc tttctcatat acatcaaaag tctttcctgt aagtcccata ttaattgcaa
     1921 gagttggttt taatcctctt cttatagtct taagagatgc cataagacca cgtaaatcaa
     1981 tagctttact agatatctct ccattttgag acttcaattg taaatctaaa aatattcctg
     2041 caaagtgtat taatttttct tcatcagcat ctgaaaattc attttttaaa atcatcatta
     2101 acttatcttc ttctatagga ggaatatcta ttaccataaa tctagatacc agtgcttcat
     2161 taagttcttt tgttcctgca tattcataat tcatagtccc aataaatctt gttgctggat
     2221 gtagattgac tctctcatac ccaggtacat caattattct tctatagtct aaagctgaat
     2281 gtaccacaac tatagcatca ttttttgcca tattaatttc atcaaatact ccaaaacctc
     2341 cattaatagc acactcataa actgagcctc ttctaagttt aacttcattg tctataaatg
     2401 tatctgtacc tataagtgtt gaactgtctg tatttatatg aaatgatgta ttccactgag
     2461 gtctattaaa tatttcccct agattatcag caagtaaatt ttttcccgtt gcctttggtc
     2521 ccgataataa tatattttct tcttctaaaa tagctgctat acacatagac aagatatctt
     2581 taccataaaa ataagtttta gactttgtta ctctgtattc taaatctttt tctaaaccat
     2641 aataatttct aaaatacagt acatcttcaa taagtttttc atctacccct tgcaccctta
     2701 gattatctat tatatccata tttttacccc ttttatataa atagtatttg tttcatttct
     2761 tatcaccatt ttagcataaa aggatataag ctaaaattga ttttttaatc tcccccattt
     2821 taagtaaaac catgcaccat tttatcacta caaaattaag agtactactt actgatattt
     2881 cttgattttg ttcgtctata cgtatataat atatgctgat aaattatgag gaaaggatga
     2941 ttgaaatatg gaatatatgt cttgcccaga agtagcaaaa aaatggggca tttctgaaag
     3001 aagagtacaa aaactatgtg aagaaagtcg cataccagga gtttcaaaac tcgggtatat
     3061 gtggctgata ccaaagaacg cagaaaagcc tgttgatggt agagcgaaaa gaagaaagga
     3121 agtacaatat gaatagcgtt ttgattatag atgatgacaa agaactttgt gctcttatga
     3181 aaaaatgtgt tgagcaagaa aatttatctg ctattactgc acattgtggt gcagaaggtc
     3241 tacatttgct cgataaaaat agacacacac tctctcttgt aattttagac ataatgatgc
     3301 cagatataga cggttttcaa gttctgaaaa aaattagaga aacaagtaat attccagtaa
     3361 ttatgcttac tgctaaaaat gacgaggatg ataaagtttc cggactacgt ttaggtgctg
     3421 acgattacct aacaaaaccg ttcagcataa acgaacttat ggctcgtgtc aattctctta
     3481 ttcgtcggta tactatgtta aaccctgctt ttataacaga cacagattgc ataataataa
     3541 atggcatgat tattgacaag acaaaccgaa ttgtttctat caataatatt cctgttcaac
     3601 ttacaagcaa agaatttgat ttacttgctt ttctggcttc taataaggga cgggtattta
     3661 ccaaaaaaca gatttataca caagtatggg aagaagaata tgcttttgac gatagtaaca
     3721 tcatgtcttt catcagtaaa ttgagaaaaa agattgaacc agaccccaac catccatttt
     3781 atattttgac agttcgtggt gtcggttatc gttttaataa ggaggcttaa catgaataca
     3841 agtgtctatc tacttttgat attggctctt agcctgatta ttatagcgta ccttgtttct
     3901 gtcctgctac gtgtccgaat acaacttaca cttataaaag atgctttaga ggatataaaa
     3961 aatggaaacc ttaatcgccg tattttaaca gatgaaaacg acattacaaa acaaatttgc
     4021 tatgatatta acgaaatagc aatcaatagc caatttcaac ttattcagca aaaacaatca
     4081 gaacaagcct acaaacagct aatgacaagt ctctcccatg atgtaaaaac tccacttgct
     4141 tctttggtcg gttatttgga agcagtagaa agtaaaattg ttgtaggaga ggaaaaagac
     4201 gagtatatcc atgttgcgtc taataaggct cattacttaa agaattttgt agaaaatcta
     4261 tttgaatggg taaaattgga ttctaaggaa caagtttttc attttgatat ttttgacttg
     4321 aatgaattat cccgtaatat tatatctgat tggatacctg ttttagaaag cagtcatttt
     4381 gaatatgaat ttgatatacc agaaatagag tacttcttac gtatagacgc aaatgcatat
     4441 acccgtatca ttaataacct attacaaaat attattaccc atagtagtgg caacaaaatg
     4501 acattgagaa tttttgaaaa caaagagcaa gctcaaatta taataacaga taatgggaaa
     4561 ggtatatctt cagacaatct cccacatatt tttgaaaggt tgtaccaatg cgaccattca
     4621 cgagcttcaa agggaaatgg tttaggcttg gcgattgcaa aggaacttat taacgctcat
     4681 aagggaaata ttaccgccaa cagcactccc ggtatgggaa cagaatttac aatattgctt
     4741 cctaaagccc tgtaaaagta cgaggctttc ttactctaaa acaagataaa agcaagatta
     4801 tggcaaggtt ttggcaaggt tcatgtttta taattctaat tgtagttgat tttgacgttg
     4861 tatcaactat cttttgtaga aacaaattta ttaaaagtga ggaaattata tatgaatgat
     4921 tttgtgattg aaacaaagca acttacaaaa atttatggga atcaaacagt agttgacaaa
     4981 gtcaatctcc atgtaaaaaa aggccgaata tatggtctgt taggacgcaa cggagctggg
     5041 aaaaccacaa ttatgaaaat gattttagga ttgacttcaa tcacgtatgg cgaggtagat
     5101 gtatttggag agaatattaa gggatgtgaa aaacgagtat atccccgtat cggtgcaatt
     5161 atcgaaacgc caggatttta tcctaatctg acaggaacgg agaacttgga gatttttgca
     5221 aaacttcgtg gtacagccgc ccctaattct gtaaaaagag cattggagat tgtaggctta
     5281 ccttataaaa ataaaaagct gtttagtaaa tattcacttg gcatgaaaca gcgacttggt
     5341 attgccaacg ctatcctgca tgacccggaa ctgttagttt tagatgagcc aaccaatgga
     5401 cttgacccta ttggtattgc ggagatgaga aagtttatta gggacttgag cgttgagcat
     5461 ggaaagacaa ttcttatctc cagtcatatt ctttcagaaa tctcattgtt ggctgatgat
     5521 attggaatta tcgaccacgg tgttcttttg gaagaaagta gtatgagtga actagaaaag
     5581 aaaaacagta aatatatcct gctacaagtt tcagatattc caaagacatc acttatttta
     5641 gagcgtcaat ttaacacgaa ggattattcc gttcaagatg accacacttt acgcctttat
     5701 aacactactc tggatatggg tgaaatcaat aaagcacttg taatgcagaa tgtaactgtt
     5761 attagttctc aactttgtaa tgatacttta gaggactact ttaaaaaaat cacaggagga
     5821 gagggcattg cttaaactta ttcaaacaga atttcttaaa ctgcgtcgta ggaaacttat
     5881 ttggctgatg ttgttgtctg ctctcattat gccgtttttc gcactactat acttcaatta
     5941 ttttggtaaa acaggtgttg agccaacaaa gttttataaa tggtctgcat ttggttatac
     6001 actttggatt attttgcccg ttgttttggg tatattttgc accatgctta tacacgaaga
     6061 aaaccagtat gatatgctca aacagctttg gattgtacct attagtaaaa tgggatactt
     6121 ttttagtaaa tttttcgttg ttttgattta ctctatttgt tttatgctga ttacagccgt
     6181 tgcttctgtg ttatttagtg tactttctgg atatgtcgta tttagctggg caagcatttt
     6241 atatttgttg aaaaaatgtt tagaaattgg tgtaatcact gcttttgtaa tgttgccgat
     6301 tttggcaatc gctacatctc aaaaaggata cattctccca atttgtatca cattggtata
     6361 tgccttttcc ggttttgtta taatgacaat aaatatgtat ttacacccat tatccagtat
     6421 gggagttatt atcatgagaa ataaagacat tccaggtctt atattttcac aggaaattaa
     6481 tgtcccatta gcatttcttt gtatttgtat ttggagtatt gtatcagtat tgtttgcaag
     6541 cattgcacta ggaagaagaa agtgaggtgt agataatgtg gaaattactt tgggctgaat
     6601 gtcaaaaaat tcgtcgttca aaaattgttt ggattacatt ttttgcatcc attatgatag
     6661 cagtcatagt ttttgcagga gggcaggatg tatataatga cccagattta cattatggct
     6721 taaagagcgt ttatgacggt tcacggtata tagacaatgc aggttggtat atggacgcgg
     6781 tacaaccttg gtcaactttt tttgttcttc ctgctgtcat tgccctatta ggaagctata
     6841 ttatttgtcg tgaagaagag gaggacacta ttaaatccct gcgtcttatt cccataaatg
     6901 aaacaaaatt aaccatagca aaaatgatta ttacttttat attcagtata cttctttatt
     6961 tgttactctt tactataaca tttttgactg aatccgtttt acatttttcc gatttatcga
     7021 caaaactggt tttaagttgc atgaaagagt attttcttga tggcataggt gtattctttg
     7081 caatttctcc cattattgcc ttggtatcgc gcatgaaaaa aggatattgg cttgcattag
     7141 tattcacaga agtttattct attgctggac tatttgcggg tatgtcaaat gtcttacaaa
     7201 ccttttttcc tattgcagca atatttaata tttctggata ctatataact actggagaaa
     7261 aaattatggg gagtatcata agtttattac tttgtgcctg cctttctgca tttatactga
     7321 aagggctaaa acacaatgaa aaaaattgaa ataggtatat caaaatgaaa aagataaaaa
     7381 aactgattat tctaattgta ttcttgtttc tttttggcat gttacactgt tatactgaat
     7441 ggaaattcaa caatgaccca cctaaagggg taatagaaag aataaatgag tggactgata
     7501 agattggact ggattagtaa cttatgttaa ttatagtgaa tgtaatatta gcaagttatt
     7561 gtttcgtatt ttaatactat aactttaaat atttggtttt aatcttccga acgaatataa
     7621 aaaaactgtc acagaggcaa catatttttg aatatttata aagtggtagt tttgattttc
     7681 aaagctgccg ccttttcttc tatataagca aacttatcct caacaaagtg taaaacatgg
     7741 tacattttaa gaaaaaagta ccacagacac aagcaataaa agaaacgccg agaacctaca
     7801 aaaacgtagt gcttatgcag atttacgacg ccttctattt tctcaaccgt cttaatgacc
     7861 ttgtacaaaa gaaatttttc taatattttt tataatctta tcttgtgatg ttttttatgt
     7921 ataaatgact taataacaaa tccaataaaa attatggata ttggtataat tagaacgagg
     7981 aatttataat cattataatg tgacattatt cttacccaac tctcagaaaa aatatatcct
     8041 atactgatta atgaagtatt ccatatggtt attcccacga tagatgatat tacaaaagtt
     8101 ataatatttt gacctgctat acctgctata aaagatatgt atgttctaca taaagggatt
     8161 aatctacata cacaaacaga aatagaagag tatttattaa aataatcttg agatgcaaat
     8221 attcctttct ttgcctttgg acatatctct attattttat ttatgataga tttacctccc
     8281 caatttccca ctgtataaca aaatatcgaa ccaagtatac ctgcaattat acttaaaatt
     8341 aaaattgtca gaaatccaaa atgatttctt gcagcaattg ctccacataa aggtaaaaca
     8401 acttcgctag gtaatggaaa acaagcatac tcaatcataa ttaatacaaa tacagagatt
     8461 aatccatatt gtaaaataaa actattgatt acagacactt acttatctat ctcctttctt
     8521 atttaaaggt tttaagggta catattctaa ggattctcac cctatattta aagaatcaat
     8581 ctctttaaac tattatatgt atttacacaa agcatttata acatacttta aaaatagtac
     8641 cttaaaataa gaaacttaat aaataaaaaa a
//
LOCUS       DAMJWP010000152         8666 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.157, whole genome shotgun sequence.
ACCESSION   DAMJWP010000152 DAMJWP010000000
VERSION     DAMJWP010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8666
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.157"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            75..917
                     /locus_tag="PC700_002699"
     CDS             75..917
                     /locus_tag="PC700_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HDF2581996.1"
                     /translation="MKEKAGIRNAIIAAFIITILAELIGPISFKVMGINVTLLSILWA
                     IFIGMAVSPHLLGRVIPALKKFIGDNEINVSPYLLSLTLYPLGIMFGINAGPKVGILL
                     QAGPALLLQEFGHMGTMLIALPVGVALGLGRSALGGTFSLCRDTALGIIGDKYGLNSP
                     EGIGTLGTYISGSIFGTLLFSFLAPVGVLLGFHPYALAMASGMGSGSMMGAATASLMN
                     TVPQMSEQILAYSATSGLITAVTGIYIELFLSLPVANYYYSKVAPVIERFRGRKTRRT
                     SNKL"
     gene            932..1396
                     /locus_tag="PC700_002700"
     CDS             932..1396
                     /locus_tag="PC700_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2581997.1"
                     /translation="MEKTFKTIMEQIKIVLFIYIITCFGQMIALKTQAQDLLIGSISG
                     FLLVIIAIIIKNFVKKPNLPGFAWATLVAFVLSLPISPVGDIIATNVGKINFMSTVTP
                     LLAFAGISVGNQLDILKTLSWKLVVISFIVMTSTYFGSAFISQTVLRLNGML"
     gene            1383..2555
                     /locus_tag="PC700_002701"
     CDS             1383..2555
                     /locus_tag="PC700_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2581998.1"
                     /translation="MECFKEVVNMNELKAKIKEEIKLLSEEKKTSFEKVSDYLFSNPE
                     LAFEEYKSQKALCDLLEENEFNVTKGVGGLETSFEAVYSNGTNGKTVAFLAEYDALPG
                     MGHACGHNIIGTSSVGAGIILKEIMKKHNIEGTVKVFGTPAEERVGGKITMIKEGVFN
                     NVDAALILHPSDASMPDDISFAQVNLKFDFTGKASHAAAFPWEGKSALSGVIALFNSV
                     NSMRLHLKDYARVHGIITDGGSIHNIIPEKSTAIFNVRALSIEYLNEICEMLKNCAKG
                     AAISTGTNVEIVQLDEIYKEIKNDSELVNIVRQNFEVLGEDYVERDLSQGIGSTDTGN
                     LTHEIPAIQAYIKLKENTATHTDEFAVAAGGEEGKVALIKAIKVLAMCGVDILYSK"
     gene            2599..3534
                     /locus_tag="PC700_002702"
     CDS             2599..3534
                     /locus_tag="PC700_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1177 domain-containing protein"
                     /protein_id="HDF2581999.1"
                     /translation="MILKQVIEVYDILDKANANGEEVKTYLQGYGEVDVTVKELSSSK
                     GSTDLVKLTIPGKNGKLKGGDAPTLGILGRLGGIGARPEVIGFVSDGDGALVAIAVAA
                     KLLDMQRKGDILDGDVVISTHICPDAPTKEHYPTPFMDSPIDMMTMNENEVDSSCDAI
                     LSIDTTKGNRVINTRGFAISPTVKEGYILKTSDNLLDIMQTVTGKSPFVFPLSIQDIT
                     PYGNNLYHLNSILQPAVATDAPIVGVAITTEMPVAGCATGATNFSDLDSAGRFAIEVA
                     KLFGRNKCDFYDKEEWEMLIKRYGKLNRFQTFGIQ"
     gene            complement(4333..4731)
                     /locus_tag="PC700_002703"
     CDS             complement(4333..4731)
                     /locus_tag="PC700_002703"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDF2582000.1"
                     /translation="MKWKELYNNARGKLNPRTISPFIDAGGVAAAILTDSGNVYTGVC
                     IDTACTLGMCAERNAIANMITNGESKITKLVCVMSNGSVGSPCGACREYLMQLDKDSA
                     CIEILVNVETEKTVKLSELLPDWWGSDRFI"
     gene            complement(5030..7681)
                     /locus_tag="PC700_002704"
     CDS             complement(5030..7681)
                     /locus_tag="PC700_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HDF2582001.1"
                     /translation="MNNDNDKNGNKIRRKKVSSSGNTNKPINRTSANKTRSTKNKKKS
                     KKSDKFKKLRVFGIVFLVLLVVGTAGTAGLVFASLRDVTPVTEAVLDKQTNQTTTIKY
                     ANGKTLSTAPSVNKKTPVPLDKISPYLQHAVIAIEDERFYEHKGVDIKGLFRSVLKTL
                     TGTKQGGSTIPMQVSKMLLTTEQQTIPRKIKDIYYAHEMSKTVSKDKILETYLNNFFV
                     GRGLAGAEAGARGYFDKSAADLTLAESALLAGSTKNPSRFSAYKTSKLEGNETKEDLE
                     NKLLFFVNTTDDDLDDPTQVDFDMIEKIKSWELISNDTYRQLKAGTLVVRKAVSNPEA
                     KKRQEIVLKKMLELKYIKQSEYDEAIKAKIEIKLPQSSDKVSSSVEDLIESEVINALM
                     EQGHTNDEAQNLFYNGGLIVNTTIDPKMQDALEEEFDRNSNFPGHMVGPDGVSQPQAA
                     MVILDYKNGEIRALAGGRNISGRKTLNRATNPHQPGSSIKPLAIYTPAIDTLKITQST
                     ALSDSRGGYKFEENNKWNPRTTTAGHGSMSLRKALAKSSNTIAVKTAEMLGDSYDECV
                     DIMMDYLKNFGITTLKNNHSGSSEASDRKFPSLTLGGMANGITPLQMAAAYGTLANQG
                     VYVEPSIFTTITTFDGQLLVKNAPEEHKVVDPEVAYVVTDMLESVITEGTGGVATLPK
                     GMPVAGKTGTTNSAYDAWFVGYTPYYVGATYIGDDAGRKDDSGNTIKRREVPHGSTST
                     AKLWEKIMEKIHANLTVTEFEVPKNVYFTKINLEDGGKQSSGSKAAFIEGTAPTKVSS
                     QPSSEDTKKPDNNQPEENNNNNNNTGDNGGGSTPPDNGGNNGGGSTTPPDNGGNNGGG
                     STTPPDNGGNNGGGSTTPPDGGGTPAT"
     gene            7895..8593
                     /gene="yunB"
                     /locus_tag="PC700_002705"
     CDS             7895..8593
                     /gene="yunB"
                     /locus_tag="PC700_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HDF2582002.1"
                     /translation="MNRIVDRKTKNEFRKIIAIFLVILFLSVFIGSFIYIDKTLRPTI
                     TVLAETKALELANRSINKAVAEMVEGKINYEDLMDIQLDNNGKITMIQANTIMMNEIA
                     SAIALEIQDELKKDKTASSYIPIGTALGSPILAKYGPKLEVSIEPIGTVSVNFKTEFE
                     SSGINQTRHRIYLEAQTQVKVVIPLITSTKQIKAQIPICETIIVGDVPESYVNIPEKN
                     LGNVLPNTGKNTNK"
BASE COUNT         2940 a         1341 c         1509 g         2876 t
ORIGIN      
        1 atgataggtt atataataaa atcactaaca attatattat aagaaaatta taaaaattac
       61 aaggaggtat agaaatgaaa gaaaaagcag ggattagaaa tgccataata gcagccttta
      121 taatcacaat attggcagaa ttaattggac ctatttcatt taaagttatg ggaataaatg
      181 taactttact tagtatacta tgggctatat tcataggaat ggctgtttct cctcatttat
      241 tagggagagt tataccagcc ttaaaaaagt ttatagggga taatgaaata aatgtatctc
      301 catatttatt atctttgaca ctatatccat taggaataat gtttggaatt aatgcagggc
      361 ctaaagttgg tatattactt caagcaggac cagcactttt attacaagaa tttggtcata
      421 tgggtacaat gttgatagca ttacctgttg gagtagcatt aggtcttgga agaagtgcat
      481 taggtggtac tttttcatta tgtcgtgaca cagcactagg cattattgga gataagtatg
      541 gattaaactc accagaaggt ataggaacac taggaactta cataagtggt agtatatttg
      601 gaacattatt attttcattt ttagcaccag tgggtgtact tttaggattt catccatatg
      661 ctttagcaat ggcatcagga atgggtagtg gttcaatgat gggagctgca acagcatcat
      721 taatgaatac ggttccacaa atgtcagagc agattttagc atattcagct actagtggat
      781 tgattacagc tgttactgga atatatatag aattattctt atctttacca gtagccaact
      841 actattatag caaagtagct ccagttattg aaagatttag aggaagaaaa acaagaagaa
      901 caagtaataa attgtaaagg aggatatata tatggaaaaa acttttaaga ctattatgga
      961 gcagataaaa atagtattgt ttatatatat tattacttgc tttggacaaa tgatagcact
     1021 taagactcag gctcaagatt tgttgattgg aagtatatct ggatttttgt tggtgataat
     1081 tgctattata ataaagaatt ttgttaaaaa acctaattta ccaggttttg catgggctac
     1141 attagttgca ttcgttttat cgcttccaat atctccagtg ggggatatca tagcaacaaa
     1201 tgtagggaaa atcaatttta tgtctacagt aacaccatta ttagcttttg caggaatctc
     1261 tgtaggtaac caattagata tattaaagac tttgagttgg aagttagtag ttatatcatt
     1321 tatagttatg acatctactt actttggttc tgcttttata tctcaaacag ttttaagatt
     1381 aaatggaatg ctttaaggag gtagtaaata tgaatgaatt aaaagcaaaa attaaagagg
     1441 aaataaagtt attatctgaa gagaaaaaga catcatttga aaaagtttct gactatttat
     1501 tttcaaatcc agaattagca tttgaggagt ataagtcaca aaaagcatta tgtgatttgt
     1561 tagaagaaaa tgaatttaat gtaactaaag gtgtaggagg attagaaaca tcttttgaag
     1621 ctgtgtattc aaatggaact aatgggaaaa cagttgcttt tctagctgaa tatgatgcac
     1681 ttccaggaat gggacatgca tgtgggcaca atatcatagg gacaagttct gttggtgcag
     1741 gtatcatatt aaaagaaatc atgaaaaaac ataatataga agggactgta aaggtttttg
     1801 gaacacctgc agaagaaaga gtaggtggaa aaataactat gattaaagaa ggtgtattta
     1861 ataatgtaga tgctgcatta atcttacatc caagtgatgc ttcaatgcct gatgatatat
     1921 catttgctca agttaattta aaatttgatt ttactggtaa ggcatcacat gctgctgctt
     1981 ttccttggga agggaaaagt gccttaagtg gagtaatagc actatttaac agtgtaaata
     2041 gcatgagatt acatcttaaa gattatgcaa gagtacatgg aattataact gatggaggta
     2101 gcatacataa tataattcct gaaaaatcta cagctatatt taatgtaaga gccttaagca
     2161 tagaatattt aaatgagatt tgtgaaatgc ttaaaaattg tgctaaagga gctgcaattt
     2221 ctactggaac taatgtagaa atagttcaat tagatgaaat atacaaagag attaagaatg
     2281 attctgaatt agtgaatata gttagacaaa attttgaagt tttgggtgaa gactatgttg
     2341 aaagagattt aagtcaaggt ataggttcaa ctgatacagg aaatttaact catgagatac
     2401 ctgcaataca agcatatata aaattaaaag aaaatactgc aactcataca gatgaatttg
     2461 ctgttgcagc tggtggtgaa gaaggcaaag tagcacttat aaaagcaatt aaggtgctag
     2521 caatgtgtgg agtagatatt ttgtatagta aataatatat aattttaaaa tatattaaga
     2581 aaggaaatcg gattagacat gatattaaaa caagtaatag aggtttatga cattttagat
     2641 aaagcaaatg ctaatgggga agaagtaaaa acttacttac aaggttatgg tgaagtagat
     2701 gtaactgtaa aagaattgtc ttcaagtaaa ggttcaacag atttagtaaa gcttacaatt
     2761 cctgggaaaa atggaaaatt aaaaggtgga gatgctccta ctcttggtat tttaggtaga
     2821 ttaggaggga ttggagcaag accagaagtt ataggttttg tctcagatgg tgatggagca
     2881 ttggtagcaa ttgcagtggc tgctaaactt ttggatatgc aacgaaaagg ggacatatta
     2941 gatggtgatg ttgtaatttc aactcatatt tgcccagatg ctccaactaa agaacactat
     3001 cctactcctt ttatggattc tccaattgat atgatgacta tgaatgaaaa tgaagttgat
     3061 agtagttgtg atgcaatact ttctatagat actactaaag gaaatagagt aatcaatact
     3121 agaggatttg caatatcgcc aactgttaaa gaaggctaca tattaaaaac tagtgataac
     3181 cttttagata ttatgcagac tgtaacaggg aaatctccat ttgtattccc tttgtctatt
     3241 caagatataa caccatatgg aaacaatctg tatcacttaa atagtatttt acaaccagca
     3301 gtagctacag atgcaccaat agtaggagta gcaattacaa cagagatgcc tgttgctgga
     3361 tgtgctacag gtgcaactaa ttttagtgat ttagattcag ctgggagatt tgcaattgaa
     3421 gttgccaagc tatttggtag aaataagtgt gatttctatg ataaagaaga gtgggaaatg
     3481 cttattaaga gatatggcaa attaaataga tttcaaacat ttggtataca gtaattagaa
     3541 aaatattaag tttatataat ttgtgataca actatttaag aagatataaa caattaggaa
     3601 taaaagtaag aataaagcac ttagttaaaa actttaaaaa aaaatattta tttgtttata
     3661 taaaaaggag gattctggtt attgggattc tcttttttat gaatacaaat aatatattgt
     3721 aattataaaa aaaggagcgt ttcaaagtga tttttttaat caccttgaga caactcctta
     3781 tattttttat ttaaattcta ttttttattg actgtatagc tattttcacc atcataaggt
     3841 ggcatgacct tgaacagcgt ggttttgtaa agttgggcac cacaaaacaa aaaatcaaca
     3901 agcccttgtg tggtctgctg acgtgtttac tatactatac tttcccaaaa accgcaagca
     3961 ctatgggagg ctataaggcg gcgaccattc aggggcgatc ccctcgaaac cgggataggg
     4021 ttcccatgtg ggacataata aagagcgccc cggaggtatc aactaccatc cggggcgctc
     4081 tttattatgt cccgtgggaa ccatgcccac gaagcagggc gtacaacgga cagataggtc
     4141 ctaccctcct gacagactgg aatctcaatc ccatcgaaaa caatcataac aatcatggca
     4201 agattatata cactcctgta aaatattttt gtgtgtacca tgaagtactg cttgccacta
     4261 ttgagaacgg ataaaaggga agtacgtatt ataccaataa ggtgaacgaa acaaaatact
     4321 catttgataa agttaaataa acctgtcgga accccaccaa tccggcaaga gctcactcaa
     4381 tttaacagtt ttttctgttt cgacattaac taaaatctca atacaagcac tatccttatc
     4441 aagttgcatt aaatattcac gacaagcacc acatggtgag ccaacactcc cattgctcat
     4501 cacacaaact aatttagtaa ttttactttc gccatttgtt atcatattgg cgatagcatt
     4561 tctttctgca cacattccca atgtacaggc tgtatcaata cacacgccag tataaacatt
     4621 tccgctgtcg gtaaggattg ctgcggcaac cccaccagca tcgataaaag gtgaaattgt
     4681 gcgtggattt aattttcccc tagcattatt ataaagttcc ttccatttca tcaggttgat
     4741 cctcctgttg tatccatata aataatcttt tatacgcctt ttccgcctaa ggcacacaac
     4801 acattactcg cttttttcat tctaatattc gtgcgtgaaa atgtcaaacg gtttacttcg
     4861 tttcattact tcatactgct gttcataacc ctgtttgttc acttgggcac cattcgagca
     4921 ccaatttacc cgtttcaggg ttgtagcgaa cacagcagaa atgctcattt catgggcttt
     4981 tctggctaga aaaaaaattt tatcgctgcc agatatttac tattttttat taagttgctg
     5041 gagttccacc accatcagga ggagtagtag agccaccacc attatttcca ccattatcag
     5101 gaggagtagt agagccacca ccattgtttc cgccattatc aggaggagta gtagagccac
     5161 caccattgtt tccgccatta tcaggaggag tagaaccgcc accattatca cctgtgttat
     5221 tattgttatt attattttct tctggttggt tattgtctgg tttttttgta tcttcagatg
     5281 atggttgaga acttacttta gtaggagcag taccttcaat aaatgctgct ttagagccag
     5341 aagattgctt tcctccatcc tctaaattaa tttttgtaaa atatacattt ttaggaactt
     5401 caaattcagt aaccgttaaa ttagcatgta ttttttccat aattttttcc catagttttg
     5461 cagtactagt acttccatgt ggtacttctc tacgttttat agtgttacca gaatcatctt
     5521 ttctaccagc atcatctcca atataagttg caccaacata ataaggagtg taaccgacaa
     5581 accaagcatc atatgcacta tttgtagtac ctgttttacc tgctacaggc attccctttg
     5641 gaagtgtagc aacaccacca gtaccttcag taattacgct ttctaacata tctgtaacaa
     5701 cataagctac ttcagggtct acaactttat gttcttcagg agcattttta actaataatt
     5761 gaccatcaaa ggtagttata gtagtaaaaa tagaaggttc tacataaaca ccttgatttg
     5821 ccaatgtacc ataagcagca gccatttgaa gtggagtaat accattcgcc ataccaccta
     5881 gtgtcaatga tgggaatttt ctatcacttg cttcagatga acctgagtga ttattcttta
     5941 aagttgttat accaaagttc tttaagtaat ccatcattat atctacacat tcatcataag
     6001 agtctccaag catttcagca gttttgacag ctatcgtgtt tgaagattta gcaagtgctt
     6061 tacgtaaact catagaacca tgaccagcag tagtagttct tggattccat ttattatttt
     6121 cttcaaactt atatcctcct cgagaatcac ttagagcagt tgattgtgtt atttttaggg
     6181 tatcaatagc aggtgtatat attgctaaag gttttataga agaacctggc tgatgagggt
     6241 tagtagcacg attcaaagtt tttctaccac ttatatttct tcctccagct agtgctctta
     6301 tttcaccatt tttataatct aagattacca ttgcagcttg tggttggcta actccatcag
     6361 ggccgaccat atgtcctggg aaattgctat ttctatcaaa ttcttcttct aaagcatctt
     6421 gcattttagg gtctatagtt gtgtttacaa ttagaccacc attgtagaat aagttttgag
     6481 cttcatcatt tgtatgtcct tgttccatta aagcatttat aacttcactt tcgataaggt
     6541 cttctacaga tgaagaaact ttatctgatg attgaggcaa ttttatttct attttagctt
     6601 tgattgcttc atcatattca ctttgtttta tatatttcaa ttctaacatt ttctttaaaa
     6661 caatttcttg tcttttttta gcttcagggt tacttacagc ctttctaaca actaatgtac
     6721 ctgcttttaa ttgtctataa gtatcattag atataagctc ccaagatttt attttttcaa
     6781 tcatatcaaa gtccacttga gtagggtcat ctaaatcatc atcagttgtg ttaacaaaaa
     6841 ataataattt attttctaaa tcctccttag tttcatttcc ctctaactta gaagttttat
     6901 aggctgaaaa tcttgatgga tttttagttg aaccagcaag aagtgctgac tctgctagag
     6961 tgaggtcagc tgcagactta tcaaaataac cacgtgcacc agcctcagca cctgctagac
     7021 ctctaccaac gaagaagtta tttaaataag tttctagtat tttatcttta cttactgttt
     7081 tactcatctc atgtgcatag taaatatcct ttattttacg aggtatagtt tgctgttcag
     7141 ttgttaacag cattttggat acctgcatag gaatagtact accaccttgc ttagtacctg
     7201 taagggtctt taatactgac ctaaataaac ctttaatatc gacaccctta tgctcataaa
     7261 aacgctcatc ttctattgca attacagcat gttgaagata aggggaaatc ttatctaatg
     7321 gaacgggtgt ctttttattt acacttggag ctgtagacaa tgtttttcca ttagcgtatt
     7381 taatagtagt tgtttggttc gtctgtttat ctaaaacagc ttctgtaact ggagttacat
     7441 ctcttagtga cgcaaatact agaccagcag tacctgctgt accaacaact agtaaaacta
     7501 aaaatactat cccaaatact cttagttttt taaatttatc gcttttttta gattttttct
     7561 tgttttttgt tgaacgagtc ttgttagctg aagttctatt aattggttta tttgtattac
     7621 cagatgagct aacttttttt ctcctgattt tatttccatt tttatcatta tcgttattca
     7681 taagatttta acctccttac ttaatgcaat attataacac aaataggagt agttttgtaa
     7741 ttaatagaaa aaatgaattt gctataatat tctgtacatt gtatatgtat aaacgtaata
     7801 tattaaaaag tctatatttt tattttattt atttttgaca aatattataa aaattatgcc
     7861 aaagctacat ataaataaat aggaggggat aattttgaat agaatagtag atagaaaaac
     7921 aaaaaatgaa tttagaaaaa tcatagctat ttttttagta attcttttct tgtctgtatt
     7981 tataggcagt tttatatata tagataaaac tttaagaccg acaataacag ttttagcaga
     8041 gactaaagct cttgaactag caaataggtc gataaataaa gctgttgctg agatggtaga
     8101 aggaaaaata aattatgaag atttaatgga tatacaactt gataataatg gtaaaataac
     8161 tatgatacaa gcaaacacta taatgatgaa tgaaattgca tctgcaatag ctttagaaat
     8221 acaagatgaa ttaaaaaagg ataagacagc aagttcttac atacctatag gaacagcatt
     8281 aggaagtcct atattagcta aatacggtcc taaacttgaa gtgtctattg aacctatagg
     8341 aacagtatca gtaaacttta aaacggaatt tgagtcttca ggtataaatc aaactagaca
     8401 tagaatatat ttagaagctc aaacacaagt taaagtagta ataccactta taacttcaac
     8461 aaaacaaatt aaagctcaaa tacctatttg tgaaactata atagtaggtg atgttccaga
     8521 aagttatgta aatattccag aaaaaaatct aggaaatgtt ttacctaaca ctggaaagaa
     8581 taccaacaag taatataaag ttgtatattt ttaaataaat tcaaaataat agtttataaa
     8641 taaaattttt ggaaataata ttaata
//
LOCUS       DAMJWP010000153         8469 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.158, whole genome shotgun sequence.
ACCESSION   DAMJWP010000153 DAMJWP010000000
VERSION     DAMJWP010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8469
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.158"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            16..2193
                     /locus_tag="PC700_002706"
     CDS             16..2193
                     /locus_tag="PC700_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2582003.1"
                     /translation="MYNKYNNLYLLLGKENVNMDSYRQLKAKIPCKETGIEIKRTLCD
                     ICSPGVHCGIDAYVKDGKVIKIEGTKEHPMNKGLLCTKGLCNREYIYREDRIKTPLKR
                     IGKRGEGKFKPISWDEAYREIAEKLNRVKSEYGAKHVAFFTGYGKWYRPFLQRFASSF
                     GSPNYGTESSSCSTSAQMAGNVATGRNFTMQDMENAKTYLGWGFNPYYSKFLNSVNLI
                     KLKEKGLKFIIVDPRITPTTQKLADLHLRIKPGTDGALALGMANLFIQKDLIDREYIE
                     KHVHGFNEYKEYVKLFDLDTVSRITGVSKDNIVKAVEMLSQGPFCINESAAPIVHHQN
                     GFQNYRAMMSLSAITGNYDRIGGNIPKQDYFAGKIGGFQTLEKEFVKEVLPKNINERI
                     GGERFPLWNEMVTQFQAMDLPRQIEEKTPYEIRALFCHGINARMFPNSGRLFKALHSV
                     DFFVNIDLFMTDTSKYADILLPACTSFEREECKSYPGGYITYTKKVIDKLYDSKSDVE
                     ILSDLANVMNIDDDLLKAGYEASIRHMFKKTCVDVDKLKESDLPLKCKDFKPYIVGSY
                     TREGYDTSTSRFELKSTIIEKYKDFGLDALPTYRGFNDNIDNEYTHLLTSGARISNAL
                     HSRLHDIKLSRILKNEASCDISSLDAKELGVISGDYIKITTKIGSLDVKVNVTDRVLY
                     KNIHMYHGYRESDINSIIDDNFDPYSGFPAYRSCKCKIEKMNR"
     gene            2207..2629
                     /locus_tag="PC700_002707"
     CDS             2207..2629
                     /locus_tag="PC700_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2582004.1"
                     /translation="MKSLFKFNKELCTACGACLLACIDQNDNNIDNGELFRHVFTIEE
                     GENIEYFSLACIHCNEPKCLGACPKNCFKKEEGFVVLDNQNCIGCKLCEKACEYGALI
                     YGNDGKANKCNGCIERYKCGMDSPCVMVCPTGALKFNK"
     gene            3036..3839
                     /locus_tag="PC700_002708"
     CDS             3036..3839
                     /locus_tag="PC700_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDF2582005.1"
                     /translation="MKKLKLIIMLALVLALLITGCSSGNNKNKEEQKTIRVATSGTYY
                     PFAFKDKDKLKGFEVDFWDEFAKRTNCKVEWVLTDFSGLFGLLEADKADVVSAQLTAT
                     PEREKKYAFSDAYNYSGTNIIVREDDTSIKSIDDLVGKKVGVGTGAVANEILKEKYPN
                     DEIKIVNYSSATLKGNYQDLEMGRLDAVVAQDVEALIAIKEQKLNLKMVEPPIQFGAC
                     SFAIQKNEKGEKWTKEINEVIEEMHKDGTLTKISEAWLGKDITKEPINE"
     gene            3832..4500
                     /locus_tag="PC700_002709"
     CDS             3832..4500
                     /locus_tag="PC700_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDF2582006.1"
                     /translation="MSNFNFEFCLSIFPFLIKALGITILISIVAFFLSILLGVILAFF
                     TQTKSKVLQYITRVYVSFFRGTPLLIQIFFFYFGFPMIFSFMKDCSPYLAIVICLGVN
                     SSAYMTEIIRASIESVDKGQQEACYAFGLSKYQSMVKIILPQAATSAVPPIVSCTLDI
                     IKGSSLGLTIGIQDLMGVAQVEAASSFKYFEIYMITILIYWAVSIILSNIQSKVEKRI
                     GKAY"
     gene            4736..5398
                     /locus_tag="PC700_002710"
     CDS             4736..5398
                     /locus_tag="PC700_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDF2582007.1"
                     /translation="MIFNMKYFLELFPIIMKSFNVTVILAISSLVFSLIIGTIVALSS
                     YYKVKVLNQICKVYVSIFRGTPLMPQLFFLYFGLAYMNDFVKNMDPVFATSIVLSLNM
                     GAYMSETIRASILSIDKGQIEAAYVMGLTNLQTMKRIVIPQAVRVALPSLFNNFIDLI
                     KGSSVAFVVGVNDIMGAAKSQGALSFKFFEVYGAVMVIYWCIITPLGWGQKKLEKKIS
                     NY"
     gene            5458..6255
                     /locus_tag="PC700_002711"
     CDS             5458..6255
                     /locus_tag="PC700_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2582008.1"
                     /translation="MVKRNIIKCLVAVLSLTFVVAGCSKKEEVKDEKVITVASSNSAD
                     PYSYVDNGEHKGFEVDMWKEIGKRSGYKIKMEPTGFSSIFGMLDSGKVDVAANFFGMS
                     KERLEKYDASIPYGSDSVCIAVKDGNNEINKLEDLKGKVVAVSEGSQGQQVAASVNKD
                     NLFEEKVYGDGTNGLQDLDLGRVSAWIDAELTVVGDAKKANMKVRVLDEKLSVTNIAY
                     FFKKNDEKSKTIKEDVNKAIEEMLSDGTVKKISEKWFGMDVSADIQK"
     gene            6388..7563
                     /locus_tag="PC700_002712"
     CDS             6388..7563
                     /locus_tag="PC700_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2582009.1"
                     /translation="MIETKIKSIALKNREKIIKIRRQIHSNPELAFKEFKTSKLIKEE
                     LNKLKIEYIDVAGTGVLATIKGKNNGGKTILLRADMDALPIKEENDLEFKSINDNMHA
                     CGHDAHVSWLLGTAMILNDIKEELNGNVKLLFQPGEEKGGSDIVIKENVLEGVDALAT
                     GHCWPTIESGKIGIARNCAMAATNTFEITIIGKGGHGAEPHNCIDPIAVGNAVYSSIQ
                     QIVSRKIDPVVPVVVSVCSFNSGVSKNIIPDVCTLQGTIRAISKEQVIEVSKILENIV
                     RNVCRAYGADCKFEKSTGGDAVINDKNMIELGRESACKILGVENVEIIDFPAMTGEDF
                     AIYMKEKPGLFMYIGVGNKEKNINYRLHSNKFNIDEKCLSIASSLFSQLAVDYLCQL"
     gene            7672..8421
                     /locus_tag="PC700_002713"
     CDS             7672..8421
                     /locus_tag="PC700_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDF2582010.1"
                     /translation="MIKVENLHKSFKGTEVLKGINLEINQSEVVAILGPSGTGKSTLL
                     RCLNYLVEPTKGKIMIGDVKVDVEKATKKDIANLRKHTSMVFQNYNLFKNKTALENVM
                     EALIVVHKKSNKEAREISLKLIDKVGMLDRKDFYPSKLSGGQQQRIGIARAMAVNPNV
                     ILFDEPTSALDPELVGEVLSVIKSLAKEGITMLIVTHEINFAKEVADKIIFMDNGVIA
                     EEGTPDEIFNNPKNERTAKFLNIVRKEEVFS"
BASE COUNT         3356 a          863 c         1580 g         2670 t
ORIGIN      
        1 atttgaataa aaagaatgta taataagtat aataatttgt atttattatt gggaaaggaa
       61 aatgttaata tggatagtta tagacaactt aaagctaaaa taccctgtaa ggaaacaggt
      121 atagaaataa agaggacttt atgtgatatt tgttctccag gagtacattg tggaattgat
      181 gcatatgtta aagatggtaa agtaataaaa atagaaggta caaaagaaca tccaatgaat
      241 aaagggttgt tatgtaccaa aggtctttgc aatagagaat atatatatag agaagataga
      301 ataaaaacac ctctaaaaag aattggaaaa agaggtgaag gaaaatttaa accaatatca
      361 tgggatgaag cttatagaga aatagcagaa aaattaaata gagtaaaatc tgagtatgga
      421 gcaaagcatg tagctttttt tacgggttat ggaaagtggt atagaccttt tttgcaaagg
      481 tttgcatctt catttggttc acctaattat ggtacagaat ctagttcatg ttcaacatca
      541 gcacagatgg ctggaaatgt tgctactgga agaaacttta caatgcaaga tatggagaat
      601 gcaaaaactt atttagggtg gggatttaat ccatactact ctaaattttt aaatagtgtg
      661 aatctaataa aattaaaaga aaaaggtcta aaatttataa ttgtagaccc tagaataaca
      721 ccgacaactc aaaaactagc agatttacat ttaagaatta aacctggaac tgatggagct
      781 ttagctcttg gaatggcgaa tctatttata caaaaggatt taatagatag agaatacata
      841 gaaaagcatg tacatggatt taatgaatat aaagaatatg taaaattatt tgatttagat
      901 actgtaagta gaattacagg agtttctaaa gataatatag taaaagctgt tgagatgttg
      961 tcacaaggtc cattttgtat aaatgaaagt gcagctccaa tagttcatca tcaaaatgga
     1021 tttcaaaact atagagcaat gatgtcttta tctgctataa caggtaatta tgatagaata
     1081 ggtggaaaca taccaaaaca agattacttt gctggtaaga taggtggatt ccaaacacta
     1141 gaaaaagagt ttgtaaaaga agttctacct aaaaatataa atgaaagaat tggtggagaa
     1201 agatttcctc tatggaatga aatggtaact caatttcaag ctatggattt acctaggcaa
     1261 atagaagaaa agacaccata tgaaataaga gctttgtttt gtcatgggat aaatgctaga
     1321 atgtttccta attcagggag actttttaaa gcgttgcata gtgtagattt ttttgtgaat
     1381 atagatttat ttatgacaga tacatcaaaa tatgcagata tacttttacc tgcgtgtaca
     1441 tcatttgaga gagaagaatg taagagttac ccaggagggt atattacata taccaaaaaa
     1501 gttattgata aattatacga ttcaaaatca gatgtagaaa tactttcaga tttagcaaat
     1561 gttatgaata tagatgatga tttattaaaa gcaggttatg aagcaagtat aagacatatg
     1621 tttaaaaaga cgtgtgtaga tgtagataaa cttaaagaaa gtgatttacc attaaaatgt
     1681 aaagatttta agccatatat agtaggctca tatacaagag aggggtatga tactagcact
     1741 tctaggtttg aattaaaatc tacaattatt gaaaaatata aagattttgg actggatgca
     1801 ctaccaacat atagaggatt taatgataat atagataatg aatatactca tttattgaca
     1861 tcaggagcaa gaatttcaaa tgcactacat tcaagacttc atgacatcaa actttcaaga
     1921 atattaaaaa atgaagctag ttgtgatatt tccagcttgg atgcaaaaga attgggtgtt
     1981 atcagtggag attatataaa aataactact aagataggaa gtttggatgt aaaagtcaat
     2041 gttactgata gagtcttata taagaatata catatgtatc atggatatag agaatctgat
     2101 ataaatagta ttattgatga taattttgac ccttatagtg gattccctgc gtatagaagt
     2161 tgcaagtgta agattgaaaa aatgaataga taaatggaga gatataatga aaagtttatt
     2221 taaatttaat aaagaattat gtacagcatg tggagcatgt ttgcttgcat gtatagacca
     2281 aaatgataat aatatagaca atggtgaact ttttagacat gtatttacaa tagaggaagg
     2341 tgaaaatata gaatattttt cattagcatg tatccattgt aatgagccaa aatgtttagg
     2401 tgcatgtcct aaaaattgtt ttaaaaaaga agaaggattc gttgtattag acaatcaaaa
     2461 ctgtataggg tgtaaacttt gtgaaaaagc ttgtgaatat ggagccttaa tttatggaaa
     2521 tgatggtaag gcaaataagt gtaatggttg tattgagaga tataagtgtg gtatggattc
     2581 accttgtgta atggtatgtc ctacaggagc attaaaattt aataaataaa ttaaatatag
     2641 agagtgactt atacttggta taagatattt ttacaaaaag ctatattgag tttgttataa
     2701 taaaaactca atatagcttt tttatggata tagttggata ctataatgtg atttttagta
     2761 atagtttttt gctattggac aaagactgta aaacatatta aacttatgtt gttcaaagtc
     2821 gaagtataat ataataacta gaaaattgat ttatagtgtt ataaaagcaa atttagtaag
     2881 gtataaaata agaggcaatc ttttactaca gtatcaatat ttgggagtta tgttatggca
     2941 gagccattaa acaattataa tggtacaagt gattcaacct ttgaaaatgt attatataaa
     3001 aaattaaaat aagattaaaa aggagagaat aaattatgaa aaaacttaaa ttaattatta
     3061 tgcttgcctt ggtattagca cttttaatta caggatgttc atcaggaaat aataaaaata
     3121 aagaagaaca aaaaactata agagttgcaa catcaggaac atattaccct tttgctttta
     3181 aagataaaga taaacttaaa ggatttgaag tagatttttg ggatgaattt gctaaacgaa
     3241 ctaattgtaa agttgaatgg gtattaactg attttagtgg attatttgga ttattagaag
     3301 ctgataaagc agatgttgta tctgctcaac ttactgcaac acctgaaaga gagaaaaaat
     3361 atgctttttc agatgcttat aattatagtg gaacaaacat tattgttaga gaagatgata
     3421 ctagtataaa atcaatagat gatttagttg gcaagaaagt aggtgtgggt actggagcag
     3481 tagcaaatga aatactaaaa gaaaagtacc caaatgatga aataaaaata gtaaattatt
     3541 caagtgcaac tcttaaaggg aattatcaag atctagaaat gggaagatta gatgcagtag
     3601 ttgcgcagga tgttgaggct ttaatagcta taaaagaaca aaaattaaat cttaaaatgg
     3661 tagaacctcc aattcaattc ggagcctgtt cttttgcgat tcaaaaaaat gaaaaaggtg
     3721 aaaaatggac aaaagaaatt aacgaagtaa ttgaagagat gcataaagat gggactttga
     3781 ctaaaatatc tgaagcctgg ctaggaaaag acattactaa ggagcctatt aatgagtaat
     3841 tttaattttg aattttgtct aagtatattt ccatttttga ttaaagcatt gggaataaca
     3901 atactaatat ctattgttgc atttttttta tcaatattat taggtgtaat acttgccttt
     3961 ttcacacaga caaaatcaaa agttttgcaa tacataacaa gagtgtatgt ttcattcttt
     4021 agaggaacac cacttttaat tcaaatattt ttcttttact ttggatttcc aatgatattt
     4081 tcttttatga aagattgttc accttattta gcaatagtaa tttgtttagg tgtaaattca
     4141 tctgcatata tgactgaaat tatacgagca tctattgaga gtgttgataa aggacaacag
     4201 gaagcatgtt atgcttttgg gttaagtaaa tatcagtcaa tggttaaaat aattctacct
     4261 caagcagcta catctgcagt acctcctatt gtaagctgta cactggacat aattaaaggt
     4321 tcttctttag gtctaactat aggaatacaa gatttaatgg gagtagctca agtagaggct
     4381 gcttcaagtt ttaaatattt tgagatatat atgataacga tattaattta ctgggcagta
     4441 tctataatat tgagtaatat acaaagcaaa gttgaaaaaa gaataggaaa ggcatattag
     4501 atttcaatat ttaaaaatgg gatgatgtag atatattatt acttgtaaat aaatttttaa
     4561 ttttagaaaa ataaacttag taatctaaat tattgagaaa atatagtatc aaaatggact
     4621 gtataatcaa gttaaaaaat agtttttcta ttgaaaaaat aaataaaatt ctattgtaat
     4681 ttaatattcg acaaagtttt ataaaaaaca acattaagct agggggactt attttatgat
     4741 ttttaatatg aaatattttt tagaattatt tccaataata atgaaatcgt tcaatgtaac
     4801 tgtaattttg gcaataagtt cattagtatt ttcattaatt ataggtacaa tagtagcatt
     4861 atcatcttat tacaaagtga aagtattgaa tcaaatatgt aaagtatatg tatctatctt
     4921 tagaggtacg cctcttatgc cacagttatt ctttttatat tttggactag cgtatatgaa
     4981 cgattttgtt aaaaatatgg acccagtatt tgcaacatct atagtactta gtttaaatat
     5041 gggagcttat atgtccgaaa cgataagggc atctatactt tcaattgata aaggtcagat
     5101 agaagcagca tatgtaatgg gattaacaaa tttacaaaca atgaaaagga tagtgatacc
     5161 acaggccgta agagttgcac tgccatcatt attcaataat tttatagatt taattaaagg
     5221 gtcttctgta gcttttgttg ttggagtaaa tgatataatg ggtgctgcca aatcacaagg
     5281 agcgttgagt tttaagttct ttgaagtgta tggagcagtt atggtaattt actggtgcat
     5341 aataactcct ttagggtggg ggcaaaaaaa attagaaaag aaaataagca attattaagt
     5401 tattaagaaa atacataaaa gtatttttat ataaaaaatg gtgtgaggag aagagatatg
     5461 gtaaaaagaa atattataaa atgtttagtg gcagttttat cgttaacatt tgtagttgct
     5521 ggttgttcaa aaaaagagga agtaaaggat gaaaaagtta taactgtagc ttcatctaat
     5581 tcagctgacc catattctta tgttgataat ggtgagcata agggttttga agttgatatg
     5641 tggaaagaga ttggtaaaag aagtggatat aagataaaaa tggaaccaac aggttttagt
     5701 tctatatttg ggatgttaga ttctggtaaa gttgatgtgg cagccaattt ctttggaatg
     5761 tctaaagaaa gattagaaaa atacgatgct tctatacctt atggttctga ttctgtatgt
     5821 atagctgtaa aagatggaaa caacgaaatt aataaacttg aagatttaaa aggaaaagtt
     5881 gtagcagttt cagaaggttc tcaagggcaa caggttgcag ctagtgttaa taaagataat
     5941 ttgtttgaag aaaaggttta tggtgatgga actaatggtc ttcaagattt agatttagga
     6001 agagttagcg catggattga tgcagaactt actgttgtag gagacgctaa aaaagctaat
     6061 atgaaggtaa gagtattaga tgaaaaatta tcagttacta atatagcata cttctttaag
     6121 aaaaatgatg aaaaatctaa aactataaaa gaagatgtaa ataaagcaat agaagaaatg
     6181 ctttcagatg gaacagtgaa aaaaatatct gaaaaatggt ttggaatgga tgtaagtgct
     6241 gatatacaaa aataaatact ttaaaattaa aatataaaac tgtgataact atagataagt
     6301 atactaaatt tatctatagt tatctgtgta ttacaatgta tttagattag tctttataaa
     6361 ttaaaatgag ttatggaggt cttgcaaatg attgaaacta aaataaaatc cattgcacta
     6421 aaaaatagag aaaaaatcat aaaaataaga cgacagatac acagtaatcc agagttagca
     6481 tttaaagaat ttaaaacatc caaacttata aaagaagagt taaataaact taaaatagaa
     6541 tacatagatg ttgctggtac aggagtatta gctactataa aaggaaaaaa taatggtggg
     6601 aaaactatat tgcttagagc ggatatggat gcccttccaa tcaaagagga aaatgattta
     6661 gaatttaaat caataaatga taatatgcat gcttgtgggc atgatgccca tgtttcatgg
     6721 ttgttaggaa cagcaatgat tttaaatgac ataaaagaag aattgaatgg aaatgtaaaa
     6781 ttactatttc aacctggcga agaaaaaggt ggttctgata ttgtaattaa agaaaatgta
     6841 cttgaaggtg tggatgcatt agcaactggt cattgttggc caactattga atcaggaaaa
     6901 attggaatcg ctagaaattg tgctatggca gcgactaata cttttgaaat aacaataatt
     6961 ggcaagggcg ggcatggagc agagcctcat aattgtatag acccaattgc agttggaaat
     7021 gcagtatatt catccattca acagatagtt tcaagaaaaa ttgacccagt tgtacctgta
     7081 gtagtgtctg tttgttcttt taattctggt gtttctaaaa atataatacc agatgtatgc
     7141 acacttcaag ggactattag agcgataagc aaggaacaag taattgaagt aagtaaaatt
     7201 ctagaaaata tagtaagaaa tgtatgtaga gcatacggag cagattgcaa atttgaaaag
     7261 agtacaggag gagatgctgt tataaatgac aaaaacatga tagagctagg aagagaaagt
     7321 gcttgcaaaa tacttggagt tgaaaatgtt gaaataattg attttcctgc aatgacaggt
     7381 gaggattttg ctatttatat gaaagaaaaa cctggtttat ttatgtatat aggtgtagga
     7441 aataaagaaa aaaatataaa ctacagattg catagtaaca agtttaatat agatgaaaaa
     7501 tgtcttagta tagcatcttc attattctca caacttgcag tagattattt atgtcaacta
     7561 tagagtacat tcataaaaat atatttagcc tatttttaaa tttaatgcta gtatattaat
     7621 tttgatgtag aacatattat ttattatatt tatttaaaaa ggtggataac aatgataaaa
     7681 gtagaaaatt tacataaaag ttttaaaggg acagaagttc ttaaaggtat aaatttagag
     7741 ataaatcaaa gtgaagtagt agctatactt ggaccatctg gaacaggtaa atctacgtta
     7801 cttagatgtt taaattattt agttgagcct acaaaaggaa agataatgat tggtgatgta
     7861 aaagtagatg tagaaaaagc aacaaaaaaa gatatagcca atttaagaaa acatacatct
     7921 atggtatttc aaaattataa cttatttaaa aataaaacag cattagaaaa tgtaatggag
     7981 gcattgatag tagtacataa aaagagtaat aaagaagcta gagaaatatc tttaaaatta
     8041 atagataaag taggtatgtt ggataggaaa gatttttatc catcaaaatt gtctgggggg
     8101 cagcaacaaa ggataggtat agcaagagcg atggcagtaa atccaaatgt aatattattt
     8161 gatgagccaa catctgcact tgacccagaa cttgttggag aagttttaag cgttataaaa
     8221 tcgcttgcga aagaaggaat aacaatgctt attgtaactc acgaaataaa ttttgcaaaa
     8281 gaagttgctg ataaaattat atttatggat aatggtgtaa tagctgaaga aggaactcct
     8341 gatgaaatat ttaataatcc taaaaatgaa agaactgcaa aatttttaaa tatcgttagg
     8401 aaagaagaag tatttagtta aaagtgtata aattaagtat tagaagatat gtaagatact
     8461 cccattaaa
//
LOCUS       DAMJWP010000154         8442 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.159, whole genome shotgun sequence.
ACCESSION   DAMJWP010000154 DAMJWP010000000
VERSION     DAMJWP010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8442
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.159"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            26..1573
                     /locus_tag="PC700_002714"
     CDS             26..1573
                     /locus_tag="PC700_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2582011.1"
                     /translation="MNKNSMTKIYSLNKIIFIIIGILGIVLLTRHIEVDIKVLILILL
                     TLFSINVFISYLKIKLYEDKINLGLINNSDKFINITKDENFLKHVQNYIISNEKENCV
                     MACFDLCRLKLINDIYGYELGDEVLNNILSNLKNYFGKEAIYGKLKSDVFVLIIKLEN
                     REQKIPYLVNLIKNKIMILSQIDSFKMDINIEASIGIYKFNNNEIDIKKAIDNADMAR
                     LKSKGLKHIEYVEFDNAMEEEIQSIMKIERDLFLAIKNKEFVIHYQPKVDSSTGNIIG
                     SEALIRWLHPSLGMVGPNKFIPIAEKNGLINNIGRWLIQEVFITINKWISEGINVLPV
                     SINLSRVELYKNDLVDFFKLMFNTYNIPKELIELEITETTALRDVKFISERLYEIKSL
                     GMNISLDDFGVGNSNFINLKGIPLDIIKIDRSLILDIVTNTKTKFMVKAIINLSHDLN
                     VTVICEGVEDMHQVKVLSELGCNFIQGYVFFKPLDEMNYKKLLTDGSYINLKDTLLDK
                     CMTVEEE"
     gene            1576..1974
                     /locus_tag="PC700_002715"
     CDS             1576..1974
                     /locus_tag="PC700_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDF2582012.1"
                     /translation="MISEDVDYSIRIIRAIFNNGEDKPIDAKMILKSEDIPILTLYRL
                     LRKLCANNILTFNKEDGKTYMLNVSLDTISLRTIVEVIDGKIYINKCTRYVSNCSNCL
                     KGCKVNRELLEIEKEIYKLLSRKSIRQILE"
     gene            2014..3747
                     /locus_tag="PC700_002716"
     CDS             2014..3747
                     /locus_tag="PC700_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HDF2582013.1"
                     /translation="MDDISQDNFLLSKEYENSLDVDTKKASGIYYTPKIIVDYIVKKT
                     LKNHDIIKNPYPRILDISCGCGNFLLEVYDILYDLFEENIYELKKKYDENYWTVDNIH
                     RHILNYCIYGADIDEKAISILKDSLTNKKVVNDLDESDIKINLFCCDSLKKKWRYKFD
                     YIVGNPPYIGHKKLEKKYKKFLLEKYSEVYKDKADLYFCFYKKIIDILKQGGVGSVIT
                     PRYFLESLSGKDLREYIKSNVNVQEIVDFLGANIFKNIGVSSCILTFDKKKTKETYID
                     VFKIKNEDICINKFETLEELLKSSKFEHFNINQRLLSDEWILVNKDDETFYNKIQEKC
                     KYSLEDIAISFQGIITGCDKAFILSKDDVKLNLVDDKFLKCWIKSKNINKYIVDKSEY
                     RLIYSNDIDNENTNKKILDEIIGLYKTKLENRRECKSGIRKWYELQWGREKLFFERKK
                     IMYPYKSNENRFAIDYDNNFSSADVYSFFIKEEYLDKFSYEYLVGILNSSVYDKYFKI
                     TAKKMSKNIYDYYPNKVMKIRIFRDNNYEEIENLSKQIISILLNKSIDKGKVEKLQIK
                     MDNLIMDSLGI"
     gene            3979..5190
                     /locus_tag="PC700_002717"
     CDS             3979..5190
                     /locus_tag="PC700_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDF2582014.1"
                     /translation="MKGLVLEGGGTKGAYQIGAYKALRELGIEFQGVTGTSIGALNGA
                     YIIQNDIEVMEDIWLNYDYTHFMNIDRETYERFKNVDFTTKNLNAVFELINKARKNEG
                     IDISPLRQLMETTLNEDIIRKSKKDFGLVTVWWDKKINPYPLYIEDIPKGRLIDYLIA
                     SSSLPIFQTNMMDDKLYLDGMFCDNMPIGLLEDKGYEDVVVIRLIDDFIGKMNLNRHQ
                     NINIKTIIPSEYLGGSLNKDRDNVEKNIRLGYLDTMKAYERCTGIKYYFDVEYKYNEE
                     YCFDKFRSMSNETIQYICNLLNIKRDASLRTVMESVIPKLGEVLGLPKDFSYKDLFYC
                     IYEKKLEENNINRIKLYDFNKVIDVVNTNIDSNRNLASNFEVRPKISIKKNKNTKLLT
                     NCIIKDIKNQS"
     gene            5382..5642
                     /locus_tag="PC700_002718"
     CDS             5382..5642
                     /locus_tag="PC700_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HDF2582015.1"
                     /translation="MEKVVSIKNASGLHARPAGMFVKKAAEFKSTVEVVAKDKTVNAK
                     SIMGIMSLGLAQGEELKLVVNGEDEEVALAAMVELVESGFGE"
     gene            5995..7707
                     /gene="ptsP"
                     /locus_tag="PC700_002719"
     CDS             5995..7707
                     /gene="ptsP"
                     /locus_tag="PC700_002719"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDF2582016.1"
                     /translation="MAYKGIGASPGVALGKALVVEHSELVIEKKSIDNVEAEIAKLED
                     AVAVSKEELVKVKEKASEELGAEEAEIFEAHLLVLEDPELIGSAIDKIKTESVNAEYA
                     LNEIKEMFVSMFESMDNEYMKERAADIKDVTNRILRHILGIKVVDLSALSEEVVLIAH
                     DLTPSDTATMNKKMVLGFLTDIGGRTSHTAIMSRTLEIAAIVGLNDITSKVKDGDFVV
                     FNGDTGEVIVNPDEETINKYTELKAKYEDERKALQLLKGKPSVTLDGKHVELAGNIGT
                     PNDIEGLIKNDAEGVGLYRTEFLYMDRDSFPTEEIQYEAYKAVLEGMDGKPIVIRTLD
                     IGGDKELSYLSMEPEMNPFLGYRAIRLCLDRKDIFKTQLRALYRASIHGRLRIMFPMI
                     SSLEELLQAKEVVKEVLAELDSEGVAYAKDVEIGMMIEVPSAAVISDVLAKHVDFFSI
                     GTNDLIQYTCAVDRMNQKISYLYNQFNPAVLRLIKTVIDNAHKEGKWAGMCGESAGDQ
                     KMIPILLGMGLDEFSMSPISILPARKLITSVKESDMKKLADDVLNMGTAEEIKSYIEK
                     TFNI"
BASE COUNT         3512 a          736 c         1447 g         2747 t
ORIGIN      
        1 taatttaaaa aataggggaa tttatatgaa taaaaatagc atgacaaaaa tttacagttt
       61 aaataaaata atttttatta taattggaat tttaggaata gttttactta caagacatat
      121 tgaggtagat attaaagttt taattttaat tttattaact ttgtttagta taaatgtatt
      181 tatttcatat cttaaaatta agttgtatga ggataaaatt aatttaggat tgataaataa
      241 ttcagataag tttataaata ttactaaaga tgagaacttc ctaaaacatg ttcaaaatta
      301 tattatcagt aatgaaaaag aaaactgtgt aatggcttgt tttgatttat gtagactcaa
      361 acttataaat gatatatatg gttatgagct aggggatgaa gtgttaaata atattttaag
      421 taacttaaaa aattattttg gaaaagaagc tatttatgga aagttaaaaa gtgatgtttt
      481 tgtattaata ataaagcttg aaaataggga acagaagatt ccttatttag taaatcttat
      541 caagaataaa attatgatat taagtcagat tgacagtttt aaaatggata taaatattga
      601 ggcttctatt ggaatttata aatttaacaa taatgaaatt gatataaaaa aagctataga
      661 taatgctgat atggcaaggc ttaaatcaaa aggactaaag cacatagagt atgtagaatt
      721 tgataatgct atggaagaag aaatacaatc aattatgaaa atagagagag atttattctt
      781 ggctattaaa aataaagagt ttgtaattca ctatcaacct aaagttgatt caagtacagg
      841 taacattata ggttcagagg cattgataag atggttgcac ccatcattag gcatggttgg
      901 accaaataag tttataccta tagctgaaaa aaatggtctg ataaacaata ttggtagatg
      961 gctaattcaa gaagttttta ttaccattaa caagtggatt tcagaaggta taaatgtctt
     1021 accagtctca ataaatcttt ctagagtaga actatataaa aatgatttag ttgatttttt
     1081 taaactgatg tttaatacat ataatattcc aaaagaactt atagaattag aaattacaga
     1141 aacgacagcg ctaagagatg taaaatttat aagtgaaagg ctttatgaga ttaaatcttt
     1201 aggtatgaat atatcattag atgattttgg agtaggtaat tcaaatttta tcaatcttaa
     1261 ggggatacca ctagatatta taaaaattga taggtcactt atattggata tagttacaaa
     1321 tacaaaaaca aagtttatgg tgaaagcaat tattaacctt tcacatgatt taaacgtaac
     1381 tgttatatgt gaaggggtag aggatatgca tcaggtcaaa gtcttaagtg aactgggatg
     1441 taatttcata caaggttatg tattttttaa acctttggat gagatgaatt ataaaaaact
     1501 tcttactgac ggaagttata taaatctgaa agatactctt ttagataaat gcatgacagt
     1561 tgaggaggag taaggatgat ttcagaagat gtagattatt caataagaat cataagagct
     1621 atatttaata atggagaaga taaaccaata gatgctaaaa tgatattaaa aagtgaagat
     1681 ataccaatac taacactata cagactgctt agaaagcttt gtgcaaacaa tattcttact
     1741 tttaacaaag aagatggcaa gacatacatg ttaaatgtgt cattggatac aataagtcta
     1801 agaactattg tagaagtaat tgatggaaaa atatatataa ataaatgtac gagatatgta
     1861 tctaattgca gtaattgctt aaaaggttgt aaagtaaatc gtgaattgct agaaatagaa
     1921 aaagaaattt ataaactttt atcgagaaaa tctataagac aaatactaga ataaaaaatt
     1981 ttcagctatg ataataattg gaggttttcg gttatggatg atatatctca agataatttt
     2041 ctactgtcta aagagtatga gaatagctta gatgtagata ctaaaaaagc aagtggaata
     2101 tattatactc ctaaaataat tgtagattat attgtaaaaa aaactttaaa aaatcacgat
     2161 ataattaaaa atccatatcc acgaatactt gatatttcct gtgggtgtgg aaattttctt
     2221 ttagaagtat atgatatttt atatgactta tttgaggaaa atatatatga attgaaaaag
     2281 aagtatgatg aaaattattg gactgttgat aacatacacc gtcatatatt aaactattgt
     2341 atatatggtg ctgatattga tgaaaaagct ataagtatat taaaagatag cttaacaaat
     2401 aaaaaggtag taaatgattt agatgaatct gatataaaga ttaatctatt ttgttgtgat
     2461 agtcttaaaa agaaatggcg atataagttt gattacatag tgggtaatcc accctatatt
     2521 ggtcataaaa aacttgaaaa gaaatataaa aaatttctcc tagaaaaata tagtgaagtt
     2581 tataaggata aagctgactt atatttttgt ttttataaaa aaattataga tattttaaag
     2641 caaggagggg ttggcagtgt tataactcca agatatttct tagaaagtct atcaggtaaa
     2701 gatttaagag aatatattaa atcaaatgtc aatgttcaag aaatagtgga ttttttaggt
     2761 gcaaatatat ttaaaaacat aggggtatct agttgtattc taacttttga taagaaaaaa
     2821 acaaaagaga catatataga tgtatttaaa atcaaaaatg aggatatatg tataaataaa
     2881 tttgaaacat tggaagaact tctaaagagt agcaaatttg aacattttaa tataaatcag
     2941 agattgctat ctgacgaatg gatacttgta aataaagatg atgaaacttt ttataataag
     3001 atacaagaaa aatgtaaata tagcttggaa gatatagcaa taagttttca aggaataatt
     3061 acagggtgtg ataaagcgtt tattttaagt aaagatgatg taaagttgaa ccttgtagat
     3121 gataaatttt taaaatgttg gataaaaagt aagaatataa ataaatatat agtagataaa
     3181 agtgaatata ggctaatata ctcaaatgat atagataatg agaatacaaa taaaaagata
     3241 ctagatgaaa ttatagggct atataaaaca aaattagaaa acagaagaga atgtaaaagt
     3301 ggaataagga aatggtatga acttcaatgg ggtagagaaa agcttttctt tgaaagaaaa
     3361 aagataatgt atccttataa atcaaatgaa aatagatttg ctatagatta tgataacaat
     3421 ttttcaagtg cagatgtata ttcttttttt ataaaagaag agtatttaga taaattttca
     3481 tatgaatatc tagttggaat tttaaattcc agtgtttatg ataaatactt taaaataact
     3541 gctaaaaaaa tgagtaaaaa tatatatgat tactatccta ataaggttat gaagataagg
     3601 atttttaggg ataataatta tgaagagata gagaatttat caaagcaaat aatatcaatt
     3661 ttattaaata agtctataga taaaggaaaa gtagaaaaat tgcagataaa aatggataac
     3721 ttgataatgg attctttagg tatataagca gttgtaagta ttaaaaaatt ataatccttt
     3781 aattgtttat ttaaatttag aaataaaaag ttagccatat aaaaaatatt aaaaatatat
     3841 acttttcaat atttttttga taaaataaga atgaatttgt gcaattttat ttttctccaa
     3901 taactaaaaa tagttttttg aattaatatt tgcttaagaa taaaaatcag aaacgttttt
     3961 gatatagtag gtgataatat gaaaggatta gttttagaag gtggaggaac aaaaggagcc
     4021 taccaaatag gtgcatataa agctttaaga gagcttggta tagagtttca aggagtaact
     4081 ggcacatcaa ttggtgcttt aaatggagca tatataattc aaaatgatat tgaagtaatg
     4141 gaagatattt ggttaaatta tgactatact cattttatga atattgatag agaaacctat
     4201 gagaggttta aaaatgtaga tttcactact aaaaatctca atgcagtgtt tgagcttata
     4261 aataaagcta gaaaaaatga aggtatagat ataagcccat taagacaact aatggagaca
     4321 acattaaatg aagacataat aaggaaatca aaaaaagact ttggacttgt tacggtctgg
     4381 tgggataaaa aaattaatcc atatccattg tatatcgaag atatacctaa aggaagattg
     4441 attgattatt taattgctag ttccagtttg cctatttttc aaacgaacat gatggatgat
     4501 aagttgtatc ttgatggaat gttttgtgat aacatgccaa taggactctt ggaagacaaa
     4561 ggttatgaag atgttgttgt aattaggttg attgatgatt ttattgggaa aatgaatcta
     4621 aatcgacatc aaaatataaa tataaaaact ataataccat cagaatatct aggaggttct
     4681 cttaataaag atagagataa tgtagaaaaa aacatccgac taggttactt agatactatg
     4741 aaagcatatg aaagatgcac tggaattaag tattattttg atgtagagta taaatataat
     4801 gaagaatatt gttttgataa atttagaagt atgagcaatg aaacaataca atatatctgt
     4861 aatttattga atataaaaag agatgcttca cttagaactg ttatggaaag tgtaatacca
     4921 aagcttggtg aggtgcttgg attgccaaag gacttttcat ataaagattt gttttactgt
     4981 atatatgaaa agaaacttga agaaaataat ataaacagga taaaattata tgattttaac
     5041 aaggttatag atgttgtaaa tacaaatatt gattctaata gaaatttagc ttctaatttt
     5101 gaagttagac ctaaaattag tattaaaaag aacaaaaaca caaaattatt gacaaattgt
     5161 attataaaag acataaaaaa tcaaagttaa ataaaaaaca aaaaaaaatt tgtaattata
     5221 ctcgaaaaaa gtgtatatta gtagtataat atttttgtag cactagtggg aacaaaaaat
     5281 gaccatagga caaaaaagta ccacttagta ccattaaagt aaaaaacagg aaaacaattt
     5341 taaaaaatat aaaatgtaaa gtttatagga ggatttaaaa aatggaaaaa gtagttagta
     5401 tcaaaaatgc aagtggatta catgctagac cagcaggaat gtttgttaag aaggcagctg
     5461 aatttaaatc aacagtagag gttgtagcaa aagacaaaac tgtaaatgca aaatcaataa
     5521 tgggaataat gagtttaggt ttagctcaag gagaagaatt aaagttagtt gtaaatggag
     5581 aagatgaaga agtagcttta gctgcaatgg ttgaattagt tgaaagtgga tttggagaat
     5641 agatataata attttttaaa atttgatatt tagaatctaa tattacttaa agtaaagtaa
     5701 cagagaagta ttaaacatat acagaccaac atttgtagat atttaatttc tttgttactt
     5761 tcatgtttat ttaatcagaa gattaagact attgttaatt gggtataaaa atataaatat
     5821 tttagaaaat gaaataagat atttttaatc ataaattcag agtaaaaata cagtaacaaa
     5881 cagtttataa aatttaataa aaaattataa gtaaataaaa ctataaaaat gtaaattatt
     5941 tatataaatt tatagagttt atgtgctaga tgatattttt taggaggtag ggttatggct
     6001 tacaaaggaa taggtgcttc tcctggtgtt gctttaggga aagcgttagt agttgaacat
     6061 agtgaattag taattgaaaa aaaatctata gataatgttg aagctgaaat tgctaaatta
     6121 gaagatgcag ttgcagtttc taaagaagaa ttagtaaaag ttaaggaaaa ggcttctgaa
     6181 gaattaggag cagaggaagc agaaatattt gaagcacatt tattagtttt agaagaccca
     6241 gagttaattg gttctgcaat agataaaatt aaaactgaaa gtgtaaatgc agagtatgct
     6301 ttaaatgaaa taaaagagat gtttgttagc atgtttgagt ctatggacaa tgagtacatg
     6361 aaagaaagag cggcagatat aaaagatgtt acaaatagaa tacttagaca tatattagga
     6421 attaaagttg ttgatttatc tgctttaagc gaagaagttg tattaatagc acatgattta
     6481 actccatcag acacagcaac tatgaataaa aaaatggttt taggattttt aactgatata
     6541 ggtggaagaa cttctcatac tgctatcatg tctagaactt tagaaatagc tgcaatcgtt
     6601 ggattaaatg atataacttc aaaagttaag gatggagatt ttgtagtttt caatggagac
     6661 acaggtgaag taattgtgaa tcctgatgaa gagactataa ataaatatac tgaattaaaa
     6721 gctaagtatg aagatgagag aaaagcatta cagctattaa aaggaaaacc atctgtaact
     6781 ttagatggaa aacatgtgga attagctggt aacataggaa cacctaacga tatagaaggt
     6841 ctaataaaga atgatgctga aggtgttggt ctttatagaa cagaattttt atacatggat
     6901 agagattctt tcccaacaga agaaatacaa tatgaagcat acaaagctgt attagaagga
     6961 atggatggaa agccgatagt tataagaaca ttggatatag gtggagataa agaattaagt
     7021 tatttaagta tggaacctga aatgaatcca ttcttaggtt acagagcaat aagactttgc
     7081 ctagatagaa aagatatatt taagactcaa ttgagagctt tatatagagc tagtattcat
     7141 ggaagattaa gaatcatgtt cccaatgatt tcatctttag aagaattgtt acaagctaaa
     7201 gaagttgtaa aagaagtttt agcagaactt gattctgaag gtgtggctta tgctaaagat
     7261 gtagaaattg gtatgatgat agaagttcca tctgctgctg taatatctga tgtattggct
     7321 aaacacgtag atttcttctc tataggaact aatgatttaa ttcaatacac ttgtgcagtt
     7381 gatagaatga atcaaaagat aagttatctt tacaatcaat ttaatccagc tgttttaaga
     7441 cttataaaga ctgttataga caatgcacat aaagaaggaa aatgggcagg tatgtgtgga
     7501 gaatctgccg gagaccaaaa aatgatacca atacttttag gtatgggatt agacgagttc
     7561 tctatgtctc caatatcaat acttcctgct agaaagttaa taacttcagt taaagagtca
     7621 gatatgaaaa aattagctga tgatgtactt aatatgggta ctgctgaaga aataaaaagc
     7681 tatatagaaa agacttttaa tatataatta atgttatata gttactaaaa ttcatttttc
     7741 taaataacaa aattatattt tacgaattaa aaaggagatg tttcaaaata aaaatttgaa
     7801 gcatctcttt tttataaaag attactaaga attacttaat ctttgtaata gtcattttgt
     7861 gaaaaagcaa aactgcattg tgactatagt atatatggat gttattatca ataaaagtac
     7921 tattaaaatg aagattcatt ctcaaatgat atcaaaagta gttgtgatgg agtaagataa
     7981 tattttgtga tttaatagta aaaattaaaa aaatatggta ttgaaaaata attaaaagta
     8041 tttaataata gaattaggga agaataagtt tataagtaaa aataattgat tactagttaa
     8101 taaaataaat gtgatatcca aataaatcct aaatctaatt tgagttttaa tatagatagt
     8161 ctaaagtttg attattaaag atttaattgt aagttgttac agttgatgat attgattaga
     8221 gattaaagtt tagagtagaa agttacctta aatttaaaaa tgcaagataa cccaatctaa
     8281 aaattataat cactcgatag gtttaaggat ttatttgttt attataaata ggagggtgtc
     8341 tcaaaatgaa ctttttagtt tatgaggcac tctcttttat atagaattaa atatatttta
     8401 ttatttcaac aatgaaaaag aagcttatct ctattttgag ac
//
LOCUS       DAMJWP010000155         8435 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.160, whole genome shotgun sequence.
ACCESSION   DAMJWP010000155 DAMJWP010000000
VERSION     DAMJWP010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8435
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.160"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     regulatory      66..151
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            275..6238
                     /locus_tag="PC700_002720"
     CDS             275..6238
                     /locus_tag="PC700_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HDF2582017.1"
                     /translation="MKKAISCVLAVSMCSAPLNVFAEPILEGKLRAVEKSVTEKLRGN
                     LEVDLNFSLPIKNSESESMTNMQLRLKDDSNNSGIIKLGEKDSGTVNIGTDNIEYTIK
                     KLSASRSEIKNKDENVSYYNIVFNNLPVGKYNIEVSGAGFKNKEIKDIDISSYSQRVL
                     LSNRASVDNKNKPLNYELFLMGDVNGDGKVDKSDYNKVLENIDSNKREFDLNRDGKVD
                     IVDLDYVQKNLGQSEDAKSLESIVSTNPIVDTSKVELKGNSDVDINGNVENLFSGENN
                     GVTISSKNDEISEEKPAVMDIALSEALNMEVINIKAPEGTAPAKGIVEVTDEDGNVQQ
                     IPYENKEYMKNLKDKEQQEEVNNEQEEVNQEEVNNKDENKNQTDNNSNNKDENNDKQQ
                     ENSDKQEVNSNTNQDKNNDKENSSMDKPNSDEDTPKEGDNISKDDNSTTSTYSRAALE
                     TYTDVQNSDIVINLGKQVAVKKITIKITATTANDRNLAEISKVEFLNNVYKEIPKPEM
                     NIPKINKISTSTAVGNEKVSFYWNNEVNVSAYELILDKIDSKGKVLSTKKLQTSKNTI
                     EVRDIEAYGLYRVSIQSLNGDWSSGYKDATPENLDGVPENVDSNYKPIQFSPDSILEF
                     QVVPDTKPEPPEGINVKGKFKSLDVSWKNHKQAKDFDLYYKEKGSNGSWIKYNDKLIR
                     GTSLKIEGLKDNTTYEVRMTATNHLGTSGMSKTYIGTTESIDAPTTPNYRLINTPKEN
                     GGFGIPTNHIVDVVYPAGYDKSEYSDKNPFDKFNVVDGDFTTHWTFPTYNAAEGTNRG
                     PIITFDKSYTMDTFMATPRFDVSDKTINDFEIRYWDENGQLHNLGDVPFETKTDPVNN
                     NKYVMVRLKEPITATKIQVSVCSSYSYKSNISELKFYEYDSIEDDVRNLFMDDLQVEL
                     KEDVTQEKITELKDRLNTPDKASGEYHPYKTVIDRELKLAQDLYNDRATLTDVTTVNQ
                     EINKSGKYKDKNGQYTVNSNNLGMQNDWQALGVAARAGDEITVYVGSKSGKTPKLIYT
                     QYYGESGAYKSGEINLKTGKNVIQLSKLHSLDIECGGSLYIRYPEDTPSGGDIKVRVA
                     GATKIPHLNLNGTINDKSPAGVSESKKKIKAYIEELAKYKDAVKGEPYYPGTNIGNSY
                     EYSEKTGVLNTTDIESDKVTLNVPATAVYQGISKGNADLNTQVDRLYNSMLAWEQIMD
                     LVYSERGVFKTQDLDGNGTVDDNEFNLTKNDMAPKSRMNIKYQRMFIGAFMYASGLHV
                     GVEYGSVPGLMNGVPFQIDDSGKATGGNLFGWGIGHEIGHVTDIGKMTYSETSNNVLA
                     LLAQTFDDKTHSRLEGSTMDKIYEHVTSNSLGIPSNVFERLGMLWQLHLAYDDDFTGS
                     MLKNNSDADLSNDTFYAKISRKYRALSSSDPINSLPKDQMLVAMASSVVEKDLRDYFK
                     AWGVEITPELNSIMDSKNYEKESRKIQYVNDEARRKILNENIASMAKDTKVSASFSDG
                     IQSGSLVKSNKISIDLDVDKDKDKILGYEIIRSDGNYLDGENGTQVKYRTVGFVDAKG
                     NSKAKFTDSISPLNNRAFTYKVVAYDYHLNPTEEFEVGTVKLSDEGKINKSAWSFITN
                     TVSDGDVRTENDSHGPLQNPEIDNIKDNDVSTTYKGKIISKAEWNKDPQKDPDINVEE
                     NPYIIVDMKETLPIVGLKYTKPEAETRKFSLKGLFNFRKNTSTTYNPLTNYKIQVSND
                     KKNWEDVSQGTFEYGQDLVGGGKDQDNEARVTFNKDDKLWTYQARYVKLISNNKSNIE
                     VAELDIIGPPGDNIEIGTMNNADQTRTNGIGKLAEDYVYQSDDPSTSEDETGKIPAGS
                     IIITGEYSGNPAFNLPLLIDKNNKTISGEALLFAEVPEKGELGEISKGTWVYYMPKEN
                     FDSLSDKVKAELYRYNDLQGDTPVGQRFVSDTMYVPVGAKNYDELKTISLTDSNSKAR
                     KATRNAIDVSNKKVITVSNKVKGNIVRDK"
     gene            6448..8328
                     /locus_tag="PC700_002721"
     CDS             6448..8328
                     /locus_tag="PC700_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp10"
                     /protein_id="HDF2582018.1"
                     /translation="MSKRKSFIRTIAVSTMAVAVTGSVTCAYAAPVLQGTKTYEKVNT
                     IDISVDSVENIVYSFQASIKVQGEVEVVDNEQKEKITWSDNIKSQIKSGNANATCRVE
                     YNKSSNTTTLDIYVTSNEDLLDGNRLNIGRISVKKSGSNSNADYKVLGKGTSDKPALK
                     IVTYNNKTVDYENINSDEGLIFTLINESEVKPIGGTGSSKNDPEKYKVEKSEALEYLL
                     NNIRINYSIVSKETQESGSNVILKLGLAQKITKGRKATINKYVEVTLPKSLEYIVENE
                     LSKPDELPPDNGNGGNNGGGSNSGGSSSGGSSGGGSSSNSTSNVTVKKLKGADRFETA
                     TKISQSGWTKSDTVVIVNGEDKSMVDGLTATPLASVKNSPILLSSNEKLPQKTVEELK
                     RLNPSKVIVIGGNNSMPNSVVEAIKAVNSKISVQRIGGDTRYQTSINIAKEIDKTNNV
                     SKLYIGAGNGEADSLSIASLAGKEKTPIVLTQKDGVDNEAVQFIKSNKVSNIYFIGGI
                     EKISNKAIEQVGKIANKDISNNRVAGQTRQETNAKVIDKFYSQSKLDGVVVANQDKLI
                     DALAVGPLAAKNNSPVILATNTLDKSQESSLKGKNSSKLFEVGGGIASSVVDKIKSLI
                     EK"
BASE COUNT         3600 a          972 c         1568 g         2295 t
ORIGIN      
        1 aatttaatgt aaaaaaattg tattaaaaag tttaaatata gtataaaatg ggtaagaaag
       61 tagtgaaaat taaatggcaa aattagggaa atctaatgac gcaaaactat agggattaaa
      121 gcttttaaag ccatatcagc cagttgctaa aaagagtaaa gtctttttat tttataatta
      181 agattctctt tgagtgtctt tttttattgt ctgaaactta tagtatttaa attcatgaaa
      241 aattagagat ttattaaggg aggattgttg tttcatgaaa aaggcaatat cttgtgtact
      301 agcagtatct atgtgtagtg caccactaaa tgtttttgca gaacctatat tagaggggaa
      361 gctaagggca gttgaaaaat cagttacaga aaagttaagg gggaatcttg aagtagattt
      421 aaatttttca ctgccaataa aaaatagcga gtcagaaagt atgactaata tgcagttacg
      481 attaaaagat gatagtaaca atagtggaat aataaagctt ggagaaaaag attcaggaac
      541 agtaaatatt gggacagata atatagaata tactataaaa aaattatctg caagtagaag
      601 tgaaataaaa aataaagatg agaatgtatc atattataat attgtattta ataatttacc
      661 tgttggaaaa tacaatatag aagtctctgg agctggattt aagaacaaag aaattaaaga
      721 cattgatata tcaagttatt cacaaagagt cttattaagc aatagagcaa gtgtagataa
      781 taaaaataaa cctttaaatt atgaattatt cttaatgggt gatgtaaatg gagacggtaa
      841 agtagataag agtgattata ataaggtttt agagaatata gattcaaata aaagagaatt
      901 tgatttaaat agagatggaa aggtagatat tgttgactta gattatgtac agaaaaattt
      961 aggacaaagt gaagatgcaa aaagcctaga aagtatagta tctaccaatc ctatagtaga
     1021 tacaagtaag gttgaattaa aaggaaatag tgatgtagat attaatggta atgtagaaaa
     1081 tttatttagt ggagaaaata atggagttac aatatcttca aaaaatgatg aaatatctga
     1141 ggaaaaacca gcagtaatgg atatagcttt atcagaagca cttaatatgg aagtcatcaa
     1201 tataaaagct ccagaaggta ctgctccagc aaaaggaata gttgaggtta ctgacgaaga
     1261 tggtaatgtt caacagattc cttatgaaaa taaagaatat atgaaaaact taaaagataa
     1321 ggagcaacaa gaggaagtaa ataacgaaca agaagaagta aatcaagaag aagtaaataa
     1381 taaagatgaa aataaaaatc agactgataa taattcaaat aataaagatg aaaataatga
     1441 taaacaacag gaaaatagcg ataaacaaga agtaaactct aataccaatc aagataaaaa
     1501 taatgataaa gaaaattcat caatggataa acctaattca gatgaagata ctccaaaaga
     1561 aggggataat atcagtaaag atgataactc tacaacatct acatatagta gggcagcatt
     1621 agaaacatat acagatgttc aaaatagcga tatagtaata aatcttggta aacaagtagc
     1681 agtaaaaaag ataactataa aaataacagc tacaacagca aacgatagaa atcttgcaga
     1741 aatatcaaaa gttgaatttt taaacaatgt atataaagaa ataccaaagc cagagatgaa
     1801 catacctaaa ataaataaaa tatcgactag cactgctgtt ggaaatgaaa aagtatcttt
     1861 ttattggaat aatgaagtaa atgtatcagc atacgaactt atactagata aaattgatag
     1921 taagggaaaa gtattatcta ctaaaaaatt acaaactagt aaaaatacaa tagaagttag
     1981 agatatagaa gcatatggac tatatagggt tagcatacaa tctttaaatg gagattggtc
     2041 tagtggttat aaagatgcaa cgccagaaaa cttagatgga gtaccagaaa atgtagactc
     2101 aaactataaa cctatacaat ttagcccaga tagcatatta gaatttcaag ttgttccaga
     2161 cacaaaacca gaaccaccag aaggtataaa tgttaaaggt aaatttaaat ctcttgatgt
     2221 aagctggaaa aatcataaac aagctaaaga ctttgacttg tactataaag aaaaaggctc
     2281 taatgggtct tggataaaat acaatgataa gcttattcgt gggacaagtt taaaaataga
     2341 aggtcttaag gataacacaa catatgaagt tagaatgaca gctactaacc acttgggaac
     2401 tagtggaatg tctaaaacat atataggtac aactgaaagc atagatgcac caacaacacc
     2461 taactataga ttaattaata ctcctaagga gaatggtgga tttggaatac caacaaatca
     2521 cattgtagat gttgtttatc cagcaggata tgacaaaagt gaatatagtg ataaaaatcc
     2581 atttgataaa ttcaatgtag tagatggaga cttcacaaca cattggacat tcccaacata
     2641 taatgcagct gagggaacga atagaggacc aataataact tttgataaat cttatactat
     2701 ggatactttt atggctacac ctcgttttga tgtttctgat aagacaataa atgactttga
     2761 gatacgatat tgggatgaaa atggtcaatt acacaatctg ggggatgttc cttttgaaac
     2821 aaagacagac cctgtaaata ataataagta tgtaatggtt agattaaaag aaccaataac
     2881 tgctactaag atacaagtaa gtgtttgttc atcttattca tataagtcta atatatcaga
     2941 acttaagttt tatgagtatg acagtataga agatgatgta agaaatctat ttatggatga
     3001 cctacaagtt gaattaaaag aagatgtaac tcaagaaaaa ataactgaat taaaagatag
     3061 attaaatact ccagataaag ctagtggaga atatcatcca tataagacag ttattgatag
     3121 agaattaaaa ttagctcaag acttatataa tgatagagcc acacttactg atgtaactac
     3181 tgtaaatcaa gaaataaata aatcaggaaa atataaagat aaaaacggac agtatactgt
     3241 aaattccaat aacttaggca tgcaaaatga ctggcaggca ttaggagtag ctgctagagc
     3301 aggagatgaa ataacagtat atgttggaag taaaagtgga aaaactccta agttgatata
     3361 tacacagtac tatggagaat ctggtgcata taagagtgga gaaataaatc ttaaaactgg
     3421 aaaaaatgta attcaattaa gcaaacttca tagtttagat atagaatgtg gaggaagttt
     3481 atacataaga taccctgaag atactccaag tggaggagat ataaaagtac gtgtagcagg
     3541 agctactaag ataccacatc ttaatctaaa tggaacaata aatgataaga gtcctgcggg
     3601 agtttctgaa tctaagaaaa agataaaagc atatatagaa gaattagcta aatataaaga
     3661 tgctgtaaaa ggagaaccat attatccagg aacaaatatt ggtaattcat atgaatattc
     3721 agaaaaaact ggtgtgttaa acactacaga tattgaaagt gataaagtaa cattaaatgt
     3781 tcctgcaaca gctgtatatc aaggaatatc aaaaggaaat gctgatttaa atacacaagt
     3841 agatagatta tataatagta tgcttgcatg ggaacaaata atggatttag tttactcaga
     3901 aagaggagta tttaaaacac aagatttaga tggtaatgga actgtagatg ataatgaatt
     3961 taacttaact aaaaatgata tggctccaaa atctcgtatg aacataaaat accaaagaat
     4021 gtttattgga gcatttatgt atgcttctgg attacatgta ggagtagaat atggttcagt
     4081 tccagggctt atgaatggag tacctttcca aatagatgat agtggaaaag ctacaggagg
     4141 aaacttattt ggatggggaa taggtcatga aataggacat gttacagata ttggaaagat
     4201 gacatatagc gaaactagta acaatgtact tgctttactt gctcagacct ttgatgataa
     4261 gacacattca agacttgaag gttcaacaat ggataagata tatgagcatg taacttcaaa
     4321 tagcttaggt ataccaagca atgtatttga aaggcttgga atgttatggc aattacatct
     4381 agcttatgat gatgatttta cgggttcaat gttaaagaat aactcagatg ctgatttaag
     4441 taatgataca ttctatgcaa agatttcaag aaaatacaga gcactttcaa gtagtgaccc
     4501 aattaatagt ttaccaaaag accaaatgct agtagcaatg gcatcaagtg ttgttgaaaa
     4561 agatttaaga gattacttta aggcatgggg agtagaaatt acaccagaat taaacagtat
     4621 aatggatagt aagaactatg aaaaagaatc aagaaagata caatatgtaa atgacgaagc
     4681 tagaagaaag attttaaatg aaaatatagc tagtatggca aaagatacta aagtaagtgc
     4741 tagtttttct gatggaatac aaagtggttc tctagttaaa tctaataaaa tatctataga
     4801 cctagatgta gataaggata aagataagat actaggatac gaaataataa gaagtgatgg
     4861 aaattattta gatggtgaaa atggaacaca ggtaaaatac agaacagttg gatttgtgga
     4921 tgcaaaagga aatagtaagg ccaaatttac tgatagtata tctcctctta ataacagagc
     4981 atttacatat aaggtagttg catatgatta tcacctaaat ccaacagaag aatttgaagt
     5041 tggcactgta aaattatcag atgaagggaa aataaataaa tcagcatggt cattcataac
     5101 aaatactgtt tctgacggtg atgttagaac tgaaaatgac tcacatggac cattacaaaa
     5161 tcctgaaatt gataatataa aagataatga tgtaagtact acatataaag gaaagataat
     5221 aagtaaagca gagtggaaca aagaccctca aaaagaccct gatataaatg tagaagaaaa
     5281 tccatatata atagtagaca tgaaagaaac attacctata gtaggactaa aatatacaaa
     5341 accagaggca gaaactagga agttctcatt aaaaggatta tttaacttta gaaagaatac
     5401 tagcacaaca tacaatcctt taactaatta caagatacaa gtaagtaatg ataaaaagaa
     5461 ctgggaagat gtaagtcaag gaacttttga atatggacaa gatttagttg gtggaggaaa
     5521 agaccaagat aatgaagcta gagttacatt taataaagat gataaacttt ggacatacca
     5581 agctagatat gtaaaactaa tctcaaacaa taagtctaat atagaagttg cagaattaga
     5641 tataatagga cctccaggtg ataatataga aattggcaca atgaataatg cagaccaaac
     5701 tcgtactaat ggaattggaa agttagcaga agattatgta tatcaaagtg atgaccctag
     5761 cacaagtgaa gatgaaacag gtaagattcc agcaggttca ataattatta caggtgaata
     5821 cagtggaaat ccagcattta atttaccact acttatagat aaaaacaata agactatatc
     5881 tggagaagca cttttatttg cagaagttcc agaaaaaggt gaattaggtg aaatcagtaa
     5941 aggtacatgg gtttactata tgccaaaaga aaactttgat tctttaagtg ataaggttaa
     6001 ggctgaacta tatagataca atgaccttca aggagatacg ccagtaggtc aaagatttgt
     6061 aagtgataca atgtatgtac ctgtaggagc taagaattat gatgagttaa agacaataag
     6121 tcttactgat agtaatagta aagctagaaa agctacaaga aatgctattg atgtcagtaa
     6181 taagaaggtt ataactgtaa gcaataaagt aaagggtaat attgttagag ataaatagta
     6241 ggtggaactc taaaaacgta aaaatactaa tactaacatt gtatatctaa tatgattagg
     6301 tactagaata gttttaaaat agtagatatt aaaagcaaag tatataaatg taactataag
     6361 tttagggtaa tttataaatt aaatataaaa gctaggtgga taagtaaatt tatccactta
     6421 gttaatgata gataaggacg ggtgttaatg agtaaaagaa aatcttttat aagaactata
     6481 gcagtatcaa ctatggcagt agctgtaact ggtagtgtta catgtgcata tgcagctcca
     6541 gttttacaag gaacaaaaac gtatgagaaa gtaaatacta tagatatatc tgtggactca
     6601 gtagaaaaca tagtatattc atttcaagcc agtataaaag tacaaggtga ggttgaagta
     6661 gtagataatg agcagaaaga aaagattact tggtctgata atataaaatc tcagataaaa
     6721 agtggaaatg caaatgctac ttgtagagta gaatacaaca aatcaagtaa tacaactaca
     6781 ttagatatat atgttacatc aaatgaagat ttactagacg gaaatagatt gaatatagga
     6841 agaatatctg taaaaaaatc tggcagtaac tcaaatgctg actataaagt tttaggaaaa
     6901 ggaacaagcg ataaaccagc tttaaaaatt gtaacatata acaacaaaac agtggattat
     6961 gaaaatataa actctgatga aggattaata tttaccctta ttaatgagag tgaagtaaaa
     7021 ccaattggtg gaactggttc tagtaaaaat gaccctgaga agtataaggt agagaaaagt
     7081 gaagcacttg aatatttatt aaacaatatt aggataaact attctattgt tagtaaagaa
     7141 acacaagaaa gtggttcaaa tgtaatatta aaacttggat tagcacaaaa aataactaag
     7201 ggaagaaaag ctacaataaa taaatatgtt gaagttacac ttccaaaaag tttagaatac
     7261 atagttgaaa atgaattatc aaaaccggat gaattaccac cagataatgg caatggtgga
     7321 aataatggtg gtggaagtaa ttcaggcggc tcgtctagtg gtggaagttc tggaggagga
     7381 agttcaagta attctacatc taatgtcaca gtcaaaaaat taaaaggtgc tgatagattt
     7441 gagacagcta ccaaaatatc tcaaagtggt tggacaaaat cagatacagt tgtaatagta
     7501 aatggagaag ataaaagcat ggtggatggg cttacagcta caccacttgc tagtgtaaaa
     7561 aactcaccga ttcttttatc atcaaatgaa aagcttcctc aaaaaactgt agaagaattg
     7621 aaaaggctaa atccatcaaa agtaattgtg ataggtggaa acaactcaat gccaaattca
     7681 gttgttgaag ctatcaaagc tgtaaattca aaaatatcag tacagagaat aggtggagat
     7741 acaagatatc aaacttctat taatatagca aaagaaatag ataagacgaa taatgtaagc
     7801 aaattgtata taggggcagg aaatggagaa gcagattcat tatcaatagc atcactggcc
     7861 ggtaaagaaa aaactccaat agtacttaca caaaaagatg gtgtagataa tgaagctgta
     7921 caatttatta aatcaaataa ggtgtcaaat atatacttta ttggtggaat agaaaaaata
     7981 tctaacaagg ctattgaaca agttggaaaa atagcaaaca aagatatatc aaacaatagg
     8041 gtggcaggtc aaactagaca agagacgaat gccaaagtaa tagataaatt ctactctcaa
     8101 tctaaactag atggagttgt agttgcaaat caagataaac ttatagatgc tttagcagtt
     8161 ggaccattag cagctaaaaa caattcacca gttatacttg ctacaaatac gttagataaa
     8221 tcacaagaat catcattaaa aggtaaaaat tcatcaaagt tgtttgaagt aggtggagga
     8281 atagcctcat ctgtagtaga taagattaag agtttaattg aaaaataaat attaagtaaa
     8341 tcgaaaataa gaatgtattt agatataaag ttatatattt agatgcattc ttattttatt
     8401 ttcattatct taacaattta ataatattga ggtta
//
LOCUS       DAMJWP010000156         8403 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.161, whole genome shotgun sequence.
ACCESSION   DAMJWP010000156 DAMJWP010000000
VERSION     DAMJWP010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8403
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.161"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(186..710)
                     /locus_tag="PC700_002722"
     CDS             complement(186..710)
                     /locus_tag="PC700_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2582019.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQIMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(775..1527)
                     /locus_tag="PC700_002723"
     CDS             complement(775..1527)
                     /locus_tag="PC700_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDF2582020.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDISKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(1558..2856)
                     /locus_tag="PC700_002724"
     CDS             complement(1558..2856)
                     /locus_tag="PC700_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HDF2582021.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVESTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEDIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMEDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQQGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREVNVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(2873..5137)
                     /locus_tag="PC700_002725"
     CDS             complement(2873..5137)
                     /locus_tag="PC700_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HDF2582022.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(5392..6210)
                     /locus_tag="PC700_002726"
     CDS             complement(5392..6210)
                     /locus_tag="PC700_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDF2582023.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGIDEKIIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIIL
                     LDEPTAGLDPVSTRSIVDIIKGLNKDNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFVNENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(6203..6901)
                     /gene="cbiQ"
                     /locus_tag="PC700_002727"
     CDS             complement(6203..6901)
                     /gene="cbiQ"
                     /locus_tag="PC700_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HDF2582024.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIKNIFVLSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEIFSFN
                     VGISKASIDMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKMHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(6904..7188)
                     /locus_tag="PC700_002728"
     CDS             complement(6904..7188)
                     /locus_tag="PC700_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2582025.1"
                     /translation="MSAKTKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEI
                     TKINPDYEPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGVQKGKRS"
     gene            complement(7178..7930)
                     /locus_tag="PC700_002729"
     CDS             complement(7178..7930)
                     /locus_tag="PC700_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HDF2582026.1"
                     /translation="MKQNIKLGVIAALMLIVLTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLVGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIISFLIFKALKKKDG
                     NNAMPVFLAAAIGDLATYTVTSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(8119..8311)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT         2626 a         1535 c          900 g         3342 t
ORIGIN      
        1 atttttttaa taaaacttta ttttttagtt aaaatcatac aatatatagc tttccacaat
       61 acaaataaat taaaaagtga aatgtatttg tctaaaaaca taataacatt tcactttatc
      121 ctcattttta agtaatatat atttttaatt tttatttata actcaatcta gtttttatat
      181 aagttttagt ttttttctac atctgagtaa tctattccaa aagtttctac agtaacagac
      241 ttcataattt gtggagtttg tggcttatca gctgaatctg ttctaactga tgctatttca
      301 tcaactgtat ccataccttc tatcactcta ccaaaacctg catactgacc atctaagtgt
      361 ggtgagtttt tatgcattat gaaaaattga gaaccagctg aattaggtgc catagttcta
      421 gccattgata tgactcctct ttcatgtttt aaattgttag taaatccatt accactaaat
      481 tcacctttta ttgagtgacc tgggccaccc attcctgtac catttgggca tcctccttgt
      541 atcataaatc ctggtattac tctatgaaat attattccat tgtaatatcc ttcatttact
      601 aatgatacaa agtttttaac tgtatttgga gctatatttg gatataactc aattttgatt
      661 tcttttccat tttccatttc tatagttact ataggatttt tattttccat aatttattac
      721 cttccttttc attagttaaa ttacataatt acatttatta tattatactc taaattaaag
      781 tctatcaagt atattttttc ctatttcgta tccttcttga taaatagttt tacaaaactc
      841 atcatcaaag ttaagcgtat ctttaggagc aaattgagta ttaggtctaa ctactattat
      901 tttatcttca tcatatactt ttttaatatc ctctggcaac tcataattat attttgtgga
      961 tattaaaata attttatcca cattatcccc aattaatggt tcaattaatg cactcctaat
     1021 taaacctcca tctaatttga aaccatcata aattccttct tgcacatctt tcacaaattg
     1081 ttctttaaat ccaagagttg cgtttggatt tgatggtaac aacccactgt atgaaatgct
     1141 gataatggct tcattacgag gcatttttga tatgtcaaca atcttttctg atactacttt
     1201 attatcaact tcaacataat taacataaaa gttcatttct tctatattac tatcatctag
     1261 ttcccttagg ttgtctatag caggagaatt atccacagta ttatttacta tttttacatt
     1321 tccattctgg atatctttta tatttgtcca cattttctcc aagttatcaa cattccctgt
     1381 gtacaaataa tatccattta ttgctcctat tgatgtccct gaaatcgaat aaaatttatt
     1441 tattcctcca tcatacaagg ctttaactac tcctgcttga taagcccctt ttgctcctcc
     1501 accttctaaa cataatccta atttcatagt ctcatctcca aattgtttat cttatattta
     1561 tatttcaaaa aacttactaa atgttttata tatactatag tggtcctcaa catttcctaa
     1621 ttctcttata gagtgcattg ctaaaattgg acttcccaca tccacagatg gtatatctat
     1681 atgagtagaa gatattggac ctatagtact tcctcctctt tcatctgacc tattaacaaa
     1741 ttcttgatac tcaacattaa cttctctaca tatattttta taaatagcta tagaatatcc
     1801 atcactagta tatgcctgac tagcatttat ctttataact ggaccttttc ccattattgg
     1861 tttattagtt gggtcatgct tttctggaat gtttggatga atagcatgag ctaaatctga
     1921 tgatatcata aaagatgaat aaatagactc aaagaatccc tctctatcct tacccattga
     1981 tatgcaaatt ctctccaata tatttaacaa catgtttgaa tctgcacctt gtttagtaga
     2041 gctaccaact tcttcattgt caaatacagc cattacattt acaccttgtt gacctttagt
     2101 attaacaaga gcattcaaac tagcatgtac cattgcaaga ttatcttgtc tcccaataga
     2161 tataaattca tcattagccc ctattaaact tcctttttca aattcatata aaaataaatc
     2221 aaaatctata atatcttcca tatttacatc taagttagat gctatttgct ttataagaaa
     2281 attatctttt tctaatgtat catttaacat cccaactaaa ggtaacatat ctttttgctt
     2341 attatatgaa tgaccatcat ttatacttct atttaaatgt attgctagat ttggtattat
     2401 acaaattggc tttttaaagt ctataagcct ttcatttggt tttaatatac tatcaccttt
     2461 taaaactact ctaccagcta tcccaagagg tctatccaac catgtactta atattgctcc
     2521 tccatagcat tctgtattta acttcaaata agatttttca gcttctattt ctgcatttgg
     2581 tttaatacta aatgtaggag aatctgaatg agagcctatt attctaaatc catctctttc
     2641 tatatcttct gaatttacta caaaagctac tatagctgat agattcttag taacataata
     2701 ctttccacca actcttagat tccatctttg attcaaaact aattcttcaa atccattttt
     2761 atctaagatt tccttagtac tctcgactgc atgaaaagac gacggactat catatatgaa
     2821 gtctattaag tttttagcaa agtttatact atccattcct catttacctc cattataaaa
     2881 tatttagtgt tgtatattct ttatttattt ttttaattcc ctgttggtca aaagctatag
     2941 taacatctgt accaattata gaaaccactg tcccaacccc aaatttagga tgatgtacct
     3001 tagaaccaag ttttatatca tctggattag tttctttatt agaatctttt attgtagcat
     3061 taactttatc tgcaacttta gatttgctta tagtactcat atacttttta gtatatttat
     3121 ctaacatatt ataacttgct ttagaatagc ttagttcttt ttttactttg tacaatcttt
     3181 ctatacattc ttctggcagt tcttccataa atcttgaagc tattgcaaca tttgttttac
     3241 catataatgt tctcttctct gtcaatgaca tatatagctc ttctttagct ctagttatac
     3301 caacataaca aagtcttctc tcttcttcta tttgtgaatc actcatagat tttatagctc
     3361 ttgatatagg aaataaccct tcttccatac ctgttaaaaa cacaacaggg aactctaaac
     3421 ctttggatgt atgaattgtc ataagcgaaa ccctatcatt gtcttcttca tcatttgatt
     3481 cagatgtaag tgctatactt gttaggaatg tctctaagtc tttattttca taagtatcat
     3541 cattactact ttcaaactcc aaagctatag aaataaattc tctcaaatta tcaattctgt
     3601 cttgagcttc ttcacctttg cctgttaaat ctttttcatt tttatttctt atctctacaa
     3661 gctcgtccat atatcctgta gtatccaaga ctttttctat aagtttgcta acaggataaa
     3721 cttcttttat agttctaaga gtccctataa tatcaacaaa tccacttata cttgcccttg
     3781 ccttagtaga tatatctgaa tttgtttcta tatcaattaa tactgagtat atactttcct
     3841 gttttaagtt agctctatct tcaatttttt ctatagttct aagccctata cctcttctag
     3901 gaacatttat aatccttttt atagatatat catcttgtgg attttgaata actcttaaat
     3961 acgccaacaa atcctttatt tcttttctct cgtagaattt tgtaccacca tatatattgt
     4021 atggaatttg acttctattt aaagcgtcct ctacaggtct tgcctgtgca tttgctctat
     4081 aaagtactgc aaaatcctta taaggtctat tttcttttct agctatttgt gcaattgaat
     4141 cagcaatgaa atcagcctct tctatttcgc taccagttaa ttgtatcttt ataagttctc
     4201 cttctttttt ttcactccaa agcttttttc tttttctttc tatattatta gatataactt
     4261 tattagctgc atctaatatc acttgtgtag acctataatt ttgttctaac tttacaacat
     4321 gtacattgtc ataatctttc tcaaattcaa gtatgtttct tatatctgcg cctctccatc
     4381 cataaatact ttggtcatca tctccaacaa cacaaatatt ttgatgttct cttgctaata
     4441 attttatcaa ttcatattga gctttacttg tatcttggta ctcatctacc attatgtatc
     4501 taaatctact tctgtaatat gctaatactt catcattagc cttgaaaagt tctacagttt
     4561 taagtattaa atcatcaaag tcaagtgcac tatttctttt taaacggtct tgatataacg
     4621 cataaatatc tgcaatctta accattctat tatctgccat gttgatatct ttaaactgct
     4681 ttggagtata aagtttgtct tttgctcctg aaattgcact tataactgct tttggttcaa
     4741 acactttatc acttaaattt aattctttta gacagtcttt aaccaatgta acttggtctg
     4801 ctgaatcata aattacaaaa cttctattgt aaccaatctt atttatatct tttcttagta
     4861 tacgaacaca gcaagaatgg aatgtactta tccacatatc cttagtttca ggacctatat
     4921 tttgttcaac tctctctctc atttcatttg cagctttgtt tgtaaatgtt atagctaata
     4981 tatttggtgc ttgtacacct ttatcttcta taagatatgc tattcttgtt gttaatacct
     5041 tagttttacc tgaaccagca ccagccagta taagtactgg accttcagtc ttttctactg
     5101 cttctctttg agcaggattt aatgtatcta aattcatatt tctcctcctg ttaacagcca
     5161 taaatatatt ttaatctaaa atatgaatta cttcaaatta taattatgta ttatattaat
     5221 agattatttc caatatatta caattaaata acatttttcc atataattta tttaattgtt
     5281 taataaaaaa aataacctta tgatttgggg aatcataagg cacctttaag gggtctaggt
     5341 taagggttat tatatataaa tctatttttt taaaataggt ttagggctta attatttatt
     5401 tgtattaagt tctctttcct tccaatactt atacaaatct tcttctttct taaataaggg
     5461 taaattcata tttctatgta cttttactaa ccatggggat tctaggccag cttgtataat
     5521 gttattttca tttacaaata cattttctgc ctttccttca tcaattaaaa tccccttatc
     5581 caatacatat atataatcgc atatttcata cattaaattc atatcatgac tggatattac
     5641 tatttttata ttatctttat tcaatccctt tattatatct acaatagacc tagtacttac
     5701 tgggtctaaa ccagctgtag gctcatcaag caatataatt tcattttcca ttgctataac
     5761 tgatgctatc gctacccttt ttttctgacc ataacttaaa aaatgtactg gtctatctat
     5821 aaaatctatt ccattaacag ctactagagc tttgtttatt ctctctttaa ttattttttc
     5881 atctatacca atatttctca ttgcaaaggc tatatcatca tatacttttg aataaaaaat
     5941 ttgtttttct gggtcttgga acacaatacc tacatttttt ctaaggttgt ataaacttct
     6001 cttatcatat tttaactttt cttctttaaa aagaatttct ccagatgtag gctttaatat
     6061 acccattaaa ttcataaata atgttgattt tcctgaacca tttgaaccta ttatacctat
     6121 aacatttccc tttgaaaaat ccatattaat atttaatagt gcattggtat tcttttcata
     6181 ttgataagtt aaattattta ttttaaacat cattatcccc tactatgtga aatttaccat
     6241 catacaattt tatgtctaat gatatacaca tatcatcata ccttgtcata agtcttttgt
     6301 acagcatact gccaagaatt cctaatgatt tatatgaagt ttttaaattt atgtacccaa
     6361 atcttagttc ttgtgactta tatatttctg aaaattcttc taaaaatatg aatataaacc
     6421 tatatattaa cattgatagc tcaacaaaag tatcaggcaa atgcattttt ttaaagaaga
     6481 atattaattg attaaaaggt gttgtaagta tacaaaagta tacacaagtc aaacaagaca
     6541 tagctctaaa taacacatga atagacatgt caatagatgc ttttgatatt cctacattga
     6601 aactaaaaat ttcaaaacta tataataagt ctgccttatt aaaagatata tttacaagag
     6661 ttattcctat actaagaaat aaaaagtaca ttggtattct gagtaatttt aaataattct
     6721 ttaaatctat tccagctaca caaacaacta gaatactcat taaaatcata attccactta
     6781 aaacaaatat attttttatt accatagaag caatcagaaa gattactcct atggctactt
     6841 ttttatttgg atttacttta cttaatctat ttgtatatgc atatttatca attataagca
     6901 taattaactt ctctttcctt tttgaacacc taatatgtac cctattacac cagcacctaa
     6961 cgctgcttgt gcagagaata ataaactttc tatttctcca cttggtggct catatgggct
     7021 actaaaccat ggttcataat ctggatttat cttagtaatt tcactctcag cttgaccatc
     7081 tgctcctcca tattcagctc ccgaattaac aagtaatggg aatatgataa gagctactac
     7141 taataaaagt agtaatatat tagttttcgt ctttgtttta gcactcatta gctttaactc
     7201 ctttatcatt attttctagt atgctttttt ctccattttc tgtaattaaa ttatatacaa
     7261 ttacagtaag aataccctct gctattgcta ttggtatttg agtcataaag aaaattccta
     7321 aaaacttaat tgctgaagcc atcactccac ctgatgggtc tgggaaagcc agtgctaact
     7381 gtatcgaagt tactgtatat gtagccaaat ctcctattgc tgctgcaaga aatactggca
     7441 ttgcattatt tccgtctttt ttctttaatg ctttaaatat taagaatgat ataattggtc
     7501 ctataatagc cattgaaaaa gcattagctc ctaaagtagt tattcctcca tgtgccaata
     7561 gtaatgcttg gaatattaga actatagttc caagaacaaa cattacactt ggtccaaaca
     7621 ttattgctcc aagacctact ccagtaggat gtgaacaact tcctgttact gaaggtatct
     7681 ttagtgctga caatacaaat acaaatgctc cacacagcgc taataatact ttctttttag
     7741 ggtcttgctt tactattttt cctattgatt ttagtcctac taaaaaaaat ggtataaata
     7801 taactcccca tgctatagac cattttactg gtaagtatcc ttccattatg tgcattgcat
     7861 tcgatgtaac tggagttaaa acgattaaca taagtgcagc tataacccct aatttgatat
     7921 tttgtttcat tttactatcc cctctctccc tttctaataa ttaaaatata aataatatat
     7981 gtctgattta ttttaaaaat aaaaataaaa aagcctaact cgatggctag gcttctaatt
     8041 ttcgataatc caaatacaaa cacataaaat gaacatatct gtataattat tataagaaac
     8101 tttctccatc cccgaagaaa ttactttaat attttaggca ggtctcctga cttagcttct
     8161 tcctactcct cttccttccc atgagatata aaaaatatca tcatagtggc taacaaaagt
     8221 ttcgtctgca tcacagtagc gggggctgta aaggaattta acctttttcc ctattaatct
     8281 tgcttaaaga acctataata ttatatattt aatttttaaa ttattcttaa atcattttct
     8341 atataaatca ttgtatacct tttctgtgaa aatttctaca ttttttataa attttattca
     8401 ttt
//
LOCUS       DAMJWP010000157         8287 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.162, whole genome shotgun sequence.
ACCESSION   DAMJWP010000157 DAMJWP010000000
VERSION     DAMJWP010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8287
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.162"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(31..1422)
                     /locus_tag="PC700_002730"
     CDS             complement(31..1422)
                     /locus_tag="PC700_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HDF2582027.1"
                     /translation="MNFRDLFEKAVDFIDEKRKSIVAISLSTLGVIALIIVFFLSSNE
                     FSVGNEANELLKIIEKRQYSIAVDYYTSIEKKFSDSKMERFNKSVSKKINKLLLNSGD
                     KYLDGDISQESFIGLINTVKELNKISIDVDDLLAQADRVREMYKEENTTYDIAINYIS
                     TVSILNGMGSNLDVYKQNIETIKESRDVYDSAVKDQKAYKYYEAIEKYNKVLKEDEKY
                     YSMAQNGKEQCIEEMYDYYISRAEEANTSGDYERALQYIEYLKEDYSDDEKVQSLEKK
                     YKKNLSLYTLTSDDIINVIAKKSGKDKANLSINSLPQMIKNNKYYYAEVYEYDKLINE
                     VLVSAKTKDLYSYKDGKKDYKVDYGDGYFRILEDGSYQFGITKDKAKFVLTNTLDEKE
                     NKYKKIEILDIEKADRYVKSKKSLEELFGKYKNIYYYAVVNKGLFRGKEVYAINIYNE
                     KIYAISENGLNEY"
     gene            complement(1588..1836)
                     /locus_tag="PC700_002731"
     CDS             complement(1588..1836)
                     /locus_tag="PC700_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582028.1"
                     /translation="MMICFAMTFALVLINSVMGVHIGLDYYFLMGSVLTVCLTIGIVL
                     CNARKIFDSKAIHKTRTKTVSTNKKQKSHSRDIKRKIS"
     gene            2102..4231
                     /locus_tag="PC700_002732"
     CDS             2102..4231
                     /locus_tag="PC700_002732"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HDF2582029.1"
                     /translation="MPNLPSLGSKAPDFKANTTNGPIRLSDYKGNWIVLFSHPGDFTP
                     VCTTEFLCFAKYYDEFKKRNTELIGLSVDSNSSHLAWMYNISLLTGVEIPFPIIEDRD
                     MRIAKLYGMISKPMSDTSTVRSVFIIDNNQILRTILYYPLTTGRNIPEILRIVDALQT
                     SDRDNIVTPANWFPGMPVILPYPKNYKELKNRVNSCNKKYSCMDWYLCFVPDNYNDEE
                     VSKKIDNTCSWKKEHTKNIENECNCEHEHHDYLNKALDCKQEHKTDIKDDCNHEKKHT
                     KNTNKVHNSKQDKFKDKSCDEMNFNYDKDESCDKINSSYNKEDSSYEDFYKHNYKNYD
                     YTSEKNTKKIAMKTLKDSKKLVRPQITDPYNPIVENANCPDINPIVAEYVLGNPTNVD
                     AQLLDAVIFAFAEIDQSGNLFIPYPRFLNQLLALKGEKPSLKVIVAIGGWGAEGFSDA
                     ALTPTSRYNFARQVNQMINEYALDGIDIDWEYPGSSASGITSRPQDRENFTLLLTAIR
                     DVIGDDKWLSVAGTGDRGYINSSAEIDKIAPIIDYFNLMSYDFTAGETGPNGRKHQAN
                     LFDSDLSLPGYSVDAMVRNLENAGMPSEKILLGIPFYGRLGATITRTYDELRRDYINK
                     NGYEYRFDNTAQVPYLVKDGDFAMSYDDALSIFLKTQYVLRNCLGGVFSWTSTYDQAN
                     ILARTMSIGINDPEVLKEELEGIYGQF"
     gene            complement(4488..5087)
                     /locus_tag="PC700_002733"
     CDS             complement(4488..5087)
                     /locus_tag="PC700_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HDF2582030.1"
                     /translation="MAKQKFYAVKKGKNIGVYNTWDECKKQVNGFSGAEYKSFSTFQE
                     AKEYIDGSEKLSFQEDKEFIEAYVDGSYEHSVKMYGSGVVILKNNEVIKTYSEKGKEK
                     TLVSMRNVAGEIEASKIAMQYCIDNNVQNLILYFDYEGIEKWCTGVWKTNKEGTIAYK
                     NFYDSIKNKLNVKFTKVKAHSGNKYNEEADKLAKKAIGV"
     gene            complement(5105..8224)
                     /locus_tag="PC700_002734"
     CDS             complement(5105..8224)
                     /locus_tag="PC700_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDF2582031.1"
                     /translation="MDNIISVLNDVVFLDIEVSGLDCLNSEILEVGAVKVKDWKIYTY
                     ESLIKNKFEVPIEVFSVCKNLDKNDLEIANEIELVEDRLVNFVEDSFIICHDLSLKKK
                     FFEYHMPKLKNKFIDLIELAVILEPYHKDYSLEYLKNTLTNCNSKVENRALSDAIDII
                     NIVNCLLVKFNNYEKTTLEPLSFKINSYLKKFNLPTWEWSKFLEEANYDLSNNINIKK
                     EYNIFDSKEEKKKERETLKILNEEEKNYEELLKYKTIWENKEGFTYEYRPGQYELTKT
                     IRELFRNSEDEEKIACIEAPTGIGKSVGYLLPAILEARINKKRLLISTDTKELQIQLI
                     NKDIPNVLDSLGLNGKVSYGYIKGKNNYICIDRLEAYIDDYESQNPTKGEILSLIFLK
                     RLVEGGKYGDIEEINYLVFDNFKEISTHLRNVSCDPNMCRPKKCKKDCLYKNRIEELK
                     EEHITVVNHSLLAKWPYKDEKPLENIIVDEAHNLTEKGYDFFSSIINSKSLRYLLQEI
                     YPYEFIQNSSFIYKKYSRNMRKIKAFDKFYNVLKIGREDKQKIARSINLIIEEIDSIL
                     KFGNCNEYNNVSNYNLRWELNLQIDEIVGKLKKDGIDTEISYRAYSEKIKLSCEKIIK
                     NLVSIIIIIYRNIDDDSIDKEADIYKFGKAKTRDLEDIKIIFEIFLEYDEKDDYARIV
                     EIDKNYNDFEFRVVPLKIADLFEENILSQLEKGIFLSATLSLSESMSYFKNTLGIDRV
                     KNVEKIIEPIFDYKNRVSVVGFSDICEYRNSEFPNEMSKIISNISKITEGHTLALFNS
                     KDRQEKTYEILKKYLHSFNLEIYADKKGIRHLNDLNRKCVVLGSKGCFEGVDIPGDGL
                     VCVTLDKLPNLNPKDPLYFTIMKKYGIDYYTINYPQMTIKVKQAMGRILRSKYDYGCF
                     VIFDVGTNISVLKRLEKDLHDCKISKVNSNEFYTYIRRHLNKSRSLILKSVIFDTIKA
                     LNVDAKMDNNDVDKDIIKKDINENIRQRAVKGEVYHIDIIKKDMKVKYFDRNYLINLD
                     IFMREEDKN"
BASE COUNT         2791 a         1336 c          846 g         3314 t
ORIGIN      
        1 tttaattata tattaattta atattattaa ttaatattca tttagtccat tttctgatat
       61 ggcatatatt ttttcattgt aaatattaat agcataaact tctttgcctc taaataagcc
      121 tttatttact acagcataat aataaatatt tttatatttt ccaaacaatt cttctaaact
      181 ctttttactt tttacatatc tatctgcttt ctctatatct aatatctcaa tctttttata
      241 cttattttct ttttcatcaa gagtattagt taacacaaac ttagctttat cttttgtaat
      301 accaaattga taacttccat cttctaatat tctaaagtat ccatcaccat aatctacttt
      361 gtaatctttt ttaccatctt tatacgaata taaatcttta gtttttgcac ttaccaaaac
      421 ttcatttatt aatttatcat actcatatac ttcagcataa tagtatttat tattttttat
      481 catttgtgga agtgaattta tagatagatt tgccttgtct ttgccacttt ttttagctat
      541 tacatttata atgtcatcag atgttaaggt atacaatgat aaattctttt tatatttttt
      601 ttctaaactc tgtacctttt catcatctga ataatcttct tttaaatact caatatattg
      661 taatgctctt tcataatctc cagaagtatt tgcctcttca gctctactta tatagtaatc
      721 atacatttct tcaatacatt gttctttccc attttgagcc atactgtaat acttttcatc
      781 ttcttttaat actttattat acttttcaat agcttcataa tacttatatg ctttttggtc
      841 ttttactgct gagtcataaa catctctaga ctctttgatt gtttctatat tctgtttgta
      901 tacatctagg ttactaccca taccatttaa tattgaaaca gtacttatgt agttaatggc
      961 aatatcataa gtagtatttt cttctttata catttctcta actctatcag cttgagctaa
     1021 taagtcatcc acatctatac ttattttgtt taattctttc acagtattta ttaatccaat
     1081 aaaactttct tgacttatgt ctccatctaa atatttatca ccactattta atagcaattt
     1141 atttattttt ttagaaacag acttattaaa cctttccatt tttgaatcag aaaacttttt
     1201 ttctatactt gtgtagtaat caacagctat agaatactgt cttttctcta taatctttaa
     1261 caactcatta gcttcatttc ctacactaaa ctcgttacta cttaagaaaa atactattat
     1321 caaagcaatt acacctaatg ttgataatga aatcgcaact atagatttcc ttttttcatc
     1381 aataaaatct acagccttct caaataaatc tctaaaattc attatagttc cctctccatt
     1441 taataatctt tatattttat ggtaaactaa atttcttatt ggttattata tcataattct
     1501 tgaaaaaaaa taaaaacaca agttaaatcg tgtttttatt ttacctatca atcattattt
     1561 tatacataaa tatttccttt acataaatta tgatatcttt ctttttatat ccctagaatg
     1621 actcttttgt tttttattag tactgacagt tttcgttctt gtcttgtgta tagctttgct
     1681 atcaaatatt tttctagcat tgcataaaac aattccaatt gttaaacaaa ctgtaagcac
     1741 acttcccatc aaaaagtaat aatccaatcc tatatgtact cccataactg aatttattaa
     1801 cactaatgca aaagtcattg caaaacatat catcatggag ataactaaaa ttcttagaat
     1861 ttttttcaat ccaatcacct cgtataatca ctaaaattga cactacccct tatccattac
     1921 tataccaaat tagtaaaaaa atagcaatta ctattgtcat ttatattagg aaaatattaa
     1981 atctttttaa taaatatctt tttttgacat aaggattact taacttttaa taaaatagta
     2041 acactaatat gtattaatta atagaataaa ataaatgata atttattgag gggtgattta
     2101 catgccaaat ttgccaagtt tagggtcaaa ggctcctgat tttaaagcca atacaacaaa
     2161 tggtcctatt agactctctg actataaggg taattggatt gttttatttt cacatcctgg
     2221 tgattttaca ccagtttgta ctacagaatt tttatgtttt gctaaatatt atgacgaatt
     2281 taaaaaaaga aatacagaac taattggtct aagtgttgat agtaacagtt cacatttagc
     2341 ttggatgtat aatatttctt tacttacagg tgtagaaatt ccatttccta ttatagaaga
     2401 tagagatatg agaattgcca agttatacgg catgatatca aaaccaatga gtgatacatc
     2461 aactgttcgc tctgtattta ttatagataa taatcaaatt ctaagaacga ttctttatta
     2521 tccactaact acaggaagaa atattccaga aatacttaga atagtagatg cacttcagac
     2581 tagtgataga gataatatag ttactcctgc aaactggttt cctggaatgc cagtgatttt
     2641 accttatcct aaaaactata aggaattaaa aaatagagtt aacagttgta ataagaaata
     2701 ttcatgtatg gactggtact tatgttttgt accagataat tataatgatg aagaagtgag
     2761 caagaaaatt gataatactt gtagctggaa aaaagaacat actaaaaaca ttgaaaatga
     2821 atgtaactgt gaacatgaac atcatgacta cctgaacaaa gctcttgact gtaaacaaga
     2881 acacaagact gatattaaag atgattgcaa ccatgagaaa aaacatacta aaaatactaa
     2941 caaagttcac aactccaaac aagataagtt taaagataag tcttgtgatg aaatgaattt
     3001 taactatgat aaagatgaat cttgcgacaa aataaattct agctataaca aagaagatag
     3061 tagttatgaa gatttctata aacataatta taaaaactac gattatacta gcgaaaaaaa
     3121 tactaaaaaa atagctatga aaactttaaa agattcaaaa aaattagtta gaccacaaat
     3181 aacagaccca tacaatccaa tagttgaaaa tgcaaactgt ccagatataa atccaattgt
     3241 agcagaatat gttcttggaa atccaactaa tgtagatgct caactattag atgcagttat
     3301 atttgctttt gctgagatag accagtctgg aaatttgttt attccttatc ctagattttt
     3361 aaatcaatta cttgctctta aaggtgaaaa acctagctta aaagtaattg tagctattgg
     3421 aggttgggga gctgaaggat tctctgatgc agcattaaca cctacatcta gatataattt
     3481 tgcaagacag gtcaatcaga tgataaatga atatgcttta gatggaatag atatagactg
     3541 ggaatatcct ggaagtagtg catctggaat aacatcaaga cctcaagata gagaaaactt
     3601 tacactctta ctaactgcca taagagatgt tataggagat gataaatggc ttagtgtagc
     3661 tggaacagga gatagaggat atataaattc aagtgctgaa atagataaaa tagctcctat
     3721 aatagattat tttaatctta tgagttatga ttttacagca ggtgaaacag gcccaaatgg
     3781 tagaaaacat caagcaaatc tttttgattc agacttatct ttgccaggat atagtgttga
     3841 tgcaatggtg agaaatcttg agaatgctgg aatgccttct gaaaaaatcc ttctcggtat
     3901 accattttat ggaagattag gtgccactat aacaagaact tatgatgagc ttagaaggga
     3961 ttatataaat aaaaatggat atgaatatag atttgataat actgctcaag ttccgtattt
     4021 agttaaggat ggagattttg caatgtcata tgatgatgct ttatcaatat tcttaaaaac
     4081 tcaatatgtt cttagaaatt gtctaggtgg tgtattctca tggacatcaa cttatgacca
     4141 agcaaatata ctggctagaa ccatgtctat tggtataaat gaccctgaag tattaaaaga
     4201 agaacttgaa ggtatttatg ggcaattcta atagaatgta ggaagatttt atagttatat
     4261 aaaaagcaga ttttattata ttaaataagt tgtatctaat taatactatt attaattgtt
     4321 tataaattag atacttattt tttcataata caatctgctt ttttaatcaa aatttattta
     4381 attataagtt gtttaattac aagttgttta attacaagtt gtttaattat aaagtaggaa
     4441 tttagatata ctcttatacc atgtaattat gtataaatat ttttttatta tacaccaata
     4501 gcttttttag ctaatttatc agcttcctca ttatacttat ttccagaatg agctttaacc
     4561 tttgtaaatt ttacattaag cttattctta atactgtcat agaaattttt atatgctata
     4621 gttccttctt tatttgtttt ccaaactcca gtacaccact tttcaatccc ttcatagtca
     4681 aagtataaaa tcaagttttg aacattattg tcaatacagt actgcattgc aattttagaa
     4741 gcctctatct ctccagctac atttctcata cttactaaag ttttctcttt tcctttttca
     4801 ctataagttt ttataacttc attatttttt aaaatcacaa cacccgaacc atacattttt
     4861 actgaatgtt cataacttcc atctacatat gcttctataa actccttatc ttcttgaaaa
     4921 ctcaattttt ctgacccatc aatatattct ttagcttctt gaaaagtaga aaaactctta
     4981 tattcagctc ctgagaatcc atttacttgt tttttgcact catcccatgt attatataca
     5041 cctatatttt tacctttttt cacagcataa aacttttgtt ttgccaaata tctcactccc
     5101 actcttaatt tttatcttct tctctcataa atatatctaa atttataaga tagtttctat
     5161 caaaatattt tactttcata tcttttttta taatatcaat atggtacact tctcctttta
     5221 cagctctttg tcttatattt tcatttatat ctttttttat aatatcttta tcaacatcat
     5281 tgttatccat tttagcatca acattcaacg cttttatagt atcaaatata acagatttta
     5341 gtattaaact tctagattta tttaaatgtc gccttatata agtataaaac tcattgctat
     5401 taacctttga aattttacaa tcgtgtaaat ctttctctaa tctctttaaa acacttatat
     5461 tagttcctac atcaaaaatc acaaaacatc catagtcata tttgcttctt aaaattctcc
     5521 ccatagcttg ctttaccttt atggtcattt gaggataatt tatagtatag tagtctatac
     5581 catacttctt cattatagta aaatacaaag ggtcttttgg atttagattc ggaagcttgt
     5641 ctaaagttac acaaaccaaa ccatcccctg gaatgtctac gccttcaaaa catccttttg
     5701 agccaagtac tacacatttt ctatttaaat cgttaaggtg tctaatcccc tttttatcag
     5761 cataaatttc taagttaaaa ctatgtaagt attttttcaa tatttcataa gttttttctt
     5821 gtctatcttt actattaaaa agagctaatg tatgaccttc tgtaatcttt gatatattag
     5881 atataatttt actcatctca tttggaaatt cagaatttct gtactcacaa atatcagaaa
     5941 atccaactac agagactcta tttttataat caaaaattgg ttctataatc ttttccacat
     6001 tcttaactct gtctattcct aatgtatttt taaaataaga catactttca gataaactca
     6061 aagttgctga taaaaatatt cctttttcta attgagataa aatattttct tcaaataaat
     6121 ctgctatttt taatggtact actcgaaatt caaagtcatt ataattctta tcaatttcaa
     6181 ctatccttgc ataatcatcc ttttcatcat actctaaaaa gatttcaaat ataatcttta
     6241 tatcctctaa atctcttgtc tttgcctttc caaatttata tatatctgct tctttatcta
     6301 tactatcatc atcaatattc ctataaatta ttattattat tgaaactaaa ttttttatta
     6361 ttttctcaca actcaacttt atcttttcac tataggctct ataacttatt tcagtatcta
     6421 taccatcctt ttttagtttt cccacaattt catctatttg caaattcagt tcccatctta
     6481 aattataatt acttacatta ttatattcat tacaattacc aaactttaat attgaatcta
     6541 tctcttcaat aatgagattt atacttctag caattttttg tttatcttct cttccaattt
     6601 ttaatacatt ataaaattta tcaaaagcct ttatttttct catatttcta ctatattttt
     6661 tatatataaa gctactattt tgaataaact cgtaaggata aatttcttgt aataaatacc
     6721 ttaaagactt tgaatttata atgcttgaaa agaaatcata tcctttttca gtcaaattat
     6781 gtgcttcatc tactattata ttttctaaag gtttttcatc cttatacggc catttagcta
     6841 atagtgaatg atttactact gttatatgtt cttcttttaa ttcttctatt ctgtttttat
     6901 ataaacaatc ttttttgcat tttttaggtc tgcacatatt tgggtcacaa cttacatttc
     6961 ttaagtgggt acttatttcc ttgaagttgt caaacactaa ataatttatt tcttctatat
     7021 ctccatactt gccaccttca actaatctct tcaaaaatat taaagataaa atttctcctt
     7081 tagtagggtt ttgagactca taatcatcta tatatgcttc cagtctatct atacaaatat
     7141 agttattttt tccctttata tatccgtaac ttacttttcc atttagaccc aaagaatcta
     7201 acacatttgg aatatcttta tttattaatt gtatctgcaa ttcctttgtg tcagtagaaa
     7261 ttaataatct ttttttatta attctagcct ctaatattgc aggcaataaa tatcctacac
     7321 ttttacctat accagttggt gcctctatac atgcaatttt ttcttcatct tcgctatttc
     7381 taaacaactc tcttattgtt ttagtaagct catattgacc aggtctatat tcatatgtaa
     7441 atccttcttt attctcccaa atagttttgt attttaataa ttcttcataa tttttttctt
     7501 cttcatttaa aatttttaag gtttctcttt ccttcttctt ttcttctttt gagtcaaata
     7561 tattatattc tttttttata tttatattat ttgataaatc ataatttgcc tcttctaaaa
     7621 atttgctcca ttcccatgtt ggaagattaa attttttaag atatgaattt attttaaaac
     7681 tcaatggctc caaagtagtt ttttcatagt tattaaattt tactaaaagg caatttacaa
     7741 tatttattat atcaatagca tctgataaag ctctattttc cactttagaa ttacaatttg
     7801 ttaaagtatt ttttaagtat tctaagctat aatctttatg atatggttct aaaataactg
     7861 ctaattcaat taaatcaata aatttgtttt ttaactttgg catatgatac tcaaaaaact
     7921 tttttttcag acttaaatca tgacatataa tgaaactatc ttctacaaaa tttacaagtc
     7981 tatcttccac cagttctatt tcattagcta tttctaaatc atttttatct aaatttttac
     8041 atactgagaa tacttctata ggtacctcaa atttgttttt tattaatgat tcataggtat
     8101 atattttcca gtctttaacc ttgacagctc caacttctaa aatttctgaa tttaaacaat
     8161 ctaatccact aacttctata tctaaaaaaa caacatcatt caatacactt attatattat
     8221 ccatttttat atcccttcaa gttaaaccag cctaacttat ttacaatcat ttctctttat
     8281 attttat
//
LOCUS       DAMJWP010000158         8259 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.163, whole genome shotgun sequence.
ACCESSION   DAMJWP010000158 DAMJWP010000000
VERSION     DAMJWP010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8259
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.163"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            65..1051
                     /locus_tag="PC700_002735"
     CDS             65..1051
                     /locus_tag="PC700_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF2582032.1"
                     /translation="MKKITINDIANLAGVSKSTVSRYLNNKDISDSTKEKIKTIIDEY
                     GYEPNAFAQSLRAKKTYFIGIITPCLDSFVKSKIMMAIDEELKELKYTSLIINTNRKI
                     RSEIDSISKLASLKVDGIILIGTEITKEHKNEIEKLDIPMVVVGQKVDGINSIVNDDY
                     GAGYKMGQYIANKGYKNIVYLGVDESDISVGLNRKNGVLNGLKDKGYDAKVFYTDFDQ
                     ETSIQRSGEMLESENPDIIICATDNIAIATMKEINKRGKNIPQDISVAGFGGYDVLSI
                     ISPKLTTIKFENKNAGKVAANTIVNLIQERKEPLLKEIKFELIEGESTINKN"
     gene            1274..1357
                     /locus_tag="PC700_002736"
                     /pseudo
     CDS             1274..1357
                     /locus_tag="PC700_002736"
                     /inference="COORDINATES: protein motif:HMM:NF012587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
     gene            1388..2869
                     /locus_tag="PC700_002737"
     CDS             1388..2869
                     /locus_tag="PC700_002737"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDF2582033.1"
                     /translation="MYKEPKYRTILESIKEELNKLRNISLNDKYKPLFHIHPQHGLLN
                     DPNGLAYYNGKYHVFYQWYPYDATHGMKHWAYVSSDDFVNWNREDVALIPIESYESHG
                     AYSGNAIEVDGKLHMYYTGNIKYSAEDRYAYQNLAIMNKDGKITKYENNPIVSEIPKG
                     YTGHVRDPKVFKRKDKYFMLLGAQTSDKKGVIIVYESKNSIDWNFKGELNVKNIDEDF
                     GYMWECPDYINIDEKDILIFSPQGVEPKGFDYQNIYNVVYAIGNMDLDNLTFEIDTMK
                     ELEKGFDFYAPQTFIKDSQIILFAWAGMGEVLYPTDKNKWAHCLTVPRKLNIKNNKLL
                     QMPVDELIKLRYDETSGQNTIKNNINIIENDENVYELNINIKNIDSNKFGLELFSSQD
                     EGVKLEFNKLGNIVTLDRSNFKKVFGVEYGTNRKEYINIDENTNIKVLADRSVLEIFI
                     NDGEVVFTSRIFAKENSNQIRVYSDKIVEYEYTKFKLKQGIEL"
     gene            2895..3866
                     /locus_tag="PC700_002738"
     CDS             2895..3866
                     /locus_tag="PC700_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HDF2582034.1"
                     /translation="MKKVISIGEALIDFIPNQNGCKLKNVSEFRRVAGGAPANVSVVV
                     AKLGGKSSFISKLGKDAFGDYIIDVLNEVNVNTDYVLRTSKANTGLAFVSLKEDGNRD
                     FSFYRNPSADMLLEADEVKKEWFNNCHILHFCSVDLIDSPMKLAHKKAIEYALESNSI
                     ISFDPNIRLPLWDSEQSCKKAISEFLPFAHIVKISDEELEFMTGENDIEKSLHKLFVG
                     NVELVLYTKGKDGVDAYTKKVKGMCKGVKVNAIDTTGAGDSYIGSFLYTLLYKQITLN
                     DIKEMKQETLNEYLEFSNYYAARSTTIKGAISSYATKEEITEFIKNL"
     gene            complement(4122..4724)
                     /locus_tag="PC700_002739"
     CDS             complement(4122..4724)
                     /locus_tag="PC700_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2582035.1"
                     /translation="MEICILMGSPKKKGNTAAILKPFIEELENYNSNIEVIWLYDCKI
                     EPCIACKKCQDNLFDFGCYCKDDVQKIFKKILMCDLIVFATPIYSWYCTSPMKALLDR
                     LVYGMNKYYGNEKGPALWAGKPVALITTCGYRPEKGADIWENGMKRYCKHSQLKYIGM
                     LVERDLGKPNFMNKEKEKHSRQFAKQIYYILDNSIEVDNC"
     gene            5005..6216
                     /locus_tag="PC700_002740"
     CDS             5005..6216
                     /locus_tag="PC700_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HDF2582036.1"
                     /translation="MYSNEDYKFIEENAPCYIYDETNIIEKCKELKNNILGFDFLYSI
                     KTNPYNKIVKSIAEQGFGADASSTNEVMISSECGVSKYDIYYSSPGKTEIDIETVLDK
                     CIIIADSITEIELINKISKKHNQKVKIGIRINPDFTMDDDSAKSSKFGIDIEDMSSVF
                     KMLSMCDYVSVVGIHIHIKSQVLDFEKLGHYYEKCFFIALNLNKSKYINIEFINFGSG
                     LGVAYDNLRENPLNLKELSKLTQKIVFTNSNTLKSRLLIESGRFIVCKSGNYYVKIVD
                     IKVSRGKKYLIVKNGLNGFMRPIISNLIENITCGVELEGQEPLYTSNHAFSLSVLNNL
                     KEEEYVNVVGNLCTSLDVIANNVKLKKANIGDLISISNAGSYSYSLSPLLFSSYEFPK
                     QYYKRKDGYIE"
     gene            6284..6481
                     /locus_tag="PC700_002741"
     CDS             6284..6481
                     /locus_tag="PC700_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HDF2582037.1"
                     /translation="MELWKRNLFVCWIGMFFSSIGMSQIAPILPLYIKQLGVTDVSLI
                     QQYSGIIFGCTFVVAAFFSPI"
     gene            6515..7486
                     /locus_tag="PC700_002742"
     CDS             6515..7486
                     /locus_tag="PC700_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2582038.1"
                     /translation="MLLRASIGMGIVIFTMAFVQNVYQLLGLRILQGVFTGYATACTT
                     LIATQTDKNHSGWALGTLATASTTGSLIGPTVGGYIESILGLKSTFIITGGLLFVSFI
                     ISILFVVDNFKPKEIKESISIPKEQLNILPNKFLIASIFVTTFITQLALYSIEPIVTI
                     YISQLTNFASNVALIAGLTFSASGLANLLAAQKLGKMSDKIGPQKVLLISLLWAGIIF
                     IPQAFVKTAWQLMLLRFLLGLSVAGLNPSVNSLLKKIAPEEYVGKIFGYNASAQYIGC
                     SSGAFLGGQISAHLGIRTVFFSTSLLLFMNALWMYKFTGLFIKDKTK"
     gene            7673..8050
                     /locus_tag="PC700_002743"
     CDS             7673..8050
                     /locus_tag="PC700_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2582039.1"
                     /translation="MTISKIPQAELKVMKFIWEKNDTVTSKETIEAMERKYGWKQTTT
                     LTLLSRLVKKHFLSAEKIDRYTHYTIVVGHKEYLSVETKDFLSNIHDNSLQSLISALH
                     DDEVVDKDMLDLFESHFKNLEEE"
BASE COUNT         3345 a          859 c         1335 g         2720 t
ORIGIN      
        1 atattttatt ttaaatatat aatataatgt aataaaatat atcaaatgta gaaggtgata
       61 acctatgaaa aaaataacaa taaatgatat tgctaacttg gcaggagtat ctaaaagtac
      121 tgtatcaaga tatctaaata ataaagatat cagtgattct actaaagaga aaataaagac
      181 aataatagat gaatatggat atgagccgaa tgcatttgct caaagtttga gagctaaaaa
      241 aacatacttt attggtatta taacaccgtg tttagattct tttgtaaaat ctaaaattat
      301 gatggcaata gatgaagaac tcaaagaatt aaaatataca tcactaatta ttaatactaa
      361 tagaaaaata aggtcagaaa tagatagtat atcaaagtta gctagtctaa aagttgatgg
      421 aataatacta attggtaccg aaataactaa agaacataag aatgaaattg aaaagttaga
      481 tataccaatg gttgtagttg gacaaaaagt tgatggtatc aatagcatag tgaatgatga
      541 ttatggagca ggatataaaa tgggtcaata tattgctaat aaaggttaca aaaacattgt
      601 atatttaggt gttgatgaaa gtgatatatc agttggttta aatagaaaaa atggagtact
      661 taatgggtta aaagataaag ggtatgatgc aaaagtattt tatactgact ttgaccaaga
      721 aacgtctata caaagaagtg gagaaatgct agaaagtgaa aatccagata taataatatg
      781 tgcaactgat aatatagcaa tagcaacaat gaaagaaatc aataagagag gtaaaaatat
      841 accacaagat atatctgtag ctggatttgg gggatatgat gtattgtcta ttataagccc
      901 taaacttaca acaataaagt ttgaaaataa aaatgcaggt aaagtagcag caaatacgat
      961 agtgaactta atacaagaaa gaaaagaacc actattaaag gagattaaat ttgaattaat
     1021 agaaggtgaa agtacaataa ataaaaatta agatacacat tacatatatt agtaatgtgt
     1081 atttttttac taaaaaaact ataattctaa aaattaggaa aacttttttg aaagaaatat
     1141 tgcatctgaa ataaatttat gctaatcttt aattaaaccg gtttcgaaac cggtttaatt
     1201 aaaccgaaag aatagcacaa tgagtgcaaa aataactgat tgtaacaaag tatcaaagta
     1261 attattagag tacataggaa ataaacaaaa tataaaaggt gtagcacatt gtgtaacaag
     1321 attaagaatt gttttagata gtaataataa agcaaatata aaattacaaa agaaaaggaa
     1381 taaaaatatg tacaaagagc ctaaatatag aaccatatta gaatcgataa aagaagaatt
     1441 aaataaatta agaaatatat ctttaaatga taaatataaa ccactatttc atatacatcc
     1501 acaacatgga ctacttaatg atccaaatgg attagcatat tataatggta agtaccatgt
     1561 attttatcaa tggtatccat atgatgcaac tcatggaatg aagcattggg cttatgtaag
     1621 ttctgatgat tttgtaaact ggaatagaga agatgtagca ttaataccaa tcgaaagtta
     1681 tgaatctcat ggagcatatt caggtaatgc gatagaggta gatggaaaat tgcatatgta
     1741 ttatacaggg aatataaaat atagtgcaga agatagatat gcatatcaaa acttagctat
     1801 aatgaacaag gatggtaaaa taactaaata tgaaaataat ccaatagtaa gtgaaatacc
     1861 aaaaggatat acaggacatg taagagaccc aaaagtattt aaaagaaagg ataaatactt
     1921 tatgttactt ggagcacaaa caagtgataa aaaaggagtt ataatagtat atgaatctaa
     1981 aaactctata gattggaatt ttaaaggaga attaaatgta aaaaatatag atgaagattt
     2041 tggttacatg tgggaatgtc cagattatat taatatagat gaaaaagata tattaatatt
     2101 ttcgccacaa ggagtagaac ctaaaggatt tgattatcaa aatatatata atgtagtata
     2161 tgctattggt aatatggatt tagataattt aacatttgaa atagatacaa tgaaagaact
     2221 ggaaaaagga tttgatttct atgctccaca aacatttata aaggactcac aaataatact
     2281 atttgcatgg gctggaatgg gagaagtttt ataccctaca gataaaaaca aatgggcaca
     2341 ttgtctaact gttccaagaa aattaaatat aaaaaataac aagctattgc aaatgcctgt
     2401 agatgaatta attaaattaa gatatgatga aacaagtggg caaaatacta taaagaataa
     2461 tatcaatatt atagaaaatg atgaaaatgt atatgagctt aatattaata taaaaaatat
     2521 tgacagcaat aaatttgggc tggaattatt ttcatcacaa gatgaaggtg taaaattaga
     2581 atttaacaaa ttaggaaaca ttgtaacttt agacagaagc aactttaaaa aagttttcgg
     2641 tgttgaatat ggaacaaata gaaaagaata tataaatatt gatgaaaata ctaatatcaa
     2701 agtactagcg gatagaagtg ttttagaaat ttttataaat gatggagaag tagtatttac
     2761 tagtagaata tttgctaaag aaaattctaa tcaaataagg gtttatagtg ataaaatagt
     2821 agagtatgag tatactaaat ttaaattaaa gcaaggtatt gaattataaa aacacattat
     2881 aaggggtaga aaagatgaaa aaagtaatta gtataggaga agctttaata gactttatac
     2941 ctaatcaaaa tggttgtaaa ttaaaaaatg tatcagaatt tagaagagta gcaggaggag
     3001 ctccagcaaa tgttagtgtt gttgtagcaa agcttggagg aaaatcaagt tttatatcta
     3061 agttaggaaa agatgcattt ggtgattata taatagatgt attaaatgaa gtaaatgtaa
     3121 atactgatta tgtattgaga acaagtaaag caaatacagg acttgctttt gtatcattaa
     3181 aagaagatgg taatcgtgat ttctcatttt atagaaatcc aagtgcagat atgcttttag
     3241 aagctgatga ggttaaaaaa gagtggttta ataattgtca tatcttacat ttttgttcag
     3301 tagatttaat agatagtcca atgaaattag cacataaaaa agcaatagaa tacgcacttg
     3361 aaagtaatag tataataagt tttgacccta atataagatt acctttatgg gatagtgagc
     3421 aatcttgtaa aaaggcaata tcagaatttt taccttttgc tcatattgtt aagatatctg
     3481 atgaagaatt agaatttatg acaggagaaa atgatataga aaaatcatta cataaactct
     3541 ttgtggggaa tgttgaacta gtattatata caaaaggaaa agatggagta gatgcataca
     3601 ctaaaaaagt aaaaggtatg tgtaaagggg ttaaagtaaa tgcaatagat acaaccggag
     3661 caggagattc ttatataggt tcatttttat acaccttatt gtataaacaa ataacattga
     3721 atgatataaa agaaatgaaa caagaaacgt taaatgagta tttagaattt tctaattatt
     3781 atgcagctcg tagtacaact attaaaggtg cgataagttc atatgcaaca aaggaagaga
     3841 taactgaatt tataaaaaat ttatagtagg aatgagtaat aggttttgaa ggaagtaaca
     3901 tgtaaagagt ttttaaggat gtttactatg aaaataatta acatataaaa agcaataaag
     3961 aggctgtctc aaaatagata taagcctctt tatcattgtt gaaatgataa aaaatatttt
     4021 aattattgtt actttttaga agaatatata tcttatttta aataagcaaa ttatgtaaga
     4081 tttatttata ttatcaatac tttacttgga gtattatgtc tttagcagtt atctacttca
     4141 attgaattat ctaaaatata ataaatttgt tttgcaaatt gacgagaatg tttttctttt
     4201 tctttattca taaaattagg tttacctaaa tcacgttcaa caagcattcc tatatatttt
     4261 aactgtgaat gcttacagta gcgcttcata ccattttccc aaatatcagc acccttttca
     4321 ggtctatatc cacatgttgt aataagtgct actggttttc ctgcccatag agcggggcct
     4381 ttctcattac catagtactt attcattcca tacaccaatc tgtcaagtag tgctttcatt
     4441 ggagaagtgc agtaccaaga ataaattgga gtagcaaaaa caattaaatc acacataaga
     4501 attttcttga aaattttctg aacatcatct ttacaataac atccaaaatc aaataagtta
     4561 tcctgacatt ttttacaagc aatacatggc tctattttac aatcatataa ccatattact
     4621 tcaatatttg aattataatt ttctaattcc tcaataaaag gttttaaaat agcagcagta
     4681 ttaccttttt tcttaggact tcccataaga atgcatattt ccatattgat acctccacag
     4741 tattttaaaa tagtttttag tgcttaatat agttttagga tactatgaga atacgttaac
     4801 aacaatacac tttgtatatt tttaataaaa ttgaaacaaa ttgttttatc aataaaagtt
     4861 ggaatgtatc tataattaaa aatattttag gtatgttttt acatatatgt gtgtagttaa
     4921 ctatggtaat ttattgtaaa attatcttat aggattataa ttgcttaatt tatgattata
     4981 tttaaaatct tggaggtaaa aaatatgtac tcaaatgaag actataaatt tatagaagaa
     5041 aatgcaccat gttatatata tgatgagact aatataattg aaaaatgtaa ggaattgaaa
     5101 aataatattt taggttttga ttttttatat tctataaaaa caaatccata taacaaaatt
     5161 gtaaaaagca ttgcagagca aggttttgga gcagatgctt cttcaactaa tgaagttatg
     5221 atttcatcag aatgtggagt ttctaaatat gatatttatt attcatcacc aggcaaaaca
     5281 gagattgata tagaaactgt tttagataaa tgcataatta ttgcagatag tattacagaa
     5341 atagaactta taaataagat atctaaaaag cataatcaga aagttaaaat aggaataaga
     5401 ataaatcctg attttactat ggatgatgat agtgctaaat caagcaaatt tggtatagat
     5461 atagaagata tgagcagtgt ttttaagatg ttaagtatgt gtgattatgt atctgtagtt
     5521 gggattcata tacatattaa atctcaggta ttggattttg aaaagttagg acattattat
     5581 gaaaaatgtt tttttatagc tttaaattta aataaaagta agtatattaa tattgaattt
     5641 ataaattttg gtagtggact tggagtagct tatgataatc ttagagaaaa tccacttaat
     5701 cttaaagagc tttcaaaatt aactcaaaag attgttttta ctaattcaaa cacactgaaa
     5761 tcaagacttc ttattgaatc aggacgtttc atagtttgta aatcaggtaa ttattatgtt
     5821 aaaattgtag atattaaagt atcaagaggg aaaaaatatc ttattgtaaa aaatggactt
     5881 aatggattta tgagacctat tatttctaat ttaattgaaa atattacttg tggagtagaa
     5941 ttagaaggtc aagagccatt atatacatca aatcatgcct tttctctttc agtattaaat
     6001 aatttaaaag aagaagagta tgtaaatgtt gttgggaatc tctgtacatc attagatgta
     6061 attgcaaata atgttaaatt gaaaaaagca aatataggag atttgatttc aattagtaat
     6121 gctggaagtt actcctattc tttatcacca ctattatttt cgagttatga atttcctaaa
     6181 caatattata aaagaaaaga tggatatatt gagtaaacaa taggataaat taataaactt
     6241 agtattattg aaaaaattta aaatgcaggg ggtgtaattc tatatggaat tgtggaaaag
     6301 aaatttattt gtatgttgga taggtatgtt ttttagttct atagggatga gccaaatagc
     6361 acctatactt ccattatata ttaaacagtt gggggtaact gatgttagtt taatacaaca
     6421 gtattcaggt ataatttttg gatgtacctt tgtagtagca gcattctttt cgccaatttg
     6481 agggaaagct gctgataaat atggaagaaa atcaatgctt ttacgtgcaa gtattgggat
     6541 gggaatagtt atatttacca tggcgtttgt tcaaaatgtt tatcagttat taggattaag
     6601 aatacttcaa ggagttttta cgggttatgc tactgcatgt actactctta ttgcaacaca
     6661 gacagataaa aatcattcag gatgggcttt gggtactctt gcaactgcat caactactgg
     6721 ctcattaatt ggtccaacag taggaggata tatagaatca atcttgggtc ttaaaagtac
     6781 atttattata acaggaggat tgttgtttgt atcatttatc atctctattt tatttgtagt
     6841 agataatttt aaacctaaag aaataaaaga atcaatttct atacctaaag aacaattaaa
     6901 tatattgcca aataaatttt taattgcatc tatttttgta accacattca ttactcaact
     6961 agctttatac tctatagaac caattgtaac catatatata tcacaactta caaattttgc
     7021 atcaaatgta gctttaatag ctggattgac attttctgca tcaggattag caaatttatt
     7081 agcagctcaa aaacttggaa aaatgtcaga taaaataggt cctcaaaaag tattgttaat
     7141 ttcactttta tgggctggga ttatatttat accacaagct tttgtaaaaa ctgcatggca
     7201 actgatgttg ttgagatttt tattagggtt gtcagttgca gggcttaatc cttctgttaa
     7261 cagcttgctt aaaaaaattg caccagaaga atatgtggga aaaatatttg gatataatgc
     7321 atctgcccag tatataggtt gttcatcagg agcattttta gggggacaaa tatcagcaca
     7381 tttaggtatt agaacagtat ttttttcaac aagtttattg ttgtttatga atgcactttg
     7441 gatgtataaa tttactggat tgtttattaa agataagact aaataatcat actatttaca
     7501 tttctaattt tatgatgtaa aataaatatt ttttatgtaa tttatcatag aattatataa
     7561 aaaataactc tatggtaaaa attggaaaat attattatcg acaaaatctt tgacttaaaa
     7621 actatattgc gatagtatta atttaataaa agtttgagag gggcgtaagg ttatgacaat
     7681 ttcaaaaata ccacaagcag aattaaaagt tatgaagttt atttgggaaa aaaatgatac
     7741 agtaacatca aaagaaacta ttgaagctat ggaacgaaaa tatggttgga aacaaacaac
     7801 tacattgaca cttttatcaa gacttgttaa aaaacatttt ttaagtgcag aaaaaataga
     7861 tagatacact cattatacaa tagttgttgg acataaggag tatttaagtg ttgagacaaa
     7921 agatttttta agcaatatac acgacaattc tttacagagt ttaatttcag cactacatga
     7981 tgatgaagtt gttgataaag atatgttgga tttatttgaa agtcatttta aaaatttaga
     8041 agaagagtaa ttatatcagg ataaaactct aagaatttaa ttaatctatg gctgaaaacc
     8101 ataaatggta ttatttaatc agctactcac tattcataaa gtaatattaa ataggttctc
     8161 taattagtaa aatacctaaa ataattggaa tagggagtat caagttaaat gataccactt
     8221 atttaaataa gaaatgattt ttaaaattta aaataattt
//
LOCUS       DAMJWP010000159         8247 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.164, whole genome shotgun sequence.
ACCESSION   DAMJWP010000159 DAMJWP010000000
VERSION     DAMJWP010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8247
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.164"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            101..1132
                     /locus_tag="PC700_002744"
     CDS             101..1132
                     /locus_tag="PC700_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDF2582040.1"
                     /translation="MENICDMNNKVKVAVLDTGIDKEHDYLKDNLVGGIAFECMHDYI
                     FISDKFDDEDGHGTACASIIKKEYEDVELFVIKILRKDGITNIKVLEEALRYLLDTNI
                     RLINLSLSVIGVESVKGLFEVCYELFRKGKIIVCSLANDFDLSYPAMFNNVIGVRGFT
                     LDGDDSFWYNKKYDVQCVMDSNSYISCDINNSYRLPPKCNSYLSAKLTGKIAKILSEK
                     PGITISDLNDKLESLATRNHWDSCDFDECSRIVNFKLDLYDKENALLMKVADVVRDCL
                     DIEDNNEKLFKGSLFNKEIGLVYDNCFNLLKKLEHRFDIKFNYMDISKYDLVSIYTLT
                     ELVERYKNL"
     gene            1265..2563
                     /locus_tag="PC700_002745"
     CDS             1265..2563
                     /locus_tag="PC700_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDF2582041.1"
                     /translation="MKYEEALEYISQTNKFGIRLGLENIGKLLELLGNPQETLNIIHV
                     AGTNGKGSVCSFVSNILRECGYKVGLYTSPYLETFTERIRVNGQNIPQDDVARIIELI
                     KEKIEIMVKEGYAYPTEFEVVTAMAFYYYSEQKVDFVALEVGLGGRYDATNIITKSLV
                     SVIVSISLDHTGILGDTIEKIAYEKAGIIKENGVVLVYDQTDEAKDVIKSVCKEKKAK
                     YIEVDFDDINIKKSDINSQIYDCTVMKETYRDLEIKLIGEHQINNSILAISVIKYLKD
                     INKLANINEESIRKGLINTKWPGRIEKIKENPIFIIDGAHNEDGAKSLAKALDKNFKG
                     RKLTLLIGMLEDKDIDSVLEILLPHFNKVITTTPNNPRAINSDILREKVLKYVDDVTS
                     KHEIEDAVNYTLETSSEDDIIISAGSLYMIGTVRTLVKKL"
     gene            complement(2677..2922)
                     /locus_tag="PC700_002746"
     CDS             complement(2677..2922)
                     /locus_tag="PC700_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HDF2582042.1"
                     /translation="MKVTFNPSGVCCREMIFEVDENNVIVDAEFIGGCNGNLSGLRSL
                     IIGQNALEVADKLNGIDCGGKGTSCPDQLSKAIRQSI"
     gene            3070..3936
                     /locus_tag="PC700_002747"
     CDS             3070..3936
                     /locus_tag="PC700_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HDF2582043.1"
                     /translation="MKIYISNYLSKSISIVDYSTLELEKEIVLEDNIYPHHFCIEKEK
                     NLIYIPSSSNGILYVLDLSNDKIIDTVSIGGSLSQVVLSDNELFVANEDSNSIYVLDK
                     NTLNPIGIIGVDNMPHGFDFDRESKKLYVPCINSIVCIDTINKNIQKKINMDFRAWHI
                     ELDKQKKEMYISTLDGKLIIIDEVSMDIKKVLYEFLLPVEIRFNYSGKKVYVADLGYN
                     NVRILDYTTSRYIGNIEINGIPQGLEISKDEKLLFVSDTQENSVKVYETANNKLIKEI
                     KVGKEPTTIICL"
     gene            complement(3940..4476)
                     /locus_tag="PC700_002748"
     CDS             complement(3940..4476)
                     /locus_tag="PC700_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HDF2582044.1"
                     /translation="MFENSSEELAYHKLLILYILEKIKMDLTNSQITQVVLETEMMNY
                     FSLQQLLSQLMESKFLTTYKDSDREYYALTQRGVESLEYFFNRIPTSVTEKIDKYIDS
                     NKENLLADTQVKSSFVKQSDNEFIVNLRVIENQSNLIDLNLNVSSEKQAKLICNNWKN
                     NASYMYAEVIDLLIRDIH"
     gene            complement(4666..4869)
                     /locus_tag="PC700_002749"
     CDS             complement(4666..4869)
                     /locus_tag="PC700_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HDF2582045.1"
                     /translation="MSNSNRTVVPEAKAALNQMKLEIANEIGLSNYENIDKGNLTARE
                     NGYVGGYMTKKLVEMAERQMAGK"
     gene            5160..5924
                     /locus_tag="PC700_002750"
     CDS             5160..5924
                     /locus_tag="PC700_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2582046.1"
                     /translation="MKREVKRTKALIYSVLFFSLLLVLIFVFVYGIKYICSKYDNSKM
                     PIYKVDTNEKKIALSFDVAWGTDNMDDILKILDKHNVKATFFLVGSWVDDNEEIVKAI
                     DEKGHEIGNHSNTHANLKEISKEDITKEIETTSEKIYNITGKKTNLFRPPFGDVNNKA
                     MDICSDLGYKVIKWDVDSIDWKELGPNHVIEKVIKESQPGSIVLFHANINDVDNYLDT
                     IISRLKKDGYSLVKISDLLYKDNYVVDSNGVQKTKN"
     gene            6313..7626
                     /gene="prdC"
                     /locus_tag="PC700_002751"
     CDS             6313..7626
                     /gene="prdC"
                     /locus_tag="PC700_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase-associated electron transfer
                     protein PrdC"
                     /protein_id="HDF2582047.1"
                     /translation="MNKLYSILLKQHVGGPDKPVVSVGDVVKKGTLIAEPTGLGANIY
                     ASVSGKISEINDQAIVIEADEVQEDTFEPLKGEGILDLIKEAGVVGMGGAGFPTHVKL
                     NIDLNGGTILANAAECEPLLAHNIKEIEERPEIVYQGIKYAMEVTNAGKGMLAIKSKH
                     PKAIEAFKKVIKPGDNIEVAELVDMYPMGEERAIVRDVLGKLLEPTQLPSEANAVVIN
                     VETLTRIVEAVEQKRPVISKNITVVGQLNSGKESIVFEDVPIGTTVGELIERAGGIKG
                     EYGEIILGGPFTGKATTLDAPITKTSGGIIVTMPFVNEKRKMGLLVCACGPNEERMRD
                     IATKMGVTDIVSVQKCKQAQEIKGALKCENPGHCPGQAQKCIEFKKAGAEVILIGNCT
                     DCSNTVMGSAPKLKLGVYHITDHVMRTVNHPLIRRIKGDVRLKIK"
     gene            7795..7957
                     /locus_tag="PC700_002752"
                     /pseudo
     CDS             7795..7957
                     /locus_tag="PC700_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011020315.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3320 a          852 c         1394 g         2681 t
ORIGIN      
        1 atgtttttag caagaaatat agcatttaaa agataaattg tgcaacttaa atcaaagttt
       61 aagatgaact attaacatag atatattaaa aacttttgta atggagaata tatgtgatat
      121 gaataataaa gttaaggttg cagtattaga tacaggaata gacaaagaac atgattactt
      181 aaaagataat ttagttggag gaattgcttt tgaatgtatg catgattata tctttatatc
      241 agataaattt gatgatgaag atggtcatgg aactgcttgt gcatcaataa taaagaaaga
      301 gtatgaagat gtagagttat ttgtaataaa aattttgaga aaagatggta ttacaaatat
      361 aaaagttctt gaagaagcat taagatacct tttagacact aacataagat taattaattt
      421 aagtttatca gtcataggag tagagagtgt taagggctta tttgaagttt gttacgaatt
      481 gtttagaaaa ggaaaaataa tagtatgttc actcgctaat gattttgatt taagttatcc
      541 agctatgttt aacaatgtta taggtgtaag aggatttact ttagatggag atgattcatt
      601 ttggtacaac aaaaaatatg atgttcaatg tgttatggat agcaattcat atataagttg
      661 tgatataaat aattcctata gattaccacc aaaatgtaat agttatttat cagcaaagct
      721 tactggtaaa atagctaaga tattatcaga aaaaccaggc ataacaatat ctgatttaaa
      781 tgataagctt gaatcattag caactagaaa tcattgggat agttgtgatt ttgatgagtg
      841 tagtagaatt gtaaacttta aattggattt gtatgacaaa gaaaatgctc ttcttatgaa
      901 agttgctgac gttgttaggg attgtttaga tattgaagat aataatgaaa aactctttaa
      961 gggtagcctt tttaataagg agataggttt ggtttatgat aattgtttta atctgttgaa
     1021 aaagctagag catagatttg acataaagtt taattatatg gacatctcaa aatatgactt
     1081 agtttcaata tatacactga ctgaattagt ggaaagatat aaaaacttat aaataaagat
     1141 ttgtagctaa atttaatgta gaaggtatta aattatatgt attctttgtc ggtagattaa
     1201 aagtaaaaaa tatactataa ttagatagca taagagaaaa tgccaataca gggggagtta
     1261 gataatgaaa tatgaagaag cattagagta catatcacaa actaataaat tcggtataag
     1321 attaggatta gaaaatatcg gaaagttatt agaattatta ggaaatccac aagaaacttt
     1381 gaatataata catgtagctg gaactaatgg aaaaggttct gtgtgttctt ttgtttctaa
     1441 tatactaaga gagtgtggat ataaagttgg tttatataca tctccatatt tagaaacttt
     1501 tactgagcgt ataagagtaa atggacaaaa tataccacag gatgatgttg ctagaataat
     1561 tgagttaata aaagaaaaaa tagagattat ggtaaaagaa ggctatgcat atccaacaga
     1621 atttgaagtg gttactgcaa tggcatttta ttactattca gaacaaaagg tagactttgt
     1681 ggcattagaa gttggtttag gtggcagata tgatgctaca aatataataa caaaatcact
     1741 tgtaagtgtg atagtatcta taagtttgga ccatacaggt atacttggag atacaataga
     1801 aaaaatagcg tacgaaaagg ctggaataat aaaggaaaat ggagtagtac tagtatatga
     1861 ccagactgat gaagcgaagg atgttataaa gtcagtttgt aaagagaaaa aagctaaata
     1921 tatagaagtt gattttgatg atataaacat aaaaaaatca gatataaatt ctcaaatata
     1981 cgattgtact gtaatgaaag aaacttatag agaccttgaa ataaaattga taggtgaaca
     2041 tcaaataaac aattctatct tggcaattag tgtaataaaa tatctaaaag acataaataa
     2101 attagcaaat ataaatgaag aatctataag aaaaggactt ataaatacta aatggccagg
     2161 aagaatagaa aagataaaag aaaatccaat ttttataata gatggtgcac ataatgaaga
     2221 tggagcaaaa tctttggcaa aagcacttga taagaatttt aaaggtagaa aattaacctt
     2281 attaataggt atgttagaag ataaagatat tgatagtgtt ttagagatat tgttgccaca
     2341 ttttaataag gtaataacaa caacaccaaa taacccaagg gccataaatt cagatatatt
     2401 aagagaaaaa gtattaaaat atgtagatga tgtaacatca aagcatgaga tagaggatgc
     2461 agtaaattat acattagaaa catcaagtga ggatgatatt ataataagtg caggttcatt
     2521 atatatgatt ggtacagtga gaactttagt aaagaaatta taattttata aaataaatat
     2581 acaaataaag atataaaaaa gagttgtcta aaaaatggac aactcttttt atataaagta
     2641 agtttttaat aattttactg atgtttatat aagtaattat atagattgac gtatagcctt
     2701 agataattgg tctgggcaag aagtaccttt tccaccacag tcaataccgt ttaatttatc
     2761 agcaacttca agagcatttt ggccgattat taagcttcta agtcctgata aattaccatt
     2821 acatcctcct ataaactcag catcaacaat cacattattt tcatcaactt caaaaatcat
     2881 ttctctacaa catacaccac ttggattaaa tgttactttc attaaaaaga cctccattaa
     2941 atattatttc tttttttaac aataataata ataccaaata tatgcgtaat aatgcaacta
     3001 taaagcataa tacaagctaa tgaaataata tattattgta gtatatgtat tgtagagagg
     3061 aggggttata tgaaaatcta tatttctaac tacttatcaa agagtattag catagttgat
     3121 tattctacac ttgaactaga aaaagaaata gtattagaag ataatatata tccacaccat
     3181 ttttgcatag aaaaagaaaa aaatttaata tacataccta gttcaagtaa tgggatactt
     3241 tatgtcttag atttaagtaa tgataaaata attgatactg tctctatagg aggaagtctt
     3301 agtcaagttg tacttagtga taatgaattg tttgtggcaa atgaagattc taatagtata
     3361 tatgtactag ataaaaacac attaaatcca ataggaatta ttggagttga taatatgcca
     3421 catggatttg actttgatag agaaagtaaa aaattatatg taccgtgtat aaactcgata
     3481 gtatgtatag atacaattaa taaaaatata caaaagaaaa taaatatgga ctttagagct
     3541 tggcatatag agcttgataa acaaaagaaa gaaatgtata tatcaacact tgatggaaag
     3601 cttataataa tagatgaagt aagcatggat ataaaaaagg tattatatga atttttatta
     3661 ccagtagaaa taagatttaa ttatagtggc aaaaaagttt atgttgctga tttgggttat
     3721 aataatgtaa gaattttaga ttatacaacg agtagataca ttggaaatat tgaaataaat
     3781 gggattcctc aaggcttaga aatatctaaa gatgaaaagc tactatttgt atcagataca
     3841 caagaaaatt cagtaaaagt ttatgaaact gcaaacaata aattaataaa agaaatcaag
     3901 gtaggaaaag agcctacaac tataatttgt ttgtaggctt taatgtatgt ctcttataag
     3961 taaatcgata acttcggcat acatataaga tgcattattt ttccagttat tgcaaataag
     4021 ttttgcttgt ttctctgatg aaacatttag atttaaatct attaagttag actgattttc
     4081 tatgactcta aggttgacaa taaattcatt atcactttgt tttacaaaac tagactttac
     4141 ttgagtatct gctagtagat tttctttgtt agaatctata tacttgtcta ttttttcagt
     4201 aacactagta ggtattcgat taaagaagta ttctagactt tctacgcctc tttgagtaag
     4261 agcataatat tctctgtcag agtctttata tgtagtcaaa aacttagatt ccataagctg
     4321 agataaaagc tgttgtaatg aaaaataatt catcatctca gtctctaata ctacttgagt
     4381 tatttgagaa ttagttaaat ccattttaat tttttctaat atatataaaa ttaaaagttt
     4441 gtgataggct aattcttcag acgagttttc aaacataaat atcatcctcc gtcaaactat
     4501 ttctatatat aatatattaa actaatttgt acaaaattaa aagaacaatt tgtattgtat
     4561 tattaatctt taataattca tataaaacaa ttttataaat aaatatagag aaacagggct
     4621 gctaattagc agccctgttg atacccaggt gtcaaaattt actatttatt ttccagccat
     4681 ttgtctttca gccatttcaa ctaatttctt agtcatatat ccaccaacat atccattttc
     4741 tctagctgtt aagttacctt tatctatgtt ttcatagtta gataaaccta tttcgttagc
     4801 tatttctaat ttcatttggt ttaaagcagc ttttgcttct ggaactactg ttctgtttga
     4861 atttgacata gtaaatttcc tcctcaaata attagtttat gatgtctaac aacactttta
     4921 tttatgtgat gttaatacta atttctccaa aataaaaata atatatacag aaaaataaag
     4981 gtaaaaatgt aaaaaatata aaaaaattaa ataaatgagt attttagaga gtttaattag
     5041 gtcaatatag gttttatttt tttagttatt gtcttataaa ttaaatatta cagtactttt
     5101 ttgagctgaa aaaaaataaa ttttaaatat agaagaaatt atttcaaagg gggaatagta
     5161 tgaaaagaga agttaaaagg actaaagctt tgatatatag tgttttattt ttttcattat
     5221 tattagtgtt aatatttgtt tttgtttatg gaataaaata catatgttct aaatacgaca
     5281 attcaaaaat gcctatatat aaagttgata caaatgagaa aaaaatagcc ttgagttttg
     5341 atgtagcatg gggaactgat aatatggatg atattttaaa aattttagat aaacataatg
     5401 taaaagctac cttcttttta gttggtagtt gggtggatga taatgaagaa attgtaaaag
     5461 ctatagatga aaaaggacat gaaattggaa atcattcaaa cactcatgcc aatttgaagg
     5521 aaatatcaaa agaagacata acaaaagaga tagaaactac atcagaaaaa atatataata
     5581 taacaggaaa aaagactaat ttatttagac ctccttttgg ggacgtaaac aataaagcta
     5641 tggacatttg tagtgattta ggttataaag taattaagtg ggatgtagat tctatagatt
     5701 ggaaggagct tggaccaaat catgtaattg agaaagtaat aaaagaatct caacctggtt
     5761 caatagtttt attccatgct aatataaatg atgtagataa ctatttggat actataataa
     5821 gtcgactaaa aaaagatgga tacagtctag tcaaaatatc tgatttgttg tataaagata
     5881 attatgtagt tgattccaat ggagtgcaaa aaactaagaa ttaataagta ataaatgtat
     5941 attttaagta aagttgtgat aaattatata gtatatgtta gaaaagtgtc gataaaaaga
     6001 cggtgtctat tttttgacac tttttataca tgtatgcaat atcaataatt atagattaaa
     6061 ttgattaaaa aagtgtctaa taattgactt ttttttgatg gatttaaaaa tttagaagaa
     6121 ttaatgaatg ctgtaagaca gtatttccaa agggtataag tttgtttaaa agttggcata
     6181 gaaattgctt tgttaataag cgatatgttc tatagaaata aacaatatgt taagacctta
     6241 taatagaaaa atagcattaa cattatttga acagcttata aaaaaattaa tatacttaag
     6301 ggggcgtact agttgaacaa actatattct attttattaa aacaacacgt tggtggtcca
     6361 gataagccag tagtaagtgt tggagatgta gtaaaaaaag gaactctaat cgcagaacca
     6421 actggattag gggcaaatat ttatgccagt gttagtggaa aaataagtga aataaatgac
     6481 caagcgatag taattgaagc tgatgaagta caagaagata cttttgaacc attaaaagga
     6541 gaaggtatac ttgacttaat taaagaagct ggagtagtag gaatgggtgg agcaggattc
     6601 cctactcatg taaaattaaa catagattta aatggtggaa caatattagc aaatgcagct
     6661 gaatgtgaac ctttattagc tcataatata aaagaaatag aagaaagacc agaaatagtt
     6721 tatcaaggta taaaatatgc tatggaagtt actaatgctg gaaaaggtat gctagcaata
     6781 aaaagcaagc atccaaaagc aatagaagct ttcaaaaaag taataaagcc aggagacaac
     6841 atagaagttg cagaactagt tgatatgtac ccaatgggag aagagagagc aatagtaaga
     6901 gacgttctag gaaaattact tgagccaact caattacctt cagaggctaa tgcagtagta
     6961 ataaacgtag aaacactaac tagaatagta gaggctgttg agcaaaaaag accagttata
     7021 tctaaaaata taactgtagt tggtcaatta aacagtggaa aagagtctat agtatttgaa
     7081 gatgtgccaa ttggaacaac tgtcggagaa ttaattgaaa gagctggtgg aataaaaggt
     7141 gaatatggtg aaataatcct aggaggacct ttcactggta aagctactac tttagatgct
     7201 ccaataacta aaactagtgg tggtataata gttactatgc catttgttaa tgaaaagaga
     7261 aaaatgggtc ttttagtctg tgcatgtgga cctaatgaag agagaatgag agatatagca
     7321 actaaaatgg gagttactga tatagtatca gttcaaaaat gtaaacaagc tcaagaaatt
     7381 aaaggtgcat taaaatgtga aaatccagga cactgtccag gacaagctca aaaatgtatt
     7441 gaatttaaga aagctggagc agaagttata ttaataggaa actgtactga ctgtagtaac
     7501 acagtaatgg gttctgcacc aaaattaaag ttaggtgtat atcatattac tgaccatgta
     7561 atgagaacag ttaatcaccc attaataaga agaataaaag gtgacgtaag attaaaaata
     7621 aaataataga taaatagaag ttagacatct ttagaagtaa aacttaaaaa ttacttctaa
     7681 ggatgttttt taatgtattt tgagggcaat atatagcttt aaaagaaatc aaaaaagtgc
     7741 caaatgtaaa actaattaaa ccatatatat ttaagaaggg attgaattgg agttgtgaat
     7801 tatgttagta gaaaccattc taagtgtttg ttattggtac atttgatatt tgtatgtaaa
     7861 tacagaaaaa actttttgct cagatggata ctttgctttc agtgtaggaa atgtttctaa
     7921 agaaatgatt gaaaaataca tacaaagtaa gggataggtt tacatccacc aacctaaagg
     7981 tatagtggtt ttacgcctta atttataaaa agaaattaat ttatattatt attaagttta
     8041 aaataaaaag tctcaaattg atttataagt cattttggga cttttttatg taaatataat
     8101 atgcaaatat agtttgttta aaaatatcta ttttaattat attcttaaat ttatgattgg
     8161 atacaggcat tcattaattg tttgatgaat agaaaaatgt tttgaaggtg aaaatagtat
     8221 tgaaaaaata atatactaag ggtataa
//
LOCUS       DAMJWP010000160         8157 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.166, whole genome shotgun sequence.
ACCESSION   DAMJWP010000160 DAMJWP010000000
VERSION     DAMJWP010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8157
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.166"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(67..1707)
                     /gene="bclA2"
                     /locus_tag="PC700_002753"
     CDS             complement(67..1707)
                     /gene="bclA2"
                     /locus_tag="PC700_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA2"
                     /protein_id="HDF2582048.1"
                     /translation="MSDISGPSLYQDVGPTGPTGATGPTGPTGPRGATGATGANGITG
                     PTGNTGATGANGITGPTGNMGATGANGTTGSTGPTGNTGATGANGITGPTGATGATGA
                     NGITGPTGNKGATGANGITGPTGATGATGANGITGPTGNTGATGANGATGLTGATGAT
                     GANGITGPTGATGATGANGVTGATGPTGNTGATGPTGSIGATGANGVTGATGPIGATG
                     PTGAVGATGPDGLVGPTGPTGPTGATGANGLVGPTGPTGATGANGLVGPTGATGATGV
                     AGAIGPTGAVGATGPTGADGAVGPTGATGATGANGATGPTGAVGATGANGVAGPIGPT
                     GPTGANGVAGATGATGATGANGATGPTGAVGATGANGVAGPIGPTGPTGANGTTGATG
                     ATGATGANGATGPTGATGATGVLAANNAQFTVSSSSLGNNTLVTFNSSFINGTNITFP
                     TSSTINLAVGGIYNVSFGIRAILSLAGFMSITTNFNGVAQNNFIAKAVNTLTSSDVSV
                     SLSFLVDARAAAVTLSFTFGSGTTGTSPAGYVSVYRIQ"
     gene            complement(2085..2624)
                     /gene="hpt"
                     /locus_tag="PC700_002754"
     CDS             complement(2085..2624)
                     /gene="hpt"
                     /locus_tag="PC700_002754"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDF2582049.1"
                     /translation="MDIETKKWEVLYSEEKIKDKLRELGAIIEKDYKDKNLMVVSLLK
                     GSFIFCADLVRNINLPLRVNFMTTSSYGNNEESTGKVKVVSDVTTDIAGYDVLVVDDI
                     TDSALTMDFVLKHLKAKNPASLKCCVLLDKPSRRKVDLVPDYCGFEIEDKFVVGYGFD
                     FGDYYRNVPYIFNVTDEDR"
     gene            complement(2952..4259)
                     /locus_tag="PC700_002755"
     CDS             complement(2952..4259)
                     /locus_tag="PC700_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HDF2582050.1"
                     /translation="MRDIRKELVEMLKAEVKPAVGCTEPVALALACAKAKELLGEEIV
                     ENRMLVSPSIYKNGMCVGIPGTERLGLKIAAALGIVGGHSENGLSVLETLTKEEVKIA
                     EDYMDNTPLSITPADTREKVFIEVVLKGKNHIAKVRIRTKHDNFTFLEKDGEVLLDNE
                     PKVSASNDAAEKAESLMDTVTIQELIKNVEEIDFKDIEFLLDGVKMNEEMAEYGLKQK
                     TGIGVGYGIKKSIEEGLLGNDVINYAMMLTAGASDARMAGVKMPVMSSNGSGNHGLTA
                     ILPIVAYNKKFPQSDEKLAKALAISHLVTGYIKNYTGRLSAVCGCGVAASTGATAGIS
                     WLMNGTEKQIEGAIENMIADLSGMICDGAKAGCALKLSSAASAAIQSAIIAKQDCFVP
                     PLNGIVGSSVEQSIQNLGRVSDKGMSITDEIILNVMNDMNKVD"
     gene            complement(4684..6459)
                     /locus_tag="PC700_002756"
     CDS             complement(4684..6459)
                     /locus_tag="PC700_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2582051.1"
                     /translation="MMVMIGLEYIKSDIQNIAEAIVSVLNIDVTIVNKELFRIAGTGV
                     YIDKIGEKVDKYTAFKKSLTEQITILIDDPKSSDVCRECYKSGACVEFAEVCCPIICD
                     GDSHGVIGLIAFTKEQAQIIENNKSGLINFLGKMADLISNKLKAQIKTYELELEKKKL
                     ETLLNNMDKAVVSIDVDGKIDKYNSKFKELFNIKDSITGKNIFVELDFIKKPSINNFK
                     KDKSCSFFYKRYGYDSKGIYNINKIVLKNELKGYVIDFIDKRDAIKNYNKMNKDYRIK
                     LENIIGESVAIRNSKKEALIASKSTSTVLITGESGTGKELFARAIHNHSNRVDNPFIA
                     VNCAAIPDNLLESELFGYEEGAFTGAKKGGKLGMFEVAHKGSIFLDEIGDMSLHLQSK
                     LLRVLQEKELNKIGSKSNVSIDVRIIAATNKDLEKMVQNGTFREDLYYRLNVIPIKLP
                     SLRERKEDIPLIIKHMVKEYSKKLDKNVIDIDSRVVDAMMEYKWPGNIRELQNIIEYS
                     VNMSSSSIITMNVIPQKIKNTSVEELEVKSDRIIPLDELEKIEIIKALNKYKDYKKDK
                     ELTAKALGISRATLYRKIEKYNIIS"
     gene            complement(6456..6593)
                     /locus_tag="PC700_002757"
     CDS             complement(6456..6593)
                     /locus_tag="PC700_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582052.1"
                     /translation="MEINYELIIIVMLFVILMSIQFTLNKIYLILKEIRQILMLKKNK
                     E"
     gene            complement(6671..7240)
                     /locus_tag="PC700_002758"
     CDS             complement(6671..7240)
                     /locus_tag="PC700_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF2582053.1"
                     /translation="MKSILCQINKQPVKAKYTSDELECFLDTIEYNVGDKILLVGHIG
                     NLGKRLRGLGTSVTILENSNYEDVCYSLVYNENCNVVKGKLEFLPFDDNQFNKIIIVD
                     QFNVITDCEKASSEIQRVLKGNGEVILEDLNLKNLKVKLNYLKHKFCGSVAKIHYPHE
                     VFNIFSRLNFDGMIKESNKLKYIYVGKRR"
     gene            complement(7424..8059)
                     /locus_tag="PC700_002759"
     CDS             complement(7424..8059)
                     /locus_tag="PC700_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF2582054.1"
                     /translation="MIAVKDENNVVNLRGELDNKLEFSHEIFGEKFYNMKIKINRLSD
                     SFDILPMTVSERLLQDIDLDKQNLVNVVGQLRSYNKTLNNKNRLVLTVFVREIKSIDE
                     ENKDPNSIFLDGYVCKEPVYRKTPLGREITDLLVAINRPYNKSDYIPSIVWGRNAKFA
                     KNLKVGDRIQLWGRVQSREYEKKIDENNVVKKMAYEVSISKIKKLDENNNE"
BASE COUNT         2320 a         1642 c          951 g         3244 t
ORIGIN      
        1 attatcctat ttattggtat atgaaatcat attgtttatt attttataat cttttcatca
       61 tataaactat tgtattctat aaactgatac atacccagct ggagaagtac ctgtcgtgcc
      121 tgaaccaaat gtaaagctta aagtaacagc tgctgctcta gcatcaacta aaaagcttaa
      181 acttacactt acatctgatg aagtaagcgt atttactgct tttgcaataa agttattttg
      241 ggctactcca ttaaagttag tagtaattga cataaatcct gcaagtgaaa gtatggcacg
      301 tataccgaaa gatacattgt atatccctcc aactgcaaga tttatagtac tacttgttgg
      361 aaaagttata ttagttccat ttataaatga tgaattaaat gtcactaatg tattattccc
      421 taaacttgaa gaagatactg taaattgtgc attgtttgct gctaacactc ctgttgctcc
      481 ggtcgctcct gttggacctg ttgctccatt tgctcccgtt gctccggtcg cccctgttgc
      541 tccggtcgtt ccatttgctc ctgttggtcc tgttggacct attggacctg ctactccatt
      601 tgctcccgtt gctcctactg ctcctgttgg gcctgttgct ccatttgccc ctgttgctcc
      661 ggtcgctcct gttgctcctg ctactccatt tgctccggtt ggacctgttg gacctattgg
      721 acctgctact ccattcgctc cagttgctcc tactgctccc gttgggcctg ttgctccatt
      781 tgcccctgtt gctccggttg ctccggttgg acctactgct ccatctgctc ccgttgggcc
      841 tgtcgctcct actgctccgg ttggacctat tgccccagct actcctgttg ctccggtcgc
      901 tcctgttgga cctaccaaac catttgctcc ggttgctccg gttgggcctg ttggacctac
      961 caaaccattt gctccggttg ctccggttgg gcctgttggg cctgttggac ctaccaaacc
     1021 atctggacct gttgctccta ctgctccggt tggacctgtt gctcctattg gacctgtggc
     1081 acctgttact ccatttgctc ccgttgctcc tatacttccg gttggacctg ttgctcctgt
     1141 atttcctgtt gggcctgtag cacctgttac tccatttgct cccgttgctc ctgttgctcc
     1201 tgttggacct gttatcccat tcgctcccgt tgccccagtt gctccggtta gtccggttgc
     1261 accatttgct cccgttgctc ctgtatttcc tgttggacct gttattccat ttgctcccgt
     1321 tgctcctgtt gctcctgttg gacctgttat cccattcgct cccgttgctc ccttgtttcc
     1381 ggttggacct gttattccat ttgctcccgt tgctcctgtt gctcctgttg gacctgttat
     1441 tccatttgct ccagtcgctc ctgtatttcc tgttggtcct gtagaacctg ttgttccatt
     1501 tgctccagtc gctcccatat ttcctgttgg tcctgttatt ccattcgccc cggttgctcc
     1561 cgtatttcct gttggtcctg ttattccatt tgctccggtc gctccggttg cgcctctagg
     1621 ccccgtcggt cctgttggac cagtagcacc tgttggccct gttggaccta catcttgata
     1681 taaacttgga cctgaaatat cactcatata attaatcctc cttttttaaa gttagagtat
     1741 tactctataa atattgagta ttactctata taattatatg aatcgacaat ttcttatgtg
     1801 ataatgtatc tatttgctta tatcaacata tatgctttga ttcttaaatt tcattacttt
     1861 tttgtaaaat cttttacaat tattagtact cttttacaat tattaataca attaaaaaac
     1921 tcatttccca tcaattattt aaaatttttt tattatgtta taaaaaatag ataattaaat
     1981 tgcataaaat taatttaata ctactttaat tactttttaa ataaaaaaac ctcaacacga
     2041 ttaataaaaa tcatattaag gcatccacaa aattatataa ttgcttatct atcttcatca
     2101 gtaacattga atatataagg aacatttcta taataatcac caaaatcaaa cccatatcca
     2161 accacaaact tatcttctat ttcaaatcca caataatctg gcactaaatc aacttttctt
     2221 ctgcttggtt tatccaaaag aacacagcat tttaaactag caggattttt agcttttaga
     2281 tgttttaaca caaaatccat agtaagtgca gaatcagtaa tatcgtctac aaccaaaaca
     2341 tcatatcctg ctatatcagt agtcacatct gaaaccactt taacctttcc agtactttct
     2401 tcattgtttc cataactaga tgtagtcata aaattaactc taagtggtaa atttatattt
     2461 ctaactaagt ctgcacaaaa tataaagcta ccttttaata gagaaactac cataagattt
     2521 ttatctttat aatctttttc tataattgct cctaattccc ttaatttatc tttaattttt
     2581 tcttctgaat acaatacttc ccattttttt gtttctatat ccataaaaag acctccaatt
     2641 gtaataatcc ttcttaattt tataagagat tgctcacttt ttcaatactc ttattcaata
     2701 ttagtcaata ttattgagtt tttatgttca ttcaacaatt attaacaatg tatcttatat
     2761 tttaagtagt tcatatatcc acactaaatt acatactata tattttagac aaaaaaagaa
     2821 aaaacttgca ctaacaagtt ttttctatta tacataaatt tatacataat catattttat
     2881 tagagggtac taattaaatt tatttatgct atttttgtta tacctcataa tacaaaacat
     2941 attataaagt actagtcaac tttattcata tcattcatta catttaagat tatctcatct
     3001 gttatagaca tacctttgtc acttactcta cctaagtttt gtatactttg ctcaacactt
     3061 gaaccaacta taccatttag tggtggtaca aaacaatctt gcttagctat tatagcactt
     3121 tgtatagctg cagatgctgc agaagataat tttaatgcac aaccagcttt cgcaccgtca
     3181 catatcattc cacttaagtc agctatcata ttttctatag ctccttctat ttgcttttca
     3241 gtaccattca taagccaaga gataccagca gttgctccag tagaagcagc aacaccacat
     3301 ccacacacag cagataatct tcctgtatag ttcttaatat aaccagttac tagatgagat
     3361 atagctaaag cctttgctag tttttcatca gattgtggga attttttatt ataagcaacg
     3421 attggaagaa ttgctgttaa tccatgattt ccacttccat ttgaactcat aacaggcatt
     3481 tttacgcctg ccattcttgc atctgaagct ccagcagtta acatcattgc ataatttata
     3541 acatcattac ctaaaagacc ttcttctata gactttttaa ttccatatcc gacaccaata
     3601 ccagtttttt gttttaatcc atattcagcc atctcttcat tcatttttac tccatctagt
     3661 aaaaattcta tatctttaaa atcaatttct tctacatttt ttattaattc ttgtatagtt
     3721 actgtatcca ttaaactttc agctttttct gccgcatcat ttgaagctga tacttttggt
     3781 tcattgtcta ataatacttc tccatctttt tctaagaacg taaaattatc atgtttagtt
     3841 cttattctca ctttagcaat atgatttttt ccttttaaca caacttctat aaatactttc
     3901 tctcttgtat cagcaggtgt tattgatagt ggagtattat ccatataatc ctcagctatt
     3961 ttaacttctt cttttgttag agtttctaac acacttaatc cattttcaga atggccacct
     4021 actataccta gagctgctgc aatttttaat cctaatcttt cagtacctgg tatacctacg
     4081 cacatgccat ttttgtagat actaggacta actaacatac gattttcaac tatttcttct
     4141 cctaataatt cctttgcctt tgcacatgct agagctaagg ctactggttc agtacaccct
     4201 actgctggtt taacttctgc ctttaacatt tccactaatt cttttcttat atctctcatt
     4261 atatataaac ctcccaatta agtttagatt tagtactcaa ataattaatt aaataatttc
     4321 ttatctatat ttatagcaaa atacatgcca tattttttat atctttttat atttttagtc
     4381 ttatattcta taaccattgg tatgactgct tctatttatg tatttttttt atttacatag
     4441 agtatttata ttctttaaaa tggagaaaca tatgacaatt tcgcaaaaag acaaaaaaaa
     4501 gtctcagaat gagattctat atcattttga gacggctcaa atgtgttatt ttttaactta
     4561 taaaaaccat tttttaattc ataaaaaaag attatgcaaa acacttatag tataagttta
     4621 aagctttctt taaagttatg aatttagaaa aatttaaatt ttatatagct ttttattata
     4681 ttactatgaa attatattgt atttttcaat ttttctatat aatgtagctc ttgatattcc
     4741 aagtgcttta gctgttaatt ctttatcttt tttataatct ttgtacttat ttaaagcttt
     4801 tattatctca atcttctcta gttcatcaag tggaattatc ctatcgcttt taacttctaa
     4861 ctcttctaca gaggtattct taattttttg aggaattaca ttcattgtga ttattgaaga
     4921 agaagacata ttcacactat attcaataat attctgcaac tctcttatat tccctggcca
     4981 cttatattcc atcatagcat caacaaccct gctatctatg tcaataacat ttttatctaa
     5041 tttttttgaa tactctttaa ccatatgctt gattataagt gggatatctt cttttctctc
     5101 tcttaaactt ggtaatttta taggtataac atttaatcta taatataaat cttctctaaa
     5161 agtgccattt tgaaccattt tttctaaatc tttatttgta gcagcaataa ttcttacatc
     5221 tattgagaca tttgatttac ttccaatttt attaagttcc ttttcttgta atactctcaa
     5281 taactttgac tgtaaatgca aactcatatc tcctatttca tctaaaaata tacttccctt
     5341 atgagctact tcaaacattc caagctttcc acctttttta gctccagtaa aagctccctc
     5401 ttcatatcca aataattcac tctctaataa attgtctggt atagcagcac agtttacagc
     5461 tataaaagga ttatctactc tattgctatg gttgtgtatg gctcttgcaa atagctcctt
     5521 acccgttcca ctttccccag taataagtac tgttgatgtt gatttagaag ctattagggc
     5581 ttccttttta gaatttctta tagctacact ttctccaatt atgttttcca attttattct
     5641 ataatcttta ttcatcttat tgtagttttt tatagcatct cttttatcta taaaatcaat
     5701 aacatatccc tttaactcat tttttaatac tattttattg atgttgtata ttcccttcga
     5761 atcatatccg tatcttttat aaaaaaaact acacgatttg tctttcttaa agttatttat
     5821 agaaggtttc tttataaaat ctaattcgac aaatatgttt ttgccagtta tactatcctt
     5881 tatattgaaa agctctttaa attttgaatt atatttatca atttttccat ccacatcaat
     5941 agacacaaca gctttgtcca tattgtttaa tagtgtctct aattttttct tttctaattc
     6001 aagctcataa gttttaattt gtgcttttaa tttattagat attaagtctg ccattttgcc
     6061 taaaaagttt ataagaccac ttttattatt ttctataatt tgtgcttgtt cttttgtaaa
     6121 ggctataagg cctataacgc catgagaatc gccatcacat ataataggac aacacacctc
     6181 tgcaaactct acacaagccc cacttttata gcattcccta catacatcac tactctttgg
     6241 gtcatcaatt agtattgtga tctgttctgt aagtgatttt ttaaaagccg tatatttatc
     6301 tactttctca cctattttgt ctatatatac tcctgtacct gcaatcctaa ataactcctt
     6361 atttactata gttacatcta tattgagtac tgatactata gcttcagcta tattttgaat
     6421 atcagatttt atatattcta aacctatcat aaccatcatt ccttattttt ctttaacata
     6481 agtatttgtc ttatttcttt aagaatcaag tatatcttat ttaaagtaaa ttggattgac
     6541 atcaggatta caaagagcat aacaattatt attaactcat aatttatttc catctaaaca
     6601 tctccttata acaaaagact aggcttaacc tagtctttta tattatatcc atatttatat
     6661 tttaactcat ttatcttctt tttcccacat aaatatattt taatttattt gactctttaa
     6721 tcataccatc aaagtttaat cttgaaaata tattaaatac ttcatgtgga tagtgaatct
     6781 ttgcaacaga accacaaaat ttatgtttaa gataatttaa cttaactttt aaattcttta
     6841 aatttaaatc ctctaatata acctctccat tgccctttaa cactctttgt atttcagatg
     6901 aagctttttc acaatctgta ataacattaa attggtcaac tattattatc ttgttgaact
     6961 gattatcatc aaatggtaga aactctaact tacctttaac tacattgcaa ttttcattat
     7021 acactagaga ataacagaca tcttcataat tactattctc cagtatagtt acagaagttc
     7081 caagacctct caatctcttg cctaggtttc ctatatgacc aacaagcaat attttatcac
     7141 ctacattata ctcaattgta tccaaaaaac attctaattc atcagatgta tatttagctt
     7201 taactggctg tttatttatc tgacataata tgcttttcac aatgcttcac ctcttcattt
     7261 cctcaattat aaattatgta cagattacat tataataaat ggcgagcaac tattcaagag
     7321 atatttcatt agtatttttt taaacaatct ttatacattt taatatgata ttataccaaa
     7381 tttattagga ttttattcat aaaaatcagt ctatttttta tgtttattca ttgttatttt
     7441 catccaattt ttttattttc gatatggata cttcatatgc catttttttg accacattat
     7501 tttcatctat ttttttctca tattctctac tttgaaccct accccaaagc tgtattctat
     7561 caccaacctt tagattcttt gcaaatttag catttctacc ccaaactatt gatggtatat
     7621 aatcagattt attataaggt ctgtttatag caactaataa atcggttatt tcccttccta
     7681 aaggtgtctt cctatacaca ggttccttac atacatatcc atctaaaaat atactattag
     7741 ggtctttatt ttcctcgtct atgcttttta tttctctgac aaacactgta agcactaatc
     7801 tatttttatt atttaatgtt ttgttatatg atcttaattg gcctacgaca ttgactaaat
     7861 tttgtttatc aaggtcaata tcttggagaa gtctttctga tacagtcatt ggaagtatat
     7921 caaaagaatc gcttagtctg tttattttta ttttcatatt ataaaatttt tctccaaata
     7981 tctcatggct aaattctagc ttattgtcta actcccctct taagtttact acgttattct
     8041 catctttaac agctatcata ttttatccct ccacaattgt taattcttat atttctggta
     8101 tcagcttata taataaatat atttataagg gatgtatttt attacaaatc tttgatt
//
LOCUS       DAMJWP010000161         8100 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.167, whole genome shotgun sequence.
ACCESSION   DAMJWP010000161 DAMJWP010000000
VERSION     DAMJWP010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8100
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.167"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..449
                     /locus_tag="PC700_002760"
     CDS             <1..449
                     /locus_tag="PC700_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2582055.1"
                     /translation="KQEPCDFSHGRFRFGGKIIRRNEKTKFDWLSRDKEQVEKYINDP
                     FCGVVCSCGFFYDLVQGLKEIEDKENLKKVPLDIPIYIISGDKDPIGKNGKGVLRLRD
                     RYIKLGVKDVTCKLYKDGRHELLNEINREEVFEDIICWLNNKIEIL"
     gene            914..2128
                     /locus_tag="PC700_002761"
     CDS             914..2128
                     /locus_tag="PC700_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2582056.1"
                     /translation="MQIKIGKVIQRLRKERNLTQEQLAKFIGVSTPAVSKWESGNSYP
                     DIELLPLLADFFNVSIDKLLNYKIDLSEEKVMKIYKELESGFARIEIDLSTEEPKEEF
                     RQDLESVKKLSNMYIEKYPKSYLLKLRICSLYQMYSYKFGKSELNDRVKETTNILEDI
                     VRNTDDIQIKETALIILSNAYCMLEDYEKAELYLNMIHKSIGDTSVNLAMIYLKQNRL
                     EEAEILLQNKLFSNIFNISMDCKGIINVYKNQYKELKKKLENRNFNKNAIEREMEYIK
                     NKLLWYANLSLEIKKMLSEDKGAFFSMYVDYMELSLIFLFFNMKEEAKKALYSLKEIL
                     EKYPIHESLDVSQMRFFDKVESKNLYTFNIYTNLLIVLNDDSYNELREEPIFKDVIEK
                     ILDMEKMLKNKE"
     gene            complement(2548..3069)
                     /locus_tag="PC700_002762"
     CDS             complement(2548..3069)
                     /locus_tag="PC700_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2582057.1"
                     /translation="MKFERLRTSENKLYYKAMELYKISFPFHEQRKSYLQTEILKNKE
                     YQFNLIYDKNQFVGIILCWETDNFIYVEHFCIFPEMRNNRYGQRALELLNKKGKTIIL
                     EIDLPIDEISIYRKAFYERVKYKTNNYEHIHPPYHEKFNGHNLIVMSYPEKLSKMEYD
                     KFNHYLRNNVMSF"
     gene            3425..4222
                     /locus_tag="PC700_002763"
     CDS             3425..4222
                     /locus_tag="PC700_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HDF2582058.1"
                     /translation="MEKREKRIIISSLLSVSILMGLVSIYSILNKEDIILTVKGQEQK
                     VSSFKKTVEELLDEQGVKYNSEDKINPSLDTELKDDMKIKVVKVTKSKKEEIEKIPFD
                     TKHVNDSNLLKGKSKVYQEGQEGEKKLVYNLTYHDGKLVKKVLSKEVISKEPTTKIIK
                     YGTKEKVLIASRGANIRGGKHMKVVATAYAGDTITSTGTTPRWGVIAVDPRVIPYGTK
                     VYIPKLGMTFVAEDCGGAIKGNRIDIFMNSEGKASNWGRKSIDIYLH"
     gene            4332..4922
                     /locus_tag="PC700_002764"
     CDS             4332..4922
                     /locus_tag="PC700_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDF2582059.1"
                     /translation="MKIVCLGDSLTYGFGVSRSNSWTNIVNEETRLEIVNKGINGDTT
                     SGMLARFNEDVVKNSPDIVFIMGGTNDFIAGAGNEIINSNIMAMVHQSYSKNIIPIIG
                     IPLKPDIPNVREDWSCFTDFNIVSQNLESYSYWIKKFCMTFNTNFADFYSEYNKNMEL
                     EGYKKLYFDGLHPTKEGHRIMADIFINSINKYIEEL"
     gene            4988..6685
                     /locus_tag="PC700_002765"
     CDS             4988..6685
                     /locus_tag="PC700_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDF2582060.1"
                     /translation="MDYTKTYEEWIKGSYFDEDTKLELENIKNNEKEIEDRFYKDLEF
                     GTAGLRGIIEAGTNRINKYTVRRATFGLANYILENTTKEETSRGVVIAHDNRHKSRQF
                     CIESANTLAACGIKAYIFDSLRTTPELSFAVRSLNAIAGIVITASHNPPEYNGYKVYW
                     EDGAQVMPEIANAITEKVNSIHDYSTIPTLTEANKNLVVLLDESQDTKFIEAVKSQII
                     RKDLVKNVGKSFKIVYTPLCGTGNVPIRRALKEVGFENIIVVPEEENPDPNFAGLDYP
                     NPEEKKALNRGILLAKEKGADLVIATDPDCDRVGVAVKTTTGEYALLTGNQIGGMLTH
                     YIIEGLKENNKLKENPTIIKTIVTSEFGADIAKANNVDVLNVLTGFKFIGEKIKLFEQ
                     NKNRSYVFGYEESYGYLVGTHARDKDGVVSSLLISEMAAFYYSKGISLYDGLIELYKK
                     YGFFKEQTISLTLKGIEGVEKIKEIISYFRENQIDYINSIKVVDKKDYKNGIDNLPKS
                     NVLKYFLEDESWVAIRPSGTEPKLKFYIAVKGASDIEADKKIQGLKKYIDDMVEKLK"
     gene            6899..7738
                     /locus_tag="PC700_002766"
     CDS             6899..7738
                     /locus_tag="PC700_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HDF2582061.1"
                     /translation="MFGIDKKLIEYIIAKSNYSELSSKSAEVSFFLMLSIFPFLIFTI
                     SSIAYIPILHLNKYIALFRNMMPEGAFAVLSSIIVSAIDNRNLKFLAVSFVLTMWTFS
                     RAVKALIKGMNRAYKVKETRSFFKILSISFLFTIMLLVLIFLSMIFLVYGEKIGYFIF
                     NLVGLDEIFIKIWDILRYTVGIITIIVIFTLLYKYTPNKKLTIKESAPGAIFATFAWF
                     LVSFLYSYYTNYYANYEVIYGSIAEIIVLMTWMYFSSWSIVIGYEVNSRLYFRKIRHE
                     MLK"
BASE COUNT         3230 a          778 c         1350 g         2742 t
ORIGIN      
        1 tgaagcagga accctgtgac tttagtcatg ggaggttcag atttggtggc aaaataataa
       61 gaagaaatga aaagactaag tttgactggt taagtagaga taaagaacaa gttgaaaaat
      121 atataaatga cccattttgt ggagtagtgt gtagttgtgg ctttttttat gatttagttc
      181 aagggttaaa agaaattgag gataaagaaa acttaaaaaa agtgccactt gatataccta
      241 tttacataat atctggagat aaagacccca taggaaaaaa tggtaaagga gtcttaagac
      301 taagggatag atatataaaa ttaggagtaa aagatgtaac ttgcaaactt tacaaagatg
      361 gaaggcatga attgttgaat gaaataaata gagaagaagt ttttgaggat ataatttgtt
      421 ggttaaacaa taaaatagaa atattgtaat aaaaaaatat aatgatatat ttgctttaaa
      481 tttatttata tataatattt gaaaagtgga ttatgtatat gcaaagtaga aaagagagag
      541 cctagaagtg attttttgct ataatgccat ttattgaata ggtgggtgtt attataaata
      601 aattattaac actcagtatt attatcattg tatatggaat ttattttatt taaaattcct
      661 tgaaagttta aatatagtaa gttgtagtaa agagaaaatc taaatattta aatgctattt
      721 aatgtgaatc tattaattag gttatactta aacttaatta atagatttta tatttcaagc
      781 caatgagata gggttttaga ttaaaatata accaatggtt aaattacaga cattttgtta
      841 actttccctc gatttactag taaatatttt ttttatatac ttaaattaca acagatgtac
      901 tagggaggta tatatgcaaa ttaaaatagg taaagtcata cagcgtctta gaaaagaacg
      961 aaatttaact caagaacaat tagcaaagtt tataggtgtg tcaacaccag cagtatctaa
     1021 gtgggagagt ggaaattctt acccagatat agaattattg cctttattag cagacttttt
     1081 taatgtgtct attgataagc ttttaaatta caagattgat ttaagtgaag aaaaagtaat
     1141 gaaaatttat aaagaattag aatcaggctt tgctagaatt gaaattgatt tatcaacaga
     1201 agaaccaaaa gaagaattta gacaagactt agaatctgta aaaaagcttt ctaatatgta
     1261 tatagagaag tatcctaaaa gctatttatt gaagctaagg atatgttcgc tgtatcagat
     1321 gtattcatat aaatttggta agagtgaact taatgacaga gtaaaagaaa caacgaatat
     1381 tcttgaagat atagtgagaa atacagatga tatccaaata aaagaaacag ctcttattat
     1441 attatcaaac gcatattgta tgttggaaga ttatgaaaag gcagagttgt atttaaatat
     1501 gatacataag tcaatagggg atacaagtgt taatttagct atgatatatt taaagcaaaa
     1561 tagacttgaa gaagcagaaa tattgcttca aaataaattg tttagtaata tatttaatat
     1621 aagcatggat tgtaagggaa taattaatgt atacaaaaat caatacaagg aattaaagaa
     1681 aaaattggag aatagaaatt ttaataaaaa tgcaattgag agagaaatgg agtatataaa
     1741 aaataagttg ctgtggtatg caaatctttc tttagaaata aaaaaaatgc tcagtgaaga
     1801 taaaggtgca ttttttagta tgtatgtgga ttatatggaa ttatctctaa tttttttatt
     1861 ttttaacatg aaagaagaag caaaaaaagc cttatatagt ctaaaagaaa tattagaaaa
     1921 atatcctata catgaaagtt tggatgtaag tcaaatgaga ttttttgata aggtagagtc
     1981 taaaaattta tatactttta atatatatac aaacttgtta attgtactta atgatgacag
     2041 ttataatgag cttagagaag aaccaatttt taaggatgta attgaaaaga ttttagatat
     2101 ggaaaaaatg ttgaaaaata aagaataagt aaaaatttaa tataggactt aaagattaat
     2161 caaattaaaa gaaagtgttt caaaataaaa atttgaatca ccttttttgt tggtcttaaa
     2221 ccgttattta gaaaaataga ggtttcaaaa tagaaatctg aattatctgt attattacac
     2281 aaaaatagaa acctaaattt ataaaaatag aataaagttt gttatataaa atgtaatttt
     2341 gtctataaaa tctcacatgt tttcttaact ttaagcgtga aaaagaggat gtatcatgaa
     2401 ttttttagtt catgatgcat ccttatttgt atgaaatcca atatatcttt attatttaaa
     2461 caatggaaaa gagtcttatt tctattttga gacatctctt ttatttaatt atgaagtttt
     2521 atacagttca ataaatagga attatattta gaaactcatg acattatttc ttagatagtg
     2581 attaaattta tcatattcca ttttagataa cttttctggg taagacatta caatcaggtt
     2641 atgcccattg aatttttcat ggtatggtgg atgaatgtgt tcataattat ttgtcttata
     2701 ttttactctt tcatagaacg ccttgcgata aattgaaatc tcatcaattg gaaggtcaat
     2761 ttctaaaata atagtttttc ctttcttatt taaaagctct aacgctcttt gtccatatct
     2821 attattgcgc atttctggaa aaatgcagaa atgttctaca tagataaagt tatctgtttc
     2881 ccaacataaa attatgccga caaactggtt tttatcataa attaaattga attgatattc
     2941 tttatttttc aaaatttctg tttgcaaata agattttctt tgttcatgaa atgggaaact
     3001 aattttatat aattccatag ccttgtaata taatttattt tcactggttc ttaaccgttc
     3061 aaatttcact ttactcctcc taatcacgaa aattccaatt tatttatgag ttcattttac
     3121 catagtcagt atttgcatac aatgtgtttt aagttgagtg tcatttcttt cttttattta
     3181 tgcttatatc tcatagacta agagataatt gcttaagaca taactaaaaa ctattatatt
     3241 aagctttgat aatctaaaaa cagtatattt atggactttt ctaggtctaa aagtaatctt
     3301 aataatttta gaagttacaa aaaagttaca taaatttgga aagctataat atacatatat
     3361 aatataaggt taaaactgta atgaataata ttaatttaaa atttgaacat ggaaggagat
     3421 tattatggag aaaagagaaa agaggataat aatatcatct ttattaagtg tatcaatttt
     3481 aatgggtttg gtaagtatat attctatatt aaataaagaa gacataattt taactgttaa
     3541 gggtcaagag caaaaagtat cttcctttaa gaaaacagtt gaggaacttt tggatgaaca
     3601 gggtgtaaag tataattctg aagataagat taatccaagt ttagatacag aactaaaaga
     3661 tgatatgaaa ataaaagttg ttaaggtaac taaaagtaaa aaagaagaga ttgaaaaaat
     3721 tccatttgat acaaagcatg taaatgatag taatttgtta aaaggaaaat ctaaagttta
     3781 tcaagaaggt caagaaggag aaaaaaaact agtctataat ttaacttacc atgatggaaa
     3841 attagtcaaa aaagtcttat caaaagaagt aatatctaag gagccaacta caaaaattat
     3901 aaaatatgga actaaagaaa aagtactaat agcatcaaga ggagcaaata taagaggagg
     3961 caaacatatg aaagtggttg ccactgcata tgcaggagat acaataacat ctactggtac
     4021 aactccaaga tggggtgtta ttgcagttga cccacgtgta ataccatatg gaacaaaagt
     4081 atatatacct aaactgggta tgacttttgt agcagaagat tgtggaggtg caattaaagg
     4141 taatagaata gacattttta tgaacagtga aggaaaagct tcaaattggg gaaggaaaag
     4201 tatagatata tatctacatt aaattagtga atagaataac tttacttgaa ggacaaaaaa
     4261 gtataaaata tgaattaact aaactgttag attattctta aaacataaaa atatatctaa
     4321 ggggggataa gatgaaaata gtctgcttag gagacagttt aacgtatgga tttggagtat
     4381 cacgaagtaa ttcatggaca aacattgtaa atgaagagac tcgattagaa atagtaaata
     4441 agggtataaa tggtgataca acaagtggta tgttagctag gtttaatgaa gatgttgtaa
     4501 aaaactctcc tgatatagtt tttataatgg gtggcactaa tgattttatt gctggtgcgg
     4561 gaaatgaaat tattaattct aatataatgg ctatggtgca tcagtcatat tcaaaaaata
     4621 ttatacctat aataggtata ccattaaaac cagatattcc aaatgttagg gaagattgga
     4681 gttgttttac tgattttaat atagtatctc aaaacttaga atcatatagt tattggataa
     4741 aaaaattttg tatgacattt aatactaatt tcgcagattt ttattcagaa tataataaaa
     4801 atatggaact agaaggatat aaaaaattat attttgatgg gttacatcca accaaagagg
     4861 gtcatagaat aatggcagac atttttatta attcaattaa taagtatata gaagagttat
     4921 agattacatt aaaatgcttg tttatgcgtc agttgcaata aatacataca gtagagaggg
     4981 gtttattatg gattatacta aaacttatga agagtggata aagggttcat attttgatga
     5041 agatacaaag ctagagttgg agaatataaa aaataatgaa aaagaaatag aggatagatt
     5101 ctataaggat ttagaatttg ggactgcggg tcttagagga ataattgaag caggaacgaa
     5161 tagaataaat aagtacacag ttagaagagc tacttttgga ttggcaaact atatattaga
     5221 aaacacaaca aaagaagaaa caagtagagg agtagtaata gctcatgata atagacacaa
     5281 gtctagacaa ttctgtatag aatctgcaaa tactttggca gcatgtggta taaaagcata
     5341 tatatttgat agtttgagaa ctactccaga attatctttt gctgttagaa gcttaaatgc
     5401 aatcgctggt atagtcataa cagcaagcca taatcctcca gaatataatg gatataaggt
     5461 atactgggaa gatggagcac aagttatgcc agagattgca aatgcaataa cagagaaagt
     5521 aaatagtata catgattata gtacaatacc aaccttaacg gaagctaata aaaatttggt
     5581 agttttgttg gacgaatcac aagatactaa atttatagaa gcagtaaaaa gccaaattat
     5641 aagaaaagat ttagttaaaa atgtaggtaa aagctttaag atagtttata cacctctttg
     5701 tggaacaggg aatgttccaa taagaagagc attgaaagaa gttggttttg aaaatataat
     5761 tgtagttcca gaagaagaaa atccagaccc aaattttgca gggcttgatt atccaaatcc
     5821 agaagagaaa aaagctttaa atagaggtat attactcgca aaagaaaaag gagcagacct
     5881 tgtaatagca actgacccag attgtgatag agtaggtgta gcagtaaaaa ctactacagg
     5941 tgagtatgct ttacttacag gaaatcaaat tggtggtatg ctgacacatt atataataga
     6001 aggattaaaa gaaaataaca aattaaaaga aaatcctaca ataataaaaa ccatagttac
     6061 atcagaattt ggagcagata tagctaaagc aaataatgtt gatgtattaa atgttcttac
     6121 aggttttaaa tttattggtg aaaaaataaa attatttgaa caaaataaaa atagaagtta
     6181 tgtatttgga tatgaagaaa gttatggtta cttagttggg actcatgcta gagataaaga
     6241 tggtgtagtt tcttcattgt taatatctga aatggcagca ttttactatt caaaaggtat
     6301 aagtttatat gatggtttaa tagaacttta taaaaagtat ggatttttca aagaacagac
     6361 tatatctttg actttaaaag gtatagaagg tgtagaaaag ataaaagaaa taatatctta
     6421 ttttagagaa aatcaaattg attatataaa tagtattaaa gtagttgata aaaaagatta
     6481 taaaaatggt atagataatc ttcctaaatc taatgtttta aaatactttt tggaagatga
     6541 atcttgggta gctataagac catctggaac agaaccaaaa cttaaatttt acatagctgt
     6601 aaaaggagct agtgatattg aagcagataa aaagattcaa ggtttgaaaa agtatataga
     6661 tgacatggtc gaaaaattaa aataaaatat tattatttta taagataatt ttgtaataat
     6721 atttatttag ctgaaaatta taatttactt tcattgagta gaatatggaa atgttagtag
     6781 aattgttatt ttcagctttt atttgtgtta tttttactta aataattata ttatagaatg
     6841 aatctgagta taattaatat tgtataatga aattaatttg atagaaaagg ggatatttat
     6901 gtttggtata gataagaaat tgattgaata tataattgca aaatccaatt acagtgaatt
     6961 aagttcaaag tcagcagaag tatctttctt cttaatgttg tccatatttc cttttttaat
     7021 atttacaata agttctatag cttatatacc gattcttcat ttaaataagt atatagcttt
     7081 gtttagaaat atgatgccag aaggtgcttt tgctgtgtta tcttcaatca tagtttctgc
     7141 aatagacaat agaaacctga aatttttagc agtcagtttt gtacttacta tgtggacatt
     7201 ttcaagagca gtaaaagctc ttattaaagg aatgaataga gcctataaag ttaaagaaac
     7261 taggtctttt tttaaaattt tatctatatc atttttattt acaataatgc ttttggtcct
     7321 tattttttta tctatgatat ttttagttta cggggaaaaa ataggttatt ttatttttaa
     7381 tcttgtagga ttggatgaga tatttataaa aatatgggat attctaagat acactgtagg
     7441 tataataaca ataatagtga tatttacact tttatataaa tacactccaa ataaaaagtt
     7501 aactattaaa gaatctgcgc caggtgctat ttttgctact tttgcttggt ttttagtatc
     7561 atttttatat tcttattata caaattacta tgctaactat gaagtaatct atggaagtat
     7621 agctgaaata attgtactta tgacctggat gtattttagt agttggtcta tcgttattgg
     7681 ctatgaggta aattcaagat tgtattttag aaagataaga catgaaatgt taaagtaaag
     7741 tatttaaaat aaaagagagt atttagtatt ataaatgtaa ttgaattaat aaagaaatat
     7801 tgacatttaa gttaaaaagt ctagaatttt tctgggcttt ttttattttt agtataactt
     7861 gacaaatttt atttaatggg atagttttaa tttataatca tctttgaaaa gatgactatt
     7921 gataagtaag gtataaaatt ggtaagtatc aaaaaatata ctaaataaaa attatagtcc
     7981 taatgagtta atttctgtat tttcaaataa tgcagtattt agtcatgtgt ttttatatgc
     8041 gcctgtattt ccaattacca atatattaga ataagctact gtaattagat ttagaaaaaa
//
LOCUS       DAMJWP010000162         7940 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.168, whole genome shotgun sequence.
ACCESSION   DAMJWP010000162 DAMJWP010000000
VERSION     DAMJWP010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7940
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.168"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            9..344
                     /locus_tag="PC700_002767"
     CDS             9..344
                     /locus_tag="PC700_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2582062.1"
                     /translation="MYNIDDMIYNCPVEALSNILGKKWVAIIIWKIQEDKKRFGELQR
                     AIPDCSKKMLVQQLDFLIEQGIIVNQKKLVNNVVESTYFLSESGLRLLPVMEKMIMWS
                     NKNLNCDKY"
     gene            481..1758
                     /locus_tag="PC700_002768"
     CDS             481..1758
                     /locus_tag="PC700_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HDF2582063.1"
                     /translation="MQNVNLFLVFIEGIVSFFSPCILPILPIYLSILSNSSVENLKEG
                     KTSFIGSSLFKNTIFFALGISTTFFILGSSVKVLSMFFNENKDLIMFIGGIIIIIMGL
                     FYMGIIKSSILNREKRFNVKFKEMKAITAFILGFTFSFGWTPCIGPILASVLVMVSSS
                     SNHLSANLLIAVYTIGFILPFIITAMFYSKLFKTIDKIKSNMEIIKKIGGIILIVSGI
                     LMMVNGFGSISKHFNTSQNSKIESKQEENKRENSTDKEENSDGNNSQKDSNNDNNDNG
                     SNDEDRIKSIDFTLTDQYGKTHKLSDYEGKVVFLNFWATWCPPCKEEMPYIEQLYKDY
                     NKNNDDVVILGVASPNLGREGSREHVVNFLKDQGYTFPVVLDEDGALAYQYGINAFPT
                     TFIIDKEGYVTQYIPGAMDKATMASFIENQRNK"
     gene            1879..3267
                     /locus_tag="PC700_002769"
     CDS             1879..3267
                     /locus_tag="PC700_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF2582064.1"
                     /translation="MKKTFDAIIIGFGKGGKTLAGDLANRGLKVALIEKSNKMYGGTC
                     VNVACIPTKSLENSANSVKTKNINSWDEVQAEYEKAIDKKETLITKLREANYNKLNSN
                     ENVTIFTGMGTFIDEKTVQVKTENEIYELVADNIFINTGSRPFIPNIKGIENKNIVYD
                     SESLMNLRVLPKKMTIIGAGFIGLEFAGIYSSFGAEVTILNSNNGILPNEDVEDSEEI
                     IKLLAKRNVKIVNNANIKEIKEVSESAIVEYEVDGKSKELTSNMILVATGRKANTEGL
                     GLENAGIELNERGFIKVSETLKTNKEHIWAIGDINGGPQFTYISLDDYRIVINQLFGD
                     KTRTTNDRKNIPNSIFISPAFSRVGLNVKQAKEKGYEVLVAKMPVEAIPRAKQIGKAD
                     GFIKIVIDKKSNKILGASMICENSSEIIHLIQLAVDLEVEYTYLRDRVYAHPTMTEAL
                     NDILSPNMIKEV"
     gene            3638..4351
                     /locus_tag="PC700_002770"
     CDS             3638..4351
                     /locus_tag="PC700_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017208807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HDF2582065.1"
                     /translation="MNKISKRGFDDSDKRWFSSKELTRLIKAKEEIEWLLNRDYKIDP
                     VVTFVGDRYQFSIRQRDALKRAVCTDEKNIIRQSKRLFLDKIKEDTVNIDGFNLIISI
                     EVALSGGTLVIGSDGNIRDLAGLRGTYKIIDKTEEALNLIGKFFNKYNAQNLKFYLDA
                     PVSNSGNLKYRILEHAKTWGIETEVELVKNADVVLEKLDRVVSSDAVIVDKCISYFNV
                     ARGIIEEYIKECNIINLNK"
     gene            4469..5338
                     /locus_tag="PC700_002771"
     CDS             4469..5338
                     /locus_tag="PC700_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDF2582066.1"
                     /translation="MRGWICVKLTKLNIKKYRAPIYKYALANVNLRSAKSTNSSIITV
                     IPQGAKMEVLDEEDDWIKVMYNSQEGYVYKDLVSVSEYAWSNLNLREDKSITSNIITV
                     IPEKSRVEVLQVDGDWSKIVYDDKIGYVFNYFLSTDGNKPNELDYKYFYTDMIKFVNE
                     NNIKSTSDYLIVTDLRNKYTYIFKKDNGGWGQLYKWQCTIGKPETPTITGIFYISGRK
                     PSFGTDEYSVKYATRIKGGYYYHSVLYDSTGSYIIDGRLGEALSHGCIRLSTENAKWI
                     YDNIPDTTTVIIH"
     gene            complement(5493..6638)
                     /locus_tag="PC700_002772"
     CDS             complement(5493..6638)
                     /locus_tag="PC700_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDF2582067.1"
                     /translation="MSTYYVPPINLLGKGCLNEAKDSIKSLGTKKAFVVSDKFLVSNG
                     TVKKVTDILSQIDVDFVVYDEVKQNPTVTNVDKGLKILKSENCDFVITIGGGSPQDCG
                     KAISILATNGGDIRDYEGINKTSKKCLPIVAITTTAGTSAEVTINYVITDEDRHVKMI
                     MVDTNSLATMTVNDPELMISKPSNLTAATGMDALTHAVEAVVANGAYDVTDSTALYAI
                     KQIFKYLPRAVKNGNDIEAREQMCYACFLNGIAFSNAGLGNVHAMAHQLGGLYDLPHG
                     VCNAMLLPIVEEENAKSAPAKFRPIAEVIGMDVNNKSDKECVDFVIDKIKALSEEVGI
                     PKSLKDVGVDNPNFELLAENSMKDACAGANPVFFDKEKIIELFMKIS"
     gene            complement(6971..7747)
                     /locus_tag="PC700_002773"
     CDS             complement(6971..7747)
                     /locus_tag="PC700_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDF2582068.1"
                     /translation="MTSDNHRPTARILDILEALAESENGYTLTEIADVINAPKSSIFP
                     IVHTLNARKYITIDKNTSKYSIGIRSYTLGSSFFEKRTIFNYIIDEMTNIVNSCSETC
                     QLGILDNNEVLYIGKVDSPEPIRLISYVGKKIPANCTGLGKALLCDFDKEKLISLYPD
                     GLKGFTEKSITDFDVLYDQLLEVQRTQIASECEESMEHLKCLAVPIRKNGCIRAAVSV
                     TLPLFRANDEKIELIKKLLLNAQKNIQSMMEERNEDIVIN"
BASE COUNT         3057 a          917 c         1296 g         2670 t
ORIGIN      
        1 agaataaaat gtacaacata gatgatatga tatataattg tccagtagaa gctttatcaa
       61 atattttagg taaaaaatgg gtggctataa ttatatggaa aatacaagaa gataagaaac
      121 gatttggaga gctacaaaga gctataccag actgtagtaa gaaaatgtta gttcaacaac
      181 tagatttttt aattgaacag gggattatag tcaaccaaaa aaagttggtt aataatgttg
      241 tagagtcaac atatttttta agtgaatcag gattacgatt gcttcctgtc atggaaaaaa
      301 tgattatgtg gagcaataaa aatttaaatt gtgataaata ctaaaattta ctctgtaagt
      361 aatgaaaatt taattaagct atgagtagaa aaatgtatta aaattacata attttttagt
      421 tagattttaa tttagaaatg ggattgtaat attggaaata aaataaggag gaattttaat
      481 ttgcaaaatg taaatttatt tttagtattt atagaaggta tagtatcatt cttttcacca
      541 tgtatactgc caattttgcc tatttatttg agtatattgt caaatagtag tgtagaaaat
      601 ctaaaagaag gaaagacgag ttttatagga agttctttat ttaaaaatac tatatttttt
      661 gctcttggaa tctcaacaac attctttata ttgggttcat cagtaaaagt attgagtatg
      721 ttttttaatg aaaataaaga tttaataatg tttattggtg ggattataat tattatcatg
      781 ggattatttt acatgggaat aattaaatct tcaatattga atagagaaaa aagatttaat
      841 gttaagttta aagaaatgaa agcaataaca gcttttatat taggttttac gtttagtttt
      901 ggatggacac cttgtattgg tccaatattg gcatctgtat tagtcatggt ttcaagttca
      961 agtaatcatc ttagtgccaa tcttttaata gcagtatata ctattggttt tattttgcca
     1021 tttataatta cagctatgtt ttacagtaaa ctatttaaaa ctatagataa gataaaatct
     1081 aacatggaaa ttattaaaaa aataggtggg attatactta tagtatcagg aatattaatg
     1141 atggtaaatg gatttggaag tataagcaaa cactttaata cgtcacaaaa tagcaaaatt
     1201 gaaagcaagc aagaagaaaa taagcgagaa aattctactg ataaagaaga aaattctgat
     1261 ggtaataata gtcaaaaaga ctctaataat gacaataatg ataacgggtc taatgatgaa
     1321 gatagaataa agtctattga ttttacttta acagaccagt atggtaaaac acataaatta
     1381 agtgattatg aaggcaaagt tgtattctta aatttctggg ctacatggtg tcctccatgt
     1441 aaagaagaaa tgccttatat agaacaactg tataaagact ataataaaaa caatgatgat
     1501 gtagtaattt taggagtagc ttcacctaat ttaggtagag aaggttcaag ggaacatgta
     1561 gttaacttct taaaagacca aggttataca ttccctgttg tacttgatga agatggagca
     1621 ttagcatatc aatatggaat taatgctttt ccaactacat ttataattga caaggaagga
     1681 tatgtaactc agtatattcc aggagctatg gataaggcaa ctatggcatc atttattgaa
     1741 aatcagagaa ataaataaaa gtatttaagt atatgtttat gaatcaattt aaggttacaa
     1801 aaaagtgcct aattgtacca cctaaataat cttgttatag taaaagtata aacaatacaa
     1861 gaaagggttg aattttatat gaaaaaaaca tttgatgcta taataatagg atttggaaaa
     1921 ggtggaaaaa cattagcagg agatttggca aacaggggat taaaagtagc tttaatagaa
     1981 aagtcaaaca agatgtatgg tgggacatgt gtaaatgtgg catgtatacc aacaaaaagc
     2041 ttagaaaatt cagcaaacag cgtaaaaaca aagaatataa attcttggga tgaagtacaa
     2101 gctgaatatg agaaagctat agataaaaaa gaaactttaa ttacaaaact cagagaagct
     2161 aactataata aattaaattc aaatgagaat gtaactatat tcacaggaat gggaactttc
     2221 attgatgaaa aaacagttca agttaaaact gaaaatgaaa tttatgagtt agtagcagat
     2281 aatatattta ttaatactgg ttcaagacca tttataccta atattaaggg aattgaaaat
     2341 aagaatatag tatatgatag tgagtctctt atgaatctaa gagttttacc taaaaaaatg
     2401 acaattatag gagctggatt tataggttta gagtttgctg gtatttatag ttcatttgga
     2461 gcagaagtta caatattaaa ctctaataat ggcattttac caaatgaaga tgttgaggat
     2521 tcagaagaaa taataaaatt acttgcaaag agaaatgtaa aaattgttaa taatgcaaat
     2581 ataaaagaga ttaaagaagt ttctgaatca gcaatagtag aatatgaagt agatggtaaa
     2641 tctaaagaat taacaagtaa tatgatactt gtagctacag gaagaaaagc aaacactgaa
     2701 gggctagggc tagaaaatgc aggtatagaa ttaaatgaaa gaggatttat taaagtctca
     2761 gaaactctta agactaataa agagcatata tgggctatag gagatataaa tggaggtcca
     2821 caatttactt atatatcatt ggatgattat cgcatagtta ttaatcagtt atttggagat
     2881 aagactagaa caactaatga tagaaagaat atcccaaatt ctatatttat aagtccagca
     2941 ttttcaagag ttggtcttaa tgttaaacaa gctaaagaaa agggatatga agtattagtg
     3001 gcaaagatgc cagtagaagc aattccaaga gctaaacaga ttggcaaggc agatgggttt
     3061 ataaaaatag tgatagataa aaaatccaat aagatacttg gagctagtat gatatgtgaa
     3121 aattcaagtg agattatcca tcttattcaa ttagctgtag atttagaagt agagtataca
     3181 tatttaagag accgtgttta tgcacatcca actatgacag aagctcttaa tgatattcta
     3241 tctcctaata tgataaaaga agtataacat aagtttaata tataaaccta aaattatcat
     3301 tataagtaga ttttagaaag aggctatctc aaaaaataga gataagtctc ttttttactt
     3361 tttaaatgat aaaaatatat ttgattttat aaaagaagat tatcttatga actaaaaagt
     3421 ttattttaaa acactaccta tttatgttat aaacttataa atataatata gaatctatta
     3481 ttctgtatta ttaagtgagg ttaagcgtta taattaatta taaatattga ataatctaaa
     3541 tggataagat aatggtatta aaaattatct taaaaggtga aataatctgt atttaatgta
     3601 gtacagttat atctttaaag caaggtggtg aaatgatata aataagatat ctaaaagagg
     3661 gtttgatgat agtgataaaa gatggttttc aagtaaagaa ttaacaagac ttataaaggc
     3721 taaagaagaa atagaatggt tattaaatag ggattacaaa atagacccag tcgttacttt
     3781 tgttggtgat agatatcagt tttctataag acaaagagat gcattaaaaa gagcagtctg
     3841 tactgatgaa aaaaatatta taagacaatc aaaaaggctt tttctagata aaatcaaaga
     3901 agataccgta aatatagatg gctttaattt gataatatcc atagaggtag cattatctgg
     3961 tggtactcta gtaataggaa gtgatggaaa tataagagat ttagcaggtc ttaggggaac
     4021 ctataagatt atagataaaa cagaagaagc tttgaatctg ataggtaaat tttttaataa
     4081 atacaatgca cagaatctta aattttattt agatgctcca gtttctaatt caggaaattt
     4141 aaagtatagg atattagagc atgccaaaac ttggggaatt gaaactgaag tagaattagt
     4201 aaaaaatgca gatgttgtat tggaaaaatt agatagagta gttagtagtg atgcagtcat
     4261 agttgacaag tgtataagtt attttaatgt ggctagaggt ataattgaag agtatattaa
     4321 agaatgtaat atcataaatt taaataaata gataaataga ggtgagaata tataagtata
     4381 tgtagcaata ttattagtaa tacaagtaaa gtttgaaaat aggagaacga atacatatcg
     4441 aatttcataa aatgttgtaa aaagacatat gagaggatgg atatgtgtga aattaactaa
     4501 actaaatatt aaaaaatata gagcacctat atataaatat gctttagcaa atgtaaattt
     4561 gaggtctgct aaatctacta attcaagtat cataacagta atacctcagg gagctaaaat
     4621 ggaagtattg gatgaagaag atgactggat taaggtgatg tacaattctc aagaaggata
     4681 tgtgtataag gatttggtat cagtaagcga atatgcttgg agtaacttaa acttaagaga
     4741 agataaatct ataacttcca atataattac agtaattcct gaaaaatcta gggttgaagt
     4801 ccttcaagta gatggagatt ggagtaagat tgtatatgat gataaaatag gatatgtttt
     4861 taattatttt ttatcaactg atgggaataa accaaatgaa ttagattata agtattttta
     4921 tactgacatg attaaatttg ttaacgaaaa taatattaaa agtactagcg attatctaat
     4981 tgtaacagac cttagaaata aatatactta tatttttaag aaagataatg gaggctgggg
     5041 gcaactttac aagtggcaat gtactatagg caagcctgag actccaacta taacaggtat
     5101 attttatata agtggaagaa aaccttcttt tggaacggat gagtattcag ttaagtatgc
     5161 tactagaatt aaaggtggat attactatca ttctgtgcta tatgattcaa caggaagtta
     5221 tataatagat ggcagacttg gtgaagctct tagtcatgga tgtataagat taagtacgga
     5281 aaatgcaaag tggatatatg ataatatacc agatacaaca acagttataa ttcattaaga
     5341 aaaattttga gtttttattg ggataatgat tctcaaatta ataggattat atattaactt
     5401 cttcataaag agtactattt aaaaagtgta ataagagagt gctattaaca aaacaaagat
     5461 attttgaggc actctcttat aaatatacat aattaagata ttttcataaa caattctata
     5521 atcttctcct tatcaaagaa aacaggatta gcaccagcac aagcatcttt catagagttt
     5581 tctgcaagca attcaaaatt aggattatca acacctacat cttttaaaga ttttgggatt
     5641 ccaacctctt cagaaagagc ttttatctta tctattacaa agtcaacaca ctctttatct
     5701 gatttattat ttacatccat tccaataact tcagctattg gtcggaattt ggcaggggct
     5761 gatttagcat tttcctcctc aactattgga agtagcatgg cattacatac tccatgaggt
     5821 aaatcatata aacctcctaa ttgatgagcc atagcatgta catttccaag acctgcatta
     5881 ctaaaggcaa ttccatttaa aaaacaagca taacacattt gttctctagc ttctatgtca
     5941 ttaccatttt taacagctct tggaaggtat ttaaatatct gctttatagc atataaggca
     6001 gttgaatctg taacatcata agcaccatta gctactacag cttctacagc atgagttagt
     6061 gcatccatac cagttgctgc tgttaaatta gatggtttgc taatcattaa ttctgggtca
     6121 tttactgtca ttgtagctag tgagttggta tcaaccatta tcatctttac atgtctgtct
     6181 tcatcagtta taacatagtt tattgtaact tcagcagatg taccagcagt agtagttata
     6241 gctacgatag gaagacattt tttagaagtt ttgttgatac cttcatagtc tctaatatct
     6301 cctccattag tagctaatat agagatagct ttaccacagt cttgtggaga acctcctcct
     6361 atagttataa cgaagtcaca attttctgat tttaatattt taagtccttt atccacatta
     6421 gttacggttg gattttgttt aacttcatca taaacaacaa agtctacatc tatctgagat
     6481 aaaatatctg ttactttttt tacagtacca tttgaaacta gaaatttatc actaacaaca
     6541 aatgcttttt tagttcctaa tgatttaatt gaatctttag cttcattaag acatcctttc
     6601 ccaagtaaat ttattggtgg tacataataa gtgctcataa ctaaaatctc cttttatttt
     6661 cattgatttt tatgtaaaaa tatatatcta acaattttca ctaatttact cataatatat
     6721 cgtatcttag attgagatag ggttactggt tcttatataa gaacttaatt cgtaattacg
     6781 attatgatta gaatttaaca tagtattttt atcatgtcaa taaaaatata aaaaatatta
     6841 taaaaaaaac aaagttactg tagttaaatc agcaactttg tttttatagt ataaatcaaa
     6901 taaatacatt agtttaaaac aaaattttta attattatgt tttgatatct tccatataca
     6961 gaatattaat ctaatttatt acaatgtctt cattacgttc ttccatcatt gattgaatat
     7021 ttttctgagc atttaagagt aattttttta tcagttctat tttttcgtca ttagctctaa
     7081 ataaaggcag tgttacacta acagcagctc ttatacatcc attttttctt attggtacgg
     7141 caagacattt taggtgttcc attgattctt cacactctga agctatttgt gttctttgaa
     7201 cttcaagaag ctggtcgtat aaaacatcaa aatctgtaat tgatttttct gtaaaacctt
     7261 ttagaccatc tggatataag cttattagtt tttccttgtc aaaatcacat aaaagagctt
     7321 ttccaagacc tgtacaatta gcaggtattt tttttccaac ataagaaata agtctaattg
     7381 gttctggaga atctactttt ccaatataaa gaacttcatt attgtcaagt ataccaagct
     7441 gacaagtttc agaacaggaa ttaactatat tagtcatttc gtcaattata tagttaaaaa
     7501 tagttctttt ttcaaaaaat gaagacccta atgtgtaaga acgtatccct atagaatatt
     7561 tggatgtgtt tttatcaata gtaatgtatt ttctagcatt aagtgtatga actataggaa
     7621 agatgctact ttttggagca ttgataacat ctgctatttc tgtaagagta tatccatttt
     7681 cggattctgc taatgcttct aaaatatcta atatcctagc tgttgggcga tgattatctg
     7741 aagtcataaa tttccccctc tttttattaa tttaaagtat tagaatatgt tgattttagc
     7801 acactatacg atattttaca atgtagaaaa taaatatgta aatttatata taaataagct
     7861 cttgatttag atacagatag atattttttt agactgtaca ttaaggaata actaatttcc
     7921 aaaatatacc taaagaaagt
//
LOCUS       DAMJWP010000163         7938 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.169, whole genome shotgun sequence.
ACCESSION   DAMJWP010000163 DAMJWP010000000
VERSION     DAMJWP010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7938
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.169"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            27..101
                     /locus_tag="PC700_002774"
     tRNA            27..101
                     /locus_tag="PC700_002774"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Thr,seq:tgt)
     gene            116..200
                     /locus_tag="PC700_002775"
     tRNA            116..200
                     /locus_tag="PC700_002775"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Tyr,seq:gta)
     gene            210..293
                     /locus_tag="PC700_002776"
     tRNA            210..293
                     /locus_tag="PC700_002776"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:244..246,aa:Leu,seq:tag)
     gene            317..391
                     /locus_tag="PC700_002777"
     tRNA            317..391
                     /locus_tag="PC700_002777"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:349..351,aa:Gly,seq:gcc)
     gene            416..492
                     /locus_tag="PC700_002778"
     tRNA            416..492
                     /locus_tag="PC700_002778"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:450..452,aa:Arg,seq:tct)
     gene            502..577
                     /locus_tag="PC700_002779"
     tRNA            502..577
                     /locus_tag="PC700_002779"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:535..537,aa:Gln,seq:ttg)
     gene            586..661
                     /locus_tag="PC700_002780"
     tRNA            586..661
                     /locus_tag="PC700_002780"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:619..621,aa:Lys,seq:ttt)
     gene            664..752
                     /locus_tag="PC700_002781"
     tRNA            664..752
                     /locus_tag="PC700_002781"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:698..700,aa:Ser,seq:tga)
     gene            756..831
                     /locus_tag="PC700_002782"
     tRNA            756..831
                     /locus_tag="PC700_002782"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:789..791,aa:Phe,seq:gaa)
     gene            838..911
                     /locus_tag="PC700_002783"
     tRNA            838..911
                     /locus_tag="PC700_002783"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Met,seq:cat)
     gene            926..1002
                     /locus_tag="PC700_002784"
     tRNA            926..1002
                     /locus_tag="PC700_002784"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:960..962,aa:Ile,seq:cat)
     gene            1020..1096
                     /locus_tag="PC700_002785"
     tRNA            1020..1096
                     /locus_tag="PC700_002785"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1054..1056,aa:His,seq:gtg)
     gene            1105..1180
                     /locus_tag="PC700_002786"
     tRNA            1105..1180
                     /locus_tag="PC700_002786"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1138..1140,aa:Lys,seq:ttt)
     gene            1188..1261
                     /locus_tag="PC700_002787"
     tRNA            1188..1261
                     /locus_tag="PC700_002787"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1220..1222,aa:Cys,seq:gca)
     gene            1269..1345
                     /locus_tag="PC700_002788"
     tRNA            1269..1345
                     /locus_tag="PC700_002788"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1303..1305,aa:Arg,seq:acg)
     gene            1358..1433
                     /locus_tag="PC700_002789"
     tRNA            1358..1433
                     /locus_tag="PC700_002789"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1391..1393,aa:Val,seq:tac)
     gene            1509..1922
                     /locus_tag="PC700_002790"
     CDS             1509..1922
                     /locus_tag="PC700_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582069.1"
                     /translation="MYIVIGNEIVDSEELKIIIDENSKFKVEKDLSKSTKREDVIAYQ
                     LSIDLNYLDSLINEQCNLSSLSDEEKFDEYMTLSDELALDLEELMPKYTIINARAYKL
                     DEVDGIVKIILAVAYADLGHLKLSDVVKRLSRQVD"
     gene            2231..5662
                     /locus_tag="PC700_002791"
     CDS             2231..5662
                     /locus_tag="PC700_002791"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HDF2582070.1"
                     /translation="MLKKFNKILVANRGEIAIRIFRACSELGIKSVGIYSKEDKYGLF
                     RTKADESYLIGEGKGPIDAYLDMDGIIDLAKRKKVDAIHPGYGFLAENAEFARKCEEN
                     GITFIGPSSKVMNMMGDKINSKKIAKEVNVQTIPGVEKAIRSTEEAKEVANKIGYPVM
                     IKASNGGGGRGMRIVHREEDLELEYETACSESRKAFGEDIIFIEKYIADPKHIEVQIL
                     GDNYGNIVHLYERDCSVQRRHQKIIEYAPAFSLDDKVRKEICEDAVKLSKHVGYSNAG
                     TLEFLVDANGGHYFIEMNTRVQVEHTVTEMVTGIDIVQSQILIAQGYSLDSEEINIKS
                     QDDVEIRGYSIQCRITTEDPKNKFMPDTGKIQVYRTGSGFGIRLDGGNGFTGANISPH
                     YDSLLVKTISWDRTFQGAINKTIRSIKELRVRGVKTNVGFLVNVLNNPIFSNGKCSTK
                     FIDENPDLFEITESKDRGTKLLQFIGDVIVNDNACKEKPLFDALHDPRMDKDGSKSEG
                     SKILFDKLGKSAYIEKIKNDKKLLLTDTTMRDAHQSLLATRIRTYDLLKAAKPTEKYQ
                     KDLFSLEMWGGATYDVAYRFLKESPWRRLQKLREEIPSIMFQMLLRASNGVGYKNYPD
                     NVIEEFTKESARQGIDVFRIFDSLNWVENMKPSINTALETGKIVEATMCYTGDILDKT
                     KTKYNLEYYIKMAQELESLGADIIAIKDMSGLLKPYSAYTLVKELKKNVKAPIHLHTH
                     DTSGNGVATCLMASEAGVDIIDAALESMAGLTSQPSLNAIVEALKNTERDTGIDLFGY
                     DELGKYYKDLRKVYNKFESDLTNSCAEIYNFEIPGGQYTNLKPQADSLGLVNRFDEVK
                     EKYKEANEVVGDIIKVTPSSKVVGDLAIFMTKNKLDKDNIIEEGKNLSFPDSVVDYCK
                     GMIGQPEGGIPKDLQEVVLKGEEAITVRPGSLLPAEDFDEIAKYLNEKYDINANIRNV
                     ISYALYPKVYEDYIKHLQHYNDISKLESDVFFYGLNKNEECEVEIEEGKVLTIRLVEI
                     GEVKENGFRTIGFELNGMVREVEIKDKNFSGKINNVEKADMNDPLQIGASIPGKVIKI
                     MVKEEDEVKANQPLIVIEAMKMETIIVAKTDGVIKSIKVKEDDMVEDKQLLMIMK"
     gene            5904..7832
                     /locus_tag="PC700_002792"
     CDS             5904..7832
                     /locus_tag="PC700_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDF2582071.1"
                     /translation="MKVYDSKMLRNVAVLGHSGCGKTNLIETIAYTANTNKIPKLTDK
                     VNMTYSMGLIPIEYNDYKFNLLDTPGYFDFSGDVVSSLRASDAAIIVIDATAPIQVGT
                     EKSLELTESIPKIMFINKIDNEKARYKDAIAMLREKYNNKIVPMISPIYKDKNFVKLH
                     NVFENIDDLEGEFKEQAMSVKEALMELIAETDDQILDKYFNGEELTTEEIQKGIIIGI
                     QRGDIIPVICGSTINNIGTKEILDTISSYLEPIFTEESKPFRGLVFKTMVDPFVGKMS
                     YIKITEGVLSKDKDVFNINKNVKEKIANIYTLRNSELVEIEKAKAGDIVVITKVNSLK
                     TGDTISADKDAEALEKIDFPKPQIYYAVTPKNKGDEEKVASVLNKLVEEDPTLHWYRN
                     TETKQALLGGQGELHIKTIKNKMKDKFGVDVELNDLKVPYRETIKGTADVQGKHKKQS
                     GGHGQYGDVKIRFERCESDFEFTEEIFGGSVPKQYIPAVEKGLKDSMQKGILAGYPVT
                     NIKATLYDGSYHDVDSSEMAFKMAASAAFKKGMEEAHPILLEPIMKLKITVPEEYMGD
                     VMGDINKRRGKIFGMEPDDKGKQIIFAEAPQAETFKYAIDLRAMTQGRGYFEMELERY
                     GEVPSQFAEKIIGLATAK"
BASE COUNT         3102 a         1017 c         1596 g         2223 t
ORIGIN      
        1 ccccttccag gtcgccaaaa taaaatgctg gtgtggctca acggtagagc agctgacttg
       61 taatcagcag gttgtaggtt cgattcctat caccagctcc atgtaaaatt aatacggagg
      121 atttcccgag cggccaaagg gggcagactg taaatctgtt gtcatcgact tcggtggttc
      181 gaatccacca tcctccacca aaaaaatgcg cggatgtggc ggaattggca gacgcactag
      241 acttaggatc tagcgccatt ggcgtggggg ttcgactccc ttcatccgca ccatttttat
      301 ttaatttaat aaacgagcgg gaatagttca gtggtagagc gcaaccttgc caaggttgaa
      361 gtcgcgagtt cgaatctcgt ttcccgctcc aaattttatg tttatgttgt aatgtgggtg
      421 catagctcag ctggatagag caacgccctt ctaaggcgtg tgtccggggt tcgaatccct
      481 gtgcgctcac cacatatata ttggggatta gccaagtcgg taaggcacat gactttgact
      541 catgtatgcg taggttcgag tcctgcatcc ccagccaaac agtatggtct attagctcag
      601 tcggtagagc acctgacttt taatcagggt gtcccgcgtt cgagtcgcgg atagatcacc
      661 aatggagagg tgtccgagtg gtttaaggag ctggtcttga aaaccagtga cttcgaaagg
      721 ggccgtgggt tcgaatccca ccctctccgc caaatgccca gatagctcag tcggtagagc
      781 aggggactga aaatccccgt gtcggtggtt cgattccgcc tctgggcacc aaaatgtggc
      841 ggtatagctt agttggctag agcgttcggt tcatacccga aaggtcatag gttcgactcc
      901 tattaccgct actaaaattt aatgtgggcc attagctcag ttggttagag cgcccggctc
      961 ataaccggta ggtctggggt tcgagtccct gatggcccac cacattaaat taacttatgg
     1021 tgggtatagc tcagttggtt agagcgccag attgtggctc tggaggtcgt gagttcgact
     1081 ctcattatcc accccaactt atatggtcta ttagctcagt cggtagagca cctgactttt
     1141 aatcagggtg tcccgcgttc gagtcgcgga tagatcacca aaaaggcggc gacatagcca
     1201 agtggtaagg cagtggactg caactccttg atccccagtt cgaatctggg tgtcgcctcc
     1261 aataaaatgc gctcatagct caactggata gagtgtctga ctacgaatca gaaggttagg
     1321 ggttcgagtc cctttgggcg caccatatga atagcgaggg attatagctc agctgggaga
     1381 gcacctgcct tacaagcagg gggtcacagg ttcgagccct gttaatccca ccagtgcaaa
     1441 cctctagtaa aactactaga ggttttattt ttgattgaaa aagtttttat atttaggagg
     1501 taagaaattt gtatatagta ataggaaatg aaatagttga tagtgaagaa cttaaaataa
     1561 ttatagatga aaatagtaaa tttaaagttg aaaaagattt aagtaaaagc acaaaaagag
     1621 aagatgtaat agcatatcag ttaagtatag atttaaatta cctagattct cttataaatg
     1681 agcaatgtaa tttgtccagt ttatctgatg aagagaaatt tgatgaatat atgactttgt
     1741 ctgatgaact tgcactggat ttagaagagt taatgccaaa atatacaata ataaatgcta
     1801 gagcatataa attagacgag gtagatggaa ttgtaaaaat aatactagct gtagcgtatg
     1861 cagatttagg gcacttaaaa ctttctgatg tagtaaagag gctatctaga caagtagatt
     1921 aggatatgaa tatgaactat attaaaaaaa tgtataaaat cttacgtaaa gatgaaaata
     1981 attatgtaaa catagaaaaa aacagtataa aatcaagtca attgtagaaa ttttcacata
     2041 aattcatctg aaattctata aaaaatgcct taattttaat gaggtaaatc gagtttacac
     2101 agtctaaaaa ttacttatat ttataaattt atcttattaa ttaaggaact ttcttaaaca
     2161 tcttcaaaaa taaagtattt tagtctataa tctttaatat tacacaaaca ataaaggaga
     2221 agaatagagt atgcttaaaa agtttaataa aatactagtg gctaatagag gcgaaatagc
     2281 tataagaata tttagagcct gttcagaatt ggggataaaa agtgttggta tatacagtaa
     2341 ggaagataag tatggattgt ttagaacaaa agcagatgaa tcatatttga taggagaagg
     2401 taaagggcca atagatgcat atttagatat ggatggaata atagacctag caaagagaaa
     2461 aaaagttgat gctatacatc caggatatgg ttttctagcg gagaatgcag aatttgctcg
     2521 taaatgtgaa gagaatggaa ttacttttat aggaccatca tctaaggtaa tgaatatgat
     2581 gggggataag attaactcta aaaaaatcgc aaaagaagtt aatgttcaaa ctataccagg
     2641 agtagaaaaa gcaataagaa gtactgaaga agcaaaggaa gttgcaaaca aaataggata
     2701 tcctgttatg ataaaagcat ctaatggcgg tggcggtaga ggtatgagaa ttgtacatcg
     2761 cgaagaagac ttagaacttg aatatgaaac tgcttgtagt gaatcaagaa aagcttttgg
     2821 agaagatatt atatttatag aaaaatatat agctgatcca aaacatatag aagttcaaat
     2881 attaggtgat aattatggaa atatagttca cttatatgaa agagattgtt cagttcaaag
     2941 aagacatcaa aaaataatag aatatgcacc agctttttcg ttagatgata aagtaagaaa
     3001 agagatatgt gaagatgcag ttaagttatc aaaacatgtg ggttattcta atgcgggaac
     3061 attagaattt ttagtagatg caaatggtgg gcattatttt atagagatga acacaagagt
     3121 acaggttgaa catactgtaa cagagatggt tacaggaata gatatagttc aaagtcaaat
     3181 actaatagcc caaggttaca gcttagatag tgaagaaatt aatataaaat cacaagatga
     3241 tgtggaaata agaggttact ctatccaatg tagaataact acagaagacc ctaagaataa
     3301 attcatgcct gatacaggta aaattcaagt ttatagaact ggttcaggat ttggtataag
     3361 actagatgga ggaaatggat ttacaggggc aaacataagt cctcattatg atagtttatt
     3421 ggttaagaca atatcttggg atagaacatt ccaaggagca ataaataaaa ctataagatc
     3481 tataaaagaa cttagagtta gaggtgttaa aactaatgta ggattcctag ttaatgtttt
     3541 aaataatcct atattctcaa atggtaaatg tagtactaag tttatagatg aaaatccaga
     3601 tttatttgaa ataacagaaa gtaaagacag aggaacaaaa ttacttcaat ttataggaga
     3661 tgtaattgta aatgataatg cttgtaaaga aaaaccacta tttgatgcat tacatgaccc
     3721 aagaatggat aaggatggtt caaaatcaga aggaagtaaa atacttttcg ataagttagg
     3781 taaatctgca tatatagaaa agataaaaaa tgataagaaa ttactattaa cagatacaac
     3841 aatgagagat gcacatcaat cattattagc aacaagaata agaacatatg acttattaaa
     3901 agctgctaag ccaacagaaa aatatcaaaa ggatttattc tcacttgaaa tgtggggagg
     3961 agctacttat gatgttgcat atagattttt aaaagaatct ccttggcgta gattacaaaa
     4021 gctaagagaa gaaataccaa gcataatgtt ccaaatgttg cttagagcat caaatggagt
     4081 tggatataaa aactatccag ataatgtaat agaagaattt acaaaagaat ctgctagaca
     4141 aggtatagat gtatttagaa tatttgattc attaaactgg gttgaaaata tgaaaccatc
     4201 tattaataca gctttagaaa ctggaaaaat tgttgaagca actatgtgtt atacaggtga
     4261 tatactagat aagactaaaa ctaaatacaa tctagaatat tatattaaaa tggcacaaga
     4321 attagaaagc ttgggagcag atataatagc aataaaagat atgtcaggtc ttcttaaacc
     4381 atattcagct tacacactag ttaaagagtt aaagaaaaat gtaaaagcac caatacattt
     4441 acacactcat gatacaagtg gaaatggtgt agcaacttgt ttaatggcgt cagaagctgg
     4501 agtagacata attgatgctg cattagaatc tatggcagga cttacaagtc agccatcgct
     4561 taatgctata gtagaggcac ttaagaatac agagagagat acaggaatag atttatttgg
     4621 atatgatgag ttaggaaaat attataagga tttaagaaaa gtttataata aatttgaaag
     4681 tgatttaact aattcttgtg cagaaatata taattttgaa ataccaggag gccaatatac
     4741 aaacttaaaa cctcaagctg atagtttagg attagttaat agatttgatg aagttaaaga
     4801 aaaatataaa gaagctaatg aagttgtagg agatataata aaagtaactc catcttcaaa
     4861 agttgtagga gacttagcta tctttatgac taaaaataaa ttagataaag acaatataat
     4921 agaagaaggt aaaaatttat cattcccaga ttctgttgtg gattactgta aaggtatgat
     4981 tggtcaacca gaaggtggaa taccaaaaga tttacaagag gtagttctaa aaggtgaaga
     5041 agctataaca gtaagaccag gttcattatt accagcagaa gattttgatg aaatagctaa
     5101 atatctaaat gaaaagtatg atataaatgc taatataaga aatgttataa gttatgcttt
     5161 atatccaaaa gtatatgaag attatatcaa acatctacaa cattataatg atatatctaa
     5221 acttgaaagt gatgtattct tctatggatt aaataaaaat gaagaatgtg aagtagaaat
     5281 agaagaaggt aaggtattaa caataagatt agttgaaatt ggtgaagtta aggaaaatgg
     5341 atttagaact ataggatttg agttaaatgg aatggttaga gaagtagaaa taaaagataa
     5401 gaatttctct ggaaaaatta ataatgttga aaaagctgat atgaatgacc ctcttcaaat
     5461 tggagcaagc ataccaggaa aagttataaa aataatggtt aaagaagaag atgaagtaaa
     5521 agcaaatcaa ccattaatcg ttatagaggc tatgaagatg gaaactataa tagttgctaa
     5581 gactgatgga gtaattaagt caatcaaagt aaaagaagat gatatggttg aagataaaca
     5641 attattaatg ataatgaagt aatttaatta aaataaatta aaaagcactc tgaaatgttt
     5701 aataaaaaat gttttagagt gtttttattt attttaatta aatatttgtt aaaaaaaaga
     5761 ggacaattac ctccagatgt agaaataaag ctagtaagga aaatgttatc taaaaattct
     5821 aaatatagta aatattttcc ataatatttc ttaggaaata acagcaaaga taaaatacat
     5881 aaatttaaaa ggtgggtgct tatatgaagg tttatgatag taaaatgttg agaaatgtag
     5941 cagtattagg acatagcgga tgtggaaaaa caaatctaat cgaaactata gcttacacag
     6001 caaacactaa taagatacct aaattaacag acaaggtaaa tatgacttat agcatgggat
     6061 taatacctat agagtataat gattataagt ttaacttatt ggatacccca ggatattttg
     6121 actttagcgg agacgttgtg tcatctctta gagcaagtga tgcagcaata atagtaatag
     6181 atgcaacagc gcctattcaa gttggtacag agaaatcatt agaactaact gagagtattc
     6241 ctaagataat gtttataaat aaaatagata atgaaaaggc aagatacaaa gatgcaatag
     6301 caatgttaag agaaaaatac aataataaga tagttcctat gataagtcct atatacaaag
     6361 ataaaaactt tgtaaagtta cataatgttt ttgaaaacat agatgattta gaaggtgaat
     6421 ttaaggaaca agctatgagt gttaaagaag cattaatgga acttatagca gaaactgatg
     6481 accaaatatt agataaatat tttaatggag aagaactaac tacggaagag atacaaaaag
     6541 gtattattat aggtatacaa agaggtgata ttatacctgt aatatgtggt tcaactataa
     6601 ataatatagg cacaaaggaa atattagata caatatcaag ttatttagaa cctatcttta
     6661 cagaggaaag caaaccattt aggggattag tatttaaaac tatggttgac ccatttgttg
     6721 gaaagatgtc atatataaag attactgagg gtgtactttc taaggataaa gatgttttta
     6781 atataaataa aaatgtaaaa gaaaaaatag ctaatatata tacattgaga aatagtgaat
     6841 tagtagaaat agaaaaagct aaagctggtg atatagtagt tataactaaa gttaattctc
     6901 taaaaacagg agacactata tctgcagata aagatgcaga agctttagaa aagatagatt
     6961 ttcctaaacc acaaatatat tatgcagtaa ctcctaagaa taaaggtgat gaagaaaaag
     7021 tagcatctgt attaaataaa ctagtagaag aggaccctac attacattgg tatagaaata
     7081 cagagacaaa acaagcactt ttaggaggtc aaggagaact tcatataaaa acaattaaaa
     7141 ataaaatgaa agataagttt ggtgttgatg ttgaattgaa tgaccttaaa gttccttata
     7201 gagaaactat aaaaggaaca gccgatgtac aaggaaaaca taaaaagcaa tcaggtggtc
     7261 atggtcaata tggagacgta aaaataagat ttgaaagatg tgaatctgat tttgaattta
     7321 cagaagaaat atttggtggt tctgtaccaa aacaatatat accagcagtt gaaaaaggtc
     7381 taaaagactc tatgcaaaaa ggtatattag caggttatcc tgttacaaac ataaaagcta
     7441 cgctttatga tggttcatac catgatgtag actcttctga aatggccttt aagatggcag
     7501 caagtgcagc ctttaaaaaa ggtatggaag aagcgcaccc aatactacta gaacctataa
     7561 tgaaacttaa gataactgtt ccagaagaat atatgggaga tgtcatgggt gatataaata
     7621 agagaagagg aaaaatattt ggaatggaac cagatgacaa agggaaacaa ataatatttg
     7681 cagaagctcc acaagcagag acatttaaat atgcaataga tttaagagca atgacacaag
     7741 gtagaggata ctttgaaatg gaattagaga gatatggaga ggttccatca cagtttgcag
     7801 aaaaaataat tgggttagct actgcaaagt aatgaacaaa ataaatagaa aaataattaa
     7861 tatatattat taaaaatatt tttagggttt taactaggta taggtttatg ttaggtaaac
     7921 ttatacctag tttttata
//
LOCUS       DAMJWP010000164         7856 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.170, whole genome shotgun sequence.
ACCESSION   DAMJWP010000164 DAMJWP010000000
VERSION     DAMJWP010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7856
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.170"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(81..761)
                     /locus_tag="PC700_002793"
     CDS             complement(81..761)
                     /locus_tag="PC700_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2582072.1"
                     /translation="MIHLMKLELKKVGLKRYFLFSILGILLCMFFVFTGLNDKSTSIY
                     DYDVTFRTISLVFCFYYIILFAVLNVAYIINEYTNKTILILFSYPLDRKKLILSKLLL
                     ITTLMISSMIVGYICCFTFVVVLDKYFNLVIGDFHISILSYWIPGAIKSIIVFCSLGI
                     WTFVVGMIRKSIPVTLVSSIIFINLRQFILAGTNTTQDSLISVFIIVGITVIGIYYIL
                     NHKVTEID"
     gene            complement(758..1684)
                     /locus_tag="PC700_002794"
     CDS             complement(758..1684)
                     /locus_tag="PC700_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2582073.1"
                     /translation="MNYIIETHQLKKIYKDKAVVNDVNIHVKKGEIYGFVGPNGAGKS
                     TVMKMLLNLVKPNSGEIVMFSKKVAETDFEILKKIGTIIENPYFYENLTAKQNLDLHC
                     EYMGYYNKEHISEVLECVGLSKQSNKKVSKYSLGMKQRLAIARAILTKPELLILDEPI
                     NALDPEGIREMRELFRKLNTDYGITIFISSHILSEVEQIADTIGIIQDGKLIKEISMS
                     DIHKYQTDYVEVDVDNVELAGYLLEKEFGIKDFKITSESCIEIYDLRKDVKEISKIFI
                     KNQIGINSIGRKQSSLEDYFFQTTGTGGIETL"
     gene            complement(1757..2674)
                     /locus_tag="PC700_002795"
     CDS             complement(1757..2674)
                     /locus_tag="PC700_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2582074.1"
                     /translation="MELFLVVISIVLFAFYLKERNKSKKMESDYKYINSRLRDITNDD
                     EINYILVPSDISIVKETAVDINKLLEKFYSRQINYNRSEKAILQIFTNISHDLRTPIT
                     VLKGYIEMLYLQSQKEELSSSMRATLEKMQTNSNELVNSINNLFNMAKIQSGDMILNL
                     QKSNLTQLCHEIILEFYDLLDKENFNVEVNIEDRPIYANVDTEALQRILKNLIDNAIK
                     YGSDGRFLGISLYEKEKHVFIDIEDKGSGISETEKEHIFTRAYTADRKKGNGLGLAIS
                     QGLANSMGGSIYVNSRPNVKTVFTVKLKS"
     gene            complement(2674..3363)
                     /locus_tag="PC700_002796"
     CDS             complement(2674..3363)
                     /locus_tag="PC700_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2582075.1"
                     /translation="MDILLVEDDKNIREMVYDFLISENYFVKTAVDGNTAIEIFKNYS
                     FDIILLDLMLPKSSGFEVLKYIRSVSVVPVIITTAKDSDTDKMLGLNLGADDYITKPF
                     SIVELLARIKANIRRATVYSSSRNSEDMVIQYKDLIINISKYVVSKNGIDLNLTHTEF
                     EIVKLLATNQGRAFSKEQLYNTIWKEPYYGNENVLNTHINRLRNKLSDGIEKNNYIKT
                     LWGIGYKMEEE"
     gene            complement(3771..4076)
                     /locus_tag="PC700_002797"
     CDS             complement(3771..4076)
                     /locus_tag="PC700_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582076.1"
                     /translation="MPSMIMEVVGVLFIAIGLILTFIEIKGNFPSFYGFGQNSIGKNA
                     VGIIAAGIALIFFRNGIDSIDSIATYGVFLAIELVVIVAYFLIRKKFTKEDKHLRTN"
     gene            complement(4798..5016)
                     /locus_tag="PC700_002798"
     CDS             complement(4798..5016)
                     /locus_tag="PC700_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2582077.1"
                     /translation="MSVLLKTKIKSYREKNLMTQGELAKSVGVRRETIVHLENGRYNP
                     SLKLAMDIAKVFDTTVEELFEFIEENNI"
     gene            complement(5017..5424)
                     /locus_tag="PC700_002799"
     CDS             complement(5017..5424)
                     /locus_tag="PC700_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582078.1"
                     /translation="MSKQQTGIKSTLISEILGISYCIGATLVNKYFFKGSSIEYLPII
                     IWVLAMGAQWVYNKKYDDLIDEASKCILSRVNDIAIKVLFFSVAIVSIFLVTLDINVS
                     NLNIGMSLLVILFIQSLLKLILFIYFDRKGIYN"
     gene            complement(5510..5998)
                     /locus_tag="PC700_002800"
     CDS             complement(5510..5998)
                     /locus_tag="PC700_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HDF2582079.1"
                     /translation="MKFYTIQTLEFWNNNKNNKYLSNDYSFILEDFDIPYKWMYSQMT
                     NRIYEFDDSMIWVWTKRPDLRCSGYLEKGKKGVLLEIEISEDNVLLSEFDLWHLPLMD
                     ITVELYEDEQIEKKVSWERIFDLELCSKLNEVNIEDLTLQGVTSKVPLSNVKLIKEFV
                     AK"
     gene            complement(6178..7086)
                     /locus_tag="PC700_002801"
     CDS             complement(6178..7086)
                     /locus_tag="PC700_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDF2582080.1"
                     /translation="MTSENVGEVVTEDGYAKPEYDEVFKSLFTLNNDYDLLANFINDI
                     LKDAPYANRNIKPFTHIKRIIKAETDPTINYIGEKRPRLYILAEDEESNHINIEMQRA
                     LEDDYLERAEYYLSRVHGRKLEEGKEYKEIGKTVGIHILNHVKYNHIEDYVNCLRLTM
                     DGHPNIYSSKTALYFIELPKIRKSSCIANRVLIWGKLIDNPSHIDIRILSKTDYVIKR
                     ALDRLKELGSNEDYLLNLKRGAYIMNKSRNFKEEIFQEGVEKGKREEKFNIIIKLKNK
                     GYNLSEICDIIDDLNKSEVEKIYNQN"
     gene            complement(7637..7807)
                     /locus_tag="PC700_002802"
     CDS             complement(7637..7807)
                     /locus_tag="PC700_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582081.1"
                     /translation="MDSNNNTIKSTVKKGISFGSCLAMIISYTAWKSIPWAIFHGLMS
                     WIYVLYYWVKYA"
BASE COUNT         2687 a         1269 c          757 g         3143 t
ORIGIN      
        1 attaataata gctgatataa tagcaaatca ttattatccc aagaagatac aattaataac
       61 taccttccta tccacagtat ctagtcaatt tcagtaactt tatgatttag tatataatat
      121 ataccaatca cagttattcc aactataata aaaacagaaa tcagactgtc ttgagttgta
      181 tttgtaccag ctaaaataaa ttgtcttaaa ttgataaaaa taattgaact gacaagggta
      241 acaggtattg attttcttat cattccaaca acgaatgtcc atatacctaa tgagcaaaat
      301 acaataatac ttttaattgc tcctggtatc caataagata aaattgaaat atgaaaatct
      361 ccaataacta aattaaaata cttatccaac acaacaacga atgtaaaaca acagatatat
      421 ccaactatca ttgatgatat cataagagtc gtaatcaaaa gtaactttga taaaataagt
      481 tttttacggt caagtggata agagaataaa atcaagattg ttttatttgt atattcattt
      541 attatatatg ccacattcaa cacagcaaat aatataatat agtaaaaaca gaaaactaaa
      601 ctgattgtcc taaatgtaac atcataatca tatattgaag tagatttatc attcaatcca
      661 gtaaagacaa aaaacataca taacaaaatc cccagtattg aaaataaaaa atatcttttt
      721 agcccaactt tttttaattc tagtttcatt aaatgtatca tagtgtttct atacctcctg
      781 tacctgtagt ttggaagaag taatcttcta aagaactttg tttacgtcca atgctattta
      841 taccaatttg atttttgata aatattttgg atatttcttt tacatctttt cgtaaatcat
      901 atatttcaat acaactttcc gaagtaattt taaagtcttt tattccaaat tctttttcta
      961 acaagtatcc agcaagttcc acattatcta catctacttc aacataatct gtttgatatt
     1021 tgtgtatatc tgacatagaa atttctttaa taagtttacc atcttgaata attccaatag
     1081 tgtccgcaat ttgttctacc tctgaaagaa tatgactaga aataaatata gttattccat
     1141 aatctgtatt taacttacgg aataattctc gcatttctct tatcccctct gggtcgagtg
     1201 catttatagg ttcatcaaga atcaataatt ctggtttagt aagaattgct ctagctattg
     1261 caagacgttg tttcatccct aaagaatatt ttgaaacttt cttgtttgat tgctttgaca
     1321 atccgacaca ttccaatacc tctgatatat gttctttgtt ataataaccc atatattcac
     1381 agtgtaaatc aagattctgt ttagcagtca aattctcata gaaataagga ttttcaataa
     1441 tagtaccaat cttttttaat atttcaaaat cagtttctgc cactttctta ctgaacataa
     1501 ctatttctcc agaatttggc ttaacgagat ttagcaacat tttcattact gtacttttac
     1561 ctgctccatt tggtccaaca aatccataaa tttctccctt tttcacatga atattaacat
     1621 cattaacaac agctttatct ttatatattt tctttaattg atgggtctct atgatatagt
     1681 tcatcatact ccctcctttc taaaaacagt atacaaaata aaattatctt ttctctcttt
     1741 taaatctaac ttttttctaa cttttcaatt ttaccgtaaa aactgttttt acattaggtc
     1801 tactatttac ataaattgaa cctcccatag agtttgcaag cccttgagaa atagccaaac
     1861 ctaaaccatt gccttttttt ctgtcagcag tataagctct tgtgaaaata tgctcttttt
     1921 cagtttcaga aatacctgaa cctttatcct caatatctat aaaaacatgt ttttcctttt
     1981 catatagtga aatccctaaa aatctaccat ctgaaccata ttttatagca ttatcaatta
     2041 aatttttgag tatacgttgt aatgcttctg tgtcaacatt tgcatatata ggtctatctt
     2101 caatattaac ttcaacatta aagttttctt tatctagtaa atcataaaac tctaaaataa
     2161 tctcatgaca taattgtgtt agattggatt tttgaagatt taatatcata tctccagatt
     2221 ggatttttgc catattaaac aggttattta ttgaattaac aagctcattt gaatttgttt
     2281 gcattttttc caaagttgct ctcattgaag aagataattc ttctttttga ctttgaagat
     2341 atagcatttc aatataaccc tttaatactg tgatgggagt tcgtaaatcg tgtgatatgt
     2401 ttgtaaatat ttgcaatatc gctttttcac ttctattata attaatttgc ctactgtaaa
     2461 acttttcaag aagtttattt atatctactg ctgtttcttt tacaatcgaa atatcagatg
     2521 gtactaaaat ataattaatt tcatcatcat ttgttatatc acgaagtctt gaattgatat
     2581 acttataatc actttccatt ttctttgact tatttcgttc ttttaagtag aaagcaaata
     2641 gtacaatcga aattacaact aaaaataact ccattattct tcctccattt tgtagccaat
     2701 tccccaaaga gttttaatat aattattttt ttctatacca tcagacaatt tatttcttaa
     2761 cctgttaata tgagtattta gcacattttc atttccataa taaggttctt tccaaatagt
     2821 gttgtataat tgttcctttg aaaaagcacg tccttgatta gtcgctagta atttaacaat
     2881 ttcaaattct gtatgtgtta agtttaagtc aattccattc tttgaaacta catattttga
     2941 tatgtttata atcaaatctt tatactgaat aaccatatct tcagagtttc ttgaactgct
     3001 atacaccgtt gcacgtctaa tattagcttt aattcttgca agcaattcta cgattgaaaa
     3061 aggctttgta atataatcat ctgcaccaag attaagccct agcattttat ctgtgtcact
     3121 atctttcgca gtagtaataa taacaggaac aacactcaca cttcgtatat attttaatac
     3181 ttcaaaacca ctagactttg gtaacatcaa gtccagtaaa ataatatcaa atgaataatt
     3241 tttaaatatt tcaatagctg tatttccgtc aacggcagtt ttaacaaaat aattctcact
     3301 aatcagaaaa tcgtatacca tttctcgaat attcttatca tcttcaacca ataagatgtc
     3361 cattattatt caactccttt tcgtcagtat acttcaaatg atatatttat taaaaagggt
     3421 tgtcaataaa taactgctta tgatattgta tctgcaattt ggtgatactc ttctaagtta
     3481 aagttctttt caataagttt tattgaaaaa ataattaaaa tatagtatac tctcttatct
     3541 ttatctgaaa tatacataat tttcttttat taacatctac aattaactat tttatattat
     3601 aaaacacctt ttaacttttg ctaaaaaata ttttatacat aaaaaagaaa ccacattcta
     3661 gttttataca gaatatagtt tctttaaaaa taatcaaatt gggattttaa ataattaaaa
     3721 acaaactttt atttgttttt aattttccaa ttttatagta gataatttag ttaattagtt
     3781 cttaaatgtt tatcttcttt agtaaatttt tttcttatta agaaatatgc aactattaca
     3841 acaagttcta ttgctaaaaa tacaccataa gtagctatag aatctatgct atctatgcca
     3901 tttctaaaga aaattaaagc aattcctgct gctataatac ctacagcatt tttccctata
     3961 gaattttgac caaaaccata aaaagaaggg aaatttcctt ttatttcaat gaatgtcaag
     4021 attaatccaa tagctataaa tagtactcct acaacttcca taatcatgct aggcattttg
     4081 acccctcctt attaataatt aactactact ataaaaatat ttattatttt tataattata
     4141 ttatatatgc gattacatgt ttttggaagt atatttggaa gtaatttcat atttatttaa
     4201 cttaattttt ttgtgataaa ataattctaa ttttcatata gatattatta taaattttct
     4261 agtttatgga gttattcatt ctataattca caaatatgta ttttttaatc tattaaagta
     4321 ttcattttct ggcttataag catggacttc ttatctataa gcttggtttt tatttataat
     4381 taattcattc tctaatctat aagcttggtt tttatttata agtcatttat tctctaatct
     4441 ataagcttgt tttttaccta taattaattc attctctaat ctataagctt tctttttatt
     4501 tataagtcat ttattctcta atctataagc ttacttctta atctataaat cattcatttt
     4561 ttaatctatt agcttgtttt ttacctataa ttaattcatt ctctaatcta taagctttct
     4621 ttttcatcta taaatcattc attctttaat ctatgagctt ggtttttcat ctataagtca
     4681 tttattctct aatctataag cttggttttt tacctataat taattcattc tctaatacat
     4741 aaacttgaat tttttatcta ttaattcatt ctctaatgca taagtaatac atgaaattta
     4801 aatattattt tcttcaataa attcaaacaa ctcttctaca gttgtatcaa ataccttagc
     4861 aatatccatt gccagtttta aagaaggatt gtagcgacca ttttctaaat gtacaatagt
     4921 ttcacgtctt actcctactg actttgcaag ttcaccttgt gtcataagat tcttttctct
     4981 ataggattta atttttgtct ttagtaatac tgacacctaa ttgtatatcc cctttctgtc
     5041 aaaatatata aatagaatta acttcaacaa ggattgaata aacaaaatga ctaataaaga
     5101 cattcctata tttaaatttg acacatttat gtccaaagtt acaagaaata tagatactat
     5161 agctacagaa aaaaacaata cttttattgc aatatcatta actcttgata gtatacattt
     5221 tgatgcctca tcaattaaat catcatactt tttattatag acccactgag ctcccatagc
     5281 taatacccaa ataattatag gaagatattc aatactactt cctttaaaaa aatacttatt
     5341 tactaatgta gctccaatac aataagaaat tcccaatatt tctgaaataa gtgttgattt
     5401 aataccagtt tgttgtttag acatataaaa tcccccttaa aaagttatgt atttataaca
     5461 cctcttgtta taaatatata acttttttga ataaatttca ataaaaatat tattttgcaa
     5521 caaattcttt tattaatttt acattagata gaggcacttt agatgtcact ccttgcaatg
     5581 tcaaatcttc tatattaact tcatttaatt ttgaacacag ttctaagtca aatattcttt
     5641 cccaacttac cttcttttca atttgttcat cttcataaag ctctacagta atatccatta
     5701 aaggtaaatg ccataaatca aattctgata ataacacatt gtcttcactt atttctattt
     5761 caagtaaaac accttttttt cctttttcta aataaccact acatcttaaa tcaggtctct
     5821 tcgtccaaac ccatatcata ctatcatcaa attcatatat tctatttgtc atttgactat
     5881 acatccattt gtatggaata tcaaagtctt ctaatataaa agaataatca tttgatagat
     5941 atttattgtt tttattgttg ttccaaaatt ctaaagtctg tatagtataa aatttcatat
     6001 ataaatcaac ccctttgttt ttctataaga aggagatagt tcaaactttt attttgaatc
     6061 atctccttag cttatatatc tctttgtatc tactatagtt aaataacaag gttttatatt
     6121 aatttaaatt taaaagagga agctttatat ttatctagtg ccatctcaat tatagtatta
     6181 gttttgatta tagatttttt caacctctga tttattcaaa tcatctataa tatcacatat
     6241 ttcactcaaa ttatatcctt tattttttaa ttttatgatg atattaaatt tctcttctct
     6301 tttccctttt tcaacacctt cttgaaaaat ttcttcttta aaatttctac ttttattcat
     6361 aatatacgca cctctcttta aatttaataa atagtcctca ttactgccta attcttttaa
     6421 tctatcaagt gctcttttaa taacataatc tgttttagat agtattctaa tatcaatatg
     6481 agaaggatta tcaataagtt taccccatat tagaactctg tttgctatac aactggattt
     6541 acgaattttt ggaagttcta taaaatataa tgcagtttta ctagaataaa tatttggatg
     6601 cccgtccatt gttaatctca ggcaatttac ataatcttct atgtgattat attttacatg
     6661 atttagtata tgtattccaa cagtttttcc aatttcttta tactctttac cttcttctaa
     6721 ctttctacca tgtacacgac taagataata ttcagctctt tccaaataat catcttcaag
     6781 agctctttgc atctcaatat ttatatgatt actttcttca tcttcagcca aaatataaag
     6841 tctaggtctt ttctcaccaa tgtaattaat tgttgggtcg gtttcagcct taattattct
     6901 ttttatatga gtgaacggtt tgatattcct attggcatat ggagcatctt tgaggatatc
     6961 atttataaaa tttgcaagta aatcgtaatc attatttaaa gtaaacaatg atttaaatac
     7021 ctcatcatac tcaggcttag catacccatc ttcagttaca acttctccaa cattttcact
     7081 agtcaatatt tcttttggaa ttaaatcctt gattttatct aatttttcat tagaaaaatc
     7141 acctctacta agtatgtcat ttaatttttt ggttcattct ctgtcctttt cttcaattat
     7201 agttcgttta tactcttttg atgttttaat aatcttagat ttttcttcta atttttcttt
     7261 catatcaaca cctctttttt attattaccc atttcttcat accaaagtct gaacttctaa
     7321 gtaaaatttg cattattgaa ataagaacct tataaactta tatatcatta ctaaagttaa
     7381 gactttaaag agaagcactt agattacaat agattttaga tagctttctt tttaaaacta
     7441 ttgttgataa atgaatgaat ataagggtat gtattaaaat agagatactc atctttttca
     7501 ttgtttaagt aatgaaaata tattttattt aatacaaaaa agagtgcctt ataaactaaa
     7561 aagttcactt cgagacactc ccttacattt tgtttcttaa actttacaat attacatact
     7621 tatttattaa tttaatctat gcatacttaa cccaataata aagtacatat atccaactca
     7681 ttaagccatg aaaaatagcc catggaatag atttccatgc agtataagaa ataatcattg
     7741 ctaaacaaga accaaaagaa ataccctttt taacagttga ttttatagta ttattatttg
     7801 aatccataat taatcctcca aaattcaaaa taaacaaatt attatatatc ttattt
//
LOCUS       DAMJWP010000165         7847 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.171, whole genome shotgun sequence.
ACCESSION   DAMJWP010000165 DAMJWP010000000
VERSION     DAMJWP010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7847
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.171"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            7..771
                     /locus_tag="PC700_002803"
     CDS             7..771
                     /locus_tag="PC700_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaP domain-containing protein"
                     /protein_id="HDF2582082.1"
                     /translation="MQIIDININNIDSRHICCSISEKKGENCVFSKKAWMKNNFDKGL
                     AFKRFDVRGKAFIEYIPAENAWCPITADNYIFINCFWISGKFKGQGYSNLLLQECVDD
                     AKSKGKYGLVVLSSSKKMPFLSDAKYLKYKGFCLADTSEPNYELLYFPFTDNAPIPCF
                     KNCVKKRKLNENGIVLYYSNQCPHTEKYAPILKKIANSRGIDVNLIKFETAKEAQNAP
                     APFTTYSLFFNGEFVTNEILSEKKIEKFLDTNIKKI"
     misc_binding    994..1207
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1461..2495
                     /locus_tag="PC700_002804"
     CDS             1461..2495
                     /locus_tag="PC700_002804"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HDF2582083.1"
                     /translation="MNKIKVGIIGATGYVGAELIRLLMNHDKVEVTAIGSNSYVGKNI
                     VDIYPSIGYKNNMICIENEKVIDMCDVVFTALPHGVSEKFVIKAIKSKKKVIDLGADF
                     RIKDEEVYSKWYGVSFIDKILHKKAVYGLSEIYKEDIKDADIIANPGCYPTSISLPLM
                     PLLSSKLIKNNNIIIDSKSGLTGAGRELSISSHFTEVNENITAYKIGKHRHTPEIEQN
                     LSESCKEKVSVVFTPNLIPVNRGILSTIYCTKEDNISINDIHRKLTDYYEFKEFIEVL
                     PLDKVASLKNVRFSNKCVISLHENGDTLIICSAIDNMIKGAAGQAIQNMNIIFGIEEN
                     TGLKNIAPSF"
     gene            2537..3754
                     /gene="argJ"
                     /locus_tag="PC700_002805"
     CDS             2537..3754
                     /gene="argJ"
                     /locus_tag="PC700_002805"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HDF2582084.1"
                     /translation="MEIIKGGVTVSEGFFASGIHCGLRKNKEKRDLALVYSDVLCDAA
                     AVVTQNKVKGNPVYVTQEHIKNGKAQAIIVNSANANTFNGKEGLINAYKMAKFTSDKL
                     NIKENDVLVASTGVIGKPLNINLIEENMDELVNNLSKQGHIGAREAIMTTDIIKKEIA
                     VAIMYGDKKITIGGMAKGSGMIHPNMATTLGFITTDANIDGVLLKEALKIAVDKSFNR
                     VSVDGDTSTNDMVMILANGKAKNDRINKKDEHYQVFLSALTYVCIELAKLVAKDGEGC
                     TKLIECYINGALSEEHAVKLAKTVISSSLVKTAVFGADANWGRILCALGYAGEEIDME
                     KVDIIFESMKGYIEVCKNGNGLDFNEEKAKKILEDDEISILVDLNMGNARGNAWGCDL
                     SYDYVRINGSYRN"
     gene            3769..4629
                     /gene="argB"
                     /locus_tag="PC700_002806"
     CDS             3769..4629
                     /gene="argB"
                     /locus_tag="PC700_002806"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HDF2582085.1"
                     /translation="MINIEKANTLIEALPYIEKHQGKTIVVKYGGSAMKKDGLKESVM
                     EDLVLMSYVGINIVLVHGGGAEINKMLAKVDIESKFVNGLRYTDEETMEIVKMVLAGK
                     VNKDLVNKIHTKGGKAVGLCGMDNNMILCDPYKNYELGFVGEIKKVNVELIESCLKSG
                     YISVIATIGVGDDGETYNINGDTAASAIAKELNADKLILLTDVPGLLREPDEEKSLIT
                     EVILEDVDKLFEEGIITGGMIPKIEGCVDALNNGVNRVHILDGRVPHSIITELFTDSG
                     IGTLIRKENE"
     gene            4659..5846
                     /locus_tag="PC700_002807"
     CDS             4659..5846
                     /locus_tag="PC700_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HDF2582086.1"
                     /translation="MNNNNTISKWNEYFIDTYNQPNFVIDYGEGSCFFDTNGNKYIDF
                     TSGYGVSSLGYSNSNLKNALKEQVDKLLHTSNLYFNEPVLYSGEKIINSSGMAKVYFC
                     NSGTEANETAFKIARKYSSDKYGNGRGTIISLKDSFHGRTMMSLMATGMDKYHKYFYP
                     LPEGFKYIERNNIEDLKNNLDSTVCAIILEAIQGEGGVNVLEKDYVLEIVKICQEKDI
                     VVIFDEVQCGIGRTGKLFGYEYFDVKPDIVTVAKGLGAGIPVGGVLVNKKLSKVLGKG
                     DQGTTFGGNLLAMVAASVVLDEISKDGFYNEVLEKGNYIRKSIESFNNKVVLKTKGIG
                     LMIGIETNIESSIIEEKARKKGLLILTAGKNVLRFLPPLTISYKEIDEALEILKDILL
                     EIN"
     gene            5955..6977
                     /gene="argF"
                     /locus_tag="PC700_002808"
     CDS             5955..6977
                     /gene="argF"
                     /locus_tag="PC700_002808"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDF2582087.1"
                     /translation="MNNISLKGKSFLTLKDFTKEEIRYLLTLSSYLKTKKKVGIKGDL
                     LQGKNIALLFEKTSTRTRCSFEVAAHDEGAYVTYLGPNDCHMGKKESVSDTAKVLGRF
                     YDGIEFRGFKQETVESLAKYSGVPVWNGLTDEYHPTQILADFLTVIENVDKDLNKVKF
                     VYVGDARNNMGNSLMIGCAKMGIHFVALAPKELWPNEELVKEMKEIAKKSKGDIMLTE
                     NIDDVKGADVIYTDVWVSMGEEEQYEARINQLKDYQVNMDMIKKTENENVIFLHCLPA
                     FHDLETKVAQKIYEKFGLSELEVTNEVFNSKYSKVFEEAENRMHTIKAVMVSTMTDVN
                     MNNNLI"
     gene            7104..7721
                     /locus_tag="PC700_002809"
     CDS             7104..7721
                     /locus_tag="PC700_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HDF2582088.1"
                     /translation="MKKIILALTTFLLAISLTACSSAKPEDTIDSFFNSAKKFDFEGM
                     NKVMENNDEKYKDILKELDTKDPNAQYVLDYLKQNASKITYTIKDSEVKGDKATIKVE
                     CKFIDSTPLLQEIVAEAFTKMIGMSFSGQDLTDEKTTEMLVSIMKEKQKSVKETYVTK
                     TVEFECSKKDNKWIISSVNDAVADVLLSNLVTAGQEFSNSMNTLE"
BASE COUNT         3186 a          754 c         1470 g         2437 t
ORIGIN      
        1 ataagtatgc agattataga tataaatata aataatattg acagtagaca tatatgctgt
       61 tcaatttctg aaaaaaaagg agaaaattgt gttttttcta aaaaagcttg gatgaaaaat
      121 aattttgata aaggtctagc ctttaaacga tttgatgtaa gaggtaaagc ttttatagaa
      181 tatattccag cagaaaatgc atggtgtcct attactgcgg acaattatat atttattaac
      241 tgtttttgga tatctggaaa atttaagggt caaggatatt ctaatctatt gctacaagag
      301 tgtgttgatg atgcaaaatc aaaaggtaaa tatggattag ttgtgctttc gtctagtaaa
      361 aaaatgcctt ttttatctga tgctaaatat ctaaaatata agggcttttg tttagcagat
      421 acatcagaac caaattatga gctattatat tttcccttta ctgataatgc tcctattcca
      481 tgttttaaga attgtgtaaa aaaaagaaaa ttaaatgaga acggtatagt tttatattac
      541 agtaatcagt gtccacatac agaaaaatat gctcctattt tgaaaaaaat tgcaaattct
      601 agaggtattg atgtaaatct tataaaattc gaaacagcaa aagaagcaca aaatgctcct
      661 gcaccattta ctacatacag tctttttttt aatggtgaat ttgtaaccaa cgagatactt
      721 tctgagaaaa agattgagaa atttttagat accaacatta aaaaaattta actaaaaata
      781 gatgtaaata ttgtattttg agattacatt attttataat ccaatatatt ttggaaattt
      841 cttaagttga tgaatgataa tataaagatt ataactcaat ttagatacaa aatgctggaa
      901 attaaccata tagaaaatat ttccaaaaat ttaaaaatta tattgacaaa gttaaaaaaa
      961 taaactatca tataaaacaa taagaaaata aattgaaatc ctgtgaaaaa gagagtagca
     1021 taaagctagt tgatagagag ctgggaatgg tggaagccta gtaacgaatt ttattgtgaa
     1081 tagagctttg gaggagatta ccagaagtaa tagtatggta attggtgaat ctcgccttaa
     1141 gagattagag tgggtaagat tatcttatca attagagtgg taacgcgggt gtatattcgt
     1201 ctcttggtct ttgaccaaga ggctttttta attttaaatg tacattttgt agatactttt
     1261 taatttgagt tttaatgatt attgtaattt taccaatgag agtggtaccg cggaaaatcg
     1321 cctcttagta tttataatgc taaggggttt tttgttttaa ttagaatatt ttaatctaca
     1381 tattatcaaa tgattatcat atagtaaagt ggaaagatag aattttaata ctatttgaat
     1441 ataaaaatgg agggaacaaa atgaataaaa taaaagttgg tattattggc gcaactggat
     1501 atgtcggggc agagttgatt agattattaa tgaatcatga taaagtcgaa gtgacagcaa
     1561 taggttcaaa ttcgtatgtt ggaaaaaata tagttgatat atatccaagt ataggatata
     1621 aaaataacat gatatgtata gaaaatgaaa aagttataga tatgtgtgat gttgtgttta
     1681 cagcccttcc acatggagtt agtgaaaagt ttgtaataaa agcaattaaa tcgaaaaaga
     1741 aagttataga tttaggtgct gactttagaa taaaagatga agaagtatat agtaaatggt
     1801 atggagtatc ttttatagat aaaatcttac ataaaaaagc tgtatatgga ttaagtgaaa
     1861 tatacaaaga agatataaaa gatgcagata taattgcaaa tcctggatgt tatccaacta
     1921 gtatttcttt accattgatg ccattactaa gttctaaatt gataaaaaat aataatataa
     1981 taattgattc taaatcaggt cttacaggtg ctggtagaga attatcgatt agtagtcact
     2041 ttacggaggt taatgaaaat atcactgcat ataaaatagg gaaacacaga catactccag
     2101 aaatagaaca aaatttaagt gaatcctgca aagaaaaagt aagtgtagtt tttacaccaa
     2161 acttaatacc agtaaatagg ggaatcttat caactatata ttgtacaaaa gaagataata
     2221 tttctataaa tgatatacat agaaaattaa ctgattatta tgaatttaaa gaatttatag
     2281 aagttctccc attagataag gtagcttctt taaagaatgt taggttctca aataaatgtg
     2341 tcatatcatt acatgaaaat ggagatacat tgataatctg ttcagctata gataatatga
     2401 ttaagggagc agcaggtcaa gctatacaaa acatgaatat aatatttggt attgaagaaa
     2461 atacaggttt aaaaaatatt gcaccatcat tttagtaatg aaaaaatact aaactcatat
     2521 aattaggagg ataaatatgg agattataaa gggtggagtt acagtttcag aaggattttt
     2581 tgcttcaggg attcactgtg gcttgagaaa aaataaagaa aaaagagatt tagctttggt
     2641 ttattcagat gttttatgtg atgcagcagc agtagtaact caaaataaag taaagggcaa
     2701 tcctgtttat gttacacagg aacacattaa aaatggaaaa gcacaagcta tcatagtaaa
     2761 tagtgctaat gctaatacct ttaatggaaa agaaggactt ataaatgctt ataaaatggc
     2821 taaattcact agtgataaat taaatattaa agaaaatgat gttttggttg catctacagg
     2881 tgtcatagga aagccattaa atataaattt aatagaagaa aatatggatg aattagtgaa
     2941 taatctaagt aaacaaggtc atattggagc aagagaagct attatgacaa ctgatattat
     3001 aaaaaaagaa atagcagtag caataatgta tggagataaa aaaataacta ttggaggtat
     3061 ggcaaaaggt tctggcatga tacatcctaa tatggcaaca acgttaggat ttataactac
     3121 agatgcaaat attgatggag ttttgctaaa agaagctttg aaaattgctg ttgataaaag
     3181 ttttaataga gttagtgtag atggagatac aagtactaat gatatggtga tgattctagc
     3241 aaatggaaaa gcaaaaaatg atagaataaa caaaaaagat gaacattatc aagtattttt
     3301 gagtgcactt acatatgtct gtatagaact tgctaaactt gttgcaaaag atggagaagg
     3361 atgtacaaaa ctaattgaat gttatataaa tggagcatta agtgaagaac atgcagtaaa
     3421 attagcaaaa acagttatat cttcttcatt agtaaagaca gctgtatttg gagctgacgc
     3481 aaattggggt agaatactgt gtgctttagg ttatgcaggt gaagaaatag atatggaaaa
     3541 agttgatatt atttttgaga gtatgaaagg atatattgaa gtatgtaaaa atggaaatgg
     3601 attagatttt aatgaggaaa aagcaaaaaa aatccttgaa gatgacgaaa taagtatatt
     3661 agtagattta aatatgggca atgccagagg aaatgcatgg ggatgtgatt taagctatga
     3721 ttatgtaaga ataaatggaa gttatagaaa ttaggaggat atactttaat gattaatata
     3781 gaaaaagcaa atacacttat agaagcttta ccatatatag aaaaacatca gggaaagact
     3841 attgtagtta agtatggtgg aagtgcaatg aaaaaagatg gtctaaaaga atctgttatg
     3901 gaagaccttg tattaatgag ttatgttgga atcaatatag ttttagtaca tggtggaggg
     3961 gcagaaatca ataagatgct agcaaaagtg gatatagaaa gtaaatttgt aaatggtctt
     4021 agatatacag atgaagagac catggaaatt gtaaaaatgg tacttgctgg aaaggttaat
     4081 aaagacctag taaataaaat acatacaaaa ggtggaaaag cagtagggct ttgtggaatg
     4141 gataataata tgattttatg tgacccatat aaaaattatg aacttggatt tgtaggagaa
     4201 atcaaaaaag taaatgttga gcttatagaa tcttgtctaa agtcaggtta tatttcagta
     4261 attgcaacta taggtgttgg agatgatgga gagacttata atataaatgg tgacacagca
     4321 gcttctgcta tagctaaaga gttaaatgca gataaattga tacttcttac agatgtccct
     4381 ggtttgttaa gagagccaga tgaagaaaaa tcattaataa cagaagttat attagaggat
     4441 gttgataaat tatttgaaga aggtataatt actggaggta tgatacctaa aatagaggga
     4501 tgcgtagatg ccttaaataa cggagtaaat agggtacata ttttggatgg aagagttcca
     4561 cattctataa taacagagct atttactgat agtggaatag gtacattaat aagaaaagaa
     4621 aatgaataaa attataagtt taagagggga gaataagaat gaataacaac aatacaattt
     4681 caaaatggaa tgaatatttt atagatacat ataaccaacc caattttgtc atagattatg
     4741 gtgaaggaag ttgttttttt gatactaatg gcaataaata catagatttt acaagtggat
     4801 atggagtatc ttctcttggg tatagtaata gtaatttaaa gaatgctcta aaagagcaag
     4861 tagataaatt attacatact tcaaatttat attttaatga gccagtgttg tattcaggag
     4921 agaaaataat taattcaagt ggaatggcta aagtatattt ttgtaattct ggaacagaag
     4981 caaatgaaac agcattcaaa atagcaagaa agtatagtag tgacaaatat ggtaatggta
     5041 gaggaactat cataagttta aaagactctt ttcatggtag aacaatgatg tctttaatgg
     5101 caactggtat ggacaaatac cataagtatt tttatcctct tcccgaaggt tttaaatata
     5161 tagaaagaaa taatattgaa gatttaaaaa ataatcttga ttccacagtt tgtgcaataa
     5221 ttcttgaagc gattcaaggc gaaggtggag ttaatgttct tgaaaaagat tatgttttgg
     5281 aaatagtaaa aatatgtcaa gaaaaggata ttgtagtgat ttttgatgaa gtccaatgcg
     5341 gtataggaag aactggtaaa ctttttggct atgaatattt tgatgtaaaa ccagatatag
     5401 ttaccgtagc aaaagggttg ggagcaggaa taccagtagg aggagtattg gtaaataaaa
     5461 aactttcaaa agtactagga aaaggagacc aaggtacaac ttttggaggt aatttattag
     5521 ctatggtggc agcttcagtt gtattagatg agatatctaa agatggtttt tataatgaag
     5581 tattagaaaa aggaaattat ataagaaaat ctattgaaag ttttaataat aaagttgtat
     5641 taaagacaaa aggtattggt ctaatgatag gaattgaaac taatattgaa tctagtatta
     5701 tagaagagaa agctagaaaa aaaggattgc ttattttgac tgctggaaaa aatgtattga
     5761 gatttttacc accattaaca atcagttata aggaaataga tgaggctctt gaaatcctaa
     5821 aagatatatt actagagatt aactaaatat tcaaaaaata gttaattaaa gataaaaatt
     5881 agaagtaaat aaaaaagtaa agtgctaaca agtttaattg aatttaagaa gtttataaga
     5941 aggggcgaat taatatgaat aatataagtt taaaaggtaa gagttttttg acattaaagg
     6001 attttacaaa agaagagatt agatatttat taactttatc aagttattta aaaactaaga
     6061 aaaaagttgg cataaaaggc gatttgctac aaggaaaaaa tatagcttta ttatttgaga
     6121 aaacatcaac aagaactaga tgttcatttg aagtagcagc tcatgatgaa ggagcatatg
     6181 taacttatct aggtccaaat gattgtcata tgggtaaaaa agagtctgta tcagatacag
     6241 caaaagttct aggaagattc tatgatggaa tagaatttag aggctttaaa caagaaacag
     6301 ttgaaagtct agcaaaatat tctggtgtac ctgtttggaa tggacttaca gatgaatatc
     6361 atccaacaca aatactggca gattttttaa cagtaattga aaatgtagat aaagatttga
     6421 ataaagttaa atttgtatat gttggtgatg ctagaaataa tatgggaaac tcactgatga
     6481 taggatgtgc taaaatggga atacatttcg tagcactagc acctaaggaa ctttggccaa
     6541 atgaagaatt ggttaaggaa atgaaggaaa tagccaagaa atccaaagga gatataatgt
     6601 tgactgaaaa tattgatgat gttaaaggtg cagatgttat atatacagat gtatgggttt
     6661 ctatgggaga agaggagcaa tatgaagcaa gaataaatca acttaaagat tatcaagtaa
     6721 atatggatat gataaagaaa actgaaaatg aaaatgttat atttttacac tgtcttccag
     6781 catttcatga tttagaaact aaggttgctc agaagattta tgaaaagttc ggattatcag
     6841 aattagaggt tacaaacgag gtatttaatt caaaatattc aaaggtattt gaagaagctg
     6901 aaaatagaat gcatacaata aaagctgtta tggtttctac tatgactgat gtaaatatga
     6961 acaataattt aatatagata tgttttaata tagactttac tttaatgaaa atttataaaa
     7021 tattttaaat atattattca aaaactctca ttatatgtta aaataactta tgtatcaaca
     7081 aattaagagg gaggatttga gatatgaaaa aaatcatatt agctttaaca acttttttat
     7141 tagcaatatc tttaacagca tgttcaagtg caaaaccaga agatacaatt gatagttttt
     7201 ttaacagtgc gaaaaagttt gactttgaag gcatgaataa ggtaatggaa aataatgacg
     7261 aaaaatataa ggatatatta aaggaattag atacaaaaga cccaaatgcc caatatgttt
     7321 tagattatct aaaacagaat gcatctaaaa tcacgtatac aataaaagac agcgaggtta
     7381 aaggtgataa ggctacaatc aaagtagaat gtaaatttat agattcaaca ccattacttc
     7441 aagaaatagt agctgaggca tttacaaaga tgattggtat gagtttttca ggacaagact
     7501 taactgatga gaaaacaact gaaatgttag tatcaattat gaaagaaaaa caaaaaagcg
     7561 ttaaagagac ttatgttaca aaaactgtag agtttgaatg ttctaaaaag gataataaat
     7621 ggataataag ttcagtaaat gatgcagtag ctgatgtatt gttatctaat cttgttacag
     7681 caggacaaga attttctaac tctatgaata cacttgaata atattgttta ggtgtagtaa
     7741 agtaataaat atatgattat ttttataaat aaacatataa atatttatga aggataccat
     7801 ttttggtatc ctttttatta atataatgaa atacatggtt aatttct
//
LOCUS       DAMJWP010000166         7815 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.172, whole genome shotgun sequence.
ACCESSION   DAMJWP010000166 DAMJWP010000000
VERSION     DAMJWP010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7815
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.172"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            167..2479
                     /locus_tag="PC700_002810"
     CDS             167..2479
                     /locus_tag="PC700_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2582089.1"
                     /translation="MDIIKKLSHGCTLDCHDCCKFNVYTKENNVVKIEGDKNHPYTKG
                     FICKKGMAHLDRLNHKDRIKTPMLKVDGVWKEISFDKAIEIMAEKLTYYKEKYTSKSV
                     MHYDQYGSGSVLKYIGDIFFNFYGGVSRHKGGPCWSAGMHAQKYDFGVAKSHAIEDML
                     NSKSIFVWGKNPAYTTIHTMQIIKKAKEKGIKIVVIDPIYTKTAQIADKYVQVNPGTD
                     GALAIAMAKIIVEDKLYDEEYINSYVIGFEEYKKYLSSLELSFLIDECGVKENDIREL
                     VDLYTNKYSSINVGYGLQKYKNGGNTIRAIDALGAITGQIGFSGGGVNYANKVYPSVI
                     NSDPYNSQSYGEDREFYVSNISKFIEESLKNTSNKVNYASDELDTTSNKVNYISDELD
                     TTSNKVNYVSDELDTTSNKVNYVSDELGMALHKVNCVSDELDMTSNKVNYVLDELDIT
                     SNKTDYISNELYNLSNKSIKDNIPIKMAVITKSNMLNQLPDLVELERVFSKIKFKVCF
                     DMFMTDTATLCDMFIPCTNTLESEDIIYSSMTNPYITYNERAVKPAHKLMDEYYFFME
                     LAKKMGLNDYPFVEKRTYLEKVIEPLKRFDKNLDIEKLKNNYFTIHNLVAWEDKKFET
                     PSGKYELYSESIKNLGISPTPVYISNKYKEIEDKNISFRLLTNHHADTLFSQHFMDKK
                     SIAQAYINQRMAKKVGIEDKDIVILRSKKAKINVQINIDDGVGNYIVKMYVGWWKKHG
                     NPNSLTDTGISDFGGQVTYNESMVEIIRQN"
     gene            2567..2902
                     /gene="spoIIAA"
                     /locus_tag="PC700_002811"
     CDS             2567..2902
                     /gene="spoIIAA"
                     /locus_tag="PC700_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HDF2582090.1"
                     /translation="MVNYSLEHKNLYIEFMCSELDHHVANEIREEIDNLLSVNQVKNV
                     VFNFENINFMDSSGIGVIIGRYKKISNEGGRVSVINISSRVKKIFDLSGLNKIIGIYD
                     TYEEALSSL"
     gene            2916..3344
                     /locus_tag="PC700_002812"
     CDS             2916..3344
                     /locus_tag="PC700_002812"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HDF2582091.1"
                     /translation="MNNIMEVKFSAISENESLARVIVASFAAKLDPTVDELVDIKTAV
                     SEAVTNAIIHGYEEDSSKFVFLRCEIEGNTIKIIVEDEGYGIENVEKAMEPLYTSKPE
                     LDRSGMGFTVMKSFMDDVEVSSVKDNGTRIEMTKKINVPK"
     gene            3367..4131
                     /gene="sigF"
                     /locus_tag="PC700_002813"
     CDS             3367..4131
                     /gene="sigF"
                     /locus_tag="PC700_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HDF2582092.1"
                     /translation="MEVTVAREEKKPLLSHEETLELIEKVQNGDEEAKEILISSNLGL
                     VRSVVSRFLNIGYDREDLFQLGSIGLIKSIYKFDPKFNVKFSTYAVPMILGEIKRYLR
                     DDGMIKVSRSLKQIAVKAKMESEALTKKLGREPSIEELAKAIDVEKEDLVMAMEANFN
                     VEYLQGVIHEEEGSPICLIDKISMKGESEEEKVVDNILLKDILGRLDKRERQIIVLRY
                     FEDMTQSEIGEMLNISQVQVSRIEKKVLSKLKEYIS"
     gene            4411..4848
                     /gene="spoVAC"
                     /locus_tag="PC700_002814"
     CDS             4411..4848
                     /gene="spoVAC"
                     /locus_tag="PC700_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HDF2582093.1"
                     /translation="MDKNYKKYVDQISPKPTYLKNYTLAFIVGGIICMIGQAINDLYM
                     KVGGLDKLGASSATSITLIFIGAFLTGLGVYDLIGKRAGAGSIIPITGFANSIVSPAM
                     EYKREGYVLGVGANLFKIAGPVLVYGIGSSILCGIIYYIFKMF"
     gene            5002..6015
                     /gene="spoVAD"
                     /locus_tag="PC700_002815"
     CDS             5002..6015
                     /gene="spoVAD"
                     /locus_tag="PC700_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HDF2582094.1"
                     /translation="MKNKRIGKRTVKLENKPTIISTGTIVGPKEGEGPLKDYFDMIMT
                     DDLYGEKTWELAESKMVETASQQAIQKAGKKLSDVNYMLGGDLINQIVPASFAARELA
                     IPFLGIYGACSTMAEGLCIGSMLIDGGFADLVLSGTSSHYCTAERQFRFPLELGNQKP
                     MTAQWTVTGAGSVLLAPNGDGPKVKYVTVGKVIDEGIDDGNNMGPAMAPAAIDTIYSY
                     FEDTNDDPNSFDIIATGDLGTLGKQIAEDFLKEKGVDISKVYTDCGIEMFNLKEQDVH
                     CGGSGCGCSATVFAGYIYDKLRKKEFNKVMLVSTGALLSPTSTLQKQTIPCVAHAVVI
                     VNE"
     gene            6029..6391
                     /gene="spoVAE"
                     /locus_tag="PC700_002816"
     CDS             6029..6391
                     /gene="spoVAE"
                     /locus_tag="PC700_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HDF2582095.1"
                     /translation="MLMDYVRVFIVGGIICLIAQVMMDYFKMQTPYVMVTYVTTGVVL
                     TFLGLYEPLVKFAAAGATVPIIGFGYSLATGVIKSIQSDGFLGIFLGGTTATAGGIAA
                     AIFFGYIMSVVFTPKAKP"
     gene            6574..7590
                     /locus_tag="PC700_002817"
     CDS             6574..7590
                     /locus_tag="PC700_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF2582096.1"
                     /translation="MVTIKDIARNLGISYSTVSRCLNNNPNVSEKTKKKVVEEANRLG
                     FHFNVNARNLAKKETNRIGVIFSNNFNHQDTRKFFSDIMDSSINSIETNKYDFIIQPN
                     NNISGDSNVYKMVNGQMVDGLVIVSQSIKKEEYDFLKDNNFPHVFIYFKPSFVGEVDN
                     FFWDDNVYGGYIATKHLIDHGHKDIITVTSDDRSSKMHEDRTKGYLNAMEEANLKTEV
                     IKSKMDFESQVEFLEKYIDKIKKASAIFVQQDVPAISIIQELKTTYGINVPEDISIIG
                     YNNIELISYFRSHLSTIDDPREQVIKNGVDSLVNIINKKSTEHIPRKLYPRLIIRNSV
                     KRIK"
BASE COUNT         3136 a          792 c         1441 g         2446 t
ORIGIN      
        1 ttagtatgca aaattctgtg aagcaggaac actgtgactt tagtcatggg aggttcagaa
       61 aatatagcta tgttttatat actgtagttt tgaacataga aagtttaata aattatagta
      121 tatattaatt tttaataagg taagaaagta gaaagaagga tattttatgg atataataaa
      181 aaaattaagt catggatgta cattagattg tcatgattgt tgtaaattca atgtttatac
      241 taaagaaaat aatgtagtaa aaattgaagg agataaaaat catccttata caaaaggttt
      301 tatatgcaaa aaagggatgg ctcatttaga tagattaaat cataaagata gaataaagac
      361 tcctatgctt aaagttgatg gagtatggaa agagatttct tttgataagg ctatagaaat
      421 aatggcagaa aagcttacat actataaaga aaagtataca tcaaagtctg tcatgcatta
      481 tgaccaatat ggaagtgggt ctgtgctaaa atacatagga gatatatttt ttaattttta
      541 tggaggtgtt agcagacata agggtggtcc ctgttggagt gcagggatgc atgctcaaaa
      601 atatgatttt ggagtagcta agagtcatgc tatagaagat atgttaaata gtaaaagtat
      661 atttgtgtgg ggaaaaaatc cagcatatac tacaattcat actatgcaaa taattaaaaa
      721 agctaaagaa aaagggataa agatagtagt gattgacccc atatatacaa aaacagctca
      781 aatagcagat aagtatgtac aggtaaatcc tggaacagat ggagctttag ctatagctat
      841 ggcaaaaatt atagtagaag ataaattata tgatgaagaa tatataaact cgtatgttat
      901 tggttttgaa gaatataaaa aatatttatc ttcattggaa ttaagttttt taattgatga
      961 atgtggtgtt aaagagaatg atataagaga gttagtggat ttatatacta acaaatattc
     1021 aagtataaat gttggatatg ggcttcaaaa atataaaaat ggtggaaaca caataagagc
     1081 gatagatgct ttaggtgcta taactggtca aataggattt agtggtggag gggtaaacta
     1141 tgctaataaa gtttatccaa gtgttataaa ttctgaccct tacaatagcc aatcatatgg
     1201 tgaagatagg gagttttatg taagtaatat cagtaaattt atagaagaat ctttaaaaaa
     1261 tacatcaaat aaagtaaatt atgcatcaga tgaacttgat acaacatcaa ataaagtaaa
     1321 ttacatatca gatgaacttg atacaacatc aaataaagta aattatgtat cagatgaact
     1381 tgatacaaca tcaaataaag taaactatgt atcagatgaa cttggtatgg cattacataa
     1441 agtaaattgt gtatcagatg aacttgatat gacatcaaat aaagtaaatt atgtattaga
     1501 tgaacttgat ataacatcaa ataaaacaga ttatatatca aatgaattgt ataatttatc
     1561 taataagtca atcaaagata atattcctat aaagatggct gtgataacta aaagtaatat
     1621 gctgaatcaa ctgccagatt tagtagaatt ggagagggtg ttttcaaaaa ttaaatttaa
     1681 agtatgtttt gatatgttta tgactgatac agctacttta tgtgacatgt ttataccgtg
     1741 tacaaataca ctagagagtg aagatataat ttatagctca atgaccaatc catatataac
     1801 ctataatgaa agagctgtaa agccagcaca taaattgatg gatgaatatt atttctttat
     1861 ggagctagct aaaaaaatgg gattaaatga ttatcctttt gtagaaaaga gaacttattt
     1921 agaaaaagtg attgaaccac ttaagcgttt tgacaaaaat ctggatatag aaaaacttaa
     1981 aaacaattac tttactatac ataaccttgt tgcttgggaa gataagaagt ttgagactcc
     2041 atctggaaaa tacgagcttt attcagaaag tataaaaaac ttgggaataa gtccaacacc
     2101 tgtatatata agcaataaat ataaagaaat agaagataaa aatatttctt ttagattatt
     2161 aactaatcat catgcagata ctttatttag tcagcatttt atggacaaaa aatctatagc
     2221 tcaagcatat ataaatcaga gaatggctaa aaaagtaggt attgaggaca aagatattgt
     2281 gatattgagg tcaaaaaagg caaaaataaa tgtacaaata aatatagatg atggagtagg
     2341 taattatata gtaaaaatgt atgttggttg gtggaagaaa catggaaatc ctaatagttt
     2401 aacagacact ggtatatcag attttggagg tcaggtaaca tataatgaaa gtatggtaga
     2461 aataatacga caaaactagt taaatttcat tgaaggaata aaaatataat tatagaattg
     2521 attaaaagtt actattttta caaaactatt gaaggaggga tttttgatgg taaattattc
     2581 cttagaacat aagaatctat acatagaatt tatgtgctca gaactagacc accatgtagc
     2641 caatgaaata agggaagaaa tagataactt attaagtgta aatcaagtaa aaaatgttgt
     2701 atttaatttt gaaaatatta attttatgga ttcttcaggt ataggcgtca tcataggtag
     2761 atataaaaag atttctaatg aagggggacg agtttcagta attaatataa gttcacgagt
     2821 taagaagatt tttgatttat ctgggttaaa taaaattatt ggcatttatg atacgtatga
     2881 agaagctcta agttctttgt agggaggagt atgtgatgaa taatattatg gaagttaaat
     2941 tttcggcaat atctgaaaat gaaagtttag caagagttat agtagcatcc tttgcagcta
     3001 agttagaccc aacagtagat gagttagtag atataaaaac tgctgtctct gaggctgtta
     3061 cgaatgctat aatacatgga tatgaagaag acagttctaa gtttgttttc ttaagatgtg
     3121 agatagaagg aaatactata aagattatag ttgaagatga agggtatgga atagaaaatg
     3181 tggagaaggc aatggaacca ctttatacat caaaaccaga acttgataga tcaggaatgg
     3241 gattcacagt aatgaaaagc tttatggacg atgtagaggt aagttctgta aaagataatg
     3301 ggactagaat tgaaatgact aaaaaaataa atgtgcctaa ataaagtcca aaagtgaggt
     3361 tttattatgg aagtaactgt tgccagagaa gaaaaaaaac ctcttcttag tcatgaagag
     3421 actctagaac ttattgaaaa agttcaaaat ggagatgaag aggcaaaaga aatattaata
     3481 tcaagcaatt taggtctagt taggagcgtt gtatctagat ttttaaatat aggatatgat
     3541 agagaagatt tatttcagtt aggttctata ggcttaataa aatcaatata taaatttgac
     3601 ccaaaattta atgtaaaatt ctctacttat gcagtaccaa tgattttagg tgaaataaag
     3661 agatacttaa gagatgatgg tatgataaaa gtatcaaggt cgttaaagca aatagctgta
     3721 aaggcaaaga tggaaagtga agctttaact aaaaaattag gtagagagcc aagtatagaa
     3781 gaattagcta aagcaataga tgtagaaaaa gaagatttag taatggctat ggaagctaat
     3841 ttcaatgttg agtatttgca aggagttatt catgaagagg aaggttctcc aatatgcctt
     3901 attgataaaa taagcatgaa aggagaaagt gaagaagaaa aagtagtgga taacatattg
     3961 ttaaaagaca tacttggaag actggataag agagagcgtc aaataatagt tctcagatac
     4021 tttgaagaca tgactcaaag tgaaatagga gagatgctca atatatcaca agttcaagta
     4081 tctaggatag agaagaaagt attgtctaag ttaaaagaat atatatcgtg ataatactta
     4141 tgatatgtag aataacaaaa tataataaat atattactaa aataaaatgt aataactttt
     4201 tatgttaagt ttttaaatta ttaaaagata ctaaaataat aaaaagcatt aaaaaagtaa
     4261 aaatataaaa acaaggtgtc tttatggaaa aatagatttt ttctttatta ggcatctttt
     4321 ttattttttg accaaacttt agatagtcaa aataaataat aggtttattt ttttaaaatt
     4381 aacaaataat actttagaga ggtgtttagt atggataaaa attataaaaa atatgtagac
     4441 caaataagcc caaaacctac atatttaaaa aactatacac ttgcatttat tgtaggtgga
     4501 attatttgta tgatagggca agcaataaat gatttgtata tgaaagttgg aggtcttgat
     4561 aaactaggtg ctagttctgc aacatcaata acattaatat ttataggggc atttttaact
     4621 ggattaggag tttacgacct aataggaaaa agagctggag ctggttctat aattccaata
     4681 acaggttttg ctaactcaat agtatctcca gcaatggagt ataaaagaga aggctatgta
     4741 ttaggtgtag gtgcaaactt atttaaaatt gcaggaccag ttttagttta tggtataggt
     4801 tcttctattt tatgtggaat tatttattat atttttaaga tgttttagat gatttttatt
     4861 taatttaaat tttattaatt atgaagaagg tataaaatct gaaaatagct actaaagtaa
     4921 ctaattgaaa aataattgtg cagatttaat taatattaaa aatttgaata gtaaaatgca
     4981 aaattaaaaa ggtgtgaaaa tatgaaaaat aaaagaattg gaaaaagaac agtcaagtta
     5041 gaaaataaac caaccataat atcaactgga actatagtgg gtcctaagga aggtgaagga
     5101 ccactaaagg actattttga tatgattatg actgacgatt tatatggtga aaagacttgg
     5161 gaattagctg aaagtaaaat ggtagagact gcatcccaac aagctatcca aaaggcaggt
     5221 aaaaaattgt cagatgtaaa ttatatgcta ggtggcgatt taataaatca aatagtgcca
     5281 gcatcatttg cagcaagaga actggctatt ccatttttag gtatatatgg agcttgttct
     5341 acaatggcag aaggtctttg tataggttct atgcttatag atggtggttt tgctgattta
     5401 gtactgtcag gtacatcaag tcactattgt acagcagaaa gacaatttag attcccactt
     5461 gaacttggaa atcaaaaacc aatgacagct cagtggacag ttacaggagc aggaagtgta
     5521 ttattagctc ctaatggaga cggtccaaaa gtaaagtatg ttactgttgg taaagtaata
     5581 gatgagggta ttgatgatgg taataatatg ggaccagcta tggctccagc agcaatagat
     5641 actatctact cttactttga agatactaat gatgacccaa atagctttga tataattgct
     5701 actggagatt taggtactct tggaaaacag atagcagaag actttctaaa agaaaaaggt
     5761 gtagatatat caaaagttta tactgactgt ggtatagaga tgtttaacct taaagaacaa
     5821 gatgtccact gtggtggaag tggatgtggg tgttcagcta cagtgtttgc aggatatata
     5881 tatgataagt tgagaaaaaa agaatttaac aaagtaatgc ttgtatcaac aggagcacta
     5941 ctttcaccga caagtacact acaaaagcag acaataccat gtgttgcaca tgcagtagta
     6001 atagtaaatg agtaggaagt gatataatat gttaatggac tatgtaagag tatttattgt
     6061 aggtggaatt atatgtttaa tagcccaagt aatgatggat tatttcaaaa tgcaaactcc
     6121 atatgttatg gtaacgtatg taacaactgg tgtagtttta acttttctag gactttatga
     6181 gccattagtt aagtttgcag cagcaggtgc aacagttcct ataataggat ttggatattc
     6241 acttgctaca ggagtaataa aatcaattca aagtgatggt tttttaggta tttttttagg
     6301 aggtacaact gctacagctg gtggaatagc agcagctata ttctttggat atattatgtc
     6361 agtagtattt actccaaaag caaaaccata aatctttatt aaaagaataa taagtttttg
     6421 aaataataac tttttataaa gcaggtggat atcttttaga ttccacctat tttttatata
     6481 aagaagcgtt tacataattg aagttgacaa ttatttacaa catagtgtag tattatatta
     6541 tcaaaataaa atgaagtgaa gtaggggata aagatggtaa ctattaaaga tattgcaagg
     6601 aatctaggta tcagttattc aactgtttct aggtgcttaa acaataatcc taatgtatct
     6661 gaaaaaacaa aaaagaaagt agtagaagag gcaaatagac tagggttcca ttttaatgta
     6721 aatgcaagaa atttagcaaa aaaagaaact aacaggatag gggtaatctt ttcaaataat
     6781 tttaatcatc aagatactag aaagtttttt agtgatataa tggatagttc tataaattct
     6841 attgagacta ataaatacga ttttataata cagcctaata ataacattag tggtgatagt
     6901 aatgtatata aaatggtaaa tggacaaatg gttgatggat tagtaattgt gtctcaatca
     6961 ataaaaaaag aagagtatga ttttttaaaa gacaataatt ttccacatgt atttatatat
     7021 tttaaaccat cttttgtagg agaagtagac aatttctttt gggacgataa cgtgtatgga
     7081 ggttatatag ctaccaagca tttaattgac catggtcata aagacataat aacagtaact
     7141 tcagatgata ggagttcaaa aatgcatgaa gatagaacta agggatattt aaatgctatg
     7201 gaagaggcaa atttaaagac cgaagtcatt aaatctaaaa tggattttga atctcaagtt
     7261 gaatttttag aaaaatatat tgataaaata aaaaaagctt cagctatatt tgtacaacaa
     7321 gatgtacctg cgatttctat aatacaagag ttaaaaacta cttatggtat aaatgtacca
     7381 gaagatattt ccataatagg gtataataat attgaattaa tatcatattt taggtcgcat
     7441 ttatcaacaa tagatgaccc aagagagcaa gttataaaaa atggagtaga tagtttagta
     7501 aatataataa ataaaaagag tacagaacac attccaagaa aattatatcc aaggttaata
     7561 atacgaaata gcgttaaaag aataaagtag taagctttta agtatcaaaa gtttattaaa
     7621 aatattatag attatgtgaa gttttttaac aaacaatata aataaaaatt aaaaggagta
     7681 ggtgtatata agtacattgt actcctttat ttattgagat agtttttata atatgaatga
     7741 ataattttat tcaattattt gaaaatccgt tattttaaca atataaatta ataaattatt
     7801 tgtattttat atcat
//
LOCUS       DAMJWP010000167         7807 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.173, whole genome shotgun sequence.
ACCESSION   DAMJWP010000167 DAMJWP010000000
VERSION     DAMJWP010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7807
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.173"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            351..1544
                     /gene="nifV"
                     /locus_tag="PC700_002818"
     CDS             351..1544
                     /gene="nifV"
                     /locus_tag="PC700_002818"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HDF2582097.1"
                     /translation="MCVISKDRAKEIKIVDTTLRDGEQTAGVVFANREKIMIAEMLSD
                     LGVDQIEVGIPTMGGDEKNVIKHICSRNLKSDIMAWNRAVIKDVEESISCGVDAVAIS
                     ISVSDIHIENKLRTSRGWVLENMAKTVEFAKKNGLYVSVNGEDASRADIDFLTEFINV
                     GKQAGADRFRYCDTVGVMNPFSIKNAIETLYERTNFDIEMHTHNDFGMATANALAGIA
                     AGANYVGVTVNGLGERAGNAALEEVLMALKCVYKCDLNNIDTRKFRGICEYVAQASGR
                     ILPTWKPVVGDNMFIHESGIHADGALKDPHNYEPFDPSEVNLERKIVIGKHSGRAAVI
                     NKLSEYEMYISPENATKLLNAIRATSIRLKRSLMDKEILQLYCDILAHEKGTTEEEAV
                     RGSYI"
     gene            1569..3494
                     /locus_tag="PC700_002819"
     CDS             1569..3494
                     /locus_tag="PC700_002819"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HDF2582098.1"
                     /translation="MGDNIVYKIIKKHIVDGEAVAGSSIGIKIDQTLTQDSTGTMTYL
                     QLEAMGIDKVKTKRSVAFVDHNMLQQGFENADDHKYIQTVADKYGVYFSKPGNGICHQ
                     VFLERFSTPGDTLLGSDSHTPTAGGVGMMAIGAGGLDVALAMAGGAYYIKAPKVCKVN
                     LVGKLNNMVSSKDIILEVLRKQTVKGGVGKVYEYGGEGVKSLSVPQRATITNMGAELG
                     ATTSIFPSDEKTLEFFKSQGREDAWIELKPDADAVYDEEITINLDELKPLAAKPHSPD
                     NVDEVENIGKIKIDQVAIGSCTNSSYEDLMKVAQILKGNKVHKDVSLVIAPGSRQVME
                     MIARNGALADIISAGARILENSCGPCIGMGQSPGTDSVSLRTFNRNFYGRSGTLSAQV
                     YLVSPEVAAVSAIKGVLTDPREFDIKFTNLDVNEFLIDDSMIIKPADVGSNVEVVRGP
                     NIKPFPLNTELSQSIGGKVILKTEDNITTDHIMPSNAKLLPFRSNIPYLANYCFNTVD
                     TEFPQRAKDNNGGFIVGGDNYGQGSSREHAALAPLYLGVKGVIVKSFARIHKANLINS
                     GIIPMEFCDEKDYENISLLDNLEIPNILDNLGSGILEVKNTTKGTSFKVKVELSAKEV
                     DVLKAGGKLNYTKNQAN"
     gene            3516..4511
                     /locus_tag="PC700_002820"
     CDS             3516..4511
                     /locus_tag="PC700_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HDF2582099.1"
                     /translation="MYKVTLIPGDGIGPEVAKAMKKVVEATGAEIEWEEVNAGEAVIE
                     EYGTPLPEYIIDSIKKNKIAIKGPITTPVGKGFRSVNVTLRQALDLYVNLRPIKSFKG
                     IKSRYEDVDLVVVRENTEDLYAGIEHKIGDYAAESIKIITRSASERIVDFACNYVKDN
                     KRKKVTAIHKANIMKMSDGLFLDVFREVASKHGVEYDDLIVDAAAMNLVLNPENYDVM
                     VMPNLYGDILSDLGAGLVGGLGIIPSANIGKDCAIFEAVHGSAPQIAGQNKANPTALI
                     QSSVMMLRYLGEYENAQKIETALEKVFLEGSKLTVDLGGSASTTEFADEVCKYIV"
     gene            4838..5311
                     /locus_tag="PC700_002821"
     CDS             4838..5311
                     /locus_tag="PC700_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2582100.1"
                     /translation="MVGSVEVCLIKTLDSNILREVGTLSRAVNSINDIKYKELKLQKG
                     QFTFLTRICENPGINLVELSNMLKVDKATTTKAIQKLIKAGYVDKKQDEFDKRGYNLT
                     PTDKSLEVYELIIEEENRSIEICFDNFTDEEKQVVTKLLEKMSKNVENEWFKVKR"
     gene            5345..5839
                     /locus_tag="PC700_002822"
     CDS             5345..5839
                     /locus_tag="PC700_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2582101.1"
                     /translation="MIRKANMNDLESIMKIIKSTVEEMKTYNNTQWDENYPLEKDFVS
                     DIKKQDLYIYEVDGEVAGFICLNYEEPEEYLGLNWSSNKKAMVIHRMAVNPNFRKTGI
                     ASKLVDFAEKLAVENNVSYLKSDTYSINSKMNLLLTKCGFIKIGEMSFLGKEKSFYCY
                     DKIL"
     gene            5936..6457
                     /locus_tag="PC700_002823"
     CDS             5936..6457
                     /locus_tag="PC700_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2582102.1"
                     /translation="MIGFLKKRRSIRKYKDVEVEKEKLDKILKAALLAPSSKGLRTWE
                     FIVVDDKEKLINLSQCRTKGGGFFLKNAPLAIVIIADKEKNDVWIEDASIAASYIQLQ
                     AHELGLGSCWIQVRNRMYDDNIEADKYIREELKVPSKYSVECIISIGYSDEEKKAYND
                     SDLDYKKVHFNNF"
     gene            6646..6957
                     /locus_tag="PC700_002824"
     CDS             6646..6957
                     /locus_tag="PC700_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF2582103.1"
                     /translation="MGIFKTKMDEDWKVNYIKEFNEMRDSYESKLQKKQFEVDSLKSE
                     LDRLRSYKNSLKPKEKQITDDDINNIKSLRRDGLSYKEISNQTSWSKATVSRVLNGLY
                     D"
     gene            6985..7671
                     /locus_tag="PC700_002825"
     CDS             6985..7671
                     /locus_tag="PC700_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2582104.1"
                     /translation="MKYSYKKCIIDSILLMFIIQILRMILNYVLLSQFEFTLENFNII
                     NLISFTLVGLSLILFLKDNSLYNKVRNRKITEAFEENKNNILIEKCKLILFVVVLSLA
                     IIVTYCTKGYVLFNVTMMTLSVLIVPIFEELFFREYIWNYLSNFIKSKSKIICITSIL
                     SGIYNIGYIDVIRNYVILYNNSSYTFEVIISKIMIGTVFGIVLGLVKYRFRDVGFCIL
                     LRSLFAIFIR"
BASE COUNT         3137 a          767 c         1497 g         2406 t
ORIGIN      
        1 tattaagata ctttaaaatg gaatgtaact taacttaaat tttagtagga attttatagg
       61 tttattttta aaggtttttt gaaaataaat ctatttaaaa attaaattaa aatgcaagaa
      121 aaacataata actttcattt ttaagaagtt ttcacaaaaa agaaaaaata tagtgcaata
      181 aatgatacaa agtaatataa tagattacaa gatatgaaaa gctaaaaaaa taaattgcaa
      241 atgaaattaa aatgaataaa aaataaaaaa ataatcataa gttagaggta tcaatgaaaa
      301 ataagactta atgtttttaa aattaaaagt atactaaggg gggcattaat atgtgcgtta
      361 tatcaaaaga tagagcaaag gaaataaaaa tagtagatac tactttaaga gatggagagc
      421 aaacagctgg ggttgtattt gcaaatagag aaaaaataat gattgctgaa atgttaagtg
      481 acctaggtgt tgaccaaatt gaagttggta tacctactat gggtggagat gaaaaaaatg
      541 taataaagca catatgtagt agaaatttaa aatcagatat aatggcttgg aatagagcag
      601 taataaaaga tgttgaagaa tctatttctt gtggtgttga tgcagtagct atatcaatat
      661 cagtatctga cattcatata gaaaataaat tgagaacttc tagaggctgg gttttagaaa
      721 atatggctaa aacagttgaa tttgcaaaga aaaatggatt atatgtttca gtaaatggag
      781 aagatgcttc aagagcagat atagacttct taactgaatt tataaatgtt ggaaaacaag
      841 caggggcaga tagatttaga tactgtgata cagtaggagt tatgaatcct ttttctataa
      901 agaatgcaat agaaactctt tatgaaagaa ctaattttga tatagaaatg catacacata
      961 atgactttgg gatggcaact gcaaatgcac ttgcaggaat agcagcagga gcaaattatg
     1021 ttggtgttac agtaaatgga ttaggtgaaa gagcaggaaa tgcagcatta gaagaagttt
     1081 taatggcact taagtgtgtt tataaatgtg atttaaataa tatagatact agaaaattta
     1141 gaggaatatg tgagtatgta gcacaggcat caggaagaat acttcctaca tggaaaccag
     1201 tagttgggga taatatgttt atacatgaat ctggtataca tgcagatgga gctttaaaag
     1261 acccgcataa ctatgaacca tttgacccaa gtgaagtaaa tcttgaaaga aaaatagtaa
     1321 taggtaaaca ttcaggaaga gctgcagtta taaataagtt gagtgaatat gaaatgtata
     1381 tttctccaga aaacgctacc aagctattaa atgctataag agctacatct ataagattaa
     1441 aaagaagttt aatggataaa gaaattcttc aattatattg tgatatacta gcacatgaga
     1501 aaggcacaac agaagaagaa gcagttagag gttcatatat ttaagatata attgtttagg
     1561 aggaaaatat gggagataat atagtttata aaataattaa aaaacatata gtagatggag
     1621 aagctgttgc tggaagttca attggtataa aaatagacca aactttaaca caagattcta
     1681 ctggaacaat gacatatctt caactagaag ctatgggaat agataaagta aagactaaaa
     1741 gaagtgtggc atttgtagac cataatatgt tacaacaagg atttgaaaat gcagacgacc
     1801 ataagtatat acaaacagtg gcagataaat atggagttta tttctctaag ccaggaaatg
     1861 gtatttgtca tcaagtattt ttagaaagat tttcaacacc aggagataca cttttaggtt
     1921 ctgatagcca cacacctact gctggaggtg tgggcatgat ggctattgga gctggtggat
     1981 tagatgttgc tttagccatg gcaggaggag catactatat aaaagcacca aaagtctgta
     2041 aggtgaatct agttggaaaa ttaaataata tggtatcatc aaaagatata atattagaag
     2101 tgttaagaaa acaaactgta aaaggtggag taggtaaagt ttatgaatat ggtggagaag
     2161 gtgtcaaaag cctatctgtt ccacaaagag ctacaataac aaatatgggg gctgaacttg
     2221 gagcaactac ttcaatcttc ccaagtgatg aaaaaacact agaatttttt aagagtcaag
     2281 gaagagaaga cgcatggata gagttaaaac cagatgcaga tgcagtttat gatgaagaga
     2341 ttactataaa cttggatgaa ttaaaaccat tagctgcaaa acctcatagc ccagacaatg
     2401 tagatgaagt tgaaaatata ggcaagatta aaatagacca agtggcaata ggaagttgta
     2461 caaattcttc ttatgaagac ttaatgaaag tagcacaaat cctgaaagga aataaagtac
     2521 ataaggatgt gagtttggta atagcaccag gttcaagaca agttatggag atgattgcta
     2581 gaaatggggc tttggcagat ataataagtg ctggagctag aatactagaa aattcttgtg
     2641 gtccatgtat tggtatgggt caatctccag gtactgactc tgtgtcttta agaactttca
     2701 atagaaattt ctatggtaga agtgggactt tgtcagcaca agtatattta gtaagtccag
     2761 aagtggcagc agtaagtgca atcaagggag tgcttacaga cccaagagaa tttgatatta
     2821 aatttacaaa tttagatgta aatgaatttt tgatagatga tagcatgata attaaacctg
     2881 ctgatgtagg aagtaatgta gaagtagtta gaggacctaa tataaaacct ttccctctta
     2941 atacagagct aagtcagtct ataggtggaa aagttatatt aaagacagaa gataatataa
     3001 ctactgacca tataatgcca tcaaatgcta agttacttcc ttttagaagt aatataccgt
     3061 atcttgcaaa ctattgtttc aatactgtag atacagaatt tcctcagaga gcaaaagaca
     3121 ataatggtgg atttatagtt ggtggagata attatggtca aggttcaagt agagaacatg
     3181 cagctttagc accattatat ctaggtgtta aaggggtaat agttaagagc tttgcaagaa
     3241 tccacaaggc taacttgata aatagtggta taattccaat ggagttttgt gatgagaaag
     3301 attatgaaaa tatatctctt ttagataatc tggagatacc aaatatttta gacaatctag
     3361 gtagtggaat tttagaagtt aagaatacaa ctaaaggaac ttcttttaag gtcaaagtag
     3421 aattgtcagc aaaagaagtt gacgtattaa aagctggtgg aaaattaaac tacactaaaa
     3481 atcaagctaa ttaatatgaa aagagggaat attgaatgta taaagtgaca cttataccag
     3541 gagatggaat aggaccagaa gttgcgaaag ctatgaaaaa ggtagtagaa gctactggag
     3601 cagaaataga atgggaagaa gttaatgcag gagaggcagt tattgaagag tatggtactc
     3661 cacttcctga atatattata gatagtataa aaaagaacaa aatagctata aaaggcccaa
     3721 ttactactcc tgtaggtaaa ggatttagga gtgtaaatgt aactttaaga caggcattag
     3781 atttgtatgt caatttaaga cctattaaaa gttttaaggg gataaaatca agatatgaag
     3841 atgttgattt agtggttgta agagaaaata cagaagattt atatgctgga atagaacata
     3901 aaataggtga ttatgcagct gaaagtataa agataataac aagaagtgca agtgaaagaa
     3961 tcgttgattt tgcatgtaac tatgttaaag ataataagag aaaaaaagtt acagctatac
     4021 ataaggcaaa tataatgaaa atgtctgatg gattgttttt agatgtgttt agagaagttg
     4081 catcaaaaca tggtgtagaa tatgatgatt tgattgtaga tgcagcagct atgaacttag
     4141 ttttaaatcc agaaaattat gatgtaatgg taatgccaaa tctttatgga gatatactat
     4201 cagatttagg tgctgggctt gttggaggtt taggtataat tccaagcgca aatattggta
     4261 aagattgtgc gatatttgaa gctgttcatg gaagtgctcc acaaatagca ggtcaaaata
     4321 aggctaatcc aacagcactt attcaatcat cagtaatgat gcttagatac ttaggtgaat
     4381 atgaaaatgc acaaaaaata gaaacagctt tagaaaaagt atttcttgaa ggaagtaaat
     4441 tgactgtaga tttaggtggc tcagcttcta ctacagagtt tgcggatgag gtgtgtaagt
     4501 atattgtata gattttattt agggaaaaag tgaaatatag attgaaggaa agaggctttc
     4561 tcaaaataga gataagcctt tttttattgg aatgataaaa tatatttgat tttatacaaa
     4621 agagagttcc tcataaacga aaaagttaat tttgggatat ccattttaat tttaaaatta
     4681 attgattaat acgcaaagaa agattaaaca aatttattat ttttaattga actttcaacc
     4741 aaaatatggt aaaatttaaa aaagttgaac tttcaactaa aaataaatat attgattatg
     4801 tgaaattcta taaaacagaa actatttgac tttagtcatg gtaggttcag tggaggtgtg
     4861 tttgattaag actttagata gtaatatatt aagagaagta ggtactttat ctagagcagt
     4921 aaattcaata aatgatatta agtacaagga attaaagtta caaaaaggac aatttacatt
     4981 tttgacaaga atctgtgaaa atcctggtat aaatcttgtg gaattatcaa atatgttaaa
     5041 ggtagacaag gctacaacaa ccaaggctat acaaaaacta ataaaggcag gatatgtaga
     5101 taaaaagcag gatgagtttg ataaaagagg atataattta actccaactg ataaatcttt
     5161 ggaagtttat gagttgataa ttgaagaaga gaatagaagt atagagatat gttttgataa
     5221 ttttacagat gaagaaaaac aagtggtaac taagttatta gaaaaaatga gcaaaaacgt
     5281 agaaaatgaa tggtttaaag taaagagata gtaaataaat acattaactt aaggagatat
     5341 ttttatgatt agaaaagcga atatgaatga tttagaaagc attatgaaaa ttataaaatc
     5401 tacagtggaa gaaatgaaga cttataataa tacacaatgg gatgaaaact atccattaga
     5461 aaaagacttt gtttcagata ttaagaaaca agatttatat atttatgaag tggatggaga
     5521 agtagcagga tttatatgtc ttaattatga agagccagaa gaatatttag gcttaaattg
     5581 gtcatctaat aaaaaagcaa tggtaataca tagaatggct gtaaatccaa actttagaaa
     5641 gacaggaata gcatctaagt tagttgattt tgctgaaaaa ttagctgtag aaaacaatgt
     5701 atcatactta aagagcgata catattcaat aaactcaaaa atgaatttat tacttacaaa
     5761 gtgtggtttt ataaaaatcg gagaaatgag ttttttagga aaggagaaat ctttctactg
     5821 ttatgataaa atattataaa gagcatttat aatacaaggg gtaaataata gattttcagc
     5881 attagtaagg gcataatata acccagtata tgctattatg aaagggatga tttatatgat
     5941 tggatttcta aagaaaagaa gaagtattag aaaatacaag gatgttgaag tagaaaaaga
     6001 aaaattagat aaaatattaa aagctgctct tttagctcca tcatcaaagg ggcttagaac
     6061 atgggaattt atagtggtag atgataaaga aaagctaatt aatttatctc aatgtagaac
     6121 taagggtggt ggatttttct taaaaaatgc acctttagct attgtaatta ttgcagataa
     6181 agaaaaaaat gatgtttgga tagaggatgc aagtatagct gcttcataca tacaacttca
     6241 agctcatgaa ctaggtttag gttcttgttg gatacaagta agaaatagaa tgtatgatga
     6301 taatatagaa gctgataagt acattagaga agaattaaaa gtacctagca agtacagtgt
     6361 agaatgtata atttctatag gatattctga tgaagaaaag aaagcataca atgactctga
     6421 tttagactat aaaaaggttc attttaacaa cttttagtat ataatatgtt gtattaagac
     6481 attatatttt tttctattgt taatatatac ttaaaattgt aaaaatatat ctgaatttta
     6541 ctaacttatc taggggttat gtacaaggat tttaaagata aatatagaaa ttaatgttta
     6601 taatcgatac ataacttgag taacaaaatt taaggaggtt aaagtatggg tatatttaaa
     6661 acaaaaatgg atgaggactg gaaagtaaac tacattaaag agtttaatga aatgagagat
     6721 tcttatgaga gtaaacttca aaaaaaacag tttgaggtag atagtttgaa atcagaattg
     6781 gatagactaa gaagctataa aaatagcctt aagccaaaag aaaaacaaat tactgatgat
     6841 gatataaata atataaagag tttaagaaga gatggcttaa gctataaaga aatttcaaat
     6901 caaacaagtt ggagcaaagc aactgtaagt agggtattaa atggacttta cgattagcat
     6961 atacatgggg tgatttaaat tgggatgaag tatagttaca aaaaatgcat aatagattct
     7021 attttattga tgtttataat tcaaatatta aggatgattt tgaattatgt acttctaagt
     7081 caatttgaat ttactcttga aaattttaat attataaact taatttcttt tacattagtt
     7141 ggtttatcgt taattttatt tttaaaagat aattctttgt ataacaaggt tagaaataga
     7201 aaaatcacag aagcttttga agaaaataaa aacaatatac ttattgaaaa gtgtaagctt
     7261 atactttttg ttgtggtttt atctttagca ataattgtaa cttattgtac taagggatat
     7321 gtacttttta atgtaactat gatgactttg tctgtattga tagttccaat atttgaagag
     7381 ttatttttta gagaatatat atggaactat ctaagtaatt ttataaagtc aaagagtaaa
     7441 attatctgta taacatctat actatctgga atttataata taggatacat agatgttata
     7501 agaaattatg taatattgta taacaattca agctacacct ttgaagttat tatttcaaaa
     7561 attatgatag gtactgtttt tggtattgta cttggattag ttaagtatag gtttagagat
     7621 gtagggtttt gtatattatt gaggagctta tttgctatat ttattaggta gatagtataa
     7681 tcggtttata aggttgtttt aaatttaatt ttaaatcaac cttattttat ttgtgtttta
     7741 taaaacactt atatattaat aactaataaa tttaataaag attatttgaa tatttttaaa
     7801 atatata
//
LOCUS       DAMJWP010000168         7798 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.174, whole genome shotgun sequence.
ACCESSION   DAMJWP010000168 DAMJWP010000000
VERSION     DAMJWP010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7798
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.174"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            108..1706
                     /locus_tag="PC700_002826"
     CDS             108..1706
                     /locus_tag="PC700_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF2582105.1"
                     /translation="MKAAIYSRKSRFTGKGDSIENQIQLCIDYAKSIGVKDFLIYEDE
                     GFSGSNIDRPQFKKMMQDAKDKKFSYLVCYRLDRISRNVSDFSTLIEKLNELNISFIS
                     IKEQFDTSTPMGRAMMFISSVFAQLERETIAERIKDNMYELARSGRWLGGNTPHGFNS
                     EKITFLDENLKERSMYKLQVNDEQMEIVKLIYNKYLELRSLSKLYKYVYNNGIRGPRG
                     GKFDPSSLSRILRNPAYVKANDEILDYLKNSGMDVVGTPDRKCGILTYAKNTSNSIAA
                     IAKHHGVIEPEIWLEVQNQLDKNRDKTPRISTGKTALLSGLIKCGKCGSNMRITYKGK
                     KTDKDLKYYYICGTKKTLGSDSCNCKNLNGPLVESLVIDKIKNCKEESIISAFNSNNI
                     KTDNVFNIFKSETNLLKNQILDKERLIKNLVIELAKNTGSTASDYIVSQIESLNNEIS
                     ILKDKINILESSNINISSDSINIDIIVSNLKRFNDEVDNASLDDKRLLLSTIINSIIW
                     DSSLGNLNIVYMGAEVDNSVSLSN"
     gene            1901..2701
                     /locus_tag="PC700_002827"
     CDS             1901..2701
                     /locus_tag="PC700_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2582106.1"
                     /translation="MRLNKRSEGYINYVKGVKREKRNVLFYQLLILIGFIVIWELLAD
                     LNVINTFLFSKPSDIYNLFIQYAFSGQLFKHIGISVYETVLGLVIGTVLGILVAIALW
                     WSEKLSKILDPFLVVLNALPKTALAPIIIVWVGAGIEGIVVTAVTISVVVTILSAYNY
                     FMNIDEEKIKMLKSFGATKSQILFKLILPANTGNLINLTKINIGMAWVGVIVGEFLVS
                     RYGIGYLIVYGSQVFKLDLVMMGVFVLAICAWAMYAVVNIIEKIYNSR"
     gene            complement(3612..4121)
                     /locus_tag="PC700_002828"
     CDS             complement(3612..4121)
                     /locus_tag="PC700_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2582107.1"
                     /translation="MLSENIKTIRKSKGLSQQELAIKLNVVRQTISKWEQGLSVPDSD
                     MLISISEVLETPVSTLLGETIVESEVDSLKVISEKLEIINLQLAQRKNMRRKMFHWLF
                     ILLCAVIVIVFVVLGGLNSPYLDWNYTDPETAVFGVAFHAFEWLFVRLVPIVLIGAII
                     GIFLTRKKI"
     gene            4943..5380
                     /locus_tag="PC700_002829"
     CDS             4943..5380
                     /locus_tag="PC700_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HDF2582108.1"
                     /translation="MNKKLENRCSWQEYFMRLCETVAERGTCDRAYVGAIIVNSENRI
                     VSTGYNGSISGDKHCSEVGHEMRDGHCIRTIHAEQNALYYCAKEGISVKDCSIYVTHF
                     PCLNCTKAIIQAGIKHIYYRTGYRIDEYAIKLLQSSNVLYTKL"
     gene            5519..6307
                     /locus_tag="PC700_002830"
     CDS             5519..6307
                     /locus_tag="PC700_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2582109.1"
                     /translation="MKVVIIPGHTLIGKGTGAVGYINESKETRILNDLIVKWLKIGGA
                     TVYTGRVDESSNHLADQCAIANKQETDLAVQIHFNSNATTSTPVGTETIYKTNNGKTY
                     AERVNTRLATVFKDRGAKSDVRGLYWLNHTIAPAILIEVCFVDSKADTDYYVNNKDKV
                     AKLIAEGILNKSISNSQGGGENKVYENVIVYTGDADKVAAQILHWQLKDSLIIEASSY
                     KQGLGKKVYVVGGEANKLVKGDVVINGADRYETVKLALQEIDKL"
     gene            6352..6810
                     /locus_tag="PC700_002831"
     CDS             6352..6810
                     /locus_tag="PC700_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582110.1"
                     /translation="MIRTKVVELIATVCRENKPHKWVDENYTPYDKSGKVELMSIEDL
                     NELISSSGRADFLYSSRLQKLLNEVYINQSRASYISGCGLFWSSYWDILEEKFEEWLY
                     NSYIFFDEDDEYLEGMEDFELECKDVLMDVIETTSIDIYVQMIKRNITNY"
     gene            complement(6904..7254)
                     /locus_tag="PC700_002832"
     CDS             complement(6904..7254)
                     /locus_tag="PC700_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582111.1"
                     /translation="MDNTITINISIIYVLLAILACIALVYLIIVLVKLHKILNNVGTM
                     LNENTQNINTTLNNLPNVVETFGEVGENMKDVTEVVTEVTADFLVTKDSVKSNMDVIV
                     DILTIIKNIFLDKK"
     gene            complement(7256..7639)
                     /locus_tag="PC700_002833"
     CDS             complement(7256..7639)
                     /locus_tag="PC700_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDF2582112.1"
                     /translation="MNLSKKIEDKRKAKQRAEKVKKAKIATAGVVLGAVTGAVSGVLL
                     APKSGKETREDIKDASQQIAEKINMKTVDVKGKVSEKLEDKKGNFIESKKKIKKYIDN
                     KKSVENSSEEDKIIDIVEPVSVTEE"
BASE COUNT         2855 a          970 c         1208 g         2765 t
ORIGIN      
        1 aattatataa atttacctaa ttaataaatt tattctaata agtttactta aaaggttgta
       61 tataaataat ttcaaatata tatcctaatt tataggaggt tttaattttg aaagctgcaa
      121 tttattcaag aaaatcaaga tttactggaa aaggtgattc tatagaaaat caaatacaat
      181 tatgtattga ttatgcaaag tctattggtg taaaagattt tttgatatat gaagatgagg
      241 gatttagtgg tagcaatata gatagaccac aatttaagaa aatgatgcag gatgctaaag
      301 ataagaaatt tagttaccta gtttgttata gattagatag gatatcgaga aatgtatctg
      361 acttctctac tttaatagaa aaattaaatg aactaaacat atcatttata tctatcaaag
      421 aacaatttga tacttcaact ccaatgggaa gagctatgat gtttatatca agtgtatttg
      481 ctcaattaga aagagaaaca atagcagaac gtataaaaga caacatgtat gaattagccc
      541 gtagtggtag atggcttgga ggcaatacac ctcatggatt caacagtgaa aaaatcacat
      601 tcttagatga aaatctaaaa gaaagaagta tgtataagct tcaagtaaat gatgaacaaa
      661 tggagattgt aaagctaata tacaataaat atttagaact taggtcctta tctaagttat
      721 ataaatatgt ctataataat ggaataaggg gtcctcgtgg tggtaaattt gaccctagtt
      781 ctttatctag aatacttaga aatccagcat atgtaaaagc taatgatgaa attttagatt
      841 accttaaaaa tagtggtatg gatgttgtag gtactccaga tagaaaatgt ggaatcctta
      901 cctatgctaa aaatacatct aactcaatag cagcaattgc aaaacaccat ggagtcatag
      961 aacctgaaat atggcttgaa gtacaaaatc aattagataa aaatcgtgat aaaactccaa
     1021 ggatatcaac tggaaaaact gctcttttat ctggtcttat aaaatgtggt aaatgtggtt
     1081 ctaatatgag aattacctat aaaggcaaaa aaactgacaa ggatttaaag tattattata
     1141 tttgtggtac taaaaaaaca cttgggtctg atagttgcaa ctgtaaaaat cttaatggtc
     1201 cattagttga aagtttagtt atagataaaa ttaagaattg taaagaagag tctattataa
     1261 gtgcattcaa tagtaataac attaagactg ataatgtttt taatattttt aaatctgaga
     1321 ctaatttact taaaaatcaa attttagata aggaaagact tattaaaaat ttagttatag
     1381 agcttgcaaa aaatactggt agtactgctt ctgattatat agtttcccag attgagagcc
     1441 taaataatga gattagtatt ttaaaggata agattaatat tttggaatct agtaatatca
     1501 atattagtag tgacagtatt aatattgata ttattgttag taatttgaag agatttaatg
     1561 atgaagttga taatgccagt ttagatgata agagattgtt attatctact attattaata
     1621 gtatcatttg ggatagcagt ttaggaaatc ttaatattgt ttatatgggt gctgaagtgg
     1681 ataattctgt ttctttaagc aattagttgc acttttttag ctatagcaga agcaataagc
     1741 atatcagata aggttgcaat actatctaaa agaccagcta ccataaaaaa tatttacgat
     1801 attgaactta acatggatgg aacaaaaacg cctctcaaaa cacgtaaagt tcctgatttt
     1861 caaaagtatt tcgacatatt atggaaggag ctagatacac atgaggctta ataaaagatc
     1921 tgaaggatat ataaactatg ttaaaggtgt aaaaagagaa aaaagaaatg tattattcta
     1981 ccagctatta attttaattg gttttattgt aatctgggaa cttttagctg acttaaatgt
     2041 tataaataca tttctattta gcaaaccaag tgatatttac aatttattta ttcaatatgc
     2101 atttagtggt caattattta aacatattgg aatatctgta tatgaaactg ttcttggtct
     2161 tgttataggt actgtattag gtattttggt agcaattgct ctttggtggt cagaaaaatt
     2221 atcaaaaata ttagacccat ttttggtagt tttaaatgct cttcctaaga ctgctttagc
     2281 tcctataatt atagtttggg taggtgctgg aatagaagga atcgtcgtaa cagctgtaac
     2341 gatttctgtt gttgttacta tactttcagc ttataattat tttatgaata tagatgaaga
     2401 aaaaattaag atgttaaaaa gctttggagc tactaaatct caaatattat ttaaactcat
     2461 attacctgca aacacaggaa atttaataaa tttaactaaa attaatattg gtatggcatg
     2521 ggtaggtgta atagttggag aatttttagt ttcaagatat ggaataggct acttgatagt
     2581 atatggttct caagtattta aattagattt agtaatgatg ggagtatttg tattggctat
     2641 ttgtgcttgg gcaatgtacg ctgtagttaa tattatagaa aaaatataca attccagata
     2701 aataaaaatt ttatttagaa tatttgatgt atcagtgatt ataataaaaa atgatttctt
     2761 tacagtaatc atttttttaa attaaaataa agcgtaagct tttgattatc ttcttataac
     2821 tcatcatgtt gctagcacag ctctatttta tgggctttgc tggcttttta ttgcctccaa
     2881 ttatatacat aatgcttaaa tgcagtcatt tctttattac ccatgcttaa atttataaaa
     2941 aagaaaagac agtcaacttt ttaatcaaaa ttaactgtgc tttatattta atatttaatt
     3001 ttcatttttg tgctccgttt gttagatagc agagacttaa ttttgtattt tgaacaggat
     3061 tgaatcttaa aaatttccca attatatcaa tttcaaagtc cattaagaat aatcctataa
     3121 gaactttgct aatttgatct aagaatatta aggatataac tggaaaaaca aaagacttta
     3181 tttgcctgat attaacacct ccttgcaaaa gtcattcact ttttccttac tcagtattgt
     3241 taaatctaaa aaaggaattt cgatttatta atttcacatc aataattata acatgagtaa
     3301 attatcaagt catttctttt ctgatttcgt agataatact tatctccata tatttctact
     3361 cgtagtcgtt gtacaaatcg ttgtaaatca tgctacagac ctcgcatttt ctgcatctga
     3421 cgcatgagag tataagtcca gcagtcttgc ttcctgtact ttacttaatg cggttttggg
     3481 cctactggcg tcctcaataa cttcgcttag ttggaaatca aaaaggttct tccttacata
     3541 gttgtcctcg attgccatgt agaatgacac ttttaaggaa cgcttgctat attataaaaa
     3601 gcagagttac ttcatatttt cttacgtgtc aaaaagattc caataattgc tcctataagg
     3661 acaatcggta ctaatctgac aaatagccac tcaaaagcat gaaaagctac tccaaaaaca
     3721 gcggtttcag ggtcggtata attccaatcc aagtaaggac tatttagtcc ccctaagacc
     3781 acaaaaacta ttactatgac tgcacataat aaaataaata accaatgaaa catttttctt
     3841 ctcatgtttt ttctttgtgc aagttgcaag tttataatct ctaatttttc agaaatcact
     3901 tttaagctat caacttccga ctcaacaata gtttctccaa gtaaagtgct tacaggcgtt
     3961 tcaagcacct ctgatatgga aatcaacata tcagaatcgg gaaccgacag tccttgttcc
     4021 catttagata ttgtttgtcg cactacattc agtttgatag caagttcttg ttgtgaaagt
     4081 ccttttgact tcctgattgt tttaatgttt tcgcttaaca ttagggctaa ccacctttct
     4141 ttcagttgtt accgatatta taggcaaaac tgcccgttgc ttcaagcaac gcattttaac
     4201 attcaagtaa atttcttctt tttcctttaa tcgttttgct tatatacagc ttttacgcgg
     4261 atattctcga tttttaagcg ctttttataa taaaaataga tatgtccagt aattaaaact
     4321 gtagaaatat ttataaaggc ataagacaaa tttacctaca atttttagtg tttagattct
     4381 aaaaaaataa aaataattat aattgagttc ataattttaa aacttattga atataattat
     4441 atgtcggtta aagacgtatg aaaataaaag gaatttacaa gttatgtgtt gtatagatat
     4501 tgtatatgca gcatgtaata aatcttagca ctatttatat tctatacaaa aagatatttg
     4561 aagggagatt aaaaatggga atattagaag gcgctgcaat gataagagag attgcaatta
     4621 aaatagctaa agaaaaaggt attacagaac aaaaggcatg gcccgaagct gtaaaagaat
     4681 ttaaagaaaa atatgaactt gtattataaa ctttgactgt aaaactgtct ctattttatt
     4741 gagacagttt tttatattgt aacattcata ttacgaagaa agatatatat aattttagat
     4801 tccttgtaac tttaatttaa aatgaaataa tttagactaa aaacagtatt aataaatttt
     4861 atttatcgta taaatttgtt tttaatatta tttttaaggt ataaatctta tatattagat
     4921 ataaattttg gaggtatatg atttgaataa aaaacttgaa aatagatgct catggcaaga
     4981 atattttatg agactgtgcg aaacagtagc tgaaagagga acttgtgata gagcttatgt
     5041 aggagctata attgtcaatt cagaaaacag aatagtgtct actggatata atggtagtat
     5101 ttctggagat aagcattgta gtgaagttgg tcatgaaatg agagatggac attgtattag
     5161 aaccattcac gctgaacaaa atgcccttta ttattgtgca aaagaaggta tttcagttaa
     5221 agattgctct atttatgtaa ctcactttcc ttgcttaaat tgtactaagg ctataattca
     5281 agctggaatt aaacatatat attatagaac tggatataga atagatgaat atgctataaa
     5341 attattgcaa tcaagtaatg tcttatatac aaaattataa ttttaacatt atttacttaa
     5401 tattctatat ttttacaaaa tgggttataa taaaagtata agatataaaa agaaacaaaa
     5461 ttctaggtta tgcatatatt aaatagaagt taaatttaat tgtaaaggag atgttagttt
     5521 gaaagtagta ataataccag ggcacacttt aattggaaaa ggtacaggag ctgttgggta
     5581 tataaacgaa agcaaagaaa caagaatact gaatgactta attgttaaat ggcttaaaat
     5641 aggtggagct actgtttata caggaagagt tgatgaatca agtaatcatt tagcagacca
     5701 atgtgctata gcaaataaac aggaaactga tttggctgtt cagattcatt ttaattcaaa
     5761 tgctacaact tcaactccag tagggacaga aacaatatat aagactaata atggcaaaac
     5821 ctatgcagaa agagtgaata ctaggttagc tacagtattc aaagataggg gtgcaaagtc
     5881 tgatgttaga ggtctttatt ggcttaatca tactattgca ccagcaatat taatagaagt
     5941 atgttttgta gatagtaaag cagatacaga ttattatgtt aataataaag ataaagttgc
     6001 taaattgata gcagaaggta tactaaacaa atctatatca aattctcaag ggggagggga
     6061 aaataaagtg tatgaaaatg taatcgttta tacaggtgat gctgataaag tggctgctca
     6121 gattttacat tggcaattga aagattcttt aataattgag gcgtctagtt ataagcaagg
     6181 tttaggtaaa aaggtttatg ttgtaggtgg agaagctaat aaacttgtaa aaggtgatgt
     6241 agtaataaat ggtgctgata gatatgaaac tgttaaatta gcattacaag aaatagataa
     6301 attataaaat catgattatt ttataaaaaa taaataagtt aggataaaaa aatgataaga
     6361 acaaaagttg tagagttgat agcaacagta tgcagagaaa acaaaccaca taaatgggtt
     6421 gatgaaaatt atactcctta tgataaaagt ggtaaagttg aattaatgtc aatagaagat
     6481 ttaaatgaat taatatcaag cagtggaaga gcagattttc tttatagcag tagattacaa
     6541 aagcttctaa atgaggttta tataaatcaa agtagagcat cttatatatc aggttgtgga
     6601 ttattttgga gttcgtattg ggatatactt gaagaaaaat ttgaagaatg gctatataat
     6661 tcttatatct tttttgatga agatgatgaa tatttagaag gaatggaaga ttttgagctt
     6721 gaatgtaaag atgttcttat ggatgtaatt gaaacaacaa gcatagatat atatgttcaa
     6781 atgataaaga ggaatattac caattattaa aaacacatag tgtcaaatca agctaataaa
     6841 aaacgacctt ataaatgtta ttttaaggtc gtttaaatta tatcttattt tatattaaat
     6901 ttattacttt ttatctagaa aaatattttt aattatagtc aatatatcga ctataacatc
     6961 catatttgat ttgacactgt ctttagtaac aagaaaatct gctgtaactt cagtaactac
     7021 ttctgttaca tctttcatat tttctcctac ttctccaaaa gtttctacaa cattaggaag
     7081 attatttaaa gttgtattta tattttgagt attttcattt aacatagttc caacattatt
     7141 taaaatctta tgtagtttaa caagtacaat aattaaataa actaaagcaa tacaagcaag
     7201 tatagctaat aatacatata ttatacttat gttaatcgtt attgtattgt ccatattatt
     7261 cctcagtaac actaactggt tcaactatat ctattatttt atcttcctca ctagaatttt
     7321 caacactctt tttgttatct atatattttt ttattttttt ctttgattct ataaagtttc
     7381 cttttttatc ctctagcttc tcagatactt tgccttttac atctacagtt ttcatattta
     7441 tcttttcagc aatttgttgt gaagcatctt ttatatcctc tcttgtttct tttccagatt
     7501 taggagccaa aagtactcca ctcactgcac cagtaacagc tcctaatacc acaccagctg
     7561 tagcaatttt agctttctta actttttcag ctctctgttt agcttttctt ttatcttcta
     7621 ttttcttcga tagattcatg tttaatcgct ccttattata atatttctaa acattataat
     7681 acattcttta ccataatgat atagtttcta actaaatttt taaaaatcaa taaaaattct
     7741 ttttaattac ttactattca ttacaattta aatttttata aggattttct atatattt
//
LOCUS       DAMJWP010000169         7753 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.175, whole genome shotgun sequence.
ACCESSION   DAMJWP010000169 DAMJWP010000000
VERSION     DAMJWP010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7753
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.175"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            89..364
                     /locus_tag="PC700_002834"
     CDS             89..364
                     /locus_tag="PC700_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2582113.1"
                     /translation="MKKILVACGAGIATSTIVCDRVERLVKENNIHAEVEQCKIAECS
                     TKQEGADLIVSTTILPTTYDIPTIKATGYITGINTTALDKKILNALK"
     gene            620..2320
                     /locus_tag="PC700_002835"
     CDS             620..2320
                     /locus_tag="PC700_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2582114.1"
                     /translation="MIKQFVKYYKPYKKIFTLDLIAAFLFSLCDLVYPMITRNIMDDV
                     VPNKNLRMLVVFAVALILIFIAKAGLNYFMQYWGHVIGVDMQADMRNEVFTHLQRLPN
                     TYFDNNKSGVTMSRIVNDLMDITELAHHGPEDLFISIVMLVGSFFILIDINIPLTLII
                     FAILPFIIWFAIAKKDKMNIAFMKSRVTIGDVNATLENSIAGMKVTKSFCTEKEELNK
                     FVRSNKLFRRARQDSYKVMAEYYSGMNLYMDILEWVVVIAGGYFTYIGKITLGDFAAY
                     ILYVKMFIQPMKKLINFTEQYQNGMTGFKRFIEIMEQDHQKEAKNPIELENVKGDIEI
                     ENISFTYEDKTQVLDNLSLSIKAGKTIALVGPSGGGKTTLCNLLPRFYEFDKGDIKID
                     GKSIKDVSLKSLRKNIGIVQQDVFLFTGTIRDNILCGNPNATDEEMIAAAKKARIHDF
                     VETLPDGYDTYIGERGVKLSGGQKQRISISRIFLKNPPIIILDEATSALDNVTEREIQ
                     ESLEELSKDRTNLVVAHRLTTIKNADEIIVLTDKGIEERGTHEELVNKNGVYSRLHNN
                     "
     gene            2683..3870
                     /locus_tag="PC700_002836"
     CDS             2683..3870
                     /locus_tag="PC700_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582115.1"
                     /translation="MEAITAFCLVLISLTIGDIVSAKTKAFVPSVFVSAIIFIVGFWT
                     FFPENIVDLAALGTPLAQLGMLLLITHMGTMMSIKELAGQWKTIVIALAGIVGICVGA
                     LALGTIVFGWDTAVIATPPLTGGLVASIMMADAATAKGLASLSVLAILMYVAQGFAGY
                     PITALMLKKEGKRLLSDFRSGKVTVNTDEEKVKDLPEQKSRFKIIPDLPEKYDTTYMI
                     ILRLGFVAWLAVQFTNLTNEVVSRYVICLIFGVIASELGIVDRKPLNKSGSFGWLMTS
                     LMAFIFAGLAKATPAMLVEMAVPLVGIIIFGVIGMGIFSIIVGKLLGESKEMAFSVSL
                     TSLYGFPPNYILTEEAVKALAETPEEKEFLMDKMLPKMLVGGFTTVTIASVVIAGIFV
                     NLI"
     gene            3930..5093
                     /locus_tag="PC700_002837"
     CDS             3930..5093
                     /locus_tag="PC700_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2582116.1"
                     /translation="MDIKEITKSYKDYVIKLRREFHENPEKSMEEVRTSKRVKEELDK
                     IEIPYVSAGGTGVIATIKGANPGKTVALRGDMDALQVVECTDVEYKSKNEGLMHACGH
                     DGHTSMLLGAAKVLNDIKDSINGTVKLFFQPGEEVGKGARAMIQDGAMEGVDSVFGIH
                     LWTDVESGTISVEEGPRMASADFFKITVKGRGGHGSLPHQGVDAVLASSAIVMNLQSM
                     VSREVSPLEPLVVSVGVLNSGTRFNVIASEAVLEGTIRLFNPELRKQIPGILERIAKS
                     TAEAYRADAELEYGYLTPAVINDKECSKIATDAAIKLFGEDCITLFEKVTGAEDLAEF
                     MNIAPGALAFVGARNESKGACYPHHHGCFNIDEDALEIGTALYVQYAVDFLNK"
     gene            5244..6047
                     /gene="zupT"
                     /locus_tag="PC700_002838"
     CDS             5244..6047
                     /gene="zupT"
                     /locus_tag="PC700_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HDF2582117.1"
                     /translation="MNNVIFAFLITLLAGLSTGIGSCIAFFAKKTNTKFLSISLGFSA
                     GVMIYVSMIEIFPKAQDALTKSMGEKLGSWSTVIAFFIGMAIIAVIDKLIPQEENPHE
                     IKKMENIEEKNIKKNKSLLRTGIFTAMAIAIHNFPEGLATFISALDDVTIAIPIAIAI
                     AIHNIPEGISVSVPVYYATGDKKKAFYYSFLSGMSEPLGAIIGYVLLRNFLNDITLGI
                     VFAIVGGIMVFISLDELLPSAREYGEHHLSIYGLIAGMGVMAISLLLFK"
     gene            6143..6703
                     /locus_tag="PC700_002839"
     CDS             6143..6703
                     /locus_tag="PC700_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HDF2582118.1"
                     /translation="MSAMEKKIKFKFDWYSSPDKIEKWLEDIEAKGYNLYKINKFGGI
                     FYFHKGSPRKIKYTVDYYYDVENNYFDEHKSNGWNLVFRSIGSFMGTSAWYIWSKEYK
                     DIPPKLYSDSDKKNKVKSILFMNMFIFFMLVVIFIYLLNIEIRNLYSGYGASVTAIVL
                     QIILLILYAVQLYRRLMYYLRLKKFK"
     gene            6954..7667
                     /gene="rgbR"
                     /locus_tag="PC700_002840"
     CDS             6954..7667
                     /gene="rgbR"
                     /locus_tag="PC700_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgbR"
                     /protein_id="HDF2582119.1"
                     /translation="MIKIAVCEDEKETQLLIEDYLENILKDISIEYEIQKYISGEELL
                     ESNLKDIDILLLDIKMEKLNGMDTARKIREVDDEMEIIFVTSLIDYVQEGYEVRAYRY
                     LLKPIELEELKKHVLTCIKDIEINKESHITIKNKSNTYKIYLNEIKYIEVQKKDMLIH
                     TINKNFDIKYSLGKIEKELNPYKFIRCHKSFIVNLRYVENIKPNAAILESGEEVPISR
                     YRYKEVKEKFLKFLGDTIC"
BASE COUNT         2970 a          795 c         1407 g         2581 t
ORIGIN      
        1 ataattattt atataaataa agatataaga aatttatcaa ttttattttg agttataaaa
       61 tattaaagaa attgtaggag gacaatatat gaaaaaaata ttagtagcat gtggagcagg
      121 aatcgcaaca tcaacaatag tttgtgacag agtagaaaga ttggttaaag aaaataatat
      181 acacgcagaa gttgagcaat gtaaaattgc tgaatgttca acaaaacaag aaggtgctga
      241 tttaatcgta tctactacta tactaccaac aacttatgat atacctacaa taaaggcaac
      301 aggatatata acaggtataa atacaacagc attagataaa aagatattaa atgcattgaa
      361 gtagttttgt gtttataaaa aaaatttagt attaaatata gagttgtatc tttataaaat
      421 tttaaatata gagatatagc tctttttatg ctgtttcaag tatacaacta tatgatataa
      481 atcttcaatt ttatgaaaaa gattgttatt actttgtaag tttataaaag atttaaagtt
      541 attgttatgt tatactaatt tatgggtatt ttttagaagt actatataga agtactaata
      601 aaggagggat ttaatactta tgataaaaca atttgtcaaa tattataagc catataagaa
      661 aatttttaca ctagacctta tagcagcttt tctattttcc ttatgtgatt tagtttatcc
      721 tatgataact agaaatataa tggatgacgt tgttccaaat aaaaacttaa gaatgttagt
      781 agtttttgca gtagcactta tacttatatt tatagcaaaa gcagggctta actattttat
      841 gcagtattgg ggacatgtta ttggtgtaga tatgcaagct gatatgagaa atgaggtatt
      901 tacacactta caaagacttc caaatacgta ttttgataat aacaaatctg gagtaactat
      961 gtctagaata gtaaatgatt taatggatat aacagaactt gcacatcatg gtcctgaaga
     1021 tttattcatt tctatagtaa tgttggttgg ttcattcttt atacttatag atataaatat
     1081 tcctcttacg cttataatat ttgctatatt accgtttata atttggtttg caatagctaa
     1141 aaaagataag atgaatattg cttttatgaa gagtagagtt actataggtg atgtaaatgc
     1201 tactttagaa aatagtatag caggtatgaa agtaactaag tctttttgta ctgagaaaga
     1261 agagttaaat aagtttgtaa gaagcaataa attatttaga agagcaagac aagattctta
     1321 taaggtaatg gcagaatatt actctggtat gaatttatac atggatatct tagagtgggt
     1381 agttgtaata gctggtggtt attttactta tataggaaaa attactttgg gtgattttgc
     1441 tgcttatata ctatatgtaa aaatgtttat acaacctatg aaaaaattaa taaattttac
     1501 agagcaatat caaaatggta tgacaggttt taaaagattc atagagataa tggaacaaga
     1561 ccatcaaaaa gaagctaaaa atcctattga acttgaaaat gtaaaaggtg acattgagat
     1621 agaaaatata tctttcacat atgaagataa gactcaagtt ttggataatt taagtttatc
     1681 tataaaagct ggtaagacta tagcattagt tggaccttct ggaggaggaa agactacatt
     1741 atgtaatcta ttgccaagat tttatgaatt tgataagggt gatataaaaa ttgatggaaa
     1801 gagtataaaa gatgttagtt taaaatcatt gagaaaaaat ataggtatag ttcaacaaga
     1861 tgtattttta tttacaggaa ctataagaga taatattctt tgtgggaacc ctaatgctac
     1921 agatgaagaa atgatagctg ctgctaaaaa agctagaata catgactttg tagagacttt
     1981 gccagatgga tatgatactt atataggaga aagaggcgta aaattatcag gtgggcaaaa
     2041 acaaagaatt tcaatttcaa gaatcttttt aaagaatcct ccaataataa tattggatga
     2101 ggctacatct gctttagaca atgtaacaga aagagaaatt caagagtctt tagaggaatt
     2161 aagtaaagat agaacaaatt tagttgttgc acatagattg actactataa aaaatgctga
     2221 tgaaatcata gtattgacgg ataaaggaat tgaagaaaga ggtactcatg aagagttagt
     2281 aaacaaaaat ggagtatata gtaggttaca caataactag tatacaaatt ttacaccata
     2341 gctaacatac gcattaaact tacaaacata ggatatatta agattcataa agacaattca
     2401 ttttatattt atgagatgtt ttatgaataa aatgtatcct atgttttatt ttttgcaaat
     2461 tacatttaat tctctttgta agaatagagt taaatagcca atggtcagga taacgttttt
     2521 acaaaaagaa taatttagaa aactaacttt aatttatgga atttatcgaa ttttatgaaa
     2581 aaaatcgaat tttgacttga aatgtttttc aaaagtagta aaataatgga taactataca
     2641 tacattttat aaaaatacat ttattatgag gggggatttt gtatggaggc aataacagct
     2701 ttttgtctag ttttaatatc actgacgata ggagatatag tttcagcaaa aactaaagct
     2761 tttgttccgt cggtttttgt atcagcaatt atatttatag taggattctg gacttttttc
     2821 ccagaaaata ttgtggactt agccgcttta ggaacaccat tagctcaatt aggtatgctt
     2881 ttgttaataa ctcatatggg aactatgatg agtattaagg aattagcagg tcaatggaaa
     2941 acaatagtaa tagccttggc aggaatagta ggaatatgtg taggtgcttt ggcattaggg
     3001 acaatagtat ttggatggga tacagcagta atagctacac ctcctcttac tggagggcta
     3061 gttgcatcta taatgatggc agatgctgct acagcaaaag gtcttgcaag tttatcagta
     3121 cttgcaattt tgatgtatgt tgcacaagga tttgcgggtt atcctattac agcattaatg
     3181 cttaaaaaag aaggaaaaag acttttatca gactttagaa gtggaaaagt aactgtaaat
     3241 acagatgaag aaaaagttaa ggatttacct gaacaaaaaa gcagatttaa gataattcca
     3301 gacttaccag aaaaatatga tacgacatat atgataatac ttagattagg atttgtagca
     3361 tggttagcag tacaatttac aaatcttaca aatgaagttg tatcaagata tgttatatgt
     3421 ttaatatttg gtgtaatagc atcagaactt ggaatagttg atagaaaacc acttaataaa
     3481 tctggttcat ttggatggtt aatgacatca ttgatggctt ttatatttgc aggattagca
     3541 aaagctacac cagcaatgct tgttgaaatg gcagttcctc ttgtaggaat cataatattt
     3601 ggtgtaatag gtatgggaat attctctatt atagtaggta agcttttagg tgaaagtaaa
     3661 gaaatggctt tttcagtttc gcttacatca ctttatggtt ttccaccaaa ctatatatta
     3721 actgaagagg cagtaaaggc tttggctgaa acaccagaag aaaaagaatt tttaatggat
     3781 aagatgcttc caaagatgct tgttggagga tttactacag ttacaatagc ttctgtagta
     3841 atagcaggta tctttgtaaa tctaatatag tatttaagtg ataaaaagta ttttaaataa
     3901 gaagaatgtg tactacttgg aggtatggaa tggatattaa agaaataaca aagagttata
     3961 aagactatgt aattaagctt agaagggaat ttcatgaaaa tcctgagaaa agtatggaag
     4021 aagttagaac atcaaagaga gttaaagaag aacttgataa aatagagata ccatatgtat
     4081 cagctggagg tacaggtgta attgcaacta taaaaggagc taatccagga aagactgtag
     4141 cacttagggg tgatatggat gctctacaag ttgttgaatg cactgatgta gaatacaaat
     4201 ctaaaaatga aggacttatg catgcttgtg gtcatgatgg tcacacatca atgcttctag
     4261 gggcagcaaa agtgttaaat gatattaagg atagtatcaa tggaactgta aaattatttt
     4321 ttcagccagg agaagaagtt ggtaaaggtg caagagctat gattcaagat ggtgctatgg
     4381 aaggtgtaga tagtgtgttt ggaatacatc tttggactga tgtagaatct gggactatat
     4441 cggtagaaga aggaccaaga atggcttctg ccgatttctt taaaattact gtaaaaggta
     4501 gaggtggtca tggttcactt cctcatcaag gtgttgatgc agtgcttgca agttcagcaa
     4561 tagttatgaa tctacaatct atggttagta gagaagtaag tcctctggaa ccattagttg
     4621 tgagtgttgg agttttaaat tctggaacta gatttaacgt aattgcgagt gaagctgtct
     4681 tagaaggtac aataaggcta tttaatccag agcttagaaa gcaaattcct ggaattttag
     4741 aacgtatagc aaaatctaca gcagaagcat atagagcaga tgctgaatta gagtatgggt
     4801 acttaacacc agcagttata aatgacaaag aatgttcaaa gatagctaca gatgcagcaa
     4861 ttaagctatt tggagaagac tgtataacac tatttgaaaa agtaacagga gcagaagatt
     4921 tagctgagtt tatgaatata gcacctggtg cactagcatt tgtaggagct agaaatgagt
     4981 caaaaggtgc ttgctatcct catcatcatg gatgctttaa tatagacgaa gatgctctag
     5041 aaattggaac tgctttatat gtgcaatatg cagtggattt cttaaataaa taattttaat
     5101 atactataaa gaaaaagcag agactaagtt tatatctctg cttttttatt gtgtatattt
     5161 tttataaata ttgactatat aaaatatata tgatagcata aagaaaatga taatcaatat
     5221 caattaaaaa gaaggtgatt tatttgaata atgtaatatt tgcatttttg ataacattat
     5281 tggctggttt atctactggt ataggaagtt gtatagcatt ttttgctaaa aaaacaaata
     5341 ctaagtttct ttctataagc ttaggatttt ctgctggtgt tatgatttat gtttctatga
     5401 tagaaatttt tcctaaagca caagatgctt tgacaaaatc tatgggagag aaattaggaa
     5461 gttggtcaac tgtaatagct ttttttatag gtatggctat cattgctgtt attgataagt
     5521 taattccaca agaagaaaat ccacatgaaa taaagaaaat ggagaatata gaagaaaaga
     5581 atatcaaaaa aaataagtct cttttgagaa caggtatatt tacagcaatg gctatagcaa
     5641 ttcacaattt tcctgaagga ttagctacat ttatatcagc tcttgatgat gttacaatag
     5701 ctatcccgat agcaattgca attgcaatac acaatatacc agaaggtata tctgtatctg
     5761 taccagttta ttatgccaca ggagataaaa agaaagcttt ttattattcc tttttatctg
     5821 gtatgtctga gcctttagga gcgattattg gatatgtact tcttagaaat tttttaaatg
     5881 atataactct tggaattgta tttgcaattg taggaggaat tatggtattc atatcactag
     5941 atgaattatt gccatcagct agagagtacg gggaacatca tttatctatt tatggattga
     6001 ttgcaggaat gggtgttatg gctattagtt tattactatt taaatagctt tttaaatata
     6061 tgattgtaac ctaaaattag tatcaaaatg atataattat ttaaatagat ttttaagcta
     6121 tttaaaaata tcattttgga gaatgagtgc tatggaaaag aaaataaaat tcaaatttga
     6181 ctggtatagt tcacctgata agatagagaa atggttagaa gatattgaag ctaaaggata
     6241 taatttatat aagataaata aatttggagg aatattttat tttcataagg gaagtccaag
     6301 aaaaataaaa tacactgttg attactacta tgatgtagag aataattatt ttgatgaaca
     6361 taaatcaaat ggctggaatc ttgtatttag gagtattggt agttttatgg gtacatctgc
     6421 atggtatata tggtcgaaag aatacaaaga catacctcct aaattatata gtgattctga
     6481 taaaaaaaac aaagtaaagt caattttatt tatgaatatg tttatattct ttatgttagt
     6541 agtaatattt atatatttat tgaatattga aataagaaat ttatatagtg gttatggagc
     6601 atctgtaact gctattgtac tacagattat attgttgatt ttatatgcag tacaattata
     6661 taggagatta atgtattatt taaggcttaa aaagtttaaa taaataaaag aaggtgtcct
     6721 aaaattattt ttttaatatt ttaagacatc ttcttttgtt agtaaagtac tgtttttaat
     6781 tatagtaatt tctgtcttta aattgaaata tcaaataata gttgtattgg attgagtatt
     6841 gataaaaata atttaaaaat gttatcctaa agtatcagat atacaaaatt atctttaaat
     6901 atattataaa tattttaatt gtattaacta gaaattatgt aaggaggcat tttatgatta
     6961 agatagcagt ttgtgaagat gaaaaagaaa cacaactttt aatagaagat taccttgaga
     7021 atatattaaa agatataagt atagaatatg agatacaaaa gtatatatct ggagaagagt
     7081 tactggaaag taatttaaaa gatatagata tattacttct tgatataaaa atggaaaaat
     7141 taaatggaat ggatactgca agaaagatta gagaagtaga cgatgaaatg gaaataatat
     7201 ttgtaacttc attaatagat tatgtgcaag aaggttatga ggtaagagct tataggtatt
     7261 tactaaagcc aatagagttg gaagaactaa aaaaacatgt gctaacttgt atcaaagata
     7321 ttgaaataaa taaggaaagt catattacaa taaaaaataa gtctaataca tataagattt
     7381 atttaaatga aataaaatat atagaagttc aaaaaaaaga tatgctaata cacacaataa
     7441 acaagaattt tgatataaaa tatagtttag gtaaaataga aaaagaatta aatccatata
     7501 aatttataag atgccataaa agttttatag taaatttaag atatgttgaa aatataaagc
     7561 ctaatgctgc aatactggaa agtggagaag aggtacctat tagtagatat agatataaag
     7621 aagttaagga gaaattttta aaatttcttg gtgatacaat atgctaaatt ttatgaatga
     7681 gctattagta gttttatcta tatttataca gttttctata atgaacggaa ttttaattta
     7741 taaaaaggat agt
//
LOCUS       DAMJWP010000170         7744 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.176, whole genome shotgun sequence.
ACCESSION   DAMJWP010000170 DAMJWP010000000
VERSION     DAMJWP010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7744
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.176"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            132..443
                     /locus_tag="PC700_002841"
     CDS             132..443
                     /locus_tag="PC700_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDF2582120.1"
                     /translation="MYFTYIIRCKDDSLYTGYTSNIVRRMNEHKLGINSKYTRAKGFE
                     KLEVYFVTNTKSNAMKLEYYIKKLTRNKKLSIIKNPSILINLIDNKEDYIIGKEIEQL
                     T"
     regulatory      536..628
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            728..1522
                     /gene="thiD"
                     /locus_tag="PC700_002842"
     CDS             728..1522
                     /gene="thiD"
                     /locus_tag="PC700_002842"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDF2582121.1"
                     /translation="MSNYKIPTLTIAGSDSSGGAGIQADLKTFSAIGTYGMSVITAIT
                     AQNTKGVFAVEDLNKKIIKKQIEAVFEDIPPRAVKIGMVSSPEIILEIVENLKKYNPK
                     YLVVDPVMISKSGYYLLKPEAKENLIKYLIPLAYIITPNIPEAEEITGIKIHNVDDMK
                     RVGEEILQLGPKFVLMKGGHLDGEAVDILVGKNIFKVYKSERIDKKNTHGTGCTLSSA
                     ITSYLALGYEITEAVNLSKIYITEAIKRSFDIGHGVGPVHHFYKFE"
     gene            1546..2334
                     /gene="thiM"
                     /locus_tag="PC700_002843"
     CDS             1546..2334
                     /gene="thiM"
                     /locus_tag="PC700_002843"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HDF2582122.1"
                     /translation="MYNLIKDVKKLNPLVIHYTNNVTINDCANVTLAVGASPLMSFSY
                     EEVEEMVSVANSVVINIGTMNSNMLDLFLLAGKAANKYNKPVVLDPVGVFASKARAEL
                     TSRLLNEVKFSVVKGNVSEIKFIGGFNVRGKGVDSFDEEEDSTEIIRKIAEKLECVVV
                     ATGKIDIITNGKGIYKINNGTDKLKGITGTGCMTASLIASFMAVTENILEAATMGVLT
                     MSLSGELANLNNPPIGTFKENLMNAIYQMDIDTLSKNSNIEFLN"
     gene            2357..2989
                     /gene="thiE"
                     /locus_tag="PC700_002844"
     CDS             2357..2989
                     /gene="thiE"
                     /locus_tag="PC700_002844"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDF2582123.1"
                     /translation="MIDKESLKKCLKLYLVTDSEMLKGRDFYKCLEDAISSGITTVQL
                     REKNASGREFLRKAMKLREITERYGVKFIINDRVDIALICDADGVHVGQSDIDVREVR
                     KLIGNNKILGVSARTLEEATCAKNDGADYLGVGSIFTTSTKLDAKSASFETVKEIKEK
                     VDMPFVLIGGINLDNIDKLKCLESDGYAIISAILKAEDISKEVEKWTLKI"
     gene            3128..3430
                     /locus_tag="PC700_002845"
     CDS             3128..3430
                     /locus_tag="PC700_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2582124.1"
                     /translation="MIKILLVCVGGMSSTLLVNKMEKDAKKRNIDCKIWAVGEGDIKS
                     ELDNFDILLLGPQLRFMLDDVKSIVGDRVPVSIIDMVNYGTCNGHAVLNSVLEILK"
     gene            complement(3496..4245)
                     /locus_tag="PC700_002846"
     CDS             complement(3496..4245)
                     /locus_tag="PC700_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2582125.1"
                     /translation="MLNILWRSMKWRFKNPISFVVTILQPFLWLVLYSSIANQTMNNI
                     NINNYTAFILPGIIVLVVFSSCSSGGIINFIMKNSGSFYRVLIAPISRYSIVLGQLLE
                     AILVSFIEVTILCIVSIFFSVRIESGIGGILLMIVLIFMTAFFLSSLAYSISLLLPNE
                     IVYETIMTAIVLPIFFLSSALFPIESLSGGLKVAVMLNPFTHVINALRSLIFGETILI
                     GDILPVILLFLILCCSSFSLAMWRLKKEMVS"
     gene            complement(4239..5201)
                     /locus_tag="PC700_002847"
     CDS             complement(4239..5201)
                     /locus_tag="PC700_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2582126.1"
                     /translation="MLAIEIDNLVKEYKNGVKALNGLSFNVNAGEIFSLLGPNGAGKS
                     SLINILTTFYKPTSGNVKMFGKDLVDNPSWIRTQIACVAQQISIDEHLSLMENMVFQS
                     KMYKVEPQIAKQRIDSLIDKFDLSSYLKYPTSSYSGGVKRRLDIAMNMVSSPKILFLD
                     EPTVGMDVDSRKSMWDMLLKIRDEYGTTIFLTTHYLEEAEQLSDNICIMKNGKDLAQG
                     TPSSLRSYIRQNILRITFHNTEDIKKYKDSIKSTGLVKFMSVRENSIFISVNDSRTAF
                     TLINKWLLEHDIEFDAIEIVEPSLEDVFLALTSSKKSLKEEWEC"
     gene            complement(5205..5663)
                     /locus_tag="PC700_002848"
     CDS             complement(5205..5663)
                     /locus_tag="PC700_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDF2582127.1"
                     /translation="MNCKIEIKNIESVTVATMRYNGPMTEATKYFPNVFKAIKGKSNG
                     APFFCYYDVDQKTGVAEMELCVPTAETPNRMGITTKEFPKTKALFFTHTGSYSNLPKT
                     YEMIFKYIHENNIKIQTPWREVFIKGPGMLIKGNPDNYITEIIFPLKEEE"
     gene            6019..6375
                     /locus_tag="PC700_002849"
     CDS             6019..6375
                     /locus_tag="PC700_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2582128.1"
                     /translation="MNFELDNTTPIYLQIVKYIKRQIVTGELKPGETIPSRREMALNL
                     KVNLNTVQRAYKEMGDMNIINTFKNYQSSVTVDENILKNLKLELINESLSVFIEDMKA
                     INVSKEEVLKIIEDKY"
     gene            6447..7163
                     /locus_tag="PC700_002850"
     CDS             6447..7163
                     /locus_tag="PC700_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2582129.1"
                     /translation="MIEIKNVSKTYKRMQGLKIRKIEALKNVSFNIEKGKITALLGIN
                     GVGKSTMLKAIAGLINIDSGEIRIDGEKINEKVYNKLAFVPDVQSHFSNTTIKETFEF
                     MEIFYSKWNKEKSKEMMDIFKLDEDEIIDNLSKGNIARVKLILGFCQDPEYILLDEPF
                     TGIDLFKREEFIGVIAQYMEENQAIIITTHEIVEIESLVDEVVILDEGQIITSFNAEE
                     LREREGKSILDKMREVYKNE"
     gene            7156..>7744
                     /locus_tag="PC700_002851"
     CDS             7156..>7744
                     /locus_tag="PC700_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2582130.1"
                     /translation="MNKLLYLINVDLRRSNKFYIAYISFFSLITLSLNLFEINKFKNS
                     KFIMNLAIEDFGGIFYGLGILSNLGFIQNILFFGMLGLFIYYIFMWKREFFSNNQSIY
                     TLMMLPQNKFKICISKSIVLITMIYGFLISQTATLFICKYIFNFIFRNIPIINMNFAK
                     DLYYLNLKSIPIDFISFMAIYVFLLLIAISIVNCCI"
BASE COUNT         3005 a          895 c         1269 g         2575 t
ORIGIN      
        1 gattcatgta gagtatgttt tatacatata ttataattaa ttctttgaag attgcatatt
       61 caaaatacac accatttttt ttaattctat aatataatat ttatatattt aatgttagga
      121 tgtgatttaa ggtgtatttt acatatatta taagatgtaa agatgattct ctatacactg
      181 gatataccag taatatagtt agaagaatga atgaacataa gttaggaatt aattcaaaat
      241 acacaagagc aaagggattt gaaaaactag aagtttattt tgtgaccaac acaaaaagca
      301 atgctatgaa gttagaatac tatataaaaa aattaactag aaataaaaaa ttgtctataa
      361 taaaaaaccc aagtatactt ataaatttaa ttgataataa agaagattat attattggta
      421 aggaaataga acaattaaca taaatattta tagtaacatt ataatgggtg catatagtgt
      481 aaaaacttga caataaatac tattgtaata taatgaagtt ataaatatag taaattaaaa
      541 tagctagggg agccaagagc tgagaggtat accgaccctt acacctgatc tggataatac
      601 cagcgtagga aagcttagta aaaattcaag tatgtattat ttgtgatata tgctatttct
      661 aagctatacc tattgtaggt atagcttttt taattaagtt atttaaatta aactttagga
      721 ggcaacaatg agtaattata aaataccaac acttacaata gctggctctg attcatctgg
      781 aggagctggg attcaggctg atttgaaaac gtttagtgca attggtactt atggtatgag
      841 tgtaataaca gctataacag cacagaatac taaaggagtt tttgctgtag aggatttaaa
      901 taagaaaata ataaaaaaac aaatagaggc agtctttgaa gatatacctc caagagctgt
      961 aaaaatagga atggtttcaa gtcctgagat tatacttgaa atagttgaga atctaaagaa
     1021 atacaatcca aaatatttgg ttgtggaccc agtaatgata tctaaaagtg gatattatct
     1081 attaaagcca gaggcaaaag aaaacttaat taagtatttg ataccacttg catacataat
     1141 tacaccaaat ataccagagg cagaagaaat aacaggaata aaaatacata atgtagatga
     1201 tatgaagaga gtaggagaag aaatactaca acttggacct aaatttgtac ttatgaaagg
     1261 aggtcattta gatggagaag cagtggatat tttagttggt aaaaatatat ttaaagttta
     1321 taaaagtgaa agaattgata aaaaaaatac acatggaaca gggtgcacat tatcttctgc
     1381 aataacatct tatctagcat taggatatga aataacagaa gcagtaaatc taagtaaaat
     1441 ctatataaca gaagcaataa aaagaagttt tgatataggt catggagtag gaccagtaca
     1501 tcatttttat aaatttgagt aataaaaaaa tattggaggt agaatatgta taatttaatt
     1561 aaagatgtaa aaaaattaaa tccattggta attcattata ctaataatgt aactataaat
     1621 gattgtgcta atgtgacttt agctgtagga gcaagtcctc ttatgagttt ttcatatgag
     1681 gaagtagaag aaatggtaag tgtagcaaat tctgtagtaa taaatatagg aactatgaac
     1741 tcaaatatgt tggatttatt tctattagct ggaaaagcag caaataagta caataaacct
     1801 gttgttttgg acccagttgg agtatttgca agcaaagcga gagcagaact tacaagtaga
     1861 cttttaaatg aagtcaaatt tagtgttgtt aaagggaatg tatcagaaat aaagtttata
     1921 ggtggcttta atgtaagggg taaaggtgta gattcatttg atgaagaaga agattctact
     1981 gagataataa gaaagattgc tgaaaagctt gaatgtgtag ttgtagcaac tggaaaaata
     2041 gatataataa ctaatggtaa aggtatatat aaaataaaca atggaactga taaattaaaa
     2101 ggaattacag gaactggatg tatgactgct agtttgattg caagttttat ggctgttact
     2161 gagaatatat tagaagcagc aacaatgggt gttcttacaa tgtctttaag tggggaatta
     2221 gctaatttaa ataatccacc aataggtaca tttaaagaaa atcttatgaa tgctatttat
     2281 caaatggata tagatacttt aagtaaaaat tctaatattg aatttttaaa ttaataaaga
     2341 gtaagggaga cttaaaatga ttgataaaga atctttaaaa aaatgcttaa agttgtattt
     2401 ggtaactgac tctgaaatgc ttaaaggtag agacttttat aaatgtcttg aagatgcaat
     2461 atcttctgga ataacaactg tacaacttag agaaaaaaat gcttctggaa gagagttttt
     2521 aagaaaagct atgaaactta gagaaataac tgaaagatat ggtgttaagt ttataataaa
     2581 tgatagagtg gatatagcac ttatttgtga tgcagatgga gtacatgtag gacagagtga
     2641 cattgatgtg agagaggtta gaaagcttat aggaaacaat aaaattctag gagtatcagc
     2701 aagaacttta gaagaagcta cttgtgcaaa aaatgatggc gcagactatt taggtgtagg
     2761 gtctatattc acaacatcta ctaaattaga tgcaaaatca gcttcgtttg aaactgttaa
     2821 agaaattaaa gaaaaagtag acatgccatt tgtgctaatt ggaggtataa atttagacaa
     2881 tatagataaa cttaaatgtt tagaaagtga cgggtatgct ataatatcag ctatattaaa
     2941 agctgaagat atctctaaag aagtggaaaa gtggacatta aaaatataaa attggctctg
     3001 gtagtgaaaa aaatggaaaa atttgatata atctaagagt aaaatttaag caattataaa
     3061 tgataaactt ttctacatat actttttaat caagatatat gtagatttgg ttaaaaagga
     3121 gctataaatg attaagatat tgttagtgtg tgttggaggc atgtcttcta ccctattggt
     3181 aaataaaatg gagaaggatg caaaaaaaag aaatatagac tgtaaaatat gggcagttgg
     3241 agaaggagat ataaaatcgg agcttgataa ctttgatatt ttacttcttg gaccacaact
     3301 tagatttatg cttgatgacg taaagtctat agttggagat agagttccag tatctataat
     3361 agatatggta aactatggta cttgtaatgg tcatgctgtg ttaaattcgg ttttagaaat
     3421 attaaaataa ataataaaag tggattctat ataatagagt ccatttttat tatttgttat
     3481 taataggctt aaaacttagc ttaccatttc tttttttaat ctccacatag caagtgaaaa
     3541 acttgaacag cacaatatta aaaatagtag gataacaggc agtatatctc caataagaat
     3601 agtctcacca aatattaagc tacgtaaagc gttaataaca tgggtaaatg gattgagcat
     3661 cactgctact tttaatcctc cagataaact ttctataggg aaaagagctg aacttaaaaa
     3721 gaaaatagga agtacaattg cagtcataat tgtttcataa actatttcat ttggcaataa
     3781 taaacttata gaataagcta gacttgataa aaagaaggct gtcataaata tcagtactat
     3841 cataagtagg attccaccaa tgccagattc tatccttact gagaagaata tacttactat
     3901 acacaaaata gttacttcta tgaaggaaac taagatagct tctaataatt gaccaaggac
     3961 gattgaatac ctactgatag gtgcaattaa gactctatag aaacttccac tgttcttcat
     4021 tataaagttt ataataccac cactactaca agaagaaaac acaaccagta caatgatacc
     4081 tggtagtata aaagcagtat aattattgat atttatatta ttcatagttt ggtttgcaat
     4141 agaactataa agcacaagcc aaagaaaagg ttgtaatata gtaactacaa atgagattgg
     4201 atttttaaaa cgccacttca tactacgcca taagatattt aacattccca ttcctccttt
     4261 aagctttttt tagaacttgt aagtgctaaa aatacatctt cgagacttgg ctctacaatt
     4321 tctattgcat caaattctat atcatgttct aaaagccatt tatttattaa agtaaaagca
     4381 gttctactat cgtttacact tataaaaatt gaattttctc gtacgctcat aaactttact
     4441 aaaccagtac ttttaattga atctttatat ttttttatat cttcagtatt atggaaagtt
     4501 atacgtagta tattttgtct aatgtaacta cgtaaacttg atggtgttcc ttgtgctaaa
     4561 tcctttccat tcttcataat acaaatatta tcactcaatt gttcagcttc ttcaagataa
     4621 tgagtagtta gaaatatagt tgtgccgtac tcatctctaa tttttaataa catatcccac
     4681 atagattttc ttgaatcgac atccatacca acagttggtt catctaaaaa taggatttta
     4741 ggagatgata ccatattcat agctatgtcc aatctacgtt ttacaccccc tgaatatgat
     4801 gaagtaggat attttaagta actagataag tcaaacttat caattaaaga gtctattctt
     4861 tgttttgcaa tctgtggttc tactttatac attttacttt gaaataccat attttccata
     4921 agggataaat gttcatcaat agagatttgc tgtgctacac aagcaatttg agtacgtatc
     4981 caagaaggat tatctactaa atcttttcca aacattttta catttccaga tgtaggtttg
     5041 taaaaggtag tcaaaatatt tataagagaa gatttaccag caccattagg accaagtaaa
     5101 gaaaaaattt ctccagcatt tacattaaag cttagaccat ttaaagcttt cacaccattt
     5161 ttatattctt ttactaaatt gtctatttca atcgctaaca tatattattc ctcctcttta
     5221 agtggaaata ttatctctgt aatatagtta tctgggtttc cttttatcaa catacctggt
     5281 cctttaataa atacttctct ccaaggagtt tgtattttta tattattttc atgaatatat
     5341 ttaaatatca tttcatacgt ttttggcaaa ttactataag agcctgtgtg agtaaaaaat
     5401 aaagcttttg ttttaggaaa ttcttttgta gttataccca tacgatttgg agtttcagca
     5461 gttggaacac ataattccat ttctgcaaca ccagtttttt ggtcaacatc atagtagcaa
     5521 aagaatggtg caccgttaga ctttcctttt atagctttaa atacatttgg aaaatacttt
     5581 gttgcctcag tcataggtcc attataacgc atggtagcca ctgtgactga ttcgatattt
     5641 ttgatttcaa ttttgcaatt catttgttta cctcctaatg tgtgttcata ttcttactag
     5701 atttttaagc attaatacat ataagatttt gattagttga aaacatataa tcttatctcg
     5761 tttgcttgtt ttcaagtcta acataacata aaatatatat cctctcattt gatgctccat
     5821 tttgtaaagc tattatttaa gctttacttt agatttttta gcaaattgaa ataaagcaat
     5881 ttataaaata agtatttaca aattaagtgt attaatcgta taatacactt aataggatac
     5941 tagataaagt ctgaaataaa ggcgtatttt tttaaagtaa gtgtactatc gtattaatac
     6001 actagaatag gaggaatcat gaattttgaa ttagataata caactcccat ttatttgcaa
     6061 atagtaaagt atataaagag acaaatagta acaggagagt tgaaaccagg agagacaata
     6121 ccatcaagaa gagaaatggc acttaatcta aaagtaaact taaatacagt tcaaagagca
     6181 tataaggaga tgggtgatat gaatataata aatactttta aaaattatca aagcagtgta
     6241 acagttgatg aaaatatatt aaaaaattta aaattagagc ttataaatga atcactatct
     6301 gtttttatag aagatatgaa agcaataaat gttagcaaag aagaagtttt aaaaataata
     6361 gaagataagt attagttttt taacaaatag aaaacttagt ttagaaagtt tatttataag
     6421 catattggat gtggaggata gtaaatatga tagaaataaa gaatgtcagc aaaacatata
     6481 aaaggatgca agggcttaaa ataagaaaga tagaagcttt aaaaaatgta agttttaata
     6541 tagaaaaggg aaaaataact gcattacttg gtataaatgg tgttggtaaa tcaactatgt
     6601 taaaagcaat agcaggactt ataaacattg atagtggaga aattcgtata gatggagaaa
     6661 aaataaatga aaaggtatac aataaattgg cttttgttcc agatgtacaa tctcacttta
     6721 gcaatacaac aataaaagaa acatttgaat ttatggaaat tttttattca aagtggaata
     6781 aagaaaaatc aaaagaaatg atggacatat ttaaattgga tgaagatgaa ataattgata
     6841 acttgtcaaa aggaaacatt gcaagagtaa aattaatact tggattttgc caagacccag
     6901 agtacatact tcttgatgaa ccatttacag gaattgattt gtttaagagg gaagagttta
     6961 taggagtaat agcgcaatat atggaggaaa atcaagccat tatcataact actcatgaga
     7021 tagtagagat agaaagttta gttgatgagg ttgtaattct tgatgaaggg cagattataa
     7081 cttcatttaa tgcagaagaa ttaagagaaa gagaaggtaa atctatatta gataagatga
     7141 gggaggtata taagaatgaa taaacttcta tatctaataa atgtagattt gagaagaagc
     7201 aataagtttt atatagctta tatatcattt ttttcattaa ttacacttag tttaaattta
     7261 ttcgaaatta ataaatttaa aaattctaaa tttattatga acttagcaat agaagatttt
     7321 ggaggtatat tttatggact agggatactt agtaatcttg gttttatcca aaatatattg
     7381 ttctttggaa tgttgggact ttttatatac tatattttta tgtggaaaag agagttcttt
     7441 tcaaataatc aaagtattta tacacttatg atgttacctc aaaataagtt taagatttgt
     7501 atatctaaat caatagtttt gataacaatg atatatggtt ttttgataag tcagacagca
     7561 acattattta tatgtaaata tatatttaat ttcattttta gaaatattcc tataatcaat
     7621 atgaactttg ccaaagattt atattattta aacttgaaga gtataccaat tgattttatc
     7681 tcttttatgg ctatttatgt atttttacta cttatagcta tttctattgt aaattgctgt
     7741 atat
//
LOCUS       DAMJWP010000171         7720 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.177, whole genome shotgun sequence.
ACCESSION   DAMJWP010000171 DAMJWP010000000
VERSION     DAMJWP010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7720
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.177"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(<1..1329)
                     /locus_tag="PC700_002852"
     CDS             complement(<1..1329)
                     /locus_tag="PC700_002852"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HDF2582131.1"
                     /translation="MINIEIPKKVDYIIKELETNGYEAYVVGGCVRDCLLERVPNDWD
                     ITTSARPEVVVELFEKTIPTGIQHGTVTVMIEHEPFEVTTYRIDGNYSDGRHPDSIEF
                     TNNIVKDLSRRDFTINSIAYNSKTGLVDPFNGYEDIQNKYIRCVGNPVDRFEEDALRM
                     LRAVRFSAQLNFKIAEGTKQSIHKKADLIKNVSIERIQTEFNKILVSDSSKLNLLKST
                     GLLKFIIPEICELEDVTQHNPYHIYDVQKHTLISTEVIEDELYLKLTMLFHDLGKKVT
                     KTTDKNGVDHFYTHSRESVKIAKKILRRLKYDNYTINKVLILIQYHDYRIEPKRKIIK
                     KLLNKLEDVELFEDLIKVNWADTLAKNPKYAKQKILNLIECEKEFKHIINQKECFNLK
                     DLAINGKDLISIGIKPGKDIGHILNKMLEIVINNPELNEKEILKEKALNIY"
     gene            complement(1480..3285)
                     /gene="lepA"
                     /locus_tag="PC700_002853"
     CDS             complement(1480..3285)
                     /gene="lepA"
                     /locus_tag="PC700_002853"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDF2582132.1"
                     /translation="MDNKQSRTRNFSIIAHIDHGKSTLADRLIQQTGLVSERDMKSQL
                     LDNMDLERERGITIKLQNIRLMYKAKDGNEYYLNLIDTPGHVDFNYEVSRSLAACEGA
                     LLVVDAAQGVEAQTLANVYLAIDQDLEILPIINKIDLPSARPEEVKNEIEDLIGLDSS
                     EAPLISAKTGLNIEDVLEDIVKNVPPPKGDNEAPLKALIFDSYYDAYKGVVAYVRVFE
                     GTVKKGMTIKMMNTNKKFEVTEVGVMAPGQTELSELSAGDVGYIAASIKDIRSCRVGD
                     TITDSNNPTEEPLPGYKKATPMVYCGIYPGEGEKYENVRDALEKLQVNDAALEYEAET
                     SAALGFGFRCGFLGLLHMEIMQERLEREFNLDIITTAPSVIYRVTKMDGEVVMIQNPA
                     NLPEPSEIKMIEEPIVKGDIIVPKDYVGVVMELCQERRGNMLNMEYIDERRVMLHYDL
                     PLNEVVYDFFDALKSRTRGYGSLDYEVKGYVASTLVKLDILINKEQVDALSFIVHETR
                     AFPRGKAMCEKLKGEIPRHQFAIPIQAAVGNKVIARETISALRKDVLAKCYGGDISRK
                     KKLLEKQKEGKKRMRQIGSVEVPQKAFMSVLKLDE"
     gene            complement(3567..3818)
                     /locus_tag="PC700_002854"
     CDS             complement(3567..3818)
                     /locus_tag="PC700_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582133.1"
                     /translation="MSRLEKNIESKSKLRKYKYIMRILFLITMIIGTSVCVFIVDSNA
                     KSMLGMKQYDFPSIKFDITKFDFDIPNFDIHEIIKKFNK"
     gene            complement(3849..4868)
                     /locus_tag="PC700_002855"
     CDS             complement(3849..4868)
                     /locus_tag="PC700_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HDF2582134.1"
                     /translation="MFKKCIKVVTLTFILACILPGKSLALNQDDFLKFLVNSSYPEAK
                     VEGNDTENKKNNKNKETSKENKEESKEENTKSKDASKVDNKKESEKEYIKLYVGKENV
                     PDIESKNSDTTETNTTSSSDYKDDLRVTKENPRILIYHTHGCETYSNSPDGNYHSRDK
                     KNSVMEVGSALTSALDSKGWGVVHTTKYHDYPSYNNSYASSLKTIQSILPKYNSVDIA
                     IDLHRDARDLTNPATKEKDHLKYTTMINGERVSKFFFVVGGKNTNRKQLRALAEDITA
                     FAEKKYPGLVSPIVEKDYARFNQFAVKNHMLVEIGNNATSVEESKATTKYLAEILDEY
                     FKQKN"
     gene            complement(4886..5839)
                     /locus_tag="PC700_002856"
     CDS             complement(4886..5839)
                     /locus_tag="PC700_002856"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HDF2582135.1"
                     /translation="MINVRTDLALEASEMCEKSQEGSSIPGVKIETKELENCIVTKVE
                     VIDRQGAEIMNKDIGKYITLESNLMKFDDDESREEMISYLKEELVDIFGQDKNKKTLV
                     IGLGNRNITSDALGPKSVSKTLVTRHLFKNYNKDYDDDFTEVSALSPGVMGVTGIETS
                     EIVKSLVEKVKPDRVVAIDALASRKMERVNSTIQISTAGISPGGGVGNTRKSLTKETL
                     GVDVIAIGVPTVVDAATLTIDVLDMAIDNLIAQSEETESFYEMLKKLKEEEKYHLIKD
                     SLDPYDKNLIVTPKDIDDTIENLSIIISEGLNRSLHPGRLV"
     gene            complement(6182..6958)
                     /locus_tag="PC700_002857"
     CDS             complement(6182..6958)
                     /locus_tag="PC700_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2582136.1"
                     /translation="MKFHEFGDKNKPHIMLIHGGGNAWWNYLRQAEVLSNNYHVILPT
                     LDGHGEEYETTYISTEDTADKLISYIDENCSGHLFALCGVSLGGQIVMELLSRKPNIT
                     KKAIIDGSICYPRPLMARFCIAGIRFFGGLLFSEKACRFQIAMMPKLLPENMQYPDEI
                     KAYYMTDMPHVRKETFYNMYRTYMMNYTLKENVKKTTAQVMYWYGEKEMKCVKKSAKM
                     FQSYVPSCEIYEAKGYNHGYLSLYLPNEWLEIAEPFFQKE"
     gene            complement(6992..7513)
                     /locus_tag="PC700_002858"
     CDS             complement(6992..7513)
                     /locus_tag="PC700_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2582137.1"
                     /translation="MDIAKLILNPARLRILQYIRLHGSARTSDIVKYLNDIPRATVYH
                     HVKILEENNMIEVIKENRVRGTIEKVYALKEYTTSMEGETFVALSTAFYVGLMQEMNE
                     YFSRKEQDHKKDNVFFSSALLYMSDNEYENLLKSIADLLKPYIEQKPKSNLKLRKLSI
                     ISSPPVVDNDKTK"
BASE COUNT         2376 a         1385 c          875 g         3084 t
ORIGIN      
        1 atatatattt aatgcttttt cttttagtat ttctttttca tttaattctg gattatttat
       61 gactatttca agcatcttat ttaaaatatg tcctatatct ttacctggtt ttatacctat
      121 agatatcaaa tcttttccat taatagctaa atctttaaga ttaaagcact ccttttgatt
      181 tattatatgt ttaaattctt tttcacactc aataagattt aatatctttt gttttgcata
      241 ctttgggttt ttggcaagtg tatcagccca attaactttt attaaatctt caaataattc
      301 aacatcttca agtttattta ataacttttt aattatcttc cttttaggtt ctattctata
      361 atcatgatat tgtattaata ttaaaacttt attaatagta taattatcat acttaagtct
      421 tctcaaaatc ttctttgcaa tctttacaga ttctctacta tgagtataaa aatggtcaac
      481 accattttta tcagttgttt tagttacttt tttacctaaa tcatgaaaca acattgtaag
      541 ttttaaatac agttcatctt caattacttc tgtagatatt aaagtatgtt tttgtacatc
      601 atagatgtga tatggattgt gttgagtaac atcttctaat tcacatattt caggaattat
      661 aaatttcaac aaacctgttg attttaataa gtttaattta gatgaatcag atactaatat
      721 tttattaaac tctgtctgga ttctttcgat agaaacattt tttatcaaat ctgctttttt
      781 atgaatactt tgcttagtac cttcagctat tttaaaatta agttgtgctg aaaatctaac
      841 tgccctaagc attctaagtg catcttcttc aaatctatct actggatttc caacacaacg
      901 tatatactta ttttgtatat cttcatagcc attaaatggg tcgactaatc ctgtctttga
      961 attgtatgca attgaattga tagtaaaatc tcttcttgac aagtccttaa ctatgttatt
     1021 tgtaaattct attgaatctg gatgtctacc atcactataa tttccatcaa ttctataagt
     1081 tgtcacttca aatggttcat gttcaatcat aactgttaca gtcccatgtt gtataccagt
     1141 tggaattgtt ttttcaaata attctactac cacttctggt ctagcactag ttgttatatc
     1201 ccaatcgttt ggaactcttt ctaataaaca atctctaacg catcctccaa ctacataagc
     1261 ttcataacca ttcgtttcta attctttaat tatatagtct acttttttag gaatttctat
     1321 attaatcata aatatcacct ttttattctt ctttaatata ttcttactta tattctctat
     1381 gagtttactt aaaatatacc acaaaaaagc tagattaatt tagaaataat tctaaaatca
     1441 ctctagcttt taaatatatt tatctaaatt tataagaatt tattcatcta gtttaagtac
     1501 agacataaat gctttttgag gaacttcaac agaaccaatt tgtctcatac gcttcttacc
     1561 ttctttttgc ttttcaagaa gtttcttctt acgagatata tccccaccat aacatttagc
     1621 aagaacatct tttcttagag cgcttattgt ttctcttgca ataaccttat taccaacagc
     1681 agcttgtata ggtatagcaa actgatgtct tggaatttct ccttttaatt tctcacacat
     1741 tgcctttcct ctagggaaag cacgtgtctc atggacaata aaactaagtg catccacttg
     1801 ttctttattg attaatatat caagttttac aagtgtagat gctacataac ctttaacctc
     1861 ataatcaaga gaaccataac ctctagttct cgattttaaa gcatcaaaga aatcgtaaac
     1921 aacttcattg agaggaaggt catagtgaag cattactctt ctctcatcta tatattccat
     1981 atttaacata tttccacgtc tttcctggca aagctccata acaacaccca catagtcttt
     2041 aggaactatt atatctcctt ttactatagg ctcttctatc atctttatct cagatggttc
     2101 tggtaagttt gcaggatttt gtatcattac aacctctcca tccatttttg taactctata
     2161 tataacagat ggagctgtag taattatatc aaggttaaat tctctctcaa gtctttcttg
     2221 cattatctcc atatgtaaaa gacctaagaa accacatcta aaaccaaatc ctagtgctgc
     2281 tgaagtctca gcttcatatt caagtgcagc atcatttact tgaagctttt caagagcatc
     2341 tctaacattc tcatactttt ctccttctcc tggatatata ccacaatata ccattggagt
     2401 agcttttttg tatcctggta aaggttcttc tgtaggatta tttgaatctg ttattgtatc
     2461 accaacacga caacttctta tatcttttat actagcagca atataaccaa catcacctgc
     2521 tgataattca ctaagttcag tttgacctgg agccataaca ccaacttcag ttacctcaaa
     2581 ctttttattg gtattcatca tttttattgt catacccttt ttaacagttc cttcaaatac
     2641 tctaacataa gctacaacac ctttgtaagc atcatagtaa gaatcgaata ttaatgcctt
     2701 taatggagct tcattatctc ccttaggtgg aggaacattt tttacaatat cttctaaaac
     2761 atcttctatg tttaaaccag ttttagctga aataagagga gcttcacttg aatcaagccc
     2821 tattaaatct tctatttcat tttttacttc ctctggtctt gcacttggta aatctatttt
     2881 atttattatt ggtaatattt ctaaatcttg gtctatagca agataaacat tggctaaagt
     2941 ttgtgcctca acaccttgag ctgcatcaac tactaatagt gctccttcac atgcagctaa
     3001 acttcttgat acctcatagt taaagtctac atgtccaggt gtatctataa gatttaaata
     3061 atattcattt ccatccttcg ctttatacat taatcttatg ttttgaagct ttatagtgat
     3121 tcctctttct ctttcaaggt ccatattgtc aagtaattga cttttcatgt ctctttcact
     3181 aacaagccca gtttgttgta ttaatctatc agctaaggta gactttccat ggtctatatg
     3241 tgcaattata ctaaaatttc tagttctact ttgtttattg tccaccttaa aattccctcc
     3301 tcttgattat tcttatagtt attattttac attatctaaa aactctttat ataccaaatt
     3361 taaaattttt gaatataaac taattggcac taaacaaaat tatccacatt atatgtggat
     3421 tatcaagtct aacactaaat attatataat atcgtaaact taaagtaaat cctataaaat
     3481 acagttaatg aatttttatt ttatacatta aaaatatata actaaaataa ggcttatttt
     3541 aatcaataaa ccttattcac ttattactac ttattaaatt tttttatgat ttcatgtata
     3601 tcaaaatttg gtatgtcaaa atcaaatttt gttatatcaa atttaatact tggaaaatca
     3661 tattgtttca ttcctagcat actttttgca ttactgtcta ctataaatac acaaactgat
     3721 gtacctataa tcatagtaat caaaaataag attctcataa tatacttgta ttttcttagt
     3781 ttacttttac tttctatatt tttttctaat cggctcattt agcatgctcc taattataac
     3841 ttaaagctct aatttttttg tttaaaatat tcatctaaaa tctcggctaa atactttgta
     3901 gtagccttag attcttctac acttgttgcg ttattcccta tttctactaa catatgattc
     3961 ttaactgcaa actgattgaa tctagcatag tctttttcta ctattggaga tactagacca
     4021 ggatactttt tctcagcaaa agcagttata tcttctgcta aagctctaag ttgttttcta
     4081 tttgtatttt tacctcctac aacaaagaag aactttgata ctctctctcc atttatcata
     4141 gttgtatatt ttaaatggtc tttttcctta gtggctggat tagttaaatc cctagcatct
     4201 ctatgtaagt ctatcgctat atccactgag ttatatttag gtaatatact ttgtattgtt
     4261 tttaagctac ttgcataaga gttattataa gatggatagt catggtattt agtagtatgg
     4321 actacacccc atcctttgct atctaaagca ctagttaatg cacttcctac ttccattact
     4381 gaatttttct tatctcttga atgataattt ccatcaggtg aatttgaata agtctcacat
     4441 ccatgagtat gatatatcaa aattcttgga ttttctttgg ttactcttaa atcatcctta
     4501 taatctgaac tagatgttgt gttagtttca gttgtgtctg aattttttga ctcaatatct
     4561 ggaacatttt ctttaccaac ataaagtttt atatattctt tttcactctc ttttttatta
     4621 tcaactttac ttgcatcttt agatttagtg ttttcttctt tactttcttc cttattttct
     4681 ttgcttgttt ctttattttt attattcttt ttattttctg tatcatttcc ttcgacttta
     4741 gcttctggat aagatgaatt tactaaaaac tttaaaaaat catcctgatt caaagctaat
     4801 gattttcctg gtaaaatgca tgctaatata aaagtcaagg ttactacttt aatgcatttt
     4861 ttaaacataa ttgctcctcc ttattttata ccaatctacc tggatgtaaa gacctattta
     4921 agccttcact tattattata gatagatttt cgattgtatc atctatatcc tttggtgtta
     4981 ctattaagtt cttgtcatat gggtctaacg aatcttttat tagatgatat ttttcttctt
     5041 ctttaagttt tttcaacatt tcataaaaac tttctgtttc ttctgattga gctattaaat
     5101 tgtctattgc catatctaag acatctattg taagagttgc tgcatcaacc accgttggaa
     5161 cgcctattgc aataacatcc acgcctaaag tttctttagt tagagatttt ctagtattgc
     5221 caacacctcc accaggtgat attcctgctg ttgatatctg tatagtagag tttactcttt
     5281 ccatttttct tgaagccaat gcgtctattg ctactactct atctggtttt actttttcaa
     5341 ccaatgactt tactatttca ctagtttcta ttccagtaac acccataact cctgggctta
     5401 aagctgatac ctcagtgaaa tcatcatcat agtctttatt ataattttta aaaagatgtc
     5461 tagttacaag tgtttttgat acactctttg gtcctaacgc atcagaagta atatttctat
     5521 ttccaagacc aattaccagg gtttttttat ttttgtcttg gccaaatata tctactaact
     5581 cctcttttaa ataacttatc atttcttctc tcgactcatc atcatcaaac ttcataagat
     5641 tactttccag agttatatac tttcctatat ctttattcat tatctctgcg ccttgcctgt
     5701 ctattacttc aactttagtt acaatgcaat tttctaattc tttagtttct atttttacac
     5761 caggaatact acttccttct tgagactttt cacacatttc actagcttct aaagctaaat
     5821 ctgttcttac attaatcata taaacctcca gtataaatta aatgctaatt ataattgtta
     5881 acaaaattta gtattttata acaagaaatt gtaaatttat ttaaaacaag taaaattaag
     5941 gctttcaatg taagaaaatt gggtttagtc agtgatttgt cagtaaactt tcttatctat
     6001 catttattta caagcaaaaa aaggtagcaa ctatttatat aggcgctacc tttaatctat
     6061 ttataatttt cctattaagc tttcactaat atattaacta aaataacttt tttatattta
     6121 tacctaattt atttactata taaaaaaaca gcaataatta tacaataatc acctaacacc
     6181 tttattcctt ttggaaaaat ggttctgcaa tttccaacca ttcatttgga agataaagcg
     6241 ataaataccc atgattatag cccttagctt cataaatctc acaagagggt acataagact
     6301 gaaacatttt tgcagatttt ttaacgcact tcatttcttt ttctccatac cagtacatta
     6361 cttgtgctgt ggttttttta acattctctt taagtgtata gttcatcata taagtacgat
     6421 acatattata gaatgtttct tttcttacat gtggcatatc tgtcatataa tacgctttta
     6481 tttcatctgg atattgcata ttttcaggta acaattttgg catcatagca atttgaaagc
     6541 gacaagcctt ctcactaaac aaaagtcctc caaaaaatcg aataccagca atacaaaacc
     6601 ttgccataag aggtcttggg tagcaaatac taccatcaat aattgctttt ttagtaatat
     6661 taggttttcg agataaaagt tccatgacaa tttgaccacc aagtgatacc ccacacaaag
     6721 cgaaaagatg tccactgcaa ttttcatcta tatatgatat caacttatct gctgtatcct
     6781 ctgtagaaat gtatgttgtt tcatattctt ctccatgacc atccaatgta ggaagtatca
     6841 catgatagtt gttagacaaa acttctgctt gtcgcaaata gttccaccac gcattccctc
     6901 ccccatgaat caacataata tgtggtttgt ttttatctcc aaattcatga aatttcatat
     6961 acaacctcct tttataaaaa taagattcat gttattttgt tttatcatta tcaaccacag
     7021 gtggagaaga tataattgaa agttttctaa gttttagatt tgatttaggc ttttgttcta
     7081 tatatggttt caataaatca gctatacttt taagaagatt ttcatattca ttatcactca
     7141 tatatagtaa tgctgaagag aaaaatacat tatccttttt atggtcttgc tcttttctgc
     7201 taaagtattc attcatttcc tgcatcagac ctacataaaa tgctgtagaa agtgcaacga
     7261 aggtctctcc ttccatagat gttgtatatt cctttagtgc ataaaccttt tcaatagtac
     7321 cacgcacacg attttcttta attacttcaa tcatattgtt ctcttctaaa atttttacat
     7381 ggtgataaac tgttgcacga ggaatatcat ttaaatactt tacaatatca gatgtgcgag
     7441 cactgccatg aagtcgaata tattgtaaga tacgaagcct tgcaggattt aaaattagtt
     7501 ttgctatgtc catcttatca gccctttctt tagtctataa aaatattata gtctattttt
     7561 ttagactatt caagtctatg atataagtag taattacaac tataataaat gctacaaatg
     7621 ctattctata taaacactga gattaattac atataacctt ctaacatatt agttatattg
     7681 tttgatgcat ctatcataca tactaatgaa aaaaattatc
//
LOCUS       DAMJWP010000172         7674 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.179, whole genome shotgun sequence.
ACCESSION   DAMJWP010000172 DAMJWP010000000
VERSION     DAMJWP010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7674
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.179"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            107..2335
                     /locus_tag="PC700_002859"
     CDS             107..2335
                     /locus_tag="PC700_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HDF2582138.1"
                     /translation="MRHLKKTNSLKEILNWNNIKNLRPSKGLKNILEIFKSDLKDIRG
                     NYAVLVIVLGLAFLPSLYAWFNIKASWDPYGSTANMLVAVTNEDKGNEIAGQKINVGD
                     QLIEQLKDNKSLGWQFVDEKTALEGVKKGTYYASLKIPKNFTKDLTSLITDDVKKGEI
                     IYTVNEKINAVAPKITDKGASTIQLQVNQTIVKTVSEIVLGISNGVGAGIEHSLPKLT
                     KVENSLIDLQKRFDHINKTVDLASDATSKLSDTVKDIKSDLPTIKKTLNDTKLLSSDL
                     KKFLEDTNDNLDELSPLIKSDLNLMVDLSSSASSLTLNLIDAVNSGSEDVPKLIDNLS
                     EKLSNLQSLNDTLVDFLTKLNQLTSNNRLDDVIDNLEDSSNKIDSSISTLNDIKNKVI
                     SGQQPSISALNNVLSLSNGIGRINLNILNNFDSKISKPINSIFANSIKVANDIITVLD
                     KAEAKLPKVEEILTTSLKLSGNAQESISLIRERLPLAKGMLDDLIDTLSKISNGEDMK
                     KLVSLLESDILTKSNFLKEPVEVKTEKLYPIENYGSAMTPFYSVLSTWVGILLISALL
                     STSVHGNYKPYEIYFGRGLTFLSIALVQGFIIGTGDILLLGVTAKHPILLIVLLMFTS
                     MVFNFIVYSLVSVFGNVGKAIAIVLLVVQIGSSGGTFPIQVTPTFFQRINPIMPFTYA
                     IGATREAVGGIYAPNLTKDILVLLLFMILSICLNVLLKGPINRVIKPFNDKFGDSNLT
                     GH"
     gene            complement(2573..2872)
                     /locus_tag="PC700_002860"
     CDS             complement(2573..2872)
                     /locus_tag="PC700_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDF2582139.1"
                     /translation="MYKNINKKQLKEMMKNEKDVLLLDVRTKDEFKEYKIENAINISL
                     QDLINNIDEIYDYKDKKVVVYCRSGHRSVTACNLLAEEGFEHLYNLNSGIIDYMS"
     gene            3079..6120
                     /locus_tag="PC700_002861"
     CDS             3079..6120
                     /locus_tag="PC700_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp20"
                     /protein_id="HDF2582140.1"
                     /translation="MFKQRISKLLSSTLVLSMLFTAAPNITFADNTKDNSEKYQSSDI
                     ELHDYSKNAESYTKTKALAKEKIQTLLSKYGAVSAQYALIDNGKIEISGNGGVYSKQD
                     NKNLNKDNMYSIASISKMFTTTAVMKLVDDGKLNLDTPVVKYIPEFKMADDRYKEITP
                     RMLLNHSSGLMGSSFKNTILLADNDSYGHDNFLKELQKQRLKAKPGAFSVYCNDGFTL
                     AEILVERVSGMSFTNFLDKYINNPLNLQNTKTTENSFDSSKLAKAYVPYWEDAVPQDN
                     LNAIGAGGLYSSAENLCTFAQTFMKNSNGILSPASVKAMENKEYLNGLWPEGEDSILG
                     YGLGWDCVNTYPFNQYNLKALTKGGDSLLFHSNLIVLPDENMAVAVLSSGGSSQLNEI
                     IGQEILLSALKEKGKIKEIKPDKTFSKPQQVKMPSSLKENSGLYASSNMIKVDVNDNG
                     TLTVSSPYIENGPEDKYVYIGQDRFVSEKGNSCLKFVKEKNNITYLNMSSYDDVPGLG
                     QTASLYYVAQKVDDNNISNSVKEVWKKRSGKGYYLVDEKYTSQSYMFGSVKASFSLSD
                     ETPGYIVNTKIMDENNSNAFIEIPGVIGRDLSDIKLHKENGTEYLSFGTLTYVSEDSI
                     TNLPAEKSFTCELESNGYAKWYKIGDDIANKKIEVNLPQNSAFAVYDDKGVPVNYSLV
                     TKNNRVRLPKGGVIVFLGSPNARFEVTYQDEVNASALTGTDRYETSIKISQAGWENAE
                     NAVLINDSAIADALAATPFAYKKNAPILLTGSSQINEKTLAELKRLKVKNVYVVGGEA
                     SINEKSLDTIKSNNISVSRISGSDRYQTSMNIAKELNNISNISKISVVNGEKGLADAV
                     SIGAVSAQNDMPIILTNENSNITEINNLFKNKKIDKSYVIGGEYTVSKNIESKLQNPQ
                     RISGNTRNETNAKVIKEFYKDSKIDNLYVAKNGMNKQDDLIDGLSVGVLAGKTKSPVM
                     LVGNSLDYNQKELFKTMRFKSVTQIGGNGNENSFKQIKEIA"
     gene            complement(6281..7555)
                     /locus_tag="PC700_002862"
     CDS             complement(6281..7555)
                     /locus_tag="PC700_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2582141.1"
                     /translation="MITISHITAKNMYKSLEERINKFPQGAPPSDTLYKILNVLYTEQ
                     EAKLVAQLPIKPFRVKTAAKIWSVSESEAYRVLDKLASKALILDIEDNKGKKYIMPPP
                     MAGFFEFAMMRTRHDIDQKLLAELYYQYMNVEEDFIKDLFYSTETKLGRVYVQEEVLT
                     NDNEVSILDYERATHIIDESTHIGISMCYCRHRMQHVGKACDAPMDICMTFDNVANSL
                     INNKFARRVDKIECKELLHQAYEHNLVQCGENVRKGVTFICNCCGCCCEAMVAAKRFG
                     NLHPVQTTSFIPNINHENCVKCGKCITACPIDAISKVKEDGKEYIKIDEDRCLGCGVC
                     VRNCHKNSIMLLKRDEKIITPANSVHRAVLMAIEKGQLQNLIFDNNALASHRAMGAIL
                     SAILKLEPAKKILASKQLKSVYLDKLLSMNDK"
BASE COUNT         2837 a         1073 c         1103 g         2661 t
ORIGIN      
        1 tattaaaatt aagacaaaat tttaacttta ctatttgtgt aaaaagtaat ataatttaaa
       61 attgattata aatctaactt ttttctttat caaaatattt taggaggtga gacatttgaa
      121 aaaaactaat agtttaaaag aaattttaaa ctggaataat atcaagaatt taagaccttc
      181 aaagggttta aaaaatatat tagaaatttt taaaagtgac ctaaaagata tacgtggcaa
      241 ttatgccgta cttgtaatag ttttaggtct tgctttttta ccatctctgt atgcttggtt
      301 caatataaag gcatcttggg acccttatgg aagcactgct aatatgcttg tagcagtaac
      361 aaatgaagat aaaggaaatg aaatagcagg acaaaagatt aatgtaggag accaattaat
      421 tgaacaactc aaagataaca agagtttagg ttggcagttt gtagatgaaa aaactgctct
      481 tgaaggtgtt aaaaaaggta cttattatgc atcacttaag attccaaaaa attttacaaa
      541 agatttgaca tctttaatta cagatgatgt taaaaaaggc gaaattatct atactgttaa
      601 tgaaaaaata aatgcagttg caccaaagat tacagataaa ggtgcttcta caatacaact
      661 acaggttaat cagactatag taaaaacagt tagtgaaatc gttcttggta tatcaaacgg
      721 agttggtgca ggaattgaac acagtcttcc aaaattgaca aaagttgaaa attcccttat
      781 tgaccttcaa aagagatttg accatataaa taagactgtg gatttagctt ctgatgctac
      841 ttcaaaatta tctgatacag ttaaagatat taaatctgat ttacctacaa ttaaaaagac
      901 tctgaatgat acaaaactat tatcaagtga cttaaaaaaa ttcttagagg atacaaatga
      961 taatttagac gaactttctc ctcttattaa gtctgactta aatttaatgg ttgacctttc
     1021 atcttctgct tcttctctta cattaaattt aattgatgca gtaaatagtg gcagtgaaga
     1081 tgtacctaag ttgatagata atttatcaga aaaactttct aatttgcaat ctttaaacga
     1141 tactttggtg gactttttaa ctaaattaaa ccaacttaca tcaaataata gattggatga
     1201 tgtaatagat aatcttgaag actctagcaa taaaatagat tcatctatat caacattaaa
     1261 tgatattaaa aacaaagtta tatctggaca acaaccatct ataagtgctt tgaataatgt
     1321 tttaagtcta tcaaatggta ttggaagaat taatttaaat attttaaata attttgactc
     1381 aaaaatatca aaacctataa atagtatatt tgcaaacagt ataaaagtag caaatgatat
     1441 aattactgtt ttagataagg cagaagcaaa acttccaaag gttgaagaaa tacttactac
     1501 ttctctaaaa ctttcaggaa atgcacagga aagtatatca ttgataagag aaagacttcc
     1561 tttagcaaaa ggaatgttgg atgatttgat tgatacttta agcaaaatta gtaatggtga
     1621 agatatgaaa aaattagtca gcttgcttga aagtgatata ttgacaaagt ctaatttctt
     1681 aaaagaacct gttgaggtga aaacagagaa attatatcca attgaaaatt atggttctgc
     1741 aatgacacca ttttatagtg ttctttctac atgggttggt atattactta tttcagcact
     1801 attgtctaca tctgtacatg gaaactataa accttatgaa atttactttg gaagaggtct
     1861 tactttttta agtatagctc ttgtccaagg ttttataata ggaactggag atatattatt
     1921 gttaggagta acagcaaaac atccaatttt attgatagtt ctgcttatgt ttactagtat
     1981 ggtatttaac tttatagtgt attctttagt ttctgtattt ggaaatgtag gaaaagcaat
     2041 tgcaatagta ctattagtcg ttcagattgg ttcttctggt ggtacattcc ctatacaagt
     2101 tacacctaca ttcttccaaa gaataaaccc tataatgcca tttacctatg caataggtgc
     2161 tacaagggaa gcagttggtg gtatatatgc tccaaatcta actaaagata tactagttct
     2221 attactattt atgattctgt ctatttgttt aaacgtactt ctaaaaggac ctataaatag
     2281 agttataaaa ccatttaatg ataaatttgg cgatagtaat ttaactggtc attaaaaaat
     2341 taattctgtc tttaaatata tttttttaat aatattctaa gttcatatta aaaggtagta
     2401 gctaaaaagc tactaccttt ttccaactta aaaactaata tttactacaa aaactgtcat
     2461 agaaaaaagc gaaaaaattc gttctttcct aaatttatct tataaatatt tagatatatt
     2521 cctacttaat aaacagagct atacatagta tttaatgtat aacttacctt atttaactca
     2581 tataatcaat tattcctgaa tttaaattat ataaatgctc aaaaccttct tcagctaaca
     2641 aattacatgc agtaacactt ctatgaccac ttctgcaata aactactact ttcttatctt
     2701 tataatcgta aatttcatct atattattga ttaaatcttg taaagatata tttatagcat
     2761 tttctatttt atattcctta aattcatctt tagttcttac atctaataat aaaacatcct
     2821 tttcattttt catcatttct ttaagttgct tcttatttat attcttatac atatgtctaa
     2881 aattcctcca ttttatttat atattaccta taccctttat tctttataca acaacaaata
     2941 ttatttttat atttttctct gttatataaa aagctatact tataaaagct ttttttattg
     3001 ataataatct gttgattaaa ataattaaga atgttatgct aattataaat tattaaaaag
     3061 ggaaagaggt aatgtaatat gtttaagcaa aggatctcta aattattatc tagtacatta
     3121 gtattgagta tgttatttac tgctgctcca aatattactt ttgctgataa tacaaaagat
     3181 aattccgaaa aatatcaaag ttctgatata gaattacatg attatagtaa aaacgcggaa
     3241 tcttacacaa aaactaaagc tcttgctaaa gagaaaatcc aaactttgct atcaaagtat
     3301 ggtgctgtaa gtgctcaata tgctttgatt gataatggca aaattgagat ttctggtaat
     3361 ggtggagttt acagcaaaca ggataacaaa aacttaaata aggataatat gtatagtatc
     3421 gcatctatca gtaaaatgtt tactacaaca gctgttatga agttggttga tgatggaaaa
     3481 ttaaatttag atactcctgt tgtaaagtat atacctgaat ttaaaatggc tgatgataga
     3541 tacaaggaaa tcactccaag aatgttatta aatcattcat cagggttgat gggaagtagt
     3601 tttaaaaaca ctattttatt agcagacaat gattcttatg gtcatgataa tttcttaaaa
     3661 gaattacaaa aacaaagatt aaaagctaaa ccaggagctt tttccgttta ttgtaatgat
     3721 ggatttactt tagctgaaat tttagtagaa agagtatctg gtatgtcatt tactaatttt
     3781 cttgataaat acataaataa tccacttaac ttacaaaata caaaaactac agaaaatagt
     3841 tttgatagtt caaaactagc aaaagcatat gtaccttatt gggaagatgc agttccacaa
     3901 gataacctta atgctatagg tgcaggtggt ttatattcaa gtgcagaaaa tctatgtaca
     3961 tttgcacaaa catttatgaa aaattcaaat ggtattcttt ctcctgcttc agtaaaagct
     4021 atggaaaaca aagaatattt aaatggtcta tggcctgaag gagaagattc tatacttgga
     4081 tatggtttag ggtgggattg tgtcaacact tatccattta atcagtataa tttaaaagct
     4141 cttacaaaag gtggagactc tttattattc cacagtaatc ttatagttct tccagatgaa
     4201 aatatggcag ttgcagttct ttcttctggt ggaagtagtc aacttaacga aataattgga
     4261 caagaaatct tactatctgc attaaaagaa aaaggtaaaa ttaaagaaat aaaacctgat
     4321 aaaacattta gtaagccaca acaagttaaa atgcctagct ctctaaaaga aaatagtggt
     4381 ctttatgctt cttcaaatat gataaaagta gatgttaatg ataatggtac tttaacagta
     4441 tcttctcctt atatagaaaa tggacctgaa gataaatacg tatacatagg tcaagataga
     4501 tttgtatctg aaaagggtaa cagttgttta aaatttgtaa aagaaaaaaa taatatcaca
     4561 tatttaaata tgagctctta tgatgatgtc cctggattag gtcagacagc aagtttatac
     4621 tatgtagctc aaaaagtaga tgacaacaat atatcaaata gtgtcaaaga agtgtggaaa
     4681 aaaagaagtg gcaaaggtta ctatctagtg gatgaaaaat atacttctca aagctatatg
     4741 tttggctctg taaaagcaag ttttagtctt tctgatgaaa ctccaggtta tatagttaac
     4801 actaaaataa tggatgagaa taactcaaat gcttttatag aaatacctgg tgtaataggt
     4861 cgtgatttat ccgacattaa acttcataaa gaaaatggta cagagtattt atcttttgga
     4921 acactaacgt atgttagtga agattctata actaatttgc cagcagaaaa atcttttact
     4981 tgtgaactag aatccaatgg ttatgcaaaa tggtataaga ttggtgacga tatcgcaaat
     5041 aaaaaaatag aagttaatct tcctcaaaat tcagcttttg ctgtttatga tgataaaggt
     5101 gttcctgtaa actattcttt agttacaaag aacaatagag ttagattgcc aaaaggaggc
     5161 gtaattgttt tcttaggaag tccaaatgct agatttgagg ttacatatca agatgaagta
     5221 aatgcaagtg cacttacagg aactgataga tatgaaactt ctattaagat aagtcaagca
     5281 ggttgggaaa atgctgaaaa tgcagtttta ataaacgatt cagcaattgc agatgctttg
     5341 gccgcaacac catttgcata caagaaaaac gctcctatat tattgactgg aagctctcaa
     5401 ataaatgaaa aaactttggc tgaattaaag agattaaaag ttaaaaatgt atatgtagtt
     5461 ggtggagagg cttctataaa tgaaaaatct ttagatacta taaaatcaaa caatatctct
     5521 gtatctagaa tttcaggaag tgatagatat caaacttcta tgaacattgc taaagaattg
     5581 aacaatatta gcaatatatc aaagatttca gtagttaatg gcgaaaaagg attagccgat
     5641 gcagtaagca ttggagctgt atcagctcaa aatgatatgc ctataatact tactaatgaa
     5701 aatagtaata taactgaaat aaataactta tttaaaaaca aaaagattga taaatcttat
     5761 gtaatcggtg gagaatatac agtttctaaa aatatagaaa gtaaacttca aaatccacag
     5821 agaatatcag gtaacactag aaatgaaact aatgcaaaag taataaaaga attttacaag
     5881 gattctaaga tagacaattt atatgttgct aaaaatggaa tgaataaaca agatgattta
     5941 atagacggat tatctgttgg agttttggct ggaaaaacta aatctcctgt aatgctagta
     6001 ggaaactcac tagattataa tcaaaaagaa ctatttaaaa ctatgagatt taaatctgta
     6061 acacaaattg gtggaaatgg aaatgaaaac tcatttaagc aaataaaaga aatagcttaa
     6121 cataactaaa aaaggctgta ttcaagtaat attaattgaa tatagccttt ttttatttaa
     6181 gttataagtt ttatatgtgt aaaaattaaa tttaaatatt aaatgataag tttattaaga
     6241 tatagataag tctaaaactt tgcctttttt aagttagttt ctatttatca ttcatactta
     6301 acaatttatc taaatatact gactttaatt gtttactggc taaaatcttt ttagctggtt
     6361 caagctttaa tatagctgac agtatagctc ccatagctct atgactagcc agagcattat
     6421 tatcaaaaat taaattttgt aactgccctt tttcaattgc catgagaact gctctatgta
     6481 ctgaatttgc tggagttatt attttttcat ctctctttaa taacataatg ctatttttat
     6541 ggcaatttct aacacaaact ccacaaccta aacatctatc ctcatcaatt tttatatatt
     6601 ctttaccatc ttccttaacc ttgcttatcg catctattgg acatgcagtt atacatttac
     6661 cacactttac acaattttca tggtttatat ttggtataaa acttgttgtc tgtactggat
     6721 gaagatttcc aaatctttta gctgctacca ttgcttcaca gcaacatcca cagcaattac
     6781 atataaacgt aacccctttc ctaacatttt ctccacattg aactaagtta tgttcatatg
     6841 cttgatgaag tagttcttta cattctattt tatccacacg tcttgcaaat ttattattta
     6901 ttaaagaatt tgctacattg tcaaacgtca tacatatatc catgggagca tcacaagctt
     6961 ttccaacatg ttgcattcta tgtctacagt aacacatact tatacctata tgagttgatt
     7021 catcaattat atgagtagcc ctttcataat ctaaaatact aacctcatta tcattagtta
     7081 aaacttcctc ttgaacgtat actcttccaa gcttcgtttc tgttgaataa aataaatctt
     7141 tgataaaatc ttcttctaca ttcatatatt gatagtaaag ttctgccaaa agtttttggt
     7201 ctatatcgtg tctcgttctc atcatggcaa actcaaaaaa accagccatt ggaggaggca
     7261 ttatatattt cttcccttta ttatcttcta tatctagtat cagagcctta ctagcaagtt
     7321 tatccaatac tctatatgct tcactttcac ttacactcca aattttagct gctgttttta
     7381 ctctgaaagg ctttattgga agctgagcaa ctaacttcgc ctcttgttct gtatatagta
     7441 catttagtat tttgtataat gtatcagatg gtggagcacc ttgtggaaac ttgtttattc
     7501 tttcctctaa acttttatac atattcttcg cagttatatg agaaatagta atcaccttct
     7561 ttttcttttg ttttcaaata aaatcaaata ttctttctta ttatatattc tcttacttta
     7621 tataatacca attctttcta aatagttaaa tacctctagt aatttattat taga
//
LOCUS       DAMJWP010000173         7616 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.180, whole genome shotgun sequence.
ACCESSION   DAMJWP010000173 DAMJWP010000000
VERSION     DAMJWP010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7616
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.180"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            954..2312
                     /locus_tag="PC700_002863"
     CDS             954..2312
                     /locus_tag="PC700_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HDF2582142.1"
                     /translation="MKAKSTSKFELDGIPPLKEAIPLGLQHLMAMVIGSCLPAILIAN
                     ASGLNHKMSTLLIQAALVFAGLSTLLQLYPIPLFKGFKLGGGIPVIMGATAMFIGAGV
                     SVASKYGLPVLFGSQIIAGIVIIIIGFGLKKIRFIFTPAVSGTIVACMGLGLFPIAIK
                     NLAGGMGNETFGNPINFIVGISVSLMILGLNKYGKGLFKDASILVSIIFGYILSLILG
                     IVNFSSIQEFTLVALPKPLAFGLDIRLEVVVMFSIIYLVEIADIMGACTLSAVGGLNR
                     QVTDEELSSAVLGNGVLSTIGALFSATPMGMFGQNSAIVSNTKVVSKFVLAIGGIGLF
                     LAGISPLLANLIRTIPPCVVGGATLVIFSTLTTSGLRLVSMDGFNQENSMILGLSMAS
                     GIGFMVAPQVLEKFPKFIETLLADSSVVSGAMVAIIIQALYMVKFPGNKSDKVEDKNK
                     SL"
     gene            2418..3212
                     /locus_tag="PC700_002864"
     CDS             2418..3212
                     /locus_tag="PC700_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HDF2582143.1"
                     /translation="MVTIKEYSIPQSLEEAYKILISKKNNVILGGCGFIKLSNKNIGT
                     AIDLKDINLNYIKEDEKNILIGADTSLRSLEIDKTIKNYCSGILSSAVSNIVGVQFRQ
                     GARIGASVFSKYGFSDLIPALLVVGAKVRLYNKGILELSEFLDSELSRDILVEVILPK
                     KDAIAVFDSIRKCTGDFSVLNSAMLKENDTYKIAIGARPQKARLALEASNILNKEKDI
                     DKASIVASKELTYGSNMRASKEYRKDMASALVKRMYNAIEEGMYND"
     gene            3205..5511
                     /locus_tag="PC700_002865"
     CDS             3205..5511
                     /locus_tag="PC700_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2582144.1"
                     /translation="MINKGIRKIDSTAIVTGKPLYTEDLIMHKDVLTLKLLRSPHAHA
                     KIKNIDTSKAEKIPGVVGIYTYKDVPQVRYCVPGQTEPEASPYDRVLLEDTVRFIGDE
                     VALVAAEDEKTALKALKMIKVDYEVLKPVFDAREAEGNEVVIHPEKDIVEPYPFGLDA
                     KNNIVSKEFFEFGDVEAKLKESDFVAESSYKTQSQSHCMMETHRSYSYKDINDRIVVV
                     SSLQSPHNVKRIIRKVLGIPSSKIRVIKPRIGGGFGGKNIAATDIFTAFVTMKTGRPA
                     KFVFTRSETFVGSNSRHEMFFDVKVGADKEGNLKAIELKSLNNTGAYGGNGVSVSSES
                     GHNTLPIYNDIDAVRFNARTVYTNRLPAGALRGYGATQGIFAIDSAIDELAHKIGMDP
                     LEFRIKNIIDRYTNGKAAEDEILSCELRQCIEKGKKLINWDDKYPCKDIGNNKVRAVG
                     MAVANHGSGIPRVDMATVTIRMDEDGTYKLLSGLADLGQGADTIQAQIAAEALNTTVD
                     RISIYTGDSDLCPYDTGAFASCTTTVTGNATIKTANKLKDILLSVAESKLGFKKEDLE
                     LGKDRVFVKNDNSKFVLLEELGRESVGGIGFASDDVPVVLEATESFGMLKKIKPFIAG
                     FAEIELDKNTGEYKVINFACVPDCGTVINPTLARIQVEGGVMMGIGFAMYEDPIFDKD
                     GRLLTDSFLQYKVPTKKEVGNILVDFAENFDPGGPYGAKSLGEIVIHTPAPAIANAIF
                     NATGVRLRELPMTFEKVYMGMKNNNKQL"
     gene            5650..6927
                     /locus_tag="PC700_002866"
     CDS             5650..6927
                     /locus_tag="PC700_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2582145.1"
                     /translation="MSNRIKNIHYGTQRQQLKNLTKKEYQALREMCFLAKNLYNVGTY
                     NVRQHYFNQGEHLNYEANYHVCKENENYKLLNSNIAQQILKEVDSSFRSFFGLIKLAK
                     EGKYDFRGIKSPKYLDKEGFFSIIIGQIRIKEDGILNVPMSPAFKKLYGKVSIKVPQN
                     ILEKKIKEIRIIPKHKARFFEIQYCYEIPKSEEVSNQKNALAIDLGLENLCTCVTNLG
                     DSFIIDGKRLKSVNQWANKQNDKIESLKLKKNITAITRKQFKVWNKRNNQVKDYVNKT
                     CFYIIDYCVKNNIGNLIVGYNAKLEKSDNMERKIYRNFIRIPFGEIKGKLEYLCKIKN
                     INFIRQEESYTSKSDFFADDFLPDINVDNVKKYDFKGERITRGQYKSSVGIIINADVN
                     ASLNILKKSQVVNLSNLQNNPQILKQPQRVRIS"
     gene            7110..>7616
                     /gene="yqeC"
                     /locus_tag="PC700_002867"
     CDS             7110..>7616
                     /gene="yqeC"
                     /locus_tag="PC700_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HDF2582146.1"
                     /translation="MELSNIIEKNEIITVVGAGGKTSFINYFANFYRDKLKVLLTTTT
                     KIYVPNDYDNIIITIDGTVIPSICHGITVCGSYINNENKLVSIDSSILDEIVDQFDLV
                     LIEGDGSKRKKLKGWNAKEPVVYHKTTKTIGILDITSFGMNINEENIHRVEIFKKIAN
                     LDTSSINSS"
BASE COUNT         2970 a          836 c         1435 g         2375 t
ORIGIN      
        1 acttgtatat tttatgttgc agatacaaat atagtaaagg agagtacatt ggtacaactg
       61 agatacatag cttattaagt ttttctgtct tatgagaatt atattataaa atattcataa
      121 atattttgag tttaaattaa ataattattt tatgcttaga ttaattgata tttaattaat
      181 ttaataacaa aaacagtgaa agagtaaata atattattta cagggggtaa agcaaagtat
      241 gttaaataaa ttgtactttt ggccccgtac atagagaaat ctatgtagga cacaacttta
      301 aaaccagtaa atcctaaagc cttgcaacta caataaaaga tagaaagaaa cttttatgaa
      361 tgtggtgaaa accagaaaca atagtgagga tggtatatgg tgagagccta agtacttaaa
      421 aatggacatt tggtcgccaa gcctagaaat aggaaggtca aacgactatc tcgagagaga
      481 gtacactata agcgattgat agtggaaacg ggttgctctt agcaggtaat gctgaagatg
      541 aagaaatagt ctacgctcat atgaaagtat gaggtcacta gatttgaagt gacatctata
      601 aagtagcgtt tataggtaaa tgtttaaaac ttgaaacatg taaatactta tgaaaactta
      661 taacttataa tgattaagat tgaaagattt aaattgaatt aaatttacta taaaaaatta
      721 aaaattcata gctataatac tcttatattt gtaaagcatg gtatataatg tatacaaaat
      781 taaagaatac gtgtatttgt taataattaa atttattgca tctgtttttt aagactaaaa
      841 acttaaatat atgttggagt atagttgata tttatgaaca tcattaattt aatatattag
      901 atttttaagt atttatataa ttcaataagt tttatgtaac gggagttttt attatgaaag
      961 caaaaagtac atcaaaattt gaattagacg gaataccacc actaaaggaa gcaatacctt
     1021 tagggttaca acacttaatg gcaatggtaa taggtagttg tttaccagcg atattgattg
     1081 caaatgcatc tggacttaat cataagatgt caacattatt gattcaagcc gcattagttt
     1141 ttgcaggatt atcaacttta ttacaattat atccaatacc tttgtttaaa ggttttaaat
     1201 taggaggagg aataccagtt ataatgggag ctacagctat gtttatagga gctggggtat
     1261 ctgttgcaag taagtatgga ctaccagtat tatttggctc tcaaataata gctggtatag
     1321 taataattat aattggtttt ggtcttaaaa aaatacgatt tatatttact ccagctgtat
     1381 caggaacaat agttgcatgt atgggtcttg gactttttcc aatagctata aaaaacttag
     1441 ctgggggtat gggcaatgag acttttggga atccaattaa ttttatagtt ggaatatctg
     1501 tatctcttat gatattagga ttaaataagt atggaaaagg attatttaaa gatgcatcaa
     1561 tattagttag cataattttt ggatacattt tatcattaat tttgggtata gtaaattttt
     1621 cttcaattca ggagtttact ttggttgcgc ttccaaaacc acttgctttt gggctagata
     1681 taagacttga agttgttgta atgttttcaa taatatattt agttgagata gctgatataa
     1741 tgggtgcttg tacactttct gcggtaggag ggcttaatag acaagtaaca gatgaagaac
     1801 tttcttctgc tgttttagga aatggagtac tctctacaat aggtgcatta ttttctgcaa
     1861 ctccaatggg gatgtttgga caaaattctg caatcgtttc caatacaaag gttgtaagta
     1921 aatttgtact agctattgga ggaattggat tatttttggc aggaatatcg cctttattag
     1981 ctaatttaat aagaacaatt cctccctgtg tggttggagg agcaacactc gttatatttt
     2041 caactcttac aacatctggt ttaagacttg ttagtatgga tggatttaat caagaaaata
     2101 gcatgatatt aggtctttct atggcttctg gtattggatt catggtagct cctcaagttc
     2161 ttgaaaaatt tccaaaattt atagagacat tacttgcaga ttcaagtgta gtttcaggag
     2221 caatggtagc cataataatt caagctttat atatggtaaa atttcctgga aataaaagtg
     2281 ataaggttga ggataaaaat aaaagtttat aatgtaaagg tttaatatat gtgaattaaa
     2341 tatactacaa ttataaatat aaataaaaga tttattaaaa acttttgtgt tttaataagt
     2401 taatgatggg ggataagatg gttactataa aggaatattc aattccacag agtttagaag
     2461 aagcatataa aattttaatt tctaaaaaaa ataatgttat tttaggtgga tgtggattta
     2521 taaaattaag taataaaaat ataggaacag cgatagattt aaaagatata aatctaaatt
     2581 atataaaaga ggatgaaaaa aatattttga taggtgcaga tacttcactt agaagtttag
     2641 aaattgataa aactataaaa aattactgta gtggaatact ttcaagcgct gtttctaata
     2701 tagttggtgt tcaatttaga caaggggcta gaattggtgc aagtgtattt tcaaagtatg
     2761 gcttttctga cttaatacca gcgcttttgg ttgttggagc gaaggtgaga ttatacaata
     2821 aaggaatact agagttaagt gagtttttgg atagtgaatt aagtagggat atattggtcg
     2881 aagtaatact accaaaaaaa gatgcgatag ctgtttttga ctcaataaga aaatgcacag
     2941 gagatttttc tgtattaaat tcagcaatgc taaaagaaaa tgatacttac aaaatagcaa
     3001 ttggggcaag acctcaaaaa gctagattag cacttgaagc gagtaatata ttaaataaag
     3061 aaaaagatat agataaggca tctattgttg ctagtaagga acttacttat ggaagtaata
     3121 tgagagctag taaagagtac agaaaggata tggcaagtgc attagttaaa aggatgtata
     3181 atgctattga ggagggaatg tataatgatt aataagggga taagaaagat agatagtaca
     3241 gcaattgtca caggaaaacc tctatatact gaagacctca ttatgcataa agatgttttg
     3301 acattaaaac tacttagaag tccacatgct catgctaaga taaagaatat agatacatca
     3361 aaagccgaaa aaattccagg ggttgtgggt atttatacgt ataaagatgt accacaggtc
     3421 agatattgtg tgccaggtca aacagaacca gaagcatcac cttatgatag agttttactt
     3481 gaagatacag ttagatttat tggagatgaa gtagctttag tggctgctga agatgagaaa
     3541 acagcactta aagccttaaa aatgataaag gtagattatg aggttttaaa gcctgtgttt
     3601 gatgcaagag aagcagaagg aaatgaagtt gttatacatc cagaaaaaga catagtagag
     3661 ccataccctt ttggattgga tgcaaaaaat aatatagtat caaaagaatt ttttgaattt
     3721 ggagatgttg aagctaagct taaagaaagt gattttgtgg ctgaatcttc atacaaaacc
     3781 caaagtcaat cgcattgcat gatggaaact cataggtcat attcatataa agatataaat
     3841 gatagaattg ttgtagtttc atcattacaa tcaccacaca atgtaaagag aataattaga
     3901 aaagtattgg gtataccatc ttcaaaaata agagtaatta aacctagaat aggtggtgga
     3961 tttggaggaa agaatatagc agcaacagat atatttactg catttgttac tatgaaaact
     4021 ggtagaccag caaaatttgt atttacaaga agtgaaactt ttgtaggttc taatagtaga
     4081 catgaaatgt tttttgatgt taaagttggt gcagataaag aaggaaattt aaaagcaata
     4141 gagctaaagt cattaaacaa tacaggtgct tatggtggga atggtgtctc tgtttcttcc
     4201 gaatctggac acaatacctt accaatatat aatgatattg atgctgttag atttaatgct
     4261 agaacagtat atactaatag gttaccagca ggagcactta gagggtatgg tgctacacag
     4321 ggaatatttg caatagatag tgctattgat gaattggcac ataagattgg aatggaccca
     4381 cttgaattta gaattaaaaa tataatagat aggtatacca atggaaaagc agctgaagac
     4441 gaaatattaa gttgtgagct gagacaatgt atagaaaagg gcaagaaatt aatcaattgg
     4501 gatgataaat atccatgtaa agatatagga aacaacaaag taagagcagt aggtatggca
     4561 gtagcaaatc atggttctgg tataccgcgt gtagatatgg caacagtcac tataagaatg
     4621 gatgaagatg gcacatataa actattatct ggtcttgctg acttaggtca aggggctgat
     4681 acaattcaag ctcaaattgc tgcggaagct ttaaatacta ctgtagatag aatttctatt
     4741 tatacaggtg attcagattt atgtccctat gatacaggag catttgcttc ttgtacaaca
     4801 acggttacag gaaatgcaac aataaagaca gcaaataaat taaaagatat attactttca
     4861 gttgcagagt caaagctagg atttaaaaaa gaagatttag agttgggaaa agacagggtt
     4921 tttgttaaaa acgataatag caagtttgtt ctattagagg aattgggtag agaatctgtt
     4981 ggaggaatag gatttgctag tgatgatgta ccagtagttt tggaagcaac agaatctttt
     5041 ggcatgttga aaaaaataaa accatttata gcaggatttg cagagataga attagataaa
     5101 aatacaggcg aatataaggt cataaatttt gcatgtgtac ctgattgtgg aacagttata
     5161 aatccaactt tagctagaat acaagtagaa ggtggtgtga tgatgggtat agggtttgca
     5221 atgtatgaag accctatatt tgataaagat gggagattat taacagatag tttcctacag
     5281 tataaagttc ctacaaaaaa agaagttgga aatatattgg tagattttgc tgagaatttc
     5341 gacccaggag gtccttatgg tgctaaatca ttaggagaaa tagttataca tacaccagcg
     5401 ccagctatag caaatgcaat atttaatgct acaggagttc gtttaagaga gcttcctatg
     5461 acttttgaaa aagtttatat gggtatgaaa aataataata aacaattata ataaataata
     5521 aggtgtataa aagcaagtaa atttaaatta ttttgtaaaa taaagtaatt aaatggacat
     5581 tatatatggt gttttgtaaa aataccgtat ataatgtcta gtagagtata ttctaaagga
     5641 ggtgagttca tgtcaaacag aataaaaaat atacactatg gaactcagag acaacaatta
     5701 aaaaacttaa ctaaaaaaga atatcaagct ctacgagaga tgtgcttctt agctaagaac
     5761 ttatataatg ttggtacata taatgtaaga cagcattatt tcaatcaagg agaacatctt
     5821 aactatgaag ctaattatca tgtatgcaaa gaaaatgaga actataagct tctcaatagt
     5881 aatatagcgc aacaaatatt aaaagaagta gacagttcgt ttaggtcatt ttttgggtta
     5941 attaaattag ccaaagaagg gaaatatgac tttagaggga taaaatcacc taaatattta
     6001 gataaagaag gattttttag cataataata ggtcaaataa gaatcaaaga agatgggatt
     6061 ttaaatgtac ctatgtctcc agcatttaaa aaactatatg ggaaagtaag tataaaagtt
     6121 ccacaaaata tactagagaa aaaaattaaa gaaataagaa taatacctaa acataaagct
     6181 aggttctttg aaattcagta ttgttatgaa ataccgaaat cagaggaagt ttcaaatcaa
     6241 aaaaatgcac tagctataga cttgggttta gaaaatttat gtacatgtgt taccaattta
     6301 ggtgatagtt ttatcataga tggaaaaagg ctaaaatctg tgaatcagtg ggcaaacaaa
     6361 cagaatgata aaatagaatc cttaaaatta aaaaagaata taactgcaat aacaagaaaa
     6421 caatttaaag tttggaataa gagaaataat caagttaaag actatgtaaa taaaacctgt
     6481 ttttatataa ttgattattg tgtaaaaaat aatattggaa atttaattgt aggatataat
     6541 gctaagttag aaaaaagtga taatatggag agaaaaatat atagaaattt tataagaatc
     6601 ccatttggag agataaaagg aaaattagaa tatctatgta aaataaaaaa tatcaatttt
     6661 attaggcaag aagaaagcta tactagtaag tctgattttt ttgctgatga ttttctacct
     6721 gatatcaatg tggacaatgt taaaaaatat gactttaaag gggaaagaat aacacgtgga
     6781 cagtacaaat ccagtgttgg aatcattata aatgcagatg taaatgcgag tcttaatata
     6841 ctaaaaaaat cacaagtagt taatttatct aatttacaga ataatcctca aatattaaag
     6901 caaccacaga gagtaagaat atcttaatta tatttaaaag ataaactgat agcgtaagct
     6961 tacagcttag cggcgaattg gatccgcctt gttgtttaca acagaatcta tttactttcg
     7021 agtttatgga atttcaaaaa tcacttttac taaaaaggtc taactataat aattaagaga
     7081 ttagaaaact ggttataaga ggtgaaagtg tggaactatc aaatataata gaaaagaatg
     7141 aaataataac tgtagttgga gcaggaggaa aaacttcgtt tataaactat tttgcaaatt
     7201 tttatagaga taaattgaaa gttcttctta caacaacaac taaaatatat gtaccaaacg
     7261 attatgacaa tataattatt acaatagatg gaactgttat tccatctatt tgtcatggaa
     7321 taacagtctg tggcagttat ataaataatg aaaataaatt agtaagtatt gattcctcta
     7381 ttttggatga aattgtagac caatttgatt tagttttaat agagggagat ggctcaaaga
     7441 gaaaaaaact taaaggttgg aatgctaaag aaccagtagt atatcataaa actacaaaaa
     7501 ccatagggat attagatata acatcatttg gaatgaatat taatgaagag aatatacata
     7561 gagtagaaat atttaaaaaa atagcaaatt tagatacaag tagtattaat agtagt
//
LOCUS       DAMJWP010000174         7607 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.181, whole genome shotgun sequence.
ACCESSION   DAMJWP010000174 DAMJWP010000000
VERSION     DAMJWP010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7607
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.181"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            51..1109
                     /locus_tag="PC700_002868"
     CDS             51..1109
                     /locus_tag="PC700_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HDF2582147.1"
                     /translation="MNCKWISKIDERKKCHREADSSGYCIFHKENKSDEEIQLMMDTI
                     HKEEISEFNGFVFENEFNAEEILTYNYKILDFSESIFKQKANFKKYIFKKNIIFNYTE
                     FRDKVLFNGCIFLENCDFNRTIFSKNYINDRIFEKVKFKGPDLVVNKVENFPRMDGII
                     FSMCTKFVLKNVEYGKSEYEHGKINYRIARNQATKIGEYEMIGFYYYKERIYSSKIMK
                     RSNYPTFSDYLVEKFFDQIARYTTGYGEKPWNILLVIIAIISVFALLYLFVGIESSNS
                     TLVALDINNIGDYSLSEIFKMYMDLWYFSMATFSTVGYGDMVATSLIGKALAGIEVFF
                     GVTIGAIWASVIIKRMIR"
     gene            1213..2397
                     /locus_tag="PC700_002869"
     CDS             1213..2397
                     /locus_tag="PC700_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDF2582148.1"
                     /translation="MKNALKKIISLAMILVFIIPNYSYADGKSVDKYSKTSILIDQET
                     GRVLYSKEPDMKVPLASLSKMMTFLLAIEAIENKEVKETDKVKIDKSIASVKGSSYKL
                     KDGEEVPLIELMRGLMIVSGNDAAIAIAKHICKTKEAFVDRMNKKAKEIGMSNTHFLN
                     PNGLPIYDLSNPKKPAKENISTAKDIAILGKYMFDHYEKQVTAITDMETYTNSDRSFE
                     KSNTNALLRIIPEVDGIKTGYTGNAGYCLSFSMVVNKNEKNEKKRRVIGVVLGTNHKN
                     KRTSASLALLKYGKENFNMKKVIDKDSFIGKKYIKGMKELEVTLKAKDELYTILKDDE
                     SIKSEVKIKNIEYPVKKGDTMGVIKYYTSSGDLLGSVDIVSNNSIDNVSLKTKLKMKF
                     AN"
     gene            2604..3317
                     /gene="rpsB"
                     /locus_tag="PC700_002870"
     CDS             2604..3317
                     /gene="rpsB"
                     /locus_tag="PC700_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDF2582149.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIYIIDLQ
                     KTVKKVEEAYKFTKEVAETGKPILFVGTKKQAQDAIKDEAERCGMYFVNERWLGGMLT
                     NHKTIKTRINKLRELEKMEEEGVFNVLPKKEVIKLRAEKEKLEKYLGGIKDMPELPGA
                     MFVVDPRKENIAIQEAHRLGIPVVGIVDTNCDPEQLDFAIPGNDDAIRAVKLITGAMA
                     TAVIEGRQGAEEEVAEDQE"
     gene            3396..4307
                     /locus_tag="PC700_002871"
     CDS             3396..4307
                     /locus_tag="PC700_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDF2582150.1"
                     /translation="MANITAQMVKELRESTGAGMMDCKKALQEAEGNMEKAVDLLREK
                     GLSKAAKKAGRVAAEGLVAIEMNDDNTVASMVEVNSETDFVAKNEDFKVFVKDAACMA
                     LATDKEDIASLLGETHKEGITLQEVLNNRVAKIGEKLDFRRFAKVVTNGQVAGYIHGG
                     GKIGVLVEMETEARDAKVLELGKDVAMQVAAMNPKYVSRDEVDAEYIAHETEVLTQQA
                     LNEGKPANIVEKMVKGRLEKELKEVCLLEQTFVKNPDITVKQLVADVAKAVGSDIKVV
                     KVVRFEVGEGIQKREENFAEEVAKQLK"
     gene            4392..5096
                     /locus_tag="PC700_002872"
     CDS             4392..5096
                     /locus_tag="PC700_002872"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDF2582151.1"
                     /translation="MDKPMYKRVLLKLSGEALAGERGFGINNDVVNDIAIAIKKIQEI
                     GVEVAVVVGGGNFWRGRTSEGMDRTTADYIGMLATVMNAMALQDALENIDVATRVQTA
                     IDMRQIAEPYIRRRAVRHLEKERVVIFGAGTGNPYFTTDTTAALRAAEMEAEVILLAK
                     NVDAVYDKDPKVHADAKKFTELSYMEVIQKELKVMDSTATSLCMDNKIPIKVFELTTE
                     NIIRAVKGENIGTTVK"
     gene            5122..5679
                     /gene="frr"
                     /locus_tag="PC700_002873"
     CDS             5122..5679
                     /gene="frr"
                     /locus_tag="PC700_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDF2582152.1"
                     /translation="MKLEIHKQLEEKMNGTIDALKFEFGTIRAGRANAQMLDKIRVDY
                     YGTPTPINQIGAISVPEPRILMISPWDKSAMHEIEKAIANSDLGLNPSNDGEVIRLSV
                     PALTEERRKELAKKASKAAEEFKVRIRNERRDANEKIKKMEKGGELTEDELKKAQDEV
                     QKMTDKFIKEIDTLLSKKEKDIMEV"
     gene            5682..5885
                     /locus_tag="PC700_002874"
     CDS             5682..5885
                     /locus_tag="PC700_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582153.1"
                     /translation="MKKYYNLLGLHVDEVKQYFDEQNINYTVKSIEGKKDKEKLVVPR
                     VIKISEIGDSVELIITYFSDSLV"
     gene            5914..6648
                     /locus_tag="PC700_002875"
     CDS             5914..6648
                     /locus_tag="PC700_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HDF2582154.1"
                     /translation="MNNNIIYDIDLNNIPTHIAIIMDGNGRWAKARFLPRTAGHKAGV
                     ETIRDIVKECSKLGVKHLTLYAFSTENWKRPKLEVDTLMNLLSTYLRNEIAELHQNNV
                     KVTAIGDISALPKTCIRELNSAKELTKNNTGVNLNLALNYGSRADIKNALIDIVKNCE
                     SGKIDINNIDEDIIKNYLSTKSIPDPDLVIRTSGEQRLSNFLLWEVAYSEFYFTDIHW
                     PDFKKEELQKAIYVYQRRDRRFGGLK"
     gene            6661..7440
                     /locus_tag="PC700_002876"
     CDS             6661..7440
                     /locus_tag="PC700_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDF2582155.1"
                     /translation="MLTRIIASLALVPLFLFVVYGGIPLYIAETAIVYIALHEFYKAF
                     KIKDVHPIFIIGYLFSIYLAVKNIFNLPLEYTYAVIFILFLASIIYMLMGKNNVIDVS
                     ITFLGVFYIGVFLDFIIITINGFEKGSIYVWLIFVISFMTDIFAYFSGYLLGKHKLIP
                     KVSPKKTIEGAIGGIIGSTLCCILFGYLFGIDLLQLAIIGSIGSVIAQLGDLFASSIK
                     RYVGIKDYGKIIPGHGGILDRFDSVILVAPFVYSAIKFFIR"
BASE COUNT         3135 a          783 c         1455 g         2234 t
ORIGIN      
        1 aaactaatac aaaatagtaa taattgtttt gtactggagg atatatattt atgaattgta
       61 aatggatatc taagatagat gagagaaaaa aatgtcatag agaagcagat tcttctggat
      121 attgtatctt tcataaagaa aataagtctg atgaagagat acagttgatg atggatacta
      181 ttcataaaga agagataagt gaatttaatg gatttgtttt tgaaaatgaa tttaatgcgg
      241 aagaaatatt aacttacaat tataaaatac tggatttttc tgaatccata tttaaacaaa
      301 aagcaaattt taaaaaatat atttttaaaa agaatattat ttttaactat acagaattta
      361 gagataaagt tttatttaat ggttgtatat tcttagaaaa ctgtgatttt aatagaacca
      421 tatttagtaa aaactatata aatgaccgta tatttgaaaa agtgaaattt aaaggacctg
      481 atttggtagt taataaagta gaaaattttc cacgaatgga tggaataatt tttagcatgt
      541 gtacaaaatt tgtgttaaag aatgttgaat atggaaaaag tgagtatgag catggcaaga
      601 taaattatag aatagctaga aatcaagcaa caaaaattgg agaatatgag atgataggat
      661 tttactatta taaggaaaga atttatagta gtaaaataat gaaacgttct aattatccta
      721 ctttcagtga ttatttagtt gaaaaatttt ttgaccaaat agcacgttac acaacaggtt
      781 atggagaaaa gccatggaat atattattag tgattatagc aataataagt gtttttgctt
      841 tattatactt atttgtaggc atagaaagtt caaatagcac acttgtagct ttagatataa
      901 acaatatagg tgattactca ctaagtgaga ttttcaaaat gtatatggat ttatggtatt
      961 ttagcatggc tacatttagt acagtaggat atggagatat ggttgctaca agtttgattg
     1021 ggaaagcatt ggcaggtata gaagtatttt ttggagttac aataggagca atttgggcat
     1081 ctgtgattat taaaagaatg ataaggtaat atttgtgtaa atatggatag gtattaatta
     1141 ctagatataa attggaccaa atagggaaaa tttaaatata aatatactta ataatccaag
     1201 aaggtgaaaa atttgaaaaa tgcactaaaa aaaattatat cattagccat gattctagtt
     1261 tttataatac caaattattc atatgcagat ggtaaatctg tagacaagta ttcaaaaaca
     1321 tctatactta tagaccaaga aacaggtaga gttttatata gtaaagaacc tgatatgaag
     1381 gtaccattag ctagtttaag taagatgatg acttttttac ttgcaataga agctattgaa
     1441 aataaagaag taaaagaaac tgataaggta aaaatagaca aatctattgc atcagtaaaa
     1501 gggtcaagtt ataaacttaa agatggtgaa gaagtgccac ttatagagct tatgagaggt
     1561 cttatgattg tatcaggaaa tgatgcggca attgctatag caaaacatat ttgtaaaacg
     1621 aaagaagcat ttgtagatag aatgaataaa aaagcaaaag aaataggaat gagtaataca
     1681 cattttctaa atccaaatgg tcttccaata tacgatttaa gcaaccccaa aaaaccagca
     1741 aaagaaaata tctctactgc aaaagatata gctattcttg gaaaatacat gtttgaccat
     1801 tatgaaaaac aagtaacagc tataactgat atggagacat atacaaattc agatagaagt
     1861 tttgaaaaaa gcaatacaaa tgcattatta agaattatac cagaagtaga tggtataaaa
     1921 actggatata ctggcaatgc tggatattgc ttatcatttt ctatggttgt aaataaaaat
     1981 gagaaaaatg agaaaaaacg tagagtaata ggggttgtat taggtactaa ccataaaaat
     2041 aaaagaacct ctgcttcatt agctctactg aaatatggaa aagaaaattt caatatgaaa
     2101 aaagtaattg ataaagatag ctttattggt aagaaatata taaaaggaat gaaagagtta
     2161 gaggttacat taaaagcaaa agatgaactt tatacaatat taaaagatga tgaaagtata
     2221 aaatcagaag ttaagattaa aaatatagag taccctgtta aaaaaggtga tactatgggt
     2281 gttataaaat attatactag ttcaggtgac ttacttggaa gtgtagatat agtaagtaat
     2341 aatagtatag ataacgtatc attaaaaaca aaattaaaaa tgaaatttgc aaattaaatt
     2401 caaaaaatgg tataaaatac acaaaaaaat ataaaaatag aaaatatgga aaaaatattg
     2461 ttatttcttc gcctctgtga tatactacta gaggtgaaaa aataaaataa atcacacaca
     2521 gcattgggat ccttaaaagg tgcctattac atggtttttt agggagaaga tcaagctgga
     2581 ggtataaaac caggaggtaa gatatgtcag taatatcaat gaaacaatta ttagaagcag
     2641 gtgttcactt tggtcaccaa acaagaagat ggaaccctaa aatggctaag tatatattca
     2701 cagagagaaa tggaatatat ataatcgact tacaaaaaac agttaaaaaa gttgaagaag
     2761 cttacaagtt cactaaagaa gtagctgaaa caggaaagcc aatcttattc gtaggaacta
     2821 aaaaacaagc tcaagatgcg ataaaagatg aagctgaaag atgtggaatg tactttgtta
     2881 atgaaagatg gttaggtgga atgttaacaa accacaaaac tataaaaaca agaataaata
     2941 aattaagaga attagaaaaa atggaagaag aaggcgtatt taatgttctt cctaaaaaag
     3001 aagttataaa attaagagct gaaaaagaaa aactagaaaa atacttaggt ggaataaaag
     3061 acatgcctga attaccaggt gcaatgttcg tagttgaccc aagaaaagaa aacatagcta
     3121 ttcaagaagc tcatagatta ggtataccag tagttggtat agttgatact aactgtgacc
     3181 cagaacaatt agactttgct ataccaggaa atgatgacgc tataagagcc gtaaaattaa
     3241 taactggtgc tatggcaaca gctgtaattg aaggtagaca aggtgctgag gaagaagtag
     3301 ctgaagacca agaataataa tttgacaatt aaatggtaag ggtcgccctt accatttata
     3361 atatggaata gaataagtag gaggaaactt aaaaaatggc taatataact gctcaaatgg
     3421 taaaagagtt aagagaaagt acaggcgcag gaatgatgga ctgcaagaaa gctttacaag
     3481 aagctgaagg aaacatggaa aaagcagtag atttattaag agaaaaagga ttatcaaaag
     3541 ctgctaaaaa agctggtaga gttgctgctg aaggtctagt tgcaatagaa atgaatgatg
     3601 ataatacagt tgcttctatg gtagaagtta actcagagac agatttcgtt gctaaaaacg
     3661 aagacttcaa agtgttcgtt aaagatgctg cttgtatggc attagctact gataaagaag
     3721 atatagcttc tttattaggt gaaactcata aagagggaat aactttacaa gaagttttaa
     3781 ataatagagt tgcaaaaata ggtgagaaat tagactttag aagatttgct aaagttgtaa
     3841 ctaatggaca agttgctgga tacattcacg gtggtggaaa aataggtgtt cttgttgaaa
     3901 tggaaacaga agctagagat gctaaagttt tagaattagg taaagatgta gctatgcaag
     3961 ttgctgctat gaatcctaag tatgtttcaa gagatgaagt agatgctgaa tacatagcac
     4021 atgaaacaga agtattaact caacaagctt taaatgaagg aaaaccagct aatatagttg
     4081 aaaaaatggt taaaggaaga ttagaaaaag aattaaaaga ggtttgttta ttagagcaaa
     4141 ctttcgttaa aaatccagat ataactgtta aacaattagt tgctgatgtt gctaaggcag
     4201 taggttctga tataaaagtt gttaaagttg tgagattcga agttggtgaa ggtatacaaa
     4261 agagagaaga aaactttgca gaagaagttg ctaagcaact taagtaataa ccaaagagag
     4321 aacacgcagg tgttctcttt tttaaaaaat agaaaaaatt ggatatgaag gattgaaata
     4381 ggaggtttct gatggataaa ccaatgtaca aaagggtatt gttaaaatta agtggagaag
     4441 cattagcagg agaaagaggt tttggtataa ataatgatgt agttaatgat attgctattg
     4501 ctataaaaaa aatacaagaa ataggtgttg aagtagctgt agtagtaggt ggaggaaact
     4561 tctggagagg cagaaccagt gaaggaatgg atagaactac tgctgactat ataggaatgt
     4621 tagctacagt tatgaatgca atggcgctac aggatgcact agaaaatata gatgttgcta
     4681 caagagttca aactgctata gacatgagac aaattgcaga accatatata agaagaagag
     4741 cagttagaca cttagaaaag gaaagagttg ttatatttgg tgcaggaaca ggaaaccctt
     4801 actttacaac agatacaact gctgctttac gtgctgctga gatggaagca gaagttatat
     4861 tgctagctaa aaatgttgat gcagtatatg acaaggatcc aaaagtacat gcagatgcta
     4921 agaaatttac agaattaagt tacatggaag ttatacagaa agaattaaaa gtaatggatt
     4981 ctacagctac atctttatgt atggataata aaattccaat aaaggtattt gagcttacaa
     5041 cagagaatat aataagagca gtaaaaggcg aaaatatagg aactactgta aaataatgaa
     5101 gaatgaagga ggagtaaaat tatgaaactg gaaatacata agcaattaga agagaaaatg
     5161 aatgggacaa ttgatgcttt aaagtttgaa tttggaacta taagagcagg aagagctaat
     5221 gcacaaatgc tagacaaaat aagagttgat tattatggaa ctccaactcc aataaatcaa
     5281 ataggtgcta tatctgtacc agaaccaaga atattaatga tatctccttg ggataaatct
     5341 gctatgcatg aaatagaaaa agcgatagct aactcagact taggattaaa tccatcaaac
     5401 gatggtgagg ttataagact ttctgtacct gctttaacag aagaaagaag aaaagaatta
     5461 gctaaaaaag ctagtaaagc tgcagaagaa tttaaagtta gaataagaaa tgaaagaaga
     5521 gacgccaatg agaaaattaa aaagatggaa aaaggcggag aattaactga agatgaatta
     5581 aaaaaagcac aagatgaagt tcaaaaaatg actgataaat ttataaaaga aatagataca
     5641 ttattatcta aaaaagaaaa ggacataatg gaggtataat attgaaaaaa tattataacc
     5701 ttttaggtct tcatgtagat gaggtaaaac aatattttga tgagcaaaat ataaattata
     5761 ccgtaaaatc tattgaaggt aaaaaggata aagaaaaact tgtagttcct agagttataa
     5821 aaatatctga gataggtgat agtgtagaac tgataatcac atacttttct gactccttag
     5881 tttaaggagt cagaacaata ttggaggcaa aatatgaata acaacattat atatgatata
     5941 gacttaaata acatacctac tcatattgct attataatgg atggaaatgg aagatgggca
     6001 aaagctagat ttcttccaag aactgcagga cataaggctg gagtagaaac tataagagat
     6061 atagtaaaag aatgctcaaa attaggtgtt aagcacttaa cattatatgc attttcaaca
     6121 gaaaactgga aaagaccgaa acttgaagtt gacacactta tgaacttatt atctacatac
     6181 ttaagaaatg aaatagctga acttcaccaa aacaatgtaa aagtaactgc tataggagat
     6241 ataagtgctt taccaaaaac ttgtataaga gaattaaact cagctaagga actgacaaaa
     6301 aacaatacag gagttaactt aaatttagca ttaaactacg gaagtagagc agatataaaa
     6361 aatgctctta tagatatagt aaaaaattgt gaaagtggta aaatagatat aaacaatatt
     6421 gatgaagata ttataaaaaa ctatttaagc acaaagtcta taccagatcc agacttggta
     6481 ataagaacaa gtggtgaaca aagattgagt aactttttac tttgggaagt tgcttattct
     6541 gagttttact ttactgatat acattggcct gattttaaaa aagaagaact acagaaggct
     6601 atatacgtat atcaaagaag ggatagaaga tttggaggac ttaagtaagg agattatttt
     6661 atgcttacaa gaattattgc ttcactagct ctggttccat tatttctatt tgttgtatat
     6721 ggaggtattc ctctatatat agctgagaca gcaatagttt atattgcatt gcatgagttt
     6781 tataaggcat ttaaaataaa agatgtacat ccaatattca ttataggata tttattttct
     6841 atttatttag ctgtaaaaaa tatttttaat ttaccattag aatatactta tgcagtaatt
     6901 tttattttat ttttagcaag tataatttat atgttgatgg gcaaaaacaa tgttatagat
     6961 gtttcgatta cgtttttagg agttttttat ataggagtat ttttagattt tataattata
     7021 actataaatg gatttgaaaa aggtagtata tatgtatggc ttatatttgt gatatctttt
     7081 atgacagata tatttgcata ttttagtggt tatttacttg gaaaacataa gctaattcct
     7141 aaagttagtc ctaaaaagac tatagaagga gctattggag gaataatagg aagtacactt
     7201 tgttgtattt tatttggata tttatttggc attgatttgc ttcaattagc gataatagga
     7261 agtataggaa gtgtaatagc tcaattagga gatttatttg catcttctat aaagagatat
     7321 gtagggataa aggattatgg aaagataata cctggtcatg ggggtatatt agatagattt
     7381 gatagtgtta tattagttgc accttttgta tatagtgcga ttaaattttt tattagataa
     7441 agattggttg gatttaaaag agcaatctat aattgctagt aatgaaaata gctatgtaac
     7501 ttttaaatgc agagatattc tgcagttatt agttacatgg ctattttcat tgttcaaaaa
     7561 tttagaagac cttggggaat tttataagaa taagtattac tttataa
//
LOCUS       DAMJWP010000175         7505 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.182, whole genome shotgun sequence.
ACCESSION   DAMJWP010000175 DAMJWP010000000
VERSION     DAMJWP010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7505
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.182"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            169..1494
                     /gene="cdeA"
                     /locus_tag="PC700_002877"
     CDS             169..1494
                     /gene="cdeA"
                     /locus_tag="PC700_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter CdeA"
                     /protein_id="HDF2582156.1"
                     /translation="MENLFTRKFTTFEFLKFVSPAIISMIFISLYTIIDGIFVSTLVG
                     SDALASINIVLPIINLVCGFGIMMATGGGAIVSIRMGENRQDEANSTFSFIVLFSLIV
                     GISFTVISYFFIKEISILLGATDKLLPYCITYGKVMILCTPFYILKFIFNYFARTDGN
                     SKFSLFLSVIGGVTNIILDYVFIKYFGMGLLGAAVATAIGIILTCVLGIIYFLSNKST
                     LKLRKPKTDFRLIRDTMINGSSEMVTELSTGITTFLFNVVALKLAGENGLAALTIVLY
                     AHFLMTSVYLGFAAGVSPLISYNFGAENSDKLKETFKHSLKFIFVSSLLVFIIALVFA
                     PFIVRVFVSPDNTVFKLALQGLKIFAFAFLFVGINIFASGFFTAFHNGKISAIISFSR
                     AFVFIIIGIIILPPMLNMTGLWLTVPFAEVITIFISILFIKKYKGRYKY"
     gene            complement(1589..2383)
                     /locus_tag="PC700_002878"
     CDS             complement(1589..2383)
                     /locus_tag="PC700_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2582157.1"
                     /translation="MNELKPYIKFFNKNTKLYIIIFMIVGIVMNILPVFILKIFGEAS
                     KTNLSTIVNPILTYITILLFVYGVNIANKDFSGALSIRADRKSCIKAIVIDLIILSFV
                     ISILGIVLIFLSKLFIEIITGYSIELSVLLKRDMIWTSISALIDPSTSSMPLTYLNYF
                     VQICINEICIGFIGVLLGAFTYRVRKVTSITILIGLPILMVIYIVNFATKNMHKMLLY
                     LEKIIYSFQNPFILFGTKVGIIVICIIFIILLLRKAPIKEYANDLL"
     gene            complement(2384..3259)
                     /locus_tag="PC700_002879"
     CDS             complement(2384..3259)
                     /locus_tag="PC700_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2582158.1"
                     /translation="MRSSIKIENLSHSFKKQNIFNNMSLNFEKNKIYGLLGKNGAGKT
                     TLLNIMVNQLIQNEGEIEILGKNPKEDIKVLEEVCIVREKEFYSPDFKVGQIFEFSSS
                     FYKSYDKGLEEELCKYFDLNTKKKYKQLSRGMKTILSNIIGICSNSAITIFDEPTIGL
                     DAVNRQEFYNIILDSYIKNPRTIIISTHLIDEIDDLLEHVIILNEGKIIIDEEIDVIK
                     QKAHYITGNKEELEKLECIKNIKPKKAFGNAVAYFYYGDFSENDEKILKNSNIDVGYI
                     GLQDMFVNMTKKEEL"
     gene            complement(3263..3631)
                     /locus_tag="PC700_002880"
     CDS             complement(3263..3631)
                     /locus_tag="PC700_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2582159.1"
                     /translation="MKLVLNNEEPIFIQIARAIEDEILSNGIKEEEQVPSTTELSKLY
                     KINPATVLKGINILVDKNILYKKRGIGMFVSDGAKTIIKEARKENFKHNFVKNLLQEA
                     NKLEINREELVDIIINFKED"
     gene            complement(3814..4473)
                     /gene="deoC"
                     /locus_tag="PC700_002881"
     CDS             complement(3814..4473)
                     /gene="deoC"
                     /locus_tag="PC700_002881"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDF2582160.1"
                     /translation="MKHILKTVDHTILKATTTWEDIKILCDEAVDMSVASVCIPPSYV
                     KRASEYLKGKIKICTVIGFPLGYQTTATKVFEAKDAIENGADEVDMVVNISDIKNKDY
                     DNIGKEIKEIKKAIGDKVLKVIIETCYLDEDEKIKMCEIVTMSGSDFIKTSTGMGTGG
                     ATLEDIKLMKEHVGKNVKIKAAGGVKSISDAEKFIEAGAERLGTSSICKILKNEDTTD
                     Y"
     gene            complement(4509..6053)
                     /gene="murJ"
                     /locus_tag="PC700_002882"
     CDS             complement(4509..6053)
                     /gene="murJ"
                     /locus_tag="PC700_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HDF2582161.1"
                     /translation="MSKVAKATFYLMIVTIISKILGMGRELVLSSIYGTGLYTESYLT
                     AMNIPNIIFAAIGTAIVTTFIPMYQDISSKQGEKQALKFLNNVLNIIVGICIVVAILG
                     VIFSKQLVSIFAIGFEGERFLLTVKFTKILITGIIFIGITSVMSAFLQIKENFIVVGF
                     GSIPYNIVIIISIMLSTVFGPYILPIGAVVAMVVQLLFYMFFVKKTNYKYLYYLNFKD
                     DSLIKLLALLSPVFIGVAVNQVNSLVDTTLASTLVKGSIPALTYSDRLNGFVTGTFTA
                     SIVSVMYPMLSKLSAENNQKKFTSSVKSSINMIIISMIPISVASIFFATPVVRIIFER
                     GAFDARATQMTATALIFYAVGMTAFGLRDILGKVFYSLQDTKTPMVNGIISVGVNIVL
                     DLVLIKPMAHGGLALATSSSSIACILLLFLNLKRKVGYFGQDKIIKATLKSVVASLIM
                     GVLSYFTYKFIFGILGVGTFNEFVSLAISVIVGGGIYTLLMTIFKVEEVDMILNIAKR
                     KLHLKK"
     gene            complement(6527..7411)
                     /locus_tag="PC700_002883"
     CDS             complement(6527..7411)
                     /locus_tag="PC700_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2582162.1"
                     /translation="MDTKKENNYENYICVGSSNVSNAMKMYLKEIEEYKMLSAGEEVE
                     LAKEIINSSSVAKEKFINSNYRLVVSIAKRYKRDSIDMLDLIQAGNIGLIKAVEKYDY
                     KKGYKFSTYATWWIKQSITRYIDDCENTIRIPIHLHQRINFVKKKKQELLNVLLREPT
                     IDEIADACGLEVDKVLELLRRDKNVVSLDTPLKEDEDSSLVEFIPSDADFKDVVIHEV
                     EQHNLKEKIEELLTGLGEQEQQVLRMRFGIDDDDPKTLEQIGKVFGVTRERIRQIEAK
                     AIRKLRHPSKLKQLKHFY"
BASE COUNT         2495 a         1219 c          790 g         3001 t
ORIGIN      
        1 tatacctatt tttactaata taagtttatt aattagcatt atactaattt atcttaatat
       61 gattttttgt tgattttaag ctgtttatcg tataaataaa atttttcttg acatatagtt
      121 actatatagc ttattataga tagagagtaa taaaaggagg taaaatttat ggaaaattta
      181 tttacaagaa aattcactac ttttgaattt ctaaaatttg tttctcctgc aattatatcc
      241 atgatattta tatctttgta cacaataata gatggcatct ttgtatcaac attagttggt
      301 tccgatgctc ttgctagtat aaatattgta ctacctataa ttaaccttgt ttgtggattt
      361 ggaataatga tggcaacggg tggaggtgct atcgtttcta tacgtatggg tgaaaataga
      421 caggatgaag ccaactctac attttctttt atagttttgt tttcattgat tgttgggatt
      481 tcattcacag taatctcata tttctttatc aaagaaatat ctattttgct tggtgcaaca
      541 gataagttat taccatattg tataacttat ggtaaagtta tgattttatg tacaccattt
      601 tatattttaa aatttatatt taactacttt gcaagaactg atggaaattc taaatttagt
      661 ttatttctat cagtcattgg tggtgtaaca aatataatat tggattatgt atttattaaa
      721 tattttggaa tgggtctttt aggagctgca gttgcaactg ctataggtat tattttaact
      781 tgtgttttag gtattattta cttcttatct aataaatcta cactcaaatt aagaaaacca
      841 aaaaccgatt ttagacttat aagagatact atgataaacg gttcttctga aatggttaca
      901 gaattatcta caggaattac aacattctta tttaatgtag tagctttaaa attagcagga
      961 gaaaatggac ttgctgctct tactatagta ttgtatgctc attttttaat gacatcagtc
     1021 tatctaggat tcgctgctgg agtgtctcca ttaataagct ataattttgg tgctgaaaac
     1081 agtgataaat taaaagaaac atttaaacat tctctaaaat ttatatttgt ttcttctctt
     1141 ttagtgttta ttattgcttt agtatttgca ccatttattg ttagggtttt tgtaagtcca
     1201 gataacacag tatttaaact agccttacaa ggattaaaaa tatttgcatt tgcttttttg
     1261 tttgttggta taaatatatt tgcatcagga ttttttacag catttcacaa tggaaaaatt
     1321 tcagctatta tatcttttag tcgtgccttt gtttttataa tcataggaat cataattctt
     1381 cctcctatgt taaacatgac tggattatgg cttacagttc catttgctga ggttataacc
     1441 atatttatat ctattctatt tataaaaaaa tataaaggta gatataagta ttaaaaatta
     1501 atgcttaaaa tcaacattta ttaaaactaa taaacctgct ttaaaataca aatttacaaa
     1561 tttgattttt aagcaggttt tattttagtt ataataagtc atttgcatac tctttaattg
     1621 gtgcttttct aagtaataaa attataaata ttatgcaaat aactataatt cctacctttg
     1681 ttccaaataa aataaaagga ttctgaaatg aataaataat tttttctaaa tacaatagca
     1741 ttttatgcat atttttagtt gcaaaattaa ctatataaat taccattaat atcggtaatc
     1801 ctatcaatat agtaatacta gtaacttttc taactctata tgtaaaagct ccaagtaata
     1861 ctcctataaa acctatacat atttcattta tacatatttg tacaaaataa tttaaataag
     1921 ttagtggcat acttgatgtt gaaggatcta tcagtgctga tatagatgtc caaatcatat
     1981 ctctctttag taaaactgat agttctatac tgtaacctgt tataatttct ataaataact
     2041 tcgataaaaa aatcaaaact atacctaaaa tagaaatcac aaagcttaaa ataattaaat
     2101 ctatgacaat agcttttata caactttttc gatctgctct tatacttaaa gctcctgaaa
     2161 agtctttatt tgctatattt actccataca caaatagcag tatagttata tatgttaaaa
     2221 taggattcac aatcgtggat aaattagttt tagatgcttc tccaaaaatc ttgaggataa
     2281 acactggcaa aatattcata acaattccca caatcataaa tataattata tacaatttag
     2341 tatttttatt aaaaaatttt atatatggct ttaattcatt caattacaat tcctccttct
     2401 tagtcatgtt aacaaacata tcttgtagcc ctatgtatcc tacatcaata tttgaatttt
     2461 taagtatttt ttcatcattt tcactaaaat caccatagta aaaataagca actgcatttc
     2521 caaatgcctt tttaggtttt atatttttta tacattctaa tttttctaac tcttctttat
     2581 ttccagtaat ataatgagct ttttgtttta tgacatcaat ttcttcatct ataataattt
     2641 ttccctcatt taatattatt acatgctcca gtaaatcatc tatttcatca atcaaatgag
     2701 ttgatattat tattgtcctt ggatttttta tataactatc taaaattatg ttataaaact
     2761 cttgtctatt tactgcatcc aaaccaattg ttggctcatc aaatatggtg atagctgaat
     2821 tagaacaaat acctattata tttgaaagta tagttttcat acctcttgac agttgtttat
     2881 actttttttt agtatttaaa tcaaaatatt tgcataattc ttcttctaag cctttatcat
     2941 aacttttata aaaactagaa gaaaattcaa atatctgacc tactttaaaa tctggactat
     3001 aaaattcttt ttccctgacg atacaaactt cttccaaaac ttttatatct tcttttgggt
     3061 tctttcctaa tatttctatc tcaccttcat tttgaattaa ctgatttacc attatattta
     3121 ataatgttgt ttttccagct ccattcttac ctaataaacc atatatctta tttttttcaa
     3181 agtttagaga catattatta aaaatatttt gcttcttaaa actgtgagat aaattttcta
     3241 tcttaataga actcctcata tattaatcct ccttgaaatt aattataata tcaaccaatt
     3301 cttctctatt tatttctaat ttgttagctt cttgaagtag gttttttaca aaattatgtt
     3361 taaaattttc ttttctagct tcttttataa tagtttttgc accatctgat acaaacatcc
     3421 ctatccccct cttcttatat aaaatatttt tatcaaccaa tatatttata ccttttaata
     3481 ctgtagctgg attaatttta taaagctttg aaagctctgt tgttgatgga acctgttctt
     3541 cttcttttat tccatttgat agtatctcat cttcaattgc tcgtgctatc tgaataaata
     3601 taggttcttc attatttaat actaatttca taggttcctc ctttctccat tagttagtta
     3661 ctcaactaat taactatata acttataata tcttgaaaaa tattttctgt caatactaaa
     3721 taaaaaacag agggaaaatt ccctctgttt tttgttcttt atatttataa ttaaattttg
     3781 atttttgtaa tcctatccca taagttaaaa actttaataa tctgttgtat cttcattttt
     3841 taatatttta catattgagc tagttcctaa cctttctgct cctgcctcta taaatttttc
     3901 tgcatcagaa attgatttaa ctccccctgc agcttttatt tttacatttt ttccaacatg
     3961 ctctttcata agctttatgt cttctaaagt agctccacct gttcccattc cagtagatgt
     4021 ttttataaaa tctgaacctg acattgttac tatttcacac atttttattt tttcatcttc
     4081 atctaaataa catgtctcta taatgacttt taatacttta tctcctatag ctttttttat
     4141 ttcttttatt tcttttccta tattatcata atctttattt tttatatctg aaatgtttac
     4201 aaccatatca acttcatctg ctccattttc aatagcatct tttgcttcaa atacttttgt
     4261 agcagtagtt tgatatccca aagggaaccc tatcactgta catatcttta ttttaccttt
     4321 tagatattct gatgctcttt tcacatatga tggtggtatg caaactgatg ctacactcat
     4381 atctacagct tcatcacata aaattttaat atcttcccat gtagttgtag cttttaaaat
     4441 agtatggtct actgttttta atatatgttt cataaaatcc tcctaaattt ataattaata
     4501 taattagatt acttttttaa atgcaatttt ctctttgcaa tattcagtat catatcaacc
     4561 tcttcaactt taaaaatagt cattagtagt gtataaattc cacctccaac tataactgaa
     4621 atagctagtg atacaaactc attaaatgtt cctactccca aaattccaaa tataaattta
     4681 tacgtaaagt atgataaaac acccataatt aaagatgcaa ctaccgattt caaagttgct
     4741 tttattattt tatcttgtcc aaaatatcca acttttctct ttaggttcaa gaagagtaac
     4801 aatatacatg caattgatga tgaactagtt gcaagagcaa gaccaccatg agccattggt
     4861 tttattaaaa ctaagtccaa tactatattt acccctacag atataatccc atttaccata
     4921 ggtgtttttg tgtcttgtaa tgaataaaaa acctttccca aaatatctct taatccaaat
     4981 gcagtcatac caactgcata aaaaattaag gcagttgctg tcatttgggt tgccctcgca
     5041 tcaaaagcac ctctttcaaa aatgattcta acaacaggag ttgcaaaaaa aatagaagca
     5101 actgaaatag gaatcataga aattattatc atatttatac tagatttaac tgaactagta
     5161 aacttctttt gattattttc tgcacttaac ttagataaca taggatacat aacagatact
     5221 attgaagctg taaatgtccc tgtaacaaaa ccatttaatc tatctgaata agtaagtgct
     5281 ggaatgcttc ctttaactaa tgttgaggct aaagttgtat ctaccaatga gttcacttga
     5341 ttaacagcaa cacctataaa tactggtgaa agtaatgcta gtaatttaat aaggctatca
     5401 tctttaaagt ttaagtagta taagtatttg tagttcgttt ttttaacaaa aaacatgtaa
     5461 aagagcagtt gaactaccat agcaaccaca gcaccaattg gaagtatgta tggtccaaat
     5521 acggtactta acattattga aataattatt actatattat agggtatact cccaaatcca
     5581 actacaataa agttctcttt aatttgcaga aatgctgaca taacactcgt aatacctatg
     5641 aatattattc ctgttatcaa tattttagta aattttacgg taagtaaaaa cctctctcct
     5701 tcaaatccta tagcaaatat acttacaagt tgctttgaga aaataactcc taatatagct
     5761 acaactatgc atataccaac tattatattt aaaacattgt ttaaaaactt tagtgcttgc
     5821 ttttcacctt gtttactact tatatcttga tacattggaa taaaagtagt aactatagca
     5881 gtacctattg ctgcaaatat aatatttggt atattcatag ctgttaaata actctctgta
     5941 tataagcctg taccataaat tgatgataat actaattctc tacccattcc aagaatctta
     6001 gagattatag taactatcat caaataaaaa gttgctttag ctaccttgct cattttatca
     6061 ctccacttct aacatacctc tttttaatca agtaaaattt ggctcattag atataattct
     6121 atcaactaat ttgccttgct tagccctatc aaaatataat taacacctat ttcttaatat
     6181 atcacaaact acattattct tcatattttt ttatttctcc aataagtttt acatctttcc
     6241 tatataaatt gtatagcttc aattatattc aaagaaatgg agttgctacc cgataacttt
     6301 aaaagtaaaa aacagatttt attatctttt taaactatat tctaacatat tttgtccata
     6361 ttgagaaatt ttactaaact attatgtact ttattaaata aataaaataa cagcacacct
     6421 ataaaataca agtgtgctgt tcataaacag attttataaa aaaataattc ctaagtagag
     6481 attaacatgc tttctcatac tctcatatat aatgtgtcta aaaatattaa taaaaatgct
     6541 ttaattgttt taacttactt ggatgtctta attttcttat tgctttagct tctatctgtc
     6601 ttattcgttc tctggtaact ccaaatacct ttccaatctg ttctagtgtt ttagggtcat
     6661 catcatcaat tccaaatctc attcttaaaa cttgctgttc ttgctcacca agacctgtaa
     6721 gtagttcttc tatcttttct tttaaattat gctgttcaac ttcgtgtatt acaacatctt
     6781 taaaatcagc atctgatggt ataaattcaa ctaaactact atcctcatct tcttttaatg
     6841 gagtatccaa agatacaaca tttttatctc ttcttaataa ttccaacact ttgtcaactt
     6901 ctaagccaca tgcatctgca atctcatcta tagttggctc tcttaataat acatttaaaa
     6961 gttcttgttt ctttttcttt acaaaattta ttctctgatg aagatgtata ggtatcctaa
     7021 tagtattttc gcaatcgtcg atatatcttg ttatactttg ttttatccac caagttgcat
     7081 aagtgctaaa tttgtatcct tttttataat cgtacttttc tacagctttt ataagtccta
     7141 tatttcctgc ttgaattaaa tccaacatat caatactatc tcttttatat ctctttgcta
     7201 tgctcactac caacctataa tttgaattta taaatttttc ttttgctact gaagatgagt
     7261 tgattatttc cttagccagc tctacctctt ctcctgctga caacattttg tattcctcta
     7321 tttcctttaa atacattttc atcgcatttg atacatttga tgaacccaca caaatataat
     7381 tttcataatt attttctttt ttagtatcca taatcaaacc ccttctattc atagaatttg
     7441 ctaaaacaaa aataaataat ttaaacctac ataatctaca attacataat atcccattct
     7501 ttaaa
//
LOCUS       DAMJWP010000176         7496 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.183, whole genome shotgun sequence.
ACCESSION   DAMJWP010000176 DAMJWP010000000
VERSION     DAMJWP010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7496
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.183"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            116..1093
                     /locus_tag="PC700_002884"
     CDS             116..1093
                     /locus_tag="PC700_002884"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDF2582163.1"
                     /translation="MRDKIIEIILNNKSEFISGEELSKQLGVSRAAIWKHMKALKEEG
                     YNIESVNKKGYRLAENPTDLLSPQNIYYKLDTEFIGKNIEHLDTIDSTNEHAKRIAIN
                     SVDGTVILSEHQDMGKGRLGRRWDSKTHEGIWMSIILKPDMEPFKAPFITLIAGASVT
                     KALNNLGANTLIKWPNDVILNGKKICGILTELSAEIERTNYVVLGIGINVKIMDFEQE
                     IADKATSLYKEGYMLSRVDIVRNVLTEFEKLYLDYTVNNSKEKTLKICRDYSAIIDKN
                     IYVLKGDNRELVKCLDINEEGNLLVQRSDGSIKEIISGEVSIRGEKGYV"
     gene            1086..2054
                     /locus_tag="PC700_002885"
     CDS             1086..2054
                     /locus_tag="PC700_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HDF2582164.1"
                     /translation="MYNNILDIEGLKVGQVEDRNGLTGCTVVICEEGATCGVDVRGSA
                     PGTRETDLLDPVNMVQKVHAVVLAGGSAYGLESTCGVSRYLEEKGIGFDVGVGKVPIV
                     VGAVLFDLGVGSFKCRPDLKMGYRACEVANTDILNQGNYGAGCGATVGKIRGQEYAMK
                     GGIGSYSIKLDNGLIVSALIAVNALGDVYENGKVIAGALNDKKTETIDTYKLMKQGES
                     KGGFSVDNTTIGIIATNAKLTKAGCKKISQMGHDGYAKSIFPIHTPHDGDTIFTISTG
                     EIEADITLVGSLAVEVVEKSIINAVKKADSVENIMAYKDIVKKREI"
     gene            2238..2828
                     /locus_tag="PC700_002886"
     CDS             2238..2828
                     /locus_tag="PC700_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF2582165.1"
                     /translation="MMNTSASVRKVNVRKMTIIGVLSAISIMLSMTPLGFIPVGPTKA
                     TIMHIPVIIGAIMEGPIVGAAIGFIFGISSLLNAITNPTVTSFVFINPLVSILPRVMI
                     GVLAYYVYQLIIKATNKVYISGLITGAIGSLLNTAGVLGMIYVLYADKYLHAMGKSGS
                     AGKVIMALAAANGVPEAIVGALVVAAVATVLKKSKK"
     gene            2847..3617
                     /locus_tag="PC700_002887"
     CDS             2847..3617
                     /locus_tag="PC700_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HDF2582166.1"
                     /translation="MLLVFDVGNTNMVLGIYKGDKLVNYWRIKTDREKTSDEYGILIS
                     NLFDYDNVNISDIDDVIISSVVPNVMHSLENFCIKYCKKQPLIVGPGIKTGLNIKYDN
                     PKQVGADRIVNAVAGIEKYGAPSILVDFGTATTFCAISEKGEYLGGTIAPGIKISSEA
                     LFQSASKLPRVELAKPGMTICKSTVSAMQSGIIYGYVGLVDKIISMMKKELNCDDVKV
                     IATGGLAKLIASETKSIDYVDGFLTLEGLRIIYEKNQE"
     gene            3754..4722
                     /gene="dusB"
                     /locus_tag="PC700_002888"
     CDS             3754..4722
                     /gene="dusB"
                     /locus_tag="PC700_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDF2582167.1"
                     /translation="MKIGNLELKNKVFLSPMAGVTDLPFRLICKEQGCGLLYTEMING
                     KALCYDDENTKKMLKIEEEEHPVAVQIFGSEPEFMGRAAEIMNDYSNEILDINMGCPA
                     PKVVKNGDGSALMKNPKLAEEVLRAVVKNSKKPVTLKIRKGWDDNSVNAVEIAKIAED
                     CGISALAIHGRTREQFYTGKADWDIIAEIKKNLSIPVIGNGDVFTIEDSINMLDKTGC
                     DAIMIGRGAQGNPWIFKRINHYMNTGEILPEPTLNEKISTAIKHLKLAVEEHGEYVAV
                     REMRKHIAWYLKGLRNSAKLRDEINKIEDYQEVVSKLEYYMEDSLT"
     gene            4831..5310
                     /gene="greA"
                     /locus_tag="PC700_002889"
     CDS             4831..5310
                     /gene="greA"
                     /locus_tag="PC700_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDF2582168.1"
                     /translation="MEENKEFLLTQEGYDKLEEELENLKVVKRKEVAERIKVAISFGD
                     LSENAEYDEAKKEQAQVEERILKLENMVRKAVIIDESKIDLNVVTIGSIVKVKDLEFD
                     EEVEYTIVGSTEADPYDGKISNESPVGKALLGRAAKEVVEVQVPDGVAKFEILEIRR"
     gene            5325..6854
                     /gene="lysS"
                     /locus_tag="PC700_002890"
     CDS             5325..6854
                     /gene="lysS"
                     /locus_tag="PC700_002890"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDF2582169.1"
                     /translation="MKNNQQSNEEAQIQEDLSEVLQVRRDKLKKLQESGRDPFKESRY
                     DRTHYSMDIKDNFDSLEGKTTKIAGRIMSKRIQGKAGFIDIQDQEGRIQSYVRLDAIG
                     EEEYSVFSTYDIGDIVGIEGEIFKTKKGEISVKAKSVVLLCKSLQVLPEKYHGLKDQE
                     LRYRQRYVDLIVNPEVKNAFLIRTKALKALRAYLDDRGFLEVETPILNTIAGGANARP
                     FITNHNTLHIPMYLRIANELYLKRLIVGGFDKVYEMGRMFRNEGMDLKHNPEYTAIEL
                     YQAYADYTDMMEITENVIAHMAEVATGSMIVNYQGTEINFTPPWKRMSMEDCVKEYSG
                     VDFSTINTDEEALEVAREKGIEIKPGMRRGEVINAFFEEFGEDKLIQPTFITHHPVEV
                     SPLSKRNVEDPRRTDRFEAFANKWELANAFSELNDPIDQKGRFIDQLRKRELGDDEAF
                     EMDEDFLKALEVGLPPTGGLGIGIDRVIMLLTNSPSIRDVLLFPTMKPIDNNPNKEEE
                     N"
     gene            7163..7357
                     /locus_tag="PC700_002891"
     CDS             7163..7357
                     /locus_tag="PC700_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582170.1"
                     /translation="MFQVINFLCLFCVGFLVLDVMIYFCKDAMDIFEGTTNVNKVKSI
                     NIWADRVRANSYSYENKIAK"
BASE COUNT         2995 a          753 c         1512 g         2236 t
ORIGIN      
        1 ttataataat taaaaaggaa atttaaaatt aatagtatta aaagtatatt aataatgtat
       61 aattatttat tagaagaaaa taagtcatga aattatgtca tattagaggt gaaatatgcg
      121 agataaaata atagagatta tattaaataa taaatctgaa tttatttcag gagaagaatt
      181 atcaaaacaa ttaggggtgt cacgtgcagc tatatggaaa catatgaaag ccttaaaaga
      241 agagggatat aatatagaat ctgtaaataa aaaagggtat cgattagcgg aaaatccaac
      301 tgatttatta agcccacaga atatttacta taaattagat acagagttta ttggaaaaaa
      361 tattgaacat cttgacacta tagattcaac aaatgaacat gctaaaagga tagctataaa
      421 tagtgtagat gggactgtga ttttaagtga acatcaagat atggggaaag gacgattggg
      481 aagaagatgg gattcaaaaa ctcatgaagg aatctggatg agtattatat taaaaccaga
      541 tatggaacca ttcaaagctc catttataac tttaatagca ggagcaagtg taacgaaagc
      601 tttaaacaat ctaggagcaa atacattaat aaaatggcca aatgatgtta ttttaaatgg
      661 aaaaaaaata tgtggtatat taacagagtt atctgctgaa attgaaagaa caaattatgt
      721 tgttcttgga attggaataa atgttaaaat aatggatttt gaacaagaaa tagctgacaa
      781 agccacatca ttgtataaag aagggtatat gttatccaga gtagatattg ttaggaatgt
      841 attgactgaa tttgaaaaat tatatttaga ttatactgta aataattcca aagaaaaaac
      901 cttaaaaata tgtagagatt attctgcaat tatagataaa aatatatatg tattaaaagg
      961 agataataga gaactagtga aatgtttaga cataaatgaa gagggaaatt tattagttca
     1021 aagaagtgat ggaagtataa aagaaattat ttcaggtgaa gtttctataa gaggagaaaa
     1081 aggatatgta taataatatt ttggatatag aaggcttaaa ggttgggcag gttgaggata
     1141 gaaatggtct tactgggtgc acagtagtaa tatgtgaaga aggagctact tgcggtgttg
     1201 atgtaagggg ttctgctcca gggactagag aaaccgattt attagaccct gtaaatatgg
     1261 ttcaaaaagt tcatgcagta gttttggctg gaggttctgc atatggtctt gaatccactt
     1321 gtggtgtaag tagatactta gaagaaaaag gaattggctt tgatgtaggt gtaggtaaag
     1381 tgcctatagt tgtaggagca gttttatttg atttaggagt agggagcttt aaatgtagac
     1441 cagatttaaa gatgggttat agagcttgtg aagttgcaaa tactgatatt ttaaatcaag
     1501 gaaattatgg agcaggttgt ggagccactg ttggaaaaat aagaggtcag gaatatgcta
     1561 tgaaaggcgg aataggtagc tattctataa aattagataa tggtctaatt gtatctgcat
     1621 taattgctgt aaatgctctt ggagatgttt atgaaaatgg taaggttata gctggtgctt
     1681 taaatgataa aaagacagaa acaattgata catataaatt aatgaaacaa ggtgaaagta
     1741 aaggtggatt tagtgtagat aatactacaa ttgggataat tgcaacaaat gctaagttaa
     1801 ccaaagctgg atgtaaaaag atatctcaaa tgggacatga tggatatgct aagtctattt
     1861 ttcctatcca tacacctcat gatggagata caatattcac aatatcaaca ggagaaatag
     1921 aagcagatat aacattagta ggttcattgg ctgtagaggt tgtggaaaaa agtataataa
     1981 atgcagtaaa aaaagcggat agtgtagaaa atataatggc atataaagat atagtaaaaa
     2041 aacgagaaat atgatgaaat tagcatattt ttcttgtcaa attgttgaca atggcaactc
     2101 atcttgataa aattattctt gttagagcgg gcatcctaca atggtagctt gactcatagt
     2161 tataagaaaa aaacgaaaaa aattaaaaaa ttaagtagtc cggcatctta atgagctgga
     2221 acggaggtgt attttttatg atgaacacaa gtgcatcagt aagaaaagta aatgtaagaa
     2281 agatgactat aataggagta ctatcagcta tttctattat gctatctatg actccattgg
     2341 gatttattcc agttggacct acaaaagcaa ctataatgca cataccagtt attattggag
     2401 ctattatgga aggaccaata gttggggcag caataggatt tatatttggg atatcaagtc
     2461 tcttaaatgc tataacaaac ccaacagtaa catcttttgt ttttataaat ccattagttt
     2521 caatattacc aagagttatg ataggtgtat tagcttatta tgtatatcag ttaataatta
     2581 aggctacaaa taaggtatac atatcaggtt taattacagg ggcaataggt tctctgttaa
     2641 atacagctgg agttttagga atgatatatg tcttatatgc tgacaagtat cttcatgcaa
     2701 tgggtaaaag tggttcagca ggaaaagtaa taatggctct tgccgcagca aatggggtgc
     2761 cagaagcaat agtaggggct ttagtagtag ctgctgttgc cactgtactt aagaaaagca
     2821 aaaaatagaa agaaggttaa tgacatatgc ttctagtatt tgatgttgga aatactaata
     2881 tggttttagg tatatataaa ggtgacaaat tagttaatta ctggagaatt aaaacagata
     2941 gggaaaaaac gtctgatgaa tatggaatcc tgataagtaa cctatttgat tatgataatg
     3001 tgaatataag tgatattgat gatgttataa tatcatctgt agttccaaat gtcatgcatt
     3061 ctcttgaaaa cttttgtata aagtactgta aaaaacagcc attaatagta ggtccaggca
     3121 taaaaacagg tctaaatata aaatatgata atccaaaaca agttggggca gatagaatag
     3181 ttaatgctgt agcagggata gaaaagtatg gagcaccaag tatacttgtt gattttggaa
     3241 cagcaactac attttgtgct atctctgaaa aaggtgaata tttgggtgga acaatagcac
     3301 caggaataaa aatatctagt gaggcgttat ttcaaagtgc gtctaaatta cctagagtag
     3361 aattagctaa gccaggtatg actatttgta agagtactgt atcagccatg caatctggaa
     3421 taatttatgg atatgttggt ttagttgaca aaataataag tatgatgaag aaagaattga
     3481 attgtgatga tgttaaggtt atagctacag gtggattagc taaactgatt gcttcagaga
     3541 cgaaaagtat agattatgta gatggttttt taacactaga aggattgaga ataatatatg
     3601 aaaaaaacca agaataatta atatacaggg aattgggtta tatatccaat tcccttttta
     3661 tgatagattt ctagctatat gtggtatatt ataatgagga aataagaatt taaagttaaa
     3721 tcacaaaata aaggcaaaaa taggagaaaa acaatgaaaa taggaaattt agaattgaaa
     3781 aataaagttt ttctatcacc tatggcaggt gtaacagacc ttccatttag attaatttgt
     3841 aaagaacagg gatgtgggct tttatataca gaaatgataa atggtaaggc actttgctat
     3901 gatgatgaaa atactaaaaa aatgcttaag atagaagagg aagaacatcc tgtagcagtt
     3961 cagatattcg gttcagaacc agagtttatg ggaagagcag cagagataat gaatgattat
     4021 tcaaatgaga ttctcgatat aaatatgggg tgtccagctc caaaagtagt taaaaatgga
     4081 gatggttcag cacttatgaa aaatcctaag cttgcagaag aggtattgag ggcagtagtg
     4141 aagaactcaa aaaagccagt gactttaaag attcgaaaag gctgggatga taatagtgta
     4201 aatgctgttg aaatagcaaa gatagcagag gattgtggaa taagcgcttt agcaatacat
     4261 ggaagaacta gagaacagtt ttatacaggc aaagcagact gggatataat tgcagagatt
     4321 aagaaaaatt tgagtatacc tgttatagga aatggtgatg tatttacaat agaagattca
     4381 ataaatatgt tagacaaaac tggttgtgat gctataatga taggtagggg tgctcaaggt
     4441 aacccttgga tatttaaaag aataaaccat tatatgaata caggcgagat tttgccagag
     4501 ccaactctta acgaaaaaat aagtactgcc attaagcatt taaaacttgc agtagaagaa
     4561 catggagagt atgtagctgt aagggaaatg agaaaacaca tcgcttggta tttaaaagga
     4621 cttagaaact ctgcaaaatt aagggatgaa attaacaaaa tagaagacta tcaagaagtt
     4681 gtgtcgaaat tggagtacta tatggaagac tccttgacat aaaaataata gtgacttata
     4741 atatagcgca taaattaaaa aaagattata aaatttttaa aatataatta ggaaaattcc
     4801 taagaaattt gtgaaggagt tgtatttata atggaagaaa ataaagagtt tttattgaca
     4861 caagagggat atgataaatt agaagaagaa ttagaaaact taaaggttgt aaagagaaaa
     4921 gaagttgcag agagaataaa agttgctata tcttttggtg atttatctga aaatgctgag
     4981 tatgatgaag ctaaaaagga acaagcacaa gttgaagaaa gaattttaaa attagaaaat
     5041 atggttagaa aagctgttat catagatgaa agcaaaatag accttaatgt tgttacaata
     5101 ggatcaatag ttaaagttaa ggatttagaa tttgatgaag aggttgaata tactatagtt
     5161 ggttcgacag aagcagaccc atatgatggt aaaatatcaa acgaatctcc tgtagggaaa
     5221 gcacttttag gtagagctgc gaaagaagtt gtggaagtgc aagttcctga tggagttgct
     5281 aagtttgaaa tattagaaat aagaagataa tttggaggaa aaaaatgaaa aataatcaac
     5341 aaagcaatga agaagcgcaa attcaagaag accttagtga agtacttcaa gtaagaagag
     5401 ataaactaaa aaaattacaa gagtcaggta gagatccttt taaagaaagt agatatgata
     5461 gaactcatta ttctatggat ataaaagata actttgattc tcttgaaggt aaaactacaa
     5521 aaatagcagg acgtataatg agcaagagaa tacaagggaa agctggcttt atagatattc
     5581 aagaccaaga gggaagaatc caatcttatg taagactaga tgctatagga gaggaagaat
     5641 atagtgtatt ttcaacatat gatattggag atatagttgg aattgaagga gaaatcttta
     5701 aaactaaaaa aggtgaaata tctgtaaagg ctaaatctgt agtattacta tgtaaatctt
     5761 tacaagtttt accagaaaaa tatcatggat taaaagacca agaactaaga tatagacaaa
     5821 gatatgttga cttaatagta aatccagaag taaaaaatgc ttttttaatt agaacaaaag
     5881 cattaaaagc attaagagca tacttagatg atagaggatt tttagaagtt gagactccaa
     5941 tattaaatac tatagcaggt ggggcaaatg ctagaccatt tataactaat cataatactt
     6001 tacatatacc aatgtattta agaatagcaa atgaattgta tttaaaaaga cttattgttg
     6061 gtggatttga taaagtttat gaaatgggaa gaatgtttag aaatgaaggt atggatttaa
     6121 aacataatcc tgaatatact gcaatagaat tatatcaagc atatgcagac tatactgata
     6181 tgatggaaat aacagaaaat gttattgcac atatggctga agttgctaca ggaagtatga
     6241 tagttaacta tcaaggaaca gaaataaact ttacaccacc ttggaaaaga atgagtatgg
     6301 aagattgtgt taaagaatat tctggtgtag atttttctac tataaataca gatgaagagg
     6361 cactagaagt tgctagagaa aaaggtatag agataaaacc tggaatgaga agaggagaag
     6421 taataaatgc tttctttgaa gaatttggag aagataaatt aatccaacca acatttataa
     6481 ctcatcatcc agttgaagta tctccattat ctaagagaaa tgttgaagac ccaagaagaa
     6541 ctgatagatt tgaggcattt gctaataagt gggagttagc taatgcattt tctgaactta
     6601 atgaccctat tgaccaaaaa ggaagattta tagaccaatt gagaaaaaga gaattaggtg
     6661 atgatgaagc ttttgaaatg gatgaagatt tcttaaaagc attagaggta ggtctacctc
     6721 ctacaggagg actaggaata ggtatagata gagtaattat gttattaact aactctccat
     6781 ctataagaga tgtattatta ttcccgacta tgaaacctat tgataacaat ccaaataaag
     6841 aagaagaaaa ctaagaaata ttagcattct actaagtttt aaataaagtt tagctattaa
     6901 attagcaata aatagctaaa ttttgtttaa aatgtcagtg tagaatgctt tttatatgct
     6961 tcaaagattc ttttagatat ttaatttata aattaccaac aaacgttaga aattgatatt
     7021 agtttttgtt gattgagact ttatatttag aaaatatgat tgaaagtaat ttattatgaa
     7081 aatatttaga ataatttatg agaaggtaaa aagaataatt tatgagaata ctatatataa
     7141 aaaattattg ggggaatgtc tgatgtttca agtaattaat tttttatgtt tattttgtgt
     7201 tggatttttg gttcttgatg ttatgatata tttttgtaag gatgcaatgg atatttttga
     7261 gggtacaact aatgttaata aagttaagag tataaatatt tgggcagata gagtgagagc
     7321 taattcatat tcttatgaaa ataaaattgc aaaataggta aaattatata tgcatgtatt
     7381 gtttgtaata tcaagggtta tatctattag ttttaattat atttattaaa aaaaccaaaa
     7441 gttataacct tttttaaaat aaaaaacaaa aaaagtattg acgaaatatt tttaag
//
LOCUS       DAMJWP010000177         7492 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.184, whole genome shotgun sequence.
ACCESSION   DAMJWP010000177 DAMJWP010000000
VERSION     DAMJWP010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7492
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.184"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(276..839)
                     /locus_tag="PC700_002892"
     CDS             complement(276..839)
                     /locus_tag="PC700_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582171.1"
                     /translation="MNKVYIVTTYTGTILSYLIRNISKKLYTHVSISLNENLKPMYSF
                     GRLNPRNPFIGGFVEENINQGLYAIRKNTVCRVYSLEVDNLQYENLYKNIKLISDYRE
                     DYYYDTMALIYLLMNIQRQSEYKYVCSNFVADMLEKSEIDILNKQAFEVTPNDFYNLS
                     GLTLEYEGLLSEYNSRQHSYVSKIANI"
     gene            complement(998..1711)
                     /locus_tag="PC700_002893"
     CDS             complement(998..1711)
                     /locus_tag="PC700_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2140 domain-containing protein"
                     /protein_id="HDF2582172.1"
                     /translation="MGKVAKILLSLIVIVLVLAGIVVYILTPKERYDVSSQNNKIIDE
                     EYLSKGSYINSMEVVKNPLRMVGKISVSEEEFKNLIYTLMQKHGINELENNFVEMKNG
                     KIKVAGPYKVFGLVNSQYELELRPTLKDGNLVVSLENVKIGKFKLSDKMLEKILSNYN
                     KKVPFEVNGNKITLEKSYLYPITLKNISIKKGNVDLDMEVEIDLKSQINGALGYLKDS
                     GKVQDILNHLINLKKTSVG"
     gene            2003..3667
                     /locus_tag="PC700_002894"
     CDS             2003..3667
                     /locus_tag="PC700_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HDF2582173.1"
                     /translation="MSNETNSSNFIKNIIINDLETGKHDSIITRFPPEPNGYLHIGHA
                     KSICLNFGLAKEFNGKANLRFDDTNPLKEDVEYVESIKEDVKWLGFDWNELNFASNYF
                     DEMYKRALILIKKGKAYVCDLTQEEMREYRGTLTEPGKESPHRNRTIEENLDLFERMK
                     NGEFKDGEKTLRAKIDMSSPNINLRDPIIYRISHSTHHNTGDKWCIYPMYAFAHPIED
                     AIEGITHSICTLEFEDQRPLYDWFVKECEMENIPRQIEFARLNINNTVMSKRKLKQLV
                     DEGIVDGWDDPRVPTISGIRRKGYTAEALRNFCSEIGVSKVNSTVDSQMLDYFLRENL
                     QPKAPLTMGVLRPLKLIITNYPEDKIEMLEIENNAKDESQGKRLVPFSRELYIEQDDF
                     MEEPVKKYFRFFPGNEVRLKGAYFVKCTDVIKDENGNVVEIHGTYDPETKSGSGFTGR
                     KVKSTIHWVDAKSAIPCEFRLFEPLILDDIPENEGKHFLEQINPNSLEILQGFVEPTQ
                     IKDSKPFDKFQFVRNGFFSIDNKYTTDEKFVFNRIVPLKSSFKPGK"
     gene            complement(3774..4673)
                     /locus_tag="PC700_002895"
     CDS             complement(3774..4673)
                     /locus_tag="PC700_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HDF2582174.1"
                     /translation="MKKVKLIYNPNSGEKKILSNLDTIIELYQEKNYLLIPYRLTIKE
                     PVKNAFKEVDSSYEHILIAGGDGTVDLVVNAMKELDIKIPIGILPTGTANDFSNALQI
                     SFDIKEAINEIINSKPKKIDIGKVNNKYFINVASAGMFTDVSQRINTDLKNSFGRISY
                     YIKGIEEALYMRKFKIKVSSEEMYYDGDMYLMLIFNGKTAGNINLAYKAEIDDGYLDV
                     IIFKGMPIPKSIPVLISVLRGDYLDQYNGNEILYFKTKKVHIECEDSLITDIDGEKGP
                     DFPLNIECIKDGIEVMGICSEKS"
     gene            complement(4700..5338)
                     /locus_tag="PC700_002896"
     CDS             complement(4700..5338)
                     /locus_tag="PC700_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HDF2582175.1"
                     /translation="MDQNLNWLNEKRIEKTIKSLENNNMKGYLVSNKEELLKKIEEIV
                     KVGSLVGCGGSMTLFETGVIDFIREKYNFLDRYAKDIKPEEMKEIYRKSFSADAYFTS
                     TNAITEDGELYNLDGNGNRVAAMIYGPDKVIVIAGVNKIVKDVDEAIKRTKTIAAPAN
                     CKRLSTNTGCKTTGICIDCSSPGRICCSYTLIKRQLTPERIHVIFLNEDFGY"
     gene            complement(5675..6673)
                     /locus_tag="PC700_002897"
     CDS             complement(5675..6673)
                     /locus_tag="PC700_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4003 domain-containing protein"
                     /protein_id="HDF2582176.1"
                     /translation="MEQSFINGKVNLLIENYKALNEVKGSWQMGLIQHSCALAFTLKN
                     KRISPRLVEERIELIKKNTGLFSNFRGYNMFYMATLLSFESNPESSFKMILDIYKELK
                     SEKFWGDTYLPLTASIVYENREKMDYLTCISKMKIIYDYMRKKHPFLTSSDDYCNIAL
                     IAIHSKNLDEDLEYIEKCYEFLNENGFYKGNDLQALSQILLFSDDRTMLKCKKTIELK
                     KAFKENDCKMNYYGYPIIGAISLLDYREDEIIEEIKNVSNKLKEEKGFGNWSLGKSNR
                     VMISSAIVASIYADLIRNENNIGSITNNIFLNIVVAIQIACVMAATSAAIASSSSN"
     gene            complement(6771..7346)
                     /locus_tag="PC700_002898"
     CDS             complement(6771..7346)
                     /locus_tag="PC700_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582177.1"
                     /translation="MKLKEALITCVVCSVCLTPLLVNGETKIDADHCKESIVNIDTIV
                     KSGKSFNSKYNQGMEDYKNSDEYKLKVQKEKLQNQLGIKIKKLVPITFEVSYYTSLNQ
                     ENGYGAITATGDDLEDGFVANNHLSFGTKIIVDGKLKTVMDRGSSKYFSNSNAIDVFV
                     PKWSNESESEYYDRVNNMGRHYKKGYIIVEE"
BASE COUNT         2558 a         1177 c          817 g         2940 t
ORIGIN      
        1 caactatatt taacattata cgtgtatgta taaatacttt ttatacactg tttgtgctat
       61 ccatcccgca cctaaaggag tgggattttt gcactattct gtattatata tatccaaaaa
      121 tattataaaa atacaaatat tagcagaaat ttaaaaactg aagaaagtat tctaactaag
      181 atttacaaat tcttaatcct atcgaagtta aactactatc ttcagcctaa ttatctagca
      241 atattttttt atatgaaaaa ctctaaatca agtttttaaa tgtttgcgat ttttgataca
      301 taagaatgtt gtctagaatt atattctgat aataaacctt catactctaa ggttaagcca
      361 gataaattat aaaaatcatt tggcgttact tcaaatgctt gtttatttaa aatgtctatc
      421 tcactttttt ctaacatgtc agcaacaaag tttgaacata catatttata ttcagattgt
      481 ctctgaatat tcattagtaa atatattaat gccattgtat cgtaatagta atcttctcta
      541 taatcactaa ttaattttat atttttatat agattttcat attgtaaatt atctacttct
      601 aacgaatata ccctacacac agtgtttttt cttatagcat acaatccttg atttatattt
      661 tcttcaacaa atcctccaat aaatggattc cttggattta gccttccaaa agaatacatt
      721 ggttttagat tttcatttaa tgatattgac acatgtgtat acagcttttt acttatattc
      781 cttattaagt aagataatat agttcctgta tatgttgtta caatatagac tttattcatc
      841 atttcccccc aatctagtaa ctaaatgata ttatacactt acaatttaat atcataagat
      901 attctaacat aataaatgtg gttttacaaa ttttgatttt aaggtacttt aatattatat
      961 aatataataa aaatataaat ataagttttc tatattttta accaacagat gttttcttca
     1021 aattaatcag atgatttagt atatcttgaa cctttccaga gtcctttaaa tatcctaatg
     1081 ccccatttat ttgtgatttt aaatctattt ctacttccat atccaaatca acatttcctt
     1141 tttttataga tatattttta agtgttattg ggtaaagata acttttttcc aaagtaatct
     1201 tatttccatt aacctcaaat ggaacttttt tattgtagtt acttaatatt ttttctagca
     1261 ttttatcact tagtttaaat tttccaattt tcacattctc aagacttaca actaagtttc
     1321 catcctttaa agtaggtctc aattcaagtt catattgact atttaccaaa ccaaatacct
     1381 tatatggtcc tgctaccttt attttaccat ttttcatttc aacaaaatta ttttcaagtt
     1441 cattaattcc atgcttctgc atcaatgtat atattaaatt tttaaactct tcctctgaaa
     1501 cagatatttt tccaaccatt ctaagtggat tttttactac ctccatagaa tttatatatg
     1561 agccttttga taaatattct tcatctatga ttttattatt ttgtgaacta acatcatatc
     1621 tttcttttgg tgtcaagata tatacaacaa ttcctgcaag tactaaaaca ataacaatta
     1681 aacttaacaa aatctttgca acctttccca taatcgtctc ctttacaaat ctgtcatcag
     1741 atttaaaata aatttagata actaacttca cataatattc ttaacaatat acatactata
     1801 atacagtctt ttaaaaatta attcaaccaa tctaatgttt tgtaaataaa ctgtaaaaac
     1861 atgtttttag tataaaagtt aatattttgc ataatactct taattacacc tttatttttt
     1921 tctaatcttg gattaaaatt aatattatgc aatacagtgt agtgttctgt attataatca
     1981 aattaaatag gaggtataga atatgtcaaa tgaaacgaac tcatctaact ttataaagaa
     2041 tattattata aacgacttag aaacaggaaa acatgacagt atcataactc gttttccacc
     2101 tgagccaaac ggatatttac atattggtca tgcaaaaagt atatgtctta attttggatt
     2161 agctaaggag tttaatggaa aagctaattt aagatttgat gatacaaacc cattaaaaga
     2221 ggatgttgaa tatgtagagt ccataaaaga agatgtaaag tggttaggat ttgattggaa
     2281 cgagttaaat tttgcatcta actactttga tgaaatgtat aagagagctt taatactaat
     2341 aaaaaagggt aaagcttatg tatgtgattt gacacaagag gaaatgagag aatatcgtgg
     2401 tactctaact gaaccaggaa aagaaagtcc tcatagaaac agaactattg aagaaaacct
     2461 tgatttattt gaaagaatga aaaatggtga atttaaagat ggtgaaaaaa cattaagagc
     2521 caaaatagat atgtcttctc caaatataaa ccttagagac ccaattatat atagaatatc
     2581 tcattcaaca caccataata caggagataa atggtgcata tatcctatgt atgcttttgc
     2641 tcatccaatt gaagatgcaa ttgaaggaat aactcactct atatgtactt tagagtttga
     2701 agaccaaaga cctctttatg attggtttgt taaagaatgt gagatggaaa atatacctag
     2761 acaaatagaa tttgctagac ttaatataaa taatactgtc atgagtaaaa ggaaattaaa
     2821 acaacttgta gatgagggta ttgttgatgg ttgggatgac cctcgtgttc ctacaatatc
     2881 cggaatcaga cgtaagggat atactgcaga agcattgcgc aatttctgta gtgaaatagg
     2941 ggtatcaaaa gttaactcta cggtagatag tcaaatgctt gattatttcc taagagaaaa
     3001 cttacaacca aaggctcctc ttactatggg agttttaaga cctttaaaac ttattataac
     3061 taattaccca gaagataaga ttgagatgct tgaaatagag aataatgcta aagatgaatc
     3121 tcaaggaaag agattagttc ctttctctag agaattgtat atagaacaag atgattttat
     3181 ggaagaacct gttaagaaat attttagatt tttcccagga aatgaagtcc gtttaaaagg
     3241 tgcttatttt gtaaaatgta ctgatgtaat taaggatgaa aatggaaatg tagttgaaat
     3301 acatggtact tatgaccctg aaactaagag tggctctggc tttacaggac gtaaggtaaa
     3361 atctacaata cattgggttg atgctaaatc agctatacca tgtgaattta gattatttga
     3421 accattaatt cttgatgata tacctgaaaa tgaaggtaaa catttcttag aacaaataaa
     3481 tcctaattca ttggaaatat tacaaggttt tgttgaacct acacagataa aagattctaa
     3541 accttttgat aaattccaat ttgttagaaa tggattcttt agtatagata ataaatatac
     3601 aactgacgaa aaatttgtat ttaatagaat agttcctcta aaaagttctt ttaaaccagg
     3661 taaataatct tacaatcaaa aaatgaagac ttaaaatata aattacttct actataaata
     3721 aacttagtta gaagtaattt tttatttgag tcttcatcat ttactttaaa tgattaactt
     3781 ttttcactac agattcccat aacttctatt ccatctttta tgcattctat gttaagagga
     3841 aaatctggtc ctttttctcc gtcaatatct gttataagtg aatcttcaca ttctatatgc
     3901 acctttttag ttttaaagta tagtatttca tttccattat attgatctaa ataatcacct
     3961 ctaagcacac ttatcaaaac tggtattgat tttggtattg gcattccttt aaaaataatt
     4021 acatccaaat atccatcatc tatttcagcc ttatatgcta gatttatatt tcctgctgtt
     4081 ttgccattaa aaatcaacat aagatacata tctccatcat aatacatttc ctcagaagat
     4141 acttttatct taaatttcct catatacaat gcttcttcta ttccctttat ataataagaa
     4201 attcttccaa atgaattttt taagtctgtg tttatccttt gtgatacatc tgtaaacata
     4261 ccagcacttg caacatttat aaagtactta ttattgactt tccctatgtc tattttcttt
     4321 ggttttgaat ttatgatttc atttatagct tcttttatat caaatgaaat ttgaagagca
     4381 tttgaaaaat catttgcagt tcctgtaggc aaaattccaa ttggaatttt tatgtctaat
     4441 tctttcattg catttactac taaatctaca gtaccatctc caccagctat caatatgtgt
     4501 tcataagaac tatcaacttc cttaaatgca ttctttacag gttctttaat agttaatcta
     4561 tagggtatca ataaataatt tttttcttga tataattcta tgatagtgtc taaatttgat
     4621 aatatctttt tttcacctga attaggattg tatataagtt ttactttttt cattatttct
     4681 ccttactcat tttattcttt tagtatccaa aatcttcatt taaaaaaatc acatgtattc
     4741 tttctggtgt taattgtctt tttataagtg tataactaca gcatattctt cctggacttg
     4801 aacagtctat acatatacca gtagtcttac atccagtatt tgtacttaat cttttacaat
     4861 tggctggagc agctattgtt tttgttcttt ttatagcttc atctacatct ttaactattt
     4921 tatttactcc tgctataact attactttat caggtccata tatcatagca gcaactctat
     4981 tcccattccc atctaaattg taaagttctc catcctctgt tatcgcatta gtacttgtaa
     5041 aataagcatc tgcagaaaat gactttctat atatttcttt catttcttct ggtttgatat
     5101 cttttgcata tctatcaaga aaattatatt tttctcttat aaaatcaatc actcctgttt
     5161 caaataaggt catagaacct ccacatccta ctagagaacc tactttaact atttcttcaa
     5221 tttttttaag aagctcttct ttgttactta ctaaataccc tttcatatta ttgttttcca
     5281 gagattttat tgttttttct attctttttt cattaagcca atttaaattt tgatccattt
     5341 taacttcccc cttattttta atatgtactt aattatttat aaactttatt gtctttataa
     5401 gtttatttta gtacatctaa aaaaaaatac aatataaaac ttaaatagtt ttatataatt
     5461 ttttccaaaa taacttactc aatcaaaaaa ggaaagtaga tgtactattc tactttccct
     5521 atattgtatg tagtatttat tgcctaaaat ctatatatta attatatgtt tcatattata
     5581 taatatcaca gttaattaaa atgtaatact atcttctttt taaacatata aattgtatta
     5641 ttaaatttaa tatatccaac ttcaataaat cttattaatt tgatgaagaa gatgctatag
     5701 ctgcacttgt tgctgccatt acacatgcaa tttgaatagc aacaacaata tttaaaaata
     5761 tattattagt tatagagcct atattattct catttcgtat caaatctgca taaattgaag
     5821 ctacaatagc agaactaatc attactctat tactttttcc taaagaccaa tttccaaagc
     5881 ctttttcttc tttaagttta tttgaaacat tttttatctc ttctattatt tcatcttctc
     5941 tataatctaa taatgaaatt gctcctatta ttggatatcc ataataattc attttacaat
     6001 cattttcttt aaatgctttt ttaagttcaa tagtcttttt acatttaagc atagttctat
     6061 catcactaaa taaaagaatt tgggataatg cttgtaaatc atttccttta taaaaaccat
     6121 tttcatttag aaattcataa catttttcta tgtattctaa atcttcatct aaattttttg
     6181 aatgaattgc aataagtgct atattacaat agtcatcact agaagttaga aatggatgtt
     6241 ttttcctcat gtaatcatat attattttca tttttgatat acatgtaaga taatccattt
     6301 tctctctatt ttcataaact attgatgctg taagtggtaa atatgtatcg ccccaaaact
     6361 tttcactttt aagttctttg tatatatcta aaatcatctt aaaactactt tctggatttg
     6421 attcaaacga aagtagtgtt gccatataaa acatattata tcctctaaaa ttagagaata
     6481 atccagtatt ttttttaata agctctattc gttcttccac taatctagga cttattctct
     6541 tgttcttaag agtaaatgca agtgcacaac tatgttgtat caatcccatt tgccaagaac
     6601 ctttaacttc atttaatgct ttgtaatttt ctattaataa gtttactttt ccatttataa
     6661 acgattgttc catattgccc tcctagtaat atatctcatt gtactaatag ttttatattt
     6721 attatatata tattaaatac tacaatcaat acatattact tcaaagaaca tcattcttca
     6781 acaataatgt agcctttttt gtaatgtctt cccatattat tgactctatc atagtactct
     6841 gattcacttt cattactcca tttgggcaca aatacatcta ttgcattaga attactaaaa
     6901 tacttactag accccctatc cataacagtt tttaattttc catctacaat aatcttagtt
     6961 ccaaaactca agtgattgtt tgctacaaaa ccatcttcta aatcatctcc tgtagcagtt
     7021 atcgctccat atccattttc ttgattaaga cttgtgtaat aactaacttc aaaagttata
     7081 ggtactaact tctttatttt aatacctaac tgattttgta gtttctcctt ttgtactttc
     7141 aacttatact catcactatt tttataatct tccatacctt gattatactt ggaattaaag
     7201 cttttacctg actttactat agtatctata tttactatag attctttaca atgatctgca
     7261 tctatttttg tttctccatt taccaataat ggagttaaac atacactaca tacaacacat
     7321 gtaatcaatg cttctttaag cttcataaat tcccccctta ttattttgat atattagcat
     7381 atttaatatt atatgtctat atttagattt aaataccaca cccatttgct ttaagaagtt
     7441 gtcttatatt taattcacct taaacactta taaatcctct atcttagaat ca
//
LOCUS       DAMJWP010000178         7480 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.185, whole genome shotgun sequence.
ACCESSION   DAMJWP010000178 DAMJWP010000000
VERSION     DAMJWP010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7480
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.185"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(123..863)
                     /locus_tag="PC700_002899"
     CDS             complement(123..863)
                     /locus_tag="PC700_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDF2582178.1"
                     /translation="MLYDEDEKEENNERWLLTYSDLITLLMIFFVIMYSMSNVDAEKY
                     KQLSQSLNSAFGGSSGVIEGGNSKIEPVVEPGSNDLDSLQNAKFKKVGEEIQKYLNEN
                     GMANSVSLRVQDRGLVISLKDTILFDTGKAVVKDNSRDKIIQIGKMLNEMNSYMRVEG
                     HTDNMSIKNSEFKSNWDLSVMRATNVVQLLIDNAGIAPDKLSAVGYGEFRPIAENSSE
                     EGRSKNRRVDIVLVDSKYDNVENVSKDK"
     gene            complement(884..1672)
                     /locus_tag="PC700_002900"
     CDS             complement(884..1672)
                     /locus_tag="PC700_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HDF2582179.1"
                     /translation="MTTLIFFIVGIASLVVAFVIDGGHIGVLFVFSSALVVFGGTIGA
                     VGASTPFPVFKKSIKIMLLAFKNKKPNNIENIIYFKEIATKARKEGLLSVDKEISEND
                     ELDPFIRKGLELMVDGVDAVTIRNILESQIQITSKRHKEGIAIFDSAGGYAPTLGIIG
                     TVMGLVHILGNLSSNPDALGPQISVAFLATLYGVGSANLIWLPIASKLKAINQQEISE
                     RLLVIEAILSILEGDNSNLMTEKLKIFLNSKELIELEEIGGRAE"
     gene            complement(1760..1850)
                     /locus_tag="PC700_002901"
     tRNA            complement(1760..1850)
                     /locus_tag="PC700_002901"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1814..1816),aa:Ser,seq:gct)
     gene            2109..2873
                     /locus_tag="PC700_002902"
     CDS             2109..2873
                     /locus_tag="PC700_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDF2582180.1"
                     /translation="MLPQERYEKILSILKSDNIIKINDIVQMFDISIETARRDLAHME
                     SIGLLKRVYGGAIPVIASSVELNYVKRKKINSNDKKQIAIKCSEFINTNDTIFMDTGT
                     TVLEVSKNLKDKKNLTVITNSILVANELMNTNIKVYVLGGYLRETEGSFSGPLTLSAI
                     EQFNVDKAILGAGGITLENGISDYHLEEALVRKKMIERSRKSIVVVEPPKFGINSFAS
                     VAPIEHIDTIITSASIDHNIIIEFSQKGIPLVIADN"
     gene            complement(3154..4269)
                     /locus_tag="PC700_002903"
     CDS             complement(3154..4269)
                     /locus_tag="PC700_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HDF2582181.1"
                     /translation="MPFDKIILYIMAVGVLLGALDRIIGNKFGLGEQFEEGFNSMGPL
                     ALGMVGIVCLAPVISDVLGPIIIPIFNLCGADPSMFATILANDMGGYPLAMELAHNKE
                     AGMLAGLVVSSMLGCTLVFSIPVGLGLIEYEDRPFFAKGLLIGLITIPFGGIIGGLIA
                     GFNLSMVIINMIPVIILAVILAVGLKFNSTLMINGSLVFGKFISILITIGLGAAAFQE
                     ITGIVLIKGMTPIREGIQVIGSIAIVLLGTFPILHLLVKVLNKPLTIVGGKLGMDATS
                     AAGLVFTLANSIPVYKMMKDMSPKGKVVNTAWLVCATAALGDHLGFTAGVVPEMITPV
                     VIGKLVGGVLAIALAILMTKDLTLEEEQSIKMKKDAV"
     gene            complement(4310..5836)
                     /gene="glpK"
                     /locus_tag="PC700_002904"
     CDS             complement(4310..5836)
                     /gene="glpK"
                     /locus_tag="PC700_002904"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDF2582182.1"
                     /translation="MKYVLAIDQGTTGTRAVLINEKSEFVFSDYMEHTQIYPQSGYVE
                     HDPIEIWNNTKTVSNNVLQQAFESGINESDIKGIGIDNQGETVMLWDKNTGKPLYNAI
                     VWQCRRTYDYVENLKNIPGLENKIIDKTGLIIDPYFSGTKIKWVIDNVKGVKEKIKNG
                     EVLAGTLDSWLIWKMTGGKSFLTDVSTAARTMLFNIRDMDWDRELLDIIGVPRSILAD
                     IMPTSGDFGTTDKEAFCGVSIKITASMVDQPAALFGNGCFEPGMVKNTYGTGCFLYMN
                     IGEELNIAGNGILTTVAWKIGDKVTYAYDGGVYIAGAAIQWLRDGIGVIKNYSETDDM
                     ANSISSTGGVYFVPAFAGIAAPYWDQYARGTMVGITGSTKKEHIVRATLESVALQVKD
                     VVDSMNLASGKEIKMLRVDGGITKNNFTMQFQSDILDIPIEIAENPETTVLGVGYMAG
                     LSCGVWNSIDEIRSEFTISKKYIPNMTKQKREEILLGWNQAVKRAMNWENDTKKVCAN
                     "
     gene            6129..7226
                     /locus_tag="PC700_002905"
     CDS             6129..7226
                     /locus_tag="PC700_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDF2582183.1"
                     /translation="MKKTYLFSPGPVMVTDKVRNSLLHYDICHRGNEFMDLFKDTQQK
                     INKLYNATSNYYSLIVSGSGTSVNECVLSSIFDKEDAVLLVSNGVFGERLEEILFTYN
                     VKTYKPNFEWAEYPDLDILEKYIIENSDIKVIAMVFHETSSGMINPVPELGKLAKKYN
                     KIFFVDAISASAGEYIDVEEFNIDIITGVGGKALGAFPGSAYLCAKESILQNIKENQC
                     KNVYLNLYKHYKVAKSSSQTPNTPNVTLIFALNEALTEFLEDDSKIERYKECSAILRN
                     GMEELGLTFLLPDKYMSNTVTSVFLPKEINVNEFILGLERESGYVVYPGKGKFLDANI
                     FQVANMGEIYPEDCYKFLDILKSKLEIKKVI"
BASE COUNT         2481 a         1338 c          909 g         2752 t
ORIGIN      
        1 ttatatattt aagataccat atattcctac aaaaaggtaa cttactatgt atctattttt
       61 aattttcgca taaaaaagcc atctccttta gaattaattc tgtattgaga cagctaattt
      121 aactatttat cctttgaaac attttctaca ttatcatatt tactatcaac taaaactata
      181 tcaactcttc tattttttga cctaccttct tcagatgaat tttctgctat tggtctaaac
      241 tcaccatatc ctacagcaga taatttatct ggtgctatac ctgcattgtc aataagaagt
      301 tgaacaacat tggttgctct cataactgat aaatcccaat ttgacttaaa ctctgaattt
      361 tttatagaca tattgtctgt atgaccttct actctcatgt aactattcat ttcatttaac
      421 atcttaccta tttgaataat cttatctcta gaattatcct ttacaactgc ttttcctgta
      481 tcaaatagta ttgtatcttt aaggcttatt actaatcctc tatcttgaac tcttaatgat
      541 actgaattag ccataccatt ttcatttaaa tacttctgga tttcttcacc aactttttta
      601 aacttagcat tttgtaaact atctaaatca ttacttcctg gttcaactac tggttctatt
      661 ttgctattac caccttcaat aactccagaa gaaccaccaa aagcagaatt tagagattga
      721 gataattgtt tatatttctc cgcatcaaca ttactcatag aatacattat tacaaagaaa
      781 atcataagta atgttatgag gtctgaataa gttaaaagcc atcgctcatt gttttcttct
      841 ttttcatctt catcatacaa cataaacatt ccatcctttt atattattca gctctaccac
      901 caatttcttc aagttcaatt agttcttttg aatttaaaaa tatttttaat ttttctgtca
      961 ttaaattaga attatcaccc tcaagtatgg ataatattgc ttctatcacc aaaagtcttt
     1021 cacttatttc ctgttgattt atagccttaa gttttgatgc tattggtaac catattaaat
     1081 tggcagaacc aacaccatac aaagtagcca aaaatgcaac agaaatttga ggtcctaatg
     1141 catctggatt acttgaaaga ttacctaaaa tatgtactaa tcccataact gtacctatta
     1201 tccccagtgt aggtgcatat ccacctgctg aatcaaaaat tgctattcct tctttatgtc
     1261 gttttgatgt tatctgtatc tgtgattcca atatatttct tatagtaact gcatccaccc
     1321 catctaccat aagttcaagc ccttttctaa taaaagggtc taattcatca ttctcactta
     1381 tttctttgtc aacactaaga agcccctctt tcctagcctt tgttgcaatt tctttgaaat
     1441 agattatatt ttctatgtta ttaggctttt tatttttaaa agctaataac attatcttta
     1501 tagacttttt aaacacaggg aatggagtcg aagctccaac agcgcctata gttccaccaa
     1561 acacaacaag tgctgatgaa aatacaaata atactccaat atgtccccca tcaataacaa
     1621 aagcaactac caaagatgct atccccacta tgaaaaaaat taaagttgtc atctattccc
     1681 tcctttcttt aattatacat atctaatcat ttatgacatt ataaaaagac tatctttaat
     1741 aaaaatagcc tttaacaatt ggcggagaag gagggattcg aaccctcgca ccggttaccc
     1801 agcctaatcc cttagcaggg gatcctcttg agccacttga gtacttctcc atatctctat
     1861 actagtaaat tatataataa aatcatattt tagtcaattg ttttataaaa tttgagttat
     1921 tttgaattat taaacatcta tttttacttt tttgcttaaa tgtaagtttt aaatatggta
     1981 aaattatctt gcataacact tataatatta tttgataaat atacatacat taacaaatat
     2041 acctaaactc tgacttaatt gtaagttatg ccaaaactaa aataatacta gtccaaggag
     2101 gatattttat gttaccacag gaaagatacg agaagatttt aagtattctc aaatcagata
     2161 atataattaa aataaatgac atagttcaaa tgtttgatat atcaatagaa actgcacgaa
     2221 gagatttagc ccatatggaa agtatcggtc ttttaaaaag agtttatggt ggtgctatac
     2281 cagttatagc aagtagcgta gaacttaact atgttaaaag aaaaaaaata aactcaaacg
     2341 ataaaaaaca gattgctatt aaatgtagtg agtttataaa tacaaatgat actattttta
     2401 tggatactgg aactactgta cttgaagttt caaaaaattt gaaagataaa aagaatctaa
     2461 ctgtaataac caactcaatt ttagttgcaa atgaacttat gaatactaat ataaaagtat
     2521 atgtgcttgg tggctatctt agagaaactg aaggttcttt ttctggtcca cttacactct
     2581 cagctattga acaatttaat gttgataaag ctattttagg tgctggaggt atcactcttg
     2641 aaaatggaat ctctgactat cacttagaag aagcactggt tagaaaaaaa atgattgaaa
     2701 ggtctagaaa atctatagta gttgtcgaac ctcctaagtt tggtataaat tcatttgcat
     2761 cagtagctcc tatagaacac atagatacta ttattacctc tgctagtata gaccataata
     2821 taataataga gttttctcaa aaaggaatac ctttagtcat agctgataat tagcttgtat
     2881 gattgatttt tagttccttg atgtaataaa ttaaaattaa ggagccaaat aattatttat
     2941 ttagctccac ttatgtctaa actatacttt atattttaat tatgtaacta ctttcagtta
     3001 gcttatcatt tttagttatt tgattgccat tttatttaat ttatcagttt tggttattcg
     3061 attatttttt aattgtctgt ctttttaatt tatttttagt tatttaaact cctactttaa
     3121 aataacttaa atttgactta agtattgttt attctacact gcatctttct tcattttaat
     3181 actttgttct tcttccaaag ttaaatcttt tgtcataagt atagcaagtg caattgccaa
     3241 tactcctcca accaattttc caattacaac aggtgtaatc atctctggaa ctactcctgc
     3301 tgtaaatcct aaatggtccc ccaaagctgc tgtagcacaa accagccaag cagtatttac
     3361 tactttcccc tttggactca tatctttcat cattttatat actggaatac tattggctaa
     3421 agtaaaaact aatcctgctg cactagtggc atccatacct aattttccac ccactattgt
     3481 aagtggttta ttaagaactt taactaataa atgtaatatt ggaaaagtac ctaataacac
     3541 tattgcaata cttccaatta cttgaatacc ttctcttata ggtgtcatgc cttttataag
     3601 tactatacct gttatttctt gaaatgctgc tgctcccaaa cctatcgtta tcaatataga
     3661 tataaatttt ccaaaaacta atgacccatt tatcataagt gtagaattaa attttaaacc
     3721 tactgccaat attactgcca atattatcac tggtatcata tttataatta ccattgataa
     3781 gttaaatcct gctattaatc ctcctataat acctccaaaa ggtattgtta tcaagcctat
     3841 cagtaaccct tttgcaaaga atggtctgtc ttcatattct ataagaccta gtcctactgg
     3901 aattgaaaat actaatgtac atccaagcat ggatgacacg actaatcctg caagcattcc
     3961 tgcctcttta ttgtgtgcaa gttccatagc caatggatac cctcccatat cattcgctaa
     4021 aatagttgca aacatagatg ggtcagctcc acagagattg aatattggta ttattatagg
     4081 acctagtaca tctgaaatta ctggagctaa acaaactata ccaaccattc ctaaagccaa
     4141 tggacccatt gaattaaacc cttcttcaaa ctgctctcca agtccaaatt tatttccaat
     4201 tattctatct aacgctccta aaagcactcc cacagccatt atgtataata taattttatc
     4261 aaagggcatt tttaattcct cctatacatg taaaatactc tattgtaact taattagcac
     4321 acaccttttt ggtatcgttt tcccaattca tagctctttt tactgcttga ttccatccaa
     4381 gtaatatttc ttctcttttt tgcttagtca tatttggaat atattttttg cttattgtaa
     4441 attcacttct aatttcatca atactgttcc aaacaccaca ggaaagccct gccatatacc
     4501 ctacaccaag tactgtcgtt tcaggatttt ctgcaatttc aattggtata tctaatatgt
     4561 cagattgaaa ttgcattgta aagttatttt tagtaattcc accatctact ctcaacattt
     4621 ttatttcttt ccctgaagcc aaattcatgg aatcaacaac atccttgact tgaagtgcaa
     4681 cagactcaag agtagccctt actatatgtt cttttttagt acttcctgtt atcccaacca
     4741 ttgtgcctct agcatattgg tcccaataag gtgctgctat acctgcaaat gctggtacaa
     4801 aatatacccc tccagtactt gatatggaat ttgccatgtc atcagtttct gaataatttt
     4861 ttataactcc aattccatct ctaagccatt gtattgcagc acctgctata tacactccac
     4921 catcataagc atatgtaact ttatctccta tcttccaagc aactgttgtc aaaataccat
     4981 tacctgctat atttaactcc tcacctatat tcatatataa gaaacatcct gtaccataag
     5041 tatttttaac cattcctggt tcaaaacatc cattgccaaa taaagctgct ggttggtcta
     5101 ccattgaagc agtaattttt atagaaaccc cacaaaatgc ctccttatct gtagttccaa
     5161 aatcaccact agtaggcatt atatctgcta aaatacttct aggcactcct ataatgtcaa
     5221 gcaactctct atcccaatcc atatctctta tattaaaaag cattgttctt gctgctgtag
     5281 atacatcagt taaaaaagat tttcctcctg tcattttcca tattaaccaa ctatccaatg
     5341 ttcctgcaag tacttctcca tttttaattt tttcttttac accttttaca ttatctatta
     5401 cccatttaat ttttgtacct gagaaatatg ggtctataat taacccagtc ttatctatta
     5461 tcttattttc taaacctggt atattcttta aattttccac ataatcataa gttctcctac
     5521 actgccatac gatagcattg taaagtggtt tacctgtatt tttatcccat aacattactg
     5581 tttcaccttg attatctata ccaattcctt ttatatcact ttcattgatt ccactttcaa
     5641 aagcctgttg taatacatta ttactcacag tttttgtatt attccaaatt tctatagggt
     5701 catgttcaac atatccagat tgtggatata tttgagtatg ttccatataa tcgctaaaaa
     5761 caaactctga tttttcgttt atcaatactg ctcttgttcc tgttgttcct tggtcaattg
     5821 ctaatacata tttcacttaa aatgtccccc tttattttca gaattaataa attctttttt
     5881 attttttgaa tatataatct attcaagtta tttatatttt atatcatcat gtggatttat
     5941 gcaatattca ttacaatatt ttttgttatt tttaattatg gtgattaaaa aagtattttt
     6001 attgaaatat tattgcaata ttcattattt tattctataa tctagttaaa ataacacaca
     6061 atattttaat atttacacat agtcagtcat tcgaaaattt aaatttaact ttttaaggag
     6121 gctttacaat gaaaaaaaca tatctattca gtcctggacc agtaatggtt acagataaag
     6181 tcagaaattc acttcttcac tatgatatat gccatagagg aaacgagttt atggatttat
     6241 tcaaagatac acaacaaaaa atcaataaac tatataatgc aacttctaat tactactcat
     6301 taattgtatc tggctctgga acatctgtaa atgaatgtgt actatcttct atctttgaca
     6361 aagaagatgc agttctgcta gttagtaatg gtgtttttgg agaaaggcta gaagaaatat
     6421 tatttactta taatgtaaaa acatataagc ctaattttga atgggcagaa tacccagacc
     6481 tggacatttt agaaaaatat ataattgaaa attctgatat aaaagtaatt gctatggtgt
     6541 tccatgaaac tagttcagga atgataaatc cagtccctga actaggaaaa cttgctaaga
     6601 agtataacaa aatatttttt gtagatgcaa taagtgcctc agcaggtgaa tatattgatg
     6661 tagaagagtt taatatagat ataataacag gtgttggtgg taaagctctt ggagcatttc
     6721 caggttctgc atacctttgt gctaaagaga gtattttaca aaatattaaa gaaaatcaat
     6781 gtaagaatgt atatcttaat ctttataaac actataaagt tgctaaatca tcttctcaaa
     6841 caccaaatac acctaatgtt actttaatat ttgcccttaa tgaagcttta actgagtttt
     6901 tggaagatga cagtaaaata gaaagataca aagaatgttc tgctatttta aggaatggca
     6961 tggaagaatt ggggctaaca tttttacttc cagataagta tatgtctaat acagtaacat
     7021 ctgtatttct acctaaagaa atcaatgtaa atgagtttat attaggccta gaaagggaaa
     7081 gtggctatgt tgtctatcca ggaaaaggca aattcctaga tgctaacata ttccaagttg
     7141 caaatatggg agaaatatat ccagaagatt gttataaatt cttagatatc ttaaaatcaa
     7201 aattggagat aaaaaaagtt atataacagt ttgtttgtaa aatcctacaa tacatcagta
     7261 taaattaaat agactgattt ttttaaattt tcctataata aattatattt tatatttttg
     7321 tataagggtg aactataata tgtaaagttc acccctattt caaatctttt tccttcagta
     7381 taatcttttt cttttaattt attttatatt tttgacataa ttattcttac atttaattgt
     7441 tttttatttt tgacgcaatc gttttttatt cagtcatttt
//
LOCUS       DAMJWP010000179         7442 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.186, whole genome shotgun sequence.
ACCESSION   DAMJWP010000179 DAMJWP010000000
VERSION     DAMJWP010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7442
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.186"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            122..793
                     /locus_tag="PC700_002906"
     CDS             122..793
                     /locus_tag="PC700_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2582184.1"
                     /translation="MNLLIIEDDINLNEGLFYAFENDGFNVFKAYTKQEGLNIFNSKN
                     IDFIILDCNLPDGDGFDVCQIIREKSDIPIIMLTARDSEIDEVKGLEIGLDDYITKPF
                     SLSVLKARVKVAIRKKSNNKVIYSNGIKLDQKLLKVYKNKACLELSSVEYKLLSYLIE
                     NKGQILLKEQILHHIWDSEENYVDDNIVSVNIRRLRVKVEDDPSNPKYIKTAYGMGYL
                     WNEVE"
     gene            795..1703
                     /locus_tag="PC700_002907"
     CDS             795..1703
                     /locus_tag="PC700_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2582185.1"
                     /translation="MFILAFIICTVVIFIVSTKINQRRYDELNLMLNQILEGKEVTYP
                     DTKDTRASKISHQVKKIKDMIEIEVEQSKSEKEAIKGLISNMSHQLKTPLSNITIYCE
                     LLENINISTLQKKEFLQKMKNETFKIDWLLQSLFKMTKLEDGVIEFEVEELLIKDTLI
                     QSISTIFNKSEAKNIRINLEPFSDIKLVHNKKWTIEAIVNVLENAIKYSPSDSTITIS
                     VIKMELYTKITIKDEGIGIDSRELNDIFKRFYRSKNVENQNGTGIGLYLTRLILEKEN
                     GNIIVESKLGSGCCFSIFLQNCKSLN"
     gene            1780..2466
                     /locus_tag="PC700_002908"
     CDS             1780..2466
                     /locus_tag="PC700_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2582186.1"
                     /translation="MSILVTNNLVKHYGKGETKVKALNGVSIEIERDTFTAIVGTSGS
                     GKSTLLNIIGGLDNPTSGDVIIKGKNISKIGKKDLTVFRRRNIGFIFQNYSLMPVLNT
                     YDNIALPVTLDKGNHIDHEYIEMLMKTLGIWDKRLKFPSELSGGQQQRVAIARALANK
                     PALILADEPTGNLDSKTTMEVVCLLKESSAKFHQTILMVTHNENIAQVCDSIIHIEDG
                     VVVNTGGEVL"
     gene            2463..5018
                     /locus_tag="PC700_002909"
     CDS             2463..5018
                     /locus_tag="PC700_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2582187.1"
                     /translation="MMSLVKFSIQYIKHYKKQSISIVLSIVLSVALLTGIGSLVHSAD
                     KSRIEKIREDSGDYHYFYKVDKEQLNKIKKNKKSKEYIVDRLGVTKPKNQIDEPFIIE
                     FLNADSSYLDMTGRKLLKGTLPSKDGEIVLDTYSLNNLNVNKEIGTEINLDGKIYKLC
                     GILSSLSNPETAMEGFVNDYLQPKEKDSYIVYVKYDENKNIKKQNYKFMKKFNISKEK
                     TYINWPLSVEFGVKPPKNIEKYNIFEFLKNLELNTNAIILLVGVFGAAAVYSIFHVSI
                     LQRISQYGVLEVLGANNKQLLFLLFLELFLLFIVGFPIGCFLGIGVASTIYEQFPHIF
                     LSSDIVPGAFFISEKSIYLGFLFILLLLILIAVRVVHQLNKYSSIESMKNFKITNKKK
                     RILESVKHSNMTKVLSHKYMTQKRGMFIGILFSLSLGGIIFLCSSYSIQLTKNNNELT
                     MKADDGLNSDYQITMQTTDFDVGIPKDKISRLNNVEGISKVYPVRYLFGAMQIKEEQL
                     FWKNFFKPLEKDYRIKTFFDGICTKQNEGGYLLKTNIYGYNNGMLEGLNSYIIDGEIN
                     THDMVKNNKVIVRLPMDGTGMYDAVDINPGDTIKVKVPKTMKPTGEIVKFKEENKNDY
                     TTKEFVVAATVKRVMANNIYFIGDYGMDIVMTNGQMKNNFDIENYNSVSIKKTEDSNS
                     EELSENIKSVVSDVKRCIVTDYTIAIEKNNTYLNQKLLFIYGIVFVLLAISLFHIINT
                     VSYLIFSRRHEFGILRAMGITDNKFLLMMIREGFLYGFYASIIMVIGSVIGQFMIYFM
                     VKRVYLYINPILKINTPLYIGMIILNITISIVAVIIPVRQILKSDIISEINKS"
     gene            complement(5212..6410)
                     /locus_tag="PC700_002910"
                     /pseudo
     CDS             complement(5212..6410)
                     /locus_tag="PC700_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493023.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
     gene            6529..7416
                     /locus_tag="PC700_002911"
     CDS             6529..7416
                     /locus_tag="PC700_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2582188.1"
                     /translation="MNIKLELYKVFNAVVNNKSFSLAAKELFMSQPAVSQSIKQLEEQ
                     LDTLLFYRNNKGVKLTPEGKILSEHVTTALKLISSGEDRINKFKKLEYGSLKIGVGDT
                     AARFFLLKYLEIFHKKYPHIHVSTINRTSRELISLLKDGNIDIAIINMPIEDDTLNIV
                     ECIEIHDIFVCANDYIEYKGRKISLEELNTLPLIMLENKANSRLYVNEYFLSKGIKLN
                     PDIELGSHELLLEFAYINLGVSCVIEEFSIDYLENEKLFKLDIKEPIPKRNIGYCHLK
                     DISLSLATKEFLSMISNNI"
BASE COUNT         2912 a          886 c         1139 g         2505 t
ORIGIN      
        1 ttttattttg aaaacaaatt taaatacaat ctttttagtg aaataaataa aattaaaata
       61 tttttagtat gataatgcaa gaaaataatt atttatctaa aaaatctaaa ggagagtaaa
      121 attgaactta ttaattattg aagatgatat aaacttaaat gaaggtcttt tttatgcatt
      181 tgagaatgat ggatttaatg tatttaaggc ttatacaaaa caagaaggat taaatatatt
      241 taatagtaaa aatatagatt ttataatctt agactgcaat cttccagatg gagatggatt
      301 tgatgtatgt caaataataa gggaaaagtc agatatacca attataatgc ttactgcaag
      361 agactcagaa attgatgaag taaaaggatt agaaataggg cttgatgatt atattacaaa
      421 accattttct ttatctgtat taaaggcaag agtcaaagta gcaataagaa aaaaatcgaa
      481 taataaagta atatattcca atggaataaa attggaccaa aagttactaa aagtatataa
      541 aaataaagca tgtttggaac ttagctctgt agaatataag cttctaagtt atctaattga
      601 aaataaaggt cagatattat taaaggaaca aattttacac catatttggg acagcgaaga
      661 aaattatgtt gatgataata ttgtatctgt aaatataaga agacttagag taaaagtaga
      721 ggatgaccca tctaatccaa aatatataaa aacagcttat ggaatgggtt atttatggaa
      781 tgaggtagaa taatatgttt atattagcct ttataatatg tacagttgtt atatttatag
      841 tatctactaa aataaatcaa agaagatatg atgaattaaa tttgatgtta aatcaaatat
      901 tggaaggtaa agaagttact tatccagata cgaaggatac aagagcgtca aaaatttctc
      961 accaagtaaa aaaaattaag gatatgatag aaatagaggt agaacagtca aagtcagaaa
     1021 aagaagcgat taaaggttta atatcaaata tgtcacatca attaaaaact ccactttcaa
     1081 atataaccat ttattgtgaa cttctggaga atataaatat ttccacatta cagaagaagg
     1141 agtttttaca aaagatgaaa aatgaaactt ttaagattga ctggttgtta caatccttat
     1201 ttaaaatgac taaattagag gatggagtta tagaatttga agtagaagaa ctactaatta
     1261 aagatactct tattcaaagc atcagtacta tctttaataa atcagaagct aaaaatataa
     1321 ggataaatct cgaaccattt tcagatataa agttagttca taataaaaag tggaccatag
     1381 aagcaattgt aaatgtttta gaaaatgcaa taaaatactc accatcagat agcacgatta
     1441 ctatttctgt aataaaaatg gagctatata cgaagattac tattaaagat gaaggaatag
     1501 gtattgatag tagagaacta aatgatattt ttaaaagatt ttacagaagt aaaaatgtgg
     1561 aaaaccaaaa tggcacaggg attggtctat atcttacaag attaatttta gaaaaagaaa
     1621 atggaaacat tatagtagaa tctaaactag gaagtggatg ttgttttagt atattcttac
     1681 agaattgtaa gagtttaaac taactttgta ggatgagtgt aattatcgtc acttacaata
     1741 ttttatagaa catatatctg tattgaaagg agaaattaaa tgagtatatt agtgacgaat
     1801 aatttagtga aacactatgg aaaaggtgag actaaagtaa aagccttaaa tggagtaagt
     1861 atagaaattg aacgagatac atttactgct atagttggaa cttctggttc tggaaaaagc
     1921 acattgttaa atattatagg tggattagat aatccaacct caggtgatgt aataattaaa
     1981 ggaaagaaca tatctaaaat aggaaaaaaa gatttgacag tatttagaag aagaaatata
     2041 ggatttatat ttcaaaacta cagtttaatg cccgtattaa acacttatga taatatagct
     2101 cttcctgtta cattagataa aggaaaccat atagaccatg aatatattga aatgctgatg
     2161 aagacattgg gaatttggga taagcgttta aaatttccaa gcgaactatc tggtggtcaa
     2221 caacagaggg tagcaattgc tagagctttg gcaaataaac ctgcacttat attagcagat
     2281 gaaccaacag gaaatctcga tagtaaaact acaatggaag tggtatgttt attaaaagaa
     2341 agtagtgcaa aattccatca aacgatactt atggtaacac acaatgaaaa tatagcacaa
     2401 gtatgcgact caattatcca tattgaagat ggagttgtgg tgaatactgg tggtgaagta
     2461 ctatgatgtc tctagtaaaa ttttcaattc aatatataaa gcattataaa aaacaatcta
     2521 tatctatagt tttaagcata gtactatcag tagcattatt gacgggaatt ggttctttag
     2581 ttcatagtgc agacaaaagt agaattgaaa agataaggga agatagtgga gattatcatt
     2641 atttctataa agtagataag gaacaactta ataaaattaa aaaaaataaa aaatcaaagg
     2701 aatacattgt agatagactt ggcgttacaa aacctaaaaa tcagatagat gaacctttta
     2761 ttatagaatt tttaaatgct gattcttcat atttagacat gacaggtaga aaattattaa
     2821 aaggaactct tcctagtaaa gatggagaga tagtacttga cacatattct ctaaacaatt
     2881 taaatgtaaa caaagaaatt ggaacagaaa tcaatttaga tggtaaaatc tataagttat
     2941 gtggtatttt atcaagtctt tcaaacccag aaactgctat ggagggattt gtaaacgatt
     3001 atttgcagcc taaagaaaag gattcttata tagtttatgt aaaatatgat gaaaataaaa
     3061 atattaaaaa gcaaaattat aaatttatga aaaaattcaa catatcaaaa gaaaaaactt
     3121 atataaactg gcctctttca gttgaatttg gagtaaaacc accaaagaat atagaaaaat
     3181 ataatatatt tgagttctta aaaaatttag aactaaatac aaatgcaatt atattgcttg
     3241 ttggagtgtt tggtgcagca gcagtatata gtattttcca tgtttcaata cttcagagaa
     3301 tatcacaata tggggtttta gaagtattag gcgctaataa taaacaactt ttatttctac
     3361 tatttttaga gttattttta ctttttattg ttggatttcc tataggttgc ttcttaggta
     3421 ttggagtagc gtctacaata tatgaacaat ttccacatat atttttgagt agtgatattg
     3481 ttccaggagc attttttata agtgaaaaat ctatttattt aggcttttta tttatcttat
     3541 tgttgttaat tttaattgct gtacgtgttg tacatcagtt gaataaatac tctagtatag
     3601 aatctatgaa aaactttaag attactaata agaaaaagag aatattagaa agtgtaaaac
     3661 actctaacat gacaaaagta ttatctcata aatacatgac tcaaaaaagg ggaatgttta
     3721 ttggaatttt gttttcatta tcacttggtg gaattatatt tctatgttct agttactcta
     3781 tacagcttac taaaaacaat aatgaactta caatgaaagc tgatgatggt ttaaattctg
     3841 actaccaaat aactatgcaa acaacagatt ttgatgtagg tataccaaaa gataaaattt
     3901 caagattaaa caatgttgag ggaatatcaa aagtctatcc agtaagatat ttatttggag
     3961 ctatgcaaat taaagaagaa caactatttt ggaaaaattt ctttaaacct cttgaaaaag
     4021 attacaggat aaagactttt tttgatggta tttgtacaaa gcaaaatgaa ggtggctatc
     4081 ttttaaaaac aaatatttat ggttataaca atggtatgtt agaaggatta aattcttata
     4141 ttatagatgg ggagattaat acccatgata tggttaaaaa taataaggtt atagttagac
     4201 tacctatgga tggaactggg atgtatgatg cagttgatat aaatccagga gatacaataa
     4261 aagttaaagt gccaaagaca atgaaaccaa caggtgaaat tgttaaattt aaagaagaaa
     4321 ataagaatga ttatacaaca aaagaatttg tagttgctgc tactgtaaaa cgtgtgatgg
     4381 caaataacat atatttcatt ggtgattatg gtatggatat agttatgacg aatgggcaaa
     4441 tgaaaaataa ctttgatatc gaaaattata attcagtaag tataaaaaag acagaagata
     4501 gtaacagtga ggaattgtct gagaacataa aaagtgttgt aagtgatgta aaaagatgta
     4561 tcgtaacaga ttataccatt gctattgaaa aaaataatac ctacttaaat caaaaactct
     4621 tatttatata tggtattgta tttgtacttc ttgcaataag tttgttccat ataatcaata
     4681 cagtaagcta cttaattttt tcaagaagac atgagtttgg aattttaaga gctatgggta
     4741 ttacggataa taagttttta cttatgatga taagagaagg ttttttatat ggattctatg
     4801 caagtattat aatggtaatt ggaagtgtta ttggacaatt tatgatttat tttatggtaa
     4861 aacgtgtata tttatatata aatccaattc ttaaaataaa tacacctcta tacataggta
     4921 tgattatttt aaatatcact attagtatag ttgctgtaat tattccagta agacaaattt
     4981 tgaagagtga tattatttca gaaataaata agagttagag ttatataaat ttttaagatt
     5041 atataacttt atgacaatct tgtttaaatt atttaaatat atcttatctg tgaacattaa
     5101 ataaacctct taatagatat aactcttgaa aagtcatatc tattaagagg ttttaataaa
     5161 ttttcatgtt aatatatata atccaaaaaa attaaatctt attaaaacat actattttgt
     5221 atagttatcg aaatatcctt gtatgtaaat aataggagta cccttatctc cacttccaga
     5281 tgttaaatct gctaaagaac ctataaggtc tgttaatctt cttggagtag taccttgaga
     5341 aaccatgttt cctgttaaat catcagattt attatctttt tcaactatgt attttgaaat
     5401 tgcttctttt aactcatcac cagataagtc agcaaaatca ttatctgcta agtattttaa
     5461 ttttacttcg tttggagttc cctctagccc ttttgtataa gcaggagata ctactgggtc
     5521 agctaattcc catattttac ctacagggct ttaaaagctc catctccata aaccattact
     5581 tctacatctt tccctgttat ttcttttata ttaccttgga ttttgtttac aacttcatcg
     5641 caatttatag ggaataattt tactgtttct tctgtagctt tgtttgaacc taaaagtcca
     5701 tactggtcat tgtaaccact accatctaca cttgctgtca ttatatcatc aagtgaatat
     5761 acttttttag ctccattttg tcttaaaatt ctctttgttc tttgtcttgt atgtatatca
     5821 caagttaaaa tacttgttgt atagttaaga atagtttttg gattgtttgc aaatactatt
     5881 tctacctctg caccacaatc cttgattaaa ttcttataat aatcaacata atctacacca
     5941 gtaaatgtat gcttgttata tccaaatagt tctctgtatt tttcttctgt taaaacatct
     6001 gaccaaggat ttattccctt atcatcaagt tcatcaatac ttattaagtg atttccaact
     6061 tcatcagaag ggtagcttaa cattaatacg acttttctac atccttttgc aattcctttt
     6121 aaacatatag caaaacgatt tctacttaaa attgggaata taaccccaac agtatcatct
     6181 ccaaatttat cttttacatc ttttgcaata ttgtcaacgt gagcataatt tccctgtgct
     6241 ctagctacaa cagcttctgt aacggcaatt acatctttgt ccctaacttc aaagttttca
     6301 ctttttgacg catttaaaac agaatcaaca actattttaa ctaagtcatc tccttgtctt
     6361 ataataggtg ctctaacccc tcttgaaaca gttccaacta atctatccat tatctaaaat
     6421 cctcctaaag aattaatatt gtcatattag taccatgtat attataattc aaatataatt
     6481 ataagtaaaa taaataatac taatatttga cataaggagt aattatatat gaatataaaa
     6541 cttgaacttt ataaggtttt taatgcagtt gtaaataata agagtttttc tcttgctgct
     6601 aaggaattgt ttatgtcaca gccagctgta agccagtcaa taaagcaatt agaagaacaa
     6661 ttagatactt tattatttta tagaaacaat aagggagtaa aattaactcc agaggggaaa
     6721 attctaagtg aacatgtcac tactgcatta aagttgatat catcaggaga agatagaata
     6781 aataaattta aaaaattaga atacggttct ctgaaaatag gagtaggaga tacagctgca
     6841 cgttttttct tgttaaaata tttagaaatt tttcataaga agtatccaca tatacatgta
     6901 tctacaataa atagaacgag tagagaactt atatctttac tcaaagatgg gaatatagat
     6961 atagcaatta taaacatgcc aatagaagat gatacactaa atattgttga gtgtattgaa
     7021 atacatgata tatttgtatg tgcaaatgat tacatagaat acaaaggtag gaaaatatca
     7081 ttggaagaat taaacacatt gcctttaata atgctagaaa ataaagcaaa ttcaagactt
     7141 tatgtaaatg aatatttttt atcaaaaggt ataaaactaa acccggatat agaattgggt
     7201 tctcatgaat tacttttaga atttgcgtat ataaatcttg gagtgtcttg tgttatagaa
     7261 gaatttagca tagattattt ggaaaatgag aaattgttta aattagatat aaaagagcca
     7321 atacctaaga gaaatatagg ttactgccat ttaaaagata tatctttatc cttagcaacc
     7381 aaagaatttt taagcatgat atctaataac atataattta gaatacatat ttcacaagta
     7441 at
//
LOCUS       DAMJWP010000180         7438 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.187, whole genome shotgun sequence.
ACCESSION   DAMJWP010000180 DAMJWP010000000
VERSION     DAMJWP010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7438
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.187"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            19..393
                     /locus_tag="PC700_002912"
     CDS             19..393
                     /locus_tag="PC700_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582189.1"
                     /translation="MGNKNKNTKYKDKTRERYNKKVENLNKEKLNKDNKQSKKVDKID
                     DIINKSSIVLCVLLVVFSLLYLFKLVQLNIIMILLVITVAIQQGLVSFGAFRNKNYVK
                     ALFSIFVIVCFLIAFKYVVNPV"
     gene            485..1945
                     /locus_tag="PC700_002913"
     CDS             485..1945
                     /locus_tag="PC700_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDF2582190.1"
                     /translation="MNRCKFAKLIITSMIVLLLTGCSLKKEDNYKLDPENPTSIQIWH
                     YYNGQQKMAFDKLVKEFNDTIGAEKGIIVEAFSQGNVVDLSEKVLDSANKKVGSEDVP
                     NIFAAYSDTAYEMDKLGLLVDMDKYISKEELNEYIDEYVEEGRINSKDEFKLFPIAKS
                     TEILMLNKTDWDKFAKATNSNINDIKTIEGLVETSKKYYEWTDSLTKEPNDGKAFFGR
                     DALANYIIIGSKQLGVEIFSVKDGKVTLNIEEKVMKKLWDNFYVPYINGYFASYGKFR
                     SDDAKTGDILALVGSSSGAAYFPDRVMLNDNESYNIETSVFQAPRFKGEGKYAVQQGA
                     GMAVTKSTKEEEYASVEFLKWFTDKEPNIKFSIASGYLPVKKKTNDLNVIKEVMKKDN
                     LSLSDKVEGSIASSIEQINSCNLYTNKAFDGGTKARQILEYSMLDKAKEDSNKINELV
                     KSGTSKKDAVAQFNNDKNFETWFESFKAELENAIKK"
     gene            1998..4874
                     /locus_tag="PC700_002914"
     CDS             1998..4874
                     /locus_tag="PC700_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2582191.1"
                     /translation="MENNLRKNNSIMKKMIFPLIIVMLIQTSLFCATILWGGTIKKLN
                     DNSFDILNERVINRKNYIQNEMLQRWSNTSETEATINSSVKKILNEKNALIEDVGNNE
                     EINKDIIKSISNDLIYLLRKNSVTGAFIILNDKNINDKPIDSKGEISRTGLYIRDLDP
                     KFNPNDNSDLLIERGSSDIARSFGISMDSYWEPKFSFKSENEQEGDDFFYKPFRAAID
                     NPNVSSSNLGYWGSKFLLTNAGDRLMTYTVPLIYEDGTVYGVLGIDLSVNYLRELLPY
                     SEINGNKKGNYILGVDRNNDMAFENVISNGYSLKEKFWDGLHSNLEQKESYKNIYKIK
                     NKDNECGNGNIYASVQYLDLYNSNTPFENERWALLGIIEEKSLLSFSKRIEKLVIISI
                     AISLLIGLIGIYIASRLFTKPIVLLANKVRNSNPSKPVNLDKINISEIDELSSAIELL
                     SANVADSSSKLSQIIEMVDMPIGAFELYKNAENVFCTKDKKLKWIRMNIFEDELKVLG
                     VVVDVTKEFIEKRKIEYERDFDILTNLLNRRAFHSQIQEKFKNKDELKIAAFIMWDLD
                     NLKYINDTYGHDAGDEYIRCAADILKKFISWNGIVSRLSGDEFYVFIYGYESKDSIRD
                     IINSVKKQMNNTLLKLHDGTEFKIRASAGISWYPDDSTNYDELMRYADFAMYQIKNTI
                     KGEVSEFDINIYNKDSFLLSNREELNKLIDGQLVEYAFQPIVDCKGKVFAYEALMRPK
                     IKTFSSPIDVIRLARSQSKLYQIERITWFKALKSFKKYKEKFGDCKLFLNSIPNNNLS
                     KVDFEEIEDLYGEYLNRVVLEITENDKINEDFIEKKKAYIDHWNIEIALDDFGTGYNG
                     DAVLLYIMPDYVKIDMSIVRGIDKDENRQKILKNLISYSNERNIKVISEGVETKGEME
                     TLINLGVDYMQGYYISKPSFIPEDISDKIKKELKICHENRNDVV"
     gene            4951..7017
                     /locus_tag="PC700_002915"
     CDS             4951..7017
                     /locus_tag="PC700_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDF2582192.1"
                     /translation="MVELIITTNIDIRKLNENQLEAVEHIDGPCMILAGPGSGKTRVI
                     TYRIANMVVNKNIAPTRILAISFTKASSIEMKNRALNLSDDIRLNKVTYGTFHSVFFK
                     ILRYFERYNLDSIFDEKSKRMTIKAILKSLNIENADDDENIGQVINEISYVKNELMDK
                     NEFNSEVLTKDEFLKVYNLYEEQKSKVNKIDFDDMLIRTYYLLLNNKSALEMVRNVYK
                     YILIDEFQDINKVQFEVLKLICSPLNNIFAVGDEDQSIYGFRGARPDFLLEFEQYFNN
                     TKKIILDINYRSKSEIVYTANRLIDKNKNRYEKVIKCSQGDGGSVTYISPHDSEEEAL
                     YIAKEIIDEIQKDYVEYSDFAVIYRTNIQSRALVDVFMDMRIPFVVKDSVITIYDHWA
                     SQDILAYLRIGINPKSNKDWLRIINKPFRYISKDSINMVKDEKDFITALINKCNLHPK
                     QVKTINDLEIDLSYLNTLNPKNAISYIRTSLDYDRYVLDYCSNRKIKTNGLVEILNEL
                     ESSATNFNTVTEFLEHIDRVKTELTENNKNKQTEGVIFTTMHSAKGLEFRNVYIIGVN
                     EGTIPHEKSYDICKEEKKEEQLEEERRLMYVAITRAEEKLCISSTLNKYGKKVDKSLF
                     INDIKSPTKKEIDSIGVGDKVYHKKFHEGEIIKKDGIMFTIKFKDRERILDLKTCLLK
                     NIIYTI"
BASE COUNT         3091 a          686 c         1299 g         2362 t
ORIGIN      
        1 atagaggatg ggaaataaat ggggaataaa aataaaaata cgaaatataa agataaaaca
       61 agagaaagat ataataaaaa agttgagaat ttaaataaag aaaaattaaa taaagataat
      121 aaacagtcta agaaggtaga taagattgat gatattatta ataaatctag tattgtatta
      181 tgtgtacttc tagtggtgtt ttcattactt tacttattta aattggttca acttaatata
      241 ataatgatac ttttagtaat tacagtagct attcaacaag gattggtgtc atttggtgca
      301 tttagaaata aaaattatgt aaaagcttta ttttctatat ttgtaatagt atgtttctta
      361 attgctttta aatatgttgt aaatcctgtt taaatatatc aataaacgtt ctataattaa
      421 tataaatagt gaagttacag ttttaatgct attataaatt ttataatttg aaggagtaga
      481 ttttatgaat aggtgtaagt ttgcaaaatt aattatcaca tcaatgattg ttttattact
      541 tacaggatgt agtttaaaaa aggaagataa ttataagtta gacccagaaa atccgacatc
      601 aatacaaatt tggcattatt ataatggaca gcaaaaaatg gcttttgata agcttgttaa
      661 ggaatttaat gacactatag gtgctgaaaa aggaattatt gttgaagcgt ttagtcaagg
      721 taatgtagtt gatttatcag agaaagtttt agattctgca aataaaaaag ttggctctga
      781 agatgtgcca aatatatttg cagcatactc agatactgca tatgaaatgg ataaattagg
      841 gttactagta gatatggata agtacataag taaagaagaa ttaaatgaat acatagacga
      901 atatgtagaa gaaggcagaa taaattctaa ggatgagttt aaacttttcc caattgcaaa
      961 atcaactgaa attttgatgt taaataaaac tgattgggat aaatttgcaa aagctacaaa
     1021 ttcaaatata aatgatataa aaacaattga aggattagta gagacatcaa aaaaatatta
     1081 tgaatggaca gatagtttaa cgaaagagcc taatgatggt aaagcatttt ttgggagaga
     1141 tgctttagca aattatatta ttataggaag taaacaacta ggagtagaaa ttttttctgt
     1201 taaagatggt aaagttacac taaatattga agaaaaagtt atgaaaaagc tatgggataa
     1261 tttctatgta ccatatataa atggatattt tgcttcttat ggtaagtttc gttctgatga
     1321 tgcaaaaaca ggtgatatat tagctcttgt aggctctagt tcaggagcag catactttcc
     1381 agatagagta atgctaaatg ataatgagag ttataatatt gaaacatctg tttttcaagc
     1441 accacgtttt aaaggagaag gaaaatatgc tgtacaacaa ggtgcaggaa tggctgtaac
     1501 aaaatctact aaagaagaag aatatgcatc agtagaattt ttaaaatggt ttacagataa
     1561 agagcctaat ataaaattct ctattgcttc tggatattta ccagtaaaga aaaaaacgaa
     1621 tgatttgaat gttataaaag aagttatgaa gaaagataat ttgagtttat ctgataaagt
     1681 tgaaggttca atagcctcat ctattgaaca aattaacagt tgtaatttat acactaataa
     1741 agcatttgat ggtggaacta aagctagaca aatacttgaa tattcaatgc ttgataaagc
     1801 taaagaagat tcaaataaaa taaatgaatt ggtaaaaagt ggaacttcta agaaggatgc
     1861 ggtagcacaa tttaataatg ataagaattt tgaaacttgg tttgaaagtt ttaaggctga
     1921 gcttgaaaat gctataaaaa aatagatgaa ttggcagtta gtgtgtagac aagcacaaag
     1981 gtataggtgg aataagtatg gaaaacaatc taagaaaaaa caattcaata atgaaaaaga
     2041 tgatatttcc cctaattatt gttatgctaa ttcaaacaag tttattttgt gccacaattt
     2101 tatggggagg aactattaag aaattaaatg ataattcgtt tgacatctta aatgaaagag
     2161 taattaatcg taaaaattat atacaaaatg aaatgttaca aagatggtca aatacaagtg
     2221 aaacggaagc tacaattaat tcctctgtaa aaaaaatatt aaatgaaaaa aatgccttaa
     2281 ttgaggatgt aggcaacaat gaagaaatta acaaagatat tataaaaagc atatcaaatg
     2341 atttaatata tcttttgaga aaaaactctg ttacaggagc ttttataatt ctaaatgaca
     2401 aaaatattaa cgataagcca atagatagta aaggtgaaat aagtaggact ggattgtata
     2461 taagggattt agaccctaag tttaatccaa atgataattc tgatttgtta attgagagag
     2521 gttcatcaga tatagcaaga agttttggaa tatctatgga cagttattgg gaacctaaat
     2581 tttcatttaa aagtgaaaat gagcaagagg gagatgattt tttttataaa ccatttagag
     2641 ctgcaattga taatccaaac gtatcaagtt ctaatttggg atactgggga agtaagtttt
     2701 tattgacaaa tgcaggtgat aggcttatga catatacagt tccccttatt tatgaagatg
     2761 gaactgtata tggagtactt ggaatagatt taagtgtaaa ttatttaaga gaattacttc
     2821 catactcaga gataaatgga aataaaaaag gtaattatat tttaggtgta gatagaaata
     2881 atgatatggc ctttgaaaat gtaatttcaa atggatactc attaaaagaa aaattttggg
     2941 atggtttaca ttctaattta gagcagaagg aatcgtataa aaatatatat aagattaaaa
     3001 ataaagataa tgaatgtggt aatggcaaca tttatgctag tgtacaatac ttagatttat
     3061 ataactccaa tactccattt gaaaatgaac gttgggctct tttgggtatt attgaagaaa
     3121 aaagtctttt gtcattttca aaaaggatag agaaattagt tataatatct atagcaattt
     3181 ctttattaat aggtctaata ggtatttaca ttgcatcaag attatttaca aaaccaattg
     3241 ttcttttagc caacaaagtt agaaacagta atccatcaaa accagtaaat cttgataaga
     3301 taaatatatc tgaaatagat gaattatctt cagctataga gttactcagt gcaaatgttg
     3361 cagattcttc atcaaaatta tcacagatta ttgaaatggt agatatgcca atcggggctt
     3421 ttgagttata caaaaacgca gagaatgttt tttgtacaaa agataaaaaa cttaagtgga
     3481 taagaatgaa tatctttgaa gatgagctaa aggttcttgg tgttgttgtt gatgtcacaa
     3541 aagaatttat tgagaaaagg aagatagaat atgaaagaga ttttgatatt ttaacgaatc
     3601 ttttaaatag gagagctttt cattcacaga tacaagaaaa atttaagaat aaagatgaac
     3661 ttaagatagc agcatttatt atgtgggatt tagataatct aaaatatatt aatgatacat
     3721 atggacatga tgctggtgat gaatatatta ggtgtgctgc agacatactt aagaagttta
     3781 tatcatggaa tggtatagta tctagacttt caggtgatga gttttatgta tttatatatg
     3841 gatatgaaag taaagatagt ataagagata ttattaattc tgttaagaaa cagatgaata
     3901 ataccttatt aaaacttcat gatggaacag aatttaaaat aagggcatca gcaggaattt
     3961 cctggtaccc tgatgattca accaactatg atgaattaat gagatacgca gattttgcta
     4021 tgtatcaaat taaaaataca atcaaggggg aagttagtga atttgatatt aatatatata
     4081 ataaggattc attcttattg agtaatagag aagaattaaa caagttaatt gatggtcaat
     4141 tagttgagta tgcatttcag cctattgtag attgtaaagg taaagtattt gcatatgagg
     4201 cacttatgag acctaaaata aaaactttta gttcaccaat agatgtaatt agattagctc
     4261 gttcacagtc taaattatat caaattgagc gtattacatg gtttaaagca cttaaatcat
     4321 ttaaaaaata caaagaaaaa tttggcgatt gcaaactgtt cttaaattct attcctaata
     4381 ataatttatc aaaagtagat tttgaggaaa ttgaagattt atatggtgaa tatttaaata
     4441 gagtagtatt agaaataaca gagaatgata agattaatga agattttatt gagaaaaaga
     4501 aagcatatat agaccattgg aatatagaaa ttgctttaga tgactttggg acaggttaca
     4561 acggagatgc tgtactatta tatataatgc cagactatgt aaagattgat atgtctattg
     4621 taagaggtat tgataaagat gaaaacagac agaaaatatt aaaaaatctt atctcatatt
     4681 ctaacgaaag aaatattaaa gtgatatcag aaggtgtaga aacaaaaggt gaaatggaaa
     4741 cactgattaa tcttggagta gattatatgc agggttacta tattagtaaa ccatcattta
     4801 tacctgaaga tataagtgac aaaattaaaa aagagctcaa gatatgtcat gaaaatagga
     4861 atgatgtggt ttaatagagg aaaaattaaa tttattttta tgaactttta gtttataatt
     4921 aaaaagaaaa taatataatt ataaattaag gtggtggagc taataatcac aacaaacata
     4981 gatataagaa agttaaatga aaatcaatta gaagcagttg aacatataga tgggccttgc
     5041 atgatacttg cagggcctgg ttcaggtaag accagagtaa ttacatatag aatagccaat
     5101 atggttgtaa acaaaaatat agcaccaaca agaatcttgg caataagttt taccaaggca
     5161 tcttcaatag aaatgaaaaa tagagctttg aatttaagtg atgatataag acttaataag
     5221 gtaacctatg gtacttttca ctctgtattt tttaaaattc taagatactt tgaaagatac
     5281 aatttagaca gtatatttga tgaaaagtca aagcgaatga caatcaaggc aatcctaaaa
     5341 agtttaaata tagagaatgc tgatgatgat gagaatatag gacaagttat aaatgaaatc
     5401 tcctatgtaa aaaatgaact tatggataaa aatgaattta attcagaagt attaacaaaa
     5461 gatgagtttt taaaagtata caacttatac gaagaacaaa aatctaaagt taacaaaata
     5521 gattttgatg atatgcttat aagaacttat tatttacttt tgaacaataa gtctgcacta
     5581 gaaatggtta gaaatgtgta taaatatata ttgatagatg aatttcaaga tataaataaa
     5641 gtacagtttg aagttttaaa gttaatttgt agcccattaa acaatatatt tgcagttgga
     5701 gatgaagacc aaagtatata tggatttaga ggtgctagac cagatttttt gcttgaattt
     5761 gaacaatact ttaataatac taaaaaaata atattagata taaattatag gtctaaaagt
     5821 gagatagtgt atactgcaaa tagacttatc gacaaaaata aaaatagata tgaaaaagtc
     5881 attaagtgta gtcaggggga tggaggaagt gttacctaca tttctccaca tgattcagag
     5941 gaagaagcct tatatattgc caaggaaata attgacgaaa tacaaaaaga ttatgtagag
     6001 tattcagatt ttgcagtaat ttatagaaca aatatacagt caagagcatt agtagatgta
     6061 tttatggata tgaggattcc atttgtagta aaagactcag taattacaat atatgaccat
     6121 tgggcaagtc aagatatact agcttattta agaataggta ttaatccaaa atcaaataaa
     6181 gattggttga gaattataaa caagcctttt agatatatat ccaaagacag tataaatatg
     6241 gttaaggatg aaaaagactt tataacagca ttaataaata agtgtaatct tcatcctaaa
     6301 caagttaaaa caataaatga tttggaaata gatttaagtt atttaaatac tttaaatcct
     6361 aaaaatgcaa tatcttatat tagaactagt cttgattatg atagatacgt tttagattat
     6421 tgtagcaaca gaaagataaa aactaatgga cttgtagaga tattaaatga acttgagagt
     6481 tcagcaacta attttaatac tgttacagag tttttagagc atattgatag agtaaagact
     6541 gagttaacgg aaaataacaa gaataagcag actgagggtg tcatatttac aactatgcat
     6601 agtgcaaaag gattagaatt tagaaatgtt tatattatag gtgtaaatga aggaacaata
     6661 cctcatgaaa aatcatatga tatttgtaag gaagagaaaa aagaagaaca attagaagaa
     6721 gaaagaagac ttatgtatgt tgctattaca agagctgaag aaaagttatg tataagctct
     6781 actttaaata agtatggtaa gaaagttgat aaatcattat ttattaatga tataaaatct
     6841 ccaacgaaaa aagaaattga cagtattggt gtaggggata aggtatacca taaaaaattt
     6901 catgaaggag aaataattaa aaaagatgga attatgttta caataaagtt taaagataga
     6961 gagcgaatct tggatttgaa aacttgttta ttgaagaata taatttatac tatttaattt
     7021 agaatctaaa tttagataga aatataatgt attatttatt atgtgcaaga caaatagata
     7081 tgtgtaaaat gtattattta acttattaga aaaagaaata aggatttaat atattattta
     7141 tagttgttta gaaaaagcaa caatgtggaa aatattattt agtattatta aagaaaagca
     7201 acaatgtgaa agatattatt tagtgtaatt taagaaaaag caacaatgtg aaagatatta
     7261 tttagtatta ttaaagaaag agcaacagtg tgaaagatac tatttagtat tattaaagaa
     7321 aaagcaacag tgtgaaagat attatttagt gtaatttaag aaaagcaaca atgtgaaaga
     7381 tattatttag tattgtttaa gaaaaagcaa caatgtgaaa gatattattt agtattat
//
LOCUS       DAMJWP010000181         7421 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.188, whole genome shotgun sequence.
ACCESSION   DAMJWP010000181 DAMJWP010000000
VERSION     DAMJWP010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7421
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.188"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(<1..830)
                     /locus_tag="PC700_002916"
     CDS             complement(<1..830)
                     /locus_tag="PC700_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDF2582193.1"
                     /translation="MDFINKMRIRNTTIFIVLIISMILTFLVCVAFGSVKIEFKEVID
                     VVLRGMDNTEHYKIIYNIRIPRVLATMVGGACLAVSGILLQIFFKNPIVEPYILGVSS
                     GSTLSVALMILGGYTLGFESVNSMSIFVAAFIGALIVMSIVILFANKVKNITTLLIIG
                     IMTGYVCSGITNVLQSFANSDKLKEFTMWSMGSFSGFTWEKVEILIIVGILGLLMTVF
                     IIKPLNAFLLGEEYAKSIGINIRFFRVLIVFISSILAAIVSAFAGPIGFIGLAVPQIS
                     "
     gene            complement(902..2032)
                     /locus_tag="PC700_002917"
     CDS             complement(902..2032)
                     /locus_tag="PC700_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2582194.1"
                     /translation="MKFKSNVLKQFFLIAMVIFMVGGLIGCSKSDKENKQASTGKKLE
                     LKYASGFSVEDLEDGIKKVTDGDNRELILIPKKIKIPEKYKNANIVRTPVDNVLIAST
                     TQACSLRTIDELDSIKAVTTEEQYWTIKEIKDLMKDGKIHYVGSNLAPDYEKIVDLNP
                     DLTIVSSGLSEADTKFIDKLKELGLNYVVDNEYKEQNPFGRMEWTSLIAAFYDKEDMA
                     TSELNKTVQKVEEVSKKIKNKAKPKVVWASVYEGSAYIAPKDSYVDNMIKMAGGINAC
                     ASIDSNEGRVSIEQLHEVAKDADIFVYSSSSDYAPNLDSIKSSAPVLADLDVVKNGNL
                     WVFAPDYYQSVDKTDELIVDLVEMFHPGTTGEKIKHYINYDK"
     regulatory      complement(2260..2441)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(2494..3387)
                     /locus_tag="PC700_002918"
     CDS             complement(2494..3387)
                     /locus_tag="PC700_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HDF2582195.1"
                     /translation="MKAEFLTPVVTIFDEEGNLDKEGNKKVYDHLIDGGVDGLVIMGS
                     TGEFFNMSMDMQKELIDLVTSHVNKRVKVFIGTSRMCINESIELSNYAHNSGADGVMI
                     ISPYYFSLDDDSIELFYSEIAKNTEAEIYLYNYPDRTGYDLSPELTLRLARKHKNIVG
                     YKDTVTLMGHTRDLINTMKIEFPEFKVYSGYDENFIHNLMSGGAGCIGGLSNLIPEVC
                     SKWAEAYNNRDMEKVIEIQKYINKAMDFYLITNPFIPAMKKAMNIRGLQISDYMTAPF
                     VKPNEKQVCEIKKLMNKLNIC"
     gene            complement(3467..4369)
                     /locus_tag="PC700_002919"
     CDS             complement(3467..4369)
                     /locus_tag="PC700_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDF2582196.1"
                     /translation="MKFVSFTEGENECKRTGVFSKDELFIIDVNSLNLSRNFKDLNEL
                     IQNVSSEDIIKLKTIINESIFYNYDVFKRERCTVHVPIEKPIHDILCVGVNYKDHLEE
                     TQTHFDDSFEEPQKTVYFTKRVTKAIGPEDEIDGHFEINNQLDYEVELGVVIGKTGVN
                     IRKEEVEDYIFGYTVINDISARKLQKEHVQWFRGKGLDTFTSLGPCILHKSAVPFPIK
                     LKVCSRVNGEERQSSNTGLFISDVSDIVSELSKGMTLEAGDIIATGTPSGVGMGFSPP
                     RYMSSGDVVECEIESIGILKNYIK"
     gene            complement(4419..5579)
                     /locus_tag="PC700_002920"
     CDS             complement(4419..5579)
                     /locus_tag="PC700_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDF2582197.1"
                     /translation="MKFYGYKRPDGRVGIRNHILILPTSVCASDTTRIIASQVQGAVT
                     FNNQNGCSQVHSDQQLTMDVMAGYAANPNVYGVIVVSLGCENCQNDLVVDAIKERTNK
                     PIKTLVIQEEHGTLKTIEKAVRYAREMAQEASLLRKEEFPISELILGTECGGSDPTSG
                     LAANPLIGELSDKLVDLGATSILSETTEFIGAEHILARRAVNEEVKEKILHIVHRYEN
                     SLKLVGEEVREGNPSPGNIAGGLTSLEEKSLGCIHKGGHRQISEVYDYAKQIDKKGLV
                     IMDTPGNDASSVAGMVAGGAQIVVFSTGRGTPSGNPIAPVIKITGNKITFANMEDNMD
                     FDASPVIYGPQTMEELTDDLLNMVVDVANGKQSKAESLGYTEMAIARVCNYV"
     gene            complement(5735..6040)
                     /locus_tag="PC700_002921"
     CDS             complement(5735..6040)
                     /locus_tag="PC700_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDF2582198.1"
                     /translation="MINAVIIDEKDNVAVAIEAIAKGNEISFKLKDKTVKTITALDNI
                     TIYHKLAIKDMAQGDKVTKYGEHIGEANQEIKSGQHVHIHNVRSVREDLDKQVMTNI"
     gene            complement(6138..7112)
                     /locus_tag="PC700_002922"
     CDS             complement(6138..7112)
                     /locus_tag="PC700_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HDF2582199.1"
                     /translation="MIMDFIKKIPAGMMIVPMFIAAFINTFCPDIVQIGSFTTAVFSS
                     VGSATIIGVQLVCLGTQLQFREMPKVLKRGGILLGSKFAIGALIGILIGKLFGMDGVL
                     GLTTLAVISAVTNSNGSIYLSLMTSYGDETDCATMSLLTLNDGPFFTLIALGTSGLAN
                     VPLLSLLAAIVPILVGMIIGNFDKKMKEFLEPGCNLLVPFVGFALGSSINLASILKGG
                     LSGVLLGLISVFVGGIFIVFCDKFIGKRPGYAGWAVATTAGNAIAVPAAVALVDPSWQ
                     PYVATATTQVAAAVVVTAILVPLITSWWAKKYGCPKFPKQNEDGTITA"
BASE COUNT         2358 a         1424 c          799 g         2840 t
ORIGIN      
        1 tttgaaattt gtggcactgc aagtccaata aaccctatag gtcctgcaaa tgctgatact
       61 attgctgcta atatactaga tataaacact atcaaaactc taaaaaatct tatatttatg
      121 ccaatacttt ttgcatactc ttctcctaat aaaaaagcat ttaaaggttt tattatgaat
      181 acagtcatca aaagacctaa tataccaact attataagta tttctacctt ttcccatgta
      241 aatccagaaa aacttcccat agaccacata gtaaattctt ttaatttatc actatttgca
      301 aatgattgta acacatttgt tataccacta catacatatc cggtcataat tcctattatt
      361 aaaagagttg ttatattctt taccttattt gcaaataaaa tgactataga cattacaatt
      421 aaagctccta taaaagctgc tacaaatata ctcattgaat ttacactctc aaatcccaat
      481 gtgtatcctc ccaaaatcat gagagctact gataaagtag aacctgaaga tactcctaat
      541 atatacggtt caactattgg gtttttaaaa aatatctgta ataaaatacc tgatactgct
      601 aggcatgctc cacctaccat ggttgcaagt actctaggga ttcttatatt gtatattatt
      661 ttataatgtt ctgtattatc catacctctt aatacaacat ctattacttc tttaaattca
      721 atttttacag agccaaatgc tacacacact aaaaaagtaa gtatcatact tataattaat
      781 actataaata ttgtcgtatt ccttattctc attttattga taaaatccat ttgtatactc
      841 ctcttagcaa agttatatta actaaaatct ataaaaccta tctttttgtg tcctctctgt
      901 actatttatc ataatttata taatgtttga ttttttcacc tgtagtacct gggtggaaca
      961 tctcaactaa atctactatt aattcgtccg tcttatctac tgattgataa taatctggag
     1021 caaacaccca taggtttccg ttttttacaa catccaaatc agccaataca ggtgcactag
     1081 attttataga atctaaattt ggtgcataat ctgaacttga tgagtataca aatatatctg
     1141 catcttttgc aacttcatgc aattgttcaa tacttactcg cccttcattt gaatctattg
     1201 aagcacatgc atttattcca ccagccattt ttatcatatt atccacatat gaatctttag
     1261 gagctatata agcacttcct tcatatacag atgcccatac aactttaggt ttagctttgt
     1321 tttttatttt tttacttact tcttctacct tttgtacagt tttatttagt tcactagtag
     1381 ccatatcttc tttatcataa aatgcagcta ttaaacttgt ccactccatt cttccaaatg
     1441 gattttgctc tttatactca ttgtctacaa cataattaag ccctaattct tttaatttgt
     1501 caataaattt tgtatcagcc tctgataaac cactactcac tatagttaag tcaggattta
     1561 agtctactat tttttcataa tctggtgcta aatttgaacc aacgtaatgt atttttccat
     1621 ctttcattaa atctttaatt tctttaatag tccagtactg ctcttctgtt gttacggctt
     1681 ttatactatc tagttcatct attgttctca gagaacaagc ctgtgttgta gatgctatta
     1741 atacattgtc cacaggcgtt ctaactatat tagcattttt atatttttca ggaattttaa
     1801 tcttctttgg gattaaaatc agttctcgat tatctccatc agttactttt ttaattccat
     1861 cttctaaatc ttcaacacta aatccactag catattttaa ttctaatttc ttaccagtac
     1921 tagcttgttt attctctttg tcacttttac tacaccctat aagacctcct accataaaaa
     1981 ttaccatagc aattaaaaaa aactgcttta aaacatttga tttaaatttc atctctttca
     2041 ctccaatatc ttatttttta tgagtggcta aaaaacttta ataatatatg ctttttaaag
     2101 tatacttatt aaagttatcc taaagtcatc tttaataagt ataacctttt aataaaataa
     2161 aaaacttctc ctaatttaat aaggagaagt tcatatatca actttacaaa aacgaattga
     2221 tacttcattt tattataaat tacaatctcc accctaaatt gaacttatat aatactgggc
     2281 aggtctcctg actcacggct tttcctccat cacccttccc aaaattttta taaatttcag
     2341 tggattttga tttcgtatcc gtatacagta gcgggggctg tagtgaattt tcatcacttt
     2401 ccctattaaa ctaatatagt accctagtat tattttaatt taatttttat taagataatt
     2461 gtaatttatt gttgacctct ttgcaaggtt gatttaacat atatttaatt tattcatcaa
     2521 ttttttaatt tcacatactt gtttttcatt tggttttaca aatggagcag tcatataatc
     2581 acttatttgt aaccctctga tattcatagc ttttttcatt gctggtataa atggattggt
     2641 tattaagtaa aaatccattg ctttattaat atatttttga atttctataa ccttctccat
     2701 atctctatta ttgtaagctt cagcccattt ggagcaaact tctggaatta aatttgataa
     2761 tccccctata caacctgctc ccccagacat tagattatgt ataaagtttt catcataacc
     2821 actataaacc ttaaattctg ggaactctat tttcatagta tttattaaat ctctggtatg
     2881 tcccatcaaa gttactgtat ccttatatcc aactatgttt ttatgttttc ttgcaagtct
     2941 taatgtaagt tctggactta aatcataacc agttctatct ggataattgt ataaatatat
     3001 ttcagcctct gtattcttag ctatttcact ataaaataac tcaatactat catcatcaag
     3061 agagaaataa taagggctta ttatcataac gccatctgct ccactattat gtgcatagtt
     3121 tgaaagttct atagattcat ttatacacat acgacttgta cctataaaca cttttactct
     3181 cttatttaca tgactagtta ctaaatctat aagttctttt tgcatatcca tagacatatt
     3241 aaagaactct ccagtacttc ccattatgac tagtccatct acacctccat ctataagatg
     3301 gtcgtacact tttttattac cttctttatc taaatttcct tcttcatcaa atattgtaac
     3361 aactggtgtc aaaaattctg ctttcataat ttaaactcct aaaatttatt ttaatatgtt
     3421 cattaataca tcagatttag ctttaatatt aattatatta aaacatttac ttaatgtaat
     3481 tttttaaaat tccaatactc tcaatctcac attcaactac atctccacta ctcatatatc
     3541 gaggaggtga aaatcccatc cctactccag aaggtgttcc agtagctata atgtctccag
     3601 cctctaaagt catacctttt gaaagttctg aaactatgtc tgaaacatct gatatgaata
     3661 atcctgtatt agaactttgt ctttcttcac catttaccct actacatact ttaagtttta
     3721 tgggaaaagg aactgcactt ttatgtaaaa tacatggacc taaagaggta aatgtatcta
     3781 atccttttcc tctaaaccat tgaacatgtt ctttttgcaa ctttctagca gaaatatcat
     3841 ttataacagt gtaaccaaag atataatctt ctacctcttc ttttctaata ttaacacctg
     3901 ttttacctat tactactcca agttcaactt catagtctag ttgattattt atctcaaaat
     3961 gaccatctat ctcatcttct ggacctattg cctttgtcac acgttttgta aaataaaccg
     4021 ttttttgtgg ctcttcaaaa ctatcatcaa agtgagtttg agtttcttca agatggtctt
     4081 tatagttcac tcccacacat aagatatcat gtattggttt ttctatagga acatgtacag
     4141 tacatctctc tcttttaaaa acatcataat tatagaatat actttcattt ataatagttt
     4201 tcaacttaat aatatcttca ctagatacat tttgaattaa ttcatttaaa tccttaaaat
     4261 ttctactgag atttaaagag ttaacatcta taataaataa ttcatcttta gaaaaaactc
     4321 cagttctttt acattcattt tctccttcag taaaacttac aaacttcata atctcaccct
     4381 ttattaaaat tctatgtatt tattttctta cttaaagatt atacatagtt gcatactcta
     4441 gctattgcca tttcagtata tccaagggac tctgcttttg attgttttcc atttgctaca
     4501 tctacaacca tatttaacaa atcatctgtt aattcttcca ttgtttgtgg accatagata
     4561 actggactag catcaaaatc catattatct tccatatttg caaaagttat tttatttcca
     4621 gttattttta ttactggtgc tattggattt cctgatggtg ttcctcttcc tgtactaaat
     4681 accactattt gtgctcctcc tgctaccata ccagcaacag atgaagcatc atttccaggg
     4741 gtatccatta taacaagacc tttcttatca atttgtttgg cataatcata tacttcactt
     4801 atttgtctat gacctccttt atgtatacaa ccaagtgatt tttcttctaa acttgttaaa
     4861 ccaccagcta tatttccagg agatggatta ccttctctaa cttcttctcc aaccaatttt
     4921 aatgaatttt catatctatg tactatatgt aatatttttt cttttacttc ttcatttact
     4981 gctctcctag ctagtatatg ctctgcacct ataaattcag tagtttcact taaaattgat
     5041 gttgctccta aatcgacaag tttatcactt aattcaccta ttaatggatt tgctgctaat
     5101 ccacttgttg ggtcagaccc accacattca gttcctagta taagttcaga tattggaaat
     5161 tcttcttttc taagcaaaga agcttcttgt gccatttctc tagcataacg aacagctttt
     5221 tctatagttt ttaaagttcc atgttcttct tgtattacaa gagtttttat tggcttattt
     5281 gttctctctt ttattgcatc aactaccaaa tcattttggc aattttcgca tcccaaagat
     5341 acaactatta caccatatac atttggattt gctgcataac cagccataac atccatagtt
     5401 aattgttggt ctgaatgtac ttgtgaacat ccattctgat tattaaaggt aactgcccct
     5461 tgaacttgtg atgctattat tctagttgta tctgatgcac acacactcgt aggcaatatt
     5521 aatatgtggt ttcttatacc aactcttcca tctggtcttt tatatccata aaatttcata
     5581 ctgatttcct cctaaaatag tctatatact ttttataatt tttatatatt ataattttta
     5641 ttacactcac taatcaaaca gctaaaatcc tatttaaaaa ctatttgaca ctaaaagcaa
     5701 tgaatacaac ttttataact ctataaatat tattttatat attagtcata acttgtttat
     5761 ccaaatcctc tctaacactt ctaacattat gaatatgtac atgttgacca ctttttattt
     5821 cctgatttgc ttctcctata tgttcaccat atttagtaac tttatcacct tgggccatat
     5881 cttttatagc caatttatga taaatagtta tattatctag tgctgtaatc gtttttactg
     5941 ttttgtcttt taatttaaaa ctaatttcat tacctttagc tattgcttca atagctactg
     6001 caacattatc tttttcatca attatcactg catttatcat tattcttgct ctcctttata
     6061 aataatataa ttttattaat atcctaaaat ctaattaaag aatttattat tttaccaaca
     6121 actcactgta atataaatta agctgttatt gtaccatcct cattttgttt tgggaactta
     6181 ggacatccat acttcttagc ccaccaacta gttataagag gtactaatat tgcagtgaca
     6241 actactgcgg ctgccacttg tgttgtagct gttgcaacat atggttgcca tgatgggtca
     6301 actaatgcaa cagcagcagg tacagcaatt gcatttccag cggttgtagc tacagcccat
     6361 cctgcatatc ctggacgttt tcctataaat ttatcacaga atactataaa tatacctcct
     6421 acaaatacag atattaatcc aagtaatact cctgataaac ctccttttag tatacttgca
     6481 agatttatgc tactaccaag tgcaaaccct acaaaaggta cgagtaaatt acaccctggc
     6541 tctaaaaact ctttcatttt tttgtcaaaa tttcctatta tcataccaac aagtattggc
     6601 actattgcag ctaataatga caacaatggc acatttgcaa gacctgaagt cccaagagct
     6661 attaatgtaa aaaatggtcc atcatttaaa gtaagtaaac tcatagttgc acaatctgtt
     6721 tcatctccat atgaagtcat caatgaaaga tatatactac cattactatt tgtaactgca
     6781 cttataacag ccaatgttgt aagaccaagg acaccatcca ttccaaatag ctttcctata
     6841 agtataccta tcaaagcacc tatagcaaat ttagaaccaa gaagtatacc acctctcttt
     6901 aaaacttttg gcatttctct aaattgaagc tgagttccca agcataccag ttgaacccca
     6961 attatagttg cagacccaac acttgaaaat actgctgtcg taaacgaacc tatttgtaca
     7021 atatctgggc aaaaggtatt aatgaaagct gctataaaca ttggcactat catcatacct
     7081 gcaggaattt ttttaataaa atccattatc ataaagttaa tcctcctaaa acatgatatt
     7141 taattactct tattaagttt ttagctttaa ttctaacggc cgtttcggtt cgtatttaag
     7201 aactaagttt ttaattacga atctttgaat taatgatagc attttcctat ttatgagtca
     7261 ataaaaaaaa taaatttttg tcaaattttt ttcaaacttt ctgataatac cttgtatatt
     7321 atcaaatgac ttccttatat gcaaaaatac agcgtattta aaattatata gtattcaaaa
     7381 ctataaagtt tttatacagt gtaaaaaata gataaaatat a
//
LOCUS       DAMJWP010000182         7360 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.189, whole genome shotgun sequence.
ACCESSION   DAMJWP010000182 DAMJWP010000000
VERSION     DAMJWP010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7360
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.189"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(62..1009)
                     /locus_tag="PC700_002923"
     CDS             complement(62..1009)
                     /locus_tag="PC700_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDF2582200.1"
                     /translation="MFYFLKVKVGDIMDEVKEFLVLEEEEEVRLDIYLAEQLGDMSRS
                     YIQKIIKDGKVKVNNKVEKAKYLVKEEDNIVIEIPEPKVLEVLPQDIPIDIVYEDDDV
                     LIINKPQDMVVHPAPGNYENTLVNGILYHCKDKLSSINGVIRPGIVHRIDKDTSGLLM
                     IAKNNYAHNFLAEQLKEHTITREYEFICYGVVKEDKITVDKPIGRNPKDRLKMAVVKD
                     GKNAITHFEVVERFDKFTHMRARLETGRTHQIRVHALSINHPLLGDFVYGPKETKFKL
                     KGQTLHAKKLGFIHPTTKEYIEFNSELPEYFKEAIKKIK"
     gene            complement(978..1424)
                     /gene="lspA"
                     /locus_tag="PC700_002924"
     CDS             complement(978..1424)
                     /gene="lspA"
                     /locus_tag="PC700_002924"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDF2582201.1"
                     /translation="MLYILIIILLIGLDQLSKIWVLNNLVDVSTIPIINNVFHLTYVE
                     NRGAAFGLLQNNQWIFIIVALLATVFGLYYLNTRKVHIFGRLGIILIISGALGNLIDR
                     VRLGFVVDYFDFRVIWEYVFNVADVFVVVGTVFLCIYVLFFESKSR"
     gene            complement(1734..2513)
                     /locus_tag="PC700_002925"
     CDS             complement(1734..2513)
                     /locus_tag="PC700_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2582202.1"
                     /translation="MKKAIWGICICLLSIVGVYTYNHNTHNQNSSKETYTLNDNIKDD
                     YEDVIIKKGNEDEKILALTFDDGPDEDFTPQILDILKKNNVKATFFVVGEKVEYNKEL
                     LKRQYDEGHEIGNHTFTHINVAKNSYGSVEKEITDTQNIIKSVIGVEPKIFRPPYRAM
                     SKSVCDIIVSKGMNIILWSNLDPRDWSNPGVDTIINTILTKVQNGNIILLHDYNTKRN
                     DKSQTIQALEVVIPKLKERGYKFVTVSELIQHLDKNKQVQK"
     gene            complement(2563..3045)
                     /locus_tag="PC700_002926"
     CDS             complement(2563..3045)
                     /locus_tag="PC700_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HDF2582203.1"
                     /translation="MDTKKYKDKLLKEREKLTNLVVDMKDNTLFGDTTKHTSEKYSSG
                     ELSSYDNHIGDMGTDLYMQNMQNSLINHEEGRLYQIDSALSKIENGTYGFCDLCHNQI
                     DLERLDILPDTNLCNNCAKKENALLGDAMQNPDVEDSNFYSEDLTNLTDLNKNGTLRD
                     "
     gene            complement(3229..4650)
                     /locus_tag="PC700_002927"
     CDS             complement(3229..4650)
                     /locus_tag="PC700_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HDF2582204.1"
                     /translation="MVSFLGSIVVLIVGYFIYGTFVEKVFGINDKNQTPAIACRDGVD
                     YVPMKWKRIFLIQFLNIAGLGPIFGAIQGALFGPSAFLWIVFGTIFAGGVHDFASGYL
                     SMKNKGTSASELVGLYLGNGAKIAMRIFSVILLVLVGVVFVTGPAGLLKTLTGVNAQI
                     WVGVIILYYIMATVLPIDKLIGKIYPLFGAALLIMAVGVAGGLIIKGYNIPNINLQNM
                     NPNGTPLFPYLFITIACGAISGFHATQSPLMARCVEKESEARPVFYGSMVAEGIIALV
                     WAAAAMSYFHGIPQLNVIFNDGGAATVVNTVSVGLMGPIGGALAILGVVACPITSGDT
                     AFRSARLTIADAMNYNQDAVKNRFLIALPLFAVGVALTFIDFNIIWRYFSWANQTLAM
                     IMLWTGSAYLVKANKNHYITTLPALFMTVVTFSYIMQAKEGFRLPVNISNGIGIVVAI
                     ILGLLFFKKAKDIKEQNAHKLAS"
     gene            complement(4796..5566)
                     /locus_tag="PC700_002928"
     CDS             complement(4796..5566)
                     /locus_tag="PC700_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2582205.1"
                     /translation="MRAIIVEDEFPARKELRYFIENKSSIEVVNEFTNGIEVLDFIQE
                     NKIDVIFLDINIPHLDGMLLAKTLNQFKSRPKIVFITAYENYAVDAFSLDVFDYVLKP
                     YSEERIISMLNKLEKSEMSDIELSNSNSNLYKYKKETVNQEIEEITHKISLWKGDKLV
                     VIDIDDIYYCEANERQTFIYTEKEKFILKEGISEVENLINDKTFFRTHRSYIVNLTKV
                     KEIIPWFNNTYILKLKNSDYEVTVSRSKVKEFRLLMHI"
     gene            complement(5571..6773)
                     /locus_tag="PC700_002929"
     CDS             complement(5571..6773)
                     /locus_tag="PC700_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF2582206.1"
                     /translation="MKTSILIALIEKTSLIVVLFLLITKLKIFKQIFQKEEYSFNDLV
                     CIALVFTFLAIFGTYSGINYMGSIVNTRIISIVSGGILFGPMVGITAGVFSGIHRYFM
                     DIGGITSVPCLLSSILAGVLSGFLYKRIPKQHRVMYGILVGMISESFTILLIYLISYP
                     HSLAIQIIVGIYLPLIVGQIGIGFVISIVEGIEKDKKDIEARNKAEIKALQRQINPHF
                     LFNSLNTIASFIRFSPDKARELIINLSTYLRYNLEYSDNLIDINKEIEQVKSFVEIEK
                     ARFGELLTVSYDIEDVNIKIPSLIIQPLVENAIIHGILESGRAGVVKISIKKLPYSLE
                     NTVRISIEDNGIGISEEIINNVYQDNMPENKIGLYNVHLRLKLMYGRGLNIRRTDNGT
                     LIVFYVKE"
BASE COUNT         2462 a         1286 c          733 g         2879 t
ORIGIN      
        1 ttcactaaat ttatttactt atataaaaac aaaaaatgat ttataagtat tatacaaatg
       61 gttattttat cttcttaata gcttccttaa aatactcagg taattctgaa ttaaattcga
      121 tatattcctt agtggtagga tgtataaatc ccaacttctt agcgtgcaaa gtttgtcctt
      181 ttaatttaaa tttagtttct tttggaccat atacaaaatc acctaaaagt gggtgattta
      241 tagacaaagc atgaactctt atttgatgag ttctaccagt ttcaagccta gctctcatat
      301 gtgtaaactt atcaaatctt tctacaactt caaaatgagt aattgcattt tttccatcct
      361 ttactacagc catttttaat ctatccttag gatttctccc tataggttta tcaacagtta
      421 ttttatcttc tttaaccaca ccatagcata taaattcata ctctctagtt atagtatgtt
      481 cctttaactg ttctgctaga aaattatgtg cataattgtt cttagctatc ataagaagac
      541 cagaagtatc tttgtctatt ctatgaacta taccaggtct aataactcca tttattgacg
      601 acaacttatc tttacaatga tataaaatcc catttactag tgtattttca tagtttccag
      661 gagcgggatg aacaaccata tcctggggtt tatttattat taagacatca tcatcttcat
      721 atacaatatc tattggaata tcttgaggta aaacctctaa tactttaggt tctggtatct
      781 ctataacaat attatcttcc tctttaacta gatatttagc tttttctact ttattattga
      841 cctttacttt tccatctttt ataatttttt gtatataact tcttgacata tcacctaatt
      901 gttctgctag ataaatatca agtctaactt cttcttcctc ttcaagtact aaaaactctt
      961 ttacttcatc cattatatca cctactttta ctttcaaaaa ataaaacata tatacataaa
     1021 aacacagttc ctacaactac aaatacatca gctacattga atacatattc ccatataact
     1081 ctaaagtcga agtaatctac cacaaaacct aatcgtactc tatcaattag atttcctaat
     1141 gcaccagata taattaatat aattcccaac cttccaaata tatgtacttt ccttgtatta
     1201 agatagtata atccaaatac tgttgcaagt aatgcaacaa ttataaatat ccactgatta
     1261 ttttgtaata atccaaatgc tgcacctcta ttttcgacat aagttaaatg aaatacatta
     1321 tttattattg gtattgttga aacatctacc aaattattca atacccatat tttagacagt
     1381 tggtctaagc ctatgagtag aattattatt aatatatata gcaatgattt ttccccctat
     1441 aaattattta aaaaccaatt ttatttgaaa cacactatta ttattataca aaaaaaatgc
     1501 tctaaagagc aactttatta aaattgtatt ttcttaataa agctagagca aaatttaaaa
     1561 tagtaccatc ttttttatat tgcagattgc tacatattta aaatcatatt attatgtata
     1621 aaaaatttaa aaataacata ctaattaata ctttttgtgt gttttataga ttaaagatta
     1681 atgatgtctg tactttaaac ttacaagcac cactaatctt caaacaaata tttttacttt
     1741 tgcacttgct tatttttatc taaatgttgt ataagttcag atactgttac gaatttatat
     1801 cctctctctt ttaatttagg aattactact tctaaagctt gtatagtttg tgatttatca
     1861 tttcttttag tgttataatc atgtaaaagt attatattac cattttggac ctttgtaaga
     1921 atagtattta taattgtatc tacaccaggg ttagaccaat ctctagggtc taagtttgac
     1981 caaagaatta tattcatgcc tttagaaact ataatatcac agacactttt actcatggct
     2041 ctatatggag gtctaaatat ctttggttct actcctatta cactttttat aatattttgt
     2101 gtatctgtta tttctttttc aacactacca taactatttt ttgcaacatt tatatgtgta
     2161 aatgtatgat tccctatttc atgcccctca tcatattgcc tttttaataa ttctttgtta
     2221 tattctactt tttctccaac tacaaaaaaa gttgctttta cattattttt ctttaatata
     2281 tcaagtattt gtggtgtaaa atcctcatct ggaccatcat caaaagtcaa tgctagaatc
     2341 ttttcatcct catttccttt ttttattatt acatcttcat aatcgtcttt tatattgtca
     2401 tttaaagtat aggtttcttt tgaactattt tgattatgtg tattatggtt atatgtatat
     2461 acacctacta tggataataa acatatacat ataccccata ttgctttctt catgtcatta
     2521 cctcttttct aattcattct aacaatatat gatgtcaggc ttctaatctc tcaatgttcc
     2581 atttttattt aaatcagtta aatttgttag gtcttctgaa taaaagttag aatcttctac
     2641 atcaggattt tgcattgcat cacctaataa agcattttct ttttttgcac aattgttaca
     2701 taaatttgta tcaggtaata tatctaatct ttctaaatct atttgattgt gacataaatc
     2761 acaaaaccca tatgttccat tttctatttt agataatgct gaatctattt gatacagtct
     2821 tccttcttca tgatttatta aactattttg catattttgc atatataaat ccgttcccat
     2881 atctccaata tgattgtcat aactagataa ttctcctgat gaatattttt cactagtatg
     2941 tttagtagtg tctccaaaca aggtattatc tttcatatct acgactaaat tagttaactt
     3001 ttctctttct tttaataatt tatctttgta ttttttagta tccattttca acacctctaa
     3061 ttttaatatt tatatcataa aaatagtatg tggaaaacta aattaaaaat acaataaaaa
     3121 acaagatttg attttttcaa aaaaaattgt aggtaaactt aaaattcacc tacaaattct
     3181 ttacatatat ttttaatata atgtttttag tttttgataa taattacatt aacttgctaa
     3241 tttatgagca ttttgctctt ttatatcctt agctttttta aaaaataaaa gtccaaggat
     3301 tattgcaact acaataccta ttccattaga tatattaact ggaagtctaa atccttcttt
     3361 tgcctgcatt atataactaa atgttactac agtcataaaa agtgctggca atgtagttat
     3421 gtaatgattt ttatttgctt tgactaaata tgcagaacca gtccacaaca ttatcatagc
     3481 aagtgtttga tttgcccaag agaaatatct ccaaattata ttaaaatcta taaatgtaag
     3541 ggcaacccct acagcaaata atggcaaagc tattaaaaat ctatttttaa ctgcatcttg
     3601 attataattc attgcatcag ctattgttaa tctagcactt ctaaatgctg tatcccctga
     3661 agttatagga catgctacaa caccaagtat ggccaaagcg cctccaatag gtcccattaa
     3721 tccaacagat actgtattta caacagttgc tgctccacca tcattaaata ttacattaag
     3781 ctgaggtata ccatggaaat atgacatagc tgctgctgcc caaactaaag ctattatacc
     3841 ttctgcaacc attgacccat aaaatactgg tcttgcctca ctctctttct caacacatct
     3901 cgccataagt ggtgattgag ttgcatggaa acctgatatt gctccacatg ctattgttat
     3961 aaataagtat gggaataaag gtgttccatt tggattcata ttttgtaaat ttatatttgg
     4021 tatgttatac ccctttatta ttaatcctcc agctacacct actgccataa ttaataatgc
     4081 tgctccaaat aaaggatata ttttaccaat caatttatct attggtaaaa cagtagccat
     4141 aatataataa agtattatca ctccaaccca tatttgtgcg tttacacctg tcaatgtctt
     4201 taataatcct gctggacctg ttacaaatac aactccaacc aatactaaaa gaataacact
     4261 aaatattctc attgctattt ttgcaccatt tcctagataa agacctacca actctgatgc
     4321 agatgtacct ttgttcttca ttgataaata tcctgaagca aaatcgtgta ctccacctgc
     4381 aaagattgtt ccaaacacaa tccacaaaaa tgctgatggt ccaaataacg ccccttgtat
     4441 agctccaaat atcggaccta accctgctat atttagaaac tgaattaaaa agattctctt
     4501 ccatttcatt ggcacataat ctactccatc cctacaagct atagctggag tttggttttt
     4561 gtcattgatt ccaaaaacct tttctacaaa agtaccatag ataaaataac ctactataag
     4621 tactactatt gaacctaaaa agcttaccat aaattcatcc ccttagtttt tatataattg
     4681 ctttataaat ttttatgagt ttaagaaaac tatttccagt tcaaatagta atactttttt
     4741 atgctaatat aaatattaat tacattaagt gcatatttaa gtacatgaaa tgactttata
     4801 tatgcattaa cagcctaaat tcttttactt tgcttctgct tacagttact tcatagtcac
     4861 tattttttaa tttcaatatg tatgtattgt taaaccatgg tataatttct tttactttgg
     4921 ttaaatttac gatataagac ctatgtgttc taaaaaatgt tttatcattt ataagattct
     4981 ctacttctga tatgccttct tttaatataa acttttcttt ctcagtatat ataaatgtct
     5041 gcctttcatt agcttcacaa taatatatat catcaatatc tattacaacc aatttatcac
     5101 ctttccaaag gctaatttta tgtgttattt cttctatctc ctgatttact gtctcttttt
     5161 tatatttata taaattacta ttgctattag acagttcaat atcactcatc tcactttttt
     5221 ctaatttatt caacatagaa ataattctct cttcagaata tggcttcagt acataatcaa
     5281 atacatctaa agaaaaagca tctactgcat aattttcata cgctgttata aaaacaattt
     5341 taggccttga tttaaactga tttaatgttt ttgcaagaag cattccatcg agatgaggta
     5401 tatttatatc caaaaaaatt acatctattt tattttcctg aataaagtct aacacttcta
     5461 taccattagt aaattcattt actacctcta tgctactttt attctcaata aaatatctta
     5521 attcctttct tgccggaaat tcatcttcca ctattatagc tctcatactt ctactccttt
     5581 acataaaata ctatcaaagt cccattgtct gttcttctta tattaagccc tcttccatac
     5641 atcagtttaa gccttaaatg tacattataa agtccaatct tattttctgg catattatct
     5701 tgatatacat tatttataat ttcctcactt atacctatac cattatcctc tattgatatt
     5761 ctcacagtat tttctaatga ataaggtaac ttttttatgg atattttcac tacaccagct
     5821 ctaccacttt ctaaaatacc atgtattatc gcattttcca ctaatggttg tataatcaaa
     5881 cttggaatct ttatatttac atcttctata tcataagata cagttagtaa ttctccaaac
     5941 ctagcttttt ctatctctac aaaagattta acttgttcaa tctctttatt tatatcaatt
     6001 aagttatcac tatattcaag attatatctt agataagttg ataaatttat tattaactct
     6061 ctagccttat ctggactaaa tcttataaat gaagctattg tgtttaggga attaaacaaa
     6121 aaatgcggat taatttgtct ttgaagtgct tttatttctg ctttatttct cgcttctata
     6181 tcttttttat ctttctcaat cccctctact atagatataa caaatcctat tccaatctgt
     6241 ccaacaatta aaggtaaata tatcccaaca attatttgaa ttgctaaaga atgtggatat
     6301 gatattaagt atataagtag tatagtaaaa ctttcagaaa tcatacctac caatatacca
     6361 tacattactc tatgttgctt tggtatcctc ttatatagaa atcctgataa tacacctgct
     6421 agtatacttg ataaaagaca tggaacagat gttatccccc caatatccat aaaatatcta
     6481 tgaatccctg aaaaaactcc tgctgtaata cctaccattg gaccaaataa aatgccacct
     6541 gatactatag atattattct agtgtttact attgacccca tataatttat accactataa
     6601 gttccaaata tggctaaaaa agtaaaaact aatgctatac agaccaagtc attaaaacta
     6661 tattcttcct tttgaaatat ctgcttaaat atttttaact ttgttattag aagaaacagt
     6721 actacaatga ggcttgtctt ttctattaat gcgattaaaa ttgatgtttt catattccac
     6781 ctttaaaaaa taaattttta cacactctaa atttattatg tttgttgtaa cttatatatt
     6841 tgaagtaatc ctatctcgac attttccaca tttaaccttt taattaaata aaaaatggat
     6901 atacttctat taacaactaa tactagctga ctaacacaag cttgtaataa cttgtagtta
     6961 tcattaactc tacaattcta gtatttaatt ttatacttta gaaatctctt ttcttagaag
     7021 taatactttc gaatattata taatacaatg ttatgtagat tcttactcat acaacatatt
     7081 tctttaaaaa ttttatacta ttctttgttc aaatatctca tttgatttaa ttgtgctaaa
     7141 cacacattat catctctctg ttatatattt attatatagc cgtatatcga taaagtaaat
     7201 aaatcctata tttaaaatca tatgtttgat agtaaatcat tataatgtat atttaccata
     7261 ataatttgcc aaccttgaaa gtcacttcta taattagaag ttaaataatt cttctaaaaa
     7321 aaatacctct aaattttatt ttctgaaata catagttata
//
LOCUS       DAMJWP010000183         7239 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.190, whole genome shotgun sequence.
ACCESSION   DAMJWP010000183 DAMJWP010000000
VERSION     DAMJWP010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7239
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.190"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(<1..259)
                     /locus_tag="PC700_002930"
     CDS             complement(<1..259)
                     /locus_tag="PC700_002930"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HDF2582207.1"
                     /translation="MVFETFDKVIKLHPNAKPVFHSDRGFQYTSKVFESKLLAQGMIQ
                     SMSRAGRCIDNAPMEGIWGILKVEMYYLRKFDTYEQLVKIIE"
     gene            355..848
                     /locus_tag="PC700_002931"
                     /pseudo
     CDS             355..848
                     /locus_tag="PC700_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429361.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            1390..2745
                     /locus_tag="PC700_002932"
     CDS             1390..2745
                     /locus_tag="PC700_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2582208.1"
                     /translation="MDTKISINENMSLGKRFFKYLAPSVVAMWVFSLYTMVDGIFVSK
                     GVGELALAAVNISMPFINFIFAVSLLFSTGASTIIAIYLGKKDIKSANEVFSFNLVSI
                     IILSIIILAITFFNLDRLALFLGATESTIGMVKDYLGIIIFFNGFFIVSYSLEVIIKT
                     DGFPILATVGVIISALTNIILDYLFVIEFGWGVKGAGIATVLSQVFSTIFFLIHFLRK
                     NSTLNFSKFRIDFKTLRKIVFIGFPDSTTELSCGIVVLLFNLSLTKYIGENALIYYSV
                     INYINTLVLMTMMGITQGMQPLTSFYYGAGNIDNVKKLLKMGIKATIIASVAVFAICM
                     AFSGPIVSLFIHPEETMLFNEGVRVFKIFSISFLLVGINVIISGFFVSVEKPSISTVI
                     SLGRGLVIVVLSLISMILIFGGQGIWMTTIVSEFICLILSLVFLKKNFSTLDSNLNKV
                     A"
     gene            complement(2863..3531)
                     /locus_tag="PC700_002933"
     CDS             complement(2863..3531)
                     /locus_tag="PC700_002933"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HDF2582209.1"
                     /translation="MKEVLKLADEKFVMSFSGGKDSILALNRMLKKGYKPVALLTTIS
                     EEHGKSWTHNLEYNMLKQVSSNIGLPLLVAECGVEGYEESFERALIKAKNMGATICAY
                     GDIDIESHRKWDTDRCEAVGMKVDLPLWQESREDLVYEFIDSGFCSVVTKVNLKHLGE
                     EFLGKKLTRELVEKIKNAGADPCGEHGEYHTFVVDGPIFKKPVEYEVKGTLIKDGYGY
                     LEIK"
     gene            complement(3598..4026)
                     /locus_tag="PC700_002934"
     CDS             complement(3598..4026)
                     /locus_tag="PC700_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDF2582210.1"
                     /translation="MTKLWAIIFALLAGGSTALEAFINGELGKSTTALVATFISLTVG
                     AIFFLASIIVTGDLKCLVSINGFNYKLLLGGVFGGLIIYFTVKAIPNLGVSNTLILTL
                     VSQVLVGFFIDSVILGQETMHLYKYIGVILLILGTFFIVN"
     gene            4133..4726
                     /locus_tag="PC700_002935"
     CDS             4133..4726
                     /locus_tag="PC700_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDF2582211.1"
                     /translation="MNFCYNCSIDFGGDFMYTNFNIDFNNFNKKENATKFMLMGVLLI
                     ILGLLCLTFKTLGIKLISWTFGIALLFFAYLNLKNINELKRYATKEEIKPSINIQWVL
                     IIACILLFVFPQKIQSIFSLFLGFYLIFNQLVALVNSKNNPYSKFTTWNIVKILFGIC
                     LILSPLFLSRFIVSIMSFFIILFGLVLFFSGNTARKY"
     gene            4846..5781
                     /locus_tag="PC700_002936"
     CDS             4846..5781
                     /locus_tag="PC700_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893027.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDF2582212.1"
                     /translation="MDKFKYAFDDKRYHTWNYYLRNTFGEKVFKVSINAGFTCPNIDG
                     SLGYGGCTYCSKEGSGDFAGNPKDNLISQFYNIKEMMLKKWPHAKYIGYFQAYTNTYA
                     PLEVLKEKYETILELEDVIGLSISTRPDCLPDDVVEYLSELNKRTNLWVELGLQTIHD
                     STSKIINRGHDYKTFVEGVEKLKSKNIKVVVHIINGLPGEDYNMMMETAKAVGKMGVD
                     GIKIHLLHVIKDTPMEKMLQNGMLTLMEQDEYINLVCDQLEILPETMIIHRLTGDGKR
                     DELVGPIWSLKKWEILNQIDDTLKARNSYQGCKFV"
     gene            complement(6052..6996)
                     /locus_tag="PC700_002937"
     CDS             complement(6052..6996)
                     /locus_tag="PC700_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDF2582213.1"
                     /translation="MGEKSKGYVFIAIAGLLWATLGLFGKFLMGNGLTSEQVAFTRLF
                     FGFIVLGVYSSIRTPQILKISKKGIIYSVIIGIICQAMFNLCYFKAIDIAGVSIAAVL
                     LYTSPLFLAIFSKVCYKENITRSKLFSLILCFIGAIMAVTGGRLDFQGLNAFGLLLGV
                     LSAIAYALMPTISKNALKEFSSSTILVYSFLFGAIFMIPSSRPWEILNYAKDLDVLSC
                     MLMLGIVPAALAYIFYAAGISKGVELSVAGVVASVELVGSVIIGCTILGESFSLGKLF
                     GVMLMLISAVVALNLSYDEIRIFYKSNKLKQIEKTESI"
BASE COUNT         2580 a         1063 c          896 g         2700 t
ORIGIN      
        1 tctctattat tttaactaat tgttcatatg tatcaaattt ccttaaatag tacatttcca
       61 cctttaaaat tccccaaatt ccttccatag gagcattgtc aatacaacga ccagctcttg
      121 acatactttg tatcatacct tgtgctaaaa gtttcgattc aaacacctta ctagtatact
      181 gaaaacctct atcactatga aaaacaggtt ttgcatttgg atgtagtttt attactttat
      241 caaaagtttc gaataccaag ttattattat tcgattttcc aataacatat gaaattaata
      301 gatataacgc cttctatttt acagtcttat ataaataaca tatacaatga gcacatgtta
      361 aattcaataa aaaaccaagt cgattttata aaatcagtat tgaaagaatc ttatagatta
      421 aaagaaatat cagaaaatat ttgtgatttt gttacaaccc caatcaaaaa aaatagttca
      481 acttctaagt tttatacaaa gcaagaaact caattattat tagaaaaatc aaaaaatatt
      541 cctataggta taccaatttt tttgatacta actcttggat tacgttttgg tgaagctgta
      601 tcacttatat ggtcagatgt agaccttgat aaaaaatctc acatgtaaat caaacattag
      661 tttatgtata caataaaata atcttcaaag accctaaaat ccctagatcc aaaagaaaat
      721 tatttgctcc tgatgaactt ataagtttgc ttaaagggca aaaattaaaa caaaataagt
      781 taaaattaca gggtatattg aaaaatgaat ttgatttgat atgcttaaat aaaagattca
      841 acccgtgagt tcaaccaact tttgttgttc attttaaaaa actattaaga gataataatc
      901 ctagagatat aaaagtacat gaattaatac atgcaaatgc gactttgata ttactttcag
      961 gtactaacat aaaagctatt tataaaatat tagtacatgc agatataaaa atagctatga
     1021 ataaatattc gatattctca cttattagat gaaatggata aagaaacatc tgaaaatata
     1081 agcaaaatac tattcaaata atgttcagtt ttatgtcaat taaatgtcac tcaaagtgct
     1141 ttttttccat ttgtcagtta aatgtcactt aaagtacctt tttcctattt gtcagttaaa
     1201 tgtcagtaat atataaataa gatatattta taagaccctt aaaatcacta ttatttaata
     1261 aaatttataa ttatatacta tttgctgttg acattggtat tataccatag tttatcattt
     1321 atattagaaa taaaattatt aattaatagt ttcgtaattt tataatagat ttacagggag
     1381 tgataataaa tggatacaaa aatatctata aacgaaaaca tgagtctagg taaaagattt
     1441 tttaaatacc tagcaccatc tgtagttgca atgtgggttt tctcactata tacaatggtt
     1501 gatggaatat ttgtaagtaa aggagttgga gaattggctt tagctgctgt caatatatca
     1561 atgcctttta ttaattttat atttgcagtt tctctattat tttcaactgg tgcatctacc
     1621 ataattgcaa tctatctggg gaaaaaagat attaaatctg ctaacgaagt cttttcattt
     1681 aacttagtat caataattat attatcaata attatattag ctataacttt ttttaattta
     1741 gatagattag cactattttt aggagctaca gaatctacaa ttggtatggt taaagattat
     1801 ttaggaataa taatattttt taacggattc tttatagtgt cttattcttt agaagttata
     1861 ataaaaactg atggctttcc aatattagct actgttggtg taataatatc tgctcttaca
     1921 aacatcatac ttgattactt gtttgtaatt gaatttggat ggggtgtaaa aggtgctggt
     1981 attgcaactg ttttgtctca agtcttttct actattttct tcttaataca ttttttaaga
     2041 aaaaactcta ctttgaattt ttcaaaattt agaattgatt ttaagacact tagaaaaatt
     2101 gtatttatag gttttcctga ttcaactact gaattaagtt gtggtatagt tgttttactt
     2161 tttaacttaa gtcttactaa atatattgga gaaaatgctc ttatttatta tagtgtcata
     2221 aattatataa atacattagt acttatgact atgatgggta taactcaagg tatgcaacca
     2281 cttactagct tttattatgg tgcaggaaat atagataatg taaaaaaatt attaaaaatg
     2341 gggataaaag ccacaataat agcatcagtt gctgtatttg caatttgtat ggctttttca
     2401 ggaccaatag tctctttatt tatacaccca gaagaaacaa tgctattcaa tgaaggagtt
     2461 cgtgtattta aaatattctc tatatctttc ttattggtag gaattaatgt aataatttct
     2521 ggattctttg tatcagtaga aaaaccatca atttctactg ttatatctct tggtagaggg
     2581 cttgtaatag ttgttttaag tctaatttca atgatactaa tatttggtgg acaaggaata
     2641 tggatgacta ctattgtttc tgagtttata tgtcttattt tatcattagt ttttctaaaa
     2701 aagaattttt ctacattaga ctctaatcta aataaggttg cttagatgat aagagtagca
     2761 atatatgaaa atatctaaat aaaaaatacc tattatacaa tcttgcaaat attagacctt
     2821 tgcaaaaatt aggattatat aataggtatt ttatttatgt aattatttta tttctaaata
     2881 tccatagcca tcttttatta aagttccttt tacttcatat tctacaggtt ttttaaatat
     2941 tggaccatct acgacaaatg tatgatactc tccatgttct ccacatgggt ctgcaccagc
     3001 attttttatc ttctccacaa gttctctagt taattttttt cctaaaaatt cctctcctaa
     3061 gtgctttaag tttacttttg ttacaacact acaaaatcca ctgtctataa attcatatac
     3121 taaatcctct ctactctctt gccataatgg taaatctact ttcataccaa ctgcttcaca
     3181 tctatcagta tcccattttc tatgactttc tatatctata tctccatatg cacatatcgt
     3241 tgctcccatg tttttagctt ttattaaagc cctctcaaag ctctcttcat atccttctac
     3301 accacactca gcaactaata aaggtaatcc tatattgcta cttacttgct taagcatatt
     3361 atattctaaa ttatgtgtcc atgactttcc atgttcttca cttatagttg tcaagagtgc
     3421 cacaggctta tatccttttt tcaacattcg atttaatgct aatatactat ccttaccacc
     3481 actaaatgac attacaaatt tttcatcagc caattttaac acctctttca tctaactttg
     3541 ttttattaac ataattatac caaatttttg agttgcatag caaattatat taaattgtta
     3601 attcactata aagaatgtcc ctaatattaa aagtataacc cctatgtatt tgtataaatg
     3661 cattgtttct tgtcccaaaa ttacgctatc aataaaaaat ccaactaaaa cttgggatac
     3721 aagtgttaat ataagtgtat ttgatactcc taaatttgga attgccttta ctgtaaaata
     3781 tattattaaa cctccaaata ctcctcctaa taacaactta taattaaaac catttataga
     3841 tactagacac tttaagtcac ctgtaactat tatacttgcc aaaaaaaata ttgctcctac
     3901 agttaaactt ataaaggtgg ccactaatgc tgttgtactt tttcctagtt ctccatttat
     3961 aaaagcttca agagctgtag aaccacctgc taataatgca aatattattg cccataattt
     4021 tgtcatggac aaacctcctt ctatataatt ctattaatag aagtctatga tacaacctat
     4081 taattaatta ctaagtatag tttaattttt atacactagg ctttatttgg atatgaattt
     4141 ctgttataat tgttctatcg attttggagg tgattttatg tacacaaatt ttaatatcga
     4201 ttttaataac tttaataaaa aagaaaatgc tactaaattt atgttaatgg gagtattgct
     4261 cattatctta ggattattat gccttacatt taagacatta ggtataaaat taatttcttg
     4321 gacttttggt attgcacttt tattctttgc atatttaaat ttaaaaaata ttaatgaact
     4381 aaaaagatat gctactaaag aagaaattaa accctctatt aatatccaat gggttctaat
     4441 aattgcatgt atattacttt ttgtatttcc tcaaaaaata caaagcatat tttcattatt
     4501 tttagggttt tatttaatct ttaatcaact agtagcactt gtaaatagta agaataatcc
     4561 ttattctaaa tttacaactt ggaatatagt taagattcta tttggtatat gccttatatt
     4621 atctccactt tttttatcta gatttatagt atcgataatg tcttttttca taatattatt
     4681 tggactagtt ctatttttct ctggaaatac agcgagaaaa tattaattat tttctcgctg
     4741 tatttttata ttacttaaaa tagaatttgt ttaagttatc tatttgtggt aatatgtttt
     4801 ttgtacaagc tcagcgtaca attttattag aaataggtga tttttttgga taaatttaaa
     4861 tatgcttttg atgataaaag atatcatact tggaattact atcttagaaa tacttttgga
     4921 gaaaaagttt ttaaggtatc tataaatgca ggatttacct gcccaaacat tgatggaagt
     4981 cttggttatg gcggatgtac ctactgtagc aaagaaggct caggtgattt tgctggaaac
     5041 cctaaagata atcttatcag tcaattttat aatataaaag aaatgatgtt aaaaaaatgg
     5101 cctcatgcaa agtatattgg ctactttcaa gcctatacaa atacgtatgc tcctcttgaa
     5161 gttttaaaag aaaaatatga gactatatta gaacttgaag atgtcatagg tctttcaata
     5221 tccacaagac ctgactgttt gccagatgat gttgtagaat acttatctga actaaataaa
     5281 agaaccaatt tatgggttga acttggactt caaacaattc atgattctac atctaaaatt
     5341 ataaatagag gtcatgatta taaaacattt gttgaaggtg ttgaaaaatt aaagtctaaa
     5401 aatataaagg ttgtcgtaca cattataaat ggtcttcctg gtgaagatta taatatgatg
     5461 atggaaacag caaaggcagt tggtaaaatg ggtgtagacg gtataaaaat ccatctattg
     5521 catgttataa aagatactcc tatggaaaag atgttacaaa atggtatgtt aactcttatg
     5581 gaacaagatg aatatatcaa tcttgtctgt gaccaattag aaatacttcc tgaaactatg
     5641 ataatccata gactcactgg tgatggtaag agggatgaac tagtaggtcc tatatggagc
     5701 ctaaaaaaat gggaaatttt aaatcaaatt gatgatactt taaaggctag aaattcttat
     5761 cagggctgta agtttgttta atacaaaata ataactatag aaatggttat ataaatatag
     5821 tcctttctat agttatttag tatttacaaa acaaaatatt aatttgtaca tttatacaat
     5881 atataataaa aaatagatag tctaaactgt atctacttat attttatttg tactgtccac
     5941 tttattctat aaaatttata aatagttatt ttttaatacc aaatataatt tggtataatt
     6001 tttgttggat tctaaaatgt ataccaataa atccttttta agtatataat tctaaatact
     6061 ttcagttttt tcaatctgct ttaacttgtt ggatttataa aatattctta tttcatcata
     6121 agaaagatta agtgctacta ctgcagatat taacatcaac atcactccaa ataacttccc
     6181 taaggaaaaa ctttctccta atatagtgca accaattatt actgaaccaa ctaactctac
     6241 tgatgccaca acacctgcaa ctgacaactc tacaccttta gatatacctg ctgcataaaa
     6301 aatatatgca agtgcagctg gaacaatgcc taacattagc atacaagata aaacatctaa
     6361 atccttagca taatttaaga tttcccaagg tctcgaagaa ggaatcataa atatagctcc
     6421 aaataagaaa ctgtatacta atattgttga acttgaaaat tcctttaaag catttttact
     6481 aatagttggc ataagtgcat atgctattgc tgataatacc ccaagtaaaa gtccaaaagc
     6541 gtttaaacct tgaaaatcca aacgtccacc agtcactgcc ataattgcac caataaaaca
     6601 aagaataagt gaaaataatt tgcttctagt aatattttct ttataacata cttttgaaaa
     6661 tattgctaaa aacagtggtg atgtatatag taaaactgct gcaattgata cacctgcaat
     6721 gtcaattgct ttaaaataac ataaattaaa cattgcctga catataattc ctattattac
     6781 tgaatatata attccttttt tgcttatctt taagatttgt ggtgtcctta tagatgaata
     6841 tacgcctaaa actataaatc caaaaaacaa tcttgtaaat gctacttgtt ctgatgtcag
     6901 tccatttccc attaaaaact ttccaaataa tccaagtgta gcccataata accctgctat
     6961 tgctataaat acataccctt tacttttttc tcccatttta aattcccttc tatttttatt
     7021 tagtcaattt acactcttct ttttaagcaa aaattatgcc atcttaaacc aatatatatc
     7081 gactctaaaa ccctagtctt tccaataata ctaataagtt ttcaattttc ttatatttat
     7141 ttttcgctta ctttaagttg atttagactt attttaattg taaaatgagt tatatataac
     7201 ttatttaaag ttaaaataga ctgaaactta tataaatat
//
LOCUS       DAMJWP010000184         7201 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.191, whole genome shotgun sequence.
ACCESSION   DAMJWP010000184 DAMJWP010000000
VERSION     DAMJWP010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7201
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.191"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            497..1348
                     /locus_tag="PC700_002938"
     CDS             497..1348
                     /locus_tag="PC700_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HDF2582214.1"
                     /translation="MEINKKHFIQVFLTCLILVVICEFIGQRTIAIGKASIVLFPMLY
                     AVIIGLIITPDLLGKKIKALKKVVGEKEINIAGEVVGIALVMLGIKYGTTVGPNIDKI
                     IQAGPAFIAQEFGHILAPIVALPLALMFGMKREAVGATASISREPSLGVISEKYGINS
                     PEGSGVLGTYLMGTVLGTIYFSVLGSISIYSGLHPYALGMACGVGSGSMMTAAAGSLS
                     TMVPPEMADTILAYAATSNMMSSITGIIFLVFVSLPFTNFMYKILEPKFSRNKKTKEI
                     KSTEGEC"
     gene            1352..1822
                     /locus_tag="PC700_002939"
     CDS             1352..1822
                     /locus_tag="PC700_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582215.1"
                     /translation="MFANLNLKKSSYQLLVMSVIGVIILIGQRISVGTSIVTALPGMV
                     MLILAAMAAMIIKDLFPKSIFPAFGFATIIGLLLSMPYSPTSEVFLTNTNNINFMAIT
                     TPLLAFAGISVGNKIEALKEMSWKIVIISLIVFTTIFFACASIAHIVLSIQGKI"
     gene            1835..3145
                     /locus_tag="PC700_002940"
     CDS             1835..3145
                     /locus_tag="PC700_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2582216.1"
                     /translation="MNKEQLKSLVIKTIDENREKILKIGQGIYKNPEYGYKEFKTTEA
                     VSNFFEQELNLSVEKNIAVTGCKADANSSKKGPHISILGELDGISCKEHKDANEIGAS
                     HTCGHNIQIAGMLGAAVGLVKSGVLEHLDGKVSFMATPAEEFIELEYREQLKKEGKIT
                     YFGGKQELIKRGYFDDIDISMMFHASDLGEDKALVGPESNGFVGKKVKFIGKESHAGS
                     APHDGINALNAAMLAINNVNALRETFKESERVRFHPIITKGGDIVNVVPADVHMESYV
                     RARTINGMIDANERINKALMAGGMAVGADVEITEIPGYLPILRYKSLDNLFKNNLEDL
                     GLKGKVIDGGDFTGSFDFGDVSHIMPTLHPMIGGVKGALHTRDFEIVDEDLAYIIPAK
                     AMALTVVDLLFDNAKDAKDILDTFTPVMTKEEYLTFLEKHDKTL"
     gene            3357..4103
                     /locus_tag="PC700_002941"
     CDS             3357..4103
                     /locus_tag="PC700_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2582217.1"
                     /translation="MFNAGNRIINTWLYPIQDGFVLIDTGYENGFKHFKKRISKFNIK
                     IKDIRYIFLTHAHDDHVGFLNQILDESPNTKIVMHDKALEILYKGQNSFIGGCNSRTA
                     FLFCQIMKLFGKGEHSFSPLKQEFEKRCILVSDKNRKEIEKELNGKIIDTPGHTADSI
                     SLLIDDGVLFCGDSAMNGFPSTHKITIFAESKLEFIQSWKTIINTKPKMIYPGHGKPF
                     EYSKLEQNLIYVNKINLYPLHNSKDIHRFL"
     gene            4465..6006
                     /locus_tag="PC700_002942"
     CDS             4465..6006
                     /locus_tag="PC700_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HDF2582218.1"
                     /translation="MNDNVVKKKNFFDRTLNRIETVGNKLPDPVTIFLGLCVLLLILS
                     SLVGSMGISVVHPGTGETITAVNLLTVEQLQILLGNIVSNFQGFAPLGLVLVTMIGAG
                     VCDKTGLMTATIKASVSKIPETRVTLVVMTIGMLANIASDAGTILFPPLAALVYLGVG
                     RHPLIGLFSGYAAVCLGFAANIMISVNDILAASFTVPAAQMIDSGFQANATMNLFFMI
                     ASTFVLILLATWVTEKIVAPRFGKYEGNAELDVDGSLSDVEKKGLKKAGISILIYVAI
                     IVALSVIGKKPFLADPETGDLLSNNAPLMKGMVPIIALAFFIPGVVYGKTIGKIKKDK
                     DVVSIMASAMSDMGGYIILAFAASQFLQLFTNSNLGLILAVKGGEFLKSAGINGPLLL
                     IGFIILSCFINLFIGSASAKWAILAPIFVPMFMMVGYNPALTQMAYRIGDCITNPLSP
                     LFPYFPIILAFTRKYDKDAGMGTVIANMIPYSISFLIVWTILLILFMVFNIPLGPGIL
                     PSYSM"
     gene            6041..7147
                     /locus_tag="PC700_002943"
     CDS             6041..7147
                     /locus_tag="PC700_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF2582219.1"
                     /translation="MLDKNRLINNFMDMVRIDSPSNQELEMSKWLVNYLKERNIDAII
                     DDAGEKYGGNTGNVIAYIKGEEGSRPLCLCAHMDQVQPCLGVKPILDGNVVRSDGTTT
                     LGADDKAGIAAILEALEHVTTEKIPHRDIYLCFTICEEAGMHGVKNFNPDNLPCKDMV
                     ILDSGGAIGSIAYKAPAQQSIKISFHGKKAHAGIEPEKGLNAILVASHAISNMHIGRI
                     DPLTTSNIGKIEGGGATNIVTDKVTLTAEIRSHIPETLEYELNHMEKCCKDAASKFNT
                     TYTFEHSMSYPSFELSRDSHVFKLSEEAIRQVGVVPNPMVIGGGSDANILANLGYNCA
                     ILSLGMYDVHTVNEYVNIDELYDTAKIVYHMIKL"
BASE COUNT         2549 a          967 c         1212 g         2473 t
ORIGIN      
        1 aattcatttg cttattatca tttactttat tcaatttcta tatcaaaaat tagtatttgt
       61 acttaatact taaaattaat ttaaagtctt tacatattat ttattttcta ttgtaatatt
      121 catttccata ctttactaag ctacttccac tcatttattt taaaatttca atttactatt
      181 tctcaactct atattattat ttttcaattt tatattacta attttaaagc tttttagcta
      241 ttattcctac aatatttaaa caattttttc ataataaagc tatattacaa tatattcaaa
      301 atatgtaata ttttagatat ttttcgttga caatgttaaa tttttgtttt atccttaatt
      361 aaggacatgt tattgacatt tctgaaactt atttgaaata gttttttcat tttatttagg
      421 aaaacgtatt atacttaaga aaacgtattt tctcatttca gataagtaaa atatttattc
      481 ttttggggag gaaatcatgg aaattaataa aaaacatttt attcaagtat ttttaacttg
      541 cttaatcttg gtagtaatat gtgaatttat agggcaaaga actatagcta ttggaaaagc
      601 ctctatagta ctgttcccta tgctttatgc agttataatt ggacttataa taacaccaga
      661 tttattaggt aaaaaaatta aagcacttaa aaaagtggtt ggagaaaaag aaataaatat
      721 tgctggtgaa gttgttggaa ttgcactagt aatgcttggt ataaaatatg gtactacagt
      781 tggaccaaac attgacaaaa taattcaagc tggaccagca tttatcgctc aagaatttgg
      841 tcatatactt gcaccaatcg ttgcattacc tcttgcatta atgtttggaa tgaaaagaga
      901 agctgttgga gctactgcaa gtataagtcg tgaaccttct ttaggagtta tatcagaaaa
      961 atatgggata aactcaccag aaggtagtgg tgtacttggt acatatttaa tgggaacagt
     1021 tcttggtact atatacttta gtgtactagg ttcaatatct atttatagtg gtcttcaccc
     1081 atatgctttg ggaatggcct gtggtgttgg tagtggaagt atgatgacag cagctgctgg
     1141 ttcattatct accatggtac cacctgaaat ggcagataca atacttgcct atgctgcaac
     1201 aagcaatatg atgtctagta taacaggaat tatattctta gtttttgtat ccttaccatt
     1261 tactaatttt atgtataaga ttttagaacc taaattttcg agaaataaaa aaactaaaga
     1321 aattaaatct acagaaggag agtgctaata aatgtttgct aatttaaatt taaagaaaag
     1381 ttcatatcaa ttattggtta tgtctgttat tggagttata attcttatag gacaaagaat
     1441 tagcgttggt acatctatag ttacagctct tcctggtatg gtgatgctaa tattagctgc
     1501 tatggcagct atgattataa aagatttatt tcctaaatca atctttccag cttttggatt
     1561 tgcaactata atcggccttt tacttagtat gccatatagt ccaacatctg aagtattctt
     1621 aactaacact aataatatta actttatggc tataactact ccactactag cttttgcagg
     1681 aatttctgtt ggaaacaaaa tagaagcttt aaaagaaatg tcatggaaaa tagttataat
     1741 ttcacttatt gtatttacaa ctatattctt tgcatgtgcg agtatagctc atatagtttt
     1801 aagtattcaa ggtaaaatat aggaggaatt tattatgaat aaagaacaat taaaatctct
     1861 agtaataaaa actatagatg aaaatagaga aaaaatatta aaaataggtc aaggaatata
     1921 caaaaatcca gaatatggat ataaagaatt taaaactaca gaagctgttt caaacttttt
     1981 tgagcaagaa ttaaatttat ctgtagaaaa aaatatagca gttacaggat gcaaagcaga
     2041 tgcaaattct agcaagaaag gtcctcatat atctatactt ggagaattgg atggtatatc
     2101 atgtaaagaa cataaagatg ctaatgaaat aggtgcttct catacttgtg gacataatat
     2161 tcaaatagct ggaatgttag gtgctgctgt tggtttagta aaaagtggcg ttttagaaca
     2221 tttagatggt aaagtatctt ttatggctac tcctgctgaa gagtttattg agttggaata
     2281 cagagagcaa ttaaaaaaag aaggaaaaat aacttatttt ggtggaaaac aagaattaat
     2341 aaaaagaggt tattttgatg atatagacat atctatgatg tttcatgctt ctgatttagg
     2401 agaagataag gctctagttg gtcctgagag taatggattt gttggaaaaa aggttaaatt
     2461 tataggtaaa gaatctcatg ctggttctgc tccacatgat ggaataaatg ctttaaatgc
     2521 agcaatgctt gctataaaca acgtgaatgc acttagagaa acttttaaag agtctgaaag
     2581 agttagattc caccctatca tcacaaaagg tggagatata gtaaatgttg ttcctgctga
     2641 tgttcatatg gaatcatatg ttagagctag aactataaat ggtatgattg atgctaatga
     2701 aagaataaat aaggctttga tggctggtgg aatggcagtt ggagctgatg ttgagataac
     2761 tgaaattcct ggatatcttc caatattacg atacaaaagt cttgataatt tatttaaaaa
     2821 caatcttgaa gatttaggtc taaaaggaaa agttattgat ggtggagatt ttacaggctc
     2881 ttttgatttt ggagatgtgt ctcatataat gcctactctt caccctatga ttggtggtgt
     2941 aaaaggagct cttcatacaa gagattttga aatagttgat gaagatttag cttacataat
     3001 tcctgctaaa gcaatggcac ttacagttgt agaccttctt tttgataatg ctaaggatgc
     3061 taaagatata ttagatacct ttactccagt tatgactaaa gaagaatatc taacattcct
     3121 agaaaaacac gataaaactt tataagtcta tttttataat agggtggtat ctactgtaaa
     3181 agttatacca ctctttgttt ttgtaacagt atacttcttt tcttttagct tttagattaa
     3241 actaaaatat attatcctcc ttaaaacttt tagagatacg ttaaaatatc agtagaacta
     3301 tgctatattc tttctaatta atataaattt atattctaat tcaaggtggt gaaaaaatgt
     3361 ttaatgcagg aaatcgaatt ataaatacat ggttgtaccc tattcaagat ggctttgtct
     3421 tgattgatac tggatatgaa aatggtttta aacatttcaa aaaacgaatt tctaaattca
     3481 acataaaaat caaagatatt cgatacatat ttctaactca tgctcatgat gaccatgttg
     3541 gttttctaaa tcagattcta gacgaatctc ctaatacaaa gattgttatg catgataaag
     3601 ctttagaaat tctatataaa ggtcaaaact cttttattgg tggttgtaac agccgtactg
     3661 cctttctctt ttgccaaatt atgaaactat ttggaaaagg tgagcatagt ttttctccac
     3721 ttaaacaaga atttgaaaaa aggtgtattc ttgtgtctga caagaatcgt aaagaaattg
     3781 aaaaagaact aaatggaaaa attatagata caccaggaca tactgctgat tcaatttcac
     3841 tactaattga tgatggtgtt ttattttgtg gagattctgc aatgaatgga tttccaagta
     3901 ctcacaaaat taccatattt gcagaaagca aactagaatt tattcaatct tggaaaacaa
     3961 tcatcaatac aaaacctaaa atgatttatc ctggacatgg aaaaccattt gaatacagta
     4021 aattagaaca aaatctaatc tatgtaaata aaataaattt atatcctcta cataattcaa
     4081 aagatattca cagatttttg tagaaaatac aaagtaaatt tctttgatta attaaaaaat
     4141 ctaacacttt actacgctac ttattttaat aacttaatct tccactagct tttcataaaa
     4201 ttttgtttta aaattaactt taaagtgcat atgctattta aatacattag atataattcg
     4261 tcattaatct acttaaagca tatttaacta acaccactca accagaaaat tcagaaaact
     4321 gcattgacac aggaaaaagt ttattttata ctttttttag gacatattgt ggacacgtct
     4381 taaatcgaca agcagaattt atattgtaag atcaaccaca atacttattt tttaattata
     4441 tttttaaaca ttagggagga tattatgaat gataacgtag ttaagaagaa aaactttttt
     4501 gacaggacat taaacagaat agaaactgtt ggtaataaac taccagaccc agttacaata
     4561 tttttagggt tatgtgtact tttacttata ctatctagtc tagttggttc tatggggata
     4621 tcagtagttc atcctggtac tggagaaact ataactgcag taaatttatt gactgtagaa
     4681 cagcttcaaa ttcttttagg aaacatagtt agcaatttcc aagggtttgc tcctttgggt
     4741 ctagttctag taactatgat aggtgctggt gtatgtgaca aaacaggact tatgacagca
     4801 acaatcaaag catcagtaag caagattcct gaaacaagag taactcttgt tgtaatgact
     4861 ataggtatgc ttgccaatat agcttcagat gcaggtacaa tattatttcc accattagct
     4921 gcacttgtat atcttggtgt tggtagacat ccacttatag gtttattttc tggatatgca
     4981 gcagtctgtc taggatttgc cgcaaacatt atgataagtg tcaatgatat acttgctgca
     5041 agttttacag ttccagcagc tcaaatgatt gattctggat ttcaagccaa tgcaacaatg
     5101 aacttatttt ttatgatagc atcaactttt gttctaatac ttttggcaac atgggtaaca
     5161 gaaaaaattg tagctcctag atttggtaaa tatgaaggaa atgcagaact agatgttgat
     5221 ggaagtctta gtgatgtaga aaagaaaggg cttaaaaaag ctggtataag tatattaata
     5281 tatgtagcta ttatagtagc tcttagtgta atcggtaaaa aaccattctt agctgaccca
     5341 gaaacaggag atttactatc aaacaatgct cctcttatga aaggtatggt acctataata
     5401 gcactagcat ttttcatacc tggtgttgta tacggaaaaa ctataggtaa aataaagaag
     5461 gataaggatg tagtctctat aatggctagt gcaatgagtg atatgggtgg atatataata
     5521 ctagcatttg ctgcttcaca atttttgcaa ttattcacaa atagtaattt aggtctgata
     5581 ctagctgtaa agggcggaga atttttaaaa tcagcaggaa taaatggacc tttattgttg
     5641 ataggattta ttatattatc atgttttata aatttattta taggtagtgc atctgcaaag
     5701 tgggctattc ttgcacctat atttgtacca atgtttatga tggttggata caatccagca
     5761 cttactcaga tggcttatcg tataggtgat tgtataacaa atccacttag cccattattt
     5821 ccgtacttcc ctattatact agcattcaca agaaaatacg ataaagatgc tggtatgggt
     5881 actgtaattg ctaatatgat accatactct atatcattct taatagtgtg gactatacta
     5941 ttaatattgt ttatggtatt taatattcca ctaggacctg gaatacttcc ttcatatagc
     6001 atgtaattta tttaaatctt tctaaaagga gaaataattt atgttagata aaaatagact
     6061 tataaataat tttatggata tggttagaat tgatagccca tctaatcaag aattagaaat
     6121 gtctaagtgg ttggtaaact acttaaaaga aagaaatata gatgccatta tagatgatgc
     6181 aggtgaaaaa tatggtggta atacaggtaa tgtcattgcc tatataaaag gtgaagaagg
     6241 aagtagacca ctttgtttat gtgcacacat ggaccaagtt caaccttgtt taggtgtaaa
     6301 accaatacta gatggtaatg tagttagaag tgatggaact actacacttg gtgcagatga
     6361 taaagctgga atagctgcta tacttgaagc tttagaacat gttactacag aaaaaatacc
     6421 acatagagat atttatcttt gttttacaat ctgtgaggaa gctggtatgc atggtgtcaa
     6481 aaactttaat cctgacaact tgccttgtaa agatatggtg attttagatt ctggtggagc
     6541 tattggttcg atagcataca aagctcctgc tcaacaatct ataaaaatct cttttcatgg
     6601 aaagaaagca catgctggta tagaacctga aaaagggtta aatgctatat tagtagcctc
     6661 tcatgcaata agcaatatgc acataggcag aatagatcca ttgactactt caaatatagg
     6721 taaaatagaa ggtggtggag ctacaaacat agtaactgat aaggttacat tgactgctga
     6781 aattaggtct catatacctg aaactttaga gtatgagctt aatcatatgg aaaaatgttg
     6841 taaggatgct gcatctaaat ttaatactac ttataccttt gaacatagta tgtcttaccc
     6901 tagttttgaa cttagtagag atagtcacgt tttcaagtta agtgaagaag caataagaca
     6961 agttggtgtt gttccaaatc caatggtaat tggtggtgga agtgacgcaa atatattagc
     7021 taatcttgga tataattgtg ctatattaag tctaggtatg tatgatgtac acacagttaa
     7081 tgaatatgta aatatagacg aattatatga tactgccaaa atagtttatc atatgattaa
     7141 attataaata tcataatata cctctttaaa ttataaaaat aaaaacctat gtatcttcaa
     7201 c
//
LOCUS       DAMJWP010000185         7158 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.192, whole genome shotgun sequence.
ACCESSION   DAMJWP010000185 DAMJWP010000000
VERSION     DAMJWP010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7158
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.192"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(123..962)
                     /locus_tag="PC700_002944"
     CDS             complement(123..962)
                     /locus_tag="PC700_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF2582220.1"
                     /translation="MSDIKNEVSKKTLSKKDVIKSWLRWFFFAQSNYNYERLQSTAFS
                     HSMLPVLKKLYPDKEELKQEVETHLAFFNTEPICGCVIHGITIAMEEEKANGADEISG
                     DGMNAIKTGLMGPLAGIGDTLTQGVITPIVLAVCIGLTEGGASIAGPILFVIAQYIIM
                     TSISFGMWTNGYKYGKKAVESILQGGIVNKVIEGASILGTLVMGGLVGRFINLSTPIS
                     YTSGDFKFSLQTDLLDKIFPGLIPLVLTLLVLFALKKGLSPIKIMFILIVVGAITGIL
                     GLF"
     gene            complement(986..1744)
                     /locus_tag="PC700_002945"
     CDS             complement(986..1744)
                     /locus_tag="PC700_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2582221.1"
                     /translation="MHISLIQAVLIAIFYYLSWSPWLTYVGFFTWNRPLLAGFVTGII
                     LGDPVQGAIIGAGINMIYLGFISAGGAQMGDPAFAGYVGTALAIASKLDVSTAMAIAV
                     PLGTVATVLWIGKMTVNSFFAHWADREVQKGNVDRVAFINIVPPQILLFAMSFIPALL
                     VVYFGPNAIDGMLEVMNENVLHVFNVIGAMLPALGIAMNLKLIGNKFTMPFFILGILM
                     AVYFKVDIIVISVIGVILALTITSIKYGKDSATS"
     gene            complement(1791..2264)
                     /locus_tag="PC700_002946"
     CDS             complement(1791..2264)
                     /locus_tag="PC700_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2582222.1"
                     /translation="MSNIALTRIDDRLIHGQVITAWCKITSAKRIVIVDDLVVKDPFI
                     VQVLQMAAPSTVKVEVHDVESGAEVLKSHNGDEHLIVLVKYPKTVLGLVNSGVDLKEL
                     NVGGMGAGLGRKSFYKNISVSDEEKEIFRELISKDVKCFIQIVPDAKKIDVGTLL"
     gene            complement(2293..2709)
                     /locus_tag="PC700_002947"
     CDS             complement(2293..2709)
                     /locus_tag="PC700_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2582223.1"
                     /translation="MIHMTGIIIATHGNMAKAMLESAELIVGKQENVETLGLNHGDSI
                     DEFSRRLEDRIEKYKDEGVLVLLDFYGGTPFNTSAIIINKFAKVCELECLTGVNLPML
                     LELFLNRDTMKLKDLKNLCEEVGISGIKDVKTVLKL"
     gene            complement(2756..3808)
                     /locus_tag="PC700_002948"
     CDS             complement(2756..3808)
                     /locus_tag="PC700_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF2582224.1"
                     /translation="MKSVRFYGIRDTRVEDVDVPKILEKDDVIIKVKVAGICGSDISK
                     YSKTGPHMVGEILGHEFSGEVAQVGKEVRSFKIGDRVAVCPAMPCFECDECKKGLYSR
                     CNNVAIIGNKELGGCFAEYTKVKERNLIKIPDEISYETAAALEPVCIAGHGLFRSEAK
                     VGDTVVVLGTGTIGLFSIQWAKIFGSTKIIAVDVFDEKLDLAKELGADICINAKEKNI
                     VEEIKRLTDGDGADIVIESAGTPMTCGQVLLLAKKGGTVLYAGVPYGDVALTREQFEK
                     IVRSELTVKGTWFGNSFPFPGKEWSAGLYHMQKGDMNVEKLVTHRINLEEVPAYFEKV
                     YKRDIFFGKIMINIDN"
     gene            4204..4884
                     /locus_tag="PC700_002949"
     CDS             4204..4884
                     /locus_tag="PC700_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDF2582225.1"
                     /translation="MKKVYQGKTKDVFELDNGNYLLKFKDDVTGKDGVFDPGENSVGL
                     SIDGIGRANLETSVKFFEILNNAGIKTHYVSANLEEATMEVLPAKVFGNGLEVICRYR
                     AVGSFLRRYGSCVEEGAKLDCYVEATLKDDDRCDPLITSEGLEALNIMTQAQFDSMKS
                     MTQKISNIVRDTIAEKGLELYDIKFEFGFYNDEVILIDEIASGNMRVYKDGVIVDPMD
                     LTALLLDK"
     gene            complement(5017..5337)
                     /gene="sugE"
                     /locus_tag="PC700_002950"
     CDS             complement(5017..5337)
                     /gene="sugE"
                     /locus_tag="PC700_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDF2582226.1"
                     /translation="MKWLFLFVAGLFEVFWAFQLKNSHGFSKLTPSILTVVGMIVSFY
                     FLSLALKHLPLGTSYAIWTGIGTVGTAIVGMAILNEPISISRIACIGFIVVGIVGLKL
                     LSTN"
     gene            5719..6546
                     /locus_tag="PC700_002951"
     CDS             5719..6546
                     /locus_tag="PC700_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HDF2582227.1"
                     /translation="MNFYKIAVCQMMTTENKIENINHAVDMVTEAAINGAKIIVLPEM
                     FNCPYENKYFPKFAEEYPGETTTILSKLAEKHGIYLVSGSIPELEYGKIYNTCYVFDK
                     NGALIGKHRKMHLFDIEVTGKVSFKESDTLTAGNDVTVIDTEYGKVGIAICYDIRFPE
                     LSRLMALKGVEIVILPAAFNMTTGPAHWELSIRMRALDNQIFYVGAAPARNMNASYIA
                     FGNSRISDPWGRIIAQADEKECIIYADIDRDLIPDIRQQLPLLKHRRTDLYELNTLK"
BASE COUNT         2420 a         1302 c          836 g         2600 t
ORIGIN      
        1 aaaatttaat ctattataaa attaccctaa ttgctaaact ttttaatata aattttccaa
       61 catcttacaa atataatcaa tagatataga atcctaattt acacaccaac atatatattt
      121 atttaaaata gtcccaaaat acctgtaata gctcccacta ctatcagtat aaacattatt
      181 tttattggag aaaggccttt ttttaaagca aagagtacta ataaagtaag taccaaagga
      241 attaatcctg gaaatatctt atcaagtaaa tccgtttgaa gactaaattt aaaatcacca
      301 ctagtataac ttattggtgt agataaatta ataaacctac ctacaagtcc ccccatgact
      361 aaagtaccta atatagaagc cccttctata accttattta ctattccacc ttgaagtata
      421 ctttcaacag cttttttccc atacttatat ccatttgtcc acattccaaa agaaattgaa
      481 gtcattatta tatattgagc tattacaaat aatataggac ctgcaattga tgctcctccc
      541 tcagttaaac caatacaaac agctaacact ataggagtta taactccttg agttaaagta
      601 tcaccaattc ctgctagtgg acccatcaag cctgttttga tagcattcat accatctcca
      661 cttatttcat ctgctccatt agctttttct tcttccatag ctattgttat tccatgtata
      721 acacatccac atataggttc tgtgttaaaa aatgctaaat gagtctccac ttcttgtttt
      781 aactcttcct tatctggata taatttttta agtactggca acattgaatg tgagaaagct
      841 gttgattgca atctttcata attgtaatta gattgcgcaa agaaaaacca tcttaaccat
      901 gattttatta catctttttt gcttaacgtt ttcttagata cttcattttt tatatctgac
      961 ataatatttt ccccctcaca tgtatttaag atgtagcact atcttttccg tacttaattg
     1021 aagtaattgt aagagcaagt attactccaa taacagatat tactattata tctactttaa
     1081 aataaacagc cattagtatt cctaatatga aaaagggcat agtaaacttg tttccaatca
     1141 attttaaatt catagcaatc ccaagtgctg gcaacatagc cccaattaca ttaaatacat
     1201 gtaacacatt ctcattcatt acttccaaca taccatctat cgcatttgga ccaaaataaa
     1261 caactaataa tgctggtata aaactcattg caaataacag tatctgtggt ggtactatat
     1321 ttataaatgc tactctatca acattccctt tttgaacctc tctatcagcc caatgtgcaa
     1381 aaaaagaatt tactgtcatt tttccaatcc aaagtacagt tgcaacagtt cccaatggta
     1441 cagctattgc cattgcagta cttacatcta gcttagatgc tatagctaaa gcagtaccta
     1501 catatcctgc aaaagctggg tcccccatct gagctccccc tgctgatata aaacctagat
     1561 atatcatatt tatacctgct cctataattg ctccttgaac agggtctcct aatataattc
     1621 ccgttacaaa tccagccaat agaggtctat tccatgtaaa aaatccaaca taggtcaacc
     1681 acggactcca agaaagataa taaaaaattg ctattaatac tgcttgaatt agtgaaatgt
     1741 gcatattctt cctccctatt ttcttttaaa gatttacaag atagtctttt ttataataat
     1801 gttcctacat caattttctt tgcatctggt actatttgta taaagcattt aacatctttt
     1861 gatattagtt ctctaaatat ttctttttct tcatctgaca cagatatatt tttataaaaa
     1921 ctttttctac caagccctgc tcccatacct ccaacattca attcctttaa atctacccca
     1981 ctatttacta aacctaagac agttttaggg tatttgacca atactattaa atgttcatct
     2041 ccattatgtg attttaaaac ttcagcacct gattctacat catgtacttc aaccttaaca
     2101 gttgatggag ctgccatttg taaaacttga actataaatg ggtcctttac tactaaatca
     2161 tccacgatta ctatcctttt tgccgatgta atcttacacc aagcagtaat aacttgccca
     2221 tgtattaatc tatcatcaat tcttgttaat gctatattac tcataattat tcctccagat
     2281 ttttaattta ttttacaact ttaaaacagt tttaacatct ttgattcctg atatcccaac
     2341 ttcctcacat aaattcttca agtcttttaa cttcatagta tctctgttta aaaatagctc
     2401 taaaagcata ggtaaattta cacctgtcaa acattctaac tcacatacct tagcaaattt
     2461 atttattata atagcagatg tattaaatgg agtcccgcca taaaaatcaa gcaatactaa
     2521 aaccccttca tccttatatt tctcaattct atcttctaac cttctactaa actcatctat
     2581 gctgtcacca tggtttaatc ctaaagtttc tacattttcc tgtttgccta ctattaattc
     2641 agcactttct aacattgctt tagccatatt tccatgtgtg gctataatta tccctgtcat
     2701 atgaatcata cctttcttaa ccttgtttat atggattatt atttagtact ctaatctaat
     2761 tatctatatt aatcattatc ttaccaaaga aaatatccct tttgtatact ttttcaaaat
     2821 aagctggtac ttcttctaaa tttattctat gtgttactaa tttttcaaca ttcatatcac
     2881 ccttttgcat atgatacaat ccagcactcc attcttttcc tgggaatgga aaagagtttc
     2941 caaaccaagt tcccttgaca gttaattcac ttcttactat cttttcaaat tgttctctag
     3001 tcaaagctac atccccatat ggtacacctg catacaatac tgtccctccc tttttagcta
     3061 acaacaatac ttgcccacat gtcattggag tcccagccga ttcaataaca atatctgcac
     3121 catctccatc agttaacctt tttatttctt ctacaatatt cttttctttt gcatttatac
     3181 aaatatctgc ccctaactct ttcgccaagt ctaacttctc atcaaataca tctacagcta
     3241 ttatttttgt agagccaaat atctttgccc attgtattga aaataatcct attgtacctg
     3301 taccaagtac tacaacagta tctccaactt tagcttctga tctaaaaaga ccatgccctg
     3361 ctatacatac tggttctagc gcagcggcag tctcataact tatctcatct ggtattttta
     3421 ttaaattcct ctctttcacc tttgtatact ctgcaaaaca acctccaagt tctttatttc
     3481 ctattattgc tacattatta catcttgaat acaatccttt tttacattca tcacattcaa
     3541 aacatggcat agctggacaa acagcaactc tatctcctat tttaaaactc cttacttctt
     3601 taccaacctg tgccacttct ccagaaaatt catgccctaa aatttctcca accatatgtg
     3661 gacctgtctt tgaatatttt gatatatcag aaccacatat tccagctact tttactttaa
     3721 ttattacatc atctttttct aatatttttg gtacatccac atcctccact cttgtgtctc
     3781 ttataccata gaatctaact gacttcacta ttaaccaccc ctacttttta tttgagaata
     3841 tatccctatt gtttatatgt taaatatcta aagcaacttt catgccaata tatgtaagca
     3901 ttgcattcat gcattttata aaattatcat taaatgtgtg tcaaagtgtg tcaaaagttt
     3961 cttatattaa aacttatgtg tgtcaatata aacgacacta acattttata ttagttctct
     4021 tattataagt tttataaaat gtactttttt tgttattttt ataaaattgt tcggaaattt
     4081 cccttctatt tgtgatataa tattaaatat aaggtatcaa tataaatttt catgcgaata
     4141 tcagaacttt tttgacacta atgccttatt caatataata atttaacttt ttggaggttg
     4201 tttatgaaaa aagtgtatca aggaaagact aaagatgttt ttgaattaga taatggaaat
     4261 tacttactta aatttaaaga cgatgtaact ggtaaagatg gagtatttga cccaggcgaa
     4321 aatagtgtag gtctaagtat agatggaatt ggtagagcta atttagagac atctgttaag
     4381 ttctttgaaa ttctaaacaa tgctggaatt aaaactcatt atgtaagtgc taatttagaa
     4441 gaagcaacta tggaagttct accagcaaaa gtttttggta atggattaga agttatttgt
     4501 cgttatagag ctgttggtag tttcttacgt cgttatggtt catgtgttga agaaggtgct
     4561 aaactagatt gctatgtaga agcaacttta aaagatgatg accgttgtga ccctcttata
     4621 acatctgaag gtttagaagc tttaaacatt atgactcaag ctcaatttga ttcaatgaaa
     4681 agcatgactc aaaaaatttc taatatagtt cgtgatacta tagctgaaaa aggtcttgaa
     4741 ctttatgata tcaaatttga atttggattc tacaatgatg aagtaatctt aatagatgaa
     4801 atagcatctg gaaatatgcg tgtttataaa gatggtgtaa tagttgaccc tatggattta
     4861 actgctcttt tattagataa atagtatata catataattt tcaaatataa taaacttata
     4921 aataagataa gctatgaagt aaagactttt taaatgttga ttatttttaa acattttctc
     4981 gcctttaatt tcatagctta tttaaattaa tatactctaa ttagtagata atagctttaa
     5041 ccctacaatt cccaccacaa taaagcctat acatgcaatt cttgatatac taataggctc
     5101 atttaagata gccatgccta caatagctgt accgacggta ccaattccag tccatattgc
     5161 atatgaagtt ccaagtggaa gatgttttag tgctaatgac aaaaaataaa aacttacaat
     5221 cattccaaca acagtaagta tacttggagt taatttagaa aatccatgtg aattttttag
     5281 ttgaaaagcc caaaacactt caaacaaacc tgcaacaaac aaaaataacc atttcataaa
     5341 ctcatccctc ttttcaaatg tatctcttat ttttccaata aaataacctg agaaatatta
     5401 taaaatatct cccaggtttt tgtccttccg tgttcacaac aaaaactatg ttgtgggttt
     5461 tatctcggac caggccaatc attaataatt gcaaaaccct ataaaactct attaattatt
     5521 ctattttatc tataataata ctatcaacat atataaaaaa agtcaatata tgggttccat
     5581 ctataataaa aataactttt atataccttt taatattctt attcacttct ctccataccc
     5641 atttaggtca atctaagaat atctaatttt tatttaatat gttataataa cataaaaata
     5701 ttgaaaggag taatttctat gaatttctat aaaattgcag tatgtcagat gatgaccaca
     5761 gaaaataaaa ttgaaaatat caatcatgct gtagatatgg taacagaagc tgccatcaat
     5821 ggagctaaaa ttatagtttt gccagaaatg tttaactgcc catatgaaaa taaatatttt
     5881 cctaaatttg ctgaagaata tccaggtgaa acaacaacga tattgagtaa attagctgag
     5941 aaacatggaa tttatctagt ttctggtagt attcctgagt tagaatatgg aaaaatctat
     6001 aatacttgct atgtatttga taaaaatgga gctctaattg gaaaacatcg taaaatgcac
     6061 ttatttgaca ttgaagttac aggaaaagtt agttttaaag aatcagatac tcttacagct
     6121 ggaaatgatg tcactgtaat agataccgaa tatggaaaag ttggtatagc tatttgctat
     6181 gatattcgtt tccctgagtt atcacgttta atggctctta aaggtgttga aatcgttatc
     6241 ttacctgctg catttaatat gactactggt cccgcccatt gggagttatc tataagaatg
     6301 cgtgctttag ataatcagat attctatgtt ggtgctgcac ctgctagaaa tatgaatgct
     6361 tcttatatag cttttggaaa ttcaagaatt tcagaccctt ggggaagaat aattgctcaa
     6421 gctgatgaaa aagaatgtat tatttatgct gacattgata gagatttaat tcctgacata
     6481 agacaacagt taccacttct taagcacaga agaactgatt tatatgaact taatactttg
     6541 aaataaaaca gaacataacc ccataaatca taaattaagt gttaaaaaaa tttatggggt
     6601 tctcattaat ttgatttaaa attaatttaa taaaaataat gctaaaatta atttaattaa
     6661 ataatactaa taattactaa tgattatgct aattgtcagc atttactaat ttaatcacta
     6721 ttagttattt cttttattaa atatctttac tttttttact tattatgtct tctagcttta
     6781 catgtggatt atggtatttc tttctatttt tagtcacatc tttctattga attgcttgct
     6841 taaaacacca atgcacacat gccagataat gttcacaatt atgtaaaaat attgctctac
     6901 cttcttcaat gttgatatta tctctcctag ttaatattat ctttcctaaa atttctttta
     6961 attaaaaaac aacattagtt gatatatcaa gtatatttta agcaactgaa aaaataataa
     7021 taattgatat atcaactaat taatgtagat taattcattt ttataattta tatacttttt
     7081 aattaatcta cctttttaat taaaatcctt cctttctatt tatccatttt caacttaaat
     7141 tataagattt catcagct
//
LOCUS       DAMJWP010000186         7068 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.193, whole genome shotgun sequence.
ACCESSION   DAMJWP010000186 DAMJWP010000000
VERSION     DAMJWP010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7068
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.193"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            91..471
                     /locus_tag="PC700_002952"
     CDS             91..471
                     /locus_tag="PC700_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2582228.1"
                     /translation="MINKIGKITLYVNNQEDAKLFWTEKLNFVVTYEKEMGPNMKWVE
                     VAPSKNEFTTFVLYDKNLMMKQNKEINVSHPSIILSTRDINGTYEQMANNEVEVGELM
                     EMPYGRMFSFKDQDGNDYLVREDK"
     gene            596..796
                     /locus_tag="PC700_002953"
     CDS             596..796
                     /locus_tag="PC700_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582229.1"
                     /translation="MFKKMAVLKDIATKIGRKKAYELLEMVEGNDAFVAEVKIKKNGI
                     ESKKEEIMLKDNQKIILEYIEG"
     gene            992..1720
                     /locus_tag="PC700_002954"
     CDS             992..1720
                     /locus_tag="PC700_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2582230.1"
                     /translation="MLEIKNLSFNVESNNEELGIINDVSLSFERGKLIVITGPNGGGK
                     STIAKLIMGIEKATSGQIILDGEDITNLSITERAKKGIGYAFQQPPRIKGMTVENLLT
                     LAHGKPLSTDVCCQYLTDVGLCSKDYLNREVDNSLSGGEMKRIEIATLFARDLKVSIF
                     DEPEAGIDLWSFGKLNESFKKIHEESNQTIIIISHQERILELADEIIVLQDGSVKSHG
                     TKEAILPEIMCQVNSSCELMKDMN"
     gene            1742..2659
                     /locus_tag="PC700_002955"
     CDS             1742..2659
                     /locus_tag="PC700_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HDF2582231.1"
                     /translation="MNKIDLNLLEKVAELRGTPLGAYNIRKNGQGVERHSTENVTIIP
                     KTDKPGIDIIVKPNTKGENVHIPVIVTEAGVQDMVYNDFEIGENADVVIIAGCGIHNC
                     GCDDSEHNGIHRFFLRKGARLKYVEKHYGEGDGEGKRILNPVTIVNMDEDSYCEMETI
                     QIKGVDSTKREMDANLGKGAKIVVFEKLLTHGNQVAESNMKVILEGENSSAQVVSRTV
                     GQDNSKQKFNPCVIGDSLCSAHVQCDSIIMGNSQISAIPEIAANHPDALLVHEAAIGK
                     IAGDQILKMMTLGLTEEEAEQQILNCFLK"
     gene            2801..3985
                     /locus_tag="PC700_002956"
     CDS             2801..3985
                     /locus_tag="PC700_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF2582232.1"
                     /translation="MKNLLIRNGTIISPTDGIKFEKRDILVLHGKIYAIGLNLKEKIR
                     KEKLLTNKEFEIINADNMYISPGFIDVHTHCYKRKLPTGMDSDSIGIEKGSTVIFDAG
                     TAGPSNYYDFKKKYMDECKTEVYSFLNVTNEGLNILNESTSLDVINFDKVESIVEKNI
                     EKIKGIKVKASKFQSNESGIDIIKKSKQVAKKLNLPLVVYIGEYPSYIDEVMNILGKG
                     DVVTPVYGNSTNGILKESGTLRKSVINAKNRGVLFDVCHGVDQFDFKVFKKAIKQGLE
                     PDLISTDMHIKNMKDVNYSLLNVINKIMELGISLEKCIEKVTEYPTKIFDLHGNKGRI
                     KIGGEGDFTIFTMEECNDIIKDYNNNELVLNKKLRLQYTVKSWMKSSEVYRHGYENTI
                     IN"
     gene            4092..4280
                     /locus_tag="PC700_002957"
     CDS             4092..4280
                     /locus_tag="PC700_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDF2582233.1"
                     /translation="MKAFVDKDVCVACGACIGTCPDVFSMSDEGHSVAIEGEIPADLQ
                     DLCKEAEEGCPVSAITVK"
     gene            4430..5080
                     /locus_tag="PC700_002958"
     CDS             4430..5080
                     /locus_tag="PC700_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDF2582234.1"
                     /translation="MKKNYEIVLFDLDGTLTDPKEGITKSIQYSLNSFGIKEDLENLD
                     QFIGPPLHDTFKEYYKFEDKKAKEAVEKYREYFADKGIFENKIYENMKEILEMLYKNG
                     KILLVATSKPTVFAETILRYFDIDRYFKYIAGSNLDGTRVNKNEVIQYVLDLCNVKDK
                     DKVIMVGDRKYDIIGAKKIGIDSIGVLYGYGSFEELSESEPTYIVENVESIKDILL"
     gene            5183..6121
                     /locus_tag="PC700_002959"
     CDS             5183..6121
                     /locus_tag="PC700_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDF2582235.1"
                     /translation="MKSILIMGGSDFIGSALAKRLIKCGYQIDILTNGKKEIDYNGFK
                     EHLICDRKVRKDMENIITGRKYDYIYDMTAYTKEDVSNLIDFISMDNLKKYIVLSAGA
                     VYKDSGRNIKEENEKGENENWGKYGLNKKEAEDFIINSPIPYIIIRPTYIYGENNNLY
                     REYYFFEKIEKNEKIPVPKGKQVSNQFIYIGDLVKVLESIMKNPHVREAYNVTNPQLI
                     SWDDLIYTCGEIIGKEPIIKYVDMEKVEFRERTYFPFRNIDFNLDINKLIEHGLYIPN
                     VLLKEGLTATYKWFSANKPKMHDRKMNKVESVMQIV"
     gene            6147..6998
                     /locus_tag="PC700_002960"
     CDS             6147..6998
                     /locus_tag="PC700_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2582236.1"
                     /translation="MNSKIKYFLKFFILIFILIFIYSVAIEPSLLIVKKYNISENGAL
                     IKNDTIESKSESVKVVQISDTQIGSFYSTKNLKKVANKINTLNPDIIVFTGDLIDYSN
                     KNPSVDEITTILSSMNARLGKFSVFGNHDYMYKLPRYYRQIMKNSNFNLLVNENKKIK
                     LKDDKYINILGVDEILNGNPNIKYLESQIDNKNFNLLLAHEPDLVDMLSKDTMNLVLS
                     GHSHGGQIRLPIKGALVTPPYGRKYTKGFYDINGNHLYVSSGLGSTKLPFRFFNIPEI
                     IEFNIII"
BASE COUNT         2918 a          666 c         1272 g         2212 t
ORIGIN      
        1 atatatatct atagaaaata atatatatgg gtatattatt aaatataaaa aatattaggt
       61 atttatttaa taataaaggt gggtattgtt atgataaata aaataggaaa aataactctc
      121 tatgtaaata atcaagagga tgcaaagttg ttttggacag aaaaattgaa ctttgtagta
      181 acttatgaaa aggaaatggg tccaaatatg aaatgggtgg aagtagcacc aagtaaaaat
      241 gaatttacaa catttgtgtt gtatgataaa aatctaatga tgaagcaaaa caaagagata
      301 aatgtatctc atccatcaat aattttaagt acaagagata ttaatggaac ttatgaacaa
      361 atggcaaata acgaggttga agttggggaa ttaatggaga tgccatatgg aaggatgttt
      421 tcttttaaag accaagatgg aaatgattat ttagtcagag aggataaata attttacaaa
      481 gtattaaatt tactcaatat ctgatataat tagagacaat aattattata aactagtcta
      541 ggattagaat tatataatta aaaattaagt gtaaattttg agtagaggtg ataaaatgtt
      601 taagaaaatg gcagtactaa aagatatagc aactaaaata ggtcgtaaaa aagcgtatga
      661 actattagaa atggttgaag gtaatgatgc ctttgtagct gaggtaaaga taaaaaagaa
      721 tggaatagaa tctaaaaaag aagaaattat gttaaaagat aatcaaaaaa taatattaga
      781 gtatatagaa ggttaaattt taaatacata taagttgata tactaaattt ctgagatgag
      841 tttacataaa actcatcttt tttacttttc aataactttg ttttattatt taatatacat
      901 tttatctgct tagagttgct gatatataaa aattactgat ataataaaag aatggatatt
      961 aatgtaatat tttatagaaa agaggggtat tatgcttgaa ataaaaaatc tttcctttaa
     1021 tgttgagtct aacaatgagg aactaggaat aataaatgat gttagccttt catttgagag
     1081 gggaaaactt attgttatca caggtccaaa tggtggagga aaatctacaa ttgctaagct
     1141 tattatggga attgaaaaag ctacgagtgg tcagataatt ttagatggag aagatataac
     1201 aaatcttagt attacagaaa gagcaaaaaa aggaatagga tacgcatttc aacaaccacc
     1261 aagaataaaa ggtatgacag ttgaaaattt acttacactt gctcatggaa aaccattgtc
     1321 tactgatgta tgttgccaat accttacaga tgttggattg tgttcaaaag actatttaaa
     1381 tcgtgaagtt gataactctc tttctggagg ggaaatgaaa agaattgaga tagcaactct
     1441 ttttgctaga gatttaaagg tttctatatt tgatgaacct gaagcaggga tagacctttg
     1501 gagctttgga aaactcaatg aaagttttaa gaaaatacat gaagagtcta atcagactat
     1561 aatcataatt tctcaccaag aaagaatatt agagttagct gatgaaataa ttgttctaca
     1621 agatggttca gtaaaaagcc atggaacaaa agaggctata ttacctgaaa tcatgtgtca
     1681 agttaattca agttgtgaat taatgaaaga catgaattaa ttagaaagga aggcaaatta
     1741 aatgaacaaa atagatttaa atcttttaga aaaagttgct gaacttcgtg gaactccatt
     1801 aggagcatac aatatacgta aaaatgggca aggagtagag agacattcaa ctgaaaatgt
     1861 aactataatt cctaaaactg ataaacctgg aatagatatt atagtaaagc caaatacaaa
     1921 aggagaaaat gtacatatac cagtaatagt aacagaggct ggtgtacagg atatggtata
     1981 taatgatttt gaaattggag aaaatgcaga tgttgttata attgctgggt gtggtataca
     2041 taactgtggt tgtgatgata gtgaacataa tggtatacat agatttttct tacgtaaagg
     2101 agctcgccta aaatatgtag aaaaacatta tggtgaaggt gatggagaag gaaagagaat
     2161 tttaaatcca gttactattg tcaatatgga tgaggattca tattgtgaaa tggaaacaat
     2221 tcaaattaag ggtgtagatt caactaaaag agaaatggat gcaaacctag gtaaaggagc
     2281 taaaatagtt gtatttgaga aattattaac tcatggtaat caagttgcag aatctaatat
     2341 gaaggttatc ttagagggtg aaaactcttc tgctcaagta gtttctcgta cagttgggca
     2401 agataattct aagcaaaagt ttaatccttg tgttattggg gattcccttt gttctgcaca
     2461 tgttcaatgt gattcaataa ttatgggaaa ttctcaaatt agtgctatac ctgaaattgc
     2521 tgcaaatcat ccagatgctc ttttagtaca tgaagcagct attggaaaga ttgcaggaga
     2581 ccaaatacta aagatgatga cattaggtct tactgaagaa gaagccgagc aacaaatact
     2641 taattgcttc ttaaagtaaa ttaaagattt tatttaattt aatataatta atatagtata
     2701 atatagtgaa taagtctaaa atatttttat ttttaggctt attttcttta tgtaagaaaa
     2761 gctattagag aaattatatt gtaattagga tgtggaataa atgaaaaatt tacttataag
     2821 aaatgggaca ataatatctc caacagatgg tatcaaattt gaaaaaagag atatcttggt
     2881 tttacatggg aaaatatatg ctattggatt gaaccttaaa gagaaaataa gaaaagaaaa
     2941 attacttaca aataaagagt tcgagataat aaatgccgat aatatgtata tttccccagg
     3001 atttatagat gttcacaccc attgttataa gagaaaatta cctacaggta tggattctga
     3061 ttcaattgga attgaaaaag gttctacagt tatatttgat gcaggtacag ctggtccctc
     3121 aaactactat gattttaaaa aaaagtatat ggatgagtgt aaaacagaag tatattcttt
     3181 tttaaatgtt acaaatgagg gactaaatat attgaatgaa tcaacttctt tagatgttat
     3241 aaattttgac aaggttgagt caattgtaga aaaaaatata gaaaagataa agggtataaa
     3301 agtaaaagcg agtaaatttc aatctaatga aagtggtata gatataatta agaaatcaaa
     3361 gcaagttgct aaaaaattaa atttaccctt agtggtatat ataggagagt atccatcata
     3421 tatagatgaa gtaatgaata ttttgggaaa aggagatgtg gttacaccag tatatggaaa
     3481 tagtacaaat ggaatcctta aagaatctgg cacattaaga aaatcagtaa taaatgcaaa
     3541 gaatagagga gtattgttcg atgtatgtca tggagtggac caatttgatt ttaaagtttt
     3601 taaaaaagca ataaagcaag gtttagagcc tgacctaata agtactgata tgcatataaa
     3661 aaacatgaaa gacgtaaact attctctttt aaatgttata aataaaatta tggaacttgg
     3721 gatatcccta gaaaagtgta tagaaaaagt aactgaatac ccaacaaaga ttttcgattt
     3781 acatggaaat aaaggcagaa taaagattgg aggagaaggt gattttacaa tattcacaat
     3841 ggaggagtgt aatgatatta taaaagacta caataataat gaacttgttt taaataaaaa
     3901 attaagattg caatatactg taaaatcatg gatgaaaagc agtgaagttt atagacatgg
     3961 atacgaaaat actataataa actaaacaat agtgataaaa taataaatat atgtttaaat
     4021 tggtatatat tggataaata tctaaggaaa caacaattaa aaaataataa ttttagggga
     4081 ggtattttag tatgaaagca tttgttgata aggatgtatg tgtagcttgt ggagcatgta
     4141 taggaacatg tccagacgtt tttagtatga gcgatgaagg gcattcagta gcaattgaag
     4201 gagaaatacc agcagattta caagatttat gtaaagaagc cgaagaagga tgtccagttt
     4261 cagctataac agttaaatag agctgtttaa atttattttt aaaataaata tataagattt
     4321 aattgggtac ttaagctata gtattagaaa tttctattac tatagctttt taaaatagat
     4381 aaacttaata tgtaagatat tactttttac aaatatggag gactgaaata tgaaaaaaaa
     4441 ttatgaaata gtattattcg atttagatgg aacacttaca gaccctaaag aaggaataac
     4501 aaagtctatt cagtattctt taaatagctt tggcataaaa gaagatttgg aaaacttaga
     4561 ccaatttata ggaccaccat tacatgatac tttcaaggag tattataaat ttgaagataa
     4621 aaaagctaaa gaagctgttg aaaagtatag agaatatttt gctgataaag ggatatttga
     4681 aaataaaatt tatgagaata tgaaagagat attagaaatg ctctataaaa atggtaaaat
     4741 attattagta gcaacttcta agccaacagt ttttgcagaa actatactta gatattttga
     4801 tatagataga tattttaaat atatagcagg tagcaatctt gatggtacaa gagtgaataa
     4861 aaatgaagtt atacaatatg tactagacct atgtaatgta aaagataaag ataaagttat
     4921 tatggttgga gatagaaagt atgacataat tggagctaaa aaaataggta tagattctat
     4981 tggagtattg tatgggtatg gttcttttga agagcttagt gaaagtgaac ctacttatat
     5041 tgtcgaaaat gtagagtcta taaaagatat tttgttataa atatctttta tagacaaaaa
     5101 tattacaaaa tggtgtcgca tatttaaaaa tatgatatta tagttaagga agtcatttaa
     5161 aaaagaggag gatgcactta aaatgaaatc tattttaatt atgggtggaa gcgacttcat
     5221 aggttctgca cttgctaagc gcttgattaa atgtgggtat caaattgata tacttacaaa
     5281 tggtaaaaag gaaatagatt ataatggttt taaagaacat ctaatttgtg atagaaaagt
     5341 tcgaaaagat atggaaaaca ttatcacagg aagaaaatat gattacatat atgatatgac
     5401 agcatataca aaagaagatg taagtaatct gattgatttt ataagcatgg ataacttaaa
     5461 aaagtatata gtattgtcag caggagcagt atataaagat agtggaagaa atataaaaga
     5521 agaaaatgag aaaggtgaaa atgaaaactg gggaaaatat gggttaaata aaaaagaggc
     5581 tgaagatttt attataaata gccctatacc atatataatt attagaccaa cttacatata
     5641 tggtgaaaat aataatttat atagagagta ttatttcttt gaaaaaatag aaaaaaatga
     5701 aaagatacca gtaccaaaag gaaaacaagt tagtaatcaa tttatatata ttggggattt
     5761 ggtaaaggta ttggaaagca taatgaaaaa tcctcatgta agagaggcct ataatgtgac
     5821 aaatccacag ttaatttctt gggatgacct tatatataca tgtggagaaa ttattggcaa
     5881 agaacctata ataaaatatg tagatatgga aaaagtagag tttagagaaa gaacgtattt
     5941 cccatttaga aatatagact ttaatcttga tataaataag cttattgaac atgggcttta
     6001 tataccaaat gtgttactaa aagaaggtct tacagcaaca tataaatggt tttcagcaaa
     6061 taagccaaaa atgcatgaca gaaaaatgaa caaagttgag tctgtaatgc aaatagttta
     6121 aaaaaataaa taagtaggta gatattttga actcaaaaat taagtatttt ttaaaatttt
     6181 ttatcttaat atttattttg atttttatat actcagttgc gatagagccg agtctattaa
     6241 ttgtgaaaaa atataatata agtgaaaatg gagcattgat aaaaaatgat acaattgaga
     6301 gtaaatctga aagtgtaaaa gtagttcaaa tttcagatac tcaaataggt agtttttatt
     6361 caactaagaa tctcaaaaaa gtagctaata agattaacac tttgaatcca gatataatag
     6421 tatttactgg tgatttaatt gattactcca ataaaaatcc aagtgtagat gaaattacaa
     6481 ctattttatc aagtatgaat gctaggttgg gaaaattttc agtatttggc aatcatgatt
     6541 atatgtataa acttcctagg tattataggc agattatgaa aaattccaac ttcaatcttt
     6601 tagtaaatga aaataaaaaa ataaaattaa aagatgataa atatattaat atattaggtg
     6661 ttgatgaaat attaaatgga aatcctaata taaaatatct ggagagtcag atagataata
     6721 aaaattttaa tttattgtta gcacatgaac cagatttagt agatatgctt agtaaagata
     6781 caatgaattt agttctatct gggcatagtc atggtggtca aataagatta cctattaagg
     6841 gagcattagt tactcctcca tatgggagga aatatactaa agggttttat gatataaatg
     6901 gaaatcattt atatgtgagt tcgggtcttg gaagtacaaa gttaccattt agatttttta
     6961 atataccaga gataatagaa tttaatatta taatctaaca taaaatattt agaaaatata
     7021 attttgcttt ttataatatg tagatgtata tactgtaaaa atggatta
//
LOCUS       DAMJWP010000187         6968 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.194, whole genome shotgun sequence.
ACCESSION   DAMJWP010000187 DAMJWP010000000
VERSION     DAMJWP010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6968
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.194"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            17..709
                     /locus_tag="PC700_002961"
     CDS             17..709
                     /locus_tag="PC700_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2582237.1"
                     /translation="MYNILVVDDDKEIVDSIEIYLKSEGFKIYKAYDGMKALEIITEN
                     DIHLILMDIMMPKLDGIKATVKIREERNIPIILISAKSEDTDKIIGLNIGADDYITKP
                     FNLLELIARVKSNLRRYVTLGNYEDTCKEILKSGALELNTLTKEVKIDGENIKMTPIE
                     YKIIELLLENKGRVFSIDEIYEKVWNEESFNSENTVAVHIRRIREKIEINPKEPRFLK
                     VVWGVGYKIEKF"
     gene            905..2965
                     /locus_tag="PC700_002962"
     CDS             905..2965
                     /locus_tag="PC700_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HDF2582238.1"
                     /translation="MEKYRALKLVSLIIISISFPLTILLATIGLAEPKYNIQKTSTKS
                     GIFNISNDFYDSNIFDEGTVESLLSNFKISVDSQLTSEEKSKLQTKSKSIDLSSNDSE
                     YDKIMINKQASEEYLSYLKNIKVYVINTYTGVFYTNTSYGNLDDFKQKTKSYCNIEII
                     NNVGKSSYIKTINGEKFELNNLSMELNGVDEAFEAYVSFPEEPTIEDGIVYTNFQIFK
                     QATEKVRLFFVACLSLAIVCLLSIILYSRIKGEVLKRNNVFLLIYSKIPMEFNVILVF
                     ISIAILTMYALAVSYHIDVIVLVSLSLLIVSNNIFFINVQLSYLENKMCIFKTSIIVR
                     VILWLIKTVREVSNAIKKVSLARKVIVIVIISLALIMVISTRSIGMGLLFSICIFLGF
                     TIYITKRLSYLSYVIEGTERIKKGELDYKIKITGNDNFTSLAENINNIGEGLDRAIEE
                     QVKSERMKSDLITNVSHDLKTPLTSIINYVDLIKKEESIQPEYINDYIKVLESKSKRL
                     KLLIEDLFEASRVSSGNIELQISKIDLVQLLRQSIGELEEKLSKNNLYLKLNVPNDKV
                     YIWADGRRMYRVFENLLSNIAKYSLEETRVYIDVYDYGENVKVTMKNISSYELNFDPS
                     EIMERFKRADESRNTEGSGLGLAIARDLVALQGGKFYIEIDGDLFKANLEFQKYEEEI
                     TNME"
     gene            complement(3171..3494)
                     /locus_tag="PC700_002963"
     CDS             complement(3171..3494)
                     /locus_tag="PC700_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDF2582239.1"
                     /translation="MNNNYIFFIIIGMYIVTYLPRVLPMLIFSKKEMPDFLKKIMKFI
                     PVAILTSLTAKDVFFNGDSLYLSIANPKIISFMIVLLVAYKFKSIGISIITGVISIYL
                     FGIIL"
     gene            complement(3487..4188)
                     /locus_tag="PC700_002964"
     CDS             complement(3487..4188)
                     /locus_tag="PC700_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDF2582240.1"
                     /translation="MERTQQLKESFIASIPIFLGYIPIGIVGGILLQKSGLSPFQIAL
                     MVTLVFGGSSQFIAASMISTGASVTSIVLTTFIVNLRHFLMSSNLNMYIKNKSPKFIL
                     PFCHTITDETFAVNYEKFTAGNWSDRNAIYLNFFCLISSVLANFIGAFLGESISIDSS
                     ISGFILTSMFIALIVSQIKNKIYIVVFISSIIISVILYALFKSNLVIIVASILASLIG
                     FFIEESYCKKEESYE"
     gene            complement(4466..5851)
                     /locus_tag="PC700_002965"
     CDS             complement(4466..5851)
                     /locus_tag="PC700_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2582241.1"
                     /translation="MPINSFENYPMNWKPKRPSKGQILYKALAEQLEQDINNGFLLPG
                     TKLPPQRELADFLDVNVSTISRAFKICEKKGLISGVTGSGTFVSYDTRSNLFLMSSNN
                     KITFIEMGTMNPDFTLEEMNTLFKHIIKEIDFKTIFQYGQRDGAKWQKEAIAKLIYKA
                     GLETTSDSLLPASGGQNAIVAILAGLFQHGDRIGVDPLTYPGIKTAAKMLGIQLIPIK
                     QEDNEISEEGLLYACKNENIKGLYIIPDYQNPTTHIMSQNGRKMIANIASKYNLIVIE
                     DAIHSLLNETHLNPVASYLPNQTIYITSLSKIIAPSLRLAYISTPKQYREALSSALYN
                     INLSQSYFLTEIAYRMITSGEADKLINARRKSARRRNKIINQYLSGYNLLGNEECIFR
                     WLILPDGIMAEKFEIQALKEGVQVYASERFAVGKEKPISAIRIAVCATESTEELKAGL
                     SVLKRLLEEKK"
     gene            complement(5959..>6968)
                     /locus_tag="PC700_002966"
     CDS             complement(5959..>6968)
                     /locus_tag="PC700_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HDF2582242.1"
                     /translation="YDQGITLDIFMYIWILGIIAISFYYLRTNFKFYNYIENFKKEIL
                     EIDITSILDEQAKELSINKKLQIYKVKGIYSPAIIGLTRCKIIIPEKKYNNKELRFIF
                     KHELIHYKRKDNLFKLLISIICIIHWFNPILYLLKKYFHELCELSCDEIVIDKCTTSE
                     IKEYAYIILNTIKYQKTLKYSSFVSQFLNKKNIILKKRLNSMFNQDKKKINLGLKFLL
                     TFIIIGSIFSINSNFKQNEDVEYTIAKINYNKNGETYGTHIIDSNGNSIEPELIQTIG
                     RGKKVGYVKKRDLYDCDNQPKTPEEAVLYTRKRNFNIFKKTIPLYDKEGIKIIGRFEI
                     N"
BASE COUNT         2658 a          803 c          984 g         2523 t
ORIGIN      
        1 tagaggaagt gaaaatatgt acaatatatt agtagtagac gatgataaag aaattgttga
       61 ttcaatagaa atatatctta aatcagaagg atttaaaata tataaagcat atgatggaat
      121 gaaagctttg gaaataataa cagagaatga cattcatcta atactaatgg atataatgat
      181 gcctaaatta gatggaatca aagcaactgt aaaaatacga gaagaaagaa atattccaat
      241 aattttaatt tcagcaaaaa gtgaagatac agataaaata ataggattaa atattggagc
      301 tgatgattat ataacaaagc catttaatct attagagctt atagctaggg ttaaatcaaa
      361 cttaagacga tatgtaactt taggtaatta tgaagataca tgtaaagaaa ttttaaaaag
      421 tggagctttg gagttaaata cactaacaaa agaagttaaa attgatggtg aaaacataaa
      481 aatgacccca attgagtata aaataattga acttctttta gaaaataaag gacgagtttt
      541 ctcaatagat gagatatacg aaaaagtatg gaatgaggaa agttttaatt cagaaaatac
      601 agtagcagta catatcagac gaataagaga aaagattgaa ataaatccaa aagaaccaag
      661 atttttaaag gtggtgtggg gagttggtta taagattgaa aaattttagt ggaaaaatta
      721 taaaagaaaa gagtttaata tttaattgca ttttgatttt agtcattttt acatttacac
      781 aaatgttagg aattttaata tgttttaatt ttttctctga gagtttaatt tactctatgg
      841 ggattatgct tttcttaaac tttattttat ataaaatact gacaatgtag atgaggtggg
      901 caacttggaa aaatatagag cattaaaatt agtttcatta atcataatat ctatctcatt
      961 tccattaact atattgttgg ctacaatagg acttgctgag cctaaataca atatacaaaa
     1021 aacttctaca aagagtggga tttttaatat atctaatgat ttttatgata gtaatatatt
     1081 tgatgaagga acagtggaaa gcttattatc taattttaag atatctgtgg atagtcagtt
     1141 aactagtgaa gaaaaaagta aactccaaac aaaaagtaaa agtatagatt taagtagtaa
     1201 tgattctgag tatgataaaa tcatgattaa taaacaagct tctgaagaat atttaagtta
     1261 tctaaaaaat ataaaagtgt atgttattaa tacatataca ggagtatttt atacaaatac
     1321 atcatatggt aatttagatg attttaaaca aaaaactaag agctattgta atatagagat
     1381 aataaataat gtaggaaaga gctcgtatat taaaacaata aatggagaaa aatttgaact
     1441 gaataatctg agtatggaac taaatggagt agatgaagcc tttgaggcat atgtctcttt
     1501 tccagaagag ccaactatag aagatggaat agtatacact aattttcaaa tatttaaaca
     1561 agctacagaa aaagtaagat tattttttgt agcttgttta agtttagcaa ttgtgtgttt
     1621 attatctatc attttatata gcagaataaa aggtgaagtt ttaaaaagaa ataatgtatt
     1681 tttactaata tacagtaaga tacctatgga atttaatgta atactagtat ttatttcaat
     1741 tgcaatttta actatgtatg ctcttgctgt atcttatcat attgatgtaa ttgtattagt
     1801 ttctttatct ttactgatag tatcaaataa tatatttttt ataaatgtgc aattgagtta
     1861 tttagaaaat aaaatgtgta tatttaagac aagtattata gtaagagtga ttttatggtt
     1921 aataaaaact gtaagggagg tcagtaacgc tataaagaaa gtatctttag caaggaaagt
     1981 tattgttata gtgataatat ctttagcttt aatcatggta attagcactc gaagtatagg
     2041 tatgggatta ctgttttcta tatgtatttt tttaggattt acaatatata taactaaaag
     2101 actttcatat cttagttacg taatagaagg tacagaaaga ataaaaaagg gagaattaga
     2161 ttataagata aaaattactg ggaatgataa ttttacgtct ttagctgaaa atataaataa
     2221 tattggagaa ggtcttgata gagctataga agagcaagta aaaagtgaaa gaatgaagtc
     2281 tgatcttata acaaatgtat ctcatgattt gaaaactcca cttacttcta taataaatta
     2341 tgtagattta attaaaaaag aagaatcaat acaaccagag tatataaatg attatataaa
     2401 ggtacttgag tcaaagtcaa aaagactaaa attattgata gaagatttat ttgaagcgag
     2461 tagagtaagt agtggcaata tagaattaca aattagcaag attgatttag tgcaactatt
     2521 aagacaaagc ataggtgaac ttgaagaaaa attatctaaa aataatttat atttaaaatt
     2581 aaatgttcca aatgataagg tgtatatatg ggcagatgga agaagaatgt atagagtatt
     2641 tgaaaatctt ttatctaata tagcaaaata ttccttagaa gaaactagag tctatattga
     2701 tgtatatgac tatggtgaaa atgtaaaggt aactatgaaa aatatatctt cttatgaatt
     2761 aaattttgac ccaagtgaga ttatggaaag atttaaaaga gctgatgaat caaggaatac
     2821 tgaaggaagt ggattagggc ttgctattgc aagagattta gtagctttac aaggtggaaa
     2881 attttatata gaaatagatg gagatttatt taaagcaaat ttagagtttc aaaaatatga
     2941 agaagaaata acaaatatgg aatgagatag tatttaagta tatatatttt ttttactgtc
     3001 gaaagctttt attataatat attagaatat ttacagtaga gaatgaagag taaaaacaga
     3061 gtaactttaa gtcactctgt tttttattta aaatcaataa tataacaact taaattaaaa
     3121 ttgtacacat agttttaaaa taacttatac aataaaacat tatccaaata tcacaagata
     3181 attccaaata aatatataga tatcactcct gtaataatag aaataccaat agatttaaac
     3241 ttatatgcaa caagaagtac aatcataaaa gatataattt tagggttagc tatagataaa
     3301 tatagactat ctccattaaa aaatacatct ttagcagtta aggaagtaag tattgctaca
     3361 ggtataaatt tcatgatttt tttcaaaaaa tcaggcatct ctttttttga aaaaattaac
     3421 ataggaagca ctcttggtag atatgtaaca atgtacattc caattataat aaaaaatata
     3481 taattattat tcataagatt cctccttttt acagtaagat tcttctataa agaaaccaat
     3541 taaagatgct aaaattgatg caactattat tactaaattg cttttgaata atgcatataa
     3601 aataacagaa ataatgattg atgatataaa tactacaata taaatcttat tttttatttg
     3661 agaaactatt aaagctataa acatagaagt caatatgaag ccagaaatag aactgtctat
     3721 agatatactt tctcctaaaa atgctccaat aaaattagct aaaactgatg aaattaagca
     3781 gaagaaatta agataaatag catttctatc agaccaattt ccagcagtaa atttttcata
     3841 attaactgca aaagtttcat ctgttatagt atgacaaaat ggcaatataa attttggact
     3901 tttatttttt atgtacatgt tcaaattaga actcattaga aaatgtctta agttaactat
     3961 aaatgtagtc aaaacaatag atgttacact tgcaccagta gaaatcattg aagctgcaat
     4021 gaattgagaa cttcctccaa atactaaagt taccattagt gctatttgaa aaggtgaaag
     4081 accagatttt tgtaatagta ttcctcctac tatacctata ggtatataac ctaagaatat
     4141 aggaattgaa gcaataaagc tttcttttaa ttgttgagtt ctttccacaa taaatcactc
     4201 cttttgtatt aaatgtatta aagtattagt aaaacagaaa aatagattaa ttgtataatt
     4261 gcaattataa ttaaaaataa atttggatta atatataaaa tactatacaa caaataaata
     4321 atcttaaacg gttattaaat tttatctatt tgttattatg atgtaataat acatattaat
     4381 ctctataagt taattactat ctggtgtaat tttattttaa caacaaaaaa aaatacacac
     4441 aattaaaata ttgtgtgcaa tacaattatt ttttctcttc taatagtctt tttaatacgc
     4501 tcagaccagc tttaagttct tctgtgcttt ctgtagcaca tacagctatt cttattgcag
     4561 aaattggttt ttcttttcca acagcaaaac gttcagaagc atagacctgt acaccttctt
     4621 taagtgcttg tatctcaaat ttttctgcca ttattccatc aggtagaatc aaccatctaa
     4681 atatacattc ttcatttccc aaaagattat atccagataa atattgatta ataatcttgt
     4741 ttcttcttct tgcagattta cggcgtgcat taattaattt atcggcttca cctgatgtaa
     4801 tcatgcgata agcaatttct gttaaaaaat aagattgaga tagattaatg ttgtataatg
     4861 cgcttgataa tgcctctcga tactgtttag gtgtagagat atatgctagc cgtaaactag
     4921 gtgcaataat ttttgataaa ctcgtaatat aaattgtttg attgggcaga taggaagcca
     4981 ctggatttaa atgtgtttca ttaagtaaac tatgaatagc atcctcaata acaattaaat
     5041 tgtatttaga tgcaatattt gcaatcattt ttcgtccatt ttgtgacatt atatgtgtag
     5101 ttggattttg ataatctggt attatgtata gacctttaat attttcattt ttacaagcat
     5161 ataatagtcc ttcttcacta atctcattat cttcttgttt aattggaatt agttgaatcc
     5221 caagcatttt agctgctgtc tttattccag gataagttaa tgggtctaca cctattctat
     5281 ctccatgttg aaacaaacca gctaaaatag caacaattgc attttgtcca ccacttgcag
     5341 gaagtaagct atctgatgta gtttcaagcc cagctttata aattaatttg gcaatagctt
     5401 ctttttgcca tttagcacca tctctttgtc catattggaa tatagtttta aaatctattt
     5461 ccttaattat atgtttaaac aaagtattca tttcttctag tgtgaaatct ggattcattg
     5521 tgcccatttc aataaatgta attttattat tactggacat caagaataga tttgaacgcg
     5581 tatcataaga tacaaaagtt ccacttcctg taactccact tattagacct tttttttcac
     5641 aaattttaaa agctctagag atagtactca cattaacatc taagaaatct gccaattcac
     5701 gttggggtgg taatttagta cctggtaata aaaatccatt gttgatatct tgttctagtt
     5761 gttctgcaag cgccttatat aatatttgcc cttttgaagg tctctttggt ttccagttca
     5821 taggataatt ttcaaaagaa ttaattggca taagttttca cctcacatta ttaatttgat
     5881 tttttcttta tctaaaacaa taaattctaa agatacaaat aaaaactgat aggaaataat
     5941 tctatcagtt tttgtctttt aattgatttc aaatcttcct ataattttta taccttcttt
     6001 atcataaaga gggattgttt ttttaaatat attaaaattt ctttttcttg tatataaaac
     6061 agcttcttct ggtgtctttg gttgattatc acaatcgtac aaatctcttt tttttacgta
     6121 gccgactttt ttacctctac ctattgtttg tataagttct ggttcaatgg aatttccatt
     6181 tgagtctatt atatgtgtac cataggtttc tccattttta ttataattaa tcttagcaat
     6241 agtatattct acatcttcat tttgtttaaa attggagttg atagaaaata ttgaccctat
     6301 tattataaag gttaataaaa acttaagacc taaattaatt ttctttttat cttgattaaa
     6361 catagaattt aacctctttt ttaaaataat atttttttta tttagaaact gagatacgaa
     6421 tgatgaatac ttcaaagttt tttgatactt tattgtattt aatattatat atgcatactc
     6481 tttaatttca ctcgtggtac atttgtcaat cacaatttca tcacaagata attcacataa
     6541 ttcatgaaaa tattttttta ataagtataa aattggattg aaccaatgta tgatacatat
     6601 tatactaatt agaagcttaa ataagttatc ttttctttta taatgaatta attcatgttt
     6661 aaagataaat cttaactctt tattattgta ctttttttct ggaattatta ttttacagcg
     6721 tgttaaacct ataatggcag gggaataaat acctttaacc ttatatatct gaagtttttt
     6781 atttatactt agttcttttg cttgttcatc taagatacta gtaatatcta tttctaaaat
     6841 ttctttttta aaattttcta tgtaattata aaatttaaaa tttgttctta aatagtaaaa
     6901 agagattgct ataattccta atatccagat gtacataaat atatctaaag taatgccctg
     6961 gtcatatg
//
LOCUS       DAMJWP010000188         6938 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.195, whole genome shotgun sequence.
ACCESSION   DAMJWP010000188 DAMJWP010000000
VERSION     DAMJWP010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6938
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.195"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            106..1887
                     /locus_tag="PC700_002967"
     CDS             106..1887
                     /locus_tag="PC700_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HDF2582243.1"
                     /translation="MSQYININSQLDALKLKEFEKIFYENINEDVKVVPKVTPFKGIN
                     TDLLYVKDGRILFIKFMDTTEDIFFILEEELLEVMNEEYELLKLKMGQKNRNISYNYV
                     YIMPYVEVEETYEFEEFVNNNIIDKNKLQDIMNKGSLEEYLNDENDEINLNLFLLDIC
                     SEYYIINDKLHLNEKFKKISFYNDDYKYTATMMEDVQIKDVVSIKYGNTLIEGGSGIG
                     KTAIMLSRAIKLAKVYPHHKLVIFTHTKQLCNELRERIELLYKDNNNLEVHTFSSFIF
                     KLAKKFNLIVDYNMLKNDYEKTFNNLVKQAQNIIKNKNMFKAIFIDEAESFLEYEIDF
                     IREFLYKTKFIFNVCSCNSLNISNRLNIFKKLYNGIEFDDKIILSKNYRQAKEIVDFT
                     NKFSNNSNSYINELRPNTEFSTFFYTKALRGGNKSVDIIKVSDLDDQISSVIWEIEYL
                     ISKKGLDYSEVAIVYPYNKKKLKSGKTIYFQYMLKKALEEAKIPYICAEDNLTNISKK
                     VGTTIANIYAIKNLEYKAVIVCELEMLYNQTINDIEQDYQVNDFVGDLNKVYLAMSRA
                     TDYLSIVTTFNEEASDIIRLITESKDI"
     gene            complement(1969..2499)
                     /gene="lepB"
                     /locus_tag="PC700_002968"
     CDS             complement(1969..2499)
                     /gene="lepB"
                     /locus_tag="PC700_002968"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDF2582244.1"
                     /translation="MSVKKEIFDWIKSIAMAIVLAFVILQFIRPSVVSGESMYPTLDD
                     KDYLILNRISYKVGKPEKGDIVVFKTNLVDGETGKKKDLIKRVIATEGDRIKISNSKV
                     YVNGKLLNEPYIHNNYTSGDIDTVVPKGKLFAMGDNRENSNDSRFPDVGMVDEDEILG
                     KVMVRLLPLDNIGKVD"
     gene            complement(2539..3363)
                     /locus_tag="PC700_002969"
     CDS             complement(2539..3363)
                     /locus_tag="PC700_002969"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDF2582245.1"
                     /translation="METFSLLEIFKAVILGIVQGITEWLPVSSTGHMILINEFIKLNF
                     SNTFINTFLVVIQFGSILAVLVIFFRKLNPFDSAKNIKQKKETVRLWLKVIIAVIPSG
                     VIGILFEDDIDRVFFNSTVVAIALIVYGIIMIGLEKRNKRPKYKDFSQVTYKLALCIG
                     LFQCLALIPGTSRSGSTIIGAVLLGTSRYVAAEFSFFLAIPTMLGASALKLLKAGFGF
                     TGFEWLILGVGSVVAFVVSIVVIKFFMDYIKKHDFKVFGYYRIVLGIVVLAYFFLL"
     gene            3620..4528
                     /locus_tag="PC700_002970"
     CDS             3620..4528
                     /locus_tag="PC700_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2582246.1"
                     /translation="MYSTKILDDYEYETFNVFSTKLNPNTKHDYLSKVILFKEFLKGK
                     ELIYATKEDCKNFVDYIQTKYAKSTCEKIYSYLHSFYNFLKKEGYIDINPFRYVEKPT
                     VTRIKTKDDVLSIQEINKLIGILPKLNIRDRVIIVCLVTTGCLLNELVSLKWKDLMVD
                     ENDNSYVRLGKGRKERVVKLHPYFFKLLEDYRNYSGLPEVIIPSDDFIFTTQKSNSIT
                     DRNVRLIVKKALDLAGLSQYSARDFRHSFAAISLRLGADESDVKNQLGWSDKYYAIRY
                     KYVLNFVDSEIVDYLIEKDNLDINKK"
     gene            4672..6117
                     /locus_tag="PC700_002971"
     CDS             4672..6117
                     /locus_tag="PC700_002971"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDF2582247.1"
                     /translation="MIDNKYTTYSNPLTDRYCSKDMSYIFSPQFKFSTWRRLWVALAE
                     GEHELGINITEEQLDELRANIDNINFDEARKIEKEVRHDVMSHVKAYGLQCEKAKGII
                     HLGATSAYVGDNTDVIQMNEALKLLRVKLVNLINNLKEFALKYKDMPALGFTHFQAAQ
                     LTTVGKRACLWAQDLILDLEDLNYRIDNMKLRGVKGTTGTQASFLSLFEGDHEKVKEL
                     DKKICKKMGYHSSYAVSGQTYTRKLDYQVISILSGIAQSMHKMTNDIRLLQSLKELEE
                     PFEKNQIGSSAMAYKRNPMRSERIASLSKYIISESISPAMVQATQWLERTLDDSANKR
                     LAIPQAFMAADAILEIGINVTDGLVVYENMINKRVNEELPFMATETILMEAVKRGGDR
                     QELHEIIREYSMKAAYRVKHEGKDNNLIELIMNDDSFKMSKEEILSIMDPKNFIGRAP
                     EQVVEFVNEVVEPAIKDYKEDLGKVDVDLRV"
     gene            6428..6880
                     /locus_tag="PC700_002972"
     CDS             6428..6880
                     /locus_tag="PC700_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2582248.1"
                     /translation="MNYIVKDTISFLVKTFPKIYSSLYLEDLKKFAPDYNVNKTQLRA
                     LVFIKNYGVISMTDLCSKLNIEKGSLTSMVDDLTDKKYVTRKRDLVDRRKYLIDITEE
                     GDKIATDFMDKLSDGLGEKLSKLTEEDRKKYLEAINTLQYILNKEEFR"
BASE COUNT         2852 a          825 c         1043 g         2218 t
ORIGIN      
        1 tttgtattta ttttgaattt atttactaaa tttgcctata aaaagtatat ataaaaatat
       61 agttcaaaat ggtataatta agtataacag aaggaggtaa tgtaagtgag ccaatacata
      121 aatataaatt ctcagttaga cgccctaaaa ttgaaggagt tcgagaaaat attttacgaa
      181 aatatcaatg aggacgttaa agtagtgcca aaagttactc cttttaaagg aataaatacc
      241 gatttgttgt atgttaaaga tggaagaatt ttatttataa aatttatgga tacaacagaa
      301 gatatatttt ttattttaga agaagagtta cttgaagtta tgaatgagga atatgaactt
      361 ttgaaactaa aaatgggaca aaagaataga aatataagtt ataactacgt ttacattatg
      421 ccatacgttg aagttgagga aacatatgaa tttgaagaat ttgttaataa taatataata
      481 gataaaaata agcttcaaga tataatgaat aagggttcgc tggaggagta tcttaatgat
      541 gagaatgatg aaataaactt aaatttattt ttactagaca tatgttcaga atactatata
      601 ataaatgata aacttcatct aaatgaaaaa tttaaaaaaa tttcattcta caatgatgac
      661 tataagtata cagcaactat gatggaagac gttcaaatta aagatgttgt ttctattaaa
      721 tatgggaata cacttattga aggtggttct ggtataggca aaacagcaat tatgctttct
      781 agggctataa aattagcaaa agtatatcca catcataaat tagtcatatt tacacataca
      841 aaacaattgt gcaatgagct tcgagagagg atagagttat tatacaaaga taataacaat
      901 ttagaagttc acacatttag ttcttttata tttaaactag ctaaaaaatt caatttaatt
      961 gtagattata acatgttaaa aaatgattac gaaaagacct ttaataatct tgtaaaacaa
     1021 gcacagaaca ttataaaaaa taaaaatatg ttcaaggcta tttttattga tgaagcagaa
     1081 agctttttag agtatgaaat agattttata agggagtttt tatataaaac aaaattcata
     1141 tttaatgtgt gttcttgtaa ctccttgaat atctctaata gattgaatat atttaaaaaa
     1201 ttatataatg gtatagaatt tgatgataag attatcttaa gtaaaaatta tagacaagct
     1261 aaagagattg tagattttac aaataaattt tcaaataatt ctaactcata tatcaacgaa
     1321 ttaagaccaa atacagaatt tagtactttc ttttatacta aagcacttag aggtggaaat
     1381 aagtctgttg atataataaa agtaagtgac cttgatgacc aaataagttc tgtaatctgg
     1441 gaaattgagt atttaataag taaaaaaggt cttgattatt ctgaagttgc tatcgtttat
     1501 ccttataata agaagaaatt aaaaagtgga aaaaccattt actttcaata tatgttaaaa
     1561 aaagcattgg aagaggcaaa aattccttat atatgtgcag aagataattt gacaaatata
     1621 agtaagaagg taggaactac aatagcaaat atatatgcaa taaagaattt agagtataaa
     1681 gcggtaatag tatgtgaatt agagatgtta tacaatcaaa ctataaatga tattgaacaa
     1741 gattatcaag taaatgactt tgtaggagat ttaaataagg tttatttagc tatgagtaga
     1801 gcaacagact acctaagtat agtaacaact tttaatgaag aagcaagtga tataataaga
     1861 cttattacag aatcaaaaga tatttagtaa aaaaatattt gtaatttaag aaaataaaaa
     1921 gagactgaga tttgtaaaat aaactttacc aattcagtct cttatttttt agtccacttt
     1981 cccaatatta tcaagaggta atagtctcac cataacctta ccaagaattt catcttcatc
     2041 aaccatacct acatcaggga atctactatc attactattt tctctattat ctcccattgc
     2101 aaatagttta ccttttggaa caacagtatc tatatctcca gaagtatagt tattgtgtat
     2161 atatggttca tttaataatt ttccatttac atacacttta gaatttgata tttttattct
     2221 gtcaccttca gtagctataa ctctttttat taagtctttt ttctttcctg tttctccatc
     2281 aactaaattg gttttaaaaa ctacaatatc gcctttttca ggtttaccaa ccttgtatga
     2341 tatcctattt aaaatcagat aatctttatc atctaaagta ggatacattg attctccact
     2401 tacaacagaa ggtcttataa attgtagaat tacaaatgca agtacaatag ccatagctat
     2461 tgacttaatc caatcaaata tttctttttt aacactcata acttttctcc ttatcatttg
     2521 ttattaaatt agaatacatt ataatagaaa gaaataagca agaaccacta tacccaagac
     2581 tattctataa taaccaaata ctttgaagtc atgttttttg atatagtcca taaaaaattt
     2641 aataactaca attgaaacaa caaatgctac tacagaacca acacctaaaa taagccattc
     2701 aaatccagta aatccaaatc cagcttttag aagttttaat gcactagctc caagcattgt
     2761 aggaatggct aagaagaatg aaaactctgc tgctacatat ctagaagtac caagtaatac
     2821 tgcacctatt atagtagaac cagacctaga tgtacctggt atgagagcta gacactggaa
     2881 taatcctata caaagagcta atttatatgt aacttgagaa aaatctttat acttaggtct
     2941 tttatttctc ttttcaagcc ctatcattat tataccatag acaataagag caatagcaac
     3001 tactgttgaa ttaaagaata ctctgtctat gtcatcttca aacagtattc caataactcc
     3061 agatggtata actgcaatta taaccttaag ccataaacga actgtttctt ttttctgttt
     3121 aatattcttt gcactatcaa aaggatttaa ctttctaaaa aatataacaa gtacagctag
     3181 tattgaacca aattgaatta caactaaaaa tgtatttata aatgtatttg aaaaattcaa
     3241 ttttataaac tcattaatta atatcatatg ccctgtacta cttactggta gccattctgt
     3301 tattccctga actatgccca aaatgactgc cttaaaaatt tcaagcaaac taaatgtttc
     3361 caaatcaaaa tacctccaat acaaatttat agtagcatat tttattcata gattaataaa
     3421 aaaatgccac cttttactaa cttataatac catagtactg tgtagtgcac aatggaagat
     3481 tgatattttc ataaaacctt cataatataa caaaataaac ttaaaagtta tctattgaat
     3541 aaggggtata tttgaggggg aaatatatgg tatagtaaaa gtatagctat tttattttat
     3601 gaaaaggaaa ggagttatta tgtatagtac aaaaatacta gatgattatg agtatgaaac
     3661 ttttaatgtt ttttctacaa aattaaatcc aaacacgaaa cacgattatt tatcaaaggt
     3721 tatactgttt aaagaatttt taaaagggaa agaattgatt tatgctacaa aagaagattg
     3781 taaaaatttt gtagactata tacagactaa atatgcgaaa tcaacttgtg aaaagatata
     3841 tagttattta catagttttt ataacttctt aaaaaaagaa ggatacatag atataaatcc
     3901 atttagatat gtagaaaaac ccacagtaac aagaataaaa acaaaagatg atgttctaag
     3961 tatacaagaa ataaataaac ttataggtat cctaccaaaa ttaaatataa gagatagagt
     4021 aataatagtc tgtctagtta ctactgggtg tttattaaat gaattagtta gcttaaagtg
     4081 gaaagacctt atggtagatg aaaacgataa ctcatatgta aggttaggta agggaagaaa
     4141 agaaagagta gttaaattac atccatactt ttttaaactt ttagaagatt acaggaatta
     4201 ttctgggctt ccagaggtaa taatacctag tgatgatttt atttttacaa ctcaaaagag
     4261 taattctata acagatagaa atgtcagact tatagtcaaa aaagcattag atttagctgg
     4321 actttctcaa tattcagcaa gagattttag acattcattt gcagcaataa gtttaaggct
     4381 tggggcagat gaaagtgatg taaaaaatca gcttggatgg agtgataaat actatgcaat
     4441 tagatataag tatgtattaa attttgttga tagtgaaata gtggattact taattgaaaa
     4501 agataatttg gatataaata aaaaatagta ataaataaaa aatactatat atagatgtga
     4561 acaaaaaata aaaaagtctt gttatagttc gtaaaattat atataattat ttatgtacta
     4621 atgtgtacac agtaatcaca cgacaaaaga tttaaggaag gtgctaatat aatgatagat
     4681 aataaatata ctacttactc aaacccactt acagacagat attgtagcaa ggacatgagt
     4741 tatatatttt ctccacaatt taaattctct acatggagaa ggttatgggt tgcacttgca
     4801 gaaggtgaac atgagttagg aatcaatata actgaagaac aattagatga gctaagagct
     4861 aatatagata atataaattt tgatgaagct agaaaaatag aaaaagaagt aagacatgat
     4921 gtaatgtctc acgtaaaagc ttatggttta caatgtgaaa aagccaaagg aataatacat
     4981 ttaggagcaa catctgcata tgttggagat aatacagatg ttatacaaat gaatgaagct
     5041 ttaaaacttc taagagtaaa gcttgtaaat ttaataaaca atttaaaaga atttgcactt
     5101 aagtacaaag atatgccagc tcttggattt acacacttcc aagctgctca acttacaaca
     5161 gtaggaaaaa gagcttgttt atgggctcaa gatttaattc ttgatttgga agatttaaac
     5221 tatagaatag ataacatgaa gttaagagga gttaaaggta ctactggtac tcaagcaagt
     5281 tttttaagct tatttgaagg agaccacgaa aaagtaaaag agttagataa gaaaatttgt
     5341 aaaaagatgg gataccattc ttcctatgca gtaagtggac agacatatac tagaaagtta
     5401 gattatcaag taataagcat actatcaggg atagctcaaa gtatgcataa gatgactaac
     5461 gatataagac ttttacaaag tttaaaagaa ttagaagagc catttgaaaa aaatcaaata
     5521 ggttcatcag caatggctta taagagaaac ccaatgagaa gtgagagaat agcatcttta
     5581 tctaagtata taatctctga atctataagc cctgcaatgg tacaagccac tcaatggtta
     5641 gagagaacat tagatgactc agccaataag agattggcaa tacctcaagc atttatggca
     5701 gcagatgcta tacttgaaat agggataaat gtaactgatg gtttagtagt atatgaaaat
     5761 atgataaata aacgtgtaaa tgaagaactt ccttttatgg ctacagaaac aatacttatg
     5821 gaagctgtaa aaagaggtgg agatagacag gaattacatg aaataataag agaatattct
     5881 atgaaagctg cctatagagt aaagcacgaa ggtaaggata ataacttaat tgaacttata
     5941 atgaatgatg attcatttaa aatgagcaag gaagaaatac tatctataat ggacccaaaa
     6001 aactttatag gtagagctcc agaacaagtt gtagaatttg taaatgaagt tgtagaacct
     6061 gctataaaag attataagga agacttagga aaagtagatg tggacctaag agtatagaat
     6121 tgtacagata aaatataatt aaaaacggct atcttattta taaataagat agccatttta
     6181 atgggctagc gtcttctctt cagcgctgtg aagagttctt ctttacttac tataccatga
     6241 atttaataaa atagctatat aaataatatt taatagaaat aatataaata aaaataaaaa
     6301 aatgataaga ttactaatta aatttaaaca ttcagcaaaa actattgtac ttgcaatatt
     6361 tttaatgtaa actgaaataa agttatttag ataatttaat aaattttaaa acaaccgagg
     6421 tgattatatg aactatatag taaaagatac cataagtttt ttagtaaaaa catttcctaa
     6481 gatttattct agtctgtact tagaagacct aaaaaaattt gcaccagatt ataatgtaaa
     6541 taaaacacaa ttaagagcat tagttttcat aaaaaattat ggagtaataa gtatgactga
     6601 tttatgctct aagttaaata tagaaaaagg cagtttaact agtatggttg atgatttaac
     6661 tgataagaag tatgtaacaa ggaaaagaga tttagttgat agaagaaaat atctaattga
     6721 cattactgaa gagggagata agatagcaac tgattttatg gataaattaa gtgatggttt
     6781 aggagaaaaa ctttctaaat taactgaaga agacagaaaa aaatatttag aagcaataaa
     6841 tactcttcaa tatatattaa acaaagaaga atttagatag aaaaaatata aaaaattttt
     6901 atatttaata gtactaagat taattctttg ttattcat
//
LOCUS       DAMJWP010000189         6923 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.196, whole genome shotgun sequence.
ACCESSION   DAMJWP010000189 DAMJWP010000000
VERSION     DAMJWP010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6923
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.196"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(322..1218)
                     /locus_tag="PC700_002973"
     CDS             complement(322..1218)
                     /locus_tag="PC700_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2582249.1"
                     /translation="MKDKNTYFNRKGQENFIDIFLKDDIKKGANFKRHWHEHLQIYYF
                     IDGIAYLECGLDNFKVVPGNIVIANSNELHYLESLSDNLKFYSIRIEPTFLFSNQVDL
                     LQTKYLAPLALNHIVFNNLIQNDFLILKCITTILDEYHNKEVGYELSIKANIYQLIVL
                     LLRSHIDKIYSEDEFIKRKYSLEKFECIFDLIEEKYMDKITLKELASRVNLSAHHFCR
                     TFKQITGKTTIDFINGIRLDKAIYYLKETDLNMTEIAMRCGFDSINYFSRLFKRNYNI
                     SPTKYRNEHREVSNVILKEDMK"
     gene            1360..2787
                     /locus_tag="PC700_002974"
     CDS             1360..2787
                     /locus_tag="PC700_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2582250.1"
                     /translation="MTNSFKKDDNRKKITVLFVVIAMTFMATLDSSIINVALPVLASK
                     LNVSLASIEWVIASYSIIICSTLLFFGRLGDIIGKSRVFQVGTILFTSASLLCGLSNS
                     LTLLIVCRFIQGIGASAYMANNHGIITELFPKESRGKALGILVTAVAIGNMVGPSVGG
                     FILSIFDWNVIFFINIPIGLIVIFLNTKFLPNSKKSSENMDKTGAILQFLGTTLFFSA
                     LISAQQTGLLNPYILIALLLSIIFIILFLILEKKHPQPLLDLEIFRNFKFSLNLICAL
                     TSFICIASSSILIPFYLQSTMKLPPIQAGLFMILSPLILAIFSPIFGNISDKIKSEKI
                     ILIGLLVMSFGFFLMSRLKESSALILFVIYISIISIGQAIFQPANNALIMSSCSRSKL
                     GVVGSINSLVRNLGQVIGITISTTLLYNFMSIKAGYRVNDYVINNDKIFVFGMRNVYI
                     IVTLVCLIGAILIGFYLFKYNKNEQ"
     gene            complement(2984..5578)
                     /gene="clpB"
                     /locus_tag="PC700_002975"
     CDS             complement(2984..5578)
                     /gene="clpB"
                     /locus_tag="PC700_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDF2582251.1"
                     /translation="MDVEKMTLRVQKSLNEAYNEAVKNHNQQVDVIHLFSALINQEDG
                     LIPNIIEKMNISIDSLRNTVNFEIDKLPKVYGEGADSQGVSATRKINEVLIKAESISK
                     EFKDSYISVEHVMLAMMETESKSAVGKILKQYNINKNDFLNILSQVRGSQRVETQDPE
                     GTYDALARYGTNLVDLAKKNKLDPVIGRDEEIRRIIRILSRRTKNNPVLIGEPGVGKT
                     AIVEGLAERIVRGDVPEGLKDKIVFSLDMGALIAGAKYRGEFEERLKAVLKEVQSSDG
                     KIILFIDEIHTIVGAGKTEGSMDAGNLIKPMLARGELNCIGATTFDEYRKYIEKDKAL
                     ERRFQPVIAEEPTVEDTISILRGLKERFEIHHGVRIHDNAIVAAAKLSHRYIQDRFLP
                     DKAIDLIDEAGAMIRSEIDSLPTELDVVRRKLFTLETEREALLKENDDKSKSRLDDIQ
                     KEIAELKSKNDEMTAKYEKEKSQILDIKNLKAQLDEAKGKAEKYEREYDFNKAAEVKY
                     GEIPKLEEQIKQYEENMNDGSENSLLKEEVTEEEISSIVSKWTGIPVTKLVEGEREKL
                     LKLEDELHKRVIGQDEAVTAVSNAVIRARAGLKDERKPIGSFIFLGPTGVGKTELAKT
                     LARNLFDSDDNIIRIDMSEYMEKHAVSRLVGPPPGYVGYEEGGQLTEAVRRAPYSVIL
                     FDEIEKAHEDVFNMFLQILDDGRLTDNKGKTVDFKNTLIIMTSNIGSNYLLEAGGNIT
                     ETTNNLVMNEMKHRFKPEFLNRVDDIIMFKPLDQENIKKIIDIFMKDLKNRLKEKDIT
                     IEVTNSAKDVMVREGYDPVYGARPLKRYIGNTLETIIAKKLIAGDVYNGCTIVIDGKD
                     ENIEVLVK"
     gene            complement(5615..6211)
                     /locus_tag="PC700_002976"
     CDS             complement(5615..6211)
                     /locus_tag="PC700_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582252.1"
                     /translation="MSLKLDRNVLQWFDYVFENEKTSLRHYNFNCTLKEISSTSLNKV
                     AFILEKNNSKYWKLYFEIPAEVTLKLKQNIHPLFREYIYEQISLYNNNQIYNFVNSNI
                     LKVFNNIAIYQYNILENLYTIDFKKSFIDKCQYLLIGEKRLIDEDLYLIAKSKEVFDF
                     FNSDGTFNLTLSFDIQKNENLLDSLLELRKSIIINERI"
     gene            complement(6228..6632)
                     /locus_tag="PC700_002977"
     CDS             complement(6228..6632)
                     /locus_tag="PC700_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HDF2582253.1"
                     /translation="MDKTLKKDLNQEYTESKSLVMNKSKYMNICFKNYIKVVEGMYTK
                     RDLIPLKLINSMDKNNKIRKTLYELSCEFEYPKTSLSSLFTELKKMDFLQRVKNGEYM
                     INPHIAYKGSRADKESLLAEYNQIKRPNKVSK"
BASE COUNT         2221 a         1113 c          726 g         2863 t
ORIGIN      
        1 tctataaaaa tcatactatt ttagctgttt acttgcttat ttgatagttt tatttctttg
       61 attaaatatc tttcagaatc taagccagat gtctcaaaaa aactgattta taatataatc
      121 attttttctg ctattcttat ttttccaatt ttagtttcat attaaaatat acttttcata
      181 gacactacat tctttcatac tcattttcac atttaactat acaagtagtc ttactcattt
      241 atgtactata ctttattaat atttattaag aaaaactcaa agatttaaaa atatatattt
      301 tctataaagt atatttatga attatttcat atcttctttt aaaataacat tactaacttc
      361 tctatgttca tttctatact tagttggaga tatattataa ttccttttaa ataagcgact
      421 aaaataattt atgctatcaa agccacatct catagcaatt tctgtcatat ttaaatctgt
      481 ttcctttaaa taatatatag ctttatcaag cctgattcca tttataaaat cgattgtagt
      541 cttacctgtt atttgcttaa atgttctaca aaagtgatgt gcactcaagt tcacacgact
      601 agcaagttct tttaatgtaa ttttatccat atatttttct tctatcaagt caaagataca
      661 ttcaaatttt tctaaagaat actttctttt tataaattca tcttcagaat atattttatc
      721 aatatgactt cttaataaga gtactattaa ttgatagata ttagctttta tagataactc
      781 atatccaact tctttattgt gatattcatc aagtatagtt gtaatacatt ttaaaatcag
      841 aaaatcattt tgaattaaat tattaaatac aatatgattt aaagccaatg gtgctaagta
      901 cttagtttgt aacaaatcaa cttgattact aaaaaggaat gtgggctcaa tacgaatact
      961 atagaatttt aaattatctg ataagctttc taaataatgt aattcattac tgtttgctat
     1021 tacaatattt ccaggcacta ccttaaaatt atccaatcca cattcaagat atgctatacc
     1081 atcaataaaa taatatattt gtagatgctc atgccaatgt cttttaaagt ttgctccttt
     1141 tttaatgtca tcctttaaaa aaatatctat aaagttttct tgacctttcc tattaaaata
     1201 tgtattttta tccttcatta ttcccccctc atcaaaatag tgctaattat aagcaatata
     1261 gttattgtat tcattatttc atatcgctat tatataaaat gtaactatca ttgtaaacat
     1321 taacataatg ccagtcatta taataaggag gtaataaaaa tgacaaactc ttttaaaaaa
     1381 gatgataata gaaaaaaaat cacagtttta tttgtagtca ttgccatgac ttttatggca
     1441 acattagatt cgagtattat aaatgttgct ctcccagttc ttgcaagcaa actaaatgta
     1501 tctctagcat ctattgaatg ggtaatagca agttactcaa ttattatttg ttcaacacta
     1561 ttattttttg gaagacttgg agatattatt ggtaaatcaa gagtattcca agtaggtact
     1621 attctcttta catcagcttc attactatgt ggattaagta attctttaac tttacttata
     1681 gtttgccgct ttattcaagg aataggcgct tctgcctaca tggcaaacaa tcatggaatt
     1741 atcacagaac tatttccaaa agaaagcaga ggaaaagccc tagggattct agttactgca
     1801 gttgctattg gaaatatggt tggaccatct gtgggaggat ttattttgtc aatatttgat
     1861 tggaatgtta tattttttat aaatattcca atcggtctta ttgtaatctt tttaaataca
     1921 aaatttcttc ctaatagtaa aaaatcttca gaaaatatgg ataaaactgg tgctatactt
     1981 caatttttag gaacaacatt attttttagt gctctaatta gtgctcaaca aacaggatta
     2041 ctaaatccat atatattaat tgcactatta ttatcaatta tttttattat attatttttg
     2101 atattagaaa aaaaacatcc tcaaccgctt cttgatcttg aaatttttag aaattttaaa
     2161 ttttcattga atttaatttg tgctcttaca tcattcattt gtattgcatc atcaagtatt
     2221 ttaattccat tttacttaca aagtactatg aaacttcccc ctattcaagc aggtttattt
     2281 atgatactat cacctttgat tttagccatt ttttcaccta tatttgggaa tatatcagat
     2341 aaaatcaagt cagaaaaaat tattttaatt ggtcttctcg ttatgagttt tggattcttt
     2401 ctaatgtcaa gattgaaaga aagttctgcc cttattttat ttgtaattta tatatcgata
     2461 atatcaatag gacaagctat atttcaacct gctaacaatg ctttgataat gtcaagctgt
     2521 tctagaagta agctaggagt tgtaggaagc ataaattccc ttgtaagaaa tttaggacaa
     2581 gttataggta ttactatttc tacaacactt ttatataatt ttatgagtat taaagctgga
     2641 tatagggtca atgattatgt gattaataat gataaaatat ttgtatttgg aatgagaaat
     2701 gtttatatta ttgttacact tgtttgctta ataggtgcaa tattaattgg cttttattta
     2761 tttaaatata ataaaaatga acagtagttt tttataatta tattcaaatt atgattatat
     2821 tataagaaac ttactaaaat acttcctttg agttaaattt aatttggaca tattgactta
     2881 aaaatgtttt attaatttga taaaatattt cttttcctat agtttataaa taaaaatcag
     2941 ctgagcaaaa tatttagtat attatgctca actgattctt ttattattta actaaaactt
     3001 caatattttc atcttttcca tctataacta tagtacatcc attatacaca tctccagcaa
     3061 taagtttctt tgcaattata gtttctaatg tatttcctat atatctctta agaggtcttg
     3121 ctccatatac agggtcatat ccttctctca ccataacatc ttttgctgaa tttgtaactt
     3181 caatagtaat atctttttct tttaatctat tttttaaatc tttcataaat atatctataa
     3241 ttttcttgat attttcttgg tctaaaggct taaacattat aatatcatct actctgttta
     3301 aaaactcagg tttaaatcta tgcttcattt cattcattac taaattgtta gtagtctctg
     3361 ttatatttcc tcctgcttct aatagataat tacttcctat attagaagtc attattataa
     3421 gagtattttt aaagtctact gtcttacctt tattatcagt aagtctacca tcatctaata
     3481 tctgtaagaa catgttaaat acatcctcat gtgctttttc tatctcatca aatagtatta
     3541 cagaataagg agcacgtcta actgcttctg taagttgacc accctcttca tatcccacgt
     3601 accctggagg aggtccaact agtcttgata cagcatgttt ttccatgtat tcagacatat
     3661 ctattcttat tatattatcg tcactatcga ataaatttct agcaagagtt tttgcaagtt
     3721 cagttttacc aacacctgta gggcctaaga aaataaatga accaattggt tttctttcat
     3781 cttttaatcc tgctcttgca cgtattactg catttgaaac tgctgtaact gcttcatctt
     3841 gtccaataac tcttttatga agttcatctt ctagtttaag taatttttct ctttctcctt
     3901 caactagttt agttactggt atacctgtcc actttgatac aatgctagat atttcttctt
     3961 cagtaacttc ttcttttaaa agagaatttt cagaaccatc attcatattt tcttcgtact
     4021 gctttatttg ttcttctaac ttaggtattt caccatattt aacctctgct gctttgttga
     4081 aatcatattc tctttcatac ttttcagctt tacctttagc ttcgtctaat tgagccttta
     4141 agtttttaat atctaatatt tgactctttt ctttttcata ttttgctgtc atttcatcat
     4201 ttttagattt taactctgct atttcttttt gaatatcatc caatcttgat ttgcttttat
     4261 catcattttc tttaagtaat gcttctcttt cagtttctaa tgtaaataac tttcttctaa
     4321 caacatctaa ttctgttggc aatgaatcta tttcactacg aatcatagct ccagcttcat
     4381 caattaaatc aattgcttta tctggtaaaa atctatcttg tatatatcta tgagaaagtt
     4441 ttgctgctgc tacaatagca ttatcatgta ttcttactcc atgatgtatc tcaaatcttt
     4501 cttttaaacc tctaagtata gatattgtgt cttcaacagt aggttcttct gcaattacag
     4561 gctgaaaacg tctttcaaga gccttatctt tttctatata ctttctatat tcatcaaaag
     4621 tagttgcacc aatacagttc aattcacctc ttgcaagcat aggtttaata agattacctg
     4681 catccattga accttctgtt ttaccagctc caacgatagt atgaatctca tctataaaca
     4741 gtataatctt tccatcagaa ctttgaactt cttttaaaac tgcctttaat ctttcttcaa
     4801 attcacctct atattttgca ccagcaatta aagctcccat atcaagtgag aatacaatct
     4861 tatcttttaa accttctggg acatctcctc ttactattct ctctgctaac ccttctacaa
     4921 ttgcagtttt acctacacct ggctcaccaa tcaatacagg attatttttt gttcttcttg
     4981 ataaaattct tataatcctt cttatttctt catctctccc aattacaggg tctaatttat
     5041 ttttcttggc taaatctact aagtttgttc catatctagc aagtgcatca tatgtgcctt
     5101 ctgggtcttg tgtttctact ctttgactac ctctcacttg tgataaaata tttaaaaagt
     5161 cattcttatt tatattatat tgttttaata ttttaccaac agctgatttt gattctgttt
     5221 ccatcattgc aagcataaca tgctctacac ttatatatga atctttaaat tctttagaaa
     5281 tactctctgc ctttataagc acctcattta tttttctagt agctgatact ccttgtgagt
     5341 cagcaccttc tccatatact tttggaagct tatcaatttc aaagtttaca gtatttctca
     5401 aagaatcaat tgatatattc attttttcga ttatatttgg tatcaatcca tcctcttgat
     5461 taataagtgc tgagaaaaga tgaattacat ctacttgttg gttatgattt tttactgctt
     5521 cgttataagc ctcatttaaa cttttttgaa ctcttaaagt cattttttct acgtccatat
     5581 aaatccctcc attttaatat tatatccaaa taaattaaat tctctcattt ataattattg
     5641 attttctaag ctctaaaaga ctatcaagta aattctcgtt tttttgtata tcgaaactca
     5701 atgttaaatt aaatgttcca tctgaattaa aaaaatcgaa tacttctttt gattttgcta
     5761 ttaaatacaa gtcttcatct ataagtcttt tttcacctat taataaatac tgacatttat
     5821 ctataaaact ctttttaaaa tcaatagtgt atagattttc taaaatatta tattgataaa
     5881 tagcaatatt attaaacact tttaatatgt ttgaatttac aaaattgtaa atttgattat
     5941 tgttatacag tgatatttgt tcgtatatat attccctaaa taaaggatga atgttttgct
     6001 taagttttaa agtaacttca gctggtattt caaaataaag cttccagtac ttagagttat
     6061 ttttttctaa aataaaagct actttgttca aactggtaga actaatctcc tttagtgtac
     6121 aattaaaatt atagtgtctc aaactagttt tttcattctc aaatacatag tcaaaccact
     6181 gtagaacatt tctatcaagt tttaaactca tataaaaacc ttcttcctta tttactaacc
     6241 ttattaggtc tttttatctg attatattct gctaataaac tttctttatc agctctacta
     6301 cctttatatg ctatatgtgg atttatcata tactcaccat ttttcactct ttgtaaaaaa
     6361 tccatttttt tcaactccgt aaacaatgaa gataaagaag tttttggata ttcaaattca
     6421 caagataatt catataatgt ttttctaatc ttattatttt tatccataga gtttattaat
     6481 tttaatggaa ttaaatccct cttagtatac attccttcta ctactttaat ataattctta
     6541 aaacaaatat tcatatactt acttttattc ataactaaac ttttgctctc tgtatattcc
     6601 tgatttaaat ccttttttaa tgtcttatcc aaattaaata tctcttctca ctctccttat
     6661 ctctaattga taatataaat atattacaaa aaaatatgta tgtcaaaact gaaaatgcaa
     6721 aaagtttacc ttataagtaa acttttgttt agctataagc taaactttat tattttttta
     6781 atttagtatt tccaatttat tggcaggatt tttgaataat atttataaat tttttaaaaa
     6841 aatttaaatc tatttaatat ggcagccttt cataaaatac attacattta ttaaatgtat
     6901 aatattggta attagttttt tct
//
LOCUS       DAMJWP010000190         6769 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.197, whole genome shotgun sequence.
ACCESSION   DAMJWP010000190 DAMJWP010000000
VERSION     DAMJWP010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6769
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.197"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..787
                     /locus_tag="PC700_002978"
     CDS             <1..787
                     /locus_tag="PC700_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2582254.1"
                     /translation="LIFEYNLRVNTITTENLFIFIFAILLILSSSIILLIVIGKIVQD
                     DKLNLEYELINERIKTNYRNYENINEVHDRLKYVYHDLKNHMICIKNYDTKEEIISYI
                     DNLEFQISEFEHFKNTGNKTLDLILAEKISICKKYDIQIEDNINISKLNFIENNDICS
                     IFANSLDNAIEACMDINNELEKRIEVKATYINKFAIIKFINTKVNDIKLIDKRIQTSK
                     DDDKIHGIGLASIKYIVNKYGGETIVNYSDNEFILKIMIPIKS"
     gene            799..939
                     /locus_tag="PC700_002979"
     CDS             799..939
                     /locus_tag="PC700_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HDF2582255.1"
                     /translation="MKKIALNLLKNISALSFGIAVLSANSASSWVAHQAKEPQALQKL
                     KK"
     gene            998..1579
                     /locus_tag="PC700_002980"
     CDS             998..1579
                     /locus_tag="PC700_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDF2582256.1"
                     /translation="MFKRLSYKFANILVNNEIVESEDFEIYRYGFETLIYFIVNISVA
                     LFIGIIFDRFIHTVIFLSCYCTLRQFTGGYHARNYKECTLTFAVIYLITIFSANNIDI
                     NKYKYLLVLLMIISILTIYKLAPLEHRNKPLSESEKKHYRKTVQKILFVIICLIILCK
                     ILNIFQQYVIYAIISIYWIAILIYIGMKVNNDQ"
     gene            1656..3434
                     /locus_tag="PC700_002981"
     CDS             1656..3434
                     /locus_tag="PC700_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582257.1"
                     /translation="MNLLNIVESIVSCIKGLARRGINMSSIKVISDFITKIIRKNILF
                     AITVIIINYHLIQRWTHISNRGISFNDYLSLSVYTCMTISILTMLNVIFYMRKSYDTV
                     KILCRNNFDYFLGISVAVTRNNFIFCCIPIIYAIAYSSLIRTLSIVTIKSTIFILVFE
                     WFLPLTILSIVISFISVIINNLILKITLSSVFLYLTSTKMLDRVIDIKEPFFKALFKS
                     LNIFEDQSYAYYSDFVGKVNNSAYVIDKIIPTLFAITLIIVAYLILNDIRKKQIKSLI
                     VIVVYSILFIGLNYISIDMSTYRDEERSYGEYLKKSNISDIKIKSHDMKIDFKSKSNI
                     KDRIVLLNTSNEELTNINMILDEIFDIKYIKVNSENIKFNKKKDLIEVNLKESLEPND
                     EVTLDIEYEGYINVLTSWNQNKYIATKDDIMLPPGSLAWYPKVNQSSCMAYKINLSSK
                     NNVYSNLPLLNKTSNFLKKDYIFEGKSNDIAIYAGEFKEEVKDDIKIIYPSDSNVNMD
                     IEEKIKFLIETRNEILGDSKASVDAKKYVDEVDNVMESIKRKKINMILVADLPSNEFL
                     YEDSKGEKISITIYHWLSGNILIIDK"
     gene            3449..4321
                     /locus_tag="PC700_002982"
     CDS             3449..4321
                     /locus_tag="PC700_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2582258.1"
                     /translation="MINKLEIRNLNFNYKNKKALDNINLTLTDGVVALLGPNGAGKST
                     LMRLLVTLYETSIGEIELNNIKYSKNNEKIKANVGYIPQDFDMYNNINGQEYLEFVAK
                     MRGVSKNDLKKHIQKVVSKVNLDKFINNKIGTYSGGVKRRLGIAQALIGDSKLIVMDE
                     PTVGLDPEQRNEFRRLLPEISKNSIVLISTHIVEDIQFNCDKLIILNQGKILYDGTIN
                     KFIDMVEVYSVVVSNDEFEYLERNIDIIDYRRVKDGVQVKYIGKNTEKLKDSLRIDTT
                     LQDAYINFLHKELK"
     gene            4322..5110
                     /locus_tag="PC700_002983"
     CDS             4322..5110
                     /locus_tag="PC700_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582259.1"
                     /translation="MCILELIKFELKKIELKHVLIFVIISILCIFITGILDYNELIFY
                     NDVPIMDTYSIAGWLGIIKISDVFIPIMSAITIIYVFSKDYLGNINEILTLYNKKKYN
                     QFIVFRWCVILAIYTVLLLISITIVYTFTSVKGFNIDKIDFVYSVKPIDVFLKTFPTL
                     LWYTTFPLMILTLTKNRFTTIAILITYTFADIFFILHMYPFVSMINVNGFYIQKMFYM
                     PNNIDVRFNELKNYFFINRGSLVVISIIIVFYIARKSTVLKKSK"
     gene            complement(5206..6039)
                     /locus_tag="PC700_002984"
     CDS             complement(5206..6039)
                     /locus_tag="PC700_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HDF2582260.1"
                     /translation="MNVENQSLSDIEIILSHRYDNGADYWTTPDKRLIKGSPFSTLDS
                     VLYLLELGVNPDDTLLKECADLIFSTWREDGRFKLYPNGGIYPCHTANAANVLCHMGY
                     ASDIRMQKTFQHFLDTQYTDGGWRCNKFSFGRGPETEYSNPLPTLNILNAFRFSNYLN
                     KESKLDKAVDFLLDHWTIKKPLGPCHYGIGTLFMQVEYPFRNYNLFLYVYVLSFYNRA
                     KKDKRFLEALKILESKMIGNKIVVERVVPKLSKLSFCKKGESSEIATTHYYEILKNLE
                     K"
     gene            complement(6107..6736)
                     /locus_tag="PC700_002985"
     CDS             complement(6107..6736)
                     /locus_tag="PC700_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HDF2582261.1"
                     /translation="MDENITKNNYNVPFDYVRKKLKELDPHTISNLSNIPFNNDLNRF
                     TLNFFDRQYIIDYPSGEVWNSNGSLCNNFELKILIVRYLINAKGIPRTNKYVTFKEIP
                     GGNVYYPNFLNRTLYRLSEVFTSQIDKYKLVMEKIGAEKVNMGDLAYRFTYMGNTSMI
                     FILWFGDDEFPPNSNILFDSNIVYYFNAEDLAVVPDTAINAILNKINQL"
BASE COUNT         2701 a          685 c          954 g         2429 t
ORIGIN      
        1 acttatattt gaatataatt taagagtcaa tacaattact acagaaaact tatttatatt
       61 tatatttgca attttattga ttttatcatc aagtataata cttttaatag taatagggaa
      121 aattgtacaa gacgataaat taaatctaga gtatgaactt ataaatgaaa gaataaaaac
      181 aaactacagg aattatgaaa atataaacga agttcatgat agattaaaat atgtttatca
      241 tgatttgaaa aaccatatga tttgtataaa aaattatgac actaaagaag aaattatatc
      301 atatatagat aacttagagt ttcagataag tgaatttgaa cattttaaaa atactggaaa
      361 taaaacatta gatttaattt tagcagaaaa aataagtatt tgtaaaaagt atgatattca
      421 aattgaagat aacataaata tatctaaatt aaactttatt gaaaataatg atatatgttc
      481 aatatttgca aattcactag ataatgcaat tgaagcatgt atggatataa ataatgagct
      541 agaaaaaaga atagaagtta aagctacata tataaataaa tttgcaataa taaaatttat
      601 aaatactaag gtaaatgata ttaaacttat tgataaacgt attcaaacta gtaaggatga
      661 tgataagata catggaatag gacttgctag tattaaatat atagtaaata aatatggtgg
      721 tgaaacaata gttaattact cagataatga gtttatatta aaaataatga taccaataaa
      781 aagttaggag agtacaaaat gaaaaaaata gcgttaaact tacttaaaaa catctcagca
      841 ttaagttttg ggatagctgt attatcagca aacagtgcaa gttcttgggt agcacatcag
      901 gcaaaagagc cacaagcttt acaaaaacta aaaaaataac attaataaaa ggacctcttc
      961 ttattaaagg agaggtttta catgttgagg aggaaatatg tttaaaagat tatcatataa
     1021 atttgccaat atattagtta ataatgagat tgtagaaagt gaagattttg aaatatatag
     1081 atatggattt gaaactttga tttatttcat agttaatatt tcggtagcat tatttatagg
     1141 aattattttt gatagattta tacatacagt tatattttta agttgttatt gcactttaag
     1201 acagttcaca ggaggttatc atgctaggaa ttataaagag tgtacattaa catttgcagt
     1261 tatttatttg ataactattt tttcagctaa taatatagat ataaataaat ataagtattt
     1321 attagtatta ttaatgatta ttagtatact aacaatttac aagttagctc ctttagaaca
     1381 tagaaacaaa cctcttagtg aaagtgagaa aaagcattat agaaagactg tgcaaaaaat
     1441 actattcgta ataatatgtt taattatttt atgtaaaata cttaatattt ttcagcaata
     1501 tgtgatttat gcaataattt ctatatattg gattgctata ttaatatata ttggaatgaa
     1561 ggttaataat gatcagtaag attataaaac attttctaaa ttaattaatt tcactaatta
     1621 atattaattt taaaaaataa tgtcaaggta aatatttgaa cttattaaat atagttgaat
     1681 ctattgtaag ttgtatcaaa ggattggcta ggaggggcat aaatatgagt agtattaagg
     1741 ttatatcaga ctttataacc aaaataatta gaaaaaatat tttatttgca ataacagtta
     1801 ttattataaa ttaccatctc atacaacgat ggacacatat atccaataga ggaatttcat
     1861 ttaatgatta cttatccttg agtgtatata cttgtatgac aattagtatt ttaaccatgt
     1921 taaatgtaat tttttatatg agaaaaagtt atgatactgt aaaaatatta tgtagaaata
     1981 attttgatta ttttttaggt atttctgttg ctgtaactag aaacaacttt atattttgtt
     2041 gcataccaat tatatatgct atagcatact caagtttaat aagaacatta tcaattgtaa
     2101 cgattaaatc aactatattt atattggtat ttgaatggtt tttaccactt actattttat
     2161 ctatagtgat atcatttatt tcagttatta taaataattt aatactaaag attaccttat
     2221 catcagtatt tttatattta acatcaacta agatgttgga tagagttatt gatattaaag
     2281 aaccattttt taaagcatta tttaaatcac ttaatatatt tgaagatcaa tcatatgctt
     2341 actattctga ttttgtaggg aaagttaata attcagcata tgttatagat aagattatac
     2401 ctacactatt tgcaattact ttaataatag tagcatatct gatattaaat gatattagaa
     2461 agaaacagat taaatcatta atagttatag tagtgtacag tattttattt attggattaa
     2521 attatatatc aatagatatg tcaacatata gagatgagga acgtagttat ggagagtatt
     2581 taaagaaatc taatattagt gatattaaaa taaaatcgca tgatatgaag attgacttta
     2641 aaagcaagtc aaatataaaa gatagaattg ttttattaaa cacatcaaat gaagagttaa
     2701 caaatattaa tatgatttta gatgaaatat ttgatataaa atatataaaa gtaaattcgg
     2761 aaaatataaa atttaacaaa aaaaaggatt taattgaagt taatttaaag gaaagtttag
     2821 agcctaatga cgaggtgaca ttagatatag agtatgaagg ctatattaat gtgttgacat
     2881 cttggaatca aaataaatat atagctacta aagatgatat tatgcttcca ccaggttctt
     2941 tagcgtggta cccaaaagta aatcaatcaa gttgtatggc ttataaaata aatttaagtt
     3001 ctaaaaataa tgtctattca aatttacctt tactgaataa gactagtaat tttttaaaaa
     3061 aagattatat atttgaagga aagtcaaatg atattgcaat atatgcaggt gagtttaaag
     3121 aagaggtaaa agatgatata aaaataatct atccatcaga tagtaatgta aatatggata
     3181 tagaggaaaa aattaaattt ttaattgaaa caagaaatga aatattaggt gatagcaaag
     3241 catctgtaga tgctaaaaaa tatgtagatg aagtagataa tgttatggaa tcaataaaaa
     3301 gaaaaaaaat taatatgata ttagttgctg acttacctag caatgaattt ttatatgaag
     3361 actcaaaggg agagaaaatt agcataacta tttatcattg gctttcagga aatatcctta
     3421 taatagataa ataataaagg aggttaatat gataaacaag ttagaaataa gaaatctaaa
     3481 ttttaattac aaaaataaaa aagctctgga taatataaat ttaaccctaa cagatggagt
     3541 agttgcatta ttaggtccta atggtgctgg aaaatcaaca ttaatgaggt tattagtgac
     3601 tctttatgaa acttcaattg gagagataga attaaataat ataaagtata gcaaaaacaa
     3661 tgagaaaatc aaggcaaatg taggatatat acctcaagat tttgatatgt ataataatat
     3721 aaatgggcaa gaatacttag agtttgtagc taaaatgaga ggtgtatcta agaatgattt
     3781 aaagaaacat atacaaaaag tagtatcaaa agttaatctg gacaagttta taaataataa
     3841 aataggtaca tactcaggtg gtgtaaagag acgactaggt atagcacagg ctttaatagg
     3901 tgattctaag ttaatagtta tggatgaacc tacagttggt cttgatccag agcaaaggaa
     3961 tgaatttaga agattgttac cagaaataag taaaaattct atagtattaa tttcaactca
     4021 catagtagaa gacatacaat ttaactgtga caaattaatt atattaaatc aaggcaaaat
     4081 attatatgat ggtacaatta ataaatttat tgatatggtt gaggtgtatt cagtagtagt
     4141 ttctaatgat gaatttgaat atttagaacg taatattgat attattgatt atagaagagt
     4201 taaagatggt gtgcaagtaa agtatatagg taaaaataca gagaaactta aggatagctt
     4261 aaggatagat acaactttac aagatgctta tataaacttt ttacataagg agttaaaata
     4321 aatgtgtata ttggagttaa taaaatttga gcttaaaaaa atagagctta aacatgtgtt
     4381 aatatttgta attatttcta tattatgcat atttattaca ggcatattag attataatga
     4441 gttaatattt tataatgatg tacctataat ggatacatat tctatagcag gatggttagg
     4501 aattataaag atatcagatg tttttatacc aataatgtca gcaatcacaa taatttatgt
     4561 gttttcaaaa gattatttgg gtaatataaa tgagatactt actttatata ataaaaaaaa
     4621 atataatcaa tttatagtat ttagatggtg tgttatatta gctatatata ctgtattatt
     4681 attaatttca attacaatag tatatacatt tacgtcagtg aaaggtttta acatagacaa
     4741 aatagatttt gtttatagtg taaagccaat agatgtgttt ttaaagacat ttcctacatt
     4801 attatggtat acaacatttc cactaatgat attaacatta acaaaaaata gatttacaac
     4861 aatagcaata ttaattacat atacatttgc tgatatattt tttattttac atatgtaccc
     4921 atttgttagt atgattaatg taaatggatt ttatattcaa aagatgtttt atatgcctaa
     4981 taatatagat gttagattta atgaattaaa gaattatttc tttataaata gaggttctct
     5041 agtagttatt tctataataa tagtttttta tatagcaaga aaatcaacgg ttttaaaaaa
     5101 gagtaaatag tttaatatat tttttataat gttatattta tatagaatag tgactttaaa
     5161 aaatttaaag ccactatttt ttaatatgtt ttatctatat aactattatt tttctaggtt
     5221 ttttaatatt tcgtaatagt gtgttgtggc tatttcactt gactcgcctt ttttacaaaa
     5281 agataattta gatagttttg gaactacacg ttcaacgaca attttattac ctatcatttt
     5341 agattcaagt atcttcaatg cttctaaaaa tctcttatcc ttttttgcac gattataaaa
     5401 tgataaaacg tagacataaa ggaataggtt ataattacgg aaaggatatt caacttgcat
     5461 aaataatgtg ccaattccat agtgacaagg gccgagtggt tttttaatag tccaatggtc
     5521 aagtagaaaa tctacagctt tgtcaagttt tgattcttta tttagataat tactaaaacg
     5581 gaatgcattt aatatattaa gagttggtag tggatttgag tattcagttt ctggacctcg
     5641 accaaaacta aatttattgc aacgccaccc accatcagta tactgtgtat ctaaaaaatg
     5701 ttgaaacgtt ttttgcattc taatgtcaga tgcatatccc atatgacaga gtacgttagc
     5761 agcatttgca gtatgacaag ggtaaatacc tccatttgga tatagtttga aacgtccatc
     5821 ttctcgccat gtgctaaata tcaagtcagc acattcttta agtaaagtat catcagggtt
     5881 cacacctaac tctaatagat aaagtacact atcaagagta gagaaaggag aaccttttat
     5941 taaacgctta tcaggtgtag tccaatagtc tgcaccatta tcatacctgt gtgataatat
     6001 tatttcaata tctgataaag attgattttc tacattcatt ttttacccca tttcatatta
     6061 tattctatta aatctatcaa aaactttatt ataatattat ttatatttat aattgattta
     6121 ttttatttaa aattgcattt atagctgtat caggaacaac tgctaaatct tctgcattga
     6181 agtaataaac tatattagaa tcaaataata tatttgaatt tggaggaaat tcatcatctc
     6241 caaaccaaag tataaatatc atagatgtat tacccatata tgtaaaccta taagctaaat
     6301 ctcccatgtt tactttttca gctccaattt tctccataac taacttatat ttgtctattt
     6361 gagatgtaaa aacttctgaa agcctatata gagtcctgtt taaaaaattt ggataataaa
     6421 cattgcctcc tggtatttct ttaaaagtta catatttatt agttctaggg atacctttag
     6481 cattgattaa atatctaact atcaagattt taagctcaaa gttattgcaa aggctaccat
     6541 tactattcca aacttctcca cttggatagt ctataatata ttgtctatca aaaaaattga
     6601 gtgtgaatct atttaaatca ttattgaaag gtatattact taaattacta attgtatgag
     6661 ggtctaattc tttgagtttt tttcgaacat agtcaaatgg aacattataa ttattttttg
     6721 ttatattttc atccataatt ttttcctcca atttaaacaa ttaaaagtg
//
LOCUS       DAMJWP010000191         6759 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.198, whole genome shotgun sequence.
ACCESSION   DAMJWP010000191 DAMJWP010000000
VERSION     DAMJWP010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6759
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.198"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     repeat_region   474..1160
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=540..568
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaat"
     gene            complement(1552..3285)
                     /locus_tag="PC700_002986"
     CDS             complement(1552..3285)
                     /locus_tag="PC700_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2582262.1"
                     /translation="MSILLKKAPKLAKHIITSFYINRDINEVLKYLCENVTWIGPGEQ
                     EFLTSFNEIKNYFYAGQDEIPSCDINNDVFETVSEDENRCMVLGRYTVRTKENAQMIL
                     EVNQRCTFEIIEDRGKLLVKHMHISNPYGEMQLDEYFPTKIGTQSYDYLQRLLKEKTE
                     VIEMITNNINGGLKGSNDDSTYSFFYVNEGLPKILGYTYDEFMEMSGGSAVGAVYPPD
                     LPKALEDCERCFAKGPTYSSEYRIRKKDGTLMWVLDSGMKSLNSDGIVKINSIITDIT
                     QLKNIESELKLERERYRIALQNITDIMFEYDIENDTLIKYQRVEIDKKIELENFETKN
                     YSKLLESGKIIHLDDIGKLLEVLHGNLRETIEIRQVNSLTKNEWRWIRVQCSVIYDSD
                     HNPIKTIGVLKDITEDKSKLESLINQAQRDPLTQLYNQRVSQNLIEEYLCSSDSKNND
                     ALLVIDIDDFKTVNDTFGHLEGNEVLVAVSKILLHNTHDKDIVARIGGDEFTIFIKSL
                     TKDLIIKITNDILNDASKIKVKDNHKITLSIGIAFTDDSTKLYKDLFSKADKALYLSK
                     ADGKNCYSTYE"
     gene            complement(3525..4370)
                     /locus_tag="PC700_002987"
     CDS             complement(3525..4370)
                     /locus_tag="PC700_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2582263.1"
                     /translation="MFKEIFLRTFPGFLIIRDSNYRIIFINDNLKNLIKSYMQDNPLG
                     MTNIEIAKKLPDNIAKFFTDSHNIQLDWEKNYPYDKISNWILEFKKDTTSYWNVLEYK
                     VDVDEKTYIITMANDITKLYEENKRNLHYSITDPLTGAYNRKYLNDRFDIFIGDYIVL
                     IDLDNFKMINDYEGHNVGDKILCDFVSLLNKELINSTSIIRLGGDEFIVIFSSDVDKN
                     YVYSQLEALRENFLKVFSKYKYLSFSYGVDTVKRNLKLTIVELDKKMYKNKEKNKKKF
                     DKNDY"
     gene            complement(4401..6635)
                     /locus_tag="PC700_002988"
     CDS             complement(4401..6635)
                     /locus_tag="PC700_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2582264.1"
                     /translation="MQNHNYIKMLNKKVEVLSKELDSRNFKLKTLNEIDFFSKIPNRE
                     FMYQYIDDCISKNIHIGIMLLNIDNFKLFNDIYGYLVGENLLITLTQNLLHFIPESKG
                     IVAYLKGEGFLIVLPNVSNKELNNMGATIIESSRELKIRLPNEKEIYSNVTFSIGSTI
                     WNGEPNMTTLILFNQVYSALKKAKSEGKNRYILFDFKDNSFYNKLENDKEFLSEISHV
                     LQTKQIEVFYQPLYNIKNNTIVGCEALARWRHPIKGLLTPDKFIPLFEQFGLITELDL
                     HLFEENCKNIRRWIDNNDVFVPICCNFSRLHFFNPNFPSMLKGITEKYSVSTSNFSIE
                     ITENILIENTNTIIQQLAELRSYGFSVSMDDFGSGYSSFGMLQNLPFDIIKIDRIFLS
                     HNLNDFKNTTILYSIINIARALGMLTVCEGVETEQHVEFIKSINCDIAQGYYYAKPME
                     CKEFEKLLSKENNSEKMFIKYSDIGKSLIEKIFDGFFIMQDMKKLRANVTEDIQWFDF
                     YNKTEITSFPKVIEHFEKHIKGKKFSILYKSITPHNTESDSLLISGEAVLSDEASEHQ
                     KYSNFYFSANCIISENKLLLAKLHLDLIKTAGYINEFESYSIPKMTNTHLDESNILSQ
                     FYSMLPVGIIRYDLFGDMIITYMNEEMFDIIGYTKEQFFGEMGGNLRAIVHPDDLDFV
                     YKKSLDMIDTNKIEPFLYRFIKRDGQIVTVMYTQCNLSAIDGRSIAQGMYVDVNKIKN
                     LTKI"
BASE COUNT         2317 a          904 c          838 g         2700 t
ORIGIN      
        1 ttttttagat agtaaacttt aaaatataga tattaattat atgaatataa taaaaaaagt
       61 actaatgagt tacactagta ctttataact acttttacat gttttaactg tataaaacag
      121 tgggtatagt tcagcaggag tgactttagt tttgaactaa aaatcaagtt caaaaagaat
      181 aaacattagt atttgaactt cactctacgt ctaaatagat tgtagttctt cttgttttta
      241 ttataccaca aattggtaca gatattcaaa aataatatat ttatgatata ataaaaatgt
      301 aaatagtttt gcagtgagcg atatttgtta caaagtaggg cttaatgctt gaaatataag
      361 gtgttgaggg tatatgataa actttatcat ttgcactact catggttcac tgcaaattta
      421 agagagttgc acatgtgtaa gtattgaaaa tgcccagttt attttggggt aaagttttat
      481 attaactaag tggtatggaa agaggttttt cagaagtata tgtacgtcta tattgtaaag
      541 ttttatatta actaagtggt atgtaaataa ggaaggtgga agtcaatatg catgggtcaa
      601 atgggtttta tattaactaa gtggtatgta aatacgaaat tagagtttga aatatatgac
      661 tacataatag ttttatatta actaagtggt atgtaaattt gtgtcacatc attgtcactt
      721 ctttagaata tcaaaagttt tatattaact aagtggtatg taaattttta agcaggtgat
      781 taaatgatta aaaaagtaga aaagttttat attaactaag tggtatgtaa ataataactt
      841 gtcaatgtag ttatatgcaa caacttctat gttttatatt aactaagtgg tatgtaaatg
      901 aattatatat tatgaaaaaa tatggagtca cagagggttt tatattaact aagtggtatg
      961 taaatttgca ggttttcctt atagtttgcc atatgttcct ggttttatat taactaagtg
     1021 gtatgtaaat tactttagca ctagtaaagg tagaattatt aaagtgtttt atattaacta
     1081 agtggtatgt aaattcgaat gtgaatggca cttctatttc tccaactttt ggttttatat
     1141 taactaagtg gtatgtaaat agtataatgt tgaaaagtta gagagtacaa tcaagaaatt
     1201 ttacattaac taaaatgtat agaataaaac atgtatttat acttaaattc tgtacctata
     1261 taaaaaagtg aactctgtca acaaagcact tttttatata gataaattat cattttattt
     1321 taagatagaa gatactaatg ctaaatgttt atcattagta tctgtatgta cataaaagtt
     1381 taatttttta tataaatttg atctttagaa aaatgagcag tatcaataaa tatattgtct
     1441 aaattttttc taggaactag ttgactagct ataagattag cttcaactct ttgattgtta
     1501 gactatgaaa ttaaatctaa aggttcattc ttggtcgtat aaatagcttt atcattcgta
     1561 tgtactataa caatttttgc catctgcttt tgatagataa agagctttat cagctttaga
     1621 aaataaatct ttatataatt tagttgaatc atcagtgaag gcaataccaa tacttaatgt
     1681 tattttatga ttgtccttta cttttatttt acttgcatca tttaaaatat cattagtaat
     1741 ttttattatt aaatcctttg ttaatgattt tataaatatt gtaaattcat ctccaccaat
     1801 tcgagcaact atgtctttat catgtgtgtt atgtaatagt atttttgata cagcaactaa
     1861 aacttcattt ccctctaaat gcccaaaagt atcatttaca gttttaaaat catcaatatc
     1921 aataactaat agagcatcat tatttttgga atcagaacta catagatact cttcaataag
     1981 attttgtgat actctttgat tatataattg tgttagtggg tcacgttgag cctgattaat
     2041 taagctttct aatttagatt tatcttcagt tatgtctttt aaaacgccta ttgttttgat
     2101 agggttatgg tcactatcat atataactga acattgaaca cgaatccatc tccattcatt
     2161 tttagttaat gaatttactt gtcttatctc aattgtttca cgaagatttc catgaagtac
     2221 ttctagtagt tttccaatat catcaaggtg aataatttta ccagattcta atagtttgga
     2281 gtaatttttt gtttcaaaat tttctaattc aatttttttg tcaatttcta cacgttgata
     2341 ttttataagg gtatcgtttt caatatcata ctcaaacatg atatctgtaa tattttgcaa
     2401 agcaatacga tatcgctctc tttctaattt tagttcagat tctatgtttt ttagttgggt
     2461 tatgtcagta ataatactat taatttttac tatgccatca ctatttaacg atttcattcc
     2521 agaatctaat acccacatta atgttccatc tttttttcgg attctatatt cagatgaata
     2581 tgtaggtcct tttgcaaaac atcgttcaca atcttctagt gctttaggta agtctggtgg
     2641 atatacagca cccactgctg aaccaccact catttccatg aactcatcat atgtgtatcc
     2701 taatattttg ggcaatccct cattgacata gaaaaatgag tatgtactat catcattgct
     2761 accttttaag ccaccattga tattatttgt aatcatttca ataacttctg ttttttcctt
     2821 tagtaatctt tgcaaataat cataactttg agttccaatt ttagtgggaa agtattcatc
     2881 aagttgcatt tcaccataag ggtttgatat gtgcatgtgc tttactaata attttcctct
     2941 atcctcaatt atctcaaatg tacaacgttg atttacctct aaaatcattt gagcattttc
     3001 tttagttcga acagtatatc ttcctaatac catacatcta ttctcatctt cacttacagt
     3061 ttcaaataca tcattattta tatcacaaga aggaatttca tcttgacctg cataaaaata
     3121 attttttatt tcattaaaag aagtgagaaa ttcctgttct cctggtccaa tccatgtaac
     3181 attttcacaa aggtatttta atacttcatt gatatctcga tttatataaa aacttgttat
     3241 aatatgtttt gctaatttgg gtgctttttt taggagtatg ctcatgatat tcctcctttc
     3301 tttttgctat tatattttaa ttatattata catcaatata tttaaattaa aagctttgtt
     3361 aaactatgat atctttgtca aataagttaa ttttaacaaa aaagaactga aacaatatat
     3421 gcttcagtac ttaatgtcaa tatcttgtat agatacatat ttataaacta atgtacaagt
     3481 ttaaaaaaag aaagataatt ttaatttatt ttaaaataac aatgttaata atcattttta
     3541 tcaaattttt tcttgttttt ctccttgttt ttatacattt ttttatccaa ttcaactatt
     3601 gtcaatttta aatttctttt tactgtatca acaccataac taaatgaaag atacttatat
     3661 ttagagaata cttttaaaaa attttctctt aaggcttcta gttgtgaata aacataattt
     3721 ttgtcaacat cactagagaa tataacaata aattcatctc ctcctaatct tataatagaa
     3781 gtagaattta ttaattcttt atttaaaagt gaaacaaaat cacataaaat cttgtctcct
     3841 acattatgac cttcatagtc atttatcatt ttgaaattat ctaagtcaat tagtacaata
     3901 taatctccta taaatatgtc aaatctatca tttaaatatt ttctattata tgctccagtt
     3961 aatgggtcag taattgagta gtgaagattt ctcttatttt cttcatagag ttttgttata
     4021 tcattggcca tagtaattat gtaggttttt tcatcaacat ctactttgta ttctaaaaca
     4081 ttccaatagc ttgtagtatc ttttttaaac tctaaaatcc aatttgaaat tttatcataa
     4141 ggataatttt tttcccaatc taattgaata ttgtgagaat ccgtaaaaaa tttagcaata
     4201 ttatcaggga gtttcttagc tatttctata ttagtcattc caagtggatt atcttgcata
     4261 taacttttaa ttaaattttt taaattatca tttataaata taattctgta gttagaatct
     4321 cttataatta gaaatcctgg aaaagttctt aaaaaaattt ctttaaacat attaatttct
     4381 ccaaaataaa atactttgta ctagattttt gttaaatttt ttattttatt tacatctaca
     4441 tacatacctt gggcaatcga ccttccatca attgcagaaa ggttacattg tgtatacata
     4501 actgtgacaa tttgaccatc tcgttttatg aatctatata aaaatggttc aattttattt
     4561 gtgtcaatca tatccaaact ttttttataa acaaagtcta aatcatctgg atgaacaatt
     4621 gctcttaaat ttccgcccat ctcaccaaaa aactgttctt ttgtataacc tataatgtca
     4681 aacatttctt cattcatata agtaataatc atatcaccaa ataaatcgta gcgaattata
     4741 cctacaggca acattgaata aaattgtgat aaaatattac tttcatcaag atgtgtattt
     4801 gtcattttag gtattgaata tgattcaaat tcatttatat agccagcagt tttaattaaa
     4861 tccaaatgta attttgctaa gagtaattta ttttctgaaa ttatacaatt tgcactaaag
     4921 tagaaattgc tatatttttg atgttcagaa gcttcatctg ataaaacggc ttctccacta
     4981 attaataatg aatctgattc agtattatgt ggggttatac ttttgtaaag aatagaaaat
     5041 tttttacctt taatatgttt ttcaaaatgt tctattactt taggaaaaga tgtaatttca
     5101 gttttgttgt aaaaatcaaa ccattgaatg tcttcagtga cattagctct tagttttttc
     5161 atatcttgca taataaaaaa tccatcaaat atcttctcta ttaaagattt tcctatatca
     5221 gaatatttta taaacatttt ctctgaatta ttctctttag atagtaattt ttcaaattct
     5281 ttgcactcca tgggttttgc ataataataa ccttgggcga tatcacaatt tatactttta
     5341 ataaattcta catgttgttc tgtttcaaca ccttcacata ctgtaagcat cccaagagct
     5401 cttgcaatgt ttattataga gtacaaaatg gttgtatttt taaaatcatt taaattatga
     5461 gataaaaata tcctatcaat tttgattata tcaaaaggta gattttgcaa cataccaaaa
     5521 gatgaatatc cagaaccaaa atcgtccatt gatacagaaa aaccataaga acgaagttca
     5581 gctagttgtt gaataattgt atttgtattt tctattaata tattttctgt tatttcaatg
     5641 ctaaaatttg atgtagagac gctgtacttt tcagtaatac cttttagcat acttggaaaa
     5701 ttagggttaa aaaaatgaag acgagaaaag ttacaacaaa ttggaacaaa aacatcattg
     5761 ttatcaatcc atcttcttat atttttacag ttctcttcaa ataaatgcaa atctaattcc
     5821 gttatcagac caaactgttc aaataaagga ataaatttat ctggagtaag aagtccttta
     5881 atagggtgtc tccatcttgc aagtgcctca catccaacta tagtattatt ttttatatta
     5941 taaagtggtt ggtaaaatac ttcaatttgt ttagtttgta gaacatgtga tatttctgat
     6001 aagaactcct tatcattttc taatttattg taaaaactat tatctttaaa atcaaataga
     6061 atgtatctat ttttgccttc acttttagcc ttttttaaag cactatatac ttggttgaaa
     6121 agtatcaaag tagtcatgtt tggttcacca ttccagatag tgctgccgat actaaatgta
     6181 acattggagt atatttcttt ttcattagga agtcttattt ttaattctcg ggagctctct
     6241 ataatagttg cacccatatt atttaactct ttatttgata catttggaag tacaattaaa
     6301 aaaccttctc cttttaaata agcgactatt ccctttgatt ctggaataaa gtgcaatagg
     6361 ttttgagtta gagtaattaa taaattttct ccgactagat atccatatat atcattaaat
     6421 aatttaaagt tatcgatatt taataacatg attcctatat gtatattttt cgaaatacag
     6481 tcatcaatat attggtacat aaattctctg tttggtattt tagaaaaaaa atctatttca
     6541 tttaaggttt ttaatttaaa atttctacta tctaattcct tagataatac ttctaccttt
     6601 ttatttaaca tttttatata attatgattt tgcatggtat tcctccataa gatactttaa
     6661 attttgatga caactgtatt tatgctagaa gtatttaaat gttaatttgt aaataattta
     6721 aatgctaaat tttaaataag tatttaaaat gaatataca
//
LOCUS       DAMJWP010000192         6750 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.199, whole genome shotgun sequence.
ACCESSION   DAMJWP010000192 DAMJWP010000000
VERSION     DAMJWP010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6750
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.199"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            342..1331
                     /locus_tag="PC700_002989"
     CDS             342..1331
                     /locus_tag="PC700_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDF2582265.1"
                     /translation="MLKEEKTYQEKDAKVFNGVAALIMIVLFLVVSIGIMVFGIVKLD
                     SGFTGIGSLMLILGLVFIVVDFILFFGLKMINPKEAIVLVLFGNYYGTIKKEGYYWVN
                     PFCSAINPAVSRINVSKSSSDEKVDISTNSRGKKVSLKTMTLNNEKQKVNDLLGNPII
                     IGVVVIWKVINATKAVFNVDNYNTFLSIQCDSTIRNVSRLYPYDVSEDGDEKSLRGSS
                     QEIADRLKDELQSRVDIAGIEVCEVRITHLSYAPEIAAAMLQRQQAEAIIAARKKIVE
                     GAVSMVEMALTNLSENNVVTLDEERKAAMVSNLLVVLCGNKDAQPVVNSSSIY"
     gene            complement(1602..2318)
                     /gene="dapD"
                     /locus_tag="PC700_002990"
     CDS             complement(1602..2318)
                     /gene="dapD"
                     /locus_tag="PC700_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HDF2582266.1"
                     /translation="MVNLNDAYEIARYIKEVKKKTPVKVYVNTNSLHFQSTDKFKVFG
                     SNGSYIFVGDYEDIMEVLDKHGNDITDTHVEYDRRNSAIPLKNTLNEHARIEPGAIIR
                     DMVTIEKNAVVMMGAVINIGAVIGEGSMVDMNAVIGARGTLGKNVHLGAGAVVAGVLE
                     PPSATPVIVEDDVLIGANAVILEGVRIGKGAVVAAGAVVTTDVEAGAVVAGSPAKVIK
                     MKDEKTADKTKLMEDLRNLD"
     gene            complement(2542..3291)
                     /locus_tag="PC700_002991"
     CDS             complement(2542..3291)
                     /locus_tag="PC700_002991"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDF2582267.1"
                     /translation="MLKVIATGYDGKMGKILADTIREDNELELVCVAARGLDSYEGDL
                     KIYEDMSTIVEEADVVIDFSHHSNLDNILSYVLKTKTPLVIATTGYNDNEMNKIREAA
                     KVIPVFQSYNMSLGVNVLVKLVKDAAKLLEGFDIEIIEKHHNRKVDAPSGTAVMIANA
                     IKEVLPNVENNYGRYGRSAKRQPNEVGIHAIRGGNIVGEHDALFAGDNEVLTISHQAQ
                     SRGIFASGSIAAAKYLVKQKPGFYNMDSMLG"
     gene            complement(3358..4245)
                     /locus_tag="PC700_002992"
     CDS             complement(3358..4245)
                     /locus_tag="PC700_002992"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDF2582268.1"
                     /translation="MIFKGSAVALVTPFTKDNKVDFDKLGELVEYQIANGTDAIVSCG
                     TTGEANTMTDEEQLATIKYVVEKVNKRVPVIAGSGSNDTMHSVNLSQEAEKLGVDALL
                     IITPYYNKANKAGLKRHFETIANSVKLPIILYNVPGRTCVNISPSLIVELAKIDNIVA
                     VKEASGDLGQVAEIASLVPDDFAIYSGNDDTILPLLSLGGQGVISVLANVCPQETHDL
                     VAKFFEGDIEGSRKLQLGMDALIAALFIEVNPIPVKTAMNLLGFNVGDLRLPLAEMDP
                     ANLEVLKKELVNFGLKVNA"
     gene            complement(4362..5363)
                     /locus_tag="PC700_002993"
     CDS             complement(4362..5363)
                     /locus_tag="PC700_002993"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDF2582269.1"
                     /translation="MKKVNVAIVGATGMVGRTFLKVLEERNFPIENLFLFSSSKSAGS
                     KVMFCGKEYIVEELKETSFTDRGIQIALFSAGGSISEKYAPIAASNGILVVDNSSQWR
                     MNPDVPLVVPEVNPEAIKNHKGIIANPNCSTIQAMVPLKALDDKYKIKRVVYSTYQAV
                     SGSGVKGVEDLEKGMNGNPTGFYPHQISYNCLPHIDSFTENGYTKEEMKMVNETMKIL
                     DNYDLKITATTVRVPVKNGHSESINVEFNNPFELDDLVKTLEEAPGVVVVDNPAKNEY
                     PTAVEFDDRDEVFVGRIRRDFSVDNGVNLWVVADNIRKGAATNAIQIAEMALSYDLV"
     gene            complement(5788..6669)
                     /locus_tag="PC700_002994"
     CDS             complement(5788..6669)
                     /locus_tag="PC700_002994"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDF2582270.1"
                     /translation="MLFKGSAVALVTPFTEDNNVNFEKLGELIEYHIENGTDALVVCG
                     TTGEATTMSESEIFAVIKYTVEKVNKRIPVIAGTGSNNTMLSVHMSQEAEKLGVDGLL
                     IITPYYNKTNEKGLKLHFETIANSVKLPIILYNVPGRTKVNIKPSVVAELAKIDNIVA
                     VKEASGDLAQVAEIAKLVPKDFAIYSGNDDTILPLLSLGGSGVISVLANICPKETHDL
                     VTKFFEGDIEGSKKLQLDMDALIAALFIEVNPVPVKTAMNILGFNVGDLRLPLAEMEE
                     TNLNVLKQELTNFGFKF"
BASE COUNT         2297 a         1113 c          896 g         2444 t
ORIGIN      
        1 tacaatttat cttacattaa aaaaatttaa gtggattttt caaaatatag ataaacttct
       61 ttttcattgt ataaatgata aaaatatatt tgacttcata tcaaaaagag tgccccatga
      121 actaaaaagt taattttgag ataccatatt catatatcat atgatttatt tggaaatcaa
      181 tattaataca ttaaaataac taaatacatg tatgcataaa gatgtataaa aaatatataa
      241 acatatagta ttaattttaa attcaacaat ttaattgtta aaagtgttat tattaaatta
      301 gaaatatctt aacttaatat actgagatgg gagtgataga tatgttaaaa gaagaaaaaa
      361 cttatcaaga gaaagacgca aaagtattca atggagtggc agctttgata atgattgtat
      421 tatttttagt agtttcaata ggtatcatgg tttttgggat agtaaaactt gatagtggat
      481 ttactggaat aggttcactt atgttaattt tgggacttgt atttatagta gtagatttta
      541 tcctattttt tgggctaaaa atgattaatc ctaaagaagc tatagttcta gttttgtttg
      601 gtaactatta tggaactata aaaaaagaag ggtactattg ggtgaatcca ttttgttcag
      661 caataaaccc agcagtatca agaattaatg tatcgaaatc ttcatctgat gaaaaggtag
      721 atatatcaac taattcaagg ggcaaaaaag tatctttaaa aacaatgact cttaataatg
      781 aaaagcagaa agtcaatgat ttattgggaa atccaattat aataggagtg gttgtaattt
      841 ggaaggttat aaatgcaaca aaggcagtat ttaatgtgga taattataat acattcttat
      901 ctattcagtg tgattctact attcgtaatg tttcaagact ttatccatat gatgtatctg
      961 aagatggaga tgaaaaatct cttcgtggta gtagtcagga aatagcagat agattaaaag
     1021 atgaattaca atcaagggtt gacattgcag gaattgaagt gtgtgaagtg agaattactc
     1081 atctttctta tgcaccagag attgcagcag caatgttaca acgtcaacaa gcagaggcta
     1141 taatagcagc acgtaaaaag attgttgagg gggctgtaag tatggtagag atggcattga
     1201 ctaatttaag tgaaaataat gttgtgactt tggacgaaga acgtaaagca gctatggtaa
     1261 gtaatctgtt agttgtgctt tgtggcaata aagatgcaca accagtagta aacagcagtt
     1321 ctatttatta aattataaaa agtttaatag tttattacat tgatatattg aaaaattgaa
     1381 ggaaataaaa atacttgtca tcaagaaata aaaatgatat aagaatgaat aagagaattg
     1441 taaaaaaata aaatgattat aaataaaaga taaaaatatt ataaaaaaga gggtgcctct
     1501 gatgaattaa attcatacag agacaccttt ttaattaatt ttttaaatta gtatattatt
     1561 gtttaattaa tctttctaat taataatata gtactattta attagtctaa atttctaagg
     1621 tcttccataa gcttagtttt gtcagccgtt ttttcatctt tcatttttat aactttagca
     1681 ggagaaccag caacaacggc accagcttct acatcagtag ttacaacagc accagctgca
     1741 acaacagcac ctttaccgat tctaacacct tcaagaataa ctgcattagc tcctattaaa
     1801 acatcatctt caactataac aggagtagca gaaggtggct ctaaaacacc agcaacaaca
     1861 gcaccagcac ctaagtgaac atttttacca agagttcctc tagctccaat aacagcattc
     1921 atatctacca tagaaccttc accaataaca gctcctatat ttataacagc tcccatcatt
     1981 acaacagcat ttttttctat agtaaccatg tctcttatta ttgcacctgg ctcaattctt
     2041 gcatgttcat ttaaagtatt ttttagagga atagcagaat ttcttctatc atattctaca
     2101 tgagtatctg ttatatcatt cccatgttta tctaatactt ccattatatc ctcatagtca
     2161 cctacaaata tataagaacc attacttcca aataccttaa atttatcagt tgattggaag
     2221 tgtaaagagt tagtatttac atatacctta acaggtgttt tctttttaac ttcttttata
     2281 taccttgcta tttcataagc atcgtttaaa tttaccattt gttaattctc cttgtatttc
     2341 attatatttc ttttattata tacttatttg tcaattttag tatatactta cttcttatta
     2401 tttatatttt attttagtta gtttttattg tatagacata gtattaaata aatttattat
     2461 atatgtttga tttgtattgc gtattattat atctatattg catatgttta atttatattg
     2521 tgtattatta tatttatact attatcccaa catgctgtcc atattataaa atcctggttt
     2581 ttgcttaact aagtatttgg cagcagctat agaaccactt gcaaagatac cacgagattg
     2641 agcttgatgg cttattgtaa gaacctcatt gtctccagca aatagagcat catgttcgcc
     2701 aacaatattt ccacctctta tagcatgaat accaacttca tttggttgtc tttttgcact
     2761 acgaccatat ctaccataat tattttcaac gtttggcaaa acctctttta tagcgttagc
     2821 aatcataaca gctgtgccac ttggggcatc tacttttctg ttgtgatgtt tttctattat
     2881 ttctatatca aatccttcta gtaatttagc tgcatcttta accaatttta ctaatacatt
     2941 tacaccaaga gacatattat atgattggaa tacaggaata actttagccg cttcacgtat
     3001 tttgttcatt tcgttatcat tatatccagt agtagcaatt actaaaggtg ttttagtctt
     3061 taaaacgtaa gataagatat tatctaaatt agagtgatga gaaaaatcta taactacatc
     3121 agcttcctca actatagtac tcatatcttc ataaatttta aggtcgcctt catagctatc
     3181 cagacctcta gctgcaacac aaaccaattc taattcatta tcttctctta tagtatcggc
     3241 taaaatctta cccatttttc catcataacc agttgctata acttttaaca taatttcctc
     3301 ctatgattaa gggctaactt ttgaccaaac ttacttttta ccatgcatta atctttacta
     3361 tgcattaact tttagtccaa agttaactaa ttctttcttt aacacttcta aatttgctgg
     3421 gtccatttct gctagaggta atcttaaatc tccaacattg aatcccaata aattcattgc
     3481 agtcttaact ggtataggat ttacttctat aaataaagct gcgattagag catccatacc
     3541 aagttgtaat tttctagaac cttcaatatc gccttcaaag aatttagcta ccaaatcgtg
     3601 agtttcttga gggcaaacat tagcaagtac tgatataact ccttgacctc ctaaagataa
     3661 taaaggaagt atagtatcat cattaccaga gtagatagca aagtcatctg gaactaaact
     3721 agctatttct gcaacttgtc ctaggtctcc actagcttct tttacagcaa ctatgttatc
     3781 aattttagca agttcaacta taaggctagg acttatattt acacaagttc ttccagggac
     3841 attgtaaagt ataattggaa gtttaacact gttagctatt gtttcaaaat gacgcttaag
     3901 tccagcttta ttagctttat tgtaataagg tgttattatt aatagagcat ctactcctaa
     3961 tttttcagct tcttgactta agttaactga gtgcatagta tcattagaac cacttccagc
     4021 tataacagga actcttttat taactttttc aacaacatac ttaatagtag caagttgttc
     4081 ttcatcagtc atagtattag cttcaccagt agtaccacaa gatactatag catcagtacc
     4141 attagctatt tggtattcaa ctaattcacc taatttgtca aaatcaactt tgttatcctt
     4201 tgtaaatggt gtaactagag caacagcaga acctttaaat atcatataaa atcaatcccc
     4261 tcttttttat ttttgtgttt tatattacag ttacttctaa tgcttgtttt tgagttacta
     4321 ttaatatttt acaaaattat atatttatac aagtttgtat attatactaa atcgtatgat
     4381 aaagccattt cagctatttg aattgcgtta gttgcagcac cttttcttat attgtcagca
     4441 acaacccaaa gatttacacc attatcaaca ctaaaatctc ttcttattct tccaacaaat
     4501 acttcatctc tatcatcaaa ttctacagca gttggatatt cgtttttagc aggattatca
     4561 actactacaa cacctggagc ttcttcaagt gtttttacta aatcatctaa ctcaaatgga
     4621 ttgttaaact caacatttat agattcacta tgaccatttt ttactggaac tcttacagta
     4681 gttgcagtta tttttaaatc atagttatca agtattttca tagtttcatt taccattttc
     4741 atttcttctt tagtgtatcc attttcagtg aatgaatcta tatgaggaag acagttgtaa
     4801 gagatttgat gagggtagaa tccagtagga ttaccgttca ttcctttctc taaatcttca
     4861 acacctttaa caccagaacc agatacagct tggtaagtag agtaaacaac tctcttaatt
     4921 ttgtatttat catcaagagc tttaagggga accatagctt gtatagtaga gcagttagga
     4981 ttagctatta tacccttatg attttttatg gcttctggat taacctcagg aactactaaa
     5041 ggcacatctg gattcattct ccattgacta gagttatcta ctactaagat tccatttgaa
     5101 gcagctattg gagcatactt ttcactaata cttccaccag cagagaataa tgctatttgt
     5161 atgcctctat cagtaaatga agtttccttt aattcttcta ctatatattc tttaccacaa
     5221 aacattactt tagaaccagc tgatttagaa gaagagaata aaaatagatt ttctattgga
     5281 aaatttcttt cttctaatac ttttaaaaat gttctaccta ccattccagt agcaccaact
     5341 attgctacat ttactttttt catataaatt caccccaatt ttaaattatt aaaacattat
     5401 tttattttag ttatacaagt aagatatgtt aatattttta tatatccaaa aactttaata
     5461 ttatattaac aactttaatt atatacaaaa aacgtacaaa ggtatacttt gtacgtctaa
     5521 tttatcactc aaagtaaacc ctgtagctca tcacctacaa aaataagtga cagtattaca
     5581 catgttatgt gcaatcccag aaaatattat tgcaagaaat attttcttcg gctgtaaatc
     5641 ctttttccaa tcattaacac ctgccagcta ctattggata ctcttagtta cagctacctc
     5701 taccctaggt taataggata ttaaaatttg attattctaa taataataca tatttaagaa
     5761 aaaatcaact tatttatata aatattgcta aaacttaaat ccaaaatttg tcaattcttg
     5821 ttttaacaca ttaagattag tttcttccat ttcagctaga ggaagtctta aatcaccaac
     5881 attaaaacct aaaatgttca tggcagtttt aacaggaact ggatttactt ctataaataa
     5941 agcagcaatt aaagcatcca tatcaagttg taattttttt gagccttcaa tatcaccttc
     6001 aaagaattta gttactaaat catgtgtttc tttaggacat atgttagcaa gtacagagat
     6061 aacaccactt ccacctaatg ataatagtgg tagtatagta tcatcattac cggagtatat
     6121 agcaaagtct ttaggtacta atttagctat ttctgcaact tgagctaagt ctccacttgc
     6181 ttccttcaca gcaactatat tatctatttt agcaagttcg gccacaacag atggctttat
     6241 attcacttta gttcttcctg gtacgttgta taaaataatt ggcaacttaa cgctatttgc
     6301 tattgtttca aagtgaagtt taagaccttt ctcattagtc ttattatagt aaggagttat
     6361 tattaaaaga ccatccaccc ctaatttttc agcttcttga ctcatatgaa cagacaacat
     6421 agtattgttt gaaccagtac ctgctataac aggtattctc ttattaactt tttcaactgt
     6481 atattttatt acagcaaaaa tttcagattc actcatagta gtcgcctcac ctgttgttcc
     6541 acaaacaact agtgcatctg tgccattctc aatgtgatat tcgattaatt cacctaattt
     6601 ttcaaagttg acattattat cctctgtaaa tggggtaact aaagcaacag cagaaccttt
     6661 aaataacata taatcaaatc tcctttttta tctaaattta attttttaag tacaataata
     6721 attatagtac aaattaatta attttaatag
//
LOCUS       DAMJWP010000193         6702 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.200, whole genome shotgun sequence.
ACCESSION   DAMJWP010000193 DAMJWP010000000
VERSION     DAMJWP010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6702
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.200"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(<1..1667)
                     /locus_tag="PC700_002995"
     CDS             complement(<1..1667)
                     /locus_tag="PC700_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2582271.1"
                     /translation="MKYLIEIAKKERLLVFIYLALGIGIELLNSFSANYYQSLIDRFN
                     NSTINFHIIFIYGTVLVTLCLLRYIDEYPGRRLEHSFFLDLKLKALEKMSKIDYLEYQ
                     MLGTGKLIQKIENGANAGRNIFFNFLLAVVRKIIPSILFSMVFIYRIDKKVMFTILIG
                     YFIVFIITNLLIKALYQIKEHILINEEKMNHFLVRGFVEMVVFRVNKRFEYEICQSKF
                     AKKEIIDSRVKMTLIHEAFFTIFSLIVTFIKMLIIVYSWKTKTISIGSIIALITLLDN
                     AYTPIAIFNVLYIQFKLDKAAYKRYTDILELNNDGQLLAGKEVNLMYGNIEFNEISFL
                     YNNKREIFNKLSLSIKSGEKVAIVGESGSGKSTLIKLLIGLLKPSEGTIFIDDYNLLE
                     MNLNSYYNYVTYVSQDSPIFDGTLRENIVFGKTIKSNDIIEVLEKVGLKNLYLKLEAG
                     LDTKLGEKGTTLSGGEKQRLALARLWFSDAKIIILDEATSALDNITEEKVMLNVMEFL
                     KDKTTIMIAHRFNSIKNVENIIVLKEGSIIGSGSFKKLLDGNLYFRE"
     gene            complement(2590..3171)
                     /locus_tag="PC700_002996"
     CDS             complement(2590..3171)
                     /locus_tag="PC700_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2582272.1"
                     /translation="MNNKKHWYDYLWIFSSIYLVLGFVNIIFAWIGLICFFVPLAISI
                     VKGNKAYCNKYCGRGQLFNLLGNKLKLSRNRDIPKFIRSKWFRYGFLTFFMVMFANML
                     FSTYLVFEGSRNFKEVVTLLWTIKMPWQWAYHGTLVSPWVVQFAFGFYSVMLTSTVLG
                     LITMILFKPRSWCVYCPMGTMTQMICKAKSKSV"
     gene            complement(3164..3394)
                     /locus_tag="PC700_002997"
     CDS             complement(3164..3394)
                     /locus_tag="PC700_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2582273.1"
                     /translation="MSNKKKKYANILANECVACGSCIKACPRSAISVPCGISAKINRD
                     LCVGCGICEKICPASVIEIITILKEEEGKCHE"
     gene            complement(4719..4994)
                     /locus_tag="PC700_002998"
     CDS             complement(4719..4994)
                     /locus_tag="PC700_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2582274.1"
                     /translation="MLELTKIIELLEEHKIEVSIPKELHKYSLLEYLELCQQSGGTYF
                     YIKTLEGALKEARNELMIKLFNGKNYMELARMFEMSVINVRHIVRKK"
     gene            complement(5070..6371)
                     /locus_tag="PC700_002999"
     CDS             complement(5070..6371)
                     /locus_tag="PC700_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2582275.1"
                     /translation="MGKSKDVEIEKSILGTILLDNKLSYKLDELNENMFMNDICLEIF
                     KIMKELKKENIVIDVATVKSKIDRKSLAIKTSDVTNLITWGQNFGLDGHIKILKENLA
                     RRSINQNCQNLLHSLNLGENIDTCIYKFESNIKEILDKDTYENDDVNSIAGKVLDFLE
                     NKKDIGFKFGIKLLDTTIGGLFKGELTTIAAKSGVGKTALALQIMLNSFKQGKKTLFI
                     SREMTSEQVFMRNICRVTGVSTRNMKSKEIDENDWKLIVNAIGDLSENNLIYINDKID
                     TISAIRKRIRQVKPDLLIVDYVQLLTSQKSMDKREREVATFSRELKNMTLDFNIPVIQ
                     LSQLNDEMKDSRPYGDRPMRDSKAIYHDSNNVVYIHQLKGSDYEEAVRDIGESEEAVR
                     ASEYRGIKMVDLIVAKCRDGQTRHKHFCYFGDKLHFQELNY"
BASE COUNT         2298 a         1231 c          546 g         2627 t
ORIGIN      
        1 aattctctaa aatataaatt accatctaat agttttttaa agctaccact acctattata
       61 ctcccctctt taagaacaat tatattttca acattcttaa ttgagttaaa tctgtgagca
      121 atcataatag ttgttttatc tttcaaaaat tccataacat ttagcataac tttttcctct
      181 gtaatattgt ctaaagctga tgtagcttca tctaatataa ttatcttagc atctgaaaac
      241 caaagccttg ctaatgctag cctctgcttt tctccacctg ataatgtagt tcctttttca
      301 cctaatttag tatctaaacc agcttctaat tttaagtata aattttttaa acctactttt
      361 tctaagactt caataatatc attacttttt atagtttttc caaatacaat attctcacgt
      421 aaagttccat caaatatagg tgaatcctgt gacacatatg taacataatt atagtaacta
      481 tttagattca tctcaagtag attataatca tctataaata tagttccttc gctgggcttt
      541 aataatccaa ttaatagttt aattaaagtt gatttaccag aaccactctc tccaacaata
      601 gctacttttt ctccactttt aatacttaaa ctaagtttat taaatatctc tcgcttatta
      661 ttatacaaga agctaatctc attaaattca atatttccat acattaaatt aacttcttta
      721 cctgctaaca gttgaccatc attattaagt tccaaaatat ctgtatagcg tttatatgct
      781 gctttatcta gtttaaattg aatatacaat acattaaaaa tagctatagg agtatatgca
      841 ttatctagta aagttatcaa tgcaattata gaacctatac ttattgtttt agttttccaa
      901 ctataaacta ttattaacat ttttatgaac gtcactatta atgaaaaaat cgtgaagaaa
      961 gcttcatgaa tcagtgtcat tttaacccta gaatcaataa tttctttttt cgcaaatttt
     1021 gactgacaaa tttcatactc aaatcttttg tttacccgaa atacaaccat ttctacaaat
     1081 ccacgaacta aaaaatgatt catcttctct tcattaatta atatatgctc tttgatttga
     1141 tatagagctt ttattagtaa attagtaata ataaaaacta taaaatatcc aattaatatg
     1201 gtaaacataa cttttttatc aattctataa ataaaaacca tactaaataa aattgatgga
     1261 attatttttc taacaacggc taataaaaaa ttaaaaaata tatttctgcc agcattagct
     1321 ccattttcaa ttttttgaat taattttcca gtgcctaaca tctgatattc taagtaatct
     1381 attttactca ttttttctaa agcctttaat ttcaaatcta aaaaaaaact atgttctagt
     1441 cttctacctg gatattcatc aatatatctt aaaagacata aagtaactaa tacagttcca
     1501 tagataaaaa ttatgtgaaa atttatagta ctgttattaa atctatctat taggctctga
     1561 taataattag cactaaaact atttaaaagt tctattccta tacctagtgc taaatatata
     1621 aaaactaata acctctcttt ttttgctatt tctattaaat atttcataac tgtacaccta
     1681 tccttattta ttttacttta ttaacttaaa ataattttat agataaaatg tttctttatt
     1741 tgtttaaatt tagtgtgatt attaatcatt tcaagttaac aaaacctaaa gtttttagca
     1801 gtacagttct atttgttagc aaattattat tttactcagt acattatgct aacatataat
     1861 ctaactgtac tgagtaaata tctcgtttca tcatataaaa tctccttttt atagtttaat
     1921 atatatagtt aaatttacta tataatctga atatttattt ttctatcacc tttaccagaa
     1981 ataaaatcaa ttatttattg tataagcaaa acttcataat attttcaaca tataattatt
     2041 atacagaact cctaagttaa tttaaagtta gaatttattt ttataagaaa tgtcattgac
     2101 ctacctttaa taaaacagcc accatttttt tatgtattaa catataatat atagtaatga
     2161 aaacatatta attaaaaaat attgtataaa ttagtttatc tgtaacaaat ccagtttatg
     2221 tttcaacaag cacgtaagac ttcttgaaat ttaatctgct tcctttaaat atctattact
     2281 tgttttaagc ataagtttac aatcttctat aacatttatc atttcttcag atttctctga
     2341 cgtacttaaa atacaagttg ttacattgtt tttctattta attagtaatg tatcatgtaa
     2401 aaaagaagca taaatgagtt tactttatac aaacttcatt tatgctccac tcatatacct
     2461 atcatagcta atagaaacta ttactcatct tgaatctatt tcatatttag atatttatat
     2521 ctttcttatt ttcttaaatc aactctagct ttaaacatta aaccccttaa atcttcacaa
     2581 aatctcttcc tatacagact tactctttgc cttacatatc atctgagtca tagtacccat
     2641 agggcaataa acacaccatg aacgaggttt aaacaatatc atagtaatca aaccaagaac
     2701 agttgatgta agcataacac tataaaaacc aaatgcaaac tgtaccaccc aaggtgaaac
     2761 taaagttcca tgatacgccc attgccaagg catttttata gtccacaaca aagtcactac
     2821 ttctttaaaa tttctacttc cttcaaacac taaataagtt gaaaaaagca tattggcaaa
     2881 cattaccata aaaaatgtca aaaacccata tctaaaccac ttacttctaa taaatttagg
     2941 aatatctcta tttcttgaca gcttcaattt atttccaagc aaattaaaca gttgccctct
     3001 tccacaatac ttattacagt aagctttatt tcccttaaca atagaaatag ctagtggaac
     3061 aaagaaacaa attagcccaa tccaagcgaa aataatattt acaaatccca ataccaaata
     3121 tattgaagaa aaaatccata aatagtcata ccaatgtttt ttattattca tgacacttac
     3181 cttcttcctc ttttaatatt gttataatct caattacaga tgctggacat atcttttcac
     3241 aaatcccaca tccaacacat aaatctctat taatttttgc actaatgcca catggaactg
     3301 agatagcact tcttggacat gcttttatac agctaccaca agcaacgcac tcatttgcta
     3361 aaatatttgc atacttcttt tttttattag acataataac ctccctatat tattaatcta
     3421 taagaattat atcaagatac ttacttttta tctgtgactt aatcacataa aacctcaata
     3481 ttaaaaatca cactctaaat acaaaggagt ttcaaataaa aaacgatatg aagtcattaa
     3541 gtttagcttc atatcgcctt ttcttttgtg tatctatata aatatttatt atctaatatt
     3601 taatatatct ataatatttt cttatacagc tctataattt tacacctaat catattctat
     3661 tagtaacagg ttaataccca tctaaaacta taactttatt cacaatctta ttctattagc
     3721 cacaagttaa actcatattg aagtttatct ttagttacac tcttactcta ttaatcacaa
     3781 gttaagccca tattgaagtt tatctctatt tacactctta ctctattaat cacaagttaa
     3841 gctcatattg aagtttatct ctatttacac tcttactcta ttaaccacaa gttaaactca
     3901 tattgaagtt tatctctatt tacactctta ctctattaat cacaagttaa gctcatattg
     3961 aagtttatct ctatttacat tcttactcta ttaaccacaa gttaaaccca tattgaagtt
     4021 tatctctatt tacactctta ctctattaac cacaagttaa gcccatattg aagtttatct
     4081 ctatttacat tcttactcta tcaatcatag gttaagctca tattgaagtt tatctttagt
     4141 tgcaccttac tctattaatc acaagttaaa cccatattga agtttatctt tagttacacc
     4201 ttactctatt aaccacaagt taaacccaca ttgaagttta tctctattta cactcttact
     4261 ctattaatca caggttaaac ctattataat cttaacttta ttcacaattt tattctatta
     4321 gccacaggtt aaatctatta taatcttaac tttattcaca tattacatca ttaatcgcat
     4381 atcaagattt tataataagc tttaactaaa cacacgattt taccttattc gtatagttta
     4441 atctattaaa ttattatata atcttaagca aacatacaac tctacactat taattatagc
     4501 ccctcctttt aaatactcat aatcttatac acaaccttac cttattaatc acacatttaa
     4561 ctcatatata tcatcaattt tattcacaaa atcatgctgt taatcacact tatactcctc
     4621 tataatctta aacttataca caaattagcc acaattttat ttcaactata ataacttcta
     4681 tttcagaagt atatcacact tgtattctcc aaaaatatct atttttttct aacaatatgc
     4741 ctgacattaa taacactcat ttcaaacatc ctcgctaatt ccatataatt tttcccatta
     4801 aacaatttaa tcataagctc atttctagcc tcttttaatg ccccttccag tgttttaata
     4861 taaaaatatg ttcccccact ttgttgacac aattcaaggt attccaataa actatactta
     4921 tgtaactcct ttggaatact tacttctatc ttgtgttctt caagtaattc tataattttc
     4981 gttaattcca acatattttc acctcatatt ttataatcta tttgctattt cctgactttt
     5041 aataagcttg ttattactac tttattgacc taataattta gttcttgaaa atgtaactta
     5101 tccccaaagt aacaaaaatg cttatgccta gtctgtccat ctctacactt cgcaactatt
     5161 aaatcaacca tcttaattcc cctgtattct gatgctctaa ctgcctcctc actttctcca
     5221 atatctctca ctgcttcttc ataatcactc ccctttaatt gatgtatata aaccacattg
     5281 ttgctatcgt ggtagatggc tttgctatcc ctcataggtc tatctccata aggtcttgaa
     5341 tctttcattt catcatttaa ctgacttaat tggattacag gtatattaaa atctaaagtc
     5401 atatttttaa gttctctcga aaaagtagct acctcccttt ctctcttatc catgcttttc
     5461 tgagatgtaa ggagttgcac ataatccact attaataaat ctggctttac ctgccttatc
     5521 ctctttctaa ttgcactaat tgtatctatc ttatcattaa tataaattaa gttattttcg
     5581 cttaaatcac ctatagcatt tacaataagt ttccaatcat tttcatctat ttctttagat
     5641 ttcatattcc ttgtacttac accagttact ctgcatatat ttctcataaa tacttgctca
     5701 ctggtcattt ctctacttat aaataaagtt tttttacctt gcttaaatga atttaacatt
     5761 atttgaagtg ccaaagcagt tttcccaacc cccgatttag ctgctattgt ggttagctct
     5821 cctttaaaaa gacctcctat agtagtatca agtaatttta taccaaattt aaatccaata
     5881 tcttttttgt tttctaaaaa atcaagcacc ttccctgcta tactatttac atcatcattt
     5941 tcataagtat ccttgtccaa aatctccttt atattacttt caaatttgta aatacaagta
     6001 tcgatatttt ctccaaggtt taggctgtgt aaaaggtttt ggcagttttg atttatgctt
     6061 cttctagcta aattttcttt taaaatttta atatgaccat caagaccaaa attctgaccc
     6121 caagtaatta gattagtcac atcactcgtt tttattgcta gagattttct atctatcttt
     6181 gatttcacag tggccacatc aatcactatg ttttcttttt taagttcttt cataatttta
     6241 aatatctcaa gacatatatc attcataaac atgttttcat ttaattcatc tagcttatag
     6301 cttaatttat tgtctaaaag aattgtgcct aaaatagact tctctatttc aacatctttt
     6361 gatttaccca tataaattaa tctctctttc cttataagtt tttatatttt gatttttaaa
     6421 tacattatca attataaata cattatttgt tatgactcac tatttgttat gactatatta
     6481 ttcgtcatga tatactacaa attattaata tactatttat tctgatacat ataatttact
     6541 acataatagc aatatcttat ttgatataac ttactaccaa ttattaatac attgttcaat
     6601 ataattcatt actcgatagt aatatcttat ttgatataac ttactgccaa ttattgatat
     6661 atcgttcaat ataattcact acctgatagt aatatcttac tt
//
LOCUS       DAMJWP010000194         6625 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.201, whole genome shotgun sequence.
ACCESSION   DAMJWP010000194 DAMJWP010000000
VERSION     DAMJWP010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6625
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.201"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..957
                     /locus_tag="PC700_003000"
     CDS             <1..957
                     /locus_tag="PC700_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2582276.1"
                     /translation="SKVNMFFDGSKFVGGKSIVFSIIGIIFLIILISGIAYGYWVTKC
                     ISNITSAVSDISKRSYLPVNIKGVFADIYNSLNILNEEIHRSDEIQNKTDIMREEWIS
                     NITHDLKTPLSPIKGYAELLTDSSTNYSPEKVYKYASIMLKNIEYTNTLINDLKLTYQ
                     LKNGIIPLNKNKYDLVRFLKELSIDILNYPEYESNIVEFKSNIDNINFDFDCTLLKRA
                     FSNLIVNAFTYGDKNTKVCVHITLNERIEIIISDNGKGMSREEASNLFNRYYRGSNTK
                     QKIEGTGLGLAITKQIIEVHGGNISVESIPGLGSSFYITFPI"
     gene            1177..3654
                     /locus_tag="PC700_003001"
     CDS             1177..3654
                     /locus_tag="PC700_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2582277.1"
                     /translation="MKIILKYIFTNIKERKIRTAVMLLSIVLSTVLLFVSFSIGLSYE
                     SAQRKMAKGMYGTATISVQSKNPDVLTNLEDIPDLNAIKSKVGVLESSAIYNKGGYYE
                     EFSLISADLSQLNKINKPRLENGDSITDFSGDKIILPNRFTSKYKIKKGDSITLQIYG
                     KSYTFQVSDIASYDTVFLRNTRGVNALLPKETLSKIINKGSGYTRVLIESEEEMTENL
                     VNKLSEELSTEKYMVSNTINETKIISDARQKTMPFFLISFFALTLSIFIIYSSYKVIT
                     LERLPFIGTFRSIGANEKTVTRILMLESILYGSIGGLIAIPIGVVVLNLMLHGLGNSL
                     EQGISIPVVISPIGVIISVIVAIIVSSFSAYIPVKKASHLPIKNIVLGTVEEKNVSNR
                     SILFIGSIMFILSILLPRISPENTLYLAGGFSLLGLIVATIVLIPLITDIMSIVFEFV
                     YKNILGNEGKLAARNMKNNKNIIQNITLLFISISAVIAISVVGNFVKTYITDVFRDAE
                     LQGFADGKMNEEFIENVRHMDGIKKILPLYVMNNEISGNGVTLSRLEGTDNIKLYNSM
                     FGINYTNFEIKKQVIEAFNDKRSVILNEDTLKKVGLSIGDTITLSNDKYDFSYKIVGS
                     FKSRANDVEAVIPSHYAVNDFDKTNYGFLVYTAVNPDVVMIQIRDLFGDTYNWSRTVE
                     EFNNDSLNTISSFLSPMNKMTYFIFLLATVGIINNLLINYIQKKRSIAMYKSIGLSDK
                     QNIKVTLIEGFTSGLLGAVIGIVISILEIQTIFIVAGPKISMKPDLDFKTFIIVGLLG
                     IIVTLIGSIVPIIKGKKMKLIEEIKFE"
     gene            3669..4355
                     /locus_tag="PC700_003002"
     CDS             3669..4355
                     /locus_tag="PC700_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2582278.1"
                     /translation="MKITHNSLAVEAKNIIKEYKIGNTTTRVLKEVSLQVMKGEFVSI
                     MGQSGSGKSTLLYILGGLDTPTSGKVYMNGADISHFNDEKMSIIRRRNIGFVFQFYNL
                     IPNLNVEENIMLPLLLDGKNLKDYKNQLDEILDIVGLTDRRKHTPRELSGGQQQRVAI
                     ARALVGKPEILFADEPTGNLDSKTGIEIIDLLNKINRDNGQTIIMVTHSPEAAKSSSR
                     TITVSDGLIV"
     gene            4628..5461
                     /locus_tag="PC700_003003"
     CDS             4628..5461
                     /locus_tag="PC700_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2582279.1"
                     /translation="MLKKESIHFINKNRSLIVCEDNFVYLLPCMQLKNWISNFTISFP
                     NQTIISDNYTIIPHGSVTLVFFYDATGLHSLLFGPTTKPKTVGNIANRCDVILIIEFQ
                     PAGFFPLIGIQQSELIDKVVPFSIINTSLDLEIKKIFNESLSIDKLILKLEELLISNI
                     KIEYSYEFILAIQLIIQNSGNISSQEISKKVFYSSRHLNRLFNQYLGLSMKSFSRLVR
                     INKSIKLLNNNKTSLMYICNELGFYDIPHFIKDFKIVCGITPQKYRANMSDFYSEIAK
                     F"
     gene            5499..5960
                     /locus_tag="PC700_003004"
     CDS             5499..5960
                     /locus_tag="PC700_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HDF2582280.1"
                     /translation="MKFGGVLLIVKDINKSKKFYEDVMEQKITLDLGEHVSFENGFGL
                     QSNYKAIIGYDLPIYQQSNNFQLYFEVNDIKQWESKINRIGNIEFLHYIKEYPWGQRT
                     FRFYDFDKNIIEISESMESVIKRLLKQGLALEEISKRTMYPVEFITQFQQL"
     gene            6113..6538
                     /locus_tag="PC700_003005"
     CDS             6113..6538
                     /locus_tag="PC700_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2582281.1"
                     /translation="MHDELKIKMYSFTMDCKDPHELAKFYAALLNWEIAFYNEEYACV
                     GAPGTNQGTYPGITFQRNSEYKPPVWPEKPESQQQMAHMDFAVNDLEEAVQYEIHCGA
                     TIAEEQFTDDWRVMIDPAGHPFCLCQMKSIMESSHFSLL"
BASE COUNT         2542 a          726 c         1059 g         2298 t
ORIGIN      
        1 tctaaagtga atatgttttt tgatggaagt aaatttgtag gtggtaagtc aattgtattt
       61 tcaatcatag ggattatatt tttaataatt ttgatttctg gaattgctta tggttactgg
      121 gtaacaaagt gcatatcaaa tattacatct gctgttagtg atatttcaaa acgaagctat
      181 cttccagtta atattaaagg tgtttttgct gatatatata atagtttaaa tattttaaat
      241 gaagaaattc atagaagtga tgagatacaa aataaaactg atattatgag agaagagtgg
      301 atttcaaata taactcatga tttaaaaact ccactttcac caataaaagg gtatgcagag
      361 cttcttacag atagtagtac taattattca cctgaaaaag tatataaata tgcttcaatc
      421 atgttaaaga atatagaata tacaaataca cttataaacg atttaaaatt aacctatcaa
      481 ttaaaaaatg gaataattcc tttaaataaa aataaatatg atttagtaag attcttgaaa
      541 gaattatcca ttgatatttt aaattatcca gaatatgaat caaatatagt tgaatttaag
      601 agtaatatag ataatattaa ctttgatttt gattgtacac tattaaaacg agcttttagt
      661 aatcttattg tcaatgcttt tacatatgga gataaaaata ctaaagtttg tgtacatata
      721 acattaaatg aaagaattga aatcattata tctgataatg gaaaagggat gtcaagagaa
      781 gaagcatcaa atttgtttaa tcgttattat agaggaagca atacaaaaca aaaaatagaa
      841 ggaactggtt taggactagc aataacgaag caaattattg aagttcatgg agggaatata
      901 tctgttgaaa gtatacctgg attaggtagc tcattttata tcacctttcc tatttaatta
      961 aggttaatta aggtcaaata aaaaatatat taaggttgat agattattat atttatatag
     1021 tagtttatca atctttttta tttaaggagg tgatttgatt aaaaatatta taaatagaat
     1081 gataaaagta tggaggagtc tattagagat tcaatttgga ttaaagtata aaaatattat
     1141 actgaaatat cttagataaa ggaagaaggt gtaaatttga aaattatatt aaagtatatt
     1201 ttcacaaata ttaaagaacg aaaaattaga acagcagtta tgttactgtc catagtttta
     1261 tcaacagttt tactttttgt ttcattctca attggattat cttatgaaag tgcacaaaga
     1321 aaaatggcaa aaggtatgta tggtacagcg actatatctg tccaaagtaa aaatccagat
     1381 gtattaacca atttagaaga catacctgat ttaaatgcta taaaatcaaa ggttggtgta
     1441 ttggaaagct ctgctattta taataaaggg ggatactatg aagaatttag tcttatatca
     1501 gcagacttaa gtcaactaaa taaaatcaac aagccacgat tagaaaatgg cgatagtatt
     1561 actgattttt ctggtgataa aattattctt ccaaatcgct ttacatcaaa atataaaata
     1621 aaaaaaggtg attctattac tttacagatt tatggaaagt cttatacttt tcaagttagt
     1681 gatattgcat cttatgatac agtatttttg aggaatacaa gaggtgtaaa tgcactgcta
     1741 cccaaagaga ctctatcaaa aattataaat aaaggcagtg gatatacaag agttcttata
     1801 gagtcagaag aagaaatgac agaaaatcta gtaaataaat tatccgaaga actttccact
     1861 gaaaaatata tggtatcaaa tactataaat gaaactaaga taatttcaga tgctagacaa
     1921 aagacaatgc ctttcttttt aataagtttt tttgcattaa cccttagtat atttatcata
     1981 tacagcagct ataaggttat tacattagaa cgtcttcctt ttattggaac atttagaagt
     2041 attggtgcca atgaaaaaac tgtaactcgt atattgatgc ttgaaagtat attatacgga
     2101 agtataggag gattaattgc tattcctatt ggtgtagttg ttttaaattt aatgcttcat
     2161 gggttaggaa attctctcga acaaggaata tcaattccag tagtaatatc acccattgga
     2221 gtgattatat ctgttatagt agcaataatc gtttcttcat ttagtgcata tatacctgtt
     2281 aaaaaagcta gccatttgcc aataaaaaat attgtactag gaacagtaga agaaaagaat
     2341 gtttcaaatc gttctatact ttttatagga agtataatgt ttattttatc tattttactt
     2401 ccaagaatat cccctgaaaa tacattgtat ctagctggtg gattttcatt actaggatta
     2461 attgtagcta ctattgtgtt gattcctctt attactgata tcatgtctat agtttttgag
     2521 tttgtttata aaaatatttt agggaatgag ggtaaattag ctgctagaaa tatgaaaaat
     2581 aacaaaaata taatacagaa tatcacttta ctatttataa gtatttctgc agttattgca
     2641 ataagtgttg ttggtaactt tgtcaaaact tatataacag atgtgtttcg tgacgcagaa
     2701 ttacaaggtt ttgcagatgg aaagatgaat gaagagttta ttgaaaatgt tagacatatg
     2761 gatggaatta aaaaaatatt acctctttat gttatgaata atgagatttc tggtaatggt
     2821 gtaacattat caagactaga gggtacagat aatataaaac tatataactc tatgtttgga
     2881 atcaattata caaattttga gataaaaaaa caagtaatag aagcttttaa tgataaacgt
     2941 tctgttattt tgaatgaaga tactctaaaa aaagtaggct tatctatagg agatacaata
     3001 actctatcta atgataagta tgatttttct tataaaatag taggtagttt taaatcccgt
     3061 gcaaatgatg tagaagctgt tataccatca cattatgcag ttaatgattt tgataaaact
     3121 aattatggtt ttttagtata tacagcagtc aatccagatg ttgttatgat tcagataaga
     3181 gatttatttg gagatacata taattggagt cgtacagttg aagaatttaa taatgattct
     3241 ttaaatacta taagtagttt tttgtctcct atgaataaga tgacctattt catttttctt
     3301 cttgctacag taggtattat taataatcta ctgattaatt atattcaaaa aaaacgttct
     3361 atagctatgt ataaatctat aggactaagt gataaacaaa atataaaagt gacattgatt
     3421 gaagggttta cttcaggttt acttggagct gttattggta ttgtgatatc tatacttgag
     3481 attcaaacta tctttatagt tgcaggacca aaaatttcaa tgaaaccaga tttagatttt
     3541 aaaacattta ttattgttgg gttattggga attatagtaa ctttaatagg ttcgatagtg
     3601 cctattataa aagggaagaa aatgaaactg atagaagaaa ttaagtttga ataattatgg
     3661 aggttaatat gaaaattaca cataatagtt tagctgtaga agcaaaaaat attatcaaag
     3721 aatataaaat tggaaataca acaactcgag tgttaaaaga ggtatcttta caagtaatga
     3781 aaggtgaatt tgtttctatc atgggtcaat ctggttctgg taaaagtact cttttatata
     3841 ttttaggtgg tttagatact ccaacgagtg gaaaagtgta tatgaatgga gctgatatat
     3901 cacattttaa tgatgagaaa atgagcataa tacgtcgtag aaatatagga ttcgtatttc
     3961 aattttataa cttaatacca aatttaaatg tggaggaaaa tataatgctt cctttgttat
     4021 tagatggtaa aaatttaaaa gattataaaa atcaattaga tgaaatctta gatatagttg
     4081 gtttaactga tagacgtaaa catacaccac gagagttatc tggtggtcag caacaacgtg
     4141 ttgctatagc tcgagcttta gttggaaaac cagagatttt atttgctgat gagcctactg
     4201 gaaatttaga tagtaaaaca gggatagaaa taatagattt actgaataaa ataaacagag
     4261 acaatggaca aactataatt atggtaactc actcaccaga agcagccaaa agtagtagtc
     4321 gtactattac agtttcagat ggtttaattg tataaggact aattattgta tttgagataa
     4381 gcatttttaa ctatataaat tagacatatg taaagtggat ataagttaaa tactataaat
     4441 gtgctataat attaaaataa cagataaaag tactatattt tttattgatt attttaattt
     4501 atttgtttat taaatatcta atgagtattc atactatctc tattattaaa agcagttaat
     4561 gatatataga gataatatgg attcttctta ctttaataaa tattcacgat aggacaggat
     4621 ataatatatg ttaaaaaaag aaagtattca ttttataaat aaaaataggt cacttatagt
     4681 ttgtgaagac aattttgttt atctcttgcc ttgtatgcaa ttaaaaaatt ggatatcgaa
     4741 ttttactata tcgtttccaa atcaaacaat aatttctgat aattacacga ttattcctca
     4801 tggaagtgta actttggtgt ttttttatga tgccacaggg ttacatagtc tattatttgg
     4861 tccaactact aaaccaaaaa cagtaggaaa tattgctaat agatgtgatg ttatactaat
     4921 tatagaattt caacctgctg gttttttccc gcttataggt atacagcaaa gtgaacttat
     4981 tgataaagtt gttccatttt ctataattaa tacttcatta gatttagaaa ttaaaaaaat
     5041 atttaatgaa tctttatcta tagataaatt gattttaaag ttggaagaat tattgatttc
     5101 taatattaaa attgaatact catatgagtt tattttggca attcagttga ttattcaaaa
     5161 ctctggaaac atatcttcac aagaaatttc taaaaaagta ttttatagtt ctaggcattt
     5221 aaacagactc tttaatcagt acctagggtt gagtatgaag tcattttctc gtttggtacg
     5281 tattaataaa tctataaaac ttctaaacaa taataaaact tcattgatgt atatatgtaa
     5341 tgaattagga ttctatgata taccacattt tattaaagat tttaaaattg tctgtggaat
     5401 aactcctcaa aaataccgag ctaatatgtc tgatttttac agtgaaattg caaaatttta
     5461 agttataata cttataacaa agaaaggata gtgataagat gaaatttgga ggagtattgc
     5521 taattgtcaa agacataaat aaatcaaaaa agttttatga agatgttatg gagcaaaaaa
     5581 ttacattaga tttaggagaa catgtatcat ttgaaaatgg atttggatta caaagtaatt
     5641 ataaagctat aattggatat gatttaccaa tatatcaaca atcaaataac tttcagttgt
     5701 attttgaggt taatgatatc aaacaatggg aaagtaagat aaatagaata ggaaatattg
     5761 aatttttgca ttatataaaa gaatacccat ggggtcaaag aacttttagg ttttatgatt
     5821 ttgataaaaa tattatagaa atttcagaaa gtatggaaag cgtcataaag aggcttttaa
     5881 aacaaggttt agctttagaa gaaatttcaa aacgcactat gtatccagtt gaatttatta
     5941 cacaatttca acaattataa aatattaaaa tatataattt tttctttata ttgcaaaaaa
     6001 caaaggcttg gggcataata gaaaaatact tgaaataaat tacatactta atgtttgaat
     6061 ttagcttaaa aatgaaaaga cgaagaaatt tatacttgga ggtaattacg ttatgcatga
     6121 tgaactgaaa atcaaaatgt actcatttac gatggattgc aaagacccac atgaattagc
     6181 aaaattttat gcagcgttac tcaattggga aatagcattt tataatgaag aatatgcatg
     6241 tgttggtgct ccaggaacaa atcagggaac atatcctggt ataacatttc aacgaaattc
     6301 tgagtataaa ccgcctgtgt ggccagaaaa acctgaatct caacagcaaa tggctcatat
     6361 ggatttcgct gttaatgatt tagaagaagc agttcaatat gaaattcatt gtggagctac
     6421 aatcgcagag gagcaattta ctgatgactg gagagttatg attgacccag ctggacaccc
     6481 tttttgctta tgtcagatga aatcaattat ggaaagtagc catttttcat tgttatagac
     6541 caaaatattg tttattaatt acaaaaaagc acaacagtat ataccgttgt gcttttactt
     6601 attatacatg aatacactaa aaaat
//
LOCUS       DAMJWP010000195         6574 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.202, whole genome shotgun sequence.
ACCESSION   DAMJWP010000195 DAMJWP010000000
VERSION     DAMJWP010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6574
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.202"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(502..1170)
                     /locus_tag="PC700_003006"
     CDS             complement(502..1170)
                     /locus_tag="PC700_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HDF2582282.1"
                     /translation="MPVLMAIGVPVHLAIGTNKFAASAGCISSAYRYAKSGKINNDLL
                     KKLVPFTIIGSVLGVRCVLSISEEILNVLVVVMILIVAIYTFISKNLGQEDNFEAVNK
                     KNLRLGMLMALIMGFYDGFFGPGTGTFLTFGFIKIYGYDFLHASANTKILNLTSNITS
                     LLLFMINGQVDYKIAIVFALVMIMGAYVGAKVAIKKGSKMIKPIFLVMALFMVVKLVY
                     QTLV"
     gene            complement(1115..1255)
                     /locus_tag="PC700_003007"
     CDS             complement(1115..1255)
                     /locus_tag="PC700_003007"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582283.1"
                     /translation="MFSVIFLCIGGFLAAFVDSIAGGGGTYKHACSYGNWSASTSCHW
                     YK"
     gene            complement(1314..2969)
                     /locus_tag="PC700_003008"
     CDS             complement(1314..2969)
                     /locus_tag="PC700_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2582284.1"
                     /translation="MVDFIESNNEVQKFDWGEVMWIHEPSKTQFNRLSAGIVRFFPGN
                     CQEKHFHLSEEQLLYVIQGEGIQMIDGKKVNIKETSIVYCPPYSEHEIINTGKIDLVI
                     LITYVPHKFSSLRQIPIVFSENNIQELVNVNIIENLANQISNILKLRISVYDLKYKGI
                     FETKKENKFCDICKNIKPCTKKLVKSINNDNFEKVYHCEYGVSSLEIPIVLEENIVGY
                     IECGNFIVYKSIDIDKNLSELSSSSGIDIKYINKIYNEFPLNPKSRLYVLKENLLMMS
                     EFIQEIAKRNFFENQLSIKDEEILKSRKENIRLEEALKKANSRLLEEEYIINPIDMFN
                     NSSKEYPFELELYIEKEIKNMNLEGVYNLIDTNKLRYNDVRDTIQEMIFVLSRTVLRD
                     LEDLKLISYLRNKYNKKISLVDSDEDLWNVLFEFSKECIDKNRAFWRKDKGNLIENIN
                     EYIKKYYKENINLNSISDVFFISPNYLSSIFNERNKVSITEYINLLRIEESKKYLLDR
                     SMSISDICKKVGFNNSSYFSQIFKKFNSITPNEYRKNMLDNKY"
     gene            complement(3198..3566)
                     /locus_tag="PC700_003009"
     CDS             complement(3198..3566)
                     /locus_tag="PC700_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2582285.1"
                     /translation="MKYSITDVSKITKISSSAIRFYEEKGLLLPINRKPSGVRYFSEE
                     DVERLIFIKELKNTGLSIKDIHECFHFCDMGDGTIDKRIQLFQQHKQNIKNKIHELES
                     CMDKIDEKIYYLKEKKMCKK"
     gene            3715..4509
                     /locus_tag="PC700_003010"
     CDS             3715..4509
                     /locus_tag="PC700_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase"
                     /protein_id="HDF2582286.1"
                     /translation="MEKYATMIKEKLNEGKVLCGVAVSLSDSVVSELIGISGYDFIWI
                     EGEHSALDRKDIQQHMIAAHAGGAASLVRLVNSDPDLVKPIMDMGTDIICFPFINTVE
                     DAQKAVAACTYPPNGIRGYNPQRASYYGNMNYFDYVKNANNETLKMMLIEQKAGYDNL
                     EEIVKVEGIDIIALGPGDLSLDMGFSGYMNRIEIKDMISHAAEICKKYKMPFIIFPTI
                     EKKSIEEWVNIGANMLCFAQDTTFITYVINQLLSFYNEIVPIQKQH"
     gene            complement(4662..5090)
                     /locus_tag="PC700_003011"
     CDS             complement(4662..5090)
                     /locus_tag="PC700_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDF2582287.1"
                     /translation="MSKIYIVYWSGTGNTEKMANFVAEGVKLKGKTPEVLDVSLLKPS
                     DLKEEDKFALGCPSMGAEQLEEGYMEPFVSELESMVSGKQIGLFGSYGWGNCEWMRDW
                     EERMQNAGATIIGGEGITAMEDPNEEAKDECIELGKTLAE"
     gene            complement(5566..6504)
                     /locus_tag="PC700_003012"
     CDS             complement(5566..6504)
                     /locus_tag="PC700_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HDF2582288.1"
                     /translation="MNKRMKLIPYEINENLRGAKNKFPYGIKQMNARGMWDEGYTGKN
                     IVVGIIDTGCDISHPLLKGKIIGGANFSDDSNGNKNIYEDFNGHGTHVAGIIAASNYN
                     NEVMGVAPDCKLLIAKALNKDGTGTYQSIINAINFAVNNKVDIISMSLGGNKDDKNLK
                     NAVMQAVKNNISVVCAAGNNGDGDSSTSEYSYPASYAEVIEVGAINENYLVEKFSNSN
                     TTIDLVAPGRNIISTYMDNKLAIMSGTSMSAPYVSGSLALIKEWAREEFERDLDEAEL
                     YAQLIKCTRALGIPRTEQGNGYLYLNLYKYKNNSKR"
BASE COUNT         2211 a         1099 c          653 g         2611 t
ORIGIN      
        1 gatttgaata tgaatttaaa gttatgtaaa attaaaaata caggaatgaa aattccattc
       61 ctgtataaaa tctaactttt gagtctcaat acaatcttat tttgagacaa tcttttttgt
      121 tttatatttt cttatattta aaatataacg catatcttat gcaacatatt catatttttc
      181 tttatattct ttcatcgcat cattccaagc gtcttgtact gttacacctc ttttattagc
      241 aattttttgt gccagttcaa ttatcttcat agctcttctt gatatgttca taaacattcc
      301 tcctacaaat taccatatat ttcatatata taaatcaagg ttatattttt atgatatata
      361 atccttctat tgtaatatta caatttatat gtgtattata tatgaactat attagaagat
      421 aattagaatt tttataaagc tatatgatta gattaaatat aaaagcttta caattactta
      481 taaatttaaa aagtctataa actaaactaa tgtttgatat accaatttta cgaccataaa
      541 taatgccatt actaaaaata ttggttttat catctttgaa cctttcttta tagcaacttt
      601 agcacctaca taagctccca ttatcatcac taatgcaaac actatagcaa ttttataatc
      661 tacttgacca tttatcatga ataataacaa tgaagttata ttacttgtta agtttaatat
      721 ttttgtattt gcagaagcat gtaaaaaatc atatccatat atttttataa atccaaaagt
      781 caaaaaagtt cctgtacctg gaccaaaaaa accatcataa aaacccatta taagagccat
      841 aagcatccct agtcttaaat ttttcttatt aacagcttca aaattatctt cttgacctaa
      901 atttttagaa ataaaggtgt aaatagccac tattaaaatc ataaccacaa ccaaaacatt
      961 taatatctct tcacttattg ataatacaca tctcacacct agaacactac caataatagt
     1021 aaatggtact aattttttta aaagatcgtt attaattttt ccagactttg catatctata
     1081 ggcactactt atacatcctg ctgatgctgc aaatttattt gtaccaatgg caagatgtac
     1141 tggcactcca attgccataa gaacaggcat gcttataagt ccctcctcct cctgctatag
     1201 aatctacaaa agcagctaaa aaccccccta tacataaaaa aataactgaa aacataaaat
     1261 tcctccatca ttctctttca atttttctta caatatatat tatataatct tatttaatac
     1321 ttattatcca gcatattctt tctatattca tttggagtta tactattaaa cttcttaaat
     1381 atttgtgaaa aataactact gttattaaaa cctactttct tacaaatatc actaatactc
     1441 atgcttctat ctaacagata ttttttagat tcttctatcc taagaagatt tatatactca
     1501 gttattgaaa ctttatttct ttcattaaaa atagaactca aataatttgg acttataaaa
     1561 aaaacatctg atatactatt caaatttata ttttctttat aatatttttt tatatattcg
     1621 tttatatttt caattagatt tcctttatct tttctccaaa atgctctatt cttatcaata
     1681 cactctttgg aaaattcaaa taatacattc cataaatctt catcactgtc tacaagtgat
     1741 atttttttat tatatttatt tcttaaataa gatataagtt ttaaatcctc taaatctcta
     1801 agcactgttc tagacaatac aaatatcatt tcctgaatag tatctctaac atcattatat
     1861 cttaatttgt ttgtatctat tagattataa acaccttcta aattcatatt tttaatttct
     1921 ttttcaatat aaagttctaa ttcaaaggga tattcttttg aagaattatt gaacatatct
     1981 atcggattaa taatatactc ttcttctaat aatctactat ttgctttttt aagtgcctct
     2041 tcaagtctta tattttcttt tctactcttt aatatctcct catcttttat actaagttga
     2101 ttttcaaaaa aattcctctt agctatttcc tgaataaatt cagacatcat taataaattt
     2161 tcttttaata catataatct acttttagga tttaatggaa attcattgta aattttgttt
     2221 atgtatttta tatcaatacc agaactactt gataattcag ataaattttt atctatatca
     2281 atagacttat atactataaa attaccacat tctatatatc caactatatt ttcttctaat
     2341 actataggta tctctaaact ggaaacacca tactcacaat ggtacacttt ttcaaagtta
     2401 tcattattta tgcttttaac aagtttttta gtacacggtt ttatattttt acaaatatcg
     2461 caaaatttat tttctttctt agtctcaaat attcctttat actttaagtc atatacagat
     2521 attctaagct tcaatatatt agatatttga tttgcaagat tttcaattat gtttacatta
     2581 actaactctt gtatattgtt ttcagaaaat actataggta tttgtctcaa actcgaaaat
     2641 ttatgaggaa cataagtaat taatattact aaatcaattt taccagtgtt tataatttca
     2701 tgttctgaat atggtgggca ataaactata gatgtttcct ttatattgac ctttttacca
     2761 tcaatcattt gtattccttc tccctgaatc acatataaaa gctgttcttc acttaaatga
     2821 aaatgctttt cttggcagtt tcccggaaaa aatcttacaa ttccagcact aagtctattg
     2881 aattgtgttt ttgatggttc gtgtatccac ataacctctc cccaatcaaa tttttgtact
     2941 tcattatttg actcaataaa atccaccata aactcacctc gtagttttta tctaaatatt
     3001 tttttattcc tttaaatatc tccttataat atacattatc ataattttct tttttattgt
     3061 atagataaat agttatatag aacattgtcc aattcttcct aaaaatatag tgtaaaaaat
     3121 ctttcctcaa atgtttccaa ttgaatttac tctttatatt agaaaaatca aaaaattatt
     3181 tctttattct ataaggatta ttttttacac atcttttttt cttttaagta atatattttc
     3241 tcatctattt tatccataca actttctagt tcatgtattt tatttttaat gttttgctta
     3301 tgttgttgaa ataattggat tcttttatct attgtaccat ctcccatatc acaaaaatga
     3361 aagcattcat ggatatcttt aatagataaa ccagtatttt ttaattcttt gataaatatt
     3421 agacgctcta catcttcttc agagaaatag cgaacaccac ttggttttct attgatagga
     3481 agtaataaac ctttttcttc ataaaatcga atagcagatg aagaaatttt tgttattttt
     3541 gatacatcag ttatagaata tttcataatc tctacctccc taaattttat atgaataaga
     3601 atattataaa aataccttat acagtagtca acaaaaaagt attgacttca agttaggttg
     3661 aagttgtact ctttgaagtg taaaagaaaa cttacaaaag aaaggatgat ataaatggaa
     3721 aaatatgcaa ctatgattaa agaaaagcta aatgaaggaa aggtcttatg tggagtagct
     3781 gtttcactgt ctgattcagt tgtaagtgag ttaatcggta tatctggata tgattttatt
     3841 tggattgaag gagaacatag tgctttagac cgtaaagaca ttcagcaaca tatgatagct
     3901 gcccatgctg gtggtgctgc ttctttggta cgattagtaa acagtgaccc tgaccttgtt
     3961 aagcccataa tggatatggg aacagatatt atttgtttcc catttattaa tacagtagaa
     4021 gatgcacaaa aagccgtagc agcttgtact tatcctccaa acggtataag aggatataat
     4081 cctcaaagag cctcttatta tgggaatatg aattattttg actatgtcaa aaacgcaaac
     4141 aatgaaacat taaaaatgat gttaattgaa cagaaagcag gttatgataa tcttgaagaa
     4201 atagtaaaag tcgagggtat tgatattata gcattaggcc ctggtgactt atctttagac
     4261 atgggttttt ccggatatat gaatagaatt gaaataaaag atatgatttc tcatgctgct
     4321 gaaatatgca aaaaatataa gatgcccttt attatttttc ctacaataga aaagaaaagt
     4381 attgaagaat gggttaatat tggagcaaat atgctttgtt ttgcacaaga tactaccttt
     4441 atcacttatg ttatcaatca attattatct ttttataatg agattgtacc aattcaaaaa
     4501 caacattaaa ttataaatct aattttatat taaaagagac taatatttta tctatctcta
     4561 ttaaggatat tttcttatgt atcataatat tagtctctct tattattata tactgctatc
     4621 atcattagtt gctatttata ctatttttat aatatacaaa tttattcagc taacgttttg
     4681 cctaattcta tacactcatc ttttgcttct tcatttgggt cttccattgc tgtaattcct
     4741 tctccaccaa taattgtagc accagcattt tgcatacgtt cttcccagtc tctcatccat
     4801 tcacaatttc cccatccata tgaaccaaat aatccaatct gtttacctga taccatagat
     4861 tctaattctg aaacaaatgg ctccatatac ccctcttcta gttgctctgc tcccatagat
     4921 gggcaaccta atgcaaattt atcttcttct tttaaatcac ttggtttcag taagctcaca
     4981 tctaaaactt ctggtgtctt accttttaac ttcacacctt cagccacaaa atttgccatt
     5041 ttttcagtat ttcctgttcc actccaataa acaatatata ttttactcat ttttacctcc
     5101 tgtagtattt tttaattaat caatagttag ttatagctaa ctaacatcta aaaaacaaaa
     5161 gatttcttta tctgaaatct ctttattttt ctaaatatag aatatcacaa ttgattttga
     5221 aattcaatat ttattgataa tatttatcat ttatatttta tattcatttt ataatatcaa
     5281 aacactgtat cgtatttcta atctttcata gaaaaatttc taacttatag tacttttgca
     5341 tctcttataa atcttttata tgtataagta catagaaaaa cacacctttt ccagatgtgc
     5401 tatctataat atcgtatatt tatttttcaa ttctaactag ctaatatgtt tataaatttt
     5461 atcacttaat acctttatac attgatgtaa aatcttacac ctaagtaatt tttattattt
     5521 gtctcgactc tatataatat agctatttta tcatcaaatc aaaaattatc ttttgctatt
     5581 attcttgtat ttataaagat ttaaatataa gtatccattt ccttgttccg ttctaggtat
     5641 tccaagcgct ctcgtacatt ttattaattg tgcatacagt tcagcttcat ctaaatctct
     5701 ttcaaactcc tctcttgccc attctttaat tagtgctaat gagcctgata cgtatggtgc
     5761 actcatacta gtaccactca taatagcaag tttattatcc atataagtcg atataatatt
     5821 tcttcctgga gccaccaaat ctattgtagt atttgaatta ctaaactttt caaccaaata
     5881 gttttcattt attgcaccta cttctattac ctcagcataa ctggctggat aactatactc
     5941 acttgtacta gaatcaccat ctccattatt acctgctgca cacactacag aaatattatt
     6001 ttttactgct tgcatgacag cattttttaa attcttatca tctttgtttc ccccaagaga
     6061 catagatata atatcaacct tgttatttac agcaaagtta atagcattaa ttatactttg
     6121 atatgttccg gtaccatctt tatttaatgc ttttgctatt aataatttac aatctggagc
     6181 tactcccata acttcattat tatagttaga tgcagctata atacccgcca catgagttcc
     6241 atggccatta aaatcctcat atatattttt atttccatta ctgtcatcac taaaatttgc
     6301 accaccaatt atttttcctt ttaaaagagg atgagatata tcacaacctg tatctattat
     6361 accaactaca atatttttac cagtataacc ttcatcccac attcccctag cattcatttg
     6421 ttttattcca tatgggaatt tattttttgc acctcttaga ttttcattta tttcatacgg
     6481 aattagtttc attcttttat tcatcatata tcctcctaaa aattttattg cttataaaaa
     6541 tcatgaattt tattttataa taggtttata aatc
//
LOCUS       DAMJWP010000196         6536 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.203, whole genome shotgun sequence.
ACCESSION   DAMJWP010000196 DAMJWP010000000
VERSION     DAMJWP010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6536
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.203"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            118..426
                     /locus_tag="PC700_003013"
     CDS             118..426
                     /locus_tag="PC700_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582289.1"
                     /translation="MFNKIYEKKEFIVFQVKKGYVVYNTRKSFEEGHTHLKHFEAAKT
                     AIDLAINKKIPRSKDGYYLTSLIRISDDGYYIDKLSELLYVREQKGKKEKYCNSGYQM
                     "
     gene            complement(588..2378)
                     /locus_tag="PC700_003014"
     CDS             complement(588..2378)
                     /locus_tag="PC700_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2582290.1"
                     /translation="MKKSKGFKKTIGRLLPFVKKYKFSFIIAIICIISAATMNALAPK
                     TEGLIITQLTKDVISIAKGVPGASVNFDYVTKILVILACIYFANAIFTYTSSFLLTNA
                     IQNTMRDLRNEVENKIRRLPISYFDSNSFGDVLSRISNDVDTISNALQQSFMQIVNSI
                     LVIILALSMMFTINIYMALIALFIIPISYFVSKFVVKKSQSRFSLQQNALGKLNGKVQ
                     EMYTGFNEIKLYGKEEDSIKEFKKVNQELCENGFKAQFISSMMNPMVSLVTYFGIAAV
                     AVVGSIYAVSGGITVGNLQAFVRYIWQINQPLSQMTQLSTVIQSSFAAIERVFEILDE
                     EEEIPDVENPVKIENVKGNVTFEHVNFGYGENSTLIEDLNAEVKSGQMVAIVGPTGAG
                     KTTLINLLMRFYDVKKGAIKIDGVDIRDMKRKDLRSMFGMVLQDTWLFNGTIFENIEY
                     GRFGATKEEIIQAAKVANVHHFITTLPDGYNMFLNEEASNISLGEKQLLTIARAFISD
                     PSILILDEATSSVDTRLELMLQKAMRNLMNGRTSFVIAHRLSTIRNADLILVMNNGSI
                     IEQGNHDELMEKGGFYEKLYNSQFADKESE"
     gene            complement(2378..4105)
                     /locus_tag="PC700_003015"
     CDS             complement(2378..4105)
                     /locus_tag="PC700_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003496244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2582291.1"
                     /translation="MKLILSYLKNYKLLIVLNILAIFSFALVELGIPTIIAKIIDNGI
                     ANQNIAYIKQMGIVIVIISIIGVVGSILLGYCSAKVSTSVTRDIRNDIFEKSQEFSHT
                     EYNKFGISSMITRTTNDAFQIQQFVNILLRTALLTPVMFIISIIMTIRTSVELSIVLA
                     ISVPFIIIGVAIIAKVSQAISSKQQKGLDKLNLISRENLTGIRVIRAFGNDDYETERF
                     EKTNTYYANVSKKLFKLMSITQPAFFLLLNIAVLAVFWISSEKINIGELQVGQLVAFL
                     EYLFHAMFSIMLFSMVFIMYPRAEVSANRIKELLNEEPLIKNPENGIKDTENKGIIEF
                     DNVTFTYPDGEASVLKDISFTAKTGETVAFIGSTGSGKSTLINLIPRFYDVTEGSIKI
                     NGVDIREYDLKALRKKIGFIPQKSLLFTGSIANNIRFGKHKAGESELEYSARVAQAYE
                     FISKKPRKFDELISEGGANVSGGQKQRLSIARAIIRRPEIYIFDDSFSALDFKTDAIL
                     RAKLKKETKDAIVLIVAQRISSIIDADKIIVLNEGQVVGMGTHKELLKNCEIYYEIAT
                     SQLKKEELE"
     gene            complement(4350..4914)
                     /locus_tag="PC700_003016"
                     /pseudo
     CDS             complement(4350..4914)
                     /locus_tag="PC700_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
     gene            5203..6315
                     /locus_tag="PC700_003017"
     CDS             5203..6315
                     /locus_tag="PC700_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDF2582292.1"
                     /translation="MKISEVATPNFLLDLDQLEKNIDKIQQICTNNNKQLWPMLKTHK
                     STYIARLQKNAGASGFLVGTLDEGEALIKAGFEDIMLAYPYMGEVNINRIVTMASKIN
                     LICATDNIECAKAYSEAFSKSKIECKLLIIVDCGLHRFGVEPEKVVELATEISKLENI
                     KIVGISSHPGQVYGCSSPEGVPDVCVQEDSSMEKAYKALTDNGFNIEIVASGSTPTVE
                     AEAASKTITAVRPGNYVYYDAIQIGLGCATEDMCALTVVCTVISKNSSGYYLIDCGSK
                     CLGLDKGAHGNSSIVGYGRVCGHPELTIDSLSEEVGKIKVNGETDIKIGDRIRIIPNH
                     SCSTANLTSNLLGFRGDTIEKVIEVDIRENSKQIIL"
BASE COUNT         2241 a         1038 c          841 g         2416 t
ORIGIN      
        1 tatctttata aattatagtt ttagatttat aatttataaa atatttatgt tctttgtaaa
       61 attttttaga agcttctaat gttatgctat aaaactatct tgagaagggc tgattgaatg
      121 tttaataaaa tatatgaaaa gaaagaattt atagtttttc aagttaaaaa aggttatgta
      181 gtttataata ctagaaagag ttttgaagaa ggtcatacac atttaaagca ttttgaagca
      241 gcaaagacag ctattgattt agcaataaat aaaaagattc ctagaagcaa agatggatat
      301 tatctgacta gtttaattcg cataagtgat gatggttatt acattgataa gttaagcgaa
      361 ttgttatatg ttagagaaca aaaaggtaaa aaagaaaagt actgtaattc tggttatcag
      421 atgtaacaag actagttgtg aattataagt accaatcata aataccttta gtcttaaaat
      481 caaagagtat cagtaaatct ataaaaaaac aacttactga tactcttttt aattatatga
      541 ttaatatttg aatttgatac aaaattttat acaaaagttt atgttatcta ttctgattct
      601 ttgtctgcaa attggctatt ataaagcttc tcataaaatc cacccttttc cattaattca
      661 tcatgattcc cctgctctat aatacttcca ttattcataa ctaaaatcaa gtcagcattg
      721 cgaattgtag aaagtctatg tgctataaca aaactagttc taccattcat aagatttctc
      781 attgcttttt gaagcataag ttccagccta gtatcaacag aactagtagc ttcatcaagt
      841 atcaatattg aagggtctga tataaaagct ctagctatgg ttaaaagctg tttttcccct
      901 agagatatat tagatgcttc ttcatttaag aacatattgt atccatctgg aagagtggtt
      961 ataaagtgat gaacatttgc tacttttgct gcttgtataa tttcttcttt agtagctcca
     1021 aatctaccat attctatatt ttcaaaaatg gttccattaa ataaccatgt atcttgaagc
     1081 accataccaa acattgaacg caaatcttta cgtttcatat ctcttatatc tactccatca
     1141 attttaatag cacctttctt tacatcataa aacctcatca ataagtttat taatgttgtt
     1201 ttacctgctc cagtaggtcc aacaatagct accatctgac cactttttac ttctgcattt
     1261 aaatcttcta ttaaagttga attttctcca tatccaaaat taacatgctc aaatgtaaca
     1321 tttcctttta cattttctat cttaactgga ttttcaacat caggtatttc ctcttcttcg
     1381 tctaaaattt caaatacacg ttctatagct gcaaaagaag attgtattac tgtagataac
     1441 tgagtcattt gtgaaagtgg ctgatttatc tgccatatat aacgcacaaa tgcttgtaaa
     1501 tttcctactg ttatccctcc acttactgca tatatagaac cgacaactgc cacagctgca
     1561 attccaaagt aagttactaa tgaaaccata ggattcatca tacttgatat aaattgtgcc
     1621 ttaaatccat tttcacaaag ttcttgattt acttttttaa attcttttat agaatcttct
     1681 tctttaccat atagttttat ttcattaaat ccagtataca tttcttgtac ttttccattt
     1741 aattttccaa gagcattttg ttgcaatgaa aatctacttt gagatttttt tactacaaat
     1801 ttagatacaa aataactaat tggaattata aaaagagcta taagagccat atatatatta
     1861 attgtaaaca tcattgaaag tgccaaaatt atgactaaga ttgagtttac aatttgcata
     1921 aaactctgtt gcaatgcatt tgatattgta tctacatcat ttgatatacg acttaataca
     1981 tctccaaaac tattactatc aaaataacta ataggcaatc ttcttatttt attttcgact
     2041 tcatttctta aatctctcat tgtgttttga atagcattcg taagtagaaa acttgaagta
     2101 tacgtaaaaa ttgcattagc aaaatatata catgctaaaa tcaccaatat ttttgttaca
     2161 taatcaaaat ttacggaagc ccctggtact ccttttgcga tactaattac atctttagtc
     2221 aattgtgtta ttatcaatcc ttcagtttta ggtgcaagtg cattcatagt agctgctgaa
     2281 attatacata taatagctat aataaaggaa aatttatatt tctttacaaa tggaagaagt
     2341 ctaccaattg ttttcttaaa acctttagac tttttcatta ttctaattcc tcctttttta
     2401 actgagatgt tgcaatttca tagtatattt cacagttttt taataattct ttatgagttc
     2461 ccattccgac aacttgtcct tcattgagta ctataatctt atcagcatca ataatagaac
     2521 taattctctg ggcaacaatc aatacaattg catcttttgt ttccttcttc aatttagcac
     2581 gaagtattgc atccgtttta aagtcaagtg cagaaaaact atcatcaaaa atgtatattt
     2641 caggtcttct tataatagcc cttgcaatag aaagtctttg tttttgacct cctgatacat
     2701 ttgctccacc ttcactaatc aattcatcaa actttctagg tttttttgat ataaactcat
     2761 atgcttgtgc aactcttgcg gaatattcta attctgactc tcctgcctta tgtttcccaa
     2821 atctaatatt atttgctatg ctaccagtaa aaagcagtga tttttgaggt atgaatccta
     2881 tcttttttct taaagctttt aaatcatatt ctcttatatc tactccattt atcttaatgc
     2941 taccctctgt tacatcgtaa aatctaggta tcaagttaat tagagtactt ttcccactac
     3001 cagtacttcc tataaaagcg actgtttcac ctgtttttgc agtaaatgaa atatccttaa
     3061 gtacagaagc ttctccatct ggataggtaa aagttacatt atcaaattct atgataccct
     3121 tattttcagt atccttaatt ccattttcag gattttttat caatggttct tcatttaaaa
     3181 gttcttttat tctatttgca gatacctctg ctcttggata cataataaat accattgaaa
     3241 aaagcataat tgaaaacatg gcatggaaaa gatattccaa aaatgcaacc aactgtccaa
     3301 cttgaagctc tccaatattt atcttctcac ttgaaatcca aaatacagcc aaaacagcta
     3361 tatttaatag taaaaagaat gctggttgag taattgacat aagcttaaat agtttcttag
     3421 aaacattagc ataatatgta tttgtttttt caaatcgctc tgtttcataa tcatcatttc
     3481 caaaagctct aattactctt attccagtta aattttctct cgatataagg tttaatttat
     3541 ctagtccttt ttgttgtttt gaagatattg cctgagatac ttttgctatt attgctactc
     3601 caataattat aaaaggtact gatatagcca atactataga aagttccaca cttgttctaa
     3661 ttgtcattat tatacttatg atgaacataa ctggagttaa taaggcagtc cttagtagta
     3721 tattaacaaa ttgttgaatt tgaaatgcat cattcgttgt tcttgtaatc attgaagaaa
     3781 ttccaaattt attgtattca gtatgtgaaa attcttgtga tttctcaaat atgtcatttc
     3841 ttatatctct ggttacactt gttgatacct tagcagaaca ataacccaac aatatactac
     3901 ctacaacccc tataatagaa ataataacaa tcactattcc catttgtttt atgtaagcta
     3961 tattctgatt tgcaatacca ttgtcaatta ttttagctat tatagttgga atccccaact
     4021 cgactaatgc aaaactaaat atagccagta tgtttaatac tattaataac ttataattct
     4081 ttaaataact taaaattaat ttcatatttt tctcctttct taatttttga gatagtgtaa
     4141 tcatgtaata atactaaatt tataaatgct taattttata aaaaaaggag aaaagagtta
     4201 acgcactctc ttctccagca tattcttttt aatacaccct ccgacaagtc gcttatatgt
     4261 taacatattt gacaagtaat gtcaattcat atagctaaaa gtcttttaaa tgcttcatct
     4321 cataacattg taacaaattg gcatgtaatt tatttttgtt ttttactgta ttccaatttt
     4381 acaagttctc caatattact aactttctta aaattcaaat tagtcttatc tataccagac
     4441 ttaaatagtg aaatccctct ccccaatatt attggcaata tatatatata ataatcatca
     4501 attaagttat ctttcataaa taattttact atatcgcctc caccaactat ccaaatatct
     4561 tttttatcct tcttttttcc aatttgctca attaattttt taggttctaa attagtatac
     4621 tttacattct ccagttgtcc cattaattgt atttgagtat atatagcatt ccttaccctt
     4681 atagtaccat tcatttgggc ttaattcatt tgcaatctgt acatatgtgg aatttcccat
     4741 aatgatagta tcaattgtat cgtaaaaagt attataatca tcttcctgct cattcttaaa
     4801 ttctgaagat atctcgtcta aaaaacttac actgccattc tcttcagcta tataaccatc
     4861 taaacttgtg gcaatataaa gtactacttt tctcatgttt actacctcca acatttatag
     4921 tgtgattatt ctatgtaaac atcacttttt tactcattca ataataccct attaataaat
     4981 cctcaaacta atttaaataa actttgcata aaactaaaaa tatattatat aaataattta
     5041 aaatcacact aacttattga aaattctaat tattacaaat aaatattgct ctctgttaac
     5101 gctagtttta taatataatt ataagctatt aatattataa agccgatatt actaatataa
     5161 taattatagt taatttactt ttgttgaaag gtggtaaata acatgaaaat ttcagaagtc
     5221 gctactccaa attttttgct agatttagac cagttagaaa agaatataga taaaattcaa
     5281 caaatttgta caaataataa taaacaactt tggcctatgc ttaagacaca taaaagtact
     5341 tacatagcga gacttcaaaa aaatgcaggt gctagtggtt ttctagttgg aacacttgat
     5401 gaaggtgaag ctctgataaa agcaggtttt gaagatatta tgttggctta tccttacatg
     5461 ggagaagtaa atataaatag aattgtgact atggcatcaa aaataaattt aatctgtgct
     5521 acagataata tagaatgtgc taaggcttat agtgaagctt tttctaaatc aaaaatagag
     5581 tgcaagctac ttattattgt agattgtggc ttacaccgtt ttggggttga accagaaaaa
     5641 gtggttgaac ttgctactga aatttctaag ctagaaaaca tcaagattgt tggtataagt
     5701 tcgcatccag gtcaagtgta cggttgtagt tctccagaag gtgttccaga tgtttgtgtt
     5761 caagaagatt cttctatgga gaaagcatac aaagctctaa ctgataatgg atttaatata
     5821 gaaattgtcg cttctggtag tactccaaca gttgaagctg aagctgcttc taaaactata
     5881 actgctgtaa gacctggaaa ctatgtatac tatgatgcta tacagatagg tttaggttgt
     5941 gctactgaag atatgtgtgc tcttactgtt gtgtgtactg taatctcaaa aaatagcagt
     6001 ggatattatt taattgattg tggaagtaag tgccttggac ttgataaagg tgcacatgga
     6061 aatagtagta ttgttggtta tggtagggta tgtggacatc cagaacttac tattgattca
     6121 ctttcagaag aagttggaaa aattaaagta aatggtgaaa ctgatataaa aattggtgat
     6181 agaataagaa ttatacctaa tcattcttgt tccactgcaa acttgacgag taatttatta
     6241 ggttttagag gagatacaat agaaaaagtc atagaagtag atattcgtga aaattcaaaa
     6301 caaatcatcc tttaaaaata tatttatttt gatattaaca tcatgaaagt atatttttct
     6361 taaaattatt gttaataact aatgttaatg ttggataaat aaaaaacata aattctagaa
     6421 atataaattt aaaaaaagag gctgtctcaa aattggatat gtccctgtca agtagacaga
     6481 cttaaaaaag gctatcttta tgcggaatga gttcgatatt caatcggact cattcc
//
LOCUS       DAMJWP010000197         6528 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.204, whole genome shotgun sequence.
ACCESSION   DAMJWP010000197 DAMJWP010000000
VERSION     DAMJWP010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6528
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.204"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(<1..656)
                     /locus_tag="PC700_003018"
     CDS             complement(<1..656)
                     /locus_tag="PC700_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HDF2582293.1"
                     /translation="MKKDTSHIIKRAEHFQVILIGEGLMVGAVAGLIVLLYRILLGQA
                     GTWLSQILKFVEGSTIKIIAWFAVLAVLAWIVSKLVDWEPMISGSGIPQLEGEMTGKI
                     KQVWWRVLPTKFIGGFLSLMGGLALGREGPSIQLGAMAGKGISRALDRGKTEEKFLLT
                     CGASAGLSAAFHAPLAGVMFSLEEVHKNFSVSVLISVMTASLTADYISANFLGMESVF
                     "
     gene            complement(843..1352)
                     /locus_tag="PC700_003019"
     CDS             complement(843..1352)
                     /locus_tag="PC700_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HDF2582294.1"
                     /translation="MIGLKCGMVKLLEHQMIWDKSAKDVIILLKSIWDKTAIDIQHIG
                     STSIPSISAKPIIDIVVGVASLEEAKLYLERLEQCGIVFRGQDVPKQLLFAMGDFEKN
                     TRTHHIHVVEWNSVAWNNYINFRDYLNTFTDKAKLYDSHKQKLALQFEDNRKIYTQRK
                     QELINILLE"
     gene            complement(1427..1840)
                     /locus_tag="PC700_003020"
     CDS             complement(1427..1840)
                     /locus_tag="PC700_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HDF2582295.1"
                     /translation="MKMPKESIDTIMFAPCGMNCMVCYKHCYHKKPCAGCLNSDTGKP
                     EHCRKCKIKDCVCQKGLFYCFECFNFPCKLIKNLEKSYNKRYQASLMENSKFVQQYGL
                     EKFMQKQKERYTCSKCGGIISIHDRECSECQEKVK"
     gene            2277..2936
                     /locus_tag="PC700_003021"
     CDS             2277..2936
                     /locus_tag="PC700_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HDF2582296.1"
                     /translation="MDEKILELLQEIQGNISNMQGSISGIEGTVNGMQGNINYMQGNI
                     NYMQGTISDMQGTISDMQGSINGIEGTISDMQGSINGIEGTISDMQSNINDIEGTISD
                     MQGSISSMDGTINSMQDSITDMQGEIKEVNSRLDNMEPDIKDLKTGQVETSSRLDTIQ
                     SDVTEIKSDVKINRNTLDTVIKHTSSLVEDTSDIKNDLNNIEIVTSKNWNDIAKLKSV
                     K"
     gene            complement(3543..3722)
                     /locus_tag="PC700_003022"
     CDS             complement(3543..3722)
                     /locus_tag="PC700_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2582297.1"
                     /translation="MLQTGKQGADKKYLDNMFFVKGYVEELLFLDNSYDIVFSCIVFH
                     HCTNVQNIFCIFWKW"
     gene            complement(3947..4480)
                     /locus_tag="PC700_003023"
     CDS             complement(3947..4480)
                     /locus_tag="PC700_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDF2582298.1"
                     /translation="MQYISYYHSPIGNILLASDNVGLTGLWFENQKYYAYNLDQEHQE
                     KELPIFEQTKKWLDIYFSGEEPNFTPSLHMVGTPFQTTVWKILQQIPYGKTITYGEIA
                     HKVAQQKGISKMSAQAIGGAVGHNGISIIVPCHRVVGTNGSLTGYAGGIDRKIKLLSL
                     EKVSVQHYFIPKKGSAL"
     gene            complement(5298..5870)
                     /locus_tag="PC700_003024"
     CDS             complement(5298..5870)
                     /locus_tag="PC700_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDF2582299.1"
                     /translation="MWIYQKTLEHPVNIRQADPRMAKYIMTQLGGPNGELAAATRYLQ
                     QRYTMPTGKSRALLTDIGTEEMAHVEIISSVLYQLIGSCTPEELKAAGLGSNYANFGH
                     GLQPVDSNGVNFTTSYINVFGDSVTDLHEDMAAEQKALATYYQLINLTDDPDLKDILR
                     FLGEREVVHYQRFGEALMDVYEFTECKHQF"
     gene            complement(5932..6195)
                     /locus_tag="PC700_003025"
     CDS             complement(5932..6195)
                     /locus_tag="PC700_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HDF2582300.1"
                     /translation="MSLNRRELLERISEYQFACIELNLYLDNNPRDKKALDLYNRYCD
                     KFTQAVCDYESKYGALTNFGYESSEYPWSWISEPWPWDKSFYK"
     gene            complement(6195..6416)
                     /locus_tag="PC700_003026"
     CDS             complement(6195..6416)
                     /locus_tag="PC700_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HDF2582301.1"
                     /translation="MGYLKEDKQDYCCEFARPYINRQIYDDTYNPKTALKRGTLFPEL
                     DLIESNNYNDWLYSNPKNRVRAKKGGNMY"
BASE COUNT         2288 a         1126 c          810 g         2304 t
ORIGIN      
        1 tggaatacag actccattcc taggaaattg gctgaaatat agtctgctgt caaagatgct
       61 gtcatcacag aaattaaaac agatactgaa aagtttttat ggacttcttc aagtgaaaac
      121 ataacacctg ccagtggtgc atgaaaggct gctgataatc cagcacttgc cccacaggtt
      181 aacaggaatt tttcctctgt cttccctcgg tcaagagctc tagaaatacc tttacctgcc
      241 atagcaccaa gctgaatgga aggaccttcc cttcccaaag caagaccacc cataagtgat
      301 aagaatcctc caataaattt tgtgggcaac actcgccacc atacttgttt tattttccct
      361 gtcatttccc cctcaagctg agggatacca cttccagaaa tcattggctc ccagtccacc
      421 aactttgata caatccacgc aagtactgca agtactgcaa accaagctat tatttttatt
      481 gtagaacctt ctacaaattt taaaatttgg ctaagccaag taccagcctg ccctaaaagt
      541 atcctgtata aaagcacaat caaaccagcc acagccccaa ccataagtcc ttcaccaatc
      601 aaaattacct gaaagtgctc tgcccgtttg ataatatgag atgtatcctt tttcatactg
      661 ttcctccttc atccaataat tctacacaat ctgaacctcc aatgactaaa gtcatgagtg
      721 tgtacagata ttttttaatc aacaaaaata gatttttatt tactaaatat aagaaaataa
      781 ttgttcaatt tctcaataaa tgtaaattta ttcttttagg tatttattct ccacagtcgt
      841 gcttattcca gcaaaatatt tattagttct tgctttctct gtgtatagat tttacgattg
      901 tcctcaaact gtaatgctaa tttttgttta tgactatcat agagttttgc tttgtctgta
      961 aaagtattta gataatctcg aaaattgata taattattcc atgcgacact attccattca
     1021 acaacatgaa tatggtgagt acgagtattt ttctcaaaat ctcccatagc aaaaagtaat
     1081 tgttttggta catcctgacc tcgaaaaacg attccacatt gctctaacct ttcgaggtat
     1141 aatttagctt cctctaaaga agcaacacct acaacaatat caataatagg ctttgcgcta
     1201 atactgggaa tagaagtgct tccaatgtgt tgaatatcaa tggcagtctt atcccatata
     1261 cttttcaata atataatgac atcttttgca cttttatccc atatcatctg atgttccaat
     1321 aatttaacca taccacattt taaacctatc actatatacc acctcaatat aatccacctt
     1381 tatatatata ttacaatttt ttctatcttg ataaacagtg atctagctat ttcacttttt
     1441 cttgacactc gctacattcc ctatcatgaa tagatataat tcctccacat ttagaacaag
     1501 tatatctctc cttctgcttt tgcataaact tctctaaacc gtactgctga acaaatttac
     1561 tattttccat aaggcttgct tgatatctct tgttataact tttttcaaga ttttttatga
     1621 gtttacaagg aaaattgaaa cattcaaaac aataaaacaa ccctttttga cagacacaat
     1681 cctttatttt acacttacgg cagtgttctg gctttcctgt atcgctgttc aaacaaccag
     1741 cacatggctt tttgtgataa caatgcttat agcaaaccat acaattcatt ccacaaggag
     1801 caaacataat cgtatcaata ctctcttttg gcattttcat aacattttac tcctttcaaa
     1861 aatccaattt gtcgaattgt tatatattca tattgtaaca taaaaaatag ctaaaaaatt
     1921 catgcacttt cattgccaat aaattttcaa ggaaatatga aagtttaaaa ctaagaataa
     1981 aaagaaataa aaaggaagct actaatcaaa atattcaaac acaagtaatc aattatgata
     2041 ttaccagcag ttttaaatac aaatataacc acatatgcct caaaaacact tattatagta
     2101 tcttagacac ttagacaata cacaagattc attaataaaa aagttctcaa tatagatata
     2161 tttaaccatg ttttgaatag cagataatta tatgattaaa aattaaatat gacatagcgt
     2221 ttgtggaata taacacctaa attatgttat aataataaaa caggagtgtg atagtgatgg
     2281 atgaaaaaat attagaatta ttacaggaaa tacaaggtaa tattagtaat atgcaaggta
     2341 gtatcagtgg tatcgaaggt actgttaatg gcatgcaggg taatattaac tatatgcagg
     2401 gtaatattaa ctatatgcaa ggtactatta gcgatatgca aggtactatt agcgatatgc
     2461 aaggcagtat caatggtatc gaaggcacta ttagcgatat gcaaggcagt atcaatggta
     2521 tcgaaggcac tattagcgat atgcaaagca atatcaatga tatcgaaggt actattagcg
     2581 atatgcaagg cagtatcagt agtatggatg gcactattaa tagtatgcaa gacagtatta
     2641 ctgatatgca aggtgaaatt aaagaagtca atagtagatt agacaatatg gaacctgata
     2701 ttaaagactt aaaaactgga caagttgaaa ctagtagtag acttgataca attcagagtg
     2761 atgttacaga aattaagtca gatgtaaaaa ttaacagaaa tacattagat acagtgatta
     2821 aacatacctc ttcattagta gaagatacat cagatattaa aaatgattta aacaatatag
     2881 agatagtcac atctaagaat tggaatgata ttgctaaatt aaaatcagtt aaataatata
     2941 ccatagtaaa aaatacctga aaatttcaca tacttcttta aagttatacc aaattttata
     3001 cagaaatact gattatccga caacacactt tacttataaa taatttatta aaaaatactc
     3061 attttatcct aaaattaaca cgactaaatg agtcttgata taataataaa aaataaggga
     3121 gattgaacac taaaaagttg tgtatctccc ttatattcta taatatagtt atataatttt
     3181 tcagcctaaa ataaaagtcc atctttgatt aaaacatatc cccttatcac ttataaaagg
     3241 ataaaatctt gttttcattt ttccatttat atcttcttgc ttaagagtaa taattttttc
     3301 ttttgatact ttaagtgttt ggttaaattt aaccaatcga ctaagttttg ttcaacatct
     3361 atttttcata ttttccaata cttaatctat tttttataaa taattctttt atttctaact
     3421 cacttacatt ttttacataa aacgaagcat ataaatttca accttatctt gtatggttct
     3481 taatacttaa tgagtagctt aagtatcaat cattataaat tttcccctta tatttaacac
     3541 tcttaccatt tccaaaatat acaaaatata ttttgaacat ttgtacaatg atgaaataca
     3601 atacatgaaa atacaatatc ataactgtta tctaaaaata gaagttcttc aacataacct
     3661 ttcacaaaga acatattatc taagtatttt ttgtctgctc cttgttttcc tgtttgtagc
     3721 atataaatta ctgcatctaa acatataact actcacacga aaaaagaaaa taaacttcca
     3781 aaatacaagc tctaaaacta cctctaaagt tatataatgt ttactaggaa taaggttgta
     3841 taacatatag tttaaagaat agttttttaa aattgcaagt tattgctttc aaaattagat
     3901 gtttatatat caaataaatg attaagaatt tctaaattta atggctttat aatgcactcc
     3961 ctttctttgg gataaaataa tgttgcacag atactttttc taaagataac aattttattt
     4021 ttctatcaat tccaccagca taccctgtca aactcccatt tgttcctaca acacgatgac
     4081 atggaacaat aatagagatt ccattatgcc ctactgcacc accgattgct tgtgctgaca
     4141 ttttagatat tcctttttgt tgagcaactt tatgtgcaat ttctccataa gtgattgttt
     4201 taccatatgg tatttgttgt aaaattttcc acactgtagt ctggaaagga gtacctacca
     4261 tatggagtga tggtgtaaaa ttgggctctt ctcccgaaaa ataaatatct agccatttct
     4321 ttgtctgctc gaaaattggc aattcttttt cttgatgttc ttggtcaaga ttataagcat
     4381 aatacttttg attctcaaac caaagaccag ttaacccaac attatctgat gctaaaagaa
     4441 tatttccaat tggactatgg taatagctaa tatattgcat acaattcctc cttatattct
     4501 gaaaacttca ctcaaatcat tagacacttc ttttctatct gccattatgt aaaaacatac
     4561 taatataatt tccttctgac tacactttcg ttataatatc tatcctcata tcatattagt
     4621 tttatattta cctgaatctg ttcatccttt ttttcctttc cctttgatgg atataaaatt
     4681 catacattta agcttcctac tgtattaact tttatagaag tgtatattat aattttatca
     4741 ctaaatagac aaaaaaatat cgatataaca tcaagaaata ttttactgag tttcgcttat
     4801 taaaagcaac tgaatttcta aagggtacag atgagctaat aagtgatatt gatataaaag
     4861 ttgtttttca ttaagtaatc tattttagta aatgttttaa agaaaaaacg ggatattctc
     4921 caaaagtgta cagaaatata aaaaaagaga tatgattttt tatttctttt gattatccaa
     4981 agagtaaata aaaaatattg taaaaaactt actaacaacg gcaatgcttt gaactatggt
     5041 aatactttga acttttgatt ggactttttt tgctaaaata atatatttaa atagcaaagt
     5101 aatctgttta atcaataaaa aataagagat aaaagaaatc aactaaaacc tttacctctt
     5161 attttgatag tttatcagag ctaatattta cactcttttt atttttgata cttcattaaa
     5221 aatgatgctt attctctttt catttttaat acttcattaa aattgatgct tactctctat
     5281 tcacttttaa tacttaatta gaactgatgc ttacactctg taaactcata aacatccatt
     5341 aatgcttcac caaatctttg atagtgaact acttccctct cacccaaaaa tctcaatata
     5401 tctttcaaat cagggtcatc agttaaattt attaattgat agtacgtagc caatgctttt
     5461 tgttcagcag ccatatcctc atgtaaatca gttaccgaat cgccaaagac attaatataa
     5521 cttgtagtaa agtttactcc attagaatct actggttgaa gaccatgtcc aaaattagca
     5581 taattactac caagtccagc agccttaagc tcttccggag tacaactgcc tattaattga
     5641 tataacactg aagaaattat ctcaacatgg gccatttcct ctgtacctat atcagttaaa
     5701 agtgcgcgag attttccagt tggcatagta tatctttgtt gaagatatct agttgcagct
     5761 gccaactcac cattaggtcc tcccaactga gtcatgatat attttgccat tctagggtct
     5821 gcttgtctta tgttaactgg atgttccagt gttttttgat aaatccacat attatcttac
     5881 tcctccttaa tatatattta ataataattc taattttaaa acttaataat tttatttata
     5941 aaatgatttg tcccatggcc aaggttcaga tatccatgac caaggatatt cacttgattc
     6001 atatccaaaa ttagttaatg ctccatattt agattcataa tcacacacag cttgtgtaaa
     6061 tttatcgcag tatctattgt ataaatcaag agctttctta tcccttggat tgttatctaa
     6121 atataaattt aattctatac aggcaaattg atattctgaa attctctcta aaagttctct
     6181 tctatttaat gacattagta catgttaccc ccttttttag ctctaactct atttttagga
     6241 tttgaatata gccaatcatt atagttattt gactctatta aatctaattc aggaaataaa
     6301 gtacctcttt ttaatgctgt ttttggattg tatgtgtcat cataaatttg acgattgatg
     6361 tatggtctag caaattcaca acaatagtct tgtttatcct cttttaaata tcccattaat
     6421 tatcctcctt aatttttaga attttaaaat agcattattt ttaaaaattg tatacttgtt
     6481 ctatattata gtcagtagtt ttaataggtc tttaataaga ttaaataa
//
LOCUS       DAMJWP010000198         6514 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.205, whole genome shotgun sequence.
ACCESSION   DAMJWP010000198 DAMJWP010000000
VERSION     DAMJWP010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6514
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.205"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            26..1768
                     /locus_tag="PC700_003027"
     CDS             26..1768
                     /locus_tag="PC700_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M3"
                     /protein_id="HDF2582302.1"
                     /translation="MVKSKKKIFVFLILIAVFIIFASYIRNYTNDTKKDSVRGTIPFE
                     KMEYKRPNIQSICENINKCNDKLLTAKTAKEQLNLFNEVDKIYQDFYSTLTIAKIRNN
                     IDSSDEFYSKEYKYLMSKSVDVDMSYKDFQDRFVTSKFSKELEKELGKDTFNYLKNIG
                     KLNSKEVEDLLKKEQDLVVKYEDLLAKSTVSIDGIEVDFEEALSRPNLSPEEYVKIYS
                     DYLKKYNPIFGNIFLELIQTRTEIASKQGFKNYIDYAYMNLNKDYSQEEAKKFRQDVK
                     DYIVPLYREISSKPSDSSIYIKVYKNRSFRKFDKVLEDISPKLKESFDYMKKYDLYDY
                     SSGKNKSPGGYTTYINKYKAPFLFNTWDNSFLGVTSFAHEFGHFYNYYNSINLNNKMQ
                     PSIDVCEVHSTSLEILFYKYFDDFFGKQSEAIKKEHLSIVLNTIIDACLYDEFQEIIY
                     KNPYMSLNDINKLFFDLEEEYGVSNNILREKNAPFWILVSHNFQVPFYYLSYGLASDV
                     SLQIWQLSQEDYRKAVDVYMDFLNQNTDAGFKDVVEKVNLQLPFQDGNLEEISSTLYD
                     YFGIDNPLELKNAS"
     gene            complement(1932..2126)
                     /locus_tag="PC700_003028"
     CDS             complement(1932..2126)
                     /locus_tag="PC700_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582303.1"
                     /translation="MKIITIGSSLITVLLFLSTMVCGFWIKNNKVTDASSIKFHMNSA
                     IFTGIFLLISTILLIIYIKK"
     gene            complement(2123..2704)
                     /locus_tag="PC700_003029"
     CDS             complement(2123..2704)
                     /locus_tag="PC700_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2582304.1"
                     /translation="MKSNDKDARERIIEVTLNLLNEVDDIEEITVRKIAERANVGVGL
                     INYHFKTKDNLLSTAIGDVMSNIIAELYDDSVYTLRPIEDLKNLLKKLCDTGLHYEKV
                     LPFVLNQCITNGDMQAELDIVPMLRKIFGNKKDEMSLRIIALQIILPIQISALSTESF
                     QLYSGINIKNKYERDKFIDILIENIIGEDVDVR"
     gene            2872..3690
                     /locus_tag="PC700_003030"
     CDS             2872..3690
                     /locus_tag="PC700_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2582305.1"
                     /translation="MFKIGDFSKLSKISIRMLRHYDEIGLLTPSHTNNTNGYRYYSAD
                     QLSTTNRIHALKDMGFGLYSIKEILTEYNDKESLIKYLNIHHSQVKEQLEDTQKKLLK
                     IETTIKRIGGNDIMKNYDVTIKNFAPKYMMTLRRVIPTYQDEGMLWHQAFLETKDQNV
                     QIEPPKYSKAVFYDTGYKEDSVDVEVQVAVSGKYKDTEHVKFKTVPSVTAATAIVNGN
                     FNQVADACEAIGNWISDNNYDVDGPMFNIYHVSPGNDSNPDNWVTEVCFPVKKK"
     gene            4415..5236
                     /locus_tag="PC700_003031"
     CDS             4415..5236
                     /locus_tag="PC700_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallohydrolase"
                     /protein_id="HDF2582306.1"
                     /translation="MKLKIVGSGGMSIIPSSFCKCKICEEARQKGGRYERLGPSLYID
                     DIKMLIDTPEDITVACNRQKISEVKHISISHHDPDHVKGIRIVEKIGCDFITGENNPI
                     GFYALPEVIEDINRTNLDCLKYYGDVINCISINETSHVKINNFDIDLINNNPDRNITF
                     YVIKENEKKVIYACCNPKPFTHSDMYFDADVLIISLVSDDGILNDGTKLEDTPFKDEI
                     FTLDEIVEIKNYYRIKKIIITHIDEMWGKSYGYYSELEKKLDNIYFAYDGMEIIV"
     gene            complement(5546..6325)
                     /locus_tag="PC700_003032"
     CDS             complement(5546..6325)
                     /locus_tag="PC700_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HDF2582307.1"
                     /translation="MNYKKIAIITGANSGFGKEFLKLLINEEEITEIWAVARNKARLN
                     QLVDEFGSKVKVFSKDLSKIEEVKEIGTFLSKENVCIKYLINNAGFAKFCSYNDLSID
                     ESINMIDLNISAVVVLGLICIPYMQKGSHIMNISSQASFQPLPYQNIYSSTKAFIRNY
                     TRALNVELKSKGINAIAVCPGWMSTNLFKRGIVVAEKGTKNFPGIVSPDVVAKKALKD
                     AKKNKDISVYGINTKSSHLLAKLLPQKMMMKVWLMQQHIKE"
BASE COUNT         2446 a          883 c          834 g         2351 t
ORIGIN      
        1 ctatatccat gattggagga tttttatggt taaatccaag aagaaaatat ttgtttttct
       61 aattttaatt gcagttttta ttatatttgc atcatacata agaaattata ctaatgatac
      121 aaaaaaagat tctgttagag gaactattcc ttttgaaaaa atggaatata agcgtcctaa
      181 tatacagtca atttgcgaaa acattaacaa gtgtaacgac aaacttttaa cagctaaaac
      241 agctaaggaa caattaaatt tatttaatga agttgataaa atttatcagg atttttacag
      301 tacacttact atagcaaaaa ttagaaataa catagattca tcagacgaat tttactctaa
      361 ggaatataag tatcttatga gtaaatcagt agatgttgac atgtcataca aagactttca
      421 agatagattt gtaacttcta aattctctaa agaattagaa aaagaacttg gaaaggatac
      481 atttaattat ctaaagaaca ttggaaaact taattctaaa gaagttgaag atttacttaa
      541 aaaagagcaa gacctagttg taaaatatga agacttatta gctaaatcta cagtatcaat
      601 agatggtata gaggtagact ttgaagaagc cttgtcaaga ccaaatctta gccctgaaga
      661 atatgtgaaa atttattcag attacttaaa aaaatacaat cctatatttg gtaacatatt
      721 cttagaactt atacagacaa gaactgaaat agcaagtaag caaggattta agaattatat
      781 agattatgcc tatatgaacc taaacaaaga ctattctcaa gaagaagcta aaaaatttag
      841 acaagatgta aaagattaca tagttccatt atatagagag atttcatcta aaccatctga
      901 ttcttctatt tatattaaag tatataaaaa tcgctcattt aggaagtttg ataaagtact
      961 tgaagatata tctccaaaac taaaagagag cttcgactat atgaaaaaat atgacttgta
     1021 cgattattct tctggaaaaa acaaatcgcc tggaggatat actacttata taaataaata
     1081 taaagcacct ttcttgttta atacatggga caattctttt ttaggtgtta ctagttttgc
     1141 tcatgagttt ggtcattttt ataattacta caacagtatt aacttaaata ataagatgca
     1201 accaagtata gatgtatgtg aagttcattc aacatcttta gaaattctat tttataaata
     1261 ttttgatgat ttttttggta aacaatctga agctataaaa aaagaacact tatcgattgt
     1321 tttaaatact attattgatg cttgtcttta tgatgaattt caagaaatta tatacaagaa
     1381 tccatatatg agtctaaatg atatcaataa attgtttttt gatttagagg aagaatatgg
     1441 agtttccaat aatatactta gagaaaaaaa tgctcctttt tggatacttg tatctcataa
     1501 cttccaagtt cctttttatt acttgagtta tggtcttgca tctgatgtat cacttcagat
     1561 atggcaatta tcacaagaag attacagaaa agctgttgat gtatatatgg attttttaaa
     1621 tcaaaatact gatgctggat ttaaagatgt ggtagaaaag gtaaatttac aattaccttt
     1681 ccaagatgga aatttagagg aaataagctc tactttatat gattattttg gtatagataa
     1741 tccacttgaa ctaaaaaatg cttcttaatt tctaatactt tatactcact cttaagattt
     1801 agtattagat tgaaataagt gatatttttt cacacacaat taatgtttaa taaaattatg
     1861 tggtaaagta tcagaaattt ttaagactct tgtctttata atttctgata ctttatttat
     1921 taataatatt attatttttt tatatatata attaaaagga ttgtggaaat caataaaaat
     1981 atccctgtaa aaatagcact attcatatgg aacttaatag aacttgcatc tgttacctta
     2041 ttattcttaa tccagaaccc acaaaccata gttgaaagaa aaagtaaaac agtgataaga
     2101 cttgaaccaa tagttataat tttcatctta catcaacatc ctctccaata atattttcta
     2161 ttaaaatatc aatgaattta tctcgctcat atttattctt tatatttata ccactgtata
     2221 actgaaatga ttctgtagat aaagcagaaa tctgtattgg taaaataatt tgcagggcaa
     2281 tgattcttag actcatttca tcttttttat ttccaaaaat tttacgaagc atagggacaa
     2341 tatcaagttc tgcttgcata tctccattgg ttatacattg attcaacaca aaaggaagca
     2401 ctttttcata atgtaatcca gtatcacaca atttttttaa taaatttttc aaatcttcaa
     2461 taggtcttag agtatataca ctatcgtcat atagctctgc tattatattt gacatcacat
     2521 ctcctatggc agtactcaac aaattatcct ttgttttaaa atgataattt attaaaccaa
     2581 ctccaacatt tgcacgctca gcaatttttc taactgtaat ctcctcaata tcatctactt
     2641 catttaaaag attcaatgta acttcaatta tacgttctct agcatcttta tcattacttt
     2701 tcataataga cctccaaaat aattaacatt ttaggttctg aacatgttca gtataatatt
     2761 tttttcatca tatgtcaata taatttaatt ataattttag ttgactctaa cattatgata
     2821 tggtttatat taatattaag ctaaaagtac ctttataaag gagacaaaaa catgtttaag
     2881 ataggcgatt tttcaaagct aagtaaaatt agtattcgta tgttacgtca ttatgatgaa
     2941 ataggacttt taactccttc tcatacgaat aacactaatg gatatagata ctatagtgca
     3001 gaccaacttt ctactacaaa tagaatccat gcactaaaag atatgggctt tggcttgtat
     3061 tctataaaag aaattttgac agaatataac gataaagaaa gtcttataaa atatctaaat
     3121 atccaccatt ctcaagtaaa agaacaatta gaagatacac agaaaaaact attaaaaata
     3181 gaaaccacta ttaaaagaat tggaggaaat gatattatga aaaattatga tgtaactata
     3241 aagaattttg caccaaaata tatgatgaca cttagaaggg ttataccaac ttatcaagac
     3301 gaaggaatgt tatggcatca agcattttta gagacaaaag accagaatgt tcaaattgaa
     3361 cccccaaagt attctaaagc agtcttttat gatactggat ataaagaaga ttctgtagat
     3421 gttgaagtac aagttgctgt atctggaaag tataaagata ctgaacatgt taaatttaag
     3481 actgttccaa gtgtgactgc tgcaactgca attgttaatg gaaatttcaa tcaagttgca
     3541 gacgcttgtg aggctattgg taattggata tcagataata actatgatgt ggatggtcct
     3601 atgtttaata tttatcatgt aagtcctggc aacgatagta atcctgataa ttgggtaaca
     3661 gaggtatgct tcccagttaa aaagaagtaa tttttatatt aattggataa tattttaatt
     3721 tgttaaacaa attactttta gtattatgta taaataccta caatttaatt aggatattat
     3781 cttaatttgt taaacaaatt acttttagtg ttatgtataa atatctacaa tttaattagg
     3841 atattatctt aatttgtcag ataaatcact tgtgatgtta tgttcgaatg actagaattt
     3901 gattaggata ttatcttaat ttgtcagata aattacttat aagattatat acaaccctta
     3961 tactctagtt aaatatttga atcactaata taaattagtt tttaacaaaa caaagagtaa
     4021 agtacaaata attcctttgt actctactct ttttaacttt ctttgtataa taatacaatg
     4081 ataaatataa aaaattatat attccattct atagaaatat gttcaccaat aacatatatg
     4141 ttattaatca atattttata tcaaaattac atgaacctta tagaataatc atatcttatt
     4201 acaagaaaaa tgtcttatct atattgaata aagtcattcg gctcatatac aaagtcaatt
     4261 tcattgagaa atatttaata taatcttata aaaatttcat actattcttt catatttaca
     4321 tgtgatataa tttattaact cataattttg ttaatcagtt gtgattgtag attatttata
     4381 taatctaata tttttagaag gtggtgtaat aactttgaaa ttaaaaattg ttggtagtgg
     4441 cggtatgtca attattccaa gttcattttg taaatgcaaa atatgtgaag aagccaggca
     4501 aaaaggcgga agatatgaac gattaggacc aagtttatat attgatgata taaaaatgct
     4561 gatagacaca cctgaagata ttacagtagc ttgtaacaga caaaaaatat cggaagtaaa
     4621 acatatttca atttctcacc atgacccaga ccatgttaag ggtattagaa ttgtcgaaaa
     4681 aataggatgt gactttatca caggagaaaa taatccaatt ggcttttatg ctcttcctga
     4741 agtaattgaa gatataaacc gaacgaatct cgattgtctt aaatattatg gtgatgttat
     4801 aaattgcatt tctattaatg aaacttcgca tgtaaagata aataattttg atattgattt
     4861 gattaacaat aacccagaca ggaatataac cttctatgtt atcaaagaga atgaaaaaaa
     4921 agtcatatat gcctgctgta atcccaagcc atttactcat agtgatatgt actttgatgc
     4981 tgatgtttta ataataagtc tagtttctga tgatggtata ttaaatgatg gtacaaaact
     5041 tgaagacaca ccatttaaag atgaaatatt tactttagat gagattgttg aaattaaaaa
     5101 ctattataga attaaaaaga ttatcattac acatattgac gaaatgtggg gaaaatcata
     5161 tggttattac agtgaattag aaaagaaact tgataacatt tattttgcat atgatggtat
     5221 ggaaattata gtctaaatca ttaagtaata agcataatgc aaaataagaa aacttcaatt
     5281 taacagtata gttaaactat gtaatatcaa catatctcag tttttacaaa atatcatagt
     5341 atttatcttt ataaaatttc aagtcctaca tattttaata cttcaatatc taaataaagt
     5401 aaaatcaaaa atatattttt tatacattat agacggctta tcggcactag ataagccgtt
     5461 ttgtcacaat tattatttgc tcaacaaaac ttcttataat atataataga ttgaatctcc
     5521 aactgatact taattaccga aactcttatt ccttaatatg ttgttgcatt aaccaaacct
     5581 tcatcatcat tttttgtggc aatagttttg ctaataaatg actggatttt gtatttatac
     5641 catacacaga aatatcctta tttttctttg catccttcaa tgcttttttg gcaacaacgt
     5701 ctggagacac tatgcctggg aaattttttg tccctttttc agctacaact atacctctct
     5761 taaatagatt tgtactcatc caacctggac atacagcaat tgcatttatc cctttactct
     5821 taagttcaac atttaatgct cttgtataat ttcttataaa cgctttagtt gaactataaa
     5881 tattttgata tggcaatggc tggaatgatg cttgtgaaga aatattcata atatggcttc
     5941 ccttttgcat atatggaata catataagtc ccaatactac aacagcacta atatttaaat
     6001 caatcatatt aatagattca tctatactta aatcattgta tgaacagaat tttgcaaaac
     6061 cagcattgtt tataaggtat tttatacata cattttcttt acttaaaaat gtaccaattt
     6121 cttttacttc ttctattttt gataaatctt ttgaaaaaac ttttacctta cttccaaatt
     6181 catctactaa ttgatttaat ctcgccttgt ttcttgcaac tgcccatatt tctgttattt
     6241 cttcttcatt tattaataac tttagaaact ctttaccaaa accactatta gcacccgtta
     6301 taattgcaat cttcttataa ttcataatta ttctccttta ccataagttt atacgctcaa
     6361 actgtaaact tattttttac aaataaaaca ccctctattc agatgttata ttcatatcat
     6421 ttgagatatt atatttatat cattttcaat aattgttata tagcataaaa aatagtgtta
     6481 atattataga atcagtattg attatattta aaaa
//
LOCUS       DAMJWP010000199         6507 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.206, whole genome shotgun sequence.
ACCESSION   DAMJWP010000199 DAMJWP010000000
VERSION     DAMJWP010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6507
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.206"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     misc_binding    105..316
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            479..1768
                     /locus_tag="PC700_003033"
     CDS             479..1768
                     /locus_tag="PC700_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HDF2582308.1"
                     /translation="MEEEKINYALEQIPENQKRGWVAMFSVLVAIGVDLSSVILGAEL
                     AQSMPMKQAILSVIVGSFFSAILYTTCSLVGSSTSLSTSMITKYVFGEAGAKIFSLVI
                     GVSLLGWFGVQVGFFAQNAQIIIKDIFNLDVSMQILSLIGGLLMMSTAIYGYKAMEKL
                     SVYSVPFLLVLMMLTIFLAFRANGISVDDNMKSTMTFAGGVSLSMSIIIVGAIVSPDI
                     SRWAKSRRDCALSSFLGIQFGNAFMIIVSIVLVKCMGTSDIMRIFITLGIAIPGIIVL
                     TLAQWTTNTSNVYSASLSIALVLKKAPEKVLTIVLGIIATLLAVFGIYEGFIGFLNLL
                     GIVIAPVGGVYTAEYYIVKQELKGFDKGVLYKPIVKRSIVSWIIGILITYLSTYGFIT
                     LTTIAPLDGFIAGFVVQSIIGKVLCSTKNKQEIDKAV"
     gene            1822..2901
                     /locus_tag="PC700_003034"
     CDS             1822..2901
                     /locus_tag="PC700_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HDF2582309.1"
                     /translation="MRFLTEESVDKIAVGAAVLGTGGGGDPYVGKLVAKQAIKKYGPV
                     KVISLDELDDDALVVPVSGMGSPVITIEKLLSEVELTTPLEIMEKLLNRKVDVIIPIE
                     IGGINSLMPIAVAAKKGLPILDADSMGRAFPEAQMVTFYLEGYEASPAVMSDEKGNSA
                     ILYPVDGIWSERLARTLTVEMGGSSSISDYNLSGAQVKKAAIGNTLTIAETIGGFLLE
                     NKSDSKGAVENILKELCGYKLFEGKVIDIKRELKGGFTRGHAFFAGINEYDGEYSILF
                     QNENLIAKKGDTPLCITPDLIAVLDLETGFPITTERIKYGSRVMVVAFPCNEKWRTEK
                     GIETVGPGYFGYDVEYKTVEELQGK"
     gene            2922..4466
                     /locus_tag="PC700_003035"
     CDS             2922..4466
                     /locus_tag="PC700_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HDF2582310.1"
                     /translation="MYRVGIDVGGTNTDACILDGELKVIHSVKVATTKDVETGVYNAL
                     KKVIDESKIDHSLIKYAMLGTTHCTNAIVERKRLNNVAMIRIGKPATTAILPFIDWPE
                     DLREKIELDSILVSGGYEFDGRKINELCKDEVVEFCNRIKGKVESVAISGVFAPVNKQ
                     QEEEVAKWVREILGDIPISLSNEIGNIGILERENGAILNSALSQVGKAVSLGFEKALK
                     DLGIDAQIYFSQNDGTLMNLEYTMKYPIFTIGCGITNSIRGASFLSKVKNAIVLDVGG
                     TTSDLGVLYNGFPRESSIPVTIGGVHTNFRMPDVLTIGLAGGTCVKGEKDNIKIGPES
                     VGYRITEDAIIFGGDTLTLSDVVTKLDMAFEDRKSNVENLDKEYCNEVYKKVILKLED
                     AVDQMKTSQDDVELVLTGGGSIIVPEKLKGISNVIRPPHYTAANAIGAALGQISGDVE
                     KVYSLDKMSREDAIEDAKKEAVNKALKAGAKEDTIEVLNIEDVPLAYLPGNALLIKVK
                     VVGDLI"
     gene            complement(4629..5171)
                     /locus_tag="PC700_003036"
     CDS             complement(4629..5171)
                     /locus_tag="PC700_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HDF2582311.1"
                     /translation="MKSYIALLRGINISGKNKIIMSELKASFVELGYSEVSTYLNSGN
                     VVFLSDVDDNKVISNTIRLMIKKQFELEIPVFIISQEELKDILNNAPNWWGDNNKEIY
                     DNLIFMFPDLSYDEFYDEVGNPKEEYEKVYHYKNAIFWSFSRKDYKKTNWWSKTASSN
                     VSDKITIRTANTVRKIVGMR"
     gene            complement(5319..5603)
                     /locus_tag="PC700_003037"
     CDS             complement(5319..5603)
                     /locus_tag="PC700_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2582312.1"
                     /translation="MAKNTKQTTVHIDETLLKEVKRIGINENKSISQIINESIIDYIL
                     VYRENEDKEKNEHDKNLNETINEFKKEEEKRMQAKSAARAIAEAKKRVLY"
     gene            complement(5666..6430)
                     /locus_tag="PC700_003038"
     CDS             complement(5666..6430)
                     /locus_tag="PC700_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDF2582313.1"
                     /translation="MNNYLKFATYQLIGFTSSIPSLIKIKKNPDKFSLKEKFEFMQKQ
                     AKKSLDIVNIELNIIGKETLPKEPLLFVVNHSSMLDSFILTASVERPIGCVIADEPVW
                     RNIPIFKEWAKLLRCVYVNRKNNREGIKSIAQASQNILTGQSMAVFPEGDLTWIKEPN
                     SLVSEFRSGALKIAYKAKCPIVPLVIKNSKDTYEGYQPIGKINSVPVEVEFLEPIYDH
                     IENPRLKSSVLGENIKNKMINTIENFRKSNKTFKEF"
BASE COUNT         2263 a          805 c         1130 g         2309 t
ORIGIN      
        1 atttacatat aaaactaaaa aatgtaagta attgactttt taagttgaca aaatattgca
       61 tatatattat gatataaatc ataaattaac tacaaatcaa tattatatat tcgtagaaga
      121 agaaagtagc ataaaggaat tttacagaga gttagatttg gctgagaact aatattggaa
      181 tttatgtgaa aaggactttg gagaatacta ttgtaattat gttagaatag tggtttaatt
      241 cgccttaaga aaatgagtga gtaaaattaa tttttactaa taagagtggt aacacggatt
      301 tatattcgtc tcttggattt ttccaagagg cgttttttat tataaactta tttcagaaaa
      361 tttaaaacta tatattaaat gaaaattata aaaatatgat agttaataaa tttataaatt
      421 agaaaattgt aaaatatgct aaggtcagta gttttacgat ttaaatggag ggatgaaaat
      481 ggaagaagaa aaaattaact atgctctgga acagattcca gagaatcaaa aacgtggttg
      541 ggtagctatg ttttcagttt tagtagcaat aggtgtggat ttatcatctg ttattttagg
      601 cgctgagttg gcacagagta tgccaatgaa acaagcaata ttgtcagtaa ttgttggttc
      661 atttttttct gcaattttgt atactacatg ctctttggtt ggttcatcta caagtttatc
      721 tacatctatg ataacaaagt atgtatttgg agaagctgga gcaaaaatat tttctctagt
      781 tataggggtt tctctattag gttggtttgg agttcaagta ggtttctttg cacaaaatgc
      841 acagataata ataaaagata tatttaattt agatgtcagt atgcagattt taagcctaat
      901 aggtggtctc ttgatgatga gtactgcaat ttatggatat aaggccatgg agaagttgag
      961 tgtgtattca gtaccatttt tattagttct tatgatgcta actatattct tagcttttag
     1021 agcaaatgga atttcagttg atgataatat gaaatctact atgacatttg caggtggagt
     1081 ttctctgtca atgagtataa taatagttgg tgcaattgta tctcctgata taagtcgttg
     1141 ggctaaaagt agaagagact gtgctctttc aagctttttg ggaatacaat ttggaaatgc
     1201 ttttatgata atagtttcga tagtattagt aaaatgtatg ggaacttcag atattatgag
     1261 aatttttatt acattaggaa ttgctatacc aggtataata gtacttacat tagcacaatg
     1321 gactacaaat acaagtaatg tatattcagc atcattaagt atagctcttg tacttaagaa
     1381 ggctcctgag aaagtattaa ctatagtcct tggtattata gcaacattat tggctgtatt
     1441 tggaatatat gaaggtttta taggattttt aaatttactt ggaattgtaa ttgcacctgt
     1501 tggtggtgta tatacagcag aatattacat agtaaagcaa gaacttaaag gctttgataa
     1561 aggagtatta tataaaccaa tagttaaacg ttcaatagtg tcttggataa ttggtatatt
     1621 aataacttac ctttcaacat atggatttat aactcttact actatagcac ctttagatgg
     1681 ttttatagca gggtttgtag tacaatctat tattggaaaa gtgctttgtt caacaaaaaa
     1741 taagcaagaa atagacaaag cagtataaag aaatttgttt ataaagtata aaataaatta
     1801 gaaatatgag gagaaagtaa catgagattt ttaacagaag aaagtgtaga taaaatagca
     1861 gttggtgcag cagtgcttgg aactggaggt ggaggtgacc catatgtagg taaattggtt
     1921 gcaaaacaag ccattaaaaa gtatggtcct gtaaaagtaa taagtttaga tgaacttgat
     1981 gatgatgctt tggtagttcc agtatctggt atgggttctc ctgtaattac aatagaaaaa
     2041 ttactttcag aagtagagtt aactacccct ttagaaataa tggagaaatt attaaataga
     2101 aaagtggatg ttataattcc tatagaaata ggagggataa actcacttat gcctatagca
     2161 gttgctgcta aaaaaggact tcctatctta gatgcagatt ctatgggtag agctttccct
     2221 gaagctcaaa tggtaacttt ttatcttgaa ggatatgagg catctcctgc cgtaatgtct
     2281 gatgaaaaag gtaattctgc catcctttat cctgtggatg gaatttggtc agaaagatta
     2341 gctagaactt taactgtaga aatgggagga agttcttcta ttagtgacta taatttaagt
     2401 ggagctcaag ttaaaaaagc tgctataggt aatactctta caatagctga aactatagga
     2461 gggtttttac ttgaaaataa atctgattca aaaggtgctg ttgagaatat actaaaagaa
     2521 ttgtgtggat acaagttatt tgaaggaaaa gtaattgata ttaagagaga gttgaaaggt
     2581 ggatttacta gaggacatgc cttttttgct gggattaatg agtatgatgg agagtattct
     2641 atattattcc aaaatgagaa tttaattgcc aaaaaaggtg atacaccact ttgtattaca
     2701 ccagatttaa tagcagtatt agatttagaa actggatttc caataactac agagagaata
     2761 aaatatggct ctcgtgttat ggtagtagct tttccttgta atgaaaagtg gagaacagaa
     2821 aaaggaattg agacagttgg acctggatac tttggatatg atgtggaata taaaacagta
     2881 gaagaattac aaggaaaata aaagtttagg aggaaatttt gatgtatcgt gtaggaattg
     2941 acgttggagg aacaaataca gatgcttgta tactggatgg ggaattaaaa gtaattcatt
     3001 cagttaaggt ggcaactaca aaagatgttg aaactggtgt ttacaatgcc ttaaaaaaag
     3061 ttatagatga aagtaagatt gaccattcat taataaaata tgctatgctt ggtacaactc
     3121 attgtacaaa tgctattgta gaaagaaaaa gacttaataa tgtagctatg ataagaatag
     3181 gaaagccggc cactactgcc attcttccat ttatagattg gccagaagac ttaagagaaa
     3241 aaatagaatt agattcaata ttggtatctg gtggatatga gtttgatggt agaaagataa
     3301 atgaactttg taaagatgaa gttgtagagt tttgcaatag aataaaaggc aaagtggaaa
     3361 gtgttgcaat aagtggagta tttgctccag taaataaaca acaagaagaa gaagttgcaa
     3421 aatgggttag agaaatttta ggggatattc ctatatcttt atcaaatgaa ataggtaata
     3481 taggtatatt agaaagagaa aatggagcta ttttaaattc tgcattgtct caagtaggta
     3541 aagctgtatc tttaggattt gaaaaagcac ttaaagactt aggtatagat gctcaaatat
     3601 atttcagtca aaatgatgga acattaatga atttagagta tacaatgaaa tacccaattt
     3661 ttacaattgg atgtggaata acaaattcta taagaggagc ttccttctta tcaaaagtaa
     3721 aaaatgcaat agtattagat gttggtggaa ctacaagcga cttgggtgta ctatataatg
     3781 gattccctag agaatcatct attccagtta caattggagg agtacataca aactttagaa
     3841 tgccagatgt tttaactatt ggtcttgctg gtggtacatg tgtaaaaggc gaaaaagata
     3901 atataaagat aggtcctgaa agtgtgggat atagaataac tgaagatgca ataatatttg
     3961 gtggagacac tctaacttta tcagatgttg ttacaaaatt agatatggcg tttgaagata
     4021 gaaaaagcaa tgttgaaaac ttagataaag agtattgtaa tgaggtatat aagaaggtta
     4081 ttttaaaatt agaagatgct gtagaccaaa tgaagactag tcaagatgat gttgaattgg
     4141 ttttaactgg tggaggaagt ataattgttc ctgaaaaact aaaaggcata agcaatgtta
     4201 taagacctcc tcattatact gctgcaaatg ctattggtgc agctttagga caaataagtg
     4261 gagatgtaga aaaagtatac agtttagata aaatgtcgag agaagatgcc atagaagatg
     4321 caaaaaaaga ggctgtaaat aaagctctaa aagctggtgc taaagaagat actatagaag
     4381 tattaaatat agaagatgtt ccacttgcat accttcctgg taatgcactt cttataaaag
     4441 taaaagttgt tggtgattta atttaaagaa taaatataag tatttccaga attatataaa
     4501 tatttctgga ctcattttta aacttatttt atcaataaaa acagcataac ttttgactat
     4561 attcttataa gtttccgaac agccgatata tacctatttt ttgaggttta tcggctgttt
     4621 gttatctttt atcgcattcc aactattttt cttaccgtat ttgctgtacg aattgtaatc
     4681 ttatcactta catttgaact ggcagtcttt gaccaccaat ttgtcttttt ataatcttta
     4741 cgactaaaag accaaaagat agcattttta taatgatata ctttttcata ctcttcttta
     4801 ggatttccaa cctcatcata aaactcgtca taagataagt caggaaacat aaaaataaga
     4861 ttatcatata tttctttgtt gttatctccc caccaattag gagcattatt tagtatatct
     4921 tttaactcct cttgtgaaat aataaaaact ggtatttcca attcaaactg tttttttatc
     4981 attaatctta ttgtatttga gataaccttg ttgtcatcta catcacttaa aaagacaaca
     5041 ttaccactat taaggtatgt agaaacttct gaataaccaa gttctacaaa gcttgctttt
     5101 aattcagaca taattatctt gttcttacca cttatattga tacctcttaa taatgcgata
     5161 taacttttca tataataagt tcacctctaa acattcacat ttttctatgt agtacaaact
     5221 ggaatttgca tcttttataa ttctttcaaa acttcttcat atgtaatttt tttaatataa
     5281 aaaaatcggt gcttaatttg taccgatttt tatttttctt agtaaagaac tcgttttttt
     5341 gcttctgcta tagctctagc agcacttttt gcttgcattc ttttttcttc ttctttttta
     5401 aattcattta ttgtttcatt tagattctta tcatgttcat ttttttcttt atcttcgttt
     5461 tctctgtata ctaatatata atctattatt gattcgttta ttatctgaga tatactttta
     5521 ttttcattaa ttcctattct cttaacttcc ttaagtagag tctcatcaat atgaaccgtt
     5581 gtttgctttg tattttttgc caccttaatc acctcttaaa aaaaattcta acattaaaat
     5641 tttttttagt caataaattt taatattaaa attctttaaa tgttttattt gattttctaa
     5701 aattttctat tgtatttatc atcttatttt ttatattttc acctagtaca gaagacttta
     5761 gtctaggatt ttctatatgg tcataaattg gttctaaaaa ttcgacttcg acaggaacag
     5821 agttaatttt accaataggt tggtatcctt cataagtatc ttttgaattt tttattacga
     5881 gtggaacaat agggcatttt gctttatatg ctatctttaa agctccactt ctaaactccg
     5941 aaaccaatga atttggttct ttaatccatg ttaaatctcc ttctggaaac actgccatac
     6001 tttgacctgt aagtatattt tgagaggctt gggctatact ctttataccc tcacgattgt
     6061 tttttctgtt tacatataca catcttagta attttgccca ttctttaaat ataggtatat
     6121 ttctccaaac tggttcatct gcaattacac atccaattgg tctttctaca cttgctgtta
     6181 gtatgaagct gtcaagcata ctagaatgat ttacaacaaa tagaagtggt tcttttggca
     6241 gtgtttcctt accaattata ttaagctcta tgttcactat atctaaagat tttttagctt
     6301 gtttttgcat aaattcaaat ttttccttta agctaaattt atcaggattc tttttaattt
     6361 taataaggct aggtatagaa gatgtaaaac ctataagttg atatgtagca aattttaagt
     6421 aattattcat aaaattatct ccttaaaata aaattattta ttactgtaaa gattttaaca
     6481 taaaataaaa tttaagtcta ttttaat
//
LOCUS       DAMJWP010000200         6354 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.207, whole genome shotgun sequence.
ACCESSION   DAMJWP010000200 DAMJWP010000000
VERSION     DAMJWP010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6354
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.207"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            233..1177
                     /locus_tag="PC700_003039"
     CDS             233..1177
                     /locus_tag="PC700_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HDF2582314.1"
                     /translation="MKFLRIEDEVIYSKKRYKLKREIDKLFLDEEYFQIEPQLFEEYD
                     EFTTINNKIPEESMVKVVNGKVMVLRADITTSIIKSLVPRWEDGLKLKLFYNSSIYKN
                     KNTVGIKEIRQIGCEYLGEASVEADREVVKLALKILEKYNNNFILEVGSSKYIHGLLE
                     ELNLNKNCENQIKNLLYTKNTHELKVYIEELKIKREVKELLSNILSLQGNLYNVIEKS
                     NKFYCNNKMKQALEELKQVNNLIEECNFLDKARFDLSMITMLDYYEGIMFRGYYPNSY
                     KEILSGGRYDSLTKEYGKEIPAIGFTLSVDELMKYVYK"
     gene            1199..1825
                     /locus_tag="PC700_003040"
     CDS             1199..1825
                     /locus_tag="PC700_003040"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HDF2582315.1"
                     /translation="MDYLTIALAKGRIEGESFKKFKKMGLGDSIDTDTRKLIFKDEEN
                     KIIYIHVKPSDVVTYVEKGVADLGIAGKDTILENETDVYEIYDLGFGKCKFAVAGLKG
                     DSIYREDEYLKVATKYPNIAKKYFKEKGQKIEIIKLNGSVELAPIVGLSDVIVDIVET
                     GNTLKANGLEILEDICNISARIISNRASYRFKYEQIQNIIRLFEELDN"
     gene            1859..2908
                     /gene="hisC"
                     /locus_tag="PC700_003041"
     CDS             1859..2908
                     /gene="hisC"
                     /locus_tag="PC700_003041"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDF2582316.1"
                     /translation="MREKESIRELRGYEPNHVNCKVKLDANEGSKRLFKYLIKEISDS
                     DIDLNLYPEDSYSDLKESIIDYINISGVNKKNLLVGNGSSEIIDLIIHTFVDKDEVIL
                     SFSPSFSMYSIYSQINGSKFIGVESDENLVINIDNVIEKVKENNPKIVIVCNPNNPTG
                     TILKREEIIKLLDSTNSLVVLDEAYMDFGEESMLSDVFKYDNLIVLRTLSKAFGLAGI
                     RTGYMLSNSSLINSVEKVRPPYNLNSLSDFIATRALRNKDVVKAYIKEVKEEREVLYE
                     EMIGMGIKAYKSQANFILFYSEIENLSQKLIDRGVLIRKFGGKLENYYRVTIGDKEEN
                     SMFVGAIRDILKKEK"
     gene            2911..3489
                     /gene="hisB"
                     /locus_tag="PC700_003042"
     CDS             2911..3489
                     /gene="hisB"
                     /locus_tag="PC700_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HDF2582317.1"
                     /translation="MRIWKVERNTFETQILVELNIDGSGKAEIDTGIGFLDHMLTLMS
                     FHGKFDLKVICKGDTYVDDHHSVEDIGIAIGEAFKNALGDKKGIRRYSNIYIPMDESL
                     SMVAIDISNRPYLVFNAKFDTQMIGSMSTQCFKEFFRAFVNESRVTLHINLLYGENDH
                     HKIESIFKAFARALKEGSEIVSNEIASSKGVL"
     gene            3491..4096
                     /gene="hisH"
                     /locus_tag="PC700_003043"
     CDS             3491..4096
                     /gene="hisH"
                     /locus_tag="PC700_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HDF2582318.1"
                     /translation="MNIIVDYGLGNIDSVSRGFRKAGIETKISSDIDEIKQADSLILP
                     GVGAFRDSISALDKLGLIPIIKEHVSKGKFMIGICLGMQLLYEKSYEYGEYEGLGLIK
                     GSIDKLDITLKVPHMGWNNLKFNKANDDILKYINEDDYVYFVHSYYANSSNEELVAFS
                     EYEKKIPAIVRKGNVYGIQFHPEKSGEVGLNILRAYGEMIK"
     gene            4093..4815
                     /gene="hisA"
                     /locus_tag="PC700_003044"
     CDS             4093..4815
                     /gene="hisA"
                     /locus_tag="PC700_003044"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HDF2582319.1"
                     /translation="MIIFPAIDIKDNKCVRLTQGEFDKVNVYYDNPLEVAYKWKNEGA
                     EYIHIVDLNGARSEFGINTKIIEDIANNIDIPIQVGGGVRDKGKVKSLINAGVTRVIL
                     GSIAIENLNLVEELVNEYKEKIVVSIDAKDGKVAVRGWEVVSDVDSLTLCKQLEKIGV
                     QTIVYTDISKDGMLQGPNFDIYERIAKETSLNVIASGGVTSIEDVKRLKAMNLYGAII
                     GKALYDKKIDFKEAQQLCLLGE"
     gene            4797..5555
                     /gene="hisF"
                     /locus_tag="PC700_003045"
     CDS             4797..5555
                     /gene="hisF"
                     /locus_tag="PC700_003045"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HDF2582320.1"
                     /translation="MLTRRIIPCLDVRNGRVVKGKKFKDIVDVDSPEVLGKFYSDCGA
                     DELVFYDITASNEERKTSLEFVTKVAENINIPFCVGGGVNKLEDFTDILRKGADKVSI
                     NSSAVKNPELIREASLKFGAQCVVLSIDAKKNEEGSWSVYVKGGREKTNLDAIEWAVK
                     GVELGAGEIVVNSMDEDGMKNGYDIELLSKITSLVNVPVIASGGAGKKEDFYEAVNKS
                     NVDGILAASVFHFGEIKINDLKKYLKNMGVEVRL"
     gene            5583..6251
                     /locus_tag="PC700_003046"
     CDS             5583..6251
                     /locus_tag="PC700_003046"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HDF2582321.1"
                     /translation="MDNKCNNVYSDEVEEFIRSIKFDDKGLVPVVVQEVVSKDVLMLA
                     YMNKDAIKKTLKDKVACYFSRSRQKLWVKGETSGNTQKVVKMSYDCDVDTILLFVEQT
                     GVACHTGNYSCFYRDLFDDSSKMEPEVQTNILKELYDLINERKNNPVEGSYTNYLFEK
                     GIDKILKKVGEESSEVIIASKNTDKSELIYEISDLVYHTLVLMIEKGVEIDEIKKELL
                     KRRK"
BASE COUNT         2589 a          539 c         1254 g         1972 t
ORIGIN      
        1 tagtaaaatt aaatacaaaa ttaaatcatc accatcatca tagattaaag ggattgtaat
       61 atgaaattaa ttcatagaca caagccctgt ttttagtgta attggtcttg tatctatgat
      121 gggaagtaat tttaggagaa aattatagcc atataggtat aaagcctata tggctttttt
      181 attgttttaa atttgcaaat ataagagtaa atttaatgaa gagggtgaga atgtgaaatt
      241 tttgaggatt gaagatgaag ttatttattc taaaaaaaga tataaattaa aaagggaaat
      301 agataaactt tttttggatg aagaatattt tcaaatagaa ccacaattat ttgaagaata
      361 tgatgaattt acaactataa ataataaaat tccagaagaa tctatggtaa aggttgtaaa
      421 tggaaaagta atggtactta gagctgatat aactacaagt ataataaaaa gccttgtacc
      481 aagatgggaa gatggtttaa agcttaagct tttttataat tcatcaatat ataaaaataa
      541 aaatacagtt ggaataaaag agataagaca aataggttgc gagtatctag gagaagcctc
      601 tgtagaagca gatagagaag tagtgaaatt agctcttaag atacttgaaa aatacaataa
      661 taattttatt ttagaggttg ggagtagcaa gtatattcat ggtttgttag aagaattaaa
      721 tttaaataag aattgtgaaa atcaaattaa aaatttacta tatacgaaga acactcatga
      781 attaaaagta tatattgaag aactgaaaat taagcgtgaa gtaaaagagc tattatcaaa
      841 tattttaagc ttgcaaggga atttatacaa cgttattgaa aaatcaaaca agttctattg
      901 taataataag atgaagcaag ctcttgaaga attaaagcaa gtaaataatt taattgagga
      961 atgtaatttt ttagataagg ctagatttga tttatcaatg attactatgc ttgactatta
     1021 tgaaggaatt atgtttagag gatactaccc aaactcatat aaagaaattc ttagtggtgg
     1081 aagatatgat tctttaacta aagagtatgg aaaagaaatt ccagccattg gatttacact
     1141 aagtgttgat gagttaatga agtatgtata taagtaaagt ttagaaggag ggattgcttt
     1201 ggattatttg actatagcac tagcaaaagg acgaattgaa ggggaatcat ttaaaaaatt
     1261 taaaaaaatg ggtctaggag atagtataga cacagatacg agaaaactaa tttttaaaga
     1321 tgaagaaaat aaaattatat atatacatgt aaagccatca gatgtagtta catatgttga
     1381 aaaaggagtc gctgaccttg gtatagcagg taaagatact attttagaga atgagacaga
     1441 tgtatatgaa atatatgatt taggttttgg aaaatgtaaa tttgctgtag caggattaaa
     1501 gggagatagc atatatagag aagatgaata tctaaaagtg gcaacaaagt atcccaatat
     1561 agcaaaaaag tattttaaag agaaaggtca aaaaattgaa ataataaaat taaatggttc
     1621 tgtagaacta gctccaatag tagggttgtc agatgtaatt gtagatatag ttgagactgg
     1681 caatacactt aaagcaaatg gtcttgaaat attagaagac atatgtaata taagtgctag
     1741 aattatatca aatagagcta gctatagatt taaatatgaa caaattcaga atataattcg
     1801 tttatttgaa gaacttgata attaaaatat aattgaactt tataaggagg gatttaagtt
     1861 gagagaaaaa gaaagtataa gagagttaag gggatatgaa ccaaatcacg taaactgtaa
     1921 agtaaaactt gatgcaaatg aaggaagtaa gaggttgttt aaatacctta ttaaagaaat
     1981 ttcagatagt gatatagatt taaatttata tcctgaagat tcttatagtg atttgaaaga
     2041 atctataata gattacataa atatttctgg agtaaataag aaaaatcttc tagtaggaaa
     2101 cggttcaagt gaaataattg atttaataat acatactttt gtagataaag atgaagtaat
     2161 attgagtttc tctccaagtt ttagtatgta ttctatatat agtcagataa atgggtctaa
     2221 atttataggt gttgaaagtg atgaaaattt agttataaat atagataatg tgattgaaaa
     2281 agtaaaggag aataatccta aaatagtaat tgtatgtaat cctaataatc caactggaac
     2341 aatattaaag agggaagaga taataaagct tttagattca actaatagtc tagtggtttt
     2401 agatgaagca tatatggatt ttggagaaga aagcatgtta agtgatgttt ttaagtatga
     2461 taatctaata gttcttagaa ctttatcaaa ggcatttgga ttggctggaa taagaacagg
     2521 atatatgtta tctaatagta gtttaataaa ttctgttgag aaagtaagac ctccatataa
     2581 tttaaattca ttatcagact ttattgctac aagagcactt agaaataaag atgtggtaaa
     2641 ggcttatata aaggaagtaa aagaggaaag agaagtttta tatgaagaaa tgattggtat
     2701 gggaattaaa gcatacaaat cacaagcaaa ttttatatta ttttattctg aaatcgaaaa
     2761 tttatcacaa aaacttattg atagaggagt tttaataaga aaatttggtg gaaagttaga
     2821 aaattattac agagttacaa ttggtgataa agaagaaaat tctatgtttg ttggagctat
     2881 aagagatatt ttgaaaaagg agaaataggt atgagaattt ggaaggtaga aagaaatact
     2941 tttgaaacac agattttagt agaattaaat attgatggtt ctggtaaagc tgaaatagat
     3001 actggaatag gatttttaga ccatatgctt accttaatgt catttcatgg caagttcgat
     3061 ttaaaagtga tttgtaaggg tgatacatat gtagatgacc accatagtgt agaagatata
     3121 ggtatagcta taggtgaagc atttaaaaat gcactaggtg acaaaaaagg aatacgaaga
     3181 tattctaata tctatatacc tatggatgaa tctctatcaa tggtagcaat agatataagt
     3241 aatagacctt atcttgtatt taatgctaaa tttgataccc aaatgattgg tagtatgagt
     3301 actcaatgct ttaaagaatt ttttagagct tttgtaaatg aatcgagagt aacattacat
     3361 atcaatctct tatatgggga aaatgaccat cataagatag aaagtatttt taaagcattt
     3421 gcaagagctt taaaagaggg tagtgaaata gtttctaatg agatagcatc ttcaaagggg
     3481 gttttgtaga atgaatataa tagtagacta tggtcttgga aatatagatt ctgtgtctag
     3541 gggttttaga aaagctggaa tagaaactaa aatttctagt gatatagatg aaataaaaca
     3601 ggcagattca cttatacttc caggtgttgg agcttttaga gattctataa gtgccttaga
     3661 taaactagga ttgataccaa taataaagga gcatgtttct aagggtaaat ttatgatagg
     3721 tatatgtcta ggaatgcaat tactttatga aaaaagttat gagtatggag aatatgaagg
     3781 attaggactt ataaaaggaa gtattgataa actggatata actttaaaag tgccacatat
     3841 gggatggaat aatctaaaat ttaataaggc aaatgatgat atacttaaat atataaacga
     3901 agatgattat gtatacttcg ttcactcata ttatgcaaat tcatcaaatg aagaactagt
     3961 tgcattttca gaatatgaaa agaaaatccc tgctattgtt agaaaaggca atgtatatgg
     4021 tatacaattt catcctgaaa aaagtggaga agtagggtta aatatattaa gagcatatgg
     4081 ggagatgata aaatgataat atttccagca atagatataa aagataataa gtgtgtaaga
     4141 ctcacacaag gagaatttga caaagtaaat gtatattatg ataatccatt agaagtagca
     4201 tataaatgga aaaatgaagg agcagaatat atacatatag tagatttaaa tggtgctaga
     4261 agtgaatttg gtataaatac aaaaataatt gaggatatag caaacaatat agatatacct
     4321 attcaagttg gtggtggagt tagagataag ggaaaagtca aaagtttaat aaatgctggt
     4381 gttactagag taatattagg tagtatagcg attgaaaatt tgaatttggt tgaagaattg
     4441 gtcaatgaat ataaagagaa aattgtagtt tctatagacg ctaaagatgg aaaagtagca
     4501 gttaggggat gggaagttgt aagcgatgta gactcattaa cattatgtaa acaacttgaa
     4561 aagataggtg tacaaactat tgtttataca gatatatcaa aagatgggat gttgcaaggg
     4621 ccaaattttg acatatatga aagaatagca aaggaaacgt cattaaatgt tattgcttct
     4681 ggaggcgtaa cttctataga agacgtaaaa agactaaaag ctatgaactt atatggtgca
     4741 ataataggta aagcgcttta tgataaaaag atagacttta aggaggcaca acaattatgc
     4801 ttactaggag aataatacct tgtctagatg tgagaaatgg aagagttgta aagggaaaaa
     4861 aatttaaaga tatagtagat gttgatagtc cagaagtact tggaaaattt tatagtgatt
     4921 gtggtgcaga tgaacttgta ttctatgata taactgcatc aaatgaagag agaaagacat
     4981 ctttagaatt tgtgacaaaa gttgcagaaa atataaatat accattttgt gttggtggag
     5041 gagtaaataa attagaagat ttcactgata ttttaagaaa aggtgctgat aaggtttcta
     5101 taaattcatc agcagtcaaa aatcctgaat taataaggga agcttcatta aaatttggag
     5161 cacaatgtgt tgttttatcg atagatgcaa agaaaaatga agagggttca tggagcgtat
     5221 acgtaaaagg tggaagagaa aaaactaatc tggatgcaat tgaatgggct gttaaaggtg
     5281 tagaacttgg agcaggagaa attgttgtaa atagtatgga cgaagatgga atgaaaaatg
     5341 gatatgatat agaattatta tcaaagataa cttctttagt aaatgtacct gtcatagcat
     5401 ctggaggcgc tggaaaaaag gaagattttt atgaggcagt taataaatct aatgtagatg
     5461 gaattttagc agcatctgtt tttcattttg gcgagataaa aataaacgat ttaaagaaat
     5521 atttaaaaaa catgggtgta gaagtaagat tataagttaa atactaaaag gagattagaa
     5581 atatggataa taagtgtaac aatgtataca gtgatgaagt agaggaattt ataagaagta
     5641 taaagtttga tgataaaggg ttagtccctg ttgttgttca agaggtagtt agtaaagatg
     5701 tactgatgtt agcatatatg aataaagatg ctataaagaa aaccttaaaa gataaagtag
     5761 cttgctattt tagtagaagt agacaaaaac tttgggttaa aggtgagaca tctggtaata
     5821 ctcaaaaagt agttaagatg tcttatgatt gtgatgtaga tactatacta ctttttgttg
     5881 aacaaactgg tgtagcttgt catacaggca attattcttg tttttacaga gacttgtttg
     5941 atgatagttc aaaaatggag cctgaagtac aaacaaatat attgaaagaa ttatatgatt
     6001 taatcaatga aagaaaaaat aatccagttg aaggttctta tactaattat ctttttgaaa
     6061 aaggaataga taagattctt aaaaaagtag gtgaagagag tagtgaggta attatagcat
     6121 caaaaaatac cgataaaagt gaacttattt acgaaattag cgatttagtg tatcatacat
     6181 tggttttaat gatagaaaaa ggtgttgaaa ttgatgaaat aaaaaaagaa ttacttaaaa
     6241 gaagaaaata gaataggata gattaatggt aataagaaaa tataccgctt ttaataaaaa
     6301 gtggtatatt ttttatttta tattttcgat ttatagaata atatttaaat tact
//
LOCUS       DAMJWP010000201         6295 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.208, whole genome shotgun sequence.
ACCESSION   DAMJWP010000201 DAMJWP010000000
VERSION     DAMJWP010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6295
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.208"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            178..1068
                     /locus_tag="PC700_003047"
     CDS             178..1068
                     /locus_tag="PC700_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDF2582322.1"
                     /translation="MSSTIKSNRIKGIMFIIASALGFAMMSAFVKLAGDLPSFQKVFF
                     RNLVSAIIAFWLIIKHKGSLTGKKENLKVLLYRSIFGTLGVIFNYYAIDKLMLSDANM
                     LNKMSPFLVVIFCAIALKEKINVKQIGAIIIAFIGALFIIKPTFSVEVIPYLGGIAGA
                     VFAAMAYTCLRVLGDREDYYTIVFFFSVFSLVTVGPIAFAVYEPMTLMQLIYLLLGGV
                     FASLGQFGITLAYKYAPAKEISIFDYSNIIFSAILSIFLFNVYPDMLSVVGYLIVFSA
                     AFYMFLYNKKLDKLDKEKKK"
     gene            1286..2572
                     /locus_tag="PC700_003048"
     CDS             1286..2572
                     /locus_tag="PC700_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDF2582323.1"
                     /translation="MEKVKVAVIGAGNRGTYAYAPYIYENSDICEIVAVAEPKKGRRE
                     LFTQKYNLDSKNVFETLEDFFKHDKMADAVIIATNDDRHYDVAKLALEKGYHVLLEKP
                     MSNSLDGLVHIDDLCDKYKDKIFMICHVLRYSPFYNKLKEIVESKKLGELVSIQYNEN
                     IGYWHFAHSFTRGNWRNSNETSPLILAKSCHDMDILLYLVGSRCKKISAFGSLKHLNN
                     QNASGEMAQNCLQCLVEKKCPYSAKRIYLEKDRSINRAVHINPTEENLLNILKTSPYG
                     RCVYRCDNNVVDNMVNILQFENGVTATFNLCAFTKENGRTIKLMFSHGEVGGDLNKNE
                     IRIKKFGKNEEIVMNPSNQQNMVEEYDRNLIAEFIKLVSNKELEKGRVAAKEAIQSHV
                     MAFAAEYSRVSDEVVYIEEFFDSAKQMTKEIEETIF"
     gene            complement(2661..3104)
                     /locus_tag="PC700_003049"
     CDS             complement(2661..3104)
                     /locus_tag="PC700_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory YrvL family protein"
                     /protein_id="HDF2582324.1"
                     /translation="MIKISNKRRIPLGEFISVFLFFAVFFGITFCIFTVIGIGFLSFL
                     GFEYKSLGAVLIFFLIYFCITTPIDFLCTTILDIFRYVNKLPYSIYKLCEFIIDFILT
                     FLAMNIIDTFMDSVTIPLSTEILFALLSYLLSECMDFFDRDKKKP"
     gene            complement(3128..3922)
                     /locus_tag="PC700_003050"
     CDS             complement(3128..3922)
                     /locus_tag="PC700_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HDF2582325.1"
                     /translation="MKLYLSADIEGTCGIVNWDETKLECEFSQHYRTQMSKEITAACD
                     GATQSGVHEILVKDAHDSGRNINPNILPENVKILRGWTKDPLVMMAGIDESFDACIFT
                     GYHSGATCNGNPLSHTMDIDYDYFKINDEIASEFTINAYIAAYYNVPVAFLSGDEMLC
                     ESAKKLNPNIVTVPVSKGIGNGSISIHPNLALKKIEKGVKKALSGDLSRHLIKLPDKF
                     KIEIKFREHYKAFKASFYPNMKKIDSQTVLFETEDYYEFLRMLLFI"
     gene            complement(4488..4964)
                     /locus_tag="PC700_003051"
     CDS             complement(4488..4964)
                     /locus_tag="PC700_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDF2582326.1"
                     /translation="MKEILINPIGKICIQGEEVFIKLDKKYVPALNELDDFSHLNVFW
                     WADGFDNPELRSILETPKPYKSGPNVIGIFATRSPIRPNPIALTAVQIINIDHENGII
                     KIPYIDANDNSPVIDLKPYTPSVDRVENPSVPKWCSHWPKSVEKSGEFNWEDEIEF"
     gene            complement(5065..6234)
                     /locus_tag="PC700_003052"
     CDS             complement(5065..6234)
                     /locus_tag="PC700_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2582327.1"
                     /translation="MLNIRTLCHEINDWIINIRRDLHKTPELGLEEFQTKKKIIKYLN
                     EIGINYIEYKNHTGITAYINVSPNFETVAIRADIDALPITEELNYSYKSINIGKMHAC
                     GHDAHTAILLGTCNILFKLKNYLNVNVKFFFQPAEETIGGAQLMIEDGCLENPNVKYI
                     FGLHVNPNINKNLIELKYNTLNASTDTLQLTVHGSKCHGAYPHQGIDAIVISAHIITA
                     LQTIVSRNTNPTDSVVISLGKIEGGIKENIVCDKVVIRGTLRTLTPETREFSKKRIRE
                     ICDFTCKTFGGSISVEIEEGYPALINSNHLVDYVKQNAVELFGEENIILKDSPTLGAE
                     DFSYFLRHCEGAFYHLGCANREKNITSPLHTSTFDIDEDCLITGVMLHVKNVLSF"
BASE COUNT         2337 a          796 c          948 g         2214 t
ORIGIN      
        1 ataacttttt agctataatt attattgtta ttaattgaaa agacttatta gttacttgac
       61 atagtttaca cttttaatta tagtataaat tggaatattt agataattat tatttaatgt
      121 ataagctaag tttttaatta atatggcttt atacatttat atagaaaaga ggtacgtatg
      181 agtagcacaa tcaaatcaaa tcgaataaaa ggtataatgt ttataatagc atcagcgctt
      241 ggctttgcaa tgatgtctgc attcgtaaag ctagctggag atttaccaag ttttcaaaaa
      301 gtgttcttta gaaatctggt atcagctata atagcttttt ggcttatcat aaaacataaa
      361 ggaagtctaa ctggtaaaaa agaaaattta aaagtacttt tatataggtc aatatttgga
      421 actctaggag ttatatttaa ctactatgca attgacaagt tgatgctttc agatgctaat
      481 atgctaaata aaatgagtcc cttccttgta gtaatatttt gtgcaattgc tctaaaagaa
      541 aaaataaatg taaagcagat aggagctatt ataattgcat ttataggagc tttattcata
      601 ataaaaccaa catttagtgt agaagtaatt ccatatcttg ggggaattgc aggagctgta
      661 tttgcagcaa tggcatatac ttgtttaaga gttcttggag atagagaaga ttactataca
      721 attgtatttt tcttttcagt tttttcatta gttactgtag ggccaattgc ttttgcagta
      781 tatgaaccaa tgacccttat gcaacttata tatctattac taggtggagt atttgcaagt
      841 ctaggtcaat ttggaataac tttagcatat aaatatgcac ctgctaagga aatctctata
      901 tttgactata gtaatataat cttttcagca attttaagta tattcttatt caatgtctat
      961 ccagatatgt taagtgttgt tggatattta atagtatttt cggcagcatt ttatatgttt
     1021 ttatacaata agaagttaga taaattagac aaagaaaaga agaagtaaac tagataaaag
     1081 tgtttttata atttgtattg aatatttaag tctttgtatt aaaattaaag aaaatttaat
     1141 taaaaatgtt tggtatatta acttataaca gcagttaaaa ttgaaaaatt ataactgctg
     1201 tttcttttat taaaaatatc caaatgtctg taaaaataga ctataataga gtagtataaa
     1261 taatataaag gggataaatt aaagtatgga aaaagtgaaa gttgctgtga ttggagcagg
     1321 taatagagga acgtatgcat atgctccata tatttatgaa aattctgata tatgtgaaat
     1381 agttgctgta gcagaaccta aaaaaggaag acgagaacta tttacacaaa aatataacct
     1441 agatagtaaa aatgtatttg aaactttaga agactttttt aaacatgaca agatggcaga
     1501 tgcagtaata atagctacaa atgatgatag acattatgat gtagcaaaac tagctcttga
     1561 aaaaggttat catgtactat tagaaaagcc aatgtctaat agtttagatg gtttggtaca
     1621 tatagatgac ttgtgtgata agtataaaga taagattttt atgatttgcc atgtactgag
     1681 atattctcca ttttataata aattaaaaga aattgtagaa agcaaaaaat taggagagct
     1741 agttagtata caatataatg aaaatatagg atattggcat tttgcacata gctttactag
     1801 agggaattgg agaaatagca atgaaaccag ccctttaatt ttagcaaaga gttgtcatga
     1861 catggatatt ttgttatatt tagtaggaag tagatgcaaa aaaatatctg catttggaag
     1921 cttaaagcat cttaataacc aaaatgctag tggagagatg gcacaaaatt gtcttcagtg
     1981 tttagttgaa aaaaaatgtc catattcagc aaagagaatt tatttagaaa aagatagaag
     2041 tataaataga gctgttcaca taaatccaac tgaagagaat ctattaaata tacttaagac
     2101 aagtccatat ggaagatgtg tttacagatg tgataataat gttgtagaca atatggtaaa
     2161 tatattacag tttgaaaatg gagtgacggc tacttttaat ttatgtgcat ttactaaaga
     2221 aaatggaaga actataaagt taatgtttag tcatggtgaa gttggtgggg atttaaataa
     2281 aaatgagata agaattaaaa aatttggaaa aaacgaagaa atagttatga atccatcaaa
     2341 tcaacagaat atggtagagg aatatgatag aaatcttata gctgaattta ttaaattagt
     2401 ttcaaataaa gaactagaaa agggtagagt agcagcaaaa gaagctatac aaagtcatgt
     2461 aatggcattt gcagcagaat attctagggt cagtgatgaa gtagtgtata tagaagaatt
     2521 ttttgatagt gctaagcaaa tgactaagga gatagaagaa actatatttt aattaataaa
     2581 aatttattaa ataaaataaa gatagattaa ataatagaaa tacgaaaata tgtgttctat
     2641 taaggaatct atctttttta ttaaggtttt ttcttatccc tatcaaaaaa atccatacat
     2701 tctgataaaa gataagaaag taaagcgaat aagatttctg tacttaaagg aatcgttact
     2761 gaatccataa aggtatcaat tatgttcata gctagaaaag ttagtataaa atcaattata
     2821 aattcgcaaa gtttatatat tgagtatggt aatttgttta catatctgaa tatatctaaa
     2881 atagtagtgc atagaaaatc aattggagtg gttatacaaa aatatattaa aaagaaaatt
     2941 aatacagctc caagagattt atattcaaat cctaagaatg ataagaagcc aattcctata
     3001 accgtgaaaa tacaaaaagt tataccaaaa aaaacagcaa aaaataaaaa tacagaaata
     3061 aattcgccta aaggaatcct tcttttatta cttatcttaa tcaactcctt aatttatgat
     3121 atgaaattta tataaataat aacattctta aaaattcata atagtcctct gtttcaaata
     3181 gtactgtttg agaatctatt tttttcatat ttggataaaa agaggcctta aaagctttat
     3241 aatgttctct aaattttatt tctatcttaa atttatctgg caattttatt aagtgcctag
     3301 ataaatctcc agataaagct tttttgacac ctttttcaat tttttttagt gcaaggtttg
     3361 gatggattga aattgaacca tttccaattc cttttgacac aggaacagtg actatatttg
     3421 gatttagttt ttttgcactc tcacaaagca tttcatctcc acttaaaaat gctacaggaa
     3481 cattataata tgcagcaata taagcattta tagtaaattc agatgctatt tcatcattta
     3541 ttttaaaata gtcataatca atatccatag tatgagaaag tggattacca ttacaagtag
     3601 ctccagagtg atagccggtg aatatacatg catcaaagct ttcatcaatt ccagccatca
     3661 taactagtgg gtctttggtc catcctctaa gtattttgac gttttcaggt aaaatatttg
     3721 gattaatatt tctaccactg tcatgtgcat ctttaacaag tatttcatgt acaccagatt
     3781 gagtagctcc atcacatgct gctgttatct ctttactcat ttgtgttcta tagtgttgtg
     3841 aaaattcaca ttcaagtttt gtttcatccc aatttacaat accacaagta ccttctatat
     3901 cagcacttaa atacaatttc ataatatata cccccttatg tttattattg atgataataa
     3961 cagataacag aaaatacata atcaataaag ggcataaaaa tattatgtct tactattaat
     4021 tattattaaa tctgtaactg aaaaataact aaattggtat taaaaaatat aacactatgt
     4081 gtcatttatt tatatagttt tttatatctg atgtttatat aaaattgtaa catataagtg
     4141 gttacattta ctagtttaac atagttaaaa tagttaggta ttatttttaa gttaaattga
     4201 ttattataaa atcgtagaat aaaagaaaaa tttaatttat taaaatgcat gaaatgtttt
     4261 ttcctcaaca taatgctaaa ttaaactcaa aaagaaataa tagaaaaatc ttatgtagct
     4321 tagaaagaat gtgaatgatt taagggaaaa atagaaattt attatataat attaggctgt
     4381 attatgtaat acagcctaat attatatgta tatgtgacat aaaatcttgg taagtgtaaa
     4441 taaaataaat gtggaaaaca aattaatgaa atcaagaaaa aagtaattta aaattcaatt
     4501 tcatcttccc aattaaattc tcctgatttt tctacactct taggccagtg actacaccat
     4561 ttaggaacgg acggattttc aaccctatca acgcttggag tatatggctt taagtctata
     4621 acaggactat tatcattagc atcaatatat ggtattttta ttataccatt ttcatggtcg
     4681 atattgataa tttgtacagc agtcaatgct attggattgg gtcttatagg tgaccttgtt
     4741 gcaaatattc ctataacgtt tggtccactt ttataaggtt ttggggtttc taaaatactt
     4801 cttagttcag gattatcaaa cccatcagcc caccaaaata catttaaatg actgaaatcg
     4861 tcgagttcat ttaatgcagg tacatacttt ttgtctaatt taataaagac ctcttcgcct
     4921 tgaatacata ttttacctat aggatttatt aatatttctt tcataatatt acctccaact
     4981 atttaattta tcatttgtaa ttttagggta taacatgaca tcatgtgagg gtcaatataa
     5041 aataataaat aaaaatatta atagttagaa tgataataca ttttttacat ggagcatgac
     5101 accagtaata aggcaatctt cgtcaatatc aaaagtagag gtatgcaaag gggaagtaat
     5161 gtttttttca cgatttgcac aaccaaggtg gtaaaaagct ccttcacagt gtcttagaaa
     5221 atacgaaaag tcttcagccc caagagtggg cgagtcttta aggataatat tttcttctcc
     5281 aaacaattct acagcatttt gcttgacgta atctactaga tggtttgaat tgataagagc
     5341 tggataacct tcctcaattt caacagaaat acttcctcca aatgttttac aagtaaaatc
     5401 gcaaatttct ctaattcgtt tttttgaaaa ctctctagtt tcaggagtca aggttctcaa
     5461 agtacccctt ataaccactt tgtcacaaac tatattttcc ttgattccac cttcaatctt
     5521 tcctagagaa ataaccacag aatcagttgg atttgtgttt cgactaacga ttgtctgtag
     5581 agctgtaata atatgagcag atataacaat agcatcaata ccttgatgtg gataagctcc
     5641 atgacatttt gaaccatgta cagttaattg aagtgtatcg gtggaagcat ttagagtgtt
     5701 gtattttaat tcaattagat ttttgttgat attgggattt acatggagtc caaatatata
     5761 ttttacattt gggttttcaa gacatccatc ttctatcata agttgagccc caccaatagt
     5821 ttcctcggca ggttgaaaaa agaattttac atttacattt aagtaattct ttaatttaaa
     5881 tagtatatta caagtaccca gtaaaatagc agtgtgtgca tcatgaccac atgcatgcat
     5941 tttaccaata ttaatagatt tataagaata attaagttct tctgttatag gtaaggcatc
     6001 tatatcagct cttatagcaa ctgtttcaaa gtttggactc acgttaatgt atgcagttat
     6061 ccctgtatga ttcttatatt caatataatt tatacctatt tcatttaagt atttaataat
     6121 ttttttctta gtttgaaatt cttctaatcc aagttctgga gtcttatgta aatctcttct
     6181 tatatttata atccaatcat ttatctcatg acaaagagtt cttatattaa gcataaaaca
     6241 catcctaact atattaaaaa tttatttttc ttcattaatt atattatttt atgta
//
LOCUS       DAMJWP010000202         6257 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.209, whole genome shotgun sequence.
ACCESSION   DAMJWP010000202 DAMJWP010000000
VERSION     DAMJWP010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6257
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.209"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..1660
                     /locus_tag="PC700_003053"
     CDS             <1..1660
                     /locus_tag="PC700_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582328.1"
                     /translation="DIDVNIFNLNSEKDRINRHIDDTESKINKFKIYEPIKLENAKLE
                     ELEIKYKKYLEDPTKKEIETLNYEISVCLKRIESITIELEEFRQLISSDNFENIPILF
                     TIEELNKRIVLNKSKVDELRKLKYSIKSDIDKNQGSLNTKREEILKKFNGKEPLSTSL
                     IVNFNFEERKIDIKKDIKDIGEIIEGNRTTCQNLEQELRDLKKYKVERKAITYQPEDI
                     FIESKKLQSKLQNVEYNLIKSINSYKNTILEIKEFNYKHMEKYKNFIETLNVNKDNYS
                     RQKEQVEIVLLVIREEISKLEKHSEQLRKEKTIISRQIKDYVMDCIDEFKIINKLGKH
                     KGQALFNISLPKSDKIENSLSLVDMLMDEIVEEANLGDVEQKINTFYILNKTLNIGRI
                     PVSVIKYEINRNETIKWNSINNETTGGQRFCISFIVTILLMEYKRYDRNAIVDESKYK
                     GKVLLMDNPFGETSQQDFLKEIFELAKKFNVQIISYTHVTNASIRAMFNKIYLMTVEK
                     TTSNKEFVDINEIKQNKTDELVYMSKFYIGNKEELIQEDIFSLI"
     gene            1762..1971
                     /locus_tag="PC700_003054"
     CDS             1762..1971
                     /locus_tag="PC700_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582329.1"
                     /translation="MKNFVIVSTKAALLRVIIVRLLLKIGLKTYKYLSYFMKKEKLKR
                     IIRPEGCELRVNRSIQVERAIAEQI"
     gene            2367..2507
                     /locus_tag="PC700_003055"
     CDS             2367..2507
                     /locus_tag="PC700_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2582330.1"
                     /translation="MKIKIKKYREKLLMIQNELAKSVGVRRETIVHLENGLMYHKGIQ
                     DK"
     gene            2645..3607
                     /locus_tag="PC700_003056"
     CDS             2645..3607
                     /locus_tag="PC700_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017561613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HDF2582331.1"
                     /translation="MAKIDNMMSILWMLNSDKKVTAKQISEKLEINIRTVYRHIDALS
                     ASGVPIISDTGHNGGYTLLNNFIKAPLFFDTEEQTALLHAAIFAKEEGSFLDDTLDKA
                     TSKLKMYSNQKQEETLTKHLIGFEVIKPTGKISIESVLKKLKNSIANELSVEINYRTA
                     HEEKSKCRIINPYGIVRWNNNWYIVAFCQMRNEIRSFRVDRISSVIETSNTFNRPKEF
                     SASEFFIRGIMDETKDEDNLISLVIEGKMNALDNLCQHWLLGYYLKERTSNKAIFKLQ
                     EEIIFTYVPNILIPYRKSIQIIEPLSLRDKMVEDLLELINHYQI"
     gene            3685..4314
                     /locus_tag="PC700_003057"
     CDS             3685..4314
                     /locus_tag="PC700_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HDF2582332.1"
                     /translation="MKKRKVYMYVFDTMSDWEVGYLTAELNTGRYFKKGLKPLKVITV
                     GVDKNPIITMGGLKVLPEITIDELNIESNDLLILPGGNTWTSIGHEKILEKSKEALEQ
                     RSIVAAICGATLGLAKTGLLDFRNHTSNDLEFLKMVILSYSGEKYYKMESAVNDENLI
                     TASGIAPLEFTVQVLKVLDVFKEEALSSWLNLYKTHDSKYFFELMNCNK"
     gene            5145..5561
                     /locus_tag="PC700_003058"
     CDS             5145..5561
                     /locus_tag="PC700_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582333.1"
                     /translation="MSKSETGVKSKLILELLVITYLFIAVLATKYIFKGPSTASLPGL
                     SWLIVFGCYSIYNKKYNDLVDESSKFILSKVNQIAITIMMYSIVIIAIFFATPYISNI
                     NISNLDTGIILIAVLFIQSLSKLILYIYFDKRGICN"
     gene            5562..5774
                     /locus_tag="PC700_003059"
     CDS             5562..5774
                     /locus_tag="PC700_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2582334.1"
                     /translation="MAISMKTKIKEYREKLLMTQNELAKSVGVRRETIVHLENGKYNP
                     SLKLAMDIAKVFGTTVENLFEFIEED"
BASE COUNT         2619 a          618 c          970 g         2050 t
ORIGIN      
        1 agatattgat gttaatatat ttaatttaaa ttcagaaaaa gataggataa ataggcatat
       61 agatgataca gaaagcaaga taaataaatt taagatttat gaacctatta aacttgaaaa
      121 tgctaaacta gaagaattag aaataaaata caagaaatac ttagaagacc ctacaaaaaa
      181 agagattgaa acattaaatt atgaaatctc agtttgctta aaaagaatag agagtattac
      241 tatagagtta gaagaattta gacaacttat tagtagtgat aattttgaaa atatacctat
      301 attattcaca atagaagaac ttaataaaag aatagtttta aataaaagta aggttgatga
      361 actacgaaaa cttaaatata gtattaaaag cgacatagat aaaaatcaag gttcattaaa
      421 cactaagagg gaggagatac ttaaaaaatt taatggaaaa gaacctttat ctactagctt
      481 aatagtgaac tttaattttg aagaaagaaa aatagatata aaaaaagata taaaggatat
      541 tggtgaaatt atagaaggta atagaactac ttgtcaaaat ctagaacagg aattaagaga
      601 cttaaagaaa tataaagtag aaagaaaagc tataacctat caacctgaag atatttttat
      661 tgaaagtaaa aaactacaat caaaactgca aaatgttgaa tataatttaa ttaaatctat
      721 aaatagctat aaaaatacaa tattagaaat aaaagaattt aattataagc acatggaaaa
      781 atacaaaaac tttatagaaa ctttaaatgt aaataaagat aattactctc gacaaaaaga
      841 acaagtagaa atagtattac tagttataag agaagaaata agcaaattag aaaaacatag
      901 cgaacaacta agaaaagaaa agactataat atcaaggcaa ataaaagatt atgttatgga
      961 ctgtattgat gaatttaaaa tcataaataa acttggtaaa cataaaggac aagccttatt
     1021 caacatatca ctacctaaat ctgataaaat agaaaatagt ttgagcttag tagatatgct
     1081 aatggatgaa attgtagaag aagcaaacct aggtgatgtt gaacaaaaaa taaatacctt
     1141 ttatatttta aataagactc ttaatatagg gagaattcct gtatcagtta taaaatatga
     1201 aataaataga aatgaaacta taaagtggaa ctctattaat aacgaaacaa caggaggaca
     1261 gagattttgt atctcattta ttgtgacaat attgttgatg gaatataaaa gatatgatag
     1321 aaatgcaata gttgatgaaa gtaaatataa gggtaaggta ctacttatgg ataatccttt
     1381 tggagaaacg tcgcagcaag atttccttaa agaaatattt gaacttgcta aaaaattcaa
     1441 cgttcaaatt atatcctata ctcatgtaac aaatgcttct ataagagcaa tgtttaataa
     1501 gatttattta atgacagttg aaaaaactac ttctaataaa gaatttgtgg atattaatga
     1561 gattaagcaa aataaaactg atgaattggt gtatatgagt aaattttata taggtaataa
     1621 agaagaatta attcaagagg atatatttag tttaatataa ataacaagta aacaaataaa
     1681 gtgattacta tcagtgaggc aggtagaaac tgaattactt tgtgactcat tacgactctt
     1741 ggggtaaaat tgtgtataaa tgtgaagaat ttcgtgattg tcagtacaaa agcagcttta
     1801 ttaagggtaa taatagtaag actcctcttg aaaataggac taaaaactta caagtatcta
     1861 agttatttca tgaaaaaaga aaaattaaag agaattataa gaccagaggg atgtgaactt
     1921 agagtgaata gaagtataca agtagaaaga gctatcgcag aacaaattta attttatcat
     1981 acgatagcct cttgttattt tagtgatgaa atccatcaag cataccataa ctacagaaaa
     2041 agaggctttt ctgatggtat attgggttct tttagagtgt gtgatttgct acatgttcat
     2101 taggataata atagtcagta aatgatatta ctgatagaat agatataata aactaatcta
     2161 gttccatatt gtaatgttta ttaaagagta tacttttatg attataatag ctattttctt
     2221 tgaagcttat tgtacaagag atttgaatat atcaaatttg gatataggta taattctctt
     2281 agcagtttta tttatacagt ctttatctaa attagtgttg tatatacttt gataaaaggg
     2341 ggatatgtaa ctaagttgta atattgatga aaataaaaat taaaaaatat agagaaaaac
     2401 tccttatgat acaaaatgaa cttgctaaat cagttggggt aagacgagag actattgtac
     2461 acttagaaaa tggattaatg taccataaag gtatccaaga taaatagtta aaaaactaga
     2521 taagaataag gagcctttac agaacaaatt tagtgtgctc gtgcgatagc ccttttttta
     2581 taaaaattaa tctaataacc tatataatta aatttataaa acatatatga aagggtaaaa
     2641 ctatatggct aaaattgata atatgatgtc catattatgg atgttaaact cagacaaaaa
     2701 agttaccgca aaacaaatat ctgaaaaact agaaataaat ataagaactg tctacagaca
     2761 cattgatgca ttatctgcta gtggagtacc tataatttca gatacaggtc ataatggtgg
     2821 atatacactt ttgaataatt tcatcaaagc acctcttttt ttcgatacag aagaacaaac
     2881 tgcgttactt catgcagcta tatttgctaa agaagaggga agtttcttag atgacacact
     2941 agacaaagca acctcaaagt taaaaatgta ttcaaatcaa aaacaagaag aaactttaac
     3001 aaaacattta ataggttttg aagtaataaa accaactgga aaaatatcca tagaatctgt
     3061 acttaaaaag ttaaaaaata gtatagcaaa tgaattatct gtagaaatca actatcgtac
     3121 agctcatgaa gaaaaatcaa aatgtaggat tataaatcca tatggaattg tacgttggaa
     3181 taataattgg tacatagtag cattttgtca aatgagaaat gagattcgta gttttagggt
     3241 agatagaatt agtagtgtca tagaaacatc aaatactttt aataggccaa aagaattttc
     3301 agcaagtgaa ttttttataa gagggattat ggatgaaaca aaagatgagg ataatcttat
     3361 atcattagtt atagaaggaa agatgaatgc attggataac ttatgtcagc attggcttct
     3421 aggatattat ttaaaagaaa ggacttcaaa taaagcaata tttaaacttc aagaagagat
     3481 aatatttaca tatgtaccga atatacttat accttataga aaatctatac aaataattga
     3541 accattaagc cttagggata aaatggttga agatttactc gaattaatta atcattatca
     3601 aatttaataa cttaactgac agagtatgtc agttaagtta ttttacaatt aggtatatta
     3661 attaattata gaaggagcaa aaaaatgaaa aagagaaaag tatatatgta tgtattcgat
     3721 actatgtcag attgggaagt tggatatcta actgctgaat taaatacagg aagatatttt
     3781 aaaaaaggat taaaaccact aaaagtaatt acagtaggag ttgataaaaa tcctattata
     3841 acaatgggtg gattaaaagt tttacctgag attacaattg acgagcttaa tattgaaagt
     3901 aatgacttat taattttgcc agggggaaat acttggacat ctataggtca tgagaaaata
     3961 ttagaaaaat ctaaagaggc tttggaacaa aggtctatag ttgcagctat ttgtggtgct
     4021 actttagggt tagctaaaac aggtttacta gattttagaa atcatacaag taatgattta
     4081 gaatttttaa aaatggtgat actaagttat tctggagaaa aatattataa aatggaatct
     4141 gcggttaatg atgaaaattt aattactgct tctggaattg caccattaga atttacagta
     4201 caagttttaa aagttttaga tgtttttaaa gaagaagcat taagttcttg gttgaacctt
     4261 tataagacgc atgactctaa atattttttc gaactaatga attgtaataa ataaatttaa
     4321 gatttaattt taaattcaaa ttttaaatta ctttttctat ggcaatagat aagtagttta
     4381 gaattttatc atatatataa tttgtattta taccaacaca aatagaaatg atagttccaa
     4441 taataaaatt tataattagt tccatatgga gtacatttct ttcctactaa tataactaag
     4501 aatatatgaa accaccatag attgtagttt tttctgttgt attatatcat gggatagtaa
     4561 agtgacgtat atttttgatg aaaacctagg attgtatatg aaatgaatta atcctgtgta
     4621 ttaatcttga gattcgtgtc taatgatttg taaaaataaa taatgaaata cttagaaata
     4681 cttgtaaatg ctagtcaata ttggctagta ttttttattt tagtaatgaa atccccaact
     4741 gtacttcatc cacaaaaaaa gagatatttt tgagggtgta ttggagtgaa cttaggctgt
     4801 ataattatac atttcaatat cttatattaa ttaggataat agttggtaaa tgatatgatt
     4861 gaaacaatag atataataaa ctaatctagt actgtactgc ttactaaaga atatattttt
     4921 atggtataag aattttttag agcgttgatt ctataggagt gaataaaaaa gtaattttat
     4981 aatattattt gtaaccatta gatgtctatt atgaaatctt ttatggacta tatttgtaaa
     5041 aatattatta agtatttaca atattttctt gcaatttatt tggaaaggtt atatatttat
     5101 aacatgaagt gttataaata tataaccttt aaggtggatt ttatatgtct aaatcagaaa
     5161 ctggtgtaaa atcaaaactc atattggaat tacttgtaat aacttatctt tttatagctg
     5221 tattagctac taagtatatt tttaaaggtc caagtactgc atctcttcca ggactgagct
     5281 ggttaatagt ctttggatgc tattcaattt ataataaaaa atataatgat ttagttgatg
     5341 aatcatctaa atttatacta tcaaaggtta atcagattgc aataacaata atgatgtatt
     5401 ctatagttat aatagctatt ttctttgcaa caccttatat aagtaatata aatatatcaa
     5461 acttggatac tggtataatt cttattgcag ttttatttat acaatcgtta tctaaattaa
     5521 ttttgtatat atactttgat aaaaggggga tatgtaacta agtggcaata tcgatgaaaa
     5581 caaaaattaa agaatataga gaaaaactcc ttatgacaca aaacgaactt gctaaatcag
     5641 ttggggtaag acgtgagact attgtacact tagaaaatgg aaaatataat ccttctttaa
     5701 agttagcgat ggatattgct aaagtattcg ggaccacagt agaaaatttg tttgaattta
     5761 ttgaagaaga ttagtagata ggatataaat atttataaga ttgttaataa aatatagtta
     5821 tttaattatt aattttgtta aaaatattta taaataatat aatgtatgag taaagttagg
     5881 ttgtaattat tttaagataa gaagactact gtaaatgata aaaattagtg aatatacagc
     5941 caattaataa ataatagata aaactttaaa gcttagatgt gaggatgaga tatggtctta
     6001 cagtagttca cacttttaat taagacttaa aatggaaacc atacacatac atacactatt
     6061 tactgaaagt ggactagata aaaaaataat aagtgattta agaaagtatc tcatatacta
     6121 tataagtatt attttaggtg acctatttat aggtcatttt ttatgtatat aaatgataat
     6181 aatactaata tttaaaatag gttttaaaga aataaaatat ccagattgaa aaatggatta
     6241 atgttaataa aaaaatt
//
LOCUS       DAMJWP010000203         6241 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.210, whole genome shotgun sequence.
ACCESSION   DAMJWP010000203 DAMJWP010000000
VERSION     DAMJWP010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6241
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.210"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            111..656
                     /locus_tag="PC700_003060"
     CDS             111..656
                     /locus_tag="PC700_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDF2582335.1"
                     /translation="MENVITGLSKGAEWFIGLFQKGGEQFIGLVSGTLPTLIVLMVAI
                     NSLIKIIGEERVNNWASKLGKNSFTRYILLPLVSVFFLGNPMCYTFGRFLKEEHKAGF
                     YDAAVSFVHPITGLFPHANAGELFVWLGISAGLTTLGKETTTLALWYFIVGLIVIFIR
                     GIVTEKMYAFLTRKNRATNKA"
     gene            669..1682
                     /locus_tag="PC700_003061"
     CDS             669..1682
                     /locus_tag="PC700_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDF2582336.1"
                     /translation="MEENKILKIEKGTSGWGGPLYIKKEGNRNKILSMTAGGIHEVTL
                     KIKELLGCEIVDGFKTGVSDEEVAVVIIDCGGTARCGVYPKKKIPTINVNPVGKTGPL
                     AKFITEEYYVSDVNPNCISVVDGEDMPQKSQENKSENKSSIRKPDNYDEVKSKAQGEY
                     AKKNIILSIGQGAGQVVSKFYDAGRDTIQMVMNNVIPFMAFVSMLMGIILASGLGDWI
                     ARVISPLAGNIGGLLIISVICTLPFLSPILGPGAVIAQVVGTLVGTQIGLGAIPAYLA
                     LPALFAINGQAGCDFVPVGLSLGEAEPETVEYGVPALFYSRLITGPIAVIIAYGVAVF
                     ALR"
     gene            1904..3877
                     /locus_tag="PC700_003062"
     CDS             1904..3877
                     /locus_tag="PC700_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDF2582337.1"
                     /translation="MSVTEKIPSIDRKIFKILIMCSKKEFVSINSIANELNVTTRSVR
                     TYIKQLNKDLGNDIAVIKYIKGQGYKLEIKDEQILNKIIDMNRKNVFSLNSKEDRVEF
                     ILNYLIELDGFITLDSLADEMCVGRTTLVNDFQYVEKVLASYNLNLIKKQNTGMKLNG
                     NELDIRLFILNQLYKNSRKDFSNSKYFKGIKKEEIINLEEKLLTLFKENNFYVTDEML
                     REVINYIVVLVYRVKEEKKVKDYDVKFDLLKSYDEYFIAREIKHIISGMFECTLNDEE
                     IIYLTIPLVSGNAPASECALNSSRISKNIDELMENIFNQIYVDMGIFINEDELRVGLG
                     YHLSFTLNRLLFNIKLKNVLLEEIKQNYILPFKLAQIAGKVIEKKYNLEVSEDEIGYI
                     AIHFSGYLERNSSRFYSIKKIAIICSTGLGTAKLLKIRVEKLIGNNPKIDTLSSFNLR
                     NINLDEYDIVFTTIDLDTSNINTIVLKINTIFDENKLREQLKTVLCLREGNIDTTNST
                     NLLINNLLDEDKFMILNEKTILASLEKMMDHLMDLGCIDDKFRKNIVNREEKSPTVFD
                     KGLLFPHSVNEKSDKFLMAVGILEEPIEYANRSIKIILMTMFPCENKIDSDLLVKIYE
                     EVLKIGQDVKLTNKISKCRTFLEFKKVLLKNLI"
     gene            3946..4305
                     /locus_tag="PC700_003063"
     CDS             3946..4305
                     /locus_tag="PC700_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582338.1"
                     /translation="MKLLVILFVAYILNTIFIMKQNKLYFKTLNRAKKKGDIVSTGKK
                     KSYFSKGSIAIISSDSDGFIKFGEILKGRTVMAKFKEIEEIEGLDIYSAEQKFKDEES
                     IVQAIKFIKEKINVSFN"
     gene            4340..4696
                     /locus_tag="PC700_003064"
     CDS             4340..4696
                     /locus_tag="PC700_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDF2582339.1"
                     /translation="MKYEVKISGIGSLVKELMDESNCLIIYDETINDDDLKDISVIHS
                     IATLKSDVEIGDTLTIGNRDYCIVSVGDIAQKTLREIGHCTIKFDGKCEVNLPGEIHV
                     EIGNPDITIGDLITIS"
     gene            4733..6025
                     /locus_tag="PC700_003065"
     CDS             4733..6025
                     /locus_tag="PC700_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDF2582340.1"
                     /translation="MLDLNRKLAKLEEEGKKIKVALIGAGHMGNGMVSQMANMKGIEA
                     SIVVDINLELAHKAFTDAGIDEEIIDNVTNEKDAEIKLQEGKVLTCNDFLVACKTKSI
                     DVVIDATGGIAIGAEIALNSILNKKHIVMLNAETDCVVGPILKKLADDAGVIFTGSAG
                     DEPGAVMELFDFADAMGFEVRVIGKGKNNKLDLDCNPDTVREEAERKGASPHMIASFK
                     EGTKTMVEMALMCNATGFVPDVRGGHGIEATVNEVPKKYALKSEGGVLDNYGVVDFVN
                     GIAPGVFVVVAHKLKAVNDELKYLSMGDGPNYILYRPYHLCSLETPLSAAMAVLDHKA
                     TIVPKAGLVAEVMTIAKKDLKKGEFMDGYGAYTCYGTIEKYDVAKAMNAVPIGLISKK
                     TKVVKDIKKGEVITYDMVEIEKDTTLYHLRQLQEKIFG"
BASE COUNT         2469 a          584 c         1184 g         2004 t
ORIGIN      
        1 aaaacattta caatattagc atatagtaca gaaaaaagat gtaatttgta aaatttatgt
       61 taagcagtaa aattaaaaat ttaaagatat tatagggagg ttataatttt atggaaaacg
      121 ttattacagg tctaagtaaa ggtgcagagt ggtttatcgg tttgtttcag aaagggggag
      181 aacaatttat aggtcttgtt tcaggtactc ttccaacgct aattgtatta atggtagcta
      241 ttaactcttt gattaagata attggtgagg aaagagttaa taattgggct tctaagctag
      301 gaaagaactc ttttacaaga tatattctac ttccattagt atcagtattt ttcttgggaa
      361 atcctatgtg ctatacattt ggtagatttt taaaagaaga gcacaaagca ggattttatg
      421 atgcagcagt ttcatttgta catccaatta caggtctttt tccacatgca aatgcaggag
      481 aactttttgt atggttggga atatcagcag gtttgactac tttaggtaaa gaaactacta
      541 cactggcatt gtggtatttt atagttggtc taattgtaat ttttataaga ggtatagtaa
      601 cagaaaaaat gtatgctttt ctaacaagaa aaaatagagc aacaaataag gcttaggagg
      661 tagataaaat ggaggagaat aaaatattaa aaatagaaaa gggaacttca ggctggggag
      721 gtcctttgta tataaaaaaa gaagggaata gaaataagat actatcaatg actgctggtg
      781 gtatccatga agtaacatta aaaataaaag aactactggg ttgtgaaatt gtagatggat
      841 ttaaaactgg agtttctgat gaagaagtag cagtagttat tattgattgt ggtggaactg
      901 ctagatgtgg agtttatcca aagaaaaaaa tacctacaat aaatgtaaat ccagttggaa
      961 aaacaggtcc tttagcaaaa tttataacag aagaatatta tgtatcagat gttaatccta
     1021 actgtataag tgttgtagat ggggaagata tgccacaaaa atctcaagaa aataaaagcg
     1081 aaaataagag ttctattaga aagcctgata attatgatga ggttaagtca aaagcacaag
     1141 gtgaatatgc taaaaagaac ataatacttt ctattggtca aggggcaggg caagtagtat
     1201 caaaatttta tgatgcaggt cgagacacta tccaaatggt aatgaataac gtaataccat
     1261 ttatggcatt tgtaagtatg cttatgggaa ttattttagc atctggactg ggagactgga
     1321 ttgcaagagt tatatctcca cttgcaggta atattggtgg attattgatt atatcagtaa
     1381 tttgcacact tccattctta tcaccaatac taggacctgg tgcagttata gctcaagtag
     1441 tagggactct tgtaggtaca caaattggtc ttggtgcaat accagcatat ttagcacttc
     1501 cagctttatt tgcaataaat gggcaagctg gatgtgattt tgtaccagta ggattaagtt
     1561 tgggagaagc agaaccagaa actgtagagt atggtgttcc agctttattt tactctcgtt
     1621 taataactgg tccaatagct gttattattg catatggagt tgcagtattt gcattgagat
     1681 aatatttaat gtcaatataa ggcttggaat taattaataa aattgtaaat ataaatttaa
     1741 gctagtcaag attttttgtt aatgaaagac tagcttttat actattaatt agctgtaatt
     1801 atcgagttag attaatacta aattttcctt tattaaagtg aaattttatg tattaatttg
     1861 gtttcaaatt tgtactagaa tgaaatcaag gaggagatac aaaatgagtg taactgagaa
     1921 aattccttct attgaccgca agatatttaa gatattgatt atgtgtagta aaaaagagtt
     1981 tgtgagtatt aattcaatag caaatgaatt gaatgtcaca actagaagtg taaggacgta
     2041 tataaaacaa ttgaataaag atttaggtaa tgatatagca gtaatcaaat atataaaggg
     2101 tcaaggctat aaactagaaa taaaagatga gcagatactc aataagatta ttgatatgaa
     2161 tagaaaaaat gtattttcat taaattcaaa agaagataga gtagagttta tactaaatta
     2221 cttgatagaa ctagatggat ttattacact tgatagttta gctgatgaga tgtgtgtagg
     2281 aagaactact ttagtaaatg atttccagta tgtagaaaaa gttttggctt catataattt
     2341 aaacttgatt aaaaaacaga atacaggtat gaaattaaat ggaaatgaac ttgatattag
     2401 actgtttata ttaaatcagt tgtataaaaa ctcaaggaaa gatttcagta attcaaagta
     2461 ttttaaagga atcaaaaaag aagaaatcat aaatttagaa gaaaaactat tgactttatt
     2521 taaagaaaat aatttttatg ttacagatga aatgttaaga gaagttatta actacatagt
     2581 agttttagtc tatagagtaa aagaagaaaa gaaagtaaaa gactatgatg taaagtttga
     2641 tttacttaaa tcatatgatg aatactttat tgctagggaa ataaaacata taatatcagg
     2701 aatgtttgaa tgtactttaa atgatgaaga aattatttat ttaactatac cattagttag
     2761 tggaaatgca ccagcatcag aatgtgcttt aaatagtagt agaattagta aaaacattga
     2821 tgagttgatg gaaaatatat ttaatcaaat atatgtcgat atgggtattt ttataaatga
     2881 ggatgaatta agagtaggtc taggatatca tcttagtttt actttaaaca ggctattatt
     2941 taatataaag ttaaaaaatg ttttgttaga agagattaag caaaattata tacttccatt
     3001 taaattagca caaatagcag gaaaagtaat agagaaaaag tataacttag aagtttcaga
     3061 agatgagatt ggatatatag caatccattt cagtggatat ttagaaagaa atagcagtag
     3121 attctattca ataaaaaaaa tagcaattat atgtagtaca ggtctaggaa cagcaaaact
     3181 attgaaaata agagtagaaa aactgatagg aaataatcct aaaattgata ctctttcaag
     3241 ttttaatttg agaaatatta acttagatga atacgatata gtttttacaa ctattgattt
     3301 agacacatcg aatataaata caatagtatt aaaaataaat acaatatttg atgaaaacaa
     3361 attaagagaa caattaaaaa cagtattgtg cctaagagaa gggaatattg acactactaa
     3421 ttctacaaat ttactaataa ataatttgtt agatgaagat aaatttatga tattaaatga
     3481 aaagactata cttgcttcat tagaaaaaat gatggaccat cttatggatt tgggatgtat
     3541 tgatgataag tttagaaaaa atatagtcaa tagagaagaa aaatctccca cagtatttga
     3601 taaaggttta ttatttcctc actctgtaaa tgaaaaatca gataagtttt taatggcagt
     3661 tggaatttta gaagaaccaa tagaatatgc aaatagaagt ataaaaataa ttcttatgac
     3721 aatgtttcca tgtgagaata agatagactc agacttatta gtaaaaattt atgaagaagt
     3781 tttaaagata ggccaagatg taaaattaac gaataagata agtaaatgta gaactttttt
     3841 agaatttaaa aaggtgttac ttaaaaacct tatataacta ttgtacagca tttatagttg
     3901 ttatacagat ttaaaaataa tgtgattttt ggaaggtgta taaatatgaa attattagta
     3961 attttatttg ttgcttatat tttaaatact atatttataa tgaagcagaa taaattgtat
     4021 tttaaaactt taaatagagc taaaaaaaag ggtgatatag tgtctacagg caaaaagaaa
     4081 agctattttt ctaaaggaag tatagccatt atatcttcag attcagatgg gtttataaag
     4141 tttggagaaa ttttaaaagg gcgtacagta atggctaaat ttaaagagat tgaagaaata
     4201 gaaggattag atatatatag tgcagagcaa aaatttaaag atgaagagag tattgtacaa
     4261 gctataaaat ttataaaaga aaaaataaat gtttctttta attaactcaa tataaatata
     4321 aattatcagg aggaatgtca tgaaatatga agttaagata agtgggatag gcagtttagt
     4381 taaagagtta atggatgaaa gcaattgtct tattatatat gatgaaacca ttaatgatga
     4441 cgacttaaag gatatatctg taattcattc gatagcaaca ttaaaaagtg atgttgaaat
     4501 aggagataca cttactatag ggaatagaga ttattgtata gtttcagttg gtgatattgc
     4561 tcagaagact cttagagaaa ttgggcattg tacaatcaaa tttgatggaa aatgtgaagt
     4621 aaatttacca ggagaaatac atgtggaaat aggaaatcca gatataacta ttggagattt
     4681 aataactatt tcttaatttg aaaataaaat tattataaca ggaggaattg gtatgttaga
     4741 tttaaacaga aaacttgcaa aattggaaga ggaaggtaaa aaaataaaag tagctcttat
     4801 aggagctggg catatgggaa atggaatggt aagccaaatg gcaaatatga aaggtattga
     4861 agcttctatt gttgtagata taaatttgga gttggctcat aaagctttta ctgatgcagg
     4921 aatagatgaa gaaattatag ataatgtaac aaatgaaaag gatgcagaaa taaaattaca
     4981 agaaggaaaa gtattgacat gtaatgactt tcttgtggct tgtaaaacta agtcaataga
     5041 tgtagttata gatgcaactg ggggtattgc aattggtgca gagatagcat taaactctat
     5101 tttaaataaa aaacatatag ttatgttaaa tgctgaaact gattgtgttg ttggaccaat
     5161 acttaaaaaa ttagcagatg atgctggagt gatatttaca ggttctgcag gagatgagcc
     5221 aggtgcagta atggaattat ttgattttgc agatgcaatg gggtttgaag ttagagtgat
     5281 aggtaaaggt aaaaataata aattagattt agattgtaat cctgatacag ttagagaaga
     5341 agcagaaaga aaaggagctt cacctcatat gatagcttct tttaaagaag gaactaaaac
     5401 aatggtagaa atggctttaa tgtgtaatgc aactggattt gtgcctgatg taagaggtgg
     5461 tcatggtatt gaagctacag ttaatgaagt acctaagaaa tatgcattaa aaagcgaagg
     5521 tggagtatta gataattatg gtgtagttga ttttgtaaat ggtatagcac ctggagtttt
     5581 cgtagtagta gcccataaat taaaagcagt taatgatgaa ctaaaatatt taagtatggg
     5641 tgatggacct aattacatat tatatagacc atatcattta tgtagtttag agactccttt
     5701 atcagcagct atggcagtct tagaccataa ggctacaata gtacctaaag ctggactagt
     5761 agcagaagtt atgactatag ctaagaaaga tttgaaaaaa ggtgagttta tggatggtta
     5821 tggagcatat acatgctatg gtactataga aaaatacgat gtagcaaagg ctatgaatgc
     5881 tgtacctatt ggtttgataa gtaaaaagac taaagttgta aaagatatta aaaaaggtga
     5941 agttataact tacgatatgg tagagataga aaaagatact actttatatc atttaagaca
     6001 attgcaagaa aaaatatttg ggtaagattt gatggtaata taaatagata ttagtaaaaa
     6061 gggttgatgg ttatttttaa atcaaccctt ttaaattttt atatattctt gttaggatac
     6121 tttctttaaa agtatattat aggttatttg tagtcgtaaa aattattttg aattagttct
     6181 gcaattttat attcacattg aaacaatatt attaaaaatt ttaatataaa atgaattaaa
     6241 a
//
LOCUS       DAMJWP010000204         6116 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.211, whole genome shotgun sequence.
ACCESSION   DAMJWP010000204 DAMJWP010000000
VERSION     DAMJWP010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6116
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.211"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            141..3320
                     /locus_tag="PC700_003066"
     CDS             141..3320
                     /locus_tag="PC700_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2582341.1"
                     /translation="MKIDMIYKLIRRNTEPSRWSRGVKYYTNQLVDYIDIDIDDLQNN
                     LLTFRGGVNSEDNYSFYFNSITIDIKHFKIIENDCSCADCQSKGKSIFICKHIVAIVI
                     KSLKQIKNKRDVPPILIDILGADKDKLSNTNSKTSLDLSKQLLSLLDNTKRERVNLDV
                     LINVDSNKAIAEFKIGIDKMYILKNITEFAYSKLNGYELDFGKKFTYNPNIHYFADED
                     EKLVDIIEEYGTNLSYNFSSSHSKKFMEINASSLKQFLNAIKYKDFTLIFSKEEYHPK
                     ILNDTLPVRFDFKNIEDKIVLYSKDSLPVPLTKKNDVLLYDGNIYLLSHKKAHDYSKI
                     YQILSKTKELKFDKEDSKDVLGLLVPRLKSISQEVHLDDNIKNNITKDFKSEFYFDMI
                     DGNICCDVKYIYDDEDKKFVLPNIQKETTIENKLTSSQFTKENNHYVFKGTDYDLFTF
                     LDLQLESLKEFGDIYYSEKFKLKKIYNASSIQASINQTNQNYLEFNFNISDINPKEYK
                     DILKAFQEKRTFYKLKDGNFIDLSERETKDFFELVENLDIMDKSKNNKIYKNKALYIN
                     DVIRSKNLKFIDGKKELDSICEKFRNFDSINLEIPANLNANLRDYQINGLNWFKVLDY
                     YKFGGILADEMGLGKTIQTIAFLLSLSNKKSLIVTPTSLIYNWKNEFEKFAPDIKVLL
                     IHGNKRDREKCFMELENFDVILTTYGTLRNDLEKYSEIKFDYCILDEAQNIKNPVALV
                     TESVKSINAENKFALTGTPMENNLLELWSIFDFIMPGYLYSKAKFQELFINKEDNVKN
                     LKKLIKPFILRRSKKQVMKELPDKIEKNFFVELNKEQKKIYSVYSKDIQDKMKDKNLK
                     KDKIVIFSYLTKLRQLCLDPSIVVKDYNKKSSKIETCLEILRDSINENHKILLFSQFT
                     SVLKNISKELDKYKIKYHYIDGKTNAKERLELVDEFNNSMDKKVFLISLKAGGTGLNL
                     TSADMVIHFDPWWNPSVENQASDRAHRFGQKNSVQVIKLIAKGTIEEKIIKLQESKKE
                     LINQFINGELSNEGVLKSLSDEEIIDLFN"
     gene            complement(3501..3584)
                     /locus_tag="PC700_003067"
                     /pseudo
     CDS             complement(3501..3584)
                     /locus_tag="PC700_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009005413.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            complement(3605..4369)
                     /locus_tag="PC700_003068"
     CDS             complement(3605..4369)
                     /locus_tag="PC700_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2582342.1"
                     /translation="MAYFNYNDKQCYYNEIGDGTPLLFLHGNTASSKMFNDIIDFYKD
                     EYKVILIDFVGHGKSQMVDKFSADLWFDEAMQVICFLEAMNYKKVNIIGSSGGALVAL
                     NVALERPDLVNKVIADSFEGEVPLESFVQNVKIEREASKQDDGAKAFYIYNQGENWEK
                     VVDNDTEAIFEHYKTIGKFFHKPLETMQPEVLLTGSREDEFVSLISSDFFENTFSLLL
                     EKIKNGKMYLFDKGGHPAILSNGLDFSNVAKKFLEE"
     gene            complement(4606..6099)
                     /locus_tag="PC700_003069"
     CDS             complement(4606..6099)
                     /locus_tag="PC700_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2582343.1"
                     /translation="MKYTFEKFKLNEKILKSLKSLGYNIPSRVQREVIPKLLKGQNLV
                     VRSKTGSGKTVSFAIPLCENINVDCNNIQALIVVPTRELALQVKDEISDIGRLKKVRC
                     SAIFGKQSIKDQIAELKQRVHIVVATPGRILDHINKGSIKLENVKYLVIDEADKMFNK
                     GFVEQMEKILLNLPKEKIVSLFSATIDEEIKYICEKYMLDYSVINIEENESDTNQKTR
                     QIDDKIIKANGREKYILLKELIYSENPKSVIIFCNTKEKVSKLYNKMSKEGFLIRELH
                     ADLSQERRIFVIKDFKNQKFNILVSSDVASRGIHIDDISLVINYDVPQDKENYIHRIG
                     RTGRKGNSGKAITIVTEKDEKYIENIETYIGYKVNELKDIEKSRITHGKILFEEYSKG
                     ILKKVSKRKKVDKNSYKSKDIKLNIESEVVKLYLNAGKKKKIRVLDIVGAFSNIKGIT
                     NDDIGVIEVQDLCSYVDILNYKGDLILKKYKEIPIKKKMVKVKRDIR"
BASE COUNT         2291 a          840 c          715 g         2270 t
ORIGIN      
        1 atctaatagt aaattgttat ttacacatat attgtaatag ataaaagcta ttcatatctg
       61 ccttaatttt agtcttaaat ttagagtgta aattgaatgc atttagatta taatatatac
      121 agtgaggtaa ggtgaataat atgaaaatag atatgatata taaactaata agaagaaata
      181 cagaaccatc aagatggtca agaggtgtaa aatattatac taatcaatta gttgattata
      241 tagacataga tattgatgac ctacaaaata acctgttgac atttcgtggt ggagtcaatt
      301 ctgaagataa ttatagcttt tattttaatt caataactat agatataaag cactttaaga
      361 taattgaaaa tgattgtagt tgcgctgact gtcaatcaaa gggcaaaagt atttttatat
      421 gtaagcatat agttgctatt gtgataaaaa gcttaaaaca aataaaaaat aaaagagatg
      481 ttcctcctat tctaatagat atattaggtg cagataagga taagctttct aatacaaatt
      541 ctaaaacaag cttagattta agcaagcaat tactttcttt gttagacaat actaaaagag
      601 aaagggttaa tttagatgta ttgataaatg tggattcaaa taaagcaatt gctgaattta
      661 aaataggaat tgataaaatg tacattttaa aaaacattac tgaatttgca tattcaaaac
      721 ttaatggata tgaattagat tttgggaaga aatttactta caaccccaac attcattatt
      781 ttgctgatga agatgagaag ttagtagata ttatagaaga atatgggaca aatctttcat
      841 acaacttttc ttcatcacat tcaaaaaaat ttatggagat aaatgcaagc tctttaaaac
      901 aatttttaaa tgctattaag tacaaggatt ttacccttat tttttctaaa gaggaatatc
      961 atccaaagat attaaatgat actctacctg taaggtttga ttttaaaaat atagaagata
     1021 aaatagtctt atattctaaa gattcactac ctgttccact tactaaaaaa aatgatgtac
     1081 ttttatatga tggcaatatc tatttattat cacataaaaa agctcatgat tattcaaaaa
     1141 tatatcaaat attatcaaag actaaagagt taaaatttga taaagaggat tctaaggatg
     1201 ttttaggatt gctagttcct agattaaaat ctatatctca agaagttcat ttagatgata
     1261 acataaaaaa taatataaca aaagatttta aatctgagtt ctactttgat atgattgatg
     1321 gcaacatatg ttgtgatgta aagtatatat atgatgatga ggataaaaaa tttgtgctcc
     1381 ccaacataca aaaggaaact actattgaaa ataaacttac ttcttcacaa tttacaaagg
     1441 aaaataatca ttatgtgttt aaaggaactg actatgacct atttacattt ttagatttac
     1501 aattagaatc tctaaaggaa tttggagata tatattattc tgagaagttt aaattgaaaa
     1561 aaatatataa cgcttcatca attcaagctt caatcaatca gactaaccag aattacctag
     1621 aatttaattt taatatatct gatataaatc caaaagagta caaggatatt ttgaaagctt
     1681 ttcaagaaaa acgtactttt tataaactta aagatggaaa ctttatagat ttatcagaaa
     1741 gagaaacaaa agattttttt gaattagttg aaaatctaga tataatggat aaatcaaaaa
     1801 ataataaaat ttataaaaat aaagctctat atataaatga tgtaatacgt agtaaaaatt
     1861 taaagtttat agatggtaaa aaagaactag acagtatatg tgaaaaattt agaaattttg
     1921 atagcataaa tttagaaatt cccgcaaatt taaatgcaaa ccttcgtgat tatcaaataa
     1981 atggcctcaa ttggtttaaa gttttagatt attataaatt tggtggtata ttagctgatg
     2041 aaatgggtct tggtaaaaca atacagacta ttgcattttt attatcatta tctaataaaa
     2101 aaagtcttat tgttacaccg acatcactca tttataattg gaaaaatgaa tttgaaaagt
     2161 ttgctccaga tataaaagtt ttattgatac atgggaataa aagagataga gaaaaatgtt
     2221 ttatggaatt agaaaatttt gatgtaatac ttacaacata tggtactctt agaaatgatt
     2281 tagaaaaata tagtgaaata aagtttgatt attgtatatt agatgaagct caaaatataa
     2341 aaaatccagt agctttagta actgagtctg taaaaagtat aaatgcagaa aataaatttg
     2401 cactcactgg tacacctatg gaaaataatc tactagaatt atggtctata tttgatttta
     2461 taatgcctgg ctatctatat agtaaagcaa agtttcagga attgtttata aataaagagg
     2521 ataatgttaa aaacctgaaa aaattaataa aaccttttat tcttagaaga agtaaaaaac
     2581 aagttatgaa agaacttcca gataagattg aaaaaaactt ttttgttgaa ttgaataaag
     2641 agcagaaaaa gatatatagt gtatattcaa aagatataca agataaaatg aaagataaaa
     2701 atctaaagaa agataaaata gttatatttt cttatttaac taaactaaga caactttgtt
     2761 tagacccaag tatagtagta aaagattaca ataaaaaaag ttcaaaaatt gaaacatgtt
     2821 tagaaatact tagggatagt ataaacgaaa accacaaaat attattgttt tcacaattta
     2881 cttctgtgct taagaacata agtaaggaac ttgataagta taaaattaaa taccattata
     2941 tagatggtaa aactaatgcc aaagaaagac ttgaacttgt agatgagttc aataatagta
     3001 tggataaaaa agttttttta atatctttaa aagcaggcgg aactggttta aatcttacat
     3061 ctgctgacat ggtaattcac tttgacccat ggtggaatcc atcagtagaa aatcaagcta
     3121 gtgatagagc tcatagattt ggtcaaaaaa attcagtaca agttataaaa ttgattgcta
     3181 aagggactat agaagaaaaa ataataaaat tacaagaaag taaaaaagag cttataaatc
     3241 aatttataaa tggagaactt tctaatgaag gtgtattaaa aagtctatct gatgaagaga
     3301 taatagactt atttaactaa attatttaat cctacagctt aactatataa atttattcta
     3361 acatttatat agttaagcta caatatctga ataaataaaa tttcatattg ttatttaatc
     3421 ccaactttac aatctaatga tatctctttc tatacatatt gcatatacaa atgtaataac
     3481 ttgactatta gaatattttg ttttaacaat cctatttcat cataataatg aagtatatgt
     3541 acactcactt ttgtaagttt tgcaatccat tttacagttt tcattattgt aaattttggt
     3601 taaattattc ctctaaaaat ttctttgcta catttgaaaa atctaatcca ttcgataaaa
     3661 ttgcaggatg accaccttta tcaaacaaat acattttacc atttttaatc ttttcaagaa
     3721 gtaatgaaaa agtattttca aaaaaatcac tagatattag agatacaaac tcatcttctc
     3781 tacttcctgt taaaagcacc tctggttgca tagtttctag aggcttatga aaaaattttc
     3841 ctatagtttt ataatgttca aaaatagctt ctgtatcatt atctacaact ttttcccagt
     3901 tttctccttg attataaata taaaaagctt ttgcaccatc atcttgcttt gatgcctcac
     3961 gctctatttt aacattttgt acaaatgact ctaaaggaac ttctccttca aaactatctg
     4021 caattacctt attcactaaa tcaggtctct caagtgcaac atttaatgca acaagagctc
     4081 ctccactact accaattatg tttacttttt tatagttcat tgcttctaaa aaacatatca
     4141 cttgcatagc ctcatcaaac cataagtcag cagaaaattt atcgaccatt tgagattttc
     4201 catgacctac aaaatcaatt aatattactt tatattcatc tttataaaaa tcgattatat
     4261 cattaaacat tttggaagag gctgtatttc catgtaaaaa aagtaatggt gtaccatccc
     4321 caatttcatt atagtagcac tgtttgtcgt tatagttaaa atatgccata tattacctcc
     4381 tttaatatcc ctataggtat atatttatta cttatattta aaatgactat tgtcataaac
     4441 atttaaaaat agtatagctt agaatgtaga taaagtcaac attcctttcc ttaacaactt
     4501 ctattgtttt ttgaaacttc atttcgataa cagaataatt cataaatatt gatactctta
     4561 cttaaatata tgatttcatc atcagagaga gcagtaataa ctaaattatc gtatatctct
     4621 tttcacttta accatttttt tctttatagg tatctcctta tattttttta atatcaaatc
     4681 tcccttataa tttaatatat ctacatatga acataaatct tgaacttcaa ttacacctat
     4741 atcatcatta gttatacctt ttatattact aaatgcacca actatatcta aaactcttat
     4801 tttcttcttc ttaccagcat ttaaatataa tttaacaact tcactttcta tatttagttt
     4861 tatatcttta cttttgtaag aatttttatc aacttttttt ctctttgata ctttttttag
     4921 gattcctttt gaatattctt caaacaaaat tttgccatgt gtaattctac ttttttctat
     4981 atcttttaat tcatttactt tatatcctat atacgtttct atattctcta tatatttttc
     5041 atccttttca gttactatag taattgcctt tccactattt ccttttctcc cagttctacc
     5101 tattctatgt atatagtttt ctttatcttg tggcacatca taatttatta ctaaagaaat
     5161 atcatcaata tgtattcctc tggacgctac atcactacta actaatatat taaatttttg
     5221 gtttttaaaa tccttaatta caaaaattct tctttcttgt gataaatctg catgtaactc
     5281 tcttattaaa aaaccctctt tactcatctt attatataat ttagatactt tctctttggt
     5341 attacaaaat attatcacac tctttggatt ttcgctgtat attaattctt ttaaaaggat
     5401 gtacttctct cttccatttg ccttgattat tttgtcatct atttgtcttg ttttttgatt
     5461 agtatcgctc tcattttctt ctatatttat aacactgtaa tctagcatat atttttcaca
     5521 tatatatttt atctcttcat ctattgtagc tgaaaaaaga cttactattt tctccttagg
     5581 taaatttaat aatatttttt ccatttgttc aacaaaacct ttattaaaca ttttatctgc
     5641 ctcatctatt actaaatact ttacattttc caacttaata gaacctttat ttatatggtc
     5701 aagtattctg cctggagtag ctacaacaat atgaaccctt tgtttcaatt cagctatttg
     5761 gtcttttata gattgtttgc caaatatagc actacatctc accttcttta gtctccctat
     5821 atctgatatc tcatctttta cttgcaaggc taattctctt gttggaacca ctataagagc
     5881 ttgaatatta ttgcaatcta catttatatt ctcgcataaa ggtattgcaa aacttactgt
     5941 ctttccactt cctgtttttg acctaactac taagttttga ccttttaata attttggaat
     6001 aacttctctt tgtactctag aaggtatatt atatcctaaa ctcttcaatg acttaagtat
     6061 cttttcattt aacttaaatt tttcaaatgt atatttcata tattaccacc ttatta
//
LOCUS       DAMJWP010000205         6061 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.212, whole genome shotgun sequence.
ACCESSION   DAMJWP010000205 DAMJWP010000000
VERSION     DAMJWP010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6061
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.212"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(79..2355)
                     /gene="slpA"
                     /locus_tag="PC700_003070"
     CDS             complement(79..2355)
                     /gene="slpA"
                     /locus_tag="PC700_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein SlpA"
                     /protein_id="HDF2582344.1"
                     /translation="MNKKNIAIAMSGLTVLASAAPVFAAEDMSKVETGDQGYTVVQSK
                     YKKAVEQLQKGLLDGSITEIKIFFEGTLASTIKVGAELSAEDASKLLFTQVDNKLDNL
                     GDGDYVDFLISSPAEGDKVTTSKLVALKNLTGGTSAIKVATSSIIGEVENAGTPGAKN
                     TAPSSAAVMSMSDVFDTAFTDSTETAVKLTIKDAMKTKKFGLVDGTTYSTGLQFADGK
                     TEKIVKLGDSDTINLAKELIITPASANDQAATIEFAKPTTQSGSPVITKLRILNAKEE
                     TIDIDASSSKTAQDLAKKYVFNKTDLNTLYRVLNGDEADTNRLVEEVSGKYQVVLYPE
                     GKRVTTKSAAKASIADENSPVKLTLKSDKKKDLKDYVDDLRTYNNGYSNAIEVAGEDR
                     IETAIALSQKYYNSDDENAIFRDSVDNVVLVGGNAIVDGLVASPLASEKKAPLLLTSK
                     DKLDSSVKAEIKRVMNIKSTTGINTSKKVYLAGGVNSISKEVENELKDMGLKVTRLAG
                     DDRYETSLKIADEVGLDNDKAFVVGGTGLADAMSIAPVASQLRNANGKMDLADGDATP
                     IVVVDGKAKTINDDVKDFLDDSQVDIIGGENSVSKDVENAIDDATGKSPDRYSGDDRQ
                     ATNAKVIKESSYYQDNLNNDKKVVNFFVAKDGSTKEDQLVDALAAAPVAANFGVTLNS
                     DGKPVDKDGKVLTGSDNDKNKLVSPAPIVLATDSLSSDQSVSISKVLDKDNGENLVQV
                     GKGIATSVINKLKDLLSM"
     gene            complement(2581..4170)
                     /locus_tag="PC700_003071"
     CDS             complement(2581..4170)
                     /locus_tag="PC700_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp12"
                     /protein_id="HDF2582345.1"
                     /translation="MKVNKRVLSIGLTISLIMAGAPNINALSSIEKIQGKDRYETAAK
                     IAQKQTYENVVLVNTDNTLADGLSASGLAGTVKAPILLSQRNSIPSDTEKMLKDVKKV
                     YIIGTEDSIGKSVENELKQKGIEVKRIGGNDRIETSYLIAKEIASIKPIDKVFITNGY
                     TGEADAMSASSVASRDGSPIILTNGKNVPFEKKEGVQCYALGSEEIISNDLVKKTNAV
                     RLAGEDRFETNKKVIKHFYSSAKEFYLSKGYQLVDAVAGSSIAKNAPIVLVDGNSDKS
                     VLRSADKITALGGIDEKTLEQCLSASSLDASAPTITVGNLNIYQGDKFDISKLNIVAK
                     DSNGNDLTPELIGNIDTDKVGKYKVTIKATDIGGKTTSISVEVNVLEYKTNDMNSSEF
                     KRMVSSEMYSLVNSYRKEKGKEPLQVSKNLEGLSNSWSKYMADKGQFSHVINGKKSVE
                     VFTGYGLRSEENIAFVPLVTKSTYTTKDAREVANVIFTVWKKSDKYNENMLNSDFVYT
                     GFGLYILPNGEVYATQEFLNK"
     gene            complement(4389..5990)
                     /locus_tag="PC700_003072"
     CDS             complement(4389..5990)
                     /locus_tag="PC700_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp11"
                     /protein_id="HDF2582346.1"
                     /translation="MKMNKKILSLGLAVSLILVNFKSVNASSVVEKIYGKDRYETAAK
                     IADRQTYETVILVNTEKSLADGLSASGLSGATKAPILFTQQNKIPADTNRCLKNIKKA
                     YIIGTEDTISKSVEKELDSKNIEVKRIGGEDRLKTSYLIAKEIATIKKVDKVLLTNAY
                     SGEADAMSVSSVATRDGAPIILTDGKSVPFDVKNIQSYCIGSEEIMSNPLVKNTNSVR
                     IEGTDRFETNKNVIDYFFNSADGFYVSDGYQLVDAIAAAPLTKNSPMVLVNDGSDKTV
                     LEGAKNITSVGEINEKVIQQCINASKSNGQPPTITVGSTEIYKGEKFDTGKLNIVAKD
                     NTGKVLPIEVDGFIDTNRVGTYILTLKATDEWGKSTGKRVEIKVLDDKSYDYNSPEFK
                     KMVSTEMYNLINSYRKEKGKEPLVVSSRLEGMANAWSKYMMDKKVFAHYIDGKNAPQV
                     FSEFGMRSEENIAYIYIDSKNVQTTQDAKNLAKAIFEVWKKSPEYNANMLSDEFYSTG
                     FGLYILSDGQVHATQEFLNGNEGSL"
BASE COUNT         1740 a         1179 c          659 g         2483 t
ORIGIN      
        1 gtctcttttt ttgtattcga aaacataata taaaaagact tctcatataa gaagcctttt
       61 tatattatta tatttatatt acatacttaa taaatctttt aatttattta taactgaagt
      121 agctatacct ttaccaactt gaactaagtt ttctccatta tctttatcaa gaactttact
      181 tatagataca ctttgatctg aagataaaga atcagtagct aatactatag gtgctggaga
      241 tactaattta tttttatcat tatcagaacc agttaatact ttaccatctt tatctactgg
      301 cttaccatca gaattaagag ttacaccaaa gtttgctgca actggagctg ctgctaaagc
      361 atcaactaat tgatcttctt tagtagaacc atctttagct acaaagaaat taactacttt
      421 tttatcatta tttaagttat cttgataata agaagattct tttataactt ttgcattagt
      481 tgcttgtcta tcatctccac tatatctatc tggagattta cctgtagcat catctattgc
      541 attttcaaca tctttagata cactgttttc tccacctatt atatcaactt gtgaatcatc
      601 taagaaatct tttacatcat catttatagt tttagctttt ccatctacaa ctactattgg
      661 tgtagcatca ccatcagcta aatccatttt accattagca tttcttaatt gagatgcaac
      721 tggagctata ctcatggcat ctgctaatcc tgttcctcca actacaaatg ctttatcatt
      781 atcaagacct acttcatcag ctatttttag agaagtttca tatctatcat ctcctgctaa
      841 tcttgtaact ttaagtccca tatcttttaa ttcattttct acttctttag atatagaatt
      901 aactccacca gctaaataaa ctttctttga agtatttata cctgttgtac tctttatatt
      961 cataactctc tttatttcag cttttacgct tgaatctaat ttatcttttg aagttaataa
     1021 taaaggagct ttcttttcag aagctaaagg agaagctaca agtccatcaa ctattgcatt
     1081 tcctccaacc aatactacat tatcaactga atctctaaat atagcatttt catcatcaga
     1141 gttataatat ttttgactta atgctattgc agtttctatt ctatcttctc ctgctacttc
     1201 tatagcattt gaatatccat tattatatgt tcttaaatca tccacataat cttttaagtc
     1261 tttcttctta tctgacttaa gagttaattt aactggtgaa ttttcatcag caattgaagc
     1321 ctttgcagca ctcttagttg taactctttt tccttctgga taaagaacca cttgatattt
     1381 tccactaact tcttctacta atctattagt atctgcttca tccccattta atactctgta
     1441 aagagtattt aaatctgttt tattaaatac atatttttta gctaaatctt gtgctgtttt
     1501 actagaacta gcatcaatat ctattgtctc ttcttttgca ttcaatattc taagtttagt
     1561 tattactggg cttccagatt gtgttgttgg tttagcaaac tcaatagtcg cagcttgatc
     1621 atttgcactt gcaggtgtta ttattaattc tttggctaaa tttatagtat cactatctcc
     1681 taatttaaca attttttctg tttttccatc tgcaaattga agacctgttg aataagtagt
     1741 tccatcaact aaaccaaact ttttagtttt catagcatct tttatagtaa gtttcacagc
     1801 agtttcagtt gaatctgtaa aagctgtatc aaatacatct gacatagaca taactgcagc
     1861 actacttgga gctgtatttt ttgctcccgg agtaccagca ttttcgactt caccaataat
     1921 actacttgta gctactttta ttgcactagt tccacctgtt aaatttttta atgcaacaag
     1981 tttacttgta gttactttat ctccctctgc tggagagctt attaagaaat ctacataatc
     2041 cccatcacct aaattgtcta atttattatc tacttgtgta aacaataatt tacttgcatc
     2101 ttctgcacta agctcagctc ctacttttat agtagatgct aaagttccct caaagaaaat
     2161 tttaatctct gttatacttc catctaataa ccctttttgt aattgttcaa ctgctttctt
     2221 atacttgctc tgtactactg tatatccttg atcaccagtc tcaactttcg acatatcttc
     2281 tgctgcaaac acaggtgctg cagaagctaa tactgttaat cctgacatag ctattgctat
     2341 attcttctta ttcatttctt aaattcctcc caacattata ttaaaattac atctcttcat
     2401 tatgtacata gaataatcat ccatatattc ctaatatttc tcacatctct ataataatcc
     2461 atttttattc tttttgcaag tcattttttt gaaattatta tttttcccaa gcatatgagt
     2521 ttttataccc ttaaactcca atatttaata acttaaaaaa tttgatttta cttatttaaa
     2581 ttacttattt aaaaattctt gtgtagcata cacttctcca ttaggcaata tatataatcc
     2641 aaacccagta tatacaaaat cactatttaa catattttca ttatatttat ctgacttttt
     2701 ccaaactgta aatattacat ttgctacttc tctagcatcc tttgttgtat aagtactttt
     2761 tgtaacaagt ggtacaaaag ctatattttc ttcacttctc aagccatatc cagtaaatac
     2821 ttcaacagat ttcttaccat ttattacatg tgaaaactga cctttatctg ccatgtactt
     2881 agaccaggaa ttagaaagtc cttctaagtt tttagaaact tgtaatggct ctttaccttt
     2941 ttcttttcta tatgaattta ccaaactata catttcacta gaaaccattc ttttaaactc
     3001 actactattc atatcattag tcttatattc aagtacattt acttctacac ttattgatgt
     3061 agtctttcca ccaatatcag tagcttttat agtaactttg tatttaccca ccttatcagt
     3121 atcaatattt cctataagct ctggtgtcaa atcgttacca ttgctatcct ttgctacgat
     3181 attcagttta cttatatcaa acttatcacc ttgataaata tttaaatttc caacagttat
     3241 tgttggtgca cttgcatcca gtgatgatgc acttaaacat tgttctagtg tcttttcatc
     3301 tattcctcct agtgctgtaa tcttatcagc acttctaagt acagacttat cactatttcc
     3361 gtcaactaat acaataggtg cattttttgc tatagaagaa ccagcaactg catctactaa
     3421 ttgataacct ttagaaagat aaaattcttt tgccgaacta taaaaatgtt ttataacttt
     3481 tttattagtc tcaaatctat cttctccagc taatctaact gcattagttt ttttaactaa
     3541 atcattactt attatctcct ctgaaccaag agcataacat tgcacacctt cttttttctc
     3601 aaatggaaca tttttaccat tagttaaaat tataggtgat ccatctcttg atgctactga
     3661 tgacgcactc atagcatctg cttcacctgt ataaccatta gttataaaaa ccttatctat
     3721 aggttttatt gaagctattt cttttgctat aagataactt gtttcaattc tatcattacc
     3781 acctattctt ttaacttcta ttcctttttg ctttaattca ttttcaactg attttcctat
     3841 tgaatcttca gttcctatta tataaacctt ctttacatct tttaacattt tttctgtgtc
     3901 tgatggtata ctgtttcttt gtgataataa tataggtgct ttaactgttc ctgctaatcc
     3961 acttgcactt aatccatcag ctagtgtatt atctgtattt actaatacta cattttcata
     4021 tgtttgtttc tgtgcaattt tggcagctgt ctcatatcta tctttccctt gtatcttttc
     4081 tatgctcgaa agtgcattga tatttggtgc tcctgccatt attaatgata ttgtcaatcc
     4141 tattgataat actctcttat ttaccttcat cgaactcctc ctactaattt ttttatataa
     4201 ttcagctaat cataaattac atcctcagta gtgattccct caaaatctat aaactactaa
     4261 aacaaaaaac acctaaaata ccatagcctg taaagcaata acaaaacaga ttacaaaaag
     4321 tactttagat gtgattttca ttttataata tcagtaaatt aacaaaaaaa caagatttat
     4381 ggaatttttt acaaactacc ttcattgcca tttaaaaact cctgagtagc atgcacttga
     4441 ccatcagata atatataaag cccaaatcca gtactataga actcatcact aagcatgttt
     4501 gcattgtact ctggagactt cttccacact tcaaatatag cctttgccaa gttttttgca
     4561 tcttgtgtcg tttgaacgtt cttagaatct atataaatat atgctatatt ttcctcactt
     4621 ctcattccaa actcagaaaa tacttgtgga gcattttttc catctatata atgtgcaaat
     4681 acttttttat ccatcatata cttagaccat gcatttgcca taccttctag cctactagat
     4741 actaccaaag gctctttgcc tttttctttt ctataggaat tgattaagtt atacatttca
     4801 gtagatacca tttttttaaa ttctgggctg ttgtaatcat aggatttatc atccaacact
     4861 tttatttcta ctctttttcc tgtacttttt ccccattcgt ctgtagcttt taatgtcaat
     4921 atgtatgtac ctactctatt tgtatctata aacccatcaa cttctattgg taataccttc
     4981 ccagtgttat ctttagccac tatgtttaac ttgccagtgt caaacttttc acccttatat
     5041 atctctgtac ttccaactgt aattgttggt ggttgtccat ttgactttga agcatttata
     5101 cactgttgta ttaccttctc atttatttca cctacagaag ttatattctt agctccttct
     5161 aatacagttt tatcgcttcc atcatttact agtaccattg gagagttttt agtaagtggt
     5221 gcagctgcta ttgcatctac taattggtat ccatctgata catagaatcc atctgcacta
     5281 ttaaaaaagt agtctattac atttttgtta gtttcaaatc ggtcagttcc ttctattctt
     5341 actgagtttg tatttttaac tagagggtta ctcattatct cctctgaacc tatacaatat
     5401 gattgaatat tttttacatc aaaaggcaca ctctttccat ctgtaagtat gattggagct
     5461 ccatctctag tagctactga tgatacactc attgcgtctg cttctccact atatgcgtta
     5521 gttaatagta ctttatccac tttttttata gttgctattt ctttagctat taaatagctt
     5581 gtttttagtc tatcttctcc accaattctt ttgacttcta tatttttaga atctagttct
     5641 ttttctactg atttacttat agtatcttct gtacctatta tatatgcttt tttgatattt
     5701 tttagacatc tatttgtgtc tgctggtatc ttattttgtt gtgtaaatag tattggagct
     5761 tttgtagctc ccgataatcc acttgcactt aatccatctg caagtgattt ttctgtattt
     5821 actaaaatta ctgtttcata agtctgtcta tcagctatct ttgctgctgt ttcatatcta
     5881 tctttaccgt atattttttc tactactgat gatgcattta cacttttgaa gtttactaag
     5941 attaatgata ctgctagacc taatgataat atttttttgt tcattttcat atccccctga
     6001 tgatttttat attttatatt tatattgtga ttattttcta tttattttct ctttattttc
     6061 c
//
LOCUS       DAMJWP010000206         6044 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.213, whole genome shotgun sequence.
ACCESSION   DAMJWP010000206 DAMJWP010000000
VERSION     DAMJWP010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6044
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.213"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            29..679
                     /locus_tag="PC700_003073"
     CDS             29..679
                     /locus_tag="PC700_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HDF2582347.1"
                     /translation="MKAEKTSIYPIIKNNLNKISIVEYKKGQRFVASDKDLQEVFFII
                     EGVALVECTTRGGNKFLVDIVPENEFIGKISYIYEHNLKCDIFAKTNIKLFRFEKNVF
                     EEFYLKPDFIALFHRKCTRRIYELYKTRMVRELFSCTEVIAYCISNKQENNVCNIKAI
                     RNFSEIYSISRKSRYHSLLKLTEKEIVKKMGNTYEILNYDELHKLAFEVKEFLEDE"
     gene            complement(819..2195)
                     /locus_tag="PC700_003074"
     CDS             complement(819..2195)
                     /locus_tag="PC700_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2582348.1"
                     /translation="MQELFSLKDENKRFYKILLSLCIPIIIQNLISTSVNVIDTIMIS
                     SLGETSVASVGVANQFFFLFNMSLSGITGGAGVFISQFYGKKDVSNIRKVTGLTCVLA
                     IVLSFVFVIPALLTPKPIIHIFSYDSEVVKLCIDYFSIAVFSYPLIAVSTVFSTGSRG
                     VRNPKLGMICSAFALVTNVILNYGFIFGNFGLPALGVKGAALATVIARICELILMITY
                     VYLYKKDYILKFGLKNLKAIDKIFIKSFSSKSFPIFVNDSVWAIGTVLYSVAYARAGT
                     SAIAASQIATSTGNFFIMTAVCIASGASIMLGNELGADHIKRAIEYAKKFSILVFSAG
                     LILGIILILNIPLLLKMFSVSDSLASDITKIFFIMGILMALKSFNTLVIIGILRSGGD
                     TKYALFLELGCMWLASIPLTFIAAFKGAPIFVLVLLTYSEEVVKFIFGVPRALSKKWA
                     INIVKEID"
     gene            complement(2211..4124)
                     /locus_tag="PC700_003075"
     CDS             complement(2211..4124)
                     /locus_tag="PC700_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF2582349.1"
                     /translation="MIVLSCNNLNKSFGIDSILENISFTVNEGDKIGIIGVNGTGKTT
                     LFKIISGIYGYDSGDIYTSKDCEIGYLEQNTNFYSDNTILEEVLEVFKNLIEMESYLR
                     ELEVKISEESTKTNSPIIEKIMDEYSHKLELFSDLNGYGYKSEAKGVLKGLGFSDNDM
                     DKPISILSGGEKTRVLLGKLLLKKPTLLLLDEPTNHLDSEAIEWLEVFLKQYKGTVML
                     ISHDRYFLDQSVNRIFEVHNKKLKVYNGNYSKFVELSKIEKELELKKFEDQQKEIKKQ
                     EESIERLKAYGREKHLKRARSKEKALDKVDVLDKPEAYRKKARIQFTPSVQSGNDVLQ
                     IRDVSMGYGERILFKDLDLDIYRGEKVALIGANGVGKSTLFKIITNELQPLSGNIKFG
                     TNVHVSYFHQEQKTLNLDNTIIDEIWENNTHLTQTTLRNMLGAFLFVDEEVFKKISTL
                     SGGERARVAILKLILSNANLLLLDEPTNHLDIDSKEVLEEALTNYDGTIFTISHDRYF
                     LNTVVDKILVLDENGITEYLGNYDYYIDKKRQIQEMSIIEEKEEKTRTQIKDEKRKER
                     EQREIEKKNRIKRQNIEKEIEKLEIEIEKLDILLCQEEVYSNPDKAKEVSQEKINLEN
                     NLASLYDEWEEFM"
     gene            4260..4892
                     /locus_tag="PC700_003076"
     CDS             4260..4892
                     /locus_tag="PC700_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDF2582350.1"
                     /translation="MLGNKNISMAVIRRLPKYHRYLGDLLDRDIQRISSKELSDIIGF
                     TASQIRQDLNNFGGFGQQGYGYNVEALHTEIGKILGLDRPYNAVLVGAGNLGQAIANY
                     AGFRKAGFEIKALFDANPRMIGLKIREFEVLDSDTLEDFIKNNNIDIAVLCIPKNGAQ
                     EVINRVVKAGIKGVWNFAPLDLEVPKGVIVENVNLTESLFTLSYLMKEGK"
     gene            5031..5570
                     /locus_tag="PC700_003077"
     CDS             5031..5570
                     /locus_tag="PC700_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HDF2582351.1"
                     /translation="MVFVEELWKKAIEFHGHECPGLAIGFKAALAARKYLGCKQSGDE
                     EIVCISENDACGIDGIQVVLSCTMGKGNLLLRMTGKSVYHFFNRENGKSIRIYVKSKP
                     FQMDREGYKKYLLESDFEEIFEITDTKLRLPEKARIFKSLKCEVCGEFASEQYIRIEE
                     GKKVCLDCFNKYERFYEEY"
     gene            complement(5746..5916)
                     /locus_tag="PC700_003078"
     CDS             complement(5746..5916)
                     /locus_tag="PC700_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2582352.1"
                     /translation="MAYKITDACISCGACEAECPVSCISAGDDAYVIDAGSCIDCGSC
                     AGACPVDAPQPE"
BASE COUNT         2227 a          856 c          853 g         2108 t
ORIGIN      
        1 acaatatatt gtaaaggagg taactttgat gaaagcagaa aaaacaagta tatatccaat
       61 tattaaaaat aatttaaaca aaatatcaat tgttgaatat aaaaagggtc aaagatttgt
      121 agcatcagat aaagacttac aagaagtgtt ctttataata gagggagttg cattagtgga
      181 gtgtactact agaggaggaa ataaatttct tgttgacatt gtacctgaaa atgaatttat
      241 aggcaaaatc agttacatat atgaacataa tttgaaatgt gatatatttg caaagaccaa
      301 tataaaatta tttcgatttg aaaaaaatgt atttgaagag ttttatctca aaccagattt
      361 tattgcacta tttcacagaa aatgtacaag aagaatatat gaattatata aaaccagaat
      421 ggtaagagaa ctattttctt gcacagaagt tattgcctat tgtatatcaa ataaacaaga
      481 aaataatgtc tgcaatataa aagctattcg taatttcagt gaaatctatt caattagtag
      541 aaaaagtaga taccattctc tgctaaagtt aactgaaaag gagatagtaa aaaaaatggg
      601 taatacatat gaaattttaa actatgatga attacataaa ttggcatttg aagtaaaaga
      661 gtttttggaa gatgagtaaa aattgttact taatcaaaat aaagtataat aatttctgta
      721 aaaaatagaa atagtctcac tgaaaatcaa aaatacttta gttattttca atgagactgt
      781 ttagaaatat tgtatactta taatgtatag tataattatt aatcaatttc ctttactata
      841 ttaatagccc attttttaga taatgctcta ggaacaccaa atataaactt aaccacttct
      901 tcactataag tgagtaaaac gagtacaaat ataggtgcac ctttaaatgc agctataaaa
      961 gttagaggaa tagaagctaa ccacatacat cctaattcta gaaatagagc atatttagta
     1021 tctcctccac ttcttaaaat accaattata accaatgtat taaaagattt aagagccatt
     1081 agtattccca tgataaagaa tatcttagtt atatcagaag ctaagctatc agaaacacta
     1141 aacattttta ataataatgg tatatttaaa attaaaataa ttccaagtat taaaccagct
     1201 gaaaatacaa gtatagaaaa ctttttagca tattcaatgg ctcttttaat atggtcagct
     1261 ccaagttcat ttccaagcat aatagatgca ccagatgcaa tacatacagc tgtcattatg
     1321 aagaaattac ctgtactggt tgctatctgg ctagctgcta tagcagaagt accagctctt
     1381 gcatatgcaa cagaatacag gacagttcca attgcccata cagagtcatt tacaaatata
     1441 ggaaagcttt ttgatgaaaa agattttata aaaattttat caatagcttt tagatttttt
     1501 agaccaaact ttaatatata gtctttttta taaaggtaaa cataagtaat cattaaaatt
     1561 aattcacata ttctagcaat aacagtagct agagcagcac cttttacacc caatgcggga
     1621 agaccgaagt taccaaatat aaatccataa tttaaaataa catttgttac aagtgcaaat
     1681 gcactacaaa tcatacctaa cttaggattt ctaacacctc ttgaaccagt actaaataca
     1741 gtacttacag caattaatgg ataactaaat actgcaatac taaagtaatc tatacaaagt
     1801 tttaccacct cagaatcata cgaaaatatg tgtataattg gctttggtgt tagtaaagca
     1861 ggaataacaa atacaaaact taaaacaatt gccaatacac aagtaagacc tgtgactttt
     1921 cttatattac tcacatcctt ctttccataa aactgtgaaa taaaaactcc agcacctcct
     1981 gtgataccag ataaggacat attaaaaaga aagaaaaatt ggtttgcaac tccaactgaa
     2041 gctacagatg tctcccccaa actacttatc ataattgtat ctattacatt tacagaagta
     2101 gaaattaagt tctgtatgat aataggtata cataaagata gaagtatttt ataaaatctt
     2161 ttattttcgt cttttaaaga aaatagttct tgcaaataat cctctccttt ttacataaat
     2221 tcttcccact catcatatag agaggcaagg ttattttcta aattaatttt ttcttggcta
     2281 acttctttag ctttatctgg attagagtat acttcttctt gacataaaag tatatctaat
     2341 ttttcaattt caatctctaa cttttcaatt tctttttcta tattttgtct ttttattcta
     2401 tttttctttt ctatttctct ttgttctctc tcttttcttt tttcatcttt tatttgagtt
     2461 ctagtttttt cttctttttc ttctataata ctcatctctt ggatttgcct ttttttatct
     2521 atataataat cataatttcc cagatattca gtaattccat tttcatctaa aactaaaatt
     2581 ttatcaacaa cagtatttag aaagtatcta tcatgtgata ttgtaaatat agtcccatca
     2641 taattagtta atgcctcttc aaggacttcc ttagaatcaa tatctaggtg attagtaggc
     2701 tcatctaata acaataagtt tgcatttgat agtataagtt taagtatggc aactctagct
     2761 ctttctccac cactcaatgt agaaatcttc ttaaaaactt cttcatctac aaataaaaat
     2821 gcccctaaca tatttctcaa agtagtttga gtaagatgag tattattttc ccaaatctca
     2881 tcaattattg tgttatctaa attaagagtt ttttgttcct gatggaagta ggaaacatgt
     2941 acattagttc caaatttaat attgccacta agaggttgta gttcattagt tattatttta
     3001 aataatgtag atttgccaac accatttgca cctataagtg caactttttc ccctctatat
     3061 atatctaaat ccaagtcttt aaaaagtatt ctttctccat aacccataga tacatctcta
     3121 atttgaagaa catcattacc actctggaca gaaggcgtaa attgtatacg agcttttttc
     3181 ctataagctt ctggtttatc taaaacgtct accttgtcta atgctttttc tttacttctt
     3241 gcacgcttta ggtgtttttc tctaccataa gcttttagtc tttcaattga ttcttcttgc
     3301 ttttttattt ctttttgttg gtcttcaaat ttcttaagct ctagttcttt ttctatttta
     3361 gataactcaa caaatttaga gtagttacca ttatatactt taagtttttt gttgtgaact
     3421 tcaaaaattc tatttacgga ttggtctaag aaatatctat catgagatat taacatgaca
     3481 gttcctttat attgttttaa aaatacttct aaccattcta ttgcttcaga gtctaagtgg
     3541 ttagtaggtt catcaagtaa caatagagtt ggtttcttaa gaagtaattt tcctaaaaga
     3601 actctagttt tttcaccacc agaaagtatg cttattggct tatccatatc attgtcacta
     3661 aatcctaatc cttttagaac tcctttggct tcagatttat atccatatcc atttaaatct
     3721 gaaaataatt ctagtttatg tgaatattca tccatgattt tttctataat tggagagttt
     3781 gtttttgtac tttcttcaga tattttaacc tccaattctc ttaaatagga ttccatttct
     3841 atcaaatttt taaaaacttc tagcacttct tccaaaatag tattatccga ataaaaattg
     3901 gtattttgtt ctaaatatcc gatttcacaa tctttagaag tataaatatc tccactatca
     3961 tatccataaa ttcctgaaat tattttaaat aaagtagttt ttccagtacc attaacaccg
     4021 attataccaa ttttatcgcc ttcatttact gtaaaactaa tattttctaa tatagaatct
     4081 ataccaaaac ttttatttaa gttattacat gacaaaacaa tcataaattt ctacctcctt
     4141 gaactatcta aaattataat gttttgatat aatataatca aggttatatg tattagtttt
     4201 gaaaaagaaa tattttattg tgaatcaaac aaaaaagagg acacttttag gaggtgaaga
     4261 tgttgggaaa taaaaatata tcaatggcag ttataagaag gctcccaaaa tatcatagat
     4321 atcttggaga cttattagat agggatatac aaagaatatc ttctaaagaa ttgagtgata
     4381 taatagggtt taccgcttct caaataagac aagatttaaa caactttggt ggatttggac
     4441 aacaaggata tggttataat gtagaagctc ttcatactga gataggtaaa attcttgggt
     4501 tggatcgacc atacaacgca gttcttgtag gagcaggtaa cttaggacaa gctatagcca
     4561 attatgcagg atttagaaaa gctggattcg agataaaagc tttatttgat gcaaatccta
     4621 gaatgatagg tttaaagata agagagtttg aagtattaga ttcagatact ttagaagact
     4681 ttataaaaaa caataatata gatattgctg tattatgtat acctaaaaat ggagcacaag
     4741 aagttattaa tagagttgta aaagctggaa tcaaaggtgt atggaatttt gcacctttag
     4801 atttagaagt tccgaaaggt gttatagttg aaaatgtaaa cttaacagaa agtttattta
     4861 ccttatcgta tttaatgaaa gaaggaaagt agacaactta aacatattaa taatgagcag
     4921 ttgtgaagag tattgagaaa gtcagaagat ttaatattaa atcctctggc tttttgaaat
     4981 attaggataa aaatttaaat aatgtatgga aataaacaat attaaggaga gtggtttttg
     5041 tggaggaatt atggaaaaag gcaattgaat ttcatggtca tgaatgtcca gggttagcta
     5101 taggatttaa agcagcactt gcagcaagaa aatatttagg atgtaaacag tcaggagatg
     5161 aggaaattgt atgtatttct gaaaatgatg cttgtggaat agatggtata caagtcgtgc
     5221 taagttgcac aatgggtaaa ggaaatttac tacttaggat gactgggaaa tcagtatatc
     5281 atttctttaa tagggaaaat ggaaaatcta taagaatata tgtaaagtca aagccatttc
     5341 aaatggatag agaaggatac aaaaagtact tattagaaag tgattttgaa gaaatatttg
     5401 agattacaga tactaagtta agacttcctg aaaaagcaag aatatttaaa tctcttaaat
     5461 gtgaagtgtg tggtgaattt gcatctgagc aatatataag gattgaagaa ggaaagaaag
     5521 tatgtcttga ttgctttaat aaatatgaaa gattttatga ggagtattaa aattataaat
     5581 tagattaaag ttaaaagtat tagatatgta ctaacagtat tttaagatga attaggtagc
     5641 aacataataa aatatataaa taatattact tgaaccatac ataattaaaa agaggctgat
     5701 tagtatttag aaaataaaat cagtctcttt atgaataacg aattattatt ctggttgagg
     5761 tgcatctaca ggacaagctc ctgcacaaga accacagtct atacaagaac cagcatctat
     5821 aacatatgcg tcgtctccag ctgatataca gctaacaggg cattcagcct cacaagcacc
     5881 acagcttata caagcgtcag taattttata tgccattttg aaatcctccc ttttatatgt
     5941 attgtaattc aataacatta taacaaatat tattgaatat aacaattaaa ttaattctac
     6001 aacaaaaact ttattatgat attcttgttt taaagtttta caat
//
LOCUS       DAMJWP010000207         6027 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.214, whole genome shotgun sequence.
ACCESSION   DAMJWP010000207 DAMJWP010000000
VERSION     DAMJWP010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6027
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.214"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(214..723)
                     /locus_tag="PC700_003079"
     CDS             complement(214..723)
                     /locus_tag="PC700_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HDF2582353.1"
                     /translation="MSQQNTGNAIVNSKPKKTAVEIFMGGAKKGFYIGVEQILPAMIM
                     GYVIVQFLQLTGLVDILGNIFGPVMGIFGLPGESVVVLIAAFFAKAAGAATAANLFAQ
                     GLITAPQATILIMPCMLMGTLVGHYARIVLVANASTKYRMLLLAVPIFDAIVGMLIVR
                     VLLTVMGLM"
     gene            complement(725..1372)
                     /locus_tag="PC700_003080"
     CDS             complement(725..1372)
                     /locus_tag="PC700_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582354.1"
                     /translation="MDVTAGDNKVTWKGYLSLFVLIVLFSGVFKDSQGFLKALDFANL
                     TGSFGTIADGVDFMGKGGTGAKDGFLFALTLIPTVCFAVGLIDVVESMGGMKAASKIF
                     NPLLRPLLGIPGIAGIAFVSSFTSSDVASIMTKELFESGEITDDERTIFVAYQYAGSA
                     VILNTINTQAPLLPIALLALGPIILIEIFCKILGANLVRLIIGVRNKKNKAKEAV"
     gene            complement(1386..2558)
                     /locus_tag="PC700_003081"
     CDS             complement(1386..2558)
                     /locus_tag="PC700_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2582355.1"
                     /translation="MNTYLNEISKFVDENREEIVSLWKEIVNIESYTHCKESVNKLAE
                     RLKLEFEKEGLDCELVDVGENGNTLIGTLGSNIDKKPIIFSGHMDTVFETGTFGENPF
                     KIIEGKAYGPGVLDMKGGIIISLYVIKALNKIGYKERPIKIVFSGDEEIGHKDSTGAD
                     VILREAKGALCAFNMETGLVDNSLCIGRKGRIGCNIHVKGVETHAGNDFEGGRNAIEE
                     MANKILRIQKLTNLDVGTTVSVSVIKGGRVENSIPEDCSIKVDLRFEKVEEMENVKEQ
                     VREICKETYIEGTSTEVNFISEMMPFETTEDVMRFHTFVNEVSRENGFGELNAKRLGG
                     SSDASYLTIANVPTICSFGVRGEWNHTSREYAVVDSMFERVKLISTVILNLDKFEV"
     gene            2826..4202
                     /locus_tag="PC700_003082"
     CDS             2826..4202
                     /locus_tag="PC700_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582356.1"
                     /translation="MKYKIGFLTTSYFMSDKFKQALKELNDTCEITFIELKGENPFNN
                     LPNIYYEQVDNFDGFVCSGIVPYSELIINIKDIKVPLNFLKLDERDFYKYLFKLLNSQ
                     KDIDFTKSFMDFLREDNNYYDIYSLIDSNKCPYTIKDFNIPQPVYDFKKLNDKLLEIH
                     LDLLRDNKINLSFTRNYIISCELNKLGYDCIYMVPSTESILNTFKSLVNEITLQNLDK
                     NKSATCIVTVNSSEYINEDLIFKNDEIQNQIYNTILNSLSRHGFYGVQVKKNDMKIEI
                     HTTKEILNNMTNNYTDFFISEDLKEIKYSLNIGWGIGNTNIHAEQNARQANGKSASLN
                     GNCTFIVNDTNDTIGPLYSNKNSNNIEDSSIANKVASFIPLSNTNVSKIMCMINDRNS
                     NNVSAEILADYMNITLRSANRILSILYKAGIATIVNTKLDNQRGRPKKIYKVDFLSFL
                     NKMQKLNS"
     gene            complement(4453..5286)
                     /locus_tag="PC700_003083"
     CDS             complement(4453..5286)
                     /locus_tag="PC700_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2582357.1"
                     /translation="MTIKEVEEKTGLTRSNIRFYENENLIKPLRNERNGYREYSDENV
                     EDIKKIAYLRTLGITIDDIRNIILQKVSLVEVVEKQEKVLETKISDLENAKTMCNKML
                     ESDELSYLNLKVEQYITELPEYWNKNKPVFKLDSVSFLYIWGGKITWGIITIACLLIA
                     ITVFPKLPPQIPIQWNNGVANYVDKKFIYAYPVICVVFRILLRPFIWRWLQIHAFYSD
                     TIADYLTNFMCFVALSIEIFSILFIYNIARHVTIIIFIDILVFIVLLIIGVYKLFKKY
                     N"
     gene            complement(5615..>6027)
                     /locus_tag="PC700_003084"
     CDS             complement(5615..>6027)
                     /locus_tag="PC700_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDF2582358.1"
                     /translation="GIDYENYDIVFLGYWVDKGICDKNSKQVLENIHNKKIALFGTMG
                     ASKKGSYGASIIEKIESIIPKDNEILGSFICQGKIAEGLKAKYKEMLKLSPDNEHIRQ
                     QLNNHEESQSHPDEQEIYEASMFAKNMMIKASIV"
BASE COUNT         2102 a         1028 c          646 g         2251 t
ORIGIN      
        1 tttcattgtt gaaattatga aaatatactt gatttaatac caaaagagta tctcatgaac
       61 taaaaagttc attttgagat actctcttct tttttaatta atatacatat taattactat
      121 attagtttat tactatatat caattactat tcattaatta ctattcatca attcttatat
      181 atcatttata attaggttat aattttatgt tatttacatt aatcccataa cagtgagtaa
      241 aactcttact atcaacatcc ccactattgc atcaaatata ggaactgcca acagtaacat
      301 tctatactta gtactggcat ttgctacaag cactattctg gcataatgac caactagagt
      361 acccattaac atacatggca taattaatat ggttgcttgt ggtgcagtga ttagaccttg
      421 tgcaaataag tttgcagctg tagctgctcc tgctgcctta gcaaagaatg ctgctatcaa
      481 aactacaaca gattccccag gtagtccaaa aatacccatt actggaccaa aaatatttcc
      541 taaaatatca actaaaccag ttaattgtaa aaactgcact attacataac ccattatcat
      601 ggctggtaat atctgttcaa cacctatgta aaaacctttt tttgctcccc ccataaatat
      661 ctcaacagca gttttcttag gtttactatt tacaattgca tttccagtat tttgttgact
      721 catactacac agcctcctta gccttattct ttttatttct tacacctatg attaatctaa
      781 ctagattagc accaagtatt ttacagaata tctctataag gattattgga cctaatgcca
      841 ataatgctat tggtaaaagt ggagcttgag tatttattgt gttcagtata acagctgaac
      901 cagcatattg atatgctaca aatatagttc tttcatcatc tgttatttcc ccactttcaa
      961 ataattcttt cgtcattatt gaggctacat ctgaactagt aaatgaagat acaaatgcta
     1021 ttcctgctat tccaggtata ccaagcagag gtctaagtaa tggattaaat atctttgatg
     1081 ctgctttcat tcctcccata gattcaacta catctataag acctacagca aaacagacag
     1141 ttggaatcaa agtcagagca aataagaaac catcttttgc tcccgttcct cctttaccca
     1201 taaagtctac accatctgca atagtaccaa atgaacctgt cagattagca aaatctaatg
     1261 ctttaaggaa gccttgacta tctttaaata ctcctgaaaa taatactatc aatacaaata
     1321 aagataaata ccctttccat gttaccttat tatctccagc tgttacatcc atataaaatc
     1381 cccctttata cctcaaattt atctaagttt aaaataactg tagaaattaa tttcaccctc
     1441 tcaaacatag aatctactac tgcatattct cttgatgtgt ggttccattc tcctcttaca
     1501 ccaaatgaac agattgtagg aacgtttgca atagttaaat aacttgcatc tgaactacct
     1561 ccaagtctct tagcatttaa ttctccaaat ccattttctc ttgacacttc atttacaaat
     1621 gtatgaaatc tcataacatc ttctgtagtt tcaaatggca tcatttcact tataaaatta
     1681 acttctgtag aagttccttc tatgtaagtt tctttacata tctctctaac ttgttcttta
     1741 acattctcca tttcttctac tttttcaaat ctaaggtcta cttttatact acaatcttct
     1801 ggaatagaat tttccactct tccaccctta atcacactta cacttactgt agttccaaca
     1861 tctaaattag tcaatttttg tatgcgtagt atcttattag ccatttcttc tatagcattt
     1921 cttccacctt caaaatcatt acctgcatgt gtttctacac cttttacatg tatattacat
     1981 cctattcttc cttttctacc tatacataaa ctattatcta ctaatccagt ttccatatta
     2041 aaagcacaaa gagcaccttt tgcttccctc aatataacat ctgcaccagt tgaatcttta
     2101 tgacctatct cttcatctcc agaaaatact atttttattg gtctttcttt gtatcctatt
     2161 ttatttaaag ctttaataac gtataaagag attattatac cccccttcat atctaaaaca
     2221 cctgggccat atgcttttcc ttctattatt ttaaatggat tctctccaaa tgtacctgtt
     2281 tcaaagacag tatccatgtg tcctgaaaaa attatcggct ttttatctat attagaacca
     2341 agtgtaccta tcaatgtatt accattttcc ccaacatcta ctagttcaca atccaaacct
     2401 tctttttcaa attcaagctt taatctctca gctaacttgt ttacactttc tttacaatgt
     2461 gtataacttt ctatattaac aatctctttc cataatgaaa ctatttcttc tctattctca
     2521 tcaacaaact tagaaatttc atttaaatat gtattcataa tcttctcctt tattttcaaa
     2581 taaaatgtat ttctttccta aaatctaagt ttattttagg aaatcttaaa agactagtcc
     2641 ttaattacaa aatagcacaa ttattcttgc cagtcaacat atttttcagt cttttttata
     2701 ttttctgatt tttctttcaa ttatcctttt gtaaaataaa ttgacgcgtt ttatctacta
     2761 ttataaaatg aggttataaa ttgattgatt agttacaata ctctcgaaat tttaaaggag
     2821 gttttatgaa atataaaatt ggttttttga caacatctta ttttatgtca gataagttta
     2881 aacaagctct taaagaatta aatgatactt gtgaaattac atttattgag cttaaagggg
     2941 agaatccttt taataattta cctaatatat actatgagca agtagataat tttgatggat
     3001 ttgtatgcag tggtatagtt ccatatagtg aacttataat aaatataaaa gacataaaag
     3061 ttccattaaa ttttttaaag cttgatgaaa gagattttta taaatactta ttcaaattat
     3121 taaatagcca aaaagatatt gactttacaa aatcatttat ggatttttta agagaggaca
     3181 acaattatta tgatatatat tcattaatag actctaataa atgtccatat acaattaaag
     3241 attttaatat ccctcaacct gtatatgact ttaaaaaatt aaatgataaa ttattggaaa
     3301 tacatttaga cttattgcga gataataaga ttaacctttc ttttactaga aattacatca
     3361 ttagttgtga acttaataag cttggatatg attgtattta catggttcca tctacagaat
     3421 caatcctgaa tacatttaaa tctctggtga atgaaataac attacaaaac ttagacaaaa
     3481 ataaatctgc aacttgtata gttacagtaa atagttcaga gtacataaat gaagatttga
     3541 tatttaaaaa tgatgagata caaaatcaaa tatataacac tatattaaat tctctatcaa
     3601 gacatggatt ctatggagtt caggtcaaaa aaaatgatat gaaaatagag atacacacta
     3661 ctaaagaaat attaaacaat atgaccaata actatacaga tttttttatt tcagaagatt
     3721 taaaagaaat taaatatagc ttaaatatag gttggggcat tggaaataca aatattcatg
     3781 cagagcaaaa tgctcgtcag gctaatggta aatctgcttc tttgaatgga aactgtactt
     3841 ttatagtaaa tgatactaat gatacaatag gccctttata ttcaaataaa aattctaata
     3901 atattgaaga tagctcaata gccaataaag ttgcttcttt tatacctcta tctaatacaa
     3961 atgtatctaa aatcatgtgt atgattaatg atagaaattc aaataatgtg tcagcagaaa
     4021 tattagcaga ctatatgaac ataactctta gaagtgctaa tagaatattg agtatcttat
     4081 ataaagcagg aattgcaact attgtaaata caaaactcga taatcaaaga ggaaggccaa
     4141 aaaaaatata taaagttgat ttcttatctt ttctaaataa gatgcagaaa ttaaattctt
     4201 aatatttaat tttttaataa aagcaatgta ttttctctaa ttttaaagaa tcaaaatgtt
     4261 taaattttat tagaatatac aactttttac tttaaaatac ctaaaccttt tatcagtgaa
     4321 ataaaccata atatatcgta gcttattaga aaaacaaaat aatttcatat acaaataata
     4381 aaatagccac tacaatccat aatttgcagt ggctactttt tacactatca cttattactt
     4441 aatttttaaa aattaattgt attttttaaa taatttatac acacctatta tcagtaaaac
     4501 aataaataca agtatatcta taaaaataat tattgtaaca tgtctggcaa tattgtaaat
     4561 aaaaagtata gagaatattt ctatagacaa cgctacaaaa cacataaaat tcgttaaata
     4621 atctgcaata gtatcactat aaaaagcatg tatttgtaac catctccata taaatggacg
     4681 taaaaggatt ctgaatacaa cacaaatgac agggtaggca taaataaatt ttttatctac
     4741 ataattagct acaccattat tccattggat aggaatttga ggtggcaact ttggaaatac
     4801 tgttattgca atcaacaaac aagcaatagt aataattccc caggtaattt tccctcccca
     4861 tatatataag aaacttacag agtcaagttt aaaaacaggt ttgtttttat tccagtattc
     4921 tggcaactca gttatgtatt gctctacttt caaattcaaa taacttagct catcagattc
     4981 taacatttta ttacacatag tttttgcatt ttctaagtct gatattttag tttcgagaac
     5041 tttctcctgt ttctctacaa cttcaactaa agatactttc tgtaatataa tattacggat
     5101 atcatcaata gtaattccta gtgtacgtaa atatgcaatt tttttgatgt cctctacatt
     5161 ttcatctgaa tattctctat agccattacg ctcattacga aggggtttaa ttaaattctc
     5221 gttctcataa aaacgaatat tagagcgtgt caatcctgtt ttttcttcaa cttctttaat
     5281 tgtcatttta aacactacct ctctattcta ttaatttaca actataatat agtcttaaat
     5341 attttcactc tataaaattg tatatttagt atatcttaaa atcaagttaa ttatctttta
     5401 taaattttca tacaataata tctcctttca ataatatctc tttttctata tttaaaacta
     5461 tcttattttc tataaaataa tgtataaaca aggtttacag tcaagtattt ttattatttt
     5521 tttgatttca taaaaaaaag catagcaaat catttgctat acttttttac taaattattt
     5581 attctatatc ttatatgaac ttattgttta actactacac aatactagct ttaatcatca
     5641 tattttttgc aaacatactg gcttcatata tttcttgttc atctggatga ctttgagatt
     5701 cctcatggtt atttaattgt tggcgaatat gttcgttgtc aggagaaagt tttaacatct
     5761 ctttatattt tgctttcaat ccttctgcta tcttaccttg acatatgaat gaacctaata
     5821 tttcattatc ttttgggata attgattcta tcttttctat tatactagct ccataacttc
     5881 cttttttact tgctcccatt gttccaaata gtgctatctt tttattgtga atattttcaa
     5941 gcacttgttt tgaattttta tcacaaattc ccttgtccac ccaatagcct aagaatacta
     6001 tatcataatt ttcatagtca ataccat
//
LOCUS       DAMJWP010000208         5944 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.215, whole genome shotgun sequence.
ACCESSION   DAMJWP010000208 DAMJWP010000000
VERSION     DAMJWP010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5944
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.215"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            66..1418
                     /locus_tag="PC700_003085"
     CDS             66..1418
                     /locus_tag="PC700_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2582359.1"
                     /translation="MTVTITGWIILSFLFLCIIIYILYSKAKSQKTIEKVAYTDFVTG
                     YSNWRKFELDVTNLLKKTSQNNKYAMVIFDIDKFKAINDIYGHKKGNLILKDIADTLN
                     ELTNINETFARVSADNFNILLTYNKKEDIINIIKKIIANNELVNLSFGIYEIKDKDLS
                     VSVYSDRASLAKSSIKNNSDVNFAFFNDKLREKLLLEDKIEKEMEYALESGQFVMYLQ
                     PKYNIKLDKFCGSEALVRWQYTEKEVIYPGDFIPIFEKNGFIRKIDMYILEQACKEIR
                     SLFDKGISPLPISVNFSRVDFLKKDFIENIVNICDRYKIPYSLIEIEITESSMFGDTD
                     TLFNVSRNLQDIGFIVAMDDFGSGYSSVNMLKNIPLNVIKLDRGFFVDDKDVDKSQIV
                     IKSIVSLIKQLGIRVVAEGIETRSQIEMLKKANCDIVQGYYFSKPLPIKEFEKLVYKI
                     "
     gene            1451..4888
                     /locus_tag="PC700_003086"
     CDS             1451..4888
                     /locus_tag="PC700_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDF2582360.1"
                     /translation="MIRAKSLKNVLSIVLIFLFTIISFSDFAFKTNVQEEENSKINLR
                     EISLQSSNAIKIKLDSSLSFIKSVANMVSYFNTIDNSKIVPLLANISKESDFQRISIV
                     SPDGISHTSDGKIINVSKYQYFKKAIKGESNISEVVKSPIDGKDTFVIATPIFNKNNK
                     IIGVLYGSYYNDKLAEFIDITSFNSEGYIDIFESSGQFVLKSSHTNKLIKSSSNIYDI
                     FKKASFDDGYSYKKIYEDTKNNKSGFTSYEYKGENRYATYTPIGVNDWYVISVIPQYT
                     VRRNIDKINQLAFSLIFKVFLLFIMLIIYIIYKNNKSQKDILKANKEISSITNSIPGG
                     VQKCTLGETCNINFLSDGFIELFGYTREEIKTLFNNDFYSLIHKDDVERIKKEISKQL
                     YKNKTIELEYRIITKNGNTVWVLDKGELITEPDGKEYFCCVTVDITRTKQLEQEMEQK
                     NLELEMISENILGGIIITEFNDDFTIKYTNSGYLSMIGYTREQLENELKYKACNLVFY
                     EDRKKALESIQHQLNTKGIAVYQHRIRRRDGNIIWVSIKGRLIIDDDGIEKGIWILND
                     ITDLKNAEQKLKINEERFRIALKQTSNTIFEYDIKTKSLLNTSKELDKYNIYKITKNV
                     PYSLVESGVIHKDFSDDFINMYENVINGAKKASCVVKMKLPDENYIWNKVTLTTIFDE
                     DGNPVRAIGIQEDISKQKEAEIRYNQEGKYHSAILSEAMITYEINVSKDTFIKGNESW
                     QKPFGIKPDNNYSEMVKTISNSIIHKDNKEVFLNTFLCKNLLSAYNKNITEIKLEYRR
                     MNQENKMIWVICTMHLIKDSITNDIKALAYVKDIDKQKNEEIKLKLKAERDTLTGLYN
                     KGTTENLINDFLNSDEASLGMHAFLIVDIDNFKSVNDNLGHVFGDKVLFEVSKHLKPI
                     FRKDDIVGRIGGDEFIVFLKNIQSFENTKKKAEKICDIFRNSYTENKKTYKISGTVGI
                     SFVPEHGTVFEELYKKADIALYYAKSKGKDKYALYNNCIYQNELENNISDNNIKLANK
                     CEKSKPVLKEILDSIDNYIYIVNPNTYELLFVNNKVSRLDLEIKLGETCYKSLLKSER
                     PCNNCPLNGLEENKHSKFKIETNTFGKNISIASWINCIDGEYNCLIECIDSSLYKNK"
     gene            4906..5652
                     /locus_tag="PC700_003087"
     CDS             4906..5652
                     /locus_tag="PC700_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDF2582361.1"
                     /translation="MLNSITLILGIIFTIYYFYLKIVFGGISFSEIFLVVGIVLIIYQ
                     IFKKKIKEKKVFYRVLKIIISIFLIVFVVTESLIIFYPKNSLESKSDYLLILGASVKK
                     TTPSTTLKGRLDTALKYLKINDNCYVVVSGGKGSGEKITEAKAMKDYLIKNSIDKNRI
                     IEEDKSTNTYENFKYSKLKIEEHSQRKLSDLKVKIVTTDFHVLRSKILAYRNGYKNTS
                     FYASKSKLSFVPTYYTREFFALWKTIIFDR"
     gene            complement(5601..5921)
                     /locus_tag="PC700_003088"
     CDS             complement(5601..5921)
                     /locus_tag="PC700_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDF2582362.1"
                     /translation="MEKIMNFLKENSTFYFATVEDNKPRVRSFGFIMDYEGKLYFGIV
                     KYKTSYKQLLVNPNVEISTTSKVGKWIRIKGIEKTRYEFISSLFIYYLSKMIVFHNAK
                     NSLV"
BASE COUNT         2484 a          595 c          891 g         1974 t
ORIGIN      
        1 gtgtatttac attcttttat ataggtaata ttagtagagt attagaatat gaaacaaatg
       61 acataatgac agttacaatt actggatgga ttatactttc atttttattt ttatgtatta
      121 taatttacat attatattca aaagcaaaga gtcaaaaaac tatagaaaaa gtagcatata
      181 cggattttgt aacaggttat tccaactgga gaaagtttga attagatgtt acaaatttat
      241 taaagaaaac atcacaaaat aataaatatg caatggtcat attcgatatt gataaattta
      301 aagcaattaa tgatatatat ggacataaaa agggcaattt aatattaaaa gatattgcag
      361 atactttaaa tgaattgact aatatcaatg aaacttttgc tcgtgtaagt gctgataatt
      421 ttaatatatt attaacatat aacaaaaaag aggatataat aaatattatt aaaaaaatta
      481 tagcaaataa tgaacttgta aacttatctt ttggaattta tgaaatcaaa gataaggatt
      541 tatctgtaag tgtatatagt gatagagctt cacttgcaaa atcatcaata aaaaataata
      601 gtgatgtaaa ttttgcattt tttaatgata aattaagaga gaaattatta cttgaagata
      661 agatagaaaa agaaatggaa tatgctcttg aaagtgggca attcgtaatg tatttacaac
      721 caaaatataa tattaaatta gataaatttt gtggaagtga agccctagtc agatggcaat
      781 atacagaaaa agaagtcata tatccaggtg attttattcc tatatttgaa aaaaatggat
      841 ttattagaaa aattgatatg tacatattag aacaagcttg taaagaaatc cggtctttat
      901 ttgataaagg aatttcacct ttacctatat ctgtaaactt ctcaagagtt gattttttaa
      961 aaaaagattt tattgaaaac atagtaaata tatgtgatag atataaaata ccatatagtt
     1021 taatagaaat tgaaattact gaatctagta tgtttggaga tactgatact ctatttaatg
     1081 ttagtagaaa cctacaggat attggtttta tagttgcaat ggatgatttt ggctcgggat
     1141 attcttctgt aaatatgctt aaaaatatac cattaaatgt aataaaatta gatagaggat
     1201 tttttgttga tgataaagat gttgataaga gtcaaattgt tataaaaagc atagtttctc
     1261 tcattaagca attaggaata agggttgttg ctgaaggaat tgaaacaaga agtcaaatag
     1321 aaatgttaaa aaaagcaaat tgtgatattg ttcaaggata ttatttttca aaaccattac
     1381 caattaaaga atttgaaaaa ttagtctata aaatatagtc agcgagtctt aaatgggagg
     1441 tggtgattaa gtgataagag caaaatcgtt aaaaaatgta ctgagtattg ttttaatatt
     1501 tttatttaca ataatttctt tttctgattt tgcatttaaa actaatgtcc aggaagaaga
     1561 aaatagtaaa attaatttaa gggaaatatc cctacaaagt tccaatgcta taaaaataaa
     1621 attagatagt tctctatcct ttattaaaag tgtggcaaat atggtttctt attttaatac
     1681 tatagataac tcaaaaatag ttccattatt agctaacata tcaaaagaat ctgactttca
     1741 aagaatctct attgtatctc ctgatggaat aagtcatacc agcgatggca aaattatcaa
     1801 tgtgtcaaaa tatcagtatt ttaagaaagc tattaaagga gaaagcaata tatctgaagt
     1861 tgtgaaatct ccaattgatg gaaaagatac ttttgttata gctactccta tatttaataa
     1921 gaataacaaa ataattggtg ttttatacgg ttcttactac aatgataaat tagctgaatt
     1981 tatagatata acaagtttta atagtgaagg gtacatagat atatttgaat ctagtggtca
     2041 atttgtttta aaatcttcac atacaaacaa actgattaag tcaagtagta acatatatga
     2101 tatatttaaa aaagcttctt ttgatgatgg ttatagctat aaaaaaatat atgaagatac
     2161 aaaaaataac aagagtggct ttacctcata tgaatacaaa ggagaaaatc gttatgctac
     2221 ttatacaccc attggtgtaa atgactggta tgtaatttct gtgataccac aatatactgt
     2281 aagaagaaac attgataaaa taaatcaatt agcattttct cttattttca aagtattctt
     2341 attgtttatt atgcttataa tatatattat ttataaaaac aataaatctc agaaagatat
     2401 actaaaagct aacaaagaaa tctcatctat tacaaatagc attcctggag gagtacaaaa
     2461 gtgtaccctg ggtgaaacat gcaatattaa ttttttaagt gatggattta tagagttgtt
     2521 tggttataca agagaagaaa taaaaacatt gtttaataat gatttttata gtttaataca
     2581 taaagatgat gttgaaagaa taaaaaaaga aatttctaaa caactttaca aaaataaaac
     2641 tattgaatta gaatatagga ttataacaaa aaatggtaat acagtatggg ttttagataa
     2701 aggagaactt ataacagaac ctgatggaaa agaatatttt tgttgtgtaa ctgtagatat
     2761 tactagaaca aaacaattag aacaagagat ggaacaaaaa aatctagaac ttgaaatgat
     2821 atcagaaaat attttaggtg gtataattat aacagaattt aatgatgatt ttactataaa
     2881 atacactaac tctggttatc tttcaatgat tggatataca cgtgaacaat tagaaaatga
     2941 acttaaatac aaagcttgta atcttgtttt ttatgaagat agaaagaagg ctcttgaaag
     3001 tatacaacat caattaaaca caaaaggaat agctgtatat caacatagaa taagaagaag
     3061 agatggtaat attatctggg tttctataaa aggaaggctt attattgatg atgatggtat
     3121 tgaaaaaggt atttggattc taaatgacat aacagattta aaaaatgcag aacaaaaact
     3181 taaaataaat gaagaaagat ttagaattgc attaaagcaa acatcaaata ctatatttga
     3241 gtatgatata aaaacaaagt cacttttaaa tactagtaaa gagcttgata aatataatat
     3301 ttataaaatt actaaaaacg ttccatattc tctagtagaa tcaggagtta ttcataaaga
     3361 ttttagtgat gattttataa acatgtatga aaatgtaata aatggagcaa aaaaagcaag
     3421 ttgtgtagtt aagatgaaac ttccagatga aaattatata tggaataaag ttaccttgac
     3481 tactattttt gatgaagatg gtaatcctgt aagagcaatt ggtatacagg aagatataag
     3541 caaacaaaag gaagcagaga taagatataa tcaagaaggt aagtatcatt cagctatatt
     3601 gtcggaagca atgataacct atgaaataaa tgtatcaaaa gatactttta tcaaaggaaa
     3661 tgaaagttgg caaaaaccat ttggaattaa gcctgacaat aattattctg aaatggtaaa
     3721 aactatatca aacagtatta ttcacaaaga caataaagag gtgtttttaa atacattttt
     3781 gtgtaaaaat ttattatctg catataacaa aaatataact gaaataaaat tagaatatcg
     3841 aagaatgaat caagaaaata aaatgatttg ggtcatttgc acaatgcact taataaaaga
     3901 ttctattacg aatgatataa aagcacttgc ttatgttaaa gatattgaca agcaaaaaaa
     3961 tgaagaaatt aaattaaaat taaaagctga aagagatact cttacaggat tatataataa
     4021 aggtactaca gaaaatttga ttaatgattt cttgaactct gacgaagcaa gcttaggaat
     4081 gcatgcattt ttaattgttg atatagataa ttttaaatct gtaaatgata atttaggaca
     4141 tgtatttgga gataaagtat tatttgaagt ttctaaacac ctaaaaccta tatttagaaa
     4201 agatgatata gtaggtcgaa ttggtggaga tgaatttata gtattcttaa aaaatattca
     4261 gtcttttgag aataccaaaa agaaagcaga aaaaatatgc gatatattta gaaattctta
     4321 tacggaaaat aaaaagacat ataaaatatc tggaacagtt ggaatttcat ttgtaccaga
     4381 acatggaact gtatttgaag aactttataa aaaagcagac atagcacttt attatgcaaa
     4441 gagcaaagga aaagataagt atgcacttta taataattgt atctaccaaa atgaacttga
     4501 aaataatata agtgataata atataaaatt agcaaataaa tgtgagaaat ccaaaccagt
     4561 acttaaagaa attcttgatt ctattgataa ttatatttat atagtgaatc caaatactta
     4621 tgaacttctt tttgtcaata ataaggtgtc tcgtttggat ttagagataa aattaggaga
     4681 aacctgttat aaatcactat taaaatctga gagaccatgt aataattgcc ctcttaacgg
     4741 attagaagaa aataagcatt caaaattcaa aatagagaca aatacatttg gtaaaaatat
     4801 cagtatagca agttggatta attgcataga tggagaatac aactgtctta tagagtgtat
     4861 agattcttct ttatacaaaa ataaataaaa aaggactatg aagatatgtt aaatagtata
     4921 actttgatac ttggtataat atttacaatt tactattttt atctcaaaat agtttttgga
     4981 ggaatttcat ttagtgagat tttcttagtt gtaggaattg tactaattat ctatcaaata
     5041 tttaagaaaa aaattaaaga aaagaaggtt ttttataggg tgttaaaaat aataatcagc
     5101 atttttttaa tagtatttgt agttactgag agtttaatta ttttttatcc taaaaatagt
     5161 ttagaatcaa aaagtgatta tctcttaatt ttaggagctt ctgtaaaaaa gactacacca
     5221 agtacaaccc tgaagggtag gttagacact gcattgaaat acctaaaaat taatgataat
     5281 tgttatgtgg tagtatctgg tggtaaagga agtggagaaa aaataactga ggcaaaagct
     5341 atgaaagatt atcttataaa aaatagtata gataaaaata ggataataga agaggataaa
     5401 tccacgaata cttatgagaa ttttaaatat tcaaaattga agatagaaga acatagtcaa
     5461 agaaaactaa gtgatttaaa agtaaaaata gtaacaacag attttcatgt tctcagaagt
     5521 aaaatattag catatagaaa tggatataaa aatacgtctt tttatgctag taaatcaaaa
     5581 ctttcatttg taccaactta ctatacaaga gaattttttg cattatggaa aacaatcatt
     5641 tttgatagat aataaataaa aagactagat ataaactcat atctagtctt ttctattccc
     5701 ttaattctaa tccattttcc aactttactt gttgtagaaa tttcaacatt gggatttaca
     5761 agtaattgct tataagaagt tttatatttt acgattccaa aatataattt tccttcataa
     5821 tccattataa atccgaatga tcttactctt ggtttattat cttcaactgt agcaaaatag
     5881 aatgttgagt tttcttttaa aaaattcatt attttttcca tgattaaatc ctctttgtat
     5941 ctta
//
LOCUS       DAMJWP010000209         5888 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.216, whole genome shotgun sequence.
ACCESSION   DAMJWP010000209 DAMJWP010000000
VERSION     DAMJWP010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5888
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.216"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            129..1493
                     /locus_tag="PC700_003089"
     CDS             129..1493
                     /locus_tag="PC700_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HDF2582363.1"
                     /translation="MDIKELVKMQRKYYNTGKTRDISFRIEQLKKLKLVVSQNEEKIL
                     LALKKDLNKSNFEGFMTEVGMFYSELNFAIKNIRKWSKIKRVKSSMVNFPSISKVVPQ
                     PYGVTLIMSPWNYPFQLALIPLVWSLAAGNCVILKPSEYSVSTSSVVKDIVENTFSKE
                     YVAVVQGSQEESEKLLLERFDYIFFTGSTNVGKIIMKSASEHLTPITLELGGKSPCII
                     LKDANIDLTAKRLTWGKLINAGQTCVAPDYVLVHEDRKNELIEKIKYYTNKYFGDNPC
                     NNEQFPKIINQRHFNRILSLIDKDKIVYGGNYNKETLKIEPTIVDNVNWDDNIMKEEI
                     FGPIFPILTYKDLDEVIQKIIQMPNPLALYIFTKNKYLENKLLEMIPAGGCCINDTVT
                     HIATNYLPFGGIGESGMGSYHGKAGFDTFTHYKSVLKKLNLDVPIRYAPYDNKLIKVL
                     KKIM"
     gene            1632..2798
                     /locus_tag="PC700_003090"
     CDS             1632..2798
                     /locus_tag="PC700_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDF2582364.1"
                     /translation="MKINWNTKYTTIAIYTFIIAASSIIFYLVSSQIDVFSNNLDAIF
                     TTLQPFIIGFAIAYLLNFILKFYEDRIFIKSEKLKKLKQSSKRGLGLLLTYATATLIL
                     YLFMHFVLPQVIESIVGLANDIPMYVNNATKLIDKLMTDLNLDEQYFNLAVDKWNEFV
                     TYIIKFVTDLIPILGSMLKNVASSIWNIVLGLIVSVYLLIDKEKFYGLSKKITYAVFT
                     EKQAARILELTHRSNYTFGRFLGGKILDSFIIGILTFVILTLVKMPYTLLISVIIGIT
                     NIIPFFGPLFGAIPSTLIVMFVSPIKAFWLLLIILIIQQIDGNIIGPKILGDSIGISA
                     FWILFSLLVAGKLLGFIGMVIGVPMFAVIYSIIKDIVESKLDKKGLPTDTSDYM"
     gene            2915..3697
                     /locus_tag="PC700_003091"
     CDS             2915..3697
                     /locus_tag="PC700_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDF2582365.1"
                     /translation="MAFIECKFKSKYTGGETDIIVILPVANGKELFSGKEIYDFEGKF
                     KTLYLFHGLEDDQSSWIRYTNVEHYAKNKNIALVIPRVETSFYTDMAYGHQYFTFVSE
                     ELPKFVRAIFPLSDKREDNFVAGMSMGGYGALKLALSKPKEFSAVASFSGSPDMIHEL
                     ENQQIETNADILFHDFGRPEDARNSENDLLYILKNLKERNEDIPKIYQFCGDRDFLYK
                     NNQMFKKYAESLGVDVEYEECVGGHEWRLWNLWIKKFIDIIG"
     gene            complement(3816..4469)
                     /locus_tag="PC700_003092"
     CDS             complement(3816..4469)
                     /locus_tag="PC700_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2582366.1"
                     /translation="MSKSEKGKETKASIISAARELFFEQGYHKTTTRQIAERANINLG
                     LISYYFSSKSEIGAIIYEDIRNEMQSLIYNYHEEGTMMMFFLAMNADLCFLLKNEAYR
                     NFYLDIAYEEAILSYQRSVTEMVMKRYVNDKESIEHDELILSCIGILAMKPEIIRVYT
                     SHKYDISSQAVIKHFTKQFLINLGHSTDLCEEILEELSKYYIDIVDNFTPIITRVRT"
     gene            complement(4493..5857)
                     /locus_tag="PC700_003093"
     CDS             complement(4493..5857)
                     /locus_tag="PC700_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HDF2582367.1"
                     /translation="MLLLIVTCAIYIWIGFYFSKKVKTASDFLIAGRNLPLPILAATI
                     AATSFGGGCLVGGVQWGAERGLWVGMYSTIGAALACFINAFFAGKLRSLSCDITPADY
                     IETRYGHSVFLRAYQSLVTPISILAIMGSQLISFGSVSTAFGIPYDIAVIIGVIVIII
                     YTYTSGMWGVAVSAFIQLAICIVFLPIVAYISLKLVGDNPLLMLQSMVKEPFFPGNKS
                     ISDFMYTVLPLVLGSIFSYESFLRYQCADNAKNAVKSSVIAGFILLFLAFPIGIIGAI
                     GGSLFPNISSVQVLPQMISTTLPPLISVLFLAAILAAIMSTADSLMTSMSAIISRDIY
                     NKLLHPNIEFNDLKNTLKYSQIASALTAIIGAIIALKFDSLLELLFWPAPLCTGVIFA
                     PFVIGLCWKGATKKGAIYAVIVSVILALLNMIGILTVFDRILVPIIGGSITIFIVSKF
                     SKKD"
BASE COUNT         2210 a          748 c          900 g         2030 t
ORIGIN      
        1 tttttttatt ggaagattta tgttatattg agatgaattt attatgatat aaaaaataat
       61 gttaggataa atttaaatct ttttctaaaa atgtattaat ataattctta aagaaaggat
      121 ttttaacaat ggatataaaa gagttagtta aaatgcaaag aaaatattat aatacaggta
      181 aaacaagaga tatatcgttt cgtatagaac agttgaagaa acttaagtta gttgtatcac
      241 aaaatgaaga aaaaatactt ttagcactaa agaaagattt aaataaatcg aattttgaag
      301 gttttatgac agaggtaggc atgttttata gtgaattaaa ctttgctata aagaatataa
      361 gaaaatggag caaaataaaa agagttaaat caagtatggt taattttcca tctatatcca
      421 aagttgttcc acaaccttat ggtgttactc tcataatgtc accttggaat tatccctttc
      481 aattagcact tataccatta gtttggtcat tagcagcagg gaattgtgtt atattaaaac
      541 cttctgaata ttctgtgtca acatctagtg tagttaaaga catagttgaa aatacttttt
      601 caaaagaata tgttgcagtt gttcaaggaa gtcaagaaga aagtgaaaag ttactattgg
      661 aaagatttga ttatatattt tttacaggaa gtactaatgt tggtaaaata atcatgaaat
      721 cagcatcaga gcatttaact cctattactt tagaacttgg aggaaaaagt ccttgtatca
      781 ttttaaaaga tgctaatata gaccttactg ccaaaagact tacttgggga aaactaataa
      841 atgcaggaca aacctgcgtg gctccagatt atgttttagt gcatgaagat agaaaaaatg
      901 agttaataga gaaaataaaa tattatacta ataagtattt tggagataat ccatgtaata
      961 atgagcaatt tcctaaaatc ataaatcaga gacattttaa tcggatatta tctcttattg
     1021 ataaagataa gatagtctat ggtggaaatt ataataagga gacattaaag atagagccaa
     1081 ctatagtaga caatgttaat tgggatgata atataatgaa ggaagaaata tttgggccta
     1141 tatttcccat tttaacatat aaagatttag atgaagtaat acaaaaaatt atacaaatgc
     1201 caaatccatt ggcactttat atttttacaa aaaataagta cctagaaaat aaacttttag
     1261 aaatgattcc agcaggcgga tgttgtatca atgatacagt tactcatata gctacgaatt
     1321 acttaccttt tggtggtatt ggtgaaagtg gtatgggctc atatcatgga aaagctggat
     1381 tcgatacatt tactcactat aagagtgtgt taaaaaaatt aaatttggat gtccctatta
     1441 gatatgctcc atacgataat aaattaatta aggttcttaa aaagataatg taggttatat
     1501 tgtagaataa taaatataaa taaaattgag tatttgtatt ctgaatatat tttattttta
     1561 atttggtata tttgttataa aagtggtgat tttgtgataa aatttaatta tatagaaata
     1621 aggaggaaaa aatgaaaatt aattggaata caaaatacac aacaatagca atttatacat
     1681 ttataatagc tgctagtagt attatatttt atctagtatc atcacaaatt gatgtatttt
     1741 ctaataattt agatgctata tttacaacat tacaaccttt tataatagga tttgctattg
     1801 catatcttct taattttatt ttaaagtttt atgaagacag aatttttata aaaagtgaga
     1861 aattaaaaaa actaaaacaa agttcaaaaa gaggcttggg tcttttatta acatatgcaa
     1921 cagctacatt gatactttac ttatttatgc atttcgtatt gccacaagta attgagagta
     1981 ttgttggact agcaaatgat atacctatgt atgtaaacaa tgcaactaag cttattgata
     2041 aattaatgac tgatttaaat cttgatgagc aatactttaa tttggcagtt gataagtgga
     2101 atgagtttgt tacatatatt ataaagtttg taacagattt aatcccaata ttaggaagta
     2161 tgttaaagaa tgtagcctct agtatatgga atattgtact aggattaatt gtttcagtat
     2221 acttattaat tgataaagaa aaattctatg gattaagcaa aaaaataact tatgcagtat
     2281 ttacagaaaa acaagctgcc agaattttag aattaactca tagaagtaac tatacatttg
     2341 gaagattttt aggtggcaaa atattagatt cattcattat aggtattcta acatttgtaa
     2401 tacttacatt agttaaaatg ccatatacat tattaatatc agttataatt ggtattacaa
     2461 atattatacc tttctttgga cctttatttg gagctatacc atctactcta atagtgatgt
     2521 ttgtatctcc tataaaagct ttctggttat tgttgattat attaataata caacagatag
     2581 atggaaatat tataggacct aaaatacttg gagattcaat tggtatatct gcattttgga
     2641 ttttgttctc cttattggtt gcaggaaaat tattaggttt tattggaatg gttataggag
     2701 ttccaatgtt tgcagtaatt tattctataa ttaaggatat tgttgaaagt aaattggaca
     2761 aaaagggatt accgacagat acttccgatt acatgtagct aaaaaatatt atgtataatt
     2821 aaaaatatat aaattaagca atatttaaag cactgagaaa gttaaactct tggtgctttt
     2881 tagatataaa attattatag aagaggtgtt ttttgtggca tttatagaat gtaaatttaa
     2941 atctaaatat actggaggag aaactgatat aattgtaatt ttgcctgtag caaatggaaa
     3001 agaacttttt agtggtaaag agatatatga ttttgaaggt aaatttaaaa cattatactt
     3061 atttcatggt ttagaagatg accaaagctc atggattaga tatactaatg tagagcatta
     3121 tgctaaaaat aaaaatatag ccctagtaat accaagagta gaaactagtt tttatacaga
     3181 tatggcgtat ggacaccaat attttacttt tgtaagcgag gaattaccta aatttgttag
     3241 agctatattt cctttatcag ataaaaggga agataacttt gtagctggta tgtctatggg
     3301 gggatatgga gctttaaaat tagcactttc aaaaccaaaa gagttttcgg cagttgcaag
     3361 tttttctggt tcgcctgata tgattcatga gttagaaaat caacaaatag aaaccaatgc
     3421 agacatttta tttcacgatt ttggtagacc agaagacgct agaaatagtg agaatgattt
     3481 actttacata cttaagaacc ttaaagaaag aaatgaagat attcctaaaa tttatcaatt
     3541 ttgtggtgat agagattttt tatataaaaa taatcagatg tttaaaaaat atgcagagtc
     3601 tctgggtgta gatgttgaat atgaagagtg tgtagggggg catgaatggc gtctttggaa
     3661 tttatggata aagaaattta tagatataat tgggtagtac acttatggta ttaacaggta
     3721 tactataaaa tatctttaaa tatgataaag actgaaatcc taaaatcaaa ttatgatttt
     3781 aaaatttcag tctataagaa tttaataaaa ttttattaag ttcttacacg agttattatt
     3841 ggagtaaaat tgtcaactat atctatatag tatttactca attcctctaa aatctcttcg
     3901 cataaatccg tactatgacc aagattaata agaaactgtt ttgtaaaatg ctttattaca
     3961 gcttgagaag atatatcgta cttgtgtgaa gtataaactc taattatttc aggtttcata
     4021 gcaagtatac caatacaaga aagtattaat tcatcatgtt ctatagattc tttatcatta
     4081 acatatcttt tcatgaccat ttcggttaca cttctttgat agcttaaaat agcttcttca
     4141 taagctatat ccaaataaaa atttctatat gcttcgtttt ttagaaggaa acaaaggtca
     4201 gcattcatag caagaaagaa catcatcata gtaccttctt catggtagtt atagattaaa
     4261 gactgcattt catttcttat atcttcatat attatagctc ctatttcgct tttacttgag
     4321 aagtaatagc ttattagacc taaatttata ttagctcttt ctgcaatttg cctagttgtt
     4381 gtcttatgat aaccttgctc aaaaaataat tctctagcag cacttataat actagctttt
     4441 gtttctttgc ccttttctga ttttgacata tttactgtgt atacctctct tttcaatctt
     4501 tttttgaaaa tttgctaact ataaatattg taatagaacc acctattata ggaaccaaaa
     4561 ttctgtcaaa tacagtaaga ataccaatca tatttaataa tgccaatata acacttacta
     4621 taacagcata gatagctcct ttttttgtag cgcctttcca gcaaagacca attacaaatg
     4681 gtgcaaatat tacacctgta cataatggag ccggccaaaa caataattct aataaactat
     4741 caaatttaag tgctatgatt gcacctatta tagctgtcaa agctgatgca atttgagaat
     4801 actttagagt attttttaaa tcattaaatt ctatattagg atgtaataat ttgttataaa
     4861 tatctcttga tataatagca ctcattgagg tcattaatga atcggcagta gacattatgg
     4921 cagccaatat agcagctaag aataatacag atattaaagg tggcaaagta gtcgatatca
     4981 tttgaggtaa aacctgaaca gatgatatgt ttggaaataa actaccacca atagctccaa
     5041 ttattcctat agggaaggca agaaataata gtataaatcc tgcaattaca ctacttttaa
     5101 ctgcattttt agcattatca gcacattgat atcttaaaaa actttcataa gaaaatattg
     5161 agcctaaaac taatggtaaa actgtataca taaaatcact aatcgattta tttcctggaa
     5221 agaatggttc ttttaccatt gattgtaaca taagtaaagg gttgtcgcca actaatttta
     5281 agcttatata agctactatt ggcaaaaaca ctatacaaat agcaagttga ataaatgctg
     5341 atactgcaac tccccacatt cctgatgtat aagtatatat aattataact ataactccta
     5401 taattacagc tatatcataa ggaataccaa atgcagtaga tacagaacca aaagaaatta
     5461 attgactacc cataatagct agtatagata ttggtgttac aagactttga taagctctta
     5521 aaaatacact atgaccatat ctagtttcaa tataatcagc aggtgttata tcacaagata
     5581 agcttcttaa ttttcctgcg aaaaaggcat ttataaagca agctaaagct gcacctatag
     5641 tagaatacat tccaacccaa agtcctcttt cagctcccca ttgaactcca ccaactaagc
     5701 accctcctcc aaaagatgta gcagcaatgg ttgcagctag tataggcaaa ggaagatttc
     5761 tacctgcgat taggaaatcg gatgctgttt taactttttt tgaaaaataa aaaccaatcc
     5821 atatgtatat tgcacacgta acaattaaaa gtaacatagt cttttctcct ttataaaata
     5881 ttttaata
//
LOCUS       DAMJWP010000210         5879 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.217, whole genome shotgun sequence.
ACCESSION   DAMJWP010000210 DAMJWP010000000
VERSION     DAMJWP010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5879
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.217"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            88..654
                     /locus_tag="PC700_003094"
     CDS             88..654
                     /locus_tag="PC700_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2582368.1"
                     /translation="MSVSLQKGQRVSLVKDNNPVKNLVVGLGWDMNKLGKKNYDLDAF
                     AIALTNQDKMNVRGDLVYFGNLKHPSKAIIHTGDNLTGKGEGDDEQIIVNLEDIPEYV
                     HKIVFGVSIYKAKKRDQDFGQMNNSFIRLIDSNSKQELFKYNLQEDFSGKVTVLAGEI
                     YRRNEEWKFNALGIGQNEELRELINTYK"
     gene            694..1275
                     /locus_tag="PC700_003095"
     CDS             694..1275
                     /locus_tag="PC700_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2582369.1"
                     /translation="MGITLAKGQKVSLTKSNPGLKKVIVGLGWDINKYDGGFDFDLDA
                     SAFLTGTDGKVTNDGDFIFYNNLKHASGAVEYMGDNRTGVGDGDDEQINVDLSKIPQN
                     IAKISFSVTINEAITRRQNFGQVENSYIRIYNEETNEELIKYELGEDFSIETAIVVAE
                     LYRHNGEWKFNALGSGFEDGLAGLCKNFGVNIG"
     gene            complement(1332..2249)
                     /locus_tag="PC700_003096"
     CDS             complement(1332..2249)
                     /locus_tag="PC700_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2582370.1"
                     /translation="MTIRSLEIFVKVAECGKMSEVARNMYITQSSVSQAISEIEKEYG
                     VKLFDRISKKLYLTEAGKKLLGYGRHLLAVNEEMNDCMKHCAKNIRIRVGATVTVGTC
                     VISPIMLELYKVNPLIEPEVFVEDTRLIAKKLLNSELDIAIVEGKIKHPDIVTKSIIN
                     DNLVLICSHKHEFYKRDSIKVSELSNQPLIMRELGSGTRAQLEKQLKELKIPMNIRWS
                     CYNSEAILRAVVDNFGIAVISELLIEDYLKKHLLWACDIEGINLHRTFDIAYHRSKFF
                     TENISAFFDISVEYGKKQARKKSRSVNSL"
     gene            2379..4010
                     /locus_tag="PC700_003097"
     CDS             2379..4010
                     /locus_tag="PC700_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF2582371.1"
                     /translation="MKVLIIGGVAAGTKTAAKIKREMGDNCSVTILNKGDDISYAGCG
                     LPYYVGKIIEDKSSLIVNTPESFSKLTGAEVRCGVEVTSIDRNAKQVTLKTLSTGEKE
                     NLSYDKLVIATGADPIKPPIPGIDLPGVFFMRTPNDAIKLRDSIENGIKRAVVIGGGF
                     IGLEIAENLSAMGIRVSVVDMAEHVMPGFDTDFAEYVENHMADKGIMIFTGDQVVGIE
                     GENKVEKLRTKNRAIKTDLVVMSVGIRPNTGFLKDTGLEFAPNNTIIADEYMLTNDKD
                     IYVVGDCAFVKNSLTNKPTWSPMGSSANHEGRICAQNISGKSKTYNGVLGTTVVKLPE
                     LNAGKTGLSKEIAEKEGYNVCSVTIATDDKAHYYPGASNFIIRMVAEKNTRKLLGVQV
                     MGPGAVDKIVDIAATAITLGADLYSLESMDLAYAPPFSTAIHPFVVAVNVLQNKLNGE
                     LDSVLLDEIEDFDSWTKLDVSKAPSIPSLRYIPVGEINGEIEGLAKDEKILLICAKGK
                     NSYMAQNRLRRFGYTNTKVLEGGILFYPNLKVMEE"
     gene            4013..4960
                     /locus_tag="PC700_003098"
     CDS             4013..4960
                     /locus_tag="PC700_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HDF2582372.1"
                     /translation="MSNVTAEQKKEVKGRGFLPSRDGEHFAARIITVNGVINASQAKK
                     IAEAAEKFGNGQLAFTTRLTVELPGVKFEDIEALSEFIASENLITGGTGSRVRPVVAC
                     KGTVCVHGLADTQALATEIHEEFYKNWYDVKLPHKFKIGVGGCPNNCIKPDLNDLGIV
                     GQRVPDYDSELCVGCKKCAVVEVCPVKAAKLTDKGKLEIDSNLCNNCGKCIESCNFDS
                     IEEKESGYKVYIGGKWGKSVRPGTQIDRLFSKEELMTLIEKAILLFREQGNTGERFGI
                     TIDRIGADKFIEMLLSDEVLERKEEILEALLHLTGGAVC"
     gene            complement(5245..5508)
                     /locus_tag="PC700_003099"
     CDS             complement(5245..5508)
                     /locus_tag="PC700_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1883 domain-containing protein"
                     /protein_id="HDF2582373.1"
                     /translation="MEYSYSKMNLKKGDIVEVNLEKQANVILLDHINYVKFKNQRNYD
                     YYGGFAKKNPCRMRVPNTGTWYLVVNQDGNSGIVNFSINTIQN"
     gene            complement(5607..5852)
                     /locus_tag="PC700_003100"
     CDS             complement(5607..5852)
                     /locus_tag="PC700_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582374.1"
                     /translation="MFIDEELEGYILTCKISEDFKNIPEYSDEEFYVTVYKDESSDSG
                     YYALLENKEERVVWDGEVVANNIFNNLWIVVNKVKTG"
BASE COUNT         2110 a          807 c          983 g         1979 t
ORIGIN      
        1 agcctaaata cgtatataat atagctaata tggttcttat acttgatacc atcaataaaa
       61 ctaaaaatat aaaaaggaga acttaaaatg agtgttagtt tacaaaaagg acaaagagtt
      121 agcttagtaa aagataataa tcctgtaaaa aatctagtag taggcttagg atgggatatg
      181 aataaattgg gtaaaaaaaa ttatgaccta gatgcatttg caatagcatt gactaaccaa
      241 gataaaatga atgttagagg tgacttggtt tattttggaa atcttaaaca cccttctaaa
      301 gctattatac acactggaga taacttaact ggaaaaggtg aaggtgatga tgaacaaata
      361 attgttaatt tagaagacat acctgaatat gtacataaaa tagtttttgg agtttctata
      421 tacaaagcta aaaaaagaga ccaagatttt ggtcaaatga acaactcttt tataagatta
      481 attgattcaa attccaagca agaattattt aaatataatc ttcaagaaga cttttctggt
      541 aaagtaacag ttttggcagg agaaatttat cgtagaaatg aagaatggaa atttaatgct
      601 ttaggaattg gtcaaaatga agagcttcgt gaattaatta atacatataa gtaataatta
      661 caataaaaaa tttataatga ggagttggtt taaatgggta taactttagc aaaaggacaa
      721 aaagtaagtt taacaaaaag taaccctggt ctaaaaaaag taatagtagg actaggatgg
      781 gatattaata aatatgatgg tggatttgat ttcgatttag atgcctctgc atttttaact
      841 ggaactgatg gaaaagtaac gaatgatgga gactttatat tctataataa tttaaaacac
      901 gcttctggag ctgttgaata tatgggtgat aatagaacag gtgttggtga tggtgatgat
      961 gaacaaataa atgtagactt atctaaaata cctcaaaata ttgctaaaat atcatttagt
     1021 gttactataa atgaagcaat tacaagaaga caaaatttcg gacaagttga aaattcatat
     1081 ataagaatat ataatgaaga aactaacgag gaacttatta aatatgaatt gggtgaagat
     1141 tttagcatag aaacagcaat agtcgtggca gaactatata gacataatgg agagtggaaa
     1201 tttaatgctt taggttctgg atttgaggat ggacttgcag gattgtgtaa aaattttggc
     1261 gttaatatag gctaatatat ctaaataaaa ataagagtat attgatatac tcttattttt
     1321 atttagatat attataaact gtttacactt ctactcttct ttctcgcttg ttttttccca
     1381 tattctactg aaatatcaaa aaatgctgaa atattttctg taaaaaactt gcttctatga
     1441 tatgcaatat cgaatgttct atgtaagtta ataccttcaa tatcacaagc ccacaaaaga
     1501 tgctttttta aataatcctc aataagtaat tcggatataa ctgcaatgcc aaaattatct
     1561 acaactgctc ttaatatagc ttcagagtta taacatgacc atctaatatt cattgggatt
     1621 tttaattctt ttaattgctt ttctaattgt gctcttgtac cacttcctaa ttcacgcata
     1681 attaatggtt gatttgaaag ttctgaaact tttatagagt ctcttttgta gaactcatgc
     1741 ttgtgtgaac atataagtac aagattatca ttaataatgc tttttgtaac aatatctggg
     1801 tgcttaattt ttccttctac aatcgcaata tctaattcac tattaagtaa tttttttgca
     1861 atcagtcttg tatcttctac aaatacttca ggttctataa gaggatttac cttgtataat
     1921 tctaacatta tcgggcttat tacacatgta ccaacagtta ctgttgcccc aactcttata
     1981 cggatatttt ttgcacaatg cttcatacaa tcattcattt cttcatttac agctaaaaga
     2041 tgcctcccat atccaagaag ttttttccct gcctcagtta aatataattt ttttgaaatt
     2101 ctatcaaata acttaacacc atactctttt tctatttctg aaatagcttg gctaactgat
     2161 gactgggtaa tatacatatt tcttgctact tcactcattt taccacattc agcaacctta
     2221 acaaagattt caagactacg aattgtcata ttatcacaac cttagcaaac attagtaaat
     2281 gcttatgatt ttattaatat tttagtattt tacaaatata ttgtcaagaa gtataatttt
     2341 attatagagt tataaagtag attggagtgt gatatattgt gaaagtatta attataggtg
     2401 gagttgctgc tggaacaaaa actgctgcaa aaataaaacg tgaaatgggt gataattgct
     2461 ctgttacaat actaaataaa ggtgatgata tttcttatgc aggttgtggt cttccatatt
     2521 acgttggtaa gataattgaa gataaatcat ctttaatcgt aaatacacct gaaagttttt
     2581 ctaaactaac aggagcagaa gttcgttgtg gagttgaagt tacgtctatt gatagaaatg
     2641 caaaacaagt aactttgaaa acgctttcaa caggagaaaa agaaaatcta agctatgaca
     2701 aacttgtaat tgctacaggg gcagacccaa taaaaccacc aatacctgga atagatttac
     2761 ctggagtatt ctttatgcgt actccaaatg atgctataaa actaagagat tctattgaaa
     2821 acggtattaa gagagctgtt gttattggtg gaggtttcat tggtcttgaa attgctgaaa
     2881 atctttctgc aatgggtata cgtgtaagtg ttgttgatat ggctgaacat gttatgcctg
     2941 gatttgatac tgattttgct gaatacgttg aaaatcatat ggctgataaa ggcataatga
     3001 tttttacagg tgaccaggtt gtaggtattg aaggtgaaaa taaagttgaa aaacttcgta
     3061 ctaaaaatag agctattaaa acagatttag tggttatgtc agttggaatc cgtcctaaca
     3121 caggttttct aaaagacaca ggtttagaat ttgcccctaa taatacaatt attgcagatg
     3181 aatatatgct tacaaatgat aaagatatat acgtagttgg agactgtgct tttgtaaaaa
     3241 attctttaac aaataaacct acatggtcac ctatggggtc atctgcaaat catgagggta
     3301 gaatttgcgc acaaaatata tctggaaaat ctaaaactta caatggtgtt cttggaacga
     3361 cagtagtaaa attacctgag ctcaatgcag gaaaaacagg tctaagtaaa gaaatagctg
     3421 aaaaagaagg ttataatgtt tgctctgtaa ccatagctac cgatgataaa gctcattatt
     3481 atccaggtgc atctaatttt attataagaa tggttgctga aaaaaatact agaaaattat
     3541 taggtgttca agttatggga cctggagcgg ttgataaaat agtagatatc gctgctactg
     3601 caataacttt aggcgctgac ctttattcat tagaaagtat ggaccttgca tatgcaccac
     3661 ctttttcaac agctattcac ccttttgtag tggcagtaaa tgttttacaa aataaactta
     3721 acggagaact tgatagtgtt ttacttgacg aaattgagga ttttgacagt tggactaaac
     3781 ttgatgtttc aaaagctcct tctattccta gtttacgtta tattcctgtt ggtgaaatta
     3841 atggagaaat tgaaggtctt gctaaggatg aaaaaattct tcttatatgt gctaaaggta
     3901 aaaatagcta tatggcgcaa aatcgcttaa gacgctttgg ctatacaaat acgaaagttc
     3961 ttgaaggtgg aatactattt tatccaaatc ttaaggtaat ggaggagtaa atatgagtaa
     4021 tgttacagca gaacaaaaaa aggaagtaaa aggtagaggt tttctaccaa gcagggatgg
     4081 agaacatttt gcagcacgta taataactgt aaatggtgta ataaatgcat cacaagcaaa
     4141 aaaaattgca gaagctgctg aaaaattcgg aaacggtcag ttagcattta ctactagact
     4201 aacagtagaa ttacctggtg taaaatttga agatattgaa gctctaagtg aatttattgc
     4261 tagcgaaaat ctaataacag gtggaactgg atcaagggtt cgtcctgttg tggcttgtaa
     4321 aggtactgtt tgtgttcatg gtctagctga tacacaggca cttgctactg aaatacacga
     4381 agaattttac aaaaattggt atgatgtaaa actacctcat aagttcaaaa ttggtgttgg
     4441 tggatgccct aataactgta ttaaacctga cctaaacgat cttggaattg ttggtcaacg
     4501 tgtacctgac tatgattcag agctttgtgt tggttgtaaa aaatgtgctg ttgtagaggt
     4561 ctgtcctgtt aaagcagcta aattaacaga taaaggtaaa cttgaaatag acagtaatct
     4621 atgtaataac tgtggtaagt gtatagaaag ttgtaatttt gatagtattg aagaaaaaga
     4681 atctggttat aaagtctata taggagggaa atggggaaaa tcagttcgtc ctggaacaca
     4741 gattgataga ttattttcaa aagaggaact tatgacttta attgaaaaag caattctttt
     4801 atttagagaa cagggtaaca caggtgaacg ttttggaata actatcgata gaattggtgc
     4861 tgataaattt atagaaatgc ttttatcaga tgaagttttg gaaagaaaag aagagatact
     4921 agaggctctt ttacacctta caggtggagc tgtatgctaa caataaatat tttaattgta
     4981 cagataacct atggaaaaaa ttttagttaa gaataattaa aattctatat atgatatatg
     5041 ataaatgagg ctatctcaaa acacagatac gcctcttttt cattgttgaa atgataaagt
     5101 atatttgagt ttatacaaaa aagagtgtct cgtgaattat aaaattcatt ttgaggcacc
     5161 cacattattt tataataatt gcttattaat tttattttaa ctatataaaa agtacaaaaa
     5221 gtaagaatat ccaatattct aaaattaatt ttgaattgta ttgattgaaa agtttactat
     5281 tccactattt ccatcttgat ttactactag ataccatgtt ccagtattag gaactctcat
     5341 tctacatgga tttttctttg caaatccacc atagtaatca taatttcttt gatttttaaa
     5401 tttcacataa tttatatggt ctaacaatat tacattggct tgtttctcta aatttacctc
     5461 tacaatatcc ccttttttta agttcatctt actataacta tattccattg aattactcct
     5521 ccagtatcga ataatctttg ttatttactc atctcaaata tgtaaaaatt catttaaaat
     5581 attacaaata ctatataatt agactattat ccagttttaa ccttatttac tacaatccaa
     5641 aggttattaa aaatattatt ggcaacaact tctccatccc atacaactct ttcttcttta
     5701 ttttctaata aagcatagta cccagagtca gaactttcat ctttatagac tgtaacataa
     5761 aactcttcat cactatattc aggtatattt ttaaagtctt cagatatttt acatgttaat
     5821 atataccctt ctaattcttc atctataaac aaagcaattc ctccaatctc tatattttt
//
LOCUS       DAMJWP010000211         5865 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.218, whole genome shotgun sequence.
ACCESSION   DAMJWP010000211 DAMJWP010000000
VERSION     DAMJWP010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5865
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.218"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            82..702
                     /locus_tag="PC700_003101"
     CDS             82..702
                     /locus_tag="PC700_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDF2582375.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(868..1404)
                     /locus_tag="PC700_003102"
     CDS             complement(868..1404)
                     /locus_tag="PC700_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDF2582376.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(1517..2191)
                     /locus_tag="PC700_003103"
     CDS             complement(1517..2191)
                     /locus_tag="PC700_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDF2582377.1"
                     /translation="MSLFIISQCITSFAFTPENNKFKVKPLPYAYDALEPYIDKETMK
                     LHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVRNNAGGAYNHK
                     FFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWAWLVATKDGKL
                     SIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVVNWNGALENYK
                     NLKSQD"
     gene            2394..4268
                     /gene="asnB"
                     /locus_tag="PC700_003104"
     CDS             2394..4268
                     /gene="asnB"
                     /locus_tag="PC700_003104"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDF2582378.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLREAFREIVPEYVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            complement(4360..5796)
                     /locus_tag="PC700_003105"
     CDS             complement(4360..5796)
                     /locus_tag="PC700_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDF2582379.1"
                     /translation="MICTIKPLKISIKKSTEPLIEFISDTTYKNKSSSYIKEVHFTRY
                     IGVDDSIDKYIEKVKNIDTYFFNNPTIPYIRLNNLKINFDKEQTDKMLKIFNIYDSSK
                     FNSWDLYNIDFPCKIENDTLNWTKKIAFKNTLDLFSTSMPNCTSSIIKNFAVKLLCWM
                     DYFLPKLFYNKVKTTISPKIVYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDLYP
                     ECSKFSTLFELSRKTPNILEIPFERHITNINYSENTNKTSPTTNKPNSKNTLNNTNLT
                     SKKIAQDEEVELSYEDLAKLSASVVMIVVCNNENKPFKSGSGVVINNEGYILTNLHVV
                     NDGYSFLVRFENDDKVYTSYQIIKYHSDYDLAVIKVDRKCKPIPVKVSKKPVRGQKIV
                     AIGSPLGLFNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLISAG
                     YDDGQNLNLAVESNLVKIFANNFIEIVN"
BASE COUNT         2254 a          683 c          859 g         2069 t
ORIGIN      
        1 agattcattt tttgagattg gtatataata aacatataga gtatataata atagttaaag
       61 ttaaaaaagg ggaaaagcaa tatgcagtat atacaagaat tacttgattt gactcaaaat
      121 tattgggtgt tagccacaat aatcggctta ttaagtgcat ttatagaaag ctttatacca
      181 gccttaccac ttgttgctat agttacagca aatgctgcaa tacaaggatt atttattgga
      241 tgtttgttat catggattgg ctcaggactt gggacaactt cattattctt attaataagc
      301 agatttaatg atagtaagct ttttaataag ttgagaaata gtaaaactga aaaagcaata
      361 agttggatgg ataaacaggg atttaagtta ttatttatag cgtatgcctg tccatttatg
      421 cctggttgtt tagttactat cgcatcagct tttggtaaaa aagatttaaa agattttgtg
      481 ccagcaatgt tggcaggaaa atttgtaatg tttatagtta taagttacgt ggcaagtgat
      541 atagaaggtt ttataacaag tcctttaaag atagcatcat ttatattatt ggtgtttttg
      601 tcatggaaga ttggaaatag agtaaataaa aatcttgaaa atcataatta tgatttccat
      661 cataaaaaac atcacaatga taaagatgat aaaatgattt agcttgtacg aaataaaacg
      721 tatcttaaaa tgatttagta ctaaattatt ttaagatacg ttttattttt tgtattaagg
      781 tacaaaattt ttagttcttt tgtaaactaa aatatataaa acttatttat acttagttca
      841 aaatattgtt atttattttt tctacaacta taataaaata taaaattgat atccaatcaa
      901 aaaaataact atgaaattta atagcttaga tatgaatttt tctgtttttg agttagtttt
      961 tataggtagt aaagaaatgt ttatggtaat gggataaaac aattcaatac cttctttagt
     1021 aataaggtca agacacaagt gagatagata tcctattata aacccactgg acaaacatga
     1081 gaaaactata ttattgttag tgaagattgt cagagattta aaaaaactat aaataagtaa
     1141 taaggcaatt agactgtgag taaaccctct atgtctaaac ctacttccaa ataatttgta
     1201 tattaacata aattttttac ttatgtaaga acctctcata tctatatctg gaaataaaga
     1261 acctaaatat gcaaaataaa tataaattat aattaatact attttatatg caatatcgta
     1321 tttaactagg tacagattgt ttataaaggg gagtatcaaa agtgcgaata tataacctcc
     1381 cctttcatgt gtttctttta tcatataaaa atcctcctga atcgtcacta taaacattat
     1441 attggtaaat ttagactatc ttttattata ttatatagca aacataagtt tttttcaaaa
     1501 ggattttaat atttaattaa tcttgagatt ttaaattttt gtaattttct aaagcaccat
     1561 tccaatttac tacattaaac cagttgtcaa tgtattcatt tcttctattt tgatatttta
     1621 aatagtaagc atgctcccaa acatcaagtc ctattatagg agttaggttt ttacttacag
     1681 ggctatcctg atttggagta gtcataatag ataatttccc atctttagta gctacaagcc
     1741 aagcccaacc agaaccaaag acatctaaag cagatttttg gaactcttgc ttaaattttt
     1801 caaaagaacc aaagtctcta tcaatagctt cttttaaaga ttcagaaggt atggtttttt
     1861 ctggcgtcat tatatcaaaa aagaatttat gattataagc tccacctgca ttatttctta
     1921 cagttgtagc aatatcttta ggtaaagaat ctaaattttg caataattca cataaagaat
     1981 aattataaag ctcaggatat ttttcaagag cagcatttaa tttatcaaca taagcttgat
     2041 aatgcttatc atgatgcagt ttcattgttt ctttatctat ataaggttca agtgcatcat
     2101 atgcataagg taatggttta accttaaatt tgttattttc aggtgtaaaa gcaaatgaag
     2161 ttatgcactg tgagattata aataaagaca taataactgg tattaatatt tttttcttca
     2221 taaaaatcac ctcctaatat agtatttcta gaaataaaaa aaatatgaaa tattaacaga
     2281 taatttataa gaatttaata gcaatttcat tttaaattga ataagtttta aagccaaaga
     2341 caaaactatg aaatatagaa taagactaaa ggacagaaag gaattttgat tatatggacg
     2401 gcaatatttc aatttattat aaaggtgata tatctaaaaa agaagatatt aaatttaaaa
     2461 taaaagaatc tgtaaataat acaagacttt ctagtagaat agagtataag gatgaggaaa
     2521 aaacttttga ttttgaaaga tttaatgtaa ttgatgctat ttatggaaat aaaccatttg
     2581 aaaaagatgg aaaagttatt gtttttaatg gaaatatata taactatcat gaaataaaag
     2641 aagaattaat taaaaaagga cacaatttta ctacaaatga tgatattgaa gtcttattgg
     2701 catcttatat agaatttggt aagaattgtg tacataagat taaaggaatg ttcaacttta
     2761 taatctatga tagagaaaat gaaagtatat ttggagcaag agatttattt gggataaaac
     2821 cactttatta tataaataag gaaaatgcaa taattttttc ctcagagtat aagtttttac
     2881 ttgaatatat gaaaaattta aatataaatg aaagaagctt acaaagttat ttttcatttc
     2941 aatatgtact tcctgaagat actatgatac aaggaataag gctgatacca gcaggtcact
     3001 attttagggt agaaaatggt atactatctt taaagagata taataaactt gagtttagaa
     3061 gtagtacaaa atttttttat actaaaaatc acttaggcaa tagagatgtg aatgaggatg
     3121 atataagaaa tatagtagta gattcaatag taactcatat ggaagaggag aaagaaattg
     3181 gaacattttt gtctggtgga atagattcat ctattataac tactgtggct tcacaaatta
     3241 atccaaatat aaaatctttt tctgcaggct ttagtgtcaa aggatatagt gaattagaag
     3301 tagcaaagaa aacagctgat aagttaggta tagaaaatat ccagattaat ataacacaag
     3361 atgaatatat aaaatcatta ccgaatgtaa tttattcttt ggatgaccct atagcagacc
     3421 cttcagaagt aggtctttat ttcttaatta aagaggcagg taaacatgtt aaagtagcat
     3481 tgtctggaga aggagcagat gaattatttg gaggatataa tatatataaa gagtatagta
     3541 ctatgaaatc agttgtcaat tcacctactt atattaaaag tatattagga agagtttcag
     3601 aacttatgcc aaacataaaa ggaagaaatt atctttatag agctacaaca ccattagaaa
     3661 aaagatatat aggaaatgca aaagtttttg aaaacagcga agtaaaaaga ttctttttta
     3721 agtataagga aaagaatata tatgaatacc tgttgtctaa tttgtataga gatgcacaaa
     3781 aaaataatta cgattatata agtaaaatgc aacatataga tgtgaataca tggcttcaag
     3841 gcgatatact tcaaaaaatt agcaaacttt ctacagctga gcaggttgaa cttagagtac
     3901 catttttata taaagatgta tttgatgtag caaaaaattt aagaatggag caaaaaataa
     3961 ataagaataa tacaaaagtt ttacttagag aggcgtttag agaaatagtc ccagaatatg
     4021 ttgttcaaag gaaaaaatta ggatttccaa ctccaataag agtttggtta aaagattctc
     4081 taggggatat tgtaaaggaa actatttata attcaaatgt agatgaattt atagataaaa
     4141 aatatgcaat aaaactttta gatacgcatc ttaaaggtca tagagataat tcgagaaaaa
     4201 tatggactat atttacattt tgtctatggc atcaattatt tattgagcat aaaaatgtcg
     4261 agtattaata cttttttcaa tattaatcag agggcaaaag catgcaatgt tttcaccctc
     4321 ttgattatta tggaatagga taagtagaat tttgattgat tagtttacta tctcaataaa
     4381 attattggca aatattttga caagattact ttcaacagct aaatttaagt tttgtccatc
     4441 atcatatcca gcggaaatca atcctagtaa gtttccaaac atatctaaaa gagctcctcc
     4501 tgaactacca ctagagattg gagctgtaaa ttgtatcatt tgaaccgttt caaaatcacg
     4561 aaaagctgag ataattccat ctgaaactgt attaaaaaga cctaaagggc ttccaattgc
     4621 aactattttt tgacctctca caggtttttt actgacttta acaggtattg gtttgcattt
     4681 tctatcaacc tttatcacag ctaaatcata atctgaatgg tattttataa tttggtaaga
     4741 tgtataaact ttatcatcat tttcaaatct aactaaaaat gaatacccat cattgactac
     4801 atgaagattc gtaagtatat aaccttcatt atttattact actccagaac cactcttaaa
     4861 cggtttattt tcgttattgc acacaacaat cataacaaca gaagctgata actttgcaag
     4921 gtcttcataa gaaagttcta cttcctcatc ttgtgcaatt tttttagatg ttagatttgt
     4981 gttattaagt gtatttttag agttaggttt attcgtagta ggagaagtct tattggtatt
     5041 ttcactgtaa tttatatttg taatatgcct ttcaaaaggt atttctaata tatttggagt
     5101 tttcctagaa agttcaaata gagtagaaaa ttttgaacat tcaggatata aatcaagaat
     5161 atctttattt ggatttatat ataaaatatc acacccaagt tgtgataaaa aatataagaa
     5221 aaaaagttct tgccttttaa tatttccaaa gtatactatc tttggtgaaa tagtagtctt
     5281 caccttgtta taaaataatt taggtagaaa atagtccatc caacacaaaa gtttaactgc
     5341 aaagtttttg atgatagagg atgtacaatt tggcatactt gtggaaaata aatctaaagt
     5401 atttttaaat gcaatctttt tagtccaatt taaagtgtca ttttctattt tacatggaaa
     5461 gtcgatatta tataaatccc aactgttaaa cttagaacta tcataaatat taaaaatttt
     5521 aagcatctta tctgtttgtt ctttgtcaaa atttattttt agattgttta atctaatata
     5581 gggaattgta ggattattaa aaaaataagt atctatattt ttaacttttt ctatgtattt
     5641 atcaattgaa tcatcaacac ctatatatct tgtaaagtgg acttctttaa tatatgaaga
     5701 actcttattt ttataggtag tatcagatat aaattcaatt agaggttcag ttgacttttt
     5761 tattgaaatc tttaatggtt taatagtaca aatcatattt tttctcctat aataagatga
     5821 tataatctat tataaaacaa cttaaaacaa tatagtacaa taatt
//
LOCUS       DAMJWP010000212         5844 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.219, whole genome shotgun sequence.
ACCESSION   DAMJWP010000212 DAMJWP010000000
VERSION     DAMJWP010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5844
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.219"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..120
                     /locus_tag="PC700_003106"
     CDS             <1..120
                     /locus_tag="PC700_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2582380.1"
                     /translation="DLSIREWICPSCNETHDRDINASINILKEGLRLITTQNK"
     gene            239..757
                     /locus_tag="PC700_003107"
                     /pseudo
     CDS             239..757
                     /locus_tag="PC700_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
     gene            784..2145
                     /gene="rlmD"
                     /locus_tag="PC700_003108"
     CDS             784..2145
                     /gene="rlmD"
                     /locus_tag="PC700_003108"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDF2582381.1"
                     /translation="MSVKKKDVIEFEVDKMEFGGTSLSQVGDRVIHMKGGISGQKVRA
                     GVKKVRSKKAEVKMIELLENSPLETEETCKHFRECGGCTLLSVPYEKQLEIKEKQVMD
                     LFLKQDLFGFQFLGIEGSPENKYYRNKMEYTFGDEVKNGPLTLGLHKKGKHIDIQTVE
                     ECMLVDEDFSKILVSSVEFFNEKKLPYYRTMNHKGYLRHLVVRKGIHTNEIMVNIVTS
                     SQEDFDMNEFKDMLLGIDLKAELVGVLHTINDGLADAVQCDELRVLYGRDYIQEEILG
                     LKFKISPFSFFQTNTKGAEVLYSIARDFIGDYNDKVVFDLYSGTGSIGQVMAGAAKKV
                     YGIEIVEEAVVAANENAKLNGLTNCEFIAGDVAKVVKDLKDKPDLIIVDPPRPGIHKD
                     AIRDICGFGAKEIVYISCNPKSLVVDLVDFKGYGYEIKMVKCMDMFPNTPHCETVVKL
                     IRE"
     gene            2469..2624
                     /locus_tag="PC700_003109"
     CDS             2469..2624
                     /locus_tag="PC700_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582382.1"
                     /translation="MFSKNEILKKLIEEYDVKTTTDIQDMLKDLFTNTIHQIFPLSNF
                     NGLNIIY"
     gene            2668..3702
                     /locus_tag="PC700_003110"
     CDS             2668..3702
                     /locus_tag="PC700_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH domain-containing protein"
                     /protein_id="HDF2582383.1"
                     /translation="MFKKKLIAVAMSITMISVGSFSYAHSGRTDSSGGHRDNKNKSGL
                     GSYHYHCGGYPAHLHNNGGCPYTGGGSSSGTTTSVNNEEKQKRSVGEKGYNQGYEDGY
                     KGNYSSSNYSGDYSDTYESKYSEGYEKGKAKLEEEEKVAKETGYNLGLTGAKSNNTYE
                     KEVLKNAYDTGYSTGYNEYKTKKIEEYKAKGIEDSNKDKEKMTFEENIDSEFIDAYNN
                     AYDEIQEQLKNDYTTQGFESAIKGERFDTSTIGNVKYANWFKEGYDNGKVKLPKAKES
                     VYNQGYNEEDFSIPYEMKSIETKLKGVYDEGLEKREEEKSRNATYGVGAGTAAVVAGV
                     VYKNKKRKKI"
     gene            3789..3992
                     /locus_tag="PC700_003111"
     CDS             3789..3992
                     /locus_tag="PC700_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HDF2582384.1"
                     /translation="MGFNFRKSINLGGGLKLNLGKKSVGISAGVKGARVSVNSKGRKS
                     ATLSIPGTGISYTKTSTKKRKPK"
     gene            4163..5323
                     /locus_tag="PC700_003112"
     CDS             4163..5323
                     /locus_tag="PC700_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp27"
                     /protein_id="HDF2582385.1"
                     /translation="MKLSKTLLAIGMSVSLLIASCPTSNALSSINSIKGTNKYETAGL
                     IADKQNYDIAILINSENGLSDGLSASGLSGAKNAPILLTKKDTIPSETLKRLEKVKEV
                     YLIGGKNSIGASVQKILSDKKISINRISGTDRIDTSHKVAKEIGRVSTVKKVILTNAF
                     KGEPDAMSVASIAVRDKSPIVLTDGNTTSFATDGLETYAIGGKVSMSDKLINETKATR
                     IGGVDRYDTNKQIINKFYNGSKEFYIASGTDLVYSLVGSTIAQKTPIVLVGNGSNKSI
                     LKNASKITAIGNIDDVIIKQCLNITNNVGDSNTGIVKDTNSNNTNKPSNEPSSNNPGL
                     TDKSTVYVTPNGKSYHLTKNCSTLKRSKTINKVTLKQAKSQGKIDPCNVCVK"
BASE COUNT         2316 a          583 c         1128 g         1817 t
ORIGIN      
        1 gacttaagta taagagaatg gatttgtcct agttgtaatg aaactcacga tagagatata
       61 aatgcaagta taaatatatt aaaagaagga ttaagactaa taacaactca aaataaataa
      121 cttatgtaag aaccgtagga actacgggga tagcctgtgg agaattagta agacatattt
      181 tagtatgcaa aattctgtga agcaggaacc ctgtgacttt agtcatggga ggttcaggat
      241 gaataaacct gatggatata tatcagcaac tacagataaa tatgacccaa cagtattaga
      301 tttaattgat ttatcatact tagcttttga accatttcct gttggaaggt tagataaaga
      361 cacagaggga ttattagttt taactaatga tggaaagctt tctcatagag ttttatctcc
      421 taagaagcat gtgcctaaga cttattatgc aaaaattgat ggagtagtta ctgaagaaga
      481 tgtagaagct tttttagaag gtgtagtgct tgatgacgga tataaaacta tgccatcaca
      541 gttaaatata ttaaaaagtg atgatgaatc agaaatagag cttacaatac atgaaggtaa
      601 gtttcaccaa gtaaaaagaa tgtttgaaag cgtaggcaaa aaagtagtat acttaaaaag
      661 attgtctatg ggcaatttaa aattggatga aagtttagag ttaggtgagt atagagagtt
      721 aacagatgaa gaagtaaaat tgattgaaga aagataataa agatactata gtaaattgga
      781 ggaatgtctg tgaagaaaaa agatgttata gaatttgaag tagataaaat ggaatttggt
      841 ggtacatctt taagtcaagt tggagataga gttatccata tgaagggtgg aataagtggt
      901 caaaaggtga gagctggagt taaaaaagtt agaagtaaga aagctgaagt taagatgata
      961 gaattattag agaactcacc acttgaaact gaagaaactt gcaagcactt tagagagtgt
     1021 ggaggatgta cactattgtc tgtgccatat gaaaaacaat tagaaataaa agaaaaacaa
     1081 gttatggatt tatttttaaa gcaagattta tttggatttc agtttttagg gatagaagga
     1141 agtccagaaa acaaatatta tagaaataag atggaatata cttttggaga tgaagtaaaa
     1201 aatggacctt tgacattagg tcttcataaa aaaggaaagc atatagatat acaaactgtt
     1261 gaggagtgta tgttggttga tgaagacttc tcaaagatat tagtttctag tgtagagttt
     1321 ttcaatgaaa aaaagcttcc ttattataga acaatgaatc acaaaggata tttgagacat
     1381 cttgtagtta gaaaagggat tcatactaat gagattatgg ttaatatagt aacttcttct
     1441 caagaagatt ttgatatgaa tgagtttaaa gatatgcttt tagggattga tttaaaggct
     1501 gagttagtag gtgtacttca tactattaat gatggtttag cagatgcagt ccaatgtgat
     1561 gagttaagag tattgtatgg aagagattat atacaagaag aaattttagg tcttaagttt
     1621 aagatttcac cattttcgtt tttccaaacc aatactaagg gagctgaggt tctttatagt
     1681 attgctaggg attttattgg agattacaat gataaggttg tatttgactt gtatagtggt
     1741 actgggtcta ttggacaggt tatggctggt gctgctaaga aagtgtatgg tattgagata
     1801 gttgaggaag ctgttgttgc tgctaatgag aatgctaagt tgaatggact tactaattgt
     1861 gagtttattg ctggggatgt ggctaaggtt gttaaggatt taaaggacaa gcctgatttg
     1921 attattgttg accctcctag accaggcatt cataaggatg ctattaggga tatttgtgga
     1981 tttggtgcta aggagattgt ttatatttct tgtaatccta agtctttggt tgttgatttg
     2041 gttgatttta aggggtatgg ttatgagatt aagatggtta agtgtatgga tatgttccca
     2101 aatacgccac attgtgagac tgtagttaaa cttattagag aataacaggg tttcagactt
     2161 ttatattgtg tcttaaaagt agatttgcca cctatttgcc accataatta tttttgggtg
     2221 gcaaatttat tctacaatga tttcaaaaat atttaccatc tggtttctca tgttattagt
     2281 tacatgtaaa tatgtatcca tagttgttga agttttagca tgtccgagta tatgctggat
     2341 atcttttata ttagtaccat tttctaataa catagtatca gggtttttaa aataaagtgt
     2401 gtattcttct agagtagtga taattaaaat atcaaatatc actatacaaa ggaaagtgca
     2461 ccctttttat gtttagcaaa aacgaaatat taaaaaaact tattgaagaa tatgatgtca
     2521 aaacaactac agatattcaa gatatgctaa aagatttatt tacaaataca attcatcaaa
     2581 tatttccatt gtcgaatttt aacggtttaa acataatata ttgactgata taataaaatc
     2641 atagaatatt ttagggggaa atagattatg tttaagaaaa agttaatagc tgttgcaatg
     2701 agtataacta tgattagtgt agggagcttt tcgtatgctc attcaggaag aactgattca
     2761 tcaggaggtc atcgtgataa taaaaataaa tctggattag gaagttatca ctatcattgt
     2821 ggtggttatc cagcccattt acataataat ggtggttgtc cttatactgg aggaggaagc
     2881 agttctggaa caacaacttc ggttaataat gaagaaaagc aaaagaggtc tgttggagaa
     2941 aaaggatata atcaagggta tgaagatggg tataaaggaa attatagttc atcaaattat
     3001 agtggagatt atagtgatac atatgaatca aagtactcag aaggatatga aaaaggtaag
     3061 gctaaattag aagaggaaga aaaggtggct aaagagactg gatataattt agggcttaca
     3121 ggtgctaaat ctaataatac atatgaaaaa gaagttttaa aaaatgctta tgataccgga
     3181 tattcaactg gatacaatga atataagact aaaaagatag aagaatataa agctaaaggg
     3241 atagaggata gcaataaaga taaagaaaaa atgacatttg aagaaaacat agattctgaa
     3301 tttatagatg cttataataa tgcttatgac gaaatacaag aacaattaaa aaatgattac
     3361 actactcaag gatttgagag tgctatcaaa ggagaaagat ttgacacatc aactataggt
     3421 aatgttaaat atgctaattg gtttaaagaa ggatatgata atggtaaggt taagttacct
     3481 aaagcaaaag aaagtgtata taatcaaggt tacaatgaag aagattttag tataccatat
     3541 gaaatgaagt ctattgaaac taagttaaaa ggtgtatatg atgaaggttt agaaaaaaga
     3601 gaagaagaga aaagtagaaa tgctacttat ggggttggag ctggaacagc agctgtagtt
     3661 gctggagtag tatataaaaa taaaaaacgt aaaaagatat agatgctaga tataccaatg
     3721 atgacaagtg tataacaaat ataattttaa gtataaatat ttaaaatata gaagggaagg
     3781 ttatatctat gggatttaac tttaggaaaa gcataaatct aggaggagga ctcaaattaa
     3841 atttaggtaa gaagagtgta ggtataagtg ctggtgttaa aggtgctaga gtgtcagtta
     3901 attcaaaagg aagaaaatca gctacattga gtatacctgg aactggaata tcatatacaa
     3961 aaactagtac aaagaaacgt aaacctaaat aaatatttga tatactaaga aatagactgt
     4021 tacaaatagt ctatttttta gtatatagat ttaaaaagtg acaaaatttt gaaatataaa
     4081 ctaatttcat aaaggaaaat attcttttat gtagaattat aatatttaag taatattata
     4141 aaatttatag gaggatttca ttatgaaatt aagcaaaaca ctattggcaa tagggatgtc
     4201 tgtttcatta ttgattgcta gttgtccaac atcaaacgct cttagttcta ttaattcaat
     4261 aaaaggtaca aataagtatg aaacagcagg tttaatagca gataaacaaa attatgatat
     4321 tgcaatactt attaattctg aaaatggatt atctgatgga ttgtcagcaa gtggattgtc
     4381 aggagctaaa aatgccccaa tattgttaac taagaaagat acaatccctt cagaaacatt
     4441 aaaaagatta gaaaaggtaa aagaagtata tctaattgga ggaaaaaatt cgataggagc
     4501 atcagttcag aaaatattaa gtgataaaaa aataagcata aatagaataa gtggaactga
     4561 tagaatagat acaagtcata aagttgcaaa agaaatagga agagtaagta cggttaaaaa
     4621 agtaatatta actaatgctt ttaaaggcga accagatgct atgagtgttg cttctatagc
     4681 agttagagat aaatctccaa tagtattaac tgatggaaat actacaagtt ttgctacaga
     4741 tggattagaa acttatgcaa ttggtggaaa agtcagtatg agtgataagt taataaatga
     4801 aactaaagca acaagaatag gtggagtaga tagatacgat acaaataaac aaataataaa
     4861 taaattttat aatggctcaa aagaatttta catagcaagt gggactgatt tagtttattc
     4921 tttagttggt tctacaattg cacaaaaaac acctatagtg ttagttggaa atggaagtaa
     4981 taagagtata ttaaaaaatg caagtaaaat aactgctatt ggaaatattg atgatgttat
     5041 tataaaacaa tgtttaaata taactaataa tgttggggat tcaaatacag gtattgtaaa
     5101 agatactaat tcaaataata caaacaaacc aagtaatgag cctagctcta ataatcctgg
     5161 attaactgat aaaagtacag tatatgtaac tcctaatgga aaatcatatc atctaacaaa
     5221 aaattgttct acactaaaaa gaagtaaaac aataaataaa gtaacattaa aacaagctaa
     5281 gtcacaaggt aaaatagacc cttgtaatgt ttgtgtaaaa taatgtatta gccaaatata
     5341 tattaaaaaa gacaattaat taagattgtc ttttttattg aagaaatata aatttatatt
     5401 atgtattatt aaaaagaaaa ttattaatta taaaaaataa taataaagac gataagtatt
     5461 aaaattttat gtttccaata tttatgatat aattaatatg gcaaggaata aatatttgaa
     5521 aaagctatga gtggtgtttc catggaagtt tcttcacttt cttatgagag gaggtgaaaa
     5581 ctatggaaaa tctaatgatg agtattataa ctggtgttat agccagttac atttacgata
     5641 aattaaagag ccactctgac caatgaacta tacccactgt tttatacagt taaaacatgt
     5701 aaaagtggtt ataaagtact agtgtcaatc attagtactt tttttattat attcatataa
     5761 ttaatagcta tattttaaag tttactatct aaaaaatagc acgtcaaata aaaccctaga
     5821 tatttaaatt tttttagagc tact
//
LOCUS       DAMJWP010000213         5811 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.220, whole genome shotgun sequence.
ACCESSION   DAMJWP010000213 DAMJWP010000000
VERSION     DAMJWP010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5811
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.220"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            175..1182
                     /gene="asrA"
                     /locus_tag="PC700_003113"
     CDS             175..1182
                     /gene="asrA"
                     /locus_tag="PC700_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrA"
                     /protein_id="HDF2582386.1"
                     /translation="MKLKLDSSKFNEGLNYLKKDYKIFAPVLIPFKGTFSDTDVIRYK
                     EVNAFEEMEFAKKANFSPKEVVLPINQVLFYFTEKEFKESDLDNKKILIFLRACDING
                     IKRLDEIYLNNGEEKDYFYKNIRDKVKFALVGCKESFRNCFCVSMDSNKTDNYSIGLN
                     IEGDTLYLDIKDSEFEVFNGEATEFNVDYVTDNLISVDVPENIDSNEIIGNSIWDEYD
                     TRCIGCGRCNFVCPTCSCFTMQDIFYKENENVGERRRVWASCQVDGYTDIAGGNSFRK
                     KQGERMRFKTMHKIHDFKKRFGYHMCVGCGRCDDACPQYISFSKCIEKINDLVISKEE
                     V"
     gene            1183..1974
                     /gene="asrB"
                     /locus_tag="PC700_003114"
     CDS             1183..1974
                     /gene="asrB"
                     /locus_tag="PC700_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HDF2582387.1"
                     /translation="MNPYIPVSAKILDIVKHTDKEWTFRVNCNTKGVLPGKFYEISIP
                     KYGESPISVSGYGEDYIDFTIRNVGKVTSELFNYKEGDSFFIRGPYGNGFDVSLYEGR
                     EIVVVAGGSALAPVRGIVEYFYNNKEKCEKFKLIVGFKSPKDILFADDLKRWSKKLDI
                     LVTVDGAEEGYSGNVGLVTKYIPELDMKDINNTSIVVVGPPMMMKFTVEEFLKRDLDE
                     KNIWVSYERKMCCGIGKCGHCKMDDTYICLDGPVFDYSYAKNLID"
     gene            1987..2958
                     /gene="asrC"
                     /locus_tag="PC700_003115"
     CDS             1987..2958
                     /gene="asrC"
                     /locus_tag="PC700_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HDF2582388.1"
                     /translation="MIRDLNTKKVMKNAYRITKTKYETSLRVRVPGGLIDPESLAVVS
                     KIASEYGNGQVHITTRQGFEILGINMEDMEEINKIIQPVIEKMNINQSEKNAGYPAAG
                     TRNIAACIGNKVCPKAQYNTTEFAKRIEKAIFPNDLHFKVALTGCPNDCIKARMNDFG
                     IIGMALPIYEKDRCVNCGACVKKCSKISVGALKTENNKVVRDKDKCIGCGECVLNCPT
                     NAWTRDEKKYYRLAIMGRTGKKNPRLAEDFLLWVDEDSIIKIILNTYRYVEKYIDKDA
                     PGGKEHIGYIIDRTGFMEFKKWALEGVELPEITKMYENIYWSGIKYL"
     gene            3015..3791
                     /locus_tag="PC700_003116"
     CDS             3015..3791
                     /locus_tag="PC700_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDF2582389.1"
                     /translation="MHKETLDKLTNAAINKINLLNTSKVKYLVSSAFAGLYVGIGILL
                     IFTIGGLLTDAGSPMTKIVMGLSFAIALSLVIMTGTELFTGNNMVMSAGMLNKGVSIK
                     DTSKIWAYSWVGNLIGALVLGLIFVGTGLVDKGPVAEFFANTAASKASMPFTALFFRG
                     ILCNILVCVSVLCSFRTNSDTAKIIMIFLCLFAFITSGFEHSVANMTIYSVSLFSPTI
                     STVTIGGAIYNLVAVTLGNIVGGALFMGLGTYILGKEKLN"
     gene            4062..>5811
                     /locus_tag="PC700_003117"
     CDS             4062..>5811
                     /locus_tag="PC700_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDF2582390.1"
                     /translation="MTKKLVISKTKLFIFIIAFISIFIYLFGSKNTLIGVGIVTAMLT
                     LLERDLTISPIKNLLKYLAINIILGILSFFAVQNMYLGVLLNFIALFIIGYVFSYDLK
                     RAVYVPFGLMYIFMVSIPVGKSEFPMRLSALAVGAVIIMIAQFVMNRNRMKNVGDKEL
                     ISICDELLEKISLLKNTINDDSSIIKSSMRKIDSCNYRINSISKNLKMVIFDNRKDDF
                     YISIRGIDIMNILFSLERISLIMERYKKDSKEFEDEDIKNILEESSINSKSDVLVVAT
                     KEINYIKMCLENKDTITDKEILGFRDYVIAQDTKNINLKEIYSVLENLYEFLVDYKKV
                     KSRDEKKAERKSKIPHEFKRLSIYKKNFNLNSIRFSYAVKIALATAVAGFIMDYFHLR
                     DGRWIMLTVFSLTQPYAENCIQRSRKRIEGTFIGAVIFIVLFSIIKDSTLRSLIVLLA
                     GYINSYVVDYRKLMVCVTVSALGSAVVMGDPNVLTISRISYVALGAIIALIVNKFILP
                     YDAKTGYQHVIEMYKGIVKNIIDEVNKSIENVADVYYIKNLLLIPSLIEDRLMLINAI
                     YKDKHQEDFLENQKLLI"
BASE COUNT         2327 a          518 c         1073 g         1893 t
ORIGIN      
        1 aaatcgaata atatagataa taaaaaaata ttaacagaga ttagtaaact ctattaatat
       61 ttttttatta tttttgaaac tgtgataatt atcacagaag catagaaaat aaagtgttaa
      121 gatttaaaca agatagcaag taagtaaaac taaaatataa gaaaggtgat aaaaatgaaa
      181 ctaaaattag attcatctaa gttcaatgaa ggtctgaatt atctaaaaaa agactacaag
      241 atatttgcac cagttttaat cccatttaaa ggtacttttt cagatactga tgttataaga
      301 tacaaggagg taaacgcttt tgaagaaatg gaatttgcta agaaagcaaa cttttcacca
      361 aaagaagttg tacttcccat aaatcaagta ttgttttatt ttactgaaaa agagtttaag
      421 gaaagcgatt tagacaacaa gaaaatttta attttcttaa gagcttgtga cataaatggt
      481 ataaaaagac ttgatgaaat atatctaaat aatggtgaag aaaaagatta tttttataaa
      541 aatatcagag ataaagtaaa gtttgcactt gtagggtgta aggaaagttt tagaaactgt
      601 ttttgtgtga gcatggattc taacaaaact gataattatt caattggatt aaatatagaa
      661 ggagatacac tgtacttaga tataaaggat agtgaatttg aagtatttaa tggagaagca
      721 actgaattta atgttgatta tgtaactgat aatttaatat ctgtagatgt gccagagaat
      781 attgattcaa atgaaattat aggaaattca atatgggatg aatatgatac aagatgtata
      841 ggatgtggta gatgtaattt tgtatgtcca acatgttcat gttttacaat gcaggatatt
      901 ttctataagg aaaacgaaaa tgtaggtgag agaagaaggg tttgggcttc ttgtcaagta
      961 gatggatata cagatatagc aggtggaaat tcttttagaa aaaaacaagg tgaaagaatg
     1021 agatttaaaa ctatgcataa aatacatgat tttaaaaaaa gattcggata tcatatgtgt
     1081 gttggttgtg gaagatgtga tgatgcctgt ccgcaataca tatcattttc aaagtgtata
     1141 gaaaaaataa atgatttagt aattagtaag gaggaagtat aaatgaaccc atatatacca
     1201 gtaagtgcaa aaatactaga tatagttaaa catactgata aagaatggac ttttagagta
     1261 aactgcaata caaaaggtgt attgcctgga aagttttatg agatatctat acctaaatac
     1321 ggtgaaagtc caatatcagt atcaggatat ggagaggatt atattgactt tacaataaga
     1381 aatgtaggaa aagtaactag cgagttgttt aattataaag aaggagattc tttttttata
     1441 agaggtcctt atggaaatgg atttgatgta tccctttatg aaggaagaga aattgtagta
     1501 gtggcaggag gaagtgcatt agcccctgta agaggaatag ttgaatattt ttataataat
     1561 aaagaaaaat gcgaaaagtt taaattaata gtaggattta aatcaccaaa agatatttta
     1621 tttgcagatg atttaaagag atggagtaaa aaacttgata ttttagttac tgtagatgga
     1681 gcagaggaag gatatagtgg taatgtagga ttagtaacaa aatacatacc agaattggat
     1741 atgaaagata taaacaatac ttctatagtt gtagttggac ctccaatgat gatgaaattt
     1801 acagtagaag aatttttaaa aagagattta gatgaaaaga acatttgggt ttcttatgaa
     1861 agaaaaatgt gttgtggaat aggtaaatgt ggacattgta aaatggatga tacttacata
     1921 tgtttagatg gaccagtatt tgattattca tatgctaaaa atcttattga ttagggggcg
     1981 aagtttgtga taagagattt aaataccaaa aaagtaatga aaaatgctta tagaattaca
     2041 aaaacaaaat atgagacttc tttaagagtt agagtacctg gtggtcttat tgaccctgag
     2101 agtttagctg tagtttctaa aattgcaagt gagtacggaa atggacaggt gcacataact
     2161 acaagacagg ggtttgaaat attaggaata aatatggaag atatggaaga aataaataaa
     2221 attatacaac ctgttattga aaaaatgaat ataaatcaaa gtgagaaaaa cgcaggttat
     2281 ccagcagcag gtacaagaaa tatagcagct tgtataggaa ataaagtctg tccaaaggct
     2341 caatataata caactgaatt tgctaaaaga atagaaaaag ctatatttcc aaatgattta
     2401 cattttaaag tggctttaac tggttgtcca aatgattgta taaaagctag aatgaatgac
     2461 tttggaatta ttggtatggc tttaccaata tatgaaaaag atagatgtgt aaattgtgga
     2521 gcatgtgtaa aaaaatgttc taaaatatct gttggagcat taaaaacaga aaataataag
     2581 gtggtaagag ataaagataa gtgtatagga tgtggagagt gtgtactaaa ttgcccaaca
     2641 aatgcatgga ctagagatga gaaaaaatac tatagattag ctataatggg tagaacagga
     2701 aagaaaaacc ctagactagc agaagatttt cttttatggg tagatgagga cagcataata
     2761 aagataatac ttaatactta tagatatgta gaaaagtata tagataaaga tgcaccagga
     2821 ggaaaagaac atataggata tataattgat agaacaggat ttatggaatt taaaaaatgg
     2881 gcacttgaag gtgtagagct tccagaaata actaaaatgt acgaaaacat atactggagt
     2941 ggaattaaat atctataaat tgagtaaaac atatatttga ataaattaaa atttaataga
     3001 aggaagaggg aattatgcat aaagaaactt tggataaatt aactaatgca gctataaata
     3061 agataaattt attaaacaca agtaaagtaa aatatttggt atcttctgcg tttgcaggac
     3121 tttatgtagg tataggtata cttttgatat ttactatagg aggacttttg actgatgcag
     3181 gttcacctat gactaagata gtaatggggt tatcatttgc aatagcacta agtttggtta
     3241 taatgacggg aacagagtta tttactggaa acaatatggt tatgtctgct ggtatgttaa
     3301 ataaaggtgt aagtataaaa gatacttcta aaatatgggc atacagttgg gtaggtaatt
     3361 taatcggagc tttggtttta ggtcttatat ttgtaggaac tggtctagta gacaaaggac
     3421 ctgtagctga attttttgca aatacagcag ctagtaaagc atccatgcca tttacagctc
     3481 ttttctttag agggatttta tgtaatattc tagtatgtgt atcagtgttg tgttcgttta
     3541 gaactaatag tgatactgca aaaataatta tgatattctt atgtttattt gcttttataa
     3601 catcaggttt tgagcatagt gtagcaaata tgacaattta tagtgtttca ttattttcac
     3661 caacaataag tacagttact attggaggag ctatttataa tttagtagct gtaacacttg
     3721 gaaatattgt tggaggagct ttatttatgg gtcttggaac ttatatatta ggtaaagaaa
     3781 agcttaatta aatttattgt ataaattata actctcttga taaatacatt tttagtatta
     3841 ttgagagagt tatttttata tttttgaaaa aatatttgaa acaaaaaaaa tatgggagca
     3901 tacgaaaata tatcggttat atataaaaaa aatacgttta tatatgaatt atatagttct
     3961 aaatataaaa aaagttaaag tcctaccgta aaaaagcgca ttttgaataa gataacaaat
     4021 atgataaaat tggtcttgtt gaaattttta ggggggatat gatgacaaag aaactagtaa
     4081 tatcaaaaac aaagttgttt atttttataa tagcatttat ttctatattt atatatttat
     4141 ttggaagtaa aaatacactt ataggtgtag gtatagtaac agctatgcta acactcttgg
     4201 aaagagattt aactatttct ccaattaaaa atttactgaa gtatttagca ataaatataa
     4261 tcttaggaat actatcattt tttgcagtac aaaatatgta tttaggagtt ttattaaatt
     4321 ttatagcatt atttataata gggtatgtat ttagttatga cttaaagaga gcagtatatg
     4381 taccttttgg tctaatgtat atatttatgg taagtatacc tgtaggaaaa agtgaatttc
     4441 ctatgagatt gtcggcttta gctgttggag cagttataat aatgattgct caatttgtta
     4501 tgaatagaaa taggatgaaa aatgtcggag ataaagaact gatatctata tgtgatgaat
     4561 tattagaaaa aattagtctt ttaaaaaata ctattaatga tgatagttct ataattaaat
     4621 ctagtatgag aaaaatagat agctgtaatt atagaataaa ttctatatct aaaaatttga
     4681 aaatggttat ttttgataat agaaaagatg acttttatat cagtattaga ggtatagaca
     4741 taatgaatat tttattttca ttagaaagaa taagtttaat catggaaaga tacaaaaagg
     4801 atagtaaaga atttgaagat gaagatataa aaaatatttt agaagaaagt agcataaata
     4861 gtaaatcaga tgttctagtt gtagcaacga aagaaataaa ctatattaag atgtgtttag
     4921 aaaataaaga taccataact gataaagaga tattaggatt tagagattat gttattgctc
     4981 aggatactaa aaatataaat ttaaaagaaa tttatagtgt tttagaaaat ttatatgagt
     5041 ttttagtaga ttataaaaaa gtaaaatcaa gagacgaaaa gaaagctgaa agaaaatcaa
     5101 agatacctca tgaatttaaa cgactatcaa tatataagaa aaactttaat ttaaattcta
     5161 taagattttc ttatgcagta aagattgctt tagctacagc tgtagcgggt tttattatgg
     5221 attatttcca tttaagagat ggaagatgga taatgttaac tgtcttttca ttaacacaac
     5281 cttacgcaga aaattgtata caaaggtcta gaaaaagaat agaaggtaca tttataggag
     5341 ctgtaatatt tattgtatta ttttcaataa taaaagatag tacattaagg tcgttaatag
     5401 ttcttttagc agggtatata aactcttatg tagttgatta tagaaaatta atggtatgtg
     5461 taactgtttc agctcttggt tcagcagtag ttatgggaga cccaaatgtt ttaaccataa
     5521 gcaggatttc atatgtagca ttaggagcaa ttattgcatt aatagtaaat aagtttatat
     5581 taccatatga tgctaaaaca ggctatcaac atgttataga aatgtataaa ggaatagtta
     5641 aaaatataat tgatgaagtc aataagtcta tagaaaatgt agctgatgtt tactatataa
     5701 aaaatttatt attgatacca tcacttattg aagatagact tatgttgata aatgctatat
     5761 ataaagataa acatcaagag gattttttag aaaatcagaa attactaata a
//
LOCUS       DAMJWP010000214         5757 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.221, whole genome shotgun sequence.
ACCESSION   DAMJWP010000214 DAMJWP010000000
VERSION     DAMJWP010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5757
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.221"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(241..2073)
                     /locus_tag="PC700_003118"
     CDS             complement(241..2073)
                     /locus_tag="PC700_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2582391.1"
                     /translation="MSRMGRGPMGKSMEAGQKANDFKGTMRKLIAYLSKFKISIILVI
                     VFAIGSASFSIVGPKILGKATTKIFEGLVSKVSGGNVGIDFNAIGKILTFLLFLYLIS
                     ALFSFIQGFIMSGISQKVSYNLRKEISAKLDRLPMKYFDTKTHGEILSRITNDIDTLN
                     QSLNQSMTQLITSVTTMIGVLIMMLSISGIMTLVAVLILPISMFVISRIIKKSQKYFR
                     YQQEYLGNVNGQVEETYSGQTIVKAFNREDEVIEEFDKLNDSLYNSAWKSQFLSGIMQ
                     PLMMFIGNLGYVMVSILGGWLAIKKTIEVGDIQSFIQYVRNFTQPMTQIAQVANLLQS
                     TAAASERVFEFLEEEEEVQIVENAVSIDGLEGKIDFENVNFGYNPNKTIINDFSVNVK
                     PGQKVAIVGPTGAGKTTIVKLLMRFYDVNSGSILIDGHNIKDFNRSELREMFGMVLQD
                     TWLFSGSIMENIRYGKLNATDEEVIEAAKSAHVHRFIKTLPDGYKMKLNEEASNVSQG
                     QKQLLTIARAILADPKILILDEATSSVDTRTEVLIQKAMDNLMEGRTSFVIAHRLSTI
                     RDADMILVMNEGDIVEQGNHEELLKKGGFYANLYNSQFEEDEAM"
     gene            complement(2066..4312)
                     /locus_tag="PC700_003119"
     CDS             complement(2066..4312)
                     /locus_tag="PC700_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2582392.1"
                     /translation="MFKLIKYLKKSALSIVIIVCLLVIQAVCDLSLPEYTSNIVNVGI
                     QQGGVENSVPSVIRESELNKITLFMDKSSKDKVLDNYTLLNKKDYVKYKDKYPGLKDE
                     SLYELNTKDKDTIDDLNVIFGKAILIVSGLEGDSKEVKAMKAQLMSKLPPQATQSGDV
                     DIFKLLSAMPKEQLDTLTKEMSKGFESMPESMITQSSVSYVRSEYEKIGIDTEKTQNN
                     YILFTGAKMLGIAMISMVATITVGFLAARVAASVGRNLRSGVFRKVMSFSNTEMNEFS
                     TASLITRSTNDIQQIQMLMVMLLRIVFYAPIMAIGGVIKVLNTNTSMAWIIAVAVVAI
                     LSLIVVLFSVVMPKFKLVQKLVDKLNLVSREIITGIPVIRAFSTEKHEEERFDKANKN
                     LTKTNLFVNRVMTCMMPAMMLIMNLITVLIVWRGAYSVDSGAMQVGDMMAFIQYTMQI
                     IMSFLMISMVSIMLPRASVSATRIDEVLVSDISIKDPEKSEAQEFKEDKKGLVEFKNV
                     SFMYPDAEEPILTNISFTAKPGETTAFIGSTGSGKSTLINLIPRFFDVTEGEILLGGV
                     NIKNVSQHDLREKIGYVPQKGVLFSGTIESNLKYGRKDATDEEMRTAAEIAQATEFID
                     SKPEAFKTEISQGGTNVSGGQKQRLSIARAIAKNPEVYIFDDSFSALDLKTDAALRKA
                     LKSKTAESTVLIVAQRISTILNAEQIIVLNEGEIVGIGTHKELLKNCEVYEQIALSQL
                     SKEELANE"
     gene            complement(4380..4841)
                     /locus_tag="PC700_003120"
     CDS             complement(4380..4841)
                     /locus_tag="PC700_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2582393.1"
                     /translation="MNSNSEEKLNDVFLQLMRIYFIRTHFYFDKLGLYHGQPQILFSL
                     KMKDGQSQKELADKRKVKASTTTVMIKRMEKAGLVERKQDEKDQRVSRIFLTDKGLEI
                     CNKVEEFNKELEREFFLGFSEEEKIVLRRLLMQVRDNLKAGCHKHEDEKYF"
     gene            complement(5146..5712)
                     /locus_tag="PC700_003121"
     CDS             complement(5146..5712)
                     /locus_tag="PC700_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HDF2582394.1"
                     /translation="MRKLEEILEYNKSFVENKEYEQYVTSKHPNKKIVVLSCMDTRLT
                     ELLPKAMNLKNGDVKLIKNAGATIMHPFGSIMRSVLVAIYEFDVDEVMVVGHHGCGMC
                     NVDTDKLLGKILDRGISNDVILTLRSAGIDTKQWLHGFDSAEESVKDSIDLIKGHPLI
                     PDGIIVHGLIISPETGKLDVVVNGYEDK"
BASE COUNT         1829 a         1076 c          625 g         2227 t
ORIGIN      
        1 ttgataatca cctccaacat tttccttact acatattata ataaaataaa cactttgtaa
       61 agacataaaa ttatgtggct atctcaaaat atagataatc ctctttttca ttgttaaaat
      121 gataaaaata cactttattt catacacaaa agagtgcctt atgaattaaa aagttcattt
      181 taaggcaccc tcatttattt tatcttatta attacatgtc tatgctaaga tagttttttg
      241 ttacattgct tcatcttctt caaattgaga attgtaaaga ttggcataga atccgccttt
      301 ctttagaagt tcctcatgat ttccttgttc aactatatct ccttcattca ttacaagaat
      361 catatcagca tcacgtatag ttgataatct gtgggctata acaaaacttg ttctaccttc
      421 cataagatta tccatagctt tttgaatcaa tacttctgtt ctagtatcga cagaacttgt
      481 agcttcatct aatattaaaa ttttaggatc agctaaaata gcacgagcta tagtcaataa
      541 ttgtttttga ccttgagaaa cattacttgc ttcttcattt aatttcatct tatatccatc
      601 tggtaacgtc ttaataaatc tatgaacatg tgcagattta gcagcctcta taacttcttc
      661 atctgtagca ttcaacttac catatcttat attttccata attgaaccac taaataacca
      721 tgtatcttga agaaccatac caaacatttc tctaagctca ctacgattaa aatctttaat
      781 attatgacca tcaatcagta ttgaaccgct attaacatca taaaatctca ttaacagttt
      841 aactattgta gttttacctg ctcctgtagg tccaacaata gcaacttttt gaccaggttt
      901 aacatttact gagaaatcat ttattatagt cttgtttggg ttatatccaa agttgacatt
      961 ttcaaaatca attttacctt ctaaaccatc tattgaaact gcattttcaa caatttggac
     1021 ttcttcttcc tcttctaaaa actcaaatac tctttcagaa gctgctgctg ttgattgcaa
     1081 taaattggca acctgtgcaa tctgagtcat tggctgtgta aagtttctta catattgtat
     1141 aaatgactgt atgtcaccaa cctcaattgt tttcttaatt gcaagccaac ctcctaagat
     1201 tgataccatt acatatccca agtttccaat aaacatcata agtggttgca ttatacctga
     1261 taaaaattga gatttccacg ctgaattata caggctatca tttagtttat caaactcttc
     1321 aattacttca tcttcacgat taaatgcttt cactattgtt tggccactat aagtttcctc
     1381 aacttgacca tttacatttc ctaaatattc ttgttgatat ctaaagtatt tttgtgattt
     1441 ctttattatt cttgaaatta caaacattga tattggtaat attaaaactg ccactaatgt
     1501 cataattcca cttatagata acatcattat caaaacacct atcatagttg ttactgatgt
     1561 tataagctgt gtcatacttt gatttaaact ttgatttaaa gtatctatat cattcgtaat
     1621 tctcgataat atttcaccat gcgtcttagt atcaaaatac ttcattggta atctatccaa
     1681 ttttgctgaa atctcttttc ttaaattata ggatactttt tgagaaattc cactcattat
     1741 aaatccttgt ataaaagaga acagtgcact tatcaaataa aggaaaagta aaaaggttaa
     1801 gatttttcca attgcattaa aatctatacc tacgtttcct ccagaaactt tactcactaa
     1861 cccttcaaat atctttgtag ttgcctttcc taaaattttt ggacctacta tagaaaacga
     1921 tgcactacca atagcaaata caattactaa aattattgat attttaaatt tagataaata
     1981 tgcaattagt tttcgcatag tccctttaaa gtcatttgct ttttgtcctg cttccataga
     2041 ttttcccata ggacctcttc ccattctact cattagctag ctcctccttt gaaagttgtg
     2101 ataaagctat ttgctcataa acttcgcaat tttttagaag ctctttatgt gttcctattc
     2161 caactatttc tccctcattt aaaacaatta tctgttctgc atttaagata gtactaattc
     2221 tttgtgcaac tattagaact gtactttcag cagtttttga ttttaaagcc ttacgaagag
     2281 ctgcatcagt ttttaagtca agtgctgaaa aactatcatc aaatatataa acttctggat
     2341 tttttgctat tgctcttgca atagaaagtc tttgtttttg acctccagat acatttgttc
     2401 caccttgaga aatttcagtt ttaaaagctt ctggtttaga atctataaat tctgtagctt
     2461 gagctatttc agctgctgtt ctcatttctt catctgttgc gtcttttcta ccatatttta
     2521 agttggattc tatagtacca gaaaacaata ctcccttttg aggtacatat ccaatttttt
     2581 cccttaaatc atgttgagaa acatttttta tattaactcc accaagtaat atttctcctt
     2641 cagtaacatc aaagaaacga ggaattaaat ttataagagt tgacttacca ctaccagtac
     2701 ttcctataaa ggcagttgtt tctcctggct ttgctgtaaa agatatattt gttaatatag
     2761 gttcttctgc gtctggatac atgaacgaaa catttttaaa ttcaacaaga ccctttttat
     2821 cttccttaaa ctcttgtgcc tctgattttt ctgggtcttt tatgcttata tctgacacta
     2881 atacttcatc tatacgtgta gctgaaacgg atgctcttgg aagcataata gataccattg
     2941 atatcattaa gaatgacatt attatttgca ttgtatattg tataaatgcc atcatatctc
     3001 caacttgcat tgctccagaa tcaacactgt atgcacctct ccaaactata agaactgtga
     3061 taaggttcat tataagcatc atagcaggca tcatacatgt cataactcta tttacaaaaa
     3121 gatttgtttt agttaaattt ttatttgctt tatcaaatct ttcttcttca tgtttttcag
     3181 tactaaaagc tctgataact ggaataccag taattatttc acggcttact aagtttaact
     3241 tatcaacaag cttttgaacc agtttaaact taggcataac cactgagaat aacactacaa
     3301 ttaatgataa gatagcaact actgctactg ctataatcca ggccattgaa gtatttgtgt
     3361 ttaaaacctt tatcactcca cctatagcca ttataggtgc atagaatact attctaagca
     3421 acataaccat caacatttgt atctgttgaa tatcatttgt actacgagta attaatgaag
     3481 ctgtagaaaa ttcattcatt tcagtatttg aaaatgacat aacttttcta aatactccac
     3541 ttcttaaatt tctacctaca cttgctgcta ctcttgctgc taaaaatcct actgttattg
     3601 tagctaccat actaatcatt gctataccaa gcattttagc accagtaaat aatatataat
     3661 tattttgagt tttttctgta tcaataccta ttttctcata ttctgacctt acatatgaaa
     3721 cagagctttg tgttatcata ctctctggca tactttcaaa gcctttgctc atctctttag
     3781 tcaatgtatc taactgttcc tttggcattg ctgataacaa tttaaatata tcaacatctc
     3841 cactttgagt agcttgtggt ggaagtttac tcattaactg agctttcata gcttttacct
     3901 ctttactatc gccctcaagc cctgatacta ttaaaatagc tttaccaaaa ataacattta
     3961 aatcatctat agtgtctttg tctttagtat ttaactcata aagagattca tcttttagac
     4021 ctgggtactt atctttatac ttaacataat cctttttatt cagcaaagta taattatcta
     4081 gtactttatc cttactactt ttatccataa ataaagtaat tttatttaat tcactctctc
     4141 taatcacaga tggaacagag ttttctactc caccttgttg tatacctaca tttactatat
     4201 tggatgtgta ttctggtaaa gaaaggtcac ataccgcttg aattaccaat agacagacta
     4261 ttattactat tgataatgcc gatttcttta aatatttaat taatttaaac atacatttct
     4321 tcccttctgt aatattaatt agtctaataa cattaaattg aaaaacatta ttttaaactt
     4381 taaaaatatt tttcatcttc atgcttatga catcctgctt ttaaattatc tcgtacttgc
     4441 attagaagtc ttctaagtac tattttttct tcttcactaa atcctaaaaa aaactctctt
     4501 tccaattcct tgttaaattc ttcaacttta ttacatattt ctaaaccttt gtctgtcaaa
     4561 aaaatcctag atactctttg gtccttttca tcttgttttc tttccacaag tccagctttt
     4621 tccatcctct ttatcataac tgtagttgta gatgctttta ctttcctttt atctgctaat
     4681 tctttttgac tttgcccatc tttcatcttt aaagaaaaaa gtatttgggg ttgaccatga
     4741 tataagccta atttatcaaa ataaaaatga gttcttataa aatatattcg cattaattgc
     4801 aagaaaacat catttaattt ttcttcagaa ttgctattca tacttgccct ccttattttt
     4861 tatattactt agtcggctaa gcattatttt actatactgc atgcattttt tcaatatatt
     4921 tataaaaaat tcatataaat ttcatttatt aaatattttt atatcatata gttataattt
     4981 taataaataa tagtaagatt tttaattagt cagctactta tattttttat tgtaaagctt
     5041 aaaataaacc ttttcatata ttttcaaata aaaaagttgt cccaaaataa attttgaaac
     5101 aacatcatat tcaacagtat cttgattata taacattaca atactctact tatcttcata
     5161 tccatttaca acaacgtcta acttcccagt ttcaggtgat ataattagac catgtacaat
     5221 aataccatct ggaattaaag gatgcccttt tattaaatca atactatctt ttacagattc
     5281 ttctgcagaa tcgaatccat gtaaccattg ttttgtatca attccagcac tacgaagtgt
     5341 aagtattaca tcattagata ttcctctatc taaaatttta ccaagtaact tatctgtatc
     5401 aacattacac ataccacatc catgatgccc tacaaccata acctcatcta catcaaattc
     5461 gtaaattgca actaaaacac ttctcataat acttccaaaa ggatgcatta tagtagctcc
     5521 tgcatttttt attagtttca catctccatt tttcaaattc atagccttag gcaaaagctc
     5581 agttaatctt gtatccatac aagataagac tactattttt ttatttggat gttttgatgt
     5641 cacatattgt tcatattctt tgttctctac aaatgattta ttatattcta atatctcttc
     5701 aagttttctc atatgtaata tcaccctctt taaaaatttt tttattatta tatcact
//
LOCUS       DAMJWP010000215         5753 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.222, whole genome shotgun sequence.
ACCESSION   DAMJWP010000215 DAMJWP010000000
VERSION     DAMJWP010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5753
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.222"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            57..2288
                     /gene="pflB"
                     /locus_tag="PC700_003122"
     CDS             57..2288
                     /gene="pflB"
                     /locus_tag="PC700_003122"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDF2582395.1"
                     /translation="MNAWQGFKTGRWTKEINVREFIQLNYSPYEGNDSFLAGATENTK
                     KLWDEAMVLFKKERENGGTLDVDTKTVSGIAAYAPGYLDKELETIVGLQTDAPLKRAV
                     MPYGGIKMVENSCEAFGYELDPEIKDIFTKYRKTHNQGVFDVYTPEMRAARKSGIITG
                     LPDAYGRGRIIGDYRRVALYGVDALIEDKNEQKKSLEVSCMDEEVIRLREEITEQISA
                     LNELKKMAESYGFDISKPATNSKEAVQWLYFGYLGAVKDQNGAAMSLGRTSTFLDIYF
                     ERDLKAGIVTEEELQEYMDHFVMKLRMVKFLRTPDYNNLFSGDPTWVTECIGGMGIDG
                     RTLVTKNSFRMLNTLYTLGPSPEPNLTVLWSTKLPQGFKDFCSKVSIDTSSVQYENDD
                     LMRAYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKTLLYAINGGVDEKSGVQVGPRF
                     EPITSEYLDYDEVMSKFEPFTDWLATLYVNTLNVIHYMHDKYSYEALEMALHDRDIFR
                     TMACGMAGLSVCADSLSAIKHAKVKTIRNEQGIAVDFEIEGDYPKYGNNDDRVDSIAV
                     ELVESFMNKIRKNKTYRNSYPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPM
                     HGRDTNGALASLSSVAKLPYEHAQDGISNTFSIVPAALGKDMTERINNLSAMMDGYFS
                     QNAHHLNVNVFDRATLEDAMEHPEEYPQLTIRVSGYAVNFIKLTKEQQLDVINRTFHG
                     KMA"
     gene            2448..3182
                     /gene="pflA"
                     /locus_tag="PC700_003123"
     CDS             2448..3182
                     /gene="pflA"
                     /locus_tag="PC700_003123"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HDF2582396.1"
                     /translation="MIKGKIHSIETFGTVDGPGIRYILFFQGCPLRCKYCHNRDTWDI
                     KSGKEYTVDEIITDALKYTSFMKFSGGGITASGGESTLQPEFLSELFKKAKENDIHTC
                     LDTSGFVDIETIDPVLDNTDLVLLDLKHMVEEKSIDLTGVGMDKALKLARHLESRNIP
                     VWIRHVLVPGITDDIDNLEKLGQFVATLKNVERFELLPYHSMGIHKWESLGIDYELKD
                     VPDATKEDVQKASEIISKFGVKVYNS"
     gene            complement(3304..4974)
                     /locus_tag="PC700_003124"
     CDS             complement(3304..4974)
                     /locus_tag="PC700_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF and EAL domain-containing protein"
                     /protein_id="HDF2582397.1"
                     /translation="MREESNSLSLDHIEIFFELLSQSTEDYIFFWDINRNKFKISSAI
                     FDEFNLSKEIESDLVNCWSKIVYPDDVQIWKDDIQELLHGKKGEHNLEYRLINKYEEI
                     VWISCRGKVYVSDDPKTIFLVGRIKNIGEKNKFDSITGTWNREQFEHRMNYLIKEKIY
                     KNGAMFIMDIDNFKNINEKYGHSYGDKVLRAIATEVLEYLPKDVRLYRLDGDEFAFFY
                     PMCTKETIEKIYEKIQMYTNTQHEIESNKYYCTVTAGVAMYPEDGDNYLDLFKHADIA
                     LDIAKISGKNRIKFFSQELYENKLKVISMQQKLRECVENNFNDFELFFQPQVNAVTKE
                     VIGAEVLLRWHSSTYGEVSPVEFIPILEQSNLIIPVGKWIIKEAVKQCKEWHKINPDF
                     KISVNVSYIQLKEDFFRDFIVECLVEYQLRPEFLILELTENCWIPDINLLNDKFISLK
                     GIGVYIAIDDFGTGYSSLNYLKELSVNIIKIERSFVKNITYNSYEYTFLEYIIKLAHI
                     INLKVCVEGIESYEEYDIVKSLGVDIIQGFLFGRPVSASEFYKFQLSK"
BASE COUNT         2028 a          829 c          831 g         2065 t
ORIGIN      
        1 taaaaaataa ttttaatagt attatttatc atttaaatct aaggaggaaa tacttaatga
       61 atgcatggca aggatttaaa acaggaagat ggactaaaga aataaatgtt agagagttta
      121 tacaattaaa ctattctcca tatgagggaa atgactcttt cttagctggt gccactgaaa
      181 atactaaaaa attatgggac gaagcaatgg tcctatttaa aaaagaaaga gaaaatggtg
      241 gtactttaga tgttgataca aaaactgttt ctggaatagc tgcatatgct ccaggatatt
      301 tagataaaga attagaaaca atagttgggt tacaaacgga tgccccttta aaaagagctg
      361 tcatgccata tggtggtata aagatggttg aaaattcatg cgaagctttt ggatatgaat
      421 tagacccaga aataaaagat atatttacta aatacagaaa aacacacaac caaggagttt
      481 ttgatgttta tactccagaa atgagagctg ctagaaaatc tggtataata acaggtcttc
      541 cagatgctta tggtagaggt agaataattg gtgactacag aagagttgct ctatacggtg
      601 ttgatgcttt aatagaagat aaaaatgagc aaaagaaatc attagaagtt tcttgcatgg
      661 atgaggaagt tatcagatta agagaagaaa taactgagca aatatcagct ttaaatgaac
      721 ttaaaaaaat ggctgaatct tatggatttg atatatcaaa accagcaact aactcaaaag
      781 aagctgtaca atggttatac tttggttatt taggtgctgt taaagaccaa aatggtgctg
      841 caatgtcttt aggtagaaca tctactttct tagatatata ctttgaaaga gatttaaaag
      901 ctggaatcgt aacagaagaa gaattacaag agtatatgga ccattttgtt atgaaattaa
      961 gaatggttaa gttcctaaga actcctgatt acaataacct attctctgga gacccaactt
     1021 gggtaactga gtgtatagga ggtatgggaa tagatggtag aacattagtt actaaaaact
     1081 catttagaat gttaaatact ctatatacat taggaccttc tccagagcca aacttaacag
     1141 ttctatggtc aactaaatta cctcaaggat tcaaagattt ctgttctaaa gtatctatag
     1201 atacaagttc agtacagtat gaaaatgatg atttaatgag agcatactgg ggagatgact
     1261 atggtatagc ttgttgtgta tctgcaatga gaattggtaa acaaatgcag ttctttggag
     1321 ctagagttaa cttagctaaa actttattat acgctataaa tggtggtgta gatgagaaat
     1381 ctggtgttca agttggacca agatttgaac ctataacttc tgagtactta gattatgatg
     1441 aagttatgag taaatttgaa ccattcactg attggttagc aacattatat gtaaatactt
     1501 taaatgtaat acattatatg catgataaat attcttatga agcattagaa atggcattac
     1561 atgatagaga tatatttaga actatggctt gtggtatggc tgggttatca gtttgtgcag
     1621 actcattatc tgctataaaa catgctaagg ttaaaactat tagaaatgaa caaggaatag
     1681 ctgttgattt tgaaatagaa ggagattatc caaaatacgg aaacaatgat gatagagttg
     1741 atagtatagc tgtagagtta gttgaaagct ttatgaataa aataagaaaa aataaaactt
     1801 atagaaattc ttatccaact caatctatac ttacaataac ttcaaatgtt gtttatggta
     1861 agaaaactgg taatacacca gatggtagaa gagctggtgc tccatttgct cctggtgcta
     1921 acccaatgca tggtagagat actaatggtg ctttagcttc attatcttca gttgctaaat
     1981 taccatatga acatgctcaa gatggtatat ctaatacttt ctctatagta cctgctgctt
     2041 taggaaaaga tatgactgaa agaataaaca atctttctgc aatgatggat ggatacttct
     2101 ctcaaaatgc tcatcactta aatgtaaatg tatttgatag agctacttta gaagatgcta
     2161 tggaacatcc agaagaatat ccacaattaa ctataagagt atctggatat gcagttaact
     2221 tcataaaatt aactaaagag caacaattag atgttataaa cagaacattc cacggtaaaa
     2281 tggcttagtc tttaaatttt tatactttaa ctaagattac tactgtgtaa aaaataaaaa
     2341 taaaaggcta tcttatattt gaattatttc tggtataaga tggccttttt tagacaaata
     2401 ttataactat tgataaggtt attaaaatat attgtgaggt gaactctatg attaagggaa
     2461 agatacattc tatagaaacg tttggtactg tagatggacc aggaataaga tatatattat
     2521 tttttcaggg gtgtcctctt agatgcaaat actgtcataa cagggataca tgggatatta
     2581 aatcaggaaa agaatacaca gttgatgaaa taataacgga tgctcttaag tatacttctt
     2641 ttatgaagtt ttctggtgga ggtataactg cctctggtgg tgaatccact cttcaaccag
     2701 aatttttaag tgaattattt aaaaaagcaa aagaaaatga tatacacact tgcttggata
     2761 catctggatt cgtagatata gaaactatag acccagtttt agataatact gatttggttt
     2821 tacttgattt aaaacatatg gttgaggaaa aatctattga tttgactggt gttggtatgg
     2881 ataaggcatt aaaacttgct agacacttag aatcaagaaa tattcctgta tggataagac
     2941 atgttttagt tcctggcatt actgatgata tagataattt agaaaaacta ggtcaatttg
     3001 tagctacatt gaaaaatgta gaaagatttg aattgctacc ataccattct atgggaatac
     3061 acaaatggga aagtttaggt atcgattatg aactaaaaga tgttcctgat gccactaagg
     3121 aagatgtcca aaaagctagt gaaataatct ctaaatttgg agtgaaagta tataatagtt
     3181 agtgtagtat attaatcctt tatagaagac ataacaaaat agggtaaagt gtaaattttt
     3241 aataaacttc aaaacttcca ctttgcccta tttttatata ctatatatct cattttatgt
     3301 acatcacttt gatagttgaa atttataaaa ttcagaggca cttacaggtc ttccaaataa
     3361 aaaaccctga ataatatcta cacctaagct ttttactata tcatactctt cataagactc
     3421 tattccctca acacaaactt ttaaatttat aatatgagct aatttaataa tatattcaag
     3481 aaaagtatat tcataactat tatatgtaat attcttaaca aaacttcttt caatttttat
     3541 tatattaaca gacagttctt taaggtaatt taaagatgaa taccctgtac caaagtcatc
     3601 tattgcaata tatactccta taccctttaa actaataaat ttatcattta gtaaatttat
     3661 atctggtatc caacaattct ctgttaattc caaaattaaa aattcaggtc ttaattgata
     3721 ctctactaaa cattctacta taaaatctct aaaaaaatct tcttttaact gaatatacga
     3781 tacatttaca gatattttaa agtctggatt tattttatgc cattctttac actgttttac
     3841 agcttctttt ataatccact tcccaactgg tataattaaa ttactctgtt ctagtattgg
     3901 tataaattca acaggagata cttctccata agtactcgaa tgccatctta aaagtacctc
     3961 tgcccctatt acctcttttg taacagcatt tacctggggt tgaaaaaaca gctcaaagtc
     4021 attaaaatta ttttcaacgc actctcttag tttttgttgc attgaaatta cctttagttt
     4081 attttcatag agctcttgtg aaaaaaactt aattctattt ttaccactaa tttttgctat
     4141 atccaatgca atatcagcat gtttaaataa atctaaataa ttatctccat cttctggata
     4201 catagcaact cctgcagtca ctgtgcaata gtacttattt gattcaattt catgctgagt
     4261 atttgtatac atctgtatct tttcatatat tttctctata gtttctttag tacacatagg
     4321 gtaaaaaaat gcaaattcat ctccatctaa cctatacaat cttacatctt ttggaagata
     4381 ttctaatact tcagtagcaa ttgcacgcaa tactttgtca ccataagaat gtccatattt
     4441 ttcatttata tttttaaaat tatcaatatc catgataaac atagcaccat ttttataaat
     4501 tttctctttt ataagataat tcattctatg ttcaaattgt tctctattcc acgttcctgt
     4561 gatagaatca aatttatttt tttcaccaat attctttatt ctcccgacta gaaatatagt
     4621 ttttgggtca tccgacacat aaactttacc tctacaagaa atccatacta tttcttcata
     4681 tttattaatc aacctatatt ctaaattgtg ctcacctttt tttccatgta gtaattcttg
     4741 tatatcatct ttccatattt gaacatcatc cggataaaca attttactcc aacaattgac
     4801 taaatcagat tctatctctt tacttaagtt aaattcatca aaaattgctg aagaaatttt
     4861 aaatttattc ctatttatat cccaaaaaaa tatataatct tctgtacttt gagataaaag
     4921 ttcaaagaaa atttctatat ggtctaaact taaagagttc gattcttctc tcatcttttc
     4981 cccctacaat attactatta gtgtagttta atcaacaaaa tattcttatt tagattatac
     5041 tattttacta atttttttat ctcatcaaac acatttcccc aactttagtt atcttatcaa
     5101 atctcttact cttaactaag tttttttgag attcataaag taatttttct acaatcatta
     5161 ctggtgattt attttcacct gctaaaacac catgttaaat catttattaa atcattttct
     5221 cttaaaagtc cattttttat tatataaaga gttctcagtt ctttattcta taaagctttt
     5281 ctattactct tgaatttgtt ttattttttc tcagcctatt ctctttggat ttggtattgc
     5341 ttttctatat attttggcaa agaagtgcct cttcctataa tataagattt tgatatttct
     5401 tcatcatcta taaatttttt aatagggatt atatcatttt ttatatttat ttatataaat
     5461 attttttgct tttaattttt tatctcttat tatgtactta catttaattt attcttttca
     5521 gttaataaaa taggtacatt tttagtacat gaaaatagag ttgcttacag ggtatctagg
     5581 attgatgtac tatttactag tattacattt tcagccgatt caacccctct tttgcttaca
     5641 taatttaagt atatttttct ggatttttaa atttatgtac tttaaaatgg atatccttat
     5701 tttcattgat tttataagaa ttaataaaaa atacgattac actacagaat tag
//
LOCUS       DAMJWP010000216         5686 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.223, whole genome shotgun sequence.
ACCESSION   DAMJWP010000216 DAMJWP010000000
VERSION     DAMJWP010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5686
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.223"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            197..1465
                     /locus_tag="PC700_003125"
     CDS             197..1465
                     /locus_tag="PC700_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDF2582398.1"
                     /translation="MEASIHTIASNNLLIIFAIVSITGIICSKLSEIIKVPDVVLYLL
                     VGILIGPSFLKFIDIRGFQIENQLILTFGSAFILYLGGKEISLKVLRNVKISVFLLAT
                     LGVVISAFIMQQVIGFTFGISAMTALLAGTIIASTDPATLVPIFNQVKIKDRVKQTVI
                     SESAFNDATGAILTSAVLAVILSNKFSLGENIYELGTMVIVGVLVGLITGILLLKLVN
                     DKPYGIFKDFAPIISVISVVVAYELSTKLGGSGYMSCFIVGIITGNKKNFKIWLSQKS
                     YDADFYVAETLGTICRMAIFIILGSQVNLAQLSKYFMPSVITVLVLMFIARPLCVLVC
                     TLVDREAKWSKSEILFMMWVRETGVIPAALCGIISAMKVPGYEVISSVVFMTILITLV
                     IQGSTTKLVAKKLGLLEEEVINISEKVSTF"
     gene            1660..2448
                     /locus_tag="PC700_003126"
     CDS             1660..2448
                     /locus_tag="PC700_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HDF2582399.1"
                     /translation="MFTVMDIVQPDTVEEAYSILNKRKTNQVIGGSAFLRMGKKRIGT
                     GIELSKLNLDYIKEYEDYVEIGSMTTFRTLETSSIIKNNFGRIIEDSVKNIIGVQFRN
                     VVTVGATVFSKYGFSDLIVALLSLDTEVELYNIGRISLEEFLNRDYEKDLLIKIYIKK
                     TNKNASYKSLRNAKSDYPILNVSVSKHMEQFKLCVGARPQKATIAKQASEFLSNNEIN
                     EINIDKAVEIASEELTFGSNMRASREYRKAMSKVLLKRAIMEVI"
     gene            2448..2897
                     /locus_tag="PC700_003127"
     CDS             2448..2897
                     /locus_tag="PC700_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HDF2582400.1"
                     /translation="MLVKININGKKKSVDIEPEEYLVDTLRKVGNLSVKRGCDTGCCG
                     LCTVLIDKKPTLSCATLTLRALDKEITTIEGLENEVKEFSEVLVKEGAEQCGFCSPGF
                     ILTVLAMKDELENPTDDEIKHYLTGNLCRCTGYMGQLRAIKTYLEVK"
     gene            2901..5165
                     /locus_tag="PC700_003128"
     CDS             2901..5165
                     /locus_tag="PC700_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2582401.1"
                     /translation="MNIVGKGIKKIDGMSITTGKPVYTDDLSAKNALVVKILRSPHAY
                     AKIKDIDTSKAEMVDGVECVLTYKDVPKSRFTLAGQTYPEPSPYDRLILEDVVRYVGD
                     EVAIVAAIDEKTAIKAMNLIKVKYEVLTPVLDFEEAIGNKTMVHDEDRHCNFDIGMQR
                     EKNIVSHHDYTKGDVEKVLSNCDVVIEETYYTQAQAHAMMETYRAYNYLDHMGRLVVV
                     SSTQIPFHVKRHLSRALEIPASKIRVIKPRIGGGFGGKQTACVEIFSAIVTLKTGKPA
                     KIIYDRKETFNCSTSRHAMRLKVRLGATNDGIIKAIDIKALSDTGAYGEHASTTIGLV
                     GEKTLPMYNKVEATRFIADVVYTNKMPAGALRGYGASQGCFAVESTINMLANKLKMDP
                     TDLRLKNVVTEGETTFAYGKTLNSVDLIKCINKGKELIGWNEKYPCKHLDNGKIRSVG
                     MAITMQGSGIQGLDKSSAEIKLNDCGDYTLMIGSTDMGTGSDTILAQMAAEVLDTSIE
                     NITVISADTDLVPYDPGSYASSTTYVTGMAVVKAAQDLREKIISAGATKLGVEKDEVE
                     FDGIKLTSKKGSTLLFDIAVDSTIGPDTNQLIGLASHGSPVSPPPFIAGFAETEIDTE
                     TGKVDLVNYVAVVDCGTVINKNLAKIQVEGGIVQGIGMALFEEVRYTEKGSMDTNTFM
                     QYKIPARCDIGNVKVDFVETYEPTGPFGAKSVGEVVINTPLPAIQEAVFNGIGVRINT
                     LPITPEKVFMEMNK"
     gene            5305..5616
                     /locus_tag="PC700_003129"
     CDS             5305..5616
                     /locus_tag="PC700_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582402.1"
                     /translation="MAGNLNNMRAVNNFRGDKNILECLVSFEGRSISQRKVRVFFKEK
                     QNQIEIDFAEEEISKLVENVVLNTSYQEMLYDEIEKQLEIDCIGTWMILSKLKDGSRV
                     H"
BASE COUNT         2172 a          612 c         1090 g         1812 t
ORIGIN      
        1 aaacttgaca aaatcaacct tttaataact agcaaaaaat agcaaaatta tataagcgtt
       61 atttaattat caaaaatgaa agctaaattg catgtcctaa aaaaaatgat tgataacatt
      121 tatttgtgtg ctataattat atagtaaaac aataagtata ttgcataatg tatgcaaatc
      181 gggaggagag acggacatgg aagcatctat acacacaatt gccagcaata atttattgat
      241 aatttttgct attgtaagta taactggaat aatttgtagt aagcttagtg aaataataaa
      301 ggttccagat gtagtgttat atttacttgt aggtatttta ataggaccat catttttaaa
      361 atttatagat attcgtggat ttcaaataga aaatcaacta atacttacat ttggttctgc
      421 atttatttta tatttaggtg gaaaagaaat aagtttaaaa gtattgagaa atgtgaagat
      481 atctgtattc ttattagcta ctttaggagt tgtgatatct gcttttataa tgcaacaagt
      541 aataggattt acttttggga taagtgcaat gactgcttta ctagcaggaa ctataatagc
      601 atcaacagac ccagccactc ttgtacctat atttaatcag gtaaagataa aggatagggt
      661 aaaacaaact gtaataagtg aatcagcttt taatgatgca acaggagcta tactaacatc
      721 agcagtgttg gctgtaattt tgtcaaataa attttcatta ggtgaaaata tttatgaact
      781 ggggacaatg gttattgtcg gagttttagt tggtttaatt acaggaatac tgcttttgaa
      841 attagttaat gataaacctt atggaatatt taaagatttt gcaccaataa tatctgttat
      901 ttcagttgta gttgcatatg aattatctac aaaattaggt ggaagtggat atatgtcttg
      961 ctttattgta ggtataataa ctggtaacaa gaaaaacttt aaaatttggt taagtcaaaa
     1021 atcttatgat gcagattttt atgttgctga aactttaggt actatttgtc gtatggcaat
     1081 atttataata ttgggtagcc aagttaattt ggctcagtta agtaaatatt ttatgccatc
     1141 tgtaattaca gtattagtat taatgtttat agcaagacca ctttgcgttt tagtttgtac
     1201 acttgtagac agggaagcaa aatggagtaa aagtgaaata ttatttatga tgtgggtaag
     1261 ggaaacaggc gtaataccag cagcactttg tggtattatt tcagcaatga aagttcctgg
     1321 atacgaagtg atttcatctg tagtatttat gactatatta attacattag taatacaagg
     1381 aagtactaca aaattagtag ctaaaaaact tggattatta gaagaagaag tgataaatat
     1441 aagtgaaaaa gtatcaactt tttaaagttt tgctttaaaa tagtacctgt taatggctat
     1501 tttaatattg tctaatgatg attattgaaa tagtcatttt tttatgtata agtaatttac
     1561 agaatttaca tattagacat atttattatc aaaatttgtt gaagctatga tataattagt
     1621 tgtatttaaa ataagaatag caaataaggg aggaaattta tgtttacggt tatggatatt
     1681 gtacagccag atacagtaga agaagcgtac tcaattttaa ataaaagaaa aactaaccaa
     1741 gtaattggtg gaagtgcttt tttaagaatg gggaaaaaaa gaataggaac aggaatagaa
     1801 ttatctaaat taaatttaga ttatataaaa gagtatgaag attacgttga gattggttct
     1861 atgactactt ttagaacatt agaaacaagt tccataataa aaaataattt tggaagaata
     1921 atagaagatt cagttaaaaa tataatagga gttcagttta gaaatgtagt aacagttggt
     1981 gcaacagtat tttctaaata tggtttttct gacttgatag tagcattact ttcattagat
     2041 acggaagtag aattatataa tattgggaga attagtcttg aagagtttct aaatagagat
     2101 tatgagaaag atttacttat taagatatac ataaagaaga caaataagaa tgcttcttat
     2161 aaatcactta gaaatgcaaa gagtgattat ccaattctaa atgtatctgt atcaaaacat
     2221 atggaacaat ttaagttatg tgtaggagca agaccgcaga aagctacgat tgcaaaacaa
     2281 gctagtgaat ttctatctaa caatgaaatc aatgaaatta atatagataa agcagtagaa
     2341 atagcatcag aagaactaac ttttggttct aacatgagag catctaggga gtatagaaaa
     2401 gctatgagta aagtactttt aaaaagagct ataatggagg ttatttaatg ttagtaaaga
     2461 ttaatataaa tgggaagaaa aaaagtgttg atatagaacc agaagaatat ctggttgata
     2521 ctttaagaaa agttggtaat ttaagtgtga aaagaggttg tgacacaggc tgttgtggtt
     2581 tatgtacagt tttaattgac aagaaaccta cattatcctg tgcaacactg acattgagag
     2641 ctttagataa agaaataact acaattgaag gtttagaaaa tgaagttaaa gaatttagtg
     2701 aagttttggt aaaagaagga gctgagcaat gtggtttctg ttctcctggc tttatactta
     2761 ctgtacttgc aatgaaggat gagcttgaaa atcctacaga tgatgaaata aagcattatc
     2821 ttacaggaaa tctatgtaga tgtacagggt atatgggaca gttaagagct atcaaaactt
     2881 atctggaggt aaaatagaac atgaatatag ttggaaaagg aatcaagaaa atagatggaa
     2941 tgagtatcac aacaggaaag cctgtatata cagatgattt atcagcaaaa aatgctttgg
     3001 tggtaaagat acttagaagt cctcacgctt atgcaaaaat aaaagatata gatacatcaa
     3061 aggcagaaat ggttgatggt gttgaatgtg ttttaacata taaagatgta ccaaaatctc
     3121 gatttacact tgctggtcaa acatatccag agccatctcc atatgataga ttaattcttg
     3181 aagatgttgt aagatatgta ggtgacgaag tagctatagt ggcagctata gatgaaaaaa
     3241 ctgcaattaa agcaatgaat cttatcaaag taaagtatga agtacttaca cctgttttgg
     3301 attttgaaga ggcaatagga aataaaacta tggtacatga tgaagataga cattgtaact
     3361 ttgatatagg tatgcaaaga gaaaaaaata tagtatccca tcatgattat acaaaaggtg
     3421 atgtagaaaa agtattaagt aattgtgatg tagtgattga agaaacttat tatactcaag
     3481 ctcaagcaca tgcaatgatg gagacgtata gggcatacaa ttatttagac catatgggaa
     3541 gattggttgt agtaagttca actcaaatac catttcatgt aaaaagacat ttatctcgtg
     3601 cactagaaat accagctagc aaaattaggg ttataaaacc tagaattggt ggcggatttg
     3661 gtggtaaaca aactgcgtgt gttgagattt tttctgcaat tgttacttta aaaacaggga
     3721 aaccagctaa aataatttat gatagaaaag aaacttttaa ttgttcaaca agtagacatg
     3781 caatgagatt aaaagttaga ctaggtgcta caaatgatgg cataataaag gcaatagata
     3841 taaaagcact ttctgataca ggagcatatg gagagcatgc ttctacaact attggtttgg
     3901 ttggagaaaa aactttacca atgtataata aagttgaagc aacaagattt attgcagatg
     3961 tggtatatac taataagatg ccagcaggag cacttagagg gtatggagca tcacaaggat
     4021 gttttgctgt tgaatcaact ataaatatgc ttgctaataa attaaaaatg gacccaacag
     4081 atttaaggtt aaaaaatgtg gtaacagaag gtgaaactac atttgcatat ggtaaaactt
     4141 taaatagtgt cgatttaata aaatgtataa ataaaggcaa agaacttatt ggatggaatg
     4201 aaaaatatcc ttgtaaacat cttgataatg gaaaaattag aagtgtgggt atggcaatta
     4261 ctatgcaagg ttctggtata caaggtcttg ataagtcttc agctgaaata aaattgaatg
     4321 actgtggaga ttatactctt atgataggtt ctactgatat gggtacagga agtgatacga
     4381 ttttagctca aatggctgct gaggttcttg atacaagcat tgaaaatata acagttatat
     4441 cagctgatac tgacttagtt ccatatgacc caggttcata tgcttcaagt actacttatg
     4501 taacaggtat ggcagtagtt aaggcagctc aagacttaag agaaaaaata atttctgctg
     4561 gagcaactaa actaggtgtt gagaaggatg aagtagaatt tgatggtata aaattaacta
     4621 gtaaaaaagg tagcacttta ttattcgata tagcagttga ttctacaatt ggtccagata
     4681 ctaatcaatt aataggtctt gcttctcatg gtagcccagt atctcctcca ccttttatag
     4741 caggttttgc agaaactgaa attgacacag aaactggaaa agttgattta gtaaattatg
     4801 tagctgtagt agattgtgga acggttataa ataaaaatct agctaagata caagttgaag
     4861 gtggaattgt tcaagggatt ggaatggcat tatttgaaga agttagatac acagaaaaag
     4921 gaagcatgga cacaaataca tttatgcaat acaagattcc agctagatgt gatattggaa
     4981 atgttaaagt agattttgtt gaaacttatg aaccaactgg accatttggt gctaaatctg
     5041 ttggagaagt tgttataaat acacctttac cagcaataca agaagctgtg tttaatggaa
     5101 ttggtgtaag aataaataca ctccctatta cacctgaaaa agtttttatg gaaatgaata
     5161 aataattatt tatgaaaatt tagactagtt ttacatgtta aacaaccagt tatgtcatac
     5221 ctgttgaaag atatttaata agtagttatt ataatattat gaattttact tataaaatac
     5281 aaaattaaat aaggggatga tgttttggct ggtaatctaa ataatatgag agcagtaaat
     5341 aattttagag gagataagaa cattttagaa tgtttagtca gctttgaggg tcgttcaata
     5401 agtcagagaa aagtaagggt attttttaaa gaaaaacaaa atcaaataga aattgatttt
     5461 gcagaagagg aaatttctaa attggttgaa aatgttgttt taaatacatc atatcaagaa
     5521 atgttatatg atgaaataga gaaacaactg gaaattgatt gtataggtac ttggatgata
     5581 ttatctaaat taaaagatgg tagtagagtt cactgatttt aaaataaatt attagagcct
     5641 aattttaagg ctcttttttg ctgtttttaa ttaacaatag aaaatt
//
LOCUS       DAMJWP010000217         5675 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.224, whole genome shotgun sequence.
ACCESSION   DAMJWP010000217 DAMJWP010000000
VERSION     DAMJWP010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5675
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.224"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            626..1030
                     /locus_tag="PC700_003130"
     CDS             626..1030
                     /locus_tag="PC700_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDF2582403.1"
                     /translation="MIKITIPGRPISKSNFKLHNINGQAWMPSKGKYSKYLAYENMIA
                     GFINQQYQGETVEENLITVLKLFFPNKRMGDLHNYPKSICDGIEKSGIIKNDKQLKPV
                     FLFDFIDKDNPRVEIELYPVSKYDISYNIFEK"
     gene            complement(1287..2282)
                     /locus_tag="PC700_003131"
     CDS             complement(1287..2282)
                     /locus_tag="PC700_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDF2582404.1"
                     /translation="MRNLGNTNMKIKRVGFGGIPIQRITQDDTNLVINELEKQGINFI
                     DSARGYTISEEAIGIAIEGKRNKFFLATKSMSRDYDSMKRDVEISLNNFKTDFIDLYQ
                     FHNVKEEEYDNLFKDKMAYSALLEAKEQGKIKHIGITSHNLNTIEKAIEDGKFDTIQF
                     PYNIVEGQADEVFKKAHEKGIGIIVMKPLAGGALDNATLAIKYILSKDYIDVVIPGME
                     SVEQVRQNVAVLENLVLDEKDNKEIEEIRNSLGKKFCRRCEYCMPCAVGINIPLSFLC
                     EGYYTRYGLKEWAKEKYEVMDVKPTECIDCGLCESRCPYELPIREMLKTVVEKLG"
     gene            complement(2534..3370)
                     /locus_tag="PC700_003132"
     CDS             complement(2534..3370)
                     /locus_tag="PC700_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDF2582405.1"
                     /translation="MIKLGTHLSIAKGFANAAETAVYIGANTFQFFSRNPRGGNAKEF
                     DEKDIIKFQEIRKENNFAPLLAHAPYTMNLGGTKDDVYEFATRVIDEDIRRMDSLGIE
                     YMCFHPGSHIGGGVDFGIDRIVKGLNNAIKGDENITILLETMSGKGTEIGFKFEQLKS
                     IIDKIEHKEKIGVCLDTCHIFSAGYDIVNDLDGVLEEFDKVIGIERLKTVHLNDSMMP
                     FGDKKDRHAPIGEGKIGLKALIDFMEHPSLKHLPFFLETPFDDEGHKRELKMIKEILS
                     SK"
     gene            complement(3858..4421)
                     /locus_tag="PC700_003133"
     CDS             complement(3858..4421)
                     /locus_tag="PC700_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2582406.1"
                     /translation="MKEVLVLRDLECIKAIAHPRRIDILKAFDKSPLSAKQLSQLLEE
                     PHAKINYHIKMLYKVGILELVEEKIKSGIVEKYYYPSAKNVVIGNRILNFSLDNGEEK
                     EELYISKFENMSEVFYKAAEEDVLENENIVDYHDISLTHDELVELSDTMKSKIDEILN
                     KRQHNVEGSKYDIAMVIVPTLEEECPS"
     gene            complement(4519..5448)
                     /gene="glsA"
                     /locus_tag="PC700_003134"
     CDS             complement(4519..5448)
                     /gene="glsA"
                     /locus_tag="PC700_003134"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HDF2582407.1"
                     /translation="MNTLDETLLKEIISSNKKYTNYGQVASYIPELKNARRNDLGICI
                     IDSENNLYSAGNCSTKFTIQSISKPIVLAMALMDNDWEDVFSNVGMEPSGDPFNSIMK
                     LEINDTKKPCNPMINAGAIVTTSLINGSCLEEKEERMLSFFRKLAKNDNIGINYDVYK
                     SEKMTGDRNRAMAYLLKSDGFIRGNVEDVLDLYFKQCSIEIDSVDLARIGINLANYGV
                     DIENGEHLMSEMVSRIVKTFMMTCGMYDASGEFAIKVGIPAKSGVGGGIMASVPGRMG
                     IGVYGPALDKKGNSVAGVKVLEELSNKLKLNIF"
BASE COUNT         1867 a         1029 c          548 g         2231 t
ORIGIN      
        1 aattttttta tctactaacg cttttagata cttaatcttt ttatctattt aattttttta
       61 tctactaacg cttttagata cttaatcttt ttatctattt aattttttta tctactaact
      121 catttagata cttagctttt ttcatttact tatttaactt ttttacctac ttaactcttt
      181 tagatactta gcctttttaa tctattcaac cttttttacc cacttaacct tcttaccttt
      241 taatataaac tctaaaactt caaatatcat tagttatatt ttgacattct tcctatagtt
      301 aatgcaaaaa gattgatttt ttcttataat taatatccca tttacattgc catttattaa
      361 gtctttactt ggcttatatg gatttctttt tactctcttt actaaaccta tacttattaa
      421 tattatgaag gtcttataat agtctaatat gaatatgtcc atcatatatc aaattttaaa
      481 atttgtaagc aaaatactta tcttatatta ctacatctaa agtggaaaat tttacaaaca
      541 agccctgtaa aaacacttta aataacaatt tgcttttaaa tcatatacag attataataa
      601 atactgtata aggatggtga aaaatttgat taaaataaca attccaggta gacctattag
      661 taaatctaat tttaagcttc ataacataaa tggtcaagca tggatgcctt caaagggaaa
      721 atattctaag tatcttgcat acgaaaacat gattgcaggt tttataaacc aacagtatca
      781 aggggaaact gttgaagaaa atctaataac agtcttgaaa ttattctttc caaataagag
      841 aatgggggat ttacataatt atccaaaaag tatttgtgat ggtattgaaa aaagtggaat
      901 aataaaaaat gataaacaac ttaagccagt atttttattt gattttatag ataaagataa
      961 ccctagggtt gaaattgaac tttatccagt atctaaatac gatatatcct ataatatttt
     1021 tgaaaaataa agattttatg tgttattcga cattatattg atactccagt atttgaaact
     1081 atactaatac aacaataata attgtgttat gtctatactc aaacaaatgt ttttaaaaag
     1141 cattaagcaa atatttaata agtattaaac aaatattaaa tttatataat agctataact
     1201 atgtaaattt aatatttgta tataagacca catctaataa aaagaaatcg gagtaaattt
     1261 aatacaccga tttcaataaa taaaatctat cctagctttt caacaacagt cttaagcatt
     1321 tctcttatag gaagttcata tggacatcta ctctcgcaaa gcccacagtc aatacactca
     1381 gtaggtttaa catccataac ctcatatttt tctttagccc actcttttaa tccatatcta
     1441 gtataataac cttcacataa aaaactcaat ggtatattta ttcctacagc acaaggcata
     1501 caatattcac atcttctaca gaatttttta cctaaagaat ttcttatttc ttcaatttcc
     1561 ttattatctt tttcatctag aacaagattt tcaagtacag cgacattttg tctaacctgt
     1621 tcaacactct ccatacctgg tataactaca tctatgtaat ctttagatag tatatattta
     1681 atagccagtg ttgcattgtc taaagcacct ccagctaaag gtttcattac tattatccca
     1741 attccttttt catgagcttt tttaaacact tcatctgctt gtccttcaac tatattgtaa
     1801 gggaattgta tagtgtcaaa ttttccatct tctatagctt tttctatagt atttaaattg
     1861 tgactagtta taccaatatg ttttatctta ccttgctcct ttgcctctaa aagggcacta
     1921 taagccattt tatctttaaa aagattatca tactcctctt ctttaacatt atggaattga
     1981 tataaatcta taaaatcagt tttaaaatta tttaaactta tttcaacatc tctcttcatt
     2041 gaatcataat ctctggacat acttttagta gctaagaaaa atttatttct ttttccttca
     2101 atggcaattc cgattgcttc ctcacttatt gtataccctc ttgcactgtc aataaagttt
     2161 attccttgtt tttctaactc atttataact aagttagtat catcttgagt aattctttgt
     2221 attggaatgc ccccaaagcc aactctcttt attttcatgt tagtattgcc taaatttctc
     2281 atatctttcc ccacctaact tttaataatt ttttatataa tataaatatg ttacctttaa
     2341 ttatacaaca aattatgaga atctttgcaa tatatttaca tactattcat aatataatca
     2401 tactttatac ccagttaatt tgtatacaat ttttgttatt cctatccatt aatttaaatt
     2461 atcacatttc atgattgtat ctccacctta attatgttga gggcggagta tttcacatat
     2521 ttttaataaa ggtttattta ctagatagta tttcttttat cattttaagc tctctcttgt
     2581 gaccttcatc atcaaaagga gtttctaaga aaaatggtaa atgttttaga ctaggatgtt
     2641 ccataaaatc aattaatgct tttaatccta tctttccttc ccctatagga gcatgtctgt
     2701 cttttttgtc tccaaatggc atcatactat catttagatg gacagttttc aatctttcta
     2761 tgcctataac cttatcaaat tcttccaaaa ccccatctaa atcattcact atatcataac
     2821 cggctgaaaa aatatgacat gtatcaagac acacacctat tttttcttta tgctcaattt
     2881 tgtctattat actttttaat tgctcaaatt taaaacctat ttcagttccc tttcccgaca
     2941 tagtttcaag aagtatagtt atattttcat caccctttat ggcattgttc aatcctttta
     3001 ctattctatc tataccaaaa tctacaccac cacctatatg acttccagga tgaaaacaca
     3061 tgtattcaat tcctaatgag tccatccttc ttatatcctc atctatcacc cttgttgcaa
     3121 attcgtatac atcatccttt gtaccaccca gattcatagt atatggtgca tgtgcaagca
     3181 gtggtgcaaa attattttct tttctaattt cttgaaattt aattatatct ttttcatcaa
     3241 attctttagc atttcctcct cttggatttc tactaaaaaa ttgaaatgta tttgcaccaa
     3301 tatatacagc tgtctcagcc gcattagcaa atccttttgc tattgaaaga tgtgttccta
     3361 atttaatcat attaatctcc ttttatttgt actacgactt gtactcaatt ttaattttga
     3421 actactccac cttaactata tttagccaac atatgaagat ggagattcct atttgatagt
     3481 actttttcac taaaaattca taagctctaa gggatgttct atcccaacta atctcattcc
     3541 tcttttaaca tatttctagc attgatttaa tttatattaa tttttaattt taatatttac
     3601 actttaatta tacccatttt aatcaatttc aaatattata atcttacata ctttgtatat
     3661 tatctaagca cttctcagat agtgtataca tcttacacta tacatattat ttacaaatac
     3721 aataggtata agaaaacaaa aaaatagaga ttaaaaatca ttgttttacc cataccaaaa
     3781 actatttttt gattattaag taaattttga tgttcaatct ctaatattat atctttaatt
     3841 aatccataat taattctcta actaggacat tcctcctcaa gagtaggaac tataaccata
     3901 gctatatcat acttagaacc ttcaacattg tgttgtcttt tatttaaaat ttcatctatc
     3961 tttgacttca tagtgtcact aagttcaact aattcatcat gagtaagaga aatatcatga
     4021 taatcaacta tattttcatt ttctaaaaca tcttcctctg ctgccttata aaaaacttca
     4081 ctcatatttt caaattttga tatgtacaat tcttcttttt cttccccatt gtctaaagaa
     4141 aaattaagta tcctattccc tataaccaca ttttttgcac ttggataata gtacttttcc
     4201 acaatccccg actttatttt ttcctcaact aattctaaaa taccaacttt gtataacatt
     4261 tttatatggt agtttatttt tgcatgaggt tcttccaata attgagataa ttgttttgct
     4321 gacaatggcg atttgtcaaa tgcttttaga atatctattc tacgtggatg cgcaatagcc
     4381 tttatgcact ccaaatctcg taataccaat acctctttca ttgacccctc ctaaaaaatt
     4441 tatttaattc atatttcttt acaatttaat tatatattaa attatatgtt gttgtcaaaa
     4501 aaatcctttc ttaatatttt aaaaaatatt taatttgagt ttatttgaca actcctctaa
     4561 aactttaact cctgcgacgc tgttaccttt tttgtcaagc gcaggtccat aaacaccaat
     4621 ccccatgcgt cccggcacag aagccattat accaccccca actccagatt ttgcaggaat
     4681 gccaacttta atagcaaatt ccccagaagc atcatacatt ccacaagtca tcataaatgt
     4741 cttgacaatc ctagaaacca tttcactcat taaatgctca ccattttcta tatctactcc
     4801 ataatttgca agatttattc caattctcgc taaatctaca gaatctatct caattgagca
     4861 ttgtttaaaa taaaggtcta gaacatcttc tacattccct ctgataaatc catcactttt
     4921 caataagtaa gccatagctc tgtttctatc tccagtcatt ttttcagact tatagacatc
     4981 atagtttatt cctatattat cattttttgc taactttcta aagaaactaa gcattctttc
     5041 ttctttttct tctaaacaac tcccatttat aagggaagta gttactatag cccctgcatt
     5101 tatcattgga ttacagggtt tttttgtgtc attaatttca agtttcatta ttgaattaaa
     5161 tgggtcccca cttggctcca ttccaacgtt tgaaaaaacg tcttcccaat cattatccat
     5221 aagtgccata gcaagcacaa ttggttttga aatactttgt atagtaaact ttgtgctaca
     5281 gtttccagca ctataaagat tattttcgct atctatgata caaattccta aatcatttct
     5341 tctagcgttt tttaattctg gtatgtaact agcgacttgc ccataattag tatacttctt
     5401 attagaactt ataatttctt ttaataatgt ctcatctaac gtattcatat gcacctctta
     5461 tattatgtta tatattctat tttagcacaa gtttgtacta taataaaaca gtgttaaaaa
     5521 aaattatact ttaaaactat tttctacaag aatcaattca tttttcaaac aatttaaaca
     5581 gtttaaatag cttaataatt taataatcta gtaatttagt aatctaataa tccagtaatt
     5641 taaattctta taacgtcaaa aatttctaac ctaac
//
LOCUS       DAMJWP010000218         5666 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.225, whole genome shotgun sequence.
ACCESSION   DAMJWP010000218 DAMJWP010000000
VERSION     DAMJWP010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5666
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.225"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            25..876
                     /locus_tag="PC700_003135"
     CDS             25..876
                     /locus_tag="PC700_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HDF2582408.1"
                     /translation="MKNIISAKELISKLEKNDNLVVIDCRFDLINRTYGIDSYKKGHI
                     KGSFILDIDKDLSSPTKEHGGKNPLQDPLILKEKLEKMGVDNDTTIVTYDDGDLNGAC
                     RLFFQLKHLGLKNVYVLDGGITSFVKEGGELEEKVNIPSCTGKEIRPNINNDLVVPME
                     YVKSKLYKKDTVIIDCRANERYQGLVEPAYSKAGHIPSAKNYFCKDLIKSDFENGSLK
                     DIEFLKKFFKDLNNYDEVILSCGSGISACVNSLALRELDIPHKIYIGSFSDWISYDDN
                     EIKTGQE"
     gene            complement(1009..2622)
                     /gene="ggt"
                     /locus_tag="PC700_003136"
     CDS             complement(1009..2622)
                     /gene="ggt"
                     /locus_tag="PC700_003136"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HDF2582409.1"
                     /translation="MKFNAYQHKYSSIRNVVYAKNGAVATSTPLASQAGLEILKKGGN
                     AVDAAVATAATLAVVEPTSNGIGGDAYALVWIEEEKRLYGLNSSGFAPENMELKNYNN
                     MKEMPKYGFGAVTVPGIPAAWSELNKKYGKLSLMECLSPAINYAREGYVVSPNVAKVW
                     KKSYELYEKELIGEEFKPWFDTFSKDGKAPEAGDIFICEEQASTLEEIANTQAESFYR
                     GRLADKIDEYSKKFNGAIRKSDLECFYPTWVEPISTEYKGYKIFEIPPNGHGITVLMA
                     LNILKELELEGNIENVEDIHKIIESLKLAFADSKTYVTDIEHMKVKIQELLSQEYAKK
                     RSLLIDNKEALYPTAGEPYCGGTVYLCTADKDGNMVSYIQSNYINFGSGIVIPRTGIA
                     LHSRGNNFNLDPKHHNVVKPFKKPYHTIIPGFLGKEDKAIGPFGVMGAFMQPQGHIQV
                     LTNMIDFGLNPQEALDAPRWQWIKGKEIEVEPEMPKHIIDSLIEKGHEIKVIHDTVDM
                     GRGQIIFKTEQESYICGTESRCDGHIAVY"
     gene            complement(2847..4784)
                     /locus_tag="PC700_003137"
     CDS             complement(2847..4784)
                     /locus_tag="PC700_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2582410.1"
                     /translation="MTLFRIAMQNVKNSFFNYLMYFISIVFSVFVFFSFKSIEYNEAL
                     SSLGEKTRMSINTSSIVIVVFVFLFIYYSNSFFFNRRRREVGTYSLLGMRKNQIGKIF
                     LYETFLMGIVAILIGIFLGLLFSKLMTMMLVKLMNEMIVVNMNLSIKALVKTLAVFLI
                     IFIVIGIRNTIVIRNKRIIELFNKYPEKYKFKKFIKLKCVLGVLLIAISYLMSISYFI
                     AENIMLSVFILVTIIPGTFLFFSSTMYMIIDAVKKRKSFYYKGQNLIAFSELGFKLKS
                     NSSVLAVIAILIATSVTMLGFTISLYYDIDRNISENYKYSYTINLGNSYVNNEIDKLL
                     YKYKKDNKIIFDKTIELIDKDINIEMYYKNGNSYIEDRTSVDIIRESDFKKLRQYQNN
                     NYEELTSKESVYYVSDSYKKMFFKDFEIEKINLYIKDTNEYDINLFKVQKSILEPEIN
                     SQATFDLIVVKDDVFNQLKLHGNSRLIRLIDIKNEKKELDLTLKFKNIVSENMKFTYP
                     FNFTSSIESYNNLIKLSGLMLFIGIFLSVVFLLCTGSIILFKQLSNIYDDKERYIMLI
                     KLGANNKDIEKIISKQLKIIFLMPLVVGTVHNLFAMSIAQKFIPRSLLVPIIITLVIY
                     FIGYFIYYFLTLKYALNMIKE"
     gene            complement(4771..5538)
                     /locus_tag="PC700_003138"
     CDS             complement(4771..5538)
                     /locus_tag="PC700_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003722224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2582411.1"
                     /translation="MEEILSVENIKKEYGRKGSKHEALKGITFKVYKGEFVGIMGSSG
                     AGKSTLLNIISTMDLPSSGDIYINGKNTIKMKQNELADFRRDNLGFVFQDSNLLDTLT
                     IKENIMLPLSLKNERVSVIENRIKEISKELNIESILDKYPGEVSGGQKQRGAVCRAIA
                     TKPSLVLADEPTGALDSKSARDLLNCLLKLNKDSNKTILMVTHDAISASFCNRILFIK
                     DGIIFTEIVKGESNREFYNKIVNTVSLIGGVNKNDFI"
BASE COUNT         1913 a          910 c          635 g         2208 t
ORIGIN      
        1 atataaacat aggaggtaca ttctttgaaa aacattattt cagcaaaaga acttatatca
       61 aaactagaaa aaaatgataa tctagttgta atagattgca gatttgatct tatcaataga
      121 acttatggta ttgattctta taaaaaagga catatcaaag gcagttttat cttagatata
      181 gacaaggatt tatcatctcc tacaaaagaa catggtggta aaaatccttt acaagaccct
      241 ttaatactaa aagaaaaatt agaaaaaatg ggtgttgata atgacactac gattgtaact
      301 tatgatgatg gagatttaaa tggagcttgc agattatttt tccaattaaa acatttgggg
      361 cttaaaaatg tctatgtact agatggggga ataacatctt tcgtaaagga aggtggagaa
      421 ttggaagaga aagtgaatat accaagttgt acaggtaagg aaattagacc aaatataaac
      481 aatgatttag ttgttccaat ggaatatgtt aagtctaaat tatacaaaaa agatactgta
      541 ataatagatt gccgtgctaa tgagagatac cagggtttag ttgaacctgc ttactcaaaa
      601 gctggtcata taccaagtgc aaaaaattat ttttgtaagg atttaataaa aagtgacttt
      661 gaaaatggct cacttaaaga tatagaattt ttaaaaaaat tctttaaaga tttaaataat
      721 tatgatgaag taattttatc ttgtggttca gggatatctg cttgtgttaa cagtcttgct
      781 cttagagaac tggatatacc tcacaagatt tatattggta gctttagtga ctggattagt
      841 tatgatgaca atgaaatcaa aactggtcaa gaataaggct cataatctat ataaaatcag
      901 catatgatag cttaaatttt gtaaaggcgt cagtcattgt agactttcgc ctttactaat
      961 tatttcatat actctttaaa tttattatat atattttagt tgataatatt aataaactgc
     1021 aatatgacca tcacatcttg attccgtccc acaaatataa ctttcctgtt ctgttttaaa
     1081 tattatttga cctcgtccca tgtcgacagt atcatgaatt acttttattt catgaccctt
     1141 ttcaattaat gaatctatta tatgctttgg catctctggt tcaacttcta tttcctttcc
     1201 ttttatccac tgccatcttg gagcatctag cgcctcttgt ggatttaatc caaaatcaat
     1261 catatttgtt aacacttgaa tatgtccttg tggttgcata aaagctccca ttacaccaaa
     1321 tggacctatt gctttatctt ctttacctaa aaatccaggt attatagtat gataaggttt
     1381 tttaaatggt tttactacat tgtggtgttt agggtctaaa ttaaaattat ttcctctact
     1441 atgtaaagca atcccagttc ttggtatcac aattcctgaa ccaaaattta tgtaattgct
     1501 ctgtatatat gaaaccatat ttccatcctt atctgcagta caaaggtata ctgtaccacc
     1561 acaatatggc tctcctgcag ttggatacaa agcctcttta ttgtcaatta ataaacttct
     1621 ttttttggca tactcttggc ttagtaattc ctgaatcttt accttcatat gctctatatc
     1681 agttacatat gttttagaat ctgcaaaagc taattttaaa ctctctataa ttttatgtat
     1741 gtcttctaca ttttctatat ttccttctaa ttccaattct tttaatatgt taagtgccat
     1801 taaaactgtt attccatgac catttggtgg gatttcaaat attttatatc ccttatattc
     1861 tgtacttata ggttctaccc atgttggata aaaacactct aagtcactct ttcttatagc
     1921 cccattaaat tttttagaat attcatctat tttatcagca agtcttcctc tataaaagct
     1981 ttcagcctga gtatttgcta tctcttctaa tgtacttgcc tgttcttcac atataaatat
     2041 atctccagcc tctggagctt taccatcttt agaaaatgta tcaaaccatg gtttgaactc
     2101 ttctccaata agttcttttt catagagttc atatgatttt ttccatacct tagctacatt
     2161 aggagatact acgtatccct ctcttgcata gtttatcgct ggagataagc attccattaa
     2221 gctaagttta ccatattttt tatttaattc tgaccaagct gcgggtattc caggtacagt
     2281 tactgctcca aacccatatt ttggcatttc tttcatatta ttataatttt ttaattccat
     2341 attttctgga gcaaagccac tagaatttaa accatacaat cgtttttctt cctctatcca
     2401 aacaagggca tatgcatctc ctccaatccc attgcttgta ggttctacta ctgctaatgt
     2461 ggcggctgtt gcaactgctg catcaacagc atttccaccc ttttttagta tttcaagtcc
     2521 agcttgagat gctaatggtg ttgatgttgc aactgctcca tttttagcat acacgacatt
     2581 tcttattgat gagtatttat gttggtaggc attaaacttc ataaatttca gctcctcgtc
     2641 attttttatt tgatattctg cttagattta ttatataaca tcaagtaatg taactgatac
     2701 aaaatgatat aaaatataga ctttttccta actttaattt tatctttaaa taaatatctt
     2761 aaactcaatt ctagtatttt ctatctgcac ttaaataagt tattttattc aaaacatata
     2821 tctaattctt ttgacttaaa aataatttat tcttttatca tgtttagagc atattttaaa
     2881 gttaaaaaat aatatataaa atatcctata aaataaatga ctagagttat gataattggt
     2941 acaagtaatg accttggtat aaacttttgt gctatagaca tagcaaataa attatgaact
     3001 gtacctacaa ctaatggcat taaaaatata atttttaact gttttgatat tattttctca
     3061 atatctttat tatttgctcc aagttttatt aacataatat atctttcctt atcatcatat
     3121 atatttgata attgtttaaa taagattata ctacctgtac ataaaagaaa tactacagac
     3181 aaaaatatgc ctataaacag cattagacca cttaatttta tcaagttatt ataactttca
     3241 atacttgatg taaaattaaa aggataagta aatttcatat tttcacttac tatattttta
     3301 aattttaaag tcaaatccag ttcttttttt tcatttttta tatcaattaa tcttattaat
     3361 ctagaatttc catgtaattt taactgatta aatacgtcat cttttactac aattaaatca
     3421 aacgtagctt gtgaatttat ttctggctcc aagatgcttt tttgtacctt aaacagattt
     3481 atatcatact catttgtatc tttaatatac aagtttattt tttcaatctc aaagtcttta
     3541 aaaaacattt ttttgtatga atcagaaaca tagtacacag attcctttga agttaattct
     3601 tcataattgt tattctgata ttgtcttaat tttttaaaat cactttctct aataatatct
     3661 acagaagttc tatcttctat ataactattt ccatttttat aatacatttc aatatttata
     3721 tctttgtcta taagttctat tgttttatca aagattatct tattatcttt tttatattta
     3781 tataataatt tgtcaatttc gttatttaca taagaatttc ctaaatttat ggtataactg
     3841 tatttataat tttcacttat gttcctatct atatcgtaat aaagtgatat ggtaaaccct
     3901 agcatagtta cactcgtagc tattaatatt gctattacag ccaatacact actattgctc
     3961 tttaatttaa atccaagttc agaaaatgca attaaattct gtcccttata ataaaaactt
     4021 tttctcttct taacagcatc aataatcata tacatagttg atgaaaaaaa taaaaaagtt
     4081 ccaggtatta ttgtaactaa tataaaaact gaaagcataa tattttcagc aataaaataa
     4141 ctaattgaca ttaaataaga aatagctatt aataaaacac caagtacaca ttttaacttt
     4201 ataaattttt taaacttata cttttctgga tatttattaa ataactctat aattctctta
     4261 tttctgataa ctatagtatt tcttattcca ataactataa atattattaa aaataccgct
     4321 agtgtcttaa ctaaggcctt tatacttaag ttcatattta ctactatcat ttcattcatt
     4381 aattttacaa gcatcatagt cataagcttt gaaaataaca gtcctaaaaa tatacctatt
     4441 agtattgcaa ctatacccat caaaaaagtt tcatataaaa atatttttcc tatctgattt
     4501 ttcctcattc caagtaaact ataagttcct acttctcgcc tacgtctatt gaaaaaaaat
     4561 gagtttgaat aatatataaa taaaaataca aataccacta ttacaattga acttgtatta
     4621 atactcatcc tagttttttc tccaagtgaa ctcaacgctt cattatactc tatagattta
     4681 aaactaaaaa atacaaatac actaaataca attgaaataa agtacattaa ataattaaaa
     4741 aaactattct ttacattttg cattgcaatt ctaaataaag tcatttttat taactcctcc
     4801 aattaaggaa actgtattta ctattttatt atagaactct ctgttacttt ctccctttac
     4861 aatttctgtg aatattattc catcctttat aaagagtatt ctattgcaaa aactcgcact
     4921 tatagcatca tgagttacca tcaatattgt tttattgctg tctttgttta attttaatag
     4981 acaattcaac aaatccctag cagatttaga atctaatgct cctgtaggct catctgcaag
     5041 tactaatgat ggttttgttg ctatagctct acatacagct cctctttgct tttgtccacc
     5101 agaaacttcg ccaggatatt tatctaatat agattctata tttaattctt ttgaaatctc
     5161 ttttattcta ttttctatta ctgatactct ttcatttttt aaagaaagag gcaacattat
     5221 attttctttt attgtaagtg tatccaaaag attagaatct tgaaatacaa agcctagatt
     5281 atcacgtcta aaatctgcca attcattttg cttcatttta atagtattct ttccatttat
     5341 ataaatatct cctgatgaag gtaaatccat tgtagaaata atatttaaaa gtgttgattt
     5401 accagcaccg gaagacccca taataccaac aaattctcct ttataaacct tgaaagttat
     5461 tccttttaga gcttcatgtt ttgaaccttt tcttccatac tcctttttta tattttcaac
     5521 agataatatt tcttccatag ttaataatca cctttcatca caattagtta tttatcaatt
     5581 ctcacaaata tattatatat attaaaatgg cttttaaaca tttaattata ttaatttaag
     5641 gttacatttt cgtaagtatt aattat
//
LOCUS       DAMJWP010000219         5629 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.226, whole genome shotgun sequence.
ACCESSION   DAMJWP010000219 DAMJWP010000000
VERSION     DAMJWP010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5629
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.226"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            502..1911
                     /locus_tag="PC700_003139"
     CDS             502..1911
                     /locus_tag="PC700_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HDF2582412.1"
                     /translation="MIFSSLIFLFLFFPVTLICYYLANPKYKNVMLLLASIAFYAWGE
                     PKYIFLMILSIIVNYIFGIKVSGSKNKKMWVVLSVIYNISILAVFKYSNFFIDNINFL
                     FNTDITIPEVKLPLGISFFTFQIMSYVIDVYRNDAKVQRKLANLALYISLFPQLVAGP
                     IVRYQTVADSIEYREHNWDKFTQGVNRFVVGLGKKVILANQLGVIADGVFNKSAGDLS
                     RLEVWLGIVCYTLQIFYDFSGYSDMAIGLGKMFGFEFLENFNYPYISQTVSEFWRRWH
                     ISLGTWFKDYVYFPLGGSRVSKAKLYRNLFVVWFLTGMWHGASWTFIIWGLYYGILIS
                     VEKAFLEKFLYKMPRIVRHIYLLLIVMIGWVFFRVDDLPAAIKFIGIMFGAGNYPVIS
                     DAFNVFIIEYWYIIVGAILLSTPIVKVVKSLLIKKNKNTLNSTLYQVTNVVFIVSCMF
                     IVIIMLSSSTYNPFLYFRF"
     gene            1927..3129
                     /locus_tag="PC700_003140"
     CDS             1927..3129
                     /locus_tag="PC700_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582413.1"
                     /translation="MSKKKPRIVAISFLIIVFGFFILNFTLPKKKLSITENRNLAKFP
                     TVENLKKCDFIAKFEEYFNDHFAFRDEMISINTRTQANLNKTKVGNYYLGKDNWILGM
                     FPEILDQKQLSRYEKSINYLSNLSKDMGKNVYFTMMPHKTNMLKHLYPKYVDNMDNID
                     INLKNFKNRLNPDLIKYIDMDEYFLSNYSDKERENLYFKTDHHWTGYGAFEGFKMMAS
                     NMDLGLSKQDLDKHFSSYKKSIITDKKFIGSYNQNIDMLIKENEYVVYMTKDNQDYSF
                     DINGEEKEEKEEKEVYATERDNKEWDYGGAYIRGAKTNILTIKNPKALVDKKILIFRD
                     SYQAPTTLLFADMFKEVEIADPRNINSIYKTYKEIIEESNADIVMFMYNSSGFDSMID
                     SMIDKGIK"
     gene            3330..4313
                     /locus_tag="PC700_003141"
     CDS             3330..4313
                     /locus_tag="PC700_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582414.1"
                     /translation="MKEVFNVHIMQSQNKKIKQKYYLTKDGWLLGNFAKLLSDYHIKS
                     YSKAINNLSKLNRKKDFYFVSLPHKTNMLKHLYPSGIEGKNNIDINKENLEKQLTDIN
                     FLDIDDYFYRSFTEKEREKFFLKTDHHGNSLGAFEEYKFILDKLDLGLSKSEIDSHLK
                     RYSTRYVNDKVFIGSYNKKMGYPLKKKESISYVEISDANYKYFISNGTKNTEINENII
                     KAKLKDKREWDYGGAYMRGCDCHILKIRNSSSLTNKKALIFRDSYQAPMTWMLADTFK
                     ELEILDPRYIKNIDMTYDEIIKNSNANVVLFMYNSFGFAGMIQEMIDKGIA"
     gene            complement(4763..5206)
                     /locus_tag="PC700_003142"
     CDS             complement(4763..5206)
                     /locus_tag="PC700_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582415.1"
                     /translation="MKKNMLYLGIVYFICGILLVLLATFTEFSFEAFIWGLSGAALGP
                     GICMIFQYMYWSKPERAVDYEEKIKNQRIEMNDERRVMLRDKAGRITNQIMSYVLVIL
                     IFIVSILSVFSVMVISKWVLVVLGLLILFQFICSVVVYNKLDKKL"
     gene            complement(5365..>5629)
                     /locus_tag="PC700_003143"
     CDS             complement(5365..>5629)
                     /locus_tag="PC700_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2582416.1"
                     /translation="ISDVSWSEFIHQLKYKANWYGRQIVKVGKFFASSQICNKCGYKN
                     EEVKDLNIREWICPSCNETHDRDINASINILKEGLRLITIQNK"
BASE COUNT         2196 a          699 c          775 g         1959 t
ORIGIN      
        1 caatttcaac aaatagtaac ttttttacaa caaatggaaa ctatggaagc aataattaaa
       61 ataaaattta taatcaatca taattattta gtataatgat tcgatattat tttaaagtat
      121 ttgtcattta aaacaaactt gcgataaaag catatctgaa agtattctga ttctaatact
      181 taataaagag ttctttttac agttatagat ataaacaaca tctatcttaa tagactgtat
      241 agaggcacaa tcaaaggtaa acacatatag ttaagtaata tgtccttgat ttttctttat
      301 tataatagct ttattaattc attttaatat tacatcattt taaaaaaatt atatgaaaag
      361 tcaaaattta catatagatt aattaaactt gtaaaaacac atataaattc atacaattaa
      421 gtacttaaaa cttgaaaata cttactttgc tatatatttt agataaagat ataattgatt
      481 tggttaagaa aggagtctaa tatgatattc agtagtttaa tatttttatt tttattcttt
      541 ccagtcacac tcatttgcta ttacttggca aatcctaagt ataaaaatgt tatgttactt
      601 ctagcaagta ttgcattcta cgcatgggga gaaccaaaat atattttttt gatgatacta
      661 tcaataatag taaactatat atttggtatt aaagtttctg gcagtaaaaa taaaaaaatg
      721 tgggttgtgc tttctgttat ttacaatata tctattcttg ctgtatttaa gtatagcaat
      781 ttttttatag ataatattaa ttttttattc aatacagata taactatacc tgaggttaaa
      841 ttacctttag gtatctcatt tttcacattc caaataatga gctatgtaat agatgtatat
      901 agaaatgatg ctaaagttca aagaaaatta gctaatttag cattatacat aagtttattc
      961 cctcagcttg tagcaggtcc tattgttaga tatcagactg ttgcagatag cattgagtat
     1021 agagaacaca attgggacaa atttactcaa ggtgtaaaca gatttgttgt tggtcttgga
     1081 aaaaaagtaa ttctagcaaa tcaattaggt gtaatcgcag atggtgtatt taataagtca
     1141 gctggagatt tatcaagact tgaggtatgg ctaggtatag tttgctatac acttcaaata
     1201 ttctatgact ttagtggcta tagtgatatg gccattggtt taggtaagat gtttggattt
     1261 gagtttttag aaaatttcaa ctatccatat atctctcaaa cagtatctga gttttggaga
     1321 agatggcata tatctttagg tacttggttt aaagactatg tgtacttccc attaggtgga
     1381 agtagagtct ctaaggcaaa actatataga aatctatttg tagtatggtt tttaaccggg
     1441 atgtggcatg gagctagttg gacttttatt atatggggtc tatattatgg aatattaata
     1501 tcagtagaaa aagcattctt agaaaaattt ctatataaga tgccaagaat tgtaagacac
     1561 atatatctac ttcttatagt catgattgga tgggtatttt ttagagttga tgatttacca
     1621 gcagctatta aatttatagg tattatgttt ggtgctggaa attatccggt tatcagtgat
     1681 gcattcaatg tatttataat tgaatattgg tatataattg ttggggcaat ccttctatca
     1741 actcctattg taaaagttgt aaaatcatta ttaattaaga aaaataaaaa tacattaaat
     1801 agcacattat atcaagttac taatgtggtt ttcattgttt cttgtatgtt tattgtaata
     1861 ataatgttaa gtagctcaac ctataatcca ttcttatatt ttagattcta attgggaggt
     1921 ataaacatga gtaaaaaaaa accaaggatt gttgccattt catttttaat tatagtattt
     1981 ggatttttca tactaaactt cacacttcca aagaaaaaat taagtataac tgaaaataga
     2041 aatcttgcaa aatttccgac tgtagaaaat ttaaaaaaat gtgactttat agccaagttt
     2101 gaagaatatt ttaatgacca ttttgcattt agagatgaaa tgatatcaat aaatacaagg
     2161 actcaggcta atttaaataa aactaaggta ggtaactatt atctaggaaa agataactgg
     2221 atattaggta tgtttccaga aattttagac caaaaacaat taagtcgata cgaaaaatcc
     2281 attaattatt tatctaatct aagtaaagat atgggaaaaa atgtgtattt tacaatgatg
     2341 ccacataaaa ctaatatgtt aaaacatttg tatcctaaat atgttgataa tatggataat
     2401 atagacataa atttaaagaa ttttaaaaat agattaaatc cagatttaat taaatatata
     2461 gatatggatg agtatttttt aagtaactat tcagataaag aaagagaaaa tttatatttt
     2521 aaaactgacc atcattggac tggatatgga gcatttgaag gctttaaaat gatggcatca
     2581 aatatggact taggtctatc taagcaagat ttagacaagc attttagtag ctataaaaag
     2641 tctataatta ctgataagaa atttatagga agttataatc aaaatataga tatgctaatt
     2701 aaagaaaatg aatatgtagt ttatatgaca aaagataacc aagattatag ctttgatata
     2761 aatggagaag aaaaagaaga aaaagaagaa aaagaagttt atgcaactga aagagataat
     2821 aaagagtggg attatggtgg tgcatacatt agaggagcta aaactaatat attaactata
     2881 aaaaacccta aagcattagt agataaaaaa atactaatat ttagagattc atatcaggct
     2941 cctacaacac ttttatttgc tgatatgttt aaagaagtag agatagcaga ccctagaaat
     3001 ataaactcaa tatataaaac atacaaagag ataatcgagg aaagtaatgc agatattgta
     3061 atgtttatgt ataatagttc tgggtttgat tcaatgatag attcaatgat agataaagga
     3121 ataaagtagt aaataaaaag cctctattaa attcaaaatt taatagaggc tttttattgt
     3181 ttatttttta ttgctttggt aaaaataaac taatcaaaat aaaagtaagg agatgtagca
     3241 tattgaacaa aagacatagt ctacctatag tatcagtgat attattgatg gtcttaatta
     3301 tctttaataa taaccaaaat tatattacaa tgaaagaagt ttttaatgtc catattatgc
     3361 agagtcaaaa taaaaagatt aaacaaaagt attatctaac taaagatgga tggctattag
     3421 gcaactttgc taaattatta tctgattatc atataaaatc ttactccaaa gccattaata
     3481 acttgagtaa gttaaataga aaaaaagatt tttactttgt ttcacttcct cataagacaa
     3541 atatgcttaa gcacttgtat ccaagtggta tagaaggtaa aaataatata gatattaaca
     3601 aagaaaactt agaaaagcag ttaacagaca taaatttctt agatatagat gattactttt
     3661 atagaagttt taccgaaaaa gagagagaaa aattcttttt aaaaacagac catcacggaa
     3721 attcattagg agcatttgaa gaatataagt ttattcttga taaattagac cttggcttat
     3781 ctaaatcaga aatagatagt catttaaaaa gatacagtac tcgatatgta aatgacaagg
     3841 tctttatagg tagttataat aaaaagatgg gatatccact taagaaaaaa gagtctataa
     3901 gctatgttga aataagtgat gcaaattaca aatattttat tagtaatggt actaaaaata
     3961 ctgagattaa tgaaaatatc ataaaagcaa aattaaaaga taaaagagaa tgggactatg
     4021 gtggagctta tatgagaggt tgtgattgtc acatacttaa gattagaaat tcaagctctt
     4081 taactaataa aaaggccctt atatttagag attcgtacca agcacctatg acttggatgt
     4141 tggctgatac ttttaaggaa ctcgaaatac tagatcctag atatataaaa aatatagata
     4201 tgacctatga tgagataatt aaaaacagta atgcgaatgt agttttattt atgtataata
     4261 gctttggatt tgctggaatg atacaagaaa tgattgacaa gggaatagca taacaatttg
     4321 aatcagctcc tttttataca aaaaatagtt ctcaataatt cctaatctat atgataattt
     4381 agtacatctc tttaacaata aaaataaaat cacaaaaatt cttataccat cacttatctt
     4441 tatgataaat tggttgagaa aaaattttat aatttatatg atatgcaatc ttaattaatt
     4501 ctttatctac tgcaaccttt aataaaagaa accatccaag actattaata tgaatttata
     4561 taaaaattac tttcaaataa agtactgcag tatttttatt taaagtatct ataatcttta
     4621 accacaaaaa gccacaaaat acaatgtata cttatggctt tttataaatt tatataattg
     4681 tatataaaga tatattctaa aactgtctta agaatctaaa gtttaaaaat ccaatatttt
     4741 tattgtgaat ttaaactttt tattacaact ttttatctaa tttattgtat acaacaacac
     4801 tacaaataaa ttgaaataaa ataagcaatc caagaacaac taaaacccac ttgcttatta
     4861 ccattacaga aaatacactc aatatagaaa caataaatat aagaattaca agaacatagg
     4921 acatgatttg attagtgata cgtccagctt tatcacgtaa cataactctt ctttcatcat
     4981 tcatctctat cctttggttt tttatctttt cttcataatc tactgctctt tcaggcttac
     5041 tccaatacat atattgaaaa atcatacata tacctggacc taaagcagct ccagataatc
     5101 cccaaataaa agcctcaaat gaaaactcag taaacgtagc caataaaaca agtaaaattc
     5161 cacaaataaa atatactatt ccaagatata acatattctt tttcattttt actcctcctc
     5221 ataaataaat atttcttcaa tactcatctg aacctcccat gactaaagtc acagggttcc
     5281 tgcttcacag aattttgcat actaaaatat gtcttactaa ttctccacag gccatccccg
     5341 tagttcctac ggttcttaca tacgttattt attttgaatt gttattaatc ttaatccttc
     5401 ttttaatata tttatacttg catttatatc tctatcatga gtttcattac aactaggaca
     5461 aatccattct cttatattta agtccttaac ttcctcgttt ttatacccac atttattgca
     5521 tatttgtgaa cttgcaaaga atttgcctac ttttacaatt tgtctaccat accaattagc
     5581 cttgtatttt aattgatgaa taaattctga ccatgataca tcagaaatt
//
LOCUS       DAMJWP010000220         5608 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.227, whole genome shotgun sequence.
ACCESSION   DAMJWP010000220 DAMJWP010000000
VERSION     DAMJWP010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5608
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.227"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            240..1067
                     /locus_tag="PC700_003144"
     CDS             240..1067
                     /locus_tag="PC700_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HDF2582417.1"
                     /translation="MDLSKKVKILENASDGKSEFEILEFEKLEGASSPLMAMGLYFAR
                     EAGLKARQIKIKLNNSSIKTEAGALYYYKGNISSETKIGGVGGLFKKGISGALTNESM
                     MKPVYKGTGEVYLEPSFRHYLFMSLDNDSIIVDKGLFYCCSDTIEVKAFTQKNFSSAL
                     LGGEGFFQIELSGTGVIVLECVVPQSEIVEYELKNGEELKVDGNFAIARTSGVNFSVT
                     KSDKSLIGSALNGEGFLNTFTGNGTVWLAPTQVMYEKLSFGAMPTNDNMNNKVSKQK"
     gene            2084..2548
                     /locus_tag="PC700_003145"
     CDS             2084..2548
                     /locus_tag="PC700_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2582418.1"
                     /translation="MNLNKVKFTEEHAIEVTNWKYEGKYSIYNLPPWEEIKKKNFSLS
                     KEDKRKNFISFINDNKELIGFINLLDEGSYVFFGIGIKPDYCGMGIGKEIISLGLQEC
                     RNKYSTKPIVLNVRTWNMRAVKCYKSQGFKIVETQVQKTHLGDGEFFVMRYQ"
     gene            complement(2815..3459)
                     /locus_tag="PC700_003146"
     CDS             complement(2815..3459)
                     /locus_tag="PC700_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2582419.1"
                     /translation="MFGQSFQNCLVLIKENFKLSTLTNLICSIILLCMIPLIQGVANL
                     DRISTAICLENYVAIIGIVMLVPVFSLEQSKEIDEIVSSKSLSQVIVQLIRILMSIIG
                     IVVLIIGFVNVLIYLGCNFPVVEYIIGSFASAMFIGSLGMFFSNLFNNTIIGYMISIG
                     YLILNMMTKGKYVGKFFIMSMSQGSFDEKYWLILGSVVLIIASLLIKPIKRKIR"
     gene            complement(3389..4678)
                     /locus_tag="PC700_003147"
     CDS             complement(3389..4678)
                     /locus_tag="PC700_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2582420.1"
                     /translation="MNLFIKECKNILKSMVYYIFIVVLVFFYISQLGSSVSNDVSKNI
                     PNDGNPLVAPQKVENIDEYYSVHNQYPYGQKESMKTEKVIPNATINLIREYQKNTYTT
                     YPTGVVKYIKLDQNKTKTIEKYIEEITGKSISEINLIWKTDESIKIDYSKVSLERFEE
                     IMQRVDDILGGGSAYAFDKLQRFGVVPVTYDEAMKNYNYTIQKDKVSNGYARIFCDYI
                     GIVLSIFPVFVAVFMSLKDKRSRMREIIYSKQVSSCKIVLSRYFALVFIMILPVLILG
                     MKDLLPLIFFAKDKGISIDNLAFIKYTLWWILPTLMIVTAVGMSITILTDTPVAIIVQ
                     LIWWLYSINSTGLKGDYSTMGLMIRHNSVLNGDIIIQNFNQIMINRIVISLGALVLVL
                     LAIFLYEQKRRGNLDVWAKFSKLFSSNKRKLQIKHFN"
     gene            complement(4675..5547)
                     /locus_tag="PC700_003148"
     CDS             complement(4675..5547)
                     /locus_tag="PC700_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2582421.1"
                     /translation="MEMKIAVSNLSKRFGKKQALDNINLEIDSGMFGLLGRNGAGKTT
                     FMRILATLLSKTEGSIHMCGVPIENTKEIRNMVGYLPQDFSMYPNMTVYKAMDYLAVL
                     SDIPLRKRRKIIAKLLSKVNLTNYQNVKVKSLSGGMNRRLGIAQALIHNPRVLIVDEP
                     TAGLDPEERIRFRNLLCEIANDRIVILSTHIVGDIEATCENVAILDSGKVIYNGSTEA
                     LAKLAEGKVYSIEVDKKDIENIKSRFIVIGMLTHGGKAILRIISDDKPFETAVNCNPT
                     IEDGYMLIMGGDNI"
BASE COUNT         2035 a          775 c          698 g         2100 t
ORIGIN      
        1 ataattttaa catgcccata aaatataata cccacaaatg ataaataata ctcaaaatat
       61 ctattgaaat aactatacat ccttaaataa cttaacttaa atctaaacat ttcaaaaact
      121 tcttaaaaat atgtaaaaat ttgatgataa agtagcgatt ttttcaaaaa aaatgtaata
      181 tagagttata tcttataata ttttagagat aaattttatc atagttggga gaaatttgta
      241 tggatttaag caaaaaagta aaaattttag aaaatgcatc agatggcaaa agcgaatttg
      301 aaatattaga atttgaaaaa cttgagggag cttctagccc attaatggca atgggattat
      361 attttgctag agaagctggt ttgaaagcta gacaaattaa gataaaatta aataattcat
      421 caataaaaac agaagctggt gcattatact attataaagg aaatatatct tctgaaacta
      481 agattggtgg tgttggaggt ctttttaaaa aaggaatatc aggagcactt actaatgaat
      541 caatgatgaa acctgtatac aaaggtactg gagaagttta tttggaacct tcttttagac
      601 attatttatt tatgagtctt gataatgatt ctataattgt tgataaagga ttattctatt
      661 gttgctctga tacaatagaa gtaaaagcat ttacacaaaa gaacttctca tctgctttac
      721 ttggtggtga aggcttcttc caaatagaat taagtggtac tggtgtaatc gttctggaat
      781 gtgttgtacc tcaaagtgaa attgtagagt atgaattgaa aaatggagaa gaattaaagg
      841 tagatggtaa ctttgcaata gcaagaacat ctggggttaa tttttctgtt acaaaatcag
      901 ataaatcttt aattggttct gctttaaatg gtgaaggttt cttaaataca tttactggta
      961 atggtacagt ttggctagct ccaactcaag taatgtatga aaaactaagt tttggagcaa
     1021 tgcctactaa tgataatatg aataataaag tttctaagca aaaatagttg ttttataaat
     1081 atttattaaa tcattattga ttttataaag tcactataaa ttttattaga ttattattat
     1141 aaaatcagag tgtggaaatg tttatgtgta ttctttaaat aaaaatttcc acattctgta
     1201 aggttattat acttgatata taaaattcaa tttaggaaca tagtctaaca tttataaaca
     1261 tattaaggta atgatttctc ttggtaatta ttctcatatc ttattctttg attatattta
     1321 tcttattcct agcccttatg tcatattata tttcttatct tattatttat aattagcatt
     1381 aacttatcta aaatcttgta tttttttaat agtaaagttt acatctatat taaaaagttt
     1441 acatctatat taatttcata aaacaatcaa ggtagtcaag agattcaact ttatttgatt
     1501 ttaaaaattt aagtgaattt gttcttatac acatcttttt aaaattatat ctgtagaaaa
     1561 tatcatttag agctataatt aaattattaa attttagaaa atttattaaa aaatataagt
     1621 taaattaata tttttaaaga ttctttttta taactactag aatactaatt tagtagttat
     1681 tgtatctcta aagtttaagc atggatagat atacatttga taatttgcct ataatttacc
     1741 tactacttgg agtattggag caaatacaat atattaataa taagttccta aaaccaataa
     1801 attaactaat acacataaag atattgatgt tgattataca actataagaa acagatttaa
     1861 cagaatttaa tcaagctaag aaattacata tatcgtgatg tttttgaatt agaaggtaat
     1921 tcaaaattag tttatagtct tctgtgaatc caataaaaag tacagggtac aagaattgtt
     1981 agtaaaattt ttaaagattt tatggataga tataaatagt tttacttatt ttgtaaaatt
     2041 taaaatacca ttaattacct taaatatttt aataggagga catatgaatt taaataaagt
     2101 taaatttact gaagaacatg ctattgaagt tactaattgg aaatatgaag gtaaatattc
     2161 aatatacaac ctacctccat gggaggaaat taaaaagaag aatttttcac tatcaaaaga
     2221 agataaaaga aaaaatttta taagttttat aaatgacaat aaagaattga taggttttat
     2281 aaacttattg gatgaaggaa gttatgtatt ctttggtatt ggtataaaac cagattattg
     2341 tggaatggga attggcaaag aaataattag cctaggatta caagaatgta gaaataaata
     2401 tagtactaaa ccaattgtat taaatgtaag aacctggaac atgagagctg taaaatgtta
     2461 taagtctcaa ggatttaaaa tagttgagac tcaggtacag aaaacgcatt taggggatgg
     2521 agaatttttt gttatgaggt atcaatagtt tataacacta gtcatattgc taatatgaca
     2581 attaaaatga aaaaatacat gtgtgtatgt catagcaata agtgtctttg aaatataaat
     2641 ttgagagcct ttgataaaat tcggtataca tatatatttt atactacaga tatataaatc
     2701 tatctacata cataaagaga ttgtaagtag gtttgtgtat ataaaaataa atttatattt
     2761 cataaatgta ctttttattt gtattacctt tattttatct aagctctcat tttattacct
     2821 aatcttcctt tttattggtt ttattaagag agatgcaatt atcaacacaa cacttccaag
     2881 tatcagccaa tatttctcat caaaactacc ttgactcata gacattataa aaaattttcc
     2941 aacatacttg cctttagtca tcatatttaa aattaaatat cctatagata tcatgtaacc
     3001 tatgattgta ttattaaata aatttgagaa aaacattcct aaacttccta taaacatagc
     3061 tgatgcgaaa cttccaataa tatactcaac tactggaaaa ttacatccaa gatatattaa
     3121 aacatttaca aaacctatga ttaataccac tatacctata attgacatca gtatcctaat
     3181 taattgtaca ataacttgag ataatgattt tgaagatact atctcatcaa tttcttttga
     3241 ttgctctagc gaaaatacag gtaccaacat tactatccct attattgcaa cataattttc
     3301 taaacatata gctgttgaaa ttctgtctaa attagctact ccttgtatta gaggaatcat
     3361 acatagcaat ataattgaac atattaaatt agttaaagtg cttaatttga agttttcttt
     3421 tattagaact aaacagtttt gaaaactttg cccaaacatc aagattcccc cttctttttt
     3481 gctcatataa gaatatagct aacaaaacaa gcaccaatgc tcccaagctt atcacaattc
     3541 tattaatcat aatttgattg aagttttgta taataatatc cccatttaat acactattat
     3601 gtcttatcat tagccccatt gttgaataat cacctttgag tcctgtagaa tttatactgt
     3661 aaagccacca aattaattgc acaataattg ctactggtgt atctgttaat atagtaatac
     3721 tcattcctac agctgtaact atcataagag ttggtagtat ccaccataat gtatatttta
     3781 taaatgctaa gttatcaata gatatacctt tatctttagc aaagaaaatc aaaggaagta
     3841 aatctttcat acctaaaatt aatacaggca atatcattat aaagacaagt gcaaaatatc
     3901 ttgataaaac tattttgcat gaagatactt gttttgaata aataatctct ctcattcttg
     3961 aacgcttatc tttaagtgac ataaaaactg ccacaaatac tggaaatata cttaaaacta
     4021 tacctatata atcgcaaaaa attcttgcat acccattact aactttatct ttttgtattg
     4081 tatagttgta attcttcata gcttcatcat aggtaactgg aactactcca aatctttgta
     4141 acttgtcaaa agcatatgca gaccctcctc ctaaaatgtc gtctactctt tgcattattt
     4201 cttcaaatct ttcaagagat actttagaat aatctatttt tatactttca tcagttttcc
     4261 aaattaaatt tatttcgcta atacttttac cagttatttc ttctatatat ttttctatgg
     4321 tcttcgtttt attttggtct aattttatat acttaactac ccctgttgga tacgttgtat
     4381 atgtgttttt ttgatattct ctaattaggt ttatagttgc atttggaatg accttttctg
     4441 ttttcataga ttctttttga ccatatggat attgattgtg tacagagtaa tattcgtcaa
     4501 tattctctac tttttgtgga gcaactaatg gatttccatc attaggaata tttttactta
     4561 catcatttga aactgaactt ccaagttgag aaatatagaa aaaaactaaa actacaataa
     4621 aaatataata taccatactc tttaatatat ttttacattc ttttataaat agattcatat
     4681 attatctcct cccattataa gcatatagcc atcttcaata gttggattac aatttactgc
     4741 tgtctcaaaa ggtttatcat ctgatataat cctaagtata gcctttccac catgagtcaa
     4801 cattcctata actataaatc tactttttat attttcaatg tcttttttat ctacttcaat
     4861 actatatact ttcccttcag ctagttttgc taatgcttct gttgagccat tgtatatgac
     4921 ttttccacta tccaatatag ctacgttttc acatgtagct tctatatctc caacaatatg
     4981 tgtagataaa ataacgattc tatcatttgc aatttcgcat agtaaatttc taaatcttat
     5041 acgttcttct gggtcaagac ctgctgtcgg ctcatccact attaaaactc tgggattatg
     5101 aattaatgct tgtgctatcc caagccttct attcatacct ccagataaag attttacttt
     5161 cacattttga taattagtaa ggtttacttt gcttaataac tttgctataa tttttcttct
     5221 ttttcttagt ggtatatccg ataaaactgc taaataatcc attgctttat atactgtcat
     5281 gttaggatac attgaaaaat cttgtggcaa ataaccaacc atatttctaa tttccttagt
     5341 attctcaata ggaactccac acatatgtat tgaaccttct gtttttgata ataaagtggc
     5401 taaaattctc ataaatgtgg tttttcctgc tccatttctt cctaaaagac caaacatacc
     5461 actgtcgatt tcaagattta tattatcaag agcttgtttt ttcccaaatc ttttactaag
     5521 attggatact gctattttca tttccatata taaatacacc cccagaaata attttataga
     5581 attttatttt tataaatttt tactgtat
//
LOCUS       DAMJWP010000221         5603 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.228, whole genome shotgun sequence.
ACCESSION   DAMJWP010000221 DAMJWP010000000
VERSION     DAMJWP010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5603
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.228"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            132..632
                     /locus_tag="PC700_003149"
     CDS             132..632
                     /locus_tag="PC700_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDF2582422.1"
                     /translation="MRKNSINIALTAAITAIYAVLTLSLGFISYGPIQFRVAEIMMLL
                     AFLDKGYIVGLTLGCFLANVIGPYGVPDIISGTLATFISASMIYITRKLGKQNKSTLV
                     IASLWPTVINSIVVGLMLNVFFGLPLILSMIQVGFGEFVVVTIVGVPFFCFLMKKYKN
                     VINIKF"
     gene            689..1675
                     /locus_tag="PC700_003150"
     CDS             689..1675
                     /locus_tag="PC700_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDF2582423.1"
                     /translation="MSKVKRLTDVWIYINDKQKFTIKELSEEFNLSAKTIQRYLKELN
                     KMGLPIQAEQGRNGGYRVLNNSYIPPVIFTEKEVMIILFALKSLQIYNYKLMEIEINS
                     IMRKVSYERKSSIKVGIENIKRYIGFVANDTNDGDFKSSNVTEFFRASSESLILNIKY
                     KCGNQILEKNVCPIGIYLNKGAWFSPAYDCKSDKIILVHIDNIIDIKRTGESKKIHIN
                     LEEWLHEVYNNLYGNSSTTKKFGEIIYLNVLLTKEGVSKIKDSSWNLDMELNEDGSGI
                     ISTFIKVEDIDCYASILFFIGNNAKVIEPKFLNKLLYDKANELRYFYEENMV"
     gene            1725..2447
                     /locus_tag="PC700_003151"
     CDS             1725..2447
                     /locus_tag="PC700_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HDF2582424.1"
                     /translation="MENFNKNAKIIGIGVEGINIINEVEEKIKANMDIEKININQEIE
                     KEYVRSLLDGVDILFLIYSSEDKHIRDIIKAVSYMSNERRVLSIGMDCSEKENKEDLE
                     LGREFKINNDSIFKFVDLMNIMVESISDSCMINIDITDLKEAIVGDKGIKYSFEEFED
                     TKSYSEIADILFDRMEYIGEEFISKKGIVFVEGSPEFSIMELNDLISNIQSKVEESYE
                     VIFSLYIKENLNGNIRVGLLYN"
     gene            2476..4410
                     /locus_tag="PC700_003152"
     CDS             2476..4410
                     /locus_tag="PC700_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2582425.1"
                     /translation="MSENIRGTEYWLMEEFFYQETDTPQIFIEESIIALGFDIKINLK
                     KVFSMGKKSEKYINENCNDKDFIKKFMSIKKGDYFLYKAYQNIEEKVKCNVSIGIVEE
                     DFENGYELSHILGHTLPVKWMHNFDTVSNCKVYTSEFYKLNNNDISSYLEIIKSQDKK
                     LLRKRIEESENNKWYLKNYYYKNVDKCVDDEELFIQPINLEQLESLKNIKTDDYILLY
                     NSEKVICENDHTMKSLFGVCQVMEGFDKEKVSALDGGYFFPVRWIEYGKENIDGNAWV
                     IFENNSIVEKYVKRFTNKMPLNTILYGPPGTGKTYNTKQMVYNLVSETSLRNKYYTGY
                     LDYKNNVEFCTFHQSYGYEEFIEGLKSDGEGNFKPEDGILKDISIEACYNALKFEKKM
                     SFELEKDSLTLETIKDRKKKLVLENIEYSESFNFEESENYVLIIDEINRGNVSKIFGE
                     LITLLEDDKRLTKSNQTIVKLPYSKEKFSLPPNLYIVGTMNTSDKSIALLDIALRRRF
                     NFEEIMPDYDLLSIVDGIDIRKMLYTINKRIEFLYDRDHVIGQAYLLAVENINDIVVV
                     FRSKIIPLLQEYFYYDSEKVGLILGGIGRNEKDKYIVYKEKLIASELFKNNNASIMES
                     KVNYCIKQNISSEELKNIYE"
     gene            4403..>5603
                     /locus_tag="PC700_003153"
     CDS             4403..>5603
                     /locus_tag="PC700_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HDF2582426.1"
                     /translation="MSKNIYVKETYEWIRVGNGENELTEIEYEKLLKYLENNNDVLKS
                     NIIDIKYKKIRFINYVGIICFENVILEILPKLSLSDNLVKDREILLQMLSICNKIPIT
                     MNEKIRLSLKNYNLLNFFVMYFIESMQTQMKKGIYFEYINKIENLNVMRGKILLSTYA
                     KEKGISPMKIRCEYDECSENNFLNQVLKKACISILCRINDNSIQGKIKKILSYFQNVD
                     LIYIDRKKLLGYKFYKNNDRFKDCYLLARLLLLNLSMDNSQNNQEAFSILFEINTLYE
                     EYIGILIKSIWDNSFRETYIQDKSKFLLKNEQTGKKNFNLRPDIVLYDLKNEYEIIID
                     TKWKAIEVDSNVFYRSSDIYQMYAYITAYENAKRCILLYPCIQKDKNYSSWKLSESFK
                     GKFIEAKT"
BASE COUNT         2341 a          428 c          963 g         1871 t
ORIGIN      
        1 aatgaatttg gaaaatagtt cgctaaaata atttgttttt ataaaattac tgtttaagtt
       61 tatagatttt gtataaaata aagttatgtt attaattttt ttagaaataa aacttttact
      121 aggagacaga tatgagaaaa aatagtatta atatagcact aactgcagca attacagcaa
      181 tctatgcagt tttaacatta tcattgggat ttataagtta tggaccaata caatttaggg
      241 tagcagagat tatgatgctt ttggcatttt tagataaagg atatatagta ggtcttacat
      301 taggttgttt tttagcaaat gtaataggac catatggtgt acctgatata atttctggaa
      361 cattagccac atttataagt gcaagtatga tttatattac tagaaagtta ggtaaacaaa
      421 ataaaagtac tttagttata gcttctttat ggccaactgt tataaattcg attgtggttg
      481 gtctaatgtt aaatgtattc tttgggcttc cattaattct atctatgata caggtaggtt
      541 ttggagaatt tgtagtagta acaattgtag gagtaccatt tttctgcttt ttaatgaaaa
      601 aatataaaaa tgtcattaat attaaatttt aaatagtgta tagtaattta attaattgag
      661 agtattatta gtgaaaagag gtgtttattt gtcgaaggta aaaagattga ctgatgtttg
      721 gatatatatt aatgataagc aaaagtttac aataaaagag ttgtcagagg aatttaatct
      781 ttcagctaaa acaatacaac gatatttaaa agaattaaat aaaatgggac ttccaattca
      841 agcagaacaa ggtagaaatg gaggttatag ggtgctaaat aatagctata tacctcctgt
      901 tatatttaca gaaaaagaag ttatgattat actatttgca cttaagtcac ttcagattta
      961 taattataag ttaatggaaa tagaaattaa ttctataatg agaaaagtaa gttatgaacg
     1021 taaaagcagt ataaaagtag gtatagaaaa tattaaaaga tacattggct ttgtagctaa
     1081 tgatactaat gatggagatt ttaagagtag taatgtaact gagtttttta gagcttcttc
     1141 agaatctcta attttaaata ttaagtataa gtgtggtaat cagattttgg aaaaaaacgt
     1201 atgccctata ggtatatatt taaataaggg agcatggttt tcaccagcat atgattgtaa
     1261 aagtgataaa ataatattag ttcatataga taatataatt gatataaaaa gaacaggaga
     1321 aagtaaaaaa atacatataa acttggaaga atggttacat gaagtatata acaatttata
     1381 tggaaatagt tcaactacta aaaaatttgg tgaaattatt tatctaaatg tattattaac
     1441 taaagaaggt gtttctaaaa taaaggacag ttcttggaat ttagatatgg agctaaatga
     1501 agatggtagt ggaataatta gtacattcat aaaggttgaa gatatagatt gttatgcttc
     1561 gattttattt tttattggaa ataatgctaa ggttatagag ccaaaatttt tgaataagct
     1621 actttatgat aaggccaatg agttaagata tttttatgaa gaaaatatgg tataatattt
     1681 agaaatgttg aaattaaata attattaagg agatatacgt tataatggaa aattttaata
     1741 agaatgctaa aataattggt attggagttg aaggtattaa tataataaat gaagtagaag
     1801 aaaaaataaa agcaaatatg gatatagaaa aaataaacat aaatcaagag attgaaaaag
     1861 aatatgttag aagtctttta gatggtgtag atatattatt tttgatatat tcatctgaag
     1921 ataaacatat tagagatatt ataaaagctg tttcatatat gtcaaacgaa agaagagttc
     1981 tatctatagg aatggattgt tcagagaaag aaaataaaga agatttagag cttgggagag
     2041 agtttaaaat caataatgat agtatattta aatttgttga tttaatgaat atcatggtag
     2101 agtctatttc tgattcttgc atgataaata tagatattac tgatttaaaa gaagctattg
     2161 taggagataa aggaattaag tattcttttg aagagtttga agatacaaag tcatatagcg
     2221 agatagctga cattttattt gacaggatgg aatatattgg tgaggaattt ataagtaaga
     2281 aaggaattgt atttgttgaa ggaagtccag aattttctat aatggaattg aatgatttaa
     2341 taagtaatat tcagagcaag gtagaagaaa gctatgaagt aatattttct ttatatataa
     2401 aggaaaattt aaatggaaat attagagtag gcttattgta taattagtat attttagata
     2461 ttaaggagag tgtatatgtc tgaaaatata agaggtacag agtattggtt aatggaagaa
     2521 tttttttatc aggaaacaga cacaccacag atttttatag aagaaagcat aattgcactt
     2581 ggatttgata taaaaataaa tttaaaaaaa gtattcagta tggggaaaaa atcagaaaag
     2641 tatattaatg agaattgtaa tgataaagat tttataaaaa agtttatgtc gattaaaaaa
     2701 ggagattatt ttttatataa agcatatcaa aatatagaag aaaaagttaa atgtaatgtg
     2761 tctattggca tagttgaaga agattttgaa aatggatatg aattaagtca tattttaggg
     2821 catacactac cagtaaaatg gatgcataat tttgatacag tttcaaattg taaagtatat
     2881 acaagtgaat tttataaatt aaataataat gatattagtt catatttaga aattattaag
     2941 tcacaagata agaaattatt gagaaaaaga atagaagaaa gtgaaaataa taaatggtat
     3001 ttaaaaaact attattataa aaatgtagat aagtgtgtag atgatgaaga attatttatt
     3061 cagcccatta atctagaaca attagaaagc ctaaaaaata taaagacaga tgattatatt
     3121 ttattgtata atagcgagaa agtaatttgt gaaaatgacc atacaatgaa gagtctattt
     3181 ggcgtatgtc aagttatgga agggttcgat aaagagaaag taagtgcttt ggatggtgga
     3241 tatttttttc ctgttagatg gatagagtat ggaaaggaga atatagacgg aaatgcatgg
     3301 gttatatttg aaaataatag tatagttgaa aagtatgtaa aaagatttac taataaaatg
     3361 ccattaaata ctatacttta tggaccacca ggaactggta aaacatacaa tacaaaacag
     3421 atggtttaca atctagtaag tgaaaccagt ctaagaaaca aatactatac aggttactta
     3481 gattataaaa acaatgtaga attttgtaca tttcatcaat catatggata tgaggaattt
     3541 atagagggtc ttaagtctga tggtgaaggc aattttaagc cagaagatgg gatattaaag
     3601 gatatatcta tagaagcatg ttataatgca ttgaaatttg aaaaaaagat gagctttgag
     3661 ttggaaaaag atagcttgac tttagaaact ataaaagaca ggaaaaagaa attagtatta
     3721 gaaaatatag aatatagtga gagttttaat tttgaagaat cagaaaacta tgttttaata
     3781 atagatgaaa ttaatagagg gaatgtatct aaaatatttg gtgagctaat aacattattg
     3841 gaagatgata aaagacttac taaatcaaat caaactattg tcaaactacc atattcaaag
     3901 gaaaaatttt ctctacctcc aaatttgtat atagttggaa ctatgaacac atcagataaa
     3961 tcaatagcac ttttagatat agctttaaga agaaggttta attttgaaga aattatgcca
     4021 gattatgatt tattgtctat tgtagatggt atagatatta gaaaaatgct atatacaatc
     4081 aataaaagga tagagttttt atatgataga gaccatgtta ttggacaagc ttatctactt
     4141 gctgtagaaa atattaatga tatagtagta gtatttagaa gtaaaataat accacttctt
     4201 caagagtatt tttattatga ttcagaaaaa gtaggtttga ttttaggagg aattggaaga
     4261 aatgaaaaag ataaatacat tgtttataaa gaaaaactta tagcttctga gttatttaaa
     4321 aataataatg cctcaataat ggagagtaaa gttaactatt gtataaaaca aaacatatct
     4381 agtgaggagc taaaaaatat atatgagtaa aaatatatat gttaaggaaa cctatgaatg
     4441 gataagggta ggtaatggtg aaaatgagct tacagaaatt gaatatgaaa aattgcttaa
     4501 atatctagag aacaataatg atgtattaaa aagtaatata atagatatta aatacaaaaa
     4561 aattagattt ataaactatg taggcatcat atgttttgaa aatgtaatac ttgaaatact
     4621 tcctaaattg tcattaagtg ataatcttgt aaaagataga gagatactat tacagatgct
     4681 ttctatatgt aataaaattc caattacaat gaatgaaaaa ataagattga gtttgaaaaa
     4741 ttataatctc ttaaattttt ttgttatgta tttcattgag agtatgcaaa cacagatgaa
     4801 aaaaggtatt tattttgaat atataaataa aatagagaac ttaaatgtta tgaggggtaa
     4861 aatattatta agtacatatg caaaagaaaa aggcatttct ccaatgaaaa taagatgtga
     4921 atatgatgag tgtagtgaaa ataatttctt gaatcaagtt cttaaaaaag catgtatatc
     4981 tattttatgt agaataaatg acaattcaat tcaaggcaag attaaaaaaa ttttaagcta
     5041 ttttcaaaat gtagatttaa tttatataga tagaaaaaaa ctgttgggtt ataaatttta
     5101 taaaaacaat gatagattta aagattgtta tttactagct agacttctac ttttaaatct
     5161 atctatggat aatagtcaaa ataatcaaga ggcattttct atattatttg aaataaatac
     5221 tttgtatgaa gaatatatag gaattttaat caaaagtata tgggacaatt catttagaga
     5281 aacatatata caagataaga gtaaatttct tttgaaaaat gaacaaacag gtaagaaaaa
     5341 ctttaattta agaccggata tagttttata tgacttaaaa aatgaatatg aaattataat
     5401 tgacactaaa tggaaagcta ttgaagtaga ttcaaatgtt ttttataggt caagtgatat
     5461 ataccagatg tatgcatata taacagcata tgaaaatgca aaaagatgta tattattata
     5521 tccttgtatt caaaaagata agaattatag ttcctggaaa ttatcagaat catttaaggg
     5581 taagtttata gaagcaaaaa ctg
//
LOCUS       DAMJWP010000222         5556 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.229, whole genome shotgun sequence.
ACCESSION   DAMJWP010000222 DAMJWP010000000
VERSION     DAMJWP010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5556
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.229"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     regulatory      70..172
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            266..1642
                     /locus_tag="PC700_003154"
     CDS             266..1642
                     /locus_tag="PC700_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDF2582427.1"
                     /translation="MQTTNSSTMQKLFPILTNKNVDMKKEIIAGVTTFLTMAYIIAVN
                     PNILSETGMPAGALVTGTCLAAAFGCILMGVVANLPFALASGMGLNAFFAYTVVLQMG
                     VPWEVALTAVFVEGIIFIVLSVSGVREAVVNAIPKNMKLAVTGGIGIFIALIGLVNSG
                     IVIGDQATLIKMGRFTPAVIITCLGLIIIAVLDKKRVKGSILFGIVVSSLLAWAFAFM
                     NPEYAQKLGIYLPGGLFKFESLAPIAGKIDLGYVLHPTNIGGFLVVVCTFLFVDFFDT
                     VGTLVGVCSKANMLDEKGNVPNVGRALLTDAVATTVGAGLGVSTVTTYVESSTGVIAG
                     GRTGWTAITVGILFLAAMFFSPVFIAIPSCATAPALIYVGYLMLGTVKDIEFDNITEG
                     VPAFVTIACMALTYSIGDGLTLGILTYVFVNIFYNIFGAKKAEDKKHVSVVMIVLAIL
                     FIIKLWLL"
     gene            complement(1756..2901)
                     /locus_tag="PC700_003155"
     CDS             complement(1756..2901)
                     /locus_tag="PC700_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2582428.1"
                     /translation="MNTFEQLKISSTLIDGLKKQDITSPTEVQSLVIGNIIQNKDLLI
                     NSQTGTGKTLAYLLPIFEKIDTSKRETQALILAPTHELVMQITNQVELLAKNAELSVT
                     SLALIGEVNIQKQIKNIKAVKPHIVIGSCGRVLDLIKQKKLKSHNIKTIVLDEVDNLL
                     NGKNITCIEDIIRTTLRDRQIIGCSASLTDSTIKICDKLMKEFEIIKTKEKSQINPNI
                     NHSYLLGEIRDKFTFLRKALAATNPKKAIVFVNNEKNIEVLVSKLNYHNYKAIGIFGN
                     MEKEDRKNAINKFKLGKAKILITTDLSARGLDIVDVSHVFNLDFPKSKNEYLHRCGRT
                     SRGNRSGNTISIITKKELDIIKDLQKEFSIVITPKTLQNGELIDIIK"
     gene            3153..3860
                     /locus_tag="PC700_003156"
     CDS             3153..3860
                     /locus_tag="PC700_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2582429.1"
                     /translation="MLKVINVSKSYKNKKVVSSVSFDVNDGELFGFIGHNGAGKTTTI
                     KAIVGIHDFEEGEVLINSKSIKKQPVECKKEMAYIPDNPDLYESLTGIQYLNFIADIF
                     EVAKDKREELIKYYSNKFELNKALGDLISSYSHGMKQKLAIISALIHSPKILILDEPF
                     VGLDPKASFILKEIMREFCDKGGCIFFSTHVLEVAEKICDKIAIIKDGNIIAYGTTEE
                     VKGSNSLESIFMELIEK"
     gene            3907..5502
                     /locus_tag="PC700_003157"
     CDS             3907..5502
                     /locus_tag="PC700_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2582430.1"
                     /translation="MSNLSILIKTNIINEFKLNALKKADSREKQKIMGMILSVVIITG
                     LLIFYVTSISLSLVDILKQINQMEMLLIFGFGLSTLTTIVTSLYKTSSYLFQAKDLDL
                     LTSLPIKESTVLASKILMLVGANYLFSSICMIIPAVVYFFNSDVSITYFPYLILLFLA
                     LPLLPIVASSIIFMFIGQISSRIKYKNLILIIGSIILMLLYTLLMTRLNSMSVEMLKN
                     SVSISETIRKIYPPIYYFVDALKTGSVVSFLAFIAISIIPFFMFVTLFAKKFKDINAK
                     MSEGYKSKTYKFKSQKTSRPSKALLKKEFKRYTSSFIYFLNSSAGLLFLIIGTILILL
                     FGADKVGELLKLNIDFNFIKIQLLGVFFFIIVMNCTTYCSISLEGKNLWILKSSPIDE
                     REIFWSKILMNFLLNAPLSILCLILVSIRLGFEIQFIIVSICLIASFSMFVALMGLLS
                     NLLYPNLDWKNEMAVVKRSASMIITMLVSLAYVIILGGIYVLLGISFLNIYIILASIL
                     TFILDFMIWRIISTKGVRIFKNL"
BASE COUNT         1885 a          646 c          864 g         2161 t
ORIGIN      
        1 tcaatacaat atatacgtat atattgacaa aattaattat ataaattaaa ataatattcg
       61 taaagaaaaa ttcatgtttt gtactgtata acatataatt ttgacaatat gggtcataag
      121 tttctaccgg aataccgtaa atattctgac tatgtatact attatttagt taattaaata
      181 tttaattaac tgaattatga aggatacggc tcagcttatc aactactgtg tcgttttttt
      241 gtaaaattta ataggaggtg ttgttatgca aacaacaaac agtagtacta tgcagaaact
      301 ttttccaatt ttgactaata aaaatgtcga catgaagaag gaaataattg caggagtaac
      361 tactttttta actatggctt atataatagc tgtaaatcca aatattttat ctgagacagg
      421 catgcctgca ggtgcacttg ttacaggtac ttgtttagca gcagcatttg gatgtatttt
      481 aatgggtgta gttgctaacc ttccttttgc tttagcatca ggtatgggtc ttaatgcatt
      541 ttttgcatat acagtagttt tacaaatggg tgttccttgg gaagtagctc ttacagcagt
      601 atttgtagaa ggtattattt ttatagtatt atcagtaagt ggagttcgtg aggctgttgt
      661 aaatgcgata ccaaaaaata tgaaattagc tgttacaggt ggtataggaa tatttattgc
      721 acttataggt ctagttaaca gtggaattgt tattggagac caagccactc ttatcaaaat
      781 gggtagattt acaccagcag taataataac ttgtttagga ttaataatta ttgctgtatt
      841 agataaaaaa agagttaaag gttctatact ttttggaata gtagtgagtt cattacttgc
      901 ttgggcattt gcttttatga atccagagta tgcacaaaaa ttaggtatat atttacctgg
      961 tggattattt aaattcgaaa gtttagcacc aatagcaggt aaaatagatt taggatatgt
     1021 actacatcca acaaatatag gtggtttttt agtagtagtt tgtacatttt tatttgttga
     1081 tttttttgat acagtaggta cattagttgg tgtttgttct aaagcaaaca tgttagatga
     1141 aaaagggaat gttcctaatg taggtagagc cttacttaca gatgcggttg caactacagt
     1201 tggtgcagga cttggagtat ctacagttac aacttatgtt gagagttcaa caggagttat
     1261 cgcaggtgga agaactggat ggacagcaat aacagttgga attttattct tagctgcaat
     1321 gtttttctct cctgtattta tagcaattcc atcatgtgct acagcaccag ctttaatata
     1381 tgttggatat ttaatgcttg gtacagttaa ggatatagag tttgataata taactgaagg
     1441 tgttccagct tttgttacta ttgcttgtat ggcattaaca tatagtatag gtgatggttt
     1501 aactttaggt atattaactt atgttttcgt aaatatattt tataatatat ttggagctaa
     1561 aaaggcagaa gacaaaaaac atgtttctgt agtaatgatt gttctggcaa tattatttat
     1621 aataaaactt tggctattat aataggtatt atttaaaatt ttaatttaat ataaaataaa
     1681 gggctataat ttattatcaa ttgtgatgat aaactacagc tcttttgtat ttgttgtatt
     1741 ttcgtattta ttgtattatt taataatgtc tattagttct ccattttgta aagttttagg
     1801 tgtaattact atactgaact ctttttgcaa atcttttata atatctaatt ctttttttgt
     1861 tattattgag attgtgttac cacttcgatt tcctctagaa gttcgaccac atctatgcag
     1921 gtattcattt ttactttttg gaaaatccaa gttaaaaaca tgacttacat ccacaatatc
     1981 tagtccacga gcagataaat cagttgtaat taaaatcttt gctttaccaa gtttaaattt
     2041 atttattgca ttctttctat cttctttttc catatttcca aaaattccta ttgctttgta
     2101 attatgataa tttagttttg atactaggac ttctatattt ttttcattat ttacaaatac
     2161 aatagctttt tttggatttg tagctgctaa tgcctttcta agaaaagtaa atttatccct
     2221 tatctcacct aataaatatg agtgattgat gttaggattt atttgtgatt tttcctttgt
     2281 ttttattatt tcaaactcct tcataagttt atcacaaatt tttatagtgc tatcagtaag
     2341 acttgcagag catcctataa tttgtctatc tcttaacgtt gttcttatta tgtcttctat
     2401 acaagttata ttttttccat tcaatagatt gtccacttca tctaagacta tagtttttat
     2461 attatgtgat tttagcttct tttgttttat caagtctaaa actcttccac aagagccaat
     2521 tactatgtgt ggttttactg cttttatatt ttttatttgt ttttgtatat tgacctcccc
     2581 aattaaagct aaagaagtaa cggagagttc tgcattttta gccaataatt caacctggtt
     2641 tgtaatctgc attactaatt catgcgtagg tgcaagtata agagcttgtg tttctctttt
     2701 tgatgtatca attttttcaa atataggtaa aaggtacgct agtgtttttc ctgttccagt
     2761 ctgtgaatta atcaataaat ccttattttg aattatattc cctataacaa gtgactgaac
     2821 ttctgttgga gaagttatgt cttgtttttt aagtccatct attagagtgg aacttatttt
     2881 taattgttca aatgtattca tattttgctc cttatattta ataattatag tcaactattt
     2941 tttattatac attgtttatt caaatcgaac tatatattct tttataatta tgtgtaattt
     3001 gaaagtaaat atcaattata tttatacctt tatatttctg atacctttgc attttataga
     3061 tttctaaaat aaacaaataa aagttgtaat tgtttgatat ttgttatata ttatatataa
     3121 caaatatcaa aactataata tggagggaaa ttatgttaaa agtaatcaat gtatctaaat
     3181 catataaaaa taaaaaagta gtaagtagtg tctcatttga tgtaaatgat ggagagcttt
     3241 ttgggtttat aggacataat ggagcaggta aaactactac tattaaagca attgttggaa
     3301 tacacgattt tgaagaggga gaagtactaa taaattctaa atctataaaa aaacagcctg
     3361 ttgaatgtaa aaaggaaatg gcatatatac ctgataatcc agatttatac gagtcattaa
     3421 cgggtataca ataccttaat tttatagctg acatatttga agttgccaaa gacaaaagag
     3481 aagagttaat aaaatattat tcaaataagt ttgaactcaa taaagcatta ggagatttaa
     3541 tatcttcata ttcgcatggt atgaaacaaa aattagctat aatttcggct ttgatacaca
     3601 gcccaaaaat cttaattctt gatgaacctt ttgtaggtct tgacccaaaa gcttccttta
     3661 tattgaagga aattatgagg gagttttgtg acaaaggtgg ttgtatattt ttctcaacac
     3721 acgttttaga agttgctgaa aaaatttgtg ataagattgc cataataaaa gatggaaata
     3781 taattgctta tggaactaca gaagaagtta agggaagtaa ttcacttgaa agtatcttta
     3841 tggaattaat agagaaataa aatgtttttt atacaatata gtgtctaaag tgaggaatga
     3901 taaaatatga gcaatctaag tatattaata aaaactaata taataaatga atttaaatta
     3961 aatgcgttaa aaaaagctga tagtagagaa aaacaaaaaa taatggggat gatactttca
     4021 gttgttatta taacagggtt attgattttt tatgtaacta gtataagtct ttctttagtt
     4081 gatattttaa agcaaataaa tcagatggaa atgctactta tatttggatt tggattatca
     4141 acattaacaa ctattgttac atcattatat aaaacgtcat catatctgtt tcaagcaaaa
     4201 gatttagacc ttttgactag tttacctata aaagaatcta ctgtactagc aagtaagata
     4261 ttaatgttgg ttggagcaaa ctatttattt tcttcaatat gtatgattat accagcagtg
     4321 gtttattttt ttaattctga tgtaagtata acgtatttcc cttatttgat actgctattt
     4381 ttagctttac cattattacc aatagttgct tcatcaataa tttttatgtt tataggacag
     4441 atatcatcaa gaataaaata caaaaattta atattaatta taggtagtat tatattgatg
     4501 ttgctttata ctctacttat gacaagactt aactctatgt cggttgagat gttaaagaat
     4561 agtgtatcta tttcagagac gattagaaaa atatatccac ctatatatta ttttgttgac
     4621 gctcttaaaa caggaagtgt agtttccttt ttagcattta tagcaatttc aataatacca
     4681 ttttttatgt ttgtaacatt atttgcaaaa aaatttaaag atataaatgc taagatgagt
     4741 gaaggatata agtcaaaaac ttataaattt aagagccaaa aaacttcaag accttcaaaa
     4801 gctctattaa aaaaagaatt taagagatat acttcttcat ttatatactt tttaaattca
     4861 tctgcaggtt tgttattcct aataataggt acaattttaa tattattatt tggagcagat
     4921 aaagtgggtg agttactgaa attgaatata gattttaatt ttataaaaat acaattattg
     4981 ggagtattct tctttataat agtaatgaat tgtacaacat actgttcaat ttctcttgaa
     5041 ggaaaaaatt tatggatact gaaatcttct ccaatagatg aaagagaaat attctggtca
     5101 aaaatattga tgaatttttt attgaatgca ccattatcta ttttatgttt aatattagta
     5161 tctataagat taggttttga aattcagttt attattgtat caatctgttt gattgcaagt
     5221 ttttcaatgt ttgtggcatt gatgggatta ttatcaaatt tattatatcc aaaccttgat
     5281 tggaaaaatg agatggctgt agtaaaaaga agtgcaagta tgataattac aatgttagta
     5341 tcattagctt acgtaattat acttggaggt atatatgtat tacttggaat ttcgttttta
     5401 aatatatata ttattttggc aagtatactt acttttatat tagattttat gatatggaga
     5461 attatttcta ctaaaggagt aagaatattt aaaaatttat aggtgtattt atgtcaaata
     5521 aagacttatc ttttttagtc ttgcaaataa taggtt
//
LOCUS       DAMJWP010000223         5524 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.230, whole genome shotgun sequence.
ACCESSION   DAMJWP010000223 DAMJWP010000000
VERSION     DAMJWP010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5524
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.230"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(11..1069)
                     /locus_tag="PC700_003158"
     CDS             complement(11..1069)
                     /locus_tag="PC700_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDF2582431.1"
                     /translation="MNKKKITFIFVILILALILFSFFRSSSQNTKYSKTSYYLGTVNE
                     VTIFNVKESKSDKILNECDSILRYIDNKMSTHIPGSDVSKINDNAGKKFVRVSDDTFF
                     VVKEAIQYSKLSDGYFDITIGPLSNLWAIGTDKAKVPSDSEIQKLLPLIDYKNIILDE
                     KNKSIKLTKENMKIDLGAIAKGYAADKIVAYLKSEDVKKGLVNLGGNIFTLEDGKNDK
                     PFKIGIQDPTSKNGESIGNIETTNKSVVTSGIYERFIEKDGKIYHHMLNPFTGYPFEN
                     NLSSVTIISDKSINCDALSTSTFGLGLEKGMDLINEIDNVDAIFITKDKKVYLTKGIK
                     DNFKLTDKSFSIEKLNKQ"
     gene            1136..1507
                     /locus_tag="PC700_003159"
     CDS             1136..1507
                     /locus_tag="PC700_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HDF2582432.1"
                     /translation="MKKKDILLILGILVVIIACYGIINVINSKNAGNIEIYVDNKLYK
                     TVSINAKEEFKIENRGGYNIVKIHDKGVEIVDASCPDKVCVHTGFINKPSQSIVCIPN
                     RVSIKINTNEKNDNQEDIISK"
     gene            1544..2218
                     /locus_tag="PC700_003160"
     CDS             1544..2218
                     /locus_tag="PC700_003160"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDF2582433.1"
                     /translation="MVNLGNDWDELLKEEFEKDYYLNLRKFLIEEYKTRQIFPNMHNI
                     YEALKHTSYKDTKVLILGQDPYHGDNQAHGLAFSVQPQVKTPPSLLNMYKELKDDLGC
                     FIPNNGYLMPWADQGVLLLNTALTVRAHEANSHKNKGWEIFTDRVISILSEREDPVIF
                     VLWGSNARKKVELIDTSKHYILEAPHPSPLSASKGFFGCKHFSKINEILKKLGKEPIN
                     WQIENI"
     gene            2467..3306
                     /locus_tag="PC700_003161"
     CDS             2467..3306
                     /locus_tag="PC700_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HDF2582434.1"
                     /translation="MNFKKLSNAKVSVMAVVLSVGILSGYSALNKQVTLVVRGEERTI
                     STFSSNVGDLLESQNISYDANDIVTLDENTKLSNGDKIEVIDVKEKTVVETKDIPFEV
                     TVVEDGSLLKGDSKVKEEGKSGKSELIYKETYHNGKKVEKEFVKKVVKAEPVNKVVSK
                     GTKVEMQVASSRGESSRRMASSSSSSSSSNNGKNMRVVATAYTGHSITATGTKPKWGT
                     IAVDPKVIPYGTKVYIPQFDMVFTAEDCGGAIKGNKIDIYMNDSKTVYNWGRKTIDIR
                     ILK"
     gene            3549..3645
                     /locus_tag="PC700_003162"
     tRNA            3549..3645
                     /locus_tag="PC700_003162"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3583..3585,aa:Sec,seq:tca)
     gene            3796..4503
                     /locus_tag="PC700_003163"
     CDS             3796..4503
                     /locus_tag="PC700_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HDF2582435.1"
                     /translation="MENNFTTRTSFLVGDDGIEKLNNSNIIVFGVGGVGSFTVEALAR
                     AGVGNITIVDFDDVDITNINRQIPALHSTVGRYKVDVMEERILDINPNINIKKIRSLY
                     NKDTSDEILTERYDYVVDAIDMVSSKIHLIETCEKKGLKIISSMGMGNKLDPTKIVVT
                     DIHKTSTCPLAKVMRKELRDRGIKKLKVVYSTEQPIELKKKVMNGRKVTPGSVSFVPS
                     VGGLIIASVIVNELLGQ"
     gene            complement(4645..5481)
                     /locus_tag="PC700_003164"
     CDS             complement(4645..5481)
                     /locus_tag="PC700_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2582436.1"
                     /translation="MRNKIFLSILIIISLLIGCDKKSLLSIHIIDVGQGDSILVQTPT
                     NKNILIDGGDEDSENIIISYLRQKRIKTIDIIIATHPDSDHIGSLDNVIKKFNVNSIY
                     MPEQSTDSEAYQNLINSCTDKNLSIQHLYKNDVLNIDNNINIYVLSPSYIQEESNLNS
                     IVFKLTFNDNSFLFMGDAEEENEKEILHSFKLNNINFIKIGHHGSNSSSSLEFIKKIS
                     PDIAAISCGYKNQYGHPHREVINNLKQNHVSIYRTDRIGDIVFYSDGEIIFTKYNYEI
                     DT"
BASE COUNT         2097 a          650 c          851 g         1926 t
ORIGIN      
        1 ttatttaaac ttattgttta tttaattttt caatagaaaa agatttatct gtaagtttaa
       61 aattatcttt tataccctta gttaaatata ctttcttatc cttagttatg aatatagcat
      121 ctacattatc aatttcatta attaaatcca tacctttttc aagaccaagt ccaaatgtag
      181 aagtggataa ggcatcacaa ttaatagatt tatcagaaat tattgtaaca ctacttagat
      241 tgttttcaaa aggataccct gtaaatggat ttaacatatg atgatatatt tttccgtctt
      301 tttcgataaa tctttcataa attccagaag taacaacaga tttatttgtg gtttctatgt
      361 ttcctatgga ttcaccattt ttactagtgg ggtcttgaat gcctatttta aaaggtttat
      421 catttttacc atcttctaaa gtaaatatat ttcctcctaa gtttactaaa ccttttttta
      481 cgtcctctga ttttaaatag gctacaattt tatcagcagc atatccttta gctatggcac
      541 ctaaatcaat tttcatattt tctttggtta attttataga tttatttttt tcatccaata
      601 tgatgttctt ataatcaata agaggcagaa gtttttgtat ctcagaatca cttggaactt
      661 tagctttatc tgttccaata gcccataaat ttgataatgg accaattgtt atgtcaaaat
      721 atccatctga taatttggaa tattgtatag cttcttttac gacgaaaaat gtatcatcag
      781 aaactctgac aaatttttta ccagcattat catttatttt agaaacatca cttcctggta
      841 tatgagtgct cattttgttg tcaatatatc ttaaaattga atcacattca tttaagattt
      901 tatcactttt ggattctttt acattaaata tagtaacttc gtttactgta ccaaggtagt
      961 agctagtttt actatattta gtattctggc ttgaactcct aaaaaatgaa aataaaatta
     1021 atgctaaaat caatatcaca aaaataaatg tgattttttt cttattcatg gtatattcct
     1081 cctatgaaat tgtgttatta ttttattata acataatttc ataggaggac aaaaaatgaa
     1141 gaaaaaagac attcttctta ttttaggcat tttggttgtt attatagcat gttatgggat
     1201 aataaatgtt ataaattcta aaaatgctgg aaatattgag atttatgtag ataataaatt
     1261 gtataaaact gtttcaataa atgccaaaga agaatttaaa atagagaata gaggggggta
     1321 taacatagta aagattcatg ataaaggggt tgagatagta gatgcttcat gccctgataa
     1381 ggtttgcgta catactggat ttataaacaa gccaagtcaa agcatagttt gtatacctaa
     1441 tagagtgagc ataaaaataa atacaaatga aaagaatgat aatcaagaag atataatttc
     1501 taaataaaat aataaaaata attgaaagga attggtgata attatggtta acttaggaaa
     1561 cgattgggat gaattattga aagaagaatt tgaaaaagat tactacttaa acttaagaaa
     1621 atttcttata gaagaatata aaacacgcca aattttccca aatatgcaca atatatatga
     1681 agctttaaaa cacacttcat acaaagacac aaaagtctta atattaggac aagaccctta
     1741 tcatggagat aatcaagctc atggtttagc tttttcagtt caaccacaag tgaaaactcc
     1801 accatctcta ttaaatatgt ataaagaatt aaaagatgat ttaggttgtt ttatacctaa
     1861 taatggatat ttaatgcctt gggcagacca aggagtgtta cttttaaata ctgctcttac
     1921 tgttagagcg catgaagcta attctcataa gaacaaagga tgggaaattt tcactgatag
     1981 agtaatatct attttaagtg agcgtgaaga cccagttatt tttgtactat ggggaagtaa
     2041 tgcacgtaaa aaagtagagt tgattgatac aagtaaacat tatattttgg aagcacctca
     2101 tccaagccca ttgtcagcaa gtaaaggctt ttttggatgt aaacacttct ctaagataaa
     2161 tgaaattttg aaaaagctag gaaaagaacc tattaattgg cagatagaaa atatataaaa
     2221 gtaaaaggta acaaaatgat aacaaaacaa caataaacaa tgacattgtt gtaactaata
     2281 gaaatatgct taaatagttg aaaatactat gtatgcatat ttctattttt ttgtcaagtt
     2341 gacaactctt gaaaaaatag ttacaattta gttacacaaa aaacacaaat aaaaaaagaa
     2401 acttacaaag cagaatttta tgaagcaaaa tatatagtgt aaaaaatttt agggaggaaa
     2461 ttttatatga attttaaaaa attgagtaat gctaaagtct cggtaatggc agttgtttta
     2521 tcagttggaa tattatcggg atactctgcg cttaataaac aagtaacatt agtagtaagg
     2581 ggagaagaaa gaactatatc aacatttagt tctaatgtag gagacctttt agaatctcaa
     2641 aacattagct atgatgcaaa tgatatagta actttagatg aaaatacaaa gttatctaat
     2701 ggagataaaa tagaagtaat tgatgtaaag gaaaaaacag ttgtagaaac taaagacatt
     2761 ccatttgaag ttacagttgt tgaagatggc tctttattaa agggtgattc taaggttaaa
     2821 gaagaaggaa agtctggtaa aagtgaatta atatataaag aaacttatca caatggaaag
     2881 aaagttgaaa aggaatttgt gaaaaaggtt gttaaagctg agccagtaaa taaggttgta
     2941 agcaaaggga caaaggtaga aatgcaagta gcctcatcaa ggggagaaag ttcaagaaga
     3001 atggcttcat catcatctag tagcagtagt agtaacaatg gtaaaaatat gagagtggta
     3061 gcaactgcat atactggtca tagtataact gcaacaggaa caaaacctaa gtggggaaca
     3121 atagctgtag accctaaggt tataccatat ggaactaaag tttatatacc tcaatttgac
     3181 atggtgttta ctgcagaaga ctgtggtgga gcaataaaag gaaataaaat tgatatctac
     3241 atgaatgatt caaaaacggt ttataactgg ggaagaaaaa ctatagatat acgtatatta
     3301 aaatagttaa ataatttata tttttactgg aaattatatt ttgattatat agagaagaaa
     3361 ctctttatag attcttaaaa tataatttcc agtttttatt ttggatatgt aataaaacag
     3421 gtaaatttta accatgtata gataaaagct atttaaatta taatttacta tcagataaat
     3481 ctataaaaat tgttgcaaag agtcaaaata tatgatatta tagatttcgt tgagaaggat
     3541 ttcattatgg gagtggatgg gtgccggtgt gccctctagt cttcaaaact agtatgaggg
     3601 gttaagagcc tcttaggtgg gttcgattcc cacgcattcc cgccaatatg caaaaggtat
     3661 cttttaatta aaagatacct ttttaaattt attttaagat aatttatttt taataatata
     3721 taatgttttg tattatataa tgtaaatttt ctaatactat tatatattta gataaaaatc
     3781 taggagtgtg gaaagatgga aaataatttt acaacgagaa ctagttttct agttggagat
     3841 gatgggatag aaaaacttaa taattcaaat ataatagtat ttggagttgg aggagttggt
     3901 agttttactg ttgaagcttt agcaagagct ggagtaggca atattactat tgtagatttt
     3961 gatgatgtag atataaccaa tataaataga caaataccag ccctacattc aactgtagga
     4021 aggtacaaag ttgatgtcat ggaagaaaga atattagata taaatcctaa tatcaatata
     4081 aaaaaaataa gaagtcttta taataaagat actagtgacg aaatactaac tgaaagatat
     4141 gattatgttg tggatgcgat agacatggta agttccaaaa tacatctaat tgaaacttgt
     4201 gaaaaaaaag ggttgaaaat aataagttct atgggtatgg gaaataaatt agacccaact
     4261 aaaatagtcg ttacagatat acacaaaaca agtacttgtc ctctagctaa agttatgagg
     4321 aaagaactta gggatagagg tatcaaaaag ttaaaagtag tatattctac tgaacaacca
     4381 atagaactta agaagaaagt aatgaatggt agaaaagtaa cacctggtag tgtatcattt
     4441 gtcccatctg taggaggttt aataatcgca tctgtaatag taaatgaact attaggacaa
     4501 taagttttaa atattttaat ttataaattt tatatacaaa tttgaaggct agaagcatga
     4561 atataaagca attttatatt catactttta gcctaattat tttatattac cttattttat
     4621 gtagttgtag ttttattttt tagattatgt atctatttca taattatatt ttgtaaatat
     4681 aatttctcca tcactataaa aaacaatatc tcctattcta tctgttctat aaattgatac
     4741 atggttttgt tttagattat taataacctc cctatgtgga tgaccatatt gatttttata
     4801 tccacatgat attgcagcta tatctggact aatttttttt ataaattcta aagatgaaga
     4861 agaattactt ccatgatgac ctatttttat aaaattaata ttatttaatt taaaagaatg
     4921 taaaatttct ttttcatttt cttcttcagc atcacccata aataaaaaag aattatcgtt
     4981 aaaagttaat ttgaaaacta tagaatttaa attagattct tcttgtatat atgatggact
     5041 taatacatat atatttatat tgttatctat gtttaaaaca tcatttttat agagatgctg
     5101 aatagataag tttttatccg tacaagaatt tattagattt tgataagcct cactatcagt
     5161 tgattgttca ggcatatata tggagtttac attaaatttt tttataacat tatcaagact
     5221 tcctatgtgg tctgaatctg gatgagttgc tattataata tcaatagttt ttattctttt
     5281 ttgtctaaga taactaataa ttatattttc gctatcttca tcacctccat caattaatat
     5341 attcttattt gtgggtgttt gaactaaaat actatcacct tgtccaacat caattatatg
     5401 tatagatagt agagattttt tgtcacagcc gattaaaagt gatattataa taagtattga
     5461 taaaaaaatt ttatttctca ataaaaaatc actccattca ttacatgttt atataaattt
     5521 atga
//
LOCUS       DAMJWP010000224         5522 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.231, whole genome shotgun sequence.
ACCESSION   DAMJWP010000224 DAMJWP010000000
VERSION     DAMJWP010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5522
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.231"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            172..1377
                     /locus_tag="PC700_003165"
     CDS             172..1377
                     /locus_tag="PC700_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDF2582437.1"
                     /translation="MSKKVLIMSASTGGGHNRAALAIKEELTSKTLDGEPIECEIIDS
                     LKLVNNTMDKIISRGYEKSAIYTPKAYGSVYRLSETNLLSKNEFKDNLLITFMAKKFK
                     KLIRSEKPDLIIGTHPFPMIALSTLKKNFNLHNNESNAYTEHFYKHYTNTINVPPLIS
                     VLTDYTTHSTWIQNEIDYYIVGHEYVKELLVFDGVGPSKIRTFGIPVEKSFLSHRDKD
                     IVLSELNLSPDKLTVLLMGGSFGAGNIKETLDELLDTDRDFQILVITGKNESLKEKIE
                     KKLMSRYHDKNVCVLGYTDKMNDILASIDVLVSKPGGLTTTEALLKDVPMIVPYYIPG
                     QEEENLDFLSNCGAALRTTKKYNLSILLKVLIDDPSRLEMLKRNVKSIRKSNSAQNIA
                     NLILNIISG"
     gene            complement(1448..1669)
                     /locus_tag="PC700_003166"
     CDS             complement(1448..1669)
                     /locus_tag="PC700_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582438.1"
                     /translation="MKPIMNLEEHNAMKEKRVKKMKSKDKPKYDDFEDISFSNKKKTK
                     KNVRNKNAEFRNNYKNYQYDYEEDFYEPI"
     gene            complement(1810..2409)
                     /gene="recR"
                     /locus_tag="PC700_003167"
     CDS             complement(1810..2409)
                     /gene="recR"
                     /locus_tag="PC700_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDF2582439.1"
                     /translation="MQVYTGPITRLIEEFSKLPGVGRKTAQRLAFHIINMNTNDVEAL
                     SKAIIDAKREIRYCSICCNITDTDPCSMCSNKSRDSSVICVVEDPRDVAAMERTREFK
                     GQYHVLNGVISPMDGIGPDMLKIKELIQRLGNQEVKEIIMATNPTIEGEATAMYIARL
                     VKPMGIKVTRIAHGLPVGGDLEYADEVTISKALEGRREI"
     gene            complement(2422..2772)
                     /locus_tag="PC700_003168"
     CDS             complement(2422..2772)
                     /locus_tag="PC700_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDF2582440.1"
                     /translation="MAKKGFGGGMMPGGGNMNNLLKQAQKMQENMQKAQQELESKEVE
                     ASVGGGAVTVKVNGKKEVIDITIKPEVVDPDDIEMLQDLVLSAVNQALRNIDDIQASQ
                     MSKVTGGMNIPGLF"
     gene            complement(2831..4468)
                     /gene="dnaX"
                     /locus_tag="PC700_003169"
     CDS             complement(2831..4468)
                     /gene="dnaX"
                     /locus_tag="PC700_003169"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDF2582441.1"
                     /translation="MHKALYRAYRPQKFEDVIGQDHIIKTLKNQIYSDNIGHAYLFCG
                     TRGTGKTSTAKIFSRAVNCLNKINEEPCNECEICESVLKDNTMDVVEIDAASNNSVDD
                     IRELRESVKYSPANAKYKVYIIDEVHMLSQGAFNALLKTLEEPPSYVIFILATTEPHK
                     IPATILSRCQRYDFKRVTVKDMTLRMKKICEDEGIDIDDKALNLIARNSQGALRDALS
                     ILDQCMSFGESKIDYKDVVELMGSVNIEQLFELSQCIVEQDTKKSLEILNEFVLWGKD
                     IRNLINDLIDHFRNLMVCKVSSELDEIISLPEETIEQLKIQSKNIDINDLIRILNILS
                     ITQDDIKSSSNPRVLVEITIMKIAQPMFDESKEALIKRVENLEKMIELGNFKSEKIGN
                     NKEKEYEVDREIDVKQENVVYEDVKNEDVILIESSWKNILKQIKKDKKMPIYALLSEV
                     KSFNVYSNMLYVIFDDKFDFAKTRLSSQDTINYLEKTIRDVLNRSFNVKIVLTSEVKD
                     INLEVKEKKDIGEEILKNIVSEEILEIKYSIDENESK"
     gene            complement(4545..4809)
                     /gene="ffs"
                     /locus_tag="PC700_003170"
     ncRNA           complement(4545..4809)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PC700_003170"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(4847..4938)
                     /locus_tag="PC700_003171"
     tRNA            complement(4847..4938)
                     /locus_tag="PC700_003171"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4902..4904),aa:Ser,seq:gga)
     gene            complement(4939..5394)
                     /locus_tag="PC700_003172"
     CDS             complement(4939..5394)
                     /locus_tag="PC700_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HDF2582442.1"
                     /translation="MESSFYMKEALKEAYKAYNKKETPIGAIIVKDNQIIARAHNLTE
                     TLKDSTAHAEILAIKQASEKLGGWRLTDCDLYVTMEPCIMCSGAIVNSRIKKLIIGTR
                     HVKNSYIEKQHEFKLDYFNNNNVKVAFDVLQEECSIILQEFFKALRKRD"
BASE COUNT         1761 a          983 c          635 g         2143 t
ORIGIN      
        1 taacatattt gataaaatta atttacattt ctttcacttg taatttttgc aatcttgtat
       61 tagattaata ttaaaaataa tttatcaata accttattca caaagacagt ttttatatat
      121 caaaatataa tacaatataa atactatgct acattttagg aggattttat tatgagcaaa
      181 aaagtattaa taatgtctgc ttccactggt ggtgggcata atagggctgc tctagctatt
      241 aaagaagaac ttacttccaa aacacttgat ggagagccaa ttgaatgtga aataatagat
      301 agtttgaaat tagttaataa tacaatggat aaaataattt caagaggata cgaaaaatct
      361 gctatatata ctcccaaagc ctatggaagt gtatatagat tatctgaaac aaatcttttg
      421 tcaaaaaatg aatttaaaga taatctacta ataacattca tggctaaaaa atttaaaaag
      481 cttattagaa gcgaaaaacc agacttgatt ataggaacac atccttttcc aatgattgct
      541 ctaagcacat tgaaaaaaaa ctttaattta cataataatg aatcaaatgc ttacacagag
      601 catttttata aacattatac aaataccatt aatgttcctc cgcttatttc tgtattaacc
      661 gattatacta ctcattctac atggatacaa aatgaaattg attactatat tgttggtcat
      721 gaatatgtaa aagaattatt ggtgtttgat ggagttggac cgagtaaaat tagaacattt
      781 ggaatccctg ttgaaaaatc ttttctctct cacagagata aagatattgt tctttctgag
      841 ttgaatttat ctcctgataa attaactgtt ttacttatgg ggggtagctt tggggctgga
      901 aatataaaag agacacttga tgaattactg gatactgata gagattttca aatacttgta
      961 attactggta agaatgaaag cctaaaagaa aaaatcgaaa aaaaacttat gtctcgttat
     1021 catgataaaa atgtatgtgt tcttggatac actgataaga tgaatgatat tttagcttct
     1081 atagatgtat tagtaagcaa acctggtgga cttactacaa ctgaggctct tttaaaagat
     1141 gtacctatga tagttcctta ttatatacca ggtcaggaag aagagaatct tgatttctta
     1201 tctaattgtg gtgcagcact tagaactaca aaaaaatata acttgtcaat attactaaaa
     1261 gttctaatag atgaccctag cagattagaa atgctaaaaa gaaatgttaa atcaataaga
     1321 aagtcaaatt cagcccaaaa tatagctaat ttaattttaa atataatttc tggttgataa
     1381 aataaataaa atccctatag tatatatatt atctattagg gattttttat tttctaatta
     1441 tttaatatta aattggttca taaaagtctt cttcataatc atactgataa tttttataat
     1501 tatttctaaa ttcagcattt ttatttcgta catttttttt agtttttttc ttattagaaa
     1561 aacttatatc ctcaaaatca tcatatttag gtttatcttt tgatttcatt tttttaactc
     1621 ttttttcttt cattgcatta tgttcttcta agttcataat tggtttcatg ttgcacccct
     1681 atcaaaattt ttaaatgatt tatacaacaa attatacaat acagaagaaa tttattcaat
     1741 aaatctaaat caaaattaat ttgaatttaa ctcaaaaaac tacaaaaatc gaggtattaa
     1801 cctcgatttt tatatttctc ttctgccttc tagtgctttt gatattgtga cctcatctgc
     1861 atactctaaa tcgcctccaa caggaagacc atgagctatc cgagtgactt tgattcccat
     1921 tggttttact aacctagcaa tatacatggc tgtcgcttca ccttcaatag taggatttgt
     1981 tgccattatt atttctttga cttcctgatt gccaagtctt tggataagtt cctttatttt
     2041 taacatatct ggtccaattc catccattgg agaaattact ccatttaata catgatactg
     2101 tcctttaaac tctctcgttc tctccatagc tgctacatct cttggatctt ccacaacaca
     2161 tataacactt gaatctctac ttttatttga acacatgcta catgggtctg tatctgttat
     2221 attacaacaa attgagcaat atcttatttc tctttttgca tctataatcg ctttagataa
     2281 agcttccaca tcattagtat tcatattaat aatatgaaaa gccaatcttt gagcagtctt
     2341 tcttcctact ccaggaagct ttgaaaattc ctctataagt ctcgttatgg gacctgtata
     2401 aacttgcata aataccacct actagaataa cccaggtata ttcatacctc cagttacctt
     2461 actcatttga cttgcttgta tatcatcaat atttctcaat gcttgattta ctgcacttaa
     2521 aactaaatct tgtagcattt ctatatcatc tggatcaact acttctggct tgatagttat
     2581 atctataact tcttttttac cattaacttt tacagtaact gctcctccac caactgaagc
     2641 ttctacttct ttactttcta actcttgttg agctttttgc atattttctt gcattttttg
     2701 agcctgcttt aacaagttgt tcatatttcc accaccaggc atcattccgc ctccaaaacc
     2761 ttttttagcc ataaacacta ttcctcctaa acttattttc attttattat tatatcataa
     2821 tagtatttta ttatttactc tcattttcat caatactata ttttatttct aatatctctt
     2881 cactcactat gttctttaat atctcctcac caatgtcctt tttctcttta acttcaagat
     2941 ttatatcttt aacttctgat gtcagtacta tttttacatt aaaacttcta tttaaaacat
     3001 ctcttattgt tttttctaaa taatttattg tatcttgaga gctcagtcta gtttttgcaa
     3061 aatcaaattt atcatcaaat ataacataca acatattaga atatacatta aaacttttta
     3121 cttcacttaa gagtgcgtat attggcattt ttttatcttt tttaatttgt ttaagtatat
     3181 tcttccaaga tgactctata agtataacgt cctcattttt tacatcttca taaactacat
     3241 tttcttgttt tacatctatt tctctatcga cctcatactc tttttcttta ttattcccaa
     3301 ttttttcaga cttaaagttc cccagttcaa tcattttttc taagttctca actcttttta
     3361 taagagcttc tttactttca tcaaacattg gttgagcaat tttcattatg gttatttcca
     3421 ctaaaaccct tggatttgaa gatgacttta tatcatcctg agttattgat aatatattta
     3481 aaatccttat taaatcattt atatctatat ttttagactg aattttaagt tgttctatag
     3541 tttcttctgg taatgaaatt atttcatcca attctgatga aaccttacat accattaaat
     3601 ttctaaaatg atcaattaag tcatttataa gatttcttat atctttgccc cataagacaa
     3661 attcatttaa tatctcaaga gactttttag tgtcctgctc tacaatacat tgagacaatt
     3721 caaataattg ttctatattt acgcttccca ttaattcaac aacatcttta taatctatct
     3781 tactttctcc aaaagacata cattggtcta atatactaag tgcatctcta agagctcctt
     3841 gagaatttct agctattaaa ttaagagctt tatcatctat atcaattcct tcatcttcac
     3901 aaatcttttt cattctaagg gtcatatctt taactgtaac tcttttaaaa tcgtatcttt
     3961 ggcatcttga taaaatggtt gcaggtattt tgtgaggctc tgtagttgct aatataaaaa
     4021 ttacatatga tggaggttcc tctaaggtct ttaataatgc attaaaagct ccttgagaca
     4081 acatatgaac ttcatctatt atatatactt tatatttagc attggctgga gaatatttca
     4141 cactttctct aagctctctt atatcatcta cactattatt tgaagctgca tctatttcta
     4201 caacatccat tgtattatct tttaatacag attcacatat ctcacactca ttacacggtt
     4261 cttcatttat tttattcaaa cagtttactg ctcttgaaaa aatctttgca gtagatgttt
     4321 taccagtacc tcttgttcca caaaacaaat aagcatgtcc tatattatca ctatatattt
     4381 gattttttaa agtcttaata atatgatctt gaccaataac atcttcaaat ttttgtggtc
     4441 tatatgctct atataatgct ttatgcatct ttttacctct ctttagcaaa attaataaaa
     4501 aaatccctca ttatctaagg gattagtctt ttatatatta aatgctgtac accatctatc
     4561 gacttaattc cataggcgtt accaaaacag ttagctcaac ttaggcaccc ccacggcaca
     4621 caaaagtgat cacttaccgc tgcttccttc cggatctgac ggggttcatg agctttcatt
     4681 gcgcaggacc taagcatcaa caccacttat ttagggcggc cctacagaaa ataagccttt
     4741 agatggaatt caatcctgct atagcggatt gcaggttaca gggcaccgct acctccccat
     4801 ctagtacagc aaaattacaa ccatatttaa ttactttcat tgtttctggc ggagagagtg
     4861 ggatttgaac ccacgataca gttttaccca tatactcgct ttccaggcga gcgccttcga
     4921 ccactcgacc atctctcctt aatctctttt tctcaatgcc ttaaaaaatt cttgcaatat
     4981 gatcgagcat tcttcttgca aaacatcaaa agctactttt acattattat tattaaaata
     5041 atctaattta aactcatgct gtttttctat atatgaattt tttacatgtc ttgtccctat
     5101 tataagcttt tttattctag aattaactat tgcgccacta cacattatgc aaggttccat
     5161 tgtaacatat aaatcacaat cagtcaacct ccaaccacct aatttttctg atgcttgttt
     5221 tatcgctaaa atttctgcat gtgccgtaga gtcttttaac gtttctgtaa ggttatgagc
     5281 tctagctatt atttgattat ctttaactat aatagcacct attggtgttt cttttttatt
     5341 atatgcttta tatgcttctt ttaatgcttc tttcatataa aatgaacttt ccataattcc
     5401 tcccaaaact tataacaaac ttatcttatc ataattcatt ttataattca acttaatttt
     5461 attttgttta tttaattatt actatatata gcgatatttt ttttaattag ttctaaaata
     5521 at
//
LOCUS       DAMJWP010000225         5518 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.232, whole genome shotgun sequence.
ACCESSION   DAMJWP010000225 DAMJWP010000000
VERSION     DAMJWP010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5518
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.232"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(146..1087)
                     /locus_tag="PC700_003173"
     CDS             complement(146..1087)
                     /locus_tag="PC700_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HDF2582443.1"
                     /translation="MSKMATREAYGKALVKLGQINDNVVVLDADLSKSTKTHDFYKSF
                     PDRFFNMGIAEQNLIGAACGLSTAGKIPFASTFAMFATGRAFEIIRNSVCYPKLNVKI
                     CATHAGLTVGEDGASHESVEDIAIMRAIPNMTVLVPADGVETEKIIFEIAKYNGPVYV
                     RLGRSSVPVLFDEDYKFEIGKGTVLREGKDVSIIACGIMVNEALLAQEKLQEEGINAR
                     VINMSSIKPIDKDLILESAKETNAIVTVEEHSIIGGLGSAVSEVVGESCPTIVKKVGI
                     KDTFGESGTPNELLKKYELTCDDIIKTVKEAIIAKRM"
     gene            complement(1080..1907)
                     /locus_tag="PC700_003174"
     CDS             complement(1080..1907)
                     /locus_tag="PC700_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDF2582444.1"
                     /translation="MRDHKGLNEIARIIRRDIVSMIHRAKSGHPGGSLSVVEILTALY
                     FDEMNVDSSNPKMEDRDRFVLSKGHAAPALYATLAEKGYFDKEELNGLRKIGRMLQGH
                     PDMKGTPGVEISTGSLGQGFSVACGMAMASKLDNAPWNVYTLLGDGEVQEGIVWEAAM
                     SAAHYKLDNLIAFLDNNGLQIDGDIESVMSLGSIVDKFKAFGWNVIEIDGHDFDQIFA
                     ALDIAKSTVKKPTMIVAKTIKGKGISFMENQAGWHGTAPSDEELEKALLELGGADNE"
     gene            complement(2078..2461)
                     /locus_tag="PC700_003175"
     CDS             complement(2078..2461)
                     /locus_tag="PC700_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDF2582445.1"
                     /translation="MKGRLPKVSTNCEIKRGDLYYADLSPVVGSEQGGVRPVLIIQND
                     VGNKYSPTVIVSAITSQINKAKLPTHVEISSNEYGLNKDSVILLEQIRTIDKKRLREK
                     IGCLDENMMIKVDDGLQISLGLFTF"
     gene            complement(2430..2717)
                     /locus_tag="PC700_003176"
     CDS             complement(2430..2717)
                     /locus_tag="PC700_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HDF2582446.1"
                     /translation="MYDKSKEKIEVTLPDSLISEVDSIVQMENSNREDFAKAAFQFYI
                     TQKKKIHIKESMKTGYREMGQINLSLAELGMTVEEVSSDDKNEGKIAQGEY"
     gene            complement(2750..3907)
                     /gene="alr"
                     /locus_tag="PC700_003177"
     CDS             complement(2750..3907)
                     /gene="alr"
                     /locus_tag="PC700_003177"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDF2582447.1"
                     /translation="MQKITVPTWAEINLDNLRFNLNNIKNLLEEDIKICGVIKADAYG
                     HGAVEVAKLLEKEKVDYLAVARTAEGIELRQNGITLPILNLGYTPDEAFEDSIKNKIT
                     MTVYSLETAQKINEIAKSLGEKACVHVKIDSGMTRIGFQPNEESVQEIIELNKLEYID
                     LEGMFTHFATADEVSKEYTYKQANNYKFMSDKLDEAGVKIAIKHVSNSAAIMDCPDLR
                     LNMVRAGIILYGHYPSDDVFKDRLELRPAMKLKSKIGHIKQVEPGVGISYGLKYTTTG
                     KETIATVPIGYADGFTRIQKNPKVLIKGEVFDVVGRICMDQIMVRIDKDIDIKVGDEV
                     ILFGEGEVTAERIAKDLGTINYEVLCMISRRVDRVYMENNELVQINSYLLK"
     gene            complement(3922..4512)
                     /locus_tag="PC700_003178"
     CDS             complement(3922..4512)
                     /locus_tag="PC700_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582448.1"
                     /translation="MRKKWTIVCIMFLALVIIVIGCQKRQSTKEEVYKDFQKQISDMN
                     YYSCKAEVEVVGNKSPHNYVLIHTYKKTDNYKLEVISPKHLKGKSIEYQGDKILVKNP
                     KISDVVELPNTGKNNQYLFVGDFIKNYLQNEEMKVKLSKGHLVLETFIPGDNKYFNKQ
                     VLYVNADTKNPEKMEVLDKEGVPRFTVKYKDFEYRN"
     gene            complement(4533..4994)
                     /locus_tag="PC700_003179"
     CDS             complement(4533..4994)
                     /locus_tag="PC700_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDF2582449.1"
                     /translation="MMDKTAKEIMTTDVIVAKQDDSIADVANMLIAEKIGGLPVVDSE
                     NRVVGIISETDILKKEKYIEAPLYINLLQGLIFLDDLKKVEKDIKQVAAYKVGELMSK
                     DIIKVHEDDKFDDVANVMIKKSINRVPVVDDDNKLKGIICRYDIIKALYNE"
     gene            complement(5009..5389)
                     /locus_tag="PC700_003180"
     CDS             complement(5009..5389)
                     /locus_tag="PC700_003180"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDF2582450.1"
                     /translation="MNIFDIGVDIIEIDRIRKAVDKNNRFLEKIFTDREIEYFNSKNF
                     KAESIAGNFAAKEAISKSIGTGIRLFNFKDIEVLRNEMGKPIVKTYNNLAKMCIDYNV
                     LEIKVSISHSKDYAIANAITIIKD"
BASE COUNT         1649 a         1086 c          578 g         2205 t
ORIGIN      
        1 tatttttgta aaaatttgaa tattttatta catttttgat atattgatat catataaaaa
       61 aaagagagat ataaattaat ataacacttt atacctctct tttaaatata ttatattatt
      121 aacaaacatg aataatttat atctcttaca ttctttttgc aataatagct tctttaacag
      181 tctttattat gtcatcacaa gttaattcat atttttttaa caattcattt ggagtaccag
      241 attctccaaa tgtatcttta attccaactt tctttacaat ggttggacaa gactctccta
      301 ctacctcact tacagcagaa cctaatcccc caattatgct atgttcttct acagttacaa
      361 tagcattcgt ttcttttgct gattctaata ttaaatcttt gtctataggt tttatcgagg
      421 acatgtttat aactctagca tttatacctt cttcttgcaa cttttcttgt gctaatagag
      481 cttcatttac cataattcca caagctataa ttgatacatc ctttccttct cttaagactg
      541 taccttttcc tatttcaaat ttataatctt catcaaataa aactggtact gaacttctcc
      601 ctaatctaac ataaacaggt ccattatact tagctatttc aaaaataatt ttttctgttt
      661 caactccatc agctggaaca agaactgtca tatttggtat agctctcatt atagcaatat
      721 cttccacact ttcatgagat gctccatcct ctccaacagt aagacctgca tgagtagcac
      781 atatttttac atttagcttt ggataacaaa ctgaatttct tataatctca aaagcccttc
      841 ccgtagcaaa catagcaaaa gtacttgcaa aaggtatttt accagcagtt gataatccac
      901 aagcagcgcc tattaaattt tgttcagcta tacccatatt aaaaaatcta tctggaaatg
      961 acttgtaaaa atcatgtgtt tttgtagact ttgataaatc tgcatctaat actacaacat
     1021 tgtcatttat ttgacctaat tttactaatg ctttaccata tgcttctctc gtcgccattt
     1081 tactcattgt cagcacctcc taattcaagc aaggcttttt ctagctcctc atcacttgga
     1141 gctgtcccat gccatcctgc ttgattttcc ataaaagata ttcctttgcc ttttatagtt
     1201 tttgcaacta tcattgtagg ttttttaact gttgatttag ctatatcaag agccgcaaat
     1261 atttggtcaa aatcatgtcc atctatctca attacattcc aaccaaaagc tttaaattta
     1321 tcaactattg aacctaaact cattacgctt tctatatctc catctatttg aagtccatta
     1381 ttatctaaaa aagcaattaa attatcaagt ttataatgtg cagcactcat agcagcttcc
     1441 caaacaattc cttcttgaac ctcaccatca ccaagcaaag tatacacatt ccacggagca
     1501 ttatccaact ttgatgccat agccatacca catgctactg aaaaaccttg acctaaagaa
     1561 ccagtagata tttccacacc tggcgtgcct ttcatgtctg gatgaccttg taacatcctt
     1621 cctatcttcc ttaatccatt cagttcttcc ttatcaaaat agcctttttc agccaatgta
     1681 gcatataatg ctggtgcagc atgtcctttt gataatacaa atctatctct atcctccatt
     1741 ttaggatttg aagaatccac attcatttca tcaaagtata acgcagtaag tatttcaact
     1801 actgataatg accctcctgg atgtccagat ttggctctat gtatcattga aactatatct
     1861 cttcttatga tacgcgcaat ctcatttaat cctttatggt ctctcatgca attacctccc
     1921 ttgagttcgt aattttatat caattccata ttattatatc acttttatgc tatttttttg
     1981 cgttattttt aattttatta acatagtaca ttaaaattaa accttaagag atatttaaaa
     2041 gaaaaaaggg attgtctaat aaaagacaaa tccctcttta aaaagtaaac aatcctaaac
     2101 ttatttgaag tccatcatct acttttatca tcatattttc atctagacaa ccaatttttt
     2161 ccctcagtct ctttttatcg atagttctaa tttgctctaa aagaattact gagtctttat
     2221 taagcccata ttcattggaa cttatctcta catgagtagg taatttggct ttattaatct
     2281 gagatgttat agctgatact ataactgtag gactatattt attacctaca tcattttgta
     2341 ttatcagaac aggtctaacg cctccctgct ctgaaccaac aactggactt aaatcagcgt
     2401 aatataaatc accacgcttt atttcacaat tagtactcac cttgggcaat cttcccttca
     2461 tttttatcat cagaagatac ttcttcaact gtcataccta attcagctag tgacagatta
     2521 atttgaccca tttctctata acccgttttc atagattctt taatatgaat tttctttttc
     2581 tgagttatat agaactgaaa agcagctttt gcaaaatctt ctctgttact gttctccatc
     2641 tgaactatac tatcaacttc agaaatcaga gagtcaggta aagtaacttc tattttttct
     2701 ttactcttat cgtacacaaa atcgcctccc agattataat atcatggttt tattttagca
     2761 aataactgtt tatttgtaca agctcattat tttccatata aacacggtca actcttcttg
     2821 atatcataca taacacttca tagtttatag ttcctaagtc tttagctata cgctcagctg
     2881 taacttcgcc ttctccaaat agtataacct catctccaac ttttatgtct atatctttgt
     2941 caattctaac cattatttga tccatacata ttctaccaac tacatcaaac acttctccct
     3001 taataagaac ctttggattt ttttggattc tagtaaatcc atctgcgtat cctattggaa
     3061 ctgtagctat tgtttcttta cctgtagttg tgtattttag tccataactt attcctacac
     3121 ctggttcaac ttgttttata tgtccgattt ttgattttaa tttcatggct ggtcttaatt
     3181 ctaatctatc tttaaataca tcatcagatg gataatgacc atataatatt attcctgctc
     3241 ttaccatatt taatcttaaa tcagggcaat ccataatagc tgcactgttt gatacatgtt
     3301 ttatagctat ttttacacca gcctcatcta atttatcaga cataaattta taattattag
     3361 cttgtttata agtgtactct ttacttactt catcagctgt agcaaaatga gtaaacatac
     3421 cttctaaatc gatatattct aatttattta attctattat ttcctgtact gactcctcat
     3481 taggttggaa acctattcta gtcatccctg agtctatttt aacatgaaca caggcttttt
     3541 ctcctaaaga ttttgcaatt tcatttatct tttgtgctgt ttctaaagaa taaactgtca
     3601 tagttatttt attttttata gaatcttcaa aagcttcgtc tggagtatat cccaagttca
     3661 aaataggaag tgttatgcca ttttgtctaa gttcaattcc ttcagcagtt cttgctactg
     3721 ctaagtaatc tactttttct ttttctagca attttgcaac ttctactgca ccatgtccat
     3781 atgcatcagc ttttattact ccacaaatct taatatcttc ttctaataaa tttttaatat
     3841 tatttaagtt aaatcttaag ttatctagat ttatctctgc ccatgtaggc actgttattt
     3901 tttgcatgtc tttagtcccc cttagtttct gtattcaaaa tctttgtatt ttactgtaaa
     3961 tcttggcact ccctctttat ctaacacttc cattttttca ggattttttg tgtcagcatt
     4021 tacatataat acttgcttgt taaaatattt attatcccca ggaataaaag tctctaaaac
     4081 caaatgtcct ttggataact ttactttcat ctcctcattt tgaagatagt tttttataaa
     4141 gtctcctaca aaaagatatt gattattttt tcctgtatta ggtaactcca ctacatcact
     4201 aatctttgga tttttaacta atattttatc accttgatat tctatacttt tcccttttaa
     4261 atgttttggg gaaattactt ctaatttata gttatcggtt tttttataag tatgaattaa
     4321 aacataattg tgtgggcttt tatttccaac tacttctact tcagctttac aactgtaata
     4381 attcatatca gaaatctgct tttgaaaatc tttgtatact tcttcttttg tggactgtcg
     4441 tttttggcat cctattacaa ttataaccag tgccaagaac ataatacata ctatggtcca
     4501 cttttttctc acatcacacc tcctacttca gtttattcat tgtataaagc ttttattata
     4561 tcatatctac aaattatacc tttaagtttg ttgtcatcat ctactacagg aactctgttt
     4621 attgattttt taatcattac attagctaca tcatcaaact tatcatcttc atgaaccttt
     4681 attatatctt tagacataag ttctccaacc ttataagcag ctacttgttt tatatctttt
     4741 tcaacttttt ttaagtcatc taagaaaatc aatccttgta atagatttat atagagaggt
     4801 gcttctatat atttttcttt tttaagaata tctgtttcag aaattattcc aacaactcta
     4861 ttctctgaat ctacaactgg cagacctcct attttttctg caattagcat attagctaca
     4921 tctgctatac tatcatcttg ttttgctact attacatctg ttgtcattat ttcttttgct
     4981 gttttatcca tcataatgta atcactcctt agtccttaat tatggttatc gcatttgcta
     5041 tagcataatc ttttgaatgt gatattgaaa cttttatttc taaaacatta taatctatgc
     5101 acatttttgc tagattatta taagttttta ctattggttt tcccatttca tttctgagaa
     5161 cttctatatc tttaaaatta aacaatctaa ttcctgtacc tatagattta cttatagctt
     5221 ccttggctgc gaaattacct gctatacttt ctgctttaaa attcttagaa ttaaaatact
     5281 ctatttctct atctgtaaat atcttctcca aaaacctatt atttttgtct acagcctttc
     5341 ttattctatc aatttcaatt atatctacgc caatatcaaa tatattcatt gttcctccag
     5401 aaaatttata atacttcttt caataattaa taatgaattt ataaatttac tatcgataat
     5461 acacattatt atttagttta tgttacttat cttaatttaa taatcattat aataaaac
//
LOCUS       DAMJWP010000226         5503 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.233, whole genome shotgun sequence.
ACCESSION   DAMJWP010000226 DAMJWP010000000
VERSION     DAMJWP010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5503
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.233"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(482..1033)
                     /locus_tag="PC700_003181"
     CDS             complement(482..1033)
                     /locus_tag="PC700_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2582451.1"
                     /translation="MSINTVIAKNLNRLRNERNLSLGQLAELSGVSKVMLSQIEKGDS
                     NPTVNTIWKIASGLNVPYTAILEQPQNETFIVSKTDIDVQVSENKDYRLYCYYPNTPT
                     RNFELFQMELEEGHSYTSVGHSEKSQEYIMIIEGQLKLEVNDSIYQLRENDSICFSAE
                     SIHTYHNQGEKTLKAVIINYYPV"
     gene            1224..2186
                     /locus_tag="PC700_003182"
     CDS             1224..2186
                     /locus_tag="PC700_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HDF2582452.1"
                     /translation="MRVLNNVTEAIGNTHMLHLSKIADEFGVQGNIYAKMEYLNPGFS
                     KKDRVALQMITEAENAGLLVPGQAVVELTSGNTGTGLAIACACKGYKFIACMSKGNSM
                     ERARMMKALGAEVVLVDQMPNSVHGQVSGEDLALVEIEAQKIAKERNAFRADQFNLEA
                     CNHAHEKYTAEEMWEQLDGNIDVFVDFLGSGSTFAGCSKTLKKHNSSIKCYVVEPETA
                     AIYSGKEITDQGHKIQGGGYSMDLPLVDKKLIDGYIQVTDEEATDVARKLAKLEGVFA
                     GFSSGANVCAAIKLLKSSEKGKNIVLTLNDSGLKYLSTDLYEEL"
     gene            2481..4325
                     /locus_tag="PC700_003183"
     CDS             2481..4325
                     /locus_tag="PC700_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDF2582453.1"
                     /translation="MQKRILRNFIFTGKNILSKLVFIIKYNFSTLILFELFHKGLALF
                     FIWPSIRYIFDILIKSLHIVYLNMDNFIKVLTNPMSIVLIILSVIILAFYAFFEFTSV
                     IICFNKSIKYEKIGLFELFKISFKNSIKLLYPKNILLFIFVILIIPLVNTVLISGFIG
                     KIQIPEYILDYIKSDIILNIIYMIFLLIIYISVIRWIFSIHEITLNTENFKRARKESV
                     NLIKRKIIRTIIYSLILSLVVAIIGYAIYHSGIIFIGIWTKYCTDMEFLKNVFINRCI
                     AFEEYFRLILSIFIFILDIGFISATYYQYKGVNISKNNIKKEKKSISKIVFNLVLILL
                     ISYIESVAFSSHINGMFNTSFSFYTTAIAHRAGAEMAPENTLAAIEEASNAKADYAEI
                     DVQETKDGELIVLHDSNFKRTTGVDKNVWDVNYNEVKTYDAGSFYYYGKGYYDEKFVG
                     EKIPTLKEMIKYSKGKVKLLIEIKLNGHEKGDIVKKVVQLIKENNFENQCIVASMDKT
                     ILQKVKKLDENLLTCYFMAIAYGDFSKLNYVDIYGIESTFVNKTVVEKIHKMNKKIFV
                     WTVNSDSLIEKMLDLNVDSIITDNPYLIESAIYWKKNGFISQVSDYLF"
     gene            4647..5231
                     /locus_tag="PC700_003184"
     CDS             4647..5231
                     /locus_tag="PC700_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDF2582454.1"
                     /translation="MTEREKMLAGELYDCGDIELLTQWHKAKDLVRDYNQTDSSDLEK
                     KEKILNELLGGKDKNLWITAPYYVDYGNNIYFGNNCEVNMNCTFLDDNKIIIGDNALI
                     APNVQIYTAFHPTNAGDRFGNVKEDGSFEFCKTQTAPVTIGDNVWIGGGAIIMPGVTI
                     GDNVVIGAGSIVTKDIPSNMIAYGNPCRIIRENK"
BASE COUNT         2142 a          557 c          863 g         1941 t
ORIGIN      
        1 taaatataaa gtgcatatag tgataaaaca aaagtttcat ataaatgaag aaaaaatata
       61 aaataattta tttaactact taaaaataaa ctaaaaggga taataaaata gcttttaggt
      121 tatggtaata aaggtattta aaaagagtgc ataagcactc caattgtgag aaatacatac
      181 acaaatcatt attacaacaa tgaaaaatgt acatataaga gacgttttgt atttgtttta
      241 agtgataata ttttaatact aactaaataa ttatcacaat aataaaaaag aaaaaggagt
      301 attttgtact ccatttgtca aaaatataaa acttttatat aagcttatta taacataatt
      361 gataaaagtg taaagctata taatgaattt ataaagaaaa ataaaataaa aaagataaat
      421 cctaatcaaa ggatttatct ttttgtatag aaaataatta ttctattatt aaatggcatg
      481 gctaaactgg ataataattt attattactg ctttcaatgt tttttctcct tgattatggt
      541 atgtatgaat tgattcagct gaaaaacaaa tagagtcatt ttctctaagt tgataaatag
      601 agtcatttac ttctaatttt aattgacctt ctataatcat aatatattcc tgtgattttt
      661 cagaatgacc aacagatgta taactatgac cttcttctaa ttccatttga aataattcga
      721 agtttctagt tggtgtattg ggataataac aataaagacg ataatcttta ttttctgaaa
      781 cttgtacatc tatatcagtt tttgatacaa taaaagtttc attttgaggt tgttccagta
      841 ttgcagtata tggaacattt agtccacttg caattttcca aattgtgttt actgttgggt
      901 ttgagtctcc cttttcaatt tgcgaaagca ttactttact tacgccagat aattcagcaa
      961 gttgacctag acttaagtta cgttcattac gtaatctatt taaattttta gcaataacag
     1021 tattaatact catagtaact ccttaaaatg taaattattg acaaatataa tttacgaatg
     1081 tataatttat ttaacattat ttataattta caaatgtata atataatgaa cgttaaatac
     1141 actgtacatc cgtaatataa attatactgc ataaagagtt gtttttcaac ggatgatagt
     1201 tgagagatta agggggaatt gtaatgcgtg tacttaataa tgttacagag gctataggca
     1261 atactcatat gttacattta tccaaaatag cagatgaatt tggagttcaa ggtaatattt
     1321 atgcaaagat ggaatactta aatccgggat ttagtaaaaa ggaccgtgta gcattacaaa
     1381 tgattaccga agcagaaaat gcaggtttat tagttccagg tcaagcagtt gtggaattaa
     1441 caagtggtaa tactggaaca ggactagcaa tagcttgtgc atgtaaagga tataaattta
     1501 tagcgtgtat gtcaaaagga aattctatgg aaagagcaag aatgatgaaa gctttaggtg
     1561 ctgaagtagt tcttgtagac caaatgccta attcagttca cggacaggta tctggtgaag
     1621 atttggcact tgtagaaatt gaggctcaaa aaatagcaaa agaaagaaat gcatttagag
     1681 cagaccaatt taatttagaa gcttgtaatc atgcacatga aaagtataca gcagaagaaa
     1741 tgtgggaaca attggatgga aatattgatg tatttgtaga ttttcttgga agtggtagta
     1801 cttttgcagg atgttcaaaa acacttaaaa aacataattc ttctatcaaa tgttatgttg
     1861 ttgaacctga aacagcagca atttattcag gtaaagaaat aactgaccaa gggcataaaa
     1921 tacaaggtgg aggatattct atggatttgc cattagtaga caagaaactt attgatggtt
     1981 atattcaagt tacagacgag gaggcaactg atgtagcaag aaaattagct aaattagaag
     2041 gtgtatttgc aggattttct tcaggggcaa atgtgtgtgc agcaattaag ttgttaaaga
     2101 gcagtgaaaa aggaaaaaat atagttttaa ctcttaatga tagtggttta aaatatttaa
     2161 gtactgattt atatgaagaa ttgtaataat agcacaatag ttatgcaatg ttttttaaat
     2221 taagtatata gatttaaatt ataaaaagta tctcaatgtg atttttgaaa gttataaaag
     2281 agatactttt ttattttgta aatatggagt atcctactta taagtattaa gctatataga
     2341 tattgatgat tagctatgca ataaaaaaga gtatgtgttt ttgcatttat acatcataac
     2401 ttggaataat acatattaat gttctaaatg tatttaaaac atatgataaa attagaacaa
     2461 acagatacaa ggagataaac atgcaaaaga gaattttaag aaattttata tttacgggta
     2521 aaaatatttt aagtaaactt gtttttataa taaaatataa ttttagtaca ttaattttat
     2581 ttgaattatt tcataaagga ttagccttat tttttatatg gccatctatt agatatatat
     2641 ttgatatttt aataaaaagt ttacatatag tctacctaaa tatggataat tttataaaag
     2701 ttttgaccaa tcccatgtca atagttctga taatactatc agttataata ctagcttttt
     2761 atgcattttt tgaatttacc tctgtaatta tttgttttaa taaatcgata aaatatgaaa
     2821 aaatagggtt atttgaactc tttaaaatat catttaagaa ctctataaaa ttgttatatc
     2881 ctaaaaatat actattattt atatttgtga tattaataat tccattagta aatacagttt
     2941 taatttcagg gtttatagga aaaatacaaa taccagagta catattagat tatataaagt
     3001 ctgatataat tttaaatata atctatatga tttttctttt aataatctat atttcggtta
     3061 ttagatggat ttttagcatt catgagataa ctttaaatac agaaaatttt aaaagagcaa
     3121 gaaaagaaag tgttaatctt ataaagagaa agattataag aacaattatt tattcattaa
     3181 tattatcttt agtagtagct attattggat atgcgattta tcatagtgga attatattta
     3241 taggcatatg gactaaatat tgtactgata tggaattttt gaagaatgta tttataaata
     3301 gatgtattgc atttgaagaa tattttagat tgattttatc aatattcatt tttatattgg
     3361 acattggatt tatctcagca acttattatc aatataaagg agtaaatatt tctaaaaata
     3421 atattaaaaa agagaaaaaa tctatatcaa aaattgtttt taatttagta ttaatattat
     3481 tgatttctta tatagagtca gttgcatttt cttcacatat aaatggcatg tttaatacgt
     3541 cattttcatt ttatacaaca gcaattgctc atagagcagg agctgaaatg gctccagaga
     3601 atactttggc agcaatagaa gaagcttcaa atgctaaagc tgattatgca gaaatagatg
     3661 ttcaagaaac taaagatgga gaacttattg tacttcatga ttcaaacttt aagagaacta
     3721 caggagtaga taaaaatgtt tgggatgtaa attataatga agtaaagaca tatgatgcgg
     3781 gaagctttta ttattatgga aaaggttatt atgatgaaaa gtttgttggg gaaaagatac
     3841 caacactcaa agaaatgata aaatattcta aaggtaaggt gaaattatta atagaaataa
     3901 aacttaatgg acatgaaaag ggagacatag taaaaaaagt tgttcagtta attaaagaaa
     3961 ataattttga aaatcaatgt atagttgcat ctatggataa aactatatta caaaaagtaa
     4021 aaaaactgga tgagaattta ctgacatgct attttatggc aattgcatat ggtgattttt
     4081 caaagttaaa ttatgttgat atatatggaa ttgaatcaac ttttgttaat aaaacagtag
     4141 tagaaaagat tcataaaatg aacaaaaaaa tcttcgtttg gactgttaat agtgacagtt
     4201 taattgagaa aatgcttgac ctaaatgtag atagtattat tacagataat ccatacctta
     4261 tagagagtgc aatttattgg aagaaaaatg gatttattag tcaagtgtca gattatttat
     4321 tttaaatatt ctataattgt attatccagt aaaagagata ttattaaaat caataatatc
     4381 tctttttaac ataaataagg ttgttaaatt aaggttattc ttataaaaat tgtgcttatc
     4441 agctataaac aaggaataaa agtgaaaaaa ttgctctgag attattacag ttaaaagagt
     4501 tttaagatga cagagagata gattgtcaac tgtatattta ataaatcttg ttatttatta
     4561 aattttatat aagtaaaata tataaattaa taaatcttat gctagaatat atatgaaaaa
     4621 caatataaat ttggaggatt aaaaacatga cagagcgaga aaaaatgttg gcaggagaac
     4681 tttacgattg tggtgatatt gagttgttaa ctcaatggca taaagcaaaa gacctagtaa
     4741 gagattataa ccaaacagat tcctcggatt tggaaaaaaa agaaaaaata ttaaatgaat
     4801 tacttggtgg taaggataaa aacttatgga ttactgcacc gtattatgta gattatggaa
     4861 ataatattta ttttggaaat aattgtgaag tcaatatgaa ttgtacgttt ttagatgata
     4921 ataaaattat cattggggat aatgcactaa tcgcaccaaa cgtacaaata tatactgcat
     4981 ttcatccaac taatgctggt gatagatttg gtaatgtcaa agaagatggt tcatttgaat
     5041 tttgtaaaac tcaaacagct ccagttacta ttggagataa tgtttggatt ggcgggggtg
     5101 ctattatcat gccaggtgtt actattggag ataatgtagt tataggtgca ggaagtattg
     5161 ttacaaaaga tattccaagt aatatgattg catacggaaa tccatgccgt attattcgag
     5221 aaaacaaata aagttaaatt ttcttgcaaa atctcaaaaa acacctttct tactttatac
     5281 atagggtatt ttccaatact ttcacatcca tattcatttg ttaaattttt gtggaatgta
     5341 gtttttatca cttctatctt acatttccac cattttttat aaaagtcatt cctttataga
     5401 tttatttata atagtatata ttctatttga tgtttgaata agcacttatt tttaagtaaa
     5461 atactaactt ttaggttagt attttgctat agtatgatga tta
//
LOCUS       DAMJWP010000227         5489 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.234, whole genome shotgun sequence.
ACCESSION   DAMJWP010000227 DAMJWP010000000
VERSION     DAMJWP010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5489
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.234"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            129..2630
                     /locus_tag="PC700_003185"
     CDS             129..2630
                     /locus_tag="PC700_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDF2582455.1"
                     /translation="MSSNKVVESYKITGMTCAACAKAVERVTKKMDGVYDQSVNIATE
                     KLKIEYDNSKVNFDDIKQVVEKAGYGIIKEESNKKIDMKIDGMTCAACAKAVERVVKK
                     LDGVESISVNIATDKANIDYDPSKVKLSQIKAAIEKAGYKPIEEVRNKVDVDEDKLRK
                     EREMKSLFVKFIVAIVFAVPLFYIAMGPMIIKPIGPWPLPEIINPMTNTFNYALIQLI
                     LVIPVMIAGYKFYINGFKSLFSLSPNMDSLVAIGTLAAFLYSLYTTLQIANGQIQGMH
                     HHQLYYESAGIIIALILLGKYLESKSKGKTSEAIKKLMGLQPKTAIVLVDGKEVETPI
                     EEVEIGDILLVKPGTKIPVDGVVIEGYTSVDESMLTGESIPVEKNVGSKVTGASINKN
                     GVIKFKAEKIGGDTALAQIIKLVEDAQGTKAPIAKLADTVSGYFVPIVIAIAVVASLL
                     WFLIGGKDIVFVLTIFISVLVIACPCALGLATPTAIMVGTGKGAENGILIKGGEALES
                     AHKVNTVIFDKTGTITEGKPKVTDIVLNNNVKEEYLIKIASSAEKGSEHPLGEAIVKY
                     GEEKNIKFEKVDNFKAIPGAGIQVTINDESILLGNRKLMNDNNIKLGDLEEKSNVLAS
                     QGKTPMYIAVDGNLSGIIAVADVVKESSKKAIEILHDMGIKVAMVTGDNAKTANAIAN
                     QVGIDMVLAEVLPEDKSKEVEKLQNQGKFVAMVGDGINDAPALAKADIGIAIGSGTDV
                     AIESADIVLMKSDLIDVPTAIKLSHETIKNIKQNLFWAFGYNTIGIPVAAGILYVFGG
                     PLLNPMIAAAAMSLSSVSVVSNALRLKNFKAYKRD"
     gene            2790..3458
                     /locus_tag="PC700_003186"
     CDS             2790..3458
                     /locus_tag="PC700_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2582456.1"
                     /translation="MNSSILVIEDDSNIQELISEFLSAEGYQVDTANDGLEGIQKFKQ
                     GSYDLVILDIMMPNLDGYGVCKMIRKSSSVPIIFLTALNDEGDQLKGFDLECDDYITK
                     PFSFNLLIKRVEAILRRSNKTINDKFIVFEKLKLDLNTYIAEIDGEPIELTLKEFNIL
                     KALIEKYPQVITREGLLDSIWGYDYYGDTRIVDAHIKNIRKKISLPYIKTVKGIGYTL
                     EKDI"
     gene            3442..4719
                     /locus_tag="PC700_003187"
     CDS             3442..4719
                     /locus_tag="PC700_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HDF2582457.1"
                     /translation="MRRIFDKWEKLSIKYKLFSITTSLLIALALIIYLILYFLLPSYY
                     HEYKIESLQESLKSLVDSSIHFDTYTLEERLYYMAKDQNLAILLKDNQGKIVYGKNEV
                     VILRYSKYMINSLEDEYRTSIPIYTKDAKDGPYTLELVMPLQPIDEANEVIRKLMPYI
                     ISIAILIAIIGAYIYSIVITKPLINIIESEREQEYRRKDFVATISHELKTPITIISGQ
                     IEGMIYSVGKYKDRDTYLKKSYECTQELKDLVNEMIEVSKSEILEKDLKLVSINISEL
                     LNRLVKRQVFLIEEKHMKTILKIEENLEIKADQERITKAINNIINNAIKYSPEKSELI
                     IRLYDKNKRISKKTSNQRVVLEIENTGVTIEKRYLEEIFNPFYRIEKSRSRKTGGSGL
                     GLYIVSQIFKSHGFDYSIKNKENSVVFTVEFKN"
     gene            5120..5341
                     /locus_tag="PC700_003188"
     CDS             5120..5341
                     /locus_tag="PC700_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582458.1"
                     /translation="MDRLKRDMKNMKEDLKEGVKKTAYVMKNTAEDLKDDAQGAMVNL
                     EMKKDEMMERYEQKKFSKELEKQMKNEMK"
BASE COUNT         2313 a          556 c          976 g         1644 t
ORIGIN      
        1 atttaatata cctatagggg gtataataaa aatatagatt tctaaataag taatttatca
       61 atattacata tcaaaataaa acgttacaaa ttagctgaaa atatattgaa agaaaggaag
      121 attttattat gagttcaaat aaagtggtag aaagttataa aattacaggt atgacatgtg
      181 cagcttgtgc aaaggcagta gaaagagtaa ctaagaagat ggatggagta tatgaccaaa
      241 gtgtaaatat agctactgaa aaacttaaaa tagaatatga taattctaag gttaattttg
      301 atgatataaa gcaggttgta gaaaaagcag gatatggaat tataaaggaa gaaagtaata
      361 aaaaaattga tatgaaaatt gatggaatga catgtgcagc ttgtgcaaaa gctgtagaga
      421 gagtggtaaa aaaattggat ggagtagaaa gtattagtgt gaatattgca acagataaag
      481 ctaatataga ctatgaccca tcaaaagtaa aattatctca aataaaagca gctattgaaa
      541 aagctggata taaaccaata gaagaggtaa gaaataaggt tgatgtggat gaagataaat
      601 tgagaaaaga aagagaaatg aagtctttat ttgttaaatt tatagttgca atagtatttg
      661 cagtacctct gttttatata gcaatgggac ctatgataat aaagcctata gggccatggc
      721 ctttgccaga aattataaat cctatgacaa atacttttaa ttatgcatta attcagttaa
      781 ttttagtaat accagtaatg atagctggtt ataaatttta tataaatggg tttaaatcac
      841 ttttttcttt aagccctaat atggattctt tagttgcaat aggaacatta gccgcatttt
      901 tatatagtct atatacgaca ctacaaatag ccaatggaca aattcaaggt atgcaccatc
      961 atcagttgta ttatgaaagt gcaggaataa taatagcact tatcttactt ggtaaatatt
     1021 tagaatcaaa atcaaaagga aaaacatcag aagcaattaa aaagcttatg gggctacaac
     1081 ctaaaactgc aatagtgtta gttgatggta aagaagttga aactcctata gaagaagtag
     1141 agataggaga tatactatta gtaaaaccag gaactaaaat acccgtggat ggagttgtaa
     1201 tagaaggata tacatcagtt gatgaatcaa tgcttacagg tgaaagtata ccagtagaaa
     1261 aaaatgttgg aagtaaggtg acaggagcta gtattaataa aaatggtgtt attaagttta
     1321 aggctgaaaa aattggtgga gatactgctc ttgcccaaat aataaagtta gttgaagatg
     1381 cacaaggtac aaaagcacct attgctaaac ttgccgatac tgtatctgga tattttgtac
     1441 caatagtaat tgcaatagct gtagttgcat cattattgtg gtttttaata ggaggaaaag
     1501 atatagtatt tgtacttact atatttatat cagtacttgt aattgcttgt ccttgtgcat
     1561 taggattagc aacaccaact gctataatgg ttgggactgg aaagggcgca gaaaatggaa
     1621 tcttgataaa aggtggagag gcactagaat ctgcacataa agtaaataca gtgatatttg
     1681 ataagacagg tactataaca gaaggaaagc caaaagtaac agatatagta ttaaataata
     1741 atgtaaaaga agaatatctt atcaagattg cttcaagtgc agaaaaaggt tcagagcatc
     1801 ctcttggaga agcaatagtt aagtatggag aagagaagaa tataaaattt gaaaaagttg
     1861 ataattttaa agccatacca ggtgctggta tacaagttac tattaatgat gaaagtatat
     1921 tacttggaaa tagaaagctt atgaatgaca ataatataaa gcttggggat ttagaagaaa
     1981 aatcaaatgt attggcaagc caaggtaaaa cacctatgta tatagctgta gatggaaatt
     2041 tatcaggtat aatagcagtt gcagatgtag tcaaagaaag tagtaaaaaa gcaatagaaa
     2101 tacttcatga tatgggaata aaagttgcaa tggtaactgg agataatgcg aaaactgcaa
     2161 atgctatagc aaatcaagta ggtatagata tggttttggc agaggtttta ccagaagata
     2221 agtcaaaaga agttgaaaaa ctacaaaatc aaggaaagtt tgttgcaatg gtaggggatg
     2281 gaataaatga tgccccagct cttgcaaaag cagatatagg gatagcaata ggaagtggaa
     2341 cggatgtagc cattgaatct gcggatatag tacttatgaa gagtgattta attgatgttc
     2401 caacagcaat taaacttagt catgaaacta taaaaaatat aaaacaaaac ctattttggg
     2461 catttggata taatacgatt ggtatacctg tagcagcagg aatattatat gtatttggag
     2521 gacctttatt aaacccaatg attgcagcag cagcaatgag cttaagttca gtttcagtcg
     2581 tatcaaatgc acttagactt aaaaacttca aggcatataa gagagattag attaataaat
     2641 taaataaaat gtatccaaaa gagctatctt taaattaaaa ggattaattt acaaattaag
     2701 atagctcttt tttcatgtaa aattcacaac aataagataa aatataagta atatacgaaa
     2761 ttttaaataa aatagaaagg attgatagaa tgaattcgtc aatacttgta attgaagatg
     2821 attcaaatat acaagagtta atatcagaat ttttaagtgc agaaggttat caagttgata
     2881 cagcaaatga tgggttggaa ggtatacaaa agtttaagca aggaagttat gatttagtaa
     2941 tattagatat aatgatgcca aacttagatg gttatggagt ttgtaaaatg ataagaaagt
     3001 catcaagcgt tccaattata tttttaactg ctttaaatga tgaaggagac caacttaaag
     3061 ggtttgactt agaatgtgat gattatataa caaaaccatt ttcatttaat cttcttataa
     3121 aaagagtaga agcaatttta aggcgaagta acaagactat aaatgacaaa tttatagttt
     3181 ttgaaaaatt aaaattagat ttaaacacat atattgcaga gatagatgga gaacctatag
     3241 aacttacact aaaagagttt aatatattaa aagctttgat agaaaagtat ccacaagtta
     3301 taactaggga aggtcttttg gatagtatat ggggttatga ctattatgga gatactagga
     3361 ttgttgatgc acatataaaa aatataagaa aaaaaatatc gttaccatat attaaaactg
     3421 taaaaggaat aggatataca cttgagaagg atatttgata agtgggaaaa gttaagtata
     3481 aagtataagt tattttcaat aacgacatca ttattaatag cgttagcttt aataatatat
     3541 ctaattttat attttttatt gccatcatat tatcatgagt ataaaattga gtctcttcaa
     3601 gaaagtttaa aatctttagt agatagttca attcatttcg atacatatac tttagaagaa
     3661 agactttatt atatggctaa agaccaaaat ttggctattt tattaaaaga taaccaaggg
     3721 aaaattgtat atggtaagaa tgaagtagtt attttaagat atagtaagta catgattaat
     3781 agtcttgagg atgagtatag aacttctata cctatatata ctaaagatgc aaaagatggt
     3841 ccatatactt tagaactcgt tatgccatta caaccaattg acgaagcaaa cgaagttata
     3901 agaaaactaa tgccatacat catatcaata gcaatattga tagcgattat tggagcatat
     3961 atatattcaa ttgtaataac aaagcctctt ataaatataa tagagagtga gagagaacag
     4021 gaatatagaa ggaaggattt tgttgctaca atatcacatg aactaaaaac tccaataacc
     4081 ataataagtg gtcaaattga aggtatgatt tatagtgtag gtaagtataa ggatagggat
     4141 acttatctga aaaaatcata tgaatgtaca caagaattaa aagatttagt aaatgaaatg
     4201 atagaggttt caaaatcaga aatacttgaa aaagatttga aattggtatc aataaatata
     4261 agtgaattat taaatagact tgtaaagaga caagtatttt taatagaaga aaaacatatg
     4321 aaaactattt taaaaataga agaaaatttg gaaattaaag cagaccaaga gagaataact
     4381 aaggcaatca ataatataat aaataatgcc ataaaatatt ctcctgaaaa atcagagctt
     4441 ataattagac tctatgataa aaataaacga ataagtaaaa agacttctaa tcaaagagtg
     4501 gttttagaaa ttgaaaatac tggtgtaact atcgagaaac gttatttaga agagatattt
     4561 aatccatttt atagaataga aaaatcccgt tcaagaaaaa ctggaggaag tggacttgga
     4621 ctttatatag tcagtcaaat ttttaaaagt catgggtttg attatagcat aaaaaacaaa
     4681 gaaaactctg tagtattcac agtagaattt aaaaattaat tacttattat aaagaatata
     4741 taactttcaa taatagttca ttttaaaatc acaaaaaatt cataatgaaa ctgtattata
     4801 taaatataaa gaagcttaaa accataaaat acaccaacta taatgatgag atttccttac
     4861 atcctccata taagtcatca ttatagtaac ccaacaatta agttactatg aatttaaaaa
     4921 ataacaccga gcatataatc tatgcttagt gttattttta ttttgaaata aaaaatattt
     4981 aatttattga aatgtataga tttagacagt ttaaactata tttaagactt aaaaattata
     5041 attgatatta caaataaata tatgtacttt gggaaaaata aatataggaa gtattatatt
     5101 tacagaggag gtagttttga tggatagatt aaaaagagat atgaaaaata tgaaagaaga
     5161 tttaaaagaa ggtgttaaaa agacagctta tgttatgaaa aatacagctg aagatttgaa
     5221 agatgatgca caaggtgcta tggtaaactt agaaatgaaa aaagatgaga tgatggaaag
     5281 atatgaacaa aagaaatttt ctaaagaatt agagaagcaa atgaagaatg aaatgaaata
     5341 agcttttatt tatgaataac aaattattgt attcatataa aaagtagcca gtaatattaa
     5401 atagtttatt taaggaaata gactttctaa aaataactgt attagattta gaaagtctat
     5461 ttcaaattag ttttataaat agacataag
//
LOCUS       DAMJWP010000228         5356 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.235, whole genome shotgun sequence.
ACCESSION   DAMJWP010000228 DAMJWP010000000
VERSION     DAMJWP010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5356
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.235"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(234..2513)
                     /locus_tag="PC700_003189"
     CDS             complement(234..2513)
                     /locus_tag="PC700_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2582459.1"
                     /translation="MLSDAKNNRYGLSIIAVISILVITISCLIYGSRLNKTLGEETNQ
                     YLSEIANQSVNVLKKQINGDIKLLESISIFIEGEESFEVDNILSILKRQAINSSFKRM
                     GVILPNGTAYTTDGYIEDFSKRDYFLKSMQGEVVITEKLKDVIEDDKNTNSNINVYSV
                     PIYKDNEVKGVIFATHSTKLYEKILSVPTFNGKGYSYIARSNGNIVLNPSSKNANSTK
                     ENLFSYIGRSDSISKSSINNMKSNIKNFKSGSLSYSMFGKGYYLSYAPIGINDWYLFS
                     EVPKTAISEKSYAIIKLTLSACVVLIIIFTALTIYILFIHKKSKEKLEEFAFKDNVTG
                     IGNSNKFNLEGGKFLSSHEKKNLVLIYFDIDKFKLVNDRFGYEEGDRVLKEIAEIIKN
                     IFKEQSVFSRISNDNFAIIFEKKNNRESIIEICELIRKKLSMIKTSLGVELNLIPSIG
                     VYFVEEGETNISTCLDKAMIAKTTVKRKYLKVYEIYAENLKETLIEERDIEQEMHDAL
                     KNEQFKVYLQPKVDLSTTKIVGAEALVRWQHPKKGLISPGVFIPIFEKNGFITELDMF
                     VFAQVCKNFKRWENENFPTFPISINLSRVHLENPGFISELERITKEYEIEPNLIEIEL
                     TESAIFDNTKILFKIMQTLKSVGFKISMDDFGSGYSSLNMLKDMPIDVLKLDRQFFIT
                     VGNAKKSQIVVSSIVQMAKQLDIKVVSEGVETVEQAEFLRFIGCDMAQGFLFARPMPI
                     EEYEKLILDRYSLNRKETN"
     gene            complement(2784..4238)
                     /locus_tag="PC700_003190"
     CDS             complement(2784..4238)
                     /locus_tag="PC700_003190"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HDF2582460.1"
                     /translation="MKLNDIIQGLDIINVKGELNIDINNVQYNSRKVTKGTLFICIKG
                     FVSDGHKYIKDAIEKGASAFLVEEDVEIKGCTFIKVKDTRKDMAKVADNFYNHPSQKF
                     NVIGVTGTNGKTSITTILNEILTLNKNKVGLIGTIKIFDGEKDIVSNSTTPESIDLQY
                     HFNNMLDNGCDYCAMEVSSHSLALNRVDETDFKLGIFTNLTPDHLDFHKDLEDYRKAK
                     EKLFFKTTMANIINIDDEGGKKIYENIKGINVPCYTYGVDTKADFMARDIKSDSDGVS
                     YRLITPSYEEVIFIPVPGMFTVYNTLAVIAACYVLGIPKPIYKEGLRLSNGVSGRFET
                     VPNDKGISVIVDYAHTPDALENVLKTTQQFAEGKIISVFGCGGDRDTEKRPLMGAIGQ
                     KYSDLCIITSDNPRTEEPEAIIKDILEGIDKKKENYHVVVDREQAIAEAISMAKKDDV
                     VIITGKGHETYQIIGKVKHHFDDKEVANECLSKM"
     gene            4402..5271
                     /locus_tag="PC700_003191"
     CDS             4402..5271
                     /locus_tag="PC700_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2582461.1"
                     /translation="MDNKIGYLNDNFKIFNIRDKKDIQFEYHNHDFNKIIIFINGNVT
                     YFVDGIPYKLKPWDILFISNNEIHKPVIDSNVFYERIAIWISPEFMKKNSDATSDLEK
                     CFKIATENKCNRLRLNIHDIESIKSFIEQINISESNTEFGNEILRNALFIQLMIFLNR
                     IFLKNENMESTEDVFYDETIKKVLNYINSNIDDKLSIDSISSEFFISKYYLMRKFKSH
                     TGTSIHNYIVQKRLILAKSLITNGYLMSEVCNKCGFNDYSSFVRAFKKNYGVSPKNYI
                     NNKNPLDITLLDE"
BASE COUNT         1829 a          836 c          564 g         2127 t
ORIGIN      
        1 attactatta ttactatcta aagtattttg ctataatccc atatactctt acctcaaaaa
       61 cattcaaatg gaaaaaatac tacatattaa taaactttgc ccaataattc ctaaaataat
      121 atcctaacaa tgatttatgt ttttataata gtatattttt acataaaaaa gtgaacattg
      181 attaacaaag ttcacttttt tataaatttt tatgttataa ttcacaattt tatttaattt
      241 gtttcttttc tatttaaact atatctatct aatattaatt tttcatattc ttctattggc
      301 attggtctag caaataaaaa tccttgggcc atatcacaac ctataaacct taaaaactct
      361 gcttgctcta cagtttcaac accttctgaa acaactttta tatcaagttg ctttgccatt
      421 tgaacaatac ttgaaactac aatttgactc ttttttgcat ttcccactgt tataaagaat
      481 tgcctatcta atttcaatac atcaattggc atatctttaa gcatatttaa agaagaataa
      541 ccagaaccaa aatcatccat agaaatttta aatccaacag atttcaaagt ttgcattatt
      601 ttaaataata tttttgtatt gtcaaatatt gcactttctg tcaattcaat ctcaattaaa
      661 tttggttcaa tttcatattc ttttgttatt ctttcaagct cagaaataaa acctggattc
      721 tcaagatgta ctcttgataa gtttattgat ataggaaatg tggggaaatt ttcattttcc
      781 caacgtttaa aatttttaca aacttgggca aatacaaaca tatccaattc tgttataaaa
      841 ccatttttct caaaaatagg aataaataca cctggactta ttagaccttt tttgggatgt
      901 tgccaacgta caagcgcttc agcacctaca attttagtag ttgataaatc aaccttaggc
      961 tgtaaataaa ctttaaattg ttcatttttt aaagcatcat gcatttcttg ttcaatatca
     1021 cgttcttcta tgagagtttc ttttaaattt tcagcataaa tctcataaac ttttaaatat
     1081 tttcttttta ctgtagtttt tgcaatcata gctttgtcaa gacaggtaga aatatttgtt
     1141 tctccctctt caacaaaata tacacctatg gatggaatta aattgagttc tactcccaaa
     1201 cttgttttta tcattgacaa ttttttacgt ataagttcac atatttctat aattgactct
     1261 ctattatttt tcttttcaaa tattatagca aaattgtcat ttgaaattct tgaaaataca
     1321 gattgctctt taaatatatt cttaattatc tctgctattt cctttaatac tctatctcct
     1381 tcttcatacc caaacctatc atttactagc ttaaacttat ctatatcaaa ataaattaaa
     1441 actagattct ttttctcatg agaggataaa aattttcctc cttcaagatt aaatttattt
     1501 gaattgccaa tacctgtaac gttatcttta aaagcaaatt cttccaattt ttccttactc
     1561 tttttatgta taaatagtat ataaattgtt aatgccgtaa agattataat caacactaca
     1621 catgctgata atgtaagttt tataatagcg tatgatttct cagatatagc agttttagga
     1681 acttcagaaa aaagatacca atcattgatt cctattgggg cataacttaa ataatatcct
     1741 tttccaaaca tactataact taatgaacct gatttaaagt tttttatatt tgacttcata
     1801 ttattgatac tagattttga tattgaatca cttctgccaa tgtaagaaaa taaattttct
     1861 ttagtagagt tggcgttttt gctgcttgga tttaaaacaa tattaccatt acttctagct
     1921 atataagaat atccttttcc attaaatgta ggtacagaaa gtattttttc atacaacttc
     1981 gtactatgtg tggcaaatat aactccttta acctcattat ctttataaat tggcacagaa
     2041 tatacattaa tattactatt ggtattttta tcatcttcta ttacatcttt taatttttct
     2101 gttataacca cttctccttg cattgatttt aagaaataat ctcttttaga gaagtcttct
     2161 atataaccat ctgtagtgta tgctgtacca tttggtaaaa taactcccat tcttttaaat
     2221 gaactattaa tagcttgtct ttttagtata gaaaggatat tatctacttc aaaagactct
     2281 tctccctcaa taaatattga aattgactct agtaacttta tatctccatt aatttgtttt
     2341 tttaacacat tgacgctttg atttgctatt tctgaaagat attgattcgt ttcttcacct
     2401 aatgtcttat tcaaccttga accataaatc aaacaggaaa ttgtaattac taagatactt
     2461 atgacagcaa ttatggacaa accataccta ttattttttg catcacttaa cattatgctt
     2521 cctgcctttc tcgaatctat gtatgtataa tcattatctt atgacaatta ttgtaaacat
     2581 tttataataa tacttatttt tccatattta ctgtaattat acaaaattat aaactttatt
     2641 acaacaataa attaattaat atagatatta gggtttatat tattgcatta tctattttac
     2701 aatcatttac atataaaaaa atgtaatatc aaagtgaaac ttgatattac atttttgttc
     2761 ttgtattttg tttaattttt atgctacatt ttacttaaac actcatttgc aacttcttta
     2821 tcatcaaaat gatgcttaac tttacctata atttgataag tttcatgtcc cttacctgtt
     2881 ataattacaa catcatcctt ttttgccata cttatagctt ctgctatagc ttgttctcta
     2941 tcaacaacaa catggtaatt ttcttttttc ttgtcaattc cttcaagtat atcttttatt
     3001 attgcctctg gttcttcagt tcttggatta tcagaagtta taatacataa atcagaatat
     3061 ttttgtccaa ttgctcccat caaaggtctc ttttcagtat ctctatctcc accacatcca
     3121 aacacagaaa ttatttttcc ttctgcaaat tgttgagttg tctttagtac attttcaagt
     3181 gcatcaggag tatgagcata gtctacaatt acacttatac ctttatcatt tggaactgtt
     3241 tcaaaccttc ctgatactcc attagaaagt ctaagacctt ctttatatat tggtttaggt
     3301 atgccaagta catagcaagc agctataact gctaaagtat tatatactgt aaacattcct
     3361 ggaactggta taaatattac ttcttcatat gaaggtgtta ttaatctata agatactcca
     3421 tccgaatctg attttatatc tctagccata aaatcagcct tagtatcaac accataagtg
     3481 taacaaggta catttatacc ttttatattt tcatatatct tttttccacc ttcatcatct
     3541 atgtttatta tatttgccat agtagtctta aaaaataatt tttcttttgc ttttctataa
     3601 tcctctaaat ctttatgaaa atctaaatgg tctggtgtta aatttgtaaa tatgcctagt
     3661 ttaaagtctg tttcatcaac tctatttaat gccaatgaat gtgatgaaac ttccatcgca
     3721 cagtagtcac atccattatc caacatatta ttaaaatgat attgtaaatc tatactttct
     3781 ggagttgttg aattggaaac aatgtctttt tctccgtcaa aaatttttat agttcctata
     3841 agtccaactt tattcttatt taaagttaat atctcattta gtatagtagt tatactagtt
     3901 tttccattag tacctgtaac tcctataacg ttaaatttct gagatggatg attataaaaa
     3961 ttatcagcta cttttgccat atcttttctt gtatctttta cctttataaa tgtacatcct
     4021 tttatttcta catcttcttc aacaagaaat gcacttgctc ccttttctat ggcatctttt
     4081 atatacttat gaccatctga cacaaatcct tttatacata taaataatgt accttttgta
     4141 acctttcttg aattatattg tacattatta atatctatat ttaattcacc cttgacattg
     4201 attatatcca acccctgtat aatatcattt aatttcataa atgctttcct ccctttaaac
     4261 tctttctgct aaaattacac tacatataat attatattaa gaatttttta ctatgtgaat
     4321 ggttaaaaat gcttgaaata ttgcaaaaaa tgctctataa ttttctataa tataactgag
     4381 gaaaggtctt ctataaaaac tatggataat aaaatcgggt acttaaacga taactttaaa
     4441 atatttaata ttagagataa aaaagatata caatttgaat accataatca tgattttaat
     4501 aagataatta tatttataaa tggaaatgta acttattttg ttgatggtat tccctataaa
     4561 ctaaagcctt gggatatact ctttatcagc aataatgaaa tccataaacc agttatagat
     4621 tcaaatgtct tttatgagag aattgcaata tggattagcc cagaatttat gaaaaaaaat
     4681 agtgatgcta ctagtgactt agaaaaatgt tttaaaatag ctacggagaa taaatgcaat
     4741 agattgcgtt taaatataca tgatattgaa tctattaaga gttttattga gcaaataaat
     4801 atttccgaat ccaatactga atttggaaat gaaatattga gaaatgcatt atttatacaa
     4861 cttatgatat tcttaaacag aattttccta aaaaatgaaa atatggaatc aacagaagac
     4921 gttttttatg atgaaactat taagaaagta ttaaactata taaattcaaa tatagatgat
     4981 aagctttcaa tagatagcat atcgtctgaa ttttttataa gtaagtatta tcttatgaga
     5041 aaatttaaat ctcatactgg aacgtctata cacaattata tagttcaaaa aagattaatt
     5101 ttagcaaagt cactaataac caatggttac cttatgagtg aagtttgtaa taaatgtgga
     5161 ttcaacgatt attctagttt tgtaagggca tttaaaaaaa actacggtgt atctcctaaa
     5221 aattatataa acaataaaaa tcctttagat attactcttt tagatgaata gataatttat
     5281 gtaaatttta ttttaataaa aacttgaagt attagtgtat ttatcttact aatacctcaa
     5341 gttttttaat gtttta
//
LOCUS       DAMJWP010000229         5349 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.236, whole genome shotgun sequence.
ACCESSION   DAMJWP010000229 DAMJWP010000000
VERSION     DAMJWP010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5349
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.236"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(103..2322)
                     /locus_tag="PC700_003192"
     CDS             complement(103..2322)
                     /locus_tag="PC700_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2582462.1"
                     /translation="MTGRNKSFTLITILFLTSLFLTMGYIYIEDTKHLLMSEAKIHIK
                     EVAVQGSQLAQRQIEKDLDVLNIFSNYYALNQDIPNEEKMKNLLDEMENQKFYTMAIV
                     DINGNAENTKGDKFSVKDREFFKNSIKGKKYVSSPYVDEVNKSIKKITISVPLLNNDK
                     VVGVLYCTYNINTLMKLINVSFYENNSISYVVRNDGTIILHPQEDSLSKNIYKLLKQD
                     NDIREINRLKKELQENKTGATVLNMLEERRYLGYATMDNGNSENNYIKDWNLIFSIPE
                     TVVFSNSEQIINRAIYAVLFIILIFIAIIFYIIYIKKSNEKEILSLAYEDKVTHIGNQ
                     NKFYRECSKYLLDKPSLNYIIVYFDINNFKMINDTFGYEFGDNLLITIAKALKEELTE
                     GEVYARLSSDYFAIFCDYKNGKNKRIRKIDSIRNNIESNLSIVFEISLCVGIYFVEEG
                     EVDIQKAVNKANMARSVAKGKNINYAIYNEDVRNKLSEESMILDDIKIALVKNQFEVY
                     YQPKFSLVTGEMIGSEALIRWNHPEHGFISPAVFIPIAEKSKLILKIGRFVFERVCND
                     LSEWKKQGKKIVPVSINLSRVELYQPDIVKFINKTIQMYNLSSDLIEIEITETVAINE
                     LNILKNVLNELRKYGFSISMDDFGTGYSSISCLRDMPIDILKLDKSFLNGIEHDERSR
                     NIAKSIVSLAKSLDLVVIIEGVESKEQAELMKQFGCDLVQGFYFARPMPAKNFLDLL"
     gene            complement(2598..5252)
                     /gene="adhE"
                     /locus_tag="PC700_003193"
                     /gene_synonym="adhC"
     CDS             complement(2598..5252)
                     /gene="adhE"
                     /locus_tag="PC700_003193"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDF2582463.1"
                     /translation="MAHKEQVIIENKKENEVMSVGLDTVNNVESLVKKLAKIREAQKI
                     FATYTQEQVDKIFLAASLAANKQRVPLAIMAQKETGMGIAEDKVIKNHYASEYIYNAY
                     KETKTCGVIEKDEAFGMTKIAEPIGVIAAVVPTTNPTSTAIFKALLALKTRNGIIFSP
                     HPRAKNSTIEAAKVVLEAAVLAGAPEGIIGWIDEPSLELTTTVMKEVDLTLATGGPGM
                     VKSAYSSGKPAIGVGAGNTPAIIDESADIKTAVNSILVSKTFDNGMICASEQSVIVLE
                     NIYNEVKKEFKERGAYLLDKDETEKIRNIILVNGGLNSKIVGQTACTIAKLAGFEVPV
                     DTKVLIGEVESVEIEEAFAHEKLSPVLAMYKASDFDNAVRKAEKLVEDGGFGHTSSLY
                     IDDVNQREKLDKFTSAMKTCRILINTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVS
                     ENVGVKHLLNIKTVAERRENMLWFRSPGKVYFKKGCLGVALKELKDVMNKKRAFIVTD
                     TFLYNNGYTKAVTDLLDEMNIKHTTFFEVEPDPTLECAKIGAKAMREFNPDVIISIGG
                     GSAMDAGKIMWTLYEHPDVDFQDLAMRFMDIRKRVYTFPKMGEKANFVAIPTSAGTGS
                     EVTPFAVITDQDTGVKYPLADYELMPNMAIVDSDMMMNMPKSLTSASGIDALTHALEA
                     YVSMLATDYTNGLALQAIKSIFEYLPRAYDNGAKDPEAREKMANASTMAGMAFANAFL
                     GVCHSMAHKLGAFHHVPHGVANALLITEVMKFNSSDAPKKMGAFSQYKYPEALKRYAE
                     IASFLGLKGNSDEEKFQSLLVAIEDLKLKVGIPKSIKEFGVEEPKFMDSIDEMVIQAF
                     DDQCTGANPRYPLMNEIKDMYLNSYYGR"
BASE COUNT         1735 a          960 c          576 g         2078 t
ORIGIN      
        1 taaacaatac attgataaaa taaaaaaaca ctaattattt agcagtgatt tattaaaatc
       61 aaacattcat aatagtgctt tatataaaaa ttaaattttg ttttaaagta aatctaaaaa
      121 attcttagct ggcattggtc gtgcaaaata aaatccttga actaaatcac atccaaattg
      181 tttcattaac tcagcttgtt ctttactctc aacaccttct ataattacaa ctaaatctag
      241 tgattttgct agagacacta ttgattttgc tatattgcga cttctctcat catgttcaat
      301 accattaaga aaagacttat ctagtttcaa tatatcaatt ggcatatctc ttaaacaact
      361 aatagaagaa tatcctgtac caaagtcatc catagaaata gaaaatccat acttcctcaa
      421 ctcatttaaa acattcttta aaatatttaa ctcattaata gccacagttt ctgttatctc
      481 tatttcaatt aaatctgaac ttagattata catttgtatt gttttattga taaattttac
      541 aatatctggt tgatacaact caactctaga taaatttatt gaaactggaa ctatcttttt
      601 accttgcttt ttccattcag ataaatcatt gcaaaccctt tcaaatacaa atctcccaat
      661 ttttaaaatc aacttacttt tctctgctat tggaataaat actgcgggac ttataaaacc
      721 atgttctgga tgattccatc ttattagtgc ttcactccct atcatttctc cagttacaag
      781 tgaaaactta ggttgataat acacttcaaa ttgatttttt accaatgcta tttttatatc
      841 atctaaaatc attgattctt cactaagctt attcctaaca tcttcattat aaatagcata
      901 atttatattt ttacctttag caacagacct agccatattt gctttattta cagccttttg
      961 gatatctact tcaccttctt caacaaaata aatacccaca cacagagaaa tttcaaatac
     1021 tatactcaaa ttactttcaa tattatttcg tatactatca atttttctta tacgtttatt
     1081 ttttccattt ttataatcac agaaaattgc aaaataatca cttgaaagtc gtgcatatac
     1141 ttcaccttct gttaattctt cttttaaagc ttttgctatc gtaattaata aattgtcacc
     1201 aaattcatat ccaaaagtat cattaatcat cttaaagtta ttaatatcaa aatatactat
     1261 aatataattt aaagatggtt tatctaaaag atacttacta cactctctat aaaatttatt
     1321 ttgatttcct atatgggtaa ctttatcttc ataagccaaa cttaatattt ctttttcatt
     1381 ggattttttt atataaatta tataaaaaat aattgcaata aatattaata taataaataa
     1441 cactgcatat atagctctgt tgattatttg ttctgaatta ctaaatacaa cagtttctgg
     1501 aattgaaaaa atcaaattcc aatcttttat ataattattt tcactattcc cattatccat
     1561 agtagcatat cctaaatatc ttctttcttc caacatattt aatacagttg cacctgtttt
     1621 attttcttgc aattcttttt ttaatctatt tatttctcga atatcattat cctgttttaa
     1681 caatttataa atattttttg ataggctatc ctcttgcgga tgaaggataa ttgtaccatc
     1741 atttcttaca acataagata tactattatt ttcataaaaa gatacattaa taagtttcat
     1801 cagagtattg atattgtaag tacaatataa aactccaact actttatcat tatttaataa
     1861 tggtactgaa atagtaatct tctttataga cttatttact tcatcaacat atggacttga
     1921 aacatacttc ttacctttaa ttgagttttt gaaaaattct ctatctttca ctgaaaactt
     1981 atcacctttg gtattttcag cattaccatt tatatcaact attgccatag tgtaaaattt
     2041 ttgattttcc atttcatcta aaagattttt cattttttcc tcattaggta tatcttgatt
     2101 taaagcataa tagttagaaa aaatatttaa aacatctaaa tctttttcaa tttgtctttg
     2161 tgctagttga gagccttgta ctgcaacttc ttttatatgt atttttgcct ctgacataag
     2221 aagatgtttt gtatcttcta tatagatata ccccattgtc aaaaataatg atgttaaaaa
     2281 aagaattgta attaatgtaa aacttttatt tctccctgtc attcgactcc tcccctaatt
     2341 agtcaatact tcatatagat aatatatcct ttttataccg attcatcatt tatattgtat
     2401 ataactattc ttaaatgtta cacataaaat caacctaata accataaaaa cattctttta
     2461 ttatcataat aacactattt agttgaattt taaattaaag aataaaaact atcaattttt
     2521 ctaaacaaaa aaactcttat acaataaaac tttgttttat gtataagagt ttttatttta
     2581 tctattttat agtaaattta tcttccataa tatgaattta aatacatatc ttttatttca
     2641 ttcataagtg gatatcttgg atttgctcct gtacattggt catcaaatgc ttgaatcacc
     2701 atttcatcta ttgaatccat aaacttaggt tcttcaactc caaactcttt tatagattta
     2761 ggaataccaa ctttaagttt taaatcttca atagctacca ataaactttg gaatttctct
     2821 tcatcagaat ttccctttaa tcctaaaaat gaagctattt cagcatatct ttttaatgct
     2881 tctggatatt tatattgaga gaatgctccc attttctttg gtgcatctga agaattaaat
     2941 ttcataactt ctgttatcaa aagagcattt gcaacaccat gtggtacatg gtggaaagca
     3001 cctaatttat gagccataga gtgacaaaca cccaaaaatg catttgcaaa agccatacct
     3061 gccattgtag aagcatttgc cattttttct ctagcttctg ggtcttttgc accattatca
     3121 tatgcacgtg gaagatattc aaatatactc ttaatggctt gtaaagctaa tccatttgta
     3181 taatcagttg caagcataga tacatatgct tctaatgcat gagttaatgc atctatacca
     3241 gaagcagatg ttaagctttt tggcatattc atcatcatat cagaatcaac tatagccata
     3301 tttggcatca gttcataatc tgctaatgga tattttaccc ctgtatcttg gtcagttata
     3361 acagcaaatg gtgttacttc tgaaccagta cctgctgatg ttggtatagc tacaaaatta
     3421 gctttttcac ccattttagg gaatgtatat accctttttc ttatatccat aaatctcata
     3481 gctaggtctt ggaaatctac atctggatgc tcatacaatg tccacattat ctttcctgca
     3541 tccattgcac ttcctccacc tatagatatt attacatctg gattaaattc tctcatagct
     3601 ttagcgccta tcttagcaca ttccaaagtt gggtctggtt ctacttcaaa gaatgtagtg
     3661 tgcttaatat tcatttcatc taataaatca gtaactgctt tagtatatcc attattgtaa
     3721 aggaatgtat ctgttacaat aaaagctctc ttcttattca ttacatcttt aagttctttt
     3781 aatgctaccc ctaaacaacc ttttttgaaa taaacttttc ctggtgatct aaaccaaagc
     3841 atattttctc tcctctcagc tactgtttta atattaagta aatgcttaac tcctacattt
     3901 tcagatactg aatttccacc ccaagaccca catcctaaag taagagaagg agctagttta
     3961 aaattatata agtcccctat accaccttga gaagaaggag tattaattaa aattctacaa
     4021 gttttcatgg ctgaagtaaa cttatcaagt ttttctcttt gatttacatc atcaatatat
     4081 aatgaagatg tatgcccaaa tccaccatct tctactaatt tttctgcttt tctaactgca
     4141 ttatcaaaat cactggcctt gtacattgca agtactggtg aaagtttctc atgagcaaaa
     4201 gcttcttcta tttcaactga ttctacttca cctattaaaa ccttagtatc tactgggact
     4261 tcaaagcctg caagttttgc tatagtacaa gctgtttgcc caactatttt agaatttaat
     4321 cctccattta ctaaaattat atttcttatt ttttctgttt catctttatc taataaataa
     4381 gcaccacgct ctttaaattc ttttttgact tcattgtata tattttctaa tactataacc
     4441 gattgttcag aagcacatat cataccattg tcaaaagtct tagatacaag aattgagttt
     4501 acagcagttt ttatatctgc actctcatca attattgctg gagtattacc tgcaccaact
     4561 ccaatagctg gttttccaga tgaatatgct gatttaacca ttccaggacc tcctgtagca
     4621 agggtcaaat ccacttcttt cataacagtt gtagttaatt ctaaagatgg ctcatctatc
     4681 catcctatta tgccttcagg agctccagct aaaacagcag cctcaagaac aacttttgca
     4741 gcctctatag tagaattttt tgctcttgga tgcggagaga aaattattcc atttctagtt
     4801 tttaaagcta aaagtgcttt aaagatagca gtagaagttg gattagttgt aggtacaact
     4861 gctgcaataa cacctattgg ctcagcaatt ttagtcatac caaatgcttc gtctttctct
     4921 ataacaccac aagttttagt ttctttatat gcattatata tatactctga ggcataatga
     4981 ttctttataa ccttatcttc agcaattccc ataccagttt ccttttgtgc cattatagct
     5041 agaggaactc tttgcttatt agctgccaaa gatgctgcta aaaaaatttt atctacctgc
     5101 tcttgagtat atgtagcaaa tattttttga gcttctctga tttttgctag ttttttaact
     5161 aagctttcaa cgttatttac agtatctaaa ccaactgaca taacttcatt ttctttttta
     5221 ttttctatta ttacttgttc tttatgagcc attttaacaa cctcctgaga tagtttttta
     5281 tttattatgt taatttatta acattatagc atttactttt tctattttca agttattttt
     5341 ttaacattt
//
LOCUS       DAMJWP010000230         5347 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.237, whole genome shotgun sequence.
ACCESSION   DAMJWP010000230 DAMJWP010000000
VERSION     DAMJWP010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5347
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.237"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(204..1388)
                     /locus_tag="PC700_003194"
     CDS             complement(204..1388)
                     /locus_tag="PC700_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2582464.1"
                     /translation="MLSKRLNFITPSYTIGISSKVKEMESNGVKVINLSIGEPDFNVP
                     NNAKSYGIDSLNKDYTKYDLVPGLKILREEICKKLIEENNCNYSIDEIVVSSGAKNSI
                     TNTLLALTDEGDEVLLPKPYWVSYPEMIKLVNAVPVFIDTKKENGFKLTKEELEKSIT
                     DKTKILVINNPSNPTGSVYTKDELIEIVDVCIQNKIYILADEIYEKICYTGEFTSIAS
                     LSEEAKDITITINGFSKSAAMTGLRLGYTASNKTIAKAMSSIQGHLISHPSLTAQYIA
                     YGALKDCSIDIDNMVKTYKSRRDLIKSKLDSIDNVGYVNPNGAFYIFIDLSKVSEKFE
                     YKDSFSIEFCNQFLEEYNVAVVPGIAFGMDKYIRISYACSENTFLSGLDKLKEFVYKI
                     MA"
     gene            1761..4112
                     /locus_tag="PC700_003195"
     CDS             1761..4112
                     /locus_tag="PC700_003195"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HDF2582465.1"
                     /translation="MVEFVKKRDGRVIPFNEDRITRAIFLAATNVAEREGIVPDYKLS
                     EQLTQEVIKFLNHKYSESVPSVEDIQDSVVKVLIETGHAKTSEEYIIYRTERSRIRNS
                     KTRLMKAIEEITFEDAEDADIKRENANINGNTAMGTMLQYGSTVSKEFCKTHILKPEH
                     SFAHDNGDIHIHDMDFLNMGTLTCCQIDVKKLFNGGFSTGHGFLREPQDIISYGALAA
                     IAIQSNQNDQHGGQSIPFFDYGLAEGVYKTFKKFYIGNLAKALKLFKGIENNDVIKNI
                     VYNTEKETNQKVGLKRDELYLNLEKEKLIQTFDIDDELVNKMQNFAFEESYRETDKKT
                     YQSMEAFIHNLNTMHSRAGAQVPFSSVNFGTDTSEEGRMVTKNLLLSQERGLGNGETP
                     IFPILIFKVKEGINLNPEDPNYDLFKLSCRVSAKRLFPNFSFLDAPFNAKYYKKGEPD
                     TEATYMGCRTRVLSNVCGSETVSGRGNISFTTVNLPRLGIKHGIINNEKANLDGFFEE
                     LDEKINLIIEQLLERFEVQGNKKMKNFPFLMGQGVWKGSDDLVPEDTLKEVIKQGTLT
                     IGFIGLAECLIALIGKHHGESKEAQELGLKIVSHMRHKMDEATDKYKLNFSLMGTPAE
                     GLSGRFTKIDKKVYGEIKGITDKEYYTNSFHVPVYYNISAYDKIEIEAPYHELTNAGH
                     ITYVELDGDPSDNLEAFETVIKAMKDLGIGYGSINHPVDRDPICGFSGVITSNICPVC
                     GRNEDESDIKFERIRRITGYLVGTVDRFNNAKKAEVRDRVKHR"
     gene            4134..4673
                     /gene="nrdG"
                     /locus_tag="PC700_003196"
     CDS             4134..4673
                     /gene="nrdG"
                     /locus_tag="PC700_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HDF2582466.1"
                     /translation="MKIRMSSTISYDSIVDGPGLRMVIWTQGCIHNCKECHNPQTHDL
                     CGGFYMDTEEIINKVKSLKLQKGITLSGGEPFLQPEPLEEIAREAKINGLDVWSYTGF
                     TFEQLLDKKNRAYFKNLNLLKQIDILVDGKFIAEKKDISLKFRGSSNQRIIDVQKSLK
                     YKKVFLVEQYMKDDLSIAE"
BASE COUNT         2089 a          593 c          850 g         1815 t
ORIGIN      
        1 ttataataaa tttagaattt aacttatata gtttaatttg ataatttgat gaaaaaaata
       61 aaagacttac tgatttttaa atctataggt cttttatttt ttatctaaca tttaaattaa
      121 gttgttgaat atatcttata ttacttactt tatatattaa ttaccattaa tacattatat
      181 ctaattacta ttcatctaaa ttattatgcc attattttat atacaaattc ttttaatttg
      241 tctaatccag ataaaaaagt attttcactg caagcatagg atatacgaat atatttatcc
      301 ataccaaatg ctatgccagg aacaactgca acattatatt cttctaaaaa ttgattacaa
      361 aattctatag aaaaactatc tttatattca aatttctcag atactttaga aagatctata
      421 aatatataga atgcgccatt tggattcaca tatcctacat tatcaataga gtctagtttt
      481 gatttaatta aatctcttct tgatttatat gttttaacca tattgtctat gtcaattgag
      541 caatctttca aggctccata ggctatgtat tgtgctgtta gacttgggtg agatataaga
      601 tgtccttgta tagaactcat tgctttagca atcgttttat tagatgcagt atatcctaat
      661 cttaatccag tcattgcagc tgacttagaa aaaccattta tagttattgt tatatcttta
      721 gcttcttcgc ttaatgatgc aattgatgta aattcaccag tgtagcatat tttttcatat
      781 atttcatctg ctaaaatata tattttattt tgtatgcata catctactat ttctattaat
      841 tcatcttttg tataaacact accagtagga tttgaaggat tgtttattac aagaattttt
      901 gttttatctg ttatagattt ttcaagttct tcttttgtta acttaaatcc attttctttt
      961 ttagtatcaa taaaaactgg aacagcatta actaacttaa tcatttcagg ataactaacc
     1021 caataaggct ttggtaataa gacttcatct ccttcgtctg ttaaagctag taaagtattt
     1081 gttattgagt tttttgctcc acttgataca actatttcgt ctattgaata gttacagtta
     1141 ttttcttcaa taagtttttt acagatttcc tctctaagta tttttaatcc tggaactaaa
     1201 tcatatttag tgtaatcttt atttaaagaa tcaataccat aagattttgc gttattaggt
     1261 acattaaaat cgggttctcc tatactaaga tttattactt taactccatt actttccatt
     1321 tctttaactt tagaactaat acctattgtg taggatggtg ttataaagtt tagtcttttt
     1381 gataacataa aatgcctcct tttaaattat tatttatatt atacgacaaa tttaaagaat
     1441 ttacataagg aaatttgtag atatatacat taatatatat aatagcctac actttttgcc
     1501 taaaattagt gtaaaaaaaa gagaaaaaaa ttttaacttt caaagttgtt agaaacgcta
     1561 ctgttcaaaa tatccacata gtatttgcaa tgatttgcgc aagtttgact attatgtaca
     1621 aatttggtaa tcttaatata gcaattatta agataatccc tttaacttag ttgaagggga
     1681 tttttttaga cttaacatac tacatatagt ggtattggga ttaataaaat acaatatata
     1741 gtaacaggag gtttttttaa ttggttgaat tcgtaaaaaa aagagatgga agagtaattc
     1801 catttaatga agatagaata acaagagcta tatttttagc agcaacgaat gttgcagaaa
     1861 gagaaggaat agtacctgat tataaattat ctgagcaatt aactcaagaa gtaataaagt
     1921 ttttaaatca taaatactca gaatcagttc caagtgttga agatatacaa gattctgtag
     1981 ttaaggttct tatagaaact ggccatgcaa aaactagtga agaatatata atatacagaa
     2041 ctgaaagaag tagaataaga aattcaaaaa caagattgat gaaggcaata gaggaaatta
     2101 cctttgaaga tgcagaagat gctgatataa aaagagaaaa tgcaaatatt aatggtaata
     2161 cagcaatggg aactatgtta caatatggaa gcactgtatc taaagaattt tgtaaaacac
     2221 atatattaaa accagaacat tcttttgctc atgataatgg agatatacac atacatgata
     2281 tggatttttt aaatatggga acattaactt gttgtcaaat agatgttaaa aaactattca
     2341 atggtggatt ttcaacagga catggctttt taagagagcc acaagatata ataagttatg
     2401 gtgcattagc tgcaattgct atacaaagca atcaaaacga tcaacatgga ggtcaaagta
     2461 taccattttt cgattatgga ttggctgaag gtgtatataa gacatttaag aaattctaca
     2521 taggaaattt agctaaagca ttgaagttat ttaaaggaat tgaaaataat gatgtgataa
     2581 agaacatagt atataataca gagaaagaaa caaatcaaaa agttggtctt aagagagatg
     2641 aattgtatct aaacttagaa aaagagaaat taatacagac ttttgatata gatgatgaac
     2701 ttgtaaataa gatgcaaaac tttgcatttg aagaatcgta tagagaaact gataaaaaaa
     2761 catatcaatc tatggaagca tttatacaca atctaaatac aatgcactct agagcaggtg
     2821 cacaggtacc attttcaagt gtaaacttcg gaacagatac ttctgaagaa ggaagaatgg
     2881 taactaagaa tcttctatta tctcaagaaa gaggtttagg taatggagag acaccaattt
     2941 ttccaatttt aatatttaag gtcaaagaag gcataaattt aaatcctgaa gaccctaatt
     3001 atgatttatt taaattatca tgcagagtat cagctaaaag actatttcct aattttagtt
     3061 ttttagatgc gccttttaat gcaaaatatt ataaaaaagg tgaaccagat acagaggcta
     3121 catatatggg ttgtagaaca agagtactta gtaatgtgtg tggttcagaa acagttagtg
     3181 gtagaggaaa catatctttt actacagtaa atttaccaag attgggtata aaacatggta
     3241 ttataaataa tgaaaaggct aatttagatg gtttctttga agaattagat gagaagataa
     3301 acttaattat agaacaatta ttggagcgct ttgaggttca aggaaataaa aagatgaaga
     3361 attttccatt cttaatgggg caaggtgtat ggaaaggttc tgatgattta gtaccagaag
     3421 atacactaaa agaagtaata aaacaaggaa cattgacaat tggatttata ggtttagcag
     3481 agtgccttat tgctcttata ggtaaacatc atggagaaag taaagaagct caagaattag
     3541 gattaaaaat agtgtcacat atgagacata aaatggatga agcaacggac aaatataaat
     3601 taaatttctc cttaatgggt actccagcag aaggtctttc aggtaggttt actaaaatag
     3661 ataaaaaagt ttatggagaa attaaaggta tcacagataa agaatattat actaattcat
     3721 tccatgtacc agtttattac aatataagtg catatgataa aattgagata gaagctccat
     3781 accatgaatt gactaatgca ggtcatataa catatgtaga gttagatgga gatccatctg
     3841 ataatttaga ggcttttgaa actgttataa aagctatgaa agatttgggt ataggatatg
     3901 gaagtataaa tcatcctgta gatagagacc caatatgcgg tttttcaggt gtaataacaa
     3961 gtaatatatg ccctgtatgt ggaagaaatg aagatgaaag tgacatcaaa ttcgaaagaa
     4021 taagaagaat aacaggatac ttagttggta cagttgatag atttaataat gctaaaaaag
     4081 cagaagtaag agatagagta aaacatagat agttttattt gggggaataa gttatgaaaa
     4141 taagaatgtc atctactata agttatgaca gcatagttga tggaccagga ttaagaatgg
     4201 taatatggac tcaaggatgt attcataatt gtaaagagtg ccataatcct cagacacatg
     4261 atttatgtgg aggattttat atggatacag aagaaattat aaataaagtc aaatcattga
     4321 aattacaaaa aggcattacg ctatcaggag gagaaccatt tttacaacca gaaccattag
     4381 aagaaatagc aagagaagct aaaataaatg ggttagatgt atggtcatat actggattta
     4441 cgtttgaaca actgttagat aaaaaaaata gagcgtattt taaaaattta aatttactaa
     4501 agcaaattga tattttagtg gatggaaaat ttatagctga aaaaaaagat ataagcttaa
     4561 agtttagagg gtcatcaaat caaaggataa ttgatgttca aaaaagtttg aaatataaaa
     4621 aagtgttttt ggttgagcaa tatatgaagg atgatttatc tatagcagaa taaaatataa
     4681 ataaacagga ttaaatatat gcatatattt aatcctgttt attttcatat aatatttcaa
     4741 gtttgtataa tgtaacaatt aaaccaaaag actcattgta tatttgaaat taagtttttt
     4801 atagtttata ggtgttttat gtggattacg aatgatatag tatcgataca ttatttaatc
     4861 gattcttatt ccaagaaaca attaatttat agaaattctt aattgttaca atataaatat
     4921 ctagataagt tataagatac tgataaataa tttataaagt ttattaatct aatattgtgt
     4981 gtgaatgtta agtggtcaag tagtctaaaa aatgaaaaag atttaaaaaa gtgttttaac
     5041 taaacacata agtgttaaat tcatgtataa gtataggtta gatacataag aaataattat
     5101 gtaatatcta tctttgtaaa aaaatattaa attttgtaat ttgtggataa atatgtttat
     5161 aatgtggata atatttaatg taataagtta aagcaatatg aatcaactgt ttataatgtg
     5221 gataatatta taaatgtatg aataattgca gtatttaaaa acaaaaaaag ttaaaaaagt
     5281 tgttgactct aaaagaaaaa gttggtatag taatacttgt cctcaagaaa agggcaaaca
     5341 aataaag
//
LOCUS       DAMJWP010000231         5329 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.238, whole genome shotgun sequence.
ACCESSION   DAMJWP010000231 DAMJWP010000000
VERSION     DAMJWP010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5329
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.238"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(476..1882)
                     /locus_tag="PC700_003197"
     CDS             complement(476..1882)
                     /locus_tag="PC700_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2582467.1"
                     /translation="MDSVKYILRRFIGSSILISLLFIVLNIAGFLLFSYMLFVSKYSD
                     NLDTSDSREIKSNLIMIEDNLILKDGKFILDDSSKEVLHKNKVWAMLIDNNGDRIWGF
                     DLPKEIPSHYTLTDVAKFSRFFLKDYPVYVWEHPNGILVTGYPKDKYTKFNLTYTIKE
                     IEQFPMLIIGMFLVNAIVTFLIALFIGLKMVKSIKPIITGIKLMSKGEPVLLQEKGIL
                     SDISKSINTVSKELQMKDEKLRKKEEARSNWIAGISHDIRTPLSMIIGYAGELEESSS
                     LSNREQEQVSIICNQGVKIRELVNDLNLVSKLEYNMQALNCDKIRVSAFLRELISEFI
                     NNNLDNDFYVELDILNEDIIIDADKKLLKRAISNLIQNSITHNQQGCNIRVTSKSDLD
                     FCYIIVEDDGKGMSQERINSLLKSQNGLSEAYAKGQNHGLGILIVAGIINAHRGKLDI
                     MSIDSQGLKTILKLPLIK"
     gene            complement(1927..2634)
                     /locus_tag="PC700_003198"
     CDS             complement(1927..2634)
                     /locus_tag="PC700_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2582468.1"
                     /translation="MESIKDKKILVVDDHKELLKMIDEILRKEGFSRVFLASSYEEAV
                     RVFRNVKPDCAILDVVLPDGDGFSIMRKIRETSKIPVIFLSARGEDEDRLIGLGLGAD
                     DYIVKPFLPKELTLRLVGILNRVYVPIEEEELPVFKLGDSVVVNLNSACVEKDGQEIS
                     LTAKEHSLLLKLYENKGRIVTNDSLCQAIWGDDMYGYENTLMVHVRRVREKIEKTPST
                     PKHLITVRGLGYKLMIN"
     gene            2796..3719
                     /locus_tag="PC700_003199"
     CDS             2796..3719
                     /locus_tag="PC700_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2582469.1"
                     /translation="MKHMEMIIRTQRLSKEYNKIFRVKDIDLRVPQGAVYGFLGPNGA
                     GKSTTLKMLLGLAKPTQGNINILGKELNEKNRISILKDIGSLIESPSYYGHLTGLENM
                     TIMQRLLDLPKKNVNEALKIVRLENQKNKKVNQYSLGMKQRLGIAMAIMKFPKLLILD
                     EPTNGLDPAGIEEIRELIKSLPEKYGMTVLISSHLLSEIDQIATSIGIINHGELVFQD
                     SIKELHNRGQSQIAIKAKNVVSAQKLLNTKGYMSLIQNGYLTIENLKDSDVAKINGML
                     VHSNIDVFRIEEHKKSLEDIFLELTGKAVTL"
     gene            3719..4480
                     /locus_tag="PC700_003200"
     CDS             3719..4480
                     /locus_tag="PC700_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2582470.1"
                     /translation="MKYLGLEFYKLKRKKIILMTFLFVAVEIMWCIVNSNRAITRNPD
                     MLGGFEYSYMLMSFASLNGLFFPILISIITSRISDIEHKGDTWKLLKSSVTSLNSIYL
                     SKFLCSAILVSIPILMQVLSIVLFGHFRGVIESISVSLLLKYTLGTILVSIAIIALHL
                     WIATVIPNQMIAITFGMIGSFIGLTSGLFYQGIRRLFIWSYYLELSPLSYAYDNVLGS
                     SVYRIDMNFSITILVFLIGMLIYYIGRNHLLKKEV"
     gene            4492..5229
                     /locus_tag="PC700_003201"
     CDS             4492..5229
                     /locus_tag="PC700_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2582471.1"
                     /translation="MLRKSVLGEILKLKNSFIWYVLLALPLISVLIGSGNFYLNQGIL
                     KKEWYSLWTQVSLFYGEFFFPILIAIFCAYICRLEHMNHNWNNVMTLPVNLKNIFLSK
                     LTVVSILTAITQVFFVIFYIVAGNMFGFSGSVPKELTGWIAKGWIISISIASIQLYMS
                     IKIRSFATPIGISLCLSLFGMGLIVAKPAVGIFFPYSMLGSAMGVIKQSALNVNDTIV
                     TICVAFITTIVFSEIANRNFRKNDVIS"
BASE COUNT         1836 a          822 c          689 g         1982 t
ORIGIN      
        1 taaaacattt atcgtacttt aaataaataa ttataataaa atatacttac aatagttaca
       61 tagaagctat tataactaca cagaacaacg tataattaaa acttttttag gatgtcatta
      121 tattttttct taaaattaaa aaacactata ccataattct attttagtag aatttttttg
      181 tcaataataa aattaaatat taactaaaaa ataaaaaaaa tggaattatc taaagaattt
      241 aataacctta caataattcc aaaatattat ttctatgaaa attttgatac catgagtaac
      301 tcagccataa gcgcataaag cacaagttac tctttacttt aataaaactt gacaagaaaa
      361 gaatttatcc ttattctact ctaatagagt tattttgtca ataaagaata aaattatttt
      421 caaaatattt tcattcttca ttcttcattc ttatctgtta ttattttata aaatatcact
      481 taattagagg taattttagt attgttttta gtccctgact atcaatactc ataatatcta
      541 atttacctct atgtgcatta ataattccag ctactatcaa aatacctaga ccatgatttt
      601 gacctttggc atatgcctca cttagtccat tttggctctt taataaacta ttaattcttt
      661 cttgactcat accttttcca tcatcttcaa caattatata gcaaaaatct aaatcagact
      721 tagaggtaac ccttatatta catccttgtt ggttatgagt tatactattt tgtattaaat
      781 tactaatagc cctttttaat aattttttat ctgcatctat aataatatct tcatttagta
      841 tatcaagttc aacataaaag tcattatcca aattattatt tataaactca gaaataagct
      901 ctctcaaaaa cgcagaaact ctaatcttat cacaattcaa tgcttgcata ttatattcaa
      961 gtttagatac taaatttagg tcatttacta attctctgat tttaacccct tgattgcata
     1021 taatagacac ttgctcttgt tctctatttg aaaggctact gctttcttca agttctccag
     1081 catatccaat tatcattgaa agtggagttc ttatatcatg agatattcct gcaatccagt
     1141 tagacctagc ttcctctttc ttcctaagtt tttcatcttt catttgcaac tctttagata
     1201 cagtattaat agattttgaa atatcactta gtattccttt ttcttgtaaa agtactggtt
     1261 ctccttttga cataagcttt atgccagtaa taattggttt tatagatttc accattttta
     1321 aacctatgaa tagagctatt aaaaaagtaa ctattgcatt aaccagaaac atacctatta
     1381 ttaacatagg aaattgttct atttccttaa tcgtataagt aaggttaaat ttagtgtatt
     1441 tgtcctttgg ataacctgtt actaatattc catttggatg ttcccataca tacacaggat
     1501 agtcctttaa aaagaacctt gaaaactttg ctacatctgt caaagtataa tgacttggaa
     1561 tttcttttgg taaatcaaat ccccatattc tatctccatt gttatcaatt aacattgccc
     1621 aaactttatt tttatgtagt acttccttac tagaatcatc taaaataaac tttccatctt
     1681 ttaaaatcaa attatcttca atcataatca agtttgattt tatttctctg ctatcacttg
     1741 tatctaaatt atcagagtat ttagatacaa atagcatata tgaaaacaga agaaaacctg
     1801 ctatatttaa tacaataaaa agtaatgaaa tcaagatact tgaacctata aatcgcctca
     1861 atatgtactt tacactatcc aaaattaaat cactcctctt gatttctttt attctaatat
     1921 ctaaaattaa ttaatcatca acttatatcc aagacctctt actgtaataa gatgtttagg
     1981 tgtagatgga gttttttcta tcttctctct aacccttcta acatgaacca taagggtatt
     2041 ttcatatcca tacatatcat ctccccaaat ggcttgacat agtgaatcat tagtaacaat
     2101 tctcccttta ttttcataca actttaaaag caacgaatgc tctttagctg ttaaagaaat
     2161 ctcttgccca tctttctcaa cacatgcact atttaaattc acaacaacag aatctcccag
     2221 tttaaatact ggcaactcct cttcttctat aggcacatat acacgattta atatacccac
     2281 aagccttaaa gtaagttcct ttggtaaaaa tggctttact atataatcgt cagcccctaa
     2341 tcccaaacca atcaatctat cttcatcttc acctcttgca gagagaaaaa taactggtat
     2401 cttagatgtc tctctaattt ttctcataat agagaaacca tctccatctg gcaatacaac
     2461 atctaaaatt gcacaatctg gctttacatt tctaaatacc ctaacagctt cttcataact
     2521 tgatgctaaa aatactctac taaatccctc ttttcttagg atttcatcta tcatttttaa
     2581 taattccttg tggtcatcaa ccacaagtat ttttttatct tttattgact ccataatcat
     2641 caccctcctt tagtatacca tatacccatt ttcacaactt tgcctaaata gacttttaag
     2701 gttattgtaa ggtaaacttc agctaactga aatgtaacca agttattata ctgatataag
     2761 ttaaaagaat caataagcac agattaaaaa aggggatgaa acatatggaa atgataatta
     2821 gaacacaaag actttcaaag gaatacaata aaatatttag agtaaaggat atagacctaa
     2881 gagttccaca aggagctgta tatggttttt tgggtcctaa tggagctgga aaatctacca
     2941 ctcttaaaat gcttcttgga cttgctaaac ccacacaagg aaatattaat attttaggta
     3001 aagagttaaa tgaaaaaaat agaatatcaa ttttaaaaga tataggttct ttgatagaat
     3061 ctccatcata ctatggtcat cttacagggc ttgaaaacat gaccataatg caaagactct
     3121 tagatttacc taaaaaaaat gtaaatgaag cattaaaaat tgtaaggtta gaaaatcaaa
     3181 aaaataaaaa agttaatcaa tattctttag gtatgaagca acgtcttggc attgctatgg
     3241 caataatgaa gtttcccaaa ttattaatac ttgatgaacc tacaaatgga ctcgacccag
     3301 cagggattga ggaaattaga gaattaataa aatctctacc agaaaaatac ggcatgactg
     3361 ttctaatctc tagccatctt ttaagtgaaa ttgaccaaat tgccacatca ataggaataa
     3421 taaatcatgg cgaactggtt tttcaagata gcataaaaga acttcataat agaggtcaaa
     3481 gtcagattgc tatcaaagct aagaatgttg tatctgcaca aaagctatta aatactaaag
     3541 gatatatgtc acttatacaa aatgggtact tgactattga aaatttaaaa gactcagatg
     3601 tggctaaaat aaatggtatg cttgtccatt caaatataga tgtatttaga atagaagaac
     3661 ataaaaaaag cttggaagat atattcctag aacttactgg taaggcggtg actttataat
     3721 gaaatacctt ggtctagaat tttataaact taaaagaaag aagattattc tgatgacctt
     3781 tttatttgta gctgttgaaa taatgtggtg tatcgtaaat tcaaataggg ctattacacg
     3841 taatccagat atgttaggtg gatttgaata ttcctatatg cttatgagtt ttgcaagttt
     3901 aaatggtcta ttctttccta tcttaatatc aataattaca tcacgcataa gcgatataga
     3961 acataaagga gatacatgga agcttttaaa atcttctgtg acatctttaa atagtattta
     4021 tttatccaaa tttttatgtt ctgctatttt ggtatctata cctatattga tgcaagtgtt
     4081 atctattgtg ttgtttggac attttagagg ggttattgag tcaatttctg tatctttact
     4141 tttaaaatac acattaggaa caattcttgt aagtattgct atcattgcac tacacctatg
     4201 gattgcaact gtaattccaa atcaaatgat tgcaattaca tttggcatga ttggtagttt
     4261 tataggtttg acatcaggtt tgttttatca aggtattcgt agattgttta tatggtctta
     4321 ttacctagag ttatcacctt taagttatgc ttatgataat gttttaggtt catctgtcta
     4381 tagaattgat atgaattttt ctattacgat tttagttttc ttaattggaa tgctaattta
     4441 ttatattgga agaaatcatt tattaaaaaa agaagtatag gagggattta aatgttaagg
     4501 aagtcagttt tgggagaaat tctaaagtta aaaaattctt ttatatggta tgtattatta
     4561 gctttgccac ttataagtgt gttgattggt tctggtaatt tttatctaaa tcaaggcata
     4621 ctcaaaaaag aatggtacag cttatggact caagttagtc ttttttatgg ggaatttttc
     4681 tttcctatct taatcgcaat cttttgtgca tatatttgta ggcttgaaca tatgaatcat
     4741 aattggaata atgtaatgac actgccagtt aatttaaaaa atatcttttt atctaaactt
     4801 acagtagttt caatacttac agctattaca caagttttct ttgttatctt ttatatagtt
     4861 gcaggaaata tgtttggttt ttctggtagt gtacctaaag aattgactgg ttggattgct
     4921 aaaggttgga ttatttctat ttcaatagct tctatacaat tatacatgtc tataaagatt
     4981 agaagttttg caactcctat tggtattagt ctttgccttt ctctatttgg tatgggatta
     5041 atagtagcaa aacctgctgt tggtatattt ttcccttatt ctatgttagg tagtgctatg
     5101 ggggttataa agcaatctgc tttaaatgtt aatgacacta tagttacaat atgtgttgca
     5161 tttattacta ctatagtatt ttctgaaatt gccaatagaa attttagaaa gaatgatgtt
     5221 atttcttaat ggtaaatgct agatattttt tggtttgaaa tttatattct ttttggtttg
     5281 aaatttatat cccttttatt taaaaatata tatcttttat ttgaatttt
//
LOCUS       DAMJWP010000232         5326 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.239, whole genome shotgun sequence.
ACCESSION   DAMJWP010000232 DAMJWP010000000
VERSION     DAMJWP010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5326
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.239"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            9..911
                     /locus_tag="PC700_003202"
     CDS             9..911
                     /locus_tag="PC700_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HDF2582472.1"
                     /translation="MLPQDYYGLIVILGVILGVMGAFYNWFTLYVQSLYKKIPKIATF
                     GKLLIPFIMAGILGIVMPDVLGSGHNLVEELTSHEYIFGMVVLIFVIRFIFSAVSFGS
                     GAPGGIFFPLLVLGAFIGGIFGMVGVKVFGMNPDYVNNFVLLAMAGYFTAIVRAPLTG
                     IILIFEMTGSVSQMLSLSVISIVAYIVATLMKSKPIYESLLERLLEKNGEPVHKERGE
                     KILDQFSVMFGSPVANKAIQDVQWPENCLLVAIQRGGEEIIPRGKTILRPSDVIVTMT
                     DERDSGAVYDKMENLCKEHTSHIF"
     gene            1520..2533
                     /locus_tag="PC700_003203"
     CDS             1520..2533
                     /locus_tag="PC700_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HDF2582473.1"
                     /translation="MVSSFLNDIFRTILVSCIFIVILLVFRITLFKTFSKKFNYYIWL
                     IVIIKLSLPFMNYTFIFNESKYNGNINKISLGNFNSISIVYSTVLVSVWIAITIFYLV
                     YTLLKYIKLKNMINDLSYDVEDDKIKNLYENLLKELKINKKIRLKYSYEVESPVFFNS
                     CVILPPCDYTLKELNWIFRHELMHFKSKDLYIKYLVIFLKCVYWFNPFVYIMEKFIDL
                     DCEFYCDERVLKNCTIEEKQDYALTIISAMRKGSNSSNKFVAGLHKESDIKKRVFSMF
                     NEKYRNGILIAVILCLLSSITYFEIDLITTKSIFYPLSKQTPIENRTNSKPTYQINVY
                     IDN"
     gene            2889..3458
                     /locus_tag="PC700_003204"
     CDS             2889..3458
                     /locus_tag="PC700_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HDF2582474.1"
                     /translation="MKKKLEDMTLEELWNLFPIFLTTHKECWKNWFKEEQYRLTGILP
                     MQKIVRISHIGSTSVSTIYAKSIVDILIEIIPDYNMKDIKDLIESNGYICMSESSSRL
                     SLNKGYTVDGFEERVFHLHIRYVGDNDELFFRDYLIDHPDVAKEYEKLKLDLWKKYEH
                     NRDAYTDSKLDFVREFTEKAKVLYSNRYN"
     gene            complement(3795..5132)
                     /locus_tag="PC700_003205"
     CDS             complement(3795..5132)
                     /locus_tag="PC700_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2582475.1"
                     /translation="MDTYSLISTSINFILILINIFIAFFLYKLTSYFIDFRNHWIFKI
                     ILILGYSIISQMIIWLSDPVNILFTLFGYFILLIICSKSKLIPIISVVMILYPIIIGL
                     NFLFINNPIYEELIHSKEYILILSIVQIISALIKLLFWFLIHIFLKNRLVNIKQYLSN
                     KIWIFIDMVCISSFLSILIAIILPPTNLIPMGSNKNIILGNLPYGTYLIILSAILANI
                     GVILLLQPLIENVKMKIEKQANHLKEKYYNLLENQQIQIRKMRHDMNNHFQMIESYLE
                     IEDIAGAKKYFNQLKIGIGTLSGKQFCKNQALNSILNLRHTSLEENGVDVHFNIDIDN
                     HLGIEPIDLCTIFSNSLDNAIEASLKIPDLSNRKVVLKARCEKEYFSYLLTNNKVNPI
                     NRKQELFISDKKDSNSHGYGIENIREIVNKYNGKLDISYTENEFSLFLYIRLF"
BASE COUNT         1971 a          525 c          866 g         1964 t
ORIGIN      
        1 ttggaaatgt gttgccacaa gattattatg gattaatagt gattttaggt gttattcttg
       61 gagttatggg ggcattttac aactggttta ctctatatgt gcagtcttta tacaagaaga
      121 ttcctaaaat tgctaccttt ggaaaactgt tgattccatt tataatggct ggtattcttg
      181 ggattgttat gcctgatgtt ctggggagtg gacacaattt agttgaagaa cttacaagtc
      241 atgaatatat ttttggaatg gttgttttga tttttgttat acgttttata ttttcggctg
      301 ttagttttgg gtcaggtgca ccagggggaa tattttttcc attgcttgtt ttaggagcat
      361 ttataggtgg aatatttggt atggtaggtg ttaaagtttt tggaatgaat cccgattatg
      421 taaataactt tgtgcttttg gctatggcag gatattttac agctattgtg cgtgcccctt
      481 tgactggtat catattaatt tttgaaatga caggttctgt tagtcaaatg ctttcactat
      541 cagtaatttc aattgtggca tatattgtgg ctacacttat gaagtctaaa cctatttatg
      601 aaagtcttct tgagaggtta cttgaaaaaa atggagaacc tgtacataaa gagaggggcg
      661 aaaagatact agaccagttc tctgttatgt ttggctctcc tgttgcgaac aaggcaattc
      721 aggatgtaca gtggcctgaa aattgtcttt tggtagcaat tcaaagaggt ggagaagaaa
      781 ttattcctag aggtaagaca attcttagac caagtgatgt aattgtgact atgacagatg
      841 agagagatag tggtgctgtg tatgataaga tggaaaattt gtgtaaagaa catacgtcac
      901 atatttttta gggataatga tgaatgttta ataatgatag aatatttaat gaagatttga
      961 ttaaaaaatt ggtaatttga aagagagtta aggaattaat agaaaatgaa aatgtgatat
     1021 cttagatgta aaagagtttg aagaaaataa ttgtatatat ttgttaatga gattatatgg
     1081 actaatggta tatttatact gtgctaaaag ttgcactatt gtatgaggaa tgggaataaa
     1141 gggtttgaaa aaataggtag aagatttttc agatattctt attgggatat tttaattaat
     1201 atattttaat tgcgatattt tgggaagaat agaatttatt tataaatgaa aataattaaa
     1261 aggggttaag gttgtattag ccccttttgc ttatagagtt tggaaatggt atcttgatag
     1321 cagttgtttt atgcttatta tattttatta catattttaa aattaacctt atcactccta
     1381 aatcattatt ctatcaactt ttaaaacact ttcaagacaa tcacctattg aaaacaaagt
     1441 taattaaaaa cctacatatc agtttagtat tcgtgtagat gattatataa atataaaagt
     1501 ataagaaagg tgagtatctg tggttagttc atttttaaat gatatcttta gaacaatttt
     1561 agtaagttgt atttttatag ttattttatt agtctttaga ataactttat ttaagacttt
     1621 cagtaaaaaa tttaattact atatatggct tattgtgatt attaagctat cattaccatt
     1681 tatgaattat acttttatat ttaatgagtc aaaatataat ggtaatatta ataagataag
     1741 tttaggcaat tttaatagta tttctattgt atatagtact gttttagtct ctgtttggat
     1801 tgctattacg atattttatt tagtttatac tttattgaaa tatattaagc ttaaaaatat
     1861 gataaatgat ttgtcttatg atgttgagga tgataagata aaaaatcttt atgaaaattt
     1921 attaaaagag cttaagataa ataaaaaaat acggcttaaa tattcatatg aagttgaaag
     1981 tccagtattt tttaattcgt gtgtcatttt accaccatgt gattatacat taaaagagtt
     2041 aaactggata tttaggcatg agttgatgca ttttaagagt aaagaccttt acattaagta
     2101 tttggtgata tttttaaaat gtgtttattg gtttaatcct tttgtgtata taatggagaa
     2161 gtttatagat ttggattgtg aattttattg tgatgaaaga gttttgaaga attgtacgat
     2221 agaagagaag caggattatg cattgacaat aattagtgca atgagaaaag gttcaaatag
     2281 ttcaaataaa tttgtggctg gattacataa ggagtcagat attaaaaaaa gagtgtttag
     2341 tatgtttaat gaaaagtaca gaaatggtat tttgatagca gttattttat gtttattatc
     2401 ttctattaca tattttgaaa ttgatttaat tactactaaa tcaatatttt atccactttc
     2461 aaaacaaaca cctattgaaa atagaactaa ttcaaaacct acatatcaga ttaatgttta
     2521 tatagataat taaaaaatac aaagtttaac aagagattaa tataaataat tttaataagt
     2581 atctttcaca tatgttaaat ttactttaat tatattaaag ccaatgaatt attctttaga
     2641 agctccaatg ccagtaaata caggtaaaga aaaatctttt actacaattg taaattaaaa
     2701 aatattctaa tatttaaaga aatgcaaaag tgtaagaagg gtaaaaatct gttataatac
     2761 attaaaaact aaatttagaa aaatttacat aattagaaaa atgatttgat aaaaaataaa
     2821 gttaaatatt aactaagtaa atttgaaaaa gaaaaaatat aaagtacaaa tttaaaagga
     2881 ggtaactaat gaaaaagaag ttggaagata tgactttaga ggagttatgg aacttgtttc
     2941 ctatattttt gactactcac aaagaatgtt ggaaaaattg gtttaaagaa gaacaatata
     3001 gactaactgg aatattgcca atgcaaaaaa ttgttagaat aagtcatatt ggaagtactt
     3061 ctgtgagtac gatttatgcc aagtccattg ttgacatact gattgagata attcctgatt
     3121 ataatatgaa agatattaaa gatttgattg agtcgaatgg atatatttgt atgtctgaga
     3181 gcagtagtag gctttcactt aataaagggt atacagtaga tgggtttgaa gaacgcgttt
     3241 ttcatttgca tataaggtat gtaggtgata atgatgagtt gttttttaga gattacttaa
     3301 ttgaccaccc agatgttgca aaggagtatg aaaaacttaa gttggacctt tggaagaagt
     3361 atgagcataa tcgtgatgct tatacagata gtaaactaga ttttgttagg gagtttactg
     3421 aaaaggcaaa ggtgttgtat tctaatagat ataattagaa ataggtacaa ttagatattt
     3481 atcttatata tcgtattata ttttttaaga aagtaattaa tagaaaaagg ggttgtttca
     3541 aaatggagag ataaatccct ttttcattgt tgaaatgata aaaatatatt tgatttcatg
     3601 taaaaaagag tgcctcatca actaaaaagt ttattttgag acacacacat ttttattaag
     3661 gtaaataaaa atatcacatt tgattaaatt ttaagttaat gaatatatgt gtaaatattt
     3721 aattataagt tattataatt ttacatagta taaaacaatc taatttgtat tatataagat
     3781 aatctaattt tagactaaaa taatctaata taaagaaata gagaaaattc attctcagta
     3841 taagagatat ccaattttcc attgtactta ttaacaattt ctctaatatt ctcaatacca
     3901 tatccgtgag aattactatc ttttttatct gaaataaata gttcttgttt tctattgata
     3961 ggatttacct tgttgtttgt caataaatag ctaaaatact ccttctcaca acgtgccttt
     4021 aataccactt ttctatttga caaatcagga attttcaaag atgcttctat agcgttatct
     4081 aaagaatttg aaaaaatagt acataaatct ataggttcta tacccaagtg attgtctata
     4141 tctatattaa agtgcacatc aacaccattt tcttccaaac ttgtatgacg aagatttaaa
     4201 attgagttaa gagcttggtt tttgcaaaat tgtttgccac taagagtacc tatacctatt
     4261 tttaactgat taaaatactt tttagctcca gcaatatctt caatttctaa atacgattca
     4321 atcatttgaa agtgattatt catatcatgt cgcattttgc gtatttgaat ttgttgattt
     4381 tctagtaagt tatagtattt ttcttttagg tgatttgctt gtttttctat cttcatcttt
     4441 acattttcaa tcaatggctg taaaagaagt atcacaccga tatttgccag aattgctgac
     4501 aagatgatta gataagtgcc ataaggtaag ttgcctaaga taatattctt attactaccc
     4561 atagggatta aattagtagg tggcagaata atagcaatta atattgataa aaatgaagat
     4621 atacatacca tatcaataaa aatccatatt ttatttgata agtattgttt tatattgacc
     4681 aatctattct ttaggaaaat atgtattaaa aaccaaaata ataatttaat tagagcagaa
     4741 attatctgga ctattgaaag tattaaaata tattcttttg agtgtataag ttcctcatat
     4801 atagggttgt ttataaaaag aaaattaagc ccaattataa taggataaag tatcatgaca
     4861 actgatatta taggaattaa tttgcttttg ctacatatta taagtagtat aaaatatccg
     4921 aatagtgtaa acaaaatatt tacggggtcg gaaagccata ttatcatttg actaataata
     4981 gagtagccca aaatcaatat gattttgaaa atccaatgat tcctaaagtc tataaaatag
     5041 gaagtcaact tataaagaaa aaaggctatg aaaatattga ttaatattag aataaaattt
     5101 atgcttgtag agattaaaga ataagtgtcc atttaatata cctttctatt aattaagatt
     5161 ctggaaacta agttttaaaa aattaaatct taaaaattaa aattctggaa attagatttc
     5221 aaagaattga ttttgaaaaa taaaattcta gaaattaagc ttcaaaaaat tgagttttga
     5281 aagattgaaa ttttaaaaat taagtttcaa aaaattaaat ttgaaa
//
LOCUS       DAMJWP010000233         5308 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.240, whole genome shotgun sequence.
ACCESSION   DAMJWP010000233 DAMJWP010000000
VERSION     DAMJWP010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5308
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.240"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            102..938
                     /locus_tag="PC700_003206"
     CDS             102..938
                     /locus_tag="PC700_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2582476.1"
                     /translation="MNIKKIFNNNVVVSSLEDGTEIIVTGAGVGFKKKVGDLIDENLI
                     SKKYFVQDDQRDKYNQILNKTSIEYFKISEEIIEKANEVLNTQVNDSIILALTSHIEF
                     AVQREKQGIKLPNLILNETKQLYREEFKFGLWAIDEIEKRIGIKLPEDEAGYIAIHII
                     NGLENSQKDEGINILEFSKQVIQIIEEVHGFKLDVNSLNYTRLITHLKFFVQRILRKE
                     TYNDSDIEDMYKLVNKNYNKPKVCTEEIAALVLDRFEYKISKEEELYLMIHINKITNS
                     GC"
     gene            1291..2868
                     /locus_tag="PC700_003207"
     CDS             1291..2868
                     /locus_tag="PC700_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2582477.1"
                     /translation="MKKNKLGLWNFFQMLGKTFMFPIALLSVSGMMLGLGAGFTDPKM
                     IEMVPFLGNEYVNLVLNFMLTIGLFAFNNLGALFAMAIPLGLLKKEKEFGAFSGLVGF
                     IAMHIGTNFYLTRADLLVPAEQMSTNGQAMIMGIQTYNTSVLGGIVAGLIVYAIYDKV
                     SNLKIPESLGFYSGPRLVPIVSLIVMSIVGLLIPIVWPPFFNAFQSLGRWISSAGPLG
                     YFSYAVAERVTIPFGLNHLVTSVFRFTPIGGTAVIDGETYFGTLNMFMAYVENNQIIP
                     LDLAGKMEQGKLMIQYGLAGAALAIYRTAKPENRKALKGLLISGVLTVIIGGISEPIE
                     FLFLFISPALFAFHTFMNGLANMVLPYLGVLMGFTGDLIAFISFGVLRGTATGWPIAV
                     LVAVLYFAIYYFVFKWAIIKFNIKTPGREDKEIKVSEGNGSDFKNLSTYKGQQMISAL
                     GGQSNILSLDNCVTRLRLKLQDVSLINEDAIKEAGGIAVVKLDEHTIQVIIGTQVYSL
                     RKQMDKAMEGHLEACND"
     gene            2861..4045
                     /locus_tag="PC700_003208"
     CDS             2861..4045
                     /locus_tag="PC700_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2582478.1"
                     /translation="MINFDKKVDRLGTYCTQWDYVEDRFGKKDLLPFTISDMDLEFPF
                     EITEVLQERLNHRVLGYSRWKHDDFRNSVTNWYIKRFNCSVDSNWIYYSPSVMYSISK
                     LIEMLSNEGDGILINTPAYNAFYDVITNNNRNIIKSHLVNRNGFYSIDFNDFEKKCKK
                     SKIFILCNPHNPTGRVWSELELVKMIGICKNNDVKIISDDIHMDIVYDNRMTPILNVA
                     KDYLDSVFICTSASKSFNIPALTGSYVIIPNEGIRNKFENITRYRDFVNSPAILAILA
                     TMVCYDDCEYWLDELLKYLKENLKYTIDYIKDNLEPLKLVMPEGCYFAWIDFSELKIS
                     GEKFQKLLMDIGEVAIMSGSVYGEEGEFYLRLNVACSREKLEDGLNRIKKTMDYIAKR
                     TL"
     gene            4063..4662
                     /locus_tag="PC700_003209"
     CDS             4063..4662
                     /locus_tag="PC700_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF2582479.1"
                     /translation="MGYLKTMVDVMTTEMNAIRCLIDEAGIEYESIVSEIANCEGKVI
                     FMGVGKSAHIGKKLAATFASTGTPSFFVHATEAVHGDLGMIESKDITILISNSGNSME
                     VVNCIKYIKAIGSKTIAFTSNRNSVLAKECDYALIYPAKDEADHLNLAPTTSSTITLV
                     LGDSIACALSKSSNFGSSDFYKYHPGGSLGEKLKTANNG"
     gene            4686..5156
                     /locus_tag="PC700_003210"
     CDS             4686..5156
                     /locus_tag="PC700_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2582480.1"
                     /translation="MFKMFKKKSIKSPISGKVIELSKIQDGVFSQKIMGEGVAIDSTG
                     DIVYAPINGKIVVVAETKHAFVIELENGMELLIHVGLDTVNLKGEGFEALVSMGEQVK
                     EGTPMLKINRPLIELKGISLITPVTITNHSEYNMNTCNVGNSVEGGKDTVIEFK"
BASE COUNT         1982 a          562 c         1021 g         1743 t
ORIGIN      
        1 tgcaaaatat atacgaaagt aaaattttaa atattgacat taaattaaat atactatact
       61 atgtaagtgt gatgagagaa tttttttaga ggggatttat agtgaatata aaaaagatat
      121 ttaataacaa tgtagtagta agctccctag aagatggcac agaaataatt gtcactggtg
      181 ctggagtagg atttaagaaa aaagtaggcg atttgataga tgaaaatttg atttctaaga
      241 aatattttgt tcaagacgat caaagggaca aatacaatca aatacttaat aagacctcta
      301 ttgaatactt taaaatttca gaagaaatta tagaaaaggc taatgaagtt ttaaatactc
      361 aagtgaatga ttcaataata ttagccttaa ctagtcacat agaatttgct gtccaaagag
      421 aaaaacaagg tataaaatta ccaaacttaa tattgaatga gacaaaacaa ctttataggg
      481 aagaatttaa atttggatta tgggcaattg atgaaattga aaaaaggata ggaataaaat
      541 tgcctgaaga tgaagcagga tatattgcta ttcacataat caatggtttg gaaaattctc
      601 aaaaagatga aggtattaat atactagagt tttcaaaaca agttattcag ataattgaag
      661 aagtacatgg atttaaattg gatgtaaatt cattaaatta tactagactt ataactcact
      721 taaagttttt tgttcaaagg attctccgaa aagaaactta caatgatagt gatattgaag
      781 atatgtataa gttagtgaat aaaaattaca ataaacctaa agtgtgtact gaagaaattg
      841 cagcattggt attagacaga tttgaatata agattagtaa agaagaagaa ttatatttaa
      901 tgattcacat aaataaaata acaaacagtg gttgttagga ttgtaactga taaagcaggc
      961 aaaacctaag tcaatgagct taactattag attaagtttt ttgatttagg tttttttatt
     1021 agatacaatc aaaatacaga cagtgacttt taggattgta actggttaag caggcaaaac
     1081 ctaaatcatt gaatttaact attaggttaa gttttttgat ttaggttttt ttattagata
     1141 caatcaaata cagacagtga cttttaggat tgtaactggt tgagcaggca aaacctaaat
     1201 cattaaactt aattattaaa ttaagttttt tgatttaggt ttttctataa atttgattat
     1261 gtttttagat aaaaatagga ggggaaaatt atgaaaaaaa ataagttagg tttatggaac
     1321 ttcttccaaa tgttagggaa aacgtttatg ttcccaatag cactacttag tgtttctggg
     1381 atgatgctag gacttggggc aggttttaca gaccctaaga tgattgaaat ggttccattt
     1441 ctagggaatg aatatgttaa cttagtttta aacttcatgt taacaatagg attatttgca
     1501 tttaacaact tgggagcatt atttgcaatg gcaattccac taggattatt aaaaaaagaa
     1561 aaagagtttg gtgctttttc aggattagta ggatttatag caatgcatat aggtacaaat
     1621 ttttatttaa caagagctga tttgttagta ccagcagaac agatgtcaac taatggtcaa
     1681 gctatgatta tgggtataca aacatataat acaagtgttt taggtggtat tgtagcagga
     1741 ttaattgttt atgcaatata tgataaagtt agtaatttaa agataccaga atcattagga
     1801 ttctacagtg gaccaagatt agttccaatt gttagtttaa tagttatgag tatagttgga
     1861 ttattaatac ctattgtttg gccaccattt ttcaatgcat tccaaagttt aggaagatgg
     1921 atttcttcag caggaccatt aggatatttt tcatatgcag tggcagaaag agttacaatt
     1981 ccatttggac taaatcatct tgtaacatct gtattccgtt ttacaccaat aggtggaact
     2041 gctgttattg atggagaaac gtactttggt acattgaaca tgtttatggc atatgtagaa
     2101 aataatcaga ttatacctct tgatttagct ggtaaaatgg aacaaggtaa attgatgatt
     2161 caatatggat tagctggtgc tgcattggct atctaccgta cagcaaaacc agaaaataga
     2221 aaggcactta aaggactttt aatatcagga gtgttgacag ttattatagg tggtataagt
     2281 gaaccaatag agtttctatt cttattcata tcaccagcat tatttgcttt ccatacattt
     2341 atgaatggtc tagcaaacat ggtacttcca tacttaggcg tgttgatggg attcacagga
     2401 gatttaattg catttataag ttttggtgtg ttaagaggaa cagctacagg atggccaata
     2461 gcagtattag tagcagtttt atattttgca atatactatt ttgtatttaa gtgggcaatt
     2521 attaagttta atataaagac cccaggacgt gaggataaag aaataaaagt atctgaagga
     2581 aatggttcag atttcaaaaa tttgtctaca tataaaggac aacaaatgat atctgcttta
     2641 ggtggtcaat ctaatatatt atcactagat aattgtgtaa ctagattgcg tttaaaacta
     2701 caagatgtat cattaataaa tgaggatgca attaaagagg ctggtggtat agcagttgtt
     2761 aaattggatg agcatacaat acaagtaatt ataggaactc aagtgtattc tttaagaaag
     2821 cagatggata aagctatgga aggtcatttg gaggcatgta atgattaatt ttgataaaaa
     2881 agtagaccga ttgggtacat attgtaccca gtgggactat gtagaagata gatttggtaa
     2941 gaaggatttg cttcctttta caatttcaga tatggattta gagtttccat ttgaaataac
     3001 agaggtgttg caggaaaggt taaatcatag agttttggga tacagtagat ggaaacatga
     3061 tgattttaga aattcagtta cgaattggta tattaaaaga tttaattgta gcgttgatag
     3121 taactggata tattatagtc caagtgtaat gtattcaatt agcaaactta ttgagatgtt
     3181 aagtaatgaa ggagatggaa tccttataaa tactcctgca tacaatgctt tttatgatgt
     3241 gataacaaat aacaatcgta atattataaa gtcacatctt gtaaatagga atggattcta
     3301 ttcaattgat tttaatgatt ttgagaaaaa gtgtaaaaaa tctaagatat ttattttatg
     3361 taatcctcac aatccaactg gtagagtatg gagtgagtta gaacttgtta agatgatagg
     3421 aatttgtaag aataatgatg tcaagataat atcagatgat atacacatgg atattgtata
     3481 tgacaataga atgacaccta tcttaaatgt tgcaaaggac tatttagatt ctgtattcat
     3541 ttgtacatca gcgtctaaat ctttcaatat accagcattg actgggtcat atgtgattat
     3601 accaaatgaa ggtattagaa ataaatttga aaatatcact agataccgtg attttgtaaa
     3661 ttcgccagca attctagcaa tattagctac aatggtctgt tatgatgact gcgaatattg
     3721 gttagatgaa ctattaaaat accttaaaga gaatcttaaa tatacgattg attatataaa
     3781 agataattta gaaccattga aattggtcat gccagaaggc tgttactttg cttggataga
     3841 tttttccgaa cttaaaataa gtggtgagaa attccaaaaa ttattgatgg atattggaga
     3901 agttgctatt atgtcggggt ctgtatatgg tgaagaaggt gagttttatt taaggttaaa
     3961 tgtggcatgt tcaagagaaa aacttgaaga tggtttaaat aggattaaaa aaactatgga
     4021 ttatattgca aaaagaactt tatagtagga agggagatag ttatgggata tttaaaaaca
     4081 atggttgatg ttatgactac tgaaatgaat gccataagat gtttaataga tgaagcagga
     4141 atagaatatg aaagtatagt tagtgaaatt gcaaattgtg aaggcaaagt aatttttatg
     4201 ggtgtaggaa aatctgcaca tataggtaag aaattggcag ctacatttgc tagtactggt
     4261 acacctagct tttttgtaca tgcaacagag gcagtgcatg gagatttagg aatgattgaa
     4321 agtaaggata ttacaatttt aatatcaaat agtggaaatt ctatggaagt ggtaaactgt
     4381 ataaaatata ttaaagcaat aggttctaag acaattgcat ttacttcaaa cagaaattca
     4441 gtactggcaa aagagtgtga ttatgcttta atatatcctg caaaggatga agcagaccat
     4501 ttaaatctag caccaacaac atcatctaca attactttgg ttttaggaga ttctattgca
     4561 tgtgcattat ctaagagtag taattttggt tcttcagatt tttataagta ccatccagga
     4621 ggaagtttag gtgagaagtt aaaaacagct aacaatggat aaaagcaaaa aggagagtag
     4681 tttttatgtt caaaatgttt aaaaaaaaat caataaaatc acccatatca ggaaaagtaa
     4741 tagagttatc taaaatacaa gatggtgtat tttctcaaaa aattatggga gagggtgtag
     4801 ctatagattc tacaggagac attgtatatg ctccaatcaa tggaaagata gtagtagtag
     4861 cagagactaa acatgcattt gtaatagaat tagaaaatgg gatggaactt cttattcatg
     4921 ttggtttaga tactgtaaat ttaaaaggcg aaggttttga ggcattggtg agtatgggag
     4981 aacaagtgaa ggaaggaaca ccaatgttga aaattaaccg tcctttgatt gaattaaagg
     5041 gtatatcact tattactcca gttacaataa ctaatcattc agagtacaat atgaatacat
     5101 gtaatgtagg caatagcgtt gaaggtggga aagatactgt aattgagttt aaataaagaa
     5161 ttaaaataca aggttaatat ttataaatac ctataaactt atgttttata agattgattg
     5221 tatggattta ttgtataaaa aagaactctt taattataaa ttaaggagtt tttttatttt
     5281 gtaaatattg tttagattta tatagaaa
//
LOCUS       DAMJWP010000234         5244 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.241, whole genome shotgun sequence.
ACCESSION   DAMJWP010000234 DAMJWP010000000
VERSION     DAMJWP010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5244
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.241"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            251..2263
                     /locus_tag="PC700_003211"
     CDS             251..2263
                     /locus_tag="PC700_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2582481.1"
                     /translation="MKKSVALVNDSRKDLIDFLENNLKLVFGDSININRYFINEINDN
                     DIINDDVILVMSVERLDKIINNILDKKKVIVVRRTFREDKIYNLLSLPQGTNALIVND
                     SDETTLETISLFYKIGVTNIRPIPYMNDNNYKNIKIAITPGVPEKVPSFISDIFDLGH
                     RYIDISTFIEIINLLQIDSKEIQSNLVKYSEEIISLDTGIKDKYKELFLKIEELDTIL
                     NLSKDGILFTSKDGEINTYNSKVKDILDINEDIYGKYIDDIFVDSLKVLLSEKEILDK
                     VVVFNKKYINVNKKNIYNRDEKMGTYYSLQEITYIKKLEQNLTKKLREKGQIAKYTFK
                     DIKTNSPKMFECIDLAKKVSKSDLSILIRGESGTGKELIAQSIHNNSNRKNQPFIAVN
                     CAAVPENLLESQLFGYDKGTFTGGLKDGKQGLFELANNGTIFLDEIGDMPLELQTKLL
                     RVLQEKQIMPVGSHNVINIDVRIISATNKNLEQMIDNSQFREDLYYRLNTIPINIPPL
                     RERKEDILIIMEDLINKKLVITPEAKKLIQNYMWKGNIRELQNVTSYLNIMCEDIVLE
                     KDLPPNLRSSDNKNTSLKLKYSKNDILNILEILILNKESDVGIGRGLILKALLDKNLQ
                     ITEGKIKKIFEYLKKEELIICSSGRYGSKITQKGEDFYNKLKYKGL"
     gene            2632..3852
                     /locus_tag="PC700_003212"
     CDS             2632..3852
                     /locus_tag="PC700_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2582482.1"
                     /translation="MQQALKLKYQTKELGKDFVIESCNSIKPWLINIRRELHKIPELA
                     LEENLTKQKVISYLKEIGIDYMEFTKHNGIMAYILKESADKTICIRADMDALPIEEEN
                     NIPYKSIHSGKMHACGHDAHTTMLLGACKVLHSIKDKLNVNVKFLFQPAEEGFGGAKF
                     LVEDGCLENPKADYIFGLHVMPHIETGLIETKYDTLNASVDTIKISIKGKRAHGAYPE
                     NGIDAIVTASQIVTSLQTIISRNLEPNNAAVLTIGKIYGGDAHNVICEDVKLEGTLRT
                     LNSKTRNFMIDKIAKIVGDTASAFGCVGTLHVSDENYPAVINEKELVDTVISSTKELL
                     GEEKFILRPNPSLGGEDFSFYTEHCKGAFFHLGCKNEEKGLISPLHTSSFNIDEDCLP
                     IGVMMHVMNTLYFN"
     gene            3889..5238
                     /locus_tag="PC700_003213"
     CDS             3889..5238
                     /locus_tag="PC700_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2582483.1"
                     /translation="MEKAKKLGLFSMILLGINSIIGSGIFLLPGKVYSLAGQNSMFIY
                     IFATLLVLSILLCFAEVGSMFDKNGGAYLYSKKAFGDFIGFEVGTMSWFIRIISWSTL
                     AVGFATALGSFWPESATEYKGCIAAILVTLLSINSLFGIKSTKIMNNVITIAKLVPLI
                     VFVIVGIFFIKFVNIVPSGNVVNSSMGPAIILVFYAFTGFESFIVASGEMENPKKNLP
                     IALITTIFICAIIYILIQIVCMGILGDRLFENSIPIADASSVFLGNYGKVFISVATLI
                     SIFGINIGSSIVTPKCGSSLAEEGSLPAFIGKTNKYGAPYVAIIISLICCIPLVLTGS
                     FEQLAVMSVIARFAQYIPTCLSVIVLRKRTDVKASFKIPFGPVIPMVAILGSLWLLQQ
                     AWAEDISKPITQNRVLIGLGAMLLIAPLYIFMKKNKEIEHKNKSEDSKLYISREKIQ"
BASE COUNT         2136 a          586 c          813 g         1709 t
ORIGIN      
        1 atttttttgg gtgtatatta taatttaatc gaatagaaat gaaaaatata catacagttt
       61 aagttttaag caacaattag ggggatttgg tgacaagatt tttaattata agaaagaaag
      121 tgtcttaatg caacttttat aaagtcatta tagacacttt cttttttatt tgataaattt
      181 aaattatcaa aattacattg ctttatacaa ctattttgtt ggaaactatt tattatataa
      241 ggagaaataa atgaaaaaat cagtagctct ggtaaatgat agtagaaaag atttaataga
      301 ctttttagaa aataatttga aattagtttt tggagattct ataaatatta atagatactt
      361 cataaatgaa ataaatgata atgatattat aaatgatgat gtgatattag taatgtcagt
      421 agaaagatta gacaaaatta taaataatat actagataag aaaaaagtga ttgtggtaag
      481 aagaactttt agggaagata aaatttataa tctactatct ttaccacagg gtacaaatgc
      541 tctaatcgta aatgattctg atgaaactac tctagaaact ataagtttat tttataaaat
      601 tggagtgact aatattagac caattccata tatgaatgat aataactata aaaatataaa
      661 aatagctata actccaggag ttccagaaaa agtacctagc tttatctcag atatatttga
      721 tttaggtcac agatatatag atatttctac atttatagaa ataataaatt tattacaaat
      781 tgattcaaag gaaatacaat ctaatctagt taaatactca gaagaaataa taagtttaga
      841 tactggtata aaagataaat ataaggaatt atttttaaaa atagaagaat tagatacaat
      901 attgaattta tcaaaagatg gtatattatt tacttcaaaa gatggtgaaa taaatacata
      961 taatagtaaa gtaaaagata ttttagacat aaatgaggat atatatggaa aatacattga
     1021 tgatatattt gtagatagtt taaaagtttt attgagtgag aaagagatac tagataaagt
     1081 tgtcgtattc aataagaaat atataaatgt aaataaaaaa aacatataca atagggatga
     1141 aaaaatgggc acatattata gtttacaaga aataacatac ataaaaaaat tagaacaaaa
     1201 tctcactaaa aaattaagag aaaaaggaca aatagcaaaa tatacattta aggatataaa
     1261 aacaaatagt cctaaaatgt ttgaatgtat agatttagct aagaaggttt ctaaatctga
     1321 cttaagcata cttatcagag gtgaaagtgg aactggtaaa gaactcatag ctcagtctat
     1381 acataacaac tcaaatagaa aaaatcaacc ttttatagca gtaaattgtg cagccgttcc
     1441 tgaaaattta ttagaaagcc agctattcgg atatgacaag ggaactttta ctggaggtct
     1501 aaaagatgga aaacaaggat tatttgaact tgctaataat ggaacaatat tcttagatga
     1561 aataggcgat atgcctttag aattacaaac aaaactactt agagttcttc aagagaagca
     1621 gataatgcct gttgggtctc acaatgtaat taatatagat gttagaatca tatctgctac
     1681 aaataagaat ttagaacaaa tgattgataa tagccaattt agagaggatt tatattatcg
     1741 attaaataca attccaataa atatacctcc tctaagagaa agaaaagaag acatattaat
     1801 tattatggaa gatttaatta ataagaaatt agtaatcaca ccagaagcaa aaaaacttat
     1861 acaaaactat atgtggaaag gtaatataag agaactccaa aatgttacat cctatttaaa
     1921 cattatgtgt gaagatattg tattagagaa agatttacct ccaaacttaa ggtcttcaga
     1981 taacaaaaat acatcattaa aactaaaata tagtaagaat gatatattga atatattaga
     2041 gatactaatt ttaaataagg aatcagatgt tggtattggt aggggattaa ttttaaaagc
     2101 actactcgat aagaaccttc aaattacaga aggaaaaatt aaaaaaatat ttgaatatct
     2161 aaaaaaagaa gaacttataa tttgtagttc aggaagatat ggaagtaaaa taactcaaaa
     2221 aggagaagat ttttacaata aattaaaata taaaggtcta taaaattata atttatcaaa
     2281 caaagaaatc tattcaggat taaagtactc cttcaatagg tttttttatt tttcaagtac
     2341 cctaaaatta taatttattt tgtacataaa taataggaat taataggaat aggataattt
     2401 ttttatttcc tatttttttt atataaacta gcatataaaa tatattttct tcaaaataat
     2461 attttttact gaaaattgaa actatgcaat cattttttat actatacata tagataaatg
     2521 attatattta ttcaatattc ttataaaaaa caaaattctc catcgaaaat tatggcataa
     2581 taattgctta ttatattaat aaaacaataa aaagcaattg gaggaataaa aatgcaacag
     2641 gctttgaaat taaaatatca aaccaaagaa ttaggaaaag attttgtaat agaaagttgt
     2701 aattctataa aaccatggct tataaacata agaagagaat tacataaaat tccagaatta
     2761 gctcttgaag aaaatcttac taaacagaag gtaatttctt atttaaaaga aatcggaatt
     2821 gattatatgg aatttacaaa acacaatgga attatggcat atattttaaa agaaagtgca
     2881 gataaaacga tttgtataag agcagatatg gatgctcttc ctattgaaga agaaaacaac
     2941 ataccttata aatcaataca ttctggaaaa atgcatgctt gtggtcacga tgcacataca
     3001 accatgctac ttggagcatg taaagtttta cattcaataa aagataaact taatgtaaat
     3061 gtaaaattct tatttcaacc agcagaagaa ggttttggag gagcaaaatt tttagttgag
     3121 gatgggtgtt tagaaaaccc caaggccgat tatatattcg gacttcatgt tatgccacat
     3181 atagaaacag gccttatcga aacaaaatat gatacattaa atgctagtgt agacactata
     3241 aaaataagta tcaaaggtaa aagagctcat ggtgcatatc ctgaaaatgg tattgatgca
     3301 atagttacag cttcacaaat tgttacatca ttacaaacta taataagtag aaacttagaa
     3361 ccaaataatg ctgccgtttt gactataggt aaaatatatg gtggtgatgc acacaatgta
     3421 atatgtgaag atgtaaaact agaaggcact ttaagaacct taaatagcaa aacaagaaac
     3481 tttatgattg ataaaatagc taaaattgtt ggagacactg catctgcatt tggttgtgtt
     3541 gggactttac atgtaagtga tgaaaactat cctgctgtta taaatgaaaa agaattagta
     3601 gatacagtta tatcaagtac taaagaatta cttggagagg aaaaatttat attgagacca
     3661 aacccgtctt taggtggtga agacttttct ttttacacag aacattgtaa aggagcattc
     3721 ttccatttag gatgtaaaaa tgaggaaaag ggcttaattt cacctcttca tacctctagc
     3781 ttcaatatag atgaagattg tttaccaata ggtgttatga tgcatgtaat gaatactttg
     3841 tattttaatt agtacagtaa ttaataaaag aataggagat aaattaatat ggaaaaagct
     3901 aagaaattag gtctatttag tatgatatta ttagggatta atagcataat aggttcaggt
     3961 atattcttac taccaggtaa agtttatagt ctagcaggtc aaaacagtat gtttatatac
     4021 atttttgcta cactcttagt tctatcaata cttttatgtt ttgcagaagt tggaagcatg
     4081 tttgataaga atggaggagc atatctatac tccaaaaaag cttttggaga ttttatagga
     4141 tttgaagttg gtacaatgtc ttggtttatt agaattatat catggtctac tctagcagta
     4201 ggatttgcaa cagctttagg ttcattttgg ccagaatctg ccactgaata caaaggttgt
     4261 atagctgcaa tactcgtaac actactttca ataaatagct tatttggaat taaaagcaca
     4321 aagataatga ataatgtaat aactatagct aaattagtac cattaatagt ttttgtgata
     4381 gttggaatat tctttataaa atttgtaaat attgttcctt caggaaatgt agttaactct
     4441 agtatgggtc cagccattat attagtattt tacgcattta ccggctttga atcatttatt
     4501 gtggcaagtg gagaaatgga aaatcctaaa aaaaatcttc caatcgcatt aataaccaca
     4561 atatttatat gtgcaataat ttatatatta attcaaattg tctgtatggg tatacttgga
     4621 gatagactat ttgaaaacag tattccaatt gcagatgctt caagtgtatt tttaggcaat
     4681 tatggtaaag ttttcatatc tgtagcaaca ttaatatcta tatttggtat aaatataggt
     4741 tcatcaatag taactcctaa atgtggttct tctctagcag aagaaggttc tcttcctgca
     4801 tttataggaa aaacaaataa atatggtgct ccatatgtag caataataat atctttaatt
     4861 tgttgtatac cacttgtgct aactggaagt tttgaacaac ttgcagtaat gagtgtaatt
     4921 gcaagatttg ctcagtacat accaacttgt ttatcagtaa tagttttaag aaaaagaaca
     4981 gatgttaagg cctcttttaa gattccattt ggtcctgtta taccaatggt agctatttta
     5041 ggtagcttat ggttgttaca acaagcttgg gcagaagata tttcaaaacc tataactcaa
     5101 aatagagtct taataggatt aggggcaatg ttgttaatag ctcctctata tatatttatg
     5161 aaaaaaaata aggaaatcga gcataaaaat aaatcagaag attctaaatt atatatttcg
     5221 agagaaaaaa ttcaataata tttt
//
LOCUS       DAMJWP010000235         5130 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.242, whole genome shotgun sequence.
ACCESSION   DAMJWP010000235 DAMJWP010000000
VERSION     DAMJWP010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5130
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.242"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            230..1309
                     /locus_tag="PC700_003214"
     CDS             230..1309
                     /locus_tag="PC700_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDF2582484.1"
                     /translation="MPELLKNMYNRESLYEVAVAIQSVYNSFKVDEFIKSTMDETWNN
                     LELKARCRKISMSLGMYLPEDYKEALSILEKSVTGFYFAFFFPDFVEVYGQDEINWDL
                     SISALERNTEYWSSEFAVRAFIIKDEERMMAQMRKWSKHKSEHVRRLASEGCRPQLPW
                     GQAISKFKKDPTPVLPILEQLKTDTSTYVQKSVANNLNDISKTHPDLVISIAKDWYGK
                     NKSTNWIVKHGCRTLLKKGNRDVLALFGYDDTTSINLQDFTLETTSISIGEDLTFSFN
                     ILAKKATKTRLEYGIDYMKSNGKRNRKIFKISEVSLKENEKKSYMKKHSFADVSVRRH
                     YPGIHSITIIINGVEKGKLDFELGV"
     gene            1633..2001
                     /locus_tag="PC700_003215"
     CDS             1633..2001
                     /locus_tag="PC700_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HDF2582485.1"
                     /translation="MDVYSFWNDTLAQDAVKIRKYFHEDAYINWHDTNEHFTLEEFII
                     ANCEYPDKWNGKVERVLEFGNLVITVTHVYAINKPLSFHVVSFISLKDDKIISIDEYW
                     GEDDDVPQWRLDKHIGKPIK"
     gene            2118..2921
                     /locus_tag="PC700_003216"
     CDS             2118..2921
                     /locus_tag="PC700_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2582486.1"
                     /translation="MEEKYILSDRGKVYYWIKESNSVFAKNLIFLPGLTANHHLFDFQ
                     IQYFSKNHNVLVWDAPAHGKSRPYLDFSYSNLVEELKVILDKQNMKKVVLIGQSAGGF
                     VAQSFIVKYPNMVEGLMMIGTCPYGTDYYSKSDIFWLKQTEWMLKLFPDKLLRNIISK
                     MCGATSEGRRSMMKMLEGYEKKELCHLMYLGFAGFMPEIQDLNIHCPVCLVVGDKDKT
                     GKVRKYNEQWHNKTGYPLHIIKNASHNVNVDKPSELNQIMDIFIKQLNI"
     gene            3306..4196
                     /locus_tag="PC700_003217"
     CDS             3306..4196
                     /locus_tag="PC700_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2582487.1"
                     /translation="MIESIPAKQILQKVKFDNTRWFGIDYNMNLYRGCSHGCIYCDSR
                     STIYNIENFDKVRYKENVIEILSKELRSKRKKGVVGIGAMSDTYNPFEKQLCITKQAL
                     DLISENHFGVSIDTKSSLVVRDIPILQKIKKNNSAIVKLTITTANDELSKKIEPYVNP
                     SSARFDAVKELNDSGIFCGILLTPMLPFLTDNKDEIRAIVEKAHKANAKFIYCMYGVT
                     MRSGQREFFYEHLRDISPKLVLKYQKTYGLNYVCTIQNKDSCEKLLREECARYGILTD
                     MKEIIKAYKRSESYIQIKFF"
     gene            4659..5102
                     /locus_tag="PC700_003218"
     CDS             4659..5102
                     /locus_tag="PC700_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2582488.1"
                     /translation="MIEIKTIEINKKSQIDLLMLADPCEEMIDKYLNKGTMYALYDNK
                     ELTCIAVVNEISKEICELKNIATYEHFQNMGYASKMIYHLLDVYSKKYSSMVVGTSES
                     GVPFYEKFGFVYSHKIKNFFVDNYPEPIFEGELQCVDMLYLSYNF"
BASE COUNT         1957 a          550 c          955 g         1668 t
ORIGIN      
        1 agtaaagtat aaaattctat tactataata aaagatagaa ctagaagtct ttatgaaagt
       61 gggaaaagta tttatataaa taaatttata tggtttatag tttaacagta ggaaatatcg
      121 tatgagtgag aatatcgtat tttacataaa gatttagcaa tatgacacac tgttgtcgtg
      181 ttctgtagga tatgattaat aaatcagata tgaaaggaga gtgagtaata tgccagaatt
      241 actaaaaaat atgtataatc gtgagtcact ttatgaagtt gcagtagcta tccaatcagt
      301 ttacaactca tttaaggtag acgagttcat aaaatctaca atggatgaga catggaataa
      361 cttagaatta aaagccagat gtcgaaagat aagtatgagt ttaggaatgt atttaccaga
      421 agactataag gaagctcttt ccattcttga aaaatcagtt acaggttttt attttgcttt
      481 tttctttcca gattttgttg aagtttatgg gcaggacgaa ataaattggg atttatctat
      541 aagtgcatta gaaagaaata ctgagtattg gtcttcagaa tttgctgtaa gagcatttat
      601 catcaaggat gaagagcgta tgatggcaca gatgcgtaaa tggtctaagc ataagagcga
      661 acatgtacga agacttgcaa gtgaaggttg tcgtccccag ttgccttggg gacaagcaat
      721 atccaaattt aaaaaagacc ctacacctgt actgcctata ttggagcaac ttaaaacaga
      781 tacatcaacg tatgtacaaa agagtgttgc taataatcta aatgatatct caaaaacaca
      841 tccagattta gtcataagca ttgcaaagga ttggtacggt aaaaataaat ctactaactg
      901 gattgtaaag catggttgta gaactctact aaaaaaaggc aatcgtgatg tattagccct
      961 ttttggatat gatgatacta cttctataaa tcttcaggac tttactttag aaacaacgtc
     1021 tatttctatt ggagaggatt taactttttc atttaatatt ttagcaaaaa aagcgacaaa
     1081 aacaaggctc gagtacggaa ttgattatat gaaatctaat ggaaaaagga ataggaagat
     1141 atttaaaata tcggaggttt cattaaaaga aaatgagaaa aagtcatata tgaaaaaaca
     1201 ttcatttgca gatgtaagtg taaggagaca ttatccaggt atccactcga ttactattat
     1261 tattaatggg gtagaaaaag gcaaacttga ttttgaatta ggtgtataat tttactaaca
     1321 gcataaccta tatcagatac tttgctaaac ctatatcgta tactctaata aatctatatt
     1381 aggtagttta ctacttgttt actgaattta aatacaaaga aatacaggtg ttaatccctg
     1441 cccattaaag gaatatgagc ataacacaaa agtagaactt atcaattctg ttacgggtat
     1501 agtagtaaat ccatagaatc acaattagaa gatgaagtgt aaaaaataag ttagtgttga
     1561 ttttagttgc tagtgatatt gaatagttat acaatagcca tataaattag aatttgtaga
     1621 ggagaaatgt aaatggatgt atatagtttt tggaatgaca ctttggcaca agatgctgtt
     1681 aagataagaa aatattttca tgaagatgct tatattaatt ggcatgatac aaatgagcat
     1741 tttactttag aagaatttat tattgcaaat tgtgagtatc ctgataaatg gaatggtaaa
     1801 gtagaaaggg tgctagaatt tggaaattta gtaattacag tgacacatgt ttatgcaata
     1861 aataaaccac tatcatttca tgtcgtttca tttatcagcc ttaaagatga taagattatt
     1921 tctattgatg aatattgggg agaggatgat gatgtgccac agtggagatt agataaacat
     1981 attggaaagc caataaaata gtgtttgttc tgttttataa ctgaaatttt gataacattt
     2041 gatattttga tttgtcattg gtcaacatag tcgttagtgt aataatttaa aaaagtagaa
     2101 tttggagagt gagcactatg gaagaaaaat acatcttatc tgaccgaggt aaagtatatt
     2161 attggataaa agaaagtaat agtgtgtttg caaagaactt aatttttttg ccaggactaa
     2221 cagcaaatca tcatcttttt gatttccaga tacagtactt ttcaaaaaat cataatgtat
     2281 tggtatggga cgctcctgct catggaaaat caagacctta cttagatttc tcatattcta
     2341 acttggtaga agaattgaaa gtaattttag ataaacaaaa tatgaaaaag gttgtactta
     2401 tagggcaatc ggcaggggga tttgtggcac agtcctttat tgtaaaatat ccaaatatgg
     2461 tagaaggatt aatgatgatt ggaacttgtc catatggaac tgactattat agtaagtcag
     2521 atattttctg gttaaaacag actgaatgga tgttaaagtt attcccagat aaattgttgc
     2581 gaaacataat atctaagatg tgtggagcta cgtctgaagg cagaaggagt atgatgaaaa
     2641 tgttagaagg ctacgaaaaa aaagaactgt gtcatctcat gtatctggga tttgctgggt
     2701 tcatgccaga aattcaagac ttaaatatac attgtccagt ttgtttggtg gttggagata
     2761 aagacaagac gggtaaagtg agaaaatata atgagcagtg gcacaataag actgggtatc
     2821 cattgcacat tataaaaaat gcttcacata atgtaaatgt cgataaacct agtgaactaa
     2881 atcagattat ggatatattt attaagcaat taaatatata aaaactacat actcattagg
     2941 taaattatgt agtatatgtg tttacatatg gttatagaaa taaatatgta ataaactact
     3001 taacacttaa aaagtggatt tgtccagagc ataatacaaa gcatgatata taaattttta
     3061 agcgaaatac atcaaaagaa gtattagaat tagtggagat aaaacagcta ttagagatta
     3121 taaataaata gttttaaagg tactacgaat atagaggaag gtaaatcata cgttattcaa
     3181 ggattataaa tgagtagttt aaaagtacta tcggataggt ctctgcatat ttaaacctta
     3241 gtaaaggtag ttaaagtagt agtttaaaaa ctaaaaaatt atataaagga attaggtata
     3301 agaaaatgat tgagagtata ccagcaaaac aaattttaca aaaagtgaaa tttgacaata
     3361 caagatggtt tggaattgac tataatatga atctctatcg tggttgttct catggatgta
     3421 tatattgtga tagccgtagc acaatataca acatagagaa ttttgacaaa gttagatata
     3481 aagaaaatgt gatagagatt ttaagtaaag aattgcgttc aaaaagaaaa aaaggggtag
     3541 taggcattgg agctatgtct gacacatata atccttttga aaagcaactt tgtattacaa
     3601 agcaagcact agatttgatt tcggaaaatc attttggagt gtctattgat actaaaagta
     3661 gtttggtggt tagagatata ccgattttgc agaaaattaa aaaaaataat tctgctattg
     3721 taaagttgac tataaccact gcaaatgatg aattatcaaa gaaaatagaa ccttatgtaa
     3781 atccaagttc tgcacgattt gacgcagtga aagaacttaa tgattctgga attttttgtg
     3841 gcatattatt gactcctatg cttccatttt tgactgataa taaagatgaa atacgagcaa
     3901 ttgtagaaaa agcacacaag gctaatgcaa agtttattta ttgcatgtat ggtgtaacaa
     3961 tgcgtagtgg tcagagagag tttttttatg agcatttaag agatatatca ccaaaattag
     4021 tacttaaata tcagaaaaca tatggtttaa actatgtatg tactatacaa aataaagatt
     4081 cttgtgagaa gttgttaaga gaagaatgtg cacgttatgg tattttgaca gatatgaaag
     4141 agattataaa agcgtacaag aggtcggagt catatattca aataaaattt ttttaagata
     4201 ggctttggat gagatataat agtggtgatt gatgtgttga tattatagtt gattgttaga
     4261 gaggatatta tagatgaata ttaatattga tattctaggt aattgctaga actaatatta
     4321 tagatgaata ttagtattga tattctaggt aattgctaga gttagtatta tagatgaata
     4381 ttagtattga tattctaggt aactgctaga gctaatatta tagatgaata ttagtattga
     4441 tattctaggt aattgctaga gctgatatta tagatgaata ttagtattga tattctaggt
     4501 aattgctaga gctgatatta taatttatta ttaatattaa tattctaggg aattatcact
     4561 atattttaaa aagtaaaaat atatatcaaa atgaactaaa taagtactgt aagaagtttg
     4621 ttaaattaag gtttgaataa agtgagggtg gagaacttat gatagaaata aaaactatag
     4681 aaataaataa aaaaagtcaa attgaccttt taatgttagc cgacccatgt gaagaaatga
     4741 ttgataaata tctcaataaa ggaactatgt acgcattgta tgataataaa gaacttactt
     4801 gtatagcagt tgtaaatgaa atttccaaag aaatatgtga attaaaaaac attgctacat
     4861 atgagcattt tcaaaatatg ggttatgcct caaagatgat ttatcatctt ttagatgttt
     4921 atagtaaaaa atattcatca atggttgttg gtacatctga aagcggagtt cctttttatg
     4981 agaagtttgg atttgtttat tcacataaaa ttaaaaactt ttttgtagat aattatccag
     5041 aaccaatctt tgaaggagaa ttacaatgtg ttgacatgtt gtatctatca tataactttt
     5101 agataggata atatttttag gtagatataa
//
LOCUS       DAMJWP010000236         5110 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.243, whole genome shotgun sequence.
ACCESSION   DAMJWP010000236 DAMJWP010000000
VERSION     DAMJWP010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5110
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.243"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            147..1040
                     /locus_tag="PC700_003219"
     CDS             147..1040
                     /locus_tag="PC700_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDF2582489.1"
                     /translation="MKNKKFIVIILIISILIGVLVKEYSSREIKKDDINVSKYIKYAD
                     LASKNNAQVNWKYVASIVAVLNKNNLKNVKDSQIQEVSDLFVKNFSKNNKINKLSDIL
                     DELEFSNRQKRLVDNYIDNLKDYGIKPERLKSDTKYMKFIAEIKTEAIQNYKDYKILP
                     SITIAQAIIESSWGKSTLAKQYNNLFGIKADAYWKGKSVTLETKEHLDTIIDDKFRIY
                     DDKNESIKDHAKFLATNKRYKNNGVFDAKTYIYQAKALEKAGYSTAKDENGNSIYAAR
                     LIELIQQYNLQLIDSEIQSEV"
     gene            1088..3388
                     /locus_tag="PC700_003220"
     CDS             1088..3388
                     /locus_tag="PC700_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582490.1"
                     /translation="MKKIFSIFISCMVVVTTITGCSSGEVIKNTSPINSVKNLDFSFE
                     VKPEIFEVSVESGGSKEKISEPSKERKVSNFKKNKTEASWTYPDENIDINIKKEDSYL
                     DVNIKSNLEKETSFNWPKVEGESYILPLYEGKFIPSNDKYWKEYLNESTYNVIESFSM
                     QFFAVNKAKYSVIYIIENEFNNVIEFSSKDKINFTFKHEYPSINKNKEYGFKIYLTNK
                     NITDVAKTYKNYIVDKGEFKTLKEKSKENKNIEKLYGAPHIYFGDKSIITINDIEWNK
                     LKENLSDDLTNWMKELLSMKSGNSEFIKVINQIKTQDYIDKYQKNQIVQAFNEVMQMN
                     TFYNEKVFESKDREIKKVLDKGISKLSRVELIEFNKKLLKSKLEESVNDVATWAKYNT
                     TDVINDINKSGIKNAWIGFDDMEEGYISLEMVKRANELGYLVGPYDSYHSIHKPGEEK
                     WSTASFKDKSLYENATIENKKGEKETGFQGEGRKLNPTLSLPSVKERVQSILDEGYEF
                     NSWFIDCDATGEIYDDYSQNHITTQTQDLNARLERMSYIRDHANMVIGSEGGNDFSSS
                     TIAFAHGIETPAFAWVDKDMSKNKGSEYYVGKYYSPTGGVPEIFSKQIPVKERYKKIF
                     IDPIYSLPLFRLVYNDSVISSHQWLWGTLKIKDEVGSRMMKEVLYNTPPMYYIDRTEW
                     EKHKDEIISNNKVWSAFNKKAIKKEMTDFKFISEDRLVQMTEYGEDLKVLVNFSQKDI
                     KYNDEVIKANSLVIYDADSKVIYKPE"
     gene            3622..4113
                     /locus_tag="PC700_003221"
     CDS             3622..4113
                     /locus_tag="PC700_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HDF2582491.1"
                     /translation="MYENNKVIYNFERCEKLENALYLSQFNTYKNFKLTSKKTVINSL
                     DTSTYNMNIHSISLVDTMECISSEDEQMTGKKLILVGDIDTDVTLDYAGNKRNRNSSK
                     KNTFKLKIPFSTFIQMPRKIENKDKINLKYLIQDITSSILDDNLFISVTAIISYENSS
                     KIE"
     gene            4141..5088
                     /locus_tag="PC700_003222"
     CDS             4141..5088
                     /locus_tag="PC700_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582492.1"
                     /translation="MRITKTTFNDVFSNDANGSIIKVEKYVDKKCASVFEILTYTIIV
                     TNTSRYKTGNIFFKDYISKYIEFINNTVKVNGIIKRGLDPQQGFYIGRIDASCKKIIS
                     FKSVVLPNSAHRIIKNSANIYYYYKCNLDKFPTRISIESNRVYTNVNKIVFKQLNISN
                     TLKAPKNLRDILKVNVNSKIFDVKPMKNLINKGSNLKLCNSIVFGCIEVEIDYSCKNR
                     SNFKTVNKFNDIKKDKYYKNDIQNNNENINDCKKQISFGDNKVKKITKTFGFSCFICS
                     PVGIIYEDMKNINIKIEHTSINELNPEELFVNTSLLLYY"
BASE COUNT         2196 a          467 c          833 g         1614 t
ORIGIN      
        1 agaaaactat atattataac aatagtagtg cctttaatta aatatatttc ataaatcttc
       61 ataaaatctt caaaatttcg taaagaatat gtcacaaaag taaggtatat tatattcagc
      121 agatagtata agggaggaca atatttatga agaataaaaa attcattgtt ataatactga
      181 taatatcaat tttaataggt gtattagtga aggaatattc atctagagag ataaaaaagg
      241 atgatataaa tgtcagtaag tatataaaat atgctgattt agcaagtaaa aataatgcac
      301 aagttaactg gaagtatgta gcttccattg ttgctgtgtt aaataaaaat aacttaaaaa
      361 acgttaagga ttcacagata caagaagtat cagatttatt tgttaagaat ttttctaaaa
      421 ataataaaat aaataagtta agtgatattt tggatgagtt agaatttagt aatagacaaa
      481 aaagattggt tgataattat atagacaatc ttaaggatta tggtataaaa ccagaaagat
      541 tgaagtctga tacaaaatat atgaagttta ttgctgaaat taaaacagaa gcaatacaaa
      601 attataagga ttataaaata cttccatcta taactattgc acaagctata atagaatcta
      661 gctggggcaa gtcaactttg gcaaaacaat ataacaatct atttggtata aaggctgacg
      721 cttattggaa aggtaagtca gtgactttag aaacgaaaga acatttagat acaattatag
      781 atgataaatt tagaatatat gatgataaaa atgaatctat aaaagaccat gcaaaatttt
      841 tagccacaaa taaaaggtat aaaaacaatg gtgtatttga tgcaaaaacg tatatatatc
      901 aagctaaagc attagaaaag gcaggttata gtactgcgaa ggatgaaaat ggcaatagta
      961 tatatgcagc tcgtctaata gaattaattc aacagtataa tcttcaatta atagacagcg
     1021 aaattcaaag tgaagtgtaa cttttatgta acataggagt gatatattta tcgaggaggt
     1081 ggtttttatg aaaaaaatat tctcgatatt tataagttgt atggttgttg taactactat
     1141 tactggatgt agtagtggtg aggtgataaa aaatacatca cctatcaata gtgttaaaaa
     1201 tttggatttt agttttgagg tgaaacctga aatatttgag gtatcagttg aatctggtgg
     1261 ttcaaaagaa aagatatctg aaccctcaaa agaaaggaaa gtatcaaatt ttaaaaagaa
     1321 taagacagag gcatcgtgga cgtatcctga tgaaaatata gacataaata taaaaaaaga
     1381 ggacagctat ttagatgtaa atattaaatc taatttagaa aaagaaacta gttttaattg
     1441 gcccaaagtt gaaggggaaa gttatatact accactttat gagggtaagt ttataccaag
     1501 taatgataag tattggaagg agtatctaaa tgagtcaact tataatgtta tagaatcttt
     1561 ttcaatgcaa ttctttgcag taaacaaggc aaagtattct gtgatttata ttatcgaaaa
     1621 tgaatttaac aatgtaattg aatttagttc taaagacaag attaatttta catttaaaca
     1681 tgaatatcca agtatcaata aaaataagga gtatgggttt aaaatatatt tgactaataa
     1741 gaatattaca gatgtagcaa aaacttataa aaattatata gttgataaag gagaatttaa
     1801 aacattaaaa gaaaaatcta aggaaaataa aaatattgaa aaactttatg gagcaccaca
     1861 tatttatttt ggagataagt ctatcatcac aataaatgat attgaatgga ataaattaaa
     1921 agaaaattta tcagatgatt tgactaattg gatgaaagag cttttaagta tgaagtctgg
     1981 caatagtgaa tttataaaag taattaatca aataaaaact caagattata tagataaata
     2041 tcaaaaaaat caaatagtac aagcatttaa tgaagttatg cagatgaata ctttttataa
     2101 tgaaaaagtt tttgaaagca aagatagaga aataaaaaaa gtattagata aaggtatatc
     2161 taaattaagt agggtagaac ttatagagtt taataagaaa ttattgaaat caaaattgga
     2221 agagagcgtg aatgatgttg ctacatgggc aaaatataat acaactgatg taattaatga
     2281 tattaataag tctggtatta aaaatgcgtg gataggattt gatgatatgg aggaaggtta
     2341 tatatcctta gagatggtta aaagagctaa tgagttaggt tatctcgtag ggccatatga
     2401 ttcataccac tctatacaca aaccagggga agaaaagtgg agtacagcat catttaaaga
     2461 taaatctcta tatgaaaatg ctaccataga aaataaaaaa ggtgaaaaag aaactggatt
     2521 tcaaggagaa ggaagaaaac taaatcctac attgtcttta ccaagtgtaa aagagagagt
     2581 acaaagtata ttggatgaag gatatgaatt taattcttgg tttatagatt gtgatgctac
     2641 aggagaaatc tatgatgatt attctcaaaa tcatataaca acacaaacac aagatttaaa
     2701 tgctagactt gaaagaatgt catatataag agaccatgca aatatggtca taggttcaga
     2761 aggtggaaat gatttttcaa gtagtactat agcatttgca catggtattg agacaccagc
     2821 atttgcttgg gtagataaag atatgagtaa gaataaaggc agtgaatatt atgttggtaa
     2881 atactattct ccaacaggag gagtaccaga aatattttca aagcagatac ctgtaaaaga
     2941 aaggtacaaa aaaatattta tagacccaat ttactctcta cctttattta gattagttta
     3001 taatgattct gtgatatctt cccatcagtg gttatgggga actttgaaga ttaaggatga
     3061 agttggaagt agaatgatga aagaagtatt atataataca ccacctatgt attatataga
     3121 tagaactgaa tgggaaaaac ataaagatga aataatctca aacaataaag tatggtcagc
     3181 gtttaataag aaagctatta aaaaagaaat gacagacttt aaatttattt ctgaggatag
     3241 actagttcaa atgactgaat atggagaaga tttaaaggta ttagttaatt tttctcaaaa
     3301 agacattaag tacaatgatg aagtcataaa agcaaattct ttggtaatat atgatgctga
     3361 tagtaaagtc atatataaac cggaataata gatttaatga aaataaagat tgtttaaatt
     3421 tcaagtaaaa aataatataa tattcattta gtttgtatta aagtagtgac tatattaatc
     3481 tggataatta gtatagtcac tattatttat attctttcct aaaagtaagt agtaactgaa
     3541 tcgtgaataa tcaaaaatgg taatttgaaa tagtttttat gaatagtatt aatatataga
     3601 tatagggaga ttgatgtgat tatgtatgaa aataataagg taatatataa ttttgaaaga
     3661 tgtgaaaagc tagaaaatgc tctttattta tctcaattta atacatataa aaactttaag
     3721 ttaacttcta aaaaaactgt aataaactca ttagatacat ctacttataa tatgaatatt
     3781 catagcataa gtctcgttga tactatggaa tgtatatcgt cagaagatga acaaatgaca
     3841 ggaaagaagt tgattttagt tggcgatata gatactgatg taacattaga ttatgcaggc
     3901 aataaaagaa atagaaattc tagtaaaaaa aatacattta aattaaaaat accctttagt
     3961 acatttatac aaatgcctag aaagatagaa aataaagata aaattaattt aaagtatctt
     4021 atacaagata ttacttcatc tattttagat gataatttat ttattagtgt tactgcaatt
     4081 ataagttatg aaaatagttc caagatagag taatctttaa attatagaaa ggtggatgca
     4141 atgagaataa cgaaaactac ttttaatgat gtattttcaa atgatgcaaa tggaagtatt
     4201 ataaaagtag aaaaatatgt tgataaaaaa tgtgcatctg tatttgagat tttgacctac
     4261 actattattg ttacaaatac aagtaggtac aaaactggaa atatattttt caaagattat
     4321 atttctaaat atatagagtt tataaataat actgtaaaag ttaatggcat tataaaacgt
     4381 ggattagacc cacagcaagg tttttacata gggagaatag atgcaagttg taaaaaaata
     4441 atatctttta agagtgtagt tttaccaaat tctgcacata ggattataaa gaatagtgct
     4501 aatatatact attattataa gtgtaatcta gataaattcc caacaagaat atcaatagaa
     4561 agtaatagag tttatactaa tgtaaataaa attgtattca aacaattgaa tataagtaat
     4621 actttaaaag ctcctaaaaa tctaagagat atattaaaag taaatgtaaa tagtaaaata
     4681 tttgatgtta aacctatgaa aaatcttata aacaaaggta gtaatttgaa attatgtaac
     4741 tcgatagttt ttgggtgtat agaagttgaa atagactata gttgtaaaaa taggagtaat
     4801 tttaaaacag taaataagtt taatgatatt aaaaaagata agtattataa aaatgatata
     4861 caaaataaca atgaaaatat aaacgattgt aaaaagcaaa taagttttgg tgataataag
     4921 gtaaaaaaaa taacaaaaac ttttggattt tcttgtttca tttgttctcc agttggaatt
     4981 atttatgaag atatgaaaaa tataaatata aaaattgaac atacatcaat aaatgaatta
     5041 aatccagaag aattatttgt aaatacatca cttttattgt attattaagt attttaaagc
     5101 taaaacttaa
//
LOCUS       DAMJWP010000237         5081 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.244, whole genome shotgun sequence.
ACCESSION   DAMJWP010000237 DAMJWP010000000
VERSION     DAMJWP010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5081
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.244"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            122..760
                     /locus_tag="PC700_003223"
     CDS             122..760
                     /locus_tag="PC700_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2582493.1"
                     /translation="MIIIQLNERQLKIIDIVKENEPITSESIASSLNVTRATLRSDLA
                     ILTMTGILDARPKVGYFYSGVSEINLIGKSVKEKTVEDIMSMPVLAKKDESIYDVIVT
                     MFLSDVGSIVIIDENEELCGVVSRKDLLKATIGGSDINKMPIGMIMTRTPNVVTLTKG
                     ASVLLASRKIIEHEVDSIPIVEYKGEDKNHMRVVGRISKTNITKLFLEIVDN"
     gene            767..1609
                     /locus_tag="PC700_003224"
     CDS             767..1609
                     /locus_tag="PC700_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HDF2582494.1"
                     /translation="MLVKNLIIYAVSDSVGETAQQVAKACMSQFYVNETYEIKRFPYM
                     INKGVLLETLENAKAENALIVYTLVDEELCSIVERYCEREGLSCIDLMTDILREISKR
                     TGRKPKREAGIIRKLDESYFKRVEAIEFAVKYDDGKDPRGVLQADIILVGISRTSKTP
                     LSMYLANKNIKVANVPLVPEIPIPKEVFEIDTKKIIGLTNSPEKLNEIRTQRLKALGL
                     SSKANYANLERILQELDYSEEIMKRIGCPVINVSNKAIEETAGIILDIMKENGLKIYK
                     EIEI"
     gene            1670..4297
                     /locus_tag="PC700_003225"
     CDS             1670..4297
                     /locus_tag="PC700_003225"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HDF2582495.1"
                     /translation="METKYVYSFGEGSKDMKSLLGGKGANLAEMTKIGLPVPPGFTIT
                     TEACNDYYVNNESIRAEIIKEIEEHLATLEKDLNKTLGCNKNPLLVSVRSGAVFSMPG
                     MMDTILNLGLNDNSVVGLAEATQNERFAYDSYRRFIQMFSDVAMEVPKYKFENVLDRV
                     KEAKGYTVDTELTTDDLKEIVKEFKAIYKKEIKNDFPQDPKEQLMLAIEAVFRSWNNP
                     RAIVYRKLNDIAHNLGTAVNIQSMVFGNMGETSGTGVAFTRNPATGENKLFGEFLMNA
                     QGEDVVAGIRTPQNISTLADVMPAVFDEFVKITHILEGHYKDMQDIEFTIENERLYIL
                     QTRNGKRTAAAAINVAVDLVEAGIIDEKEAIMRIEPNQLDQLLHPKFEDKALKEAKVI
                     AKGLPASPGAASGKVYFNADDVVKANEKGEKVVLVRLETSPEDIEGMVKAEGILTARG
                     GMTSHAAVVARGMGKCCVAGCGEIKVDEFNKEIRALDDVVIKEGEYISIDGSTGNVYL
                     GDVKKTEVSLTGNFEKLMNWVDKHKCMMVRTNADNPRDARAAIEFGAEGIGLCRTEHM
                     FFDEARLPAVREMILSNTVEQREKALEKILPMQREDFVELFKVMDGKPVNIRLLDPPL
                     HEFLPHDDETIEELSKSMGIKVSDIKKRIVDLDEFNPMLGHRGCRLAITYPEICVMQS
                     KAIIQGAIEAIKAGVKVSPEIMVPLVGEVNELKIIRKMIVETVDAIIKEEGIEVPYTV
                     GTMIEIPRACLTADEIAQEADFFSFGTNDLTQMAFGYSRDDAGKFLGQYVDEEILEKD
                     PFQVLDQNGVGKLVKMGAKLGREVKPELKLGICGEHGGEPSSVEFCYSVGLNYVSCSP
                     FRVPIARLAAAQASIKNPR"
     gene            4511..4756
                     /locus_tag="PC700_003226"
     CDS             4511..4756
                     /locus_tag="PC700_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2582496.1"
                     /translation="MTKCPKCGKEVSSRPGSICPRCGFKVADESLKIRCPEPSCRALV
                     SRKLDYCPKCGCKLRGYNISEFVNMVRCKIDETFDKK"
     gene            4968..>5081
                     /locus_tag="PC700_003227"
     CDS             4968..>5081
                     /locus_tag="PC700_003227"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2582497.1"
                     /translation="MSKKHKKYSKELKLKAVNLYIKEGYSSYKIAEMLNIRS"
BASE COUNT         2043 a          575 c         1002 g         1461 t
ORIGIN      
        1 gagaacttag taaaaaatat atcgtattca ataaaaaatc tgtttacaga atggtatata
       61 tagttatata atatactata taagatgcga aaagataaaa agtatagcac aatgaaagaa
      121 ggtgattatt attcagctta atgaaagaca actaaaaatt atagatatag ttaaggaaaa
      181 tgaaccaata acgagtgaaa gtattgcctc cagcttgaat gtgactcgtg ctacacttag
      241 atctgattta gctatattaa caatgacagg aatattagac gcaagaccaa aagttggata
      301 tttttattca ggagttagtg aaataaattt aattggtaaa agtgtaaaag aaaaaacagt
      361 agaggatata atgagcatgc ctgtactagc taagaaggat gaaagtatat atgatgtaat
      421 agtcactatg tttttatctg atgtagggag tatagttatt atagatgaga atgaagagtt
      481 atgtggagtt gtttccagaa aagacttgct aaaagcaact ataggaggtt ctgatataaa
      541 taaaatgcca ataggtatga taatgacaag aactccaaat gtagtcactt taactaaagg
      601 agcaagtgtt ttattagctt caagaaaaat aatagaacat gaagtagatt cgattccgat
      661 tgttgaatat aagggagaag acaaaaatca tatgagagtt gttgggagaa tatcaaaaac
      721 caacataact aagctattct tagaaatagt tgacaattaa ggaggtatgc tggtgaaaaa
      781 cctaattata tatgcagtat cagattcagt aggggagact gcacaacaag ttgcaaaggc
      841 atgtatgtct cagttttatg tgaatgaaac ttatgaaata aaaagatttc catacatgat
      901 taataaagga gtactacttg aaactttgga gaatgcaaaa gctgaaaacg ctttaattgt
      961 atatacatta gttgatgaag aattatgcag tattgttgaa agatattgcg aaagagaagg
     1021 tcttagttgt atagatttaa tgacagatat actaagagaa ataagcaaga gaacaggaag
     1081 aaaacctaaa agagaagctg gtataattag aaaattagat gaatcttact ttaagagagt
     1141 tgaagctata gaatttgcag tgaaatatga tgatggaaaa gaccctagag gtgttcttca
     1201 agcagacata attttagtgg gaatatcaag aacatctaaa actcctttga gtatgtattt
     1261 agctaataaa aatataaaag tagctaatgt tccattagtt ccagaaatac caattcctaa
     1321 agaagtgttt gagatagata ctaaaaagat aataggtctt actaattcac ctgaaaagct
     1381 gaatgagata agaacccaaa gattaaaagc tttaggattg tccagtaaag caaactacgc
     1441 taatttagaa agaatacttc aggagttaga ttattcagaa gagataatga aaagaattgg
     1501 atgcccagta ataaacgttt caaataaggc aatagaagaa acagcaggta taattttaga
     1561 cataatgaaa gaaaatggtc taaagatata caaagaaata gaaatttaat aatgtctatt
     1621 aataaaaatg atataaatta aaataaaaag attaatgggg gatttaacaa tggaaactaa
     1681 gtatgtttat agttttggtg aaggaagcaa agacatgaaa tctttactag gaggtaaagg
     1741 tgccaattta gcagaaatga ctaagatagg cttacctgtc cctcctggat tcactataac
     1801 aacagaagca tgtaatgatt attatgttaa caatgaaagt ataagagcag agataataaa
     1861 agaaatagaa gagcatttag ctactttaga aaaagactta aataaaacat taggatgcaa
     1921 taagaatcca ttattagtat cagttcgttc tggtgcagta ttctctatgc caggaatgat
     1981 ggatacaatt cttaacctag gtcttaatga caatagtgtt gttggcttag cagaggctac
     2041 acaaaatgaa agatttgcat atgatagtta cagaagattc atacaaatgt tttctgatgt
     2101 tgctatggaa gttccaaaat ataaatttga aaatgtatta gacagagtaa aagaagcaaa
     2161 gggatacaca gttgatacag aacttacaac tgatgattta aaagaaatag taaaagaatt
     2221 taaagcaatt tataaaaaag aaataaaaaa tgatttccca caagacccaa aagagcaatt
     2281 aatgcttgca atagaggctg tatttagatc atggaataat cctcgtgcaa tcgtataccg
     2341 taaattgaat gatatagctc ataacttagg aacagcagta aatattcaat caatggtatt
     2401 tggtaatatg ggagaaacta gtggtacagg tgttgcattt actagaaatc cagctacagg
     2461 agaaaataaa ttatttggtg aatttttaat gaatgcacaa ggtgaagacg ttgttgcagg
     2521 tataagaact cctcaaaata tatcaactct tgcagatgta atgccagctg tattcgatga
     2581 atttgtaaaa attactcata tacttgaagg tcattataaa gatatgcaag atatagagtt
     2641 tactatagaa aatgaaagat tatatatact acaaactaga aatggtaaga gaacagcagc
     2701 agcagcaata aatgtagcgg ttgatttagt tgaagctgga ataattgatg aaaaagaagc
     2761 tatcatgaga attgagccta atcaattaga ccaattatta catccaaaat ttgaagataa
     2821 ggcattaaaa gaagccaagg ttatagcaaa aggtttacca gcatcaccag gtgcagcaag
     2881 tggtaaagtt tatttcaatg ctgatgatgt agtaaaagca aatgaaaaag gagaaaaagt
     2941 agttttagtt agattagaga catctccaga agatatagaa ggaatggtta aagcagaagg
     3001 aatccttaca gctagaggtg gaatgacatc acatgcagca gttgttgcta gaggtatggg
     3061 taaatgttgt gttgctggat gtggagaaat aaaagttgat gagtttaata aagaaataag
     3121 agcacttgat gatgtagtta taaaagaagg tgaatacatt tctatagatg gttctacagg
     3181 aaatgtgtac ctaggagatg ttaagaaaac agaagtatct ttaactggaa actttgaaaa
     3241 attaatgaac tgggtagaca aacacaaatg tatgatggtt agaactaatg cagataatcc
     3301 tagagatgct cgtgcagcta tagagtttgg tgcagaaggt ataggtctat gtagaactga
     3361 gcatatgttc tttgatgaag ctagattgcc agcagttaga gaaatgatat tatctaatac
     3421 agtagaacaa agagaaaaag ctcttgaaaa aatattacca atgcaaagag aagattttgt
     3481 agagttattt aaagttatgg atggaaaacc agttaatata agattactag acccaccact
     3541 acatgaattt ttgcctcatg atgatgagac tatagaagaa ctttcaaaat ctatgggaat
     3601 aaaagtaagt gatattaaga agagaatagt tgatttagat gagtttaacc caatgcttgg
     3661 acacagaggt tgtagacttg caataactta tcctgaaata tgtgtaatgc aatctaaagc
     3721 tataatacaa ggtgctatag aagcaatcaa agcaggtgtt aaagtttctc cagagataat
     3781 ggttccatta gttggagaag taaatgagtt aaaaataata agaaaaatga tagtagaaac
     3841 tgttgacgct ataataaaag aagaaggtat agaagttcct tatactgtag gtactatgat
     3901 agaaatacct agagcttgtt taactgctga tgaaatagca caagaagcag acttcttcag
     3961 ttttggtact aatgacttaa ctcaaatggc atttggatat tcaagagatg atgctggtaa
     4021 attcttaggt caatatgtag atgaagaaat attagaaaaa gacccattcc aagtactaga
     4081 ccaaaatggt gttggtaagt tagttaagat gggagctaaa ttaggtagag aagttaaacc
     4141 agaattaaaa ctaggaatat gtggagagca tggaggagaa ccatcttcag tagagttctg
     4201 ttatagtgta ggacttaact atgtatcttg ttcaccattt agagtgccaa ttgctagact
     4261 tgcagcagct caagcatcta taaaaaatcc tagataataa tagactgcaa aatatgtaac
     4321 taaataaaaa taaaatcccc tattgttcaa tgtatcaaat taagtatttg taaattgaat
     4381 ataggggatt tttattataa aaatttaact tatacagttg taagtgaaac gtatttaatt
     4441 atattgcata tattattgta gagagaaatt tcaactatca attaaattga gtttactgga
     4501 ggggataaag ttgacaaagt gtcctaaatg tggaaaggaa gtatcatcac gtccaggtag
     4561 tatatgccca cgatgtggat ttaaagtagc agatgagagt ctaaaaataa gatgtccaga
     4621 acctagttgt agggctttgg tatctaggaa actggattat tgtcctaaat gtggatgcaa
     4681 acttagagga tataatattt ctgaatttgt taacatggta agatgtaaaa tagatgagac
     4741 atttgataaa aaataattaa atttagagtt aagcttttga gggtgtctca aaatgaactt
     4801 tttagttcag aaggcactct ttttgtatgg aatcaaatat atttttataa tttaaacaat
     4861 aaaaaagagg cttatctcta ttttagtata atccccttat agtagacatt taaataaaag
     4921 gctattataa taagtctaaa aagcgtatac tctaaggggg tttttctatg tctaaaaaac
     4981 ataaaaaata tagcaaagaa cttaaactaa aagctgtaaa tttatatata aaagaaggat
     5041 actcatcata caaaattgct gagatgctga atataagaag c
//
LOCUS       DAMJWP010000238         5065 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.245, whole genome shotgun sequence.
ACCESSION   DAMJWP010000238 DAMJWP010000000
VERSION     DAMJWP010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5065
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.245"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            14..1897
                     /locus_tag="PC700_003228"
     CDS             14..1897
                     /locus_tag="PC700_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HDF2582498.1"
                     /translation="MFNKNKENITLSNQIKGTIQNSLTKEAILHIPMSNYAYGYDRET
                     LHIRLRTKKNEAKRVILRIGDQYVWDKGGAGGGNLNASGVGWSGGTNIVMSKEVETEL
                     FDYWIAKCKPLNKRSRYGFIIEGQEEKLLFTEKRIIELGNENDEKELCEIGNFFGYPY
                     LNYIDIPKVPNWVKDTVWYQIFPDRFANGDPSINPEGVDKWGAIPTRDNFTGGDLQGV
                     IEHLDYLSDLGINGIYFCPITIGKTNHRYDTVDYMEVDPTLGDKETLKKLIEEAHKRN
                     IKIMLDAVFNHIGYYSKQWQDVVQNKENSRYKNWFYIKDMSKIDTPIEQIDEKNIPYE
                     TFGCEKYMPKLNTENSEVIKYLLDVGKYWIQEFDIDAWRLDVSNEVDHVFWRKFREEV
                     KKVKPDIYILGEIWHGSLPWLMGDQFDSVMNYLMSEAMKKFFCTDEINAEEFKYMIND
                     VMVSYPIQVSEVIFNLLGSHDTTRILTYANGNIDRFKLAYMFMFVQTGCPCIYYGDEI
                     GMEGELSSISEGQRKCMEWNEEKWNKDIFDFMKKIIRLRKENKELRSISNEWVLADKE
                     NGTIILKKESISIIINNSSKNIILTLPDYLANKKVKDLYEEEIVDLKEELMLEKYKFI
                     ILK"
     gene            2121..2801
                     /gene="pgmB"
                     /locus_tag="PC700_003229"
     CDS             2121..2801
                     /gene="pgmB"
                     /locus_tag="PC700_003229"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDF2582499.1"
                     /translation="MIEAFIFDLDGVITDTAYYHYMAWRKLAHKVGIDIDTKFNESLK
                     GISRMESLDRILEFGNKKYSFSEEEKVRMAEEKNNYYVSLIDEITSNDILPGIESLLI
                     DVKSNNIKIGLSSASKNAINVLNHLGISDKFDFIADAGKCKNNKPHPEIFLMSAKGLN
                     VNPQNCIGIEDASAGIDAINSANMFSVGVGNYENLKKANLVVDSTNQLKFEYIQEKYN
                     EYIVRRII"
     gene            2798..>5065
                     /locus_tag="PC700_003230"
     CDS             2798..>5065
                     /locus_tag="PC700_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_097546124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDF2582500.1"
                     /translation="MKCLFSNVDEWKIIQDKIEIKENRLAESIMSIGNGYMGMRGNYE
                     ENYSGDTHKGSYIAGVWFPDKTRVGWWKNGYPEYFGKIPNSVNYIGVRIFIDDVELDL
                     AKCKVEDFYRELDMKNGILNRRFIVNINDKKFEVRVTRFLSISVKELAVIKYSIKSLN
                     SNTKIKFVPYLDSNIKNEDSNYEEKFWNGISSSSLEYKGSILSRTKENAFNTPVFTVG
                     SMMYIKTNGDVEAVSHESDYNYCENSITMDVLKNKVASIEKYVSITSTRDYKESELLR
                     KCEDILNIELSKSYDDILNEHTKLWNKRWESADVKINGDKSSQQGIRYNLFQLFSTYY
                     GEDSRLNIGPKGFTGEKYGGATYWDTEAFCVPVYLGVADKEVTRNLLEYRYNQLEQAK
                     DNAKKLGLKGALYPMVTFDGVECHNEWEITFEEIHRNGSIVYAIYNYTNYTGDYKYIK
                     EKGIDVIIEIARFWASRVHLSTKKDLYMIHGVTGPNEYENNINNNWYTNCIAKWCLDY
                     AVENILRLEKECNECIDRNNVTREEVEFWKIISNKMYLPYDEELQIIIQNDNFLDKEF
                     VEVKDLPKENFPLNQNWSWDKILRSCFIKQADVLQGIYYFGNRFTKEEKKRNFDFYEK
                     YTVHESSLSSSIYSIIASEIDNLEKAYELYSRTARLDLDNYNNDTNDGLHITSMSGSW
                     LSIVHGFAGMRTWNETLSFDPKLPKEWKNYSFNINYRDNIINVDVCSDLIKIENLKGK
                     PIKIIVYGKKYILEKY"
BASE COUNT         2096 a          455 c          956 g         1558 t
ORIGIN      
        1 gggaaatttt aaaatgttta ataaaaataa agaaaatata actttaagta atcaaatcaa
       61 aggaacaatt caaaatagtt taacaaaaga agctatatta catataccaa tgagcaatta
      121 tgcctatggt tatgatagag aaacactaca tataagactt agaactaaaa aaaatgaagc
      181 gaaaagagta attttacgaa taggagacca atatgtgtgg gataaaggag gtgcaggagg
      241 aggaaattta aatgcatctg gagttggttg gtctggtgga acaaatatag taatgagtaa
      301 agaagtagaa actgaattat ttgactattg gatagctaag tgtaagcctt taaataaacg
      361 ctcacgatat ggttttataa tagaaggtca agaagaaaaa cttttattta cagaaaaaag
      421 aataatagag ttgggaaatg aaaatgatga aaaggaatta tgtgaaatag gtaatttttt
      481 tggatatcca tatttaaatt atatagatat acctaaagtg ccaaattggg tgaaagatac
      541 tgtttggtat cagatatttc cagatagatt tgcaaatggt gacccgtcta taaacccaga
      601 aggagtagat aaatggggag ctattccaac cagagataat tttacaggtg gagatttaca
      661 aggtgtgata gaacacttag attatttgag tgatttggga ataaacggaa tttacttttg
      721 ccctattaca attggaaaaa ccaatcatag atatgatacg gtagattata tggaggtaga
      781 ccctacatta ggagataaag agactttaaa aaaactaata gaagaagcgc ataagagaaa
      841 tataaaaata atgttggatg ctgtttttaa tcatatagga tattattcta aacagtggca
      901 agatgtagtt caaaataagg aaaattctcg atataaaaac tggttttata taaaagatat
      961 gagtaaaatt gatactccaa tagaacagat agatgaaaaa aatatacctt atgaaacctt
     1021 tgggtgtgaa aaatatatgc ctaaacttaa tacagaaaat tcagaagtaa taaaatattt
     1081 attggatgta ggcaagtact ggatacagga atttgatata gatgcgtgga gattagatgt
     1141 ttcaaatgaa gtagaccatg ttttttggag aaagtttaga gaagaagtca aaaaagtaaa
     1201 accagacatt tatatattgg gagaaatttg gcatggtagt ttaccatggc taatgggtga
     1261 ccaatttgat tcagttatga attacttgat gtcagaagcc atgaaaaaat tcttttgtac
     1321 agatgagata aacgccgaag aatttaagta tatgataaat gatgtaatgg taagttatcc
     1381 aatacaagtt agtgaagtta tttttaattt acttggaagt cacgatacta caagaatatt
     1441 gacttatgct aatggaaata ttgatagatt taaacttgca tatatgttta tgtttgtaca
     1501 aacaggatgt ccttgtatat actatggtga tgaaatcgga atggaaggag aattaagttc
     1561 aatatctgaa ggtcaaagaa aatgtatgga atggaatgaa gaaaaatgga ataaggatat
     1621 atttgatttt atgaagaaga ttattagact tcgtaaagaa aataaagaac ttaggtctat
     1681 atcaaatgaa tgggtgttag cagataagga aaatggaacg attatattga aaaaagaatc
     1741 tatttctata ataataaata attcatctaa aaatataatt ttgacgttac cagattattt
     1801 agcaaataaa aaagtaaaag atttatatga agaagaaata gttgatttaa aagaagaact
     1861 aatgttagag aaatacaagt tcattatttt aaaataatac aaaaagtatt tattttagat
     1921 ttcatgttaa attattatac agtttaaaca agtctttata aaacataaaa aaatatggtt
     1981 tacaagtgag tgaattgaaa gtagatttta aattaaagac tgagttagag tagataattt
     2041 tataaataat atcttaaata caagatagaa aataaattga ttattagtct aaaaatttct
     2101 tgtagaaaga ggaaataaga atgattgaag catttatatt tgatttagat ggtgtaatta
     2161 cagatactgc atattatcac tatatggctt ggagaaaact agcccataaa gttgggatag
     2221 atatagacac aaaatttaat gaatctttaa aaggcataag tagaatggag tcattggaca
     2281 ggatattaga atttggaaat aagaaatatt cattttcaga agaagaaaaa gtgagaatgg
     2341 ctgaagaaaa aaataattac tatgtatcat tgatagatga gataacttca aatgatattt
     2401 tgccaggcat agaaagcttg ttaattgatg taaaatctaa taatataaag ataggtctat
     2461 cttctgcaag taagaatgca ataaatgtat taaatcattt gggtataagt gacaaatttg
     2521 attttatagc tgatgcagga aaatgtaaaa acaataaacc acatccagag atatttctta
     2581 tgtcagcgaa agggcttaat gtaaatccac aaaattgtat agggatagag gatgcaagtg
     2641 caggtataga cgctataaac tcagctaata tgttttctgt tggtgttgga aattatgaaa
     2701 acttaaaaaa agcaaattta gttgtagatt caacaaatca attaaagttt gaatatatac
     2761 aagaaaagta taatgagtat atagttagga gaattatatg aaatgtttat ttagtaatgt
     2821 agatgagtgg aagattatac aagataagat tgaaatcaaa gaaaatagat tagcagagtc
     2881 cataatgagt ataggcaatg gatatatggg tatgagagga aactatgagg aaaattatag
     2941 tggagataca cacaaggggt cttatatagc tggagtatgg tttccagata aaactcgtgt
     3001 tggttggtgg aaaaatgggt atccagaata ctttggtaaa attcctaatt cagtcaacta
     3061 tataggtgta agaattttca tagatgatgt agaattagat ttagcaaaat gtaaagtaga
     3121 agatttttat agagaattag atatgaaaaa tggtatttta aatcgtaggt ttattgtaaa
     3181 tataaatgat aaaaaatttg aagttagggt tactaggttt ttaagtatat ctgttaagga
     3241 acttgcagta attaaatata gtataaaatc attaaattct aatactaaaa taaaatttgt
     3301 accatattta gattcaaata taaaaaatga agattcaaac tatgaggaaa agttttggaa
     3361 tggaatttct tcaagttctt tagaatataa aggtagtatt ctttctcgaa caaaggagaa
     3421 tgcatttaat acaccagtat tcactgtggg ttcaatgatg tatattaaga caaatggtga
     3481 tgtagaagct gtatctcatg agagtgatta taattactgt gaaaattcga taacaatgga
     3541 tgttttaaaa aataaggttg caagtataga aaaatatgta tctataactt caactagaga
     3601 ttataaggaa agtgagctat taagaaaatg tgaagacatt ttaaatatag aactatctaa
     3661 aagttatgat gatatattaa atgaacatac aaagttatgg aataaaagat gggaaagtgc
     3721 agatgtaaaa ataaatggag ataaaagtag ccaacaaggt ataagatata atttattcca
     3781 actattttca acatattatg gagaagattc gcgattaaat ataggtccaa aaggatttac
     3841 tggtgaaaag tatggaggag ctacttattg ggatacagaa gcattttgtg ttccagtata
     3901 tctaggtgta gcagataagg aggtaactag aaatctttta gaatataggt acaatcagtt
     3961 agaacaggca aaggataatg caaaaaaact agggcttaaa ggtgctttat atccaatggt
     4021 aacatttgat ggagtagaat gtcataatga atgggagata acatttgagg aaatccaccg
     4081 aaatggaagt atagtatatg caatatataa ctatacaaat tatacaggtg actataaata
     4141 tataaaagaa aaaggaatag atgtaataat agaaatagct agattttggg caagtagagt
     4201 tcatttgtct acaaaaaaag atttgtatat gatacatgga gtaacaggtc caaacgagta
     4261 tgaaaacaat atcaataata attggtatac taattgtata gctaaatggt gtttagatta
     4321 tgctgtagaa aatatcttac gattagagaa agagtgtaat gaatgtattg acagaaataa
     4381 tgtgactagg gaagaagttg agttttggaa aataatatca aataaaatgt atctgccgta
     4441 tgatgaggaa ctacaaataa taattcaaaa tgataatttt ttagataaag aatttgtaga
     4501 agtaaaggat ttaccaaaag aaaattttcc tcttaatcaa aattggtctt gggataagat
     4561 tcttaggtcg tgttttataa aacaagcaga tgtattacag ggaatttact attttggaaa
     4621 tagatttact aaagaagaaa aaaagagaaa ttttgacttc tatgaaaaat atactgtcca
     4681 tgaatcttct ttatcttcta gtatttattc tattattgcc tcagaaatag ataacctaga
     4741 aaaggcttat gaattatact caagaacagc taggcttgat ttagataatt acaataatga
     4801 tacaaatgat ggactgcaca taacttcaat gtctggttca tggctttcca ttgtacatgg
     4861 atttgcaggc atgagaacat ggaatgaaac tctatctttt gaccctaagt tgccaaaaga
     4921 gtggaaaaat tattctttca atataaatta tagagataat attattaatg tagatgtatg
     4981 tagtgattta attaaaatag aaaatttaaa aggtaaacca ataaaaatta ttgtatatgg
     5041 gaaaaaatat atattagaaa aatat
//
LOCUS       DAMJWP010000239         5035 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.246, whole genome shotgun sequence.
ACCESSION   DAMJWP010000239 DAMJWP010000000
VERSION     DAMJWP010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5035
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.246"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..1767
                     /locus_tag="PC700_003231"
     CDS             <1..1767
                     /locus_tag="PC700_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2582501.1"
                     /translation="NGKFQFFKGVRNCFNMIYLHDKRYLFISMFMAIFQGIVPSISLL
                     VSQNIINNMQTFSKSVKEILVLLIMYLIINIIPSIFQSFYGYYRIKFNKNFEKHIETM
                     MLEKSLTLSLKDFEDDNTYDMINRAQSQKGFNITILSENLISIIRESVSTLSLIVVLS
                     QFQLWLIIVVIVIPVLKSLYSIKLGKEQYDVSVNRTWKERKAWYIDYLLSKGYAYKEI
                     MVFRIGSKLINHYKKLKEDIISQDMNIAKKNFIATLLCTVLDSIVSAGIIGFIIIQGI
                     LKKILIGDATTYIQAVQSIKDNVESIFSLFSSVIQDLFYTDLLFKFLDYNCKEDSIEH
                     IKIFEKIGDINKIELINISYKYNHSSEYSLKNINLTLNKNDPIAILGKNGSGKTTLLK
                     ILLGFYDDYEGDILINGKNLKDIEKDEYIRKVSCMFQDYIKYEATLKENVAMGDVYKS
                     NLEEKVKSKLKETKLKPSIYEEDGLETILGTWFGKKQLSMGEWQKVAISRTLMKDADM
                     YIFDEPDSSLDSEAGNELLSLYKKTFENKIGIFITHHIQHAKKITNNIVVLENGKLLE
                     SGRHDELMDKKGVYFNLFCNCK"
     gene            1789..3210
                     /gene="ccpM"
                     /locus_tag="PC700_003232"
     CDS             1789..3210
                     /gene="ccpM"
                     /locus_tag="PC700_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-rich peptide radical SAM maturase CcpM"
                     /protein_id="HDF2582502.1"
                     /translation="MNQKPFIHTFKAGKSYFIYDVNTDKILKVNAAVYNYLNKIEHNL
                     EKESDWNIEIEDEINTLINYGFLKNKRVNKTMHPETKYIKYHIENNLSSIILQVTQNC
                     NLRCDYCIYSGSYSNRVHTNKRMNIDVAKRSIDYLIKNSSQLNEVSVGFYGGEPLLEF
                     DLIKQIVEYVKVVGEGKEIYFNLTTNATLFNEEIIEFFVKNNITTMISLDGGKEIHDK
                     SRKFANTGEGSHDVVLKNVRYIKNKYPEYYKKNITFNTVINSENDYISIDDFISKNET
                     LKDSNFLASTLNNNYSDKEIVSSDEYILGKSYDRFLTLLNKLKFIKDESMSPLSRIER
                     ALLDDFRYEKNIEGRLELPDVWHHGGPCIPGAFRIFVTTDGEFFPCERVSESSKMGMI
                     GNLDSGVDIEKAEKLLNIGKLYEEQCSDCWAYSYCDICIAKIDDTKCNENEIKPHDCD
                     RIRHSTENNMKDYCVLKSLGYQY"
     gene            3239..4309
                     /locus_tag="PC700_003233"
     CDS             3239..4309
                     /locus_tag="PC700_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04066 family peptide maturation system
                     protein"
                     /protein_id="HDF2582503.1"
                     /translation="MEKVLIYPFNYNFFTILNHCNKISDIEINSLVSPSGWGLCNKEY
                     NVNNIITKVTNDFQSELKKNSLVWFVDSKENLDLEKFIIPKLKVALKEGKKILYTRDD
                     LNKISRYISHYEFKKINEWNLEKYEIRDIEGISQINTPIIFICGLYGGLDTFDVNIRI
                     GEELRKRGFSILQFGPKKESYLFKMIPIPEFMFDDSISSKTKIINFNHFIKDKEVEAK
                     PDLIIIEVPGELFPFSRKMVGNFGIRAFEISNAVINDCGILCIPSSIYDREYCEELKY
                     FIKGRHGINIDYFSIEKKVIDTYETEVKQKFSYMSLDENFFVEDIEDYEKTFFIHDNK
                     QLLELVDNIVNQLKEYAKVISI"
     gene            4325..4576
                     /locus_tag="PC700_003234"
     CDS             4325..4576
                     /locus_tag="PC700_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide maturation system acyl carrier-related
                     protein"
                     /protein_id="HDF2582504.1"
                     /translation="MNYTEKELLLENIFFDVTGINFNKNNNLKKELFFSPSLHIQPRE
                     LLLVFCEVIENFNIKIPEEKLLNRQFTSFDNVLKIINMV"
     gene            4627..4830
                     /locus_tag="PC700_003235"
     CDS             4627..4830
                     /locus_tag="PC700_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582505.1"
                     /translation="MSFGKRIEMNNIGVETRGVCTIMCPYTCKMMASSSCNCKNNDYN
                     ARGGSYNTYYNKLIHGQRTPDGM"
BASE COUNT         2044 a          448 c          815 g         1728 t
ORIGIN      
        1 aatggtaaat ttcaattttt taaaggtgta agaaattgtt ttaatatgat ttatctacat
       61 gataaaagat atttatttat ctctatgttt atggcaattt ttcaggggat tgttccatct
      121 atttctttgt tagtaagtca aaatatcatt aataatatgc aaacattttc gaaaagtgta
      181 aaagaaatat tagttttgtt aattatgtat ttaatcataa atataattcc ttcaattttt
      241 caaagttttt atggatatta tagaataaag tttaataaaa attttgaaaa acatattgaa
      301 acaatgatgt tagagaaatc tttaactttg agtttaaaag attttgagga tgataatact
      361 tatgacatga taaatagagc tcaatctcaa aaaggattta atattactat actatctgaa
      421 aaccttattt caataataag agaatcagta tctacacttt ctcttattgt tgtattatca
      481 caatttcaac tttggctaat aatagttgtt attgttattc ctgtattgaa atcattatat
      541 tccattaaat taggaaagga acaatatgat gttagtgtaa acagaacttg gaaagaaaga
      601 aaggcctggt acatagatta tttgttgtca aaaggttatg cttacaaaga gattatggta
      661 tttagaattg gttctaaact tattaatcat tataaaaaat taaaagagga tattatatct
      721 caagatatga atatagctaa aaagaatttt attgctacgc tattatgtac agttttagat
      781 tcaattgtat ctgcaggaat tataggattt ataataattc aaggtatttt aaagaagatt
      841 ttaataggag atgcgactac atatatacaa gcagttcaat ctataaagga taatgttgaa
      901 tcaatatttt cgttatttag ttcagtaata caagatcttt tttatacaga tttactgttt
      961 aaatttctag attataactg taaagaagac tcaattgaac atataaaaat atttgaaaaa
     1021 ataggggata taaataaaat agagttaata aatatttcat ataaatacaa tcattcatca
     1081 gagtattctt taaaaaatat taatttaact ttaaataaaa atgatcctat tgctatacta
     1141 ggaaaaaatg gttcaggtaa aacgacactt ttaaaaatat tattaggttt ttatgatgat
     1201 tatgaaggtg acatcttgat aaatggaaaa aatttaaagg atattgaaaa agatgaatac
     1261 attagaaaag taagttgtat gtttcaagat tatattaaat atgaggctac attaaaagaa
     1321 aatgtcgcca tgggagatgt atataagagt aacttggaag aaaaagttaa aagtaaactt
     1381 aaagaaacta aattgaaacc aagtatttat gaggaagatg gattagaaac tattttagga
     1441 acctggtttg gtaagaagca actttctatg ggtgaatggc aaaaagtagc aatatctaga
     1501 actcttatga aagatgctga tatgtacatt tttgatgaac cagattcatc tttagattct
     1561 gaagcgggaa atgaactcct ttctttatat aaaaaaacat ttgaaaataa gattggaata
     1621 tttataacgc accatatcca acatgcaaaa aagataacta acaatattgt agttcttgaa
     1681 aatggaaagt tgttagaaag tggaagacat gatgaactta tggacaaaaa gggtgtttat
     1741 tttaatttat tttgtaactg caaataaata ttaaagggag gttaattaat gaatcaaaaa
     1801 ccatttatac acacatttaa agctggaaaa tcatatttta tttatgatgt taatacggat
     1861 aagattttaa aggtaaatgc tgctgtttat aattatctaa ataaaattga gcataattta
     1921 gaaaaagaaa gtgactggaa catagaaata gaagacgaaa ttaatacctt gattaattat
     1981 ggatttctaa aaaataaaag ggtaaataaa acaatgcacc ctgaaactaa gtatataaaa
     2041 tatcatatag aaaataatct atcctctatt attttacaag taacccaaaa ttgtaattta
     2101 cgttgtgact attgcattta ttctggttca tacagcaaca gagttcatac aaataaaaga
     2161 atgaatatag atgtagcaaa aagaagtatt gactatttga ttaagaattc tagtcaatta
     2221 aatgaagtta gtgttggctt ttatggagga gaacccttac ttgagtttga tttgattaag
     2281 cagatagtag aatatgtaaa agtagttgga gaaggaaagg aaatatactt taatctaact
     2341 acaaatgcaa ctttatttaa tgaggaaata atagaattct ttgtaaaaaa taatataaca
     2401 actatgatta gtttagatgg aggaaaagaa attcatgata aaagtagaaa atttgcaaat
     2461 acaggagaag gatctcatga tgttgtatta aaaaatgtac gttacataaa aaataaatat
     2521 ccagaatatt ataaaaagaa tattacattt aatacagtga taaattcaga aaatgattac
     2581 atttctatag atgattttat atctaaaaat gagactttaa aagattcaaa ctttttagct
     2641 tccactctta ataataacta ttctgataaa gaaatagttt catcagatga atatatatta
     2701 ggtaagagtt atgatagatt cttgacttta ctcaacaaat taaaatttat aaaagatgag
     2761 agtatgtcac ctttatcaag aatagaaaga gccttattag atgattttag atatgagaaa
     2821 aatattgaag gtagattaga gttaccagat gtgtggcatc atggtggacc atgtatacca
     2881 ggggctttta gaatatttgt aactacagat ggagaatttt ttccatgtga gagagtaagt
     2941 gaaagttcta aaatgggtat gattggaaat ttagatagtg gagtagatat agaaaaggct
     3001 gaaaaacttt taaatatagg aaaattatat gaagagcaat gttctgattg ttgggcctat
     3061 agttattgtg atatatgtat agctaagatt gatgatacta aatgtaatga aaatgaaata
     3121 aaaccacatg attgtgacag aattagacat tctacagaaa ataatatgaa agattactgc
     3181 gttttgaaat ctctaggata tcaatattaa attttctaat gaaaggaaag atgggattat
     3241 ggaaaaagta cttatatatc catttaatta taattttttt actattttaa atcattgcaa
     3301 taaaatttca gatatagaaa ttaattcact ggttagtcct tctggctggg ggttatgcaa
     3361 taaggagtat aatgttaata atattattac taaagttaca aatgattttc aaagtgaact
     3421 taaaaagaat tctttggtat ggtttgttga ttcaaaagaa aatctagact tagaaaaatt
     3481 tataattcct aaattaaaag tagctctaaa agaaggtaaa aagatattat atacacgtga
     3541 tgatcttaat aaaatatcta ggtatatatc tcattatgaa ttcaaaaaga ttaatgaatg
     3601 gaatttagaa aaatatgaaa taagagatat agagggtata tcacagataa acacaccaat
     3661 aatatttatt tgtggtcttt atggagggtt agatactttt gatgtaaata taaggatagg
     3721 agaagaacta agaaaaaggg ggttttcaat attacaattt ggtcctaaaa aagagagcta
     3781 tttatttaaa atgattccta taccagagtt tatgtttgat gattcgataa gtagtaagac
     3841 aaagattatt aattttaacc attttataaa agataaagag gtagaggcaa agccagattt
     3901 aataattata gaagttccag gagaattatt ccctttctct agaaaaatgg ttggtaattt
     3961 tggaattaga gcttttgaaa tttctaatgc agtaataaat gattgtggta tattatgtat
     4021 tccatcaagt atatatgata gagaatattg tgaagaactt aaatatttta ttaaaggaag
     4081 acatggtata aatatagatt attttagtat agagaaaaaa gttatagata catatgagac
     4141 agaggtgaag caaaaatttt catatatgtc attagatgaa aacttttttg ttgaagatat
     4201 agaagattat gaaaaaacat tttttataca tgataataaa caattactag aattagtgga
     4261 caatatagtg aatcaattaa aagagtatgc taaagtaata tctatttaga attggaggtt
     4321 aattatgaat tacactgaaa aggaattact actagagaat atattttttg atgtgactgg
     4381 aattaatttt aataaaaata acaatttaaa aaaagaatta tttttttcac cgtctttaca
     4441 tatacagcca agagaattac ttttagtatt ttgcgaggta atagaaaact ttaatataaa
     4501 aattccagag gaaaaacttt tgaataggca atttacgagt tttgataatg ttctaaaaat
     4561 aatcaatatg gtataggtta ataaaggtat aaagtaagtg atcttataga aaaggggagg
     4621 atatgaatga gttttggaaa aagaatagaa atgaataata taggtgttga gacaagaggt
     4681 gtttgtacta ttatgtgtcc ttatacttgt aagatgatgg catcttcatc ttgtaactgc
     4741 aagaataatg attataatgc aagaggagga tcatataata catattataa taaattgata
     4801 catggtcaaa gaactcctga tggaatgtaa atatttcagt aataagtata ctattaaatt
     4861 gatttagcat atttgattaa atttatcttt cttactttat ttccttttat tataaaaata
     4921 tgcagttgat tataattaag agtgtgattt tataataact gcataaatag tatctgttat
     4981 ataaatcacc tcttcaatta ttatagtggt aaatatagtg attaggggtg atttt
//
LOCUS       DAMJWP010000240         5017 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.247, whole genome shotgun sequence.
ACCESSION   DAMJWP010000240 DAMJWP010000000
VERSION     DAMJWP010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5017
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.247"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            40..1509
                     /locus_tag="PC700_003236"
     CDS             40..1509
                     /locus_tag="PC700_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HDF2582506.1"
                     /translation="MSNVNKKLVIAIGAVALVAIIFIGIMCMQFKGEKIAKNTYVNGV
                     DIGKLTKSQAKQELAKKYKLENVEFNYNDKSWKVKSKDLNLSTDLDKTVENAYNLNRK
                     SSFFGNLSKTISTNFGKKSNLVVVINYDKNKLKAEMEKIAKEIDVDVKDATLDISGEK
                     VKVIPDSDGLKMDISKSMENFDNQTKKGNYKNELVVKATPAKVKKEQLTNIDTNLGTY
                     STIFKTSQINRSINIKLATDNISNVLLMPGETFSFNKHTGKRSKENGYKSAPVIMEGE
                     MEEDYGGGVCQVSSTLYNSVLYAGLEIVNVKNHTIPSSYVPKGRDATVADSGIDFLFK
                     NNLKHPVYIKNYVSGNQIVCNIYGSAEDKQNITISTKLDGVSQTTMKRVNDPTMPKGK
                     EKVDKSGRNAYSVSTYRTFNDANGKKIKTEKIANSYYPKKEGIILVGTMEPKPEEKPN
                     TDENKNNQNTNNQNPNNQQKPETPPTDNKPNETQPQPQA"
     gene            1574..2167
                     /locus_tag="PC700_003237"
     CDS             1574..2167
                     /locus_tag="PC700_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HDF2582507.1"
                     /translation="MNLNNITNYVQSICENISNVLDVDVTLVTKDLTRIAGTGIFRGK
                     IGETISEKSVYSLVLKDGKSYVINKEIEENCNKCANRENCKELADICTPVKMGEHILG
                     ILGIAAFNEEQKEKILSKDKELTAFVRSMSDLISFKLDELYKQEVKTIDDLEREAIEN
                     AIKKYGSNTEGMKKVAEALNIGIATLYRKVKKFNIKS"
     gene            2349..3695
                     /locus_tag="PC700_003238"
     CDS             2349..3695
                     /locus_tag="PC700_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF2582508.1"
                     /translation="MPRKILDISLIEDNKSDLTFFNIEEEDGDETLKALKQSYVTTNY
                     KEETLLNLQGALNSKSDTTSKELKLESYKRYNNALDLAYKNYITSAINMVNKALEINP
                     KDIDILNLRGLLKLLKCDFAKSFESFYTALCYENNHISRKYVNLISSEDFKTFLGRYN
                     HSIRFINEELNYESIHILENIIEEEPELIEPYVILSLLYDKLGNVKKREGYLDKLKEL
                     DKDNPVFEKNEEEKEDTSKKEEIRKVKRKKSILPYIIVGCVLIGMGIYLIQSKKRIEN
                     LNNQISSKEEKLSETDKKLGETSKKLEKTNKELNEVKNKEPEREITVADEEDLYYQAL
                     NLKKNKEYEKAIDNFKSVVSSGKTKKYISESIYQLAITNKLLGNKDEAIKYYKKYINT
                     YTKNDQYYDDSYYELGMLYYDNGDLKNAQQTFYSLRSEVPDSMYNNSKIKEILSEK"
     gene            3877..4248
                     /locus_tag="PC700_003239"
     CDS             3877..4248
                     /locus_tag="PC700_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDF2582509.1"
                     /translation="MKVFNQLVIILSIWVIGEYISSFIQGIILIPGSIVGMLLLFLLL
                     QFKVLDLSSIENVSGFFLDNMAIFFIPAGVSLIKSLDLIRENVLVLLLVICLSTLIVM
                     YTTGIIVEKMIKKKEEGQKNV"
     gene            4241..4930
                     /locus_tag="PC700_003240"
     CDS             4241..4930
                     /locus_tag="PC700_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDF2582510.1"
                     /translation="MYNVLQTPVFGIIVTIVFFNLGRYIQRKTSNPICNPLLIAIVGI
                     ILFLSISKIPYENYKIGADSLNFFLGPVTVVLAVPLYKQFELFKKHMFEIIVGIGCGI
                     VISFVSVLIIGKIANSDVSIINSLIPKSITTPMGISLANSLNGIESITVVAIIFTGIF
                     GGMVASTVFKLGNINHPVAKGIALGTSAHALGTTKAFELGEIEGAMSGVSIGVSGTIT
                     VILIPIIMNFI"
BASE COUNT         2156 a          497 c          848 g         1516 t
ORIGIN      
        1 gggatatata aataatttta ttgtaaggag aagtatggga tgagtaatgt gaacaaaaag
       61 ctagttatag ctattggagc tgtagcatta gtagcaataa tttttatagg cataatgtgc
      121 atgcaattta aaggggagaa aatagcaaaa aacacatatg ttaatggagt agatattgga
      181 aaattaacta aatcgcaagc aaaacaggaa ttagctaaaa aatacaaatt agagaatgtt
      241 gagtttaatt acaatgataa aagttggaaa gtaaagagca aagatttaaa tttatctact
      301 gatctggata aaacagtaga aaacgcatat aatttaaata gaaagagtag tttctttggc
      361 aatctatcaa aaactatttc aacaaatttt ggcaaaaaaa gcaacttagt agttgtgata
      421 aattatgata aaaataaatt aaaagcagaa atggaaaaaa tagctaaaga aatagatgta
      481 gatgtaaaag atgctacact tgatataagt ggtgaaaaag tcaaggttat accagattct
      541 gatggtctta aaatggatat ttctaagagt atggagaatt ttgataatca aactaaaaaa
      601 ggtaactata aaaatgagtt agtagtaaaa gctactccag caaaagtaaa aaaagagcaa
      661 cttactaata tagatacaaa tttaggtacg tattcaacaa tatttaaaac ttctcaaata
      721 aatagaagta taaacataaa attagcaaca gataatataa gtaatgtatt acttatgcca
      781 ggagaaactt tctcatttaa taaacataca gggaaaagaa gtaaggaaaa tggatataaa
      841 agtgcacctg taataatgga aggtgagatg gaagaagatt atggtggagg agtttgtcaa
      901 gtttcatcta cgttatacaa ttctgtgtta tacgcaggtt tagaaattgt taatgtaaaa
      961 aatcatacta taccatcaag ttatgttcca aaaggaagag atgcaactgt tgcagatagt
     1021 ggaatagatt tcttatttaa aaataatcta aagcatccag tttacataaa aaattacgtt
     1081 tcaggaaatc aaattgtttg caatatatat ggaagtgcag aagataaaca aaatataacg
     1141 atatcaacaa aattagatgg cgtttcacaa actacaatga aaagagttaa tgaccctaca
     1201 atgcctaaag gtaaagaaaa agtagacaaa agtggaagaa atgcatattc tgtatcaaca
     1261 tatagaacat ttaatgatgc aaatggtaaa aaaattaaaa ctgaaaaaat agctaattca
     1321 tattatccta agaaggaagg tattatatta gttgggacta tggaaccaaa accagaggaa
     1381 aaaccaaata cagacgaaaa taagaataat caaaatacaa acaatcaaaa tccaaataat
     1441 caacaaaaac cagaaacacc accaactgat aataagccta atgagacaca accacaaccg
     1501 caagcttaaa gttctcaaga ataggattta tgtagagtaa taaaaaatat aaatctaaat
     1561 aggagggatt actttgaatt taaataatat aacgaattac gttcaaagta tttgcgagaa
     1621 tatttctaat gtattggatg tagatgttac tttagttaca aaagatttaa ctaggatagc
     1681 aggtacagga atattcagag gtaaaatagg agagacaata tcagagaagt cagtgtactc
     1741 tcttgtttta aaagatggaa aatcctatgt tataaataag gaaatagaag agaactgtaa
     1801 taaatgtgca aatagagaaa attgtaaaga attagctgat atatgtactc ctgttaaaat
     1861 gggagaacat atacttggaa tcttgggtat agctgcgttt aatgaagagc aaaaagaaaa
     1921 gatactttca aaagataagg agttaactgc ttttgtaagg agtatgtctg atttaatatc
     1981 ttttaaacta gatgaacttt ataaacaaga agttaagact atagatgacc ttgagaggga
     2041 agctatagaa aatgccataa agaaatatgg aagtaataca gaaggtatga aaaaagtagc
     2101 tgaggcatta aatataggta tagctacact ctatagaaaa gtaaaaaaat tcaacattaa
     2161 aagttaatag tattgaaaaa gaggtaacct ttttatgaaa agaagaggat tacctttttt
     2221 attatgagaa tggtggaaaa atatgtatag ttttgacttg acatattaaa tatgttatct
     2281 taaatatata ttttagagtg gaggtgagta ttattttagt taaagatata aaacaagaca
     2341 tagaaagcat gcctagaaaa attttagata tatctttgat agaagataat aaaagtgatt
     2401 tgactttttt taatatagaa gaagaagacg gtgatgaaac attaaaagca ttaaaacaga
     2461 gttatgtgac tacaaattat aaagaagaaa ctctattaaa cttgcaagga gctttaaatt
     2521 caaagtcaga cacaacgtct aaggagttaa aattagaatc atataaaaga tataacaatg
     2581 cattggattt agcatataaa aattatataa ctagtgccat taatatggtt aataaagctc
     2641 tggagataaa tcctaaagat attgatatac taaatcttag aggtctttta aagttattaa
     2701 aatgtgattt tgctaaatca tttgagagtt tttatactgc tttatgttat gagaataatc
     2761 atatatctag aaaatatgta aatctaattt catctgaaga ttttaaaact tttttaggaa
     2821 gatacaatca ttctataaga tttataaatg aagaattaaa ttatgagtca atacatattt
     2881 tagagaacat aattgaagaa gagcctgagc taatagagcc atatgtaatc ttatctttgt
     2941 tatatgataa actaggtaat gtaaaaaaga gagaaggata tttagacaaa cttaaagagc
     3001 tagataaaga taatcctgta tttgaaaaaa atgaagaaga aaaagaagat acaagtaaaa
     3061 aggaagaaat aagaaaagta aaaagaaaga aaagtatttt accatatatt atagttggct
     3121 gtgttttaat aggtatgggg atatatttga ttcaaagcaa aaagagaata gaaaacttga
     3181 ataaccaaat aagtagtaaa gaggaaaagt taagtgaaac tgataaaaag ttaggagaaa
     3241 cgagtaaaaa attagaaaaa acgaataagg aattaaatga ggttaaaaat aaagaaccag
     3301 aaagagagat aactgtagct gatgaggaag atttatatta tcaagctcta aatttaaaga
     3361 aaaacaaaga atatgaaaaa gccatagata attttaaaag tgtagtttct tctggtaaaa
     3421 ctaaaaaata tatttctgag tcaatatatc aattagcaat aacaaataaa ttattaggaa
     3481 ataaggatga agctataaaa tattacaaaa aatatataaa tacttatact aaaaatgacc
     3541 agtattatga tgattcatat tacgagttgg gtatgcttta ttatgataat ggcgatttaa
     3601 aaaatgcaca acagactttt tatagtttga gaagtgaagt tccagacagt atgtataaca
     3661 attcaaaaat aaaagaaatc ttaagtgaaa aataggttta tatagttaag ttttttgaaa
     3721 caaccaagta tgtattttat taaattaaaa taaaaataaa tttaaaaaga gattgtgtca
     3781 aattataact ttctatttta aagaaagata tttgcttgtt gataaaacta caaatatatg
     3841 atttgaagca atctctttaa tttttggagg tggaaaatga aagttttcaa tcaattagta
     3901 ataattttat ctatctgggt aataggagag tatataagtt cctttataca aggaattata
     3961 cttattcctg ggagtatagt tggtatgcta ttattatttc tactactaca atttaaagtt
     4021 ttagacttga gtagtataga aaatgtaagt ggatttttcc tcgataatat ggctatattt
     4081 tttattccag caggtgtatc acttataaag tcattagacc taataaggga aaatgtatta
     4141 gtgcttttat tagtcatatg tttaagtaca ctaatagtaa tgtatactac tggaattatt
     4201 gtggagaaaa tgataaaaaa gaaggaagaa ggacaaaaaa atgtataatg tattacagac
     4261 accagtattt ggtataatag taacaatagt ttttttcaat ttaggaagat acattcaacg
     4321 aaaaacaagt aatcctatat gtaacccttt attaattgca atcgtaggaa taatattgtt
     4381 tttaagcata tctaaaatcc cttatgagaa ttacaagata ggagctgata gtctaaattt
     4441 ctttttaggt cctgtcacag tagtacttgc agttccactt tataaacaat ttgaattatt
     4501 taaaaagcat atgtttgaga ttattgtagg aataggttgt ggtatagtga tttcttttgt
     4561 atctgtattg ataattggca aaatagctaa ttcagatgta agtataataa attcactaat
     4621 tccaaaatct ataacaacac caatgggaat atctttagca aattctttaa atggtataga
     4681 atcaataaca gttgtagcca taatttttac aggtattttt ggtggtatgg ttgcttctac
     4741 agtattcaaa ctggggaata taaatcatcc agttgctaaa ggaattgctc ttggaacatc
     4801 tgcacatgct ttaggaacta caaaagcttt tgaacttgga gaaatagaag gagctatgag
     4861 tggtgtttct attggagttt ctggtacaat tacagtaata ctaattccaa ttataatgaa
     4921 ttttatataa aaaataaaat taagacgagt accttgaata cttaaaacaa aataaaataa
     4981 aaaaaattta aaataaaaac tgaacgaaaa aaatgca
//
LOCUS       DAMJWP010000241         4931 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.248, whole genome shotgun sequence.
ACCESSION   DAMJWP010000241 DAMJWP010000000
VERSION     DAMJWP010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4931
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.248"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            98..901
                     /locus_tag="PC700_003241"
     CDS             98..901
                     /locus_tag="PC700_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582511.1"
                     /translation="MEKLRVIEMIKNNPNIIATIDNPTDEMKLLAIKENGLVLEYINN
                     PTREMQELAIDNNIRAIKFIDNPTEDMMIKAVNEGWSILDYIKNPTDKVIEMAINQAG
                     WAIKYAKNPSEELQLLAVRKNYDSIKFIKQPYESVQEEAVRISYDALRYIDSPSYNAE
                     LIAIKNNEAAISFITDLDKNKMLEFLKVNILVIKYILKKVSKDELESVLKEVLSKEDV
                     EEKYIRDFLNCSVIDRNSESIPIDKIMFIYKYGSKTAKKIAVDEKLKIM"
     gene            913..2043
                     /locus_tag="PC700_003242"
     CDS             913..2043
                     /locus_tag="PC700_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2582512.1"
                     /translation="MNFIDTLKSVDLILSTGEVPLIVGESGIGKTALANKLAKENDWS
                     LIVIDGNLLKEGEIGGLPTIESYVGVNSNGYKTEKKTTVYAVHNKLREIDEEISKSKT
                     VLLFIDEINRCEHTVQQELMNLILNREINGYKLHDDVKILAAMNPSSKYGSDFDYQVV
                     DMDAAQENRFVWLNMESDHTQWIKWAIDEGIERKVIEFISTFPEYLHKINEDDVRATP
                     RSYERVSKIYKVYKEKNNSIPRAVFLNVVKGNVGKVIAEEFISFIESNSEPLISYEDV
                     FLRESIDEPIVERVKNESHTRLYLSAMNILKDLELNIKNDKYESNHYINRFIEFLKMY
                     PVDLMIGIMKDIRNSYIEVYKKAIENEGFVKSYFESYSLIRG"
     gene            2047..3456
                     /locus_tag="PC700_003243"
     CDS             2047..3456
                     /locus_tag="PC700_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582513.1"
                     /translation="MENYFDKQAKELYNKASEIINTHSMLKANRANEKFDMDIPQDFK
                     YEFFSLVDKVNLSLMEEEDNFYGYFLFQMSREIRFDISSPTGVNFKGAKYVIYFNPII
                     FLTLDIKQMETTIKHEIHHILSMHLMRAKELKGKYSTLAINMAMDIVVNKFLNNLPPY
                     ATTLEWVNLKYSLKLEPYEPFEYYVEKIQTELDLMEVDEDGEEDDSDKYGDVEIDYSP
                     ERTHDIWDESNEIDKNTLREFTEKFINSSQKGKVPAYLESMISSLKNSKGELPWNLYL
                     NRIMGTVENNKKKTITRRNRRQPNRLDLRGELRSHKAEIAVALDVSGSISDEEFKQAI
                     KEVLSIVKNYNHEITIIECDNEIRRVYKVRTEKDIKDRIRIGGGTRFSPVFEYANNKK
                     INLLVYFTDGKGENKLQVIPRGYKILWVISGRGDKISLKEPYGAVKKLTKVKTNDEIL
                     EINDVRNDGYSMNSQAPIL"
     gene            3667..4176
                     /locus_tag="PC700_003244"
     CDS             3667..4176
                     /locus_tag="PC700_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HDF2582514.1"
                     /translation="MVMFLFKIDDYIMYGMTGVCKVMDITNERFTNGIKKEYYVLSPI
                     YSNNTVIKIPVDNEKVPMRKLLSKINVLSLINDIPNMDTSWIDNEKLRSEQFKKILRG
                     GKCEELIKLVRSIDNNREYVKSIGKKTHQADDNIMKEAERLLSEEFATILDISPNEVS
                     SYISSHIPQ"
BASE COUNT         2057 a          418 c          857 g         1599 t
ORIGIN      
        1 aagtatatta attataataa gaaacaatta tataaatagg aatagattat tataattaat
       61 atagatataa aaataattat agaaagaaag gcacattatg gaaaaattga gagttattga
      121 aatgataaaa aataatccca atattatagc tacaatagat aatccaactg atgaaatgaa
      181 attgttagct attaaagaaa atgggcttgt actagaatat ataaataatc caaccagaga
      241 gatgcaagaa ctagcaatag ataataatat tagagcaatt aaatttatag ataatccaac
      301 agaagatatg atgataaaag cagtaaatga gggatggagt atactggatt atatcaaaaa
      361 cccaactgat aaagttattg aaatggctat aaatcaagca ggatgggcaa ttaaatatgc
      421 taaaaatcca agtgaagaat tacaactgtt agcagtaaga aaaaattatg attcgataaa
      481 gtttataaaa caaccttatg aaagtgtaca agaggaggca gtaagaataa gttatgatgc
      541 cttgagatat atagattcac ctagttataa tgcagaactt atagctataa aaaataatga
      601 agcagctatt agctttataa ctgatttaga taaaaataaa atgttggaat ttttaaaagt
      661 gaatatttta gtgattaagt acattctgaa aaaagtatct aaagatgaat tagagagtgt
      721 attaaaagaa gtattatcca aagaagatgt tgaggaaaag tatattagag attttttgaa
      781 ttgtagtgtt atagatagaa atagcgaaag tataccaata gataaaatta tgtttattta
      841 taaatatgga agtaagacag caaaaaaaat agctgtagat gagaaattaa agattatgta
      901 ggagagagaa aaatgaattt tatagacaca ttaaaaagcg ttgacttaat attatccact
      961 ggagaagtac ctttaatagt aggtgaaagt ggaataggaa aaacagcttt agcaaataag
     1021 cttgccaaag aaaatgattg gagtttaatt gttattgatg gaaacttact taaagagggt
     1081 gaaataggtg gtcttccaac tatagaatct tatgtaggag ttaattctaa tggatataag
     1141 actgaaaaga aaacaacagt gtatgctgtt cataacaaat taagagaaat tgatgaagag
     1201 atatctaaaa gtaagacagt tcttttattt atagatgaga taaatcgttg tgagcataca
     1261 gtacagcaag aacttatgaa tttaatttta aatagagaaa ttaatggata taaactgcat
     1321 gatgatgtaa agattttagc agcaatgaat ccatcaagta aatatggttc agattttgat
     1381 tatcaagtgg tagatatgga tgcagcccaa gaaaataggt ttgtttggtt aaatatggag
     1441 agtgaccata cacaatggat aaaatgggca atagatgagg gtattgaaag aaaagttatc
     1501 gagtttattt ccacttttcc agaatatctg cataaaataa atgaagatga tgtaagagct
     1561 actcctagaa gttatgagag agtatctaaa atttataaag tttataaaga aaaaaataat
     1621 tctattccta gagcagtatt tttaaatgtt gtaaagggta atgtaggtaa agttattgca
     1681 gaagaattta taagttttat tgaatcaaac tctgaaccat taatatccta tgaagatgtt
     1741 tttttaagag aatctattga tgagcccatt gtagaaagag taaaaaatga gagtcataca
     1801 agactttatc tatcagctat gaacattcta aaagacttag aattaaatat taagaatgat
     1861 aaatatgaat caaaccacta tattaataga tttattgaat ttttaaaaat gtatcctgta
     1921 gatttaatga taggtattat gaaggatatt agaaacagtt atattgaagt atataaaaaa
     1981 gccatagaaa atgaaggttt tgtaaaatca tattttgaat cttatagctt aataagggga
     2041 taatgtatgg aaaattattt tgataaacaa gcaaaagagc tttataataa agcaagtgaa
     2101 attataaata ctcattctat gctaaaggca aatcgtgcta atgagaaatt tgatatggat
     2161 ataccacaag attttaagta tgagtttttt agtcttgtag ataaagtgaa tttaagtctt
     2221 atggaagaag aagataattt ttatggatat tttttgtttc agatgtcaag agaaataaga
     2281 tttgatataa gtagtcctac aggcgtgaat tttaagggag ctaaatatgt tatatatttt
     2341 aatccaataa tctttttaac gcttgacata aagcaaatgg agactacaat taaacatgaa
     2401 attcatcata tattatctat gcatttgatg agagcaaagg agttaaaagg aaagtatagt
     2461 acattggcaa ttaatatggc aatggatata gtagtaaata aatttctaaa caatttacca
     2521 ccatatgcaa ctaccttaga gtgggtaaat ttaaaatatt ccttaaaact tgaaccatat
     2581 gaaccttttg aatattatgt agaaaagatt cagactgaat tagatttgat ggaagtggat
     2641 gaagatggtg aggaagatga tagtgataaa tatggagatg tagaaattga ttatagcccc
     2701 gaaagaactc atgatatatg ggatgaatcc aatgagatag ataaaaatac ccttagagaa
     2761 tttactgaaa aatttattaa cagttctcaa aaaggtaaag ttcctgctta tttagaaagt
     2821 atgatatcat cacttaaaaa tagtaagggt gaattgccat ggaatttata tcttaataga
     2881 ataatgggaa cagttgaaaa taataaaaag aagactataa caagaagaaa tagaagacaa
     2941 ccaaatcgtt tagatttgag gggagaactt agaagtcata aggcagaaat agcagttgca
     3001 cttgatgtaa gtggaagtat tagtgatgaa gaatttaaac aagctattaa agaagttctt
     3061 agtatagtga aaaactataa tcatgaaatt acaattatag aatgtgataa tgaaattaga
     3121 cgtgtatata aagtgagaac tgaaaaagat ataaaagata gaattaggat aggtggaggt
     3181 actagatttt ctccagtctt tgagtatgct aataataaaa agattaattt gttagtctac
     3241 tttactgatg gtaaaggtga aaataaacta caagtaatac ctagagggta taaaatttta
     3301 tgggttattt ctggaagagg agataaaatt tcattaaaag aaccttatgg agcagttaaa
     3361 aaacttacta aggttaaaac aaatgatgaa atattagaaa taaatgatgt aagaaatgat
     3421 gggtactcaa tgaatagcca agcaccaatt ttataggtat tcacaggaga taagctgtat
     3481 taatttattt tgatatgctt gttataaaaa tctaaaaaat acattaatca atggttaatg
     3541 tattttttaa ttttaataat ataagtttta cttacattta agtcttaaaa tgtgttaaaa
     3601 tataatatat agcagatagt tttggcaaga tggatttagt tgtcaaaact atttttttta
     3661 ttaaggatgg tgatgttttt gtttaagatt gatgattata taatgtatgg tatgacagga
     3721 gtctgcaaag tcatggatat aacaaatgaa cgatttacaa atggaataaa aaaagagtat
     3781 tatgtattga gtcctattta ttctaataat acagtaataa aaattcctgt agataatgaa
     3841 aaagtaccaa tgagaaagtt actttctaaa attaatgtgc tttcattaat aaatgatatt
     3901 cctaatatgg atacatcatg gatagataat gaaaaattaa gaagtgagca atttaaaaaa
     3961 atactcagag gtggtaaatg tgaagagtta ataaaattag taagaagtat agataataac
     4021 agagagtatg taaaatctat tggtaaaaaa acacatcaag cagatgataa tataatgaaa
     4081 gaagcagaaa ggttattaag tgaagaattt gcaactattt tagatatttc tcctaatgaa
     4141 gttagttcat atatttcaag tcatatacct caataaagtg ttttagtatc ctttatttaa
     4201 tctattttaa gtattattta tgtatgctgg ttttggtaaa ataaaatata taattaaatt
     4261 atataaaaaa ttaagaatga ttgtattgaa gaattacaga atagtgcgga gagcccactc
     4321 ctttaggtgt gagatggata tcacttaaag atttaaattg ttattgtaga tgtttttaat
     4381 ataaagcaag attaataagt gtattgtaag ctttaagtga aattgatact ataatgagtt
     4441 acttagattt taaaatatat aatattataa aaaatccttt taggttttga atgaataaga
     4501 tggatactat gttgaataga atccatctta tttaagtttt atttatgttg tttatttgta
     4561 agcattattt ttgtacatga aaacacagtg taaaaatatg cttagaatta tgaagtttta
     4621 ttaaaaatat attcgacaaa ataagactaa aaatattctt aaacttataa ctaagataaa
     4681 tattgtatat ttatagtgta gcctgaaaaa ttgtggatgt acgaaattta tttagtattt
     4741 tattgaaaca aagtagaaga agtactatag tttatctttg aatagattgt tattttttag
     4801 tgaaaaagtt attaggttaa tacataaata taatagctta taaatagttt aagcatgtta
     4861 aaaaagtagt tattagagat atatagttac attgcttata agatacttaa aatgatagca
     4921 ttcacttata t
//
LOCUS       DAMJWP010000242         4902 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.249, whole genome shotgun sequence.
ACCESSION   DAMJWP010000242 DAMJWP010000000
VERSION     DAMJWP010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4902
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.249"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            305..3403
                     /locus_tag="PC700_003245"
     CDS             305..3403
                     /locus_tag="PC700_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HDF2582515.1"
                     /translation="MDVINLIDELDEIVYVSSLDTYELLYINEIGKKAMGINDISHVK
                     CYKALQGKDSPCEFCTNSILKEDEYYIWENTNTRTNRHFILKDKLIKWEGKHARLEIA
                     LDITEKEDISKSIAEKLEIKELLVGCIKHLITESNFSDAVNLVLTNIGKFHKADRAYV
                     FEVSEDRKKVTNTYEWCNEGVDEQKQALTNLDINELKNWFLLFEQKGFVIIDDIENIK
                     EEQPIEYEILKPQKIESLMTSSLKQDDKVVGFIGVDNPHKAVGDTSLLTSLSYFLLTE
                     MKKRKVENKLNYMSYYDFLTGAYNRNKYIKYIDNFQKGKFKSIGVVFVDINGLKDIND
                     NFGHANGDSAIVSACVIIKKYFHSYNVYRIGGDEFIVLCEDISKQLFEELVSWLSEEF
                     KDEMIYSVAVGSIWSDDNIDIHKLIKVADERMYQDKKRYYNKNKLRNNEMEVISDLAD
                     STNNILFKHKELVDKVYFDDLMIKGYSKAYEKLAYNTYDIILSICIDTNLITPIYQSE
                     SNIFSFPIKENLEKTFSYIKKHLIHPDDIILFDDNHLLKSLDSLNNSKQDDLELNFEY
                     RRLGIDKKYYWALIYMLKISSKNKLGEKQTHILVAIKNIDYLMQLHKNQKDILTGLYN
                     YESFCEQTSLMLKRYPDKSYAIIMMDIDKFKVINDIYGVRGGDKALIYVAELIQKNIG
                     SNGICCRMYADVFCIFYESYSDRDVHKLIYKIKNSLNRKKFEYMLVPCFGVYKILDNN
                     IPITNACEMAGYAHKKVKKQSINNYIFYDDTIRNDALEEKQMEREMEYALENGQFHVY
                     LQPKYNLNANKIVGAEALVRWVHPEKGIIPPIKFISLFEKNGFIIRLDMYVWEQVCSL
                     IRKWIDLGEEPVPISVNVSRLHLHNPHFKEIVLSLINKYSIPKSFLELELTESLFIKN
                     IKLFTKIIADLRMDGIVLNMDDFGSAYSSLNMLKNINIDTLKIDCGFFNEEGITDREK
                     IVLRHTIAMAKDLDMDVVAEGVETKEQAEFLVSLDCVVIQGYYYCKPIPSADFEKLLY
                     QK"
     gene            3526..4812
                     /locus_tag="PC700_003246"
     CDS             3526..4812
                     /locus_tag="PC700_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2582516.1"
                     /translation="MIVGIIGPSDSGFKIKDDLKQIDSDLKTKIYVREKVVDTIEVIS
                     KCEDECDAIIFTGCAVYEFIKSKYEISKPHSFVPRSGTSIMKAFWAIKSANIKLDKFS
                     IDVVDRYVVEDALKEFDIKATSVFCNPFSLEVGESELVEWHIKLFEENKTDIMLTGFG
                     AVYNELKERGYPVFRLQATIQLIKESYDKVKSEYALSKARFSQIAVEILSLIDYKEKI
                     DNYYSDMIKKSDMDKLVVNYVRSIQGSLFLLGRNDYVVFVHKGVADNEYNYDKLFNLK
                     REIKDMGFSLSIGIGTGVTAYQAENNAHKALRHSIDSKEIGIYLVDEDENIRGPLASE
                     NELNYSLMLSDEKVIEISKKTGMSCESVAKIIAISENRKSKIYDSKELAEYLNVSERS
                     ARRILNKIVNAGLGRICAKETSIGGGRPKNITEILF"
BASE COUNT         1976 a          450 c          880 g         1596 t
ORIGIN      
        1 aaatgaatga agtattatct tcatatatga ttggaatact taattgaggt ggagatttgt
       61 catttatatg agaattaatt gtaaacacat aataagatag ttaattataa atatattaaa
      121 ttaggtatta caaaactcta ttgtacgatt gacacaataa taattctatt tactaaaaaa
      181 tagaacttta ttaaggtact attttaaaac ataaagaagt ataatatcat tgccaaatat
      241 atagcaaaat tttaaaagct ttaaaaattc aatctggaaa atgaataaaa ggagatatat
      301 ataaatggat gtaataaatt taatagatga gttggatgaa attgtatatg tttcttcttt
      361 agatacatat gaacttttat atattaatga aattgggaaa aaggcaatgg gaattaatga
      421 tatatcccat gtaaaatgtt ataaagcatt acaggggaaa gattctccct gcgaattttg
      481 tacaaactct attttaaaag aagatgaata ttacatatgg gaaaatacga atactcgaac
      541 aaatagacat tttatattaa aggacaagtt aattaaatgg gagggcaaac atgcacgatt
      601 agaaatagct cttgatatta cagaaaagga agatataagt aagtcaatag cagaaaaatt
      661 agagataaag gagttattag ttggatgtat taaacatctt ataacagaat ctaatttttc
      721 tgatgctgta aatcttgtat taaccaatat tggtaaattt cataaagctg atagagcata
      781 tgtttttgaa gtatcagaag atagaaaaaa agtaactaat acatatgaat ggtgcaatga
      841 aggtgttgat gaacaaaaac aggcactaac aaatcttgat attaatgaat taaagaattg
      901 gtttttatta ttcgaacaaa agggttttgt aataattgat gatatagaaa atataaaaga
      961 agaacagcca atagaatatg aaattttaaa acctcaaaaa attgaaagtt taatgacatc
     1021 ttcactaaaa caagatgata aagtagtagg gtttattgga gttgataatc cacataaggc
     1081 tgttggagat acctctcttt tgacatccct atcttatttt ttattaactg agatgaaaaa
     1141 aagaaaagta gaaaataaat taaattatat gagctactat gacttcttga ctggtgcata
     1201 taatagaaac aaatatatta agtatataga taattttcaa aaaggaaaat ttaaatccat
     1261 aggagttgta tttgtagata taaatgggtt aaaagatatt aatgataact ttggacatgc
     1321 taatggcgac tctgctattg tgtcagcatg tgtaataata aaaaaatact ttcattctta
     1381 taatgtttac cgtattggag gagatgagtt tattgtatta tgtgaagata tatcaaaaca
     1441 actatttgaa gaattagtga gttggttaag tgaagaattt aaggatgaga tgatatatag
     1501 tgtagcagtg ggcagtattt ggagtgacga taatattgat atacataagc taattaaagt
     1561 tgctgatgag agaatgtacc aagataagaa gaggtattat aataagaaca aacttagaaa
     1621 taatgaaatg gaagtaatta gtgacttagc tgattcgacg aataatattt tatttaaaca
     1681 taaagagtta gttgataagg tttattttga tgatttaatg ataaaagggt attctaaggc
     1741 ttatgaaaaa ttagcttata atacatatga tataatttta tctatttgca tcgatactaa
     1801 tttaattact ccaatttatc aatcagagag caatattttt agttttccta taaaggaaaa
     1861 tcttgaaaaa actttttcat acataaaaaa acatttaatt catcctgatg atataatatt
     1921 atttgatgat aaccaccttc taaaatctct tgatagtctt aataacagca agcaagacga
     1981 tttagaacta aattttgagt atagacgttt aggaatagat aaaaagtact attgggcatt
     2041 aatttatatg ttaaagatta gtagtaaaaa taagttaggt gagaaacaaa cacatatttt
     2101 agttgctata aaaaatatag attatcttat gcaactacat aaaaaccaaa aggatatatt
     2161 aacaggacta tacaattatg aaagtttttg tgaacaaaca agtttaatgc ttaaaagata
     2221 tcctgacaag agctatgcaa ttataatgat ggatatagat aagttcaagg taattaatga
     2281 catttatgga gttagaggag gagataaggc tttaatatat gttgcagaat tgattcaaaa
     2341 aaacattggt tctaatggta tatgttgtcg tatgtatgca gatgtatttt gtatttttta
     2401 tgagagttac tctgatagag atgttcataa attaatttat aagattaaaa atagcttaaa
     2461 tagaaaaaaa tttgaatata tgttagtacc atgttttggg gtttataaaa ttttggataa
     2521 taatattcca attacaaatg cgtgtgaaat ggcaggatat gctcacaaaa aagtaaagaa
     2581 acaaagtatc aataactata tattttatga tgatactata agaaatgatg cacttgaaga
     2641 aaaacagatg gaaagggaaa tggaatacgc acttgaaaat gggcaattcc atgtatatct
     2701 acaacctaag tataatctta atgcaaataa aattgttggt gcggaagcat tggttagatg
     2761 ggttcatcca gaaaaaggta taatacctcc aattaaattt atttcactat ttgagaagaa
     2821 tgggtttatt atcagattag acatgtatgt gtgggaacaa gtatgttctt tgattagaaa
     2881 atggatagat ttaggtgaag agccagttcc tatctctgta aatgtatcaa gattacattt
     2941 gcataatcca cattttaaag aaattgtttt aagtttaata aataaatata gtataccaaa
     3001 atctttttta gaattggagc ttacagaaag tttatttatt aaaaatataa aattatttac
     3061 taagataata gccgacctta gaatggatgg tattgtttta aatatggatg actttggttc
     3121 agcatattct tcattaaata tgttaaaaaa tattaatatt gatacattga agatagattg
     3181 tggcttcttt aatgaggaag gaatcacaga cagagaaaaa atagttttaa ggcacactat
     3241 agccatggca aaagatttag atatggatgt agtggcagaa ggtgtagaga caaaagaaca
     3301 agctgaattt ttagtgagtt tagattgtgt ggtaattcaa ggttattatt attgtaagcc
     3361 tattccaagt gctgattttg agaagttact ttatcaaaaa taagctaatt atcccactct
     3421 aaatgtaggt aggatggagt agttcaacat aattaaatag aagtagaaaa tagtgaggat
     3481 ataattttta aaaagtataa tatttatgga ttaggagcga agtttatgat agtgggtatt
     3541 ataggtcctt cagactctgg gtttaagata aaggatgatt taaaacagat agacagtgat
     3601 ttaaagacta aaatatatgt aagagaaaaa gtagtagata caatagaagt tatttctaaa
     3661 tgtgaagatg aatgtgatgc tattatattt acaggatgtg cagtttatga atttataaaa
     3721 tctaagtatg agataagtaa gccacattca tttgtaccga gaagtgggac gagtataatg
     3781 aaggcatttt gggcaataaa aagtgcaaat ataaaattag ataaattcag tatagatgta
     3841 gttgatagat atgttgtgga agatgcactt aaagaatttg atattaaagc aacgagtgta
     3901 ttttgtaatc cattttcttt ggaagtagga gagtctgaac ttgtagaatg gcatataaaa
     3961 ttatttgaag aaaataagac ggatataatg ttaactggtt ttggtgcagt atataatgaa
     4021 ttaaaagaaa gaggctatcc tgtatttcgt ttacaagcta ctatacaatt aatcaaagaa
     4081 agttatgata aggtaaagag tgaatatgct ctaagcaagg caagattttc tcaaattgct
     4141 gtcgaaatat tgagtctaat agattataaa gagaagatag ataactatta ttctgacatg
     4201 ataaaaaaat cagatatgga taagcttgta gtaaactatg taagaagcat tcaaggttcc
     4261 ttatttttac ttggaagaaa tgattatgtt gtatttgttc ataaaggggt agctgataat
     4321 gagtataatt atgataagct attcaatcta aagagagaga taaaagacat gggcttttct
     4381 cttagtatag gaattggtac tggagtaact gcgtatcagg ctgaaaataa tgcacataaa
     4441 gcattaagac actccataga ttcaaaagaa attggtatat atttggtaga tgaagatgaa
     4501 aatataagag gacctttagc ttccgaaaat gaattaaatt attctttgat gctatctgat
     4561 gaaaaggtaa tagaaatatc taaaaagact ggaatgagtt gtgagtcagt tgcaaaaatt
     4621 attgctataa gtgagaatag aaaaagtaag atttatgatt caaaagagtt agcagagtat
     4681 ctaaatgtaa gtgaaagaag tgcaagacgt atattaaata aaatagtaaa tgcaggttta
     4741 ggtcgaatat gtgcaaaaga gactagtatt ggtggaggaa gaccaaaaaa tattactgaa
     4801 atactgtttt aagatatttt ataaaactgt ataaatcatt atattttata aaattctaca
     4861 acaaattact atataaagta ttaaagctta tcatgaaaaa aa
//
LOCUS       DAMJWP010000243         4834 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.250, whole genome shotgun sequence.
ACCESSION   DAMJWP010000243 DAMJWP010000000
VERSION     DAMJWP010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4834
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.250"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            141..802
                     /locus_tag="PC700_003247"
                     /pseudo
     CDS             141..802
                     /locus_tag="PC700_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
     gene            complement(1021..2058)
                     /locus_tag="PC700_003248"
     CDS             complement(1021..2058)
                     /locus_tag="PC700_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HDF2582517.1"
                     /translation="MNKKLIKAKIFIWSILAISLSIVFISLLIGTIISHSFNFPFNTL
                     NPKYNYDNYIVAKNESFSDKINDINVDWISGEVRIKRSDSNKIKLIQKIPSNFSKKKL
                     AKIMVDGNSLSIYDNSTKKFFIGINLPKPTILELYLPNKLYDKITLYTTSADIYSTYI
                     RSNTVEANTISGAIGLSGNINKINLDTTSGHIDITKLNSKNATLKSTSGEIQLSGKID
                     VLNLETTNGNINLKDIYNKNLTCSSVSGKMNLSGVFSDIKAESTSSNIKINSSKMLSS
                     LYCETTSANVDLSIPDNPGFTLNFDKVSGVLDSDFELNRHSDLYTYKNGITNLYLSTT
                     NGNLTIIKNNH"
     gene            complement(2051..2737)
                     /locus_tag="PC700_003249"
     CDS             complement(2051..2737)
                     /locus_tag="PC700_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582518.1"
                     /translation="MLDKIRTYIDSSFTGVNETKKVKELKDELFENLKEKYNDQIQDG
                     KTEQEAYNSVISGIGDLSELIESVKEPYSLPSELIEEKKKRALRASIAVALFIISPFL
                     FVVSVVSFGIEPTIAILLMLILIALGVGLLVYNQMTRTNYALYNDNLSNKFKEFEDFE
                     ENYNHINPVYKSFMNAYWIIVVSIYLITSILFNIWSFSWILFIIGAAGVNIVKGIIQL
                     KGYKEEDKYE"
     gene            complement(2749..3069)
                     /locus_tag="PC700_003250"
     CDS             complement(2749..3069)
                     /locus_tag="PC700_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2582519.1"
                     /translation="MIIASDMIRGHTETIILKHLLEKDSYGYEINKSILEKTKGLYEL
                     KEATLYTAFRRLEQSGAITSYWGDEVTGARRKYYSITNIGKEKYIKSREEWNNTKALI
                     DILI"
     gene            3430..4770
                     /locus_tag="PC700_003251"
     CDS             3430..4770
                     /locus_tag="PC700_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2582520.1"
                     /translation="MIKSSLFWNIFIAISFMIEWFLCKKMLDYTSEKRISNKKINFCM
                     VGIIVFADIIYFFDVPPNIRVIISMIITIAFYKISYDVGFIKSVLISLIYWMLLIATD
                     ALSISLTVWLNSINDMSSMGTGDIYRIESIILGKILLIVSLFLYRALKIKLNIEINKR
                     TLFYIVIPIVANIASFFVIFEYIFNPGSGSLIHNFQFVMISVVLLISSITLVLIAKKL
                     QQYNTLVVENNMIKNTLKYENEYYEDIEKKYLKTRLLSHDMKNHILCINAMIKKGIDV
                     TSYLNNLQVEIQSNDLLFNTGNFILDAILSEKKEVCDKNDIELKIGINFSCCEAVELV
                     DICHIFSNILDNAIEACNKIDSGNRVICVKGDVMQNFYLIRVENTKMNKIITKNGDIL
                     TDKKGSLHGLGIKSVKNSVNKYGGNVVIEYDDKKFVVKIAIPLRNVKKAVNHVI"
BASE COUNT         1785 a          532 c          720 g         1797 t
ORIGIN      
        1 taataacaat tcaaaataaa taacttatgt aagaaccgta ggaactacgg ggatagcctg
       61 tggagaatta gtaagacata tgttagtatg caaaattcta tgaagcagga accctgtgac
      121 tttagtcatg ggaggttcag attttggctg aagatgaaga aaataatcat ataaatattg
      181 agatgcaaag agcttttgag aacgattatt tggaaagagc tgaatattat cttagtcgtg
      241 tacatggtag aaagttagaa gaaggtaaag agtataaaga aattggaaaa actattggaa
      301 tacatatact aaatcatgta aaatataatc acatagaaga ttatgtaaat tgcctgagat
      361 taacaatgga cgagcatcca gatatttatt ctagtaaaac tgcattatat tttatagagc
      421 ttccaaaaat tcataaatct gactatatag taaatagaat tatgctatgg ggaaaactta
      481 ttgataatcc ttctagctca gatgtaagag ttatagctaa gaatgattct attattaaaa
      541 aagcacttga tagattaaaa gaattaggta gcaatgaaga atatttatta aatctgaaaa
      601 gaggtgctta tatcatgaat aaaagtagaa attttaaaga agagattgaa agagagacag
      661 caattagagt tgcaaaagaa aaagattata aaataattat aatgttgaaa aaaatggttt
      721 taaattagat gacatattga ataaatttaa tgattttgac ttaagtgaag atgagataaa
      781 agaagtatat gaaaataatt gatgataaat aaaaagtgaa cttatataag tttgctgaat
      841 ctgagcctgg aaatatttat gtgtattttt taatgaataa aaatttattt taaaaacaat
      901 aattagaggt acagttaatt atatttttat gatactgtac ttctagtttt ttctattgta
      961 gatttattat ttactaataa atctacaata gaagtgcttt tgttgtaaga atcattttat
     1021 ctaatgattg ttttttataa tagttaaatt tccatttgta gtgcttaaat ataaatttgt
     1081 aataccattt ttataagtgt ataaatcact atgacgattc aactcaaagt cactgtccaa
     1141 aacaccagaa accttatcaa aatttaaagt gaaacctgga ttatcaggaa tagacaaatc
     1201 gacattggca gaagtagttt cacagtacag agaagacaac attttgctag aattgatttt
     1261 tatattactg ctagttgact cagctttaat gtcagaaaaa actccagata agttcatttt
     1321 tccagaaact gaactacaag taagattttt attgtatata tcttttaaat ttatattgcc
     1381 attagttgtt tctaagttta aaacatcaat tttcccagaa agctgtattt caccagatgt
     1441 tgatttgagt gtagcatttt tactatttag ttttgttata tcaatgtgac cactagttgt
     1501 gtctaaattt attttattaa tattaccaga gagacctatt gcacctgaaa ttgtgttagc
     1561 ttcaactgta ttacttctta tgtaagtgct atatatatct gctgaagtag tatataaggt
     1621 tattttatca taaagcttat tgggcaaata tagttcaagt attgttggtt ttggaagatt
     1681 aattccaatg aagaattttt tagttgaatt atcatatata gaaagagaat ttccgtcaac
     1741 cattattttt gctagttttt tctttgagaa attactaggg attttttgaa ttaatttgat
     1801 tttattagaa tcacttctct taattctaac ttcacctgaa atccaatcaa cattaatgtc
     1861 atttatttta tctgaaaaac tttcattttt agccactata tagttatcat aattgtattt
     1921 aggattaaga gtattaaaag ggaaattaaa agaatgtgaa attatagttc ctataaggag
     1981 tgagataaat acaatagaaa gtgatattgc caggatactc cagatgaaaa tctttgcctt
     2041 tataagcttt ttattcatat ttatcctcct ccttgtagcc ttttagttgt ataattcctt
     2101 tgacaatgtt gacaccagca gcacctatta taaatagtat ccacgaaaag gaccaaatat
     2161 taaatagtat gcttgtgatt agatatatag aaactactat aatccaatat gcattcataa
     2221 atgatttata aactggattt atatgattat aattttcctc aaaatcctca aattctttga
     2281 atttatttga taaattatca ttgtatagag catagtttgt ccttgtcatt tgattataga
     2341 ctaataaacc tactccaagt gctattaata tgagcattaa aagaatagct atagttggtt
     2401 ctatgccaaa acttacaact gagactacaa ataggaaagg gcttattata aaaagagcaa
     2461 ctgctattga tgctcttaag gctcttttct ttttttcttc aattaattca gaaggtagag
     2521 aatatggttc cttaacactt tcaattaact cagataaatc accaattcct gagattactg
     2581 agttatatgc ttcttgttct gttttaccat cttgtatttg gtcgttatac ttttctttta
     2641 gattttcaaa taattcatct ttaagttctt tgactttttt tgtctcatta actcctgtaa
     2701 agctactgtc aatataagta cgtattttgt ctaacatatt ttcacctcct aaattaatat
     2761 atctatcaat gcttttgtat tattccattc ttcacggctt tttatatatt tctctttacc
     2821 tatgtttgtt attgaataat actttcttct agctccagta acttcatcac cccaataaga
     2881 ggttattgca ccagattgtt caagtctacg aaatgctgta tacaaagtag cttctttcaa
     2941 ctcatacaat cctttggttt tttcaagtat agatttatta atctcataac cataactgtc
     3001 tttttcaagt aaatgtttta agataattgt ttctgtatga cctcttatca tgtcagatgc
     3061 gattatcatg aaaatcacct ccaattaaat atataatact ttgatatact tcgcctgtca
     3121 aggtatatca tgagaaaaga cattttattg taatatttat aaggttttat atagaaataa
     3181 tgtaaaaact ttataaataa tatattttat acctagttat agataacttt atatagttat
     3241 tattaaaaaa agcaataaaa agtttaattc aatttagcaa atttagtgat ataattaaaa
     3301 aagaaaagaa atatggactt atttggaata attttgacaa tctatttact acacgtgtag
     3361 agtcgtatat aattaaatta tacataaaat ataaaaaaat aatttagtaa tacgttagga
     3421 gaaagtatta tgataaagtc aagcttattt tggaatattt tcattgcgat atcctttatg
     3481 atagaatggt ttctttgtaa gaaaatgcta gattatacaa gtgagaaaag gatttctaac
     3541 aaaaaaataa acttttgtat ggtaggtatt attgtttttg ctgacataat atattttttt
     3601 gatgtacctc caaatataag agtaattata tctatgataa ttactattgc attttataaa
     3661 atctcatatg atgtgggatt tataaagtct gtattaatat ccttaattta ttggatgtta
     3721 ctaatcgcca cagatgcttt gagtatatct ttaacagttt ggcttaattc tataaatgac
     3781 atgagtagta tggggacggg agatatatac agaattgagt ctataatatt gggaaagata
     3841 cttttgattg tgtctttgtt tttatataga gccttaaaga taaaacttaa tatagaaata
     3901 aataaaagga cattatttta tatagttatt cctatagtcg ctaatatagc atcttttttt
     3961 gtaatttttg aatacatatt taatccaggg tctggtagct taatacataa tttccaattc
     4021 gttatgattt ctgttgtatt gcttatatca agtatcactt tagttttaat tgctaaaaaa
     4081 ttacagcaat ataatacttt agttgtagaa aacaatatga ttaaaaatac tttgaagtat
     4141 gaaaatgagt attatgaaga tatagaaaag aaatatttaa aaactagatt actaagtcat
     4201 gatatgaaaa atcacatact gtgtataaat gctatgatta aaaaaggtat tgatgttact
     4261 tcttatttaa ataatttgca ggtagaaatt cagagtaatg acttgttatt taatactggt
     4321 aattttatat tagatgctat attaagtgaa aagaaagaag tttgtgataa gaatgatata
     4381 gaactaaaaa taggaataaa ttttagttgt tgtgaagctg ttgaattagt agatatatgt
     4441 catatatttt caaatatttt agacaatgca attgaggcat gtaataaaat tgatagtgga
     4501 aatagagtta tttgtgttaa aggtgatgtt atgcaaaatt tttatttaat aagagttgaa
     4561 aatactaaga tgaataagat tattactaaa aatggagata ttttgacaga caaaaaaggt
     4621 tctcttcatg gacttggaat aaaaagtgta aaaaactctg taaataagta tggtggtaat
     4681 gttgtaattg aatatgatga taaaaaattt gtagttaaaa ttgctattcc acttagaaat
     4741 gtgaagaaag cagttaatca tgtgatttaa atgtatataa atttgatgtg tagaaattta
     4801 atatataaga attaatgtat aaaagtgatg tata
//
LOCUS       DAMJWP010000244         4826 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.251, whole genome shotgun sequence.
ACCESSION   DAMJWP010000244 DAMJWP010000000
VERSION     DAMJWP010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4826
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.251"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            2..1249
                     /locus_tag="PC700_003252"
     CDS             2..1249
                     /locus_tag="PC700_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDF2582521.1"
                     /translation="MINLGNNINLTLIKTEKFKSNLISLYVQRLLDEKETTKNALIPS
                     IIASGSAKYPSARAISNKLDDLYGSSMGADAVKRGERQVLSFKVINISEKYLDESIFE
                     EVVEFFNEVINNTLVVDGGFKEEYLNIEKENLREKIQSIINDKKEYAQDKCIEAMCKG
                     ERYSVSEFGYEDEIDSITSRELYEHYKNILKTSPIDIVVEGNFDEDKVVDIISKNLKF
                     EREEIINIPRADFIKNVDEVKVIDEQMEITQGKLVMGYRANVDYADIDKYYALVVGSN
                     VLGGGPHSKLFVNVREKESLCYYIFSSIEKYKSIMFISSGIETKDYDKAVELIKKQVE
                     SVKAGDISDEELENSKLALVNSMKSITDNIGGMSDFVFSQSMAKTNSEVQDIISSIEK
                     VTKKDIVEAIKNIELDTVYFLRN"
     gene            1264..2550
                     /locus_tag="PC700_003253"
     CDS             1264..2550
                     /locus_tag="PC700_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDF2582522.1"
                     /translation="MEKIVNDILKEEVYYEKLQNGLDVYFMPKRGFMKKYAILATNYG
                     SNDLEFVPIGEDKKIRVNEGIAHFLEHKMFEQPDGGDAFDKFSKLGVNANAFTNFTMT
                     AYLFSATENFYESLEHLIDYVQTPYFTDENVEKEKGIIAQEIKMYNDDPDWNVYFNCL
                     KAMYVNYPARIDIAGTVDSIYKITKEELYKCYNTFYNPGNMALFVVGDLDVEKVIDVT
                     KKSNNYKVDKLSKSIERFYPEEPESVKEKEVIEKFPISMPMFNIGFKDSNVGLKGKEL
                     LRKEIVTDILVGMLFKKGSKLYEDLYMQGLINENFGAGFSSQVDYAFSIIAGDSKEPK
                     KVKEIILDYIEKSKKEGLSKEEFERTKKKKIGSFIKCFDSINFIGNSFISYVFKDINL
                     LDYLDVIKDITFEEVEERLKEHFKEEYCVISIVEPK"
     gene            2947..3693
                     /locus_tag="PC700_003254"
     CDS             2947..3693
                     /locus_tag="PC700_003254"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDF2582523.1"
                     /translation="MDRVAFTLFGIDIMWYGILMACGMILGTLIAIKEAKRVGIKDDD
                     VLNIAIIAIPVGLICARIYYVVFNWSYYAQNMSQIFNFRGGGLAIHGGLIGGILAGYI
                     YTKIKNINFLKMADTVILGMPLAQAIGRWGNFINGEAHGGATNLPWGIMVDGVKVHPT
                     FLYESIWDFGIFIVLLLFRKNKKYEGQVIVTYITLYSIGRFFIEGLRTDSLMLGPLRM
                     AQVISLIGVIGGIIAHVYLSKKNKHNISEE"
     gene            3723..4658
                     /gene="rsmH"
                     /locus_tag="PC700_003255"
     CDS             3723..4658
                     /gene="rsmH"
                     /locus_tag="PC700_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDF2582524.1"
                     /translation="MEFHHVSVLLNECIENLNIKPDGVYVDCTMGGAGHSKEIVKKLS
                     DKGLFIGFDQDKNAISTAKERLSEYESRVKFVHSNFENIKEELEKIGVYKIDGVLADL
                     GVSSHQLDEADRGFSYMQDAPLDMRMDVRCEFSAYDVVNTYTEDELTKIIKDYGEDNW
                     AKRIAKFIVEERANKPIETTGKLVDIIKKAIPKKARIDGPHPAKRTFQAIRIEVNNEL
                     GVITKMINDASSIMNKGGRICIITFHSLEDRIVKNAFKHLASDCICPQHLPICQCDKE
                     SEVKIITRKPILPSEEEIEVNPRSRSAKLRVAEKI"
BASE COUNT         1956 a          417 c          900 g         1553 t
ORIGIN      
        1 aataataaat ttaggtaata atattaatct tactttgata aaaacagaaa agtttaaatc
       61 gaatcttata agtttatatg ttcagagact attggatgaa aaagaaacta caaaaaatgc
      121 tctaatacca tcaataatag caagtggaag cgctaaatat cctagtgcta gagcaatatc
      181 caataaatta gatgatttat atggttcatc tatgggagct gatgctgtaa aacgtggaga
      241 acgtcaagta ttgagtttta aagtaattaa cataagtgaa aagtatttag atgagagtat
      301 atttgaagaa gtagtagaat tttttaatga agtaataaat aatacgcttg ttgtagatgg
      361 tgggtttaaa gaagaatatt taaatattga aaaagagaat ttaagagaaa agatacaatc
      421 aataataaat gataaaaagg aatatgcgca agataaatgt attgaggcta tgtgtaaagg
      481 agagagatat agtgtaagtg aatttggtta tgaagatgaa atagatagta taacttcaag
      541 agagttatat gaacattata aaaacatact taaaacatca cctatagata tagttgtaga
      601 aggtaatttt gatgaagata aagttgtaga tataataagt aaaaatctaa aatttgaaag
      661 agaagaaatt ataaatatac caagagcaga ttttataaaa aatgtggatg aggtaaaagt
      721 tatagatgaa caaatggaaa taactcaagg taagttagtt atgggttata gagcaaatgt
      781 ggattatgca gacatagata aatattatgc tttagtagtt gggagcaatg tcttaggtgg
      841 tggtccacac tctaaattat ttgttaatgt aagagaaaaa gaaagtttat gttactatat
      901 attctcttca atagagaagt ataaaagtat catgtttata tcatctggta tagaaactaa
      961 agattatgat aaagctgtag aattgataaa aaaacaagta gaaagtgtaa aagcaggtga
     1021 tatatctgat gaagaattag aaaacagtaa acttgcttta gttaattcta tgaaatctat
     1081 aactgataat ataggtggta tgtctgattt tgttttctct cagtctatgg caaagacaaa
     1141 ttctgaagta caagacataa tatctagtat agaaaaggtt acaaaaaaag atatagttga
     1201 agctataaaa aatatagagc ttgatactgt atacttcttg agaaattaat ggaggagatg
     1261 tctatggaaa aaatagttaa tgatatattg aaagaagaag tttattatga gaaattgcaa
     1321 aatggattag atgtatattt tatgcctaaa aggggtttta tgaaaaagta tgctattttg
     1381 gctactaatt atggttcaaa tgacttagaa tttgtaccaa ttggagaaga taagaaaatt
     1441 agagtgaatg aaggtattgc acattttcta gagcataaga tgtttgagca acctgatgga
     1501 ggagatgcat ttgataaatt ttcaaaatta ggagtaaatg caaatgcatt tactaatttt
     1561 acaatgacag cctacttatt ttctgctact gaaaattttt atgagagttt agaacatcta
     1621 attgactatg ttcaaacacc ttattttaca gatgaaaatg ttgaaaagga aaaaggtata
     1681 atagcacaag agataaaaat gtacaatgat gaccctgatt ggaatgtata ctttaattgc
     1741 ttgaaggcaa tgtatgtaaa ttatcctgct agaattgata tagcaggtac tgttgatagt
     1801 atatataaga taactaagga agagctatat aaatgttata atactttcta taatccagga
     1861 aatatggctt tatttgttgt tggtgactta gatgtagaaa aagttataga tgtaactaaa
     1921 aagtcaaaca attacaaagt agacaaactc tctaagagta tagaaagatt ttatccagaa
     1981 gaaccagaaa gtgtaaaaga aaaagaggtt atagaaaaat tccctatatc tatgcctatg
     2041 ttcaacatag gttttaaaga tagtaatgtt ggtttaaaag ggaaagaact tttacgtaaa
     2101 gagattgtaa ctgatatact tgtagggatg ttatttaaaa aaggaagtaa gctctatgaa
     2161 gatttgtata tgcaaggttt aataaatgaa aattttggag ctggattttc gtctcaagtg
     2221 gattatgctt ttagcataat agctggagat tctaaagagc ctaaaaaagt aaaagaaata
     2281 attcttgatt atatagaaaa atcaaaaaaa gaaggattat caaaagaaga gtttgagaga
     2341 actaaaaaga aaaaaatagg gtcatttatt aagtgctttg attctataaa ctttataggt
     2401 aatagtttta tatcttatgt ttttaaggat ataaatctat tggattattt agatgttatt
     2461 aaagatatta cttttgagga agttgaagaa agattaaaag agcattttaa agaagagtat
     2521 tgtgttattt ctattgtaga acctaaatag aaacttaatc ataataagaa taactaatta
     2581 agtattattt atgtttttaa atgtttattt tggtaaaaca aatttataaa atatatttat
     2641 ataaattgag gtaaaaattt aagagctata gataaggaat agaagaggtt gatgtatata
     2701 tgcatcaatc tttttgatta aaaaatatct atgtacactt gtaactttag tcatgagaga
     2761 ttcagtttaa aaaattttat agtaattata ttagtataat atagtgtagg gatttgtaga
     2821 ataaatttta atattttttg tagaattaac attaatgtgt tatcataatt tagtataatt
     2881 acaattaatt gtaattatag catattgctt tggaaatatt ttcaattaat acaaagaggt
     2941 gacgttatgg atagagtagc atttacactg tttggcatag acatcatgtg gtatggaata
     3001 cttatggcat gtggaatgat tttaggaact ttaatagcaa taaaagaagc aaaaagagtg
     3061 ggaattaaag atgatgatgt attaaatata gctataatag caattccagt tggactaatc
     3121 tgtgcaagaa tctattatgt tgtatttaat tggagttact atgctcaaaa tatgtctcaa
     3181 atttttaatt ttagaggtgg tggacttgct atacatggag ggttaattgg tggaatatta
     3241 gctggttata tatacactaa gattaaaaat attaacttct taaaaatggc tgatacagtt
     3301 atacttggaa tgcctttagc acaagctatt ggaagatggg gaaattttat taatggagaa
     3361 gcacatggag gtgctaccaa tttaccttgg ggaataatgg ttgatggagt aaaggtacat
     3421 ccaacttttt tatatgagtc catatgggat tttggaatat ttatagtttt attactattt
     3481 agaaaaaata aaaaatatga aggtcaagtt atagttactt atataacttt atattcaata
     3541 ggaagatttt ttatagaggg attaagaaca gatagtttaa tgttaggacc acttagaatg
     3601 gcacaagtta taagtctaat tggagttatt ggtggcataa ttgcacatgt ttatttatca
     3661 aagaaaaata aacataatat tagtgaagaa tagtttaaaa agtgaattta agggaggtaa
     3721 ctatggaatt tcatcatgta tcagttcttt taaatgagtg tatagagaat ttaaatataa
     3781 agccagatgg cgtgtatgtt gactgtacta tgggaggagc aggacattct aaagaaatag
     3841 taaaaaaact gtcagataag ggattattta tagggtttga ccaagataaa aatgctataa
     3901 gcacagcaaa agagagattg agtgaatatg aaagtagagt gaagtttgtt cacagcaatt
     3961 ttgaaaatat aaaagaggaa ttagaaaaaa taggagttta caaaattgat ggtgtattag
     4021 ctgatttagg agtttcatct catcaacttg atgaagctga tagaggattt tcttatatgc
     4081 aagatgcacc acttgatatg agaatggatg ttaggtgtga gttttctgca tatgatgttg
     4141 taaatactta tacagaagat gaattaacta agataataaa agattatgga gaagataact
     4201 gggctaagcg tatagctaaa tttatagttg aagaaagagc aaacaaacca atagaaacta
     4261 caggaaaatt agtagatata attaaaaaag ctatccctaa gaaagctaga atagatggac
     4321 ctcacccagc taaaagaact tttcaggcaa tacgaataga agtaaataat gaacttggag
     4381 taattacaaa gatgataaat gatgcttctt ctataatgaa taaaggtgga agaatatgta
     4441 taataacatt ccattcatta gaagatagaa tagtgaaaaa tgcatttaag catttagcat
     4501 cagattgtat atgtccacaa catttaccta tatgccaatg tgataaagaa tctgaggtga
     4561 aaataataac tagaaaacct atattaccta gtgaggaaga aatagaagta aatccacgtt
     4621 caagaagtgc taagctaaga gtagcagaaa aaatataagt agttataagg ctaaagaata
     4681 atcttatata taaaataaga ttactataat taattaaaaa gtattatata ttttgttgaa
     4741 ctggtaaagc aattaaaaaa caattaacac tataagaaat gttctttgtt attgagatat
     4801 ataatgaaaa tttagatata aaaata
//
LOCUS       DAMJWP010000245         4817 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.252, whole genome shotgun sequence.
ACCESSION   DAMJWP010000245 DAMJWP010000000
VERSION     DAMJWP010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4817
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.252"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            244..1338
                     /locus_tag="PC700_003256"
     CDS             244..1338
                     /locus_tag="PC700_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582525.1"
                     /translation="MSEKVNVKTVISFAGAYVATVIGSGFATGQEIMQFFSFYGFAGI
                     IGGIISMVLFSWMGASVMSKGKELQLKEPIKIYRVYCGKYLGIFFEWFGPLFLFGVFV
                     VMISGAGATLTEYYGLNPFVGRVGMAVVALLTVSLGLDKLSKILGGIGPIIIIFTLLV
                     GGISLAKNIGNIGEAAQVLSTVKIAKPVPNAYLSGVIYTTYNTIVVMAFLTGLGASAA
                     NKKEAICGGILGGVALMAAAIMMHLAILSDIGNLYSKAIPSLFLADKISPVIGVLFSI
                     ILILGIYTTAVPLLWSVTNRFVDDKHPKFKLITLIAAILGLIGGFLPFDKLVGILYPY
                     TGYMGVIILVCVLYRQITKTSGYQEGISDK"
     gene            1564..1980
                     /locus_tag="PC700_003257"
     CDS             1564..1980
                     /locus_tag="PC700_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582526.1"
                     /translation="MNVKFNIKGIIYGVIALVFVIGGFIFIPSMFVEKSKPIDYTVLQ
                     RNSIPEKILDVMDKYTNEERALAAKIDNKIYIIVTRGNNKYGIEMDKIESVVEEGKDV
                     LRVKIKYKDKEDSFPYIVVETNMSELPDRIELNATK"
     gene            complement(2162..2731)
                     /locus_tag="PC700_003258"
     CDS             complement(2162..2731)
                     /locus_tag="PC700_003258"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDF2582527.1"
                     /translation="MKKEFRKKVIESRKNKSANFVLSNSNLITEKLLQMEDIRNATNI
                     MLYLNFNNEVQTDNLIKKLLSLKKIVSSPITIKENHTLIPTQITDLKEGLRIGAYGIR
                     EPNEKSPTIDIKALDVVIVPAVAYDIHCYRLGYGGGFYDRFLENLRKDAITIGIAFDF
                     QIFDSIPKETHDAQLDYIVTETRIITPNN"
     gene            2885..4042
                     /locus_tag="PC700_003259"
     CDS             2885..4042
                     /locus_tag="PC700_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HDF2582528.1"
                     /translation="MYLVISKKKAIFLGVILVIITAMVTSAFQLTLGNKVVISKELYE
                     DYKKYDKLLGLESIIKQDFYKKVSDTDLVNGAAKGLFLGTNDKYSGYYTKDEMENLIN
                     DSEGSYVGVGMYIGASKDGGLVVVPMKDSPAEKAGVKSGDKLVKVNGKSVSYKNSDEA
                     VRMMKGKKGKTVELTILREDKQLNFKVKTDQIIEKSIESKVIDNDLGYIEITQFISST
                     YTDFDKALKELKAKNIKGLVIDLRNNPGGMLDICKEVADELIGEGTIVYTKDNKGNTE
                     YLKSDKEKLGLPIVVLTNGESASAAEILTAAIVDNKEGISVGTTTFGKGLVQSVVRLK
                     DGTGYKLTTAQYFTPNGDYINEKGIKPTIEEKDENKQLDVATKWLREQIDK"
     gene            4229..>4817
                     /locus_tag="PC700_003260"
     CDS             4229..>4817
                     /locus_tag="PC700_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2582529.1"
                     /translation="MFNKNEILKKLIEEYDVKTTTDIQDMLKDLFASTIHQMLEAELD
                     DHLGYDRYDNKNKNTKNSRNRYRNKNVKSDFGEITLNIPRDRNGDFEPKVIRNYENDI
                     SGIENQVIGMYSKGMSTRDISSHLKSIYGIDISHSLIAKITDRVLPLAHEWQSRALDM
                     VYPIVFMGAIHYKVRSEGRIINKAAYIAIGVNLEGI"
BASE COUNT         1818 a          516 c          855 g         1628 t
ORIGIN      
        1 aaaatatgac taatatataa tctatagaga aactaatttt atatataatg actacttaga
       61 taaaagaatc taaaaaaatt atgagaggag gagtagggta taaaatcaga ttataagttt
      121 taagttatta tactaaaaag ctataaaaca gttatcatgt attttatgtt aaaggaaatt
      181 gtaggtagtt tatcgaatta tttaatcaga agaaaaaatt tcacatgggg aggtaactga
      241 gatatgtcag aaaaagtaaa tgttaaaaca gtcatcagtt tcgcaggtgc gtatgtagca
      301 acagtaatcg gttctggatt tgcaacagga caggaaataa tgcaattctt ctctttttat
      361 ggttttgcag gtattattgg tgggataatt tcaatggtat tattctcatg gatgggtgct
      421 tcagttatgt caaaaggtaa agagcttcaa cttaaagaac ctatcaaaat ttatcgtgtt
      481 tattgtggta agtatttagg gatattcttt gaatggtttg gacctttatt cttatttggt
      541 gttttcgtag taatgatttc aggagcagga gcaactttaa ctgaatacta tggattaaat
      601 ccatttgtag gtagagtagg tatggcagta gtagcattat taactgtttc actaggtctt
      661 gataaattat caaagatact aggtggaata gggcctataa taataatctt tacactttta
      721 gttggtggta taagtttagc taaaaacata ggtaatatag gagaagctgc acaagttctt
      781 agcactgtaa agatagctaa accagttcca aatgcctatc tatctggtgt aatatatact
      841 acttataaca caattgttgt tatggcattc ttaactggat taggagcaag tgctgctaac
      901 aaaaaagaag ctatttgtgg tggtatatta ggtggagtag cacttatggc tgcagctata
      961 atgatgcact tagctattct ttctgatata ggaaacttat attcaaaagc aataccatca
     1021 ttattcttag cagataagat ttcaccagta attggagttt tattctcaat aattcttata
     1081 ttaggtatat atacaacagc tgtaccttta ttatggtcag taactaatag atttgtagat
     1141 gataaacatc ctaagtttaa attaataact ttaatagcag ctatattagg tcttataggt
     1201 ggcttcctac catttgataa gctggttggt attttatatc catatactgg atatatgggc
     1261 gtcataatac tggtttgtgt tctttataga cagattacaa aaacatctgg atatcaagaa
     1321 ggaatcagtg acaaataagt attttttaat attgtaaact caagagctat atgaaaacaa
     1381 tgaaaatgtt ttcatatagc tctttttatt ttttagctcg tttattatga aataggaaaa
     1441 acatgttttt tagttacatt acatatattt ataattatgg ataaatatat actaatcaat
     1501 aatttagagt ctaagataag cttataaatc atatatttag aatatatggg aggagtgata
     1561 agattgaatg taaaatttaa tattaaaggt ataatttatg gagttatagc tcttgtattc
     1621 gttatagggg gatttatatt cataccaagt atgtttgtag agaaatctaa acccatagat
     1681 tatacagtat tacaaagaaa ttcaatacca gaaaagatat tagatgtgat ggataagtat
     1741 acaaatgaag aaagagcatt agcggctaaa atcgataaca aaatatacat tatagtgact
     1801 agaggaaata ataaatatgg tatagaaatg gacaaaatag aaagtgtggt tgaggaagga
     1861 aaggatgttt tgagagtcaa gataaagtat aaagataagg aagattcttt tccatacata
     1921 gttgttgaaa caaatatgag tgaacttcca gatagaatag aattaaatgc gacgaaataa
     1981 gaaggtattt tataattaaa taactgcata ttgtacaata aagtcatggt ttttattaat
     2041 gtaagtaaat tgtatatggt tcttattgtt gtaaaagcca tgacttaaaa tgcttataaa
     2101 gtaagatatt aacagtagta tttattacta tattaaatgg ggtctggaaa tacttaatgt
     2161 tctaattatt tggagtgata attctagttt cagtaactat gtagtctaat tgtgcatcat
     2221 gagtttcttt tggaatagaa tcgaatatct ggaagtcaaa tgctatgcca atagttatag
     2281 catcttttct taagttttct aaaaatctat cataaaatcc tccaccatat cctaatctat
     2341 agcaatgtat atcatatgct acagctggta caattacaac atctaaggcc ttaatatcaa
     2401 ttgtaggtga tttttcgtta ggttctctta ttccatatgc tccaattctc aatccctctt
     2461 ttaaatctgt tatttgagta gggattaatg tatgattttc ttttattgtg atgggacttg
     2521 atactatttt ctttagagac aaaagttttt tgattaaatt atctgtttga acttcattgt
     2581 taaaatttag ataaagcatt atattagttg catttcttat atcttccatt tgaagtaatt
     2641 tttcagttat taaatttgaa ttactaagta caaagtttgc actcttattt tttcttgatt
     2701 caataacttt ttttctaaat tcttttttca taaaataatt cctcccttaa tatgttataa
     2761 tgtgtacagc aattgtattt aatataaatt ttagtataat ttattgtaat aaagcaatat
     2821 atttgggtaa atgtaataaa aataacagct agtcatataa taataatagt ttatgtaaaa
     2881 ggagatgtat cttgtgattt caaagaagaa agctattttt ttaggggtga ttttagtaat
     2941 tataacagct atggttacat cagcatttca gttgacattg gggaataaag ttgtaatatc
     3001 gaaagaactc tatgaagact ataaaaaata tgataagttg ttaggactag aatctattat
     3061 aaaacaagat ttttataaaa aagtatcaga tactgatttg gtaaatggag cagcaaaagg
     3121 tctatttcta ggaacaaatg ataagtattc tggttattat actaaagatg aaatggagaa
     3181 tctaattaat gatagtgaag gttcttatgt tggtgtaggt atgtatatag gggcatcaaa
     3241 agatggtggc ttagtagtag ttcccatgaa agattctcca gcagagaaag caggtgtaaa
     3301 atcaggggat aaattagtaa aagtcaatgg aaaatctgtc tcatataaaa attcagatga
     3361 agctgttcgt atgatgaagg gtaaaaaagg gaaaacagtt gaactgacta tattaagaga
     3421 agataaacaa ttgaacttta aagtaaaaac agaccagata attgagaaga gtatagaaag
     3481 taaagtaatt gataatgatt taggatatat agaaattacc caatttatat caagtacata
     3541 tactgacttt gataaagctt taaaagaatt aaaagcaaaa aatatcaagg gattagtaat
     3601 agaccttaga aataatcctg gtggaatgct agatatatgt aaagaagttg ctgatgagtt
     3661 aattggtgaa ggaactatag tatatacaaa agataataag ggaaatactg agtacttgaa
     3721 atctgataaa gaaaaattgg gacttccaat agtagtgtta actaatggag aaagtgcttc
     3781 tgctgctgaa atattaacag ctgcaatcgt tgataataaa gaaggtattt ctgtagggac
     3841 tacaactttt ggtaaagggt tggtacaatc agttgtaaga ttgaaggatg gaacaggata
     3901 caagttaact actgctcaat attttacacc taatggagat tatatcaatg aaaaaggcat
     3961 aaagcctact attgaagaaa aagatgaaaa taaacagtta gatgtagcga ctaaatggct
     4021 tagagaacag attgataaat aaagtttaat ttttataaac ttatattgta aaataaagaa
     4081 ccttaataaa aggagtgtct caaaattaac tttttagttt atgaggcact cttttgtggg
     4141 tctttaaaat aaagtgtgta ttcttctaaa aagtagtgat aattaaaata ttaaatatca
     4201 ctatatataa ggaaggtgca cactttttat gtttaacaaa aacgaaatat taaaaaaact
     4261 tattgaagaa tatgatgtta aaacaactac agatattcaa gatatgctaa aagatttatt
     4321 tgcaagtaca attcatcaaa tgttagaagc ggagcttgac gaccatttag gttatgatag
     4381 atatgacaat aagaataaaa atactaaaaa ctctagaaat agatatagaa ataaaaatgt
     4441 taaatcagat tttggagaaa taactttaaa tattccaaga gatcgaaatg gagattttga
     4501 accaaaggta attagaaatt atgaaaatga tatatcagga atagagaacc aagtgattgg
     4561 aatgtactca aaaggtatgt ctactcgaga tatatcaagc caccttaaga gtatttacgg
     4621 aattgatata tctcattctc taatagctaa aattactgat agagttctac ctttagctca
     4681 tgaatggcaa agcagagctc ttgatatggt ttatccaatt gtatttatgg gtgctataca
     4741 ttataaagtt agaagtgaag gtagaataat aaacaaagct gcatatatag ccataggtgt
     4801 taacttagaa ggtataa
//
LOCUS       DAMJWP010000246         4717 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.253, whole genome shotgun sequence.
ACCESSION   DAMJWP010000246 DAMJWP010000000
VERSION     DAMJWP010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4717
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.253"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            165..1316
                     /locus_tag="PC700_003261"
     CDS             165..1316
                     /locus_tag="PC700_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDF2582530.1"
                     /translation="MEIYLDNSATTKPYQEVIDKMVYALNTEYGNPSSVHRKGVEVEK
                     AIKEVRQDIAKSLGAKEKEIYFTSGGTECNNTIIRGITSLNKKRKNHIISTNIEHPSV
                     LNTLKDLEEDGFEVTYLEVGKDGKINIEDLKNAIKSTTCLVSMMHVNNEIGTIQPIGE
                     VGKYLKGLKEKIYFHVDAIQSYGKINFRPSKYNIDFMSVSAHKFHGPKGIGFMYIKEN
                     NRLKPMLTGGGQEIGIRSGTENVPGIYGLGEAVRILNKDLDVVISKVNNLKNILKNEI
                     IDNIEDIKINSPEDGVCHILNVSFRGTKGEVLLHYLEQKGIYVSTGSACSSKKKGSYV
                     LNAIGLTNEEINGTIRFSLSDMNTEEEMLEAVKVLKESICDLRSIMKRK"
     gene            1362..2537
                     /gene="thiI"
                     /locus_tag="PC700_003262"
     CDS             1362..2537
                     /gene="thiI"
                     /locus_tag="PC700_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDF2582531.1"
                     /translation="MVYNILIVKYGEIGVKGKNRYIFENRLIRNIRNMLKPIGRFNVY
                     KEYGRIYVDLEDYDYEEVIEEVRKVFGIVGVCPGVRAKKDYDTLKEIALKMLEEKIEA
                     GYKTFKVESRRGDKSFGLTSQEMSMDIGGYLLSKVGDRINVDVRNPEVKIKCEYREFH
                     TMVYSDTIPGYGGLPLGTNGRAMSLLSGGIDSPVATWMVAKRGMEVEAVHFHSYPFTS
                     ERSQEKVKDLAKILAKYCGRVRLHKVNILEIQKAIGENCNEEEATILSRRFMMRIAQR
                     LSEKRHCDALITGESIGQVASQTIQGLTCTNAVVDLPVFRPLIAMDKSDIVDIAKKIG
                     TFETSIVPEEDCCSVFSPRKPVTKPRLEKIEKSETALDIEKLVQDAIDKIEVEDIEF"
     gene            2693..3496
                     /locus_tag="PC700_003263"
     CDS             2693..3496
                     /locus_tag="PC700_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF2582532.1"
                     /translation="MNKKGFTLIELLVVISIIGILVIVAVPALFRNIEKSKAVTCLSN
                     RENIKTQIVIAMAEESSKDKNEVIKEVLENKDGKYFETEPKCKSGGIYSATFDDGYDG
                     ITGIESIAKVYVTCTKHPDGIEMARDIHQSMKDLIASFAQDPSIIPGASKGNDDFRKY
                     LLDNKYKNGWPTIPDEFKAKYGLSKDTLYIQPYAYNPTKSDATVVVFANNKTGGNWYT
                     SLVYDYDEGRWYKGKNGISVAGRSWDVDTDSVKSVKTEIHSKEGWGPLN"
     gene            3739..4380
                     /locus_tag="PC700_003264"
     CDS             3739..4380
                     /locus_tag="PC700_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HDF2582533.1"
                     /translation="MNNNFQDNQTINLIQSRRSIRKFTTEQISDEQVNTLLHCAFAAP
                     SGCNKQPWHITVVQDQKLLKEISDDTLSRIHEVSNVEINKNFKLFYGAPTVLFISYDE
                     SSSWAPYDIGILTGNITTAAQALGLGSCIIGMVRGLFTPVEQGDIEGLVSVLDKEDVK
                     ESESIKMKFDTNKKYRELLDIPEGYSVPFGIAVGIPDGNLPNAREVVYKVSRV"
BASE COUNT         1892 a          448 c          913 g         1464 t
ORIGIN      
        1 ttttactata aaaatttgta ataaagtgaa aaaactagct tttcatgaaa taatatttta
       61 taatttaaga ttgtcttttt aataaagatt aacaaaaaaa atactataat tagtggtata
      121 atagttagag ttgaagattt taaataaagg taggtgtaat taagttggaa atatacttag
      181 acaatagcgc aactacaaaa ccttatcaag aagttataga taaaatggtt tatgcactta
      241 atacggaata tggaaatcct tcttctgtcc atagaaaagg tgtagaagtt gaaaaagcta
      301 taaaagaagt aagacaagat atagctaaat ctctaggagc gaaagaaaag gagatttatt
      361 ttacatcagg tggtacagag tgtaataata ctataataag aggtattact agtttaaata
      421 aaaaaagaaa aaatcacata atatctacta acatagaaca tccttctgtt ttaaatacat
      481 taaaagattt agaagaagat ggttttgaag taacttattt agaggtcgga aaagatggga
      541 aaataaatat agaggattta aaaaatgcga taaagtcaac tacatgctta gtaagcatga
      601 tgcatgttaa caatgaaata ggaactatac agccaatagg tgaagttgga aagtatttga
      661 agggtttaaa agaaaaaatt tatttccatg ttgatgctat tcagtcatat ggaaaaatta
      721 attttagacc atctaaatac aatatagatt ttatgagtgt aagtgcacat aaatttcatg
      781 gtccaaaggg aattggattt atgtatataa aagaaaataa tcgccttaag cctatgttaa
      841 caggtggtgg tcaggaaatt ggaattaggt cgggaacaga aaatgtacct ggaatatatg
      901 gtcttggaga agctgttaga atactaaaca aagatttaga tgttgttatt tcgaaagtaa
      961 ataacttaaa aaatatatta aaaaatgaaa taattgataa tatagaagat ataaaaatca
     1021 attctccaga agatggagta tgtcatatat taaatgtatc atttagaggg acaaaaggag
     1081 aagttttact tcattaccta gagcaaaaag gtatttatgt atcaactggc tctgcatgtt
     1141 cttctaaaaa gaaaggtagt tacgtattaa atgctatagg tcttacaaat gaagaaataa
     1201 atggaacaat aagatttagt ttatctgata tgaatacaga agaagaaatg ttagaagctg
     1261 tgaaagtttt gaaagaatca atttgtgact taaggtcaat aatgaaaaga aaataaagaa
     1321 tactgtatag atagtgataa taaattagtt taagaggtga aatggtgtat aatatattaa
     1381 tagtaaagta tggagaaatt ggagtaaaag gaaagaatag atatatattt gagaataggc
     1441 ttataagaaa tataagaaat atgttaaaac caattggaag atttaatgta tataaagagt
     1501 atggaagaat atatgttgat ttagaagact atgattatga agaagtaatt gaagaagtta
     1561 gaaaagtatt tgggatagtg ggtgtgtgcc ctggagtaag agctaaaaaa gattatgata
     1621 cattaaaaga aatagcttta aaaatgttag aagaaaaaat agaggctgga tataaaactt
     1681 ttaaggtaga gtcaagaaga ggagataagt ctttcgggtt gacttctcaa gaaatgagta
     1741 tggatatagg tggatactta ttatcaaagg taggagatag gattaatgtt gatgtaagaa
     1801 atccagaagt taaaataaag tgcgaataca gagaatttca tactatggtc tatagtgata
     1861 ctatacctgg atatggtgga ttgccacttg gaacgaatgg tagagctatg tctcttttat
     1921 caggaggtat agatagtcca gtagctacat ggatggttgc aaaaagaggt atggaggtag
     1981 aggcagttca cttccatagt tatccattta caagtgaaag gtcacaggaa aaagttaagg
     2041 atttagctaa aatactggca aaatactgtg gtagagtaag acttcataaa gttaacatat
     2101 tagaaataca aaaagcaatt ggtgagaatt gtaatgaaga agaagctact attctttcaa
     2161 gaagatttat gatgaggata gcacagagac tttctgaaaa aagacattgt gatgctttaa
     2221 ttacaggtga aagtattggg caggttgcat ctcaaactat acaagggctt acttgtacta
     2281 atgccgttgt agacttacca gtgtttagac cacttatagc aatggataaa tcagatatag
     2341 ttgatatagc caaaaaaata gggacttttg aaacatctat agtgcctgaa gaagattgtt
     2401 gtagtgtatt ttctcctaga aagcctgtca ctaaacctag attagagaaa atagaaaaat
     2461 ctgaaacagc acttgatatt gaaaaattag tacaggatgc tattgacaaa atagaggtag
     2521 aggatattga gttttagcat acaagttatt taattactaa attgaatatt gagtatatta
     2581 gtagttctgt aagtatatta gtagttctgt tgaaaggatt tatgtagaca atttattaat
     2641 atatagtatt taaaaataag tactataatt ttatttaatg ggaatgatta ttatgaataa
     2701 aaagggtttt acattaattg aattgttggt agttatatct ataataggaa ttttagttat
     2761 agtagctgtt ccagcgttat ttagaaatat agaaaaaagt aaggcagtta catgtctttc
     2821 taatagagaa aatataaaga ctcaaattgt tattgcaatg gctgaggaat caagtaaaga
     2881 caagaatgaa gtcataaaag aggtattaga aaacaaagat ggtaagtact ttgaaacaga
     2941 accaaagtgt aagtcaggtg gaatatattc agcaacgttt gatgatggtt atgatggaat
     3001 aactggaata gaaagcattg caaaagtgta tgttacttgt acaaaacatc cagatggtat
     3061 tgaaatggct agggatatac atcaaagtat gaaagatttg attgcatcat ttgcacaaga
     3121 cccttctata ataccaggag cttcaaaggg caatgatgat tttagaaaat atttattaga
     3181 caataaatat aaaaatgggt ggcctacaat tccagatgaa tttaaggcaa aatatggatt
     3241 aagtaaggat acactatata tacaaccata tgcatataat cctactaaat ctgatgctac
     3301 tgtagttgta tttgcaaata ataagactgg aggtaattgg tatacttccc tagtttacga
     3361 ttatgatgaa ggtagatggt ataaaggtaa aaatggtatt tctgttgcag gtaggtcatg
     3421 ggatgttgac acagatagtg ttaagtctgt aaaaacagag attcattcta aagagggatg
     3481 gggtccttta aattaatata tcatgtttta ttaatctggt attgattaaa ctatagaaaa
     3541 gagcagtttc tatagatgac gaataccaga ttttcattta tatgtataat taagatatat
     3601 taaaagtatt tatattttta agtcttcaca tacaagctaa agataattat aataatattg
     3661 taaagaaatt taattataag attaattttc tgaagattta gtatttgtac taagaaatac
     3721 ataaaaaggg gaatagatat gaataataat tttcaagaca accaaacaat aaatttaata
     3781 caaagtcgta gaagtatacg aaaatttaca acagagcaaa ttagtgatga gcaagtaaac
     3841 acattattac actgtgcatt tgcagcacct tcaggatgta ataaacagcc ttggcacata
     3901 actgttgtgc aagaccagaa acttttaaaa gagattagtg atgatacttt atctaggatt
     3961 catgaagtga gtaatgttga gataaataaa aactttaagt tattttatgg tgctccaacc
     4021 gttttattta tttcttatga tgaaagtagt tcatgggctc catatgatat aggaatactt
     4081 acagggaata taacaacagc tgcacaagca ctagggcttg gaagttgtat aataggaatg
     4141 gttagaggtt tatttacacc tgttgaacaa ggggatattg aaggtcttgt gagtgtattg
     4201 gataaagaag atgtaaagga atcagagtca ataaagatga agtttgatac aaataaaaaa
     4261 tacagagaac tacttgatat accagaaggg tattctgtac catttggtat tgctgtaggt
     4321 attccagatg gaaatttacc aaatgctaga gaggtagttt acaaagtttc cagagtttag
     4381 gataaagtat tgcaagaacc aatcagttac ttgaagaatt aaaccagtaa tatacaaata
     4441 aattgatgga agaggaagct atttaaaaag tagtttagat ttttcattgt aaaataatta
     4501 aataaagaat aaggtgataa gtattttatt tattatctta ttctttttta tttttgtttt
     4561 gtagaatgat aaaaataagg attaagtgta ataaaataga aaacaagtat gttaatttta
     4621 agtatatgct ttaaaattta tccaagttaa aaatttaaga tatcttttca gtataatgga
     4681 aaaaatgtca aaatattgaa atagggaaaa aatagtg
//
LOCUS       DAMJWP010000247         4703 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.254, whole genome shotgun sequence.
ACCESSION   DAMJWP010000247 DAMJWP010000000
VERSION     DAMJWP010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4703
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.254"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            138..1424
                     /locus_tag="PC700_003265"
     CDS             138..1424
                     /locus_tag="PC700_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HDF2582534.1"
                     /translation="MKLELGNIFIKNVEFGEKTEVKDGVLYISSEEIEKIVLEDERIV
                     SVNVELARPGESIRIAPVKDVIEPRVKIGDESKIFPGIINKVKTVGSGRTHVLLGACV
                     VTCGNIVGFQEGVIDMSGPTAKYTPFSKTNNICIVIKAKDGIDTHDYEEAARIAGLKI
                     GAYVGEAGREVEPDEVIVYETKPLLKQVKEYPDLPTVAYVHMLQSQGLLHDTYYYGVD
                     AKQIVPTFMYPTEIMDGAIISGNCVAPCDKVTTFHHLNNPVIHDLYKRHGKDLNFIGV
                     ILTNENVFLVDKERSSDMVAKLIEFLGVDGVLVTEEGYGNPDTDLMMNCRKCSEVGAN
                     VVLITDEFPGKDGKSQSIADATKEADAVVSCGQGNLVVHFPAMEKIIGTLDYVEMMIG
                     GYKGCLNEDGSMDAELQIIIASTIANGYNHLTARYY"
     gene            1510..2817
                     /gene="grdB"
                     /locus_tag="PC700_003266"
     CDS             1510..2817
                     /gene="grdB"
                     /locus_tag="PC700_003266"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2557..2559,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HDF2582535.1"
                     /translation="MSKIRVVHYINNFFAGIGGEEKADIPPEKRAGVVGPGIAFQSQF
                     NDDAEIISTVICGDTYFGENIESATQELLKMIKEESPDLFIAGPAFNAGRYGVACGTI
                     CKAVEEELNIPVVTGMYKENPGVDMFKLDLHIVSTGNSAASLRKVVPIMTKLGLKLVR
                     GEEIGPPEEEGYIMRGIRKNFFHELRGSERAIDMLVKKMKGESFETEYPMPEFDRVTP
                     MKPVKDLSKIKLALVTSGGIVPIDNPDKIESSSATKYGIYDLTDMDSMSNKEFTTIHG
                     GYDRAYVLENPNLVVPLDVVRELEKEGVIGELVDYFITTTGTGTSVGNSKRFGEEFSK
                     KLLEDDVDAVILTSTUGTCTRCGATMVKEIERAGIAVVHICTVVPISLTIGANRIVPA
                     VGIPYPLGNPMLGEEESKKIRKNIVLKALNALTEDIEEQTVFE"
     gene            complement(3033..3257)
                     /locus_tag="PC700_003267"
     CDS             complement(3033..3257)
                     /locus_tag="PC700_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF2582536.1"
                     /translation="MTVYNLKLGQKGIIDNIAGNEKLMKRLLALGLIDGTEVEVKKIA
                     PLNDPIVIRFRGFDLAIRKSDAKNINLKNN"
     gene            complement(3354..4607)
                     /locus_tag="PC700_003268"
     CDS             complement(3354..4607)
                     /locus_tag="PC700_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HDF2582537.1"
                     /translation="MITLSFDIVQTLTLSIFFFLIGNLLKNKVNFLNNFCIPAPVIGG
                     LLFCFLNLFFKYFNIADISISGNLMPNFITFFFTTIGLEISINLIKKGGSVLFRYWIL
                     CGVLAFCQNILAITISKIIKLEPLLGLMCGNVSMEGGHGYSAAFGLTIENLGIEGAVG
                     VGLSAATIGLIMGGILGCPVAKFLINKYKLKPSSNIGLSIPRYNRNLKRFGSKFFRNK
                     NRIKNNNFSETITPSVFLEQVLLIFICINTGEIISRCFYITFNILLPSVVTCMFSAVI
                     FRNLNDKINILELNFKLIDFLKELSLGIFLTLSLMNIDLFELSTLLPPILLIVTFQVI
                     FIILFSIFICFRVLGKDFDSAIIISGLIGHGIGATPNALANMSSLTQKYGDSPKAFLV
                     VPLVSGFLLDAISIPCILFFINILT"
BASE COUNT         1830 a          571 c          822 g         1480 t
ORIGIN      
        1 aattaaaaat gaaaatttct atgacattaa tacatagaat atatatttta aataattggc
       61 atagaaaatg ctttatatat taataaacgt aataaatatt tttaattgcg attaaatata
      121 ttaaggggga ttttcatatg aagctagagc tagggaatat atttataaaa aatgttgaat
      181 ttggagaaaa gacagaagta aaagatggag tactatatat tagtagcgaa gaaatagaaa
      241 agatagtatt agaagatgaa aggattgtaa gtgtaaatgt tgaacttgca agacctggcg
      301 aatcaataag aatagctcca gttaaagatg taatagagcc tagagttaaa attggagatg
      361 aaagcaaaat atttcctgga attataaata aagtgaagac tgtaggaagt ggaagaaccc
      421 atgtactttt aggagcttgt gttgtaactt gtggaaatat agttggattt caagaaggcg
      481 taattgatat gagtggtcct actgctaaat atacaccttt ttcaaaaaca aataatatat
      541 gtattgttat aaaagcaaag gatggtatag atactcatga ttatgaagaa gcagctagaa
      601 ttgcaggatt aaaaataggt gcatatgtag gtgaggcagg aagagaagtt gagccagatg
      661 aagtcattgt atatgagaca aagccattat taaaacaagt aaaagaatat ccagacctgc
      721 caacagtagc atatgttcac atgttacaat cacaaggact tttacatgac acttattact
      781 atggggtgga tgctaaacag atagtgccta catttatgta tccaactgaa ataatggatg
      841 gagctataat atctggaaac tgtgtagcac catgtgataa ggttacaaca ttccatcatt
      901 taaataatcc agttatacat gacttatata aaagacatgg aaaagattta aactttatag
      961 gagttatttt gactaatgag aacgtattct tagtcgataa agagagaagc tctgatatgg
     1021 tagctaaatt aatagaattt ttaggagttg atggagttct agtgacagaa gaaggttatg
     1081 ggaatccaga tactgaccta atgatgaatt gtagaaaatg tagtgaagtt ggagcaaatg
     1141 tagttttgat aacggatgaa tttcctggaa aagatggcaa gagtcaatct atagcagacg
     1201 ctactaagga agcagatgca gttgtttctt gtggtcaagg aaatctagtg gttcatttcc
     1261 cagcaatgga aaaaattata ggaactttag attatgttga aatgatgata ggtgggtata
     1321 aaggatgctt aaatgaagac ggaagcatgg atgcagagct acaaattata atagcatcaa
     1381 ctatagcaaa tggctataat catttgacag caagatatta ttaaataagt atttgaatat
     1441 aaagttctgt tgatgtaatc gttggtctaa ttgatgtaat aactataaag tgatttggag
     1501 gattaagaga tgagtaaaat cagagtggtt cattatataa acaatttctt tgctggtata
     1561 ggtggagagg aaaaagcaga tattccgcca gaaaaaaggg ctggtgtagt aggacctgga
     1621 atagcatttc agagtcaatt taatgatgat gcagagataa tctcaactgt aatttgtgga
     1681 gatacgtatt ttggtgaaaa tattgagagt gcaactcaag aacttttaaa aatgataaaa
     1741 gaagaaagcc ccgatttatt tattgcaggc ccagcattta atgcaggtag atatggagta
     1801 gcttgtggga ctatttgtaa ggcagttgaa gaagagttaa atattcctgt agttacagga
     1861 atgtataaag aaaatccagg tgtagatatg tttaaattag atttgcatat agtgtctaca
     1921 ggaaattcag cagcaagtct tagaaaggta gttccaataa tgacgaaact aggtcttaaa
     1981 ttggttagag gtgaggaaat aggacctcct gaagaggaag gatatataat gagaggaata
     2041 agaaaaaatt tcttccatga acttagaggg tctgaaagag caattgatat gttggttaaa
     2101 aagatgaaag gggaaagctt tgaaactgag tatccaatgc ctgaatttga tagagttaca
     2161 cctatgaaac ctgttaaaga tttatctaag ataaaacttg ccctagtaac ttctggaggg
     2221 atagttccaa ttgataatcc agacaaaatt gaatcttcaa gtgctactaa atatggaatt
     2281 tacgatttaa cagatatgga ttctatgtca aataaagaat ttacaactat acatgggggg
     2341 tatgacagag catatgtcct tgaaaatcca aatttagttg taccattaga tgtagttaga
     2401 gagcttgaaa aagaaggtgt aattggagaa ttagtagatt attttataac tactacagga
     2461 acaggaacga gtgttggcaa ttctaagaga tttggagaag agttttctaa gaaattgtta
     2521 gaagatgatg tagatgctgt aattttaact tctacatgag gtacttgtac tcgttgtgga
     2581 gcaacgatgg taaaagagat tgagagagct ggtatagcag ttgttcatat atgtacagta
     2641 gtaccaatat cacttacaat aggagctaat agaattgttc cagcagtagg gataccatat
     2701 ccacttggaa atcctatgtt aggagaagag gaaagtaaaa aaattagaaa gaatatagtt
     2761 ttaaaagctt taaatgcact aacagaggat atagaagaac aaacagtatt tgaataataa
     2821 aaatataata tagaaataaa aaaagatatc ataaaatagt ttttgaaaat tattttatga
     2881 tatctttttg ctatggatgg aatgaagaaa gcaattaagt aataaaaata tttatagtaa
     2941 aacagccaat taaagtataa agaatgaaaa aaataaatag aagattatac ataaacaaaa
     3001 aaagagattt taaaatctct ttttattata tattaattat tttttaaatt tatatttttt
     3061 gcatcagatt ttctaatagc taagtcaaac cctctaaatc taatcactat agggtcatta
     3121 agaggagcta tctttttcac ttcaacttca gtgccatcta ttagtcctaa ggctagaagt
     3181 ctcttcataa gtttttcatt tccagctata ttgtctataa ttcccttttg tccaagtttt
     3241 aagttgtaaa cagtcattat taacacctcc taatgaaaat cattatcaaa ctttatattt
     3301 ataatatata acaaaaagag taaattgtca atatataaca aaaaataaac atactaagtt
     3361 agtatgttta taaaaaatag tatacatggg atactaatag catctagcaa aaaaccactt
     3421 actaatggta caaccaaaaa ggcttttgga gaatcaccat atttttgtgt caaagaactc
     3481 atatttgcca aagcgttcgg agtagctcct attccatgtc caatcaaacc acttattatg
     3541 atggcagaat caaaatcttt tccaagtact ctaaagcaaa taaatattga aaataggatt
     3601 ataaagatta cttgaaaagt tactattaag agaataggag gtaataatgt agataattca
     3661 aataaatcta tattcataag tgataaagtt aaaaaaattc ccaatgaaag ttcttttaaa
     3721 aaatctataa gtttaaaatt taattctaat atatttatct tatcatttaa atttctaaaa
     3781 ataacagctg aaaacataca agttacaaca gagggtaata gtatattaaa cgttatatag
     3841 aagcatctac ttataatttc tcctgtattt atacaaataa atatgagaag aacctgttcc
     3901 aaaaatacag atggtgttat tgtttcggag aagttattat tttttatcct attcttattt
     3961 ctaaaaaatt tacttccaaa tctctttaga tttctattat atctaggaat cgacaagcct
     4021 atatttgatg atggttttaa tttatattta tttattagaa atttagctac aggacatccc
     4081 aatataccac ccataataag tcctattgtt gcagcagata atccaacccc aactgctcct
     4141 tctataccga gattttctat tgtaagtcca aatgcagcag aataaccatg accaccttcc
     4201 atagatacat ttccacacat aagccctaaa agaggttcaa gttttataat ctttgatatt
     4261 gtaatagcta atatattttg acaaaatgcc aatactccac acaaaatcca atatctaaat
     4321 aatacactac cacctttctt tataagattt atgcttattt caagtcctat agttgtaaaa
     4381 aagaaggtta taaaatttgg cattaaattt ccacttatag atatatctgc tatattaaaa
     4441 tatttaaaaa ataaatttag aaaacaaaat aatagacctc ctattactgg tgcaggtata
     4501 cagaaattat ttaaaaagtt aactttattc ttcaataaat taccaattaa aaagaagaat
     4561 atagatagtg tcaaggtttg cactatatca aaacttaagg taatcaaaat aagtcctcct
     4621 ttgtaattca aagttttaat tatataactt aaattcttat gtattgaaat tttattatca
     4681 aatgtatatt aataaatgta tat
//
LOCUS       DAMJWP010000248         4681 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.255, whole genome shotgun sequence.
ACCESSION   DAMJWP010000248 DAMJWP010000000
VERSION     DAMJWP010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4681
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.255"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            37..1344
                     /locus_tag="PC700_003269"
     CDS             37..1344
                     /locus_tag="PC700_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582538.1"
                     /translation="MKEIADAITSEYLSYGINEDISIYEGRFCIYLDKKYRCNGKIYY
                     KMTPPISISFKADIGCVEEIDNEDDNLVLDYDNAILEVHGYKIISITINTLSEFSVEG
                     YINDDCIKSKNSYVEYVDFNIINLDKIPGKLIKYNDKVYAGRIEFDINDYVVTIDKRY
                     DYRKELKSELKSKSGAIITHIGRIRRKDGRIFRTNNTINLLDRISTALSFMCGRYVGF
                     CLAKGYRSGNEVYRIWNENQISPFRYVPTWSDTLSNYHNMEKYISLMCKKLEDFYYGS
                     AIKSVVDWYIESLGSATMENNIISVQIALETLSYVILVEQSKILTDEAFDCNLASKNI
                     RLLLDTCKIPYGKHELNIFDNIIKNKFDDGVDLVIYLRNSIVHPSRKTHRAVLEVEDI
                     WNIISIGTRYIELVLLFILGYRGEYSNRLVERCYGEVEVVPWN"
     misc_binding    1430..1692
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1818..2621
                     /locus_tag="PC700_003270"
     CDS             1818..2621
                     /locus_tag="PC700_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2582539.1"
                     /translation="MKNILKKVGIFTIMLGLLGGVVGCSKPDNEKDKDASKESKKEVV
                     VGFDNTFVPMGFLDEKGDTVGFDVDLAKETFKRLGMEVKFQPIDWSMKETELNDSKTV
                     DVLWNGYSITDERKKIVSYTEPYLQNKQIIVTLSDSKINSKADLKDKEVGTQQGSTAL
                     DAVEKDKDFMNSLKGGAPVLYDTYDKALRDLEIGRTSAVVGDEVLIRYYMGQKGEDKY
                     KVLKDDFGLEDYVVATSKENPELCEKINKTLKEMKKDGTFDKIYDKWFK"
     gene            2659..3270
                     /locus_tag="PC700_003271"
     CDS             2659..3270
                     /locus_tag="PC700_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDF2582540.1"
                     /translation="MLQGLEIVIAMFCITLIVSIPLGIGVAFLRLSKNKLVSGITQCY
                     ILIMRGTPLLLQMIVIFYGLPLLGIVFDRFTAGVVAFFLNYAAYFAEIFRGGIQSIDR
                     GQYEASKVLGFDKFTMYKRVIFPQVFKRILAPISNEVITLVKDTSLVYILGLNDILRI
                     SQIAMNREASLLPLFEAGAIYLIFVAILTKGFELLEKKYSYYR"
     gene            3302..3973
                     /locus_tag="PC700_003272"
     CDS             3302..3973
                     /locus_tag="PC700_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDF2582541.1"
                     /translation="MLKIKNLNKSFKKNRVLKDISFELEEGQIGVLLGKSGAGKTTIL
                     RCINGLEEFDSGEIIIDNEVIKNKRDMAKIRGKIGMVFQNFNLFPHMTVLENIIESPV
                     NVFKVPRKEAEERARELLRLVDLEDKLNSYPFELSGGQQQRVAIARSCALMPKVLCFD
                     EPTSALDIDTIQRVVNIMNRLKDKGMTILIITHDVVFSNNVADKIISIKDGIVENVQI
                     KEKIV"
     gene            4272..4634
                     /locus_tag="PC700_003273"
     CDS             4272..4634
                     /locus_tag="PC700_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HDF2582542.1"
                     /translation="MIKLYGYTKCSTVKKAKNWLKENNLEFEDIDMVQNPPSKEELKS
                     IYKTSGYDIKKFFNTSGMKYRELGLKDIVKTESDDKLLEILVSDGMLIKRPLLLDGKN
                     VLLGFKEDVWKSTLLKED"
BASE COUNT         1871 a          426 c          861 g         1523 t
ORIGIN      
        1 attgagtttt acataaaagc gaaggtggga ataaaaatga aagaaattgc tgatgctata
       61 acatcagagt atttaagcta tggtatcaat gaagatatta gtatctatga aggtagattt
      121 tgcatatatt tagataaaaa atatagatgt aatgggaaaa tttattataa aatgacacct
      181 ccaatatcta taagttttaa agcagacata ggttgtgttg aagagataga caatgaagat
      241 gacaatttag ttttggatta tgataatgct attcttgaag ttcatggata caaaattatt
      301 tctataacaa taaatacttt aagtgaattt agtgtagaag gttatatcaa tgatgactgc
      361 ataaaatcta agaactccta tgttgaatat gtagatttta atataataaa cttagataaa
      421 atacctggaa aactaattaa atacaatgat aaggtatacg caggtagaat tgaatttgat
      481 ataaatgatt atgttgtaac tatagacaag cgatatgatt atagaaaaga attaaaatca
      541 gaattaaagt ctaaatctgg agcaataata actcatatag gtaggattag aagaaaagat
      601 ggtagaattt ttagaacaaa taatacaata aatcttttag atagaatatc aacagctttg
      661 agttttatgt gtggaagata tgtagggttt tgtttggcca aagggtatag aagtggtaat
      721 gaagtttata gaatatggaa tgaaaatcaa atatctcctt ttagatatgt accaacatgg
      781 tcagatacac tttcaaatta tcataatatg gaaaaatata taagccttat gtgtaaaaag
      841 ctagaagatt tttattatgg ttctgctata aagagtgttg ttgattggta catagaatct
      901 cttggaagtg ctaccatgga aaacaatata atatctgtac aaattgcact tgaaactttg
      961 tcatatgtaa ttctagtgga acaaagtaag atacttacag atgaagcatt tgactgtaat
     1021 ttggcatcta aaaatataag actcttactt gatacatgta agataccata tggtaaacat
     1081 gaattaaata tatttgataa tataataaaa aataaatttg atgatggagt ggatttagtt
     1141 atttatttaa gaaatagcat agttcatcca agtagaaaga ctcatagagc tgtgttagag
     1201 gttgaagata tttggaatat aatttcaata ggaacgagat atattgaact tgttttactc
     1261 tttattttag gttatagagg agaatattca aacagattag tagaaaggtg ttatggtgaa
     1321 gtggaagttg taccttggaa ctagattttt atatttattt taaaatatat attgacatat
     1381 attgccttat gctataaaat caatatcata ataataattc agaataacat taaatgctaa
     1441 gaaaagaaat agtaaaggta gttaatctta aacagagagt ttccgattgg tgagaggagc
     1501 aatagatttt actttgaaca catctttgag caggactctg aaagagtaga aatactttat
     1561 taggagattt ccggaatcct tgcacccgtt atagtgctag agtataataa ttttattgta
     1621 cttaaagagg ttagtgtcgt gaggcattaa tgaacagagg tggaaccacg agagttaact
     1681 cccgtcctct agcagaagct agaggcggga gttttttaat ataatttttt aagcgcgcag
     1741 agaattatat tgcaatagat acacttttta aaaaataatc aaaatatttt aaaaattaaa
     1801 aggcgaggga gaatattatg aaaaatatat taaaaaaagt aggaatattt acaattatgt
     1861 taggtttatt aggtggagtt gtaggttgtt caaaaccaga taatgaaaaa gataaggatg
     1921 cttcaaagga atctaaaaaa gaagtagttg tagggtttga taatacgttt gtaccaatgg
     1981 gatttttaga tgaaaaggga gatacagtag gatttgacgt agacttagct aaagaaacat
     2041 ttaaaagact tggaatggaa gttaagtttc agcctataga ttggtcaatg aaggaaactg
     2101 aattaaatga ttcaaaaact gtagatgtat tatggaatgg atattctata acagacgaaa
     2161 gaaagaaaat agtatcatat acagaaccat atttacaaaa taaacagata atagttactc
     2221 taagtgattc aaaaataaat tcaaaagctg atttaaaaga taaagaagtt ggaacacaac
     2281 aaggttcaac agcacttgat gcagtggaaa aggataaaga ttttatgaat agcttaaaag
     2341 gtggagcacc agttctttat gatacttatg ataaagcact tagagattta gagataggta
     2401 gaactagtgc agttgttggt gatgaagttt taattagata ttatatggga caaaagggtg
     2461 aagacaaata caaggtatta aaagacgatt ttggattaga agattatgtt gtagcaactt
     2521 ctaaagaaaa tccagaactt tgtgagaaaa taaataaaac attaaaagaa atgaaaaaag
     2581 atggaacttt tgacaaaatt tatgataagt ggtttaaata gaagataagt tagctgttaa
     2641 gaagggggca ctaaaggcat gttacaaggt ttagagatag ttatagcaat gttttgtata
     2701 acattgatag tatcaattcc attggggatt ggagttgcat ttttaagatt atcaaagaat
     2761 aaattagtaa gtggtattac acaatgttat atattgataa tgagaggtac acctttactt
     2821 ttgcaaatga tagtcatatt ttacggatta cctcttttag gaattgtatt tgacagattt
     2881 acagctggag tagtagcttt cttcttaaat tatgcagcat attttgcaga aatatttaga
     2941 ggtggaatac agtcaatcga tagaggacaa tatgaagctt caaaagtact aggatttgat
     3001 aaatttacta tgtataagag agtaatcttt ccacaagttt ttaaaagaat actagcacct
     3061 atttcaaatg aagtaatcac attagtaaaa gatacatctt tagtatatat attaggatta
     3121 aatgatattt taagaatatc tcaaatagca atgaacaggg aagcaagtct gttaccatta
     3181 tttgaggcag gagcgatata tcttatattt gtagctatac ttactaaggg atttgaatta
     3241 cttgaaaaaa aatattctta ttatagataa agttacaata ttataggagg aggtttttta
     3301 tatgttgaaa atcaaaaact taaataaatc atttaaaaaa aatagagtac ttaaagatat
     3361 ttcttttgag ttagaagaag gtcagattgg agttttactt ggtaagtctg gtgcaggaaa
     3421 gaccacaata ttaagatgta taaatggtct tgaagaattt gatagtggag agattataat
     3481 agataatgag gtaataaaaa ataagagaga tatggcaaaa atacgaggaa aaataggtat
     3541 ggtttttcaa aatttcaact tatttcctca tatgacagtc cttgaaaata taattgaatc
     3601 cccagttaat gtttttaaag ttcctagaaa agaggctgaa gagagggcta gagaactttt
     3661 aagattagtt gatttagaag ataaattaaa ttcttatcca tttgaattgt caggaggaca
     3721 gcaacagaga gttgctatag ctaggtcatg tgcattgatg ccaaaagtcc tttgttttga
     3781 tgaacctact tctgcacttg atattgatac tattcaaaga gtagtcaata taatgaatag
     3841 acttaaagac aaaggtatga caatattaat aattacacat gatgttgtct tctcaaataa
     3901 tgttgcagat aaaattataa gtataaaaga tggtatagta gaaaatgttc aaataaaaga
     3961 aaaaattgtt tagagtttta taaatataaa ctagggtaga attttataat ataaataaag
     4021 ggcaagtaaa atcaaatata ctatgccctt ttgttatctc aaaatttaat cttatatttt
     4081 atgtttaagt tacattataa tttataataa aaataaaagg actatttaaa tatataatgt
     4141 gaataaatgt ttaataattt gaaatatgat aattttttat gttgaaagct tataatgatt
     4201 atgatataaa ttggttatag tatacaattt atgggtataa caataaaaaa ttagaaagga
     4261 atttttataa aatgattaaa ttatatggat atacaaaatg ctctactgtt aaaaaagcta
     4321 aaaattggct taaggaaaat aatttagaat ttgaagatat agatatggtt caaaaccctc
     4381 ctagtaaaga ggagttaaaa tcaatttata agactagtgg atatgatata aagaaatttt
     4441 ttaatacaag tggtatgaaa tatagagaat tgggtcttaa agatattgta aaaactgaat
     4501 ctgatgataa acttttagaa atattggtaa gtgatggtat gcttattaaa agaccattac
     4561 ttttggatgg taaaaatgta ttattgggct ttaaagaaga tgtatggaaa agtactttgc
     4621 taaaagaaga ttaggaagag ataggtgtgt tgattaaaat tattaattaa tacacctata
     4681 a
//
LOCUS       DAMJWP010000249         4680 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.256, whole genome shotgun sequence.
ACCESSION   DAMJWP010000249 DAMJWP010000000
VERSION     DAMJWP010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4680
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.256"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            3..401
                     /locus_tag="PC700_003274"
     CDS             3..401
                     /locus_tag="PC700_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2582543.1"
                     /translation="MIKKIEHVVITNNNIENYINFYKKFGFSSKEMSEKYKLKARNVK
                     INLNIKGYKFLPYVKNLKASRDNLCFKINTNIDEPKKYLEQNEIQIKRGIVECTGVFG
                     NMKSIYLKKTNGNLIELSNHRITESKPWKI"
     gene            389..1327
                     /locus_tag="PC700_003275"
     CDS             389..1327
                     /locus_tag="PC700_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDF2582544.1"
                     /translation="MENLILSINVVLPLFLTMSLGYVLKKLGLFDTHTLNKMNNVCFK
                     SFLPLLLFFNIYNTDLDGSLNFKLMAVAVIIIFSIFLILVFIIPKIEKDNKTRGALIQ
                     GVFRSNFVIFGIPVTISLFGDKSIGVTSLLIAVIVPMFNVLSVVILEIFRGGKVNIRH
                     MIKSVLTNPLIIASFIGLTMLILNINIPTFLHKTIGDISKIATPLSLIILGGSFSFSA
                     IKGHIKTIILGVCGKLILVPSIFLPISIFLGFRNVELTSLMIMLSAPTAVSSFTMAQQ
                     MDSDSELAGQIVVFTSGFSIITVFLIIFILKQNNLI"
     gene            complement(1711..1935)
                     /gene="secG"
                     /locus_tag="PC700_003276"
     CDS             complement(1711..1935)
                     /gene="secG"
                     /locus_tag="PC700_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDF2582545.1"
                     /translation="MSNILMGVQVVLGILLVISIMPQDSKNALPSEFGGEGSQAYFKP
                     KGKQAFLARVTKITAVLFFINALALLIVKK"
     gene            complement(2053..2559)
                     /locus_tag="PC700_003277"
     CDS             complement(2053..2559)
                     /locus_tag="PC700_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2582546.1"
                     /translation="MNLEIIRATSENAIEMGFIHSCSWRKAYKGIIPDKIIDEFTSEK
                     RAEVFQSVIPKAEEEYYLFKVNGIPAGFASLNRSQEDNAPKCVGEIYSIYFHPDFWGT
                     SVTKKGLQFCIDRLSNLGYSYITIWVLKDNTRAINFYKKNGFTCDDFEKEIHIGKKLL
                     EVRYSKKI"
     gene            2644..3072
                     /locus_tag="PC700_003278"
     CDS             2644..3072
                     /locus_tag="PC700_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2582547.1"
                     /translation="MNPVKELNNLWHIVSMQMKTEMCSGKYPTIHGISLVELSILEIV
                     EQYPNSMMKEISEILELPKSTLTSAVKRLELKKFIKKNPCIKDKRRYNLVLTELGLKA
                     QQEHRDIEIIVFRNLLHQLDKDEIETFIRIFSKAVNKSKK"
     gene            complement(3192..4484)
                     /gene="eno"
                     /locus_tag="PC700_003279"
     CDS             complement(3192..4484)
                     /gene="eno"
                     /locus_tag="PC700_003279"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDF2582548.1"
                     /translation="MSVIELVYAREVLDSRGNPTVEVEVVLEDGAMGRAIVPSGASTG
                     AFEAVELRDGDKGRYLGKGVETAVANVNEIIAPEIEGMDAFDQPAIDAIMIELDGTPN
                     KGKLGANAILGVSMAVARAAADEIGLPLFQYLGGVNAKQLPVPMMNILNGGEHADNNV
                     DVQEFMILPVGACCFKEGLRMGAEVFHSLKKVLGEKGLACGVGDEGGFAPNLGSNREA
                     LELIVEAITKAGYKPGEDVMLGLDVAATEMYNKETKKYVLAGEGKELTAAEMVALYED
                     WSNNFPIITIEDGLDEEDWDGWKLLTERLGNKLQLVGDDLFVTNTERLEKGIENGVAN
                     SILVKVNQIGTITETLDAIEMAKRAGYTAVISHRSGETEDSTIADLAVAVNAGQIKTG
                     APSRTDRVAKYNQLLRIEEMVGEQARYCGLKSFYNLKK"
BASE COUNT         1592 a          749 c          568 g         1771 t
ORIGIN      
        1 ccatgataaa aaaaatagag catgtggtca ttactaataa taatatagaa aattatatca
       61 atttttataa aaaatttggt ttttcatcta aagaaatgag cgaaaagtat aaacttaagg
      121 ctagaaatgt taagataaat cttaacataa aaggttataa atttttacct tatgttaaaa
      181 atttaaaagc aagtagagat aacttatgtt ttaaaataaa cacaaatatt gatgagccaa
      241 aaaaatactt agaacaaaat gagattcaaa ttaagcgagg aattgtagaa tgtacaggtg
      301 tctttggtaa tatgaaatct atttatttga aaaaaactaa tggaaattta attgaattat
      361 caaatcatag aatcacggag agtaaacctt ggaaaattta atcctatcaa taaatgttgt
      421 tcttcctttg tttctcacaa tgtcacttgg atatgttcta aaaaaattag gtctatttga
      481 tactcatact ttaaataaaa tgaataatgt ctgcttcaag tctttcttgc cattattatt
      541 attttttaat atatacaata cagatttaga tggttctctt aattttaagc ttatggctgt
      601 agctgttata attatttttt caatcttctt aatcttagtc tttattattc ctaaaattga
      661 aaaagataat aagacaaggg gtgcattaat acaaggtgta tttcgtagca atttcgttat
      721 atttggtatt cctgtgacaa tttcactatt tggcgataag agtattggag tcacatcttt
      781 attaattgca gttattgtac ctatgtttaa tgtattatct gttgtaattc ttgaaatttt
      841 tagaggtggc aaagtaaata taagacatat gattaaaagc gtcttaacaa atccactcat
      901 aatcgcctct tttataggat tgacaatgct aattttaaat attaatatac ctacattttt
      961 acacaaaact attggtgata tttctaaaat agctactcct ttatcactta taattctagg
     1021 cggatctttt agtttttctg ctataaaggg gcatataaaa accattatat tgggcgtatg
     1081 tggaaaactt atacttgtac cttctatatt tttaccaatt agcatttttc ttggatttag
     1141 aaatgtagaa ttaacttcac ttatgataat gttatctgct ccaactgcag tttcttcatt
     1201 tacaatggca caacagatgg attctgacag tgaattagct ggacaaattg tagtatttac
     1261 ttctggattt tctataatta cagtattttt aataatattt attttaaagc agaataatct
     1321 tatttaatga tatttgataa cacaaattgg tattatgaga aacatatata aaattgattt
     1381 aattgcgtaa aaacatatta ttatactatt tttactatat aatataactt tgtgtatatt
     1441 atctcattaa acatggtgaa agtagtgttg ctatatattt tttagtttga aaaaaatata
     1501 taaatataaa caatatataa atacaaataa taaatgccaa caaatagtca atctataaaa
     1561 taaaaactgg actaatcata taaaatcatt agtccagctt ttattttacg tactttttat
     1621 gttgatgtac ttttattaaa aactaaattt aatttttaaa tatctagcaa atgcactaat
     1681 taaataatgg ccagttattc aacaatatca ctacttctta actataagta atgccaatgc
     1741 atttataaaa aataatactg ctgttatctt tgtaactcta gcaagaaatg cttgttttcc
     1801 ttttggttta aagtaagctt gacttccttc tccaccaaat tctgaaggta aagcattttt
     1861 tgaatcttga ggcattatgc taattactag taaaatccct agcactactt gcacacccat
     1921 taaaatgttg ctcattattg cccctcctat ctatttatta cttaaatttc attactcttt
     1981 agtgtatcac aataatatag tcatttcaag actattgaag tataaataac aaacaaaaat
     2041 aatatgcaat tactatatct tttttgaata acgtacttct aataattttt ttccaatatg
     2101 tatctctttc tcaaaatcat cacaagtaaa accatttttt ttatagaaat ttatagctct
     2161 agtattgtct tttagaaccc atatcgttat atatgaatat cctaaattgc ttaatctatc
     2221 aatgcaaaat tgtaatcctt tttttgtcac agatgttccc caaaaatcgg gatggaaata
     2281 tatagaatat atttctccaa cacattttgg agcattatcc tcttgacttc tatttaatga
     2341 agcaaaacca gcaggtatac cattcacttt aaaaagataa tattcctctt cagcttttgg
     2401 aataacactt tgaaaaactt ctgctctttt ctcagaagtg aattcatcta ttatcttatc
     2461 tggaataatt cctttatacg cttttctcca cgaacaagaa tgtataaaac ccatttctat
     2521 agcattttca cttgtagctc gtataatctc taaattcata aaaacaccat cctattgaca
     2581 aattaatacg attatcgtac tatattagtg cgataattgt atcatatatg gaggtaaaag
     2641 tcaatgaatc ctgtaaaaga gctaaacaac ctgtggcata ttgttagtat gcagatgaaa
     2701 acagaaatgt gtagtggtaa gtatcctact attcatggta tttctttagt agaattaagt
     2761 attttagaaa tagttgaaca gtatcccaat agtatgatga aagaaattag tgaaatttta
     2821 gaattgccaa aaagcacatt aacaagtgct gtaaaacgtc ttgaattaaa aaaatttatt
     2881 aaaaagaatc cctgtattaa agacaaaagg aggtataact tagtcttaac tgaattaggt
     2941 ttaaaggctc aacaagaaca tagagacata gaaataattg tatttagaaa tctcttacat
     3001 caattagata aagatgaaat agaaactttt attcgtattt tttctaaagc tgtgaataaa
     3061 agtaagaaat gaaatataaa aaaaggataa gtgaattgaa aaatcaacta tcacttatcc
     3121 cttataagat aaatttttat cttttatgtt atctttttaa tctttaatgc catattttta
     3181 atcttcaata actatttttt taaattatag aaagatttta atccacagta tctagcttgt
     3241 tctccaacca tttcttctat tcttaataat tggttgtact tagcaactct atcagttctt
     3301 gatggagcac cagtttttat ttgaccagca tttactgcaa cagctaaatc tgctatagta
     3361 gaatcttcag tttctcctga tctgtgagat ataacagcag tatatcctgc tctcttagcc
     3421 atttctatag catctaaagt ttcagttatt gtacctattt gattaacttt aactaatata
     3481 gagttagcta ctccattttc aattcctttt tctaatctct ctgtgttagt aacaaataag
     3541 tcgtcaccaa ctaattgtaa tttatttcct aatctttcag ttaataactt ccatccgtcc
     3601 caatcttctt cgtctaaacc atcttctata gttattattg ggaagttgtt tgaccaatct
     3661 tcatataaag caaccatttc agcagcagtt aactcttttc cttctcctgc taaaacgtat
     3721 tttttagttt ctttgttgta catttctgta gcagcaacgt caagtcctaa cataacatct
     3781 tcacctggct tatatccagc ttttgttata gcttcaacta ttaattctaa agcttctctg
     3841 tttgaaccta agtttggagc aaatccacct tcgtcaccaa caccacaagc taatcctttt
     3901 tctcctaaaa ctttctttaa tgaatggaat acttctgctc ccattcttaa accttcttta
     3961 aagcaacaag ctcctactgg taatatcatg aactcttgaa catcaacgtt attatcagca
     4021 tgctctccac cgtttaatat gttcatcatt ggaacaggta attgtttagc atttactcca
     4081 cctaaatatt ggaataatgg taaacctatt tcatcagcag ctgctctagc tactgccatt
     4141 gaaacaccta atatagcatt agctcctaat ttacctttgt ttggagttcc atctaattct
     4201 atcatgattg catctatagc tggttggtca aatgcatcca ttccttctat ttcaggtgct
     4261 attatttcat ttacgttagc aacagctgtt tctacacctt ttcctaagta tcttccctta
     4321 tctccatctc ttaattcaac agcttcaaaa gctcctgtag atgctcctga tggaactatt
     4381 gctcttccca ttgctccatc ttctaatact acttcaactt ctacagttgg atttcctctt
     4441 gagtctaata cttctctagc atatactaat tcaataactg acatttgtat atctcccttc
     4501 gattttctat tagttaaatt ttcatttact gtaaacatcc gtctactatt ttaatctact
     4561 ttctatatta tagtacataa agttgctttt gaaaattagg tacaaattta tttgatgggt
     4621 aaatttgtaa aagttcctat ttttatttta ttttttataa tattttaatt tatataatat
//
LOCUS       DAMJWP010000250         4581 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.257, whole genome shotgun sequence.
ACCESSION   DAMJWP010000250 DAMJWP010000000
VERSION     DAMJWP010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.257"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     misc_binding    126..360
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            410..1429
                     /gene="pheS"
                     /locus_tag="PC700_003280"
     CDS             410..1429
                     /gene="pheS"
                     /locus_tag="PC700_003280"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDF2582549.1"
                     /translation="MQEKLLALREAALAEIKEAQSIESVESLRVKYLGKKGEITAILK
                     EMGKLSAEERPVVGKVANEVRENIELSINSKKEEINAIEKERKLKEEVIDVTQPGKVL
                     KVGKKHPITQIIDEVTDIFIGMGFSIAEGPEVETVENNFDALNAPKDHPSRDMSDTFY
                     INDGVLLRTQTSPVQVRTMRSQELPIKVIAPGRCFRSDSPDATHSPMFHQIEGLVVGK
                     DVTMAEFKGTMDIFVEKLFGSDIKTKFRPHNFPFTEPSAEVDVTCFKCGGKGCPMCKY
                     EGWIEILGSGMVHPNVLRNCGIDPEVYSGFAFGVGVERLAMLKYEIDDIRLLFENDMR
                     FLNQF"
     gene            1446..3839
                     /locus_tag="PC700_003281"
     CDS             1446..3839
                     /locus_tag="PC700_003281"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDF2582550.1"
                     /translation="MLVSLKWLRDYVDIDMDVKEFADKMTMTGTKVETIDYYGEEIEN
                     ILVGKILEIKQHPNADKLVVTKVDIGDKVVQIVTGATNISEGDYIPVAVNGSKLPGGV
                     EIKQTDFRGELSDGMMCSAAELGIDEHYIEEYKRGGIYILDHEDSYELGKDIKDVLGL
                     KDALIDFELTSNRPDCKCMMGIAREAAATIGTKVKYPEIEVKESDEEIDFKVEIDNPD
                     LCRRYVARMVTDVKIEPSPYWMQRRLTEAGVRPISNIVDITNFVMLELGQPLHAFDIN
                     QVETGRIVVRNAKDGEKLVTLDDVERTLDKDMLVITNGEKSLGLAGVMGGANSEITSN
                     TKTVLFESANFKPENIRMTAKKVGIRSEASSRNEKDLDPNLAEIAANRAAQLVEMLGA
                     GKVLKGVVDVYPNKPEPKKLVVNPQRINHLLGVDVPMEQFVGILESLEFKCNLVANDK
                     LEIDVPSFRTDMEQEADVWEEIARIYGFENIPSVQLEGNTTAGIKTSKQKFMDALKDN
                     STAVGLNEILTYSFVSPKGVDKIRVPEGNAKRNFVKLLNPLGEETSVMRTTLIPNMLD
                     VLSTNVSHKIEEVSAFECGHIFIPQDSELPKEENRMCVGMYGKDVDFFALKGTIETIL
                     VNVGFKGYEIEPQDNNTTFHPGRCAKIVYNNKYVGTLGELHPDVIENYNLGQRVYVAE
                     IDIDFVFDNSDRTKNYVPLPKYPSTSRDIALIVKDDVFVKQIEDIIKENGQGLVESYK
                     LFDVYKGSQIEAGYKSIAYSITYRSKDKTLTDEDVAKVHDKILSELSEKLNANLRSN"
     gene            3864..4454
                     /gene="zapA"
                     /locus_tag="PC700_003282"
     CDS             3864..4454
                     /gene="zapA"
                     /locus_tag="PC700_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDF2582551.1"
                     /translation="MNKVMVKIHGAEYPMVGDKSEKFMISIADFVDKEMDKITRQNPK
                     LSLSVAAILTALNISDLLFECSDENEKLIKANEELSKKVGASNEELQLEIKSLKLTIA
                     EKEAENREAETKMKELIEIIENKKQEIFELSNTTEGSRAELDVYKNKIEELSTQLEEA
                     NERATIAENLASEFQNKAYDLQLKCTGLNNDAKNVE"
BASE COUNT         1814 a          525 c          928 g         1314 t
ORIGIN      
        1 taattatgca ttagatatat attatataaa aaataattaa attaattatg tattggatat
       61 atattaaaaa tagtcacata atttgaatgg aaatgatata atactaaaat aaacaatata
      121 atattgtaaa tgcaatgaaa gaggaaagta ttttgattaa acttagtaaa gagataaaca
      181 cctaggctgg gagtgttttc taagaggtca tgagaagttc cctctggagt aacagagctg
      241 aaattttaca gtaggctttg acgtcaaaaa cgcgttaagt ttgttagagg tggtttgatg
      301 atttttaaat tgttaaacta ctagggtggt accgcgaaac tatagccttt cgtccctaga
      361 cagggattga ggggcttttt ttatacaaaa aaacgaaagg gtgatatgtg tgcaagaaaa
      421 attacttgct ttacgtgaag cagctttggc tgaaataaaa gaagcacaaa gcatagaaag
      481 tgtagaaagt ttaagagtta agtacttagg aaaaaaaggt gagataactg ccatacttaa
      541 agaaatgggt aaattatctg ctgaagaaag accagtagtt ggtaaggttg ccaatgaggt
      601 aagagaaaac attgaactta gcataaattc taaaaaagaa gaaataaatg ctattgaaaa
      661 agaaagaaaa ttaaaagagg aagtgataga tgttactcaa ccaggaaaag ttttaaaggt
      721 tgggaagaag catccaataa ctcaaattat agatgaagta acagatatat ttatcggaat
      781 gggattctct atagcagaag ggccagaagt tgagactgtt gaaaacaact ttgacgcatt
      841 aaacgctcct aaagaccatc catcaagaga tatgagcgat acattctata tcaatgatgg
      901 ggtattactt agaactcaaa catctccagt tcaagtaaga actatgagaa gtcaagagtt
      961 accaataaaa gtaattgcac caggtagatg ttttaggtca gactcgccag atgctacaca
     1021 ctcaccaatg ttccatcaga tagaagggct tgttgttgga aaagatgtta ctatggcaga
     1081 atttaaagga actatggata tcttcgttga aaaattgttt ggttctgata tcaaaactaa
     1141 gtttagacct cacaacttcc catttacaga accaagtgca gaggttgatg ttacttgttt
     1201 caaatgtggt ggtaaaggtt gcccaatgtg taaatatgaa ggttggatag aaatattagg
     1261 ttcaggtatg gttcatccaa atgtgcttag aaattgtgga atagacccag aagtttacag
     1321 tggatttgca tttggagttg gggttgaaag acttgcaatg cttaaatacg aaatagatga
     1381 tattagatta ttattcgaaa atgatatgag attcttaaat caattttaat taggagggta
     1441 tgtagatgtt agtatcttta aaatggctta gagactatgt tgatatagac atggatgtaa
     1501 aagagttcgc tgataaaatg acaatgacag gaactaaagt tgaaacaata gattattatg
     1561 gtgaagaaat agaaaatata ttggttggaa agattttaga aataaaacaa catccaaatg
     1621 ctgataagtt ggttgtaact aaagtagata ttggagataa agttgttcaa atagttacag
     1681 gagctacaaa tatatcagaa ggagattata ttccagtagc tgtaaatggt tctaagttac
     1741 ctggaggagt tgaaatcaaa cagactgatt tcagaggtga attatcagat ggtatgatgt
     1801 gttcagcagc tgaactaggt atagatgaac attacattga ggagtataaa agaggtggta
     1861 tatatatttt agaccacgaa gattcttatg aattaggaaa agatataaaa gatgttttag
     1921 gattaaaaga tgctttaata gattttgaat taacttcaaa cagacctgat tgtaaatgca
     1981 tgatgggtat agctagagaa gcagctgcaa ctataggaac aaaagtaaaa tatcctgaaa
     2041 tcgaagtaaa agaaagtgac gaagagatag atttcaaagt tgagatagat aatccagatt
     2101 tatgtagaag atatgttgct agaatggtta cagatgtaaa aatagaacct tctccatatt
     2161 ggatgcaaag aagacttaca gaagcaggag taagacctat aagtaacata gtcgatataa
     2221 caaacttcgt aatgttagag cttggtcaac cacttcatgc ttttgatata aatcaagtag
     2281 agactggaag aatagtagta agaaatgcta aagatggaga gaaacttgta acattagatg
     2341 atgttgagag aacattagat aaagatatgc tagttataac aaatggagaa aaatcacttg
     2401 gtttagctgg tgtaatgggt ggtgctaact cagaaataac ttctaatacg aagactgtac
     2461 tttttgaaag tgccaatttc aaaccagaaa acataagaat gacagctaaa aaagttggta
     2521 ttaggtcaga agcatcttca agaaatgaaa aagacttaga ccctaatctt gcagagatag
     2581 cagcaaatag agctgcacaa cttgttgaaa tgttaggagc aggaaaagtt ttaaaaggtg
     2641 ttgtagatgt atatccaaat aaaccagaac ctaaaaaatt ggtagtaaat cctcaaagaa
     2701 ttaaccacct attaggtgta gatgtaccaa tggagcagtt tgtaggaatt ttagaatcat
     2761 tagagtttaa atgtaatttg gtagctaatg ataaattaga aatagatgta ccaagcttta
     2821 gaacagatat ggaacaagaa gctgatgtat gggaagaaat agctagaatt tatggatttg
     2881 agaatatacc ttctgtacaa ttagaaggaa atacgacagc aggaattaaa acttcaaaac
     2941 aaaaattcat ggatgcttta aaagataatt caactgctgt aggattaaat gaaatattaa
     3001 catattcatt tgtaagccct aagggagtcg ataagataag agtaccagaa ggtaatgcta
     3061 agagaaactt tgttaaatta cttaacccat taggagaaga aacttctgta atgagaacta
     3121 ctttaatacc aaatatgtta gatgttttat caactaatgt atctcataaa atagaagaag
     3181 tatctgcatt tgagtgtgga catatattca tcccacaaga ttcagaatta cctaaagaag
     3241 aaaatagaat gtgtgtaggt atgtatggta aagatgttga cttctttgca ctaaaaggaa
     3301 ctatagaaac tatacttgta aatgtcggat ttaaaggtta tgaaattgaa cctcaagata
     3361 ataatactac cttccaccct ggtagatgtg ctaaaatagt gtataataat aaatatgtag
     3421 gtacattagg tgaattgcat ccagatgtta tagaaaacta caatctaggt caaagagttt
     3481 atgtcgctga aatagatatt gactttgtat ttgataattc tgatagaaca aagaattatg
     3541 tgccattacc aaaatatcca tctacatcaa gagatatagc tcttattgtt aaagatgatg
     3601 tatttgtaaa acaaatagaa gatataataa aggaaaatgg tcaaggatta gtagaaagct
     3661 acaagttatt tgatgtttac aaaggttctc aaatagaagc tggatacaag tcaattgcgt
     3721 attcaataac ttatagaagt aaagataaaa ctctaacaga tgaagatgta gctaaggtgc
     3781 atgataagat attaagtgaa ttaagcgaaa aattaaatgc taacttaaga tcaaattaac
     3841 aactaatgtc gggggaggtt aggatgaaca aagtaatggt taaaatccat ggcgctgaat
     3901 atccaatggt tggagacaaa tcagagaagt ttatgattag tattgctgat tttgtagaca
     3961 aagaaatgga taaaataaca cgtcaaaacc cgaaattgag cttatcagtt gctgcgatac
     4021 taactgcatt aaatatatca gaccttttat ttgaatgttc agatgagaat gagaagttga
     4081 ttaaagctaa tgaagaattg agtaagaagg ttggagcatc taatgaagag cttcaattag
     4141 aaatcaagtc tttaaagctt acgatagcag aaaaagaagc agaaaatcgt gaagctgaga
     4201 ctaagatgaa agagttaatt gagattatag aaaataaaaa gcaagaaata ttcgaactta
     4261 gtaatacgac agaaggttct agagcagagt tagatgttta taaaaacaag attgaggagt
     4321 taagtactca acttgaagaa gcaaatgaaa gagctactat agctgaaaac ttagcttctg
     4381 agtttcaaaa caaagcttat gatttacagt taaaatgtac agggttaaat aatgatgcaa
     4441 aaaatgtgga ataaactaaa agatttgctg tctcttgttt tttgagacag cttcttttaa
     4501 tgtataaata taaatgctat ataaatatag ctaatgttta aatgtaaata aatataaatg
     4561 ctatataaat atagataatg t
//
LOCUS       DAMJWP010000251         4533 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.258, whole genome shotgun sequence.
ACCESSION   DAMJWP010000251 DAMJWP010000000
VERSION     DAMJWP010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4533
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.258"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            202..1008
                     /locus_tag="PC700_003283"
     CDS             202..1008
                     /locus_tag="PC700_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2582552.1"
                     /translation="MGMILSTLGINLYLAPAKLLSGGVAGICVILYKLFGINQGISSF
                     LMNIPIFIIARKYFDNKFLLISFVNMLLFSLALGLTQDIAKYFPIDDTMLQCIYGGAL
                     TGIGMGLTFKARATAGGLDIIAAIMKRKYDIPMKNTFLFINFFVVCAGAFLFGAKLVM
                     YTLITMYIISFTMDIGKDCFDRKKSILLISNKYDEISKVIMNKMGRGVTFLEAEGAYT
                     QNKKKMIYCIISANEIGKFKDLIYDMDSEAFISVNNVEEVKGGGFKDKFL"
     gene            1135..1407
                     /locus_tag="PC700_003284"
     CDS             1135..1407
                     /locus_tag="PC700_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582553.1"
                     /translation="MLRERFVVRNHHNPHTSGCCGTGGEGCCGGDNHEHSHQHSHSHG
                     HEHEHVNGVGHDHNHDDGFSGTHEHSHEHTHTHGHEHSDGHTHEHK"
     gene            complement(1570..2142)
                     /locus_tag="PC700_003285"
     CDS             complement(1570..2142)
                     /locus_tag="PC700_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2582554.1"
                     /translation="MKTNNMKQAILSSATNLIAKNGVQNTSLADIAKDVNISKGTLYY
                     HYASKDDIIYDIADTHLEVITSAILNCVKNVKSKNSQIEMVNLILEKISTIESRGRVH
                     MYLICEAITSNNDLKERIRLKYIEWRTTLKSEIVESLEKYNSSENEQDAESFSFLLMS
                     IVDGLVVQSLLKTEKIPYENIASFLVNHWI"
     gene            2326..3168
                     /locus_tag="PC700_003286"
     CDS             2326..3168
                     /locus_tag="PC700_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDF2582555.1"
                     /translation="MDKIKLIVDSACDLPDDIIEKYNIEVVGLNVSFGEESYISGKEI
                     DNETFYRKMSESKVLPKTSCPSPDKFLEAYHCQEESVLVINISSGLSGTYNSALLAKD
                     MFEKEGNNKKVEVIDSLSGSIGIGQLVLKAAILIKEGKEIEEIVSIINKYKDNPPFFG
                     TLETLENAIKGGRINPIAGKIINTLNFKAIIQVADGVVTPIDKARGEGNSLKKLITLV
                     ESKIKDKEEKVLFIGHANCPEKAQKVREVMEKDVKYKDVVICEIGSVMGTYTSKGAIL
                     ITAI"
     gene            complement(3271..4347)
                     /locus_tag="PC700_003287"
     CDS             complement(3271..4347)
                     /locus_tag="PC700_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2582556.1"
                     /translation="MKAQRLNAVLEQMKKDDISQMLVSDPTSIFYLTGVLIHPGERLL
                     ALYLNLNGNNKLFINELFPVSEDLGVEMVWFNDTQNPVEIITEHIDKNATMGVDKNWP
                     ARFLLNLIELGGGSKFVNSSYIIDTLRMCKDEEEKELMRIASKLNDKAMEQLKATVSG
                     ELTEKQLVGKLSKIYEDLGTDGFSFDPIIGFGPNGANPHGEPGNALVKPGDAIILDIG
                     CIKDNYCADMTRTVFYKEIPEKGREIFEIVLEANKRAEAIVKPGVRFCDIDAAARDYI
                     TEKGYGQYFTHRTGHSIGLEVHDKGDVSSINTDTVQPGMIFSIEPGIYLPGEFGVRIE
                     DLVLVTEDGCEILNKHDKEICVVG"
BASE COUNT         1651 a          586 c          709 g         1587 t
ORIGIN      
        1 ataaatatat taaaagaagg attaagacta ataacaattc aaaataaata acttatgtaa
       61 gaaccgtagg aactacgggg atagcttgtg gagaattagt aagacatatt ttagtatgca
      121 aaattctatg aagcaggaac cctgtgactt tagtcatggg aggttcagaa taacatattt
      181 ttacaatatg gtctgattat attgggtatg attctatcaa ccttaggaat aaatttatat
      241 ctagcaccag ccaagttatt aagtggagga gtagctggaa tttgtgttat attatacaaa
      301 ttatttggta taaatcaggg aatatcaagt ttcttgatga acataccaat atttattata
      361 gctagaaagt attttgataa taaatttttg ctaataagtt ttgtcaatat gttactgttt
      421 tctctagcac tggggctaac acaagatatt gctaaatatt tcccaataga tgatactatg
      481 ctacagtgta tttatggtgg tgcattaact ggtataggta tggggttaac ttttaaagca
      541 agagctactg ctggaggact tgatataata gcagccataa tgaaaagaaa gtatgatata
      601 cctatgaaaa atacattttt atttattaat ttttttgtag tatgtgcagg agctttctta
      661 tttggagcta aattggtgat gtacacttta attacaatgt atataatttc ttttactatg
      721 gatataggaa aagattgctt tgatagaaaa aaatctatac ttttaatatc aaataaatat
      781 gatgaaatat ccaaagtgat aatgaataaa atgggtagag gagtcacttt tttggaggca
      841 gaaggtgcat atacacaaaa taagaaaaag atgatttatt gcataatatc tgcaaatgaa
      901 ataggtaaat ttaaagattt aatttatgat atggatagtg aagcttttat ttctgtcaat
      961 aacgttgaag aagtaaaagg tggaggtttt aaggataaat ttctttaaga ttatatatgt
     1021 taattatata tgttaaaaaa tgccatatgt attgagatta tacctagtta gatgtatact
     1081 gtagttaatg gggtacatga agtattcaat aatatactag gaggtataag aattatgtta
     1141 agagagagat ttgtagtaag aaatcatcat aatccacata caagtgggtg ttgtgggact
     1201 ggaggagaag gatgctgtgg aggagataac catgaacatt cacatcaaca ttctcacagt
     1261 catgggcatg aacatgagca tgtaaatggt gtaggtcacg accataatca tgatgatggt
     1321 ttttcaggta ctcatgagca tagccatgag catacacata cacatggtca tgaacattca
     1381 gatgggcata cacatgaaca taaataaaaa cattttaaat ttataagaga tttttatttt
     1441 gtgattccat gaaggtttaa ataaaaattg aatgttttaa gtaaaaatta ttgagaataa
     1501 ataagaaagt gaactgttaa agtagataac ttaaatagtt cacttttatt aattatctaa
     1561 gtattaagtt taaatccaat gattaactaa aaaagaagct atattttcat aaggtatttt
     1621 ttctgttttt aataaacttt ggacaactaa accatcaact attgacatga gtaaaaaaga
     1681 aaatgattca gcatcctgtt cattttcgct actattatat ttttctagtg attcaacaat
     1741 ttcagattta agagtagttc tccattcaat atattttaat cttattcttt cctttaaatc
     1801 attatttgaa gtaattgcct cacaaataag atacatatgt accctaccac gactttctat
     1861 agtagatatt ttctctaaga ttaagttaac catttctatt tgagaatttt ttgatttgac
     1921 attttttaca cagtttagta tagcactagt tataacttct aaatgagtat ccgcaatatc
     1981 atatataata tcatctttag atgcgtagtg ataatacaaa gtacctttac ttatatttac
     2041 atcttttgca atatcagcaa ggctagtatt ttgaactcca tttttggcta ttaaattggt
     2101 tgcagatgat aaaatagcct gtttcatatt atttgttttc ataaaaccta cctttccatg
     2161 tatatttata acaaattaat aacattataa gcttatgaat atttattgtc aataaagcga
     2221 gaatctatat ctattgactt tttttcattc aatatataaa ataataatta aatcggtcgt
     2281 acgaccgact gaataaataa aataatatct tatagtgagg ttaatatgga taaaataaaa
     2341 ttgatagtgg acagtgcatg tgacttacct gatgatataa tagaaaaata caacatagaa
     2401 gttgttggtt taaatgtatc ttttggagaa gaaagttata tatctggaaa ggaaatagat
     2461 aatgaaactt tctatagaaa aatgagtgag agtaaagtac taccaaaaac atcatgtcct
     2521 tctcctgata aatttttaga agcataccat tgtcaggaag aaagtgtatt ggtgattaat
     2581 ataagttcag gattatctgg gacttataat agtgcacttt tagcaaaaga tatgtttgaa
     2641 aaggaaggaa ataacaaaaa agttgaggtt atagattcac ttagtgggtc aattggaata
     2701 ggacaattag tgttaaaagc agcaatatta ataaaagaag gaaaagaaat tgaagagatt
     2761 gtttcaataa taaataaata taaagataat cctccatttt ttggaacact tgaaacttta
     2821 gaaaatgcta ttaagggtgg tagaataaat ccaatagcag gaaaaattat aaataccttg
     2881 aattttaagg cgataataca agttgcagat ggcgttgtaa ctcctataga taaggcaaga
     2941 ggtgaaggaa acagtttgaa aaagctgatt actttagtag agtcaaaaat taaagataaa
     3001 gaagaaaagg ttttatttat aggacatgct aattgcccag aaaaggctca aaaggtaagg
     3061 gaagttatgg aaaaggatgt aaaatacaaa gatgttgtaa tctgtgaaat tggttcagtg
     3121 atggggacat atacttcaaa gggtgcaatc ttaataactg caatataaat aatggattga
     3181 aataaatcat agattgagaa attaaagcca tataaaaatg tttaaaacat ttttatatgg
     3241 ctttttaatt ttatcaaatt gattttagaa ttaaccaact acacatattt ctttgtcatg
     3301 tttgtttaat atctcacaac catcttcagt aacaagtact aaatcttcta ttctaactcc
     3361 gaactcacct ggtaaatata tacctggctc tatagagaat atcattcctg gttgtacagt
     3421 atctgtattt atagaagata catctccttt atcatgaact tcaagtccta tagagtgacc
     3481 agttctatga gtgaagtact gtccatatcc cttttcagtt atgtagtcac gagcagcagc
     3541 atctatatca cagaatctaa cacctggttt tacaatagct tcagctctct tattagcttc
     3601 aagaactatt tcaaatattt ctctaccttt ttcaggtatt tctttataga atacagttct
     3661 tgtcatatca gcacaatagt tatcttttat acaacctata tctaatatta ttgcatctcc
     3721 tggtttcaca agtgcatttc caggttcacc atgtggatta gctccattag gtccaaaacc
     3781 tataataggg tcaaatgaaa atccatcagt acctaggtct tcatatattt tagataattt
     3841 accaacaagt tgtttctctg taagttcacc agaaacagta gctttaagtt gttccatagc
     3901 cttgtcattt aattttgaag ctattctcat taactctttt tcttcttcat ctttacacat
     3961 tcttaaagta tctattatat aagatgaatt tacaaactta cttcctccac ctaattctat
     4021 taagtttaat aagaatctag ctggccagtt tttatcaaca cccatagtag catttttgtc
     4081 tatatgttca gttattattt ctactggatt ttgagtatca ttaaaccaaa ccatttcaac
     4141 acctaaatct tctgaaactg ggaataattc atttataaat aatttattat tgccatttaa
     4201 atttaagtat aatgctaata atctttcacc tggatggatt agtactcctg taagataaaa
     4261 tatagaagta gggtcactta ctaacatttg agaaatatca tcttttttca tttgttcaag
     4321 tacagcattt aatctttgag ctttcataat aatttcctcc taatcaaaat tcaatacaaa
     4381 tcaattttag cattattaaa caaaaaagag tatacttttc ttctagaaat ataaaaaaag
     4441 tttgttatat attttttata ttaagtttaa tctctacttt tgcattaaat acggaagtaa
     4501 aatgactttt tattgtgaaa attctataaa agt
//
LOCUS       DAMJWP010000252         4505 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.259, whole genome shotgun sequence.
ACCESSION   DAMJWP010000252 DAMJWP010000000
VERSION     DAMJWP010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.259"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            203..1678
                     /locus_tag="PC700_003288"
     CDS             203..1678
                     /locus_tag="PC700_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDF2582557.1"
                     /translation="MDSEFANAVELSYECAPLLESLKEYSEKDIACFDVPGHVKNRGV
                     AILNKYLGESLMKMDINSSPTMDNVSAPNGIIKNAQDLLAQAYMADEAFFITNGTTQA
                     IHAMILSVIKPGEKVLLPRNIHKSVINALILCGGIPIFIQPEFDEKLGISLNITLEKV
                     RTEIEKDCNIKALFFLNPTYYGVCADLESIIELCHKNNVLVLVDEAHGAHFPFHLDLP
                     PSAISLGADMVAVSIHKTGGALTQSSALLLNRDNVSFEKVLQSINMLQSTSASYLLMA
                     SIDGARVNLVENGEKQLSKALNLSRYAKSKLNKIDGIKVLSTEILKQKGVKFIDETKL
                     CINVKELNLTGFEVYDLLYKNFSIQVELGDSYNILALVSIGTNKSDIDRLVKALSIIA
                     KIYRKESTLNEFNMVQINPIIKLNPREAFYAPKESVEINSCIDRICGESIMAYPPGIP
                     IIAPGELITEEIMEYIIFLKNSNAYLTDVQDKNLDRILVIK"
     gene            1765..2178
                     /gene="speD"
                     /locus_tag="PC700_003289"
     CDS             1765..2178
                     /gene="speD"
                     /locus_tag="PC700_003289"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HDF2582558.1"
                     /translation="MKFETLGRHILVEYYNCDENVLKDPRLIENFMNDSALNAKATIV
                     DSVFHHFNPWGVSGAVIIAESHLTIHTWPEYGYAAADFFTCGDIDPWKSFELLEQLLK
                     SERSESTEIPRGLTTKIKKYSKKDLGKITHKPEAV"
     gene            2208..3059
                     /gene="speE"
                     /locus_tag="PC700_003290"
     CDS             2208..3059
                     /gene="speE"
                     /locus_tag="PC700_003290"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HDF2582559.1"
                     /translation="MELWYTEEWTENVRFSIKVNKHLFEGKSQFQRIDVFDSDEFGKF
                     LTIDGLMMVTYKDEFIYHEMITHVPMATNLNIKKVLVIGGGDGGTVRELSRYPQIEKI
                     DMVEIDKMVVDVSKEYMDICSCKLDDKRVSLYFEDGVNFVKCAHDKSYDLIIVDSTDP
                     IGPGEGLFSTDFYKDCYRILTDDGILVNQSESPYFDFNAKEMKRANKKLKQIFPISEV
                     YQAHIPTYPSGHWLFGFASKKLNPVKNQDRNGWEKLSLKTKYYNSDIHLGSFMLPQYV
                     KEMLDEE"
     gene            3049..3927
                     /gene="speB"
                     /locus_tag="PC700_003291"
     CDS             3049..3927
                     /gene="speB"
                     /locus_tag="PC700_003291"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HDF2582560.1"
                     /translation="MKNNFYHMNTFMSMDKNYEESNLIVFGVGFDGTTSNRPGARFAS
                     SSMRKEFYGLETYSPFLDLDLEDYNICDYGDLEISVGSTEQVLKEIYQETYKIVRDSK
                     VPFMIGGEHLVTLPAFKAVHEKYNDIYVIHFDAHTDLREEYNNSKNSHATVIKRIWDI
                     VGDNKIFQFGIRSGTKEEFKFATEEKHTYMEIGGIDTFENIVNMLNGKNIYLTIDLDV
                     LDASVFPGTGTPEPGGVNYREFQEIFKIIKNSNINIVGCDIVELSPDYDTTGVSTVIA
                     CKILRELCLIISDKIK"
     gene            complement(4107..4307)
                     /locus_tag="PC700_003292"
     CDS             complement(4107..4307)
                     /locus_tag="PC700_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDF2582561.1"
                     /translation="MKNGIVKWFNNEKGFGFISVEGEDDVFVHFSAIQNDGYKTLEEG
                     EKVSFDITQGNRGPQAENVNRI"
BASE COUNT         1788 a          502 c          768 g         1447 t
ORIGIN      
        1 caacaaaaaa ataaaaagtt tataaatatt gatatagcta atgtatatgt ttttgagtaa
       61 cttcaaaaaa acacttgtaa aaatgctgga atgttaatat aataaatatc atgagtttaa
      121 taaaagtaaa aataaaaaat agaaataata aataataaat attaaaaata tttgaatata
      181 gagaggagat gaatttgaaa tcatggatag cgagtttgca aatgcagttg aattaagcta
      241 tgagtgtgct ccattattag agtcgttaaa ggaatactca gagaaagata tagcttgttt
      301 tgatgttcct ggacatgtaa aaaatagagg tgtagctata ttaaataaat atctaggtga
      361 aagtctcatg aaaatggata taaattcatc accaactatg gataatgtat ctgcgccaaa
      421 tgggattata aaaaatgcac aagatttgct agcacaagca tatatggctg atgaggcatt
      481 ctttataacg aatggcacaa ctcaagcaat acatgcaatg attttgagtg ttataaaacc
      541 aggtgaaaaa gtattacttc caagaaatat acataaatca gtaataaatg cgttaatact
      601 ttgtggagga ataccaatat ttatacaacc agaatttgat gaaaaactag gtataagttt
      661 aaatattact cttgaaaaag ttagaacaga gatagaaaaa gattgtaata taaaagcatt
      721 atttttcctt aatccaactt actatggagt ttgtgcagac cttgagagta ttatagaact
      781 atgccataaa aataatgttt tagtgttagt tgatgaagct catggtgcac attttccatt
      841 tcatttagat ttacctcctt ctgcaataag tttaggagct gacatggtag ctgtaagtat
      901 acataaaact ggaggagcat tgacacaatc ttcagcactc ttattgaata gagataatgt
      961 aagttttgaa aaggttcttc aatcaatcaa tatgctacaa tcaacatcag catcttattt
     1021 gcttatggca agtatagatg gtgctagagt taatctagtt gagaatggtg aaaagcaact
     1081 gtcaaaagca ttaaatctat ctaggtatgc aaaatctaaa ttgaacaaaa ttgatggaat
     1141 aaaagtttta tctacagaaa tattgaaaca aaaaggtgtt aaatttatag atgaaacaaa
     1201 attgtgtata aatgtaaaag aattaaattt gactggattt gaagtatacg atttattata
     1261 taaaaatttt tcaattcaag tagaattggg agattcatat aatatactgg cacttgtgtc
     1321 cattggaaca aataaatcag atatagatag attagtaaaa gcactttcta taattgcaaa
     1381 gatttataga aaagaatcaa cattaaatga atttaatatg gttcaaataa atccaattat
     1441 taaacttaat ccaagagaag ctttttatgc accaaaggaa agtgtagaaa tcaattcatg
     1501 tatagataga atttgtggag aatcaataat ggcatatcca ccaggtatac cgattattgc
     1561 tccaggagaa ttgataacag aagaaattat ggagtatatt attttcttaa aaaatagcaa
     1621 tgcgtatctt acagatgtac aagataaaaa cttagataga atactagtaa taaaataaat
     1681 tactttattt tgcaagggca agtgtttgtt aagtagagtt tatcaaatag aacttattaa
     1741 aagtataaag gagttggggt tgaaatgaaa tttgaaacac taggaagaca tatactagta
     1801 gaatactata attgtgatga aaacgtactt aaagacccac gtcttataga aaattttatg
     1861 aatgattcag cattaaatgc aaaagcgaca attgtggatt ctgtattcca ccattttaat
     1921 ccttgggggg tttcaggagc tgttataatt gctgaatctc atttgacaat acatacatgg
     1981 cctgaatatg gttatgcagc agcagatttt tttacttgtg gtgatataga cccatggaaa
     2041 agttttgaat tattagagca acttttaaag tctgaaagaa gcgagtctac agaaattcca
     2101 agaggcttaa ctacaaaaat taaaaaatat tcaaaaaaag atttaggtaa gattactcat
     2161 aaaccagaag cagtataaaa catacaagag aaagtgagga ttattatatg gaactttggt
     2221 atacagaaga atggacagaa aatgtaagat tttctataaa agtaaataaa catctatttg
     2281 aaggaaagtc tcaatttcaa aggatagatg tatttgatag tgatgaattt ggaaaatttc
     2341 taacaataga tggattaatg atggtaacat ataaagatga atttatatac catgagatga
     2401 taactcatgt tcctatggct acaaatttaa acataaagaa agtattagtc ataggtggag
     2461 gtgatggagg tacagtaaga gaattaagcc gttatcctca aatagaaaag attgacatgg
     2521 ttgaaataga taaaatggtt gtagatgtat ctaaggagta tatggatata tgctcatgta
     2581 aattagatga taaaagagtg agtttatatt ttgaagatgg tgttaatttt gttaagtgtg
     2641 cacatgataa atcatatgac ctgataatag tagattcaac tgacccaata ggaccaggcg
     2701 aagggctttt ctcaactgat ttttataagg attgttatag aatactgact gatgatggaa
     2761 ttttagtaaa tcaaagtgaa agtccgtatt ttgactttaa tgctaaagag atgaaaagag
     2821 ctaataaaaa attaaaacag atatttccaa tatcagaagt ttatcaagca catataccaa
     2881 catatccatc tggtcactgg ttgtttggat ttgcttcaaa aaaacttaat ccagtaaaaa
     2941 atcaagatag aaatggatgg gagaaattaa gtttaaaaac taaatattat aatagtgata
     3001 tacatttagg ttcgtttatg ttacctcaat acgtgaagga gatgctagat gaagaataat
     3061 ttctatcata tgaacacttt tatgagtatg gacaaaaatt atgaagaatc taatcttata
     3121 gtatttggtg ttggatttga tggaactact tctaacagac ctggggctag atttgcaagt
     3181 agctctatga gaaaagaatt ttacggtctt gagacatata gtcctttttt agatttggac
     3241 ttagaggatt ataatatatg tgattatgga gatttagaaa ttagtgttgg aagtacagaa
     3301 caagtcttaa aagaaatcta tcaagagaca tataagattg ttagagattc aaaggtaccc
     3361 tttatgattg gaggagagca tttagttaca ttaccagcct ttaaagcagt acatgaaaag
     3421 tacaatgata tatatgtaat tcattttgat gcccatactg atttgaggga agaatataat
     3481 aatagtaaaa attctcatgc aacagtaatt aaaagaatat gggatattgt aggtgataat
     3541 aaaatatttc aatttggtat aaggtctggg acaaaagaag aatttaaatt tgctacagaa
     3601 gaaaaacaca catacatgga aataggagga atagatacgt ttgaaaatat agttaatatg
     3661 ctaaatggaa agaatattta tctaactata gatttagatg tattggatgc atctgtgttt
     3721 ccgggaacag gtacaccaga acctggtggt gtaaattata gagagtttca agagattttt
     3781 aagattataa aaaactctaa tataaatata gttggttgtg atattgtaga gttaagccct
     3841 gattacgata caacaggtgt atcgacagtt atagcttgta aaatcttaag agagttatgc
     3901 ttaataatat ctgataaaat taaatagagt attttatata aatagtatat attataatat
     3961 aagaagttat tttaaaaaag ttagtttaag tttgacgctt ttctaaagta acttcttatt
     4021 tttgtaatta aaaataaata taataaacaa caaaaaagag ccctcgatag ctccttttct
     4081 aaaatatata tatttaaatt aaataattat attctattta cgttttcagc ttgaggtcct
     4141 ctattacctt gagttatatc aaagctaacc ttttcgcctt cttctaaagt tttgtatcca
     4201 tcattttgta tagctgagaa atgtacgaaa acatcatctt ctccctctac agatatgaac
     4261 ccaaatcctt tttcattgtt aaaccatttt actattccgt ttttcattat aagaaaacct
     4321 ccgcattatt aaaaaataca aactaaaaaa ataagtctgt tctattaata taacataaat
     4381 tttaaataaa agcaaataaa atttttaaaa aaattaatta cttttagaat ttaataaaaa
     4441 ttgttaaatt atacagtgag aagtattgta aaatttgacg aaaatgtaaa tgtagataat
     4501 aaatt
//
LOCUS       DAMJWP010000253         4451 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.260, whole genome shotgun sequence.
ACCESSION   DAMJWP010000253 DAMJWP010000000
VERSION     DAMJWP010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4451
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.260"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(73..618)
                     /locus_tag="PC700_003293"
     CDS             complement(73..618)
                     /locus_tag="PC700_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HDF2582562.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEKNWADE
                     HRIGVAQGVDEEIIEGLRANFVGECTEVGMYLAMSRQADREGYPEVGEAYKRIALEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKKAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRYFGK"
     gene            complement(736..2559)
                     /locus_tag="PC700_003294"
     CDS             complement(736..2559)
                     /locus_tag="PC700_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2582563.1"
                     /translation="MNLSTGNQKVSRLKKKEKPKDFKKTFKRIFMYFKKDKKITRFIF
                     LLVIIDSIISTVIPYTIGKVVDIIDIDTYSKSIFLKGIIILLSFYIIEFSVKLSKGIC
                     TARLSQSIVKEMRSNLFLKFKNLPITFFDKSSTGDIMSRVTNDVDNISTGISNSLVQL
                     ITGILNILMTFIMMFVLSPILTFCSVILIPIVMFVSNFIAKRTRVFFKQQQVELGKLN
                     AHIEEMISNINVVKSFNYERKSVNDFKEVNDRLLNISLKSQIYTSLLMPIMNVISNIG
                     FATICIIGGVLASKDIITIGVIASFLSYVRQFTRPLNELANLFNTLLSAIAGCERVFE
                     IIDEQEEQEVHNMFKSEINDNKDGNLNSHFVKGEIELKDVSFSYDSKKKILDNINLKI
                     KAGTSNAIIGETGSGKTTIINLITNFYHIDEGSIFLDGKDIYSINRNYLRTCFGVVPQ
                     EGYIFNDTVMENIRYGNLEATDEEVIYASKIANAHKFIVKMREGYSTVLSDRGEDLSE
                     GQKQLISIARAVLKNAPILILDEATSNIDIATEAKIQEAINNLTYGKTSIIIAHRLST
                     ISNCDNIIVLENGRIVESGNHKELIKLKGHYYRNILLTQGK"
     gene            complement(2572..4347)
                     /locus_tag="PC700_003295"
     CDS             complement(2572..4347)
                     /locus_tag="PC700_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2582564.1"
                     /translation="MKYFKKYIKKYIVLFLTAVFFLTLEAFCDLAQPTIMAKIIDIGV
                     SNKNLDYILSTGAIMIGITFLGAIFAIIRSVISARVSQHFSYDLRQDVFEKINTYSLK
                     QIDKFGRASLITRITNDINQMQQFVNGMMRVFIKSPIMCIGSLIIAIKLNLKMSIIIL
                     IAVLIIFIIILLNMKIGYRLFKNVQESTDKVNSKLRQFLSGIKVVKLFCRYDYENQSF
                     EELNDELFKNSRKATTMMSFFRPTITIIINVSIIIILLIGRHLIYSGEIKIGIIVAYV
                     NYMTRILTSLIMISHVFNVFVRAKASYERISEVLVDENEEVIEREESLDVGEKDLKFV
                     NCDIIFENVDFSYNKNTDEKILKNVSFKIEEGQNIGVIGQTGSGKTSLVNLIQKLYII
                     DKGDIKIGGRSIYSISEESLREKIGVVFQNSSVFGKSILENIVMGNEGISREDVIKSC
                     EIANANQFITKLENSYDEVLFQRGKNLSGGQRQRITIARAIAKKPQILILDDCFSAMD
                     INTETIIRKNIKEYISNLREKESKKCTVITISQKISSIIDCDKILVLDEGEVVGFATH
                     EDLLVSSPIYKKIFALQNLVSEDVN"
BASE COUNT         1446 a          794 c          394 g         1817 t
ORIGIN      
        1 aggagcatat cattataatt ttaaaaatca taaatgatat actcctaaaa atctacttaa
       61 cttatattaa atttattttc caaaatatct atttaataat cctaagaatg ctttaccatg
      121 tctagcttca tctttacaca tttcatgtac tgtatcgtgt atagcatcta gtcctaattc
      181 tttagctttt ttagctaatt ttaattttcc atcagttgca ccatactcag catcaactct
      241 aactcttaag ttttcttttg tatctgcaac tacaacttct ccaagaagtt ctgcaaattt
      301 agcagcatgt tcagcttctt caagagctat tctcttataa gcttctccta cttctggata
      361 accttctcta tcagcttgtc tactcattgc taaatacatt cctacttctg tacactcacc
      421 aacaaagtta gctcttaatc cttcgattat ttcttcatct acaccttgag ctactcctat
      481 tctgtgctca tcagcccaat ttttttcgcc tttcatttct tcaaatttat cagctccaac
      541 tttacatact ggacattgtg ctggtgcagc atctccttca tgtatatatc cacatactgt
      601 acaaacaaat ttcttcataa ttactttcct cctaaatttt atatatcaaa ttaaattttt
      661 tatttattaa gttagtctat atatacccgc accatatacc tttaaacaat ttttttaaaa
      721 gttttttcta aatacttatt ttccctgtgt caataatatg tttctataat aatgtccctt
      781 taattttatc aattctttat gatttcctga ttccacaatt ctaccatttt ctaaaactat
      841 tatattatca caatttgata tggtacttaa tctatgagct ataattatac ttgtctttcc
      901 ataagttaaa ttatttattg cttcttgaat tttagcttct gttgctatat ctatgttact
      961 tgtagcctca tccaaaataa gtattggagc attttttaat actgctctag caatacttat
     1021 taattgtttt tgaccttcac ttaaatcttc acccctatcc gataatacag tactatatcc
     1081 ttcacgcatt ttaactataa atttatgtgc atttgctatt ttactagcat atataacttc
     1141 ttcatcagta gcctccagat ttccatacct tatattttcc ataactgtat cattgaatat
     1201 ataaccttct tgtggaacta ctccaaaaca agttctcaaa tagtttctgt taatagagta
     1261 tatatcctta ccatctaaaa atatacttcc ttcatctata tgataaaaat ttgtaattag
     1321 atttataatc gttgtttttc cacttcctgt ctctcctatg atggcatttg atgtgccagc
     1381 tttgattttt aggtttatat tatctaaaat tttctttttt gaatcataag aaaaacttac
     1441 atctttaagc tcaatttctc cctttacaaa atgagagttt aaattaccat ctttattatc
     1501 gttaatctca cttttaaaca tgttatgcac ttcttgctct tcttgttcat ctataatttc
     1561 aaaaactctt tcacatccag ctatggctga aagtaatgta ttaaatagat ttgctaattc
     1621 atttaaaggt cttgtgaatt gtcttacata acttaaaaaa ctagctataa cccctatggt
     1681 tataatatct ttagatgcaa gaacacctcc tataatacat atagttgcaa atcctatatt
     1741 actaatcaca ttcataatag gcattaaaag agaagtgtaa atttgcgatt taagggaaat
     1801 atttagaagt ctatcattga cttctttaaa atcatttaca gattttcttt cataattaaa
     1861 agattttact acatttatgt ttgatatcat ttcttcaata tgtgcattta gttttcccaa
     1921 ctcaacttgt tgttgcttaa aaaaaactct agttctcttt gcaataaaat tactcacaaa
     1981 cattactatc ggtattaata ttacactgca aaatgttaaa attgggctta atacaaacat
     2041 cataataaaa gtcatcaata tgtttaagat tcctgtaatt aactgtacta atgaatttga
     2101 tattcctgta ctgatattat caacatcatt tgtcactcta ctcataatat ctccagtaga
     2161 acttttatca aaaaaggtta ttggtagatt tttaaatttt aaaaataaat tacttctcat
     2221 ttctttaact atactttgag ataatctagc tgtacatatc cccttagaca gtttaactga
     2281 aaactcaatt atataaaatg aaagtaaaat aattattcct tttaagaaaa tggattttga
     2341 ataagtatcg atatctatta tgtcaactac ctttccaata gtatatggta ttacagttga
     2401 tataattgaa tcaattatta ctagcaaaaa aataaatcta gtaatcttct tatctttttt
     2461 aaagtacata aaaattcttt taaacgtttt tttaaaatct ttaggctttt cttttttctt
     2521 taatctactg accttttgat ttccagtact taagttcaat atacttcctc cttaatttac
     2581 atcttcactt actaaattct gcaacgcaaa aattttttta taaattggag aactaaccag
     2641 taaatcttca tgtgtagcaa atccaaccac ctcaccctca tccaagacta aaatcttatc
     2701 acagtcaatt atagaactaa tcttttgtga aatagttatt acagtacatt tcttactttc
     2761 cttttctctt aaattactta tatactcttt tatattcttt cttataattg tctctgtatt
     2821 aatatccatt gcactaaaac aatcatcaag aattaatatt tgtggttttt tggctatagc
     2881 tctagctata gtaattcttt gtctctgtcc tcctgataaa tttttacccc tttgaaataa
     2941 aacctcatca taagaatttt cgagtttagt tataaattga tttgcatttg ctatctcaca
     3001 tgattttata acatcttctc tacttatacc ctcatttccc ataactatat tttctagtat
     3061 actttttcca aacacactgc tattttgaaa tactacacct attttttccc ttaaactttc
     3121 ttcagaaata ctgtatatac tacgtcctcc aatcttaata tcacctttat caattatata
     3181 taatttttga attaaattta ctaaagaagt ctttcctgaa ccagtttgac caatcacacc
     3241 aatattttga ccttcttcta ttttaaagga tacgtttttt aatatttttt catctgtatt
     3301 tttgttatac gaaaaatcta cattttcaaa aattatatca caatttacaa actttaaatc
     3361 tttttctcca acatctaaac tttcttctct ctcaattact tcttcattct catcaaccaa
     3421 aacctcactt attctctcat aagatgcttt tgccctaaca aaaacattaa aaacatgtga
     3481 aatcattata agagatgtta atattctagt catatagttt acataagcaa ctataatccc
     3541 aattttaatt tctcctgaat aaattaaatg ccttccaatc agtaatatta ttattatgga
     3601 aacattaatg attattgtaa ttgtcggtct aaaaaatgac atcatagttg tagcttttct
     3661 actattttta aaaagttcat catttaactc ttcaaagctt tggttctcat aatcgtatct
     3721 acaaaatagt ttaactactt ttattccact taaaaactgt cttaattttg aatttacttt
     3781 atctgtactt tcttgtacat ttttaaataa tctataccct atcttcatat taagcaatat
     3841 tataataaat ataattaaaa ctgcaattag aataattatt gacatcttta aatttaattt
     3901 tatagctata attaaacttc ctatacacat tattggtgac tttataaaaa ctctcatcat
     3961 tccattaacg aattgttgca tttgattgat atcattagta attcttgtta ttaacgaagc
     4021 tctaccaaat ttatctattt gttttaatga gtatgtattt atcttttcaa atacatcctg
     4081 tcttaaatca taagaaaaat gctgagaaac tcttgccgag attacacttc ttattattgc
     4141 aaatattgca cctaaaaaag ttatacctat cattatagct cctgttgata gtatgtagtc
     4201 taggttctta tttgaaaccc ctatatctat tatttttgcc attatagttg gttgagctaa
     4261 atcacaaaaa gcttccaatg ttaaaaaaaa tactgctgtt agaaataata ctatatattt
     4321 ttttatgtat tttttaaaat acttcattca atcatccctt catagaattt tctcattacc
     4381 aaaagtatat cataatggct aatttaattg attatatcaa ttataaatat ttgatttatt
     4441 attaattttt a
//
LOCUS       DAMJWP010000254         4299 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.261, whole genome shotgun sequence.
ACCESSION   DAMJWP010000254 DAMJWP010000000
VERSION     DAMJWP010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.261"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(732..1142)
                     /locus_tag="PC700_003296"
     CDS             complement(732..1142)
                     /locus_tag="PC700_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582565.1"
                     /translation="MKIKKSLISAMMVSAMVLGGTGAVFANGNDSSNIALPKLESLIK
                     SGITDPTELEKYGVEHYTYEEYAKHIQELKDYAKDPNAVKDVSQKDLEETIKKMEQEL
                     EKIKTEGLKIMKPITIENEDGSQISIAYNYAAID"
     gene            complement(1358..2740)
                     /locus_tag="PC700_003297"
     CDS             complement(1358..2740)
                     /locus_tag="PC700_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HDF2582566.1"
                     /translation="MFIKLLEMSFTSGVLLLIVIFIRACLFKKLPKKVFVLLWLIVLF
                     KLLIPVSLNSGFSIYSIIESTFDVELGNTSTENYTLKKASDSTGKNNSDEFEIMSNIE
                     NIMMLEMLGILSFIVTIMFSILLCFRYFKIINLFKTSIHIKNHEFIKKWMCKNSRFRR
                     FIVCISEFTRTPLTSGILKPRIILPKQMNYANNQTLDFVLTHEMVHINSFDNLLKLFM
                     LVALVVHWFNPLVWVMFFLLNKDIEFSCDEKVMSILGEEYRADYATTLISLAEENNPF
                     SMTICSGFGKTKIEKRIVNIMKFKKSTYVTFLVSTVLVCGTGLVFATENKEINEVESF
                     GLTKNEQSDKSGFSISSKSTDLYSPKVESDILVSKAKSDGETHFSTAKVSVIEFREHE
                     ESSMSKVTNSENSEETYVNKSSNSKDVLYTDSGEVSIEQSNYNDNRFVISRKGNLLAV
                     NKEQYRTWIE"
     gene            complement(2751..3110)
                     /locus_tag="PC700_003298"
     CDS             complement(2751..3110)
                     /locus_tag="PC700_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2582567.1"
                     /translation="MEIKLFDSELKVMEVLWERGESKAGELVKILKEETGWNRNTTYT
                     VIKKLVSKGAIERIEPNFVCKALISKEEVQKQETETLINKMFGGSSEKFLSAFINKEN
                     LSSDEITKLKQIVEKLK"
     gene            complement(3315..4094)
                     /locus_tag="PC700_003299"
     CDS             complement(3315..4094)
                     /locus_tag="PC700_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDF2582568.1"
                     /translation="MVSRQELKRVSKAQLSGNWGICAISMAVYIALIVLISIVGLPFE
                     PLTTIVVAVIAFCVSAGFTCMFLKITKDEKVKIGDIFVSGRIYLRSFGFYLFESIIVY
                     IYSFIITIIGAFLSFGVLLTSGAIEYLSSDNVGIGTIISTGITLVLIILVLMLPVYIL
                     LLYVSQATFLICEDKDNMGVFKSMGASLDMMKGNVLKLFVLNFSFIGWYILSIATLGI
                     GLLWLIPYVGVTNCNFYRQLVKENPDVEDILLDKEKSYTMY"
BASE COUNT         1508 a          702 c          400 g         1689 t
ORIGIN      
        1 tatctatttt tttctggtat tcttatctac tattatccgt atatttattt tatgattact
       61 cttttaacct accccctata ctttatagta atatgttttt aacctttttc attattccca
      121 atatgcttta taactacaaa tactctcccc ccttaaaaat aataataaaa ttaatcaata
      181 aatttataat catattatta ctctatgtat taagcaatta ctatgccaat aaattttgat
      241 ttttttaaac aaatcttaaa aatcctaaaa tgctaatttt ctttaacttt taatgaatct
      301 aatagataat atttaatttt cgttatattt ttcagaatgt ttcaattatt atttttaaga
      361 aaaaaaaata atgttaacat ttcttaacat acattaaaaa atgttaacat tataaatttg
      421 gtttaatact tttactttga ctcgatttac ttttattcta cttcttactt tatttttaac
      481 ttagacttta tttttttatt acttatttga gtcttattta ttttattctc actccatatg
      541 ttcaattaca tttgtctaaa ttctttgata cttttattac ataattttaa caagcttaag
      601 tgttatttgg gtagtgcagt tgataattat ttaaataatc atcaactgca tgtttatatc
      661 attacagtag cttaatgtat ttaatcatac ttacttagga ttttgaaata tcttagagat
      721 tatatttaac attagtcaat tgctgcataa ttgtaagcaa ttgaaatttg agacccatct
      781 tcattttcta tagtgatagg cttcataatt tttaatcctt cagtttttat cttttctaat
      841 tcttgttcca ttttcttaat tgtttcctca aggtcttttt gacttacatc tttgactgca
      901 ttagggtctt tagcatagtc ttttaattct tgaatatgtt tagcatactc ttcataagta
      961 taatgctcaa caccatactt ttccaattca gtagggtcag ttattcctga ttttattaga
     1021 gattcaagtt taggtaaagc aatattagat gaatcatttc catttgcaaa tactgctcca
     1081 gttcctccta gtaccattgc agataccatc attgcactta taagggattt ttttattttc
     1141 ataaccaatc ttcctttctt agagatttat ttgggtagtg tagtttaaaa tgactattgc
     1201 aatagttatc aaccacatct ttagactacc atagtagtca tatttgtgtc aagtcttttt
     1261 aaaaaacttt tcaataaaaa atactatctc aaaacaaaat taattatttt gagatagtat
     1321 tttaattcta aatcttttat attactaaga tttatggtta ctctatccat gttctatatt
     1381 gttctttatt aactgctaac aaattccctt ttcttgatat gacaaagcga ttatcattat
     1441 agttactttg ctcaattgaa acttctcctg aatcagtata aagtacatcc ttactattac
     1501 tagatttatt tacatacgtt tcttcactat tctcagaatt agttactttg ctcatagatg
     1561 attcttcatg ctctctaaat tcaattacag atacttttgc cgtactaaaa tgagtctctc
     1621 catcactttt agctttacta acaagaatat cagattctac tttgggagaa tataaatcag
     1681 ttgatttact acttattgaa aatccacttt tatcactttg ttcatttttt gttaaaccaa
     1741 aagattcaac ctcattaatc tctttatttt ctgttgcaaa aacaagacct gtcccacaaa
     1801 ctaatacagt tgaaactaaa aaagtaacat aagttgattt tttaaatttc ataatattaa
     1861 ctattctctt ttctattttt gtctttccaa atccactaca tattgtcatt gaaaatggat
     1921 tattctcctc tgctagactt attagtgtgg ttgcataatc agccctatat tcttcaccaa
     1981 gtatactcat aaccttttca tcacaactaa attctatgtc tttatttagt aaaaaaaaca
     2041 tcacccaaac taagggatta aaccaatgga caacaagagc tacaagcata aataacttta
     2101 acagattatc aaagctatta atatgaacca tttcatgagt taaaacaaag tctaaagttt
     2161 gattattagc ataattcatt tgctttggca aaataattcg tggttttaaa ataccagatg
     2221 taagtggtgt cctagtaaat tcagatatgc aaacaataaa tcttctaaaa cggctattct
     2281 tacacatcca ttttttaatg aactcatgat tttttatatg aattgaagtt ttaaaaagat
     2341 taattatttt aaaatatcta aagcataaaa gtatgctaaa cataatagta actataaaag
     2401 acaaaatgcc caacatttcc aacatcataa tattttcaat attagacatt atttcaaatt
     2461 cgtcagaatt gtttttacct gtagagtcac tagctttctt aagagtataa ttttctgttg
     2521 atgtattacc caattctaca tcaaaagtcg attcaattat actataaata ctaaaaccag
     2581 aatttaaact tactggtatt agaagtttaa ataaaacaat caaccacaat aatacaaata
     2641 cctttttagg taatttttta aataaacatg ctcttataaa aataactatt aaaagtaata
     2701 ctccagaagt aaaactcatt tctaacaatt taatgaacat aaatcttacc tcactttaat
     2761 ttttcaacaa tttgtttgag ttttgtaatc tcatcacttg aaagattttc tttattaata
     2821 aaagctgaaa gaaatttttc tgaagaccct ccaaacattt tattaattaa agtttctgtt
     2881 tcctgttttt gtacttcctc tttgcttatt aaagctttac aaacaaaatt tggctcaatt
     2941 ctctctattg caccttttga cactaatttt tttataacgg tatacgtagt gtttctattc
     3001 catcctgttt cttcttttaa aatttttact aactctccag ccttgctttc tcctctctcc
     3061 catagaactt ccattacttt aagttctgaa tcaaataatt ttatctccat ataaaatcgc
     3121 tcctcaaata tagttatcac aatagtcatt actataataa aaaactatca tagtagtcta
     3181 tactatgtca atcatttatt ttttaataat aaaaactgtc tttttaaaaa attcttataa
     3241 aagacagttt tatcaatgtt atactttaat gtttaattaa agccatattt tatttaaatg
     3301 tataactatc tatcttagta catagtataa gatttttctt tatctaatag tatatcctct
     3361 acatctgggt tttcttttac taattgtcta taaaaattac aattagtaac acctacatat
     3421 ggaattagcc atagtagacc tattccaaga gtagctattg aaagtatata ccatcctata
     3481 aaactaaagt ttagtacaaa taatttaagt acatttcctt tcatcatatc taaactagca
     3541 cccattgatt taaatacacc catattatcc ttgtcttcac aaattaagaa agtagcttga
     3601 gaaacatata atagcaatat ataaaccggt aacataagaa caagtattat aagaactaga
     3661 gttattccag tagaaataat agtccctatt ccaacattat cacttgataa atattcaatt
     3721 gctccactag taagtaaaac tccaaatgat aaaaacgctc ctataattgt gattataaaa
     3781 gaataaatat aaacaataat tgattcaaat aaataaaaac caaaacttct taaataaatt
     3841 ctcccactta caaagatatc tcctatcttg actttttcat ctttagtaat tttcaagaac
     3901 atacatgtaa atcctgctga aacgcaaaaa gctattacag ccactacaat agtagtcaat
     3961 ggttcaaaag gtaatcctac aattgatatt aatactatca atgcaatata tactgccata
     4021 gaaattgcac atattcccca gtttccagat aactgagctt tagatactcg ttttaattct
     4081 tgccttgata ccataaaaaa cacccctttc attttataaa aaaactttta aaaatataaa
     4141 taatctttta acgtataata ttaatatatc atattttcga ctatataaga tacaattatt
     4201 caccatgttt tatcaagata tgccataatt gtattactag ttttatatag tcaacattac
     4261 caaatacatc aaataaattt gattaattat tcaacaagt
//
LOCUS       DAMJWP010000255         4263 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.262, whole genome shotgun sequence.
ACCESSION   DAMJWP010000255 DAMJWP010000000
VERSION     DAMJWP010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4263
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.262"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            41..1432
                     /gene="cdtA"
                     /locus_tag="PC700_003300"
     CDS             41..1432
                     /gene="cdtA"
                     /locus_tag="PC700_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylating binary toxin enzymatic subunit
                     CdtA"
                     /protein_id="HDF2582569.1"
                     /translation="MKKFRKHKRISNCISILLILYLTLGGLLPNNIYAQDLQSYSEKV
                     CNTTYKAPIERPEDFLKDKEKAKEWERKEAERIEQKLERSEKEALESYKKDSVEISKY
                     SQTRNYFYDYQIEANSREKEYKELRNAISKNKIDKPMYVYYFESPEKFAFNKVIRTEN
                     QNEISLEKFNEFKETIQNKLFKQDGFKDISLYEPGKGDEKPTPLLMHLKLPRNTGMLP
                     YTNTNNVSTLIEQGYSIKIDKIVRIVIDGKHYIKAEASVVSSLDFKDDVSKGDSWGKA
                     NYNDWSNKLTPNELADVNDYMRGGYTAINNYLISNGPVNNPNPELDSKITNIENALKR
                     EPIPTNLTVYRRSGPQEFGLTLTSPEYDFNKLENIDAFKSKWEGQALSYPNFISTSIG
                     SVNMSAFAKRKIVLRITIPKGSPGAYLSAIPGYAGEYEVLLNHGSKFKINKIDSYKDG
                     TITKLIVDATLIP"
     gene            1485..4115
                     /gene="cdtB"
                     /locus_tag="PC700_003301"
     CDS             1485..4115
                     /gene="cdtB"
                     /locus_tag="PC700_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094607992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylating binary toxin binding subunit
                     CdtB"
                     /protein_id="HDF2582570.1"
                     /translation="MKIQMRNKKVLSFLTLTAIVSQALVYPVYAQTSTSNHSNKKKEI
                     VNEDILPNNGLMGYYFTDEHFKDLKLMAPIKDGNLKFEEKKVDKLLDKDKSDVKSIRW
                     TGRIIPSKDGEYTLSTDRDDVLMQVNTESTISNTLKVNMKKGKEYKVRIELQDKNLGS
                     IDNLSSPNLYWELDGMKKIIPEENLFLRDYSNIEKDDPFIPNNNFFDPKLMSDWEDED
                     LDTDNDNIPDSYERNGYTIKDLIAVKWEDSFAEQGYKKYVSNYLESNTAGDPYTDYEK
                     ASGSFDKAIKTEARDPLVAAYPIVGVGMEKLIISTNEHASTDQGKTVSRATTNSKTES
                     NTAGVSVNVGYQNGFTANVTTNYSHTTDNSTAVQDSNGESWNTGLSINKGESAYINAN
                     VRYYNTGTAPMYKVTPTTNLVLDGDTLSTIKAQENQIGNNLSPGDTYPKKGLSPLALN
                     TMDQFSSRLIPINYDQLKKLDAGKQIKLETTQVSGNFGTKNSSGQIVTEGNSWSDYIS
                     QIDSISASIILDTENESYERRVTAKNLQDPEDKTPELTIGEAIEKAFGATKKDGLLYF
                     NDIPIDESCVELIFDDNTANKIKDSLKTLSDKKIYNVKLERGMNILIKTPTYFTNFDD
                     YNNYPSTWSNVNTTNQDGLQGSANKLNGETKIKIPMSELKPYKRYVFSGYSKDPLTSN
                     SIIVKIKAKEEKTDYLVPEQGYTKFSYEFETTEKDSSNIEITLIGSGTTYLDNLSITE
                     LNSTPEILDEPEVKIPTDQEIMDAHKIYFADLNFNPSTGNTYINGMYFAPTQTNKEAL
                     DYIQKYRVEATLQYSGFKDIGTKDKEMRNYLGDPNQPKTNYVNLRSYFTGGENIMTYK
                     KLRIYAITPDDRELLVLSVD"
BASE COUNT         1775 a          527 c          671 g         1290 t
ORIGIN      
        1 gttcaagagt taattaaact aatattggga gggagaataa atgaaaaaat ttaggaaaca
       61 taaaaggatt agtaattgta tatctatatt gttgatatta tatctaactt taggtggttt
      121 gttacctaat aacatttatg cacaagactt acaaagctat agtgaaaaag tttgcaatac
      181 tacttacaag gctcctatag aaagaccaga agattttctt aaagataaag aaaaggctaa
      241 agaatgggaa agaaaagaag cagaaagaat agagcaaaaa cttgaaagat ctgaaaaaga
      301 agcattagaa tcatataaaa aagattctgt agaaataagt aaatattctc agacaagaaa
      361 ttatttttat gattatcaaa tagaagcaaa ttctcgagaa aaagaatata aagaacttcg
      421 aaatgctata tcaaaaaata aaatagataa acctatgtat gtctattatt ttgaatctcc
      481 agaaaaattt gcatttaata aagtaataag aacagaaaat caaaacgaaa tttcattaga
      541 aaaatttaat gagtttaaag aaactataca aaacaaatta tttaagcaag atggatttaa
      601 agatatttct ttatatgaac ctggaaaagg tgatgaaaaa cctacaccat tacttatgca
      661 cttaaaatta cctagaaata ctggtatgtt accatataca aatactaaca atgtaagtac
      721 attaatagag caaggatata gtataaaaat agataaaatt gttcgtatag ttatagatgg
      781 gaagcactat attaaagcag aagcatctgt tgtaagtagt cttgatttta aagatgatgt
      841 aagtaagggg gattcttggg gtaaagcaaa ttataatgat tggagtaata aattaacacc
      901 taatgaactt gctgatgtaa atgattatat gcgtggagga tatactgcaa ttaataatta
      961 tttaatatca aatggtccag taaataatcc taacccagaa ttagattcta aaatcacaaa
     1021 cattgaaaat gcattaaaac gtgaacctat tccaactaat ttaactgtat atagaagatc
     1081 tggtcctcaa gaatttggtt taactcttac ttcccctgaa tatgatttta acaaactaga
     1141 aaatatagat gcttttaaat caaaatggga aggacaagca ctgtcttatc caaactttat
     1201 tagtactagt attggtagtg tgaatatgag tgcatttgct aaaagaaaaa tagtactacg
     1261 tataactata cctaaaggtt ctcctggagc ttatctatca gctattccag gttatgcagg
     1321 tgaatatgaa gtgcttttaa atcatggaag caaatttaaa atcaataaaa ttgattctta
     1381 caaagatggt actataacaa aattaattgt tgatgcaaca ttgatacctt aatatttttt
     1441 cacataaata atttaatatt tttcaaattt aaggaggaga aacaatgaaa atacaaatga
     1501 ggaataaaaa ggtattaagt tttttaacac ttacagctat agttagtcaa gcactagtat
     1561 atcctgtata tgctcaaact agtacaagta atcattctaa taagaaaaaa gaaattgtaa
     1621 atgaagatat actcccaaac aatggattaa tgggatatta tttcacagat gagcacttta
     1681 aagatttaaa attaatggca cccataaaag atggtaattt aaaatttgaa gaaaagaaag
     1741 tagataaact tctggataaa gacaaatcag atgtaaaatc tatacgatgg acaggaagaa
     1801 taattccttc taaggatggt gaatatacat tatcaactga tagagatgat gtcttaatgc
     1861 aagtaaatac tgagagtact atatcaaata cacttaaagt taatatgaaa aagggtaaag
     1921 aatataaagt tagaatagag ctacaagata aaaatttagg ttcaatagat aatttatcat
     1981 cacctaatct ttattgggaa ttagatggta tgaagaaaat tataccagaa gaaaatttat
     2041 tcttaagaga ttattctaat atagaaaaag atgatccatt tatcccaaat aacaatttct
     2101 ttgacccaaa gttgatgtct gattgggaag acgaagattt ggatacagat aatgataata
     2161 taccagattc atatgaacga aatggatata ctattaagga cttaattgca gttaagtggg
     2221 aagatagttt tgcagaacaa ggctataaga aatatgtatc aaattattta gagtcaaata
     2281 ctgctggaga tccatataca gattatgaaa aagcttcagg ttcttttgac aaggctataa
     2341 agactgaagc aagagatccg ttagttgcag catatccaat tgttggagta ggtatggaaa
     2401 aattaattat atctacaaat gaacatgcct ctactgatca aggtaaaact gtttccagag
     2461 ctactactaa cagtaaaact gaatctaata cagctggtgt gtctgttaat gtaggatatc
     2521 aaaatggatt cacagctaat gtaactacaa attattccca tacaacagat aattcaactg
     2581 ctgttcaaga tagtaatgga gaatcatgga atactggatt aagtataaac aaaggagaat
     2641 ctgcatatat aaatgcaaat gttagatatt acaacacagg tactgcacct atgtacaaag
     2701 tgacaccaac aacaaattta gtgttagatg gagatacatt atcaactatc aaagcacaag
     2761 aaaatcaaat tggcaataat ctatctcctg gagatactta tcccaaaaaa gggctttcac
     2821 ctctagctct taacacaatg gatcaattta gctctagact gattcctata aattatgatc
     2881 aattaaaaaa attagatgct ggaaagcaaa ttaaattaga aacaacacaa gtaagtggaa
     2941 attttggtac aaaaaatagt tctggacaaa tagtaacaga aggaaatagt tggtcagact
     3001 atataagtca aattgacagt atttctgcat ctattatatt agatacagag aatgaatctt
     3061 acgaaagaag agttactgct aaaaatttac aggatccaga agataaaaca cctgaactta
     3121 caattggaga agcaattgaa aaagcttttg gcgctactaa aaaagatggt ttgttatatt
     3181 ttaatgatat accaatagat gaaagttgtg ttgaactcat atttgatgat aatacagcca
     3241 ataagattaa agatagttta aaaactttgt ctgataaaaa gatatataat gttaaacttg
     3301 aaagaggaat gaatatactt ataaaaacac caacttactt tactaatttt gatgattata
     3361 ataattaccc tagtacatgg agtaatgtca atactacgaa tcaagatggt ttacaaggct
     3421 cagcaaataa attaaatggt gagacgaaga ttaaaatccc tatgtctgag ctaaaacctt
     3481 ataaacgtta tgtttttagt ggatattcaa aggatccttt aacatctaat tcaataattg
     3541 taaagataaa agcaaaagaa gagaaaacgg attatttggt accagaacaa ggatatacaa
     3601 aatttagtta tgaatttgaa actactgaaa aagattcttc taatatagag ataacattaa
     3661 ttggtagtgg tacaacatac ttagataact tatctattac agagctaaat agtactcctg
     3721 aaatacttga tgaaccagaa gttaaaattc caactgacca agaaataatg gatgcacata
     3781 aaatatattt tgcagattta aattttaatc caagtacagg aaatacttat ataaatggta
     3841 tgtattttgc accaacacaa actaataaag aagctctcga ttatatccaa aaatatagag
     3901 ttgaagctac tttacaatat tctggattta aagatattgg aactaaagat aaagaaatgc
     3961 gtaattattt aggagatcca aatcagccta aaactaatta tgttaatctt aggagttatt
     4021 ttacaggtgg agaaaatatt atgacataca agaaattaag aatatatgca attactccag
     4081 acgatagaga gttattagtt cttagtgttg attagtatca aatgatttaa atttgtcccc
     4141 acaatacaat atatatttac cttataaagt actaaaaaat taactttata aggtattttt
     4201 tatttaaata ttctcttctt ctaacatatt cttttttaga actatattgt attataaaga
     4261 taa
//
LOCUS       DAMJWP010000256         4228 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.263, whole genome shotgun sequence.
ACCESSION   DAMJWP010000256 DAMJWP010000000
VERSION     DAMJWP010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4228
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.263"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(238..618)
                     /locus_tag="PC700_003302"
     CDS             complement(238..618)
                     /locus_tag="PC700_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2582571.1"
                     /translation="MYLKKLSKLELVIMKFIWNLDIKTNSYEIIDYMKEEHNLPEKVA
                     LKILSKLSKKRFLYVQETGKCMYYTVAIKEKTYLEFISRNVQNLLKNNFIRNLLVSFH
                     EEELTEEKIKSLENWVVNWEEAYI"
     gene            complement(825..2018)
                     /locus_tag="PC700_003303"
     CDS             complement(825..2018)
                     /locus_tag="PC700_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDF2582572.1"
                     /translation="MLTSLALIFLLGMASGGIFEKIKLPSLLGMLLTGIILGSYVLNL
                     IDNSILDISSDLRKIALIIILTRAGLSLDINDLKKVGRPAVLMCFIPATFEIIGMIVL
                     APKLLGVSILEAAVMGAVVGAVSPAIIVPKMLKLMEEGYGTEKSIPQMLLAGTSIDDI
                     FVIVMFTVFTGLAQGNSISAISFLQIPVSIILGVIVGAVIGICLAVFFKKVHMRDSAK
                     GVLLLSISFLMISLETALEGIVPFSGLLAVMNIGIFLQIKYRVVARRLSVKYSKLWVG
                     AEILLFVLVGATVDISYAFKAGIGAVILIFGVLLFRMVGVFFCLIKTNLTIKERLFCM
                     IGYIPKATVQAAIGGVPLAMGIASGQLILTLAVLAILITAPLGAFGIDVTYKKLLTSV
                     EGKSK"
     gene            complement(2081..3337)
                     /locus_tag="PC700_003304"
     CDS             complement(2081..3337)
                     /locus_tag="PC700_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HDF2582573.1"
                     /translation="MIEKFKTLIWIKMIIRIKSVYKTYGGLFLLGLIGIPVGAIIGLI
                     DTIFGTVLLKVTDIRETYPMYLIPFLAVVGVVIAYCYFKFGGKSSKGMNLIFEVGHGE
                     EEIIPLRLVPFIISGTWLTHLFGGSAGREGVAVQIGATFSHWVGKRLPIKNASSIFLV
                     TGIAAGFAGLFETPIAAILFAMEVLVAGSLEYQSLFPAFTASFTASAVSKTLGLEKFS
                     FALSSKVVFDLPIFWKLIVLGIIFGMVGGAFAWCLKLSKRKIGNRLKNPMIRIAIIGV
                     CLSVLFLLFYKGRYSGLGTNLIQNSFYGGEIYSFDWLLKFILTILTLSAGFQGGEVTP
                     LFSIGASLGVLLAGFFNLPIELVAALGYASVFGSATNTFFAPVFIGAEVFGYSYLPYF
                     FVVCAISYIFNMDKSIYSLQKISTKQ"
BASE COUNT         1524 a          765 c          433 g         1506 t
ORIGIN      
        1 aaaactttat ataaagaata gaaacaagtt ctagattact atagagatga catatcaaaa
       61 tatttttaaa aagtaatcta agtattagaa gtgattgaag ttagtaaata taaatgcgtt
      121 tacttttaag tattgcttaa gggaataaaa aataaaaata agctctgcaa ccatctaaaa
      181 atagttgcag agcttacaaa accaaaatta tctaaattgt aataaaaata ataacattta
      241 tatataagct tcttcccaat ttactaccca gttttctaac gactttatct tttcctctgt
      301 tagctcctct tcgtgaaatg atactaataa attccttata aaattattct tcaaaagatt
      361 ttgcacattt cttgatataa actctaaata tgtcttttct tttattgcaa ctgtataata
      421 catacacttg cctgtttctt gaacatataa gaatcttttc tttgataatt ttgataaaat
      481 ttttaaagcc actttttctg gtaaattatg ctcttctttc atataatcaa taatctcata
      541 tgaattggtt tttatatcta aattccaaat aaatttcata ataactaact ctaattttga
      601 taattttttt aaatacatag ttatcactcc gcttctttat ctatataatt aaatattaca
      661 ataacaattt cacaaaatca attctaataa tcatcacatg ccttaaaatc cgatataaac
      721 ttctaccttc tgtaatcact caaatgattt tctaacattg tactcaataa tatagttttt
      781 tccaaaatat aatttatcac ttaaaataat ctttattttt attcttactt actcttacct
      841 tctacagaag ttaataattt tttataagtt acatcaattc caaaagctcc aagaggtgca
      901 gttataagta tagcgagaac cgctaatgtc aaaataagtt gtccagatgc tatccccatt
      961 gcaagtggta cacctcctat tgctgcctgt actgtagcct ttggaatata gccaatcata
     1021 cagaacaatc tttcttttat tgtcaaattt gtcttaataa ggcagaagaa tactcctacc
     1081 atacggaata aaagaactcc aaatattaaa attacagctc caattcctgc tttaaatgca
     1141 taacttatat ctactgttgc ccctaccaaa acaaacaaga gaatttcagc accaacccat
     1201 agttttgaat actttacaga caatcttctt gcaactacac gatattttat ctgaaggaaa
     1261 atcccaatat tcataactgc taataatcca gaaaatggca caataccctc taaagcagtc
     1321 tccaaactta tcattaaaaa tgagatactt aaaagcaaca cccctttagc agaatctctc
     1381 atatgaacct tcttaaaaaa tactgctaaa cagattccaa tcactgctcc aactattaca
     1441 cctaatataa ttgatactgg aatttgtaaa aaactaatag ctgatatact atttccttgt
     1501 gccaatcctg tgaatactgt aaacatcaca attacgaaaa tatcgtctat agaagttcct
     1561 gctaaaagca tctgtggaat acttttttct gtaccataac cttcttccat taatttaagc
     1621 attttaggta caataattgc gggagaaact gctcctacta ctgcacccat gactgctgct
     1681 tccaatatag aaacgcctag taattttggt gctaaaacta tcattccgat aatttcaaaa
     1741 gtagctggaa taaagcacat aagtacagca ggtcttccca ctttcttcaa atcgtttata
     1801 tctaaagata atccagccct tgttaatata ataatcaatg caatctttct aaggtcagaa
     1861 gaaatatcta gtattgaatt atcaatcaaa tttaaaacat atgaacccaa tatgattcct
     1921 gtaagtaaca ttcccaataa gctaggtaat tttatcttct caaaaatacc tcccgaggcc
     1981 attcctaata aaaaaatcaa agctaaactt gtcaacatat tcctacccta tcctttctct
     2041 aatattcttt ctcttataat catacattat gttgttttat ttattgtttc gttgaaattt
     2101 tttgtagtga ataaatagat ttatccatat taaatatata tgaaatagcg catactacaa
     2161 agaaataagg taaatagctg tatccaaaaa cttctgcccc tataaataca ggagcaaaaa
     2221 aagtatttgt agcactgcca aagacgctag catagccaag cgctgcaact aattcaattg
     2281 gcaaattaaa aaatcctgct aacaatacac caagactagc tccaatggaa aacaaaggtg
     2341 taacctctcc tccttgaaaa cctgctgaca aagtcagtat tgtaagtata aactttaaca
     2401 gccagtcaaa tgaataaatt tctccaccat aaaaactatt ttgaattaaa tttgtcccta
     2461 aacctgaata tcttccttta taaaataaaa ggaataatac actaagacat actccaataa
     2521 tagcaatcct tatcatagga ttttttaatc tattacctat ttttcttttt gacaatttca
     2581 ggcaccatgc aaatgctcca ccaaccattc caaaaataat tcctaaaaca ataagcttcc
     2641 aaaatattgg taagtcaaat acaactttag aagaaagtgc aaaagaaaat ttctctaacc
     2701 ctaaagtttt tgatactgca cttgctgtaa aagaagcagt aaacgcagga aaaagcgatt
     2761 gatactccag tgaaccagct accaaaacct ccatagcaaa tagtattgct gctattggtg
     2821 tctcaaataa tcctgcaaaa ccagctgcta taccagttac tagaaaaata ctagaagcat
     2881 tttttatagg caacctttta cctacccaat gtgaaaaagt tgcaccaatc tgaacagcaa
     2941 ctccttctcg tcctgcacta ccaccaaata aatgggttaa ccatgtacct gatattataa
     3001 atggaaccaa tcttaaagga ataatttctt cttctccatg ccctacctca aatattagat
     3061 tcattccttt actacttttt cctccaaatt taaaatagca ataagcaata acaactccta
     3121 ctactgctaa aaatggtatc agatacattg gatatgtttc tcgaatatct gtaactttta
     3181 gaagaaccgt tccaaatatg gtgtctataa gacctataat tgcaccaact ggtataccta
     3241 tgagtcctaa caaaaatagt ccaccatatg ttttataaac gcttttaatt cttattatca
     3301 ttttaatcca tattaaagtt ttaaattttt ctatcatttt aattcctttc tatttgcatc
     3361 ataagatttt ttgaaagttt tattgcatca actgtagtca atgtaatgac tatcataatt
     3421 gttttttaaa ttgataggat ataggtatac tcttaaccca ttcttaataa aataattaac
     3481 atataatcta ctgttttttc tattaattaa atatttcttc aaatttaaaa aataaaaatt
     3541 tagtaagtaa ttcacctcca ttttatcgat ttaaacaaca aaaaaagctg gtggcatcta
     3601 tgatatcata gaagtcatca gctcattggc agtttaggat attatcatcc aagggagaac
     3661 ctcattccca taattattat ataatttttt atttttcata tgtctatttt atgtaaattg
     3721 tatttttaat gttataaata atgtaaccta tcaattaaaa tattaaagcc tccaatatta
     3781 tgagtgttca atatttcata ttatttttat gatatataat ttaaatgatt ttctttactt
     3841 tgtattatga tacataactt aaatatttta cctcactcta cattatgata tattatttaa
     3901 atatttttct tcactctgct tatattatga tacatgattt aaatattttt actcacttta
     3961 tattataata catgctttaa ataatcttat tcgctttgta ttataacaca ttatttaaat
     4021 atttttactc actctgcatt atggtacatt atttaaatat ttttcttcgc tttgcattat
     4081 tatacatgct ttaaatattt ttactcacct tgtattatgg tacattatct aaatattttt
     4141 cttcactctg tattatgata tataacttaa atattttacc tcactctaca atattatata
     4201 taatttaaat atttttcttc actttgta
//
LOCUS       DAMJWP010000257         4221 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.264, whole genome shotgun sequence.
ACCESSION   DAMJWP010000257 DAMJWP010000000
VERSION     DAMJWP010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4221
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.264"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            141..1394
                     /locus_tag="PC700_003305"
     CDS             141..1394
                     /locus_tag="PC700_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HDF2582574.1"
                     /translation="MLNKKNISSIIMTMILSLSTTVFVFADENTNVKGQDVTKYNEEM
                     VSKNLEDLSIEQLIDIRDKAIDKNLEGLRKFGEDSVKERKDSILNFEFGLVAFVNKNY
                     SNLTEKEKLIRQINIVEYENSINDIMDPSGDFFKVTNQSYQINEFVLKNGKNYKEWSY
                     EYVEFLIEHLEDLCNFVDFDAKYVIEMVDPSIKPDLSDNQKKSLNNELIKMYSSNTLS
                     TNIKDEIENWENNLNINEKNSNEPLPRVSPSSYRQQALAYAKKHGYTNGYYGSTNRKN
                     NAPSPYYNFQKDGYGDCANFVSQCLHEAGVTFWTNNNPWYYYSTSNRSPSWAGAREFK
                     IHWMNRIAYQTLTVSNSLSFLRPATPVSILNSSGVATHTLISTDKHSNGYNFSYAAHS
                     DEGARTNLLKKLEGKKISYYKVYWE"
     gene            1676..4021
                     /gene="secA"
                     /locus_tag="PC700_003306"
     CDS             1676..4021
                     /gene="secA"
                     /locus_tag="PC700_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDF2582575.1"
                     /translation="MSVLDAILDKADEQEIKKLNLIVDKIESLEKDMEIKENEQLKEM
                     TNTFRLRLDKGESLDDILPEAFAVVREVSKRVLGMRQYKVQLIGGIVIHQGKIAEMKT
                     GEGKTLVEVAPVYLNALTGKGVHVITVNDYLAERDKELMRPVYEFLGLTVGVILSNQD
                     PSIRKEQYKCDITYGTNSEFGFDYLRDNMVPDLSHKVQRELNFAIVDEIDSILIDEAR
                     TPLIIAGDGDEDLKLYELANNFVKTVKEEDFEMDRKDKTIALTASGISKAESFFGITN
                     LTDIKNIELYHHINQALRGHKLMEKDVDYVISNGEVMIVDEFTGRVMDGRRYTDGLHQ
                     AIEAKEGVEIKNESKTMATVTYQNFFRLYEKLSGMTGTAKTEEGEFESIYKLNVVQIP
                     TNRPVIRADLHDKVFKTEEEKYSAVVEEIIRIHKTRQPILVGTVSVEKSEKLSKMLKK
                     QGIKHQVLNAKQHDKEAEVISKAGKLDAITIATNMAGRGTDISLGAGDKEEEEKVKNL
                     GGLYVIGTERHESRRIDNQLRGRSGRQGDPGTSRFFVSLEDDVIKLYGGKTIEKLMKR
                     TSSKENTAIESKALTRAIERAQKGVEGKNFEIRKNVLKYDDTINEQRKVIYNERNKVL
                     NDEDIKEDIQKMVKDIIQEAGETYLIGRKRDYYGYFKHLYSTFMPADTLLIPGVDKKS
                     VQEIIDSTYEISKRVYDLKKMMIGIDKVAELEKTVLLKVVDQYWIDHIDAMEQLRQYI
                     GLKSYAQKDPFKEYALEGYDMFEALNKNIREATVQYLYKFN"
BASE COUNT         1760 a          421 c          760 g         1280 t
ORIGIN      
        1 ctagaaaggg aggattatat tatgatattt agtaaacgct cttttagaaa aaaacggtga
       61 taaacttgtt ttaaatttta gtagaatatg atttttgttt tggcaagtat taatacatat
      121 attaaagagg gaggtttaat atgttaaata aaaaaaatat aagttcaatt attatgacaa
      181 tgattttaag cttatcaact actgtatttg ttttcgctga tgaaaatact aatgtgaaag
      241 gacaagatgt aaccaaatat aatgaagaaa tggtctctaa aaacttagaa gatttaagta
      301 tagaacaatt aatagatatt agagataaag ctattgataa aaatttagaa ggtcttagaa
      361 aatttggaga agattcagtg aaagaacgta aagattcaat tttaaacttt gaatttggat
      421 tagtagcttt tgttaacaaa aattatagta acttaacaga aaaagagaaa ttaataagac
      481 aaatcaatat tgttgagtat gaaaattcta taaatgatat aatggatcct tcaggcgatt
      541 ttttcaaagt aacaaaccaa agttatcaga ttaatgaatt tgtattaaaa aatggaaaaa
      601 actataaaga gtggtcgtat gaatatgtag agtttttgat tgagcattta gaggatttat
      661 gtaattttgt agattttgat gcaaaatatg taatagaaat ggttgatcct tcaataaaac
      721 cagatctttc agataatcaa aagaaatctc taaataatga attaataaaa atgtattcaa
      781 gtaatactct aagtacaaat ataaaagatg aaatagaaaa ctgggaaaat aatttaaata
      841 ttaatgagaa aaattcaaat gaacctttac caagagtaag cccttctagt tatagacaac
      901 aagcattagc ttatgctaaa aaacatggct atactaatgg ttattatggt agtacaaata
      961 gaaaaaataa tgcaccttct ccatattata attttcaaaa agatggatat ggagattgtg
     1021 ctaactttgt ttcacaatgc ttacatgaag ctggagtaac attctggacg aataataatc
     1081 catggtatta ttattctact agtaatagat caccttcttg ggctggagca agagaattta
     1141 aaatacattg gatgaatcga attgcatatc aaacacttac tgtatcaaac tctttaagtt
     1201 ttttaagacc agcaacacct gtaagtatat taaatagtag tggagtagca actcatacac
     1261 taatatctac agataagcat tcaaatggat ataatttttc atatgctgca cattcagatg
     1321 aaggtgcaag aacaaatttg ttaaaaaaac ttgaaggaaa gaaaatttct tattacaaag
     1381 tttattggga ataaataaaa atttatactt ttaattaaat gaaaattgaa aagaataaat
     1441 atctatttca agatccaata agtatttagt tgaagcagct attaaatttt attacataat
     1501 cataaaaaag agactagtgg aatttagtct cttttttatg tgagaaatac ttaaatatat
     1561 aaagatgtaa tttatttaag tatgattatt atactataca ataaaataat tgtctactat
     1621 tctataattt taaggtataa taataattgt atgataaaag aggaggtcaa aaaatatgtc
     1681 agttttagat gcaatcttag ataaagcgga tgaacaagaa attaaaaaat taaatttaat
     1741 agtagataaa atagaatctt tagaaaaaga tatggaaatc aaagagaatg aacaattaaa
     1801 agaaatgaca aatactttta gacttagatt agacaaaggt gaaagtttgg atgatatact
     1861 tccagaagca tttgctgttg ttagagaggt ctcaaaaaga gttttaggaa tgcgtcaata
     1921 taaagttcag ttaattggag gtatagtaat tcatcaagga aagatagctg agatgaagac
     1981 tggtgagggg aaaactttag ttgaggtagc tccagtatat ctaaatgctt taacaggaaa
     2041 aggtgtacat gtaattacag taaatgatta tttggcagaa cgtgataaag aacttatgcg
     2101 tccagtatat gaatttttag gattgactgt aggtgtaatt ttatctaacc aagacccaag
     2161 tataagaaaa gaacaatata aatgtgatat aacttatgga acaaatagtg aatttggatt
     2221 tgactattta agagataata tggtgccaga tttgtctcat aaagtacaaa gggaactaaa
     2281 ctttgccata gtagatgaga tagactcaat attaatagat gaagctagaa ctccacttat
     2341 tattgcagga gatggtgatg aagatttaaa actttatgag ttagcaaata actttgtaaa
     2401 aactgttaaa gaagaagact ttgagatgga tagaaaagat aaaactatag cattaacagc
     2461 aagtggtata agcaaagctg agtcattttt tggtataaca aaccttactg atataaagaa
     2521 tatagaatta tatcatcata taaatcaagc tttaagaggt cataagctta tggaaaaaga
     2581 tgttgactat gttatctcaa atggagaagt aatgatagtt gacgaattta caggaagagt
     2641 aatggatggt agaagatata cagatggact tcaccaagct atagaagcca aagaaggtgt
     2701 tgagataaag aatgaatcta aaactatggc tactgtgact tatcaaaatt tcttcagact
     2761 atatgaaaaa ctttctggta tgactggtac tgcaaagaca gaagaagggg aatttgagtc
     2821 aatctataaa ttaaatgttg tccaaatacc aactaataga ccagttatta gagctgattt
     2881 gcatgataag gtatttaaaa cagaagaaga aaagtatagt gctgttgtag aagaaataat
     2941 aaggatacat aagactagac agccaatact cgtgggaaca gtttctgttg aaaaatctga
     3001 gaagttatct aaaatgctta agaagcaagg tattaagcat caagtcttaa atgctaaaca
     3061 acatgataaa gaggcagagg taatttctaa agctggtaaa ttagatgcta taacgattgc
     3121 tacaaatatg gcaggtagag gtacggatat ttctctaggt gcaggagata aagaagaaga
     3181 agaaaaagtt aaaaatttag gtggtcttta tgttatagga acagaaagac acgaatcaag
     3241 aagaattgac aatcagctta gagggcgttc tggtcgtcaa ggagacccag gtacatcaag
     3301 attttttgta agtcttgaag atgatgtaat aaagctttat ggtggaaaaa ctatagagaa
     3361 acttatgaag agaacaagtt caaaggaaaa tacggctatt gaaagtaaag cacttacaag
     3421 ggctatagaa agagcacaaa aaggtgtaga aggtaagaat tttgaaataa gaaaaaatgt
     3481 tcttaaatat gatgatacta ttaatgagca aagaaaggtt atatataatg aaagaaataa
     3541 agtgttaaat gatgaagata taaaagaaga tattcaaaaa atggttaaag acatcataca
     3601 agaagcagga gaaacttatt taattggaag aaaaagagat tattatggat attttaaaca
     3661 tttatatagt acatttatgc ctgcagatac attactaata cccggtgtag ataaaaaaag
     3721 tgtccaagag ataattgata gcacatatga aatttcaaaa agggtttatg acttgaaaaa
     3781 gatgatgatt ggtattgata aagttgcgga gttagaaaaa acagtacttt taaaagtggt
     3841 tgatcaatac tggatagatc atatagatgc tatggaacag ttaagacagt atataggtct
     3901 taaatcttat gctcaaaaag atccgtttaa agaatatgct ttagaaggat atgatatgtt
     3961 tgaagcttta aataaaaata taagggaagc aacagtacaa tacttatata aatttaacta
     4021 aaaaagttca tgtaattttt attaaatgaa aaataaaata ttttgattct ttatattgta
     4081 attacaatta aataattaag gaatacaaat gaaaaagaag ctgaaaatca gcttcttttt
     4141 ctgtgtgaga aatacggaaa aaagagatgc ttgaaatttc tttcttccat ttaatattat
     4201 actataaaga agatgaaaag t
//
LOCUS       DAMJWP010000258         4178 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.265, whole genome shotgun sequence.
ACCESSION   DAMJWP010000258 DAMJWP010000000
VERSION     DAMJWP010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.265"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            133..471
                     /locus_tag="PC700_003307"
     CDS             133..471
                     /locus_tag="PC700_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2582576.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
     gene            493..810
                     /locus_tag="PC700_003308"
     CDS             493..810
                     /locus_tag="PC700_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2582577.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            960..1637
                     /locus_tag="PC700_003309"
     CDS             960..1637
                     /locus_tag="PC700_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582578.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     LYYIYNH"
     gene            complement(1965..2732)
                     /locus_tag="PC700_003310"
     CDS             complement(1965..2732)
                     /locus_tag="PC700_003310"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDF2582579.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKVAVMPQNERDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            3538..4170
                     /locus_tag="PC700_003311"
     CDS             3538..4170
                     /locus_tag="PC700_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HDF2582580.1"
                     /translation="MTIPNSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVSPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
BASE COUNT         1692 a          524 c          520 g         1442 t
ORIGIN      
        1 gactatacaa aaataaatag cacttatttc agtataaaaa catatatagt ctatatttag
       61 actaaaaaag tataaatagt ctatataaaa caacttaaac tatgaatata atctaaagtg
      121 gagggataat aattggcaaa tataggaaaa ataataggag atagaataaa agtctataga
      181 acaaaattgg gatatagtca agagtttcta gcagaaaagg caggtttaca tccaacatat
      241 attggtcaga ttgaaagagc tgagacaaat acatctatca atattataat gaaaattgct
      301 actgctttag acataccact tgaattgtta tttgcaaata ttattacaac agaaggcgtt
      361 gataactctg tacctttaga atgttatgaa atgattaata aattaactga aaaagaacaa
      421 attgcaatgc tagagttaat aaagtgcatc attaaataca aagaaatata agtgagggaa
      481 atagtaacaa taatgaatta tataggtaaa agacttaaag aagaacgaaa aaaagcaaat
      541 ttaacttcaa aagaatttgc aaatatggtt ggagtatcgc cttggtatat aactcaaatc
      601 gaaagtggta aaaagaatcc aagtcttaaa acattcataa aatttgtaaa tatattaaat
      661 atttctgcag atgttcttat aaaagatatt acttccactg gaaaaacata tttagaaaat
      721 gatataaatg aagagttaaa agacttaaat tcaagagaac taaatctgat atcacagatt
      781 atagacatca taaaaaagaa taaaatataa ttctttatat tttatataaa aaaaaaggtt
      841 atttgaaata aataaccttt ttttgtcttt ttgtgttaac attgtcttat agtgctaatg
      901 agtactatta gaccatgttc tatggcctta ttttaatata aaacgattgg agataggcta
      961 tgattgtgat tgaaggtagt gataaattta agattgcaaa agaatatatt gatgtagaat
     1021 atactctttt tagcaaagta acttttaggt atgaaaagtt gaaatttaaa gataatgctg
     1081 aattggaaaa aattaagatg tttaaatata aaaatggcta catccctaat aaattaaacc
     1141 tttcttttgg atatggattc tcttcttata aaaagcaaat aattagagaa actgtagata
     1201 ctttaagatt gacagaaatt ttttcaagcg agaacataga agatattaaa tttataaaag
     1261 atggtacaaa aaaattagaa attagcatag agaaagttgt gaaatttaaa cgtcgaaaaa
     1321 aaagaaatta tgtttgttgc tattgccctg atatgtatag agatataaaa ctcgacaaag
     1381 aatctatcaa taagatatac aacagaaaaa taaaaataga aagagaagtt aatatttttg
     1441 aagatgagga tgttataata aacaagaaag tattgaagtt tccaaagtct tggacaaaga
     1501 atatgcaaaa atattggtta agtgaaaata agtatcccat acattctact gtaattgatg
     1561 atgatagata taaatgttgt aatgtacaat atacaaaaaa tagagtgata atattatatt
     1621 acatatataa ccattaatat acataattac aaaaaaataa tgacaaatat acataataca
     1681 aatatatctt ctactttaga taaaacagtt attatgaaaa cttactatct ataaataata
     1741 gaaatgctag tataccatct ataatctaat gtctagtagg ttttaatggc atattttaaa
     1801 tatgccatag ttgatttata agattttcta tatcactttc aaaatacaca ctaaatagtt
     1861 gcttttcaga tataattgcc caatttaaat tttactatct gaaaagtttt ttatatttaa
     1921 tttatatctt caaaaatacc gtctaatatt tctatataat taatttaatt aatactctta
     1981 tgtaatagat taagtactga atttaactct ttaaatgata tttgccctgg tgctgaaaca
     2041 ctttttgcag ctccaaaagt caatgcagaa ccaaatattt caccacacaa tctacttata
     2101 acacccattc ctgacattga cattgttatt attggtctat ctgcataaat tttaaacatt
     2161 tcatttgtag cttcaagtag cactaacaca tctctttcat tttgcggcat aacagctact
     2221 ttgggtaaat ctgcgcccaa ttcctgcatt ctacataaac gagatacaat ctcttctttt
     2281 tttggagttt tattaaaatc atgattagat ataattactt tcacttcttt tttatgagca
     2341 aaattaacta cttcatctat gacctcatct cccataaata actctacatc tattaaatct
     2401 actaaacctg tattagaaat ttctttattc aaagtagtat aataatctct tgatattaat
     2461 ttttctcccc cttctactac acttctaaat gtgaataata aagggatatc atgtatataa
     2521 cttcttaatt catagagaac ttccttaact tctttaatat tttctacatt ctcaaaaaaa
     2581 tcaactcgcc actctataat atccaaacat gcatctttta actctttagc ctcttttata
     2641 atatcttttt tatttttccc aataattggt acacaaattt taggtctacc ttctccaata
     2701 gtaatatttt ttacttgaac ttttcttttc atattctcct ccataatcag aaattctata
     2761 ggactttaaa ttctattttc tgcataattt aattatacat taaaaatact atacattata
     2821 aaaatactaa tttaaaaata atttttattc taattcttaa aacaatccca catattttag
     2881 aaaaggtact ctataatata caaacacttc ttttttacct aaatttattt taacattcta
     2941 taattttcac aatattcttt aaattttact catataaaca atattattct ggatgctaat
     3001 agaattaatc tataaatcat agtataatta tgcagaaata actcgaattt cactacttat
     3061 aaagagattg aatattaatg aatctaaatc atttgcatta cttcagagtt ttgactaaag
     3121 ttgaacacca tacaaaagta gctactcaat tgtttatacc ccaaccaagc ttaagtaatg
     3181 ttatatagat tcttgaaaaa gaatttggta tctttttatt tgaaaaacaa ggtagaaatg
     3241 tttccctcac taaatacgga agtttttttc taacctatgt tgataaagca ttaggttaat
     3301 tagaaaaagt ttacatataa aatatattaa attgttgcaa gttttattgt tttgatatac
     3361 aatcaactaa acatataatc tttataaaat actatacagt taaaagtctg ttttaaaaaa
     3421 aaatgtataa attataaatc aaaatataga tactatcaag gagtgattct gtgaagagat
     3481 gtattgtagt agtggaatag tcaaaaacat aatatatatt aaaggagaga tttaaatatg
     3541 actattccaa attcaaataa ggtatatcca agaagtaatg attatcaaac tgtatatctt
     3601 aaaaatgtaa taacaaggga aaatatagaa gttggagatt acacaatata taatgacttt
     3661 tataatgacc caagagattt tgagaaaaac aatgtattat atcattaccc tataaacaat
     3721 gataaattaa taattggtaa gttttgctca atagcatgta atgcaaaatt tctaatgact
     3781 agtggaaatc actcaatgaa atcaaaatcc acatatactt ttcccatctt ttatgaagaa
     3841 tggaaactcg atatcaacca tataacggac gcttgggaca ataagggtaa cattataatt
     3901 ggaaatgatg tgtggatagg ttatgattct ataattatgt caggtgtaaa tattggtgat
     3961 ggtgcaatta ttggaacaag agcagtagtt actaatgacg tctcaccgta ttctattgtt
     4021 ggaggagtac ctgcaaaaat tataaaaaag agatttaata atgatacaat ttcaaaatta
     4081 ttagaaatta aatggtggga ttggccatat aaaaaaattc aatcaaacat taagcatatt
     4141 caatcaggaa atattgataa attagtttaa tattttat
//
LOCUS       DAMJWP010000259         4121 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.266, whole genome shotgun sequence.
ACCESSION   DAMJWP010000259 DAMJWP010000000
VERSION     DAMJWP010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.266"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            352..1626
                     /locus_tag="PC700_003312"
     CDS             352..1626
                     /locus_tag="PC700_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDF2582581.1"
                     /translation="MQFMDVSKYIEGANLKVKTKNGETVKRIYFNNSATPLVLKNVVD
                     NLNCEIPWLTYINAPGIISEKNTLKYENVRSTILKLIDGDEEKDSAIYVKNATEGINF
                     LAKFFKDENPDKFIITTAMEHMANYLPFKVNFPTKVVGITEKGELDLCQLENILKNYA
                     GNVSLVTVTGASNVTGITTPIYEIARIAHKYGAKILVDIVQVIQHKPFSMMPHECDEH
                     IDFVVFSAHKCYSPLNGGALVGPKKFFEKFNPVLYGSGSTKFVNDNKILYANVPQRFE
                     AGYPEYFGTLAMGKALNTLNKIGLNRISKYERELFLYAKEEMSKIPNIIIYGDKSNNV
                     IIPNISFNISNMYYKDVAKYLVDNFGIETGAGVVGADIYVQKLLGISPKEAYVRFMRE
                     NPVGLVRISLGMYNTFDEVDSFIHAIKTLAFK"
     gene            complement(1833..2150)
                     /gene="trxA"
                     /locus_tag="PC700_003313"
     CDS             complement(1833..2150)
                     /gene="trxA"
                     /locus_tag="PC700_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDF2582582.1"
                     /translation="MVKIINNNEFINEVENKDGLVVVDFFATWCGPCKMLSPIYEALG
                     NEMVEKANFLKVDIDQSIELAQKFEVSTVPTMLIFKDGKPVDRLIGFMPKENLKNKIE
                     SYM"
     gene            2417..3169
                     /locus_tag="PC700_003314"
     CDS             2417..3169
                     /locus_tag="PC700_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HDF2582583.1"
                     /translation="MDNIINELQNIGFTKYESQIYISLLKESPLTGYEISKLSGVPQS
                     KVYENITKLLNNNIIINVGTDPIKYIPINPNELLKNKKKEFDNSMDNLKNSLQILNKN
                     KSVEYVLNIKGDKNILKKALEMIKNAKQEIIISCPYEEILELKKELLIAYKKNINIEI
                     LLLENKSLKGLDNIHTHGGNKTTLENKMLYKGIILIVDNDEILTGNLSEGSEAISIWT
                     KNSNILYIGVQYIKHEIYISEQIKGGYKNEQN"
     gene            3156..4037
                     /locus_tag="PC700_003315"
     CDS             3156..4037
                     /locus_tag="PC700_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HDF2582584.1"
                     /translation="MNKIKIKQVDAFTTIPFGGNPAGVVTDASNISDEIKQNVAKEMN
                     LSETAFVSESNVADFKVQFFTPGLEVDLCGHATIGTFHALLEEGRLNTNKSIFYQETK
                     AGVLSVELKEINNENIFMMEQNTPEFENLDNYRKELAQMIGVHEDRLLEYPIIKVNTG
                     LWWLVFGLKSLDDLKNISPDLNKIKKFSGDNNIIGITPFCIETIDKKCDYHLRSIAPY
                     VGVTEDPVCGTGNACVSSYIVHHNILDKTSFIGEQGNFINRPGKVYVNITRENNDITS
                     VKIGGSAYTVLSGEIMY"
BASE COUNT         1616 a          541 c          566 g         1398 t
ORIGIN      
        1 tcaatttctt tactattttt acaactttat tagtcatatt cataattatt tctaattagt
       61 gtttaacatt ttatgtaaaa tttataaaaa aatgtatata aattaaaata agtgactata
      121 ctaaaaatat gatacggata aacaataagt tcaacctatc tcccccaaaa tcaagtgagc
      181 tacagtttcc cccaactgta gctcattttc gtgtaattta ataaacatct caacaagaat
      241 taattaacaa aaccccttat aaaaacataa catatattaa acaagctctt gattaaaact
      301 tgagcttttt taataaaaaa cagaatagtt tattagataa ggggtttttt tatgcaattc
      361 atggatgtat ctaaatacat tgaaggtgca aaccttaaag ttaaaactaa aaatggtgaa
      421 actgttaaaa gaatatattt taacaactct gccactccat tagttttaaa aaacgtagtg
      481 gataacttaa actgtgagat tccttggctt acttatatta atgctccagg aataatctca
      541 gaaaaaaata ctttaaaata tgaaaatgta cgctctacta tcctcaaact tattgatgga
      601 gacgaagaaa aggattctgc tatttatgtc aaaaatgcta cagaaggaat aaacttctta
      661 gcaaaatttt ttaaagatga aaatccagat aaatttatta ttaccacagc aatggagcat
      721 atggctaatt acctaccttt taaagttaat tttcctacaa aagttgttgg aattacagaa
      781 aaaggagaac ttgatttatg tcaattagag aatatactta aaaattatgc tggtaatgta
      841 tctctcgtta cagttacagg agctagtaat gtcacaggta taaccactcc tatatatgaa
      901 atagcaagaa tagctcataa gtatggtgca aagatattgg tggatattgt gcaagtaatt
      961 cagcataaac ctttttcaat gatgccacat gaatgtgatg agcatataga ttttgttgtt
     1021 tttagtgctc ataaatgtta ttctccacta aatggaggag ctctggtagg acctaaaaaa
     1081 ttctttgaaa aatttaatcc tgtactatat ggctcaggtt ctaccaaatt tgtaaatgat
     1141 aacaaaatac tatatgcaaa cgtaccccaa cgttttgaag caggataccc agagtatttt
     1201 ggaactcttg caatgggaaa agcactgaac actttaaata aaattggtct aaacagaatt
     1261 tcaaaatacg aaagagaatt atttttatat gctaaagaag aaatgagtaa aatacctaat
     1321 ataataatat atggagataa atcaaataat gtaattatcc caaatatatc ttttaatata
     1381 agtaatatgt attacaaaga tgtagcaaaa tatttagttg ataactttgg aatagaaact
     1441 ggagctggag tagtaggtgc agatatttat gtacaaaaat tattaggtat aagtccaaaa
     1501 gaagcatacg tcagatttat gagggaaaat cctgtaggtc ttgttaggat aagcttagga
     1561 atgtacaata cttttgatga ggtggatagc tttatacatg ctattaaaac tttggctttt
     1621 aaataagctt tttctattct tttttatcaa taaatagaga gtagttttgt tttcacaaaa
     1681 aaacaaatta tatcaacagt caatttcatt agcaattaga cactcatttg taactataca
     1741 aactatttta cactatacaa ataaaaaact cattacaatg agtttttttg tttatatatg
     1801 atatttattt taattacttc aatttacttt aattacatat atgattcaat tttgtttttt
     1861 aaattctcct taggcataaa acctattaat ctatctacag gttttccatc cttaaatatt
     1921 agcatagttg gtactgtaga tacttcaaat ttctgagcta attctatact ttggtctata
     1981 tccaccttca agaagtttgc tttttcaacc atctcatttc ctaaagcttc atatattggt
     2041 gatagcatct tacaaggtcc acaccaagtc gcaaagaaat ctacaactac taaaccatct
     2101 ttattttcta cttcatttat aaattcatta ttatttatta tttttaccat aatatacatc
     2161 tcctttatta tcttcttgat actaatatac tacgccctat ataacagttc aattaggcac
     2221 ttttttgtaa ccgcctattt ttaatagatt taatattatg ataaatagat ttataagatt
     2281 aaatatttat aagactaaat atttctgata tattttattt ttttcttaat atagttgaca
     2341 ttttctccct taaattataa aatcaaatta ataactacat aggtagttac taatctttta
     2401 tcaaaggagt ttctatatgg ataatataat taatgaatta caaaatatag gatttactaa
     2461 atacgaatct caaatataca tatcattatt aaaggaaagc cctttaacag gatatgaaat
     2521 aagtaaatta tcaggtgtac cacaatcaaa agtctatgaa aatataacaa agctattaaa
     2581 caataatata attatcaatg ttggaactga ccctattaag tacataccca taaaccctaa
     2641 tgaattatta aaaaataaga aaaaagaatt tgataattct atggataatc taaagaatag
     2701 cttacagata ttaaataaaa ataaaagtgt agaatacgtt ttaaacataa aaggagataa
     2761 aaatatatta aaaaaagcat tagaaatgat aaaaaatgca aaacaagaaa ttataatttc
     2821 ttgcccttat gaagaaattc ttgaattaaa aaaagaactc ctaattgctt acaaaaaaaa
     2881 tattaatatt gaaatactac ttttagaaaa caaaagtctg aaaggtcttg ataatattca
     2941 tactcatgga ggtaataaaa ctacacttga aaataaaatg ctttacaaag gaattatttt
     3001 gattgtagat aatgatgaaa tattgacagg aaacttatca gaaggcagtg aggccatttc
     3061 aatttggaca aaaaactcta acattttata tattggagtt caatacataa aacatgaaat
     3121 ttatatttca gaacaaataa aaggaggtta taaaaatgaa caaaattaag attaaacaag
     3181 ttgatgcttt tacaactata ccttttggtg gaaatcctgc tggtgtcgtt actgatgcct
     3241 ctaacatttc tgatgaaatt aagcagaatg tggctaaaga aatgaattta tcagaaactg
     3301 catttgtttc agagtcaaat gttgcagatt ttaaggtaca atttttcaca ccaggtttag
     3361 aagttgattt atgtggtcat gcaacaatag gtactttcca tgcacttttg gaagaaggaa
     3421 ggcttaatac taataagagt atattttatc aagaaacaaa agctggtgta ttatctgtag
     3481 agcttaagga aataaataat gaaaatatat ttatgatgga acagaataca cctgaatttg
     3541 aaaatttaga taattacaga aaagaacttg cacaaatgat aggtgtgcat gaagatagac
     3601 ttcttgaata tcctattata aaggtaaata ctggactttg gtggcttgta tttggtctaa
     3661 aatctttaga tgatttaaaa aatatctctc cagatttaaa taaaatcaaa aaatttagtg
     3721 gtgataataa tataattggt ataactccat tttgtataga aactatagat aaaaagtgtg
     3781 actaccattt aagatctata gcaccatatg ttggagttac agaagaccct gtatgtggaa
     3841 ctgggaacgc ttgcgtatca tcttatatag tacatcacaa tattttagat aaaactagtt
     3901 ttattggtga gcaaggtaac tttataaaca gacctggaaa ggtatatgta aatataacta
     3961 gagaaaacaa tgacataact tctgtaaaaa ttggaggaag tgcttatact gttttaagtg
     4021 gtgaaattat gtattaatcc catacctaat aaaaacaaat aaaaatacta gctaactaaa
     4081 gctagtattt ttatttgtta tttttattta ataatcacac t
//
LOCUS       DAMJWP010000260         4105 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.267, whole genome shotgun sequence.
ACCESSION   DAMJWP010000260 DAMJWP010000000
VERSION     DAMJWP010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4105
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.267"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            172..2271
                     /gene="nrdE"
                     /locus_tag="PC700_003316"
     CDS             172..2271
                     /gene="nrdE"
                     /locus_tag="PC700_003316"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDF2582585.1"
                     /translation="MNWIELNNQVIIKNEQGKYQLEKDKEALSSYIEEFIQPKLRKFN
                     NLEERLKYLIDEGYYLKEVINQYSIDVIKNIYDMIDKHKFEFQSYMSANKFYQNYALK
                     SNDGKEILETYNDKVLIVALTLGNGDKDLALNLADKIVKQEFQPATPTFLNAGRKRAG
                     EMVSCFLLSVEDSTEGISYAISSSNHLSKIGGGVALNLSKLRASGESIKDIEGAAGGV
                     VGVAKMLEQSFSYFNQMGARQGSGAVYLTVFHPDFELLMDTKKINADEKIRLATLSLG
                     AIIPDKFMELAEKNEVAYAFYPHTVYKKYGVSLDEIEMDEWYDKLVNDSDIRKKEINP
                     RQMLTKIAQMQQESGYPYVVYIDTANREHTLKDVGIIKMSNLCCEIFQYQTPSEIEGY
                     GGKNEWGQDISCNLGSLNIANVMDNKTIESTVETAIRALSFVADSTDIKPVPTVSNSN
                     SKSHSIGLGAMNLHGYLVRENILYTSDDAIDFSNVFFAMVRYYSIKASMKIAIERNQT
                     FEGFDKSEYVKGRNSKVLSKYYEQSYLPKSEKVRALFEGIYIPTKEDWTKLLDEVKEK
                     GIYNAYLMAVAPTQSISYVQNATSSIMPITEPVEVRTYGDSTTIYPMPFLTNDNMLYY
                     QSAYRMDMRKVIDLVATVQNHVDQGISTTLFVTDEKTTRDIARHYIYAYKKGLKSLYY
                     TRTKMTRDTHECLVCSV"
     gene            2284..3255
                     /gene="nrdF"
                     /locus_tag="PC700_003317"
     CDS             2284..3255
                     /gene="nrdF"
                     /locus_tag="PC700_003317"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDF2582586.1"
                     /translation="MTFNLHKIHNAVNWNREEDGFTQAFWEQNVKQFWLPEEISVSKD
                     IKVWNELSNKEKELYKKVLGGLTLLDTKQGNNGIPSMMSLTENLQRKAVLSFMGTMEE
                     IHAKSYSSIFMTLLSNLEIDELFEWIETEPTLQRKADLVLAQYENTTNQEGLYLSMVT
                     SVFLESFLFYSGFFYPLYLSGQGKMVASGEIISLILRDESLHGKYIGLLAQEIYDSFD
                     KMDKKMLEEKMYSILYSLMENEIEYTNVIYRESGLEKEVVNFLKYNANRALENLGFEK
                     LYTVDAINPIVLNGLSTETKTHDFFSTKGNGYQKGVYEELEDEDFII"
     gene            3331..3957
                     /locus_tag="PC700_003318"
     CDS             3331..3957
                     /locus_tag="PC700_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDF2582587.1"
                     /translation="MKYEWRKRDKELYLPKKNPVIIEIPEQKFIMLKGSGDPNSKEFI
                     EAIGVLYSLAYAIKTMPKKGIVPEGYFDYTVFPLEGIWDKGEKSKGLDVLIKEDLIYT
                     IMIRQPDFVTQDLLDKATDIVKKKKPHNLLEKVRFGSINEGLCVQMLHIGSYDNEPES
                     FDIMNEFCKKNKLIRKSYTHREIYISDARKVSPESLKTVLRFEVEREV"
BASE COUNT         1640 a          446 c          766 g         1253 t
ORIGIN      
        1 tataatagat aaagcactga ctatatgtag tgcttttgtt tttgaaaaat acttattgca
       61 tttctataat tatagattaa ttttgggatt tcaactgata agtaccaaaa gaaataataa
      121 gcaaattaag tgaaattatg gcacaagatt atgttaaaag gacggtaaat aatgaattgg
      181 atagagctta acaatcaagt gataattaaa aatgaacaag gtaagtacca attggaaaag
      241 gataaggaag ccttaagtag ttacatagaa gaatttatac aaccaaagtt gagaaaattt
      301 aataacctag aagaaagatt gaagtattta atagatgaag gttattattt aaaagaagta
      361 ataaatcagt atagcataga tgttattaaa aatatatatg atatgattga taaacataag
      421 tttgaatttc aatcatatat gagtgcaaat aagttttatc aaaattatgc attaaagagc
      481 aatgatggaa aggaaatatt agaaacatat aatgataaag tgttaattgt agctttgaca
      541 ctaggaaatg gcgataaaga tttagcttta aacttggcag ataaaatagt aaaacaagaa
      601 tttcaaccag ctacacctac ttttttaaat gctggaagaa agagggctgg agaaatggta
      661 tcatgttttt tactatctgt agaagatagt acagaaggta tatcatatgc tatatcttca
      721 tcaaatcact tatcaaagat tggtggagga gttgcactaa atttatctaa acttagagca
      781 tctggcgaat ctatcaaaga tatagaaggt gcagctggag gtgttgttgg agtagctaaa
      841 atgttagaac aatcatttag ttactttaat caaatgggag ctagacaagg ttctggtgcg
      901 gtgtacctta ctgtattcca tcctgatttt gaattgttga tggatactaa aaagataaat
      961 gcagatgaaa aaataagact tgctacatta tcattaggag ctataatacc agataaattt
     1021 atggagttgg cagaaaaaaa tgaagtagca tatgcatttt atcctcatac tgtgtataag
     1081 aaatatggag tttctcttga tgagatagaa atggatgagt ggtatgataa attggttaat
     1141 gatagtgata taagaaaaaa agaaataaat cctaggcaga tgttgacaaa aatagcacaa
     1201 atgcaacaag aaagtggata tccatatgta gtgtacatag atacagctaa tagagaacat
     1261 acattaaaag atgttggaat tattaaaatg tctaacttat gttgtgagat atttcaatat
     1321 caaacacctt cagaaataga ggggtatgga ggaaaaaatg aatggggaca agatataagt
     1381 tgtaacctag gttctttaaa catagccaat gtaatggata ataaaactat agaaagtaca
     1441 gttgaaacag caataagagc actgtcattt gtagcagata gcacagatat aaaaccagtg
     1501 ccaacggtaa gcaatagtaa cagcaagtct cattctatag gattaggagc tatgaattta
     1561 catggatact tagtaagaga aaatatttta tatacttcag atgatgctat agatttttct
     1621 aatgtatttt ttgcaatggt tagatactat tcaattaaag cttctatgaa aatagccata
     1681 gaaagaaatc aaacttttga aggatttgat aaatcagaat atgttaaggg aagaaatagc
     1741 aaggttttat caaaatacta tgaacaatct tatctaccta agtcagaaaa ggtaagagca
     1801 ttatttgaag ggatttatat accaacaaaa gaagattgga caaaattatt ggatgaagta
     1861 aaagaaaaag ggatatataa tgcatattta atggcagtag caccaactca aagcattagt
     1921 tatgttcaaa atgctacatc aagtataatg ccaattactg agccagtaga agtaagaaca
     1981 tatggagact ctactacaat atatccaatg ccatttttaa caaatgataa tatgttgtat
     2041 tatcaatcag catacaggat ggatatgaga aaggtcatag atttagtggc aacagttcaa
     2101 aaccatgttg accaaggtat atcaacaaca ctgtttgtta cagatgaaaa gacaactaga
     2161 gatatagcta gacattacat atatgcctat aaaaaaggtt tgaaaagttt atattacaca
     2221 agaacaaaga tgacaagaga tactcatgag tgtctagtgt gttcagtata gggagagaaa
     2281 tatatgacat ttaatttaca taaaatacat aatgcagtta actggaatag agaagaagat
     2341 ggatttacac aagctttttg ggaacaaaat gtaaaacaat tttggcttcc tgaagaaata
     2401 tctgtttcta aagacataaa ggtatggaat gaactaagta ataaagaaaa ggaattatat
     2461 aaaaaagtat tgggtggttt aacattactg gatacaaaac aggggaataa tggaatacca
     2521 tctatgatga gtttaacaga aaacctacaa agaaaagctg tcttgtcttt tatggggact
     2581 atggaagaaa tacatgcaaa aagttattct tctatattta tgactctact ttctaattta
     2641 gaaatagatg aattatttga atggatagaa acagaaccaa cattacaaag aaaggctgat
     2701 ttagttttag ctcaatacga aaatactaca aatcaagagg gattatacct atcaatggtt
     2761 acaagtgtat ttttagagag tttcttgttt tattcaggat tcttttatcc attgtatcta
     2821 tctggtcaag gaaagatggt agctagtgga gaaataatat cgcttattct tagagatgag
     2881 tctttacatg gtaagtatat aggattgcta gcacaagaaa tatacgattc atttgataaa
     2941 atggataaga agatgttaga agagaagatg tattctatac tttatagcct tatggagaat
     3001 gagatagagt atacaaatgt tatatacaga gaaagtgggc ttgaaaaaga ggttgtgaac
     3061 tttttaaaat acaatgcgaa tagagcttta gaaaatctag ggtttgagaa gctatatact
     3121 gtagatgcta taaatccaat tgtattaaat ggtcttagca ctgaaaccaa aacacatgat
     3181 ttcttttcaa ctaagggtaa tggataccaa aaaggagtgt atgaggaatt agaggatgaa
     3241 gattttataa tataatttat aattatgaca cactgttgtc acttttagcg tgataatatc
     3301 aatgtataaa atatatatgg aggtattttt atgaaatatg aatggagaaa aagagataaa
     3361 gagttatatt tacctaaaaa gaatcctgta ataattgaaa taccagaaca gaaatttatc
     3421 atgttaaaag ggagtggaga cccaaattct aaagaattta ttgaagcaat aggagtactt
     3481 tattctttgg catatgcaat aaaaaccatg cctaaaaaag gaattgtacc agaagggtat
     3541 tttgactata cggtttttcc tttagaaggt atatgggata agggagaaaa aagtaaaggc
     3601 ttagatgtac ttattaaaga agatttaatt tacactataa tgatacgtca gccagatttt
     3661 gttacacaag acctgttgga taaagctact gatatagtaa agaagaagaa acctcataat
     3721 cttcttgaaa aagttaggtt tgggtctatt aatgaagggc tatgtgttca aatgcttcat
     3781 ataggttcat atgataatga accagaaagt tttgacatta tgaacgaatt ttgcaaaaaa
     3841 aataaactta taagaaaatc ttacacacat cgtgaaattt atataagtga tgctagaaaa
     3901 gtatcgcctg aatcattgaa gacggtttta cgttttgaag tagaaaggga agtataaata
     3961 ttttgaataa tttataagag ttataaaaag tgaagtctgt tgagacttca ttttttgatg
     4021 caattttaaa attaaatttg atgtttcata atattttaaa aactttctac ttttattagt
     4081 gtgaataact gttgctatta aataa
//
LOCUS       DAMJWP010000261         4068 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.268, whole genome shotgun sequence.
ACCESSION   DAMJWP010000261 DAMJWP010000000
VERSION     DAMJWP010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4068
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.268"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            116..1519
                     /locus_tag="PC700_003319"
     CDS             116..1519
                     /locus_tag="PC700_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2582588.1"
                     /translation="MEKKKIGLWSLVFMNVSALYGIRWIAKSTASSFGLGLGAIPMWL
                     LFSFIYFVPGALICAELASTYQKGDGGLYDWVKEAFGEKYGFLVSWLNWTAKIFWYSS
                     FLTFLAVNVSYTIGNPALADNKMFVLILSLIIFWALSLIATKGISFTKIFTNTGALGS
                     TIPSAILIIFSFVAVFMLKKHDIASTYTIASMTPKLNMDAFVAISAIMFGLAGAETAA
                     NFITEIDKPEKNFPKAILISAGIVASLYVLGSIAITMIIPPDQITASKGVLDALSAVC
                     TSLGIGSWLVQLIAFGIAFSVLGAIVLYIASPIKMLFGSVKKGIFPDSLVEVNEHKIP
                     SKAVILQAIIVTIILLVTTLMPSVDAIYNVLVTMTALTALFPYVLLYASYIKLRKERP
                     DEIRPYTMAKSTSTCLGLAKMVLVVTVVGILLSAAPVMPTFAENVIYEIEMIGGGLLV
                     ILSGLWLWNRYEKKTAK"
     misc_binding    1610..1850
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1893..2591
                     /locus_tag="PC700_003320"
     CDS             1893..2591
                     /locus_tag="PC700_003320"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDF2582589.1"
                     /translation="MNVIGIIGAMDEEVSILVDLMDIRETIKKASLEFYKGILKGKNV
                     VLVKCGIGKVNSALCAQILISEFKVDAIVNTGVAGALNEKLDVNDIVISTDAIQYDVD
                     TTAFGDPKGVIPRMKTSVFKADERLIDAAYKSSVEEVKTHKVLKGRVVTGDKFINSKE
                     LKEELVNDFGGYCGEMEGGAIAHVCYLNNTPFVIIRAMSDKADGSADVTYDVFVHDAA
                     NNSKDIVLNMLKSI"
     gene            2614..3615
                     /gene="trpS"
                     /locus_tag="PC700_003321"
     CDS             2614..3615
                     /gene="trpS"
                     /locus_tag="PC700_003321"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDF2582590.1"
                     /translation="MSEKKVIFSGAQPSGKMTLGNYLGAIKNWTELQDNYDCYYSIVD
                     LHAITVPQDPKVLRANAIELLAQYIACGLDPEKNTIFIQSHVKEHVELMWVLNTMTYM
                     GELSRMTQFKDKSQKSEANLNAGLFTYPVLMAADILLYQTDLVPVGDDQKQHLELARD
                     LANRFNNRFSPTFVVPEGYYPKGGARVMSLQEPTKKMSKSDSNENAFILLADDSDSIK
                     RKIKRSVTDSLGVVKYNDEQPGIKNLIDIYSNLSKKSVEEIVNMYEGKGYGIFKEDVA
                     EVVSEALRPIREKYVDLLNNKDYLEKIYSMGAEKAEKQARKTLRKVYKKVGFIERRY"
BASE COUNT         1526 a          454 c          734 g         1354 t
ORIGIN      
        1 ctgtttttga atatgcaaat attttgaaaa atatcagttt aataataatg aatctatttt
       61 attaattttg aaattaaatt taaaataatt aatagagagg ggaaatgaaa ttaggatgga
      121 aaagaaaaaa ataggcctat ggagtttggt atttatgaat gtatcagctt tatatggtat
      181 aagatggata gctaagtcta cagcatcaag ctttggttta ggattaggtg caatacctat
      241 gtggttatta ttctcattta tatactttgt accaggagct ttaatttgcg ctgaattagc
      301 ttctacatat caaaagggtg atggaggtct ttacgattgg gttaaggaag cctttggaga
      361 aaagtatgga tttttagttt cttggttaaa ctggacagct aaaatatttt ggtattcttc
      421 atttttaact ttcttagcag taaatgtttc ttatacaata ggaaatccag ctcttgctga
      481 taataagatg tttgtactta tattatcttt aataatattc tgggcattat ctcttatagc
      541 aactaaagga ataagtttta caaaaatatt tactaataca ggtgcattag gttcaacaat
      601 accaagtgct atacttataa tattttcatt tgtagcagta tttatgttga aaaaacatga
      661 tatagcttct acatatacaa tagcttcaat gactccaaaa cttaatatgg atgcatttgt
      721 tgctatatca gctataatgt ttggtttagc aggagcagag actgctgcta acttcataac
      781 agaaatagat aagcctgaaa agaacttccc taaggcaata ttaatatcag ctggaatagt
      841 agcttcatta tatgtattag gttcaattgc aataacaatg ataataccac cagaccaaat
      901 tactgcttct aaaggtgtat tagatgcact ttctgctgtt tgtacgtctt taggaattgg
      961 ttcttggttg gttcaattaa tagcttttgg tatagctttc tcagtattag gagcaatagt
     1021 tctttatata gcatcaccaa taaaaatgtt atttggtagt gttaaaaaag gaatatttcc
     1081 agatagtttg gtagaggtaa atgaacacaa gataccttct aaagcagtta tacttcaagc
     1141 aataatagtt actataatac tattggtgac tacattaatg ccttctgttg atgctatata
     1201 caatgttctt gtaactatga cagcattaac tgcattattc ccatatgtat tattatatgc
     1261 ttcttatatt aaactaagaa aagagagacc agatgaaatt agaccttata ctatggcaaa
     1321 atcaacaagt acttgcctag gtttagcaaa aatggtttta gttgtaactg tagttggtat
     1381 attattatct gcagcaccag ttatgccgac atttgctgaa aatgttatat atgagataga
     1441 gatgattggt ggaggtcttc tagttatatt atctggtcta tggttatgga atagatatga
     1501 aaagaaaaca gctaaataaa atgtatttta aaaatttctt ttggatatta taatatatag
     1561 ttgacaattt atagtgtaag gaatattata aaataaagaa attaataaat taaatcctga
     1621 gataagaaat agtaaataaa tatatttttg cacagagaat taaggatgct gagaacttaa
     1681 taaaaagtat ttattgaatg ggtctttgag gagtaaaatg aaatgtaatt taagattaca
     1741 gagtatttga tatcgtgtat gcacgttata gcatatatta agatatatat attcagtttt
     1801 gagtgtatat aaatttaggt ggtaccacgg aaaatatatc cgtcctattg atttaggacg
     1861 gattttttaa tatagaaaat ttaggaggaa aaatgaacgt aataggaatt ataggtgcaa
     1921 tggatgaaga agttagtatt ttagttgatt taatggatat tagagagact ataaaaaaag
     1981 ctagcttaga attttataag ggaattttga aaggtaagaa tgtagtgtta gtaaagtgtg
     2041 gtataggtaa ggttaactct gctctttgtg cccagatact aataagcgaa tttaaggttg
     2101 atgctattgt taatacaggt gttgcaggag ctttaaatga aaaacttgat gttaatgata
     2161 tagtaatatc aactgatgct atacagtatg atgtagatac tacagcattt ggagacccta
     2221 agggagttat accaagaatg aagacttctg tatttaaagc agatgaaaga cttatagatg
     2281 ctgcttacaa atcttctgtg gaagaagtaa agactcataa agtattaaaa ggaagagtag
     2341 ttacaggaga taaatttata aattctaaag aattaaaaga agaattagta aatgattttg
     2401 gtggatattg tggagaaatg gaaggtggag ctatagctca tgtatgctat ttaaataata
     2461 ctccattcgt aataataaga gcaatgtctg ataaggcaga tggaagtgct gatgttactt
     2521 atgatgtatt tgtacatgat gctgccaata actctaaaga catagtttta aatatgttaa
     2581 aatcaatata aatatataag gaggtacaaa aaaatgagtg aaaaaaaagt tatatttagt
     2641 ggagcacagc catcaggcaa aatgacttta ggaaattatt taggagctat aaaaaactgg
     2701 actgaacttc aagataatta tgattgctat tatagtatag ttgatttaca tgcaataaca
     2761 gtacctcagg accctaaagt tttaagagcg aatgctatag aattacttgc tcaatatata
     2821 gcttgtggat tagacccaga aaaaaatact atatttatac aatcacatgt caaagagcat
     2881 gttgaactta tgtgggtatt gaatactatg acttatatgg gtgaattaag tagaatgact
     2941 caattcaaag ataaatcaca aaagagtgaa gcaaatctaa atgcaggttt attcacatat
     3001 ccagttctta tggccgctga catattgttg tatcaaactg acctagttcc agttggagat
     3061 gaccaaaaac aacatttaga attagctcgt gatttagcaa ataggtttaa taatagattt
     3121 tcacctactt ttgtagttcc agaaggatac tacccaaaag gtggagcaag agtgatgagt
     3181 ttgcaagagc caactaaaaa aatgagtaaa tccgattcaa atgaaaatgc atttatacta
     3241 ttagcagatg attcagattc tataaagaga aagataaaaa gaagtgttac tgattcttta
     3301 ggtgttgtaa aatacaatga tgaacaacca ggtattaaaa atctaattga tatatattct
     3361 aatttaagta aaaagagtgt agaagagatt gtaaacatgt atgaaggaaa gggttatggc
     3421 atatttaaag aagatgtggc tgaagtagta tcagaagctt taagacctat aagagaaaaa
     3481 tatgttgacc ttcttaataa taaagattat ttagaaaaaa tatatagtat gggagcagaa
     3541 aaagccgaaa aacaagctag aaaaactctt agaaaagttt ataaaaaagt tggatttata
     3601 gagagaagat actaattatt agctatcttt acttgataaa taaattttaa agttattaaa
     3661 aaggtatatg atatgaaaga gagagtctca aagtgaactt ttgttcatga gacactcttt
     3721 tttgtatgaa ataaaatata ttttaatcac ttcaacaatg aaaaagagga ttatctctat
     3781 tttgagacag catcttttga taataaaaaa agatgagctt ttgctcatct caaaggggtt
     3841 agattattct tactttgggg aaagtagaat aaaggtaaaa gttaactgat gtcaactttg
     3901 tcttttttgc atatcactac tgagcataaa aaaatcagaa aaccatagcc ttctgaaaca
     3961 acttctaaca tacattgtat tttataccca atatgtaatt tatgagttaa ttttaactct
     4021 tataatggaa attgtcaata atatatcaaa aaaatatttt ttaaattt
//
LOCUS       DAMJWP010000262         3992 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.269, whole genome shotgun sequence.
ACCESSION   DAMJWP010000262 DAMJWP010000000
VERSION     DAMJWP010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3992
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.269"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(86..1003)
                     /locus_tag="PC700_003322"
     CDS             complement(86..1003)
                     /locus_tag="PC700_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HDF2582591.1"
                     /translation="MVNMNIIEIRSDKIYKKIMDAPINKKEDIYRYELMKPFEFKWKC
                     MNVPIVARQKGGYDVIIASEMLGVLSPKDIDEKQKKNINVLSADKIWGTCKETIENSI
                     NAFIKEGYDLNIKDYKYSILLANPNSPYTILSDGYWGDGGIPGYIFLSLVPNEYTINR
                     LPVLIAHECNHNIRFQFIEWNNNITLEEMMINEGLAENFATWMFGEEMLGPWVSRTDI
                     ETLNTYIKPIIKSALKETGFQNITSYLYGDDIAKMQGYFPVGLPYCAGYACGYYMIKY
                     YLEKTNKSIIEATLLPYSEIIETVKEFWE"
     gene            1265..1642
                     /locus_tag="PC700_003323"
     CDS             1265..1642
                     /locus_tag="PC700_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2582592.1"
                     /translation="MLTPYLIFNGTCEKAFNFYAEAFGGGKTIFARLDSNPNNPIMHA
                     SVTFTKYEGCIMGADTDKPVVISGMAICVVLPSREAIEEISVKLAEGGTLVQEFLPHP
                     PPHQNDGAAEVLDRYGYTWYLST"
     gene            complement(1798..2247)
                     /locus_tag="PC700_003324"
     CDS             complement(1798..2247)
                     /locus_tag="PC700_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2582593.1"
                     /translation="MKSGIILEMKQINSTEYDEMLQVWESSVRATHDFLTEKDIESLI
                     PLVEIGLKEVENIVCIKDNDIIKGFIGIDKDKIEMLFIEDKYRGNGIGKKLIKYAIDK
                     YNVKYVDVNEQNKKAVGFYIHLGFKVFDRSEIDGQGNPFPILHMKLI"
     gene            complement(2425..2829)
                     /locus_tag="PC700_003325"
     CDS             complement(2425..2829)
                     /locus_tag="PC700_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HDF2582594.1"
                     /translation="MSYQNYKCERDYVYDDYKYNKCNKCYNDYEEMTHVHEYSESVKL
                     AEECEDRHNHRAAGVTGEAIPINGGTNHVHKINDNVDFLDHFHKICVTTGPAIRIPGT
                     DKHIHLICGETTVNDGHCHKFLFTTQIEAPLV"
     gene            complement(2942..3808)
                     /locus_tag="PC700_003326"
     CDS             complement(2942..3808)
                     /locus_tag="PC700_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDF2582595.1"
                     /translation="MIDIHKNIYDNKLFEELKIDCKKCFGLCCVALYFSASDGFPIDK
                     ESGKPCINLQPDFKCSVHNSLMKRGFKGCTAYDCFGSGQKVAQVTYKGIDWMQSPELT
                     NQMSEVFLIMRQLHEMLWYLKEASVLNISDTIKSKIDLIIEETEKITNMSPEQIINLD
                     IISHRTKVNLLLSQASESVMGKVKSFIKTSTLKNMKKLSKNIDLIGADLRKINLIGAD
                     LRGRFLIAANLRSTDLSGANLIGADLRDCDIRGANLENSIFLTQLQVNTAKGDSSTKL
                     PASLIRPKYWEK"
BASE COUNT         1302 a          676 c          437 g         1577 t
ORIGIN      
        1 atttcttata aattattgtc aatttatttt ttcttaaaaa taaaataatt gcataattta
       61 tttcaaatta tgcaatttat aaaatttatt cccaaaactc ttttactgtt tcgattatct
      121 cactataagg caataaagtc gcttcgatta ttgatttatt tgtcttttct aaataatact
      181 taatcatata atatccacaa gcatatcctg cacaataagg caaccctact ggaaaatatc
      241 cttgcatttt agctatatca tcaccataaa gataagatgt tatattttga aatccagttt
      301 cttttaaagc actttttatt attggcttta tataagtatt taatgtttcg atatctgttc
      361 tactgaccca aggtcctaac atttcctctc caaacatcca tgttgcaaaa ttttctgcaa
      421 gaccttcatt tatcatcatt tcttctaatg ttatattatt attccactct ataaactgaa
      481 atctaatatt gtgattacat tcatgtgcta ttaatactgg taatctattg atagtatatt
      541 cattaggaac caatgataga aatatatatc caggaatccc accatcaccc cagtatccat
      601 cacttaatat tgtataagga ctatttggat tcgccaataa tattgaatat ttatagtcct
      661 taatgtttaa atcataccct tcttttataa aagcatttat agagttttct atggtttctt
      721 tgcaagtgcc ccaaatttta tcagcagata acacatttat attctttttt tgcttttcat
      781 caatatcctt aggcgataaa acccctaaca tttcacttgc tataattaca tcatatccac
      841 ctttctgtct agcaactatt ggaacattca tacacttcca cttaaattca aaaggcttca
      901 tcaattcata tctgtatata tcttcttttt tgtttattgg tgcatccatt atcttcttgt
      961 atattttatc tgagcgaatt tctataatat tcatatttac catctccttt aagacaaagt
     1021 atatacaatt acgtagcgtc aatgtcaata tacatttttt ataatgtcac cttaatcttt
     1081 ttattatatt ttatcctgta aaccttccgt acatataaca tttttttaaa ttaataaatt
     1141 tgtataatat aataataaaa aattatatac ttcaaggttt ccctatgata caatttacta
     1201 aatcataatt tagattatca ggtgcaacta taaattattc tatatcaagg aggatatatt
     1261 ggtcatgcta actccatatt taatatttaa tggtacttgt gaaaaagcat ttaattttta
     1321 tgctgaggct ttcggaggag gaaaaactat atttgcgcga ttagacagca atccaaacaa
     1381 tcctattatg cacgcaagtg ttactttcac aaaatacgaa ggttgtataa tgggtgcgga
     1441 tacagacaag cctgttgtaa tttctggcat ggcgatttgt gttgttctac catctcgaga
     1501 agcgatagaa gaaatatctg taaaacttgc cgagggtggt acacttgtac aagaattttt
     1561 accacaccca ccaccacatc aaaatgatgg cgctgctgaa gtacttgata ggtatgggta
     1621 tacttggtat ttaagtacat agtaaaaata aaaaaactac atctaataat cataatatag
     1681 aacagataaa tttcaattta atagtgtagt aaaaaagtcg tgtcctaaga ctgtcacgac
     1741 ttttatttgt ataaaaattt aagtaaatat ttttgcgaat aagtgagaag tataacttta
     1801 aattaatttc atatgtagta ttggaaatgg gttaccttgt ccatctattt cagacctatc
     1861 aaacacttta aatcctaagt gaatataaaa tcctacagct tttttattct gctcatttac
     1921 atcaacatat tttacattgt atttatctat agcatatttt attaattttt taccaatacc
     1981 atttcctcta tatttatctt ctataaagag catctcaatt ttatctttat ctattcctat
     2041 aaagcccttt attatatcat tatcttttat acaaactata ttttctactt ctttaagtcc
     2101 aatttcaact aatggtatca aagattcaat atctttttct gttaaaaaat catgcgttgc
     2161 tcttactgat gattcccaga cttgcaacat ttcgtcatat tctgttgaat ttatttgttt
     2221 catttcaaga attattccag atttcataaa atttcctccc tatatcactt ttaattgtat
     2281 aacacttttt tatacaaaac ttagaaagga ctacgaaggt taagtatcat agtcctaatt
     2341 aagtttaaat attatctatt atatattttt aacatttatt atatactata atctagcaaa
     2401 tttactacaa taatccatat cagtttatac taaaggtgct tctatttgag tagtaaaaag
     2461 gaatttatga caatgaccat cattaacagt agtttctcca catattaagt gaatgtgttt
     2521 atcagttcca ggtattctaa ttgctggccc tgttgtaaca catatcttat ggaaatggtc
     2581 aaggaaatct acattatcat ttattttatg aacatgattt gtacctccat ttattggtat
     2641 agcttcacca gtaacccctg ctgcacggtg gttatgtcta tcctcacatt cttctgctaa
     2701 cttaacactt tctgaatatt catgaacatg tgtcatttcc tcataatcat tatagcactt
     2761 gttgcactta ttgtatttat aatcatcata cacataatct ctttcacatt tataattttg
     2821 ataactcatt tttatatctc tccctatatt atattaagaa attcttgtaa agaatctcta
     2881 atgtataata tgaattaaag tcaaaaaagt tcatattttg taaaattatg tattaattta
     2941 attatttctc ccagtattta gggcgtatta gtgaagctgg taacttcgta ctagaatctc
     3001 cttttgcagt atttacttgc aattgtgtta aaaatatact attttcaaga ttagcacctc
     3061 taatatcaca atctcttaag tctgctccta ttaaatttgc cccactcaaa tctgtacttc
     3121 ttaaatttgc tgctattaga aatctacctc ttaaatctgc tcctattaga tttatttttc
     3181 taaggtctgc tcctattaaa tctatatttt tacttaattt tttcatattt ttaagagtag
     3241 aagtcttgat aaatgattta acctttccca tcacagactc actcgcttgt gataataata
     3301 aattaacctt tgttctatgt gatatgatat ccaaattaat tatctgttct gggctcatat
     3361 tagttatctt ttcagtttct tcaattatca aatcaatttt tgatttgatt gtatctgata
     3421 tatttaatac agatgcttct ttaagatacc ataacatttc atgtaattgt ctcataatta
     3481 aaaacacttc agacatttga tttgttaatt ctggtgattg catccagtct atccccttat
     3541 atgttacttg tgctactttt tgacctgaac caaaacaatc atatgcagta caccctttaa
     3601 aacctcgctt cataagacta ttatgtacag aacatttaaa atctggttgt aaattaatac
     3661 agggttttcc tgattcctta tcgataggaa atccatctga agcagaaaaa taaagagcta
     3721 cacaacaaag tccaaaacac ttcttacaat caatctttag ctcctcaaat aacttattat
     3781 catatatatt tttatgtata tctatcatat tttttctcct cttaagcttt atttttaata
     3841 ttatattatc tataactaaa tgataatcaa gtcccttatt taacagttct tattcttaat
     3901 tataatttaa ggtattattt attccaatag catacataag tttctttata cttgtaacaa
     3961 tacctttata ctaagactta ttttaaatgt ag
//
LOCUS       DAMJWP010000263         3979 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.270, whole genome shotgun sequence.
ACCESSION   DAMJWP010000263 DAMJWP010000000
VERSION     DAMJWP010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3979
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.270"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            1063..1815
                     /locus_tag="PC700_003327"
     CDS             1063..1815
                     /locus_tag="PC700_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2582596.1"
                     /translation="MLKVNQLCKSYKTGNKTYPVLKNISFEINKGEFVAIMGSSGSGK
                     TTLLNCISCFIPIDNGEIILGNNNIRNLKEKELSEVRNQQLGFVFQDFMLFDGLSVFE
                     NICVPQIIKGSQVLQMENRAINLCELFGIEHIRKKYPAEISGGEKQRTAVARALMNNP
                     DLILADEPTGNLDSKSCRTVIESFLKAKQELDATILMVTHDSFAASFCDRVIVLKDGI
                     IYKEVVKSNSRHKFLDELLEVLKEMGGAQDDN"
     gene            1805..3916
                     /locus_tag="PC700_003328"
     CDS             1805..3916
                     /locus_tag="PC700_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2582597.1"
                     /translation="MTINKLYSKLRAYNWKNYTTLFFCIILSVVLVTSYALIYFSPTI
                     QNILPTGGDSRKQATMIFSVAILGCAVFTTYASSLFLKFKSKEIGIFMALGTDKKKLK
                     KLLFSEILLISIVCCFLGLILSIPVSFGIWKIFQLVIIDTREMTYHFGTQGFIYGFIF
                     SVFVTLCIFILSVKFINRTNIIDILNDQRISEPIQDVKDWYGILGIIFIILGIFLGYI
                     VPSLTINKLNYRLPSVWSFTYLLSLIGIYMIMTYIVVHAKRGKHPEKYYKNIISTSMM
                     RFMGRQTVKNMCVISFLIAGALFAVFYVPNMVAGIYDTLKNNPIDYTFTYNQRDKQVS
                     KKDIEQLADKHNIKIQNYMEIPSIELIVDGVESIYHKDGTIERVYMKKSSYDMFFNSK
                     DFSKLIDKPITIKNGEYLAVVNKGNENQDYKYTKFISSPVTEKEIPFRYAGKIIFNGS
                     FFQNRSMNAYVLNDEDYKSFTKDLSIKNYNNSILFNVDKDTYAFANDLKKEIIKRTSK
                     EMGMFSGYDEYVKKRYESTGRKYFMDEEYPSGEGHLKLDPNNNQLYLNWKYYPNFKIL
                     QSQDMIKNMAVFLMLFIYVAIICFTAVGIIAYTRGVTIAINHKQVFMDLNRLGANNDY
                     IKFCIRNQLKKVFSYPLIAGSFLIYLFNFMIMLNNDGRIVNSEVNALLINLGFIGICG
                     VYMYLIYLLTYKKFKKIIGIL"
BASE COUNT         1468 a          573 c          502 g         1436 t
ORIGIN      
        1 tttattgcag tgttttgtta ttacagtatt tctattatat tgcaaactct tatttattat
       61 ggtgttttgt tattgcaaca tttctattat attataaagt cttatttatt acagttcttt
      121 gttatactac agacttttat tattacagca tttctattac aatctcttat ttattacagt
      181 tctttgctat actacagacc tttattattg caacatttct attacattgc aaagtcttat
      241 ttattacagt tctttattat actacagact tttattatta caacatttct attatattac
      301 aatctcttat ttattacagt tctttattat actacagact tttattatta cagcatttct
      361 attacattgc aaagtcttat ttattacagt tctttattat actacaaacc tttattatta
      421 caacatttta ttattttaac gtctttacta taatatagta atattttaat atctcaaaat
      481 catatcaatc acttttcagc atagtctaaa atacttccac tttctaaaat tatctatcat
      541 acctatacta ataatacttt ttaacctttt tattatacat ttcaattgtt tttaacgacc
      601 tatttatttc caaaactcca atatattttc tcaattacat tgtgttaata acccctaaat
      661 cctttcaaat tatttacaat acattccatt aaataatctt actgctctat aattcttttt
      721 ttctttttaa ccctactata cattatttac atcatttttt tcaatacaac attcgttatt
      781 ctataacctt tttactttta ctatatacct tttttctaac tacaccttca atgttccaca
      841 tcatattact ttagcacaac aattaatacc taaaaataac cctctaaatc ctccaataat
      901 ataatcaatt taactataat aataacctct cacactatcc aaacaatata ttttcaaaca
      961 ttacaaaaaa acatccaact attacaatat tgctcatatc cctcctcccc tttcaaatgc
     1021 tatcattgac ttacaagata aatgaaagtg aggatgatat atatgcttaa agtaaaccaa
     1081 ttatgtaaat catataaaac aggcaataaa acatatcctg tattaaaaaa tatttctttt
     1141 gaaatcaaca aaggagagtt tgtcgcaatc atgggttcat caggaagtgg aaaaactact
     1201 cttttaaatt gtatttcatg ttttatccca attgataatg gggaaataat tcttggaaat
     1261 aataatataa gaaatttaaa agaaaaggaa ctttcagaag ttagaaacca acaactagga
     1321 tttgtattcc aagatttcat gctatttgat ggtcttagtg tctttgaaaa tatatgtgtt
     1381 ccacaaatta tcaaaggtag tcaagtctta caaatggaaa accgtgcaat taatctatgc
     1441 gagctatttg gtattgaaca tatcagaaaa aaataccctg ccgaaatatc tggtggagaa
     1501 aaacagcgta cagcagtagc aagagcactc atgaacaacc cagatttaat attagcagat
     1561 gaaccaactg gtaaccttga tagtaagtct tgtcgtacag tcattgaatc atttttaaaa
     1621 gcaaagcaag aattagatgc tactatactc atggttacac acgatagctt tgcagcaagc
     1681 ttctgcgacc gagttattgt gcttaaagat ggaataattt ataaagaagt tgtaaaatca
     1741 aattcaagac ataaattcct agatgaatta ctagaagttt taaaagaaat gggaggcgct
     1801 caagatgaca attaataaat tatacagcaa acttcgagcc tataattgga agaactatac
     1861 aactttattc ttttgtataa tactttctgt ggtattagta acttcatatg ctctaattta
     1921 ttttagccca acaatacaaa acatacttcc aactggtgga gatagcagaa aacaggctac
     1981 aatgatattc tcagttgcta tattaggatg cgctgtcttt actacatacg cttcttccct
     2041 ctttttaaaa tttaaaagca aggaaattgg tattttcatg gctctaggaa cagataagaa
     2101 gaaattaaaa aaacttctat tttcagagat attgttaatt tcaatagtat gctgtttttt
     2161 aggtcttata ttaagtattc ctgtatcttt tggtatatgg aaaatatttc aattagtgat
     2221 tattgatacc agagaaatga catatcattt tggaactcaa ggattcatat atggatttat
     2281 atttagtgta tttgtgacat tatgcatatt tatactttct gtaaaattta ttaatcgtac
     2341 aaatataatt gatatattaa atgaccagag aatatctgaa ccaatacagg atgtaaagga
     2401 ctggtatggt atattaggta taatttttat tatactaggt atttttctag gatatatcgt
     2461 accttcacta actataaaca aattaaatta tagactacca tctgtatgga gctttactta
     2521 tctactctct cttattggaa tatatatgat tatgacatac atagttgtac atgctaaaag
     2581 aggaaaacat ccagaaaaat actataaaaa catcatatca actagcatga tgagatttat
     2641 gggaagacaa acagtaaaaa acatgtgtgt tatttcattt ttgattgcag gagcattatt
     2701 tgctgtattc tatgttccta atatggttgc aggtatctat gatactctta aaaataaccc
     2761 tatagattat acttttacct acaatcaaag agataaacag gtatcaaaaa aagatattga
     2821 acaattagca gataaacaca atataaaaat tcaaaactat atggaaattc cttctataga
     2881 attaattgtt gatggtgtag aatcaatata tcataaagat ggaactatcg aaagggtata
     2941 tatgaaaaaa agctcctatg atatgttctt taattctaaa gatttttcca agctaattga
     3001 caaaccaata actataaaaa atggtgaata tcttgctgtt gtaaataaag gaaatgaaaa
     3061 tcaagattat aaatatacca aatttatatc aagcccagtc acagaaaaag aaatcccctt
     3121 tagatatgct ggaaaaataa tatttaatgg ttcttttttt caaaatagaa gtatgaacgc
     3181 ctatgtcctt aatgatgagg attataaatc atttactaaa gatttatcca taaaaaatta
     3241 caataattct atactattta atgtagataa agatacctat gcttttgcca atgacttaaa
     3301 gaaagaaatt ataaaaagaa catctaagga aatgggcatg ttcagtggtt atgatgaata
     3361 cgtaaaaaaa cgttatgaat caactggaag aaaatatttt atggatgaag aatatccaag
     3421 tggtgaaggt catttaaaac tagacccaaa caacaatcag ttgtatctta attggaaata
     3481 ctatcctaac tttaaaattt tacaaagcca agatatgata aaaaacatgg cagtcttctt
     3541 aatgttattc atatatgtgg caatcatatg ttttacagca gtaggaatca ttgcatatac
     3601 aaggggtgtt actatagcta taaatcacaa gcaggtcttt atggatttaa atcgattagg
     3661 agcaaacaat gattatataa agttctgtat tagaaatcaa ttaaagaaag tattttcata
     3721 ccctcttatt gcaggaagtt ttttaattta tctgtttaat tttatgatta tgttaaataa
     3781 tgatggtaga atcgtcaata gtgaggtcaa tgctcttttg ataaacttag gttttatagg
     3841 gatttgtgga gtttatatgt accttatata tctattaact tataagaaat tcaaaaaaat
     3901 tattggtatt ttataataag gaaggttgtt agatttttaa agtaataaaa tcattacttt
     3961 ttataataag aaaagcatt
//
LOCUS       DAMJWP010000264         3963 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.271, whole genome shotgun sequence.
ACCESSION   DAMJWP010000264 DAMJWP010000000
VERSION     DAMJWP010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3963
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.271"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            1..391
                     /locus_tag="PC700_003329"
                     /pseudo
     CDS             1..391
                     /locus_tag="PC700_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816433.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
     gene            complement(751..1905)
                     /gene="nagA"
                     /locus_tag="PC700_003330"
     CDS             complement(751..1905)
                     /gene="nagA"
                     /locus_tag="PC700_003330"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDF2582598.1"
                     /translation="MKSFIYADKFFMKHGIKEKGYLSIINGKFGEFQQERPNITDNVI
                     DFSGKYIAPGLVDTHIHGLLGADVMDNTFEAINTISKGLLKYGVTSFLPTTLTDSVDT
                     LSDSVENISNSFNRVEGAKIQGIYLEGPFFTEKYKGAQNEKYFKDPDINILKLWQRKS
                     KNLIRKIAIAPERKGSIELTRYATKNNISVSLGHSSATFEQVKQVVNSGAKVFVHTYN
                     GMSPLNHREPGMVGAAMVLKDTYAELICDGHHVSPIAVKIMMDAKSSNNIALITDCMR
                     AGAMEDGKYTLGKFSVNVKNSVARLNSGSLAGSVLTMDKAVRNIVDWNIATLEDAIKM
                     ATYVPALSCNIDNVCGSIHQGMDADFIVTDKNFNIYETYISGICMYKVNK"
     gene            complement(1988..2713)
                     /locus_tag="PC700_003331"
     CDS             complement(1988..2713)
                     /locus_tag="PC700_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2582599.1"
                     /translation="MIKRNDKRPIYDQLVEILRTKIENEMEPNDRMLSERKICDEYGV
                     SRTTVRLAMAELEHMGYIYKRHGKGTFVAALSQNSQNLMESYSFTDYMRIQGKKPGTK
                     VLSFEVVESTKYFSEKLGITPGEKMIKIMRLRLADDLPMMLERTYLPMKEFIGLTKEK
                     IEQKPLYEIFREDYGEIIKVADEEFSAGIISDKEAKLLEVPGDSPCLKLLRTTYNDDN
                     IVIEFTLSVARSDKFVYRVRHVR"
     gene            2982..3911
                     /gene="pfkB"
                     /locus_tag="PC700_003332"
     CDS             2982..3911
                     /gene="pfkB"
                     /locus_tag="PC700_003332"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDF2582600.1"
                     /translation="MITVITFNPSIDRMYKVNNINIGEVQRVVSKNATAGGKGLNVAK
                     VCKILKENPLAMGFLGGFNGEFIKNELRKLDIQNKFTKIEQETRNCLNIIEDNKVSTE
                     FLEKGPIVEKSDLERFENDIEEVLKNTKILVASGSYCENMPIDYYEKIGNICRANNIK
                     FILDTSGEALKIALKSKPYLIKPNIDEIRHLLGINIESREEIILSGKKLIEMGAENVC
                     ISLGKDGMVYLNEYNVYDVKVPVVKCINTVGSGDSTVAGFSVGILRGYKIENLLKFAN
                     ACGISNALNMETGFVDLGEIDKYQDLVEVKKLS"
BASE COUNT         1444 a          550 c          562 g         1407 t
ORIGIN      
        1 tgcaaaagat taaaagaaag tatatgctgc aattacagag gaagtagcat taagtgagct
       61 atcgctctta gaggaaaaat ggggtaatca atatgcgata gcattaagaa gttggcgaag
      121 caattgaaat gagctagcaa catactttaa atatcctcaa gagataagaa cattgatata
      181 tactacaaat tcaatggaaa gttacaatag attattaaga aaagttacta aatcgaagtc
      241 tatttttcct ttagatgatt ctttgcacaa atcattatac ttagcaacta tggatatttc
      301 tgaaagatgg actcaaaaga ttagaaattg gactcaaatc ttagctcatt taagtattta
      361 ttttgaagaa agaattttaa ctctaaatta ataaagaaat atttcaaaaa aattaaggaa
      421 ttttcttaaa ggggtaataa tttttacaaa aatggaaaaa atatcaaaat aaggctatga
      481 tttttaaagc catagcctta ccaataaatg tttaattatc actaaaaaat aaatttttat
      541 tcaaagaata cacataaata tttccagatt cttaatttta taaacttata ttgtaaaata
      601 aagaacctta ataaaaggag tgtctcaaaa ttaacttttt agtttatgag gcactctttt
      661 tgtatgaaat caaatgtatt ttcatcattt taacaatgaa aaagagactt atctctattt
      721 ttaggaagcc tccataactt tatattgaat ctatttatta actttgtaca tacatatacc
      781 acttatataa gtttcgtata tattaaagtt cttatctgtt actataaagt ctgcatccat
      841 accttgatgt atggagccac agacattatc aatattacaa cttaaagcag gaacatatgt
      901 agccattttt attgcatctt ctaaagtggc tatgttccaa tcaactatat ttctcacagc
      961 cttgtccata gttaatacac ttccagctaa actcccacta tttaatcttg caacactatt
     1021 ttttacattt acactaaact taccaagagt atattttcca tcctccatag caccagccct
     1081 catacaatct gtaattaaag ctatgttatt actagacttc gcatccatca ttattttaac
     1141 agctattggg cttacatgat gaccatcaca aattaattca gcataagtat cttttagtac
     1201 catagcagcc ccaaccattc caggttctct atgatttaaa gggctcatcc cattatatgt
     1261 atgtacaaac actttagcac ctgaattaac gacttgttta acttgttcaa atgtagctga
     1321 actatggcca agagaaacag agatattatt tttagttgca tatcttgtta attctattga
     1381 acctttacgt tctggagcaa ttgctatttt tcttattaaa tttttcgatt ttctttgcca
     1441 taattttaat atattgatgt cagggtcttt aaaatatttt tcattttgag cgcccttata
     1501 tttttctgta aaaaatggac cttctaaata aatcccttgt atttttgcac cctcaactcg
     1561 attgaagcta ttgcttatat tttctacaga atcacttagt gtatctacag aatcagttaa
     1621 agttgtaggt aggaaagatg ttactccata ttttaataag ccttttgaaa ttgtgtttat
     1681 tgcttcaaat gtattgtcca tgacatctgc acctaaaaga ccgtgaatgt gagtatcaac
     1741 taagccagga gcaatatatt tacctgaaaa atctatgaca ttatcagtaa tattaggtct
     1801 ttcttgttga aattcaccga atttgccatt aattatacta aggtaacctt tttctttgat
     1861 tccatgtttc ataaaaaatt tatctgcata aataaaactt ttcaaaataa cctccttata
     1921 attatactat cattgtggca ttgacgttta taatatcatt ctattttctt gaaagctatt
     1981 taattcacta tcttacatgt cttactctat atacaaattt atcgcttcta gcaacactca
     2041 aagtaaattc aataactata ttgtcatcat tatatgtggt acgaagtaat ttcagacaag
     2101 gtgaatctcc tggtacttcc aataacttag cttccttatc agagattatt ccagctgaaa
     2161 attcttcatc agcaacttta ataatctcgc cataatcttc tctaaatatt tcatataaag
     2221 gtttttgttc gattttttct tttgtcaatc ctataaattc tttcatagga agatatgttc
     2281 tttcaagcat cataggaagg tcatcagcaa gtcttaaacg cattattttt atcatttttt
     2341 caccaggagt tatacctaat ttttcagaga aatactttgt actttctact acctcaaatg
     2401 ataatacctt tgtacctggc ttttttcctt gtattctcat ataatctgta aagctataac
     2461 tttccattaa gttttgagaa ttttgactta gtgcagcaac aaaagttcct tttccatgtc
     2521 ttttgtatat atatcccata tgttccaact cagccatagc tagtctaact gtggttctac
     2581 ttactccata ttcatcacaa atttttcttt cagaaagcat tctatcgttt ggttccatct
     2641 cattttcaat cttagttctt aatatctcaa caagctggtc atatattggt cttttatcat
     2701 ttctttttat cataaaaaca tagtcctttc taagtggtta ttaccacaat ttgattatag
     2761 aatatagaat tatttatgtc aatgaataaa gtttaatcaa aattcaataa gtaaggctat
     2821 taatttatta taaaattatg gtatattgac aaaacaagta aaataattta tattcaaatt
     2881 ggtaataacc actttgggta tattatagat gaggtatttt ttattttaca gattaattta
     2941 ttgtttagaa aagtaaaaat taataatgag gagaatctag tatgattaca gtaataacat
     3001 tcaacccatc tatagataga atgtataaag tgaataatat aaacataggt gaggtgcaaa
     3061 gggtagtaag taagaatgca actgctggag gtaaaggatt aaacgtagct aaggtatgta
     3121 aaattttaaa agaaaatcct ttagctatgg gatttctagg tggatttaat ggagaattta
     3181 taaaaaatga acttagaaaa ctagatatac aaaataaatt tacaaaaata gagcaggaaa
     3241 ctagaaattg cttaaacata attgaggaca ataaagttag tacagaattt ttagaaaaag
     3301 gaccaatagt agaaaaaagc gatttagaga gatttgaaaa tgatatagag gaagtactaa
     3361 aaaatactaa aatactagta gcttctggta gttattgtga gaatatgccg atagattatt
     3421 atgaaaagat aggaaatata tgtagagcaa ataatataaa atttatatta gatacatcag
     3481 gagaagcatt aaaaatagct ttaaaatcaa aaccatattt aataaagcca aatatagatg
     3541 aaataagaca tttattagga ataaatatag aatcaagaga ggaaattatc ttgtcaggaa
     3601 aaaaacttat tgaaatgggt gcagagaatg tatgtatatc tttaggaaaa gatggtatgg
     3661 tgtatttaaa cgaatataat gtatatgatg taaaagtacc agttgtaaag tgtataaata
     3721 ctgtaggtag tggagatagc acagtggctg gatttagtgt aggtatttta aggggttata
     3781 aaatagaaaa tttacttaaa tttgcaaatg cttgcggtat aagtaatgct ttaaatatgg
     3841 aaactggatt tgtagatttg ggagaaattg ataaatatca agatttagtt gaagtaaaaa
     3901 agttaagtta aataaattta atgggaatat aataaattta atgttgttgt aatagatttt
     3961 aat
//
LOCUS       DAMJWP010000265         3951 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.272, whole genome shotgun sequence.
ACCESSION   DAMJWP010000265 DAMJWP010000000
VERSION     DAMJWP010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.272"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(132..608)
                     /locus_tag="PC700_003333"
     CDS             complement(132..608)
                     /locus_tag="PC700_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HDF2582601.1"
                     /translation="MSKELVKDRVIKYLIEDLLVPQDMIDTNVELAEFEEGAEGVLDI
                     VVNVKDEEDYYAPVMIVQCLDEDVELEGEVLQKQIEFLEEVDNITMSGRLVLTNGDAM
                     MYADWRGEEYDTEAALPTYDIMVKEFHEMEQQAKDLEEHHHHDKNCGCGCNHHHEN"
     gene            875..2050
                     /locus_tag="PC700_003334"
     CDS             875..2050
                     /locus_tag="PC700_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HDF2582602.1"
                     /translation="MKFKKQISSIVVSTMLVLGSMNLSYADGTNVVTLGANLSESQKQ
                     QMLNYFGVKKDQVLVLDVTNAEERQYLQGVATEGQLGKVTISCSYVEPTKKGNGINVK
                     TANLTWVTSSMIASTLTTAGLEDANVIAAAPYPVSGTGALTGIMKAFEDASGKKLDET
                     KKEIASEELVVTGDLGDDIGQEKATGVMNDIKTEIVKNGTKDTNQIANTINNVVNNYN
                     ITITPEQKEQIKGVMKKIADQNYDYNSMKNTLDNVSDNVNEQLKKLGESVKGSGILDT
                     IGGWFSGIGDWFSGLFSGGDKKDLGILNNTNDESLGSDAVINSTEGVQINPQNNTNSN
                     NDSDTNSSKNDTSSNNENNDSSQTNENNTTNNSENQSNNNDSVGFFEKIKNWLSGLF"
     gene            complement(2131..2934)
                     /gene="proC"
                     /locus_tag="PC700_003335"
     CDS             complement(2131..2934)
                     /gene="proC"
                     /locus_tag="PC700_003335"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDF2582603.1"
                     /translation="MKKIGFIGAGNMASAMIGGIVNSKLVEPNMVIASAYSQGTLDRI
                     DANFGINTTKDSKEVTRTSDIVIVAVKPDIYDDILEEIKDFIDDNKIIVTIAAGKSIK
                     DIESIIGEDKKIVRTMPNTPALVNEAMSSLSINKNINNEDLEAVTEVFNSFGNTEVVP
                     EYLIEAVIGASGSAPAYVFLFIEAIADAAVIAGMPRPQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPGGTTIEAVKVLEEEGFRASVIKAICACIEKSKKMSE"
     gene            complement(2974..3558)
                     /locus_tag="PC700_003336"
     CDS             complement(2974..3558)
                     /locus_tag="PC700_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2582604.1"
                     /translation="MNKDIGAKIKQLRTQKQMTLKDMSEKTNLSIGFLSQLERGLTSV
                     ATDSLGKIASVLDVELTYFFMKPKEHKRAVLRSYEKEVFDVENSTFIHYHLSSSLKEK
                     TMLPRLIEILPSKSSEEICCYVHEGEEFVYVLEGTLTVFLGDEQIEMYPGDTIHYNSE
                     KNNHNWVNYTNKVVKILVVSIPNPFEKSDAVKEA"
BASE COUNT         1333 a          650 c          484 g         1484 t
ORIGIN      
        1 taaccgcaat atcctcctta tatatataat ttattttatt atatgttgtt atattcttag
       61 ttttttgaat gatatatgtc taaaaaaaga ctgtttacta aacagtcttt ttttatatat
      121 atgctcaata tttaattttc atgatgatga ttacatccac atccacaatt tttatcatgg
      181 tgatgatgtt cttctaaatc ttttgcctgt tgttccattt catgaaactc ttttaccatt
      241 atatcataag ttggaagagc tgcctcagta tcgtattctt cccctctcca atcagcgtac
      301 atcatagcat ccccattagt taaaaccaat ctacctgaca tagttatatt atccacttct
      361 tctaaaaatt ctatttgttt ttgtaaaact tctccttcta actctacatc ttcatctaaa
      421 cattgcacta tcattacagg tgcataataa tcttcttcat ctttaacatt tacaacaata
      481 tctaaaactc cttctgcacc ttcttcaaat tctgccaact caacatttgt atcaatcata
      541 tcttgaggga ctaataaatc ttctattaaa tattttatta ctctatcttt tactaattct
      601 tttgacattt atttttctcc ttccgattct ataacattat cataatcatt tctatattat
      661 agtatactac attaattgtt ttttcaatct cttacaaaaa ttattttttt attatcttcc
      721 ttatattccc ttttttatta caaagttgta atcgcattga tttaaaaaat agtttatatt
      781 tagtataata gaaatgtagc acatattttt attcaatata ttaataaaaa aatagattga
      841 aaatgtaatg tatgttaaat ctaggaggaa tattatgaag tttaaaaaac aaattagttc
      901 tattgttgtg tctactatgc ttgtacttgg gagtatgaac ctttcttatg cagatggtac
      961 aaatgtagtt acacttggag ctaacttatc agagtctcaa aagcagcaaa tgcttaatta
     1021 ctttggagta aaaaaagacc aagtcttagt tttagatgta acaaatgcag aagaaagaca
     1081 gtatttacaa ggtgttgcaa cagagggtca acttggaaaa gttactatct cttgctcata
     1141 cgttgaacct actaaaaaag gaaatggaat taatgttaaa acagcaaact taacttgggt
     1201 aactagttca atgattgctt caacccttac aacagctggt cttgaagatg caaatgttat
     1261 tgctgctgct ccatatcctg taagtggtac tggagcattg acaggaatca tgaaagcatt
     1321 tgaggatgct tcaggaaaga aacttgatga aactaagaaa gaaattgctt ctgaagaatt
     1381 ggtagttaca ggtgacttag gagatgatat agggcaagaa aaagcaacag gtgtaatgaa
     1441 tgatataaaa acagaaattg taaagaatgg tacaaaagat accaatcaaa tagcaaatac
     1501 aataaataat gttgtaaata attacaatat tactattaca ccagaacaaa aagaacaaat
     1561 taaaggtgta atgaaaaaaa tagctgacca aaattatgat tacaatagca tgaaaaatac
     1621 actagataat gtatcagata atgtaaatga acaattaaag aaattaggtg aaagtgttaa
     1681 gggttctggt atactagata ctattggtgg atggtttagt ggaataggag actggttctc
     1741 tggtttattc tcaggcggag ataaaaaaga tttgggaatc ttaaataaca ctaatgatga
     1801 gtctcttggc tctgatgctg taataaattc tacagaaggt gttcaaataa atccacaaaa
     1861 taatactaat tctaacaatg atagtgatac aaatagctca aaaaatgata cttcaagtaa
     1921 taatgaaaat aacgatagtt ctcaaacaaa tgaaaacaac acaacaaata attcagaaaa
     1981 tcaatcaaat aataatgact cagttggttt ctttgaaaaa ataaaaaatt ggctttcagg
     2041 cttattttag aaattaaaca aataaaaaga attgcctaaa atggaaattt tctataaaag
     2101 gcaattctct ttatttacaa taaattttta ttactcactc atttttttag atttttctat
     2161 acatgcacat attgctttta tcacagaagc tctaaaacct tcttcttcaa gtactttaac
     2221 tgcttctata gtagttccac caggtgaaca taccatatct tttaattcac caggatgttt
     2281 tcctgtctca agtaccattt ttgcagaacc cataacagcc tgtgatgcaa acttatatgc
     2341 ttgtggtctt ggcattcctg caattactgc tgcatctgct atagcttcta taaataaaaa
     2401 tacatatgct ggtgcagaac cacttgcacc aattacagct tctataagat actctggtac
     2461 tacctcagta tttccaaaag aattaaatac ttctgtaact gcttctaagt cttcattgtt
     2521 tatattttta tttatagaaa gagaagacat ggcttcattt acaagagctg gagtatttgg
     2581 catagttcta actatttttt tatcttctcc tataattgac tctatatcct ttatagattt
     2641 tcctgctgct attgtaacta taattttatt gtcatctata aagtctttta tctcttccaa
     2701 aatatcatca tatatatctg gtttaacagc tacaattact atatcagagg ttctagttac
     2761 ttccttcgag tcttttgtag tatttattcc aaaatttgca tctattctat cgagagttcc
     2821 ttgactatat gctgatgcta taaccatatt aggttcaact aattttgaat ttactattcc
     2881 ccctatcata gcgcttgcca tatttcctgc acctatgaat ccaatttttt tcactttagt
     2941 acctccttat tgactaatag taatcgttaa aatttaagct tctttaactg catcagactt
     3001 ttcaaatgga tttggtatac ttactaccaa aattttcact accttattag tatagttgac
     3061 ccaattatga ttgttctttt cactattata gtgtattgtg tctcctggat acatttctat
     3121 ttgttcatct cccaaaaaca ctgtaagagt accctctaat acatacacaa attcctcacc
     3181 ttcatgtacg taacaacaaa tttcctcact actcttactt ggaagaattt caattaatct
     3241 tggtagcatt gttttttcct ttaaactaga tgataaatga taatgtataa atgttgagtt
     3301 ttctacatca aatacttctt tttcataact tcttaataca gctcttttat gctcttttgg
     3361 cttcataaaa aagtaagtta attctacatc tagtacactt gctatttttc ctaaggaatc
     3421 tgttgccaca cttgttaatc ctctttcaag ttgtgataaa aaaccaattg acaagtttgt
     3481 tttttcactc atatccttca gcgtcatttg cttttgagtt ctgagttgct ttatttttgc
     3541 tcctatgtcc ttattcaaaa ccatcacccc taaataattt taattttttc ataatataaa
     3601 tacaaaaata taaatatagg tactgaacct atctttattt catttaaaaa aaatttttat
     3661 aagtattact ataatttatc aatctttttt tgtctacatt tttacgatta tttacgattt
     3721 atttttcaca tatgtatatt taatcttgcc aactaaagat acatttttta taagcattta
     3781 ctctttatat aataaaactt aagttgttca tgtagtcttc ttctggttta attattaaaa
     3841 tttcaaatta tattattatt tctacacatt attacatcct ttagtacaat tataacataa
     3901 ttttacaatt taatgatatc tattcaatgt ctttttttac tctacaacat a
//
LOCUS       DAMJWP010000266         3947 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.273, whole genome shotgun sequence.
ACCESSION   DAMJWP010000266 DAMJWP010000000
VERSION     DAMJWP010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.273"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            432..1325
                     /locus_tag="PC700_003337"
     CDS             432..1325
                     /locus_tag="PC700_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HDF2582605.1"
                     /translation="MSNKIVSKRQLTDSIYLMEIEAPRVAKSSQPGQFIIIKNDEKGE
                     RIPLTIADYDREKGTVTIVFQTVGASTKKLAMFEENDFVMDFVGPLGQASEFIHEDIE
                     ELRNKKILFVAGGVGSAPVYPQVKWFKEHGLDVDVIIGARTKELIILEDDMKKVAKNV
                     YVSTDDGTYGFNGRVTDLLKDLVDNQGKKYDQAIVIGPMIMMKFMCQLTKELNIPTIV
                     SLNTIMIDGTGMCGGCRVSVGNETKFACVDGPEFDGHLVDFDQAMRRQSMYKTQEGRA
                     ILKLEEGDSHHHSNCGCGGNK"
     gene            1325..2719
                     /gene="gltA"
                     /locus_tag="PC700_003338"
     CDS             1325..2719
                     /gene="gltA"
                     /locus_tag="PC700_003338"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HDF2582606.1"
                     /translation="MDAKKVKVPVREQEPAVRATNFDEVCLGYNKEEAMAEANRCLAC
                     KKPKCVGGCPVGIDIPGFITKIKEDDIEGAAKVIAKSSSLPAVCGRVCPQESQCEGVC
                     ILGIKSDAVSIGKLERFVADWSKENDINLSDTEPKKNQKVAVIGSGPAGLACAGDLAK
                     KGYDVTIFEAMHEPGGVLTYGIPEFRLPKQAVVQPEIDNIRKLGVKIETNVIVGKTIT
                     VDELIEDEGFEAIFIGSGAGLPMFMNIPGENANGVFSANEFLTRVNLMKAYRDDYDTP
                     ISSGKKVAVVGGGNVAMDAARTALRLGSESYIVYRRSEKELPARAEEVHHAKEEGIIF
                     NTLTNPKEILVDENGYVKGMVCIRMELGEPDDSGRRRPIEIEGSEFVLDVDTVIMSLG
                     TSPNPLISSTTKSLNINKKRCLITDENGQTSKEGVFAGGDAVTGAATVISAMGAGKTA
                     AASIDEYLKTKVNA"
     gene            3085..>3947
                     /locus_tag="PC700_003339"
     CDS             3085..>3947
                     /locus_tag="PC700_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HDF2582607.1"
                     /translation="MKKREKVFDGLVFKHILILLILLVLIDLTSTIIYASNSMEITSR
                     NMIEASKRELENYLEVNISLLKALSQDDRFSNGETSLIEKGKLLRPYQKEYNLFMIGI
                     TDTKGNASSTYRENVNSIKDKPSFEKAIKTKQVVVSDIEVSNVTGDKVFIIYVPIVKN
                     NEMIGTIFASFYFQDVNNLISRSNFDDSIKFLMIDKDYTIISHPNKKYVNDKSKMLDL
                     EGNIIGTTKSEILKNINKKCQGDFLSWDNWRLYNVKYTNIKWTNWTLVSKCNIFKNFK
                     NLIVNFMIKLY"
BASE COUNT         1558 a          394 c          756 g         1239 t
ORIGIN      
        1 taatatgaaa gctggtattg caatagatta aagccttata cacgtttagt ttttaaagaa
       61 attagtgtta ttatgaaaac ctaagttaat ttattaaata aataattgaa taaaaaaatt
      121 ttattatgta tttataaaat ttttgttaca aatttggcat aatatagtat actttaagtg
      181 tgtattaggt taaatctata acaaattaga cgtatgatat agacttagtg tttattggca
      241 tttacaagaa atttgttaat atcaactatt tgtagtttgg gcagttataa ttactatagg
      301 ggggatgtcc aatacaaaac aatcaaccaa gaactaatat tatattataa agagttatag
      361 ttttgtgtgt ttatgttttt aattaaattt aaagtgggaa aaatatattt gttagaatgg
      421 agaatagcga gatgagtaat aaaatagtta gtaagagaca attaacagat agtatttatt
      481 taatggaaat agaggcacct agagttgcaa aatcttctca accaggtcaa tttataataa
      541 ttaaaaatga tgaaaaggga gagaggattc ctcttacaat agctgactat gatagagaaa
      601 aaggaacagt aactattgta ttccaaacag ttggtgcttc aactaaaaag ttagcaatgt
      661 ttgaagaaaa cgattttgta atggattttg tagggccatt aggacaagca agtgaattta
      721 tacatgaaga tatagaagaa ttaagaaata aaaagatatt atttgtagct ggtggagttg
      781 gttctgctcc agtttatcca caagttaaat ggtttaaaga gcatggatta gatgtagatg
      841 taataattgg tgcaagaact aaagaattaa ttatattaga agatgatatg aaaaaagtag
      901 ctaaaaatgt atatgtatca acagatgatg gtacatatgg atttaatgga agagttacag
      961 acctattaaa agatttagtt gataatcaag gtaaaaaata tgaccaagca atagtaattg
     1021 gaccaatgat aatgatgaaa tttatgtgcc aattaactaa agaactaaat atacctacta
     1081 tagtaagttt aaatacaata atgatagatg gaacaggtat gtgtggtggt tgtagagtta
     1141 gtgttggaaa tgaaactaag tttgcatgtg tagatggtcc tgaatttgat ggtcatttag
     1201 ttgattttga tcaagcaatg agaagacagt ctatgtacaa gactcaagag ggaagagcta
     1261 ttctaaaact tgaagaaggc gatagtcatc atcatagtaa ttgtggatgt ggaggtaaca
     1321 aataatggat gctaaaaaag taaaagtacc agttagagaa caagaacctg ctgtgagagc
     1381 aacaaatttt gatgaagtat gcttagggta taataaagaa gaagctatgg cagaagctaa
     1441 tagatgttta gcttgtaaaa aaccaaaatg tgtgggtgga tgtccagtag gaatcgacat
     1501 accagggttt ataacaaaaa ttaaagaaga tgatatagaa ggtgctgcta aagtaatagc
     1561 taagagcagt tcattaccag ctgtttgtgg tagagtatgc ccacaagaaa gtcaatgtga
     1621 aggtgtatgt atacttggaa taaaatcaga tgctgtatca ataggtaaat tagaaagatt
     1681 tgtagcagat tggtcaaaag aaaatgacat aaatttatct gatacagagc ctaagaaaaa
     1741 tcaaaaagta gcagtaatag gaagtggtcc agcaggtcta gcttgtgctg gtgatttagc
     1801 taaaaaaggc tatgatgtaa caatctttga agcaatgcat gagccaggtg gagttttaac
     1861 ttacggaata cctgagttta gacttccaaa acaagctgtt gttcaacctg aaatagacaa
     1921 tataagaaaa ttaggtgtaa aaatagaaac taatgtaatt gttggtaaaa caattacagt
     1981 agatgaactt atagaagatg aaggatttga agctatattc ataggttcag gagctggtct
     2041 tccaatgttt atgaatatac caggggaaaa tgcaaatgga gtgttttctg caaatgagtt
     2101 tttaactaga gtaaacttaa tgaaggctta tagagatgac tatgatacac ctattagttc
     2161 tggtaaaaaa gttgctgtag taggtggtgg aaatgttgct atggacgctg ctagaactgc
     2221 attaagatta ggttcagaat catatatagt ttatagaaga agtgaaaaag agcttccagc
     2281 aagagctgaa gaagttcatc atgcaaaaga agaaggcatt atatttaata cattaacaaa
     2341 tcctaaggaa attttagtag atgaaaatgg atatgttaaa ggtatggttt gtataagaat
     2401 ggaattagga gagccagatg attctggaag aagaagacct atagaaatag aaggttctga
     2461 atttgtactt gatgtagaca cagttataat gtcacttgga acaagcccaa atccactaat
     2521 atcttctaca acaaagagtc ttaatataaa taagaagaga tgcttaataa cagatgaaaa
     2581 tggtcagact tctaaagagg gtgtatttgc aggtggagat gctgttactg gagcagcaac
     2641 agtaatatct gcaatgggtg ctggtaaaac agcagcagca tcaatagatg aatatttaaa
     2701 aacaaaagtt aatgcttaat ttgtaaatta aagagagggt gtctcaaaat gaacttttta
     2761 gttcatgagg cacttttttt gtacaaaatc aaatatgttt taatcatttc aacaatgaaa
     2821 atttcattcc tgtagaaagt ctaactttta agtcttagta caatctctat tttgatgtag
     2881 gcactttttt agatggttta ataatttatc agttattatt gtaatatttg atactaagta
     2941 acataacttt gtctaactat gataaaatag ttatacaata aaggtcttgt aaggagtgaa
     3001 taatgtgtgg ctaataaaat cagatttgtc aaatctaaca ttaaataata gaaaaataaa
     3061 ttgtgtaatt aatggtattt taatatgaaa aaaagagaaa aagtatttga tgggctagtt
     3121 tttaagcata tattaatctt attaatacta ttagttttga ttgatttaac aagtaccatc
     3181 atatatgcaa gtaactctat ggaaattact tctagaaata tgattgaggc atcaaaaaga
     3241 gaacttgaaa actatttaga agtaaacata tctttattaa aagctcttag tcaagatgat
     3301 agattttcta atggtgaaac atcactaatt gaaaagggaa aattattaag gccatatcaa
     3361 aaggaatata atctttttat gattggtata acagatacaa aaggaaatgc ttcaagtaca
     3421 tatagggaaa atgttaactc aataaaagat aagcctagtt ttgagaaagc aattaaaaca
     3481 aagcaagtag tagtttcaga tattgaagtc agtaatgtaa caggtgacaa agtatttatc
     3541 atatatgtac caattgtaaa aaataatgag atgattggga ccatttttgc ttctttttat
     3601 tttcaagatg taaataattt aataagtaga agcaattttg atgattctat taaatttcta
     3661 atgatagata aagattatac tattatttct catccaaaca aaaaatatgt aaatgataaa
     3721 agtaagatgc ttgatttaga agggaatatt ataggaacca ctaaaagtga aatcttaaaa
     3781 aatataaata aaaaatgtca aggtgatttt ttatcttggg ataattggag attatataat
     3841 gtaaaatata cgaacataaa gtggactaat tggactttag tatcaaaatg taatatattt
     3901 aagaacttta aaaatctaat agttaatttt atgataaagt tgtattt
//
LOCUS       DAMJWP010000267         3939 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.274, whole genome shotgun sequence.
ACCESSION   DAMJWP010000267 DAMJWP010000000
VERSION     DAMJWP010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.274"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..239
                     /locus_tag="PC700_003340"
     CDS             <1..239
                     /locus_tag="PC700_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005209661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582608.1"
                     /translation="VEESRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKIRRTAIEF
                     STEIMTSLDTLKALVEVKKKHLNKDELKVYNIAE"
     gene            497..1546
                     /gene="carA"
                     /locus_tag="PC700_003341"
     CDS             497..1546
                     /gene="carA"
                     /locus_tag="PC700_003341"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDF2582609.1"
                     /translation="MKARLILEDGTVFIGKAFGYLEESVGEVVFNTSMIGYGEVLTDP
                     SYYGQIVTMTYPLVGNYGINLSSAESEKVQVKGFIVREKSDSPSNFRCEIDIDQYLKQ
                     NKVIGLEGIDTRALTKILRNNGTMKGIITLEDSKLEDVKHKLDKFSNTEAVRTVTRKE
                     VEHIKGNGPKVAVMDFGVKRNILRSFIARGCDITIFPATTSPEDVLSINPDLIFLSNG
                     PGDPEDLEDVIENIKALIGKKPIVGICLGHQLLALALGGKTAKLKFGHRGGNHPVKDL
                     EEGKVFITSQNHGYYVSEVPEQMKVTHINLNDNTVEGMRHEKLDVYSVQYHPEACPGP
                     KDNDYIFDKFLELVK"
     gene            1739..>3939
                     /gene="carB"
                     /locus_tag="PC700_003342"
     CDS             1739..>3939
                     /gene="carB"
                     /locus_tag="PC700_003342"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HDF2582610.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQALKEEGIEV
                     VLVNSNPATIMTDKEIADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     HDAGILDKYNVKVIGTSIESIKKGEDRDLFREVMKEINQPVIVSDIVTNLEAGLEFAN
                     KIGYPVVVRPAYTLGGTGGGIADTEEELREILSHGLQLSPVGQVLLEKSIKGWKEIEY
                     EVMRDGNGNCITVCNMENVDPVGVHTGDSIVVAPSQTLSDEEYQLLRKASIDIINAIE
                     VQGGCNVQIALNPHSLEYAIIEINPRVSRSSALASKATGYPIAKVAAKIALGYTLDEI
                     ENAVTKKTYACFEPTLDYVVVKIPKWPFDKFRHANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHAPSEGRTLEELKARVVVPDDERLFDLAEMIRRGYKVEMISE
                     ITGVDKWFINKFKWIVEQEEKLKVLKIEDLDKEYLHELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIQPVYKMVDTCGGEFEALSPYYYSTYEQYDEVVVSDKRKVVVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMGIETIIVNNNPETVSTDFDTSDKLYFEPLTEEEVLNI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFRDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWTTNEGVEIAKELGYPVLVRPSYVLGGQGMEITYNEEKLIQYL"
BASE COUNT         1630 a          400 c          777 g         1132 t
ORIGIN      
        1 gagtagaaga atctagacca aacatattag atgctataag aaataaacaa gttgatatag
       61 taataaatac accaactaaa ggaaatgact ctacaagaga tggatttaag ataagaagaa
      121 cagctattga attttctact gaaataatga catctttgga tacactaaaa gctttagtag
      181 aagttaagaa gaaacattta aataaagatg agttaaaagt ttacaatata gctgaataga
      241 aattaagttg attgtatagt ttaagaattt aattattaaa taaaaaaaaa gaggttattt
      301 tttctaggag tttttacttt tgagataact tctttttttt aagaaatttg tagtataata
      361 gaaaaagata aaaaataaat gtaaataagt ataaaactag aatgtaaaag tgaaaaatag
      421 atatagatat ttaaaagctt atatttagtt aatgttcttt agtttgtcta aagagaatta
      481 taggaggtac tgtagtatga aagcaaggtt aatattagaa gatggaacag tttttatagg
      541 aaaggcattt ggatatttag aagaaagtgt aggagaagta gtatttaata cttcgatgat
      601 aggatatggt gaagttttaa cagacccttc ttactatggt caaatagtta ctatgactta
      661 tcctcttgta ggaaattatg gtataaattt atcatcagca gagtctgaaa aagttcaagt
      721 aaagggattc atagtaaggg agaaaagtga ttctccaagt aattttagat gtgaaattga
      781 tatagaccaa tatttaaagc aaaataaagt tatcggactt gaaggaatag atacaagagc
      841 tttgactaag atacttagaa ataatggaac aatgaaaggt ataattacat tagaagacag
      901 taaattagaa gatgttaaac ataaattgga taagttttca aatacagaag cagttagaac
      961 tgttacaaga aaagaagtag agcatataaa aggaaatggt ccaaaagtgg ctgttatgga
     1021 ttttggagta aaaagaaata tattaagatc atttatagct cgtggatgtg atataacaat
     1081 atttccagct acaacatctc cagaagatgt attgagtata aaccctgact taatattctt
     1141 gtcaaatgga cctggagacc cagaagacct agaagatgtt atagaaaata taaaagctct
     1201 tattggtaaa aaacctatag ttggaatatg cttaggacat caacttttag ctttagctct
     1261 tggaggaaaa actgctaaac ttaaatttgg gcatagaggt ggaaatcatc cagttaaaga
     1321 tttagaagaa ggaaaagtat ttataacttc tcaaaatcat ggttattatg tttcagaagt
     1381 tcctgaacaa atgaaagtga cacatataaa tttaaatgat aatactgttg aaggaatgag
     1441 acatgagaag ctagatgtat acagtgttca ataccatcct gaagcttgcc caggaccaaa
     1501 agataatgac tatatttttg ataaattttt agaattagta aaataaataa cattagaata
     1561 gctaactaaa aaacaagaat aacttaaatc aaaaagtttg gtttaattaa catagatgta
     1621 ttaaatagat gtattgatga gtaaatttga acttaagata aaataaagta attaaagttg
     1681 attgtataga tgatgaagat aagaactgtg aaaattgttt agataaggag aaagagttat
     1741 gcctaaatta gatagtataa agaaaacttt agtattaggg tcagggccaa taataatagg
     1801 tcaagctgca gagtttgact attcaggaac acaggcctgc caagcattaa aagaagaggg
     1861 aattgaagtt gtattagtaa atagtaaccc agctactata atgactgata aagaaattgc
     1921 agataaaata tatatagaac cattaacaat agaatttata gaaaaaataa tagaaaaaga
     1981 aagaccagac agtttacttg caggtatggg tggtcagaca ggattaaact tagctgtaga
     2041 attacatgat gctggtatat tagataaata taatgtaaaa gttataggaa catctataga
     2101 atctataaaa aagggcgaag atagagattt atttagagaa gtaatgaaag aaataaatca
     2161 accagtaata gttagtgata tagttacaaa tttagaagca ggtcttgagt ttgccaataa
     2221 gataggatat ccagtagttg taagaccagc atatacgcta ggtggaactg gtggaggtat
     2281 agctgatact gaggaagaat taagagaaat actttcacat ggactacagc taagtcctgt
     2341 tggacaagtt cttcttgaaa agagtataaa aggttggaaa gaaatagaat atgaagtaat
     2401 gagagatgga aatggaaact gtataactgt atgtaatatg gaaaatgtag acccagttgg
     2461 agttcataca ggagacagta tagttgttgc tccaagtcaa actctaagtg atgaagagta
     2521 tcaactactt agaaaggcat caatagacat aataaatgca atagaggttc aaggtggatg
     2581 taacgttcaa atagctttaa acccacatag tttagaatat gcgataatag aaataaaccc
     2641 aagagttagt aggtcatcag ccctagcatc aaaagctaca ggatacccaa tagcaaaagt
     2701 agcagctaag atagctttag gttatacttt ggatgagata gaaaatgcag taactaaaaa
     2761 aacttatgct tgttttgagc caacattaga ttatgtggta gtaaaaatac caaagtggcc
     2821 atttgataaa tttagacatg caaatagaaa attaggaact aagatgatgg caactggtga
     2881 aatcatgagt ataggaagta actttgaggc tgctattctt aaagggataa gatcacttga
     2941 aacaggaaaa tattcattgg ttcatgctcc atcagaaggt agaacattag aagaattaaa
     3001 ggctagagta gtagtacctg atgatgagag actatttgac ttagctgaaa tgataagaag
     3061 aggctataaa gtagaaatga tatcagaaat aactggtgtt gataagtggt ttataaataa
     3121 atttaaatgg atagtagaac aagaagaaaa gttaaaagtt ctaaaaatag aagacttaga
     3181 taaagaatat ttacatgaat taaagaaaaa gggattttct gataaaggta tatctgactt
     3241 aatgaagata agcccagaaa aattatatga attgagaagt ttatataata tacaaccagt
     3301 ttataaaatg gttgatacat gtggaggaga atttgaagct ctatcacctt attactattc
     3361 aacttatgaa caatatgatg aggtagtagt tagtgataag agaaaagttg tagtattggg
     3421 ttctggtcca ataagaatag gtcaaggtat agaatttgac tattgttcag ttcactgtgt
     3481 aaaatcttta agaaaaatgg gaatagaaac tataattgta aacaataacc ctgaaactgt
     3541 aagtacggat tttgatacat ctgataagtt gtattttgaa ccattaacag aagaagaagt
     3601 tttaaatata atagaaaaag aaaaaccaga aggtgtaata ctacagtttg gtggacaaac
     3661 agctataaaa ttggctaagt ttttacatga aaaaaatata cctatactgg gaacagactt
     3721 tagagatata gatgcagcag aagatagaga aaaatttgat gatttactag agagactaga
     3781 tataaataga ccaaaaggaa aaggtgtatg gacaactaat gaaggtgttg aaattgctaa
     3841 ggagttaggt tatccagtat tagttagacc atcttatgta cttggtggtc aaggtatgga
     3901 aataacttat aacgaagaaa aactaattca gtatttgga
//
LOCUS       DAMJWP010000268         3891 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.275, whole genome shotgun sequence.
ACCESSION   DAMJWP010000268 DAMJWP010000000
VERSION     DAMJWP010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3891
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.275"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            78..1031
                     /locus_tag="PC700_003343"
     CDS             78..1031
                     /locus_tag="PC700_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDF2582611.1"
                     /translation="MKKRYLVIGIIILSYFSLFIGAEDINIMHIFAKNQHKLMIFIMS
                     RVPRLISILIAGVGMSIAGLIMQQISKNKFVSPTTGATIDAAQFGIVICMLLVPTASI
                     FTKTIIAFVFSLVGTFTFMKIIGKLQFKNIIFVPLVGIMFGNIIGSMTDFIAYKYDLS
                     QNVSSWMQGDFSMILKGNYEILYITIPLIILAYIYANKFTVVGMGMDFATNLGLSYKR
                     IVNIGLIIVALVTVCVVVTAGNIPFIGLIVPNIVSLYMGDNIRASIWYTGLLGAVFVL
                     ICDIFGRIIIYPYEISIGLTVGVIGSILFLYLILRRNVNEA"
     gene            1021..1977
                     /locus_tag="PC700_003344"
     CDS             1021..1977
                     /locus_tag="PC700_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDF2582612.1"
                     /translation="MKLSLNQKIYFVSILLFISIALFIIYGIDMNHLEYALSQRIPKI
                     FAMVLGGGCIAFTTVVFQTITNNQILTPSVLGLDSLYVMIQTIIVFIFGSSSSLIINE
                     NYNFIINVAMMIGASLLLYKMLFEKNKNNIFFLILVGMIFGTLFKSATTFIQVMIDPN
                     EFLALQTSIMASLNNINTNVLLIAFIIIIAIIPFIYDEIKYLDVLSLGKEQAINLGVD
                     FDKAVKKMIILIAILVSISTALIGPMTFLGLLLANITREIFKTYKHTYLISGSMLIGM
                     ITLIIGQFFIQHVFKFDTTLSVVINFIGGIYFIQLLLKGANR"
     gene            1974..2729
                     /locus_tag="PC700_003345"
     CDS             1974..2729
                     /locus_tag="PC700_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2582613.1"
                     /translation="MIEIKNIFKRYKNKNVVDDVSFSIEKGKITSFIGPNGAGKSTVL
                     SIVTRLIGGDGGEVIIEGKSLTNYSNKELAKKIAILKQSNNITLKLTIRELVGFGRFP
                     YSEGNLTKEDENYIDEAIEYMKLTDIQHKYLDELSGGQRQRAYIAMVIAQDTEYILLD
                     EPLNNLDMNHSVQMMKVLRSLCDELDKTIVLVMHDINFASCYSDNIVALKNGKVEKVG
                     RTDEIVNEKVLEDIYEMNFNIKNINGNRICIYF"
     gene            2821..3786
                     /locus_tag="PC700_003346"
     CDS             2821..3786
                     /locus_tag="PC700_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2582614.1"
                     /translation="MNKKAAIVAAVAIIGLVTVFALGGSKKNESKTSENSNNTIKITH
                     NLGETDVKLNPKKVVVFDYSALDTMDALGVAENLVGLPKTSLPASLEKYKDKKYADLG
                     GLKEPDLEGIKSANPDLIIINGRQEDFYEQLSKIAPTISTSKDDKKYLESVKNNIDKI
                     AKIFGVEEKANQEFSKIEKKIETLNKKVTDKNLNALTIMVNEGNLSVFGEESRFSILY
                     NSFGFENKDKNIKESSHGQNITFEYIAKQNPEVMFVIDRGIATGSDVKESSTAKSVLN
                     NDIIKSMDAYKNDNIIYLDSPTWYVNDGGLTSLNKMIDDASKAVN"
BASE COUNT         1560 a          366 c          664 g         1301 t
ORIGIN      
        1 ttattattga taaactataa aagtgaaaat gataataatt ttcattttaa ttaataaaag
       61 atttttaagg agggagaatg aagaaaaggt atttagtcat aggaataata attttgtctt
      121 atttttcttt atttatagga gctgaagata taaatataat gcatatattt gcaaaaaatc
      181 agcataagct tatgatattt atcatgagta gggtaccaag attaataagt atattgattg
      241 ctggtgtagg aatgagcata gcaggtctta taatgcaaca gataagtaag aataaatttg
      301 tatcacctac aacaggtgct acaatagatg cagctcaatt tgggatagta atatgtatgc
      361 ttttagtacc aactgcctct atttttacaa agacaataat agcatttgta ttttctcttg
      421 tagggacttt tacgtttatg aaaataatag ggaagttaca atttaaaaat ataatctttg
      481 taccattagt aggtataatg tttggaaata taataggttc tatgacggat tttatcgcgt
      541 ataaatacga tttgagtcaa aatgttagtt cttggatgca aggtgacttt tctatgatac
      601 ttaaaggaaa ttatgagata ttatatatca caatacccct tataatatta gcatatattt
      661 atgcaaataa atttactgtt gttggtatgg gtatggattt tgcgacaaac ttaggtctta
      721 gttacaaaag aattgtcaat ataggtctga taatagtagc acttgttaca gtatgtgtag
      781 tcgttacagc tggtaatatc ccttttatag gtctaatagt tcccaatatc gtatccttgt
      841 atatgggtga taatataaga gcaagcatat ggtatactgg tttacttgga gcggtatttg
      901 tacttatatg tgatattttt ggaagaatta ttatttatcc atatgaaata tctataggac
      961 ttacagttgg agttatagga agtatactat tcctttattt aatattaagg aggaatgtga
     1021 atgaagctta gtctcaatca aaaaatttac tttgtaagta tattactatt catatcaata
     1081 gcacttttta taatttatgg aatagacatg aatcatttag aatatgcatt atcacaaaga
     1141 ataccaaaaa tatttgctat ggtattaggt ggaggatgta tagcatttac tactgtagtg
     1201 tttcaaacaa ttacaaacaa tcaaatatta acaccaagtg tactaggttt agattcattg
     1261 tatgtaatga ttcaaactat aatagtattc atatttggtt caagcagtag tctgattata
     1321 aatgaaaatt ataattttat tattaatgta gctatgatga ttggagcttc attgttatta
     1381 tataagatgt tatttgaaaa aaataaaaat aatattttct ttttaatcct agtaggtatg
     1441 atatttggaa ccttatttaa aagtgcaaca acatttattc aagtaatgat agacccgaat
     1501 gaatttttag ctttacaaac tagtataatg gcaagtttaa acaatataaa cactaatgta
     1561 ttgttaatag catttataat aattattgcc ataatacctt ttatatatga tgaaataaag
     1621 tatttagatg tattgtcgtt aggtaaagaa caagcaatta atttaggtgt agattttgat
     1681 aaggcagtta aaaaaatgat aatattaata gcaattttag tctcaatatc aacagcactt
     1741 attggaccta tgacattttt aggactttta cttgctaata taacaagaga aatttttaaa
     1801 acatataagc atacatactt gatttcgggt tctatgttga tagggatgat tacattgata
     1861 ataggtcaat tttttataca acatgtattt aagtttgata cgactttaag tgtagtaata
     1921 aattttatag gaggaattta ctttattcaa ttactattaa agggggcaaa tagatgatag
     1981 agataaaaaa tatatttaaa agatataaga ataaaaatgt tgtagatgat gtctctttta
     2041 gtatagaaaa gggaaagata acatctttta taggaccaaa tggagctgga aaaagtacag
     2101 tactatcaat agttacaagg cttataggag gtgatggcgg agaagtaata atagaaggaa
     2161 aaagcttgac taattatagc aataaagaat tagcaaaaaa aatagctatt cttaaacaat
     2221 ctaacaatat aactttaaag cttacaataa gagaattagt tggatttgga agatttccat
     2281 atagtgaagg taatctaact aaagaggatg aaaattatat tgatgaagca atagaatata
     2341 tgaagttgac tgatatacaa cataagtatt tagatgaatt gagtggaggt caaagacaaa
     2401 gagcttatat agcaatggta atagctcaag atacagagta catattactt gatgaaccac
     2461 tgaacaattt ggatatgaac cattccgtac agatgatgaa ggtacttaga agtctttgtg
     2521 atgagttaga taagactata gtattagtaa tgcatgatat aaattttgca tcatgttatt
     2581 cagataatat agttgcatta aaaaatggaa aagttgaaaa agtaggtcgt acagatgaga
     2641 tagtaaatga aaaagtttta gaagatattt atgagatgaa ttttaacatt aagaatataa
     2701 atggaaatcg aatctgtatt tatttttaaa atattgtata tgaaaataag cttgctatat
     2761 gattttttat aaagttttta aattaaaata aagattaata ggagagagga aaatattgat
     2821 atgaataaaa aagcagcaat agtagcagca gtagctatta taggattagt gacagtgttt
     2881 gcattaggtg gaagtaaaaa gaatgaatca aaaacatcag aaaattcaaa taataccata
     2941 aagataactc ataacttggg agaaacagat gtaaaattaa atcctaaaaa agtagtagta
     3001 tttgattatt cggctttaga tacaatggat gcattaggtg tagctgagaa tttagtagga
     3061 cttccaaaaa cgagtttacc agcatcttta gaaaaatata aggataagaa gtatgcagat
     3121 ttaggaggtt taaaagaacc agatttagaa ggtataaagt cggcaaatcc agatttaatt
     3181 ataataaatg gaagacaaga agatttttat gaacaattgt caaaaatagc accaacaata
     3241 agtacaagta aagatgataa gaagtactta gaatctgtta aaaataatat agacaaaata
     3301 gcaaaaatat ttggagtaga agaaaaagca aatcaagaat ttagtaaaat tgaaaagaaa
     3361 atagaaactt tgaataaaaa agtaacagat aaaaatttaa atgctttaac tataatggta
     3421 aatgaaggta atttaagtgt atttggtgaa gaatcaagat ttagtatatt atacaatagt
     3481 tttggatttg aaaataaaga taaaaatata aaagaatcaa gtcatggaca aaatattact
     3541 tttgaatata tagctaaaca aaatccagaa gttatgttcg ttatagatag aggaatagct
     3601 actggtagtg atgtaaaaga aagctcaact gcaaaatctg ttttgaacaa tgatataata
     3661 aaatctatgg atgcatataa gaatgataat ataatatatt tagattcacc aacatggtat
     3721 gtaaatgatg gtggtctaac atctttaaat aaaatgatag atgatgcttc aaaagcggta
     3781 aattaataat ccgatataat caaaatgtat tttttgtaaa atgattatca aataaactag
     3841 gagtttttaa gaactcctag tttatttttt gcataataaa gatatagttt a
//
LOCUS       DAMJWP010000269         3863 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.276, whole genome shotgun sequence.
ACCESSION   DAMJWP010000269 DAMJWP010000000
VERSION     DAMJWP010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.276"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            680..>3863
                     /locus_tag="PC700_003347"
     CDS             680..>3863
                     /locus_tag="PC700_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HDF2582615.1"
                     /translation="MATVSGQIVFDRNRSATIDSGDSGIANIPVVLQDIATGVRLVVL
                     TDASGNYVFTNVPNGNYRIVEAFGTTGGVPTPGNFATAIVGPVPIAEMPPISSVTNPP
                     IGSTNLDGTTPNTLLITVAGSDIINQNILNGPVIYTPIENILDSCVSVSNINLITDAD
                     NGTFGFFPPGTPSNTGPEIEPYPNVTPDFTYVVPDPTKFTPLDGEYTVQNIMTNAMSN
                     VIGAWWRIADHTTGNETGRMMVVNGFNPGAVFFKSTVQVTPNTNYLFSTWILNLFKVT
                     GYPPPQLGVRIFDQNNNILYQATLGAQIPVNTNAPEWKEVGTVINSQNNNQLTVEFFS
                     EGESAIGNDYAIDDVSLREIAVPTFTPIKTSNTETANVGDIITYTVTLDNTCDSPLTN
                     VFFRDLIPNGLEFIPGTVTVDGVSVPGVDPNTGFPLPDIGGGIGVEVTFDVVVESIPN
                     PNPTNNIATIDYSYTPVEGGIPNDFSVDSNPVPVEVISADVEVTKLSEPTIVNPGEEL
                     IYTIKVVNNGPFPSENVVLTDDVPASIINPEYSLDGGVTFQPWTGSLNIGTLEVGETR
                     VIIIRGIVNPSTVGIITNTAVVSSTTADPNLNNNTSTIETEVNLLADILVMKTAEPNS
                     AVPGTLLRYTIQVENLGPANAENVILNDDIPASIINPEYSLDGGASFQPWNGSLNIGT
                     LNSGISLTVLIQGTVSLNSSEYIVNTATVSSTTPDPDLSNNISTIITPVNPQAGISII
                     KVADEDVAVPGEEFVYTIEIFNEGPSNATNVVLTDDIPDVILNPEYSLDGGATFQPWN
                     GSLNIGTVAPGQLIRIIIKGLVSSTATGDITNTAEVSADVPEPVTDSSTVTTPIVPSA
                     DIEVIKTSNMDTAVPGETFSYTITVINLGPSAAQSIVLTDDIPDVILNPEYSLDGGVT
                     FQPWNGNLSIGTLDAGEIRSIIIRGTVSQTAVGTIINTATISSPTPDPNPDNNTSTDE
                     TDISPLADISVIKGNEPVAIPGGRFIYGIEIANAGPSFAENVTLTDNIPASILNPEYS
                     IDNGVTFQPWNGSLNIGTLDAGEIRNIIIRG"
BASE COUNT         1480 a          574 c          654 g         1155 t
ORIGIN      
        1 attatatata aaattcagaa aaacatttca gtttacataa taaaaataaa gtaaactaaa
       61 attattgatt ttagattaaa gtgccagatt aagtatttaa gtagatttta agcttgattt
      121 gattagaaag gtacaaacta tcaccgaaaa aaattaaacc catttaaaaa gcaatataaa
      181 agcttgttat tttcgagaaa aatagaccct ctaaactcaa ttagataaaa ataaaataca
      241 aaaaattata tctctgaata tctcaagtaa ttaagtttaa attttacgat attttgtttg
      301 atgatttata atataaaagc atattcttaa tcttctcaaa ttaaaaagag taaaaaataa
      361 caaaaaaatc actgacattt cactgacaaa ttgtaatttt gttattataa atacattgaa
      421 aatacttgat tattgaaaaa tggcataaaa taataccttt tatgccattt actattgttt
      481 gatagttttt aacttatgac ataagatatt ttttaacata tacaataaaa tataaaaaaa
      541 ttgatattgc aaataaaaaa aatacatatt agatgttata gaaaaaaagc gcacttatag
      601 gcttaaaatg tcacataaaa gagtttcaaa gaataatata ttatgaacac aaattttgta
      661 atcacaaagg agtgataata tggcaacagt atcaggacaa atagtttttg atagaaatag
      721 aagtgctacc attgattctg gggactcagg aatagccaat ataccagttg tattacaaga
      781 tatagcaact ggagttagac ttgtggttct cacagatgca tcaggaaatt atgtttttac
      841 taatgttcca aatggtaact atagaattgt agaagctttt ggaactacag gaggagtacc
      901 aacaccaggg aattttgcaa cagcgatagt aggtccagtt ccaattgcag aaatgccacc
      961 tattagttct gtgacaaatc ctcctattgg ttcaacaaac ttagatggta caacacctaa
     1021 cacactatta ataacagtag caggttcaga tataataaat caaaatatat taaatgggcc
     1081 tgtgatttat acacctatag agaatatatt ggattcttgc gtgtctgtat ctaatataaa
     1141 ccttattaca gatgcagata acggaacatt cggttttttt ccaccaggta caccaagtaa
     1201 tacaggacct gaaatagaac cttacccaaa tgtgacgcca gattttacgt atgttgtacc
     1261 agacccaact aagtttacac ctttagatgg agaatataca gtacaaaata ttatgacaaa
     1321 tgcaatgagt aatgttatag gagcttggtg gcgtattgct gaccatacta caggaaatga
     1381 aacaggaaga atgatggtgg taaatggttt taatcctgga gctgtatttt ttaaatcaac
     1441 agtacaagtt acacctaaca ctaattattt atttagcact tggattttaa acctttttaa
     1501 ggtaacagga tatccaccac cacagctagg tgtaagaata tttgaccaaa ataataatat
     1561 actatatcaa gcaacattag gagctcaaat tccagttaat acaaacgcac cagaatggaa
     1621 agaagtaggt actgtgatta attctcaaaa taataatcaa cttacagtag aattttttag
     1681 tgaaggagaa agcgccattg gaaatgacta tgctatagat gatgtttctt taagagaaat
     1741 agctgttcca acctttactc caataaaaac aagtaacaca gaaactgcta atgtaggtga
     1801 tataatcaca tatacagtta cattagataa tacttgtgat agccctttaa ctaatgtatt
     1861 ttttagggat ttaattccaa atggtttaga gtttatacct ggaactgtaa cggttgatgg
     1921 agtatctgta ccaggagtag accctaatac aggttttcca cttccagata ttggaggagg
     1981 aataggggtt gaagtaactt ttgatgttgt ggttgaaagt attcctaacc caaatcctac
     2041 taataatata gctactatag attactccta tacacctgta gaaggcggta taccaaatga
     2101 tttttcagta gattctaatc cagttcctgt tgaagtaatc tcagcagatg ttgaagttac
     2161 aaaattatct gaacctacta tagtgaatcc aggagaggaa ttaatataca ctataaaagt
     2221 agtaaataat ggaccattcc catctgagaa tgtggtttta acagatgatg taccagcttc
     2281 tattataaat ccagagtatt cattagatgg aggagtaact tttcaaccat ggactggaag
     2341 tttaaatata gggacattag aagtaggaga gacaagagtt attataatta ggggtattgt
     2401 aaatccatca acagtaggaa ttataacaaa tacagctgta gtatcttcaa ctacagcaga
     2461 cccaaacctt aataataata catctacaat agagacagaa gtgaatttat tagcagatat
     2521 tttagtaatg aaaacagcag aacctaattc agctgttcca ggtactttat tgagatatac
     2581 tattcaagta gaaaacttag gaccagcaaa tgctgaaaat gtaatattaa atgatgatat
     2641 tccagcctct attataaatc cagaatattc attggatgga ggagcaagtt ttcaaccatg
     2701 gaatggaagt cttaatatag gaactttaaa ctctggaatt tcattaacag ttttaattca
     2761 aggaacagtt agtttaaatt cttcagaata tatagtaaat acagcaacag tttcttcaac
     2821 tacacctgac ccagatttaa gcaacaatat atctaccatt attacaccag ttaacccaca
     2881 agcaggtatt tctattataa aagtagctga cgaggatgta gctgttccag gagaagaatt
     2941 tgtatataca attgaaattt ttaatgaagg accatctaat gctacgaatg tagttttaac
     3001 agatgatata ccagatgtta ttttaaatcc agaatattca ttggatggag gagcaacttt
     3061 ccaaccatgg aatggaagtc ttaatatagg tacagttgca ccagggcaat taataagaat
     3121 tatcataaag ggattagtaa gttcaactgc tacaggagat attactaata cagcagaagt
     3181 aagtgcagat gtaccagaac cagttacgga ttcatcaaca gtgacaactc ctatagttcc
     3241 atcagcagat atagaagtaa taaaaactag caatatggat acagcagttc caggagaaac
     3301 ttttagttat actataacag ttataaattt aggaccatct gctgcacaaa gtatcgtttt
     3361 gacagatgat ataccagatg ttattttaaa tccagagtat tcattggatg gaggagtaac
     3421 tttccaacca tggaatggaa atcttagtat aggcactttg gatgctggag aaataagaag
     3481 tattataata agaggaacag tgagccagac agcagtaggt acaattataa atacagcaac
     3541 aataagttca cctacacctg acccaaatcc agataataat acttcaacag atgaaacaga
     3601 tataagtcca ttagcagata tttctgtgat aaaaggcaac gaaccagttg ctataccagg
     3661 gggaagattt atttatggaa ttgaaattgc aaatgcagga ccatcttttg cagagaacgt
     3721 aactttaaca gataatatac cagcttctat tttaaatcca gagtactcaa tagataatgg
     3781 agtgactttc caaccatgga atggaagcct taatataggt actttagatg ctggagagat
     3841 aagaaatatc ataataaggg gaa
//
LOCUS       DAMJWP010000270         3841 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.277, whole genome shotgun sequence.
ACCESSION   DAMJWP010000270 DAMJWP010000000
VERSION     DAMJWP010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.277"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            71..1183
                     /locus_tag="PC700_003348"
     CDS             71..1183
                     /locus_tag="PC700_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2582616.1"
                     /translation="MNAFIRKRNKNYVVYLEFEDEKTGKRKQKNMGTFDKKREASKRL
                     NEVKESLYNDGFLVPSEITVSEFLLDFLNKYSENISLATYNNYVSICKNYINPSIGKY
                     KIQDLHPVHIQNYIDKLSYKLNPQSIKIHINILKLAIKRAYRLKLIKENVMDSIEAPR
                     YKKFKNEIYDREQMIKLLDLAKNTEMELPINLAIGLGLRISEVLGLTWDNIDFEENTI
                     TVNKITSRINGSVILKEPKTESSVRKISSPKELMSLLKEYKIKQNKNLLKSSIRNNNN
                     LLFFNKKCEPIAEDVMSKKFKRFLEKNELPHIRFHDLRHSHVTLLINSKVPIKVISER
                     VGHSNISTTLSVYSHVLKEMDKEASDKISESLFNAN"
     gene            complement(1391..1867)
                     /locus_tag="PC700_003349"
     CDS             complement(1391..1867)
                     /locus_tag="PC700_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582617.1"
                     /translation="MVKKRLVAVVLSLAMISVGNFSYAHSGGHNSSGGHHNKNKSELT
                     RYYCGGHSAHQPNKGFCPYASTKENSGVTISSVHNEKNHKNYFAEKGYNKGYEDGCKG
                     LYNLSVTEAKSDNLYEKEILKSSYEKGYLIGYNEYKAKKVDDVMKKMLDIPMLASI"
     gene            complement(2066..3691)
                     /locus_tag="PC700_003350"
     CDS             complement(2066..3691)
                     /locus_tag="PC700_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2582618.1"
                     /translation="MLQVTGVGLRFGDKELFKDVNLKFTKGNCYGIIGANGAGKSTFL
                     KILSGEIEPNTGSISITDKERMSVLKQDHFEYEEETVLNVVIRGHERLWNIMKEKDAL
                     YMKEDFNEEDGIKAAELEGEFAELDGWDAETNAEKILMGLGITKDMHYKQMKELVGGE
                     KVKVLLAQSLFGKPEILLMDEPTNHLDFKSINWLNNFIMDLEESIVIIVSHDRHFLNQ
                     ICTNIVDVDFGKIQMYVGNYDFWYESSQLALQLAKDQNKKTEEKIAQLKEFIARFSSN
                     ASKAKQATSRKKQLEKLEVEDIQPSRRRYPYVGFTPAREIGNEVLEVHNLTKTIDGVK
                     VLDNVSFRLDRDDKVVFMGDEIATTALFNIVMGELEPDSGEYKWGVTTSQDYLPKNHN
                     KFFDGVEYSLVDWLRQFSEEKSESFIRGFLGRMLFSGEEALKEAQVISGGEKVRCMLS
                     KLMLSNANVLVLDDPTNHLDLESITSVNKGLEKFPGVLLFTSHDHEFISTIANRIIEI
                     TPNGIMDRKMDFDEYLESKEIQDQLAKMYGKDK"
BASE COUNT         1342 a          610 c          425 g         1464 t
ORIGIN      
        1 tatttcaaaa ttattttgtg atatatatat gttataataa atgtataaaa ttttgattgg
       61 tgggttatat atgaatgcat ttattagaaa aagaaataaa aactatgttg tttacctgga
      121 atttgaagat gaaaagacag gtaaaagaaa acaaaaaaat atgggtactt ttgataaaaa
      181 aagggaagca agtaaaagac taaatgaggt aaaagaaagc ttatataatg atggtttttt
      241 agtaccaagc gaaattacag tgtcagaatt tctattagat tttctaaata aatatagtga
      301 aaatatatct ttagctacat ataataatta tgttagtatt tgtaaaaatt acattaatcc
      361 atctattgga aaatataaaa tacaagactt acatccggtt cacatacaaa actacataga
      421 taaactatct tataaactaa atcctcaatc aattaagatt catataaata tattgaaact
      481 tgcaataaaa agagcttata gactcaaatt aataaaagaa aatgttatgg atagtataga
      541 ggctccaaga tataaaaaat ttaaaaatga gatttatgat agagaacaaa tgataaaatt
      601 attagattta gcaaaaaata cagaaatgga attacctatt aatttggcta ttggattagg
      661 tcttagaatt tcagaagttt taggtctaac ttgggataat attgattttg aagaaaatac
      721 aataacagta aataagataa cttcccgaat taatggttct gttattctca aagagccaaa
      781 gacagaaagc tctgttagaa aaatatcttc tcccaaagaa ttaatgtctt tattaaaaga
      841 atataaaata aaacagaata aaaatttatt aaaatcttcc attagaaata ataataattt
      901 gttgtttttc aataagaaat gtgaacctat tgctgaagat gttatgagta aaaaatttaa
      961 aagattttta gaaaagaatg aattacctca tattagattc catgatttaa gacactcaca
     1021 tgttacttta cttataaatt ctaaagtacc tataaaggtt atatccgaga gagtaggaca
     1081 ctcaaatata agtactactc taagcgttta ttctcatgta cttaaagaaa tggataaaga
     1141 ggcttctgac aaaatttctg aaagtttatt taatgctaat taatattaat ttatcttagc
     1201 tttaaattag cttagcttat tgagatagat tcattaatat taaatgtatc tatctcaaat
     1261 taataactac ttaaataatc tgttttattt taatttatcg cctcagttat tatacttttt
     1321 tatgaaaaat tatactttac aataaaaaag atatttcctt atagaaagta tctttttaaa
     1381 tactatttta ttatatactt gctaacattg gaatatctaa catcttcttc attacatcat
     1441 ctactttttt tgctttatat tcattgtatc caattaaata tcctttttca tatgaacttt
     1501 ttaaaatctc tttttcatat aaattatcag atttggcttc tgtgacactt aaattataaa
     1561 gccctttaca cccatcttca taacctttat tatacccttt ttctgcaaaa tagtttttat
     1621 gatttttttc gttatgtact gaagatattg ttactccaga attttctttt gtactagcat
     1681 aaggacaaaa acctttattt ggttgatgtg ctgaatgtcc tccacaataa taacgtgtta
     1741 actctgattt atttttatta tgatgacctc ctgatgaatt atgccctcct gagtgagcat
     1801 atgaaaaatt ccctacacta atcatagcta aactcaatac tacagctact aaccttttct
     1861 taaccataat ctattccccc caaaatgtta tatattttta ttatatcatc tactatatta
     1921 tatttaaatt cttaatatac aaaatattac tcgattctgc taataaacat atttaaacaa
     1981 cataaaaaac ctcctaatat ttgattaaaa atttacacac tcatgcatag catgtatcaa
     2041 atattaaaag gttttatact aataattatt tatcttttcc atacatttta gctaattggt
     2101 cttgtatctc tttactttct aaatactcat caaagtccat ttttctatcc attattccat
     2161 ttggagttat ctctataatt ctatttgcta tagtagatat aaattcatgg tcatgagaag
     2221 taaataaaag cactcctggg aacttttcaa gtcctttatt tacagaagtt atactttcta
     2281 ggtctaagtg gttagtaggg tcatctaata caagtacatt tgcatttgaa agcattagct
     2341 tactaagcat acatctaacc ttttctcctc cagatataac ttgagcttct tttaatgctt
     2401 cttcacctga gaataacatt ctacctaaaa atcctcttat aaaactttca cttttttctt
     2461 cagagaactg tctaagccag tcaaccaaag aatattctac tccgtcaaaa aacttattat
     2521 gattttttgg aagataatct tgagaagtag ttactcccca tttatactct ccactgtctg
     2581 gttcaagttc tcccataact atgttgaaaa gtgcagtagt agctatttca tctcccataa
     2641 atactacttt atcatctcta tccaatctaa atgacacatt atcaagaacc ttaaccccat
     2701 ctatagtttt agttaaattg tgtacttcta aaacttcatt cccaatttct cttgcaggtg
     2761 taaatcctac atatggatat cttcttcttg atggttgtat atcttcaact tctaatttct
     2821 ctaactgttt ctttcttgat gttgcttgtt tagcctttga agcattagaa ctaaatctag
     2881 ctataaattc ctttaattga gctatctttt cttcagtctt tttattttgg tccttagcca
     2941 gttgtaaagc caattgactt gactcatacc agaaatcata gtttccaacg tacatttgta
     3001 ttttaccaaa atctacgtca actatattag tacatatttg atttaggaaa tgtctatcat
     3061 gtgatactat tataactata gactcttcta aatccattat aaaattattt aaccagttta
     3121 ttgatttaaa atctaagtgg tttgtaggtt cgtccattaa tagtatttca ggcttaccaa
     3181 aaagtgattg tgctaacaat accttaacct tttccccacc aactagttcc ttcatttgtt
     3241 tgtaatgcat atctttcgtt atgccaagac ccataagtat tttctcagca ttagtttctg
     3301 catcccatcc gtcaagctct gcaaactcac cttcaagttc ggcagctttt atcccatctt
     3361 cttcattaaa gtcttccttc atatacaatg catctttttc tttcattatg ttccaaagtc
     3421 tctcatgacc tcttataaca acatttaaaa cagtttcttc ttcatattca aagtggtcct
     3481 gttttaaaac tgacattctt tctttatcag ttatagatat actacctgta ttaggctcta
     3541 tttctcctga taatattttt aaaaaagtag attttcccgc accattagct cctattattc
     3601 catagcaatt acctttagtg aattttaagt taacatcttt aaatagttcc ttatcaccaa
     3661 atctaagtcc aacacctgta acttgtaaca ttaatatatt ttcctttctt tttgctcata
     3721 atttatttta tcataaaact ttgataacat ttttattata tattatcacg taaatagtat
     3781 attttttata ctaaatttgt gaaatattta ttaaaataat actaaaaatt gtaaatttat
     3841 a
//
LOCUS       DAMJWP010000271         3838 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.278, whole genome shotgun sequence.
ACCESSION   DAMJWP010000271 DAMJWP010000000
VERSION     DAMJWP010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.278"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            95..1585
                     /locus_tag="PC700_003351"
     CDS             95..1585
                     /locus_tag="PC700_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2582619.1"
                     /translation="MFQFETQIHKLRHDVLTAIARLGKEDNLTREAMDNIQYEIIKGE
                     KPTYRCCVYKERAIISERVNVSMGLQPGQDKIDLDTMDIEEDEQIIYILEAACDSCPI
                     NEFTVTDICRGCLAHRCKEACKFGAITHVGGMAYINHELCKACGMCKKACQYDAISEV
                     VRPCKSVCPTNALGFDRENMKAMIHEDKCLNCGACMSACPFGAISDKSLIAPVARKLV
                     QKEKMYAVVAPAITGQVEANVTYGQIKNAIKSLGFVDVLEAACGADAVTVHESLEFAE
                     RMEKGEAFMTNSCCPGFVGYIEKTYPNHVGKISSTVSPMIASGRFIKSHEPDAKVVFI
                     GPCTAKKSEAAKKELKGAVDYVLTFEEIMALFEAFDIDLAKCSNEDIDDASIFGRGFG
                     AAGGLTKAIENYLAEKGIGVNFEPVKISGSREIKKTMTMANLGKLEGNFIEGMMCEGG
                     CINGSGKFLSGAKVKATFDRKNSQSTKKSVLANTKIKDFSNINLEK"
     gene            1843..3339
                     /locus_tag="PC700_003352"
     CDS             1843..3339
                     /locus_tag="PC700_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2582620.1"
                     /translation="MFEFETQLKRLKHEVLTAVARLSIEENLTKENIEKIPYEIIKGE
                     KARYRCCVYKEREVVLERAQLALSLTPNSKYGDINPDSVSLDTDEQIIYIVEAACDRC
                     PINEFTVTELCRGCLAHRCKEACKFGAISYINGRAYINHDLCKACGMCKSSCQYDAIS
                     EVVRPCKSVCPTGALDFNRNTMKAMIHEDNCINCGACISACPFGAISDKSLIAPVAKK
                     LAKKENMYAIVAPAITGQIDSNITYGQLKNAIKSLGFVDMIEASCGADAVTVHESNEL
                     IERIKSGDNYMTNSCCPGFFKYIEKMFPDEKDRISSTVSPMVATGRLVKKIDSDAKVV
                     FIGPCTAKKSEATEKSLKDSVDYVLTFEELMALFEAFNIDLSKCSNEDINDGSIFGRG
                     FGASGGLAKSVQNYIAEKGLSFEFEPIKVSGPRQIKKTMTMAKLGRLEGNFIEGMMCE
                     EGCINGAGKFLSGVKAKNTFDRKNGQSTKKSVLSNDKIKEFEDVNLER"
     gene            3473..3823
                     /locus_tag="PC700_003353"
     CDS             3473..3823
                     /locus_tag="PC700_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2582621.1"
                     /translation="MKKIVLKDILFLVTTLSIIVILLFVTNITTNSKIKSTSESYLKS
                     DKNSVRQNGIQRSTKNTAFKTTILSTGKSDCILIEIGDKFIMIDTGEDKNGEQIVDKL
                     KEKEINALDYLIKK"
BASE COUNT         1507 a          393 c          762 g         1176 t
ORIGIN      
        1 atttaaacat ataatatttt atgtatatat taataaaagg acatattgtg ttataatggt
       61 atcgtataaa atcatttacg ggggtggtat taaaatgttt caatttgaaa cacaaataca
      121 caaattaaga catgatgttt taactgcaat agctagactt ggaaaagaag ataaccttac
      181 cagagaagca atggataata ttcagtatga aataataaaa ggtgagaaac ctacatacag
      241 atgttgtgtt tataaagaaa gagctataat atctgagaga gtaaatgtaa gtatggggtt
      301 acaaccaggt caagataaga tagatttaga tacaatggat atagaagaag atgaacaaat
      361 tatatatatt ttggaagctg catgtgacag ttgtccaata aatgaattta cagtaacaga
      421 tatttgtaga ggatgtctag cacatagatg taaagaagct tgtaaatttg gagctataac
      481 acatgtcgga ggaatggcct atataaatca tgaattatgt aaggcttgtg gaatgtgtaa
      541 aaaagcttgt caatatgatg ccatctcaga ggttgttaga ccatgtaaga gtgtttgtcc
      601 aactaatgcc ttaggatttg atagagaaaa tatgaaagct atgatacatg aggataaatg
      661 tttaaactgt ggagcatgta tgtcagcatg tccatttgga gcaatatctg ataagagttt
      721 gatagctcct gtagctagaa agttggttca aaaagagaaa atgtatgcgg tagtagctcc
      781 agctataaca ggtcaagttg aagcaaatgt tacatatggt caaataaaaa atgctataaa
      841 atcactagga tttgttgatg tattagaagc agcttgtgga gcagatgcag ttacagttca
      901 tgaaagctta gaatttgctg aaagaatgga aaagggagag gcttttatga caaattcttg
      961 ttgtccagga tttgttggtt atatagaaaa aacataccca aatcatgtag gaaaaatatc
     1021 ttctacagtg tctcctatga tagctagtgg tagatttata aaaagtcatg aaccagatgc
     1081 taaagttgta ttcataggac catgtacagc caaaaagagt gaggcagcta agaaagaatt
     1141 aaaaggagct gttgattatg tacttacttt tgaagaaata atggcattat ttgaggcatt
     1201 tgatatagac ttggctaagt gttcaaatga agatatagat gatgcatcaa tttttggaag
     1261 aggttttgga gctgctggtg gtcttacaaa agctatagaa aattatcttg ctgaaaaagg
     1321 tattggtgtt aattttgaac ctgtaaagat ttctggttca agagaaatca agaaaactat
     1381 gactatggct aatttaggaa agctagaagg taattttata gaaggaatga tgtgtgaagg
     1441 tggttgtata aatggttctg gtaaattctt atcaggtgca aaggttaaag ccacttttga
     1501 tagaaaaaat tctcaatcaa ctaagaaaag tgtcttagca aatacaaaga taaaagattt
     1561 tagtaatata aatttagaaa aataaagata atacaaagca acaaaaatac atattgttaa
     1621 tagagttgtt ctaagttgag gtttaacttt tggacaactc tatttttatt tatatattta
     1681 attaatgaac aactaaaata aaaattacat atataatatt ttgtatataa cttaacaaag
     1741 tatatgccta tgttataata atttatagaa ataaataaaa aatacaatat acataatagt
     1801 agaaatatag taaaaaggtg tgatttatag ggaggattac ttatgtttga atttgaaacg
     1861 caactaaaaa ggttgaaaca tgaagtttta acagcagtag caagactatc tattgaagaa
     1921 aatttaacaa aagaaaatat agaaaaaatt ccatatgaaa taattaaagg agaaaaggcg
     1981 agatatagat gttgtgttta taaagagaga gaagtagttc ttgaaagggc gcaacttgca
     2041 ttaagtttaa ctccaaatag taaatatgga gatataaatc cagattcagt tagtttggac
     2101 acagatgagc aaattatata tatagttgaa gcagcatgtg atagatgtcc aataaatgaa
     2161 tttacagtta cagagctttg tagagggtgt ttggctcata gatgtaagga agcatgtaaa
     2221 tttggggcaa tttcttatat aaatgggaga gcatatataa atcatgattt atgtaaggct
     2281 tgtggaatgt gcaaaagttc atgtcaatat gatgctatat cagaagttgt aagaccttgt
     2341 aaaagcgttt gtccaacagg agctttggat tttaatagaa acactatgaa agcaatgata
     2401 catgaagata attgtataaa ttgtggagca tgtatatcag catgtccttt tggagcaata
     2461 tctgataaga gtttgatagc acctgttgca aaaaaacttg caaagaaaga aaatatgtat
     2521 gcaatagtag ctccagctat aacaggtcaa atagatagta atattacata tggtcagtta
     2581 aaaaatgcta taaaatcact tggatttgta gatatgatag aagcttcttg tggagcagat
     2641 gcagttacag ttcatgaaag caatgagctt attgaaagaa taaaatcagg agataattac
     2701 atgacaaatt catgttgtcc gggatttttt aagtatatag agaagatgtt cccagatgaa
     2761 aaagatagaa tatcatctac agtgtctcct atggtagcaa caggaagatt agtaaaaaaa
     2821 atagattctg atgctaaagt tgtatttata gggccatgta cagctaaaaa gagtgaggca
     2881 acagagaaaa gtttaaaaga ttcagtagat tatgtactta cttttgaaga actaatggca
     2941 ttatttgaag catttaatat agatttatca aaatgttcaa atgaagatat aaatgatggt
     3001 tcgatttttg gaaggggatt tggagcgtct ggtggacttg ccaaatcagt acagaactat
     3061 attgctgaaa agggacttag ttttgagttt gaacctataa aagtatctgg tccaagacaa
     3121 attaaaaaga caatgacaat ggctaaattg ggaagattag aaggtaattt tatagaagga
     3181 atgatgtgtg aagaaggttg tattaatgga gcaggtaagt ttttatcagg tgtaaaagct
     3241 aaaaatactt ttgatagaaa aaatggacaa tctactaaaa agagtgtttt atctaatgat
     3301 aagattaaag aatttgaaga tgtaaattta gaaagataat tgttttggta cagtcatttt
     3361 tttatcatgc cacaaatatg cctaaaattt tcccttttta tatgtgtatt ttacagagtt
     3421 aaggttgcta tactataaat atgtaataaa taaaatttat aggagctttt ggatgaagaa
     3481 aatagtatta aaagatatcc tgtttttagt tactacatta tctataatag taatattatt
     3541 gtttgtaaca aatataacta ctaatagtaa gataaaatca acaagtgaat cttatttaaa
     3601 aagtgacaaa aatagtgtga ggcaaaatgg tatccagagg agcacaaaaa atactgcatt
     3661 taagactact atattaagta ctggaaagtc tgactgtata ttaatcgaga taggtgataa
     3721 attcataatg attgatacag gagaagataa aaatggagaa caaatagttg ataaattaaa
     3781 agaaaaagaa ataaatgcac ttgactattt gattaaaaaa tagctacgca cactcgtg
//
LOCUS       DAMJWP010000272         3779 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.279, whole genome shotgun sequence.
ACCESSION   DAMJWP010000272 DAMJWP010000000
VERSION     DAMJWP010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.279"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            60..734
                     /gene="fsa"
                     /locus_tag="PC700_003354"
     CDS             60..734
                     /gene="fsa"
                     /locus_tag="PC700_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDF2582622.1"
                     /translation="MQLILDTGNVEEIKELCTCLPIDGVTTNPSIVSKEKKNFKQLIN
                     EIGEIIGEDMPIHAQVLSTKYEEILDEALYISSLRKNIYVKIPVTQDGLRAIKDLHRK
                     GVKITATAIFTAHQGFLAAKAGANYIAPYVNRLDNISGDGVAMVSELIKIIDTYKMDT
                     KVLAASFKNAQQVIELMQHGVHSVTVPYDICKSMMNHPLTDWSVDKFIEDWENTFGKG
                     SKTNNI"
     gene            complement(856..2250)
                     /locus_tag="PC700_003355"
     CDS             complement(856..2250)
                     /locus_tag="PC700_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HDF2582623.1"
                     /translation="MGIILKPKQFYMTVIVTIILYFSLNYSFNIKFSICLSFLTILIW
                     AVDSVEKTFVALFFVILAFIFNIAPIKVILQFLFTENFYIIILAYIITNAVTKTGVAK
                     IISEKLILNTVNTPKKMILLSYILGILLIFFIPQPFPRVILVSAFYKEFLKEQQITED
                     SKSILLFSIFTASTFTSMFFVNGDTLLNYVVLELGNCNINWGQWAFYMSVPTIITCFI
                     TYFLFIFVFKKELHSIKFISNENLEGNKLHFSYSNLKKEFNLLNKEQKHVFLCLGIMF
                     FMFLTQFIHNINTLIIMSICVLLLLLRKIVGFSTLKEINWKVLIFFIAAFSIGGVLKY
                     SGVVDIMGNYLIKFIPNSSEIISILFLITLTIILNICLGSAVTTSSVVIPLLGSLHIL
                     KENSVTLCLFVYIIVSIQYILPFHHATIMVGHGENLYNSKVIFKYGLTLIPLTYFIII
                     CITFPWWKFIGLEI"
     gene            complement(2328..3674)
                     /locus_tag="PC700_003356"
     CDS             complement(2328..3674)
                     /locus_tag="PC700_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2582624.1"
                     /translation="MKDLTTGHEGKSIFFFAMPMLIGSLFQQLYNTADSIIVGRFIGK
                     EAMAAVSGANPIMFLLVAALMGVSLGFSILVSQFYGSGDLKKVKATIDTTYILLFIGS
                     ILISILGIVFGGPMLKLMNTPESVFAQSKLYLTIIFSGILFSAGYNSVSAILRGLGDS
                     VTPLYFLIIATILNIVLDLTFIVVLRMGVEGVALATIMAQAVSFIISIIYLNKKHEVL
                     KFKIKGIVYDNKIFKDGLRLGLPSGIQQMLFSIGNMTLQFLVNSYGTSAMAAFGAGLR
                     IENFISLPIMNLGSAVSTFVAQNIGAGENERVKKGIRESIKMTLVLAVTVIALILLFR
                     ENLIALFNTDKDVIKIGSSYLFIIGPFFLFIGTSFVLSSAMKGAGDSMFALISSIVSL
                     WLGRLPASYMLSKFFGTDGIWMGIPFGWTLGLIVTVIYYKKGYWKTKAIVNHRINE"
BASE COUNT         1487 a          531 c          523 g         1238 t
ORIGIN      
        1 aaaataatac caaatcaata tataataaga ataaagaaag attaaagggg gatattttaa
       61 tgcaactaat cttagataca ggtaatgtgg aggagattaa ggagttatgt acttgtttac
      121 caatagatgg tgtaactaca aatccaagta ttgtttcaaa agaaaaaaag aattttaagc
      181 aacttataaa tgaaattggt gagattatag gtgaggatat gcctatacat gctcaagtgt
      241 taagtacaaa gtatgaagaa atattagacg aagcattata tataagtagt ttgagaaaaa
      301 atatatatgt aaaaatacct gtaacacaag atggtttaag agctataaaa gaccttcaca
      361 gaaaaggtgt aaaaataact gctactgcaa tattcactgc tcatcaaggt tttttagctg
      421 caaaggctgg agcaaattat attgcacctt atgtaaatag gcttgataat atatctggtg
      481 atggagtagc tatggtttca gaacttataa aaataataga tacttataaa atggatacaa
      541 aagtacttgc agccagtttt aaaaatgcac aacaagttat agaattaatg caacatgggg
      601 tgcatagtgt tacagtacca tatgatattt gtaaatctat gatgaatcat ccattaactg
      661 attggagtgt agataagttt atagaggatt gggagaatac ttttggaaaa ggaagtaaaa
      721 caaataatat ttagtattat ggtaaaagag tagttttgtc caatttcaca agaacttata
      781 taaaaagatg aaaatatgaa ttttcttttt aataatttcg ttattatctt aagttaattc
      841 atattttcat cttttttaga tttctagtcc tataaatttc caccaaggga atgttataca
      901 tattattata aagtaggtta aaggaatcaa agttaaaccg tatttgaaaa taaccttgct
      961 attatacaga ttttcaccat gaccaaccat tattgtagca tgatgaaatg gtaatatgta
     1021 ctgtatgctt actataatgt agacaaatag acataaagta acactatttt cctttagtat
     1081 atgtaaactt ccaagtaatg gtattactac agatgatgta gtgacagcac ttccaagaca
     1141 tatattcaag attattgtga gagttatcaa aaacaatatt gaaataattt ctgaagagtt
     1201 tggtataaac tttatcaaat aatttcccat tatatctaca actccagagt actttaacac
     1261 tcctccaatt gaaaatgctg ctataaaaaa aataaggacc ttccaattta tttcttttaa
     1321 agtactaaat ccaactattt ttcttagtaa taaaagaagt acacaaattg acattataat
     1381 taatgtgttt atgttatgta tgaactgagt taaaaacata aaaaacatta ttcctaggca
     1441 taaaaatacg tgtttttgct ctttgtttag caaattaaat tcttttttta aattagaata
     1501 tgaaaaatga agtttattac cctcaagatt ttcattagaa ataaatttta ttgaatgcaa
     1561 ttctttttta aagacaaaga taaataaaaa ataagttata aaacaagtaa ttatagttgg
     1621 aacagacata taaaaagccc attgacccca atttatatta caatttccaa gttctagtac
     1681 aacataattt agaagggtgt ctccatttac aaaaaacatt gatgtaaagg tagatgctgt
     1741 aaatatacta aataaaagta tagattttga atcttcagtt atctgttgtt cttttaaaaa
     1801 ctccttgtaa aaagcagata caagaataac ccttggaaat ggttgtggga taaaaaatat
     1861 taaaagaata cctaaaatat acgataaaag aatcatcttt ttaggggtat ttacagtatt
     1921 taaaattaac ttctcagaaa taatttttgc gacacctgtt tttgtaactg cattagtaat
     1981 tatgtatgca agaattatta tgtaaaaatt ttctgtaaat aaaaactgta aaataacctt
     2041 tattggtgca atgttaaata taaacgctag aattacaaaa aacaatgcaa caaaagtttt
     2101 ttcaactgag tctacagccc atatcaagat agttaaaaaa gataagcata tagaaaattt
     2161 tatgttgaaa ctgtaattaa gagagaagta gagaattatg gtaactataa cagtcatata
     2221 aaattgtttt ggcttaagaa taatacccat ttttatattc acctccataa tttattatat
     2281 cataatggag gtgaatacaa tgaatgtgaa tacattttat aaaaagatta ttcattgatt
     2341 ctatgattta caatagcttt agttttccaa taccctttct tatagtagat aactgtaaca
     2401 ataagaccta aagtccaacc aaaaggaata cccatccaaa ttccgtctgt tccaaaaaat
     2461 ttagaaagca tataagaagc tggaagtctt ccaagccata aagatactat tgagcttata
     2521 agtgcaaaca tggaatcacc agcacctttc attgcacttg aaagtacaaa agaagttcca
     2581 ataaataaaa agaatggtcc tattataaat aagtaagaag agccaatttt aatgacatct
     2641 ttatcagtat tgaatagagc aattagattt tccctaaata gcaaaattaa agcaataact
     2701 gtaactgcta aaactaaggt catttttata gattctcgaa tgcctttttt aaccctttca
     2761 ttttctccag cacctatgtt ttgagcaaca aaagtagaaa cagcagaacc aagattcata
     2821 ataggtaagc ttataaaatt ttctatccta agaccagcac caaaagctgc catagcagat
     2881 gtaccataac tattaactaa aaattgaaga gtcatatttc caattgaaaa caacatttgt
     2941 tgaattccac ttggaagtcc taatctaagt ccatctttaa atattttgtt atcgtataca
     3001 attcctttga ttttaaattt aagcacttca tgttttttat ttaaataaat tatacttatt
     3061 ataaatgata ctgcctgagc cattatagtg gcaagtgcaa cgccctcaac acccattctt
     3121 agcactacaa taaaagttaa gtctaaaact atatttagaa ttgtggctat tatcagaaaa
     3181 taaagtggag ttactgaatc tccaagtcct ctaagtattg cacttactga attatatcca
     3241 gcagaaaata atattccact aaatattata gtaagataca attttgattg agcaaaaacg
     3301 gattctggtg tattcataag cttaagcatt gggccaccaa atactattcc aagaatactt
     3361 attaatatag aaccaataaa taaaagtata tatgtagtat caatagtagc ttttaccttt
     3421 tttaaatctc ctgagccata aaattgtgac actaaaatag aaaatccaag gctaacaccc
     3481 ataagcgctg ccaccaataa aaacattata gggttagctc cagagactgc cgccattgct
     3541 tcttttccga taaatctacc aactattata ctgtcagctg tattgtatag ttgttgaaat
     3601 aaactaccaa taagcatagg catggcaaaa aagaatatgg attttccctc atgacctgta
     3661 gttaaatctt tcaccttatt cctccaaata aaaaaattat taatttatta tagtataatc
     3721 ttaacataaa ctttatatcc aaactataaa taaattgtat caaaatatca tatattttt
//
LOCUS       DAMJWP010000273         3770 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.280, whole genome shotgun sequence.
ACCESSION   DAMJWP010000273 DAMJWP010000000
VERSION     DAMJWP010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.280"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(73..609)
                     /locus_tag="PC700_003357"
     CDS             complement(73..609)
                     /locus_tag="PC700_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HDF2582625.1"
                     /translation="MEYKSPVYGIIAVPIEKIESNDYNPNAVAPPEMQLLYKSIKEDG
                     YTMPIVCYYNEEKDKYIIVDGFHRYRVMLENKDIYDRENGLLPVSVIDKSLDERMAST
                     IRHNRARGSHNVDLMSKIITELCEIGKSDEWISKHLGMEVDEILRLKQITGLAYLFKD
                     KEFGNAWIPFGSDIEEEN"
     gene            complement(627..1940)
                     /locus_tag="PC700_003358"
     CDS             complement(627..1940)
                     /locus_tag="PC700_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HDF2582626.1"
                     /translation="MDKRYLESDVFEAFQERLSYLFNEFDYIYVSFSGGKDSGLLLNL
                     VLDYKRKNNISSKIGVFHQDFEAQYSLTTEYVEKVFEENIEDIEPYWVCLPMATRTAV
                     GNYEMYWYPWDDEKEELWVREMPVKEYIINQKNNPFTYYKYKMRQESLAKQFGRWIRD
                     IHEGAKVVCLLGTRAAESLQRYCSIVNKQHGYKGKCWITKEFKNVWTASPIYDWEIED
                     IWLAHYKFDYDYNKIYDLFYKAGVAPRKMRVASPFNDSAKESLNLYRILDPDVWVKLV
                     ARVQGVNFTSIYGKTKAMGYRSVKLPYGHTWKSFTYFLLDTLPENIRNSYIKKFSTSI
                     EFWQNTGGGLSEIVIRELLRKGYKIELNGVSNHTKDKKSRVIFLDDIPDDTDDIKFTK
                     DIPSWKRMCYCILKNDHMCRFMGFGPTKEQRIRTEYIKKKYEKIH"
     gene            2276..2716
                     /locus_tag="PC700_003359"
     CDS             2276..2716
                     /locus_tag="PC700_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HDF2582627.1"
                     /translation="MYERMLDKSNKPCLSQIIEYIGKDGYARLCKLETYLETQYNLSR
                     ELRFPFGNNYGWGYKYSHKSFHLFYAFFEKGAFTVLIQIGDKQVPAVEKTLVSLTPKA
                     KELWVTRYTCGNNGGWIKYRILEDADLLDIIQYIRAKKSPPKQN"
     gene            complement(2901..3089)
                     /locus_tag="PC700_003360"
     CDS             complement(2901..3089)
                     /locus_tag="PC700_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582628.1"
                     /translation="MNKNNNLVIICMFIGMILGMAIGCAIGISKGNVGITMCYGLIFG
                     MIIGICIGTVIKNSNKKE"
BASE COUNT         1302 a          647 c          367 g         1454 t
ORIGIN      
        1 aaagctcttt attttcaata tatattttaa attttctctt tttaatatat ctttttcttt
       61 taataaatct ctttaatttt cttcttctat atcagagcca aagggaatcc atgcattacc
      121 aaactcttta tctttaaata aataagctaa tccagttatt tgcttcaatc taagaatttc
      181 atcaacttcc atacccaggt gctttgatat ccattcatca gattttccaa tttcacaaag
      241 ttcagttata attttactca ttaaatccac attatgagaa cctcttgcac gattgtgtcg
      301 aatagtactt gccattcttt catcaagaga tttatcaatt actgaaacag gaagaagtcc
      361 gttttctcta tcatatatat ccttattttc aagcattact ctatatctat gaaaaccatc
      421 tacaataata tacttatcct tttcctcatt ataataacag acaattggca ttgtataccc
      481 atcttctttt atacttttat acaacaactg catttctggt ggggctactg catttggatt
      541 atagtcgtta gattctatct tttcgattgg cactgcgatg attccatata caggactttt
      601 atattccata gcatcttaac tcctatctaa tgaatttttt catatttctt cttaatatat
      661 tccgttctaa ttctctgttc ttttgtagga ccaaatccca taaatctaca catatggtca
      721 ttttttaaaa tacaataaca cattcttttc caacttggta tatctttggt aaatttaata
      781 tcatccgtat catcaggtat atcatccaaa aatatgactc tactcttttt atcttttgta
      841 tgatttgaaa ctccatttaa ttctatttta taaccttttc tcaacaattc acgtataact
      901 atttctgaca aaccaccacc tgtattctgc caaaactcaa tagatgttga aaatttttta
      961 atatagctat tccttatatt ttcaggtaag gtatcaagca aaaaatatgt gaaacttttc
     1021 catgtgtgtc catatggcaa tttaacacta cgatatccca tagcttttgt ttttccataa
     1081 atagaagtaa aattaactcc ttgtactcta gcaaccaatt tcacccatac atctgggtcc
     1141 aatattctgt ataaatttaa actctctttt gctgaatcat taaaaggtga tgccactctc
     1201 atttttcttg gagctactcc agctttatag aataagtcat aaattttatt atagtcataa
     1261 tcaaatttat agtgggctaa ccatatatct tctatttccc aatcatatat aggagatgcc
     1321 gtccagacat ttttaaattc cttagttatc cagcactttc ctttatatcc atgctgttta
     1381 ttcacaatag aacaataacg ttgtagagat tctgctgctc gtgttcctaa taagcaaaca
     1441 acttttgcac cttcatgtat atctctaatc catctaccaa attgctttgc caaactttct
     1501 tgtctcattt tatacttata ataagtaaaa ggattatttt tttgatttat tatgtattct
     1561 ttgactggca tttctcttac ccataattct tctttctcat catcccatgg ataccaatac
     1621 atttcataat ttcctacagc agttcttgta gccattggta aacacaccca atatggttca
     1681 atatcttcaa tattctcttc aaatactttt tcaacatatt cagtagtcaa actatattga
     1741 gcctcaaaat cttgatgaaa tactcctatt ttggaactta tattattctt tctcttataa
     1801 tccaaaacta aatttagcag taagccacta tcttttcctc cactaaaaga tacatagata
     1861 taatcaaatt cattaaaaag ataagaaagt ctttcttgaa aagcttcaaa tacatcggat
     1921 tctaaatatc tcttatccat ttgaattatc ccctttcaat tagaagttta taaaacctta
     1981 caatacaata ttattatgct tttttagcag taatcagcat aaattataca ttatttaaat
     2041 acacaaatcc aactaaacac atataattat aatgtaatta tacttttgta ttataaacta
     2101 tattttatca ttattttaca tttttttcca atatatataa atatcatatt tttctattag
     2161 ctaaaaataa ttactttata tttttacatg ttagtataat aagacataaa aacttgacat
     2221 actgttgtca taattacttt gctataatta aaagtaaaaa tggaggtaaa ttataatgta
     2281 tgaacgtatg cttgataaaa gcaataaacc ctgtttgtct caaataatag aatatatagg
     2341 taaagatgga tatgcacgat tgtgtaagtt ggaaacttac ttggaaactc aatataattt
     2401 atcaagagaa cttcgatttc cttttggaaa caattatggt tggggttaca aatatagcca
     2461 taaatctttt catttatttt atgctttttt tgaaaaaggt gcatttactg ttctgattca
     2521 gataggtgac aagcaagttc cagcagtaga aaaaacgttg gtatctttga caccaaaagc
     2581 taaagaactc tgggtcactc gttatacttg tggcaataat ggaggttgga ttaagtatcg
     2641 tattttagaa gatgcagact tacttgatat tattcaatat ataagagcaa aaaaatctcc
     2701 acctaaacaa aattaacttt tattttttgt gatttaaata tagtgtatat aaattccatt
     2761 ctttccatat taaaaaaata atataaaaaa tatctattag tatatcttat ttagtatgtc
     2821 ttattctact ttttacagat aattaaaaag ttttatcata aataaatcat aactaaacat
     2881 cctagtaaat caaaattcat ttattctttt ttattagaat ttttgataac agtccctata
     2941 catatgccaa ttatcattcc aaaaattaga ccataacaca ttgtgatacc cacatttcct
     3001 ttagaaatac ctatagcaca acctatagcc attccaagta tcatgcctat aaacatacat
     3061 ataataacta aattgttgtt tttattcata gtttttctcc attcaatata ttttaaattg
     3121 cctagctcgc cttcttagct taaaacaaag aaatataaat taactttctt tcattctaat
     3181 atctgcttgt ataaatgtca aacaacacat ttcttgtcat aacctcctct ttttgaagta
     3241 agtacaatct cccagacaca ataatatcaa tttcctaaaa ccatttaagt aacttttata
     3301 tttacataat atttacaatc ataaactata ttgttattca tttatacatc ataaacactc
     3361 cctcctaatc attattatac ttaaaataat gttcatttat aataatatat ctaacgcact
     3421 ttaattgaaa aaactaaaac tttgcctttc attcccatat ataaataagt atactttttg
     3481 atgctctatt acaaatcaac taaattaatt gatataaatt atcattttct gttgaaaata
     3541 ccatttacac atgctatact taattgcata aatggtaata aatgtcatat gattttacaa
     3601 atctattaat aacaattatt acttttactg tattttaaag tttttggact aaatatcaag
     3661 aactttctaa aatacaaagt acaaaaaatt tatataagtt ataaatttat atgaactaga
     3721 taagctcaaa caatatgtta tgtataaact gtgtagtatt cggttataaa
//
LOCUS       DAMJWP010000274         3764 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.281, whole genome shotgun sequence.
ACCESSION   DAMJWP010000274 DAMJWP010000000
VERSION     DAMJWP010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.281"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..1167
                     /locus_tag="PC700_003361"
     CDS             <1..1167
                     /locus_tag="PC700_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2582629.1"
                     /translation="ELDKNHKDKGNIDKINQDLLNAFWKKGLSLNKFWIEENSLNNID
                     KKSINKIYNQGKTKYSLLVKFIKKDNYLFAISIPIEHSEETIGIVNRFNIIMGIFSVV
                     IITILTFILSNRVIKPMERLKFLSKDISELNFRTEDIKTNDEIEELAYSINIMSEKLE
                     KAHNELNKRNENLKTFISDASHEMKTPIALIKAYAIGMKDGLDDGTYTDTIIEQAENM
                     TNTINSLLYWAKYEKKERSLCLVDLKERLYKSLKNYELLIERGSISVKCSVDDKELII
                     SSDEDSIDMVFNNLISNAIKYTNNNEIEINLFEENERIILSIKNGIDYNMGDDIENMW
                     KPFYVLEKSRSKELSGTGLGLTIVRTILEENNFRYRVEICDGIIEFYIIFIKVI"
     gene            1592..>3764
                     /locus_tag="PC700_003362"
     CDS             1592..>3764
                     /locus_tag="PC700_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF2582630.1"
                     /translation="MKIPKKIAAMLTVTMIAGSSTAGIASAQTVATNLTGQERYETAV
                     KISQDGWKNADEVVIVNDSSIADALSATPFAKAKNAPILLTSKDKLNDKTKAEIQRLK
                     AKKVYLIGGTSVLSTNIEKEIKDLKISFERISGAERYQTSLELAKKLDAISDVKKIAV
                     VNGEKGLADAVSVGAPAAQNNMPVILADSKNGTAVADKFIKDAGITQSYVVGGESSIS
                     EAVKNKLPNSTRLGGTDRNDTNAKVIKEFYKKTDLKNAYVTKDGMNKQDQLIDALAVG
                     VLGAKNQSPVVLVGKNLSASQKSLVNSKSFDKITKVGGNGNETAFNEMKSLQEVKTVE
                     AKTISELKSAIDKATANDVINFKPTSEVKEAFTIQTDKAITVNLNGTYTKTVTINMPN
                     GDVNNYAKVDDVVIDDVKDGTFVNYGKITNLKVNDKNGAKIENNSKGEIGSLTVASGA
                     SQVKVTNGGKITTVTNNSKGTTIDNKGTISSVKGDNSPTISGNSPSSNSSGGSSSSGG
                     SSHGGGSSSSKVDKVVLKNTITAANKLYNEAIEGTNVGEYKVGSKAIYKTAIDKAQAI
                     LDKSGVTQKEVNDAVTALNTATDTFKAGKVVAVDKTALQDAVTAATALHAKATEGTAE
                     GNYAVGSKATYKTAIDEAQAILDKSDATQKEVNDALSALNTATETFEAGKVVAVDKTA
                     LQDAVTAATALHNGATEGTAEGNYAVGSKATYKTAIDEAQAI"
BASE COUNT         1649 a          418 c          673 g         1024 t
ORIGIN      
        1 gaattagata aaaatcataa agataaaggt aatatagata aaataaatca agatttatta
       61 aatgcatttt ggaaaaaagg tttaagttta aataagtttt ggattgaaga aaatagttta
      121 aataatattg ataagaaaag tataaataag atatacaatc aaggtaaaac aaaatatagt
      181 ttgcttgtaa aatttatcaa gaaggataat tatttgtttg ctatatctat acctatagaa
      241 cattctgaag aaactattgg cattgtaaat agatttaata taataatggg aattttttca
      301 gtagttataa taactatact tacatttata ttatcaaaca gagttataaa acctatggaa
      361 aggttaaagt ttttatctaa agatatatct gaattaaatt ttaggactga agatataaaa
      421 acgaacgatg aaatagaaga gctagcttac agcataaaca taatgagcga aaaacttgaa
      481 aaagcacata atgaattgaa taaaagaaat gagaatctaa aaacttttat atcagatgca
      541 tctcatgaaa tgaaaactcc aatagcactt ataaaagcat atgcaatagg tatgaaagat
      601 ggtttggatg atgggactta tacagataca ataattgaac aagcagaaaa tatgaccaat
      661 acaataaatt ctcttttata ttgggcaaaa tatgagaaga aggagagaag tctgtgtctg
      721 gtagatttaa aggaaaggtt atataaaagt ttaaaaaact atgagctgtt gatagaaaga
      781 ggtagtataa gtgttaaatg cagtgtagat gataaagaac ttataataag ttcggatgaa
      841 gatagtatag atatggtatt taataatttg attagtaatg ctataaagta tactaataat
      901 aatgaaatag agattaatct ttttgaagag aatgagagaa ttatattatc cataaaaaat
      961 ggtatagatt acaatatggg agatgatata gaaaatatgt ggaagccttt ttatgtttta
     1021 gagaagtcga gaagtaagga gctttctggt actggtcttg gtcttaccat agttagaact
     1081 atacttgaag aaaataattt tagatacagg gttgaaattt gcgatggaat tatagaattt
     1141 tatattatct ttataaaagt aatttgaatt aaaatgtaaa tataaagaat aattaaaaaa
     1201 atatcgcaat tatttgtttt tcatatggat aaaattggtt atttaatata taattacaag
     1261 tataaataat aaaatttatt tgtaaaaaga gtagcacttt taattctaaa ggctactttt
     1321 tttatacaaa accatgtttt ttataacaat tcattaacat aaaaatcaaa cttaccttaa
     1381 aatataaatt tacaaatttt tcaatcaata agaaaatttc tctattcctt acataatgac
     1441 tgtaatgtaa caaaataatt acaaattaat tatgaaatca ttatagttag aaacctcttt
     1501 aaaattagat attaagtatc ctttagaatt agaacgggaa caaagttaaa aaattaagta
     1561 atatcaaatg gacaaggggg aagaaaataa aatgaaaata ccaaagaaga ttgctgcaat
     1621 gcttacagtt acaatgatag ctggaagttc aacagcaggc atagctagtg cacaaactgt
     1681 ggcaacaaat ttaacagggc aagaaagata tgaaactgct gttaaaataa gtcaagatgg
     1741 atggaaaaat gctgatgagg tagtaatcgt aaacgactca tctatagcag atgctctatc
     1801 tgcaacacca tttgcaaaag caaaaaatgc tccaatactt ttaacaagta aagataaatt
     1861 aaatgataag acgaaagcag aaatacaaag attaaaagct aaaaaagtat acttaatagg
     1921 tggaacttct gtattaagta caaatataga aaaagaaata aaagatttaa aaatatcttt
     1981 tgagagaatc tctggagctg agagatatca aacatcctta gaattagcaa agaaattaga
     2041 tgctataagt gatgttaaga aaatagctgt agttaatggt gaaaaaggac ttgcagatgc
     2101 tgtaagtgta ggagcaccag ctgcacaaaa caatatgcca gtaatacttg ctgattctaa
     2161 aaatggaaca gcagttgcag acaaatttat aaaagatgct ggaataactc aatcttatgt
     2221 agtaggtgga gagagttcaa tttctgaagc tgttaaaaat aaactaccaa atagtacaag
     2281 actaggtgga acagatagaa atgatacaaa tgcaaaagta ataaaagaat tttataaaaa
     2341 gacagattta aaaaatgctt atgtaactaa agatggtatg aataaacaag accaacttat
     2401 agatgcacta gcagtaggtg tattaggtgc taaaaatcaa tcaccagtag ttttagttgg
     2461 aaagaactta tcagcttcac aaaaatctct agttaactct aagagttttg ataagataac
     2521 taaagttggt gggaatggta atgagacagc atttaatgaa atgaagtcat tacaagaagt
     2581 aaaaacagtt gaagctaaaa caatttctga attaaaatca gctatagata aagctactgc
     2641 taatgatgtt ataaacttta agccaactag tgaggttaaa gaagcattta caatacaaac
     2701 tgataaggcg ataacagtaa acttaaatgg tacatataca aagactgtga ctatcaacat
     2761 gcctaatggt gatgtaaata actatgcaaa agtggatgat gtagttatag atgatgtaaa
     2821 agatggaact tttgttaact acggaaaaat aactaactta aaggttaatg ataaaaatgg
     2881 agccaagata gaaaataatt ctaaaggtga gattggtagt ttaacagtag catctggagc
     2941 aagtcaagta aaagtgacta atggtggtaa gataactaca gttacaaata attctaaagg
     3001 aactactata gataataaag gtacaataag tagtgttaag ggagataatt ctccaacaat
     3061 aagtggaaat agtccaagtt ctaactcttc aggaggaagt tcatcaagtg gaggttcttc
     3121 acatggagga ggaagttctt catcaaaagt agataaagta gttttgaaaa atacaataac
     3181 agcagcaaat aaattgtata atgaagcaat agagggaaca aatgtaggtg aatataaagt
     3241 agggtcaaaa gcaatatata aaacagcaat agacaaagca caggcaatat tagacaaatc
     3301 tggtgtaaca caaaaagaag taaatgatgc agtaacagca ttaaacacag caacagacac
     3361 atttaaagca ggaaaagtag tagcagtaga taaaacagca ttacaagatg cagtaacagc
     3421 agcaacagca ttacatgcta aagcaacaga aggaacagca gaaggaaact atgctgtagg
     3481 atcaaaagca acgtataaaa cagcaataga tgaagcacag gcaatattag acaaatctga
     3541 tgcaacacag aaagaagtaa atgatgcatt atcagcatta aacacagcaa cagagacatt
     3601 tgaagcagga aaagtagtag cagtagataa aacagcatta caagatgcag taacagcagc
     3661 aacagcatta cataatggag caacagaagg aacagcagaa ggaaactatg ctgtaggatc
     3721 aaaagcaaca tataaaacag caatagatga agcacaggca atat
//
LOCUS       DAMJWP010000275         3748 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.282, whole genome shotgun sequence.
ACCESSION   DAMJWP010000275 DAMJWP010000000
VERSION     DAMJWP010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3748
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.282"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(153..722)
                     /locus_tag="PC700_003363"
     CDS             complement(153..722)
                     /locus_tag="PC700_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HDF2582631.1"
                     /translation="MKLPIALSNKHVHLSQADIDVLFGAGHELTKFKPLSQPGQYACE
                     EKVDLVGPKGTLKGVRVLGPARAHTQVEISLADGFPLGVKAPIKESGDIAGTPGVKLV
                     GPAGEVEMKEGVIVASRHIHMSNEDAAKFGVTDKQIVKVKTSGPRALVFENVLVRASD
                     SFNLEMHVDMEEGNAAGVKNGDLVELIVD"
     gene            complement(794..1402)
                     /locus_tag="PC700_003364"
     CDS             complement(794..1402)
                     /locus_tag="PC700_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2582632.1"
                     /translation="MDSNDKKGLKSTEIIIINGSPRINKNCSFIANKVKAMLEECGLS
                     CKIFNIYNMDIEYCTACGFCEKTGYCKFNDDMKPLYKMFDDSKGTIVISPVHFDSISA
                     KLKTVVDRTQAIYASKYVLNKPSIDRNKKRIGMYIAVGGSKSYDSQFKGGQIVMDFFF
                     KSINTKLLHNIYLNNTDDVSYIDNQEFKDIIYKNLKIYIDSI"
     gene            complement(1410..3173)
                     /locus_tag="PC700_003365"
     CDS             complement(1410..3173)
                     /locus_tag="PC700_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HDF2582633.1"
                     /translation="MTNGSYKMKNLVEISAMVTESTDFFNIKDDIIEKMLEVVHPTKA
                     CVNLFYKNDSKYAYLVCSQTLEYVPQIFPINSLKGAKIDFDTYPEYIHEAVNEKKIIY
                     VENVFEDSRAEGERDLAKAEGYIGRIVFPFIINNVVVGFMTCFLKEDDYITEQDIDFI
                     SSVASLLSLSIEITNKNNNVQILIDKLRGSISAINEATKKLYLNKSIDGFLENLSKQA
                     CNITNSKEALIIINSHHNKDTIVSCYNVEQKKKTNLYPMIDMFLKKESLGGYLNNLKP
                     CSKKGINLESYIYYKLQDKNETIGCIVCANSKNYTSDDLNILSILSKQVSVGMQLYEH
                     NEKEVKHKVLENELNILNKQQQLIMDKGKMDCNDEKELYFYHKPARVVGGDFYYATKV
                     DDEHIVYIVADVMGHGMVSNYVVAMIKGAFKVLCNQYNTPREIMTNLNRMLYDEFDKM
                     GVFTTCLIGIIDTKENMITVSNAGHYSPVVVKKDGTIENNLNCKKGIPIGIMKDATYE
                     NNTLSMEDYAMVCMYTDGILEIKNSSKEEYGINRLENFLKENFRLNQQLIVENLKLDL
                     KNFSSKDNYDDDILIVMLKDR"
     gene            complement(3216..3683)
                     /locus_tag="PC700_003366"
     CDS             complement(3216..3683)
                     /locus_tag="PC700_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582634.1"
                     /translation="MKMLNGIIKLFYSEIVKTKINNLDSSGYSLRDECKEIRNITLKI
                     DSVFFKLLKLAFGSILLMIAFNLNMILGIGVFLIGVVYILYKRNMEEQVKEHINNVKN
                     NIDISKITFMDEKGRTGLNALITLLLIGLLTGFNWILVASFVVVFVFTIKNVC"
BASE COUNT         1219 a          666 c          339 g         1524 t
ORIGIN      
        1 tcatattttt gtaagttttt attacatttt ttataatacc cctttatttt ataaattaat
       61 ctaatcgatt aaatttatcc tatagaaatt tattacattt aaaaatatgc tagcaaataa
      121 aatatctact agcataaagt ctatttataa tattaatcaa ctattaattc tactaaatct
      181 ccatttttaa cgcctgctgc atttccctct tccatatcaa catgcatttc tagattaaag
      241 ctatcactag ctcttactaa tacattttca aatactaatg ctcttggtcc agatgtttta
      301 acttttacta tttgcttatc agttacacca aatttagcag catcttcatt gctcatatgt
      361 atatgtctag aagcaactat aactccttct ttcatttcaa cttctccagc aggtcctact
      421 aatttaacac ctggagttcc agctatatca ccagattctt ttattggtgc ttttactcct
      481 aatgggaaac catctgctaa agatatttca acttgagtat gagctctagc tggtcctagt
      541 actctaactc cctttaaagt tccttttggt cctactaaat ccactttttc ttcacaggca
      601 tattgccctg gttgagataa tggtttaaat ttagttaact catgacccgc cccaaataat
      661 acgtctatat cagcttgact caaatgaaca tgtttattag ataatgctat tggtaatttc
      721 attgaaattc cctcctttta attatttaaa ttattgcaat acaattatac ataatattac
      781 ctacaaaatc aaattatatt gaatcaatat atatctttaa atttttatat attatatctt
      841 taaattcttg attatctata taagatacat catctgtatt gtttaaatat atattgtgta
      901 aaagtttagt atttatactc ttaaagaaaa aatccataac aatctgtcca cctttaaact
      961 gtgaatcata tgatttactc ccccccactg caatatacat tcctatccgt ttcttatttc
     1021 tatcaattga aggtttattt aatacatatt tacttgcata tatagcttga gttctatcaa
     1081 caacagtctt taatttagct gatattgaat caaaatgtac aggacttatt actatagttc
     1141 cttttgaatc atcaaacatt ttatataatg gcttcatatc atcattaaac ttgcaatacc
     1201 ctgttttttc acaaaatcca catgctgtgc aatattcaat atccatattg tagatattaa
     1261 atattttaca acttaaccca cactcttcta gcatagcttt tactttattt gctataaaac
     1321 tgcaattttt attaattctt ggacttccat ttattattat aatctctgta cttttaagtc
     1381 cttttttatc gtttgaatcc ataatcaatc tacctatcct ttaacataac tattaaaata
     1441 tcatcatcat aattatcttt tgatgagaaa ttctttaaat ccaattttag attttctact
     1501 attaactgtt gatttaatct aaaattttct tttagaaagt tttctaatct gtttatacca
     1561 tactcttctt ttgatgaatt ttttatctct aaaattccat cagtgtacat acataccatt
     1621 gcataatctt ccattgaaag agtattattt tcataagtag catctttcat tattccaata
     1681 ggtatgccct ttttacaatt taaattattt tctattgtgc catctttctt tacaactaca
     1741 ggactatagt gacctgcatt agacacagta atcatatttt ctttagtatc tataatacct
     1801 atcaaacaag ttgtaaatac tcccatcttg tcaaattcat catataacat cctatttaaa
     1861 tttgtcatta tttctcttgg tgtattgtat tgattgcata atactttaaa agcacctttt
     1921 atcattgcaa caacataatt tgataccatt ccatgtccca ttacatcagc aacaatataa
     1981 actatatgtt cgtcatccac ttttgtagca taataaaaat ctccacctac tacccttgct
     2041 ggtttatggt aaaaataaag ttctttctca tcattacaat ccattttacc tttatccata
     2101 ataagctgtt gttgtttatt taatatgttc aactcatttt caagaacttt gtgttttact
     2161 tccttttcat tatgttcata taattgcatc ccaacagaaa cttgtttgga caatatacta
     2221 agaatattta aatcatcact agtatagttt ttactatttg cacaaactat acatcctata
     2281 gtctcatttt tatcctgaag tttgtaatat atataacttt ccaaatttat gccctttttt
     2341 gagcatggtt ttaaattatt taggtatccc cctaatgact ctttctttaa aaacatatca
     2401 atcataggat ataaatttgt tttctttttt tgctcaacat tataacaact aactatagta
     2461 tccttattat gatgactatt tataattatt aaagcttctt tactattagt aatattacac
     2521 gcttgtttac ttaaattttc caaaaagcca tcaatactct tattaaggta tagtttttta
     2581 gtagcttcat ttatggcact tattgagcct cttagtttat caattaaaat ttgtacatta
     2641 ttatttttat tagttatttc tattgataaa cttaagagtg atgcaacaga tgatataaaa
     2701 tctatatctt gttctgttat ataatcatcc tcttttaaaa aacaagtcat aaatccaaca
     2761 actacattat ttattataaa aggaaaaact attctcccaa tataaccttc agcttttgct
     2821 aaatctcgtt caccttctgc tctagaatct tcaaatacat tttccacata tattatcttt
     2881 ttttcattta cagcctcatg tatatactct ggatatgtgt caaaatcaat ttttgcacct
     2941 ttaagactat ttattggaaa aatttgaggt acatattcta aggtttgcga acatactaag
     3001 taggcatatt tagaatcatt tttataaaat agatttacac atgcctttgt agggtgaaca
     3061 acttctaaca ttttttctat tatgtcatct tttatattaa aaaaatctgt agattccgtc
     3121 accatagcgg atatctccac taaattcttc attttataac tgccattagt catactcaat
     3181 ttctccccct atatattaaa ctatttatta gtattttaac atacattctt tatagtaaaa
     3241 acaaaaacaa cgacaaaact agcaactaat atccagttaa atcctgtcaa taatcctata
     3301 agaagtaatg taatcaatgc atttagacct gttctccctt tttcatccat aaaagttatt
     3361 ttagagatgt cgatattatt tttcacatta tttatatgct cttttacttg ttcttccata
     3421 tttcttttat atagtatata aactacacca attaaaaaca ctcctatacc tagaatcatg
     3481 tttaaattaa aagctatcat aagcagtatt gaaccaaaag ccaactttaa aagcttaaaa
     3541 aatacgctat ctatctttag agttatattt ctgatttctt tacattcatc tcttaaagaa
     3601 tatccacttg agtctaggtt attaatcttt gtttttacaa tttcagaata aaaaagcttt
     3661 attatcccat ttaacatttt catcactcct aaatatttta catatatttg taagttttac
     3721 aattataata ttttttatac ttggtaat
//
LOCUS       DAMJWP010000276         3723 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.283, whole genome shotgun sequence.
ACCESSION   DAMJWP010000276 DAMJWP010000000
VERSION     DAMJWP010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3723
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.283"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            260..1636
                     /locus_tag="PC700_003367"
     CDS             260..1636
                     /locus_tag="PC700_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HDF2582635.1"
                     /translation="MKTIVRRLILYADGMRPTQIMVSGFAAIIVIGALLLTLPIASQS
                     GESIGLLNALFTATSAVCVTGLVMVDTATYWSLFGQIVIITLIQIGGLGFMTVATMFS
                     LMARKKIQLRERLLIQESLNQADLSGLVRLTRFVLIITITIEGIGALVLSTVFIPQFG
                     LSKGIWYSVFHAISAFCNAGFDLMGSVSGPFTSLNSYVNNFTVSMTVCALIVLGGLGF
                     PVVLDIVRKRRFSKLNVHSKVVLFSTATLIFVGALFIFLIEFNQKATMADLPLKGKVL
                     SAIFQSVTARTAGFNTLDLATLRESSVFVMIILMFIGASPASTGGGIKTTTLAVLIIT
                     VRSFLSGKSDIEAFERRLAPSTIKKSLGIFVISISAVIFGTLIISITQPNFTLVQSAF
                     EVTSALATVGSSLAGTPNLNALGKIIIIIFMFMGRVGSLTLFMAILSGGRRKSQPIRY
                     AEGKIMVG"
     gene            1947..2618
                     /locus_tag="PC700_003368"
     CDS             1947..2618
                     /locus_tag="PC700_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HDF2582636.1"
                     /translation="MKQYIVIGCGRFGSSVASTMHLLGHQVMAIDKNEDSVQSISDKV
                     THSLIVDVTDEQALRSLGLGNFDVAVVAIGSDIRASIMATLIAKEMGVELIICKAKDE
                     LQAKVLYKIGADRVVFPERDMGVRVAHNLVSDNILDHIELDPEYSIVEIVTPNSWVGK
                     TLIELELRARYEITVLAIKTGKNINVTPSPDEELTAGSILVIIGQNTSITAITSGNKG
                     IIRRR"
     gene            2809..3606
                     /locus_tag="PC700_003369"
     CDS             2809..3606
                     /locus_tag="PC700_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HDF2582637.1"
                     /translation="MITNINSKDNEKLKYTRALLKSKNRNKESKFIIEGYRIVMLALE
                     CMANLDYVFINEEFENKKEHVKLLEDLDKKNTKIYKTTNKNFKELVDTENTQGIIGVV
                     SFKKKKLSESINKKDKFVLILDRIQDPGNMGTIIRTADSAGVDAIIALKGCVDIYNPK
                     VIRSTMGSIFDMNIIDASQDETVDMLKSLDFNIVSSYLNTENFYDKIDYGSKVALVIG
                     NEANGINEELVSKSDILVKIPIYGKAESLNAAISSAILMYEIKKYLI"
BASE COUNT         1419 a          408 c          682 g         1214 t
ORIGIN      
        1 tttaagtaca aaatagtaaa agctatgata gttttagacc cattatatgt aaaaaatata
       61 gatgtttttg gtgcaattta aattaaaaga tatatataaa aatttaaaat attccaaaca
      121 gaaattataa aaattactat cattgtcttt atgcaatatt tatactataa tagaataata
      181 atttagagga aagctctaaa aataaacaaa ttaagggtct taacagtagt gattttatgt
      241 taaggaggta taaagaaagt tgaaaacaat agttagaaga cttattctct acgcagatgg
      301 aatgcgtcct actcaaataa tggtatctgg gtttgctgcg attattgtaa tcggagcatt
      361 gttgttaaca ctcccgatag cgtcgcagag tggagagagt ataggcttat taaatgctct
      421 atttacggct acttcagcgg tttgtgttac tggtctagtt atggtagata cagcaactta
      481 ttggagttta tttggacaga tagttataat aacattgata caaattggtg gtcttggatt
      541 tatgaccgta gccacaatgt tttcacttat ggcacgaaaa aaaatacaac taagagaaag
      601 attattaata caagagtcat taaatcaagc tgatttatcg ggacttgtaa gacttacaag
      661 atttgtatta ataataacca taacaataga gggaataggt gcactggttt tatcaactgt
      721 ctttattcca caatttggtt tgtctaaagg gatatggtat agtgtatttc acgcaatatc
      781 agcgttttgt aatgccggat ttgacttgat gggcagtgta agtggcccat ttacatcgtt
      841 aaattcttat gtaaacaact ttacagtatc aatgactgtt tgcgcactta tagtgcttgg
      901 agggctagga ttccctgttg tacttgatat tgtaagaaaa agaagatttt caaaattaaa
      961 tgtgcattct aaggttgtat tgttttcaac tgctacactt atttttgtgg gtgcattatt
     1021 tatttttctt atagaattta atcaaaaagc tactatggcg gacttgcctt taaagggaaa
     1081 agtactatca gcaatatttc aatcagtaac agcaagaact gctggtttta atacacttga
     1141 tttagctact ttacgagaaa gtagtgtatt tgtaatgata attttgatgt ttattggagc
     1201 atcacctgca tcaactggtg ggggtataaa aacaacaaca ctagctgttt taatcattac
     1261 agttagaagt tttctatcag gaaaatcaga tatagaagct tttgaaagaa gattagctcc
     1321 atcaactata aaaaaatctc ttggtatatt tgtaatcagt atatcagcag ttatttttgg
     1381 aacattgatt atttcaataa ctcagcctaa ttttacatta gttcaatcag cttttgaggt
     1441 tacatcagca cttgctacag ttggttcaag tttagctgga actccaaatt taaatgcttt
     1501 aggtaagatt ataataataa tctttatgtt tatgggtaga gttggttctc ttactttatt
     1561 tatggctata ctttctggtg gtagaagaaa gagtcaacca atcagatatg cagaaggtaa
     1621 aattatggtt ggttaagtta ccaaggagta atataaattt ataatggatt tagagtagca
     1681 ctgtaaatct gttgataaaa taaatgagcc tagatgtaat tatatgaaga aataaaatca
     1741 actaataaac aaaattaatt aatgaaataa aaaaacaggg aaataaaata caaataaaaa
     1801 ggtaaatcaa aattaaatta atctgtgtat ataaaattat atattgtgta taagttttgt
     1861 atgtgtaaag tatataagtt ttgtatgtgt aaacaattaa tttaagagat attatataaa
     1921 ttgtgtatta gggaggaagt ttcgagatga agcaatatat agtcattggg tgtgggagat
     1981 ttggaagttc agttgcgtct actatgcatc ttttaggaca tcaagtaatg gcaatagaca
     2041 aaaatgaaga ttcagttcaa agtatatctg acaaggtaac ccattcactt atagtggatg
     2101 ttactgatga gcaagcgtta aggtcattag gtttaggtaa ctttgatgta gcagtagttg
     2161 caataggttc tgatataagg gcatctataa tggcgactct tatagccaaa gaaatgggtg
     2221 tagagttgat aatatgtaag gcaaaggatg aattacaagc taaagtgctt tataaaattg
     2281 gcgcagatag agttgtattt ccagaaagag atatgggagt aagagttgca cacaatttag
     2341 tttcggataa tatattagac catattgaac ttgacccaga gtattcaatt gttgaaatcg
     2401 taactccaaa tagttgggtt ggcaagacac ttatagagct tgaattaaga gctagatatg
     2461 agataactgt acttgctata aaaacaggta aaaatataaa tgttacacct tctccagatg
     2521 aggaacttac agccggaagt atcctagtta taatcggtca aaatactagt ataacagcga
     2581 taacatctgg aaataagggg ataattagaa gaagataatt tactatttaa tatatattta
     2641 attgcaatga aagtaaagag tatcatataa ttatgaatag ttatatgata ctatttttat
     2701 ttaatcgaag gtagtattta tttgtaagat tagataaaga gaagttaaat taaaagtaag
     2761 gaggctgtgc taatataaaa atttataagt tattagcatt tagataaaat gattacaaat
     2821 ataaacagta aggataatga aaagttaaag tatacaagag cactattaaa atcaaagaat
     2881 aggaataaag agtcaaagtt cataatagaa ggatacagaa tagtaatgct tgcacttgaa
     2941 tgtatggcaa accttgatta tgtatttatc aatgaagaat ttgaaaataa gaaagaacat
     3001 gtaaaactat tagaagattt ggataaaaaa aacacaaaga tatacaagac tactaataaa
     3061 aactttaaag aattagtgga tacagaaaat actcaaggaa taataggtgt agtttcattt
     3121 aagaaaaaaa aattaagtga aagtataaat aaaaaagata aatttgtatt gattttagat
     3181 agaatacaag acccaggaaa tatggggact ataataagga ctgctgattc tgctggagta
     3241 gatgccataa tagcactaaa gggatgtgtc gatatataca acccaaaagt aattaggtct
     3301 actatgggtt ctatttttga tatgaatata attgatgctt cacaagatga aactgtggac
     3361 atgcttaaat cattggattt taatatagtt tcaagttact taaatacaga aaatttttat
     3421 gacaaaatag attatggttc aaaagtagca ttggtgatag gaaacgaagc aaatggaata
     3481 aatgaagaac ttgtatcaaa gtctgatatt ttggttaaga tacctatata tggtaaagcc
     3541 gagtcgttaa atgctgcgat aagctctgct atactgatgt atgaaataaa aaaatactta
     3601 atttaatgta ttgtaataaa tataatgtca tgttataatc ttgaattaaa tagtagagta
     3661 tcaaaaatag tcaatatata ttaaaaaaat aattaaatta attatatatt agatgtatat
     3721 cat
//
LOCUS       DAMJWP010000277         3706 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.284, whole genome shotgun sequence.
ACCESSION   DAMJWP010000277 DAMJWP010000000
VERSION     DAMJWP010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3706
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.284"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     misc_binding    295..533
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            597..2516
                     /gene="thrS"
                     /locus_tag="PC700_003370"
     CDS             597..2516
                     /gene="thrS"
                     /locus_tag="PC700_003370"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDF2582638.1"
                     /translation="MIKVALKDGSIKEFENAISVMDVAKSISEGLARNVVAASVNGEV
                     VGLDHIIDTDCDLNLFKFEDKEGKEVFRHTSAHILAQAIKRLYPEAKLAIGPSIENGF
                     YYDIDLDHRLVPEDLEKIEAEMKKIAKEDLKIERFELPRNEALELMKEQGEDYKVELI
                     SDLPESEIISFYKQGDFTDLCRGPHLPSTKKVKAVKLQSVAGAYWRGDENNKMLQRIY
                     GTSFEKNKDLEEYLHLLEEAKKRDHRKLGKELGLFMIPEEGPGFPMFLPKGMELKNEL
                     LKFWREIHRKAGYIEIESPIILNRKLWETSGHWYHYKENMYTVKIDDEDYAIKPMNCP
                     GGLIYYNSQLHSYRDFPMRVAELGRVHRHELSGALQGLMRVRAFTQDDSHIFMLPEQI
                     KDEIKGVANLIDGIYKTFGFEYNLELSTRPENSMGSDEEWEAAENGLREALEELGLPY
                     TINEGDGAFYGPKIDFHLKDCLGRTWQCGTIQLDMQLPRQFDNTYIGQDGEKHRPVMI
                     HRVAFGSIERFIGILIEHYAGKFPVWLSPTQVKILPISDKFMDYANEVKKELFDKGIR
                     VELDDRAEKIGFKIREAQLEKVPYMLIVGEKEVADNNVSVRSRDKGEIGSIKLDEFIA
                     SISKEIESRESIIQD"
     gene            3284..>3706
                     /locus_tag="PC700_003371"
     CDS             3284..>3706
                     /locus_tag="PC700_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582639.1"
                     /translation="MIEKVEEKSKIIKTSKEYKRTIIEEKDKEWTKKLNDILSRSSFC
                     NDELDKIKALIPKEILTNENVGEVVTEDGYAKPEYDEVFKSLFTLNNEYDLLVNFIND
                     ILKDAPYANRNIKPFTQIKRIIRVETDPTINYIGEKRPR"
BASE COUNT         1447 a          390 c          683 g         1186 t
ORIGIN      
        1 tgattgtatt taagtataca aaacctaaaa agagtacaaa aattagaagt tagttttaat
       61 aaaaattagt tttagtattt atctagtcat atctatcatg catgtggata cgttttaaaa
      121 attttaaatt tatgaaaata ttataatatc actataaatc aagagattaa tttaatataa
      181 aaatataaga atattggttt acagctgatt gagatgaaaa ataatgggta attaatatat
      241 tacattaatg ttgattgatg tataactatc tgttataatg aaaatatatt aaaaatcata
      301 gtgttgaaga ggaagagtag agtagaaacc attctcagag aatttgggtc tatggctgag
      361 agcccatgta ataggtctat ttgaaaacta ccttggagct gagcttatta gcagtatttg
      421 tatttgtact atacgtgaaa gtgctcgggt gacgacctta tagtcatcaa gagggttgta
      481 tatacttgta tacgccaact tgggtggaac cgcgaattaa tatcgtccca aacatatttg
      541 gggcgatttt ttattacaaa aaatctaaaa attatttcaa taaggaggaa aaaacaatga
      601 ttaaagttgc tttaaaagat ggctcaataa aagaatttga aaatgctatt tctgttatgg
      661 atgtagctaa atccattagt gaaggattag ctagaaatgt ggtagcagcg tctgtgaacg
      721 gagaggttgt aggtctggac cacattatag atactgattg tgacttgaat ttatttaagt
      781 ttgaagataa agaaggtaag gaagttttta gacatacatc agcacatata ttagctcaag
      841 ctataaaaag actttaccca gaagcaaagc ttgctatagg accaagtata gaaaatggat
      901 tctactatga tatagattta gaccatagat tagttccaga ggacctagag aaaatagaag
      961 ctgaaatgaa aaaaatagct aaagaagatt taaaaataga aagatttgaa cttcctagaa
     1021 atgaagcttt agagcttatg aaagaacaag gagaagacta taaggtagaa cttatatctg
     1081 atttacctga aagtgaaata atatctttct acaagcaagg tgattttact gatttatgta
     1141 gaggaccaca tttaccatct actaaaaagg taaaagctgt taaattacaa agtgtagcag
     1201 gtgcatattg gcgtggtgac gaaaataata agatgcttca aagaatatat ggaacttctt
     1261 ttgagaaaaa caaagactta gaagagtact tacatttact agaagaagct aaaaagagag
     1321 accacagaaa attaggtaaa gaattaggat tatttatgat acctgaagaa ggtccaggat
     1381 tccctatgtt tttaccaaaa ggtatggaac ttaaaaatga attattaaaa ttctggagag
     1441 aaatacacag aaaagctgga tatattgaga tagaatctcc aatcatatta aatagaaaat
     1501 tatgggagac ttcaggacac tggtatcatt acaaagaaaa tatgtataca gttaaaatag
     1561 atgatgagga ttatgctata aaacctatga actgtcctgg tggattaatt tactataact
     1621 ctcaactaca ttcatataga gatttcccaa tgagagttgc agagcttggt agagttcata
     1681 gacatgagtt atctggtgca ttacaaggat taatgagagt tagagcattt actcaagacg
     1741 attcacatat attcatgtta ccagagcaaa ttaaggacga aataaaagga gtagctaact
     1801 taatagatgg tatctacaaa acttttggat ttgaatataa tttagaatta tctacaagac
     1861 cagagaactc tatgggaagc gacgaagagt gggaagcagc agaaaatgga ttaagagaag
     1921 ctttagaaga gttaggtcta ccatatacta ttaatgaggg tgatggagca ttctatggtc
     1981 ctaagataga cttccatcta aaagattgtt taggaagaac ttggcaatgt ggtactatcc
     2041 aattagatat gcaacttcca agacaatttg ataatactta tattggtcaa gatggtgaaa
     2101 agcatagacc agttatgatt catagggttg cttttggtag tatagaaaga tttataggta
     2161 tattaataga gcattatgct ggtaaattcc cagtttggtt atctccaact caagttaaga
     2221 tacttcctat atctgataaa tttatggatt atgcaaatga agttaagaaa gagttatttg
     2281 ataagggtat aagagttgaa ttagatgata gagctgaaaa aattggattt aagataagag
     2341 aagctcagct tgaaaaagtg ccatacatgt taatagtagg tgaaaaagaa gtagctgata
     2401 ataatgtttc tgttagatca agagataaag gtgaaatagg tagtattaaa ttagatgaat
     2461 ttatagctag tatttcaaaa gaaattgaat caagagaaag tattattcaa gactaatata
     2521 taatatatta gtagctaaga tattgttata taagataagt ttaatcaata tgttgatatg
     2581 ttgatacata gtgattaaat ttatgaaaag atgagtataa ttagaggtac agttaatcat
     2641 atttttatga ttaatgtacc tctagttttt tgtatggtag atttattaat aaatatatca
     2701 tagaagtgtt ttgaacatgg aaatatttat gtgcattctt taaataaaag tttatttttt
     2761 gaatgataat taagtgcttg ttggtaaggt tatggctgta aaaatcataa ctttattttt
     2821 atatttgttc cattttcata aaaattatta tccctttaag aaaatttctt aatcctcttg
     2881 aaatatttcc tttttagtct aaagttaaat ttctttcttc aaaatgaata tttaaatgaa
     2941 ctaaaattta agtacatttt ctaatcttat gagtctattt ttcatagata attgccctat
     3001 aatagaagaa tctatgttgg ggctatgtgg tgacaataac agcattgtca gtgattaatt
     3061 aatgctttat aaatgattaa ttagtgtttt atgttatttt tggacttacc ctctatacat
     3121 agattaattt atttaacttt agatatagag agaatgtcaa gtacatacac agacattaga
     3181 gtttaagaaa gtgtttatgg agcaagaact tttaaaagaa gtagtgactt agtttaattt
     3241 caagaaatga taaatacaaa agaggaaaaa gagaggtgtt gaaatgatag aaaaagtaga
     3301 agaaaaatct aagattatta aaacatcaaa agaatataag cgaactataa ttgaagaaaa
     3361 agataaagaa tggaccaaaa aattaaatga tatacttagt agaagtagtt tttgtaatga
     3421 tgaattagat aaaatcaagg cgttaattcc aaaagagatt ttgactaatg aaaatgttgg
     3481 agaagttgtg actgaagatg gatatgctaa gcccgagtat gatgaggtat ttaaatcatt
     3541 gttcacttta aataatgaat atgatttact tgtaaatttt ataaatgata ttctaaaaga
     3601 tgctccatat gctaacagga atatcaaacc gttcactcaa ataaagagaa taattagagt
     3661 tgaaactgat ccaacaatta attacattgg agagaaaaga cctaga
//
LOCUS       DAMJWP010000278         3642 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.285, whole genome shotgun sequence.
ACCESSION   DAMJWP010000278 DAMJWP010000000
VERSION     DAMJWP010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.285"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(155..1426)
                     /gene="serS"
                     /locus_tag="PC700_003372"
     CDS             complement(155..1426)
                     /gene="serS"
                     /locus_tag="PC700_003372"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDF2582640.1"
                     /translation="MLDIKRIRENLDDIKKAMERRGEREFDLDAVVELDNKRREILQE
                     VEVMKNELNVNSKKIPQLIKEGKDVTEEKTRLKELSDKIKGIDEKVKEVEAKMEYTLM
                     RIPNVPHPEVPQGETDEDNVQIRTWGEPTKFDFEYKAHWDIGTGLGILDFERAGKITG
                     SRFTLYRGLGARLERSLMNFFLNTHTAKHGYTEVLPPFMANRNSFIGTGQLPKFEEDM
                     FKIEGLEYFLIPTAEVPVTNIHANEILDVAELPIKYCAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVELVKFVKPEDSYNELESLTHDAETMLQMLGLPYRVVKICTGDLGFTAAFK
                     YDLEVWMPSYNRYVEISSCSNFEDFQARRAGIRFKRDKKSKAEYVHTLNGSGLAIGRC
                     LAAILENYQQADGSVVVPEELRPYMGVDVIK"
     misc_binding    complement(1551..1757)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1884..2765)
                     /locus_tag="PC700_003373"
     CDS             complement(1884..2765)
                     /locus_tag="PC700_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HDF2582641.1"
                     /translation="MNFLSLAKAASTNSNITKRTLENMLNNFSDNIPNIIYAIIVFVI
                     GIYISKIVRKMVSRFLIKYGMSKGVNNFIVYGIYISMLSIISLISLGVIGIQTTSVVA
                     VLGAAGFSIGLAFKEILSNLGSGMIILFFKPFNIGDYIQGSGVEGTVSDIQIFSTVLK
                     TPDNKTIIIPNFQLTSNNIINYTHQNKRRIDFSYNISYDSDIDVVKSILNEIFINEKR
                     ILDDPKPIIGLNSIGNNTMQIVARPWVKTDDYWDVYFDVMEKVKNKFDENNIKVPFVP
                     SSLLFNNMSNSNTMDKR"
     gene            complement(2777..3286)
                     /locus_tag="PC700_003374"
     CDS             complement(2777..3286)
                     /locus_tag="PC700_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HDF2582642.1"
                     /translation="MNSDERRSKLIDILKESKCPVKGGMLAEILNVSRQVIVQDIALI
                     RARGFEIIATPQGYIIYNQPYFTKQIKCKNHRNSKEIYDELKIIVDLGGIVKDVIVNH
                     PTYGQITAELNIHSKMDIDNFMKKVETNEFKQLSVLTECSHIHTIETLKEETIEVITK
                     ELEDRGILS"
     gene            complement(3413..3529)
                     /gene="rrf"
                     /locus_tag="PC700_003375"
     rRNA            complement(3413..3529)
                     /gene="rrf"
                     /locus_tag="PC700_003375"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1163 a          643 c          367 g         1469 t
ORIGIN      
        1 ttagtgtttt atgatgaaaa ttctgctgtg taaattaaca taaaaaaact atgaaattaa
       61 tatttataat aatttcatag tttttttatt tattgtttac tcataatata ttctcttcat
      121 caaagcatac tctacatata caattaatat aaatttattt tataacatca acacccatat
      181 aaggtcttaa ttcttctgga actactacag aaccatctgc ctgctgataa ttttctaata
      241 tagcagccaa acatcttcct atcgctaatc cagaaccatt taatgtatga acatattctg
      301 cttttgattt tttatctctt ttaaatctta ttcctgctct tcttgcttgg aaatcttcaa
      361 aatttgaaca agaagatatt tctacatatc tattgtaact tggcatccaa acttctaaat
      421 catatttaaa tgcagctgta aatcccaaat ctcctgtaca tatttttaca actctatatg
      481 gaagacctaa catttgtaac atagtttcag catcatgagt taatgattct aattcattat
      541 aagaatcttc tggtttaaca aatttaacta attcaacttt attaaattga tgttgtctaa
      601 ctaaacctct tgtatctctt cctgctgaac ctgcttcaga tctaaaacaa ggagtatatg
      661 cacagtattt aattggaagc tctgcaacat ctaatatttc attagcatgt atattagtaa
      721 caggaacttc agctgttggt attaagaaat actctaatcc ttctatttta aacatatctt
      781 cttcaaattt aggaagttga ccagtcccta taaaactatt tctattggcc ataaatggag
      841 gtaagacttc tgtatagcca tgcttagctg tatgagtatt taaaaagaaa ttcattaaag
      901 atctttctaa tctagctcct aatcccctat ataaagtaaa ccttgaccca gttattttcc
      961 ctgctctttc aaaatctaaa atacctagac ctgtccctat atcccaatgc gctttatact
     1021 caaaatcaaa tttagtaggt tctccccaag ttcttatttg aacattgtct tcatctgttt
     1081 caccttgtgg tacctctgga tgaggaacat ttggtattct cattaatgta tattccattt
     1141 ttgcttctac ttcttttact ttctcatcaa taccttttat cttatctgat aactctttca
     1201 atcttgtctt ttcttctgtt acatcttttc cttcttttat taattgaggt atttttttag
     1261 aatttacatt taactcattt ttcataactt caacttcttg aagtatttct cttcttttat
     1321 tatctagttc cactacagca tcaaggtcaa attctctttc acctcttctt tccatagctt
     1381 ttttgatatc atctagattt tctcttattc ttttaatatc taacatatta attcctccta
     1441 ataattttga taatataaat tataattcca attaatttac aagtattcta gctgtaaact
     1501 tttaatttaa acaaatttga aaaataaaaa aagacctccg tttaccctat aaaagggacg
     1561 aaaatctttt atccgcgttg ccaccctaat tgattatact aaaacgtata atcctctcag
     1621 acctataacg taggtaaacc gtctagcttt aactagaagc tctagagtag attcaaaaac
     1681 cattgtaatg agttctcacc atccactcat tctctgaata caatctgttt tttactattt
     1741 tccttcaccg ctttttaact aattgtttta tttgataatc attatattat atatattaac
     1801 atctttcaat actattattc aaattttttt agaattttct ttaatttttt atattaaatt
     1861 cactttttta cttaattgaa attctatctt ttatccattg tatttgaatt gctcatatta
     1921 ttaaataata acgaggaagg aacaaatgga acttttatat tattttcgtc aaatttattt
     1981 ttgacttttt ccattacatc aaagtaaaca tcccagtaat catcagtttt aacccatggt
     2041 ctagcaacta tttgcatagt gttgtttcct atagagttta aacctataat tggttttggg
     2101 tcatccaata tccttttttc atttataaaa atttcattca atatggactt aactacatca
     2161 atatcactat catatgaaat attatatgaa aaatctatcc ttcttttatt ttgatgtgtg
     2221 taattaatta tattgttact tgtcaattga aagtttggta taattatggt tttattatct
     2281 ggggttttaa gaacagtact aaatatttgt atatcagaaa ctgtgccttc tacacctgag
     2341 ccttgaatat aatcacctat attaaatggc ttaaaaaata aaataatcat acctgaacct
     2401 aaatttgata atatttcttt aaatgctaaa cctatactaa atccagcagc tcctaataca
     2461 gctacaactg atgtagtttg tatccctata acacccaagc ttatcaatga tataatagat
     2521 aacatagata tgtatattcc atatacaatg aaattattta cacctttact cattccatat
     2581 ttgattaaaa accttgaaac catctttctt actatcttag atatatagat acctattaca
     2641 aatacgatta ttgcatatat aatatttggt atattatcac taaagttatt aagcatattt
     2701 tctaaagttc ttttagttat attactattt gttgaagcag ccttagctaa tgataaaaaa
     2761 ttcatttaac tcctccttat gataatattc ctctgtcctc taattctttt gttataactt
     2821 ctatagtctc ttcttttaat gtctcaattg tatgaatatg actacattca gttagaactg
     2881 ataactgttt aaattcattt gtctcaacct tcttcataaa attatctata tccattttag
     2941 agtgaatatt aagctctgca gttatctgac catatgttgg atgatttacg atgacatcct
     3001 taacaattcc acctaaatct acaataatct ttaattcatc ataaatttct ttagaattcc
     3061 tgtgattctt acattttatt tgctttgtaa aatatggttg attatatatt atatatcctt
     3121 gaggtgtagc tattatttca aatcctctag ctcttataag ggctatatct tgtactatta
     3181 cttgcctact tacatttaat atctcagcta gcattccacc ttttacaggg cacttagatt
     3241 cttttaaaat atctattaat ttgcttcttc tttcatcaga attcatatta caccttcttt
     3301 ctaaatcaaa aactttacct atactatata attctatatt ttatcttatt ataaatcaat
     3361 attgatttta tattctattt aatattaaac tttattgcaa ataaaaaaga ttacgtggct
     3421 acgtcctact ctcccaggca gtttcccacc aagtaccatc agcgctagag agcttaactt
     3481 ctgtgttcgg aatgggaaca ggtgtatcct ctctgctatt gtaaccacat aatctttatg
     3541 cttaatattt ctattaagtt tttagaatct agtaatacta gtttctttag agttaacact
     3601 ctaaaaacta catatatatt ttattgaatg gacatcaaat aa
//
LOCUS       DAMJWP010000279         3605 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.286, whole genome shotgun sequence.
ACCESSION   DAMJWP010000279 DAMJWP010000000
VERSION     DAMJWP010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.286"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            159..536
                     /locus_tag="PC700_003376"
     CDS             159..536
                     /locus_tag="PC700_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2582643.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDVTVTSKDVIEAMEQKYGWKQTTT
                     LTLLSRLVKRGFLDAQKIDRYTHYTIRVKHKDYLSFETKDFLSNIHDDSLRSLISALH
                     DDENIGKDKLDFLESYFDNLKDE"
     gene            529..1563
                     /locus_tag="PC700_003377"
     CDS             529..1563
                     /locus_tag="PC700_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HDF2582644.1"
                     /translation="MNNYMLYVFENLIKTTIVCSLGICLLLFLKRYLFKKFSKRFNYY
                     IWLIIVFRMLLFLFNYTIVYEVKEPKENAVGNNITQIDISTDNNLMLYVAYLWLVVTI
                     VIAVYTFIKYTRFKNLVVDVSYDIEDNDVNCIYKNLLKELNIKKKIELRGSDELISPA
                     GMGLFKSYIFLPDYPYSKDELTWILKHELMHFKNKDILIKFLVLSVRIIYWFNPLVYV
                     MSNKVNLDCELCCDESVLTDCSLKDKKEYALALIKSIKLSKNYNSGILTTEFNKTSLE
                     KRLDSIVKKKGKNGILVLLILITLFSVTYFNFESISFDNSNSLRNMGILQKSGVDLKE
                     EDDISMRVIN"
     gene            1629..2216
                     /locus_tag="PC700_003378"
     CDS             1629..2216
                     /locus_tag="PC700_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HDF2582645.1"
                     /translation="MKNLKKILIITGCLTLISTSLVFANTTTDVDTSKDDDSIISSCT
                     ITGYEFTYENAPQSVKFDYEKKCEALNIKPSKNDIIFVNKDSIDANDVENYVEPKAYI
                     IRYKKGYVFEVTGPKSYTLNVSNTVVGYNRVESGNPVHLVQLLCRTLDGSSIDDDSEF
                     GPATYNAVKSLQGRLGLTRDGIVGRSTWDAAGRRL"
     gene            complement(2594..3355)
                     /locus_tag="PC700_003379"
                     /pseudo
     CDS             complement(2594..3355)
                     /locus_tag="PC700_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612258.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1337 a          388 c          518 g         1362 t
ORIGIN      
        1 atttaaatag atatatttta aaatctaact tttaatattg taaaaaaatt agaaatttaa
       61 caaaaagtac aaaaattcac atttttttca taaagtgttt gactaaaaaa ttcacatgtt
      121 gtagaattaa ggtagcagga gttttgaggg aggtgtaaat aatgataaga aaaataccac
      181 aagcagaact aaaagtaatg aaattcattt ggaaagtaga tgttacagtg acatcaaaag
      241 atgttattga agctatggaa caaaaatatg gctggaaaca aactactaca ctgacacttt
      301 tatctaggct ggtaaaaaga ggattcttag atgctcaaaa aatagacaga tatacacatt
      361 acacaataag agtaaaacac aaagattatt taagttttga gacaaaggat ttcctaagta
      421 atatacatga tgactctttg agaagcttaa tttcagcatt acatgatgat gaaaatatag
      481 gtaaagataa gctagatttt ctagaaagtt attttgataa cttaaaggat gaataattat
      541 atgctatatg tttttgaaaa tcttataaag acaactattg tatgtagtct tggtatttgt
      601 ctattattat ttttgaaaag atatttattt aagaagttta gtaagagatt caattattat
      661 atttggttga taatagtctt tagaatgtta ctttttctat ttaattatac aatagtgtat
      721 gaagtaaaag aacccaaaga aaatgcagta ggaaataata ttacacaaat tgatatttct
      781 acagataata atcttatgtt gtatgtagct tatttatggt tagttgtaac tatagtcatt
      841 gcagtttata catttataaa gtatacaaga tttaaaaatc tagttgttga tgtatcttat
      901 gatattgaag ataatgatgt aaactgtatc tacaaaaact tgttgaaaga attaaatata
      961 aaaaagaaaa ttgaattaag aggttctgat gaattgataa gcccagcagg catgggatta
     1021 tttaaatctt acatattctt accagactat ccttatagta aagatgaact aacttggatt
     1081 ttgaaacatg aattaatgca ttttaaaaat aaagatattt taataaagtt tttagtgtta
     1141 tctgttagaa taatatattg gtttaatcca cttgtttatg taatgagcaa taaagtaaat
     1201 ctagattgtg aattatgttg tgatgaaagt gttttgactg attgttcttt aaaggataag
     1261 aaggagtatg cattagcact tataaagtca attaaactta gtaagaatta taatagtggt
     1321 attttaacaa cagagtttaa taagacaagt ctggaaaaga gacttgacag tattgttaag
     1381 aaaaaaggta aaaatggaat attggtgtta ttgattttga ttacattatt ttcagttaca
     1441 tattttaatt ttgaaagtat tagttttgat aattcaaatt ctttaaggaa tatggggatt
     1501 ttacaaaaat caggagtaga tcttaaggaa gaagatgata ttagcatgag agttataaat
     1561 taatagatta atgtatgtaa tgaaaaggta tattgtcgta ttattaataa attaggagga
     1621 attatgaaat gaaaaattta aaaaaaattt taataattac aggttgtcta actctaatat
     1681 ctactagttt ggtatttgca aatactacta ctgatgtaga tacatcaaag gatgatgatt
     1741 caataataag ttcatgtact ataactggtt atgaatttac atatgaaaac gcaccacaat
     1801 ctgtaaaatt tgattatgaa aaaaaatgtg aagcgctaaa tataaaacca tctaaaaatg
     1861 atataatttt tgttaacaaa gattctatag atgcaaatga tgtagaaaac tatgttgagc
     1921 caaaagcata tattattaga tataaaaaag gttatgtttt tgaagttact ggacctaaga
     1981 gttatacatt aaatgtaagt aatactgtag tcggatataa tcgtgttgaa tcaggtaatc
     2041 ctgtgcattt agttcaactt ttatgtagaa ccttagatgg ttcaagtatt gatgatgata
     2101 gtgaatttgg acctgctaca tataatgcag ttaagtcact tcaaggaaga cttggattaa
     2161 caagagatgg aattgttgga agaagtactt gggatgcagc tggaagacgt ctttaaaaag
     2221 aaagctattg cacagataat tttaaattgc tgaatctgta aacatttaca tgtattcttt
     2281 aaataaaaat ttatttttta agtgataatt aaatttttac taatagggct atggctttaa
     2341 aaatcatagc cttattttga tatcttttcc atttttgcaa aagctattaa ccctttaaga
     2401 aaatttcttg tttttttgaa aaatttcttt cttcaaaata aatacttaaa tgagctaaaa
     2461 tttgagcaca atttctaatt ttctgagtcc atttttaaga aatatatatg gttgttaagt
     2521 atagtgattt gtgcaaataa ttatccaaag taaaaataga tttcaattta gtaacttttc
     2581 ttaataatct attctaactt cccattgaat ttgtagtata tatcgatgta tttatttctt
     2641 gagagtactt aaaatatgtt actaattcat tccaattgtt tcaccaacct cttaatacta
     2701 ttgtatattg attgtactat tttttctcta gtgatagctc acttaatgct acctcttctg
     2761 taattgtagc atatctttct ttaaatcttt tacagattct tttctatttt tatatgatac
     2821 ataatttaaa gtatttttaa tttgatgcac aacatatctt tgtacttaag tatcaggaaa
     2881 tatcgcttta attgcattag aaaattcaac tagtctatct atagaagctg ctattaatac
     2941 atctttaatt cccatgtttc tagcatctat aagcactttt agccaatact ttgaaatttc
     3001 attttctcca atccatattc ttaaaacctc ttttatgcta catatagcta tatagactgt
     3061 tttgtttatt attcgacctc acttctaact ttataatgta tagcatctat aaatacaatt
     3121 gaataaatta tattaagagg tctaagttac catttatgag ttaaaggaat aactttatca
     3181 gtagttttag ttattagtga atgagataat atcaattcca taagtacttt taagatgact
     3241 taatatattt tgagtagaca tgccttttga gtatattcca atcacttgat tttttattcc
     3301 taatatatca ttttctatca taacctaaaa gttcattaag ctccgcttct aacatttaat
     3361 ggattgtact ttcaaataaa tcttttagta tatcttgaat atctgtaatt gttttggcat
     3421 catattcttc aataaatttt tttaatattt tagtttttgt taaacataaa aagtgaacat
     3481 cttcctttgt atagtagtga tattttaatt atcacaactt tttagaagaa tacaaacttt
     3541 attttaaaga cactgaaaat ggtgttttta tacctttatt tgctatgtat aattttactt
     3601 ataac
//
LOCUS       DAMJWP010000280         3564 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.287, whole genome shotgun sequence.
ACCESSION   DAMJWP010000280 DAMJWP010000000
VERSION     DAMJWP010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.287"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..653
                     /locus_tag="PC700_003380"
     CDS             <1..653
                     /locus_tag="PC700_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDF2582646.1"
                     /translation="TKIIKVQKPKFTGELEIIENLEDILEDIFNDEKIFNKIIENEVI
                     SDLDSVRVSFESCIFRNVSFENCNLKKIDLLDVVFENCNLSNSFFDGGSIYRVEFKNS
                     KLIGVRFDDCILKNVLFKDVLGKYSNFSFSKFKIVSILDSNFENSVFQEVKGDNLVFE
                     YTNLKKANFNGTKLSGIDFTTNNIEGIEIGIDEIRGAIFDVSQAIDLTKLMGIIIK"
     gene            794..2065
                     /gene="brnQ"
                     /locus_tag="PC700_003381"
     CDS             794..2065
                     /gene="brnQ"
                     /locus_tag="PC700_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDF2582647.1"
                     /translation="MRDIFIVGFALFSMFFGAGNLMFPPFLGMESGKDWLIPLIGFVV
                     ADAGIAMLVIIATARCKGSMDDVLRRAGKGLARVMSVAALACLALLVIPRTCATTYEM
                     GIMPIFGDHGPVAKAIFSIIFFGLTLAFTIRSSKVIDIIGKYLTPALLLVLFVLIVKG
                     IISPAGPMSPEHMIDKNLFGEGISQGYQTMDALGAASMATIMLMSIIAKGYTSDKDQI
                     GMTIKAGFVACVFLAVVYGGLTYLGATVSTLYDTSIPQASLLVEITSILLYGQVGKVI
                     LGVIVALACLTTSTGLTASISTYFEGISKASYKQLVIGICVASMLISNLGVDSIIAIS
                     VPILQTIYPVLLAIVVMELAGKHIKNDNAFKGAAYVTLVISLLSAINGMTGAVPFIQS
                     LPLAGLGFNWVIPAILGGIVGNFIKSNKQVA"
     gene            2267..3454
                     /locus_tag="PC700_003382"
     CDS             2267..3454
                     /locus_tag="PC700_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HDF2582648.1"
                     /translation="MLRKITAFMLSICLIIGLTGCSLLVGKKDKEKIIDDFKVAVVTQ
                     PLSENKVQYNMVEEMTKEYEEENKIDKDKDGQTKVKQTIKHVVLPENFTSNIDSAINK
                     IVKLADDKEVQAIVVSTDQAGLLPALQKVKEKRPEIITISAPMGDDKNQLSQFVDVNL
                     GVSAEERGKVLAERSKEMGAKAFIHYASTDDLKDVNIAKRLEMIKETCKNIGLPFVQV
                     NTPNINTEEDKNKVKQFLNEDIEKQVKKYGKDINVFGVNEYMDEVILTKALELKYIVA
                     EQSNPSPIQTYPSVMGLKISEKDAQNYDKINDMISEKAKAFGMSNRLGGYPMPMDAFL
                     PSVAIYLATEMVKQDLTQEDVCDPDYLEAFTELRFGIGSEFTPLTEVLYNYQSVILSQ
                     LIY"
BASE COUNT         1303 a          398 c          657 g         1206 t
ORIGIN      
        1 tgactaaaat tattaaagtg caaaaaccta aatttacagg agaattagaa attattgaaa
       61 atcttgaaga tatacttgaa gatatattta atgatgaaaa aatatttaat aaaattatag
      121 aaaatgaagt tataagtgat ttagattctg taagagttag ctttgaatct tgtatattta
      181 gaaatgtatc ctttgaaaat tgcaatttaa aaaagattga tttacttgat gtggtatttg
      241 aaaactgcaa tttatcaaat tcattttttg atggaggaag tatatacaga gttgaattta
      301 aaaatagtaa attgatagga gttagatttg atgattgtat acttaaaaac gttttgttca
      361 aggatgtttt aggtaagtat tctaactttt cattctctaa atttaagata gtttctatct
      421 tagattcaaa ttttgaaaat tctgtatttc aagaagtaaa aggtgacaat ctagtatttg
      481 agtatacaaa cttaaaaaaa gcaaacttta atggaactaa actttctggc atagatttta
      541 ccactaataa tattgaggga attgagattg gtatagatga gattagagga gcaatttttg
      601 atgtgtcaca agcaattgat ttaacgaaat tgatgggaat tatcataaaa tgaaaaaaat
      661 attcataaaa taaattggga aaacgttaac aatgtgtgaa tttttaccag attgacattt
      721 ttctgaaaat aaagtaagat tatcttaata ttcaaaatat tttcaaaggg ggtaatttag
      781 tgtgagtaag cagatgagag atatttttat agttggtttt gctttatttt ctatgttctt
      841 tggtgcagga aatcttatgt ttcctccatt tttagggatg gaatccggca aagactggtt
      901 aattcctctt attggatttg ttgttgcaga tgctggtata gcaatgcttg ttattatagc
      961 aacagcaaga tgcaagggaa gcatggatga tgtacttagg agagctggta aagggttagc
     1021 aagagtaatg agtgtagctg ctctagcatg tttagcactt cttgtaatac caagaacttg
     1081 tgcaacaaca tatgaaatgg gtattatgcc tatatttgga gaccatggac cagtagctaa
     1141 agctatattt tcaataatct tctttgggtt aactttagca tttactatta ggtcttctaa
     1201 ggttatagac ataataggta agtatttaac tccagcgtta ttattagtat tatttgtttt
     1261 aatagtaaag ggtataattt ctccagcagg acctatgagt cctgagcata tgatagataa
     1321 aaatctattt ggtgaaggta tatcacaagg ttatcaaaca atggacgctc taggtgcagc
     1381 atctatggca actataatgt tgatgtctat aatagctaaa ggatatacat cagataaaga
     1441 ccaaatagga atgactatta aagctggttt tgtagcctgt gtatttctag cagtagtata
     1501 tggtggtctt acttatttag gtgcaacagt ttcaacttta tatgatacta gtatacctca
     1561 agcatcttta ttagttgaaa taactagtat attgttatat ggtcaagtag gaaaggtaat
     1621 tttaggtgta attgtggcac ttgcctgtct gactacttct acaggtttaa cagcatcaat
     1681 atcaacttat tttgaaggaa taagtaaggc gagttataaa caactggtta taggtatatg
     1741 tgtagctagt atgcttatat caaatttagg tgttgattct ataatagcta tatcagttcc
     1801 aatcctacaa actatatatc ctgttttatt agcaatagtt gttatggagt tggctggtaa
     1861 acatataaag aatgataatg cttttaaagg tgcagcatat gttacattag tgattagtct
     1921 attatcagct ataaatggaa tgacaggagc tgttccattt atacaaagct tgccactcgc
     1981 aggtttgggc tttaactggg taataccagc tatattgggt ggaatagttg gtaactttat
     2041 aaaaagcaac aaacaagtag cataataatt tgtaattaat ttaattttat aatatattaa
     2101 tttttagtac tatgggtagg tttaatctaa acctaatcta cccatagtac tttttagatt
     2161 ggattggaat agcatgtata taatttttta taatataatt tttctttgaa gtatgataaa
     2221 attagtaata gtgataagaa agtacaaatt taggaggttt taattaatgc ttagaaaaat
     2281 aacagcattt atgctatcca tatgtttaat tattggactt acaggttgta gtttacttgt
     2341 aggcaagaaa gataaagaga aaattataga tgattttaaa gttgcagtgg taactcaacc
     2401 attatctgaa aataaagtac aatacaatat ggtggaagag atgacaaaag aatatgagga
     2461 agagaataaa attgataaag acaaagatgg tcaaactaaa gttaaacaaa ctataaaaca
     2521 tgttgtattg ccagagaatt ttacttctaa tatagatagt gctattaata agatagtaaa
     2581 gttggctgac gataaagaag ttcaggctat tgtagtttca actgaccaag cagggctttt
     2641 acctgcttta caaaaggtaa aggaaaagcg tccagaaata ataactattt ctgcccctat
     2701 gggagatgat aaaaaccaat taagccaatt tgttgatgtg aatctagggg ttagtgcaga
     2761 ggaaagagga aaagtattag ctgaacgttc aaaagaaatg ggagcaaaag cttttattca
     2821 ttatgcctct acagatgatt tgaaagatgt taatatagcc aaaagattag aaatgattaa
     2881 agagacttgt aaaaatatag gattgccatt tgtacaagtt aatactccaa atataaatac
     2941 tgaagaagac aaaaacaaag ttaagcagtt tttaaatgaa gatattgaaa aacaagttaa
     3001 aaaatatgga aaagatataa atgtatttgg tgtaaatgag tatatggatg aagttatatt
     3061 gactaaggct ttagaattaa agtatatagt tgcagagcaa agcaatccat caccaataca
     3121 aacgtatcct agtgttatgg gtctaaaaat atctgaaaaa gatgctcaaa attatgataa
     3181 aataaacgac atgataagtg aaaaggctaa ggcttttggt atgtcaaata gacttggtgg
     3241 atatcctatg ccaatggatg cttttctacc atcagtagca atttatcttg ctactgaaat
     3301 ggtgaaacaa gatttaacac aagaagatgt atgtgaccca gattatttag aggcgtttac
     3361 agaattaaga tttggaatcg gttcagaatt tactccttta acagaggtac tttataatta
     3421 tcaatcagta attttaagcc aattaattta ttaatattaa ggaagtgtct caaaatgaac
     3481 tttttagttt atgaggcact atttttctgt taaatcaaat atatttttat ctttttaaca
     3541 attaaaaaga agcttatccc tatg
//
LOCUS       DAMJWP010000281         3538 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.288, whole genome shotgun sequence.
ACCESSION   DAMJWP010000281 DAMJWP010000000
VERSION     DAMJWP010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.288"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            248..625
                     /locus_tag="PC700_003383"
     CDS             248..625
                     /locus_tag="PC700_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2582649.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDATVTSKDVIEVMEQKYGWKQTTT
                     LTLLSRLVKRGFLNAQKIDRYTHYTVIVKHKDYLSFETKDFLSNIHDDSLKSLISALH
                     DDENIGKDTLDFLESYFDNLKNE"
     gene            618..1652
                     /locus_tag="PC700_003384"
     CDS             618..1652
                     /locus_tag="PC700_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HDF2582650.1"
                     /translation="MNNYMLYVFDNLIKTTIVCSFGICLLLFLKRYFFKRFSKRFNYY
                     IWLVIVFRMLLFLFNYTVMYEVKEPKENTVGNNITQIDVSIDNDFMLYLVYLWLFVTI
                     AIAVYTFMEYTKFKNLVVDVSYDIEDHDVNCIYKNLLKELDIKKKIELRGSDELISPA
                     GMGLFKSYIFLPDYTYSKDELTWILKHELMHFKNKDILIKFLVLSVKIMYWFNPLVYI
                     MSNKVNLDCELCCDESVLTDCSLKDKKAYALALIKSIKLSKNYNSGILTTEFNKTNLE
                     KRLDSIIKKKGKSGILMLLILFTLFSVTYFNFESISFDNSNYLRNIGILEKTGADLKE
                     EDDISMRVVN"
     gene            2131..2304
                     /locus_tag="PC700_003385"
     CDS             2131..2304
                     /locus_tag="PC700_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HDF2582651.1"
                     /translation="MHLVQLLCQQINGSDIDPDSKFGPATYNQVKILQGRLGVTKDGV
                     VGKATWQAVGERY"
     gene            complement(2389..3411)
                     /locus_tag="PC700_003386"
     CDS             complement(2389..3411)
                     /locus_tag="PC700_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDF2582652.1"
                     /translation="MNYNDLLKSARENFNGTCKVCKICNGLACAGDVPGMGGKGSGSS
                     FIENRKSLEKIKINMRVIHNVSKPDTSIELFGRKMSSPIFAAPVSGTLLNMGGKVSEK
                     EYIEPVVRGCSNSGIYAMVGDTNVDTFLLDNLDVLKDNSGNGIVFIKPWNNSKIIEKI
                     RLSEEAGAFAVGVDLDACGLINNQFQENPFSPKTIDEIRELVESTKLPFIIKGIMTVD
                     DALMAVESGASAIIVSNHGGRVLDYTPGTCEVLPDIAKSVKGKITILVDGGVRTGVDV
                     VKMLGLGADAVLMGRPFVTASFGGGLDGVEFFIEKVRNELCETMILTGCQNVKDIDGR
                     VIWNLL"
BASE COUNT         1367 a          467 c          497 g         1207 t
ORIGIN      
        1 cttgtgtgaa aatataggaa tttttattaa ataattttaa tgtggaaata acattctgca
       61 ctattataat taaatatgta aaaagcaaaa tacttgaaca actatatttt aaaatctaac
      121 ttttagtatt ttaaaaaatt agaaaattaa taaaaagtac aaaaatccac attttttcat
      181 aaagcgtttg actaaaaaat tcacatattg tagacttaaa gtagcaagag ttttgaggga
      241 ggtgtaaatg atgataagaa aaataccaca agcagaacta aaagtaatga aatttatttg
      301 gaaagtagat gctacagtga catcaaaaga tgttattgaa gttatggaac aaaaatatgg
      361 ttggaaacaa accactacac taacactttt atcgaggtta gtaaaaagag gattcttaaa
      421 tgctcaaaaa atagacagat atacacatta cacagtaata gtaaaacaca aagattattt
      481 aagttttgag acaaaggatt tcctaagtaa tatacatgat gattctttga aaagcttaat
      541 ttcagcatta catgatgatg aaaatatagg taaagacacg ctagattttt tagaaagtta
      601 ttttgataat ttaaagaatg aataattata tgctatatgt ttttgacaat cttataaaga
      661 caactattgt atgtagtttt ggtatttgtc tattattatt tttgaaaaga tattttttta
      721 agaggtttag taagagattt aattattata tttggttggt aatagtcttt agaatgttac
      781 tttttctatt taattataca gtaatgtatg aagtaaaaga gcccaaggaa aatacagtag
      841 gaaataatat tacacaaatt gatgtttcta tagataatga ttttatgctg tatttagttt
      901 atttatggtt atttgtaact atagccattg cagtttatac atttatggaa tacacaaaat
      961 ttaaaaatct cgttgttgat gtatcttatg atattgaaga tcatgatgta aattgtatct
     1021 ataaaaattt gttaaaagaa ttagacataa aaaagaaaat tgagttaaga ggttctgatg
     1081 aattgataag tccagcaggt atgggattat ttaaatctta catattctta ccagactata
     1141 cttatagtaa agatgagtta acttggattt tgaaacatga attaatgcat tttaaaaaca
     1201 aagatatttt aataaagttt ttagttttgt ctgttaaaat aatgtattgg tttaatccac
     1261 ttgtttatat aatgagcaat aaagtaaatc tagattgtga attatgttgt gatgaaagtg
     1321 ttttgactga ttgctcttta aaagataaga aagcatatgc gttagcactt ataaagtcaa
     1381 ttaaacttag taagaattat aatagtggta ttttaacaac tgaatttaat aagacaaatt
     1441 tggaaaagag acttgatagt attattaaga aaaaagggaa aagtggaata ttgatgttat
     1501 tgattttgtt tacattattt tcagttacat attttaattt tgaaagtatt agttttgata
     1561 attcaaatta tttaagaaat atagggattt tagaaaaaac aggcgcagat cttaaggaag
     1621 aagatgatat tagcatgaga gttgtaaatt aatagatttt tatagtatta taatttatta
     1681 acaaattagg agaagttttg aaattaaaaa tctaaaaaaa atcttagtag tcacagggag
     1741 ggtgactcta atatctatta gtttagtatt tgcaaatact aataatgatg taaatgtatg
     1801 aaaaagtaat gaagagcaaa caacaaattc atttacaata actggttacg aatttattta
     1861 tgaaaatgta ccacaaccta taaaatctga ttatgaaaaa aatgtaaaga attaaatata
     1921 aatccatcta aagatgatac aatctttatt aatgaaaaat ttatagattc atataatatt
     1981 aatatagatg attataatat tgaaccaaaa gcatttatta ttagatataa aaaagattta
     2041 aatcgttttg atgctactgg tcctataagt tatagcgtaa atataaatac cactgtagta
     2101 ggatatggac atgtagaaaa agggaatcct gtgcatttag tacaactttt atgtcaacaa
     2161 ataaatggtt ctgatattga tccagatagt aaatttggac ctgctacata taatcaagta
     2221 aaaatacttc aaggaagact tggtgtaaca aaagatggtg ttgttggaaa agccacttgg
     2281 caagctgtcg gagagcgtta ttagaaaaga ggttgtcgca ctaagaaata gggcgacaac
     2341 ttttttatat agaatatatt aatcagtatc ttagagtcta gaaatatttt acaataaatt
     2401 ccatataact ctaccatcta tatccttaac attttgacaa ccagtcaaaa tcatagtctc
     2461 acataactca ttcctaacct tctctataaa aaactccaca ccatcaagac caccgccaaa
     2521 agaagcagta acaaaaggtc ttcccatcaa aacagcatca gcacccaatc caagcatctt
     2581 aactacatca acaccagtcc taactccacc atcaacaagt attgttatct ttccctttac
     2641 agacttagct atatctggaa gtacctcaca agtaccagga gtataatcaa gtactcttcc
     2701 accatgattt gaaacaataa tggcactagc accagactct acagccatca aagcatcatc
     2761 tacagtcata attcctttta taataaaagg taatttagta gattcaacca attctctaat
     2821 ctcatcaatt gtcttaggag aaaaaggatt ttcttgaaat tgattattaa taagtccaca
     2881 agcatcaagg tctacaccaa ctgcaaaagc acctgcttct tcagacaatc taattttttc
     2941 tattatcttt gaattattcc aaggctttat aaatacaatt ccatttccac tattatcttt
     3001 caacacatct aaattatcca gtaaaaaagt atctacatta gtatcgccaa ccatagcata
     3061 aattccagaa ttagaacaac ctctaacaac aggctctatg tattcttttt cggaaacttt
     3121 accacccatg ttcaatagag tacctgatac aggtgcagcg aatataggag aactcatttt
     3181 tctaccaaat aattctatgg atgtatctgg ctttgaaaca ttatgtataa ctctcatatt
     3241 tatctttatt ttttctagac tttttctatt ttcaatgaaa gacgaaccac ttcctttacc
     3301 acccatacca ggaacatctc cagcacaagc taatccatta caaattttac atactttaca
     3361 tgttccatta aagttctctc tagctgattt tagtaagtca ttgtaattca tacaaaatct
     3421 ctccttttta taatattaaa attgagtatt actatattga aaatatctta attgtaaata
     3481 tagggatgtt attatgatga gctaaactag ataatacata ttagatattt tttattgt
//
LOCUS       DAMJWP010000282         3527 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.289, whole genome shotgun sequence.
ACCESSION   DAMJWP010000282 DAMJWP010000000
VERSION     DAMJWP010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.289"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(<1..396)
                     /locus_tag="PC700_003387"
     CDS             complement(<1..396)
                     /locus_tag="PC700_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HDF2582653.1"
                     /translation="MESKGTLSKNKVYILANGLFLVIALLNINLYTVSINMYRIILLW
                     IFGYSIVYISSFKTDEFINILKVGCLVLVFVSILGVVNLKIQKFQIVLLNLSIINMST
                     FIYLTISKVKKLRISPILIFYSAFVILDYF"
     gene            complement(544..1335)
                     /locus_tag="PC700_003388"
     CDS             complement(544..1335)
                     /locus_tag="PC700_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2582654.1"
                     /translation="MKLKKLLSVALVSAIAISAVGCSNKEDKKILVGASSNPHAKILE
                     VAKPLLKEKGYDLEVKIFDDYVLPNTALDEGSLDANFFQHIPFLEETVKEKGYKLTYT
                     SKVHIEPMGFYSEKVKSLDELKDGAVIAVPNDATNGARALKLLAKNKLIEVKDGELIT
                     KKDITKNPKNIVIKEMNAEQLPTVLKDVDGAVINSNYALTANLNPTKDAIVIESSDSP
                     YVNIIACRENNKDSDKIKALSEAMNSKEVKKFIQDEYKGSIVPAF"
     gene            complement(1371..2027)
                     /locus_tag="PC700_003389"
     CDS             complement(1371..2027)
                     /locus_tag="PC700_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2582655.1"
                     /translation="MNSLIDFLTTLFPNALLQTLYMVIVPTIVATILGFILAIILVVT
                     KPDGLKPNSTINSALGFIVNIFRSFPFMILIVAMIPITRLIVGTSIGETAAIVPITIG
                     AAPFIARIIESSLNEVDKGLIEAAKSFGATKRQIVFKVMIKEAMPSIVSGITLSIISI
                     LGYTAMAGAVGAGGLGNIALIYGYQRFDTAVMVYTVIALIILVQIIQGVGNLAYKKLK
                     "
     gene            complement(2020..2985)
                     /locus_tag="PC700_003390"
     CDS             complement(2020..2985)
                     /locus_tag="PC700_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HDF2582656.1"
                     /translation="MISIKNVNKYYGKIQVLKDVSIEIESGEIFGIIGHSGAGKSTLL
                     RCINGLEEYQEGSVLVSDKEVKSLNEKQMRDLRKELGMIFQHFSLLERKTVFDNVALP
                     LECFGYSKAEIKKRVLELLEVVGISEKKNDKPRNLSGGQKQRVAIARALALNPQVLLC
                     DEATSALDPNTTKSILSLLEDINKKLGITIIVVTHQMEVIKQICGRVAIMENGEVLEV
                     GDTEEIFLRNTKGLRKLIGEESIILPKGTNIKILFPKDISNEAIITTMARELNIDVSI
                     IFGKLEQFKDDILGSLIINISDKSGEHVKQYLTSKGIRWEEMINE"
     regulatory      complement(3091..3197)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
BASE COUNT         1112 a          614 c          359 g         1442 t
ORIGIN      
        1 aaagtaatct aatatcacaa aagcactata aaaaattagt attgggctta ttcttagttt
       61 ttttacttta gaaatggtta agtatataaa agtagacata ttaattatgc ttaagttaag
      121 caatacaatt tggaattttt gaatttttaa atttactact cctaaaatac ttacaaaaac
      181 taatacaaga caccctactt tcaatatatt tataaattca tctgttttaa agctacttat
      241 ataaactata gaatatccaa atatccaaag taaaattatt ctatacatat ttatagaaac
      301 tgtatacaaa tttatattta aaagtgcaat aaccaaaaat aacccattag ctagtatata
      361 gaccttattc ttacttaaag ttcctttaga ttccatattc cccaccactt cttcattttt
      421 atatagtttt acctataaat atgttaccac aattcttcca aaaaatttgt cactttctag
      481 ttataaaaaa aaatgagtaa atatttttaa ttttactcat tttgtagatt ttgtatattc
      541 agtttaaaaa gctggaacta tacttccttt atattcatct tgtataaatt ttttaacttc
      601 tttactattc atagcctctg ataaagcctt aatcttatca ctatctttat tattttctct
      661 acaagctatt atatttacat atggagaatc acttgattct attactattg catccttagt
      721 aggatttaag ttagctgtta aagcataatt agaatttatt actgctccgt caacatcttt
      781 taatactgtt ggtaattgct ctgcattcat ttcttttatt actatatttt taggattttt
      841 agttatatct tttttagtta taagctctcc atcttttact tctatcaatt tattttttgc
      901 taataattta agtgctctag ctccattagt tgcatcattt ggcacagcta taacagctcc
      961 atcttttaat tcatctaatg acttaacttt ttctgaatag aatcccattg gctctatatg
     1021 aactttagat gtataagtaa gtttatatcc tttctccttc acagtttcct ctaaaaatgg
     1081 aatatgttga aagaagttag catccaaaga cccttcatct aaagctgtat ttggaagtac
     1141 ataatcatca aatattttta cttctaaatc atatcctttt tcttttaaca aaggttttgc
     1201 aacttccaat atctttgcat gtggatttga tgaagctcct actagtattt ttttatcctc
     1261 tttgtttgaa caacctactg ctgatattgc tattgctgac accaatgcta ctgataataa
     1321 tttctttaat ttcattttta ctccccctct ttaaaatgtt tataattaat ttattttagt
     1381 ttcttataag ccagatttcc tactccctgg attatctgaa ctaatattat aagtgctatt
     1441 acagtatata ccattactgc tgtatcaaat ctttgatatc cataaataag tgctatatta
     1501 cctaatcctc cagctcctac tgcacctgcc atagctgtat aaccaagtat agatattatt
     1561 gataatgtaa ttccagatac tattgaaggc atagcttcct ttatcataac tttaaatact
     1621 atttgtcttt ttgtagctcc aaaagatttt gctgcttcta tcaatccttt atcaacttca
     1681 tttaatgagc tttctataat tctagcgata aacggtgctg ccccaatagt tattggcact
     1741 attgcagcag tttcccctat acttgttcca acgattaatc tagttattgg aatcattgca
     1801 actataagta tcataaatgg gaaacttcta aatatattta ctataaatcc taatgcacta
     1861 tttattgtgc tatttggttt taatccatct ggttttgtaa ctactaatat gattgctaat
     1921 ataaatccta atattgttgc cactatagtt ggtactataa ccatatacaa agtttgtaac
     1981 aatgcatttg gaaatagtgt tgttaaaaag tcaattaaac tattcattta tcatttcctc
     2041 ccatcttatg cccttactag ttagatattg ttttacgtgc tctccacttt tatctgaaat
     2101 gtttataata agtgaaccta aaatatcatc tttaaattgt tctaatttcc caaatattat
     2161 agacacatct atatttagtt ctcttgccat agtcgtaata atagcttcat tagaaatatc
     2221 tttagggaat agtatcttta tatttgttcc ttttggcaat attatacttt cttcacctat
     2281 taattttcta agtcctttag tatttcttaa gaatatttct tctgtatctc ctacctctaa
     2341 tacttctcca ttttccatta ttgctactct tccacaaatc tgctttataa cttccatttg
     2401 atgagttaca actattattg ttattcccaa ttttttattt atatcttcaa gcaatgaaag
     2461 aattgacttt gtagtatttg ggtctaatgc tgaagttgct tcatcacaaa gaagtacttg
     2521 tgggtttagt gctaaagctc tagcaattgc aaccctttgt ttttgtccgc cacttagatt
     2581 tctgggttta tcatttttct tttcagatat tccaactact tctaataact ctaaaactct
     2641 ttttttaatt tctgcctttg agtatccaaa acattctaat ggcaatgcta cattatcaaa
     2701 tacagttttt ctttcaagca atgaaaagtg ttgaaaaatc attcctaact cttttcttaa
     2761 atctctcatt tgcttttcat tcaatgactt tacttcttta tcagaaacaa gtacactgcc
     2821 ttcttggtat tcttctaatc cgtttataca tctcagtagt gttgatttcc ctgcaccact
     2881 atgacctatt attccaaaaa tttctccaga ttctatttca atacttacat ctttaagcac
     2941 ttgaatcttt ccataatact tattaacatt ttttatgcta atcataaaat ttcctcccat
     3001 atattctgct cagtttttat gtttatcctc tcttatgtat tacaacaaaa taaaagcccg
     3061 agtataaact caggcttaga aagctattta tactcatcta tcaactatat tagttgcagg
     3121 atttagcacc aatgtatata aaatatactg gttgccgggt ttcaaagggc cagtccctcc
     3181 accactcttg ataagacgtt atttaattca attctattat catttgttgc tatgttatat
     3241 atgatatatt cattaatagt atttgtcaat aaatttattt aaatttttta ttttttatta
     3301 tcgttaatta actattttta attagttttc tagctcctaa cactttatat tttaatgtct
     3361 atataactat agaacatcat tataagaaat aaattaaata acatcaatct atctaaatca
     3421 tctgtaaagt aaattaagaa aattaacatt ttatccaaac tctatcctac tattaaataa
     3481 aataatttaa atacaatgat gattatatag ctctaatact ttagact
//
LOCUS       DAMJWP010000283         3447 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.290, whole genome shotgun sequence.
ACCESSION   DAMJWP010000283 DAMJWP010000000
VERSION     DAMJWP010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.290"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            1..1074
                     /locus_tag="PC700_003391"
     CDS             1..1074
                     /locus_tag="PC700_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDF2582657.1"
                     /translation="MNKFKLKRSILISLIILIFIFIVYFVSIELKDGSLVNNQYKGKI
                     KSANLSVDYEINQVMKDIDKLGLNTVNVPIIINVESINSDIMSIDNNSKEKAIKLIKK
                     LNKKGISTILEAYPWVENGKLYETDWNPINKKRFFYTWQNAILNELIIDVANPLDVNV
                     LNIGSNFVHLEEYQQNWGEIIDFVQSKFDGLVTYRTNWWYTKKDDSSSKLFYEQKLNN
                     NFFDKLDFISIAAYFELSNKPVNTVDELISALHSSTVNNRGQNIKQEIYNLYKAHRKP
                     IFFGELGFSNRESASSQPWNHTPSKAVNGEEQARCFEAYKKVFENEDWINGFSVFCVG
                     KIDDEKNFYPSKESIKVIKSWYE"
     gene            1128..2252
                     /gene="wecB"
                     /locus_tag="PC700_003392"
     CDS             1128..2252
                     /gene="wecB"
                     /locus_tag="PC700_003392"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HDF2582658.1"
                     /translation="MNDIKVMTVFGTRPEAIKVAPLIKELEKRENIKSIVCVTAQHRE
                     MLDQVIETFNINVDYDLDIMEKGQSLNDITCKILNKLPLILNKENPNIILVHGDTTTT
                     LATSLTAFYNKALVGHIEAGLRTYDKYSPFPEELNRQLTGIIADMHFAPTNLARKNLI
                     SEGKPNNNIFVTGNTAIDALKMTIKENYNHPIIDEIGNDRMILLTSHRRENLGKPMKN
                     IFRAIKRIVDDFEDVQIVYPIHLNPKIRTIADEIFGKFPEKIHIIEPLDVADFHNFLN
                     KSYMIMTDSGGIQEEAPSLGKPVLVLRDKTERTEGIEAKTLKLVGTNEDRIYNSVSDL
                     LINKDSYVQMSKASNPYGDGNASKYIVDIIIKKFNCKYLN"
     gene            2330..3304
                     /locus_tag="PC700_003393"
     CDS             2330..3304
                     /locus_tag="PC700_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDF2582659.1"
                     /translation="MARKLIKNLGKSKSVKRVKLLFKKIFITVFIVASIVAIFNITKY
                     FEELYKVRDLKSTKIEYYMDVADEAGDGKVQLSWKALLAIDMVIHDEDLSNIKKKDTL
                     DIGEKFIVEDKNDKGEKVYKVKKFNKVLSELKFDSSQKSRARKYMKDLEYTYLGNKQL
                     DSSDEKIKFIKKLEDSAIREYIDYGILPSVTIGQAILESGWGNSKLTKQSNNLFGIKA
                     DKAWKGKSVEISTSEHYNEKIVASFRSYNSLQDSVKDHSLFLINNKRYRKHGLFEAKD
                     YISQAQALENAGYSTAEDKKGNRIYAELLIDIIRSYNLQLIDNKVETK"
BASE COUNT         1440 a          337 c          604 g         1066 t
ORIGIN      
        1 atgaataaat ttaaattaaa aagaagcatt ttaatctcat taataatctt aatttttata
       61 tttatagtgt actttgtaag tattgagtta aaagatggga gtctagtaaa taatcaatat
      121 aaaggtaaga ttaaatcggc caatttatct gtagattatg aaataaatca agttatgaag
      181 gatatagata aattgggatt gaatacagta aatgtaccta taattataaa tgtagagtcc
      241 attaattctg atattatgtc aatagataat aatagtaaag aaaaggcaat taagttaata
      301 aaaaaattaa ataaaaaagg tatttcaaca atattagagg catatccatg ggttgaaaat
      361 ggaaaattgt atgagactga ctggaaccct ataaataaaa aaagattttt ttatacttgg
      421 caaaatgcca tattaaatga acttataatt gatgtagcaa atccactgga tgtaaatgtt
      481 ttaaatattg gttctaattt tgtacatttg gaagaatatc aacaaaattg gggtgaaata
      541 atagattttg tacaatccaa atttgatggt cttgtaacgt acagaactaa ttggtggtat
      601 acaaaaaaag atgattcaag tagtaagttg ttttatgaac aaaaattgaa caataatttt
      661 tttgataagt tagattttat atctatagca gcatattttg agttaagtaa taaacctgtt
      721 aatacagtgg atgaattaat ttctgcctta cactcatcta ctgtaaacaa tagaggacaa
      781 aatataaaac aagaaatata taacctttat aaagctcata gaaaacctat attttttgga
      841 gaattaggtt tttcaaatag agaaagtgca agttctcagc cgtggaatca tactccttca
      901 aaagctgtaa atggcgaaga acaagcaagg tgttttgagg catataagaa agtttttgaa
      961 aatgaagact ggataaatgg tttttctgtg ttttgtgtag gtaaaataga tgacgaaaaa
     1021 aatttttacc catcaaaaga gagtataaaa gttattaagt cttggtatga ataattttaa
     1081 aagtgtagaa tttatataga ttaaatagat gattaggaga gatataaatg aatgacatca
     1141 aggttatgac tgtatttgga actagacctg aagctatcaa agtagcacct ttaataaagg
     1201 aacttgaaaa aagagagaat ataaaaagta ttgtttgcgt tacagctcaa catagagaaa
     1261 tgcttgacca agttatagaa accttcaata taaatgtaga ctatgatttg gatataatgg
     1321 aaaaaggaca aagcctgaat gatataacat gtaagatttt aaataaactt cccttgattt
     1381 taaacaagga aaatccaaat ataatattgg tacatggaga tacaaccaca actctagcta
     1441 caagtttgac tgcattttat aataaagctc ttgttggtca tatagaagct gggcttagaa
     1501 cctatgataa atattctcct tttccagagg agttaaatag acaacttaca ggtattattg
     1561 ctgatatgca ctttgctcct actaatttgg ctagaaaaaa cttgatttct gaaggaaaac
     1621 ctaataataa tatatttgta actggtaata ctgctattga tgctttaaag atgacaataa
     1681 aagaaaacta taatcatcct ataattgatg agattggtaa cgataggatg attttgttaa
     1741 cctctcacag aagagaaaat ttgggtaaac ctatgaaaaa tatattcaga gctataaagc
     1801 gaatagtaga tgattttgaa gatgtacaga tagtatatcc catacattta aatcctaaaa
     1861 tacgaactat agctgatgag atatttggaa aatttcctga aaaaattcat attatagagc
     1921 ctttagatgt agctgatttt cataactttt taaataaatc ttatatgata atgactgata
     1981 gtggaggaat acaggaagaa gctccatcat taggtaagcc agtccttgtg ttgcgtgata
     2041 aaacagaaag aacagaagga attgaagcaa agacacttaa attagtaggt actaatgaag
     2101 atagaatata taattcagtg agcgatttat taataaataa agatagctat gttcagatga
     2161 gtaaagcatc taatccatat ggagatggaa atgcaagcaa atatatagtg gatataataa
     2221 ttaaaaaatt taattgcaaa tatttaaatt agataaggtc taaaacatta actaattgat
     2281 ataagattta tgataatatt aaggaataca atttttaagg gggagtaata tggctagaaa
     2341 attaataaaa aatttaggta aaagtaaaag tgtaaagaga gtgaaacttt tatttaagaa
     2401 gatttttatt actgtattca tagtagcaag tatagttgct atttttaata taacgaaata
     2461 ttttgaggaa ttatacaaag taagggattt aaaaagtact aaaattgaat actatatgga
     2521 tgtggctgat gaagcaggag atggaaaggt ccaattaagt tggaaggcct tacttgctat
     2581 agacatggta atacatgatg aagatttaag taatataaaa aagaaagaca cattggatat
     2641 aggggaaaag tttatagtag aagataaaaa tgataaaggc gaaaaggtgt ataaggtaaa
     2701 aaagtttaat aaggtattaa gcgaattgaa atttgactct tctcaaaaaa gtagagcaag
     2761 aaaatacatg aaagatttgg aatacacata ccttggaaat aaacaattag atagtagtga
     2821 tgaaaaaatt aaatttataa agaagttaga agactcagct ataagagaat atattgatta
     2881 tggaatattg ccctctgtaa caattggaca agctatatta gaatctggct ggggaaattc
     2941 taaacttaca aaacagagta ataatttatt tggtataaaa gcagataaag catggaaagg
     3001 aaaaagtgta gaaatttcaa cttcagagca ttataatgaa aaaattgtag ctagttttag
     3061 gtcgtataat tcattacaag attctgtcaa agaccacagt ttatttttaa ttaataataa
     3121 aagatataga aaacatgggt tgtttgaggc aaaagattat attagtcaag ctcaagcatt
     3181 agaaaatgca ggatatagta cagcagaaga taaaaaagga aatcgtatat atgcagagct
     3241 attaatagat attattagga gttataattt gcaattgata gacaataaag ttgagacaaa
     3301 ataattgtta actttatatg aggagataac aagataagat tcttttctac aaaaagaata
     3361 gaaaaattgt aaaaaaaact aaaaaaatgt attgacttgt tatttaaaag gtgatattat
     3421 tattactgta ctcgaaagag aacaaaa
//
LOCUS       DAMJWP010000284         3446 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.291, whole genome shotgun sequence.
ACCESSION   DAMJWP010000284 DAMJWP010000000
VERSION     DAMJWP010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.291"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..1111
                     /locus_tag="PC700_003394"
     CDS             <1..1111
                     /locus_tag="PC700_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HDF2582660.1"
                     /translation="NISIIISDESMDVIDLYDDNNNEVAKKVNFSIFNPKFNLYMYQY
                     KNLINYLDKSICMGALSSGSTSVPKVLYRTYESWAGFFPIQNGIFNISGESILFVNGT
                     LSFTGNLNSIMSVLYEGGSIVISSGLNCKSWIRTIKQYNVTNIYLIPTKLQLLVKHLK
                     EPVLKVVSIFTGSQLLFEYTARNLKKYMPKSEIILYYGASELNYITYLCYDELIEKPL
                     SVGRPFPGIDIYIRGGKIFVNTEYAVYNATKPYSVNDIGYYDNDGYLIFEGRSDDIVN
                     IGGFKVSSAKIENEVKKIPQIEDAIVLPYSDTIRGNQIVLFVITIDKITKKDLLIKMK
                     DNLMKNEIPKKIIFLNSFPYTSSEKIDRLALLKML"
     gene            1187..2062
                     /gene="dpsA"
                     /locus_tag="PC700_003395"
     CDS             1187..2062
                     /gene="dpsA"
                     /locus_tag="PC700_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HDF2582661.1"
                     /translation="MSNQYDITVIGGDLRQVYMVKKLINKGYSVVTYGISNEIINGIV
                     GKSTSLAEALSKSSIILCPIPFTKNQVNIENTDDCLDATIDNLLINLSPNQILFGGNI
                     PTKVCKFCEQHSIQIYDFMKMNDVAILNAVATAEGAIAEAIKRSIINIHQSNCLVLGY
                     GRCAQILAKKLYALDANVCVGARNNDARSTANAFGYSSISLSQLDEKLLQFDYIFNTI
                     PECILTKERLEKVSQEVTIIDIASSPGGVDYSVVKEVGINATLCLGLPGKYSPKTSGE
                     ILVNAIENIINERRD"
     gene            2065..2655
                     /locus_tag="PC700_003396"
     CDS             2065..2655
                     /locus_tag="PC700_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HDF2582662.1"
                     /translation="MRLEGLTIGVGFTGSFCTYDKIFIELENLVKEGANVHTIFSDVS
                     QNIDCRFGNSEEFMKKAYELTGNKPIVTIEEAEPFGPKGIADIIIIAPCTGNTAAKLA
                     NGITDSPVLMAAKGHLRNDKPLVISISTNDALSFNFKNIGILLNSKNIYFVPFGQDNC
                     KAKPNSMIAHTELIIPTIELALENKQLQPVIKSPHQ"
     gene            complement(2863..3063)
                     /locus_tag="PC700_003397"
     CDS             complement(2863..3063)
                     /locus_tag="PC700_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582663.1"
                     /translation="MKKLKTISIFSLIISVILTIGGIGIVTYYVDNLFIRGLSVFVLI
                     MSSSFVSTTVRLIFEESKRYKF"
BASE COUNT         1321 a          410 c          513 g         1202 t
ORIGIN      
        1 taatatttca atcattatat ctgatgaatc tatggacgtt attgatttat atgatgataa
       61 caataatgaa gtagcaaaaa aagttaattt ttctattttt aatcctaaat ttaatctata
      121 tatgtatcaa tataagaacc ttataaatta tttagataaa agtatttgta tgggagcttt
      181 atcttcaggt tctacaagtg ttccaaaggt attatataga acatatgaga gttgggctgg
      241 attttttcct atacaaaatg gtatttttaa tatctctggg gaatcaatct tgtttgttaa
      301 tggaacactt agttttacag ggaatctaaa ttctataatg tcagtattat atgaaggagg
      361 tagtattgta ataagttcag gtcttaattg taaatcatgg ataaggacaa taaaacaata
      421 taatgtaact aacatatacc ttatacctac aaaacttcaa cttttggtta aacacttaaa
      481 agaaccagta ctcaaagtag taagtatttt tacagggtct caattattat ttgaatatac
      541 tgcaagaaat ctaaaaaaat atatgccaaa gtctgaaatt attttatact atggggcaag
      601 tgaattaaat tatattacat atttatgcta tgatgaacta attgaaaaac ctctaagtgt
      661 aggcagacca tttccaggaa tagatatata tattagagga ggaaaaattt ttgtgaatac
      721 agaatatgca gtatataatg caaccaaacc ttattctgtc aatgatattg gatattatga
      781 taatgatgga tacttaatat ttgaaggaag aagtgatgac attgtaaata ttggaggatt
      841 taaagttagt tctgctaaga ttgaaaatga agttaaaaaa attcctcaaa ttgaagatgc
      901 aattgtattg ccgtactcag acactataag aggaaatcaa attgtattat ttgtaataac
      961 tatagacaaa attacgaaga aagatttgtt gattaaaatg aaagataatc taatgaaaaa
     1021 tgaaatccca aagaaaatta tctttttaaa ttcatttcca tatacctctt ctgaaaagat
     1081 agatagatta gcattattaa aaatgttata agtataatat ttagtaattt tagtgaacca
     1141 atgtattgat aaaaacatat tgtatatagt aaaggaggct tacaatatgt caaatcaata
     1201 cgatataact gttattgggg gagacctgcg acaagtgtac atggtaaaaa aactaattaa
     1261 taaagggtat tctgtagtca cttatgggat atcaaatgaa attataaatg gaatcgttgg
     1321 aaaatcaact tcactagctg aagcactatc caaaagttcc attattttat gtccaattcc
     1381 atttacaaag aatcaagtta atattgaaaa tacggatgat tgtcttgatg ctactattga
     1441 taacttgcta attaatttat ctcctaatca aatattattt ggaggtaaca taccaactaa
     1501 agtttgtaaa ttttgcgagc aacactctat tcagatttat gattttatga aaatgaatga
     1561 tgttgctatt ttaaatgcag ttgctacagc tgaaggggct atagcagaag ctattaaaag
     1621 aagtataatc aatatacatc aaagtaactg tttagtatta ggttatggaa gatgtgcaca
     1681 aatacttgca aaaaaattat acgctttgga tgcaaatgta tgcgttggtg caagaaataa
     1741 tgatgcaaga agtacggcta atgcatttgg atattccagt atttctttaa gtcaacttga
     1801 tgaaaaactt ttacaatttg attatatttt taacaccata ccagagtgta ttttaacgaa
     1861 agaaagatta gaaaaagtat cacaagaagt aactattata gatatagctt cttctcctgg
     1921 aggagtagat tattctgttg ttaaagaagt agggattaat gcgactcttt gccttgggct
     1981 tcctggtaaa tattcaccaa agacatctgg tgagattctt gttaacgcta tagaaaatat
     2041 tataaatgaa agaagggatt agaaatgaga cttgaaggac ttacaattgg agtaggattt
     2101 actggttcgt tttgtactta tgataaaata tttatagagt tagagaattt ggttaaagaa
     2161 ggagctaatg tacacactat attttcagat gtatcacaaa atatcgattg tagatttgga
     2221 aattctgaag agtttatgaa aaaagcatat gaacttacag gaaataaacc tatagttacg
     2281 attgaagagg cagaaccatt tggacctaaa gggattgctg atattataat aatagctcct
     2341 tgtactggaa atactgctgc aaaattagca aatggtatta cagattcacc agtattaatg
     2401 gcagcaaaag gtcatcttcg taatgataaa ccactagtaa tatcaatatc aacgaatgat
     2461 gctctaagct ttaatttcaa aaatattggt attctcctta attctaaaaa tatatatttt
     2521 gtaccttttg gtcaagataa ttgcaaagca aagcctaatt ctatgattgc acatactgag
     2581 ctaataattc caactataga acttgctcta gagaacaaac agctacagcc tgttataaaa
     2641 agtccacatc aatagaattg gttcttataa tattatgtat tagatacaat actagcaaag
     2701 aggctgtctc aaaatagaga gaagcctctt tttcattgtt gaaatgatga aaatatattt
     2761 aatttcatac aaaaaaatgc atcatgaact aaaaagtcta ttttgagcta atctccgtaa
     2821 tttttggtat attaaaatta tttacttaat ccaattttta aattagaatt tatatcgttt
     2881 tgactcttca aatatcaatc taactgttgt tgatacaaaa gaagaggaca taattaaaac
     2941 aaatactgat agtcctctaa taaacaaatt atctacataa taagttacta ttccaatacc
     3001 tccaatcgtt aaaattacac ttataatcaa agaaaaaatg ctaattgttt ttaatttttt
     3061 catgcttatc ctccttctta taaacttcaa atttatctat ataatattaa atatatttta
     3121 tttcatagta tataaaaatc atgaatatat atttttttaa ttatactata tgttgtaaat
     3181 gtttgtacat ttaactatat ttactttaac ttgttaatca agttattatt ttcattttca
     3241 agtattttta tttttaaact tgatagaata tcatattaaa aattcttttt ataaagtttt
     3301 atttgcaaaa aatacaaaaa tataaacgtt aaaaaaataa cgttatcctt aagtttttag
     3361 atgattttat tagtgatttg cgtaaaaaat aaagtgatat ctagttaaat taagtatttg
     3421 taaagctaga tattattagt agaaat
//
LOCUS       DAMJWP010000285         3425 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.292, whole genome shotgun sequence.
ACCESSION   DAMJWP010000285 DAMJWP010000000
VERSION     DAMJWP010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.292"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            149..1747
                     /locus_tag="PC700_003398"
     CDS             149..1747
                     /locus_tag="PC700_003398"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HDF2582664.1"
                     /translation="MSLLVFGHKNPDTDSICSAISLAHLKNKLGIKATAYALGDIRKE
                     AKYALDYFKVDAPEILDNVRIQVRDLNYDKVVPLTPTSSILEAYNLMDEKNVKTLPVT
                     YDDGTFAGIITMKEIAKNLLHQHFTTIHTSLSNICKNLNGTILVDCGEDIKGRVVTLS
                     FGMETLIETLKEGDIAIVGDRYDSIEYAIDTKVKLLILTNHTEISKDLLALAKINGVS
                     VVSVESDNYKTSNVINQCSFLDSIEATENLITFKEDDYMDEIKEIMLETNFRSYPILD
                     DDNKFLGLVSKGHLLNPSKKNVVLVDHNEYAQSADGIEQANIVEIIDHHKLGGISTDV
                     PMSFRVMPVGCNSTIIYQMYKENNVEIPYEIAGLLLSAILSDTLLFKSPTTTDMDKKA
                     CEELSKIAKVDMEKYAMDMFKCGTSLDEYTIEEIVNMDFKEFNMSGHRVGIGQVFTLD
                     IDSIFAKKDEFLSYINSTDYDKLILAVTDIIKEGSYLIYKAEDSLIANAFDVEGVQGT
                     FAPGVVSRKKQLVPNLTTAIKNFK"
     gene            complement(1982..2173)
                     /locus_tag="PC700_003399"
     CDS             complement(1982..2173)
                     /locus_tag="PC700_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium protein"
                     /protein_id="HDF2582665.1"
                     /translation="MAGLTTEPGGGGVLSGYFAGFLFGGTTFTINNFSSTTVGIRNGQ
                     SAGTAATLTIFRIADTVMT"
     gene            complement(2342..2935)
                     /locus_tag="PC700_003400"
     CDS             complement(2342..2935)
                     /locus_tag="PC700_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HDF2582666.1"
                     /translation="MRIPDILKDKSILKAELFIHIDSNKNHIFKEKVDIEIKRISEYY
                     NLRTITWNDRVSMENIRGYLPIGISDTSNYICLNITGTIKAWAMNKYPNYGLALSLNY
                     PYQIFEFTSSRDCNKPYILVTFEDRIIDNCYPKCECLPIRITGPMGPRGATGSIGPMG
                     ATGPTGATGNSSQPIANFLVNAPSPQTLNNGNAITGW"
     gene            complement(2936..3082)
                     /locus_tag="PC700_003401"
     CDS             complement(2936..3082)
                     /locus_tag="PC700_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium protein"
                     /protein_id="HDF2582667.1"
                     /translation="MRKIILYLNDDTFISKKYPDKNFSNLDYCLIGSKCSNSFVKEKL
                     ITFF"
BASE COUNT         1236 a          474 c          462 g         1253 t
ORIGIN      
        1 attaaatttg tagtatttat gtaagaaata aattttatat aatatttttt cactaaattt
       61 ttttcttttt acaacaaata ctcactacct tttgatataa tatttttatc aattattatt
      121 atattcaagt tttgaaagga gtattaaaat gagtttatta gttttcggac ataaaaatcc
      181 agatacagat tcaatatgtt ctgcaatttc attagcacat ttaaagaaca aactaggaat
      241 aaaagcaaca gcttatgctt taggagatat cagaaaagaa gctaaatatg ccttggatta
      301 ctttaaggta gatgcacctg aaattttaga taatgttaga atacaagtta gagatttaaa
      361 ttatgataaa gtagtacctt taacaccaac ttcatctata ttagaggctt ataaccttat
      421 ggatgaaaaa aatgttaaaa cactacctgt aacttatgat gatggaactt ttgctggtat
      481 cattactatg aaagaaatag ctaaaaatct acttcatcaa cactttacaa ctatacacac
      541 atcactatca aacatttgta aaaaccttaa tggtactatt cttgtggact gtggtgaaga
      601 tataaaaggt agagtagtca ctttatcatt tggaatggag actctaatag agactttaaa
      661 agaaggagac atagctattg ttggagatag atatgatagt attgaatatg ctattgatac
      721 aaaagttaaa cttttaatac ttactaatca tacagagatt tcaaaagatt tgctagctct
      781 tgctaagata aatggagtgt ctgttgtttc tgttgaaagt gacaattata aaacttctaa
      841 tgtaataaat caatgtagtt tcttagactc tatagaagct acagaaaatt taattacatt
      901 caaagaagat gattatatgg atgaaataaa agagattatg ctagaaacaa acttcagatc
      961 ttatccaata ttagatgatg ataataagtt tttaggttta gtttcaaaag gtcatctatt
     1021 aaacccatct aaaaagaatg ttgttttagt tgaccataac gaatatgctc aaagtgctga
     1081 tggaattgaa caagcaaaca tagttgaaat aattgaccat cataaacttg gtggtatttc
     1141 tactgatgtt cctatgtcat ttagagttat gcctgttgga tgcaatagta ctataattta
     1201 tcaaatgtac aaagaaaaca atgttgagat accttatgaa atagctgggc ttcttttatc
     1261 tgctatatta tcagatacat tattatttaa gtctccaaca acaactgata tggataagaa
     1321 agcttgtgaa gaattaagta aaatagcaaa agtagacatg gaaaaatacg ctatggatat
     1381 gtttaaatgc ggtacttctt tagatgaata tactatagaa gaaatagtaa atatggattt
     1441 taaagaattt aatatgagtg gacatagagt tggtattgga caagtattta ccttagacat
     1501 agactctata tttgctaaaa aagatgagtt tttatcttat ataaattcga ctgattatga
     1561 caaactaatt cttgcggtta cagatataat caaagaaggt tcttatttaa tctataaagc
     1621 agaagatagc ttaattgcta acgcatttga tgttgaaggt gttcaaggta catttgcacc
     1681 aggtgttgta tcaagaaaaa aacaattagt ccctaattta actactgcaa tcaaaaattt
     1741 taaataatat aaaattttta aataacgaaa actgtctcaa atctatattt gagacagttt
     1801 tttattatac atatattgat aataaagtta aataaaaatc cataaattta aaatttttat
     1861 gaatatattt tattattatt gttattttat tcattctaaa ataggtaaaa ctcaatatat
     1921 aaaaatactt ttgtcattta aataaaacct aaatagtttt taagattatt ttagacacgt
     1981 tttaagtcat aacagtatca gctattctaa atatcgtcaa agtagccgca gttcctgctg
     2041 attgcccatt tcgtatccct actgttgtag atgaaaaatt atttattgta aaagtagtcc
     2101 ccccaaataa aaaaccagca aaatatccag aaagtactcc tccgcctcct ggctcagtag
     2161 taagccctgc caaatctttt cctcctaaaa ttgggaatag gatagcaaaa gaatattgat
     2221 ttatacttga tgaacctggt tgtaatgcta ctgaaactga tatataatac acaccatttt
     2281 cttgtactgt aaacgtacca tttgcatcta ctgttatact tgaactattt cctattattg
     2341 tttaccaacc tgttatagca tttccattat ttagtgtttg tggagatggt gcatttacga
     2401 ggaagttagc aattggctga gaggaattgc ctgttgctcc tgttggacct gttgctccca
     2461 ttggtcctat acttcctgtc gctcctcttg gtcccattgg acctgtaatt ctaattggaa
     2521 gacactcaca tttaggataa caattatcta taattctatc ttcaaatgtt acaagtatat
     2581 acggtttgtt acaatcccta ctagatgtaa attcaaaaat ctgataaggg taatttaaag
     2641 ataaagctaa cccataatta ggatatttat tcattgccca tgcttttata gttcccgtaa
     2701 tatttaaaca aatatagttg gatgtatcac ttatcccaat tggtaaatat cccctgatat
     2761 tttccataga cactctatca ttccatgtta tagttcgtaa attataatat tcacttattc
     2821 ttttaatttc aatatctact ttttctttaa aaatatgatt cttatttgaa tcaatatgaa
     2881 taaataactc tgcttttaat atacttttgt cttttaatat atctggtatt ctcacttaaa
     2941 aaaaagtaat caacttttct tttacaaaac tatttgaaca tttacttcct attaagcaat
     3001 aatctaaatt actaaagttt ttatctggat attttttaga tataaaagta tcatcattta
     3061 aataaagtat aatttttctc atttataatc cctccatttt gggtctatat catactatgt
     3121 ttaaaataca tgcttagtta caatacaatt aaaatttata gtattaaatt tttatataat
     3181 aatgctgatt tctacaaaaa aagaacatgt cattagaaac ttctaatagc atgttctttt
     3241 tcattattat atattacact ttaaattatt ccttgactgg cttagctggt gtaaactaaa
     3301 ccagtctttc taaaatatta agcacttttt tcttattatg ttttttagga tttattgtct
     3361 ctattattgt aaaccaaagt tataaaattg taaatagaat atattaccat aacaatattt
     3421 tttta
//
LOCUS       DAMJWP010000286         3405 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.293, whole genome shotgun sequence.
ACCESSION   DAMJWP010000286 DAMJWP010000000
VERSION     DAMJWP010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.293"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(<1..2049)
                     /locus_tag="PC700_003402"
     CDS             complement(<1..2049)
                     /locus_tag="PC700_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HDF2582668.1"
                     /translation="MKKFIISIISLVLFFSNISLIYKVNADETKVKNYKFERDITIDG
                     VIGSNSTFFEVNKNWDIEEVLLHLNFSKSQILNGDVSSLTVLINNVPIKSIKLNAKTN
                     YKNTLEVLVPKDYIIQGYNEIKIKTYKTISDKICQDDSNTGNWMVIHKESYISIRYKQ
                     KKVENSINEYPYPYAEIENNHKLDTTIVVPDNMTRGETTAVFNLASSFGKITKNDDLK
                     LDVKLYSEMKNWSDDNIIYIGKPENTAEEILDILSIKEQTLLSSNCIIKQVDSPYNKN
                     KKMMVVIGSNEDDLIKASNLLIENRLSNQVLSSSVLVNKETNIKINREQKLNLGHLTL
                     KDLGYSDFLLEGAFNQQALFDVKIPTGKVLDDGSKIILNLRYSDNLDFEKSLVTVSIN
                     DVIVGSKKLDRSHSNNDKLELKIPKDIDNKNYYQVKLTFNLSIKNSNCVTRESNNPWA
                     YVSNNSYLALSTKENETLSFENYPYPFVRDDEFNDLTVIMPDYSGSQAMTWMFRLGVT
                     LGTNINSHNGNINVIRGKEFSDKYKDTNIVVFGVPHNNSVIKMLNNNLNIKFDKNYSN
                     FISNDKISFIDDYGKNISTIQLIKSPYNNQKNIMVISSMNEKNLYLGMDYLLNKSKVN
                     DLKGDTLIIDEYGEVEDLAYNLKPKKEVKDSSWNMSINKTTKVFLMISFITIIVVMIL
                     S"
     gene            complement(2072..3328)
                     /locus_tag="PC700_003403"
     CDS             complement(2072..3328)
                     /locus_tag="PC700_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003362983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2582669.1"
                     /translation="MRDLNVSEYLFVFSLFSIWSLLLINIILAMGGYIFYFKNFDKEI
                     KEIDEYPMISILVPAHNEAKVIGRTVESLLLLNYPKSKMELIVINDNSSDNSKEILEN
                     IKDRYNNYNFTIINTDSLTGGKGKSNALNIGYKVSKGDFIAVYDADNTPDKNALRYLV
                     QTIVMNDELGAVIGKFRTRNKNKNLLTKFINIETLSFQWMSQAGRWQLFNLCTIPGTN
                     FILRRSIIEEIEGWDSKAIAEDTEISFRIYKLGYKIKLVPQSITWEQEPETVKVWIKQ
                     RTRWAKGNIYVLMKYIKNIFKQGRNKIVFDIAYFFSVYFLFLTTVIISDILCVLSISK
                     LVEISIPINFFLIWILSYLLFIIEVSISLTIEKGEATIENIFIVAIMYFTYSQLWLFV
                     AIKGMIEYLKDIIFKREVKWYKTERF"
BASE COUNT         1142 a          542 c          312 g         1409 t
ORIGIN      
        1 tgataatatc atgactacaa ttatagtaat aaaagaaatc attaaaaata ctttagttgt
       61 cttatttata ctcatattcc agcttgaatc ttttacttct ttcttaggtt ttaaattata
      121 agctaaatct tctacctctc cgtattcatc aattatcaat gtatctcctt ttaaatcatt
      181 aactttactt ttattcaaca aataatccat tcctaaatat aagttttttt cattcattga
      241 actaattacc atgatatttt tttgattatt atatggtgat tttattaatt gaatggtaga
      301 aatatttttt ccataatcat caataaagct tatcttatca tttgatataa aattggagta
      361 atttttatcg aactttatat ttaaattatt atttaacatc tttataacag aattattatg
      421 tggcactcca aaaactacaa tattagtatc cttatacttg tcactaaatt cttttcctct
      481 aattacattt atatttccat tatgtgaatt tatattcgta cctaaagtaa cacctaatct
      541 aaacatccag gtcatagctt gtgaccctga ataatctggc attatcacag ttaaatcatt
      601 aaactcatca tctctaacaa atggatacgg ataattttca aaagataagg tttcattttc
      661 tttcgtagat aacgccaaat aagaattatt tgatacatat gcccaaggat tattactctc
      721 tctagtaaca caattcgaat tttttatact caaattaaat gtcaatttaa cttgataata
      781 gtttttatta tctatatctt taggtatttt taattctaac ttatcattat tagaatgtga
      841 cctatccaat tttttacttc ccacaataac atcatttata gacactgtaa ctaaagattt
      901 ttcaaaatct aaattgtcag aatatcttaa atttaagata atctttgaac catcatctaa
      961 taccttaccc gttggtattt tgacatcaaa caaagcttgt tgattaaatg ctccttccaa
     1021 caaaaagtca gaatatccta agtcttttaa tgttaaatga cctaaattta atttttgttc
     1081 cctattaatt tttatattag tctctttgtt tacaagcact gatgatgata aaacttggtt
     1141 agaaagtcta ttttctatta ataaatttga tgcctttatt aaatcatctt cattactacc
     1201 aattaccacc atcatcttct tattcttatt gtatggtgaa tctacttgct tgattataca
     1261 gtttgaagat aatagggttt gttcctttat acttaaaata tccaaaattt cttctgctgt
     1321 attttcaggt ttaccaatat atataatatt atcatctgac caatttttca tttctgaata
     1381 taatttaaca tctaacttta aatcatcatt ttttgttatt tttccaaatg atgatgccaa
     1441 attaaacact gctgtagtct ctcctctagt catattatct ggtactacta tagttgtatc
     1501 taatttatga ttattttcaa tttctgcata tggatacgga tattcattta ttgagttttc
     1561 cacctttttc tgcttatatc taattgatat atatgattct ttgtgaataa ccatccagtt
     1621 cccagtattg gaatcatctt ggcaaatttt atcagatatt gttttgtatg tttttatttt
     1681 tatttcatta tatccttgaa ttatataatc ttttggaact aatacttcta atgtattttt
     1741 ataatttgtc ttagcattta atttaataga ttttattggt acattattta tcaatacagt
     1801 taaacttgaa acatctccat ttaatatttg gctcttacta aaattaagat gcaataaaac
     1861 ttcttcaata tcccaatttt tatttacttc aaaaaaagta ctattacttc ctataacacc
     1921 atcaatagtt atatctcttt caaacttata atttttaact ttagtctcat cagcatttac
     1981 tttgtatata agtgatatat ttgaaaaaaa caatactaaa gatataattg atataataaa
     2041 ttttttcatg ttaattcctc ctgtacatga atcaaaatct ttctgtttta taccatttaa
     2101 cctctctttt aaaaattata tcttttagat actctatcat cccttttata gctacaaaca
     2161 gccatagttg agagtatgta aaatacatta ttgcaactat gaatatattt tcaattgttg
     2221 cttcaccttt ttctatagta aggcttatac tcacttctat aataaataaa agataagata
     2281 gtatccatat tagaaaaaag ttaattggaa tagaaatttc tactagttta gatatactta
     2341 aaacacataa aatatcggat ataattacag ttgttaaaaa tagaaaatat acagaaaaaa
     2401 agtaagctat atcaaacaca atcttatttc ttccctgttt aaatatattt tttatatatt
     2461 tcattaatac atatatattc ccttttgccc atctagtacg ttgctttatc cacaccttaa
     2521 cagtttctgg ctcttgttcc catgttatag attgtggtac aagctttatc ttgtatccca
     2581 atttatatat cctaaaactt atttctgtat cttctgcaat tgctttacta tcccatcctt
     2641 caatttcttc tatgatactt ctcctcaata taaagttagt tcctggtatt gtacatagat
     2701 taaaaagttg ccatctacca gcttgtgaca tccattgaaa gcttaatgtt tctatgttta
     2761 taaatttggt taataggttt ttatttttat ttcgagtcct aaatttccca ataacagcac
     2821 ctaattcatc attcattact atagtttgta ctagatatct aagtgcattt ttatctggtg
     2881 tgttatctgc atcataaaca gctataaaat ctccttttga aaccttatat ccaatattta
     2941 atgcattaga ctttcctttt ccacctgtta aactatctgt atttattata gtgaaattat
     3001 aattgttgta cctatccttt atattttcta aaatttcttt actattatca gaagaattgt
     3061 catttataac aataagttcc atcttactct taggataatt gagtaataac aaagactcca
     3121 cagttctacc tatcacttta gcttcattat gagcaggaac aagtattgat atcataggat
     3181 attcatcaat ttcttttatt tctttatcaa agtttttaaa ataaaatata tatccaccca
     3241 tagctaaaat aatatttata agcaataaag accatatact aaacagacta aatacgaaca
     3301 aatactctga tacatttaaa tctctcataa atttctccta aaatatttta aaatattgat
     3361 tctaatataa aatcatttat tttaaaaatt tctttttatc tattc
//
LOCUS       DAMJWP010000287         3381 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.294, whole genome shotgun sequence.
ACCESSION   DAMJWP010000287 DAMJWP010000000
VERSION     DAMJWP010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.294"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(213..944)
                     /locus_tag="PC700_003404"
     CDS             complement(213..944)
                     /locus_tag="PC700_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582670.1"
                     /translation="MNIFDILRDYLLVQVDKYNLNMDMISIVSKSLSSKEAIGNTKRK
                     DFPIIVGKEIMLEADFKGAKGQAFTSTPSTFEGSLKDILSLDLHDNPHDRSLFIASLN
                     AVMKYLGKTDRTIHCKNNEPEVCAKKFPEFIKMEFGNPKVAIIGYQPAIIDNIKDFFE
                     TRVLDLNPEFVDTIQYNVKIEDGIRDYEDVISWADLVICTGSTLCNNSIINFLSLNKP
                     VYYYGTTIAGASNILGLKRLCFCSK"
     gene            complement(931..1626)
                     /locus_tag="PC700_003405"
     CDS             complement(931..1626)
                     /locus_tag="PC700_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2582671.1"
                     /translation="MLKIKNLSLDLPNFKLQENSLNINNHEYFVLLGASGSGKTLFLE
                     TLAGRYPSAKGNVSYKENLISNLPPENRNIGFVYQNFELFPNMNVEDNIRFPLKLRKI
                     PIDEQILKANHLSKLMQIEDIKNRYPKNLSGGEKQRVAFARALIAEPDILLLDEPMSS
                     LDYITKKHISKILKDVYIKYKPTVIHVTHDISEAMFFAHKIGIMNNGKINHIFDLNEE
                     IKNKGESFFYEYI"
     gene            complement(1628..2419)
                     /locus_tag="PC700_003406"
     CDS             complement(1628..2419)
                     /locus_tag="PC700_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2582672.1"
                     /translation="MKNKFFTTLLTGFFAIVFFFIVSPLLMLIIKGISYVPICLKSVE
                     VQYAITLSIKTSLISTIVCLLLAIPVAYFLHITKLPFKKLIIQIINLPMSLPHLVSGI
                     ALLLLFGRMGIGDSIYKIFKLDFIFTKQGIVLAQVFVNLPLTIKILHTSLNESNEKMI
                     FVARTLGCNSWEAFRFIILPNLKTGIISATVMTWSRALGEFGAVAMIAGSTRMKTEII
                     PTSIYLNMSTGDIDIAIGIAVILIFISLTCLMLFEIFFNREVDKN"
     gene            complement(2429..3241)
                     /gene="modA"
                     /locus_tag="PC700_003407"
     CDS             complement(2429..3241)
                     /gene="modA"
                     /locus_tag="PC700_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2582673.1"
                     /translation="MFKKLSVLALAMIISLSITACNSKENKANDESNTKVTTADDSKE
                     KETLFVYSGAGLKKPMDEIAKKFEKENNVKVEYSYAGSAQLIAQIETSHKGDVFIVGS
                     EPIYSKAFEKELVGEHKTVAHHTPAIVVPKGNPANIQKLEDLNKDGVKLILGDKESNA
                     IGKTTQKILEKNNLKSINDNVVSTAATVNEMIVQLTSSKADATIATKDSVFGNEDVEV
                     IEIAQDKNIDQLISAGIVNYSDKKDLANKFINYIESDEAKQIFKKYGFEPVK"
BASE COUNT         1131 a          500 c          433 g         1317 t
ORIGIN      
        1 ttttttttat tagtttaaaa taaagaaaac atttatgtat aagactaatc aaaatattaa
       61 taattgatag gaattgagat ttaaagggat tgtacattta ttcgggattc attttaatag
      121 aattttactt tagtacttag aataaaagta ataacttttt aaatttaaag agtacacata
      181 tatatttgtg tactccttga tgatatgatt gcttatttac tacaaaaaca gagtcttttt
      241 aaacctaaaa tatttgatgc accagctatc gtagttccat aatagtaaac tggtttgtta
      301 agtgatagaa aatttataat tgagttatta cataatgtac ttcctgtgca aatcactaaa
      361 tcagcccaag atattacatc ttcataatct cgtataccat cttcaatctt aacattatat
      421 tgaatagtat ccacaaattc aggatttaaa tctaatactc ttgtttcgaa aaagtcctta
      481 atattatcaa ttatggctgg ttgatatcct ataattgcta cttttggatt accaaattcc
      541 atttttataa actcagggaa tttttttgca catacctctg gctcgttatt tttacaatga
      601 atggttctat ccgtcttgcc taagtatttc ataactgcat ttaatgatgc tataaaaaga
      661 cttctatcat gaggattgtc atgtaaatct aatgataata tgtcttttaa agaaccttca
      721 aatgtagatg gagtagaagt aaatgcttgt cctttagctc ctttaaagtc agcttcaagc
      781 attatttctt taccaactat aattgggaaa tctttacgct tagtatttcc aattgcttct
      841 tttgaagata gagatttgga cacaatactt atcatatcca tatttaaatt atatttatcg
      901 acttgtacta atagataatc tctcaaaata tcaaatatat tcatagaaaa aagattctcc
      961 tttatttttt atttcttcat ttaaatcaaa aatatgattt atttttccat tgttcattat
     1021 tcctatctta tgagcaaaaa acattgcttc agaaatatca tgagtaacat gaatcactgt
     1081 tggtttgtat tttatgtaga catccttcaa aattttgctt atatgttttt ttgtaatata
     1141 atccaatgaa ctcataggtt catctaaaag taatatatca ggttcagcta ttaaggcacg
     1201 tgcaaaagca actcgttgct tttcgcctcc acttaaattt ttaggatacc tattttttat
     1261 atcttctatc tgcattaatt tagacaagtg gttagcctta agaatctgtt catctatagg
     1321 tatttttctt aatttaagtg gaaaccttat attatcttca acattcatat ttggaaataa
     1381 ttcaaaattt tgatatacaa atcctatatt tctattttct ggaggtaagt ttgatattaa
     1441 attttctttg tatgatacat ttcctttagc agatggatat cttcctgcta atgtttctag
     1501 gaatagagtt ttacctgacc cactagctcc taataaaaca aagtattcat gattattaat
     1561 atttaaacta ttttcttgta atttaaaatt aggtaaatca agagataaat tttttatttt
     1621 taacatatta attcttatca acctctctat taaaaaatat ctcaaaaagc atcaagcatg
     1681 taagtgagat aaatatcaaa attacagcta ttccgattgc aatgtctata tcaccagtag
     1741 acatatttaa atatatagat gtaggaataa tttcagtttt catacgtgta gaacctgcta
     1801 tcatagctac tgcaccaaat tcaccaagtg ctcttgacca agtcattaca gttgcagata
     1861 tgattccagt ttttagattt ggaagaatta taaatctaaa tgcttcccaa ctattacatc
     1921 caagggtacg tgcaacaaat atcatctttt cattactttc atttagtgaa gtgtgaagta
     1981 ttttaatagt aagtggaaga tttacaaata cttgtgctaa aactatacct tgctttgtaa
     2041 atataaaatc aagcttaaaa atcttataaa tggaatctcc aatccccatt cttccaaaca
     2101 aaagtaaaag agcaattcct gatactaagt gtggaagaga catagggaga tttattatct
     2161 gtataatcag ttttttaaat ggtaatttag taatatgtaa aaaatatgct actggaatag
     2221 ctaataaaag gcatactata gtagatatta aagatgtttt aatactaagt gttattgcat
     2281 actgtacttc cacggatttt aagcatatag gaacataact tatcccttta atgattaaca
     2341 taagaagtgg tgatacaata aaaaaaaaga ctatagcaaa aaatccagta agtagtgtag
     2401 taaaaaattt atttttcaag tttaatcact acttaacagg ttcaaaacca tattttttaa
     2461 atatctgttt agcttcatct gactctatat aatttataaa tttattagct aaatcttttt
     2521 tatcactata gtttacgatt cctgctgata tgagttggtc tatattttta tcttgggcaa
     2581 tttctataac ttctacatct tcattgccaa agaccgaatc ttttgtggct attgtagcat
     2641 cagctttact tgatgttaac tgtacaatca tttcatttac agttgcagct gtagagacaa
     2701 cattatcatt tatagatttt aaattatttt tttcaagtat tttttgtgta gtcttaccta
     2761 tagcattaga ctctttgtct cccaaaataa gtttaactcc atctttattt aagtcttcca
     2821 atttttgaat attagctgga tttcctttag gtacaactat tgcaggtgta tgatgtgcaa
     2881 ccgttttatg ttcacctaca agttcttttt caaaggcctt tgaataaatt ggttcagaac
     2941 caacaataaa tacgtctcct ttgtgacttg tctcaatttg agctattagc tgtgcagaac
     3001 ctgcatagct atattcaact ttgacattat tttctttctc aaattttttt gctatttcat
     3061 ccattggttt ctttaaacct gcccctgaat agacaaaaag agtctctttt tcttttgaat
     3121 catcagctgt tgtcacttta gtattagatt catcattagc tttgttttct ttagaattac
     3181 atgcagtaat acttaatgaa ataatcatag ctaaagctag tactgaaagt tttttaaaca
     3241 taagatattc ccccttagta aattagtgta aattcttttt tagtatatca tactaagaaa
     3301 acgatgtcta agtttttgtt atgttttata atgttttgtt atgttttatt atgtatagtt
     3361 aagaaaaaat ctatttataa t
//
LOCUS       DAMJWP010000288         3346 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.295, whole genome shotgun sequence.
ACCESSION   DAMJWP010000288 DAMJWP010000000
VERSION     DAMJWP010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.295"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            44..775
                     /locus_tag="PC700_003408"
     CDS             44..775
                     /locus_tag="PC700_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDF2582674.1"
                     /translation="MNIYAHRGFSGKYPENTILAFKKCLYMDIYGIELDVHRTKDGKI
                     VVIHDEKVDRTFNGHGFVKDFTLRKLKTLNSSFEGYQNNKECKIPTLEEVLILISPTD
                     LILNIELKTDKINYPNIEKDVLELILKYNMKNRVLISSFNSNSLKNFHKLDPSVKTGL
                     LCYLPINNVVNFAKFLGNSYLHPPLVLVNESLIELCHKNLLGVNVYTVNEEDDILHCL
                     KLNVDGIFTNYPDIASNLLHSKQYS"
     gene            855..1490
                     /locus_tag="PC700_003409"
     CDS             855..1490
                     /locus_tag="PC700_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDF2582675.1"
                     /translation="MDNLLNELEALKSNLDNLPIEKIENYDLSKTALFIIDVNNGFAR
                     QGALYSPRVESLIKPIEMFTKKISNKLNRVIAFTDSHTPKSIELLSYPVHCLENDVES
                     ELVDELKSIENLQILPKNSTNGFFALENLDFDNIDNIIIVGDCTDICIYQFAITLKSY
                     FNQHNIEKNIVVPMNLVDTYDIPNIHPAEILNLVFFNSMIQNGVNVLKEIR"
     gene            complement(1741..2334)
                     /locus_tag="PC700_003410"
     CDS             complement(1741..2334)
                     /locus_tag="PC700_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3867 domain-containing protein"
                     /protein_id="HDF2582676.1"
                     /translation="MSDDRIIDFNELKNKVKDSDVDKFEQYIYNLYFSVMDGKMSMAE
                     FSRKIFDYMRDNNISQEKFMKIQKQFMERYGMDTEEVEKQLRNFGIDPSTAGFMSNNT
                     SSKVSTEDLESFKKSAGFYEKYGEKIQPKSCITTFIKNDLNDINVIIDQEKIMLCSDR
                     KINLMDSELNEFLLEYKNMFNKKIKVVMCETTNKYDY"
     gene            complement(2405..3268)
                     /locus_tag="PC700_003411"
     CDS             complement(2405..3268)
                     /locus_tag="PC700_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HDF2582677.1"
                     /translation="MLGIIAPSGPLRNTSLEEIKFNLESYGYEVKFSESCSLNYKGYL
                     AGNDDIRARDIEDMFLDKDVDIIMCLRGGYGTTRILDKINYDIIKQNPKPFIGFSDIT
                     GLNLAFYKNCGLLPYHGIMAANVGKWDKFTYKSLVNALEFKDDLYLENPKEEKIYTVC
                     EGKAEGIIMGGNLSLIIATMGTKYEIDAKDKILFIEEIGEPQYKLDRMLTQLYSSGKL
                     EECNGIIFGDFKDCIEENDLMELLIEFANKVNKPSIYNLQSGHCIPMITIPLGRMCEL
                     DATEKIVKLKK"
BASE COUNT         1185 a          503 c          363 g         1295 t
ORIGIN      
        1 atttttataa gataagttat acttatagga gggaaccata taaatgaata tttatgctca
       61 tagggggttt agtggaaagt atccagaaaa tactattcta gcttttaaaa aatgtttgta
      121 tatggatata tatggaatag aacttgatgt ccacagaact aaggatggaa aaattgtagt
      181 tattcatgat gaaaaggttg atagaacttt taatggacat ggttttgtaa aagactttac
      241 tttaagaaaa ttaaaaactc taaactcatc ttttgaagga tatcaaaaca ataaagaatg
      301 taaaattcca actttagaag aagtattaat tctaatttca cctacagacc ttattttaaa
      361 tatagaacta aaaactgaca aaataaacta tcccaatata gaaaaagacg ttttagagct
      421 tattttaaag tataatatga aaaatagagt tcttatatct agtttcaaca gcaatagctt
      481 aaaaaacttt cataaattag accctagtgt aaaaacagga ttattgtgtt atttacctat
      541 aaacaatgta gtcaattttg ctaaatttct gggtaatagt taccttcatc cacctctagt
      601 tttagtaaat gaatctctaa ttgaactttg tcacaaaaat ctacttggtg tcaatgtata
      661 tactgtaaat gaagaggatg atatactaca ctgtctaaaa ctcaatgtag atggtatttt
      721 tactaactat ccagatattg cttcaaatct tttacactca aaacaataca gctaattata
      781 caatagtttg tactccttga cttttatata attaatatgt acaattttat taaatttatc
      841 ttggaggtat aactatggat aacttattaa atgaacttga agctttaaag agtaatttag
      901 ataatctacc aatagaaaaa atagaaaatt atgatttgag taaaactgct ttatttataa
      961 ttgatgttaa taatggcttt gcaagacaag gtgcattata ctctcctaga gtagagtctt
     1021 taataaaacc aattgaaatg tttacaaaaa aaatatcaaa caaattaaat agagtaatag
     1081 catttactga ttctcatact cctaaatcaa tagaactttt gagttatcct gttcactgtt
     1141 tggaaaatga tgtagaaagt gaattggttg atgaacttaa gtcgatagaa aacctacaaa
     1201 ttcttcctaa aaattctact aatggatttt ttgcattaga aaatttggat tttgataata
     1261 tagataatat aattatagtt ggcgattgca cagatatatg catatatcaa tttgcaataa
     1321 ctctaaaatc atattttaat caacataata ttgaaaaaaa tatagttgta cctatgaatt
     1381 tagtagatac ttatgatatt cctaacattc accctgctga gattttaaac ttagtatttt
     1441 tcaacagtat gattcaaaat ggagttaatg tattaaaaga gattcgttaa gcctctaata
     1501 ctttacatcc catataatac aattatcaga tatttatttt taataaaatt tctaatagaa
     1561 aaacacagtt ttagtgagaa gattacaatt taaaacacta tctattatga ctaatcttca
     1621 caatataaaa aatcgcagtt tagatattcc ttatacaaat ttataaggaa taataaattg
     1681 cgatttttat ttttaagtag tacaattaat tgatactact tcagtattta aattcatttt
     1741 ttaataatca tacttattcg tagtttcgca cattactacc tttatttttt tattaaacat
     1801 attcttatat tctaataaaa actcatttag ttctgaatcc ataagattta tttttctatc
     1861 actacaaagc attatctttt cttggtcgat tattacatta atatcattta agtcattttt
     1921 tataaatgtt gttatacaac tctttggttg gattttttct ccatatttct cataaaatcc
     1981 tgcactcttc ttaaatgact ctaaatcttc tgtagatact ttgctggaag tattattact
     2041 cataaaacct gctgtagaag ggtcgattcc aaagtttcta agttgttttt ctacttcttc
     2101 agtgtccata ccatatctct ccataaattg cttttgtatt ttcataaatt tttcttgaga
     2161 tatgttgtta tctctcatat aatcgaatat ttttcttgaa aactcagcca tggacatctt
     2221 tccatccata acagaaaaat ataagttata tatgtattgc tcaaatttat ctacatcaga
     2281 atctttaact ttgttcttta gttcgttaaa gtctattatt ctatcatcag acatttttca
     2341 tcctcctagc taaattttta cttgtataag attataccat atttctatta tatttatata
     2401 cacactattt ttttaattta acgatttttt ctgtagcatc taattcgcac attctgccca
     2461 aaggaatagt tatcattggg atacaatgtc ctgactgaag attatatata gaaggtttat
     2521 tgaccttatt tgcaaattct attagtaatt ccatcaaatc attttcttca atacaatcct
     2581 taaaatctcc aaaaattata ccattacatt cctcaagttt tcctgaagaa tatagttgtg
     2641 ttaacattct atccagttta tattgaggtt ctccaatttc ctcaataaac aaaattttat
     2701 cttttgcatc tatttcatat tttgttccca ttgtggcaat tattaaggat aaatttcctc
     2761 ccattattat tccctctgct ttaccttcac atacagtata aatcttttct tcttttggat
     2821 tttctaaata taagtcatct ttaaattcta aagcatttac caatgattta taggtaaatt
     2881 tatcccattt acctacattt gctgccatta ttccatgata tggtaaaagt ccacaatttt
     2941 tataaaaagc taaatttaaa cctgttatat cagaaaaacc tataaatggt tttggatttt
     3001 gctttattat atcataattt attttatcca atattcttgt tgttccataa cctcctctta
     3061 agcacataat tatatctaca tctttatcta aaaacatatc ttctatatcc cttgctctaa
     3121 tatcatcatt accagctaaa taacctttat aatttaaact acaactttca gaaaacttaa
     3181 cttcgtaacc ataactttct aaattaaact ttatttcctc caaacttgta tttcttaatg
     3241 gcccagatgg cgcaattatt cctaacattt aaacacctct tcttataaaa tgatatattc
     3301 ttctccatac taaatatctc taatataatt ataagatatt ttattt
//
LOCUS       DAMJWP010000289         3328 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.296, whole genome shotgun sequence.
ACCESSION   DAMJWP010000289 DAMJWP010000000
VERSION     DAMJWP010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.296"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            960..1721
                     /locus_tag="PC700_003412"
     CDS             960..1721
                     /locus_tag="PC700_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896901.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HDF2582678.1"
                     /translation="MIGIYFSGTGNSRYCVEKFLQEYDITANSYSIEDNELLQHINNH
                     ENIIFSYPVQYSNVPKILKDFIINHSTLWNGKRVFIIATMALFSGDGSGVLARILKKY
                     GAITVGGLHVKMPDSIGDEKVLKHSLEYNKKLVIKAEKKVTKAAKKLKHGSPPKEGLG
                     FLSHIVGLFGQRLYFINKTNMYTDKLKINTEECIVCGKCVNLCPMKNLVIKNCMIVAN
                     GQCTMCYRCVNNCAKKAITLLGKKVVEQSNIKKYM"
     gene            1786..2568
                     /locus_tag="PC700_003413"
                     /pseudo
     CDS             1786..2568
                     /locus_tag="PC700_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430254.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
     gene            2835..3254
                     /locus_tag="PC700_003414"
     CDS             2835..3254
                     /locus_tag="PC700_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HDF2582679.1"
                     /translation="MDKISGKLTVLFEEPFWVGIFERQDGKKYEACRVVFGAEPKEVE
                     VYEFILERFFSLDFGSIKLEKNVTKDNIGYKRMQRKVKKEQEKETIGTKAQNALKLQY
                     EERKQDRKNLAKNRKEEEKERIFNLKQEKRKAKHKGH"
BASE COUNT         1347 a          323 c          572 g         1086 t
ORIGIN      
        1 ttatgttatt tttagtacat tacaaatttt cttatataaa aatcaatact tgaattatta
       61 tttttatgtg aaatttatct atagtatatt tttataaaca agtaatgtta ttaaaattaa
      121 gtatatagtt aaaagtgtat agcaaaaagg ctgttatatt aatgttagag gttcagggaa
      181 aatatataaa gcaaagatat ttacagataa tgtagattgc acagctataa gttagataat
      241 agaattatgt aatcaagaat ttacaacaaa gtataagata aggataatgc taaattgcca
      301 tacaggtgct agatgtgtaa atggatttaa ttaatactat tgataaagtg caacaagcac
      361 ataaaccaat tgatgagatt attaaaacta taagagatat agttgacata attaaaatat
      421 tggaacctat ttataatttt aaatcaaatc aataatgatt aagattaaaa taatattaag
      481 gtatcaatgt tataagagta caatataagg agtactatat agtactcctt acttatacat
      541 attaggcaat aggaaatctt ttcatagtta aattttcaaa tattttttag ggaactttat
      601 aattgagaat taagggtata gtatgtgatg aacttactaa atttataatt aggttatata
      661 atggaataat catcaatagt tttactttag ggaaaatata tgcttaagga agtatagctt
      721 actaatatta ttaaaatgta tatttaaaat gataaaaaaa gaattagaca agtatttagt
      781 aaattgatta tgtaaaattc tatcaagcag gaaccctatg actttagtca tgggaggttc
      841 agtaggtaac ttgttgaatt aagtttatat tatttaaata aactacttaa tttagataat
      901 ataaaaagtc agaattttaa gaatagagct atataatctt agctattagg agaagtacta
      961 tgataggaat atattttagt ggaacaggaa attcaagata ctgtgtagaa aaatttttac
     1021 aagaatatga tataacagca aattcttatt ctattgaaga taatgaactt ttacagcaca
     1081 tcaataatca tgagaatatt atttttagtt atcctgtgca atatagtaat gtgccaaaaa
     1141 tattgaaaga ttttataata aatcattcaa ctttgtggaa tggtaaacgt gtttttatta
     1201 ttgcaactat ggctctattt agtggagatg gctctggtgt gttagctaga atattaaaaa
     1261 aatacggtgc tataactgtt ggtggattac atgtgaagat gccagatagt ataggagatg
     1321 aaaaggtatt aaaacattct ttagagtata ataaaaagct tgtaataaaa gcagaaaaga
     1381 aagtaactaa agctgctaaa aaactgaaac atgggtcacc accaaaagaa gggttaggtt
     1441 ttttgagtca tattgtaggg ttatttggac aacgattgta ttttatcaat aaaacaaata
     1501 tgtatacaga taaactaaaa attaatactg aggaatgtat tgtatgtggt aaatgtgtta
     1561 atttatgtcc aatgaagaat ctagtaatta aaaattgtat gattgttgca aatgggcaat
     1621 gtacaatgtg ttatcgttgt gtaaataatt gtgcaaaaaa agcaattaca ttgttaggta
     1681 aaaaagttgt tgagcagagc aatattaaaa aatatatgta agaaatttaa agttttattg
     1741 aatgtaaatt gatgtatatt ttatgttgga aagagaggtc aaataatgga atactacata
     1801 gttgattcat ttgcaactaa actatttaaa ggaaatcctg ctggagtttg tgttcttgac
     1861 agaataatac ctttagagtt aatgcagaaa atagcagaag aaaacaattt accagaaaca
     1921 gcatttgtag ttaaaggtaa aggaaattat gaattacgat ggtttactcc taaagcagaa
     1981 attgatttat gtggtcatgc aactttagca gcagcttatg taatatcaaa ttttattgat
     2041 gttaatgtaa aaaggataga tttctttact caaagtggaa agcttgaagt tacacgaaat
     2101 ggaaatctat atgaaatgat atttccagaa ataatgccta ttgaaataga gttatcacca
     2161 taacaagcta atttgatagg ctgtgtacca agtgctgtct attcttcaag agatttaatc
     2221 ttattgctaa acagtgaaca agaagttata aattataaac ctaattacgc acaattgcgt
     2281 aaattgacag actggttagg tataataatc acaccacaag gaagtaatac agattttgta
     2341 tcaagatatt tttgtccaga attagattca gaagaccctg ttactggttc atcacattgt
     2401 aattttattc catactggtc tgaaaagttg ggaaaacaca aaatggtagc tgctcaactt
     2461 tctaatcgtg gtggtataat tcagtgtgaa gtgttgaagg ataatactgt aaaaatttct
     2521 ggtgaagcag tcttgtttat gcagggaaca ataaagattg atatttaaat aagtataata
     2581 actaatttat tatgaaaaat aattaatttt cttagtgtta acaataataa agtagagtta
     2641 aaaagcttga tttcacttaa atcaatatga tagatgttga tactaagatt gacaagcatg
     2701 attaaaaaag ataatattta atctataaag ataaaaacac agtctggttg gtagtccaga
     2761 cgcagcaata gctgtcagta acctgcctcc ttggttgtcc gttctttaga aagtaattta
     2821 aggaggaact agcgatggac aaaattagtg gaaaattaac agtcttattt gaagaaccat
     2881 tttgggtagg tatatttgaa agacaagatg gtaaaaaata tgaagcatgt agggtagttt
     2941 ttggagcaga acctaaagaa gtagaagtgt atgaatttat acttgaaaga tttttctcat
     3001 tagattttgg tagcattaaa ttagaaaaaa atgtgaccaa agacaacatt ggatacaaaa
     3061 gaatgcagag aaaagtaaaa aaggaacaag aaaaagaaac tatagggact aaggctcaaa
     3121 atgctcttaa gcttcaatat gaagaaagaa aacaggatag aaaaaaccta gctaaaaata
     3181 gaaaagaaga agaaaaagaa agaatattta atttgaaaca agaaaagaga aaagctaaac
     3241 ataagggtca ttaattttaa ttaaatatct ttttaataat tgctagaaaa aggtgtataa
     3301 agtttttaat tttatacacc ttaattta
//
LOCUS       DAMJWP010000290         3305 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.297, whole genome shotgun sequence.
ACCESSION   DAMJWP010000290 DAMJWP010000000
VERSION     DAMJWP010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.297"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            191..838
                     /locus_tag="PC700_003415"
     CDS             191..838
                     /locus_tag="PC700_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HDF2582680.1"
                     /translation="MIRQTPLEKRYDKLGPHIVTAFKKRYFDAYYCKTRNEALQQILD
                     LIPQNHVVSWGGSETLKEMGVQTAVREKGFKVIDRDLAKTPEERTEIMRQALLCDTFL
                     MSSNAISEDGQLFNIDGNGNRVAAMIFGPKNVIVVAGMNKIVKNIDDAIQRTRTIASP
                     AVVQRFQDVNTPCYINGSCIDCTSPESICAYMVTTRISRPAKKIKVILVGENLGL"
     gene            complement(1005..1574)
                     /locus_tag="PC700_003416"
     CDS             complement(1005..1574)
                     /locus_tag="PC700_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582681.1"
                     /translation="MKKFILCIIMFAISVLVLAGCTNDNKIKNEVDSMFNSIKTQDAD
                     TVDKYFPKSGLGNLIYEDKEGFKMYSKNMTWEISDIKNNKDKVTVDLKINNTDMVSIL
                     KKNPPKLGEMMPNPTMKDIDSAKKKEFNITLELVKSGDNYVCKVNQESAMKLLNVITG
                     GGIDYLADYNKDYYKQLDEEQKNYENKNS"
     gene            complement(1579..2478)
                     /locus_tag="PC700_003417"
     CDS             complement(1579..2478)
                     /locus_tag="PC700_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2582682.1"
                     /translation="MKLLSKVNNNIRLIIQVAFTALTNGYVNGFLEGTIYGGESKKLC
                     VPGLNCYSCPGALGACPIGSLQAVLSSREYKFSFYVVGFLMAFGAFFGRFVCGWLCPF
                     GLVQDLLHKVPFPVKIKKVYGDKYLKYLKYIVLIMFVIILPVAIVNVAGGGNPWFCKW
                     ICPSGTLLGGIPLILGNENLRESIGFLFSWKLSILLLILLMSIITYRPFCKYICPLGA
                     IYGFFNPISIYRFKIDKDKCTNCTACQTKCKLDIPIYKDVNSPDCIRCGECIKVCPQN
                     AITTTFSKDKKENNICAKKYRGI"
     gene            complement(2489..2638)
                     /locus_tag="PC700_003418"
     CDS             complement(2489..2638)
                     /locus_tag="PC700_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582683.1"
                     /translation="MSNFIKDNKKGISIIAISIIFIALGTYRGETNTVLTKAIMICLE
                     CIGIG"
     gene            complement(2631..3215)
                     /locus_tag="PC700_003419"
     CDS             complement(2631..3215)
                     /locus_tag="PC700_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HDF2582684.1"
                     /translation="MKKFFMVVLGCIFVLGVISGCSKKESKPVEKNKSNSEWFSNLDT
                     EDVDGNKVTKEIFSNKELTLINVWTTWCGPCVGEMPELEALSKEYESNNSNVAIKGLV
                     VEVDKTDMRTGLSNEEKNLVKNIMKKSDATYQQLTVSEELKKTDFKRVMEFPTTYFVD
                     KKGNFVGEKVVGANSKEEWKKIIDERLKMVKANE"
BASE COUNT         1158 a          564 c          369 g         1214 t
ORIGIN      
        1 agttatttgt taaaaactaa tagtaaatct tatactaatc gcatatttat gttataacta
       61 tattgtaact attttcgtaa tcttaggaaa agattactta taaaattaac cttatacaat
      121 aatatatcgt ataatcttaa ttagcataat agttacaata ttaaaattta aatatgggag
      181 tgattttagt atgataagac aaacaccatt agagaaacga tatgacaagc taggtcctca
      241 tattgtaaca gcattcaaaa aacgttattt tgatgcttat tactgtaaaa ctagaaatga
      301 agctttgcaa caaattttag atttaattcc tcaaaatcat gtagtatcat ggggaggatc
      361 tgaaacactt aaagaaatgg gcgtacaaac tgctgttaga gagaaaggat ttaaagttat
      421 tgatagagat ttagcaaaaa ctccagaaga aagaactgaa attatgcgtc aagcacttct
      481 atgcgatact tttttgatga gtagcaatgc aattagtgaa gatggacaac tctttaatat
      541 tgatggaaat ggcaatcgtg tagctgcaat gatatttggt ccaaaaaatg ttatagttgt
      601 agctggtatg aataaaattg taaaaaacat tgatgatgca atacaaagaa ctagaacaat
      661 tgcatctcca gcagttgttc agcgttttca agatgtcaat acaccatgct atattaatgg
      721 aagttgtata gattgcacaa gtcctgaaag catttgtgca tacatggtta ctacaagaat
      781 cagtcgtcca gcaaaaaaaa taaaggtaat attggtagga gaaaatcttg gtctataaaa
      841 tatacataca tgtaaatagc tctataaata ataaaatcat atgaaataac ttatttctaa
      901 aagtggtaga ataaaagctt ctacaagact ttcattctac cacttttata ttcttttaat
      961 ttgtgtttct atattattaa ttgctccaat tttcatatat attactaact atttttattt
     1021 tcataatttt tttgttcttc atcaagctgt ttataataat ctttattata gtcagctaga
     1081 taatctatac cacctcctgt aatcacattt aataatttca ttgcagattc ttgattaact
     1141 ttacaaacat aattatcacc agatttaaca agctctaatg taatattaaa ttcttttttc
     1201 tttgcagaat caatatcttt catagttgga tttggcatca tttctcctag tttaggagga
     1261 ttctttttta aaatagaaac catatcagta ttgttaattt ttaaatctac agtcacttta
     1321 tccttattat tctttatatc tgaaatttcc caagtcatat ttttagaata cattttaaat
     1381 ccttctttat cttcgtatat aagattacct aaccctgatt ttgggaaata cttatcaaca
     1441 gtatctgcat cttgtgtttt tattgaatta aacatactat ccacttcatt ttttatctta
     1501 ttatcattag tacaaccagc taacaccagt actgatattg caaacattat aatacataga
     1561 ataaatttct tcattaaatt atatccccct atactttttt gcacaaatat tattctcttt
     1621 tttatctttt gaaaaagttg tagtaattgc attttgtgga caaactttta tacattcacc
     1681 acacctaata cagtctggac tatttacatc tttatatatt ggtatatcaa gcttacattt
     1741 tgtttgacat gctgtacaat ttgtacattt atctttatca attttaaatc tatatattga
     1801 aataggatta aaaaaaccat agattgctcc taaagggcat atatacttgc aaaatggcct
     1861 ataagtaatg attgacatca ataatatcaa tagcaaaata cttaacttcc agctaaataa
     1921 aaatcctatt gattctctta aattctcatt tcctaaaata agtggtattc ctcccaaaag
     1981 tgttcctgaa gggcaaatcc atttacaaaa ccaaggattt ccaccccctg ctacatttac
     2041 aatagcaact ggcaaaataa ttacaaacat tattaagact atatacttta gatattttag
     2101 atatttatca ccataaactt tttttatctt tactggaaag ggtactttat gtagtaaatc
     2161 ttgtacaagt ccaaagggac aaagccaacc acaaacaaat ctgccaaaaa aagcaccaaa
     2221 tgccattaaa aaaccaacaa cataaaatga gaatttatac tctctgcttg ataaaactgc
     2281 ttgtaatgaa cctattggac atgctccaag tgcacctgga catgaataac aattcaatcc
     2341 aggtacacat aattttttac tttctcctcc atatatagtt ccttctaaaa atccatttac
     2401 atatccattt gttaatgctg taaatgcaac ttgaataata agtcttatat tattatttac
     2461 cttagataat aacttcattc ctcttctcct aaccaatacc aatacactca agacaaatca
     2521 taattgcttt agttaatact gtatttgttt ctccacgata tgtacctaat gcaataaata
     2581 taatactaat tgcaataata gaaatgcctt ttttattatc tttaataaag ttactcattg
     2641 gcttttacca tttttagtct ttcatctata atttttttcc actcttcctt actatttgca
     2701 cctacaacct tctcacccac aaagtttcct ttcttgtcta caaaataagt tgttggaaac
     2761 tccataactc ttttaaaatc agttttttta agttcttcag atacagtcaa ttgttggtaa
     2821 gttgcatctg actttttcat aatgttttta actaaatttt tctcttcatt tgaaagacca
     2881 gttctcatat cagttttatc aacttctaca actaatccct ttatagctac attggaatta
     2941 ttactctcat attcttttga taatgcttca agctctggca tttcacccac acatggtcca
     3001 caccaagtag tccagacatt aataagagta agttctttat ttgaaaatat ctcttttgtt
     3061 accttatttc catcaacatc ctccgtatct aaattactaa accattcact attactcttg
     3121 tttttttcta ctggcttaga ttcttttttg ctacatccag aaatcacacc taaaacaaaa
     3181 atacatccta aaactaccat aaaaaacttt ttcatatctc acactcctag aatttatata
     3241 gtatataaaa tggctgcttg aaataaaaat aacttctctt ctatattaaa acattatatt
     3301 taata
//
LOCUS       DAMJWP010000291         3281 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.298, whole genome shotgun sequence.
ACCESSION   DAMJWP010000291 DAMJWP010000000
VERSION     DAMJWP010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.298"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     misc_binding    169..379
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            605..1420
                     /locus_tag="PC700_003420"
     CDS             605..1420
                     /locus_tag="PC700_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDF2582685.1"
                     /translation="MKVFKKLLSLGLVLGLTLSLVGCSGGGEKTKLEQIKDNGKLVVG
                     TSAEFPPFEFHKVVDGKDSIKGFDIMLAEEFAKELGVKVEIKDMSFDGLIGALNADQV
                     DIVLAGMSPTPEREKSVDFSELYYLSRNAVIVKDADIDKVKTEDDLKKLRVGVQAGSI
                     QEEYVVNTLKMTTTKSLKAIPDLITELKNGNIDAVVTNEAVSLINVKKYDGIKMANTE
                     VGKDVTEGMAAAIKKSDNNKDFIELLNKKIKELQDGKKIEEFLNEASTEAASN"
     gene            1448..2125
                     /locus_tag="PC700_003421"
     CDS             1448..2125
                     /locus_tag="PC700_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDF2582686.1"
                     /translation="MSLDFSFLSRFGTSFLEGTGVTVSISLVALCFGFIIGIIICMAK
                     ISKSKVLRAISSIYIEVLRGTPLLVQIYIVWFGLPQLGIRFPMLFGIPSEFIASAFAL
                     SVNSGAYVAEILRSGIQSVDNGQMEASRSLGLNYWSTMRYIIIPQAIKNILPSLANEF
                     ITLVKESSIISVIGVVEIMRTADIVKNAAFRALEPLIVAAAIYFVITFTLSRLVGLLE
                     KKLSVSN"
     gene            2184..2906
                     /locus_tag="PC700_003422"
     CDS             2184..2906
                     /locus_tag="PC700_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004398740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDF2582687.1"
                     /translation="MITIKNLSKSFGDLNVLKNIDLEIAKGEIMVIVGPSGSGKSTLL
                     RCMNLLEIPTGGEIIFEGKNLVDKKTNIDEVRQNIGMVFQNFNLFPHKTILDNITLAP
                     IKLKKMTKEEAEKKAEILLSRVGLLDKKDSYPSQLSGGQKQRIAIARALAMEPDMMLF
                     DEPTSALDPEMVNEVLDVIKELAKEGMTMAIVTHEMGFAKEVADRVIFIDGGSILEDN
                     TPEEVFGNPKHERTKAFLAKVL"
BASE COUNT         1318 a          333 c          605 g         1025 t
ORIGIN      
        1 aagaaatagc taaaaatatg aaatatacta tttataatta caaaaataat atcaataaat
       61 ataaacagta gtatttagat aatttacaag taaaaattat taaaaaaata aaaaacttat
      121 tgacaaaaat attataagga agtacaattg tattaacaat ttaaggaaac aaatccaatg
      181 atagagaaag tagcatatat ggatttacag cgagttaggg gttggtgtaa gcctagcaat
      241 gaagtctatg tgaagagagc tctggaggag attatcagaa actttagtat gataattggt
      301 ttacccgcct taagggattg agaggataag tacttactta tcaaaaagag tggtaacgcg
      361 gatataattc gtctcttagc tgtaaagcta agggactttt ttgatttaaa aataaaaata
      421 tccatacagc ttaaatgcta acagtcacaa attttgaaag taatataagt atttatcaat
      481 aagagtggta acgcggtaaa gtcgtctctt agtcattaaa gactgagaga ctttttttgt
      541 aactcgaaac atttaagtat taaaatctga gggaaattat aattaaaaga gaggggtagt
      601 taaaatgaaa gtatttaaaa aattattaag tttaggatta gtattaggat tgacattatc
      661 gctagtagga tgtagtggtg gaggagaaaa aacaaagtta gaacaaataa aagacaatgg
      721 aaaattagtt gttggtacta gtgcagaatt tcctccattt gaatttcata aggtagtaga
      781 tggaaaagat tcaataaaag gattcgatat aatgttggct gaagaatttg caaaagagct
      841 tggtgtaaag gttgaaatta aggatatgtc atttgatgga ttaataggtg ctttaaatgc
      901 agaccaagtt gatattgttc ttgcaggtat gtctccaaca ccagagagag aaaagagtgt
      961 tgatttttca gaattatatt atttaagtag aaatgcagtt atagttaaag atgcagatat
     1021 agacaaagta aaaactgaag atgatttaaa gaaacttaga gttggagttc aagcaggaag
     1081 tatccaagaa gaatatgtag ttaacacttt aaaaatgaca actacaaaat ctttaaaagc
     1141 aatacctgat ttaattacag aattaaaaaa tggaaacata gatgcagttg ttactaatga
     1201 agcagtttct ttaataaatg ttaaaaaata tgatggaata aaaatggcaa atactgaagt
     1261 tggtaaagat gtaacagaag gtatggcagc agcaatcaaa aaatctgaca ataacaaaga
     1321 ttttatagaa ttattaaaca aaaagataaa agaattacaa gatggtaaga agatagaaga
     1381 atttttaaat gaagcatcta ctgaagctgc ttcaaattag taagaaagtt atgtgggaga
     1441 tgaaattatg agtttagatt ttagtttttt aagtagattt gggacttctt ttttggaagg
     1501 aacaggtgtg acagtatcaa tttctcttgt ggcattatgc tttggattta taataggtat
     1561 aattatctgt atggcaaaaa tatcaaagag caaagtatta agagcaatat catcaatcta
     1621 tattgaggtt ttaagaggga caccattatt agtacagata tatatagtat ggtttggttt
     1681 acctcaatta ggaataagat tccctatgtt gtttggtata ccaagtgaat ttatagctag
     1741 tgcatttgca ttatcagtaa actctggtgc atacgtggca gagatactta gatcaggtat
     1801 acaatcagtt gataatggac aaatggaagc aagtagatca ttaggattaa attattggtc
     1861 aactatgaga tatataataa tacctcaagc tataaaaaat atattacctt cattagcaaa
     1921 tgagtttatt acacttgtaa aagaatcttc aataatatct gtaataggag ttgttgagat
     1981 aatgcgtact gctgatatag taaaaaatgc agcatttaga gcattagagc cattaatagt
     2041 agcagcagca atttactttg ttataacatt tactctatca agattagttg gattattaga
     2101 aaagaaacta tctgtgtcta actagatgag agcatgttta gcatttaggg aaaacataga
     2161 atagtaaaaa ggaaagtgat aatatgatta caataaaaaa tttaagtaaa tcttttggag
     2221 atttaaatgt attaaaaaat atagacttag aaatcgctaa aggcgaaata atggttatag
     2281 taggtccaag tggttctggt aaaagtacac ttcttagatg tatgaattta ctagaaatac
     2341 ctacaggtgg agaaataata tttgaaggaa aaaatttggt cgataaaaaa actaatattg
     2401 atgaggttag acaaaatata ggtatggtgt ttcaaaactt taatttattc ccacataaga
     2461 ctattttaga taatataaca ttagcaccaa ttaaattaaa aaagatgact aaggaagaag
     2521 cagaaaaaaa agcagaaatt ttgttgagta gagttggtct tttagataaa aaagattcat
     2581 atccatcaca attatctgga ggacaaaagc aaagaatagc aatagctaga gcacttgcta
     2641 tggaaccaga tatgatgttg tttgatgagc caacatctgc tcttgaccca gaaatggtaa
     2701 acgaagtttt ggatgttata aaagaacttg ctaaagaagg tatgacaatg gcaattgtaa
     2761 ctcatgagat gggatttgct aaggaagttg cagatagagt tatttttata gatggaggaa
     2821 gtatacttga agataatact ccagaagaag ttttcggaaa tccaaaacat gaaagaacaa
     2881 aagcattttt agcaaaagtg ctataatata taaaaaggat gttattgata ttagaaattc
     2941 aatacatcct tttttagtga tggttaatat taattttaaa attaaagtta tattaatatt
     3001 aactttattt ttacgatttt ttgcagaaaa aaaaagagct cggggtaagc tctttttttc
     3061 caccgaatta atttccgtta ctaatccgat ttttaataaa tataaaatat atccttatga
     3121 aagggtattg ggaatagata tattaactaa ctagggttac cagggggata accttaattt
     3181 attataacaa aatgcgacac aaaaatcaat attttttttg aaataaatta taaaagttat
     3241 ttttataaca agtaagctta taaatttaac ttttatagta a
//
LOCUS       DAMJWP010000292         3195 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.299, whole genome shotgun sequence.
ACCESSION   DAMJWP010000292 DAMJWP010000000
VERSION     DAMJWP010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.299"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            168..1085
                     /gene="pfkB"
                     /locus_tag="PC700_003423"
     CDS             168..1085
                     /gene="pfkB"
                     /locus_tag="PC700_003423"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDF2582688.1"
                     /translation="MIYTVTLNPSIDYIVKLDELKTGSTNRVKEEYVYPGGKGINVSR
                     ILKELGNDNTSLGFISGFTGEYIIRTLEEKELKTDFIKIKSGFSRINVKIKALKETEI
                     NGQGPNIDDEDIDTLYKKLDKLNQDDILILAGSIPSTLDEKLYENIMARLEKKNIKVV
                     VDATKNLLLNVLKYKPFLIKPNNDELEELFGVKLNSIEDMVKYAKRLKEMGAINVLVS
                     MGKDGALLITEEDEVLISDVPKGKVKNSVGAGDSMVAGFISGYLNTGKYDYALKLGAA
                     SGSATAFSYDLAKREYIDKLVNEISVKQF"
     gene            1122..3041
                     /locus_tag="PC700_003424"
     CDS             1122..3041
                     /locus_tag="PC700_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2582689.1"
                     /translation="MKIIDLLDEKSIKLNLNSKTKSEAIEELVDLVANSGNLNDKENY
                     KKAILAREEMSTTGIGEGVAIPHAKNSSVTKACIAAAVSKEGIDYESFDGSLSNLFFM
                     IAAPDGANNTHLEVLSRLSTILMDEDFRNKLINSSSEKEFKEIIDKKEREKFSEEYQD
                     EKVAEKNIIDTKENDANKYPKVLAVTACPTGIAHTFMAAESLNKMAENKGVSIKVETN
                     GSAGVKNKLTKDEIENATCIIVAADKNVEMARFNGKKVIKTKVADGIHKAEELIDKAV
                     NGDAPIYHGGDGSHNESNEESESGFRKVYKHLMNGVSNMLPFVIGGGILIAIAFLLDD
                     YTINPSNFGSNTPIAAFFKGIGDKAFGFMLPVLAGYIAYSISDRPAFVVGFVGGALAG
                     DGGSGFLGALLAGFIAGYLVEGLKKIFSVLPASLEGIKPVLLYPLLGTLLMGIIMTFL
                     VIPPVTAINNAMVGFLNGLGGTSKIVLGLVLGGMMAVDMGGPVNKAAYVFGVASLESG
                     QFEIMAAVMAGGMVPPLAIALATTFFKNRFTKEERDSGKVNYVMGLSFVTEGAIPFAA
                     GDPLHVIPACVGGSAVAGALSMLFNAALRAPHGGVFVIPVVTHPFAYILAIAVGALVG
                     MMLLALLKKPLNQEV"
BASE COUNT         1229 a          351 c          638 g          977 t
ORIGIN      
        1 gatgggtagt attgtatata cacctgtaaa tttggataat ataaaactaa ataagataaa
       61 aacgataaat ttctctttta attaagagaa attttttgac tttcaaggat attaatataa
      121 agttaagata taaacataaa gcaaataaat aaaaaagagg tggcaatatg atttatacag
      181 tgacattaaa tccatcaata gattatatag ttaagctaga tgagctaaag acaggaagta
      241 caaatcgtgt aaaagaagaa tacgtttatc caggaggaaa aggtataaat gtatctcgca
      301 tacttaagga acttggaaat gataatacat ctttaggttt tataagtggt tttactggag
      361 aatatataat aagaacattg gaagaaaaag aattgaagac agattttata aagataaaaa
      421 gtggtttttc aagaataaat gtaaaaataa aggcgttaaa ggaaactgag attaatggac
      481 aaggtccaaa tatagatgat gaagacattg atactttata taaaaaactt gataagctaa
      541 atcaagatga tattttaata ttggcaggaa gtataccatc tactttagat gaaaaactat
      601 acgaaaatat aatggctaga ttagaaaaaa agaatataaa agttgtagta gatgcaacta
      661 aaaatcttct tcttaatgtt ttaaaataca aaccttttct aattaagcct aataacgacg
      721 aattagaaga actatttggt gttaaattaa attcaattga agacatggta aaatatgcta
      781 aaagattaaa ggaaatggga gctataaatg tcttggtatc aatgggaaaa gatggagcat
      841 tgttgattac agaggaagat gaagtgctta taagtgatgt ccctaaagga aaagtaaaaa
      901 attctgtagg agcaggtgat tcaatggtag caggatttat atctgggtat ttgaatactg
      961 ggaaatatga ttatgctctt aaattgggag cagcttcagg tagtgcaact gctttttcat
     1021 atgacttagc caaaagagaa tacattgata aattggtaaa tgagatatct gtaaagcagt
     1081 tttaaaatat taaaaaatat atagaaaggt aattaatatt tatgaaaatt atagatttat
     1141 tagatgaaaa gtcaattaag ttaaatttaa attcaaaaac caaatcagaa gcaatagaag
     1201 aattagtaga tttggtcgca aatagtggaa atttaaatga taaagaaaac tacaaaaaag
     1261 caattttggc tcgtgaagaa atgagtacaa caggaattgg agaaggtgta gctatacctc
     1321 atgctaaaaa ttcttcagtt actaaagctt gtatagcagc agcagtgtca aaagaaggta
     1381 tagattatga atcgtttgac ggaagtttat caaatctatt ttttatgata gctgcaccag
     1441 acggagctaa caatactcat cttgaagtat tatcaagatt atctacaata cttatggatg
     1501 aagactttag aaataaactt ataaattcaa gctctgaaaa agaatttaag gaaataatag
     1561 ataaaaagga aagagaaaaa ttcagtgaag aatatcaaga tgagaaagtg gctgaaaaaa
     1621 atataataga tactaaagag aatgatgcta ataagtatcc taaggtttta gcagtaacag
     1681 catgtccaac tggaatagca catacattta tggcagctga aagtttaaat aaaatggcag
     1741 aaaacaaagg tgtatcaata aaagtagaaa ctaatggttc agcaggtgtt aaaaataagc
     1801 ttacaaaaga tgaaatagaa aatgcaacat gtataatagt ggcagcagat aaaaatgttg
     1861 agatggctag atttaatggc aaaaaagtca taaaaactaa ggtggctgat ggtatacaca
     1921 aagcggaaga acttatagac aaggctgtaa atggagatgc tccaatttac catggaggag
     1981 atggaagtca caacgaatca aatgaagaaa gtgaaagtgg atttagaaaa gtatacaaac
     2041 acttaatgaa tggtgtatct aatatgttac catttgttat tggtggaggg atacttatag
     2101 cgatagcctt tttacttgat gattatacta ttaacccttc aaactttggt tcaaatacac
     2161 cgatagcagc gttttttaaa gggataggag ataaagcttt tggatttatg ttacctgtac
     2221 ttgctggata tatagcatac tctatatctg atagaccagc gtttgttgta ggatttgtag
     2281 gaggtgctct agcaggtgat ggtggttcag gatttttagg tgcactactt gcaggtttta
     2341 tagcaggtta tttagtagaa gggcttaaga aaatatttag tgtactacca gcttcattgg
     2401 aaggaataaa accagtattg ttataccctt tacttggaac acttttgatg ggaattataa
     2461 tgacattttt agtaatacct ccagttacag caattaataa tgcaatggtt ggatttttaa
     2521 atggtttagg cggaacttca aaaatagttt taggattagt attaggtgga atgatggctg
     2581 tggatatggg tggacctgta aataaagcag catatgtatt tggagttgca tcacttgaat
     2641 cagggcagtt tgaaataatg gcagcagtaa tggctggtgg tatggttccg ccacttgcta
     2701 tagctcttgc aactacattt tttaagaata gatttactaa agaggaaaga gattctggta
     2761 aagttaatta tgtaatggga ttatcatttg ttactgaagg agctatacct tttgcagctg
     2821 gagacccact tcatgttata ccagcttgtg ttggaggttc agcagtggca ggagcactta
     2881 gtatgttgtt caacgcagct cttcgtgcac ctcatggagg agtatttgtt ataccagtgg
     2941 ttacccatcc atttgcatat atattagcta tagctgtagg tgctttggtt ggaatgatgc
     3001 ttttagcatt acttaaaaaa ccattaaatc aagaagtata gcaattaaag gatattcaag
     3061 aacttaaaat atgatgtttt ataaaaatta atatattgct gaattatttt aaaataagga
     3121 ttttattaaa ataaggattt tattaaaata aggattttat gaggctgtta tataacgatt
     3181 tgtaattgtt atctt
//
LOCUS       DAMJWP010000293         3158 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.300, whole genome shotgun sequence.
ACCESSION   DAMJWP010000293 DAMJWP010000000
VERSION     DAMJWP010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.300"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(259..516)
                     /locus_tag="PC700_003425"
     CDS             complement(259..516)
                     /locus_tag="PC700_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2582690.1"
                     /translation="MKIKVEDIPVQFHAMVEIIGIDKFIEVAKLYGGTNTYIPTYKGL
                     FRYARNRDIVKQFNGVNHNELAIRYNMCVSNIKRILNENNV"
     gene            1096..1317
                     /locus_tag="PC700_003426"
     CDS             1096..1317
                     /locus_tag="PC700_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HDF2582691.1"
                     /translation="MEVVSTKNLSSIKLKLDAGFDDKGKAVVKSKSFANVKAEALNED
                     VYAVAEAIASLQENPIVDILKLDSTSLSK"
     gene            2009..2239
                     /locus_tag="PC700_003427"
     CDS             2009..2239
                     /locus_tag="PC700_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HDF2582692.1"
                     /translation="MEIKKKLVMSFKNVKDKQVSFSIENPKDDLTEENIKSVMDLIVS
                     KNIFSVGGFDLASVVEAKVVETNTTPYDLVIE"
     gene            2252..2401
                     /locus_tag="PC700_003428"
     CDS             2252..2401
                     /locus_tag="PC700_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HDF2582693.1"
                     /translation="MDDSLQLLITNVGFPIALSMYLLVRIEGKLNTLSNSIDTLSKNI
                     LIFKK"
     gene            2658..3095
                     /locus_tag="PC700_003429"
     CDS             2658..3095
                     /locus_tag="PC700_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HDF2582694.1"
                     /translation="MKIHVEQGINCDDIEITIKCSALDNNIEKIISLLSTPLLDIKGK
                     KSGEIYKLNVCDILYFETVGNKTFAYYEDDVFEIDLKIYKLSKLLANTSFSRISKSMI
                     LNMDYIKSIKVISHNRMQATLKNNEKVIINRRYIKNIKEKLNI"
BASE COUNT         1272 a          401 c          337 g         1148 t
ORIGIN      
        1 tatttatata gtttatttat ttatttttcc cctttataca agcaattgta tcattttatc
       61 tattttttag ccaacaaata ttttcgacca ttttctttgt attctcaata tatctccagt
      121 atttaacaaa tttttttata gaatatacaa tgtatttaaa ttaataagta gtaaatttag
      181 ttaatttgtc taaaaaaact ttataaaaca tcttctttgt ctttactctt acatttaaca
      241 aggctatttt cattatgttt aaacattatt ttcattgagt attcgcttaa tattgcttac
      301 acacatatta tatctaatag ccaattcatt atggttgact ccattaaatt gttttactat
      361 atccctattt ctagcatatc taaaaagccc cttatacgta ggtatataag tatttgttcc
      421 tccatacaac ttggcaacct ctatgaactt atcaattccg attatttcta ccattgcatg
      481 aaattgaact ggtatatcct ccacttttat cttcataagt tacacctcct ttaagatatt
      541 tttatagact taacaaagtt tttatttaat acattattga ctaaactata tttattagtt
      601 ttattgaatc taataaaaaa tttcttagct ttaaaattaa tttttgtatt aactttaata
      661 aaatttctca taacttataa ctttgttagc tttaataaag attttaatgg tttaaaagta
      721 aaatgttaat attggacttt taactttttt atttacagat taaatttaat aaaattttta
      781 acataagctt taacatttaa tttaacataa actttggtac ctaacattta actctacaca
      841 tatgaaaatc catacaaaac tatctcaact aagcatgaat atatacaaat actatttcaa
      901 aacactaaga acatatcttc aaaaataact tgtaaacttt ttcatcatca gaaaagtaaa
      961 atttttccta taaaaattta cacaaaaaat ctttagaaat tactgtttaa ctccgatatt
     1021 atcttagatg atagtattat agctaacaaa gaaagtcgac aaatctttga aattaaattt
     1081 ttaggaggta attttatgga agttgtttca acaaaaaatt tatctagtat aaaactaaaa
     1141 ttggatgctg gttttgatga taagggtaag gcagttgtta aaagtaaatc atttgcaaat
     1201 gtaaaagcag aggcattaaa tgaagatgtt tatgctgttg cagaagcaat agcaagtctt
     1261 caagagaacc ctattgtaga tattttaaaa ttagatagta catcattatc taaatagtat
     1321 cttttaaacg tactctgctt tacaaattaa aaaaattaac tctttatata aaagattaag
     1381 ttatataacg cacttaatac gctatagatt tggcagatta taacttcaat tttggttata
     1441 actttaatat attaatgctt tgaaaaatta atatattaaa cgtatttcaa cttaaacatg
     1501 ttagctattt taaatgctac cctactttaa tctaattaat tgaaataaat taaatatttt
     1561 ctatatcagc aataactaga gtcaacaatt aaaatactaa tattgacaat tgaaacactg
     1621 atactaacaa acaaaacact aatattgaca attgaaacac tgatactaac aaacaaaaca
     1681 ctaatattga caattgaaat actaatacta acaagcaaaa cactaatatt gacaattgaa
     1741 atactaatac taacaagcaa aacactaata ttgacaattg aaatattaat actaacaagc
     1801 aaaacactaa tattgacaat tgaaatatta atactaacaa gcaaaacact aatattgaca
     1861 attgaaatac taatactaac aagtaaaaca ttaatattga caattgaaat attaatacta
     1921 acaagcaaaa cactaatatt gacaattaaa ataccaatat taacaagcaa acaattaaaa
     1981 tataaattat tatatcggag gtaattatat ggaaattaag aaaaaattag tcatgagctt
     2041 taaaaatgta aaggataaac aagtttcttt ttctatagaa aatccaaagg atgatttaac
     2101 tgaagaaaat ataaaatctg tcatggattt gatagtatct aaaaatattt tttcggttgg
     2161 aggatttgac ctagcttctg ttgttgaagc caaggttgtg gaaacaaata ccactcctta
     2221 tgacttagtt atagaatagg gggctaattt tatggatgat agtttacaac tgttaataac
     2281 caatgttggt tttcctatag ctctatccat gtacttgttg gttagaattg agggtaaatt
     2341 aaatacttta tcaaacagta ttgatacatt atccaaaaat attttgatat ttaagaagta
     2401 atttgacaat atatgtaagt cataaaatag gtctatttgt aaaataatta gacatattct
     2461 atgctaaata ctaggatatt tggataattt atccaaatat ccatagtatt tttttgaatt
     2521 ttaccacctc aaatttgaat ttcactattt tatttttgaa tttcactatg cttaatttga
     2581 aatttagaac gcatctagtt ggactcaata agatataatg ctattctaat gacagtaata
     2641 aaatgaggtg atgattaatg aaaatacatg tcgaacaagg tattaattgt gatgatattg
     2701 aaataaccat aaaatgttct gctctagata ataacattga aaaaataatt tcattattga
     2761 gtacaccatt acttgatata aaaggtaaaa aatcaggaga gatttataaa ctcaatgtct
     2821 gtgatatttt atactttgaa acagttggaa ataaaacatt tgcttattat gaagatgatg
     2881 tatttgaaat tgatttaaaa atatataaat tatcaaaatt attagctaat acatccttct
     2941 cacgaatatc taaatccatg attttaaata tggactacat aaaaagtata aaagtaatat
     3001 cacataatcg catgcaagct actttaaaaa acaacgaaaa agttattata aatcgtcgtt
     3061 atataaaaaa tattaaagaa aagctaaaca tttaataaaa atctaaagta atttattaat
     3121 atcactaatt gcaaatttat tgacctattt aataatta
//
LOCUS       DAMJWP010000294         3148 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.301, whole genome shotgun sequence.
ACCESSION   DAMJWP010000294 DAMJWP010000000
VERSION     DAMJWP010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.301"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            89..982
                     /locus_tag="PC700_003430"
     CDS             89..982
                     /locus_tag="PC700_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2582695.1"
                     /translation="MQLYNTPFNTNKHLIYFFTSTLVLNWIFSLTPALLGITNTILGE
                     IIFYLAYTSPAIVGLFFISTIYPQSAKQDFIYRYLSFKEIRIKWILLTILFFALTFIT
                     SLIIGNYFNVKTSDIDWIHIAVFRPSKIAYMLLASFILSILSQEAGWRGYAIDKLLVR
                     FGFIGSSIILGLVCGIWYLGSYFTPNQIPYNLAQYSLFDAFLFIPNIVLLNFIINFVY
                     INTSRSILSAGLVHMMYYFFNIQLLLHYPIKLCVIAQYVQIIFGLIFLVYMISSNKFK
                     QKVNSEIEQIKSDDFEFELDW"
     gene            1160..1984
                     /locus_tag="PC700_003431"
     CDS             1160..1984
                     /locus_tag="PC700_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2582696.1"
                     /translation="MFKIGEVSKLTQISIRMLRYYDELGILKPAEIDKYTGHRLYSVE
                     QISILQRIVLLRDSKFSVAEIANIVHNWNDEFVIKELNRKKNEIQKEIKHEQQRINKI
                     DKFIEAINCDKDEIHYNVVFKKIPSYKIISLREVVPDYQSEGILWEKLSKFIKEEHIE
                     VSRQPNNNIAFYHDEEVKDNGVDIEVGMVVKKIGKNKSGFIYRETEEIDMMACTMVYG
                     PYENIAGAYESFCYWLDKNSDYQISGINRQIGHKGEHNEINPENYLTEIQIPLIKV"
     gene            2083..2691
                     /locus_tag="PC700_003432"
     CDS             2083..2691
                     /locus_tag="PC700_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2582697.1"
                     /translation="MEKLLRGKIMKADSTKEQLINATIQLLSSHRDVSSITAREIVAK
                     ANVNLAMINYYFKSKEELINISIKKFLKRHMDEYKATHDTKNLAPKQQLREILIAFFD
                     FIGEHSQFIKISVPYILIQEEIVYPFEILPLIKAHYENQNNKKTEAEYRTIAYQITSF
                     IQLLFLRSNAFFKYSGIDILNVEQRNGLIDFQLDLLLVDITK"
     gene            2795..2971
                     /locus_tag="PC700_003433"
     CDS             2795..2971
                     /locus_tag="PC700_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582698.1"
                     /translation="MFFTFIGVISAILLLCTIICGLWIKLNNRNNDSNSHFTFSILSI
                     AFSLLIVVAFLVRN"
BASE COUNT         1177 a          378 c          447 g         1146 t
ORIGIN      
        1 catcaaacga taacttaata catataaaaa taaataaaca attaataaaa attaaaactt
       61 cattataaaa cggaaggata gtgaaaaaat gcaactatat aacaccccct ttaatacaaa
      121 caaacactta atctatttct ttacatctac gctagtattg aactggatat tcagtcttac
      181 acctgcgttg ctcggaatta caaatacaat acttggagaa atcatttttt atcttgcata
      241 tacatcacca gctattgtag gtctattttt tatttctaca atctatccac aaagtgcaaa
      301 acaagatttt atttatagat acctaagctt taaagaaatt agaataaaat ggatactatt
      361 aacaatatta ttttttgctt taacttttat tacttcgtta ataatcggaa attactttaa
      421 tgtaaaaact tctgatatag attggataca catagcagtg tttagacctt ctaaaatagc
      481 ttatatgcta ttagcaagtt ttatattgag tatacttagt caagaagctg gatggagagg
      541 atacgccatt gacaaattat tagttagatt tggatttata ggctcttcaa ttattttagg
      601 gcttgtttgt ggaatctggt atctagggtc gtattttaca cctaatcaaa taccatataa
      661 tttggcacaa tactccctat ttgatgcttt tttatttata ccaaacattg tacttttaaa
      721 ctttataatt aattttgtat atatcaatac aagtagaagc attttatcag ctgggttagt
      781 tcatatgatg tactattttt tcaacattca attacttcta cactacccta ttaaattatg
      841 tgtaatagct caatatgtac aaataatatt tggtttaata tttcttgtat atatgatttc
      901 ttctaataaa tttaaacaga aggtaaattc tgaaattgaa cagataaagt cggatgattt
      961 tgaatttgag ttagactggt aattttaata tgaattataa attaagataa tctatgattt
     1021 gatacgatta ctacttaaat ataatttact ctctataatt tattgcaaca tctggaaaat
     1081 aattgtattt ccacaatcgc ttgaagtata taaaaacaaa atatatacta attataagtg
     1141 aaaaggaggt catattttca tgtttaaaat aggagaggtt tcaaaactaa cacaaatttc
     1201 tattcgtatg ttgcgttatt atgatgaact tggtatttta aagcctgcag aaatagacaa
     1261 atatacaggt catagattat actcagttga acagatttca atattacaaa gaattgtatt
     1321 attgagagat tcaaaattta gcgttgcaga aatagcaaat atcgtacata attggaatga
     1381 tgaatttgta attaaagaat tgaatagaaa aaagaatgaa atacaaaagg aaataaagca
     1441 cgaacaacaa agaataaata aaattgataa atttatagaa gctataaact gtgataaaga
     1501 tgaaattcat tacaatgttg tctttaaaaa gattccaagt tataaaatca tatcacttag
     1561 agaggtagtc ccagactacc aatctgaagg tatactctgg gaaaagttat ctaaatttat
     1621 taaagaagaa catatagaag tatcacgtca acccaacaat aatatagctt tttaccatga
     1681 tgaagaagtc aaagataatg gtgtagatat tgaagttgga atggttgtaa agaagattgg
     1741 aaagaacaaa tctggattta tatatcgtga gacagaagaa attgatatga tggcatgtac
     1801 tatggtttat ggtccatatg aaaatatagc tggtgcatat gaatcgttct gttattggtt
     1861 agataaaaat agtgattatc aaatatcagg tataaataga caaatcggtc ataagggtga
     1921 gcataatgaa ataaatcctg aaaattatct tacagaaatt caaatccccc ttataaaagt
     1981 ctaatcgatt agtacttaaa tacttaagta ctaatcaccc attgacatcc caaaattttc
     2041 atgatagtat ctaaacgtgt attaaacatg tttaattttt ttatggagaa acttttaagg
     2101 ggaaaaatta tgaaagcaga ttcaacaaag gaacaattaa ttaatgccac aatccagtta
     2161 ctatcttcac atagagatgt ttcaagcatt acggctagag aaattgtagc taaagcaaat
     2221 gtcaatttag ctatgattaa ttattatttt aaatctaaag aggagttaat taatatttct
     2281 attaaaaaat ttttaaagcg tcatatggat gaatataagg ctacacatga tactaaaaat
     2341 ttagcaccta aacagcaatt aagggaaata ctaattgcat tttttgattt tataggtgaa
     2401 catagccaat ttataaagat ttctgtaccc tatatattga tacaagaaga aattgtttat
     2461 ccatttgaga tactaccttt aattaaggct cactatgaaa atcagaataa caaaaaaact
     2521 gaagctgaat acagaactat tgcgtatcaa ataacttcat ttattcagtt gctttttctt
     2581 cgttctaatg cttttttcaa gtatagtggt attgatattc ttaatgttga acagagaaat
     2641 ggtttaatag attttcaatt agatttatta ttggtagata ttacaaaata atactatatt
     2701 aattttttat gtacattttt ttattttatg gtaacgtttg aaaatattct cattgactaa
     2761 aaatacttgc attaaataag ggggaggaat tttaatgttt tttacattta taggagtaat
     2821 atcagcaatt ttattgttgt gtacaattat atgtggttta tggataaaac ttaataatcg
     2881 taataatgac tctaattctc actttacttt ttcaatactt tcaattgctt tttcactgtt
     2941 aattgtggtg gcctttttag taagaaatta atgtctttgt tatgctgggg tttattatta
     3001 tagattttta ttattgcatg atttttacta tattatagta tttttattat attacagggt
     3061 cttatttatt atagtgtttt gttattacag cattttatta tattacagag tcctatttat
     3121 tataggattt ttattatatt gtaaaatc
//
LOCUS       DAMJWP010000295         3129 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.302, whole genome shotgun sequence.
ACCESSION   DAMJWP010000295 DAMJWP010000000
VERSION     DAMJWP010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.302"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     misc_binding    77..339
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            415..3081
                     /locus_tag="PC700_003434"
     CDS             415..3081
                     /locus_tag="PC700_003434"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDF2582699.1"
                     /translation="MENTNLSKTYNPKDFEARLYKKWMDEGYFKSKPNPDKKPFTIMM
                     PPPNITGQLHMGHALDHTLQDILIRWKRMDGYEAFWLPGTDHASIATEVKVVERIKKQ
                     EGKTKYEIGREEFLKRAWEWKDEFGGKISNQLKQLGDSCDWDKERFTMDEGCNEAVIE
                     FFVSLYEKGHIYRGNRIINWCPDCKTTLSDAEVEHEEHDGNFYHIKYPLKDSEDFLEI
                     ATTRPETMIGDTGIAVNPEDDRYKHLIGKTAILPLVGRELPIVADSYVDLEFGTGAVK
                     MTPAHDPNDFEVGLRHNLEQLNTMNEDGTMNEVCGKYEGMDRFECRKAIVADLKEQGY
                     LIKIKAHNHNVGTCYRCHTVVEPRLSEQWFVKMEELAKPAIDILKKGELEFVPDKFDK
                     TYLQWLENIRDWCISRQLWWGHQIPAYYCQECGEIVVARKMPDKCPKCGSTHFKQDED
                     ALDTWFSSALWPFSTLGWPNKTEALDYYYPTSVLVTGYDIIFFWVVRMAFAGMFCMNE
                     KPFDHVLVHGLVRDSQGRKMSKSLGNGIDPLEIIEQYGADALRFTLTTGNSPGNDMRF
                     YMERVEFARNFANKLWNASRFVFMNIDEDIIKNMTRESVKEDLTLADKWIISRANNIV
                     KEATNNMDKFDLGIALQKIYDFTWSEYCDWYIEMVKPRLYGEDANAKSAALYTLTYVL
                     EKILKLLHPYMPFITEEIYTHLPTVEGCIIVSEWPKYNEEDNMAEEEDMMNLLMEGIR
                     SIRNVRAEMNVPPSKKAKLIIIPSEEKIEAIELGKDYFITLASASNVEIAKDKSNVPE
                     DAVGVVIDGVEIFIPLNELVDFEKEIERLSKEKKKLEGEIKRVNGKLANQGFLAKAPE
                     SLIEEEKVKKEKFEEMIKSVEERLTNLESKIK"
BASE COUNT         1263 a          349 c          612 g          905 t
ORIGIN      
        1 attaagaaaa ttcagaaaaa ataaaataaa aataattgac aaagagaata atatttgtat
       61 ataataatat gtaatattaa agactgagaa gaagaggagt aagtaaatac tactaaaaga
      121 gaggaaaatt cactggctgt aagattttct aagtataaga tttactgaat gtagctttgg
      181 agtttctaaa ctgaactata agtaagttta gacggttaga accgttaaat tctttgagag
      241 tcaaagttga taaaataaca ttaactaata atttaattat atgttaatgt attttaagct
      301 ttggaattaa ggtggtaacg cgagcttttc gtccttttta aagaggatga agagctcttt
      361 tttatttcta aagatattta aaaaatttat ttataaaata caggaggcag gacaatggaa
      421 aatacgaatt tatcaaaaac ttataaccca aaagattttg aggctaggct atataaaaaa
      481 tggatggatg aagggtattt taaatctaaa ccaaatccag ataaaaaacc atttacaata
      541 atgatgccac caccaaatat aacagggcaa ttgcatatgg gacacgctct tgaccataca
      601 cttcaagata tattaatcag atggaaacgt atggatggat atgaagcttt ttggcttcca
      661 ggaactgacc atgcttctat agcaacagaa gttaaagttg ttgaaagaat aaaaaaacaa
      721 gaaggcaaga caaagtatga aataggtaga gaagaatttt taaagagagc ctgggaatgg
      781 aaagatgaat ttggaggaaa aatatctaat cagcttaaac aactaggtga ttcttgtgat
      841 tgggataaag aaagatttac tatggatgaa gggtgtaatg aagcagttat agaatttttt
      901 gtaagcctat atgaaaaagg tcacatatac agaggaaata gaataataaa ctggtgccca
      961 gattgcaaaa ctactttatc agatgcagaa gttgaacatg aagaacacga cggaaacttt
     1021 tatcatataa agtacccact aaaagatagt gaagactttt tagaaatagc tacaactaga
     1081 cctgagacaa tgataggaga tactggtata gcagtaaatc cagaagatga tagatataaa
     1141 catttaatag gaaaaacagc aatacttcca ttagtaggaa gagaacttcc aatagttgca
     1201 gatagttatg ttgatttaga gtttggaact ggtgcagtta agatgactcc agcacatgac
     1261 cctaatgatt ttgaagtagg attaagacat aatcttgagc agttaaatac tatgaatgaa
     1321 gatggaacta tgaatgaagt ttgtggaaaa tatgaaggta tggatagatt tgagtgtaga
     1381 aaagctatcg tagcagactt aaaagaacaa ggttacctaa ttaagataaa agcgcataat
     1441 cataatgtag gaacttgtta tagatgccat acagtagttg agcctagatt atcagagcaa
     1501 tggtttgtta agatggaaga acttgcaaaa ccagctatag atattcttaa aaaaggagaa
     1561 ttagaatttg ttccagataa atttgataaa acttatttac aatggctaga aaatataaga
     1621 gattggtgta tatcaagaca attatggtgg ggtcatcaaa taccagctta ttattgtcaa
     1681 gagtgtggag aaatagtagt tgctagaaaa atgccagata agtgtcctaa atgtggaagc
     1741 actcatttta aacaagatga agatgcctta gatacttggt tcagttctgc attatggcct
     1801 ttctctactc taggatggcc aaataagact gaagctttag attattatta tccaacaagt
     1861 gtacttgtta caggatacga tataatattc ttctgggtag ttagaatggc atttgcaggt
     1921 atgttctgta tgaatgaaaa gccatttgac catgttttgg ttcatggatt agtaagagat
     1981 tctcaaggta gaaaaatgag taaatcttta ggaaatggta tagacccatt ggagataatc
     2041 gagcaatatg gagctgatgc cctaagattt acattaacaa ctggaaattc cccaggaaat
     2101 gatatgagat tctatatgga aagagtagaa tttgctagaa actttgcaaa taagttgtgg
     2161 aatgcatcaa gatttgtatt tatgaatatt gatgaagata taattaaaaa catgactaga
     2221 gaaagtgtta aagaggactt aactctagct gataaatgga taatatcaag agcaaataat
     2281 attgtaaaag aagcaactaa taacatggat aagtttgatt taggaatagc acttcaaaag
     2341 atatacgatt ttacttggtc agaatactgt gactggtata ttgaaatggt taaacctaga
     2401 ttatatgggg aagatgcaaa tgctaaatct gcagcattat atacattaac ttatgttctt
     2461 gaaaaaatat taaaattatt acatccatac atgccattta taacagagga aatatatact
     2521 catcttccta ctgttgaagg atgtataata gtatctgaat ggccaaaata taatgaagaa
     2581 gataacatgg ctgaagaaga agatatgatg aacttattga tggaaggtat aagaagtata
     2641 agaaatgtga gagcagaaat gaatgtacca ccttctaaga aagctaaatt gataataatc
     2701 ccaagtgaag aaaaaataga agctattgag cttggtaaag actattttat aactctagca
     2761 tctgcttcaa atgtagagat agcaaaagat aaatctaatg ttccagaaga tgcagttgga
     2821 gtagttattg atggtgttga aatatttata cctcttaatg aattagttga ttttgaaaaa
     2881 gaaattgaga gattgtctaa agaaaagaaa aagttagaag gcgaaataaa gagagttaat
     2941 ggaaagcttg caaatcaagg tttcttagca aaagctcctg aaagtttaat tgaagaagaa
     3001 aaagtaaaaa aagaaaaatt tgaagagatg attaaatctg ttgaggaaag attaacaaat
     3061 ttagaatcta aaataaaata aatataagaa ttagtaaaaa catacaaaaa gtatatattc
     3121 tatactttt
//
LOCUS       DAMJWP010000296         3066 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.303, whole genome shotgun sequence.
ACCESSION   DAMJWP010000296 DAMJWP010000000
VERSION     DAMJWP010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.303"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(145..666)
                     /locus_tag="PC700_003435"
     CDS             complement(145..666)
                     /locus_tag="PC700_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDF2582700.1"
                     /translation="MATIDLIVLGIVKQQPKSAYDIQKAVEYRNISRWVKISTPSIYK
                     KVIQLEQKGYIESKTVKNGKMPEKAVYSLTELGETHFLDLMFDTSSKPINILLDFNAV
                     IVNLNSLTPEKQKLCLNNIEENVQELKKTIEENIIIKTEVPETAKAVLEQQLVLAQAL
                     ETWINSLKDNMGN"
     gene            794..2158
                     /locus_tag="PC700_003436"
     CDS             794..2158
                     /locus_tag="PC700_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2582701.1"
                     /translation="MKESNKKIELLGSAPISKALLSMGLPTMIGMMITALYNLVDAYF
                     VGGLGTSQMGAISVAFPLGQLVVGLGLLFGNGAASYIARLLGHGDNEKAGKVVSTSIY
                     SSIIVGTLVIIVIMIFLNPILKLLGATKSIMPYAISYASIYVVSSIFNIFNVTMTNIV
                     TSEGAAKTTMCAMLLGALLNMILDPIFIYTFGLGVRGASIATAISQIASTLVYVGYIL
                     SKKSIFSFRINECCFSKEIFSEILKIGIPTLIFQLLTSLAITLTNMEATKYGDSVIAG
                     MGAVTRITSLGSLVVFGFIKGFQPIAGYNFGSKNYDKLHIAIKTSILWTTIFCVVLGL
                     ILVLFPKSIISQFTSGDILLIENGSKALRANGFSFMLFGFYTVYSTLFLALGKAKEGG
                     FLGMCRQGVCFIPIILIIPVFYGLNGILYAQPIADLMAAIIAGFMSIRLHKELNLLKI
                     NGMH"
     gene            2347..3024
                     /locus_tag="PC700_003437"
     CDS             2347..3024
                     /locus_tag="PC700_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF2582702.1"
                     /translation="MISQNEIDILTHSLPFWDKLTDLQKELLISSANTSDYKKGNPVH
                     CGDSDCIGILIIKSGTIRTYILSDEGREVTLFRLDDGDVCILSASCILKTITFDVYVD
                     AETDCDIIQINSSVFAKLSTENIHAELFSYKLATERFSDVMWAMQQILFMSFDKRLAS
                     FLIDEIAKNGSSTINMTHEQIAKYMGSAREVVSRMLKYFAREGIVSLSRGGIKVLDKD
                     KLRSLTL"
BASE COUNT          959 a          439 c          496 g         1172 t
ORIGIN      
        1 aaaagtttaa cattctccct caattaagtg agctttattt taaatatact tttcagttta
       61 tatcatttgt ttcatttttt ctaatatctc attattaaat atgctggttt ttgtttaatg
      121 attattaagt tagcttctct aattctaatt ccccatatta tcttttaaag aatttatcca
      181 ggtttccaat gcttgtgcta gcaccaactg ttgctccaac actgcttttg ctgtctctgg
      241 tacttctgtc tttataatta tattttcctc aatagttttc tttaactctt gaacattctc
      301 ttcaatatta ttgagacata atttctgttt ttctggtgta aggctattta aatttactat
      361 aacagcatta aaatctaaaa gaatatttat tggtttgcta gatgtatcaa acattaaatc
      421 taaaaaatgt gtctcaccta attcagttaa tgaataaact gctttttctg gcatttttcc
      481 attcttcaca gttttacttt caatatatcc cttttgttct aattgaatta cttttttata
      541 aattgatggt gtacttattt taacccaacg tgatatattc ctgtattcca ctgccttttg
      601 aatatcataa gcactcttgg gttgttgctt tactattccc aatacaatta aatctattgt
      661 agccataaaa atcatctcct ttagttttct tactattgac aaatactata aattatagta
      721 ctattttcca tacttactat aatttatagt attataattt agacttaatg tcaatagaag
      781 aggagtgtta agtatgaaag aaagtaacaa aaaaattgaa ttattagggt ctgccccaat
      841 ctcaaaagca ttgttatcta tgggattacc taccatgata ggtatgatga taactgcatt
      901 atacaatttg gttgatgcat actttgtggg tggacttgga acaagtcaaa tgggagctat
      961 atccgttgca tttccattag gtcagcttgt tgtgggatta ggattattgt ttggaaatgg
     1021 agctgcttca tatattgcca gacttttagg ccatggtgat aatgaaaaag caggaaaagt
     1081 ggtaagtaca tctatttaca gtagtattat agtgggtaca ttagttatta ttgtgattat
     1141 gatttttctc aatccaattt taaaattgtt aggagctaca aaaagtatta tgccttatgc
     1201 aatatcttat gctagcattt atgtcgtgtc atctattttt aatatattca atgtcactat
     1261 gactaatatt gtcacaagtg aaggagctgc taaaacaact atgtgtgcta tgttattagg
     1321 tgctttgctt aatatgattt tagacccgat attcatttat acttttggtc tgggggtaag
     1381 gggtgcttct attgcaacag ctatctcaca gattgcttca acacttgttt atgtaggata
     1441 tattttaagc aagaaaagta tatttagttt tagaattaac gaatgctgtt tttcaaaaga
     1501 aattttttca gaaatcctaa aaattggcat acctacatta atatttcaat tattgactag
     1561 tcttgccatt acattaacta atatggaggc tacaaagtat ggtgattctg tgatagctgg
     1621 aatgggtgct gtaacacgaa taacatcttt aggtagtctt gttgtatttg gttttataaa
     1681 aggtttccaa ccaattgctg gttataattt tggttcgaag aactacgata aattacatat
     1741 agcaataaaa acttccatat tatggactac aatattttgt gtagtgcttg gtcttatatt
     1801 ggtgcttttc ccaaaatcta taatttcaca atttacaagt ggtgacattc tattaataga
     1861 aaatggtagt aaagctctta gggcaaatgg gttttcattt atgctatttg gattctatac
     1921 agtgtattct acattattcc tagcactagg aaaagcaaag gaaggtggat ttttaggtat
     1981 gtgtagacaa ggcgtctgtt ttatccctat aattttgatt attcctgtat tctatgggtt
     2041 aaatggtatc ttatatgcac aaccaatagc tgatttgatg gctgctataa ttgctggttt
     2101 tatgtctata aggttacata aggaacttaa tcttttaaaa ataaacggta tgcattagtt
     2161 atacaaacct actatcatca aaacatatta ctttctcatt aatagcaaga atatatatta
     2221 tcatattttt tctacataaa tcatactatt actatctgac taaataatcc tcttaacaaa
     2281 ctacttgtca atttatctat tcacttgtat catgggatta taacaattta agaaaggaga
     2341 tattgtatga tttcacaaaa tgaaattgat atacttacac atagcttgcc tttttgggat
     2401 aaacttactg acttacaaaa agaattatta atttcttcag caaatacctc ggattacaaa
     2461 aaagggaatc cagtacattg tggtgatagt gactgtattg gtattttgat tataaagtct
     2521 ggaacaatac gtacatacat actttcagat gaaggtcgag aggttacact gttccgactt
     2581 gatgatggag atgtatgtat tttatcagct tcctgtattc taaaaacaat aacttttgat
     2641 gtctatgtag atgctgaaac ggactgcgat attatacaga ttaattcctc tgtttttgca
     2701 aaattatcta cagaaaatat tcatgcagag ttattttcat ataaattagc taccgaacgt
     2761 ttttctgatg ttatgtgggc tatgcaacaa attcttttta tgagttttga taagcgtttg
     2821 gcatcttttt taattgatga gatagctaaa aatggcagta gcactataaa tatgactcat
     2881 gagcaaattg ctaagtatat gggtagtgcc agagaagttg tatcgagaat gttgaaatat
     2941 tttgctagag aaggtattgt gtctttatct cgtggtggta taaaagtatt ggataaggat
     3001 aaacttcgtt ctttgacact gtaaaagtgt gtctcaaaat gaacttttta gttcatgagg
     3061 cactct
//
LOCUS       DAMJWP010000297         3064 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.304, whole genome shotgun sequence.
ACCESSION   DAMJWP010000297 DAMJWP010000000
VERSION     DAMJWP010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.304"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            202..2844
                     /gene="adhE"
                     /locus_tag="PC700_003438"
                     /gene_synonym="adhC"
     CDS             202..2844
                     /gene="adhE"
                     /locus_tag="PC700_003438"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDF2582703.1"
                     /translation="MEKKEKVVEKSNVEVCSPEIVNSVETLRMRLEEIRLAQKEFATF
                     TQEQVDKIFLAASTAANQQRIPLAKMAVEETGMGIVEDKVIKNHFASEYIYNAYKDTK
                     TCGVIEKDEAFGFTRIAEPVGVLSAVIPTTNPTSTAIFKSLIALKTRNGIIFSPHPRA
                     KNCTIEAARVVHDAAVKAGAPKGLIGWVDVPSIELTNVVMAEADLILATGGPGMVKSA
                     YSSGKPAVGVGPGNVPAIIDESADIKMAVSSILVSKSFDNGMICASEQAVIVPEKIYE
                     EVKKEFKYRGAHFLNKEETEKVGKVVIIDGSLNARIVGQPAHVIAKMADVEVPKTARI
                     IIGEVESVELNEPFAHEKLSPVLAMYKSKSFEDAVAKAEKLVADGGYGHTSSLYADSI
                     NHPDRVEKFVNAMKTCRVLVNTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVSENVG
                     VKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVAAREFKDVMDKKKAFIVTDSFLY
                     NNGYTKKLTDLLDEMGIKHTTFFDVAPDPTLACAREGAKAMADFQPDLIIAVGGGSAM
                     DAGKIMWVMYEHPEVDFQDLAMRFMDIRKRVYVFPKMGEKAYFAAIPTSAGTGSEVTP
                     FAVITDQDSGVKYPLADYELMPNMAIIDADMMMEMPPRLTAASGVDALTHALEAYVSM
                     LRTEPADGLALQAGKIIFEYLPRAYKNGKNDKEAREKMAMASTMAGMSFANAFLGICH
                     SLAHKLGAFHHVQHGVANALLINEVIKFNCAEAPNKMGAFSQYRYPDCIQRYAEFASF
                     AGIKGNTDQEKVDNLIKAIDELKARVGLPKTIKEAGVEESKFLETLDVMVEQAFDDQC
                     TGANPRYPLMSELKEIYLKVYYGK"
BASE COUNT         1153 a          403 c          612 g          896 t
ORIGIN      
        1 aaaaataata tatttgttaa aaaataaatt gacaaaatat tcagaaaaaa acttcttgac
       61 ctaagagagt aaaataaata aaatctaagg tatagagatg aaatctataa aattatttta
      121 agtataagtt aaaaatatag atggtgcatt taagatttat aaaaattaga gatgcataat
      181 tattttaggg aggtttttag tatggagaaa aaagaaaagg tagtagaaaa atcaaatgtt
      241 gaagtatgta gtcctgaaat tgtcaacagt gttgaaacat taagaatgag attagaagaa
      301 attagattgg cacaaaaaga atttgcaaca tttacgcaag aacaggtaga taagattttc
      361 ttagctgctt caactgcagc taatcaacaa agaatacctc tagcaaaaat ggcagtggaa
      421 gagactggta tgggtattgt tgaagataag gttataaaaa accattttgc ctcagaatat
      481 atctataatg cttataaaga tactaaaact tgtggtgtca tagaaaaaga tgaagcattt
      541 ggttttacaa gaattgctga gccagtaggt gttttatcag cagttatacc aacaactaat
      601 cctacatcaa ctgcaatatt caaatcccta atagcactaa aaactagaaa tggtataatt
      661 ttctctcctc atccaagagc taaaaactgt actatagaag cagctagagt tgtacatgat
      721 gcggcagtta aggcaggagc accaaaagga cttataggtt gggtagatgt tccttcgatt
      781 gaattaacta atgttgtcat ggcagaggca gaccttatat tagctactgg tggacctgga
      841 atggttaaat ctgcatactc ttcaggtaaa ccagctgttg gagttgggcc tggaaatgta
      901 cctgcaataa tagatgagag tgcagatata aaaatggctg taagttcaat cttagtatca
      961 aaatcattcg ataatggtat gatatgtgct tccgagcaag ctgtaatagt accagaaaaa
     1021 atatatgaag aagttaaaaa agaatttaaa taccgtggag cacatttcct aaataaagaa
     1081 gaaactgaaa aagtaggaaa agtagtaata attgatggtt cattaaatgc cagaatagtt
     1141 ggacaaccag cacatgtaat agccaaaatg gcggatgtag aagttccaaa aacagctaga
     1201 ataataattg gtgaagtaga atctgttgaa ttaaatgaac cttttgcaca tgaaaaactt
     1261 tctcctgtac ttgcaatgta caaatctaaa agttttgaag atgcagttgc taaagctgaa
     1321 aaattagttg cagatggtgg atatggccat acatcttcgt tatatgcaga ctctataaat
     1381 catccagata gagtagagaa gtttgtaaat gctatgaaaa cttgtagagt attagttaat
     1441 actccatcgt cacaaggtgg tataggtgac ttatataact ttaaattagc accatctctt
     1501 actcttggtt gtggttcttg gggtggaaac tcagtatctg aaaacgtggg agtgaaacat
     1561 ttacttaata tcaagacagt agctgaaagg agagagaata tgctttggtt tagagcacca
     1621 gaaaaggttt atttcaaaaa aggttgttta ggtgtagcag caagagaatt taaagatgtt
     1681 atggataaaa agaaagcctt tatagtaaca gattctttcc tttacaataa tggatacact
     1741 aagaagctaa ctgatttgtt ggatgagatg ggaattaagc atactacatt ctttgatgtt
     1801 gctccagacc caacattagc ttgtgcaaga gaaggagcta aggctatggc tgatttccaa
     1861 ccagatttaa taatagctgt aggtggaggt agcgcaatgg atgctggtaa gataatgtgg
     1921 gtaatgtatg agcatccaga ggtagacttc caagatttgg ctatgagatt tatggatata
     1981 agaaaaagag tatatgtatt tcctaagatg ggtgaaaaag cgtattttgc tgctatccca
     2041 acttcagcag gaactggttc agaagtaact ccatttgcag ttataactga ccaagattca
     2101 ggtgtaaagt acccattagc agattatgaa ttaatgccaa acatggctat aattgatgct
     2161 gatatgatga tggagatgcc accaagatta acagcagcat caggtgttga tgctttaaca
     2221 catgcattag aagcatatgt atctatgtta agaactgaac cagctgacgg tctagcacta
     2281 caagcaggta aaataatatt tgaatatcta cctcgtgcat acaaaaatgg aaagaatgat
     2341 aaagaagcaa gagaaaaaat ggcaatggca tctactatgg caggaatgtc ttttgcaaat
     2401 gccttcttag gaatatgtca ctctttagca cataaattag gtgcattcca ccatgttcaa
     2461 cacggtgttg caaatgcatt attaataaat gaagtaatta agttcaattg tgcagaagct
     2521 ccaaataaga tgggtgcatt ctcacaatac agatatcctg attgtataca aagatatgct
     2581 gaatttgctt cttttgcagg aatcaaggga aatacagacc aagaaaaagt agataacctt
     2641 ataaaagcaa tagatgaatt aaaggcaaga gtaggacttc ctaaaactat aaaagaggct
     2701 ggagtagaag aatctaaatt cttagaaaca ttggatgtta tggttgaaca agcttttgat
     2761 gaccaatgta caggtgctaa tcctagatac ccattgatga gtgaattaaa agagatatat
     2821 ctaaaagttt attatggtaa ataatattca ttgtacaaca taggttctat tgggtttgta
     2881 tataaattat tagatttgaa tataaataga gttttattag agaaacgtag aagtaactat
     2941 aaataataga ttataactta aattctgaat ggaaatgata aaaagtcagt aatttaatgg
     3001 taatttcgaa tatgtgtatt taatcaatta actttaaaat tagtcggaaa atttttttat
     3061 taca
//
LOCUS       DAMJWP010000298         3063 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.305, whole genome shotgun sequence.
ACCESSION   DAMJWP010000298 DAMJWP010000000
VERSION     DAMJWP010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.305"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(6..398)
                     /locus_tag="PC700_003439"
     CDS             complement(6..398)
                     /locus_tag="PC700_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2582704.1"
                     /translation="MAALKSFEKPLTPEEEIEYLTKFKIENDKSAKDTLIERNMRLVA
                     YIAKKYNNSTEDQDDLISIGTIGLIKAIETYNIDKGTRLATYASRCIENEILMNIRSN
                     KKNKTQVSLQDPIGTDKEGNEINTTLYM"
     gene            966..1328
                     /locus_tag="PC700_003440"
     CDS             966..1328
                     /locus_tag="PC700_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582705.1"
                     /translation="MKIRKSGFISIECIISIAILYVAVYLVSTSLYNCYSFISRNISD
                     REMLSTAKKYIEDEKYRIQNSKYELIEDKIEKNYINGYEINSRIEQILDYYQCYEINI
                     EIKNEFKKLRFNSYVTRK"
     gene            1312..2028
                     /locus_tag="PC700_003441"
     CDS             1312..2028
                     /locus_tag="PC700_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582706.1"
                     /translation="MLLENKGSILIITLIIFSIISTVCIMCIGLIYTHNNMFELGYKD
                     IQLKEISLSGIEISVSNVLSCVEEAIDVSNNEDEFREYFLGNNMIECIKKIKDTSYSS
                     LEELSLKVENNTIYDRGDFFEFDIESNVREKEFLKAIKVRVKIKNPWIGIDIEDYMDK
                     SNNNKIDDKTFNNYKASENNEILNQFKNESENIRNQEVVEFTEEDEEYEEELKQNIIE
                     NIKKNFDEKKLVQVYKYRGV"
     gene            2048..2467
                     /locus_tag="PC700_003442"
     CDS             2048..2467
                     /locus_tag="PC700_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582707.1"
                     /translation="MTVIELIVSISIILLIVTLSFPKDNLENHKINLFARQLCSDIRY
                     VRRINMFSNYNTYIYYINQGKHEGYVLRKDGKNIKSIMLPKNAKIARSIEIIKFKSDG
                     SPQKASTIEIYNNRLRKTITITPVSGRVLLKEGKYET"
     gene            2457..2981
                     /locus_tag="PC700_003443"
     CDS             2457..2981
                     /locus_tag="PC700_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582708.1"
                     /translation="MKHKYGYLLLESVVSLSSMVIIILVLYSIFLSTINLKLKVEDKI
                     ELQQQSLEIIKSMEGIISNSMGIMNVSNYEETFKKTTSIKCRYVDENNNEESISNKEI
                     ILNERRNKLFVNSLNGESSQAGGYEIGDYVDEMYVLITNNGQYVNIKLKLSKRSQKYE
                     TDFKIKVWNFSESI"
BASE COUNT         1284 a          255 c          481 g         1043 t
ORIGIN      
        1 gaaaattaca tataaagagt agtatttatt tcattacctt ctttatctgt tccgatgggg
       61 tcttgtagag atacttgagt tttatttttt ttgttagatc taatattcat taaaatctca
      121 ttttcaatac atctagaagc atatgttgct aatcgagttc ccttatctat gttataagtt
      181 tctatagctt taattaatcc tattgtacct atggatatta aatcatcctg atcttccgtt
      241 gaattattat atttctttgc tatataagct actaatctca tgtttctttc aattaatgta
      301 tcttttgcac ttttatcgtt ttctatttta aatttagtta aatactcaat ctcttcttca
      361 ggggttaagg gtttttcaaa agatttaaga gctgccaata ggtcaccccc ttttcaacgg
      421 tcactttaaa ttatatgaag gagacttatc caagttgact gttatctaat aaaataatat
      481 gcatattttt agaatttaaa acatatattt gcaaactttt gaatggaata tgacataaag
      541 agtgggaatt taaatttaag tttttgttgt atatatatga aacaaggcga agaaattatt
      601 gatttataaa gtataaattg gaattgaggt gtcaataaat ataaaaggaa atagtattta
      661 tatatgtttt ggtgttgagg taaaagatta atgcaaagca ttagagtcta taaaaatatg
      721 aatataggag gtgtaatttt acaatactta aaaaagtaaa tgtaattaat tgtttgtata
      781 aagttgatta caagcaaaaa gtatcagtca tagttaattc tgttaaggat gaaggtggtg
      841 tgttaattta tatatgtatg ggaagaaatt ttgattacaa tgaaaaaact tacaaaagat
      901 atttatgaaa aaacttatag ttaaatgttt atatataaaa tattatttag gggttgtttt
      961 tattattgaa aataagaaag agtggtttta tatcaatcga atgtataata agtattgcta
     1021 tattatatgt ggctgtttat ttagtttcta catcattgta taattgttat agttttatca
     1081 gtagaaatat atctgacaga gaaatgttaa gtacagcaaa aaaatatata gaagatgaga
     1141 agtatagaat acaaaatagt aagtatgagt taattgaaga taagatagaa aaaaattaca
     1201 taaatggata tgaaattaac agtagaatag agcaaatttt agattattat caatgctatg
     1261 aaataaatat agagataaaa aatgaattta aaaaactgag gtttaatagc tatgttacta
     1321 gaaaataaag gctctatatt aataataaca ttgataatat tttctattat aagtactgtg
     1381 tgcattatgt gtataggttt aatttataca cataataata tgtttgagtt aggatacaag
     1441 gacattcaac taaaagaaat ttctttaagt ggaatagaaa tatcagtaag taatgtactt
     1501 tcttgtgttg aggaagctat tgatgtatct aataatgaag acgaatttag agaatatttc
     1561 ttaggaaata atatgattga atgtataaaa aaaataaaag atacatctta tagtagttta
     1621 gaagaacttt ctttaaaagt agaaaataat actatttatg acagaggaga tttttttgaa
     1681 tttgatatag aaagtaatgt tagagaaaaa gaatttttaa aagcaataaa agtaagagtg
     1741 aaaatcaaaa atccatggat aggaattgac attgaagatt atatggataa atctaataac
     1801 aataaaattg atgacaaaac attcaataat tataaagcat ctgaaaataa tgaaattcta
     1861 aatcaattta aaaatgaaag tgaaaatata aggaatcaag aggttgttga atttacagaa
     1921 gaagatgaag aatatgaaga agaacttaaa caaaacataa ttgaaaatat aaagaagaat
     1981 tttgatgaaa aaaaactagt acaggtatat aagtataggg gtgtataact ttgaaaaaag
     2041 aaggaatgtg actgtaatag aattaatagt atctatcagt ataatactct tgatagttac
     2101 attatctttt ccaaaagata atcttgaaaa tcataaaata aatttatttg cacgtcaact
     2161 ttgttcagat ataaggtatg ttaggcgaat taatatgttt agcaactaca atacatatat
     2221 atattatata aatcaaggta aacacgaagg ttatgtattg agaaaagatg gtaaaaacat
     2281 taaatcaata atgctaccta aaaatgcaaa aatagctaga agtattgaaa taataaagtt
     2341 taaatctgat gggtcgcctc aaaaggcgtc tacaatagag atatataata atagattaag
     2401 aaaaactata acaataacac ctgtaagtgg aagagtactt ctgaaagagg gaaaatatga
     2461 aacataaata tggttacttg cttttggaaa gtgtagtatc attgtcttca atggtgataa
     2521 ttatactagt tttatattct atatttttgt ctacaataaa cttaaagtta aaagtagaag
     2581 ataagataga actacaacaa caaagtcttg aaatcataaa atctatggaa ggaattatta
     2641 gcaattctat gggaataatg aatgtatcta attatgaaga aacatttaaa aagacaacgt
     2701 ctataaaatg tagatatgta gatgaaaata ataatgaaga aagcataagt aataaagaaa
     2761 ttatattgaa tgaaagacga aataagttat ttgtaaattc tttaaatggc gaaagtagtc
     2821 aagcaggtgg ttatgaaatt ggagattatg tagatgagat gtatgtatta attactaata
     2881 atggtcaata tgttaatatt aaattgaaat tatcaaaacg tagtcaaaaa tatgagactg
     2941 atttcaaaat aaaagtatgg aattttagtg aaagtatata aaaatacata aatttaagat
     3001 aaagaaatga catcaataag tcatataaaa tataattatt gagaactata taactaaata
     3061 ata
//
LOCUS       DAMJWP010000299         3051 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.306, whole genome shotgun sequence.
ACCESSION   DAMJWP010000299 DAMJWP010000000
VERSION     DAMJWP010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.306"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            97..1539
                     /locus_tag="PC700_003444"
     CDS             97..1539
                     /locus_tag="PC700_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF2582709.1"
                     /translation="MRTIRRVLALGLTLAIFLINVPNVDALTSDTIKGNNIYETAGLI
                     ADKKSYDTAIMVNMDNSIADGLSASGLAGAVDAPILLAQKNKIPNETKQRLKNVKKIY
                     IIGKELSISKSVETELKNTGAQVTRLGGDDRIKTSYSVAKEVNGIKKVDEVILTNAYK
                     GEADTISAAPVSVRDIAPIVLTDGKSVPFSTSNVKTYAVGGSISMSTSLVNKTNAKRL
                     GGSDRYDTNKKVIKEFYPDASEFYLSDGYDLVNALTGSTIAKENPIVLVSESSDKSIL
                     AGADKITRLGSISDSVYNKCVSAAQNNGDSSTKGESPMKNETSILGQPTASLEACLKW
                     AKSKKANDLFIELIPILYDTAVQEGVNPVLAVAQSAKETGFCNFGGVLDASFKNPCGL
                     KTSVGGSDTDKNAHSRFDTWEEGILAQIQHLCLYAGQDGYPLSNPVDPRHEKSLFGKA
                     KTVESLSNNWAGGQYGQDLVRMMGEIEATK"
     gene            1581..1934
                     /locus_tag="PC700_003445"
     CDS             1581..1934
                     /locus_tag="PC700_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582710.1"
                     /translation="MKKSNIFSIIFPTIIMVLMFLTFATDIFNIPDIQSKSIFVVGMV
                     LIFPISFLIQGIICVLNKTNWILSLIVSLVTYIVLMYIFLNDSAYIYVLLYTAFYMIG
                     HITAKLYYKIKTFKN"
     gene            2125..2946
                     /locus_tag="PC700_003446"
     CDS             2125..2946
                     /locus_tag="PC700_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HDF2582711.1"
                     /translation="MNLIDNHIHTNFSSDGKDSMEDTIKKAISIGVRYLTFTDHLEHD
                     EDRGFCINYNDYVPVFNKFKEKYKKDIELLLGVEVGYRKHLKNEIEEIINSNPFDFVL
                     CSTHTIDNVPVPSKAYFKGLSKEDAYYKYFNSILETNREFGDYNIYGHLDYISRYGIY
                     SDNRVIYNDFKDVIDEVLKSIINNGSGIELNTSGYRYGLNAIHPNEDILKRYKELGGF
                     IVTVGSDSHRVEDICKDFDVAYDMLKYLDFKYVSLFKERETYFVNIKKVKSNNIA"
BASE COUNT         1180 a          313 c          540 g         1018 t
ORIGIN      
        1 tataattatt aaattataat aaatgactat agcgtagatg taatactatg ttatagtcat
       61 ttttgaatta aacttgatat atcaaggggg aagaatatga gaacaattag aagagtttta
      121 gcattaggtt taacattagc tatatttcta ataaatgtac ctaatgtaga tgcattaaca
      181 tcagatacaa ttaaaggtaa caatatatat gagacggctg gattaatagc agataaaaag
      241 agttatgata cagcaattat ggtaaatatg gataattcta tagcagatgg tctttcagca
      301 agtggtcttg caggtgctgt tgatgctcca attttgcttg cacaaaaaaa taagatacca
      361 aatgaaacaa aacaaagatt aaaaaatgtt aaaaagatat acataatagg taaagaatta
      421 tcaataagta agtcagtaga aactgaacta aaaaacacag gggcacaggt aactagattg
      481 ggtggagatg acagaataaa gactagttat agtgttgcta aagaggtaaa tggtatcaaa
      541 aaagttgatg aagtgatatt aactaatgca tataaaggag aagcagatac aataagtgct
      601 gcacccgtat cagtaagaga tatagctcct atagtactta cagatggaaa gagtgtgcct
      661 ttttcaacaa gcaatgtaaa aacatatgca gtaggtggga gtatatcaat gagcactagt
      721 ttagttaata aaactaatgc taaaagactt ggtggttcag atagatatga tacaaataag
      781 aaagttataa aagagtttta tccagatgca tcagaatttt atttgagtga tggatatgat
      841 ttagtaaatg cacttacagg ttctacaatt gctaaggaaa atccaattgt attggtgtca
      901 gaaagtagtg ataagtctat attagcagga gcagataaga ttactagatt aggttcaata
      961 agtgacagtg tgtataataa gtgtgtttct gctgcacaaa ataatggcga ttcgtctaca
     1021 aaaggtgagt cacctatgaa aaatgagaca agcatattag gtcagccaac agctagttta
     1081 gaagcgtgct taaaatgggc aaaatctaaa aaagctaatg atttatttat agagttaata
     1141 ccaatattat atgatactgc tgttcaggaa ggtgttaatc ctgttttggc agtagctcaa
     1201 tctgcaaaag aaactggttt ttgtaatttt ggtggagtat tagatgcatc atttaagaat
     1261 ccttgtggac ttaaaacttc tgtaggtggt tctgatactg ataagaatgc ccattcaaga
     1321 tttgatactt gggaagaagg aatattagct caaattcaac atttatgctt atatgcagga
     1381 caagatggtt acccactttc aaatccagta gaccctagac atgaaaaatc tttatttggt
     1441 aaggctaaaa cagttgaaag tttatctaat aattgggctg gaggtcaata tggacaagat
     1501 ttagtaagaa tgatgggaga aattgaggca acaaaataat tgaagattat caaatatcca
     1561 ataaataagg attgattatt atgaaaaaga gtaatatttt tagtatcata tttcctacaa
     1621 tcataatggt gctaatgttt ttaacttttg ctacagatat atttaatatt ccagatattc
     1681 aatctaaaag tatttttgtt gtgggaatgg ttttgatttt tccaatttct tttttaatac
     1741 aagggataat ttgtgtatta aataagacta attggatttt atctcttatt gtgtctttag
     1801 taacttatat agtattaatg tatatatttt taaatgattc tgcatatata tatgttttac
     1861 tttatacagc tttttatatg attggacata taacagctaa actctattac aaaattaaga
     1921 cttttaaaaa ttaaattgaa gtatataaaa gtaacattaa gagaagtatc cttaatgatt
     1981 ttctaaaatc agaaataggt gcttctcttt tttgtaaaaa taaaagtatg taaataaaat
     2041 ttaaccatta taattttctt ttaacaaatg tatacaataa acttgttgta taattaagat
     2101 agataaaata aagggaggga taatatgaat ttaatagata atcatattca tactaatttt
     2161 tcaagtgatg gaaaggattc catggaagat acaataaaaa aagctataag tattggtgtt
     2221 agatatttga cttttacaga ccatttagaa catgatgaag atagaggatt ttgcataaat
     2281 tataatgact atgttccagt atttaataag tttaaagaaa aatataaaaa ggatatagag
     2341 ttattactag gtgttgaagt aggttacaga aaacatctaa aaaatgaaat agaagaaata
     2401 ataaattcta atccttttga ttttgtattg tgctcaactc atacaattga caatgtacct
     2461 gttccatcaa aggcgtattt taaaggtctt agcaaagaag atgcgtatta taaatatttt
     2521 aatagcatac ttgaaactaa tcgtgaattt ggagattaca atatatatgg tcatttggat
     2581 tatatatcta ggtatggaat atattcagat aatagagtta tatacaatga ctttaaagat
     2641 gttattgatg aggtcttaaa gtcaataatc aataatggga gtggtataga gctaaatact
     2701 tctggttata gatatggatt aaatgctata catccaaatg aagatattct aaaaagatat
     2761 aaagaacttg gaggatttat agttactgta ggttctgatt ctcatagggt agaagatata
     2821 tgtaaagatt ttgatgtggc ctatgatatg cttaagtatc ttgactttaa atatgtatct
     2881 ttatttaaag aaagagaaac ttattttgta aatataaaaa aagttaaatc taacaatata
     2941 gcttagagta tgttttgaag taaattaatg gatagttaaa aagttaatag ttataatatt
     3001 gtatcctcta gaaaaatact agaggttttt attttgctaa aataactaaa t
//
LOCUS       DAMJWP010000300         3022 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.307, whole genome shotgun sequence.
ACCESSION   DAMJWP010000300 DAMJWP010000000
VERSION     DAMJWP010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.307"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            144..938
                     /locus_tag="PC700_003447"
     CDS             144..938
                     /locus_tag="PC700_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2582712.1"
                     /translation="MIEVDKLCKSFTRVVKDDKNKSSIRKLKKVKTKKEEFFAVNNVS
                     FKVMEGEIVGILGPNGAGKTTLLRMLGGILTPTSGSINISGYDYSIDRNSAKKEIGYL
                     SGNTKLYGRLSPRELLTTFASLYEMSKEDIEESIENIVKIMDMSEFIDNRIENLSTGQ
                     TQRTSIARCLIHSPKVYIFDEPTLGLDVLSSASIIDFMKNEKLRGKTVLYSTHYMEEA
                     ETLCDKIFMIHNGKIIASGTPQSLKEESGVNNLRDVFIKLARKEDI"
     gene            939..2999
                     /locus_tag="PC700_003448"
     CDS             939..2999
                     /locus_tag="PC700_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2582713.1"
                     /translation="MRSKIVKYIFKKEMLDILRDKKTLFMMIVVPLLLYPLLMLLMIQ
                     VMNMSASSMNSKDINIAFNNKPNKVLVSMIEDDNLEKDTKNTSNDKGKIRVLDVDNYK
                     KSLEENKIDAYIKMEEKKSSINYQIYINSSQENSSNALKRIETVLDKYKRFTIEKTLS
                     EKGLDVQATLEPITYSTVDVAKTEEVAGYFLGQILPFILIIGVLLGSIYPAIDVMAGE
                     KERGTLETLFTLPISNLELVMGKYMAVSLSAIVTAILNILSMGLTMAFILVSGGISSQ
                     FGFGNFNYGDLVLPIITTLICICLFSMVVSAISMCVCSLAKSFKDAQNYITPLMLIIM
                     IPSYVSMIPNIVLDRVTAVIPVVNISLLIKSVLSFKSDLNLIALVLASNVAFVILAII
                     LLSKMFNSEEILFGNSKSFSFLEKRSNIKKGSIPTVSDGVILYAVGLVLLIYVGSLVQ
                     IKFGMAGIIMTQFMIISLPLLFACYIKADFKEVFSLTVPKLKHVFGSIVLWIGGYILI
                     MIITQLILFLFPQNMEVAESLNKALFIKDSVLLNLLAIALLPAICEELFFRGFIFSSF
                     SKSKDKNKSIKLAIICSGVLFGIMHMDFIRIIPTSILGIIFAYSVYKSGSIFVSMLLH
                     FLNNSVAVVLNHYTTGNITNLYKFVEVDFSNLNVLKFALILLISLILIMLGLRVLDRK
                     SLRN"
BASE COUNT         1153 a          316 c          499 g         1054 t
ORIGIN      
        1 gaataaaaaa taataatttg tcagataaac ttgtaaattt ttatgaataa tccattctaa
       61 aacaaaaaac tgtgtataaa aaaggtttat atgttatatt tatataaata taacatatga
      121 acgagaaagg agtggtttga taaatgattg aggtagataa attgtgtaag tcttttacaa
      181 gggttgtaaa agatgataag aataaaagtt caataagaaa gctaaagaaa gtaaaaacta
      241 aaaaagaaga attttttgct gtaaataatg tttcatttaa agttatggaa ggtgaaattg
      301 ttggaatatt aggtccaaat ggtgcaggaa agactacatt acttagaatg ttaggtggca
      361 tattaacacc aacatcagga agtataaata tttctggata tgattattca atagatagaa
      421 attcagcaaa aaaagaaata ggttatctct ctggaaatac aaaactatat ggaaggcttt
      481 ctccgagaga acttctaaca acttttgcaa gtctatatga gatgtcaaaa gaagatatag
      541 aagaatcaat tgaaaatatt gtaaaaataa tggatatgag tgaatttata gataatagaa
      601 ttgaaaatct atcaactggt cagactcaaa gaacatctat agcccgttgt ttaatacatt
      661 ctccaaaagt atatattttt gatgagccaa cactaggtct ggatgtttta agtagtgcat
      721 ctattataga ttttatgaag aatgaaaaac taagaggaaa aactgttctt tactcaactc
      781 attatatgga agaagcagaa actttatgtg acaagatttt tatgattcac aatgggaaaa
      841 taattgcatc aggaacacca cagagtttaa aagaggaatc tggagtaaat aatttgagag
      901 atgtattcat aaaacttgcc agaaaggagg acatttaaat gagaagtaag attgtaaaat
      961 acatatttaa aaaagaaatg ttagacatat tgagagataa aaagacttta tttatgatga
     1021 ttgttgtgcc acttctttta tatcctttac ttatgttatt aatgattcaa gtaatgaata
     1081 tgtctgcaag tagtatgaat agtaaggaca ttaatatagc ttttaataat aaacctaata
     1141 aggtgttagt atccatgatt gaagatgata atttagaaaa agatactaaa aatacttcaa
     1201 atgataaagg caaaataaga gtgttagatg tagataacta taaaaagtca ttagaagaaa
     1261 ataaaattga tgcttatata aagatggaag aaaaaaaatc atctataaat tatcaaatat
     1321 atataaattc ttctcaagaa aattcttcta atgcactaaa aagaattgaa actgttttgg
     1381 ataaatataa aagatttacc atagaaaaaa ctttatctga aaaaggatta gatgtacaag
     1441 ctactttaga accaataaca tatagcactg tagatgttgc aaagacggaa gaagtggcag
     1501 gatacttttt aggtcagata cttccattta tattaataat aggagtttta ctaggttcaa
     1561 tatatccagc aatagatgtt atggctggag aaaaggaaag aggtacatta gaaacattat
     1621 ttacactccc aatatcaaac ttagaactgg ttatgggaaa atatatggca gtttctctta
     1681 gtgcaatagt aacagcaatt ttaaacattt tatctatggg actgactatg gcatttattt
     1741 tagttagtgg aggtattagt agccaatttg gctttggaaa ctttaattat ggagacttag
     1801 tcttaccaat tataactact ttaatttgca tatgtctttt ttcaatggta gtttcagcaa
     1861 taagtatgtg tgtatgttca ttagcaaaaa gttttaaaga tgctcaaaac tacataacac
     1921 ctcttatgtt aattattatg ataccatcat atgtttctat gataccaaat atagtattgg
     1981 atagagtgac agctgtaata cctgtagtaa atatatcttt acttataaaa agtgtattat
     2041 cttttaaaag cgatttaaat ttaatagcct tagttttagc atcaaatgta gcctttgtta
     2101 tattggcaat catactactg tctaagatgt tcaattctga ggaaatactt tttggtaata
     2161 gtaagagttt ttctttctta gagaaaagaa gtaatataaa aaaagggtct ataccaactg
     2221 taagtgatgg tgttatttta tatgcagtag gattagttct attgatttat gttggttctt
     2281 tagttcaaat taaatttggt atggcaggta ttataatgac acaattcatg ataatttctt
     2341 taccattatt atttgcatgc tatattaaag ctgattttaa agaggtattt agcttgacag
     2401 ttcctaaatt aaagcatgtg tttgggagca ttgttctttg gattggtgga tacatactta
     2461 ttatgattat aacacagcta attttattct tatttcctca aaatatggaa gttgctgaaa
     2521 gtttaaataa ggctcttttt atcaaagata gtgttttact gaatttacta gcaatagctt
     2581 tattaccagc tatatgtgaa gaactgtttt ttaggggatt tatattttct tcatttagta
     2641 aatcaaaaga taaaaataag tctatcaaac tcgctataat atgtagtggg gtactatttg
     2701 gtattatgca tatggatttt attagaataa ttccaacatc aatattaggt attatatttg
     2761 catacagtgt gtataaatct ggttcaatat ttgtatcaat gttattgcat tttttaaata
     2821 atagtgtagc agttgtgtta aatcattata caactggaaa tataaccaac ttatataaat
     2881 ttgttgaagt tgatttttcg aacttaaatg tacttaaatt tgccttaatt cttctaataa
     2941 gtcttatatt aattatgtta ggattaagag ttttagatag aaagtcttta agaaattaag
     3001 tataaagtta ttatatagat ta
//
LOCUS       DAMJWP010000301         3014 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.308, whole genome shotgun sequence.
ACCESSION   DAMJWP010000301 DAMJWP010000000
VERSION     DAMJWP010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.308"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            148..738
                     /locus_tag="PC700_003449"
     CDS             148..738
                     /locus_tag="PC700_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2582714.1"
                     /translation="MPKSYSDKEREYIIKRLKEEARLCMEQYGIRKTTVDELVKRVKI
                     PKGTFYLFFQSKELLFFEVLRDIHDSIQKEILYEINRVDESITCEQLTDIFMKFYRMV
                     DSTSILNLMINGEFEILVRKLPDSIIEEHFRHDDFEIGEIISCIPNAKNKDIESFSGA
                     FRVVFLTMLYKREVGSNCFEDALRLMINGLVIQLME"
     gene            852..1697
                     /locus_tag="PC700_003450"
     CDS             852..1697
                     /locus_tag="PC700_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2582715.1"
                     /translation="MIKVDDLSFSYTDRNFLQNINFEVEKGEILGFLGPSGAGKSTLQ
                     KILIGMITNYGGSVIVNGVESKRHSNKFYENIGVDFEFPSLYEKLTAIENLKYFGSLY
                     SKKLLSIDELLKSVGLENEANKRVSEYSKGMKSRLNFIKALLHNPDILFLDEPTSGLD
                     PSNSKVMKDIILSEKSKGKTIILTTHNMLDATELCDRVAFIVNGKISALDTPHNLIMS
                     KGAIKVRYTYFDNGEKTSECFLNNTANDKNLNMLIKKNKLLSIHSSEPTLNDIFIEIT
                     GRNLQ"
     gene            1694..2371
                     /locus_tag="PC700_003451"
     CDS             1694..2371
                     /locus_tag="PC700_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2582716.1"
                     /translation="MRLKNLILGDIKFQFKYGFYFLYLFLSIIYICIINVFPTFMREK
                     IAIIMIYSDPAAMGLFFMGAIVLLEKSQRVLNSLAVSPVKVSEYILSKVISLGVISSI
                     VAMFIAITLNLDNIIISTIGTFFSSIIFSLLGLILASKASSLNQFIVLSIPIEIICFI
                     PPILNVLLDTKSYANLYPFNICISLISGDKNFIMINILISIIIIIYFITYYFICSSWK
                     KVGGVKL"
     gene            2368..>3014
                     /locus_tag="PC700_003452"
     CDS             2368..>3014
                     /locus_tag="PC700_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2582717.1"
                     /translation="MIKIINAFKQEFEQIKRDAMLFIVCVSPILCGVFIKFGIPLIQN
                     ISLNKFYYQLNLEPYFLMFDLLLAFITPFMFFFASTMVILGEIDDSISRYLIVTPLGK
                     TGYLISRFGIPGTLAFIITMILLIFFSLTKISFLLNLSISLLVLLQGVIISILVISLS
                     SNKLEGMVITKFSGVFMMGILAPFFILNKAQYILFFLPTFWISKAFKEDNYIYMF"
BASE COUNT         1117 a          312 c          457 g         1128 t
ORIGIN      
        1 ctagataata aaaaatgcta tataaaattc agaattttcg aaagttttta agcttatttt
       61 taaatatact aataatacta ttgacattag atagaaaaag tattatattg actatataaa
      121 ttattttagt caaactggag gaaaaagatg cctaaatcgt attctgataa agaaagagag
      181 tatataataa aaagacttaa agaagaagca agattgtgta tggagcaata tggtattaga
      241 aaaactactg ttgatgagct tgtaaaacgg gtaaaaatac caaaaggaac tttttatcta
      301 ttttttcaat caaaagaatt gctctttttt gaggtattga gagatataca tgactctata
      361 caaaaagaaa tcttatatga gataaataga gtagatgaaa gtattacatg tgaacaattg
      421 acagatatat ttatgaaatt ctataggatg gttgatagta cttcaatttt aaatctcatg
      481 ataaatggag aatttgagat attggtgaga aagttaccag attctataat tgaagaacac
      541 tttagacatg atgactttga aattggagag attatatctt gcataccaaa tgcaaaaaat
      601 aaagacatag aaagctttag tggagcattt agagtagttt ttcttaccat gctatataag
      661 cgcgaggttg gaagtaattg ttttgaagat gctttaagat taatgataaa cggattagtt
      721 atacaactta tggaatagaa tttaaatttt gtttgctaat aacttttatt aaaaattatt
      781 aacaaacaaa aaaattatat tttattgacc atatagtcaa aatagtcaaa aatttgaaag
      841 ggtgatattg aatgattaaa gttgatgatt tatcatttag ttatacagat agaaattttt
      901 tacaaaatat taactttgaa gtagaaaaag gggaaatact aggattttta ggaccatcag
      961 gtgcaggaaa atctacatta caaaagattc taattggaat gataactaat tatggaggaa
     1021 gtgtcattgt aaatggagta gagagtaaaa gacactcaaa taaattttat gaaaatatag
     1081 gagttgattt tgaatttcct agtttatatg aaaagttgac tgcaatagaa aatttaaaat
     1141 attttgggtc tctatattct aaaaaactac tatctattga tgagttatta aaatcagtag
     1201 gactagaaaa tgaagcaaat aaaagggtat ctgagtattc aaaaggtatg aaatctcgat
     1261 taaattttat aaaagcttta ttacataatc cagatatttt atttttagat gaaccaacaa
     1321 gtggtcttga cccgtcaaat agcaaggtta tgaaagatat aattttatca gaaaaatcta
     1381 aaggtaaaac tattatttta acaactcata acatgttaga tgcaacggag ctttgtgaca
     1441 gagtagcgtt cattgtaaat ggtaaaatat ctgctttaga tacaccacat aatcttatta
     1501 tgtcaaaagg agctattaaa gttagatata cttactttga taatggagaa aaaacttctg
     1561 aatgttttct gaacaatact gctaatgata aaaatttgaa tatgcttatt aagaaaaata
     1621 aacttttatc aatacatagt agtgaaccaa cattaaatga tatttttatt gagataacag
     1681 ggaggaattt acaatgaggc taaaaaatct tattttagga gatataaagt ttcaatttaa
     1741 gtatggtttt tattttttat atcttttttt aagtattata tatatttgta ttatcaatgt
     1801 atttccaact tttatgagag aaaaaatagc tataattatg atttattctg acccagcagc
     1861 aatgggattg ttttttatgg gagctattgt acttcttgag aaaagtcaaa gggttttaaa
     1921 ttccttagct gtatctccag taaaagtatc tgagtatatt ttatcaaagg taatttcatt
     1981 aggagtaata tctagtattg tagctatgtt tattgctatt acattaaatt tagataatat
     2041 tataatatca actataggta cctttttttc ttctattata ttttctttgc ttggattgat
     2101 acttgcaagt aaagcatcta gtttgaatca gtttattgtt ttaagtattc ctattgagat
     2161 tatatgcttt attccaccaa tactcaatgt acttcttgac acaaaatcat atgcaaattt
     2221 gtaccctttt aatatatgta tttctcttat tagtggagat aaaaatttta taatgattaa
     2281 tattttaata tcaattataa taataattta ttttataact tattatttta tttgtagcag
     2341 ttggaaaaag gtaggtggag taaaactatg ataaagatta taaatgcgtt taaacaagag
     2401 tttgaacaaa ttaagagaga tgctatgctt tttatagtat gtgtttctcc aatattatgt
     2461 ggagtattta taaaatttgg aataccatta atacagaata tatcacttaa caagttttat
     2521 tatcaattaa atttagagcc gtatttcttg atgtttgatt tacttttagc ttttattaca
     2581 ccttttatgt tcttttttgc ttctacaatg gtcatattgg gagaaattga tgacagcatt
     2641 tcaagatatt taatagtaac tccgcttggt aaaactggtt atcttatttc acgatttgga
     2701 ataccaggta ccttagcatt tattattact atgattctac ttatcttttt ttctctaact
     2761 aaaatttctt tcttgttaaa cttatcaatt tctttattag ttttattaca aggagttatt
     2821 atatcaatac tagtgatttc tttatcatca aataaactag aaggaatggt aattacgaaa
     2881 ttctcaggtg tttttatgat gggtatttta gcaccatttt ttattttaaa taaagcacaa
     2941 tatattctgt tcttcttacc aactttttgg atatcgaagg catttaagga agataattat
     3001 atctatatgt ttat
//
LOCUS       DAMJWP010000302         3007 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.309, whole genome shotgun sequence.
ACCESSION   DAMJWP010000302 DAMJWP010000000
VERSION     DAMJWP010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.309"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     repeat_region   114..1068
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=313..341
                     /rpt_unit_seq="atttacataccacatagttaatataaaac"
     gene            complement(1264..1503)
                     /locus_tag="PC700_003453"
     CDS             complement(1264..1503)
                     /locus_tag="PC700_003453"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582718.1"
                     /translation="MECYLKRTLIIFSKAFLSISFKTRKYLFIVIFILVCPNLSEMIF
                     ILVPARRRIIRIDNIVSIYKYVGVLKLINYSILIL"
     gene            1715..2986
                     /locus_tag="PC700_003454"
     CDS             1715..2986
                     /locus_tag="PC700_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF2582719.1"
                     /translation="MLKVLKGNIIFTKTSETFTVFENSYIILEKGKVKGIFKEIPKEY
                     KNLKVVDYTDKLIIPGMNDLHAHASQFKNLGMAMDKELLPWLETYTFPEESNYKDIEY
                     ATKMYKKFVKELWKSGTTRIAVFATVHKEASMKLMDIFKEVGLGAYVGKVNMNMNCPD
                     TLLENTQDSIADTEEIINKYSDTDSIVKPIITPRFIPSCTSELLKGLGDLCLKYNIPM
                     QSHLSENYDEIEWVRSLEPESKFYGDAYNRYNLFGQTPTLMAHCVHCSQDEIDLMREN
                     NVMAVHCPASNFNVGSGAMPIRKLIDNNIRLALGSDISGGHTLSIFKAMVSAIQLSKL
                     YWVNSGKKYNFLSLSEAFYIATKSGGSFFGKVGSFEEGYDFDALIIDDSNLNHDNYSI
                     LERLERFIYVGDDRNIIHRYVCGKLIEEPNI"
BASE COUNT         1192 a          409 c          388 g         1018 t
ORIGIN      
        1 ctaaagtcaa tgttatctgg taattattta ctacaaatga taaaaaataa ttatatttta
       61 aagtagaaaa tgctgtataa attattgggg tattttacct atacttgttg caaatttaca
      121 taccacgtag ttaatataaa acaaatttca agcgattgga caacgaataa aaattgcaat
      181 ttatatatca tgtagttaat ataaagcata tttgtgtcga tatatagttc taactaagta
      241 taagatttac atacctcata gttaatataa aacaatgtgt ccgaattgga cacatagaaa
      301 ggaaaagtaa aaatttacat accacatagt taatataaaa ctatgcttat atattatgtt
      361 tattctgttg tttctaaaat ttacatacca catagttaat ataaaacgat tttttcatgc
      421 ggaggaaaat tgatgtgggg acatatttac ataccacata gttaatataa aacagataat
      481 ctaggcacac atacaatgcg caaaacttta tttacatacc acatagttaa tataaaacat
      541 tcagacgata taggcgcagg agctaagtat tctgaaattt acataccaca tagttaatat
      601 aaaaccagca cgccaagtat aagtaaaact tccactatca ccatttacat accacatagt
      661 taatataaaa ctaaatgttg ctccgtaacc taaaaacata atgctccaat ttacatacca
      721 catagttaat ataaaacgtg aaagaaaaat taatatgcta tttacatttt acaaatttac
      781 ataccacata gttaatataa aaccattcca caatttatac aattttttct gttctaaaac
      841 atttacatac cacatagtta atataaaact cttcttatct tccagttgca aaatctatgc
      901 ttgacctatt tacataccac atagttaata taaaactagt ttgagtggta aaagtacgaa
      961 gtacccctct ttaatttaca taccacatag ttaatataaa acatatgtaa ttcaccgttt
     1021 taaattatag ataaattaaa tttacatacc acatagttaa tataaaaccc caaaataaac
     1081 taagtatttt cagtacttac acacatacaa ctctctcaaa tttgcagtga accatgagta
     1141 gtgcaattga taacatttat catacaccct caatccctta cattgcaact gttaaaccct
     1201 actttatcaa aaatatcgct cactgcaaaa tttatatatt tttattatat cataaaattc
     1261 aaactataaa attaaaatgg aataatttat caatttaaga acacctacat atttgtaaat
     1321 acttactata ttatctattc taataattct tcttctagct ggaactaata taaaaatcat
     1381 ctctgaaaga ttaggacata ctaatataaa aataactatg aatagatatt ttcttgtttt
     1441 aaacgatatg gataaaaaag cttttgaaaa tataatcaaa gttctcttta aataacattc
     1501 cattttatgt aagtaagcat tatatttttc ttaaatgtca cttaagtgtc agttatatat
     1561 aataatagta aacaactgta tttcactaat atttgctatt atctaatagt atttatagtt
     1621 tagatttgat ataatacatg tggtaaaatt ttgatttgta aaacaaaatt ttacctacat
     1681 atattaaaac aaatttaaaa ctaaggagac ctatatgtta aaagtattaa aaggaaatat
     1741 aatttttaca aaaacttcag aaactttcac agtatttgaa aatagttata ttatactgga
     1801 aaaaggtaaa gtaaagggaa tatttaaaga gatacctaag gaatacaaaa acctaaaagt
     1861 agtagattac actgataagc ttataatacc tggtatgaat gacctccatg cccatgcctc
     1921 tcaatttaaa aatcttggaa tggctatgga taaagagctt ttgccatggc ttgaaactta
     1981 tacatttcca gaagagtcaa attataaaga tatagaatat gcaacaaaaa tgtataaaaa
     2041 atttgtaaaa gaactttgga aatctggaac tacaagaata gcagtgtttg caacagtaca
     2101 taaagaagcc tctatgaaac ttatggatat attcaaagaa gttggtcttg gagcatacgt
     2161 tggtaaagta aatatgaata tgaattgccc tgatacttta ttagaaaaca ctcaggattc
     2221 tattgctgat acagaagaaa ttataaataa gtatagtgat acagattcta ttgtaaaacc
     2281 aataataaca cctagattta taccaagctg tacaagtgaa cttttaaaag gtttaggaga
     2341 tttatgttta aaatataata ttcctatgca atcacactta tctgaaaatt atgatgagat
     2401 tgaatgggtt agaagtctag aacctgaatc taaattctat ggagatgcat ataatagata
     2461 taatttattt ggtcaaactc ctactttaat ggctcattgt gttcattgtt ctcaggatga
     2521 aatagattta atgagagaaa acaatgttat ggcagttcac tgtccagctt ctaactttaa
     2581 tgttggtagt ggtgctatgc ctatcagaaa attaatcgat aataatatta gattagcact
     2641 tggatcagat ataagcggtg gacatacatt gtcaatattt aaagcaatgg tatctgcaat
     2701 acaattatct aaattatatt gggttaattc tggtaagaag tataatttct tatctttatc
     2761 tgaggctttt tatattgcaa ccaaaagtgg tggaagtttc tttggaaaag ttggtagctt
     2821 tgaagaaggt tatgattttg atgcattgat tattgatgat tcaaatttaa atcatgacaa
     2881 ttattctata cttgaaagac ttgaaagatt tatctatgtt ggtgatgata gaaatatcat
     2941 tcatagatat gtatgtggaa aactaattga agaacctaat atataattat aataaaaatt
     3001 ttaatta
//
LOCUS       DAMJWP010000303         2974 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.310, whole genome shotgun sequence.
ACCESSION   DAMJWP010000303 DAMJWP010000000
VERSION     DAMJWP010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.310"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(35..643)
                     /locus_tag="PC700_003455"
     CDS             complement(35..643)
                     /locus_tag="PC700_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HDF2582720.1"
                     /translation="MEKDFFRKNGIDLAKSILGKYLIRKYENKVIVTKIIETEAYMGV
                     NDKGAHVYGNKKTDRTKPLYLDGGHIYVYLIYGMYNCLNLSANIENVPECVLIRGVEP
                     ITSLDEISMNRYNKAYTELSKYQVKNITNGPGKLCKALKIDRSLNSKSIMGEELYISD
                     FYYDDKGKKVFSKDELDIKTSKRINIDYAEEAKDFLWRFYIE"
     gene            complement(752..>2974)
                     /locus_tag="PC700_003456"
     CDS             complement(752..>2974)
                     /locus_tag="PC700_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HDF2582721.1"
                     /translation="KLIKDLTYFVFFTTIICFVAISKINIVDSDRLIGVIYLLLCNHT
                     YYVYVYSLNNRVVYPYYELDSINKKLSKKSEQLGKINLAIEKDMIIQRTLKNYIDQRK
                     ELLRQALDTIPNVWIVTDYDFNISYTNNKFKDEFSKDMNNYYKILTFIKEDEKVAEKL
                     KKFDNDISIIDKLVELNDKIYLLSLSNNKDESNYLISLNDITNEIKIDEEIRNINKDY
                     ENIILNIPSPILVRSAEGGIKKNKVISINKKYEKSFKCVSSDLVDMTLEQYFETFNID
                     FFDNRKFKRLNLTQEKKAEIVSYNDTANCIVNFVMSDSEGEEHVEEVRVGDYWSDNKL
                     FKLLTFRDITKEINILRTVNEQKIIYEKLLDVIPEAIFLEDLSTSRVLYTNRAFRELF
                     GISSDVLGVTTQKYRNILVKKYINNLNIGEREKSIHIVNENNHIKEVKMISRTLYFGQ
                     KRSRVRIIKDLSVQRESERLKKALIKQRQYDQMKMEFYANISHELKTPLNNIYSSVQL
                     IENLYKKGKIIDFQDILKEHIKITKQNMFRLLRLIDNIINISQVKSDIYKIKAVNFDI
                     IDITERIVTSISSYAKSKGIDLIFDTDEETVMVGLDPESIERIILNILSNAIKFTLEG
                     GEILVGIYKKDETVEIIIKDTGVGIDKEKLNDIFNRFKQIENSGISNEFGSGIGLCLT
                     KSLIEIQNGKIYIDSKVGEGTNVKVIFPIKEVVEEVYDNSNYNDNIEKFEIEFFDIYK
                     "
BASE COUNT          939 a          483 c          237 g         1315 t
ORIGIN      
        1 ttcctataga tacatatctt acctatataa aaacttattc tatataaaaa cgccataaaa
       61 aatcttttgc ttcttctgca taatctatat ttatacgttt actagttttt atatctaact
      121 catctttact aaatactttt tttcctttat catcataata aaaatcactt atatataatt
      181 cttcacccat tatactctta ctatttagac ttctatctat ttttaaagct ttgcaaagtt
      241 ttcctggtcc gttagtaata ttttttactt gatatttact caactcagtg taagctttgt
      301 tgtatctatt catagatatc tcatctaaag aagttatagg ctctacccct cttattaata
      361 cacactctgg cacattttct atatttgcag ataaatttaa gcaattatac ataccataaa
      421 ttaaataaac atatatatgt ccaccatcta aatataatgg cttagttcta tctgtctttt
      481 tattgccata tacatgtgct cctttatcat ttacacccat gtatgcttct gtctctatga
      541 ttttggtaac aattacttta ttttcatatt tccttattag atatttacct aatattgatt
      601 tggctaaatc aattccattt tttctaaaaa aatccttttc caaaatttca cctcataaaa
      661 tttatttaaa acgcatcaaa tacaactaaa gtaataaata aaattaactc ttattaatgt
      721 gatatatata aacaataaat ttagccattt attatttata tatatcaaaa aattctatct
      781 caaacttttc aatattatca ttgtaatttg aattatcata tacttcttca acaacctctt
      841 taattgggaa tataactttt acattagttc cttctcctac cttactatct atgtaaatct
      901 tcccattttg aatttcaatc aatgattttg taaggcataa acctatacca cttccaaatt
      961 catttgatat accactgttt tcaatttgtt taaatcgatt aaatatatca tttaattttt
     1021 ctttgtctat gcccactcca gtatctttta taattatctc tactgtttcg tcttttttat
     1081 atatacctac aaggatttct cctccttcta aagtaaattt tatagcattt gataatatat
     1141 ttagtattat tctttctata gattctgggt caagccctac cataacagtt tcttcatccg
     1201 tatcaaaaat taaatctatt cctttagact tagcataact actaatagat gtaactattc
     1261 tttctgttat gtcaataata tcaaaattaa ctgctttaat cttatatatg tctgacttaa
     1321 cttgagaaat atttattata ttatctatta atctcaataa tctaaacata ttttgtttag
     1381 ttatttttat atgttctttt agtatatcct gaaaatcaat tatttttcct tttttataaa
     1441 ggttttcaat aagttgaact gaactatata tattatttaa aggtgtctta agttcatgag
     1501 atatattagc ataaaactcc atcttcattt ggtcatactg tctttgtttt attaatgctt
     1561 ttttaagtct ttcagactct ctttgcacac ttaaatcttt aataattctc actctacttc
     1621 ttttctgtcc aaagtataat gttcttgata tcatttttac ttctttaatg tgattatttt
     1681 catttactat atggatactc ttctctcttt ctcctatatt taaattgttt atgtattttt
     1741 ttactaatat atttctatat ttttgtgttg taactcctaa tacatcacta gaaattccga
     1801 acaactctct aaaggctcta tttgtatata gtactcttga tgtacttaaa tcctcaagaa
     1861 aaatagcctc tggtataaca tcaagtaatt tttcatatat tattttttgt tcatttactg
     1921 ttctaagaat atttatctct ttagttatat ctctaaatgt aagcaattta aagagtttat
     1981 tatctgacca atagtctcca actctaactt cttcaacatg ttcttcacct tctgagtcag
     2041 acatcacaaa attaactata cagtttgctg tgtcattgta acttactatt tcagctttct
     2101 tttcttgtgt tagatttaat cttttaaatt tcctattgtc aaagaaatct atgttaaaag
     2161 tttcaaaata ttgttctaat gtcatatcga ctaaatcact tgaaacacat ttaaaactct
     2221 tttcatattt tttatttata cttattacct tattcttctt tatgccacct tcagcacttc
     2281 ttacaagtat aggagatgga atatttagta taatgttttc atagtcttta tttatatttc
     2341 ttatttcttc atctattttg atttcatttg taatatcatt taaacttatt aagtaattgc
     2401 tttcatcttt attattactt aaacttaaca aataaatttt atcatttaac tctaccagtt
     2461 tatcaattat acttatatcg ttatcaaatt tcttcaattt ttcagcaact ttttcatctt
     2521 cctttataaa agttaatatt ttataataat tattcatatc ctttgaaaat tcatctttaa
     2581 atttattatt tgtgtaactt atattaaaat catagtctgt tactatccat acatttggta
     2641 ttgtatccag agcttgtcta agtaattctt ttctctggtc tatgtagttt tttaatgttc
     2701 tttgtataat catatccttt tctattgcta aatttatctt tcctaattgc tctgactttt
     2761 tacttaattt tttattgatt gaatcaagtt cataatatgg atacactact ctattattaa
     2821 gactgtatac atatacataa taagtatggt tacataatag taaatatatt actcctatta
     2881 atctatctga atctactata ttaatcttag aaattgctac aaaacatata atagttgtaa
     2941 aaaaaacaaa atatgttaaa tcttttatta actt
//
LOCUS       DAMJWP010000304         2969 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.311, whole genome shotgun sequence.
ACCESSION   DAMJWP010000304 DAMJWP010000000
VERSION     DAMJWP010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.311"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            120..488
                     /locus_tag="PC700_003457"
     CDS             120..488
                     /locus_tag="PC700_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2582722.1"
                     /translation="MENLVKIFKALSDETRLNILILVSKRNICQKGISKYLGISDSAV
                     SQHIKILKDVGIITGYKEGYYVLYHINKESFNICVDFINSMLSNSSESFIDVFDVNTI
                     HLGCSKECKSIKKCCKRREK"
     gene            491..862
                     /locus_tag="PC700_003458"
     CDS             491..862
                     /locus_tag="PC700_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HDF2582723.1"
                     /translation="MKVCIPVEENKGLDSKPYGHFGSAPIFVVCDLESGEVKSLDNGD
                     LDHEHGKCQPLKALSGTAVDAVVVGGIGQGAIIKLNGMGIKVYRAEGDTISANLDLLK
                     NGKLVEFPSDHTCSHDGCGHH"
     gene            complement(1006..1461)
                     /locus_tag="PC700_003459"
     CDS             complement(1006..1461)
                     /locus_tag="PC700_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582724.1"
                     /translation="MNINTVQGDSIEVLLRQLGATRISKVSSTLYFIKFDLGDGWEIS
                     YTYNINAKDQYFLQRIEPYPIGRGLFNDEYEIVSFISKDLKKFLNAKNSSNFKTFVEV
                     TRKVNSIIDNVEELFLNYNVDGDDLKTLNKELNDILNDIDYIEKHTKKI"
     gene            complement(1500..2765)
                     /locus_tag="PC700_003460"
     CDS             complement(1500..2765)
                     /locus_tag="PC700_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HDF2582725.1"
                     /translation="MEFQQIIAICIFLIVMAAIITEKVNRSVAAVGGALLMIIFNILT
                     LDEGLSHIDFNTIGVLVGMMLFVAVVKNSGLFEYIAIWTAKKAKGDPWKIMICFAIIT
                     AILSAVLDNVTTVLLIGPMTIVITQILGLNPVPFLITQILASNIGGTATLIGDPPNIM
                     IGSAANLSFMDFVINLGPAVIVILAITIICFRFIYGKELVVNERAKNAILKLDEKKSV
                     KDKPLLIKSLILIAFILFGFMFHSTIHIDSSVVALTGASIMLLIGKQDVDEIMAGIEW
                     STILFFMGLFVVVGGLVEVGIINKLAQALIGLTEGHLVFTMLLILWLSAIVSSFLDNI
                     PFVATLIPLILTMQAEGIDVMPLWWATSLGACLGGNGTLIGASANVVLAGIGNKHGHP
                     ISFKEYFKIGFPLMIISIIISTVYLIIKF"
BASE COUNT         1099 a          409 c          412 g         1049 t
ORIGIN      
        1 tattagtaga aattggaatt atttataata tactccaaaa tttaaaaata atggttgaaa
       61 aatctaatta agagtactat aatttaatta agtaattact taatcaaaac gaggtactta
      121 tggaaaactt ggttaaaata tttaaagcat tgagtgatga aactagactt aacatactta
      181 tattagtatc caagagaaat atatgtcaaa aggggatttc taaatatcta gggatttcag
      241 attctgctgt ttcacaacat ataaaaatat taaaagatgt tgggataata acaggatata
      301 aagaaggata ctatgtttta tatcatatta ataaagaatc atttaatata tgtgtagatt
      361 ttataaattc tatgttaagt aattctagtg aaagctttat agatgtattt gatgtaaata
      421 caatacatct aggatgcagt aaagaatgta aatctataaa aaaatgttgt aagagaaggg
      481 agaaataata atgaaagtat gtataccagt agaggagaat aaaggattgg atagtaaacc
      541 atatggacat tttggttctg ctccaatttt tgttgtgtgt gatttagaaa gtggagaagt
      601 taaatcttta gataatggag atttagatca tgaacatgga aaatgtcaac cattaaaagc
      661 attgtcaggg acagctgtag atgcagtagt tgttggagga ataggacaag gagctataat
      721 taaattaaat ggaatgggaa taaaagttta cagagcagaa ggtgatacta taagtgctaa
      781 tttagactta cttaaaaatg gaaaattagt agagtttcca tcagaccata cttgctcaca
      841 tgacggatgt ggacatcatt aaaaaaaaca gctgatgaaa tatatacttc atcagctatt
      901 ttttctttta ataaatacga ttttattctt ttaaaaccaa aatttagatt tttatgtttt
      961 ttgtagtatc cttaataata tttcaagttt acaagtttac tacttttaga ttttttttgt
     1021 atgtttttct atatagtcaa tatcatttaa tatgtcattg agttctttat taagtgtttt
     1081 taaatcatca ccatctacat tgtagtttaa aaatagttcc tctacattat caatgataga
     1141 attgactttt ctagtaactt caacaaatgt cttaaagtta ctactatttt tagcatttag
     1201 aaattttttt aaatcctttg atataaatga tactatttca tattcatcat taaatagacc
     1261 tctaccaata ggatatggtt caattctctg taaaaaatat tggtctttag cattaatatt
     1321 ataagtataa gatatttccc aaccgtctcc taaatcaaac ttgataaaat ataatgtact
     1381 acttactttt gatattcttg tggcaccaag ctgtcttaaa agaacttcaa tcgaatcacc
     1441 ttgaacagta ttaatattca tatatacaca tcccctttag tattattata ctatattttt
     1501 taaaatttta taattaaata aacagtagat ataataatag atataatcat aagtggaaaa
     1561 cctattttga aatactcctt aaaagagata ggatgaccgt gtttattacc tatacctgct
     1621 agtacaacat tagcagatgc tcctattagt gttccatttc ctccaagaca agctccaaga
     1681 gatgttgccc accatagtgg cattacatca ataccttcag cttgcatagt caaaataaga
     1741 ggaattagtg ttgcaacaaa tggaatatta tctaagaaag aagagactat agctgataac
     1801 cataatatca acaacattgt aaatacaaga tgtccttctg taagtccaat caatgcttga
     1861 gccaatttgt ttattatccc aacttctact agtccaccaa ctactacaaa taatcccata
     1921 aagaataaaa ttgtggacca ttcaatacct gccataattt catctacatc ttgttttcct
     1981 attaaaagca ttatagatgc tcctgtcagt gctacaacag aagaatctat gtggattgta
     2041 ctgtgaaaca taaaaccaaa aagtatgaat gctattaata taaggctttt tataagtaga
     2101 ggtttatctt taacagattt tttttcatct agttttaata ttgcattttt agctctttca
     2161 ttcacaacaa gttcttttcc ataaataaat ctaaagcata ttatagttat agcaagtatt
     2221 actataacag caggacctaa atttattaca aaatccataa aactaagatt ggcagcacta
     2281 ccaatcataa tatttggagg gtctcctata agagtagctg ttcctcctat atttgaagct
     2341 aaaatctgtg ttattaaaaa tggaactgga ttaagtccta atatttgtgt aataactata
     2401 gtcattggtc caataagtaa gacggttgta acattatcta atacagctga taaaatagct
     2461 gttataatag caaaacaaat cattatcttc catgggtcac ctttagcttt ctttgcagtc
     2521 cagatagcta tatattcaaa taaccctgag tttttgacaa cagctacaaa tagcatcata
     2581 ccaactaata caccaatagt attgaaatct atatggctta atccttcatc aagtgtaagt
     2641 atattgaata ttatcattaa tagtgcacca ccaacagctg ctacggatct gtttaccttt
     2701 tctgtaataa tagctgccat aactattaaa aatatacata tagctattat ttgttgaaat
     2761 tccataatat aagtacctcc atttgtatta ttttgtgcct aatgctagta ctatatatag
     2821 tattactttt ttattgtttt ttcaatggat gtatgaaagt atattattta acaaagtttt
     2881 tgagaaattg acagaattta aagaaaattt ttgttattta aataaatttt aaaattattc
     2941 cattgataat aatattaaac gacaaacga
//
LOCUS       DAMJWP010000305         2952 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.312, whole genome shotgun sequence.
ACCESSION   DAMJWP010000305 DAMJWP010000000
VERSION     DAMJWP010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.312"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            203..1387
                     /locus_tag="PC700_003461"
     CDS             203..1387
                     /locus_tag="PC700_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582726.1"
                     /translation="MQKSVKKAKVTGSMLAIFGVASVLFSSHAGGGFATGNQETQYYV
                     QYGWTAPLMAILAMIILTATMREVIIMYNNNNCRNYKDLFCELWRPYPKLEIIWEIYY
                     YLMVLIAVSAVIAGAAAVFQSIGVNYFVAVFIIGVVLLVFTIFGAMLVSKAATAMTIA
                     ILVCTLTIFIVGIKAKVPEITEILSNRTSFTPGYIKPILNTFIYAGFQSVVIPTLAGC
                     SRPLLKNSKEATKAMIISFVMNAIALGLAVTMLMGWYREIIAAGQTTLPTLYVAGQSG
                     NHTIYIIYNVALFLCLMSTGVTTIFGLVNRFEDHKALSFLSSRMKRRVFTACAIMVVS
                     MLISLTGLSNIVKYGYGYCGYLGLFTIVIPFLTLGHYKNKKFAKENPEAKWPAELNEN
                     VN"
     gene            complement(1764..2873)
                     /locus_tag="PC700_003462"
     CDS             complement(1764..2873)
                     /locus_tag="PC700_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2582727.1"
                     /translation="MDFKSLGISENTINILKKSGITTPTPIQKESIKLIKEGKDVIAE
                     AQTGTGKTLAFLLPIFENISLDINDIQVLILSPTRELAIQITEEAMKLKESKDVSILA
                     AYGGKDIGSQIKKLKGNIHMIIATPGRLLDHLNRKTIDLSKLKTFVLDEADQMLLMGF
                     KNEVEAILKETSNKKQTLCFSATINSQVKKLAYRYTKNPVVVSIQKEEITLNNIKQEV
                     VETTDRKKLDALCKVLDEDNPFMAIIFCRTKRRVDNLEEALAIRGYNCQKLHSDIAQS
                     KRERIMKSFRNLDIQYLIATDVASRGLDISGVSHIYNYDLPETPEDYIHRIGRTGRAG
                     EEGYTCAFIDPKNERMLSEIETAIESKISRRIIEL"
BASE COUNT          981 a          407 c          447 g         1117 t
ORIGIN      
        1 aaattaaaaa acaaagtgaa tttgaatact atttaaaaaa taaaaaaata tataaaaaat
       61 atttaataca aaggtagaaa taagttgaaa atgtaaagtc attggaaaaa tatagttgaa
      121 tttaatgaac aaaaatgtta ttgttttatc aatgaagtgt taaaataaaa actttcaata
      181 tcattttagg gaggatgatt tcatgcaaaa aagtgtaaag aaagcaaaag taacgggatc
      241 tatgttagcc atttttggtg ttgcaagcgt tctttttagt tcacatgcag gaggaggatt
      301 tgctacaggg aatcaagaaa ctcagtacta tgtgcagtat ggatggacag caccacttat
      361 ggcaatttta gcaatgatta tacttactgc taccatgaga gaagttatca taatgtataa
      421 taataacaat tgtaggaatt ataaggattt attttgtgag ctttggagac catatccaaa
      481 acttgaaata atatgggaga tatattacta tctaatggtt ttaatagctg taagtgctgt
      541 tatagcaggt gcagcagcag tatttcaaag tataggagta aattattttg tagctgtatt
      601 tattattgga gttgtacttc ttgtctttac tatatttggt gctatgcttg tatctaaagc
      661 agctacagca atgacaatag ctattctagt atgtactctt actattttta ttgttggtat
      721 aaaagctaaa gtaccagaaa taactgaaat attatcaaat agaacgagtt tcacacctgg
      781 ctatatcaag ccaatactta atacttttat ttatgcaggt tttcaatcgg ttgttatacc
      841 tacacttgca ggatgttcaa gaccacttct taagaactca aaagaagcta ctaaggcaat
      901 gataatttca tttgttatga atgcaattgc attaggactt gctgtaacta tgctaatggg
      961 atggtataga gaaattattg ctgctgggca aacaacactc cctactcttt atgttgcagg
     1021 tcaatctgga aatcatacta tatacataat ttataatgtt gcattattcc tttgtttaat
     1081 gtctacagga gtaactacta tatttggtct tgtaaataga tttgaagacc ataaagcact
     1141 ttctttcctt tcttcaagaa tgaaaagaag ggtatttaca gcttgtgcaa taatggtagt
     1201 gtcaatgctt atatcactta caggattaag taatattgtt aaatatggat atggatattg
     1261 tggttatctt ggtctattca ctatagtaat tccattcctt acactaggac attataagaa
     1321 taagaagttt gcaaaggaaa atccagaagc taaatggcca gcggaattaa atgaaaatgt
     1381 aaattagatg tagtagcaaa aaatattgct aaagagaata ctataaataa gaatactata
     1441 gataatatat ctgcatgttt attaagatta tttgcaaaat ctaatattga acaactataa
     1501 acaagcaata taaaactaag aataaaagat aaaaagaggg tgtctcaaaa tggactttta
     1561 gtttataagg cactcttttt tatatgaagt caaatatatt ttcattattt tgacaataaa
     1621 agagactaat cctactttgg atagcctctt ttttctttaa tataaaatag tataacagca
     1681 aagtatagat tgatacatgt tttctgtagc tttctatata ttacagtgtt tgtgtggcat
     1741 tggtatttta agcaatctat aaattataat tctataattc ttctactaat tttagattct
     1801 atagccgtct ctatttcact taacattcgt tcatttttag ggtcaataaa agcacatgta
     1861 tagccttctt caccagctct accagttcta ccaatacggt ggatgtagtc ttctggtgtt
     1921 tctggtaaat cgtaattata tatatgactt actccagata tatcaaggcc acgagaagct
     1981 acatctgtag ctattaagta ttgtatatct aaatttctaa atgatttcat tattctttct
     2041 cgttttgatt gagcaatatc actatgaagt ttttgacaat tatatcctct tatagcaaga
     2101 gcttcttcaa gattgtcaac ccttctctta gtcctacaaa atattattgc cataaaagga
     2161 ttatcttcat ctagtacctt acataaggca tctaattttt ttctatctgt agtttctact
     2221 acttcttgtt ttatattatt aagagtgatt tcttcttttt gaatagaaac tacaacagga
     2281 ttttttgtgt atctataagc taattttttt acttgagaat ttatagttgc agaaaagcat
     2341 agtgtttgct ttttgtttga agtctctttt aaaatagctt ccacttcgtt tttaaatccc
     2401 ataagcaaca tttggtctgc ttcatctaaa acaaaagtct ttaacttact aaggtctatt
     2461 gtttttctat ttagatggtc taaaagccta cctggtgtag ctataatcat atgaatattt
     2521 ccttttagtt tctttatttg agaaccaata tcttttcctc catatgctgc aagaatactt
     2581 acatctttag attctttaag tttcatagct tcttctgtta tttgaatagc tagttctctt
     2641 gtaggagaaa gtataagtac ttgtatatca tttatatcta atgatatatt ttcaaatatc
     2701 ggaagtaaaa aagctagtgt ttttcctgtt ccagtttgag cttctgctat tacatctttt
     2761 ccttctttta ttagtttaat actttctttt tgtataggtg taggtgttgt tattcctgat
     2821 ttttttagaa tatttattgt attttcacta atccctaagc ttttgaaatc cattgttatc
     2881 ttacctttca tgaaaaattt tcttttttat taaccaatta ataatatcat atttgtaagt
     2941 atttttagta aa
//
LOCUS       DAMJWP010000306         2923 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.313, whole genome shotgun sequence.
ACCESSION   DAMJWP010000306 DAMJWP010000000
VERSION     DAMJWP010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.313"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            25..531
                     /locus_tag="PC700_003463"
     CDS             25..531
                     /locus_tag="PC700_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HDF2582728.1"
                     /translation="MKEYVVVDLETTGLDPYKGCEIIEIGITEIKNEQIVKNYSRLIK
                     PKGIISSFITELTHISNEMVENEEPLELVLPRFRKYIGDRTIIAHNAKFDLKFLNYYL
                     RMLDLEPINNYICTVELLKKCKSYKGKNKKLETACAYYNIENINAHRADSDTLATAKL
                     FLKIKDEY"
     gene            659..1675
                     /locus_tag="PC700_003464"
     CDS             659..1675
                     /locus_tag="PC700_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HDF2582729.1"
                     /translation="MGYYNDNDNNKIRRKKISNNKDSNKKSKHSYTSANKKNKSPNTS
                     SSKRMSEKVDSQNLIKRDRDSFTRDNVRPLNTDYNQRERRRVEYKQREKKLKSKKRKK
                     QKRIRIVRALLSIMLVALIIFSVAGAIFTVSAIKGSPEVTKKLIRSKYISSEVVSIKQ
                     MPNDLKHAIVSIEDERFYKHKGVDVISLVRSVLNNVFTDTTQGASTIDMQVSKNLLTS
                     NDKTMKRKIRDMYNAKQMNKIMTKDEILEAYLNNMYLGKNAYGAAKGAEVYFGKKVSE
                     LNLAECAMLAGMTNNPARYIDHGEAKKRQVIVLYKMHQLGYITDKEYRIAKADDTPFK
                     SEID"
     gene            2029..2733
                     /locus_tag="PC700_003465"
     CDS             2029..2733
                     /locus_tag="PC700_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HDF2582730.1"
                     /translation="MKKFVIEDAFWNLFPSAKIGVIICNDIDNSIKDEDYKKIILKGE
                     EEALKYLEDSEFSNNQVIKVWREAFKKFKTKKGARSSIEALLKRVHNGNNIGTINPLV
                     DIYNFISLKYALPCGGEDIDKFIGDIRLTRAIGNEEFIPLGTDENSFPYEGEIIYKDD
                     GGAICRCWNWREAVRTMLTEDTNNAFLCIELVDESRFKEFENALNELAKIVQEKLGGN
                     CKISILDIDNKEVSVD"
BASE COUNT         1229 a          276 c          520 g          898 t
ORIGIN      
        1 catttaataa taaggagaag agatatgaaa gaatatgtag ttgtggattt agaaactaca
       61 ggattagatc catataaagg ttgtgaaata atagaaatag gaataacaga aataaaaaat
      121 gaacaaattg taaaaaacta ttcaaggttg ataaaaccta aggggataat ttcttcattt
      181 ataacagaat tgacacatat aagtaatgaa atggtagaaa atgaagaacc actagaatta
      241 gttttaccta gatttagaaa atacataggt gatagaacta taattgcaca caatgcgaaa
      301 tttgatttaa aatttttaaa ttactatctt aggatgttag atttagaacc aataaacaat
      361 tatatatgta cagtagaatt attaaaaaaa tgtaaaagtt ataaaggaaa aaacaagaaa
      421 cttgaaacag cttgtgctta ttacaacata gagaatataa atgctcatag agctgacagt
      481 gatacattag caactgcaaa actatttttg aaaataaaag atgaatatta attataaata
      541 tagagaaaat tagtgaaaat atcctttata aaattgaccc ttaagcttac aattttgttc
      601 tttgatttat tttgtagaac aatgtataat gtaccttata tgaagaaggg agactaaaat
      661 gggttattac aatgataatg ataataataa aataagacgt aaaaaaatta gtaacaacaa
      721 agatagtaat aaaaaatcta aacattctta tacttcggca aataaaaaaa ataaaagtcc
      781 taatacaagt tccagtaaaa gaatgagtga aaaagtagat tcacagaatc ttataaaaag
      841 agatagagat agttttacaa gagataatgt aagaccttta aatacagatt acaatcaaag
      901 agaaagaaga agagtcgagt ataaacaaag agagaaaaaa ttaaaaagta aaaaaagaaa
      961 aaaacaaaaa aggattagaa tagttagggc attattatct attatgttag ttgcactaat
     1021 tatattttca gtggcaggtg caatatttac agtttcagct ataaaagggt caccagaagt
     1081 tactaaaaag ttaattaggt ctaaatatat aagtagtgag gttgtaagta taaaacaaat
     1141 gccaaatgat ttaaaacatg caatagtttc catagaagat gagagatttt ataagcataa
     1201 aggagtagat gttatttctc tagttaggtc agtattaaac aatgtgttta cagatacaac
     1261 acaaggtgca agtactatag atatgcaagt ttcaaaaaac ctgcttacta gtaatgataa
     1321 aactatgaag cggaaaataa gggatatgta taatgctaag cagatgaata agattatgac
     1381 taaggatgaa attcttgagg catatttaaa taatatgtat ctaggtaaaa atgcatatgg
     1441 agctgcaaag ggagctgaag tctactttgg taaaaaagtt agtgaattaa atttagctga
     1501 gtgtgctatg ttagctggta tgacaaataa tcctgcaagg tatattgacc atggagaggc
     1561 taagaagaga caagtgattg tactgtataa gatgcatcag cttggttata taactgataa
     1621 ggagtataga atagcaaaag ctgatgacac tccatttaaa tcagaaattg attaaaattg
     1681 taagaatata gtattttcaa tagtttaaac aaacaaaaat gtaataaaaa aggtgatttg
     1741 ccacccattt gccaccataa ttataatcta atggcaaatt gttttaatgt acaaactaga
     1801 taatagaata ttgttttaaa tgctatatta tgctaaaatt taattgtatt ttgtaaatta
     1861 aaaaacttat aatttagttt tctagggttc cgcattttat aatggtcagg tccaagagaa
     1921 aacgcgcaat tgttgtgtac acggaaggat aaaagcctgg gagatattta ataatatttc
     1981 tataggcttt tttattttta catattttaa atttgaggag gttttctaat gaaaaaattt
     2041 gttattgagg atgcattttg gaatctattt cctagtgcaa aaattggagt tattatttgt
     2101 aatgatatag ataactctat aaaagatgaa gactataaga aaataatttt gaagggagaa
     2161 gaagaagcat taaaatactt ggaggactca gaatttagca acaaccaagt gattaaagta
     2221 tggagagagg catttaaaaa atttaaaacg aaaaaaggag caaggtcttc tattgaagca
     2281 ttattaaaaa gagttcataa tggaaataat ataggaacta ttaacccttt agtggatatc
     2341 tacaatttta tctcattaaa atatgcatta ccttgtggag gggaggatat agataaattt
     2401 ataggagata ttaggctaac tagagcaatt ggtaatgagg agtttatccc actagggaca
     2461 gatgaaaatt catttccata tgaaggagaa ataatttata aagatgatgg tggagcaatt
     2521 tgtagatgtt ggaattggcg tgaagcagtg agaacaatgc ttactgaaga tactaacaat
     2581 gcctttttat gcattgaatt agtagatgaa agtagattta aagaatttga aaatgcttta
     2641 aatgaattag ctaaaatagt tcaagaaaaa ttgggtggga attgtaaaat ttcaatttta
     2701 gatattgata ataaagaggt atcagtggat taatctggag atgctgttgt aatggactat
     2761 tctagttata aagatactta tatttagggt aataagttct attttagatg taacgattat
     2821 caaaggatat aatatagaaa ataaaaatgt agaatataaa aagcctttaa aataagaaaa
     2881 aaatgtattt taaaggcttt tattatactc ttttttattt gca
//
LOCUS       DAMJWP010000307         2885 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.314, whole genome shotgun sequence.
ACCESSION   DAMJWP010000307 DAMJWP010000000
VERSION     DAMJWP010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.314"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            304..1581
                     /gene="hflX"
                     /locus_tag="PC700_003466"
     CDS             304..1581
                     /gene="hflX"
                     /locus_tag="PC700_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDF2582731.1"
                     /translation="MDMTKKGITVGLNINNKSEDFNELMIELENLCSACDIDVVGSIT
                     QNAKQVNRAFYIGTGKVEEILNLIKKENIEIVIFYNELSTSQLKNLEEKLNCEIIDRT
                     ALILDIFAQRAKTREAKLQVEVASLKYMLPRLIGSNENLGRQSGGVGTKNRGSGEKKL
                     ELDRRRIEEKITSLNRELYDLKFQRETQRSMRRKSNLPNVALVGYTNAGKSSIMNKLV
                     DIFKNSEEKKVFEKNMLFATLETSVRNIVLANNKEFLLSDTVGFVSNLPHDLVKAFRS
                     TLEEACEADVLLHVIDISNPSYKSHIKVTEDTLKQIGADGIPMIHVYNKIDLIDVEVL
                     DRILDSIDKEGIFVSVKKDINIDKMIKCICDSIFKDCVRCKFLIPYDKGHVVSYFNEN
                     TSIINTEYREDGAILDVECSNIEYNKYKKYALE"
     gene            1814..2311
                     /locus_tag="PC700_003467"
     CDS             1814..2311
                     /locus_tag="PC700_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2582732.1"
                     /translation="MDAESLMKKYMVDDDSRKQAIFASIFVMQNKLQTTCDKLDPELT
                     MKQWLLLAIADSVDETLTLTKLGELMGCSRQNVKKLAIALEKKGFIQIEQNEKDSRAI
                     CLVTKDRVQEYSKKVRGMQEKVLQLLFEDFTEKEVEQLFFGIMKLSIGIENVKNYVEN
                     NDVGE"
     gene            2361..2885
                     /locus_tag="PC700_003468"
     CDS             2361..2885
                     /locus_tag="PC700_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDF2582733.1"
                     /translation="MKSVVVIYKSKYGSTKKYAKWLSELLSCDIFETKNINIEKVMKY
                     DTIILGGGLYACNIAGISFLKKNYKDLQKKNLVVFGVGAAPYDDEVIKKLREVNFKDE
                     LNKVPCFYCRGAWDETKMSFIDYTLCSFFKKSIAKKERQGLKPAEAPLLSGEAFDWCN
                     KENLTPILNYIRKL"
BASE COUNT         1193 a          261 c          516 g          915 t
ORIGIN      
        1 aatgtatttt taatatatat ttagaatttt aatccttctt atgatataag tagcttgttt
       61 tgtattactt agatttgtaa ggagtttttt tattgaaaaa actgcataat atatttggga
      121 ggaggtgtaa gaaagtgatt ttttatataa gattgagaaa ataatgcacg attatggtta
      181 ttataatagt aaataagaag tgtcatttaa ggataagata aaaagcaaaa ttcttgatga
      241 atttaattat agagccataa tctcgtgcta tagattaaat aaaaaatagc agataaggag
      301 aatatggata tgacgaaaaa aggaataaca gtaggattaa acataaataa taaatctgag
      361 gactttaatg aactaatgat tgaacttgaa aacttatgtt cagcatgtga tattgatgtt
      421 gtagggagta taactcaaaa tgcaaagcag gttaatagag cattttacat tgggacaggt
      481 aaagttgagg aaatattaaa cttaataaag aaagaaaaca tagagatagt tatattttac
      541 aatgaacttt ctacatcaca actaaaaaat cttgaagaaa agcttaattg tgaaattata
      601 gatagaacgg cattgatact tgatatattt gcacaaaggg caaaaactag agaagctaaa
      661 cttcaagtag aagtagcaag tttaaaatat atgttaccaa gacttatagg ttcaaatgaa
      721 aatttaggac gtcaaagtgg tggagttggt actaaaaata gaggttctgg tgagaaaaaa
      781 ttagaacttg accgtagaag aatagaagaa aagataacat ctttaaatag ggagttgtat
      841 gatttaaaat tccaaagaga aactcaaaga agtatgagaa gaaaatcaaa tcttccaaat
      901 gtagctctag tcgggtatac aaatgctggt aaatcttcaa taatgaataa actagtagat
      961 atatttaaaa actctgaaga aaagaaagtg tttgagaaaa acatgttatt tgctacactt
     1021 gaaacttctg tcagaaatat agttcttgca aataacaaag aatttttatt atctgatact
     1081 gttggttttg taagtaatct accacatgac ttagtaaaag catttaggtc aacattagaa
     1141 gaagcatgtg aggcagacgt actgttacat gttattgata tttcaaatcc aagctataaa
     1201 agtcatatta aagtgacaga agatacttta aaacaaatag gtgcagatgg tatacctatg
     1261 atacatgtgt ataataaaat tgatttaata gatgtagaag tattggatag aattttagat
     1321 agtatcgata aagaaggtat atttgtttca gttaaaaaag atataaatat agataaaatg
     1381 ataaaatgta tttgtgatag catatttaaa gattgtgtaa gatgtaagtt cttaattcca
     1441 tatgataaag gtcatgtagt ttcttatttt aacgaaaata cgagcattat aaatacagaa
     1501 tacagagaag atggagctat tttagatgta gaatgtagca atatagaata taataagtat
     1561 aaaaagtatg ctttagagta aattttgaaa aacttattat agcctaaaat atatttaatt
     1621 tataatttat tataaattga ttgtaaatat gattttatga aacggtatag ccataatgct
     1681 atgccgtttt gccatttata ataaaaataa atttattgac acaatagtaa ctaattacta
     1741 taatataaag taactagtta ctatttaaaa ttatcaaaaa attaaaatct aagataaaga
     1801 gaagtgtata ataatggatg cagaaagttt aatgaaaaaa tatatggtag atgatgatag
     1861 tagaaaacaa gctatatttg caagtatatt tgtaatgcaa aataaacttc aaactacatg
     1921 tgataaatta gacccagaat taacaatgaa acaatggtta cttttagcta ttgcggactc
     1981 tgtagatgaa actcttacat taaccaaact tggagaatta atggggtgct caagacaaaa
     2041 tgtaaaaaaa ctagctattg ctttagaaaa gaaaggtttt atacaaattg agcaaaatga
     2101 aaaagattca agagctatct gtcttgtgac aaaagataga gtgcaagagt attctaaaaa
     2161 agtaagaggt atgcaggaaa aagtattaca gcttttattt gaagatttta cagagaaaga
     2221 agtggaacag ttattttttg gaattatgaa attaagtata ggaattgaaa atgtgaaaaa
     2281 ttatgtagaa aataatgacg ttggagaata agcaagtatc aaatcaactt tgtatgacat
     2341 cagagagggg ggtaatttat atgaaaagtg tagtagtgat ttataagagt aagtatggtt
     2401 caacaaaaaa atatgctaaa tggctttcag aattattgtc ttgtgatatt tttgaaacta
     2461 agaatattaa tatagaaaag gttatgaagt atgatacgat tattttagga ggaggtcttt
     2521 atgcttgtaa tatagcggga atttctttct taaaaaaaaa ttataaagat ttgcagaaaa
     2581 aaaatttagt agtatttggc gtaggagctg ctccatatga tgatgaagta ataaagaaat
     2641 taagagaagt aaactttaag gatgaattaa acaaagttcc ttgcttttat tgtcgtgggg
     2701 catgggatga gacaaaaatg tcttttatag attatactct ttgtagtttt tttaaaaaat
     2761 ctatagctaa aaaagaacgt caaggtctta aaccagcaga agcaccttta ttgtctggag
     2821 aagcctttga ttggtgtaat aaagaaaatt taactccaat acttaattat ataagaaaac
     2881 tttaa
//
LOCUS       DAMJWP010000308         2870 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.315, whole genome shotgun sequence.
ACCESSION   DAMJWP010000308 DAMJWP010000000
VERSION     DAMJWP010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.315"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            387..881
                     /locus_tag="PC700_003469"
     CDS             387..881
                     /locus_tag="PC700_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF2582734.1"
                     /translation="MKNKKGFTLVELLVVIAIIGILAIIALPALFKNIEKAKIAKLEA
                     DISAIKSASLSYYADESKYTDGGMISWVKKDGKIIINGGFKDDPLADKIENLGMPYNG
                     SYLLMSSPGHEKYLELSILPEGEISKSGLDKLKNDYGNLIDITNDQNKINIVIKLLNN
                     KSNT"
     gene            1278..1428
                     /locus_tag="PC700_003470"
                     /pseudo
     CDS             1278..1428
                     /locus_tag="PC700_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897489.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1904..2092)
                     /locus_tag="PC700_003471"
     CDS             complement(1904..2092)
                     /locus_tag="PC700_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2582735.1"
                     /translation="MTKYDFNFKLKVAKFYLNREGRDILIVKQYGVYDHSQVERWVNI
                     LGEGALKIKKFSILYGSS"
     gene            2254..2604
                     /locus_tag="PC700_003472"
     CDS             2254..2604
                     /locus_tag="PC700_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HDF2582736.1"
                     /translation="MIQKKIFMGFCGFLGFLSLRYFSSGNVTDLTYIGFFAFFSNFII
                     AKINGDKADERYVQDEKAAMAFTGQLAIIELFILWCITIVSRNVELMCVLLSITYAIT
                     LNVYAIKLYILEEK"
     gene            2606..2806
                     /locus_tag="PC700_003473"
     CDS             2606..2806
                     /locus_tag="PC700_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2582737.1"
                     /translation="MPKFICNLKKYRQLKELTQEQLAEKVGVRRETIMRLEAGKYNPS
                     LKLAIDISKIVDTPIDELFIFD"
BASE COUNT         1059 a          332 c          363 g         1116 t
ORIGIN      
        1 aattattttg attttataga tacacagtta gatgatttta taatagagca taaaaaaaat
       61 gtagtataat cttgtttata tcaagttatg gtattggtaa gtaagtataa ttaaatagac
      121 attattttct gggtcctctt tttattttca tctgctttta aataatatta ttgacattga
      181 tatgactgta gttttcttta ttttaatcca ttgcttatat cttttctaac aagaaatgtc
      241 attttaaatc atttctataa attgtaaaat aatagtatat ttgtcataga aagtgatttt
      301 tttgtctata tatggtaaaa tatagaaagt agaaatcaac aaaataaaaa tttatatatt
      361 attaatttta tatggagggg aaataaatga aaaataaaaa aggatttact ctagtggaat
      421 tattagtagt aattgctata ataggaatat tggcaataat agcacttcca gcattattta
      481 aaaatataga aaaagcaaag atagctaaac ttgaagctga tataagtgca ataaaaagtg
      541 catctcttag ttactatgct gatgaatcca agtatactga tggaggaatg atatcatggg
      601 taaagaaaga tggaaaaata ataataaatg ggggttttaa agatgaccca ttagcagata
      661 aaatagaaaa tttagggatg ccttataatg gttcatatct gttaatgtca tctcctggtc
      721 atgaaaaata tctagaatta agcatacttc cagaaggaga aataagcaaa agtggtctag
      781 ataaattaaa aaatgattat ggaaatttaa tagacataac gaacgatcaa aataaaataa
      841 atattgtaat aaaactttta aataataaat cgaatactta aatacaataa atttgattta
      901 ataattactg atgaaataaa caatcttgtt tggagaaatg aaaaaagcac tccccaagaa
      961 tagaatgctt agttataact acagaagtgt attggcatac acttctattt tttattatat
     1021 cacataattt atgttgctaa aaatacctat ttaagttgtt tttaaattgt gttgagtaat
     1081 attcttgata gtttatacat taatgaattt caaaaaaata agcactcttt ataataaaaa
     1141 gtactttagt gtatgttaat gtcctatcta ataaaattgt aacatgtctt atgctttagt
     1201 atgataattt tcattctctt tattgttgca acttatacta tagttttgct aatttatctg
     1261 caaacacaac ttctttattg catgtgtatc aaccaaaaac atcagaatac ttacttaata
     1321 catctgcaaa atcgtatctt tcatctatag tattcttatt tgacaatata acaaaaaatg
     1381 aatcgagata tgcctttttt ataattagat atattttata aattctagca tatcatttgt
     1441 tattttctga tatgctattg atgttttaaa tcttctattt attttattat tttttacttt
     1501 tccaattgta cctttatagg agttatattt atgtgattta tgaaaaaatg ctatgcattt
     1561 aagttctaat tttttgataa tacatctaac tttcttttga ttaataatga taccactatt
     1621 tttaatgtgg cttccctgag gcacacatct tttttaaaaa ctcaagctca tcttttaaaa
     1681 atttattttc atattctaat tcttgttctc ttgttaattt attttttctt tagtgttttt
     1741 gtttttattc attatggtct tcctttcatt ttgaaaaagg catcaacacc actatgtaaa
     1801 aatttttgtt ttcatgttgc tataactgtg ctattgttta ttccaaaata cttagcgatt
     1861 tattgtacac tatacttagt tatcatcata tagtttataa catctaactt gaaccatata
     1921 gtatagaaaa cttttttatt tttagtgcac cctctcctaa tatattaacc catctttcaa
     1981 cttgagaatg gtcataaact ccatattgtt ttactattaa aatatctctt ccttctctat
     2041 tcaaataaaa tttagctact tttaatttga aattaaaatc atatttagtc atacataaac
     2101 atctcaagcg ttagattttt actctaacaa ttatggtgca gtacaaatat tgtataatac
     2161 tgattttcta ttgacaaaag aatataaaag agatatattt gtattataaa gatacaaata
     2221 tatctctttt aaaattacta aggaggataa aaaatgattc aaaaaaaaat ttttatgggt
     2281 ttttgtggtt tccttggatt tttaagtctt agatattttt catctggcaa tgttactgat
     2341 cttacatata ttggattttt tgcttttttc tcaaacttta ttatagcaaa aataaatggt
     2401 gataaagcag atgaaaggta tgtacaagat gaaaaagcag ctatggcatt tacaggacag
     2461 cttgctatta tagaattatt cattttatgg tgtattacta tagtgtctag gaatgtagaa
     2521 ttaatgtgtg ttttactatc aattacatat gcaatcactt taaatgttta tgctataaaa
     2581 ctttatattt tagaggaaaa ataatatgcc caaattcatt tgtaacctaa aaaaatatag
     2641 acaattaaaa gaattaactc aagaacaatt agcagaaaaa gtaggagtta gaagagaaac
     2701 cattatgcgt ttagaagcag gaaagtataa tccttctttg aaattagcaa tagatatatc
     2761 aaaaatagta gatacaccta ttgatgaatt gtttattttt gattagtttt tgcattatta
     2821 aaaatgtgtt ttaaatacaa acttaaattc aatctttaag tcactcctga
//
LOCUS       DAMJWP010000309         2858 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.316, whole genome shotgun sequence.
ACCESSION   DAMJWP010000309 DAMJWP010000000
VERSION     DAMJWP010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.316"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            49..2406
                     /locus_tag="PC700_003474"
     CDS             49..2406
                     /locus_tag="PC700_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HDF2582738.1"
                     /translation="MDKVNLERYSCDINVGLNKNQLQSRIDDNLINAFDNGKTKTYKQ
                     IFKDNIFTLFNLINIVLVCLLVSVGSFKNTLFIFIAVINTIIGVIQEIRAKRLLDNLS
                     VIVSNKVSVIREKEKKEIDVQELVLDDIMILKTGNQICADSKVLNGKLEVNESLVTGE
                     SDIIIKNKDDFLYSGSFVVSGEAFVRVENIGKDNYANKIVNDAKISKKHNSQLRNSLN
                     SILKIVGIIIIPLGIILFAKQHFGNGDSIATSVVGTVAAVNGMIPEGLTLLTSIALAV
                     GTIKLASHKTLVQELYCVETLARVDTLCLDKTGTITEGKMQVDDIVMLEEVDINEIMG
                     NICNSLKDDNATLNAIRDKYNVLDTYKAKNLIPFSSERKYSGVTFENKGTYLLGAFEF
                     IFKEKNKKIRIEVEKYSKKGNRVIVLAHSDSYMEDNNIPEDIKPLAFILILDKIRDEA
                     KETLEFFYNEGVDIKIISGDNPITVSEVARKAGLKTASNFIDATTLKNDNDIYEAVDK
                     YSVFGRVSPQQKKQMILALKKQNHTVAMTGDGVNDVLALKEADCSIAMASGSDVTKNV
                     SNLVLLDSNFASMPKVVMEGRKVINNIQRASSLFLVKTIFSFFLSLLTLFFFSRQYPF
                     VPIQLSLIGAVTVGIPSFFLALEANKSRISGNFLLNILKNALPGALCVTVNVIIVYNI
                     VEYLGYSSSVFSTMCAILTGVCGLLILRQQCLPFNKERLFLIVSMTIAFVIGILFLGG
                     LFSFVALSRELILITMALAILMPIMIKVMLFVLDEILETIVPDLN"
     gene            complement(2492..2809)
                     /locus_tag="PC700_003475"
     CDS             complement(2492..2809)
                     /locus_tag="PC700_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HDF2582739.1"
                     /translation="MSEFKNVTAVKKANVYFDGKVSSRVIILPNGERKTLGLMLPGEY
                     TFSTREEEIMEMLAGSMDVKLPGSNEFVTYKEGQKFNVPSDSSFDLKVNEVVDYCCSY
                     IAD"
BASE COUNT         1073 a          327 c          465 g          993 t
ORIGIN      
        1 atttgtagat ttgagtataa aaaagtgcta agttgaaagg agtaaaatat ggataaggtt
       61 aatttagaaa ggtatagttg tgatataaat gttggtttaa ataaaaatca acttcaaagt
      121 agaatagatg ataatttaat taatgcattt gataatggaa aaaccaaaac atataaacaa
      181 atttttaaag acaatatatt tacacttttt aatctcataa atattgtttt agtctgttta
      241 ttagtttcag ttggttcttt taaaaatacc ttatttatat ttattgctgt aattaatact
      301 attataggag taatacaaga gataagagct aaaagattat tggacaatct atctgttata
      361 gtttcaaata aggtatctgt cataagagaa aaagagaaaa aagagattga tgtacaagaa
      421 ttagtattag atgatattat gattttaaaa acaggtaatc aaatttgtgc agattcaaaa
      481 gttttaaatg gaaaattaga ggtaaatgag tctttggtta caggtgaatc tgatattatt
      541 ataaaaaata aagacgattt tttatattct ggaagttttg tagtttctgg agaagctttt
      601 gtaagagttg aaaatatagg aaaagataac tatgcaaata agatagttaa tgatgcaaag
      661 atttctaaaa aacataattc tcaacttaga aattcattaa attcaatatt aaaaattgta
      721 ggtataataa taattccttt aggaattata ttgtttgcaa aacagcattt tggaaatgga
      781 gattctatag ctacatctgt agtaggtaca gtggcagctg taaatggtat gataccagag
      841 ggattaacat tacttacgag cattgctcta gctgtaggaa ctataaaact tgctagtcat
      901 aaaactttag tgcaagaact gtactgtgta gagactcttg ctagagttga tacactatgt
      961 cttgataaga caggtacaat tacagaaggt aaaatgcaag ttgatgatat agtaatgttg
     1021 gaagaagtgg atattaatga aataatgggg aatatatgta attctctcaa agatgataat
     1081 gcaacactaa atgcaataag agataagtat aatgttttag atacgtataa agctaagaat
     1141 ttaataccat tttcttcaga aagaaaatac agtggggtta cctttgaaaa taaaggtaca
     1201 tatttattag gtgcatttga gtttatattt aaggaaaaaa ataaaaaaat tagaatagaa
     1261 gttgaaaaat attctaaaaa aggaaacaga gtaatagttt tagctcatag tgattcttat
     1321 atggaagata ataatattcc tgaagatata aagccactag cttttatatt gatattagat
     1381 aaaattagag atgaagctaa ggaaacttta gaattttttt acaatgaagg tgtagatata
     1441 aaaattatat ctggtgacaa tcctataaca gtaagtgagg tagctagaaa agcaggtctt
     1501 aaaacggcta gtaattttat tgatgcaaca acacttaaaa atgataacga tatatacgaa
     1561 gcagttgata aatacagtgt ttttggaagg gtaagcccac aacagaaaaa acaaatgatt
     1621 ttagcattaa aaaaacaaaa tcatacagta gctatgactg gagatggtgt aaatgatgtg
     1681 ctagcgctta aagaagcaga ttgcagcatt gccatggcat ctggaagtga tgttactaag
     1741 aatgtttcta atttagtctt attagattca aactttgcat caatgccaaa ggttgttatg
     1801 gaaggacgaa aggtaattaa taacattcaa agagcatctt ctttgttttt agtaaagaca
     1861 attttttcat tcttcttgtc actattaaca ttattcttct tcagtagaca gtatccattt
     1921 gttcctatac aattatcact aattggagct gtaacagtgg gtataccatc attcttctta
     1981 gctcttgaag caaacaaaag tagaatttca ggaaactttt tactcaatat attaaaaaat
     2041 gctttaccag gagcactatg tgtgacagta aatgtaatta ttgtttacaa tatagttgaa
     2101 tatttaggct atagttcaag tgtattttct acaatgtgtg ctatattaac tggtgtatgt
     2161 ggtcttttaa tattaagaca acaatgtttg ccatttaata aagaaagatt atttttaatt
     2221 gtaagtatga ctattgcatt tgttattggg attctatttt taggtggatt attctcattt
     2281 gtagcattga gtagagaatt aattctcata actatggcat tagcaatatt aatgccaatt
     2341 atgataaaag ttatgttatt tgtcttggat gagatattag agacaattgt tccagattta
     2401 aattaatttt aaaatgatat aaataagagc agttttagtt gaactgctct ttttagatgt
     2461 gtttaatttt atataagaat atgtattttt attaatcagc tatatatgag caacagtagt
     2521 caacaacttc attaaccttt aaatcaaaac ttgaatcact aggaacatta aatttttgcc
     2581 cttctttata agttacaaac tcattgcttc caggcagttt tacatccata cttccagcaa
     2641 gcatctccat tatttcttct tctctagtag aaaaagtata ttctcctggc aacattaatc
     2701 ctaaagtttt tctttctcca ttaggcagta ttattactct gctacttacc ttaccatcaa
     2761 aataaacgtt tgccttcttt actgcagtaa cattcttaaa ttcactcatt agataaaacc
     2821 tccaaaatta tttgtacata tatattatat aaaaataa
//
LOCUS       DAMJWP010000310         2805 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.317, whole genome shotgun sequence.
ACCESSION   DAMJWP010000310 DAMJWP010000000
VERSION     DAMJWP010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.317"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            158..1081
                     /locus_tag="PC700_003476"
     CDS             158..1081
                     /locus_tag="PC700_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HDF2582740.1"
                     /translation="MGSGKVGTSLANYFLSKGYCVSGFYGRNQLSLLESINLTKTKIY
                     SNLRDIIYENDILFITTSDDSIEIIDKKLSKFNLKHKYICHTSGSLKSSILFCSKKAG
                     ALIYSIHPIFAFSNKNTNIKKMKDICFSIEGDNEQDDLVIQNFIDGLGNQFFIRDKDT
                     SSTYHLANVLVSNLVLSLLDIGVSYFIKLGLSEEESLKAISPLVKKNIENILDNGFTK
                     ALTGPVSRGDITPIRKHLSVLNKKDEDIYKILSLNLLKIIALGNDNSLKGTKNITIEN
                     AIENLISKSEKYKEIYKLLGGKNDEKYHTDF"
     gene            1056..1883
                     /gene="panB"
                     /locus_tag="PC700_003477"
     CDS             1056..1883
                     /gene="panB"
                     /locus_tag="PC700_003477"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HDF2582741.1"
                     /translation="MKNTIQTFKNAKYEGKKLSMLTAYDYSIAKIMDECDINGILIGD
                     SLGMVIKGEENTLSVTIDEIIYHTKAVKNGAKNALIVSDMPFLSYHVSIEDAVKNAGR
                     LIKEGGAHAVKLEGGSNVIKQIESIVNAQIPVMGHLGLTPQSVNSFGGFKVQGNTSET
                     ARQLIEDAKLIEKAGAFSIVLEGVPTKIADMVTNSISIPTIGIGAGINCDGQILVYQD
                     MLGVFGDFVPKFVKQYANIGDIMKDSIKNYILEVNTGAFPQEKHSFSINESELEKLY"
     gene            1902..2750
                     /locus_tag="PC700_003478"
     CDS             1902..2750
                     /locus_tag="PC700_003478"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HDF2582742.1"
                     /translation="MLVKEIKLLRNIIKDWRKHGYSIGLVTTMGFLHEGHQSLIKKAV
                     KENDKVVVSVFVNPTQFGPNEDFNSYPRDIDKDFKYCMDSGATVVFNPSPEEMYLKGN
                     CTTINVSGLTDFLCGAKRPVHFGGVCLVVSKFLNIVTPDKAYFGEKDAQQLAVIKRMV
                     KDLNINTEIIGCPIIRENDGLAKSSRNTYLSEEERKSALILNKSLSLAKEELVKGNLN
                     PKNIKELITAKINSEHLAKIDYVEIVDSETLQPVKQIEHSILVAIAVFIGKTRLIDNF
                     TFELNI"
BASE COUNT         1122 a          339 c          459 g          885 t
ORIGIN      
        1 aaaatggtaa gatatttatg gatggtatct tgttataaga actatacttt aaatgttttt
       61 aattattttg tcttatgtaa ctcatatcct caggaatgag attagaaatt aagatgttgt
      121 atcacaaagt ggaggtgata ctattaaaat aggctttatt ggttctggaa aagttggaac
      181 atctttggca aattattttt tatcaaaagg ttattgtgtt tcaggctttt atggaaggaa
      241 tcaattatct ttattagaat caataaattt aacaaaaaca aagatttaca gcaatttgag
      301 agatataatt tatgaaaacg atatattatt tattacaaca tcagatgatt ctattgaaat
      361 tatagacaaa aaactttcca aatttaattt aaaacataaa tacatttgcc acacaagtgg
      421 ctctttaaaa tctagtatat tgttctgttc aaaaaaagca ggtgcattaa tttattctat
      481 ccatcctata tttgcatttt ccaataaaaa taccaacata aaaaaaatga aagacatttg
      541 tttttccata gaaggtgata atgaacaaga tgatttagtt attcaaaatt tcatagatgg
      601 tttgggaaac caatttttta taagagataa ggatacttca tccacttatc atctagctaa
      661 tgttttagtt tcaaatttag tattatcttt actagatata ggagtaagtt actttattaa
      721 attaggttta tctgaagagg aatctttaaa agctatcagt cctttagtaa aaaaaaatat
      781 agaaaatata cttgataatg gctttactaa agctcttact ggtcctgttt ctcgtggaga
      841 tataacacca ataaggaaac atctatctgt attgaataaa aaagatgagg atatctacaa
      901 aatactatcc ttaaacctac taaaaataat tgccttagga aatgacaatt cattaaaagg
      961 tacaaaaaat attactatag aaaatgcaat cgaaaattta ataagtaaat ctgaaaaata
     1021 caaagaaatt tacaaacttt taggaggaaa aaacgatgaa aaataccata cagactttta
     1081 agaacgctaa atatgaagga aaaaaactat ctatgcttac tgcttatgac tactcaattg
     1141 caaaaataat ggatgaatgt gatataaatg gtattttaat aggcgattca ttaggaatgg
     1201 tcataaaagg tgaagaaaac actctatctg ttactataga cgaaattata tatcatacaa
     1261 aagctgtaaa aaatggtgca aaaaatgcct taatcgtaag tgatatgcct tttctatctt
     1321 accatgtttc tatagaagat gcagttaaaa acgctggtag acttataaaa gaaggaggag
     1381 ctcatgctgt aaagttagaa ggtggctcaa acgttataaa gcaaatagaa agcattgtaa
     1441 atgctcagat acctgtaatg gggcatttag gattaactcc tcaatcagta aattcatttg
     1501 gtgggtttaa ggttcaagga aataccagtg aaacagcaag acaacttatt gaagatgcta
     1561 aattaataga aaaagcaggt gcattctcta ttgtccttga aggagttcct acgaaaatag
     1621 ctgatatggt aactaattct atttcaattc ctacaatagg tataggggca ggaattaact
     1681 gcgatggaca aattttagtt tatcaagata tgttgggagt gtttggagat tttgttccta
     1741 agtttgtaaa gcagtatgca aatataggag atataatgaa agattcaatt aaaaattaca
     1801 tcctagaagt taatacaggt gcatttccac aagaaaaaca cagcttttca ataaatgaat
     1861 ctgagttaga aaaattatac taatattaag gatggtacaa aatgcttgta aaagaaatta
     1921 aattactaag aaatataatc aaagattgga gaaaacatgg ctattctata gggcttgtta
     1981 caacaatggg atttttacat gaaggacacc aaagtcttat aaaaaaagct gtaaaagaaa
     2041 atgataaagt agtagtgagt gtatttgtaa atccaactca atttggacca aatgaagatt
     2101 ttaatagcta tccaagagat atagataaag attttaaata ttgtatggat agtggagcaa
     2161 cagtagtctt taacccatca ccagaagaaa tgtatttaaa aggtaattgt actaccatta
     2221 atgtttctgg acttacagat tttctttgtg gagctaaaag acctgttcat tttggaggtg
     2281 tctgcctagt tgtttcaaag tttctaaata tagtaacacc tgataaagct tattttggag
     2341 agaaggatgc ccaacaattg gctgtaatta aaagaatggt caaggattta aacataaata
     2401 cagaaataat aggctgtcct ataattcgtg aaaatgatgg attagcaaaa agttcgagaa
     2461 atacatatct ctctgaggaa gaacgaaaat ctgctctaat attaaataag agcttaagtt
     2521 tagcaaaaga agaattagta aaaggcaatc taaatccaaa gaatataaaa gaattaataa
     2581 cagctaaaat aaattctgaa cacttagcaa aaatagacta tgttgaaatt gtagatagtg
     2641 agacattaca acctgttaag caaatagaac attctatcct tgtggcaatt gctgtgttta
     2701 taggaaaaac cagactaatt gataatttta catttgagtt aaatatatag tatataattt
     2761 tatataatat atctaaaaaa taatagaata cctaaaaagt aataa
//
LOCUS       DAMJWP010000311         2791 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.318, whole genome shotgun sequence.
ACCESSION   DAMJWP010000311 DAMJWP010000000
VERSION     DAMJWP010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.318"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..330
                     /locus_tag="PC700_003479"
     CDS             <1..330
                     /locus_tag="PC700_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2582743.1"
                     /translation="DIICIEDLQVKNMIKNRRLSRLISDVSWSEFVRQLEYKANWYGR
                     KIVKVGKFFASSQICNKCGYKNEEVKDLNIREWICPSCNETHNRDINASINILKEGLR
                     LITIQNK"
     gene            395..637
                     /locus_tag="PC700_003480"
     CDS             395..637
                     /locus_tag="PC700_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDF2582744.1"
                     /translation="MQNSMKQEPCDFSHGRFRFKTSDNRVLIPVVIFLGSIVTALCDL
                     ISRVILSPVELPISTITSLLGAPIVVMLLVKRKDRA"
     gene            640..1836
                     /locus_tag="PC700_003481"
     CDS             640..1836
                     /locus_tag="PC700_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2582745.1"
                     /translation="MLKTNNLSVGYNNKVVISNINVEVKNGEILCLLGSNGAGKTTLL
                     RSLSKLISPIKGEIYLNGVNINCISRKALSKKMALVLTNRLLGDLMTVQDIVNIGRYP
                     YTGFFGSLSKKDLIMVDEALESVDALHLKKRYFDELSDGEKQKVLVARALVQEPEIII
                     LDEPTTHLDIKHRLELINILKKLSKEKSISVILSLHEIDIALKSCDKVALIKNNKVIA
                     YGQPEDVVDEDIINSLYELDDKNFNSLLGSVEISNKSKNEVFIIGGGGKATPIYRAFT
                     KKGIGLYSGIIHENDIDYEIGRTMGIKMFTENPFEPISDESFDLAIRNLNDSKIIIDT
                     GFSVGETNKRNIDIIKEALKLDKKVYSFRNRDESKKYYDSLDSKIEHIDKVSQIINSA
                     DINNLL"
     gene            complement(1956..2681)
                     /locus_tag="PC700_003482"
     CDS             complement(1956..2681)
                     /locus_tag="PC700_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDF2582746.1"
                     /translation="MKTISKQELEKTLEKHYLWLKNNNAGEKADLKELRLENMDLRGY
                     CLSNIDFSWSDLINLNLEKADLENSIFNNSYLSDCSLKNSILKNADLSGANFRFCDLS
                     NSDIRGANLENSNLEHATLNGVISDESTPFFKLYCPETGAFIGYKKCFNFRMVQLLIP
                     SDAKRVSATSNACRCDKAKVLSITSIDGKESFKSARAYADENFIYRVGETIVAEDFNE
                     NRWKESTTGIHFFLTREEAIGYL"
BASE COUNT         1076 a          319 c          453 g          943 t
ORIGIN      
        1 gatattattt gcatagaaga tttacaagta aagaatatga ttaaaaatcg tagactatct
       61 cgcttaattt ctgatgtatc atggtcagaa tttgttcgtc aattagaata caaggctaat
      121 tggtatggta gaaaaattgt aaaggtaggt aaattctttg caagttcaca aatatgcaat
      181 aaatgtgggt ataaaaacga ggaagtgaag gacttaaata taagagaatg gatttgtcct
      241 agttgtaatg aaactcacaa tagagatata aatgcaagta taaatatatt aaaagaagga
      301 ttaagactaa taacaattca aaataaataa cttatgtaag aaccgtagga actatgggga
      361 tggcctgtgg agaattagta agacatattt tagtatgcaa aattctatga agcaggaacc
      421 ctgtgacttt agtcatggga ggttcagatt taaaacatca gataatagag ttttgatacc
      481 agtagtaata tttttaggca gtatagttac agctctttgt gatttaatat caagagtaat
      541 tttatcacca gtagaattac caataagcac gataacatct ttattaggag ctccaattgt
      601 agtaatgtta ttggtgaaaa ggaaggatag ggcttagaca tgttgaaaac taataattta
      661 agtgtaggat acaataacaa ggtagttata agtaatataa atgtagaagt aaaaaatgga
      721 gaaatactat gtcttttggg aagtaatgga gctggaaaaa ctaccctatt aaggtcctta
      781 tctaaactta tatcaccaat aaaaggtgaa atatatttga atggtgtaaa cataaattgt
      841 atatcaagaa aagcattatc aaaaaaaatg gctttagtac ttacaaatag attattaggt
      901 gatttaatga ctgtacaaga tatagtcaat ataggaaggt atccatatac aggatttttt
      961 gggagtctat ctaagaaaga tttaataatg gtagatgaag cattggagtc tgtagatgcc
     1021 ttacatttaa agaaaagata ttttgatgaa ctaagtgatg gagaaaaaca aaaggtgttg
     1081 gttgcaagag ctcttgttca agaaccagaa ataattatat tagatgagcc tacaactcac
     1141 ttggatataa aacatagatt ggagctcata aacatactga aaaaattaag taaagaaaag
     1201 tcaatatcag taatactttc attgcatgaa atagacatag cattaaaaag ttgtgataaa
     1261 gttgctctta taaaaaataa taaagtcata gcttatggtc agcctgaaga tgtagtagat
     1321 gaggatatta taaattcgct ttatgaattg gatgataaaa actttaatag tcttttaggt
     1381 tcagtagaaa ttagtaataa atctaaaaat gaagttttta taattggtgg tgggggtaaa
     1441 gcaacaccaa tttatagagc ttttactaaa aaaggtatag gtctgtattc tggaataata
     1501 catgaaaatg atattgatta tgaaattggt agaactatgg ggataaagat gtttacagaa
     1561 aatccatttg agccgataag tgatgagagt ttcgatttgg ctattagaaa tttaaatgat
     1621 tccaagatta taatagatac aggatttagt gtaggagaga caaataagag gaatatagat
     1681 attataaaag aggctttaaa actggataaa aaagtttatt cttttagaaa tagagatgag
     1741 agtaagaagt attatgacag tttggatagt aagatagaac atatagataa agtttctcaa
     1801 ataatcaata gtgcagatat aaataattta ttataattta tgaaataaaa tgtacttatg
     1861 tgctatagaa aaaagagtat agattataat ttttttgaaa ttacaatcta tactcttttg
     1921 ttattttgtt tatatattta taaaacttta gcattttata gataaccaat agcttcctct
     1981 ctagttaaaa aaaagtgaat ccctgtagtc gattctttcc atctattttc attaaaatct
     2041 tcagctacaa tcgtttctcc tactctatat ataaaatttt catcagcata tgcacgagca
     2101 cttttaaaag attctttacc atctatacta gtaattgaca gtacttttgc cttatcacat
     2161 ctacaagcat ttgatgtagc agacactctt tttgcgtcgc tgggtattaa taattgtacc
     2221 attctgaaat taaaacattt tttatagcct ataaatgctc ctgtttcagg acaatacaat
     2281 ttaaaaaaag gggtactttc atctgatatt actccattta aagtagcatg ttctaaattg
     2341 gaattttcta aattagctcc tctaatatct gagttagata agtcacaaaa tctaaaattt
     2401 gcaccactta agtctgcatt ttttaatata gaatttttta atgaacaatc cgaaagataa
     2461 gaattattaa aaatagagtt ttctaaatct gctttttcta aatttaaatt gataaggtca
     2521 gaccacgaaa agtcaatatt acttaagcag tatcctctta agtccatatt ttctaatctt
     2581 aattctttta aatctgcctt ttctccagca ttattgtttt taagccataa ataatgtttc
     2641 tcaagtgttt tttctaattc ttgtttagaa atagttttca ttaaaatcac cttcataaat
     2701 ttatttttat atatgtagta gcaaaatagt catatattta attatataac aataaataaa
     2761 aaacttctaa ataatatata aaatatcatc t
//
LOCUS       DAMJWP010000312         2775 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.319, whole genome shotgun sequence.
ACCESSION   DAMJWP010000312 DAMJWP010000000
VERSION     DAMJWP010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.319"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            84..524
                     /locus_tag="PC700_003483"
     CDS             84..524
                     /locus_tag="PC700_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2582747.1"
                     /translation="MSDKELSILLLESMNNFHNLIKIINNERYKKDKVLTERQFFALV
                     KIRKHDKIELKNLSRDLHVSTSSLCILLNKLVEQEYVYREEDSRDRRNTFYGITKNGE
                     KILDNEILKFVSIISDKMDCLDIDNKDKLFTSLEESKNIIEQLF"
     gene            540..1991
                     /locus_tag="PC700_003484"
     CDS             540..1991
                     /locus_tag="PC700_003484"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HDF2582748.1"
                     /translation="MNIKYSKEILKSLENAITVVALFEGEDVSTTFDKINMISRNFNS
                     KENSIDTVTLVENNNLVNVCLVGFGKRELLNREKIRIIGGNLCKKLKNILKNEAYKSS
                     DVNVLNLNLNSDEIGSFVEGILLGNYKFDKYKTKSKDFERKEVDTITIFTEKEVECQI
                     EKAKILANSTIIARNLVNEPSNVIYPKTLAIETVKLGAEFGFNVDVYEEEKIKALGMD
                     AFFAVSRGSINKPRFIVMRYFGDKESTDILGLVGKGLTYDTGGYSLKSNASMLDMKTD
                     MAGAASVIGAMCAISQSKLKKNVIAVVAACENALSGGSYKPGDIISSMAKKTIEVLNT
                     DAEGRLTLADAIYYIINNEKVTKVVDVATLTGAALTLLGNVATPIVTNNDDFYCELEK
                     AATLSGERVWKMPIYDEFKDMIKGEEADLKNTGGKNAGCITAGAFIGEFVGNTPWIHM
                     DIAGTSTSSKSIGYKAKGATGEPVRTLYYLAEI"
     gene            complement(2082..2750)
                     /locus_tag="PC700_003485"
     CDS             complement(2082..2750)
                     /locus_tag="PC700_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HDF2582749.1"
                     /translation="MFKIFLFSSEQFVSLFIFGLFLYYCPKLTKNILPYSYTVEKIIC
                     TLLVIIMALEQLLLISSGNYSTLNSLPIGINYICIYLCIAILIFKQYHLFNIFFSWSL
                     VCSVGELIFSKNLGYEFPSLIYFIFIFSKCLIIYADIYMVDVRKFRVNRYALRDNLAI
                     CFIYFSFIFLLNTFTNSQYYYGFLSHSTTAIFTFIFVTSIMYIPALLFNRDTFILEKK
                     KKSK"
BASE COUNT         1145 a          267 c          484 g          879 t
ORIGIN      
        1 aaaagaagta ttatattata gatatataaa cgcatttgag atataagagg gagttataaa
       61 tcattagttt tgaggtgaat gttatgagcg ataaagaatt atctattttg ttgttagaaa
      121 gtatgaataa ttttcataat ttaataaaga ttattaataa cgaaagatat aaaaaagata
      181 aggttcttac agaaagacag ttctttgcct tagtaaaaat aagaaaacat gataaaatag
      241 agcttaaaaa tctaagtaga gatttacatg tatctacttc tagtctgtgt atactactta
      301 ataaattggt tgagcaagag tatgtatata gggaagaaga tagtagagat agacgaaata
      361 ctttttatgg aataactaaa aatggagaaa agatattgga taatgaaata ctaaagtttg
      421 tatctataat aagtgataaa atggattgct tagatataga taataaagat aaattattta
      481 cttctttgga agagtctaaa aatataatag aacaattatt ttaaaaagga gaatttatta
      541 tgaatataaa gtattctaaa gagatattaa aaagtttaga aaatgctata acagtagtag
      601 ctctatttga gggagaagat gtttctacga catttgataa aataaatatg ataagcagaa
      661 attttaattc taaagaaaat agtatagata ctgtaacatt ggttgaaaat aacaacttgg
      721 taaatgtttg tcttgttggt tttggtaaga gagagttatt gaatagagaa aaaattagaa
      781 tcataggtgg taatttgtgt aaaaaattaa aaaatatatt gaaaaatgaa gcatataaga
      841 gtagtgatgt taatgtactt aatcttaatt taaatagtga tgaaataggt tcttttgtag
      901 aagggatatt acttggaaat tataagtttg ataagtataa gacaaaaagt aaggattttg
      961 aaagaaaaga agttgatact ataactatat ttactgaaaa agaagttgag tgtcaaatag
     1021 aaaaggctaa aatattagct aattcaacta taatagctag aaatctagtt aatgaaccat
     1081 caaacgtaat atatccaaaa acattagcaa tagaaacagt taaacttggt gcagagttcg
     1141 ggtttaatgt tgatgtttat gaagaagaaa aaataaaggc attgggaatg gatgcttttt
     1201 tcgccgtttc aagaggttca atcaataaac caagattcat agtaatgaga tactttggag
     1261 ataaagaaag tacagatata ttaggtctag taggtaaagg attgacttat gatactggtg
     1321 gatattcttt aaaatcaaat gcttctatgc tagatatgaa gacagatatg gctggtgctg
     1381 cgagtgttat aggtgctatg tgtgctatat ctcaaagtaa attaaagaaa aatgttatag
     1441 cagtagtggc tgcatgtgag aatgctttat caggaggttc atacaaacct ggagatataa
     1501 ttagttctat ggcaaagaaa actattgaag ttttaaatac ggatgccgaa ggtagactta
     1561 cattagctga tgcaatatac tacattataa acaatgaaaa agtaacaaag gtagttgatg
     1621 tagctacatt aactggtgct gctttaacat tgttaggaaa tgttgcaact cctatagtaa
     1681 caaataatga tgatttttat tgtgaattag aaaaagctgc cactttatca ggagaaaggg
     1741 tatggaagat gccaatttat gatgaattta aagatatgat aaaaggagaa gaagcagacc
     1801 ttaaaaacac tggtggtaaa aatgctggat gtataacagc aggagctttt attggtgaat
     1861 ttgttgggaa tactccttgg atacatatgg atatagcggg gacaagtaca tcatcaaaat
     1921 caattggata caaagcaaag ggggctacag gagaacctgt tagaacatta tactatttgg
     1981 cagaaatcta aaaaacaatt ataaaatata atttataaag ccacatggat atttttgtaa
     2041 tttaagatta tctatgtggc tttatttttt atttaagtaa attatttact ctttttcttt
     2101 ttctcaagta taaaagtatc tctattaaat aaaagagcag gtatgtacat aatactagta
     2161 acaaaaataa acgtaaaaat agcagttgtg ctatgactta aaaaaccata atagtattgt
     2221 gagttggtaa atgtgtttag taaaaatatg aatgaaaaat aaataaaaca tatagctaag
     2281 ttatctctaa gtgcatatct gttaactcta aattttctaa catcaaccat atatatatct
     2341 gcataaatta tcaagcactt agagaagata aatataaaat atattaagct aggaaattca
     2401 tagcccaagt ttttagaaaa aatgagttct ccaactgaac aaactaaact ccaagaaaaa
     2461 aatatattaa aaagatgata ctgcttaaaa attagtatgg ctatacacag atatatacag
     2521 atataattga ttccaattgg taaagagttt agagtactat aattacctga agaaataagt
     2581 aataattgct ctagagccat aattattaca agtaatgtac aaattatctt ctcaactgta
     2641 tagctataag gtaaaatatt tttggttagt tttggacaat agtataaaaa taatccaaat
     2701 ataaagagac ttacaaattg ttcactagaa aaaagaaaaa ttttaaacat ataacattct
     2761 ctcctataat aaatt
//
LOCUS       DAMJWP010000313         2758 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.320, whole genome shotgun sequence.
ACCESSION   DAMJWP010000313 DAMJWP010000000
VERSION     DAMJWP010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.320"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            367..1410
                     /locus_tag="PC700_003486"
     CDS             367..1410
                     /locus_tag="PC700_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582750.1"
                     /translation="MLKKWFGIVKKRQKSESVKEEGEVILKNEKILSEEKLIDEEGVV
                     VNIDNEILTKVEVVNDDNEIEEKIIEEDWLISENTIKLDDKEAIINDKNIELCKKEVQ
                     VESEKIGLNKFEGLDQNENYNLEKNVIEEKKVSECLTEEDLEYIKEIKIKRGKSIKAI
                     NLYTKEEWVFDTHIQCSKKLKVPLGYIRENLKYGYMDYFGDAINYLSEVLNIDEYCKG
                     EWSYLDNSKSPSEIFNILNNKIFSIRLSNEKRNEILTNDKIEALKMNYRFECIDEEYD
                     EYFKKYKSIIKRGGKKKVELVNKKGDILEIFKSLEECAIYLQKEKNEVIQMLKYGDTK
                     VGRNFIRYSLRSI"
     gene            1521..2663
                     /locus_tag="PC700_003487"
     CDS             1521..2663
                     /locus_tag="PC700_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2582751.1"
                     /translation="MSEEKNPKENTLDEPKNSDNVNSKKDDVSDEKNTDNKVKKREIK
                     LNNKTLKAIVAIVAVGAICLGGGYVFGKEAGRKLPATSKHYNSSEVVATIGDKKITGE
                     MLRKRMEPLFYLNAKTTMTDDEIRSYESNMIETMITSDILSSEAAKEKISITNDQLDA
                     QYNNLMSSIESAMGMTKEEFLKQFDLTEKYIKDDMKKELVASKYLNEKSKVTDKEAEN
                     YYNKNKSNYLQVRASHILIKTVDDKGKQVSSSKKAELKKEAEEILKKAQAGEDFATLA
                     KKYSEDSSAESGGDLGFFGKGQMVESFEKAAFALKKGEVSNKLVESDYGYHIIKKTDE
                     KYQPFEEVKSDLVSSLTSEKQNLLIQNLKEKYNVKITDKYQMKAKE"
BASE COUNT         1247 a          224 c          483 g          804 t
ORIGIN      
        1 atgtaaataa ttaatatata ataccattat gggcaataaa tacaaatcaa atgataatat
       61 agtctattta tgtaaatgtt atattgtatc ctaagtataa tagtaagttt tttgatagtg
      121 gtgtagaaag taaaaatcta ctagcctaaa ttgatgtatg gattagttta ggaacagtaa
      181 ttgcatatct taagtttgat gtcatgttgg gctactagaa ataggtcatc tttttcactt
      241 gaatatcttt tctttaaaca tgaggagtat catatcaaaa acatatgaaa attaagtgat
      301 ttataagaac agtaatattt tatatgatag tatagaatag taataaagta ttaggaggaa
      361 cattgagtgt taaaaaagtg gtttggtatt gtgaaaaaaa gacaaaaaag tgagtcagta
      421 aaagaagaag gtgaagtaat attaaaaaac gaaaaaatat tatctgaaga aaagttgata
      481 gatgaagaag gagttgtagt taatattgat aatgaaatat taactaaagt agaagtagta
      541 aatgatgata atgaaataga agaaaaaata atagaagaag attggttaat tagtgaaaat
      601 accataaaat tagatgataa agaagcaatt attaatgata aaaacataga attatgtaaa
      661 aaagaagttc aagttgaaag tgaaaaaata ggtttaaata agtttgaagg acttgaccaa
      721 aatgaaaatt ataatctaga aaaaaatgtt attgaagaaa aaaaagtaag tgaatgtttg
      781 acagaagaag atttagagta cataaaagaa attaaaataa aaagaggaaa aagtataaaa
      841 gctataaact tgtatactaa agaagagtgg gtttttgaca ctcatataca gtgtagtaaa
      901 aaactcaaag ttccattagg atacataaga gaaaatttaa aatatggata tatggattac
      961 tttggagatg caataaatta tttaagtgaa gtattaaata tagatgaata ctgcaaaggt
     1021 gaatggagct atctcgataa tagcaaatct ccatctgaga tatttaatat tctaaacaat
     1081 aaaatattta gtataaggct ttctaatgaa aaaagaaatg aaattttgac aaatgataaa
     1141 attgaagcat taaaaatgaa ttatagattt gaatgtattg atgaagaata tgatgaatat
     1201 tttaaaaaat ataagtctat aatcaaaagg ggtggaaaga aaaaagttga attagtaaat
     1261 aaaaaaggtg acattttaga aatatttaag tctttagaag aatgtgccat ttatttgcaa
     1321 aaggaaaaga atgaagttat acaaatgtta aaatatggag atacaaaagt aggaagaaac
     1381 tttataaggt atagtttgag aagtatttaa tttcatttta aattcataag agtttcattt
     1441 ctgattcaca catttaatat tattttaatg taaaataata aaggtgaagt attgcacaat
     1501 taaaattaag gagggtatta atgtctgaag aaaaaaatcc aaaagaaaat acattagatg
     1561 aacctaaaaa ttcagataat gtaaattcta aaaaagatga tgtgtcagat gaaaaaaata
     1621 ctgataacaa agttaaaaaa agggaaataa agctaaacaa caaaacactt aaagctattg
     1681 tagcaattgt tgcagtagga gctatatgtt taggtggagg ctatgttttt ggtaaagaag
     1741 ctggaagaaa attgcctgct acttcaaaac actataatag cagtgaggta gtagctacta
     1801 ttggggataa aaaaataaca ggtgaaatgt taagaaagag aatggagcca ttattttatt
     1861 taaatgcaaa aactactatg actgatgacg aaatacgttc atatgaatca aatatgattg
     1921 aaactatgat tacatctgat atattatcct ctgaagcagc aaaagagaaa ataagtataa
     1981 ctaatgacca actagatgct cagtataata atttaatgag tagtatagaa tcagcaatgg
     2041 gaatgactaa agaagagttt ttaaaacagt ttgatttgac tgaaaaatat ataaaagatg
     2101 atatgaaaaa agagctagta gcatcaaaat atttaaatga aaaatcaaaa gttactgata
     2161 aagaagcaga gaactattac aataaaaaca agagcaatta tttacaagtt agagcttcac
     2221 atattttaat aaaaactgtt gatgataaag gtaagcaagt tagtagtagc aaaaaagctg
     2281 aacttaagaa agaagctgaa gaaatactta aaaaagccca agctggggaa gattttgcta
     2341 ctctagctaa aaaatattct gaagatagta gtgcagaatc aggtggagat ttaggtttct
     2401 ttggtaaagg tcagatggtg gaatcttttg aaaaagcagc ctttgcattg aagaagggag
     2461 aggtatctaa caaacttgta gaaagtgatt atggatatca tataattaag aaaactgatg
     2521 aaaaatatca accatttgaa gaggtaaaat cagatttagt aagcagttta acatcagaaa
     2581 aacaaaacct acttatacaa aatttaaaag aaaaatataa tgttaaaatt acagataaat
     2641 atcaaatgaa agctaaagaa tagataaagt caatttatta aaaaaaagaa aaattaactg
     2701 tatggataaa agctattttt actctcaata tagagagtct aaagtagctt ttattatt
//
LOCUS       DAMJWP010000314         2749 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.321, whole genome shotgun sequence.
ACCESSION   DAMJWP010000314 DAMJWP010000000
VERSION     DAMJWP010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.321"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(180..563)
                     /locus_tag="PC700_003488"
                     /pseudo
     CDS             complement(180..563)
                     /locus_tag="PC700_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     regulatory      790..887
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            942..1118
                     /locus_tag="PC700_003489"
     CDS             942..1118
                     /locus_tag="PC700_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582752.1"
                     /translation="MFGNKMDKCTHVLTAYISGSYDYCNFLDTQLDDFILEYGESVVE
                     SCLHQVMVLVSKYN"
     gene            1344..1544
                     /locus_tag="PC700_003490"
     CDS             1344..1544
                     /locus_tag="PC700_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDF2582753.1"
                     /translation="MNNGIVKWFNNEKGFGFISMEGRDDVFAHFSAIQTSGFKSLEEG
                     QQVSFDIVKGARGPQAENITIL"
     gene            complement(1708..2007)
                     /locus_tag="PC700_003491"
     CDS             complement(1708..2007)
                     /locus_tag="PC700_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582754.1"
                     /translation="MGFYINYVVCKVKILFSSFSKIILFYINYVVCKLFITLKAILLK
                     TQIIVTTHSPHILQIDSKEEMIVLDMAESGNVYKKELELGEYGVLGWTNEGLYLH"
     gene            2229..2411
                     /locus_tag="PC700_003492"
     CDS             2229..2411
                     /locus_tag="PC700_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDF2582755.1"
                     /translation="MFKFYNNATLMFLSGTNIKTISERLGHADIKITMNSYSQFLEEM
                     DREAFENLSKILFIVF"
     gene            2482..>2749
                     /locus_tag="PC700_003493"
     CDS             2482..>2749
                     /locus_tag="PC700_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582756.1"
                     /translation="MNIIKSITLLLTLLIVILMTGCSPRESASEVAKKYIDKMNSGDY
                     ETAYSLLSKDSKKKISIENLEEFQNILVSTKRIISYKIGKEKIIK"
BASE COUNT          996 a          337 c          355 g         1061 t
ORIGIN      
        1 tatatatggc tatttttcaa cattttacaa tatatataac atttattttt ttaacttttg
       61 tcagtaaaat gtcagcaaga cactttatcc acaattttta tataagtttt ccacaactta
      121 tccccagtat tatccacata aaaaaggtag ctcttatatt attagctacc ttttatttat
      181 taatcttcat atttccagct ttttatccaa cttcctattt ctattatttt ttcttcagtt
      241 atttcttctc catcatgtaa ttttgaaatt aattctgata aaggattgtt atgtaaacct
      301 ctaaaaactc tttttccctc tattttgaga tattctctct cttttattaa tactgtatag
      361 tgtacatgcc tcccaattct ctatgatatt aaaaaacctt ttttggttaa tctagctaaa
      421 agtataattg tagttgtatg cttccaacca tatgtttttt ctatttcatc tgcaacatct
      481 tttgatatta cagttttata accagtattc catatgcatt tcataatttt taattctgta
      541 tctggtaatt ttttatttag cataatatat tccttctttc aatttatgtc acttcttcaa
      601 atttatctag cttaaattat aataggagtt ttttagaaca aaaactatag ttaattttta
      661 ccaggtactc tacgtgttta gtctgttttt taagtgtaaa ataatgttca tatctataaa
      721 tttatttaac tttcaaacaa tcctgttgaa taataactaa ttctattgta ttattttagt
      781 tgtagaataa aactaaatcg gcaaaactag agaaatttag tgacgcaaag ctatagggac
      841 taagacttat ataaatatct tatgagttat gtcagccagt tgccaaagag atagtgttct
      901 ttttgttttt atgaaagttt tttaggggga taataattta tatgtttgga aataaaatgg
      961 ataaatgtac acatgtattg actgcttata ttagcggttc gtatgattat tgtaattttt
     1021 tagatacaca attagatgat tttatattag agtacggaga aagtgtagta gagtcttgtt
     1081 tgcaccaagt gatggtattg gtaagtaagt ataattagat agacattgat ttctagagaa
     1141 ccctttttag ttttatctac atttatatta atatatagca atgtaaaacg cttaaatata
     1201 tttttaatat taagaataac taaaatgttg actaaaaaat ataattaagt agacatttct
     1261 attaatctat gatacaatag tatttgtggt aatacacaat taaatcaagt atatcttgat
     1321 ataggttaat aggagtattt ttaatgaata atggaatagt gaaatggttt aataatgaaa
     1381 aaggattcgg ttttatatca atggaaggta gagatgatgt gtttgctcat ttctcagcta
     1441 tacaaacttc aggatttaaa tcattagaag aaggtcaaca agtaagcttt gatatagtta
     1501 aaggtgctag aggtcctcaa gcagaaaata taactatatt ataatatata gatttttcgg
     1561 gaaagactct ttttagagtc tttttttatt gttagtatat gtaagataat cattggtgaa
     1621 gtaaataagg tatattgagt aatgttcttg atagtttata tgctaatgaa tttcaaaaaa
     1681 ataagcactc ctataaaaag agtgctctta atgtaaatat aagccttcat tggtccagcc
     1741 taataccccg tattctccaa gttctaactc ttttttatat acattaccac tttcagccat
     1801 atctaacaca atcatttctt ctttagaatc aatttgtaaa atatgaggac tgtgtgttgt
     1861 gacaattatt tgtgttttta ggagaattgc ttttaatgta ataaaaagtt tacatacaac
     1921 atagttaata taaaacaaaa ttatttttga gaatgagcta aataaaatct taactttaca
     1981 taccacatag ttgatataaa accccaaaat aaacttagca tctccaatgt ttacacatat
     2041 acaatgctct taaaattgca gtgaacatgc aatagtgcaa ttgataatac ttatcatata
     2101 ttctcaatac cttatatttc aactgctaaa cctcaatttg tcacaaatat cgctcactgc
     2161 aaaatttcta tatttttatt atatcatagc atataattaa tattttcaat taatttttac
     2221 atttaattat gtttaaattc tataataatg caactttaat gtttctttct ggaactaata
     2281 taaaaactat ttcagaaaga ttaggtcatg cagatataaa aataactatg aatagctact
     2341 ctcagttttt agaagagatg gacagagaag catttgaaaa tttgagcaaa atactattta
     2401 ttgtatttta aagaagatga cttattcaat atttgatgct agaatataat taataaaaat
     2461 ttaattatag gtgattgtat tatgaacatt attaaaagca taactttact cttaacatta
     2521 ttaattgtga tattaatgac aggatgttcc ccaagagaaa gtgctagtga agttgcaaaa
     2581 aaatatattg ataagatgaa ctctggtgac tatgaaacag catatagttt attaagtaaa
     2641 gatagtaaaa aaaaaattag tatagaaaat cttgaagaat ttcaaaatat tttagttagt
     2701 acaaaaagaa taattagtta taaaataggt aaagaaaaaa tcataaaaa
//
LOCUS       DAMJWP010000315         2746 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.322, whole genome shotgun sequence.
ACCESSION   DAMJWP010000315 DAMJWP010000000
VERSION     DAMJWP010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.322"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(<1..531)
                     /gene="mobB"
                     /locus_tag="PC700_003494"
     CDS             complement(<1..531)
                     /gene="mobB"
                     /locus_tag="PC700_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HDF2582757.1"
                     /translation="MKGHNFMYNILSVVSYSGVGKTTLIEGIIKELNKKGYRVGVIKH
                     TCHDFDMDEEGKDTYKHRKSGARKVCIISDKRVAYIEELKEKNPLYKMIQLYEDMDLI
                     IIEGYKNYRFKRLEVTRKGKYEDILSNKSDLIGIVSDIEYKLNIEQFNLNDYKNISKY
                     IESCIKNEILSISNVEI"
     gene            complement(536..1717)
                     /locus_tag="PC700_003495"
     CDS             complement(536..1717)
                     /locus_tag="PC700_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HDF2582758.1"
                     /translation="MIELEDAIKIIEKNVLPINRVKIVNLIDAVNKVVAEDIYSTIDS
                     PPFDRSPYDGYAYNANSENRNLKVIGTLYAGEVFNKVLNKNEAVKIMTGGKIPQGANC
                     VIKKEDVTVIGDMIILNVKLKEYDNYIFKGEDIKKGQLIINKNQNIGYDGIGVLASLG
                     ISKVTVYDDLKVGILNTGTELQDINETLENGKIYDSNRYTLYSRLLKLGIQADTICNC
                     SDDILELEKKVKNMLQSVDFLITTGGVSVGDKDLIPDVFKKIGATELFWKINIKPGGA
                     CYVGILGEKLLFGLSGNPHAAIVVFDNVIVPVIEYLTYKKGIHHIKAIFKGEFNKLSN
                     KDRFVSGKLYTSEGKLYVELNDKKDAKARLSATLRSNCMIKIKKNETIKENQLVEIVI
                     R"
     gene            complement(1928..2662)
                     /locus_tag="PC700_003496"
     CDS             complement(1928..2662)
                     /locus_tag="PC700_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582759.1"
                     /translation="MKLKRSLVCVTILGIILVGCHKENTKEKNQVASKATQQKTMTKV
                     QNDVNEIMNKDYKYIIKNMGIPYNTFYYIKPKVLKESNTMQDINTSSYMTLVYPKYTG
                     NDELDGSALYVDINENKVVNVETNSFSSQGISVIDAESSVVIEKSDHEKSAVSLENFR
                     RIDLGEYVGVEESRINEIVGDANYDLTAYNHEGSKVVKSYRLKEDNKILKKEVLTISI
                     VDNKIKSIKTIESDKIVKIIKGTLLE"
BASE COUNT          855 a          500 c          218 g         1173 t
ORIGIN      
        1 tatttcaaca ttgcttatac ttaaaatttc attttttata caagactcta tgtactttga
       61 tatattttta taatcattta aattaaactg ttcaatattc aatttatatt ctatatcact
      121 gacaatacct atcaaatcag acttattaga taaaatatcc tcatacttac cttttctagt
      181 tacttccaat cttttaaatc tataattttt atatccttct atgataatca agtccatatc
      241 ttcatataat tgaatcattt tatataatgg attcttctct tttagttctt caatatatgc
      301 tactcgttta tctgatatta tacagacttt tctcgcccca gattttctat gtttataagt
      361 atcttttcct tcctcatcca tatcaaaatc atggcaggta tgtttaataa ctccaacacg
      421 ataccctttt ttatttaatt cttttattat gccttcaatc aacgtagttt tacctactcc
      481 tgaatatgag acaactgaca atatattata cataaaattg tgtcctttca taaaactatc
      541 taataacaat ctccactaat tgattttctt ttattgtctc attcttttta atcttaatca
      601 tacaattact tcttagagtt gcagataatc tagcttttgc atctttctta tcatttagct
      661 ctacatacaa tttaccttca cttgtatata gttttccact tacaaatcta tctttatttg
      721 aaagtttatt aaattctcct ttaaatatag cttttatatg atgtattccc ttcttatatg
      781 ttaaatactc aataacagga actatcacat tatcaaatac cactattgct gcatgtggat
      841 taccagaaag accaaataat agcttttctc ccaatatacc tacataacaa gctccaccag
      901 gtttaatgtt tatcttccaa aataattctg ttgcccctat ttttttgaat acatctggaa
      961 ttaaatcttt atctccaaca gaaacaccac ctgttgtaat taaaaaatct acactctgta
     1021 acatattctt tacctttttt tctaactcca atatatcatc agaacaattg catattgtat
     1081 ctgcctgtat tccaagtttt aataatcttg aatataaggt atatctatta ctatcgtata
     1141 ttttaccatt ctctaaagtc tcatttatat cttgtagttc tgtacctgta ttcaaaattc
     1201 caacttttaa atcatcataa acagttactt tggatattcc aagacttgct aatactccaa
     1261 taccatcata tcctatattc tgatttttat taataatcaa ttgccccttt tttatatcct
     1321 cacccttaaa tatgtaatta tcgtattcct ttaattttac atttaaaatt atcatatcac
     1381 ctataaccgt tacatcttct ttttttatta cacagtttgc accctgtggt atctttccac
     1441 cagtcattat cttcactgct tcatttttat ttaatacctt attaaatact tctcctgcat
     1501 acaaagtccc tataaccttt aaatttctat tttcagaatt tgcattatat gcataaccat
     1561 catagggtga cctatcaaat ggaggactat caattgttga atatatatct tctgcaacta
     1621 ctttatttac tgcatctata agatttacta tcttgaccct gtttattggt aaaacatttt
     1681 tttcaataat ttttatggca tcttctaatt ctatcatttt aaatactccc caatatattt
     1741 tttatttaag atatttctat gtaatatatt atagattata aaagttggaa atacttttat
     1801 cttatattta attataacta atagttatct ataagtgcag ttcaaatttt ataaatctaa
     1861 taaaaaaact gttcccagct agaaatttcc ttctatcttg aaacagcatc atatgcttta
     1921 gaatatttta ttctaataaa gttcctttta ttatttttac aattttatca ctttcaatag
     1981 tttttataga ttttattttg ttatccacaa tacttattgt aagtacctct ttttttaata
     2041 ttttgttatc ttctttcaat ctataactct taactacttt actaccttca tgattatatg
     2101 ctgttaaatc ataattcgca tctcctacaa tttcatttat tctagactct tcaacaccaa
     2161 catactctcc caggtcaata cgtctaaaat tttctagtga tacagccgat ttttcatggt
     2221 cacttttttc aatcacaaca ctagattcag catcaatgac tgatatacct tgtgatgaga
     2281 aagaattggt ttcaacattg acaactttat tttcgttaat atcaacatat aaagcacttc
     2341 catctagttc atcatttcca gtatattttg gataaactaa tgtcatataa cttgatgtat
     2401 ttatatcttg cattgtattc gattctttta aaactttagg ttttatataa taaaatgtat
     2461 tataaggtat acccatattc tttataatgt acttataatc tttattcata atttcattaa
     2521 catcattttg aacttttgtc atagtttttt gctgtgttgc tttacttgct acttgatttt
     2581 tctctttagt attttcttta tgacatccaa caagtataat acctaaaata gttacacaaa
     2641 ctaatgacct ttttaacttc ataaatcaat cctcctttct taaattttat agtatttatc
     2701 tatatctcaa tttacagtta tctctttttt caataattat atttat
//
LOCUS       DAMJWP010000316         2690 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.323, whole genome shotgun sequence.
ACCESSION   DAMJWP010000316 DAMJWP010000000
VERSION     DAMJWP010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.323"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            119..2254
                     /gene="rnr"
                     /locus_tag="PC700_003497"
     CDS             119..2254
                     /gene="rnr"
                     /locus_tag="PC700_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDF2582760.1"
                     /translation="MIPGLKESLLGLINESAYNPLKKEELAEIFDIHSSEMPMFYNFL
                     NELEEDGYICFTKKGKIVSPNQMGYFVGKFVSHKKGFGFVESDVEYTQDLFISSDNIN
                     GAMHNDRVMAEIVVPATEEKRAEGRIVKVIKREITRIVGTFQSSKTFGFVTPDNKKFT
                     KDIYIPKKHFSGAVDNDKVVCEITVWPQEDRKPEGKIIEILGQKGERGVEIDSIIREH
                     GLPEEFPKKVLQEAEAVAVEIPQEEIKRRRDLRDLNIFTIDGDDAKDLDDAISIEVLP
                     NGNFKLGVHIADVTHYVREKNKLDKEALKRATSVYLVDKVIPMLPKTLSNGVCSLNPF
                     EDKLTLSIFMEIDHKGNVVKHEICESVINSKARMTYTEVSDILEKDDEKLKKTFEHVV
                     EDFKNAEILAKILMSRREKRGAIDFNFPEAKIILNGKGEVVDIKPYERRISNKIIEEF
                     MLISNETIAEHFYWMGIPFVYRIHETPSLEKMEELSKFISTFGYIIKGDKEEVHPKAL
                     QGIIEKIKGKKEEGAISTIMLRSLKQAKYSPECSGHFGLAAKFYCHFTSPIRRYPDLQ
                     IHRIIKESLNNKISGKRQEQLTTIVDYASVQSSEKERKAELAERDVHDFYKALYMKDK
                     VGQEFEGVVSSVTSFGIFVELDNTIEGLIRLANMNDDYYIYDESSYSVLGERTKKSYK
                     IGDLVKIKVESVNVDFKEIDFEIVEKIEE"
BASE COUNT         1181 a          253 c          499 g          757 t
ORIGIN      
        1 ttgtaataat aataagaaaa tatagaaaat tatgtgataa gtaaaaaatg ttttatctga
       61 agaaatataa aagacaatat aaataataat ataatgaaaa gtaaaacagg agggaaaaat
      121 gataccagga cttaaagaaa gtttgctagg attaataaat gaaagtgcat ataatcctct
      181 aaaaaaagaa gaattagcag aaatatttga tatacattca tcagaaatgc caatgtttta
      241 taatttttta aatgaattag aagaagatgg ttatatctgc tttactaaaa aaggaaaaat
      301 agtatctccc aatcaaatgg gatactttgt tggaaaattt gtatcacaca aaaagggttt
      361 tggatttgta gaatctgatg ttgaatatac acaggattta tttatctcaa gtgacaatat
      421 aaatggtgct atgcataacg atagagttat ggcagaaata gtagtacctg caactgaaga
      481 aaagagagca gaaggaagaa tagtaaaagt aataaagaga gaaataacta gaatagtagg
      541 tactttccaa tcaagtaaaa cttttggatt tgtaacacca gacaacaaga aatttactaa
      601 agatatatat atacctaaaa aacattttag tggagcagta gataatgata aggtagtatg
      661 tgaaattaca gtatggccac aagaggatag aaaaccagaa ggcaaaatta tagagatact
      721 aggtcaaaaa ggtgaaagag gcgtagaaat agattctata ataagagaac atggtcttcc
      781 agaagaattt ccaaagaaag tattgcaaga agcagaagct gtagctgttg agattcctca
      841 agaagagata aaaagaagaa gagatttaag agatttaaac atatttacta tagatggaga
      901 tgacgcaaaa gacttagatg atgccatatc aatagaagta ttacctaatg gaaacttcaa
      961 actaggagtt cacatagctg atgttacaca ttatgtaaga gagaaaaata aacttgataa
     1021 ggaagcctta aaaagagcta catcagttta tttagtagat aaagtaattc caatgttgcc
     1081 aaaaactctt tcaaatggag tatgtagctt aaatccattt gaagataagt taactctttc
     1141 tatatttatg gaaatagacc ataaaggaaa tgtggttaaa catgaaatat gtgaatctgt
     1201 aataaattca aaagctagaa tgacttatac agaagtatct gatatattag aaaaggatga
     1261 tgaaaaactt aaaaagactt ttgaacatgt tgttgaagat tttaaaaatg ctgaaatttt
     1321 agctaagatt cttatgtcaa gaagagaaaa aagaggagct atagacttca atttcccaga
     1381 ggcaaagata attctaaatg gaaaaggcga agttgtagat ataaagccat atgaaagaag
     1441 aatatctaat aagataatag aagaatttat gcttataagt aatgagacaa ttgccgaaca
     1501 tttttactgg atgggaatac cgtttgtgta tagaatacat gagacaccat ctttggaaaa
     1561 aatggaagaa ttgagtaagt ttatatctac atttggatat ataataaaag gtgataaaga
     1621 ggaagtacat ccaaaagcat tacaaggtat aatagagaaa ataaagggca aaaaagaaga
     1681 aggagctatc agtactataa tgcttcgttc tttaaaacaa gctaagtact caccagaatg
     1741 tagtggacat tttggattag cagctaaatt ctactgtcac tttacttcac caataagaag
     1801 atatcctgac cttcaaattc atagaataat aaaagaatct ttaaataata aaataagtgg
     1861 aaaaagacaa gaacagctta ctactatagt ggattatgca tctgttcaat catccgagaa
     1921 agaaagaaag gcagagttag ctgaaagaga tgtacatgat ttctataagg ctctttatat
     1981 gaaggataaa gttggtcaag aatttgaggg tgttgtatct agcgtaactt catttggaat
     2041 atttgttgag ttagataata ctatagaagg attgataaga cttgcaaata tgaatgatga
     2101 ttactatatt tatgatgaga gttcttatag tgttttagga gaaagaacta agaagtcata
     2161 taagatagga gaccttgtta agataaaggt tgaaagtgtt aatgtagatt ttaaagaaat
     2221 tgactttgag atagttgaaa aaatagagga gtagataaaa cagctaaaat aagagataac
     2281 aagttgtagt atttatgata tgtataaata tatagtgcac aatactctat aaaataaatg
     2341 ataaaaatag gtaattaaga aatatctaat ggaaatagtt aacttaaaga taaataatta
     2401 agaaatattt aatgaaagta gctaattgaa aaataagtaa ttgagaaata tttaatagaa
     2461 agagctaact gaaagataag taattaagaa acatttaata gaaaaagcca actgaaaaat
     2521 aaataattaa gaaatgttta atggaagtga ctaattgaaa aataagtaat taagaaatgc
     2581 ttaatagaag tggctaattg aaaaataagt aattaagaaa tgtttaatag aagtggctaa
     2641 ttgaaaaata agtaattaag aaatgtttaa tagaagtggc taattgaaaa
//
LOCUS       DAMJWP010000317         2678 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.324, whole genome shotgun sequence.
ACCESSION   DAMJWP010000317 DAMJWP010000000
VERSION     DAMJWP010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.324"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            302..2113
                     /locus_tag="PC700_003498"
     CDS             302..2113
                     /locus_tag="PC700_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HDF2582761.1"
                     /translation="MKKKYDFLIYSAIWLVIIIFFIFSLYHSIEHIKIISHTGTIRSE
                     TQKVVKQELNNERNDDLIKRLDNILIKLRTGNGENGFQRCDNKEFQQKLNQMDSMWES
                     MKKEIIKVRNGASGDKLYKLSEEYSVLSNQIVFISEKHSNAKLYSFATALFIYLIFST
                     ISLLIWEYYNKKRFKRIFYTDNLTKIKNQVAFENRAIEILYNASNKEYVLLNIDIDNF
                     KYINDTHGYEYGDKVLIIVAAALSKTFNIKETCARIGSDNFVILAKYRDSLLEDIREM
                     LTNAIISELDMNVTQTISYCIGAYLVEIDNLGYKSINSIMDKANIAHKVSKTRGISST
                     VWYNENLLKQLQMENSIYNYMYKAIENEEFHMYLQPKFQISSLNVVSAEALVRWFSPE
                     LGFLSPDEFIPLFEKSGFIIELDFYMLKKACSFVKKTFMKKNQYTYPIAVNFSRVTIY
                     QNSFYQRFLDTVKEYEIPFKYIEIEVTESAFNEISQPVISILEELKKLGFLISMDDFG
                     SGYSSLSLLCSLSINGLKLDKSLLKETFNREKVYSIIQCIIEMSHRIGMSVVCEGIET
                     KKDLEFLNTVKCDVGQGFYFSKPIEEKEFFNKYVTKK"
     gene            complement(2201..>2678)
                     /locus_tag="PC700_003499"
     CDS             complement(2201..>2678)
                     /locus_tag="PC700_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582762.1"
                     /translation="KKDNSNFNYTNYYMEFEDIPEYYEVNFNNYLYIITRFSFIPDGT
                     EIFFTWEEIHYWDEKVATEKTFINGETLVDSYNFPNNEVKDYIQKRESLYIETKKYYE
                     EKGLKIERHALGSEDGEYVELTNDSILYLLDIEAVEIREQSKNIEEFLYKYKKRFK"
BASE COUNT         1004 a          279 c          393 g         1002 t
ORIGIN      
        1 acataaagac attatacaaa tatattattg gggtgatatt ttataagaaa agaagtgatg
       61 cagtaaatta attgagcaat ttaaaaatga tttaatgata gtgttgatga atgtttaaaa
      121 agaaatttta gaaaaacgta tattgctgtt tgtgttggag gtgatgaatt tgtataattt
      181 atagggtata aatgattaaa ctacttaaga taaatacata tcactgtaaa atattgataa
      241 tcatttttta taagagattg attatttgca aattatatat attacaaatg aggtaacaat
      301 aatgaagaaa aaatatgatt ttttaattta ttctgctatt tggctagtta ttattatttt
      361 ttttatattt tctctatatc atagtataga gcatattaaa attataagtc acactggtac
      421 tattcgcagt gaaactcaaa aggttgttaa gcaagaattg aataatgaaa gaaatgatga
      481 cttgataaaa cgactggata atattctgat aaagctaaga acaggtaatg gagaaaatgg
      541 atttcaaaga tgtgataata aagaatttca acaaaaacta aatcaaatgg attctatgtg
      601 ggaatctatg aagaaagaaa ttataaaagt tagaaatggt gcttcaggtg ataaattata
      661 taaattgagt gaagagtatt ctgtgttgtc taatcaaatt gtttttatat ctgaaaaaca
      721 ctctaatgca aaattgtatt cttttgctac tgcactattt atttatctta tattctcaac
      781 aataagtctt ttaatttggg aatattataa taaaaaaagg tttaaaagaa tattttatac
      841 tgataattta acaaaaataa aaaatcaggt tgcatttgaa aacagagcga ttgaaatttt
      901 gtacaatgca tctaacaaag agtatgtact gctaaatatt gatattgaca attttaaata
      961 tattaatgat acacatggat atgaatatgg agataaagtt ctaataatag ttgcagctgc
     1021 actttctaaa acttttaata tcaaagaaac ttgtgcaaga attggttcag ataattttgt
     1081 aatacttgca aaatatagag attctctttt agaggatatt cgggaaatgc taactaatgc
     1141 tataatctct gaattagata tgaatgtaac tcaaactata tcttattgta taggagcgta
     1201 cttagttgaa attgataatt taggttataa atccatcaat tctataatgg ataaggcaaa
     1261 tattgcacac aaggtaagta aaacaagagg aatttcctcc acagtatggt ataatgaaaa
     1321 tctgttaaaa cagttacaaa tggaaaatag tatttataat tatatgtata aagcgataga
     1381 aaatgaagaa tttcatatgt atttgcaacc taaatttcaa atatcttctt taaatgttgt
     1441 aagcgcagaa gctcttgtaa gatggttttc tccagaactt ggttttttat caccagatga
     1501 atttattcca ttgtttgaaa aaagtggatt tattattgaa ttggactttt atatgttaaa
     1561 aaaagcgtgt tcatttgtaa aaaaaacgtt tatgaaaaaa aatcaatata catatccgat
     1621 tgcagttaat ttttcaaggg ttactattta ccaaaatagc ttttatcaaa ggtttttaga
     1681 tactgttaaa gaatatgaaa ttccttttaa gtatatagag atagaggtaa ctgaaagtgc
     1741 ttttaatgaa atatctcaac cagttatttc gattttagag gagcttaaaa agttaggatt
     1801 tttaatttcg atggatgatt ttggatcagg atactcttca ttaagtcttt tatgttctct
     1861 atctattaat ggattgaagt tagataaaag cttacttaaa gaaacattta accgggagaa
     1921 agtatatagt attattcagt gtattattga aatgtcacat cgtataggca tgtctgttgt
     1981 atgtgaaggt attgaaacta aaaaagactt ggaattttta aatacagtta aatgtgatgt
     2041 aggacaaggg ttttatttct ctaaaccgat agaagaaaaa gaatttttta acaaatatgt
     2101 aactaaaaaa taatataatt atttttatca gtctatacat tgagtcattt tatatttcgt
     2161 taaatgactc aatgtattgt tatgtatttt aaaatataaa ttatttaaaa cgttttttgt
     2221 acttatatag aaattcttct atattttttg attgttctct tatttcaact gcttctatat
     2281 ctagtaaata aagtatagaa tcatttgtaa gttcaacata ttcaccatct tctgaaccga
     2341 gtgcatgtct ctcaattttt aatccttttt cttcataata tttttttgtt tctatatata
     2401 atgattctcg cttttgtatg tagtctttca cttcattatt tggaaagtta tatgaatcca
     2461 ctaatgtttc gccatttata aaagttttct cggtagcaac tttttcatcc caataatgga
     2521 tttcttccca agtaaaaaat atctcagttc catctggaat gaatgaaaac ctggtaataa
     2581 tatataaata attattgaaa tttacttcat aatattcagg aatatcttca aattccatat
     2641 agtaatttgt ataattaaaa ttactattat cttttttt
//
LOCUS       DAMJWP010000318         2658 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.325, whole genome shotgun sequence.
ACCESSION   DAMJWP010000318 DAMJWP010000000
VERSION     DAMJWP010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.325"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            121..519
                     /locus_tag="PC700_003500"
     CDS             121..519
                     /locus_tag="PC700_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2582763.1"
                     /translation="MDTLGKRIAYLRNSKKLTQRKLMDILKFENLGKYETGDRKPNCD
                     ILMSIADYFNVTTDWLLYGKEKVNVNSSVKEDKEDYLHVTNDEMMILNLYRQLNERDK
                     IKIEGILELKISEYKDLKKHSSNNNEDKMV"
     gene            complement(736..945)
                     /locus_tag="PC700_003501"
     CDS             complement(736..945)
                     /locus_tag="PC700_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582764.1"
                     /translation="MCKLDSDGVIECCRAIDDFITALSNIKSLNMERLNTLTKYSSTC
                     SILLKEGNYEGCTIVYRKMLEELKT"
     gene            complement(977..1186)
                     /locus_tag="PC700_003502"
     CDS             complement(977..1186)
                     /locus_tag="PC700_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2582765.1"
                     /translation="MDARKKWIPFLGVQVKQRLIELNMTQRELAKKIGVNENYLSAIL
                     NGRRTGKKYKSSIYQLLNIEYSEDD"
     gene            1485..1904
                     /locus_tag="PC700_003503"
     CDS             1485..1904
                     /locus_tag="PC700_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2582766.1"
                     /translation="MDTLGERIVYLRKAKNLKQYELEEMLGCDNLSKFERNIRKPNYE
                     ILKSIAEIFNVSVDWLLNGDNLSHKSDLICDSSSNYPLNSINSNEIKLLNNFRKLNDY
                     DKAKIEGMIELKLHEYEKEKDLGKIEYNKNKDEKIDK"
     gene            2132..2587
                     /locus_tag="PC700_003504"
     CDS             2132..2587
                     /locus_tag="PC700_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582767.1"
                     /translation="MGLRDKFAQSFARSKTMSGPEKKANEIMGKLLLKKAILPIVLMF
                     VIIIAGAMLKINSWVTLGINLVIAVGAFFYIRNSSKKYQNFKPYVGNLISLEKKGKKE
                     YVAIIKQGKLPVKLQIAYGGEDLEHVKKNQMVQISYNPDAKIAILVNRQ"
BASE COUNT         1003 a          321 c          345 g          989 t
ORIGIN      
        1 tattaattta agcaaaatgt catttagact ttaatttagt agttttatat tataaattta
       61 tatattttta atttcaaatg tcatttagac attactcata aaaagaaagg agtgtatttt
      121 gtggatactt taggaaaaag aattgcttat ttgagaaact ccaaaaaact tactcagcgc
      181 aaacttatgg atattttaaa atttgaaaac ttaggcaagt atgaaacagg agatagaaaa
      241 cctaattgtg atatactaat gtcaatagct gattatttta atgttacgac tgactggctt
      301 ctctatggta aagagaaggt aaatgtcaat agtagtgtaa aagaagataa agaagattac
      361 ttacatgtta ctaatgatga aatgatgata ttaaatctat acagacaact aaacgaacgt
      421 gataaaataa aaatagaagg aatacttgaa cttaagatat ctgaatataa agacttaaaa
      481 aagcattcct ctaataataa tgaagataaa atggtttaaa catacataat ttgatacgat
      541 tttacatcat ctattaacct tctttttttg tagtatatta ctaaaatatg gatattggat
      601 tatttaaaaa caagtatagt ctaatttcaa gcacatttat aatatttaga ttatcttatc
      661 catattttag tttaagtact ttgaaaaata taatacattt ctatgtataa tttatattcc
      721 taagaaatgc attactcatg tttttaattc ttccaacatc tttctataca caattgtaca
      781 tccttcataa ttcccctctt taagaaggat tgaacatgta ctagaatatt tagttaaagt
      841 atttaatctt tccatattta agctttttat attactaagt gctgtaataa aatcatcaat
      901 tgctctacaa cactctataa ccccatctga atccaatttg cacacaagaa tattcaccta
      961 ctttatatac tatttattaa tcatcttctg aatattctat attaagtaat tgataaattg
     1021 atgatttata ttttttacct gttcttcttc catttaaaat agctgacaaa tagttttcat
     1081 taacacctat tttcttcgct aattcccttt gagtcatatt taattcaata agtctttgct
     1141 tgacttgcac tcccaaaaaa ggtatccatt tttttcgagc atccatttat caacttcctt
     1201 tctaagtgat ttatatttac ttttatatta ctttagctta cgcaccccct ctatttagta
     1261 tatattttta atatgtacac atattaagta tataaattta tttaatacta aataagattt
     1321 tttgattact ttgtgctaaa attaggatgt aaattcgtat cattaagtta attataaagt
     1381 cattttgacc ttaagtcaat ggtttttatg tcattttgac ctttaatttg aaactattta
     1441 catcttaaaa agtctttttg acattaattg aatggagagt gtttatggat actttaggag
     1501 aaagaattgt ttatttgaga aaagcaaaaa accttaaaca atatgaacta gaagaaatgt
     1561 taggctgtga caacttaagt aaatttgaga gaaacattag aaaaccaaat tatgaaatat
     1621 taaaatctat agctgagata tttaatgttt cagtagactg gctattaaat ggagataatc
     1681 tatcacataa atcagattta atttgcgatt cctcctcaaa ctatccactt aatagcataa
     1741 actctaatga aataaagctt ttaaacaact ttagaaagtt aaatgattat gataaagcca
     1801 aaatagaagg tatgattgaa ttaaaacttc atgaatatga aaaagagaag gatttaggaa
     1861 aaattgagta taacaaaaat aaagatgaga aaatagataa ataattttta tgttgctttt
     1921 gatacgattt tacatcattt ctttgttcta atatttttat ttcatttatt ttctttttta
     1981 taattttatg ataaaataat tagtatattt tgctttactt gaatgtaatt atttatacaa
     2041 aagtcaaaca taatataaat gtagtaaatt tttagtttca agtaatatct cgttattata
     2101 actaaatatt tattaaaatt aggaggataa aatgggctta agagacaaat tcgcgcaatc
     2161 atttgctaga tcaaaaacta tgagtggccc tgagaaaaag gctaatgaaa taatgggtaa
     2221 attgcttctt aagaaagcta tattacctat cgttttaatg ttcgtgataa taatcgctgg
     2281 agctatgctt aaaatcaatt cttgggttac acttggcata aatttagtta ttgctgttgg
     2341 tgctttcttc tacataagaa attctagtaa aaagtatcag aactttaaac cttatgttgg
     2401 caatttgatt agtttagaga aaaaaggtaa aaaagagtac gttgctatta taaaacaagg
     2461 taagttacca gttaagttac aaatagctta tggtggtgaa gaccttgaac atgttaagaa
     2521 aaatcaaatg gttcagataa gttataatcc agatgctaaa atagctattt tggttaatag
     2581 acaataattt aataaaaaaa aggagttgtc ttaaaattga ttttttctaa tcaaatttaa
     2641 gcttaactcc tttttgaa
//
LOCUS       DAMJWP010000319         2607 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.326, whole genome shotgun sequence.
ACCESSION   DAMJWP010000319 DAMJWP010000000
VERSION     DAMJWP010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.326"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(206..1456)
                     /locus_tag="PC700_003505"
     CDS             complement(206..1456)
                     /locus_tag="PC700_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HDF2582768.1"
                     /translation="MATIKPFKAIRPNKYIVDKVAALPYDVMNSKEARRIAEGNPYSF
                     LHIDKSEIDLDENIDLYDEKVYLKARENLYEFRKQEILVKDDKECIYIYKQIMDGRAQ
                     VGIVACISVDESLNGTIKKHEYTRPEKEIDRTKHIKYCDANTGTILVTYKHQRVIDDI
                     INDFMDNNEPLYDFITDDKIEHTVWKIDSDDIIDNLVDKFEKLDYLYIADGHHRTASA
                     ENVAKEMRAKNPNYTGKEEFNYFIAMIAPDENLMVLDYNRVIKDLNGLSEEEFINKIK
                     ENFELEEIEGKEKYKPDKKGTFGMYLGDKWYKMKANKNLLEIEDPVDSLDISILQDYV
                     IDAILGIDNPRVDKRIDFIGGIRGLEELEKRVNEDMKVAFAMYPTSIEDLIRVADANK
                     IMPAKSTWFEPKVRCGLFLHEINE"
     gene            complement(1488..2429)
                     /locus_tag="PC700_003506"
     CDS             complement(1488..2429)
                     /locus_tag="PC700_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HDF2582769.1"
                     /translation="MYNILVTDGIEKEAARKLRELDFNVIEQFYEKDVLGDKLKDVDV
                     LVVRSATKVTKDVIDKALEGKKLKLIVRGGVGLDNIDVKYAQANGIKVMNTPNASSIS
                     VAELTIGQLFVLARFINTANVTMRDGKWEKKKYKGTEINGKTLGLIGFGRIAKEVAKR
                     AELLGMNVIYTDIMGEAQGFNNYKFCDMEEVLENADFLSLHIPFDKNKGAVITEKEIN
                     KMKKGAYLINCARGGLVDEKDLLKALDEGKLSAAAIDVYEQEPTLNLDLVNHPRVSPT
                     PHIGASTVEAQERIGDEIVNVIQDFFLDFNNLIGVAL"
BASE COUNT          784 a          496 c          244 g         1083 t
ORIGIN      
        1 ataaatatac aaatacaatt atatgaattt tcatcctcaa ttatagcata gaatatgact
       61 atctatatcc taaaattatc catttctaaa aaaaggtaac tattattaag aatatcaact
      121 tatagaattt atattccaca tacgattttc aatcaaaaaa acaagggcta aaaagtgaat
      181 tgtacacttt ttagcccttt attaattatt catttatttc atgtaagaat agcccacatc
      241 ttactttagg ttcaaaccat gttgacttag ctggcattat cttatttgca tctgctactc
      301 ttattaaatc ttcaatacta gtaggataca ttgcaaatgc tactttcata tcttcattaa
      361 ctcttttttc taattcttca agacctctaa ttccaccgat aaaatcaatt cttttatcta
      421 ctctaggatt gtcaattcct aaaatagcat caataacata gtcttgtaat atagaaatgt
      481 ctaatgagtc aacagggtct tctatttcta ataaattttt atttgctttc attttatacc
      541 acttatcacc taggtacatt ccgaaagttc cttttttgtc aggtttatat ttttcttttc
      601 cttctatttc ttctagttca aaattttctt ttattttatt tataaattct tcctcgctta
      661 aaccatttaa gtctttgata accctattat aatccaaaac cattaaattc tcatctggtg
      721 caatcattgc tatgaagtaa ttaaattctt ctttgccagt ataatttgga tttttagctc
      781 tcatttcttt ggctacattt tctgctgaag ctgtcctatg atgaccatct gcaatgtata
      841 gataatctaa tttttcaaat ttatctacta ggttatctat tatatcatca ctatcaatct
      901 tccatactgt atgctctatc ttgtcatctg ttatgaaatc atataaaggt tcattattat
      961 ccataaaatc attgattata tcatctatca ccctttgatg tttgtaagtt acaagtatag
     1021 ttcccgtatt agcatcacag tattttatgt gtttagttct atcaatttcc ttttctggtc
     1081 gagtgtactc atgttttttt attgttccat tcaagctttc atcaactgat atacatgcta
     1141 ctataccaac ttgagctcta ccatccatga tttgtttata tatatatata cactctttgt
     1201 catcttttac tagaatttct tgttttcgaa actcatataa attttctcgc gcttttaaat
     1261 aaactttttc atcatataaa tctatatttt catctaaatc aatttctgat ttatctatat
     1321 gcaaaaatga atatggattc ccttctgcaa ttcttctagc ttctttacta ttcataacat
     1381 cgtaaggaag tgctgctact ttatctacta tatatttatt tggtctaatc gccttaaatg
     1441 gtttaatcgt tgccattact aatttcctcc taattataca aaatatttta taatgctacc
     1501 cctattagat tgttgaagtc aagaaagaaa tcttgaatca catttactat ttcgtctcct
     1561 attctttctt gagcttctac tgtagatgcc ccaatatgag gtgtaggaga aactcttgga
     1621 tgatttacta agtctaaatt taatgtaggt tcttgttcat aaacatctat agcagctgct
     1681 gataatttac cttcatctaa tgcttttagt aaatcttttt catcaactaa accgcctctt
     1741 gcacagttta ttaaataagc ccctttcttc atcttattta tttctttttc agttattact
     1801 gcccccttat tcttatcaaa tggtatgtgt aaagataaga aatctgcatt ttctaaaact
     1861 tcttccatat cacagaattt ataattgtta aaaccttgcg cctcacccat tatgtcagta
     1921 tatataacat tcatacctaa taattctgct ctctttgcaa cttcttttgc aattcttcca
     1981 aaacctataa gtccaagtgt ctttccatta atttctgtac ctttatactt cttcttctcc
     2041 cattttccat ctctcatagt tacatttgca gtatttatga atcttgcaag aacaaataat
     2101 tgacctattg taagctctgc aacagatata ctgcttgcat ttggagtatt cataactttt
     2161 atgccattag cttgagcata tttaacatct atattatcca gacctactcc acctcttact
     2221 atcaacttca actttttacc ctctaatgct ttatctatta catctttagt aactttagta
     2281 gctgaacgta ctacaagtac atcaacatct ttaagtttat ctcctaaaac atctttttca
     2341 taaaactgct ctatcacatt aaaatctaac tctctcaatt ttctcgctgc ttctttctct
     2401 attccatcag taactaatat attatacata acaaactcct ccaattttta ttcacttaat
     2461 ttttccaatt tttatttttt aaagtatttt catcaataaa aaactttact taatttaact
     2521 ttctgtttat ataattttca aaatattttt ttattaaagt catatcaaat tttcctattg
     2581 tttgttaaaa atttaatatg taatttt
//
LOCUS       DAMJWP010000320         2581 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.327, whole genome shotgun sequence.
ACCESSION   DAMJWP010000320 DAMJWP010000000
VERSION     DAMJWP010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.327"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            306..1925
                     /locus_tag="PC700_003507"
     CDS             306..1925
                     /locus_tag="PC700_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HDF2582770.1"
                     /translation="MNIVISLIGGLGLFLYGMSLMGEGLQKSAGDKLKKIIELLTSNV
                     VMGVLVGTVVTGIIQSSSATTVMVVGFVNAGIMNLSQAIGVIMGANIGTTVTAQLVSF
                     NLEGIAPIALGIGILFYLFTSNQKTKHLSEILIGFGILFTGMEFMKDAVAPLAEYKAF
                     TDALLYFSKNPVLGILAGFAITGIVQSSSASMGMLIALASQGILPLSSALPILYGDNI
                     GTCVTSLLSSVGASRNARRAAVMHLSFNVIGTIIFMLVLNKPISAIVTHFDPTDTARQ
                     IANAHTLFNLTNVIILLPFSKYIVKLANRLIPIKETESEIVNNTKYLDERMFSTPSIA
                     LGNTVQEVVRMGHKATNSLEHSIAGFLNKSNEDINKAFESEKVVNKLQKDILNYLLKL
                     SKEPLRDDERFRTDLLFNTVNDIERVSDHAENIAELAMSVKEMNISFSDSAIREIYEI
                     YNKTITNFKDALIVLDVKDFELANKVLEVENEVNYLEKTFRNSHMIRLNNGNCTIDAG
                     VLYLDLLTNLERISDHSTNIVKQVLKLKQKI"
     gene            2299..2493
                     /locus_tag="PC700_003508"
     CDS             2299..2493
                     /locus_tag="PC700_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2582771.1"
                     /translation="MINYMVEIIYSEKISEVENMLKIENIQYEFTSRDGFIFFGFYEM
                     TDAYRFETLCSDEKIRFKRI"
BASE COUNT          959 a          300 c          375 g          947 t
ORIGIN      
        1 atatcattct aaaaggatgc tattttttta catatttttt acaattatag ttaattcttt
       61 gttaaattga ttgtacataa aatactaaaa atctaagttc aagtatttaa attgctatat
      121 atcataaatt ttctaaaatt aacaacaact taaaaattta acattctctt aacgtatact
      181 taacaaaaca ttaatatttc tattgacaaa atttgttatt ctatattatg gaaacaataa
      241 attttagtta ataaagttta taattcagat aaaaaaacta ttaatttctt aagggaggaa
      301 atatcgtgaa tatagtaatt agccttatcg gtggacttgg actttttctt tacggaatga
      361 gtttaatggg agaaggttta caaaaatcag caggagacaa attaaaaaaa attattgaac
      421 tcttaacaag taatgttgtt atgggtgtac tggtaggtac agtcgttaca ggtattatac
      481 aaagttcaag tgctactact gttatggttg taggatttgt aaatgcaggt ataatgaatc
      541 taagtcaagc tattggtgta ataatggggg caaatatagg tactacagtt actgctcaat
      601 tagtttcatt taatcttgaa ggaattgcac caattgcttt aggtataggt attttattct
      661 atttatttac atcaaatcaa aaaacaaagc atttatctga gatacttata ggttttggta
      721 tattatttac aggaatggag tttatgaaag atgcagtagc tccacttgct gaatataaag
      781 cattcactga tgcactttta tattttagta aaaatcctgt attaggtata ttggcaggat
      841 ttgccataac tggtattgtt caaagttcaa gtgcatctat gggtatgtta atagcactag
      901 cttctcaagg aatacttcct ctatcatctg ctttacctat tctttatgga gataatatag
      961 gtacttgtgt tacttcactt ttatcaagtg ttggtgcaag tagaaatgct cgacgtgcag
     1021 ctgtaatgca tttaagtttt aatgtcattg gtacaataat atttatgctt gttttaaata
     1081 aacctatatc agccatagtt actcattttg acccaactga tacagctaga caaatagcaa
     1141 atgcacatac tttatttaat cttacaaatg ttataatatt attaccattt tctaaataca
     1201 tagttaaatt agcaaataga cttataccta ttaaagaaac tgaatcagaa atagtaaata
     1261 atactaagta cttagatgaa aggatgttta gcactccatc tatagcactt ggtaatactg
     1321 tacaagaagt tgtaagaatg ggtcataaag ctacaaactc tttggaacat tcaattgcag
     1381 gatttttaaa taagagcaat gaagatatta ataaagcttt tgagtcagaa aaagttgtca
     1441 ataagttaca gaaagatata ttgaactatt tgttaaaact ttctaaagaa cctcttagag
     1501 acgatgaacg ctttagaaca gatttgcttt ttaatactgt aaatgacatt gaaagagtct
     1561 ctgaccatgc agaaaatata gctgaattgg ccatgagtgt taaggaaatg aacattagtt
     1621 tttctgatag tgcgataaga gaaatatacg aaatatacaa taagacaatt actaatttta
     1681 aggatgcctt aatagtttta gatgttaaag attttgagtt agcaaataaa gttttagaag
     1741 ttgaaaatga agtaaattat ttagaaaaaa cttttagaaa ttcacatatg attagactaa
     1801 ataatggaaa ttgtacaatt gatgctggtg tactttatct tgacttactt actaatctag
     1861 aaagaatttc tgaccactca acaaatatag taaaacaagt attaaaactt aaacaaaaaa
     1921 tatagtgatt atttttatat ataatcttaa tcaatgagaa gtaagaagct atagataatg
     1981 cttatatcat ttatggcttc ttatttttta tgtaatactc aaatttttat agtattctct
     2041 tattattaaa cagcttctgc ctagaatatt ccatatatat atgacttctt tcataaaact
     2101 tgaactgccc ataactaaag tcacaagtat acataactat tttttaatca attataacca
     2161 atatttattt tatttatctt cattttaatt cttcattcaa taatgtaata agattatttt
     2221 ttatttggat actattatat catctaaata tgttattata tatttggata ttttcatatt
     2281 aataggaggg attctacaat gataaattat atggtagaaa ttatatatag cgaaaaaata
     2341 agcgaagtag aaaacatgtt gaaaattgaa aatatacaat atgaatttac ttctcgtgat
     2401 ggctttatat tttttggttt ttatgagatg acagatgcat atagatttga gactttatgt
     2461 tcagatgaaa aaattcgatt taaaagaata taataaataa aaaaagtaca tatttctaaa
     2521 ctatttgttt ttcaaattta ggttgtgttt taatgtagtg ttgatatcat aaacaatttt
     2581 a
//
LOCUS       DAMJWP010000321         2561 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.328, whole genome shotgun sequence.
ACCESSION   DAMJWP010000321 DAMJWP010000000
VERSION     DAMJWP010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.328"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            459..938
                     /locus_tag="PC700_003509"
     CDS             459..938
                     /locus_tag="PC700_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2582772.1"
                     /translation="MDKTTFTNNILESEQTLYRVSKSILGNDQDCEDAVNNAILKAYE
                     KLDSLKEEQYFKTWLIRIVINECNSLRRKRLKSLSFEDVFKNKKIDEKDDYSDLYTAI
                     QSLPKKIKIPIVLYYIEGYSVDEVKEILDIPQGTVKSRLSRGRTLLKTKLENTEVII"
     gene            935..1714
                     /locus_tag="PC700_003510"
     CDS             935..1714
                     /locus_tag="PC700_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582773.1"
                     /translation="MNKNILNNSFPEVPESFHNSLSKTLNSLPEREENYKMINNKTHK
                     LPFRKGLVATLAITLILSTTALAVGHIYSLVGKSSTKATYTSIPTTEQVKKDFNFTPN
                     IVSEFSNGYKFKGAYTVNKKALDKENNVLGKSKALSFEYKNGNDEISLDTANNVLEKS
                     SDNLKVVDTYKDINISYEAFAQKFVPEGYKMTEQDKIDEKSGKYTFSVGPNNESDKVE
                     VNEFKFLTWKQDGVYYSFTVQDSNLSMNDLVKMAHEVIDAK"
     gene            complement(1964..2464)
                     /locus_tag="PC700_003511"
     CDS             complement(1964..2464)
                     /locus_tag="PC700_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2582774.1"
                     /translation="MIEGIKFIKAEEKYIYSYWQTFDKIAKERKYLAMDEAFPFEETV
                     EFIKNTINKNLPQLFIIDLESDNCIGWCDVLPKTEKVGYLGMGILKEYREKGIGSSLL
                     KQIIDLSKEYGYEKIELDVFKSNSRAIHVYKSLGFVEVNTISSGFTWNDRPVKEEVIQ
                     MELTLI"
BASE COUNT         1009 a          350 c          314 g          888 t
ORIGIN      
        1 tctatctaaa ataatgacat ttctaactct tgattctcat ttaagtttca ttgttttttc
       61 ttaaaagcct acttttagtc aaaataccgt tatctttagt cattaattaa aaaacttatt
      121 ttaaatctat acatgaagta aataatccaa aagcattatt ttctaactac tcaacatcaa
      181 atacttacta ttttataatt acttaatata aacacttact ataaaactaa atatataaat
      241 ggcttctaaa gtttaatata tctacatggc tttatatatg aggggaaaat actagaaaaa
      301 aatatattat agaatatttg tatagaatag gcaaaacttt caaacttaaa tttatcaaat
      361 aatcataaaa aaagtgaacc atctatagta aaattttatc taataagtga aaggcaaaaa
      421 agtagcgcta cgatttgcat agaagggaag gaactcagtt ggataaaact acatttacaa
      481 acaatatact tgaatcagaa cagactttgt atcgagtatc taagtctatt ctaggaaacg
      541 accaagactg tgaagatgcc gtcaacaatg ctattttaaa agcatatgaa aagttggatt
      601 ctcttaagga agaacaatac tttaaaacat ggctgattag aatagtcata aatgaatgta
      661 attcattaag acgcaaacga ttaaaatctc tatcatttga agatgtcttc aaaaacaaaa
      721 aaattgatga aaaagatgac tatagcgatt tgtatactgc tattcaaagt ctacctaaaa
      781 aaattaaaat tccaatagtc ctttactata ttgaaggtta ttcagtagat gaggttaagg
      841 aaatattaga tatacctcaa ggaactgtaa aaagtagatt atcaagagga agaacattat
      901 taaaaactaa attagaaaac acggaggtaa tcatatgaat aaaaatatat taaataattc
      961 gtttccagaa gttccagaaa gttttcataa tagtttaagt aaaacactaa atagtttacc
     1021 agaaagagag gaaaattata aaatgattaa taataaaaca cacaaattac catttagaaa
     1081 aggcttagtt gcaactttag ccattacttt gatattgagc acaacagccc ttgcagtagg
     1141 acatatatat agtttagttg gaaaatcaag tactaaggct acctatacat ctatcccaac
     1201 tacagaacaa gtaaaaaaag actttaactt tacccctaat atagtttctg aatttagtaa
     1261 tggatataaa tttaaaggag catatactgt aaataaaaaa gcacttgaca aagaaaataa
     1321 tgttttagga aaatcaaaag cacttagttt tgaatataaa aatggcaatg atgaaatatc
     1381 tcttgacaca gcaaataatg tacttgagaa gagttctgac aatctaaaag ttgtagatac
     1441 ttacaaagat attaatattt cttatgaagc attcgcacaa aagtttgtac cagaaggtta
     1501 taaaatgaca gaacaggata aaattgatga aaaatcaggc aaatacacat ttagtgtagg
     1561 gccaaataat gaatcagaca aagttgaagt taatgaattt aagtttttga catggaaaca
     1621 agatggagtt tactattctt ttacagttca agactcaaat ttatctatga atgacttagt
     1681 taaaatggct catgaagtaa tagatgcaaa ataaacataa gtaataataa gttgtttatg
     1741 agtttattat ttatgacttt aattttttta ttagtaaaca tggttgtatc ttccatcttt
     1801 atattttctt taaatttaat atataaaatt ttagatattt agctaccttc aaaagaacca
     1861 ttattaaaaa gcccttaaaa atatttcttt tatatggaat tttaagggct ttaactttat
     1921 tttatacaag tgtatatgga attttaaaga ctttaacttt attttatata agtgtaagct
     1981 ccatttgaat tacttcttct tttacaggtc tatcattcca tgtaaaacca ctagatattg
     2041 tatttacctc tacaaaccct aaagatttat aaacatgaat agctctacta ttacttttaa
     2101 atacatcaag ctcaattttt tcatacccat attcttttga taaatcaata atttgtttta
     2161 aaagactact tccgatacct ttttccctat actctttaag aatacccatg cctaaataac
     2221 caactttttc agttttaggt aaaacgtcac accaaccaat acagttatct gattctaaat
     2281 caattataaa cagttgtggt aagtttttat ttatagtatt ttttataaat tcaactgttt
     2341 cttcaaaagg aaaggcttca tccatagcta gatatttcct ctcttttgct attttatcaa
     2401 aagtttgcca atacgaataa atatactttt cttctgcctt tataaatttt attccttcta
     2461 tcaacttaac atttctcctt tttaatttat gattgtagtt aacttatttt atatgattct
     2521 agtttataat gctatataaa ttactatata ttagcttaaa a
//
LOCUS       DAMJWP010000322         2522 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.329, whole genome shotgun sequence.
ACCESSION   DAMJWP010000322 DAMJWP010000000
VERSION     DAMJWP010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.329"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            50..850
                     /locus_tag="PC700_003512"
     CDS             50..850
                     /locus_tag="PC700_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDF2582775.1"
                     /translation="MTFKNKKFTINKYEILSIILVLCLILTGFSSYVNYSHKKFKDYV
                     VFYVQHQDDEVLWAGSAIVNAIKERGKNHVFVVLVSTGCGISVFDKYKKYENLTNIQK
                     SEYRNREFLASLNSLGVIRENIILLPETNTNGNTDFNYMEKIALTFEEKFKNVTHIAH
                     TYKLDDHLQHLKNGSVIQNLYNVGKIKDAKYFVKPKFADKITFNNKIVYKAHSTEDYE
                     KVKNACQQYKIVDELEYREGIGYKSDHKSFDKLLNNKDVPAILHTPNL"
     gene            1391..2107
                     /locus_tag="PC700_003513"
     CDS             1391..2107
                     /locus_tag="PC700_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2582776.1"
                     /translation="MNQNILVVDDDREIVDAIEFYLRPDNFNVLKAYDGLEALDILIE
                     NDIKLIIMDVMMPNLDGLKTTLKIREKRNIPIILLSAKSEDMDKIIGLNMGADDYITK
                     PFNPLELTARVKSQLRRYINLGDFSNTNNSNDTNYILKSGGLELNTDTKIVSLDGEEI
                     KMTPLEYKILTLLMSRKGKIFSSREIYENVWNEDAYSCERTIAVHIRRIREKIEINPK
                     EPKYLKVVWGIGYKIEKLNY"
BASE COUNT          983 a          285 c          339 g          915 t
ORIGIN      
        1 gtactttgtt tgataatata actaagacaa cttttcagga gatgattaaa tgacatttaa
       61 gaataaaaaa tttactataa ataaatatga aattctatct attattctag ttttatgctt
      121 gattttaacc ggttttagta gctatgtgaa ttatagtcat aaaaaattta aagattatgt
      181 tgtcttctat gttcagcatc aagatgatga agtgttgtgg gctgggagtg ctattgttaa
      241 tgctataaaa gaaagaggaa aaaatcatgt atttgtagtt ttagtatcta ctggatgtgg
      301 tatatccgtc tttgataaat ataaaaaata tgaaaaccta acaaatatac aaaaatcaga
      361 atatagaaat agagaatttt tagcttctct taatagcctt ggtgtaataa gggaaaatat
      421 aatattatta ccagaaacaa atacaaatgg taatactgac ttcaattaca tggaaaaaat
      481 tgctcttaca tttgaagaaa aatttaaaaa tgtaactcat atagcacata cttataagtt
      541 agatgaccat ttacaacatt taaaaaatgg ttctgttatc caaaatttat ataatgttgg
      601 taaaataaaa gatgctaaat attttgtaaa acctaaattt gctgataaaa taacttttaa
      661 taataagata gtttataaag ctcattctac tgaagactat gaaaaggtta aaaatgcctg
      721 tcaacaatac aaaattgttg atgaattaga atatagagaa ggtattggct ataagtctga
      781 ccataaatca tttgataagt tattaaataa taaagatgtt cctgctatac ttcatacacc
      841 taatttataa gaaatattta ttcttttaat ttaagttata agaaggaaat tatttgagat
      901 tttgtgatat agcacttttt gtgctatact actttttctc attcaactat tacccatatc
      961 taatttcatc atctcttaaa atatataaag atatcatcat taaaacctat aagccttatt
     1021 aaatacttta tacatatcta cattaaacta cctatttata ttaaactaca ttaaaataaa
     1081 tatttgttat aaattcttaa taattatgaa actcatttgt tatttatatt tgttaatata
     1141 tgtttaaacc taaaaaatta ataaacccaa aataacataa ggagtgtgat agattaccct
     1201 agatattttc ataaactttt accttcagat taatatgggc gtctatactt ttaaatttac
     1261 tatatgcatt gacaatatat taaaagtact agtttttatt tcataatgaa cgggcttact
     1321 gttttctaat acctcttttt ggtataaaat aaaaattggt taaaaataaa atttcaaggg
     1381 ggttaataaa ttgaatcaaa atatattggt tgtagatgat gatagagaga tagttgatgc
     1441 tatagagttc tatttgagac ctgataactt taatgtttta aaggcatatg atggacttga
     1501 agcattggac atactaatcg aaaatgatat taagttaata ataatggatg taatgatgcc
     1561 caatttagat gggcttaaaa ctacacttaa aataagagaa aaaaggaata taccaataat
     1621 tctcttatct gcaaaatcag aagatatgga caaaattata ggtcttaata tgggtgctga
     1681 tgactatata actaaaccat ttaatccact agaacttact gctcgagtaa aatctcaact
     1741 tagaaggtat ataaatttag gtgattttag taatacaaat aactcaaatg atactaatta
     1801 tattctaaaa agtggtggcc tagagctaaa tacagataca aaaatagttt ctttagatgg
     1861 tgaggaaatt aaaatgacac cacttgaata taaaatactt acacttctta tgtcacgaaa
     1921 aggtaaaatt ttctcttccc gtgaaattta tgaaaatgtc tggaatgaag atgcttatag
     1981 ttgtgagaga actattgctg ttcatattag aagaattaga gaaaaaattg aaattaatcc
     2041 aaaagaacca aaatatttaa aggtggtgtg gggcattggt tacaaaattg aaaaacttaa
     2101 ttattaatga ttctaaagca aaatttattt tattattatt actgtttcta ttagtttcta
     2161 tattatttga agcagtttat gatttgaata ataacttttc ttataatatt aattgttatt
     2221 ataaagagtt tgctctccct tatttttatt ttttcggtac tcctgtacat ttgtcaaaag
     2281 tagaattaga gtttgtctgc ataggtatta tattatcaag ttttttattg actatactat
     2341 gttttacatt gtataaaaaa aataaacaga ctaaagtcaa attcttagag aatgtcataa
     2401 gccttttttc taaggtaaaa atagaactta cacttatatt aactttgata ctattaacct
     2461 taaggtttca tgcatttaca agtaataaaa tgttttacaa cactaattta ataatctaca
     2521 at
//
LOCUS       DAMJWP010000323         2518 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.330, whole genome shotgun sequence.
ACCESSION   DAMJWP010000323 DAMJWP010000000
VERSION     DAMJWP010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.330"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(181..2370)
                     /locus_tag="PC700_003514"
     CDS             complement(181..2370)
                     /locus_tag="PC700_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HDF2582777.1"
                     /translation="MYEVNLERAMNYWMDLLDDYTNIAKIPNDFFYKDCSDEVVDEVF
                     KMNIEKHERLIQYSKVNNISIDSIIETIYGLILQKYIYEDDVVFGKTMNIIPIRVRVR
                     QSEKFIDVVRKLKRQWSRSLEYGYYLLSDIENKNHLNTKLINTIFIAKSFDLSYDYDI
                     EKKGYDLSVIVYTKRALKIKIKYKPSIYKRETIRRILNQFDYIIEQIIEDNDILIEDL
                     EFVCKEEEKVLLEDFNNNQYEVPYDKSYVDLFREQVKVTPSNIAARDENRKLTYKELD
                     EVTDKLAGYLNHIGVESEDIVAVMLPRDINIIITAIGIMKSGGAFFPIDTSNPEERLN
                     YLLEDSNAKVVITTDELKSKVVNENTIVLDINDEEMFKLGYELTEKITSSNCAYTIST
                     SGSTGRPKTIAIEHKSLVNMCYYSVKSISATENDICGIYLSFSFDAVMKQLFPYLLVG
                     ASIDIMPEEAKFDEYTVNEYCEENDITILALPTAFARLFIQNCNNNSLRVVQTGGERL
                     KGYRKRNYELYNEYGPTEFTVVSTSFHVDREYGKIPIGKPIFNTYAYVLDKKNKLCPI
                     GAPGELCLSGIQISRGYLNKKGLTEQVFVENPYKTCEYNKLMYRTGDLVRWLDDGNLD
                     YIGRMDNQVKIDEFRIELYEIENIINNITEIKSVVCISRTNDDGDMYICAYYVIDEED
                     SGKINERTIREYLNEHLPPYMIPTIIMRIDKIPVTPIGKVNKKALPE"
BASE COUNT          803 a          455 c          240 g         1020 t
ORIGIN      
        1 aaaaatgctt taaaacacgg aaaagtataa aaaatacaat aatcattctt ataaataaat
       61 tttcaccata gtaaatatat acagaaaatt ctttttttta catcaattga tttcctcaga
      121 aaaataggtg tcataaagaa atgtatggaa tcactttaca acacctgtca taataaaaaa
      181 tcactctggt agtgcttttt tatttacttt tcctattggt gtaactggta ttttatctat
      241 cctcattatt attgttggaa tcatatatgg aggtagatgc tcatttaaat attctctaat
      301 ggttctttca tttatcttac cactatcttc ctcatcaatt acataatatg cacatatata
      361 catatccccg tcatcatttg ttctcgatat acaaacaaca cttttaattt ctgtaatatt
      421 atttattata ttttcaatct catatagctc tatcctaaac tcatcaattt ttacttgatt
      481 gtccatcctt ccaatataat ctaaatttcc atcatctaac catcttacta aatcaccagt
      541 tctatacatt aacttattat attcacaagt tttgtaagga ttctctacaa atacttgctc
      601 tgttagccct tttttattta aatatcctct tgatatttga atgcctgata gacatagctc
      661 tcctggtgct ccaattggac atagtttgtt ctttttatct aatacatatg cataagtatt
      721 aaatataggt tttcctattg gtatttttcc atattctctg tccacatgaa aagaagtcga
      781 tacaacagta aattcggttg gtccatactc attatatagt tcataattac gctttctata
      841 tccttttaat ctttctcctc cagtctgtac aactctaagt gaattattat tgcaattttg
      901 tataaataac cttgcaaatg ctgttggcaa tgctagtata gttatgtcat tttcctcaca
      961 gtattcattt actgtatatt catcaaattt agcttcttca ggcataatat ctattgatgc
     1021 acctacaagc aaataaggga atagttgttt cattacagca tcaaaactga aacttaagta
     1081 tataccacat atatcatttt ctgtagcgct tatagatttt acgctataat aacacatatt
     1141 tactaaactc ttatgttcta tagctatggt ctttggccta ccagttgacc ctgaagtaga
     1201 tatcgtatat gcacaatttg atgatgttat tttttcagtt aattcatatc caagcttaaa
     1261 catttcttca tcatttatat cgagtactat ggtattctca tttacaacct tacttttgag
     1321 ttcatctgta gttatcacta cttttgcatt actatcttca agaaggtaat tcaatctttc
     1381 ttctggatta ctcgtgtcta ttggaaaaaa tgctccccct gatttcataa tccctattgc
     1441 tgtaataatt atgttaatat ctcttggtaa cattacagct actatgtctt cactttccac
     1501 tcctatatga tttaaataac ctgcaagttt atcagttacc tcatccaatt ctttatatgt
     1561 taactttcta ttttcatccc tagcagctat attactaggt gttaccttaa cttgctccct
     1621 aaataaatca acataacttt tatcataggg tacttcatat tgattattat taaagtcttc
     1681 caataatact ttttcttctt ccttacatac aaattctaaa tcttctatta aaatatcatt
     1741 atcttctatt atttgctcta ttatataatc aaattgattt aaaattcttc taatagtttc
     1801 tcttttatat atagatggtt tatactttat ttttatttta agtgccctct tagtatatac
     1861 tattacagat aaatcatatc cttttttttc tatgtcataa tcatatgata aatcaaaact
     1921 tttagctata aatattgtgt ttataagctt agtatttaga tgatttttat tctctatatc
     1981 tgataacaaa tagtatccat actctaaaga cctgctccac tgccttttta gcttccttac
     2041 aacatctata aatttttcac tttgtctaac tcttactctt attggaatta tattcattgt
     2101 tttaccaaaa actacatcat cttcatatat atacttttga agtataaggc catatatagt
     2161 ttctatgata gagtctatgc taatattgtt cactttagaa tattgaatta acctttcatg
     2221 tttctcaata ttcatcttaa aaacctcatc tactacttca tcagaacaat ctttataaaa
     2281 aaaatcattt ggaatttttg ctatattcgt ataatcatct aacaaatcca tccaataatt
     2341 catagctcgt tctaaattaa cttcatacat atcaaaattc ccccttacta ctaagtctat
     2401 agaaaaaatt cttttggaat tttattttca caatatataa atatattaat aatacatatg
     2461 caagctctaa aaacatgagc ttgcataaaa ttacacttat tatgactact aaattcgt
//
LOCUS       DAMJWP010000324         2500 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.331, whole genome shotgun sequence.
ACCESSION   DAMJWP010000324 DAMJWP010000000
VERSION     DAMJWP010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.331"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            85..2103
                     /locus_tag="PC700_003515"
     CDS             85..2103
                     /locus_tag="PC700_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2582778.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIVLGSKSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTESYEGTINYNKDPE
                     YLNVWELQGITINSKNNPKTLNKQELEKMGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSAPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPRDVIIIGDENAISKTTANQIKSTVNASQTRLKGSNRY
                     ETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKNSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNDITKDNVTNNRVYGADRHETNANVIKKFY
                     TDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKDNLEAKSANKVY
                     KIGGGLTSKVMNSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSDTGTTGKPLGGIK
                     EKDYTLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLRPDLFTSIHYNAS
                     DTTGNGVEVFYKLKDKDGGTTKTVATNILNRILEKFNLKNRGAKTRTLSTDPTKDYLY
                     VLRNNDMPAVLVECAFLDNEKDMSLLNTSNKVKEMGTQIGKGIEDSLK"
BASE COUNT         1047 a          294 c          422 g          737 t
ORIGIN      
        1 ttgggtaaaa acactttgat ataatttcaa aaattaaaag gttaaaaaac aaagtagaaa
       61 tttatgttaa ataaggagat taatatgaga agaaatacaa aattattaac aacagggatt
      121 ctttcaatgg caatcgtcgc acctacaatg gcatttgcta ctgaatctaa tgctatggaa
      181 aataacgctg atttaaatat aaacttagag aaaaaaagta tcgttttagg tagcaaatca
      241 aaagtcagtg tcaagtttaa agaaaaacca gatgcagata gcattacatt aaagtataaa
      301 tgctatgata tgccattgga tacaactcta aattataatc aatcaactga gtcatatgaa
      361 ggaactatca attataataa agacccagaa tatttaaatg tttgggaact acaagggata
      421 acaataaaca gcaaaaataa tcctaaaact ttaaacaaac aagaactaga aaagatggga
      481 ttaaatttaa aagactataa tgtaacgcag gaatgtataa ttgaagatat aacttctaga
      541 aaagatgtaa ataaatattt aagaaaaact tctgcaccta ttacagaact tacaggaagt
      601 gatagatacg aaacagcagt taaaataagt aaagaaggct ggaaaaatgg ttcagataag
      661 gtagttataa taaatgggga tgtaagtata gatggcatta tatcaactcc actagcaact
      721 acatataatg ctccaatact tttggttgaa aaaaataatg tacctaatag tgtaaaatca
      781 gaattaaagc gtttaaatcc tagagatgta attataattg gagatgagaa tgctatttct
      841 aaaactactg ctaatcaaat taaatcgact gtaaatgcta gtcaaacacg tctaaagggc
      901 tctaatagat atgagacatc tttattgata gcaaaggaaa tagataaaaa tcatgatgtg
      961 gaaaaagtat acataacaaa tgctaatggc ggagaagtgg atgcacttac tatagcagca
     1021 aaagcaggtc aagacaagca accaattata ttaactgata aaaatagtat tacagacaac
     1081 acatataaat ggttaaagag tgaggattta caaaatgctt attttatagg tggacctcaa
     1141 atgatatcaa caaatgttat aaataaggta aatgatataa ctaaagataa tgttactaat
     1201 aatagagtat atggagcaga tagacacgaa acaaatgcaa acgtaataaa aaaattctat
     1261 acagatgatg agttagaggc tgttttagta gctaaatcag atgtacttgt tgatgcttta
     1321 gcagcaggtc cattggctgc gaacttaaaa tctccaatac ttataacacc aaagacgtat
     1381 gtatctgcat accataaaga taatttagaa gctaaatcag ctaataaagt atataaaata
     1441 ggaggaggat tgacttctaa ggtaatgaac tctatagcat catcattatc taaacacaat
     1501 acaactccaa cagaaccagg aaatagtggg ggtaagacag ttatgattga cccaggacat
     1561 ggtggttcgg acactggaac aacaggtaaa ccattagggg gtattaaaga aaaagactat
     1621 acattaaata cttcacttgc gacaactgaa tatttacgtt caaagggatt caatgtaata
     1681 atgacaagag acacagataa gactttatct cttggaaata gaactgcttt atctaactct
     1741 ttgagaccag atttatttac gagtatacac tataatgcat ctgatacaac tgggaatggt
     1801 gtagaagtat tttataagct taaagataaa gatggaggga ctactaaaac tgtagctaca
     1861 aatatattaa atagaatctt agagaaattt aatctaaaaa atagaggtgc aaaaacaaga
     1921 acactatcaa ctgaccctac aaaagattat ttatatgttt taagaaataa tgatatgcca
     1981 gctgtacttg tagaatgtgc atttttggat aatgagaaag atatgagttt acttaataca
     2041 tcaaataaag taaaagaaat gggtacacaa ataggtaaag gcatagaaga ttcattaaaa
     2101 tagtctacaa tgagagcata gaaaaattta aaataaatta atctgcaatt ttaaatttaa
     2161 actaaagcta taagataaga atgtaattat aatagtaata aagactgtat atacattgtg
     2221 catataagtg cgaaatgagg tatatacagt ctttttatga ttaattgtaa aaggatttat
     2281 attatatgaa gaatttacat atcttaaggc tacagttaat tgtcaagagt tggagtattc
     2341 atttgtaata cacaataaaa gcttaattac tatattttat tgtgtgtttg tgtgtttgtg
     2401 tgttttttgt ggatttttat gtcaataaat gatatagtat agataattaa atgtattaaa
     2461 tatatttaat ttttttattt tttgagtttt ataagtttat
//
LOCUS       DAMJWP010000325         2455 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.332, whole genome shotgun sequence.
ACCESSION   DAMJWP010000325 DAMJWP010000000
VERSION     DAMJWP010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.332"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            49..783
                     /locus_tag="PC700_003516"
     CDS             49..783
                     /locus_tag="PC700_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2582779.1"
                     /translation="MKQNKYDDDRFFNKYSKMERSTNGLAGAGEWHVLKKMLPDFKGK
                     RVLDLGCGFGWHCQYAVENGAISAVGVDISEKMLKEARNKTQLDNINYICMPIEDINF
                     PKDSFDVVISSLAFHYIQSFEDICKNISNCLSNKGNFVFSVEHPIFTAYGSQEWYCDK
                     DGNNLHWPVDRYFEEGVRKSNFLGEEVIKYHKTLTTYLNTLMKTGFEILEIIEPQPEE
                     KLLDTIPDMKDELRRPMMLLVSAKKR"
     gene            complement(925..1416)
                     /locus_tag="PC700_003517"
     CDS             complement(925..1416)
                     /locus_tag="PC700_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDF2582780.1"
                     /translation="MNNQQQEVYKKLDELSIPYEAVNHPPVYTIEEMEELKTLHMEFV
                     VKNLFLRDAKGKEHFLVVLGKDKKADLKDIRQQIDSKPLSFASEERLQKYLKLTKGAV
                     TPFGILNDKEAVVKVVFDKDLLKMDKIGIHPNDNTATVFLKFEDMKKLIEENGNEIYY
                     VEV"
     gene            1765..2148
                     /locus_tag="PC700_003518"
     CDS             1765..2148
                     /locus_tag="PC700_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582781.1"
                     /translation="MSTDKVVGIIDEETAELAGIEYTGKIYASSGVIKHIKKKHRCQL
                     SKDIFNDIIDTIKVVLKSPEYIGSHPKKPGKSVEFIKKIDNYILVATELDTKKGYLYV
                     SSMYAIKEAKLESRVNNGRVKQYDG"
BASE COUNT          956 a          276 c          359 g          864 t
ORIGIN      
        1 tttaatagag tgttgctaag tgaatatatt taaaatggag gaaactttat gaaacaaaat
       61 aaatatgatg atgatagatt ttttaataaa tacagtaaaa tggaacgttc tacaaatgga
      121 ttagcaggtg ctggagaatg gcatgtttta aaaaaaatgt tacctgattt caaaggaaaa
      181 agagttttag atttaggatg tggatttgga tggcattgtc aatatgcagt tgagaatgga
      241 gcaatttctg ctgttggagt tgatatttca gaaaaaatgt taaaagaagc tagaaacaaa
      301 acgcaacttg ataatataaa ttacatatgt atgcctattg aagatattaa ttttccaaaa
      361 gatagttttg atgtagttat tagttccttg gcatttcatt atattcaatc atttgaagat
      421 atatgtaaga atataagtaa ttgtctttca aataaaggta attttgtatt ttctgttgaa
      481 catcctatat ttactgcata tggaagtcaa gagtggtact gtgataaaga tggcaataat
      541 ctacactggc cagtagatag atattttgaa gaaggagtac gtaagtcaaa ttttctagga
      601 gaagaggtta taaaatatca taaaacatta acaacttatt taaatacgtt aatgaaaaca
      661 ggatttgaaa tattagaaat tattgaacct caaccagaag aaaaactgtt agatacaatt
      721 cctgatatga aagatgagct acgtagacct atgatgttac ttgtatctgc aaaaaaaaga
      781 taagtaaata taaataatca ttattgtaga aaaaaattgt agaaaataat tacactaatg
      841 gagatgatta taccagtggt attaattaca ccagtagtaa ggaggcaaaa gcattcgttg
      901 cttttgccat tttttaagtt gcaattatac ttctacgtag tatatttcgt ttccattttc
      961 ttctataagc tttttcatat cttcaaattt taaaaataca gtagcagtat tatcatttgg
     1021 atgtatacct attttatcca tttttaataa atccttatca aatacaactt ttacaacagc
     1081 ctctttatca tttaatatac caaaaggagt cacagctcct ttagtaagtt tcaagtattt
     1141 ttgaagtctt tcttctgatg caaaactaag aggtttacta tcaatttgtt gacgaatatc
     1201 ttttaaatct gcttttttgt ctttacctaa tacaactaaa aaatgttctt ttcctttagc
     1261 atcacgaaga aataggtttt ttacaacaaa ttccatatgt aatgttttta attcttccat
     1321 ttcttcaatt gtataaactg gtggatgatt tacagcctca taaggtatgc ttaactcatc
     1381 taatttttta tatacttcct gttgttgatt gttcatgatt tcctcccatt agaaatttat
     1441 tttatatata aataatatta tcacagaaaa ctttaaaaga aaacgtttta tataatatag
     1501 acaatagatt atatttttat aaagaagcaa gaatactcaa taaaacttaa agaaattaaa
     1561 caatttctaa aaataccttg aaaatagaac cttatttgcc atttataaat tatataaaaa
     1621 gggattatat aaagtcatat atgaaaaaac tatattatac tatcttttct ttttggaata
     1681 aactatactt taaatataga tatttaattt aaataaattt tacagtatta atagtaaatt
     1741 atcttagaaa ggaatgtgct ttatatgtct acagacaaag ttgttggaat catagatgaa
     1801 gaaacggcag aacttgcagg aattgaatat actggtaaaa tttatgcttc ttctggtgtt
     1861 ataaaacata taaagaaaaa acatagatgt caactgagta aggatatttt taatgatata
     1921 atagacacta taaaagtggt attaaagtca cctgaatata taggaagtca tcctaaaaaa
     1981 cctggaaaaa gtgtggagtt tataaaaaaa attgataatt atattttagt agctacagaa
     2041 ttagatacaa aaaaaggata tttatatgta tcttcaatgt atgctataaa agaagcaaaa
     2101 ttagaaagta gagtaaacaa tggaagagta aaacaatatg atggttaaca taatattatt
     2161 aattttcccc aaaatattga aaaatattat tatataagtt ataatattaa tatacacgta
     2221 gatatttatt agttagattt gaggttggaa aaggcaccca atacgctact atagagtagt
     2281 taatagagat gcaggatacg ccgccctgcc aaatctaatt aaattaaaaa gagaggatta
     2341 attcctctct ttttgttttt ttgagatact aatttaatct atatagaaag attgaaggtc
     2401 atttatacta aaagttttca gttatataca ttaaactaat taaataagat attta
//
LOCUS       DAMJWP010000326         2451 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.333, whole genome shotgun sequence.
ACCESSION   DAMJWP010000326 DAMJWP010000000
VERSION     DAMJWP010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.333"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            24..323
                     /locus_tag="PC700_003519"
     CDS             24..323
                     /locus_tag="PC700_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582782.1"
                     /translation="MKWIKWYSITCICIFIVVAFYMFIFPNKIETIDTSSAYSFVEKK
                     VPNSAVYQGYKKNPVDGTTTIYYSYDNSTHIVRLSHPEDYSRKINWDKVSNIRFD"
     gene            647..2302
                     /gene="ilvD"
                     /locus_tag="PC700_003520"
     CDS             647..2302
                     /gene="ilvD"
                     /locus_tag="PC700_003520"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HDF2582783.1"
                     /translation="MRSDIKKGIEGAPKRALMYGMGLTKEEIERPLIGIVNAQNEVIP
                     GHLHLDEIAEAAKNGVRMSGGLPLEFPAIGVCDGIAMGHVGMNYSLASRELIADSIEA
                     MAMAHGFDALVLIPNCDKIVPGMLMAAARLNIPSIVVSGGPMLPGKKNGKVYDFNSAM
                     EGVGACKDGTVSEEELEELAMNSCPGCGSCSGLFTANSMNCLTEALGMGIPYNGTAAS
                     HSGERKRIAKYAGMYVMELLKNDIKPRDILTIDAFKNAIAVDMAMAGSTNTVLHLPAI
                     AYESGIEFNLDFFDEISEKTPCLTKLSPSGKHHIEDLHMAGGIPAIMNELSKINGINL
                     DCKTVTGKTIGENIRNCEIENEEVIHTLKNPYSNQGGLAILKGNLALNGAVVKKSAVA
                     EEMLVHEGPARVFNSEEEAVNAIFGKKINKGDVIVIRYEGPKGGPGMKEMLSPTSAVA
                     GMGLDKHVALLTDGRFSGATRGASIGHISPEAMEGGLIGLVEEGDIISINIPDKKLEL
                     KVDEVEIENRKLKFKPLEPKIKHGYLSRYAKLVTSANTGAVLK"
BASE COUNT          944 a          271 c          475 g          761 t
ORIGIN      
        1 tttggtaaag taggaggaat tttatgaaat ggattaaatg gtatagtatt acatgcatat
       61 gtatttttat agtagtagct ttttatatgt ttatatttcc taataaaata gaaactattg
      121 atacttcatc tgcatatagc tttgttgaaa agaaagtacc aaatagtgct gtatatcaag
      181 gatacaaaaa gaatccagtt gatggtacta ctacaattta ttacagctat gataattcaa
      241 ctcatatagt tagattatca catccagaag actattcaag gaagattaat tgggataaag
      301 tttcaaacat cagatttgat taatctttaa tatttataaa agtctatata tctaaggcaa
      361 atatattatt aatcttattc atagttgagc actttctatt atagaagtgc ttcttctgat
      421 ttatatcata aaatattact caacaatttg taaaatatat tagtttataa aatatattga
      481 aaagataata ttatataata cgattgtgta agatattgat aaaaaatcgt aataaatttt
      541 cattagtata tgctaatatt tttttacaat tttcgtaatt aattaataga aaaatatatt
      601 ataataatat tatgaaaatt taaaaaagta agaaagaggt aatattatga gaagtgatat
      661 aaaaaaagga atagaaggag ctccaaagag agcgttaatg tatggaatgg gtcttactaa
      721 agaagaaatt gaaagaccat taataggtat agtaaatgca caaaatgagg tgattcctgg
      781 tcatctgcat cttgatgaaa tagcagaagc tgcaaaaaat ggagtaagaa tgagtggtgg
      841 tttgccatta gaatttcctg ctataggggt atgtgatggt atagctatgg ggcatgtcgg
      901 aatgaattat tccctggctt caagagaatt aatagcagat tcaatagaag ctatggctat
      961 ggcacatggt tttgatgctt tggtacttat acctaattgt gacaagatag tgccaggtat
     1021 gttaatggct gctgcaagac ttaatatacc aagtattgtt gtgagtggag ggcctatgct
     1081 tccaggtaaa aaaaatggaa aagtatatga ttttaactct gcaatggagg gtgttggagc
     1141 atgtaaagat ggaacagtaa gtgaagaaga attagaagag ttagccatga actcttgtcc
     1201 tggatgtggt tcatgctctg gacttttcac agcaaatagc atgaattgtt taacagaagc
     1261 tttaggcatg ggtataccat ataatggtac tgctgcgtca cattctggag aaagaaaaag
     1321 aatagcaaaa tatgcaggta tgtatgttat ggagttactt aagaacgaca taaaacctag
     1381 agatatttta acaatagatg cttttaaaaa tgctatagct gttgatatgg caatggctgg
     1441 ttctacaaat acagtacttc acttacctgc aatagcttat gaatcaggaa tagagtttaa
     1501 cttagatttt tttgatgaaa taagtgaaaa aactccttgt ttaacaaaat taagtccaag
     1561 tggaaaacat catattgaag atttacatat ggcaggagga ataccagcta taatgaacga
     1621 actttcaaag ataaatggaa taaatttaga ttgcaaaacc gtaacaggca agactatagg
     1681 ggaaaatata agaaattgtg aaatagaaaa tgaagaagta atacatacat taaagaatcc
     1741 atatagtaac caaggtgggc ttgcaatatt gaaaggaaat cttgctctaa atggagctgt
     1801 tgtaaaaaag tcagcagttg cagaagaaat gttagttcat gaaggaccag caagagtttt
     1861 taattcagaa gaagaagctg taaatgctat ttttggtaaa aaaataaaca aaggtgatgt
     1921 tatagttata agatatgaag gtccaaaggg tggtccagga atgaaagaaa tgctatctcc
     1981 tacatcagca gttgcaggaa tgggacttga taagcatgta gcacttctta ctgatggtcg
     2041 tttttcagga gcaactagag gagcatctat aggccatatt tctccagaag ctatggaagg
     2101 tggtttaatc ggactagttg aagaaggaga tataatttct ataaacatac cagacaaaaa
     2161 attagagtta aaagtagatg aagttgaaat agaaaataga aaattaaaat ttaaaccttt
     2221 agaaccgaaa ataaagcatg gatacttaag tagatatgct aaattggtaa catcagcaaa
     2281 tacaggagca gttttaaaat agtgaactat atttacgaaa attagaaaaa attaagaaaa
     2341 tattaattgg atatcttagg tgttatagca ctaatgaggt atccttttta ttttgtatga
     2401 aaaatatact gtttgtatac aaaaatatta aagatatttt agtaagtttt g
//
LOCUS       DAMJWP010000327         2436 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.334, whole genome shotgun sequence.
ACCESSION   DAMJWP010000327 DAMJWP010000000
VERSION     DAMJWP010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.334"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            308..1834
                     /locus_tag="PC700_003521"
     CDS             308..1834
                     /locus_tag="PC700_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF2582784.1"
                     /translation="MNESYWFLNSSPKEYNKLGENIKTDCLIVGGGITGLTTAYLLAK
                     EGKKVVLVEADKIGYGTSGRNTGKVTCQHDIFYSKIEKKYGLDKAKSYYNANNEALNL
                     VEQIIEENNIKCDFKRETSFVFTEKEDTIKNIKDEYRTCKKIGINCEYHETIENIPLD
                     IKGAISFTNQGQFNPKKYIDGLAKAAVNLGLKIYENTPVVDLEKGKICRVKTREDNII
                     EAENVIISSHSPWYDGLNFYFAKEYAERAYLMVAVLENKLADGMFISIDDPSITFRQY
                     NNGSENLLIFGGGDHKVGQGGTEKEIFDDLEHYGKEVFKVKDFKGKWSAQDNMSFDNV
                     PYIGYINKREDNIYVATGFSKWGITNGTAAGIIIKDLIINNNSDYKDTFNPSRLGSYF
                     SKDFIKENANVAINYVSGKLKIGSGDMPKNNGEGKIVNIDGKRYGVYKDDNGDFYIVD
                     TTCTHLGCELNFNSEEKTWDCPCHGSRFDYKGNILEGPALKPLKLYGHGDNDVNPKLL
                     "
     gene            1862..2413
                     /locus_tag="PC700_003522"
     CDS             1862..2413
                     /locus_tag="PC700_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582785.1"
                     /translation="MNKLLKPITILLYIVYILSAVFFVANKEYGSLGLVAISLVGTFI
                     LSFINKQTERLLSKELYIMLALFIMFSSLLGTCFNFYDINYYDDFLHLWSGIISCIVA
                     FSIIKYFYTQREISKMSKVFLVIFLFMFSMGVASLWEIGEFLMDTFVGTTTQAGGLED
                     TMIDMVDALIGTIITIPYYSKKI"
BASE COUNT          957 a          211 c          438 g          830 t
ORIGIN      
        1 cagaaaaata ttaaacgaac taaatcaata aaaaagttgg tggctaacaa tttataattg
       61 ttagcacaat ataatcttat gtattgaaat ctagaatatc ctcattttta cttagcataa
      121 atactattga ttaagaaaat aatatagaaa ttataaatat atataagttt agtctggtgt
      181 ataaaacaag aaaactaagt gatttttcat ttagcttttt attaagctta ttttttatac
      241 tatttataat aaataaactc catgatggga aaactattac tatttaatat gtaaaggagt
      301 attttaaatg aatgaatcat attggttttt aaactcaagt ccaaaagaat ataacaaatt
      361 aggtgaaaat ataaaaacag attgtcttat agtaggtggt ggaataactg gattgacaac
      421 tgcatattta ttagcaaaag aaggtaaaaa agtagtgctt gttgaggctg ataaaatagg
      481 atatggaact tctggaagga atactggtaa ggtaacttgt cagcatgata tattttactc
      541 taagatagag aaaaaatatg gattagataa agcaaaatca tattataatg caaataatga
      601 agctttaaat ttagttgaac aaataataga agaaaacaat ataaaatgtg attttaaaag
      661 ggaaacttct tttgtattta cagaaaaaga ggatactata aagaatatta aagatgaata
      721 tcgtacatgt aaaaaaatag gaataaattg tgagtatcat gaaactatag aaaatattcc
      781 attagatata aagggggcta ttagttttac taatcaaggt caatttaacc caaagaagta
      841 tattgatgga ttggcaaaag cagcagtaaa tttagggcta aaaatatatg aaaatacacc
      901 agtagtagat cttgaaaaag gaaaaatatg tagggttaaa acaagagaag ataatataat
      961 agaagctgag aatgttataa tttcatctca tagtccatgg tatgatggat tgaattttta
     1021 ttttgcaaaa gaatatgcag agcgtgctta tttaatggtt gcagttttgg aaaataaatt
     1081 agcagatggt atgtttataa gtatagatga cccatcaata acttttagac aatataataa
     1141 tggaagtgaa aatttactga tttttggagg aggagaccat aaagttggcc agggaggaac
     1201 tgagaaagaa atatttgatg atttagagca ttatggtaaa gaagtcttta aggtaaaaga
     1261 ttttaagggg aaatggtctg ctcaagataa tatgagtttt gacaatgttc cttatattgg
     1321 ttatatcaac aaaagagaag acaatattta tgtagctaca ggattctcta aatggggaat
     1381 tactaatgga actgctgctg gaataataat aaaagatttg ataataaata ataattctga
     1441 ttataaagat acttttaatc catccagatt aggtagctac ttttcaaaag attttataaa
     1501 ggaaaatgca aacgtagcaa taaattatgt aagtggaaaa ttaaaaatag gtagtggaga
     1561 tatgcctaaa aataatggag aaggaaagat tgttaatatt gatggaaaaa gatatggtgt
     1621 atataaggat gataatggag atttttatat tgtagacacg acatgtactc atttaggatg
     1681 tgaacttaat tttaacagtg aagaaaaaac atgggactgt ccatgtcatg gttctagatt
     1741 tgactataag ggaaatatac ttgaaggacc agctcttaag ccattaaaac tttatggaca
     1801 tggtgataat gatgttaatc ctaagttatt ataaaattat ttttaggaag gtaaagtgta
     1861 tatgaataaa cttttaaagc caataactat actattgtat atagtttata ttttatctgc
     1921 cgtatttttt gtagcaaaca aagaatatgg aagtctaggt ctagtagcaa ttagtttagt
     1981 tggtactttc attttgagtt ttataaataa gcaaactgaa agattattga gtaaagaatt
     2041 atatataatg cttgctttat ttataatgtt ttcatcattg ttaggtactt gttttaattt
     2101 ttatgatata aattattatg atgatttttt gcatttatgg tcaggtataa tatcttgtat
     2161 tgtggctttt tctataatta aatactttta tacacaaaga gagataagta aaatgagtaa
     2221 agtgttttta gttatatttt tatttatgtt tagtatggga gtagcgagtt tatgggaaat
     2281 aggtgaattt ttaatggata cttttgttgg gactactaca caggccgggg ggttagaaga
     2341 tactatgatt gatatggtag atgctttaat aggcacaatt ataactattc cttattatag
     2401 taaaaaaatc taatatatag ttttaaattt tttata
//
LOCUS       DAMJWP010000328         2408 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.335, whole genome shotgun sequence.
ACCESSION   DAMJWP010000328 DAMJWP010000000
VERSION     DAMJWP010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.335"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..247
                     /locus_tag="PC700_003523"
     CDS             <1..247
                     /locus_tag="PC700_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDF2582786.1"
                     /translation="NVNKNKIKDEDVEKILRDTNYISNYNVDKYDEGRSVILESIVNT
                     KSLGDKIICLNLLQTLNGVKEMYRISNITKVSIKEAA"
     gene            462..1319
                     /locus_tag="PC700_003524"
     CDS             462..1319
                     /locus_tag="PC700_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2582787.1"
                     /translation="MSKDVKVKRNKKYDTFSITMELERPLLYDEVENKDDKNNNRKFN
                     IKNKIFKGIGILFVLILLGFFIWINKTYEPQKLAKEALVSNSKVEVTVNENISFTPKG
                     RKVSKGLILYPGAKVEIESYAPLARKIAESGYEVVIVKMPLNLAILGTNKAQKVMDSY
                     NNIEHWVIGGHSLGGVAASNFARDNKLIEGVVFLASYPMGDELKELGKKVISIWGSKD
                     GVVNFKSLVESKQKLPDDTTYVEIEGGNHAQFGDYGKQKGDNDAIISQEKQLNITANS
                     IIKFLKNIS"
     gene            1416..2285
                     /locus_tag="PC700_003525"
     CDS             1416..2285
                     /locus_tag="PC700_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2582788.1"
                     /translation="MEYDMPLYRPPSEAYSLIIQVTLGCSHNKCTFCSMYKSKKFKIK
                     PLEVIKNEIDIFRRYYKHVERIFLADGDALIIPMEKLRDIILYIKEVFPECERITMYG
                     SPKSIEKKSDDELKELRKLGVKMIYLGLESGSNEVLEDIKKGFSSEQLIKVGRKVKKS
                     GIKLSATVIAGLGGTKKTHQHAVDTGKMLGAISPDYVGVLSLMVEPNTELYDLLKAGE
                     FTILEDKAVLQEIKEMIKNIDANEKIVFRSNHASNYANLKGVLPDDKQRLIDEIDYYL
                     SNQKLKREEYRRL"
BASE COUNT         1026 a          225 c          418 g          739 t
ORIGIN      
        1 aaatgttaat aaaaataaaa taaaagatga agatgttgaa aaaatattga gagatactaa
       61 ttatatttca aattacaatg tagataagta tgatgaagga agaagtgtaa ttttagagag
      121 tattgtaaat actaaaagtc taggtgacaa gataatatgt ctaaacttac tgcaaactct
      181 aaatggtgtc aaagaaatgt atagaataag taatataact aaagtttcaa taaaagaagc
      241 agcttagata ttaaattata gaatagccat atatttaaaa taaagtatat ggctatttgt
      301 tttataaaat actaaaagaa aataatatat aaaaaacaaa aaacatatat ttatgaattt
      361 tatatgaaaa tagttaaaat attgattttt ttcattggtt gaaaaaacaa tttacttagg
      421 ataaactata atagtattca aatattatgg gggttagatt tatgagtaaa gatgtaaagg
      481 taaagcgtaa taagaaatat gatacattta gtataactat ggaacttgag agacctttac
      541 tttatgatga ggttgagaac aaagatgata aaaataataa tagaaaattt aacataaaga
      601 ataagatatt taagggaata ggtatactat ttgtattgat tttgcttggc tttttcatat
      661 ggataaataa aacatatgaa cctcaaaagc tggcaaaaga agctctagtc agcaatagta
      721 aagttgaagt aactgtaaat gaaaacattt catttacacc aaaaggaaga aaagtttcta
      781 aaggtttaat tttgtatcca ggagctaaag tagaaataga atcttatgct ccacttgcaa
      841 gaaaaatagc agaaagtgga tatgaggttg taatcgtaaa gatgccactt aatctagcta
      901 tacttggaac taataaggca cagaaagtta tggattctta caacaacata gaacattggg
      961 taataggagg tcattcatta ggaggagttg cagcatcaaa ttttgcacgt gataataaat
     1021 taattgaagg cgttgtattt ttagcttctt atccaatggg tgatgagttg aaagaattag
     1081 gaaaaaaagt tatatctata tgggggagca aagatggagt agtcaatttt aaaagtcttg
     1141 tagaatcaaa acaaaaactt cctgatgata caacttatgt tgagattgaa ggaggaaatc
     1201 atgcacaatt tggagactat ggaaaacaaa agggagataa tgatgctatt ataagtcaag
     1261 aaaaacaact taatattact gccaatagta tcattaaatt tttaaagaat atatcttaaa
     1321 ctaaaattta aaaaatataa ttttataagt tattatttaa agtaaatatt aataatataa
     1381 tagaatgaat attttattac aagggggatt ttaatatgga atacgatatg ccactttaca
     1441 gaccaccaag tgaggcttat agtcttataa tacaagtcac actaggatgt tctcataaca
     1501 aatgtacgtt ttgtagtatg tacaaatcta aaaaatttaa aataaagcca ctagaagtaa
     1561 taaaaaatga aattgatata tttagaagat attataagca tgtagaaaga atattcttag
     1621 cagatggaga tgcattgata ataccaatgg agaaattaag agatattatt ttatatataa
     1681 aagaagtttt tccagagtgt gaaagaatta ctatgtacgg ctctcctaaa tctatagaga
     1741 aaaaatctga tgatgaactt aaagaattaa gaaaacttgg tgttaaaatg atttatctag
     1801 ggcttgaaag tggaagcaat gaagtattag aagatattaa gaaaggtttt agtagtgaac
     1861 aattaataaa agttggtaga aaagttaaaa aaagtgggat taaattatca gccactgtaa
     1921 tagcaggtct tggaggaact aaaaaaacac atcagcatgc agttgataca ggaaaaatgt
     1981 taggagcaat ttcaccagat tatgtgggtg tacttagttt aatggtagaa cctaatactg
     2041 aactttacga tttacttaag gcaggagagt ttacaatact agaggataag gctgtgttac
     2101 aagaaattaa agagatgata aagaatatag atgctaatga aaaaattgta tttagaagta
     2161 atcatgcatc aaattatgct aatttaaaag gtgtattacc tgatgataaa caacgtctaa
     2221 ttgatgaaat tgattactat ctaagtaacc aaaagctaaa aagagaagaa tatagaagac
     2281 tatagttttt gatatatcaa tctatattga taggtaaata ctaagattat ttagatacaa
     2341 aaaatcatgc tacattaata gttgcttact actgatgtag catgattttt ttattaaaaa
     2401 acaaaatg
//
LOCUS       DAMJWP010000329         2402 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.336, whole genome shotgun sequence.
ACCESSION   DAMJWP010000329 DAMJWP010000000
VERSION     DAMJWP010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.336"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(152..2245)
                     /locus_tag="PC700_003526"
     CDS             complement(152..2245)
                     /locus_tag="PC700_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2582789.1"
                     /translation="MNKRNFEVILNQLQINIYVTNIHTNEIIFMNKKMKEEYNILDPE
                     GKVCWQVLYPEQNSTCSFCKVLELLKNDKKGVLIKWYEKCNKLNRVFENYDSLITWQD
                     GTVVHMHQSIDIANSTILNKPIKINEFNEISNTKEGKGVFNFSRDNFDYNSTLLYDAL
                     IRGTDEYIYICNMKTGVFRYSPSQVELFDLPGEIVENPLIYWKKIVHPEDWNRFYKSN
                     TEIGKNQMDYHTVEFRAKNRSGEYIWLRCRGQLMRDEFGEPSIFAGIMTQLGKQNKID
                     SLTQLLNYHEFMSVFEDKISNPMIEKLCIVLLNIDDFKNVNEMYDRDFGDNIIKTLAQ
                     SIQSILPDNAELYKLDGDEMGILVDNVEENEILTLYNQIQNMIIHLQLWRKYGLNITI
                     SAGCVIYPKHGDTVKELYKCASYSLQYAKEHGKNRLVFFSQEILKNKMYSLEMMRDLK
                     ASINDDFRGFSLRFQPQVDTQSHKIIGVEVLLRWTNDKCKAISPLEFIPILEENDMIN
                     IVGAWVLRMALRTFRKWIDYYPFFKVSVNVSAVQILEDTFIEDIVKIIDDENFPYQNL
                     VLELTESHTVQNMSILQFKFKALQDLGIYIAMDDFGTGYSSLEVLKFSPIDIVKIDRV
                     FVKDILKSKFDATFIHFIVAICHDVGIKVCLEGVETQEEYDLVKQIKPDYIQGYLFGK
                     PQTATEIFDLLKLDN"
BASE COUNT          813 a          397 c          238 g          954 t
ORIGIN      
        1 aaaatacttt aatcatatac tactcctaat ttaacatccc ttgaaatgct attttcttat
       61 attgcaattt ataattgata tatcttcaat ataataattt aatgtatttt tgattttaaa
      121 gcttttcagt gatattattg ataaattaga attaattatc tagctttaaa aggtcaaaga
      181 tttctgttgc tgtctgcggt tttccaaata aatatccttg tatgtaatct ggctttattt
      241 gtttaactaa atcatactct tcttgtgttt ctacaccttc taaacatact tttattccta
      301 catcatgaca aattgcaaca ataaaatgta taaatgtagc atcaaactta ctctttaata
      361 tatctttaac aaatactcta tcaattttca ctatatctat tggagaaaat tttaaaactt
      421 ctagagacga gtatccagta ccaaaatcat ccatagcaat ataaattcct aaatcttgta
      481 aagccttaaa tttaaactgt aaaatactca tattttgtac tgtatgactt tctgtcaatt
      541 ctaaaacaag attttgataa ggaaaatttt catcatctat aatttttact atatcttcaa
      601 taaatgtgtc ttctaatatt tgaacagctg aaacgtttac actcacttta aaaaaaggat
      661 agtaatcaat ccatttccta aaagtacgta atgccattcg aagcacccaa gcccctacaa
      721 tatttatcat atcattttct tctaatattg gaataaattc caaaggagaa attgctttgc
      781 atttgtcatt agtccacctt aaaagaactt ctacacctat tattttatga ctttgtgtat
      841 ctacttgtgg ctggaatcga agactaaatc ccctaaaatc atcattaatt gatgccttta
      901 aatcccgcat catttctaat gaatacattt tattttttaa tatttcttgg gagaaaaaaa
      961 caagcctgtt tttaccatgc tctttcgcat attgtaaaga gtaactagcg catttataaa
     1021 gttcttttac tgtatctcca tgttttggat atattacaca accagcagaa attgttatat
     1081 ttaatccata ttttctccat agttgaaggt gtataatcat attctgtatt tgattatata
     1141 gagttagaat ttcattttct tcaacattat caacaagaat acccatttcg tccccatcaa
     1201 gtttataaag ttcagcatta tctggtaaaa tagattgtat agactgtgct agtgttttta
     1261 ttatattatc tccaaaatcc ctatcataca tctcattaac atttttaaaa tcgtcaatat
     1321 ttaataatac tatgcataat ttttcaatca taggatttga aattttatct tcaaatacac
     1381 tcataaattc atgataattc agtaactgtg tcaatgaatc aatcttattt tgtttaccta
     1441 attgcgtcat tattcctgca aaaatacttg gttctccaaa ttcatcacgc attagttgtc
     1501 ctctacatct taaccatata tattctccac tacgattttt agccctaaat tccactgtat
     1561 gataatccat ctggttttta cctatttcag tgtttgactt ataaaaacga ttccagtctt
     1621 ctggatgtac aatttttttc cagtaaataa gcggattttc aacaatttct cctggtaagt
     1681 caaataattc aacttgtgat ggtgaatatc gaaatacgcc tgttttcata ttacaaatgt
     1741 atatatattc atctgtacct ctaatcaaag catcataaag caatgtagaa ttataatcaa
     1801 aattgtctct tgaaaaatta aaaactcctt ttccttcttt agtatttgat atttcattaa
     1861 attcattaat cttaattggt ttatttaaga ttgttgagtt tgcaatatct attgattgat
     1921 gcatatgaac aactgtgcca tcttgccatg taattaaact atcataattt tcaaaaacac
     1981 gatttaactt attacatttt tcataccact ttattaatac tcctttttta tcattcttta
     2041 atagttctaa aactttacaa aaactacaag tagaattttg ctcaggatat aatacttgcc
     2101 agcaaacttt tccttctgga tctaaaatat tatattcttc tttcattttc ttgttcataa
     2161 aaataatttc attagtatga atatttgtaa catatatatt aatctgtagt tgatttaata
     2221 taacttcaaa attacgtttg ttcatatact tatctcctcc atagttactg ctctttataa
     2281 caacactgtt aaaattattc tttttagaag atttacttat aacttataca tacacactaa
     2341 ataccttaat ttaattagaa agtttattat ttcatatttt aaattataat tgataaaaaa
     2401 aa
//
LOCUS       DAMJWP010000330         2379 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.337, whole genome shotgun sequence.
ACCESSION   DAMJWP010000330 DAMJWP010000000
VERSION     DAMJWP010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.337"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            46..963
                     /locus_tag="PC700_003527"
     CDS             46..963
                     /locus_tag="PC700_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582790.1"
                     /translation="MENNKCREDFRFTQEYEEDYPNTNERYYENYQVADRYYNYPNKY
                     KEPKIKQCCCKKSMREALELLRYDALRPFVNFNQFAFISDFFIVGANLVGIDLSAPPK
                     DNLSGLDGTFERFSACNCDLIDIAGRVSYPIPVPLTLEGLINTIGTIPGVAELIALID
                     AVIPPTIDLGAILDAILAAIIDFILAASTPLANVDLASLCNLKAVAFDITPADYEDFI
                     ASLGYYLDKKHYKECNCNCDCDDCCCNKGILDNLYMSNINNQVTVVAGSLVLTGVEVL
                     GKKNDVIVLGNSNDSRIYFVCVDSIDYIA"
     gene            complement(1105..2286)
                     /locus_tag="PC700_003528"
     CDS             complement(1105..2286)
                     /locus_tag="PC700_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF2582791.1"
                     /translation="MNKKYIKAGNLIDGTGNSIMQNKGIIIDGDTIVEIEDIRDDLNG
                     YDVYDYLDKTVMPGIMNCHVHLTMEPVGNPKIYYDNVSDVELVVNTIKQVDAYLDSGV
                     TYIRSLGCPKYVDVQLKNLIDKGSIDGPGIVTSGPVICMTGGHGYYFGIESDGVDECR
                     KSARTVLKNGVDCVKIMATGGVTTDGVEPGSPQLTLEEMKAAVDEAIKAGKITATHAQ
                     GRTGIKNAVLAGITSIEHGVYLDDEIIDLMLERGTYLVPTVAAPYFILNAGKESGITE
                     ATLRKCEIVAIPHKESFLKAYKKGIKIAVGSDAGTSYNEHGKTYYEMKLMSDYGMDNM
                     DIIVAATKTASELLRIDKNYGTLEKGKKADVIVVNGNPVENIDVLADVLAVFKLGKKV
                     R"
BASE COUNT          768 a          403 c          304 g          904 t
ORIGIN      
        1 taattatcta tacatttaaa ttaaaatata aaaggagtgt atttagtgga aaataataaa
       61 tgtagagagg actttagatt tacacaagaa tatgaggaag attatccaaa tacaaatgaa
      121 agatactatg aaaattatca agtagctgat agatactata attatccaaa taaatataaa
      181 gaacctaaaa taaaacaatg ttgttgtaaa aaaagtatga gagaggcctt agaacttcta
      241 agatatgatg ctctaagacc ttttgtaaac tttaatcaat ttgcttttat ctcagatttc
      301 tttatagtag gtgctaattt ggtaggtata gatctttcag ctcctccaaa agataattta
      361 tctggacttg atggtacttt tgaaagattt tctgcttgta actgtgattt aatagatata
      421 gctggtagag tatcttatcc tattccagtc cctttaactc ttgagggatt aattaatact
      481 ataggaacta taccaggagt agctgaatta attgcactta ttgatgcagt tattcctcct
      541 acgatagacc ttggggctat attagatgca atacttgctg ctataattga ttttatactt
      601 gctgcatcta ctccattagc aaacgtagat ttagcatcat tgtgtaatct taaagctgtt
      661 gcatttgata ttacacctgc agattatgaa gatttcatag catctttagg ttactatctt
      721 gataaaaaac attacaaaga atgtaattgt aactgcgatt gtgatgattg ctgttgtaat
      781 aaaggtatcc tagataatct ttatatgtca aatataaata atcaagttac tgtagtagct
      841 ggtagtttgg ttctaactgg tgttgaagtt ctaggtaaga aaaatgatgt tatagtactt
      901 ggaaattcta atgattcaag aatatacttt gtatgtgtag attctataga ttatattgca
      961 taagaatgat tttaaagagt tatatctaaa tataaaatag aatctatttt ctaaagtata
     1021 aaagatttaa ttattactaa taaaaaagag tgtctataat tatttaaaaa tttctttaga
     1081 cactcttttt tatttttata ctatttatct aactttttta ccaagtttaa atactgctaa
     1141 cacatctgca agtacgtcta tattctctac tggatttcca tttacaacta taacatctgc
     1201 tttctttcct ttttctagag ttccataatt tttatcaatt cttaaaagtt cagatgctgt
     1261 ttttgtagca gcaactatta tatccatatt atccatacca taatctgaca ttagcttcat
     1321 ttcataatat gtttttccat gttcattata agaagttcct gcatcagaac caactgcaat
     1381 tttaattcct tttttatatg cttttaggaa tgattcttta tgtggaatgg caacaatctc
     1441 acactttcta agagtagctt cagttattcc actttctttt cctgcattta gaataaaata
     1501 tggtgcagcc acagttggaa ctaagtaagt tcctctttca agcatcaaat caattatttc
     1561 atcatctaaa taaacaccat gttctataga agtaattcct gcaagaactg cattttttat
     1621 accagttctt ccttgtgcat gtgtggcagt tattttacct gcttttatag cttcatctac
     1681 agcagccttc atttcctcca aagttaattg tggtgaccct ggctcaactc catcagttgt
     1741 tacacctcca gttgccatta tctttacaca atctactcca ttttttaaaa ctgttctggc
     1801 tgattttcta cattcatcca ctccatcact ttcaatacca aaataatatc catgccctcc
     1861 tgtcatacat ataactggac ctgaagtaac tataccagga ccatctatag accctttgtc
     1921 tataagattt ttaagttgta catccacata tttgggacaa cctaagcttc taatatatgt
     1981 tactcctgaa tccaaataag catcaacttg ttttatcgta tttactacaa gctctacatc
     2041 tgatacatta tcataatata tctttggatt tccaactggc tccattgtca agtgaacatg
     2101 acaattcata atgcctggca taacagtttt atctaagtaa tcgtacacat catatccatt
     2161 taaatcatct cttatatctt caatttcaac tatagtatct ccatcaataa ttataccctt
     2221 attttgcata atactatttc ctgttccatc aattaaattc cctgctttaa tatatttttt
     2281 gttcatctta aatttctccc ccttttaagt acaatgtatt atttatttat tatttctaat
     2341 attctaataa ttaattttac cactataagc attctattt
//
LOCUS       DAMJWP010000331         2366 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.338, whole genome shotgun sequence.
ACCESSION   DAMJWP010000331 DAMJWP010000000
VERSION     DAMJWP010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.338"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(71..973)
                     /locus_tag="PC700_003529"
     CDS             complement(71..973)
                     /locus_tag="PC700_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2582792.1"
                     /translation="MYNKIKYIPINCEIACNKLKRSMPYKWDLNIYRGCEHGCKYCYA
                     IYSHKYINSNNYFEEIYVKTNIVEKLERQLKSSKWKKEVINIGGVTDSYQPIEADYKI
                     MPEILNLLIKYKTPAIISTKSDLILRDYDLIDKLSRITYINVASTITTVNEDTQRLIE
                     PNGVDSMRRFKMLKEFRKTNASVGLHIMPIIPYITDDFDNINSLFRHAKECNVHYVLP
                     GTLYLRGITRGVFFDFVKKEFPELFDKLSILYSAGSLNREYKNQLYKMVNELRYKYSL
                     SSSYSKIMKEKLKNSGDGQISFFD"
     gene            complement(1023..1484)
                     /locus_tag="PC700_003530"
     CDS             complement(1023..1484)
                     /locus_tag="PC700_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HDF2582793.1"
                     /translation="MRLWHKDLIDVLPKNQLVSQWRELLAIKGSIDKKGTPNHLLVNK
                     VLNYSIDEFKFYTKIVHDEMLKRNYKPNELKYTSILKWKNRNFANDISNEHSLNLENL
                     YDDWHNKMYLKQCLYNLEEKATCGGIPINEWNILLCKYSKDYELWSGNIMF"
     gene            complement(1536..1991)
                     /gene="def"
                     /locus_tag="PC700_003531"
     CDS             complement(1536..1991)
                     /gene="def"
                     /locus_tag="PC700_003531"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDF2582794.1"
                     /translation="MAIRKIRTFDDEILRKKSKYVENVDNKIREILNDMAETMYNTPN
                     GGGLAACQVGVLKRLVVIDLGEGLIKLVNPEIIKQEGEQIVVEGCLSFPEVWGKLKRP
                     KKVTVQALNEYGEKIEIKGSGFMAKCLCHEIDHLNGIVFTDKIIEHVKL"
     gene            complement(1963..2226)
                     /locus_tag="PC700_003532"
     CDS             complement(1963..2226)
                     /locus_tag="PC700_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2582795.1"
                     /translation="MKNKIKILREKLGLTQEQLGRLVGTSRQAINAIETGKNEPSIWL
                     AYDISRIFNEPIESVFLFEESERKSRAQISRGEYYGYKKDKNI"
BASE COUNT          761 a          398 c          204 g         1003 t
ORIGIN      
        1 atatttgtca ataaatttta attttaataa attttaatca tcatagtaaa aaaaaatttt
       61 ttatataaaa ctaatcaaaa aaagatatct gtccatctcc tgagtttttt aacttttcct
      121 tcattatttt agagtaactg cttgatagag aatatttata tctaagttca ttaaccattt
      181 tatacaattg atttttatac tccctattaa gtgaacctgc actatatagt attgataatt
      241 tatcaaataa ttcaggaaac tcttttttaa caaagtcaaa aaaaacacct ctagttatac
      301 cccttaagta taaagtccca ggtaaaacat aatgtacatt acattctttt gcatgtctaa
      361 atagcgaatt tatattatca aaatcatctg taatatatgg tataattggc ataatatgta
      421 gaccaactga tgcgttagtt tttctaaatt cttttaacat tttaaatcgt ctcattgaat
      481 ccacaccatt tggctcaata agcctttgtg tatcttcatt tactgtggta attgtagatg
      541 ctacattgat atatgtaatc ctagatagct tatcaattaa atcgtaatct cttaaaatta
      601 aatctgactt tgtagaaata atagctggtg tcttgtattt tatcagcagg tttaatattt
      661 ctggcataat tttatagtca gcttctattg gttgataact atctgtaact cctcctatat
      721 tgattacttc tttcttccat ttactgcttt taagttgtct ctcaagtttt tctactatat
      781 ttgtttttac ataaatctct tcaaaataat tattggagtt aatgtactta tgagagtaaa
      841 tagcataaca atatttacat ccatgctcac atccacgata aatatttaaa tcccatttat
      901 atggcatact cctttttaat ttattacaag ctatttcaca atttattgga atatatttta
      961 ttttattata caaataatca cctctttata tacaataata ctataatgtt aatctatagt
     1021 aattaaaaca tgatatttcc agaccacaat tcataatctt tactatattt acaaagtaat
     1081 atattccatt catttattgg tattcctcca catgttgctt tttcttctaa attatataag
     1141 cattgcttta aatacatttt attatgccaa tcatcatata aattttctaa atttaaacta
     1201 tgttcattag atatatcatt tgcaaaattt ctatttttcc attttaatat acttgtatat
     1261 ttaagttcat taggtttata atttcttttt aacatttcat catgtacaat ttttgtataa
     1321 aatttaaatt catctatact ataattcaat actttattca ctaatagatg gtttggagta
     1381 ccttttttat ctatagaacc ttttatagca agtagctctc tccattgact tactaattga
     1441 tttttaggta atacatcaat taaatcctta tgccataatc tcataattta actccttact
     1501 ataaaatatt ttataaattc aataaccaat ctatcttaca attttacatg ttcaataatt
     1561 ttatctgtaa aaactatacc atttaaatgg tctatctcat gacaaagaca ttttgccata
     1621 aaacctgaac ctttaatttc tattttctca ccgtattcgt ttagagcttg cactgtaact
     1681 ttttttggtc tttttaattt accccatact tctggaaaac ttaaacatcc ttcaacaaca
     1741 atttgttccc cttcttgttt aataatttca ggattaacaa gcttaataag tccttctcct
     1801 aaatctataa caactaatcg ctttaatact cctacttggc atgcagctaa tcctcctcca
     1861 tttggagtat tgtacatggt ctctgccatg tcatttaata tttctcttat cttattatct
     1921 acgttttcta catacttact cttttttctt aaaatttcat catcaaatgt tcttatcttt
     1981 cttatagcca taatattctc ccctacttat ctgtgctctt gattttcttt cactttcttc
     2041 aaataaaaat acactttcaa ttggttcatt aaaaattctt gaaatatcat atgccaacca
     2101 tattgaaggt tcatttttac cagtttctat agcatttata gcctgtctag atgttccaac
     2161 taatcttcct agttgttctt gtgtcaatcc taatttttct cttaatatct ttattttatt
     2221 tttcatattt gctccattca tgtaaggtta actttacatc ttaaatgtaa catttttcaa
     2281 cattactgtc aagtcaacct tacatttata tattaaattt tcttatttaa ctattttttt
     2341 actaaacttt ttaaataata ataaaa
//
LOCUS       DAMJWP010000332         2360 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.339, whole genome shotgun sequence.
ACCESSION   DAMJWP010000332 DAMJWP010000000
VERSION     DAMJWP010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.339"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..344
                     /locus_tag="PC700_003533"
     CDS             <1..344
                     /locus_tag="PC700_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2582796.1"
                     /translation="IKENDIICIEDLQVKNMIRNRKLSRLISDVSWSEFVRQLEYKAN
                     WHGRQIVKVGKFFASSQICNKCGYKNEEVKDLNIREWICPSCNEIHDRDINASINILK
                     EGLRLITIQNK"
     gene            628..1074
                     /locus_tag="PC700_003534"
     CDS             628..1074
                     /locus_tag="PC700_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDF2582797.1"
                     /translation="MKLIWLIVAVLFGIAEMLTPSLTLIWFSVGAVILIFLSSFIESI
                     FLQILIFAVISIAMLVVATKKIVKKDKGYKSNTNLQAMMSKKGIVTEEISPNNTGLVV
                     VEHETWTAISIDGEKIEKGSTVEVLKIEGVKLVVKKVDATVNVTNQ"
     gene            1095..2138
                     /locus_tag="PC700_003535"
     CDS             1095..2138
                     /locus_tag="PC700_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HDF2582798.1"
                     /translation="MGTKIVLSIVLIVVVVAISLTCIRVIKQSKVGIIMRLGKFQKVA
                     ETGVHFLIPFLDKMAYVIDLREIVIDFPPQPVITKDNVTMQIDTVVYYKVTDPVRYVF
                     EIANPIAAIENLTATTLRNIIGELDLDETLTSRDIINVKMRTILDEATDKWGIKVNRV
                     ELKNIMPPQDIQVAMEKQMRAERERREAILQAEGNKSAAILQAEGEKQSAILTAEAKK
                     EAMVRVAEGEKESAILVAEGEAEAIRQTAIAKAQGEAEMIKRTQMATAEGLKLVFSAM
                     KEADIDNNILALKSMEALEKMAEGKSTKLVLPSEAVNFLGTFKGIKEVMSDDNKEVLD
                     IKEVLNDNESLKK"
BASE COUNT          967 a          245 c          444 g          704 t
ORIGIN      
        1 taataaaaga gaacgatatt atttgcatag aagatttaca agtaaagaat atgattagaa
       61 atcgtaaact atctcgctta atttctgatg tgtcatggtc agaatttgtt cgtcaattag
      121 aatacaaggc taattggcat ggtagacaaa ttgtaaaggt aggtaaattc tttgcaagtt
      181 cacaaatatg caataaatgt gggtataaaa acgaggaagt gaaggactta aatataagag
      241 aatggatttg tcctagttgt aatgaaattc acgatagaga tataaatgca agtataaata
      301 tattaaaaga aggattaaga ctaataacga ttcaaaataa ataacttatg taagaaccgt
      361 aggaactacg gggatagcct gtggagaatt agtaagacat atgttagtat gcaaaattct
      421 atgaagcagg aaccctgtga ctttagtcat gggaggttca gactactttg taacaatttt
      481 aacaaagtat gttgatataa tactattaaa ttaatcttag attagtttat tctattgatt
      541 tgtagtttaa atttatattc aaaaacattg tataaacata taatttatat agggttttat
      601 aattcatatg aaaagagggt agatagtatg aagttaatat ggttgatagt agcagtctta
      661 tttggaatag cagaaatgtt aacaccaagc ttaacattga tatggtttag tgtaggagct
      721 gtaatactta tatttttaag tagctttata gagagtattt tcttacaaat tttaatattt
      781 gcagtaattt ctatagcaat gttagtcgta gctactaaaa aaatagtaaa aaaagataaa
      841 ggctataaat ctaacactaa tttacaagca atgatgtcta aaaaaggtat tgttacagaa
      901 gagatttctc caaataacac aggtctagtt gtagttgaac atgaaacatg gacggctatt
      961 tctatagatg gtgaaaagat agaaaaaggt tctacagtag aagtattaaa aattgaaggg
     1021 gtaaaattag ttgttaaaaa agtagacgcc acagttaatg tgacaaacca atagaataaa
     1081 ggaggattta tagtatgggt actaaaattg ttttaagtat tgtattaata gtagtagtcg
     1141 tagcaataag tttaacgtgt ataagagtta taaaacaatc taaagtaggt ataataatga
     1201 gacttggtaa gtttcaaaaa gtggctgaaa caggtgtaca tttcttaata ccatttttag
     1261 ataaaatggc atatgtaatt gaccttagag aaattgtaat agattttcca cctcaaccag
     1321 ttataactaa agataatgta actatgcaga ttgatacagt tgtatattat aaagttacag
     1381 atccagttag atatgtgttt gagatagcta atccaatagc tgctattgaa aatttaacag
     1441 ctacaacact aagaaatata attggtgaac ttgatttaga tgaaacatta acatcaagag
     1501 atataataaa tgtaaaaatg agaactatcc ttgatgaagc aacagataaa tggggtataa
     1561 aagtaaatag agtagagtta aaaaatataa tgcctcctca ggatattcaa gttgcgatgg
     1621 aaaaacaaat gagagcagaa agagaaagaa gagaggcaat acttcaagca gaaggtaata
     1681 agtcagctgc aatacttcaa gcagaaggtg aaaaacaatc tgcgatatta acagcagaag
     1741 ccaaaaaaga agctatggta cgtgtagcag aaggtgaaaa agaatctgct atattagtgg
     1801 cagaaggtga agctgaagct ataagacaga ccgctattgc taaggcacaa ggtgaggctg
     1861 aaatgataaa aagaactcaa atggcaacag cagaaggttt aaaattagtt ttttcagcaa
     1921 tgaaagaggc tgatatagac aataatattc tagcattaaa atctatggaa gctcttgaaa
     1981 aaatggctga aggtaagtca acaaaactgg ttttaccttc agaagcagtt aatttcctag
     2041 gaacattcaa aggaataaag gaagttatga gtgatgataa caaagaagta cttgatataa
     2101 aagaagtttt aaatgataat gaatcattaa aaaaataaaa gagttttgtt atcataggaa
     2161 ggatatggaa attaaaggtc gaattaatta aatatagcac aaaaatgcac gttgaaaaga
     2221 agaggactta taaattttta agtcttcttc tttgttttta ttagaaagat gaaattataa
     2281 aaatgaatta acagaatctt tatacaattt atcctttact aatgtaaaaa gtgttatata
     2341 atattatata aaacatatag
//
LOCUS       DAMJWP010000333         2351 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.340, whole genome shotgun sequence.
ACCESSION   DAMJWP010000333 DAMJWP010000000
VERSION     DAMJWP010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.340"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            335..2230
                     /locus_tag="PC700_003536"
     CDS             335..2230
                     /locus_tag="PC700_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp8"
                     /protein_id="HDF2582799.1"
                     /translation="MLSNKKRSMAIVMAGATVMSAAAPIFADNTVTENVDKNYTVSAK
                     DSAKLIEEVRKALEVKFEDTKAGANVNDRVYDIKVDNVNLTNATQLQNKINSLTEGQS
                     LKVTIQDKGHQVLGGKVVDYKIENYKTAQEIVDAVNAYNATLAEDSDNKLTATIKSTN
                     TVEVKRAKDSANVITLNVGDQHLDFSKVITSEEGTFEGYEKSYSDIDSKELHTITVKN
                     ADLQDISAEELFDGIRLTTLGREIVNKVKNGYALTFENEAILTQEQEDSDDKDKPEKS
                     SFDIVLSKANEKSETISVSSKNHKLVRDLHKVLTDVKDGKELKVEVLSGDSRFTTAVE
                     VSKERFKDGEAEAIILVGEDAIVDGLASAPLASQKNAPILLSKKDSLPSEIEAEILRV
                     LGSNLSSKKIYIVGGESKVSKETEEKLSKLGVSKVERVSGEDRFETSLEIAKQLKDTF
                     KTAFVVGGNGEADAMSISARAAQFGAPIIVTGNELDANAEKLLKGKELEIVGGENSVS
                     KEVEDKLVDIDLNNKVERLAGENRKDTNAKVINKYYAGATKAYVAKDGYVGGNGQLVD
                     ALTAAPLAASSKAPIVLTTEELSKSQEEVVELRLKNATKLVQIGEGIAKNAIEKIAEK
                     INLFTKN"
BASE COUNT         1033 a          237 c          438 g          643 t
ORIGIN      
        1 tatggaggta gatattaatt tagtgatata tatttaagta ataagagcaa agcaaaaatt
       61 agtgcatagt agtatataaa aatggagttt gaatactttt gttagtatat gattgtttag
      121 tagaaaatca ctataaaata gggatttcta tctttgagca taattataga gagctaagta
      181 gggggaaggg gttcaagaag atattattag tgtttgtgtt agtaaattat gtaaacacat
      241 aaaatatatt gaaaaataat attatatata atattattta tgtgtgggaa atacgaatat
      301 aaaaaataaa aaataatatt cggaggacat aaatatgtta agcaacaaaa agagaagtat
      361 ggcaatagta atggcaggtg ctacagttat gagtgcagca gcaccaatat tcgcagacaa
      421 tactgtgaca gaaaatgttg ataaaaatta cacagtaagt gcgaaagatt ctgctaaact
      481 aatcgaagag gttagaaaag cattagaagt taagtttgaa gatacaaaag caggagcaaa
      541 tgtaaatgat agagtttacg atataaaagt agacaatgta aacttaacta atgcaactca
      601 attacaaaat aaaataaact ctttaacaga agggcaaagt ttaaaagtta ctattcaaga
      661 taaaggacat caagtgttag gtggaaaagt agttgactat aaaattgaaa actacaaaac
      721 tgctcaagaa atagtagatg cagttaatgc ttacaatgca actttagcag aagattctga
      781 taacaagtta acagcaacta taaaatctac aaatacagtt gaagtaaaaa gagcaaaaga
      841 ttcagcaaat gttattacat taaatgtagg agaccaacat ttagatttct caaaagtgat
      901 aacatcagaa gaaggtactt ttgaaggtta tgaaaagagt tatagtgaca tagattcaaa
      961 agaattacat acgataacag ttaaaaacgc agatttacaa gacatatcag cagaagaatt
     1021 atttgatgga attagattaa ctacattagg aagagaaata gttaataaag ttaaaaatgg
     1081 atatgcttta acatttgaaa atgaagcaat ccttactcaa gaacaagaag atagtgatga
     1141 taaagataaa ccagaaaaat caagctttga tatagtttta agcaaggcaa atgaaaagtc
     1201 agaaacaata agtgtttcaa gtaaaaacca taagttagtt agagacttac acaaagtatt
     1261 aactgatgta aaagatggaa aagaattaaa agtagaggtt ttatctggtg attcaagatt
     1321 tacaacagca gtagaagtta gtaaagagag attcaaagat ggagaagcag aagctataat
     1381 tttagttgga gaagatgcta tagttgatgg attagcatca gcaccacttg cctctcaaaa
     1441 aaatgcacca atattattat ctaaaaaaga ttcactacca tcagaaatag aagctgaaat
     1501 attaagagta cttggaagta atctatcttc taagaaaata tatatagtag gtggagaatc
     1561 taaagtatca aaagaaactg aagaaaaact ttctaaatta ggtgtaagta aagttgagag
     1621 agtttctgga gaagatagat ttgaaacttc tttagaaata gcaaaacaat taaaagatac
     1681 atttaagact gcttttgtag taggtggaaa tggagaagct gatgctatga gtatatcagc
     1741 tagagctgct caatttggtg ctccaataat agttacaggt aacgaattag atgcaaatgc
     1801 tgaaaaatta ttaaaaggaa aagaattaga aatagtgggt ggagaaaatt ctgtatcaaa
     1861 agaagttgaa gacaaattag ttgatataga tttaaataat aaagttgaaa gattagctgg
     1921 agaaaataga aaagatacta atgctaaagt aatcaataaa tactatgcag gtgcaactaa
     1981 agcatatgta gcaaaagatg gttatgtagg tggaaatgga caattagttg atgcacttac
     2041 agcagcacca cttgcagcta gttcaaaggc tccaatagta ttaactacag aagaactttc
     2101 taaatcacaa gaagaagtag ttgagttaag acttaagaat gctactaagt tagtacaaat
     2161 aggtgaagga atagctaaaa acgctattga aaaaatagca gaaaaaataa acttatttac
     2221 taagaactaa taagatatag gaattaatat taaaagttta tttagataat atatgattat
     2281 acaatgcgtt aatatgtaag acatagtgta gatattataa aatatacaaa taacaagata
     2341 aaataataaa a
//
LOCUS       DAMJWP010000334         2343 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.341, whole genome shotgun sequence.
ACCESSION   DAMJWP010000334 DAMJWP010000000
VERSION     DAMJWP010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.341"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            940..2295
                     /locus_tag="PC700_003537"
     CDS             940..2295
                     /locus_tag="PC700_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDF2582800.1"
                     /translation="MSSTVKKKGKFPMGFYICSTTFSFERAAYYASKYLIYIFLTTAI
                     VHGGLGIDKGQAAIMQANLVAFTYLAPIIGGYISDRWIGARYTIPIGMLIMGVGYYLG
                     SIATTVSMVNAMIILVSIGTAFFKGNVSAVNGQLFDSQEELDTAFSVQYSFVNIGSFI
                     GTIAVGILYLKTFAKNGVLGFSQCFFIAAVLCVIGAIWFIYGWRFLGNAGKRPFKEGV
                     VAEKVEEKDKSPLTSMDKKRIWAIILISFFSVIFWVFWYLTYLAVYDYGAAFVNMNVG
                     GFDVPLAWFDSLNSLVCIVLGPVLGALWFKLASRPQGDMSLFKKTGLGLIFLGLAFLM
                     LVGAEFSRGVGAPETAKASILWIIMFGILLSLGEMLFSPLGNSFVSKYAPKKLLGVLM
                     GVWTFATFIAGKGYGYIYAFTLKFDMIKVYTIIPVILFVAAILLFLSDKTLSRLVEDD
                     K"
BASE COUNT          828 a          256 c          396 g          863 t
ORIGIN      
        1 tactgcatac aaaaatatat ttgagcaaat aagtaaagta ataaaaaaat caagatttca
       61 tatctaaaaa ctattctgaa cattaattta atttatattt tcataggaaa tattttatat
      121 aatacactta acaaatataa tttaaatttt tctaattatt tactgtcgat taatagattt
      181 ttttcttgca tataagtgca tatttatggt acgatttaca tggtaaaata aaaaagggga
      241 ggtgaaaaat aaatattcgt gaaaaagttt tcagtttgag gtattttatt aataatatat
      301 tttgaggtat tttataaata atatattata aataaagagt ataacctcaa taaatagtat
      361 caaagcgaat atatgggtaa ttactttaaa ataacagttt ttatgtttag attcacagtg
      421 tttattataa actaatgtgt aagaaatagg tctaatctgt gaatagatag tagtttattt
      481 atttaagttg agaattggtt gagtgaagta atatataaat ttaaaaagta aataaaaaca
      541 ttatttgaac aaaatatatg ttgctttatt cgagactcct ttaaaaaagc actaaaatgt
      601 aattacagaa gttgctacaa aagggggatt ttagtttgta aaatactatc acgtaaaaaa
      661 taagaattgc atatggcgaa ggttttttca tggtattaga tgagaatata ttaatttata
      721 actatatctg aaaatagtga atttatataa tttaaattta tgattaagaa gtgtattaat
      781 tttattccat gagaatatgt actctacaaa taaatgcgca attatcttaa taataattac
      841 aaattgtttt acaaaataat aatattatat ctttcataaa agaaagaatt tggacaaaac
      901 gtaaaaaata aaaatacaat taagaaagga tttaaaatta tgagctcaac agtaaaaaag
      961 aagggtaaat ttcctatggg attttatatc tgctcaacaa ctttctcatt tgaaagagca
     1021 gcatattatg cttcaaagta cttaatatat atattcctta caacagctat agttcatggt
     1081 ggtcttggaa tagataaagg acaagctgct ataatgcaag ccaaccttgt tgcattcaca
     1141 taccttgcac ctataattgg gggatatata tcagatagat ggataggagc tagatatact
     1201 atacctatag gtatgcttat catgggggta ggttactatc ttggttctat tgcaactaca
     1261 gtttctatgg taaatgcaat gataatctta gtttcgattg gtactgcatt ctttaaaggt
     1321 aacgtatctg cggtaaatgg acagcttttt gatagtcaag aagaacttga tacagcattt
     1381 tcagtacaat attcttttgt taatataggt tcttttattg gtactatagc agttggtatt
     1441 ctttacctta aaacatttgc aaaaaatggt gtacttggat tttctcaatg tttctttata
     1501 gcagctgtac tttgtgtaat aggtgcaata tggttcatat atggatggag attccttgga
     1561 aatgcaggaa agagaccttt taaagaaggt gtagttgctg aaaaagttga agaaaaagat
     1621 aagtctcctc ttacaagcat ggataagaag agaatttggg ctattatact tatatcattc
     1681 ttctctgtaa ttttctgggt attctggtat ctaacttatc ttgcagttta tgattatggt
     1741 gcagcatttg taaacatgaa tgttggagga tttgatgttc ctcttgcttg gtttgactca
     1801 ttaaattcac ttgtatgtat agtattagga ccagttcttg gagcattatg gtttaaactt
     1861 gctagtagac cacaaggaga tatgagttta tttaaaaaga caggtcttgg attaatattc
     1921 ttaggtcttg cattcttaat gcttgttggt gcagaatttt caagaggagt tggagctcct
     1981 gaaactgcta aagctagtat actatggata atcatgtttg gtattctttt aagtcttggt
     2041 gaaatgttat tctcaccact tggtaactct tttgtaagta agtatgcacc taagaagctt
     2101 ctaggtgttc ttatgggagt atggacattt gctactttta tagctggtaa aggatatgga
     2161 tatatatatg catttactct taaatttgat atgattaaag tatatactat aattccagta
     2221 atactatttg ttgcagcgat attattattc ttatctgata agacattaag tagacttgtt
     2281 gaggatgata aataatcagt aagtaattat tatgttttat agattaatct actgtaatta
     2341 tgt
//
LOCUS       DAMJWP010000335         2339 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.342, whole genome shotgun sequence.
ACCESSION   DAMJWP010000335 DAMJWP010000000
VERSION     DAMJWP010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.342"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(84..995)
                     /locus_tag="PC700_003538"
     CDS             complement(84..995)
                     /locus_tag="PC700_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HDF2582801.1"
                     /translation="MSRAERLIELMITINAKRSFTAGELAEEFSVSKRTILRDLQVLE
                     SVGFPLYSKVGAAGGYHVLKERILPPITFSESEVKSIFFAYQSLEYYNDLPFEQETIS
                     VLKKFLNCIPNDIQYNIENMRRKLVFWTPDRHCSTPLLKELFNIVMNEFTIKIEYSSK
                     QKNSIRTIVPIGLYAMNGLWYCPAYCIKSESVKEFRADRIVKILSIENLSNKKYKVLS
                     SIHDYLKNMEVGTDYHIKINLTDEGVKRCETEFLLARGLKILSKGGYIDMYIPKSTLN
                     WVAEYFLTFGKNATIIEPIELKHLIKK"
     gene            1106..1573
                     /locus_tag="PC700_003539"
     CDS             1106..1573
                     /locus_tag="PC700_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582802.1"
                     /translation="MENDTIKADDILNYCLSNLDDVVLMDSWGERAIYYNPNGVLKRG
                     VYVLTIKEKDSNHDKGSLVSRPNVYRVNIGLKKETFIEMFGYIPKRPGVGQIVDMDFD
                     FTKLDTIMPHPIYSWMGWICVLSPTEKTFENFKTLIEESYNFAKQKFKKRKNK"
     gene            complement(1741..>2339)
                     /locus_tag="PC700_003540"
     CDS             complement(1741..>2339)
                     /locus_tag="PC700_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582803.1"
                     /translation="KLKRTLFVGLALVLAIGITACARDNEKPNENAGKNPNESVNNNE
                     GYMNYYSTSYNDYIAGLNRYSIYDTPESINNQFKDKEYPGNEKYLSDVKAAYKDSRDK
                     IQSFVTSLKKDGKTEDTELKRMNDDLIAEGERLVKDIDERIEKLDKVSDEDMKKGQND
                     FMKLVHGTEDVGNDIGSGFRKMIKDMNDRLGITGNNKK"
BASE COUNT          789 a          391 c          278 g          881 t
ORIGIN      
        1 ttatcctatc tctacatata agtttcccta tatgctgact aatttagcta ctaactcatg
       61 actaaagtca caagtgtgcg tagctatttt ttaatcaaat gtttaagttc tataggttct
      121 attattgtag cattttttcc aaaagttaaa aaatattcag ccacccaatt aagtgtagat
      181 tttggtatat acatatctat gtatccaccc ttagacaaaa ttttcaatcc tcttgccaac
      241 aaaaattctg tttcacaacg ttttactccc tcatctgtca gattaatttt aatatgataa
      301 tctgtaccta cttccatatt cttaagataa tcatgtatag aagataaaac cttatatttc
      361 ttatttgata agttttctat actcaaaatc ttcacaattc tgtctgcacg aaattcctta
      421 acagattctg attttataca gtaagctgga caataccaaa gtccattcat agcatacaaa
      481 ccaataggaa caatggtacg tatactattt ttttgctttg atgaatattc aattttaatt
      541 gtaaattcat tcattactat attaaacaat tccttcaata atggtgttga gcaatgccta
      601 tctggtgtcc aaaaaacaag tttacgtctc atattctcaa tattatattg aatatcattt
      661 ggtatacaat ttaaaaattt ctttaaaaca gaaatagttt cttgctcaaa tggtaaatca
      721 ttataatact ctagtgactg atatgcaaag aatatagatt ttacctcact ctctgaaaat
      781 gtaattggtg gaagtatacg ttctttaagt acatgatatc ctccagccgc tccaactttt
      841 gaatatagtg gaaatccaac actttccaat acctgtaaat ctcttagtat agtacgtttt
      901 gatacagaaa attcttctgc tagctctccc gctgtaaaac ttctttttgc atttattgtt
      961 atcatcagtt caattaaacg ctcagctctt gacatattca tactcctaat ttaaatatag
     1021 tgacaataat tgtcaccatt atatgataag atattcttgc atctaaagca ataataatat
     1081 taaagaaaga agggattgat attttatgga aaatgatact attaaggctg atgatattct
     1141 caattattgt ctatcaaact tagatgatgt tgtactaatg gatagttggg gggaacgagc
     1201 aatttactac aatcctaatg gtgttttaaa gcgaggggta tatgttctta ccattaaaga
     1261 aaaagacagt aatcatgata aaggttcgtt ggttagtcgt ccaaatgtat accgtgtgaa
     1321 tataggatta aaaaaagaaa cttttattga aatgtttgga tatattccaa agcgtccagg
     1381 tgtagggcaa atagttgata tggattttga ttttacaaaa ttggacacaa tcatgccaca
     1441 tcctatctac tcatggatgg gatggatatg cgtcctaagc cctactgaaa agacatttga
     1501 gaactttaaa actttaatag aagaatctta caattttgca aaacaaaaat ttaaaaagag
     1561 aaaaaacaag taaaaacata aaaaaataac ctctaaataa actataatga aaatatgagg
     1621 ttacaagata aaaattagta tcttgataac atacaaaaaa gcgaagccaa aaaatatatt
     1681 aattacaagg cttcgctata tttcaaatat ttatttctaa ctttaaaact atttactcta
     1741 ttatttttta ttatttcctg ttattcccaa tctatcattc atatctttta tcatttttct
     1801 aaaaccacta ccaatatcgt ttccaacatc ttcagttcca tgaactagtt tcataaaatc
     1861 attttgacct tttttcatat cttcatcaga taccttatct aatttttcta ttctctcatc
     1921 tatatctttt actaatcttt ccccttcagc tattaggtca tcattcattc tctttaactc
     1981 tgtatcttct gttttaccgt cttttttaag agatgttaca aatgattgta ttttatctct
     2041 actatcttta tatgcagcct ttacatcact tagatatttt tcatttcctg gatattcttt
     2101 atctttaaac tgattgttta tactttctgg agtatcataa atagaatatc tatttagacc
     2161 tgctatataa tcattataac ttgttgaata ataattcata tatccttcat tattatttac
     2221 agattcatta ggattttttc ctgcattttc atttggtttt tcattatccc tagcacatgc
     2281 agttattcct atagctaata ctaatgctaa tcctacaaat aaagttcttt ttaatttca
//
LOCUS       DAMJWP010000336         2337 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.343, whole genome shotgun sequence.
ACCESSION   DAMJWP010000336 DAMJWP010000000
VERSION     DAMJWP010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.343"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            204..2276
                     /locus_tag="PC700_003541"
     CDS             204..2276
                     /locus_tag="PC700_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2582804.1"
                     /translation="MKKVFGVLQKVGKSLMLPVALLPAAGILLGVSNALAGDALLSHF
                     PALANPTFQLIISIMESSGQIIFNNLPLIFAVGVAVGLTEGEGVAALAAIVGFLILNT
                     SMGVMAGVTEDMVDNSMYASIVGIPTIQTGVFGGIIIGIVAAYLYKKFYKIELPQYLG
                     FFAGKRFVPIVTSLVAIAIGIILSFIWPPIQEGLLSFSKNVIEANQTLAAFMEGSLER
                     LLIPFGLHHVFHNPFWYQFGEYVNKAGELVMGDQKIFFAQLKDGVNFTAGTFMTGAFP
                     FMMFGLPAAALAMVHEAKPEKKKFVAGIMASAALTSFLTGITEPIEFAFLFVAPVLFA
                     IHCLFSGLSYAIMQLLGVKIGFTFSGGLIDFLLFGVLPNRTPWYLAILVGIGFAVLYY
                     SVFRFMIRKFNLKTPGREDESETNAEVIEMSKNELASEILIALGNKDNLKSLDACITR
                     LRLVVNDINKVDKEKLKLLGAAGVMVIGNNVQAIFGPKSDSIKSDIKDIIEGKTKVES
                     TVTKNVDKNKVKKNMEVANMENALVEKIDSLTNGEILDIEEVPDSVFSSKLMGDGFAI
                     KSSDGLIYSPVDGTIGVIFPTKHAIIIKSKKSGVEILIHLGIETVNLEGNGFEVFVNV
                     GDEVKAGEKLVKMDLEYIEKNCLSTISPVVFTNLEQNQKLSIKKGITTAKEENRVSIL
                     RYDIEKIG"
BASE COUNT          859 a          253 c          449 g          776 t
ORIGIN      
        1 aagattgtta ctgataatgc aggcaagact tatgtttata aaaaaattat aatagtttat
       61 ttttaattct gaaattaggt taatataggt ttatttatat taacataagt ttaattttag
      121 aattaagtat actaatgtaa ctttttataa acttaagtct tttttattta aaaaaatata
      181 aaataataag gagagaaaag taaatgaaaa aagtatttgg tgttttacaa aaagtaggaa
      241 aatctctaat gctaccagtt gcattacttc ctgcagcagg tatattactt ggcgtgagta
      301 atgctttagc aggagacgca ttgttgtctc attttccagc tttggcgaat cctacatttc
      361 aacttataat aagtataatg gaaagttcag gtcaaattat atttaataac ttaccactta
      421 tatttgcagt tggggtagct gttggtttaa ctgaaggaga aggtgttgca gcattggcag
      481 ccatagtagg ttttttaatt ctcaatacat ctatgggtgt tatggcgggt gttacagaag
      541 atatggtaga caattctatg tatgcaagta ttgttggaat accaacaatt caaacagggg
      601 tatttggtgg tattattata gggatagtgg ctgcttatct ttataaaaag ttttataaaa
      661 ttgaattacc tcagtatcta ggtttttttg caggtaaaag atttgtacca atcgttactt
      721 cattagttgc tatagcaata ggaattatat tgtcttttat ttggcctcca atacaagagg
      781 gattacttag tttctcaaaa aatgttatag aagcaaatca aacactagca gcatttatgg
      841 aaggttcttt ggaaagacta ttaataccat ttggtttgca tcatgttttc cataatccat
      901 tttggtatca atttggagaa tatgtaaata aagctggtga gcttgtaatg ggtgaccaaa
      961 agatattctt tgctcaactt aaagatggag taaatttcac agcaggtact tttatgacag
     1021 gtgcatttcc atttatgatg tttggtcttc cagcagcagc tcttgctatg gtgcatgaag
     1081 caaagccaga aaagaaaaaa tttgtagcag gtataatggc atcagcagca ttaacctctt
     1141 ttttaacagg aataaccgag ccaatagagt ttgcgttttt atttgttgca ccagtattat
     1201 ttgcaataca ttgtttattt tcaggtttat catacgcaat tatgcaatta ttaggagtta
     1261 aaattggatt cacattttca ggaggattaa tagatttctt attatttggt gttcttccta
     1321 atagaactcc atggtacttg gctatcttgg taggtattgg ttttgcagta ttatactata
     1381 gtgtatttag atttatgata agaaaattta atttaaagac accaggaaga gaagatgagt
     1441 cagaaactaa cgctgaagtc attgaaatgt ctaagaatga attagcatca gagattttaa
     1501 ttgctttagg aaataaagac aacttaaaga gcctagatgc gtgtataaca agacttagat
     1561 tggttgtaaa tgatataaat aaagtagata aagaaaaatt gaaattactt ggagcagcag
     1621 gtgttatggt tattggcaac aatgttcaag ctatatttgg accaaaatcg gattcaataa
     1681 agtctgatat aaaagatata atagagggaa aaacaaaggt tgaaagtact gttactaaaa
     1741 atgttgataa aaataaagtg aaaaaaaata tggaagttgc caatatggaa aatgctttag
     1801 tagaaaagat tgattcatta acaaatggag agatattaga tatagaagaa gtgccagatt
     1861 ctgtattttc aagtaaactg atgggggatg gatttgcaat taaatctagt gatggattga
     1921 tttattcacc agttgatgga actattggag ttatatttcc aactaagcat gcaataataa
     1981 taaaatccaa aaaaagtgga gtagaaatac ttatacattt agggatagaa actgtaaatt
     2041 tagaaggaaa tggatttgaa gtatttgtga atgtaggaga tgaagtaaaa gcaggagaaa
     2101 aattagttaa aatggactta gaatatattg aaaaaaattg tctatcaaca ataagtcctg
     2161 tagtatttac gaatttagaa caaaatcaga aattaagcat taaaaaagga ataactacag
     2221 caaaagagga gaacagagta agtatattaa gatatgatat tgaaaaaata ggatagctat
     2281 atgatagtat aactaaaagt tatgtgagat ttaataaata tataaatagg ttcagtt
//
LOCUS       DAMJWP010000337         2276 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.344, whole genome shotgun sequence.
ACCESSION   DAMJWP010000337 DAMJWP010000000
VERSION     DAMJWP010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.344"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            143..1360
                     /locus_tag="PC700_003542"
     CDS             143..1360
                     /locus_tag="PC700_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HDF2582805.1"
                     /translation="MLGKKCMDYLQTLGKISSTTNGLTRLILTQEHKKSIDLISSWME
                     GLNLDIEIDDIGNVIGTYKSSFPNAPTLVVASHQDSVKCGGIFDGMLGIIVPLVGLEE
                     AKHNNRSYPFNIKLIAFAEEEGTRFETSLMGSKVFAGTFKEELLKSVDENGITLEEAV
                     TKFGFNTKNLTNLHPRKDVDAYLEFHIEQGPVLENESLPAGIVSSITGFKSFKISVNG
                     KSGHAGTLPMNMRLDAGCCACECVLAIEKVAKTTADLVATVGKMNFYPSSSNVVPERA
                     EFTLDVRSCSQEILDNSVEKIFNEISHICENRKLNYTSELAFENVPVPCSNKITKIIE
                     KSFIDLNLNPFYIYSGAGHDAQEMDNITDIGMVFIRCAGGVSHNPNESVSVDDLDTAV
                     KIFLKILDNLDLK"
     gene            1522..2220
                     /locus_tag="PC700_003543"
     CDS             1522..2220
                     /locus_tag="PC700_003543"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HDF2582806.1"
                     /translation="MNEKLIGILAGMGPRSTAPFVDLIIDECQSQYGAKYDDEFPKMM
                     IYSLPTPFYIDRPINHELMKETIIDGLQKLESTGVNFIAMPCNSAHIYFKELKESINI
                     PILNIVEETVKKLPTISQKVTLFSTSSTFESTIYQNGIIHNGHEFIFKDEWQIKLNNL
                     IQNIKMDKENPHNIDIWNKLIEDVKNESIENIVIACTDLNVVLEKSYPSINIVDSSKC
                     LAESVVNKYLKLVK"
BASE COUNT          874 a          260 c          346 g          796 t
ORIGIN      
        1 tctacagact ttatgttttt atatcattat taatactaaa atagtatttt actatacata
       61 caaaattatt tgaaagttca aaaattataa gtataaaagc taatcttagc ttaaataaaa
      121 attatatttt taggaggtaa atatgttagg aaaaaaatgt atggattatc tacaaacatt
      181 aggaaaaata agttcaacaa caaatggtct tacaagactt atcttgactc aagaacataa
      241 aaaatctatt gatttaatta gttcatggat ggaaggttta aatcttgata ttgaaattga
      301 tgacattgga aacgttatag gtacatataa atcaagcttt ccaaatgctc ccacattagt
      361 agttgcatct catcaagata gtgtaaagtg tggaggtata tttgatggta tgttaggtat
      421 aatagtgcct ctagttggtc ttgaagaagc aaaacacaat aataggtctt atcctttcaa
      481 tattaagcta attgctttcg ctgaagagga aggaactaga tttgaaactt ctttaatggg
      541 aagtaaagtt tttgctggta cttttaaaga ggaacttctt aaatctgttg atgaaaatgg
      601 aattacactt gaagaagctg taacaaagtt tggatttaat acaaaaaact taacaaattt
      661 gcatcctaga aaagatgtag atgcatattt agaatttcat atcgagcagg gtcctgtttt
      721 agaaaatgaa tctcttcctg ctggaattgt atcaagtatt acaggattta aaagttttaa
      781 gatatcagta aatggtaagt ctggacatgc tggtacactt ccaatgaata tgagacttga
      841 tgctggatgt tgtgcctgtg aatgtgtatt agctatcgaa aaagtagcta aaacgactgc
      901 tgatttggtt gctactgtag gaaagatgaa tttctatcca tcttcatcaa atgtggtacc
      961 tgaaagagct gaatttacac tcgatgtacg ctcttgttca caagaaattc ttgataattc
     1021 tgtagaaaag atatttaatg aaatatcaca tatatgtgaa aatagaaaac ttaattatac
     1081 aagtgaactt gcttttgaaa atgttcctgt accatgttct aataaaataa caaaaatcat
     1141 agaaaaaagt tttatagatt taaatcttaa tcctttttat atttatagtg gtgctggcca
     1201 tgatgctcag gaaatggata atataactga tattggtatg gtatttataa gatgtgctgg
     1261 gggcgtaagt cataatccta atgaaagtgt ttctgtagat gacttggata ctgctgtaaa
     1321 aatattttta aaaatactag ataatcttga tttaaaataa tttttatata agaaagtata
     1381 ttaaaatata taatctaaaa aatcacttta tatttatatt atacaagtgt acaatataat
     1441 ctattataaa atataatttt atgcttttat agatttttat tttatacata aatatattta
     1501 tatataaagg agataatttt tatgaatgaa aaattaattg gtattttggc tggaatgggt
     1561 ccaaggtcaa cagccccttt tgttgattta attattgatg agtgtcaatc tcaatatgga
     1621 gcaaaatatg atgatgaatt tccaaagatg atgatatatt cattacctac cccattttat
     1681 attgaccgtc caataaatca tgaacttatg aaagaaacta taattgatgg attacaaaag
     1741 cttgaatcaa ctggtgtaaa ttttattgca atgccatgca attcagcaca tatatacttt
     1801 aaagaattaa aagagtctat taatatacca atattaaata ttgtagaaga aacagttaaa
     1861 aaattaccta ctatctcaca aaaagtaact cttttttcga ctagttcaac ttttgagtct
     1921 acaatttacc agaatggtat aatacataat ggacatgaat ttatatttaa agatgaatgg
     1981 caaataaaat taaacaatct tattcaaaat ataaaaatgg ataaagaaaa tccacacaat
     2041 attgatattt ggaataaact tatcgaagat gttaaaaatg aatctataga aaatatagta
     2101 attgcttgta ctgatttaaa tgtagtttta gaaaaatcat atccttcaat aaatattgtt
     2161 gattcatcaa aatgcttggc tgaatcagtt gtcaataaat atctaaaatt agttaaataa
     2221 atttttcatg cactatatta taaaaaatat atgttataag tttattttaa atagta
//
LOCUS       DAMJWP010000338         2261 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.345, whole genome shotgun sequence.
ACCESSION   DAMJWP010000338 DAMJWP010000000
VERSION     DAMJWP010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.345"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(96..1010)
                     /locus_tag="PC700_003544"
     CDS             complement(96..1010)
                     /locus_tag="PC700_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HDF2582807.1"
                     /translation="MIDNQKYVILSLELHLFFSRIMKEHALFLEAGFTNKNYNLAMEA
                     DHYKKQFEDLLSYTVSASNGIIRPDILYSEELVTTLTSVAEQKTEEFTGIEINKDITT
                     RELNLQSGVNPQVGQDLVNYVAQLNSDAIRLLDGLINFKERVLDGVLSCTIFTSNYPL
                     LLEHIIHEANLYRSYVVDLENKIDIESKNAKEIELFWDHIMMEHALFMRGLLDPSEGE
                     LINTSNDFAIKFNELIEKTNEMTDSNIKNITEETLNETVEFKDFKEAGASGIEQCKIK
                     SIILPLLADHVLREANHYIRILESYKNM"
     gene            1352..1554
                     /locus_tag="PC700_003545"
                     /pseudo
     CDS             1352..1554
                     /locus_tag="PC700_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003381593.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
     gene            complement(1842..2024)
                     /locus_tag="PC700_003546"
     CDS             complement(1842..2024)
                     /locus_tag="PC700_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582808.1"
                     /translation="MFKDKIDECVHIMTAYIASLKEYYSFIETQIGDFIKKYGEDVVE
                     LCLHRVMILLCECGLA"
BASE COUNT          827 a          281 c          283 g          870 t
ORIGIN      
        1 tttaaatttt ataagtttat aaaaaataaa ggtacagtta actgtacctt tattttatct
       61 aaatatgcaa agattatttt aaaagtaatt tttaattaca tgtttttata actctccaat
      121 attctaatat aatgatttgc ctctcttaaa acatggtctg ctaaaagtgg taatattata
      181 gattttatct tacactgttc tattcctgat gctcctgctt ctttaaaatc tttaaactca
      241 acagtttcat ttagagtttc ttctgtaatg ttcttgatat tagaatcagt catttcgttt
      301 gttttttcaa ttaattcatt aaattttata gcaaaatcat ttgaagtatt tattagttca
      361 ccttctgagg ggtctagtaa tcctctcata aacagagcat gttccatcat aatatggtcc
      421 cagaataatt ctatttcttt agcgtttttt gactcaatat ctattttatt ttcaaggtca
      481 actacataag aacgatataa atttgcttca tgtattatat gttcaagaag tagagggtag
      541 tttgatgtaa atatagtaca tgatagtaca ccatctaaga ctctttcttt aaaattaata
      601 agcccatcaa gtaatcttat tgcatcagag ttaagttgag ctacatagtt cactaagtct
      661 tgaccaactt gtgggtttac accactttgt agatttaatt ctcttgtagt gatgtctttg
      721 tttatttcta tccctgtaaa ctcttctgtt ttttgttctg caactgatgt gagagtagtt
      781 acaagctctt ctgaatataa tatatcaggt ctaattatac cattactagc actaacagtg
      841 tatgataata aatcttcaaa ttgcttttta tagtggtcag cttccatagc aagattataa
      901 tttttatttg tgaatcctgc ttctaaaaaa agagcatgct ctttcataat tcttgaaaaa
      961 aataaatgta attctagtga taaaataaca tatttttgat tatctatcat aatttcctcc
     1021 ctttagaaag tatcctattt ataatacttt attcgtaata tttaaattta tgtatattat
     1081 aaataaatta actaaattca attgctacta atatcaacag tttttattaa gttatgggcg
     1141 aaaaaattat cttactgtgt ttcttattag atatagatat aatgatttaa ggtacttgtt
     1201 ggctattatt tattaagagc acaagtaata ctagaaattt ttatgagaat tatgtaattt
     1261 ataagtatta ttttgataca aaacaaatat tttttgtgaa acaagaactt ttatagcaaa
     1321 aaatgaataa atatgaagca atcataattt tataccacaa caattattaa ttctaatagc
     1381 tgcacttaat gttttaggta tgggatttaa aaagtataaa aaattaagtg ataaatatat
     1441 tccattatat tgcttgtgct tggagtagta ttttctgtat ggatgttagg atttaatcca
     1501 acttctattt tacaggggat tctatgctgg ggtgtttcaa ttggattaaa ttaagtatgc
     1561 aaacaatttc aagacaatga taaataattg gatgtgtgat aggaatatca acaaaaaaca
     1621 ttattaaaaa gaattatttt atttataaaa taaaacaagt aataagaata aataaggagc
     1681 aactaaaatg atttaaatat actagaatta aaaagagaat gtaaagttgt ctagtaatgg
     1741 aggtaaaata ttttaaacga taagctatga aattgatata ttaaaatggg taagaagaat
     1801 attatatagg attcttctta cccattttaa taaatttaat tttatgccaa accacattcg
     1861 catagtagaa tcatcactct atggagacat aactctacaa catcttctcc atatttttta
     1921 ataaaatcac caatttgagt ttctatgaat gaatagtatt cttttaaact agcaatatat
     1981 gcagtcatta tatgaacaca ttcatcaatc ttatctttaa acacaactaa tccccctaaa
     2041 aaacgaaaaa taaaaatatt cagtaaatag cagatattat ttcactcata aatctatata
     2101 gtatgatata ttgcttagat atattttaca agtggatatg taaaactggt tattataatc
     2161 gcaaaactgg ataaactttc attcttaaaa gaaaatttat ccagtttaaa gtttaattat
     2221 gaatatttga gttagtagat atttaatata aattaatata a
//
LOCUS       DAMJWP010000339         2259 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.346, whole genome shotgun sequence.
ACCESSION   DAMJWP010000339 DAMJWP010000000
VERSION     DAMJWP010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.346"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(512..763)
                     /locus_tag="PC700_003547"
     CDS             complement(512..763)
                     /locus_tag="PC700_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582809.1"
                     /translation="MSSYFITRGDVMKILQQFKPYKYDKEKNIEYDEKIIANQNTEVY
                     IVYPRISDEENRRRWEEFNEVAKEVAINIAMKKVETVES"
     gene            complement(1009..1164)
                     /locus_tag="PC700_003548"
     CDS             complement(1009..1164)
                     /locus_tag="PC700_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HDF2582810.1"
                     /translation="MALDVKIEVKIIKDVVILNIIHWKKETSLLTVKIARGQGDLSYV
                     KYFTNES"
BASE COUNT          755 a          328 c          201 g          975 t
ORIGIN      
        1 aatttattaa atttttgcat taaaaaaact agaaattaat ttaagtcttg aattacttat
       61 aactttctac gtatctacct ttatattctt actctgattt tatatctaat attaactctt
      121 catcaaagct attaaatata cctctaattg agtcaattat ttcacttact tcacctttaa
      181 tttttatatt aacttcaacc atttcaaatc tcatcttttt atcttataat ttctataagt
      241 ttttttattt attgtgttgc ttactttgac cagcactttt ttatttaata gtctaaatga
      301 tattttttac cagttttaat atccttaaat actatattta ctaaaaactt ataatttctt
      361 ttaatacatt cttattacta ataacaatac tcaacaattt tatcgccttt tttcaaatat
      421 gatttaactt ttttattcct aattatttaa aaatagttgt ccaattttct tctatgagaa
      481 agtgctaagt attagcaact tctctattct cttaactttc tactgtctca actttcttca
      541 tagctatatt aatagctact tctttagcta cttcattgaa ctcttcccat cttctcctat
      601 tttcttcatc agatatacgt ggatatacta tataaacttc tgtgttttga ttagctataa
      661 ttttttcatc atattctata tttttttctt tatcatattt gtatggttta aattgttgta
      721 gtattttcat aacatcaccc cttgttataa aatatgaact cataaacttg tcttatactt
      781 atttaattgt tacaattttt acttaaaatc actttatatt ctatatttta aagctaaatc
      841 ctttacaaaa gtaacatatt cttctataag tttcttatcc tcttatatag tatctagttt
      901 gtttaactta tctcttttaa acttacatac gccttcttct gccattcgtc ttttattaat
      961 tgttaacatt gtttcaagag acacaccaaa actcttgtta agaagctctt agctctcatt
     1021 agtaaaatat tttacataac ttaaatcacc ttgacctcta gcaattttaa ctgttaatag
     1081 acttgtttct tttttccaat gtattatatt taatataaca acatctttta ttattttaac
     1141 ttctattttt acatctaatg ccatattatt gctttattaa tttgtatcct taaatcttaa
     1201 attttctatt aatacatctt ctatacagct tagtttctct cgcttaatct tgaaatatat
     1261 tttctctaaa ttgtcaaatt gttcccaagt tttatcaata tctaatattt tgcagtgtct
     1321 atttgcacat ctttcgatcc aaaggtataa ttgactagaa tgttttgaaa tcaactgact
     1381 attagtaggt tagttcttaa attctttcaa atactcgaat tgtaataaaa cataatttac
     1441 attctcttta tttctattga agttagttta tatattgttt acatcatttt catatatatc
     1501 tgctagttgc tatgcattta aaattctctt attatttctt tatattactt gtaaattatt
     1561 catcctcatc atcctcttaa ttaaatttct atcttagaaa tttaatttgt aaaaaaattt
     1621 tctatatcat aatcaagtat cttagatagt ttataggtaa catatcaact ctaattgaat
     1681 attacccctt cctgtactta aaataagttg atgcattttt aaaacctaac tcttttgcaa
     1741 cactttgtaa gtataaattt tatgtatgtc ttctttttta taaaatcata gtgtatcttt
     1801 ttcatatatt caccttaatt gctatatttc aataacaata tttccaaaac agaaattttt
     1861 cgaggtaatt atgtatagat gtaaatcaaa gaattaaata tactagggaa aatatgaata
     1921 gttctcaaaa agaattgctt gaaaaaacaa atataaatac aagtgttatg aatagaatct
     1981 ggtgaaagac caacaagaga cgatgggtta ataatatttg ctaaaatttt tggtttatat
     2041 actgattatg ttcttggttt atcagataca gaaaaactaa atattggcaa ttaaaactga
     2101 attgattgta ttatatgtgt gatgaatata aatatagaat tttttgagaa gcttgaaata
     2161 gcttagaaaa aataagagat tcactcaaga ataagttact aaagattaaa aaataacaaa
     2221 agtatctact aaaggatatg aaaatggttt aagagagtt
//
LOCUS       DAMJWP010000340         2228 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.347, whole genome shotgun sequence.
ACCESSION   DAMJWP010000340 DAMJWP010000000
VERSION     DAMJWP010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.347"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            41..1492
                     /locus_tag="PC700_003549"
     CDS             41..1492
                     /locus_tag="PC700_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HDF2582811.1"
                     /translation="MNFLNGILTSINNAVVFVNNYLWSYILIIMLVVIGIYFTIKTNF
                     VQFRYFIEMFRLLGDGTANKNAKKEGKISSFQAFCISTSSRVGTGNIAGIAIAVVAGG
                     PGAVFWMWLIALIGSASSFVESTLAQIYKVKDGQAFRGGPAYYMEQGLNKKWMGILFS
                     ILITICYGFVFNAVQANTVSLAFNNAFGISKMAMGIGLGILTAIVIFGGVHRVAKVSE
                     IIVPVFAGLYILVALAIVVMNITEIPSVIALIVESAFDFKGMAIGTFMGVVMTGVKRG
                     LFSNEAGMGSAPNAAATAHVTHPVKQGLIQSLGVFTDTIIICSCTAFMVLLYSGYSSS
                     GATGIELAQEALTHHIGPVGNIFIAVCIFLFAFSSIVGNYYYGESNMEFMSGSKTKLN
                     VFRVFVVGMVLFGSLTQVDVVWNLADLFMALMAIINLIAIALLGKYAFIALKDYTSQK
                     KSGIKDPVFIADEIEGLEKVSEWHRELSEEHLG"
     gene            complement(1712..2173)
                     /locus_tag="PC700_003550"
     CDS             complement(1712..2173)
                     /locus_tag="PC700_003550"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HDF2582812.1"
                     /translation="MIYEGKLIGKDLKIGIINSRFNEFITSKLLSGAEDCLLRHDVSP
                     ENIEIVWVPGAFEIPLVAQKMAKSGRYDAIICLGCVIRGATSHYDYVCSEVSKGIAKV
                     SLDSELPVIFGIVTTENIEQAIERAGTKAGNKGYDCAMNALEMANLFKSLN"
BASE COUNT          750 a          264 c          391 g          823 t
ORIGIN      
        1 tattgagaaa acaaaactaa ataggggggt aattaatata atgaattttt taaatggtat
       61 tttaacgagt attaacaatg cagtagtatt tgtaaacaat tacttatggt cgtatatctt
      121 aattataatg ttagttgtaa ttggtattta ctttactatt aaaacaaatt ttgtacaatt
      181 cagatatttt atagaaatgt ttagacttct gggtgatggt acagcgaata aaaatgcaaa
      241 aaaggaagga aaaatctctt catttcaagc attttgtata agtacatctt caagagttgg
      301 aactggaaat atagctggaa tagcaattgc agtggtagca gggggacctg gtgctgtatt
      361 ttggatgtgg cttatagcat taattggttc tgcatcaagc tttgttgaga gtacattagc
      421 tcagatttac aaagtgaaag atggtcaagc ttttagaggt ggaccagcgt attatatgga
      481 acaaggtcta aataaaaaat ggatgggtat tttattctca atacttataa ctatatgtta
      541 tggttttgta tttaatgcag ttcaagcaaa tactgtttca cttgcattta ataatgcatt
      601 tggaataagt aaaatggcta tgggaatagg tcttggaatt ttaactgcaa tagttatttt
      661 tggtggagtg catagagttg caaaagtttc tgaaataata gttcctgtat ttgcaggatt
      721 atatatatta gttgctttgg ctatagttgt aatgaatata acagagatac caagtgttat
      781 agcattgata gttgaaagtg catttgattt taaaggaatg gcgattggta catttatggg
      841 agttgtaatg actggagtga agagaggtct attttctaat gaagcaggta tgggtagtgc
      901 tcctaatgct gctgcaacag cacatgtaac tcatcctgta aaacaaggtt taattcaatc
      961 tttaggtgtt tttacagata ctataataat atgtagttgt acagcattca tggtattatt
     1021 atattcaggt tatagtagta gtggagcaac tggaattgaa ttagctcaag aagctttaac
     1081 tcatcatata ggaccagttg gaaatatatt tatagcagtt tgtatattct tgtttgcatt
     1141 tagttctata gttggtaatt actattatgg tgaatcaaat atggagttta tgagtggaag
     1201 taagactaaa cttaatgtat ttagagtttt tgttgtaggc atggttttat ttggttcact
     1261 tactcaagta gatgtagttt ggaatcttgc agacttattt atggcattga tggcgataat
     1321 aaacttaata gcaatagcac ttttaggaaa atatgcattt attgctttaa aagattatac
     1381 aagtcaaaaa aaatcaggaa taaaagaccc tgtgtttatt gcagatgaaa tagaaggcct
     1441 agaaaaggta tcagagtggc atagggaatt atctgaggaa catttaggat aaaaaatagt
     1501 ataaaattat aaaaccattt tgaaagacta gagtatgtat atgctttata tgaatataat
     1561 attgaattaa tttatattac tggaattttt aattccagta atattttttt attatataat
     1621 ttagctttat taaaagtttt tattattaaa aatagcttat ttatcgtaaa gtgaatgata
     1681 ttggtttttt aattattatg aatgttggta tttaatttaa agacttaaat aaatttgcca
     1741 tttctagcgc attcatagca caatcataac ctttatttcc agcttttgta cctgctcttt
     1801 caatagcttg ttcaatattt tctgtagtta cgattccaaa tataacaggt aattcactgt
     1861 ctaaagatac ttttgctatc cctttagaga cttcactaca tacataatca taatgagaag
     1921 tagcccctct tataacacag cctaaacaga ttatagcatc atacctacca cttttagcca
     1981 ttttttgagc aacaagagga atctcaaaag ctccaggtac ccaaactatt tctatatttt
     2041 ctggagaaac gtcatgtctt aaaagacagt cttcagctcc tgataaaagt tttgatgtta
     2101 taaattcatt aaaacgagaa tttattatac ctatttttaa atcttttcca ataagttttc
     2161 cttcatatat catttttatt tcctccaatt ttatttatgt tatagattta tatttataaa
     2221 tatcttta
//
LOCUS       DAMJWP010000341         2225 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.348, whole genome shotgun sequence.
ACCESSION   DAMJWP010000341 DAMJWP010000000
VERSION     DAMJWP010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.348"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(130..1422)
                     /gene="brnQ"
                     /locus_tag="PC700_003551"
     CDS             complement(130..1422)
                     /gene="brnQ"
                     /locus_tag="PC700_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDF2582813.1"
                     /translation="MGEKQINFKDVIVIGFALFAMFFGAGNLIFPPYLGVLSGSSWLI
                     AFIGFLFADGGLALLAVIAATKFNGDTSKMFSRAGKGLSIALGCAMVICIGPLLAIPR
                     TAATTYEMGILPTIGSGISPVIFSIVFFALVLVLTIRPSKVVDIVGSILTPALLIALA
                     VLIVKGIVSPLGEIRDSSLIQNVFAEGITQGYQTMDALAASVFASIIIMSVIAKGYTG
                     EKEKMKATISAGVIAVIGMALVYGGLCYLGATVSQQYGQDVQQTALIVAITAALLGTT
                     GKILLAIIVALACLTTAIGLSSAAGQYFSTLTDGKLKYEHIVIVVCVFSAIISNFGVS
                     TIIKFSSPILSMVYPATITLVILALFSNKIKNDNVFKCAAYMALLVSVLTVATSFGVK
                     IPLVNSLPFASLGFNWIVPVVIAGIIGNFIPSKSSKTA"
BASE COUNT          749 a          376 c          244 g          856 t
ORIGIN      
        1 aaaaataaat ttatcaaatg agattaataa tgttcttttc tccacaataa tttaataatt
       61 cttttaaata taaaaaggaa ttgccatata atttaaaggc aattccacaa ttatcaatta
      121 gttttattat tatgcagttt tactgctttt agatggaata aagtttccta ttattcctgc
      181 tattacaact ggaactatcc agttaaatcc taatgaagca aatggtaagc tattaactag
      241 aggaattttt acaccaaaac ttgttgcaac tgttaataca cttactaata atgccatgta
      301 tgcagcacat ttgaacacgt tgtcattttt aattttatta ctaaataatg ctagtataac
      361 tagagttata gttgctggat ataccatact tagtatagga gaagagaatt ttattattgt
      421 acttactcca aagtttgata tgattgcact aaatacacaa actactataa ctatatgctc
      481 atactttaat tttccatctg taagtgttga gaaatattga ccagctgctg aagaaagacc
      541 tatagctgtt gttaaacatg ctaatgcaac tataatagcc aataaaattt tacctgtagt
      601 tccaagtaaa gctgctgtta ttgcaactat taaagctgtt tgttgaacat cttgtccata
      661 ttgttgagac acagttgctc caagataaca taatccacca taaactaatg ccattcctat
      721 tactgcaatt acacctgcac ttattgtggc tttcatcttt tctttctcac ctgtataacc
      781 tttagctatt actgacatga ttataattga tgcaaataca ctagctgcca atgcatccat
      841 tgtttgataa ccttgagtta taccttctgc aaatacattt tgaattaaag aactgtctct
      901 tatttctcct aatggagata cgataccttt aactattaat actgctaaag ctataagtaa
      961 agctggagtt aatattgacc caactatatc tacaactttt gatggtctta ttgttaaaac
     1021 caatactaga gcaaagaata ctatagagaa tattactgga cttattccag aacctattgt
     1081 aggcaatata cccatttcat aagttgttgc tgctgttcta ggtattgcta aaagtggccc
     1141 tatacagatt accattgcac atcctaatgc aatacttaat cctttgcctg ctcttgagaa
     1201 catcttcgat gtgtctccat tgaacttagt tgctgctata actgctaata atgccaaacc
     1261 accatctgca aaaaggaatc ctatgaaagc tattagccaa ctacttccag ataaaactcc
     1321 tagatatgga gggaagatta agttccctgc accaaaaaac attgcaaata gtgcaaatcc
     1381 aattacaatt acatctttaa aattgatttg tttctcaccc atttaaagca cccccatttg
     1441 aattttacta ccttttacta aaaaataagt ccaatctctt cattaaatga aaaccttttg
     1501 acgagatttt atttaattat acatttttga cttaaatttg tcaattatgt ttcataattt
     1561 ataaaatctt cacaatattt aatacaactt tcttaattaa acaaaatcaa cctgtatact
     1621 ttaaacatag ttggtttttt gttttttctc taattttcct tgataaaatt aactattcag
     1681 aacaattaaa atattaagac tttttagatt attcgtttta ttattaagtt gtattgcttt
     1741 atatactgta tatatcactc tcctttttac attcatataa tctttttaat tcaatttaaa
     1801 ttttaaatat tttgtgttta ttatataatc tgaaaaaaaa gcagtcaata gcatttttaa
     1861 tttttttaat tttcaaaatt atgattctta aaaaatcgtt ttccttttat cataacacct
     1921 attaacgtta attcttgtag tattttgtat ttattttgct aaattgacag ttaactttat
     1981 tcaattttta ttctactcac gtagatttac ctcttgtctt ttagtcttaa aaccattttt
     2041 tctgaaaatt ccttattatg atttatcaac ttatttttct tcatagcaat tactttctaa
     2101 cttccaactt acttaaactt tgcagttgct agtattcacg tttacatact tatatgtcat
     2161 aattaactaa aaattcttaa tatatctctc aatgaaaaca atttactcat ttatttaata
     2221 cattt
//
LOCUS       DAMJWP010000342         2210 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.349, whole genome shotgun sequence.
ACCESSION   DAMJWP010000342 DAMJWP010000000
VERSION     DAMJWP010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.349"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            133..2196
                     /locus_tag="PC700_003552"
     CDS             133..2196
                     /locus_tag="PC700_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HDF2582814.1"
                     /translation="MKAGEIEFLSYLEGSNKSFVVPVYQRNYNWKKEQCKRLFDDLED
                     IIDSGFRTHFLGSVVSIYSMGKEYLIIDGQQRVTTVSILLIAMCQIIKEFGESKGIIT
                     TEEQIKECYLINKHSRDKDGKIKLKPIAEDRKAYYELFEEEMNTDRNSNIVSNYLYFY
                     NRIKEDNISIDDLFDAIQKLTIVEIELKNGEDDPQLIFESLNSTGLDLSEADRVRNFV
                     LMGRSSKLQEEFYNNYWYKIEKNTSSNVSSFIRDYLTIKERSIPNKNKVYFSFKRYVS
                     DNGIDIEILLKDLLKFSNYYREISYSCTDDNDVNKVLKRINKLEIFVSYPFILELFND
                     YNEGIINKKVLVESLNLVECFILRRIVCDVPPNALNKVFMNLGREIKKFSDYKENYSN
                     ILRYIFINKKSSQRFPTDSEFSNAFETKDIYNTKSKNKLYILERLENYNNKEIVDLEN
                     LINNNMLNIEHIMPQTLTNKWKESLGDNYKEIHDKYLHTIGNLTLTGYNSKLSNKTFE
                     EKKEMEDGFKDSRLYLNKYISSIDKWNEEEIKNRTKILLDRAVEIWKYPSVTYEPPSN
                     SENIFTLEDDDVNFTNESIVSFSILGESYKAKNWTEFYEQVALTLYDLDSVRFNSIVD
                     KTYKRDFIDNIKSRDVYKLRKGIKISENIYLETNLNTEAKLNIIRFLLNEFDIELNEV
                     IFSIK"
BASE COUNT          966 a          169 c          362 g          713 t
ORIGIN      
        1 aataaaataa aaataggtat aaaaaggtat ataataaggc tttcatatac atttatgagt
       61 caaaattttg atataatata tagatacatg gagttatgaa tggaatttat ttaacttata
      121 gggggagtta atatgaaagc aggagaaatt gaatttctta gttatttaga ggggtcaaac
      181 aaaagttttg tagtgcctgt atatcaaaga aattataact ggaagaaaga acaatgtaaa
      241 agattgtttg atgatttaga ggatataata gattctggat ttagaactca tttcttaggg
      301 tcagttgtat ctatatatag tatgggaaaa gaatatttaa taatagatgg acaacaaaga
      361 gtgactactg tgtcaatatt attgattgcc atgtgtcaaa taataaaaga gtttggagaa
      421 tcaaaaggaa ttattacaac agaagaacag attaaggagt gttatcttat aaacaagcac
      481 tcaagagata aagatggaaa aataaagtta aaaccaatag cagaagatag aaaagcttac
      541 tatgaactat ttgaagaaga aatgaataca gatagaaatt ctaatatagt ttcaaattat
      601 ttatattttt ataatagaat aaaagaagat aatatatcaa tagatgattt gtttgatgct
      661 atacagaaat taactatagt agaaatagag ttaaaaaatg gtgaagatga ccctcaatta
      721 atctttgaaa gtctaaattc aactggctta gatttaagtg aagctgatag agtaagaaac
      781 tttgtgttaa tgggtagaag ctctaagtta caagaggaat tttataataa ttattggtat
      841 aaaattgaaa aaaatacaag tagcaacgta agtagcttta taagagacta tttgactata
      901 aaggagagaa gtataccgaa taaaaataaa gtatatttta gttttaaaag gtatgtatca
      961 gataatggta tagatataga aattttatta aaagatttac ttaaattttc taactattat
     1021 agagagatat cttattcatg tactgatgat aatgatgtaa acaaagtttt gaaaaggata
     1081 aacaaacttg aaatatttgt atcatatccg tttatattag aattatttaa tgattataat
     1141 gaaggtataa taaataaaaa agtacttgta gaatccctta atttagtaga atgttttata
     1201 ttaagaagaa tagtatgtga tgtacctccg aatgcactga ataaagtatt tatgaactta
     1261 ggaagggaaa ttaaaaaatt tagtgattat aaagaaaact attctaatat attaagatat
     1321 atttttataa ataagaaatc atctcaaaga tttcctactg acagtgaatt ttcaaatgct
     1381 tttgaaacta aagatatata taatactaag tcaaagaata agttatatat acttgagagg
     1441 ttagagaatt ataataataa agaaattgtt gatttagaaa atttaatcaa taataatatg
     1501 ctaaatatag agcatataat gccacagaca ttaacaaata aatggaagga atcactagga
     1561 gataactata aggagataca tgataaatat ttacatacaa ttggaaattt aactttgaca
     1621 ggatataatt caaaactaag taataaaaca tttgaagaaa agaaagaaat ggaagatgga
     1681 tttaaagata gtaggcttta tctaaataaa tatatttcat ctatagataa atggaatgaa
     1741 gaagaaataa aaaatagaac taagatatta ctagatagag ctgtagaaat ttggaaatat
     1801 ccaagtgtta catatgaacc tccaagtaac agtgaaaata tatttacgtt agaggatgat
     1861 gatgttaatt ttacaaatga aagtatagta tcatttagta tattaggaga gtcatataag
     1921 gctaaaaatt ggacagaatt ttatgaacaa gttgctctta ctttatatga tttggattct
     1981 gtaaggttta acagtattgt agacaagact tataaaagag attttataga taatattaag
     2041 tctagagatg tatataaatt aagaaaaggt attaaaatat cagaaaatat atatttagaa
     2101 acaaatttaa atacagaggc taaattaaat ataattagat ttttacttaa tgagtttgat
     2161 atagagttaa atgaagtaat attttccata aaataatgaa atatatgcat
//
LOCUS       DAMJWP010000343         2176 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.350, whole genome shotgun sequence.
ACCESSION   DAMJWP010000343 DAMJWP010000000
VERSION     DAMJWP010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.350"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            101..1006
                     /locus_tag="PC700_003553"
     CDS             101..1006
                     /locus_tag="PC700_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HDF2582815.1"
                     /translation="MFFKDIELNSKKELDPYFDLVDYEACEYCFSTLYMWQHVYKTGY
                     YIGEDFAVLVGEYEGDSFSILPLAKKDKLPEVVDFVLEYFSKNNKKIYLRGITTEVVE
                     FLKEKYPGRFEYIEERDLFDYIYDAESLRTLAGKKNQKKRNHINYFLKEYAGRYEAKL
                     LDKENFDECLVLMKEWESNKEENNEFDESMDDELIGIKKIFNHYDILKDKVKVFGVYV
                     DGKLEAFSIGELLNPNMALIHIEKANPDIRGLYPFINQQFLVSEFKDVEFVNREEDLG
                     IEGLRKAKLSYHPCRFVEKYSVREA"
     gene            1015..>2176
                     /locus_tag="PC700_003554"
     CDS             1015..>2176
                     /locus_tag="PC700_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2582816.1"
                     /translation="MEIRYAKEEEIESIKEIWSYCFNDTESFMKYYFNDKYKSENTVV
                     ALDEGKIISSLQLNQYKLLLNSKVYNTSYVVGVSTLPEGRGAGYMNKVMKFTLNELYK
                     KGQLVSILMPIDYRLYRRFGYEHCYDQIEYTINTDDLKNFKSSGKMIKSNLSQIDDLI
                     RIDRTFLNEVNGNVLKDEHYYENLFKEIQSEDGFLYIHEGNEKDGYIVYFLQEDKMFV
                     RELFYKNIDALKSMLKFIYNHNTQCKIVTISTPTIDKIRFILDNPKDSDIKIKPFMMG
                     RVINVKKFIEDIDIEKDINSSFNLLIEDKFIDENNGLFKISIQNKKVSVEQLDKKGAE
                     KPQEDFDIKLDINTLTQLSFSYIDVNEAIFLNDIKDVSEETLETLNCIFSKKN"
BASE COUNT          894 a          183 c          382 g          717 t
ORIGIN      
        1 aacaatataa atgaaattaa taccataata aatttggagt tttttatata tacaaaataa
       61 aatttttaaa attaagcaat aaaaaaggta ggaggatttt atgtttttca aagatattga
      121 actaaattca aaaaaagagt tagacccata ttttgattta gtagattatg aagcttgtga
      181 gtactgtttt agtactttat atatgtggca acatgtttat aagacaggat attatatagg
      241 tgaagatttt gccgttttag ttggggaata cgaaggagat agtttctcaa tattaccact
      301 tgctaaaaaa gataaacttc cagaagttgt agattttgtc cttgaatatt tttctaaaaa
      361 taataaaaaa atatacttaa gaggtattac tactgaagta gtagagtttt taaaagaaaa
      421 atatccaggt agatttgagt atatagaaga aagagattta tttgactata tttatgatgc
      481 agaaagttta agaactctag ctggtaaaaa gaatcaaaag aagagaaacc atataaacta
      541 tttcttaaaa gagtatgcag gtagatatga agctaaatta ttagataaag aaaactttga
      601 cgagtgttta gtactaatga aggaatggga aagtaataag gaagaaaata atgaatttga
      661 tgaaagtatg gatgacgaat taataggtat caaaaagata tttaaccatt atgacatatt
      721 aaaagataag gtaaaagtgt ttggtgttta tgtggatgga aaattagagg cttttagtat
      781 aggagaactt ttaaatccaa atatggcatt gattcatata gaaaaagcta atcccgatat
      841 aagaggtctg tatccattta taaatcaaca atttttggta agtgaattta aggacgttga
      901 gtttgtaaat agagaagaag atttaggtat tgaaggatta agaaaagcta aactttctta
      961 tcatccttgc agatttgttg aaaagtacag tgttagggag gcttaataga ctatatggag
     1021 attaggtatg ctaaagaaga agagatagaa agtataaaag agatttggag ttattgtttt
     1081 aacgatacgg agagttttat gaagtactat tttaatgata aatataaatc tgaaaatact
     1141 gtagtagctt tagatgaagg aaaaattatt tcttctcttc agctaaatca gtataagtta
     1201 ttactaaata gcaaggtata taatacatca tatgtagtag gagtttctac acttccagag
     1261 ggaagaggag ctggatatat gaacaaagta atgaaattta ctttaaacga attgtataaa
     1321 aaagggcaat tagtttctat attgatgcct attgactata gactttatag aagatttgga
     1381 tatgagcatt gttacgacca gattgaatat actattaaca cagatgattt aaaaaatttt
     1441 aagtcaagtg gaaaaatgat aaagtctaat ttatcacaaa ttgatgattt gatacgaata
     1501 gatagaactt ttttaaatga ggttaatggt aatgtgttaa aagatgaaca ttattatgaa
     1561 aacttattta aagagattca aagtgaagat ggatttttat acattcatga aggaaatgaa
     1621 aaagatggat atatagttta ttttcttcag gaagataaaa tgtttgtcag agagttattt
     1681 tataaaaata tagatgccct taaatcaatg cttaaattta tatataatca caatactcaa
     1741 tgtaagatag ttactatatc tactcctaca atagataaga taagatttat attagacaac
     1801 cctaaagata gtgatataaa aataaaacct tttatgatgg gaagagttat aaatgtaaag
     1861 aagtttattg aagatataga tattgagaag gatattaata gctcatttaa tttattaata
     1921 gaagacaagt ttatagatga aaataatggt ctatttaaaa tttctataca gaataagaag
     1981 gtatctgtag agcagttaga taaaaaaggt gcagaaaagc ctcaagaaga ttttgatatt
     2041 aaattagata taaatacctt aacacagtta tcattttctt atatagatgt aaatgaagct
     2101 attttcttaa atgatataaa agatgtttca gaagaaacac tagaaacact taattgtatt
     2161 ttttctaaaa aaaata
//
LOCUS       DAMJWP010000344         2167 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.351, whole genome shotgun sequence.
ACCESSION   DAMJWP010000344 DAMJWP010000000
VERSION     DAMJWP010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.351"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(<1..233)
                     /locus_tag="PC700_003555"
     CDS             complement(<1..233)
                     /locus_tag="PC700_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HDF2582817.1"
                     /translation="MNYYELLRIKKERHKNKNFLIIDGEKYTYENILSYSEQLGKQIS
                     LGENISILIYSKNIMFQLISFFAINYSKNIPII"
     gene            complement(237..1373)
                     /locus_tag="PC700_003556"
     CDS             complement(237..1373)
                     /locus_tag="PC700_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HDF2582818.1"
                     /translation="MKEVFILGGLRSHIGLKNGIFQFVQPELLGASVLKDLIKKYEID
                     KIDEIICGNAVGTGGNIARLMTLTAGVSNEVPAFTVDMQCASAMMSIDIAFSKVKSGQ
                     CNLIIAGGFESSSLQPMRTYHKNDKRYNTNNPNYTVAQFSPDDNSQNSMLEGAERVAE
                     LYQIERADLDFWVKESHKRAKEAREEKILEDIISPINNSTYDEGIRDKMSQRLLDRMP
                     PILGKETITNAANSCLINDGASFIIICSKKYLEHVKKKPKTKIINTCTIGTDATLSPT
                     SAIQAMDKLLEIESLNYLDVSAVEFNEAFAVIDVLFQRKYPELIERYNIFGGALAYGH
                     PYGASGAIITLHLLKALEKTKGRYGICSIAAAGGLGSALLLERV"
     gene            complement(1405..1950)
                     /locus_tag="PC700_003557"
     CDS             complement(1405..1950)
                     /locus_tag="PC700_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HDF2582819.1"
                     /translation="MKNTKVNIQDLTKMSICVALLCISSYIYIPLPFTPAGVTAQTIM
                     INLIALILTPRQAFSTVGVYIMIGLIGIPVFGGGTSGIGKLLSPTGGFYFGFLFAALI
                     ISLLKGRNNDIKRYILITIFVGMPIIYFMGTVFMCYFNQMTVEAALFAAVVPFLIGDT
                     IKSVIASFLGTKLNNVLRRNL"
BASE COUNT          711 a          395 c          235 g          826 t
ORIGIN      
        1 caaataatag gaatattttt tgaataatta atagcaaaaa aactaatcaa ctgaaacatt
       61 atatttttag aatatattaa aattgagata ttttcaccta aagaaatttg ttttcctagt
      121 tgctcactat aacttaatat attttcataa gtatattttt ctccatctat aattaaaaaa
      181 tttttatttt tatgcctctc tttttttatt cttaaaagtt cataatagtt cataatctac
      241 accctttcaa gcagtaaagc tgaacctaga ccaccagctg cagctataga gcaaattcca
      301 tatctaccct tagttttttc taatgctttt aaaagatgta atgtaataat tgcacctgaa
      361 gcaccatatg gatgtccata cgctaaagct ccaccaaata tattatatct ctctattaac
      421 tctggatact ttctttgaaa caacacatct ataactgcaa atgcttcatt aaattcaaca
      481 gcagatacat ctaaataatt aaggctttct atctctaaaa gtttatccat tgcttgtatc
      541 gctgatgttg gacttaaggt tgcatctgtt cctattgtgc atgtattaat aatttttgtt
      601 ttaggcttct tttttacatg ttctaaatac ttcttagaac atattataat aaatgaagct
      661 ccatcattaa taagacatga atttgctgca tttgtaatag tttcttttcc taaaattggt
      721 ggcatcctat ctaataacct ttgactcatc ttatcacgta taccttcatc ataggtacta
      781 ttatttatag gagatataat atcctcaagt attttttctt ctcttgcttc ttttgctctt
      841 ttatgacttt cctttaccca aaaatccaag tctgctctct caatttgata taactctgct
      901 actctttccg ccccttcgag catactattt tgactattat catcaggaga aaattgagca
      961 actgtataat ttggattatt tgtattatat cgcttatcat tcttatgata tgtgcgcatt
     1021 ggttgtagag aactactttc aaaaccacct gcaataatta aattacactg accagattta
     1081 acctttgaaa aagctatgtc tatactcatc atagcagaag cacactgcat atccacagta
     1141 aaagcaggta cttcatttga cactcctgct gttaaagtca ttaatctggc tatattccct
     1201 cctgtcccaa ctgcatttcc acatataatc tcatcaattt tgtcaatttc atacttttta
     1261 attaaatctt ttaaaacact tgctcctagt aattcaggtt gaacaaattg aaaaattcca
     1321 ttttttagac ctatatgact tctcaatcct cctaatataa aaacttcctt catacattac
     1381 acccttcctt atattataga tttatcatag atttcttctg agtacattgt tcagtttagt
     1441 gcctaaaaaa gatgcaataa ccgactttat tgtatctcca attaaaaatg gtactactgc
     1501 tgcaaaaagt gctgcttcta ctgtcatttg attaaaatag cacataaata ctgttcccat
     1561 aaaatatata ataggcattc caacaaaaat tgttattagt atatatctct taatatcatt
     1621 atttcgtcct tttaataaac taataattag tgctgcaaac aaaaacccaa aataaaaacc
     1681 acctgttggg cttaacaact ttccaatacc agatgtacct ccaccaaata ctggaatccc
     1741 aattaaacct atcattatat atacaccaac tgtactaaat gcttgtcttg gtgttaaaat
     1801 caaagctatc aaatttatca ttattgtttg tgctgttact cctgctggag taaatggcaa
     1861 tggtatatat atgtaagatg atatacacaa taatgctaca catattgaca ttttagttaa
     1921 atcttgaata tttactttag tatttttcat ttttataatt cccttcatat gtcttatttc
     1981 atttagttta tatgtacata tacaaagcct ctattaaagc tttgttttag tataaaataa
     2041 agacgttttt ttgtcaacca ttacaagtat ttaggtttac gtttaattta taagtaaact
     2101 ttatactgtt actttatatt ttaatagtca tttataaaac tttgcttaaa aacagctcta
     2161 tttagac
//
LOCUS       DAMJWP010000345         2159 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.352, whole genome shotgun sequence.
ACCESSION   DAMJWP010000345 DAMJWP010000000
VERSION     DAMJWP010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.352"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            83..>2159
                     /locus_tag="PC700_003558"
     rRNA            83..>2159
                     /locus_tag="PC700_003558"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          645 a          428 c          610 g          476 t
ORIGIN      
        1 atctaatttg taatatataa agctgataac tttttaaaat tatcgaagtt gatagcttct
       61 aatctatcaa acctttttaa ctggtcaagt tattaagggt gcagggcgga tgccttggca
      121 ctaggagccg atgaaggacg tgataagctg cgataagctt cggggagttg cacgtaaact
      181 ttgatccgaa gatttccgaa tgaggaaact cacttagagt aatgtctaag tatcattaag
      241 tgaatacata gcttaatgag gggaactcag ggaactgaaa catctaagta cctgaaggaa
      301 gagaaagaaa ttcgattccg taagtagcgg cgagcgaacg cggattagcc caaaccaata
      361 aagttttctt tattggggtt gcggacatat cataacgaag aggtatcgta attgaagagg
      421 tttggaaaga cccaccacag aaggtaatag tcctgtatgt taaacgagaa gacttcagat
      481 atgatccaga gtaccacggg acacgtgaaa ccctgtggga agcaggaggg accaccctcc
      541 aaggctaaat actacctagt gaccgatagc gtatagtacc gtgagggaaa ggtgaaaaga
      601 accccgggag gggagtgaaa tagaacctga aaccctgcac ttacaagctg tggaagcaca
      661 tttcttgtgt gaccgcgtac tttttgtaga acgggccaac gagttacgtt aagtagcaag
      721 gttaagcact taaggtgcgg agccgtagcg aaagcgagtt ttaactgagc gttcagttac
      781 ttgacgtaga cccgaaaccg ggcgacctac ccatgagcag gatgaagcga aagtaaaatt
      841 tcgtggaggt ccgaacccac gagcgttgaa aagctcgggg atgacttgtg ggtagcggtg
      901 aaattccaat cgagcccgga gatagctggt tctccccgaa atagctttag ggctagcctc
      961 aaggtgagag atacggaggt agagcactga atgtcctagg gggtattgca cctaccgaag
     1021 actatcaaac tccgaatgcc gtcatcttat acttgggagt cagactgtgg gtgataagat
     1081 tcatagtcga aagggcaaca gcccagatcg tcagctaagg tccctaaatg taagttaagt
     1141 ggtaaaggat gtgggattgc acagacaacc aggatgttgg cttagaagca gccactcatt
     1201 caaagagtgc gtaatagctc actggtcgag tgatcctgcg ccgaagattt ccggggctaa
     1261 aacttactac cgaagctacg gcatcagtaa tgatgggtag gggagcttcc catacgggtt
     1321 gaagcatgac cgtaaggaca tgtggacagt atgggagtga gaatgttggc atgagtagcg
     1381 agatgtgggt gagaatccca caggccgtaa acccaaggtt tccaggggaa ggttcgtccg
     1441 ccctgggtta gtcgggacct aagctgaggc cgaaaggcgt aggtgatgga caacaggttg
     1501 atattcctgt actaccgata accgtttgag agaagggatg acacagtagg ataagctaag
     1561 cacactgttg gttatgtgtg cccaagcatt gaggcagtca aagtaggcaa atccgctttg
     1621 ataatgctgg gatgtgatgg ggagcgaaat ttagtagcga agtagctgat ttcacactgt
     1681 caagaaaagt ctctatcgag gttaaaggta cccgtaccgc aaaccgacac aggtgggtga
     1741 ggagagtatc ctaaggccag cgagagaact gttgttaagg aactcggcaa aatgaccccg
     1801 taacttaggg ataaggggtg ccaccatcag gtggccgcag agaataggcc caagcgactg
     1861 tttaccaaaa acataggttt ctgctaagtc gcaagacgat gtataggagc tgacgcctgc
     1921 ccggtgctgg aaggttaagg ggatctgtta gagcaatcga agcagtgaac ttaagcccca
     1981 gtaaacggcg gccgtaacta taacggtcct aaggtagcga aattccttgt cgggtaagtt
     2041 ccgacccgca cgaaaggcgt aacgatttgg gcactgtctc aacaacagac tcggtgaaat
     2101 tgtaattccg gtgaagatgc cggatacctg cgacaggacg gaaagacccc atggagctt
//
LOCUS       DAMJWP010000346         2109 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.353, whole genome shotgun sequence.
ACCESSION   DAMJWP010000346 DAMJWP010000000
VERSION     DAMJWP010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.353"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            73..2097
                     /locus_tag="PC700_003559"
     CDS             73..2097
                     /locus_tag="PC700_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2582820.1"
                     /translation="MDKKKLILITYDKLNSDHYKEELTNFFGDEIIIETQNILDGIKE
                     NLEGEVVLSLSPLTSNFLIKHFKEDIEIIHGTKALSKLGYEKMMKLPPGTKSLLMTTN
                     KTSAFEMATYLYKIGINHIDFVPTYPDCDEIYDLDTAITPGQIRFIPKYIKNIVDLGW
                     RKISLDTYMSLLVVLKLKNEKFIEKLYKLSKETLSHDFLNTSLDNISKLKTILYMTID
                     EIGDGLIFFNTFNKVTFVNKSLLNMLELDEKLIKSPSLMEYMPKSFLDKITKNLNIDN
                     MIIYIDEIDKKFILSKKPFYLYKNIEGCLITLKDVNNIEILEQKIRSDSVKRGYVAKY
                     KFNNIIGNSSIIKDCIKRAKKMALTDNPILITGETGTGKEAFTQSIHNHSNRKNKPFV
                     AINCASLPSELLESELFGYEDGSFTGAKKGGKKGLFELAHTGTIFLDEIGDMPHDLQV
                     KLLRVLQEKEIRKIGGTSIIPIDVRILAATNKDLEKLIIENKFRMDLFYRISMFTLDL
                     PPLRKRLEDIPLLLESFLKELPYKNIKLDKSLLEALNSYTWMGNIRELRNCVEYMAYM
                     GSNYLTINDLPQNISSKLNNNHMSSNMSIFNDLNQYDKNICISILKSLHMKPMGRTKL
                     MKFMEYNVTEYEVRNMLEYLTRNGYLISSKGRKGSSITEKGIKIIENNII"
BASE COUNT          898 a          246 c          301 g          664 t
ORIGIN      
        1 tgttgtataa tataacttac cttatacttt aaatacttta aaaataaata ctattttgtt
       61 aggagaaata aaatggataa aaagaagtta atccttataa catatgataa attaaatagt
      121 gaccactaca aagaagaatt aacaaacttt tttggagacg aaataatcat agaaacacaa
      181 aatatacttg atggaataaa agaaaattta gagggagagg tagtgctttc tctatcacct
      241 ttaactagta attttttaat aaaacatttt aaggaagata ttgaaattat tcatggaacg
      301 aaagcacttt caaaacttgg atatgaaaaa atgatgaaac tacctccagg tacaaaatcg
      361 ttacttatga caaccaataa aacttcagca tttgagatgg ctacttatct atataaaatt
      421 ggtataaatc atatagattt tgtaccgaca tatccagatt gtgatgaaat atatgattta
      481 gatacagcta taacaccagg tcagattaga tttataccaa aatacataaa aaatattgtt
      541 gatttaggtt ggagaaaaat ctcattagat acatatatgt cattattagt agtattaaaa
      601 ctaaaaaatg agaaatttat agaaaaacta tacaaattgt ccaaggagac actaagtcat
      661 gatttcttaa atacgtcatt agataatata tctaaattaa aaactatact ttacatgacc
      721 attgatgaaa taggtgatgg tctaatattt ttcaacacct ttaacaaggt tacttttgta
      781 aataagtctt tacttaatat gttggaacta gatgaaaaac ttataaaatc accttcctta
      841 atggaatata tgccaaagag tttcttagat aaaattacaa agaacttaaa tatcgataat
      901 atgattatat acatagacga aatagataaa aaattcatac tttctaaaaa accattttat
      961 ctttataaaa atatagaggg ttgcttaatt acattaaaag atgtaaacaa tatagaaata
     1021 ttagaacaaa aaataagaag tgattctgtc aaaagaggtt atgtagctaa atataaattt
     1081 aataatatta ttggaaatag ttctattata aaagattgta ttaaaagggc aaaaaaaatg
     1141 gctcttacag ataatcctat attaataaca ggtgaaacag gaacaggtaa agaagccttt
     1201 actcagtcaa tacacaatca ttcaaataga aaaaataaac cttttgtagc aataaattgt
     1261 gcttcattac caagtgaatt acttgaaagt gaactatttg gatatgaaga tggttctttt
     1321 actggagcaa aaaaaggtgg aaaaaaagga ttattcgaat tagctcatac tggcactata
     1381 tttttagatg agataggaga tatgccacat gatttacagg ttaaactttt aagagtctta
     1441 caagaaaaag aaattagaaa aattggtggt acaagtatta ttccaattga tgtgagaata
     1501 ttagctgcca caaataaaga tttagaaaaa ttaattatag aaaataaatt taggatggat
     1561 ttattttata ggataagtat gttcacttta gatttacctc ctcttagaaa gagattagaa
     1621 gatatacctc ttcttttaga atcattttta aaagaacttc cctataaaaa cataaaactc
     1681 gataagagtc ttttagaagc tttaaattca tacacttgga tggggaatat tagagaactg
     1741 agaaattgtg ttgaatatat ggcttacatg ggttccaatt atcttacaat aaatgattta
     1801 cctcaaaaca tatcttcaaa attaaataat aatcatatgt caagtaatat gtctatattc
     1861 aatgatttaa accaatatga caaaaacata tgcatttcta tcctaaaatc attgcacatg
     1921 aaaccaatgg ggaggacaaa gttaatgaaa tttatggaat ataatgtcac agaatacgaa
     1981 gtaagaaata tgctagaata tttgacacgc aatggatact taatttctag taaaggaaga
     2041 aaaggctcat caataactga gaaaggcata aaaataatag aaaataatat aatttaaacc
     2101 tatagtaat
//
LOCUS       DAMJWP010000347         2105 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.354, whole genome shotgun sequence.
ACCESSION   DAMJWP010000347 DAMJWP010000000
VERSION     DAMJWP010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.354"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            345..1205
                     /locus_tag="PC700_003560"
     CDS             345..1205
                     /locus_tag="PC700_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2582821.1"
                     /translation="MKKQKSPILLEMLGLFLGCISMSIGINMFLKPHTIAPGGLSGLS
                     LVLNKVTGLPVSAIMLIIGVPLVILAFRIMGTKNSLKTLFGTVVFSAIVQLTDPLSKL
                     RFTNDLLLSSIAGGILVGIGLGIMFKSDASTGGTDLIALILSKKFPGIKATKFMSCLD
                     GMVVISSGIVNRSLETALYSGIALCVLIKIADMIMEGFDHSKAFFIISDEPESLRQAI
                     TEELDRGLTILDGKGGYTRENKEVLLVVVSKKQELYLKRLVKRTDPTAFVIVTDVHEV
                     LGEGFKNLEN"
     gene            complement(1445..2002)
                     /locus_tag="PC700_003561"
     CDS             complement(1445..2002)
                     /locus_tag="PC700_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2582822.1"
                     /translation="MSDSEYPQMKTLETRSCILRPASLDDVEDFFDCYKNKIVVKHLP
                     FSEHKSLEDTRKFIKSFFLNPYKKGKIGHFAIVYKRDNKVIGNMGFNNINPKALEAEI
                     GICINPSYWGHDFATEITKRVIQYGFRELNLNKIIAITYEENQNSTKSLDLLGFKCTG
                     KYIKKIHTGNTVKNVPCYQYELKKQ"
BASE COUNT          765 a          273 c          276 g          791 t
ORIGIN      
        1 ttaataaatc cttttgataa catataaaag caagataatt ttttccatta aataccttat
       61 aattatacac aaaatctcta tatacgttaa acagtataga gaaaattttc atagaaccat
      121 ctttataggg gatactttat aaaagaataa aaaaattata atatataaaa aacttcatag
      181 gagtgtaaca acatcagtaa agatatgaaa acactatata aaaattcaaa aatattaaga
      241 ataatgaaat acttgaaatt gcttatatca agaaatttta tataatataa actggtcaaa
      301 tatgttataa tattaaattg tgacaagttc atggaggtgt ttatatgaaa aaacaaaagt
      361 cgccaatttt acttgaaatg cttggactat ttttgggttg tatatcaatg agtattggta
      421 taaatatgtt tttaaaacca catacaattg ctcctggagg acttagtggt ctatcattag
      481 tactaaacaa ggttacagga cttccagtat cagctatcat gttaattata ggtgtcccat
      541 tagtaatact tgcttttaga attatgggta caaaaaattc attaaaaact ttatttggaa
      601 cagttgtatt ttcagctata gttcaattaa cagacccatt atcaaaacta agatttacta
      661 atgacttatt attgtcttct attgcaggag gaatcctagt tggtataggt ttaggaataa
      721 tgtttaaatc agatgcttct actggaggaa cagacttaat tgcactcata cttagtaaga
      781 aattcccagg aataaaagct acaaaattca tgtcatgcct agatggaatg gttgttatat
      841 cttctggtat agtaaatcgt agtttagaaa ctgctcttta ctctggtata gcactttgtg
      901 tattaataaa aatagcagat atgataatgg aaggatttga ccattcaaaa gcatttttta
      961 ttatctctga tgagcctgaa agtttaagac aagctataac tgaagaactt gatagaggtc
     1021 ttactattct tgatggtaaa ggtggataca ctagagaaaa taaagaagtt ctattagtcg
     1081 tagtttctaa aaaacaagaa ctttatctaa agagattagt taaaagaact gaccctactg
     1141 catttgttat tgttacagat gtgcatgagg ttcttggaga aggatttaaa aatttagaga
     1201 actaataaaa attaatattt taagtatata tactatttta attaaaatac gatttattca
     1261 aaaagagtgt ctcataaact ttttagttca tgagacactc ttttttgtat aaaatcaaat
     1321 gcattttcag tattttaaca ataaaaaaca ggattatcta tattttgagt caactacttt
     1381 attgaaattt tatttagaaa acaatcatta ctaaataacc aggatagata ttaaatgatt
     1441 atatctattg ttttttcaat tcatattgat aacaaggaac attttttaca gtatttccag
     1501 tatgaatttt tttgatatac tttcctgtac atttaaatcc taataaatct aatgattttg
     1561 tagaattttg gttttcttca taggttattg caattatttt atttaagttt agttctctaa
     1621 atccatactg tattactctt tttgttatct cagtagcaaa atcatgtccc caatagcttg
     1681 gatttataca tatacctatt tctgcttcta atgctttagg atttatatta ttaaacccca
     1741 tattcccaat aactttattg tctcttttat atactatagc aaagtggcca attttccctt
     1801 ttttataggg atttagaaaa aatgatttta taaactttct tgtatcttct aaagatttat
     1861 gctcactaaa tggtaaatgt tttactacta ttttattttt ataacagtca aaaaaatctt
     1921 caacatcatc taatgatgca ggtcttagta tacaacttct agtttctaag gttttcattt
     1981 gaggatattc agaatcagac atttctaatc cccctttata cattaatcta aatatactgt
     2041 tcatatttaa aatacgatat taattatata aatgttctca aattaacctt atctttattt
     2101 ttaat
//
LOCUS       DAMJWP010000348         2036 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.355, whole genome shotgun sequence.
ACCESSION   DAMJWP010000348 DAMJWP010000000
VERSION     DAMJWP010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.355"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            194..460
                     /gene="rpsT"
                     /locus_tag="PC700_003562"
     CDS             194..460
                     /gene="rpsT"
                     /locus_tag="PC700_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDF2582823.1"
                     /translation="MANIKSAKKRISVIEKKTALNRVRKSQIKTAIRRFEDAVAAGNR
                     EDAVAKFQYAQKRIYQVASKGTIHKNAAARKVAKLAQKLNGMNA"
     gene            complement(647..1690)
                     /gene="holA"
                     /locus_tag="PC700_003563"
     CDS             complement(647..1690)
                     /gene="holA"
                     /locus_tag="PC700_003563"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDF2582824.1"
                     /translation="MNYKDIIRNVKEKNFEKMYLFYGREYYLIENAIKAFKDSLNEGM
                     LDFNLDIIDGKEIVLNQLISSIETLPFMDDRKIVIIKDFELLKGKKKNFTDSDEKYLI
                     EHLDNIPDTTTIVFVVYGDVDKRKSLVKKIGNNGIVFDCDKLSDMDLFKWIKKSFALN
                     DVIIDNSQIMYFIEQEGYRDKSSEKTLSDLENEINKISSFVGKGNNVTNDVIDKLSQK
                     KVENDIFKLIDYIGEQNASNAMKILNDMIQEGESVLGIFSMIARQFKIIMQVRQLQLD
                     GYSTKLIADKLKMHQFVVGKALKQTKNFSDDIIVEILNYILESDYKIKTGLIRDTLAV
                     EMLVSRYCKREAI"
     gene            complement(1644..>2036)
                     /locus_tag="PC700_003564"
     CDS             complement(1644..>2036)
                     /locus_tag="PC700_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HDF2582825.1"
                     /translation="SSNILTLPIIYYTFKGIPLLSIIGNLIIVPFVGVIMYLSIASLI
                     VFKVSVVIAKIISFFNSTLIESIFFLLEKISNLSFAYINIESPKFYIVVIYYIGVFFY
                     IFYIEGKEIKEQENELQGYYKECKREKL"
BASE COUNT          746 a          300 c          197 g          793 t
ORIGIN      
        1 ttaaaacaca aaatatacca atttaaaaac aagatattgc aaattattta tacacaattt
       61 acagtctaaa aaatcaaaat aaaaaacatt attaaaatat ataaataata atccttgatt
      121 tttagattta tttctgttaa aatctaatat gttgacgatg aatatgataa aaaaatacag
      181 ggaggtgaac acggtggcaa atataaaatc agctaagaaa agaataagtg ttatcgaaaa
      241 gaaaactgct ttaaatagag ttagaaaatc tcaaataaaa actgcaataa gaagattcga
      301 agatgctgtt gcagctggaa acagagagga tgctgttgca aagttccaat acgctcaaaa
      361 aagaatatac caagtagctt ctaaaggaac tatacataag aatgctgcag ctagaaaagt
      421 agctaaatta gctcaaaaat taaacggtat gaacgcttaa ttaccagttt atatttctta
      481 tatataataa cccctatgta ggggtttttt attttgtaaa tttatacaat aaaaaataaa
      541 tagttagaca tattatagtt gattgtatat caaacttaat ttagcttaca tctactctga
      601 actataacat atcttgctat ttatttttta ttaatatata gattttttaa attgcttctc
      661 ttttacagta tctactaacc aacatttcaa ctgctaaagt atctcttata agacctgttt
      721 ttattttata atcactttct aatatatagt ttaatatttc cacaattata tcatcagaaa
      781 aatttttagt ttgctttaat gcttttccta caacaaattg atgcattttt aatttatcag
      841 ctataagttt tgttgaatat ccatcaagtt gtaactgtct tacttgcata attattttaa
      901 attgtcttgc tatcatagaa aatataccta atacagattc gccttcttgt atcatatcat
      961 ttaatatttt cattgcatta gaagcatttt gttctccaat ataatcgatt aatttaaata
     1021 tatcattctc aacttttttt tgagataatt tatcaattac gtcattggtt acattattcc
     1081 ctttaccaac aaaggaactt attttattta tttcattttc taaatcagat aatgtctttt
     1141 cactgctctt atctctgtat ccttcttgtt caataaaata catgatttga gaattatcta
     1201 ttataacatc atttaaagca aaagattttt ttatccattt aaataaatcc atatcagaaa
     1261 gcttatcaca atcaaaaaca attccattgt ttcctatttt cttaactaat gattttcttt
     1321 tgtctacatc tccatataca acaaaaacta tagttgtagt gtctggaata ttatctaaat
     1381 gctctattaa gtatttctca tcactatctg taaaattctt tttctttcct tttaatagtt
     1441 cgaagtcttt tattattact atctttctat catccataaa tggaagtgtt tctatagagc
     1501 ttataagctg atttaagact atttccttcc catctattat atctaaatta aaatcaagca
     1561 ttccttcgtt tagactatct ttaaatgctt ttattgcatt ctcaattaaa taatactctc
     1621 ttccataaaa aagatacatt ttttcaaagt ttttctcttt tacattcctt ataatatcct
     1681 tgtaattcat tctcttgctc ctttatctct tttccttcta tgtaaaatat gtaaaaaaac
     1741 actcctatgt aataaataac aacaatataa aatttaggac tttctatatt aatgtatgca
     1801 aaacttaaat tgctgatttt ttctagtaaa aaaaatatgc tttctatcaa tgtactatta
     1861 aaaaaagata taattttagc tattactaca cttactttaa atacgattaa acttgcaata
     1921 cttaaataca ttattactcc cacaaatggt actataatta aatttcctat aattgaaagc
     1981 aatggtatac ctttaaatgt ataataaatt ataggtaaag ttaatatatt tgatga
//
LOCUS       DAMJWP010000349         2031 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.356, whole genome shotgun sequence.
ACCESSION   DAMJWP010000349 DAMJWP010000000
VERSION     DAMJWP010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.356"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            154..768
                     /locus_tag="PC700_003565"
     CDS             154..768
                     /locus_tag="PC700_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDF2582826.1"
                     /translation="MEEVIAKIITIENGFKEIENIAKKIVKNNDSKKCYYLSIYLYRS
                     EYYQVRELAVFILGFISVTISEALLFLKDNVSKDENWRVQEILAKSFDYYCSEVGYEK
                     ALPVIKEWLNSDSPNIKRAVTEGLRIWTSRDYFKSNPDVAISLLSSLKEDDSEYLRKS
                     VGNALRDISKKYPDLVKKELDSWDISNKKVEFVYKLASKCILAK"
     gene            786..1148
                     /locus_tag="PC700_003566"
     CDS             786..1148
                     /locus_tag="PC700_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2582827.1"
                     /translation="MKLRDEYTCPLELTHDMIKGKWKSIILWRLRIGPTSLSKLEKEI
                     NGINQKMLLEQLKELIEFGLVDKKTYKGYPLKVEYSLTPIRGKQILDALAIMQDIGIS
                     FMLDNGMEDILKEKGVLK"
     gene            1254..1751
                     /locus_tag="PC700_003567"
     CDS             1254..1751
                     /locus_tag="PC700_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HDF2582828.1"
                     /translation="MKVTSTGIVNGVIEDKYGKRGTQFNEGGMPTYSLPLKFEDAPKN
                     TVSFAVFLEDKDSVPVCGFAWIHWLAVNITKDELNENESVTATDFVQGTTSWHGKLGG
                     LDRLEASVYGGMAPPDRPHVYEIHVFALDTILDLEKGFYMNEFFKAIEGHVLDTFTLK
                     GSYSN"
BASE COUNT          771 a          228 c          334 g          698 t
ORIGIN      
        1 aatgaggttg attttttatt attttttatt tatgctagta ctaaagactt gcaaacgttt
       61 tttgacaagt ctttattttg tctaaattaa acaaactttt ttacaaataa atacataaaa
      121 tttaaactaa tctatttatt ggaggagata aaattggaag aagtaattgc aaaaattatt
      181 actatagaaa atggattcaa agaaattgaa aacattgcta aaaaaattgt aaaaaataat
      241 gactcaaaaa agtgttatta tctatcaatc tacttatata ggtcagaata ttatcaagta
      301 agagaattgg ctgtgtttat acttggtttt atatcagtaa ctatttctga agctttgtta
      361 tttttaaaag acaatgtgag taaagatgaa aactggagag tacaagaaat actagctaaa
      421 tcatttgatt attattgcag tgaagtagga tatgaaaagg cacttcctgt tataaaagaa
      481 tggcttaatt ctgatagtcc aaacataaaa agggctgtga cagaaggtct tagaatttgg
      541 actagtagag actattttaa atctaatcct gacgttgcta tatcactact ttctagttta
      601 aaagaagatg atagcgaata cttgagaaag tctgtaggta atgctcttag agatataagt
      661 aaaaaatatc cagatttagt aaaaaaagaa ttagattcat gggatatatc aaataaaaaa
      721 gttgagtttg tatataagct tgcttcaaaa tgtatcttgg caaagtaaaa tggaggtgaa
      781 cttatttgaa acttcgtgat gagtatactt gcccattaga actaacacat gatatgataa
      841 aaggcaaatg gaaatctatt atattatgga gattacgcat aggaccaacc tccttatcaa
      901 aacttgaaaa ggaaatcaat ggtattaatc aaaagatgtt acttgaacaa cttaaagaat
      961 taattgaatt tggtcttgta gataaaaaga catataaagg ttacccactt aaagttgaat
     1021 actcacttac acctattcga gggaaacaaa tattggatgc tttggctata atgcaagata
     1081 taggaattag ctttatgttg gataatggta tggaagatat tcttaaggaa aaaggtgttt
     1141 taaaataaat agatacccac aaaaaagtaa gtaatttacc attattttca actggtatat
     1201 aattaaatta tagatatatt ctattaattt attataatag gaggtaaatt attatgaaag
     1261 taactagtac aggtatagta aatggtgtta tagaagataa atacggtaaa agaggaactc
     1321 agttcaatga aggtggtatg cctacatatt cactacctct aaagtttgaa gatgctccaa
     1381 agaatacagt atcctttgct gtatttttag aagataaaga ctctgttcct gtatgtgggt
     1441 ttgcatggat acattggtta gctgtaaata taacaaaaga tgagttaaat gaaaatgaaa
     1501 gtgtaacagc tactgatttt gtacaaggga ctactagttg gcatggcaaa ctaggaggtc
     1561 ttgacagatt agaggccagt gtatatggtg gaatggctcc tccagacaga cctcatgtct
     1621 atgaaataca tgtatttgca cttgatacta tccttgacct tgaaaaagga ttctatatga
     1681 atgaattttt caaagctatt gaagggcatg ttttagatac atttacacta aaaggaagct
     1741 attctaatta attaggttta ctatagctaa tcattaatta aatgctacat ttaattgatg
     1801 attagcttaa ttgttattag aatattttct gcactttatc ttaatatata ttttagttga
     1861 ctggtatttt aatttctatt agattattat caatttatat aaattgattt tagcttaaaa
     1921 cttacttatc tatttcagtt tctattagaa ttattattag actcattaaa ataaaagcat
     1981 taatataact aataatatat gttaaattca ttaactctac ataaagtttt a
//
LOCUS       DAMJWP010000350         1993 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.357, whole genome shotgun sequence.
ACCESSION   DAMJWP010000350 DAMJWP010000000
VERSION     DAMJWP010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.357"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            107..676
                     /locus_tag="PC700_003568"
     CDS             107..676
                     /locus_tag="PC700_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582829.1"
                     /translation="MKLRNPKIKKLLSVALLAALIGIPIGQTANAMEISNNVPSYIEN
                     NPTTIEDLEINDSQLPKPGTVTYIDINKLIKNLKENGIDTTLIENELYYRKAGTNKIV
                     WTKTGFDLYLSKGVALTLAGAGTGVAGLLAGAIPGIGQYLAAAIVGAITMHLGSNITS
                     GKIFKFKKMRYNKGGAYVTKYYLQSVRNQ"
     gene            968..1285
                     /locus_tag="PC700_003569"
     CDS             968..1285
                     /locus_tag="PC700_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582830.1"
                     /translation="MDFGNINLILIGIIVIIGTTIIYLIKPKTAFCSKKYFNKLESIY
                     GNIDKKKTVKLEVLYRYVTGLEYISIGLFTRRLDITIIAIILVATITVILYYLVRKRY
                     ITI"
BASE COUNT          813 a          226 c          269 g          685 t
ORIGIN      
        1 tactatattt tttattaata tttaatgtag ttaattctta agaattatat tctacaataa
       61 taatatatat ctttgatata taactaattt aaaatggagg tattttatga aactaagaaa
      121 tccaaagatt aagaaattgt tatcagtagc attattagca gctcttatag gtatacctat
      181 tggacaaaca gccaatgcaa tggaaatttc aaataacgtc ccaagttata tagaaaacaa
      241 tccaacaaca atagaagact tggaaatcaa tgactctcaa ttacctaaac caggtacagt
      301 tacatacata gatattaata agctaattaa aaaccttaag gaaaatggta tcgacactac
      361 tcttattgaa aatgaattat attatagaaa agctggaaca aataaaatag tttggactaa
      421 gacaggcttt gatttatatc tttctaaagg agttgcatta actttagctg gtgctggtac
      481 aggggttgca gggcttttgg ctggtgccat tcctggtata ggacagtatc ttgcagctgc
      541 tatagttgga gcaattacaa tgcatcttgg ttcaaatata acatctggaa aaatctttaa
      601 atttaaaaaa atgcgttata ataaaggtgg ggcttatgta accaaatatt acttacaatc
      661 tgtaagaaat caataggtga taaaatgatt ataaaatatc tactttttgc aattatctca
      721 gcactagttt tattcttttg taataaagta tttcaactaa gtaaattaca caagttcatt
      781 atattgtttg tttcattttt tatactaact tttgtagcat catttattaa tagataaaaa
      841 ataagatggt ggggttaacc taccatcttt ttctcattta atatttgtaa tattttagat
      901 actctttata cttgtgttac tgatatactt tgattaagaa aagtaaattt aacaggaggt
      961 atctgctatg gattttggaa atatcaatct tatacttatt ggaataatag ttataatagg
     1021 aactacaata atatatttaa ttaaacctaa aactgctttt tgtagtaaaa aatattttaa
     1081 taaattagaa tcaatatatg gaaatataga taagaaaaaa actgttaaac tagaagtact
     1141 ttatcgctat gtaacaggtc ttgagtatat atcaataggc ttatttacaa gaagattaga
     1201 tattacaata atagcaataa tattagtagc tactataaca gtaatattat attacttagt
     1261 tagaaagaga tatataacta tataaaatct aatagaatta atatcaaaag aaagtagcaa
     1321 ctaatcacca gtaatgtaaa ataatttgtt tttcaaagtt aataaaagtc ttctttatgg
     1381 caatgattta aatataatca aatatcgtaa cggagatttt tttatgaata atttaagaaa
     1441 acaattaatc aaagaaatta tataagatgg agaacttata aaatctacag atataagtag
     1501 ttttaggagt aaatacagat gacttatttc aaagagacaa tagctacaag ttatcctcat
     1561 tctgtaatac aaaaatgttt aatacattag ataagaaata ctttaaatat gtttcataca
     1621 aggactcaaa agaattaata agtgatttta aatctgtata taggtcagtt aatgaataaa
     1681 aagccttatc gactttagat aaaatagatt aaaaataatg taaacagtat acttcagaaa
     1741 ttaaaacgtt tggaaataac tggaatgttg tagcatcagc ctttagtttt actaaaataa
     1801 gtaatatcta taatatatat tacaagtata atcgaaggat ttaataaata tttcaaaaat
     1861 gtctcaaaaa caaagtccag atttacatca aacgcttcac tcttaaagat attattcttg
     1921 acaagcgaaa atgtgatgaa aaaatggact atgaaatata aaaattgata taatatatac
     1981 aatcatttat tca
//
LOCUS       DAMJWP010000351         1983 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.358, whole genome shotgun sequence.
ACCESSION   DAMJWP010000351 DAMJWP010000000
VERSION     DAMJWP010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.358"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            178..294
                     /gene="rrf"
                     /locus_tag="PC700_003570"
     rRNA            178..294
                     /gene="rrf"
                     /locus_tag="PC700_003570"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            301..375
                     /locus_tag="PC700_003571"
     tRNA            301..375
                     /locus_tag="PC700_003571"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:333..335,aa:Asn,seq:gtt)
     gene            380..454
                     /locus_tag="PC700_003572"
     tRNA            380..454
                     /locus_tag="PC700_003572"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:413..415,aa:Glu,seq:ttc)
     gene            460..535
                     /locus_tag="PC700_003573"
     tRNA            460..535
                     /locus_tag="PC700_003573"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:493..495,aa:Val,seq:tac)
     gene            541..617
                     /locus_tag="PC700_003574"
     tRNA            541..617
                     /locus_tag="PC700_003574"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:575..577,aa:Asp,seq:gtc)
     gene            628..702
                     /locus_tag="PC700_003575"
     tRNA            628..702
                     /locus_tag="PC700_003575"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:660..662,aa:Thr,seq:tgt)
     gene            717..801
                     /locus_tag="PC700_003576"
     tRNA            717..801
                     /locus_tag="PC700_003576"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:751..753,aa:Tyr,seq:gta)
     gene            811..884
                     /locus_tag="PC700_003577"
     tRNA            811..884
                     /locus_tag="PC700_003577"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:843..845,aa:Gly,seq:tcc)
     gene            895..971
                     /locus_tag="PC700_003578"
     tRNA            895..971
                     /locus_tag="PC700_003578"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:929..931,aa:Arg,seq:tct)
     gene            981..1056
                     /locus_tag="PC700_003579"
     tRNA            981..1056
                     /locus_tag="PC700_003579"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1014..1016,aa:Gln,seq:ttg)
     gene            1068..1143
                     /locus_tag="PC700_003580"
     tRNA            1068..1143
                     /locus_tag="PC700_003580"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1101..1103,aa:Lys,seq:ttt)
     gene            1146..1234
                     /locus_tag="PC700_003581"
     tRNA            1146..1234
                     /locus_tag="PC700_003581"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1180..1182,aa:Ser,seq:tga)
     gene            1252..1342
                     /locus_tag="PC700_003582"
     tRNA            1252..1342
                     /locus_tag="PC700_003582"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1286..1288,aa:Ser,seq:gct)
     gene            1351..1427
                     /locus_tag="PC700_003583"
     tRNA            1351..1427
                     /locus_tag="PC700_003583"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1385..1387,aa:Pro,seq:tgg)
     gene            1513..1588
                     /locus_tag="PC700_003584"
     tRNA            1513..1588
                     /locus_tag="PC700_003584"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1546..1548,aa:Trp,seq:cca)
     gene            1649..1725
                     /locus_tag="PC700_003585"
     tRNA            1649..1725
                     /locus_tag="PC700_003585"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1683..1685,aa:Pro,seq:tgg)
     gene            1732..1808
                     /locus_tag="PC700_003586"
     tRNA            1732..1808
                     /locus_tag="PC700_003586"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1766..1768,aa:Ile,seq:gat)
     gene            1812..1887
                     /locus_tag="PC700_003587"
     tRNA            1812..1887
                     /locus_tag="PC700_003587"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1845..1847,aa:Phe,seq:gaa)
     gene            1895..1971
                     /locus_tag="PC700_003588"
     tRNA            1895..1971
                     /locus_tag="PC700_003588"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1929..1931,aa:Met,seq:cat)
BASE COUNT          501 a          449 c          535 g          498 t
ORIGIN      
        1 aatgtattta gcttatcgat actaataggt cgaggacttg accaatatta tttgatgtta
       61 ttcattaaga tatatgtgca gtttttagag tgttaactct aaaaaaaaat aaaaaaagta
      121 ttgacaaaaa tagtaaaaaa tgttaatatt atacttgtcc tagcaacaag aaaaaaatgt
      181 ggttataata gcagagaaga tacacctgtt tccattccga acacagaagt taagttctct
      241 agcgctgatg gtacttggtg ggaaactgcc tgggagagta gggcatagcc acgtgaatgt
      301 tccaaggtag ctcaacggtg gagcaaccgg ctgttaaccg gtaggctgtg ggttcgagcc
      361 ccacccttgg agccattatg gctcgttggt caagaggtta agacaccgcc ctttcacggc
      421 ggtaacaggg gttcaattcc cctacgagtc accatttgtg ggatcatagc tcagctggga
      481 gagcacctgc cttacaagca gggggtcaca ggttcgagcc ctgttggtcc caccatttgc
      541 ggcctggtag ttcagttggt tagaatgcca gcctgtcacg ctggaggtcg agggttcgag
      601 ccccttccag gtcgccaaaa taaaaatgct ggtgtggctc aacggtagag cagctgactt
      661 gtaatcagca ggttgtaggt tcgattccta tcaccagctc catgtaaaac taatacggag
      721 gatttcccga gcggccaaag ggggcagact gtaaatctgt tgtcatcgac ttcggtggtt
      781 cgaatccacc atcctccacc aattaaatat gcgggtgtag ctcaatggta gagttctggc
      841 cttccaagcc agctgtgagg gttcgatccc cttcacccgc tccaaagaaa gcgtgggtgc
      901 atagctcagc tggatagagc aacgcccttc taaggcgtgt gtccggggtt cgaatccctg
      961 tgcgctcacc acatatatat tggggattag ccaagtcggt aaggcacatg actttgactc
     1021 atgtatgcgt aggttcgagt cctgcatccc cagccaaata aaaatatggt ctattagctc
     1081 agtcggtaga gcacctgact tttaatcagg gtgtcccgcg ttcgagtcgc ggatagatca
     1141 ccaatggaga ggtgtccgag tggtttaagg agctggtctt gaaaaccagt gacttcgaaa
     1201 ggggccgtgg gttcgaatcc caccctctcc gccaaaaaaa tgactgaatt tggagaagta
     1261 ctcaagtggc tcaagaggat cccctgctaa gggattaggc tgggtaaccg gtgcgagggt
     1321 tcgaatccct ccttctccgc cattcaaaaa cgaggtgtag cgcagtttgg tagcgcacat
     1381 ggtttgggac catggggccg ggggttcgag tcccttcacc tcgaccaact taagctttta
     1441 gttcagttgg ttagagtgcc caactcatgg ctagtaattc tagggtttga gtccatgaag
     1501 gttattcttc ataggggtgt agctcagttg gtagagcgtc ggtctccaaa accgtgcgcc
     1561 gggggttcga gtccctccac ccctgccaaa taaaaaggac ttgaaaaaag ttggatttta
     1621 gagtagtata agatagtaaa taagatttcg aggtgtagcg cagtttggta gcgcacatgg
     1681 tttgggacca tggggccggg ggttcgagtc ccttcacctc gaccaagaaa agggcctata
     1741 gctcaggtgg ttagagcgca cgcctgataa gcgtgaggtc gctggttcga gtccagctag
     1801 gcccaccatt tgcccagata gctcagtcgg tagagcaggg gactgaaaat ccccgtgtcg
     1861 gtggttcgat tccgcctctg ggcaccagga atacggcggt atagcttagt tggctagagc
     1921 gttcggttca tacccgaaag gtcataggtt cgactcctat taccgctacc atctaactat
     1981 atc
//
LOCUS       DAMJWP010000352         1898 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.359, whole genome shotgun sequence.
ACCESSION   DAMJWP010000352 DAMJWP010000000
VERSION     DAMJWP010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.359"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            40..414
                     /locus_tag="PC700_003589"
     CDS             40..414
                     /locus_tag="PC700_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2582831.1"
                     /translation="MNIVISNSSGKPIYEQITTQIKSMIISGELPEGSALPSMRLLAK
                     ELRISVITTKRAYSDLERDGFIETVTGKGSFVSSKNMDFVKEEQFRLIEEYLQKAVDT
                     AKSSDITYDELKDILFLLYKGE"
     gene            411..1274
                     /locus_tag="PC700_003590"
     CDS             411..1274
                     /locus_tag="PC700_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2582832.1"
                     /translation="MISMDAITIKNLNKTYKDFSLQDISFSVPKGSVMGFVGENGAGK
                     TTTLKAILNLISYDSGNIEIFGLDSKKNEKEIKEQIGVVFEGSNFHENLNTDHVSKIM
                     SKIYKNWNDTLFKDYLKKLRVPDNKLIKEFSKGNKMKLSIAVALSHKPKLLILDEATS
                     SLDPIVREEILDIFLDFIQDEEHSIILSSHITSDLDKIADYITFIHKGKIVFSENKDE
                     LIDTMGVLKCKPSDFDNLSREDYSYYRKSQFGYEVLLKDKHRFISRNPNCIVDNTSIE
                     EIMLFYVRGDK"
     gene            1274..>1898
                     /locus_tag="PC700_003591"
     CDS             1274..>1898
                     /locus_tag="PC700_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HDF2582833.1"
                     /translation="MKGLILKDLLNLKGNVKFILLFIIMFGFMSSLGDGNVNNFIGVI
                     IVLCTTMIVSTFSYDDLNKWDSYVLTMPINRNDIVLSKYLTMLIFSFIGVLVSLIVSV
                     TIGYFKNTLILNETLLINALILSISVCFGSLILPLIYKFGTERARLLMILCFLVPTLA
                     LLVFKSILENISSPISIEIILNTLVYSLPFVAILLFVISYFISSKIYS"
BASE COUNT          724 a          197 c          297 g          680 t
ORIGIN      
        1 tgtgtatata ctatatatac aatttaagga ggtgacttat tgaatatagt tattagtaat
       61 tcgagtggga agccaatata tgaacagatt actacacaaa ttaaaagtat gattataagc
      121 ggagagttac cagaaggctc agctcttcca agtatgagac tattggcaaa ggaacttaga
      181 ataagtgtaa ttactacaaa acgagcatat agcgatttag aacgagacgg atttattgag
      241 acagttactg gtaaaggcag ttttgtatct agtaaaaaca tggattttgt caaagaagaa
      301 caatttcgtt taattgaaga atacctacaa aaagcagttg atacagcaaa aagtagtgat
      361 attacatatg acgaattaaa agatatttta tttttattat ataaaggaga atgataagta
      421 tggatgcaat aactataaaa aatttaaata aaacttataa agattttagc ctacaagata
      481 tatctttttc agtaccaaaa gggagtgtaa tgggatttgt aggtgaaaat ggtgcaggaa
      541 aaacaactac attaaaagca attttaaatt taatatctta tgatagtgga aatatagaaa
      601 tatttggtct tgatagtaaa aaaaatgaaa aagaaattaa agagcaaatt ggggttgtat
      661 ttgagggaag caactttcat gaaaatttaa acactgacca tgtttcaaaa atcatgagta
      721 aaatctataa aaattggaat gacacattat ttaaagacta cttaaaaaaa ttgagagtac
      781 cagacaataa attaattaag gagttttcaa aggggaataa gatgaaactt agtattgctg
      841 tagccttatc tcataaacca aaattattaa tactagatga ggcaactagt tcacttgacc
      901 ctatcgttcg tgaagaaata ctagatatat ttttagattt tatacaagat gaagaacact
      961 ctattatctt atcttcacat attacaagtg atttagataa gatagcagac tatattacat
     1021 ttatacataa aggaaaaatt gtatttagtg aaaataagga tgaattaatt gatactatgg
     1081 gagttttaaa atgtaaacct agtgattttg ataacctttc aagagaggat tattcatatt
     1141 atcgtaaaag ccaatttgga tatgaagtac ttcttaaaga taaacataga tttatatcaa
     1201 gaaatccaaa ttgtattgtt gataatacgt ctatagaaga aattatgtta ttttatgtga
     1261 gaggtgataa ataatgaaag gtttaatttt aaaagattta cttaatttga aaggaaatgt
     1321 aaagtttata cttttattca tcattatgtt tgggtttatg tcttctttag gagatggaaa
     1381 tgttaataac tttataggtg ttattatagt tttatgtact acaatgattg tttcaacatt
     1441 ttcatatgat gatttaaata aatgggatag ttatgtactt acaatgccta ttaatcgcaa
     1501 tgatattgta ttaagtaaat atttaactat gttaatattt agctttatag gtgttcttgt
     1561 ttcacttatt gttagtgtaa ctattggtta ttttaaaaat acacttatat taaatgaaac
     1621 actacttatt aatgcactaa tcttatcaat ttcagtatgt tttggtagcc ttattctacc
     1681 acttatttat aaatttggta cagaaagagc tcgtttatta atgattttat gctttttagt
     1741 tccaacacta gctttattag tttttaaaag tattttagaa aatattagtt cacctatttc
     1801 tatagaaatt atacttaata cactagtata ttctcttcca tttgttgcta ttttactatt
     1861 tgtgatttca tattttatat catcaaaaat ctatagta
//
LOCUS       DAMJWP010000353         1866 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.360, whole genome shotgun sequence.
ACCESSION   DAMJWP010000353 DAMJWP010000000
VERSION     DAMJWP010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.360"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(118..333)
                     /locus_tag="PC700_003592"
     CDS             complement(118..333)
                     /locus_tag="PC700_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582834.1"
                     /translation="MHINQTGFISNGLGIKQQQKKEPTSNSFSYNAPSSWKPVGRDSV
                     QYNCGFELIATLVREGSSWAFNFIVQP"
     gene            complement(516..860)
                     /locus_tag="PC700_003593"
     CDS             complement(516..860)
                     /locus_tag="PC700_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582835.1"
                     /translation="MNYRLKKSVTTILSLLIMTILLPTNASASKVEDSSMKVMYSEKQ
                     VTDMDKLYDMAKNGITDVKSSNEKGIIKNARTGESIYVDSISTTQLLEVKQSKNLTSK
                     IYSKTVFLILIQ"
     gene            987..1370
                     /locus_tag="PC700_003594"
     CDS             987..1370
                     /locus_tag="PC700_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582836.1"
                     /translation="MRKKFFIGILIVLIISVIAILNIKNDKNVFIGESENWKAKFVTT
                     EGHSKLEIKYKNSKDVVEQKFNYSYQFIDGEDAVVVVKNEILEAGQEKAIKIQRPGLY
                     SNRESSYKLIISWNGNEETINLKKK"
BASE COUNT          698 a          234 c          232 g          702 t
ORIGIN      
        1 aataagcaat tgttactata acgtaggaat tatctttaaa aacacaaatt aatttacatt
       61 ctctaatttt aaagtatatg tatatctttg attttaataa aattcactta aaatatttta
      121 aggttgaaca atgaaattga atgcccaaga acttccttct cttactaatg ttgcaattaa
      181 ttcaaaccca caattgtatt gaactgaatc tctacctact ggtttccaac tagaaggtgc
      241 attatatgag aaagaatttg atgttggctc tttcttttgt tgctgcttaa ttcctagccc
      301 attagaaata aatccagttt gatttatatg cacccttcta tttgttacac gaatctggcc
      361 gtctgcaata gaccagtttc cagaaacttt actaacggaa atatattgaa atgctccatc
      421 tccttttgat attgtatatg taactgtacc agatgctttt acaccaccag ttttatccca
      481 tccatcttct gatatacttg cttttctaag aggctttatt gtattagtat caaaaaaact
      541 gtcttagaat atatctttga tgttaaattt ttagattgtt taacttctaa taattgagtt
      601 gttgatatag aatcaacgta tatggactct cctgttcttg catttttgat tatacctttt
      661 tcattggaac ttttaacatc agtaattcca tttttagcca tatcgtataa tttgtccatg
      721 tctgttactt gtttctctga atacattact ttcatagatg aatcttctac cttactagca
      781 cttgcatttg ttggtaatag tattgtcatt atgagtaaag ataaaatagt tgttacagat
      841 ttttttaacc tataattcat attaatcctt actttttatt tgttataaca gacattctat
      901 tgtaatagaa tataaatcca caatattttt aaaattagta ttgaataact attctcatgt
      961 tataattatt caaaggaggg attgatatga ggaaaaaatt ttttataggt attcttatag
     1021 ttttaataat ctctgtgata gctatactta atataaaaaa tgataaaaat gtctttattg
     1081 gagaatcaga aaattggaaa gctaaatttg taactactga aggacactca aaattagaaa
     1141 taaaatataa aaatagtaaa gatgtagttg aacaaaaatt taattattcc tatcaattta
     1201 tagatggtga agatgcagtt gtagttgtaa aaaatgaaat tttagaagca ggtcaagaaa
     1261 aagcaataaa aatacaaaga ccaggtcttt attcaaatag agaatctagt tacaagctaa
     1321 ttatatcttg gaatggaaat gaggaaacta taaacttgaa aaagaaataa ccatattatc
     1381 aatttttaaa aaattattat tcaaattctt ataaaaagag tgtttagaaa aatgaaacac
     1441 tcttttttgt ataagttttt gaaaactata aatgaaatat atcaaaatcg ttcgaaaaat
     1501 tactgaaata aactcttaaa tatattataa attaaggtat attatacatg aatttattgg
     1561 gagtttatga gttttaaaat tggaaaccaa acaataaggg ctaaaaatac tttaaggaaa
     1621 atatataaag ttctataaat tatcaaaaat atttcgcaaa aattgataat tattacaaat
     1681 aaattgataa tactaataat attagaaata tcgaaattga acaaactagg attaaagctc
     1741 aattagaaaa atccactaat cctgagaatg ctttgttatt ctcatttatt actgttgttt
     1801 tttcattatt tgctaattta tttttcagtt cattaaatgt taaatataac tctataaatt
     1861 taattt
//
LOCUS       DAMJWP010000354         1825 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.361, whole genome shotgun sequence.
ACCESSION   DAMJWP010000354 DAMJWP010000000
VERSION     DAMJWP010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.361"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            94..663
                     /gene="mocA"
                     /locus_tag="PC700_003595"
     CDS             94..663
                     /gene="mocA"
                     /locus_tag="PC700_003595"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HDF2582837.1"
                     /translation="MINAVIMASGFARRMGANKLLLNYKGCSIIEHVFREVSKIDFHQ
                     VVVVSQYEEVLLLCNKYGFEYIDNKNANIGQSESIKLGVLNSSKCDGYMFFVGDQPFI
                     DNLYIDKIINTFKLDRDFIVIPRYKDMCGNPVIFPYSKKEELLALKEDEKGKTILKNS
                     SKIKYVEVPKEMLFDIDTKMDYERIGGKL"
     gene            660..1679
                     /locus_tag="PC700_003596"
     CDS             660..1679
                     /locus_tag="PC700_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HDF2582838.1"
                     /translation="MKLIKTIDAVGQVLCHDITQIIPGEFKGRKFKKGHIVKEEDIPV
                     LLSLGKDNLYVWEKSEGMVHENEGALFLKELTAGENLDFSEIKEGKIDFIAACDGLLK
                     IDVDALFDLNCVGEIMMATLHNNFIVNKGLKVAGTRVIPLVIDEKKLEDAKKVVGNRK
                     IVNVVPFKPKKVGIVTTGNEVFYSRIVDKFGPVIEEKVKGFGCEVIGQTICPDDKEII
                     KSAIKEFISQGAELICCTGGMSVDPDDVTPTAIKETGADLVTYGSPILPGAMFLLAYY
                     GEVPIMGIPGCAMYHKTTVFDIVLSRVLIDEKLDKYDIARYGHGGLCMNCDVCTYPAC
                     NFAKI"
BASE COUNT          731 a          164 c          348 g          582 t
ORIGIN      
        1 tttatatgat attatatata tagataatat caatagagat attataaaaa tcatagaact
       61 atcaatatat tataaatacc aaggatgata tatatgataa atgcagtgat aatggcatca
      121 ggctttgcca gaagaatggg agcaaataaa cttttattaa attataaagg ctgttctata
      181 atagaacatg tatttagaga agttagcaag attgattttc atcaagttgt agtagtaagt
      241 caatatgaag aggttttgtt attgtgtaat aaatatggtt ttgaatatat agataataaa
      301 aatgcaaata taggtcaaag tgaatctata aaattaggag ttttaaatag ctcaaaatgt
      361 gatggttata tgttctttgt aggagaccag ccatttattg ataacttata tatagataaa
      421 attataaata cttttaaatt agatagagat tttatagtaa ttccaagata caaagatatg
      481 tgtggaaatc cagtcatatt cccatatagt aagaaagaag aattgttggc acttaaagaa
      541 gatgaaaaag gtaaaaccat tttaaaaaat tcatcaaaaa taaagtatgt agaagttcct
      601 aaagaaatgt tatttgatat cgatacaaaa atggactatg agagaatcgg gggtaaatta
      661 tgaagctaat aaaaactata gatgctgttg gacaagtttt atgtcacgac ataactcaaa
      721 taatacctgg agaatttaaa ggaagaaaat ttaaaaaggg tcacatagta aaagaagaag
      781 atattccagt gttattatct cttggtaaag ataatttgta tgtatgggaa aagtcagaag
      841 gaatggttca tgaaaatgaa ggcgcattgt ttttaaaaga actaactgct ggagaaaatt
      901 tagattttag tgaaataaaa gaaggaaaaa tagattttat agcagcatgt gatggtctat
      961 taaagataga tgttgatgca ctttttgatt tgaattgtgt tggagaaata atgatggcta
     1021 ctcttcataa taattttata gtaaataaag gtttaaaagt agctggaaca agagtgatac
     1081 ctttggttat agatgagaaa aaattagaag atgcaaaaaa agtagttggt aatagaaaaa
     1141 tagtaaatgt agtacctttt aaacctaaga aagtaggaat tgttactaca ggaaatgaag
     1201 tgttttatag cagaattgtt gataaatttg gaccagtaat agaagaaaaa gtaaaaggct
     1261 ttggttgtga agttatagga cagactatat gcccagatga taaagaaatt ataaagagtg
     1321 caataaaaga atttataagt caaggagcag aacttatctg ttgtactggc gggatgagtg
     1381 tagacccaga tgatgttacg ccaacagcta taaaagaaac tggagctgat ttagtgacat
     1441 atggttcacc aatattacca ggagctatgt ttttacttgc ttattatgga gaagttccaa
     1501 ttatgggtat accaggatgc gcaatgtatc ataaaactac agtatttgat atagttttat
     1561 ctagggtttt aattgatgaa aagcttgata agtatgatat agctagatat ggtcatggtg
     1621 gcctttgtat gaactgtgat gtatgtactt atcctgcatg taattttgct aaaatataaa
     1681 gttattaaaa aagagtattt acaaattaag tactcttttt aaatatgaaa caaaacagct
     1741 agaatgatgt tttaagaaaa gcatatttat ttagaaatta taataactta ttttcaatgt
     1801 gtaaaagaat gaataaagtt gcaat
//
LOCUS       DAMJWP010000355         1816 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.362, whole genome shotgun sequence.
ACCESSION   DAMJWP010000355 DAMJWP010000000
VERSION     DAMJWP010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.362"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            87..281
                     /locus_tag="PC700_003597"
     CDS             87..281
                     /locus_tag="PC700_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HDF2582839.1"
                     /translation="MSVIVMCSRCGKEIDKYRNKYITYYEKNTNAEIKVCINCALDLL
                     EKTRENQELNIREFQDTIIL"
     gene            complement(383..892)
                     /gene="vanZ1"
                     /locus_tag="PC700_003598"
     CDS             complement(383..892)
                     /gene="vanZ1"
                     /locus_tag="PC700_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ1"
                     /protein_id="HDF2582840.1"
                     /translation="MKSRKHNITKGLFIVYIIILTWIILFKLQFDISSLETMNLRSIN
                     LVPFAGSLIINNRVDISEIILNVAIFVPFGIYVCMLKEEWSFIKKVIPIFITSLAFET
                     LQYIFALGASDITDLIGNTLGGIIGIAVFMLLSKIFKNNTIKIINVLALIVTIIVVLF
                     LGLVIFANL"
     gene            complement(1212..1602)
                     /locus_tag="PC700_003599"
                     /pseudo
     CDS             complement(1212..1602)
                     /locus_tag="PC700_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902377.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
BASE COUNT          667 a          251 c          189 g          709 t
ORIGIN      
        1 tttgatacag ttttaacaat cattattttc agaaaagtga tactattgaa tatattaaga
       61 ataattagag atggaggaac taagatatga gcgtaattgt aatgtgtagt cgttgtggaa
      121 aagaaataga taaatataga aataaatata taacttatta tgagaaaaat accaatgcag
      181 aaattaaagt ttgtataaat tgtgcattag atttattaga aaaaacaaga gaaaatcaag
      241 aattgaacat acgagagttt caagatacaa taattcttta ataatttagg aaagcaccta
      301 tataggtgct ttttatgtgt aaataatttt atatgtaact gtatcttacg ttctatttta
      361 agctaaacct atatacttat ttctataagt ttgcaaaaat aactaatcct aaaaataata
      421 caacaataat tgtcacaatc aatgccaata cattgataat ttttatggta ttattcttaa
      481 atattttcga taatagcata aaaacagcta ttcctatgat tcctcctagt gtgtttccta
      541 tcaaatcggt tatatcactt gcacctagtg caaatatata ctgaagtgtt tcaaatgcta
      601 aactagttat aaaaataggt ataacttttt ttataaagct ccattcttct tttagcatac
      661 acacatatat cccaaatggt acaaatatag ccacatttaa tataatctct gatatatcta
      721 ctctattatt aattataagt gacccagcaa aaggtactaa gtttatgctt ctaagattca
      781 tggtttcaag agatgagata tcaaattgca acttaaatag tattatccat gttaaaataa
      841 ttatgtaaac tataaataat ccttttgtaa tattatgttt tctagatttc atttattacc
      901 tccttactat attattgtat tattttatta atacaataat atagtaaggt aatttatctg
      961 tcaactattt cttctaaaat tatattcctt ttataatttc aatatcttta tcatatttac
     1021 atgatttaag ctattgtagt atttcatata tctttaaatt ttaattggta ttattctcta
     1081 tattttttac tatcaaacac taaggaaata atcacttttc tttatataat gttttaccat
     1141 cacataaaac taacattaaa tccaaacgct actctcaatt gtctcaaact aaaaagatac
     1201 tgatagcttt attagtcatc aatatcttct tctttcaaat ttttaaagta ttcttctaat
     1261 ttgtccaagc tcttactatc taaaacttca tcatcatgta gtgctgaaat aaaactctta
     1321 aatgaattgt tatgcataaa actaaaaaaa ctctttgtct caacttttaa gtaatctttc
     1381 tcttttatag ctatagtata atgcgtacat ctatctacct taataagtag ttaaaaatcc
     1441 cttatctact aaagttttta aaaatgtcaa tgttgtgctc tttttccaat cgtatttcaa
     1501 cttcattgct tctatgatat ctcttgaggc taatacatca tctgctcccc aaatatactt
     1561 cattagtttt agttctgcat gtggtaattt attaatcttc attagtttac acctcttacc
     1621 aatttatgta taattaaatt ttatactaat aatagtatgt aatcaatcag attggaacaa
     1681 atataatatt ccaatttatt ccatataaaa ataattaata aaaaagaagt actctacttt
     1741 agagagtact tttggattat atattcaaaa atttatctaa catgattaga gtatgtttta
     1801 tgctttagtg tgataa
//
LOCUS       DAMJWP010000356         1796 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.363, whole genome shotgun sequence.
ACCESSION   DAMJWP010000356 DAMJWP010000000
VERSION     DAMJWP010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.363"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            59..505
                     /locus_tag="PC700_003600"
     CDS             59..505
                     /locus_tag="PC700_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2582841.1"
                     /translation="MDFKNLQYESLSYIICNLQKNFKLYCEKCLKPYKLTNGLYFYLI
                     YINKNRNCSLNDVSTEFEVDKAHTTRTISRLEQDGYIEKIQNPNDSRAFQLRVTDKGE
                     EVLGDIKNIFSKWDNHIKKEFSDTEYKELVKNLHVVKDIKTAVEEG"
     gene            507..1601
                     /locus_tag="PC700_003601"
     CDS             507..1601
                     /locus_tag="PC700_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HDF2582842.1"
                     /translation="MYCNLLKPIKIGNLELKNRVSFAPTSMGLKLEEKIKKFSDIAKS
                     GVALITLGDVSIRPSFHKVAISLSDEDGVIKYKKIVDEIHNSGAKVSAQLFCSDYDVN
                     LIKDTMKMGITSHDEIKKIMNDGVKDYITNMPKEEIKNIINLFKVTALNAKKAGFDMI
                     QIHGDRLVGSFSSSIFNNRNDEYGGTCDNRSRFASEIISSIRDEVKDIPIDYKFAIRQ
                     ENPHYGNAGVLLSEVEYFVKKFESLGVNSFHVTLANHSKLEDTIPTNNHPYFKDEGCF
                     LYLADEVKKHTNLPVCGVGKLSSPDFIESIISNNRVDMVSMSRQLLADSNWLQKVKDG
                     RVDEIKKCCYCNKKCADALQTRSQFGCILD"
     gene            1689..>1796
                     /locus_tag="PC700_003602"
     CDS             1689..>1796
                     /locus_tag="PC700_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDF2582843.1"
                     /translation="MKSLVLYSSLTGNTKKIAYAIYDEIQEEKDIKDVNE"
BASE COUNT          732 a          175 c          324 g          565 t
ORIGIN      
        1 ttaatataat atagttgata tagtcaaaca tattataatt atagaaggga aatttaagat
       61 ggattttaaa aatttacagt atgaaagttt gtcatatatt atttgtaact tgcaaaaaaa
      121 ctttaaactt tattgtgaaa aatgtttaaa accatataaa cttacgaatg gtctatattt
      181 ctatttaatt tatattaata aaaataggaa ttgttctttg aatgatgttt ctacagaatt
      241 tgaagttgat aaagctcata caacaagaac tatatctagg ttagaacaag atggatacat
      301 agaaaaaatc caaaacccta atgatagtag agcttttcag ttgagagtaa cagataaggg
      361 tgaggaagta ttaggggata taaaaaacat attttcaaag tgggataacc acataaaaaa
      421 agagttctca gatacagaat ataaagaact ggtaaagaat ttacatgtgg taaaggatat
      481 aaaaacagct gtggaggaag gataggatgt attgtaattt attaaaacca attaaaattg
      541 gaaatttgga attaaaaaat agagtatctt ttgcacctac aagcatgggt cttaaattgg
      601 aagaaaaaat taaaaagttt tctgatattg ctaagtcagg agtagctctg attactctag
      661 gagatgtatc tattagacct agttttcata aggtagctat aagcctatcg gatgaagatg
      721 gagtcattaa atacaagaaa attgtagatg aaatacacaa ttctggtgca aaggtatcag
      781 cacaactatt ttgttcagat tatgatgtta atttaattaa agatactatg aagatgggaa
      841 ttacttcaca tgatgagata aaaaaaataa tgaatgacgg agtaaaagat tacataacaa
      901 atatgcccaa agaagaaatt aaaaatatta taaatttatt taaagtaaca gcattgaatg
      961 caaaaaaagc aggttttgac atgattcaaa ttcatggtga taggcttgta ggaagttttt
     1021 catcaagtat atttaataat agaaatgatg aatatggtgg aacgtgtgat aatagaagta
     1081 gatttgcaag cgaaataatt agtagtatcc gagatgaagt taaggacatt ccaatagact
     1141 ataaatttgc aataagacaa gaaaatcctc attatggaaa tgcaggagtt ttactttcgg
     1201 aagttgagta ttttgttaaa aaatttgaat ccctaggtgt aaacagtttt catgtaacac
     1261 ttgcaaatca ttcaaaatta gaagatacta taccaaccaa taatcatcct tactttaaag
     1321 atgaaggctg ttttttatat ttagctgatg aagtaaagaa acacacaaat cttccagtat
     1381 gtggggttgg aaaattgtct agcccagatt ttatagaaag tattatttca aataatagag
     1441 tagatatggt ttctatgtca agacaattac tagcagatag taattggtta cagaaagtaa
     1501 aagatggaag agtagatgag attaaaaagt gctgttattg taataagaag tgtgcagatg
     1561 ctttacaaac acgctctcag tttggatgta tactagatta gaatatcttt ataaaaataa
     1621 ataatttgtt aaggttaata tatttttggt gatttagata agtatttaga taaagaggag
     1681 gagacatcat gaaaagttta gttttatatt caagtttaac tggaaatact aaaaagattg
     1741 cttatgctat atatgatgaa attcaagaag aaaaagacat taaagatgta aatgaa
//
LOCUS       DAMJWP010000357         1779 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.364, whole genome shotgun sequence.
ACCESSION   DAMJWP010000357 DAMJWP010000000
VERSION     DAMJWP010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.364"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     repeat_region   716..1673
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=716..744
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaag"
BASE COUNT          677 a          173 c          276 g          653 t
ORIGIN      
        1 acaaagagtg acttacgagt tgaatttaaa tttatattta aatccaaaaa ataaactctg
       61 tttatgatga actccactct aacgcaaaat agattgtagt tcttcttgct tttattatac
      121 cacaaaacat tataaataaa actgttgttt ataaaaaaat gcaaagtaaa attttttata
      181 aacaatgata aaaattttat tatagagcta ttaacttatg aaatatctgt aaatgagtag
      241 atatttcata agttaatatt tattgtttgt ataaaatatt tggtaaaaat aagtaagttt
      301 tatatgttat aataattgta gcaagaataa taatcgaaag cagcaagcat gtcagctggt
      361 gttatatcag tttatattta tgataaaata aaaatctccc aaacgccaat aaggatgatt
      421 taaaaaaata atattttcac ttaaaaataa tcactcttta taggagtaaa ttattttctt
      481 gattttatta tactacaaat tggtacagat atttaaaaat aatatattca taatattata
      541 aaattataaa tagttttgca gtgagcgata tttttgataa aatagggcct aacagttgaa
      601 atataaggca ttgagggtat gtgataaatg ttatcatttg cactactcat ggttcactgc
      661 aaatttgaga gaattgtata gatgtaagtg ttggaaatac tcaatttatt ttggggtttt
      721 atattaacta agtggtatgt aaagtctcca tctccaatta acataaataa ttcatccgct
      781 ggttttatat taactaagtg gtatgtaaag ccgcttggca aatcataaga aggattattt
      841 tctccaacgt tttatattaa ctaagtggta tgtaaagaaa ataaaagagt ttgaattacc
      901 cattttttca tggttttata ttaactaagt ggtatgtaaa gtacgacatt gaataaaatg
      961 ggtacagaga caatttataa gttttatatt aactaagtgg tatgtaaagt ttataaatct
     1021 tttaatgact gtctctgatg gtacaggttt tatattaact aagtggtatg taaagaaagg
     1081 aatagatgat atgattgcta atgctgaaca acaagtttta tattaactaa gtggtatgta
     1141 aaggggtctg actttaatat atatatatat atatataaat tgttttatat taactaagtg
     1201 gtatgtaaag ttataaactc ccacgacaaa attaactata acctcgtgtt ttatattaac
     1261 taagtggtat gtaaagagtt ttaaagaggg cagacctaaa aaatatacta aaagttttat
     1321 attaactaag tggtatgtaa aggcagaaga tttagcgact gttgttgctg aatgggatgg
     1381 ttttatatta actaagtggt atgtaaagat taaattgctc agtctagtac ttaaagaatt
     1441 ataaggtttt atattaacta agtggtatgt aaagttgcta tgatttaatt atacgacgtt
     1501 ttgtctttaa aggttttata ttaactaagt ggtatgtaaa gtgctgtgcc acaatttgat
     1561 gcttctatag ctaaattcgt tttatattaa ctaagtggta tgtaaagctt ttaagtgcat
     1621 caaaggagtt acaagctata tttagtttta tattaactaa gtggtatgta aagacagatc
     1681 taatatcaaa ataaggttat gacttttaaa agcatagcca tatcagtaaa aatttaatta
     1741 tcatttaaaa aataaatttt tatttaaaga atacacata
//
LOCUS       DAMJWP010000358         1775 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.365, whole genome shotgun sequence.
ACCESSION   DAMJWP010000358 DAMJWP010000000
VERSION     DAMJWP010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.365"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            130..1179
                     /locus_tag="PC700_003603"
     CDS             130..1179
                     /locus_tag="PC700_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2582844.1"
                     /translation="MFKKIVAIASSIIVSTICLTGCDFNSNDKKDESSSSKNLDKVTI
                     AEVTHSVFYAPQYAAITKGFFEDEGIKIDLINTQGADKTMAALISGEANVGLMGPEAS
                     IYVYNQNKNDYAVNFAQLTKRDGSFLVARKKMPNFSYNDLKGTEILGGRKGGVPLMTF
                     EYVLKEKGLTIGENLKAGNVNVRTDVQFGVMAGAFAGGEGDFTTAFEPTATAMEKEGS
                     GYIVTSIGKDSGEIPFTAYSTTRSYMKNNKDLIQRFTNAVYKGQLWVQQASSKEVAKA
                     MQPFFEDISLEDLTTVVDRYKSIDAWSQNPVLKEEGLKKLMTVMKEAKELDKEAPYKD
                     IVNTEFANNAIKNIK"
     gene            complement(1329..1676)
                     /locus_tag="PC700_003604"
     CDS             complement(1329..1676)
                     /locus_tag="PC700_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDF2582845.1"
                     /translation="MHENKKNLLDGSLAQYNDTAVPKIPVFAGNDITSEVYYFKPNQV
                     LNAHRHPNGEQIFVFLKGEGKMKLGEHECDVKNGDTVFVPTGEWHEITNGSNEEMVAV
                     QITKINAGAEYRG"
BASE COUNT          653 a          242 c          282 g          598 t
ORIGIN      
        1 taaacataaa actgaaaatt attattaagt ttttgtagaa atattataag catagataca
       61 tttttgtaac aactacaata aatttttaat actatatact attcgtatgt cttatgggag
      121 gtatttatta tgtttaaaaa aatagtcgca atagcaagct ctatcattgt aagtacaata
      181 tgtctaactg gatgtgattt taactctaat gacaaaaaag atgaatcttc ttcgtctaaa
      241 aatctggata aagtaactat agcagaagtt actcactctg ttttttatgc tccacagtat
      301 gctgctatta cgaaaggatt ctttgaagat gaaggtataa agattgacct tataaatacc
      361 caaggtgcag ataaaactat ggcagctttg atttcaggtg aagcaaacgt aggtttaatg
      421 ggtcctgaag catctatata tgtatataat caaaacaaaa acgattatgc agtgaacttt
      481 gctcaattga caaaaagaga tggtagtttt ctagttgcta ggaaaaaaat gcctaatttc
      541 tcttacaatg atttaaaagg aacagaaata ttaggtggta gaaaaggtgg agttccacta
      601 atgacttttg aatatgttct aaaagaaaaa ggtcttacta ttggagaaaa tctaaaagca
      661 ggcaatgtta atgtaagaac tgatgtacaa tttggtgtaa tggctggagc ttttgcaggt
      721 ggagagggtg actttacaac agcatttgaa cctacagcaa cagcaatgga aaaagaaggt
      781 agtggatata tagttacatc tataggaaaa gattctggag aaataccttt tacagcatac
      841 tctactacga gatcatacat gaaaaacaat aaagatttaa ttcaaagatt tacaaatgct
      901 gtttataaag gtcaattatg ggttcaacaa gcatctagca aagaagtagc taaggctatg
      961 caacctttct ttgaagatat atctttagaa gatttaacaa cagttgtaga tagatataaa
     1021 tcaatagatg catggtctca aaatcctgta ttaaaggagg aaggtttgaa gaaattgatg
     1081 actgttatga aagaagctaa agaactagat aaggaagcac cttataaaga tatcgtaaat
     1141 actgagtttg caaacaatgc tataaaaaat attaaataaa atatattagt tttagctaga
     1201 tattttcttg tttttataat gaaaattaac aaaaatataa aaagagcctt ttaaaagact
     1261 ctttttatat ttgagattaa agtttatata ataaaataga tattaaagta ctaactgtac
     1321 ttatatatct atcctctgta ttctgctcca gcattaattt ttgtaatttg aacggctacc
     1381 atttcttcat tgcttccatt agtaatttca tgccactctc ctgttggtac aaatacagta
     1441 tcgccgtttt ttacatcaca ttcatgctca ccaagtttca ttttaccttc tccttttagg
     1501 aacacaaata tttgttcacc attagggtgt ctatgagcat taagaacttg attaggttta
     1561 aaataataaa cttctgaagt tatatcgtta ccagcaaata ctggtatttt tggaactgct
     1621 gtatcattat attgagctaa gcttccgtct aatagattct ttttattttc atgcatacta
     1681 gaaatacctc ttttctatat tatagattta ctattattat accaatataa gaaaatagta
     1741 ttctcacttt acacaatagg aataaaattc tttta
//
LOCUS       DAMJWP010000359         1769 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.366, whole genome shotgun sequence.
ACCESSION   DAMJWP010000359 DAMJWP010000000
VERSION     DAMJWP010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.366"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(119..1663)
                     /locus_tag="PC700_003605"
     CDS             complement(119..1663)
                     /locus_tag="PC700_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HDF2582846.1"
                     /translation="MRNLQKKLMFFLFICIILQITYTTPVYSLCKLYKNNSINNFESI
                     VSNNQFISKYAIQIILIMAIICMMLIFYIIYDKLNFKIKLQKIAYTDNLTGANTIDKF
                     VIDANKILCKNTQVKYALLYIDIDKFKYINDLFGYEVGNEILRNLTKIIKSNIFEEEM
                     FARISADNFIIIMKYIEEEDITKRLKTIFEELDLFNNNQEEKYKLVLSCGIYFILPED
                     RDINSIIDRANIPHKMAKGGHKSSYAFYDNKIHDQEIKEKEMENTMFSSLENKEFIIY
                     LQPKIDLNTGEIQGSEALVRWKRPDKGLIPPNEFIPFFERNGFVINLDLYVLEEVCIY
                     LRKWIDAGINPVTVSVNVSRIHLYCNNFIETYKNIIDKYNIPAKYIELELTESIIFDN
                     FDILIDIMNNLKKIGFLISMDDFGSGYSSLNMLKEIPMDILKLDQKFIMETYNSKRSK
                     IIVTKVIEMAKELGMKVISEGVETEEQFKLLKEVKCDMAQGYLFGKPMPIEEFEHLIV
                     SNLVRG"
BASE COUNT          613 a          270 c          161 g          725 t
ORIGIN      
        1 acaaagtata tgtcaattac tttttagtaa aagtataaaa aatttcacaa aaaaaatgaa
       61 cactacatta gagtccattt ttaacacaat ttattaaatt ctttatatta cagattaatt
      121 accctcttac caagttggat acaattaaat gttcaaattc ttcaattggc attggctttc
      181 caaacaaata tccttgtgcc atatcacact ttacttcttt taaaagttta aactgttctt
      241 ctgtctcaac tccttctgat atgactttca tacccaactc ttttgccatt tctattactt
      301 tagtaacaat tattttgctt cttttactat tataagtttc cataataaat ttttggtcaa
      361 gttttaagat atccatagga atttctttta acatatttaa tgatgagtac cctgaaccaa
      421 aatcatccat agatattaaa aatcctattt tttttaaatt attcattata tcaattaaaa
      481 tatcaaaatt atcaaatatg atactctccg ttaattcaag ttctatatac tttgcaggta
      541 tattgtattt atcaattata tttttgtatg tttctataaa attattgcaa tataaatgta
      601 ttcttgaaac attaactgaa acagtaactg gatttattcc tgcatcaatc cattttctaa
      661 ggtatataca tacttcttca agcacatata aatctaaatt tataacaaaa ccgtttcttt
      721 caaaaaatgg gataaattca tttggaggta ttagcccttt atcaggacgt ttccacctaa
      781 caagtgcttc tgaaccttgt atctctcctg tattaagatc tatcttaggt tgtaagtata
      841 taataaactc tttattttct aaacttgaaa acattgtatt ttccatctct ttttctttta
      901 tctcttggtc atgaatctta ttatcataaa atgcatatga acttttatgt cctccctttg
      961 ccattttatg aggtatatta gccctatcta tgattgagtt gatatctcta tcttctggaa
     1021 gtataaaata gattccacaa cttaaaacta acttatactt ttcttcttga ttattattaa
     1081 acaagtctaa ttcctcaaat atagttttta acctctttgt tatatcttct tcttctatat
     1141 atttcataat tataataaaa ttatccgcag atattcttgc aaacatttcc tcttcaaata
     1201 tattactctt aataattttt gttaaattac gcaaaatttc attcccaact tcatatccaa
     1261 ataaatcatt tatatattta aatttatcaa tatctatata taataaagca tactttactt
     1321 gagtattttt acatagtatt ttgttagcat caattacaaa tttatctata gtatttgctc
     1381 ctgtaagatt atctgtatat gctatctttt gcaatttaat tttaaaattt aatttgtcat
     1441 atattatata gaatataagc atcatacata ttatagccat aataagtatt atttgtattg
     1501 catatttgct aataaactga ttattagaca caatagactc aaagttattt atggagttgt
     1561 ttttatagag tttgcataaa gaatagactg gtgttgtgta tgtaatttgt aaaattatac
     1621 aaataaataa aaaaaacatc aatttttttt gtaaattcct cataaaaatt tcctcccatt
     1681 aaaacacctc tattttatta gcaaataagt acatctcaaa agtttccatt tcttgtaaaa
     1741 tggatagttt tgaaaagaaa atattaaaa
//
LOCUS       DAMJWP010000360         1758 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.367, whole genome shotgun sequence.
ACCESSION   DAMJWP010000360 DAMJWP010000000
VERSION     DAMJWP010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.367"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            283..738
                     /gene="smpB"
                     /locus_tag="PC700_003606"
     CDS             283..738
                     /gene="smpB"
                     /locus_tag="PC700_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDF2582847.1"
                     /translation="MAGKKILATNKRARHEYFIEETYECGIELKGTEVKSIRQGKVNL
                     SDGFASVDNSEVFLKQVHISPYEQGNIFNVDSLRVRKLLLHKHEIRKLIGATTIKGYS
                     LIPLSLYLKNGKVKVEIALAKGKKLYDKRQDMAKKDAQRRIEREMTGKY"
     gene            896..1245
                     /gene="ssrA"
                     /locus_tag="PC700_003607"
     tmRNA           896..1245
                     /gene="ssrA"
                     /locus_tag="PC700_003607"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          667 a          202 c          311 g          578 t
ORIGIN      
        1 atttataata tgtaatattt ttgttttgaa atttagaaat cagtgttact ggttaaagtt
       61 agtttagtat taagtatatt cgaagtagtt ttttagctat tttcatatct ttatattact
      121 taattatata tagcctaact aacttaatat atcagtgaaa tattgactaa aatgcctaaa
      181 tatgttatca tacaagttag tcaaataatt tggcaacatt aaatatttag taaatttcta
      241 acatttatgg aataaatttc taacaaagaa ggtgagattt atatggctgg taaaaaaata
      301 ttagcaacaa acaaaagagc aagacatgaa tattttatag aagaaactta tgaatgtgga
      361 attgaattaa aaggaacaga agtaaaatca ataagacaag gcaaagttaa cctaagtgat
      421 ggatttgcat ctgtagataa tagtgaagta tttttaaaac aagttcatat aagtccatat
      481 gagcaaggaa atatattcaa tgttgattct ttaagagtta gaaaactatt acttcataag
      541 catgaaataa gaaaacttat tggagcaact actataaagg ggtattcact tataccactt
      601 agtttgtatc taaaaaacgg taaagttaaa gtagagattg ccttagcaaa aggtaaaaaa
      661 ctatatgata aacgtcaaga tatggctaag aaagatgcac aaagacgtat agaaagagaa
      721 atgacaggta agtattaaac tagataaaac tgtatttttt agaataaaat tctttattat
      781 ctagaacata acattgattt ttattcttaa aagtattata ttatatatac attaaaaatt
      841 taataaaaat ggtgataaag tttgcgaaaa gtctactgga ctataaacta ctcaaggggg
      901 cgtaaaggtt tcgacgtggg tttggaactt gaggctgcat gtcgtgttac tctgggtcac
      961 gtaaaaactg gggaacttta aaataaacgc agacgataat ttcgcgatag cagcctagtt
     1021 gctgctcgtc cctcttagtc ctcctgccga ctaagactgg acgtcattat gcagggaact
     1081 actttagggg tgtctcgacc tgaagtagat taattgggac tggctaagca tagagcctgc
     1141 cacttggcgc tatgtgaggt gagatattag tgaagttggc taagcatgta gatgcagcaa
     1201 ggaaaagatt tgcggacagg ggttcgactc ccctcgtctc caccaatttt taaaaaagaa
     1261 tgatgaaata cttggaaata ctagtcttta ttgactagtg ttttttgttt tggagatgaa
     1321 atccaccaac cgtgccccaa ccacagaaaa agagattttt ttggggtata ttggggtgca
     1381 tttgattaat ccatggaaca tataaagaat acttttagag atatagtgaa tttgatgata
     1441 atacaataaa tattttatac tgatgaattg tttttatatt atgcttttta tttcaatcta
     1501 ggaaatatat aacttgttta aacttatgat taaaaggata aagaagttta aagcatataa
     1561 gtgctactta atctattaga gtaattacta gttataatga tatacagaat cctataagat
     1621 tatgtataag tatagaacaa atttattatc tatgtgggat gctaatttaa attttttaat
     1681 tgtatttaaa aaaataagat gtaaaaaagg aattgaaaaa tgttaagata ataaacacat
     1741 agaattatta agtttaat
//
LOCUS       DAMJWP010000361         1754 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.368, whole genome shotgun sequence.
ACCESSION   DAMJWP010000361 DAMJWP010000000
VERSION     DAMJWP010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.368"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            152..1732
                     /locus_tag="PC700_003608"
     CDS             152..1732
                     /locus_tag="PC700_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HDF2582848.1"
                     /translation="MLIGTSKLSRNVDTACVEEVGIPMIVLMENAVISAMKNMDIDMY
                     NSYTVVCGVGNNGGDGLGIARHLNTYGKEVNVFLVGNLEKLSECSKINYNVLLNMGIN
                     IININKNDLSEKNYGDENEVDKIIKVEKQNIIKNKNLTIFKDRVSKSDVVVDAIFGTG
                     LKRDITGIFKEVIDIVNDNSKNTYSIDIPSGIDSDNGNVLGTCIRANKTISFEFYKRG
                     FLNYDTSTLIGDVVVEKIGVPDFITRKYHDEEFITDKTFVKNNIRRRDKHGFKSDFGK
                     VSIVAGSKGFYGASFIATESAVKSGSGLVTLISSEDVQEKVSIRLTEAMTLNFEEERL
                     NKLLNSSDAIGFGPGMGDNSQTFDKLLKIVENSNCPIVLDADGLNVMKDRCYKFLEWK
                     NRFVITPHLGEMARLTGDSIGYIREHRVDVAKEFAQKYNLVVLLKGYQTVITDGKKTY
                     INPTGNSCMATGGMGDCLLGMITSFIGQGMDILEATVSGAYIHGYIGDKLSKAMYTVN
                     ATDLISNISLTMKELLDI"
BASE COUNT          684 a          146 c          349 g          575 t
ORIGIN      
        1 tgttttaatt caaaattttt tgttatttta aattatttca aaactcttag ttttagtgat
       61 tttatttatg atatttatgg gattaaaaga tttcaataga tatttttggt aagtatttaa
      121 gaagtcttta actttatagg agtgataatt tatgctaata ggtacatcta aattaagcag
      181 aaatgttgat actgcatgtg tagaagaagt agggattcca atgattgtat tgatggaaaa
      241 tgcggttata tctgcaatga aaaatatgga tatagacatg tataatagtt atacagttgt
      301 atgtggtgtt ggaaataatg gaggagatgg tcttggaata gcaagacatc taaatacata
      361 tggaaaagaa gtaaacgtat ttttagtagg taatcttgaa aaactaagtg aatgctctaa
      421 aataaattac aatgtacttt taaatatggg aattaatatt ataaatatta ataaaaatga
      481 tttaagtgaa aaaaattatg gagatgaaaa tgaagtagat aaaattatta aagttgagaa
      541 acaaaatatt ataaaaaata aaaatttaac aatttttaaa gatagagtat ctaaaagtga
      601 tgttgtagta gatgctatat ttgggactgg attaaaacgt gatataactg gtatttttaa
      661 agaagttatt gacatagtaa acgataatag caaaaataca tattctatag atattccttc
      721 aggtatagat agcgataatg gaaatgtatt aggaacttgt attagagcaa acaaaacgat
      781 tagctttgag ttttataaaa gaggttttct aaactatgac actagcacac ttataggtga
      841 tgtagttgta gaaaagatag gtgttccaga ttttattaca agaaaatacc atgatgaaga
      901 gttcataact gataagactt ttgtaaaaaa taatattaga agaagagata agcatggttt
      961 taaaagtgat tttggaaaag tatctatagt tgcaggttca aaaggatttt atggtgcaag
     1021 tttcatagca actgaatctg ctgttaagag tgggagtgga cttgtaacat taattagtag
     1081 tgaagatgtg caagaaaagg tatctattag acttacagaa gcaatgactt taaattttga
     1141 agaagaaaga ttaaataaat tacttaattc tagtgatgca attggttttg gtccaggtat
     1201 gggagataat agtcaaacat ttgataaatt acttaagata gtagaaaatt ctaattgccc
     1261 aatagtatta gatgcagatg gattgaacgt tatgaaggac agatgttata agtttttaga
     1321 atggaaaaat agatttgtaa ttacacctca tttgggagaa atggccaggt tgacaggaga
     1381 ttcaattggg tatataagag aacatagagt agatgttgca aaagaatttg ctcaaaaata
     1441 taatctggtg gtgcttttaa aaggttatca aactgtcata acagatggta aaaaaacata
     1501 tataaatcct acaggaaata gctgtatggc aactggtgga atgggtgatt gtttgcttgg
     1561 aatgattact tcatttatag gtcaaggtat ggatatattg gaagctacag ttagtggagc
     1621 atatatacat ggctatatag gagataaatt gagcaaagca atgtatacgg taaatgctac
     1681 ggatttaata agtaacattt cattaacaat gaaagagttg ttggatatat aattgattaa
     1741 aaaatagcta cgca
//
LOCUS       DAMJWP010000362         1745 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.369, whole genome shotgun sequence.
ACCESSION   DAMJWP010000362 DAMJWP010000000
VERSION     DAMJWP010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.369"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            191..1003
                     /locus_tag="PC700_003609"
     CDS             191..1003
                     /locus_tag="PC700_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HDF2582849.1"
                     /translation="MADCNSCPSKGNCNSQSNCSIENNPNNKFGKIIGVMSGKGGVGK
                     STVTALLANKLNKMGYKVGILDSDITGPSIPRLMGVKNVKAYSDGSYIYPVENSNNIK
                     VMSINLMIDDENEPVVWRGPLLGGVVKQFYTDVLWEELDYLLIDMPPGTGDVALTVMQ
                     SIPISGIVMVSVPQDLVSMIVSKAVNMAKKMNINVLGVIENMSYIQCPDCSKKIKLFE
                     GESTEKFLDDLDLELLGELPMTKEIIDITHNGVTEISDDLDSILTNVVEKIK"
     gene            complement(1092..1283)
                     /locus_tag="PC700_003610"
     CDS             complement(1092..1283)
                     /locus_tag="PC700_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582850.1"
                     /translation="MERLMELIERKFLKDVELLELEDIENVIECKMTQPYNAHYNEYL
                     VKVKNGDEVEEYFVYLKHR"
     gene            1588..>1745
                     /locus_tag="PC700_003611"
     CDS             1588..>1745
                     /locus_tag="PC700_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2582851.1"
                     /translation="MFKKKSGYTFTNFLNKVRVEKNKKYLLKKDLSILDVTVLIGFNS
                     QNYYSIIF"
BASE COUNT          651 a          199 c          241 g          654 t
ORIGIN      
        1 attgaaataa tcactttttc aagaatgtta taattttttt attgtattct ttttgctata
       61 gtctatgaat aaatcaatag aaaatcttct aagttgcttt taaataatga ctttatggtt
      121 caattgtact aaaatagtat atgtaggtta caaataatct aataaatatt aaagtttgga
      181 ggtaatagat atggcagatt gtaattcatg tccatcaaag ggaaattgta atagtcaatc
      241 aaattgttct attgaaaata accctaacaa taaatttgga aaaataattg gtgttatgag
      301 tgggaagggt ggagttggaa aatcaactgt aactgcttta cttgctaata agctaaacaa
      361 aatgggatat aaggttggta tattagattc agatataact ggaccaagta ttcctagact
      421 tatgggtgta aaaaatgtaa aagcttattc tgatggctct tatatttacc ctgtagaaaa
      481 ctcaaataac ataaaagtaa tgtctataaa ccttatgata gatgatgaga atgaacctgt
      541 tgtttggaga ggacctttac ttggaggagt tgtaaagcaa ttttatactg atgtactttg
      601 ggaagaatta gactatttat taatagatat gcctcctggt actggagatg ttgccttaac
      661 ggttatgcaa tctattccta taagtggaat agttatggtt tcagtacctc aagatttggt
      721 atctatgatt gtatcaaaag ctgttaatat ggctaaaaaa atgaacataa atgtccttgg
      781 agttattgaa aatatgagtt atatccaatg tcctgattgt tctaaaaaaa tcaagttatt
      841 tgaaggagaa tctactgaaa agtttttaga tgatttagac ttagagcttt taggagaact
      901 tcctatgact aaagaaatta tagatattac tcataatgga gttacagaaa ttagtgatga
      961 tttagattca atattgacta atgttgttga aaaaattaaa taaataaaaa ttttataatt
     1021 ttatatttat caaaaaaaga ggcttgtatt tattttataa tcatacaaat aaatataaac
     1081 ctcttttatg tttatctatg ttttaaatac acaaaatatt cttctacttc atctccattt
     1141 tttactttta ctaagtattc attataatga gcattatatg gctgtgtcat tttgcattct
     1201 attacatttt ctatgtcttc taattctaaa agttctacat cctttaaaaa ttttctttct
     1261 atgagttcca ttaatctttc cataatatat cctcacttcc cctataatta agataaacta
     1321 tatatcctct taatacagag tttatctgtt tattatatta tatccaatat attacagttt
     1381 ctaacatcat tcataaatta ttttaacaaa tagagattga ttttttaata aatatgcctt
     1441 gataaaatga aacatagacc aactcttagt tttatgtaaa gaaaacttaa agaaaactat
     1501 agattatatt cacaaaaact actcagaaga tataacagta agtaatatat gtaatgaatt
     1561 acaacttaat aaaagttatt tttttctttg tttaagaaaa aaagtggata tacttttact
     1621 aattttttaa ataaagttag agttgaaaaa aacaaaaagt atctattaaa aaaagacttg
     1681 tctattttag acgttactgt actaattgga ttcaatagtc aaaattacta tagtataatt
     1741 tttaa
//
LOCUS       DAMJWP010000363         1719 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.370, whole genome shotgun sequence.
ACCESSION   DAMJWP010000363 DAMJWP010000000
VERSION     DAMJWP010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.370"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(256..1584)
                     /locus_tag="PC700_003612"
     CDS             complement(256..1584)
                     /locus_tag="PC700_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2582852.1"
                     /translation="MGSNELKKTLGVSAALSTVVGSVIGAGVFFKPQAVYTLTGGAPG
                     LGILAWLIAGIITITAGLTAAEVSVAIPKTGGMMVYIKEIYGDKLGFLTGWMQIVLFY
                     PGMMAALGVIFGEQASALIGSPSLLLPIAIGIIVIVAGLNMLGSKTGGVIQTVSTICK
                     LIPLILIMVVGFIKGGGNNPILTPMVGEGLSLGSVLGQVLIAILFAFDGWMNVGTLAG
                     EMKNPGKDLPKAIIGGLSVVMAVYFIINLAYLWVLPANELANYASPASAVAEVIFGSM
                     GGKIISVGILISVFGALNGFLLTGSRVAYTLATDKTLPKYGIFSKLNSAQVPANAIAL
                     VSVIASIYALSGQFNLLTDLAVFATWIFYVLTFIGVMKLRKTHPNIPREYKVPLYPIV
                     PIIAIASGIFVVVNQLCFAGMKTTMISIGGLVITAIGLPVYAYMTRGIKR"
BASE COUNT          615 a          326 c          200 g          578 t
ORIGIN      
        1 gatatcactt agtttgaaaa aaaaacactt taaaataaag tatcttaaag caaataattt
       61 tttagatgtt taattttata attcaaaaac taaaaataaa gtgaatacct taaaataaac
      121 tttttagttc atagaacact ctttttttat aaaataaaac atctctttac ccttttaaca
      181 ataaaaaaga gacttatctc tattttgaga cagtctcttt tattttaatt ataaagcatt
      241 tgtaaactac ttggtttatc tttttattcc tctagtcata taagcataca ctggtaagcc
      301 tatagctgtt attacaagac ctcctataga tatcatagta gttttcatcc ctgcaaaaca
      361 caattgatta actacaacaa atataccact tgctatagct attataggaa caataggata
      421 taatggaact ttatactctc ttggtatatt tggatgagtc tttctaagtt tcataacccc
      481 tataaaagtt aatacataga atatccaagt agcaaataca gctaagtcag ttaataaatt
      541 aaattgacct gacaaagcat atatagatgc aataacagat actaatgcta ttgcatttgc
      601 tggaacttga gcagaattta atttagaaaa tataccatac tttggtaaag tcttatctgt
      661 agctaaagta tatgccaccc ttgaaccagt tagtaaaaat ccatttaatg ctccaaacac
      721 tgaaattaat atacctacag atattatttt tccacccatt gaaccaaata taacttcagc
      781 aactgctgat gcaggagaag cataatttgc taattcatta gctggtaata cccaaagata
      841 cgctaaattt attataaaat aaacagccat aacaactgac aatccaccta ttatagcttt
      901 tggaaggtct tttccaggat tcttcatttc tccagctaaa gtaccaacat tcatccatcc
      961 atcaaaagca aataatatag caattaaaac ttgacctagt acactaccca ggcttaatcc
     1021 ttcaccaacc ataggtgtaa gtattggatt atttccacca cctttaataa atccaacaac
     1081 cattattaaa ataagtggta ttaacttaca aattgtagag acagtttgta tgactcctcc
     1141 agtttttgaa cctaacatat taagccctgc aactattact ataattccaa ttgctattgg
     1201 aagtaaaagt gatgggctac caattagtgc tgatgcttgt tctccaaata taacacctaa
     1261 tgctgccatc atccctggat aaaataatac aatttgcatc catccagtta aaaaacctaa
     1321 tttgtctcca taaatttctt ttatatatac catcattcca ccagtttttg gtatcgcaac
     1381 tgatacttct gcagctgtaa gaccagctgt tatagttatg attccagcta ttaaccatgc
     1441 caatatacca agacctggag ctcctccagt taatgtatag actgcttgtg gtttaaaaaa
     1501 tacgcccgct cctataacag aaccaacaac agttgataat gctgctgata cgccaagagt
     1561 ctttttcagt tcattacttc ccattctttt cctcctcatt aatcctattt aattttaatt
     1621 tcatagttaa ataagcatat attatgccaa tatatagaaa aatagctttt ttatttatta
     1681 aaataaaata aactctactt agctttcgta atcttttac
//
LOCUS       DAMJWP010000364         1652 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.371, whole genome shotgun sequence.
ACCESSION   DAMJWP010000364 DAMJWP010000000
VERSION     DAMJWP010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.371"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            91..822
                     /locus_tag="PC700_003613"
     CDS             91..822
                     /locus_tag="PC700_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582853.1"
                     /translation="MLNKKNVSLIVLVIVLSLFTAIFVFNNRNTNDKSKEIAKNNEEI
                     VPKNLEALSIEQLTKIRDKAIDKNLEGLREFGEDSVKQRKDSILNFEFGLVAFVNKNY
                     SNLTEKEKLIRQINIVNYENSINDIMDPSGSFFELANQSYEINEFMLKNKKNYKEWSY
                     EYIEFLIDHLDELCKFVDFDIKDVIDIIDPTIKTDLSEEQKKSLNDKLKKVSSSDTLS
                     EDIKKEIKNWENNLNSLNMNKNNSN"
     gene            1064..>1652
                     /locus_tag="PC700_003614"
     CDS             1064..>1652
                     /locus_tag="PC700_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDF2582854.1"
                     /translation="MFNKNEILKKLIEEYDVKTTTDIQDMLKDLFASTIHQMLEAELD
                     DHLGYDRYGNKNKNTKNSRNGYRNKNVKSDFGEITLNIPRDRNGDFEPKVISNYKNDI
                     SGIENQVIGMYSKGMSTRDISSHLKSIYGIDISHSLVAKITDRVLPLAHEWQNRPLDM
                     VYPIVFMDAIHYKVRSEGRIINKAAYIAIGVNLEGI"
BASE COUNT          713 a          156 c          247 g          536 t
ORIGIN      
        1 tattctttag aataatcagt aataaaggtt attctaataa actgagttat tttttcacaa
       61 ataaaatact tattaaaaag gagatttgat atgttaaaca aaaaaaatgt aagtttaatt
      121 gttttagtaa tagttttaag tttattcact gctatatttg ttttcaataa tagaaataca
      181 aatgataaat caaaagaaat tgctaaaaat aatgaagaaa tagtgcctaa aaacttagaa
      241 gctttaagta tagaacaatt aacaaaaatt agagataagg ctattgataa aaatttagaa
      301 ggtcttagag aatttggaga agattcagta aaacagcgta aagattcaat tttaaatttt
      361 gaatttggat tagttgcttt tgttaacaaa aattatagta acttaacaga aaaagagaaa
      421 ttaataagac aaattaatat tgttaactat gaaaactcta taaatgacat aatggatcct
      481 tcaggttcat tctttgaatt agcaaatcaa agttatgaaa ttaatgagtt catgttaaaa
      541 aacaaaaaaa attataaaga atggtcatat gaatatatag aattcttaat tgatcattta
      601 gatgaattat gcaaatttgt tgattttgat ataaaagatg taatagatat tattgatcct
      661 acaataaaaa cagatctttc agaagaacaa aagaaatctt tgaatgataa attaaagaaa
      721 gtgtcttcaa gtgatacttt aagtgaagac attaagaagg agataaaaaa ttgggaaaac
      781 aatttgaata gtttaaatat gaataaaaat aattctaatt aactgatagt gtaaaatgta
      841 cataattata aataaaatag ggatgtctta aaattgatta ttttctaatc taaatatttt
      901 aggcctatct aaaaacataa gattatggtc taaaatttga aattttagac catgctttat
      961 ctttacaata aatgggtctt taaaataaag tgtgtattct tctaaaaagt ggtgataatt
     1021 aaaatattaa atatcactat ataaaggaag gtgcacactt tttatgttta acaaaaacga
     1081 aatattaaaa aaacttattg aagaatatga tgttaaaaca actacagata ttcaagatat
     1141 gctaaaagat ttatttgcga gtacaattca tcaaatgtta gaagcggagc ttgacgacca
     1201 tttaggttat gatagatatg gcaataagaa taaaaatact aaaaactcta gaaatggata
     1261 tagaaataaa aatgttaaat cagattttgg agaaataact ttaaatattc caagagaccg
     1321 aaatggagat tttgaaccaa aggtaattag taattataaa aatgatatat caggaataga
     1381 gaaccaagtg attggaatgt actcaaaagg catgtctact cgagatatat caagccacct
     1441 taagagtatt tacggaattg atatatctca ttctctagta gctaaaatta ctgatagagt
     1501 tctaccttta gctcatgaat ggcaaaacag acctcttgat atggtttatc caattgtatt
     1561 tatggatgct atacattata aagttagaag tgaaggtaga ataataaaca aagctgcata
     1621 tatagccata ggtgttaact tagaaggtat aa
//
LOCUS       DAMJWP010000365         1643 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.372, whole genome shotgun sequence.
ACCESSION   DAMJWP010000365 DAMJWP010000000
VERSION     DAMJWP010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.372"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            619..1524
                     /locus_tag="PC700_003615"
     CDS             619..1524
                     /locus_tag="PC700_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P63C domain-containing protein"
                     /protein_id="HDF2582855.1"
                     /translation="MESKEKSILRATHFGIIKIGEKDLNCAVLENGSRIINKSTVFKA
                     FGRTKRGRKKDEIRVPSKPNLPSFIDAKNLSDYISDELEDMLINTIKYKTKNGRIVEG
                     YPAEIIPLICDVYLQARLDNKLAVQQEGLAIASEILVRSLSKIGIVALVDEATGYQYD
                     RDRDELQKILSMYISKEFLPWTKRFPDEFYRQMFRLKNWTYPRPSSKRPGIVGTYTNK
                     YVYDLLPPGVKEELQRVNPTVKPGQRKHKHHQFLTEDIGNDHLKNHLLKVITLMQASK
                     DWKDFNVLFNRAFNLPVQLEIEYDE"
BASE COUNT          681 a          200 c          215 g          547 t
ORIGIN      
        1 actttataat ttgaaacctc ttacactaat aaaatttgaa agtgctcatt attcaataca
       61 aacatgccta aaattatatt ttaattttta cactatcaaa aacatatata ttaaatttga
      121 cccacttaaa tacagtaaat taatttctac aacatgatat aattaaatta tgacaaaaga
      181 ctttgatatt tataaaaact ttccaacaga tgtctcttat aagatttttt tatataatct
      241 taaattaaaa aactcattaa tatgccccta ttgttattct gataaaatta gtacttataa
      301 tgatttattt cattgcaata tttgcaatag taagttttca attacaacta ataccattat
      361 gcataaaact aaactagact acagaaaatg gctacttgct attaatttat ttatatttga
      421 tgaaaatatt agctatagaa aattatcaaa aaatatcgtt gttaataaaa atactgcgta
      481 caagatgata aaaaaactca attatctata tacatatttt aaaattgata ttattagtct
      541 tataaatact aatttatcta atgaagagat actaagtaat atcttactta ttaaaataaa
      601 ataaaggatg gtgttaaatt ggaatcaaaa gaaaaaagta tattgagagc aacacatttt
      661 ggtattataa aaataggtga aaaagattta aattgtgctg tactagaaaa tggttcaaga
      721 atcataaata aaagtacagt attcaaagct tttggaagaa caaagcgtgg tagaaaaaaa
      781 gatgaaattc gtgtaccttc taagcctaat ctaccaagtt ttattgatgc aaagaatcta
      841 tctgattata tttcagatga attagaagat atgcttataa atacaataaa atataagact
      901 aaaaacggaa gaattgtgga aggttatcca gctgaaataa tacctcttat atgtgatgta
      961 tatttacaag caaggcttga taataaatta gctgttcagc aagaaggact agctatagca
     1021 agcgaaattt tagtaagaag tttatccaaa atagggattg tagcattagt tgatgaagct
     1081 acaggatatc aatatgatag agatcgtgat gagctgcaaa aaatattgtc tatgtatatt
     1141 tctaaagagt ttttaccttg gacaaaaaga tttcctgatg aattttatag acaaatgttc
     1201 agattaaaaa actggacata ccctagacca agttctaaga gacctggaat agttggaaca
     1261 tacacaaata aatatgttta tgatttatta cctcctggag ttaaagaaga acttcaaaga
     1321 gttaacccca cagtaaaacc tgggcaaaga aaacataaac atcatcaatt tttaacagaa
     1381 gatataggta atgatcattt aaaaaatcat ttattaaagg ttataacctt aatgcaagca
     1441 tcaaaagact ggaaagattt caatgtgctt tttaatagag ctttcaatct acctgtacag
     1501 ctcgaaatcg aatatgatga ataatagact atagccttta acaacgcttt aaatactttt
     1561 aatttatatt ataaaaatag aattaactta tatcataaaa gtacttatta gtataggtac
     1621 ttttatgata tattatttaa att
//
LOCUS       DAMJWP010000366         1642 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.373, whole genome shotgun sequence.
ACCESSION   DAMJWP010000366 DAMJWP010000000
VERSION     DAMJWP010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.373"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(<1..462)
                     /locus_tag="PC700_003616"
     CDS             complement(<1..462)
                     /locus_tag="PC700_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582856.1"
                     /translation="MEEIDDTILDSMFKLTLNERLLTNNDFVENKIGVVKNLIDCYKT
                     EQNSLMLLKSNFLLALLYGIQGFSEKMKEYILISCKYIDKCLPKDYKFLARFYSQIAI
                     LSLKNEDVNKANLYMDKFNLICDENNFLIEEIIFKSQLIYIKASKCIESSVI"
     gene            complement(593..1012)
                     /locus_tag="PC700_003617"
     CDS             complement(593..1012)
                     /locus_tag="PC700_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3785 domain-containing protein"
                     /protein_id="HDF2582857.1"
                     /translation="MSLYKFIYDDKEYLLKEENCSALINDEENPVQGVSISKIIDILN
                     EAEEVDFDVEYYQEACPLCLEGVKEKKKFFPFLEYHFYIFSKDGEYVISNISVDYKGL
                     SFNKLSRANKVDNSYIVSVIICENCQDYIVQIENCIV"
     gene            1325..1507
                     /locus_tag="PC700_003618"
     CDS             1325..1507
                     /locus_tag="PC700_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582858.1"
                     /translation="MYKDKSDECIHLMTAYIDSISGYYSFIDTQLEDFMVKYGENIVD
                     SNLHSIMMLLCKWGLS"
BASE COUNT          609 a          229 c          141 g          663 t
ORIGIN      
        1 tattacagaa gattctatac acttacttgc cttaatgtaa ataagttgtg atttaaaaat
       61 aatttcttca attagaaaat tattttcatc acaaataaga ttaaatttat ccatatataa
      121 atttgcctta tttacatctt cattttttaa acttaaaata gctatttgtg aataaaatcg
      181 tgctaaaaat ttataatctt tgggtaagca tttatcaata tatttacagg aaatcaaaat
      241 atactctttc attttttcac taaacccttg aataccatat aataaagcta gcaaaaaatt
      301 tgattttaat aacattaaac tattttgttc agttttatag caatcaatta aattctttac
      361 tacaccaatt ttattttcaa caaaatcgtt attagtcaat aatctttcat tcaaggttag
      421 tttaaacatt gaatctaaaa ttgtatcatc tatctcttcc aaatatttac tatatattgt
      481 aactcacctc cgacttacat tatctcataa atttagtaat tttccatact atttttgaaa
      541 aaaattgatg tctcaattta gaaatttctc ttaaattaag acatacttta cactaaacta
      601 tacaattttc tatctgaact atataatcct gacaattttc acaaattatc acacttacaa
      661 tataactatt atcaacttta tttgcccttg atagtttatt aaaagatagt cctttataat
      721 caacagatat attgcttatt acatattctc catctttact gaaaatataa aagtgatatt
      781 ctaaaaaagg aaagaatttt ttcttttctt ttactccttc taaacataat ggacatgctt
      841 cttgataata ttctacatca aaatcaactt cttctgcttc attaagtata tcaattattt
      901 ttgaaatact tactccttga actggatttt cttcatcatt tattaaagca ctacagttct
      961 cctcttttaa taaatattcc ttatcatcat atataaactt gtataaactc atattatatc
     1021 ctccttataa ttattaatct ccaagttatt ctaccactta aataaagtta ataaaagtaa
     1081 ttgtttataa tctttataat taagttatgt atacatatat tcctatacac tatcttaatt
     1141 aatgatttta aaatccaagt ttacactttg aacatccagt ttcccaaaat ctattgtcaa
     1201 aactcacttt agttattaat ataaggatat aaatatataa ctgcatagat gttacacact
     1261 taactattta atatgatacg atttagtatc taaatttata tattgtatat agggggagat
     1321 atatatgtat aaagataaaa gtgatgaatg tatacactta atgactgcat acatcgatag
     1381 tataagtgga tattattcat ttattgacac acaattagaa gattttatgg tgaaatatgg
     1441 tgaaaacatt gttgattcaa atttacacag tataatgatg ttgctttgta aatggggatt
     1501 atcttagcat tttaagaaac ttttttgtat attgtattct atattattta ttcattaata
     1561 aaaaaagatt gacataaact taaaagctat atatagattt ctttaaaaat ctatatatag
     1621 cttttttaat tttttcaaaa tg
//
LOCUS       DAMJWP010000367         1623 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.374, whole genome shotgun sequence.
ACCESSION   DAMJWP010000367 DAMJWP010000000
VERSION     DAMJWP010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.374"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(324..1031)
                     /locus_tag="PC700_003619"
     CDS             complement(324..1031)
                     /locus_tag="PC700_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF2582859.1"
                     /translation="MIFTKSNKYDKDFLMKNMMGPNCIKILEELTSKIKLEKGMRILD
                     LGCGKGISSIFLAKEFDATVFATDLWIEPTENYKRFKEFKLDDKIFPIQAEAHELPYA
                     EGFFDAVISIDSYHYFGNKEGFLDNHISPLVKEGGILAMAMPGLKEDFVDCIPDELIP
                     FWQDNMNFHSITWWNKLWSESESVIVEKCEALNCHDEAWKDWINCDNSYAINDKKMME
                     VENGKYFNTISLIARTK"
BASE COUNT          513 a          282 c          141 g          687 t
ORIGIN      
        1 aatcttcatc aatacctttt ttgctttcat tgttaaaact attttttcta ttagtttatt
       61 tcttataaaa caaacattta gtattaaaat atatatcaca caatagtatt tgcaatttaa
      121 ctttaaaaaa ataaaaatac ccaactgacc taagcaactc gattgggtat aaataatcta
      181 aaaaatcatt tttgtataaa ctctttattt aacttacatt tttatttacc ttctacttta
      241 attcaactta tatttctatg cttcttatac tctcattcaa cttatatttc tacttatctt
      301 atacttttat aaattcatca caattattta gttctcgcta tcaaagaaat tgtattaaaa
      361 tactttccat tctctacttc catcattttt ttgtcattta tagcatatga attatcacaa
      421 tttatccaat ccttccaagc ttcatcatga caatttaaag cttcacattt ctctacaatc
      481 acagactccg attcagacca aagtttattc caccatgtaa ttgaatggaa gttcatatta
      541 tcttgccaaa atggaatgag ttcatctggt atacaatcca caaaatcttc ttttaatcca
      601 ggcattgcca tagcaagtat tcccccctct tttacaagag gtgatatatg attgtctaaa
      661 aatccttctt tgtttccaaa atagtgataa gaatcgatac ttatcaccgc atcaaagaaa
      721 ccttctgcat aaggtagttc atgtgcctct gcctgtattg gaaatatttt atcatccaat
      781 ttaaactctt taaatctttt atagttttca gttggttcta tccataaatc tgttgcaaat
      841 acagtagcat caaattcctt tgctaaaaat atcgaagata tacccttacc acaacctaag
      901 tctaaaatac gcattccctt ttctagtttt atcttacttg ttaattcttc caatattttt
      961 atacaattag gtcccatcat atttttcatg agaaagtcct tatcgtattt attgcttttt
     1021 gtaaatatca ttctatcttt cctttcccta tcagttatta tttattaaat tttatatgtt
     1081 tgatttttta tatgcttctt atattgagca atctcttttt tattcattct ttaacagaca
     1141 attttaatgt gtaattttat ccactctgtg atagtaactt taatgaataa tcttatctaa
     1201 tatgtgataa ttaactttga tgggtaattt tatctactct acaataatca ccttaatgag
     1261 tatcttatct actctattat agttatttta gtgagtaatt ttatctactc cacagtaatc
     1321 gctttaatga ataatcttat ccactctatt atagttattt tagtgagtaa ttttatctac
     1381 tctatagtaa ttgctttaat gagtaatctt atccactcta ttatagctat ttttatgggc
     1441 aattttatct actctataat agtcacttta atgagtaatc ataattttta tatcatttta
     1501 gatacttact ctaataagaa gtcattttct aatacttaat aagttttaat tacttccact
     1561 ttaacctata tactaattaa cttatttaat ttagtgcata tttgacactt taccttttat
     1621 tat
//
LOCUS       DAMJWP010000368         1618 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.375, whole genome shotgun sequence.
ACCESSION   DAMJWP010000368 DAMJWP010000000
VERSION     DAMJWP010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.375"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            152..1555
                     /locus_tag="PC700_003620"
     CDS             152..1555
                     /locus_tag="PC700_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp9"
                     /protein_id="HDF2582860.1"
                     /translation="MKNSKKILAIGLTLFLVMVNTPMVNALTSVEQIKGNDRYETAAK
                     IADKQNYNTAILINSDNSLADGLSASGLAGALNAPILMTKQNQIPNTTMERLNKAKTV
                     YIIGSESTISKNVENQLLSKKKVVQRIFGENRFDTSIKIAEKIKEIKPIDKVIIANGF
                     TGEADAISASPVAARDGVPIILIDGNSVGFDTTGLKSYALGSSEIISDELVKSTNSIR
                     LGGTDRFETNKIVIQEFYKNSKEFYLSKGLQLTDALVASTIAKNAPVVLVENGSNKSI
                     LSGADKLTVLGGINQNVIKQCINQASPNQQGLYYNPNDRAFKERIKGKVYALTKQYRK
                     DNGVRALSVASRLEGLANDWSNLMANKKTLSHTINGKNSYSTFLKYLDWSEIKPGYIA
                     VQGENIIKYKISDKPVYTNRDADDIGNFIFNEWKTNPEEGTNMLHKGYEIMGFGIAIT
                     GDKNLYATHEFYGRYKE"
BASE COUNT          682 a          178 c          312 g          446 t
ORIGIN      
        1 agagaataaa aactttaggt atattgtttg agttgtatgg ataactatgg tatcatatat
       61 aaaatatggt aacaaaattg taacttttga ttaaaaataa gtatgagtac taataaaaaa
      121 tatgaaaagt acattggggg tagaaaataa tatgaagaat agtaaaaaga tattagctat
      181 aggacttaca ctatttttag taatggtaaa tactcctatg gtaaatgcgt tgacatcagt
      241 tgagcaaata aaagggaatg acagatatga gacagcagca aaaatagcag ataagcaaaa
      301 ctacaataca gcaatactaa tcaactcaga taatagctta gcagatggtc taagtgcaag
      361 tggtttagct ggagccttaa atgcgcctat tttaatgaca aagcaaaatc agattccaaa
      421 cacaactatg gaaagattaa acaaagcaaa gactgtgtat ataataggct cagaatcaac
      481 aataagtaaa aatgttgaga atcagttatt atccaaaaag aaggttgtac aaagaatatt
      541 tggtgaaaat agatttgata caagtataaa aatagctgaa aaaatcaaag aaattaagcc
      601 aatagataaa gtaattatag ccaatggatt tacaggagaa gcagatgcga taagtgcatc
      661 accagtagct gctagagatg gagtacctat aatacttata gatggaaata gtgtgggatt
      721 tgacacgaca ggcttaaaga gttatgcgct tggttctagt gaaataatca gcgacgaatt
      781 agttaagagc acaaattcta ttagattggg tggcacagat agatttgaaa ctaataagat
      841 agttatacaa gagttttata aaaatagtaa agaattttat ctaagtaaag gcttgcaatt
      901 aacagacgct ttggtcgcct ctacaatagc aaaaaatgct cctgtagttt tagttgaaaa
      961 tggcagtaac aagagtattt taagtggtgc agacaaattg actgtactag gaggcataaa
     1021 tcagaatgtt atcaagcaat gtataaatca agcttcacca aatcaacaag ggttatatta
     1081 caatccaaat gatagagcat ttaaagagag aataaagggt aaagtatatg ctcttacaaa
     1141 gcagtataga aaagataatg gagtaagagc gttatctgta gcaagcagat tagagggtct
     1201 agcaaatgat tggtctaatt taatggcaaa taagaagact ttatctcata cgataaatgg
     1261 aaaaaattca tattctactt ttttgaaata cttagattgg agtgagatta aacctggata
     1321 tatagcagtt caaggtgaaa atataattaa atataaaatt tcagataaac ctgtatatac
     1381 aaatagagat gcagatgata taggaaactt tatttttaat gagtggaaga caaatccaga
     1441 agaaggaact aatatgttgc ataaagggta tgaaataatg ggatttggta tagctataac
     1501 aggagataag aatctatatg ctacacatga attttatgga agatataaag aataagaaaa
     1561 tatacacaaa attatgcttg taaggaagct atataaataa ttaaattata atggaaaa
//
LOCUS       DAMJWP010000369         1614 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.376, whole genome shotgun sequence.
ACCESSION   DAMJWP010000369 DAMJWP010000000
VERSION     DAMJWP010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.376"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            104..1540
                     /gene="miaB"
                     /locus_tag="PC700_003621"
     CDS             104..1540
                     /gene="miaB"
                     /locus_tag="PC700_003621"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HDF2582861.1"
                     /translation="MSKRKQVTIPIEKIQEQDKYIEQIHRQNEEYFNRTGKRKLVFTQ
                     TFGCQMNEHDSEKLCSMLEEMGYQMSMMVEESDLIIYNTCAVRENAELKVYGNLGQLK
                     HLKGKNPDMKIAVCGCMMQQPHVVEELRKKYKHVDLIFGTHNLYKFPQLLTESINSDK
                     MLVDVWDVDGEVIEGLRSNRKFELKAFVNIMYGCNNFCTYCIVPYTRGRERSRTPEDI
                     INEIKELVANGTKEITLLGQNVDSYGKTLENPVSFSELLRKVNDIEGIERVRFMTSHP
                     KDISDEVIYAIRDCDKVCEFLHLPIQCGSSSLLKKMNRHYTKEYYLEIIEKAKKEVPG
                     IAFSTDLMIGFPGETEEDLLDTLDVVEKVRYDSAFTFIYSKRQGTPAAKMENQIPEDI
                     KHDRFNRVLEAVNRISAEINDGYKDRIVEVLVEGRSKNNENKFAGRTRQNKLVNFEGG
                     NDDLIGKLVMVKITEPRTFSLNGILVNN"
BASE COUNT          691 a          165 c          297 g          461 t
ORIGIN      
        1 attaaaacac aactagaaaa ataataatat ctttaaaaga tttttcttat ttgatataat
       61 taaggttatg tgtaatataa gaaattaact aggaggaata gacgtgagca aaagaaaaca
      121 agtaacaata cctatagaga aaatacaaga acaagataaa tatatagaac aaatacacag
      181 acaaaatgaa gaatacttca acagaactgg aaagagaaaa ctcgtgttta ctcaaacctt
      241 tgggtgtcaa atgaatgaac atgattcaga aaaattatgt tcgatgttag aagagatggg
      301 ataccaaatg tcaatgatgg ttgaagaaag tgatttaata atatataaca cctgtgcagt
      361 tagagaaaat gcagagctaa aagtgtatgg taacttagga caattaaaac atctaaaagg
      421 aaaaaatcca gatatgaaaa tagctgtatg tggatgtatg atgcaacaac cacatgttgt
      481 agaggaatta agaaaaaaat ataaacatgt agaccttata tttggaacac ataatctata
      541 taaatttccg caattactta cagaatcaat aaattctgac aaaatgttag tggatgtatg
      601 ggatgtggat ggagaagtaa tagaaggtct tagaagtaat agaaaatttg aattaaaagc
      661 atttgtaaat ataatgtatg gatgcaataa tttctgcaca tattgtatag tcccatacac
      721 aagaggaaga gaaagaagta gaactcctga agatataatt aacgagataa aagagcttgt
      781 tgcaaatgga acgaaagaaa tcactttact tggacaaaat gtagattctt atggtaagac
      841 acttgaaaat ccagtttcat tctcagaact tttaagaaaa gtgaatgata tagaaggaat
      901 tgaaagggtt cgatttatga catctcatcc aaaagatata tctgatgaag taatatacgc
      961 aattagagat tgtgataagg tgtgtgagtt tttacatcta ccaatacaat gtggaagcag
     1021 tagccttttg aaaaaaatga atagacatta tacaaaggaa tattatttag aaataataga
     1081 aaaagctaaa aaggaagttc caggtattgc tttttctaca gacttaatga ttggtttccc
     1141 aggagaaaca gaagaagatt tattggacac tttagatgtg gttgaaaaag ttagatatga
     1201 ttcagctttt acatttattt attctaaaag acagggaact cctgctgcta aaatggaaaa
     1261 tcaaatacca gaagatataa agcatgatag atttaatagg gtgttagaag ctgtaaatag
     1321 gatttctgca gaaataaatg atggatataa agatagaata gttgaagttc tagttgaagg
     1381 aagaagtaaa aataatgaaa ataagtttgc tggtagaact agacaaaata aactcgtaaa
     1441 tttcgaaggt ggaaatgatg atttaattgg taaattagtt atggtcaaaa ttactgaacc
     1501 tagaactttt tcattaaatg ggatattagt aaataattaa tattgtacaa aataataaaa
     1561 ttatgtaaac taatcgaaat agatgtacta tagaacaaat tatattaaaa attt
//
LOCUS       DAMJWP010000370         1612 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.377, whole genome shotgun sequence.
ACCESSION   DAMJWP010000370 DAMJWP010000000
VERSION     DAMJWP010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.377"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(28..594)
                     /locus_tag="PC700_003622"
     CDS             complement(28..594)
                     /locus_tag="PC700_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF2582862.1"
                     /translation="MDTKKVTFSSICIVINIVLGMVISMINIPLLFLDTVGTVLGAVV
                     LGPFWGALIGGCTNLVLGVISGPTNIPFALVNIVLGLIVGYISKKKGFGYKEAIITGV
                     ILSIVCPLIGTPISVLLFGGLSGSGADLLVGFLVQSGQKIFTAAFFPRILSNIVDKPL
                     SCIMVVYFISKMPKGFIKQYSKNTSSSY"
     gene            760..1281
                     /locus_tag="PC700_003623"
     CDS             760..1281
                     /locus_tag="PC700_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HDF2582863.1"
                     /translation="MKRNREIVVVCHCILNCNSKVEGLSEFKGAQNIAINLINKGYGI
                     IQLPCPEMIMYGINRWGHVKEQFDNLFFREQCRQMLIPHVRQFNEYLKNDYDIKSIIA
                     IDGSPSCGYNKTCSSKRWFGELSGCNELMDKINDIQMVDGKGIFIEELEKLLKEYNLE
                     IPIVGFDELREDK"
BASE COUNT          672 a          188 c          239 g          513 t
ORIGIN      
        1 aataaatctt acataataaa tgatttatta atatgaacta gaagtatttt tactatattg
       61 ttttataaaa cctttaggca ttttacttat aaaatatacc accataatac aagacaaagg
      121 tttgtctact atattactaa gaatccttgg aaagaacgca gcagtaaata tcttctgacc
      181 agactgaact aaaaaaccaa ccaataaatc agcaccactt ccacttaaac caccaaacaa
      241 taaaactgaa ataggagtac caattaaagg acaaacaata gataaaatta ctcctgtaat
      301 aatagcttct ttataaccaa aacctttttt cttagaaata tatccaacaa taagaccaag
      361 aacaatattt acaagagcaa aaggtatatt tgtaggacct gatataacac ctaaaactaa
      421 gtttgtacac ccaccaataa gagcacccca aaaaggacct aatacgacag ccccaagtac
      481 agtaccaact gtatctaaaa ataataatgg tatatttatc atagatataa ccatacctaa
      541 gacaatatta ataactatgc atattgaaga aaatgtgact tttttagtat ccaattataa
      601 tacctcctaa aaaaatatat acatttatca tagtatagat tataaaagat tactaattgt
      661 atatttctat atagaatatt ggaaatatag aacaaataaa taatataatt atcaaaatat
      721 gtagtatcat taaaattaag aataactaag gagattatta tgaagagaaa tagagagatt
      781 gtagtagtat gtcattgtat attaaattgt aattctaaag tagagggatt gtcagaattt
      841 aagggagcac aaaatatagc gataaattta ataaataaag ggtatggaat aattcaacta
      901 ccatgcccag aaatgattat gtatggaata aataggtggg gacatgtaaa agaacagttt
      961 gacaatttgt tttttagaga acaatgtagg caaatgttaa tacctcatgt aaggcagttt
     1021 aatgaatatt taaaaaatga ttatgatata aaaagtatca ttgcaataga tggtagtcca
     1081 agttgtggtt ataataagac atgtagttct aaacgttggt ttggtgagct aagtggttgc
     1141 aatgaattga tggataaaat aaatgatata caaatggtgg atgggaaagg tatttttata
     1201 gaagagctag aaaagctact taaagaatat aatttagaga tacctatagt tggatttgac
     1261 gagcttagag aagataagta aagatataaa tatttattgt ctcattatga aacaccatta
     1321 tatttggcag aaaaatatga ctgatggaca gtacctgaat catttttaag tggagtaatt
     1381 agtacttata aagatgtatc tactagctga aagaaaatgt agagaggtaa taaatgaatt
     1441 gataatatat ataaaggagt gtctaaaagt gaacttttta gtttatgaga cactcttttt
     1501 ttatattaaa tcaagtatat taaatcaaat atattttaat cattttagca atgaaaaata
     1561 aatttatcta tattttgaga caacatcttt tttagtttta tttatttgtt gt
//
LOCUS       DAMJWP010000371         1609 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.378, whole genome shotgun sequence.
ACCESSION   DAMJWP010000371 DAMJWP010000000
VERSION     DAMJWP010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.378"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            262..1533
                     /gene="serS"
                     /locus_tag="PC700_003624"
     CDS             262..1533
                     /gene="serS"
                     /locus_tag="PC700_003624"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDF2582864.1"
                     /translation="MLDIKRIRENLDCIKKAMERRGEKDFNLDEVVKLDDERRKILQE
                     VEVMKHKLNTASKNIPNLIKEGKDVENEKIKLKELSDKIKVIDQNLKEVEDKMEYLLM
                     RIPNVPHPEVPQGETDEDNVEVRTWGKTTTFDFESKAHWEIGTELGILDFETASKITG
                     SRFTLYKGLGARLERALLNFYLDTNTKVNGYTEVIPPFMANRNSFLGTGQLPKFEEDM
                     FKIEGLDYFMIPTSEVPLTNIHANEILKFEQLPINYTAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVEMVKIVAPEESYNELEKLTNNAETMLQLLNLPYRVVKICTGDLGFTASFK
                     YDVEVWMPSYNRYVEISSCSNCEDFQARRAGIRFKRDKDSKAEYVHTLNGSGLAIGRS
                     VAAILENYQQEDGSVVVPEVLRPYMGVSVIK"
BASE COUNT          674 a          171 c          292 g          472 t
ORIGIN      
        1 aaagtcaaaa tagataattg attagcaaaa tagatataat tataatgaag ttaaatctag
       61 caatattctc ctaaaattaa atttcattta tttaattaaa tataatttga caatggacaa
      121 aaaagtgagt aaaatataaa taaaataaaa ataacaggtt cggataaaga aaaaggtttc
      181 ttaacctcaa gcataaagta tattatctaa gttatttgag attaagagat aaatccaatc
      241 ttaggtactg aggaggaaaa aatgcttgat ataaaaagaa taagagaaaa tttagattgt
      301 ataaaaaaag caatggaaag aaggggcgaa aaagacttta accttgatga agtagttaag
      361 ttagatgatg aaagaagaaa gattcttcaa gaagttgaag ttatgaaaca taaattaaat
      421 acagcatcta aaaatatacc aaatcttata aaagaaggta aagatgtaga aaatgaaaag
      481 attaaactaa aagagttatc tgataaaatt aaggtaatag accaaaatct taaagaagta
      541 gaagataaga tggaatacct attaatgaga ataccaaatg taccacatcc agaagtacca
      601 caaggtgaaa ctgatgaaga taatgttgag gtaagaactt ggggaaagac tactaccttt
      661 gattttgaat ctaaggctca ttgggaaatt ggaacagaat taggtatact tgattttgaa
      721 acagcgagta aaataactgg ttcaagattc actctttaca aaggattggg tgctagatta
      781 gagagagcat tattaaattt ttacttagac acgaatacaa aagtaaatgg atatacagaa
      841 gtcattccac catttatggc aaatagaaat agttttttag gtacaggaca gttacctaag
      901 tttgaagaag atatgtttaa aatagaaggt ctggactatt ttatgattcc aacatcagaa
      961 gttcctttaa ctaatataca tgctaatgaa atacttaaat ttgaacaatt accaataaat
     1021 tatacagctt atactccatg ttttaggtca gaagctggtt ctgctggaag agatacaaga
     1081 ggtttagtaa gacagcatca gtttaacaaa gtagaaatgg ttaaaatagt tgcaccagaa
     1141 gaatcttaca atgagttaga aaagcttact aacaatgcgg aaactatgct acaattattg
     1201 aatctgcctt atagagtagt aaagatatgt acaggtgatt taggatttac ggcttctttt
     1261 aaatatgatg tagaagtttg gatgccaagt tacaataggt atgttgagat ttcatcttgt
     1321 tcaaactgcg aagatttcca agctagaaga gcaggaataa gatttaaaag agataaagat
     1381 tctaaagctg aatatgtaca cacattaaat ggctctgggt tagctattgg aagaagtgtt
     1441 gcagctatat tagaaaatta tcaacaagaa gatggttctg tggttgttcc tgaagtttta
     1501 agaccatata tgggagtttc tgttattaaa taagagttaa attatgtatt tttaagcaat
     1561 acaataatat agaagtaata aaaaataatt aatataaaag tgatacaat
//
LOCUS       DAMJWP010000372         1606 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.379, whole genome shotgun sequence.
ACCESSION   DAMJWP010000372 DAMJWP010000000
VERSION     DAMJWP010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.379"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(81..1562)
                     /gene="cls"
                     /locus_tag="PC700_003625"
     CDS             complement(81..1562)
                     /gene="cls"
                     /locus_tag="PC700_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDF2582865.1"
                     /translation="MFDISFFEMSIYEIAVTTIYIINFMVILNLIFREKRNINTTLTW
                     LLILVLIPALGFILYIAFGRNISKNNMFRLKEKDDKIIKSNILDTQVKLQSTSEIDSD
                     IHQHKDMIYALANSNNAHYTNNNDVWIYAESSQFFNSLLEELKKAKKYINIQFYIFKD
                     DKIGTEIIDILIDKAKEGVEVRLLFDAVGGRTLKNSTLSRLKESGVKVGSFFPSFLKI
                     VNFNLNYRNHRKIVVIDGKVGFVGGYNVGDEYLGRNPKFGLWRDTHTKLTGDCVIDLN
                     MRFILDWRYTTKEDLDLEKYFIEEDTNSNHPSENIGIQIVSSGPDISELDEIKYGYLK
                     MIQKARKYIYIQSPYLILDSTFIDTLKIACLSGVDVRVMIPSKPDHPFVYWASYSYAG
                     ELLKFGAKIYTYGQNAFLHAKTIVIDDSICSIGTANMDIRSFELNFEVNAFMYSSKKA
                     LEQRIIFENDILNSKEITLDIYNSRSTYIKIKESISRLLSAVL"
BASE COUNT          573 a          242 c          189 g          602 t
ORIGIN      
        1 aattcaataa aaaaaaaata atacatataa aaaactttaa aaaataaacc aaaatgtaaa
       61 atttttataa aataaaagat ttataaaact gctgataata gtcttgaaat tgattccttg
      121 atttttatat aagtagacct agaattatat atatctaatg ttatttcctt agaatttaaa
      181 atatcatttt caaatataat tctttgttcc aatgcttttt ttgaagagta cataaatgca
      241 ttaacttcga aattaagctc aaaacttctg atatccatat tagctgttcc tatagagcat
      301 atagagtcat ctataactat tgtcttagca tgtaaaaatg cattttgacc ataagtgtag
      361 attttagctc caaactttaa aagttctcca gcatatgaat atgatgccca gtatacaaag
      421 ggatggtcag gtttagaagg aatcataact ctcacatcaa caccagataa acaagctatt
      481 tttaaagtat ctataaaagt tgagtcaagt attaaataag gactctgtat gtatatatac
      541 tttctagctt tctgaatcat tttaaggtaa ccatatttaa tctcatcaag ttctgatata
      601 tctggaccac ttgatactat ctgaatccca atattttcag aaggatgatt agaatttgta
      661 tcttcttcta taaagtactt ttctaaatct aaatcttctt tagttgtata tctccaatca
      721 aggatgaatc tcatatttag gtcaattacg caatcaccag taagcttagt atgtgtatct
      781 ctccatagac caaattttgg atttctacct aaatattcat caccaacatt atatccacct
      841 acaaatccta ccttaccatc aatgacaaca atttttcggt gatttctata gtttaagtta
      901 aagtttacaa ttttcaagaa tgatgggaag aagcttccaa ctttaacacc actttcttta
      961 agtctggaaa gtgtactatt ttttagtgtt ctaccaccaa ctgcatcaaa taaaagtctt
     1021 acttcaacac cttctttagc tttgtctata agaatgtcta ttatttcagt tccgattttg
     1081 tcatctttga aaatgtaaaa ttgtatgttt atatattttt tagctttttt aagttcttca
     1141 agaagactgt taaaaaattg actagattca gcataaatcc acacatcgtt attattggta
     1201 taatgagcat tatttgaatt agccaatgca taaatcatat ccttatgttg atgtatatct
     1261 gaatcaattt cagatgtaga ttggagttta acctgagtat ctagtatatt acttttgatt
     1321 atcttatcat ccttttcttt caatctaaac atattgtttt ttgaaatatt tctgccaaaa
     1381 gctatataaa gtatgaatcc aagagcagga atcaatacta atataagaag ccatgttaaa
     1441 gttgtattta tgtttctttt ttctctaaag ataaggttta gtataaccat gaaattaatt
     1501 atatatattg tagtaactgc tatttcatat attgacattt cgaaaaatga tatatcaaac
     1561 ataaaattcc ctccttcaaa tatttattaa aataagtaat tatata
//
LOCUS       DAMJWP010000373         1597 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.380, whole genome shotgun sequence.
ACCESSION   DAMJWP010000373 DAMJWP010000000
VERSION     DAMJWP010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.380"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            89..829
                     /locus_tag="PC700_003626"
     CDS             89..829
                     /locus_tag="PC700_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HDF2582866.1"
                     /translation="MKNFRDLGGNKTEDGRTVKKGLFYRSAKLSNLSENDIKILKELN
                     IKYIFDYRSDEEARKHPSTIISNIKNIRIPAMRELEESGGSFGSIEDMIDGLFEKDGA
                     FNMLNNSYYNLPINNPSYKKLVELIRDYSNLPILNHCTAGKDRTGVGSAIILMILGVS
                     RENIMKDYLKSNDFADKEIERFIEYKPKFKDIPKENLKYIFGVNEEYMKTAFRRIDEE
                     YISVEAYLYGEFNLNKEEIRKLRNQYLE"
     gene            841..>1597
                     /locus_tag="PC700_003627"
     CDS             841..>1597
                     /locus_tag="PC700_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDF2582867.1"
                     /translation="MSLNITARQINIIKILLNSKDTISGIALSQEIGCSSKTIQNEIK
                     DINKQLRNGKILSIRGVGYKLEGSFDEVNLNSNLYDDVDRVEYIIRKILTLSNEEKNT
                     IKLEDLADSMYVSVSTVKNDLKEVKRILEKYSISVKSKHKQGICVLESEDKILNCIVD
                     LSNKRDNQLSLNDFLNDDIKERKLLIKKLLLDILNENGLVLTDIEFKSVLNNILVMLS
                     RHKYNEKEFIENYIENYRSKRSSILENEQNKEII"
BASE COUNT          728 a          105 c          275 g          489 t
ORIGIN      
        1 tattttaaat aaagaactta aaattgtgat aaaattaatt tatagatatt aaaaaaagta
       61 taatatatgg tttttaagag gtgctaagat gaaaaatttt agagatttag gtgggaacaa
      121 aactgaggat ggcagaactg taaagaaggg attattttat aggtctgcaa aattatcaaa
      181 tctaagtgaa aatgatataa agattttaaa agagttaaat attaaatata tatttgatta
      241 tagaagtgat gaagaagctc gtaaacatcc atcaacaata atatcaaata taaaaaatat
      301 aagaatacca gcaatgagag agctagaaga atcaggagga agttttggtt ctattgaaga
      361 tatgattgat ggattgtttg aaaaagatgg tgcatttaat atgttaaata atagttacta
      421 taatttgcct ataaataatc cgtcatataa aaaattagta gaacttataa gagactattc
      481 taatttacca atattaaatc attgtacagc tggtaaggat agaactggtg tggggagtgc
      541 tataattctt atgattttag gggtatcgag agagaatata atgaaagatt atctaaaaag
      601 taatgatttt gcagataaag agatagaaag atttatagaa tataagccta aatttaagga
      661 cattccaaaa gaaaatttaa aatatatatt tggtgtaaat gaagagtata tgaaaactgc
      721 atttagaaga attgatgaag aatacattag tgtagaagca tatttatatg gagaatttaa
      781 tttaaataaa gaagaaataa gaaaattaag aaatcaatat ctagagtaaa aggtgagttt
      841 atgagtttaa atataacagc aagacagata aatataataa aaatattgtt aaattcaaaa
      901 gatacaatta gtggtattgc cttaagtcaa gagataggat gttcttcaaa aactatacaa
      961 aatgaaataa aggatattaa taaacaactt agaaatggta aaattttgtc aataagagga
     1021 gttggttata aactagaagg aagttttgat gaggtaaatc taaatagcaa tctctatgat
     1081 gatgtagata gagtagaata tataatcaga aaaatattaa ccttaagtaa tgaagaaaaa
     1141 aatactataa aacttgaaga tttagccgat tctatgtatg taagtgtttc aacagtaaaa
     1201 aatgatttaa aagaagtaaa aagaatattg gaaaagtatt caatatcagt taaatctaag
     1261 cataaacaag gtatatgtgt tttggaaagt gaagataaga tattaaattg tatagttgac
     1321 ttaagcaata agagagataa tcaattgagt ttaaatgatt ttttaaatga cgatataaag
     1381 gaaagaaaac tattaataaa aaaattatta ttagacatat taaatgaaaa tggattagta
     1441 ttaactgata tagaatttaa gagtgtgtta aataatatat tagttatgtt aagcagacat
     1501 aagtataatg aaaaagaatt tatagagaat tatatagaaa attataggag caaaagaagc
     1561 tcaattttag aaaatgaaca aaacaaagaa attataa
//
LOCUS       DAMJWP010000374         1517 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.381, whole genome shotgun sequence.
ACCESSION   DAMJWP010000374 DAMJWP010000000
VERSION     DAMJWP010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.381"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     misc_binding    476..739
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            890..1441
                     /locus_tag="PC700_003628"
     CDS             890..1441
                     /locus_tag="PC700_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan transporter"
                     /protein_id="HDF2582868.1"
                     /translation="MKTNTKKLTLNAILLAMGLLIHQITPAIGLPMKPDVPLAMLFVI
                     LVLNRDDYKTCLIAGIVTGIFTALTSSFPGGQIPNVIDKTLTANIVFLLMNISYNMPF
                     IKKLAKKTQDTVVVAIIMPIGTLVSGTIFLLAAQAIVGLPGASFTALFLAVVLPAVLI
                     NLVAGIFLFKIVSLSIRRVSYQA"
BASE COUNT          624 a          151 c          234 g          508 t
ORIGIN      
        1 gctaatattt attaaaagaa agaagaaaaa attttactaa aaataattaa aagaatatca
       61 tttttctaat cattggtatc tatttaataa tatgatgtaa ttaaaatgga gtaatattct
      121 gtttcaaagg aggcatataa atacaggaat gagtattaaa tgatggttaa ttaagtaaat
      181 ttattgataa atgtacttaa ttaactttga agaatttaat tcctcatgaa tcaaaatata
      241 aaattacatc aacagttatt cacaaaatta atataagaag aattagagcc aaagagcaga
      301 ctattagacc attaaagtaa ttattcatag aattttgtat atgaaaagaa atgtatagta
      361 taatttactt tttttataaa aaatagatta caaaatatat tttttataaa aaggtattga
      421 cattttttag aaaaaagaat atcattattt taacaacaaa attcaataca ataaattaaa
      481 tcctatgaca aggaagagta gttaaattta ggttcagtat agagagctga ggatggtgga
      541 atcttagcaa gaaaaaattt gatgaatggc ccttagagga ataaattgaa atcatagttt
      601 tgagagtaga ctttatcgtc agtcgcacgt tatagcgaat aaggtatgta agtacctgat
      661 aatgagatgt atgaaaattg aattttatat taatttttat ataatttagg tggcaacgcg
      721 gataatctcc gtcctattag tttaggacgg agttttttat tttactgaaa atttaaaaga
      781 tagaaggtgg tgagaaagat gttatgaatt tcaaaagcta aagatgatga tatagtaggt
      841 tggcaaaaaa ataaattgaa aattaaaaaa ataggaggaa gtacaaaaaa tgaaaacaaa
      901 tacaaaaaaa ttaactttaa atgcaatact tttagcgatg ggattattaa tacatcaaat
      961 cacaccagct ataggattac caatgaagcc agatgtacca ctagcaatgt tatttgtaat
     1021 attggtttta aatagagatg attataaaac ttgtttgata gcgggtatag taacaggaat
     1081 atttacagca cttacatcaa gttttccggg aggtcaaatt cctaatgtaa tagataaaac
     1141 tcttacagca aatatagtat ttttattaat gaatattagt tataatatgc cttttataaa
     1201 aaaattagct aagaaaacac aagatactgt agtagttgca ataataatgc caataggaac
     1261 tcttgtaagt ggaactatct ttttattagc agctcaagct atagttggtc ttccaggagc
     1321 ttcatttaca gctttattct tagcagtagt attaccagct gttttaataa atctagttgc
     1381 aggaatattt ttattcaaga ttgttagtct atcaataaga agagttagtt atcaagctta
     1441 actacgaaat aatattttac tttataaagc attataataa ctaaataaca taagaataaa
     1501 gtagattata actattt
//
LOCUS       DAMJWP010000375         1474 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.382, whole genome shotgun sequence.
ACCESSION   DAMJWP010000375 DAMJWP010000000
VERSION     DAMJWP010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.382"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..1050
                     /locus_tag="PC700_003629"
     CDS             <1..1050
                     /locus_tag="PC700_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDF2582869.1"
                     /translation="IQYGFLLFLICITTYLVLTTLDIKLIPKIIRLVNKTYIFLGILT
                     IIVYMIIESIVTHLIINAVQKTKVKFIGIKMATMGFYYNLVTPFASGSQPMQIYALTK
                     YDVSLSKSVAIVTNKTVVFQSTVTVFCGILILLNKSLLMEQVHSVKVLITFGMIMNVS
                     MLLGGLLIVFSPKQVKMIAEVLINFLSKFKTFKFLDSKRDRINHYIDDYNYSIKIFIK
                     NVRILVLSILLTVLQLIAYFSIVYCVYKASNLRGVSYVHIMTLQVFLYMAISPIPTPG
                     NVGANEVTFFTMFSNIIPKELLGYSVLLYSGFVYYFILIVSGLFTFGTHYTLNSWKKE
                     DKVNSNLDKVIEKLN"
     gene            1199..1360
                     /locus_tag="PC700_003630"
     CDS             1199..1360
                     /locus_tag="PC700_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HDF2582870.1"
                     /translation="MKQDKQNEKQSEISKIKQSNDNKLEIAQELHVSKNIGNMLGYRI
                     DKKKMNRPS"
BASE COUNT          593 a          131 c          215 g          535 t
ORIGIN      
        1 attcaatatg gatttttatt atttttgata tgtattacta cttatttagt attaactact
       61 ttagatataa aactaatacc taaaattata aggctagtaa ataagactta tatatttctt
      121 ggcatattga caattatagt atatatgata attgaatcta ttgtaactca tttgataata
      181 aatgcagttc aaaaaacaaa agtaaaattt attggtatta aaatggctac aatgggattt
      241 tactataact tagttactcc atttgcatct gggagtcaac caatgcaaat atatgcactt
      301 acaaaatatg atgtaagcct tagtaaatca gtggctatag ttactaataa aactgtagta
      361 tttcaaagta cagttactgt attttgtgga attttaattt tgctaaataa gtcattactg
      421 atggagcaag tacattctgt gaaagtcttg ataacttttg gaatgattat gaatgtaagt
      481 atgttgttag gtggactatt aatagtattt agtccaaaac aagtaaaaat gatagcagaa
      541 gttttgatta attttttatc taaatttaaa acatttaaat ttttagattc aaaacgtgat
      601 agaataaatc attatattga tgattataat tattctataa aaatatttat taaaaatgta
      661 agaatcttag ttttaagtat attacttact gtgttacaat taatcgcata tttcagtata
      721 gtttattgtg tgtataaagc ctcaaattta agaggagttt catatgtaca tataatgaca
      781 ttacaggtct ttttatatat ggctatatca ccaattccaa caccaggaaa tgttggagct
      841 aatgaggtta cattttttac tatgttctcc aatataattc caaaagaatt attggggtac
      901 tctgtactct tatatagtgg gtttgtatac tattttatat taatagtttc aggtttattt
      961 acatttggaa cgcattatac attaaattct tggaaaaagg aagataaagt aaattctaat
     1021 ttagataagg ttattgaaaa attaaattaa taaaataatt agggaaattc tatatataaa
     1081 tatggtgttg agtaagctat attatattga gatatttccc ttttttgatt aaaattatag
     1141 aaatttgaaa taataagtaa taaacagaaa aagtgatatt gttttgaaag gagaaactat
     1201 gaaacaagat aaacaaaatg aaaaacaaag tgagatttct aagataaaac aaagcaatga
     1261 taataaactt gaaatagctc aagaattaca tgtaagtaaa aatataggga atatgcttgg
     1321 atatagaata gataagaaaa aaatgaatag accaagttaa tgaaaatgtt gaaaatggta
     1381 cattttaaaa aatatatata atgaaatgtt ttattgttaa atttaaactt attttaatgg
     1441 aggaaaaagt cgaaaaatat ctaaaaagat atgg
//
LOCUS       DAMJWP010000376         1456 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.383, whole genome shotgun sequence.
ACCESSION   DAMJWP010000376 DAMJWP010000000
VERSION     DAMJWP010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.383"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            78..1187
                     /gene="ugpC"
                     /locus_tag="PC700_003631"
     CDS             78..1187
                     /gene="ugpC"
                     /locus_tag="PC700_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HDF2582871.1"
                     /translation="MSEVILKNISKLYSNGFNAVKNINIDIKDKEFIVLVGPSGCGKS
                     TTLRMIAGLEEISEGELYIGDKLVNDIEPKDRDIAMVFQNYALYPHLSVYENMAFALK
                     LRKLPKDEIDKKVKEAAKILDLLPLLNKKPKTLSGGQRQRVALGRAIVRNPKVFLMDE
                     PLSNLDAKLRTAMRTEITKLHQQLGTTFIYVTHDQVEAMTMADRIVVMKDGVVQQIAT
                     PQDVYDYPANIFVAGFIGAPQMNFIDVILIEENDKIYAQNEHIKLKLNKEKHYDLIKD
                     NYINNEVVIGIRPEDIHVEDTFIKSNPDTCFKSRIEIKELMGAETYAHLKLGENTITI
                     RFDSKNRINVGDDLTLSVDNSRVHIFDKETTLAIR"
     gene            1217..>1456
                     /locus_tag="PC700_003632"
     CDS             1217..>1456
                     /locus_tag="PC700_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HDF2582872.1"
                     /translation="MEELKDFLERQLNKKINIYPYENQKLDASSHLVTFNNAIYVIDF
                     LDKNNLDNLKGNFYLIYQSHIDFEYLKNIFYNLFED"
BASE COUNT          598 a          184 c          217 g          457 t
ORIGIN      
        1 ttagtcattt tacacattta atataataaa aaaatttgat actatatcat taaaatttaa
       61 atatcaagga gaatattatg tcagaagtaa ttttaaaaaa tatatcaaaa ctttactcaa
      121 atggatttaa cgcagtaaaa aatataaata tagacattaa agataaagaa tttatcgttt
      181 tagtaggacc atctggttgt ggtaaatcga ctacacttag aatgattgct ggtcttgaag
      241 aaatctctga aggagaacta tacattggag ataaacttgt caatgatata gaaccaaaag
      301 atagagatat agctatggtt tttcaaaact atgctttata tccccactta tctgtttacg
      361 aaaacatggc atttgcactt aaacttagaa aattgcctaa agatgaaata gacaaaaaag
      421 ttaaagaagc tgctaaaata ttagacttac tacctctttt aaataaaaaa cctaaaacat
      481 tatctggagg acaaagacaa agggttgcct taggacgtgc aatagttaga aatcctaagg
      541 tattcttaat ggatgaacct ctatccaact tagatgcaaa acttagaact gctatgagaa
      601 cagagataac taagctacac caacagcttg gaactacatt tatctatgta acacacgacc
      661 aagttgaagc tatgactatg gctgatagaa tcgtagtaat gaaggatgga gtagttcagc
      721 agattgcaac tccacaagat gtatacgatt atccagctaa tatatttgtt gcaggattta
      781 ttggagcacc acagatgaat tttattgatg taatattaat agaagaaaat gataaaattt
      841 atgcacaaaa tgaacatatt aagcttaaat tgaataaaga aaaacactat gatttaataa
      901 aagacaatta tattaataat gaagttgtta taggtatcag accagaagat attcatgtag
      961 aagatacttt tattaaatct aatccagata cttgttttaa atcaagaatt gaaataaaag
     1021 aacttatggg agcagaaact tatgctcact taaaactagg cgaaaatacg ataacaatta
     1081 gatttgacag taaaaacaga attaatgttg gtgatgactt aacactttca gttgacaata
     1141 gtagagtaca catatttgac aaagaaacta ctttagctat aagataagaa tataatctta
     1201 tttttaagga gactttatgg aagagttaaa agatttttta gaaagacagc ttaataaaaa
     1261 aataaatatc tatccctatg aaaaccaaaa gttagatgct agtagccatt tagttacttt
     1321 caataatgct atttatgtta ttgattttct agataaaaat aatcttgata atttaaaagg
     1381 caacttttat ttaatttacc aatctcatat agactttgaa tacctaaaaa atatttttta
     1441 taatttattt gaagat
//
LOCUS       DAMJWP010000377         1440 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.384, whole genome shotgun sequence.
ACCESSION   DAMJWP010000377 DAMJWP010000000
VERSION     DAMJWP010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.384"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            52..1290
                     /locus_tag="PC700_003633"
     CDS             52..1290
                     /locus_tag="PC700_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HDF2582873.1"
                     /translation="MNKGFYKLNSVVLNFDIKNDVNKIRFNHYIMRKENIQRLQGTLP
                     NGQFYMTVRRAATDLQLSISTISRLVKEFLDLGIIRLVSKGVKGNRSVYSYVSIEFNG
                     SLFDDSKYMELDCVCNDEFIDNSGIEFDCDSKDNSFNGDINVETVHCINEYFSDDDVE
                     HNYNNFHNKDTIKDKAISNISTIKKYIDESFDTNKSENSNLQQESINFNFNDTCNEID
                     KRTNNSIENNQNKRPMRLSDISVLSDKGMTNKKNYFNHNHGIQKRNKSATKKKESLNK
                     FNKKNIQQSIINMLNKKSGKNFKIDSPISIKLINERLKEGYVLEDFYKVIEVKVAKWK
                     NTIMEMYIRPETLFSYKFESYVNENISMKSQSNVHPCKEGNSNDSSNYIHSDYTTYDG
                     SVRERKKYENVRYWNPICEI"
BASE COUNT          612 a          134 c          251 g          443 t
ORIGIN      
        1 aaatacttat ctagttacta gcaattatta gtagaaatgg gggaaataga gatgaataag
       61 ggattttaca aattaaattc agtggtattg aactttgata ttaagaatga tgttaacaag
      121 ataagattta atcattatat aatgagaaaa gaaaacatac aaagacttca aggaactctg
      181 ccaaacggac aattttatat gacagtgaga agagctgcta cagatttaca actttctatt
      241 tctactataa gtaggcttgt aaaagagttt ttggatttag ggataataag attagtgtct
      301 aagggtgtaa aaggtaatcg ttctgtatat agttacgtaa gtatagagtt taatggaagt
      361 ttatttgatg atagcaaata tatggaactt gactgtgtat gtaatgatga atttattgat
      421 aatagtggca tagagtttga ttgtgatagc aaagataatt cttttaatgg agatataaat
      481 gtagaaactg tgcattgcat taatgagtat ttctctgatg atgatgtaga gcataattat
      541 aacaatttcc ataataaaga tactataaag gataaagcca tttctaatat tagcactatc
      601 aaaaaatata ttgatgaatc ttttgataca aataaatcag aaaattctaa cttacaacag
      661 gagtcaataa atttcaactt taatgataca tgcaatgaaa tagataaaag aacgaataat
      721 agtatagaaa ataatcaaaa taaaaggcct atgagattga gtgacatcag tgttttaagt
      781 gataaaggta tgacaaataa aaagaattac tttaatcata atcatggtat acaaaaaagg
      841 aataaatctg caacaaagaa aaaagaatcc ttaaataaat ttaataaaaa aaatatacag
      901 caaagcatta taaatatgct aaataaaaaa tcaggtaaga attttaaaat cgattctcca
      961 ataagcataa aacttatcaa tgaaaggcta aaggagggct atgtcttaga agatttctat
     1021 aaagttattg aggtaaaagt ggctaagtgg aaaaatacca taatggaaat gtatataaga
     1081 ccagaaacct tgtttagcta taagtttgaa tcctatgtaa atgaaaacat atctatgaaa
     1141 agtcaaagta atgttcatcc atgtaaagaa ggcaatagta atgatagcag taattatata
     1201 cattctgatt atacaacata tgatggcagt gtgcgtgaga gaaaaaaata tgagaatgta
     1261 agatactgga atcctatatg tgaaatttaa ttagtttaag aatgtattta tgataaataa
     1321 gatattaata gagaatagtg agctatagca aatgatatat caatagttgg tagtaagtta
     1381 tattaagcaa gatattacta tcaggtagtg aattatagtg aacgatatat caataattgg
//
LOCUS       DAMJWP010000378         1433 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.385, whole genome shotgun sequence.
ACCESSION   DAMJWP010000378 DAMJWP010000000
VERSION     DAMJWP010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.385"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..>1433
                     /locus_tag="PC700_003634"
     CDS             <1..>1433
                     /locus_tag="PC700_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell wall-binding protein Cwp28"
                     /protein_id="HDF2582874.1"
                     /translation="KFYKRILTLTLSISFVFANIQLVDAISSVEKIQGKNKYEIAGKI
                     ADKNAYKTAILINTSNSIADGLSASGLAGALNAPILLTEKNTIPTETSARLKNVSKVY
                     IIGGTYSISTSVENSLKSKKMKVVRIKGNDRIKTSYNVAKEINSIKKVNTVMLTNAYK
                     GEADAISIASVAARDKAPIILTNGQSIPFSTSGLKSYVIGGTASMSTTLVNNTKSTRL
                     GGSTRFETNKAIINKFYKDAREFYIAGAYELTNALVGSSLSKHGPIVLVNDGSNKSIL
                     KNAKKITSIGYIDSNIVQQCLNITNGIGDINTGVVKNVKPTTKTIKDGMYKVGKDISA
                     GEYLITSNSGSYASYYEVTSDSTGNADSILSNDIFSGTRYITLKNGQYIKIEDSTMTL
                     AKYAKAQKAKNGKFGNGMYKIGLEIPAGEYIIMSNSSDAYYEVRNDSLGNAEGIVTND
                     TFSGRRYITVEEGQYLILNDCYLIENE"
BASE COUNT          591 a          157 c          250 g          435 t
ORIGIN      
        1 gaaattctat aaaagaatat taacattgac tttaagtatt tcttttgtat ttgcaaatat
       61 tcaattagta gatgcaatat catctgtaga aaaaatacaa ggaaaaaata agtatgaaat
      121 agcaggcaaa atagcagata aaaatgcata taaaactgct atattaatta atactagtaa
      181 ctctattgca gatggattaa gtgcaagtgg actagcagga gctttaaatg cccctatact
      241 attaactgaa aaaaatacta taccaactga gacatctgca agattaaaaa atgtatcaaa
      301 agtttatata attggaggaa catattctat tagtacatct gtcgaaaatt cattaaaatc
      361 taagaaaatg aaagttgtaa gaattaaggg aaatgacaga ataaaaacaa gttataatgt
      421 tgcaaaagaa ataaattcaa taaaaaaagt caatactgtt atgctaacca atgcatataa
      481 aggagaagca gatgctataa gtattgcatc tgtagcagca agagataaag caccaataat
      541 tcttactaat ggacaaagta tacctttttc aactagtggt ttaaagtctt atgtaatagg
      601 aggaacagct tcaatgagta ccactttagt aaacaatact aaatctacta gactaggagg
      661 gagtactcgt tttgaaacta ataaagcaat aataaataag ttttataagg atgctagaga
      721 gttttatata gctggagctt atgaattaac aaatgcactt gtaggatctt ctttatcaaa
      781 acatggtcca atagttttag ttaatgatgg tagcaataaa agcattttaa aaaatgctaa
      841 gaagataact tctataggat atatagattc aaatatagta caacagtgtt taaatataac
      901 aaatggaatt ggagatatta atactggggt tgtaaaaaat gttaaaccta ctacaaagac
      961 tataaaagat ggaatgtata aggttggaaa agatatatca gcaggtgaat acttaattac
     1021 ttcaaactca ggaagttatg cttcttatta tgaagttaca agtgatagta caggtaatgc
     1081 tgatagtata ctttcaaatg atatttttag tgggactaga tatatcacct taaagaatgg
     1141 tcaatatatt aaaattgagg attcaacaat gacattagca aagtatgcta aagcccagaa
     1201 ggccaagaat ggtaagtttg gaaatggtat gtataaaatt ggattagaaa ttcctgctgg
     1261 agaatatatt ataatgtcaa attcttctga tgcatattat gaagttagaa atgatagctt
     1321 aggtaatgca gagggaatag ttactaatga tactttttca ggaagaagat atataacggt
     1381 tgaggaaggt caatatttaa tattaaatga ttgttattta attgaaaatg aat
//
LOCUS       DAMJWP010000379         1395 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.386, whole genome shotgun sequence.
ACCESSION   DAMJWP010000379 DAMJWP010000000
VERSION     DAMJWP010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.386"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            1..612
                     /locus_tag="PC700_003635"
     CDS             1..612
                     /locus_tag="PC700_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HDF2582875.1"
                     /translation="MINDIKNKFKNYKPYINGYKNMKRASVLIPIIEINNTHYILFEV
                     RSKNLKHQPSEISFPGGKIESGETPYEAVIRETCEELGTFSDNIEVISSLDLLITPVK
                     FIIHPYLGYIKNINNLNINKDEVDHTFLVPIKYLLENPPNTYSNTVKVFPDENLPYGS
                     IPNQKDYKFESGSYPILFYKYKDYVIWGMTAKILENFLNFLSE"
     gene            910..1248
                     /locus_tag="PC700_003636"
     CDS             910..1248
                     /locus_tag="PC700_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HDF2582876.1"
                     /translation="MFAVGFVVIIALAFFILSVLLFNGKASMLLAGYNSMSKKEKARY
                     DKKKLCRSSSIVTFIVSIMLFIMAFLGYRIESGQMDEKKMVPFSIIFIIVILSSVFFN
                     ILYSNKKCKK"
BASE COUNT          549 a          157 c          184 g          505 t
ORIGIN      
        1 atgataaacg atattaaaaa taaattcaaa aattacaaac catatataaa tggatacaag
       61 aatatgaaga gagcatctgt tttaattcca ataatagaaa taaataacac acactatata
      121 ttatttgaag ttcgctcaaa aaatttgaag catcaaccta gcgaaatttc ttttccaggt
      181 ggtaaaatag aatcaggtga gactccatat gaagctgtta ttagagaaac ttgtgaggaa
      241 cttggaactt ttagtgacaa tatagaagta atatcaagtc ttgacctcct tataacacca
      301 gtaaagttta tcatacatcc atacttagga tatataaaaa atattaataa tttaaatata
      361 aacaaggatg aagttgacca tacttttttg gttcctataa aatatctttt agagaatcct
      421 ccaaatactt atagtaatac tgtaaaagtt tttccagatg aaaatctacc atatggttca
      481 attccaaacc aaaaagatta taaatttgaa agtggaagtt atcctattct gttttataaa
      541 tataaagatt atgtaatttg gggaatgaca gcaaaaatac tagaaaactt tttaaatttt
      601 ttatcagaat aacactcata ataaatcatc aaaactttta cataagaaat ttataaaagt
      661 ttctaaattt aacaattcta aaatatcaat aactatattt tgtagcactt ttttatttta
      721 ttattgtttg aatatttgat ggttttcaat gcaatttcaa cataaatact atgtattagt
      781 gtatactgtt gatgaattaa agtatgataa tctatttaat aaagtatgaa gtaaatgatt
      841 ttaatgtgtc tttatggtaa aattactaat aagtaataaa attaattaaa atatatggag
      901 gtgcttataa tgtttgctgt aggatttgtt gttataattg cacttgcctt tttcatttta
      961 agtgtactat tatttaatgg aaaagccagt atgctactag caggttataa ctctatgagt
     1021 aagaaagaaa aagcacgata cgataaaaaa aaattatgta gaagttctag cattgttact
     1081 tttattgtct ctataatgct atttattatg gcatttttgg gatatagaat agagagtggt
     1141 caaatggacg aaaaaaaaat ggttcccttt tctataatat ttataatagt catactttcc
     1201 agtgtatttt ttaatatcct ctactctaac aaaaaatgta aaaaataaat agttgtaagt
     1261 tgagcagtta attataaaag attgactgct ttttcatgta tattgactac tcttattaaa
     1321 tattttaaca tttaaaaatc tatgtaaaaa taatgaatat agtacaaact tctaataatg
     1381 attttacctt tgtgg
//
LOCUS       DAMJWP010000380         1359 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.387, whole genome shotgun sequence.
ACCESSION   DAMJWP010000380 DAMJWP010000000
VERSION     DAMJWP010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.387"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(587..1180)
                     /locus_tag="PC700_003637"
     CDS             complement(587..1180)
                     /locus_tag="PC700_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2582877.1"
                     /translation="MISDSISKRRSIRKYKNQSISHETIEKIIEAGINAPSSKNRQPW
                     RFVVITEKEKESMLKAMSKGIQNEINDNGLLPGSRQHIAGANYTVEIMKQAPVTIFIL
                     NILGKSPLEKLSPEERFYEMANMQSIGAAIQNMSLTAVELGLGSLWICDVYFAYRELC
                     EWLNTDSQLVAAISLGYPDEEPSRRPRLQLSDVTEWR"
BASE COUNT          485 a          191 c          176 g          507 t
ORIGIN      
        1 tagaaaatta atctaaagaa gtataataaa ctaatttaag aaatatagaa aattaattta
       61 aagaaataca atacattaat ttttaagtaa cattaaatta atgtaagtaa aataatgttc
      121 atatgaagat ttaaaggtgc attttctgaa gctaagtata tgttatgtct ctatatccgg
      181 tgaagtttaa tagctcaaga aaatgcacta tttatgatga ttattttgtt ttatttccaa
      241 ttttggaaat aactattcta attaaatcta aaattatacc ataaataaga aaagaaagta
      301 tagatgctat gtaccaataa atcgaaattg accctgtaat tgttctacat gggatttgca
      361 tgcctgaatc cataaaaaca aaaaaactat caatggtaga aataatagac aaaataggat
      421 ttaatcctat tagaataata ctttttgagt cttgaccaat ttggtggata taaatctcaa
      481 ataatgcaat taaaaaaaac aaaatgtaaa agatttaata atttttttca tcaaaatacc
      541 tcacctaata taaattctgt ttgcactatt tgcttactat aactatttat ctccattcag
      601 tcacatcact taattgtaat ctaggtcttc ttgatggttc ttcgtcagga taacctaaag
      661 atattgctgc cactaattga ctatctgtat ttaaccattc acataattca cgatatgcaa
      721 agtatacatc acaaatccaa agactaccta gacctaattc cacagctgta agcgacatat
      781 tctgaattgc agctccaatt gattgcatgt ttgccatttc atagaatcgt tcttcagggg
      841 atagcttttc taaaggtgat ttacctaata tatttaaaat aaaaattgtt actggagctt
      901 gtttcatgat ttcaactgtg taatttgcac cagcgatatg ttgacgactt cctggaagaa
      961 gcccattatc attaatttca ttttgaatac ctttgctcat ggctttaagc atagattctt
     1021 tttccttttc agtgatgaca acaaaacgcc atggttgcct gtttttagat gaaggtgcat
     1081 ttattccagc ttcaattatt ttctctatag tttcatgtga aatgctttga tttttatatt
     1141 ttctgatact tcgtcttttt gatatagaat cacttatcat tttaagtaac ctccgtttat
     1201 tttttggaaa aatctattta ttttctattt ttaattattt tagcattaat cagaaaaaaa
     1261 gtcattgatt ttagtataca attattaacg attatattaa ggattgtatc aaaaagtatg
     1321 tgattatact aagggattat attagacatt atatttaaa
//
LOCUS       DAMJWP010000381         1352 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.388, whole genome shotgun sequence.
ACCESSION   DAMJWP010000381 DAMJWP010000000
VERSION     DAMJWP010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.388"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(128..1168)
                     /locus_tag="PC700_003638"
     CDS             complement(128..1168)
                     /locus_tag="PC700_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2582878.1"
                     /translation="MDKHSDSVMYGGMEVYNIWGTTTVDSIIGESDVMRALKNRICKI
                     GNSDSTVAITGESGTGKELIARAIHLAGNRGNKTFVAINCAAIPEPLLESELFGYTKG
                     AFSGADSKGKIGKFELANGGVVFLDEIGDMPIQLQSKLLRVLQEKKFTRIGSNELIDI
                     DVRIISATNRDLFELVKENKFREDLYYRLNVIPLEVPPLRERGDDIQILINNFIDKYS
                     RKMKKNVYHIEPDVEQMLLKYPWPGNIRELENTIELALNLLEEDGIIHKGIINRKILE
                     YFKLNNINIKLLEESDYEISMEQDILTLEEFERAYIKKVLKFYGNDTKSKKLAAKKLG
                     ISLATLYRKIDV"
BASE COUNT          422 a          249 c          133 g          548 t
ORIGIN      
        1 aaattttttt tatttttgga taataaaatg ctattacaca aaaaaacact tagaaaatag
       61 ttttccaagt gttttttatc aattttataa tttcaaattt tagaattgag aataaaaaac
      121 ctaattatta tacatctatt tttctgtaca atgtagctag tgatattcca agttttttgg
      181 cagccagttt tttagactta gtatcatttc cgtaaaactt taacactttc tttatatagg
      241 ctctttcaaa ctcttcaagt gtaagtatgt cttgttccat acttatctca tagtcacttt
      301 cctcaagtaa ctttatattt atattattta acttaaaata ttcaagtatc tttctattta
      361 ttattccttt atgtattatt ccatcttcct caagaaggtt taatgccagt tctatagtat
      421 tttccaactc tctaatattt cctggccaag gatacttaag caacatttgt tccacatctg
      481 gttctatatg gtacacgttt ttcttcattt ttctcgaata tttgtctata aaattattta
      541 ttaatatttg tatatcgtct cctctttctc taagtggagg cacttctaaa ggtattacat
      601 tcaatctata gtataagtct tctctaaatt tattttcctt aaccaactca aataaatctc
      661 tattagtagc tgatatgatt ctaacatcta tatcaattag ttcatttgaa cctattctcg
      721 taaacttctt ttcctgtaat accctcaata actttgattg tagttgaatt ggcatatctc
      781 ctatctcatc taaaaataca acccctccat tggccaattc aaacttacca attttacctt
      841 ttgaatctgc tccactaaaa gctccttttg tatatccaaa aagttcactt tctagtaaag
      901 gttctggtat agcagcacag tttatagcaa cgaatgtttt attccctctg tttcctgcga
      961 gatgaatggc tcttgctatt aattcttttc cagtgccact ttctccagtt atagctacag
     1021 tagaatcact atttccaatt ttgcaaattc tattcttaag agcacgcatt acatcagact
     1081 ctccaattat ggagtctact gttgttgttc cccaaatatt atatacttcc attccaccat
     1141 acattactga atcactgtgt ttgtccataa aatatcaact ccaaataagt attctatata
     1201 ataatcattc acattatcta tttaattata tcataacaga aaagcttagt tataatttaa
     1261 taatattatt ttaaaacaaa acttaataaa aagtatatct acttagtttt aatttgatag
     1321 atatactttc ttcattttac aaaaatcctc ta
//
LOCUS       DAMJWP010000382         1352 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.389, whole genome shotgun sequence.
ACCESSION   DAMJWP010000382 DAMJWP010000000
VERSION     DAMJWP010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.389"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            356..649
                     /locus_tag="PC700_003639"
     CDS             356..649
                     /locus_tag="PC700_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2582879.1"
                     /translation="MGFPETFKALSDPIRREILVMLRENKMSAGEISEKFNMTNATIS
                     YHLSQLKKAGLIFETKYKNFIFYEINISVFEEVMLWFSQFMEKGEKDNEKSDK"
     gene            630..1271
                     /locus_tag="PC700_003640"
     CDS             630..1271
                     /locus_tag="PC700_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HDF2582880.1"
                     /translation="MKKAINKQFILSTLICFIPFIVSIYFYNRLPNEVAIHFDNYGNP
                     DNYAPKVIAAFGVPLLMLCIHLYTWFRLENEPKKANFSNVIQNLSKWGVAILSVILQI
                     LMVLYSTGVSLESNFYTGLILGIIVILFGNYLPKCKQNYSVGIILPWTLDDEDNWNKT
                     HHLAGWIWLIGGILLIINAFINIPFYNIFVIFVIVILPFIYSYLMYKISGKNN"
BASE COUNT          507 a          133 c          199 g          513 t
ORIGIN      
        1 tacagtatat ttcttatttc attcttttct atttaaatag gacttaacta ctttttaaat
       61 tggatttagc tacttttaat ttgaaattaa aagtatatgt aatatatata agtatatcag
      121 tcattaggtt tttgctttaa caattgtggt gcaatacact tttgttaaat taaaaaaatt
      181 agataaaata agattatata aaagcgactt aagtaaattg agaatcttaa tatgaaaatt
      241 ttcaataaat atttggaaat aactatttag aaatttttct aaatagttat tgaaataaaa
      301 tggatgttat gttatattct atttagatga aattctaaat agcgaggtga tggtgttggg
      361 gtttccagaa acattcaaag ctctttcaga ccctataaga agagaaatat tggttatgtt
      421 gagggagaat aaaatgtcag caggagaaat tagtgaaaaa tttaatatga cgaatgctac
      481 aatttcttat catctatctc aactaaaaaa ggcaggactc atatttgaaa caaagtataa
      541 gaactttatt ttctacgaaa taaatatttc agttttcgaa gaggttatgc tttggttttc
      601 acaatttatg gagaagggag aaaaagataa tgaaaaaagc gataaataaa caatttattt
      661 tatctacttt aatatgtttt ataccattta ttgtatcaat atatttttat aatagattac
      721 caaatgaagt tgcaattcat tttgataatt atggaaatcc agataattat gctccaaagg
      781 ttatagcagc ttttggtgta ccattgttaa tgctatgtat ccatttgtat acatggttta
      841 gacttgagaa tgagcctaaa aaagctaatt tttcaaatgt tatacaaaat ttatcaaaat
      901 ggggagttgc tatactttca gtaattctac aaatactgat ggttttatat tcaacaggag
      961 ttagtttgga gtctaatttt tatacaggat taattttagg aataatagtt attttatttg
     1021 gaaactatct tcctaaatgt aaacaaaatt actcagtagg aataattttg ccatggactc
     1081 ttgatgatga agataattgg aataagacac accatttagc tggatggatt tggcttattg
     1141 gtggaatatt attaattatt aatgcattta ttaatattcc tttttataat atctttgtaa
     1201 tctttgtaat tgtaatacta ccatttatat attcttattt gatgtataaa atttcaggta
     1261 aaaataacta atataattaa tcactataat aatgtttgga taagaataat atatttcaga
     1321 actgagatag ataataaaga aattactaag at
//
LOCUS       DAMJWP010000383         1343 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.390, whole genome shotgun sequence.
ACCESSION   DAMJWP010000383 DAMJWP010000000
VERSION     DAMJWP010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.390"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            201..1298
                     /gene="ychF"
                     /locus_tag="PC700_003641"
     CDS             201..1298
                     /gene="ychF"
                     /locus_tag="PC700_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDF2582881.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITQAGAESANYPFCTIDPNVGVVSV
                     PDERLNKLQELYNSEKIVPTAIEFCDIAGLVRGASKGEGLGNKFLSHIREVDAIVHVV
                     RCFEDENVVHVDGSVDPLRDIETINLELIFSDIEILERRINKTQKAAKADKTLGSELD
                     LLKSIMSTLEESKCVRTMEFTEDEQTFVNSLDLLTSKPVIYASNVSEDDLADNGENNK
                     YVQQVKAFAETEDAEVVVVCAQIEAEISELDSAEEKKEFLETLGLEQSGLDKLIKSSY
                     ALLGLISFLTAGPKEVRAWTIKVGSKAPQAGGKIHSDIERGFIRAETIAFNDLVEHGT
                     MAAAKEKGLVKLEGKEYIVKDGDVILFRFNV"
BASE COUNT          521 a          149 c          243 g          430 t
ORIGIN      
        1 tctaaaagca atatttttat ttaaattaaa tgcaaatatt atttaaatca atatgcttta
       61 aagtctagtt tacaagcttg atatatatga tgtataatca atatgtatgt ctataattaa
      121 aacaattttg caatcctata gattgattgt ttaaagttat ataaagaata taaaaaagaa
      181 taagagagag gacgattaat atgaaattag gtatagttgg tttgccaaat gtaggtaaaa
      241 gcacactatt taacgcaata actcaagcag gtgcagaatc tgcaaattac cctttttgta
      301 caatagaccc aaatgtggga gttgtatctg ttccagatga aagattaaac aaactacaag
      361 aattatataa ttctgaaaaa atagttccta ctgctataga attttgtgat atagcaggtt
      421 tagttagagg tgcttctaaa ggagaaggtt taggaaataa attcttatca catataagag
      481 aagttgatgc aatagttcat gttgttagat gttttgagga tgaaaatgtt gtacatgtag
      541 atggctctgt agacccactt cgcgatatag aaactataaa cttagaatta attttctcag
      601 atatagagat tcttgaaaga agaattaata aaactcaaaa agctgctaag gctgataaaa
      661 ctttaggttc tgaacttgat ttattaaaat caataatgtc tactttagaa gaaagtaagt
      721 gtgtaagaac tatggaattt acagaagatg aacaaacatt cgtaaattca ttggatttac
      781 ttacatctaa gcctgtaata tatgcttcaa atgtatcaga agatgattta gctgataatg
      841 gagaaaataa taaatatgtt caacaagtta aagcatttgc agaaacagaa gatgccgaag
      901 ttgttgtagt atgtgcacaa atagaagcag aaatttctga acttgattct gctgaagaaa
      961 agaaagagtt tttagaaaca ttaggtcttg aacaatcagg gcttgataag cttataaaat
     1021 cttcttatgc tttacttggt ttaatttcat tcttaactgc tggtccaaaa gaagttagag
     1081 catggactat aaaagttgga agtaaagctc ctcaggcagg aggaaaaata cattctgata
     1141 tagaaagagg atttatcaga gcagaaacaa tagcctttaa tgatttggtt gagcatggta
     1201 ctatggctgc tgctaaagaa aaagggttgg ttaaattaga aggtaaagaa tacatagtta
     1261 aagatggaga tgtaatatta tttagattta atgtttaaaa tgattaatca tatacacttt
     1321 gactattata tataataact aaa
//
LOCUS       DAMJWP010000384         1336 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.391, whole genome shotgun sequence.
ACCESSION   DAMJWP010000384 DAMJWP010000000
VERSION     DAMJWP010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.391"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            27..101
                     /locus_tag="PC700_003642"
     tRNA            27..101
                     /locus_tag="PC700_003642"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Thr,seq:tgt)
     gene            116..200
                     /locus_tag="PC700_003643"
     tRNA            116..200
                     /locus_tag="PC700_003643"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Tyr,seq:gta)
     gene            209..292
                     /locus_tag="PC700_003644"
     tRNA            209..292
                     /locus_tag="PC700_003644"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:243..245,aa:Leu,seq:tag)
     gene            322..398
                     /locus_tag="PC700_003645"
     tRNA            322..398
                     /locus_tag="PC700_003645"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Arg,seq:tct)
     gene            406..481
                     /locus_tag="PC700_003646"
     tRNA            406..481
                     /locus_tag="PC700_003646"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:439..441,aa:Gln,seq:ttg)
     gene            570..658
                     /locus_tag="PC700_003647"
     tRNA            570..658
                     /locus_tag="PC700_003647"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:604..606,aa:Ser,seq:tga)
     gene            662..737
                     /locus_tag="PC700_003648"
     tRNA            662..737
                     /locus_tag="PC700_003648"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:695..697,aa:Phe,seq:gaa)
     gene            744..817
                     /locus_tag="PC700_003649"
     tRNA            744..817
                     /locus_tag="PC700_003649"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:778..780,aa:Met,seq:cat)
     gene            832..908
                     /locus_tag="PC700_003650"
     tRNA            832..908
                     /locus_tag="PC700_003650"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:866..868,aa:Ile,seq:cat)
     gene            932..1008
                     /locus_tag="PC700_003651"
     tRNA            932..1008
                     /locus_tag="PC700_003651"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:966..968,aa:Pro,seq:tgg)
     gene            1016..1092
                     /locus_tag="PC700_003652"
     tRNA            1016..1092
                     /locus_tag="PC700_003652"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1050..1052,aa:His,seq:gtg)
     gene            1101..1176
                     /locus_tag="PC700_003653"
     tRNA            1101..1176
                     /locus_tag="PC700_003653"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1134..1136,aa:Lys,seq:ttt)
     gene            1184..1257
                     /locus_tag="PC700_003654"
     tRNA            1184..1257
                     /locus_tag="PC700_003654"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1216..1218,aa:Cys,seq:gca)
     gene            1264..1335
                     /locus_tag="PC700_003655"
     tRNA            1264..1335
                     /locus_tag="PC700_003655"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1296..1298,aa:Asn,seq:gtt)
BASE COUNT          320 a          310 c          355 g          351 t
ORIGIN      
        1 ccccttccag gtcgccaaaa taaaatgctg gtgtggctca acggtagagc agctgacttg
       61 taatcagcag gttgtaggtt cgattcctat caccagctcc atgtaaaatt aatacggagg
      121 atttcccgag cggccaaagg gggcagactg taaatctgtt gtcatcgact tcggtggttc
      181 gaatccacca tcctccacca aaaaatgcgc ggatgtggcg gaattggcag acgcactaga
      241 cttaggatct agcgccattg gcgtgggggt tcgactccct tcatccgcac catttttgtt
      301 tgatatacat atataaattg tgggtgcata gctcagctgg atagagcaac gcccttctaa
      361 ggcgtgtgtc cggggttcga atccctgtgc gctcaccaca tatattgggg attagccaag
      421 tcggtaaggc acatgacttt gactcatgta tgcgtaggtt cgagtcctgc atccccagcc
      481 aaatgatgta ttactaagct atatttgaac tatttttatt ttaaatatag ttaggttcaa
      541 ttttagaata agtttaagta tccgaatgtg gagaggtgtc cgagtggttt aaggagctgg
      601 tcttgaaaac cagtgacttc gaaaggggcc gtgggttcga atcccaccct ctccgccaaa
      661 tgcccagata gctcagtcgg tagagcaggg gactgaaaat ccccgtgtcg gtggttcgat
      721 tccgcctctg ggcaccaaaa tatggcggta tagcttagtt ggctagagcg ttcggttcat
      781 acccgaaagg tcataggttc gactcctatt accgctacta aaatttaatg tgggccatta
      841 gctcagttgg ttagagcgcc cggctcataa ccggtaggtc tggggttcga gtccctgatg
      901 gcccaccaca ttaaattaat taatataaat tcgaggtgta gcgcagtttg gtagcgcaca
      961 tggtttggga ccatggggcc gggggttcga gtcccttcac ctcgaccaat ataaggtggg
     1021 tatagctcag ttggttagag cgccagattg tggctctgga ggtcgtgagt tcgactctca
     1081 ttatccaccc caattcatat ggtctattag ctcagtcggt agagcacctg acttttaatc
     1141 agggtgtccc gcgttcgagt cgcggataga tcaccaaaaa ggtggcgaca tagccaagtg
     1201 gtaaggcagt ggactgcaac tccttgatcc ccagttcgaa tctgggtgtc gcctccaaaa
     1261 tattccaagg tagctcaacg gtggagcaac cggctgttaa ccggtaggct gtgggttcga
     1321 gccccaccct tggagc
//
LOCUS       DAMJWP010000385         1288 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.392, whole genome shotgun sequence.
ACCESSION   DAMJWP010000385 DAMJWP010000000
VERSION     DAMJWP010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.392"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            18..>1288
                     /locus_tag="PC700_003656"
     CDS             18..>1288
                     /locus_tag="PC700_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HDF2582882.1"
                     /translation="MNILGLIVEYNPFHNGHIYHLSKSIEKTNATHTVAIMSGNFLQR
                     GEPALFDKYTRAKIAVENGVDLVIELPTLFACQSAEIFSHGAITILNSLNCINSICFG
                     SEEGNTEILYNIAEILANEPNEFKLLLKKYLDDGLLFPTARANALFDYIHQDKKNNFI
                     QNYSKEQLSKTLNSSNNILGIEYIKNLIHLNSSIKPFTISRVQSEYNSEEIESDICSA
                     TAIRTSLKENTDLFHLKNVIPEKTYNILNEKVDNGFLPMFDDFFFDILKTIILRDMDT
                     LKEYFEVNEGIENRIYKDIFTSEFLHELQLAIKSKRYTLTKIKRTLNNILLGIKKSDM
                     QLIKNIDTIPYIRILAFNDKGREILKEIKNKSEIKIINKFSNISFSLDDTIFKTLISY
                     DIKATNIYNSIYYKNNKDLVKGPMDFYTHPI"
BASE COUNT          547 a          170 c          158 g          413 t
ORIGIN      
        1 gataaaagga gtattttatg aacatacttg gattaatagt tgaatataac ccatttcata
       61 atggacacat atatcattta tctaaatcta tagaaaaaac aaatgcaaca catactgtag
      121 caataatgag tggaaatttt ctacaaagag gcgaaccagc tttatttgat aaatatacta
      181 gagcaaaaat tgctgtcgaa aatggtgtag acttagttat agaattacca actttatttg
      241 cttgtcaaag tgctgaaatt ttttctcatg gtgctataac aattttaaat tcactaaact
      301 gcataaattc tatatgtttt ggtagtgaag aaggaaatac agaaattctt tataatatag
      361 ctgaaatttt agcaaatgaa ccaaatgaat ttaagttact tttaaaaaaa tatttagatg
      421 atggcctgtt atttccaaca gctagagcta atgcactttt tgattatata catcaagaca
      481 aaaaaaataa ttttatacaa aattattcaa aggaacaatt atcaaaaaca ttaaattcat
      541 caaacaacat actgggtata gaatacataa aaaacctcat ccatttaaac agtagtataa
      601 aaccatttac tatctccagg gtccaatcag aatataattc cgaagaaatt gagagtgata
      661 tttgttctgc tactgctata aggacttcac ttaaagaaaa tacagacctt tttcatttaa
      721 aaaatgttat tccagaaaaa acttataaca tcctaaatga aaaagtagat aatggttttt
      781 tacctatgtt tgatgacttc ttctttgata ttttaaaaac aattatactt agagatatgg
      841 acactttaaa agagtatttt gaagtaaatg aaggcataga aaacagaatc tataaagata
      901 tctttacatc tgaatttcta catgagctac aattagcaat taaatccaag cgatacactt
      961 taaccaaaat caaacgaact ctaaacaata tattgttagg tattaaaaaa agcgacatgc
     1021 aacttataaa aaacatagat actatacctt atataagaat acttgctttt aatgataaag
     1081 gtagagaaat tctaaaagag ataaaaaata aatctgaaat aaaaataata aacaagtttt
     1141 caaatatatc attttcatta gatgatacga tttttaaaac actcataagt tatgatataa
     1201 aagctacaaa tatatataat tcaatatatt ataaaaacaa taaagattta gtaaaaggcc
     1261 ctatggattt ttatactcat ccaatata
//
LOCUS       DAMJWP010000386         1282 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.393, whole genome shotgun sequence.
ACCESSION   DAMJWP010000386 DAMJWP010000000
VERSION     DAMJWP010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.393"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(<1..547)
                     /locus_tag="PC700_003657"
     CDS             complement(<1..547)
                     /locus_tag="PC700_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582883.1"
                     /translation="MKAKNIGKYTICFIIVCFLMITMTLILSYFNKDKLSVKTLKGNV
                     KDIGRVSVVYTPNISKFSYEEVIISKNGVRNNKNPKSRIRNDYLNIDKKYSDFVNDKG
                     KELIYKDDLNIGEAYTSVKSDLVQGMNALNDCIKVSNKNLKSGVEENFDINLNKSDFK
                     ENSWKTLFNKIYKGNLYMVISE"
     gene            complement(558..>1282)
                     /locus_tag="PC700_003658"
     CDS             complement(558..>1282)
                     /locus_tag="PC700_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582884.1"
                     /translation="ILNIVKNLNDYNYIIENAVKSEQLTKIGNTIETVNVTGFSSFRN
                     IMGSTESWFIFGIIACICYAFFIWYRDFNGRSKSIYTLIMLPKNRINIYISKLLNILF
                     LVYSYTVVLTISLFIASKLLPRHMLGNVTNYGFVQETIYELKMLLPYSFEILFVEYIF
                     LLIGFVSVVFTSILINKSIYKVSTLISFLFLVLEILVFIISIEFIIPYEYSDVFTVLY
                     SSINIFVCSLISNKLLKQKIDF"
BASE COUNT          471 a          193 c          105 g          513 t
ORIGIN      
        1 tttcactaat taccatatat aaattgcctt tatatatttt attaaacaag gttttccaac
       61 tgttttcttt aaaatcagac ttatttaaat taatatcaaa attttcttct accccacttt
      121 ttaaattttt atttgaaacc tttatacaat catttaaagc attcattcct tgtactaaat
      181 cagacttaac agaggtatac gcttctccta tattcaaatc atctttatat ataagttctt
      241 ttcctttatc attaacaaaa tcactgtatt ttttgtcaat atttaaataa tcatttctaa
      301 ttcttgattt ggggttttta ttatttctta ctccattttt acttataatt acctcctcat
      361 aagaaaattt gcttatatta ggtgtatata ctacacttac tctccctata tcttttacat
      421 tgccttttaa agtttttaca gatagtttat ccttattaaa ataactcaat attaaggtca
      481 tagtaatcat taaaaaacat actattataa aacatattgt atattttcct atattttttg
      541 ctttcataat atccctccta aaagtcaatc ttttgcttca aaagtttatt agatataaga
      601 ctacatacaa atatgtttat acttgaatat agaacagtaa atacatcact gtattcataa
      661 ggtataataa attctataga aattataaac actaaaattt ccaaaactaa aaatagaaaa
      721 cttattaaag tgcttacttt ataaatagat ttatttataa gaattgatgt aaatactaca
      781 gatacaaatc ctatcaataa aaatatatac tctacaaata gtatttcaaa gctatatgga
      841 agtaacattt ttaactcata tattgtttct tgtacaaaac catagtttgt gacattacca
      901 agcatatgtc taggcaacaa cttacttgca ataaacaaag atattgtaag tactactgta
      961 tagctataca ctagaaataa tatatttaat aatttagata tataaatatt gattctattt
     1021 ttaggtagca ttataagtgt atatatagat ttactcctac cattaaagtc tctataccaa
     1081 ataaaaaaag catagcaaat acatgcaatt ataccaaata taaaccacga ttctgtagaa
     1141 cccattatat ttctaaagct tgagaaacct gtaacattta ccgtttctat cgtatttccg
     1201 atttttgtta actgttcact tttaactgca ttttctatta tgtaattata gtcatttaaa
     1261 ttttttacta tgtttaaaat ta
//
LOCUS       DAMJWP010000387         1259 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.394, whole genome shotgun sequence.
ACCESSION   DAMJWP010000387 DAMJWP010000000
VERSION     DAMJWP010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.394"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..1146
                     /locus_tag="PC700_003659"
     CDS             <1..1146
                     /locus_tag="PC700_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HDF2582885.1"
                     /translation="TIEVRNAGPSTAQNVTLTDNIPASILSPEYSIDNGVTFQPWNGS
                     LSIGTLDAGEIRNIIIRGIVSQTAIGTIINTATVSSTTPDPNLNNNTSTSEAEVSSSA
                     DISVVKRSNQTVVVPGDVLDYTIEVINAGPSTAQNVTLTDNIPASILSPEYSIDNGVT
                     FQPWNGSLSIGTLGAGEIRNIIIRGIVSQTAIGTIINTATVSSTTPDPNLNNNTSTSE
                     IDISSSADISVIKLANKTEACVEEQIDFVIVVSNAGPENAQNVTLIDNVSDKLKKAVF
                     SLDRGVSFQPWTGSLNIGTLPAGTLRVILLRGIIKSTCLDRLTNIAEVTSTTPDSNLN
                     NNISRVQVEIKQCCCNDCCCNNCSNCCCNDCGCKDCCCNCCKNDFWC"
BASE COUNT          494 a          178 c          218 g          369 t
ORIGIN      
        1 acaattgagg tgagaaatgc aggaccgtct actgctcaga acgtaacttt aacagataat
       61 ataccagctt ctattttaag tccagaatat tcaatagata atggagtgac ttttcaacca
      121 tggaatggaa gtcttagtat aggcacttta gatgctggag agataagaaa tattataata
      181 aggggaatag taagtcaaac agcaataggt acaattataa atacagcaac agtaagttca
      241 actacacctg acccaaatct aaataacaat acttcaacat cagaagcaga ggtaagttca
      301 tcagcagata tttctgtagt aaaacgaagc aatcagactg ttgtcgtacc aggcgatgta
      361 ctagactata caattgaggt gataaatgca ggaccgtcta ctgctcagaa cgtaacttta
      421 acagataata taccagcttc tattttaagt ccagaatatt caatagataa tggagtgact
      481 tttcaaccat ggaatggaag tcttagtata ggcactttag gtgctggaga gataagaaat
      541 atcataataa ggggaatagt aagtcaaaca gcaataggta caattataaa tacagcaaca
      601 gtaagttcaa ctacacctga cccaaatcta aataacaata cttcaacatc agaaatagat
      661 ataagttcat cagcagatat ttctgtaata aaattagcaa ataaaacaga agcatgtgta
      721 gaagagcaaa tagattttgt aatagtagta tcaaatgcag gaccagaaaa tgctcaaaat
      781 gttactttaa tagacaatgt ttcagataag cttaagaaag cagtattttc acttgataga
      841 ggtgtaagtt tccaaccatg gactggaagt cttaatatag gtacactacc agctggaact
      901 ttaagagtta ttctattaag aggaattata aaatcaacat gtcttgatag attaacaaat
      961 attgcagaag ttacttcaac aacaccagat tcaaacctta acaataatat atcaagagtg
     1021 caagtagaaa taaaacaatg ttgttgtaat gattgctgtt gtaacaactg tagtaattgt
     1081 tgttgcaatg attgtggttg taaagattgt tgctgtaatt gttgtaaaaa tgacttttgg
     1141 tgttgatttt ataaaataat tttgtatacc ttaaattaaa ctgaaataaa aaataaattc
     1201 acatatgagt aaaatatttt ataaatatta ataaattttc tatctttgat atggaccac
//
LOCUS       DAMJWP010000388         1257 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.395, whole genome shotgun sequence.
ACCESSION   DAMJWP010000388 DAMJWP010000000
VERSION     DAMJWP010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.395"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            106..>1257
                     /locus_tag="PC700_003660"
     CDS             106..>1257
                     /locus_tag="PC700_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582886.1"
                     /translation="MKLKSKISILIIFLTLAIYFSIGLLNNEDNLKIKTIKGDSNELD
                     HININFGYALNGYINSYYTINGSNKTKKTELIKLQKDSKNTNNLIDVSSGSDLIAHVG
                     FEMNEESKDINFVIDEKKEDLEKGVFPSEGSDKEIEYEKLDMNNFTRTRYKVDKSTDD
                     YYANILASKRYKKYLYVSVLYATSDSSENNKEYSSTIDIIKINPENKKIKTIKSINLS
                     NYFSEKYQDIENIGVSYASKYEDKMYILVNVLNTNIGDNSNSKFHLLEYNLEENKESI
                     MELKTDSKMALAKAKLEANKLYMVMSNDYDNSSITELNLLTYNILDKSLDEDKYVLDN
                     REKRGIRSIEIDSNKIVAYTSRLNSILSWDNKFMLYIINKNSKEIVYEGE"
BASE COUNT          567 a           98 c          191 g          401 t
ORIGIN      
        1 caatttagta gcagggttat taagtttttt aattataatg tttattctaa atggaatatc
       61 ttatttgcta attaaaaaga aagtatctct ttaaggaggg gtggtatgaa attaaaatct
      121 aaaatatcta tacttattat atttttgact ttagcaatat attttagtat aggtttatta
      181 aataatgaag ataatttaaa gataaaaact ataaaaggtg actctaatga attagaccat
      241 ataaacataa attttggata tgctttgaat ggatatataa attcatatta tacaataaat
      301 ggaagtaaca aaactaaaaa gactgaatta ataaaactac aaaaggatag taagaacaca
      361 aataatttaa ttgatgtatc tagtggttca gatttaattg ctcatgttgg atttgaaatg
      421 aatgaggagt caaaagatat aaactttgtg atagatgaga aaaaagaaga tttagaaaaa
      481 ggtgtatttc catcagaagg tagtgataaa gaaattgaat atgaaaaatt agatatgaat
      541 aattttacaa ggacaagata taaagtagat aaaagtacag atgattatta tgcaaatata
      601 ctagcatcaa aaagatataa aaaatactta tatgtatctg tactttatgc tacaagtgat
      661 tcatcagaaa ataacaaaga atattcaagt actatagata taataaaaat taatccagaa
      721 aataaaaaaa tcaagactat aaaaagtata aatttaagta attatttttc cgagaaatac
      781 caagacatag agaacatagg tgtaagttat gctagcaaat atgaggataa gatgtatatt
      841 ttagtaaatg tactaaatac aaacataggt gataatagta attctaaatt tcatttactt
      901 gagtataatc ttgaagaaaa taaggaaagc ataatggaat taaaaactga tagcaaaatg
      961 gctttagcaa aagcaaagtt agaagctaat aaattatata tggtaatgtc gaatgattat
     1021 gataatagtt caattacaga gttaaattta cttacctata atatattaga taaaagctta
     1081 gatgaagata aatatgtttt agataataga gaaaaaagag gaattagaag tattgaaatt
     1141 gattcaaata aaatcgttgc atatacatca aggctaaact ctatattatc ttgggataat
     1201 aaatttatgc tatatataat aaataaaaat tctaaggaaa ttgtatatga aggagaa
//
LOCUS       DAMJWP010000389         1245 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.396, whole genome shotgun sequence.
ACCESSION   DAMJWP010000389 DAMJWP010000000
VERSION     DAMJWP010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.396"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            323..700
                     /locus_tag="PC700_003661"
     CDS             323..700
                     /locus_tag="PC700_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2582887.1"
                     /translation="MKEEILIEKLLRAELIVMEYLCKKDTLMPKKEIVKEIKQIYGWH
                     KSTIKILLKRLVAKRYLARYIINFQYHYKIIIDNKEYYTFKKKVLKSSKSIKIMRSLT
                     TTHKNVSKEKLDLLDEYYRNLKE"
     gene            805..1180
                     /locus_tag="PC700_003662"
                     /pseudo
     CDS             805..1180
                     /locus_tag="PC700_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
BASE COUNT          563 a          106 c          172 g          404 t
ORIGIN      
        1 caaaaataaa atatgctttt agtaacttgt taacataact tataatttat tttataagca
       61 ttagcctata tagttttgtg ctactaaatt tctttgattc taacgattta gtgctattct
      121 acaactaaaa taatacaata gagctattta ttgtttggta gcattgtttg caaattaaac
      181 aaatatatat atatgaatat tattttacgt ttaaaaaaac agactaaata tgtagaatat
      241 ttggtaaaac ttacctctag tttttgtctt aaaaagctcc tattattaat ttaagttaga
      301 agaattaagg aagtgacata aattgaaaga agaaatatta atagaaaaat tattaagagc
      361 agaattaata gtaatggagt atttatgtaa aaaagacact ctaatgccta aaaaagaaat
      421 tgtaaaagaa ataaaacaga tttatggatg gcataaaagt acaataaaaa tactactaaa
      481 aaggctagtt gctaaaaggt atttggctag atatattata aattttcaat atcattataa
      541 aataataata gataataaag aatattatac ttttaagaaa aaggtactaa aatctagtaa
      601 aagcataaaa ataatgcgtt cacttaccac tacacataaa aatgtaagta aagagaagtt
      661 agacctatta gatgagtatt atagaaattt aaaagaatga tatagtaaaa atcgaatgta
      721 attatttacc ttaattcttt tgtttgtaaa actttattga tatagaattt aacatataca
      781 taaattggta aagggatgta tactatgaaa attagtaaat taccagaagc ggaattaaaa
      841 gttatgagat atatttggga atcagaaaag gtattaatat caagagaaat caccaagtct
      901 atggaacaaa aatatgaatg gaaagataca actatattta cagtattaaa aagattacta
      961 aagaagagag tttttagaaa cggagaaaat agataagcgt acacactata atgtattagt
     1021 aaaagaaaaa taatatcaga gattttaaac aaaagaattt ttaaagaata cacataaaaa
     1081 ttcaatatta agtttaatgt cagcactatg tagtaaggat gatgaagtag atggtgatat
     1141 tttaaattta ttagaagaat attttaagaa tttaaaataa gaaaatgaca gctagataca
     1201 taataaataa aatgaaggta tgaaattata actttttcta cattc
//
LOCUS       DAMJWP010000390         1241 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.397, whole genome shotgun sequence.
ACCESSION   DAMJWP010000390 DAMJWP010000000
VERSION     DAMJWP010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.397"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(92..1012)
                     /locus_tag="PC700_003663"
                     /pseudo
     CDS             complement(92..1012)
                     /locus_tag="PC700_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428810.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
BASE COUNT          417 a          169 c          159 g          496 t
ORIGIN      
        1 aacaaataat aaaaaaatta atttgattgt atataaatta gtttgttatt aaattagtat
       61 taaaaagttc aaaaatttac ttagaaagaa tctataattc gatttctatg tttgttgaga
      121 atgcaattac agcttttcca attattttta cttcaactgg cttattatct gaaataatta
      181 cttttactaa catcttgcct ttgcgtttta ttgcttctcc ttgaactgct tcaaatataa
      241 actctgtgtt gggtataggt attactttat aatgtacaag gtacgcacct aaaggtccat
      301 tagcatttcc tgtaacaggg tcttctgaaa taccaattgc aggagcaaac attcttccat
      361 gaacttgtag agtttcttta ttatgtaatg taaaaatata atatccatta cattttattt
      421 cttgacttat tttcgataat tcatataaat ttggagataa actatttaat gttgataatt
      481 cctttattcc aatcataact tttgaatgtc ctgtagatga tatggcaata ggacaatctg
      541 tacgaatatc ttcatttaaa ataccaaatg cattaagtaa tcttttttga taggtttgtt
      601 gtaatggtgg agatacttca atttttcctt gtgtcataat tatttcataa tcattatctt
      661 tttttataat atcaactggt aatattccag caccagtttt ttgaaatact gttttgttat
      721 tgaattggtt ttctactgct ctaacataat gggcagcaat agtagcatga ccacatatag
      781 gaacttcatt agttggtgta aaaaatctga tatgtacatc ataattttct tgatgatttt
      841 tagccaattt attgaaaata aaagcagttt atgaattatt taattctctt gttatttttt
      901 gcatttgtga ttcagataag ccatcagcat ttgtaattac accagctgga ttacctgtga
      961 atctttcttt tgtaaatgag tcaacttgat aaagacgaaa ttttttattc atattatata
     1021 atcctttcta aacattagat tagtttatct acatagaaaa cttgagataa attattattt
     1081 attattatct gtttaaataa aattaataaa ataacatatg tctatattaa aatttaaaaa
     1141 tatacatcta aaaaagtagt attatgtaag gctctatgct acactgaacc tcccatgact
     1201 aaagtcacag ggttcctgct tcatagaatt ttgcatacta a
//
LOCUS       DAMJWP010000391         1233 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.398, whole genome shotgun sequence.
ACCESSION   DAMJWP010000391 DAMJWP010000000
VERSION     DAMJWP010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.398"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            78..194
                     /gene="rrf"
                     /locus_tag="PC700_003664"
     rRNA            78..194
                     /gene="rrf"
                     /locus_tag="PC700_003664"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            202..276
                     /locus_tag="PC700_003665"
     tRNA            202..276
                     /locus_tag="PC700_003665"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:234..236,aa:Asn,seq:gtt)
     gene            282..367
                     /locus_tag="PC700_003666"
     tRNA            282..367
                     /locus_tag="PC700_003666"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:316..318,aa:Leu,seq:taa)
     gene            383..458
                     /locus_tag="PC700_003667"
     tRNA            383..458
                     /locus_tag="PC700_003667"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:416..418,aa:Met,seq:cat)
     gene            466..540
                     /locus_tag="PC700_003668"
     tRNA            466..540
                     /locus_tag="PC700_003668"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:499..501,aa:Glu,seq:ttc)
     gene            555..628
                     /locus_tag="PC700_003669"
     tRNA            555..628
                     /locus_tag="PC700_003669"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:587..589,aa:Gly,seq:tcc)
     gene            634..709
                     /locus_tag="PC700_003670"
     tRNA            634..709
                     /locus_tag="PC700_003670"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:667..669,aa:Val,seq:tac)
     gene            715..791
                     /locus_tag="PC700_003671"
     tRNA            715..791
                     /locus_tag="PC700_003671"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:749..751,aa:Asp,seq:gtc)
     gene            802..876
                     /locus_tag="PC700_003672"
     tRNA            802..876
                     /locus_tag="PC700_003672"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:834..836,aa:Gly,seq:gcc)
     gene            886..959
                     /locus_tag="PC700_003673"
     tRNA            886..959
                     /locus_tag="PC700_003673"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:920..922,aa:Arg,seq:tct)
BASE COUNT          351 a          261 c          290 g          331 t
ORIGIN      
        1 taactctaaa gaaactagta ttgctaggtt ctataaactt attaaaaact taatagaaat
       61 attaagcata aagattatgt ggttacaata gcagagagga tacacctgtt cccattccga
      121 acacagaagt taagctctct agcgctgatg gtacttggtg ggaaactgcc tgggagagta
      181 ggacgtagcc acgttaaatg ttccaaggta gctcaacggt ggagcaaccg gctgttaacc
      241 ggtaggctgt gggttcgagc cccacccttg gagccaaaaa agccgaagtg gcggaactgg
      301 cagacgcaca ggacttaaaa tcctgcggga cttacctctc gtaccggttc gattccggtc
      361 ttcggcactt ataaaacaat atcgcggggt ggagcagttg gcagctcgtc gggctcataa
      421 cccgaaggtc gtaggttcaa gtcctgcctc cgcaaccatt aaaacggctc gttggtcaag
      481 aggttaagac accgcccttt cacggcggta acaggggttc aattccccta cgagtcacca
      541 attcaattaa atatgcgggt gtagctcaat ggtagagttc tggccttcca agccagctgt
      601 gagggttcga tccccttcac ccgctccaaa aatgggacca tagctcagct gggagagcac
      661 ctgccttaca agcagggggt cacaggttcg agccctgttg gtcccaccat tcgcggcctg
      721 gtagttcagt tggttagaat gccagcctgt cacgctggag gtcgagggtt cgagcccctt
      781 ccaggtcgcc aaattaaata agcgggaata gttcagtggt agagcgcaac cttgccaagg
      841 ttgaagtcgc gagttcgaat ctcgtttccc gctccataat aacttgggtg catagctcag
      901 ctggatagag caacgccctt ctaaggcgtg tgtccggggt tcgaatccct gtgcgctcat
      961 taagaaaagt ttactataaa ttatagtaaa cttttatatt gtttaaaaag acacaggaaa
     1021 catgtatatt acctgtgtct ttttatttat aaactttaaa ctatgtaaaa attacagtat
     1081 attctaatag agttacagta tattcattaa tattgtaaac atacggaggg taatgtataa
     1141 tagtctacaa gatgtttatg aaggcatcag ttaaatataa ctaattagat aagagttata
     1201 tacattatat tgtatacttt atggtattta tat
//
LOCUS       DAMJWP010000392         1225 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.399, whole genome shotgun sequence.
ACCESSION   DAMJWP010000392 DAMJWP010000000
VERSION     DAMJWP010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.399"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(117..383)
                     /locus_tag="PC700_003674"
     CDS             complement(117..383)
                     /locus_tag="PC700_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HDF2582888.1"
                     /translation="MIESRCGILCYECGYKEKVNCKGCTDIEKPFWGEQCPVKSCCED
                     KNLIHCGLCDTFPCEMLNQFAYDKEQGDDGKRISQCKKWAVLAL"
     gene            complement(426..1181)
                     /locus_tag="PC700_003675"
     CDS             complement(426..1181)
                     /locus_tag="PC700_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2582889.1"
                     /translation="MPHKLLRQYIAHYTISIPDTSIKEKLTLIPDASGCMIFKFDKKG
                     IESAFWGATTKTTIVKNDIENVLFRVFVEFRPGGVYYLTGLSQRESTDLKISLEDFNT
                     LFSLEVNSIFERTSTIKELVEQLDMLFLSYLLKSNIVDMTIPILENARKQNSIMSVKN
                     ISQISCYSERHLNRIFNNSLGMSVKSYLRLLRINLVLQEVQNNKRPFATLAQDIGYYD
                     QSHFINDFKSICGVNPTTYIKNLSDFYNEKYKF"
BASE COUNT          421 a          183 c          146 g          475 t
ORIGIN      
        1 ttctattaat ttaattaaat aacctttaaa tttaattata aaaactgttg cagaaatttc
       61 tacaacagtt taaaaaattt accttacaaa aacttatttt ataacaagag agtatattat
      121 aaggctagta cagcccattt tttgcactga gaaattcttt tgccatcatc accttgttct
      181 ttgtcatatg caaattgatt aagcatttca catggaaatg tatcacatag accacaatgt
      241 ataaggtttt tatcttcaca acaagatttt acaggacatt gttcacccca aaatggtttc
      301 tcaatatctg tacaaccttt acaattgact ttttccttgt atccacattc ataacataaa
      361 attccacatc ttgattctat cattttaagt cactccttaa atataaattt tattatgatt
      421 gtatatcaaa atttatactt ttcattgtaa aaatcggaca aattttttat ataggttgtt
      481 ggattaacac cacaaataga tttaaaatca tttataaaat gagattggtc gtaataacca
      541 atatcttgtg ctaaagttgc aaatggtctt ttattgtttt gtacttcttg taatacaagg
      601 tttatacgaa gtaatctaag gtatgattta acactcatcc caagagaatt attaaaaatt
      661 ctatttaaat gtcgttcact ataacaacta atttgtgaaa tgtttttaac agacataata
      721 gagttctgtt ttctagcatt ttcaagaatt ggtatagtca tatctactat atttgatttc
      781 aataagtatg ataagaataa catatctaat tgttctacta attcctttat agtacttgtt
      841 ctttcaaaaa tggaattaac ctctaagcta aataatgtgt taaaatcttc aagtgaaatt
      901 tttaaatctg tagattctct ttgagaaaga cctgtcaaat aatatactcc acctgggcga
      961 aactcaacga atactctaaa aagtacattt tctatatcat tttttacaat agtagttttt
     1021 gttgtggctc cccagaatgc gctttctata ccttttttgt caaatttaaa tatcatacat
     1081 ccactagcat caggtattaa tgtaagcttt tctttaatac ttgtatctgg aatagatatt
     1141 gtatagtgtg ctatatactg tcttaacaat ttatgtggca aaatatatgt atactgtttg
     1201 caggtattta aaatgttatt gatta
//
LOCUS       DAMJWP010000393         1213 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.400, whole genome shotgun sequence.
ACCESSION   DAMJWP010000393 DAMJWP010000000
VERSION     DAMJWP010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.400"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(<1..497)
                     /locus_tag="PC700_003676"
     CDS             complement(<1..497)
                     /locus_tag="PC700_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2582890.1"
                     /translation="MLTNLSIGFWNIINLVSGTIDWIIFFLVIHLVGKRKISNIRYIF
                     TTIIIFIFMAYINVVKVFPNIKILLCMVIGISFYITSYEDKIYKSIIVSLLFWLGLMI
                     AEGSAVGLVVFINKLNNINITQHENLFRIQAIIMSKVFLFIGFILFKYFKLSLDFKPK
                     DMILIG"
     gene            complement(491..1201)
                     /locus_tag="PC700_003677"
     CDS             complement(491..1201)
                     /locus_tag="PC700_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2582891.1"
                     /translation="MISIGICDDELHYRIKIKDILSEILSSYPINYNIYEFSSGEELL
                     NNYPKDLDILIMDIQMKTINGMDTARKIREFDHKLEIIFVTSFVEFMQEGYEVKAYRY
                     ILKPINKEKISKSVLPCINEMMKKRNNYLTINVKNYVDRIKIDSITYIETDRPNILIY
                     THDDMYITKMSISKIEKILNEYGFFRCHNSYIVNLKLVESMSGSTVIVDGKSIPISKY
                     RVKGLKLAITNILGDIVC"
BASE COUNT          432 a          182 c           92 g          507 t
ORIGIN      
        1 cctattagta tcatatcttt aggtttaaaa tctaaagata atttaaaata cttaaacaat
       61 ataaaaccta taaataaaaa tacctttgac ataataatag cttgtattct aaataagttt
      121 tcatgttgtg ttatgttaat attatttaat ttatttataa atactactag acctactgca
      181 gaaccttcag caatcattaa tcctagccaa aacaataaac taactattat acttttatat
      241 attttatctt catatgatgt tatataaaat gatattccta ttaccataca tagaagaatc
      301 ttaatatttg gaaacacttt aactacattt atgtatgcca taaatataaa tataattatt
      361 gttgtaaata tatatcttat attagatatt ttcctctttc caactaagtg gattactaaa
      421 aaaaatataa tccaatctat agtacctgaa actaaattaa taatattcca aaatcctata
      481 cttagatttg ttagcatact atatccccta atatattagt tatagcaagt ttcaagcctt
      541 taactctata tttacttatt ggaatacttt tgccatctac tataacagta cttccactca
      601 ttgattctac taattttaaa ttaactatat agctattatg acatctaaaa aatccatatt
      661 catttaatat cttttctatt ttagatatac tcattttagt aatatacata tcatcatgtg
      721 tataaatcaa tatattcggt ctatctgttt ctatataagt aatagaatct attttaattc
      781 ggtctacata attttttaca tttatagtta agtaattatt tctctttttc atcatctcat
      841 ttatacaagg taatacactt ttagaaattt tttctttatt tattggcttt agtatatatc
      901 tgtatgcttt tacctcataa ccttcttgca taaactctac aaatgaagta acaaatatta
      961 tttctaactt atgatcaaac tctcttatct tcctagcagt atccatacca tttattgttt
     1021 tcatttgtat gtccataatt aatatgtcta aatctttagg atagttattt agtaactctt
     1081 ctcctgatga aaattcatat atgttataat ttataggata tgaacttaat atttcactta
     1141 atatatcttt tatttttatt cgataatgta gttcatcatc acatattcct atactaatca
     1201 caattcttaa aca
//
LOCUS       DAMJWP010000394         1202 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.401, whole genome shotgun sequence.
ACCESSION   DAMJWP010000394 DAMJWP010000000
VERSION     DAMJWP010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.401"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            71..1111
                     /locus_tag="PC700_003678"
     CDS             71..1111
                     /locus_tag="PC700_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDF2582892.1"
                     /translation="MNLNLTNTINLMKKYLDIPSPAGYTENAVLEIKKDFENLGLDTI
                     LTKKGALIATLPGEDDSNQITISAHMDTLGAIVKEISSDGTLKYHKVGGGCWAAIEGE
                     NCTVITRKGKKIRGSVVFKYASTHIYGQTKAATERNEETMIIRLDEKVFTKQDVLDLG
                     ISVGDFVCLDHRFEATESGFIKSRYIDNKSAVAMVLEICRYFKENNLTPKHTTNFYIS
                     NYEEIGHGVSKCLPEKTKEFVAIDIAPTGPSQTSSEHGVTIVAKDNLSLYDFNLRNKL
                     VDVAEDNNLDYAVDVFTFYGSDASEAIRWGEDVQIACVGPGTNNSHHYERTHIEAIEN
                     TLKLLINYMLKE"
BASE COUNT          467 a          143 c          197 g          395 t
ORIGIN      
        1 attttagttt tcagaacatt tgtatttatg ttataatttg tataaatata tttatttagg
       61 aggaaacaat atgaatttaa atttaactaa tactataaat ttaatgaaaa aatatttgga
      121 cattccaagc ccagctggat atactgaaaa tgcagtttta gaaatcaaaa aagattttga
      181 aaatttaggt cttgacacta ttcttacaaa aaaaggtgca cttatagcta ctcttcctgg
      241 tgaagatgat tcaaatcaaa taacaatatc tgcacatatg gatacacttg gagctatagt
      301 aaaagaaata tcatctgatg gtactcttaa ataccataaa gttggtggtg gatgttgggc
      361 agctatagag ggagaaaact gcactgttat aactagaaaa ggtaaaaaaa tcagaggaag
      421 tgttgtattt aagtatgctt ctactcatat atatggtcaa acaaaagctg ccactgaaag
      481 aaatgaagaa acaatgatta ttagacttga tgaaaaagta tttacaaagc aagatgtatt
      541 agatttagga ataagtgttg gggattttgt atgcctagac catagatttg aggctacaga
      601 atctggtttt ataaaatcta gatatataga taataaatca gctgtagcaa tggttcttga
      661 aatatgtaga tattttaaag aaaacaattt aacgcctaag catactacta acttttatat
      721 aagtaattat gaagaaattg gacatggtgt ttctaaatgt ttacctgaaa aaacaaaaga
      781 atttgtagca atagatatag cacctactgg accaagtcaa acatctagtg aacatggtgt
      841 aactatagtt gcaaaagata atttatcatt atatgatttt aatttaagaa acaagcttgt
      901 cgatgttgca gaagacaata atttagacta cgctgtcgat gtatttactt tctatggttc
      961 agatgcttca gaagctattc gttggggaga agatgttcaa atagcatgtg ttggtcctgg
     1021 aacaaacaat agtcaccatt atgaaagaac acatatagaa gctattgaaa atacactaaa
     1081 attacttata aattatatgc ttaaagaata attaattata tattttacca aagtcaattc
     1141 ttaacaaata attaacttta atttttattt ttatacaaaa attggttttt ctatagactt
     1201 aa
//
LOCUS       DAMJWP010000395         1197 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.402, whole genome shotgun sequence.
ACCESSION   DAMJWP010000395 DAMJWP010000000
VERSION     DAMJWP010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.402"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            1..468
                     /locus_tag="PC700_003679"
     CDS             1..468
                     /locus_tag="PC700_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HDF2582893.1"
                     /translation="MITKFFSNILSVFLYFLLSLLGVAGIIFLCMIPMFLYDFSSDKV
                     IALIARILYFSDYFIVILFLIFIINSTKYSPFILENVKRFKVMGCCLLINTIIECVIG
                     YNSNTSLIMIFGDNSGGISPLMVISFIFALMCFVIAETFDKAIKIKEDNDLTI"
     gene            479..694
                     /locus_tag="PC700_003680"
     CDS             479..694
                     /locus_tag="PC700_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2582894.1"
                     /translation="MAIVVNLDVMMAKRKISLKDLAEKIDITNANLSILKTGKAKAIR
                     FTTLNEICKALDCQPGDILEYVEDEND"
BASE COUNT          428 a          142 c          154 g          473 t
ORIGIN      
        1 atgattacaa aattttttag taatatatta tcagtatttc tatattttct attatcactt
       61 ttaggagttg ctggtataat attcttatgt atgataccta tgtttttata tgattttagt
      121 tctgacaaag taatcgcttt aattgcaaga atactatatt tttctgacta ctttatagtc
      181 atcttattct tgatatttat aattaatagt actaagtatt ccccattcat acttgaaaat
      241 gtaaaaaggt ttaaagtaat gggatgttgt ttattgataa acactataat tgaatgtgta
      301 attggttata actcaaatac tagtcttata atgatatttg gagataatag tggcggtatc
      361 tcaccactta tggttatttc ttttatattt gcactaatgt gctttgtaat tgcagaaaca
      421 tttgataaag ctataaaaat aaaagaagat aatgatttaa cgatatagag gtgatgttat
      481 ggcaatagta gttaacctag atgtaatgat ggcaaaaagg aaaatatcac ttaaagattt
      541 ggcagaaaaa atagatatta caaatgcaaa tttatcaatt ttaaaaactg gaaaagcaaa
      601 agctattaga tttaccactc taaacgaaat ttgcaaagct ttagactgtc agccaggtga
      661 tatacttgaa tatgtagaag atgaaaatga ttaaaaaaga gtgtttagaa acactctttt
      721 ttattatact atatgatttt tattctttta ctatattatt gacattaata ttaactttgt
      781 ttaaagtcat aacaaaatac gttctaagaa agaaactgtt cctttttcta tttgttatcc
      841 attacctata agaactgtat cacactaaat catactttta aattgataat tttgccacag
      901 attcctatat acattctttt ctacctaata tttatttaaa ctgttagcaa aacatggaac
      961 tttttcattt acttatacct ccatttatat tattgcatag tttatagcta cgtgttataa
     1021 attacattat tgatgattta tctaacattt gtaatctttt agatactctt tgaagttaca
     1081 acactgttat actctaatta agtaaactaa atctgataca agttggtgat tatatgaaaa
     1141 gaaaactttc aacctatttt aaaggatata ttttaggttt atgtatagca tttttat
//
LOCUS       DAMJWP010000396         1157 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.403, whole genome shotgun sequence.
ACCESSION   DAMJWP010000396 DAMJWP010000000
VERSION     DAMJWP010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.403"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            3..1142
                     /locus_tag="PC700_003681"
     CDS             3..1142
                     /locus_tag="PC700_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDF2582895.1"
                     /translation="MVIKLTIEEYKNKYSTGDKIILSLKSFDNKELYYHETDNILNSK
                     DEIDRLTSSKIFKIIEDKNDLALESYYSSVIEGAFSTRKIAKSIIRGKMKPSNKSEYM
                     IYNNHRALEYGLDNLDKLYSHKFIYNLHHILGENCLDSEEYEYRTEKVYVCDSKGEII
                     HTGLDPLKIYNFMDELIIFMENSKVNDLVKSTIIHFYFVYVHPFSDGNGRTSRALSYL
                     YLINKGYDTFKEFSISYIISKNRTKYYKAILDVENKGYNLTVFIEFMLKSIIQSINEM
                     RDMHDRKSLESILKEELFENDITLSATEEHILKYICSKDNYSMTLENYIKKNKGRYLK
                     AGIKEIELVDQLMEVFNNLEEIEILSREKDVYKVNEKYLNMLEIS"
BASE COUNT          502 a           99 c          184 g          372 t
ORIGIN      
        1 aggtggtgat taaactgaca atagaagaat acaaaaataa atactctact ggagataaaa
       61 ttatactttc cttaaagagt tttgataata aagaactata ctatcatgaa actgataaca
      121 tattaaattc aaaagatgaa atagatagat taacatcaag caagatattt aaaataatag
      181 aagataaaaa tgatttagcc ttagagtctt attattctag tgtaatagaa ggtgcctttt
      241 caactagaaa gattgcaaaa tctataatta gaggaaagat gaaaccaagt aataaaagtg
      301 agtatatgat ttacaataat catagagcct tagaatatgg attagataat ttagataaat
      361 tatatagtca caaatttata tataatttac atcatatatt gggtgaaaac tgtttagatt
      421 cagaagaata tgaatataga actgaaaaag tatatgtatg tgattcaaaa ggtgagatta
      481 tacatacagg acttgatcct ttgaaaatat ataattttat ggatgaatta ataattttta
      541 tggaaaatag taaagtaaat gatttagtaa agagtacaat aattcatttc tactttgtat
      601 atgttcatcc ttttagtgat ggtaatggaa gaacatcaag agccttatca tatctatatt
      661 taattaataa aggatatgat acttttaaag agttttcaat atcatatata atatctaaaa
      721 acagaactaa gtattataag gctatactag atgtagagaa taaaggatat aatttaactg
      781 tatttataga gttcatgcta aagtctataa tacaatctat caatgaaatg cgtgatatgc
      841 atgataggaa gtctttagag agtatattaa aagaggaatt atttgaaaat gatataactc
      901 tatcagcaac agaagaacat atattaaaat atatctgtag taaagataat tattctatga
      961 cattagagaa ctatatcaaa aagaataaag gtagatactt aaaggctgga ataaaagaaa
     1021 tcgaattagt tgaccagttg atggaagtgt ttaataatct tgaagaaata gagattttat
     1081 ctagagaaaa ggatgtatat aaagttaatg agaagtactt gaatatgctt gaaataagtt
     1141 aaattgaagc atataaa
//
LOCUS       DAMJWP010000397         1152 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.404, whole genome shotgun sequence.
ACCESSION   DAMJWP010000397 DAMJWP010000000
VERSION     DAMJWP010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.404"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            333..845
                     /locus_tag="PC700_003682"
     CDS             333..845
                     /locus_tag="PC700_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582896.1"
                     /translation="MKVKKFKSGIVMALTVMAIGASLTNVSAMELNKSKEFETKYNEM
                     KRDFQEDYGKEDKFNITLDDEFTKEEVNGAKGIIKTTNKMNNEVVVLNYFDEMKSGIE
                     FELKAKAEKNAILRDEFQQGYGKEDKFKVVLDDEFTKEEVNGAKGIIKTTDKETGEVI
                     VYNYFDELEK"
BASE COUNT          522 a           81 c          204 g          345 t
ORIGIN      
        1 aaagtaaata aattattgtt aaagaatgta aagactttta aattttttat cacaggattt
       61 taagtaaata agaaggattg gagtaattaa aaatttttat tttgcaaaaa taagtattta
      121 tttatatgga taaatttgat tttaaatcta tcaatataga taaaatctta ttatttaact
      181 tcaactattc cttaaaaaag caacttttaa ggaatagttg aaacaaaaag tttggttaca
      241 taatagtaat tttagttaca agaatgaaac tgtaaaactt aggattttct ttatataatt
      301 tcattgagaa catttaattt aggaggtaag gtatgaaagt aaaaaaattt aaaagtggta
      361 tagtaatggc attaacagta atggcaattg gagcatcatt gacaaatgta tcagctatgg
      421 aattaaacaa gagtaaagag tttgaaacta aatacaatga aatgaagagg gattttcaag
      481 aggattatgg aaaagaagac aaatttaata taacattgga tgatgaattt acaaaagaag
      541 aagtaaatgg agctaaaggg attataaaga caactaataa aatgaataat gaggttgtag
      601 tattaaatta ttttgatgaa atgaaatctg ggattgaatt tgagttgaag gcaaaagcag
      661 aaaaaaatgc aattttgaga gatgaatttc aacaaggtta tggaaaggaa gataaattca
      721 aagttgtgtt agatgatgaa tttacaaaag aagaggtaaa tggagctaag ggcattataa
      781 agactacaga taaagagaca ggtgaggtta tcgtatataa ttactttgat gagcttgaaa
      841 aataatgctt aaagattact ttgattgaat gaaaaggtaa ttgtgttgag aatattaaat
      901 gactgaataa aaacagttgt atcaaaaaca aacaataata gaatgtaaga atgattgtgt
      961 cgaaaatatt aaatgactga ataaaaaata gttgcgtcaa aaacaaacaa taatagaatg
     1021 taagaatgat tgtatcaaga atataaaatg attcaacaaa aaacgattgt gttaaaaaca
     1081 aacaacaata gaatgtaaga atgattgtat caagaatata aaatgactga ataaaaacga
     1141 ttgcgtcaaa aa
//
LOCUS       DAMJWP010000398         1122 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.405, whole genome shotgun sequence.
ACCESSION   DAMJWP010000398 DAMJWP010000000
VERSION     DAMJWP010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.405"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(53..865)
                     /locus_tag="PC700_003683"
     CDS             complement(53..865)
                     /locus_tag="PC700_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2582897.1"
                     /translation="MYLERPELYKQSEINFWDDEYISKQLLKAHLDTNFEGASRNFNF
                     IEDSVNWIVTVANPVNYPNLLDLGCGPGLYAEKFAQKGYKVTGIDFSKRSINYAQNRN
                     KETNLNINYLFQSYLNMNYNDEFDLATLIYCDYGALSTENRRLLMEKIYDSLKPGGKL
                     ILDVFTINKYNNFEEIKYWEINEDGGFWSNEKYMCLQDNCKYNDYNTLEQTLVITEKD
                     ENVFYVWNHYFSKESFLLEVENIGFKSVEFFSNVKGEAYSDDSMTMGLVLQK"
BASE COUNT          405 a          184 c           92 g          441 t
ORIGIN      
        1 tattttattg ttaaaggttt caagttttta tttattcatt ttaaaaaaat tattattttt
       61 gtaaaactaa tcccatagtc atactatcgt cagaatatgc ttcaccctta acattactaa
      121 aaaactcaac agacttaaat ccaatatttt caacttctaa taagaaactt tcttttgaaa
      181 aatagtgatt ccaaacataa aatacatttt catctttttc ggttattaca agagtttgtt
      241 ctaatgtatt ataatcattg tatttacaat tatcttgtag acacatatat ttttcattac
      301 tccaaaatcc tccatcttca tttatttccc aatatttaat ttcctcaaaa ttattgtatt
      361 tatttattgt aaatacatct aatattaatt ttcctccagg ttttaaacta tcataaattt
      421 tttccattaa taaccttcta ttttcagttg acaaagctcc ataatcacaa taaattaaag
      481 tagccaaatc aaattcatca ttatagttca tatttaaata actttgaaac aagtaattta
      541 tgtttaaatt agtctcctta tttctatttt gtgcatagtt aatagaacgt tttgagaaat
      601 caattccagt taccttataa cctttttgtg caaatttctc tgcatataaa cctggaccac
      661 aacctaaatc aagtaaatta ggataattaa ctggatttgc aactgtaact atccaattta
      721 cagaatcctc gataaagtta aaatttctac ttgctccttc aaaattagtg tctaaatgtg
      781 cttttaataa ttgttttgaa atatactcat catcccaaaa attaatttca ctttgtttat
      841 aaagctctgg tctttctaaa tacatacata atttgtttaa caaaacatac cactccttaa
      901 aatttatttt taaaataaaa aaaccacaga gtttactctg tggtttaact tcaaaaagat
      961 gagtttaaat aagagtaaaa tagaaccaaa tatttttaag cacaacaata aaactatagc
     1021 tttactttgt ttgcttatta aatttgattt tattcactct tttccagcaa tacttatatc
     1081 aacgatttta tgatatcaaa tatcaccgaa caacatcatc ct
//
LOCUS       DAMJWP010000399         1119 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.406, whole genome shotgun sequence.
ACCESSION   DAMJWP010000399 DAMJWP010000000
VERSION     DAMJWP010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.406"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            186..896
                     /gene="rgaR"
                     /locus_tag="PC700_003684"
     CDS             186..896
                     /gene="rgaR"
                     /locus_tag="PC700_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgaR"
                     /protein_id="HDF2582898.1"
                     /translation="MFRVVICDDEKIQRSILKEFTKKLLSERCLNYQILEFSCGEDLI
                     SKYPEKIDIIFLDIQMKDINGIEVAKKIRKFDEKVEIIFTTAFSEYAPQGYEVRAYRY
                     LVKPIEYSNFAMGVNLCIDNLQRKKERYIVLNSKKGFNRILIDSILYVETVKRDLVVH
                     TIGRDYKANMSMKQAEKLLCENGFFRCHTSFIVNLRRIEDLRDNMITINEQFIPISKY
                     RLKDLKVALTSLLCDIMY"
BASE COUNT          454 a           92 c          183 g          390 t
ORIGIN      
        1 ataatatatc tagaaaagta taggaattta tcgtaattta agtttaaata ttaaagagaa
       61 tttctataag tagtttaatc gacaacttaa atttataaag aaatttaaat ttgaaagcta
      121 gtgagtacag tttggaaatt gaaataaagt aaactaaatg atattttaga tttggaggtg
      181 atattatgtt cagggtagtt atatgtgatg acgaaaaaat acaaagaagc atactaaaag
      241 agtttacaaa aaaacttttg tctgaaagat gtctaaatta tcaaatactg gaattttcct
      301 gtggagaaga tttaatatcc aaatacccag aaaaaattga tattatattt ttagatatcc
      361 aaatgaaaga tatcaatgga attgaggtgg ctaaaaaaat aagaaagttt gatgaaaaag
      421 tagaaattat atttacaaca gctttttcag agtatgcccc ccaaggttat gaggttcgtg
      481 cttatagata tttagtaaag cctatagagt atagtaactt tgcaatgggt gtaaatcttt
      541 gtattgataa ccttcaaaga aagaaggagc gttatatagt tttaaatagt aaaaaaggat
      601 ttaatagaat actgatagat tctattttat atgttgaaac tgtaaagaga gatttagttg
      661 tacatacaat aggtagagat tataaagcta atatgagcat gaaacaagca gaaaaattac
      721 tgtgtgaaaa tggctttttt agatgtcata caagttttat tgttaattta agaagaatag
      781 aagatcttag ggataatatg attactatta atgaacaatt tatacctata agtaagtata
      841 gacttaaaga tttaaaagta gcattaacaa gcttgttatg tgatataatg tattaatttt
      901 aaaaaggtaa ttgaaaatat cgtaaaaaag atttaatgtc atcacagggc tattgattaa
      961 aactgtctta atatataaaa tgttatttat attaagacag ttttattatg caaaatatca
     1021 gtttatagac ttttaatatt gtttaatatg tatatgtaga gtttccatta cagtagcatg
     1081 attattaatt agtagcctga ttattaatat actattatt
//
LOCUS       DAMJWP010000400         1112 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.407, whole genome shotgun sequence.
ACCESSION   DAMJWP010000400 DAMJWP010000000
VERSION     DAMJWP010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.407"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            25..804
                     /locus_tag="PC700_003685"
     CDS             25..804
                     /locus_tag="PC700_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582899.1"
                     /translation="MKVNENNINNIDEELDELSRKVYLESPDFWISISDKIIDKVFDE
                     MLLFFAVKYNYFNIVCFAIENNYLDLNMPSKNKKYTNIMEHLLDTSKQNEDKKIYNYL
                     LSLNKGNNLDERPILDELNCNISSDTFEKKDIYLPKYLCPSCNSNIFDTGYKVSNDIT
                     YKFSSKEAEPIEMSSEVSSIRCCNCDELLEDITLNKLYNLCKIQNCNNCGSNLTKVGI
                     IAKKKMNFDENTEKFKDVSTSYCCGNCDSEINLSQREHFKI"
BASE COUNT          462 a          113 c          140 g          397 t
ORIGIN      
        1 agatatttaa aaggaagtga tgatatgaaa gttaatgaaa ataatataaa taatatagat
       61 gaagagttag atgaacttag taggaaagtt tacttagagt caccagattt ttggataagt
      121 atttctgaca aaataataga taaagtattt gatgaaatgt tattattttt tgcagttaaa
      181 tataactatt tcaatatagt atgctttgct attgaaaaca attatctgga tttaaacatg
      241 ccttcaaaaa acaaaaaata cactaatata atggagcatt tgttagatac atcaaaacag
      301 aatgaagata aaaaaattta taattatctt ctaagcttaa ataaaggtaa taacctagat
      361 gaaagaccaa ttttagatga attaaactgt aatattagta gtgatacttt tgaaaaaaaa
      421 gatatatatc ttccaaagta cttatgtcca agttgtaatt ctaatatatt tgatacaggt
      481 tataaagtat caaatgacat aacatataaa ttttcttcta aagaagctga acctattgaa
      541 atgtctagtg aagtttcttc tatcagatgt tgtaattgtg atgaattatt agaagatatt
      601 acacttaata aactatataa cctatgtaaa attcaaaact gtaataattg tggttctaat
      661 ttaactaagg taggaattat tgcaaagaaa aaaatgaact ttgatgagaa tactgaaaaa
      721 tttaaagatg tctcaacatc ttactgttgt ggaaattgtg actctgaaat aaacttatct
      781 caaagagaac attttaagat ttaaatattt ataggctaaa gtaataagat tacattatta
      841 gatactgtat aagtttttta aatctgtaaa aataaataca cttataagaa ttaaaattgc
      901 tatgaaacta gttcttgctg aactaatttc atggcaattt tctataatct aataattatt
      961 aaattttaac aaacctttat tagcattcca taattattca aaattctttc aaatacccta
     1021 tagtttatag gtgaaagtat ctttaatatt atatcaaact tattatcttt aaatagtaaa
     1081 tttgttaaat ctgaaataca ccatacaatt tt
//
LOCUS       DAMJWP010000401         1111 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.408, whole genome shotgun sequence.
ACCESSION   DAMJWP010000401 DAMJWP010000000
VERSION     DAMJWP010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.408"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            240..941
                     /locus_tag="PC700_003686"
     CDS             240..941
                     /locus_tag="PC700_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HDF2582900.1"
                     /translation="MSIEDLYDFECVPLNNEIDIDVLFEKMIEDGKEKEYTPIIVIDE
                     KVGLLRKNIDMITKEYGSLENYREYCLTKYNEIDVNQFFDFRKSEISELLKELIDLED
                     DFYDYKHFRPIINIDIFHEYNKVYIAKIPTIKPYEVFAYIPIGGFNDCPRDEEHIAIA
                     KYWYEKHGIFPIAIGCDTVQYSVKNLTKDGKKFDDLCIELVFYCEDIIIQGYETLKAL
                     KDVLKRSTIVLFWWD"
BASE COUNT          440 a           95 c          172 g          404 t
ORIGIN      
        1 ttacttatta ttaaccatga acatttgttt tgtgctatca tatttatata agggaattgt
       61 cataaaaaat atcttgttca tacagtttta tatagaaaaa agaaatgaat cttttcttta
      121 tataagtata ttttgtaaat tgcctctata tttatagttt aaataatagt ggttttaaaa
      181 atctgacaac aaaaataaaa ctttcaaaaa attaaatcta aaaagaatag gagataaata
      241 tgagtattga agatttatat gattttgaat gtgttccttt gaacaatgag attgatattg
      301 atgttttatt tgaaaagatg atagaagatg gaaaagaaaa agaatataca ccaattatag
      361 tcattgatga aaaagtaggg ctattaagaa aaaatataga tatgattact aaagagtatg
      421 gttctcttga aaactataga gagtattgtt taacaaaata taatgaaatt gatgtaaatc
      481 aattttttga ttttaggaag agtgagattt cagaattatt aaaggagcta attgatttag
      541 aagatgattt ttatgattat aaacatttta gaccaattat aaatatagat atatttcatg
      601 aatataacaa agtttacata gctaaaattc caacaattaa accatatgaa gtatttgcat
      661 acatacctat tggaggattt aatgattgtc ctagagatga agaacatata gctattgcaa
      721 aatattggta tgaaaagcat ggaatctttc caatcgcaat tggatgtgat acagttcaat
      781 attctgtaaa aaatttaact aaagatggta aaaaatttga tgacttatgt attgaattag
      841 tattttattg tgaggatatt attatacaag ggtatgaaac cttaaaggct cttaaggatg
      901 tattaaaacg ctctacaata gtgctttttt ggtgggattg atattctatc atttagatag
      961 tattaaattg ttagtcacca agaatccact tactttagtt atggggagtg tgtcaaacta
     1021 ggtttttata cacagtgtag catagtaata tgtttaaatt acaaaggtta aaaaaatact
     1081 ttaatatttt taatattata attaatagat t
//
LOCUS       DAMJWP010000402         1108 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.409, whole genome shotgun sequence.
ACCESSION   DAMJWP010000402 DAMJWP010000000
VERSION     DAMJWP010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.409"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(195..1064)
                     /locus_tag="PC700_003687"
                     /pseudo
     CDS             complement(195..1064)
                     /locus_tag="PC700_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
BASE COUNT          414 a          171 c          107 g          416 t
ORIGIN      
        1 aataaactaa aaaatatcga aaaataattt aaaagacatc aaaactaaat cttctaatca
       61 acaattaaaa gtctattttc ttagtttaaa aaataaagat agattttttc aaaacactgg
      121 ataaataggt aaaaaaataa acagttagta aaataaattt ttactaactg tttattttta
      181 ttgtttaata tgtcctataa atcctctaag taatattttc cttgcaaaaa ctctgattct
      241 aaacctagat ttttaaaata atcaaccatg cccatacacg caaatttttc tgtagaataa
      301 tgagtagcac ctataacatt aattaaatta tcttttgcta tttgatggaa ctctaatact
      361 ggttcaaaat gttttaaagg cttagtaaac ccagtaatat atagatttat accaagttca
      421 gcaacttctc ttgctacaaa tggataactt cctccccctg ctgcaatagc aactaaacca
      481 tcttttaata cagaatctcc ataatcatac aatttcacat catgaccaac agatttttta
      541 attaattcct taatctcttc tattgatttt agtgatgttt tacatataac tcctacctca
      601 attttatcgt catatttaca aaatggtttg actatttcta aacccatagc ttttgcaaaa
      661 ctaacagaag ttgaatagtc actatagttg tctagtggag aatgtaatac ataaaaagaa
      721 attctacgat ttttcatctc ttccatataa gatagtggta tgtcgtgaaa tggaaaacct
      781 tcatctgatg ctatgtatcc cattgcgtga tgtgaaaaaa ttaaaatatc tttttcatct
      841 ttatctataa cttgcttaat tatttcttta tcaggaaaag tagttgtgta tactttatta
      901 atattttgag cattatctaa tactaatcat atgtactttt ttctaaattc tggagtgata
      961 aaatcatttt caaaattcat aaaactccaa tcatcattaa tatttttaat attaaattct
     1021 aaatctaatt ttttataaag ttcaattgcc ttaataataa ccactccttt ataataatat
     1081 tgaattacta catattaata tagaaatt
//
LOCUS       DAMJWP010000403         1076 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.410, whole genome shotgun sequence.
ACCESSION   DAMJWP010000403 DAMJWP010000000
VERSION     DAMJWP010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.410"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..245
                     /locus_tag="PC700_003688"
     CDS             <1..245
                     /locus_tag="PC700_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582901.1"
                     /translation="SQDKTNLMQINQIKIDGSQVYAVFESKIDTVKIGDYENNTRAIQ
                     VLDNIQNFIENGTKYDYITSNKVRYNVNKIFQMPAK"
     gene            complement(355..1035)
                     /locus_tag="PC700_003689"
     CDS             complement(355..1035)
                     /locus_tag="PC700_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDF2582902.1"
                     /translation="MIWTFYSFICVILPCLIYQIVVIKRKNLKVEKNVMIHLVWVYIF
                     LLYIYLALSKAGIGSIWDIGRYSGLFRIDEINLIPFDSVGILTYILNIIMFMPLGFLL
                     PLIWKNFRNIINVSLTGLGFSLAIELCQLFNLRATDIDDLMMNTLGAVLGYFIWVGVN
                     NLFNLIKKKDIFPSIYKNIIQDEIAITIDDDISKKITSLYKNEAIIYLILAVLGEFLL
                     FNWSIIPY"
BASE COUNT          434 a          141 c          123 g          378 t
ORIGIN      
        1 gaagtcaaga taaaactaat ttaatgcaga taaaccaaat taaaatagat ggtagtcaag
       61 tttatgcagt atttgaaagt aaaattgata ctgtaaaaat aggagattat gaaaataata
      121 caagagctat acaagtgttg gacaatatac aaaacttcat tgaaaatgga acaaaatatg
      181 attatataac atcaaataaa gtcagataca atgttaataa aatatttcag atgccagcta
      241 aatagtgagt gtgcatttta gtttaataac taaagcccac tttcttttgt agtatgaaag
      301 agggctttac atatttgtaa atatcgaagt acacttgttt tatcaataaa ttatttaata
      361 aggtataatg gaccaattaa acaataaaaa ttctcctaaa acagcaagta ttaaatatat
      421 aatagcttca tttttgtaaa gagaggtaat ttttttgctt atgtcatcat caattgtaat
      481 agcaatttca tcttgaataa tatttttata tatagatggg aaaatatctt ttttctttat
      541 aagattaaat aaattattta cacctaccca tataaaatat cctaaaacag cacctaatgt
      601 gttcatcatt aaatcatcaa tatctgtagc tcttaagtta aacaattgac aaagttcaat
      661 tgctaatgaa aaacctaatc cagttaatga aacattgata atatttctaa aatttttcca
      721 aattaaaggt agtaaaaatc ctaaaggcat aaacataatt atatttaaaa tatatgttaa
      781 gatgccaact gagtcaaaag gtattagatt tatttcatca attctaaaaa gcccactata
      841 ccttcctata tcccatatgc taccaattcc agctttacta agtgctagat agatatataa
      901 taaaaaaata tatacccata ctaaatgtat cattacattc ttttcaactt tcaaattttt
      961 tcgttttata acgactattt ggtatattaa gcatggtaat ataacgcaaa taaatgaata
     1021 aaatgtccat atcattttat tttgtcccct ttcaaattta tgtttttagt tttata
//
LOCUS       DAMJWP010000404         1048 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.411, whole genome shotgun sequence.
ACCESSION   DAMJWP010000404 DAMJWP010000000
VERSION     DAMJWP010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.411"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..548
                     /locus_tag="PC700_003690"
     CDS             <1..548
                     /locus_tag="PC700_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HDF2582903.1"
                     /translation="SHINILIIADINNKDICLKLNIKYMFNLININRQLYPAENSKNN
                     DKKEGMKNNIDSSILLADDLLSIYRLLKKIKIHELYSNINFGTGNIGLTSSVYVLINT
                     LYGNLFNMIDAEKMYLNVNPDFTKDYVLGNIRIHIRPRIKALFNIIIMINKIMNKNKG
                     NKEGDSNESNRFDTKSYGNNS"
     gene            502..930
                     /gene="ytfJ"
                     /locus_tag="PC700_003691"
     CDS             502..930
                     /gene="ytfJ"
                     /locus_tag="PC700_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HDF2582904.1"
                     /translation="MRATGSIQSLMETTLETIKGSIDANTIIGDPIKTDTTVVVPISK
                     VTIGFGIGGGEYSKGYDDKDREIELKNEKSDTNFAGGSAGAISVQPVAFVVVESGETR
                     IMSLDSNINLVDNILSITPRVLEKIQNISQSNKNQDKNNM"
BASE COUNT          451 a           93 c          170 g          334 t
ORIGIN      
        1 tatctcatat aaacatctta ataatagctg atataaacaa caaagatatt tgtttaaagc
       61 ttaatataaa atacatgttt aatttaataa atataaatag acaactttat ccagctgaaa
      121 atagtaaaaa taatgataaa aaagagggga tgaagaataa tatagattca agtatattat
      181 tagctgatga ccttttgagc atttatagat tattaaaaaa gataaaaata catgaacttt
      241 attcaaatat taattttgga actggaaata ttggcttaac atcttctgta tatgtattga
      301 taaatacttt gtatggaaat ttatttaata tgattgatgc agaaaaaatg tatttaaatg
      361 taaatccaga ttttacaaaa gattatgttt taggaaatat aaggatacat ataaggccaa
      421 gaataaaagc tttatttaat attataataa tgattaataa aattatgaat aaaaataaag
      481 gaaataagga aggtgatagt aatgagagca acaggttcga tacaaagtct tatggaaaca
      541 actcttgaaa caattaaagg ttctatagat gcaaatacta ttataggaga ccctataaaa
      601 acagatacta cagtggttgt acctatatca aaggttacaa ttggctttgg tataggagga
      661 ggagaatact caaaaggata tgatgataaa gatagggaaa ttgaattaaa aaatgaaaaa
      721 tcagatacga attttgctgg aggtagcgca ggggctatat ctgtacaacc agtagcattt
      781 gtagtagttg aaagtggaga gactagaata atgagtttag acagtaatat caatctagtg
      841 gataatatat tatctataac tcctagagta ttggagaaaa ttcaaaatat atcacaaagt
      901 aataagaatc aagataaaaa caatatgtaa gaataaaata tttttaaata tgtatatagt
      961 tttaagtaaa gtagtggggt tagtataaat cccactattt tgttgtgtac tttaaacatt
     1021 tatatctaat aagattttag tttaaaac
//
LOCUS       DAMJWP010000405         1041 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.412, whole genome shotgun sequence.
ACCESSION   DAMJWP010000405 DAMJWP010000000
VERSION     DAMJWP010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.412"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(200..691)
                     /locus_tag="PC700_003692"
     CDS             complement(200..691)
                     /locus_tag="PC700_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HDF2582905.1"
                     /translation="MGMIGCYTKISEENVFKLQQAEENLQDFVFEDANENSTINIDKA
                     WHAIHFTLTGCPFGGDDENIFSKLVLSGNILMEIDGEFPVMLITANDVKKLSKAMNSL
                     EEQAFRKRFNINEMLENNIYPVMNDESEDDFFEYVWANLIELKKFIQEASDDGLAVIF
                     FIM"
BASE COUNT          395 a          156 c           87 g          403 t
ORIGIN      
        1 tatattgcaa aaatttaaaa ctaatttata tatgctaata taaatattta taaagacata
       61 agacaaattt acctacagat ttaaatttaa cttatatatt ttaaaaggct aatataatat
      121 gtattttctt ctatacaatc aaaaggcttc atatctaaga tatgttatga cactaatatt
      181 cacaattttt tagtaattat cacattataa aaaatattac agctagtcca tcatcagatg
      241 cttcttgtat aaacttttta agctcaatta gatttgccca tacatattca aaaaaatcat
      301 cttcactctc atcattcata acaggataaa tattattttc taacatctca ttaatattaa
      361 atctttttct aaatgcttgt tcttccaaac tgttcattgc ttttgatagt ttttttacat
      421 catttgctgt aatcaacatg acaggaaatt ctccatcaat ttccataagt atgtttccac
      481 ttagtactaa tttgctaaat atattctcat catctccacc aaaaggacaa cctgtcaaag
      541 taaagtgaat ggcatgccat gctttatcaa tattgatagt actattttcg ttggcatctt
      601 caaaaacaaa atcttgtaaa ttttcttctg cttgctgtaa tttaaaaaca ttctcttcac
      661 ttattttagt ataacatcct atcatcccca taattacatt cctcctaagt agcgtttttt
      721 taattgtaac ttataattaa acttagttac aataaaatat attgagaaaa gtattatttc
      781 atcttaaatt taaataccta tattatcaac taaaattctg tgtttatagc gtaagtatta
      841 ttattttttc ctaaaaatta aatgaatcag aagttctata ggcaatattg actataaata
      901 agaactatta cttttaaaaa aaatttaaga aattatactg tagaatcatg ctctacttat
      961 tagcttcaaa taaaaaataa atcatgttct caaattaaat aataaataac aataaattta
     1021 aattttaaac tataagagtt t
//
LOCUS       DAMJWP010000406         1014 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.413, whole genome shotgun sequence.
ACCESSION   DAMJWP010000406 DAMJWP010000000
VERSION     DAMJWP010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.413"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            59..940
                     /locus_tag="PC700_003693"
     CDS             59..940
                     /locus_tag="PC700_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HDF2582906.1"
                     /translation="MKKRLIIMILSVVLVLSSILTIFAYSNIKYNNNKLIYSNMIDKK
                     TQNSVKEILKENKINEKDIDTFIKAVNNYNKLQVKILQNNINISKSGYSSINAKQVPY
                     NLEKLQDNWVKKFPDYMDVNCRITAFRLFKDFINSNKKFTGDSIDLNVDLDTIMNNKD
                     AKFSTKDVEKFINFFSAIPAKDTDDTIKIAEQIKNEWKKRKISFKNNKNISIINGFLR
                     YPETKNVFIGHTGICIKTKNGILFLEKYGVTSPYQVTKFKNKKGVKNYMFNRLKMSEG
                     EIELPDPIIMENDKLMK"
BASE COUNT          467 a          104 c          125 g          318 t
ORIGIN      
        1 aaaggtacta tatactataa atatgtcgac acaataaatt taaaaaggag aactaaatat
       61 gaaaaaaaga ttaataataa tgatattgtc tgttgtctta gtactatcaa gtatacttac
      121 gatatttgca tattcaaata ttaaatataa taataataaa ttgatatatt ctaatatgat
      181 agataaaaaa actcaaaata gtgtaaagga aatcctaaaa gaaaataaaa ttaatgaaaa
      241 agacatagat acatttatta aagcagtcaa caattataat aaattgcaag ttaaaatact
      301 tcaaaataat ataaatatat ctaaatctgg atattcttca atcaatgcaa aacaagttcc
      361 atataaccta gaaaaattgc aagataattg ggtgaaaaaa ttccctgatt atatggatgt
      421 aaattgtaga ataactgctt ttagactttt taaagatttt ataaattcaa ataagaaatt
      481 tactggagac tctattgatt tgaatgtcga cttagacact ataatgaata ataaagatgc
      541 taaatttagc accaaagatg tagaaaaatt tatcaacttc ttttctgcaa taccagctaa
      601 agatacagat gatacaatca aaattgcaga acaaataaaa aatgaatgga aaaaaagaaa
      661 aatatctttt aaaaataata aaaatatatc tattataaat ggatttcttc gttatccaga
      721 aactaaaaac gtatttatag gtcatacagg catatgtatt aaaactaaaa atggcattct
      781 atttttagaa aaatatggtg ttacatcacc ttatcaagtt actaaattta aaaataaaaa
      841 aggtgtaaaa aattatatgt ttaatagatt gaagatgtca gaaggtgaaa ttgaattgcc
      901 tgaccctata ataatggaaa acgataaact tatgaaataa taactttaaa cgcaaaatag
      961 tatttataat tactaagtca atataataga gaaggtctat agatttgtgt aaat
//
LOCUS       DAMJWP010000407         1006 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.414, whole genome shotgun sequence.
ACCESSION   DAMJWP010000407 DAMJWP010000000
VERSION     DAMJWP010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.414"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(281..955)
                     /locus_tag="PC700_003694"
     CDS             complement(281..955)
                     /locus_tag="PC700_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2582907.1"
                     /translation="MFKIMVVEDDVSLKNIIAKCLTKWGHDVHQIENLENIIEEFKNY
                     NPELVLLDINLPFYDGFHWCNEIRKISKVPIIFISSRNSNMDVIMGVNLGADDYIQKP
                     FSVDVLVAKVNALLRRTYNFVDNNSNQIIHNGVTLDLSTATINYEDNTIELTKNEIKI
                     LHELMKYKGQIVSRNKLMKKLWDNDWFVDDNTLTVNVNRIRSKLNEIGLEDFIETKRG
                     LGYIIS"
BASE COUNT          364 a          155 c           95 g          392 t
ORIGIN      
        1 ctagctaatt ctatatcatt catattaatt ttgaatgtat cattttttat cttatctaaa
       61 aatttgttct tcttgatgta gctaataaat agatataaaa ataaaaacac aacatttaca
      121 actaatatat ataaaatagt atcagtcaga aaaacatcta caggagaaaa tataataact
      181 gatactgtaa aaattaagaa agtaaaattg tatactaaaa aacctagttt atccttgaga
      241 aaatctttaa agctcataaa ttcacctact tagaattgtt ttaactgatt atataaccca
      301 atcctctttt tgtttctata aaatcttcta aaccaatttc atttaatttt gagcgtattc
      361 tatttacatt gacagttaat gtattatcat ctacaaacca atcattatcc cataattttt
      421 tcatgagttt attcctactt actatctgcc ccttatattt cataagctca tgaagaattt
      481 tgatttcatt ttttgttagc tcaatagtat tatcttcata gtttattgtt gcagttgata
      541 aatctaaagt aacaccattg tgaataatct gatttgagtt attatcaaca aaattataag
      601 ttcttcttaa aagggcatta actttagcta ctaagacatc tacagaaaaa ggtttttgta
      661 tataatcatc tgcacctaaa tttacaccca ttattacatc catattagaa tttcttgaag
      721 aaataaaaat tataggaact tttgaaattt ttcttatttc attacaccaa tgaaaaccat
      781 cataaaatgg aaggtttatg tccaataaaa ctaattctgg gttatagttt ttgaactctt
      841 ctattatatt ttcaagattt tctatttgat gaacatcatg tccccattta gtaagacact
      901 ttgctatgat atttttcaat gagacgtcat cttcaactac cattatttta aacattctta
      961 cctcctaatt caaaatagtt atattaacat aactatattc tacttg
//
LOCUS       DAMJWP010000408          988 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.415, whole genome shotgun sequence.
ACCESSION   DAMJWP010000408 DAMJWP010000000
VERSION     DAMJWP010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.415"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            47..802
                     /locus_tag="PC700_003695"
     CDS             47..802
                     /locus_tag="PC700_003695"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDF2582908.1"
                     /translation="MLKVIINGYNGTMGRVLAECVKKDNELELVCGVARSADEKGHHS
                     DTKLYKTMTDVTEKTDVIIDFSHHETLEPILSYALKNNTPVVLATTGYDENDLNKIEE
                     ASKNIPILLSSNTSLGVNILIKLVKDASKMLEGFDIEIIEKHHNRKEDAPSGTATMIA
                     NAIREVLPESENTHGRYGRDCKRKPNEIGIHSIRGGNIISDHDVIFAGDNEVLTLSHH
                     SQSDEVFANGSIVAAKFLIGKQSGLYKMSDILG"
BASE COUNT          378 a          114 c          162 g          334 t
ORIGIN      
        1 cttatagaaa tataacaaat ttttattaat ttcataggag gaatttatat tgaaagttat
       61 aattaatggc tataatggaa caatgggaag agttttagca gaatgtgtaa aaaaagataa
      121 tgaattagag ttggtttgtg gtgtagctag gtctgctgat gaaaaaggtc atcatagtga
      181 tacaaagctt tacaaaacaa tgactgatgt aactgaaaaa actgatgtca taatagactt
      241 ttcacatcat gagactttag agccaatatt gtcatatgct ctaaaaaata atacacctgt
      301 agtattagct actactggat atgatgaaaa tgacttaaat aaaatagaag aagcatcaaa
      361 aaacattcct atattattat catcaaatac atctcttgga gtgaatatat taataaaatt
      421 agttaaggat gcttcaaaga tgttagaagg ttttgatatt gaaatcatag aaaaacatca
      481 caatagaaaa gaagacgccc ctagtggaac tgctactatg atagctaatg caattagaga
      541 agtcttgcct gaaagtgaaa atacacatgg tagatatggt agagattgta aaagaaaacc
      601 aaatgaaatt ggaatacact ctataagagg tggcaatata atcagcgacc atgatgtcat
      661 atttgcaggt gataatgaag ttcttacatt aagccatcat tctcaatctg atgaagtatt
      721 tgcaaacggt tctatagttg cagctaaatt tttaattggt aagcaatctg gactttataa
      781 aatgtcagat atactaggat aagttttgta cataataaat ctcaatctat ttaggtattc
      841 atattcatca atttgaatat ctaattgatt gagatttcta ttctaaaagt ttataaattt
      901 tatgcttacc ttttaaatat aatcattttt cttaaagatt atcttattta ttggtatatg
      961 gaattatatt gtttattatt ttatagtc
//
LOCUS       DAMJWP010000409          982 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.416, whole genome shotgun sequence.
ACCESSION   DAMJWP010000409 DAMJWP010000000
VERSION     DAMJWP010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.416"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            48..800
                     /gene="cwlD"
                     /locus_tag="PC700_003696"
     CDS             48..800
                     /gene="cwlD"
                     /locus_tag="PC700_003696"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase CwlD"
                     /protein_id="HDF2582909.1"
                     /translation="MEYYSVFISIHIQGDVVRKYIKHIIFSFAMICLVVVSIFEIKNI
                     SEDVIKYMPVTNKTIILDAGHGGIDPGALNKDKSTSEKDINLAITLKLRELIESSGGL
                     VILTREDDSSLYKEENNKTTRQKYNENLKNRKEIISNSNANMFVSIHLNAFEQSKYYG
                     AQTFYPKDKQDSKELSKCIQEELKRVVDKTNNREVKPRDDIYLLKENNIPSVLIECGF
                     LSNEKECKLLTDETYQEKIAWAIYIGIQKYLS"
BASE COUNT          432 a          102 c          154 g          294 t
ORIGIN      
        1 ataaagatat atcaatatta aaaaaattta aatgtttgta ctaatatatg gaatattata
       61 gtgtttttat aagtatacat atacagggtg atgttgtgag aaagtacata aaacatataa
      121 tttttagttt tgcaatgata tgtctagtag tagtatccat atttgaaatt aaaaatattt
      181 ctgaagatgt tatcaagtat atgccagtaa caaacaaaac tataatttta gatgctggtc
      241 atggaggcat tgatccaggt gcattaaata aggataagag tacatctgaa aaggatatta
      301 acctagcaat aacacttaag cttagagagc ttatagaatc aagtggtggc cttgtaatat
      361 taactcgaga agatgacagt agcctttata aagaagaaaa taataaaact acaagacaaa
      421 aatataatga gaatttgaaa aatagaaaag aaataatatc taattctaat gccaatatgt
      481 ttgtctctat acatcttaat gcatttgaac aatcaaaata ctatggagct caaacttttt
      541 atcctaaaga caaacaagac agtaaagaat tatcgaagtg tattcaagaa gaacttaaaa
      601 gagtagtaga taagacaaac aacagagaag tcaaaccaag ggatgatata tatcttttga
      661 aagaaaataa tattccatca gtactgatag aatgtggttt tttatcaaat gaaaaagagt
      721 gtaaactctt aactgatgaa acatatcaag aaaaaatagc atgggcaatc tacataggaa
      781 tacaaaaata tttaagttga aataagtata aaaataggtc taaatatgta aatagcttgt
      841 attaaagcag ttaaaatgca ttaatatagg ctatttttat tttgacaaaa aaatatttaa
      901 aataaaagtt aaaaagttgt tgacttagaa taatatagat gatattatat atgagtgccc
      961 aaaaggagca ccaaaataag ac
//
LOCUS       DAMJWP010000410          982 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.417, whole genome shotgun sequence.
ACCESSION   DAMJWP010000410 DAMJWP010000000
VERSION     DAMJWP010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.417"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..>982
                     /locus_tag="PC700_003697"
     CDS             <1..>982
                     /locus_tag="PC700_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2582910.1"
                     /translation="LATSIDISNDKVFEEFLYKHISSLCKKIKLGINQSPIMAQDIKI
                     KYPFAFELANIAKKTIEKDLKIEINEDEVANIALHIGGAVERASYNNKDKVFKTIIVC
                     TSGIGTSMLIKAKLENIFKEKLEILKIIPSYLVEYIKVIDVDFVISTVPIEVGDVPVI
                     NISPILSEKEIRLIEKYIETGNIYLDLDIKSLFDSELFFTDLVFKRKEEVIDFMASKL
                     VQKGYIDEDMKKSYFEREKIATTEIGNMVTIPHGAIGKIFDNKIAIGILKKPISWEIS
                     DVRLIIMLALDKDKILDYEVIFSKIYKRVDSIAKVISICENSSFEKFVNLF"
BASE COUNT          423 a           80 c          162 g          317 t
ORIGIN      
        1 attagctaca tcaatagata taagtaatga taaggtattt gaagagtttt tatataagca
       61 tattagtagt ttatgtaaaa aaataaagtt aggtataaat caatcaccta taatggctca
      121 agatataaaa ataaaatatc catttgcttt tgaactagct aatatagcta aaaagacaat
      181 agaaaaagat ttgaagatag aaattaatga agatgaagtt gcaaacatag ctctacatat
      241 aggaggagca gttgaaagag catcttataa taataaagat aaggttttca aaactataat
      301 agtttgtact tcaggcatag gcacatcaat gttaataaaa gctaaattag agaatatatt
      361 taaggaaaaa ttagaaatac taaagattat tccttcatac ttagttgaat atataaaagt
      421 tatagatgtt gattttgtaa tatctactgt tcctattgaa gtaggagatg tgccagtaat
      481 aaatatatct ccaattttaa gtgagaaaga aattagatta attgaaaaat atatagaaac
      541 aggaaatata tacctagatt tagatataaa aagcctgttt gatagtgaat tattttttac
      601 tgatttggtg tttaaaagga aagaagaagt tatagatttt atggcaagta aacttgtaca
      661 aaagggctat atagatgagg atatgaaaaa atcttacttt gagagagaaa aaatagcaac
      721 aacagaaata gggaatatgg taactatacc acatggagct attggaaaaa tttttgataa
      781 taaaatagca attggtatat taaagaagcc tatttcttgg gaaatatcag atgttcgttt
      841 aataataatg ttagcccttg ataaagataa aatattagat tatgaagtaa tattttcaaa
      901 aatatataaa agagtagatt ctatagcaaa agttataagt atatgtgaaa atagtagttt
      961 tgaaaaattt gtaaatttat tt
//
LOCUS       DAMJWP010000411          970 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.418, whole genome shotgun sequence.
ACCESSION   DAMJWP010000411 DAMJWP010000000
VERSION     DAMJWP010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..970
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.418"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(164..874)
                     /locus_tag="PC700_003698"
     CDS             complement(164..874)
                     /locus_tag="PC700_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2582911.1"
                     /translation="MTSICICDDEKQQRNMLNKIITREMDLWGYPYQIKEYSNGESLV
                     YNLEHENYYIDIIFLDIELEKSNGVDIAKTIRKHSKDTIIIFVTGFSDYVFHGYDVGA
                     LNYILKPYKTEKICKVLKEALKKLEQWKENFITVHIGTNLCKINTKDILYFKSELRKL
                     TVVTFDKTLEYYGKLNDMETVLPSNFIRTHQRYIVNLNHVDSITQTYAKVQNEKIPIS
                     RKKYQEVVGKFTRYMLEP"
BASE COUNT          337 a          133 c          114 g          386 t
ORIGIN      
        1 caaaaaaact ctaaaaacta agcttcaaaa aattaaattt tgaaagattg aaattctaga
       61 aattaagctt caaaaaatta aatttgaaaa aacaaaactc taaaaactga gctttaaaaa
      121 attaagtttc aaaaataaaa ttctagagat tgaacttcaa aaattaaggt tctaacatat
      181 accttgtaaa ttttcctaca acctcttgat attttttacg gcttatagga attttttcat
      241 tctgaacttt ggcataagtt tgagttatac tatctacatg gtttagattg acaatatacc
      301 tttgatgcgt acgtataaaa ttagagggaa gaacagtttc catatcattt aattttccgt
      361 agtattctaa tgttttatca aaggtaacta cagttaattt tcttagctcg cttttaaagt
      421 agaggatatc ttttgtatta attttacaaa gattagttcc aatatgaact gtaataaagt
      481 tttctttcca ttgttctaat ttttttagtg cttcttttaa tactttacat attttttcag
      541 ttttataagg ttttaagatg tagttcaaag ctcctacatc atacccatga aatacatagt
      601 cagaaaaacc tgtgacaaag atgattatag tatcttttga atgttttcgt atagtttttg
      661 ctatgtcaac gccattactt ttttcaagtt caatatctaa aaaaataata tctatgtaat
      721 aattttcgtg ttctagattg tatactagac tttctccatt tgaatattct tttatttggt
      781 aggggtagcc ccacaaatcc atttctcttg taatgatttt attcagcatg tttctttgtt
      841 gtttttcatc atcacaaata caaatcgaag tcaatttttt atctcctttc aagttttata
      901 tttatatagt taagtatata atatattttg ctacctagat atataaaatg aaataacaat
      961 tatatccaga
//
LOCUS       DAMJWP010000412          946 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.419, whole genome shotgun sequence.
ACCESSION   DAMJWP010000412 DAMJWP010000000
VERSION     DAMJWP010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..946
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.419"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(<1..845)
                     /locus_tag="PC700_003699"
     CDS             complement(<1..845)
                     /locus_tag="PC700_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HDF2582912.1"
                     /translation="MGVIIIQIRRPLLIILMFVVAISFIITNNTKNDEELNDKIITIQ
                     GVVKGKIEKKRYNQYKVGVFLINDYTKNKNLKIGQKVNITGKFKSLDKMKYDDFDYGR
                     YIKSTGYKGIVYINNYKIIDKNKLYSLIGEIKFYISKTYRYLYKKNSDFINSILLAEV
                     ENLTEEQKEIFTRTGTSHVISISGLHTGILCVIISFLLRGINKLYKLLILSIFITLYC
                     IMVGASPSIIRSIAFVMIFYLSIFIDRKKDGISALSLIGIILIMNNPYVIYNVSFQLS
                     FLATLS"
BASE COUNT          350 a          134 c           82 g          380 t
ORIGIN      
        1 gaaagtgtag ctaaaaaaga caattgaaaa cttacattat atataacata ggggttattc
       61 ataattaaaa ttatcccaat tagagacaat gctgatattc catctttctt tctatcaata
      121 aatattgata aataaaaaat cataacaaat gctatagacc taattatgga tggagacgca
      181 cctaccatta tacaatataa agttataaaa atcgacagta ttaataattt atacaattta
      241 tttatacctc ttaatagaaa ggatataatt acacataata ttcctgtatg aagacctgat
      301 atagatatta catgactagt tccagttcta gtaaaaattt ctttttgttc ctcagtcaaa
      361 ttttcaactt ctgctaatag tatagaattt ataaaatctg agtttttctt atataagtac
      421 ctataagttt tactaatata aaactttatt tcccctatta aagagtaaag tttattttta
      481 tcaataattt tatagttatt tatatacact atacctttat atccagtact ttttatatac
      541 cttccataat caaaatcatc atacttcatt ttatctaaac ttttgaattt acctgtaata
      601 ttaactttct gaccaatctt caaattttta ttttttgtat aatcatttat tagaaaaacc
      661 cctaccttgt actgattgta tcttttcttt tcaatttttc ctttgacaac cccttgtata
      721 gttattatct tatcattaag ttcttcgtcg ttttttgtgt tatttgtaat gataaatgat
      781 atagctacca caaacattaa tataattaat aatggtcttc ttatttgaat tataatcact
      841 cccatataaa agtatattat ttagattatt ttcaaataga aaatttttaa acaagataaa
      901 ttgttatcta aacaaaaaaa tacgtcaaaa gatttttaaa atcttt
//
LOCUS       DAMJWP010000413          938 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.420, whole genome shotgun sequence.
ACCESSION   DAMJWP010000413 DAMJWP010000000
VERSION     DAMJWP010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..938
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.420"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(231..698)
                     /locus_tag="PC700_003700"
     CDS             complement(231..698)
                     /locus_tag="PC700_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2582913.1"
                     /translation="MKFLNVLIVVEDIEKSKKFYYDILGLKVICDFGENVVLEGNISL
                     QEKKLWLEFINKSDSEVKFNGNDAELYFEEDNFDTFVERLSTMKGIDYVHLAIEHRWG
                     QRAIRFYDLDGHIIEIGETMSSVCRRFLDSGLSIDEVAKRMDVTVEYIESVLE"
BASE COUNT          361 a          161 c           60 g          356 t
ORIGIN      
        1 tataagctaa tacttatatt tttctaaatt taaatcatcc aataaatatt aaattctatt
       61 ataatatgta aggaaaatca tgcacttttt aaaaatccaa ataaaattat aacaaatatt
      121 atattctgta tcttacaaaa acaaaaactg taagtaaatt tgtttccata caagtttact
      181 tacagccctt acttctatac aaaatatagt tttactttaa tataatccat ctattctaaa
      241 actgattcaa tatattcgac agttacatcc attcttttag ctacctcatc tattgaaaga
      301 ccactatcta aaaaacgtct acacacagat gacatagttt ctccaatctc aataatatgc
      361 ccatctaaat cataaaatct aattgctctt tgaccccacc tatgctctat agctagatga
      421 acataatcta tacctttcat tgtactcaat ctttcaacaa aagtatcaaa attatcttcc
      481 tcaaaatata actcagcatc atttccattg aacttaactt cactgtcact cttattaata
      541 aattctaacc aaagcttttt ttcttgtaat gatatattac cttctaatac aacattttct
      601 ccaaaatcac agattacctt taaccccaaa atatcataat aaaacttttt tgatttctca
      661 atatcttcaa ctactattaa aacatttaaa aacttcatat tacctcctta atatacacat
      721 actttattta taatatataa ttagaattaa actataaagg tttcaataag gtctagtata
      781 aagaaaaaaa ttataagtct tattgaaata gttaagttaa ttttatcata taatataaat
      841 tttaatcttg taaaaaacag acatgttcat acttttaggt ttaaatctat atgtattctt
      901 attaattttg ctaaaactgt tatttttgta gactattt
//
LOCUS       DAMJWP010000414          932 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.421, whole genome shotgun sequence.
ACCESSION   DAMJWP010000414 DAMJWP010000000
VERSION     DAMJWP010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.421"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(122..805)
                     /locus_tag="PC700_003701"
     CDS             complement(122..805)
                     /locus_tag="PC700_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HDF2582914.1"
                     /translation="MTSKSQMSESFFLCSLLAITGGFLDIYTYVVRGKVFANAQTGNI
                     VLFGLNIAEGNVKESFYYLIPILAFMLGVFIAEKIRKYFTNNKTKIHWRQVIIIIEMI
                     TILIVSFIPRGRFNMFVNVAISFICSMQVESFRKVNGNSFATTMCTGNLRSATETLFH
                     YIHTKNIFMIKKSLQYYAIIIFFISGAILGTFFTKIFVEKSILICFFILAVVFGIMFI
                     NKDDSFNEN"
BASE COUNT          367 a          146 c           74 g          345 t
ORIGIN      
        1 tacttctatt aacttatata ttgcttacta tattttaaat tctttaatta atctatacaa
       61 tatttgctat atataaagta tatcaaataa cagcttcatt tttttgtaaa tttacagaaa
      121 gttaattttc attaaatgaa tcatctttat tgataaacat aattccaaag accactgcca
      181 aaataaaaaa acatattagt attgattttt ccacgaaaat ttttgtaaaa aaagttccta
      241 aaatagctcc agatataaag aatataataa tagcataata ctgtaaactc ttttttatca
      301 taaaaatatt cttagtatgt atataatgaa ataaagtttc tgtagcactt cttaaatttc
      361 cagtacacat tgtcgttgca aaactattcc catttacttt tctaaaactc tctacttgca
      421 ttgaacaaat aaaagaaata gcaacattta caaacatatt aaatcttcct ctaggaataa
      481 atgaaacaat aagtattgta atcatttcaa ttataatgat aacctgacgc caatgaatct
      541 ttgtcttatt atttgtaaaa tactttctta tcttctcggc tataaataca cctaacataa
      601 atgccaaaat aggaatcaaa taataaaatg attcctttac attaccttct gctatattaa
      661 gcccaaataa aactatattt ccagtttgtg cattagcaaa gactttacct cttaccacat
      721 aagtataaat atccaaaaat cctcctgtta ttgccaacaa agaacataaa aagaatgatt
      781 cagacatctg actcttgctt gtcatatata aacctctcgc tttctaatga tagatacaaa
      841 atcaaaatca ttttagattc tatttttcat tgatataaaa aaattattta tatttaaata
      901 ttatctatat ttatacatca tttatattta ta
//
LOCUS       DAMJWP010000415          924 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.422, whole genome shotgun sequence.
ACCESSION   DAMJWP010000415 DAMJWP010000000
VERSION     DAMJWP010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..924
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.422"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            8..868
                     /locus_tag="PC700_003702"
     CDS             8..868
                     /locus_tag="PC700_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2582915.1"
                     /translation="MLLKKDRITFSEILILIVGCILMAISLNLFFNPHAIAAGGITGL
                     GVVLNSLFGVELWIVNLLLNVPLFIFAYKILSKKDCFKTVLGIIFLTIALKLTANMAT
                     LDITNDMYLAIISGSILMGVGQGLIFRINGSTGGTDLMALLLNKYFPTFSIPVLMGIV
                     DCVVVVLSGIVNRQVEIALYSTVALYILVKVSDLLIEGFNYSKSFTIISDLSKDISKK
                     IMEDLDRGATILKGEGAYTGENKNVLLVVVEKKEVVELKKLVKNVDPNAFIIITDIHE
                     ALGNGFKKIE"
BASE COUNT          333 a          103 c          148 g          340 t
ORIGIN      
        1 aggagtgatg ttattgaaaa aagatagaat aactttttca gaaattttaa tattaattgt
       61 aggctgtata ttaatggcca tttctcttaa tttatttttt aatccacatg caatcgctgc
      121 tggtggtata acaggacttg gagttgtttt aaattcatta ttcggtgtag aactttggat
      181 tgtcaattta cttttaaatg ttccattatt catatttgct tacaaaatac tttcaaaaaa
      241 agattgtttt aaaacggttt taggaataat ttttcttacc atagcactta agcttactgc
      301 aaatatggct acattagata taacaaatga tatgtatctt gcaattatat ctggttctat
      361 tttaatggga gttggtcaag gacttatttt tagaataaat ggctctacag gtggaactga
      421 tttaatggca ctcctactaa ataaatattt tcctacattt agtatacctg ttttaatggg
      481 tattgtagac tgcgttgtag ttgttttatc aggtatagta aatagacagg ttgaaattgc
      541 actttattca actgtggctt tatacatttt agtaaaagtt agtgacttgc ttattgaagg
      601 atttaattat tccaaatcat tcactattat atctgacctt tcaaaagata taagtaaaaa
      661 aataatggaa gatttagata gaggtgcaac tattctaaaa ggagaaggtg catatactgg
      721 agaaaataaa aatgtacttt tagtagtagt tgaaaagaaa gaagttgtcg aattaaaaaa
      781 attagtaaaa aatgtcgacc ctaatgcttt tattataatt acagatatac atgaagcttt
      841 aggtaatgga tttaaaaaaa tagaataact aacaataaat agcaagtata attaacatat
      901 acaaattcaa ctttttatat gtta
//
LOCUS       DAMJWP010000416          915 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.423, whole genome shotgun sequence.
ACCESSION   DAMJWP010000416 DAMJWP010000000
VERSION     DAMJWP010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..915
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.423"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            65..820
                     /locus_tag="PC700_003703"
     CDS             65..820
                     /locus_tag="PC700_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1835 domain-containing protein"
                     /protein_id="HDF2582916.1"
                     /translation="MAHICFEDICYLLLKEAMKLKLIPHNKIVLFEDDLSIGNIFDVN
                     NYELRENIFKIRNVFENNPKLLFKPDLLEDRKLSKCRNIIIWCANNPKDLCGVYYVVS
                     KFQDKNIKIALVDEKKTEDALIRYSFTSEMSPEDFPFFLDKTLEIGFNEKLEFKNKWD
                     VLVKENSIFRALVGNEIKSVEETYFDKYILRNISDKWQPLIEVVANCMFEDEMRVKDW
                     FILWRLEKMASLKIVKIKGSKGDFYSDKEIRKV"
BASE COUNT          389 a           59 c          155 g          312 t
ORIGIN      
        1 tttgataatt acagtttata tattataata aaataaattg ttaattttaa aagaggtgaa
       61 ttttatggca catatatgct ttgaggatat ttgttatttg ttattaaaag aagcaatgaa
      121 gttaaagtta ataccacata ataaaattgt attatttgaa gatgatttat caataggtaa
      181 tatttttgat gtaaataact acgaattaag agaaaatatt tttaagatta gaaatgtttt
      241 tgaaaataat ccgaaactat tattcaagcc agatttgtta gaagatagga aattatcaaa
      301 atgtagaaat ataataatat ggtgtgcaaa taacccaaaa gatttgtgtg gagtatatta
      361 tgtagtgagt aagtttcaag ataaaaatat aaagattgca ttagtagatg aaaaaaagac
      421 agaagatgct ctaattagat attcatttac cagtgaaatg tctccagaag attttccatt
      481 ctttttagat aagacactag aaattggctt taatgaaaaa ctagaattta aaaataaatg
      541 ggatgtgtta gttaaagaaa attctatttt tagagcatta gtaggtaacg aaattaaaag
      601 tgttgaagaa acttattttg ataaatatat attaagaaat atcagtgaca aatggcaacc
      661 tttaattgaa gtggttgcaa actgtatgtt tgaagatgaa atgcgtgtaa aagattggtt
      721 tatactttgg agattagaga agatggcaag tttaaaaata gtaaaaataa aaggaagtaa
      781 aggggatttt tattctgata aggagataag aaaagtttaa aaattgaatt tataataaat
      841 tgtattaaat aatgggtata actatgaaga aaaatatctt aacagtatta atattataat
      901 tagcaataag tataa
//
LOCUS       DAMJWP010000417          899 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.424, whole genome shotgun sequence.
ACCESSION   DAMJWP010000417 DAMJWP010000000
VERSION     DAMJWP010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.424"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            117..674
                     /locus_tag="PC700_003704"
     CDS             117..674
                     /locus_tag="PC700_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2582917.1"
                     /translation="MRVSREIVIQATSDIVNKDGLNKVSLKVIAEKLGIRTPSLYNHI
                     ESLDDLLLEVAHKGMREMNSQMIKATIGNFGDTAIKCISIAYFNFIISNPGVYETIQW
                     ATWHGNNETLEIFDEYKSLLEKLILSCNLNTKNTGEVLNLIMSVLHGYSTLQLGKSLL
                     SKEEAIKGLADSIDTVLLGIHKKYD"
BASE COUNT          368 a           89 c          144 g          298 t
ORIGIN      
        1 aaactgtcaa gattttataa gagtataaag aattttataa attacaataa ttgacgaaac
       61 taattagatt agctaaacta ataaagtaat taattttaaa tgaaagaggg aaaatcatgc
      121 gtgttagtag agaaatagtt atacaagcta catcagatat agttaataaa gatgggctta
      181 acaaagtttc tttaaaagtt attgcagaaa agctaggaat acgtacacca tctttgtaca
      241 atcatataga aagtctagat gatttattac ttgaagtagc tcataaagga atgagagaga
      301 tgaatagtca aatgataaag gcgactattg gaaattttgg agatacagct ataaagtgta
      361 taagtatagc atactttaat tttataattt ctaatcctgg tgtttatgaa acaattcagt
      421 gggctacttg gcatggaaat aatgaaacat tagaaatctt tgatgaatat aaatccttat
      481 tagaaaaact tattctttct tgtaatctaa atacaaagaa tacaggtgaa gtattaaact
      541 taataatgag tgtacttcat ggttattcga ctttgcaact tgggaaatca cttttgagta
      601 aagaagaagc tataaaaggt ttagccgatt ctattgatac tgtgttatta ggtattcata
      661 aaaaatatga ttaaggaggt aaataaatta ttttaactta tttattaaac acttcattac
      721 ggagcatgta aattaaaatt ttatcatttg taccaaagga aaatcaacag gagaacagta
      781 tattttaaga atatttattt tcaaatgccc aatattgttt acaaagaaga gatataaaga
      841 gaaacaacat agtttatgtt taataattga attaatttat atgttaatca aaaaggata
//
LOCUS       DAMJWP010000418          890 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.425, whole genome shotgun sequence.
ACCESSION   DAMJWP010000418 DAMJWP010000000
VERSION     DAMJWP010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.425"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..621
                     /gene="cdtR"
                     /locus_tag="PC700_003705"
     CDS             <1..621
                     /gene="cdtR"
                     /locus_tag="PC700_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family response regulator CdtR"
                     /protein_id="HDF2582918.1"
                     /translation="LLKELTEKNKVKIYFIVVDAKYKISNELCDGLWIAQKIRESDYI
                     SPIIFLTNHIEMILGIFDYRLEVMDFILKHDMEIAESKIKACIKIAHKRYVKEKNYRS
                     NFFTIYSDSSLWKISFDEVIYFETSAIPHKIKLVTTSRIFEFYKSLRSLSDLDACFIR
                     VHKSFVVNKYHIVSLDLKKNNIKMSNGHICRISNTYRNILKNIIKT"
BASE COUNT          368 a          101 c          109 g          312 t
ORIGIN      
        1 ttattaaaag aacttactga aaaaaataaa gtaaaaatat attttatagt tgtagatgca
       61 aaatacaaga taagtaatga attatgtgat ggattatgga tagcacaaaa aattagagaa
      121 tcagattata ttagtccaat catatttctt acaaatcata tagaaatgat tttagggatc
      181 ttcgattata ggttagaagt tatggacttt atattaaaac atgatatgga aattgctgaa
      241 agtaaaataa aagcttgtat taaaattgct cataaaagat acgttaaaga aaaaaattat
      301 cgctctaatt tttttactat ctactcagat tcctcactat ggaaaatttc atttgatgaa
      361 gttatatatt ttgaaacaag cgctattcca cataaaataa aattagtgac tacttcaaga
      421 atatttgaat tctataaaag tttaaggtct ttatcagatt tagatgcatg ctttattcgt
      481 gtacataaat cttttgtagt taataaatat catattgttt ctctcgacct aaagaaaaat
      541 aatattaaaa tgagtaatgg ccatatatgt cgtatatcta atacatatag aaatatttta
      601 aagaacatta ttaaaacata ataaatagaa aaaagagatg tctcaagata agaatttgaa
      661 ccatctcttt ttttatacaa aaaaagtagt tcctaagaat cctctatata ataatcgttt
      721 tgtgaaaaaa cacataatga ctatatcata tacaatttct attagacatt gcaaccattg
      781 ttcctagatt atgattcaac aagatagctc attaaatcat aaaagtatga aatagtctct
      841 ttaaaatatt cagttgttat tttgtactga catatcatat aaatacatat
//
LOCUS       DAMJWP010000419          835 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.426, whole genome shotgun sequence.
ACCESSION   DAMJWP010000419 DAMJWP010000000
VERSION     DAMJWP010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..835
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.426"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(412..588)
                     /locus_tag="PC700_003706"
     CDS             complement(412..588)
                     /locus_tag="PC700_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582919.1"
                     /translation="MFIGEIDKCTHILTAYISSSYDYCNFLDTQLDDFISEYGETVVE
                     TCLHQVLLLVSRYN"
BASE COUNT          306 a           88 c          113 g          328 t
ORIGIN      
        1 aaatttgtct agttatacgt ttttattgac cagttatgca gttgatattg aatattctaa
       61 atattggtgt tattttatag gtggtttaaa catattaggg ggattttata tatgatttgt
      121 atgatttata gagaatgtat aaaatagtaa aggtttagag catgtttagt ggattgcaga
      181 atatgcatat agaatgattc ttgtaaatta agttagacat aggaaatata taatttgtat
      241 tggatgattg attttatggt gtatgctttt aacttctata atgggaaagt aaagatgtac
      301 tcccaataca tctttacttt agcagaagtt atagttactt ataaagtaca aaaaattata
      361 tatttattag agtagataaa tctaaaaagg tttttctaga aaaatttaat tttaattgta
      421 tctacttact aataataaga cttgatgtaa gcaggtttca actaccgttt ctccatattc
      481 tgatataaaa tcatctaact gtgtatctaa aaaattacaa taatcatatg aactactgat
      541 gtaagcagtt agtatatgtg tacatttgtc tatttctcca ataaacatat tatccatccc
      601 cctaaaaaat ttagtaaaaa taaaaagtcg cattctatta gtcaattaac ataatttata
      661 aaatttttta gtactagttt ctataactta actccttaag gttaaatatt cccagtttta
      721 ccaatttagt tttattctac aagtatataa tactagaaga tgcttcatta ttcaacatgc
      781 ttgtttgaga atagaaaata ttctaaaatt ataattgttt ttaaaaataa gattc
//
LOCUS       DAMJWP010000420          817 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.427, whole genome shotgun sequence.
ACCESSION   DAMJWP010000420 DAMJWP010000000
VERSION     DAMJWP010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..817
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.427"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            73..771
                     /locus_tag="PC700_003707"
     CDS             73..771
                     /locus_tag="PC700_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2582920.1"
                     /translation="MEKKYFEIHGVPSILWGNPSSKLYIYIHGQCGNKEEAEAFANIA
                     VPHEYQVLSVDLPEHGERKREKNSFNPWHVVPELVSIMEYAKCHWNQISLYANSIGAW
                     FSMLSFEKENLRECIFVSPILDMQQLISNMMLWANVSEEQLKHELIIPTSFGHTLSWE
                     YLQYVKQHPITQWNVPTKILYGKHDELTEIGVVEKFVEKFNCNLTILENGKHWFNTPQ
                     ELNSLYEWLNTLVK"
BASE COUNT          309 a          109 c          129 g          270 t
ORIGIN      
        1 tatattacaa cattatttta tagtttgatt aattcaacaa tcactaatat atcaaactga
       61 aaggagattt ttatggaaaa aaaatatttc gaaattcatg gagttccttc aatcctttgg
      121 ggaaatcctt caagtaaatt atatatttac atacatggac aatgcggcaa taaagaggag
      181 gctgaagctt ttgctaatat tgcagttcca catgagtatc aagttttaag tgttgatttg
      241 cctgaacatg gagaacgtaa aagagaaaaa aattcattta acccatggca tgttgttcca
      301 gaattagtat ctattatgga atatgcaaag tgccactgga atcaaatttc gttatatgca
      361 aatagcattg gagcatggtt tagtatgtta agctttgaaa aagagaactt aagagagtgt
      421 atttttgtat ctccaattct agatatgcaa cagctcattt caaatatgat gctttgggca
      481 aatgtatcag aagaacagct taaacatgaa ctaattatcc caacttcatt tgggcatact
      541 ctttcatggg aatatctaca atatgtaaaa caacatccta tcactcaatg gaatgtacca
      601 acaaaaatat tatatggtaa acatgatgaa cttactgaaa tcggtgttgt agagaaattt
      661 gtagaaaagt ttaattgcaa ccttactatt ctggaaaatg gcaaacattg gtttaacaca
      721 ccacaagaat taaattcatt atatgaatgg ttaaatactt tagttaaata aaaaataatt
      781 atattattaa tatgattaaa ttatttataa atgttat
//
LOCUS       DAMJWP010000421          814 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.428, whole genome shotgun sequence.
ACCESSION   DAMJWP010000421 DAMJWP010000000
VERSION     DAMJWP010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.428"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            111..770
                     /gene="cprR"
                     /locus_tag="PC700_003708"
     CDS             111..770
                     /gene="cprR"
                     /locus_tag="PC700_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CprR"
                     /protein_id="HDF2582921.1"
                     /translation="MSKILVVDDELDMLKLIENVLKRDGHEVKVISDVEKAMNMDFNY
                     FNLIILDVMMPKMDGFEVCKTIRNKVDCPILFLTAKNMESDIMYGLGIGADDYITKPF
                     GVGELRARITAHLRRESREKKNLLTISNVQFNFLGKEVSIDGKKINFTKSEYLICEFL
                     AKNKGQVFSKERIYESVYGFDGESDISTITEHIKNIRSKLKQYDIYTIETVWGIGYKW
                     V"
BASE COUNT          330 a           56 c          163 g          265 t
ORIGIN      
        1 ttaattattg taagctataa tatgagagta ttatggttaa tgtgaatttt aaagatatta
       61 ttaaagatat aaattagaat actattaaac ggataaatgg aggagttatt atgtctaaga
      121 tattagtagt agatgatgag ttagatatgt taaagttgat tgaaaatgtg ttgaaaagag
      181 atggacatga agtaaaggta attagtgatg tagaaaaggc tatgaacatg gattttaatt
      241 attttaatct aataatatta gatgttatga tgcctaagat ggatggtttt gaagtatgta
      301 aaactataag aaataaggtt gattgtccaa tactgttttt gacagcaaaa aatatggaaa
      361 gtgatattat gtacggtctt ggcataggtg cagatgacta tataaccaaa ccttttggtg
      421 taggagaatt aagggctagg ataactgctc atttaaggcg agaaagtaga gaaaaaaaga
      481 atttattaac tatctcaaat gttcaattta actttttagg caaagaagtt tcaattgatg
      541 gtaaaaaaat taattttact aaaagtgaat atctaatatg tgaatttcta gcaaaaaata
      601 agggacaagt attttcaaaa gaaagaatat atgagtcggt atatggtttt gatggagaaa
      661 gtgatataag tactatcaca gagcatataa aaaatatacg aagcaaatta aaacaatatg
      721 atatttatac aatagaaact gtatggggga tagggtataa atgggtgtaa taagtataag
      781 tgttgcttag tgaaaatatt atatatatct acaa
//
LOCUS       DAMJWP010000422          811 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.429, whole genome shotgun sequence.
ACCESSION   DAMJWP010000422 DAMJWP010000000
VERSION     DAMJWP010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.429"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            86..733
                     /locus_tag="PC700_003709"
     CDS             86..733
                     /locus_tag="PC700_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2582922.1"
                     /translation="MNILIADDEISMLKILCAYFKKENFNVFTAKNGEEALDIFYENK
                     IDLAILDWMMPIIDGIEVCKEIKENSNTRVLMLTAKSQSEDEIEALNIGADEYIKKPF
                     DPRVLIIRAKKLINYKNTINIKDLKIDFGSSKVFKGDKELLLTKKELELIRCLINNKG
                     MVLSREKLLDLVWGLDYEGDFRTVDTHIRRLRVKIGEDIIKTYRGLGYSLEDFND"
BASE COUNT          345 a           55 c          148 g          263 t
ORIGIN      
        1 taaacttcct gtaacatata ttttatataa ttaaagtaat ggatataata tgaaatataa
       61 taatgaattt tagggatgtg aataaatgaa catattaatt gctgatgatg aaattagtat
      121 gcttaagata ctttgtgcat attttaaaaa agaaaacttc aatgtgttta ctgcaaaaaa
      181 tggggaagag gctttagata ttttttatga aaataagata gacttagcta tattagactg
      241 gatgatgcca ataattgatg gaatagaggt ttgtaaagag ataaaggaaa atagtaatac
      301 aagggtactt atgttgactg ccaaaagtca aagtgaagat gaaatagaag cactcaacat
      361 tggagcagat gaatatataa aaaaaccatt tgatcctaga gttttaataa taagagctaa
      421 gaaactaata aattataaaa atacaattaa tattaaagat ttaaaaatag attttgggtc
      481 aagtaaagtt tttaaaggtg ataaggaatt attattgact aagaaagaat tagaattaat
      541 tcgttgttta atcaataata aaggaatggt tttatctagg gaaaaacttt tagatttagt
      601 atggggattg gattatgagg gagattttag aacagtagat actcatataa gaagattaag
      661 ggtaaaaata ggagaagata taataaaaac ttatagagga ttagggtata gtctggagga
      721 ttttaatgac taaactaaac aaaaaattgt ccataagtat atctatagta gttgtagttg
      781 tttatataat atctataatt ataaatagta t
//
LOCUS       DAMJWP010000423          807 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.430, whole genome shotgun sequence.
ACCESSION   DAMJWP010000423 DAMJWP010000000
VERSION     DAMJWP010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.430"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            131..385
                     /locus_tag="PC700_003710"
     CDS             131..385
                     /locus_tag="PC700_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582923.1"
                     /translation="MENKDFKLTICIFAFVIGVVVLFCCIGLGEFSAEHIIQQNGGIM
                     DTDQYNIYLDQSISQYRNFGSILALLGGIGILFDKYIRKY"
     gene            430..753
                     /locus_tag="PC700_003711"
     CDS             430..753
                     /locus_tag="PC700_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582924.1"
                     /translation="MATEIFTVKITPLEAQKMIKENIDADLVFSDSYNLGDDKFILIT
                     TFEKYYARNNSDAGLIVVYENTTEKTVIKATSTGSAIGVLKIDWGAGGNLLKRVKNIL
                     AEYII"
BASE COUNT          329 a           70 c          134 g          274 t
ORIGIN      
        1 tttaaatata taaaactata aaggttacaa ttttggaaat agatgtaaat atatgttcta
       61 ttgtataata agagtaatat aaaatctagt aaaaataatc aaataaattt aacttatgag
      121 agggataact atggaaaata aggattttaa attaacaata tgtatatttg cttttgtaat
      181 tggagtagta gtattattct gctgtatagg tttgggtgaa tttagtgcag agcatattat
      241 tcaacaaaat ggtggaatca tggatactga ccaatataat atttatctag accaatctat
      301 ttctcaatat agaaattttg gttctatatt agctctgtta ggaggaatag ggatattgtt
      361 tgataaatat atcagaaaat actagagatt atgttttgtc taaaataaat tcttatgagg
      421 agataaaaaa tggcaacaga aatatttact gtaaaaatta cacccttaga agcacaaaaa
      481 atgataaaag aaaatattga tgcagattta gtttttagtg attcttataa tttaggagat
      541 gataaattta tactaattac aacttttgag aagtattacg caagaaataa tagtgatgca
      601 ggattaatcg tagtttatga aaataccaca gaaaaaacag ttattaaagc tacttcaaca
      661 ggcagtgcaa ttggagtttt aaaaattgat tggggtgcag gtggaaattt acttaaaaga
      721 gtaaaaaaca tacttgctga atatattatt taattatttt atctaactga taatatgtca
      781 agaaaatctc gtaaaagagg ttaagaa
//
LOCUS       DAMJWP010000424          789 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.431, whole genome shotgun sequence.
ACCESSION   DAMJWP010000424 DAMJWP010000000
VERSION     DAMJWP010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.431"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            34..>789
                     /locus_tag="PC700_003712"
     CDS             34..>789
                     /locus_tag="PC700_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDF2582925.1"
                     /translation="MFAEERIQAILNILKKEGRVTVKELSSKFNVTEDCIRKDLKNIE
                     KISSIRRIYGGAVLARETLENQDTKDRKEINIPTKKIIAEKAFNLIEDRETIFLDIST
                     INILLAKLLAESNKKVTLVTNMLDILNVVTSKPNNLNIISTGGLLNLSLDGFVGTPTI
                     EFISKYKFDKTFMGSCGVDVFNSSLTTFEIEDGLTKKAIINSSKKVFIVMEDKKFKFD
                     GNFKFAHLEDISSIITEKAPTPDIVDILSEFNVN"
BASE COUNT          329 a           84 c          130 g          246 t
ORIGIN      
        1 actataatac tatattgcga aaagaggtag aaaatgtttg cagaagaaag aatccaagct
       61 atattaaata tcctaaaaaa agaaggtaga gttactgtca aagagctcag ttctaaattt
      121 aatgttacag aagattgtat aagaaaagat ttaaaaaata tagaaaagat ttcttcaata
      181 agaagaattt atgggggagc tgtactagct agagaaacac tagagaatca ggatacaaaa
      241 gacagaaaag aaattaatat tccaacaaag aaaataatag cagaaaaagc atttaattta
      301 attgaagata gagaaactat atttttagat atctctacaa ttaacatctt gcttgcaaag
      361 ttgttagctg aaagtaataa aaaagtaaca cttgttacaa atatgttgga tatactaaat
      421 gtagttactt caaaaccaaa taatttaaat ataatttcca ctggaggttt attaaatctc
      481 agtttagacg gttttgtcgg tactccaact atagagttta tatcgaaata taagtttgat
      541 aagactttta tgggaagttg tggagtagat gtattcaact cttcacttac aacttttgaa
      601 attgaagatg gtctcacgaa gaaggctata ataaattcaa gtaaaaaagt atttatagtt
      661 atggaagata aaaagtttaa atttgatgga aatttcaagt ttgctcattt agaagatatt
      721 agttctataa ttacagaaaa agcgcctact cctgatatag tagatatttt atcagaattt
      781 aatgtaaat
//
LOCUS       DAMJWP010000425          768 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.432, whole genome shotgun sequence.
ACCESSION   DAMJWP010000425 DAMJWP010000000
VERSION     DAMJWP010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.432"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            57..722
                     /locus_tag="PC700_003713"
     CDS             57..722
                     /locus_tag="PC700_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582926.1"
                     /translation="MKGNIVQYNFADIEEEVYSLDYAIAWNTDEENVNIIPFTNKFCK
                     ESIESFCLGKINNFVEILNEGFVENHHYVHLDKMISVPKKKVNLVYQQDTHGYLLRDD
                     NDNLIPAKITSEQSKSISSKMELFCAGEEKCLINILLKADPSYILDVDSIKDKNILNL
                     GYESIDRYKEYNFDDDKILIFFINKKRYSVIMKKTNNSDNDLVSRNNAIKELFTNKAG
                     NLN"
BASE COUNT          325 a           73 c          109 g          261 t
ORIGIN      
        1 taatataagt tttataaagt atattaaata tattataaga tggaggtcga gtaattttga
       61 aaggaaacat agtacaatat aattttgcag atatagagga agaagtatat tcgttggatt
      121 atgctatagc atggaataca gatgaggaaa acgtaaacat aataccattt acaaataaat
      181 tctgtaaaga atctatagaa tctttttgcc taggtaaaat taataacttt gttgaaatat
      241 taaatgaagg ttttgttgaa aatcatcatt atgtacattt agataaaatg attagtgttc
      301 ctaagaaaaa ggttaattta gtatatcaac aagacacaca tggttatctt ctaagagatg
      361 ataatgacaa ccttattcct gcaaaaataa cttcagagca atctaaatct atatcaagta
      421 agatggagtt attctgtgct ggtgaagaaa aatgtttaat taatatatta ctaaaggctg
      481 acccatcata tattttagat gtagatagta taaaagacaa aaacattcta aatctaggtt
      541 atgagtctat tgacagatat aaagaataca attttgatga tgataagatt ttaatatttt
      601 ttataaataa aaaacgttat tcagtgatta tgaaaaaaac aaacaactct gataacgatt
      661 tagtttccag aaacaatgct attaaagaat tatttactaa taaagcagga aatcttaact
      721 aatattataa ttttaaatta tattattaat taaattaata atagtata
//
LOCUS       DAMJWP010000426          767 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.433, whole genome shotgun sequence.
ACCESSION   DAMJWP010000426 DAMJWP010000000
VERSION     DAMJWP010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.433"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            38..580
                     /locus_tag="PC700_003714"
     CDS             38..580
                     /locus_tag="PC700_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2582927.1"
                     /translation="MKICITVGHSILKGGKSTGVNGIVDEYNYNKKLAPMLAEMLISQ
                     GNTVDVIICPERHFMSEREEFFYRVPKVNSGKYDLLVELHLNKADGTQCGTEVLYYGT
                     EGLEYAKRVSNRLGELFENRGAKKRENLYILRNTNPVAIQIESFFCDNVNDCNKANES
                     GYDYIARLITEGILNKDINM"
BASE COUNT          321 a           62 c          139 g          245 t
ORIGIN      
        1 atataaaaat tggcttaaaa gaaggtgaaa aaagtaaatg aaaatatgca taacagtagg
       61 tcatagtata ctaaaaggtg gtaaaagtac aggtgtaaat ggtatagttg atgaatacaa
      121 ttataataaa aaattagctc ctatgttagc tgagatgctt ataagtcaag gtaatacagt
      181 agatgtaata atttgtccag aaaggcactt tatgtcagaa agagaagaat ttttctatag
      241 agttccaaaa gtaaacagtg gtaaatacga tttattagta gagctacatt taaataaagc
      301 tgatggaaca caatgtggta cagaagtgct ttactatggc actgaaggat tagaatatgc
      361 taaaagagta tctaatagac ttggagaatt atttgaaaat agaggagcta aaaaaagaga
      421 aaatttatat atattgagaa atacaaatcc agttgcaata cagattgaaa gttttttctg
      481 tgataatgtg aatgattgta acaaagcaaa tgaatcaggt tatgattata tagcacgatt
      541 gattacagaa ggtatattga ataaagatat aaatatgtga gattatggtg atttaggtat
      601 tgattaatac cttttaatat tagatataaa taaaaataca atgtattagt ataatttaaa
      661 ctttcaaaat atagattagt ttgattattg acaagagata cctcctaaat attgatttat
      721 cagtatattt aaggaggtat ttaaatataa aaatttaatt gatttgc
//
LOCUS       DAMJWP010000427          760 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.434, whole genome shotgun sequence.
ACCESSION   DAMJWP010000427 DAMJWP010000000
VERSION     DAMJWP010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.434"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..>760
                     /locus_tag="PC700_003715"
     rRNA            <1..>760
                     /locus_tag="PC700_003715"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          204 a          168 c          239 g          149 t
ORIGIN      
        1 caggatggac ccgcgtctga ttagctagtt ggtaaggtaa cggcttacca aggcgacgat
       61 cagtagccga cctgagaggg tgatcggcca cattggaact gagacacggt ccaaactcct
      121 acgggaggca gcagtgggga atattgcaca atgggcgaaa gcctgatgca gcaacgccgc
      181 gtgagtgatg aaggccttcg ggtcgtaaaa ctctgtcctc aaggaagata atgacggtac
      241 ttgaggagga agccccggct aactacgtgc cagcagccgc ggtaatacgt agggggctag
      301 cgttatccgg atttactggg cgtaaagggt gcgtaggcgg tctttcaagt caggagtgaa
      361 aggctacggc tcaaccgtag taagctcttg aaactgggag acttgagtgc aggagaggag
      421 agtggaattc ctagtgtagc ggtgaaatgc gtagatatta ggaggaacac cagttgcgaa
      481 ggcggctctc tggactgtaa ctgacgctga ggcacgaaag cgtggggagc aaacaggatt
      541 agataccctg gtagtccacg ctgtaaacga tgagtactag gtgtcggggg ttaccccctt
      601 cggtgccgca gctaacgcat taagtactcc gcctgggaag tacgctcgca agagtgaaac
      661 tcaaaggaat tgacggggac ccgcacaagt agcggagcat gtggtttaat tcgaagcaac
      721 gcgaagaacc ttacctaagc ttgacatccc aatgacatct
//
LOCUS       DAMJWP010000428          752 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.435, whole genome shotgun sequence.
ACCESSION   DAMJWP010000428 DAMJWP010000000
VERSION     DAMJWP010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.435"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..>752
                     /gene="tuf"
                     /locus_tag="PC700_003716"
     CDS             <1..>752
                     /gene="tuf"
                     /locus_tag="PC700_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDF2582928.1"
                     /translation="ELLELVEMEVRDLLTEYDFPGDDTPIVRGSALMALEDPKSEWGD
                     KIVELFEQIDEYIPAPERDTDKPFLMPVEDVFSITGRGTVATGRVERGVLKVQDEVEL
                     VGLTEAPRKVVVTGVEMFRKLLDQAQAGDNIGALLRGVQRNEIERGQVLAKTGSVKAH
                     TKFTAEVYVLKKEEGGRHTPFFDGYRPQFYFRTTDVTGACKLPEGIEMVMPGDNVTME
                     VDLINSIVVEEGLRFSIREGGRTVASGVVATI"
BASE COUNT          310 a           94 c          187 g          161 t
ORIGIN      
        1 gagttattag agttagtaga gatggaagta agagatttat taacagaata tgatttccca
       61 ggagatgaca ctccaatagt aagaggttca gcattaatgg cattagaaga tccaaagagc
      121 gagtggggag ataagatagt agaattattc gagcaaatag atgagtatat accagctcca
      181 gagagagata cagataagcc attcttaatg ccagtagagg atgtattctc aatcacagga
      241 agaggaacag ttgcaacagg aagagtggaa agaggagtac taaaagtaca agacgaagta
      301 gaattagtag gattaacaga agcaccaaga aaagtagtag taacaggagt agagatgttc
      361 agaaaattat tagaccaagc acaagcaggg gataatatag gagcattatt aagaggagta
      421 caaagaaacg agatagaaag aggacaagta ctagcaaaga ctggatcagt aaaggcacac
      481 acaaagttta cagcagaagt atatgtactt aaaaaagaag agggtggaag acatacacca
      541 ttctttgatg gatatagacc acaattctat ttcagaacaa cagacgtaac aggagcttgt
      601 aagttaccag aaggaataga gatggtaatg cctggagata acgtaacaat ggaagtagac
      661 ttaataaact caatagttgt agaagaggga ttaagattct caataagaga aggtggaaga
      721 acagtagctt caggagttgt tgctacaata at
//
LOCUS       DAMJWP010000429          743 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.436, whole genome shotgun sequence.
ACCESSION   DAMJWP010000429 DAMJWP010000000
VERSION     DAMJWP010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.436"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..>743
                     /locus_tag="PC700_003717"
     CDS             <1..>743
                     /locus_tag="PC700_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HDF2582929.1"
                     /translation="KIYEENEKWKEYNIQNTMSKYIGIPESEVIFISNLSQYVSFLSE
                     LDNAGDYVSRGQKDCMYELLPSLHRKYKKDYGIHSDEYEEAFKQKIIYYDKEIKNRSS
                     EELRAEGQHFGLPTEYLDFTEAHLISLLFAIEDYKYTKQHSIVYFIDSISYNTDTIKR
                     SEKLVNYSDTSAIDNCSKFSSRSFFIKVGNSNERIHFQKGCFLKVSSEDKGEFKEKLK
                     KYCKVVVINKDAKKEILKELFNLGITFEN"
BASE COUNT          329 a           72 c          111 g          231 t
ORIGIN      
        1 aaaatatatg aagaaaatga aaagtggaaa gagtataata tacaaaatac tatgtctaaa
       61 tatataggta tacctgaatc agaagttata tttatatcaa atttgagtca gtatgtttca
      121 tttttatctg aactagataa cgcaggcgac tatgtatcta gagggcaaaa agattgtatg
      181 tatgaattat taccatcatt acatagaaaa tataaaaaag actatggtat acattcagat
      241 gaatacgagg aagcattcaa acaaaaaata atttattatg ataaagaaat aaaaaataga
      301 tcgagtgaag aattaagagc tgaagggcaa cattttggac taccaacaga atatttagat
      361 ttcacagaag ctcatttaat atcattatta tttgcaatag aagactataa atatacaaaa
      421 cagcattcaa ttgtgtactt tatagactct atttcttata atactgacac tataaaaaga
      481 tctgagaagt tagtaaatta ttcagataca tctgcaatag ataattgtag taaattttca
      541 tcaagatcat tttttataaa ggtagggaat tcaaatgaaa gaatacattt tcaaaaagga
      601 tgtttcctaa aagtatcatc agaagataaa ggcgaattta aggaaaaatt aaaaaagtat
      661 tgtaaagtag tagttattaa taaagatgct aaaaaagaaa tattaaaaga attatttaat
      721 ttaggtatta catttgaaaa tat
//
LOCUS       DAMJWP010000430          730 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.437, whole genome shotgun sequence.
ACCESSION   DAMJWP010000430 DAMJWP010000000
VERSION     DAMJWP010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.437"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            168..674
                     /locus_tag="PC700_003718"
     CDS             168..674
                     /locus_tag="PC700_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HDF2582930.1"
                     /translation="MGFREVKIEELQFNPFTKIGKEWLLITAGDSEKFNTMTASWGGV
                     GVYWGKNVVTTYIRPQRYTKEFVDSNDTFTVAFFDETYREALNICGTISGRDINKIEK
                     AGLTPYFVDDTVAFEEANMIIVCKKLYHGNMPPENFDAKENDKKWYPEKDYHTMYISE
                     IIKVLVKE"
BASE COUNT          293 a           73 c          130 g          234 t
ORIGIN      
        1 acacttataa aatatgaaaa ctacttattg cgaaagtaat tgatttgttg tattataatt
       61 tacatgaatt atgtaggaca atcaaaagtc agtattataa gagtacatat attcaaattt
      121 ttaacaatta atttatataa ttaaaaaatg aagggtagga tgaagtcatg ggttttagag
      181 aagtaaaaat tgaggaacta cagtttaatc catttactaa gattggcaaa gaatggttac
      241 ttataacagc tggtgattca gaaaagttta acacaatgac tgctagctgg ggtggtgttg
      301 gagtctattg gggtaaaaat gttgttacaa cttacataag accacagaga tatacaaaag
      361 aatttgtaga tagtaatgat acatttacag ttgccttctt tgatgaaacg tatagagagg
      421 ctttaaatat ttgtggtact atttctggta gagatataaa taaaatagaa aaagcaggac
      481 ttacaccata ttttgtggat gatacggtag cttttgaaga agctaatatg attatagtat
      541 gtaaaaagct atatcatggt aatatgccac cagaaaactt tgatgctaaa gaaaatgata
      601 aaaaatggta tccagagaaa gactatcata ctatgtacat ttcagaaatt ataaaagttc
      661 ttgtaaaaga ataattataa taaaaaatat atgaaaagaa cttattataa gtaaatgact
      721 gtgctttctt
//
LOCUS       DAMJWP010000431          723 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.438, whole genome shotgun sequence.
ACCESSION   DAMJWP010000431 DAMJWP010000000
VERSION     DAMJWP010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.438"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..>723
                     /locus_tag="PC700_003719"
     CDS             <1..>723
                     /locus_tag="PC700_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HDF2582931.1"
                     /translation="KNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGD
                     STTMYPSQNISDEIKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRT
                     VPYISKVSGVPIVDLATKCMLGAKLKDLGYGTGVYKEPKLVSVKVPVFSMSKLSKVEV
                     SLGPEMKSTGEVLGVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAK
                     DMKELGYTFVATEGTAKSLRENG"
BASE COUNT          292 a           75 c          154 g          202 t
ORIGIN      
        1 aaaaatccag tacttataga taaatatcta actggtagag aaatagaagt agatgctata
       61 tgtgataagg aagatatatt gatacctggt ataatggaac atttagaaag agctggagtt
      121 cactctggag atagtacaac aatgtatcca agtcaaaata tatctgatga aataaaagaa
      181 aagatagtag aatacactaa aaaaatagct ttagacctta atgtgcttgg tatggttaat
      241 atacaattta tagaatttca aaatgaatta tatataattg aggttaaccc tagagctagt
      301 agaacagttc catatataag taaagtaagt ggagtaccaa tagttgactt ggctactaag
      361 tgtatgttag gagctaaatt aaaggattta ggatatggta caggagttta caaagagcca
      421 aaattagtat cagtaaaagt accagtattc tcaatgtcta aattatctaa agttgaagta
      481 agtctaggac ctgaaatgaa gtctacagga gaagtcctag gtgtaggaga aaatttagaa
      541 gaagccttat ataaagggtt cttggcagca ggtagacata tgtctgatga aagaggagta
      601 gtacttgcta ctgtaaataa tcatgataaa gatgagttta tagagatagc aaaagatatg
      661 aaagaattag gatatacttt tgttgctaca gaaggaacag cgaagagttt aagagaaaat
      721 gga
//
LOCUS       DAMJWP010000432          721 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.439, whole genome shotgun sequence.
ACCESSION   DAMJWP010000432 DAMJWP010000000
VERSION     DAMJWP010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.439"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(123..>721)
                     /locus_tag="PC700_003720"
     CDS             complement(123..>721)
                     /locus_tag="PC700_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582932.1"
                     /translation="EIKFSIGSKLYLEIVNNKSKNGDIEGHDSYSNLIIYDIEDNTFN
                     ISNKFMDNLNKEKNANPKEIGLLSKTKLDYMIKNNKLNLIVTDMSNSIINLVYDINSN
                     EIKLEDYEILDLKVDYNEASSGVRKVKLLDDKIYSISGMKNSISGELKGFKGIVDVVG
                     TKPVKFQVFDMKLKKNIYEAELINGDISLQEKLFFVAN"
BASE COUNT          246 a          116 c           54 g          305 t
ORIGIN      
        1 atatttgatt tcatataaaa aaagtgtctc atgaactaaa aagtttattt tgagacactc
       61 ccttacttat acttatctgt gatacatata aaagtttaaa gttctaaaat taattctaat
      121 acttaatttg caacaaaaaa taacttttct tgtaaactta tatctccatt tattagctca
      181 gcttcataaa tattcttttt aagtttcata tcaaatactt gaaattttac tggcttagtt
      241 ccgactacat caacaatccc tttaaatcct tttaattctc ctgatatact gttcttcata
      301 cctgaaatag aataaatctt atcatccagt aacttaactt tcctaactcc agatgatgct
      361 tcattataat ctacttttaa atcaagtatc tcataatctt ctaatttaat ctcattagaa
      421 ttaatatcat aaacaagatt gattatggaa ttactcatat ccgtaactat aagattcaat
      481 ttattatttt ttatcatata atctagctta gttttactta ataagccaat ctcctttgga
      541 tttgcattct tttctttgtt tagattatcc ataaatttat tacttatatt aaaagtgtta
      601 tcttctatat catatataat aaggttagag taactatcat gtccttcaat atctccattt
      661 ttacttttat tatttactat ttctaagtat aatttacttc caatagaaaa tttaatttca
      721 t
//
LOCUS       DAMJWP010000433          715 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.440, whole genome shotgun sequence.
ACCESSION   DAMJWP010000433 DAMJWP010000000
VERSION     DAMJWP010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.440"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(22..>715)
                     /locus_tag="PC700_003721"
     CDS             complement(22..>715)
                     /locus_tag="PC700_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2582933.1"
                     /translation="EGKKSERVILNDYINTFFYNYNFEYNIKEFENGGDLLKKYPNNV
                     DIIFMDIDLNDENGFNVSKEIKKFDESVRIIFVSSSNKYIKKGYEIGVYRYLEKPLKY
                     SLFKEAISSYLKECYKNEAILSIKCKCKIYFIKVDDILYLEVLKNDLTIHTEKGIYTF
                     KNSMKEIESNLEEHNFFRCHKSYLINLMKVNMLDIENNIVFINQNSIIVSRYKIKLLR
                     EKLNSCLVDVII"
BASE COUNT          246 a          102 c           53 g          314 t
ORIGIN      
        1 atataagggc aaaaatttac ctcaaattat tacatctacc aaacaactat ttaacttttc
       61 tctcaataat tttatcttat aacgacttac aattattgaa ttttgattta taaatacaat
      121 attattttct atatctagca tatttacctt cattaaattt ataaggtagc ttttatgaca
      181 tctaaaaaaa ttatgttctt ctagatttga ttctatttct ttcatactat tcttaaaagt
      241 atatattcct ttttcagtat gtatagttaa atcatttttt aaaacttcta aatataaaat
      301 atcatctacc tttatgaaat atatcttgca tttacatttt atagataaaa tagcttcatt
      361 tttgtagcat tcttttaaat atgatgatat tgcttcttta aatagagagt attttaaggg
      421 cttctctaaa tatctataaa ctcctatctc ataacctttc tttatgtatt tatttgatga
      481 ggatacaaat ataattctta cactttcatc aaattttttg atttcttttg aaacattaaa
      541 tccattttca tcatttaagt ctatatccat aaatatgata tcaacattat taggatactt
      601 ttttaataaa tcgcctccat tttcaaactc tttgatattg tattcaaaat tgtaattata
      661 aaaaaaggta tttatataat catttagaat taccctctct gattttttac cttcg
//
LOCUS       DAMJWP010000434          714 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.441, whole genome shotgun sequence.
ACCESSION   DAMJWP010000434 DAMJWP010000000
VERSION     DAMJWP010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.441"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     regulatory      <1..89
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            172..657
                     /locus_tag="PC700_003722"
     CDS             172..657
                     /locus_tag="PC700_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDF2582934.1"
                     /translation="MISELMLKNFGKSSINFKTKIKLNLREKPDINSLKLKSIPEGKI
                     VKLKCVDGIWAEVESNYDKGWLLYKYLERLSNAKNNSNDTIKNRKKNYIGNIRTNGLS
                     LELRNDRTLESKVITTIPDGFKVEVCYSVGKWARVNINKNEKRYSGYVYNQYIEEHLS
                     L"
BASE COUNT          319 a           61 c          116 g          218 t
ORIGIN      
        1 atataggcaa tattagataa atctaatgac gcaaaactat agggactaaa atctttatgt
       61 aataaaggtc atgtcagcca gttgccaaaa agataataat tctttttgtt aaaagtattt
      121 ttagagttat atattttaat aacttaatat ttggcaagag gagggcttaa gatgatatct
      181 gaattaatgt taaaaaattt tggcaaatct agcatcaatt ttaaaactaa aattaaattg
      241 aatttaagag aaaaaccaga cataaatagt ttgaaattaa aaagtatacc agaaggtaaa
      301 attgttaagt taaaatgtgt agatggaata tgggcagaag ttgagagtaa ttatgacaaa
      361 ggctggttac tttataaata tttagaaaga ttaagtaatg ctaaaaacaa tagtaatgat
      421 acaataaaaa atagaaaaaa gaattacata ggaaatataa gaacaaatgg attaagttta
      481 gaacttagaa atgatagaac attagaatct aaagttataa ctacaatccc tgatggtttt
      541 aaagttgaag tatgttatag cgttggaaaa tgggcaagag ttaatatcaa taaaaatgaa
      601 aaacgatatt ctggatatgt atataatcaa tacatagaag agcatttaag tttataatac
      661 gatactagtg agctttaaat atgttaataa atcacaaaaa agaatcccat taat
//
LOCUS       DAMJWP010000435          700 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.442, whole genome shotgun sequence.
ACCESSION   DAMJWP010000435 DAMJWP010000000
VERSION     DAMJWP010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.442"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..423
                     /locus_tag="PC700_003723"
     CDS             <1..423
                     /locus_tag="PC700_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2582935.1"
                     /translation="TISKKYKMRSVNPLKAWVKEYNLHKEIKETPKGGLFMETRKTTL
                     DERVEIVKDCIEHDFNYNLIAKKYFVSYSQVRRWTLKYKENGIVGLEDRRGKAKKEDE
                     LTQLDEIKLELKIVKAEKHKLQLENAFLKKLKNIERGW"
     gene            495..>700
                     /locus_tag="PC700_003724"
     CDS             495..>700
                     /locus_tag="PC700_003724"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2582936.1"
                     /translation="MQLCEIAKVNRSGYYKWLNRDKSNLELENIKLATLITNIYEEKK
                     GVFCSLRMTLQLNREYKLNVNHKRV"
BASE COUNT          307 a           78 c          126 g          189 t
ORIGIN      
        1 actatctcaa aaaaatataa aatgaggtct gttaatccac taaaagcttg ggttaaggag
       61 tataatcttc ataaggagat aaaagaaacc ccgaagggag gactctttat ggagactaga
      121 aaaacaacat tagatgaaag ggttgaaatt gttaaagatt gtattgagca tgattttaat
      181 tacaatttaa tagcaaaaaa atactttgtt tcatacagcc aagttagaag atggactctt
      241 aaatataaag aaaatggtat agttggttta gaagaccgaa gaggtaaagc aaaaaaagaa
      301 gatgagttaa ctcaattaga tgaaataaag ttggaattga aaattgtaaa agcagaaaaa
      361 cacaaattac aactggagaa tgcattctta aaaaagttaa aaaacataga aagagggtgg
      421 taattacact atccaagaat gaaaataaat ataaagctat tcaatcctta ttcaatgaaa
      481 aaaaagcttc gcttatgcaa ttatgtgaaa tcgctaaagt aaatcgttct ggatactata
      541 aatggctcaa tcgagataaa agtaatttag aacttgaaaa cattaaatta gcaactttga
      601 taactaatat atatgaagaa aagaagggag ttttttgctc tttaaggatg accttgcagt
      661 taaacagaga gtataaactt aatgtcaatc acaagagagt
//
LOCUS       DAMJWP010000436          680 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.443, whole genome shotgun sequence.
ACCESSION   DAMJWP010000436 DAMJWP010000000
VERSION     DAMJWP010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.443"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
BASE COUNT          258 a           57 c          105 g          260 t
ORIGIN      
        1 aaaataataa gaggtagata cagttttaag ggaatacaaa agtttttaat taaactatgc
       61 ttgttcagat agatatttat ttaagaaaaa agactattaa aagcaatata caagaatgat
      121 atattagatt gattaaacaa gcataaatat tatgtaaaaa actttaagtt atagaattta
      181 aatccaatgt agatagatta cgttttttct tgcttttatt atggtataaa ttggtatcaa
      241 tattcaaaag taatatattt atgatataat aaaattatag aaattttgca gtgagctata
      301 tttgtgataa aatttggcgt aacagttgaa atataaggtg ttgagagtgc atgataagcg
      361 ttatcaattg cactattgct cgttcactgc aaatttagga gagttgtata cgtgtaagtg
      421 ttgaaaatac taagtttatt ttggggtttt atattaacta tatggaatgt aaatctcgga
      481 gaatttccta atgtggattt aataggaagt gttttatatt aactatatgg aatgtaaatt
      541 ttagcaaatt tttcgtaaaa gtcactattt tcaatggttt tatattaact atatggaatg
      601 taaatacata taaaactttc atcttctgtt aattcattaa aacgttttat attaactata
      661 tggaatgtaa atccatcttt
//
LOCUS       DAMJWP010000437          652 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.444, whole genome shotgun sequence.
ACCESSION   DAMJWP010000437 DAMJWP010000000
VERSION     DAMJWP010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.444"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(50..427)
                     /locus_tag="PC700_003725"
     CDS             complement(50..427)
                     /locus_tag="PC700_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2582937.1"
                     /translation="MTITKIPPAELKVMKFIWSSDFTVASKDVIEAMEKLYNWKQTTT
                     LTLLSRLVNKQFLSARKIDRHTHYTVLIGEEEYLHFETKTFLDNMHGSSIESFMKSLF
                     KKGVNEEELNSVEKWVKDIKKDE"
BASE COUNT          233 a          101 c           53 g          265 t
ORIGIN      
        1 actaattatt gtaaaaacta tttattttat gataattaac tacaccaaat tattcatctt
       61 ttttaatatc tttaacccat ttttcaacag agttcaattc ttcttcattt accccctttt
      121 tgaacaagga tttcataaaa ctctcaatag aacttccatg catattatct aaaaaagttt
      181 ttgtttcaaa atgtaaatac tcttcttctc caataagaac tgtataatgt gtatgtctat
      241 caatctttct tgcacttaag aattgcttat ttactaatct tgataaaagt gttagtgttg
      301 tagtttgctt ccaattatat agtttttcca tagcttcaat gacatctttt gatgctactg
      361 taaaatctga actccaaata aatttcatga cttttaattc tgctggtggt atcttagtaa
      421 tagtcatatg attttacact cctctcaaac ttctacataa ttaaatacta tattaataga
      481 cttttcgagt caaaggtttt atttaaaata tctcatccaa atttattaat acaaaaaatt
      541 tatattttta ttcaaaatta aacacacaat ctattaatat aaagataaat atctattcta
      601 taagtataac agattatcaa attaaaattc taatatttga cataatatat ac
//
LOCUS       DAMJWP010000438          616 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.445, whole genome shotgun sequence.
ACCESSION   DAMJWP010000438 DAMJWP010000000
VERSION     DAMJWP010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.445"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(181..357)
                     /locus_tag="PC700_003726"
     CDS             complement(181..357)
                     /locus_tag="PC700_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582938.1"
                     /translation="MFRDKMDKCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGEKVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(411..500)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT          238 a           93 c           56 g          229 t
ORIGIN      
        1 ttgtgtatta ccacaaatac tattatatca tagattaaca taaatgtcta tttaattata
       61 atttttagtt aacattttta tttattctta atatgaaaat aaatttaaac attttatatt
      121 accaatatat taatgtaaat gtagataaaa ctaaaaaggg ttttctagaa gaaaatctgt
      181 ctaattatac ttacttacca ataccatcac ttgatgcaag caagattcca caactttttc
      241 tccgtactct aatataaaat catctaactg tgtatctata aaatcacaat aatcatatga
      301 actaccaata taagcagtta gcatatgtgt acatttatcc attttatctc taaacataat
      361 gtatttatcc ccctaaaaaa ctttcatgaa aaacaaaaag aaaatatctt tttggcaact
      421 ggctgacata acttatttta taagcgttag tccctatagc tttgcgtcac taaatttctc
      481 tagttttgcc gatttagttt tattctacag attaataata caatagaatt agctattatt
      541 caacatatct agctgaaaaa ttaataaatt tatatatgaa cgttaaatta tttgaaaaaa
      601 taaaaagccc ctctgt
//
LOCUS       DAMJWP010000439          614 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.446, whole genome shotgun sequence.
ACCESSION   DAMJWP010000439 DAMJWP010000000
VERSION     DAMJWP010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.446"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            87..560
                     /locus_tag="PC700_003727"
     CDS             87..560
                     /locus_tag="PC700_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2582939.1"
                     /translation="MFKEYLIRELKEDDIKNALDLIWNVFEEFESPDYCDEGITTFHQ
                     FIEYNSIIEKIKEKTLFFWGCFDSKKLIGVLAIRNLNHICMLFVDKNYHKHGVAKKLM
                     TTGIQYCICNKFNLTEITVNSSPYAVEVYHKLGFEDIAVEQIVDGIRFTPMKLLI"
BASE COUNT          249 a           54 c           81 g          230 t
ORIGIN      
        1 ttataaaaca aattgtattg aattaaattt atttaagata tataattata attataatta
       61 aaataataat aaaggtgttg tttataatgt tcaaagaata cttaataaga gaattaaaag
      121 aagatgatat taaaaatgca ttagacttaa tctggaatgt atttgaagaa tttgaatctc
      181 cagattattg tgatgaagga attacgacat ttcatcaatt tatagaatat aattctatta
      241 ttgaaaaaat caaagaaaaa acactcttct tttggggttg ttttgactct aaaaaactta
      301 ttggagtctt agctattaga aatttaaatc atatatgtat gttatttgta gataaaaact
      361 atcataaaca tggtgttgca aaaaaattaa tgactacagg catacaatac tgtatttgta
      421 ataaatttaa tttaactgaa ataactgtaa attcatcacc ttatgctgtt gaagtttatc
      481 ataaattagg atttgaagat attgctgttg agcaaattgt tgacggtata cgttttacac
      541 ctatgaaatt gttaatataa taaattaaat aaaaagcaaa gtctcttaaa tattttatag
      601 ttactttgct ttta
//
LOCUS       DAMJWP010000440          589 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.447, whole genome shotgun sequence.
ACCESSION   DAMJWP010000440 DAMJWP010000000
VERSION     DAMJWP010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.447"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            100..468
                     /locus_tag="PC700_003728"
     CDS             100..468
                     /locus_tag="PC700_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2582940.1"
                     /translation="MKKIPKSELLVMNFIWDKGIDKIPAREVANYMEISHGWTKGTTG
                     KVLSRLSDKGFLEFEKDGRNTLYTIKVKHEEYLKFETKDFLELIHKNSIHSFISSLGC
                     GNEISDDDIKELEQWLKKRK"
BASE COUNT          245 a           52 c           88 g          204 t
ORIGIN      
        1 aagcataaaa taatataaag attctttata catgatgata tattatattg actattagac
       61 atttgtctaa tataatcaag ttaaaggtga gtgattatat tgaaaaaaat accaaaatcc
      121 gagttgttag ttatgaattt catatgggat aaaggcatag ataaaatacc agccagagaa
      181 gtagctaatt atatggaaat ttctcatgga tggacaaaag gtactactgg taaagtactt
      241 tctagattaa gtgataaagg ttttttagaa tttgaaaaag atggtagaaa tactttatac
      301 actattaaag ttaaacatga ggaatatcta aaatttgaaa caaaggattt tttagagctt
      361 atacataaaa attctattca tagctttata tcatcattag gttgtggaaa tgaaatatca
      421 gatgatgaca taaaagaact tgaacaatgg ctaaaaaaaa gaaaatagga gacgtatttt
      481 atggaattat ttatagaagt tattaaattt aatatttaca taagtctact aattcttttt
      541 ttcattttat ttactaaaaa gataagcaaa tatactttta agtttaatt
//
LOCUS       DAMJWP010000441          584 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.448, whole genome shotgun sequence.
ACCESSION   DAMJWP010000441 DAMJWP010000000
VERSION     DAMJWP010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.448"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(83..562)
                     /locus_tag="PC700_003729"
     CDS             complement(83..562)
                     /locus_tag="PC700_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HDF2582941.1"
                     /translation="MPNKLIKNLLSGILQILFFLLGLVIVVGGFKSFMYLCFSGEATL
                     QGTISGILMFILGVSYFIIIKSLIEVLSSSEHSLFVKDNVKRFRIIGYLLLLNSIMEF
                     ISTFGTTGKGMRFLDLGFGFYFTVPVFVYFITSLMSFVIADGFVKAIKIKEDNDLTI"
BASE COUNT          233 a          102 c           46 g          203 t
ORIGIN      
        1 ataccctaat atattttatt ttaaattact agttaatcac tcttttctgg ttatttatct
       61 cctatatatt aaagtgtcac acctaaatag ttaaatcatt atcctctttt atctttattg
      121 cctttacaaa tccatctgca attacaaaac tcataagaga agttataaaa tatacaaata
      181 ctggaacagt aaaataaaat ccaaatccta aatctaggaa tctcattcct tttccagtag
      241 taccaaaagt tgaaataaac tccattatag agtttaaaag caaaagatat ccaataatcc
      301 taaatctttt tacattatct tttacaaata agctatgttc agaactactt aatacttcta
      361 tcaatgattt tataatgata aaatatgata cacctaaaat aaacattaat attccagaaa
      421 tagtgccttg tagagttgct tctccactaa aacagaggta cataaaactt ttaaatcctc
      481 ccacaacaat tactaaccct aaaagaaaaa ataaaatttg taatatacca cttagtaaat
      541 tttttataag tttattaggc atcataccaa ctcctttaaa atat
//
LOCUS       DAMJWP010000442          575 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.449, whole genome shotgun sequence.
ACCESSION   DAMJWP010000442 DAMJWP010000000
VERSION     DAMJWP010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.449"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
BASE COUNT          228 a           43 c           73 g          231 t
ORIGIN      
        1 aatgtataag gtggtaaatt tatataaaaa agaatatgag ttgtaaaata ttaaataata
       61 ttgtactaat tggaataatt ttaactttat tatagattat tttaatgcca ttagtactga
      121 tggtgtattt taaaattggt ataagcatta caaatacgaa tatgccaact gtaatatgta
      181 atataagtag ctatatacat atgtgctgtc ccatatattg tatcattgtt tatattaaaa
      241 agattatgta aattggtatc aatcaataaa tcattttcag aagaaatacc taaaaactta
      301 aaaaatacct atatgtgtat ttagttagat tggagatttt tacatttaat tttatagtga
      361 caatattata ttattgttat gatgtctatt tatatgcaat tactataata tcagaactta
      421 tagtatcatt tgtttcatta tcaataaggt tttttacttt gttttgtcaa ttttattaaa
      481 aaaagttatt gatataaaag ataagaagga taaaactata tgacagattt caaacataag
      541 agtggatatt tatataatag atattttata gttaa
//
LOCUS       DAMJWP010000443          569 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.450, whole genome shotgun sequence.
ACCESSION   DAMJWP010000443 DAMJWP010000000
VERSION     DAMJWP010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.450"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(<1..461)
                     /locus_tag="PC700_003730"
     CDS             complement(<1..461)
                     /locus_tag="PC700_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate phosphodiesterase"
                     /protein_id="HDF2582942.1"
                     /translation="MKRFLRRIILVLFILLIFISFISIKLIHNVGDYGKLINYVGIVC
                     GASQRLTKLEMNHQPNDELIEYIDEILQELITGHGDYGLVITDCNEYNEDLLLLEKKW
                     EDLNIEIKKVRMKEQNNQLLSISEEFFSLANDTVFKIENFSKEKSNYLMTL"
BASE COUNT          202 a           99 c           41 g          227 t
ORIGIN      
        1 attaaagtca ttaagtaatt acttttttct ttagaaaagt tttctatttt aaacactgta
       61 tcatttgcaa gagaaaaaaa ctcttcactt atacttaata actggttatt ctgttctttc
      121 attcttactt ttttaatttc aatatttaaa tcttcccact ttttttctaa taaaagtaag
      181 tcctcattgt attcattaca atcagtgatt actaaaccat aatctccatg ccctgtaatc
      241 aactcctgta ggatttcatc aatatattct attaattcat catttggttg atgattcatc
      301 tctagtttag ttagacgttg gctagctcca caaacaatcc caacataatt aatcaatttt
      361 ccataatctc caacattatg aattaattta atagaaataa aacttataaa aatcaacaaa
      421 ataaataaaa ctaagataat tctcctcaaa aatctcttca taaaacacct ttatccccta
      481 tattgttttt tatgtttatt gaataccttc aaactatttt ttctaataat atacatgcgt
      541 ttacaataaa attttctata ctattagaa
//
LOCUS       DAMJWP010000444          558 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.451, whole genome shotgun sequence.
ACCESSION   DAMJWP010000444 DAMJWP010000000
VERSION     DAMJWP010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.451"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(<1..>558)
                     /locus_tag="PC700_003731"
     CDS             complement(<1..>558)
                     /locus_tag="PC700_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582943.1"
                     /translation="ICLSSVFIFTQLARVFINKKKINQKIKSRNGFRYDRDFIEARRE
                     EIHIKDNNNNKNKSNNKKLKEEKVFKYDNGDLYKGEFVDGKKNGFGIYIFSSKEKYEG
                     LWKDDKMHGIGKYTYRDGSIYTGEFKYGLKNGLGKLTYPNNDIYKGYFLDNKKNGKGV
                     LYKNDGNKQAGIWENDEQCKSLDFKD"
BASE COUNT          164 a          106 c           31 g          257 t
ORIGIN      
        1 atctttaaaa tctaaacttt tacattgttc atcattttcc catattcctg cttgtttatt
       61 tccatcattt ttatataata cacccttacc atttttctta ttatctaaaa aatatccctt
      121 ataaatgtca ttattgggat atgttaattt tcccaaacca ttttttaatc cgtatttaaa
      181 ttcacctgta tatattgaac catctctata agtatatttt cctattccat gcatcttgtc
      241 atctttccat aatccttcat acttttcctt acttgaaaaa atatatattc caaatccatt
      301 tttctttcca tcaacaaact cacctttata taaatcacca ttatcgtatt taaatacttt
      361 ttcttccttt aatttcttat tgttactctt atttttatta ttattgttat cctttatatg
      421 tatttcttct cttctggctt caataaaatc tctatcatac ctaaatccgt ttcttgattt
      481 aattttttga ttaatcttct tcttattaat aaatactcta gctagttgag taaatataaa
      541 tacagatgat aagcatat
//
LOCUS       DAMJWP010000445          536 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.452, whole genome shotgun sequence.
ACCESSION   DAMJWP010000445 DAMJWP010000000
VERSION     DAMJWP010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.452"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..>536
                     /locus_tag="PC700_003732"
     CDS             <1..>536
                     /locus_tag="PC700_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF2582944.1"
                     /translation="SIIEIWSCKKVFDYTSQSKTDLKKLNMYFLFSVFLIISSLYINI
                     GANSRILICIFVMFVFYKSNYKVNIYKCLVISFFYWLILIGINGLSMLLVATVSNLDN
                     MNVLLSGSLYRYEAIFLGKIMLGILLYVYKASFKNFKISKKEIYICIPMIANLLSFFV
                     LYDYIINLVKISPERRTA"
BASE COUNT          188 a           48 c           75 g          225 t
ORIGIN      
        1 cttctattat agaaatatgg tcatgtaaaa aagtttttga ttatactagt cagtcaaaga
       61 ccgacttaaa aaaactaaat atgtattttc tcttttctgt ttttcttatt atttcttctt
      121 tatatataaa tataggagct aatagtagaa tcttgatatg tatatttgta atgttcgttt
      181 tctataagtc taactataag gttaatatat ataaatgtct ggtaatctca tttttttatt
      241 ggttaatatt aataggtata aatggattaa gtatgttatt agtagcaaca gtaagcaatt
      301 tggataatat gaatgtttta cttagtggca gtttatatag atatgaagca atattcttgg
      361 gaaagataat gttaggtata ttactttatg tttataaggc ttcttttaaa aattttaaaa
      421 taagtaaaaa agagatatac atatgtattc ctatgatagc aaatttattg tcattttttg
      481 tactttatga ttatattatt aatcttgtaa aaatctctcc tgaaagaaga actgct
//
LOCUS       DAMJWP010000446          529 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.453, whole genome shotgun sequence.
ACCESSION   DAMJWP010000446 DAMJWP010000000
VERSION     DAMJWP010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.453"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            66..398
                     /locus_tag="PC700_003733"
     CDS             66..398
                     /locus_tag="PC700_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDF2582945.1"
                     /translation="MNTREMKEYRGFYNKKLSETDEFFRVFGSLDDKTYSEGAISKKN
                     KELMGLAISILSRCDECICYHIEGCINSGASKDEIIEAIKIGVIGGGSITYPNARFAI
                     KILEEFNI"
BASE COUNT          210 a           39 c           94 g          186 t
ORIGIN      
        1 gtatttaata ttgaaaatat aagtacagtg tgatactctt ataagaaaaa cataggaggt
       61 agaagatgaa tactagagaa atgaaagaat ataggggatt ttacaataaa aaattaagtg
      121 aaacagatga gttttttaga gtatttggaa gtcttgatga taagacttat tcagaagggg
      181 ctataagtaa aaaaaataag gaattaatgg gactagctat ttcaatttta agccgatgtg
      241 atgagtgtat ttgttatcat attgagggtt gtattaattc tggagcaagt aaagatgaaa
      301 ttatagaagc tataaaaata ggtgtaattg gtggcgggtc tattacatat ccaaacgcaa
      361 gatttgcaat aaaaatttta gaagaattta atatataatt ttgatacacg tagtttattt
      421 agactaataa taatattcta tttaagtctt tgaaattaag ctttattttc taagttcact
      481 tcttattaag tttttagaat tgcatatata aatataaatt tttaaataa
//
LOCUS       DAMJWP010000447          526 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.454, whole genome shotgun sequence.
ACCESSION   DAMJWP010000447 DAMJWP010000000
VERSION     DAMJWP010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.454"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(14..427)
                     /locus_tag="PC700_003734"
     CDS             complement(14..427)
                     /locus_tag="PC700_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2582946.1"
                     /translation="MELEITEKIEKQDEDTIFQGLLKYNLARLEDKNPVDLGIYIRDK
                     AGKIVAGFIGVTHGNWLSIKYLWVSEKLRYKGTGSQLLYKTEKIAKERGCKYVFLDTF
                     SFQAPKFYEKFGYHKVFSLENYPLTGKRYYYRKIL"
BASE COUNT          173 a           80 c           53 g          220 t
ORIGIN      
        1 atgattttta ttattatagt atttttctat aataatatct ctttccagtt agaggataat
       61 tttcaagtga aaatacttta tggtatccaa atttctcata aaatttaggt gcttgaaaac
      121 tgaatgtatc taaaaaaaca tatttacaac ctcgttcttt agcaattttt tcagtcttat
      181 ataaaagttg acttccagta cctttatatc taagcttctc actaacccat aagtatttta
      241 tagaaagcca attaccatga gtaacaccta taaaacctgc aacaatttta cctgctttat
      301 ctcgaatata tattcctaaa tccactgggt ttttatcttc aagtctagca agattatatt
      361 ttaataatcc ttgaaaaata gtatcttcat cttgtttttc tattttttct gtaatttcta
      421 attccatatt tgaactttct ccttgtataa tcttttgtat attggtattt tttaataaaa
      481 tcagtagaaa gaatatagtt taacattatt ctaataggca tttatg
//
LOCUS       DAMJWP010000448          496 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.455, whole genome shotgun sequence.
ACCESSION   DAMJWP010000448 DAMJWP010000000
VERSION     DAMJWP010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.455"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..>496
                     /locus_tag="PC700_003735"
     CDS             <1..>496
                     /locus_tag="PC700_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582947.1"
                     /translation="IKNLLYKKDDSLKVNRYFVVENNKYKLLLYESFKKEFGINSMDL
                     AQLKLNDFGLKYFKEVDLILKKAIAINPTDSKLYYAQACNYMNFDGFPGYSDTTYNAL
                     DSINNSLKYLDKNDSDYKKNASNIYNLKGVILMFHKRFDEAQQYLNKALYFNKDNEDP
                     KYNLN"
BASE COUNT          210 a           58 c           59 g          169 t
ORIGIN      
        1 ataaaaaacc ttctttataa aaaagatgat tctctaaaag taaatagata ttttgtagtt
       61 gaaaataaca aatacaagct attattgtat gaaagtttca aaaaagaatt tggtattaat
      121 agtatggacc ttgctcaact taagcttaat gattttggac taaaatactt taaagaagtt
      181 gaccttatac taaaaaaagc tattgctatt aatcctactg attcaaagct ttattatgcc
      241 caagcatgta attatatgaa ttttgatgga tttcctggtt attcagatac aacatataat
      301 gctttagatt caattaataa ttctttaaag tatttagata aaaatgattc tgactacaaa
      361 aaaaatgcaa gtaatatata taacttaaaa ggagttattc ttatgtttca caaaagattt
      421 gatgaagcac aacaatattt aaataaagcc ttatacttta acaaagataa tgaagaccca
      481 aaatataatc taaata
//
LOCUS       DAMJWP010000449          481 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.456, whole genome shotgun sequence.
ACCESSION   DAMJWP010000449 DAMJWP010000000
VERSION     DAMJWP010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.456"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            222..>481
                     /locus_tag="PC700_003736"
     CDS             222..>481
                     /locus_tag="PC700_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2582948.1"
                     /translation="MNLQSKWVNFLKPTYVSDEIKRKKLQVIIFFSITYGLSYSLGLL
                     AICFNHLIDSEILAQFMMILPLSSVSIAKFYTEGRNNDKYNF"
BASE COUNT          196 a           40 c           62 g          183 t
ORIGIN      
        1 aatgattttt atataaattt taaacagaag aataaatttc taacgaaatt ggatatttct
       61 tctgtaaata catattcaaa aaaagaatta taagagttta gtcagcagtc gtgtaagtaa
      121 taaggacttt ataaatatat agaaatataa tgatatgagt ataagtagaa aaattttatg
      181 atttttacaa ttatttttta taaaaaggga ggttatgttt gttgaattta caatcaaaat
      241 gggtaaactt tttaaagcca acatatgtca gtgatgaaat aaaaagaaaa aaactacaag
      301 ttataatatt tttttctata acttatggtt tatcttattc actaggttta ttagcaatat
      361 gttttaatca tttaatagat tcagaaattt tagctcaatt tatgatgata ttgccattat
      421 cttcagtttc tattgcaaaa ttttacacag aaggaagaaa taatgataaa tataatttct
      481 a
//
LOCUS       DAMJWP010000450          443 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.457, whole genome shotgun sequence.
ACCESSION   DAMJWP010000450 DAMJWP010000000
VERSION     DAMJWP010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.457"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            257..421
                     /locus_tag="PC700_003737"
     CDS             257..421
                     /locus_tag="PC700_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582949.1"
                     /translation="MFGKPKESVRFLDFNDVAEKFGEKKAVAIFDAIAGKKSKEHIIK
                     DIEKLVKNEV"
BASE COUNT          180 a           31 c           74 g          158 t
ORIGIN      
        1 aaattcatag cagttttaat agataacttt ttcagataga ttaaacaaag ttttatttac
       61 tgtattctat tatcagtttc ctacaaatta aattgtctat ttttatttat agagtagaat
      121 aaactgtttt tgttaagaat tttgaaggta aagctagttt aaaaaaataa agggactaat
      181 ttgtctaacg tttattggtg aaaaataagt ctatagaagt tataataaat atataaaaat
      241 gtatgagagg tgttatatgt ttggtaaacc aaaagaaagt gttagatttt tagactttaa
      301 tgatgtagca gaaaagtttg gagagaaaaa ggctgttgca atttttgatg ccatagctgg
      361 aaaaaaatct aaagaacata ttatcaaaga tatagaaaaa ttagtaaaaa atgaagttta
      421 gatatttata aggtatattg agc
//
LOCUS       DAMJWP010000451          438 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.458, whole genome shotgun sequence.
ACCESSION   DAMJWP010000451 DAMJWP010000000
VERSION     DAMJWP010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.458"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(<1..403)
                     /locus_tag="PC700_003738"
     CDS             complement(<1..403)
                     /locus_tag="PC700_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2582950.1"
                     /translation="MHKPSYKTVFHSYIIFFMIFICLILTVIGIFLSLITIQKPNGEF
                     IRSDWPKKFTENFNEQIIFIDNKPQVKQSGLEKLRENQLWLQILDSSGNRVYGFLEPK
                     EHKDYYSNSELLTLANQNNSSNGTVYFNTIKN"
BASE COUNT          149 a           60 c           51 g          178 t
ORIGIN      
        1 tatttttaat tgtattaaag tatacagttc cattacttga attattttgg ttagctaatg
       61 ttaataattc agaattagaa taataatctt tatgctcttt tggttctaaa aacccataaa
      121 ctctatttcc actagaatct aaaatttgca accacaattg attttctcga agcttttcta
      181 atccagactg ttttacttgt ggcttattat ctataaatat gatttgttca ttaaagtttt
      241 ctgtaaattt tttcggccag tcactacgaa taaattcccc atttggtttc tgaatagtaa
      301 taagtgataa gaaaatacct attactgtca ggattaagca aataaaaatc atgaaaaaaa
      361 taatgtatga atgaaatact gttttataac taggtttatg catttaatca actcttttct
      421 taaactttta ttttagtt
//
LOCUS       DAMJWP010000452          428 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.459, whole genome shotgun sequence.
ACCESSION   DAMJWP010000452 DAMJWP010000000
VERSION     DAMJWP010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.459"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
BASE COUNT          160 a           52 c           37 g          179 t
ORIGIN      
        1 ttttaaatct aaaagctata ggttgaaaca tactcttttt attttgttta tttatagctt
       61 tagttattaa tcatatataa atctacaaaa cgtattccta gtaacttata ttttttgata
      121 tctacaatca taagactata ttattggatt catacctcaa aaatattgcc ttctgaacat
      181 tattatattc aaagttatgt taatttacaa tgatatatct aacatgtatt gattaaaaaa
      241 aattaaaact tgcttttcat ctcctatatc taaagaagtg gcatttagaa taaactattg
      301 taatattaaa gttgaaattt tctagtttat ttactctata gcaccatatt ttaatactat
      361 taagtaaaaa tgtagttaaa atatatacta atatccctga aataagtatt tcttttttat
      421 ttagcaca
//
LOCUS       DAMJWP010000453          420 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.460, whole genome shotgun sequence.
ACCESSION   DAMJWP010000453 DAMJWP010000000
VERSION     DAMJWP010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.460"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..>420
                     /locus_tag="PC700_003739"
     rRNA            <1..>420
                     /locus_tag="PC700_003739"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          106 a           97 c          130 g           87 t
ORIGIN      
        1 cgcctcccaa aaggtaacgg aggcgctcaa aggttctctc agtacggtcg gaaatcgtac
       61 gtagagtgta aaggcaaaag agagcttgat tgcaagacat acaggtcgag caaggatgaa
      121 aatcggactt agtgatccgg tggttctgcg tggaagggcc atcgctcaac ggataaaagc
      181 taccctgggg ataacaggct tatctccccc aagagtccac atcgacgggg aggtttggca
      241 cctcgatgtc ggctcatcac atcctggggc tgtagtaggt cccaagggtt gggctgttcg
      301 cccattaaag tggtacgcga gctgggttca gaacgtcgtg agacagttcg gtccctatcc
      361 gtcgcaggcg taggaaattt gagaagacct gtccttagta cgagaggacc gggatggacg
//
LOCUS       DAMJWP010000454          393 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.461, whole genome shotgun sequence.
ACCESSION   DAMJWP010000454 DAMJWP010000000
VERSION     DAMJWP010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.461"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(3..296)
                     /locus_tag="PC700_003740"
     CDS             complement(3..296)
                     /locus_tag="PC700_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582951.1"
                     /translation="MISMIMMLIGVLFIVFGIILTAVEIKGKMPGFYGVGQNAVRKNA
                     IGIIAAGIALIFFRNGLDSIDSILTYGVFLVIELVVIGLYFLIRIKYDKDGNL"
BASE COUNT          148 a           77 c           21 g          147 t
ORIGIN      
        1 ctttaaagat tcccatcttt atcatatttt attcttataa gaaaatataa tcctattact
       61 actaactcta tcactaaaaa tactccatag gtaagaattg aatcaatact atctaaccca
      121 tttctaaaaa aaattaatgc tatacctgct gctataatcc ctatagcatt ttttcttact
      181 gcattttgcc caactccata aaaacctggc atttttcctt ttatttcgac agcagttaaa
      241 attatcccaa acactataaa taatactcca ataagcatca taatcatact aatcatctaa
      301 ttacctcctt cacttaagga aatttcaatt tctaaaatct aaaataaata atatttattt
      361 tttataataa tattgtatat ccaattaact att
//
LOCUS       DAMJWP010000455          379 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.462, whole genome shotgun sequence.
ACCESSION   DAMJWP010000455 DAMJWP010000000
VERSION     DAMJWP010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.462"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..>379
                     /locus_tag="PC700_003741"
     CDS             <1..>379
                     /locus_tag="PC700_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2582952.1"
                     /translation="DYKNFLNYILLPIISLVYIYPFLCEEYGWRSFLQSILFDRFGKK
                     IGIVMVGTCWSLWHLPLQFTLYSPQTPIIGAIAHLIYGIGLSIFLGYVYMKTKNIWFC
                     SIMHVLINSLGLIFNDSEIIINYH"
BASE COUNT          120 a           43 c           56 g          160 t
ORIGIN      
        1 gattataaga atttcttgaa ttatattctt cttcctataa tatcattagt atatatttat
       61 ccttttttat gtgaagagta tggttggaga tcttttttac aaagtatttt atttgataga
      121 tttggcaaaa aaataggtat tgtaatggta ggcacttgtt ggagtttatg gcacttacct
      181 ttacaattta cactatatag tcctcaaact ccaataattg gagctatagc ccatttgata
      241 tatggtattg gactttctat atttttaggt tatgtttata tgaaaactaa gaacatatgg
      301 ttttgctcta taatgcatgt tctaattaat agcttaggat taattttcaa tgattctgaa
      361 ataattatta attaccata
//
LOCUS       DAMJWP010000456          373 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.463, whole genome shotgun sequence.
ACCESSION   DAMJWP010000456 DAMJWP010000000
VERSION     DAMJWP010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.463"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            177..293
                     /gene="rrf"
                     /locus_tag="PC700_003742"
     rRNA            177..293
                     /gene="rrf"
                     /locus_tag="PC700_003742"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            300..371
                     /locus_tag="PC700_003743"
     tRNA            300..371
                     /locus_tag="PC700_003743"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:332..334,aa:Asn,seq:gtt)
BASE COUNT          118 a           61 c           80 g          114 t
ORIGIN      
        1 tttgatgtta ttcattaaga tatatgtgca gtttttagag tgttaactct atgtaaagta
       61 ctaacttcaa ttgatgaagt atatatcgta aaaacaatta ctatttagta cttcaaaact
      121 attgacattt aaaaataaag atgttaatat aatacttgtc ttataaatag gcaatatgtg
      181 gttataatag caaagaggat acacctgttc ccattccgaa cacagaagtt aagctcttta
      241 gcgctgatgg tacttggtgg gaaactgcct gggagagtag gacgtagcca cgtgaatatt
      301 ccaaggtagc tcaatggtgg agcaaccggc tgttaaccgg taggctgtgg gttcgagccc
      361 cacccttgga gcc
//
LOCUS       DAMJWP010000457          366 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.464, whole genome shotgun sequence.
ACCESSION   DAMJWP010000457 DAMJWP010000000
VERSION     DAMJWP010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.464"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(101..217)
                     /gene="rrf"
                     /locus_tag="PC700_003744"
     rRNA            complement(101..217)
                     /gene="rrf"
                     /locus_tag="PC700_003744"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(226..299)
                     /locus_tag="PC700_003745"
     tRNA            complement(226..299)
                     /locus_tag="PC700_003745"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(265..267),aa:Gly,seq:tcc)
BASE COUNT          128 a           76 c           57 g          105 t
ORIGIN      
        1 atcttcacta tatcatttat tgccaaaaaa atctataaaa gacaaataaa aacatataac
       61 aattgtaggt ttattaaatt taatacaaca aaaaaagatt acgtggctac gtcctactct
      121 cccaggcagt ttcccaccaa gtaccatcag cactagagag cttaacttct gtgttcggaa
      181 tgggaacagg tgtatcctct ctgctattgt aaccacataa tcttttggag cgggtgaagg
      241 ggatcgaacc ctcacagctg gcttggaagg ccagaactct accattgagc tacacccgca
      301 ttaaattttt ataaattagt aaaactaatt ttttagagtt agcaccctaa aaactacata
      361 tatatc
//
LOCUS       DAMJWP010000458          366 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.465, whole genome shotgun sequence.
ACCESSION   DAMJWP010000458 DAMJWP010000000
VERSION     DAMJWP010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.465"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            104..337
                     /locus_tag="PC700_003746"
     CDS             104..337
                     /locus_tag="PC700_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDF2582953.1"
                     /translation="MATTTKNRFYDYVQENLNKYVEVHSYFSSCPLYGEIIEADSLSI
                     TLCSRYIDESNPTLNETYTLYLPLSSIISIRTL"
BASE COUNT          138 a           46 c           39 g          143 t
ORIGIN      
        1 ttcatatcta tttgcttatt ttttaactta gtttctaata gaagtactca tatttatctt
       61 tttaaaagtt tcaactttaa taaaaaataa aggagttgat tttatggcta ctactacaaa
      121 aaaccgtttt tacgattatg ttcaagaaaa cttaaataaa tatgttgaag tacattctta
      181 ctttagcagt tgtcctctat atggtgaaat tatagaagca gatagcttat caattacact
      241 ttgctcaaga tatatagatg aatctaatcc aactttaaat gaaacttaca cattatattt
      301 accattaagt tcaataatat ctattagaac actataaaaa gttaattaaa tatgggttat
      361 ttatat
//
LOCUS       DAMJWP010000459          364 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.466, whole genome shotgun sequence.
ACCESSION   DAMJWP010000459 DAMJWP010000000
VERSION     DAMJWP010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.466"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            29..313
                     /locus_tag="PC700_003747"
     CDS             29..313
                     /locus_tag="PC700_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582954.1"
                     /translation="MLVYNKSFYPNDIFPRLDFSKIKKQLKLIDNDLSDFGRICIIEK
                     EHYTISVNSIGEINVYYDLEYENKVYRIVYEIEKLFKSQVGRFSISTYRN"
BASE COUNT          167 a           22 c           60 g          115 t
ORIGIN      
        1 taaatagaat aaataagggg tggatgaaat gcttgtatat aataaaagtt tttatcctaa
       61 tgacatattt ccaagattag atttttcaaa aataaaaaaa cagttaaaat tgatagataa
      121 tgacctgtca gattttggaa gaatatgtat aatagaaaaa gaacattata cgataagtgt
      181 aaacagtata ggtgaaataa atgtgtatta tgatttagag tacgaaaata aggtgtatag
      241 aatagtttat gagattgaaa agttatttaa atctcaagtt ggaaggttta gtatatctac
      301 atacagaaat tgataattaa aaaagtagaa attaaaaaac ttaatactaa agatatagat
      361 aaat
//
LOCUS       DAMJWP010000460          353 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.468, whole genome shotgun sequence.
ACCESSION   DAMJWP010000460 DAMJWP010000000
VERSION     DAMJWP010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.468"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            5..286
                     /locus_tag="PC700_003748"
     CDS             5..286
                     /locus_tag="PC700_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2582955.1"
                     /translation="MASKQTPRNNLEKFLRIRVLSHTGDKIHIKLPVNFAKRMIENNA
                     LDLFNGKDDVVDGKKVTEILVKAFDYDLTGEVVHLERKNGDIIKVSIVQ"
BASE COUNT          151 a           27 c           59 g          116 t
ORIGIN      
        1 tcgtatggca tcaaaacaaa caccaagaaa caatttagaa aagtttttaa gaataagagt
       61 cttatcacat acaggcgata aaatacatat aaagttacct gtaaattttg caaaaagaat
      121 gattgagaat aatgctttgg atttatttaa tggaaaagat gatgtagtag atggtaaaaa
      181 ggttactgaa atattagtca aagcatttga ctatgattta actggagaag tagttcactt
      241 ggagagaaaa aatggagata ttattaaagt ttcaattgta caatagaatt attttatatg
      301 aattttattt ttgaatagtt ataaataatt agaaatttgt aaaaaagtat aat
//
LOCUS       DAMJWP010000461          343 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.469, whole genome shotgun sequence.
ACCESSION   DAMJWP010000461 DAMJWP010000000
VERSION     DAMJWP010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.469"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..>343
                     /locus_tag="PC700_003749"
     rRNA            <1..>343
                     /locus_tag="PC700_003749"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           81 a           76 c           98 g           88 t
ORIGIN      
        1 tgcatggttg tcgtcagctc gtgtcgtgag atgttgggtt aagtcccgca acgagcgcaa
       61 cccttgtctt tagttgccat cattaagttg ggcactctag agagactgcc agggataacc
      121 tggaggaagg tggggatgac gtcaaatcat catgcccctt atgcttaggg ctacacacgt
      181 gctacaatgg gtagtacaga gggttgccaa gccgtaaggt ggagctaatc ccttaaagct
      241 actctcagtt cggattgtag gctgaaactc gcctacatga agctggagtt actagtaatc
      301 gcagatcaga atgctgcggt gaatgcgttc ccgggtcttg tac
//
LOCUS       DAMJWP010000462          341 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.470, whole genome shotgun sequence.
ACCESSION   DAMJWP010000462 DAMJWP010000000
VERSION     DAMJWP010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.470"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(113..313)
                     /locus_tag="PC700_003750"
     CDS             complement(113..313)
                     /locus_tag="PC700_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDF2582956.1"
                     /translation="MNNGIVKWFNNEKGFGFISMESGDDVFAHFSAIQTSGFKSLEEG
                     QKVSFDIVKGARGPQAENITIL"
BASE COUNT          115 a           57 c           32 g          137 t
ORIGIN      
        1 agactaagta ttatttttaa gtattttctt gttgaagtta ttttttcctt ttttacaaaa
       61 tgcgtaaata aaaaaaagac tcttaaaaga gtctttccga aaaatctata tattataata
      121 tagttatatt ttctgcttga ggacctctag cgcctttaac tatatcaaag cttacttttt
      181 gaccttcttc taatgattta aatcctgaag tttgtatagc tgagaaatga gcaaatacgt
      241 catctccact ttccattgat ataaatccaa atcctttttc attattaaac cattttacta
      301 ttccattatt cattaaaaat actcctatta acctatatca a
//
LOCUS       DAMJWP010000463          307 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.471, whole genome shotgun sequence.
ACCESSION   DAMJWP010000463 DAMJWP010000000
VERSION     DAMJWP010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.471"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(<1..146)
                     /locus_tag="PC700_003751"
     CDS             complement(<1..146)
                     /locus_tag="PC700_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HDF2582957.1"
                     /translation="MTELMIISGLVLLICITSSKVLYKFGVPILLIFILLGMLFGSDG
                     MVGI"
BASE COUNT          123 a           52 c           24 g          108 t
ORIGIN      
        1 tagattccta ccattccatc agacccaaat aacatcccta gtaagataaa aatcaataat
       61 attggtactc caaatttata caataccttg ctagaagtta tacaaatcaa tagtactaac
      121 ccacttataa tcattaattc tgtcatagca tcaactccat ttcatataaa ttttgtagat
      181 taatttgcat ctacaatttt attacttatt caaatataac atgacataaa ttttataagt
      241 tatggtacat aaagttcaac aaaaatttca caatacatta aaatgtgagt gttatactta
      301 aatttat
//
LOCUS       DAMJWP010000464          298 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.472, whole genome shotgun sequence.
ACCESSION   DAMJWP010000464 DAMJWP010000000
VERSION     DAMJWP010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.472"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
BASE COUNT          120 a           34 c           36 g          108 t
ORIGIN      
        1 agacagtttt acatatttca actgtctcct aagaggggta tagttcacaa ctaagagtgg
       61 ctgggaactt aatataaagt tccagaaaag taaatcttca aaataattta ttgaaggaaa
      121 catcactcaa agttaagtaa aatatgtttc cttgctttta ttatatcaca aattaaaaat
      181 attaacacaa aattttgtga atatatactt attattgttc taaaaaaata tgtaaataat
      241 tgatttgatt tcatagaatt attgttgtaa tatttagtta aatatacaca atctcaat
//
LOCUS       DAMJWP010000465          296 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.473, whole genome shotgun sequence.
ACCESSION   DAMJWP010000465 DAMJWP010000000
VERSION     DAMJWP010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.473"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
BASE COUNT          129 a           28 c           31 g          108 t
ORIGIN      
        1 tgtcattaaa tatgccaata gttttatata ttattgattt tattaaaaaa agagatatac
       61 aaactaataa gttgtaatct ctcaaatata aaaaaacaat aaattaactc attggatttt
      121 ttagtccata atataattat agattattca acaaacaatc tatagaaatt gtggtattaa
      181 tgtttaaata accaactttt tctaaaataa cctttatagg aatatatgag ttcataagta
      241 aagatagcga aataagaaac cagtttggta tgtaaaatct agatttttaa ttaaat
//
LOCUS       DAMJWP010000466          264 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.474, whole genome shotgun sequence.
ACCESSION   DAMJWP010000466 DAMJWP010000000
VERSION     DAMJWP010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..264
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.474"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
BASE COUNT          133 a           18 c           33 g           80 t
ORIGIN      
        1 tagaatatat attagtttta tgaaataaat aggtttactt agcattaagt tttaaaaatt
       61 aaatataaag aatacatgat aaataaaaaa tttaaaaaaa gtaaaaaatt atattgacta
      121 gaatagataa agatgatata gtattacttg tccttgaaaa aaggaaaaag aaataaacac
      181 gaacattgaa aattaaacag taggttaatt tatattaaga aacaaaccat aaagccagat
      241 attttgataa caatagtatc tgag
//
LOCUS       DAMJWP010000467          253 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.denovo.475, whole genome shotgun sequence.
ACCESSION   DAMJWP010000467 DAMJWP010000000
VERSION     DAMJWP010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.denovo.475"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..>253
                     /locus_tag="PC700_003752"
     CDS             <1..>253
                     /locus_tag="PC700_003752"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HDF2582958.1"
                     /translation="LDLGDRSIVSYVIGKSNNNNLVFETFNKAIEVYPNAKPIFHSDR
                     GYQYTSRVFKSKLLTQGMIQSMSRVGHCIDNAPMEGFWGI"
BASE COUNT           92 a           32 c           49 g           80 t
ORIGIN      
        1 ctggatttag gtgatagaag catagtttca tatgttattg gaaaatcaaa taacaataac
       61 ttagtattcg aaacttttaa taaagcaata gaagtatatc caaatgcaaa acctatcttt
      121 catagtgata gaggctatca gtatactagc agggtgttta aatcaaaact tttaacacaa
      181 ggcatgatac aaagtatgtc gagagttggt cattgtattg acaatgctcc tatggaagga
      241 ttttggggaa ttt
//
LOCUS       DAMJWP010000468        35408 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.guided.013, whole genome shotgun sequence.
ACCESSION   DAMJWP010000468 DAMJWP010000000
VERSION     DAMJWP010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35408
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.guided.013"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            247..864
                     /locus_tag="PC700_003753"
     CDS             247..864
                     /locus_tag="PC700_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDF2582959.1"
                     /translation="MCKKTYLLVVSILIAGLLTACSGSPTKDSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIITPQQAMEIALKEQNG
                     MVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            972..3503
                     /locus_tag="PC700_003754"
     CDS             972..3503
                     /locus_tag="PC700_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF2582960.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVKEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLHSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            3937..4638
                     /gene="vanR"
                     /locus_tag="PC700_003755"
     CDS             3937..4638
                     /gene="vanR"
                     /locus_tag="PC700_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanR-ABDEGLN family response regulator
                     transcription factor"
                     /protein_id="HDF2582961.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KDLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            4688..5770
                     /locus_tag="PC700_003756"
     CDS             4688..5770
                     /locus_tag="PC700_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2582962.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIVGNL"
     gene            6031..7131
                     /gene="vanG"
                     /locus_tag="PC700_003757"
     CDS             6031..7131
                     /gene="vanG"
                     /locus_tag="PC700_003757"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HDF2582963.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            7128..7934
                     /locus_tag="PC700_003758"
     CDS             7128..7934
                     /locus_tag="PC700_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF2582964.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            7952..10090
                     /gene="vanT"
                     /locus_tag="PC700_003759"
     CDS             7952..10090
                     /gene="vanT"
                     /locus_tag="PC700_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HDF2582965.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            10404..11024
                     /locus_tag="PC700_003760"
     CDS             10404..11024
                     /locus_tag="PC700_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDF2582966.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(11190..11726)
                     /locus_tag="PC700_003761"
     CDS             complement(11190..11726)
                     /locus_tag="PC700_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDF2582967.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(11839..12513)
                     /locus_tag="PC700_003762"
     CDS             complement(11839..12513)
                     /locus_tag="PC700_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDF2582968.1"
                     /translation="MSLFIISQCITSFAFTPENNKFKVKPLPYAYDALEPYIDKETMK
                     LHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVRNNAGGAYNHK
                     FFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWAWLVATKDGKL
                     SIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVVNWNGALENYK
                     NLKSQD"
     gene            12716..14590
                     /gene="asnB"
                     /locus_tag="PC700_003763"
     CDS             12716..14590
                     /gene="asnB"
                     /locus_tag="PC700_003763"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDF2582969.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLREAFREIVPEYVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            complement(14682..16118)
                     /locus_tag="PC700_003764"
     CDS             complement(14682..16118)
                     /locus_tag="PC700_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDF2582970.1"
                     /translation="MICTIKPLKISIKKSTEPLIEFISDTTYKNKSSSYIKEVHFTRY
                     IGVDDSIDKYIEKVKNIDTYFFNNPTIPYIRLNNLKINFDKEQTDKMLKIFNIYDSSK
                     FNSWDLYNIDFPCKIENDTLNWTKKIAFKNTLDLFSTSMPNCTSSIIKNFAVKLLCWM
                     DYFLPKLFYNKVKTTISPKIVYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDLYP
                     ECSKFSTLFELSRKTPNILEIPFERHITNINYSENTNKTSPTTNKPNSKNTLNNTNLT
                     SKKIAQDEEVELSYEDLAKLSASVVMIVVCNNENKPFKSGSGVVINNEGYILTNLHVV
                     NDGYSFLVRFENDDKVYTSYQIIKYHSDYDLAVIKVDRKCKPIPVKVSKKPVRGQKIV
                     AIGSPLGLFNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLISAG
                     YDDGQNLNLAVESNLVKIFANNFIEIVN"
     gene            16447..17025
                     /locus_tag="PC700_003765"
     CDS             16447..17025
                     /locus_tag="PC700_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2582971.1"
                     /translation="MGITLSKGQKVDLTKGNPGLKNILVGLGWDTNKYDGGFDFDLDT
                     AAFLTGASGSVTNDGDFVFYNNLKHTSGAVEHLGDNRTGEGDGDDEQIVVDLSKIPGE
                     ISKISFTVTIHDATERRQNFGQVSNSYIRIVDKDTNEELIKYELGEDFSIETAIVVAE
                     IYKHNGEWKFNALGSGFEGGLAALCGNFGINL"
     gene            17064..17642
                     /locus_tag="PC700_003766"
     CDS             17064..17642
                     /locus_tag="PC700_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2582972.1"
                     /translation="MAIVLKKGQKIDLTKGNPGLKNIKLGLGWDTNSFDSGYDYDLDV
                     SVFMVGESQRVEKDEDFIFYNNLRHPSGAVEHLGDNRTGEGDGDDEEILVNLETMPKH
                     IQKIAVTVTIYEAAERRQNFGQVSNSYIRVLNSENDEEILRYDLGEEFSIETAITVCE
                     IYKYNGEWKFSAVGAGFEGGLEALCRNYGLNV"
     gene            17728..18357
                     /locus_tag="PC700_003767"
     CDS             17728..18357
                     /locus_tag="PC700_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2582973.1"
                     /translation="MSINLSKGDKIDLKKSNPGLSNILVGLGWDPVQQSGGGFFKSLF
                     GGGQADIDCDASVFMLNQEGKLSGIKDLIYFGNLKSACKSVLHTGDNLTGEGTGDDEQ
                     ILVNLDKVPSNIHKLLFVVNIYNCVDRKQHFGMIENAYIRVEDQGNKKEIAKYNLSDN
                     YSEKTTLIVGAIYRKDGSWQFKAIGEGTKDAGLKEVMQNLDRIECAYGI"
     gene            18347..21106
                     /locus_tag="PC700_003768"
     CDS             18347..21106
                     /locus_tag="PC700_003768"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HDF2582974.1"
                     /translation="MEFNNDIKYYNVDKEEVTKDLSVNPEKGLSESEVKTRREKYGLN
                     EFTPKEEGSFWDDLKESLSEPMIVILIIAAVVSAVIGETHDAIGIVGAIAIGIAIGMI
                     TEGRSKKAAEALSKLTENIEVKVLRDGEVHQISKSELVPGDIVYIETGDMVPADGRLI
                     ESINLKIREDMLTGESDDVSKKCDVVVSMEEIESKGAVVVQEPIPAKQINMVFGGTLV
                     AYGRGALVVTSIGDNSEMGKIAQNLSETDEQTPLQIKLGNLGGLIAKVSSAVAGLLFI
                     FMVFQMISKNVLNVDMSGILPFLESIDPVKTAFTVCVALIVAAVPEGLPTMINMTLAI
                     TMQKMAKINALVTKKEACETIGSVSVICSDKTGTLTENRMTVEVAYVDGRYIESSEEE
                     INSYFEENCMINSTADVEHNDGDIKYLGSATECALLLYYKNVDYRQVRKNSNIVAQNP
                     FTSDSKRMTSVIGQDNHHVLLSKGAPEVLLELCSHIQRGKDIVPLTENIKHEILEEIK
                     KLQIKSMRTLGFAYKEISQAEEEAAVTAESDAMNVSAMENNLVFSGFVGIRDPLRKDV
                     IESVNTANKAGVSVKMLTGDNINTARAIGEELGLLKNNMRVVEASYIDTLDDEELKQE
                     IQSISIVARSKPDSKMRIVSALQKSGDVVAVTGDGINDAPALSKADVGIAMGISGTEV
                     SKNAADIILTDDSFSTIVKGIKWGRGIYDNFQRFVQFQLTVNVIAFLVAIISQVMGQE
                     MPFTTIQLLWVNIIMDGPPALALGLEPVRDHVLNRKPVDRKANIISKSMVYTIVLNAF
                     YITAILMLQSTFNFLGVDEAHKGTVMFALFAFSALFNAFNCREFNADSIFPNFTHNKL
                     ALQIIVLTGIAQVIFTQLFQDFFNSVAMDFMTWVKVLCVAASVIVVNEVVKCIIRLVK
                     PKKTA"
     gene            21193..22755
                     /locus_tag="PC700_003769"
     CDS             21193..22755
                     /locus_tag="PC700_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceG family protein"
                     /protein_id="HDF2582975.1"
                     /translation="MGDSKVFEKIFSSQSDRGNYTPSKGYLSYFISYIGLEDEVLYNL
                     EIFKTKQNIDSKKDIALFTDVIANPSDFDIINYFKSGLQKYRTSMEDVDINILGFEEI
                     DYKIKQAMDRVLKEEEKEFTNDRVKQNFIVKIMAWIKIYIGALDINKNEAPKVIFYGD
                     IKKHEVYLLLILYLAGFDVLYLNPNSKSNIDILKSERYNIEFEEANIIEEKISFEERV
                     ILGEKIDKSSVKKAFTVGAEASKRISEELLNDAGFIKPWQLQDRKIKNLLLSSTVDEI
                     SIYWNQPLKLRPGFKFNDAIVEAPNFLSKINGIYNDKNEYIKFLDLLRDSESSTFIEF
                     NGDVDRFSKAFTREAFSLSFLLDSKGVIDKNSVLNNKDYSISTLALNQQIMILEKVEE
                     LLEGSMFLNGLSGEDKIKGLFTVLHMDKKFVHMMNNFDYSLINPKLIIYMYKSIVFDK
                     EIVFLMLLLSKIGFDIIILCPGGENNIENVINNQLIDIHRLDKMVYDLKLNSLENDIP
                     LLKKIFGKRRRF"
     gene            22756..23892
                     /locus_tag="PC700_003770"
     CDS             22756..23892
                     /locus_tag="PC700_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDF2582976.1"
                     /translation="MGININSFSKDKNSVEVTENLPTAVQEENFDIMEYTNNKKNELR
                     KSKEVEALTSLIEVENPDTILQFGRKASEGVARVSDSLLNTIKLNRNEENSKMLVHLT
                     KIMDKFDLDDFQETKEPNFVQKLFKKANNAIEMMFQKYETLGGEVEKIQIELEQYERD
                     IALSNKQIGAMLNENFEFYNELQKYIVAGEMAIEEMDNEILPFFKQKSETSGDQMDVV
                     NYQELLKVYDMLNQRVYDLRIAENIAIQTIPMLRGMQHNNYGLIRKINSAFVVTLPVF
                     KQCLSQAILLKKQELQAKSLKALDDKTNELLLRNAQNVSTQSAQIARMAGTSSVQIET
                     LEKMYNTIKSGIDETMRIEENNRALIKDNTKRLEELNTTIIYNK"
     gene            23956..25032
                     /locus_tag="PC700_003771"
     CDS             23956..25032
                     /locus_tag="PC700_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HDF2582977.1"
                     /translation="MKYFDYICKDDLERIFLKEPEDFSAKTEKDVLKYALGAFLYVPA
                     TQYNMIYKSIIGDVKGVRPLAICLEDAVGVNGELEAIENLRLILKNISNESITNKDGI
                     PLIFVRIKDVEQLLRIKEIIIKNSHSITGILIPKANSELIENCIEALYSMNLQDMYVI
                     PIIETREFIYNEKKELSFTNLYNAILRHKSRILSIRVGITDILGMYGIRRDKNFSIYN
                     NLICSSFILDIITYLQRPELDIPISGGVSEFFDMTNKDIRNKYIEEILLDKYHGLIGK
                     TVIHPMQIQIVQALSTVSYEDFTDALDILDSTDSKYGVSKGVLGERMNETNPHFLWAK
                     KTLILSKIYGVLNKGVDYEELLKF"
     gene            25010..26248
                     /locus_tag="PC700_003772"
     CDS             25010..26248
                     /locus_tag="PC700_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HDF2582978.1"
                     /translation="MKNCLNFNCEIDIIDNPLNLELNDFLDVGVRNNSKRRFLFISKK
                     LGKHLACKPSEMDNLGKLMATIYNEKNKEESNGTVIAFAETGTAVGHSFFDYLCGDYE
                     FIHTTREQFDELENLEFLEEHSHATDQNLYFGMLNNFKFGDEIILVDDEITTANTCMN
                     IIRKIQSIYPKKKYTICSILNWVSDENLSKVTRLERELNCKIDFVYLFKGNFKFYIDE
                     DIISNYNDNYSITKLETISSDKVNLVVNHIYVDLEDYIGNKKYVKYTGRFGINRREQF
                     RLKEIVKRESKKITMASKESLKNEKEKILFLGTEEFMYIPMLFAKEKENQYDIYYHST
                     TRSPIINIDKKHYPIKSKFMLKSFYNIDIQNYIYNIDKHNFSKCYLFVELNKSENSYL
                     EFIDIIKKTSIKELTIVCCS"
     gene            26302..27390
                     /locus_tag="PC700_003773"
     CDS             26302..27390
                     /locus_tag="PC700_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HDF2582979.1"
                     /translation="MRDFSTYSKDDVIFLLKDISNLIEEEGNKERELKIQNGRHYSEM
                     IPIEYEVSEDYLNLYHEKLRENKDKLAFSIGVMCEKIIKKNGNDVVLVSLARAGTPIG
                     ILAKRYIRSKYNVSLPHYTISIIRDKGIDMNAIEYIVKNHPSSKIQFLDGWTGKGTIS
                     KELEKACDELNVKFKKHFDSTLAVLADPAGYSGLYGIREDFLIPSACLNSTVSGLVSR
                     TVLREDLIGKNDFHGAKFYRHLKDKDESMNYIETIEECFKNQFKNISDEVENWESDII
                     TRDGYFDVLNIKEKYNITDINFIKPGVGETTRVLLRRVPYKILVKDLNDKSLDHIFIL
                     AKEKNVEVEQMDLKAYKCCGIIKNMKDI"
     gene            27418..28209
                     /locus_tag="PC700_003774"
     CDS             27418..28209
                     /locus_tag="PC700_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HDF2582980.1"
                     /translation="MIVFSDLDRSIIYSNKFLNADSKYANIEIYREKEISYISLDTIN
                     LIKQIQYYGMFIPTTTRTVEQFKRIEFNKYGIYFPWSITSNGGVILKDNEILKSWSEK
                     IDKLKPDYEPIESMIHKFKDYLNVDGITNFKVAEDTFFYIVVDFSRFNLDSIKEYTNI
                     LESKNWKFYVSGRKIYFIPKEISKENAIKYLTKELGIEYFYAVGDSIMDYGMLNISNK
                     SYVLKHGDINKNEIENSFISSSFKGMSGTEEILSNILDENCLLNI"
     gene            complement(28324..28878)
                     /locus_tag="PC700_003775"
     CDS             complement(28324..28878)
                     /locus_tag="PC700_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDF2582981.1"
                     /translation="MNIDVGSKIRSLRKSKNISISTLAKNSDLSTGLISQIERNMVVP
                     SIVAMWKISKALDINIGYFFEEIGREDSDIVVKKNNRKKIVTNDSTKFYELLVPNLSG
                     KKIEFILVTLDGHTQKNSEFVTHEGEECGYIIKGKMKITLGNKEYLLEEGDSFYFNST
                     IPHVYENYDDEVCISVWAMTPPSF"
     gene            29073..30275
                     /locus_tag="PC700_003776"
     CDS             29073..30275
                     /locus_tag="PC700_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 domain-containing protein"
                     /protein_id="HDF2582982.1"
                     /translation="MKSFVSPDNIWVLWAVVTGWAAFSIYLEQKYNWASKVSGAIIAL
                     VGAMLLSNLNIIPVESVVYDQVWGYVVPLAIALLLYQCNIKKIWKESGRLLIIFLVGS
                     VGTVLGAMIGFLALKNVVPDLNIVAAMMTGSYIGGNVNFAAMSGAFDAPGELVSATVV
                     ADNLLMALYFFVLIAIPSIGFFRKHFKHPHVDEMESIGITEGETVAANYWGRKEISLK
                     DIAIAIGSAFIIVAVSVELSTWFKAIIPLSNPFLAMLNTLFGNQYLIITTLTMLFATF
                     MPNFFGDIKGAQELGTFLIYIFFVVVGVPASITLIIQKSPLLLLYCGIMVLINMLVTF
                     IVAKIFKFSLEEAILASNANIGGPTTAAAMAISKGWSKLVGPILIVGTFGYIVGNYFG
                     LLVGNILI"
     gene            30332..31312
                     /locus_tag="PC700_003777"
     CDS             30332..31312
                     /locus_tag="PC700_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDF2582983.1"
                     /translation="MIFDGHSDTWADVTIRRLNGEKDVFRRIHLDNFKRANIEGAIFV
                     IWIDPPYDKEPEKRVKQIISSIKDEIEDMKDVVNIVKKYSDIDKGISENKINILIGLE
                     GLSHIGRNIDLLDYFYKEVGARHASLTWNEQNELATGCLGDENRGLTEIGKKAVNKME
                     KLGMIVDVSHLNEKSFWDLMSVASKPVIASHSNARSLCDAKRNLTDNQLKEISNTGGL
                     VGINSFRGFTNSNPKKQDLIHLVDHIDYMVNLIGIEHVSFGFDFSDYLGKETIGSFTG
                     NASVPSVDGLESTHTAQNIVVELENRGYKKKDIELISSGNFYRLFKDILK"
     gene            31559..32512
                     /locus_tag="PC700_003778"
     CDS             31559..32512
                     /locus_tag="PC700_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDF2582984.1"
                     /translation="MKKRYLVIGIIILSYFSLFIGAEDINIMHIFAKNQHKLMIFIMS
                     RVPRLISILIAGVGMSIAGLIMQQISKNKFVSPTTGATIDAAQFGIVICMLLVPTASI
                     FTKTIIAFVFSLVGTFTFMKIIGKLQFKNIIFVPLVGIMFGNIIGSMTDFIAYKYDLS
                     QNVSSWMQGDFSMILKGNYEILYITIPLIILAYIYANKFTVVGMGMDFATNLGLSYKR
                     IVNIGLIIVALVTVCVVVTAGNIPFIGLIVPNIVSLYMGDNIRASIWYTGLLGAVFVL
                     ICDIFGRIIIYPYEISIGLTVGVIGSILFLYLILRRNVNEA"
     gene            32502..33458
                     /locus_tag="PC700_003779"
     CDS             32502..33458
                     /locus_tag="PC700_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDF2582985.1"
                     /translation="MKLSLNQKIYFVSILLFISIALFIIYGIDMNHLEYALSQRIPKI
                     FAMVLGGGCIAFTTVVFQTITNNQILTPSVLGLDSLYVMIQTIIVFIFGSSSSLIINE
                     NYNFIINVAMMIGASLLLYKMLFEKNKNNIFFLILVGMIFGTLFKSATTFIQVMIDPN
                     EFLALQTSIMASLNNINTNVLLIAFIIIIAIIPFIYDEIKYLDVLSLGKEQAINLGVD
                     FDKAVKKMIILIAILVSISTALIGPMTFLGLLLANITREIFKTYKHTYLISGSMLIGM
                     ITLIIGQFFIQHVFKFDTTLSVVINFIGGIYFIQLLLKGANR"
     gene            33455..34210
                     /locus_tag="PC700_003780"
     CDS             33455..34210
                     /locus_tag="PC700_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2582986.1"
                     /translation="MIEIKNIFKRYKNKNVVDDVSFSIEKGKITSFIGPNGAGKSTVL
                     SIVTRLIGGDGGEVIIEGKSLTNYSNKELAKKIAILKQSNNITLKLTIRELVGFGRFP
                     YSEGNLTKEDENYIDEAIEYMKLTDIQHKYLDELSGGQRQRAYIAMVIAQDTEYILLD
                     EPLNNLDMNHSVQMMKVLRSLCDELDKTIVLVMHDINFASCYSDNIVALKNGKVEKVG
                     RTDEIVNEKVLEDIYEMNFNIKNINGNRICIYF"
     gene            34302..35267
                     /locus_tag="PC700_003781"
     CDS             34302..35267
                     /locus_tag="PC700_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2582987.1"
                     /translation="MNKKAAIVAAVAIIGLVTVFALGGSKKNESKTSENSNNTIKITH
                     NLGETDVKLNPKKVVVFDYSALDTMDALGVAENLVGLPKTSLPASLEKYKDKKYADLG
                     GLKEPDLEGIKSANPDLIIINGRQEDFYEQLSKIAPTISTSKDDKKYLESVKNNIDKI
                     AKIFGVEEKANQEFSKIEKKIETLNKKVTDKNLNALTIMVNEGNLSVFGEESRFSILY
                     NSFGFENKDKNIKESSHGQNITFEYIAKQNPEVMFVIDRGIATGSDVKESSTAKSVLN
                     NDIIKSMDAYKNDNIIYLDSPTWYVNDGGLTSLNKMIDDASKAVN"
BASE COUNT        13778 a         3705 c         6134 g        11791 t
ORIGIN      
        1 tcaaaatgaa ctttttagtt catgaggcac tctttttgta cgaaataaaa tatattttta
       61 ttatttcaac aatgaaaaag atgcttgttt ctattttgag acagtataat ttttatgtaa
      121 ctttaatttt agtaaatata aagttttaaa attgataaaa taggaggaat ttttaatata
      181 aaagggtaaa taaagggttt agagggcata atatagaata aattaatata tttaaggagg
      241 atttttatgt gtaaaaaaac gtatttatta gtggtaagta tacttatagc aggtttatta
      301 actgcatgta gtgggagccc aactaaggat tcaggacaag cacaagataa tacaaaaaag
      361 gaggcaaatg cttcaaataa tgctttaaaa gatgaaaaaa ataatgaaaa tttaatggaa
      421 caagatttta aagtgccata cacagatgca ataaacatat ttaaagataa atacaaggat
      481 gctgatatag tagatttaag tcttgagaga gatttaaata aatttgttta tactgtggaa
      541 ggtgtagatg ataataatga atataagatg aaaattgatg ctaatacaaa agatgtatta
      601 gaagataaaa cagaaaagtt agattcagaa gatttaaatg gagttgctag aaaagaaaaa
      661 ttagatttaa atgacataat tacacctcaa caagctatgg aaattgcatt aaaagaacaa
      721 aatggaatgg ttaaagaatg gagtttagac aaagatttag atgttacttt ttataaaata
      781 agaatagata aagataaaaa tgaatatgac ataaaagtag attctaaaaa gggaactgta
      841 ttgaaggtag aaaaagaaga ttagaagatt taaactatct ttaaaataat ttcttttttg
      901 aataaatttt gtttaaaata taacaagcag ggtataaaga gactaaataa ctattttagg
      961 aggagtacgg tatgtcattg aaatcactaa aaataaaaga aaatttatat tgggtaggtt
     1021 cacttgaccc tgatttaaga gtatttgaca taattatgta tacaccttat ggaactactt
     1081 ataattcata tgtattaaaa ggaacagaaa aaacagtttt gtttgagaca gttaaagata
     1141 agcattttga caattatatt gaaagattaa atgatttaaa tatagatttt gaaaaaatag
     1201 actatattgt tgtaagtcat actgaaccag accatgcagg aagcgttgaa aagcttttgg
     1261 atttagctaa aaatgcaaaa gtagtagctt cagaaacagc aattaaatat cttaaggaaa
     1321 tcgtaaacaa agactttgaa tatgttgcag ttacagatgg agatacatta tcaattgggg
     1381 acaaaacttt agaatttttc tctgtaccta tgcttcattg gccagatact atatacacat
     1441 atataaaaga ggataaaact cttgtaacat gtgattcatt tggtagtcac tatagtaatg
     1501 ataagatagt aaatacactt gatgaaaatg aagaaaagga ttacttggat gctttaagat
     1561 actattatga ttgtataatg ggtccattta aaccatctat ggtaactgct atagaaaaga
     1621 taaaagattt agatatagat actgtatgtc caggtcatgg tccagtatta actgaaaatc
     1681 ctagaaaaat tatagacctt tattataact ggagtgtaaa ggaacaaata aagttagaaa
     1741 aagaagttac aatttgttat gtttctgcac atggatatac aaagattatg gcagaagcta
     1801 taaaagcata tattgaaaaa aatagcaact ataaagtaaa tttatttgat gtaatagagc
     1861 ataagcaaga agatattttg gcaaaaatag cagtatcaca aggtgtatta tttggaacac
     1921 caacaatatt aggagatgcg ttaaagccta tatgggatat attaatatcg ttaaacccag
     1981 ttcttcatgg tggaaaagtt gcttcagtat ttggttcata tggatggagt ggagaaggta
     2041 tagaaaatgc tatggagaga ataagtcagc ttagaatgac agcagtaaaa ccttttgctg
     2101 ttaactttaa accatcaaat gaagagattg ataaattaca ttcttataca ggtaaattcc
     2161 tagataagtt aaattctact tttggaagta aaaagaaaac taagaaattt aaatgtgtaa
     2221 tttgtaatga agtttttgaa ggtgatagtg ctcctagtgt atgccctgta tgtggagcta
     2281 aggaagacca atttatagaa gttgaagaag atgaagttac ttttagaaaa gatactgatg
     2341 aatatttcgt aatagtagga aatggagcag ctggttttta tgcagcagat gctataagaa
     2401 aaagaaataa aacttgtaag ataactatga tttctaatga agatgagttg acttactata
     2461 gacctgcact ttctgatggt ataaatgaag aacttggttc agatttctac atggaagata
     2521 aagattggta tgataaaaat aatatagtag taatattagg aacgaatgta gataaattag
     2581 atgaagtgaa taaaactatt atagtgaatg atggagctat taaatttgat aagcttgtaa
     2641 tagctacagg aagtagaaac tttataccac cgattaaggg ccatgattta gaaaatgtgt
     2701 ttactcttag aaatataaaa gatttatata gtgtaaaaga agcactagaa aaatctaaga
     2761 aagttgtagt gattggtggc ggtttattgg gacttgaagc tgcatgggaa tttagactaa
     2821 aaggattaga agtagtagta gttgaagcca tggatagcat actatcaaaa caacttgata
     2881 aagagggtag taaaatatta gaacaatgtg taagagatac aggaatagat gtaagattgg
     2941 gtgtagcagt tgatggtatt gaaggtgatg gaaaagctca aaaagttgta tttaaagatg
     3001 gagatagtgt tgactgtgat atggtagtat tttcaattgg tgtaagagca aacactcaaa
     3061 tggttcagga tacatctgta aaaattgata gaggtattgt tgtagataag acattacaga
     3121 caaatgttaa agatatatat gcttgtggag atgttgcaca agtgggaaat attagtttag
     3181 caatatggcc atcttcagta gaaatgggaa aaatagcagg agcaaatgca agtggagata
     3241 atttgacatt tgaaagtgaa gtatatccag tttcattaga tgcaatgaat gtaaaagtat
     3301 ttagtattgg taatatacaa aattttgata aagaaatttc atcaaaagat gaaggtcaaa
     3361 gaatatataa aaaattattt atgaaagatg gttcattagt tggagctata cttataaatg
     3421 atttaagctg tactgttaaa ttgataagat taatatctga aaaaggtgat tttgaggata
     3481 ttatgaaggc tgatatacta taatttaaat acattaaaat aaaaaggagt tgtctcaaaa
     3541 ttgatttttt tctaatcaaa ttttaaattc aactcctttt ttgaattttt atataaaata
     3601 acatggaaat tttagtaatg tctgtggtta catacatagg tttaattgtt ttttatatgg
     3661 attgtctctg gtataataaa ttgaattgtg tttgactgat agatattttt attgaaagtt
     3721 aatacctgtt atgcgataat aaggacgtgt taggggagtt tttataataa caattaatct
     3781 cagtttttgt gttccaatag tagcatcaac acattctgat gtagtagttc ttttcttata
     3841 aaaattagtt acaaatatgc ttgtaattat ttgatatgta aatttaccaa tattataaat
     3901 gatgacctcg ataataagga gagtgaaaaa taatctatga acgaaaatat tttgattgtg
     3961 gatgatgaaa aagaaatagc agatttaatt gaagtatatt taaaaaatga aggttttaca
     4021 gtatacaagt tttatacagg taaggaagct ctagaatgta ttgaatctaa agatttggat
     4081 atggctattt tagatattat gcttcctgat attgatggat ttcatatttg ccagaaaatt
     4141 cgagaaaaat actattatcc tatcattatg ttaacagcta aaattgaaga tatagacaag
     4201 ataatgggtt taaccatagg tgctgatgat tatataacaa agccatttaa ccctttggaa
     4261 gttgttgcaa gagtaaagac acaattaaga agatatatgc gatataataa ttcttatgaa
     4321 caacagtcga taattgttaa tgagtatgat attaaaggat tgattatcaa taaagaaaca
     4381 cataaatgta gtttatttgg gaaagaagta gcattaacac cgattgaatt ttctattctt
     4441 tggtatttat gtgaacatca aggaaaagtg gttccttctg aagaattgtt tgaagctgta
     4501 tggggagaaa aatatcttga taataataac acagttatgg ctcatatagg gcgattacgt
     4561 gaaaaaattc atgaaccttc taaaaacccg aaatttataa aaactgtatg gggagtaggt
     4621 tatacaattg aaaagtaaaa atattaaagt caatcctgac tttactcaag tgagaaataa
     4681 aatttttatg agaacagctt taatggcatt tatagccatt attttcatat ggattttata
     4741 ttcttttata ttgtatggta agttttctat ctggatagta tcattctttg aaaatgcttt
     4801 tcgtatggaa tataaagctg cattgaattt atatcagcag attttccgta atagcatgga
     4861 gctcttcatg atactagcta tggtgattgt gttttttata atattccgtg tttatttaaa
     4921 ttggtttaca aaatatttta gtgaaataaa tcaaggtatt gactctctta tcaaagaaga
     4981 tgttggagaa gtggctttat ctccagagtt attggcaata gaaaagaaaa taaactcaat
     5041 taaacatata cttgaacaac gtaaatttga gacacaaatg gcagaacaac gtaagaatga
     5101 acttattgtt tatcttgcac atgatttaaa aacaccactt acatcagtta tagggtatct
     5161 gacactactt cgagatgaaa gtcagatttc tgaagaactg cgaaaaaaat atttatctat
     5221 ttcgttggat aaggcagagc gtcttgaaga tttaattaat gaattctttg aaattacaag
     5281 gtttaattta tcaaatatat cacttgaata cagtacagta agcttaactc gtatgcttga
     5341 acaactaaca tatgagttta aacctacttt gatgagtaag aatttaaaat atacactgaa
     5401 tgttacacca aatatgatga taaggtgtga tgttaataaa atgcaacgtg tatttgataa
     5461 cttattacga aatgctgttt attatagttt tgaagatact actattgaaa taacagctgt
     5521 acaggaggat gattatataa agcttaaatt tataaatcat agcaatacga ttccagagga
     5581 aaaacttgag cgtatttttg aacaatttta caggttggat acatctcgta attcaaacag
     5641 tggtagagct gggttgggac ttgctatagc taaagaaata gtggagcttc ataacgggac
     5701 aataacagct catagcgaga acaatataat tacctttaag gttattattc cgattgtagg
     5761 aaatttgtaa gaatttaagt ataaaaaaat aggaaattct ttaggataaa taaataaaga
     5821 gcactcttgt tattgatata attactgtat caaaacaaga gtgcttttat tttgatacag
     5881 taattatatc aatagaaaaa tgtgaaaata tattatgtgg aacagatgca attgtaagtg
     5941 aaggttgatt ggatttgaaa tagaaaaagt attttaacat gtttctgtag attgtataaa
     6001 aataaataaa tcaacaaagg agaatcaaag atgaataata aaaaaattgc agttatattt
     6061 ggtggaaact caacagaata tgatgtatca ttacaatcag cttttgcagt acttgagaat
     6121 ttgaatacgg aaaaatatga tattatacca attggaatta caagggaggg taattggtat
     6181 cattatattg gagattataa taatatccag aataacactt ggtttgaaag ggatactgat
     6241 ataattccag ttattgtttc gcagaaccgt gtcaaaaatg gtattattga atttttaatt
     6301 cctgatgctg tttctcagaa tcatattcca aatagtgcta ttgaatcatg tgaaaatgat
     6361 attaaataca ctaaacttga ttttgtattt cctgtattgc atggaaaaaa tggagaagat
     6421 ggaacagtgc aggggttatt tgaattggca ggtatacagg ttattggatg tggtactctt
     6481 tcttcagcat tatgtatgga caaagacaaa tcacataaac ttgttcattc agaaggtatt
     6541 aaagtaccaa aatcaatatt actaaaatac tcagataaat ttaatgtggc tgaactgtca
     6601 aaagacttaa cttatccatt atttataaaa cctgtgcgtg caggttcatc atttggtatt
     6661 agtaaaattt ataaaaaaga agagttacaa gtagcagtag acttggcttt tgaacatgat
     6721 aatgaagtaa taattgagga aaatattgat ggttttgagg tagggtgtgc agttttagga
     6781 aacgatgact tacttgttgg aagagtagat gaaatagaat tgtcaaaagg attttttgat
     6841 tatacagaaa aatatacatt gaaaagttca aaaattcata tgccagctag aattgatttg
     6901 gattgtgaga aaagaataca agagacagca aaaattattt atagaactct tggatgttct
     6961 ggatttgcta gagtagatat gtttttaact ccatctggtg aaattatttt taatgaagtc
     7021 aatacaattc ctggctttac ttctcatagt cgttatccga atatgatgaa aggtattgga
     7081 ctgtcttttg aagaaatatt ggataagata attgaggtgt acataaaatg atgaataata
     7141 ttttattaga taaaaataaa gaagataaaa tgacaaatac tatcttattg actaaagata
     7201 aagtttatga tgggaatcta attctagtaa atgctttttt accagttaaa acatctgaag
     7261 atatagattt aattcctgta gatacaaggt ttccaagtat tcttatgaaa cgtgaagcta
     7321 caaatatttt acaaaatatt ttaaaaagta tatgtggaat aaatgaaata gtacctgtaa
     7381 gtggatatcg tactgcagag gaacaacaag atatatattc aagctctctg cgtgacaatg
     7441 gaaaagactt tactaaaaag tttgtagcac ttccaaatca cagtgaacat cagacagggc
     7501 ttgctattga tttagggttg aagaaagagg ttatagattt tatctgccct gaatttcctt
     7561 ataatggtat ttgtaatgag ttccgtaagg cagcactaca ttatggtttt atagagcgtt
     7621 atcagtgtaa aaaagaagaa ataacaggaa tatctcaaga accatggcat tttcgttatg
     7681 tcggatatcc acattctgag attatcaata aaatgaatct cgcccttgaa gaatatatag
     7741 aatatattaa gagattttcg tatacaaatt gtttaaaaat agaagataaa gaaaaaatta
     7801 tagaaatctt ttatgtgcct attcatacta aagaagaagt gagaatctct atacctgaac
     7861 aatgtgtata tcaaatatcg ggtaacaata aggatggttt tattgtgaca ttttggagaa
     7921 atagaaagga ctaagaaatg gaggaacaag aatgattagg aataaatctt atgcagggct
     7981 tgattatttt aggattatag ctgcattact tataattgca attcatacat ctccattatt
     8041 atcatatagt aaaatggctg actttgtgtt aactagtata attgctcgta ttgctgtgcc
     8101 attctttttt atgctatcag gcttttttct aatttcaaca tataattata attgtaataa
     8161 actgaaatct tttctaaaaa atgtattatt tatttacggg atagctattg taatttatat
     8221 tcccttaaat atttacactg gatatttttc catgaataat ttagtgccca atattattaa
     8281 ggacattgta tttgatggga ctttatatca tttatggtat ctacctgcat caattattgg
     8341 tgcaagtatt gcatactttc ttgtaaaaaa actaggttta aagtactcat tagtaatcac
     8401 tattgttatt tatgtaattg gtatgtttgg tgatagttat tatggttttt tagaaaaaat
     8461 accactatta aagaatttat atgggtatat ttttgaagta tcagattata cacgaaatgg
     8521 tatatttttt gcacctgtat ttttcatact tggtggaata atatctaata aatctaatta
     8581 tatgtcaact aaaaatagct tgattggatt tcttatatca ttcttactta tgttatgtga
     8641 gggaatgttt ttacataaac taggggtaca gcgtcatgat agcatgtata ttatgttatt
     8701 gccatgtatg tatttcttat ttacagcctt aacattttta aaaggttctc gagctaggtt
     8761 attgcgaaca tcatcattaa ttatttatat tatacatcct atgatgattg ttgttattcg
     8821 tatgttttct aaaatattcg gaatacaaac tgtcttggtt gacaatagcc ttattcatta
     8881 tatagtggta agtatagcat cttttatagc ttctataatt attatattga ttcttaaacc
     8941 tctaaaaaaa gacttgaaag gagaacataa ggtaaatacc aatagagctt ggattgaaat
     9001 tgatttaaat aatttggaac ataatgtaaa gatgatacaa aaagctatgc cagaagattg
     9061 tgaattgatg gcagttgtaa aagctaatgc ctatggtcat agtgcttttg aagtagcgac
     9121 ttgtgcaaac caaataggtg taaaagcatt tgcagtagca acaatagatg aggggattga
     9181 tttacgtcga tatggaatac taggagaaat attgattctt ggatatacta acccaataag
     9241 ggcaaaggaa cttcataaat acaatcttac acagagtgtt attgacctta atcatgctat
     9301 ctctttaaac aaacagaaat acaacataaa atcacacatt aaaattgata caggtatgca
     9361 cagactgggt tttgatgtta atgatgtgag gtcaatttta aaggcattta aattaaaata
     9421 cttagatata tgtggcatat ttacacactt gtgtgtttca gatagtctga aagatgaaga
     9481 tgtagacttt acaaataaac aaattgaatg tttttataag ttaattgatt ttctgttaaa
     9541 aaaaggtata aaaattccta aagtacatat tcaaagtagt tatggttttt ttaattatcc
     9601 caatcttaag tgtaattatg taagaattgg aattgcatta tacgggatat taagttctcc
     9661 aaatgatacg actaaattgc aacttgattt acgacctgtt ttatccttaa aatcacaagt
     9721 aattcttatt agaaaaatac aaaaagatga tagttttgga tatggaagag tctttattgc
     9781 aagcagagat agcgtaattg ctatattatc tattggctat gccgatggta tacctagaaa
     9841 tttatcctgt ggaaaaagtt acgtaataat aaatggttgt agagcagcaa tagtgggtcg
     9901 aatttgcatg gaccaacttg ctgttgatat tacagatata cctaatgtag aagttggtaa
     9961 tacggcaata ataataggaa gagataattt atctgaatta tctgcttctg aagttgcaaa
    10021 taactcatgt agtatatcga atgaattatt gagcagagta ggaagaaggt taaatgttat
    10081 aaagaagtaa ctcaattaaa tagtaatatt tgttgtactg tgtaatttat tttactctga
    10141 ctagacattt ttagtaatat tatgtctaat atatattttt tataacttac atgacaattt
    10201 aagttataaa aaggtgatgt ttcagatttt tattttgaaa catcaccttt tttgttataa
    10261 tatataataa aataattata ccttaactgt aaacttatat agattattta ctttttaata
    10321 ttagattcat tttttgagat tggtatataa taaacatata gagtatataa taatagttaa
    10381 agttaaaaaa ggggaaaagc aatatgcagt atatacaaga attacttgat ttgactcaaa
    10441 attattgggt gttagccaca ataatcggct tattaagtgc atttatagaa agctttatac
    10501 cagccttacc acttgttgct atagttacag caaatgctgc aatacaagga ttatttattg
    10561 gatgtttgtt atcatggatt ggctcaggac ttgggacaac ttcattattc ttattaataa
    10621 gcagatttaa tgatagtaag ctttttaata agttgagaaa tagtaaaact gaaaaagcaa
    10681 taagttggat ggataaacag ggatttaagt tattatttat agcgtatgcc tgtccattta
    10741 tgcctggttg tttagttact atcgcatcag cttttggtaa aaaagattta aaagattttg
    10801 tgccagcaat gttggcagga aaatttgtaa tgtttatagt tataagttac gtggcaagtg
    10861 atatagaagg ttttataaca agtcctttaa agatagcatc atttatatta ttggtgtttt
    10921 tgtcatggaa gattggaaat agagtaaata aaaatcttga aaatcataat tatgatttcc
    10981 atcataaaaa acatcacaat gataaagatg ataaaatgat ttagcttgta cgaaataaaa
    11041 cgtatcttaa aatgatttag tactaaatta ttttaagata cgttttattt tttgtattaa
    11101 ggtacaaaat ttttagttct tttgtaaact aaaatatata aaacttattt atacttagtt
    11161 caaaatattg ttatttattt tttctacaac tataataaaa tataaaattg atatccaatc
    11221 aaaaaaataa ctatgaaatt taatagctta gatatgaatt tttctgtttt tgagttagtt
    11281 tttataggta gtaaagaaat gtttatggta atgggataaa acaattcaat accttcttta
    11341 gtaataaggt caagacacaa gtgagataga tatcctatta taaacccact ggacaaacat
    11401 gagaaaacta tattattgtt agtgaagatt gtcagagatt taaaaaaact ataaataagt
    11461 aataaggcaa ttagactgtg agtaaaccct ctatgtctaa acctacttcc aaataatttg
    11521 tatattaaca taaatttttt acttatgtaa gaacctctca tatctatatc tggaaataaa
    11581 gaacctaaat atgcaaaata aatataaatt ataattaata ctattttata tgcaatatcg
    11641 tatttaacta ggtacagatt gtttataaag gggagtatca aaagtgcgaa tatataacct
    11701 cccctttcat gtgtttcttt tatcatataa aaatcctcct gaatcgtcac tataaacatt
    11761 atattggtaa atttagacta tcttttatta tattatatag caaacataag tttttttcaa
    11821 aaggatttta atatttaatt aatcttgaga ttttaaattt ttgtaatttt ctaaagcacc
    11881 attccaattt actacattaa accagttgtc aatgtattca tttcttctat tttgatattt
    11941 taaatagtaa gcatgctccc aaacatcaag tcctattata ggagttaggt ttttacttac
    12001 agggctatcc tgatttggag tagtcataat agataatttc ccatctttag tagctacaag
    12061 ccaagcccaa ccagaaccaa agacatctaa agcagatttt tggaactctt gcttaaattt
    12121 ttcaaaagaa ccaaagtctc tatcaatagc ttcttttaaa gattcagaag gtatggtttt
    12181 ttctggcgtc attatatcaa aaaagaattt atgattataa gctccacctg cattatttct
    12241 tacagttgta gcaatatctt taggtaaaga atctaaattt tgcaataatt cacataaaga
    12301 ataattataa agctcaggat atttttcaag agcagcattt aatttatcaa cataagcttg
    12361 ataatgctta tcatgatgca gtttcattgt ttctttatct atataaggtt caagtgcatc
    12421 atatgcataa ggtaatggtt taaccttaaa tttgttattt tcaggtgtaa aagcaaatga
    12481 agttatgcac tgtgagatta taaataaaga cataataact ggtattaata tttttttctt
    12541 cataaaaatc acctcctaat atagtatttc tagaaataaa aaaaatatga aatattaaca
    12601 gataatttat aagaatttaa tagcaatttc attttaaatt gaataagttt taaagccaaa
    12661 gacaaaacta tgaaatatag aataagacta aaggacagaa aggaattttg attatatgga
    12721 cggcaatatt tcaatttatt ataaaggtga tatatctaaa aaagaagata ttaaatttaa
    12781 aataaaagaa tctgtaaata atacaagact ttctagtaga atagagtata aggatgagga
    12841 aaaaactttt gattttgaaa gatttaatgt aattgatgct atttatggaa ataaaccatt
    12901 tgaaaaagat ggaaaagtta ttgtttttaa tggaaatata tataactatc atgaaataaa
    12961 agaagaatta attaaaaaag gacacaattt tactacaaat gatgatattg aagtcttatt
    13021 ggcatcttat atagaatttg gtaagaattg tgtacataag attaaaggaa tgttcaactt
    13081 tataatctat gatagagaaa atgaaagtat atttggagca agagatttat ttgggataaa
    13141 accactttat tatataaata aggaaaatgc aataattttt tcctcagagt ataagttttt
    13201 acttgaatat atgaaaaatt taaatataaa tgaaagaagc ttacaaagtt atttttcatt
    13261 tcaatatgta cttcctgaag atactatgat acaaggaata aggctgatac cagcaggtca
    13321 ctattttagg gtagaaaatg gtatactatc tttaaagaga tataataaac ttgagtttag
    13381 aagtagtaca aaattttttt atactaaaaa tcacttaggc aatagagatg tgaatgagga
    13441 tgatataaga aatatagtag tagattcaat agtaactcat atggaagagg agaaagaaat
    13501 tggaacattt ttgtctggtg gaatagattc atctattata actactgtgg cttcacaaat
    13561 taatccaaat ataaaatctt tttctgcagg ctttagtgtc aaaggatata gtgaattaga
    13621 agtagcaaag aaaacagctg ataagttagg tatagaaaat atccagatta atataacaca
    13681 agatgaatat ataaaatcat taccgaatgt aatttattct ttggatgacc ctatagcaga
    13741 cccttcagaa gtaggtcttt atttcttaat taaagaggca ggtaaacatg ttaaagtagc
    13801 attgtctgga gaaggagcag atgaattatt tggaggatat aatatatata aagagtatag
    13861 tactatgaaa tcagttgtca attcacctac ttatattaaa agtatattag gaagagtttc
    13921 agaacttatg ccaaacataa aaggaagaaa ttatctttat agagctacaa caccattaga
    13981 aaaaagatat ataggaaatg caaaagtttt tgaaaacagc gaagtaaaaa gattcttttt
    14041 taagtataag gaaaagaata tatatgaata cctgttgtct aatttgtata gagatgcaca
    14101 aaaaaataat tacgattata taagtaaaat gcaacatata gatgtgaata catggcttca
    14161 aggcgatata cttcaaaaaa ttagcaaact ttctacagct gagcaggttg aacttagagt
    14221 accattttta tataaagatg tatttgatgt agcaaaaaat ttaagaatgg agcaaaaaat
    14281 aaataagaat aatacaaaag ttttacttag agaggcgttt agagaaatag tcccagaata
    14341 tgttgttcaa aggaaaaaat taggatttcc aactccaata agagtttggt taaaagattc
    14401 tctaggggat attgtaaagg aaactattta taattcaaat gtagatgaat ttatagataa
    14461 aaaatatgca ataaaacttt tagatacgca tcttaaaggt catagagata attcgagaaa
    14521 aatatggact atatttacat tttgtctatg gcatcaatta tttattgagc ataaaaatgt
    14581 cgagtattaa tacttttttc aatattaatc agagggcaaa agcatgcaat gttttcaccc
    14641 tcttgattat tatggaatag gataagtaga attttgattg attagtttac tatctcaata
    14701 aaattattgg caaatatttt gacaagatta ctttcaacag ctaaatttaa gttttgtcca
    14761 tcatcatatc cagcggaaat caatcctagt aagtttccaa acatatctaa aagagctcct
    14821 cctgaactac cactagagat tggagctgta aattgtatca tttgaaccgt ttcaaaatca
    14881 cgaaaagctg agataattcc atctgaaact gtattaaaaa gacctaaagg gcttccaatt
    14941 gcaactattt tttgacctct cacaggtttt ttactgactt taacaggtat tggtttgcat
    15001 tttctatcaa cctttatcac agctaaatca taatctgaat ggtattttat aatttggtaa
    15061 gatgtataaa ctttatcatc attttcaaat ctaactaaaa atgaataccc atcattgact
    15121 acatgaagat tcgtaagtat ataaccttca ttatttatta ctactccaga accactctta
    15181 aacggtttat tttcgttatt gcacacaaca atcataacaa cagaagctga taactttgca
    15241 aggtcttcat aagaaagttc tacttcctca tcttgtgcaa tttttttaga tgttagattt
    15301 gtgttattaa gtgtattttt agagttaggt ttattcgtag taggagaagt cttattggta
    15361 ttttcactgt aatttatatt tgtaatatgc ctttcaaaag gtatttctaa tatatttgga
    15421 gttttcctag aaagttcaaa tagagtagaa aattttgaac attcaggata taaatcaaga
    15481 atatctttat ttggatttat atataaaata tcacacccaa gttgtgataa aaaatataag
    15541 aaaaaaagtt cttgcctttt aatatttcca aagtatacta tctttggtga aatagtagtc
    15601 ttcaccttgt tataaaataa tttaggtaga aaatagtcca tccaacacaa aagtttaact
    15661 gcaaagtttt tgatgataga ggatgtacaa tttggcatac ttgtggaaaa taaatctaaa
    15721 gtatttttaa atgcaatctt tttagtccaa tttaaagtgt cattttctat tttacatgga
    15781 aagtcgatat tatataaatc ccaactgtta aacttagaac tatcataaat attaaaaatt
    15841 ttaagcatct tatctgtttg ttctttgtca aaatttattt ttagattgtt taatctaata
    15901 tagggaattg taggattatt aaaaaaataa gtatctatat ttttaacttt ttctatgtat
    15961 ttatcaattg aatcatcaac acctatatat cttgtaaagt ggacttcttt aatatatgaa
    16021 gaactcttat ttttataggt agtatcagat ataaattcaa ttagaggttc agttgacttt
    16081 tttattgaaa tctttaatgg tttaatagta caaatcatat tttttctcct ataataagat
    16141 gatataatct attataaaac aacttaaaac aatatagtac aataattttg tagtttcaaa
    16201 ataaaatatg tacttgttta tataaatgat atataattac atattaaaca tagcttttat
    16261 acatttaata aagtatgtaa aatctatgta aaaagtaaga aatataaaca tttttttaat
    16321 tatattttaa aaatatcaag caagttttct tcacaaatga ataaaaaatg ttataatatg
    16381 attaaagaaa aagataaata attagcaaga tttgtttagc taaaattaac aagggagcga
    16441 taaattatgg gtataacatt atcaaaaggc caaaaggttg atttaactaa aggaaatcca
    16501 ggtcttaaaa atattttagt aggtttagga tgggatacta acaagtatga tggtggattt
    16561 gactttgatt tagatacagc agcattttta acaggagcaa gtggaagtgt tactaatgat
    16621 ggagattttg tattttacaa caatttaaaa catacatctg gagcagttga gcatctagga
    16681 gataacagaa ctggcgaagg tgatggagat gatgaacaaa tagttgttga tttatctaag
    16741 attcctggag aaataagcaa gatttctttt actgttacaa ttcatgatgc gacagaaaga
    16801 agacaaaatt ttggacaagt aagtaattct tatataagaa tagtagataa agacacaaat
    16861 gaagagttaa taaagtatga attaggagaa gattttagta tagaaacagc tatagtagta
    16921 gctgaaatat ataagcacaa tggagaatgg aagtttaatg cactaggttc tggttttgaa
    16981 ggtggtcttg ctgcattatg tggaaacttt ggaataaact tataatttca actataacaa
    17041 taaactaaag ggggatattt tccatggcta tagtattaaa aaaaggacaa aaaatcgatt
    17101 taactaaggg aaatccaggt cttaaaaata taaaacttgg actaggatgg gatacaaatt
    17161 catttgatag tggctatgat tatgatttag atgtaagtgt tttcatggtt ggagaatctc
    17221 aaagagttga aaaagatgaa gattttatat tctacaataa cttaagacat ccatctggag
    17281 cagttgaaca cttaggtgat aatagaactg gtgaaggtga tggagatgat gaggaaatat
    17341 tagtgaattt agagacaatg ccaaagcata tacaaaagat agctgtaaca gttacaatat
    17401 atgaggctgc tgaaagaaga caaaattttg gacaagtaag caactcatat ataagagtat
    17461 taaattctga aaatgatgaa gaaatattaa gatatgactt aggtgaagaa tttagtatag
    17521 aaactgcaat tacagtatgt gagatatata agtataatgg agaatggaag tttagtgcag
    17581 ttggagcagg atttgaaggt ggcttagaag cactttgtag aaactatggc ttaaatgttt
    17641 aatttaagca atataatata ttaaatataa ataacctata caaataatgt ataggttata
    17701 ttatataaaa ttttgtggag gtttagtatg tcgattaatt tatcaaaagg tgataaaata
    17761 gatttaaaaa aatctaaccc aggactgagt aacatacttg taggtctagg atgggaccct
    17821 gtgcaacaat ctggaggagg tttttttaaa tcgttatttg gtggaggaca agctgatata
    17881 gattgtgatg catctgtatt tatgcttaat caagaaggta aattaagtgg aataaaagat
    17941 ttaatttatt ttggaaatct aaaaagtgcc tgtaaatcag ttttgcatac aggagacaat
    18001 ctaactggtg agggtacagg tgatgatgaa caaatcctag ttaatttgga taaagttcca
    18061 tcaaatattc ataaattatt gtttgtagta aatatataca attgtgtaga tagaaaacaa
    18121 cactttggaa tgatagaaaa tgcctatata agagttgaag accaaggtaa taagaaagag
    18181 atagcaaaat acaatctatc tgataattat tctgaaaaaa ctactcttat agtaggagct
    18241 atatatagaa aagatggaag ttggcaattt aaagcaattg gtgaaggaac aaaagatgcg
    18301 ggtcttaaag aagtaatgca aaatttagac aggattgagt gtgcatatgg aatttaataa
    18361 tgatatcaaa tattataatg tagataaaga agaagttaca aaagacttat ctgttaatcc
    18421 tgaaaaaggt ttatcagaat cagaagtcaa gactagaaga gaaaaatatg gtttgaatga
    18481 atttactcca aaagaagagg gtagtttttg ggatgattta aaagaaagtt taagtgaacc
    18541 aatgatagtt atcttgatta ttgcagcagt agtaagtgca gtaattgggg aaactcatga
    18601 tgcaataggg attgtaggtg ctatagcaat aggtatagca ataggtatga ttacagaagg
    18661 tcgttctaag aaagcagcag aggctctttc taagttgact gaaaacatag aagtaaaagt
    18721 attgagagat ggggaagtac atcaaatttc taagagtgaa ttggtaccag gtgatatagt
    18781 atatattgaa actggtgaca tggtacctgc tgatggtaga cttatagaaa gtataaacct
    18841 taaaattaga gaagatatgt taacaggaga atctgatgac gtatctaaaa agtgtgatgt
    18901 agtagtttct atggaagaga tagaatctaa aggagcagta gtagtacagg agccaatacc
    18961 tgcaaaacag ataaatatgg tgtttggtgg tacacttgta gcttatggta gaggagctct
    19021 agttgtaact tcaattggag ataatagtga aatgggtaaa attgctcaaa acttatcaga
    19081 aacagatgag caaactccac ttcagataaa acttggaaat ttaggtggtt tgatagccaa
    19141 agtttctagt gcagtagcag gattactatt tatatttatg gttttccaaa tgataagtaa
    19201 gaatgtatta aatgtagata tgtctggaat attaccgttt ttagaatcaa tagacccagt
    19261 taaaacagca tttacagtat gtgttgcgtt gatagttgca gcagtgccag aaggtcttcc
    19321 aactatgata aatatgacac ttgcaataac aatgcaaaaa atggctaaaa taaatgcatt
    19381 ggttaccaaa aaagaagctt gtgaaacgat aggttctgtt tctgtaatat gttcagataa
    19441 aactggtaca ttaactgaaa atagaatgac tgtagaagtt gcatatgttg atggcagata
    19501 tattgaaagt tcagaagagg aaataaatag ttattttgaa gaaaactgta tgataaactc
    19561 tacagctgat gtagagcaca atgatggtga tattaagtat ttaggaagtg ctactgaatg
    19621 tgcattgctt ttatattaca aaaacgtaga ttatagacaa gttagaaaaa attctaatat
    19681 agtagctcaa aatcctttta cttcagattc aaagagaatg acttctgtta taggtcaaga
    19741 taatcatcat gtactattat caaaaggagc accagaagtc ttacttgaat tatgttcaca
    19801 tattcaaaga ggaaaagaca tagttccatt aactgaaaat ataaaacatg agattttaga
    19861 agaaataaag aaattgcaaa taaaatcaat gagaacttta gggtttgcat ataaagaaat
    19921 ctcacaagct gaagaagaag cagcagtaac tgctgaaagt gatgctatga atgtaagtgc
    19981 aatggaaaat aatttagtat ttagtggttt tgttggtata agagacccac ttagaaaaga
    20041 tgttatagag tctgtaaata cagcaaataa agctggtgtt tctgtaaaaa tgcttacagg
    20101 agataatata aatacggcaa gagctatagg tgaagagtta ggcctattaa aaaataatat
    20161 gagagtggtt gaagcttcat atatagatac tttagatgat gaagaattaa aacaagagat
    20221 acaaagtatt tcaatcgtgg caagaagtaa accagatagt aaaatgagaa tagtttcagc
    20281 tctgcaaaag agcggggatg ttgttgcagt aacaggtgat ggtataaatg atgctccagc
    20341 actttcaaaa gcagatgttg gtatagcaat gggtatatct ggtacagagg tatctaaaaa
    20401 cgcagcagac ataattttaa ctgatgatag ttttagtacc attgtaaaag gtattaaatg
    20461 gggtagagga atttatgata acttccaaag attcgttcaa ttccaattaa ctgtaaatgt
    20521 aattgcattt ttagtggcta taatatctca agttatggga caagagatgc catttactac
    20581 aatacaatta ttatgggtaa acataattat ggatggacca ccagcactag cattaggttt
    20641 agagccagtt agagaccatg tgttaaatag aaaacctgtt gatagaaaag ccaatataat
    20701 ttctaagtca atggtgtata caatagtatt aaatgcattt tatataacag caatactaat
    20761 gttacaatct acatttaatt tcttaggagt tgatgaagct cataagggaa cagtaatgtt
    20821 tgcgttattt gcttttagcg cactgtttaa tgcatttaac tgtagagagt ttaatgcaga
    20881 tagtatcttc cctaatttta cacataacaa attagcacta caaataatag tgttgacagg
    20941 aattgcacaa gtaatattta ctcagctatt ccaagatttc tttaactctg tagcaatgga
    21001 ttttatgaca tgggtaaaag ttttatgtgt agcagcaagt gtaattgtgg ttaatgaagt
    21061 tgtaaaatgt ataataagac tggtaaaacc aaaaaagact gcttaaaaca aattttttag
    21121 aagaaaacag actgactcaa aatatgaatt ttgggtcagt tctgaataaa catttaaatt
    21181 gggaggatag ttatgggtga ctcaaaggta tttgaaaaaa tattctccag ccaatcagat
    21241 agaggaaatt acactccttc aaagggatat ctaagttatt ttatttcgta tattggttta
    21301 gaagatgaag ttttgtacaa tctcgagata tttaagacaa aacaaaatat tgatagcaaa
    21361 aaagatattg ctttatttac tgatgttata gcaaatccta gtgattttga tattataaat
    21421 tattttaaaa gtggacttca aaaatataga actagcatgg aagatgtaga tataaatatt
    21481 ttgggatttg aagagattga ttataaaata aagcaagcaa tggatagagt gttaaaagaa
    21541 gaggaaaaag aatttactaa tgatagagtt aaacaaaatt ttatagtgaa aataatggca
    21601 tggataaaaa tttatatcgg tgctttagat ataaataaaa atgaagctcc aaaagtaata
    21661 ttttatggag atataaaaaa acatgaagtt tatttactct taatattata cttggcaggt
    21721 tttgatgttt tatatcttaa tccaaattca aagagtaata tagatatttt aaaatcagaa
    21781 agatacaata tagagtttga agaagccaac ataatagaag aaaaaatttc ttttgaagaa
    21841 agggttattt taggggaaaa aatagataaa agttctgtaa aaaaagcttt tactgttggg
    21901 gcagaagcat caaagcgtat aagtgaggaa ttgttaaatg atgcaggatt tataaaacct
    21961 tggcaactcc aagatagaaa aataaaaaat ctacttctaa gttcaactgt tgatgaaata
    22021 tcaatatact ggaatcaacc acttaaatta agaccaggat ttaagtttaa tgatgctatt
    22081 gtagaagctc caaattttct ttctaagatt aatggaatat ataatgataa gaatgaatat
    22141 attaagtttt tagatttatt aagagattcg gaaagttcaa catttattga atttaatgga
    22201 gatgtagata gattttcaaa agcttttacg agagaagctt ttagtttatc ctttttattg
    22261 gattcaaaag gtgtaataga taaaaactct gttttaaata ataaagatta ttccatatca
    22321 acactagcac ttaatcaaca aattatgatt ttagagaaag tggaagagtt gttagaagga
    22381 agtatgtttt tgaatggttt aagtggagaa gacaagataa aaggtctatt tacagtactt
    22441 cacatggata agaaatttgt tcatatgatg aacaatttcg attactcact tataaaccca
    22501 aaattgatta tatacatgta caagagtatt gtatttgata aagaaatagt ttttcttatg
    22561 cttttactta gtaagattgg atttgatatt atcatattat gtccaggagg agaaaacaat
    22621 atagagaatg ttataaataa ccaattgatt gatatacaca gattggataa aatggtatat
    22681 gaccttaaat taaattcgct tgaaaatgat atacctttac ttaaaaaaat ttttggaaag
    22741 aggagacgat tttagatggg gataaatata aatagttttt caaaagataa gaactctgta
    22801 gaggttactg agaatttgcc tacagcagtt caagaagaaa acttcgatat aatggaatat
    22861 acaaataata aaaaaaatga actgagaaag tctaaggaag ttgaggcatt gacatccctt
    22921 atagaagtag aaaatccaga tacgatttta cagtttggta ggaaagcatc tgaaggagta
    22981 gcaagagttt ctgatagcct tttaaataca ataaaattaa atagaaatga agaaaattct
    23041 aaaatgctgg ttcatttaac taaaataatg gataagtttg atttagacga ttttcaagaa
    23101 actaaggaac caaatttcgt tcaaaaactt ttcaaaaaag ctaataatgc cattgaaatg
    23161 atgtttcaaa agtatgaaac attgggagga gaagttgaga aaattcaaat tgagcttgag
    23221 caatacgaaa gagatatagc actttctaat aagcaaatag gtgctatgtt aaatgaaaac
    23281 tttgagtttt ataatgaact tcaaaaatat attgttgctg gagaaatggc aatagaagaa
    23341 atggataatg aaatactacc tttctttaaa caaaaatcag agactagtgg agaccaaatg
    23401 gatgttgtca attatcaaga actattaaaa gtttatgata tgttaaatca aagagtctat
    23461 gatttaagaa tagcagaaaa tatagctatc caaacaatac ctatgcttag aggtatgcaa
    23521 cataataatt atggtttaat tagaaaaata aatagtgcat ttgtcgtaac tttaccagta
    23581 tttaaacaat gtttatctca agctatatta cttaaaaagc aagaattaca agcaaaatct
    23641 ctaaaagcac ttgatgacaa aactaatgaa ttattgctta gaaatgcaca aaatgtaagt
    23701 actcaaagtg cacaaatagc tagaatggct ggaacaagtt ctgttcaaat agaaacattg
    23761 gaaaaaatgt acaatactat caagtctggt atagatgaaa ctatgagaat agaagaaaac
    23821 aatagagctt taattaagga taatacaaag agattggaag aattaaatac gactataatt
    23881 tataataagt agtattatat taagggctaa tttgtataaa gttggccctt tctatgtata
    23941 tgaggaggct agacattgaa atatttcgat tatatctgca aagatgattt ggagagaatt
    24001 tttttgaagg agccagaaga ctttagtgct aagactgaaa aagatgtgtt gaaatatgca
    24061 ctgggcgctt ttttatacgt tccagctact caatataata tgatttataa gagtattata
    24121 ggggatgtta agggagttag accattagct atttgtttag aagatgctgt tggagtaaat
    24181 ggagagttag aggctataga aaatcttaga ttaattttaa aaaatattag caatgaaagt
    24241 ataactaata aagatggaat accactaatt tttgtaagaa taaaagatgt ggagcaactg
    24301 ttgagaatta aagagattat aattaaaaat agtcattcta taacaggaat attaattcct
    24361 aaagcaaatt cggagttgat tgaaaattgt atagaagctt tatattctat gaatcttcaa
    24421 gatatgtatg ttattccaat aatagagaca agagaattta tatataatga aaaaaaagaa
    24481 cttagtttta ctaatttgta caatgctatt ttaagacata agtcaagaat attaagcatt
    24541 agagtaggca taactgatat attagggatg tatggtataa ggagagataa aaacttctct
    24601 atatataata atttgatttg ttcttcattt atattagata taatcacata tcttcaaaga
    24661 ccagagttag atatacctat aagtggtgga gtatcagaat tttttgatat gacaaataag
    24721 gatataagaa ataagtatat agaagaaata ttattggata aatatcatgg attaattgga
    24781 aagaccgtaa ttcatccaat gcaaattcaa attgtacaag cgctatctac agtatcatat
    24841 gaagatttta ctgatgcttt ggatatactg gatagtacag acagtaagta tggtgtgagc
    24901 aagggtgtat taggagagag aatgaatgaa acaaatccac attttttatg ggctaaaaaa
    24961 acattgattc tatctaaaat atatggtgta ttaaataaag gagtagacta tgaagaattg
    25021 cttaaatttt aattgtgaga ttgacattat tgataatcca ttgaatttag aactaaatga
    25081 ttttttagac gtaggagtaa gaaataatag caaaagacgt tttttgttta tctcaaagaa
    25141 actcggtaaa catttagcat gtaagccaag tgagatggat aatttgggaa aattaatggc
    25201 tacaatttat aatgaaaaaa ataaggaaga atcaaatggt actgttatag cttttgctga
    25261 aactggtaca gctgttgggc atagtttttt tgattactta tgtggagatt atgaatttat
    25321 tcatactact agagagcagt ttgacgagtt agaaaatctg gaatttttag aggaacattc
    25381 ccatgcaaca gaccagaatt tatactttgg tatgcttaac aactttaaat ttggagatga
    25441 gataatattg gttgatgatg agattaccac agccaataca tgcatgaata taattaggaa
    25501 aattcaaagc atttatccta aaaagaaata cactatatgc tcaatactca attgggtaag
    25561 tgatgaaaat ttaagtaaag ttactcgttt agaaagagag ctaaattgta agatagattt
    25621 tgtatactta tttaaaggaa actttaaatt ttatatagat gaagatatta tttcaaatta
    25681 caacgataat tatagtatta ctaaattaga gactatttca agtgataaag taaatttagt
    25741 agtaaatcat atttatgttg atttagaaga ttatattgga aataagaaat atgtaaaata
    25801 tacaggaaga tttggaatta atagaagaga acaatttaga ttaaaagaaa tcgttaagag
    25861 agaaagtaaa aaaataacta tggccagtaa agaatcttta aaaaatgaaa aagaaaagat
    25921 tctattttta ggtactgaag aatttatgta tatacctatg ctgtttgcaa aagaaaaaga
    25981 aaatcaatat gatatatact atcattcaac aacaagaagt ccaattatta atattgataa
    26041 gaagcattat cctattaaaa gtaaatttat gctaaagagt ttttataata tagatattca
    26101 aaattatatt tacaatatag ataaacataa tttttctaag tgttatttat ttgtagagct
    26161 gaataaaagt gaaaattctt atctagaatt tattgatata attaaaaaaa caagtataaa
    26221 agaattaact atagtatgtt gtagctaaag tgttttaaca ggataaaata tatcacgatt
    26281 ttaagatagg agttgagtca tatgagagat tttagcacgt attctaaaga tgatgttatt
    26341 tttttattaa aagatatatc aaatttaatt gaagaagaag gaaataaaga aagagaatta
    26401 aaaatacaaa atggtagaca ttattcagaa atgataccaa tagagtatga agttagtgaa
    26461 gattatctga atttatatca tgagaaacta agagaaaata aagataagct tgcattttct
    26521 ataggagtta tgtgtgaaaa aataattaaa aaaaatggaa atgatgtagt actagtttca
    26581 cttgctagag caggaacacc tattggtatt ttggcaaaaa gatatataag aagcaagtat
    26641 aatgtaagtt tacctcacta tacaatctct ataataagag ataagggaat agatatgaat
    26701 gcaattgaat atatagtaaa gaatcatccg agctcaaaaa ttcaattttt agatggatgg
    26761 actggtaagg gaactatatc aaaagaactt gaaaaagcat gtgatgaact taatgttaaa
    26821 tttaaaaaac attttgactc aacacttgct gtattagctg accctgctgg atattcagga
    26881 ctttatggaa ttagagaaga tttcttaata ccaagtgcat gtttaaattc aaccgtttct
    26941 ggtttagtaa gtagaactgt actaagagag gatttaattg gtaaaaatga ctttcatgga
    27001 gctaagtttt atagacattt aaaagataaa gatgaatcta tgaactatat cgaaactata
    27061 gaagaatgtt ttaaaaatca atttaagaat atatctgatg aagttgaaaa ctgggaaagt
    27121 gacattataa caagagatgg atactttgat gttttaaata taaaagaaaa gtataacata
    27181 acagatatta actttatcaa accaggtgta ggggaaacga caagggtttt acttagaaga
    27241 gttccgtata agattcttgt taaagactta aatgataaat ctttagacca tatattcata
    27301 ttagctaagg agaaaaatgt tgaagttgaa caaatggatt taaaagccta taaatgttgt
    27361 ggaataataa aaaatatgaa agatatttag gaaatatatt agacaggaga atattgaatg
    27421 attgtttttt cagatttaga caggagtatc atttactcca ataaattttt aaatgctgat
    27481 agtaaatatg ctaatataga aatttatagg gaaaaagaaa tatcttatat atctttagat
    27541 actataaatt taataaagca aatacaatat tatggtatgt ttatacctac aactactaga
    27601 acagtggagc aatttaagcg tatagaattt aataaatatg ggatttattt tccttggagt
    27661 ataacttcaa atggaggagt tatacttaaa gataatgaaa tattgaaatc atggagtgaa
    27721 aaaatagata aactaaaacc tgattatgag cccattgaaa gtatgattca taaatttaaa
    27781 gactatttga atgtagatgg aataactaat tttaaagttg cagaagatac atttttttat
    27841 atagttgttg atttctcaag atttaatttg gattcaatca aagaatatac aaatatattg
    27901 gaatcaaaaa attggaagtt ttatgtgagt ggcagaaaaa tatattttat accaaaagaa
    27961 atttcaaaag aaaatgctat aaaatacttg actaaagaat taggaataga atatttttat
    28021 gcagttggag attctattat ggactatggt atgcttaata tctcaaataa atcctatgta
    28081 ttgaagcatg gtgatataaa taaaaatgaa atagaaaatt catttatatc aagttctttc
    28141 aaaggaatga gtggaactga agagatatta tctaacattt tagacgagaa ctgcctactt
    28201 aatatataat gactgatata taaataataa actagaaaaa tctgaaataa attatagtaa
    28261 agtttatcat actaatttat ttcagatttt tatcaattta tatttaagta agaactcaat
    28321 atgttaaaat gaaggtggag tcattgccca gactgagata catacctcat catcataatt
    28381 ttcatacaca tgtggtatag tactgttaaa ataaaaacta tctccttcct ccaatagata
    28441 ttccttattt ccaagtgtta tcttcatttt accttttatt atatagccac attcttctcc
    28501 ttcgtgtgtc acaaattcac tgtttttttg tgtatgacca tctagcgtaa caagtataaa
    28561 ttctattttt ttcccagata aattaggaac aagaagttca taaaattttg tagaatcatt
    28621 agttactatt ttttttcgat tatttttctt tactactata tcagagtcct ctcttccaat
    28681 ctcttcaaaa aaataaccta tatttatatc aagagctttt gatattttcc acattgctac
    28741 aatagatgga acaaccatat tacgctcaat ttgacttatt aagcctgtac ttaaatctga
    28801 attttttgct aaagtagaga tacttatatt tttagatttt ctaagagatc taattttgct
    28861 tccgacatct atattcatta gatacaaacc ccctcagaca tgattataac atataattta
    28921 taaagattaa taaaataaaa aaaagtttta taatgcatgt attttttatg ttatattgaa
    28981 ttttctgaaa agatgcatta taatagtatt aatattcaat atattgaata ttgataatga
    29041 tattttaatt taatgaaatg gaggtaatta agatgaagag ttttgtaagt cctgataata
    29101 tttgggtatt atgggcagtt gtaactggat gggcagcatt tagtatttac ttggaacaaa
    29161 agtacaattg ggcatcaaaa gtgtcgggag caataatagc tcttgtagga gcaatgttat
    29221 tatcaaatct aaatattata ccagtagagt ctgttgtata tgaccaagta tggggatatg
    29281 ttgttccttt agccatagct ttattgttat atcaatgtaa tattaaaaag atatggaaag
    29341 aaagtggaag attgctcatt atatttcttg taggttctgt aggaactgta cttggagcta
    29401 tgattggatt tttagcatta aaaaatgtag taccagattt aaatattgtt gcagctatga
    29461 tgacaggttc ttatataggt ggaaatgtaa actttgctgc tatgtcaggg gcgtttgatg
    29521 cgccaggaga attagtatca gcaacagttg tagcagataa tcttttaatg gcattatatt
    29581 tctttgtctt aatagctata ccatcaattg gattttttag aaaacatttt aaacatccac
    29641 atgtagatga aatggaaagt ataggtataa ctgaaggaga aacagtagca gcaaattatt
    29701 ggggaagaaa agaaatatct ttaaaagata tagcaatagc cattggtagc gcctttataa
    29761 ttgtcgcagt atctgtagaa ctatcaactt ggtttaaagc tataatacca ttatcaaatc
    29821 cttttttagc aatgttaaat actttatttg gaaatcaata tctaataata actactttga
    29881 ctatgttgtt tgccacattt atgcctaatt tctttggtga tataaaagga gcacaggaat
    29941 tgggtacatt tttaatatat atattctttg ttgtagtagg tgttcctgct tcaataacat
    30001 taataattca aaaatcacca ttactacttt tatactgcgg aataatggta ctaataaaca
    30061 tgctagttac atttatagtt gcaaaaatat ttaagtttag tttagaagaa gctattcttg
    30121 catctaatgc aaatattggt ggtcctacaa cagcagcagc tatggcaata tcaaaaggat
    30181 ggtctaagct agtagggcca atacttatag ttggaacatt tggatacata gttggtaatt
    30241 attttggtct tttagtagga aatattttga tataatttta cgtgaaaaga agttgttttt
    30301 tagaaagatt tttatttaat tggaggttgt tatgattttc gatggacatt cagatacttg
    30361 ggcagatgta acaataagaa ggcttaacgg ggaaaaagat gtatttagaa gaatccatct
    30421 tgataatttt aaaagagcaa atatagaagg agctattttt gtaatatgga tagacccacc
    30481 atatgataaa gagcctgaaa aaagagttaa acaaattata agctctataa aagatgaaat
    30541 tgaagatatg aaagatgttg taaacatagt aaaaaaatat tcagatatag ataaaggtat
    30601 aagtgaaaac aaaataaata ttttaattgg tcttgaagga ttaagccata ttggaagaaa
    30661 tatagattta ctagattatt tttataaaga agttggtgca agacatgcat cattaacttg
    30721 gaatgaacag aatgaacttg ctacaggatg tttaggggat gaaaatcgtg gtttaactga
    30781 aataggtaaa aaagcagtca ataaaatgga aaaattgggc atgatagtag atgtgtcaca
    30841 tttaaatgaa aaatcatttt gggatttaat gtctgttgca agtaagccag ttatagcttc
    30901 tcattctaat gctaggagtt tatgtgatgc taaaagaaat cttacagata atcaattaaa
    30961 agaaatttca aatacaggtg gattagttgg aataaatagt tttagaggat ttactaatag
    31021 taatcctaag aagcaagatt taatacattt ggttgaccat atagactata tggtaaatct
    31081 tataggtata gaacatgtat cttttggttt tgattttagt gattacttag ggaaagagac
    31141 tataggttct tttactggaa atgctagtgt gccaagtgta gatggtcttg aaagcacaca
    31201 tacagcacag aacatagtag tagagcttga aaatagaggt tataaaaaga aagatataga
    31261 gttaatctct agtggtaatt tttataggct ctttaaagat atactaaaat aatataataa
    31321 agaacttaaa tttataaaat agtatattta tacaataata tctacatatt taaattggcg
    31381 ttcataattt attaaattaa attttaatgt tactagaagt ttcaattcta gtaacatttt
    31441 ttatgttaaa aatagatata gatatatatt gacaatgata attattattg ataaactata
    31501 aaagtgaaaa tgataataat tttcatttta attaataaaa gatttttaag gagggagaat
    31561 gaagaaaagg tatttagtca taggaataat aattttgtct tatttttctt tatttatagg
    31621 agctgaagat ataaatataa tgcatatatt tgcaaaaaat cagcataagc ttatgatatt
    31681 tatcatgagt agggtaccaa gattaataag tatattgatt gctggtgtag gaatgagcat
    31741 agcaggtctt ataatgcaac agataagtaa gaataaattt gtatcaccta caacaggtgc
    31801 tacaatagat gcagctcaat ttgggatagt aatatgtatg cttttagtac caactgcctc
    31861 tatttttaca aagacaataa tagcatttgt attttctctt gtagggactt ttacgtttat
    31921 gaaaataata gggaagttac aatttaaaaa tataatcttt gtaccattag taggtataat
    31981 gtttggaaat ataataggtt ctatgacgga ttttatcgcg tataaatacg atttgagtca
    32041 aaatgttagt tcttggatgc aaggtgactt ttctatgata cttaaaggaa attatgagat
    32101 attatatatc acaatacccc ttataatatt agcatatatt tatgcaaata aatttactgt
    32161 tgttggtatg ggtatggatt ttgcgacaaa cttaggtctt agttacaaaa gaattgtcaa
    32221 tataggtctg ataatagtag cacttgttac agtatgtgta gtcgttacag ctggtaatat
    32281 cccttttata ggtctaatag ttcccaatat cgtatccttg tatatgggtg ataatataag
    32341 agcaagcata tggtatactg gtttacttgg agcggtattt gtacttatat gtgatatttt
    32401 tggaagaatt attatttatc catatgaaat atctatagga cttacagttg gagttatagg
    32461 aagtatacta ttcctttatt taatattaag gaggaatgtg aatgaagctt agtctcaatc
    32521 aaaaaattta ctttgtaagt atattactat tcatatcaat agcacttttt ataatttatg
    32581 gaatagacat gaatcattta gaatatgcat tatcacaaag aataccaaaa atatttgcta
    32641 tggtattagg tggaggatgt atagcattta ctactgtagt gtttcaaaca attacaaaca
    32701 atcaaatatt aacaccaagt gtactaggtt tagattcatt gtatgtaatg attcaaacta
    32761 taatagtatt catatttggt tcaagcagta gtctgattat aaatgaaaat tataatttta
    32821 ttattaatgt agctatgatg attggagctt cattgttatt atataagatg ttatttgaaa
    32881 aaaataaaaa taatattttc tttttaatcc tagtaggtat gatatttgga accttattta
    32941 aaagtgcaac aacatttatt caagtaatga tagacccgaa tgaattttta gctttacaaa
    33001 ctagtataat ggcaagttta aacaatataa acactaatgt attgttaata gcatttataa
    33061 taattattgc cataatacct tttatatatg atgaaataaa gtatttagat gtattgtcgt
    33121 taggtaaaga acaagcaatt aatttaggtg tagattttga taaggcagtt aaaaaaatga
    33181 taatattaat agcaatttta gtctcaatat caacagcact tattggacct atgacatttt
    33241 taggactttt acttgctaat ataacaagag aaatttttaa aacatataag catacatact
    33301 tgatttcggg ttctatgttg atagggatga ttacattgat aataggtcaa ttttttatac
    33361 aacatgtatt taagtttgat acgactttaa gtgtagtaat aaattttata ggaggaattt
    33421 actttattca attactatta aagggggcaa atagatgata gagataaaaa atatatttaa
    33481 aagatataag aataaaaatg ttgtagatga tgtctctttt agtatagaaa agggaaagat
    33541 aacatctttt ataggaccaa atggagctgg aaaaagtaca gtactatcaa tagttacaag
    33601 gcttatagga ggtgatggcg gagaagtaat aatagaagga aaaagcttga ctaattatag
    33661 caataaagaa ttagcaaaaa aaatagctat tcttaaacaa tctaacaata taactttaaa
    33721 gcttacaata agagaattag ttggatttgg aagatttcca tatagtgaag gtaatctaac
    33781 taaagaggat gaaaattata ttgatgaagc aatagaatat atgaagttga ctgatataca
    33841 acataagtat ttagatgaat tgagtggagg tcaaagacaa agagcttata tagcaatggt
    33901 aatagctcaa gatacagagt acatattact tgatgaacca ctgaacaatt tggatatgaa
    33961 ccattccgta cagatgatga aggtacttag aagtctttgt gatgagttag ataagactat
    34021 agtattagta atgcatgata taaattttgc atcatgttat tcagataata tagttgcatt
    34081 aaaaaatgga aaagttgaaa aagtaggtcg tacagatgag atagtaaatg aaaaagtttt
    34141 agaagatatt tatgagatga attttaacat taagaatata aatggaaatc gaatctgtat
    34201 ttatttttaa aatattgtat atgaaaataa gcttgctata tgatttttta taaagttttt
    34261 aaattaaaat aaagattaat aggagagagg aaaatattga tatgaataaa aaagcagcaa
    34321 tagtagcagc agtagctatt ataggattag tgacagtgtt tgcattaggt ggaagtaaaa
    34381 agaatgaatc aaaaacatca gaaaattcaa ataataccat aaagataact cataacttgg
    34441 gagaaacaga tgtaaaatta aatcctaaaa aagtagtagt atttgattat tcggctttag
    34501 atacaatgga tgcattaggt gtagctgaga atttagtagg acttccaaaa acgagtttac
    34561 cagcatcttt agaaaaatat aaggataaga agtatgcaga tttaggaggt ttaaaagaac
    34621 cagatttaga aggtataaag tcggcaaatc cagatttaat tataataaat ggaagacaag
    34681 aagattttta tgaacaattg tcaaaaatag caccaacaat aagtacaagt aaagatgata
    34741 agaagtactt agaatctgtt aaaaataata tagacaaaat agcaaaaata tttggagtag
    34801 aagaaaaagc aaatcaagaa tttagtaaaa ttgaaaagaa aatagaaact ttgaataaaa
    34861 aagtaacaga taaaaattta aatgctttaa ctataatggt aaatgaaggt aatttaagtg
    34921 tatttggtga agaatcaaga tttagtatat tatacaatag ttttggattt gaaaataaag
    34981 ataaaaatat aaaagaatca agtcatggac aaaatattac ttttgaatat atagctaaac
    35041 aaaatccaga agttatgttc gttatagata gaggaatagc tactggtagt gatgtaaaag
    35101 aaagctcaac tgcaaaatct gttttgaaca atgatataat aaaatctatg gatgcatata
    35161 agaatgataa tataatatat ttagattcac caacatggta tgtaaatgat ggtggtctaa
    35221 catctttaaa taaaatgata gatgatgctt caaaagcggt aaattaataa tccgatataa
    35281 tcaaaatgta ttttttgtaa aatgattatc aaataaacta ggagttttta agaactccta
    35341 gtttattttt tgcataataa agatatagtt taaacaataa tattatttat aataagataa
    35401 atctattc
//
LOCUS       DAMJWP010000469        17198 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.guided.061, whole genome shotgun sequence.
ACCESSION   DAMJWP010000469 DAMJWP010000000
VERSION     DAMJWP010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17198
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.guided.061"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(241..765)
                     /locus_tag="PC700_003782"
     CDS             complement(241..765)
                     /locus_tag="PC700_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2582988.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQIMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(830..1582)
                     /locus_tag="PC700_003783"
     CDS             complement(830..1582)
                     /locus_tag="PC700_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDF2582989.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDISKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(1613..2911)
                     /locus_tag="PC700_003784"
     CDS             complement(1613..2911)
                     /locus_tag="PC700_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HDF2582990.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVESTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEDIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMEDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQQGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREVNVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(2928..5192)
                     /locus_tag="PC700_003785"
     CDS             complement(2928..5192)
                     /locus_tag="PC700_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HDF2582991.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(5447..6265)
                     /locus_tag="PC700_003786"
     CDS             complement(5447..6265)
                     /locus_tag="PC700_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDF2582992.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGIDEKIIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIIL
                     LDEPTAGLDPVSTRSIVDIIKGLNKDNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFVNENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(6258..6956)
                     /gene="cbiQ"
                     /locus_tag="PC700_003787"
     CDS             complement(6258..6956)
                     /gene="cbiQ"
                     /locus_tag="PC700_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HDF2582993.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIKNIFVLSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEIFSFN
                     VGISKASIDMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKMHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(6959..7243)
                     /locus_tag="PC700_003788"
     CDS             complement(6959..7243)
                     /locus_tag="PC700_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2582994.1"
                     /translation="MSAKTKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEI
                     TKINPDYEPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGVQKGKRS"
     gene            complement(7233..7985)
                     /locus_tag="PC700_003789"
     CDS             complement(7233..7985)
                     /locus_tag="PC700_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HDF2582995.1"
                     /translation="MKQNIKLGVIAALMLIVLTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLVGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIISFLIFKALKKKDG
                     NNAMPVFLAAAIGDLATYTVTSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(8174..8366)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(8498..10270)
                     /locus_tag="PC700_003790"
     CDS             complement(8498..10270)
                     /locus_tag="PC700_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HDF2582996.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDIILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYNMDKHPALDSMTRRIVNDIRNIDTN
                     QDMMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(10284..11213)
                     /locus_tag="PC700_003791"
     CDS             complement(10284..11213)
                     /locus_tag="PC700_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2582997.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            11442..11630
                     /locus_tag="PC700_003792"
     CDS             11442..11630
                     /locus_tag="PC700_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2582998.1"
                     /translation="MEYMSCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKN
                     AEKPVDGRAKRRKEVQYE"
     gene            11623..12324
                     /locus_tag="PC700_003793"
     CDS             11623..12324
                     /locus_tag="PC700_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2582999.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHCGAEGLHLLDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDCIIINGMIIDKTNRIVSINNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            12326..13249
                     /locus_tag="PC700_003794"
     CDS             12326..13249
                     /locus_tag="PC700_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2583000.1"
                     /translation="MNTSVYLLLILALSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            13406..14329
                     /locus_tag="PC700_003795"
     CDS             13406..14329
                     /locus_tag="PC700_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2583001.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHVKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGCEKRVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSKYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDHTLRLYNTTLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            14322..15059
                     /locus_tag="PC700_003796"
     CDS             14322..15059
                     /locus_tag="PC700_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2583002.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPTKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFASIALGRRK"
     gene            15070..15843
                     /locus_tag="PC700_003797"
     CDS             15070..15843
                     /locus_tag="PC700_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2583003.1"
                     /translation="MWKLLWAECQKIRRSKIVWITFFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETKLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIISLLLCACLSAFILKGLKHNEKN"
     gene            15859..16011
                     /locus_tag="PC700_003798"
     CDS             15859..16011
                     /locus_tag="PC700_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2583004.1"
                     /translation="MKKIKKLIILIVFLFLFGMLHCYTEWKFNNDPPKGVIERINEWT
                     DKIGLD"
     gene            complement(16384..16992)
                     /locus_tag="PC700_003799"
     CDS             complement(16384..16992)
                     /locus_tag="PC700_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDF2583005.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
BASE COUNT         5541 a         2991 c         2158 g         6508 t
ORIGIN      
        1 atatttattt aatacattat tatttaaaaa taaatatata aacaatcata ataaaatttt
       61 tttaataaaa ctttattttt tagttaaaat catacaatat atagctttcc acaatacaaa
      121 taaattaaaa agtgaaatgt atttgtctaa aaacataata acatttcact ttatcctcat
      181 ttttaagtaa tatatatttt taatttttat ttataactca atctagtttt tatataagtt
      241 ttagtttttt tctacatctg agtaatctat tccaaaagtt tctacagtaa cagacttcat
      301 aatttgtgga gtttgtggct tatcagctga atctgttcta actgatgcta tttcatcaac
      361 tgtatccata ccttctatca ctctaccaaa acctgcatac tgaccatcta agtgtggtga
      421 gtttttatgc attatgaaaa attgagaacc agctgaatta ggtgccatag ttctagccat
      481 tgatatgact cctctttcat gttttaaatt gttagtaaat ccattaccac taaattcacc
      541 ttttattgag tgacctgggc cacccattcc tgtaccattt gggcatcctc cttgtatcat
      601 aaatcctggt attactctat gaaatattat tccattgtaa tatccttcat ttactaatga
      661 tacaaagttt ttaactgtat ttggagctat atttggatat aactcaattt tgatttcttt
      721 tccattttcc atttctatag ttactatagg atttttattt tccataattt attaccttcc
      781 ttttcattag ttaaattaca taattacatt tattatatta tactctaaat taaagtctat
      841 caagtatatt ttttcctatt tcgtatcctt cttgataaat agttttacaa aactcatcat
      901 caaagttaag cgtatcttta ggagcaaatt gagtattagg tctaactact attattttat
      961 cttcatcata tactttttta atatcctctg gcaactcata attatatttt gtggatatta
     1021 aaataatttt atccacatta tccccaatta atggttcaat taatgcactc ctaattaaac
     1081 ctccatctaa tttgaaacca tcataaattc cttcttgcac atctttcaca aattgttctt
     1141 taaatccaag agttgcgttt ggatttgatg gtaacaaccc actgtatgaa atgctgataa
     1201 tggcttcatt acgaggcatt tttgatatgt caacaatctt ttctgatact actttattat
     1261 caacttcaac ataattaaca taaaagttca tttcttctat attactatca tctagttccc
     1321 ttaggttgtc tatagcagga gaattatcca cagtattatt tactattttt acatttccat
     1381 tctggatatc ttttatattt gtccacattt tctccaagtt atcaacattc cctgtgtaca
     1441 aataatatcc atttattgct cctattgatg tccctgaaat cgaataaaat ttatttattc
     1501 ctccatcata caaggcttta actactcctg cttgataagc cccttttgct cctccacctt
     1561 ctaaacataa tcctaatttc atagtctcat ctccaaattg tttatcttat atttatattt
     1621 caaaaaactt actaaatgtt ttatatatac tatagtggtc ctcaacattt cctaattctc
     1681 ttatagagtg cattgctaaa attggacttc ccacatccac agatggtata tctatatgag
     1741 tagaagatat tggacctata gtacttcctc ctctttcatc tgacctatta acaaattctt
     1801 gatactcaac attaacttct ctacatatat ttttataaat agctatagaa tatccatcac
     1861 tagtatatgc ctgactagca tttatcttta taactggacc ttttcccatt attggtttat
     1921 tagttgggtc atgcttttct ggaatgtttg gatgaatagc atgagctaaa tctgatgata
     1981 tcataaaaga tgaataaata gactcaaaga atccctctct atccttaccc attgatatgc
     2041 aaattctctc caatatattt aacaacatgt ttgaatctgc accttgttta gtagagctac
     2101 caacttcttc attgtcaaat acagccatta catttacacc ttgttgacct ttagtattaa
     2161 caagagcatt caaactagca tgtaccattg caagattatc ttgtctccca atagatataa
     2221 attcatcatt agcccctatt aaacttcctt tttcaaattc atataaaaat aaatcaaaat
     2281 ctataatatc ttccatattt acatctaagt tagatgctat ttgctttata agaaaattat
     2341 ctttttctaa tgtatcattt aacatcccaa ctaaaggtaa catatctttt tgcttattat
     2401 atgaatgacc atcatttata cttctattta aatgtattgc tagatttggt attatacaaa
     2461 ttggcttttt aaagtctata agcctttcat ttggttttaa tatactatca ccttttaaaa
     2521 ctactctacc agctatccca agaggtctat ccaaccatgt acttaatatt gctcctccat
     2581 agcattctgt atttaacttc aaataagatt tttcagcttc tatttctgca tttggtttaa
     2641 tactaaatgt aggagaatct gaatgagagc ctattattct aaatccatct ctttctatat
     2701 cttctgaatt tactacaaaa gctactatag ctgatagatt cttagtaaca taatactttc
     2761 caccaactct tagattccat ctttgattca aaactaattc ttcaaatcca tttttatcta
     2821 agatttcctt agtactctcg actgcatgaa aagacgacgg actatcatat atgaagtcta
     2881 ttaagttttt agcaaagttt atactatcca ttcctcattt acctccatta taaaatattt
     2941 agtgttgtat attctttatt tattttttta attccctgtt ggtcaaaagc tatagtaaca
     3001 tctgtaccaa ttatagaaac cactgtccca accccaaatt taggatgatg taccttagaa
     3061 ccaagtttta tatcatctgg attagtttct ttattagaat cttttattgt agcattaact
     3121 ttatctgcaa ctttagattt gcttatagta ctcatatact ttttagtata tttatctaac
     3181 atattataac ttgctttaga atagcttagt tcttttttta ctttgtacaa tctttctata
     3241 cattcttctg gcagttcttc cataaatctt gaagctattg caacatttgt tttaccatat
     3301 aatgttctct tctctgtcaa tgacatatat agctcttctt tagctctagt tataccaaca
     3361 taacaaagtc ttctctcttc ttctatttgt gaatcactca tagattttat agctcttgat
     3421 ataggaaata acccttcttc catacctgtt aaaaacacaa cagggaactc taaacctttg
     3481 gatgtatgaa ttgtcataag cgaaacccta tcattgtctt cttcatcatt tgattcagat
     3541 gtaagtgcta tacttgttag gaatgtctct aagtctttat tttcataagt atcatcatta
     3601 ctactttcaa actccaaagc tatagaaata aattctctca aattatcaat tctgtcttga
     3661 gcttcttcac ctttgcctgt taaatctttt tcatttttat ttcttatctc tacaagctcg
     3721 tccatatatc ctgtagtatc caagactttt tctataagtt tgctaacagg ataaacttct
     3781 tttatagttc taagagtccc tataatatca acaaatccac ttatacttgc ccttgcctta
     3841 gtagatatat ctgaatttgt ttctatatca attaatactg agtatatact ttcctgtttt
     3901 aagttagctc tatcttcaat tttttctata gttctaagcc ctatacctct tctaggaaca
     3961 tttataatcc tttttataga tatatcatct tgtggatttt gaataactct taaatacgcc
     4021 aacaaatcct ttatttcttt tctctcgtag aattttgtac caccatatat attgtatgga
     4081 atttgacttc tatttaaagc gtcctctaca ggtcttgcct gtgcatttgc tctataaagt
     4141 actgcaaaat ccttataagg tctattttct tttctagcta tttgtgcaat tgaatcagca
     4201 atgaaatcag cctcttctat ttcgctacca gttaattgta tctttataag ttctccttct
     4261 tttttttcac tccaaagctt ttttcttttt ctttctatat tattagatat aactttatta
     4321 gctgcatcta atatcacttg tgtagaccta taattttgtt ctaactttac aacatgtaca
     4381 ttgtcataat ctttctcaaa ttcaagtatg tttcttatat ctgcgcctct ccatccataa
     4441 atactttggt catcatctcc aacaacacaa atattttgat gttctcttgc taataatttt
     4501 atcaattcat attgagcttt acttgtatct tggtactcat ctaccattat gtatctaaat
     4561 ctacttctgt aatatgctaa tacttcatca ttagccttga aaagttctac agttttaagt
     4621 attaaatcat caaagtcaag tgcactattt ctttttaaac ggtcttgata taacgcataa
     4681 atatctgcaa tcttaaccat tctattatct gccatgttga tatctttaaa ctgctttgga
     4741 gtataaagtt tgtcttttgc tcctgaaatt gcacttataa ctgcttttgg ttcaaacact
     4801 ttatcactta aatttaattc ttttagacag tctttaacca atgtaacttg gtctgctgaa
     4861 tcataaatta caaaacttct attgtaacca atcttattta tatcttttct tagtatacga
     4921 acacagcaag aatggaatgt acttatccac atatccttag tttcaggacc tatattttgt
     4981 tcaactctct ctctcatttc atttgcagct ttgtttgtaa atgttatagc taatatattt
     5041 ggtgcttgta cacctttatc ttctataaga tatgctattc ttgttgttaa taccttagtt
     5101 ttacctgaac cagcaccagc cagtataagt actggacctt cagtcttttc tactgcttct
     5161 ctttgagcag gatttaatgt atctaaattc atatttctcc tcctgttaac agccataaat
     5221 atattttaat ctaaaatatg aattacttca aattataatt atgtattata ttaatagatt
     5281 atttccaata tattacaatt aaataacatt tttccatata atttatttaa ttgtttaata
     5341 aaaaaaataa ccttatgatt tggggaatca taaggcacct ttaaggggtc taggttaagg
     5401 gttattatat ataaatctat ttttttaaaa taggtttagg gcttaattat ttatttgtat
     5461 taagttctct ttccttccaa tacttataca aatcttcttc tttcttaaat aagggtaaat
     5521 tcatatttct atgtactttt actaaccatg gggattctag gccagcttgt ataatgttat
     5581 tttcatttac aaatacattt tctgcctttc cttcatcaat taaaatcccc ttatccaata
     5641 catatatata atcgcatatt tcatacatta aattcatatc atgactggat attactattt
     5701 ttatattatc tttattcaat ccctttatta tatctacaat agacctagta cttactgggt
     5761 ctaaaccagc tgtaggctca tcaagcaata taatttcatt ttccattgct ataactgatg
     5821 ctatcgctac cctttttttc tgaccataac ttaaaaaatg tactggtcta tctataaaat
     5881 ctattccatt aacagctact agagctttgt ttattctctc tttaattatt ttttcatcta
     5941 taccaatatt tctcattgca aaggctatat catcatatac ttttgaataa aaaatttgtt
     6001 tttctgggtc ttggaacaca atacctacat tttttctaag gttgtataaa cttctcttat
     6061 catattttaa cttttcttct ttaaaaagaa tttctccaga tgtaggcttt aatataccca
     6121 ttaaattcat aaataatgtt gattttcctg aaccatttga acctattata cctataacat
     6181 ttccctttga aaaatccata ttaatattta atagtgcatt ggtattcttt tcatattgat
     6241 aagttaaatt atttatttta aacatcatta tcccctacta tgtgaaattt accatcatac
     6301 aattttatgt ctaatgatat acacatatca tcataccttg tcataagtct tttgtacagc
     6361 atactgccaa gaattcctaa tgatttatat gaagttttta aatttatgta cccaaatctt
     6421 agttcttgtg acttatatat ttctgaaaat tcttctaaaa atatgaatat aaacctatat
     6481 attaacattg atagctcaac aaaagtatca ggcaaatgca tttttttaaa gaagaatatt
     6541 aattgattaa aaggtgttgt aagtatacaa aagtatacac aagtcaaaca agacatagct
     6601 ctaaataaca catgaataga catgtcaata gatgcttttg atattcctac attgaaacta
     6661 aaaatttcaa aactatataa taagtctgcc ttattaaaag atatatttac aagagttatt
     6721 cctatactaa gaaataaaaa gtacattggt attctgagta attttaaata attctttaaa
     6781 tctattccag ctacacaaac aactagaata ctcattaaaa tcataattcc acttaaaaca
     6841 aatatatttt ttattaccat agaagcaatc agaaagatta ctcctatggc tactttttta
     6901 tttggattta ctttacttaa tctatttgta tatgcatatt tatcaattat aagcataatt
     6961 aacttctctt tcctttttga acacctaata tgtaccctat tacaccagca cctaacgctg
     7021 cttgtgcaga gaataataaa ctttctattt ctccacttgg tggctcatat gggctactaa
     7081 accatggttc ataatctgga tttatcttag taatttcact ctcagcttga ccatctgctc
     7141 ctccatattc agctcccgaa ttaacaagta atgggaatat gataagagct actactaata
     7201 aaagtagtaa tatattagtt ttcgtctttg ttttagcact cattagcttt aactccttta
     7261 tcattatttt ctagtatgct tttttctcca ttttctgtaa ttaaattata tacaattaca
     7321 gtaagaatac cctctgctat tgctattggt atttgagtca taaagaaaat tcctaaaaac
     7381 ttaattgctg aagccatcac tccacctgat gggtctggga aagccagtgc taactgtatc
     7441 gaagttactg tatatgtagc caaatctcct attgctgctg caagaaatac tggcattgca
     7501 ttatttccgt cttttttctt taatgcttta aatattaaga atgatataat tggtcctata
     7561 atagccattg aaaaagcatt agctcctaaa gtagttattc ctccatgtgc caatagtaat
     7621 gcttggaata ttagaactat agttccaaga acaaacatta cacttggtcc aaacattatt
     7681 gctccaagac ctactccagt aggatgtgaa caacttcctg ttactgaagg tatctttagt
     7741 gctgacaata caaatacaaa tgctccacac agcgctaata atactttctt tttagggtct
     7801 tgctttacta tttttcctat tgattttagt cctactaaaa aaaatggtat aaatataact
     7861 ccccatgcta tagaccattt tactggtaag tatccttcca ttatgtgcat tgcattcgat
     7921 gtaactggag ttaaaacgat taacataagt gcagctataa cccctaattt gatattttgt
     7981 ttcattttac tatcccctct ctccctttct aataattaaa atataaataa tatatgtctg
     8041 atttatttta aaaataaaaa taaaaaagcc taactcgatg gctaggcttc taattttcga
     8101 taatccaaat acaaacacat aaaatgaaca tatctgtata attattataa gaaactttct
     8161 ccatccccga agaaattact ttaatatttt aggcaggtct cctgacttag cttcttccta
     8221 ctcctcttcc ttcccatgag atataaaaaa tatcatcata gtggctaaca aaagtttcgt
     8281 ctgcatcaca gtagcggggg ctgtaaagga atttaacctt tttccctatt aatcttgctt
     8341 aaagaaccta taatattata tatttaattt ttaaattatt cttaaatcat tttctatata
     8401 aatcattgta taccttttct gtgaaaattt ctacattttt tataaatttt attcattttt
     8461 atatttttat gtatataaat tcttttttgt aagtttatta atttctaatt atcttcttta
     8521 aatacattga aactacatca gaaaatctag ttatatctct tgaatatata aaatcatttc
     8581 catatattaa tctttcagct tcaagttctg attcttttcc tgtaaatact cctagtacta
     8641 atatattttg ctttctagct tttctcactt cattggctgt atctctcaaa ccaactatac
     8701 ccctataagc cttttctcct cttaatgttc tctcactact ttttcctatc tttacatcat
     8761 ttggtcttcc atcgctgaga actatcagga ttttattttc ttcatctcta tttattagac
     8821 cactacatat agattttatt gccaaaccat ctctattatt accagcacaa aaatattcaa
     8881 atatattttc actatttttt atactatcat cataatcttt aaatcttttt aatattgtat
     8941 agtctaaaaa actagaaaat cccatcactc tatttggtat gccaacttct gttagagcat
     9001 ttgcaattat gtaagcttga attgccacat ttccctgatt tctactttga gagccacttg
     9061 catccaataa tatatcaata acgtatttcc ctttttcatt ttctatatct ttataaaata
     9121 ttttattatt attacttcta ccaactctcc aagctctatt tgctaatata gtaccacaat
     9181 cagaatatac tctactttta gcattttctt ctattaaaat tcttgacaga ctttctttta
     9241 atctctttat ttgctgtcta tacattctag catgttctct aaattttcct aaattatttt
     9301 ctttttgtct tgttacatat tttagttgaa atacattatt acactcactt ctaagaactc
     9361 catctgtaaa gtgtattcta caaccttcat gaacatttct acaattttgt gtctcaattt
     9421 ttttaatttc acctttactc aaataagttt ttccataata atgttctatt tttgaatata
     9481 tcttatttgc agtgtcttca tcaacatata taactcggtc tgctgaattg ctacatttag
     9541 aatcatcaaa atcccctaca ccttctacaa gcatggatga agatattgaa tctatatcac
     9601 tttcaataat attttcttct tcatcactat aaagttcttc atacatgaaa tcagcaaatg
     9661 tatcaaaatt tacttctaca ctttctacat tattttcatc aataagacta ttttcttctc
     9721 catttaatat gtattcgaaa taagttaaat atataacatc taatgctttt aaaatctcca
     9781 tcatatcttg attggtatct atatttctta tatcattaac tattcttcta gtcatactat
     9841 ctaatgctgg gtgcttgtcc atattataaa gtacaaatgt gtatttaatt ttatccataa
     9901 tattatcatt gtgtattttg gtaaaacgtg aaacaatatc tttataagca tttcttctta
     9961 tgtcttctat ccctggccta tctttaatga ccttttcttc aactattgta tttaatataa
    10021 cttgaattag acccagtata atatctttgt tgtatccttt tcttattcta tttgtaagat
    10081 attttttaac tatatcccaa tctacatact ttcttctagc acctgcaatt attgcaaaat
    10141 atagacctat ttcttttgaa gagtaatctt tttctgatat atctatactt tcattgtaat
    10201 caccagctat tgtccatgat aagttattag ctctattttc aaattcataa tcatcatata
    10261 tccatttcat aatctcacct atattaaata tgacttattg gaaatacatc tatactctcc
    10321 catttattag gtattctagt tttaattaca tctccaacca tttctttctc atatacatca
    10381 aaagtctttc ctgtaagtcc catattaatt gcaagagttg gttttaatcc tcttcttata
    10441 gtcttaagag atgccataag accacgtaaa tcaatagctt tactagatat ctctccattt
    10501 tgagacttca attgtaaatc taaaaatatt cctgcaaagt gtattaattt ttcttcatca
    10561 gcatctgaaa attcattttt taaaatcatc attaacttat cttcttctat aggaggaata
    10621 tctattacca taaatctaga taccagtgct tcattaagtt cttttgttcc tgcatattca
    10681 taattcatag tcccaataaa tcttgttgct ggatgtagat tgactctctc atacccaggt
    10741 acatcaatta ttcttctata gtctaaagct gaatgtacca caactatagc atcatttttt
    10801 gccatattaa tttcatcaaa tactccaaaa cctccattaa tagcacactc ataaactgag
    10861 cctcttctaa gtttaacttc attgtctata aatgtatctg tacctataag tgttgaactg
    10921 tctgtattta tatgaaatga tgtattccac tgaggtctat taaatatttc ccctagatta
    10981 tcagcaagta aattttttcc cgttgccttt ggtcccgata ataatatatt ttcttcttct
    11041 aaaatagctg ctatacacat agacaagata tctttaccat aaaaataagt tttagacttt
    11101 gttactctgt attctaaatc tttttctaaa ccataataat ttctaaaata cagtacatct
    11161 tcaataagtt tttcatctac cccttgcacc cttagattat ctattatatc catattttta
    11221 ccccttttat ataaatagta tttgtttcat ttcttatcac cattttagca taaaaggata
    11281 taagctaaaa ttgatttttt aatctccccc attttaagta aaaccatgca ccattttatc
    11341 actacaaaat taagagtact acttactgat atttcttgat tttgttcgtc tatacgtata
    11401 taatatatgc tgataaatta tgaggaaagg atgattgaaa tatggaatat atgtcttgcc
    11461 cagaagtagc aaaaaaatgg ggcatttctg aaagaagagt acaaaaacta tgtgaagaaa
    11521 gtcgcatacc aggagtttca aaactcgggt atatgtggct gataccaaag aacgcagaaa
    11581 agcctgttga tggtagagcg aaaagaagaa aggaagtaca atatgaatag cgttttgatt
    11641 atagatgatg acaaagaact ttgtgctctt atgaaaaaat gtgttgagca agaaaattta
    11701 tctgctatta ctgcacattg tggtgcagaa ggtctacatt tgctcgataa aaatagacac
    11761 acactctctc ttgtaatttt agacataatg atgccagata tagacggttt tcaagttctg
    11821 aaaaaaatta gagaaacaag taatattcca gtaattatgc ttactgctaa aaatgacgag
    11881 gatgataaag tttccggact acgtttaggt gctgacgatt acctaacaaa accgttcagc
    11941 ataaacgaac ttatggctcg tgtcaattct cttattcgtc ggtatactat gttaaaccct
    12001 gcttttataa cagacacaga ttgcataata ataaatggca tgattattga caagacaaac
    12061 cgaattgttt ctatcaataa tattcctgtt caacttacaa gcaaagaatt tgatttactt
    12121 gcttttctgg cttctaataa gggacgggta tttaccaaaa aacagattta tacacaagta
    12181 tgggaagaag aatatgcttt tgacgatagt aacatcatgt ctttcatcag taaattgaga
    12241 aaaaagattg aaccagaccc caaccatcca ttttatattt tgacagttcg tggtgtcggt
    12301 tatcgtttta ataaggaggc ttaacatgaa tacaagtgtc tatctacttt tgatattggc
    12361 tcttagcctg attattatag cgtaccttgt ttctgtcctg ctacgtgtcc gaatacaact
    12421 tacacttata aaagatgctt tagaggatat aaaaaatgga aaccttaatc gccgtatttt
    12481 aacagatgaa aacgacatta caaaacaaat ttgctatgat attaacgaaa tagcaatcaa
    12541 tagccaattt caacttattc agcaaaaaca atcagaacaa gcctacaaac agctaatgac
    12601 aagtctctcc catgatgtaa aaactccact tgcttctttg gtcggttatt tggaagcagt
    12661 agaaagtaaa attgttgtag gagaggaaaa agacgagtat atccatgttg cgtctaataa
    12721 ggctcattac ttaaagaatt ttgtagaaaa tctatttgaa tgggtaaaat tggattctaa
    12781 ggaacaagtt tttcattttg atatttttga cttgaatgaa ttatcccgta atattatatc
    12841 tgattggata cctgttttag aaagcagtca ttttgaatat gaatttgata taccagaaat
    12901 agagtacttc ttacgtatag acgcaaatgc atatacccgt atcattaata acctattaca
    12961 aaatattatt acccatagta gtggcaacaa aatgacattg agaatttttg aaaacaaaga
    13021 gcaagctcaa attataataa cagataatgg gaaaggtata tcttcagaca atctcccaca
    13081 tatttttgaa aggttgtacc aatgcgacca ttcacgagct tcaaagggaa atggtttagg
    13141 cttggcgatt gcaaaggaac ttattaacgc tcataaggga aatattaccg ccaacagcac
    13201 tcccggtatg ggaacagaat ttacaatatt gcttcctaaa gccctgtaaa agtacgaggc
    13261 tttcttactc taaaacaaga taaaagcaag attatggcaa ggttttggca aggttcatgt
    13321 tttataattc taattgtagt tgattttgac gttgtatcaa ctatcttttg tagaaacaaa
    13381 tttattaaaa gtgaggaaat tatatatgaa tgattttgtg attgaaacaa agcaacttac
    13441 aaaaatttat gggaatcaaa cagtagttga caaagtcaat ctccatgtaa aaaaaggccg
    13501 aatatatggt ctgttaggac gcaacggagc tgggaaaacc acaattatga aaatgatttt
    13561 aggattgact tcaatcacgt atggcgaggt agatgtattt ggagagaata ttaagggatg
    13621 tgaaaaacga gtatatcccc gtatcggtgc aattatcgaa acgccaggat tttatcctaa
    13681 tctgacagga acggagaact tggagatttt tgcaaaactt cgtggtacag ccgcccctaa
    13741 ttctgtaaaa agagcattgg agattgtagg cttaccttat aaaaataaaa agctgtttag
    13801 taaatattca cttggcatga aacagcgact tggtattgcc aacgctatcc tgcatgaccc
    13861 ggaactgtta gttttagatg agccaaccaa tggacttgac cctattggta ttgcggagat
    13921 gagaaagttt attagggact tgagcgttga gcatggaaag acaattctta tctccagtca
    13981 tattctttca gaaatctcat tgttggctga tgatattgga attatcgacc acggtgttct
    14041 tttggaagaa agtagtatga gtgaactaga aaagaaaaac agtaaatata tcctgctaca
    14101 agtttcagat attccaaaga catcacttat tttagagcgt caatttaaca cgaaggatta
    14161 ttccgttcaa gatgaccaca ctttacgcct ttataacact actctggata tgggtgaaat
    14221 caataaagca cttgtaatgc agaatgtaac tgttattagt tctcaacttt gtaatgatac
    14281 tttagaggac tactttaaaa aaatcacagg aggagagggc attgcttaaa cttattcaaa
    14341 cagaatttct taaactgcgt cgtaggaaac ttatttggct gatgttgttg tctgctctca
    14401 ttatgccgtt tttcgcacta ctatacttca attattttgg taaaacaggt gttgagccaa
    14461 caaagtttta taaatggtct gcatttggtt atacactttg gattattttg cccgttgttt
    14521 tgggtatatt ttgcaccatg cttatacacg aagaaaacca gtatgatatg ctcaaacagc
    14581 tttggattgt acctattagt aaaatgggat acttttttag taaatttttc gttgttttga
    14641 tttactctat ttgttttatg ctgattacag ccgttgcttc tgtgttattt agtgtacttt
    14701 ctggatatgt cgtatttagc tgggcaagca ttttatattt gttgaaaaaa tgtttagaaa
    14761 ttggtgtaat cactgctttt gtaatgttgc cgattttggc aatcgctaca tctcaaaaag
    14821 gatacattct cccaatttgt atcacattgg tatatgcctt ttccggtttt gttataatga
    14881 caataaatat gtatttacac ccattatcca gtatgggagt tattatcatg agaaataaag
    14941 acattccagg tcttatattt tcacaggaaa ttaatgtccc attagcattt ctttgtattt
    15001 gtatttggag tattgtatca gtattgtttg caagcattgc actaggaaga agaaagtgag
    15061 gtgtagataa tgtggaaatt actttgggct gaatgtcaaa aaattcgtcg ttcaaaaatt
    15121 gtttggatta cattttttgc atccattatg atagcagtca tagtttttgc aggagggcag
    15181 gatgtatata atgacccaga tttacattat ggcttaaaga gcgtttatga cggttcacgg
    15241 tatatagaca atgcaggttg gtatatggac gcggtacaac cttggtcaac tttttttgtt
    15301 cttcctgctg tcattgccct attaggaagc tatattattt gtcgtgaaga agaggaggac
    15361 actattaaat ccctgcgtct tattcccata aatgaaacaa aattaaccat agcaaaaatg
    15421 attattactt ttatattcag tatacttctt tatttgttac tctttactat aacatttttg
    15481 actgaatccg ttttacattt ttccgattta tcgacaaaac tggttttaag ttgcatgaaa
    15541 gagtattttc ttgatggcat aggtgtattc tttgcaattt ctcccattat tgccttggta
    15601 tcgcgcatga aaaaaggata ttggcttgca ttagtattca cagaagttta ttctattgct
    15661 ggactatttg cgggtatgtc aaatgtctta caaacctttt ttcctattgc agcaatattt
    15721 aatatttctg gatactatat aactactgga gaaaaaatta tggggagtat cataagttta
    15781 ttactttgtg cctgcctttc tgcatttata ctgaaagggc taaaacacaa tgaaaaaaat
    15841 tgaaataggt atatcaaaat gaaaaagata aaaaaactga ttattctaat tgtattcttg
    15901 tttctttttg gcatgttaca ctgttatact gaatggaaat tcaacaatga cccacctaaa
    15961 ggggtaatag aaagaataaa tgagtggact gataagattg gactggatta gtaacttatg
    16021 ttaattatag tgaatgtaat attagcaagt tattgtttcg tattttaata ctataacttt
    16081 aaatatttgg ttttaatctt ccgaacgaat ataaaaaaac tgtcacagag gcaacatatt
    16141 tttgaatatt tataaagtgg tagttttgat tttcaaagct gccgcctttt cttctatata
    16201 agcaaactta tcctcaacaa agtgtaaaac atggtacatt ttaagaaaaa agtaccacag
    16261 acacaagcaa taaaagaaac gccgagaacc tacaaaaacg tagtgcttat gcagatttac
    16321 gacgccttct attttctcaa ccgtcttaat gaccttgtac aaaagaaatt tttctaatat
    16381 tttttataat cttatcttgt gatgtttttt atgtataaat gacttaataa caaatccaat
    16441 aaaaattatg gatattggta taattagaac gaggaattta taatcattat aatgtgacat
    16501 tattcttacc caactctcag aaaaaatata tcctatactg attaatgaag tattccatat
    16561 ggttattccc acgatagatg atattacaaa agttataata ttttgacctg ctatacctgc
    16621 tataaaagat atgtatgttc tacataaagg gattaatcta catacacaaa cagaaataga
    16681 agagtattta ttaaaataat cttgagatgc aaatattcct ttctttgcct ttggacatat
    16741 ctctattatt ttatttatga tagatttacc tccccaattt cccactgtat aacaaaatat
    16801 cgaaccaagt atacctgcaa ttatacttaa aattaaaatt gtcagaaatc caaaatgatt
    16861 tcttgcagca attgctccac ataaaggtaa aacaacttcg ctaggtaatg gaaaacaagc
    16921 atactcaatc ataattaata caaatacaga gattaatcca tattgtaaaa taaaactatt
    16981 gattacagac acttacttat ctatctcctt tcttatttaa aggttttaag ggtacatatt
    17041 ctaaggattc tcaccctata tttaaagaat caatctcttt aaactattat atgtatttac
    17101 acaaagcatt tataacatac tttaaaaata gtaccttaaa ataagaaact taataaataa
    17161 aaaaagatta cgtggctacg tcctactctc ccaggcag
//
LOCUS       DAMJWP010000470        16359 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.guided.072, whole genome shotgun sequence.
ACCESSION   DAMJWP010000470 DAMJWP010000000
VERSION     DAMJWP010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16359
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.guided.072"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(116..808)
                     /locus_tag="PC700_003800"
     CDS             complement(116..808)
                     /locus_tag="PC700_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDF2583006.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCKEGILRAVPYSSPIVEGFKKVDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESEATKLAILNATSKELQKIKYTYLKSIEELDKVSHGLIEECYDADLQFHKEIILAS
                     HNPIFIKYYELIPKTVSSNQYFGFKYRNSLKDMISHHNKIMMAFDSKDSYLGYTSMYN
                     HLEDVIQLFQHD"
     gene            complement(834..2021)
                     /locus_tag="PC700_003801"
     CDS             complement(834..2021)
                     /locus_tag="PC700_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2583007.1"
                     /translation="MKKISNQKLLLLSIVTFFWFSQYVYIPFQNPYLSACNVSTNMIG
                     IIIGAYGISQCLLRIPVGVFADSINKHKIFILSGIFFAGLASLIRVFSPGGNGFLIAN
                     ILSGISSSTWISYMVHYTNFFSTEHQQKATSTIILVNNLGMLIGFVMSTLFYEKLGMT
                     ALCIMSVLSSVIGTILALNIKNHNSKKIPEKISIKKYLSICKDKRLVFFSFMALIQQG
                     IQMSTTMSFTTQILKNLGSSSTLIGLSSISYMVCAVASSGFASTKFCTKYGPKFWIPL
                     VFILVSAYCFLVPTIKNINIIFVLQVLPGISTGILLSYLTSESMKNVPKHQKSTAMGF
                     FQAIYAIGMSVFPMIVGKISEYTSIQYGYFILAIIAILASILSYTYYKKVDLTLNYDI
                     IQS"
     gene            2357..4234
                     /locus_tag="PC700_003802"
     CDS             2357..4234
                     /locus_tag="PC700_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2583008.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKTEGVITVVESMGQIQVVIGNKVKTVYDEVIKIVPQSNDVGKKNESQEKQSIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYVEKVLEKYIPEVIKIIMVPTLSLVVMLPATLFLFGPIGIYIGNVI
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGAA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AFGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSFIKVETKDGVIDITSPVEGECIELSEVKDDVFASKAMGEGIAVLP
                     TKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKKAGYDVTTPVIVNNTFDFGQVVSCKSSYVSTNDNIISLVLAG
                     N"
     gene            4546..5952
                     /locus_tag="PC700_003803"
     CDS             4546..5952
                     /locus_tag="PC700_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDF2583009.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLDFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNTVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEVLDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            5980..6828
                     /locus_tag="PC700_003804"
     CDS             5980..6828
                     /locus_tag="PC700_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2583010.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            7489..8181
                     /locus_tag="PC700_003805"
     CDS             7489..8181
                     /locus_tag="PC700_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2583011.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            8178..9113
                     /locus_tag="PC700_003806"
     CDS             8178..9113
                     /locus_tag="PC700_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2583012.1"
                     /translation="MKMDEQTIILFLFLLSIGLLFLSIFAFSKLGHIHKRLKDIEEIL
                     ADVQIGNENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIGQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVDDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQISVESEIGKYTVFTVSLPLI"
     gene            9261..10184
                     /locus_tag="PC700_003807"
     CDS             9261..10184
                     /locus_tag="PC700_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2583013.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRKFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAHAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            10168..10938
                     /locus_tag="PC700_003808"
     CDS             10168..10938
                     /locus_tag="PC700_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2583014.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVPKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVTVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPLLSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            10948..11730
                     /locus_tag="PC700_003809"
     CDS             10948..11730
                     /locus_tag="PC700_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2583015.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGIAVLLSLFSFLCTFILGLLFCNINISVTLIVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            12107..12643
                     /locus_tag="PC700_003810"
     CDS             12107..12643
                     /locus_tag="PC700_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HDF2583016.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            12675..13064
                     /locus_tag="PC700_003811"
     CDS             12675..13064
                     /locus_tag="PC700_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDF2583017.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            13101..13487
                     /locus_tag="PC700_003812"
     CDS             13101..13487
                     /locus_tag="PC700_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HDF2583018.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGSQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            13614..15056
                     /locus_tag="PC700_003813"
     CDS             13614..15056
                     /locus_tag="PC700_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDF2583019.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVENESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLIDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            15353..16168
                     /locus_tag="PC700_003814"
     CDS             15353..16168
                     /locus_tag="PC700_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2583020.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINNFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKRVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEIRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
BASE COUNT         5920 a         2026 c         2986 g         5427 t
ORIGIN      
        1 tgaaatgatg aaaatatatt tgatttcata caaaaaagag tgcctcataa ctaaaaagtt
       61 cactttgaga cactccattt ttatatgtta gttgcattta aggtaaaata gaagtttaat
      121 catgttgaaa taactgaatt acatcttcta aatgattgta cattgatgta taacctaagt
      181 aagagtcttt actatcaaat gccatcatta tcttgttatg gtggctaatc atatccttaa
      241 gagaatttct atatttaaaa ccgaagtatt ggttagaaga aactgtttta ggtattaact
      301 cataatattt tataaaaatt ggattatgag aagctaaaat tatctctttg tgaaattgta
      361 agtcagcatc ataacattct tctatcaaac catgagaaac cttatccaat tcctctattg
      421 actttaggta agtatatttt attttttgaa gttctttaga tgtagcattt aaaattgcta
      481 gtttagtagc ttcactttcg ataagttgtc ttgctcttaa agattctaca taaaattctt
      541 gtatgttatg atttttatag atttcaccat catcaaatag gtcaactttt ttaaatcctt
      601 caactatagg acttgagtaa ggcacagctc taagtattcc ttccttacag agtatattta
      661 tggcatttct tacaggtatt ctacttatgt taaataggtc agcaatcttt ctctcacttg
      721 gtaatttact attaatttta aaagttccat ttagaatgtt taatttaata taatcaataa
      781 ttttttcagt ggcatttaag cttttcataa aaactcctaa acatataatt tttttatgat
      841 tgtattatat catagttaag agttaaatct acttttttat aatatgtata agacaggata
      901 gaggccaaaa tagctataat agccaagata aagtatccat attgtattga agtatattcc
      961 gatattttac caactatcat tggaaataca gacattccta tagcataaat tgcttgaaaa
     1021 aaacccattg cagtagattt ctgatgtttt ggtacatttt tcatagactc gctagtaagg
     1081 taagaaagga gtatacctgt agatatacct ggtagcactt gtagtacaaa tattatatta
     1141 atatttttta ttgttggaac taaaaagcaa taggctgaaa ctaaaataaa aactaaagga
     1201 atccaaaatt ttggcccata cttagtacaa aatttagtag aagcaaagcc agaagaagca
     1261 acagcacaca ccatgtaaga tatagaagat agacctatga gggtagaact agaacctaag
     1321 tttttaagaa tttgagtagt aaatgacatt gttgtagaca tttgaatacc ctgttgaatt
     1381 aatgccataa aggaaaaaaa tacaagtctt ttatctttgc agatagataa atatttttta
     1441 attgatattt tttcaggtat tttttttgaa ttatgatttt ttatatttaa ggctagaatc
     1501 gttccaataa ctgaagacaa tacactcatg atacataagg ctgtcattcc taatttttca
     1561 taaaataaag tactcataac aaatccaatc aacattccta gattgtttac caatattatc
     1621 gtactcgtag ctttttgttg atgttctgta gaaaaaaagt tggtatagtg aaccatgtaa
     1681 gaaatccaag ttgatgatga tattccagat aaaatgttag ctataagaaa accattgcct
     1741 ccaggagaaa agactcttat aagagatgct aaaccagcaa agaaaattcc agacagtata
     1801 aatattttat gtttatttat agaatcagca aatactccaa caggaatcct taataaacat
     1861 tgtgagattc catatgcacc tattattata ccaatcatat ttgtagatac attacatgca
     1921 cttaaatatg gattttggaa aggtatatat acatattgtg aaaaccagaa aaatgtaaca
     1981 atagaaagta aaagtaattt ttgattagat atttttttca tattatcctc catatggtat
     2041 ttttataatg caaataatat accatttaga ttttttaatc aagtactttc tctattaaaa
     2101 aaaatttaaa tatataatta ttatttacaa aaaaattata ctttatagtt gaattaagaa
     2161 aacgcttatg ataatataaa gttaacaaat ttgaatatat aacagtagac gtgttactgg
     2221 taatgcaggc aagatcgaat caatttttat agattatcta tagagtacta tgggtataat
     2281 ctatttattt ggttcgattt ttttgtttaa ttggccataa aacaaaaagc ctatagttga
     2341 aaatggagga ttttttatgg attataaaaa tatagctcaa gaaattctac taaatgtagg
     2401 aggaaaagag aatgtaaatg aagtgactca ttgtatgaca agacttagat ttaaagtcaa
     2461 aagcgcttct aaagtgaata aggataaatt atctaaaact gaaggagtaa taactgttgt
     2521 tgagagtatg ggtcaaatac aagttgtaat tggaaataaa gtaaaaacag tatatgatga
     2581 ggttataaaa attgtacctc aatcaaatga cgtaggtaaa aagaatgaaa gtcaagaaaa
     2641 gcaaagtata attaattcta tattatcagc agtagctggt atatttacac ctacaatacc
     2701 tgctatagca ggggttggta tgattaaagg tattttatca gttcttgcta tgtattatat
     2761 gaataaaaat ggagtagata taaaagaaac gcaaagttat attatcttaa atgccatggc
     2821 cgattctata ttctatttta tgcctattat actaggatat actgcggcta aagtatttaa
     2881 tgcaaataaa ataatatcta tggtattagg agctacactt tgttatccaa cttttactgc
     2941 tttgatggca ggtgaagaat ctgttagatt tttaggattg gcagtaacaa aagctacgta
     3001 tacatcatct gttattccaa taattattgc tatatgggct ctttcatacg ttgaaaaagt
     3061 attagaaaag tatatacctg aggtaattaa aataataatg gttccaactt tatcattggt
     3121 tgtaatgtta cctgcaacat tatttttatt tggaccaatc ggaatttata taggaaatgt
     3181 cataaacttt tcttataagt atatttatga attaagccca gcgttgtgtg gagcatttgt
     3241 aggtgggtta tggtgtgtgt tggttatatt tggtgctcat agagcattat taccaatagg
     3301 tataagtgat gttgctcaaa caggaagaca gaatttattg gcatttgcag gagctgcaaa
     3361 cttttcacaa gcaggggctg ctctaggagt attctttaaa actaaaaatc aaggactaaa
     3421 aacaatttct atgtctgcaa ctataacagc tttatttggt ataactgaac ctgcaatata
     3481 cggtgccaat cttagattaa aaaaacctat ggtatgtgcc gtaatatgtg gtgcatttgg
     3541 aggtgcgata atgggaatgg gtggagctta tggtaatgca tttgcaaacc aaggtgtttt
     3601 aactatacct gtatatgctg aagcaggtgc attaggtttc ttaagttatc taggtggttg
     3661 tgctatagca ttttttggtt cagctatttc aacttatcta gttggatttg aagatttgga
     3721 agagtcttct aataaggaaa attcatttat aaaagtagaa acgaaggatg gtgttattga
     3781 tataacttca cctgtagagg gcgaatgtat agaattgtca gaagtaaagg atgatgtatt
     3841 tgcttcaaaa gctatgggag aaggtatagc tgtgttacct acaaaaggag taataacagc
     3901 ccctactgat tgtgaagtag cttctttatt tccaacatta catgcaatag gattaaaatt
     3961 agataatgga gcagaaatgt taatacatgt tggaatcaat acagtagagt taaatggtaa
     4021 atactttaca aaacatgtta atcaaggaga tttagtgaaa aaaggtgata aacttatatc
     4081 gtttgatata gataaaataa aaaaggctgg ttatgatgta actacgccag ttatagtaaa
     4141 caatacattt gattttgggc aagtagtttc ttgtaaatct agttatgttt caaccaatga
     4201 taatataatt tcattagtat tagctggtaa ttaagttaat ttgactagag ataagtaaat
     4261 tgatttaaaa ggaaataagt aaatcaacaa ttaaatacat ctaaaagtaa gtttattgca
     4321 atgaattgtt aaagaaaata attaatatgg agtaaagcta ttgcaatgaa gtaacaaaga
     4381 aaataattaa tatagagtaa gtttattgta atgaattgtt aaagaaaata attaatatag
     4441 ggtaaatcta tctcaatgaa ataacaaaga aaagagttaa tatagagtga acttattgca
     4501 atgaatcaac aaaaaaaata atcaatataa aaaggagaaa tatttatgaa tacaggattt
     4561 ccaaaagact ttttatgggg agcatcatct tctgcatttc aagtggaagg agcttgggat
     4621 aaagacaata aaggaaaaac cgtagccgac tataattctt ttaaaaagtc tcatttacag
     4681 gcagatacta aagttgcatc agacttttac cataattatg aagaagatat tgaattaatg
     4741 aaagaattag ggatgaaaac atatcgtttt tcaatttcat gggcaaggat aataccagat
     4801 ggtgaaggag agataaacca gaaaggttta gacttttata ataagataat agacaaacta
     4861 atagagtgtg atattgagcc attcgtaact ttataccatt ttgaccttcc atttaaatta
     4921 gtagagaaat ataatggttg ggaaagtaga gaaactgtct atgcttttga aagatttgct
     4981 aaaatatgct ttaagcattt tggagatagg gtaaagtatt ggcaacctca taatgaacag
     5041 aatttaattg ttagagtgga ggagagaatt aatatatatg atgaaacaga ttcatggaag
     5101 atagataaaa tgcgtgctca gatggattat aatctttgct tagcacatgc attagcagtc
     5161 aatgcatgtc atgagatgat aaaagaaagt aaaattggag cagcagtatc atcatctgta
     5221 acatatcctc ttacttctaa gccagaagat gtatatgcag ctagaatgaa tgataatttt
     5281 aaagtgtact atatgcttga tatgcatcat tatggagaat atccagggta ttatatgaag
     5341 tatcttgaaa aaagaaatac agttcctcac atggaagatg gggataaaga gatacttaaa
     5401 aaagctaaaa tggattttat agctgtaaat tattatagga caaattgtgc agaagcttta
     5461 cctgaagatt cacaacatcc atttggatta agagaaggaa ctgttgattt tagtatgtat
     5521 ggactattta aaatgtcaat gaatcctaac ttagaggctt ctgagtatgg agcagcaata
     5581 gacccatcag gtcttagagt tgccttaaat gaatattggc aaagatatca cttacctgtt
     5641 ataataacag aaaatggtct aggagctaaa gatatattag aagatggaaa aattcatgat
     5701 gactatcgta tagactattt aagaagtcat ataaatgcat gtaaactagc tattgaagat
     5761 ggtgtagaga tgattggata ttgtccatgg tcatttacag accttttaag ttcttctcaa
     5821 ggttttaata agcgttatgg acttgtttat ataaatagaa ctgaccatga agttttagat
     5881 ttaaaaagaa tcaagaaaga tagtttttac tggtataaag aagttataga gaacaatgga
     5941 atagtaaaat aaacataaat atatattgag ggttttagta tgtacattac aaaggtatta
     6001 aataattcgt tgctacttgc aaaggatgat aatgatgaag aaataatatt gatggggaaa
     6061 ggcattggcc ataattataa ggccgggtct gagttaaata aaaaagatat agaaaagatt
     6121 tttgttcttc atgatgaaaa tataaaaaag agtattatac aattggcaac agaaatagat
     6181 gcagaatatt ttggtatagc tcaaatgata attgcatatg gaatagaaaa atataactta
     6241 aaacttatga accatatata tttggcctta actgaccaca ttgcctttgc agtgagacgc
     6301 tttaaagaag gtataatgat tgaaaatcac tatttgttcg aaatcaagga atttaatcca
     6361 aaagagtatg atataggaaa atatgctatt actgttttta aggaggttct tggtttagac
     6421 ctaccagaag aagagattgg taatatagca ggccatttta taaatgcaca acaagataat
     6481 ccttatagtg atagaaataa gagaagtgct aaaattgtaa atgctatttt gcaaatagtt
     6541 cattatcact tttctattgt atataataaa gagtcatttt attataggag atttgtaatg
     6601 catctaaagg ccttttcaca aagatttctg gctaatgaac catcaagaga aaaaatagat
     6661 tttatatatg agcaagttca aaaaaattgt aaggaagagt atgaatgtgt gaaaaaggta
     6721 ggagcttata tgtataagga atactccaga gaattaccaa gacaagagga gttatatttg
     6781 atgattcata ttcataagat tttaggagaa ttatgcgaaa atagttagaa tatcttaata
     6841 tgctagtaat attataaaaa attaaaaatt aactacactt taaatatgaa caattgcatt
     6901 acttaagggg gttgtcttaa aattgatttt tttaattaaa tttgaagctc aactcctttt
     6961 ttaaaccttt atatcattac aactttaatg tttatacgcc tgtactttag atgtatatct
     7021 actttttaca tttctcaaca tgcaaaccta ccattttttg cagtatgtta attgaaataa
     7081 accattgagt taaccctaaa tttattctgg acttatagga atagttccaa agatacttta
     7141 aaatattttc tggaataatt gcttatttca catatgtttt attgtgatgt tttgatttaa
     7201 taaattcttc aactctgtat atagtaaatg tattatcatg tttgttttat tcaagcacac
     7261 ttataatacg cttgattttt tcacaatgat gaaatatact aatgggtgtt ataggtataa
     7321 gaatcgaatt acacttgaag aagtaagtga taaatgagat ataaatcctc atatgataaa
     7381 ctactatttc attgaggaac atattctaag tgatgtcaag atactacaat atgactaaat
     7441 tcctagtgat aaaaaagtct acgaatgagt gtagaaaggg gaactaagat gaataaagtt
     7501 ttaattatag atgatgataa agatctttgt attttgttaa aaaagagtat ttcaattgaa
     7561 cagattgaag caaactattg ccattctggg agagatggac tggttgaact ttcaaaacaa
     7621 gattatcagt tagtagttct agacgtgatg atgccaggta tggatggttt tgaaactatg
     7681 gaacaaatac gaaaatatag cagtattccc atcttaatgc tgacatcaaa aagcgataat
     7741 tacagcaaag tgcatggatt gcgagctggt gcagatgact atctaaccaa accatttgaa
     7801 attacagaat ttactgctcg tgtactgtct ttaatccgac gttacacccg ttttaatcca
     7861 aaaaatgagg aaccacagca actttctttc aaaggtttgt tgattgacct tgaccagcat
     7921 acagtgacta ccaatggcga acctatgttg cttccaccca aggaatttgg tgtgcttctt
     7981 tactgtgcaa ggcatcaggg taagattttg acaaaacagc agatttatga agctgtgtgg
     8041 ggtgagccat atgtatatga tgacagcaat atcatggcta ttatcagtcg cctaagaaaa
     8101 aaaattgaac tagacccatc atctcccatc tatttgcaaa caataaaagg aattgggtat
     8161 caattcaaca cggaggtatg aaaatggatg aacaaacgat aattcttttc ttattcttgc
     8221 tgtcgattgg tctacttttt ctttccatat ttgctttttc caagcttggg catattcaca
     8281 aacgcctaaa agatatagag gagatactgg ctgatgtaca aattggaaac gaaaatcgca
     8341 aaatactaat taagccatgt gatgttatgg ctccattggt ttatcagctt aatgagattg
     8401 tctacgatta tgaaaataag ttactttctc tgaaaaagtc ggataaagct agcaaacaac
     8461 ttatgacaag tctatctcat gatgttcgta caccattaac aactctgatt ggttatctgg
     8521 atgctgtaca cagtggtata gttatcggac aagaacggga ggaatacctt gaaatcgcta
     8581 ggcgtagagc ctacgacctc aaggattaca ttgatgttct ttttgactgg tttcggttga
     8641 actctgatga atttacgctg tcaatagaat ctgtggaaat tgcagagttg tcacgcaata
     8701 ttttgaaaga ctggattcct atattccatg aaaagaaact gaattttgaa attgatattc
     8761 cagaaaatcg actaatggta aatctcgacc cagatggata ttcccgtgtg gttaataatc
     8821 tggttcaaaa tgttcttgct cacagtaagg ctcggcaaat caagattacc atgagtgagg
     8881 attccagaat ggtattactc cgtgttgatg atgatggtgt tgggattgcc agagaaaatt
     8941 taccacatat ttttgagcga ctgtataagt gtgataaagg acgttcagaa aaaggaagtg
     9001 gtcttggtct atctattgtc agtcaaatgg tggagcgaat gggtggtcag atttccgtag
     9061 aaagtgagat tggaaagtat accgtattta cagtgagctt accattaata taaaattgtt
     9121 tttcccccat gtgacatgta tacacagggg gattttttgt aagttataaa gttgcaaggt
     9181 taatacaagg ttgttgcaag gttaatgtaa gattgaggtg gtatgatagc tttaataaac
     9241 aaaggaggat tttattattt atgaataatt taattattga aacaaaaaat ctcactaagc
     9301 aatatggtgc tcaaaagagc gtttctaatt tgaatattca cgttcagaaa ggtcgcattt
     9361 acggattgct aggaagaaac ggtgcgggta agacgacaac tatgaaaatg ttattgggtc
     9421 taacaaaacc aactactgga gaaattaaaa tatttggtca ggacattcac aataacgaga
     9481 agaagcttct tcctcgaatt ggatgtttga ttgaatcacc agggttttat cctaatctga
     9541 caggaacaga aaatctcaaa atctttgcaa ggcttagagg aataccaaaa aaaaatgcag
     9601 tagaatcagc actggatgta gtcggtttgc caaacaatga caaaaagtta ttttctcaat
     9661 actctcttgg tatgaaacag aggttggcga ttgctcttgc cattatgcat gacccacaac
     9721 tgttgatttt ggacgagcct atcaatggac ttgatcctat tggtattgca gaagtaagaa
     9781 agtttattcg tgatctctgc acagaacgag gtaaaactat tttgatttcc agtcacattc
     9841 tttctgaaat tgccttgttg gcagatgaca ttggtattat tgaccatggt attttgttgg
     9901 aggaagaaag tcttgcccaa ttagaagaaa aaaacagtaa atacatacat tttgctgtat
     9961 ctgatgcagc acatgcagca aggcttctgg aaacccaact taacctgaaa aatttcaaag
    10021 tagcagataa ttacaatatt atgctatatg atacagatgt ttctgttgca gaaataaatc
    10081 gtgctttcat tctaaacaac attgaggtat cagaatctca tttatgcgaa gatactcttg
    10141 aagattactt caagaaagta acagggggtg aaggaattgc ttgatataat ttcatgtgaa
    10201 ttttcaaaac tcaagcggtt aaaatttata ctgatttcta ttttaggtgc ttgcttattt
    10261 ccaattcccg caacaatttt gattgcaaag gacaacttgc cctttgagca attgttcaaa
    10321 ttagttgtca attttggata ttttttgcta ttaccaattg tattaagtat tgttgctagc
    10381 caactatttt ttatagaaag agataacgat gtattaaaaa atcttgcaac agtgccagtg
    10441 ccaaaaggaa aactggctct ggcaaaattg gctgtgttgc tgtttatatc attgttctac
    10501 tccgttgcag ggttaggagc tactatcatt ggaggtttca ttgttggaat agtagagggg
    10561 gttactgtaa aattaggaat gagcattgct tttggaatta tgttttttgt tactgtactt
    10621 cctgttgtgg tactcattgt atatttcaat cgcagctata ttttttctat tatactttct
    10681 tttgtatatg ctattttcaa ttttagtata tccttaaata ttatcaattt tgaaccaaat
    10741 aacccactat taagcgtatt acctgctcct gttataatgc gttggtggat ggcattttgg
    10801 ggagatccca ctggcgaata cacggcactt agacagccat atttgctctc aactcctgct
    10861 tgtgtaggta ttttatgttt gataaccatt atagcagtcc tgctgatttg tatgatatac
    10921 aaaaagcagg agaattagga ggtatctatg cttgatataa ttactacaga atttcaaaaa
    10981 ataaaaagat ataatattct atggattggt gttgttgctg ttctgttttc agcactattg
    11041 gcagtttttc aggttgtaag ttcacacggc agtgaccctc taacgtatga gagctttgca
    11101 aacggagtta tctggaataa tttcagtctt agttttccat ttgggattac acttattgga
    11161 ggatatctta ttaaccgtga atatacagac caaaccttaa aaaacatcct aactgttcct
    11221 atttcattac gcaaattgct agtgggaaaa ttaattgcgg ttggaggaat tgctgtattg
    11281 ttaagtcttt tcagcttctt atgcacattt attttggggt tgttattttg caatatcaat
    11341 atatctgtta cactcattgt aaaatcctta gttcagattt tgtctgttaa tctgtattgt
    11401 tatgttgctg ttttgccgat tattgcttat tttggtcgta aacaaaatgc atttctgacg
    11461 ggtgtaggga ttgcatttgt atacgggttt tgtggtgtgt ttgttgctgg aagaaatctg
    11521 acagactttt atccaatcac agcaggtctt ggtctagttg gatacaacaa tggtgctggt
    11581 actgtttatc agccacttat cggtgctatg actttgattg taattctaat cttgacaact
    11641 attctacttg tatttacacc taattatgat aaagttatga ccatatctaa gaaaaaaggg
    11701 ataaagcata aaaagtattc tcataaataa cataaatatt gtttctatta tttaagttgc
    11761 tagacagtga aaagaaaaaa tctgtcgtac agtggctata tgctcatgta ttcaagaagc
    11821 tacagttttg atttttcata actgtagctt ctttttgtat ttattaaatc acggctatca
    11881 taatacttgc aataggtata gcgacaagag agagtactgt catgtgtgtt ttatatatta
    11941 cattaagtat attctaaaat atatgtttta aataatatat ttgaaaatag gaatatttaa
    12001 ataaaaaaaa taatattata tatagttgac aaaaattggc aaatgatata atatataaat
    12061 atagaaatag taatcattac tattattaaa aagggaggaa ttaattatga acttaaaagg
    12121 aactaaaaca gaaaagaatt taatggcagg atttgcagga gagtcagaag caagaaataa
    12181 atatacatac tacgcttcaa aagcaagaaa agaaggttat aatcaaatag ctgctatatt
    12241 tgaagaaact gctaacaatg aaaaggaaca tgctaaatta tggtttaaac ttttacatga
    12301 tggtatgcct tcaacagagg aaaatttaaa ggatgcagca gcaggtgaaa actatgaatg
    12361 gactgatatg tatgcaaaat ttgctaaaga agctagagaa gaaggatttg acaagatagc
    12421 ttatttattt gaagctgttg gaaaaataga gaaagagcat gaagaaagat acttaaagct
    12481 tttagaaaac ttaaatgaag gaaaaatatt taaaagagat gaagaagttg tttggcaatg
    12541 tcaaaactgt ggtcatgtgt atgtaggtac tgaagcacca gaaaaatgcc cagtatgtga
    12601 ccatccaaaa gcgtatttca atataaaagc tgaaaattat taaaaattat cattattaaa
    12661 aaggaatgtt aactatgaaa ttttctaaac aacgagaact gattttaaat gaaatattaa
    12721 ataatccggt tcatcctact gcggattacc tatatgaaaa cttaaaaaaa gataatccaa
    12781 atttaagctt aggaactgtt tatagaaatt tagctcagct aacggaacat ggctttataa
    12841 gaaaggttag tattccaggg tatccagata gatttgatgg tcgaatagat aatcactatc
    12901 atatcatatg tgaagtatgt ggagaggtat acgatttaga atccgaagtt cttaataatt
    12961 tacaagaatt aatatctgag gagacagaca taaaaataac atcttataac ataagtttta
    13021 aagggatttg taataattgt aaaaggtgta gccaagtagg ttagttttca aattctaaaa
    13081 ataattaatg aggtgagttt atgtgtagtg aacaaaaatt ttttatatgt gagacttgtg
    13141 gaaaccttgt aggaatgata caaagtggtg gtgtacctat tttctgttgt ggaaaaccta
    13201 tgaaggaact tgttcctaat acaacagatg cagcagttga aaaacatgta cctgtaattg
    13261 aagtggatgg aaataatgtt actgttaaag taagtagcac aactcatcca atgactaaag
    13321 aacatcatat agcatgggtg tatcttatga ctgaacaagg tagtcaacgc aaatgtctag
    13381 cagtagatgg agaacctgta gttaaatttg ctttgaatga tgatgataaa gtgatttctg
    13441 cctatgctta ctgtaattta catggattat ggaaggctga actgtaatat ttaaataaat
    13501 tgagtcaaag aggaggctat aaatgtattt tcatacaaat agcctcctat aaatttatat
    13561 aaattcaagt tttaattttg tttaatgtca atataatata aggagaaaat aatatgtcaa
    13621 tttataaatg tagtgtttgt ggatatattt atgatgaatc taagaatgat aaaacatggg
    13681 atgaattaag tgaagattgg gaatgccctg tatgtacaaa aggtcgtagt tattttggaa
    13741 agataagcac tgtttactat gaagaagatg aaaagatagc agaagacata gttgaaaatg
    13801 aatctaaatt aaatactgaa aaagagggag atttaaatta cttaagtaca tatttaagaa
    13861 gagatgatga agttgagaaa catatggata taattcacga aatggctgtt acaggaaaat
    13921 ctataataga acctatgaga actaaactac cagtaatttc ttgggatgat attttaatta
    13981 tgggtgctca attaaatcca ttacctttaa atgagcatga tgaagtaaat acaacaacaa
    14041 taataggaaa aaaagcaaag aaacctatga ttatagaaaa tccagtttat atttcccaca
    14101 tgtcatttgg agctttgtct aaagaactaa aaatagcttt agcaaagggt gcagcccaaa
    14161 ataagacagc tatgtgtagt ggtgaaggag gtatattacc agaagagaag gaagcttcat
    14221 acaagtatat ttttgagtat gtaccaaata aatatagtgt aactgaagaa aatcttaaaa
    14281 attctgatgc tatagagata aaaattggtc aaggaacaaa acctggtatg ggaggtcatc
    14341 ttcctggcga aaaagtaact gaagaaatag caaaagtaag aaataagcct gtaggtcaag
    14401 atgtaataag tccatcttgt tttgaggaaa tacaatccaa agaggattta aagaaactta
    14461 tagatgaact tagagaagtt tcagaagggc gtccaatagg tgttaaaatt tcagcaggtc
    14521 atattgaaaa agatatggaa tttatagctt atgctaaacc tgattttgta actattgatg
    14581 gtcgtggtgg agctactgga gcaagtccaa agttgcttaa agatgcaact tcaattccaa
    14641 caatatttgc tctttatcgt gctagaaaat acatagatac acatggatta gatatagatt
    14701 tggttataac aggtgggctt cgtatatcta ctgattttgc taaggcaatt gctatggggg
    14761 ctgatgcagt tgctattgca agttctgctc ttatggcagc ggcgtgtcaa caatatcgta
    14821 tatgtggttc tggtaagtgt cctgtaggag tagctactca ggatgaggaa ttgagaaaaa
    14881 gattacatat agaaaatagt gctaatagag tggcaaactt tttaaatgta agtttagaag
    14941 aacttaagac ttttgctaga atttctggtc ataaagatat acatgattta agtgttgatg
    15001 atttatatac tgttaactct gaaatatcaa attatacaaa catacagcat gtatagattg
    15061 aagtttgaaa ctattagatt ttagatagct ataaaagtaa atgataaata tattttataa
    15121 aagagagtgt cttaaaatga actttttagt tcataagtca ctcttttttg tatgaagcca
    15181 aatatattta tcattttagc aatgaaaaag aatcttatct ctttattttg agatagactc
    15241 cttctattta tgaaaattaa agatattaaa ttgtaaaata ttctgtgatg tcgtactatt
    15301 aaactaaaga ttctgtactt taaagtaaat aaaaatttaa ggagtgctta ttatggaaag
    15361 attgaagata caagttttag ttgacaataa tacttatatt gatagatact ttgttggaga
    15421 accagcagta agctattata ttgagattga tggaaatagg attttgtttg attcgggata
    15481 ttcagatgta ttcatatcaa acgctgaaaa attaaatata gatttaggaa atttaactca
    15541 tgttgttttt tctcatggac ataatgacca tacaagaggt atccagtttt tagaaaatag
    15601 gtatgattta tctacagttg aacttatttc acatcctaac tgttttatac ctagaaaaca
    15661 tggagagaaa agcataggag ctccattttc tgctgaggaa attaaaaata tttttatgta
    15721 taatcctaag gataagccat ttaatctaag taagaattgt gtgtttttag gtgaaatacc
    15781 ttctataaat aattttgaaa aaagagctaa aattggtaaa tgtaaaatag gagatttgtg
    15841 ggaagatgat tatgttttag atgattctgc tatagtttgt aaaactgata aaggtatttt
    15901 tattgttaca ggatgttctc atagtggaat ttgtaatata gtagagtatg ctaaaagggt
    15961 atgtggagat gatagagtta ttggtatact aggtggtttt catctttttg aattgaataa
    16021 taggttagat agtacaatac aatatttgga taaagaagaa ataagaatgt tgtacccttg
    16081 tcactgtgtt tcttttaagg taaaagctaa gatgaatgaa atattgaata taaatgaagt
    16141 tggagtaggg ctgaccataa gtatatagta tttgaaagta tataagattt aagttgaagg
    16201 ttaaaaagat attttctata taaagtatct tttttgttac gtttatttta agagaaatca
    16261 atatactttt atatgaaata ttgtaaaaaa atttattttt aatgagaata tattttcatt
    16321 aataaacaat tttattatgt attaataatg ctagaataa
//
LOCUS       DAMJWP010000471        16358 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.guided.073, whole genome shotgun sequence.
ACCESSION   DAMJWP010000471 DAMJWP010000000
VERSION     DAMJWP010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16358
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.guided.073"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(116..808)
                     /locus_tag="PC700_003815"
     CDS             complement(116..808)
                     /locus_tag="PC700_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDF2583021.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCKEGILRAVPYSSPIVEGFKKVDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESEATKLAILNATSKELQKIKYTYLKSIEELDKVSHGLIEECYDADLQFHKEIILAS
                     HNPIFIKYYELIPKTVSSNQYFGFKYRNSLKDMISHHNKIMMAFDSKDSYLGYTSMYN
                     HLEDVIQLFQHD"
     gene            complement(834..2021)
                     /locus_tag="PC700_003816"
     CDS             complement(834..2021)
                     /locus_tag="PC700_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2583022.1"
                     /translation="MKKISNQKLLLLSIVTFFWFSQYVYIPFQNPYLSACNVSTNMIG
                     IIIGAYGISQCLLRIPVGVFADSINKHKIFILSGIFFAGLASLIRVFSPGGNGFLIAN
                     ILSGISSSTWISYMVHYTNFFSTEHQQKATSTIILVNNLGMLIGFVMSTLFYEKLGMT
                     ALCIMSVLSSVIGTILALNIKNHNSKKIPEKISIKKYLSICKDKRLVFFSFMALIQQG
                     IQMSTTMSFTTQILKNLGSSSTLIGLSSISYMVCAVASSGFASTKFCTKYGPKFWIPL
                     VFILVSAYCFLVPTIKNINIIFVLQVLPGISTGILLSYLTSESMKNVPKHQKSTAMGF
                     FQAIYAIGMSVFPMIVGKISEYTSIQYGYFILAIIAILASILSYTYYKKVDLTLNYDI
                     IQS"
     gene            2357..4234
                     /locus_tag="PC700_003817"
     CDS             2357..4234
                     /locus_tag="PC700_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2583023.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKTEGVITVVESMGQIQVVIGNKVKTVYDEVIKIVPQSNDVGKKNESQEKQSIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYVEKVLEKYIPEVIKIIMVPTLSLVVMLPATLFLFGPIGIYIGNVI
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGAA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AFGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSFIKVETKDGVIDITSPVEGECIELSEVKDDVFASKAMGEGIAVLP
                     TKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKKAGYDVTTPVIVNNTFDFGQVVSCKSSYVSTNDNIISLVLAG
                     N"
     gene            4546..5952
                     /locus_tag="PC700_003818"
     CDS             4546..5952
                     /locus_tag="PC700_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDF2583024.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLDFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNTVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEVLDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            5980..6828
                     /locus_tag="PC700_003819"
     CDS             5980..6828
                     /locus_tag="PC700_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2583025.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            7489..8181
                     /locus_tag="PC700_003820"
     CDS             7489..8181
                     /locus_tag="PC700_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2583026.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            8178..9113
                     /locus_tag="PC700_003821"
     CDS             8178..9113
                     /locus_tag="PC700_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2583027.1"
                     /translation="MKMDEQTIILFLFLLSIGLLFLSIFAFSKLGHIHKRLKDIEEIL
                     ADVQIGNENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIGQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVDDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQISVESEIGKYTVFTVSLPLI"
     gene            9261..10183
                     /locus_tag="PC700_003822"
                     /pseudo
     CDS             9261..10183
                     /locus_tag="PC700_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            10167..10937
                     /locus_tag="PC700_003823"
     CDS             10167..10937
                     /locus_tag="PC700_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2583028.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVPKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVTVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPLLSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            10947..11729
                     /locus_tag="PC700_003824"
     CDS             10947..11729
                     /locus_tag="PC700_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2583029.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGIAVLLSLFSFLCTFILGLLFCNINISVTLIVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            12106..12642
                     /locus_tag="PC700_003825"
     CDS             12106..12642
                     /locus_tag="PC700_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HDF2583030.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            12674..13063
                     /locus_tag="PC700_003826"
     CDS             12674..13063
                     /locus_tag="PC700_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDF2583031.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            13100..13486
                     /locus_tag="PC700_003827"
     CDS             13100..13486
                     /locus_tag="PC700_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HDF2583032.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGSQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            13613..15055
                     /locus_tag="PC700_003828"
     CDS             13613..15055
                     /locus_tag="PC700_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDF2583033.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVENESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLIDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            15352..16167
                     /locus_tag="PC700_003829"
     CDS             15352..16167
                     /locus_tag="PC700_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2583034.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINNFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKRVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEIRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
BASE COUNT         5919 a         2026 c         2986 g         5427 t
ORIGIN      
        1 tgaaatgatg aaaatatatt tgatttcata caaaaaagag tgcctcataa ctaaaaagtt
       61 cactttgaga cactccattt ttatatgtta gttgcattta aggtaaaata gaagtttaat
      121 catgttgaaa taactgaatt acatcttcta aatgattgta cattgatgta taacctaagt
      181 aagagtcttt actatcaaat gccatcatta tcttgttatg gtggctaatc atatccttaa
      241 gagaatttct atatttaaaa ccgaagtatt ggttagaaga aactgtttta ggtattaact
      301 cataatattt tataaaaatt ggattatgag aagctaaaat tatctctttg tgaaattgta
      361 agtcagcatc ataacattct tctatcaaac catgagaaac cttatccaat tcctctattg
      421 actttaggta agtatatttt attttttgaa gttctttaga tgtagcattt aaaattgcta
      481 gtttagtagc ttcactttcg ataagttgtc ttgctcttaa agattctaca taaaattctt
      541 gtatgttatg atttttatag atttcaccat catcaaatag gtcaactttt ttaaatcctt
      601 caactatagg acttgagtaa ggcacagctc taagtattcc ttccttacag agtatattta
      661 tggcatttct tacaggtatt ctacttatgt taaataggtc agcaatcttt ctctcacttg
      721 gtaatttact attaatttta aaagttccat ttagaatgtt taatttaata taatcaataa
      781 ttttttcagt ggcatttaag cttttcataa aaactcctaa acatataatt tttttatgat
      841 tgtattatat catagttaag agttaaatct acttttttat aatatgtata agacaggata
      901 gaggccaaaa tagctataat agccaagata aagtatccat attgtattga agtatattcc
      961 gatattttac caactatcat tggaaataca gacattccta tagcataaat tgcttgaaaa
     1021 aaacccattg cagtagattt ctgatgtttt ggtacatttt tcatagactc gctagtaagg
     1081 taagaaagga gtatacctgt agatatacct ggtagcactt gtagtacaaa tattatatta
     1141 atatttttta ttgttggaac taaaaagcaa taggctgaaa ctaaaataaa aactaaagga
     1201 atccaaaatt ttggcccata cttagtacaa aatttagtag aagcaaagcc agaagaagca
     1261 acagcacaca ccatgtaaga tatagaagat agacctatga gggtagaact agaacctaag
     1321 tttttaagaa tttgagtagt aaatgacatt gttgtagaca tttgaatacc ctgttgaatt
     1381 aatgccataa aggaaaaaaa tacaagtctt ttatctttgc agatagataa atatttttta
     1441 attgatattt tttcaggtat tttttttgaa ttatgatttt ttatatttaa ggctagaatc
     1501 gttccaataa ctgaagacaa tacactcatg atacataagg ctgtcattcc taatttttca
     1561 taaaataaag tactcataac aaatccaatc aacattccta gattgtttac caatattatc
     1621 gtactcgtag ctttttgttg atgttctgta gaaaaaaagt tggtatagtg aaccatgtaa
     1681 gaaatccaag ttgatgatga tattccagat aaaatgttag ctataagaaa accattgcct
     1741 ccaggagaaa agactcttat aagagatgct aaaccagcaa agaaaattcc agacagtata
     1801 aatattttat gtttatttat agaatcagca aatactccaa caggaatcct taataaacat
     1861 tgtgagattc catatgcacc tattattata ccaatcatat ttgtagatac attacatgca
     1921 cttaaatatg gattttggaa aggtatatat acatattgtg aaaaccagaa aaatgtaaca
     1981 atagaaagta aaagtaattt ttgattagat atttttttca tattatcctc catatggtat
     2041 ttttataatg caaataatat accatttaga ttttttaatc aagtactttc tctattaaaa
     2101 aaaatttaaa tatataatta ttatttacaa aaaaattata ctttatagtt gaattaagaa
     2161 aacgcttatg ataatataaa gttaacaaat ttgaatatat aacagtagac gtgttactgg
     2221 taatgcaggc aagatcgaat caatttttat agattatcta tagagtacta tgggtataat
     2281 ctatttattt ggttcgattt ttttgtttaa ttggccataa aacaaaaagc ctatagttga
     2341 aaatggagga ttttttatgg attataaaaa tatagctcaa gaaattctac taaatgtagg
     2401 aggaaaagag aatgtaaatg aagtgactca ttgtatgaca agacttagat ttaaagtcaa
     2461 aagcgcttct aaagtgaata aggataaatt atctaaaact gaaggagtaa taactgttgt
     2521 tgagagtatg ggtcaaatac aagttgtaat tggaaataaa gtaaaaacag tatatgatga
     2581 ggttataaaa attgtacctc aatcaaatga cgtaggtaaa aagaatgaaa gtcaagaaaa
     2641 gcaaagtata attaattcta tattatcagc agtagctggt atatttacac ctacaatacc
     2701 tgctatagca ggggttggta tgattaaagg tattttatca gttcttgcta tgtattatat
     2761 gaataaaaat ggagtagata taaaagaaac gcaaagttat attatcttaa atgccatggc
     2821 cgattctata ttctatttta tgcctattat actaggatat actgcggcta aagtatttaa
     2881 tgcaaataaa ataatatcta tggtattagg agctacactt tgttatccaa cttttactgc
     2941 tttgatggca ggtgaagaat ctgttagatt tttaggattg gcagtaacaa aagctacgta
     3001 tacatcatct gttattccaa taattattgc tatatgggct ctttcatacg ttgaaaaagt
     3061 attagaaaag tatatacctg aggtaattaa aataataatg gttccaactt tatcattggt
     3121 tgtaatgtta cctgcaacat tatttttatt tggaccaatc ggaatttata taggaaatgt
     3181 cataaacttt tcttataagt atatttatga attaagccca gcgttgtgtg gagcatttgt
     3241 aggtgggtta tggtgtgtgt tggttatatt tggtgctcat agagcattat taccaatagg
     3301 tataagtgat gttgctcaaa caggaagaca gaatttattg gcatttgcag gagctgcaaa
     3361 cttttcacaa gcaggggctg ctctaggagt attctttaaa actaaaaatc aaggactaaa
     3421 aacaatttct atgtctgcaa ctataacagc tttatttggt ataactgaac ctgcaatata
     3481 cggtgccaat cttagattaa aaaaacctat ggtatgtgcc gtaatatgtg gtgcatttgg
     3541 aggtgcgata atgggaatgg gtggagctta tggtaatgca tttgcaaacc aaggtgtttt
     3601 aactatacct gtatatgctg aagcaggtgc attaggtttc ttaagttatc taggtggttg
     3661 tgctatagca ttttttggtt cagctatttc aacttatcta gttggatttg aagatttgga
     3721 agagtcttct aataaggaaa attcatttat aaaagtagaa acgaaggatg gtgttattga
     3781 tataacttca cctgtagagg gcgaatgtat agaattgtca gaagtaaagg atgatgtatt
     3841 tgcttcaaaa gctatgggag aaggtatagc tgtgttacct acaaaaggag taataacagc
     3901 ccctactgat tgtgaagtag cttctttatt tccaacatta catgcaatag gattaaaatt
     3961 agataatgga gcagaaatgt taatacatgt tggaatcaat acagtagagt taaatggtaa
     4021 atactttaca aaacatgtta atcaaggaga tttagtgaaa aaaggtgata aacttatatc
     4081 gtttgatata gataaaataa aaaaggctgg ttatgatgta actacgccag ttatagtaaa
     4141 caatacattt gattttgggc aagtagtttc ttgtaaatct agttatgttt caaccaatga
     4201 taatataatt tcattagtat tagctggtaa ttaagttaat ttgactagag ataagtaaat
     4261 tgatttaaaa ggaaataagt aaatcaacaa ttaaatacat ctaaaagtaa gtttattgca
     4321 atgaattgtt aaagaaaata attaatatgg agtaaagcta ttgcaatgaa gtaacaaaga
     4381 aaataattaa tatagagtaa gtttattgta atgaattgtt aaagaaaata attaatatag
     4441 ggtaaatcta tctcaatgaa ataacaaaga aaagagttaa tatagagtga acttattgca
     4501 atgaatcaac aaaaaaaata atcaatataa aaaggagaaa tatttatgaa tacaggattt
     4561 ccaaaagact ttttatgggg agcatcatct tctgcatttc aagtggaagg agcttgggat
     4621 aaagacaata aaggaaaaac cgtagccgac tataattctt ttaaaaagtc tcatttacag
     4681 gcagatacta aagttgcatc agacttttac cataattatg aagaagatat tgaattaatg
     4741 aaagaattag ggatgaaaac atatcgtttt tcaatttcat gggcaaggat aataccagat
     4801 ggtgaaggag agataaacca gaaaggttta gacttttata ataagataat agacaaacta
     4861 atagagtgtg atattgagcc attcgtaact ttataccatt ttgaccttcc atttaaatta
     4921 gtagagaaat ataatggttg ggaaagtaga gaaactgtct atgcttttga aagatttgct
     4981 aaaatatgct ttaagcattt tggagatagg gtaaagtatt ggcaacctca taatgaacag
     5041 aatttaattg ttagagtgga ggagagaatt aatatatatg atgaaacaga ttcatggaag
     5101 atagataaaa tgcgtgctca gatggattat aatctttgct tagcacatgc attagcagtc
     5161 aatgcatgtc atgagatgat aaaagaaagt aaaattggag cagcagtatc atcatctgta
     5221 acatatcctc ttacttctaa gccagaagat gtatatgcag ctagaatgaa tgataatttt
     5281 aaagtgtact atatgcttga tatgcatcat tatggagaat atccagggta ttatatgaag
     5341 tatcttgaaa aaagaaatac agttcctcac atggaagatg gggataaaga gatacttaaa
     5401 aaagctaaaa tggattttat agctgtaaat tattatagga caaattgtgc agaagcttta
     5461 cctgaagatt cacaacatcc atttggatta agagaaggaa ctgttgattt tagtatgtat
     5521 ggactattta aaatgtcaat gaatcctaac ttagaggctt ctgagtatgg agcagcaata
     5581 gacccatcag gtcttagagt tgccttaaat gaatattggc aaagatatca cttacctgtt
     5641 ataataacag aaaatggtct aggagctaaa gatatattag aagatggaaa aattcatgat
     5701 gactatcgta tagactattt aagaagtcat ataaatgcat gtaaactagc tattgaagat
     5761 ggtgtagaga tgattggata ttgtccatgg tcatttacag accttttaag ttcttctcaa
     5821 ggttttaata agcgttatgg acttgtttat ataaatagaa ctgaccatga agttttagat
     5881 ttaaaaagaa tcaagaaaga tagtttttac tggtataaag aagttataga gaacaatgga
     5941 atagtaaaat aaacataaat atatattgag ggttttagta tgtacattac aaaggtatta
     6001 aataattcgt tgctacttgc aaaggatgat aatgatgaag aaataatatt gatggggaaa
     6061 ggcattggcc ataattataa ggccgggtct gagttaaata aaaaagatat agaaaagatt
     6121 tttgttcttc atgatgaaaa tataaaaaag agtattatac aattggcaac agaaatagat
     6181 gcagaatatt ttggtatagc tcaaatgata attgcatatg gaatagaaaa atataactta
     6241 aaacttatga accatatata tttggcctta actgaccaca ttgcctttgc agtgagacgc
     6301 tttaaagaag gtataatgat tgaaaatcac tatttgttcg aaatcaagga atttaatcca
     6361 aaagagtatg atataggaaa atatgctatt actgttttta aggaggttct tggtttagac
     6421 ctaccagaag aagagattgg taatatagca ggccatttta taaatgcaca acaagataat
     6481 ccttatagtg atagaaataa gagaagtgct aaaattgtaa atgctatttt gcaaatagtt
     6541 cattatcact tttctattgt atataataaa gagtcatttt attataggag atttgtaatg
     6601 catctaaagg ccttttcaca aagatttctg gctaatgaac catcaagaga aaaaatagat
     6661 tttatatatg agcaagttca aaaaaattgt aaggaagagt atgaatgtgt gaaaaaggta
     6721 ggagcttata tgtataagga atactccaga gaattaccaa gacaagagga gttatatttg
     6781 atgattcata ttcataagat tttaggagaa ttatgcgaaa atagttagaa tatcttaata
     6841 tgctagtaat attataaaaa attaaaaatt aactacactt taaatatgaa caattgcatt
     6901 acttaagggg gttgtcttaa aattgatttt tttaattaaa tttgaagctc aactcctttt
     6961 ttaaaccttt atatcattac aactttaatg tttatacgcc tgtactttag atgtatatct
     7021 actttttaca tttctcaaca tgcaaaccta ccattttttg cagtatgtta attgaaataa
     7081 accattgagt taaccctaaa tttattctgg acttatagga atagttccaa agatacttta
     7141 aaatattttc tggaataatt gcttatttca catatgtttt attgtgatgt tttgatttaa
     7201 taaattcttc aactctgtat atagtaaatg tattatcatg tttgttttat tcaagcacac
     7261 ttataatacg cttgattttt tcacaatgat gaaatatact aatgggtgtt ataggtataa
     7321 gaatcgaatt acacttgaag aagtaagtga taaatgagat ataaatcctc atatgataaa
     7381 ctactatttc attgaggaac atattctaag tgatgtcaag atactacaat atgactaaat
     7441 tcctagtgat aaaaaagtct acgaatgagt gtagaaaggg gaactaagat gaataaagtt
     7501 ttaattatag atgatgataa agatctttgt attttgttaa aaaagagtat ttcaattgaa
     7561 cagattgaag caaactattg ccattctggg agagatggac tggttgaact ttcaaaacaa
     7621 gattatcagt tagtagttct agacgtgatg atgccaggta tggatggttt tgaaactatg
     7681 gaacaaatac gaaaatatag cagtattccc atcttaatgc tgacatcaaa aagcgataat
     7741 tacagcaaag tgcatggatt gcgagctggt gcagatgact atctaaccaa accatttgaa
     7801 attacagaat ttactgctcg tgtactgtct ttaatccgac gttacacccg ttttaatcca
     7861 aaaaatgagg aaccacagca actttctttc aaaggtttgt tgattgacct tgaccagcat
     7921 acagtgacta ccaatggcga acctatgttg cttccaccca aggaatttgg tgtgcttctt
     7981 tactgtgcaa ggcatcaggg taagattttg acaaaacagc agatttatga agctgtgtgg
     8041 ggtgagccat atgtatatga tgacagcaat atcatggcta ttatcagtcg cctaagaaaa
     8101 aaaattgaac tagacccatc atctcccatc tatttgcaaa caataaaagg aattgggtat
     8161 caattcaaca cggaggtatg aaaatggatg aacaaacgat aattcttttc ttattcttgc
     8221 tgtcgattgg tctacttttt ctttccatat ttgctttttc caagcttggg catattcaca
     8281 aacgcctaaa agatatagag gagatactgg ctgatgtaca aattggaaac gaaaatcgca
     8341 aaatactaat taagccatgt gatgttatgg ctccattggt ttatcagctt aatgagattg
     8401 tctacgatta tgaaaataag ttactttctc tgaaaaagtc ggataaagct agcaaacaac
     8461 ttatgacaag tctatctcat gatgttcgta caccattaac aactctgatt ggttatctgg
     8521 atgctgtaca cagtggtata gttatcggac aagaacggga ggaatacctt gaaatcgcta
     8581 ggcgtagagc ctacgacctc aaggattaca ttgatgttct ttttgactgg tttcggttga
     8641 actctgatga atttacgctg tcaatagaat ctgtggaaat tgcagagttg tcacgcaata
     8701 ttttgaaaga ctggattcct atattccatg aaaagaaact gaattttgaa attgatattc
     8761 cagaaaatcg actaatggta aatctcgacc cagatggata ttcccgtgtg gttaataatc
     8821 tggttcaaaa tgttcttgct cacagtaagg ctcggcaaat caagattacc atgagtgagg
     8881 attccagaat ggtattactc cgtgttgatg atgatggtgt tgggattgcc agagaaaatt
     8941 taccacatat ttttgagcga ctgtataagt gtgataaagg acgttcagaa aaaggaagtg
     9001 gtcttggtct atctattgtc agtcaaatgg tggagcgaat gggtggtcag atttccgtag
     9061 aaagtgagat tggaaagtat accgtattta cagtgagctt accattaata taaaattgtt
     9121 tttcccccat gtgacatgta tacacagggg gattttttgt aagttataaa gttgcaaggt
     9181 taatacaagg ttgttgcaag gttaatgtaa gattgaggtg gtatgatagc tttaataaac
     9241 aaaggaggat tttattattt atgaataatt taattattga aacaaaaaat ctcactaagc
     9301 aatatggtgc tcaaaagagc gtttctaatt tgaatattca cgttcagaaa ggtcgcattt
     9361 acggattgct aggaagaaac ggtgcgggta agacgacaac tatgaaaatg ttattgggtc
     9421 taacaaaacc aactactgga gaaattaaaa tatttggtca ggacattcac aataacgaga
     9481 agaagcttct tcctcgaatt ggatgtttga ttgaatcacc agggttttat cctaatctga
     9541 caggaacaga aaatctcaaa atctttgcaa ggcttagagg aataccaaaa aaaatgcagt
     9601 agaatcagca ctggatgtag tcggtttgcc aaacaatgac aaaaagttat tttctcaata
     9661 ctctcttggt atgaaacaga ggttggcgat tgctcttgcc attatgcatg acccacaact
     9721 gttgattttg gacgagccta tcaatggact tgatcctatt ggtattgcag aagtaagaaa
     9781 gtttattcgt gatctctgca cagaacgagg taaaactatt ttgatttcca gtcacattct
     9841 ttctgaaatt gccttgttgg cagatgacat tggtattatt gaccatggta ttttgttgga
     9901 ggaagaaagt cttgcccaat tagaagaaaa aaacagtaaa tacatacatt ttgctgtatc
     9961 tgatgcagca catgcagcaa ggcttctgga aacccaactt aacctgaaaa atttcaaagt
    10021 agcagataat tacaatatta tgctatatga tacagatgtt tctgttgcag aaataaatcg
    10081 tgctttcatt ctaaacaaca ttgaggtatc agaatctcat ttatgcgaag atactcttga
    10141 agattacttc aagaaagtaa cagggggtga aggaattgct tgatataatt tcatgtgaat
    10201 tttcaaaact caagcggtta aaatttatac tgatttctat tttaggtgct tgcttatttc
    10261 caattcccgc aacaattttg attgcaaagg acaacttgcc ctttgagcaa ttgttcaaat
    10321 tagttgtcaa ttttggatat tttttgctat taccaattgt attaagtatt gttgctagcc
    10381 aactattttt tatagaaaga gataacgatg tattaaaaaa tcttgcaaca gtgccagtgc
    10441 caaaaggaaa actggctctg gcaaaattgg ctgtgttgct gtttatatca ttgttctact
    10501 ccgttgcagg gttaggagct actatcattg gaggtttcat tgttggaata gtagaggggg
    10561 ttactgtaaa attaggaatg agcattgctt ttggaattat gttttttgtt actgtacttc
    10621 ctgttgtggt actcattgta tatttcaatc gcagctatat tttttctatt atactttctt
    10681 ttgtatatgc tattttcaat tttagtatat ccttaaatat tatcaatttt gaaccaaata
    10741 acccactatt aagcgtatta cctgctcctg ttataatgcg ttggtggatg gcattttggg
    10801 gagatcccac tggcgaatac acggcactta gacagccata tttgctctca actcctgctt
    10861 gtgtaggtat tttatgtttg ataaccatta tagcagtcct gctgatttgt atgatataca
    10921 aaaagcagga gaattaggag gtatctatgc ttgatataat tactacagaa tttcaaaaaa
    10981 taaaaagata taatattcta tggattggtg ttgttgctgt tctgttttca gcactattgg
    11041 cagtttttca ggttgtaagt tcacacggca gtgaccctct aacgtatgag agctttgcaa
    11101 acggagttat ctggaataat ttcagtctta gttttccatt tgggattaca cttattggag
    11161 gatatcttat taaccgtgaa tatacagacc aaaccttaaa aaacatccta actgttccta
    11221 tttcattacg caaattgcta gtgggaaaat taattgcggt tggaggaatt gctgtattgt
    11281 taagtctttt cagcttctta tgcacattta ttttggggtt gttattttgc aatatcaata
    11341 tatctgttac actcattgta aaatccttag ttcagatttt gtctgttaat ctgtattgtt
    11401 atgttgctgt tttgccgatt attgcttatt ttggtcgtaa acaaaatgca tttctgacgg
    11461 gtgtagggat tgcatttgta tacgggtttt gtggtgtgtt tgttgctgga agaaatctga
    11521 cagactttta tccaatcaca gcaggtcttg gtctagttgg atacaacaat ggtgctggta
    11581 ctgtttatca gccacttatc ggtgctatga ctttgattgt aattctaatc ttgacaacta
    11641 ttctacttgt atttacacct aattatgata aagttatgac catatctaag aaaaaaggga
    11701 taaagcataa aaagtattct cataaataac ataaatattg tttctattat ttaagttgct
    11761 agacagtgaa aagaaaaaat ctgtcgtaca gtggctatat gctcatgtat tcaagaagct
    11821 acagttttga tttttcataa ctgtagcttc tttttgtatt tattaaatca cggctatcat
    11881 aatacttgca ataggtatag cgacaagaga gagtactgtc atgtgtgttt tatatattac
    11941 attaagtata ttctaaaata tatgttttaa ataatatatt tgaaaatagg aatatttaaa
    12001 taaaaaaaat aatattatat atagttgaca aaaattggca aatgatataa tatataaata
    12061 tagaaatagt aatcattact attattaaaa agggaggaat taattatgaa cttaaaagga
    12121 actaaaacag aaaagaattt aatggcagga tttgcaggag agtcagaagc aagaaataaa
    12181 tatacatact acgcttcaaa agcaagaaaa gaaggttata atcaaatagc tgctatattt
    12241 gaagaaactg ctaacaatga aaaggaacat gctaaattat ggtttaaact tttacatgat
    12301 ggtatgcctt caacagagga aaatttaaag gatgcagcag caggtgaaaa ctatgaatgg
    12361 actgatatgt atgcaaaatt tgctaaagaa gctagagaag aaggatttga caagatagct
    12421 tatttatttg aagctgttgg aaaaatagag aaagagcatg aagaaagata cttaaagctt
    12481 ttagaaaact taaatgaagg aaaaatattt aaaagagatg aagaagttgt ttggcaatgt
    12541 caaaactgtg gtcatgtgta tgtaggtact gaagcaccag aaaaatgccc agtatgtgac
    12601 catccaaaag cgtatttcaa tataaaagct gaaaattatt aaaaattatc attattaaaa
    12661 aggaatgtta actatgaaat tttctaaaca acgagaactg attttaaatg aaatattaaa
    12721 taatccggtt catcctactg cggattacct atatgaaaac ttaaaaaaag ataatccaaa
    12781 tttaagctta ggaactgttt atagaaattt agctcagcta acggaacatg gctttataag
    12841 aaaggttagt attccagggt atccagatag atttgatggt cgaatagata atcactatca
    12901 tatcatatgt gaagtatgtg gagaggtata cgatttagaa tccgaagttc ttaataattt
    12961 acaagaatta atatctgagg agacagacat aaaaataaca tcttataaca taagttttaa
    13021 agggatttgt aataattgta aaaggtgtag ccaagtaggt tagttttcaa attctaaaaa
    13081 taattaatga ggtgagttta tgtgtagtga acaaaaattt tttatatgtg agacttgtgg
    13141 aaaccttgta ggaatgatac aaagtggtgg tgtacctatt ttctgttgtg gaaaacctat
    13201 gaaggaactt gttcctaata caacagatgc agcagttgaa aaacatgtac ctgtaattga
    13261 agtggatgga aataatgtta ctgttaaagt aagtagcaca actcatccaa tgactaaaga
    13321 acatcatata gcatgggtgt atcttatgac tgaacaaggt agtcaacgca aatgtctagc
    13381 agtagatgga gaacctgtag ttaaatttgc tttgaatgat gatgataaag tgatttctgc
    13441 ctatgcttac tgtaatttac atggattatg gaaggctgaa ctgtaatatt taaataaatt
    13501 gagtcaaaga ggaggctata aatgtatttt catacaaata gcctcctata aatttatata
    13561 aattcaagtt ttaattttgt ttaatgtcaa tataatataa ggagaaaata atatgtcaat
    13621 ttataaatgt agtgtttgtg gatatattta tgatgaatct aagaatgata aaacatggga
    13681 tgaattaagt gaagattggg aatgccctgt atgtacaaaa ggtcgtagtt attttggaaa
    13741 gataagcact gtttactatg aagaagatga aaagatagca gaagacatag ttgaaaatga
    13801 atctaaatta aatactgaaa aagagggaga tttaaattac ttaagtacat atttaagaag
    13861 agatgatgaa gttgagaaac atatggatat aattcacgaa atggctgtta caggaaaatc
    13921 tataatagaa cctatgagaa ctaaactacc agtaatttct tgggatgata ttttaattat
    13981 gggtgctcaa ttaaatccat tacctttaaa tgagcatgat gaagtaaata caacaacaat
    14041 aataggaaaa aaagcaaaga aacctatgat tatagaaaat ccagtttata tttcccacat
    14101 gtcatttgga gctttgtcta aagaactaaa aatagcttta gcaaagggtg cagcccaaaa
    14161 taagacagct atgtgtagtg gtgaaggagg tatattacca gaagagaagg aagcttcata
    14221 caagtatatt tttgagtatg taccaaataa atatagtgta actgaagaaa atcttaaaaa
    14281 ttctgatgct atagagataa aaattggtca aggaacaaaa cctggtatgg gaggtcatct
    14341 tcctggcgaa aaagtaactg aagaaatagc aaaagtaaga aataagcctg taggtcaaga
    14401 tgtaataagt ccatcttgtt ttgaggaaat acaatccaaa gaggatttaa agaaacttat
    14461 agatgaactt agagaagttt cagaagggcg tccaataggt gttaaaattt cagcaggtca
    14521 tattgaaaaa gatatggaat ttatagctta tgctaaacct gattttgtaa ctattgatgg
    14581 tcgtggtgga gctactggag caagtccaaa gttgcttaaa gatgcaactt caattccaac
    14641 aatatttgct ctttatcgtg ctagaaaata catagataca catggattag atatagattt
    14701 ggttataaca ggtgggcttc gtatatctac tgattttgct aaggcaattg ctatgggggc
    14761 tgatgcagtt gctattgcaa gttctgctct tatggcagcg gcgtgtcaac aatatcgtat
    14821 atgtggttct ggtaagtgtc ctgtaggagt agctactcag gatgaggaat tgagaaaaag
    14881 attacatata gaaaatagtg ctaatagagt ggcaaacttt ttaaatgtaa gtttagaaga
    14941 acttaagact tttgctagaa tttctggtca taaagatata catgatttaa gtgttgatga
    15001 tttatatact gttaactctg aaatatcaaa ttatacaaac atacagcatg tatagattga
    15061 agtttgaaac tattagattt tagatagcta taaaagtaaa tgataaatat attttataaa
    15121 agagagtgtc ttaaaatgaa ctttttagtt cataagtcac tcttttttgt atgaagccaa
    15181 atatatttat cattttagca atgaaaaaga atcttatctc tttattttga gatagactcc
    15241 ttctatttat gaaaattaaa gatattaaat tgtaaaatat tctgtgatgt cgtactatta
    15301 aactaaagat tctgtacttt aaagtaaata aaaatttaag gagtgcttat tatggaaaga
    15361 ttgaagatac aagttttagt tgacaataat acttatattg atagatactt tgttggagaa
    15421 ccagcagtaa gctattatat tgagattgat ggaaatagga ttttgtttga ttcgggatat
    15481 tcagatgtat tcatatcaaa cgctgaaaaa ttaaatatag atttaggaaa tttaactcat
    15541 gttgtttttt ctcatggaca taatgaccat acaagaggta tccagttttt agaaaatagg
    15601 tatgatttat ctacagttga acttatttca catcctaact gttttatacc tagaaaacat
    15661 ggagagaaaa gcataggagc tccattttct gctgaggaaa ttaaaaatat ttttatgtat
    15721 aatcctaagg ataagccatt taatctaagt aagaattgtg tgtttttagg tgaaatacct
    15781 tctataaata attttgaaaa aagagctaaa attggtaaat gtaaaatagg agatttgtgg
    15841 gaagatgatt atgttttaga tgattctgct atagtttgta aaactgataa aggtattttt
    15901 attgttacag gatgttctca tagtggaatt tgtaatatag tagagtatgc taaaagggta
    15961 tgtggagatg atagagttat tggtatacta ggtggttttc atctttttga attgaataat
    16021 aggttagata gtacaataca atatttggat aaagaagaaa taagaatgtt gtacccttgt
    16081 cactgtgttt cttttaaggt aaaagctaag atgaatgaaa tattgaatat aaatgaagtt
    16141 ggagtagggc tgaccataag tatatagtat ttgaaagtat ataagattta agttgaaggt
    16201 taaaaagata ttttctatat aaagtatctt ttttgttacg tttattttaa gagaaatcaa
    16261 tatactttta tatgaaatat tgtaaaaaaa tttattttta atgagaatat attttcatta
    16321 ataaacaatt ttattatgta ttaataatgc tagaataa
//
LOCUS       DAMJWP010000472        11371 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.guided.112, whole genome shotgun sequence.
ACCESSION   DAMJWP010000472 DAMJWP010000000
VERSION     DAMJWP010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11371
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.guided.112"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            144..842
                     /gene="vanR"
                     /locus_tag="PC700_003830"
     CDS             144..842
                     /gene="vanR"
                     /locus_tag="PC700_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanR-ABDEGLN family response regulator
                     transcription factor"
                     /protein_id="HDF2583035.1"
                     /translation="MSGKILVVDDERDIADLIEVYLTNENYIVYKFYSGLDALNCINL
                     NEFDLAILDVMLPGVNGFSLCQKIREKYTYPIIMLTAKDEETDKITGLTLGADDYVTK
                     PFRPLELVARVKAQIRRYKKYSGSLQSGEELSHIFSFLGLELNTQTYECHLNGKALSL
                     TPTEFSILHVLLKNKGSVVNSENLFHAIWKDEYYNKSNNTITVHIRHLREKLGDTVDE
                     AKYIKTIWGVGYKI"
     gene            878..1993
                     /locus_tag="PC700_003831"
     CDS             878..1993
                     /locus_tag="PC700_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2583036.1"
                     /translation="MKKINNFTYRKLRDKYKFIKREKNTEAYLKYKLYIMLAIYTGIV
                     LVLMFMADYTFSNFSNPLVKWLKLRRDVLFFLVFIIGYITIFNFYWKKPWNYLREIIN
                     ATHTIYQQNDELVELSKPLKEIEQQMNQIKMSVLLSQQAVKEAENKKNNLVMYLAHDI
                     RTPLTSVIGYLSLLKEIPNMDNSQKSKYTEVALDKAQYLEKLINELFEITRYNSNQIK
                     INFEKVDLYYMLLQLKDEFYPLLSEKGNRAILKANESLTIKADSEKLARVFNNILKNA
                     IAYSYPNTEIIISAVHKDSKVDIAFRNYGQTIPEEQLSAIFEKFNRLDEARKSDMGGA
                     GLGLSIAKEIINLHGGDITVQSKDEVTTFTISLPTSI"
     gene            2138..3025
                     /locus_tag="PC700_003832"
     CDS             2138..3025
                     /locus_tag="PC700_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2583037.1"
                     /translation="MELKIEHVTKKFKDKIAVEDVCLTLTPGVWGFLGANGAGKTTLM
                     RIITDIMKPTNGKILYNGKNIYIMGAEYRNLLGFLPQEFGFPQDFSVKDYLEYIAALK
                     DVPPREVKGKINNLLEILTLSDVKNKKIIKLSGGMKRRVGIAQAMLNDPKILIMDEPT
                     AGLDPGERVRFRNFISEFSHNRVVLISTHIVSDIEYISTQNAIMKSGKILDVGTTDKL
                     VKKVEGKVWECLISNRDISKYEMNLRIINQRSEKNNQVSIRYLAEKSQIDNSIAVEPR
                     LEDLYLWLFPEAVYSKEGK"
     gene            3026..4261
                     /locus_tag="PC700_003833"
     CDS             3026..4261
                     /locus_tag="PC700_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2583038.1"
                     /translation="MRIFYLEIKRVLHSRRTLILLLLALVLSALMAFLPITYEGINYL
                     DNNGKVVELSGLDAIKYKKTIYANNSEISSGVVTPKKLKHALNIWKKTEEKYGKVGSE
                     DFPLDIYSKSILPIRPLLNRLPEAFANSETGFATELSEINTNDLDSFYEQCKTHLNDI
                     MKIERQTSSAQMKAQELYRKVDTPFEIYPGYSKDAFDYIIFYIFILLIICVAITAPTF
                     SGEYQTQSDSILRCTKHGHTRLAFTKILALFSIFIVMFVVCISIHLAISDLAFGTECL
                     KTSFQMLFSVISLVNINLLQAQILIAIGGLISILAMVSLTLFLSAKCKDSLTSMLIAF
                     SICLIPMGIGVSWIGYLLPPGGIGLKISLLYQMTSFNFLHLGGMSFWTPHIILFFSVI
                     EIPIFIFLAIRTYCKHQVS"
     gene            4643..5560
                     /gene="pstC"
                     /locus_tag="PC700_003834"
     CDS             4643..5560
                     /gene="pstC"
                     /locus_tag="PC700_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDF2583039.1"
                     /translation="MIVKSRLVGLEKNDNNGNKTKYVIEKVAKNIFLLSALVAVASLL
                     LIIGFVFYKGLRPFIFEGYSFIDFLTGADWVPSANKFGISSMIVASIFATLGALVIGV
                     PVGILTSVFIAEVAPKRLAKIMSPAVELLAGIPSVLYGVFGLAVIVPTIQSIFNLPKG
                     QSLLAIVIVLAVMMLPTIISVSETAIRAVPKAYKEGSLALGASKIETIFKVVLPAAKS
                     GVLAAVVLGVGRALGETMAVILVAGNSPVMPTAITDSVRPLTTNIALEMGYAFGTHQE
                     MLFATGVVLFTFILILNLVLNKLSNKAVN"
     gene            5562..6386
                     /gene="pstA"
                     /locus_tag="PC700_003835"
     CDS             5562..6386
                     /gene="pstA"
                     /locus_tag="PC700_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDF2583040.1"
                     /translation="MRKFKENLLKSLVYLSALFTIVSLVIIVGFIFIKGIGNMNFNFL
                     FSNYSASGDGGILPMIITTLYTVVVSIVVATPIGILAAIYLQEYAKKGKAVTAIRFAT
                     ESLSGIPSIVYGLFGGIFFVVTLKMGYSIVAGSLTVAIIILPVIIRTTEEALKTVPQA
                     YRESSLALGATKFQTLYKVVLPSAIPGILSGVILSVGRIIGESAAILLTAGTVAKMPG
                     GIFDSARTLTVHSYLLTKESGDIATAASIGIVLIVIVLALNMLARFVAKKLNKANY"
     gene            6405..7169
                     /gene="pstB"
                     /locus_tag="PC700_003836"
     CDS             6405..7169
                     /gene="pstB"
                     /locus_tag="PC700_003836"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDF2583041.1"
                     /translation="MELIDKIKMSVKDLDLFYGDKQALKKINMDIKENKVTALIGPSG
                     CGKSTFIRTLNRMNDLIEDVTIKGNISVDGEDIYTSDDVINLRTKVGMVFQKPNPFPM
                     SIYDNVAYGPRTHGLRDKKQLDKIVEESLKGAAIWDEVKDRLKSSALGLSGGQQQRIC
                     IARAIAMRPEVILMDEPTSALDPISTLKVEELIEDLKKDYTIVIVTHNMQQAARISDE
                     TAFFLNGEVIEFSDTKTMFTTPVDKRTEDYITGRFG"
     gene            7197..7850
                     /gene="phoU"
                     /locus_tag="PC700_003837"
     CDS             7197..7850
                     /gene="phoU"
                     /locus_tag="PC700_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDF2583042.1"
                     /translation="MVNNSLDISIDTLKEQTVLMMEKCEEAIEKSVTCMIEKDLEGAR
                     KVVEDDEEINKLREEIRDKSIELIVLKQPMARDLRFIYSLGNISIELERIGDYAANIA
                     REMLKIGGEAYIKELIDIPKMAKECKSMMSQTREALILFDASIAYDVGTKDDIVDGLY
                     EQVQIDCLKIMHENSNTIDQGVKLLLIGRYLERIGDHITNVCEKIIFAIEGKMVEIG"
     gene            8008..8877
                     /locus_tag="PC700_003838"
     CDS             8008..8877
                     /locus_tag="PC700_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDF2583043.1"
                     /translation="MKLQKMGMRTIKTGISVTLCMILGNYLVENMFYSLVACIISVQD
                     TVKGSLKTGINRVEGTILGGIIGFIFAIIKAGDPFLCGLGVMCSIYLCNLFKIKSVVV
                     ACVTFLAIHLGVGNSDPVYYSLHRVLDTSVGVLFGVLVNYYVARPNYLNSVIDEFKKI
                     EELSMLLIEDKILRKENLNVEKFEEEVEKLEEIYSKLIDELDYSLSETNIEKIENALR
                     ICREIHFHMQSIELLENKLYLKEHTYKYLKKTYNVDKLDWNIDEEKSPVFNYHLMKIM
                     IEIELLRKLNKSY"
     gene            8964..10400
                     /locus_tag="PC700_003839"
     CDS             8964..10400
                     /locus_tag="PC700_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase"
                     /protein_id="HDF2583044.1"
                     /translation="MNTRNDNSKILEIYKMKLFGELVKAVWNDDIEDIENLPNKILNE
                     DLKDNLNNYFSGKFDKDTMVNLIRVTMGLDVTEDTDKNLKESLHEAVNLDEVKRPIIS
                     VISDACKYCDENNKEDESCKIRSKHINCNEEDVCSACGDCVSRCKLGAISDKIEFIPM
                     IKLLKDIKSPVYAIVAPAFVGQFGKDVSPGKLRTALKKIGFEDMIEVALAADMLTAKE
                     AYDYYEHMKKDEEGCFITSCCCPIWVSLIQNSFPQILENVSPSVSPMIACGRAVKILN
                     PDAKVVFIGPCMAKKKEAMLEDIKDAVDFVLTFKELQEIFDALELNPAIMDESNRVEA
                     SLSGRVYARTGGVSKAVKLSVRRIDKNIKFEEKAFEGTKECMDGLKKVLNKEIEATFI
                     EGMGCVGGCVGGPKRILSVEEGTKNVDEYSESTNMETPFDNLNVLQFLTSLGIKRVES
                     LGEKEEEKVKKIFSRDIKSNKKENKYKK"
     gene            10448..11275
                     /locus_tag="PC700_003840"
     CDS             10448..11275
                     /locus_tag="PC700_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2583045.1"
                     /translation="MKIKDNKAVFHVFIRIFLIAVLILTGTLLEEKINNVPVENISSK
                     GENTLELLSQSNTLGEIIYKASQEETKGPGATSEKVAYITIDDGPSKYTNALLDILKE
                     NDVKATFFMLNQNMKNHKEEVRRVLEEENSIGFHSVSHDIHKLYKSPEVTVGEFNTCN
                     NTLQEITGQSSKLIRLPYGSKPYMPEGSYNKLMANDYLIWDWNLDTQDWKSSTSQIVS
                     NILYYGRKRDELVVLIHEKEQSLNALNNIIRILKERGYTILPITENITPKNFWQENL"
BASE COUNT         4418 a         1266 c         2044 g         3643 t
ORIGIN      
        1 tatctttaat atatgttaaa taataaaata gttacataat aagattaaga atttcttaat
       61 aaattctaat gtaaaaaatc atataaaaaa attaaattta gtataatata tataaatata
      121 tatagaaagg atgtttaaag tctatgagtg gaaaaatttt agttgtagat gacgaacgtg
      181 atatagctga tttaatagaa gtctacctta caaatgaaaa ctatattgtt tataaatttt
      241 attctggtct tgatgcacta aactgcatta atttaaatga atttgacctt gcaattttag
      301 atgttatgtt gccaggagta aatggatttt ctttatgcca aaaaatccga gaaaaatata
      361 cttatccaat cattatgtta acagctaagg atgaagaaac agacaagatt acaggcttaa
      421 ctttaggtgc agatgattat gttactaaac ctttccgacc tttggaactt gttgctagag
      481 tgaaggcaca gattagaaga tataagaaat acagtggaag tttacaatct ggtgaagaac
      541 tttcacatat tttttctttt ttaggtctgg aattgaatac ccaaacatat gaatgccatt
      601 taaatggtaa agctctttca cttactccaa cagagttttc tattctacat gtattattga
      661 aaaataaagg ctctgtggta aattcagaaa atctttttca tgctatctgg aaagatgaat
      721 attacaataa aagtaacaat actataacag ttcatattcg ccatcttcgt gaaaaattag
      781 gtgatactgt agatgaagca aaatatataa aaacaatttg gggtgttggt tacaagattt
      841 gacacctatt tttcttgtca agaagggatg tagtattatg aaaaagatta ataattttac
      901 ttataggaaa ctaagagata aatataaatt tataaaaaga gaaaaaaata cagaggcgta
      961 tttaaagtat aaattatata taatgttagc tatctataca ggtatagtat tggttttaat
     1021 gtttatggct gattatacgt tttcaaattt ttcaaatcct cttgttaaat ggcttaagtt
     1081 gagaagagat gtattatttt ttttggtttt tataattgga tatatcacta ttttcaattt
     1141 ttattggaaa aaaccatgga attatttaag agaaatcatc aatgcaacac atactattta
     1201 tcaacaaaat gatgaattgg ttgaactatc taagccatta aaggaaatag agcaacaaat
     1261 gaaccaaatt aagatgtctg ttttgttaag tcaacaagca gtgaaagagg ctgaaaataa
     1321 aaagaataac cttgtgatgt atctagcaca tgatataagg acaccactta cttcagttat
     1381 tgggtatttg agcttattaa aagaaatacc taatatggat aattctcaaa aatctaagta
     1441 tactgaagtt gcacttgaca aagcacaata tttagaaaaa cttatcaatg aactttttga
     1501 aataacacga tataattcta atcaaatcaa aattaatttc gaaaaagtag acctatatta
     1561 tatgttactt caattaaagg atgaatttta cccattgctg tctgaaaaag gaaatagagc
     1621 cattctaaaa gcaaatgaaa gtttaacaat aaaagcagat tctgaaaaac ttgcaagagt
     1681 gtttaataat atcttgaaaa atgctattgc ttacagctat cctaatacag aaattattat
     1741 ctcagcggtt cataaagaca gcaaggtaga tattgctttt agaaattatg gacagacaat
     1801 tccagaagaa caactttctg ctatatttga aaagttcaat cgtttagatg aagctagaaa
     1861 atctgatatg ggtggtgcag gtttagggct ttctatagct aaggaaatta taaatttgca
     1921 tggtggtgat attactgttc aaagtaaaga tgaagtcaca acatttacta tatcgcttcc
     1981 tacatctatt tagtaaaata taaatcaaat attaggattt tcttaagaaa ttatctattt
     2041 aatattaaaa ttatgagtgt ctttgtttag taaaataaat actaagcaag acacttttgt
     2101 ttttgcaggt aagtagataa agagaggagt ttcaaatatg gaattaaaaa ttgaacatgt
     2161 gacaaaaaaa tttaaggata aaattgctgt agaagatgtt tgccttacct taacaccagg
     2221 tgtgtgggga tttctaggag caaacggagc aggtaaaact accttgatga gaattattac
     2281 agacattatg aaacctacca atggcaaaat tctgtataat ggaaaaaata tttatataat
     2341 gggtgctgag tacagaaatt tactgggatt tttaccacaa gaatttggtt tcccacagga
     2401 tttttctgta aaagattatt tagaatatat agcagcattg aaagatgtac caccaagaga
     2461 agtaaaagga aaaattaata atctattgga aattcttaca ttatctgatg taaaaaataa
     2521 aaagattatt aaattatctg gtggaatgaa acgacgagtg ggaattgcac aagccatgtt
     2581 aaatgaccct aaaatcctta taatggatga accaacagca gggcttgacc ctggtgagcg
     2641 agtgagattt cgtaatttta tttcagaatt ttctcataat cgtgttgtcc taatatctac
     2701 tcacattgta tctgatattg agtacatatc aactcaaaat gctattatga aaagtggaaa
     2761 aattcttgat gtgggaacaa ccgataaact ggttaagaaa gtagagggca aagtatggga
     2821 atgtcttata tcaaatagag atatttcaaa atatgaaatg aatttacgta taatcaatca
     2881 gcgtagtgaa aaaaacaatc aagtatctat tcgttacttg gcagaaaagt cacaaattga
     2941 taactctatt gctgttgaac cacgactaga agatttatat ttatggttat ttccagaagc
     3001 agtttatagt aaggagggga aataaatgcg tattttttat ttggaaatta aacgagtgtt
     3061 gcactcacgt cgtacattaa ttttattatt gttggcattg gttttatctg cattgatggc
     3121 ttttcttcca attacttacg agggtatcaa ttatttagat aataatggca aggtagttga
     3181 attgagtggt ttagatgcta ttaaatataa aaaaacaatt tatgctaata atagtgaaat
     3241 tagtagtgga gttgtaacac caaaaaaatt aaagcatgct ttaaatatat ggaaaaaaac
     3301 agaagaaaag tatggtaaag taggtagtga agatttccca ctagatattt attctaaaag
     3361 tattttacca atacgcccat tgttgaatag acttcctgaa gcatttgcta attcagaaac
     3421 aggatttgca acagaattat cagaaataaa tacaaatgat ttagattctt tttatgaaca
     3481 atgcaaaact catttaaatg atattatgaa gattgaacga caaacttcca gtgcacaaat
     3541 gaaagctcaa gaactttata gaaaagtaga tactccattt gagatatacc caggttattc
     3601 taaagatgct ttcgattata taatatttta catttttata ttattaatta tatgtgttgc
     3661 aattactgca cctacttttt ctggtgagta tcagacacaa tcagatagca tccttcgttg
     3721 tacgaaacat ggacatactc gtctagcgtt tactaaaata ttagctctat tttctatatt
     3781 tatagtaatg tttgttgtat gtatttctat ccatttggca atttctgatt tagcttttgg
     3841 tactgaatgt ttgaaaacct catttcaaat gttgttttct gtcattagtc tagtaaatat
     3901 aaatttacta caagcacaga ttcttatagc tattggtggt ctaatttcaa tacttgcaat
     3961 ggttagttta acattatttt tatctgcaaa atgtaaggat tctcttactt ctatgctgat
     4021 tgctttttct atttgcttaa ttcctatggg aataggtgtt agttggattg gatacctatt
     4081 accaccaggg ggaattggtc ttaagattag tttattatat caaatgacat catttaattt
     4141 cttacactta ggaggtatga gtttttggac accacacatt atactgtttt tctcagtgat
     4201 agaaatacct atttttatat ttttggctat tcgtacctat tgcaaacatc aagtatcata
     4261 acaatttgat agaaataata gtgatataaa ctagaggata cgataattat atatgtagta
     4321 taaaagttaa atttagttaa caaatcttaa tagcagatat aggtaataaa ttatacataa
     4381 actgtattat tatctatatc tgctatttta atatagagaa acaacagtat attgtaaagt
     4441 aaatgtcaaa tatagtttta aaagtaaaat taatgttaaa aaagtaaata taattttaca
     4501 cttgcttaac ttaatcctaa tatagattta acttaaaaaa agtagaataa aagtacagga
     4561 aatttgatga agacgaatta taaaaaatta aattttgaat acagaacgaa aatcgataag
     4621 tatattttaa tgggagggta ttatgatagt aaaatctaga ttagtaggtt tagaaaaaaa
     4681 tgacaataat ggcaataaga caaaatacgt aattgaaaaa gtagcaaaaa atatttttct
     4741 acttagtgct ttagtagcag ttgcaagttt acttttgata ataggattcg tattttataa
     4801 aggcttaaga ccatttatat ttgaaggata ttcatttata gactttttaa ctggtgcaga
     4861 ttgggttcct tctgccaata aatttggaat atcatctatg atagtagcat caatatttgc
     4921 aacacttgga gcacttgtga taggagtacc tgttggtata ttaacctctg tatttatagc
     4981 agaagtagca cctaaaagat tagcaaaaat aatgtcacca gcagttgaac ttttagcagg
     5041 tataccttca gtattatatg gtgtatttgg tcttgcagtg atagttccaa ctatacaaag
     5101 tatatttaat cttccaaaag gtcaaagttt gttagcaata gtaatagtat tagcagtaat
     5161 gatgttacca acaataatat ctgtatcaga aacagcaata agagcagtac caaaagcata
     5221 caaagaaggt tcattagcac ttggtgcatc aaaaatagaa acaatattca aagttgtact
     5281 acctgctgcg aaatctggag tattagctgc agtagtatta ggagtaggaa gagctttagg
     5341 tgaaacaatg gcagtaatac ttgttgcagg aaactcacca gttatgccaa ctgcaataac
     5401 agatagtgta agaccactta caactaatat agcacttgaa atgggatacg catttggtac
     5461 acatcaagaa atgttatttg caactggagt agtattgttt acatttatat taattttaaa
     5521 cttagtttta aataaacttt caaataaggc ggtaaattaa gatgagaaag ttcaaagaga
     5581 atctattgaa gtcattagta tatctatcag cactatttac aatagtatca ttagtcatta
     5641 tagtgggatt tatatttata aagggaattg gcaacatgaa ttttaatttc ttatttagca
     5701 attattcagc atcaggtgat ggtggtatac ttccaatgat aattacaaca ttatatacag
     5761 tagtagtttc tatagttgta gcaactccta taggaatatt ggctgctata tatttacaag
     5821 aatatgctaa aaaaggaaaa gcagttacag ctataagatt tgcaactgaa agtttatcag
     5881 ggataccatc tatagtatat ggattatttg gaggaatttt ctttgtagtt acattaaaaa
     5941 tgggatattc catagttgca ggttcactta cagttgcaat aataatatta ccagtaataa
     6001 ttcgtacaac agaagaagct ttaaaaacag ttcctcaagc ttatagagaa tcatcattgg
     6061 cattaggagc tacaaaattt caaactttat ataaggtagt acttccaagt gcaataccag
     6121 gaatcttatc tggagtaatc ttatcagttg gtcgtataat tggagaatca gctgcaatat
     6181 tacttacagc aggtactgta gccaagatgc caggtggaat atttgatagt gcaagaacat
     6241 taactgtaca ctcatactta ctgactaaag agagtggaga tatagcaaca gcagccagta
     6301 taggaattgt tttaatagta attgtattag cactaaacat gctagcaaga tttgttgcta
     6361 agaaattaaa taaagctaat tattaaattt aagaggtgtg aataatggaa ttaatcgata
     6421 agataaaaat gagtgtaaaa gatttggatt tattttatgg tgataagcaa gcattaaaga
     6481 aaataaatat ggatataaaa gagaataagg ttactgcttt aataggtcca tctggatgcg
     6541 gaaaatcaac ttttataaga acgttaaata gaatgaatga cttaatcgaa gatgtaacaa
     6601 ttaaaggaaa catttctgtt gatggagagg acatatatac ctcagatgat gttataaatc
     6661 ttcgtactaa agtaggaatg gtatttcaaa aaccaaatcc atttcctatg agtatatatg
     6721 ataatgtggc atatggtcct cgtacacatg gactaagaga taagaagcaa ttagataaaa
     6781 ttgtagaaga aagcttaaaa ggtgcagcta tttgggatga ggttaaagac aggcttaaat
     6841 catctgcact aggactttct ggtggtcaac aacagcgtat atgtatagca agagcaattg
     6901 ctatgagacc agaagttatt cttatggatg aacctacatc tgctttagac ccaatttcaa
     6961 cattaaaagt agaggaactt atagaggatt taaaaaaaga ctacacaata gtcatagtta
     7021 cacataatat gcaacaagct gctcgtatat cagatgaaac agcttttttc ttaaatggag
     7081 aggttataga atttagcgat acaaaaacta tgtttaccac tcctgtagat aaaagaacag
     7141 aagactatat aacaggtaga tttggttaaa atatataact ataaaggagt tagacaatgg
     7201 tcaataatag tttagatata agtattgata ctttaaaaga acaaacagta cttatgatgg
     7261 aaaaatgtga agaagcaata gaaaagtctg tgacttgtat gatagagaag gatttagagg
     7321 gtgcaagaaa agtagtagaa gatgatgaag aaataaataa attaagagaa gaaataagag
     7381 ataagagtat agagctaata gtattaaagc aaccaatggc aagggactta agatttattt
     7441 attcattagg taatatatcg atagagttgg aaagaatagg agattatgca gcaaatatag
     7501 ctagagaaat gttgaagata ggtggagagg cttatataaa agagctgata gatattccta
     7561 agatggcaaa agaatgtaag tctatgatgt ctcagactag agaagcttta atcttatttg
     7621 atgcaagtat agcttatgat gttggaacta aagatgatat tgtagatgga ttgtatgaac
     7681 aagtacaaat tgattgttta aaaataatgc atgaaaattc aaatactata gaccaaggag
     7741 taaaacttct acttatagga agataccttg aaagaattgg agaccatata acaaatgtat
     7801 gtgagaagat aatttttgca attgagggta aaatggttga gataggataa aaaactgctt
     7861 taaagcagtt tttttattgt ctaaattaag aaagaattga taaaaaaata tgattaatgg
     7921 gtaaatttaa aaaattgtga tatactagga acatattaaa tatgaggatg cgtattttat
     7981 ataaggtgct ttttaggagg tggaactttg aaacttcaaa agatgggtat gaggactata
     8041 aaaacaggaa ttagtgtcac tttatgtatg attcttggta actatctagt agagaatatg
     8101 ttttattctc tagtagcatg tataatttct gttcaagata cggtaaaagg ttctttaaag
     8161 acaggaatca atagagtgga aggtactatt ttaggtggta taataggatt catatttgcc
     8221 atcattaaag ctggagaccc ttttttatgt ggtctaggag taatgtgctc aatatattta
     8281 tgcaatctgt ttaaaataaa gtcagtagta gttgcctgtg taacctttct ggctatacat
     8341 ctaggtgtag gaaatagtga ccctgtgtat tattctttac atagagtgtt agatacatct
     8401 gtaggtgttt tgtttggagt acttgttaat tattatgtgg ctagaccaaa ttatctaaat
     8461 agtgttatag atgaatttaa aaagatagaa gaactttcaa tgttacttat tgaagataaa
     8521 attcttagaa aagaaaattt aaatgtagag aaatttgaag aagaagtaga aaaattagaa
     8581 gaaatatatt caaaacttat tgatgagttg gattatagtt tgagtgaaac taatatagaa
     8641 aaaattgaga atgccttaag gatatgtaga gaaatacatt ttcatatgca gtctatagag
     8701 ttacttgaaa ataaacttta tttgaaagag catacatata aataccttaa gaaaacatat
     8761 aatgtggata agctagactg gaatatagat gaagaaaaaa gccccgtatt caactatcat
     8821 ctaatgaaaa taatgattga aatagagttg ttgagaaagc taaataaatc atattaataa
     8881 tagtagttaa ataataaata aaaaatagta tctttgtaaa tattaaacat atataagttt
     8941 aatttatgga ggtactattt tttatgaata ctagaaatga caattctaaa atactagaaa
     9001 tatataaaat gaagcttttt ggcgaacttg taaaagcagt gtggaatgat gatatagagg
     9061 atattgaaaa cttacctaat aaaattttaa atgaagattt aaaggataat ttaaataatt
     9121 attttagtgg aaaatttgat aaagatacta tggtaaattt aattagggta actatgggat
     9181 tggatgtgac agaagataca gataaaaatt taaaagagtc actacatgaa gctgtaaatt
     9241 tagatgaagt caaaagacca ataatatctg taatatcaga tgcttgcaaa tattgtgatg
     9301 aaaataataa ggaagatgag tcatgtaaga taagaagtaa gcatattaat tgtaatgagg
     9361 aagatgtatg tagtgcttgt ggtgattgtg tatcaagatg taagttgggt gcaatttcag
     9421 ataaaataga atttatacca atgataaaat tgttaaaaga tataaagagc cctgtatatg
     9481 caatagttgc accagctttt gtaggacagt ttgggaaaga tgtaagccct ggtaaattaa
     9541 gaactgcatt aaaaaagata ggatttgaag atatgataga agttgctttg gcagcagata
     9601 tgcttactgc aaaagaagca tatgattatt atgagcatat gaaaaaggac gaagaaggat
     9661 gctttataac tagttgttgc tgtcctattt gggttagttt gatacaaaat agttttcctc
     9721 agatattgga gaatgtatca ccatccgttt ctcctatgat tgcttgtggg agagctgtaa
     9781 aaattttaaa tccagatgct aaagttgtat tcataggtcc ttgtatggca aaaaagaaag
     9841 aagcaatgtt agaagatata aaagatgcag ttgattttgt gcttacattt aaagagttgc
     9901 aagaaatatt tgatgcatta gagttaaatc cagcaataat ggatgagagt aatagagtag
     9961 aggcgtccct ttcaggtaga gtttatgcta gaacaggagg tgtaagtaaa gcagttaagt
    10021 tatcagtcag aagaattgac aaaaatataa aatttgaaga aaaggcattt gaaggaacaa
    10081 aagagtgcat ggacggtcta aagaaagttc taaataaaga aatagaagct acatttatag
    10141 aaggaatggg gtgtgtagga ggctgtgtag gaggtccgaa gcgtatttta agtgtggaag
    10201 aaggtactaa gaatgttgat gaatatagtg aaagtacgaa tatggaaact ccatttgata
    10261 atttaaatgt attacaattt ttaacttctt tgggaatcaa aagagtagaa tctttaggag
    10321 aaaaagaaga agaaaaggtt aaaaaaatat ttagtagaga tataaaaagt aataagaaag
    10381 agaataaata taaaaaataa ctttttaagt cttaaactta tgcttttata aaagggagat
    10441 atgggggatg aaaattaaag ataataaggc tgtttttcat gtatttataa ggatatttct
    10501 tatagctgtt ttaattttaa ctggaacatt attggaggaa aaaattaata atgttccagt
    10561 tgaaaatata agttcaaaag gtgaaaatac actggaactg ctttctcaaa gtaatacttt
    10621 aggagaaatc atatacaaag catctcaaga agaaacaaaa ggtccagggg ctacttctga
    10681 aaaagtagca tacataacaa tagatgatgg tccatcaaaa tatacaaatg cactacttga
    10741 tattttaaaa gaaaatgatg tcaaagcgac attttttatg cttaatcaaa atatgaaaaa
    10801 tcacaaggaa gaagtaagga gagtcttgga agaggaaaac tcaataggtt ttcatagtgt
    10861 aagtcatgat atacataagt tatataaaag cccagaagta acagttgggg aatttaatac
    10921 atgcaataat actctacaag aaataactgg tcagagttca aaattaataa gattaccata
    10981 cggaagtaaa ccatatatgc cagaaggttc atataataaa cttatggcaa atgattattt
    11041 aatttgggac tggaatttgg atacacagga ttggaagtct tctacaagtc agatagtaag
    11101 taatatatta tattatggaa gaaaaagaga tgaattagta gtattaattc atgagaaaga
    11161 gcaaagttta aatgctttaa ataatattat aagaatctta aaagagagag gatatacaat
    11221 attgccaatt acagaaaata taacacctaa aaatttctgg caagaaaact tataataaat
    11281 ttaaatatag taaaagataa agtaaattat ttataaaata aaacatataa tataaaaaat
    11341 acgaataaga ggtatactgt aaggataata t
//
LOCUS       DAMJWP010000473         8195 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.guided.165, whole genome shotgun sequence.
ACCESSION   DAMJWP010000473 DAMJWP010000000
VERSION     DAMJWP010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8195
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.guided.165"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            82..798
                     /locus_tag="PC700_003841"
     CDS             82..798
                     /locus_tag="PC700_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2583046.1"
                     /translation="MILIVILSIFIIASINIIILGKENIRDIVIPLTLFIVSDFLFLC
                     GNIICRGGVMLLFIGLFAIPLMLIAFIWICVHMIKLYYKKDLNKRKMIGCIFSIFISI
                     GTIFIPSPSESFRFKLYEKDYSVVAKAILKVYDEKKLSSESVFYIQSDKNELEKIFSK
                     EVIKKMKKLNRNLDVVKISGNRNLVYFFFGAELQSIDGIVIFKDLKHVKPKLDFINRS
                     IQAPRLEYITNNVYYFKGEL"
     gene            826..1764
                     /gene="blaCDD"
                     /locus_tag="PC700_003842"
     CDS             826..1764
                     /gene="blaCDD"
                     /locus_tag="PC700_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HDF2583047.1"
                     /translation="MKRKNNFIWIVILLVVVAMGVYYVGRKHNNTKQKHDKNHINSEL
                     NKSKVENNDKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETRRSPCS
                     TFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYYKKLID
                     KVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQTMAKI
                     FEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGMLEREGD
                     TYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            2108..2791
                     /locus_tag="PC700_003843"
     CDS             2108..2791
                     /locus_tag="PC700_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2583048.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            2788..5448
                     /locus_tag="PC700_003844"
     CDS             2788..5448
                     /locus_tag="PC700_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2583049.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIRLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPIAQKSKGESFKKISETTLTIVGKVDVSTSSVIPAYTALGYLDRTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKMS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYINSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKSIVSSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYDTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKTVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNTRSRRQEFAMLRSVGLSPE
                     GIKKMLVLEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(5629..6810)
                     /locus_tag="PC700_003845"
     CDS             complement(5629..6810)
                     /locus_tag="PC700_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2583050.1"
                     /translation="MKTPRNINSTVVFIIFCLIITFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVVYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(6810..7472)
                     /locus_tag="PC700_003846"
     CDS             complement(6810..7472)
                     /locus_tag="PC700_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2583051.1"
                     /translation="MAKILLLEDDDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCGNISLNKVEVKAYLDNVEVLLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DS"
     gene            complement(7617..8159)
                     /locus_tag="PC700_003847"
                     /pseudo
     CDS             complement(7617..8159)
                     /locus_tag="PC700_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
BASE COUNT         3044 a         1027 c         1298 g         2826 t
ORIGIN      
        1 ctattgtaat atttaaatat tacaatagta agttttaaat agagaataaa taattacctc
       61 acaaactgaa aggaggacat aatgatacta atagttatat tgagtatatt catcatagcc
      121 tcaatcaaca taattatatt aggtaaagaa aatataagag atatagttat ccctttaact
      181 ctatttattg taagtgattt tttatttttg tgtggaaata taatatgtag gggaggagta
      241 atgctactat ttataggttt atttgcaata ccattgatgc taattgcatt tatatggatt
      301 tgtgtacata tgattaagct ttactataaa aaagatttaa ataaaagaaa aatgattgga
      361 tgtatattta gtatctttat aagtataggt actatattta ttcctagtcc aagtgaaagt
      421 tttagattta aactttatga aaaagattat agtgtagtag ctaaagctat tttgaaagtt
      481 tatgatgaaa aaaagttaag ctctgaatct gtcttttata ttcaatctga taaaaatgaa
      541 ttggagaaga tattttctaa agaagtaata aaaaaaatga aaaaactaaa cagaaattta
      601 gatgtagtta aaatttctgg gaatagaaat ttagtctatt ttttctttgg agctgaatta
      661 caatctattg atggtattgt gatttttaaa gatttaaaac atgttaaacc aaagttagat
      721 tttataaata gaagtattca agctcctaga ttggagtata ttacaaataa tgtatattat
      781 tttaaaggtg aactataaac ttatgtagag gagatggtat aaagtatgaa aagaaaaaat
      841 aattttattt ggatagttat tcttctagtt gttgtagcta tgggagtata ttatgtagga
      901 agaaagcata acaacacaaa acaaaaacat gataagaatc atataaactc tgaattaaat
      961 aaatccaaag ttgaaaacaa tgataaaaac aaaaaagaaa aaattaatat ggttgactat
     1021 agtgactgtt ttgaaggtat tagtggagga gctatttttt ataatactaa aaataaagaa
     1081 tataatatat ataacaaaga attaattgaa acaagaaggt caccatgttc tacttttaaa
     1141 atagtttcaa ctttaattgg tttagaaaaa ggggtaataa actcaaaaga atcagttatg
     1201 ggatatgatg gtacagagta tccaaataaa aattggaata aaaatcttag tttagaagag
     1261 gcatttaagg agtcttgtgt ttggtactac aaaaaattga tagataaagt tgatgctaaa
     1321 tctgtccaaa acattttaga tgatttaaag tatgggaatt gcgatattag cgaatgggaa
     1381 ggagatttaa aaaatggaaa gggacattta aatggatttt ggttagaatc ttcattacaa
     1441 atatctccaa aagaacaagt gcaaacaatg gcaaaaatat ttgagggaga tacaaacttt
     1501 aaaaaagagc atattaatat tttaagagat attatgaaaa tagatgtaaa tgataaaaac
     1561 ataaatgtat atggaaaaac tggaacagga tttgatgaaa aaaataaacg tgtggatgca
     1621 tggtttgttg gtatgctaga gcgtgaaggt gacacctatt attttgctat taaatcagat
     1681 gattccaata aagaaattac agggccaaag gtaaaggaaa ttgcaattaa tataattaag
     1741 aaatattata gtgtaaggga ataaacttaa ttttatttgt aaatattatt tacctatagt
     1801 ctgtaatatt tattaaataa ttttactaaa ttgagtctat atagagcaaa actcctttag
     1861 tacataaaat acttgctatt gattattaaa ttgaatacct atagatttaa agttaatacc
     1921 ttttaaagac tatgtaatat attaaaagta taaataaaat atattgcata gtttttgttt
     1981 tatattataa ttttaaataa tgtgcaaaaa ctaaacaata tttttaatag taatttatat
     2041 ttgtattcat gtcactataa tgtcatttta ggttgctaaa atttaaccat aaaggagatg
     2101 attgtatatg gaaatattaa aatgtgaaaa tctcacaaaa atatatggtt caaatcaaac
     2161 cagagtgaca gcccttaata atgtaaattt atcagttcaa aaaggtgatt ttgtatccat
     2221 tgtaggagca tctggttctg gcaaatctac attgttacat ctgcttgggg gagtagatag
     2281 acctacatct ggaaagattt atgttgaaga tacagaaata tcaagtttaa aggaagaagc
     2341 tcttgcagtt tttagacgta gaaaagtagg tcttatttat caattttata atcttatacc
     2401 tacccttgat gttagaaaaa atatattatt acccatgtta cttgataaaa gaaaggtgga
     2461 tgaggataga ttctctgaga ttgtatctat attgggatta tctgatagat taaaccatct
     2521 tccaagtcaa ctttcaggtg gacaacaaca aagggtatct atagctagaa gtttaatcta
     2581 tagaccagct attttattag cagatgagcc aactggtaat cttgatagaa aaaattcaga
     2641 agaaattgta gacttgttaa acttatcaaa taaacgattt aatcagacaa tacttcttat
     2701 tactcatgat gagaaaatag cactagaggc aaatcgtatt gtaactatgg aagatggagt
     2761 gattgtctct gaaaaggtgg tgaagaaatg aatattcttc aagaatacac cttagacttt
     2821 gtacgacgca ataaacgtag tagtatagct ataatgttag ctatattact tactacaacc
     2881 atgatgtctt ctttgtgtgg actgttgtat accatgtgga cagattttat aagactttca
     2941 attgagaaag aaggtaactg gcatggtgaa ttgtttgact atacttatgg aagtgactta
     3001 ccattaattg aaaatttttc ttcagttgat gatgtaatgg taaaaggaca atggtatgta
     3061 ggcaaaatta atgatgagaa aagagattat ataatttata gaaatgctac atcagagtat
     3121 tggaattcta tgccagaaaa ggatactata atagagggtc gtcgaccaaa taatgctaat
     3181 gaaattgcat tatcaaaaca atattttgag aataatccaa gtgtaaaaat tggggataaa
     3241 attactcttc caataggaga tagaatccta aatggaaagc ctcttgaacc catagctcaa
     3301 aaatcgaaag gggaaagttt taaaaaaatt tcagagacta cattgacaat agtaggaaag
     3361 gtagatgttt ccacttcatc agtaatacct gcatatacag ctcttggata tcttgataga
     3421 acaagtataa aacctaatga tagtataact gtgtacttgc gatttaaaaa tataagagac
     3481 acatacaagg aacttccaaa acttgctaaa tcattggggt ggaagactaa tgaatatgga
     3541 aaatacaatc ttaaatataa tagtaattat cttttaaaaa tgttagtctt ttccccagaa
     3601 cagaaagcat ctatgagcag tatatcaaaa ttttcaactc ctattatgta tcttacaata
     3661 gcaattttta cagtagctgt gtttgtaatg gtgatttaca acgcgttttc actatctgca
     3721 aatgctagac ttactcaatt agggatatta tctagtgttg gtgcatcacc aaaacagatt
     3781 aaaatgtctg tagtgtttga aggttttttg cttacaatta taccattgcc aataggttta
     3841 tttttaggat ggttattgtg taataggctt atagtttata ttaattcagt taattatcat
     3901 actgatgttc cagaagtggt atttacttat ggtattcctg cttttctgcc agcagtttta
     3961 cttacaatag taacagtctg gatttctgca cttataccag cacgaaaagt atcaaagata
     4021 tctcctatag aagcaatacg acaaggagat agtgttaaaa tcaaaaagtc gcataaatat
     4081 tcattaggaa agatatttgg aatagaagga gaacttgcat caaacgcact acgtgctcgt
     4141 aaaaaatctt atcgtactgc aacaatatct ttaacattat cttttttatt acttacatgt
     4201 tttttgcata taattgcaaa tcaagaagga gcaaaagctg tttatggaag tgataattat
     4261 aaggaccaaa gagatatagc agtttatttg gaaaatagtg aacctgtaga gtctgctttc
     4321 attgaaaaat tagataatac agaaggtgta aaaagttcac tatattatca aaaaatgtct
     4381 gcttccactt ggctgactga aaaagatgca tcaaaagaac ttgcgaaaaa tggaggattt
     4441 aaaagtatag tttcatctaa aaaatactct cctatagaac gtgatggcaa gtttcgtata
     4501 atttcaaata taattgggct tgatgataag agttttacag aatattgtag gcaaatttct
     4561 gtagacccaa agttatttta tgatacaaaa aatccaacct caattgtata taataaggag
     4621 gaagatatta atcgtagtac acgtagaaat aaagtatata tagattattt aaatctttca
     4681 ataggagata acattaaatt aaatgaaaaa gtatatgacg aagataaagg ggattatact
     4741 tacaacatga aagtgggagc tattacaagc acacttccta aaacagtaga tactagttca
     4801 aaatatacat taatgcaaat agtgccaatg tctatggtgc aggaaatatc taaaaattat
     4861 gatgaagtaa gtaggttacg ttcaaatagg ctgataggag tatttaaagt aaataatcgt
     4921 gaggacattc ctagagtaag agaaaaatta gagtccattt ataataaaga ctatggttca
     4981 ggagattata gtattgagga tgttttagaa agtgaaaaac aaaaggctag tggaataaag
     5041 accatgaatt taatagtagg ctttattaca ggtttattgg ctatgattgg attatcaaat
     5101 gtacttgcta cagtgtcagg aaatactaga agtagacgac aagagtttgc tatgttgcgt
     5161 tctgttggat tatctccaga aggcataaaa aagatgcttg ttttggaagg tttgttctta
     5221 ggaattacac cattactact tagtattcct gtgcaaatag gaatagtgta tgcttttcta
     5281 cgtataaatg aaatatattt tatagagtat ttaccatttg cacctatatc tacaataata
     5341 ggttttacta tattgatact ttttattgta atagcatcat atatgacagg atacaagcag
     5401 ttgaagaatg aaaatatagt agaatctata aaaaatgaaa caatataaac aaaagctatt
     5461 tgaacttatt taaaggtttc gatttaagac tacttacaag tatagggata gttaatttgt
     5521 ttgtttaata aaaccttaaa tgtaaataga agtatattac taaaacaata gataacatag
     5581 aatatagata tagctggatt ataggtattt tacctataat ccagcatttt atgtgtaaaa
     5641 tttaattatg aatcttgcac caccattatg gcaattttct gcatgtagag taccattttg
     5701 acgttctata atagactttg atagagcaag ccctatacca atactgtcct tactagcatt
     5761 tttaccacga taaaatctat taaaaatata tggaagttct tcctgagcaa accctttacc
     5821 attatcttct atgataatct caacatatag aggattttga ctataataag caactatata
     5881 tccaccatct tctatatgct cactacaatt tttaagaata tttaaaagag cttctaaagt
     5941 ccaattaata tcgatttcaa attctaccga aggagaagcc tcgatagtaa atgtctgatg
     6001 tttttctctt aaagaacctt ctataggctc aattgcataa gtaagaagag aatgtacatc
     6061 tataagtttg ctttcaaata ttaaagtact tgcatccaat tttgataatg taagtaaaga
     6121 tgaaactagt ctctcaagtc ttgagatttg atttgtaagt ctgttaatat aatctatttc
     6181 ttcatctgga caattttctg aaagtaattg tgccataagt gacatagaag taattggagt
     6241 cttaagttga tgagcaatat ctgcaagatt atctgaaaga gtttgtcttt cttttaaagc
     6301 aaattctttt gaacaacgaa gttccccaac tgttttatat atctcatctt caaggtgaga
     6361 aaattcatct tcacatcgta aaagtacaga ctcttttcct aaattcactt cttctaagta
     6421 ttgagtaaga ttgtttattc tactcagttt ttgcttattt gttctatgtt tgattatata
     6481 tacaactgta gccattgttg aaattatacc aaacgatatc actagaaaat aaaaaaagtt
     6541 tttactccaa aatgtacttt tagaatatcc atgttttgat aaaatttcat ttcctgtttt
     6601 aaaatcctta tcactagaat ttttcagtga ttttagtata ctttctgtag acttaggatt
     6661 gtactcatat gatgcaccaa gagccttaga cagtacattg tattcattaa aagaagttat
     6721 agttgttaga attacactta ataaaaaagt tataattaag cagaatatta taaaaacaac
     6781 tgtactgttg atatttcttg gagttttcac tagctgtcct ccattctata tcctatgcct
     6841 cttaatgttt ttatacaagg tggatttttt aatttttctc taagacgctt tattgtaaca
     6901 gtaagagtgt tatcatttac gaaattacca tctacatccc agagcatttc tagtaacttt
     6961 gtacgggtca tagctctatt tttattttcc ataagactta gaagaagttt atattctcct
     7021 gctgtcaaaa gtacttcaac attatctaaa tatgctttga cttcaacttt attcaagctt
     7081 atatttccac atgtaatagt attgttagta tttgaatgag ttcttctaag aactgcatta
     7141 attctggatt gaagtactga gagcagaaat ggtttaacca catagtcatc tgcacccata
     7201 tctaatccac gtataatgtc tttttcatca tcacggactg tcaaaaatat cactggagta
     7261 tcttctatac ttttaacata ttggcaaaac tcgtatccaa taccatcagg tagatttaaa
     7321 tcaagcagaa ttaaatcaat agttttatca aaattttttt tagcttcagc aatcgtatta
     7381 taacaacata cagtgtgatt tttcttttca agggaagttt ttattccaaa tgtaattgta
     7441 tcatcatctt ctaaaagaag tattttagcc atttactttt agtcctttca gtaatttagt
     7501 tatttcatta actaagttat tagttttatg tttaatattt tttatgttag agatagaagt
     7561 tagagataga aaaaattatc atttaagtat ataaacttta atttataaac ttaggtttat
     7621 gctttaattg aatctattaa aagagcaaga gttgtttcaa taaatttttc ctcatctata
     7681 ttaaagttgt tcaggtatga ctttcctgtt acagatagta aattaaaaaa tcctgtaagt
     7741 gtaaatattg atgaatacat gagagtaggt atttcaatat catgcctaat acttctatca
     7801 ttttttccaa tttcaaacat tccttgtatt tcatcaaaca taaatttatt aaaagaaaaa
     7861 aacttttctt taaacagcat atcagtatcc gatttttcag agtttacaat accaatcatt
     7921 cccataaggc ataatagaga aggttctaca gagtaaaatt tatgaaaagc aaagtatgaa
     7981 agttttattt tttcaaaccc tgttttgcct tttgcacttt ctattttaac attatcccaa
     8041 agtcttttat atcctcttaa gacaacagca aagaataaat cttctttaca tgaaaagtac
     8101 ttgtatatgg tgcgtttagt atattcaact tcttttgcca actcattcat agttgtattc
     8161 tgaacctccc atgactaaag tcacagggtt cctgc
//
LOCUS       DAMJWP010000474         7700 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49288 isolate JH320
            SAMN32366162-rid16591393.guided.178, whole genome shotgun sequence.
ACCESSION   DAMJWP010000474 DAMJWP010000000
VERSION     DAMJWP010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366162
            Sequence Read Archive: SRR22862996
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560852.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560852.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 02:38:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,855
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 27 of 48
            Pseudo Genes (incomplete)         :: 19 of 48
            Pseudo Genes (internal stop)      :: 11 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7700
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366162-rid16591393.guided.178"
                     /strain="NR-49288"
                     /isolate="JH320"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            237..575
                     /locus_tag="PC700_003848"
     CDS             237..575
                     /locus_tag="PC700_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2583052.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
     gene            597..914
                     /locus_tag="PC700_003849"
     CDS             597..914
                     /locus_tag="PC700_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2583053.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            1064..1741
                     /locus_tag="PC700_003850"
     CDS             1064..1741
                     /locus_tag="PC700_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2583054.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     LYYIYNH"
     gene            complement(2069..2836)
                     /locus_tag="PC700_003851"
     CDS             complement(2069..2836)
                     /locus_tag="PC700_003851"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDF2583055.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKVAVMPQNERDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            3642..4274
                     /locus_tag="PC700_003852"
     CDS             3642..4274
                     /locus_tag="PC700_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HDF2583056.1"
                     /translation="MTIPNSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVSPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
     gene            complement(4645..5514)
                     /locus_tag="PC700_003853"
     CDS             complement(4645..5514)
                     /locus_tag="PC700_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2583057.1"
                     /translation="MEYDMPLYRPPSEAYSLIIQVTLGCSHNKCTFCSMYKSKKFKIK
                     PLEVIKNEIDIFRRYYKHVERIFLADGDALIIPMEKLRDIILYIKEVFPECERITMYG
                     SPKSIEKKSDDELKELRKLGVKMIYLGLESGSNEVLEDIKKGFSSEQLIKVGRKVKKS
                     GIKLSATVIAGLGGTKKTHQHAVDTGKMLGAISPDYVGVLSLMVEPNTELYDLLKAGE
                     FTILEDKAVLQEIKEMIKNIDANEKIVFRSNHASNYANLKGVLPDDKQRLIDEIDYYL
                     SNQKLKREEYRRL"
     gene            complement(5611..6468)
                     /locus_tag="PC700_003854"
     CDS             complement(5611..6468)
                     /locus_tag="PC700_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2583058.1"
                     /translation="MSKDVKVKRNKKYDTFSITMELERPLLYDEVENKDDKNNNRKFN
                     IKNKIFKGIGILFVLILLGFFIWINKTYEPQKLAKEALVSNSKVEVTVNENISFTPKG
                     RKVSKGLILYPGAKVEIESYAPLARKIAESGYEVVIVKMPLNLAILGTNKAQKVMDSY
                     NNIEHWVIGGHSLGGVAASNFARDNKLIEGVVFLASYPMGDELKELGKKVISIWGSKD
                     GVVNFKSLVESKQKLPDDTTYVEIEGGNHAQFGDYGKQKGDNDAIISQEKQLNITANS
                     IIKFLKNIS"
     gene            complement(6683..>7700)
                     /locus_tag="PC700_003855"
     CDS             complement(6683..>7700)
                     /locus_tag="PC700_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDF2583059.1"
                     /translation="KSRDEKKAERKSKIPHEFKRLSIYKKNFNLNSIRFSYAVKIALA
                     TAVAGFIMDYFHLRDGRWIMLTVFSLTQPYAENCIQRSRKRIEGTFIGAVIFIVLFSI
                     IKDSTLRSLIVLLAGYINSYVVDYRKLMVCVTVSALGSAVVMGDPNVLTISRISYVAL
                     GAIIALIVNKFILPYDAKTGYQHVIEMYKGIVKNIIDEVNKSIENVADVYYIKNLLLI
                     PSLIEDRLMLINAIYKDKHQEDFLENQKLLISNMYNLYINVNKNKIKDEDVEKILRDT
                     NYISNYNVDKYDEGRSVILESIVNTKSLGDKIICLNLLQTLNGVKEMYRISNITKVSI
                     KEAA"
BASE COUNT         2825 a         1093 c          847 g         2935 t
ORIGIN      
        1 acatattatt aattcgtttt attattgtag tatggctatg aaattatgaa aatatttctc
       61 taattaaaaa tatgatttat ccatatttgt ttattgtaga tacagactat acaaaaataa
      121 atagcactta tttcagtata aaaacatata tagtctatat ttagactaaa aaagtataaa
      181 tagtctatat aaaacaactt aaactatgaa tataatctaa agtggaggga taataattgg
      241 caaatatagg aaaaataata ggagatagaa taaaagtcta tagaacaaaa ttgggatata
      301 gtcaagagtt tctagcagaa aaggcaggtt tacatccaac atatattggt cagattgaaa
      361 gagctgagac aaatacatct atcaatatta taatgaaaat tgctactgct ttagacatac
      421 cacttgaatt gttatttgca aatattatta caacagaagg cgttgataac tctgtacctt
      481 tagaatgtta tgaaatgatt aataaattaa ctgaaaaaga acaaattgca atgctagagt
      541 taataaagtg catcattaaa tacaaagaaa tataagtgag ggaaatagta acaataatga
      601 attatatagg taaaagactt aaagaagaac gaaaaaaagc aaatttaact tcaaaagaat
      661 ttgcaaatat ggttggagta tcgccttggt atataactca aatcgaaagt ggtaaaaaga
      721 atccaagtct taaaacattc ataaaatttg taaatatatt aaatatttct gcagatgttc
      781 ttataaaaga tattacttcc actggaaaaa catatttaga aaatgatata aatgaagagt
      841 taaaagactt aaattcaaga gaactaaatc tgatatcaca gattatagac atcataaaaa
      901 agaataaaat ataattcttt atattttata taaaaaaaaa ggttatttga aataaataac
      961 ctttttttgt ctttttgtgt taacattgtc ttatagtgct aatgagtact attagaccat
     1021 gttctatggc cttattttaa tataaaacga ttggagatag gctatgattg tgattgaagg
     1081 tagtgataaa tttaagattg caaaagaata tattgatgta gaatatactc tttttagcaa
     1141 agtaactttt aggtatgaaa agttgaaatt taaagataat gctgaattgg aaaaaattaa
     1201 gatgtttaaa tataaaaatg gctacatccc taataaatta aacctttctt ttggatatgg
     1261 attctcttct tataaaaagc aaataattag agaaactgta gatactttaa gattgacaga
     1321 aattttttca agcgagaaca tagaagatat taaatttata aaagatggta caaaaaaatt
     1381 agaaattagc atagagaaag ttgtgaaatt taaacgtcga aaaaaaagaa attatgtttg
     1441 ttgctattgc cctgatatgt atagagatat aaaactcgac aaagaatcta tcaataagat
     1501 atacaacaga aaaataaaaa tagaaagaga agttaatatt tttgaagatg aggatgttat
     1561 aataaacaag aaagtattga agtttccaaa gtcttggaca aagaatatgc aaaaatattg
     1621 gttaagtgaa aataagtatc ccatacattc tactgtaatt gatgatgata gatataaatg
     1681 ttgtaatgta caatatacaa aaaatagagt gataatatta tattacatat ataaccatta
     1741 atatacataa ttacaaaaaa ataatgacaa atatacataa tacaaatata tcttctactt
     1801 tagataaaac agttattatg aaaacttact atctataaat aatagaaatg ctagtatacc
     1861 atctataatc taatgtctag taggttttaa tggcatattt taaatatgcc atagttgatt
     1921 tataagattt tctatatcac tttcaaaata cacactaaat agttgctttt cagatataat
     1981 tgcccaattt aaattttact atctgaaaag ttttttatat ttaatttata tcttcaaaaa
     2041 taccgtctaa tatttctata taattaattt aattaatact cttatgtaat agattaagta
     2101 ctgaatttaa ctctttaaat gatatttgcc ctggtgctga aacacttttt gcagctccaa
     2161 aagtcaatgc agaaccaaat atttcaccac acaatctact tataacaccc attcctgaca
     2221 ttgacattgt tattattggt ctatctgcat aaattttaaa catttcattt gtagcttcaa
     2281 gtagcactaa cacatctctt tcattttgcg gcataacagc tactttgggt aaatctgcgc
     2341 ccaattcctg cattctacat aaacgagata caatctcttc tttttttgga gttttattaa
     2401 aatcatgatt agatataatt actttcactt cttttttatg agcaaaatta actacttcat
     2461 ctatgacctc atctcccata aataactcta catctattaa atctactaaa cctgtattag
     2521 aaatttcttt attcaaagta gtataataat ctcttgatat taatttttct cccccttcta
     2581 ctacacttct aaatgtgaat aataaaggga tatcatgtat ataacttctt aattcataga
     2641 gaacttcctt aacttcttta atattttcta cattctcaaa aaaatcaact cgccactcta
     2701 taatatccaa acatgcatct tttaactctt tagcctcttt tataatatct tttttatttt
     2761 tcccaataat tggtacacaa attttaggtc taccttctcc aatagtaata ttttttactt
     2821 gaacttttct tttcatattc tcctccataa tcagaaattc tataggactt taaattctat
     2881 tttctgcata atttaattat acattaaaaa tactatacat tataaaaata ctaatttaaa
     2941 aataattttt attctaattc ttaaaacaat cccacatatt ttagaaaagg tactctataa
     3001 tatacaaaca cttctttttt acctaaattt attttaacat tctataattt tcacaatatt
     3061 ctttaaattt tactcatata aacaatatta ttctggatgc taatagaatt aatctataaa
     3121 tcatagtata attatgcaga aataactcga atttcactac ttataaagag attgaatatt
     3181 aatgaatcta aatcatttgc attacttcag agttttgact aaagttgaac accatacaaa
     3241 agtagctact caattgttta taccccaacc aagcttaagt aatgttatat agattcttga
     3301 aaaagaattt ggtatctttt tatttgaaaa acaaggtaga aatgtttccc tcactaaata
     3361 cggaagtttt tttctaacct atgttgataa agcattaggt taattagaaa aagtttacat
     3421 ataaaatata ttaaattgtt gcaagtttta ttgttttgat atacaatcaa ctaaacatat
     3481 aatctttata aaatactata cagttaaaag tctgttttaa aaaaaaatgt ataaattata
     3541 aatcaaaata tagatactat caaggagtga ttctgtgaag agatgtattg tagtagtgga
     3601 atagtcaaaa acataatata tattaaagga gagatttaaa tatgactatt ccaaattcaa
     3661 ataaggtata tccaagaagt aatgattatc aaactgtata tcttaaaaat gtaataacaa
     3721 gggaaaatat agaagttgga gattacacaa tatataatga cttttataat gacccaagag
     3781 attttgagaa aaacaatgta ttatatcatt accctataaa caatgataaa ttaataattg
     3841 gtaagttttg ctcaatagca tgtaatgcaa aatttctaat gactagtgga aatcactcaa
     3901 tgaaatcaaa atccacatat acttttccca tcttttatga agaatggaaa ctcgatatca
     3961 accatataac ggacgcttgg gacaataagg gtaacattat aattggaaat gatgtgtgga
     4021 taggttatga ttctataatt atgtcaggtg taaatattgg tgatggtgca attattggaa
     4081 caagagcagt agttactaat gacgtctcac cgtattctat tgttggagga gtacctgcaa
     4141 aaattataaa aaagagattt aataatgata caatttcaaa attattagaa attaaatggt
     4201 gggattggcc atataaaaaa attcaatcaa acattaagca tattcaatca ggaaatattg
     4261 ataaattagt ttaatatttt ataatttatt tatattaaac tttactttaa taatcctttt
     4321 ataagagctt tttcaaaatt tgtatattat tttaatatat ataaatttaa tacaacatta
     4381 aataaaataa aaagtaaagt tacttaataa aaagttactt tactttttat tatttaaatt
     4441 cctgttttta ttataatagg taaaattcta ttaaactagt tcacattttc attattattg
     4501 atatattata tatgaatata ccattttgtt ttttaataaa aaaatcatgc tacatcagta
     4561 gtaagcaact attaatgtag catgattttt tgtatctaaa taatcttagt atttacctat
     4621 caatatagat tgatatatca aaaactatag tcttctatat tcttctcttt ttagcttttg
     4681 gttacttaga tagtaatcaa tttcatcaat tagacgttgt ttatcatcag gtaatacacc
     4741 ttttaaatta gcataatttg atgcatgatt acttctaaat acaatttttt cattagcatc
     4801 tatattcttt atcatctctt taatttcttg taacacagcc ttatcctcta gtattgtaaa
     4861 ctctcctgcc ttaagtaaat cgtaaagttc agtattaggt tctaccatta aactaagtac
     4921 acccacataa tctggtgaaa ttgctcctaa catttttcct gtatcaactg catgctgatg
     4981 tgttttttta gttcctccaa gacctgctat tacagtggct gataatttaa tcccactttt
     5041 tttaactttt ctaccaactt ttattaattg ttcactacta aaacctttct taatatcttc
     5101 taatacttca ttgcttccac tttcaagccc tagataaatc attttaacac caagttttct
     5161 taattcttta agttcatcat cagatttttt ctctatagat ttaggagagc cgtacatagt
     5221 aattctttca cactctggaa aaacttcttt tatatataaa ataatatctc ttaatttctc
     5281 cattggtatt atcaatgcat ctccatctgc taagaatatt ctttctacat gcttataata
     5341 tcttctaaat atatcaattt cattttttat tacttctagt ggctttattt taaatttttt
     5401 agatttgtac atactacaaa acgtacattt gttatgagaa catcctagtg tgacttgtat
     5461 tataagacta taagcctcac ttggtggtct gtaaagtggc atatcgtatt ccatattaaa
     5521 atcccccttg taataaaata ttcattctat tatattatta atatttactt taaataataa
     5581 cttataaaat tatatttttt aaattttagt ttaagatata ttctttaaaa atttaatgat
     5641 actattggca gtaatattaa gttgtttttc ttgacttata atagcatcat tatctccctt
     5701 ttgttttcca tagtctccaa attgtgcatg atttcctcct tcaatctcaa cataagttgt
     5761 atcatcagga agtttttgtt ttgattctac aagactttta aaattgacta ctccatcttt
     5821 gctcccccat atagatataa ctttttttcc taattctttc aactcatcac ccattggata
     5881 agaagctaaa aatacaacgc cttcaattaa tttattatca cgtgcaaaat ttgatgctgc
     5941 aactcctcct aatgaatgac ctcctattac ccaatgttct atgttgttgt aagaatccat
     6001 aactttctgt gccttattag ttccaagtat agctagatta agtggcatct ttacgattac
     6061 aacctcatat ccactttctg ctatttttct tgcaagtgga gcataagatt ctatttctac
     6121 tttagctcct ggatacaaaa ttaaaccttt agaaactttt cttccttttg gtgtaaatga
     6181 aatgttttca tttacagtta cttcaacttt actattgctg actagagctt cttttgccag
     6241 cttttgaggt tcatatgttt tatttatcca tatgaaaaag ccaagcaaaa tcaatacaaa
     6301 tagtatacct attcccttaa atatcttatt ctttatgtta aattttctat tattattttt
     6361 atcatctttg ttctcaacct catcataaag taaaggtctc tcaagttcca tagttatact
     6421 aaatgtatca tatttcttat tacgctttac ctttacatct ttactcataa atctaacccc
     6481 cataatattt gaatactatt atagtttatc ctaagtaaat tgttttttca accaatgaaa
     6541 aaaatcaata ttttaactat tttcatataa aattcataaa tatatgtttt ttgtttttta
     6601 tatattattt tcttttagta ttttataaaa caaatagcca tatactttat tttaaatata
     6661 tggctattct ataatttaat atctaagctg cttcttttat tgaaacttta gttatattac
     6721 ttattctata catttctttg acaccattta gagtttgcag taagtttaga catattatct
     6781 tgtcacctag acttttagta tttacaatac tctctaaaat tacacttctt ccttcatcat
     6841 acttatctac attgtaattt gaaatataat tagtatctct caatattttt tcaacatctt
     6901 catcttttat tttattttta ttaacattta tatatagatt atacatatta cttattagta
     6961 atttctgatt ttctaaaaaa tcctcttgat gtttatcttt atatatagca tttatcaaca
     7021 taagtctatc ttcaataagt gatggtatca ataataaatt ttttatatag taaacatcag
     7081 ctacattttc tatagactta ttgacttcat caattatatt tttaactatt cctttataca
     7141 tttctataac atgttgatag cctgttttag catcatatgg taatataaac ttatttacta
     7201 ttaatgcaat aattgctcct aatgctacat atgaaatcct gcttatggtt aaaacatttg
     7261 ggtctcccat aactactgct gaaccaagag ctgaaacagt tacacatacc attaattttc
     7321 tataatcaac tacataagag tttatatacc ctgctaaaag aactattaac gaccttaatg
     7381 tactatcttt tattattgaa aataatacaa taaatattac agctcctata aatgtacctt
     7441 ctattctttt tctagacctt tgtatacaat tttctgcgta aggttgtgtt aatgaaaaga
     7501 cagttaacat tatccatctt ccatctctta aatggaaata atccataata aaacccgcta
     7561 cagctgtagc taaagcaatc tttactgcat aagaaaatct tatagaattt aaattaaagt
     7621 ttttcttata tattgatagt cgtttaaatt catgaggtat ctttgatttt ctttcagctt
     7681 tcttttcgtc tcttgatttt
//
